LOCUS       DAOTFD010000001        48103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_535_length_48103_cov_4.838120, whole genome shotgun sequence.
ACCESSION   DAOTFD010000001 DAOTFD010000000
VERSION     DAOTFD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 48103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48103
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_48103_cov_4.838120"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1510
                     /locus_tag="P5079_00005"
     CDS             116..1510
                     /locus_tag="P5079_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRY28398.1"
                     /translation="MNSFAPAQLPVENIHFDKKNPRGESEEVILKDPEFEKLVSSIKE
                     FGILEPLVVKEETKQKDTYVLIDGERRLRAAKKISLPKAPAIIADSDTNGRVLAYQIH
                     MLRKQWEKASETKSIKSIIADLMQENPKISDGDLKKKLLEITKHKDHEIDDLMNLCKY
                     DDVIIDKVLRDELDMSYLIQNEKSFITPLKRKYPDLVATYTEDKIRKIMAQKALDSKL
                     VKSRYLMDTFKYVFRDEKNHQKIKRIIKRFLNSKDKSIETAFNEYLETDKALMKKFGL
                     GHKSKSKSTKNKPAAAHQLILKTTPQQQTKLADIEKRFLRSSLTSEELAYIKEAFSCM
                     RAACLKASVVMIWATGISRIIDFISNNISKYNSAVDRMKELADKKCKPYNYTHYRKVS
                     DEDGLRDGNDYQLLNYLLYEKIIHMADYKQLKSAYDTRCDCAHPTNIDLKVNRVLTIF
                     EEICNHIFLNKSIK"
     gene            1507..2607
                     /locus_tag="P5079_00010"
     CDS             1507..2607
                     /locus_tag="P5079_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HRY28399.1"
                     /translation="MKTLLVEPDFPIPSKSKNHSNFLPVGLLKIAAYHRHLRDRVELH
                     RGNYPALRYPDEIKITSLFTYWSAYVWESVRYYKNLYPKAKIELGGIYASLMPEHAKQ
                     TGCDTVEPGLYRNGVAENYIPAYDMVDVDYQIIHASRGCFRRCNFCGTWKVEPLVSFK
                     KTIQPEIKKKKLIFYDNNLLANPHIKNILGELAEFRFPNRCTVECEGQSGLDGRLLTS
                     DPGIAVLLKKAHFKNPRIAWDGPYLMWPQIKEQIFSLRTAGFSSDNIFVFMLYNHRLS
                     YDEMRAKLDACRRWGVRVIDCRFRPLNSIQDGYRPRAKGQKATDYFVHENWTDRQVRT
                     FRRAVRRQNIAIMLGLPNGRYLDGIEEKYIQV"
     gene            2947..3498
                     /locus_tag="P5079_00015"
     CDS             2947..3498
                     /locus_tag="P5079_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28400.1"
                     /translation="MLEALRHREQEIFQYLAILGPALGGFIWLCYKKEAASAFVPGTI
                     GVLLALLLGAIYSLALGYNFRYIVFELAKLEIILGMKDAMLLAWPRSVEAFRKSAFLH
                     HRWMPIPWVTPPEIIKIFWISFLVFILGVTAAAQRFKPIGISEEHTKLIIYVGGVSFL
                     AGILTPILFGWKFLDKCKKERGW"
     gene            3626..4369
                     /locus_tag="P5079_00020"
     CDS             3626..4369
                     /locus_tag="P5079_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRY28401.1"
                     /translation="MGSSTGSLAASIAEFQQYLKAKVAVHIETEHKNLYPFITISREA
                     GAGGHLLAQAILKRMEKESLPVYQGWHMLDQEICEAVLADPKMKTSLEPLLSERFNSE
                     LNDYLSQIIGGTSPQVAVYTKIFRLIMSLASVGKVVVVGRAGSFVTRLLPLGIHVRLV
                     AEKEQRIQYMMRERDLPRDKAETTVAQLDQDRQRLVKTFFNRNAADPLEYDAVINTSR
                     VPLESAVDALIALVKQRFEKAPAVLASKA"
     gene            4542..4985
                     /locus_tag="P5079_00025"
     CDS             4542..4985
                     /locus_tag="P5079_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRY28402.1"
                     /translation="MKFSQAKQGRTFIIRLEDGDIIHREIEKFAQTHSVRAGFLVVLG
                     GADTDSTLVVGPKAGRSKPIVPMKHLLDNVHEVTGTGTLFPNEKGEPVLHMHVACGRK
                     KATVTGCVREGVRVWHVLEVILVELLDTSAVRAFEPDSGFELLRP"
     gene            5361..5433
                     /locus_tag="P5079_00030"
     tRNA            5361..5433
                     /locus_tag="P5079_00030"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5394..5396,aa:Ala,seq:cgc)
     gene            5670..6347
                     /locus_tag="P5079_00035"
     CDS             5670..6347
                     /locus_tag="P5079_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28403.1"
                     /translation="MVKRNTLKAFVVFGLCCGYGPAIGRADGAGELRPEWQIALELSE
                     ITATDSATFLHHQSMPWLSVSHTLSPRWNVGAELAYLDPKKEPITGLFTQTGSWSLNY
                     KLSGGYLGPYMEALLQGSRFGASFRCGVGVTYLRQSWEGQGISTQNMSHYDPSLVFSV
                     GGSCRIGSNSHLHVGLKNLIVFQHGEYLTTTGFRGGSPNGPILEKEAVDTPEHLFLPF
                     IGASVRF"
     gene            complement(6523..7014)
                     /locus_tag="P5079_00040"
     CDS             complement(6523..7014)
                     /locus_tag="P5079_00040"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY28404.1"
                     /translation="MMGKIKIYDKLFGSGPVGVLLSLVLLSAAHQLNRRIHLPPVTNN
                     RFLPSLAFFVSILITAVIIVWSVKSLPVADRGNKLCTTGAFKYVRHPLYAAFLSVFNF
                     GLAVYLNSYLYLLWAVLLHPLWHLLIRSEERMLVDLFGEGYIEYQKKTGRFFPRLFRR
                     GTG"
     gene            complement(7080..7259)
                     /locus_tag="P5079_00045"
     CDS             complement(7080..7259)
                     /locus_tag="P5079_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28405.1"
                     /translation="MAKKTRPFAGPARAVKRSFPDTAHNFWDNHRPAEEAGAESTKLW
                     DFHFSSNMRNMENTG"
     gene            7385..8431
                     /locus_tag="P5079_00050"
     CDS             7385..8431
                     /locus_tag="P5079_00050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY28406.1"
                     /translation="MTLPLEQRLRAYVDPWLKDFNVLGVAVHITGDRPLPLFAGFADP
                     EKKRPLSVESAFPAYSASKSYIAAVVLRLAQDNLLGLDDPLRRWLPDLPFDKPVTLRQ
                     LLNHTSGLPDYRALNSHKAGMAQSPSAPWDEPRFLEAVFAEKFLFEPGTAWLYSGTGY
                     LLLRKAAEAAARRPFSQLLSEQVLEPLRLRRTFLLNGPADMRRLTPGYARQFNPAGRP
                     EDVRAVFHPGWIAHGVVGAPLEELNVFFDGLLNGRLLSPDFVSEMKKPVLIPFKHPFF
                     ASPGYGLGLRYYDDPRFGKIFGHGGEGPGFAGFSYYVEARKTLVSLYCNTDVRARFLE
                     KLMFQLLSVLHDLL"
     gene            8447..9358
                     /locus_tag="P5079_00055"
     CDS             8447..9358
                     /locus_tag="P5079_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY28407.1"
                     /translation="MTPRSSRSSSVKAYLALGAGILCIGFSAIFVKFAAVPGPVSAFY
                     RTFVASAALLPWWLGRGPRLPGRKDMRLILLGGVFFAVDLVLWNSSLLLTPAATATLL
                     ANNAPLWVGLGSLWLFREKLPGLYWVGLALSLAGMTILIGETAWYSHRLNAGDLMALA
                     ASGCYAVYLLSTQHARAHVDTLTFMTLSTLTSLALLGAGVALFGLSFSGYSAKAWMSL
                     LGLGLISHLAGWLAINYALGHLRAAPLSVSLLGQALVTALLAIPLFGETLRTHQLIGG
                     TLVLAGIFLVNHRRTRAEAGPTAPENV"
     gene            complement(9368..11041)
                     /gene="ettA"
                     /locus_tag="P5079_00060"
     CDS             complement(9368..11041)
                     /gene="ettA"
                     /locus_tag="P5079_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRY28408.1"
                     /translation="MAGEYVFVLSGLSKSYGPKVVFKDIHLSFYHGAKIGIVGENGTG
                     KSTLLRVMAGVEKDFIGSAEPKKGVRIGYLPQEPRLDPAKTVRQEVESAFKHITDLVA
                     EYEKLSDSMSQPMSDDAMEKTLEKMGRLQETIEAADGWDIKRHVDVAMDALVLPPDDL
                     ATTKISGGEARRVALCKLLLQKPDILLLDEPTNHLDAETVNWLEGTLRDYPGNVIIVT
                     HDRYFLDNITRWILELYRGEGFPFEGNYSAWLEQKSKRLELEEKKASSLQRRLKEELA
                     WMHLTPSARSGKSKARIREYETLVSHQKTVDVNSLDIQVVPGAPLGAKVIEARHLFKG
                     YGDNQLLQDVSFDIPRGAVVGLVGPNGVGKTTLFRMIVGEEKPDKGELVVGPSVSLSY
                     VDQKRETLDETKTVFQEISGGVDHLDIGGKLVPARVYLSRFNFKGTDQQKKVAALSGG
                     ERNRLHLAKQMLKGGNVLLLDEPTNDLDVDTIRGLENAIMEFSGCVLVISHDRFFLDR
                     ICTHLLVFEGEGELRWFEGNFQEYQEVRRRELGGREESRRSKYKKLTIK"
     gene            complement(11132..11710)
                     /locus_tag="P5079_00065"
     CDS             complement(11132..11710)
                     /locus_tag="P5079_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HRY28409.1"
                     /translation="MIGKMSWKQGLVCVVLPFFVSCAKPPTQEMSDAEAAVTAAVQAG
                     AEQYAKTELAAANDALADARAKLESKDYKGAKAAALETKAKADVAQSAVEPNKAAVMT
                     TVQERMNGLKPEIDAVQSAASKLKGRGVEPIKAEAAALTDLWAGVQTAFDGGDYNGAL
                     QKLDELQPQLTAARTSVDAAAKARLPGQAKKK"
     gene            complement(11887..12294)
                     /locus_tag="P5079_00070"
     CDS             complement(11887..12294)
                     /locus_tag="P5079_00070"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRY28410.1"
                     /translation="MKRALFVVGVVSLSAGLLFAGDMKMPSVGKVSAEPPAAAPAAEK
                     FTCSMCGGEYTGAGKCAKCGMDLVKKTEEKQVYSCPQCGKESDAPGKCMKCGVEMKVK
                     EIKAKAEEISTQVQEAKPALEKAKEKVKSKKKK"
     gene            complement(12334..12690)
                     /locus_tag="P5079_00075"
     CDS             complement(12334..12690)
                     /locus_tag="P5079_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28411.1"
                     /translation="MKHLLCAFLLLFTGFAGAFLGKSRSGAAPAWARWRGLFQLLFPA
                     GFLGLIVYVSFSYRWYVSLLLIALQFGVVSLFRKIFLSGEDRSQEDLSFRQGLQENLK
                     RLQELNAAKLRERSKK"
     gene            complement(12704..13696)
                     /locus_tag="P5079_00080"
     CDS             complement(12704..13696)
                     /locus_tag="P5079_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRY28412.1"
                     /translation="MNFSEGNEVAVGCAAWLDRSLLKDGDFYPRKTMTSRDRLAWYAR
                     FFPFVEVNSSYYALPAPPTVSQWAEATPPGFLFGVKAWGPLTGHTVQALAVPPALRRY
                     FLNVPRNARGEIEARHFPPSAREASFELLREAVSPLVRSGKLGYLLFQMAPWVSDTAE
                     GRRALAALPRRFPSIPVAVEFRHPDWIPGRTEDTLKFLEDNGLSHVAVDAVWMPRVAA
                     ATGPVFVLRCHGRNEAGWRAQKEGRAPTVAEKYDYHYSDGEVDELVAAVRREAGPRRR
                     AFLVFNNNNKDYPVRNALSARKKLGQPVPDLAAAQKAWRSDRSPRDPAAELFKW"
     gene            13814..14308
                     /locus_tag="P5079_00085"
     CDS             13814..14308
                     /locus_tag="P5079_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY28413.1"
                     /translation="MDALEAIARRYSERSYTSKPVPKDVLEKIVDAGRRAPTARNVQP
                     WEFVVVTDAAARKQIAGLAENGKFIEQAPACVAVFCKDTKYYLEDGCNATTALMIAAA
                     ALGVQSCWVAGDKKPYGGAVAERLGLPDGYKLVSLVPLGYAAAAGRPPAKRPLDEVIH
                     WEKF"
     gene            complement(14315..15229)
                     /locus_tag="P5079_00090"
     CDS             complement(14315..15229)
                     /locus_tag="P5079_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY28414.1"
                     /translation="MRRLRRLPVWIGKGVLIVLLAEIVLQLTGRLYYATHRHFLQPPS
                     ETAATADFVILFVGESTTAGDGAEDLRTGPFPAQLEVLLERRFPGMDFACVNLGLKGT
                     TTPYLLNKLKTDLDAYRPDLVVIQAGNNLPAPHFMLVPVVRLGKWTWEDVVFFLQRSR
                     IYRFCRLVGEILWRGGSERLYKAVNDNEDIPRRMRSLLVHHSVADIRNMVRTARRQGV
                     KPVLCNYFESRSNPFLARLAAELDVPLCDNQLVFGRLLKRGEARNYLSADGWHPNTAG
                     YRLIAENLLRTLIEKGLLPRTPPRSGAA"
     gene            complement(15233..16348)
                     /gene="proB"
                     /locus_tag="P5079_00095"
     CDS             complement(15233..16348)
                     /gene="proB"
                     /locus_tag="P5079_00095"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRY28415.1"
                     /translation="MSSPTSRNRPVVVKVGTAMLSRPQGGLNTARVRSLAEELVALQK
                     RGSPVVLVTSGAIGAGMDKFGWKTRPTVLSDKQAAAAVGQVVLMEAYEKAFGRLGVTV
                     AQVLLSRSDMEDRERYLNARNTLSALLSRGVLPIINENDTVATEEIQFGDNDSLAALV
                     AAKVGAGKLFLLTDVDGLLSGREQDARLLPEVFQITSEIEALAGAKGGSHKSVGGMSS
                     KLRAAKLAMASGVEVWIASGKRPGVMGEILQGHGVGTRFQARADSLNARQRWIALGRR
                     VKGTLVLDAGAEAAILQNKRSLLPSGIVRVEGTFHAGDTVKLLNGSGVEIARGLTAYS
                     SEEAKKIRGRKSIELERLLGRPCPAEVIHRDNLVLLT"
     gene            complement(16308..17576)
                     /gene="obgE"
                     /locus_tag="P5079_00100"
     CDS             complement(16308..17576)
                     /gene="obgE"
                     /locus_tag="P5079_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRY28416.1"
                     /translation="MFTDKAHIIVSGGHGGDGCLSFRREKYVEMGGPDGGNGGRGGDV
                     WLEVDPHKTTLADFAYRPHFKAGSALSGHSANKTGRSGEDLVIRLPPGTMVYREGKLV
                     CDLKNPGERFLAAHGGRGGRGNASFKTSTNTAPRIAEKGETGESAELDLELKLLADVG
                     LVGFPNAGKSTLLSRITKAHPKIADYPFTTLSPNLGVAHVGERSFVVADIPGLIEGAH
                     QGKGLGDEFLRHVERTRVLIHLVDVSGWDGKSAYQNFCAVNKELGAYSERLLQKPQVV
                     AATKMDLTDAEKKRTVFARRLKKTRVHAISAVTGLGLAPLLAAVLKALDKAPDADLFV
                     PAPSALYRLEPDFWVEKEGELFRVRGKGVERLVAMTNFDQDEAIARLQKIFRKMGVEK
                     TLEQKGIAAGDLVAIGDYEFTYKPEPPRRR"
     gene            complement(17674..17964)
                     /gene="rpmA"
                     /locus_tag="P5079_00105"
     CDS             complement(17674..17964)
                     /gene="rpmA"
                     /locus_tag="P5079_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY28417.1"
                     /translation="MAHTKSQGSSTNGRDSQGQRLGVKCFGGELVTAGAVIVRQRGTH
                     FAPGRNVGIGSDDTLFAKSAGVVRFEYLRGTLGRRGGRRRVSVYPGTAPAAS"
     gene            complement(17966..18295)
                     /gene="rplU"
                     /locus_tag="P5079_00110"
     CDS             complement(17966..18295)
                     /gene="rplU"
                     /locus_tag="P5079_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY28418.1"
                     /translation="MKSDSYAVIRTGGKQYAVSPGDKIRVEKLPQNQGEEVRFGDVLF
                     VKSGDQARAGRPTVAGAEVVGQVLRQMRDKKVLVFKKRSKKGYKKLQGHRQFLTEVLI
                     KTVQAGN"
     gene            complement(18348..18779)
                     /locus_tag="P5079_00115"
     CDS             complement(18348..18779)
                     /locus_tag="P5079_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY28419.1"
                     /translation="MGHISWNDGQAIFQKAHVLASEVYRSTRTFPISEGSGLVYEMRQ
                     SSLAISAFLSDALRKDQKSYLVDVLETVLKHLADLRRNVVVSRFLTYLSDDDFEKLDR
                     LAEVFRADVETWKESLDTFSRPGDRRPGDPAPTRLSVHRSA"
     gene            19095..19898
                     /locus_tag="P5079_00120"
     CDS             19095..19898
                     /locus_tag="P5079_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28420.1"
                     /translation="MVDTAISIFSVFLQHATRLNEALPGDPTVRTGVETVLCLFLMTL
                     LYRVWRERILAEEGTFDGELWDRILSLVFQHRTKDAAALAETTPGVFAELYLAALDYS
                     AQGREDAADAVESVLQEHRQRQGRARALLAGGAAACLAAGAAGSAISLMPAAEGWSAA
                     GLNALTNFSPDLWPLMAGISAAAVTGLFRSGFTRRGERLAAHLRQCGLKLLATLLESS
                     RDAVKKEAAKPVPAPKVAPEAPLPLPSAPKVLPAPTGLSVWETLTQHHL"
     gene            complement(20001..20684)
                     /locus_tag="P5079_00125"
     CDS             complement(20001..20684)
                     /locus_tag="P5079_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY28421.1"
                     /translation="MASKILVVEDDPDVMAFVRAGLSFKEYDTLEAMNGRDAVAAAKG
                     KLPDLILLDLMLPDMDGMEVCRQLKADEKTKSIPIIMITARHDVADIVRGLETGANDY
                     VTKPFDVMELVARVRTQLRLADERRGFPKMIERDGLSLDWFQRTVQHEGAVVSDLTDK
                     EFAILFHLVLYSPKLLGRQEIFENVWGKEYKGTSRTIDVHIQRVREKIGSKLSRRIIS
                     IKGQGYKFV"
     gene            complement(20702..21772)
                     /locus_tag="P5079_00130"
     CDS             complement(20702..21772)
                     /locus_tag="P5079_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28422.1"
                     /translation="MKSTCSLLIPFDLGLELDFEGKDAREIVKEVSSRPAAPLTFEGR
                     IFSNATITTRIYKFGVGLIQLSFPVEGDIGFLTALSCRAEAIAVGKTAVSTWARTLVD
                     GLIERARKFASHTYERRLEEVDIFPVFVFEKGVVSSAGEFVKRNHKALFGIVSGEANY
                     DDLSDFALEQERLVNFGYYENELILIKRFGAVVSSGEAHTILELIRLAYALFWNLRGY
                     NFFLDREIDSAQALISRLPPYYKVWVMPQRYQMFSREAMGFGIDKLAIVDSLYNVSAN
                     IPHIDSDWHLRTIYRNVQKEFDIDELSKMVEVKLERIEDTYNSARDFLSTNFFIAVEI
                     VLILSLFWMVLDTSLLFTIAQK"
     gene            complement(21783..22112)
                     /locus_tag="P5079_00135"
     CDS             complement(21783..22112)
                     /locus_tag="P5079_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY28423.1"
                     /translation="MMNDLRAGPRLSTSLPVILQFPDGVPTEGWGRILDLSLSGVKLE
                     SRWPLKVGQAVYITFEPQHEMRLENLRARVVRVQWEEGYYVAGLSFDESVDQAYLREA
                     LISLMSR"
     gene            complement(22109..23245)
                     /locus_tag="P5079_00140"
     CDS             complement(22109..23245)
                     /locus_tag="P5079_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28424.1"
                     /translation="MSQVLIVARDPAVRDQLKDVARQAGVTVDLLEDFDPAFAAIERE
                     PPVLVFAENPPSDDVMEQLRKTLKVHAPVTPLLLYLPERNSALALKRMTEGAYDCLSS
                     PVTPGDFLAAAKRSVSRLGRRLLTSRQVRPAVWWRRPAVVAAFSVLMVAGFLVLGVLR
                     FWSPPFQIFKLASDHPVAVGGDEDTLWVADWSQQNLTRVAVRGDYLSIVQVNRFEDFQ
                     PVASALAPYYAYTASADGRLRRHRLDDALSSVALVPSPGPSPSGLAWDGAALWSCDSY
                     TGKIYEHDARLGVKNIFDGPAAKPVGLAWVDDTLWVADGEHNTLWRLNRTGVDWEKKG
                     PFAPLVFAHNRNFQLSGFTVWKGSVWIVSEAGGVLIKERLPKVK"
     gene            complement(23242..24657)
                     /locus_tag="P5079_00145"
     CDS             complement(23242..24657)
                     /locus_tag="P5079_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY28425.1"
                     /translation="MADEKKEPLPTILPADLKPKLDWPALVHQTKEKLAKILWATEGI
                     SDGARELALKEVLWELEQFQKKVEDVAGRWESVERIFREENELLRSLLGTGEHELRAR
                     VLALAQEIHNLRKDLLTSRDEAAALTRKAGSYAESNEDLRRQLGAAQQRIAELEAEKS
                     HEWHSQMSDFSREQSALQEQFTKLNEDLLQVQSMLSVKSREMTSEKQAELTALQTKLL
                     QEMEEALHQKEELLWAEEEIFARGVAQKLRGEMQSAMGRLQLTLEKFHLLEVQDKPGA
                     GTWEQWWRLIKVGPDELRRGFQEVAQELRRGVETLEEYLALTHRRVPARAELNLPEIV
                     HAQVAKLYTDRLEKGTLEVIVPEVFPVFMGDGELLAAVLQALMDNAFESLPRAGGRVR
                     LRAEKSPDEKELWITVSDTGCGIPQGQRDRLFQPFFTTKDGHRGLGLARAKRYAEWHH
                     GRLELVDSGAEGSAFRLVLPV"
     gene            complement(24662..26044)
                     /locus_tag="P5079_00150"
     CDS             complement(24662..26044)
                     /locus_tag="P5079_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY28426.1"
                     /translation="MLGGCVALFLGAVSPLWGRNRPLPTAGARAMALGGAYSAEDQDV
                     NALFYNPAGLTGLTGSQMSLNYGRLNVGTLNTTSEIHGAFGMPASFQGRPIGAAGGFL
                     AQSLAPGAHVFDLYSGFGMDVPTRGRVPWPVRGGGVLKIRQQNGDEKDDAVGKTKVGF
                     GLDGGALVQFNEDFTLGFVLRDLFPSGVNPPGPQLRLGGKYRYVQKVILLSDLEVRRN
                     VTALHFGAEWLFYRDLLRLRIGNSFRANSANHIALGAGFNFSPAQIDVAYAVPTEDFN
                     EPSDQFRITLIYRFNAPRFSELYFDRALDMADEVDRRVAQLEEKESQLKTSVQDLEQA
                     RRLAEEELARVSVRRAEGQRDVEERILQAETRASEAERRAADLEDKVRVSEEKIRRAE
                     RLTAPRPASTEKPKPKIRTHVVQPGDSLRSLAAKYYNDPERWKTIFNANPKKVDRGRL
                     LPGAELVIPE"
     gene            26286..26837
                     /gene="amrA"
                     /locus_tag="P5079_00155"
     CDS             26286..26837
                     /gene="amrA"
                     /locus_tag="P5079_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRY28427.1"
                     /translation="MFSIEDQHAILQQARSAIAGRLGISVPAATLRPELADAAYALFV
                     TLHAKGELRGCIGLMRPEGKFSRSLTEMALAAAFEDPRFSPLTQREYPDIEVEVSVLS
                     PLQRVSRAEEIEPGRHGVMVRRGPLSGVFLPQVWETLRERETFLGTLCRQKAGLPENA
                     WREPSTELYVFTVEILREDGGAE"
     gene            26834..27268
                     /locus_tag="P5079_00160"
     CDS             26834..27268
                     /locus_tag="P5079_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28428.1"
                     /translation="MTRRRFQILFFLVLLPSSARPTETTRASLPSAPGKIKAWTEGLF
                     RLQYVLAAEGASTAARTVRVTLKNDTRYVTALSPRLTEHEAVHRRINEIAARRMETEL
                     EGFRTAGDSLKDGERLFLAAFRRKIAEAQELHRAWDATHTVP"
     gene            complement(27390..28481)
                     /locus_tag="P5079_00165"
     CDS             complement(27390..28481)
                     /locus_tag="P5079_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28429.1"
                     /translation="MLKPSLRLVFWESTAACNLRCLHCRRLEVRDTFTQADMDTAAAR
                     RMIVDIVAVGRPILVFSGGEPLLRGDIYDLAAFAREQGLPTAVASNGTLLDEEAAVRL
                     RGAGVGRVSFSLDGASAPAHDRLRGPGNFEKTIRNVSFLKAEGLGFQINTTVTRRNAQ
                     ELPALHDLAVRLGAKALHLFMLVPVGCGAQIADGEMLSSEETETWLSWLHDRASERRL
                     ELKATCAPQYTRISRQKSKRPGSPPGSPEPPVSRGCLAGVGVCFVSHRGDVFPCGYLP
                     LRAGNVRTQPFREIWESSPVFQKLRDPEFLEGKCGACSFKVLCGGCRARAYYQHGSEL
                     GEEPTCAHVPPARAPRTNGPVPRRRSSSK"
     gene            28559..29230
                     /locus_tag="P5079_00170"
     CDS             28559..29230
                     /locus_tag="P5079_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY28430.1"
                     /translation="MAVWNPEKFFASLPFFRNVPVSELQKFVWQSRRMPFKKGDYLFQ
                     EGEPAEAAWWVTEGFVKIAKSTPQGRLITMEMLMPGEIFGPAGVIRMSHYPASAVAVT
                     KGAAARLPGEALAALARACPSVAQGVLTEVSQRIQRAHRLRALDAESADKKVAAALLW
                     LREKTGSPLAVPRREIAEIAGVAPETAIRVVLQFKEKGWLTAKPGAIALVRPEHLRKL
                     VDEGP"
     gene            29227..30486
                     /locus_tag="P5079_00175"
     CDS             29227..30486
                     /locus_tag="P5079_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28431.1"
                     /translation="MTPAAPESLTLGLTGVSLSLVVSPQATLTFDFSGRPIGLYQNGS
                     YYAFGLDGRAIEKKWRPEDAERRKRYLRLLSTDESRRLRERLVDILHACEERLPELQA
                     GKRLQLWFNGKRGVATARRAGRVLRHLLAFNTVRWRTDAEQFRKLYPALGILPPDQYL
                     ALVVQVTEGCFWKRCAFCVFRPETPARVKSRDEILRHIDAVRGYWGRALPRRCSIFLG
                     DANAFDAPPDLLLWTCRTLAEIFPRLAVPQKDGFGGLYAFGEIASLSTWEDADLAEIS
                     RSGLRRVYLGLESGSDTLRRWSGKPGTARQALETVRRLKNAGLDVGLIFLLGLGGREN
                     GREHVETSARFLREAPLGRRDIVYFSPLREDFSSEHGPRLKENRWTNLSEEGQWDQML
                     ELRRPLDERPPTERPMCGLYDIREFLY"
     gene            complement(30535..31689)
                     /locus_tag="P5079_00180"
     CDS             complement(30535..31689)
                     /locus_tag="P5079_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY28432.1"
                     /translation="MRNSGGIYFDNAATSFPKPPAVLAAMVSFQKKIGASAGRGVYPR
                     AVETGRILASGRRALARLLNVADERRLVFTLNATDGLSLAVKGLPWKRGDRALISPYE
                     HNSVLRPLHELKKRVGVGIDVMPVDGEGRVIVEKLPAALRPRTRLVACVHASNVTGVI
                     QPVEAVGRFARSRGIPLLIDAAQSAGALPVDVRAFRADFLAFPGHKGLLGPLGTGALY
                     IRPGRELQTLREGGTGSVSEVETQPLFLPDRYESGSHNALGIAGLLASATYVRRRGVS
                     EIRRHELELTEHFLEELRRMRAVRYFGPRAAEDRVAVVSLRVGDRDPLAAARALWRND
                     GIMVRAGLHCAPWAHRHIGTFPHGTVRFSLGPFNTHRHIEKAVAALQKLI"
     gene            31834..33192
                     /gene="lpdA"
                     /locus_tag="P5079_00185"
     CDS             31834..33192
                     /gene="lpdA"
                     /locus_tag="P5079_00185"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRY28433.1"
                     /translation="MKKLSVVVLGAGPGGEAAAKKAAAHGAEVTVVEKADLGGLCLNR
                     GCVPTKTLLEAGRLLNRARAASAYLKGSDGLRVEWDALQKKKTEVVSLFRKSIADAFA
                     RQKIKLLRGQARFLDDHTLVVHSDGVEDRLSFDRAIVATGSVPLVPPPFPAVEHDLLD
                     SDKALGLARVPKRLLIVGGGAIGCEFACLFHELGSRVVLLEKTPALLPGEDAALVQVL
                     QTSFEQRGIQIKTASTVDHLERKDGVWRCRLGGGETMEVDEVLLCVGRRPMLTGLGFE
                     NAGLRPENGRLPVNEFLQTSRPHIYAVGDVNGHSLLAHAAAAQGETAADHAAGVQTDP
                     YDPLQVPRCLYTWPEAASVGEWRHTAETKGFVVKAHRFFFQGSPKAWASDEATGFLQL
                     ISDKNTGRILGAQIVGPHATELIHLVSVALRAKLTTDQLRSVVFAHPTLSESLRGALS
                     RG"
     gene            33235..34329
                     /locus_tag="P5079_00190"
     CDS             33235..34329
                     /locus_tag="P5079_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY28434.1"
                     /translation="MPLLKKYVSAAALLLSGVLAAAALAESSLRLVDTRLHRDILREY
                     DARIIYRRTQSYFSDRPSSPEGSRFVFVSPRLPLRYSAGAIETAGGPLRYRKNGLLDD
                     APVHGVVLGDSYSENLYLDDSEGWVSLLSQATRKRFLNLARTGTGTEHHLRCAELILP
                     VARADVVLLQICFNDVLDDNRRADRTHPSHPRAGLFPRPDRLRRVVAETFPFNRSVFC
                     FMFARWGMEFIGTRFFSPPLTPPIPETYPPDELARTTNNIAAIKGLSERHGAHLIVIA
                     FADDHFLETLGLPAWSQTHHVPLVVLNRSSSLWQDVPPSADFSAHLFLTNPHFNELGN
                     RKIAAHLQRVLQEKPSADAVQLPRDSGIPG"
     gene            34326..35237
                     /gene="lipA"
                     /locus_tag="P5079_00195"
     CDS             34326..35237
                     /gene="lipA"
                     /locus_tag="P5079_00195"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRY28435.1"
                     /translation="MNGPRQIRSIAKAPPEDRPKKSWRPKLLPHHKQSIPAGRKFFHV
                     TRLLEESGLSTVCQGALCPNRAGCWSGGSLAFQILGDLCTRRCAFCGEKTGRPRPPDP
                     SEPEKLAAMARRLGLAHVVITSPSRDDLPDHGAGQFVACLEFLRKEIPRTTVELLVPD
                     FQGRPDLLKSIIQMRPDIFNHNLETVRRLTPEVRARALYDRSLQILRWAAEAGLRVKS
                     GLMVGLGETREEIRETLTDLKRNGVSLLTVGQYLPPSPRHRPLDRLYSPEEFSSIREE
                     AAGLFENVLAGPLVRSSYHAAEMAGAR"
     gene            35234..36301
                     /gene="gcvT"
                     /locus_tag="P5079_00200"
     CDS             35234..36301
                     /gene="gcvT"
                     /locus_tag="P5079_00200"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRY28436.1"
                     /translation="MNRTPLYPLQKQLGARFMDFGGWELPLQFKGILAEHKAVREKSG
                     LFDISHMGQVWAEGPGAESFLNGLLTNDIAALPPQKGLYSVMCRENGGVIDDLYVYRL
                     TTDRFLLIVNASRKTADLDWIRRHRPGGVNISEQPEAAAVALQGPDSPDLLNLLCAPA
                     LALGRNGVGAFTIEGSGCVIGRTGYTGEDGFEIFASAENLRRIFQKIWELGEPRGLNL
                     CGLGARDTLRLEMGYRLYGQDLDEDHSPLEAGLGWTVRLEKKEFIGRDALREEKERGP
                     KRRFIAFRPTEPGVPRHGGKLSAGNQIAGEVTSGTFSPSLQAGIGMGYVESRFAAAPL
                     AVEIHHRDVPVSIVKLPFYHK"
     gene            36333..36710
                     /gene="gcvH"
                     /locus_tag="P5079_00205"
     CDS             36333..36710
                     /gene="gcvH"
                     /locus_tag="P5079_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006991820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRY28437.1"
                     /translation="MKPEELRFVETHEWIAPDGTVGISDHAQHEITDVVFVELPKPGR
                     VVKSGEAVAAVESVKAAFDIYAPVSGTIAAVNESVTKTPGLVNQSPYEAGWLFKVKAD
                     REEERNGLMTHETYQTFLKSETH"
     gene            36750..38075
                     /gene="gcvPA"
                     /locus_tag="P5079_00210"
     CDS             36750..38075
                     /gene="gcvPA"
                     /locus_tag="P5079_00210"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRY28438.1"
                     /translation="MFVPHTEEQKKQMLDQIGVRRFDDLLADLPSDIRRPPLNLPPSL
                     SEMEMVRHMESLAAKDHLALSFLGAGAYEHFIPSAVWSLALRGEFATAYTPYQAEASQ
                     GTLQSLYEFQSLICELFGMDVANASLYDGASAAAEACLLALRHTGKRRIVIPETVHPH
                     TRQVIQTYLAHSPADIVTVPRREGTTDLGRLQAALGQETAAVLFQTPNFFGCLEESHE
                     IARLAHAAGALLIASSYPTALGLISPPGEYGADIAVAEGQPLGLPLGYGGPYLGLFAC
                     KTELMRKMPGRVVGRSVDRDGRRAFVLTLQAREQHIRREKATSNICSNQSLCALAAAI
                     YMSLVGKSGLKELALLNLQKARYASKRLAEKGFPTAFPQPFFNEFAVKCPKPPELIAE
                     RLAKDKVLAGLALGRYFPELKDHLLVCVTEIKTRQDIDCFADLLERACR"
     gene            38066..39538
                     /gene="gcvPB"
                     /locus_tag="P5079_00215"
     CDS             38066..39538
                     /gene="gcvPB"
                     /locus_tag="P5079_00215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRY28439.1"
                     /translation="MPIETPPPLEPLIFELSVAGRRGLPWPKPDIAEAENESPLPAAL
                     RRRTPPEWPEVAEPQVVRHFTRLSQLNFSVDTHFYPLGSCTMKHNPKAADRVVLLPDF
                     IGLHPLRPHTLSQGILEILHDTERWLCAICGMDAFTLQPSAGAQGELTGVLIARTYHE
                     SRGQNRPVVLIPDSAHGTNPASVAVAGLTAVTIASTPDGHVDVDVLKSKLGPDVALVM
                     LTIPNTLGLFEPRLREIAKMVHDAGALLYMDGANLNALVGLLRPGDLGVDLLHINLHK
                     TFSTPHGGGGPGAGPVGVKAFLSEFLPTPRVVKEDGVYRRRMDFPKSIGKIHGFMGNV
                     GIVLRAYCYLRLLGEEGIRHVSENAILAANYLRVKLAPLFPKMPKKPCMHEVVVSGEG
                     AFGPGVHTLDVAKRLLDEGFHAPTIYFPLIVPEALMIEPTETETKETLDLFCKTLESI
                     VEESRTSPEKVTSAPHRTPIRRVDEVKAAREPDLRFKRIP"
     gene            39548..40159
                     /locus_tag="P5079_00220"
     CDS             39548..40159
                     /locus_tag="P5079_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28440.1"
                     /translation="MSHDWRLLHDGALSGEENMRRDRELLENQRRPDARPTLRFFRWS
                     SPTLTYGKLQGEGPARQAAQAFGAAHVVRRPTGGGMVLHDKNLSLSLTWPRRSRAFPA
                     SSRSLYRTVHQILKTALRECGTETSFFVPETSRKAGPGLCFSEPVTDDLMWRGKKVAG
                     GALRATRWGFLYEGDVRTELVDIAPEMLIEKITAAFRNYFSKG"
     gene            complement(40168..40857)
                     /locus_tag="P5079_00225"
     CDS             complement(40168..40857)
                     /locus_tag="P5079_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY28441.1"
                     /translation="MSRIKAVFFDAGGTLFRPHPSVGHVYAGLARKYGCDASAEWVDR
                     RFRAQWRRRNGLEFLKNTDEGEEKAWWSRLVRAVFGRRMSEETFRPYFEELYDLFAQP
                     KTWRLFPDALPTLKKLKKRGVPVGIVSNWDSRLFELCRGLGVAPWVDFILASAVVGSV
                     KPHPGIFQKALKLAGVRPSEAIHVGDSFREDYCGATRAGLKAVWLCRAGGGSRRAACI
                     GSLADVGEMMS"
     gene            complement(40854..41258)
                     /locus_tag="P5079_00230"
     CDS             complement(40854..41258)
                     /locus_tag="P5079_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRY28442.1"
                     /translation="MARGTFNDLIKIMARLRGPGGCPWDRRQTHRSLLRYLREEAGEV
                     ARAVRRKDDANLCEELGDVLLQVVFHAQMASERRKFDIRDVVAGLCRKLVRRHPHVFG
                     RKRLKTAAEVVVLWRELKRREKARRTRRGRRG"
     gene            41389..43350
                     /locus_tag="P5079_00235"
     CDS             41389..43350
                     /locus_tag="P5079_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRY28443.1"
                     /translation="MLEKIKELLFGKPKSPLDPKVFHKVSLIAFFAWVGLGADGLSSS
                     CYGPEEAFLSLGHFTHLALPLALMTAVTVFILSASYSQIIELFPAGGGGYLVASRLIG
                     PKTGLVSGCALLVDYVLTVAVSIASSMDAIFSFLPHDLLRWKFLATLLCLLLLIILNL
                     RGVKESVLILTPIFIVFLITHLLMIFYGIFSHGAELPALMGDTVQETRLGVAELGSFA
                     MLFILLRAFSLGGGTYTGIEAVSNGLAILREPRVKTGKKTMMYMAISLAFTAGGLLVA
                     YILNNVHHIPGRTLNATLFYTLTAPWPLGNIIVFITLLSEGALLLVAAQTGFLGGPRV
                     MSNMAVDNWLPRRFTNLSDRLVIKDGVLAIGLAALLVLLYTNASVKILVVMYSINVFL
                     TFALSQWGMVRHWVKTRPPQRFRKLPIPLLALLLTVGILLITSVLKFKEGGWVTIAVT
                     GTLVLFCLWVKQHYSDTYKTLRHLDEILTQLPLPEAAESPEKLPQKPSAILMVNGYNG
                     MGIHSFLAIHRFFPGHFKNFVFLSVGVIDSDRFKDTEEIEKFKAAVQIDLDKYVRLAN
                     RMGLYAESHMAFETDVIEGLEQLCQQVSSDWHKKVYFTGQLAFEGETFLTRFLHNQTA
                     FALQRKLLFSGLEVVILPIRVRLPQRLPG"
     gene            43396..44472
                     /locus_tag="P5079_00240"
     CDS             43396..44472
                     /locus_tag="P5079_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HRY28444.1"
                     /translation="METLAAAPLSPLITAGLSAVLLAVLLLPFVFKKIEHELEIFLFV
                     MGVVAVSISGLWSGHLVRDALLDPIRLKHPIVEAVLIAGLIFRALRTHIRGYVTALER
                     LLGTRLFFFFLVVFLGLLSSVITAIIAALVLSEVATALHFNRKTEVKLVVVTCFSIGL
                     GAVLTPVGEPLATIAVAKLQGEPFHAGFWFLLRELGWCVLAGVVALGLFSMTFHPGKE
                     TPKSHLEQDAPESLRDVFVRAGKIYLFVMALVLLGAGFQPVIDAYVISLPSGLLYWIN
                     TVSAVLDNATLTAAEISPKMALPQIRAVLMGLLIAGGMLIPGNIPNIISASKLKIGSR
                     EWAVIGLPLGFSIMAIYYVVLFHF"
     gene            44494..45711
                     /locus_tag="P5079_00245"
     CDS             44494..45711
                     /locus_tag="P5079_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRY28445.1"
                     /translation="MNQSEKISEDKSTTKTWLFISAMALVCGQWLFLRASGAEFHNVL
                     LGAFLPGIAIFGAAYVLSWGAELLQLDLPQTLALLLLALIAVLPEYAVDMYFAWMAGK
                     DPQYTAYATANMTGSNRLLIGLGWPVVLLAFWWGSRKDSITLEPGHKIEFRALLLATV
                     YCFVIPMKRSLSLVDFIILVAIFIFYLWGAIHAEVHEPELEGPTEIIARYRPAVRRFW
                     TALFFLLAGSTIFFAAEPFAEGLLKAGRTLGIEEFILVQWLAPLASESPEFIVAVIFA
                     LRGLAGAGMGTLISSKVNQWTLLVGMLPMVFALSAGHMGPMHMDERQTEEMFLTSAQS
                     FFALVLIIDFTFGWWEAALLAVLFSTQLLFPSTAVRYVYAYGYIALGLGLLVFSGRRR
                     QCLFQLFRKQRGA"
     gene            45976..47460
                     /gene="glnA"
                     /locus_tag="P5079_00250"
     CDS             45976..47460
                     /gene="glnA"
                     /locus_tag="P5079_00250"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRY28446.1"
                     /translation="MDANKTKASIKDASKSKGQHPQNAAEAVAWARKSGVKIVDYKFT
                     DLLGTMQHISYAADKLKESVFAEGIGFDGSSIRGFQSINESDMLLLPDPATAFIDPAL
                     KIPTLSFLCTVKDPVSGKGYLKDPRSIARRAEEYLLKSGIATESWWGPEAEFFVFDSA
                     RFDLGQHGSFYELDSVEGIWNTGKDNAGQPNLAYKIRNKEGYFPTPPMDTLQDFRSEA
                     ILRMIEAGLDIDLHHHEVATGGQGEIGVGVRTLTSAADQLVLYKYILKNVAREHGKTV
                     TFMPKPLFGDNGTGMHTHQSLWKNGAPLFFDKKGYAGVSQLALWYIGGLLKHAPALCA
                     ICNSTTNSYRRLVPGYEAPTNLAYSARNRSAAVRIPMYSNEPKQKRVEYRPPDAASNP
                     YLAFSAMLMAGLDGIRKKIDPGKPYEVDIFELSTNEAREIPQVPGSLDAALQALEDDH
                     AFLLEGGVFTKELIETWIEYKEKKEHDFVRLRPHPAEFHLYYDC"
     gene            47606..>48103
                     /locus_tag="P5079_00255"
     CDS             47606..>48103
                     /locus_tag="P5079_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY28447.1"
                     /translation="MFTHPKRRIGNRLVLAALSAVLALSGQVAAEGPAFSGFIDFGYN
                     YNFNGRTINTLRGFDANANSLTLQNAELVAEGRSQSNVGYRVDVDYGYDASLIHSAGF
                     TSTGNGTLQTDIQQAYITVPCPLTRGTVTVGKFVTQHGAEVIEAKDDYNITRGFLFTY
                     VIPYTH"
BASE COUNT         9163 a        15235 c        14393 g         9312 t
ORIGIN      
        1 agtgccaaat gtcaactccg gcactattta ggagaaacca actaatcagc ctatgctgcc
       61 cagaccggca ttaacatccg agagttccca ccgcttatcc cacaaggagc ataaaatgaa
      121 ttcgtttgcg ccagcccaac tacctgtcga aaatatacat ttcgacaaga aaaacccgcg
      181 cggagaatcc gaggaagtaa ttttaaaaga tcctgaattc gaaaaattgg tgtcatcaat
      241 aaaagaattc ggcatacttg agccgcttgt tgtcaaggag gaaacaaaac aaaaagacac
      301 ctatgtttta atcgatgggg aaagacgatt gcgcgccgca aaaaaaatta gcctgccaaa
      361 agcccctgct attattgccg atagcgacac caatggccgg gttttagctt atcaaattca
      421 catgttacga aaacaatggg agaaagcatc tgaaacaaaa tccatcaagt ccataattgc
      481 cgatcttatg caagaaaatc cgaagatttc agacggcgat ttaaaaaaga agctacttga
      541 aattactaaa cacaaagacc atgagatcga cgatttaatg aatctttgta aatatgacga
      601 tgttattatc gacaaggttc tgcgtgacga attagatatg agttatttaa ttcaaaatga
      661 aaagagtttt ataacccccc tgaaaaggaa ataccccgat ttagtcgcta catatacgga
      721 agataaaata agaaaaataa tggcgcagaa agcattggat agcaaactcg ttaagagccg
      781 ctatttaatg gataccttca aatatgtttt tcgagatgaa aaaaaccatc aaaaaataaa
      841 acgtattata aaacgttttt taaattcaaa agataaaagt attgaaacgg cttttaatga
      901 atatttagaa accgacaagg cccttatgaa aaagttcgga ttgggccata aatcaaagtc
      961 caaatctacc aagaataaac cggcggccgc ccatcagtta attttgaaaa cgacgccaca
     1021 gcaacagaca aaattggcag acatcgaaaa acgtttcctg cgcagtagtt tgacaagcga
     1081 agaattggcg tatattaaag aagcgtttag ctgcatgcgg gcagcatgcc taaaggcatc
     1141 tgtcgtgatg atttgggcta cggggattag ccggataatc gattttattt caaataatat
     1201 ttcgaaatat aattcggcgg ttgaccgaat gaaggagttg gccgacaaaa aatgtaaacc
     1261 atataattat acgcattatc gaaaagtttc cgacgaggac gggttaagag atggcaatga
     1321 ttatcaatta ctaaattatc tgttgtatga aaaaattatc cacatggccg attataagca
     1381 gctgaaaagc gcatatgaca cgcgatgcga ttgtgcccat cccacaaaca tcgatctaaa
     1441 agttaatcgt gttttaacca ttttcgaaga aatctgtaat cacatttttt taaataaatc
     1501 cataaaatga aaacactcct cgtagaaccc gatttcccaa taccctccaa aagcaaaaat
     1561 cactcaaatt ttttgcctgt gggattatta aaaatcgcag catatcatcg acatctcagg
     1621 gatcgcgtag agctacatcg aggaaactac ccggcattac gatatcccga tgaaataaaa
     1681 attacttcac tttttacata ttggtcggcg tatgtttggg aaagcgtccg ttactataaa
     1741 aatctttacc ccaaagccaa aattgaatta ggcggaattt atgcgtccct tatgcccgaa
     1801 catgccaaac aaacaggctg cgatacggtt gaacctggtt tatatcgcaa tggcgtggca
     1861 gaaaattata tccctgctta cgatatggtg gatgtggatt atcaaataat tcatgcttct
     1921 cgcggatgtt ttcgcagatg caatttttgc ggaacctgga aggttgaacc gctggtgtca
     1981 ttcaaaaaaa ctattcagcc cgagattaaa aaaaagaaat taatttttta tgacaacaat
     2041 ctattggcta accctcatat aaaaaatatt cttggcgaac tcgcggaatt tagattccct
     2101 aatcgctgca ctgttgaatg tgaaggccaa agcggtcttg atggaagatt gttaactagc
     2161 gatcctggaa tagccgtttt attaaaaaag gctcacttta aaaatccgcg aattgcctgg
     2221 gatgggccat acctgatgtg gccacaaatt aaagaacaaa tattctccct tcgaacagcc
     2281 gggttttcca gcgataatat ttttgtattc atgctctata accatcggct cagctatgat
     2341 gaaatgcggg ccaagttgga tgcatgtcgg agatgggggg tacgcgtaat agattgtcgg
     2401 tttcgaccat taaatagcat ccaggatgga tatcgacccc gagctaaagg ccaaaaagcg
     2461 acggactatt ttgtccacga aaactggaca gatcgccagg tgcgaacatt tcggcgtgca
     2521 gtaagaaggc aaaatattgc cattatgcta gggctcccca acggacgcta tctcgatggg
     2581 attgaagaaa aatatattca ggtttaaatg aaaagaccga agatcgacct ctcctgctaa
     2641 cggccaatag cttctgaaaa agtctcaaaa acgagtggaa atccggtgga aatccgggga
     2701 caggatactt aattcgccac ggcgttcgcc ctcggcgccg cttcaggacg tgcaagctgg
     2761 cacctgtctt gatagttggt agattcagca acgcgcccca gggaaagagg caagtctcaa
     2821 ctgccaactt cggtaacatt taataccgtt tttgcctgaa accaagcacc acaggaggac
     2881 ctgatgccga atcaagcgcc cccgtcccag aatgaccgaa cagcgctcga gaacatgcac
     2941 caactcatgc ttgaagccct ccgacacagg gagcaagaaa tttttcaata tctcgccatt
     3001 ctcggaccag cccttggtgg atttatttgg ttgtgctaca aaaaagaagc cgcctcggct
     3061 tttgttcctg ggactattgg cgtattgctt gcgcttcttc tcggtgcaat ttattcccta
     3121 gccctgggtt ataattttcg ctatatcgtt tttgaattgg caaagctcga gattatcctc
     3181 gggatgaaag atgccatgtt gctcgcgtgg cccaggagtg tcgaggcctt ccgaaaaagt
     3241 gcatttttgc accatcgctg gatgccaatt ccctgggtca cacctccgga aataataaaa
     3301 atattttgga tttcttttct tgtttttatt cttggagtaa cggcggcggc acaacgattt
     3361 aaacccatcg gcatttcgga agaacataca aaactgatta tttatgttgg cggagtttct
     3421 ttcttggcgg gaatcttaac ccctatcctc tttggatgga aatttctcga caagtgcaaa
     3481 aaagaacgcg ggtggtaacc gcccataaaa ttcaaccagg gctggtgctt gtcttaacag
     3541 ttgcgaacca tctccggctc ttccattagt ggcggttttt ttattccagg gatgttggaa
     3601 aacccgatag cacggaggta tttttatggg atcttccacg gggtctttgg cggcatccat
     3661 cgcggagttt caacagtatt tgaaggccaa agtagcggtc cacatcgaaa ccgagcataa
     3721 aaacctttat cccttcatca cgatttcccg cgaagcgggg gcgggcggac atttactggc
     3781 gcaagccatc ctgaaacgaa tggagaagga aagccttccg gtctatcaag gatggcacat
     3841 gctcgatcag gaaatttgcg aagccgtcct tgccgacccg aaaatgaaga cctcccttga
     3901 gccgctgctg tcggaaaggt tcaacagcga actgaacgat tatctgtcgc aaatcatcgg
     3961 cggcacctcg ccgcaggtgg cggtctacac gaaaatcttc cgcctcatca tgtccctggc
     4021 cagcgttggg aaagtggtcg tcgtcggccg ggccggatct ttcgtcacgc ggctgttgcc
     4081 cctgggaatc cacgtgcggc tggtggcgga gaaggaacag cgcatccaat acatgatgcg
     4141 cgaaagggac ctgccccgtg acaaagcgga aaccaccgtc gcccaactgg accaggaccg
     4201 gcaacggctg gtgaagacgt ttttcaaccg gaacgcggcc gacccgctgg aatacgacgc
     4261 cgttatcaat acgagccggg tccctctgga gagcgcggtg gacgcgctca ttgcgcttgt
     4321 gaaacaacgg tttgaaaagg cgccggccgt gttggcgtcg aaagcttgat cgaaaaccgc
     4381 gcccgttttg ccgggtgccg gtttccctga cattctccgg cccccttcga tttcagccat
     4441 cgcagccacc ctccatttga cgtccccctc aaaaattgga ctaattgaat ataatttccg
     4501 ttggggtctt ttgcgatgag ttctgaaaag cggaggaatc catgaaattt tctcaggcaa
     4561 aacaaggccg gaccttcatc atccgtttgg aagacgggga catcatccat cgggaaatcg
     4621 aaaaattcgc ccaaacacat tccgttcggg ccgggttttt ggtcgttttg ggcggggccg
     4681 acacggacag cacgctggtc gtggggccca aggccggccg aagcaagccg atcgtcccca
     4741 tgaaacacct cttggacaac gtccatgagg taaccggcac cggcaccctt tttcccaacg
     4801 aaaaaggcga gcccgtgctg cacatgcacg tggcatgcgg ccggaagaag gcgactgtga
     4861 ccggctgcgt gcgcgagggt gtgcgggtgt ggcatgtgct ggaagtgatc ttggttgaat
     4921 tgctggatac gtcggccgtg cgcgctttcg agccggacag cggcttcgaa ctgctccggc
     4981 cttaattctc ttttccccaa caccgacctc tttccaaatc atcgcgcccc gccttcgtcg
     5041 acctgtcccc gggatgtgct ataatttcag cgaaactgag aagcattgaa ttgcggaagt
     5101 tttttgacat ttttttgggg ctgcccctgc cgtaaacgct ggaaagggtg atgctttttc
     5161 cagcacgtca gggactgccc agcaacagtt gttttcatac tgatattttg aagtttttgg
     5221 ggctgtctgc ggagttgtca ccgcgatgcg gtgccgcaga tcccaaagct ctgcgtgggg
     5281 cccctgccgt aatcgctgga aagggtgatg ctttttccag cacgtcaggg actgccccac
     5341 gcgacaatgg tcgttacttt ggggctgtag ctcagctggg agagcgcgtc gttcgcaacg
     5401 acgaggtcga gggttcgatc cccttcagct ccacttaatt tgcgcctcga actgaggcga
     5461 agggtctgac cccttatggg ggtgaggccc tttttctttt tgggaaccaa ttggccgacg
     5521 gccaaaagtt tttcaattcc cgtcttgcgc gcgcacccca gcccgaaaac cgccctcagc
     5581 cacatagaaa agaagccggc cccgaccgga cggccggatg attgatataa tgattcctca
     5641 tgcggggaat tcggatacgg gaggggatca tggtcaaaag gaatacgctg aaggcttttg
     5701 tggtcttcgg tctttgttgc gggtacggtc cggccatcgg ccgcgccgac ggggccggcg
     5761 agcttcgccc ggaatggcag atcgccctcg agctctcgga aataaccgcg acggattccg
     5821 cgaccttcct ccaccatcaa tcgatgccgt ggctgtccgt gtcgcacaca ctgtcgcccc
     5881 gttggaacgt cggcgcggaa ttggcgtatc tcgacccgaa aaaagaaccc atcacgggcc
     5941 tttttaccca gaccggatcc tggtcgctga attataaatt gtccggcgga tatctcggtc
     6001 cgtatatgga ggcccttctg cagggatccc ggttcggcgc ttcttttcgt tgcggcgtcg
     6061 gggtgaccta tctccgacaa tcctgggagg gacaaggcat ttccacccag aacatgagtc
     6121 actacgaccc ttctttggtt ttttcggtgg gggggtcctg ccggatcggt tccaattccc
     6181 acctccacgt gggactaaag aacctgatag tcttccaaca cggcgaatat ctgaccacga
     6241 ccgggttccg cggcggatca ccgaacgggc cgatcttgga aaaagaagcg gtcgatacgc
     6301 ccgagcacct ttttctcccg ttcatcggag cgtccgtccg gttctagccc gccgcaaaga
     6361 tcagaaaaat aagtccacac tcccccgtcg aaggagcggg ccgggccggc gacggggccg
     6421 gagaatccgg gtcggcggat cgttggtttt ttgtattgtc agcggcgggg gcgacacggc
     6481 cggagggatg gggcgaattt ttgaaggttt cctccggatt ttttatcctg ttcctctccg
     6541 gaacagtcta ggaaaaaatc gtcccgtctt tttctgatac tcgatgtaac cttctccaaa
     6601 caggtcgacc aacattcttt cttccgatcg gatgaggaga tgccaaaggg gatgcaaaag
     6661 caccgcccaa aggaggtaaa gataactgtt caggtagacg gccaatccga aattaaatac
     6721 cgatagaaac gccgcgtaaa gcgggtggcg gacgtattta aatgccccgg tcgtgcagag
     6781 tttattgccc ctgtcggcga cgggcaacga cttgacactc caaacgatga tgaccgccgt
     6841 tattaaaatt gaaacaaaaa atgccaaact cggaaggaat cggttgtttg tgacgggggg
     6901 aaggtgaatc cgcctgttga gctggtgggc agcggacaaa agaacaaggc tcagcaatac
     6961 gccgacgggc ccgctgccaa acaacttgtc atagattttt atttttccca tcatttcatg
     7021 tcgggcctat cccttgctgg actttcagcg taacgtcgac ggaatgtcat gatacaaaat
     7081 tagccggtgt tctccatgtt ccgcatgttg gaggagaagt gaaaatccca gagcttcgtc
     7141 gattcggcgc cggcctcttc ggccgggcga tggttgtccc aaaaattatg cgccgtatcc
     7201 gggaaagaac gtttgacggc gcgggccgga ccggcgaagg gccgcgtttt tttcgccaag
     7261 agcgcgcgat gtcgtcaaaa acgccgagac gtgttttggg tggacacgtg cgggaaatac
     7321 ggaccttggt tcaccttctt ttctcgagac ccgaaccctt gatgactctt ccccttcccc
     7381 cctcatgacc cttcccctgg aacagcgact gcgggcctac gtggacccct ggctgaagga
     7441 cttcaacgtt ctgggcgtgg cggtgcacat caccggcgac cgaccgctcc cccttttcgc
     7501 cggcttcgcc gacccggaaa agaagcgccc cctgtccgtc gagtccgcct ttccggcgta
     7561 cagcgccagc aagagctaca tcgccgccgt ggtgctgcgc ctggcgcagg acaacctcct
     7621 cggcctggac gacccgctgc gccgctggct gcccgacctg ccttttgaca aaccggtgac
     7681 gctccgccag ctgctcaacc acacctccgg cctgccggat taccgcgccc tgaacagcca
     7741 caaggcgggc atggcgcaat cgccgtccgc cccgtgggac gaaccgcggt ttttggaggc
     7801 cgtgttcgcc gaaaaattcc tgttcgagcc cggcacggcc tggttgtatt ccggcacggg
     7861 ctatctcctc ctgcgcaaag ccgccgaggc ggccgcgcgc cggccgtttt cccagctgct
     7921 ctccgaacag gtgctggaac ctctccgcct gcgccgcaca tttttgctga acggcccggc
     7981 ggacatgcga cgcctcacgc ccggctacgc ccgccagttc aaccccgccg gccggccgga
     8041 ggacgtgcgc gccgttttcc atcccggatg gatcgcccac ggcgtggtgg gcgccccgct
     8101 cgaagagctg aacgttttct tcgacggtct tttgaacggc cggctgttgt cgccggactt
     8161 tgtttcggaa atgaagaaac ccgtcctcat tcccttcaaa catccctttt tcgcctcgcc
     8221 gggctacggg ctgggcctgc gctattacga cgacccgcgg ttcggcaaga tcttcggcca
     8281 cggcggcgaa gggcccggct ttgcgggctt ttcctattat gtggaagcgc ggaaaaccct
     8341 ggtgtcgctg tactgcaaca cggacgtgcg ggcacgtttc ctggagaaac tgatgtttca
     8401 actcctctcc gtccttcatg acctcctctg accgccccca ccgcgcatga cgccccgctc
     8461 ttcccgctcc tcctccgtta aagcgtatct ggccctgggc gcgggcattc tgtgcatcgg
     8521 gttctcggcc atctttgtga agttcgccgc agtgccgggg ccggtgtccg cgttttaccg
     8581 cactttcgtg gcgagcgccg cgcttctgcc gtggtggctg ggacggggcc cgcgcctgcc
     8641 cggaaggaaa gacatgcggc tcatcctgtt gggcggggtg ttctttgccg tggatctggt
     8701 gctgtggaac tccagtctgc tgctcacgcc ggcggccacc gccaccctgc tggccaacaa
     8761 cgcgccgctg tgggtggggc tgggaagtct gtggcttttc cgggagaaat tgccggggtt
     8821 gtattgggtg gggctggctt tgtccctggc cggcatgacg atcttgatcg gcgagacggc
     8881 gtggtacagc catcggctga acgccggcga cctgatggcc ctggccgcca gcgggtgcta
     8941 cgcggtgtat ctgctttcca cccagcacgc ccgcgcccac gtggacacac tgacgttcat
     9001 gacgctctcc acgctgacca gcctggcgct cctcggcgcg ggggtggcgt tgttcgggct
     9061 gtcgttttcg ggctattcgg ccaaggcgtg gatgtcgctg ttggggttgg ggctgatttc
     9121 tcatctggcg gggtggctgg ccatcaacta cgcgctgggg cacctgcggg ccgcgccgct
     9181 gtcggtgagt ctgctggggc aggccctcgt gacggcgctg ttggccatcc cgctgttcgg
     9241 cgaaacgctg cggacgcacc agctcatcgg cggcacgctg gtgctggccg gcatcttcct
     9301 ggtgaaccac cgccgcacgc gcgccgaggc cggccccacc gccccggaaa acgtgtgagg
     9361 ccgcttccta tttgatcgtg agttttttgt atttgctgcg gcgggattct tcgcgtccgc
     9421 ccagctcgcg gcggcgcact tcctggtatt cctgaaagtt cccctcaaac caacgcagtt
     9481 cgccttcccc ctcgaacacc aacaggtgcg tgcagatgcg gtcgaggaag aaccggtcgt
     9541 ggctgatgac cagcacgcag ccgctgaatt ccatgatggc gttctccagc ccgcggatgg
     9601 tgtccacgtc caggtcgttg gtgggctcgt ccagcaggag cacgttgccc cctttcagca
     9661 tctgtttggc caggtggagc cggttgcgtt cgccgccgga gagggcggcg actttcttct
     9721 gctggtccgt gcccttgaaa ttgaaccgtg acagatacac ccgcgccgga acgagtttgc
     9781 cgccgatatc gaggtggtcg accccgccgc tgatctcctg gaacaccgtc ttggtctcgt
     9841 ccagggtctc gcgtttctgg tccacgtagg acaacgacac cgacgggccg acgaccagct
     9901 cgcccttgtc cggtttttct tcgcccacga tcatgcggaa cagggtggtc ttgcccacgc
     9961 cgttggggcc caccagcccc accaccgcgc cgcgggggat gtcgaaggag acgtcctgca
    10021 ggagctggtt gtcgccgtat cccttgaaaa ggtggcgggc ctcgatgacc ttggcgccga
    10081 gcggcgcgcc gggcaccacc tggatgtcca gcgagttgac gtccacggtc ttctggtgag
    10141 acaccagcgt ctcgtattcg cggatgcgcg ccttgctctt gccgctgcgg gccgacggcg
    10201 tgaggtgcat ccaggccagc tcctccttca gccgccgctg cagggacgac gcctttttct
    10261 cctccagttc cagccgtttg gacttctgtt ccagccaggc ggagtagttg ccttcgaagg
    10321 ggaacccctc gccgcggtag agttccagga tccagcgggt gatgttgtcc aagaagtagc
    10381 gatcgtgagt gacgatgatg acgttgccgg gataatcgcg cagggtgcct tcgagccagt
    10441 tgacggtctc ggcgtcgagg tggttggtgg gctcgtccaa aaggaggatg tccggttttt
    10501 ggagcaggag tttgcagagg gccacgcggc gcgcctcgcc gccggagatc ttggtggtgg
    10561 cgaggtcgtc cggggggagc accagcgcgt ccatggccac gtccacgtgc cgcttgatgt
    10621 cccacccgtc cgccgcctcg atggtctcct gaaggcggcc catcttctcc agggtctttt
    10681 ccatggcgtc gtccgacatg ggctgggaca tgctgtcgga aagtttctca tattccgcca
    10741 ccaggtcggt gatgtgcttg aaggccgact ccacctcctg gcggacggtc ttggcggggt
    10801 cgaggcgggg ttcctggggc aggtacccga tgcgcacgcc cttcttgggc tcggccgacc
    10861 cgatgaaatc cttttccacg ccggccatga cccgcaacag ggtggacttg ccggtgccgt
    10921 tttcccccac gatgccgatc ttggcgccgt ggtagaagga caggtggatg tccttgaaga
    10981 cgaccttggg gccgtaggac ttgctgagcc ccgacaaaac gaagacgtat tcgcccgcca
    11041 tggttccgtt ctccttggga tatcggtgtc cgccaaaaaa acggtcccgc gagtttcggc
    11101 ccgcgagacc gtttttaagg acgggaggaa cttatttctt tttcgcctga ccgggcagcc
    11161 gggccttggc ggcggcgtcc accgaggtgc gggcggccgt gagctggggc tggagttcgt
    11221 ccagcttctg caaggcgccg ttgtagtcgc cgccgtcgaa agcggtctgc acgcccgccc
    11281 acaggtcggt caacgcggcg gcctccgcct tgatgggttc gaccccccgg cctttcagct
    11341 tggacgccgc ggactggacc gcgtcgatct ccggtttcag cccgttcatg cgttcctgca
    11401 cggtggtcat caccgcggcc ttgttgggct ccacggcgct ctgggccacg tcggccttgg
    11461 ccttggtttc gagggcggcc gctttggcgc ctttatagtc cttgctttcc agtttggcgc
    11521 gggcgtcggc gagggcgtcg ttggccgcgg ccagctccgt cttggcgtat tgttccgcgc
    11581 cggcctgcac ggcggcggtc accgcggctt cggcgtcgga catttcctgg gtgggcggct
    11641 tggcgcacga aacgaagaac ggcagaacca cgcacaccag tccctgcttc catgacattt
    11701 ttccgatcat acgaaacttc ctcctgattt tttgaaaagg gccggcgacc acacgcggcc
    11761 ccgattcgaa attccccggc acggccggcg cgccgcacgg aacaacggcg gaaaacgtct
    11821 tgaaaatcgg cggaccggcg gccccgaaga cgttcatcca tcatcgggac cgccgatccc
    11881 aaagaactat ttcttcttct tggatttgac cttctctttc gccttttcca gggcgggctt
    11941 cgcttcctgc acctgggtgg agatctcttc ggctttcgcc ttgatctcct tcactttcat
    12001 ttccacgccg cacttcatgc atttacccgg ggcatccgac tctttgccgc actgggggca
    12061 gctgtaaacc tgcttctcct ccgttttttt gacgaggtcc atgccgcatt tggcgcattt
    12121 gccggcgccg gtgtattccc cgccgcacat ggaacaggtg aacttctccg cggccggggc
    12181 ggcggcgggc ggttcggccg agacctttcc cacggacggc attttcatat cgcccgcgaa
    12241 caacaggccg gcggacaggc tgacgactcc cacaacgaac aaggcacgtt tcatgattcc
    12301 tcctcggttc tttttgaaaa acgacggaga cctctatttc ttcgatcgtt cccgcagttt
    12361 ggcggcgttc aattcctgga gccgcttgag attttcctgg agcccctgcc ggaacgacag
    12421 gtcctcctgg gaacggtcct ctccggagag aaaaattttc cgaaacagag aaacgacgcc
    12481 gaactggagc gcgataagga ggagggaaac ataccatcga taggaaaaag agacatagac
    12541 gatgaggccc aggaaacccg cggggaagag gagctggaag agcccgcgcc aacgcgccca
    12601 cgcgggagcg gccccgctgc gggatttgcc gaggaacgcc ccggcgaacc cggtgaacaa
    12661 caggaggaat gcgcacagga ggtgtttcac aacgattcct attttaccat ttgaagagct
    12721 cggcggcggg gtcccgcggg gaacggtcgg aacgccaggc cttctgggcg gcggcgaggt
    12781 ccgggacggg ctgacccagt tttttccggg ccgacagggc gttgcggacc ggataatcct
    12841 tgttgttgtt attgaacacc aggaacgccc gtcggcgggg gccggcctcc cggcgcaccg
    12901 ccgccaccaa ctcgtcgacc tcgccgtcgc tgtaatggta atcgtatttt tccgccaccg
    12961 tgggcgcccg gccctctttc tgcgcgcgcc agccggcctc gttgcggccg tgacagcgca
    13021 acacgaacac cggccccgtg gccgccgcca cccgcggcat ccagaccgcg tccaccgcca
    13081 cgtggctgag cccgttgtcc tccaaaaatt tcagggtgtc ctcggtgcgg ccgggaatcc
    13141 agtccggatg gcggaactcc acggccacgg ggatggacgg gaaccggcgg ggcagggcgg
    13201 cgagggcgcg gcgcccctcg gcggtgtcgg acacccaggg cgccatttga aaaagcaaat
    13261 accccagttt gccggagcgg accagcggag acacggcctc ccggaggagt tcgaacgagg
    13321 cctcccgggc ggacggtggg aaatgccggg cttcgatttc gccgcgggcg ttccggggga
    13381 cgttgaggaa atatcgccgc agggccggcg gcacggcgag cgcctgaaca gtgtggccgg
    13441 tcagggggcc ccaggccttg actccaaaaa ggaagccggg cggggtggcc tccgcccact
    13501 gggaaacggt cggcggggcg ggcagggcgt agtagctgga gttgacctcc acgaacggga
    13561 agaagcgggc gtaccaggcc agccggtcgc gcgaggtcat ggtcttgcgc ggataaaaat
    13621 cgccgtcttt cagaagggag cggtccagcc aggccgcgca gccgacggca acttcgttgc
    13681 cttccgaaaa attcatgcga tacttcccaa gatccaacca ccggatccaa cgaaaagggg
    13741 ccgggcgccg gctgcgtcat ttttcacaag atgatacaat ggaggcctat cctttcttcc
    13801 ggaggttttg accatggacg ctttagaagc tatcgcccga cgatacagcg aacggagcta
    13861 cacttccaaa cccgtgccca aagacgtgct cgagaagatc gtggacgcgg gccggagagc
    13921 cccgacggcg cggaacgtgc agccgtggga gttcgtggtg gtgaccgacg cggccgcgcg
    13981 gaaacagatc gcggggctgg cggagaacgg caaattcatc gaacaggcgc cggcgtgcgt
    14041 ggccgttttc tgcaaggaca cgaaatatta tctggaggac ggctgcaacg ccaccaccgc
    14101 cctgatgatc gccgcggccg cgctgggggt gcagtcctgc tgggtggccg gcgacaagaa
    14161 accgtacggc ggcgcggtgg cggaacggct cggcctgccc gacggctaca aactggtgtc
    14221 cctggtgcct ctcggctacg ccgcggcggc cggccggccg ccggccaaac ggccgctgga
    14281 cgaagtgatc cactgggaaa aattctaaac acgctcaggc cgcgcccgac cgcggcggcg
    14341 ttcgaggcag aagccctttt tcgataagcg tgcgcagcag gttctccgcg atgaggcggt
    14401 atccggcggt gttgggatgc cagccgtccg ccgaaagata attccgcgcc tccccccgtt
    14461 tcaaaagcct tccaaaaaca agttggttgt cgcaaagggg gacgtccagc tccgcggcga
    14521 gtcgcgccag gaacggattg gaccggcttt caaaataatt acagagcacc ggtttcaccc
    14581 cttgccgccg cgccgtacgc accatgttcc gtatatcggc gacgctgtga tggaccagga
    14641 gcgatctcat ccggcggggg atatcctcgt tatcgttgac cgccttgtac aggcgttcgc
    14701 tcccgccgcg ccaaaggatc tctccgacca gccggcaaaa gcgataaatc ctcgatcgtt
    14761 gaagaaaaaa cacgacatct tcccatgtcc atttcccgag tcgcaccacg gggacgagca
    14821 tgaaatgggg ggccggcaga ttgttccccg cctgtatgac gacgaggtcc ggccgatagg
    14881 catccaggtc cgtctttagt ttgttgagca ggtacggggt cgtggtcccc ttcagcccca
    14941 agttcacaca ggcaaaatcc atcccgggga aacgcctttc caaaaggact tccaattggg
    15001 cgggaaaagg gccggtccga agatcttccg cgccgtcgcc ggccgtggtg gattcgccga
    15061 cgaagaggat gacgaaatcc gccgtggcgg ccgtctcgga gggcggctgg agaaaatgcc
    15121 gatgggtggc gtaatagagc cggccggtga gttggaggac gatctccgcc agaaggacga
    15181 tgaggacgcc tttgccgatc cagaccggca gccggcggag gcggcgcatg ttttaggtga
    15241 gcaacaccag gttgtcgcgg tggatgactt cggcggggca agggcggccg aggaggcgtt
    15301 ccaattcgat gctttttcgg ccgcggatct ttttcgcctc ttccgaggag taggccgtca
    15361 acccgcgggc gatctccacg cccgacccgt tgagcagttt gacggtgtcg ccggcgtgga
    15421 aggtcccctc cacccgcacg atgcccgaag gcagcaggct ccgcttgttc tgcaaaatgg
    15481 ccgcctcggc gccggcgtcc agaacgaggg tccccttgac ccggcggccc agggcgatcc
    15541 agcgttggcg ggcgttgagg ctgtccgccc gggcctggaa ccgggtgccc acgccgtgcc
    15601 cctgcaatat ctcccccatc acgccgggac gttttcccga agcgatccac acctccacgc
    15661 cggaggccat ggcgagtttc gcggcacgga gcttgctgct catgccgccc acgctcttgt
    15721 ggctgccgcc cttggcgccg gccagggcct cgatctccga agtgatttgg aacacctccg
    15781 gcaggagccg ggcgtcctgt tcgcgtcccg agaggaggcc gtccacgtcc gtcagtaaaa
    15841 agagttttcc cgcccccacc ttggccgcca ccagggccgc cagcgagtcg ttgtcgccga
    15901 actgaatctc ctcggtggcc acggtgtcgt tctcgttgat gatgggaagg acgccgcgcg
    15961 agaggagggc gctgagggtg ttgcgggcgt tgaggtagcg ttcccggtcc tccatgtcgg
    16021 agcgggagag gaggacctgc gccacggtga cgcccaaacg accgaaggcc ttctcgtagg
    16081 cctccatgag gaccacctgg cccacggcgg cggcggcctg tttgtcggac agaacggtgg
    16141 ggcgggtctt ccagccgaac ttgtccatgc cggcgccgat ggcgcccgag gtgaccagca
    16201 ccacggggga accccgtttc tgaagggcga ccagttcctc ggcgagggag cggacgcggg
    16261 ccgtgttgag tccgccctga gggcggctca acatggcggt gccgacttta acgacgacgg
    16321 ggcggttccg gcttgtaggt gaactcataa tctccgatgg ccacgaggtc ccccgcggcg
    16381 atcccttttt gttcgagcgt cttctcaaca cccattttac gaaaaatttt ctgcaggcgg
    16441 gcgatggcct cgtcctgatc gaaattggtc atggccacca gccgttcgac cccttttccc
    16501 cgcacgcgga acagctcgcc ttccttctcc acccaaaaat ccggttcgag gcggtagagg
    16561 gccgacggcg cgggcacgaa gaggtcggcg tcgggggctt tgtccagcgc tttgagcacc
    16621 gcggccagga gcggcgccag gccgagcccc gtgacggcgg agatggcgtg gacccgggtc
    16681 ttcttcagcc gccgggcgaa aacggtcctt tttttctccg cgtccgtcag gtccatcttg
    16741 gtggcggcca ccacctgcgg tttctggagg agccgttccg aataggcccc caactccttg
    16801 ttgaccgcgc aaaaattttg ataggcggat tttccgtccc agccggaaac gtccaccaaa
    16861 tggatcagca cgcgcgtgcg ttcgacgtgc cggaggaatt cgtcccccag gcccttgccc
    16921 tgatgggcgc cctcgatgag gccggggatg tccgccacca cgaaggaacg ttcgccgacg
    16981 tgcgccacgc ccaggttggg cgagagcgtg gtgaaggggt aatcggcgat cttggggtgg
    17041 gccttggtga tgcgcgacag cagggtggac ttgccggcgt tggggaaacc caccagtccc
    17101 acgtccgcca aaagtttgag ttcgaggtcc aattcggcgg actcgccggt ctcccccttc
    17161 tcggcgatgc ggggggcggt gttggtgctg gtcttgaagg aggcgttgcc gcggccgccg
    17221 cggccgccgt gggcggccag gaaccgttcc cccggatttt tcaaatcgca cacgagtttt
    17281 ccctcgcgat agaccatggt gccgggcggc aggcggatga caaggtcctc gccggatcgg
    17341 ccggtcttgt tggcgctgtg tccgctgagg gcggaaccgg ccttgaagtg gggccgatag
    17401 gcgaaatcgg ccagggtggt tttgtggggg tccacctcca gccagacgtc gccgccgcgg
    17461 ccgccgttgc cgccgtcggg tccgcccatt tccacgtatt tctcccggcg gaacgacagg
    17521 cagccgtcgc cgccgtggcc gccggagacg atgatgtgcg ctttatcggt gaacataggg
    17581 cgtcggcggg ttcagcgggg gaagaaagac ggagccgtcc cctggaagga accggtcgtt
    17641 ggatgggaga gggagcgtgg gatgaaaggc cgcttaggaa gcggccggcg cggtgccggg
    17701 ataaacgctg acccggcggc ggccgccgcg gcggcccaga gtgccgcgca ggtattcgaa
    17761 gcgcaccacc ccggccgact tggcgaagag ggtgtcgtcc gaaccgatgc ccacgttgcg
    17821 gccgggggcg aaatgcgtgc cccgctgccg aacgatgacg gcgccggccg tgacgagttc
    17881 tccgccgaaa cacttcacgc cgagacgttg tccctgcgag tcgcgtccgt tggtggagga
    17941 gccttgcgat ttggtgtgtg ccatatcagt ttcccgcctg aaccgttttg atcaggactt
    18001 cggtgaggaa ttggcgatgc ccctggagtt tcttgtagcc cttcttagaa cgtttcttga
    18061 aaacgagcac tttcttgtca cgcatctgac ggaggacctg ccccaccacc tcggcgccgg
    18121 ccacggtggg ccgcccggcg cgggcctggt cgccgctctt gacgaagagg acgtcgccga
    18181 accggacctc ctcgccctgg ttctgcggga gtttttccac gcggatcttg tccccgggag
    18241 aaacggcgta ttgtttgccg ccggtgcgga tgacggcgta ggaatcgctt ttcataggcg
    18301 ggaatcatat ctcatttggg aggggtacgt caacccgtca aacggcttca ggcgctgcgg
    18361 tggacggaaa ggcgggtcgg ggccggatcg ccggggcgac ggtcgccggg gcgggagaac
    18421 gtgtcgagag actccttcca cgtctccacg tccgcccgga agacctcggc cagccggtcc
    18481 aacttctcga aatcgtcgtc gctgaggtag gtgaggaacc gcgacaccac cacgttgcga
    18541 cgcaagtccg ccaggtgttt caggacggtc tccagaacgt cgaccaggta ggatttttgg
    18601 tccttgcgca gcgcgtcgga gagaaaagcc gagatggcca gggaagactg ccgcatttca
    18661 tacaccagcc cgctgccctc ggagatgggg aaggtccgcg tggagcggta cacctcggag
    18721 gcgagaacat gcgccttctg aaaaatcgcc tgtccgtcgt tccaggaaat gtgtcccatg
    18781 gccgtccttt tcccccggaa ttttaaacct ctctccttta cagtttaggc ccgaaacctc
    18841 tttttgtcaa tggttaaatt gatttacaag tcaaaaacag ccaccgggct cgacgggagg
    18901 aagtatttca ttctcggatc tttttgcctt ccaatcgcgg aattttccat ttttatatta
    18961 aaacatgtct ataaatcaat acgtttttat attaatttta ttgtccatgt tgtaaatttt
    19021 tttaactttt acttgacaaa acgccgctcc gggtatacgc tatagtaaag agattttaca
    19081 ttaggaggac tcccatggtg gataccgcga tctcgatttt ttccgtcttt cttcaacacg
    19141 cgacgcgcct gaacgaggcg ctgcccggcg atcccacggt ccgaacggga gtggaaaccg
    19201 tcctgtgtct tttcctgatg accctccttt atagggtttg gcgggaacgg atcctggcgg
    19261 aggaggggac gtttgacggg gagctgtggg atcggatact ttccctcgtc tttcagcatc
    19321 ggaccaaaga cgccgccgcc ctggccgaaa cgacgcccgg ggtgttcgcg gaactttatc
    19381 tggcggcgtt ggattattcc gcgcaaggcc gggaagacgc ggcggacgcc gtggaatcgg
    19441 tgttgcagga acaccggcaa cgccagggcc gggcgcgcgc gctgctggcc ggcggggcgg
    19501 cggcgtgcct ggccgcggga gcggcgggga gcgcgatctc cctgatgccc gcggcggaag
    19561 ggtggtcggc ggcggggctg aacgccctga cgaatttttc gccggacctg tggccgctga
    19621 tggcggggat ctcggcggcg gcggtgacgg ggctcttccg atccggtttt acccggcggg
    19681 gagaacgtct cgccgcgcac ctgcgccaat gcggactaaa actcctcgcc accctgctgg
    19741 aatcctcgag ggacgcggtc aaaaaagaag ccgcaaaacc ggtcccggcg ccgaaagtgg
    19801 cgccggaagc cccgctcccc cttccctcgg cgccgaaggt ccttcccgcg ccgaccggac
    19861 tttccgtctg ggagaccctg acccagcacc atctttagac ctccccctcc cgaccgaggg
    19921 gcgtttcctc gcggccacaa ataaaaatcc cgccgacacc cggcgggatt tttatggaac
    19981 gcgaccgtcg gtgggacgcg ctaaacgaac ttgtagccct gtcccttgat ggagatgatg
    20041 cggcgcgaga gtttggaacc gatcttctcg cggacccgct ggatgtgcac gtcgatggtg
    20101 cgggaggtgc ctttatattc cttgccccag acgttctcga agatctcctg gcggcccagc
    20161 agtttggggg agtagaggac caggtggaac aagatggcga actccttatc ggtgaggtcg
    20221 gagacgacgg ccccttcgtg ctgaacggtg cgctggaacc agtcgagcga gagaccgtcg
    20281 cgctcgatca ttttggggaa gccgcggcgc tcgtcggcca ggcgcagctg ggtgcgcacg
    20341 cgcgccacca gttccatgac gtcgaaaggt ttggtgacgt agtcgttggc gccggtctcc
    20401 aggccgcgga cgatgtcggc cacgtcgtgg cgggcggtga tcatgatgat ggggatggac
    20461 ttggtcttct cgtccgcctt gagttggcgg cagacctcca tgccgtccat gtcgggcagc
    20521 atcaaatcga gcaggatgag gtccggcagt ttccccttgg cggccgccac cgcgtcgcgg
    20581 ccgttcatgg cctccagggt gtcgtattcc ttgaaggaca gcccggcgcg gacgaaggcc
    20641 atgacgtccg gatcgtcttc caccaccagt atttttgaag ccatgcgttc ctctttcttt
    20701 cctatttttg agcgatcgtg aaaagcaagg aggtgtcgag caccatccag aagagcgaga
    20761 ggatgagcac gatctccacc gcgatgaaga aattggtcga aaggaagtcc cgcgccgagt
    20821 tgtaggtgtc ctcgatgcgt tccagtttca cctccaccat cttggagagc tcgtcgatgt
    20881 cgaactcctt ctgcacgttg cggtagatgg tgcgcaggtg ccagtccgaa tcgatgtgcg
    20941 ggatgttggc cgacacgttg taaagcgagt ccacgatggc cagcttgtcg atgccgaacc
    21001 ccatggcctc ccgggaaaac atctgatagc gctgcggcat cacccacacc ttgtagtagg
    21061 gcggcaggcg ggagatgagc gcctgggcgc tgtcgatctc ccggtccagg aaaaagttgt
    21121 agccccgcag gttccagaag agcgcgtagg ccagccggat gagttccagg atggtgtgcg
    21181 cctcgcccga cgacaccacc gcgccgaacc gcttgatgag gatgagctcg ttctcgtagt
    21241 agccgaaatt gacgagccgt tcctgttcca gggcgaagtc ggagaggtcg tcgtagttgg
    21301 cctcccccga gacgatgcca aagagcgcct tgtggttccg cttgacgaac tcgcccgccg
    21361 acgacaccac ccccttttcg aagacgaaca ccgggaagat gtccacctcc tccaggcggc
    21421 gctcgtaggt gtggctggcg aacttccggg cgcgttcgat gagcccgtcc accagggtgc
    21481 gcgcccaggt cgagacggcc gtctttccga cggcgatggc ctccgcgcgg caggagagcg
    21541 ccgttaaaaa accgatgtcc ccctccaccg ggaacgagag ctggatgagc cccacgccga
    21601 acttgtagat ccgcgtggtg atggtggcgt ttgaaaagat ccggccctcg aaggtgagcg
    21661 gcgccgccgg acgggaggag acttctttga cgatctcccg cgcgtccttt ccctcgaaat
    21721 ccagttccag acccaggtcg aaagggatca gcagggaaca ggtggacttc acggcgcccc
    21781 ctttaccggc tcatgaggga gatgagagcc tccctcagat aggcctggtc cacggactcg
    21841 tcgaaggaca ggccggccac gtagtagcct tcctcccact ggacccgcac cacgcgcgcg
    21901 cgcaggttct ccaggcgcat ctcgtgctgc ggctcgaacg tgatgtacac cgcctggccc
    21961 accttgaggg gccaacgcga ctccagtttc acccccgaga gggacaggtc caagatgcgc
    22021 ccccaccctt ccgtggggac gccgtccggg aactgcaaaa tcaccggcag ggaggtggac
    22081 agacgcggac cggcgcgaag gtcgttcatc atttcacctt ggggagccgt tccttgatca
    22141 gcacgccgcc ggcctcggag acgatccaca cgctgccctt ccagacggtg aacccgctga
    22201 gttggaagtt ccggttatgg gcgaagacga ggggcgcgaa gggacccttc ttctcccagt
    22261 ccaccccggt gcggttgagg cgccagagcg tgttgtgttc cccgtcggcc acccagagcg
    22321 tgtcgtccac ccaggccagc cccacgggct tggcggcggg accgtcgaag atgttcttga
    22381 cgcccaggcg ggcgtcgtgt tcgtaaatct tccccgtgta ggaatcgcag gaccagaggg
    22441 cggcaccgtc ccacgccagg cccgacggcg aagggccggg gctgggcacc aaagccaccg
    22501 acgagagcgc gtcgtccagc cgatgccgcc ggagccggcc gtcggcggag gcggtgtagg
    22561 cgtagtaggg cgccagcgcc gaggccaccg gctgaaaatc ctcgaaccgg ttgacctgaa
    22621 cgatggaaag atagtcgccg cgcaccgcca cgcgggtgag gttctgttga gaccaatccg
    22681 ccacccagag ggtgtcctcg tcgccgccca cggccaccgg gtggtccgag gcgagtttga
    22741 agatctggaa aggcggcgac cagaaccgca ggacgcccag gacgaggaaa cccgccacca
    22801 tcaggaccga aaaagccgcc accaccgccg ggcggcgcca ccacaccgcc ggccgcacct
    22861 ggcgcgaggt gagcaaccgc cggcccaggc gcgacaccga ccgtttggcc gccgccagga
    22921 aatcgcccgg cgtcaccggc gacgagaggc agtcgtaggc gccttccgtc atgcgtttga
    22981 gcgccagcgc gctgttccgt tccgggagat agaggagcag cggcgtcacc ggcgcgtgga
    23041 ccttgagcgt cttgcgcagc tgttccatga cgtcgtcgct gggagggttc tcggcgaaca
    23101 ccaggaccgg gggctcgcgc tcgatggcgg cgaaagccgg gtcgaaatcc tccagcaggt
    23161 ccaccgtgac gccggcctga cgagccacgt ccttcaactg gtcccgcacc gcgggatccc
    23221 gggcgacgat caggacctgg ctcataccgg caagaccagc cgaaaagcgc tgccctcggc
    23281 gccgctgtcc acgagttcaa gccggccgtg gtgccattcg gcgtaacgtt tcgcccgggc
    23341 cagtcccagc ccgcggtgtc cgtccttggt ggtgaaaaac ggctggaaga gccggtcgcg
    23401 ctggccctgg gggatgccgc accccgtgtc gctgaccgtg atccaaagtt ctttttcgtc
    23461 cgggcttttt tccgcccgga ggcgcacccg tccgcccgcg cggggcaggg actcgaaggc
    23521 gttgtccatc agcgcctgca gaacggcggc gaggagttcg ccgtctccca tgaagacggg
    23581 gaacacctcc ggcacgatga cctccagggt gcctttctcc agtcggtccg tgtagagttt
    23641 ggccacctgg gcgtggacga tctccggcag gttcagttcc gcgcgggccg gcacgcggcg
    23701 atgggtgagc gccaggtatt cctccagcgt ctccacgccc cgtcggagtt cctgcgccac
    23761 ctcctggaat ccccggcgca gctcgtcggg ccccaccttg atgaggcgcc accactgttc
    23821 ccaggtcccg gcgccgggct tgtcctgcac ctcgagcaga tggaattttt ccagcgtcaa
    23881 ctgcagccgg cccatggccg actgcatctc tccccgcaat ttctgcgcca cgccgcgggc
    23941 gaagatctcc tcctccgccc agaggagctc ctccttctgg tgcagggcct cctccatctc
    24001 ctggagaagt ttggtctgga gggcggtcaa ctccgcctgt ttttccgagg tcatctcccg
    24061 gctcttgacc gacaacatgg actgcacctg gagcaggtcc tcgttgagtt tggtgaactg
    24121 ttcctggaga gcggactgtt cgcgggagaa atcggacatc tggctgtgcc actcgtgcga
    24181 cttctcggcc tccagttcgg cgatgcgttg ctgcgccgcg cccaattggc ggcggaggtc
    24241 ctcgttgctt tcggcgtagc tgccggcctt gcgcgtgagg gcggcggcct cgtcgcggct
    24301 ggtcagcagg tccttgcgca ggttgtggat ctcctgcgcc aacgccagca cccgggcgcg
    24361 aagttcgtgt tcacccgtgc ccaggagcga acgcaggagc tcattctcct cccggaagat
    24421 gcgttcgacg ctctcccacc ggccggccac gtcctccact ttcttctgga actgttccag
    24481 ttcccagagg acctccttga gggccagttc ccgggcgccg tcggaaatgc cttccgtggc
    24541 ccaaaggatc ttggcgagct tctccttggt ctggtggacc agggcgggcc agtcgagctt
    24601 cggcttgaga tcggccggga ggatggtcgg caaaggttct ttcttttcgt cggccacaac
    24661 actactccgg gatgaccagt tcggcgccgg ggaggaggcg gccgcggtcc acttttttcg
    24721 ggttggcgtt gaagatggtc ttccagcgtt cggggtcgtt gtaatatttg gcggcgaggg
    24781 accgcagaga gtcgccgggc tgaacgacgt gggtgcggat cttcggcttg ggtttctcgg
    24841 tggaggcggg ccgcggcgcg gtgaggcgtt ccgcccggcg gatcttttcc tcggagacgc
    24901 ggaccttgtc ttccaggtcc gcggcgcggc gttccgcctc ggaggcgcgc gtctccgcct
    24961 gcaggatgcg ttcctccacg tcccgctggc cttcggcgcg gcgcacgctc acccgggcca
    25021 gctcctcctc ggccaggcgg cgcgcctgtt ccagatcctg cacggaggtt ttcagctgcg
    25081 actccttttc ctccagctgg gccacgcggc ggtccacctc gtccgccatg tcgagggcgc
    25141 ggtcgaaata gagttcggaa aaacgcgggg cgttgaaacg gtagatgagc gtgatgcgga
    25201 actggtcgga cggctcgttg aaatcctccg tggggacggc gtaggccacg tcgatctggg
    25261 cgggcgagaa gttgaacccg gcgccgaggg cgatgtggtt ggcgctgttg gcccggaacg
    25321 agttgccgat gcgcaggcgc agcaggtcgc ggtagaaaag ccattccgcg ccgaagtgca
    25381 gcgccgtgac gttccgccgg acctccaggt cggacagcag gatcaccttt tggacgtagc
    25441 ggtatttccc tcccagacgg agttgcgggc ccggcgggtt caccccgctc gggaacaggt
    25501 cccgcaggac gaaccccagg gtgaaatctt cgttgaactg caccagcgcg ccgccgtcca
    25561 ggccgaagcc gaccttggtc tttccgacgg cgtcgtcctt ttcgtccccg ttctgctgtc
    25621 ggatcttcag cacgccgccg ccgcgcaccg gccacggcac ccggccgcgg gtggggacat
    25681 ccatcccgaa accgctgtag agatcgaaca cgtgcgcgcc cggagcgagc gattgggcca
    25741 ggaacccgcc cgccgcgccg atgggcctcc cttgaaaact ggcgggcatg ccgaaagcgc
    25801 cgtggatctc cgaggtggtg ttgagcgtgc ccacgttcag ccggccgtag ttcaggctca
    25861 tctgggaccc cgtgaggccc gtcaatccgg cggggttgta aaagagggcg ttgacgtcct
    25921 ggtcttccgc cgaataggcg ccgcccagcg ccatggcgcg ggcgccggcc gtcgggagcg
    25981 gccggttgcg tccccacaaa ggcgacaccg cgcccaggaa gagcgcgacg catccgccca
    26041 gcatgaccgt ccttgttatc attcgtctct tgagcaccgg ctcgatttcc ccacctcgga
    26101 gttcaaaaaa cctgcaaatt aaaactgtgt aaaacttttt aacacactta atgttaaatg
    26161 gaacacggta aattgtcaat ccatttttat atttttttaa cattgcacgc gcatcagaaa
    26221 ccttgacagg accgaaagtt cgcacataga atacgtttag gcttttttcg cctacctata
    26281 taggtatgtt ctccatcgaa gaccaacacg ccatccttca gcaggcccgt tcggccatcg
    26341 ccggacggct ggggatctcc gttcccgcgg cgaccttgcg gccggaactg gccgacgccg
    26401 cttacgcgct tttcgtcacc ctgcacgcca aaggagagct gcgcgggtgc atcggtctta
    26461 tgcggccgga aggaaaattc tcccgatcgc tcaccgagat ggcgctggcg gcggcgttcg
    26521 aggacccgcg gttttcaccc ctgacccaac gcgaatatcc ggacatcgag gtggaggtgt
    26581 ccgtactgtc cccccttcaa cgcgtatcgc gagcggagga gatcgaaccg ggccggcacg
    26641 gggtgatggt ccggcggggc cccctctccg gggtcttcct cccgcaggta tgggaaacgc
    26701 tgcgggaacg ggaaacgttc ctgggcaccc tttgccgtca gaaagcgggc ctgccggaaa
    26761 acgcctggcg cgagccctcc acggagttgt atgtcttcac ggtggaaatc ctccgggaag
    26821 acgggggcgc cgaatgaccc gtcggcgatt ccagattctt tttttcctcg ttctactccc
    26881 ctcctcggcc cggcccacgg agacgacacg ggcgtccctc ccctccgcgc cgggaaagat
    26941 caaggcgtgg acggaagggc tgttccgact ccagtacgtg ttggcggccg aaggcgcgtc
    27001 caccgccgcg cggacggtgc gcgtgacgct gaaaaacgac acccgctatg tgaccgccct
    27061 ttcgccccgg ttgacggagc acgaggcggt gcatcggcgc atcaacgaga tcgccgcccg
    27121 gcggatggag acggaactgg aaggatttcg gaccgccgga gactccttga aggacggcga
    27181 acgcctcttc ttggccgcgt tccgtcgaaa gatcgccgag gcccaggaac tccaccgcgc
    27241 ctgggacgcc acccacaccg tcccctaact ccccggaaaa tggcccgtcg gtcccggatg
    27301 tcgtcaaaag gggcgagacg gcgccaagaa aaccatcgcc gcgcaagaac cgccgcgtga
    27361 ctcggccgtg gggccgaacg cgggagattt tattttgagg aggaacggcg ccggggaacc
    27421 gggccgtttg ttcgaggagc gcgggcgggc gggacgtggg cgcaggtcgg ttcttcgccc
    27481 aactcgcttc cgtgctgata ataggcgcgg gcgcggcatc cgccgcagag gaccttgaag
    27541 gaacacgcgc cgcatttgcc ttccaaaaat tccggatccc tcagcttttg aaaaacaggg
    27601 gacgactccc atatctcccg gaacggctgg gtgcggacgt tcccggcccg gaggggcaga
    27661 tacccgcaag ggaaaacatc tccccggtgg gagacgaaac agacgccgac gcccgccaga
    27721 cacccccttg aaaccggggg ttccggggat cccggcgggg accccggccg tttcgatttt
    27781 tggcgggaga tccgggtgta ttgcggggcg caggtggcct tcagttccag ccggcgctcc
    27841 gaggcgcggt cgtggagcca ggagagccat gtctcggtct cctcggagga gagcatctcg
    27901 ccgtcggcga tctgcgcgcc gcagcccacc ggcacgagca tgaagaggtg gagggccttc
    27961 gcgccgagcc gcacggccag atcgtggagg gccggcagtt cctgggcgtt gcggcgggtg
    28021 accgtcgtgt tgatctggaa ccccagccct tcggccttca ggaaactgac gttcctgatc
    28081 gtcttctcga agttccccgg tccacgcagg cggtcgtgcg ccggcgccga ggccccgtcc
    28141 agggaaaaac tcacccggcc cacgccggcg ccgcggagcc ggaccgcggc ctcctcgtcg
    28201 aggagggtcc cgttgctggc caccgcggtg ggcaggccct gttcgcgggc gaacgcggcc
    28261 aggtcgtaga tgtccccgcg cagcagcggt tcgccgccgg agaagacgag gatgggccgc
    28321 ccgaccgcga cgatgtcgac gatcatgcgg cgggcggcgg cggtgtccat gtcggcctgg
    28381 gtaaaggtat cccgcacctc caaccggcgg caatggaggc accgcaaatt gcaggcggcc
    28441 gtggattccc aaaaaaccag acggagggac ggtttcagca tggctctgtt ttctcccgga
    28501 ggagcgcccg aggcgttgtc gccgccgtgc gggcgtcaag aaaactgtat aatttcccat
    28561 ggccgtttgg aaccctgaaa aatttttcgc ctccctgccg ttcttccgga acgtgccggt
    28621 ttccgaattg caaaaattcg tctggcaatc gcgccggatg cctttcaaaa aaggcgatta
    28681 cctcttccag gaaggggaac cggccgaggc ggcctggtgg gtgacggaag gattcgtcaa
    28741 gatcgccaaa agcacgccgc agggacggct gatcaccatg gagatgctga tgccgggcga
    28801 gatattcggg ccggccggcg tcatccggat gtcgcattac ccggccagcg cggtggcggt
    28861 gaccaaaggc gcggcggccc gcctgcccgg cgaggcgctg gcggccctgg cgcgggcctg
    28921 tccctccgtg gcccagggcg tgttgacgga ggtgagtcag cggatccaga gggcgcaccg
    28981 cctgcgcgcg ctggacgcgg aatccgccga taaaaaagtg gccgccgccc tcctctggct
    29041 gcgggagaag accggttccc cgctcgccgt gccccggcgg gagatcgccg agatcgccgg
    29101 cgtggctccc gaaaccgcca tccgcgtggt gcttcagttc aaggaaaaag gctggctgac
    29161 cgccaaaccg ggagccatcg cgctggtgcg gccggaacac ctgcggaaac tcgtcgacga
    29221 gggtccatga cccccgccgc ccccgaatcg ctcaccctcg gactcaccgg cgtttccctc
    29281 tccctcgtcg tttccccgca agccacgctg accttcgatt tttccggaag acccatcggt
    29341 ctctaccaaa acgggtccta ttacgccttc gggctggacg gccgcgccat cgagaagaaa
    29401 tggcggccgg aggacgcgga gagacggaaa cgatacctgc ggctcctgtc gacggacgag
    29461 tcgcgccgcc tgagggaacg cctcgtcgat atcctgcacg cctgcgaaga acggctgccg
    29521 gaactccaag ccgggaaacg cctgcaactc tggttcaacg gcaaacgggg cgtggccacg
    29581 gccaggcggg cgggacgggt cctgcggcac ctcctcgcct tcaacaccgt ccgatggcgg
    29641 acggacgcgg aacaattccg aaaactctat cccgcgctcg gcatcctgcc gccggaccag
    29701 tacctcgccc tggtggtgca ggtgaccgaa ggatgttttt ggaagcgctg cgccttctgt
    29761 gtgttccgcc ccgaaacccc ggcccgcgtg aaatcccgcg acgaaatcct ccggcacatc
    29821 gatgcggtgc gggggtattg gggccgggcg cttccccggc gctgctccat tttcctcggc
    29881 gacgccaacg ccttcgacgc cccgccggac ctcctcctct ggacctgccg gacgctggcc
    29941 gaaatattcc cgcgcctggc cgtccctcaa aaagacgggt tcggcggact ttacgccttc
    30001 ggcgaaatcg cgtccctttc aacatgggaa gacgcggacc tggcggaaat ctcccggtcc
    30061 gggttgaggc gggtctatct cggattggaa tcaggctcgg acacattgcg ccgatggagc
    30121 ggcaaaccgg gcacggcccg gcaagccctc gaaacggtcc gacgtttgaa aaacgccggg
    30181 ctggacgttg ggctgatctt cctcctcggg ctgggcggcc gggagaacgg ccgagaacac
    30241 gtcgaaacca gcgcacggtt ccttcgggag gcgccgctgg gccggaggga catcgtctat
    30301 ttttcgccgt tgcgggagga tttttcgtcg gaacacggcc cccggctgaa ggagaaccgc
    30361 tggaccaatt tgtcggagga aggccagtgg gatcagatgt tggaattgcg ccggccgctg
    30421 gacgaacggc cgccgacgga gcggcccatg tgcggtcttt acgacatccg cgaatttctc
    30481 tattgagggc ggcggacttc cgttcggcgg cccgacgcgc cgggccgccg cccatcagat
    30541 caatttttgg agagcggcga cggccttttc gatgtgccgg tgagtgttga aagggccgag
    30601 ggaaaaccgc acggtgccgt gcgggaacgt gccgatatgt cggtgggccc aaggggcgca
    30661 gtggagcccc gcccggacca tgatcccgtc gttccgccaa agcgcgcggg cggcggccag
    30721 cgggtcccgg tcccccacac gcaaggaaac gaccgccacc cggtcttccg ccgcgcgcgg
    30781 accgaaataa cgcaccgccc tcatccgccg cagttcctcc agaaaatgtt ccgtcagttc
    30841 caactcatgc cggcggatct cggaaacgcc tcgccgccgg acgtacgtcg ccgacgccaa
    30901 caggcccgcg atccccaagg cgttgtggct gccggattca tagcggtccg gcaggaacag
    30961 cggctgggtt tccacctcgg agacgctgcc ggtgccgccc tcgcgcaggg tctgcagttc
    31021 ccgtccggga cggatataaa gggcgccggt gcccaaaggt cccagcagtc ctttgtgtcc
    31081 ggggaacgcc aaaaaatcag cacggaaggc ccgcacgtcc accgggaggg ctccggccga
    31141 ctgggccgcg tcgatgagaa gggggatgcc gcgcgaccgg gcgaaacgcc ccaccgcctc
    31201 caccggctgg atcacgccgg tcacgttgga ggcatgcacg caggccacca aacgcgtccg
    31261 cggacgaagg gccgccggta atttctcaac gatcacgcgc ccctcgccgt ccacgggcat
    31321 cacatcgatc ccgacgccga cgcgtttttt cagttcgtgg agcggccgca ggacggaatt
    31381 gtgttcgtag ggggagatga gcgcccggtc gccgcgtttc caggggagcc ccttgacggc
    31441 caggctcagt ccgtcggtgg cgttcagggt gaagacgagg cgccgctcgt cggccacgtt
    31501 caggagccgc gccagggcgc gtcggccgga ggccaggatg cggccggtct cgacggcgcg
    31561 gggataaacg ccccgccccg cgctggcccc gatctttttc tggaacgaaa ccatcgccgc
    31621 caacacggcg ggtggttttg gaaacgaggt cgccgcgttg tcgaaataaa tgccgccgga
    31681 atttctcaaa tcctcgaacc tcctccgtcg tctcttcccg gtccaccgga ccgcccttcc
    31741 ggaagtttcc aattccccgg tgaccgcggc tattttacca ttttctctcc ccgcctctgg
    31801 aagggccggt gcggcctttg tataattctt gaaatgaaaa aactttccgt ggtcgttctg
    31861 ggggccgggc cgggagggga ggccgccgcc aaaaaagccg ccgcccacgg ggcggaggtg
    31921 acggtcgtcg aaaaggcgga cctgggcggg ctctgcctga accgcggttg cgtgccgacc
    31981 aagacgctgc tggaagccgg acgattgctg aaccgggccc gcgccgcgtc ggcctacctg
    32041 aaagggagcg acgggctccg ggtggaatgg gacgctcttc aaaagaagaa aacggaggtg
    32101 gtgtcccttt tccgaaaatc catcgccgac gccttcgcgc gccaaaaaat aaaactcctt
    32161 cgcggccagg cccgattcct cgacgatcac accctcgtcg tccattcgga cggggtagag
    32221 gaccgcctct cctttgaccg ggccatcgtc gccaccggat ccgtccccct cgttccgccg
    32281 cccttcccgg ccgtggaaca cgacctcttg gacagcgaca aagccctcgg cctggcccgg
    32341 gttccgaagc gcctgctgat cgtgggcggc ggggccatcg gctgcgaatt cgcctgcctc
    32401 tttcatgaat tgggaagccg cgtggtcctt ctggaaaaaa cccccgccct gcttcccggc
    32461 gaggacgccg cgctggtcca agtcctccag acctccttcg aacaacgcgg catccaaata
    32521 aaaacagcct ccaccgtgga ccatttggaa cggaaagacg gcgtttggcg gtgtcgactg
    32581 ggcggcggcg agacgatgga ggtcgacgag gtcctcctct gcgtcgggcg gcggccgatg
    32641 ctgacgggac tgggttttga aaacgcgggg ctgcggccgg agaacggccg ccttccggtc
    32701 aacgagttcc ttcagacctc ccggccgcac atctacgccg tgggcgacgt caacggacac
    32761 tccctgctgg cgcacgccgc cgcggcccaa ggcgaaacgg ccgccgacca tgcggcggga
    32821 gtgcagacag acccctacga cccgctccag gtcccgcgct gtctctacac ctggccggaa
    32881 gcggccagcg tgggtgaatg gagacacacc gccgaaacca agggattcgt cgtgaaagcg
    32941 caccggttct tctttcaggg ttcccccaaa gcctgggcca gcgacgaagc cacgggattc
    33001 cttcagctca tttcagacaa aaacaccggt cggatcctcg gggcccagat cgtcggccct
    33061 cacgccacgg aactcatcca cctcgtttcg gtggcgctcc gcgccaagct gaccacggat
    33121 caactgcgtt ccgtggtctt cgcccacccc accctcagcg aaagccttcg tggagccctt
    33181 tcgcgcggat gacccgccgc ccctccccga ccgcgggcgt tttgtgaatc ctccgtgccg
    33241 ctcttgaaaa aatatgtgtc ggccgccgcc ctccttttgt cgggggttct cgccgccgcg
    33301 gccctcgccg agtcctccct gcgcctcgtc gacacccgcc tgcacaggga catcctccgg
    33361 gaatacgacg cgcgaatcat ctatcgccgc actcagtcgt atttctccga tcggccgtct
    33421 tcccccgagg gatcccgttt cgttttcgtc tccccccgcc tgccgcttcg ttactcggcc
    33481 ggggccattg agacggcggg agggcccctg agataccgga aaaacggcct gctcgacgac
    33541 gcgcccgtcc acggcgtcgt cctcggcgat tcctattcgg aaaatctcta tcttgacgac
    33601 agcgaggggt gggtcagcct cctttcccag gcgacccgga aacgttttct caacctggct
    33661 cgaacgggaa cgggaaccga acatcacctc cgttgcgccg aactcatcct tcccgtcgcg
    33721 cgggcggacg tcgtcctcct ccagatctgt ttcaacgacg tcctcgacga caaccgccgg
    33781 gccgaccgaa cacatccctc ccatccccgc gccggcctct tcccgcgccc ggaccgtctt
    33841 cgccgtgtcg tcgcagaaac cttccccttc aaccgctccg ttttttgttt tatgttcgcc
    33901 cgctggggaa tggaattcat cggcacacgt tttttttcac cccccctcac tcccccgatc
    33961 cccgaaacct accctcccga cgagctcgcc cggacaacga acaacatcgc ggcgatcaag
    34021 ggcctctccg aacgccacgg cgcgcatttg atcgtcatcg cctttgccga tgatcatttt
    34081 cttgaaacat tgggactccc ggcctggagc caaacgcatc atgtgcccct ggtcgtcctg
    34141 aatcggtcgt cctccctttg gcaagacgtc cccccctccg cggatttttc cgcgcacctc
    34201 ttcctcacca acccccactt caacgaattg gggaaccgta aaatcgccgc ccatctccaa
    34261 cgcgtcctcc aggagaagcc ctcagcggat gccgttcaac tcccacggga cagcgggatc
    34321 ccgggatgaa cggtccccgg cagattcgtt ccatcgcgaa ggcgcccccc gaggaccggc
    34381 caaaaaaatc ctggaggccg aagctcctgc cccaccacaa acagtccatt cccgccggac
    34441 ggaaattttt tcatgtgacg cgcctcctgg aagagtcggg gctctccacc gtttgccagg
    34501 gagccctgtg ccccaaccgg gccggatgct ggtcgggagg ctcgctcgcc ttccagatac
    34561 tgggcgacct ctgcacgcgg cgatgcgcct tctgcggcga aaaaaccggg cggccccgac
    34621 cccccgaccc ctccgaaccc gaaaaactgg cggcgatggc gcggcggttg ggcctcgccc
    34681 atgtcgtcat cacgtcgccc tcccgggacg accttccgga ccacggcgcc ggccagttcg
    34741 tcgcctgttt ggaatttttg cgaaaagaaa tcccgcggac caccgtcgag cttctggtcc
    34801 ccgatttcca ggggcgtccg gatttgctaa aaagtatcat ccaaatgcga ccggacatct
    34861 tcaaccacaa tctcgaaacc gtgcgccgcc tcacgcccga agtgcgggcg cgggcgcttt
    34921 acgaccgctc cctgcaaata ctgcgctggg cggccgaagc ggggctgcgc gtcaagtccg
    34981 ggctgatggt ggggctgggc gaaacccggg aagagatccg cgaaacgttg accgacctga
    35041 aaagaaacgg cgtctccctt ctgacggtcg gccaatacct tccgccgagc ccccggcatc
    35101 gtccgctcga ccggctttat tctccggaag agttcagttc catccgagag gaggcggcgg
    35161 gccttttcga gaacgtcttg gcggggccgc tggtgcgaag ttcctaccac gccgcggaaa
    35221 tggccggcgc ccgatgaacc ggaccccgct ctaccccctg caaaaacagc tcggcgcgcg
    35281 gttcatggat ttcggcggct gggaactgcc gctccaattc aagggcatcc tcgccgaaca
    35341 caaggccgtc cgcgagaaat ccggattgtt cgacatctcc cacatgggac aggtctgggc
    35401 cgaaggcccc ggggccgagt cgtttttaaa cggccttctg acgaacgaca tcgccgcgct
    35461 cccgccccaa aaggggctct acagcgtgat gtgccgcgaa aacggcgggg tcatcgacga
    35521 tctttatgtc taccggctga cgacggaccg tttcctcctc atcgtgaacg cctcccggaa
    35581 aacggccgat ctcgactgga tccggcggca ccggccgggg ggcgtgaaca tctcggaaca
    35641 gcccgaggcc gcggccgtcg cgctgcaggg gccggattcg ccggacctcc tgaatctgct
    35701 ctgtgctccg gccctggccc tgggccggaa cggcgtcggc gctttcacca tcgaaggatc
    35761 gggatgcgtc atcggccgga cgggctatac cggggaagac gggtttgaaa tcttcgcctc
    35821 cgcggaaaac ctgcggcgta tctttcaaaa aatatgggaa ctgggcgaac cgcgcgggct
    35881 gaacctctgc ggactgggcg cccgagacac actgcgcctg gagatgggat accgcctcta
    35941 cggtcaggac ctggacgaag accattcccc cctggaggcc gggctgggat ggaccgtgcg
    36001 cctggaaaaa aaggagttca tcggccggga cgccctccgg gaagaaaaag aacgcggccc
    36061 caaacggcgt ttcatcgctt ttcgtccgac cgaaccgggg gtgccgcggc acggcgggaa
    36121 actctcggcc ggcaaccaga tcgccggtga agtcaccagc ggcaccttct ccccgtcgct
    36181 ccaggccggg atcgggatgg gctacgtgga gtcccgtttt gccgccgcgc cgctcgcggt
    36241 cgagatccac caccgcgacg tgcccgtctc gatcgtcaag ctcccgtttt atcacaaata
    36301 aatccgttcc ttaaaatcga aggagatccg ccatgaaacc cgaagaactt cgttttgtcg
    36361 aaacgcacga atggatcgcc cccgacggca cggtggggat ttcagaccat gcccagcatg
    36421 agatcacgga cgtggtcttc gtggaactgc cgaaaccggg ccgcgtcgtg aaaagcggcg
    36481 aagccgtggc cgcggtggaa tcggtgaagg ccgcgttcga catctacgcc cccgtctcgg
    36541 ggaccatcgc ggccgtgaac gagtcggtga cgaaaacgcc gggactggtg aaccaatccc
    36601 cctacgaggc cggctggctg ttcaaagtga aggccgaccg ggaagaggaa cgcaacggtc
    36661 tgatgaccca cgagacatat caaacctttt tgaaaagcga aactcactga cgattccatc
    36721 ccgactcgcg ccgaaaggtc accgctcgaa tgttcgtgcc ccacacggaa gaacaaaaga
    36781 aacagatgct cgatcagatc ggcgtccgcc ggttcgacga cctcctggcc gatctgcctt
    36841 ccgacatccg gcggccgccc ctgaatctgc cgccgtccct ctcggagatg gagatggtgc
    36901 ggcacatgga gtccttggcg gccaaggacc atctggccct gtcgttcctg ggcgcggggg
    36961 cgtacgaaca tttcattccg tcggcggtct ggtcgctggc gctgcggggc gagttcgcca
    37021 cggcctacac cccctatcag gcggaagcca gccaggggac attgcagtcc ctgtatgaat
    37081 tccaatccct catctgcgaa ctcttcggca tggacgtggc caacgcctcg ttatacgacg
    37141 gcgccagcgc cgccgcggag gcctgtcttc tggccctgcg ccacacgggc aaacggagga
    37201 tcgtgatccc ggagaccgtg caccctcaca cgcgccaggt gattcaaacc tatctggccc
    37261 acagccccgc cgacatcgtc accgtccccc ggcgggaagg caccaccgac ctgggacggc
    37321 tccaggccgc cctcgggcaa gaaacggcgg ccgtcctgtt ccaaactccg aactttttcg
    37381 ggtgtctgga ggagtcccac gaaatagcgc gcctcgccca tgccgccgga gcgctcctca
    37441 tcgcctcctc ttatccgacg gcgctgggac taatctcccc gcccggcgag tacggcgccg
    37501 acatcgccgt ggccgaaggc cagcccctgg gcctgccgct gggatacggc ggcccttatt
    37561 tggggctctt cgcctgcaaa acggaactga tgcgcaagat gcccggccgc gtcgtggggc
    37621 ggagcgtcga ccgtgacggc cgccgcgcgt tcgtcctgac cctccaggcg cgggaacaac
    37681 acatccgccg ggaaaaagcc acctccaata tctgttccaa ccagtccctg tgcgccctcg
    37741 ccgccgccat ctacatgagc ctcgttggaa aatccggcct caaagaactc gccctcctca
    37801 acctccaaaa agcccgctac gcctcgaaac ggctggcgga aaaaggattc ccgacggcgt
    37861 tcccccaacc gttcttcaac gaattcgccg tcaaatgccc gaaaccgccg gaactgatcg
    37921 ccgagcgcct ggcaaaagac aaggtgctcg ccggcttggc gctgggacgc tattttcccg
    37981 aactgaagga ccacctcctg gtctgcgtga cggaaatcaa gacccgccag gacatcgact
    38041 gtttcgccga tctattggag cgggcatgcc gatagagacc ccgcctcctt tggaaccgtt
    38101 gatcttcgag ctcagcgtcg ccggacggcg gggcctgccg tggccgaaac cggacatcgc
    38161 ggaagcggaa aacgaatccc cccttcccgc ggcgctccgg cggcggacgc ctccggaatg
    38221 gccggaggtc gcggaacccc aggtggtccg ccatttcacc cggctctccc agctgaattt
    38281 ttccgtggac acccacttct atccgctcgg ctcctgcacc atgaaacaca accccaaagc
    38341 cgcggaccgg gtggtcctcc tgccggactt catcgggctt catcccctgc ggccgcacac
    38401 cctgagccag gggatattgg aaatcctgca cgatacggaa cgctggctct gcgccatctg
    38461 cggcatggac gccttcacgc tgcagccctc ggcgggcgcc cagggggaac tgacgggggt
    38521 gctgatcgcg cggacctatc acgaatcccg cggccagaac cgccccgtcg tcctcattcc
    38581 cgactccgcc cacggcacca acccggccag cgtggcggtg gcgggtctca ccgccgtgac
    38641 catcgcctcc accccggacg gccacgtgga cgtcgacgtc ttgaaatcca aactcgggcc
    38701 ggacgtggcg ctggtgatgt tgaccatccc caacaccctg gggctgttcg aaccccgcct
    38761 ccgggagatc gcgaagatgg tgcacgacgc cggcgcgctg ctctacatgg acggcgccaa
    38821 cctcaacgcc ctggtgggcc tgttgcggcc gggggacctc ggcgtggacc tcctccacat
    38881 caacctgcac aagacgttct ccacgcccca cggcggcggc ggaccggggg ccggcccggt
    38941 gggggtcaag gcgttcctca gcgaatttct ccccacgccc cgcgtggtga aggaggacgg
    39001 cgtctaccgt cggcggatgg acttccctaa atccatcggg aaaatccacg ggttcatggg
    39061 gaacgtcggc atcgtcttgc gggcttattg ttatctccgt cttctgggag aagaaggcat
    39121 ccgccatgtc agcgagaacg cgatcctggc ggccaactac ctccgggtca aactggcgcc
    39181 gctcttcccc aagatgccga agaaaccctg catgcacgag gtggtggtgt ccggagaagg
    39241 cgctttcggc ccgggcgtgc acaccctcga tgtggccaag cggctcctgg acgaagggtt
    39301 ccatgcgccc accatctatt tcccgctgat cgtcccggaa gcgctgatga tcgaacccac
    39361 ggaaacggag accaaggaaa ccctcgacct cttctgcaag accctggaat cgatcgtgga
    39421 agaaagccgg acgtcccccg aaaaagtgac gtcggcgccg caccgcactc cgatacggcg
    39481 cgtggacgag gtcaaagccg cacgcgaacc cgacctccgc ttcaaacgca tcccttaatc
    39541 ccaccccatg tcccacgact ggcgtctcct ccacgacggc gccctctccg gcgaagagaa
    39601 catgcgccgg gaccgcgaac tcctggaaaa ccagcgccgt cccgacgccc ggccgaccct
    39661 ccggttcttc cggtggagca gccccaccct gacgtatgga aaattacaag gggaagggcc
    39721 ggcgcggcag gccgcccagg cgttcggcgc cgcccacgtc gtgcggcggc ccaccggcgg
    39781 cggcatggtc ttacacgaca agaacctcag cctttcgttg acctggccgc gccgatcccg
    39841 cgccttccct gcttcctccc ggtccctcta tcgcacggtt caccaaatcc tgaaaaccgc
    39901 cttgcgcgaa tgcgggacag aaacctcgtt ttttgtgccg gagacttcaa ggaaggccgg
    39961 accgggactc tgtttttccg aaccggtgac ggacgatctg atgtggcggg ggaaaaaagt
    40021 ggcgggaggg gcgttgcggg cgacgcggtg gggcttcctc tatgagggcg acgtgcggac
    40081 ggagctcgtg gacatcgcgc ccgagatgct gatagaaaaa atcacagcgg ctttccggaa
    40141 ctatttctca aagggctgac ccggccctca cgacatcatt tcacctacgt ccgccaaaga
    40201 accgatgcac gcagcccgcc ggctcccccc gccggcgcga caaagccaaa cagccttcaa
    40261 ccccgcccgc gtcgccccac aataatcctc ccggaaactg tcccccacgt ggatggcctc
    40321 cgaagggcgc accccggcca gtttcagggc tttttggaat atccccggat gcggcttgac
    40381 ggatcccacc acggcggagg cgaggatgaa gtccacccac ggcgcgacgc cgagcccccg
    40441 gcacaattca aaaaggcgcg agtcccaatt ggacacgatc cccaccggca cgcccctctt
    40501 tttcaatttt ttcaaagtcg gcagggcgtc cgggaaaaga cgccaggtct tcggctgagc
    40561 gaagagatcg tagagctctt caaagtaggg gcgaaacgtt tcctccgaca tacggcggcc
    40621 gaaaacggcc cgaaccaggc gggaccacca ggccttttcc tccccctcgt cggtattttt
    40681 gaggaattcc aaaccgttgc ggcggcgcca ttgggcccgg aaacgccgat ccacccattc
    40741 ggccgaggcg tcacatccgt atttccgcgc caagccggcg tagacgtggc cgacggacgg
    40801 gtggggacgg aacagtgtgc cgccggcgtc gaaaaaaacg gctttgatcc ggctcatccc
    40861 cggcgccccc tccgcgttcg acgcgccttc tcccggcgtt tcagttcgcg ccacaacacc
    40921 acgacctccg ccgccgtttt cagcctcttt cgtccgaaga catgcgggtg ccgtcggacc
    40981 agtttccggc agaggccggc caccacgtca cgaatgtcga attttcgccg ttcgctcgcc
    41041 atctgggcat ggaacaccac ctggagcaga acgtccccca actcctcgca gaggttggcg
    41101 tcgtccttcc ggcgcacggc ccgggccacc tcccccgcct cctcccgcaa atagcgcagg
    41161 agagagcggt gcgtctgccg ccggtcccag gggcatccgc cgggtccgcg cagacgggcc
    41221 atgattttta tcaagtcgtt gaaggtcccc cgtgccataa tggctcattt tagaagttat
    41281 cgcgttcccc cgccacaccc ccgcctccgt cgagcttgac agggagtcgg ctttataata
    41341 cattcctacc catgtgactc cttcgttgag agggggtctt ttttttctat gctcgaaaaa
    41401 atcaaagagc ttctttttgg taaaccgaag agtcctctgg acccgaaggt cttccacaaa
    41461 gtctccctca tcgccttctt cgcgtgggtg ggtctggggg ccgacgggct gagttcgtcg
    41521 tgttacggcc cggaagaggc tttcctgtcc ctcggtcatt tcacccacct ggccctgccg
    41581 ttggcgctga tgacggcggt gacggtcttc attctgtccg ccagctattc gcagatcatc
    41641 gaactgttcc cggcgggcgg cggcggttat ctggtggcgt cccggctgat cggccccaag
    41701 acgggactcg tctccggctg cgcgcttctg gtggactatg tgctcaccgt ggccgtctcc
    41761 atcgcctcca gcatggacgc catcttcagt ttcctccccc acgacctcct gcggtggaag
    41821 ttcctggcga cgctcctgtg tctcctcctg ttgatcatcc tcaacctgcg gggggtcaaa
    41881 gaatcggtcc tcattttaac gccgatcttc atcgttttcc tcatcacgca cctcctgatg
    41941 atcttctacg gcattttttc ccacggagcc gaacttcccg ccctgatggg agacaccgtc
    42001 caggaaacgc ggctgggcgt ggccgagctc ggttcgttcg ccatgctgtt catcctcctc
    42061 cgcgccttca gcctcggcgg cggaacgtac acgggcatcg aagcggtcag caacggcttg
    42121 gcaatcctcc gtgaaccgcg cgtcaagacc gggaagaaaa ccatgatgta catggcgatt
    42181 tccctcgctt tcacggccgg cggcctgctg gtcgcctaca tcctcaacaa cgtccatcac
    42241 atcccgggcc gcacactgaa cgccaccctc ttctacaccc tgaccgcccc atggcccctg
    42301 gggaatatca tcgttttcat cacgctgctc tccgaaggag cgctgctcct ggtggcggcg
    42361 cagacgggct ttttgggcgg cccgcgcgtc atgtccaaca tggccgtgga caactggctg
    42421 ccgcgccgct tcacgaacct gagcgaccga ctggtgatca aggacggggt tttggccatc
    42481 gggctggcgg cgctcctggt gctcctctac accaatgctt cggtgaaaat cctcgtggtc
    42541 atgtacagca tcaacgtgtt cctcacgttc gccctctccc aatggggcat ggtccgccac
    42601 tgggtcaaga cccgccctcc ccaacggttc cgaaaactcc ccatccccct cctggcgctc
    42661 ctcctcaccg tcgggatcct tctcatcacc tccgtgctca aattcaagga aggcggctgg
    42721 gtcaccatcg cggtgacggg caccctcgtc ctcttctgcc tctgggtgaa acagcattac
    42781 tcggacacct acaagaccct gcgccacctg gacgaaattt tgacgcaact ccccctgccg
    42841 gaagccgcgg aatccccgga aaaactcccc caaaaaccgt cggcgatcct gatggtgaac
    42901 ggctacaacg gcatgggcat ccattcgttc ctcgccatcc accggttctt cccggggcat
    42961 ttcaagaatt tcgtctttct gagcgtcggc gtgatcgaca gcgaccgatt caaggacacg
    43021 gaggaaatcg agaaattcaa ggccgccgtt caaatcgacc tcgacaaata cgtccgcctg
    43081 gccaaccgca tggggctcta cgcggagtcg cacatggcct ttgagacgga cgtcattgaa
    43141 ggactggaac agctctgtca acaagtgtcg tccgattggc acaaaaaagt ctatttcacg
    43201 ggccagctcg ccttcgaggg ggagacgttc ctcacccgct tcctccacaa ccagacggct
    43261 ttcgccctgc agcgcaaact cctcttcagc ggcctcgagg tggtcatcct tcccatccgc
    43321 gtgcgcctgc cgcagcgcct tccgggataa gcatggattt ctccgcgcca atggactata
    43381 atttccgcct ctgacatgga aaccctcgcg gccgcccctc tttcccccct catcaccgcc
    43441 ggactttccg ccgtgctgct ggcggtattg ctccttccgt tcgtcttcaa gaagatcgaa
    43501 cacgaactgg agatattcct cttcgtgatg ggggtcgtcg cggtcagcat ttcgggactt
    43561 tggagcggcc atctcgtccg cgacgccctg ctggacccca tccgcctcaa acaccccatc
    43621 gtggaagcgg tgctcatcgc cggactgatc ttccgcgcgc tccgcaccca tatccgaggc
    43681 tacgtcacgg cgctggaacg tcttctcggc acgcgtctgt tcttcttttt cctggtggtg
    43741 ttcctggggc tgctctccag cgtgatcacc gccatcatcg cggcactggt cttgagcgag
    43801 gtcgccacgg ccctgcattt caaccgcaag acggaagtga aacttgtggt cgtcacctgt
    43861 ttttccatcg gactgggggc ggtgctcacc ccggtgggcg aacccctggc caccatcgcg
    43921 gtggccaagc tccaggggga accgttccac gccgggttct ggttccttct gagagaactc
    43981 ggctggtgcg tcctggcggg ggtcgtggcg ttggggttgt tctccatgac gttccatccc
    44041 ggcaaggaga cgccgaagag ccacctggaa caggacgcgc cggaatccct ccgggacgtc
    44101 ttcgtccggg cgggaaagat ctatcttttc gtcatggcgc tggtcctgct cggcgccggg
    44161 ttccagccgg tcatcgacgc gtatgtgatc tccctcccga gcggcctgct ctactggatc
    44221 aacacggtct ccgccgtcct ggacaacgcc acgctgaccg ccgcggagat cagcccgaag
    44281 atggccctcc cgcagatccg cgcggtcctg atgggcctgc tcatcgccgg cggcatgctg
    44341 atccccggca acatccccaa catcatctcc gccagcaagc tgaagatcgg cagccgggag
    44401 tgggcggtca tcggactgcc gctcggcttt tcgatcatgg cgatctatta cgtggtcctt
    44461 tttcatttct agccgacgca cggaggagcc ttcatgaacc agtccgaaaa gatttccgaa
    44521 gataaaagca cgacgaagac ctggctgttc atctcggcca tggcgctcgt ctgcggccaa
    44581 tggctcttcc tccgggcctc cggcgcggag tttcacaacg tccttctcgg cgccttcctc
    44641 cccggcatcg ccatcttcgg cgccgcctac gtgctctcct ggggagcgga actcctccag
    44701 ctggaccttc cccagaccct ggccctcctg cttctggccc tgatcgcggt ccttcccgaa
    44761 tacgccgtgg acatgtattt cgcctggatg gccggcaaag acccccaata caccgcctac
    44821 gccacggcca acatgacggg ctcgaaccgg cttctcatcg gcctgggctg gccggtggtg
    44881 cttttggcct tctggtgggg ctcccgcaaa gattccatca cgctggaacc cggccacaag
    44941 atcgagttcc gcgcccttct tctggcgacc gtctactgtt tcgtcatccc catgaaacgc
    45001 tccctgtcgc tggtggattt catcatcctg gtggcaatct tcattttcta cctctggggc
    45061 gccatccacg ccgaagtcca cgagccggaa ctggagggac cgacagaaat catcgcgcgg
    45121 tatcgtccgg cggtgcggcg cttctggacc gctctcttct tcctcctggc gggatccacc
    45181 atctttttcg ccgcggaacc cttcgcggaa ggactgttga aggccggccg caccctcggc
    45241 atcgaggagt tcatcctcgt gcagtggctg gcgccattgg cgtcggaatc ccccgaattc
    45301 atcgtggcgg tcatcttcgc cctgcgcgga ctggccgggg ccggcatggg cacgctgatc
    45361 tcctccaagg tgaaccagtg gacgctcctg gtgggcatgc tgccgatggt cttcgcgctt
    45421 tcggccggac acatgggccc catgcatatg gatgaacgcc agacggagga gatgttcctc
    45481 acctcggcgc agtccttctt cgccctggtg ctgatcattg atttcacctt cggctggtgg
    45541 gaagccgccc tgttggcggt gctgttctcc acgcagctcc tcttccccag cacagcggtc
    45601 cgctacgtct acgcctacgg atacatcgcg ctgggcctgg gcctcctcgt tttctccggc
    45661 cggcggcgac agtgcctgtt ccagcttttt cgaaaacagc ggggagcttg acaaaccctc
    45721 cgattccgta gtaaagtagt tgtagcacag cgttgtgcga cagcagcttt cataagcttc
    45781 attcgtcaag gacaaagacg tcctcctcag tgactgggga ggattttttt ttgccgggac
    45841 atgaagccca ggggggacga gggggtctcg acagccgcaa ggcgtgtttt tggattctgt
    45901 cgccggccgg ccatctcgaa acgcgaccgg acggaataac tctgagggct atcctcaatc
    45961 cagaaggaga ccaccatgga cgcaaacaaa acgaaggcca gcatcaagga cgcatcgaaa
    46021 tcgaaggggc agcaccccca aaacgcggcc gaggccgtcg cctgggcccg aaaatccggc
    46081 gtgaagatcg tggactacaa gttcaccgac ctgctcggga cgatgcaaca catctcctac
    46141 gcggcggaca aactgaaaga aagcgtcttc gccgaaggca tcgggttcga cggttccagc
    46201 atccgcgggt tccagtccat caacgaatcc gacatgctcc tcctgccgga tccggccacg
    46261 gccttcatcg acccggccct caagataccc accctgtctt tcctctgcac cgtgaaggac
    46321 cccgtcagcg gcaaaggcta tctcaaggat ccccgcagca tcgcgcggag ggccgaagag
    46381 tacctgctca aatccggcat cgccaccgag agctggtggg gccccgaggc ggagtttttc
    46441 gtgttcgaca gcgcgcgttt cgacctgggc cagcacggct ctttctacga gctggattcc
    46501 gtcgaaggca tctggaacac cggcaaggac aacgcggggc agcccaacct ggcctacaag
    46561 atccgcaaca aggagggcta tttccccacg ccgcccatgg acactctgca ggatttccgt
    46621 tccgaagcca tcctgcggat gatcgaggcc ggattggaca tcgacctgca ccaccacgag
    46681 gtggccaccg gtggacaggg cgagatcggc gtgggcgttc gtacgctcac ctccgccgcc
    46741 gaccagctgg tgctctacaa gtacatcctt aaaaatgtcg cccgggaaca cggcaaaacc
    46801 gtcaccttca tgccgaaacc cctgttcggc gacaacggca ccggcatgca cacgcaccag
    46861 tccctctgga agaacggcgc ccccctcttc ttcgacaaga aggggtacgc cggcgtttcc
    46921 cagctggcgc tgtggtacat cggcggtctg ctcaaacacg ccccggcgct gtgcgccatc
    46981 tgcaactcca ccaccaactc ctaccgccgg ctcgttccgg gatacgaggc gcccaccaac
    47041 ctggcctatt cggcccggaa ccgttccgcc gcggtgcgca tccccatgta ttccaacgaa
    47101 cccaaacaaa aacgcgtgga gtaccgcccg ccggacgccg ccagcaatcc ctacctggcg
    47161 ttttccgcca tgttgatggc cgggctggac ggcatccgga aaaagatcga cccgggcaag
    47221 ccctacgagg tggacatctt cgagctctcc accaacgagg cgcgggagat cccgcaagtg
    47281 cccggctcgc tggacgccgc cctgcaggcc ctggaagacg accacgcctt cctcctggaa
    47341 gggggcgtgt tcaccaaaga gctcatcgag acttggatcg aatacaagga gaagaaggaa
    47401 cacgacttcg tgcggcttcg cccgcaccca gccgagttcc acctctacta cgactgctga
    47461 ccccgcggga tgtttccatg ccgcacgggg ccgtgcggcg aatttcgaac gaagacgttc
    47521 gtcattttgc tatagtgacg aacgtcttcg ttttttgagc gcgttacacc gactcgacga
    47581 ctactcgtct tcaaaaggag atatcatgtt cacacatccg aaacgccgca tcggaaacag
    47641 gttggtcctt gccgccctct ccgccgttct cgccctctcc gggcaggtcg ccgccgaagg
    47701 ccccgctttc agcgggttca tcgacttcgg ctacaactac aacttcaacg gccgcaccat
    47761 caacaccctg cgcgggttcg acgccaacgc caattccctc acccttcaaa acgccgaatt
    47821 ggtggccgag ggacgatcac aaagcaacgt cggctaccgg gtggatgtgg attacggcta
    47881 cgacgcctcc ctgatccatt cggcggggtt cacctccacc ggcaacggga cactgcaaac
    47941 ggacatccaa caggcctaca tcacggtgcc gtgtcccctc acccgcggga ccgtgaccgt
    48001 cgggaaattc gtcacccagc acggggccga ggtgatcgag gccaaggacg actacaacat
    48061 cacccgaggc ttcctattca cctacgtcat cccctacacc cac
//
LOCUS       DAOTFD010000002        40792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_649_length_40792_cov_4.965731, whole genome shotgun sequence.
ACCESSION   DAOTFD010000002 DAOTFD010000000
VERSION     DAOTFD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 40792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40792
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_40792_cov_4.965731"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..941)
                     /locus_tag="P5079_00260"
     CDS             complement(168..941)
                     /locus_tag="P5079_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY28448.1"
                     /translation="MRNFAVLTENVGKKFDIGTMRQKMLGQQFRYWVQGHQPTRSFWA
                     LRNVSFRVEHGEGLGVIGPNGSGKSTLLRILSGILQPNEGRVESNGPVNPFLALGVSL
                     LPQLTVMQNLKLCAALLGYTGREFRRKLDSIIEFSELSDYLYAAVADLSVGWASRVAF
                     SIAIHADLNILLVDETLEVGDAYFREKCKRFFRKIKEQGKTIILVSHSMDDIVGVCDK
                     AVYIDGGRQMAFGDVPSVITKYENTVALRMKKTAEARGT"
     gene            complement(938..1726)
                     /locus_tag="P5079_00265"
     CDS             complement(938..1726)
                     /locus_tag="P5079_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY28449.1"
                     /translation="MTYTNRLHVTPQHFWNLVKELTRCEFKLRDQSTLLGFLWTLLHP
                     TLMFVVLYALFVKWMGNHVPSFPMYLLVGVVQWNYFVKGTTGGMTSLLQKSGLISNFI
                     FPRSALVISSVFTNLLIHLLELGVMIPFLFAFGDAPSLKWFLLPGLVLLETVMILGAS
                     FLLARLAVDYKDMVRIWEIVTMTGFFVTPIFFPVEIVAPDRRQILLLNPMAQVIAETR
                     KFMVYDQWPSPRVLGILLLFSLALAWFGYRFFKKGEPHFAEKFP"
     gene            complement(1731..3047)
                     /locus_tag="P5079_00270"
     CDS             complement(1731..3047)
                     /locus_tag="P5079_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY28450.1"
                     /translation="MKKTAAPRNLVLVSVDSLRFDAPACEDDTRYLAAVDPALPARRR
                     TPVLDALARTGVHFSGAVSSSPYTTNAHAGLFTGQWPWRHGVRSLFGQEKVPAGVPLL
                     AELFKAAGFSTLSASGMPHIFHSDSFGFRRGFDDLRDISTHLFEERLSGVFRWLREHG
                     RERFFVFFHTASVHDYLTEAQWERRAGRPIADPDALYRALREPCGRALDKRLAAYLDA
                     VNFFDRNELGPLRDLVGSLGCEESTMWVILGDHGEPHPVGLEETEVRVPVIFSGNGSF
                     PRGEKIDVPVGLMDVFPTILEFFGLRPPPGLPSKSLSRCWGPRPTRPSEIYMEHFASP
                     RSSFSEPSSGPAERGTALLRGLRRGRHKLIRDCEKDEWRAYDLRRDWGERHPCDPRSR
                     KPFAPLKRRLLSIERQEGSAVESEAGRNEKPSADIVARLRQLGYLD"
     gene            complement(3049..4410)
                     /locus_tag="P5079_00275"
     CDS             complement(3049..4410)
                     /locus_tag="P5079_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY28451.1"
                     /translation="MTRVLIAGLDSIPPELFFDHPDFKMPFVRRLMSRGVYGEMVSCD
                     PPITVPAWAVLATGKEPKELGVYGFRERGENPYSDIRLASSTSIKEPAVWDYLGRRGR
                     PSVVVGVPPAYPPRRIRGPWVSCLLTPSSAPYATPPAVMKQIESWLGARPRLDAEFRR
                     EGRASTLAQIYETSRHKFEMTKRLLKNHPWDFAMFVDIGPDRMHHAFWKFFDKGHPGY
                     RPGNEFERAIPDYYGFLDRQLESLFGELPADTAVLLASDHGSKRMNGCFCVNEWLLRE
                     GYLKLKRYPEKPTPIESCDVDWPRTRAWGWGGYYARIFINVKGREPAGAVSRRDYEPL
                     RRKLARAFRGLRGPGGEGWKNQVLLSDKSWRGQPPDLLVYLDDLGHRSAGTLGHNGLY
                     LRENDTGPDDAVHARNGFFWLGGTDVPLRGRRKIRIIDIAPTVLKLLGHPLPRTLRGK
                     SIL"
     gene            complement(4491..4769)
                     /locus_tag="P5079_00280"
     CDS             complement(4491..4769)
                     /locus_tag="P5079_00280"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRY28452.1"
                     /translation="MRIGQAVGRHAKHVAWRKVDDEVVILDLETSVYYSLNETGSKIW
                     ELIGKGLSEEAIAEEVAEDYGQKVATVKKDVSAIIKKMKKENLVTPGK"
     gene            complement(4794..6065)
                     /locus_tag="P5079_00285"
     CDS             complement(4794..6065)
                     /locus_tag="P5079_00285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY28453.1"
                     /translation="MRESRRMKTKKDSRKRVLIVAPHPPFPLFESTNIRIWKLAGLLK
                     EKYAFSLATFVSEATFNDRVALSAMKLRLAMLFEKTYLVPASSENLWTDFLDKKVSEG
                     PAFERMGGVLQKALREERYDLVEIEHIQMAGCRRFVFGPPTILVEHDVGWVWFNNSLH
                     AGRRGWPVHERVRAWLKGLWSHYWTAEAFQGVVLTSPEDMRWWDTWGPSTVPTGYVPL
                     GVDLSRFSFRKVEPEMRRPELLFVGYFHHPPNADAARYFLDEIWPLILDELPDARITF
                     VGAGVPAWLKERAGKNVRVEGYVEDIVGAFKAAHVFVVPTRLGWGIKGKIVEAFAVGL
                     PVAATPSAMSGFPPETRGFVRLGRDAGEFAKETINLLKNKTLRQSMVLPARQWVEKNL
                     SWEKCAERLDAFYRRVSAPDRSYGNSGILWR"
     gene            complement(6058..7038)
                     /locus_tag="P5079_00290"
     CDS             complement(6058..7038)
                     /locus_tag="P5079_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28454.1"
                     /translation="MKLFLDFYGVRAAIESEAESILEEFRRDFQWFRADPEKSMPHVR
                     LGLSATLPPSAVPQKKNSRRFVHHDPEAVVDYDFSEEEGGVFCAEPHRLHELGYLLLL
                     SRVGEMLDRRGLHRVHGLALEWGGKCALMLLPMGGGKTTFALEALRSFPVRLVSEDTP
                     LVDEQGRLWPFPLRVGICGDRNIDGIPESMQRVFRRKEYGPKMLVDIDYWKGRIAVGG
                     PFVPSAIFVGQRGWAAPGEKLLRRVAAWRAAPALISSLLVGVGVPQGREYLLRWDVRA
                     TGGLLKNAFSRTRTMMRLLACRRVYLWRLSRDTVANAKTVSVTMETFEDA"
     gene            complement(7035..8063)
                     /locus_tag="P5079_00295"
     CDS             complement(7035..8063)
                     /locus_tag="P5079_00295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY28455.1"
                     /translation="MTNPESILLSVVVITHKRLEPLVRCIGSVEACRPPLPGEIVLVI
                     NGADAETRGWAEKHAASRPDFRWVELERSSKGRARNLGALSARGEWVYFIDDDVTVPP
                     DIFFRMREEMEAEPAAGVIGGPNSTPKDATPFQRCVGRVLTSWLGTGRMRARYRPLGR
                     RRAADDGDLILCNLMFRRALFQAGRPAFPEDFHYNEENVLLTRLKNDGVRLVYSPALE
                     VFHERRNGWGGFLRQVWHSGVGRGHMWRRVPGSFRAVCAAPSLMVVYLLTLPWVRPGA
                     IFLPLGVYLGFLGWEMARALWEDRLGVKGAGCVGLLISGAHMTYGAGLVCGFLRPGLN
                     SPSAGRDT"
     gene            complement(8145..8780)
                     /locus_tag="P5079_00300"
     CDS             complement(8145..8780)
                     /locus_tag="P5079_00300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRY28456.1"
                     /translation="MKVAGRGALRTEERTAVQTAVESLRSGGLVVFPTDTVYGLAAGA
                     FRPEAIGRIYRLKGRSYKKPLPFLVAHLDQARALVEPIPARLRRLLDRYWPGPLTVIF
                     KTSPLGRWVAGGKDTIAVRIPKHRVALELLREFGQPLATTSVNISGGTPARSGTQART
                     FCSGKVGVVLDAGRCPVGEPSTILDASTYHWVLRREGAIKEKELLKYLRGR"
     gene            complement(8848..10107)
                     /gene="argJ"
                     /locus_tag="P5079_00305"
     CDS             complement(8848..10107)
                     /gene="argJ"
                     /locus_tag="P5079_00305"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRY28457.1"
                     /translation="MAGDLPLKRLLALAKARPAALKNSALPKGFSVFGVRCGISSKPG
                     KKDMALFYSARPAAAAGVFTRNRVVAAPVLLSRRHLRTAGSVRAVIANSGCANACTGA
                     RGLSDAASSARTAARGLNVAPAQVLVASTGVIGRYLPPVPLKAGVDGLVDLVRAGRPS
                     SVGAALRAIMTTDTRPKAALERFSVRGKTVTVWGCAKGAGMIHPDVATMLVFLLTDIA
                     LPAAALRKDLREAASATFNRLTVDGDTSTNDSVFLLANGAAGVGGSSAGARRKFQEAL
                     DRVCLSLSAQIAADGEGATRTAWIFVEGAAREEDAARAAATIATSPLVKTALHGADPN
                     WGRVIAALGRAGVPFDPSTAEIRFGDVVVFRRGARAAFSEKALHKILKKDTVVIRVDL
                     RRGRGRAHYVTCDYSKDYISINADYTT"
     gene            complement(10108..11127)
                     /gene="argC"
                     /locus_tag="P5079_00310"
     CDS             complement(10108..11127)
                     /gene="argC"
                     /locus_tag="P5079_00310"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRY28458.1"
                     /translation="MINVSVVGATGYTGAELLRILLNHRHVKVKHVTSESSAGKRIDE
                     VIPSLRKKYDLVLEKPDIARLAKESDVAFFALPHGVGGRAIAEFLRHGKKAVDISADF
                     RLKDVKAYEKWYGVEHPAPDLLKKAVYGLPELWRERIRSAALVANPGCYATAATLALL
                     PLLRHKLVDEDSLIVDAKSGVSGAGKKLSSMYHFSEVTENFQAYAVAHHRHTPEVEQT
                     LSETTGRPTRITFVPHLVPMNRGILAAAYATLKKKTSAAAVRAVFENDYGREKFVRLL
                     AGEEWPQTKNVAHTNYCDINLKVDERTNRVVVLSALDNLVKGASGQAVQNMNLMFGFD
                     EGEAL"
     gene            complement(11141..11818)
                     /gene="rsmI"
                     /locus_tag="P5079_00315"
     CDS             complement(11141..11818)
                     /gene="rsmI"
                     /locus_tag="P5079_00315"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY28459.1"
                     /translation="MPLFVLATPIGNLEDVSPRALKTLSEADVVACEDTRRTWQLLAH
                     FGLRKTVVRYDNFVHRRETPRLLERLRRGERVALVTDAGTPGISDPGGRLVAEAVNAS
                     VPVVPIPGPSSVTAALSASGLPADEFSFLGFLPRRAGRVKKEFQRVGADRTVVFMEST
                     FRLKAALSAARDVFGNVPAVIGREMTKLHEEFIRGRLDDVVLELGRRETVKGEVIVVL
                     SPQLKET"
     gene            complement(11830..13086)
                     /locus_tag="P5079_00320"
     CDS             complement(11830..13086)
                     /locus_tag="P5079_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY28460.1"
                     /translation="MEEADVLLWVLVAVLALLIVALAGLRRAGRLEREQGNKYIRRLE
                     DTQEYLQQHVARLDTLVAMLASLYRLGMSGTGPANRRELCDTIVQNACRLAQTKIGSL
                     MLLTLDGETLEISASQGLSVQDAATRLRVGEGIAGRVAQTGKTIYVEDIRSDKRFLRS
                     TQSGLETQAMVSVPLRVKDRVIGVLNVNAESARFFEEREIRLLGILADQAAITLENFE
                     LYDSLRKFNIDLMQTLLQALEAKEANVQKAPSRAHEHARAIAQQLDLPEQMIQYVEYA
                     AMMRGIGKLGIVDSILRKPGKLTSEEYALVKKHPKIGSQIISPMSFLAPAAPMVLYHQ
                     EWYNGQGYPDGLAGEEIPLGARIVSVVSAWEAMTSDRPYRKALSREAAIQELKRGAGS
                     QFDPKIVDAFLRSLEAEEKRHPSAVA"
     gene            complement(13106..13197)
                     /locus_tag="P5079_00325"
     tRNA            complement(13106..13197)
                     /locus_tag="P5079_00325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13160..13162),aa:Ser,seq:gct)
     gene            complement(13821..14867)
                     /locus_tag="P5079_00330"
     CDS             complement(13821..14867)
                     /locus_tag="P5079_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28461.1"
                     /translation="MAIMGLIVPGAARALDVTLYGFVHVDTVYMDTQQMFRDNGDRVF
                     YVTGSKGENFNIHARQTRFGLKAAKEEDGYNLSGAVEMDFYGDESQVASNEISNGIRM
                     RKAFVSAQRGPHVVLAGQTADVVSPLLPDTFDFSLGLYSGNIGFWRPQVRYENSSFGP
                     FRFQASINRAYSRRENMARPDGQARIGYQSAHLDAGVSGLYGRESQTAHPLPAAGAKG
                     SVDAGALDLKVKWGSLVFSGEAYTGENIADYFGLSGFKQKEDGGWAEIKYLQTRWSAA
                     AGHARVKLRDGATIPLNTNLENRKTYGNVRYNLTKSLSTGLEYTYLEAIYKDQPEAKR
                     YFANRWNWMVHYEF"
     gene            complement(14995..16920)
                     /locus_tag="P5079_00335"
     CDS             complement(14995..16920)
                     /locus_tag="P5079_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRY28462.1"
                     /translation="MGKTSALLKIGWLIVSLVAAAALAVVVGVWRPQENVNALWLVVA
                     ALCIYALSYRFYCSFIASKVLALDSNRIPPSFRLKDGMDYQPTPRWVLFGHHFAAISG
                     AGPIIGPTLAAQFGYLPGFLWILIGATLGGAVHDMIILLASVRRNGHSLAQIVKEELG
                     VVSGMAAAFVILFNIIVAVSGSGLAVINALAGSAWGTFTVSLTIPIAIAMGFYLFKFR
                     PGKIREGSIIGALLLLAAVYAGRFIPDSSLAPFFTLSRNHLILFIGALSFLSSVLPVW
                     VLLCPRDYISTYIKIGTFLLLVVGIFIMSPTLQMPSVTRYASEPGPIIPGTVFPFLFI
                     TIACGAISGFHGLISSGTTPKMVMSEADIPLIGYGAMLTESLVAVTALIAASILIPGD
                     YFAINAPIPFDQLAALGFPVGKITEFSQQVGIELAGRPGGPVSLAVGMSAILSGILGG
                     AASMPYWYNFAIMFQALFILTLVDTGTRVGRFILQEIGGAIYKPIATHRTALNIWITS
                     FIIVFLWVYLIHAGSISTIWPMFGVANQLLAAVAFGVGTTLIIKSGKARYAWTTLIPM
                     AFMYVTTFTAAWQLIGVFSAKAARASTGAEALNLRIDQFLIVTMVLLAILILADMSYK
                     WHGYLNGTRDPKLSEVA"
     gene            complement(16913..18265)
                     /locus_tag="P5079_00340"
     CDS             complement(16913..18265)
                     /locus_tag="P5079_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY28463.1"
                     /translation="MLKTIIDISVNLTEFICVLMVVVYIMINNRLYSIDQEKITIRNG
                     ILLSLIFGIFSIYGTLAGFNIFGAYANTRDLGPALAGLVGGPFVGLGAGLIGATHRYF
                     MGGFTVIPCTVSTVLAGLGGGLVYLYKKRRIVTPFQGSIYMALFEFFHMGLVLLLARP
                     FDQAMVLVENIAFPMVFANAFGMYVYGFIIVNYRRQQETAAAKDRIEGELKAAHEIQT
                     SMLPRTFPPFPDRKEFDLYAVMYPAKEVGGDFYDFTLIDDRRLYMTIGDVSGKGVPAA
                     LFMVIAKTLLRTEALKGSSPAAILAAVNKILSPDNEACMFATVFCAVLDIRTGEMEFA
                     NAGHNPPLHAASESPFEFIDVNRAFVLGPLPEAKFVDQKLKLNPGDSFFLYTDGVTEA
                     MDPSGRQFTDDCLKETISERKGADVAAVVRNVLGDVQAFVKDAPQSDDITMLALKFNG
                     "
     gene            complement(18367..19314)
                     /locus_tag="P5079_00345"
     CDS             complement(18367..19314)
                     /locus_tag="P5079_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRY28464.1"
                     /translation="MKKLLKISLVVALALSGGTGFLGAKPKEAGLFPKMVKVPAGSFA
                     MGYIFPNGLRLGFPVHKVTLTRSFEMGKFEVTNAEFCDVLNYALEKKYLSGDYQNNVT
                     VQNAQGDSQELLDLNANFKGIKCEISFDGKAFVVEKGKEKRPLVYVTWNGAAFYCNML
                     SERAGLDALYNLTDWSCAVYGKHGYRLPTEAEWEYAATHNDGRIFPWGNDYKPEFMNF
                     GLSVGHTTDVGSYEGGKSALGLYDMAGNVEEWVNDWYSTYTPEDQTDPTGAADAVYKE
                     KRGGSWYKDDNNFAFCAYRYDTNYTYTYFFDIGFRVVKI"
     gene            complement(19381..19980)
                     /locus_tag="P5079_00350"
     CDS             complement(19381..19980)
                     /locus_tag="P5079_00350"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HRY28465.1"
                     /translation="MNDEKAAQRKLFLRFIVHQLVTTAVFIVGILACYQGAVRGIPWL
                     GPAVVALLAVIHFAGRKNRRQQMKVVLLVGGIGFLTETIMIAAGLYTPAESTRFLLPA
                     PLCPLWILALWINFALKMGDYLWFMQGRIIRGFVVGFLFGVAIFHNASVMGVGTLNYG
                     KTSLLLAAFVWGLIVPVLFRIAGKMLAAAPPAGVSVAKA"
     gene            complement(19995..21662)
                     /locus_tag="P5079_00355"
     CDS             complement(19995..21662)
                     /locus_tag="P5079_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY28466.1"
                     /translation="MNIGDVIVKTLEDIGVDTIFGGSGQSDSDILFALEKSKKIKTVI
                     IRNEQAASFMACGYAMFSNKLGVCFSTAGPGVINLFSGVAVALSDSLPILCISPYAPK
                     EYRGKGDLGETTGLHRTPDTQAMFRATTKGAFMIERPEQTCDILEEAINLAFEGRPGP
                     VNLHVDYNIMKVEVPNVRPIKLEIRPVLPLAKNVEQFADALAGLLEKKKKVVALLGYG
                     CVRSGAEKELLAFVEKFQIPFMTTMDGKGMLPEDHPLHLGMTGISGDLGAKRAFKEAD
                     AVIAIGNSFAKWSTWMFNKDLFADKLLFRINIDPHEISRYYQADYYMVSDVKPAIQKL
                     NESLGRRVSRVEKAAMPVEKKFADEKIDHKGPKIHPGQLVQEISKHLPEKSIILGDAG
                     AHMLWLAAYLKLTKGQNYQNPGSFGPMAVNVNSSIGIKCAVPERTVVAAVGDGDYQMA
                     GFELMTAVENKIPVVWVIFNNNEFNIIKMFQLRIRKAEVFNRFLNPNFKAYAEACGAL
                     GFRVEKIEDFAPAFQKALASGKPALIDVVVEPDIYPPFNPYNEPAKP"
     gene            complement(21799..22620)
                     /locus_tag="P5079_00360"
     CDS             complement(21799..22620)
                     /locus_tag="P5079_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY28467.1"
                     /translation="MTPLVEVDGQKCRGCGLCVRDCPYAALVQESKGKPPRFLEDVGC
                     LRCGHCVSVCPTGALSHAELNAEDFVPVEPSVDDDTMQKFLSSKRSVRHFLSQPVERQ
                     ALDRLLRVASQAPSDGNSQDREFVILDDKEAIQKIESAVLDGFRAFVALMKTKGMPET
                     SKEMKRSRAILDAFDKGGHPIFRGAPCVVFGCAPQKDVFSWYNVCVAMDYLMLQAHAM
                     GLGSCIVGMCLYDPPRLGKMLNLPEGHQVYSAVVLGWPAFTHRRTVSRKKPLVLK"
     gene            complement(22617..23054)
                     /locus_tag="P5079_00365"
     CDS             complement(22617..23054)
                     /locus_tag="P5079_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY28468.1"
                     /translation="MTGENRSPSAAHMEQEVLELDRQRGEALQRKDIAYLDDLYAEDW
                     SYITPFGFMVTSKKAYLEWVKNAAYETLRYDDVKARAYGNDTVVLTLKNTVEAKGAGE
                     SIGGAYLYTRVYVKENGRWKFVAQQGTLCQSAKKQEVQRNGPS"
     gene            complement(23122..23883)
                     /locus_tag="P5079_00370"
     CDS             complement(23122..23883)
                     /locus_tag="P5079_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRY28469.1"
                     /translation="MGKKVLVLATNYSVWAEELQAPWDALKKAGHQVTLATYLGKKPL
                     PIKISMDPEFVDGYQKVKVNTPEVVKRTKELLANGEWDNPIKIKDAKIEDYDALVMTG
                     GPGNPLDITNNPAVHRMILHCVKNKKVVGAICYSVGALAFTRDPNNGYKSVIYGKTVT
                     AHPRKWDFVDFDLTYDLLGASPDNKGTDLMTPGFAIPLQDIVEDAVGPKGKCISDDMA
                     TREKPCVAYDHPFVTGLSVESSIAYGKKLVEVLAK"
     gene            complement(23942..24289)
                     /locus_tag="P5079_00375"
     CDS             complement(23942..24289)
                     /locus_tag="P5079_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY28470.1"
                     /translation="MADDIYRHHESIEKKEAFKGVWGQYLGGGRRMNVMHWNFVDGVS
                     LPEHSHESEQFGYIIKGGFKVKIGARMEILKAGDAYFVPANEPHSFTSVGETEAIDVF
                     SPLREVKGYTDKV"
     gene            complement(24319..24888)
                     /locus_tag="P5079_00380"
     CDS             complement(24319..24888)
                     /locus_tag="P5079_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY28471.1"
                     /translation="MASVKKIGILLENRFIDQEIVYYSNRFKEEGMDVEFLTRLWGQP
                     RLTFKGLELGMSMTVEKSFENMDDATLRSYAAIIVPAGMVADMLRYAEKPGDLAPAVQ
                     FMKRVMAEKKILKGLICHALWIFDPIPEVIRGRKVTCHNNVIGSVRNTGAQYVDQDIV
                     RDDDLVTARTGGLFAPFARKIIDLIAPRG"
     gene            complement(24902..25309)
                     /locus_tag="P5079_00385"
     CDS             complement(24902..25309)
                     /locus_tag="P5079_00385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY28472.1"
                     /translation="MERKTFPAVLDNLEGMIAFVLEHAGKVGFSKEQMGELRLASEEA
                     LVNVVRYAYPSGPGSVDVACGPVEDGRGICIEISDSGIPFDPLSLPAPDTNLSMEQRK
                     IGGLGIFMIRKIMDKVLYRREGARNVLTLYKNK"
     gene            complement(25333..25653)
                     /locus_tag="P5079_00390"
     CDS             complement(25333..25653)
                     /locus_tag="P5079_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY28473.1"
                     /translation="MESSYSMECKDGTAKIVLTGRLDAASAPKFQEKLRELSAQKISN
                     LVFFAKDLEYISSAGLRVIIFAKQKLGADAKLYLIGAPAAVLNVIKMSGLDNFMIVQD
                     QYPG"
     gene            complement(25670..27790)
                     /gene="glgX"
                     /locus_tag="P5079_00395"
     CDS             complement(25670..27790)
                     /gene="glgX"
                     /locus_tag="P5079_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRY28474.1"
                     /translation="MHRIDSHPTHKHAKFDLRVGKPLPFGATIVPGGVNFSVFSRHAA
                     SCELVLFRKHEPQPYAVIPFPDEFRIGNVFTMVVFDLDYEELEYGFRMDGPFRPQEGH
                     RFDKTKILMDPYAKAIGGRDVWGKIPDWNNVYPHRARIVYDDFDWEGDHPLENPIEDL
                     IIYEMHVRGFTRHPSSGVKHPGTFASIREKIPYLKNLGVNCVELMPIHEFDEFENSRI
                     SPVNGERLMNYWGYSNVGFFAPKAGFAATGKFGMQVDEVKALIKELHKNGIEVILDVV
                     FNHTAEGNERGPYISYRGIDNKTYYILTPEGYYYNFSGCGNTLNCNNPIVRNMILDCL
                     RYWAADYHVDGFRFDLASILGRDQNGAPMHSPPLLETLAFDPILGKCKLIAEAWDAGG
                     LYQVGSFPSWGRWAEWNGKFRDDVRKFLKGDEGALGGMAQRLQGSPDLYQSENRGSRA
                     SINFITAHDGFTLMDLVSYNGKHNEANGENNSDGGSDNLSWNCGWEGPSTDEGVRRLR
                     AKQIKNAAALLLASHGTPMILAGDEMGNTQGGNNNAYCQDNELSWIDWRLLKENDELF
                     QFFRKMIAFRKEHPALRCIDLLNRGNSSGYPCLSWHGQKAWNCDWSAESRCLAMMLCE
                     SGALAGRPNDMFLYVAMNMHWDMHGFELPVLPGGLAWHVFANTDMRPPHDIWEVGQEK
                     AAENQGEFLVGPRSIVLLTGKPRT"
     gene            complement(27795..28133)
                     /locus_tag="P5079_00400"
     CDS             complement(27795..28133)
                     /locus_tag="P5079_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY28475.1"
                     /translation="MELKIETRDGVSVITVEGKLDGNTSAQAQEKIMPLVLPGARLVM
                     DMSRCDYVSSAGLRVLLLVAKQTASRGGKVALAGVGDEVKDVMEMTGFGGFFKNHPSA
                     DEAVRALKAG"
     gene            complement(28144..29964)
                     /locus_tag="P5079_00405"
     CDS             complement(28144..29964)
                     /locus_tag="P5079_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRY28476.1"
                     /translation="MERMNFEFSDAIAGVVTAFDKAKGVFGLKTYDGREFSVKLTPST
                     YARGPRNLDEAYVDFTGQLHDHLVPGRYLYAYGVFYPQKGTHVFEAKTIDFPGKSVDE
                     YRFEEPDWWIKQATSIADFFIRAQFLGKEIDYRNYRTMLSLSGARKGDHRQETDTISR
                     LVYGFASTFLLTGEDRFLEAAEKGTAYLRDHMRFYDTDENVVYWYHGIDVKGDGEHKI
                     FASEFGDDYDAIPMYEQIYALAGPTQTYRVTGDPLILKDIKMTVDLFDRFFLDKQRDG
                     YFSHIDPITLDPRSESLGRNKARKNWNSVGDHAPAYLVNLYLATGDNRYKDFLAYTAD
                     CIVNHFPDYGHSPFVNERFMEDWSRDLTWGWQQDQAVIGHNLKIAWNLMRINSAKPDK
                     KYVELSKKIAELMPKVGMDVQRGGWYDVVERELRGDEEVHRFCWHDRKAWWQQEQGIL
                     AYMIMFGVLKNPEYLKLARESAAFYNTFFLDHDDGSVYFNVLNHGIPYLMGNERLKGC
                     HSMSGYHSIELCYLSQVYTNLLSTGQPLELYFKPVVNGFADSVLRVQPDILPPGSVRL
                     ADVWIDGNPWKKFDAEKLTVELPTLNYRPKIKVRMVPTGK"
     gene            complement(30149..33283)
                     /locus_tag="P5079_00410"
     CDS             complement(30149..33283)
                     /locus_tag="P5079_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY28477.1"
                     /translation="MILSDLSIKKPVFAWMLMISLLLFGGIAFQRMGVSQNPDIDLPN
                     VNISLSWEGAAPEVMENEIVDQVEEAIMSVEGIRKISSTSRYGFANVTAEFDLSKNID
                     VAVQEVQTKLAAAQYNMPRDMDPPSIQKVNPEDQPILFVGVSGTRSYLEISQYVQDHL
                     KDRFQTIPGVGEVSLQGFIEPNLRVWLDPSKMHNLQLTVDDAVSAIQAQHSELPAGVL
                     QTSQAERNVRVMGEAATAEEFSRISIPTRAGQGFLWKMIRLQDIAEVKEGLADVRRLA
                     RADGKTAVGLGIRKQRGANAVEVADRVKKKIADIRKDLPPGIDISVNFDSTTFIKENI
                     HELIVTLVLAVLLTSLVCWLFLGAWTSTLNVFLAIPTALGGTVMVIYFLGFTLNTITL
                     MAISLVIGIVVDDAIVVLENIVRHREEGETRVRAAIYGAREITFSVIVISSAVIAIFL
                     PVAFMKGIIGKYFYQFGVTISVAVAFSLLEAITIAPMRCSRFLEVGHDTWMGRKMDVF
                     MARLTNGYHRLLTFSLNHRWKVILGAAFVFLVSLGLAGKLRFEMLPSQDQGVFICRLQ
                     TLPGSSLAFTDNVYKEMEKFLLSRPEVERFLGSVGGGSGGQVNTALVYLTMKDREKRP
                     IDPQKGRPLTQQEFMQVVRKEAGAIPGMRRVAIQDLSQAMGGGRGFPLEFSVIGPEWD
                     KLGEYAEAIRDRLLKSGLAMDVDTSYQVGVPEVRVVPDRQAAAERGVTVTAIARTINS
                     SVGGVRVGKYTKGGRRYDVRVSLTDEFRTKTNDISRLFVRNVRGELIPLSAVTKVLEK
                     PTLLAISRENRERAVAVFGNVAPGKSQAMAMTAAQKIAKEVLPEGYRMVPAGSSEQFK
                     EAFQDLVFALLLGIAIAYMILGAQFNSFIHPFTILLALPFSISGALGALWLSNHSLNM
                     MSMIGILLLMGIVKKNSILLVDFTNVRRLAGKGVRDALLEACPLRLRPILMTSLAIIA
                     GAVPSALTLGPGAELRAPMGVAVIGGVFLSTLLTLFVVPCAYSLVVRFESEKEASLTQ
                     EALQALGETMESPS"
     gene            complement(33280..34533)
                     /locus_tag="P5079_00415"
     CDS             complement(33280..34533)
                     /locus_tag="P5079_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28478.1"
                     /translation="MNLVIVGLMAVLPTLTMAQESGTDAVPLTTLRDVYVSALANSEK
                     IAISEQSVSQAEALYRQTRGANLPDLSFQHDTFWQRRKDTQHTGAFQIQKYGLTGYRE
                     LAALRAGRHSIAQAKYDKQRVEQLLLVDVAGAFYGLVEARQNVASTERLIALANDRLN
                     ELRERVRVGRSRNAEPIQQDYQITALQSQGEEHSRSVGAYVDFLTYLSGRPVQPPPAT
                     EVSTQTFVRSLESYLSRLHSRPDVQSAEAAVSAAKGVVDFRRGDYLPKIDLGLNLYSY
                     RPKFYKETKWDASLGVNVPLWAWGARTGAVDSALAAFRVQEQTLRFVLRQADLEVRNA
                     YRDVLSAQKQCELQAKAVDLARRDYGIQLRDDRQGLVTSLEVLESLNRLNLAELSLTN
                     FQVQSRLNAIKLEIAAGARPEEALK"
     gene            35019..36617
                     /locus_tag="P5079_00420"
     CDS             35019..36617
                     /locus_tag="P5079_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28479.1"
                     /translation="MHPSLPQTPPPATKPWTSHLLPLLVPLLAFSLFGWWRIDLPGLH
                     GEEIGPMGPLLGWMVHGPFRFLFAGYHGPVEGLCSMPFVFVLGSSVGAVRLMPLLAAH
                     GTIAALYGLAFRLSRNRGTAFAAALLFALAPTFVAGTRVGLQSGAILLFFTLGAFFFG
                     HAWLTSGRWRDLLLTAGFLALGAGCRVWAVFFDASLFLALLLTLPRSLFFFRKNARPL
                     AAAGILFAAVAGTPYLIEYARGADVLAAVTQRQAEHPSGFQKNAAACFRNIHRLIDGT
                     GYFYDMASPPMKDALNRQKIRLAGPAIFWSTLLFVVLKSLFHRPSVPPVLQFLAALIV
                     FHFSGNFLVSHPRTDYLFVLLPFMFLFLAVFWGRSFPARGKRRHLPLLLLSFFFVSRS
                     LSVLRTYTTHLAETGGWAEYSTTLTGLGETVRCLGFRQVFLPEGERPAGNTLRFLSAP
                     HGQVQYIPAAAAVSERFLRAGPTTAFVGVHSWRSRRPGSIASLIEKTAEQQGYEKMSF
                     FSFDDEPLADLFYKPIPARKKPSR"
     gene            36768..37544
                     /locus_tag="P5079_00425"
     CDS             36768..37544
                     /locus_tag="P5079_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY28480.1"
                     /translation="MRSDYYPKLLSARRTLSATEKSRLLETFGLRVPMFHRLPKQLAA
                     ELAEASGVRAYAAGEFLCRPGDPARELWVLLKGRISIHRTSWNGHRVGIEILLPGDVF
                     GLSAMLFGTYPNEILAARESVVASIPKTVMMKAIERCPELAREILLVFGQRLRFMETQ
                     LVLSRESAEKRIVAALLYLCHKFGPRLPLTRVELSEMSGTSPETTIRVLQRLTRRGGL
                     RKAPGTLLCHVRTLKTMLEAEENPGPSWDVPDGPPSSVPR"
     gene            37589..38932
                     /locus_tag="P5079_00430"
     CDS             37589..38932
                     /locus_tag="P5079_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY28481.1"
                     /translation="MRNTCFFVFGGIVLLAGLVGRAAARAEESSAASLEERYRQLEQK
                     QKILEQRLDNADARAKETILVTADPKNGFSLKSPNGAFALKFTGYAQVDYRAYVDDDA
                     KAFGDQFLLRKVRPTLDGVLHKSVDFRIQPDFGGGTTAVQDAYVDLHYSPLARVKAGK
                     FKFPLVLEALQNDTAGHFIERAYPSALTPSRDVGVQWSGDFTKTALTYAVGVFDGGAD
                     GTSPNGDGADNKDFVGRVFLHPFKNSYGSLLKQLGVGAAGSFGKEKGTAAAPGVASYK
                     DTAQQTFFSYRSDVVADGKRVRFSPQMYWYPGPLGILAEYANSSQEVKRATTTITRAQ
                     LIHRAWQVAVSCVLTGESSSYKGVKPRTVFDPAKKTWGALELVGRYTELTLDRDAFPR
                     YAEPAKYAQRARTWTGGFNWYLNDNVKFSSDYDYTRFTGNRVPNRSVFCRLQLAY"
     gene            38956..40002
                     /locus_tag="P5079_00435"
     CDS             38956..40002
                     /locus_tag="P5079_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY28482.1"
                     /translation="MFKKIVPPLSLSKRNILSLALLPLLLGGTATGAEPVIKVGVSPT
                     TSSSALYIALEQGYFKEQGLNVKLVTFPSSGAPMTIPLSQNQLSVGGGNLSAGLWNAI
                     QQNLGLKLVADKGSVSKEHDYIGLLVRKDHVDSGRYKSLQDLKGFTLGLTALNGVSQE
                     IVTELFLKKGGLTPNDIRLVKMSYAEMNVALRTKNLDATVNLEPYLTQAVLDGFAVRV
                     AGSYDVYPDQQSAAIIYSPGFAQDRTGAAEKFMVAYLKGARDYEDAFTKGKNTARIIS
                     LLQKHLQISPEAVWEKLIPAGINPDGRLNLASLERDLAWYKDKGYLTDMPDLQKAVDH
                     RFVEKALETLGPYK"
     gene            39999..40712
                     /locus_tag="P5079_00440"
     CDS             39999..40712
                     /locus_tag="P5079_00440"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY28483.1"
                     /translation="MILEVAGLTKSFDERPVLDGISLQVEAGEFVVLIGPNGCGKTVF
                     LKILSGLIPPSSGRLRLAGPTTMAFQRSPLFPWMTVRENLLISLNDPSFRGRAEREVA
                     AWLEESNLSRWADFYPHQISGGMRQKVNVIRSLMSNPPLVLMDEPFASLDYLERRRLQ
                     TFVSRLQLAKKTSLVFVTHDIPEAIFLADRILVMSGNGKIETDLRCPLPRPREHETLP
                     REAAYPDLYEKLSKLLSGN"
BASE COUNT         8190 a        13024 c        11536 g         8042 t
ORIGIN      
        1 accgccggcc agcgccgcca ggacgacgag gtcccccgtc cgcaggacct ccccctcgca
       61 acgctgaaaa cggatggcgt ccccctcccg cagcagcggc gtcatcgagg gccccttcac
      121 ctggaaccaa tcctccgccc cggccggcgg cgatcccgga gaggcgctca tgttccgcgg
      181 gcctccgccg tttttttcat gcgcagggcc acggtgtttt cgtatttcgt tatcaccgag
      241 gggacatccc cgaacgccat ctggcggccg ccgtcgatgt agaccgcctt gtcgcatacg
      301 cccacgatat cgtccatgga atgcgacacc aaaatgatcg ttttcccctg ctccttgatc
      361 tttctgaaaa aacgtttgca cttctcccga aagtaggcgt cccccacctc gagcgtctca
      421 tccaccagca gaatgttcag gtccgcgtgg atggcgatgg agaaggccac ccgcgacgcc
      481 cagcccaccg acagatcggc caccgccgcg tacaggtagt cggagagctc ggagaactcg
      541 atgatgctgt cgagtttccg tcggaattcc cgccccgtat accccaacag ggccgcgcaa
      601 agtttcaggt tctgcatgac cgtcaactgc ggcagcaggc tgacccccaa cgccaaaaag
      661 gggttcaccg gcccgttgga ctccacccgc ccctcgttgg gctggagtat gcccgacagt
      721 atccgcaaaa gagtgctctt gccggaaccg ttcggaccga tgacgcccag cccttccccg
      781 tgttccaccc ggaaagagac gttccgcagc gcccagaaac tgcgcgtggg ctgatgcccc
      841 tggacccaat agcggaactg ctggccgagc atcttctggc gcatggtgcc gatgtcgaac
      901 ttcttcccga cgttttctgt caggaccgcg aagttcctca tgggaatttt tccgcgaaat
      961 gcggttcccc tttcttgaag aaccggtagc cgaaccacgc cagcgcaagc gagaacaaca
     1021 gcaatatccc cagaacgcgc ggagaaggcc attggtcata gaccatgaac ttcctggtct
     1081 ctgcgatgac ctgggccatg gggttgagca ggagtatctg ccgccggtcc ggcgcgacga
     1141 tttccaccgg gaaaaaaatg ggggtgacga aaaagcccgt catggtcacg atctcccaga
     1201 tgcgcaccat gtccttgtag tccaccgcca ggcgcgccag gaggaacgac gcccccagga
     1261 tcatcaccgt ctccagcaac accagtcccg gcagcaaaaa ccacttcaac gagggcgcgt
     1321 ccccgaaggc gaacaggaac gggatcatca ccccgagttc caggagatgg atcagcaggt
     1381 tcgtaaagac cgacgagatc accaacgccg acctcggaaa aatgaagttc gagatcagcc
     1441 ccgatttttg cagcagcgac gtcatccccc cggtcgtccc cttcacaaaa tagttccact
     1501 gcaccacccc caccagcagg tacatgggaa aggacgggac gtggttcccc atccacttga
     1561 cgaacagggc gtagaggacg acgaacatga gcgtcggatg gagcagcgtc caaaggaacc
     1621 ccaacagggt gctctggtcc cgcaacttga actcgcaacg cgtcagttcc ttcacgaggt
     1681 tccaaaaatg ctggggtgtc acgtgcagac ggtttgtgta ggtcatctcc ctaatccagg
     1741 tagcccaact gcctcagccg ggccacgatg tcggccgagg gtttttcatt acggccggcc
     1801 tctgattcta ccgcagaacc ttcctgcctt tcaatcgata acaaccgcct cttcagcggc
     1861 gcgaacggtt tccgcgaccg ggggtcacag ggatgccgtt ctccccaatc ccggcggagg
     1921 tcgtaggccc tccattcgtc tttctcgcag tcgcggatca atttatgccg gccccggcgg
     1981 agcccccgca ggagggcggt ccctctctcc gccggtccgg aagacggttc cgaaaaagac
     2041 gaccgcgggg aggcgaaatg ttccatgtag atttcggagg gccgcgtcgg gcgcggcccc
     2101 cagcagcggg acaggctctt cgacggaaga ccggggggcg gtctcaggcc gaaaaattcc
     2161 aggatcgtgg ggaacacgtc catcagcccc accggaacgt ctattttttc cccgcgcggg
     2221 aacgacccgt tgccgctgaa aatcaccggc acgcggacct ccgtctcctc cagaccgacc
     2281 ggatgcggtt cgccgtggtc tcccaggatc acccacatcg tcgactcttc gcacccgagg
     2341 gagcccacga ggtcccgcaa aggacccagt tcgttccggt cgaagaaatt gacggcgtcc
     2401 agatacgccg ccaacctttt gtccaaagcg cgaccgcagg gttcccgcaa cgcgcggtaa
     2461 agcgcgtccg gatcggcgat gggccggccg gcgcgccgct cccattgcgc ctcggtgaga
     2521 taatcgtgca cggaggcggt gtgaaagaat acgaaaaatc tttcccgtcc atgctcccgc
     2581 agccagcgga aaaccccgga aagccgttct tcgaaaagat gcgtgctgat gtcgcggagg
     2641 tcgtcaaaac ctcgacggaa accgaacgaa tccgaatgga agatgtgcgg catgccggag
     2701 gcgctgaggg tggagaaccc ggccgccttg aagagttccg ccaggagggg cacgccggcc
     2761 gggacctttt cctgtccgaa caacgaccgc acgccgtgtc gccagggcca ctggcccgtg
     2821 aaaaggccgg cgtgcgcgtt tgtggtgtat ggcgaactgc tcaccgcgcc ggaaaaatga
     2881 acccccgtcc gcgccagggc gtccaaaacg ggcgtccggc ggcgcgccgg caacgcgggg
     2941 tccacggcgg ccagataacg cgtgtcgtcc tcgcacgccg gggcgtcgaa acgcagggag
     3001 tcgacggaaa cgaggacgag gtttcgggga gcggcggtct ttttcatttt ataggatcga
     3061 tttcccacgc aaggtcctcg gaagggggtg tcccaaaagt ttcagcaccg tcggcgcgat
     3121 gtcgatgatc cgtatctttc gccggcccct caacgggacg tccgtcccgc ccagccaaaa
     3181 aaatccgttc cgggcatgga ccgcgtcatc cggcccggtg tcgttttccc gcaagtagag
     3241 accgttgtgc cccagggtcc cggcggaacg atggcccaga tcgtccaaat agaccagcag
     3301 gtccggcggc tggccccgcc acgatttatc ggacaagagg acctgattct tccagccctc
     3361 tccccccggg ccgcgcaagc cccggaaagc gcgggccagt ttccggcgga gcggctcgta
     3421 gtccctcctc gacaccgcgc cggccggttc ccgccccttc acgttgatga atatccgggc
     3481 gtaatacccg ccccagcccc aggcgcgggt gcgcggccag tccacgtcgc aggactcgat
     3541 cggcgtcggc ttttccggat atctcttcaa tttcaaataa ccctcccgca aaagccactc
     3601 gttcacgcag aaacatccgt tcatgcgttt cgacccgtga tcggacgcca gaagcaccgc
     3661 ggtgtccgcg gggagctccc cgaagagcga ttccagctgc cgatccaaaa atccgtaata
     3721 gtccgggatg gccctttcga attcgttccc cggccgatag cccggatgtc ccttgtcgaa
     3781 aaacttccaa aacgcgtggt gcatccggtc cggcccgatg tccacgaaca tggcgaaatc
     3841 ccacggatgg tttttcagga ggcgcttcgt catttcgaat ttatgccggg aggtctcata
     3901 gatctgggcg agtgtcgacg cccggccttc ccgccggaat tccgcgtcca gccggggccg
     3961 ggcgccgagc caggactcga tctgcttcat cacggccggc ggcgtggcgt acggcgcgga
     4021 cgaaggcgtc aaaagacagg agacccacgg cccgcggatg cggcgcggcg ggtaggcggg
     4081 cggaacgccc accacgacgg agggccggcc ccgtcggccc agataatccc aaaccgccgg
     4141 ctcttttatg gaggtcgacg aggcgagccg gatgtccgag tagggatttt cccccctctc
     4201 ccggaaaccg tacaccccca gttctttcgg ttctttcccg gtcgccagga cggcccaggc
     4261 gggcaccgtg atgggcgggt cacaagaaac catttcgccg tacacgcccc gcgacatgag
     4321 gcgccggacg aacggcatct taaaatcggg gtggtcgaag aacaattccg gggggatgga
     4381 atccaaaccc gcgatcagaa cgcgcgtcac gcgacgatgt ccttggccga tggggaagga
     4441 gggacggggg gagaaacgga gggggacttc aacgtcttat gaaaagaagg tcactttccc
     4501 ggcgtgacga ggttctcctt cttcattttt tttatgatgg cggacacgtc ttttttgacc
     4561 gtggcgactt tttggccgta atcctcggcg acctcctcgg cgatggcctc ctcggaaagc
     4621 cccttgccga tgagttccca aatcttgctc ccggtctcgt tcaacgaata atacacgctg
     4681 gtttccaaat ccaggatgac gacctcgtcg tccacttttc tccacgccac gtgttttgca
     4741 tggcgcccca ccgcttgccc gatacgcata gttcctcctc taaaaaccgc gactcaccgc
     4801 cacaagatcc cgctgtttcc gtaacttcgg tccggggcgg acacccgccg ataaaacgca
     4861 tccaaccgct ccgcgcattt ttcccaggac aggttcttct ccacccactg ccgcgccggc
     4921 aaaaccatcg actgacggag cgttttattt tttagcaaat taatcgtctc tttggcaaac
     4981 tccccggcgt cgcggcccag acggacgaag cccctcgtct ccggagggaa cccggacatg
     5041 gcggacgggg tggccgccac ggggagaccg acggcgaacg cctccacgat ctttccctta
     5101 atcccccacc ccagccgggt gggcacgacg aacacgtgcg ccgccttgaa cgcgccgacg
     5161 atgtcctcca cgtatccttc gacgcgcacg tttttcccgg cccgctcctt cagccaggcc
     5221 ggaacgccgg cgcccacgaa cgtgatgcgg gcgtcgggga gctcgtcgag gatgagcggc
     5281 catatctcgt ccaaaaaata ccgggcggcg tccgcgttgg gcggatgatg gaaataaccg
     5341 acgaaaagaa gttccggccg gcgcatctcc ggctcgactt ttcggaacga gaaccgcgac
     5401 aggtccaccc ccagaggcac atagccggtc ggcacggtcg agggccccca cgtatcccac
     5461 caacgcatgt cctccggact ggtcaaaaca accccctgaa acgcttccgc cgtccaataa
     5521 tggctccaca gtcctttcaa ccacgcccgc acccgttcat ggacgggcca accgcgccgg
     5581 cccgcatgca acgagttatt gaaccatacc cagcccacat cgtgctccac caagatcgtc
     5641 gggggaccga aaacgaagcg gcggcacccc gccatctgga tatgttcgat ctcgaccaaa
     5701 tcgtagcgtt cctcccgcag ggctttctgg aggacgcccc ccatcctctc aaaagcgggg
     5761 ccttccgaga ctttcttgtc caaaaaatcc gtccacagat tttccgagga ggccgggacc
     5821 aggtaggtct tttcaaagag catggccagc cgcagtttca tcgccgagag agcgacccgg
     5881 tcgttgaacg tcgcctcgct gacgaacgtc gccaaggaaa acgcgtattt ctccttcaac
     5941 aaaccggcga gtttccatat ccggatgttg gtgctctcaa aaagcgggaa cggcggatgg
     6001 ggcgccacga tcaacaccct tttccggctg tccttcttcg tcttcatgcg ccggctctca
     6061 cgcatcttcg aacgtctcca tcgtcacgga aacggttttc gcgttggcga ccgtatcgcg
     6121 gctcagccgc cacaggtaaa cccgacggca tgccaaaagc ctcatcatcg tccgcgtccg
     6181 cgaaaaagcg ttcttcaaca acccccccgt cgcccggacg tcccaccgca aaagatattc
     6241 gcgcccctgc ggaacgccga ccccgaccag cagggacgag atcaacgccg gcgccgcccg
     6301 ccaagcggcg acccgtcgca acaatttctc ccccggcgcg gcccaccccc tttgtcccac
     6361 aaaaatggcc gacgggacga acggcccccc gacggcgatc cgtcccttcc aataatcgat
     6421 gtccaccaac atcttcggcc catattcttt tcggcggaaa acccgctgca tcgattccgg
     6481 gatcccgtcg atgttccggt cgccgcaaat gccgaccctc agcgggaacg gccacaacct
     6541 cccctgttcg tccaccagcg gcgtgtcctc cgaaaccagc cgcacgggaa aactccggag
     6601 cgcttccagc gcgaaggtgg ttttcccgcc gcccatcggc aggagcatca gcgcacattt
     6661 gcctccccat tccagggcca ggccgtgcac ccggtgcaac ccgcgccggt ccagcatttc
     6721 ccccacgcgg ctcaacagca gcagatatcc caactcatgc agccgatgcg gttccgcgca
     6781 aaagacgcct ccctcttcct cggaaaaatc gtaatccacg acggcctccg ggtcgtggtg
     6841 gacgaaccgc cgggaatttt tcttttgcgg caccgccgac ggcgggaggg tcgcgctcag
     6901 gcccaatcga acatgcggca tggacttttc cggatccgcc cggaaccatt gaaaatccct
     6961 ccggaattcc tccagaatgg attcagcctc cgattcgatc gccgcgcgca ccccatagaa
     7021 atccaaaaaa agcttcatgt gtcccgtcct gccgacggcg aattcaaccc cggccggagg
     7081 aaaccgcaca ccaatcccgc gccgtacgtc atatgcgctc ccgaaatcaa caggccgacg
     7141 caccccgcgc ctttcacgcc aagacggtct tcccacagcg cccgggccat ttcccacccc
     7201 agaaacccga ggtatacccc caacgggagg aagatcgccc ccgggcggac ccacggaagc
     7261 gtcagaagat acaccaccat cagactcggc gcggcgcaga cggcccggaa actccccggc
     7321 acgcgccgcc acatgtgtcc ccggcccacg ccggaatgcc agacctgcct caaaaatccg
     7381 ccccacccgt tccggcgctc atgaaaaacc tccagcgccg gagaatacac caaccggacc
     7441 ccgtcgttct tcaagcgggt cagcaaaacg ttctcctcgt tatagtggaa gtcctcggga
     7501 aacgcggggc ggccggcctg aaagagggcc cgccggaaca tcaggttgca caggatcagg
     7561 tcaccgtcgt cggccgcgcg ccggcgaccc aagggacgat accgcgcccg catgcggccc
     7621 gtgcccagcc acgacgtcaa cacccgcccc acgcaccgct ggaacggcgt ggcgtctttc
     7681 ggcgtgctgt tcggtccgcc gatcaccccc gcggccggtt ccgcttccat ctcctcccgc
     7741 atccgaaaaa agatgtccgg cggcaccgtc acatcgtcgt cgatgaaata gacccattct
     7801 ccccgcgccg agagcgcgcc gagattgcgg gcgcggcctt tcgaggaacg ttccagttcc
     7861 acccagcgaa aatccggccg cgacgccgcg tgtttctccg cccagccccg cgtctccgcg
     7921 tcggcgccgt tgatgacgag aacaatctcc cccggcagcg gcggccgaca cgcctccacg
     7981 cttccgatgc atcgcaccag aggctccaac cgtttatggg tgatgacgac gacgctgagg
     8041 aggatggatt cgggattggt cattcgatat tcggcaccgg caccgtttcg acaatgaggg
     8101 aagtgtaaca aattttcaac cgacgcccgc ccctcccgaa aaacctaccg gccgcgcaga
     8161 tacttcagga gttctttttc ttttatcgcc ccttcacgcc ggagaaccca atgataggtg
     8221 gaggcgtcca ggatggtgga gggctccccc accgggcacc ggccggcgtc caaaacgacg
     8281 ccgacttttc cgctgcagaa cgtccgggcc tgcgttccgc tgcgagccgg tgtcccgccg
     8341 gaaatgttca cgctggtggt cgccaacggc tggccgaact cccgtaaaag ttccagggcc
     8401 acgcggtgct tcgggatgcg gacggcgatg gtgtccttcc cgccggcgac ccagcggccg
     8461 agcggggacg ttttgaaaat gaccgtgaga ggcccgggcc agtagcggtc cagaaggcgg
     8521 cgcagccggg cggggatcgg ttcgacgagc gcccgcgcct ggtcgagatg cgccaccaga
     8581 aacggcaggg gctttttata ggaccttccc ttcaggcggt agatccggcc gatggcttcc
     8641 gggcggaacg cgccggccgc cagaccgtag accgtgtccg tcgggaaaac gaccagcccg
     8701 ccggaccgaa gactctccac cgccgtttgc acggcggtcc gctcctccgt ccgcaaggcc
     8761 ccgcggcccg caaccttcat gaccttcgtt ttcaggggaa caatccggga cacttttttt
     8821 cttctgggag gggagactcc gcccggctca cgtggtgtag tcggcgttga tggaaatata
     8881 gtctttggaa taatcgcagg tcacgtaatg ggcccgccct cggccccggc gcagatccac
     8941 gcggatgacc accgtgtcct tcttcagtat cttatggagc gctttttcgg aaaaagccgc
     9001 ccgagctccc cggcggaaca ccaccacgtc gccgaaccgg atctccgccg tggaaggatc
     9061 gaaggggacc ccggcccggc cgagggccgc gatcacgcgg ccccagttcg ggtccgcccc
     9121 gtgaagcgcg gtcttcacca gaggagaggt ggcgatggtc gccgcggccc gggccgcgtc
     9181 ctcctcgcgg gcggcgccct ccacgaagat ccacgccgtg cgggtggcgc cctcgccgtc
     9241 cgcggcgatc tgcgccgaga ggctcaaaca gacacggtca agcgcctctt gaaatttcct
     9301 ccgcgccccc gccgaactcc cccccacgcc ggccgcgccg ttggccaaca ggaaaacgct
     9361 gtcgttggtg gaggtgtcgc cgtccaccgt cagccggttg aacgtcgccg aggccgcctc
     9421 ccgcagatcc ttccgcaggg ccgccgcggg gagcgcgatg tcggtcagga gaaaaaccag
     9481 catggtggcc acgtccggat ggatcatccc ggcccccttg gcgcatcccc acacggtcac
     9541 ggtttttccc cgcacagaaa aacgttccag cgccgccttc ggacgcgtgt cggtcgtcat
     9601 gatggcgcgc agggcggccc cgacggacga cgggcggccg gcccgaacga gatccacgag
     9661 tccgtccacg ccggccttca gcgggaccgg aggaagatat cgcccgatca cgccggtgga
     9721 agccaccaac acctgggccg gagccacgtt cagtccgcgg gccgccgtgc gcgccgagga
     9781 ggcggcgtcc gacaggccgc gggcgccggt acaggcgttg gcgcagccgg aattggcgat
     9841 caccgcccgg acggaccccg ccgtccgaag atgccgccgg ctgagcaaca ccggcgcggc
     9901 gaccacgcgg ttgcgcgtga aaaccccggc ggccgcggcg ggccgagcgg aatagaagag
     9961 cgccatgtcc tttttccccg gtttcgacga gatgccgcac cggacgccga aaacggaaaa
    10021 acccttgggc aacgcggagt tcttcagcgc cgccgggcgg gccttcgcca gggccaacaa
    10081 tcttttcaac ggcagatcac cggccactca gagagcctca ccttcgtcga agccgaacat
    10141 caggttcatg ttctgcaccg cctggccgga ggcccccttc accaggttgt ccagcgccga
    10201 caggaccacc acccggttgg tccgctcgtc cactttcaga ttgatgtcgc agtaattcgt
    10261 gtgggccacg ttcttcgtct gcggccattc ttccccggcc aggagccgga cgaatttttc
    10321 ccggccatag tcgttctcaa aaaccgcccg caccgcggcc gccgacgtct ttttcttcag
    10381 cgtggcgtag gccgccgcca gaatcccccg gttcatgggg accaagtggg gcacgaaagt
    10441 gatccgcgtg ggtcggcccg tcgtctcgga aagcgtctgt tccacctccg gggtgtgccg
    10501 gtgatgcgcc acggcgtagg cttgaaaatt ttccgtcacc tccgagaagt ggtacatcga
    10561 cgacagcttt ttccccgcgc cggaaacgcc ggacttggcg tccacgatca acgagtcctc
    10621 gtccaccaat ttgtgccgca acagcggaag cagcgccagc gtggcggcgg tggcgtaaca
    10681 gcccggattg gccaccagcg cggcggaccg aatccgttcc cgccacagct ccggcaaccc
    10741 gtacacggcc tttttgagga ggtccggcgc cggatgttcg acgccatacc acttttcgta
    10801 cgccttcacg tccttcagcc ggaagtccgc cgatatgtcg acggccttct tgccgtgccg
    10861 gaggaactcg gcgatggccc ttccccccac cccgtggggc agcgcgaaaa acgccacgtc
    10921 cgactctttg gccaaccggg cgatgtcggg cttttccagg acgaggtcgt atttcttccg
    10981 gagagacgga atcacctcgt cgatccgctt gccggcggac gattcggagg tgacgtgctt
    11041 caccttcaca tggcggtggt tcaaaaggat tcgtaaaagt tccgccccgg tgtacccggt
    11101 ggcccctacg acagaaacgt tgatcaagga aacctccccg ttatgtctct ttgagttgag
    11161 gcgacagcac cacgatgacc tcgcccttga cggtctcgcg gcggcccagt tccaaaacaa
    11221 cgtcgtccag ccggccccga atgaactctt cgtgcagctt cgtcatttcc cggccgatca
    11281 ccgccgggac gttgccgaac acgtcccggg ccgcggacag ggcggccttc aggcggaacg
    11341 tggactccat gaaaaccacc gtgcggtcgg cgccgacccg ctggaattcc ttcttcacgc
    11401 ggccggcgcg gcgaggcaga aaacccagaa acgaaaattc gtccgccggc aaccccgacg
    11461 ccgacagcgc cgccgtcacc gacgacgggc cggggatcgg gaccaccggg acggaagcgt
    11521 tcaccgcctc cgccaccagc cgccctccgg ggtccgagat gccgggcgtc ccggcgtcgg
    11581 tcaccagcgc cacgcgttcg ccgcgccgca ggcgctccaa aagccgcggc gtctcccggc
    11641 gatgaacgaa attgtcgtat cgcaccacgg tcttgcgcag gccgaaatgg gccaggagct
    11701 gccaggtccg gcgggtatcc tcgcaggcga ccacgtccgc ctcggaaagc gtcttcagcg
    11761 cccggggcga cacgtcctcc agattgccga tgggcgtggc cagcacgaaa agcggcactg
    11821 tcaaactcct caggccacgg ccgacgggtg gcgtttctcc tcggcctcca gggaccgcag
    11881 gaacgcgtcg acgatcttcg ggtcgaactg gcttcccgcg cctcgtttca attcctggat
    11941 ggcggcctct cgggaaagcg ctttccggta gggccggtcc gacgtcatcg cctcccaggc
    12001 gctcaccacg gacacgatcc gcgcgcccag aggaatctcc tcccccgcca atccgtcggg
    12061 gtagccctgc ccgttgtacc actcctgatg ataaaggacc atcggcgccg cgggcgccaa
    12121 aaacgacatg ggggagatga tctgcgagcc gatcttggga tgtttcttga cgagggcgta
    12181 ttcctcgctg gtcagcttgc cgggttttct cagaatggaa tccacgatgc cgagcttgcc
    12241 gatgccgcgc atcatcgccg cgtattccac gtactggatc atctgttccg gcaaatccaa
    12301 ctgctgggcg atggcccggg catgttcatg cgcccggctc ggcgccttct ggacgttcgc
    12361 ctccttggcc tccaaggcct gcaggagcgt ctgcatcaga tcgatgttga attttcggag
    12421 actgtcgtag agttcgaaat tttccagggt gatcgccgcc tggtccgcca gaattcccag
    12481 caggcggatt tcccgctcct cgaaaaatcg ggccgactcc gcgttgacgt tcaacacccc
    12541 gatcacccgg tccttcaccc gcaacggcac gctcaccatg gcctgcgttt ccagcccgga
    12601 ctgggtggac cggagaaacc gcttgtcgga gcggatgtcc tccacgtaaa tcgttttgcc
    12661 ggtctgcgcc acccggccgg cgatcccttc ccccacccgc aaccgggtgg cggcgtcctg
    12721 gacggacaac ccctgagacg ccgagatctc cagcgtttct ccatcgaggg tcaggagcat
    12781 caacgaaccg attttcgtct gcgccagccg acaggcgttc tgcacgatgg tgtcgcacag
    12841 ttcccgacga ttggccggcc ccgtgccgct catgccgagc cgatagagac tcgccaacat
    12901 ggccaccagg gtgtccagcc gcgccacgtg ctgctgcaga tactcctggg tgtcctccag
    12961 gcggcgaatg tatttattgc cctgttcccg ctccagccga ccggcgcggc gaaggccggc
    13021 cagcgccacg atcaggagcg ccagaaccgc aacgagaacc cacagcaaca cgtccgcctc
    13081 ctccatgtcc gatggtcttc aaattcggaa agggagggat tcgaaccctc ggtacgggct
    13141 tacacccgta caacggatta gcaatccgcc gccttaagcc agctcggcca cctttcccac
    13201 gggaatgctg gccatgcggc cagccgtggc acctgaaagc actgaggcgc cgcttttgcg
    13261 cctcaccgaa agcaaagctt tcgaggagag tgctcaaggt gcacggtatt cccaaataaa
    13321 accagcaggg gagtcgcccc tcgggggcgc tgccggcatc caaagcgctt cggcgccgta
    13381 tttgcaccga gcaggaaacg aagcttcctc ggagcgcgcg cgggtgccat ccacccccaa
    13441 atcaattcgt atcgaaaaat cctatcgttt atggaatcct ttgtccatgc gcgttttgag
    13501 cgactgccaa ttcactgcaa atcgatggga cggccgcccc cactccgcca atctcatccg
    13561 cgacctcttg caagtccgcc gtggagagat gtccgcatta tcgctcggag accacaacgc
    13621 tgctgtgtcc cagtatccgt agaaaatcga cgaaaaaatt gccccgaatg gcctccttcc
    13681 ccgattttcc ggcgcgcaaa ctcgcggccc cccatcccct tcgggccgct caaccgatcg
    13741 gccgcgatcc gctcccgata agatcgagcc cgtcataacg ctcttctgga attttctatc
    13801 aaaagatttc aaaaacccat ttaaaactcg taatggacca tccagttcca gcggttcgcg
    13861 aaatatcgtt ttgcctcggg ttggtctttg tagatcgcct ccaaatacgt gtattccagg
    13921 cccgtgctga gggatttcgt aagattgtaa cggacgttgc cgtacgtttt tcggttttcc
    13981 agattggtgt tcagggggat cgtcgcgcca tcgcgcagtt tgacgcgggc atgccccgcg
    14041 gcggcgctcc atcgggtttg gagatattta atctccgccc agccgccgtc ctctttttgt
    14101 ttgaacccgg acaggccgaa atagtcggcg atgttttcgc ccgtataagc ctccccgctg
    14161 aaaaccagag acccccattt gaccttcaag tccaacgcgc cggcatcgac ggaccctttg
    14221 gcgccggcgg cggggagggg atgggcggtt tggctttcgc gtccatacag accgctgacc
    14281 cccgcgtcca ggtgcgcgga ttggtaaccg atgcgcgctt ggccgtcggg ccgggccatg
    14341 ttttcacgcc ggctgtaggc gcgattgatg gacgcctgaa aacggaacgg gccgaaggac
    14401 gagttctcat accggacttg cggccgccag aaaccgatgt tgcccgaata caggcccagg
    14461 gaaaaatcga acgtgtcggg aagcagcggc gagacgacgt cggccgtttg gccggccaat
    14521 acgacatgcg gcccgcgctg ggcgctgacg aaggctttcc gcatgcggat gccgttgctg
    14581 atctcgttgc tggccacctg gctttcgtcc ccgtaaaaat ccatttccac cgcgccggac
    14641 agattgtatc cgtcctcttc cttggcggct ttcagtccga accgggtctg ccgcgcgtgg
    14701 atattgaagt tttcaccttt gctgcccgtg acatagaaaa cccggtcgcc gttgtcgcgg
    14761 aacatctgtt gagtgtccat atagacggtg tccacgtgca caaatccgta aagcgtcacg
    14821 tccaaagcac gcgccgcccc ggggacgatc agccccatta ttgccatcca tacgcatttt
    14881 cttctattgg ttcttcgtgt catataaaca tctcctttta taagccgaaa cagatctcaa
    14941 tccccatccc gcccttcccc tgaaacggga aggcttccgg acagaaatgt tccctcacgc
    15001 cacttcgctc aatttgggat ctctggtgcc gttgaggtat ccatgccact tgtaagacat
    15061 gtccgccaaa atcagtatgg cgaggaggac catggtgaca atcaggaact ggtcgatccg
    15121 gaggttcagc gcctccgcgc ccgtcgacgc ccgcgccgct tttgccgaga aaacgccgat
    15181 cagttgccaa gcggccgtga aggtcgtgac gtacatgaac gccatgggga tcaaggtcgt
    15241 ccaggcgtat ctcgccttgc cggacttgat gatgagggtc gtccccacgc cgaacgccac
    15301 ggcggccaga agctggttcg ccacgccgaa catgggccag atggtggaga tgcttcccgc
    15361 atggatgagg tagacccaca aaaaaacaat gatgaaactg gtgatccata tattcaaggc
    15421 cgtccggtgt gtcgcgatgg gcttgtaaat ggcgccgccg atttcctgaa ggatgaaccg
    15481 tcccacgcgg gtgcccgtgt ccaccaaggt caggatgaag agcgcctgga acatgatggc
    15541 gaagttatac caataaggca tcgacgcggc gccgcccaga atgccggaga ggatggcgga
    15601 catgcccacc gccaaagaca ccggcccgcc gggccggccc gccaattcga tgccgacctg
    15661 ttgcgaaaac tccgttattt ttccgacggg gaatcccagc gcggccagct ggtcgaaagg
    15721 aatcggggcg ttgatggcga aatagtcgcc ggggatcagg atgctggcgg cgatcagcgc
    15781 cgtcacggcc accaggctct cggtcagcat ggcgccgtag ccgatcaacg gaatgtccgc
    15841 ttcggacatg accatcttgg gcgtcgtccc ggaagaaatc aatccgtgaa accctgagat
    15901 cgcgccacag gcgatggtga tgaaaaggaa cggaaaaacc gttcccggaa tgatggggcc
    15961 gggctcggag gcataccgcg tcaccgaggg catctgcagg gtcggagaca tgatgaatat
    16021 ccccaccacg aggagaagga acgtgccgat ctttatatag gtggagatgt aatcgcgcgg
    16081 gcaaaggagc acccagaccg gcaaaaccga ggagaggaaa ctgagcgcgc cgatgaaaag
    16141 gatgagatgg ttccgcgaaa gagtgaagaa gggggccaac gacgaatcgg ggatgaaccg
    16201 cccggcataa accgccgcca acaacaggag cgcgccgatg atgcttcctt ccctaatctt
    16261 tcccggtctg aatttaaaaa gataaaaacc catggcgatg gcgatgggaa tggtcaggga
    16321 aaccgtgaag gtgccccacg cactgcccgc aagggcgttg atgaccgcca accccgagcc
    16381 ggaaacggcc acgatgatgt tgaacaggat gacaaaagcg gcggccatgc cgctgacaac
    16441 accgagttcc tctttgacga tctgcgccag ggagtggccg ttccgtcgga ccgaggccaa
    16501 caggatgatc atgtcgtgca cggcgccgcc caatgtggcg ccgatgagta tccacaggaa
    16561 tcccggaagg tagccgaatt gcgcggccaa ggtgggaccg atgatggggc cggctccgga
    16621 aatggctgcg aagtgatgcc cgaaaaggac ccagcggggc gtcggctggt aatccatccc
    16681 gtctttcaga cggaacgagg gcgggatgcg gttggaatcg agggcgagga ccttcgaggc
    16741 gatgaaagag caatagaacc tgtaggaaag ggcgtagata cacaaggccg cgaccaccag
    16801 ccacagagcg ttcacgtttt cctggggtcg ccaaacgccg accaccaccg ccagggccgc
    16861 ggcggcgacg agcgaaacga tcagccagcc gattttgagg agggccgacg tcttacccat
    16921 tgaatttcaa ggccagcatc gtgatatcgt cggactgggg agcatctttg acaaaagctt
    16981 ggacatcccc caacacattc cggaccacgg ccgccacgtc cgcccccttc cgttccgaga
    17041 tcgtctcctt caaacagtca tccgtgaact ggcggccgga cgggtccatg gcctcggtca
    17101 cgccgtccgt gtagaggaaa aaagaatcgc ccggattcag tttcaacttt tgatcgacga
    17161 atttggcttc tggaagaggg ccgagcacaa aagccctgtt gacgtcgatg aattcaaagg
    17221 gactttccga agccgcgtga agcggcgggt tgtgtcccgc gttggcgaac tccatctcgc
    17281 ccgtccgtat gtccaaaacg gcgcagaaga ccgtggcgaa catgcaggct tcgttgtccg
    17341 gagatagaat tttgttgacg gcggccaaaa ttgccgccgg ggaactgccc ttgagggcct
    17401 cggtcctcaa caacgttttg gcgatcacca tgaacaaggc ggccgggacc cccttgccgg
    17461 aaacgtcgcc gatggtcata tagagccgcc gatcgtcgat gagggtgaaa tcatagaagt
    17521 cgcctcccac ctccttggcg gggtacatca cggcgtacag atcgaattcc ttgcggtcgg
    17581 gaaacggcgg gaacgtccga ggcagcatgg acgtctgtat ttcatgggcc gccttcaatt
    17641 cgccttcgat ccggtccttg gcggccgcgg tttcctgctg tctcctgtaa ttcacgatga
    17701 tgaagccgta aacatacatg ccgaaagcgt tggcgaaaac catggggaac gcgatgtttt
    17761 ccaccaaaac catggcctgg tcgaagggac gcgccaggag aagcaccaac cccatatgga
    17821 aaaactcaaa cagggccata taaatcgatc cctggaaagg ggtcacgatc cgcctcttct
    17881 tatagaggta caccaggccg cccccgagac cggccagaac ggtggagacc gtgcagggaa
    17941 tgacggtgaa cccgcccatg aaatagcgat gcgtcgcccc gatcagtccc gctcccaagc
    18001 cgacgaaagg cccccccacc aacccggcga gggcgggccc caggtcccgg gtgttggcgt
    18061 aggcgccgaa aatattgaac ccggccaacg ttccatagat ggagaaaatc ccgaaaatca
    18121 ggctcaacaa tatcccattc cgaatggtta tcttttcctg atctatggaa taaagacggt
    18181 tgttgatcat aatgtagacc acaaccatca aaacacagat gaattccgtc agattgacgg
    18241 agatatcgat aatggtttta agcatcgcgt ttaccccaga acacggatcc cgctaaaggg
    18301 ggatgcggcc tccggttctc cctctctcga ttgttgagat cgctttttcc cgaaaatctc
    18361 tccgcttcag atttttacga cgcgaaaccc gatgtcaaaa aaataggtgt aggtgtagtt
    18421 cgtatcgtaa cggtaagcgc agaaggcgaa attgttgtcg tccttatacc aactaccccc
    18481 gcgcttctcc ttatagacgg cgtcggcggc gcccgtcggg tcggtctggt cctccggtgt
    18541 gtaagtgctg taccagtcgt tcacccattc ttcgacgttg ccggccatgt cgtacagacc
    18601 caaagcgctc ttgccgccct cgtagctgcc gacgtcggtg gtgtgtccga cggaaagtcc
    18661 gaagttcatg aattcaggct tatagtcgtt gccccacgga aaaatccgcc catcgttgtg
    18721 cgtggccgcg tattcccact ccgcctcggt ggggagccgg tagccgtgtt tgccgtaaac
    18781 ggcgcaggac caatcggtca aattgtacag cgcatccagt ccggcccgtt cgctgagcat
    18841 attgcagtaa aaggccgctc cgttccacgt gacataaaca aggggtcttt tctccttccc
    18901 cttttcgacg acgaaagcct tgccgtcaaa cgaaatctca catttgattc ccttgaagtt
    18961 ggcgttcaag tcgagcagtt cctgcgagtc cccctgggca ttctgaacgg tgacattgtt
    19021 ttgataatcg ccggatagat attttttttc cagggcgtag ttgagcacat cgcaaaactc
    19081 ggcgttggtc acctcgaatt tgcccatttc aaacgaacgg gtcagggtca ccttatgcac
    19141 gggaaaaccc aggcgcaagc cgttggggaa aatatacccc atggcgaaac ttcccgccgg
    19201 gaccttcacc atcttcggga aaagtccggc ttccttcggt ttcgctccga ggaaaccggt
    19261 ccctccgctc aacgccaacg cgacaaccaa acttatcttt aatagttttt tcataaatcc
    19321 cccctcaaat ctcgttcgtt tttggaaatt ttcaagtcca cggatttccg cgtccgcatc
    19381 tcaggctttc gccacactca cgccggccgg cggcgccgcc gccagcatct tgccggcgat
    19441 acggaacaaa accgggacga tgagccccca aacaaacgcc gccaacaaca ggctggtctt
    19501 cccgtaattg agcgtcccca cgcccatgac ggacgcgttg tgaaaaatgg ccacgccgaa
    19561 aaggaaaccc accacgaaac cgcggatgat cctgccctgc atgaaccaga gataatcccc
    19621 catcttcaga gcgaagttga tccacaaggc cagtatccac agagggcaca ggggggcggg
    19681 caaaaggaag cgggtggatt ccgcgggcgt gtacaacccg gcggcgatca tgatcgtttc
    19741 cgtcagaaaa ccgatccccc ccaccagcag cacgaccttc atttgctgac ggcggttttt
    19801 tctcccggcg aaatggatga ccgcgagcaa cgcgaccacc gcgggaccca gccaggggat
    19861 cccccgcacg gcgccctgat aacacgccaa gatcccgacg atgaacaccg ccgtcgtgac
    19921 cagttgatgg acaatgaacc ggaggaacag tttcctttgg gccgcttttt cgtcgttcat
    19981 ggcggatccc gtcttcaggg cttcgccggc tcgttgtagg gattgaacgg cggataaata
    20041 tccggttcga cgaccacgtc gatcaaggcc ggtttgccgg acgccaatgc cttctggaac
    20101 gccggggcga agtcctcgat cttctcgaca cgaaaaccca gcgcgccgca cgcctcggcg
    20161 tacgccttga aattcggatt caaaaagcga ttgaacactt cggccttccg gatccgcaat
    20221 tgaaacatct tgatgatgtt gaattcgttg ttgttgaaaa tgacccacac cacggggatc
    20281 ttgttctcca cggccgtcat cagctcgaac ccggccattt ggtagtcccc gtcgcccacg
    20341 gcggcgacga ccgtgcgttc cggcacggcg catttgatgc cgatggacga attcacgttg
    20401 accgccatgg ggccgaagct ccccgggttc tggtagttct gacccttcgt gagtttcaga
    20461 taagcggcca gccagagcat gtgcgcgccc gcatcaccca gaataatgct tttttccggc
    20521 aggtgcttgg atatttcctg aacgagttgg cccggatgga tcttgggccc cttgtgatcg
    20581 atcttttcat ccgcgaattt tttttcgacg ggcattgccg ctttttccac ccggctgacg
    20641 cgacgcccca ggctttcgtt aagtttttgt atcgccggtt tgacgtcgga gaccatgtaa
    20701 tagtccgcct gatagtagcg gctgatctcg tggggatcga tgttgatccg gaacaggagc
    20761 ttatcggcga aaaggtcctt attgaacatc caggtggacc acttggcgaa agaattgccg
    20821 atggcgatca cggcgtcggc ttccttgaag gcccgcttgg ccccgaggtc cccggaaatg
    20881 ccggtcatcc ccaaatgcag ggggtggtct tccggcagca tcccctttcc gtccatggtg
    20941 gtcataaacg ggatttggaa tttttccacg aacgccagga gttccttttc ggccccgctg
    21001 cgcacgcagc cgtagcccaa aagcgccacg accttcttct ttttttccag gagtccggcc
    21061 aacgcatcgg cgaactgttc gacgtttttg gccaggggca ggacgggcct gatctccagc
    21121 ttgatcgggc ggacgttggg cacctccacc ttcattatgt tgtaatccac gtgcaggttc
    21181 accgggccgg gccggccttc aaaagccagg ttgatggctt cttcgaggat gtcgcaggtc
    21241 tgttccggcc tttcgatcat gaaggccccc ttcgtggtcg cacggaacat ggcttgcgtg
    21301 tccggcgtgc ggtgtagccc cgtggtttcg cccagatctc ctttgccgcg atactccttc
    21361 ggggcgtacg gcgagatgca aaggatgggc aacgaatcag acagggcgac cgccactccc
    21421 gagaaaaggt tgatgacgcc gggcccggcc gtggagaaac acacgccgag cttgttggaa
    21481 aacatcgcat acccgcaagc catgaacgaa gcggcctgct cgttgcggat gatgacggtc
    21541 tttatttttt tggacttttc cagggcgaac aaaatgtccg aatcggactg tcccgagccg
    21601 ccaaaaatcg tgtcgactcc gatatcttcc agcgttttaa cgattacgtc tccgatattc
    21661 atggttgtat tcccatcctt tcgcttttcc gcaagtcttt ggcgaccttc ttcatccgaa
    21721 aaacactttt cgcgcggagg tcagcaccat tttatggcgc gaaccaccgc ggcgccagcc
    21781 gaatctcccg ttaatccgtt acttcaagac cagcggcttt ttccgggaaa ccgtccggcg
    21841 atgcgtgaac gccggccatc ccaaaacaac cgccgaatag acctgatggc cttcgggaag
    21901 attgagcatt ttccccaaac gcggcggatc atacaggcac attcccacga tacaactgcc
    21961 cagccccatg gcgtgggcct gcaacatgag atagtccatg gcgacgcaca cgttgtacca
    22021 ggaaaaaacg tctttttgag gagcgcaccc gaaaacgacg cagggcgctc cacggaaaat
    22081 ggggtgtcct cccttgtcga acgcgtccag gatagcccgg gagcgtttca tttccttgct
    22141 tgtttccggc atccccttgg ttttcatcag ggccacaaac gcccggaacc cgtccaggac
    22201 ggccgattct attttctgta tcgcctcctt gtcgtcaagg atcacgaact cccggtcctg
    22261 cgaatttccg tcggacggcg cctgggaagc cacccgcagc agccggtcca gcgcctgtct
    22321 ttcgaccggt tgggacagga aatgccgaac ggaacgtttc gaggacaaaa acttctgcat
    22381 ggtgtcgtcg tcgacggagg gctccacggg aacgaagtct tccgcgttca actccgcgtg
    22441 actcagcgcc ccggtcggac acaccgacac gcagtggccg caacgcagac atcccacgtc
    22501 ttccaaaaaa cgcggcggct tccccttgct ttcctggacg agggctgcgt agggacaatc
    22561 tctcacgcac aagccgcagc ctcggcattt ctgtccgtcg acttcaacca gtggcgtcat
    22621 gacggcccgt tcctttggac ttcctgtttt ttcgccgatt ggcacagcgt cccctgttgg
    22681 gccacgaact tccatcggcc gttttctttg acgtagacgc gcgtgtacag gtaagcgccc
    22741 ccgatggatt ctcccgcgcc cttggcttcg acggtatttt tcagcgtcag cacgacggtg
    22801 tcgttcccgt aagcgcgggc tttgacgtcg tcataacgga gcgtttcata cgccgcgttc
    22861 ttgacccact cgagataagc ttttttgctc gttaccatga acccgaacgg cgtgatgtat
    22921 gaccagtcct cggcgtaaag gtcgtcgaga tacgcgatgt ccttccgttg aagggcctcc
    22981 cccctttgac gatcgagttc gaggacctcc tgctccatgt gggcggccga cggagatcgg
    23041 ttctcgccgg tcaaaacgac ctcgcgctcc gtccgaagga cggacgttgc tctcttttcg
    23101 gttttcgctg cgccatggcc attacttggc caaaacctcc accaattttt tcccgtaggc
    23161 gatggacgac tccaccgaaa ggcccgtcac gaacgggtgg tcatacgcca cgcacggctt
    23221 ttcccgggtg gccatatcgt cggagatgca tttgcctttg gggccgacgg cgtcttccac
    23281 gatgtcctgc agggggatcg cgaaacccgg cgtcatcaag tccgttccct tgttgtcggg
    23341 ggacgcgccg aggagatcgt aggtcaggtc gaaatccacg aaatcccatt tgcgcggatg
    23401 cgcggtgacg gtcttgccgt agatgacgct tttgtagccg ttgttcggat cccgcgtgaa
    23461 cgcgagcgcg ccgacggaat aacagatcgc ccccaccacc tttttatttt tcacacagtg
    23521 aaggatcatc cgatgtacgg ccgggttgtt cgtgatatcg agagggtttc ccggcccgcc
    23581 ggtcatcacc aaggcatcgt aatcctcgat tttggcgtcc ttgattttta tggggttgtc
    23641 ccactcgccg ttggcgagga gttccttggt ccgtttcacg acttccggcg tgttgacctt
    23701 caccttctga tagccgtcca cgaactcggg atccatgctg atcttgatgg ggagcggttt
    23761 ttttcccaaa taggtggcca acgtcacctg atgtcccgct tttttcaaag catcccaagg
    23821 cgcctgcaat tcttccgccc agaccgagta atttgtcgcc aatacgagaa cttttttgcc
    23881 catgtcatat ctccttttct attcaattta aaacgattta caactcttcc cgatcgccgc
    23941 gctaaacctt gtcggtataa cctttcactt cccgcaacgg actgaataca tcgatggcct
    24001 cagtttctcc gacggaagtg aacgaatggg gttcgttcgc cggcacaaaa taagcgtcac
    24061 cggccttgag gatttccatc cgtgccccga ttttcacctt aaaaccgccc ttgatgatgt
    24121 aaccgaactg ttcggactca tggctgtgtt ccggcaagga cacgccgtcc acgaagttcc
    24181 agtgcatcac gttcatccgg cggccacccc ccagatactg tccccaaaca cccttgaaag
    24241 cctccttctt ttcaatgctt tcgtgatgtc gatatatatc gtccgccatg attcctcctt
    24301 taattgccaa aaccgcccct aaccacgggg ggcaatcaaa tcaatgattt tccgggcaaa
    24361 gggcgcgaag agcccccccg tgcgcgccgt gacgaggtcg tcgtcccgaa cgatgtcctg
    24421 gtccacatat tgggcgcccg tgttgcggac actgccaatg acgttgttgt ggcaggtgac
    24481 cttgcgcccg cgtatcactt ccggaatggg atcgaagatc cacaacgcat ggcagatcag
    24541 tcccttcaga atctttttct ccgccatgac ccgtttcatg aactgaacgg cgggagccag
    24601 atcgcccggt ttttccgcgt accgcaacat gtccgccacc atccccgcgg gcacaatgat
    24661 cgccgcatac gagcggagcg ttgcgtcgtc catgttttca aagcttttct cgaccgtcat
    24721 cgacatccca agttccagcc ctttgaacgt cagccggggc tggccccaaa gacgcgtcaa
    24781 gaattcgacg tccatccctt cttccttgaa ccggttcgaa taatagacga tctcctggtc
    24841 aatgaaccga ttctccaaca gaatccctat ttttttcaca gaagccatat cgtctcctcg
    24901 gctatttgtt cttgtatagt gtcaaaacgt tgcgggcacc ctcgcgacga taaaggacct
    24961 tgtccatgat ttttcgaatc atgaagatgc ccagcccgcc gatctttcgc tgttccatgg
    25021 acaggttggt atcgggcgcc ggcaacgaaa gcgggtcgaa cggaattccc gaatcggaaa
    25081 tctcgatgca tatcccgcga ccgtcctcga cggggccgca cgccacatcc acgctccccg
    25141 gaccggacgg atacgcgtaa cggaccacgt tgacgagagc ctcttcggac gccaaccgga
    25201 gttcgcccat ttgttctttc gaaaacccca cttttccggc atgttcgaga acgaacgcga
    25261 tcatgccttc cagattatcc aaaacggccg gaaaggtttt cctttccatg gggggtcctc
    25321 ttcttatccg gatcaaccgg ggtattgatc ctggacgatc atgaagttgt ccaagccgct
    25381 cattttaatg acgttcaaca ccgccgccgg ggctccgatc agatagagtt tggcgtcggc
    25441 gccgagtttt tgcttggcga aaataatgac gcgcaagccc gcgctcgaaa tatattcgag
    25501 atccttggcg aaaaaaacca ggttggaaat tttctgcgcg ctcaactccc gcaatttttc
    25561 ctgaaacttc ggggcgctgg cggcatcgag acgtccggtc aaaacgattt tcgccgttcc
    25621 atccttgcat tccatcgaat agcttgattc cataagctac ctcctttaat taggttcggg
    25681 gtttccccgt caataggacg atggagcggg gccccaccag aaattcacct tgattctcgg
    25741 cggccttttc ctgtccgact tcccaaatat cgtggggcgg ccgcatgtcc gtgttggcaa
    25801 acacgtgcca agccaacccc ccgggcaaaa cggggagctc aaagccgtgc atgtcccaat
    25861 gcatgttcat ggccacatag aggaacatat cgttcggccg gccggccaag gcccccgact
    25921 cgcagagcat catcgccaaa caccggctct cggcagacca gtcgcagttc caggctttct
    25981 gtccgtgcca ggacaggcat gggtaaccgc tggaattccc gcggttcaaa agatcgatgc
    26041 agcggagggc cggatgttcc tttcgaaaag cgatcatctt ccggaaaaac tgaaaaagtt
    26101 cgtcgttctc cttcaacagg cgccagtcga tccaagaaag ttcgttgtcc tggcagtagg
    26161 cgttgttgtt gcccccctgc gtgttgccca tttcgtcgcc ggccaaaatc atgggcgtgc
    26221 cgtggctggc caaaaggagg gccgcggcgt tcttgatctg ttttgcgcgc aaacgccgga
    26281 ccccttcatc cgtcgacggc ccttcccatc cgcagttcca actgaggttg tcgctgccgc
    26341 cgtccgaatt gttctcgccg ttggcttcgt tgtgtttgcc gttataggac accaaatcca
    26401 tcaacgtgaa cccgtcgtgc gcggtgatga aattgatcga agcgcgagag ccccggtttt
    26461 ccgactggta aaggtcggga gacccctgaa gcctctgtgc catgccgccc aacgcgcctt
    26521 cgtccccctt taaaaatttg cgcacgtcgt cacggaattt tccgttccat tcggcccaac
    26581 gcccccacga cgggaacgac cccacctggt acaggccgcc cgcgtcccac gcttcggcga
    26641 tcagcttgca tttgcccaga atgggatcga acgccagggt ttccaacagg ggcgggctgt
    26701 gcatgggcgc gccgttctga tcgcgcccga ggatggaggc caaatcgaac cggaacccgt
    26761 cgacgtggta atccgccgcc cagtagcgca ggcaatccaa aatcatgttg cggacgatgg
    26821 ggttgttgca gttcaaggtg ttcccgcagc cgctgaaatt gtagtaatac ccctcgggcg
    26881 tgaggatgta ataggtttta ttgtcgatgc cgcgatagga gatgtacggc ccccgctcgt
    26941 tcccctccgc cgtgtggttg aacacgacgt ccagaatcac ttcgatgccg tttttgtgga
    27001 gttccttgat gagcgccttc acctcgtcca cctgcatgcc gaattttccg gtggcggcaa
    27061 aacccgcttt gggggcgaaa aagcccacgt tgctgtagcc ccagtaattc atcagccgtt
    27121 cgccgttcac cggagaaatc cgggagttct caaactcatc gaattcatgg atgggcatca
    27181 gttccacgca attaacgccg agatttttca gatatgggat tttttcgcgg atggaagcga
    27241 acgtgcccgg atgtttgaca cccgaagacg gatggcgcgt gaatcccctc acgtgcattt
    27301 catagatgat caaatcttcg atggggtttt cgagagggtg gtcgccctcc cagtcgaaat
    27361 cgtcgtagac gatgcgggct cggtgaggat acacgttgtt ccagtcgggg atttttcccc
    27421 agacgtcccg gccgccgatg gctttggcgt aggggtccat caaaatcttg gttttgtcga
    27481 accggtgacc ttcctggggg cggaacggcc cgtccatgcg gaacccgtat tccagttcct
    27541 cataatccag gtcgaaaacg accatggtga acacgttgcc gatccggaac tcgtcgggga
    27601 aaggaatgac cgcgtagggc tggggttcat gcttgcggaa caaaaccagt tcgcaggaag
    27661 cggcgtgcct ggaaaaaacg gagaaattga cgcccccggg aacgatggtc gccccgaacg
    27721 gcagaggctt ccccacgcgc aaatcaaatt ttgcgtgttt atgcgtcggg tgggagtcga
    27781 ttcgatgcat gcgcctatcc ggccttcaaa gcgcggacag cttcgtcggc ggaggggtgg
    27841 tttttgaaaa accccccgaa accggtcatt tccatcacgt ctttgacctc atcgccgacg
    27901 ccggccaaag cgaccttgcc gcccctggaa gcggtctgct tggccaccaa gaggagaact
    27961 ctcaatccgg cgctggaaac ataatcacaa cgagacatgt ccattacgag acgggcgccg
    28021 ggaagaacga ggggcatgat cttctcctgg gcctgcgcgg acgtgttgcc gtccagcttc
    28081 ccttcgacag tgatgaccga tacgccgtcc cgcgtttcta ttttcagttc catgtccggc
    28141 gccttatttt ccggtcggaa ccatacggac tttgatcttg gggcgatagt tcagcgtggg
    28201 caattccacc gtgagttttt ccgcgtcgaa ttttttccag ggattgccgt cgatccagac
    28261 gtcggccaag cggacgcttc ccggaggaag gatgtccggc tgaacgcgga gcacgctgtc
    28321 ggcgaagccg ttgacgacgg gtttgaagta gagttccagt ggctgcccgg tgctgagcag
    28381 gttggtgtag acctgcgaca ggtagcacag ttcgatggaa tggtacccgc tcatggaatg
    28441 gcaccctttc aagcgctcgt tccccatgag gtaggggatg ccgtggttga ggacgttgaa
    28501 atacacggag ccgtcgtcgt ggtccaggaa gaacgtgttg tagaaagccg ccgattccct
    28561 cgccagcttg agatactcgg gatttttcaa cacgccgaac atgatcatgt aggccaaaat
    28621 gccctgctcc tgctgccacc aggctttgcg gtcgtgccag caaaagcgat ggacctcttc
    28681 atccccccgc agttcgcgtt caaccacgtc gtaccaaccg ccgcgctgaa cgtccatgcc
    28741 gaccttgggc atgagctcgg cgatcttctt cgagagttcc acatatttct tgtccggctt
    28801 ggcgctgttg atgcgcatga ggttccacgc gatcttgagg ttatgtccaa tcacggcctg
    28861 gtcctgctgc cagccccagg tcaggtcgcg gctccagtct tccatgaaac gctcgttcac
    28921 gaaggggctg tgtccgtagt cggggaaatg gttgacgatg cagtcggcgg tgtaggcgag
    28981 gaagtccttg taccggttgt cgcccgtggc cagatagagg ttgacgaggt aggcgggcgc
    29041 gtggtcgccc accgagttcc agttcttgcg cgccttattg cggcccagac tctcgctgcg
    29101 ggggtcgagc gtgatgggat cgatatggga gaaatacccg tcccgctgct tgtcgaggaa
    29161 aaaccggtcg aagaggtcga ccgtcatttt gatgtctttg aggatcaatg gatcgcccgt
    29221 cacgcggtag gtctgggtcg ggccggccaa cgcgtagatc tgttcgtaca tggggatagc
    29281 gtcgtaatcg tcgccgaact ccgaggcgaa tatcttgtgc tccccgtccc ccttgacgtc
    29341 gataccgtga taccagtaga ccacgttttc gtccgtgtcg tagaagcgca tgtggtcgcg
    29401 gagataggcc gtcccctttt cagcggcctc aaggaaacga tcctcgccgg tcaaaaggaa
    29461 cgtggaagca aaaccgtaga cgaggcgtga aatcgtgtcc gtttcctgac gatggtcgcc
    29521 cttgcgcgcg ccgctgagag acaacatggt gcggtagttg cggtaatcga tttccttgcc
    29581 caggaactgg gcccggatga aaaaatcggc gatgctggtg gcctgtttga tccaccaatc
    29641 gggctcttca aaacggtatt cgtcgacgct tttccccgga aaatcgatcg ttttggcttc
    29701 gaaaacatgc gtcccttttt ggggataaaa aacgccgtac gcgtacagat atcggccggg
    29761 aaccaaatgg tcgtggagct ggcccgtgaa atccacgtac gcttcgtcca ggttgcgggg
    29821 accgcgggca tacgtgctgg gcgtcagctt gacggagaac tcacgtccat catacgtttt
    29881 aagaccgaaa acccctttgg ctttatcgaa agcggtgaca acaccggcga tcgcgtcgga
    29941 aaactcgaag ttcatgcgtt ccatacgata gtcatccccc tattttttca catcgcacat
    30001 agaaatattg aagcctaagg accgcgtccc cgaaaaaatc ggcggtcctc cttttaacag
    30061 cgccccataa acgggggcaa ctccgttcaa aataatcccg ttgccgaaaa atcgaattac
    30121 gttttgtatc gcgctcccag cgcctcggtc aggaggggct ttccatggtt tcgcccaagg
    30181 cttgaagggc ctcttgtgtg agcgaagcct ctttttcgga ttcaaagcgg accacgaggc
    30241 tgtaggcgca cggaacgacg aagagggtca aaagcgtgga aagaaacacg cccccgatga
    30301 ccgccacgcc catgggcgcg cgcagttcgg cgcccggccc caacgtaagc gcgctgggaa
    30361 cggcgccggc gatgatggcc agggacgtca tcaaaatggg ccggagccgg agcgggcagg
    30421 cttccaagag cgcgtccctc acccctttgc cggcgagacg ccgcacgttc gtgaagtcca
    30481 ccagtaaaat ggagtttttc ttcacgatgc ccatcagcaa cagtatgccg atcatgctca
    30541 tcatgttgag cgagtggttc gacagccaga gggcgcccag ggcgcccgag atgctgaacg
    30601 gcagggccag caggatcgtg aacgggtgga tgaaactgtt gaactgcgcc ccgaggatca
    30661 tgtacgcgat ggcgatgccc agcaacaggg cgaaaacgag gtcctgaaag gcctctttga
    30721 actgctcgga actgcccgcc ggaaccatgc gatagccttc cggcaaaact tccttggcga
    30781 tcttctgcgc cgcggtcatc gccatcgcct gtgacttgcc gggcgcgacg ttgccgaaca
    30841 ccgcgacggc gcgctcgcga ttttcgcgcg agatggcgag cagcgtgggt ttttccaaaa
    30901 ccttggtgac ggccgacagc gggatcaatt cgccccgcac gttgcgcacg aaaaggcggg
    30961 agatatcgtt ggtcttggtg cggaactcgt ccgtcaggct gacacggacg tcgtaacgcc
    31021 gcccgccctt ggtgtatttt ccgacgcgca ccccgccgac gctggaattg atggtgcgcg
    31081 cgatggcggt caccgtgacg ccgcgttccg ccgccgcttg acgatccggc acgacgcgca
    31141 cttccggaac ccccacctga taactggtat ccacgtccat cgccaggccg cttttgagca
    31201 ggcggtcgcg gatcgcttcg gcgtattcgc cgagtttgtc ccactcgggc ccgatcacgg
    31261 aaaactccag cgggaaaccg cggccgccgc ccatggcctg cgaaaggtcc tgaatggcca
    31321 cccggcgcat gccgggaatg gcgccggcct ctttacgcac gacctgcatg aactcctgtt
    31381 gggtcaaggg gcggcctttt tgcggatcga tcggccgttt ttcacggtct ttcatggtca
    31441 aatacacgag agcggtgttg acctgcccgc cgctgccgcc gccgaccgat ccgaggaacc
    31501 gctccacttc cggccgggac aacaggaact tttccatctc cttgtacacg ttatcggtga
    31561 aagcgaggga agacccgggg agcgtctgca aacgacagat gaaaacgccc tggtcctggg
    31621 acggcagcat ctcgaagcga agtttcccgg caaggcccaa agacacgagg aaaacaaacg
    31681 ccgcccccag aatcaccttc caacgatgat tgagggaaaa cgtcagcaag cggtgatagc
    31741 cgttggtgag ccgggccatg aaaacgtcca tttttcttcc catccatgtg tcgtgcccca
    31801 cctccaagaa ccggctgcag cgcatgggcg cgatcgtaat ggcctccaac aacgaaaaag
    31861 ccaccgcgac cgatatggtg acgccgaact ggtagaagta ttttccgatg atgcctttca
    31921 tgaacgccac gggcagaaat atggcgatga cggcgctgga gatgacgatg acggaaaacg
    31981 tgatctcccg cgcgccgtag atggcggccc gaacccgggt ctccccctcc tcgcggtggc
    32041 gcacgatgtt ttccagcacc acgatggcgt cgtccaccac gatgccgatc accagagaaa
    32101 tggccatgag cgtgatggtg ttcagcgtga agcccagaaa ataaatgacc atgacggtcc
    32161 cgcccagggc cgtggggatg gccagaaaaa cgttgagggt ggacgtccag gcgcccagga
    32221 acagccaaca caccaaagac gtcaaaagaa cggccagcac cagcgtgacg atgagctcgt
    32281 gaatgttttc cttgatgaac gtggtggaat cgaaattgac ggaaatatct atgcccggcg
    32341 gcagatcttt gcggatatcg gcgatcttct tcttgacgcg gtcggccacc tccaccgcgt
    32401 tggcgcccct ttgcttgcga atgcccaagc ccacggcggt cttgccgtcg gccctcgcca
    32461 aacgccggac gtccgccagt ccttccttca cctcggcgat gtcttgaagg cggatcatct
    32521 tccaaaggaa cccctgcccc gcgcgcgtcg ggatcgagat ccggctgaac tcctccgccg
    32581 tggcggcttc gcccatcacg cgcacgttgc gttccgcctg cgacgtctgc aagacgcccg
    32641 ccggcagttc ggaatgttgc gcctggatgg cgctgacggc gtcgtccacc gtgagttgca
    32701 ggttgtgcat cttcgacggg tcgagccaca cgcgcaagtt gggttcgatg aacccctgga
    32761 gggacacttc ccccacgccg gggatcgtct ggaaccggtc cttcaggtgg tcctggacgt
    32821 actgtgaaat ctccaaatag gaacgcgtgc cggaaacgcc gacgaacagg atcggctggt
    32881 cctccggatt caccttttgg atgctcgggg gatccatgtc ccggggcatg ttgtactggg
    32941 cggcggcgag tttggtctgg acttcctgca ccgccacgtc gatgtttttg ctgaggtcga
    33001 actcggcggt cacgttggcg aaaccgtagc gcgacgtgga cgagatcttc cggatgcctt
    33061 ccacggacat gatggcttcc tccacctgat cgacgatctc gttctccatc acttccggcg
    33121 cggccccttc ccagctcagc gaaatattca cgttgggcaa atcgatgtcc gggttctgcg
    33181 aaacgcccat ccgctggaac gcgatgccgc cgaacaacag caatgaaatc atgagcatcc
    33241 aggcgaaaac gggcttcttg atggacagat cggacaaaat catttcaacg cctcctcggg
    33301 gcgagccccg gccgcgattt ccagtttgat ggcgttcagc cgggactgaa cttgaaagtt
    33361 ggtgagggac aattccgcca agttgaggcg gttcagcgat tcgagcactt ccaggctcgt
    33421 caccaacccc tggcggtcgt cccgcagttg gatcccatag tcgcggcggg ccagatcgac
    33481 ggccttggcc tgcaactcac attgtttttg cgccgacaag acatcgcgat aggcgttgcg
    33541 cacttccaaa tccgcctggc ggagaacgaa ccgaagcgtt tgctcctgga cacgaaaagc
    33601 ggccagggcc gaatccacgg cgccggttct ggcgccccag gcccagaggg gaacgttgac
    33661 gcccaacgag gcgtcccatt tcgtttcctt gtagaacttc ggccggtagg aataaaggtt
    33721 cagccccaaa tcgattttgg ggagatagtc cccccgcctg aaatccacga cccccttggc
    33781 cgcgctcacg gcggcctcgg ccgattgaac gtccggacgg gaatggaggc gcgacaaata
    33841 actttccaac gaccgcacaa aggtctgcgt ggaaacctcc gttgccggcg gcggctgcac
    33901 gggccggccc gacaggtagg tcaggaaatc cacgtaagcg cccaccgacc ggctatgctc
    33961 ctctccctgg gactggagcg cggtgatctg ataatcctgc tggatgggct ccgcgtttct
    34021 ggaacgcccg acgcggacgc gttcgcgcaa ttcgttaagc cggtcgttgg ccaacgcgat
    34081 cagccgttcc gtcgaagcga cgttttgccg ggcttccaca agaccataaa atgccccggc
    34141 cacgtcgacc aggagcagtt gttcgacgcg ctgcttgtcg tatttcgctt gggcaatgga
    34201 atggcggccg gcgcgcaggg cggccagttc gcggtatccg gtcagtccat atttttggat
    34261 ttgaaacgcc ccggtgtgtt gtgtgtcctt ccggcgctgc cagaacgtgt cgtgctggaa
    34321 cgataaatcc ggcaggttcg cgccccgcgt ctgtcggtaa agcgcttcgg cctgagaaac
    34381 ggactgttcg ctgatggcga ttttttcgct gttggcgagc gccgaaacat agacgtcccg
    34441 gagcgttgtc aagggaacgg cgtccgtgcc ggattcctgg gccatcgtca gggttggaag
    34501 aaccgccatg agaccaacaa taacgagatt caaaaatgtc cgcgttgtca taaaaacctt
    34561 tcgatgattt tttacgggcg ggcgctgttc aaaatcctaa agcaggtagt cccaaccccg
    34621 gaatgttggc gcgcaccgcg cccattcgat acgcgcttct gccggctcga acccatcgat
    34681 gaacaccccg gccccggcct tgtttagacg catgtcattc ataaatgccg agacctcagg
    34741 acgcaccgtc cccgaaagat ccaagttttc attataagcc gatgaaaaga cagagctacc
    34801 gacttcgaag aacaaccctt atatcacagg cttgatgttg cggaaatatc gcggtaaaaa
    34861 agcgacgggt tactttatct tttccccttt ccgatatcaa caagaatttg aacggcggag
    34921 cgcccggccg gggccgaggg gagggtgtaa aatcgcaggc gattttgcca cccatcgcct
    34981 tttccgccgg ccggccaggt ttgatataat ccgaacccat gcacccctct ctcccgcaaa
    35041 cgcctccgcc ggcgacgaag ccctggacgt cgcacctgct cccgctcctt gtccccctcc
    35101 tcgccttttc cctgttcggt tggtggcgca tcgacctgcc ggggcttcac ggcgaagaaa
    35161 tcggcccgat gggacccctt ctgggatgga tggtccacgg ccctttcaga ttcctcttcg
    35221 cgggatatca cgggccggtc gaaggccttt gttccatgcc cttcgttttc gttctggggt
    35281 cttccgtcgg ggccgtgcgc ctgatgcccc ttctcgccgc ccacggaacc atcgccgccc
    35341 tctacggctt ggccttccgc ctctcgcgga accgcgggac ggccttcgcg gccgccctcc
    35401 tgttcgccct ggccccgacg ttcgttgcgg ggacccgggt cggcctccag tccggggcga
    35461 tcctgctctt cttcacgctg ggcgcatttt tcttcggcca cgcctggctg acctccggac
    35521 gatggcggga cctcctgttg acggccggct ttctggcgct cggcgccgga tgccgcgtct
    35581 gggcggtgtt ctttgatgcc tctcttttcc ttgcgcttct cttgaccctc ccccgctccc
    35641 tgtttttctt ccgaaaaaac gcccgcccgc tggccgcggc cgggatcctg ttcgccgccg
    35701 tggcgggaac gccttacctt atagaatacg cgcgcggagc ggacgtgctg gcggccgtga
    35761 cacaacgaca ggccgagcat ccctccggct ttcaaaaaaa cgccgccgcc tgctttcgga
    35821 acatccatcg cttgatcgac ggcacgggtt atttttacga catggcttcc ccgcccatga
    35881 aagacgctct gaaccgtcag aaaatacgcc tggcgggacc tgcgattttt tggagcacac
    35941 tcctgtttgt ggtcttgaaa tcattattcc atcgcccctc cgtccccccg gttcttcaat
    36001 tccttgccgc cctgatcgtc tttcactttt ccggaaattt ccttgtaagc cacccccgga
    36061 ccgactacct cttcgtcctc ttgccgttca tgttcctctt tctcgctgtt ttttggggac
    36121 gctcctttcc tgcccgggga aagcgccggc atctccctct tctcctgttg tcttttttct
    36181 tcgtttcccg ttccctgtcg gtccttcgga cctacacgac ccatcttgcc gaaaccggcg
    36241 ggtgggccga atactccaca acattgaccg ggctgggaga aaccgtccga tgcctgggtt
    36301 ttcggcaggt tttccttccg gagggggaac ggccggcggg gaacaccctt cgttttctct
    36361 ccgcgcccca tggtcaagtc caatacattc ccgccgccgc ggcggtatcc gaacgtttcc
    36421 ttcgggccgg gccgaccacc gccttcgtcg gcgttcactc gtggcgaagc cgccggcccg
    36481 ggtccatcgc ctctctcatc gagaaaacgg ccgagcaaca gggttacgaa aagatgtcgt
    36541 tcttctcctt cgacgacgag cccctcgcgg acctttttta taaacccatc cccgcccgca
    36601 aaaaaccttc ccgttaaccg gccgcgccct ccgttccccc gcgacatgac ggatgtcatg
    36661 tccaaacacc gtcgagcatc atggcgcttc caacgccttt ccgctttaat catgtcttgc
    36721 gccacacggc ccgatgggcg gaccctccca tttgttatac tttgggaatg cgctccgatt
    36781 attacccgaa actcctgtcc gcccgccgaa cgttgagcgc gacggagaaa tcacggctcc
    36841 tcgaaacgtt cggtctccgc gtgcccatgt tccaccgcct cccgaaacaa ctggccgcgg
    36901 agctggcgga ggcctccggc gtgcgggcct acgccgccgg cgaattcctg tgccgcccgg
    36961 gagacccggc gcgcgagttg tgggttcttt taaaagggcg gatcagcatc caccgcacca
    37021 gctggaacgg gcaccgcgtg gggatcgaga tcctgctgcc gggcgacgtt ttcgggttgt
    37081 cggccatgct cttcggcacg tacccgaacg aaatcctcgc cgcccgggaa tccgtggtcg
    37141 cttcgattcc gaaaaccgtg atgatgaaag ccatcgaacg gtgcccggaa ctggcacggg
    37201 aaatcctcct cgtgttcgga cagagactgc ggttcatgga aacccaactg gttctgtccc
    37261 gggagagcgc cgaaaaacgg atcgttgcgg cgcttctcta tctctgccat aagttcggcc
    37321 cccggctgcc gctgacccgg gtcgaattga gcgagatgtc cgggacctcc ccggaaacga
    37381 cgatccgcgt tcttcagcgg ttgactcgcc ggggaggcct ccgcaaggcg cccggaacgc
    37441 tcctgtgcca cgtgcggacg ctcaagacga tgctggaagc cgaggaaaac cccggtccgt
    37501 cttgggacgt gcccgacggc ccgccctcct ccgtccctcg ttaacgtttt ttctgcgaca
    37561 catttttatc catcccggag gtccacgtat gcggaacacg tgtttttttg tattcggcgg
    37621 gatcgtcctc ctcgccggcc tggtgggtcg ggcagcggct cgcgcggagg agtcctcggc
    37681 ggcgtccctc gaagaacgct accgccaact ggaacaaaaa cagaaaatcc tggaacagcg
    37741 cctggacaac gccgacgccc gcgccaaaga aacgatcctc gtcacggccg accccaaaaa
    37801 cggtttctcc ctgaaatccc ccaacggcgc cttcgccctg aagttcaccg ggtacgccca
    37861 ggtggattat cgggcctatg tggacgacga cgccaaggcg ttcggcgacc agttcttgct
    37921 gcgcaaggtg cggccgacgc tcgacggcgt actgcacaaa tccgtggatt tccgcatcca
    37981 gccggacttc ggcggcggga cgaccgccgt tcaagacgcc tacgtggacc tgcattattc
    38041 acccctcgcc cgggtgaaag cgggtaaatt caagttcccg ctcgtcctgg aagccctgca
    38101 aaacgacacc gcgggtcact tcatcgaacg cgcctatccc agcgccctga cgccctcccg
    38161 ggacgtgggc gtccaatgga gcggcgactt caccaagacc gccctgacct acgccgtcgg
    38221 cgtgttcgac ggcggggcgg acggcaccag ccccaatggc gacggtgccg acaacaagga
    38281 tttcgtgggc cgcgtgttcc tccatccttt caagaacagt tatggatcgc tcctaaaaca
    38341 actgggggtg ggcgccgcgg gcagtttcgg caaggagaaa gggaccgccg ccgcccccgg
    38401 cgtggcctct tataaggaca cggcccaaca gacgtttttc tcctatcggt cggacgtggt
    38461 ggcggacgga aaacgcgtgc gtttttctcc gcaaatgtat tggtatcccg ggccgctggg
    38521 aatcctggcc gaatacgcca actcctccca ggaggtgaaa cgcgccacca cgaccatcac
    38581 ccgggcgcag ctcatccacc gggcctggca ggtcgccgtc tcttgcgtcc tgacgggcga
    38641 aagctcttcc tataaagggg tgaagccccg gaccgtgttc gatccggcca aaaaaacctg
    38701 gggggcgctg gagctggtcg gccgatacac cgaattgacg ctggaccggg acgcctttcc
    38761 ccgctatgcc gagcccgcga aatacgccca gcgggcccgc acctggaccg gcggtttcaa
    38821 ctggtatttg aacgacaacg tgaagttttc ttccgactac gattacaccc gcttcaccgg
    38881 gaatcgcgtt cccaaccggt ccgttttctg ccggcttcaa ctcgcctatt aaaaactttc
    38941 ccaaggagac tcgccgtgtt caaaaaaatc gtcccccccc tctcgttgtc gaaacggaac
    39001 atcctttctc tcgcgctgct cccgctgctg ctcggcggaa cggcgacggg cgccgagccg
    39061 gtcatcaaag tgggcgtttc accgaccacc agctcctcgg ctctctacat cgccctggaa
    39121 cagggctatt tcaaagaaca ggggctcaac gtcaagctcg tcacgttccc ctcctcgggg
    39181 gcgcccatga cgatccctct ctcgcagaac cagttgagcg tgggcggagg aaacctcagc
    39241 gcgggcctgt ggaacgccat ccagcagaac ctgggactca aactcgtggc ggacaagggc
    39301 agcgtctcga aggaacacga ctacatcgga cttctggtcc gcaaagacca cgtggacagc
    39361 ggccggtaca aatcgctcca ggacctcaag ggtttcaccc tggggctgac cgcgctgaac
    39421 ggcgtgtccc aggagatcgt gaccgaactg ttcctgaaaa aaggcgggct gacaccgaac
    39481 gacatccggc tggtgaagat gtcctacgcg gagatgaacg tcgccctgcg gacgaagaac
    39541 ctggacgcca ccgtcaacct ggaaccctac ctgacccaag ccgtgctgga cggcttcgcc
    39601 gtgcgcgtgg cgggctccta cgacgtgtat ccggaccagc aaagcgcggc gatcatctat
    39661 tccccggggt tcgcccaaga ccggacgggc gccgccgaaa agttcatggt ggcctacctc
    39721 aaaggggccc gcgattacga ggacgccttc accaaaggca agaacacggc aaggatcatc
    39781 tccctcctgc aaaaacatct gcagatctcc cctgaggccg tctgggagaa actgatcccg
    39841 gccggcatca accccgacgg ccgactcaac ctcgcctccc tggagagaga cctggcctgg
    39901 tacaaggaca aaggttacct gacggacatg cccgacctgc aaaaagccgt ggaccaccgc
    39961 ttcgtcgaaa aagcgctgga gacgctgggc ccctacaagt gatcctcgaa gtcgccggcc
    40021 tcacgaaaag cttcgacgaa cgtcccgtcc tcgacggcat ctccctccag gtcgaggccg
    40081 gcgaattcgt cgtcctcatc ggccccaacg gatgcgggaa gacggtgttc ctgaaaatcc
    40141 tctccggcct catcccgccc tcctccggcc gactccggct cgcgggcccg accaccatgg
    40201 ccttccagcg atcgcctctc ttcccctgga tgacggtccg ggaaaacctc ctcatcagtc
    40261 tcaacgaccc gtcgttccgg ggacgcgcgg aacgggaggt ggccgcctgg ctggaggagt
    40321 cgaacctttc ccgctgggcg gatttctatc cccaccagat ctccggcggc atgagacaaa
    40381 aagtcaacgt catccgcagc ctgatgagca atccgcccct ggtcctcatg gacgagccct
    40441 tcgcgtcgtt ggactatctg gagcgccggc gactgcagac cttcgtctcc cgtctgcaac
    40501 tcgcgaaaaa aacgtcgctg gtctttgtga cccacgacat ccccgaggcg atctttctgg
    40561 cggaccggat cctcgtcatg tcgggaaacg ggaagatcga gacggacctc cgctgtccgc
    40621 tgccgcggcc gcgggaacac gagaccctgc cgcgggaagc cgcctacccg gacctctatg
    40681 aaaaactttc gaaactcctc tccggcaatt aaagggattt ttttcggggc cgtcctcccc
    40741 ctgctgatcc tggcggcctg ggaaatcctc ggccggaccc gggtcgtcaa cc
//
LOCUS       DAOTFD010000003        38955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_697_length_38955_cov_5.084756, whole genome shotgun sequence.
ACCESSION   DAOTFD010000003 DAOTFD010000000
VERSION     DAOTFD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 38955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38955
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_38955_cov_5.084756"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..18293
                     /gene="recJ"
                     /locus_tag="P5079_00445"
     CDS             15..18293
                     /gene="recJ"
                     /locus_tag="P5079_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY28484.1"
                     /translation="MSFAETHDLDITPALELPVPAPEKLSASGLHAGPAAHWVQRLSK
                     LTLAAGLPGGLPERVLPLSAPQIRPLLKTLAEAGGDRVVGELKKFGLGLPEVFSALLS
                     LWVSRHEISAGNAVPAEMEKIFRENAALHKNAGVKNVTVRVFDGAELIEGPDSFILSP
                     FFVDRDGNAVFYMSRLLVEALNRMDGRKRTEILGALAERELLLQQYLRGGMDFDAAHR
                     KILDLAPAQKELFEWASALARTPFVDILTAAPDWKIQHDYLMQAAKKLNLYLKLKSES
                     VSLTEKLFAEGGRPRLYLHELLPRFFGGYLSMRSRKLRAPLESHLPINTEKERTHLAR
                     ELEKGLGRTSGISAFFEGSSQWNFDAYLDRVIAEKVSKNDLTLTVKSVGSARGEEPYS
                     IALMILEKLEDYHKNNAAQLGVSLNKWLARWRVDIQAYDINISNLYATQLGVFDSEQM
                     ASLPDRYRRYFRQEGENFHADDRLRSWIRPQYLDLNDAEQTAHLGRSPADGVFCLRTL
                     LQVPPENRGRIVDDLTAALKPQGHSTYFALTVGVYEESLPYFHFIRDAEGTIVSSPLH
                     KQQAARRDEILSTSKDPAVLERFRLVERAAKLLAEGTDAGRRISPDAILPVDAKDVPA
                     AIITYFENQRRSEALMDGHVPSTVEMYFVLTHPNGDKTYFSQQANRNTEGGPVTERLT
                     RLADWRQDKMIGWGIVRNNLTGNREEPHVGYTHTEENLREEGLGIRRIRSMNAISQKL
                     YALPLASDTTRTSAAEGVWEKLVRRGEARVLDAGTHKKRYVYSVSLPAPKAEPAKTAA
                     SFADEINKLSVYPVTISSQEDRQRTLNAISELLEEIDRPIVFMFDIDQTLMEKNGRPL
                     VRNFKELLRVLRRRNHLVGVVTHRAAVIEKLSGDTDGDAARDITQSAEYAELEQQLAG
                     LGVRIDDLDYLAMGGVVLDENRYIGYVRTQNRFDKNRPGPAQRIYPVDAPWKTSKLFA
                     LSHADDLLADRLGRDNFDGADLVTIGDDLEKDHSTVHMQPHIPKHADRVADFLTQMKG
                     SAVMQKTLAHLRKFTGIIPPNVSVHSKKIGFTNAAGVKDFSLPDDAVEKDAAFFAMEE
                     TILRQLRTLQASKESERVLPLLPVPLSAEPKTPQTAEEFLAQRHGAPRHDGLLARIRS
                     VSEWNVLGETGEFMTVLEHPDVDFILKVPNAATLQNEVQADRILENYELGVERLGGLI
                     PFTAILRNVTLRTEGKPAETYPYVIVQKKIRPLQTVVFEPDQHRRTVREFVSLTHRLW
                     ERGVFDKDMGNVMENYGYDDAGNLVLFDLSHLTDAPDALPVYIENWKSLAPPFYRAFQ
                     FDEVEPHWRRGLDATRRADIPAEEPGLLSLPKALLPFWPQRWVQRHPNRLATIETFLF
                     QGLLVGGVSLVLHAWLGWDLKPLLAATGLFSSLLFTVLHYAVYFDAQAPPVRLGWNPF
                     RHFGVRVKLLALALVLGSAVGIGILVSPVWGGLLGLGTAVLIHLSYLKLPGIVSFLRR
                     LSAEPLHDILSPQKTGWARTLSYISHPASAGLFEAVGLVGVVAVLNPVTPLSWMFVYV
                     GFFFAHPRNMRIFIDSWKKRKLDKEDALTVAAEAFAEALWAATFFRFTSHWITVVAVL
                     YWLSYPQTLKTLYALSKRRIGPRELLYRGIQLIGIFVWIAPYFTPWSLLTGGLISGAF
                     LLFWDLILSPRLGLPILAAIGDDPDKGLLNRWRARQTPRVSPAGARKPDLRSAKRRDV
                     LRYFVGLGMAGAVELLNPQQLFGKVAQPAGMTAKHRILPGEKLSSIAELYGVDEETVR
                     QANRLPGKAALKPGTFLKIPGVSELPAPDDMRALLEGFNVVPTQQAIRLWNAAADIPS
                     KTSAVARAMGWTTVACKSSAYNPWIIYEVKWQIDHAVETGLSPEKLARLKKFYEQVKT
                     NASEVSIQKMTRDLDKPQMPVSAHRGDVWEPLLIVREENGKVVGWDINRFGHVAVNPR
                     KIPIGSLILIEIDGKYTPDGKTIVIPAKAYDTGGHFLSGRLDIDVGYVRARKDTNPKS
                     GTWNRPDIADVVRETPQLAANDLVLPVDGLQIFLPFGAFSFITIGYHQGETLRWYAKK
                     DAIKVHVVKRPLGLFPFWPRAWVQKYPKTLATVETVILGGLVGGVSLLLNHFLGWDLK
                     WVLSGIGLLFALVFSLMHPAVYYDILAPPRSLADMTPGERWRARGALFALGLTMNAAV
                     AAGVLLGPVLGGLLGLAAAGLLHLGYNLRFAPRWSLPLAIFGRKTVSPDRPKEATPPA
                     TISTVETARLLAELLNGIEPDQLIEWAAADARTGRDFDRLAKDIAVRVFEKGPRGVSL
                     GQVLAALAVLHAREGRPIGHVAAALDGNEKDAGLFFALLTDKKPEESAHFLRFLGKTA
                     EVLPPQDRPALEKLMEEFISAQLKKSGDMLLDFVRQTLDEALAAQRQTGEPAVVAFLF
                     TGAEPLYRVAQAIKSSDPKYVSLSLKPLYLTKKIINGHEKPEVFRYLQAQGLFDCRTL
                     ILADTGFLGSIPRYVHRLVRENGLRAPPEIKGRMLCYNTDEEDNRHLAQDQRAGFDIQ
                     GYNTRSRKFPRRTELENFVWFLDMALAHTHNSPEKIAKDRPVLSEMPLPVLRNLGNRL
                     LAQFLGSQRSPLDPEVVYEPPTFLTDPGRLLIDGEKARWLASGMENNIFQHPTQPWVV
                     RVRRLSGADDVKLEAANFALLAKKGLAPAPIARGVTNGHGYLVVEKIEGLSLDRIRRT
                     SPSALEPGLSATRQLLTELLRARLFLQDWKPENVMFGHRPGEPSRALVVDARYLEKTP
                     QLPIRELALRYRQALYGFDGWKTCDPQKKLLAFLNDVIEGHTILEGEEEIFRRGAVAH
                     DISSKTTPVPNEPSVVLVGADNSRDFDAEALTRLESARRNSAEAFSEKLLAEQLARLE
                     KNLSKVNGFSFRWAQAIAERLRAEHSINVDLAPTHFTLASVIQELIKNAFVHGNRLDL
                     SRSVRINWAVENGQLSVTVTDHGTAPVEFTTPPRALDAEFEARLKIASVAGTGEITSN
                     HLGAKLMRKNGYDLRAMPFENAEGRQIGNSVVATIPLTQLSGPVEDPVLARWQARRSQ
                     ETSSRSESSGKSIIDATPHGLFPFWPRTWVRKYPKTLATVETVILGGLVGGLSALLTH
                     YTGWDLKWVLSGVGAATALFFALLHGAVYYDIHAPPRSLADMTPSERWRARGTLFGLG
                     LAMNAAMAAGLLMGPVVGPLLGLAAAILLHIGYNQWLAPRAGLPLGTIGGESWRDDYE
                     APLSRSFEWFINQAKELVLPAKTREKFHETGKAKDFPGLSAVLTFKKGSPTHDRVRTL
                     MGQLKGDLERAGVSHYFAFLDAADYEPHMTLYDTALRPDRPLTDELTRKVKDAFTGLA
                     QENLVPAEWSAAGCAISAGEGGASIVLRTHPKQKQDLDTALRVRDRLHAALRSPDRGV
                     GTDVPFIGHTTLAYIVKDIPPADYRKVQDILRRYVFQSAGNFLFDRVEFRKFNTMLDW
                     DDPLAELTLQPGSAVPQKPKKKWLIHDTGAAPIAEIAGFLSVTGYPQLIARELTEAMN
                     RRLAEGKKVALLSPEVGRLLMEYGLWTGDAAMNFFGRFEKADKAVIFTALKKADIPVL
                     KRYFQKDHPPKPDLSRSIFEVLAKILARRKNDTVEKVARFLNADGRQKESDYNDLPHM
                     REAVKLIKDAVKKQEKIVIFGDYDVDGVTATAIMLKTLHRILKAEGIPPEDYGKYLGY
                     HIPNRFTEGYGMSVAGVDEVIAEGAKLLITVDNGIRCRKPVERALELGIRVIVTDHHQ
                     PDKENADTPVNVPLIHAMYIEERDHPAKHISGAGVAYKLAQALMDDYGIQRPADDLLD
                     LFAFGAVADLVPLRGDNRLLVTRGLELINSPRVSLGIRKLLEVARKNGKIKEDQLVET
                     GTIGYYLAPMINASGRIASATEALDLLMTEDAAMADEYAGRLYELNMERREIEAQMVE
                     LVETRLKKEFDSQTKGAIVVGEEGWNEGVIGILASRIVETWGLPAAVFSIDREKGIAK
                     GSMRTIPGVSVIDALTECQKQGLVLKGGGHEAAGGCSVALDKFEEFKRAYSDAVRRQM
                     ETKNIEPFIELDALLATEDMGIPLIQLLEKLAPFGVGNARPVFAGRGLRVTSYKISKD
                     GKHVRGIRLDSPNRSLEAVAWRMATPEILQLLNDSVRTGKKLDLIFTLETSDFGGVHP
                     QLVLRDIRPASETSPAETARLAKAVVAGGPLRPVTPPRERLEKILTRAGNGDLPSDDI
                     DDILQAWEGLRVYKYKTLEKESVDFFIRLLERLKKLPDVQREEILDRLHPLFETMLDD
                     QSEDWRQSFLNLRSPKDQPRPLWAQPLDFNALFDDTDVLLFGDTHTMISQRAFLAQRM
                     RELKEAGVTHFVYEGVGRQVNNRVREDRRVTLIKGDNIATQMLRRAAEEAGIQFVPID
                     MSAQLMDSILRKLSSFASARLGRSYDFETLKQYLTTDTVYDELVMRRRNIYFSNFIKT
                     ILRNNPNAKIAGIMGETHLTSKRVRGGATGVVERLRSAGIHAKGVRLENAESAEAPLF
                     DGTRWDAPGRGSTVYIPRDMTYWGRYILDTFLTPPATPPEKTPARKDGGSLADRISRA
                     SWRRVSEPSLVGHEVEVFVSDELGLVVKVPRPKNKKKWNTVVKPHAQTAIERLGPEAA
                     NAAPLAPPTVLLKNIQLTVDGQTLRFDEGIAQARVPLLGEVMHELDKTGDLAGTTALL
                     NQLIGLLTDLARRGLIYNDEFVLKNFGVWRGKDGRLSVVVIDFGALTPGQGLLDKDPD
                     LIYFYPAYVDSLKDNLKGWHTPYGKFRYAEKLIACLDAEKYFLTLNHLLGELGGLAKP
                     LAPPTPSSPQKAARPFDVSHNATAWLAGAVALSLGLLLTVFGAAFVAHTADPAAFWVS
                     LLVLGWGQLFTWNGQAYLRRYYADWKKANSFRAGTTTRPAPSEETTPIDEELWQSYVA
                     GLKKMNPDFRAEDLRVDWDGQKTRGSRLASTDEDGNVWLDPWLVTDVRQWVTAQNRTA
                     LEQSDVRLLLLQKELLPLIVQHEGLRVRYTQHPLLQIPLLQTLWIYLTKPAPEPIDVA
                     AASAKKEPTRLERVLQDIRLFRKNFTRLESLLAADTQGDPLHIHGHVANGLALLSSLD
                     GEPVTRPVEEWEDLRDRLARTLNDIERRVGASMFPRSGRPESELQQKLEWLKNDAGIT
                     SETTAESRRTYERYLSNRQSDPLQLGITGGLYGGNTRLLLARRLGLEPYRIPVTTVVR
                     GGEQPETLLTAAVAGRLDAHRRESQITDADRAARLGALVSRLLRNLVESGYMSAADSR
                     DFVTAEKSESWRRSLAADNLDPVKHAVFIEGALWQRLYGAVRDRAPGVSLSSDELTAA
                     LDTAMMRLTAKDEKKAATTAAKPVLPTTPEKPAAPSERTVETPSFDLRSGGFPALLHG
                     LGVWTRLARATRLVYAGLTLGVSPVQLARAESMAYALAETAAKAMLTAKTGIKTEQKA
                     DGTLVTEMDKRIQDLAAAEIRRRFPRHTIFAEEGAAQRTVSEWMWTIDPINGTKAYAE
                     GGTEYTFDMSLLHNGRPVLAVSFAPEMTAAGQPGVRAIGSRWTGTLLNGKPVRFDRSQ
                     DGKRDWDVISRSSVSRKKDDAYLRTWRDLYGESHVRQVSNGALAGLQILTEQNDAVFS
                     NVVAEPWDPVAISALLEWAGGAPLRYDGASYFPLTPADFDRAGQGSSGYILTRGGIHG
                     KRAAEAASLSNGLPEPANTPIDIGVDIQLAVPPVQRPLSEIKDSDVLVARLLPLAGDS
                     SASSSSPSLLEALTLSSEKTAAGTGNRLPLERLSPEAARRLLPYMTDAERRAYGATLR
                     REAKNIRDSLSAGRADEGAFRELLRNVGRVHGQGGVLFMPANAADIPRQKRREMGHLV
                     RLAYESGLIPAGAVDGVAAQKLQGYMDVYNRARHTAEMRTNRSAVDRLRESASSLLRK
                     LRELVLGDASLRVFHIPVSRDAAGKLLLLDDARTGLWLDRLSRTLSTEGQKVRTVLVT
                     NESLSSNEKGELWDLVVAALARRTASAAEAAQIAARLKASLATDRPILFREDVGDETR
                     PGIIQARSLSKYLKGVPAGAAMDIYTLWEDQWDLSGLRKEAVRLLVLFSGDILFNAAE
                     SLEKSIATIRYIQIQA"
     gene            18353..19648
                     /locus_tag="P5079_00450"
     CDS             18353..19648
                     /locus_tag="P5079_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRY28485.1"
                     /translation="MNRIYFPEERIPLVKLLPMGMQHVVAMFGATVLAPILMGFDPQV
                     ALFFSGVGTLLFLLITRFKVPSYLGSSFAFIGPVLAVTSGRPENIPFALFGIAGAAVL
                     YAAAAGITLRWGSSWIDRLMPPIVTGTVVSIIGLNLASSSVANALNADFAIRTGADAI
                     RLTVAAATFLTAAFVSIYMKGFLRLLPILTGVVVGYIVAAVAGILDPAALQNVREAAW
                     IGLPPFQTPRFSVQALLVIAPVFVVLVAENKGHIAAISGYMGRDLNPHLGRAYLGDAV
                     ASFVSALGGGTPQTTYAENMGVMAITRVFSTYNFLMAAGIAVLLGLCPKFGAVIQSIP
                     APVLGGVTLILYGLIALMGVKIWTDAKVDFCDHRNLVVAGSSLIVATGLGVRGLTVGG
                     VNVAGIAFGTLLALAANFLLNLQKPSPRAAEDVPDATSI"
     gene            19720..19899
                     /locus_tag="P5079_00455"
     CDS             19720..19899
                     /locus_tag="P5079_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28486.1"
                     /translation="MKIRMIALLALASLSLVAGFGCKKEAPMEEQPMQEQAAAPEAAA
                     PEAAPEAPAEAPQGN"
     gene            20128..21639
                     /locus_tag="P5079_00460"
     CDS             20128..21639
                     /locus_tag="P5079_00460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRY28487.1"
                     /translation="MLFNSFEFLIFFPAVTLLYFALPHRFRWALLLAASCLFYMAFIP
                     QYILILFLTIAVDYTAGLYIEKSEGHRRKMFLAVSLVCTAAILFVFKYFNFFNSNFAR
                     IAEFFHWNYPIQGLSIILPIGLSFHTFQSMSYVIEVYRGRQRAEKNFGIFALYVMFYP
                     QLVAGPIERPQNLLHQFYEEHRFDYRRVTDGLKLMAWGLFKKVVIADRLAVFVNHVYA
                     DPASFPGISLLLATVFFAFQIYCDFSGYSDIAIGSAQVMGFRLMDNFNRPYFSKSVAE
                     FWKRWHISLSSWFRDYLYIPLGGSRVSVPRWYLNLFLTFLISGLWHGANWTYVFWGAL
                     NGAYLVSEIVTAGPRRRFVQAVGLDRLPRLHKIVQVSITFSLICFSWIFFRAENLREA
                     FYIVGHIFSGVGPFVKNFTLYLLNVNFKLGLTVDSAVRDQLLMGQTMPEFRRALLAIG
                     FMEIVHLIQRHKNIRHFLSQKPFLARWTVYYLLVISILLWGLFKDEKFIYFQF"
     gene            21611..22627
                     /locus_tag="P5079_00465"
     CDS             21611..22627
                     /locus_tag="P5079_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28488.1"
                     /translation="MKNSSTSSSDVRRPLLLFAAKLALMAALAAATCLATFVLPLPYD
                     QNLAVIINKRDRLRNMPGERLIFVGGSGLFGGLDVRRLEAELKKPVANLGFFAGFGIF
                     HLLDEVTPFLRPGDTVLIVPEYGLIRQDFQNNEQSRKWILRLSPGRHALQLYPPSKDG
                     LVNFVQDSGALLLSQLATLRKAVETLFKRDTRFYTPRGFVKYRLITDETGEPTEHHRF
                     AVVPADQVAGKGDTYPDLTVKPESMALLNNFGEAAARRGARVLFVFAAFPREEYELNK
                     AAIDRLHRQLKNELRFDIVGQPEDFIYPGPLFANSIDHLNADGVRERTDKLLALLRPH
                     LQNK"
     gene            complement(22709..23716)
                     /locus_tag="P5079_00470"
     CDS             complement(22709..23716)
                     /locus_tag="P5079_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HRY28489.1"
                     /translation="MSEKTEKKILAILKVLHESVRPLSSSAITEHLLAMGHDISERTV
                     RFHLLAMDEEGLTKSFGKRGRQITEKGSRELSTARVIEKVGYLAAKIDQMTYRMNFNL
                     DNRTGGVVINISIIETKRLQENIPLLSRVFEEGYAMGHLVALFPQGEKIGDIVVPPGH
                     VGLGTVCSITINGVLLAHGIPTTSRFGGLLELRDRKPVRFAELIHYEGTTLDPLEIFI
                     RSGMTDYTGAVKTGSGRIGASFREIPADSRERVIELAKRLEEAGLGGFMTVGWPGQPL
                     LEIPVGEGRAGAVVIGGLNPVAILVENDIRVHSRALSTLVDYRTLFHYKELDGRLRRL
                     R"
     gene            24074..24925
                     /locus_tag="P5079_00475"
     CDS             24074..24925
                     /locus_tag="P5079_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRY28490.1"
                     /translation="MRIIEKNTLNDTQGMRLTRMRFEAPAIARKALPGQFILLMVHEK
                     GERIPLTIVETDAAAGTLTLIFQEVGHTTKLLGKKNVGDSLYSLVGPLGHPSEIPRNE
                     RVILVGGGVGIAEILPVARAVKAAGNHVVSVLGARTKDLLLLTREVGETSDRLLICTD
                     DGSAGEKGLVTDVLKKLLAEPEPTHRIFCVGPVPMMRAVSETTRPSGIKTIVCLNTIM
                     MDGTGMCGGCRLIEGGKARFCCVDGPDFDGHAVDFADLVQRQRRFREEEMKSLDHACR
                     LEGTAAP"
     gene            24922..26322
                     /gene="gltA"
                     /locus_tag="P5079_00480"
     CDS             24922..26322
                     /gene="gltA"
                     /locus_tag="P5079_00480"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRY28491.1"
                     /translation="MSTPSRPRPEPGRIHKISMDERKTSFTEVSRGFPPEEAVKEAAR
                     CLQCKNAPCSKGCPAGIDVREFVRFIREQNFQSSIDKIRESNSLPGVCGRVCPQEEQC
                     QKACVFNAKKIPIQIGYLERFVADWELAHGKRQPARSAAPKEKVAVIGAGPAGLTCAA
                     DLVREGFDVHLFEALHVAGGVLMYGIPEFRLPKDIVKKEADYIGSMGVKIETDAVVGR
                     TYTIDELRRDGYKAFFIGVGAGLPILLNIPGENANGVYSANEFLTRVNLMKGYRFPEY
                     DTPVRVGKRVGVLGGGNVALDCARSALRLGAESVTLLYRRTRDEMPARQEEIENTEEE
                     GIHLQFLVTPREILSDDKGNVRGLRLLKNVLGEPDASGRRSPVPQPGTDFELPLDTVI
                     VAVGTAVNPLLLSTMEGLERTKKGYIKVGEDMRTSLPDVFAGGDIVSGSATVILAIQQ
                     ARTAARAITHYLRNGK"
     gene            26375..27703
                     /gene="gdhA"
                     /locus_tag="P5079_00485"
     CDS             26375..27703
                     /gene="gdhA"
                     /locus_tag="P5079_00485"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRY28492.1"
                     /translation="MHTAFMKMVVEKNPGETEFHQAVEEVVTSLLPFIEKNPQYKHAK
                     ILERIVEPERVLMFRVPWVNEKGEVQVNRGFRVQMNSAIGPYKGGLRFHPSVNLGILK
                     FLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDTDVMWFCQSFMTELCKHIGPDTDVP
                     AGDIGVGGREVGYLFGQYKRMRGEFTGVLTGKGLGWGGSLIRPEATGYGCVYFAQEML
                     KTLHKAVGGHVCAVSGSGNVAQYTVEKLNELGAKVVTLSDSSGFIHDPDGVDEKKLAF
                     VMELKNLRRGRIKEYADKFKCKFYEGKRPWGVKCDMAFPCATQNEIDKTDAETLLKNG
                     CLCVAEGANMPSTLDAVAAFVKAKILYAPGKAANAGGVATSGLEMSQNSLRLSWTRQE
                     VDKKLHDIMINIHESCVKYGQEGKYVNYVKGANIAGFVKVADAMLAQGVV"
     gene            complement(27966..28331)
                     /locus_tag="P5079_00490"
     CDS             complement(27966..28331)
                     /locus_tag="P5079_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28493.1"
                     /translation="MAKILIAEDDESTRELLKYIVMSAGFEVTTAADGQIALNMITNA
                     PPDLVVLDIMLPEVHGFSICHQVKSNEALKKIKVLMLSAKAFPADRRQAENVGADGFM
                     SKPVNPEELIQKIKSLLSA"
     gene            28444..31242
                     /locus_tag="P5079_00495"
     CDS             28444..31242
                     /locus_tag="P5079_00495"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY28494.1"
                     /translation="MIELPSIYNPEQTESKRYDVWMKHGLFGSQPDPKRSPFTIVIPP
                     PNVTGSLHMGHALNNTLQDVLIRWHKMKGDNTLWIPGTDHGGIATQNVVEKMLKKEGK
                     TRHDLGRETFLKRMWRWRQDTGDTILHQLRKIGCACDWDRTRFTMDEASSKAVMTAFV
                     ELYRRHLIYRGRRLVNWCPRCFTALADVELDYVESAGHLWHIRYPFADGSGRTLVVAT
                     TRPETMLGDTAVAVHPDDKRYADARGKTLRLPLMDREIPIVFDAAVDPAFGTGAVKVT
                     PSHDAADFEIARRQNLPHEMVVGYDGRMTDRAGKYAGLSLKECREKVLADLTAGDFLE
                     KEEPYKHSVATCYRCATVIEPLESSQWFMKTGDLAKRAAHATEVDRVQIFPESWEKPY
                     IHWLTNNRDWCLSRQIWWGHRIPVWYCLRCSRLNEDQLGQIARDPTFQLESTKMILRE
                     SASDPIVREEDPVVCPRCGKEEIIQDPDVLDTWFSSALWPISTLGWPKKTQDLSYYYP
                     TSVLVTGHEILYLWVARMVMMGEELASEVPFRHVFIHGIVRDKHGKKMSKSLNNVIDP
                     LDIMKKFGTDALRFALISSAIPGRDMQLSDDSFVGARNFANKIWNASRFVLTNLSDHR
                     RTGLPPDKMDLSDRWIRRRCVETLKNVDAFVEGYDLAQAARTLYSFFWGEFCDWYIEL
                     AKIRLQGLGGPAENPPPKPPTTDAALAAKETLATVLDSVLRALHPIMPFITEELWGAL
                     GETLGEKTEGCLMAAPGRVPALFDSPAASEADMRAMEVLMETVTALRTVRSEMNVPPG
                     KSVPLLVNAQNALPFNKGLLETYGDYVKRLGKVSEIRFIGKGEKPAQAAAAVASDFEL
                     FIPLEGLIDFEKEKQRLSKEVVSLDADLQRLQARLSNPDFTARAPQEEVTKAKDRHTE
                     VTAKKQRLQTHLAALAKD"
     gene            complement(31239..32696)
                     /locus_tag="P5079_00500"
     CDS             complement(31239..32696)
                     /locus_tag="P5079_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28495.1"
                     /translation="MPPVLPRPIVKPWPLLWGTVVVLAVFAGRLWRISSAMGTPSYYY
                     TDSSSHILNALWHATGVLKWSFPRVPLAGFLYAAATRMGLDVFYAAWAVNAALGLMAV
                     WLLFDAVRRLAGPGAATVSAVLALSVPSFGRLVFSGQTEMTALAFTALSFWIACYGLF
                     LDEPSNFWADLAAFCLLGYAASVRSDGLGLFGLAGIFLFLRWRRRPRFWPFFVGAGLV
                     CLYVILPSLMVHGRGASTANAADQWLSFGQRFLRLPAWLLKESFPFNVFLCAGLAHFS
                     GKRFFRPLGWFAVLVLGQAFLLWAGSWFYLFSWETERNMNTVLFLAMAPAGAAVWAAA
                     SALGRYGMLLGLLLAAGPVGWSWERAMKEVPARLSLEDHNRGLGRRLRELNAEGKLGR
                     PESLILLEVADSGWPNVLVFSGMPERFRLLPSTETEWGKVRIWDGRGEAGVAVVSSER
                     AEKELAEDGGSFERVDVFGDYNILRVGKAPAAPGN"
     gene            complement(32712..33602)
                     /locus_tag="P5079_00505"
     CDS             complement(32712..33602)
                     /locus_tag="P5079_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRY28496.1"
                     /translation="MSLLDSFKSSNFAPAFFFLSAPRRRALQAVYAFCRSVDDTVDDE
                     VLRRARGERPSDGPQRTLAAWRELLRALRDGRTAVESRKEGTLEQSAAQVDAAVWPAL
                     LETLERYPTAAAPLLGLVDGVERDLLKNRYGTFEELKEYCYGVAGTVGLACLPVFGLD
                     EESHREFAVTLGRAVQLTNILRDIPADAAVDHVYLPAEDLNRFGYGEEELKKSVYNDN
                     FVRLMRFEASRAEEAYAAALQKLPDTSRRAARPALIMARLYRALLGEIKKRGYNVFLG
                     RARLSTYQKMRVLPGIFWNA"
     gene            complement(33612..34535)
                     /gene="hpnC"
                     /locus_tag="P5079_00510"
     CDS             complement(33612..34535)
                     /gene="hpnC"
                     /locus_tag="P5079_00510"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HRY28497.1"
                     /translation="MTPLPASTQLASSALAAASLPEAYGACLSLCRSHYENFPVASRM
                     IPAEQRGPIAAVYAFARVADDFADEPGWTPPERLSLLDDWRRRLRACGSSHNRHPVFW
                     AVADAVERFRLPLDPFERLITAFERDVTKNRHENFDEVLDYCRYSANPVGELVLRVFG
                     FWNEERGKWSDAVCTGLQLANFWQDVTVDAAKDRIYVPREDMKAHGLAEEQILRGPVI
                     EPLRRLIAFQVERTWTFFRAGVPLCDDVPNPLRRELRLVWLGGTRILEKIGAQDGDVW
                     GQRPTLGKTDLPRLMQRLFFWNPAAVLPRGS"
     gene            complement(34724..34966)
                     /locus_tag="P5079_00515"
                     /pseudo
     CDS             complement(34724..34966)
                     /locus_tag="P5079_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
     gene            complement(35074..35382)
                     /locus_tag="P5079_00520"
     CDS             complement(35074..35382)
                     /locus_tag="P5079_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRY28498.1"
                     /translation="MAFGKILSKLTPAKGEMAGTLGRNDPCHCGSGKKYKKCHLPQDE
                     EAERKQLAKAEIPPKPPESEKPGAKPPHDKHPESGRPAHEKSGGGFMRSIFNRKTGGG
                     "
     gene            complement(35405..35767)
                     /locus_tag="P5079_00525"
     CDS             complement(35405..35767)
                     /locus_tag="P5079_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28499.1"
                     /translation="MPRLTRKKLDHFLSSLCEEAGMSGKPSGNKTAGGSHGLVCRAPC
                     RKADWRAHLDIPAGILCVSAAWSANKQAAKHRDVTIAITFLDDDGKPDYADIVFCDSE
                     IRWLMETLWHAPLWDEDR"
     gene            36040..36987
                     /locus_tag="P5079_00530"
     CDS             36040..36987
                     /locus_tag="P5079_00530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY28500.1"
                     /translation="MTLSFWHRWRKEFARRLTIMVIPHGIARPRQLSFSVSFLLFLFL
                     CWTGVTAWAGYMASQRFDYWRLKANTHVLRIKVEYFANEVKRAREMLDDVKEMETQLR
                     TMMGMGSRDAIIQSEENPLQNQGGPTAGDEMELQKLLDGRVSELTLQDVSLQIHQLRQ
                     EMDQRLSSFSELTEKIDYERRLFRSLPNIWPTNGYLTSHFGHRLSPFSGLDDWHKGVD
                     IAAPPGSPVHASADGVVILAGWAGGYGKVVVVDHGYGYSTRYGHNRQILVKRGDRVRR
                     GQILSLVGSTGNATGPHCHYEVWFLGRPVNPRRFMHLEG"
     gene            37033..37395
                     /locus_tag="P5079_00535"
     CDS             37033..37395
                     /locus_tag="P5079_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY28501.1"
                     /translation="METVLGMETHFQGTIRSKGSIRVDGRVEGGISAEGVVIGESGQV
                     QGDITAKNIVIGGKVTGNLIASAVLELQAHSQVFGDLRTAQLSIAEGAIFEGNCVMTT
                     EKAKVIAMDEVMAKNSRG"
     gene            37502..38221
                     /locus_tag="P5079_00540"
     CDS             37502..38221
                     /locus_tag="P5079_00540"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRY28502.1"
                     /translation="MMKWLRKHRYTIFLITVGGFLVGSFMGFGSYFFTQSPYDAAIVV
                     NGDKIPYKRYVTRYHQYLNQRRDNNEPLNEENIKRIKQETLQDLVREAVFLQEADKYG
                     LTVTDNEVAAYIQRSPAFQRDGQFDQGAYLQVLTQVLRIPADEFEDDRRREIKIQKLQ
                     ALMASAVKVSNIEFNWQYQKRMALGNAEEKKTAKDKPNEFREQLRQEQVSHVFQEWLS
                     QINSQLKVKVYLDKWEGRGEG"
     gene            complement(38282..38890)
                     /locus_tag="P5079_00545"
     CDS             complement(38282..38890)
                     /locus_tag="P5079_00545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRY28503.1"
                     /translation="MPFVLASASPRRRRLMATFPFPVHVRPGRVKEPRWKRGVRPSVW
                     VKRLALLKAAPAAAAVSEGLILGADTLVFRRGRVFGKPSSVAAARRMLAELAGRWHTV
                     YTGLALLARPGRKHWECVWSTRVKMRALSPERLRYWSQRNHDKAGAYAAQEKGSPFVE
                     KLRGDYDNVVGLPRRGLRRLLAKARSAGYAPSRSAGRSRKGI"
BASE COUNT         7939 a        12981 c        11098 g         6937 t
ORIGIN      
        1 tgctcagagc cgttgtgtcc ttcgccgaaa cccacgacct ggacataacc cccgcgctgg
       61 agttgcccgt ccccgcgccc gagaagcttt ccgcctcggg tctgcacgcc ggccccgccg
      121 ctcactgggt tcagcgtctg tcgaaactga ctttggcggc gggccttccc ggcggcctcc
      181 cggaacgcgt ccttccgctg tccgccccgc aaatccgccc cctcttgaaa accctggccg
      241 aggccggcgg agaccgggtc gtcggggaac tgaaaaaatt cggcttgggc ctgccggagg
      301 ttttctcagc ccttctctcc ctgtgggttt cccggcacga aatctccgcc ggcaacgccg
      361 tgccggcgga aatggaaaaa atattccggg aaaacgccgc cctccataaa aacgcgggtg
      421 tcaaaaacgt tactgtccgc gtcttcgacg gcgccgaact gatcgaaggg ccggacagct
      481 tcatcctctc ccccttcttt gtggaccggg acggcaacgc ggtcttctat atgagccggc
      541 tgctggtgga ggcgctgaat cgaatggacg gccggaaacg caccgaaatc cttggcgccc
      601 tggccgaacg ggaactgctc ctgcaacaat acctgcgcgg cggaatggat ttcgatgcgg
      661 cccaccggaa aatactcgac ctcgccccgg cgcagaaaga gctcttcgaa tgggcctccg
      721 ccctcgcccg cacgccgttc gtcgacattc tcaccgccgc tcccgactgg aaaatccaac
      781 acgattattt gatgcaggcg gccaaaaaat taaacctcta tctgaaactg aaatcggagt
      841 cggtttcgct gacagaaaaa ctctttgcgg aaggcggacg gccgcggctc tatcttcatg
      901 aacttttgcc gcgtttcttt ggcgggtatc tttccatgcg atcccgaaaa ctgcgcgccc
      961 ctctggaatc ccatctcccc atcaacacag aaaaagaacg cacccatctc gcgcgcgaac
     1021 tggagaaggg gctggggcgg acttccggca tctcggcgtt cttcgaaggg tcgagccaat
     1081 ggaatttcga cgcctacctc gaccgcgtga tcgccgaaaa ggtctcgaaa aacgacctta
     1141 cgctcaccgt caaaagcgtt gggagcgccc ggggcgagga gccctattcc atcgccttga
     1201 tgatcctgga aaagttggag gattaccaca agaacaacgc cgcccaactc ggggtttccc
     1261 tcaacaaatg gctggcccgc tggcgggtgg acatccaggc ctacgacatc aacatctcca
     1321 acctctacgc gacgcagctc ggtgtgttcg attcggaaca gatggcgtcc ctgccggacc
     1381 gataccgccg ctacttccgc caggagggtg aaaatttcca cgccgacgac cggctgcgga
     1441 gctggatccg gccccagtac ctcgatttga acgacgccga gcagacggcc cacctgggcc
     1501 ggtctcccgc ggacggcgta ttttgtctgc gcaccctcct gcaagtcccg ccggaaaatc
     1561 gcggccggat tgtggacgat ctcacggccg cccttaaacc acaaggccac tccacctatt
     1621 tcgccctgac ggtgggcgtc tatgaagaat cgctgcctta cttccatttc atccgcgacg
     1681 ccgagggaac catcgtttcc tctccgctgc acaaacaaca agcggctcgc cgagacgaga
     1741 tcctttcaac ctcgaaagac ccggcggtcc tggaacgatt ccgactggtc gagcgggcgg
     1801 ccaagctgct ggccgaaggg acggacgccg ggcggcggat ttcaccggac gccattcttc
     1861 ccgtcgacgc caaggacgtg ccggcggcga tcatcacgta tttcgagaac cagcgccggt
     1921 cggaagcgct gatggacggc cacgttccga gcaccgttga aatgtatttc gtcctcacgc
     1981 accccaacgg ggataaaacg tacttctccc aacaggcaaa cagaaacacc gagggcggcc
     2041 ccgtcacgga acgactgacc cgtctggcgg attggcgcca ggacaagatg atcggatggg
     2101 gcatcgtgcg gaacaatctc accggaaacc gggaggaacc ccacgtcggc tacacccaca
     2161 cggaagaaaa tctccgggaa gaggggctgg gaatccgacg gatccgttcc atgaacgcca
     2221 tctctcaaaa gttgtatgcc ctgcccctcg cctccgacac gaccaggact tcagcggcgg
     2281 agggcgtctg ggaaaaattg gtccggcggg gagaggcccg ggtcctcgac gcgggaacgc
     2341 acaagaaacg ctacgtgtat tccgtctccc tccccgcgcc gaaagccgaa cccgcgaaaa
     2401 cagccgcctc gttcgcggac gagatcaaca aactttcggt ttacccggtc accatctctt
     2461 ctcaagaaga ccgccaaaga accctgaacg ccatttccga acttctggag gaaatcgacc
     2521 gacccattgt gttcatgttc gacatcgacc aaacgctgat ggaaaaaaac ggccggccgc
     2581 tggttcggaa tttcaaggaa ttgctccggg tcttacgccg ccggaaccac ctcgtcggcg
     2641 tcgtcaccca tcgcgccgcc gtcattgaaa aattgtccgg cgacactgac ggagacgccg
     2701 cccgggacat cacacaaagc gccgaatacg cggaactcga gcaacagctg gccggactgg
     2761 gcgtccggat agacgacctc gattatctcg ccatgggcgg cgtcgttctc gatgaaaatc
     2821 gctacatcgg ttacgtacgg acgcaaaacc ggttcgataa aaaccggccg gggcccgccc
     2881 aaaggattta ccccgtcgac gccccctgga aaaccagcaa gctcttcgcc ctgtcgcacg
     2941 cggacgatct tctggcggac cggctgggcc gcgacaattt cgacggcgcc gacctcgtga
     3001 ccatcggcga cgacctcgaa aaagaccatt ccaccgtcca catgcagccc cacatcccaa
     3061 aacacgccga ccgggtggcg gatttcttaa ctcaaatgaa gggctctgcc gtgatgcaaa
     3121 aaacgctggc ccacctccgg aaattcacgg ggatcatccc ccccaacgtg tccgtgcaca
     3181 gcaaaaagat tgggttcacc aacgcggcgg gggtcaagga tttctccctg cccgacgacg
     3241 cggtggaaaa ggacgccgcc tttttcgcca tggaagaaac catcctccgg caactccgaa
     3301 ccctccaggc atcgaaagaa tcggagcggg tcctccctct tctcccggtg cctctctcgg
     3361 ccgaaccgaa aaccccccag accgccgaag aattcctggc gcagcggcat ggcgccccga
     3421 ggcacgacgg ccttctggcc cggatccggt ccgtgtccga atggaatgtc ctcggcgaaa
     3481 ccggggaatt tatgacggtt ctggaacacc ccgacgttga cttcatcctc aaagtcccca
     3541 acgccgcgac cttgcagaac gaggtccagg cggaccgcat cctcgaaaat tatgaactgg
     3601 gcgtggagcg cctgggcgga ctgatcccgt tcaccgcgat cctgcgcaac gtgacgctcc
     3661 gcacggaagg caaaccggca gagacctacc cctacgtcat cgtgcaaaag aagatccggc
     3721 cactgcagac cgtcgtgttc gagcccgatc aacaccggag gaccgtccga gagttcgtct
     3781 cgctgaccca tcgtctctgg gaacgcggcg tgttcgacaa ggacatgggc aacgtcatgg
     3841 agaactacgg ctacgacgac gccgggaacc tggtcctgtt cgatctcagc cacctcaccg
     3901 acgcgccgga cgcgctcccg gtgtatatcg aaaactggaa atcgctggcg ccgccgtttt
     3961 atcgggcttt ccaatttgac gaagtggaac cccattggag gcgcggcctc gacgcgacgc
     4021 ggcgcgcgga tatcccggcc gaagaaccgg gcctcctctc gcttcccaaa gcgctcctgc
     4081 cgttctggcc ccaacgatgg gtccagcgcc atcccaaccg cctggcgacc atagagacgt
     4141 tcctcttcca ggggctcctg gtgggtggag tttcccttgt cctgcatgcg tggctggggt
     4201 gggacctgaa gcccctcctg gccgccacgg ggctgttcag ctcgctgctt tttaccgttc
     4261 tgcattacgc ggtttatttc gacgcccagg cgccgccggt ccgactcgga tggaacccgt
     4321 tccgtcattt cggcgtccgc gtgaaactcc tggcgctggc gctggtcctg ggatccgccg
     4381 tcgggatcgg gatcctcgtt tcgcccgtct ggggcggcct gctgggactc gggacagccg
     4441 tcctgatcca tctctcctat ttgaaactcc ccggcatcgt ttctttcctc cgtcgactgt
     4501 cggcggaacc cctgcacgat atcctgtccc ctcaaaaaac agggtgggcg cggacgcttt
     4561 cttatatctc gcatcccgcc tccgccggcc tcttcgaggc cgtcgggctc gttggcgtcg
     4621 tggccgtgct gaaccccgtg acccctctct cctggatgtt cgtgtacgtc ggctttttct
     4681 tcgcccaccc ccggaacatg cgtatcttca tcgactcctg gaaaaaacgg aaactcgaca
     4741 aggaagacgc cctcaccgtg gcggcggagg cgttcgcgga ggccctttgg gcggcgacgt
     4801 tcttccggtt cacgtcccat tggatcaccg tggtggccgt gctttactgg ctgtcctacc
     4861 cccaaacgct caaaacgctc tatgccctca gcaaacgccg catcggacca cgggaacttc
     4921 tttacagggg aatccagttg atcggaattt tcgtctggat cgcgccttat ttcaccccat
     4981 ggtccctgtt gaccggcggg ctgatcagcg gcgcgttcct gttgttctgg gacctgatct
     5041 tgtccccgcg gctgggccta ccgatcctgg ccgccatcgg cgacgacccg gacaaaggcc
     5101 tcctgaaccg ctggagggcc cgtcaaacgc cgcgcgtttc tcccgccggc gctcggaaac
     5161 cggacctccg cagcgccaaa cggcgcgacg tgctccgata cttcgtcggg ctgggaatgg
     5221 ccggggccgt ggaattgctc aacccgcaac aattgtttgg gaaagtggcg cagccggccg
     5281 ggatgacggc gaaacatcgg atcctgcccg gcgaaaaact ctcctccatc gccgaactgt
     5341 acggcgttga cgaagaaacc gtccggcagg ccaaccggct ccccggcaaa gccgccctga
     5401 aacccggaac gttccttaaa atccccggcg tgtcggaact gccggcgccg gacgacatgc
     5461 gggccctcct ggaaggattc aacgtcgttc cgacgcagca ggccatccgg ctgtggaacg
     5521 ccgcggcgga catcccctcc aagacctcgg ccgtggcccg ggccatgggt tggacgaccg
     5581 tggcctgcaa aagttcggcc tacaatccct ggatcatcta cgaggtgaag tggcagatcg
     5641 accacgccgt cgagaccggc ctgagcccgg aaaaactggc gcgcctcaaa aaattctacg
     5701 agcaggtcaa aacgaacgct tccgaggtga gcatccaaaa aatgacccgg gacctcgaca
     5761 agccccagat gcccgtctcc gcccaccggg gggacgtctg ggaaccgctc ctcatcgtgc
     5821 gcgaagagaa cgggaaagtc gtggggtggg acatcaaccg cttcggccac gtggccgtca
     5881 acccgcggaa aatccccatc ggttccctca tcctgattga gatcgacggt aaatacacgc
     5941 cggacgggaa gaccatcgtc atcccggcca aggcctacga cacgggcggt catttcctga
     6001 gcggccggct ggacatcgac gtgggctacg tgcgcgcccg aaaagacacc aaccccaaat
     6061 ccgggacctg gaatcggcca gacatcgcgg atgtggtgcg agaaacgccc caactggccg
     6121 caaacgacct ggtactgccg gtggacggcc tgcaaatctt cctccccttc ggggcttttt
     6181 ccttcatcac catcggctac caccaagggg agactctgcg ctggtacgcc aaaaaggacg
     6241 ccatcaaggt ccacgtggtg aaacggccgc tcggcctgtt ccccttctgg ccgcgggcgt
     6301 gggtgcaaaa atatcccaaa acgctcgcca ccgtcgaaac cgtgatcttg ggcggcctcg
     6361 tcggcggcgt ctctctcctc ctcaaccact tcctcggctg ggacctgaaa tgggtgctga
     6421 gcggcatcgg gctccttttc gccctggtct tctcgctgat gcaccctgcg gtttattacg
     6481 acatcctcgc gccgccccga tcgctggccg acatgacgcc cggagaacgg tggcgcgcgc
     6541 gaggagcgct gttcgccctg ggcttgacca tgaacgcggc ggtggcggcg ggcgtcctgc
     6601 tgggacccgt cctgggcggt ctcctcggct tggccgccgc cggcctcctg catctcggat
     6661 acaacctccg cttcgcgccg cggtggtctc tccccctggc catcttcgga cgaaaaacgg
     6721 tctccccaga ccgaccaaaa gaggcaacgc cgcccgcaac gatatcgacc gtcgagaccg
     6781 cccggctcct ggccgaactc ctcaacggca tcgagcccga ccagctgatc gagtgggccg
     6841 cggccgacgc ccgaaccggc cgagatttcg atcgtctcgc aaaagacatc gccgtccgcg
     6901 tgttcgaaaa aggcccgcgc ggggtctctc tcggacaagt cctcgccgcc ctggccgtcc
     6961 tccacgcgcg ggagggtcgc cccatcggcc acgtcgcggc ggcgttggac ggaaacgaga
     7021 aagacgccgg ccttttcttc gccctcttga cggataaaaa accggaagaa tccgcccatt
     7081 tccttcgatt cctcggtaaa accgccgagg ttttgccgcc gcaggaccgt ccggccctgg
     7141 aaaaattgat ggaggaattc atctcggccc aactgaaaaa atcgggcgac atgctgttgg
     7201 atttcgtccg gcaaaccctg gacgaagccc tcgccgcgca acgccaaacc ggggaaccgg
     7261 cggtggtggc gttccttttc accggcgccg aaccgctcta tcgggtcgcc caggcgataa
     7321 aatcgtccga tccgaaatac gtctcgctct ccctgaagcc gctgtatctc accaaaaaga
     7381 tcatcaacgg ccacgagaag ccggaggtct tccgctacct gcaggcacag ggactgttcg
     7441 attgccgcac gctcattctg gcggacaccg ggttcctcgg cagtatcccg cgctacgtgc
     7501 accgcttggt gcgtgaaaac ggtctccgcg cccctcccga gatcaaagga cggatgctct
     7561 gctacaacac cgacgaggag gacaaccggc acctcgccca ggaccaaaga gcgggcttcg
     7621 acatccaggg atacaacacc cgctcccgga aattcccccg ccggaccgaa cttgaaaatt
     7681 ttgtctggtt cctcgacatg gcgctggccc acacgcacaa ctccccggaa aagattgcga
     7741 aggaccgccc cgtcctgtcg gaaatgcctc tgcccgttct aaggaacctg ggaaaccgcc
     7801 tcctcgccca attcctcggt tcccaacggt cgcccctcga tccagaggtc gtgtacgagc
     7861 cgccgacctt cctcaccgat cccggccgcc tcctgatcga cggagaaaaa gcccgctggc
     7921 tggcctccgg catggaaaac aacatcttcc agcatcccac gcagccctgg gtggtgcggg
     7981 tccgacgcct cagcggggcc gacgacgtga aactggaggc ggccaatttc gccctgctcg
     8041 ccaaaaaggg tctggccccc gctcccatcg ccaggggcgt caccaacggc cacggctacc
     8101 tggtggtcga aaaaatcgaa gggctctccc tcgatcgaat ccgtcggacg tccccctcgg
     8161 ccctggagcc ggggctctcg gcaacgcggc aacttctgac cgaactcctc cgggcgagac
     8221 tcttcctgca ggactggaaa ccggaaaacg tcatgttcgg ccatcggccc ggcgaaccct
     8281 cccgggccct ggtggtggac gcccgttatt tggaaaaaac cccgcaactt cccatccggg
     8341 aattggctct ccgttaccgg caagcgcttt acggtttcga cggctggaaa acgtgcgacc
     8401 cccaaaagaa actgctggcc ttcctcaacg acgtcatcga aggtcacacc atcctggagg
     8461 gagaagaaga gattttccga cgaggcgcgg tcgcgcacga catttcatcc aaaacaaccc
     8521 ccgtgccgaa cgaaccgtct gtcgtcctgg tcggggccga caactcccgc gatttcgacg
     8581 cggaagccct gacgcgcttg gaatccgccc gccggaactc cgccgaggct ttttcggaaa
     8641 aactcctggc cgaacagtta gcgcggctgg aaaagaacct ctccaaggtg aacggtttct
     8701 cattccgatg ggcgcaagcc atcgcggaac gtcttcgagc cgagcacagc atcaacgtgg
     8761 accttgcgcc gacccatttc acgctggcct ccgtcatcca ggaattgatc aagaacgctt
     8821 ttgttcatgg caaccggctg gatctctccc gctccgtgcg gatcaactgg gccgtagaaa
     8881 acggccaact gtccgtgacg gtcacagacc acggcacggc ccctgtcgag ttcacgaccc
     8941 cgccccgcgc acttgatgcg gaattcgaag cccgtctcaa aatcgcctcc gttgccggga
     9001 ccggcgaaat caccagcaac cacctgggcg ccaaactgat gcgaaaaaat gggtatgacc
     9061 tccgcgcaat gccgtttgaa aacgcggaag gccgacaaat cggcaattct gttgtggcca
     9121 cgattccgct gactcaactc tccggccccg tcgaggatcc tgtcctggcc cgctggcagg
     9181 ctcgtcgctc acaagaaacg agcagccgat ccgaatcctc cggcaaatcg atcattgacg
     9241 cgacgcccca cggcctgttc cccttctggc cgcggacgtg ggtgcggaaa tatcccaaaa
     9301 cgctcgccac cgtcgagacc gtgatcttgg gcggactggt gggaggcctg tcggccctcc
     9361 tcacccatta caccggttgg gatctgaaat gggttctctc gggcgtgggc gccgcaaccg
     9421 cccttttctt cgcccttctg cacggagcgg tttactacga catccacgcg cctccccgct
     9481 cgctggccga catgacgccc agcgagcggt ggcgcgcgcg agggacgctg ttcggcctgg
     9541 gattggccat gaacgccgcg atggcggcgg gcctcctgat gggtcccgtg gtgggccccc
     9601 tcctcggctt ggccgcggcg atccttctcc acatcggcta caaccagtgg ctcgcccccc
     9661 gcgcgggact tcccctcggg acgatcggcg gtgaaagctg gcgtgacgac tacgaagccc
     9721 ccctgagccg gagctttgaa tggttcatca accaggcgaa ggaactcgtc ctgccggcca
     9781 agacgcggga aaaattccac gagaccggca aagcgaaaga tttcccggga ctctccgccg
     9841 tcctgacgtt caagaagggc tcgccgacgc acgatcgcgt aaggacgttg atgggccaat
     9901 tgaaagggga tttggagcgg gccggcgtct cccattactt tgcgttcctg gatgccgcgg
     9961 actacgagcc ccacatgacg ctctacgaca ccgccctccg gccggaccgc ccgctgacgg
    10021 acgaattgac ccgcaaggtc aaggacgcct tcacgggact ggcgcaggaa aacctcgtcc
    10081 ccgcggaatg gtcggccgcc ggctgcgcca tctccgccgg agaaggcggg gcctccatcg
    10141 tcctgcggac ccacccgaaa caaaaacagg acctggacac cgccctccga gtccgggacc
    10201 gcctgcacgc cgccctccga tccccggaca gaggcgtcgg aacggacgtc ccgttcatcg
    10261 gccacacgac actggcctac atcgtcaagg acatccctcc cgccgattat cgaaaagtgc
    10321 aggacatcct ccgccgctac gtgttccagt ccgccggcaa cttccttttc gaccgggtcg
    10381 agttcaggaa attcaacacc atgttggact gggacgaccc cctggccgaa ttgacattgc
    10441 aaccgggcag cgccgtcccg cagaagccga agaagaaatg gctgatccac gacaccgggg
    10501 ctgcccccat cgcggagatc gccgggtttc tgtccgtgac cggatatccc cagctcatcg
    10561 ccagggaact cacggaagcc atgaaccggc ggctcgccga agggaagaaa gtcgccctgc
    10621 tttcgccgga ggtcgggcgc ctgctcatgg agtacggcct gtggacggga gacgccgcca
    10681 tgaatttctt cggaaggttc gaaaaggcgg acaaagcggt cattttcacg gcgctcaaaa
    10741 aagccgatat cccggtgctc aaacgctatt tccaaaagga ccacccgccg aagccggacc
    10801 tgtcgcgatc cattttcgaa gtcctggcca aaatattggc gcggcgcaaa aacgacaccg
    10861 ttgaaaaggt cgcccggttc ctgaacgccg acggccggca gaaggagtcg gattacaacg
    10921 acctgccgca catgcgggaa gccgtcaaac tcatcaagga cgccgtcaag aaacaggaga
    10981 agatcgtcat cttcggcgac tacgacgtgg acggcgtcac cgccaccgcc atcatgctca
    11041 agaccctcca ccggattctg aaagccgagg ggatcccgcc ggaagactac ggaaaatatc
    11101 tcggctatca catccccaac cgcttcaccg agggctacgg catgtccgtc gcgggggtcg
    11161 acgaggtcat tgccgaaggg gcgaagctcc tcatcaccgt ggacaacggc atccgctgcc
    11221 gcaaacccgt ggaacgcgcc ctcgaactgg ggattcgcgt catcgtgacc gaccaccacc
    11281 agcccgacaa agaaaacgcg gacacccccg tcaacgtccc tctcatccac gccatgtaca
    11341 tcgaagaacg ggatcatccg gccaaacaca tctccggcgc cggcgtcgcc tacaaactgg
    11401 cccaggcgct catggacgat tacggcatcc aacgaccggc cgacgacctc ctcgacctgt
    11461 tcgccttcgg cgcggtggcg gacctggttc ccctgcgcgg agacaaccgg ctcctggtga
    11521 cacgggggct tgaactcatc aacagcccgc gcgtgagtct cgggatccga aagcttctgg
    11581 aagtcgctcg gaagaacggg aaaatcaagg aagaccaact cgtggaaaca ggcaccatcg
    11641 gctactacct ggcccccatg atcaacgcct ccggtcgaat cgcctccgcc acggaagccc
    11701 tcgaccttct gatgaccgag gacgcggcga tggccgacga atatgccggc cgactgtatg
    11761 aactgaacat ggaacggcgc gagatcgagg ctcagatggt ggaacttgtc gaaacgcggc
    11821 tgaaaaagga atttgactcg caaaccaagg gggccatcgt ggtcggcgag gaaggatgga
    11881 acgaaggcgt catcggcatc ctggcctccc gaatcgtcga aacgtgggga ttgccggcgg
    11941 cggtgttttc catcgacagg gaaaagggca tcgccaaggg ctccatgcgc accatccccg
    12001 gcgtcagcgt catcgacgcg ctgacggaat gccagaagca ggggctcgtg ctcaagggcg
    12061 gcgggcacga ggccgccggc gggtgttcgg tcgcgctcga caagttcgag gaattcaaac
    12121 gcgcctacag cgacgccgtc cggcgccaga tggagacgaa gaacatcgaa ccgttcatcg
    12181 aactggacgc gctcctggcc accgaagata tgggaatccc gctcatccaa ttgctggaaa
    12241 aactggcgcc gttcggggtc ggcaacgccc ggcccgtctt tgccggccgc ggactgcggg
    12301 taacttccta caaaatcagc aaggacggga aacacgttcg cggaatccgc ctggattccc
    12361 ccaaccgttc gcttgaagcc gtggcctggc ggatggccac gccggaaatc ctccaactgc
    12421 tcaacgactc ggtccgcacc ggaaaaaaac tcgatcttat ttttaccctg gagacgagcg
    12481 acttcggcgg cgtgcacccc cagcttgtcc tgcgggacat ccggccggcc tccgagacct
    12541 ctccggccga aaccgcccgc ctcgcgaaag ccgtcgtcgc cggcggcccc ctccgccccg
    12601 tcacacctcc ccgcgaacga ctggaaaaaa tattgacccg cgcgggcaac ggggacctcc
    12661 cctctgacga catcgacgac atcctccagg cttgggaagg actgcgggtc tacaaataca
    12721 agaccctgga aaaagagagt gtcgactttt tcatccggct cctggaacgt ctcaagaaat
    12781 tgccggacgt tcagcgtgaa gaaattctcg accggcttca tccgcttttt gaaacaatgt
    12841 tggacgatca atctgaggat tggcggcagt cgttcctcaa cctccggtcc ccgaaagatc
    12901 aaccccggcc cctctgggcc caacctctgg acttcaacgc attgttcgac gacacggacg
    12961 tgcttctgtt cggcgacacg cacacaatga tctcccaacg ggcgttcctg gcgcagcgca
    13021 tgcgggagct gaaagaagcc ggtgtcacgc atttcgttta cgaaggcgtg gggaggcagg
    13081 tcaacaaccg ggtgagggaa gacagacggg tcacgctgat aaaaggcgac aacatcgcca
    13141 cacaaatgct gcgccgggct gcggaagaag ccggaataca gttcgttccc atcgacatgt
    13201 cggcccaact gatggattcc atactgcgga agttgagttc ctttgcaagt gcgcgtttgg
    13261 gcaggtctta cgactttgaa actctgaaac agtatcttac aacagacacc gtctacgacg
    13321 aattggtcat gagacggcga aacatttatt tctcgaactt catcaaaacg atcctgcgga
    13381 acaacccgaa cgccaaaatc gccggaatca tgggtgaaac tcaccttacg tccaaacgcg
    13441 tccgaggcgg cgccacgggc gtcgttgaac gacttcgttc cgccggcatc catgcgaaag
    13501 gcgtgcggct cgaaaacgcc gaatccgccg aggcccccct cttcgacggg acacgatggg
    13561 acgcgcccgg tcggggatcc accgtctaca tccctcgtga catgacctac tggggacgtt
    13621 atatcctcga tacctttctt accccgccgg cgacccctcc cgaaaaaacg cccgcccgca
    13681 aagacggcgg gtcgctggcc gatcgaatct ctcgggcgtc ctggcgaagg gtctcggaac
    13741 cctccctggt cggccacgag gtggaggttt tcgtatcgga tgaactgggt ctcgtggtca
    13801 aagtccctcg tccaaaaaac aaaaagaaat ggaacaccgt ggtcaaacca cacgctcaaa
    13861 ccgccattga gaggctgggc cccgaggcgg cgaacgccgc gcctctcgcc cccccgaccg
    13921 ttcttctgaa aaacatccaa ttgactgtgg acgggcaaac cctccgcttt gacgaaggca
    13981 tcgcacaggc gcgcgttccg ttgctgggcg aggtcatgca cgaactggac aagaccggcg
    14041 acctggccgg cacgaccgcc cttttgaacc agctgatcgg actcctgacc gacctggccc
    14101 gacgcggcct catttataac gacgagttcg tccttaagaa tttcggcgtg tggcgcggca
    14161 aggacggccg gctgtctgtc gtggtcatcg atttcggagc gctcacgccc ggccagggac
    14221 tcctggacaa agacccggac ctcatctatt tctaccccgc ctacgtcgat tccctcaaag
    14281 ataacctgaa gggctggcac accccctacg gaaaattccg ctatgcggaa aaactcatcg
    14341 cgtgccttga cgcggaaaaa tattttctga cactgaacca tctactgggc gaactgggag
    14401 gattggccaa accgctggcg ccgccgacgc cgagctctcc tcaaaaagcg gcccggccgt
    14461 tcgacgtctc gcacaacgca acggcgtggc tggcgggcgc ggtggccctc tccctgggac
    14521 tcctcctcac cgttttcggc gccgcgttcg tggcccacac cgccgacccc gccgcctttt
    14581 gggtcagtct cctcgtcctc ggctggggcc agcttttcac ctggaacggc caggcctatc
    14641 tgcgacgcta ttatgcggac tggaaaaaag ccaactcgtt ccgggccggc accacaacac
    14701 ggccggcccc ctcggaagaa accaccccca tcgacgaaga gctctggcag tcctacgtgg
    14761 cgggactaaa gaagatgaat ccggacttcc gcgccgaaga tctccgggtg gactgggacg
    14821 gccaaaaaac ccgcggctcc cgtctggcct ccaccgacga ggatgggaat gtctggctgg
    14881 acccgtggct ggtgacggac gttcgccaat gggtcacggc ccaaaaccgg acggctctgg
    14941 aacagtcgga cgtgcgcctc ctcttgctcc aaaaggaact gctgcccctc atcgttcaac
    15001 acgaaggact gcgcgtccgc tacacgcagc atcccctcct tcaaatccca cttctgcaaa
    15061 ccctctggat ctatctcacc aaacccgcgc ccgaacccat tgacgttgcg gcggcgtcgg
    15121 cgaaaaaaga accgacacgt ctcgaacggg tccttcaaga catccggttg ttccggaaaa
    15181 attttacgcg ccttgaatcc ctccttgccg ccgatacgca aggggatcct cttcatatcc
    15241 acgggcacgt cgcgaacggc ctggccctcc tgtcatcgct ggacggggaa cccgtaaccc
    15301 ggccggtgga agaatgggag gacctgcgtg accgcctggc gcggacactg aacgacattg
    15361 agaggcgcgt cggagcctcg atgtttcccc gcagcggacg gccggagtcg gaactgcaac
    15421 aaaaactcga gtggctcaaa aacgacgcgg gcatcacgtc ggaaaccacg gccgagtccc
    15481 ggcggacata cgaacgatat ttatcgaacc ggcaatcgga tcccctccaa ttgggaatca
    15541 ccggcggcct ttacggcggc aacacgcgtc ttctgctggc gcgccggctc ggtctggaac
    15601 cttaccgaat tcccgtaaca acggtcgtga gaggcggcga acaaccggag acccttctga
    15661 cggccgcggt ggcgggccgg ctggacgccc atcgacggga aagccaaatc accgacgccg
    15721 accgggccgc ccggctgggc gccctcgttt cgcgacttct gcgaaacctg gttgaatccg
    15781 gatatatgtc cgccgcggac agccgggatt tcgtcaccgc ggaaaagtcc gagagctggc
    15841 ggcggagcct ggcggccgac aacctggatc cggtcaaaca cgccgtcttc atcgaaggcg
    15901 cgctctggca gagactctac ggagcggttc gggaccgcgc cccgggagtc tctctctcct
    15961 cggacgaatt gaccgctgcg ctcgataccg cgatgatgcg gttgaccgcc aaagacgaga
    16021 aaaaagccgc gacgacggcg gcaaaacccg ttttgcccac gacaccggaa aaaccggccg
    16081 ccccctctga acggacggtc gaaacgcctt ctttcgatct ccgctccggc gggttccccg
    16141 cccttctcca cggactcggc gtgtggacgc ggctggcccg cgcgacccgg ctcgtctatg
    16201 cgggcctgac gctcggcgtt tcccccgtcc aactggcccg cgccgaatcc atggcctatg
    16261 ccctggcgga aaccgccgca aaagccatgt tgaccgcgaa aaccggcatc aagacagaac
    16321 aaaaagcgga cggcactctg gtcaccgaaa tggataaacg catccaggac ctggccgcgg
    16381 ccgagatccg tcgccgcttc ccccgacaca cgatcttcgc cgaagaaggg gcggcccagc
    16441 ggaccgtgag cgaatggatg tggacgattg atcccatcaa cggcaccaaa gcctacgccg
    16501 aaggcggcac ggaatatact ttcgacatgt cgttgttgca caacggccgg ccggtcttgg
    16561 cggtcagttt cgcgccggaa atgacggcgg cgggccaacc gggagtgcgc gccatcggca
    16621 gccgatggac cggcactcta ttgaacggaa aaccggtccg ttttgatcgc tcccaagatg
    16681 gaaaacggga ctgggacgtg atctcccgct cgagcgtctc ccgaaaaaaa gacgatgcgt
    16741 atctgcggac ctggcgggac ctctatgggg aatcccacgt ccggcaagtc agcaacggag
    16801 cactggccgg cctgcaaatc ctgaccgaac agaacgacgc cgtgttctcc aacgtggtgg
    16861 cggaaccctg ggacccggtg gccatctccg ctctcctgga atgggccggg ggagcacccc
    16921 tccgatacga cggcgcctcc tacttcccgc tcactccggc cgatttcgac cgcgccgggc
    16981 agggatcttc cggttacatt ctgacccgcg gcgggatcca cggcaagcgc gccgcggaag
    17041 cggcctctct ctccaacggc cttccggaac ccgcgaacac gccgatcgac atcggcgtcg
    17101 acattcaact cgccgtgccg cccgttcaac ggccgctttc cgaaattaaa gattccgatg
    17161 ttttggtcgc ccgcctccta cctttggcgg gcgattccag cgcctccagt tcttccccct
    17221 ccctcctgga ggcgctgact ctttcctccg aaaaaacggc ggccggaacc gggaaccggc
    17281 tgccgctgga acgactgtct cccgaggcgg cccgacggct ccttccttat atgacggatg
    17341 cggaacgacg cgcctacggc gcgaccctcc ggcgagaggc gaaaaacatc cgggattccc
    17401 tgtcggccgg ccgcgccgac gaaggcgcgt tccgggaact cctgcggaac gtcggacgag
    17461 tccacggaca gggcggcgtc ctgttcatgc cggccaacgc cgcggacatc ccccgacaaa
    17521 aacgccgcga aatgggccat ctcgtccgtc tcgcttatga gtccggactg attcccgccg
    17581 gcgccgtgga cggcgtcgcg gcccaaaaac ttcagggata catggacgtc tataaccgcg
    17641 cccggcatac cgccgaaatg cggacaaacc ggtccgccgt cgaccggctg agggaatcgg
    17701 cctcctctct cctccggaaa ctccgcgaac tcgtcctggg agacgcctcc ctgcgggtgt
    17761 tccacattcc cgtgtcgcgc gatgcggccg gaaaactcct cctgctggac gacgcgcgca
    17821 ccggactctg gctggaccgc ctctcccgca ccctatcgac ggaaggacaa aaagtcagga
    17881 ccgtcctggt gacgaacgaa tcactttcgt caaatgaaaa aggcgaactt tgggacctgg
    17941 tcgtcgcggc gctcgcccgg cgaacggcct ccgccgcgga ggcggcgcag atcgccgccc
    18001 ggttgaaagc ttctctggcc acggaccgac cgatcctctt ccgggaagac gtcggtgacg
    18061 aaacccgccc aggcatcatc caggcccggt ccctttccaa atacctgaaa ggcgtcccgg
    18121 ccggggcggc catggacatc tacacgctgt gggaggatca gtgggacctc tccggcctgc
    18181 gcaaagaagc cgtccggctg ctcgtccttt tttccggcga catcctcttc aacgccgccg
    18241 agtccctgga gaaatccatc gccaccatcc gctacatcca gatccaggcc tgaccttccg
    18301 ccttccggcg tcacgccgcc ggaccgcaaa aaattcgtat aatacgcgca tcttgaacag
    18361 gatctatttc cccgaagaaa gaatcccgct cgtcaaactc ctccccatgg ggatgcagca
    18421 cgtggtagcc atgttcggcg ccacggtgct cgcgcccatc ctgatgggat tcgatccgca
    18481 ggtcgcgctc ttcttttccg gcgtgggaac cctcctcttc ctcctcatca cccgtttcaa
    18541 ggtcccgagc taccttggct ccagtttcgc cttcatcggt ccggtgctgg ccgtgacctc
    18601 gggccggccg gagaacatcc cgttcgccct cttcggcatt gcgggagccg cggtcctcta
    18661 cgccgccgcc gcggggatca ccctccggtg ggggtccagc tggatcgacc gcctcatgcc
    18721 gccgatcgtc accggcacgg tggtctccat catcgggttg aacctcgcct cctcctcggt
    18781 ggcgaacgcc ctcaacgccg attttgccat ccgcaccggc gccgatgcga tacggctgac
    18841 ggtggccgcc gccacgttcc tcaccgcggc cttcgtctcc atctacatga aggggttcct
    18901 gcggctcctt cccatcctca ccggcgtggt cgtcggctac atcgtcgcgg ccgtcgcggg
    18961 gatactcgac cccgcggcgc tgcaaaacgt ccgcgaggcg gcgtggatcg gcctgccgcc
    19021 gtttcaaaca ccccgcttca gcgtgcaggc cctgctggtg atcgcgcccg tcttcgtggt
    19081 gctcgtggcc gagaacaagg ggcacatcgc ggccatctcc gggtacatgg gccgggacct
    19141 caacccccat ctgggacggg cctatctggg cgatgccgtg gccagtttcg tctccgccct
    19201 gggcggcggg acgccgcaga ccacctacgc ggagaacatg ggcgtgatgg ccatcacgcg
    19261 cgttttcagc acctacaact tcctgatggc ggcggggatc gccgtcctcc tcggcctctg
    19321 tccgaaattc ggggccgtca tccagtccat cccggccccc gtcctgggcg gcgtcaccct
    19381 catcctctac ggcctcatcg ccctcatggg cgtgaagata tggaccgacg ccaaggtgga
    19441 tttctgcgac catcggaacc tggtggtggc gggttcctcc ctcatcgtgg ccacgggatt
    19501 gggcgtccgc ggactcaccg tgggcggcgt caacgtggcc ggcatcgcct tcggcaccct
    19561 tctggccctg gcggcgaatt tcctcctcaa cctccagaaa ccgtcccctc gcgccgccga
    19621 agacgtgccg gacgccacca gcatctgagc ttttgccgtc tctggacaaa acccgaaaaa
    19681 tatcacatca tcttttcaag aaagagaggt gattcacaca tgaaaatcag aatgatcgcg
    19741 ttgcttgctc tggcgagcct ttctctcgtg gcgggctttg gttgcaaaaa agaagctccc
    19801 atggaagaac agccgatgca ggagcaggcg gccgctcccg aagcggcggc tcccgaagcc
    19861 gctcccgaag cgccggcgga agctccgcaa ggcaactaag tcttcattca tctgttctac
    19921 tgttctacaa aatccgtcgg tcctcccctc cttgggggcc cggcggattt ttttatgccc
    19981 cgttttttca cgactcgact tctcccgtcg aaatgataaa atagcggccg cttatgccac
    20041 gtttcccctc aaaaaccctc ccgcctcccc tttcgagaaa tccgctcgac cgccgagacg
    20101 ccgcgccgcc ttctccgagg aggcccgatg ctctttaact ccttcgaatt cctgatattt
    20161 ttccccgccg tcacccttct ctatttcgcg ctcccgcacc ggttccgatg ggcgctcctc
    20221 ctggcggcga gctgcctctt ctacatggcc ttcatccctc agtacatcct catccttttc
    20281 ctgacgatcg ccgtcgacta cacggcgggc ctctacatcg agaaaagcga agggcaccgg
    20341 cggaaaatgt tcctggccgt cagcctcgtg tgcaccgccg ccatcctttt cgtcttcaag
    20401 tacttcaatt ttttcaactc caacttcgcc cgcatcgcgg aattcttcca ctggaactac
    20461 cccatccagg ggctctccat catcctgccc atcggccttt cgttccacac ctttcaaagc
    20521 atgagctacg tcatcgaggt gtaccgcgga cgccagcggg cggagaagaa cttcggcatc
    20581 ttcgcgctct acgtgatgtt ctatccccaa ctcgtcgccg ggcccatcga acggccgcag
    20641 aacctgctcc accaattcta cgaggagcac cgtttcgact accggcgcgt caccgacggc
    20701 ctcaaactga tggcctgggg cctgttcaag aaggtcgtca tcgccgatcg cctcgccgtg
    20761 ttcgtgaacc acgtctatgc cgacccggcc tcgttcccgg ggatctccct cctcctggcg
    20821 accgtcttct tcgccttcca gatctactgc gacttttccg gatactccga catcgccatc
    20881 ggctcggcgc aggtgatggg gttccgcctc atggacaatt tcaaccgtcc ttatttctcc
    20941 aagtcggtcg ccgagttctg gaaacgctgg cacatctccc tctcttcctg gttccgcgat
    21001 tatctctata tcccgctcgg cggcagccgc gtctccgttc cccgttggta tctgaacctc
    21061 ttcctcacgt tcctcatcag cgggctctgg cacggcgcca actggaccta cgtcttctgg
    21121 ggcgcgctca acggggccta cctcgtctcc gagatcgtca ccgccgggcc gcgccggcgt
    21181 ttcgtgcagg ccgtcggact ggacaggctc ccccgtctcc acaaaatcgt tcaggtctcc
    21241 atcaccttct ccctcatctg tttttcgtgg attttcttcc gcgccgaaaa cctgagggag
    21301 gccttctaca tcgtcggaca catcttctcc ggcgtcggac ccttcgtcaa gaacttcacc
    21361 ctctatctgc tgaacgtcaa cttcaaactg ggcctgacgg tggacagcgc cgtccgcgac
    21421 cagctcctca tgggacagac catgcccgag ttccggcgcg ccctgctggc catcgggttc
    21481 atggaaatcg tccacctcat tcagcggcac aagaacatcc ggcacttcct ttcccaaaaa
    21541 ccgttcctcg cccgctggac cgtctattat ctcctggtca tcagcattct cctctgggga
    21601 cttttcaaag atgaaaaatt catctacttc cagttctgac gtccgccgcc cccttctcct
    21661 cttcgcggcc aagctggcgt tgatggcggc gctcgcggcg gccacctgcc tggccacctt
    21721 cgtgctgccg ctgccctacg accaaaacct ggccgtcatc atcaacaaac gggaccggct
    21781 caggaacatg ccgggagagc ggctcatctt cgtcggcggg agcggactct tcggcgggct
    21841 ggacgtccgc cgcctcgagg cggagctgaa gaaacccgtg gccaacctgg gctttttcgc
    21901 ggggttcggg atcttccatc tcctggacga agtaacgcct ttcctccgtc cgggagacac
    21961 cgtcctcatc gtccccgagt acggcctcat ccgccaggat ttccaaaaca acgaacaatc
    22021 gcggaagtgg atcctccggc tctcgcccgg gcgccatgcc cttcaactct acccgccgtc
    22081 aaaggacggg ctggtcaatt tcgttcagga ttccggcgca ctgcttctga gccaactggc
    22141 caccctccgc aaagccgtgg aaaccctctt caaacgggat acccgtttct acaccccgcg
    22201 gggattcgtc aaataccggc tcatcacgga cgaaacgggc gaaccgaccg aacatcaccg
    22261 cttcgccgtg gtcccggcgg accaagtggc ggggaaaggg gatacttatc cggacctcac
    22321 cgtaaaaccc gaatcgatgg ccctcctgaa caatttcggg gaggcggcgg cgcgccgagg
    22381 ggcccgcgtc ctcttcgtct ttgcggcgtt tccacgcgag gaatacgaac tcaacaaagc
    22441 ggccatcgac cggctccacc ggcaactgaa aaacgaactg cgtttcgaca tcgtcggcca
    22501 acccgaggat ttcatctatc ccggccccct tttcgccaac tccatcgacc acctcaacgc
    22561 ggacggcgtg cgcgaacgaa ccgacaagct cctcgcgctg ctccggcccc accttcaaaa
    22621 caaataggac gccggacccc gtcggaggaa taaaaaaaag accgtgacga cgtcacggtc
    22681 ttcctcccgc cggccaatcc gacggcgctt atcgaaggcg gcgcaaacgg ccgtcgagtt
    22741 ccttatagtg gaacaacgtg cgataatcca ccagggtcga caacgcccgg gaatggacac
    22801 ggatatcgtt ctccaccaag atggccaccg gattcagccc gccgatgacc accgcgcccg
    22861 cccgcccctc ccccaccggg atttccagga ggggttgccc cggccaaccg accgtcatga
    22921 acccgcccag ccccgcttcc tccagtcgtt tggccagttc gatcacgcgc tcgcggctgt
    22981 ccgccgggat ttcgcgaaag ctggctccga tgcgtccgct gccggtcttc accgcgccgg
    23041 tgtaatcggt catgccgctg cggatgaaaa tctccagcgg atccagcgtg gtcccctcgt
    23101 aatggatgag ttccgcaaac cggaccggtt ttctgtctcg cagctccaaa agaccgccga
    23161 agcgggaggt ggtggggatg ccgtgcgcca gaagaacgcc gttgatggtg atggaacaaa
    23221 cggtccccag ccccacatgc cccggcggaa ccacgatatc gccgatcttc tccccctgcg
    23281 ggaacagcgc cacgaggtgc cccatggcgt acccttcctc gaacacgcgg ctcaacaggg
    23341 gaatgttttc ctgcaagcgc ttggtttcga tgatgctgat gttgatgacg accccgcccg
    23401 tgcggttatc cagattgaag ttcatccggt aggtcatctg gtcgatcttg gccgccagat
    23461 agcccacctt ctcgatcacc cgggcggtcg aaagttcgcg gctccctttc tccgtgatct
    23521 gccgcccgcg ttttccgaaa ctttttgtga gcccctcctc atccatggcc aacagatgga
    23581 agcgcacggt ccgctcgctg atgtcgtgtc ccatggccaa cagatgttcc gtgatggcgg
    23641 aactgctcag gggccggacg gactcgtgaa ggaccttgag tatggcgaga atttttttct
    23701 cggttttctc actcattgaa atttcatgat accaaataat tgccggaaaa cagtattttt
    23761 atggcaattt tgccgataat ccgctctttt ttgaataaat agattcataa tttcataaat
    23821 aataaaaatt tcttgcattt ttttcggtaa taaaatggcg gcattgccaa aagtatctgg
    23881 cattattgcc atataaattt taattaatgg atgaaattta ttgatatctt atccatatta
    23941 tcctataatt ggtatacgtt taccagatag atcccgccca tactgaccga aacatccgtt
    24001 gttcgcgttt gtcatggcat cgtcttccct ggtcgcgaca tcttgattga ggcaagtttc
    24061 tgccactaaa tttatgcgca tcatcgaaaa aaacacactg aacgacaccc aaggaatgcg
    24121 cctcacgcga atgcgtttcg aggcgcccgc catcgcccgc aaggcgttgc ccggccaatt
    24181 catcctcctc atggtccacg agaagggcga acgcatcccc ctcaccatcg tggaaaccga
    24241 cgccgccgcg gggaccctca ccctgatttt tcaggaggtc gggcacacca cgaaactcct
    24301 cgggaagaaa aacgtcggcg actccctcta ttccctcgtg ggcccgctgg gacatccttc
    24361 ggaaatcccg cggaacgagc gcgtcatcct ggtgggcggc ggcgtgggca tcgcggagat
    24421 cctgccggtg gcccgggcgg tcaaggccgc cgggaaccac gtcgtctccg tcctgggcgc
    24481 ccggaccaag gacctcctcc tcctgacccg cgaagtcggc gaaacctccg accggctcct
    24541 gatctgcacc gacgacggca gcgcgggcga aaaaggactg gtcacggacg ttttgaaaaa
    24601 acttctggcg gagccggaac ccacccaccg gattttctgc gtggggcccg tccccatgat
    24661 gagggccgtt tcggaaacga cccgcccgtc ggggatcaag accatcgtgt gcctgaacac
    24721 catcatgatg gacggcacgg gcatgtgcgg cggttgccgt ctcatcgagg gaggaaaagc
    24781 gaggttttgc tgcgtggacg gaccggattt cgacggccac gccgtggact tcgccgacct
    24841 ggtccaacgc caacgccgct tccgcgaaga ggaaatgaaa agcctggacc acgcctgccg
    24901 gctggaaggg accgccgccc catgagcacg ccgtcccgcc cccggcccga accgggccgc
    24961 atccacaaga tttccatgga cgaacggaag acctccttca ccgaggtctc ccgcgggttc
    25021 ccgccggagg aagccgtcaa ggaggcggcc cgctgcctgc agtgcaagaa cgccccctgc
    25081 tccaaaggct gcccggccgg catcgacgtg cgggaattcg tccgtttcat ccgcgaacag
    25141 aatttccagt cttccatcga caagatccgc gagagcaaca gcctccccgg cgtgtgcggc
    25201 cgcgtctgcc cccaggaaga gcaatgccag aaggcgtgcg tgttcaacgc caagaaaata
    25261 cccatccaga tcggatacct cgaacggttc gtggcggatt gggaattggc ccacgggaaa
    25321 agacaacccg cgcgttccgc cgcccccaaa gaaaaagtgg ccgtcatcgg cgcggggccg
    25381 gcgggactga cctgcgcggc cgatttggtc cgggaagggt tcgacgtcca cctcttcgaa
    25441 gcgctccacg tcgccggcgg cgtcctgatg tacggcatcc cggaattccg tctgcccaag
    25501 gacatcgtga aaaaagaggc cgactacatc ggcagcatgg gcgtcaagat cgagacggac
    25561 gccgtggtcg gccgcaccta caccatcgac gaactccgcc gggacggcta caaggccttc
    25621 ttcatcggcg tcggcgccgg actgcccatc cttttgaaca tcccgggcga aaacgccaac
    25681 ggggtgtact cggccaacga attcctcacg cgggtgaacc tgatgaaggg ataccgtttt
    25741 ccggaatacg acacgccggt gcgcgtggga aaacgggtgg gcgtgttggg cggcggcaac
    25801 gtggccttgg actgcgcccg cagcgccctg cgcctgggcg ccgagagcgt gacgctcctc
    25861 taccggcgca cccgcgacga gatgcccgcc cgccaggaag agatcgagaa caccgaggag
    25921 gaaggcatcc acctgcagtt cctcgtcacg ccccgggaga tcctttccga cgacaaaggg
    25981 aacgtccgcg gcctgcggtt gttgaaaaac gtcctgggag aacccgacgc ctccggacga
    26041 cgttcccccg tgccgcagcc cggcaccgat ttcgaactgc cgctggacac ggttatcgtg
    26101 gccgtgggga ccgcggtgaa ccccctcctg ctgtccacga tggaaggcct ggaacggacg
    26161 aagaagggct acatcaaggt cggggaggac atgcgcacct cgctgccgga cgtgttcgcg
    26221 ggcggagaca tcgtcagcgg ttccgccacg gtcatcctgg ccatccaaca ggcccgcacc
    26281 gccgcccggg ccatcaccca ctatttacgc aacgggaaat aacgttgccc gacggccgaa
    26341 aaggcccgtc acattctcat taaaggggag cctcatgcat acagcgttca tgaaaatggt
    26401 cgtcgaaaaa aatccggggg aaacggaatt ccaccaggcg gttgaggagg tcgtcacctc
    26461 cctcctgccg ttcatcgaaa aaaaccccca atacaaacac gccaagattc tggagcggat
    26521 cgtggagccc gaacgggtcc tgatgttccg cgttccgtgg gtcaacgaaa agggcgaggt
    26581 ccaggtgaac cggggcttcc gagtgcagat gaacagcgcc atcggcccct ataaaggcgg
    26641 attgcggttc catcccagcg tgaacctggg catcctcaaa ttcctggcgt tcgaacaggt
    26701 cttcaaaaac agcctgacca ccctccccat gggcggcggc aaaggcggtt cggatttcga
    26761 tcccaaaggg aagtccgaca cggacgtcat gtggttctgc caaagcttca tgaccgaact
    26821 ctgcaaacac atcggaccgg acaccgacgt ccccgccgga gacatcgggg tcggcggacg
    26881 ggaagtgggt tacctcttcg gccaatacaa acgcatgcgc ggcgagttca ccggggtgct
    26941 gacgggcaag gggctgggct ggggcggcag cttgatccgt ccggaagcca ccggctacgg
    27001 ctgcgtctac ttcgcccagg aaatgcttaa gactctccac aaagccgtcg gcgggcatgt
    27061 ctgcgcggtc tccggttccg gaaacgtggc ccagtacacc gtggaaaaac taaacgaact
    27121 gggcgccaag gtggtcaccc tctccgactc ctccggattc atccacgatc cggacggcgt
    27181 cgacgagaaa aagctggcgt tcgtgatgga attgaagaac ctccggcgcg gacgcatcaa
    27241 ggagtatgcc gataagttca aatgcaagtt ttacgaaggg aagcggccct ggggcgtcaa
    27301 atgcgacatg gccttcccct gcgccacgca gaacgagatc gacaaaaccg acgcggagac
    27361 gcttcttaaa aacggctgcc tctgcgtggc ggaaggcgcc aacatgccca gcaccctgga
    27421 cgccgtcgcg gcgttcgtga aggcgaagat cctctatgcc cccggcaaag cggccaacgc
    27481 cggcggcgtg gccaccagcg gcctggaaat gtcccagaac agtctgcggc tcagctggac
    27541 gcgccaggaa gtggacaaga aactccacga catcatgatc aacatccacg agtcctgcgt
    27601 gaaatacggg caggaaggaa aatacgtgaa ctacgtcaaa ggagccaaca tcgcggggtt
    27661 cgtgaaggtc gccgacgcca tgctggcgca gggcgtggtc taaccggccc gccggaacgg
    27721 aaaagatttt tcttcgttcg ttttaagaaa aatcttcgcc ctttacgggg ccgttaaaaa
    27781 cgaagagcgt tcgtttgcat acccctgtgg tatgcaaacg aacgctcttt ttttcggaag
    27841 atcccgggcg cttcaaaaat aaccggaagg cgaaaccgat gttcgttaaa aaatgccggg
    27901 gcttcgatga cgaaaaacgc gggggctgac gttgagggcg gaaggccgcg ccggaccggc
    27961 gcgggtcagg cggagaggag actcttgatc ttctggatga gctcttcggg gttgacgggc
    28021 ttgctcatga acccgtcggc cccgacgttc tccgcctggc ggcggtccgc cgggaaagct
    28081 ttggcggaaa gcatgagcac cttgatcttt ttgagggcct cgttcgactt gacctgatga
    28141 cagatggaaa aaccgtggac ctccggcagc atgatgtcca gcaccaccag gtcgggcggg
    28201 gcgttggtga tcatgttcaa cgcgatctgg ccgtccgcgg cggtcgtcac ctcgaaacca
    28261 gcgctcatca cgatgtattt gaggagttcc cgggtgcttt cgtcgtcttc ggcgatgagt
    28321 atcttggcca taccggcgtg attataacgg tttcccctcc ggccgtcaac cacgccctcc
    28381 gatggaaaaa ccgggtcgaa tgtgatacca tggactccga ttttcacacg gaaccgacac
    28441 ccaatgatcg aacttcccag catctataat cccgaacaga ccgaatcgaa gcggtacgac
    28501 gtctggatga aacacggcct tttcgggtcc caaccggacc cgaagcggtc cccgttcacc
    28561 atcgtcatcc ccccgcccaa cgtcaccggc tccctgcaca tggggcacgc cctcaacaac
    28621 acccttcagg acgttctcat ccgctggcac aagatgaaag gggacaacac cctctggatc
    28681 cccggcaccg accacggcgg catcgccacg caaaacgtgg tggaaaagat gctcaagaag
    28741 gaaggcaaga cccgccacga cctcggccgg gaaaccttcc tcaagcgcat gtggcggtgg
    28801 cgccaggaca ccggcgacac catcctccat cagctccgca agatcggctg cgcctgcgac
    28861 tgggaccgca cccgcttcac catggacgag gcctcctcca aggccgtgat gaccgccttc
    28921 gtggaacttt accggcggca cctcatctac cgcggacggc ggctggtgaa ctggtgtccc
    28981 cgctgtttca ccgccctggc cgacgtggag ttggattacg tcgaatccgc cggacacctc
    29041 tggcacatcc gctacccctt cgccgacggc agcgggcgca ccctcgtggt ggccaccacc
    29101 cgcccggaga ccatgctggg ggacaccgcc gtggcggtgc atcccgacga caagcgttac
    29161 gccgacgccc gggggaaaac cctccgcctg ccgctgatgg accgggagat ccccatcgtt
    29221 ttcgacgccg cggtggatcc ggccttcggc accggcgcgg tgaaggtgac gccctcccac
    29281 gacgccgccg atttcgagat cgcccgccgg cagaacctgc cgcacgagat ggtggtgggt
    29341 tacgacggcc gcatgaccga ccgcgccggg aaatacgccg gcctttccct caaggaatgc
    29401 cgcgaaaaag tgctggccga cctgaccgcc ggcgatttcc tcgagaagga agaaccctac
    29461 aaacattccg tcgccacctg ctaccgctgc gccacggtca tcgaaccgct ggaatccagc
    29521 caatggttca tgaagaccgg cgacctggcc aaacgcgccg cccacgccac ggaggtggac
    29581 cgcgtccaga tttttccgga gtcctgggag aaaccttaca tccactggct caccaacaac
    29641 cgagactggt gcctctcgcg ccagatctgg tgggggcacc gcatcccggt gtggtactgc
    29701 ctccggtgca gccgcctgaa cgaggaccaa ctgggccaga tcgcccggga cccgacgttc
    29761 cagctcgaat ccaccaaaat gatcctgcgg gaatccgcct ccgaccccat cgtgcgggag
    29821 gaggaccccg tggtctgccc gcgctgcgga aaagaggaga tcatccagga ccccgatgtg
    29881 ctcgacacct ggttctcctc ggcgctgtgg cccatctcca cgctgggctg gccgaagaaa
    29941 acgcaggacc tgtcctacta ctaccccacc tccgtcctgg tgacgggtca tgagatactc
    30001 tatctctggg tggcgcgcat ggtgatgatg ggagaagaac tggccagcga ggtgccgttc
    30061 cgccacgtgt tcatccacgg catcgtgcgc gacaaacacg ggaagaagat gtccaaatcc
    30121 ctcaacaacg tgatcgatcc cctggacatc atgaagaagt tcggcaccga cgcgctccgt
    30181 ttcgcgctga tctcctcggc catccccggc cgggacatgc agttgtccga cgacagtttc
    30241 gtgggagcgc gcaacttcgc caacaagata tggaacgcct cccgcttcgt gctcacgaac
    30301 ctgtcggacc accgccggac cggcctccct cccgataaaa tggacctgtc cgaccgctgg
    30361 atccgccgac ggtgcgtgga gacgctgaag aacgtggacg ccttcgtgga agggtacgac
    30421 ctggcccagg cggcccgcac gctttactcc tttttctggg gcgagttctg cgattggtac
    30481 atcgaactgg ccaagatacg gctccaaggg ctcggggggc cggcggagaa cccgccgccg
    30541 aaaccgccga ccaccgacgc cgccctcgcc gccaaggaga cgctggccac ggtgctggat
    30601 tccgtcctgc gcgccctgca tcccatcatg ccgttcatca cggaagaact ctggggcgcg
    30661 ctcggcgaaa ccctcggcga gaaaacggaa ggctgcctga tggccgcgcc cggccgcgtc
    30721 ccggcgctgt tcgattcccc cgccgcctcg gaagccgaca tgcgcgccat ggaggtgctg
    30781 atggagaccg tcaccgccct ccgcaccgta cgctcggaaa tgaacgtgcc gccgggcaag
    30841 tcggtcccct tgctggtgaa cgcgcaaaac gcgctcccct tcaacaaggg cctcttggaa
    30901 acgtatgggg attacgtgaa gcggctggga aaggtctcgg agatacggtt catcggcaag
    30961 ggagagaaac cggcccaggc ggccgcggcg gtggcgtcgg acttcgaact tttcatccca
    31021 ctggaaggac tgatcgattt cgaaaaagag aaacagcgcc tctcgaaaga ggtcgtgtcg
    31081 ctggacgcgg acctgcagag actccaagcc cgcctgtcca acccggattt caccgcccgc
    31141 gcgccccagg aagaagtgac caaagcgaag gaccggcaca ccgaggtcac cgccaagaaa
    31201 cagcgcctac agacacacct cgccgccctg gcgaaagatt aattccccgg cgccgccggc
    31261 gcctttccaa cccgcaagat attatagtcc ccgaaaacgt ccacccgttc gaacgacccg
    31321 ccgtcctccg ccagttcctt ctccgccctc tcggaggaaa cgaccgccac ccccgcttcg
    31381 ccgcggccat cccagatccg aaccttcccc cactccgtct cggtggacgg gaggaggcgg
    31441 aaccgttccg gcattccgga gaacacaagc acgttgggcc agccgctgtc cgcgacttcc
    31501 aacaggatga gggattccgg acgaccgagt ttcccctcgg cattcaattc ccgaagcctt
    31561 ctccccagcc cccggttgtg gtcctccagg gaaagacggg cggggacctc tttcatggcc
    31621 cgctcccacg accaccccac ggggccggcc gcgagcaaca gccccagcaa catcccataa
    31681 cgtccgagag cggacgccgc ggcccacacc gccgcccccg ccggcgccat ggccaagaac
    31741 aataccgtgt tcatgttccg ctcggtctcc cacgaaaaga gatagaacca ggaacccgcc
    31801 cacaacagga aggcctgccc cagaaccaaa acggcgaacc agccgagcgg ccggaagaac
    31861 ctcttccctg aaaaatgcgc caacccggcg cacaggaaca cgttgaacgg gaacgattcc
    31921 ttcaacaacc acgccggcaa ccgcaggaaa cgctggccga acgacaacca ttggtcggcc
    31981 gcgttggccg ttgaggcgcc gcggccgtga accatcaacg acggcaatat cacatagaga
    32041 cagaccagcc cggcgccgac gaagaacggc caaaaacgcg gccggcgccg ccaacgcagg
    32101 aacagaaaga tccccgcgag accgaacaat ccgagaccgt cggaacggac ggaggccgcg
    32161 tagcccaaca ggcagaacgc cgccaggtcc gcccagaaat tggacggctc gtcgaggaag
    32221 agcccgtaac acgcgatcca aaacgacagg gccgtgaacg ccagggcggt catttcggtc
    32281 tgcccggaaa agaccagccg gccgaacgac ggcacgctca acgccaggac cgcggaaacc
    32341 gtcgccgcgc cgggaccggc cagccgccgc accgcgtcga agagcagcca caccgccatc
    32401 aatcccaacg cggcgttcac cgcccaggcg gcataaaaaa cgtccaaccc catccgcgtg
    32461 gcggcggcgt acaggaatcc ggccaggggc acgcgcggga aggaccactt cagcaccccg
    32521 gtggcgtgcc acaaggcgtt caaaatgtgg gaactggagt cggtgtaata ataggagggg
    32581 gttcccatgg cggacgatat ccgccagagt cttccggcga aaacggccag cacaacgacc
    32641 gtcccccaca gcagcggcca cggtttaacg atgggtctgg ggaggacggg aggcacgggc
    32701 ggcgggccgg ttcaggcgtt ccaaaaaatt cccgggagaa ctctcatctt ttgataggtc
    32761 gacaaccgcg cacggccgag aaaaacgttg tagccccgct ttttgatctc ccccaggagc
    32821 gcccggtaga gccgggccat gatcaacgcc ggacgggcgg cgcgccggct ggtgtccgga
    32881 agcttttgca gggcggcggc atacgcttct tccgcccggg aggcctcgaa gcgcatgagc
    32941 cggacgaaat tgtcgttgta tacggatttt ttcagttcct cctctccgta tccgaaccga
    33001 ttcaaatcct ccgccggaag gtagacatgg tccaccgccg catcggcggg gatgtcgcgc
    33061 aggatgttgg tcaattgcac cgcgcggccg agggtcacgg cgaactcccg gtgggactcc
    33121 tcgtccaacc cgaaaacggg aagacaggcg aggcccaccg tccccgcgac cccataacag
    33181 tactccttca attcctcgaa agtcccgtat cggttcttca acaggtcccg ttcaacgccg
    33241 tccaccagtc ccaacagcgg cgcggcggcg gtgggataac gttccaacgt ctcgaggaga
    33301 gccggccaca ccgccgcgtc cacctgtgcc gccgattgtt ccagcgtccc ctctttccgg
    33361 gattcgacgg cggtccggcc gtcgcgcaac gcgcgcaaca attcccgcca ggcggccagg
    33421 gtcctttgcg gcccgtccga cggccgttct ccccgcgccc gacgcagcac ctcgtcgtcc
    33481 acggtatcgt ccacggaacg gcaaaacgcg tagacggctt ggagcgcccg ccggcgcggc
    33541 gcggaaagga aaaagaaggc gggggcgaag ttgctggatt taaaggaatc gaggagggac
    33601 atcggcggcg ctcaggaacc ccggggcaag acggccgccg gattccaaaa aaacaaccgc
    33661 tgcatgagac gcggaagatc ggtcttgccg agggtgggcc gttggcccca cacgtccccg
    33721 tcctgcgcgc cgatcttttc caatatgcgg gtccctccca accagaccag ccgaagctcc
    33781 cggcgcagcg ggttcggcac gtcgtcgcag agcgggacgc ccgcgcggaa aaaagtccag
    33841 gtcctttcga cctggaacgc gatcagccgc cggagcggtt cgatgaccgg accgcgcagt
    33901 atctgttctt cggcgaggcc gtgcgccttc atgtcctccc ggggcacata gatcctgtcc
    33961 ttggccgcgt ccacggtcac gtcctgccaa aaattggcca gctgcagccc ggtgcagacg
    34021 gcgtccgacc atttcccccg ctcttcgttc caaaacccga aaacgcgaag gaccagttcg
    34081 ccgaccgggt tggccgagta acggcaataa tccagcacct cgtcgaaatt ctcgtggcgg
    34141 ttctttgtga cgtcgcgctc gaaagcggtg atcaggcgtt cgaacggatc gagggggagc
    34201 cggaaccgct cgaccgcgtc ggccacggcc cagaaaacgg ggtgtcggtt gtgggaggag
    34261 ccgcaggcgc ggagccgtcg ccgccaatcg tccagcaggg agagacgctc cggcggagtc
    34321 caccccggct cgtcggcgaa atcgtcggcc acccgggcaa aggcgtagac cgcggcgatg
    34381 gggccccgct gttcggcggg gatcatccgc gacgccacgg gaaaattttc gtaatgggac
    34441 cggcaaaggg acagacaggc gccgtacgcc tccggcaagg aagccgcggc gagggcggac
    34501 gaagccaatt gcgtggacgc ggggagcggg gtcatcttta tttctcggtc agagtaataa
    34561 accacttttg gcgggtctcc cgcaagtcgg ctcacgggcg gcagggggcg gcgacgcccc
    34621 ggcctctggc cggggccgcg gccgccgaaa aaagtcccgg gtcgagcggg ttttccgcgc
    34681 ggcccgggac gggctcaaac caacggttct ttcctggaga agcttatttc aggtgcttgc
    34741 tcaccagctt ggtcatttcg aacatgctga cctgcttttt cccgccgaac acttcgccca
    34801 aggtggcgtc ggcgttgatg ttgcgcttat tgaccttgtc ctgcaggctg ttcttcttga
    34861 tgtagaccca gagttttttc accacttccg tcctcggaag cggagcggac cccacgattt
    34921 tcgccagcgc ttcgtccggt tgcagtttct tcatgaaagc tgcgtttgcc atgttatttg
    34981 atcctcctga aaatatgatt tcgactaccg gcacaaacgg tagcaaatcc ttgccccgtt
    35041 gtgtaggggg cttcaaaaag ggggcggggc ggctcaaccg ccgccggttt ttctgttgaa
    35101 gatggaccgc atgaacccgc cgccgctttt ttcgtgggcc gggcggccgg attccggatg
    35161 tttatcgtgc ggcggtttgg cgccaggctt ttccgactcc ggcggtttcg gcgggatctc
    35221 cgccttggcg agctgcttcc tctccgcctc ctcgtcctgc ggcagatggc atttcttgta
    35281 tttcttgccg gaaccgcaat gacagggatc gttgcggccc agggtgccgg ccatctcccc
    35341 ttttgcgggg gtcaatttcg acagaatttt tccaaacgcc atggtgaggt tctccttttg
    35401 ttcttcagcg gtcttcgtcc caaaggggcg cgtgccagag cgtctccatc agccaacgga
    35461 tctcggagtc gcaaaaaacg atgtcggcgt aatcgggttt cccgtcgtcg tccaggaacg
    35521 tgatggcgat ggtcacatcg cggtgttttg ccgcctgctt gttggccgac cacgcggcgg
    35581 agacgcagag gatgccggcg gggatgtcca aatgggcgcg ccaatcggcc ttgcggcagg
    35641 gcgcacgaca gaccagccca tgggagccgc cggccgtctt gtttcccgac ggtttgccgc
    35701 tcatcccggc ctcttcacaa agagaagaga ggaaatggtc cagctttttc cgggtgagcc
    35761 gcggcatcgt ccgccgcgaa tttttcttgt tctttttcct tgaatccggg gtttccatcg
    35821 cggaactcct gaaaaacacg ctttgacgcg ggaagaacgg caacatcccc ggaccccccg
    35881 cgtaatttcg ttattttaac atttcatccg ccgcccgcaa gcccttcggc cttttttcca
    35941 aaaaaatagt cgaaatttat ttttgaattg acaatacaag ccatttttga taaaaattaa
    36001 cgttccgaat cagtcttatt ttgttgcatc ggaggcctga tgacgctgtc tttctggcac
    36061 cggtggcgga aagaattcgc ccggcgcctg accattatgg tgattcctca cggaattgcc
    36121 cgccctcgcc aactgtcctt ctccgtttcc ttccttttgt tcctctttct ttgctggacg
    36181 ggcgtcaccg cctgggcggg ctacatggcc tcccaacggt tcgattattg gcgtctgaag
    36241 gcgaacaccc acgttttgcg catcaaagtc gagtatttcg ccaacgaggt caaacgcgcc
    36301 cgcgaaatgc tggacgacgt gaaggagatg gagacccagt tgcggaccat gatgggcatg
    36361 ggcagccgcg acgccatcat ccaaagcgaa gagaaccctc tgcagaacca gggcggtccc
    36421 acggcggggg acgagatgga actgcaaaaa ctcctggacg gacgcgtctc cgaattgacg
    36481 ctgcaggacg tttccctcca gatccaccag ttgcgccaag agatggacca gcgactttcc
    36541 agtttttccg aacttaccga aaagatcgat tatgaacgcc ggcttttccg ctcccttccc
    36601 aacatctggc cgaccaacgg ctatctaacc tcccatttcg gccaccggct ctcgccgttc
    36661 agcggcctgg acgactggca caaaggcgtg gacatcgccg ccccgcccgg cagtcccgta
    36721 cacgcctcgg ccgacggcgt ggtgatcctg gccggctggg ccggcggtta cggcaaggtg
    36781 gtggtcgtcg atcacggcta cggctattcc acccgttacg gccacaacag acagatttta
    36841 gtgaaacgcg gcgaccgcgt ccgccgggga cagatcctct ccctggtggg ctccaccggc
    36901 aacgccaccg ggccccactg ccattacgag gtgtggttcc tgggacggcc ggtcaacccg
    36961 cgtcgattca tgcatctcga aggctgatcg ggggacatca tgttcggaaa aatgaaggaa
    37021 gcagacgcgc gaatggaaac cgttttgggg atggagaccc actttcaggg gaccatccga
    37081 tccaaaggct ccatccgggt ggatggacgc gtggaaggcg gcatctccgc cgaaggggtg
    37141 gtcatcggcg aaagcgggca ggtccagggc gacatcaccg ccaagaacat cgtcatcggc
    37201 ggaaaagtca ccggcaacct catcgcctcc gccgtgctgg aactgcaggc ccacagccag
    37261 gttttcggcg atctccgaac cgcccaactc tccatcgcgg aaggcgccat tttcgagggg
    37321 aattgcgtca tgaccaccga aaaggcgaaa gtcatcgcga tggacgaggt gatggcgaag
    37381 aattcccggg gctgagattt tcccggcatt tttcaaaaac ggctggccga cccgtggccg
    37441 gccttttttg ttccgctcga tccgatgacc aaattcctcc ggcaaaggtg agacctcctc
    37501 catgatgaaa tggctgcgca aacaccgcta caccatcttc ctcatcaccg tgggcgggtt
    37561 cctcgtgggc tccttcatgg ggttcggcag ctacttcttc acccagtccc cctacgatgc
    37621 cgccatcgtc gtcaacggcg acaagatccc ctacaaacgc tatgtgaccc gctaccacca
    37681 atacctcaac cagcgccgcg acaacaacga accgctcaac gaagagaaca tcaaacgcat
    37741 caaacaagaa acccttcagg acctggtacg ggaggcggtg ttcctccaag aagccgacaa
    37801 gtacggcctc acggtgacgg acaacgaggt ggccgcgtac atccaacgca gtccggcgtt
    37861 ccagcgggac gggcagttcg accagggagc ctatctgcag gtgttgaccc aggtcttacg
    37921 gatccccgcc gatgagttcg aagacgaccg gcggagggag ataaaaatac agaaactcca
    37981 ggccctgatg gcctccgcgg tgaaagtttc caacatcgag ttcaactggc agtatcagaa
    38041 aaggatggcc ctcggcaacg ccgaggaaaa gaaaacggcg aaggacaagc cgaacgaatt
    38101 tcgggaacaa ctacggcagg aacaggtctc tcatgtgttt caggaatggc tgagtcaaat
    38161 caacagccag ctcaaggtga aggtctatct ggacaaatgg gaagggcgcg gcgaaggata
    38221 ggtcccgccg cgacggtccg ccggaaaaac gtccttcccg tgcgtcgctc ctcgttaaaa
    38281 atcaaattcc ctttcgagac cgccccgccg aacggctcgg cgcataaccg gccgatcgcg
    38341 ccttggccag gagccgccgc agcccgcgcc gcggcagacc gaccacgtta tcgtagtctc
    38401 cccgcaattt ttccacgaac ggcgagccct tttcctgggc ggcgtaggcc ccggccttgt
    38461 cgtggttcct ctgggaccag taccgcaatc tctccggcga aagggcccgc attttgaccc
    38521 gcgtggacca gacacattcc caatgctttc ttcccggccg cgccaggagc gccaggccgg
    38581 tgtacaccgt gtgccaccgg ccggccaatt cggccagcat ccgccgggcg gcggccacgg
    38641 aagagggttt cccgaaaact ctcccccgac ggaaaaccag cgtatccgcc cccaaaatca
    38701 atccttccga aacggcggcg gccgctgggg ctgccttcag cagcgccaac cgcttgaccc
    38761 aaaccgacgg acgaacgccc ctcttccaac ggggctcctt cacgcggccg ggccggacat
    38821 gcaccggaaa agggaacgtc gccatcagcc ggcggcggcg gggcgaagcg gaagccagga
    38881 cgaacggcac ggaaaaccga atcttttctc tccccatcct tctcggtcga cccctttctt
    38941 tcgaaaatac cttcc
//
LOCUS       DAOTFD010000004        38532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_708_length_38532_cov_5.031655, whole genome shotgun sequence.
ACCESSION   DAOTFD010000004 DAOTFD010000000
VERSION     DAOTFD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 38532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38532
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_708_length_38532_cov_5.031655"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5079_00550"
     CDS             <1..393
                     /locus_tag="P5079_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28504.1"
                     /translation="YPERLRRYEEFCRLALELGAPETSGVLSVKPDKWFLLGDYYRHR
                     KQFVRAAACYLRSWRRESRNKEVLVKAAECCRELGDAARARRYYEKALAINASVAGFG
                     EDPYDAVIRKALESLGPAAKSSSPLVRA"
     gene            417..1853
                     /locus_tag="P5079_00555"
     CDS             417..1853
                     /locus_tag="P5079_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28505.1"
                     /translation="MVDALRKLDADRRSAAERRFVRPVLGFLGKLRRRYLPELDENIV
                     RLLWARRAWRKLANFLLVELQAENMGTRVTGKPYWLTIDPTNFCQLRCPFCPTGGERH
                     IRSKAVLKLEDFKRLMDELGPTLLHVDFMNWGEPLLNRYIFEMVSYAKKFDIDTKMDT
                     NLNEFSEEMAEKMVRSGLDCVSLSIDGLTQETYSKYRVRGDFDKVMRNLETLIRKKKE
                     LGSSRPYVIWQFLVFKHNEHEVERVRQLGKEMGVDVVGVTPAFLPFRPGIKDEWLPLK
                     PEHRHYDPETFPDRPPWEWESAPKTEAPSLPDPAPVAPLPEEKLSAAAVPHVEVLPPR
                     GGVRSIIERFFGRKRGGAGKEETPVINVQVYKEPEKRPRCNWPWAGIAVNPNGSVSPC
                     CSVEEEIYDFGNIFTEGFSGIWNGPNYRRARRHIRDYVKHRQDVKTRSEHACERCFSI
                     GRANFTFQPWWRFSQFDWWWGLREKDPR"
     gene            1875..3032
                     /locus_tag="P5079_00560"
     CDS             1875..3032
                     /locus_tag="P5079_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28506.1"
                     /translation="MQDNALLMTDEHVQRKLNIALNRLEFEQKRAVVSSTPEVITIGA
                     HNHCNAHCIWCLEGDISVFDLDRYKEFFEKSLGHVIRKARHVSFVGFGELLWLPRVEQ
                     FLDHLNETIPDVVKQFTTNGTPLRPDKIVEKLLVGRYSIRISLHASTPELHAKITRIR
                     ELDRILENLRYLVKLREHFQRRKYLHVELFNVLTKDNIDNLPDFVRLAAELDLPQVVC
                     SYMTIFSKEHIEMSLFFDQQRANDRLAEAEEVAKKLGVMVQLPPRFGQGGRDRERPAG
                     ECSDPWQYFYAETQGSVLPCCYAGGHVGYLDKQSFEELWNGEFYQNLRKGLAGEMPAH
                     EWCKNCVKFKGHYTVNNVLCHITNRPETQKMIVEEMERRGLLKPTAAGGEV"
     gene            3032..3943
                     /locus_tag="P5079_00565"
     CDS             3032..3943
                     /locus_tag="P5079_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28507.1"
                     /translation="METTPPTSLERPQRQVVFAWDMHYSCNFRCPYCFYTLTGWHELA
                     KKSTYKSAEEWKRVWHRLYEKYGSCHIRITAGEPFTYPGFVDVLAALSQEHWVQVTSN
                     LSLRPLLEDFVRRANNRRVELDCTFHPFEAKFEDFLQNVLLLRGRGFIANVCYLAYPP
                     QMARMEEFKRRFKENGIHMNMAVLWGRYAGKEYPHAYTPEEKRWIKEVIGYEIGPETV
                     GLEPIPTKGKICGAGQRYAVIHADGRAVRCGQLGEETIGNILDENFELFQTGRPCPSD
                     YCRCKEFQMAWEEEEKQALLQRGEVKV"
     gene            3969..5624
                     /locus_tag="P5079_00570"
     CDS             3969..5624
                     /locus_tag="P5079_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28508.1"
                     /translation="MKIALVQCPVWGVADPPLGPAQLAGCLKAAGHEVRVFDLNVTLW
                     RKAPPRHANLWLWEMSRFWNSPEIVDRFFADNRRLIESSVQAILRSGARVVAFSVNDG
                     SQWASLKVSQWIKEADASRVVVFGGQYFFKGTAAEEILKVSGVDAVLRGPAEESLPAL
                     AGDVETFGRPRRRPGLLFRENGSLVDGGPASVPALLDKLPFADFTGFPLKLYAEQGRI
                     PLSASRGCVWACHFCSAGRFWPNYSYMSGDRIFAEVKHQRSLFPSRRHVEFYDITANG
                     RPEALRRFSELMIEERLAGRPAGHTGWKINAIVRPEMTPDLLRKMKAAGCLSVIYGIE
                     SGSPGVLKRMNKHYSLPLAERVLADTHAAGIHTTANFMVGFPGETEEDFQRTLDFLKR
                     NKRSLDRAYPSATFTSLEEHSPLAENYAAFGIESENGRRPHNLYWKMPEGNDYPVRLE
                     RYKKFRALALRLGIDRAKGLHGDLASHAARDLAAYHRYAGAPLRALALYRAYLRRDPG
                     NGPVRHEMEELLMEISGKKASAAVPRGCKKRRTTSRPIARTVA"
     gene            5621..7591
                     /locus_tag="P5079_00575"
     CDS             5621..7591
                     /locus_tag="P5079_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28509.1"
                     /translation="MKVALVQMPVWWTLDPPLGLAQMAGCLRAAGHEVSVFDLNIQLW
                     RRRSPQSHALWSWEQFHFWNYEERVDKFFKENRAALESFLESILKTDARVIGFSVNIG
                     SQIPSLRMAEMIKKADPSRLVVFGGQYFFLGDKTAEMLKNPPVDVVFQGAADESFPLF
                     LTDYARTGRAVLRPGVGLRQGEKIVLGGAVSPVPQLDKVPFADFSLFPMDLYEDQGRI
                     PFAASRGCVWACHYCSTHRFWNGYSCMSGDRMFAEILHHRRLFPQRTHVDFYDITANG
                     RPENLFRFAQLVRDHHQRSTLMTIGWKINAIVRPEMTPELLSALYEAGCMEIIYGIES
                     GSPRVLKRMNKNYSPSVAERVLRDTHAARITTTANFMLGYPGETEEEFGMTLDFLRGN
                     AASLDKAYASATFTSLEENSYLTEHRAEFGIRDPGLKVGHSLYWETEDGLNSYLVRMD
                     RYVRFRALAEQLGLEAYKGINGEVELERLSKLAQYFLYTDNRLRATEMYLQYAQRAAD
                     SETARGDLREFQRELEAFPQALRRFERWRNGHSEVPASAQETIWEKANAWLTDDKPFR
                     LEECPQLAEWLRENGRYKDYVDDMLETHAFLQGLKCHAFLRWDKGFRLFWGRDVVEGP
                     GRVAGLLEEVSAALGQDASGGKVKDERMVNSR"
     gene            7588..9549
                     /locus_tag="P5079_00580"
     CDS             7588..9549
                     /locus_tag="P5079_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28510.1"
                     /translation="MKVALVQAPVWWTIDPPLGLAQMAGCAMQYGHDVSVFDLNILLW
                     KDRLPKYQNMWLWEQFHFWNNPQVVADFFNDNRRAIEGHVQSILRTDAKAVCFSVALG
                     NQLASSMIARMVKKEAPKKWVVFGGQYFFRDNQKAKEVILLDKNVDIVVRGASDHLFP
                     KLLSVLETRGEPRPIRGLLYKQGRVVTDTGFIEPLRQLDSVPFADFTKFPMEVYEDQN
                     RIPIAASRGCVWACRFCSSCNFWPGYSYMSGERIYAEILFHRRLFPQRFHVEFYDITA
                     NGRPESLSKLCDLIIEHESRTGWKHFKWKINAIIRPEMTPELLMKMALANCQDIIYGI
                     ESGSPRVLKLMNKHYNVDVAERVLHDTHAAGIKAVGNFMFGFPGETEQDFQMTLDFLR
                     KNQKSFDRVYASATFTSLEEHSYLTEHQESFGVVKSEDKHNSLYWQNKDGSNNYLVRL
                     DRYKRFRDLAIELGIDAYKGLAGSVELDHVLNRANYYEYMDHRYQAVSAYSDYLAMDP
                     MNEPIRHRLREFQKELAGLARGFELLRRWEREQGVSNPLGREAWRALSPHLLKERPIR
                     AFVLGRKAGAAVRSSRGRKYLKALLALHNALQKLKCRATLRRSGSRVRLFYGTDLLEN
                     EEQVAGLTKRVDRIVVAAPGVSGKGGVPG"
     gene            9546..11585
                     /locus_tag="P5079_00585"
     CDS             9546..11585
                     /locus_tag="P5079_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28511.1"
                     /translation="MRVAVVQAPVDWADVPPSRLSQIAGCARQYGCEVVWCDLNAIFR
                     KRAPESARPGWGWSHFLFWENSDVVARRFREMRDVVREQVERLKEADVVYFSVTKFSL
                     RASLLFARRLKESAPKGLIVFGGPFFTVGNLRMHEFITNEPDVDVVLQGPGEFLFAKL
                     LRDMKVFGRPNLQPGLHYRHGKELVMGDPVEGFGDMDALPFADFSGHAPELYDDGESI
                     PIRLSRGQSFRCPACGAEEPRLRPRWMRGERLYAEVLFHRRNMPRHTKLLFEDVVDEE
                     GARTLDSFSELMCQDAAKALSPAAWGAELDLAATAGRGSLATWARSGCRRVTVRFQER
                     PLTDAEKRALLEFFEHGRACGMKMVPKLFFAAPGQEPRDVRDQAEFVAGNARFFQDGF
                     EMGSLFYDALAVDVQLQHEMEKTAVGRSDPSPCHFGFGDRWTRSGDDLRSDPAARCLE
                     LKSVLRRADLSLLENRRETVENDYALMRNQIWRWHGHPIRQLEGLLAFFERESEDSIY
                     HQAVIKENAFLGRIWERLSAAARLASAAQFYIAKAFNSPDWDPSDWEIPEDIGRVLRE
                     GLRRDCLERLRRRGIRRGNRVQTIHRYLLLIRALLGRSNALGDVKFDFDVPANRFRIV
                     WENDDSDMAYAGDLGRKLLSILRKTKKLAPATRPGVPAILSPKGAPGRLNVPGDH"
     gene            11602..13494
                     /locus_tag="P5079_00590"
     CDS             11602..13494
                     /locus_tag="P5079_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28512.1"
                     /translation="MSLAFVSLPFQDTGLPDVRLAQLIGIARQMGQDVSVHDFNVRWR
                     RRLRKTEKKFWLPDSRVCWLEKVLARRFWEDLKGEFQADVDGLLDGNPSRVFFWITAS
                     SLQLSLLLAAEIKKRARRPKIIFLNPHLFLGAFMDEDHRAISDFLQTEPVDGILQGPL
                     YNDFARILTQREELLGSASGFHCVKDGMVHLGKKPTVEKGFDSLPFSDYSLFPLHLYD
                     DKDGFPMQVSDGCACDACRRAGFGPDAILASGMRIFSEAAYHRRMLPGRHQMKIVRPA
                     GEKVFPSLQKFAELLSADMEAVGKKYLEWEFLTREPEKMTSEQLGWMIGAGCKRIGVV
                     HRTGRGLPPCPPEPPRGRRIIAELHHYLVEEDDGTRDSVPADMGPSGGYKKIIRHVVE
                     QDGLDAHYLRNLSRMDYELKSGRPGGDGELPQEISSCARGPEGREEGLSRSALAMASF
                     LNARKILFDRRHPFDAFEFFRSLSPELRGGRLMRLFYRHLATDARRMMSMADRLQAAE
                     QIFNLLPQESRRKAWMGSPTFHQEGDHSTYCRHRMGMKVFKDYMPQRGERLLIAALAR
                     ATQIRMLRAAVVLDEDRNEFLIRWGESILPASRVRLLCRDFLSLGLGVAAERASAGPS
                     PAGVPA"
     gene            13491..15446
                     /locus_tag="P5079_00595"
     CDS             13491..15446
                     /locus_tag="P5079_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28513.1"
                     /translation="MKVGLVQSPVWWTIDPPLGLAQVAGCLRAAGHEVAVWDLNILLW
                     KNRLPQYENMWLWEQYQFWNHPDIVDRFFEDNREFIDKTLEGMLSSDCRLFGFSIYAG
                     TQRAALRLAQRLKEADSRRRVVFGGQYFFRGPHAEEMLRTGFVDAVVRGAGDELFPQL
                     AADLERTGLPRKRAGLLFRDGADIVDGGPAESPKNLDALPFPDFSGFPMDLYTDKTRI
                     PVAASRGCVLGCRFCSSREFWPHYSGMSGERIFAELKHQKAMFPQRAHFEFYDLTANG
                     RPENLYRFALLAADFMIQHRPERNFRWKINAIVRPEMTRDYLAVLKAGGCQDIIYGIE
                     SGSPDVLWRMNKNYDPAVADRVLQATHEVGIRTVANFMFGFPGETEEDFQLTLDFLRR
                     NRPWLDMAYGSATFTSLEEYSYLAEHPREFDIRQETAHTAHNLYWDSLDGKNTYPVRL
                     DRYARFRRTCRELGLDAYKGLDGALELDRLANLAHYYQYRDRRLEAIGAYVDYLERDR
                     ASRPMREQLRRYQGDLRLLVKALRLVERANRLLLSANGALREGQELLRRTLESGELLA
                     MPGSSRGEIDRVRLYVARAQRFLRRLTHGGGLRWGDRGLVVCWEKEPAPPLEELRALR
                     SKVDRLIELSESEEARTSPAAVVPGAR"
     gene            15471..16619
                     /locus_tag="P5079_00600"
     CDS             15471..16619
                     /locus_tag="P5079_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28514.1"
                     /translation="MNVPNTTVEPGSPRDLRLKLLAEKAEDNIRRNNEEYAQRRVVLA
                     STPQNIFVQINAVCNANCVFCSKGYDYPLFRLDDWLKHYGDKITPALAQARQVILTGS
                     GEFLGLPDAQRILEYFNSEFPHVEKYVATNATYLTPKIVDLICSGSSKYVVQLSLHAS
                     RPEIQRVMMRYGAYDRVQKNIRALMEARDKKNVNVLFMFIMTTLNIDDLPDFIRYASR
                     MGADKVVCGYFYIYESNQKYLSLYFMQEKANQRLEEARRVADELGITLQAPPKFGLTP
                     EVYKAFDVCQEPWYQFMMSNDGYVLPCDAYGSFPREENLNVMSFSELWNGPTYRKIRT
                     DIVDGKGCLLTCSRHNPKSVNDWNSHVIHRFKEDRQIIKEHHESLRRP"
     gene            16647..17705
                     /locus_tag="P5079_00605"
     CDS             16647..17705
                     /locus_tag="P5079_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28515.1"
                     /translation="MMTDPPSAPLYPRRHAPFRVHWNWYLLQQCNYGCSYCPEVEYKR
                     TKTAPLPILNVSLDDWTRIWDRLFGLYGSALIRFAGGEPFIFPHFIDFLAMLTEHNAI
                     DVTTNLSFDVDEFLRKVTPDALAISASYHPEHVSLGEFLGKLERLKREGFCVSAALVA
                     YPTFLGSLKKTIEGFDRADIQLKLIPFRGKYQGRDYPLAYTQDEKDVMRLATQTRNTD
                     GNVINAQWFREMVEKEEKRQEEIRAYNERRPDPSPPPAPVPESGNGKSAPPLCRMGEN
                     YAKIYADGTVRRCCCPGVPVMGRIMDKDFRLLDAAGPCDVPCLCWKPMKLGEYESRWE
                     TLWQGLVHRKHPLKTEGD"
     gene            17718..18833
                     /locus_tag="P5079_00610"
     CDS             17718..18833
                     /locus_tag="P5079_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28516.1"
                     /translation="MTDGTAASQSGVSENGGAATMPSAGPRLSEFHRTMPVFYNWEIH
                     YSCNYRCSYCPFTVSGWHTFSSKNAYPPLSRLESAWRRLYERYGKGHIAISGGEPCTY
                     PEFVPLVQTLTRMHSVELNTNLSFDEEKFAAAVDLKRIRLAASFHPQFAAFEDFFKRT
                     VYFKERGVDIFVNYVSFPEQLEEMWRYKKEFQAAGVPFYIMPFNGKYEERVYPQGATE
                     REKACYDAAMNGVDPETWINRQRREWPGHPGQYFPNGVTPSEAARLERQGEKRVVPPV
                     VAPELAAAAKRAPVLCLMGYRYCKIRPNGETYRCCAWLAGNTDLPKPIYLGNLYEDEK
                     FSLLEEPAMCDYTPCPCERCMVVGEEDRWRHRWVTTE"
     gene            18847..19713
                     /locus_tag="P5079_00615"
     CDS             18847..19713
                     /locus_tag="P5079_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28517.1"
                     /translation="MEKAAQNRHPQVRFTWNIHWSCNYRCSYCFFETHWEEYGKRNVY
                     KSVDEWMGYWERAAELYGRCFITINGGEPFTYPGFVDLIARLSRVHWPINITTNTSLH
                     LKEFVKKVDPERVSLSVSYHPEYHRVDDFLEKVFFLRQSGFVGGCVNFVAHPNHIGTL
                     PALLEKFVGIGESLKVNPFIGEHAGRRYPDGYTPEQKEILGMRDSWMDDKRHKGMLCR
                     AGNLSGLLLPNGNVARCGQIGDRHIVGSFFDPKFRLLPEPLPCDVEFCPCDEWKVVPD
                     EKEPENAGAWLP"
     gene            19745..20635
                     /locus_tag="P5079_00620"
     CDS             19745..20635
                     /locus_tag="P5079_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28518.1"
                     /translation="MRSRLSFTWDLLYHCNYRCSYCWWDPHWLKFAEKYAGYPSARQW
                     IRAWENVRRLSGEADISVLGGEPLMYAGFSDMVRALTTAQGHHVCITTNLYTGSRQLA
                     DFVKGISPEKLDLSCSYHPHFASLTEVLEKVKALKELGFHVPVYLISFPPFLEELPSY
                     EETVRRAGGDFRLRIFCGEYNGKTYPRDYTDPERALLSKMLGNPVQVDYQIDQKTTLG
                     ERCYSGAVYGNVKPTGDVYRCGQSAIDGKPMGNIFDEDFHMLPGPTPCPFTHCSCQEY
                     EYVAKEYERKRALRAGMSVA"
     gene            20672..21811
                     /locus_tag="P5079_00625"
     CDS             20672..21811
                     /locus_tag="P5079_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28519.1"
                     /translation="MSDQERAAHVRAWFEGEPAGPFTIQLNPTMACNLNCLFCRRQNQ
                     LEAFYRDHRELPDQKYMEIVEEAVALGVKAINIKGGGEPLIRRALMRELVPLIRRNKV
                     HGSLITNGTLLDEPLAELMVRWEWNELSVSLDSPEAATHDYLRDKPGTFDRLMAGQRW
                     INEFKRRHKAALPHVKWHSVLTNRNHRRVADMLRLAAENGAAEVEFDSLDLSEPSSAV
                     LKLDEGDVREFQEGLPPVLALAERLGIRHNLQNFDRRDYAVRGGANGVKTGDKTSPAP
                     GGSPYERIPCFFPWFHCDITPDGSLVPCCYGEGYESRENLHRMSFREAWRGRGMKEYR
                     DSMRDGTMKPFCRNCTACYRDQNDRIREALRCGPAGDAPRREKVF"
     gene            21853..22785
                     /locus_tag="P5079_00630"
     CDS             21853..22785
                     /locus_tag="P5079_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28520.1"
                     /translation="MSPNRPMDYFWVVMTQCDCRCPYCIYGLYPLDRRKVGYFYTAEE
                     WLAGWERMYKKYGEGRIVLTGGEPTLYPGFTDLAVQLSRWYQINIDTNFQLPSAELEL
                     LLKRLSPRRVDFSTSFHPFMAKTDEFIAKIHLLKRNGFNYLCRLVAFPPLVEKIPAWR
                     KTFGDEDIILFVNPFSGEWNGRKYPESYTPAEKEIIFGQKNSRESARIHPADKEFVRQ
                     MLNMESPRGRPCRTGSEHVRVEDNGWVYRCVSYAAMEREPLGNFIKDDIVLWDRPRVC
                     EADACEWEYRYLVDETDRFKSDKVLRDATTRGGV"
     gene            22788..23633
                     /locus_tag="P5079_00635"
     CDS             22788..23633
                     /locus_tag="P5079_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28521.1"
                     /translation="MGQGPENRTTFGWDLTYVCNYRCPYCTVWDQPTPIKDMDVAAWR
                     KIWDRFYELYGECYIYMSGGEPSAHRCFFELVDMLSKKHIVDICTNLSWDVGRLADAI
                     PPGRLRIAATFHPSQVLFREYVEKAVRIKEYLPLRPEGPTVYYVAYPAQIPEMEEHAR
                     LFRERGLALVPLPYVGHENALGNSADEKEKIRAISPNKGDPLKKLEFQLKELSPKGRL
                     CRAGQKYAMIRGDGSVDRCSQYRAHQMGDIRDANFQLWDSPRPCTLAWCPYESQWLVR
                     EETRS"
     gene            23630..24640
                     /locus_tag="P5079_00640"
     CDS             23630..24640
                     /locus_tag="P5079_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY28522.1"
                     /translation="MKGRRRFWGGRFLLVAAGGLLPLLVAGIAEWNLRRDGFQFEPWR
                     YYRRFGANFEAEEIESYAKDPDLFWRFRPSQTLRTWWVEEATINRYGFRGEDFPEKKE
                     PGVLRVLCAGDSGTFGWGVRDNESFSVYLQEFLGRRWGEGKVQVVNAGVPGYSSHQGL
                     HVLKKWGPRLRPDIVIFSFGRNDHNPTAWLTDRQRKKIPRPLVELQNVLLRTRLYQWF
                     YMRTTLYMYIEHREKFLGSRRETVRVTLSEYEENLRRAAALCRDGGADIVFVPRSQQM
                     AYIDVMKRVAKETGSRYVDLEKFSSDRGIKDYSLGEHPGWPAYKRLAEIVGEEVARLK
                     TP"
     gene            24653..26332
                     /locus_tag="P5079_00645"
     CDS             24653..26332
                     /locus_tag="P5079_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28523.1"
                     /translation="MKPEMAEELIKKLYRVGPAGVLVDGKRVLYFSPAGLPAGAPPAD
                     VIFIPGESSRHRSFPDIKKIATAQTIVVGRPSHISRFPCNLLSVEAEKKGAVLGMPIV
                     SRKNPAAPGETDFLVTVDGLRLAYVASLSGGSSAFGEADVVFSSAPEKEVSFQQAISA
                     MKRMRVRFFVPLGDSRLPDAFVQRLEAEKIGVLQLPGLNGKNRPAGISKEKTSRPAGP
                     STTAVGTLYRPKTAADRITFTWNIHHSCNYRCPYCFMATDPIHTFRKNSYPALDQLVG
                     VWERMYRLYGSCVLKISGGEPFIYPHFVELLEGLSAHHFLDFSSNLSWDVRGLIGRLP
                     PRSVHIDPSFHPGFYKDIEEFAEKCLLLKKQDSLGSVHIVAYPPFLQKMRGYKEVLQR
                     KGLDIVFLPFRGRYRDKQYPESYSPAEKELLRDLARSNRDETKNPPAASAPPAAAKPF
                     IEKVNEQYFSWFVENKNAPGPQGGRLCWQGARYGKIYSNLDVYRCCTREDSPELYVGN
                     LLDPEFRLLDGPRPCPMTDCACWKAMLCGQENTWKTLWNTENFPMPARLKT"
     gene            26329..26499
                     /locus_tag="P5079_00650"
     CDS             26329..26499
                     /locus_tag="P5079_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28524.1"
                     /translation="MNGAGAWDNEKMWEYGKRGMLRDIKDWCTVDGALGRLGPKQFSE
                     GLIAGQGDAVEN"
     gene            26517..26708
                     /locus_tag="P5079_00655"
     CDS             26517..26708
                     /locus_tag="P5079_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HRY28525.1"
                     /translation="MNCDKAKNMKSCNCTYPCARKGRCCECLEYHRRSGELPACYFTA
                     AAERTYDRSVENYLRSRGG"
     gene            26729..28261
                     /locus_tag="P5079_00660"
     CDS             26729..28261
                     /locus_tag="P5079_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRY28526.1"
                     /translation="MLAKISSATVQGIDGFLVHVEVDLSAGLPSFSTVGLPDAAVRES
                     KDRVVAAVRNSGFDFPIRKITVNLSPADIKKEGTAFDLPTAVGVLAAQEAVPSARLNG
                     YVLVGELALDGQVRPVRGMLPIALAARDAKCAGVIFPANNWDEASVVDGVDLVPVSTL
                     TETVRFLRGDWRPALPTRVPSAGGGNGFCGADFADVKGQIFAKRALEVAAAGGHNVLM
                     VGPPGSGKTMLAKRMPGILPAMGFEESLQTSKIHSVAGLLGRGHALLSARPFRSPHHT
                     ISNVALVGGGNFPRPGEVSLAHHGVLFLDELPEFNRNVLEVLRQPLEDGCVTVSRAAS
                     SLTFPARFMLIAAMNPCPCGYYGHPERHCVCTPFQVQKYRAKVSGPLLDRIDLHIEVP
                     ALRVSELTEGAPAGESTEVLRERVMAARARQGARLSAAGLFCNAQMNARQVKEFCAVD
                     AACRELLKMAVSRLGLSARSFDRILKVARTLADLAGVENIAQEHVAEAIGYRSFDRPP
                     EY"
     gene            28267..28614
                     /locus_tag="P5079_00665"
     CDS             28267..28614
                     /locus_tag="P5079_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28527.1"
                     /translation="MWPPRRNEFLERTKGVMAFGGLLVFFGGLGVVGHQLWYWLQFGK
                     WKKVPIWWALEKIGPPETSLGGWLAHPNSWYGLHKTLAVFSKTTPLSVTLLVVGGLIF
                     VVFISSMDSKRLE"
     gene            28835..29830
                     /locus_tag="P5079_00670"
     CDS             28835..29830
                     /locus_tag="P5079_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28528.1"
                     /translation="MLEKMERSYGDKGIDLSDLRKEKSRVVHDVRTQISASWAFYLRR
                     VKEGASNRERSYILEKMQKRYGESGANVGFVREEMERVEALEREKNVFGETVDAESVA
                     APAPPPEKTKTVRTQLRDVLFLQIEYLPDEDVLVRNKQDAQGMLPGLLAAGYDFADAG
                     THVADGIGCRLGLMWPVMKYFGWGGSGEYIIGPKEETAVNGVSRVALDGRLHRTTETN
                     FFRFLAQGEFRVPLYGRFSGRFGAGAGYATCKLSENTGNSGTFVSALGAPIHSKISER
                     WDGWTWEFSPGLVWRGDNVVLEAGIVSVFLPTMKQREDLNEFKWNPTGIRLRLQF"
     gene            complement(29850..29987)
                     /locus_tag="P5079_00675"
     CDS             complement(29850..29987)
                     /locus_tag="P5079_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28529.1"
                     /translation="MGFVVPQAQKVLLLETRGERAEIGVPEKGLKGWSRRNAVAPVLP
                     H"
     gene            30875..31240
                     /locus_tag="P5079_00680"
     CDS             30875..31240
                     /locus_tag="P5079_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28530.1"
                     /translation="MPVPSPYGAKTMTSNTMMADAKWKGDGQIISDVAKKALISQYDM
                     VYLNIGSEGGAVPRRRGDIYRKAQKVRAPQSGRPLGVVYRRVGVIEITQDVQKKSSAA
                     VVVTSHEPVKIGDIIKLRD"
     gene            31255..31638
                     /locus_tag="P5079_00685"
     CDS             31255..31638
                     /locus_tag="P5079_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28531.1"
                     /translation="MGRALALAVCCLAAVFWWHNFVSSGRCLAQLDAHPHRRGTATAL
                     FYFARYYEIFNKNDVALEHYKRIVERYPRSRYGMEAQYGVAASYEKMKRFHDAIVEYE
                     KFLEDYPDNKYTVSVRNNVEILKSR"
     gene            31781..32203
                     /locus_tag="P5079_00690"
     CDS             31781..32203
                     /locus_tag="P5079_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY28532.1"
                     /translation="MSFVRLVREQMKEKGLSIRQICRSADIDPSFFAKVLRGERRPPD
                     DEPVLRRLAQALSVDLVRLMVSVGRLPESWRAQCERPDVMAALNGLFSPERALSHPPR
                     PAAERTKKVSVPSAVRKAPPPERVPVRLPASALSEDLL"
     gene            32200..33336
                     /gene="dprA"
                     /locus_tag="P5079_00695"
     CDS             32200..33336
                     /gene="dprA"
                     /locus_tag="P5079_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRY28533.1"
                     /translation="MSVNLDERRARVLFNLADNMTPVRFRKILERFGSALAAYEADAE
                     EWDGLEDVSSAQARKLHRHCREAASRLPDELALLEKHRARALIETDEEFPVGVRALSD
                     APIVLYVKGDYRPADSLAVAMVGTRQSTPYGRAACEKLARELAQAGVTVVSGLARGID
                     AVAHATVLKFDGRTIGVLGSGLARFYPPENKPLATRMVERGAVMSEFPLATPPDRGNF
                     PRRNRLISGLALAVVVVEADETSGSLITARLAGEQGKDVFAVPGSIFSKYSRGPHRLL
                     KQGAHAVEGVEDILEAIEVFKNLSRPVKRPAPVQTNLTAAETALMECIRLDPVGMDEL
                     SVKAHLPASALSSALLGLELKGMIKALPGPAYVRSEHSLSSPPV"
     gene            33342..35492
                     /gene="topA"
                     /locus_tag="P5079_00700"
     CDS             33342..35492
                     /gene="topA"
                     /locus_tag="P5079_00700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY28534.1"
                     /translation="MAKSLLIVESPTKEKTIGKILGKNFVIKSSYGHVRDLPTKEFGV
                     DVEKGFAPHYVVLPKAKKIIPELTRLAKDAETVYLATDYDREGESIAWHLSEILKLGK
                     TKGRRITFHEITPSAIKEAMKHPRAIDQALVNAQVARRVLDRVVGYRLSPLLWKKVRR
                     GLSAGRVQSVAVRLLCIREEEIARFVAEEYWTLTAFLEKSGQVFSAALYGEGGKKFDK
                     FAFRHREPLDEAVKKLNGAAFTVSSVEPQERRRSPAPPFTTASLQQDASRRLHFSTSR
                     TMIVAQQLYEGIELGAEGAVGLITYMRTDSVQVAKEAQAEARQWIEKKFGKEHVPPKP
                     RVYKTKNKVAQEAHEAIRPTKPSRTPDEVRDFLTDEQFKLYQLIWQRFMAGQMADAVY
                     DTLTVDIGAAGYVFRAAGRTLRFAGFLAVYGESEDEKSQEETPPLPPLAAGDNLALKE
                     LKPEQHFTEPPPRYNEASLVKTLEEHGIGRPSTYAPIIQTILDRGYVRLEDRRFYPTD
                     LGKMVDKQLTDHFPDVVDVGFTAKIEDSLDRIASAEEDWKKVINDFYAPFVKDLERAE
                     KKMERVQIKPVETEEKCPRCSELMVIRENRYGRYLACRNYPTCRTTIALDRDGKKILP
                     EPTSELCPNCGKAMLMRTRGRLRFLACSGYPECRTTFSVDKDGNKIIRPKPEMTDQKC
                     EKCGKPMLKRVGKRGPFLACSGFPRCRNIKKIPA"
     gene            35529..36440
                     /gene="xerC"
                     /locus_tag="P5079_00705"
     CDS             35529..36440
                     /gene="xerC"
                     /locus_tag="P5079_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRY28535.1"
                     /translation="MELKEGIQRFLVYLRGERNASRATLRAYEADLSAFVSFVAARYK
                     SVRLLDCDRSLLRSYLAELQSKAAKRNTLLRKHASLRSLFRFLQRENYIAQNPMLALK
                     TPKRERRIPGFLSEKEVSSLLEMLPPRPQALATLRDRALLEFLYSTGLRVEEAAALNV
                     EDVDMWGASARALGKGAKERIVPVGETALRILREYLKMRKIDPLESSRVAPARPLFTN
                     PRGGRLSSRSIRSVVDRWARHAGLRQHVHPHMLRHSFATHLLDHGCDLRAVQEMLGHK
                     NLSTTQIYTHMTKERLRKIYDKAHPRA"
     gene            36433..37389
                     /locus_tag="P5079_00710"
     CDS             36433..37389
                     /locus_tag="P5079_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28536.1"
                     /translation="MPKGPLHQDKISLLMEETGCDQKQAELAMTSVGYNLEKAIRTIG
                     TLLKNIVVVRVKFHVPSQHLYGLVILLADVRRRSLFRIRAVVSYNPALYETPLAGDWY
                     DFEKTIYAFRLHDGTLQQVTQNLEKTLQEWFEKNSQEGLYDVLTVERIEGLREIFQKS
                     LFGYFTDNALEMQLSSDELNLEQFRRFKLEEETVPSRPEGGPDDHSGSKLVLNIDLQQ
                     DADGIPAREVKAGDFVYALLTDGRDIAQYLSKLLGGSGEHGLKPLPAPVESSRRDGET
                     ISFQIRLSGTIVGVAEVGADSLVRLQKRTAMSWWRRLVPFWE"
     gene            complement(37420..38208)
                     /locus_tag="P5079_00715"
     CDS             complement(37420..38208)
                     /locus_tag="P5079_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY28537.1"
                     /translation="MTRTPNSLSAAKIVLALLLGLAGTTPAQGEIFRYLTSSDGDDPV
                     LIVGDFEPPQTDKAPFLILLHGLGSTRGEWAPLVAQAAGRGWGTFAYDARGHGASRLT
                     RRGSTVDYRDPSFGQNPLFWRKMVSDLTDAVTALNEQKKLSKKRMILVGASLGANVSL
                     NVAALQADIPAAVLLSPGMEYAGIAAEPAVKGLSRPALLLAAKPDAYAYSSVLRLLSS
                     TGNNPNLRKMLLEQGGPQGAHGVQLFDGKLEKRILDWIRQQTAN"
     gene            complement(38205..>38532)
                     /locus_tag="P5079_00720"
     CDS             complement(38205..>38532)
                     /locus_tag="P5079_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY28538.1"
                     /translation="DEGSLTGAFTENTVFEDGDFRGDYFKGRLREVVARIDRLRPLLA
                     ESAPDMATAALKFCLSHPAVSTVIPGMRKPPHVEANVRASDLPPFSSDLLEKLKAHSW
                     PRNFYR"
BASE COUNT         7516 a        11051 c        12515 g         7450 t
ORIGIN      
        1 tacccggagc gactgcggcg ttacgaagaa ttttgccggc tcgccctcga attgggggcg
       61 ccggagacgt cgggcgtgtt gtccgtcaag ccggacaagt ggttcctttt gggggactat
      121 tacaggcaca gaaaacagtt cgtccgcgcg gcggcatgct atctgcggtc ttggcggagg
      181 gagtcgcgca acaaggaggt gctggtcaag gcggcggagt gctgccggga actcggcgat
      241 gcggcgcggg cgcggcgcta ttatgaaaag gccctcgcca tcaacgcttc ggttgccgga
      301 ttcggcgagg acccgtacga cgcggtcatc cggaaggcgt tggaatccct cgggccggcc
      361 gcgaaatctt cttccccgct ggtgagggcg tgagcacggg aaccgtttct ccaaaggtgg
      421 tcgacgccct gcgaaaactc gatgcggacc gtcgttccgc cgcggagcgg aggttcgtcc
      481 gcccggttct ggggtttctg gggaaactgc ggagaagata tctgccggaa ctggacgaaa
      541 atatcgtacg cctcctctgg gcccgccggg cctggcgcaa gttggcgaat tttcttctgg
      601 tggaattgca ggcggagaac atggggacgc gcgtgaccgg gaaaccctat tggctgacca
      661 tcgacccgac caatttctgc caattgcggt gcccgttctg ccccaccggc ggggaacgcc
      721 acatccgttc caaggcggtg ctgaaactgg aggatttcaa acggctcatg gacgagctgg
      781 ggccgacgct cctgcacgtg gatttcatga actgggggga acccctcctc aacagatata
      841 ttttcgagat ggtctcctac gcgaagaaat tcgacatcga cacgaaaatg gacaccaacc
      901 tcaacgagtt cagcgaggag atggcggaaa agatggtgcg gtccgggttg gactgcgtca
      961 gtctttccat cgacggcttg acgcaagaga cctattccaa ataccgcgtt cgcggggatt
     1021 tcgacaaggt gatgcggaac ctggaaacgc tcattcgaaa gaagaaagag ctgggaagtt
     1081 cccggccgta cgtcatatgg cagttcctgg tgttcaaaca caacgaacac gaagtggaac
     1141 gggtgaggca gctgggaaag gagatggggg tggatgtggt gggggtgacc ccggcgttcc
     1201 tcccgttccg gcccggcatc aaggacgagt ggctgccgct caaaccggaa catcgccatt
     1261 acgatcccga gacgttcccg gaccggcctc cctgggagtg ggagtcggcg ccgaaaacgg
     1321 aggccccgtc cctgcccgat ccggccccgg tcgcgcctct gccggaggag aaactatcgg
     1381 cggccgcggt tccgcacgtg gaggttctgc ccccgcgcgg cggggtccgg tcgataatag
     1441 aacggttctt cggccggaaa agggggggag cgggaaaaga agagaccccc gtcatcaacg
     1501 tgcaggtcta caaggaaccc gagaagcggc cccggtgcaa ctggccctgg gcgggcatcg
     1561 ccgtcaatcc gaacggttcc gtctccccgt gctgttcggt ggaggaagag atctacgatt
     1621 tcgggaacat tttcaccgag ggattttccg gaatatggaa cgggcccaac taccgtcgtg
     1681 cccggcgcca catccgggat tatgtgaaac atcggcagga cgtcaagacg cgttcggaac
     1741 acgcgtgtga gcgctgtttc agcatcggcc gggcgaattt cacgttccag ccctggtggc
     1801 ggttcagtca gttcgattgg tggtgggggc tgcgggagaa agacccccgt tgaaacggag
     1861 ggaaaggaaa ggtcatgcag gataacgcgt tgcttatgac ggacgaacac gttcagcgga
     1921 aattgaacat cgcgctgaac cggctcgaat tcgaacagaa gagggcggtg gtgtcgtcga
     1981 cgccggaggt gatcaccatc ggcgcgcaca accactgcaa tgcccattgc atctggtgtc
     2041 tggaggggga catcagcgtc ttcgacctcg accggtacaa ggaatttttc gagaagtccc
     2101 tcgggcacgt cattcggaag gcgcgccacg tgtcttttgt ggggttcggc gagctcctct
     2161 ggctgccgcg ggtggaacag ttcctcgacc atctcaacga gaccatcccg gacgtcgtca
     2221 agcagttcac caccaacggc accccgctgc ggcccgacaa gatcgtcgag aagcttctgg
     2281 tggggcggta ttccatccgg atctccctcc acgcctccac gccggaactg cacgcgaaga
     2341 tcacccgcat ccgagagttg gacaggatat tggagaatct gcggtatctg gtcaaattgc
     2401 gggagcattt ccagcggcga aaatatctcc acgtggagct tttcaacgtg ctgacgaagg
     2461 acaacatcga caacctgccg gacttcgtcc gtctggcggc ggagctcgat ctgccgcagg
     2521 tggtgtgcag ctacatgacc attttttcca aggagcacat cgagatgtcc ctcttcttcg
     2581 accaacagcg ggccaacgac cgcctggccg aggcggaaga ggtggcgaaa aaattgggcg
     2641 tcatggtcca gctcccgccg cgcttcggac agggggggcg cgaccgggag cggccggccg
     2701 gcgaatgcag cgacccgtgg caatattttt acgccgaaac acaggggtcc gtcctcccct
     2761 gttgctacgc gggcggccac gtgggttatc tcgataaaca atctttcgag gaattgtgga
     2821 acggcgagtt ctatcaaaac ctgcggaagg ggctggccgg cgagatgccc gcgcacgagt
     2881 ggtgcaagaa ctgcgtgaag ttcaaggggc attacaccgt caacaacgtg ctctgtcata
     2941 tcaccaaccg tcctgagacg caaaagatga tcgtggagga aatggagcgg cggggtttgc
     3001 tcaagccgac ggccgccgga ggagaggtct aatggaaaca acgccgccca ccagccttga
     3061 acgcccccaa cgccaggtcg ttttcgcctg ggacatgcac tacagctgca atttccgctg
     3121 cccttactgt ttctatacgc tgaccggatg gcatgagctg gccaagaaga gcacctataa
     3181 atccgccgag gaatggaagc gggtttggca ccgcctgtac gaaaaatacg gcagctgcca
     3241 catccgtatc accgccggag agccgttcac gtatcccggg ttcgtcgacg tcctcgccgc
     3301 cctttcgcag gaacattggg tgcaggtgac cagcaacctg tccctgcggc ccctgctgga
     3361 ggatttcgtc cggcgggcca acaaccggcg ggtggagttg gactgcacct tccatccctt
     3421 cgaggcgaaa ttcgaggact tccttcagaa cgtgcttttg ctgcgcggtc gcggcttcat
     3481 cgccaacgtg tgctatctgg cttacccgcc gcagatggcc cgcatggagg agttcaagag
     3541 gcgtttcaag gagaacggca tccacatgaa catggcggtg ttgtggggcc gctacgccgg
     3601 gaaggaatat ccgcacgcgt acactcccga agagaaacgt tggatcaaag aggtgatcgg
     3661 ctatgagatc gggccggaga ccgtcggcct ggaacccatc cccaccaaag ggaagatctg
     3721 cggcgccggc cagcgctacg cggtgattca tgccgacggc cgggccgtcc ggtgcggcca
     3781 gctgggagaa gagaccatcg gcaacatcct tgacgagaac ttcgaacttt ttcaaacggg
     3841 ccggccctgc ccgtccgact actgccgttg caaggagttc cagatggcgt gggaggagga
     3901 ggagaagcag gccttgctcc aacgaggaga ggtgaaggtt tgaggactgt ccgggagatg
     3961 gggatcgtat gaaaatcgct ttggtgcagt gccccgtctg gggggtcgct gatccgccgc
     4021 tggggccggc ccagttggcc ggctgcctca aagcggcggg gcacgaggtt cgcgtgttcg
     4081 atttgaacgt gactctctgg aggaaggcgc cgccccggca tgccaatctg tggctttggg
     4141 agatgtcccg gttctggaat tctccggaga tcgtcgaccg ttttttcgcg gacaaccgcc
     4201 gcctcatcga atcttcggtg caggcgatat tgcgttcggg ggcgcgggtc gtggctttct
     4261 ccgtcaacga cgggtcccag tgggcctccc tcaaggtgtc ccaatggatc aaggaggcag
     4321 acgcttcccg ggtcgtcgtc ttcggcggcc aatatttttt caaggggacc gcggcggagg
     4381 agattttaaa ggtttcggga gtggatgcgg tacttcgggg tccggcggaa gaatccttgc
     4441 ccgcgctggc gggcgatgtg gagacgttcg gccgaccgcg tcggcggccg ggcctccttt
     4501 tccgggagaa cggctcgttg gtcgacggcg gcccggcgtc cgtccccgct cttctggaca
     4561 agttgccttt cgccgatttc accgggtttc cgttgaaact ctacgccgaa cagggacgga
     4621 tcccgctgag cgcctcgcgg ggatgcgttt gggcgtgcca tttttgcagc gccggccgtt
     4681 tctggccgaa ctactcgtac atgagcgggg accggatctt tgcggaagtg aaacatcaga
     4741 ggtcgctttt cccctctcgc cggcacgtgg agttctacga catcaccgcc aacggccggc
     4801 cggaggccct gcgccggttt tccgagttga tgatcgagga gcggttggcc ggccgtcccg
     4861 ccggccacac cgggtggaag atcaacgcca tcgtccggcc ggagatgacg cccgacctcc
     4921 tgcgcaaaat gaaagccgcc ggatgcctct ccgtcatcta tgggatcgag agcggttcgc
     4981 cgggcgtttt gaaacggatg aacaaacatt actcccttcc gttggccgag cgggtgctgg
     5041 ccgacacgca cgcggcgggg attcacacca ccgccaattt tatggtgggt ttccccggag
     5101 aaacggagga ggattttcag cggacgctcg attttttgaa acggaacaag cgttccctcg
     5161 accgggccta tcccagcgcc accttcacca gcttggagga acacagtcct ctggcggaga
     5221 attacgccgc tttcgggatc gaaagcgaga acggccggcg accgcacaac ctctattgga
     5281 agatgccgga agggaacgac tatccggttc ggctggagcg gtacaaaaaa ttccgggcgc
     5341 tggcgctccg gctggggatc gaccgcgcca aggggctgca cggcgatctg gcgtcccatg
     5401 cggcgaggga cctcgccgcc tatcatcgat acgccggcgc cccgctccgc gccctggcgt
     5461 tgtaccgggc ctatttgcgg cgggaccccg ggaacggacc ggtgcggcat gaaatggagg
     5521 agttgttgat ggaaatttcc ggcaagaaag cgtccgccgc ggtgccgcgg ggctgtaaga
     5581 aacgacggac gacgtcccgt cccatcgcga ggacggtggc atgaaggtcg ccctggtaca
     5641 gatgccggtg tggtggaccc tcgaccctcc tctcgggttg gcccagatgg cgggatgtct
     5701 gcgcgccgcc ggccacgagg tctccgtttt cgatttgaac atacagcttt ggcgtcgacg
     5761 ctcgccgcaa agccatgctc tgtggtcgtg ggagcagttc catttctgga attacgagga
     5821 gcgggtggac aagttcttca aggagaaccg ggcggcgttg gaatcgttcc tcgagtcgat
     5881 ccttaagacc gacgcgcgcg tcatcggttt ttcggtcaac atcgggtcgc agatcccgtc
     5941 ccttcggatg gccgaaatga tcaagaaggc cgatccgtcc cggttggtgg ttttcggcgg
     6001 acagtatttt ttcctgggag acaagaccgc cgagatgttg aaaaaccctc cggtggacgt
     6061 ggtgtttcag ggcgccgccg acgaatcgtt cccgcttttt ctgacggact acgcccggac
     6121 cggccgggcc gtccttcggc cgggcgtggg cctgcggcag ggggagaaga tcgtcctggg
     6181 cggggccgtt tcccctgtcc cccagctcga caaggtcccg ttcgccgact tctcgctttt
     6241 ccccatggac ctctacgagg accaggggcg catcccgttc gccgcgtcgc gcggttgcgt
     6301 ctgggcgtgt cactattgca gcacgcatcg tttctggaac ggatacagtt gcatgagcgg
     6361 cgatcggatg ttcgctgaga tactccacca tcggcggttg ttcccgcaac ggacccacgt
     6421 ggatttttac gacatcaccg ccaacggccg gccggaaaac ctgtttcggt tcgcgcagct
     6481 ggtgcgcgac caccaccagc gttcgaccct gatgaccatc ggatggaaga tcaacgccat
     6541 cgtccggccc gagatgacgc ccgaactgtt gtccgcgctg tacgaggcgg ggtgcatgga
     6601 gatcatctac gggatcgaga gcggttctcc gcgggttttg aagcgcatga acaagaatta
     6661 cagcccgtcg gtggcggaac gcgtgttgcg cgacacgcac gccgcccgca tcaccaccac
     6721 cgcgaatttc atgctgggct atcccggcga gacggaggag gagttcggga tgaccctgga
     6781 tttccttcga gggaacgcgg cctccctcga caaggcttac gccagcgcca cgttcaccag
     6841 cttggaggaa aacagttacc tcaccgaaca tcgcgcggag ttcggcatcc gggacccggg
     6901 cctgaaggtg gggcattccc tttattggga aacggaggac ggcctgaact cctatttggt
     6961 gcggatggac cgttacgtgc gtttccgcgc cctggccgaa caactgggat tagaggccta
     7021 taaggggatc aacggcgagg tggagctgga acggctctcg aaactggccc agtatttttt
     7081 gtatacggac aatcgtctcc gggcaacgga gatgtacctt caatacgccc agagggccgc
     7141 cgacagcgag acggcgcggg gagaccttcg ggagttccag agggaacttg aagcgttccc
     7201 gcaggcgctc cggcggtttg aacgctggcg caacggtcat tcggaagtcc ccgcgtcggc
     7261 gcaggagacg atctgggaga aggcgaacgc ctggttgacc gacgacaaac cgtttcggct
     7321 ggaggagtgc cctcaattgg ccgaatggct tcgggagaac ggccgctaca aagactacgt
     7381 cgatgacatg ctggagaccc acgcgttttt gcagggattg aaatgccatg cttttctgcg
     7441 ctgggacaag gggttccggc ttttttgggg ccgggacgtc gtggaggggc ccggccgggt
     7501 cgcgggcctc cttgaagagg tttccgccgc gctgggacag gatgcctccg gcgggaaagt
     7561 gaaggacgaa cggatggtga acagccgatg aaagtggcgt tggttcaggc gcccgtgtgg
     7621 tggacgatcg acccgccgtt ggggttggcg cagatggccg gttgcgccat gcagtacggg
     7681 cacgacgtga gcgtgttcga cctgaacatc cttctctgga aggaccgtct gccgaaatat
     7741 cagaacatgt ggctgtggga acagtttcat ttctggaaca acccgcaggt ggtcgccgat
     7801 tttttcaacg acaaccgccg ggccatcgaa ggccacgtgc agtccatcct gcggacggac
     7861 gccaaggccg tttgtttttc ggtggcgctg ggcaatcagc tggccagcag catgatcgcc
     7921 cgcatggtca aaaaggaggc tcccaaaaaa tgggtcgtct tcgggggaca atattttttc
     7981 agggacaacc aaaaggcgaa ggaggtcatt ctcctcgata aaaacgtgga catcgtcgtc
     8041 cggggggcct ccgaccacct ttttccgaaa ctgttgagcg ttctggaaac gcggggcgag
     8101 ccgcgcccga tccggggtct gttgtataag caggggaggg tggtcacgga caccggattc
     8161 atcgaaccgc tccggcagct ggacagcgtc ccgttcgccg atttcaccaa gttccccatg
     8221 gaagtctacg aggaccaaaa ccgcatcccc atcgcggcgt cgcgcggctg cgtctgggcc
     8281 tgccggttct gcagctcctg caatttctgg ccggggtatt cctacatgag cggcgagcgc
     8341 atctatgcgg agatcctgtt ccatcgccgg cttttccccc agcggttcca tgtggagttc
     8401 tacgacatca ccgccaacgg ccgtccggag agcctttcga agctgtgcga cctgatcatc
     8461 gagcatgaat cgcgcaccgg ctggaaacat ttcaagtgga agatcaacgc catcatccgg
     8521 ccggagatga cgccggagtt gctcatgaaa atggccctgg ccaactgtca ggacatcatt
     8581 tacgggatcg aatcgggctc gccgcgcgtg ctcaagctga tgaataaaca ctacaacgtc
     8641 gacgtcgccg agcgggtact gcacgacacg cacgcggcgg ggatcaaggc ggtggggaat
     8701 ttcatgttcg ggttcccggg ggaaacggaa caggatttcc agatgactct ggattttctg
     8761 cgcaagaacc agaaatcttt cgaccgggtc tatgccagcg ccacgttcac cagcctggag
     8821 gagcacagct atctgacgga acatcaggag tccttcgggg tcgtcaaaag cgaagacaaa
     8881 cacaacagtc tttactggca gaacaaggac ggttcgaaca actacctcgt ccgcctggac
     8941 aggtacaaac ggttccggga tctggccatc gagttgggca tcgacgctta caaggggctg
     9001 gccggcagcg tcgagctgga tcatgtcctg aaccgggcca attattacga atacatggat
     9061 caccggtatc aggccgtgag cgcctactcc gactacctgg cgatggatcc catgaacgaa
     9121 cccatccggc accgtctgcg ggagttccag aaagaattgg cgggtctcgc gcgcgggttc
     9181 gagctgttgc ggcggtggga gcgggaacag ggagtttcga acccgctcgg ccgcgaggcg
     9241 tggcgggcgc tgtctccgca cctcctgaag gaacggccga tacgggcctt cgtcctcggc
     9301 cggaaggcgg gggccgccgt gcgaagttcc cggggcagga aatacctcaa ggcgttgctg
     9361 gcgttgcata acgcgctcca aaaactgaaa tgccgggcga cgctccggcg ttccgggagc
     9421 cgggtccggt tgttttacgg gacggacctt ttggagaacg aggaacaggt cgcgggcctg
     9481 acgaaaagag tggatcggat cgtcgtcgcc gcgccgggcg tttcggggaa ggggggcgtt
     9541 cccggatgag agtcgccgtc gttcaggccc ccgtggattg ggcggacgtt ccgcccagcc
     9601 ggctgtctca aatcgccggt tgcgccaggc agtacggctg cgaagtcgtc tggtgcgact
     9661 tgaacgcgat ttttcgaaaa cgggcgccgg agtccgcgcg gcccggctgg ggatggagtc
     9721 attttttgtt ttgggaaaac tcggacgttg tggcccggcg ctttcgggaa atgcgggatg
     9781 tggtccggga gcaggtcgaa cgcctgaagg aggcggacgt cgtctatttt tcagtgacga
     9841 aattttcact tcgggcgagc ctgctcttcg cgcggcggct gaaggaatcg gccccgaaag
     9901 gcctgatcgt tttcggaggg ccgttcttta ccgtcggcaa cctgaggatg cacgagttca
     9961 tcaccaatga gccggacgtg gacgtcgtgc tccaagggcc cggtgagttt ttattcgcga
    10021 aattgctccg cgatatgaag gtcttcggaa gaccgaatct tcagcccggc ctgcattacc
    10081 ggcacgggaa ggagctggtg atgggggatc ccgtcgaggg gttcggggac atggacgcgc
    10141 tgccgtttgc ggatttttcg ggccacgcgc cggaattata cgatgacggg gagagcattc
    10201 cgatccgctt gagccgcggt cagtccttcc ggtgccccgc gtgcggggcc gaggagcccc
    10261 gcctgcggcc ccgctggatg cgcggggagc ggctctacgc ggaggtcttg ttccaccgga
    10321 ggaatatgcc ccggcatacg aagcttttgt ttgaagacgt tgtcgacgag gagggggctc
    10381 gcacgttgga ttcgttttcg gagctcatgt gccaggacgc cgcgaaggcc ctgtctccgg
    10441 cggcgtgggg cgcggaattg gacctggccg cgaccgcggg gcggggatcg ttggcgacat
    10501 gggcgcgctc cgggtgccgc cgggtgacgg tgcggtttca ggaacgcccc ctcaccgacg
    10561 ctgaaaagcg ggccctcttg gaatttttcg aacacggccg cgcttgcggc atgaagatgg
    10621 tgccgaaact tttttttgcg gccccgggac aggagccgcg ggatgttcgg gaccaggccg
    10681 agttcgtcgc gggaaatgcg cggtttttcc aggacgggtt cgagatgggc tcgcttttct
    10741 acgatgcgct ggcggtggac gtccagcttc agcacgaaat ggaaaaaacg gcggtcggtc
    10801 ggtcggaccc gagcccctgt catttcggtt tcggagaccg atggacacgc tcgggcgatg
    10861 atttgaggtc ggacccagcc gcccgctgtc tggagctgaa gagcgtcctc cggcgggcgg
    10921 acctgtccct gctggaaaac cgtcgggaga cggtggagaa cgactacgcg ctgatgcgga
    10981 accaaatttg gcgatggcac ggtcatccga tacggcaatt ggaagggttg ttggcgtttt
    11041 ttgaaagaga gtcggaggac tcgatatacc accaggccgt gatcaaggag aatgcttttc
    11101 tggggaggat atgggagcgt ttgtcggcgg cggcccggct ggcgtcggcg gcgcaattct
    11161 acatcgcgaa ggcgttcaac agcccggatt gggacccgtc cgattgggag atcccggaag
    11221 acatcgggcg cgttttgcgg gaggggttgc ggcgggattg cctggaacgc cttcggagaa
    11281 gagggatccg gcggggaaac cgggtgcaga cgattcatcg ctacctgctc ctgattcgag
    11341 ccctccttgg ccgctcgaat gccctgggag acgtgaagtt cgacttcgat gtgccggcca
    11401 accggttccg gatcgtgtgg gagaacgacg acagcgacat ggcgtatgcc ggcgatctcg
    11461 gccggaagct tctctcgata ttgcgaaaga caaaaaaact cgccccggcg acacggcccg
    11521 gcgttccggc aatcctctct ccgaaagggg cgcccggtcg gcttaacgtg ccgggcgacc
    11581 attagcggcc cgggagatgc catgagcctg gcgttcgtat cgctgccgtt tcaggatacc
    11641 ggcctccccg acgtgcggct ggcccagttg atcggcatcg cgcgtcaaat ggggcaggac
    11701 gtttcggtcc acgacttcaa cgtgcgatgg cgccgccggc tgaggaaaac cgaaaaaaaa
    11761 ttctggctcc cggattcgcg cgtctgttgg ctggaaaaag tgctggcccg gcggttctgg
    11821 gaggatttga agggcgagtt tcaggcggac gtcgacggac tcctggacgg gaatccgtcg
    11881 cgcgtctttt tttggatcac cgcctccagc cttcagttga gcctgctcct ggccgcggag
    11941 atcaagaaaa gggctcgtcg ccccaaaatc attttcctca acccccattt gttcttgggg
    12001 gcgtttatgg acgaggatca ccgggcgatt tcggattttc tccagacgga gccggtcgac
    12061 ggaatcctcc aggggccgct ctacaacgat ttcgcccgga ttttgacgca gagggaggaa
    12121 ttgctgggga gcgcctccgg attccattgc gtcaaagacg gcatggtgca tctgggaaaa
    12181 aaacccaccg ttgaaaaagg cttcgacagc ctgccgtttt cggattattc ccttttcccg
    12241 cttcacctgt acgatgacaa agacgggttc ccgatgcagg tttcggacgg ttgcgcttgc
    12301 gacgcttgcc gtcgggccgg tttcgggccg gatgccatcc tcgcttccgg gatgcggatt
    12361 ttttccgaag cggcctacca ccggcggatg ctgcccggcc gccatcagat gaagatcgtc
    12421 cggccggcgg gagaaaaggt ttttccttcc ctgcaaaagt tcgccgagct cctttcggcc
    12481 gacatggaag cggtggggaa aaaatatttg gagtgggagt tcctgacgcg cgagccggag
    12541 aaaatgacgt cggaacagtt gggttggatg atcggggcgg gctgtaaaag gatcggcgtc
    12601 gttcatcgga cagggagggg gctccctccc tgtccgccgg agccgccgcg cggccggcgg
    12661 atcatcgccg aactgcatca ttacctcgtg gaagaggacg acgggacgcg ggacagtgtc
    12721 cccgccgaca tgggcccttc gggaggatat aagaagatta ttcggcatgt cgtcgaacag
    12781 gacggattgg acgcgcacta tttgcggaat ctctcccgga tggattacga attgaaatct
    12841 ggccgacccg gcggggacgg ggaattgccc caagagatct cctcgtgcgc gcgcgggccg
    12901 gaggggcggg aagaggggct gtcgcgctcg gcgttggcga tggcttcttt cctgaatgcg
    12961 cgcaaaatac tcttcgaccg gcgccatccg ttcgacgcgt tcgagttttt ccggtcgctc
    13021 tcgccggaac tccgcggtgg ccggctgatg cgtctttttt atcgccacct ggccaccgac
    13081 gcgcggagga tgatgtccat ggcggaccgc cttcaggcgg ccgaacagat tttcaatctg
    13141 ctgcctcagg agtcccgccg aaaggcgtgg atgggttccc cgacgttcca tcaggagggc
    13201 gatcacagca cctattgccg gcatcggatg gggatgaagg tattcaaaga ttacatgccc
    13261 caacgggggg aacgcctgct gatcgcggcc cttgcccggg cgacgcagat aagaatgttg
    13321 cgcgccgccg tcgtcttgga cgaggaccgc aacgaattcc tcatccgttg gggggaaagt
    13381 attcttccgg cgtcccgggt gcgccttctt tgccgggact tcctctcttt ggggctcggc
    13441 gttgccgccg agcgcgcgtc cgccggcccc tcgccggccg gagtcccggc gtgaaggtgg
    13501 ggctcgtcca gtcgccggtg tggtggacca tcgaccctcc gttggggctc gcccaggtgg
    13561 cgggatgtct gcgcgccgcc ggacacgagg tcgctgtgtg ggatttgaat attcttctct
    13621 ggaagaaccg gcttccgcag tatgagaaca tgtggctttg ggagcaatat cagttctgga
    13681 accaccccga catcgtcgac cggtttttcg aggataaccg ggagttcatc gacaagacgt
    13741 tggaggggat gctttccagc gactgccgcc tcttcgggtt ttcgatttat gccggcaccc
    13801 agcgggcggc cctccggttg gcgcagcgtc tcaaggaggc ggactcccgc cgccgggtcg
    13861 tgttcggcgg acagtatttc ttccgcgggc cgcatgccga ggagatgctc cggacgggtt
    13921 ttgtggacgc cgtggtgcgt ggggcggggg atgaactttt tcctcagttg gcggcggacc
    13981 tggaacggac cggcctgccc cggaaacgcg ccggccttct gttccgcgac ggggccgaca
    14041 tcgtcgacgg cggcccggcg gagtcgccga aaaatctgga cgccttgccg ttccctgatt
    14101 tttccgggtt cccaatggac ctctacacgg acaagacgcg catccccgtc gccgccagcc
    14161 gcggctgcgt gttggggtgc cgtttctgca gttcacggga attctggccg cattacagcg
    14221 gcatgagcgg cgaacggatc tttgcggaat tgaaacatca aaaggcgatg ttcccccaac
    14281 gggcgcactt tgagttctat gacctcaccg ccaacggccg gccggagaat ctgtaccggt
    14341 tcgccctgct ggccgccgat ttcatgatcc agcatcggcc ggagcggaat tttcgttgga
    14401 agataaacgc catcgttcgt ccggaaatga cccgcgacta tctggctgtc ctaaaggcgg
    14461 ggggatgcca ggatatcatc tatgggatcg agagcggctc cccggacgtt ttgtggcgga
    14521 tgaacaaaaa ttacgacccg gcggtggccg atcgggtgct ccaggccacc catgaggtgg
    14581 gcatccgcac cgtggccaac ttcatgttcg gttttcccgg agagaccgag gaggatttcc
    14641 aactgaccct cgatttcctc cgtcggaacc gcccctggct cgacatggcc tacggcagcg
    14701 ccacgttcac gagcctggaa gagtacagct atctggccga acatccgcgg gagttcgaca
    14761 tccgccagga gacggcccat acggcgcaca acctctactg ggacagcctt gacgggaaga
    14821 acacctatcc ggtccgcctc gaccgatacg cccggttccg gaggacgtgc cgtgaactcg
    14881 gcctggacgc ctataagggg ctggacggcg ccttggagct ggaccgcctt gccaatctgg
    14941 cgcactatta ccaataccgc gatcgacgcc tggaggcgat cggcgcctac gtggactatc
    15001 tggagcgcga ccgggccagc cgcccgatgc gcgagcaact cagacgatac cagggcgacc
    15061 tgcgtttgct ggtgaaggcc ctccgtctcg tggagagggc caaccgcttg ctcttgtccg
    15121 ccaacggcgc ccttcgggaa gggcaggaac ttttgcggag gacgctggag agcggagagc
    15181 ttttggcgat gcccggttct tcgcgaggcg agatcgaccg ggtccgcctt tatgtcgcgc
    15241 gcgcccagag attcctgcgg cggttgacgc acggcggcgg gttgcgttgg ggagaccggg
    15301 gactcgtcgt ctgttgggaa aaggaaccgg cgccgccgct ggaagagctg cgggcgctgc
    15361 ggtcgaaggt ggacaggctc atcgaacttt ccgagtcgga agaggcccgg acctcgcccg
    15421 cggcggttgt tccgggcgcg cgctgaggcc gcgaaaaaat gggggaaaca atgaacgttc
    15481 cgaacacgac ggtggagccg gggagtcccc gggacctccg tttgaaatta ttggccgaga
    15541 aggccgaaga caacatccgg cgcaacaacg aggaatatgc ccagcgccgg gtcgtcttgg
    15601 ccagcacgcc gcagaacatc ttcgtccaga tcaacgccgt ctgcaacgcc aactgcgttt
    15661 tttgttccaa gggatacgat tacccgcttt tccgcctgga cgattggctc aaacattacg
    15721 gcgacaagat cacgcccgcc ctggcgcagg cccggcaggt gatcctgacg ggctccgggg
    15781 aattcctcgg ccttccggac gcccagcgta tcttggaata tttcaattcg gagttcccgc
    15841 acgtcgaaaa atacgtggcc accaacgcca cctacctgac cccgaagatc gtcgacctca
    15901 tctgctcggg gtcgagcaaa tacgtcgtgc agctttccct gcacgccagc cgcccggaaa
    15961 tccagcgcgt gatgatgcgc tacggggcct atgaccgcgt gcagaagaac atccgggcgc
    16021 tgatggaggc ccgcgataag aagaacgtga acgtgctctt catgttcatc atgaccacgc
    16081 tcaacatcga cgacctgccg gatttcatcc gttacgcctc ccgcatgggg gccgacaagg
    16141 tcgtctgcgg atatttttat atctacgaat cgaaccagaa atacctgtcg ctgtatttca
    16201 tgcaggagaa ggccaaccag cggctggagg aggcccggcg ggtggcggac gaactgggga
    16261 tcaccctgca ggctccgccg aagttcggtt tgacgccgga agtctataaa gcgttcgacg
    16321 tctgccagga accgtggtac cagttcatga tgagcaacga cggctacgtg ctcccgtgcg
    16381 acgcctacgg gagtttcccc agggaagaaa acctgaacgt catgtcgttc tccgaactat
    16441 ggaacggccc gacctaccgg aaaataagga cggacatcgt cgacgggaaa ggatgtctcc
    16501 tcacctgttc ccggcataac cccaagagcg tcaacgattg gaattcccac gtcatccacc
    16561 ggttcaagga agacaggcag atcatcaagg aacaccatga atcgcttcga aggccgtaat
    16621 cgatggcaga gaccgagacc cgaaaaatga tgacagaccc accgtccgcg ccgctttatc
    16681 cgcggcggca tgccccgttt cgcgttcatt ggaactggta tctgctgcag cagtgcaatt
    16741 acgggtgcag ctattgtccg gaggtggagt ataagagaac gaaaaccgcc cccttgccga
    16801 tcctcaacgt gagcctggac gattggacgc ggatatggga tcgcctgttc gggctttacg
    16861 gatcggccct catccgtttc gccggcgggg aaccgttcat cttcccccat ttcatcgatt
    16921 ttttggccat gctgacggaa cacaacgcca tcgatgtgac caccaatctg agtttcgatg
    16981 tggacgagtt tctgaggaag gtgacgcctg acgcgctggc catttccgcc agctaccatc
    17041 cggaacacgt ctctttgggg gagttcctgg gaaaactgga aagactgaag cgcgaagggt
    17101 tctgcgtttc ggccgcgttg gtggcctatc cgacgttcct cggctcccta aaaaagacga
    17161 ttgagggttt tgaccgggcc gatatccagc tgaagctcat cccgttccgc gggaagtatc
    17221 aggggcgcga ctatccgctc gcctacacgc aggatgaaaa ggacgtcatg cggcttgcca
    17281 cgcagacccg gaacaccgac ggaaacgtca tcaacgcgca gtggttccgc gagatggtgg
    17341 aaaaagagga gaaacgccag gaggagatcc gggcctacaa cgagcgccgt cccgatccgt
    17401 ccccgccgcc ggcgcccgtt ccggagagcg ggaacggaaa gtcggccccg ccgctttgcc
    17461 gcatggggga gaattacgcc aagatctatg ccgacgggac ggtacgccgg tgctgttgtc
    17521 ccggcgtccc cgtcatgggc cgcatcatgg acaaagattt ccgattgctc gacgccgccg
    17581 gcccatgcga tgtcccctgt ctgtgctgga aacccatgaa gctcggggag tatgaatccc
    17641 ggtgggagac gctgtggcag ggactggtgc atcggaaaca tccgttaaaa accgaggggg
    17701 attagagggc gcgcacgatg acggacggaa cggcggcttc ccaaagcgga gtatcggaaa
    17761 acggcggcgc ggcgacgatg ccgtccgccg gaccgagact gtccgaattt catcgcacca
    17821 tgcccgtttt ttacaactgg gagatccatt attcctgcaa ttaccgttgt tcctattgcc
    17881 ccttcaccgt ttccggttgg catacctttt cctccaaaaa cgcctacccg cccctgtcgc
    17941 gtttggaatc ggcgtggcgg cggctttacg agcgttacgg caagggacat atcgcgatct
    18001 ccggcggcga accgtgcacg tatccggagt tcgtcccgct ggtgcagacg ttgacccgca
    18061 tgcattcggt ggagctcaac acgaacctga gcttcgacga ggagaagttc gcggccgcgg
    18121 tcgacctgaa acgcattcgg ttggccgcca gtttccatcc tcaattcgcc gcgttcgagg
    18181 attttttcaa gaggaccgtg tatttcaagg agcgcggggt ggatattttc gtgaattacg
    18241 tctcctttcc ggagcagctg gaggagatgt ggcggtataa aaaggagttc caggcggccg
    18301 gcgttccgtt ttacatcatg cccttcaacg gcaaatacga ggaacgcgtc tatccccagg
    18361 gcgccacgga acgggaaaag gcgtgttacg acgcggccat gaacggggtg gacccggaaa
    18421 cgtggatcaa tcgtcagcgg cgcgaatggc ccggccatcc ggggcagtat tttccaaacg
    18481 gggtgacgcc cagcgaggcc gcccggttgg aacggcaagg cgaaaaaagg gtggttcccc
    18541 cggtggtcgc gcccgaactt gcggccgccg cgaaacgggc cccggtcctt tgcctgatgg
    18601 gataccggta ctgcaagatc cgtccgaacg gggaaacgta tcgctgctgc gcctggttgg
    18661 ccggcaacac ggacctgccg aaacccatct acctgggaaa cctctacgag gatgaaaaat
    18721 tttctttatt ggaggagccg gcgatgtgcg attacacgcc ctgcccctgc gaacgatgca
    18781 tggtggtggg ggaggaggac cgatggagac atcgctgggt gacgacggag tgaggggacg
    18841 ttgagcatgg aaaaggccgc ccaaaacaga cacccccagg tgcggttcac ctggaacatc
    18901 cattggagct gcaattatcg ctgcagctac tgtttctttg agacgcactg ggaggaatat
    18961 gggaaacgga acgtctataa atccgtcgat gaatggatgg gttattggga aagggccgcc
    19021 gagctctacg gccgctgttt catcaccatc aacggcgggg agccgttcac gtatcccgga
    19081 ttcgtcgacc tcatcgcccg gctctcgcgg gtccattggc ccatcaacat caccaccaac
    19141 acctcgctgc atctcaaaga gttcgtgaaa aaagtggacc ccgaacgggt ttcgttgtcc
    19201 gtcagctatc accccgaata ccaccgggtg gacgatttcc tggaaaaggt ttttttcctg
    19261 cggcagtccg gttttgtggg ggggtgcgtc aattttgtcg cccacccgaa ccacatcgga
    19321 accctgccgg ccctgttgga aaaattcgtc ggcatcgggg agagcctgaa agtgaacccg
    19381 ttcatcgggg agcacgccgg ccggcggtac cccgacgggt acaccccgga acagaaggag
    19441 attttgggga tgcgcgattc gtggatggac gacaagcggc acaaggggat gctgtgccgg
    19501 gccgggaacc tctccggact gctcttgccc aacggcaacg tggcccgctg cggacagatc
    19561 ggcgaccggc acatcgtcgg cagttttttc gatccgaagt tccgtctctt gccggaaccg
    19621 cttccttgcg acgtggagtt ttgcccgtgc gacgagtgga aagtggtgcc ggacgaaaaa
    19681 gagccggaaa acgcgggggc gtggcttccc tgacgacggg ggaggcgatg ccgaaaggag
    19741 ggatatgcgc tcgcggttga gttttacgtg ggacctgctc tatcactgca actaccggtg
    19801 ttcctattgc tggtgggacc cgcattggct caagttcgcg gagaaatacg ccggataccc
    19861 gtcagcccga cagtggatcc gggcttggga gaacgtccga cgtctttccg gagaggccga
    19921 catctccgtt ttgggagggg aacccctgat gtacgcgggg ttttccgaca tggtgcgcgc
    19981 cctgacgacg gcgcaggggc atcatgtctg catcacgacg aatttataca ccggctcccg
    20041 gcagctggcg gatttcgtga aggggatttc gccagaaaag ctcgatctgt cgtgcagcta
    20101 ccatccgcat ttcgcctcgc tgacagaggt cctcgaaaaa gtgaaggccc tgaaggaact
    20161 gggtttccat gttcccgtct atctgatctc cttccccccg ttcctggagg agttgccgtc
    20221 gtacgaggag acggtgcggc gggccggcgg ggatttccga ttgcgtattt tctgcggcga
    20281 gtacaacggg aagacgtatc ctcgggatta cacggacccg gagcgggccc tcctttcaaa
    20341 gatgctcggc aatccggtgc aggtggacta ccaaatcgat cagaagacga cgttgggtga
    20401 acggtgctat tccggggcgg tctacggcaa cgtgaagccg accggcgacg tgtaccgttg
    20461 cggacaatcc gctatcgacg gaaaacccat gggcaacatc ttcgacgagg atttccacat
    20521 gttgccgggg ccgacgccgt gcccgttcac gcactgttcc tgtcaggaat acgaatacgt
    20581 ggccaaggaa tacgaacgga aacgcgccct ccgggccggg atgtcggtcg catgaggagc
    20641 tcccggggac gcggggtcct ttcggaacgc catgagtgat caagagaggg cggcccacgt
    20701 ccgggcgtgg ttcgaggggg agccggcggg tccgtttacc attcaattga accccacgat
    20761 ggcctgcaat ctgaattgtc ttttttgccg tcgacaaaac cagctggaag ccttctatcg
    20821 ggatcatcgg gaactcccgg accagaaata catggaaatc gtggaggagg ccgtggcgct
    20881 gggcgtgaaa gccatcaaca tcaagggggg cggcgaaccg ctgatccggc gggccctgat
    20941 gcgggagctc gttccgctga tccgccggaa caaggtgcac ggatcgctca tcaccaacgg
    21001 gaccctgttg gacgaaccgt tggcggagtt gatggtgcgg tgggagtgga acgagctttc
    21061 ggtgagtttg gactccccgg aggcggccac gcacgattat ttgcgggaca agccggggac
    21121 gttcgaccgg ttgatggccg gacagaggtg gatcaacgaa tttaaacgcc ggcataaggc
    21181 cgctttgccg cacgtcaagt ggcactccgt gttgacgaat cggaaccatc gccgggtggc
    21241 cgatatgctc cgcctggcgg cggagaacgg ggcggcggag gtggagttcg actcgctgga
    21301 tttgagcgaa ccgtcgtcgg ccgttttgaa actggacgaa ggggatgtaa gggagtttca
    21361 ggaggggctt cctccggtcc tggctctcgc cgagcgtttg gggatacgcc acaacctcca
    21421 gaatttcgat cgccgcgatt acgcggtgcg gggaggggcc aacggcgtca agaccggcga
    21481 taaaacatcg cccgcccccg gcggttcgcc gtatgagcgg atcccctgtt tcttcccctg
    21541 gttccactgc gacatcactc cggacggcag cctcgtgccg tgttgctacg gggaaggata
    21601 cgagtcccgg gaaaacctcc atcggatgtc tttccgggag gcctggcggg gacgggggat
    21661 gaaagaatac cgcgacagca tgcgggacgg gaccatgaag ccgttttgtc ggaattgcac
    21721 cgcctgttac cgggaccaga acgaccgcat ccgggaagct ctccggtgcg gcccggcggg
    21781 cgatgctccg cgccgggaga aagttttttg aagaacgaca tgttgttctt ccgaggttga
    21841 aggatgccgg gaatgtcgcc gaaccgtcct atggattact tctgggtcgt catgacccaa
    21901 tgtgattgcc ggtgccccta ctgtatctac ggtctgtacc cgttggaccg gaggaaggtg
    21961 ggctattttt ataccgcgga agaatggctg gccgggtggg agcggatgta taaaaaatac
    22021 ggcgaaggcc gcatcgtcct taccggcggg gaaccgaccc tttatcccgg ttttacggac
    22081 ctggcggtcc agctgagccg atggtaccag atcaacatcg acacgaattt ccaacttccc
    22141 tccgcggagc tggagctcct tctgaaaaga ctgtctcccc ggcgggtgga tttcagcacc
    22201 agtttccacc cgttcatggc caagacggac gaattcattg ccaagatcca tctgctcaaa
    22261 cgaaacgggt tcaactacct gtgccgtctc gtggcctttc cgccgctggt cgaaaaaatc
    22321 ccagcgtggc ggaagacgtt cggggacgaa gatatcattc ttttcgtcaa cccgttttcg
    22381 ggcgagtgga atggcaggaa atatcccgaa tcctacacgc cggcggaaaa ggagatcatc
    22441 ttcggccaga agaacagccg ggagagcgcc cgcatccatc ccgccgacaa ggaattcgtc
    22501 cggcaaatgt tgaacatgga atctcctcgc ggtcgaccat gccgtaccgg cagcgaacac
    22561 gtgcgggtgg aggacaacgg atgggtctat cgctgcgttt cctacgccgc catggaacgg
    22621 gaaccgctgg ggaatttcat caaggacgac atcgttttgt gggatcggcc gcgcgtctgc
    22681 gaggcggacg cgtgcgaatg ggaataccgc tatctcgtcg acgaaacgga ccgattcaag
    22741 agcgataagg tcctgcggga cgccacgacc cgcgggggcg tgtgaccatg ggacagggac
    22801 ccgaaaaccg aaccacgttc ggctgggacc tgacctacgt gtgcaattat cgctgcccgt
    22861 attgcaccgt ctgggaccaa cccacgccga tcaaagacat ggacgtggcc gcctggcgga
    22921 agatatggga ccggttctac gaactttacg gggaatgtta catctacatg tccggcgggg
    22981 agccgtccgc ccatcgctgt tttttcgagc tggtggacat gctgtcgaaa aaacacatcg
    23041 tggacatatg caccaacctg tcgtgggacg tcggacggct ggcggacgcc atccctcccg
    23101 gccgtctgcg catcgcggcc acctttcatc cgtcgcaggt cctttttcgg gaatatgtgg
    23161 agaaggcggt gcgcatcaag gagtatcttc cgttgaggcc ggaggggccc acggtctatt
    23221 atgtcgccta tccggcgcag atcccggaga tggaggaaca tgcccggctt ttccgggaga
    23281 gggggctcgc gctggtcccc ctgccgtacg tggggcatga gaacgccctg gggaattccg
    23341 cggacgaaaa agaaaaaatc cgggccatct cccccaacaa gggagacccc ctgaagaaat
    23401 tggagttcca gctgaaagag ctgtccccga agggccggtt atgccgcgcc ggtcagaaat
    23461 acgccatgat ccgcggcgac ggatccgtgg accggtgcag ccagtatcgg gcgcatcaga
    23521 tgggggacat ccgggacgcc aactttcaac tgtgggattc accccgtccc tgcacgctgg
    23581 cgtggtgtcc ctacgagtcg caatggctcg tccgagagga gacccgttcg tgaagggaag
    23641 acggagattt tggggcggcc gttttttgtt ggtggcggcc ggagggcttc tccccttgct
    23701 tgtggcggga atcgccgagt ggaatttgcg gcgcgacggg ttccagtttg agccctggcg
    23761 gtattatcgt cggttcggag ccaatttcga ggcggaggag atcgagtcct acgccaaaga
    23821 ccccgacctc ttctggcggt tccggccgtc gcaaaccctc cggacctggt gggtggagga
    23881 ggcgacgata aaccgatacg gcttcagggg agaagatttt ccggaaaaga aagaacccgg
    23941 ggttttgcgc gtgttgtgcg cgggcgattc cggcactttc ggctgggggg tcagagacaa
    24001 cgagtcgttt tccgtctatt tgcaggagtt cctggggcgg cgctgggggg aagggaaagt
    24061 ccaggtcgtc aacgcggggg ttcccggtta ttcttctcat caggggctcc atgtcctgaa
    24121 gaaatggggg ccccggcttc gtccggacat cgttattttt tcgttcggca ggaacgatca
    24181 taatcccacg gcctggctga ccgaccgcca aaggaaaaag atcccgcgcc cgctggtgga
    24241 actgcagaac gtgttgctgc ggacccggct gtatcagtgg ttctacatgc gcaccacgct
    24301 ctatatgtac atcgaacacc gggaaaaatt tctggggagc cggcgggaga cggtgcgggt
    24361 aacgttgtcg gaatatgagg aaaatctgcg tcgggccgcg gcgctttgtc gcgacggagg
    24421 ggctgacatc gtttttgttc cacggagtca gcagatggcc tatatcgacg tgatgaagcg
    24481 ggtggcaaag gagaccgggt cccgctatgt ggatttggaa aaattttcct ccgatcgagg
    24541 gatcaaggac tactcgttgg gggaacatcc gggctggccc gcctataaac gcctggcgga
    24601 gatcgtcggc gaggaagttg cgcgcttaaa gactccgtga cggagccccc gcatgaaacc
    24661 tgagatggcg gaagaactca taaagaaact gtatcgcgtg gggcccgcgg gcgtgttggt
    24721 cgacggaaag cgggtcttgt atttttcccc ggccggtctt ccggccggcg cgccgccggc
    24781 cgacgtgatc ttcatccccg gcgagtcgtc ccggcaccgc tcattccccg acatcaaaaa
    24841 aatcgcgacg gcccagacca tcgtcgttgg ccgtccgtcc catatcagcc gctttccctg
    24901 caatctgttg tcggtggaag cggaaaagaa aggggccgtg ctgggaatgc cgatcgtttc
    24961 ccgaaagaat ccggcggcgc cgggagaaac ggattttctg gtcacggtgg acgggctgcg
    25021 tctcgcttac gtggcctccc tttcgggagg atcgtccgct tttggggagg cggacgtcgt
    25081 tttttcgtcc gctcccgaaa aggaggtttc ttttcaacag gcgatttccg ccatgaaacg
    25141 aatgagggtt cggttctttg ttccgctggg cgattcccgc ctgccggacg cgttcgttca
    25201 aagactggag gcggagaaga taggggtatt gcagctgccg gggctgaacg gcaaaaaccg
    25261 gccggcgggg atatcgaaag aaaaaacaag ccgtccggcg ggcccctcga cgacagcggt
    25321 cgggacattg tatcgtccga aaacggcggc cgatcggatc acgttcacct ggaacataca
    25381 ccacagctgc aattaccggt gcccctattg tttcatggcg accgacccca tccacacgtt
    25441 tcgaaagaac agctatcccg cgctggacca gcttgtgggg gtgtgggaaa ggatgtatcg
    25501 cctttacggg agctgtgtcc tgaaaatttc cggcggggag ccgttcatct atccgcactt
    25561 cgtcgaactg ttggagggac tttccgccca tcacttcctg gatttctcct ccaacctctc
    25621 ttgggacgtc cgcgggctga tcggccggtt gcctccgcgc tccgtccaca tcgatccgag
    25681 ttttcatccg ggtttttata aggacattga ggaattcgct gaaaaatgtc tcctccttaa
    25741 aaaacaggat tccctcgggt ccgtgcatat cgtcgcctac cccccgtttc ttcaaaagat
    25801 gaggggatac aaggaggtcc tccaaaggaa aggactggac atcgttttct taccgttccg
    25861 ggggcgctat cgggacaaac aatatcccga atcctactcg ccggccgaaa aagaattgct
    25921 ccgggatctg gcccggtcga accgggacga aacgaaaaac ccgccggccg cctctgcccc
    25981 gcccgcggcc gccaagccgt tcattgagaa ggtcaacgaa caatatttct cctggttcgt
    26041 ggagaacaag aacgcgccgg ggccgcaggg ggggcgtctc tgttggcagg gggcgcgtta
    26101 tgggaagatc tattctaatt tggatgttta ccggtgctgc acccgggagg attcgccgga
    26161 actgtacgtg gggaatttgt tggaccccga gttccgtctc ttggacggtc ctcgtccctg
    26221 cccaatgacg gactgcgcct gctggaaggc gatgttgtgc gggcaggaga acacatggaa
    26281 aacgctttgg aacacggaga attttcccat gccggcgcgc ctgaagacat gaacggcgcc
    26341 ggggcttggg acaacgaaaa gatgtgggaa tacgggaaac ggggaatgct ccgggacatc
    26401 aaggactggt gcaccgtcga tggcgctctc gggcgccttg gcccgaaaca gttttcggag
    26461 ggtctcattg ccggtcaggg cgacgcggtg gaaaattgat tggaaagggc cttgggatga
    26521 attgcgacaa agcgaagaac atgaagtcgt gcaactgcac gtatccgtgc gcccgtaagg
    26581 ggagatgttg cgagtgcttg gagtatcacc ggcgttccgg cgaattgccg gcgtgttatt
    26641 ttacggccgc cgcggagcgc acgtacgacc gctccgtcga aaattatctc cgctcccgcg
    26701 gcgggtagga agggcggaac gataaatcat gctggcgaaa atctcaagcg cgacggttca
    26761 ggggatcgac gggtttctcg tgcacgtgga ggtggacctt tccgcggggc ttccgagttt
    26821 ttccaccgtg ggactgccgg acgccgcggt gcgcgaatcg aaagaccgcg tggtggccgc
    26881 cgttcgcaat tccggcttcg attttcccat ccggaagatc accgtcaacc tttctcccgc
    26941 cgacattaaa aaggaaggca ccgcgttcga tctgccgacg gccgtcggcg ttttggcggc
    27001 ccaggaggcc gtgccctcgg cgcggctgaa cggatacgtt ttggtggggg aactcgcgct
    27061 ggacggccag gtgcgcccgg tgcgcgggat gttgccgatc gcgttggcgg cccgggatgc
    27121 gaaatgcgcc ggagtgattt tcccggccaa caattgggac gaggcgtcgg tggtggacgg
    27181 cgttgatctg gtgccggtct ccacgctcac ggagacggtc cggtttttgc ggggggattg
    27241 gcgccccgcc ttgccgactc gtgtgccgtc ggccggggga ggcaacggtt tttgcggcgc
    27301 ggacttcgcc gacgtaaagg gccagatttt cgccaaacgc gcgctggagg tggcggcggc
    27361 cggggggcat aatgtgctca tggtcgggcc gcccggttcc ggaaagacga tgctggccaa
    27421 acggatgccc ggcatcctgc ccgccatggg ttttgaggaa tctctccaga ccagcaagat
    27481 ccattccgtg gccggcctgt tggggcgggg acatgcgctc ttgtcggcgc ggccgttccg
    27541 aagtccgcat cacaccatct cgaacgtggc gctggtcggc ggcggcaatt tcccccgtcc
    27601 cggggaagtc tcgctggcgc atcacggggt cctttttctg gacgaactgc cggagttcaa
    27661 ccggaacgtg ttggaggtcc tgcggcagcc gctggaggac ggttgcgtga cggtttctcg
    27721 ggcggccagt tcgctgacat tcccggcgcg gttcatgttg atcgccgcca tgaacccgtg
    27781 cccttgcgga tattacggac atccggaacg tcattgtgtg tgcaccccgt tccaggtgca
    27841 gaaataccgc gccaaggttt ccgggccgct gttggaccgc atcgacctgc acatcgaggt
    27901 cccggcgttg cgcgtttccg agttgacgga gggcgcgccg gcgggggaga gcacggaagt
    27961 gctccgggag cgagtgatgg cggcgcgcgc ccgtcagggc gcgcgcttgt ccgccgcggg
    28021 gttgttctgc aacgcccaga tgaacgcgcg gcaggtgaag gagttctgcg ccgtggacgc
    28081 cgcctgccgc gaactcctga agatggcggt gtcccggctg ggcctttcgg cccgctcgtt
    28141 cgaccggata ttgaaagtgg cccgcaccct ggccgatctg gccggggtgg aaaacatcgc
    28201 ccaggaacac gtggccgagg ccatcgggta ccggtctttc gaccgtccgc cggagtattg
    28261 accgtcgtgt ggcccccgcg tcggaacgag ttcctcgaaa ggaccaaagg cgtgatggcc
    28321 tttggcgggt tgttggtgtt cttcggtggg ctgggggtcg tcggccatca gttgtggtat
    28381 tggctgcaat tcgggaaatg gaaaaaggtc cccatttggt gggctttgga gaaaatagga
    28441 ccgccggaga cttcgctggg gggatggttg gctcacccga attcctggta tggcctgcac
    28501 aagacgctcg ccgtcttctc gaagacgaca ccgttgtccg tgacgcttct cgtcgtcggc
    28561 gggctgatct tcgttgtttt tatttcaagc atggattcga agaggttgga ataacgacat
    28621 tttttcgagg gatgacggca tgactttcga caggggcagt caaggaaata cattaaagcc
    28681 aagcctccct tctcttcgcc ggacagcgat gtccggtttt ttttgtttcg cgcatctcgt
    28741 tgtgtttgct gcgatgtccc cggattttga aaattcctgg aatttctaca aaaaaagggt
    28801 ggcccaaggg gtcagccacg aagagaagat atacatgctc gaaaaaatgg agaggtccta
    28861 tggggacaag gggatcgatc tgagcgatct gcggaaggaa aaatcgcggg tggtgcacga
    28921 cgttcggacg cagatatcgg cctcgtgggc gttctatctc cggcgggtaa aagagggggc
    28981 cagcaaccgc gagcggtcgt atatcctgga aaagatgcag aaaagatacg gcgaaagcgg
    29041 cgccaacgtg ggtttcgtga gggaagagat ggaacgggtg gaggccctgg agagagagaa
    29101 gaacgttttc ggagagactg tggatgcgga gtcggtcgcc gcccccgcgc cgccgccgga
    29161 gaagacaaaa acagtacgga ctcagttgag ggatgtcctt tttctgcaga tcgaatatct
    29221 cccggacgag gacgttctgg tgaggaacaa acaggacgct caggggatgt tgccgggcct
    29281 gctggccgcc gggtacgact ttgccgacgc ggggacccac gtggccgacg gtatcggctg
    29341 tcggctcggt ttgatgtggc cggtgatgaa atatttcgga tggggcggca gcggtgagta
    29401 tatcatcggg ccgaaggagg agacggcggt gaacggcgtg agccgcgtgg cgttggacgg
    29461 ccgtcttcac cggacgacgg aaacgaattt tttccggttc ctggcgcaag gggagttccg
    29521 ggtgccgctg tacgggagat tttccggaag attcggggcg ggggcgggat atgccacctg
    29581 caagttgagc gagaacaccg gcaactcggg gaccttcgtt tcggcgttgg gggcccccat
    29641 ccactccaag atttcggagc ggtgggacgg ctggacgtgg gaattctctc caggactggt
    29701 ttggcgggga gacaacgtcg tcttggaggc cgggatcgtc tccgtatttc ttccgacgat
    29761 gaaacagcgg gaggatctga acgagtttaa atggaacccg accgggataa gattacggtt
    29821 acagttttaa ggaaagattt cccgaagatt cagtgaggta aaaccggcgc gaccgcgttt
    29881 cggcgggacc agcctttcag ccccttctcg gggaccccga tctccgcccg ctccccgcgt
    29941 gtttccaaca acaacacctt ctgcgcctgg ggcaccacga agcccactcg atattcgtcc
    30001 ccggccccgt ccgcgcctcg aggcgcgggt ccaacacgat gccgtaattt tctcccggac
    30061 cgtttttgcg gcgaccgaaa ccgccgaggg ccagaaggcc gaggagggcg ccccgccgca
    30121 ggaggatttt caggaaactc aggagaggcc cccgtcgttt tccatggagg gcctgttccc
    30181 ggccttgttt ttccgcttcg acgtgggact gtgaggggat cggtccggtc gggcgattgc
    30241 caatcgtcct gtaaatattt agtatatctc tacggtcgga aaaaagtcaa tttttcagac
    30301 ggacgcccct tgacagaagg gcgtttttcc attataaaag ggtcgaatag gagggaacat
    30361 gagattttca cctttattta tcgcggtttt ttcaatagca gtgggtgtcg gcgcccagga
    30421 ttgggtggac acaaagtcca cagaatccga aacacctttg ccggccgggg aatcttccac
    30481 ggaagttccc tccgccccgg ccgacctgcc gggtgtccct ccggccacct cccccgcggt
    30541 ggaacccctc gggagctccc tgcggtcccc ggacgttccc agcggttcct atgaagtcca
    30601 aaaaggggac accctttggg atttggcgag ccgattttat cagaacccct tcgattggaa
    30661 acgcatttgg gaggggaaca gggaattgat ccagaacccc gatttaattt acccgtcgca
    30721 aaggttccat attccgggaa gggagatggc ccggcctctc ccgccggttc cgccgccggc
    30781 cgagcccgcg accgccgaag ttttaccgga agaacctgcc ccggccgagc ccgaacccga
    30841 acctgaggag aaacccgaac cggcgccgga gaaagtgccg gtcccgagtc cgtacggcgc
    30901 gaaaacgatg accagcaaca cgatgatggc cgatgcgaaa tggaaaggcg acggccagat
    30961 catttcggat gtggccaaaa aggccttgat cagccaatac gatatggtct acctcaatat
    31021 cggcagcgaa gggggggccg tcccgcgccg ccgcggcgac atttaccgga aggcccagaa
    31081 ggtccgggcc ccccagtccg gccgcccgct cggcgtggta taccgtcggg tgggggtgat
    31141 tgagatcact caggacgtgc agaagaagag ctcggcggcg gtcgtcgtca ccagccacga
    31201 accggttaaa atcggggaca tcattaagct ccgggattga ggcggtccgc cgccgtgggt
    31261 cgcgccctcg ccctcgctgt ttgttgcctg gccgccgttt tttggtggca taatttcgtt
    31321 tcgagcgggc gttgtctggc ccagctggac gcccatcccc atcggcgggg caccgccacg
    31381 gcgctgttct atttcgcgcg ctattacgag attttcaaca agaacgacgt ggcgctggaa
    31441 cattacaaac ggatcgtcga gcggtatccg cgctcccgct atgggatgga ggcgcaatac
    31501 ggcgtggccg cttcctacga aaagatgaag cggttccacg acgccatcgt ggaatatgaa
    31561 aaatttctgg aagactatcc ggacaacaaa tacacggtgt ccgtccgcaa caacgttgaa
    31621 atattgaagt ctcgctgaag tccgtcggcc cggcgcgccg ggccacgacg gcggcctgtc
    31681 cgccccgcag cagaaagtcc cggcgatata agtattttac taggacgaat gtttctgtcc
    31741 tcggggagag gtctttcccc ggagggaagg agccggtttg gtgagtttcg ttcggttggt
    31801 ccgcgagcag atgaaggaaa aagggctgtc catccgccaa atatgtcgtt cggcggacat
    31861 cgacccgtcg ttcttcgcca aagtcctccg gggggaacgc cggccgccgg acgatgaacc
    31921 ggtgctccgc cggctggccc aggcgctttc cgtcgatctt gtgcggctga tggtttccgt
    31981 cggccgcctc ccggagtcct ggcgggccca atgcgaacgg ccggacgtca tggccgccct
    32041 gaacggactt ttcagtccgg agagggcgct ttcccatccg cctcggcccg ccgccgagag
    32101 gacgaagaaa gtttcggtcc cgtcggccgt ccggaaagcc ccgccgccgg aacgggttcc
    32161 ggtgcgcctc cccgcctcgg cgttgtcgga ggatctgctg tgagcgtgaa tttggacgag
    32221 cgccgggcgc gcgtcttgtt caatctggcc gacaacatga cgccggtccg gtttcgaaaa
    32281 attttggaga ggttcggttc cgccttggcg gcctacgagg cggacgcgga ggagtgggac
    32341 gggctggagg acgtgtcgtc ggcgcaggcc cgcaaactcc accgccattg ccgcgaagcc
    32401 gcttcccgcc tgccggacga gttggctttg ctcgaaaagc atcgcgcccg cgccctcatt
    32461 gaaacggacg aggagtttcc cgtgggcgtc cgggccctgt ccgacgcccc catcgtcctt
    32521 tacgtgaagg gggattatcg tccggcggat tcgctcgccg tggcgatggt ggggacgcgc
    32581 cagtccacgc cgtacggccg ggcggcgtgt gaaaaactgg cgcgggaact ggcccaggcg
    32641 ggcgtcaccg tggtcagcgg tctggcgcgc gggatcgacg ccgtcgccca cgccacggtc
    32701 cttaaatttg acggacgcac catcggtgtt ctggggagcg gtctggcccg gttttatccg
    32761 cccgagaaca agccgttggc cacccggatg gtcgagcggg gggccgtgat gagcgaattt
    32821 ccgcttgcca ccccgccgga tcgggggaac tttccccggc ggaaccgcct catctccggg
    32881 ctggcgttgg cggttgtggt ggtggaggcc gatgagacga gcggctccct catcacggcg
    32941 cggctggcgg gagagcaggg caaggatgtt tttgccgtcc ccgggtccat cttttccaaa
    33001 tacagccgcg gcccgcaccg cctgttgaag caaggggcgc atgccgtgga aggggtcgag
    33061 gatattctgg aagccatcga ggttttcaag aacctctccc gtcccgtgaa gagacccgcg
    33121 cccgttcaaa cgaatttgac cgccgctgaa accgccctga tggagtgcat tcgcctcgac
    33181 cccgtcggga tggacgagct gtcggtaaaa gcgcacctgc ccgcgtcggc gctgtcatcg
    33241 gcgctgttgg ggctagaact gaaaggaatg atcaaggcgt tgccggggcc ggcctacgta
    33301 aggtcggaac attccctctc gtctccgcct gtctgacaac catggccaaa tcgcttctca
    33361 tcgttgagtc tccaacaaaa gaaaaaacca tcggaaaaat cctggggaag aacttcgtca
    33421 tcaagagctc ctacggacac gttcgggatc tgcccaccaa ggagttcggg gtggacgtgg
    33481 agaagggttt tgcgccgcac tacgtcgttt tgcccaaagc caagaagatc attcccgagc
    33541 tgacccgtct ggccaaggac gccgaaacgg tgtatctggc cacggactat gaccgggagg
    33601 gcgagtccat cgcctggcac ctcagcgaga tactgaaact ggggaagacc aaaggccggc
    33661 gcatcacctt ccatgagatc acgccctccg ccatcaagga ggccatgaaa catccgcggg
    33721 ccattgatca ggcgctggtc aacgcccagg tggcgcgccg cgtgctcgac cgggtggtgg
    33781 gttatcggct ttcgccgctt ctttggaaaa aagtgcgccg cggcctttcc gccggaaggg
    33841 tgcagtcggt ggcggtgcgc ctgctctgca tccgggagga ggagatcgcg cggttcgtgg
    33901 cggaggaata ctggacactc accgcttttt tggagaagtc cggccaagtt ttttcggcgg
    33961 cgctctacgg ggagggcggg aagaaattcg acaagttcgc cttccgccac cgcgagccgc
    34021 tggacgaggc cgtaaagaaa ctgaacggcg cggcgttcac ggtgtcttcc gtggagccgc
    34081 aggagcggcg ccgttccccc gccccgccct tcaccaccgc cagcctgcag caggacgcct
    34141 cccgccggct tcatttctcc acgtcccgca ccatgatcgt ggcgcaacag ctctacgaag
    34201 gcatcgagct gggggccgaa ggggcggtgg ggttgatcac ttacatgagg acggactccg
    34261 tccaggtggc caaggaggcc caggcggagg cgcggcagtg gatcgagaaa aaattcggga
    34321 aagagcacgt gccgcccaag ccgcgcgtct acaagacgaa gaacaaggtg gcgcaggagg
    34381 cccatgaggc gatccggccc accaaaccgt cgcggacgcc cgacgaggtc cgggactttt
    34441 tgaccgatga gcagttcaag ctctatcagc tcatttggca gaggttcatg gccggccaga
    34501 tggcggacgc cgtatacgac acgctcaccg tggacatcgg cgccgccgga tacgttttcc
    34561 gcgccgccgg ccggaccctc cggttcgccg ggttcctggc cgtctacggg gagtcggagg
    34621 acgagaagag ccaggaagag accccgccgt tgccgccttt ggccgccggc gacaacctgg
    34681 cgctgaagga actgaagccg gaacagcatt tcaccgaacc gccgccgcgc tacaacgagg
    34741 ccagcctcgt caagaccttg gaggaacacg gcatcgggcg cccgtccacc tacgccccca
    34801 tcatccagac catcctcgac cgcggctatg tgcgcctgga agaccggcgt ttctatccca
    34861 cggacctggg gaagatggtg gataaacagt tgacggacca tttcccggac gtcgtcgacg
    34921 tggggttcac cgccaagatc gaggacagcc tcgaccggat cgcctccgcc gaagaagact
    34981 ggaaaaaagt gatcaacgac ttctacgccc cgttcgtgaa ggacctggag cgggccgaga
    35041 agaaaatgga aagggtgcag atcaagcccg ttgaaacgga ggagaaatgt ccccgttgct
    35101 ccgagctcat ggtcatccgg gagaaccggt acggccgcta tctggcctgc cggaactatc
    35161 cgacctgccg caccaccatc gcgctcgatc gcgacggaaa aaagatattg ccggaaccca
    35221 cctccgagtt gtgccccaac tgcggcaaag cgatgctcat gcggacccgc ggccggctgc
    35281 ggttcctggc ctgcagcggt tatccggaat gccgcaccac gttttccgtg gacaaggacg
    35341 ggaacaagat catccggccg aagcccgaga tgaccgacca gaagtgcgaa aaatgcggga
    35401 aaccgatgct caaacgcgtg ggcaaacgcg ggccgttcct ggcctgctcc ggattcccaa
    35461 gatgccgcaa cattaaaaag atccccgctt gaccgcgcgt tttcgccgcg cgcggcggcc
    35521 gggcgcttat ggagcttaaa gaaggcattc aacgttttct ggtctatctg cggggggagc
    35581 gcaacgcttc ccgcgccacg ctccgggcct atgaggcgga tctctccgcc ttcgtctctt
    35641 tcgtggccgc ccgatacaag tccgtccggt tgctcgactg cgaccggtcc ctgttgcggt
    35701 cctacctggc ggagttgcag tcgaaggccg ccaaacggaa caccctcctt cgaaagcacg
    35761 cctccctgcg atcccttttc cgctttttgc agagggagaa ctacatcgcc caaaacccca
    35821 tgttggcgtt gaagacgccg aaacgggagc ggcgcattcc cgggttcttg agtgaaaagg
    35881 aggtctcctc cctcctggag atgctcccgc cgcggccgca ggcgctggcc acgctgcgcg
    35941 acagggccct cttggagttc ctgtactcca ccgggctgcg tgtggaggaa gccgccgcgc
    36001 tcaacgtgga ggacgtggac atgtgggggg cctcggcgcg ggccctggga aaaggcgcga
    36061 aggaacggat cgtccccgtc ggggaaacgg ccctgaggat tttacgggaa tacctgaaga
    36121 tgcggaagat agacccgctg gagagttccc gtgtcgcccc ggcccggccg cttttcacca
    36181 atccccgggg cgggcggctc agttcccgaa gcatccgcag cgtggtggac cgatgggcgc
    36241 gccacgcggg gctccgccag cacgtgcacc cgcacatgtt gaggcattct ttcgccacgc
    36301 acctgctgga ccacggatgc gacctgcgcg ccgttcagga gatgttggga cataaaaatc
    36361 tctcgaccac gcagatttac acgcacatga ccaaggaacg tcttcgtaaa atctacgaca
    36421 aggcccatcc ccgtgcctaa gggaccgttg catcaggaca agatcagcct cctcatggag
    36481 gaaaccggat gtgaccaaaa acaggccgag ttggccatga cgtcggtcgg ctacaatctg
    36541 gaaaaggcca tccgcaccat cggcacgctg ctgaagaaca tcgtggtcgt ccgggtcaag
    36601 ttccacgtcc ccagccagca tctctacggg ctggtgatcc tcctcgccga cgtccgccgt
    36661 cgcagtttgt tccgtatccg agccgtcgtt tcctacaacc ccgcccttta tgagaccccg
    36721 ttggcgggcg actggtatga tttcgaaaag accatctacg ccttccgcct gcacgacggc
    36781 acgctgcagc aggtgaccca aaatctggag aaaacgctgc aggagtggtt cgaaaagaac
    36841 tcccaggagg ggttgtacga cgtcctgacc gtcgagcgga tcgaggggtt gcgggagatt
    36901 ttccaaaaat cgcttttcgg gtatttcacc gacaacgccc tcgagatgca actgtccagc
    36961 gacgaactca atctggagca gttccggcgc ttcaagctgg aagaggaaac cgtaccgtcc
    37021 cggccggagg gggggccgga cgaccattcg gggagcaagt tggtcttgaa catcgatctg
    37081 cagcaggacg cggacggcat ccccgcgcgc gaggtgaagg cgggagattt cgtctatgcc
    37141 ctgttgaccg acggccgcga catcgcgcag tacctctcga aattactggg cggcagcggg
    37201 gagcacgggc tcaagccgct gcccgcgccg gtggaatcct cccggcgcga cggtgagacc
    37261 atcagttttc agatacgtct gtcggggacg atcgtgggcg tggcggaggt cggggcggac
    37321 agcctggtgc ggttgcagaa gcggacggcg atgtcgtggt ggaggcggct ggtccctttt
    37381 tgggaataga aaagaggact gggcgcggag tcgccgcggt cagtttgcgg tctgttgtcg
    37441 gatccagtcc agaatacgtt tttccaattt cccgtcgaaa agctgaaccc cgtgagctcc
    37501 ctggggcccc ccctgttcaa gaagcatctt ccttaagttc gggttgttgc ccgtcgaaga
    37561 caagaggcgc aggacgctcg aataggcgta ggcgtccggt ttggcggcca gcagcagggc
    37621 cggccgggac aatcctttga cggccggctc ggcggcgatg ccggcgtatt ccatccccgg
    37681 ggagagcagt acggcggcgg ggatgtcggc ttgcaaggcc gcgacgttca gggagacgtt
    37741 ggcgcccagg ctggcgccga cgaggatcat gcgttttttt gagagttttt tttgttcatt
    37801 aagggcggtg acggcatcgg tcaggtccga gaccatcttg cgccagaaga gggggttttg
    37861 gccgaaggac gggtcgcggt aatccaccgt tgatccgcgg cgggtcaacc ggctggcgcc
    37921 gtggccccgg gcgtcgtagg caaaggttcc ccagccgcgt ccggccgcct gggccacgag
    37981 gggggcccat tcgccccggg tgctccccaa gccgtgcaag aggatgagga aaggggcctt
    38041 gtcggtttgg ggcggttcga aatcgccgac gatcagaacg ggatcgtcgc cgtccgacga
    38101 tgtgaggtaa cggaagattt ctccctgggc gggggtcgtc cccgccagac ccaggaggag
    38161 cgcgaggacg attttcgcgg ccgaaaggga gttcggcgtt ctggtcaccg gtaaaaattc
    38221 ctcggccagg agtgggcttt gagtttttcc aggaggtcgg aagagaacgg aggaaggtcg
    38281 ctcgcccgga cgttggcttc cacgtgcgga ggttttcgca tcccgggaat gacggtgctc
    38341 accgccggat ggctgagaca gaatttgagg gccgccgttg ccatgtcggg cgccgactcg
    38401 gcgaggaggg ggcggagacg gtcgatccgt gccacgacct ccctcagccg gcctttgaag
    38461 tagtcgccgc ggaaatcccc gtcctcgaag acggtgtttt ccgtgaacgc gccggtgagg
    38521 gatccctcgt cg
//
LOCUS       DAOTFD010000005        38465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_710_length_38465_cov_4.909242, whole genome shotgun sequence.
ACCESSION   DAOTFD010000005 DAOTFD010000000
VERSION     DAOTFD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 38465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38465
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_38465_cov_4.909242"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..970)
                     /locus_tag="P5079_00725"
     CDS             complement(86..970)
                     /locus_tag="P5079_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY28539.1"
                     /translation="MLKRASVLLVAACFLFAALGAAIKVASEALPSEEIVFFRNAFGL
                     LALMPLVLRNGFGSLKTKYILQHLYRGVFGLAAMYCSFYAIGRMRLADALLLSYTSPL
                     FMPFFAHFSLGEKIPSGLAWFLSLGFLGVLLILKPGLGLFQPVACLALASGVLAAMAL
                     VGIRRLTLTEPTVRIVFYFSLFSTLISAAPLAGRWVSPSAPLWLFLAGMGACASVGQL
                     CLTRAYGLAPASQIGPFIYATVVFAGLWDWFLWGQLPDGLGVLGAACVVAAGVLIIYK
                     IGDAPRSTESVSSRAGTA"
     gene            complement(1020..2183)
                     /locus_tag="P5079_00730"
     CDS             complement(1020..2183)
                     /locus_tag="P5079_00730"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRY28540.1"
                     /translation="MKAKISQRLESLPPYLFVAIDEKKKAALARGADIISLGVGDPDL
                     PTPPHIVAAGQKALAVPANHQYPFGAGLKTFRHAVAQWYKNRFNVVLDPDTEIHALIG
                     SKDGLTHFPLAFVNPGDAVLIPEPAYPAYNSAVLLAGGVPHFIPLLEKNDFLPQLEAI
                     PKEVLRKAKILYLNYPSNPLSAVAGRGFFEKVVAWARGYDFIVAHDAAYSEIYFGEPP
                     VSFLSIPGAKEIGVEFHSCSKTYNMTGWRAAWVCGNAEVVKALGRLKDNYDSGVFQAV
                     QEAAVAALSGPQDCVAEMRRIYKERRDVLVGGLRRLGWKVFEPPATFYVWVHVPEGYS
                     SAKTVERLLDEAHIVATPGNGLGPSGEGYVRLALTVPVPRLREALSRIEKIKW"
     gene            complement(2223..2621)
                     /locus_tag="P5079_00735"
     CDS             complement(2223..2621)
                     /locus_tag="P5079_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28541.1"
                     /translation="MRASPSWPARVRRYVAWLFGEDVSPDDVVYDPVHVAIVLVVVPA
                     VSGILYWLLWSLLVCEGGIFVKIVPLFQVLLTSKTWSDFGYEGYPYKLGIFEGWVVNV
                     TALALLMLALAVLWKIFEKNDGADGKTPPF"
     gene            complement(2623..3375)
                     /locus_tag="P5079_00740"
     CDS             complement(2623..3375)
                     /locus_tag="P5079_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY28542.1"
                     /translation="MIFCRFQTGRAVRWGRVSRGTVEELLPDPFGDSSPTGLVWPLKD
                     VQLLAPCEPTKIIAIGLNYADHAREFGHKIPKEPLIFLKPASAVIGPDEPIRLPAASR
                     QVEYEAELAVVIGRRARNVPLEGTKKYILGYTLMNDVTARDLQRSDGQWTRAKGFDTF
                     APLGPWIVSGISPEDLKIEAYVNGERRQSSRTSQLIFGVSRLVSHISQVMTLEPGDII
                     STGTPSGVGPLKRGDRVEIRVEKIGSLLNRVV"
     gene            complement(3389..4183)
                     /gene="dapB"
                     /locus_tag="P5079_00745"
     CDS             complement(3389..4183)
                     /gene="dapB"
                     /locus_tag="P5079_00745"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRY28543.1"
                     /translation="MIRLAICGVGGRVGAAILARAAGDKGVSLAGLVESKGHRLVGKE
                     LEGVVVSSDLGKVIEKCDAVIDFTSPESTLKNAALAAAAKKAMVVGTTGLRGTQKDEF
                     LKTIKPVPVVFSPNMSFGANLLFDVAESLAKLLPDYDVEITEVHHKLKQDAPSGTAQR
                     LAEAIARGRRPGSAFVYGRQGQVGARRSAEIGVHALRGGDVVGDHTVFFFGNGERLEL
                     IHRITTREAFATGAVAAAHWVVGRKPGLYDMRDVLGLSDRKQKAGK"
     gene            complement(4210..5106)
                     /gene="dapA"
                     /locus_tag="P5079_00750"
     CDS             complement(4210..5106)
                     /gene="dapA"
                     /locus_tag="P5079_00750"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRY28544.1"
                     /translation="MFEGSCVALVTPFKNGVVDEKKLAELVEFQISGGTSTIVPCGTT
                     GESATMSHKEHGRVIDLVVKFARKRCLVMAGTGSNSTTEAIALTKHAQEAGADGALLV
                     VPYYNKPTPRGLIEHFRAVARAVSLPQVLYNIPGRTGLNMLPATVIELARSEKNIVGI
                     KEASGGTDQAREIVQALGPKFDVLSGDDALTPAMYGVGGKGVVSVIANIIPKDVVALC
                     EALKKGDTQKAQELHARMTPLTKALFLETNPIPVKTAVEWLGLCSGELRLPLCPMEET
                     AKQKLVKAMKAYGLSEAPSVRG"
     gene            complement(5189..6040)
                     /gene="dapF"
                     /locus_tag="P5079_00755"
     CDS             complement(5189..6040)
                     /gene="dapF"
                     /locus_tag="P5079_00755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRY28545.1"
                     /translation="MAGEVSFTKMHGTGNDFIVLDGMRRSVRMTPALARRLCDRHFGV
                     GADQILILLPSATADLRMRIVNADGSEVEMCGNGIRCLARYARDNGHVRRDDMTVETL
                     AGVVRPVLRKDGGVRVDMGAPILEAARIPTRARGRVIERPLASLRNLKLRLPRSPLFF
                     TCVSMGNPHAVIFMKGVEDLPADVWGPALETHPFFPRRTNVEFVEVTGPETARVRVWE
                     RGAGLTMACGTGACAVTVAGVLTGRLRRAAALTLPGGVLRVEWAPDDHVYLTGPAEYV
                     FRGRFFL"
     gene            complement(6063..7331)
                     /gene="lysA"
                     /locus_tag="P5079_00760"
     CDS             complement(6063..7331)
                     /gene="lysA"
                     /locus_tag="P5079_00760"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRY28546.1"
                     /translation="MKTGYGTYHYDGGELWVEGVPLRTLAERLGTPLYVYSASKIRTQ
                     YRNFDRVFAGRSRTVCYAMKANSNLAVLRLLAEEGAGADIVSVGELVRARRAGIPPHR
                     IVFSGVGKTETEIVAAIRAGILMFNVESAEELEAIEAAARRLRRPAPVSLRVNPNVSV
                     DTHHHIATGKAENKFGVPYEKAVELYRYAARKPWLNIVGIQAHIGSQILEVRPYLETA
                     KKLLSLVDRLQSHGIYLHVLDLGGGLGVPYHGEKPPAPADLARRLLPMMKGRNLRLVF
                     EPGRFLVAESGCLLTRVLYRKETAHKNFVVVDAAMNDLARPALYDAYHPVWPVRRSSI
                     RGVATDVVGPVCESGDYLAKGRVLPRPHAGDFLAVLGAGAYGFSMSSQYNSRPRAAEV
                     LVFGSQWWVVRERETLNDLVRGEKIPPALK"
     gene            complement(7334..8341)
                     /locus_tag="P5079_00765"
     CDS             complement(7334..8341)
                     /locus_tag="P5079_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28547.1"
                     /translation="MRTPVRRWKNVALALLLGGAAWGRVCGGEGPSRKARWHWAAEAE
                     ELRSGKKYEPAAALLEKAVVPGAAEKDLVRWWPALGHCYEALKIYDRALDCYRQALQL
                     RPGNPDRMLDLARIYTLVDLHPQAVEIYEKILKKNRHRKDVLLALASSYCSAGRLDSA
                     RSTAERYLKAEPQDWGAHRLLAVVEESLGDTGAAARRWEKILAVQPAAAEYGYLGRLW
                     ARVDEYALAQRAFDKAESLGLKSPRIFVERGILAWRQGKNDEAARHWRRTLGLWPDDA
                     LARFLLALTEFEAGRYSPAKEEMKTASVKTTDGYMRGLMDDFLAALEKKAERGAAFKK
                     E"
     gene            complement(8338..9372)
                     /locus_tag="P5079_00770"
     CDS             complement(8338..9372)
                     /locus_tag="P5079_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY28548.1"
                     /translation="MNPISIGRGRGAAAVLSAVLLFLPGPVLAAPSASALDVLSDTSL
                     AAQEDQLTDALWAQFRRLPVERRPRVALVLGGGGARGLAHIGVLKVLTREGVPVDLVV
                     GTSVGALVGALYAAGLPVEEIERMGQEVGWDKISDMTAARVWRLFVSEELLRTEKMEH
                     YIGRYVGNKQFADLGKPFACVATDLKTGEQIVLREGSVALAARASATMPGLFQPVPYR
                     HRLLVDGGIVDNVPTDVAKMLGADVIVCVAVPANFSKYNVSNILMTLTQALYIQGEVI
                     SQERLKQADVLIAPKVEDVSAMELWRSQECIAAGEVAARESLPRLRRVLVKKFFERWL
                     ETPAEPRKTP"
     gene            complement(9410..10714)
                     /locus_tag="P5079_00775"
     CDS             complement(9410..10714)
                     /locus_tag="P5079_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28549.1"
                     /translation="MTRKLILSLSFLGACSLCLPRPAVCQEPSPEQVLTLPQGIERAL
                     QNNQRLLMAQEDIRISEQRLREAQSQYFPQLGLNFNSARYLAEQNYVMPPEFGSVLLR
                     PSRGLEPDMFYSARAWLKQPLYSGGRTRNTIRLSRTNLERARIQYEEIRSRLVFDVTK
                     AFYDVLLCQRKIQIRVEALNSLSALSLSVSSEDVRRRTELGVAQSRLRREIAERRREL
                     ESARLTFLSVLGVELYTQVGIQGELTALSFDMDLAKLLAWAQESRSEIRQTDFQQEMD
                     KLAVNLSQAERFPVVAFGAAYELNDPEFPLKTTQWSAALNVSLPLFDGFSSRARIRQK
                     RMQANQSRIRRAQIEDEVNLEVRESYADVVYWQNERLRREKELQEAESLTRGLENGKD
                     AGDRLRAKTWLIELRESYWEAVHGQRVAAAKLQKAVGRPLGE"
     gene            complement(10734..12098)
                     /gene="argH"
                     /locus_tag="P5079_00780"
     CDS             complement(10734..12098)
                     /gene="argH"
                     /locus_tag="P5079_00780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRY28550.1"
                     /translation="MKKNNRQTTATVRPEDFVASIGFDHRLAVYDLTASLAHVEMLGR
                     QRIISGRDRGRLVRGLAALLGRVKRGVRLPAAEDIHYAIEKALFKAAGPAAGRMHTAR
                     SRNDQTVTAFRLYVRDQIDVLSGSLEDLSAAFVQLAEKNRRAVMPGYTHLQPGQPILA
                     AHHFLAYAWMFQRDRERLADVRRRTNVLPLGAAALAGTTFPIDREWVARRLGFSGVIE
                     NSIDAVSDRDFLAEFLAALSLVMAHLSRFCEELMIWSNPCFAFVTVADPFVTGSSIMP
                     QKRNPDMAELVRGKTGRVYGSLTAVLTLLKAQPLAYNRDLQEDKPPVFDAVDAVKASL
                     DVTAPMVRSLRINADKMRAACRLGYILATDLADALVRRGMPFRQAHGVVAAAVNYAQE
                     RDLPLEDIPLPVLRKFSPLFGTWVHEVLSVDGAVAARKSRGGTAPAEVSRQIQRLRRL
                     LRKK"
     gene            complement(12155..13360)
                     /locus_tag="P5079_00785"
     CDS             complement(12155..13360)
                     /locus_tag="P5079_00785"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY28551.1"
                     /translation="MRKFNRVVVAYSGGLDTSVMLKWIRERYRCDVVACCVDLGQAED
                     YGRLRRRALTTGASRCFVVDARREFVEKYIWKVVQARALYEGRYLLGTALARPLIAEK
                     VAEVARRVKADGVAHGATGKGNDQVRFELTFKALAPELGILAPWREWEFKGREDEVAY
                     AQRHNIPVTVTKAKPYSSDANMWHISYEGGVLEDPNHPYKEDMFELTVSPEKAPARGE
                     TVEIEFVKGVPVSVNGRRRGPVEMIDLLNKIGGRNAVGRVDMVENRLVGIKSRGVYEA
                     PAATLLYAAHRELESLVLDRDTFHAKEGVGAKYGELVYYGLWPSLHRRALEAFVTETQ
                     KYMTGRVRLHLCKGNIVVKGRVSPYSLYWQKLVTFEREEIYNQRDAEGFINLFGLPLK
                     VKALLRKNR"
     gene            complement(13622..14836)
                     /locus_tag="P5079_00790"
     CDS             complement(13622..14836)
                     /locus_tag="P5079_00790"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRY28552.1"
                     /translation="MNKESHQFNTYKRQPVTFVRGHGPNLWDDKGRRYLDFFSGIAVC
                     GLGHADRDVARAVTKQVNILLHTSNLYYTRPQEDLARELSRRTFGGKVFFANSGAEAN
                     ECAIKLARRHGCRTPDRGRPRYQIIVFRNSFHGRTLATLAATAQKHPKKDFGPLPGGF
                     PAAEFGDLDSVRRKITPRTCAVLIEPIQGEGGVRTAPPAFFRGLRELCRKHHLLLMFD
                     EIQTGMGRTGTLFAYQNKEVVGPGIVPDVLTVAKGLANGLPIGATLAQKKTAALFGPG
                     DHGSTFGGGAVPCGAGLAVMKALNAGTLARVRRLGRLFREEFEKWQEEMSFIKEVRGA
                     GCILGLELRIPGGDVVAFCRERGLLVNCTAERVIRLLPPFCLTDAEAAHGLRILKEGL
                     RSLKNSSKRRAK"
     gene            complement(14833..15585)
                     /gene="argB"
                     /locus_tag="P5079_00795"
     CDS             complement(14833..15585)
                     /gene="argB"
                     /locus_tag="P5079_00795"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRY28553.1"
                     /translation="MTRSVWVVKFGGSLLSDDKARKAFLRQVARLARRRPVALVHGGG
                     PEISAALQKMGVKPVFVKGRRVTDDAAMTVVEGVLSGQVNKKLVGELCSLGVRAVGLS
                     GRDGGLLLARPVKGLGRVGEPLRAAPGILRTLLQGKFLPVISSVASDARGRALNVNAD
                     EAAAAVAVWLKADRLVYLTDVAGVLDARKKTIPKIHTRDIQGLIDRGVVTGGMIPKVL
                     SCRRAIRKGVREIDIIDGRAGLLKMKGTRLLP"
     gene            complement(15671..16243)
                     /gene="rsmD"
                     /locus_tag="P5079_00800"
     CDS             complement(15671..16243)
                     /gene="rsmD"
                     /locus_tag="P5079_00800"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRY28554.1"
                     /translation="MLRIQSGIARGRLLVGLHAGVDVRPILARIRKSLFDILRPRLAG
                     SFFLDLFAGTGAVGLEALSNGAARAVFVDGNRRCCRAIEGNAARLGFADQAEVHCGDI
                     TKSLAWLKGRPFDLVFLGPPYKDAEKSPLALTIATLMRVEEAGLLAPGGWVIAQHHKK
                     EPVDDVSGKWEMFRRNYYGDSVLSFFQFKG"
     gene            complement(16247..16675)
                     /locus_tag="P5079_00805"
     CDS             complement(16247..16675)
                     /locus_tag="P5079_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY28555.1"
                     /translation="MTQIKTHTTDLSLTTKGETDIVDITEPVEEAVRSSGVREGQALV
                     FAAGSTVGITAVEYESGVLRDLREAFEKLAPRRGRYHHEDAWHDGNGYAHVRSGLLGQ
                     SRVFPVRSGKLVRGTWQQIVLVDFDNRPRRRAVTVQVLGE"
     gene            complement(16793..18484)
                     /locus_tag="P5079_00810"
     CDS             complement(16793..18484)
                     /locus_tag="P5079_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28556.1"
                     /translation="MSAPKDILLIHPPFVRDSWEFPLAVPMLKSYLERRSVSVDALDL
                     NGRYVEWLLSPAAGQWRRRSTEAMRKTADDPLKMSALRHYLQLSETSRSQWEDWRGES
                     VRQDLLDFLFQAMHAPLSFPEHLGDDLGEYVARHGLMPFGMDDFLRTVMPPLQRETDY
                     KIIGFSITLPSQWGAAIAIVRMLRAQKTDAFLVLGGATMNTMTPEAQEKIMRMVPVDG
                     IVRFAGEKPLLELVRILKSRGDLKCVPALIYRSGKNIVGNDPRPVEPAEPDPPPDFSD
                     FSVSPLYSEDRYVPLLYARGCSWGRCVFCSYPRQWAGAYFRPVGDVVRDLKHHHDRYG
                     QRKFKLMGDEMPPRYAKDLAEALREHSMGVQWWTFARSSRGWTAELLEKVAASGGAEI
                     ALGVESFDGRVLKTMNKGYSAETAERNIGEMGELGIKTSVDLIWDFPTTTYEEAKHTW
                     SVAKRLAPLVRAYNCFTFGLEPHSEMGRHPERFGIEFVTIKKFLAHQACRTSQEFLGG
                     LGIFLSENTGGVIPTAVPGLVLFPEIRYWVDKRGMTPVEKREMASLWMELAVSKG"
     gene            complement(18556..18628)
                     /locus_tag="P5079_00815"
     tRNA            complement(18556..18628)
                     /locus_tag="P5079_00815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18593..18595),aa:Ala,seq:ggc)
     gene            complement(18804..20762)
                     /locus_tag="P5079_00820"
     CDS             complement(18804..20762)
                     /locus_tag="P5079_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28557.1"
                     /translation="MKKKIRVLLLENDPEDADLVQKELNKTKFPFVFRRAEDEEGFRG
                     ALKEFLPDLILSEYCLPSFEGASPLDIAKEIAPEIPFIFISKQQGEDRAIESIKAGAT
                     DYVLKSGLSRLNFCVTRALREREAAEEQKQLRSELVKTLEQEGLILQGLPLALYSVSG
                     EAADRRISWVSEQVIRIFGYSAICFSDDPLFWTERLHPDDVERVWEEFRKIHESGHSV
                     VEYRWRCADGNYRWFLDEAVLMSSSGRPGEILGMWLDVTERKKLEQDFFRAQKMEAIG
                     RLAGGVAHDFNNMLTVILGYSDFLQEQLKEESLRADLAEIKKAGERASSLTRQLLAFS
                     RRQRLQPEVVNLNAVISDLARMLRRLIGEDIELVLTLDPGLRRVKLDPGQLEQAILNL
                     VVNARDAMPQGGRIEIATRNLLLKETFVCADGTSISPGRYARLDVSDTGCGMEPSVKE
                     KIFEPFFTTKDETRGTGLGLSTVYGLVKQSNGCVYVHSEPGRGSAFQLFFPTMETESP
                     VLEKKPERSGRVAGEGTILVVEDEESVRRLILSTLKQAGYETLSAGNGTDALEVAARH
                     EGRIHLVLSDVVMPHMSGRALTERLRQFCPDFKVLYMSGYNEETIQNCGIAGPDGNVL
                     QKPFTPDALVRKIREILEEDKVSPSVPG"
     gene            complement(21089..21637)
                     /locus_tag="P5079_00825"
     CDS             complement(21089..21637)
                     /locus_tag="P5079_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY28558.1"
                     /translation="MYNSIGGVETSSMAKGYDVTDTMLKTANVRLLLARTICPGKYVV
                     LVAGEVADVTMSVEAGASRAAESLVDRFVIPNIHPDVFPAVQGTAQVTARESMGLLET
                     FSVASLIEAADACVKAADVRLIEIKLAMAMGGKGLVTLTGSVAAVEAAIAAGAANAGE
                     KGLLTQTIVIPQPRPELFREII"
     gene            complement(21642..22985)
                     /locus_tag="P5079_00830"
     CDS             complement(21642..22985)
                     /locus_tag="P5079_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY28559.1"
                     /translation="MAKLSPADIVESIRNAGVVGAGGAGFPTHVKAASKAETVICNGA
                     ECEPLLHKDKEMLKAYPEKILAGLEMMMRSTGAARGVIALKGKYKELVEQYEKLLKGH
                     VAVRLQALGDFYPAGDEFSLVYDVTGKLIPPGGIPLQVGVVVDNVETLFNVAESPERP
                     VTDTFLTVTGAVKKPATLRVPVGLSIGEVVGLAGGAKAKEFAVLDGGAMMGRVTTDLS
                     EPVKKTSGGLIVLPKDHPLVRKKSAPRSAYSRIGQSVCDQCSFCTQFCPRYLLGYAVE
                     PHRVMRSLGFAGAERRPLSEWALLCCECSLCSLYACPEELDPRNVCVSAKGDLREMKV
                     DWKSARLNTGRSSPAHPMRDFRKVPVARLTRRLGLYDYQAEAHLKEDVGPVGKVRIPL
                     RQHVGAPASPVVSAGQKVSRGQLIAEIPANALGARVHASIAGTVTSVDAAITIEG"
     gene            23102..24040
                     /locus_tag="P5079_00835"
     CDS             23102..24040
                     /locus_tag="P5079_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRY28560.1"
                     /translation="MLKKLQIVGGKLVENGGDRPCVLVFVKPDDKERQQLLTDYHIDE
                     HTLASAMDPDEPSRIEIETDYLAVIFKHPKNYSERETLLFKVASMGLFLYKEQLVIVF
                     PDDIPLFTGKIFNSLENVNDIFLRVIYRSIYHFMEHLKVINMITDEIEHKISVSMENK
                     YLLNLFSLEKSLVYYLSAINSNEFVFEKLRNNGPKFGFSPKNMELLEDITIENSQCYR
                     QAEIYSNILASLMDARASIVSNNLNILMKTLNFITIAIMVPTFVVSAFSMNVRIPWEQ
                     HPQAFWIVMVLAVLSELGVMFWWQRATRFLHKKGRE"
     gene            complement(24110..26734)
                     /locus_tag="P5079_00840"
     CDS             complement(24110..26734)
                     /locus_tag="P5079_00840"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY28561.1"
                     /translation="MTSFIQKLFKDKKAAFMGLSLLGALAVAVFAQVSPEEAAEGGHE
                     GFQFFSFMTSPLYASYEKVALWVVLGVAFAGLLYALSLVKEVLGADKGTPKMQEITQA
                     IRQGADAYLKRQLTTVAILISVLVVLLYLSRALPTKNFGDPIAFGRAVAFFMGALFSG
                     LVGFVGMRLATTGNLRVAAAAKTSFGRALQLGYRTGTITGMLTDGLGLLGGTVIFMAF
                     GEKAYEVLLGFGFGGTLLALFMRVGGGIYTKAADVGADLVGKIEVGVPEDDPRNAATI
                     ADNVGDNVGDCAGMAADIFESYEVTIVAAMILGWASFGHKGVIFPILVRAIGVFASII
                     GTYLVRAGDKGSAGEAMKSINVGFWVSSVISIIGFVVLGFFYLRFDADYFARFPQVAA
                     VSQNLPWLFTFGVPGLDMRAAWTCFIGILLAVALNRCTEYFTGTEFAPVKGCVRACAT
                     GHATNIIEGLAVGFESSVWAILIIAGSIFASAVVYANTNPIFVAFGVAMCGIGMLTLT
                     GNTISMDVFGPVADNANGIGEMGYDAKEMGPENYKNARQILADLDAVGNTTKAITKGI
                     AIGSAVIAAVSLFASFITVIASGGQGEDAQVPIELYNAVAGLLKISDPTLLIGLLIGG
                     AVPFLFSSMTIRAVGRAAYLIVQECRHQFKVPGVMEGTKLPDYGKVVGICTASAQKEL
                     IGPGMLAVMTPILVGFALGPMALAGFLAGTILSGQLIAVFMANAGGAWDNAKKCIEDE
                     PRDPAKNTGKGSERHKAAVTGDTVGDPLKDTSGPAINPLIKVMNMVALLVVPLVIPFS
                     KPTIETLKSTAETLFQAQQIPQHFYDRLMAMIAAPMGGLSVTIIVVALSTIVWAVWQS
                     KRESEEMRKAHADIAGGK"
     gene            complement(26983..27882)
                     /locus_tag="P5079_00845"
     CDS             complement(26983..27882)
                     /locus_tag="P5079_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28562.1"
                     /translation="MKFLRLMMALALTFGVVGCGNNKSKNKVLGGVLYDGTSSSSSSG
                     VSTPNTPAGQAADAAQVAAQVAQVAGTTGSAMGAPAARRIGPGGGQPGGGGGQPGGGM
                     PSWGAMGSDGYYTLVFPNDPQFGSAPTVKIRFEKTDGTAVAFFTILESLMSGGMPDPT
                     TLATIQAITRVRQRVSGTDPGGNTFTATMTAPFGVPGGAGGSMVPSDMSMTGTIDVSG
                     PDGTFHFDITETVSPANNTMSGTLTGTGTLPDGTTVNINLTMTNGVVTGTVTVGGETV
                     QVDIDQNGAGNYRDSTGTHQLGD"
     gene            complement(27913..28803)
                     /locus_tag="P5079_00850"
     CDS             complement(27913..28803)
                     /locus_tag="P5079_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRY28563.1"
                     /translation="MKLKQLMIGIVTLSVGCVSVLSAQPTLVTETVSMGEAKSVSVEL
                     RPTYRVDLWKVTDTSTEYTDTVMRTDLNARWVPVEKWEVLLEVPHVSDKLKTEVGTAS
                     SDEKDSGIGQVILGGKWAFCPKGLFAGTLRVELPTADQDKLLGEGLNIGLGLAGEKAL
                     GPVMMYANAGYLVKQEYKTTFDTPGAAKTKEDPGDIVQLNAALSYPRWGLDWVGEVNA
                     NIVGREKDDATGSMKEVDGSAGTAVDLVLGVHKMFNESWSVKGGVAWGVGDEKIRTVD
                     MLRGAGDYKVILAGDYRFNY"
     gene            29036..30298
                     /gene="amrB"
                     /locus_tag="P5079_00855"
     CDS             29036..30298
                     /gene="amrB"
                     /locus_tag="P5079_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY28564.1"
                     /translation="MALLAGVPLPPLRHDLDIVPVQHEGKPMLLVSDLLGLEDKPALV
                     SPPVLALASFFDGKKRAREVQAELAKENATLSESEIQDVADQLDKLGLLETAAANETR
                     ARALEEFRNSPVRKQPFRGLPDNPLDMAALLNGFSSAPKGPGDWPNGNPSGKPPLGLL
                     CPHIDFARGGPLYAWSYRELARRPPPDVIVALGVAHLPPNSPWAFTRKAYETPFGPLP
                     VAEDLYRDITESLWYDPVVDEWAHAKEHSLEFQALWLKHLWREKTPKWVPLLCSSYER
                     YCVNAPPSSVPTVENAIQRIGDVLAARAKKGQSVLVLAGVDLSHVGRRFGDGMEITDE
                     LKKKIESFDRQSLEKALALDADGFFLQGTGENAWRKVCGLSAIYTSLRWIKSLAGGAR
                     VKSSLLAYDMAPDPAGGEVSFASAVFET"
     gene            30670..31749
                     /gene="rsgA"
                     /locus_tag="P5079_00860"
     CDS             30670..31749
                     /gene="rsgA"
                     /locus_tag="P5079_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRY28565.1"
                     /translation="MDLKKFGWTEESFPPPPQDKPYCAPARVVEEQKGGYYVVAATGE
                     LWAEVAGRLFYEASSRLHLPVVGDWVWIHPALPDTLSLIHGILPRKNGLSRLGMGRDA
                     ATGEEQPLAANVDTMLIVTSLNKEFNPSRLERYGTLAGQNGIRPVVILNKADICDDPR
                     PYVTAAREALPGAAVLTVSAATGLGMETLSELLTPGSTGVLLGSSGVGKSTLINRLLQ
                     EAVQDTMEIRADDHRGRHATTTRSLFRLPSGALLIDSPGLREVGLPQTGQGLPSTFSD
                     IEQLSANCRYRNCSHAREPGCAVQEARRLGNLSERRYLNYLKLLKERAFIKRKTDKGA
                     AREHKKYEKRISRLIKEYFKQGRPG"
     gene            complement(32021..32659)
                     /gene="rdgB"
                     /locus_tag="P5079_00865"
     CDS             complement(32021..32659)
                     /gene="rdgB"
                     /locus_tag="P5079_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRY28566.1"
                     /translation="MTKFSALVVIATQNRHKVREIRKILGRPSFAVRGLDDFGLAYKV
                     RETGKTLAANALLKARAAAGRTGRLCLADDTGLEVDALHGAPGVYSARFAGPACSYED
                     NNRKLLRVLDGEKRRTARFRCVVAVVLPDGRERLFEGVCAGRILRERRGSQGFGFDPL
                     FQPAGHKKSFAEMSLRQKNRISHRSRAFLKARKFLQGLYRRSPSLFAVSSEK"
     gene            complement(32678..33397)
                     /gene="rph"
                     /locus_tag="P5079_00870"
     CDS             complement(32678..33397)
                     /gene="rph"
                     /locus_tag="P5079_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRY28567.1"
                     /translation="MRPLKIVRPYLRHAEGSCLFQMGNTRVLCVATVETRLPPHAEEK
                     GIGWLSAEYGMLPRAGDKRTPRGRAAGGGRAQEISRLIGRSLRAAVDLQALSPFSVVV
                     DCDVLQADGGTRTASINGGFIVLVDALRKLYRDGRITTWPVKDYLAAVSVGLCKGKIE
                     LDMSYEADHDADVDLNMVMTGSGRFVEIQGSAEGRVFSRQELDRLLNAGRKGLKKVFN
                     LQKKVLRSIAPPPRAAGGISF"
     gene            complement(33411..36011)
                     /locus_tag="P5079_00875"
     CDS             complement(33411..36011)
                     /locus_tag="P5079_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28568.1"
                     /translation="MPAEINALLHNPLMLGVGLAGFGLLWFAVAAVISIVLPGGGGSE
                     DEVPVRTLPVSTVPSPDLRKIRFRMDDLYGYLQLRIAELEKEGPSAVVPPEVRQFNQR
                     VERLKADVEEIRRSLGTKVLWGAASSHLKDRVEDVARRAETMLSPDPAPAAVPAPSFG
                     PVAAEVEKFLADVRKEREGFLERERSADRASAVSKESLARESAQLRESLEKAGAKAEA
                     LKVEEEQLVRERDRREQMEKEIESQRGTDPLLEKLRQLEAENARRKAAAAAQEEAAHR
                     QRMRYRQRMDRLSARLVRWRTGWEARLGAHGNVLKSEEQKLQQETARLRAEWDLARRA
                     SEENFRRLEDERARAQLDKVHFEADVKGVWLQEKAEREKRLEGLEREKSLLEGNIEAV
                     QKQQVEEKERFAEALAAQRRAAREQQARWKEQLTAIQSAAARQSTLAAEEVQKVREAM
                     EYERQAVEKDLETQRALLNKETAEWTANKSQFENMAAEQRRALEERLAATSGHIKELQ
                     EEHGRLRAELDGQTREYALRREDQRRWAEQRFRTLNALLENTRKELSARLEKLREEKS
                     VLASSLEKERRRYQETLSDQTAASQVRQAELRAQRQSAEQSHAEALSRLKADEEKRRA
                     ELSEMERTLSSLEKKLTAFRQDSARRLRQQRDRYERLLLVMEQKASQEKVTGEARHAQ
                     EKLALESLEKRVANRRARLEKVLDRRRTELERLFVEAEGALEVVRQGHFQEARAWSAQ
                     LEELQRQRQSLEAKRAGLEKALGPEALEQQLKDLSDRFEAEKARLAREGEEYGRASQE
                     KSAAHHEAMQKAFHALEEAGEDLHRQETAAVDFLAAVQKHVSHLQSLRDYFRQRENRM
                     QRPSSSSSVA"
     gene            complement(36040..36804)
                     /locus_tag="P5079_00880"
     CDS             complement(36040..36804)
                     /locus_tag="P5079_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY28569.1"
                     /translation="MIFPDRRSFRRVLGAAVVGVLAGSAVAAASEDVSIRWLGQSCFL
                     ITTPDEVRVLLDPVPASVGYENPPQAVDVVTVSHEHKDHAGLSLPVGAYSVLHAVGPN
                     GNDWNRAQFEIGDARIYTVPSYHDKEKGAQRGLNGIFVVELPNFKLVHLGDLGHVLDD
                     GQIPPLRGADVLLVPVGGRYTIDAADAARVVEQIQPRSVVIPMHYRTPASKVKDLAGA
                     DDFLRGKNVVRVGGPEYKFATLTPPRQRTYVVLDFK"
     gene            complement(36791..37696)
                     /locus_tag="P5079_00885"
     CDS             complement(36791..37696)
                     /locus_tag="P5079_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY28570.1"
                     /translation="MKIRFWGTRGSIATPGQDTVKYGGNTSCVEMSDDDTLLIFDSGT
                     GIRPLGMDLQKRFRGTKIHGHLFITHFHLDHIQGFPFFMPVYVPGNSFTIYGCEGAGK
                     KLENIFVGQMSPEYFPVTLREMPAELKFIQLTTRPVTVNKWTVFPTYVNHPGLALGYR
                     VEDGRHKVAFITDNEPFRYLLRQQGNLLPVYDDIEKGSIQLEREDHNLCDFLRGVDVL
                     IHDAQYTTDEYKTKLSWGHSFYEFAIEMAVQAQAKQLVFFHHDPMRPDRQIDEFLVKY
                     QQNLQERKIPLIVHAAWEGMTLDLP"
     gene            complement(37775..>38465)
                     /locus_tag="P5079_00890"
     CDS             complement(37775..>38465)
                     /locus_tag="P5079_00890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY28571.1"
                     /translation="DYLTLMSQIVEKNKGVIDKYIGDSVMALFGAPVNHEGDVDNGLD
                     TAMEMLWKLKEFNYRRAWEGKPAVTIGIGLNTGPVVAGNMGSEKRLNYTVLGDAVNLA
                     SRLERLTRIYSVHCLVSEFTQRESRRGFVFRELDLVRVKGRSTAVRIYELCEQGENKD
                     RQTEMLQRFQNARQFYLLKDWERSARLFKQVLELCPGDGPSQLYLSRIEQLAKSPPPE
                     TWDGVYYYASR"
BASE COUNT         7260 a        12888 c        11304 g         7013 t
ORIGIN      
        1 gccatggccc gcgcggccgc gccgatgttc aaagggttcc tcggccgcac cagaacgacg
       61 cggagacgga tcgccttcgg cggcgtcatg ccgttcccgc tctcgacgag acggattccg
      121 tcgaccgcgg ggcgtcgccg atcttataga tgatcagaac acccgcggcc acgacacacg
      181 cggcaccgag aacccccaac ccatcgggca gctgccccca caaaaaccag tcccaaagac
      241 cggcgaaaac cacggtggcg tagatgaacg gaccgatttg cgaggcgggc gccaggccat
      301 aagcccgcgt gaggcacaat tggccgacgc tagcgcaggc ccccatcccc gccaggaaca
      361 accacagggg agcggacggg ctcacccagc ggcccgccag aggcgcagcc gaaataagcg
      421 tggaaaaaag actgaaataa aaaacgatgc ggaccgtggg ttcggtcaac gtcaaccggc
      481 gaatgccgac caacgccatg gccgccaaca cccccgaagc cagcgcgaga caggccacgg
      541 gctgaaaaag tcccaacccc ggtttcaaga taaggaggac ccctaaaaat cccagggaca
      601 ggaaccaagc caatcccgac ggaattttct ctcccagaga gaaatgggcg aaaaacggca
      661 tgaaaagcgg cgaggtgtag ctgagcagga gggcatccgc cagccgcatg cggccgatgg
      721 cgtagaacga acaatacatg gcggccagac cgaagacgcc ccgatacagg tgttgcagga
      781 tgtattttgt tttaaggctg ccgaacccgt tacgcagcac cagcggcatc agcgccagga
      841 ggccgaacgc gttgcgaaaa aaaacgattt cttcggaggg aagggcctcg gaagccactt
      901 tgatggccgc tcccagggcg gcgaacagaa aacacgccgc cacaagcagg acggaagctc
      961 ttttcaacat ggtcggattt ttcggtggac cggcgaaacg cggtcccgtc agaaagggat
     1021 caccacttta tcttttcgat gcgggaaagg gcctcccgca ggcgggggac cggcacggtg
     1081 agcgccagac gcacgtatcc ttcgccggaa ggaccgagcc cgttgcccgg cgtggccaca
     1141 atgtgggcct cgtccaagag gcgctcgacg gtcttggccg aggaataccc ctcgggcaca
     1201 tggacccaca cgtaaaacgt ggccgggggt tcaaaaacct tccatcccag ccggcgaagt
     1261 ccgccgacca ggacgtcgcg ccgttcttta taaatgcgcc tcatctccgc cacgcaatcc
     1321 tgcggcccgg aaagcgccgc caccgcggcc tcctgcaccg cctgaaaaac gccggaatcg
     1381 tagttgtctt tcaggcggcc gagggccttg accacctcgg cgttgccgca cacccaggcg
     1441 gcgcgccaac cggtcatgtt gtaggtcttg gaacaggaat ggaactccac cccgatctcc
     1501 ttggcgccgg ggatggagag gaatgaaacc ggcggttcgc cgaaatagat ctccgaatag
     1561 gcggcgtcgt gcgcgacgat aaaatcatac ccacgcgccc acgccaccac cttctcgaaa
     1621 aatccgcggc cggccaccgc ggaaagcggg ttgctgggat agttcagata caggatcttc
     1681 gccttgcgca gcacctcctt cgggatcgcc tccagctggg gcaggaagtc gttcttttcc
     1741 aggagcggga tgaaatgggg cacgcccccg gcgagcaaaa ccgcgctgtt gtaggcggga
     1801 taggccggtt ccggaatgag gaccgcgtcg ccggggttca cgaaggccag agggaaatgc
     1861 gtcagcccgt ccttggagcc gatgagggcg tgtatctccg tgtccggatc gaggacgacg
     1921 ttgaaacggt ttttatacca ttgggccacg gcgtggcgga acgtcttcag ccccgcaccg
     1981 aaaggatatt gatggttggc cggaacggcc agcgccttct gcccggcggc gacgatgtgg
     2041 ggcggggtgg gcaggtcggg gtcgccgacg ccgagagaaa tgatgtccgc tccccgcgcc
     2101 agggcggcct tcttcttctc gtcgatggcc acaaaaaggt acggcggcag gctctccagc
     2161 cgctgcgata tcttcgcttt catttttttc tcccgtcgga ccgcgtcgtc aaacccgcaa
     2221 aatcaaaagg ggggggtctt tccgtcggcc ccgtcgttct tctcaaaaat cttccagagg
     2281 accgccagcg caagcatcag cagggccagc gcggtcacgt tcacgaccca gccttcaaaa
     2341 atccccagtt tatacggata cccctcgtag ccgaaatcgc tccaggtttt cgaggtgagg
     2401 agcacctgga aaagggggac gatcttgacg aaaatccccc cttcgcagac caacagggac
     2461 cacaaaagcc agtacaggat gcccgaaacc gccggcacca ccaccagaac gatcgccacg
     2521 tggaccgggt cgtaaacgac gtcgtcgggg gagacgtctt cgccgaaaag ccacgccacg
     2581 taccgccgga cgcgcgccgg ccacgacggg gatgcgcgca ccctagacca cgcggttgag
     2641 gagagaaccg atcttttcca cgcggatctc cacccggtct ccccgtttca acgggcccac
     2701 gcccgacggc gtgcccgtgg agatgatgtc ccccggctcc agcgtcatca cctggctgat
     2761 gtgggacacc agccggctca cgccgaagat caactgggag gtgcgggagg actggcggcg
     2821 ttcgccgttc acatacgcct cgatcttcag gtcctcgggc gagatgcccg acacgatcca
     2881 cggccccagg ggcgcaaacg tgtcgaaccc tttggcccgg gtccactggc cgtcggaccg
     2941 ctgcaaatcc cgcgcggtca cgtcgttcat gagggtgtac cccaagatat atttcttcgt
     3001 cccctccaac ggcacgttcc gtgcgcggcg gccaatgacc accgccaatt ccgcctcgta
     3061 ttccacctgc cgggaagccg cggggaggcg gatgggttcg tccggcccga tgacggcgct
     3121 ggccggtttc aaaaagatca gcggttcctt gggaatctta tgtccgaact cacgggcgtg
     3181 gtcggcgtag ttcaaaccga tggcgatgat cttcgtcggc tcgcagggcg ccaggagttg
     3241 cacgtctttg aggggccaga ccaagccggt cggcgaagaa tcaccgaacg gatcgggcaa
     3301 cagttcctcg acggtgccgc gggaaacgcg gccccaccgg acggctcttc ctgtctgaaa
     3361 ccggcagaaa atcatggccc ggggcgccct actttccggc cttctgcttc cggtcggaaa
     3421 ggcccagcac gtcgcgcatg tcgtagagtc ccggctttcg gccgacgacc caatgggcgg
     3481 cggccaccgc gccggtggcg aaggcctcgc gcgtggtgat gcggtggatc aactccagcc
     3541 gctccccgtt gccgaagaaa aagacggtat ggtcgccgac cacgtcgccg ccgcgcagcg
     3601 cgtggacccc gatctccgcc gatcggcgcg cccccacctg tccctgccgt ccatagacga
     3661 acgcggaacc cggccggcgg ccgcgggcga tggcctcggc gagacgctgg gccgtgcctg
     3721 acggcgcgtc ctgcttcagt ttgtggtgga cctccgtgat ctccacgtca tagtccggaa
     3781 ggagtttcgc cagggactcc gccacatcga agagcagatt ggcgccgaag ctcatgttcg
     3841 gcgaaaaaac caccgggacc ggtttgatcg tcttcaggaa ctcgtccttc tgcgttccgc
     3901 gcaatccggt ggtgccgacg accatcgctt ttttggcggc cgccgcgaga gccgcgttct
     3961 tcagggtgga ttccggcgag gtgaaatcga tgaccgcgtc gcatttttcg atcaccttcc
     4021 ccaggtccga cgatacgacg acgccctcca gttccttccc caccagccga tgtcccttgg
     4081 actccaccag tcccgccagg gaaactccct tgtcgcccgc ggcccgcgcc aggatggccg
     4141 ctcccacccg tccaccgacc ccgcaaatcg ccagtctgat catattttca ttcctttctc
     4201 gctccacgat caaccgcgga ccgacggcgc ttccgacaaa ccgtaggcct tcatcgcctt
     4261 caccagtttc tgtttagcgg tttcctccat gggacaaagc ggcaggcgca gttcgccgga
     4321 acaaagcccc agccactcca ccgcggtctt caccgggatg ggattcgtct ccaaaaagag
     4381 cgccttcgtc aggggagtca tccgcgcatg aagctcctgg gctttttgcg tatcgccctt
     4441 cttcaacgcc tcgcacaacg cgacaacgtc cttggggatg atgttggcga tgaccgacac
     4501 cacacccttt ccgccgacac cgtacatggc gggagtcaac gcgtcgtccc cggaaagaac
     4561 gtcgaacttc ggaccgaggg cctgaacgat ctcacgggcc tgatcggtgc cgccggaggc
     4621 ctctttgatc cccacgatgt tcttctcgct gcgcgccaac tcgatgaccg tggcggggag
     4681 catgttgagc cccgtgcggc cggggatgtt gtagagcacc tgcggcaggg aaaccgcccg
     4741 ggccacggcg cgaaaatgtt cgatcaatcc ccggggagtg ggcttgttgt agtaggggac
     4801 caccagcaag gccccgtccg ccccggcctc ctgggcatgt ttggtgaggg cgatggcctc
     4861 ggtggtggag ttggatccgg tgccggccat caccaggcac ctcttgcgcg caaacttgac
     4921 gacaagatcg atgacgcggc cgtgctcctt gtgcgacatc gtggcggact cgcccgtcgt
     4981 gccgcaggga acgatggtgg aggtgccgcc gctgatctga aactccacaa gttcggcgag
     5041 tttcttctca tccaccacgc cgttcttgaa cggtgtcacc agggctacgc aggaaccttc
     5101 aaacataatt cctcctgaga tatgctccta aattcacgcc cgattgccgc ggatccgccg
     5161 caactttctt cgggggcgtc gctccgcttc agagaaaaaa acgaccgcgg aaaacgtatt
     5221 ccgccggacc ggtgagataa acgtggtcgt ccggcgccca ctccacccgc aagacgccgc
     5281 cgggcagagt caacgccgcg gcccggcgca gccggccggt caaaacgccg gccacggtca
     5341 cagcgcaggc gccggtgccg caggccatcg tcaggccggc gccgcgctcc cacacgcgca
     5401 cccgggccgt ttccggcccg gtcacttcaa cgaactccac gttcgtccgt cgaggaaaaa
     5461 acggatgcgt ctccaacgcc gggccccaga cgtccgccgg aagatcctcg acgcctttca
     5521 taaaaataac ggcgtggggg ttgcccatgg agacacaggt aaaaaacagc ggggaacgcg
     5581 gtaatctcaa tttaagattc ctcaaagagg ccaacggcct ctcgatgacc cgcccccggg
     5641 cgcgggtcgg aatacgggcc gcctccagga tgggcgcgcc catatccacc cgcacgccgc
     5701 cgtccttccg caagacgggc cggacgacgc cggccaacgt ctccacggtc atgtcgtcgc
     5761 gccgcacatg gccgttgtcc cgcgcgtagc gggccaggca gcggatgccg ttgccgcaca
     5821 tctccacctc ggacccgtcg gcgttgacga tcctcatgcg caggtcggcc gtggcggacg
     5881 gaagaaggat caggatctgg tcggccccca cgccgaaatg ccggtcgcac agccggcgtg
     5941 ccagagcggg cgtcatccgg acggagcggc gcatcccgtc aagaacgatg aaatcgttcc
     6001 cggtgccgtg catcttggtg aaggagactt cgcccgccat ccggctcccc gctctcataa
     6061 agtcatttca acgccggcgg gatcttttcc ccgcgcacca ggtcgttcaa cgtctcgcgt
     6121 tccctcacca cccaccactg cgaaccgaac acaaggacct cggcggcccg cgggcgggaa
     6181 ttgtactggg aactcatgct gaacccgtag gcgccggcgc ccaacaccgc cagaaaatcg
     6241 cccgcgtgcg ggcgcggcag aacgcgtccc ttggcgagat aatcgcccga ctcgcacacc
     6301 ggtcccacca cgtcggtggc cacgccccgg atcgacgaac gccgcaccgg ccaaacgggg
     6361 tgataggcgt cgtacagcgc gggccgcgcc aaatcgttca tcgccgcgtc caccacgacg
     6421 aaattcttat gggcggtctc cttgcggtag agcacgcgcg tcagaagaca gcccgactcc
     6481 gccaccaaaa accttcccgg ctcgaacacc agccgcaggt tccgcccctt catcatcggc
     6541 aggaggcggc gcgccagatc ggcgggagcc ggcggctttt ccccgtggta agggacgccc
     6601 aacccgccgc cgagatcgag aacatgcaga tagatcccat gagactgaag ccggtccacc
     6661 agagagagga gctttttcgc cgtctccagg tagggccgca cctccaggat ctgagaaccg
     6721 atgtgagcct ggatgccgac gatgttcagc cacggcttcc gggcggcgta tcgatagagc
     6781 tccacggcct tctcgtacgg cacgccgaac ttgttttccg ccttgcccgt ggcgatgtga
     6841 tggtgggtgt ccaccgaaac gttggggttg acgcgcaggg acaccggcgc cggacgacgg
     6901 agccgccggg ccgcggcctc gatggcctcc agctcttcgg cggattccac gttgaacatg
     6961 aggatccccg cccggatcgc cgcgacgatc tcggtctccg ttttccccac gccggaaaaa
     7021 acgatccggt gcggcgggat gcccgcgcgg cgggcgcgga cgagttcccc cacggaaacg
     7081 atgtccgccc cagccccttc ctcggccaaa agacgcagca ccgcaaggtt gctgttggcc
     7141 ttcatggcgt aacaaacagt gcgcgaacgg ccggcaaaaa cgcggtcgaa gttccggtac
     7201 tgtgtccgga tcttcgatgc cgaataaacg tacagggggg tgcccagacg ctcggccagg
     7261 gtccggaggg gaaccccctc cacccaaagt tctccgccgt cgtaatgata agtcccgtat
     7321 ccggtcttca tcgttattcc tttttgaacg ccgctcccct ttccgccttc ttttcaagag
     7381 ccgccaaaaa atcgtccatc aatccgcgca tgtagccgtc ggtcgttttc acggaagccg
     7441 tcttcatctc ctctttggcc ggagaatacc gacccgcctc aaactcggtc agcgccagca
     7501 gaaaccgggc cagagcatcg tcgggccaga ggcccaacgt ccggcgccaa tggcgcgccg
     7561 cctcgtcgtt cttcccctgc cgccaggcca ggatgccccg ttcgacgaat atccgcgggg
     7621 acttcaaccc gagcgattcg gccttatcga aggcccgctg ggcgagcgcg tactcgtcca
     7681 cgcgcgccca caaccggccc aaatagccgt attccgccgc cgccggctgg acggccaaaa
     7741 tcttctccca gcgccgggcc gccgcccccg tatcaccgag ggactcctcc acgacggcca
     7801 agagccgatg cgccccccaa tcctggggtt ccgccttcag atagcgttcc gccgtggacc
     7861 gagccgaatc gagccggccg gcgctgcaat acgaactcgc cagcgccagc agcacgtcct
     7921 tccggtgccg attttttttc agaatctttt catagatctc gacggcctgc gggtgaaggt
     7981 ccacgagcgt gtatatccgc gccagatcga gcatccggtc cgggtttccg gggcggagct
     8041 ggagcgcctg ccgataacaa tccagcgccc ggtcgtaaat cttgagggcc tcatagcaat
     8101 gccccagggc cggccaccaa cggacaaggt ccttttcggc ggcgcccggg accacagcct
     8161 tttccaggag cgccgcggcc ggctcgtatt ttttgccgga acgcagttct tccgcctcgg
     8221 cggcccaatg ccaccgggcc ttgcgggaag ggccttcgcc gccgcagacg cggccccagg
     8281 ccgctccgcc aaggagcagc gcgagcgcga cgttcttcca tcgccggacc ggcgttctca
     8341 cggcgtcttc ctcggctcgg cgggggtttc cagccaccgt tcaaagaact tcttgaccaa
     8401 cacccggcgc aaccgcggca acgattcacg ggcggccacc tcgcccgccg cgatgcattc
     8461 ctgcgaacgc caaagctcca tggcggaaac gtcttccact ttcggagcga tcagcacgtc
     8521 ggcctgtttc agacgctcct ggctgatcac ctctccctga atgtagaggg cctgggtgag
     8581 cgtcatgagg atgttggaaa cgttgtattt ggagaaattg gccggcacgg cgacgcacac
     8641 gatcacatcc gcgccgagca tcttcgccac gtccgtcggc acgttgtcca cgatcccgcc
     8701 gtccaccagc aggcggtgcc ggtaaggcac gggctggaac aaacccggca tggtggcgct
     8761 cgcccgggcc gccaacgcca cgctgccttc ccgcaaaacg atctgttccc cggtcttcag
     8821 gtccgtggcc acgcaggcga agggttttcc caaatcggcg aactgcttgt tgcccacgta
     8881 gcggccgatg taatgttcca tcttctctgt gcggagcagc tcctcggaca cgaacagacg
     8941 ccacacgcgg gcggcggtca tgtcggagat cttgtcccac ccgacctcct gtcccatccg
     9001 ttcgatctcc tcgacgggca ggcccgcggc gtacagagca cccaccaggg cccccacgct
     9061 ggtgccgacc accaagtcca cgggaacgcc ttcgcgggtg aggaccttca acacgccgat
     9121 atgggccaac ccgcgcgcgc cgccgccgcc caacaccagc gccacgcgcg gacggcgttc
     9181 caccggcaac cggcggaact gcgcccacaa cgcgtccgtc agctggtcct cttgtgccgc
     9241 cagcgaggtg tcgctcaaga cgtccagcgc cgaggcggac ggcgcggcga ggaccggtcc
     9301 cggcaggaac aggaggacgg ccgacagaac cgccgccgcg ccgcgccctc ttccgatcga
     9361 aattggattc aaaacgcgcg ccacggaccg gaaggaaggg ccgagccggt cattcgccga
     9421 gcgggcggcc gaccgccttc tgcaactttg ccgcggccac ccgttggccg tggaccgctt
     9481 cccaataaga ttcccgcaac tcgatgagcc aggtcttggc cctcagccgg tcgcccgcgt
     9541 ccttgccgtt ctccaggccc cgcgtcagcg actccgcctc ctgcagttcc ttctctcttc
     9601 ggaggcgttc gttctgccaa tagacgacgt cggcataact ttcccgcact tcgaggttca
     9661 cctcgtcctc gatctgggcg cggcggatgc ggctctggtt ggcctgcatc cgtttttgac
     9721 ggatgcgcgc gcggctggaa aacccgtcga agagcggcag gctcacgttc aaggcggcgc
     9781 tccattgggt cgtcttcagc gggaattcgg gatcgttcaa ctcataggcg gccccgaaag
     9841 ccaccaccgg gaaccgttcc gcctgggaca ggttgacggc caacttgtcc atttcctgct
     9901 gaaagtccgt ctggcggatc tcggaacggg actcctgagc ccacgccaac agtttggcca
     9961 ggtccatatc gaaagaaagc gcggtcaact ccccctggat gccgacctgg gtgtagagtt
    10021 ccacgccgag gacgctcaag aacgtcaacc gggccgattc caattcgcga cgccgttcgg
    10081 cgatctcccg gcgcagacgg ctctgcgcga cccccagttc cgtccggcga cgcacatcct
    10141 ccgacgacac actcaaggac aacgccgaca gggaattcag cgcctccacc cggatctgta
    10201 tctttctctg acacaacaac acgtcataga aggccttcgt cacatcgaaa accaaccggc
    10261 tgcggatctc ctcatattgg atccgcgccc gttccaaatt ggtgcgcgac aggcggatgg
    10321 tgttgcgcgt gcggccgccg ctgtagagcg gctgcttgag ccaggcccgc gccgaataga
    10381 acatgtccgg ctccagcccc cgcgagggcc gcagcaagac ggaaccgaac tccggcggca
    10441 tgacgtagtt ctgttcggcc agatagcggg cgctgttgaa gttcaatccc aactgcggaa
    10501 aatactgcga ttgcgcctcg cgcaggcgct gttcggagat gcggatatcc tcctgcgcca
    10561 tgagcaaacg ttggttgttc tgaagagccc gctcgatgcc ctgcgggagg gtcagcacct
    10621 gctccggcga cggctcctga cagacggccg gccgaggcag gcacagggaa caggccccca
    10681 gaaaagacag acttaggatg agctttcttg tcatttgttt aggacggaaa aatctatttt
    10741 ttgcgcagga ggcggcgcag ccgctggatc tgccgagaca cctcggccgg cgccgtgccg
    10801 ccgcgcgact tccgcgccgc caccgcaccg tccacggaca gcacctcgtg gacccatgtc
    10861 ccgaagagcg gcgaaaactt ccgcaacacc ggcaacggga tgtcctccaa aggcaaatcg
    10921 cgttcctggg cgtagttcac cgccgcggcc acgacgccgt gggcctgtcg gaacggcatc
    10981 ccccgccgca ccagcgcgtc ggcgaggtcg gtggccaaaa tgtatcccag ccggcaggcg
    11041 gcccgcatct tgtccgcgtt gatccggagg ctccgcacca tcggcgcggt cacgtccaac
    11101 gaggccttca ccgcgtccac cgcgtcgaac accggcggtt tgtcctcctg caggtcccgg
    11161 ttgtaggcca gcggctgtgc cttgagcagc gtgaggaccg ccgtgagcga tccgtagacc
    11221 cggccggtct tgccccgcac cagttccgcc atgtcggggt ttcgtttttg cggcatgatg
    11281 gaggatcccg tcacgaacgg gtccgccacg gtcacgaacg cgaaacaggg gttggaccag
    11341 atcatcagtt cctcacagaa ccgcgaaagg tgcgccatca ccagggacaa cgcggccaaa
    11401 aattccgcca ggaaatccct gtcgctcacc gcgtcgatgg aattctcgat cactcccgag
    11461 aaacccagcc gtcgcgccac ccattcccgg tcgatcggaa aggtggtccc ggccagcgcc
    11521 gcggcgccca gcggcaaaac gttcgtccgc cgccgcacgt cggccagccg ctcacggtcc
    11581 ctctggaaca tccaggcata cgccaaaaaa tggtgggcgg ccaggatggg ttgtcccggc
    11641 tgcaaatgcg tgtatcccgg catgacggcg cgccggttct tttccgccaa ctgaacgaaa
    11701 gccgcggaca aatcctccag ggaaccgctc aggacgtcga tctggtcccg cacatagagc
    11761 cggaacgccg tcacggtttg gtcgttgcgc gaccgcgcgg tgtgcatccg tcccgccgcc
    11821 gggcccgccg ccttgaagag ggccttctcg atggcgtaat ggatgtcctc cgcggccgga
    11881 aggcggacgc ctctcttcac ccggcccaag agcgccgcca ggccccggac aaggcggccc
    11941 ctgtcgcgcc cggaaatgat cctctgccgc cccagcatct ccacgtgggc gagggaggcc
    12001 gtcaggtcgt acaccgccaa acggtgatcg aacccgatgg aggccacgaa atcctccggg
    12061 cggacggtcg ctgttgtctg tcgattattc tttttcatgg ttcccatgga agaagtcccc
    12121 gccgccgggg cgcttccgat ttttcaccga actcctatcg attcttcctc agcagcgcct
    12181 tcaccttgag cggcaggccg aacaggttga tgaacccctc ggcgtcgcgt tggttataga
    12241 tctcttcccg ttcaaaagtg accagtttct gccaatagag cgagtagggg gagacgcgtc
    12301 ccttcaccac gatgtttccc ttgcacaggt ggagccgcac gcggccggtc atatatttct
    12361 gcgtctcggt gacgaacgcc tccagcgccc ggcgatgcag cgagggccag agcccgtaat
    12421 agaccagttc cccgtatttg gcgccgaccc cctctttggc atggaacgtg tcccggtcca
    12481 ggaccagcga ttccagttcg cggtgcgcgg cgtagaggag cgtggcggcc ggggcctcat
    12541 acaccccccg ggacttgatg ccaaccagcc ggttctccac catatccacc cgccccacgg
    12601 cgttccggcc gccgatcttg ttcaacaggt cgatcatttc cacgggtccg cggcggcggc
    12661 cgttcaccga caccggcacg cccttgacga attcgatttc gacggtttcc ccccgcgccg
    12721 gcgccttttc cggagacacg gtcaattcga acatgtcctc cttataagga tggttcgggt
    12781 cctcaagcac ccccccctca taggaaatgt gccacatgtt ggcgtcggag gaatagggtt
    12841 tggccttggt caccgtcacc gggatgttgt gccgttgggc gtaggcgacc tcgtcctccc
    12901 ggcctttgaa ctcccattcc ctccacgggg ccaggatccc caattccggg gccaacgcct
    12961 tgaaggtcaa ctcgaaccgg acctggtcgt tgcctttgcc ggtggcgccg tgggccacgc
    13021 cgtcggcctt gacccggcgg gccacctccg ccactttttc ggcgatcaac ggccgcgcca
    13081 gcgccgtgcc caacaagtag cgcccttcgt agagggctcg ggcctgcacg actttccaaa
    13141 tgtatttttc gacgaattcc cggcgggcgt cgacgacgaa acagcgggaa gcccccgtgg
    13201 tcagcgcccg tctcctcagc cggccgtaat cctcggcctg ccccaaatcc acgcagcacg
    13261 ccaccacatc gcagcgatag cgctcacgga tccatttcaa catgacggag gtatccaacc
    13321 ctcccgaata cgccacaacg acacgattga actttctcat gaccaggaac tcctccacag
    13381 agagaacggc ttgatttttc gacgcggttt acctttgtgc gggtgccaga ttttacccta
    13441 aaaacagggc ctcgtcaacg agcggtttta agccgttttc tataaaaaat ccccgggggg
    13501 tttcccgggg atcgccgttt ttcactcgat tttgtcgacg gtcaggtccg gaaaaaggtc
    13561 tcccgcggcg aaaacgccgt tttcgaccgg gttttacgaa aaaaaatccc cattccccgg
    13621 ctcattttgc ccgccttttc gacgagttct tcaaactcct cagcccttcc ttcaaaatcc
    13681 gcaggccgtg cgccgcctcg gcgtcggtca gacaaaaggg cggcaacaac cggatgaccc
    13741 gctcggccgt gcagttgacc agcagacccc tctcccggca aaaagcgacc acgtccccgc
    13801 cgggaatccg tagttccaat cccaggatgc accccgctcc ccggacttcc tttataaagg
    13861 acatctcctc ctgccatttc tcaaactcct cccggaacag ccggccgagc cgccgcaccc
    13921 gggccagggt tccggcgttc agggccttca tcaccgccaa ccccgctccg caggggaccg
    13981 ccccgccgcc gaaagtgctt ccgtgatccc cgggaccgaa caacgccgcc gtcttcttct
    14041 gggccagggt cgcgccgatg ggcaacccgt tggccaaccc tttcgccacg gtcaaaacgt
    14101 ccggaacgat accggggccg accacctcct tgttctgata ggcgaacagg gtgccggtcc
    14161 ggcccatgcc ggtctgtatt tcgtcgaaca tcaagagcag gtgatgcttt cggcagagtt
    14221 cccgaagccc ccgaaaaaac gccggcgggg cggtgcgaac gcccccctct ccctggatgg
    14281 gctcgatgag gacggcgcag gtccgggggg taattttccg tcggacggaa tccagatcgc
    14341 cgaactcggc cgccgggaac ccccccggca gggggccgaa atcctttttg ggatgtttct
    14401 gcgcggtggc cgccagcgtg gcgagggtcc gaccgtggaa ggagttcctg aagacgatga
    14461 tctgataccg gggccggccc cggtccggcg tgcggcagcc gtgccgccgg gccagtttga
    14521 tggcgcactc gttggcctcc gcgccggagt tggcgaaaaa caccttgccg ccgaaagtcc
    14581 gtcgggaaag ttcccgcgcc aggtcttcct gcggccgggt gtaatagagg ttcgaggtgt
    14641 gcaggaggat gttgacctgt ttggtcaccg cccgggccac gtcccggtcc gcgtgaccga
    14701 ggccgcacac cgcgatcccg gaaaaaaaat ccagataccg ccggcccttg tcgtcccaca
    14761 ggttcggccc atgcccccgg acgaacgtca ccggctgtct tttataggta ttgaactgat
    14821 ggctctcttt attcacggaa gaagcctcgt ccctttcatt ttcaaaagtc ccgcccggcc
    14881 gtcgatgatg tcgatctccc ggaccccttt ccggatggcc cgccggcagg aaagcacctt
    14941 gggaatcatg ccgccggtga ccacgccgcg gtcgatcaaa ccctgaatgt cccgcgtgtg
    15001 gattttcggg atcgtttttt tcctggcgtc cagaacgccg gcgacatccg tcagatacac
    15061 cagccggtcg gccttcagcc acacggcgac ggccgccgcc gcctcgtcgg cgttgacgtt
    15121 cagcgcccgg ccccgggcat ccgacgccac ggaggagatc accggcaaga attttccttg
    15181 cagcagggtc cgcaaaatcc ccggggccgc ccgcaacggt tcccccaccc ggcccaatcc
    15241 cttcacgggc cgcgccaaaa ggagcccccc gtcccttccg gacaaaccga ccgcccggac
    15301 gcccaaggaa cagagttccc ccaccagttt cttgttcacc tggccggaga ggacgccctc
    15361 caccaccgtc atggccgcgt cgtcggtcac gcggcggccc ttcacaaaaa ccggtttcac
    15421 gcccatcttc tgcagcgcgg cggaaatttc aggaccgccg ccgtgcacca acgccaccgg
    15481 acgccgtcgc gccaaccggg cgacctggcg caggaacgcc ttccgcgcct tgtcgtcgga
    15541 aagcaggctg ccgccgaatt tgaccaccca gacacttcgc gtcatgatcg tccctgtccc
    15601 tctcccgtcg ttgggagtca ttctagtaaa tccgcgcgct tttgcgcttt cgcgcgccgg
    15661 ggccggcggc tcaccctttg aactgaaaaa acgaaaggac gctgtccccg taatagtttc
    15721 gccggaacat ctcccacttt cccgaaacat cgtccaccgg ctcttttttg tggtgctggg
    15781 cgatgaccca cccgccggga gccaagagcc cggcttcctc cacgcgcatc agcgtggcga
    15841 tcgtcagcgc caagggactt ttttcggcgt ccttataagg cggccccagg aacacaaggt
    15901 cgaacggccg ccccttcagc caggcgagac ttttggtgat gtcgccgcag tgcacctcgg
    15961 cctggtcggc gaaccccaac cgggccgcgt tgccctcgat cgcccggcaa caacggcggt
    16021 tgccgtccac gaacacggcg cgggcggcgc cgttggacag cgcctccagg cccaccgcgc
    16081 ccgtaccggc gaaaaggtcc aggaaaaaag atcccgccag ccggggacgc aggatatcga
    16141 agagggattt acggatgcgc gccaggatgg gccggacatc gaccccggcg tgcaggccga
    16201 ccaggaggcg gccgcgggcg attccggact ggatacggag caccgcttac tctcccagga
    16261 cctgaacggt cacggcgcgg cggcggggcc ggttgtcgaa atccaccaga acgatctgtt
    16321 gccatgtgcc gcggaccagt tttccggaac gcaccggaaa aacccggctc tgtcccaaca
    16381 gcccggaccg cacatgggcg tagccgttgc cgtcgtgcca ggcgtcctcg tggtgatagc
    16441 gcccccgccg gggcgccagt ttctcgaacg cctcccgcag gtcgcgcaac acgccggact
    16501 cgtactccac cgcggtgatg cccacggtgg aaccggcggc gaacaccagc gcctgcccct
    16561 cccgcacgcc ggacgaacgc accgcttcct ccaccggttc ggtgatgtcc acgatgtccg
    16621 tctcgccttt cgtcgtcaaa ctcaggtcgg tcgtatgtgt ttttatctgt gtcatcttcg
    16681 cttcattttt ctccagtgtt gtgatggcgc ccggaaaaac cctccaaaaa ccgccgaagg
    16741 cgtcaaaacc ctccgccgcc gacggaggag agacacgttt caataaccga cactatcctt
    16801 tggaaaccgc cagctccatc cagagggaag ccatctccct tttttccacc ggggtcattc
    16861 cccgcttatc cacccaatac cttatttcag ggaacaagac caatcccggg accgccgtgg
    16921 ggatgacccc gcctgtgttt tccgacaaaa aaatccccag cccgcccaag aattcttgcg
    16981 atgtccggca tgcctgatgg gccaagaatt ttttgattgt aacaaattct attccaaacc
    17041 tctccggatg ccgccccatt tcagagtgag gctcaagccc gaaggtaaaa caattatagg
    17101 cccgcaccag cggcgccagc cgtttggcga cactccacgt atgtttggcc tcctcatagg
    17161 tcgtcgtcgg aaaatcccag atcaggtcga ccgaggtctt tattccgagt tctcccatct
    17221 cccctatatt ccgttccgcc gtttcggccg agtatccctt gttcatcgtt ttcaagacgc
    17281 gcccatcgaa cgactccacc cccaaagcga tctccgcgcc accgctcgcg gccacttttt
    17341 caagaagctc ggccgtccac ccccgagaac tccgggcgaa ggtccaccat tgcacgccca
    17401 tcgaatgttc ccgtaacgcc tccgccaaat ccttggcgta tcgaggcggc atttcgtccc
    17461 ccatcaattt gaatttccgc tgcccgtatc ggtcatgatg atgtttcagg tcgcgaacga
    17521 catcgccgac gggccggaaa taagcaccgg cccattgcct gggataagag caaaaaacgc
    17581 accgccccca agaacatccc cgggcgtaca acaacgggac atacctgtct tctgaataca
    17641 gcgggctcac tgaaaaatcg gaaaaatccg gaggagggtc cggctccgcc ggctcaacgg
    17701 gacggggatc gttccccacg atatttttcc cgctccgata gatcagcgcg ggcacgcatt
    17761 tcaagtcgcc gcgggacttc agaatccgga ccaattccag caacggcttc tcgcctgcga
    17821 accggacgat cccgtccacg ggaaccatcc tcattatttt ttcttgcgcc tccggcgtca
    17881 ttgtattcat cgtcgccccg cccaaaacga ggaaagcgtc cgtcttctgc gcccgcaaca
    17941 tccgaacaat ggcgatggcg gccccccact gggaggggag tgttatcgag aaaccgataa
    18001 tcttgtagtc cgtttcccgc tgaaggggcg gcatcactgt tcgtaaaaaa tcgtccatcc
    18061 caaacggcat gagcccatga cgcgccacgt attcccccag atcgtctccc aaatgctccg
    18121 gaaaagacag gggggcgtgc atcgcctgaa acaggaaatc caggagatcc tgcctgacgg
    18181 attccccccg ccaatcttcc cattggcttc gggatgtctc cgataactgc aggtaatgcc
    18241 gcaaggcgga cattttaagc gggtcatccg ccgtcttccg catcgcctct gtcgaccgac
    18301 gacgccactg tcccgccgcc ggcgacaaca accattcgac ataccgtccg ttcagatcga
    18361 gcgcgtcgac ggacacggac cggcgctcca aataggattt aagcatcggg accgcgagag
    18421 gaaactccca ggaatccctc acgaagggcg ggtggatgag gaggatgtct ttaggggcgc
    18481 tcatagggcc taagaagtca ttggatcgcg atgggtcggg gaaacaatcg agtggtaaac
    18541 cgtaaaaata aaaagtggag ctgaagggga tcgaaccctc ggcctcctca atgccattga
    18601 ggcgctctcc cagctgagct acagccccac aaaatcggaa tgtcctgtcg actgacaggc
    18661 cccattgtat cgaatctctt ccaaaaaatc cagagagcac cgggcggcct catccaaaac
    18721 agcgcgaggc cggcgggaaa aagaacgttc gtgaatgcgt ggaagacggg cggggaaacg
    18781 aaggggctcg gaacgcgttc ccctcaaccg gggacggaag gcgacacttt gtcctcttcg
    18841 aggatttcgc gtattttccg gacaagggcg tccggggtga acggcttttg caaaacattc
    18901 ccgtccggcc cggcaattcc gcaattctgg atggtctcct cgttgtatcc ggacatgtag
    18961 agcaccttga aatccgggca gaactgccgg aggcgttccg tcaaagcccg cccgctcatg
    19021 tgcggcatca ccacgtcgct gaggaccaga tggatgcgcc cctcgtgccg cgccgcgacc
    19081 tccaaagcgt ccgttccatt gcccgccgac aaggtctcat acccggcctg tttcagcgtc
    19141 gacaaaatga gccggcggac cgactcctcg tcctccacca ccaaaatcgt tccctccccc
    19201 gcgacccggc cggaccgctc cggttttttt tcgagaaccg gagactcggt ttccatcgtc
    19261 gggaaaaaaa gctgaaacgc gctcccccgg cccggttcac tgtgaacgta gacgcacccg
    19321 ttgctctgtt tgacgagccc atagaccgtg gagagcccca agcccgttcc ccgggtctcg
    19381 tccttggtcg taaagaacgg ttcgaagatt ttttctttaa cggacggctc catgccgcag
    19441 cccgtgtccg aaacgtcgag gcgcgcatac cggcccggag aaatggaggt gccgtccgca
    19501 cagacgaacg tctcctttaa aagaaggttt cgggtggcga tctcgatccg ccccccctgg
    19561 ggcatggcat cgcgggcatt gaccacgagg ttgaggatgg cctgctccaa ctggccgggg
    19621 tccagcttga cccgacgcag gccggggtcc agcgtcaaaa ccaattcgat atcctctccg
    19681 ataaggcgtc ggagcatccg cgccaaatcg ctgatgacgg cgttgaggtt gacgacctcc
    19741 ggctgaagac gctgccggcg gctgaacgcc agcagttgcc gggtgagcga gctggcgcgt
    19801 tcccccgcct tcttgatttc ggcgaggtcg gcccgcagcg actcctcttt gagctgctct
    19861 tgcaggaaat cgctgtatcc caggatgacc gtcaacatat tgttgaaatc gtgggccacg
    19921 ccgcccgcca gacggccgat cgcctccatt ttttgcgcgc ggaaaaaatc ctgctccagc
    19981 ttttttcgct ccgtcacatc gagccacatc cccaggatct cgcccgggcg gcccgacgac
    20041 gacatcagca ccgcctcgtc caggaaccaa cggtaattcc cgtcggcgca gcgccatcgg
    20101 tattccacga ccgagtgacc gctttcatgg atcttccgga actcctccca cacgcgttcc
    20161 acgtcgtccg gatgcagccg ttccgtccaa aagagggggt cgtccgaaaa acagatcgcg
    20221 gaatagccga agatccggat cacctgttcg ctcacccagg agatccgccg gtccgccgcc
    20281 tctcccgaaa cggaataaag cgccaggggc aacccctgaa ggatcagccc ctcctgttcc
    20341 agggttttga ccagctccga acgcagctgt ttctgttcct cggcggcctc ccgttcccgc
    20401 aaggcccgcg tcacgcaaaa attcaaccgg gaaagaccgc tcttcagcac gtagtccgtc
    20461 gcgccggcct tgatggactc gatcgcccgg tcctccccct gttgtttgga tatgaaaata
    20521 aacgggatct cgggagcgat ctctttcgcg atatccagcg gggaggcacc ttcgaacgac
    20581 ggcaggcaat attcggacaa gatcaaatcg ggaagaaact ctttcaacgc cccgcggaac
    20641 ccctcctcgt cttccgcccg ccgaaaaaca aaggggaatt tcgttttgtt cagttccttc
    20701 tgaacaaggt ccgcgtcctc ggggtcgttt tcaaggagga gaacccggat tttctttttc
    20761 atttcccctc tcccgcgtcg cgtgatcttt tctggacccg gcgcccggcc aagaggcttc
    20821 atttcttcat ctaaatcatc ggttccccgt ttcccggaga tgcttttata tatccccgaa
    20881 aggcccgcct gtcaacgact tttgttcgtc gtgaccgttt tgacgagatt ttattaaagt
    20941 ggattcccgg cggaaacgcg aaatatccgt tctctgtgga aaagaagcgc ggggattgat
    21001 tttttaggac ctactttgga gccggcgggg atgtaaattt atcgccaaca acgcgccccc
    21061 ggcgggcaaa aaccggggac ttcgaaactc agatgatctc gcggaaaagt tccggccgcg
    21121 gctgggggat gacgatggtc tgcgtgagca accctttttc gccggcgttg gcggcgcccg
    21181 ccgcgatggc cgcctccacc gccgccacgg acccggtgag ggtcaccagg cccttccctc
    21241 ccatggccat ggccagtttg atctcgatga gccgcacgtc cgccgccttg acgcaggcgt
    21301 cagcggcctc gatgagcgac gccaccgaaa aggtctccag aagacccatg gactcccgcg
    21361 cggtcacctg ggcggtgccc tgaacggccg ggaagacgtc cggatggatg ttggggatga
    21421 cgaaccggtc caccagggac tcggcggcgc gggaggcccc ggcctccacg ctcatggtga
    21481 cgtccgccac ttctcccgcc accagcacca cgtatttgcc cgggcagatg gtgcgggcca
    21541 acaggagccg cacgttggcg gttttcagca tcgtgtcggt cacgtcgtag cccttcgcca
    21601 tggaactcgt ttcaacgccg ccgatggaat tgtacatgtc gttatccttc gatcgtgatg
    21661 gccgcatcca cggacgtcac cgtgccggcg atgctggcgt gtacccgcgc ccccagcgcg
    21721 ttggccggga tctccgcgat caactgcccc cgggaaacct tctgtcccgc cgaaaccacc
    21781 ggcgaggcgg gcgcgcccac gtgctgacgc agcggaatcc tcactttgcc caccgggccc
    21841 acgtcctctt ttaaatgcgc ctccgcctga taatcgtaga gccccaaccg gcgggtgagc
    21901 cgggccaccg gcacttttcg aaaatcgcgc atcgggtgcg cgggcgagga ccggcccgtg
    21961 ttgagccggg cgctcttcca gtccaccttc atctcgcgca gatccccctt ggcggaaacg
    22021 cagacgttcc tcggatccaa ctcctccgga caggcgtaga ggctgcacaa cgaacattcg
    22081 cagcaaagga gcgcccactc gctcaggggc cggcgttccg cgccggcgaa tcccagactc
    22141 cgcatcacgc ggtgcggctc gacggcgtat cccaacaggt agcgcggaca gaactgggtg
    22201 cagaacgaac actgatcgca gacggactga ccgatgcggg aataggccga gcgcggcgcg
    22261 gactttttcc ggaccagcgg gtggtctttc ggcaggacga tgagcccccc ggaggtcttc
    22321 ttcaccggct cggacaggtc ggtcgtcacg cggcccatca tggcgccgcc gtcgagcacg
    22381 gcgaactcct tcgccttggc gccgccggcc aaaccgacca cctcgccgat gctcagcccc
    22441 accggcaccc gcagggtggc gggcttcttc accgcgcccg tcaccgtcaa aaaagtgtcc
    22501 gtcaccggcc tctccggcga ctccgccacg ttgaaaagcg tctccacgtt gtccaccacc
    22561 acgccgacct ggagggggat cccgccgggc gggatcagtt tcccggtgac gtcgtagacc
    22621 aggctgaact cgtcgcccgc cggataaaaa tcccccagcg cctgcaggcg gacggcgacg
    22681 tggcctttta aaagtttctc gtattgttcg acgagttcct tatatttccc cttgagggcg
    22741 atcaccccgc gggccgcgcc cgtggaacgc atcatcatct ccagccccgc caggattttt
    22801 tccgggtagg ccttgagcat ctccttgtcc ttgtgcagga ggggttcgca ctcggcgccg
    22861 ttgcagatga ccgtttcggc cttggaggcg gccttcacgt gcgtggggaa cccggcgccg
    22921 ccggcgccca ccacgcccgc gttccggatg gattcgacga tgtcggcggg agaaagtttt
    22981 gccatagagg gtcctcattt tacaaaaatt cggcggcgac cggccgaaaa atcgccgctg
    23041 ttttgctttt ctctcagaat tgtgtttata atactcccca gaccggagga cggagaaggt
    23101 catgctgaaa aaactgcaga ttgtcggcgg caaactggtg gagaacgggg gcgaccggcc
    23161 gtgcgtcctc gtcttcgtca aacccgacga caaggaacgg cagcagctcc tcaccgatta
    23221 ccacatcgac gagcacaccc tggcctccgc catggacccc gacgaacctt cgcgcatcga
    23281 aatcgagacc gactacctgg cggtcatctt caaacacccc aagaactatt cggagcggga
    23341 gacgctcctc ttcaaagtgg cctccatggg ccttttcctc tacaaggaac aactcgtcat
    23401 cgtcttcccc gatgacatcc cgctgttcac cggaaagata ttcaactccc tggagaacgt
    23461 caacgacatc ttcctccggg tcatctaccg gtccatctac catttcatgg agcacttgaa
    23521 ggtcatcaac atgatcaccg acgagatcga gcacaagatc agcgtctcca tggagaacaa
    23581 atacctgctg aacctcttca gcctcgaaaa atcgctggtc tactacctca gcgccatcaa
    23641 ttccaacgag ttcgtcttcg aaaaactgcg caacaacggc cccaagttcg gtttttcccc
    23701 caagaacatg gaactgctcg aagacatcac catcgagaat tcccagtgct accggcaagc
    23761 cgaaatctac tccaacatcc tcgccagcct catggacgcg cgcgcctcca tcgtcagcaa
    23821 caacctgaac atcctcatga agacgctcaa cttcatcacc atcgccatca tggtgcccac
    23881 cttcgtggtg agcgccttct ccatgaacgt ccgcatcccc tgggagcaac acccgcaggc
    23941 gttctggatc gtgatggtcc tggcggtcct ttcggagctg ggcgtgatgt tctggtggca
    24001 gcgcgccacc cggttcctcc acaaaaaagg gcgcgaataa gaattccccg aaaagaaaga
    24061 ccccctcggt tcccaacggg agccgagggg gtcttgtttt aggaacctgt tacttgccgc
    24121 cggcgatgtc tgcgtgcgcc ttccgcatct cctcggactc gcgtttcgac tgccacacgg
    24181 cccagacgat ggtggacagc gcgacgacga tgatggtgac ggacagaccg cccatgggcg
    24241 ccgctatcat ggccatcaac cgatcgtaga aatgttgcgg gatctgctgg gcctggaaca
    24301 gcgtttccgc ggtgctcttc agcgtctcga tggtgggctt gctgaagggg atcaccagcg
    24361 gcaccaccag cagggccacc atgttcatca ctttgatgag cgggttgatg gccgggccgg
    24421 aggtgtcctt gagcgggtcg ccgacggtgt cgccggtgac cgccgccttg tggcgttcgg
    24481 aacccttgcc ggtgttcttc gccgggtccc tgggttcgtc ctcaatgcat ttcttggcgt
    24541 tgtcccaggc gccgccggcg ttggccatga acacggcgat gagctggccc gagaggatgg
    24601 tgcccgccaa aaagcccgcc agcgccatgg ggccgagcgc gaaaccgacg aggatcggcg
    24661 tcatcacggc cagcattccg ggcccgatca gctccttctg ggcggaagcg gtgcagatgc
    24721 ccaccacctt gccgtaatcg ggaagtttcg tcccttccat cacgccgggg accttgaact
    24781 ggtgccggca ttcctgaacg atcaggtacg ccgcgcggcc cacggcgcgg atggtcatgg
    24841 aggagaacag gaacggcacc gcgccgccga tgagcagtcc gatcaggagc gtcgggtcgg
    24901 agatcttcag caggcccgcc accgcgttgt agagttcgat gggcacctgc gcgtcctctc
    24961 cctgtccgcc ggaggcgatc accgtgatga aggaggcgaa cagcgacacc gccgcgatca
    25021 cggccgagcc gatggcgatc cccttggtga tggccttggt ggtgttgccc accgcgtcga
    25081 ggtccgccag gatctgccgc gcgtttttgt agttctccgg gcccatttcc ttggcgtcat
    25141 aacccatttc gccgatgccg ttggcgttgt ccgccacggg accgaacacg tccatggaga
    25201 tggtgttgcc cgtcagcgtc agcatgccga tgccgcacat ggccacaccg aacgccacga
    25261 agatggggtt ggtgttggcg tagaccaccg cggaggcgaa gatggaaccc gcgatgatga
    25321 ggatggccca cacgctggac tcaaacccca ccgccagtcc ctcgatgatg ttggtggcat
    25381 ggcccgtcgc gcaggcgcgc acacagccct tcaccggggc gaactccgtg cccgtgaaat
    25441 attcggtgca ccggttcagc gccacggcca ggaggatgcc gatgaagcag gtccaggcgg
    25501 cgcgcatatc caatccgggg acgccgaagg tgaacagcca cggcaggttc tgggacacgg
    25561 ccgccacctg cgggaagcgc gcgaaatagt ccgcgtcgaa ccgcaggtag aaaaagccga
    25621 ggaccacgaa cccgatgatg gagatcaccg acgacaccca gaaaccgacg ttgatggatt
    25681 tcatggcttc cccggcggac cctttgtctc cggcgcgaac caggtaggtg ccgatgatgg
    25741 aagcgaacac gccgatggcg cgcaccaata tcgggaagat cacgcccttg tgtccgaagg
    25801 aggcccagcc caggatcatg gcggccacga tggtgacctc gtaggactcg aagatgtccg
    25861 cagccatgcc ggcgcagtcg cccacgttgt cgcccacgtt gtcggcgatg gtggcggcgt
    25921 tgcgcgggtc gtcctcgggc acgcccacct cgatcttgcc gacgaggtcg gcgcccacgt
    25981 cggcggcttt ggtgtagatg ccgccgccca cccgcatgaa caacgcgagc agcgtgccgc
    26041 cgaacccgaa gcccagcaac acctcatagg ccttctcgcc gaaagccatg aaaatgacgg
    26101 tgccgcccaa aaggcccagg ccgtcggtca gcatgccggt aatggtgccg gtgcggtagc
    26161 cgagctgcag cgcccggccg aaactggtct tggcggccgc cgccacgcgc aggttgcccg
    26221 tggtggccag gcgcatcccc acgaaaccca ccaggcccga gaacaacgcg cccatgaaga
    26281 acgcgaccgc tcggccgaag gcgatggggt cgccgaagtt ctttgtcggg agcgcccggc
    26341 tcaggtacag gagcaccacc aacacgctga tgaggatggc caccgtggtg agctggcgtt
    26401 tcaggtaggc gtccgccccc tggcggatgg cctgggtgat ctcctgcatc ttgggcgtgc
    26461 ccttgtccgc gcccagcact tccttgacca gcgaaagcgc atacaggagg ccggcgaacg
    26521 caacgccgag caccacccag agggccactt tttcatagga ggcataaagc ggggacgtca
    26581 taaaggaaaa gaactggaac ccctcgtgcc cgccttccgc ggcctcttcc ggagagacct
    26641 gggcgaaaac agcgacggcc aaggcgccca acaggctgag acccatgaac gccgcttttt
    26701 tgtctttgaa caacttctgg atgaatgatg tcatatgcac ctcgtcagtt tagtcgtcgt
    26761 gcgctcggaa aaccgcgtga tcacgcttcg tcgccggatt tttcgatcgt ccacgagatt
    26821 tcggaaaaaa tgggatatcg gacaggcgtg ccgcgcacgc gccgaatcaa aagcaggagg
    26881 ataaatatac aaaatttttg acgaaaaata cgcccgcaaa aaagaaagga ggggccggca
    26941 aaggcccctc ctttcttaaa ctaagacgcg aaaccgacga ggtcagtcgc cgagctgatg
    27001 cgtccccgtc gagtccctgt agttgccggc gccgttctgg tcgatatcca cctgaaccgt
    27061 ctcgccgccc accgtcacgg tacccgtcac cacgccgttg gtcatggtga ggttgatgtt
    27121 caccgtggtg ccgtccggca aggtgccggt gccggtcaga gtgccgctca tcgtgttgtt
    27181 cgccgggctc accgtctccg tgatgtcgaa gtggaacgtg ccgtccggac cgctcacgtc
    27241 gatggtgccc gtcatggaca tgtcgctggg gaccatcgaa ccgcccgccc cgccgggaac
    27301 cccgaagggc gccgtcatgg tggcggtgaa ggtgttgccg cccgggtccg tgccgctgac
    27361 ccgctggcgc acccgcgtga tggcctggat cgtcgcaaga gtcgtcggat cgggcatgcc
    27421 gccgctcatc aggctttcca ggatggtaaa aaacgccacc gcggtgccgt ccgtcttctc
    27481 gaagcggatc ttcaccgtcg gcgccgagcc gaactgcggg tcgttcggga acacgagggt
    27541 gtaatacccg tccgatccca tggcccccca ggaaggcatt ccgccgcccg gctgaccgcc
    27601 gccaccgccc ggctggccgc ctcccggtcc gatgcgccgc gccgcgggcg cgcccatggc
    27661 gctgccggtg gtgccggcca cctgcgccac ctgcgcggcc acctgcgccg cgtccgccgc
    27721 ctggccggcc ggcgtgttcg gcgtggaaac gccggaactg ctgctggagc tggtgccgtc
    27781 gtacagaacc ccgcccaaga ccttgttctt ggacttgttg ttgccgcaac ccacaacgcc
    27841 aaacgtcagc gccagggcca tcatcaatct caagaatttc atgtatttat cctccgaaag
    27901 tcgttcgacg aatcaatagt tgaagcgata gtcgccggcc aggatgacct tgtagtcccc
    27961 ggctccgcga agcatgtcca cggtgcggat cttctcatcc cccacacccc aggccacgcc
    28021 gcccttcacg ctccacgact cattgaacat cttgtgcacg cccaggacca ggtccacggc
    28081 cgtgcccgcg ctgccgtcca cttccttcat ggatcccgtg gcgtcgtcct tttcccggcc
    28141 gacgatgttc gcgttcacct cacccaccca gtccagtccc caccggggat aggagagggc
    28201 cgcgttcagc tgcacgatgt cgcccgggtc ttccttggtt ttggccgctc ccggcgtgtc
    28261 gaacgtggtt ttgtattcct gcttcaccaa ataccccgcg ttcgcataca tcatcaccgg
    28321 gcccaacgct ttttcgcccg ccaaccccag gccgatgttc agcccttcgc ccagaagctt
    28381 gtcctggtcc gcggtcggca gttccacgcg cagcgtgccg gcgaaaagcc ccttggggca
    28441 gaacgcccat ttgccgccca aaatcacctg gccgatgccg gaatccttct cgtccgacga
    28501 cgcggtgccg acctccgtct tcagcttgtc ggaaacgtgg ggcacctcca acagcacttc
    28561 ccacttttcc accggcaccc agcgcgcgtt caggtccgtc cgcatcaccg tgtccgtgta
    28621 ttccgtgctg gtgtccgtca ctttccacag gtccacgcgg tatgtcggcc tcaactccac
    28681 cgacacgctc ttcgcttctc ccatggaaac ggtttccgtc accaatgtcg gctgggccga
    28741 cagcacggaa acgcacccca ctgaaagggt cacaatcccg atcatcagtt gtttcaattt
    28801 caaggatgcc tcctctgatt ttttgatttc ccgcacgcgg cggtgatgtc ttggcggaac
    28861 gtcgatcccg ccgtccgtcg cagcccctcg tccgtctcat gcctccacgt caaaggcgac
    28921 cacaccggtg aaggattcta atcaatgcgg gttaaagttt caagacgatt tcttgtgact
    28981 ttacgaccga cccgctcgct tccccaaaag gcgtcgggtt cgatataata aacgcatggc
    29041 cctgcttgcc ggcgtccccc ttccgcccct gcgacacgac ttggacatcg tccccgtaca
    29101 acacgagggc aaacccatgc tgctggtgag cgacctcctg ggactggaag acaaacccgc
    29161 cctggtctcg ccgccggtgc tggccctggc ctctttcttc gatggaaaaa agcgggcccg
    29221 ggaggtgcag gcggaactgg ccaaggaaaa cgccaccctc tccgaatccg agatccagga
    29281 cgtcgcagac cagttggaca agctggggct tctggagacc gccgccgcca acgaaacccg
    29341 cgcccgggcc ctggaagagt tccggaacag cccggtcagg aaacaaccgt tccgcgggct
    29401 gcccgacaat ccgctggaca tggccgccct cttgaacggg ttttcctcgg cgcccaaagg
    29461 accgggcgat tggccgaacg gaaacccttc cgggaaaccg cccctggggc tcctctgtcc
    29521 gcacatcgat ttcgcccgcg gcggcccgct ctacgcctgg tcctaccggg aactggcccg
    29581 ccggccgccg ccggacgtca tcgtggcgct cggtgtggcg caccttccgc ccaactcccc
    29641 ctgggccttc acgcgcaaag cctacgaaac ccctttcggc cccctgcccg tggccgagga
    29701 cctttaccgg gacatcaccg agtccctctg gtacgacccg gtcgtcgacg agtgggccca
    29761 cgccaaagaa cattccctgg aattccaggc gctgtggctc aaacatctct ggcgagagaa
    29821 aaccccgaaa tgggttcccc tcctttgttc ttcctacgaa cgctactgcg tgaacgcgcc
    29881 cccctcttcc gtccccacgg tggaaaacgc catccaacgg atcggagacg tcctggcggc
    29941 gcgggcaaaa aagggacaga gcgtcctcgt cctggcgggg gtggatttgt cgcacgtggg
    30001 ccggcggttc ggcgacggga tggagataac ggacgaactg aagaaaaaaa tcgaatcttt
    30061 cgaccggcag tctttggaaa aagccctggc cctggacgcc gacggtttct tcctgcaagg
    30121 cacgggcgaa aacgcctggc ggaaagtctg cggcctgtcg gccatctaca cctcgctccg
    30181 ctggatcaag tccctggcgg gcggcgcccg cgtgaaaagt tccctcctgg cctacgacat
    30241 ggcccccgac cccgccggcg gcgaagtctc cttcgccagc gcggtgttcg aaacgtagac
    30301 caaaattcag ccgcctcatt ttagaagatg cgttgccgac acggctccca tcaaaaacga
    30361 acgtttccgc cacctccccc ccacctcttc ccacgaaaaa attatcacgt ccctcttgcg
    30421 cgggatcggt ttgagggata gactttgggg agaagccatc ctttccggag aggttttatg
    30481 tcacgccgtc ttccctacgc tgtgtccccc ctccgggtgg gactgatcct cctcggctga
    30541 aaatcccatc catcgagggt cagcaccacc ctcacttccc ttatatggtc aataaggaag
    30601 ggcgtccttc ctttgtcctc gcggaaaatc aacctcctcc gcgctcttac atccgataag
    30661 gagaccatca tggacctgaa aaaattcggc tggacggaag agtcttttcc cccgccgccc
    30721 caagacaaac cttattgcgc accggcacgg gtcgtggaag aacaaaaagg cggttattat
    30781 gttgtggcgg cgaccggcga actgtgggcc gaagtcgcgg ggcgattgtt ttacgaagca
    30841 tcctcccgcc tgcatttgcc cgtcgtgggg gactgggtgt ggatccatcc ggccctcccc
    30901 gataccctgt ccctcatcca cggcattttg ccgaggaaaa acggcttgtc acggctgggg
    30961 atggggcggg acgccgccac gggagaagaa caacccctgg cggccaacgt ggacacgatg
    31021 ctcatcgtaa cgtccctcaa caaagaattc aatccgtcgc gtttggaacg ttacgggacc
    31081 ctggcggggc agaacgggat taggccggtg gtgatcctca acaaagcgga catctgtgac
    31141 gatccccgtc cctacgtgac ggccgcccgg gaagcgttgc ccggcgccgc tgttttaacg
    31201 gtcagcgcgg ccaccgggct ggggatggaa acattgtccg agctgctcac ccccggttca
    31261 acgggagtgc tgttggggtc ctccggggtc gggaaatcga cgctcatcaa ccggttgctg
    31321 caggaggcgg ttcaggacac catggaaatc cgcgccgacg atcaccgggg caggcacgcg
    31381 acgacaaccc ggtccctctt ccggctcccc tcgggggcgc tgctcatcga cagcccgggg
    31441 ctccgcgagg tcggcttgcc ccagacgggc cagggactcc cttcaacgtt ctcggacatc
    31501 gaacagctgt ccgcgaattg ccggtaccgg aactgttctc atgcccgcga acccggctgc
    31561 gccgtccaag aggcaaggcg actcggcaat ttgtctgaaa ggcgttatct caactacctg
    31621 aaattattga aggaacgggc tttcatcaaa cggaagacgg acaagggggc ggcgcgcgaa
    31681 cataaaaaat acgaaaaacg aatcagccgg ctcatcaaag aatatttcaa acaggggcgg
    31741 cccgggtagc cggtcgccaa aaaagttctt ttgggctgtc attgcgagga gtcagcgacg
    31801 aaacaatccc acctctccgt tgaaaacggc aagatcgccg cgctccatcg gagtattcgt
    31861 tcctctggca cgccgttggc cccaaaagct ctgcgtgggg acctttggtc gctcgcaatg
    31921 acgacacggg agttttcagc atcccgctga ttgcggcaac aattcgactc cgtccagacc
    31981 ccggcgcgca ggggaatcgg acgggattga ttcaggaagg ttatttctcg gaagaaacgg
    32041 cgaagaggga cggggaacgg cggtagagac cctgcaagaa ttttctggcc ttgagaaaag
    32101 cgcgcgagcg atggctgatg cggttcttct gacggagcga catctcggcg aaggattttt
    32161 tgtggccggc gggctggaag agcgggtcga aaccgaatcc ctgagaccct cgacgttcgc
    32221 ggaggatgcg gccggcgcag acgccctcga acagcctttc ccggccgtcc ggcaaaacca
    32281 ccgccaccac acagcgaaac cgggcggtcc ggcgtttctc gccgtccagc acccgcagaa
    32341 gtttccggtt gttgtcctcg taagaacagg ccgggccggc gaagcgggcc gaatagaccc
    32401 ccggcgcgcc gtggagggcg tccacttcca ggccggtgtc gtcggcgaga cagagccggc
    32461 ccgtgcggcc cgccgcggcg cgggctttca ggagcgcgtt ggccgccagg gttttccccg
    32521 tttcccttac cttataagcc agaccgaaat cgtccagccc ccggacggca aaggacggac
    32581 ggcccaggat cttccgtatc tcacggacct tgtgacggtt ttgcgtggcg atgaccacga
    32641 gggctgaaaa tttcgtcatt cgacggggat tgcttttcta aaaagagatc ccgccggcgg
    32701 cgcggggcgg cggggcgatg gaccggagga cttttttctg caggttgaaa acttttttca
    32761 accctttgcg gcccgcgttg agcagccggt ccagctcctg ccgtgagaaa acgcggcctt
    32821 cggcgctccc ctggatctcc acgaaacggc cggacccggt catcaccatg ttcaagtcca
    32881 cgtcggcgtc gtggtcagcc tcgtaggaca tatccagctc gatcttgccc ttgcaaagac
    32941 cgacgctcac cgcggccagg taatccttca cgggccaggt ggtgatgcgg ccgtcgcggt
    33001 aaagtttccg gagcgcgtcc accaggacga tgaacccgcc gttgatggag gcggtgcggg
    33061 tgccgccgtc ggcctgaagc acgtcgcagt cgaccaccac gctgaacggc gaaagggcct
    33121 gcaggtccac cgcggcgcgc agggagcggc cgatgaggcg ggaaatctct tgggcgcggc
    33181 cgccgccggc ggcccggccg cggggcgtgc gcttgtcgcc ggcgcgggga agcatgccgt
    33241 attccgccga cagccaaccg atcccctttt cctcggcgtg cggcggcagc cgcgtctcca
    33301 cggtggccac gcacaacacg cgggtgtttc ccatctgaaa cagacaggac ccctcggcgt
    33361 gccgaaggta gggccggacg atcttcaagg gacgcatgga agaggacatg tcacgccacc
    33421 gaagaactgg aggacggccg ctgcatccgg ttctctctct gacggaaata gtcgcgcagg
    33481 gactgcaggt gggagacgtg tttctgcacg gcggccagga aatccaccgc cgccgtttcc
    33541 tggcggtgca ggtcctcccc cgcctcttcc agcgcatgaa acgccttctg catggcctcg
    33601 tggtgggcgg ccgacttttc ctgggacgcc ctcccatact cctccccttc gcgggccagg
    33661 cgggccttct ccgcctcgaa ccggtcggat aaatccttga gctgctgttc cagcgcctcc
    33721 gggcccagcg ccttttccag cccggcgcgc ttcgcttcca gggactgccg ctgcctctgc
    33781 agttcctcca gctgcgcgga ccaggcgcgg gcctcctgga aatgtccctg ccgcaccacc
    33841 tccaacgccc cctccgcctc gacgaaaagc cgttccagtt ccgtgcggcg gcggtccagc
    33901 accttctcca gccgggcgcg gcggttggcc acgcgttttt ccaaggactc caaagccagt
    33961 ttttcctggg catgccgggc ctcgccggtg actttttcct ggctggcctt ctgttccata
    34021 acgaggagca gacgttcgta tcggtcgcgc tgctggcgca gccggcgggc gctgtcctgg
    34081 cggaacgccg tgagtttctt ctccagcgac gacagcgtcc tctccatctc cgaaagttcg
    34141 gcgcggcgct tttcctcgtc cgccttcaac cgggagaggg cctcggcgtg ggactgttcg
    34201 gcgctctgcc gttgcgcgcg gagttcggcc tggcggacct gggaggccgc ggtctggtcg
    34261 gagagcgtct cctggtaacg gcgccgttcc ttttccaggg aagaggccag gaccgatttt
    34321 tcttcgcgca atttctccag ccgggccgac aactctttcc gcgtgttctc caagagggcg
    34381 ttcagggtgc ggaaccgctg ttcggcccag cggcgctggt cctcccgccg gagggcatac
    34441 tcccgcgtct gaccgtcgag ttcggcgcgc agccggccgt gttcctcctg cagttccttg
    34501 atgtgcccgg aggtcgcggc gaggcgctcc tccagggctc gacgctgttc cgcggccatg
    34561 ttttcgaact gggatttgtt cgcggtccac tccgccgttt ccttgttcag caaggcgcgc
    34621 tgggtttcca aatccttttc caccgcctgc cgctcgtatt ccatggcctc gcgaaccttc
    34681 tggacctctt ccgcggccag ggtggactgc cgggcggcgg cggattggat ggccgtgagc
    34741 tgctctttcc agcgggcctg ctgctcgcgg gcggcgcgcc gttgggccgc caacgcctcc
    34801 gcaaaacgct ctttttcctc cacctgctgt ttctggacgg cctcgatgtt cccctccagg
    34861 agcgacttct cccgttccaa accctccagg cgtttttccc tctcggcttt ttcctgcagc
    34921 cacacgccct tcacatccgc ctcgaaatgg actttgtcca gctgggcgcg ggcacgctcg
    34981 tcttccagcc gacggaaatt ttcctcggag gcccggcggg ccagatccca ctccgcgcgc
    35041 aatcgggcgg tctcctgttg gagcttctgc tcctcggact tcaggacatt tccgtgagcc
    35101 cccaggcggg cctcccatcc cgtgcgccaa cggaccagcc gggcggacag acggtccatc
    35161 cgctgccggt aacgcatcct ctgtcgatga gccgcctcct cctgcgccgc ggccgcggcc
    35221 ttccgccggg cgttctccgc ctccaattgg cgaagttttt ccagcagcgg gtcggtcccc
    35281 cgctgggact cgatctcctt ttccatctgt tcccgccggt cccgttcccg gaccagttgt
    35341 tcttcctcga ccttcagggc ctcggccttc gccccggcct tttccaacga ctcgcgcaac
    35401 tgggccgatt cccgggccaa actttctttc gagaccgcgg aggcacggtc ggccgagcgt
    35461 tccctctcga gaaatccctc ccgttccttc cgcacgtccg ccaagaattt ttccacctcg
    35521 gccgccacgg gaccgaacga cggcgccggg acggccgccg gagccgggtc gggactcaac
    35581 atcgtttccg cgcggcgggc cacatcctcc acgcgatcct tcagatggga ggaggccgcc
    35641 ccccacagga ctttcgttcc caacgaccgt cgaatctcct ccacgtcggc cttcaagcgc
    35701 tccacccgtt ggttgaattg gcgcacctcc ggcggcacaa cggccgaagg gccttccttt
    35761 tccagttcgg cgatccgcag ctgcagatag ccgtaaaggt cgtccatgcg gaaacggatt
    35821 ttccggaggt ctggggaggg gaccgtcgaa accgggagcg ttcttacggg gacctcgtcc
    35881 tccgaaccgc ctccgccggg caagacgatg gaaatcacgg cggccacggc gaaccagagg
    35941 aggccgaagc ccgccaggcc gacgccgagc atcagcgggt tatggaggag ggcgtttatt
    36001 tctgcgggca tggggcgacg gcgggaaaga acggctcgat catttaaaat ccagaacgac
    36061 gtacgtccgc tgacgcggcg gggtgagggt ggcgaactta tactccggac cgcccacgcg
    36121 caccacgttc ttgccccgca ggaaatcatc ggccccggcg aggtccttca ccttggaagc
    36181 gggggtccga taatgcatgg ggatgacgac ggaccggggt tggatctgct ccaccacgcg
    36241 ggcggcgtcg gcggcgtcga tggtgtagcg gccacccacg ggaacgagca gaacgtcggc
    36301 cccgcgcagc ggcgggatct gcccgtcgtc gagcacgtgc cccaggtcgc ccaaatgcac
    36361 cagtttgaaa ttggggagtt ccaccacaaa aatcccgttg agaccgcgct gggcaccttt
    36421 ttccttgtcg tgataactgg gcacggtgta gatgcgcgca tcgccgatct cgaactgcgc
    36481 ccggttccag tcgttgccgt tgggaccgac ggcatgcaac accgaatagg cgccgacggg
    36541 cagggaaagg ccggcgtggt ctttgtgttc atggctgacg gtgaccacgt ccacggcctg
    36601 cggcgggttc tcatagccga cggaagccgg caccgggtcc aacaggacgc ggacctcgtc
    36661 gggcgtggtg atgaggaaac acgactgccc cagccagcgg atggacacgt cctccgaggc
    36721 ggccgccacg gcggacccgg ccaggacgcc gaccacggcg gcgcccagaa cgcgacggaa
    36781 ggaacggcgg tcagggaaga tcaagggtca tcccttccca ggccgcatgc acgatgagcg
    36841 ggatcttgcg ttcctgcaga ttctgctgat atttcaccag aaactcgtcg atctggcggt
    36901 ccggccgcat ggggtcgtga tggaagaaca ccaactgttt cgcctgcgcc tggacggcca
    36961 tctcgatggc gaactcgtaa aaactgtgcc cccagctcag cttggtcttg tattcgtccg
    37021 tggtgtactg ggcgtcgtgg atgagcacgt ccaccccgcg caggaaatcg cagaggttgt
    37081 ggtcttcccg ttccagctgg atggaccctt tttcgatgtc gtcgtagacg ggcagaaggt
    37141 tgccctgctg tcgcagaagg taccggaaag gttcgttgtc ggtgatgaag gccaccttgt
    37201 ggcggccgtc ttccacccgg tagcccaggg ccagcccggg gtggttcacg taggtgggga
    37261 agacggtcca tttgttcacg gtcacgggcc gcgtggtgag ctggatgaac ttgagttccg
    37321 ccggcatttc ccgcagcgtg acggggaaat attccgggct catctgccct acgaaaatgt
    37381 tctccagttt ctttccggcg ccctcgcacc cgtagatggt gaaggagttg ccgggcacat
    37441 agaccggcat gaagaacgga aaaccctgga tgtggtcgag gtggaaatgg gtgatgaaaa
    37501 gatgcccgtg gatcttcgtc ccccggaacc gtttctgcag gtccatcccg agcggacgga
    37561 tgccggtgcc ggagtcgaag atgaggaggg tgtcgtcgtc gctcatctcc acgcaagagg
    37621 tgttgccgcc gtatttgacg gtgtcttggc cgggagtggc gatggaaccg cgtgtgcccc
    37681 agaagcgtat tttcatggca aatttttcgg taaggtacct tatcggggta ttattctaca
    37741 tcctttgccg ggtcgtcaag acaacggact aaagtcaccg tgaggcgtaa taatagacgc
    37801 cgtcccaggt ctccggcggc ggggatttgg cgagctgttc gatgcgcgaa agatagagtt
    37861 ggctgggccc gtcgccggga caaagttcca acacctgctt gaagagccgc gcggagcgtt
    37921 cccaatcctt gagcagatag aactgccggg cgttctggaa ccgctgaagc atctccgtct
    37981 ggcggtcctt gttctccccc tgctcgcaca actcgtagat gcgcaccgcg gtggaacggc
    38041 ccttcacccg caccaggtcc agttcccgga agacgaaccc ccgccggctc tcccgctggg
    38101 taaactcgct caccaggcaa tggacgctgt atatccgcgt cagccgttcc aggcgggaag
    38161 ccaggttcac cgcatcgccg agcacggtgt agttgagccg tttttccgaa cccatgttgc
    38221 ccgccaccac gggaccggtg ttgaggccga tgccgatggt gacggcgggt tttccttccc
    38281 aggcgcggcg gtagttaaac tccttcagtt tccaaagcat ctccatggcg gtgtcgagcc
    38341 cgttgtccac gtctccctcg tgattcaccg gcgcgccgaa caacgccatc accgagtcgc
    38401 cgatgtattt gtcgatcacg cccttgttct tctccacgat ctggctcatc agcgtcaggt
    38461 agtcg
//
LOCUS       DAOTFD010000006        37770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_733_length_37770_cov_5.030890, whole genome shotgun sequence.
ACCESSION   DAOTFD010000006 DAOTFD010000000
VERSION     DAOTFD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 37770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37770
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_733_length_37770_cov_5.030890"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="P5079_00895"
     CDS             complement(<1..245)
                     /locus_tag="P5079_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY28572.1"
                     /translation="MRTFWQCFVPLFVAVDAIGVLPIYLGLTQGFSREQHRRVILQSM
                     LTATVVALAFLAAGPAVLRFLRITVPDFMVAGGTLLFV"
     gene            complement(270..746)
                     /gene="bfr"
                     /locus_tag="P5079_00900"
     CDS             complement(270..746)
                     /gene="bfr"
                     /locus_tag="P5079_00900"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRY28573.1"
                     /translation="MKGNPKIIDVLNKLLAEELGAINQYMVHSEMYDNWGYEKLHHKI
                     EKNAMDEMKHAEALMQRILFLEGMPAVTKLGPIRIGKAIPEMITNDIGGEATAIKSYN
                     EAVQLAVQLEDNGSRALFEKILQDEEGHIDLLECQRDQIGQMGLENYLPVQTEKKE"
     gene            806..2053
                     /gene="glmS"
                     /locus_tag="P5079_00905"
     CDS             806..2053
                     /gene="glmS"
                     /locus_tag="P5079_00905"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY28574.1"
                     /translation="MRAESRSVPCHEALILLQSPPGAARPKRRSRPRGSESDPEGKNE
                     PRAETIEKQGFPHYMLKEIHEQPETVAAAMRGRLRPAEGTARLEELNPLLAHLKKTGH
                     LTLLSCGTSYYAGLYGRYLFEALTPLTVDVDVASEFRYRKIRFPADTAAVAISQSGET
                     ADTLAAVQEAKRAGTLTLGLVNVERSSIARAVDAAVPMRAGPEVGVASTKCFTSEGVI
                     LLLLALSLGRERGLGPAEGTRILRGLEALPDQLRAVLGQKEKIRALAEKYHKSSDFLF
                     VGRKHNHPLAFEGALKLKEVSYIHAEAYPAGEMKHGPIAMIDADFPSLALAPQDETYD
                     KMLSDIRELKTRGGKVIAVATEGDKKIGTLADDVLFIPPAVELISPLLAAVPLQLFAY
                     YCAVARGCPVDRPRNLTKSVTAE"
     gene            complement(2216..4105)
                     /locus_tag="P5079_00910"
     CDS             complement(2216..4105)
                     /locus_tag="P5079_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY28575.1"
                     /translation="MKPVALVCMPWQLPGYQNVGLSSLKSVLAAKGIRTDIYYFNLEF
                     AEKIGLALYGALAAELRANWVFSVSLFGPCGDGTLDNSWETVKDHPDMRRLVRQLRAS
                     AYPGKKLKELAYQTVPRFMDECHRRVDWSQYGIVGFSSVCSLMAALSLARKIKRTHPA
                     VPLIVGGNGVFGGMGHALLKGADWIDYVADGEAEESFPELVENIASGRPGRPVRGISS
                     RVGGKIHLSERAPRVDMKKLPPPNYDDYFEALEKTTLLKKLPRTLCVETTRGCWWAEK
                     HPCKHCSGYTNHDATRYISKSPEQFEKELLHLSRRYRMRDLFLMDDLMGPVHFERIFP
                     RLEKRGEGFRFRVWLRPDMTRRDFERMARAGVSSVYVGVDGLDGTVLKRINRGVGVSE
                     NVRVLKWCREFGVSVGWNILYGFPDEQRESYAAMRAVLPAISHLSSPTGILPFRLYRD
                     CQYYRYADRFGIGDVKPEAYYALQYPPSRFDLSKLAFVFSFRRRAGAGDSEKRIGPVK
                     RYVVGWRRAFSDKQTWLRYETHPRGIRFDGRRLSSGERPVLRRQVAEYGLLHKEIYQC
                     CEHEPRSAQEIAARVGDGRRPPEAFLGEVESALSEMRTKGFVLEENRRYMGLALPRGS
                     AFCSR"
     gene            complement(4132..4989)
                     /locus_tag="P5079_00915"
     CDS             complement(4132..4989)
                     /locus_tag="P5079_00915"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRY28576.1"
                     /translation="MMAASFQPTFLVIGAAKCGSGYIHEVLCRHPEIAMGQREIHFFS
                     SDKRYKKGWDWYLRQFPDVRGKKAIGEVSHSLSDVKAYPEAVPRILKHVPDARFIYLV
                     RHPLERIESTWMQLRVNPGERDRPVPPFNRAVREVRSLVSASLYWFQISAYRRHFPDD
                     RILVLFLEDIKADPGAALRRCFRFLGVAETVDSAAAAKPVNAAVGKYRDGDFLARARF
                     SLPFRLLRPCVPDFFCRPFYPLIKEKITARPAWDPEVRRWAIEQLREDMRQFLAHYDK
                     PADFWPLIP"
     gene            complement(4998..6479)
                     /locus_tag="P5079_00920"
     CDS             complement(4998..6479)
                     /locus_tag="P5079_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HRY28577.1"
                     /translation="MKTSSRRLKSEEYRNTAALLRLFGGDVRGGRRLTPDRSRRLIAE
                     RDRLLADCLAAGARFHEGNKAKIYSGRLSPGCVACARGTWLCLYLEKRCNRRCFFCPK
                     EKTLSGRVPFENMALTRLDVSGDLETFIRELRYADVSFSGGEPFLEFERLLSYVKRAR
                     TALGKTGHVWVYTNGDFVTASRLRRLTAAGLDEIRINLSARGYDTAPVRLAKRHIRRV
                     TVEIPAVPERLDRVRALLPELAALGVDHLNLHELKVTPYNEQRLARGGYSFARGQKAC
                     SVVESELTALRLLKESLEKKIRLSVNFCSQCYRERFQQRAYQRVLAEWFVRKAPHRGF
                     SVVRETGLLRRVYFENGAERARSPKSGGVLRREGEKCYVRPESLDAPALKARTVFLEY
                     SVCRPTDDPRESAATLEVSGRTFFHVKRVGLRSVPLRRWEERRLFKKMFLGSRSPGEI
                     LNGSSFGGKNERRRAALARAMEKMRSAFDGLETLPRGLPVKYA"
     gene            6569..7372
                     /locus_tag="P5079_00925"
     CDS             6569..7372
                     /locus_tag="P5079_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY28578.1"
                     /translation="MQDDIFLIIRADDFGMCHAVNRAIADAFSGGRITTASVMVPCPW
                     FEEAAALCRRHPRWDVGVHLTLFSEWENYRWGPVLPPSRVSSLVAEDGRFFRSPKELF
                     RRRPSPAEIEREFRAQIERARARGLRLTHLDFHMARTPPLLRIARRLACEFHLPISLD
                     NGENAIRRTFLPSLRSGSAVQKAARALKFLRPGTNIFYCHPGYSHPELAAIRQRHKKP
                     GFLARQRQASFDALAPHALRFLKRGLAVRLIGYREFSLLFPSRRKTSPR"
     gene            7386..8510
                     /locus_tag="P5079_00930"
     CDS             7386..8510
                     /locus_tag="P5079_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY28579.1"
                     /translation="MEKQPPPERPTVLVGDLHYATLRSTLPGLAAQVLKQRPCLILSP
                     RDTVAHDIVAPVPVHYVGLPKKHPWPVVAVSLAVGLWRFFRRHRPALVHLHACALHYY
                     WVWAGVMRILRIPYGVTFHSYVGSHEAGSPAERFRRYLKFREWEFFLLPKALFVSTVS
                     TALLRHLHDRFKKRWDIRVIPNGFDFPSENPPSPTPPLPPTPYLCSLCGGLPGKGADW
                     LLFAFQTVLQTEPNVHLLLCGSGTARLKSLIGLLRLDHRVHLMDFVPRAQVPYLLRNS
                     LFYVHPTRQETFGLAVLEALGEGKAVVAPEIGGLVDYLKNDENAHLVPVLDVAALADG
                     MIRLLKDPAYRSRLEKGARQTADRFTWSQVAPRYLQIYPS"
     gene            8798..9694
                     /locus_tag="P5079_00935"
     CDS             8798..9694
                     /locus_tag="P5079_00935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRY28580.1"
                     /translation="MSFFISVILPCHNRRRFFAQALAGLSQQTFRNFELIIVDDASAP
                     PLLETVRRARLPRWRYLYLGKNRGPAEARNLGIRNAEGDLIALLDSDDVWYPQKLAVQ
                     VRCFADPKIDFVYSNVSEIDGRGRVVLRKVIREGDLDPASGLPKSLPYTSTIVLRKKI
                     LARVGLFDPGFRHYYDDSDFWCRVLKKSSGRIHYLSQALAAYRMPRGKRYSPARYSLS
                     PSLKKESPRIVDYFLDQAYFTIKRRRADQPLPLKYYRHGRNAGVDLPPLPENGRYLPW
                     IRRILENQNEPRCPAPRPRPRP"
     gene            9691..11619
                     /locus_tag="P5079_00940"
     CDS             9691..11619
                     /locus_tag="P5079_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY28581.1"
                     /translation="MTAPRVALVSAPWNCTNGFLDQTPALLKGLLARRGLSANIHPLN
                     LEFIESAGCHLYVALIRWKFSDLVFSGRKIPADDPELRASLENIFAEFPDVSAEKIAE
                     LMEETVPAFLAEVPALADWSLYRLIALHAQKDLMSSLRIAEVLKSANPAGRIFLSGER
                     GQGECGREILAACPWIDYVADGEPEKILPDLAARVVAGQSAEDVRGLLYRGNGGVRTT
                     GRAERLDPNELPPPDYDDFFRRLKRSPVLRRLQRVLPLETSRGCWWAEKHPCFFCGFH
                     PDEETARFLSKKPDRLLEEITHLAERYEELEFQLQDKTLDLDFFKELLPRLKNGDNDF
                     SFSGILRPNVTREQLGLLKEAGFQTIYWGLESLSTELLKRAGKGTTAGQNVRLLKWCR
                     ELDITAFWNFLYGFPGESPDTYAQLLKRLPALSHLPAPSFIQRLELRRGSPHRADPGA
                     FGFADVKPSLLETLIWRPPLFHPEKMAMFFDYSYAEQRGDPDFYVAPLRDFWKRWKTA
                     YREKKTYLFYERGVDFIRFYDNRLTLTGHPNEYLSRVFEYGQLHKAIYLFCENDLREP
                     REILRHIRSLWPDSPDSDILSALHELGQAGLLFEDAGRCVGLALPRASLSRPLIQQAH
                     KNLLKTSVISPALLEDPL"
     gene            11623..13680
                     /locus_tag="P5079_00945"
     CDS             11623..13680
                     /locus_tag="P5079_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY28582.1"
                     /translation="MSPRRLPDRPRSPGTAKPKGRVALVSMPFSNLHIPNVALGLMKT
                     ALQDKGIGAEIFNFHLLFAKRIGLETYRRLSSSFDIQPAWLFSAPLFGSTNVDSLLRR
                     GWKNTEVRKYLLSLNCRPGESEWASLKQLLREDVPQFMTECLRAVDWKKYDVAAFSCA
                     LPYTVPSLVLAKKIKEISRRTRIVLGGPDTYNEMGHALLRGAPWIDFVVNGEGEVAFP
                     LIVANILARRPNTPVPGVSCRRGKKIFLSPLAPPVDIEQSPAPDYSELFHEKNASPVL
                     KNVETALPYAATRGCARANARPCLFCGYRCTDIRKFRLQPPEHVKNALMLLSKRYRGL
                     AFYFADSALPSKYYDELFPLLRQTGVDFTFYGFAHPDIPRKHVRCLKAAGFNSIDFGI
                     ENLHSDLLKNLGKGFTALQAIRLLKWCREDDICVSWNFLCGLPGENAAHYEKNWKLLP
                     HLTHLSPPKIFLPLRLYRDSVHFCRRQDYGFERVRPNPIYRAIYPEKKFILTDVAYAF
                     SYVARWQLPDLEQRMTRLNNWRWEWQKAVWENETFLFYERGPGFIRFYDRRFRPGNPQ
                     EPPQPRIIQRPLLHKNIYLLCADNLPSVDDLLAQLKKADGAAPDRRAVEETLSDLTSL
                     GLVYEEEGRYLGLALPAAAYPDSVRRGTSWERLNFHWKEERPDDALLTERAPGERGAS
                     RSL"
     gene            complement(13689..15605)
                     /locus_tag="P5079_00950"
     CDS             complement(13689..15605)
                     /locus_tag="P5079_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY28583.1"
                     /translation="MRKKSSKTARRPIRVALVVPPWHSVVMDSMGPVVLKNYLEAHGV
                     GTDIHYLNLSLARRVGLESYHDAAYTMAGEALFAPFLPGGSAGKRTVAVPEAYRWVES
                     APDFARTCRRMAEQIPTYLDDCLRDVDWGRYGVIGFTVWSTYTVPALVLSSRIKGKFP
                     QARVVFGGPGVASPVGPRLLREFPWLDAVVQGDGEEPLLEWVSRPSAPRPVAGVWERV
                     GGKIFPPPAPARPVGLKELPAALLRDDYVKGLKKSGLGAFVDPIFQLEGGRGCWYGQH
                     TQCAFCGIDRDAIAYRRRSADSVWKEMRGLMRKYGVRKFRFTDNNISPAHIVQLLPRL
                     RGLKDRPQVFWCMKVTKQKEHIRSMASAGVRFVQIGIENMSRRILDMMQKGTTPLDNA
                     QVLKWCRESGPVALWNFLYGVPGELPEDYRESLRWLGRLSHCQPPTVHPIRLERFSPF
                     FERARRYRLRRLRPDPIYRVLYPGRSDREGLAFFFDADFPGKSERDRRSAPLRRFCEA
                     WRRCFPTKVFCRVERRPGGLRIRDQRLWPTAKGPRLKRKTHRLDGWKADLYSFCDGAR
                     SFVELRRWAEARGVPQKQLSAALADMKKREIIVDEEGRWLALAVDETLRLPVPADPGT
                     ARLFSETARPAVHP"
     gene            complement(15834..16334)
                     /locus_tag="P5079_00955"
     CDS             complement(15834..16334)
                     /locus_tag="P5079_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28584.1"
                     /translation="MSADSLDVLLGRQAKDLVLAMTFAVKDLRGSYARHPVEEIYAHN
                     EIYFSAHRQNLLRGNMQPLIDFADMITRKRALDRFRLHELLRAAFGFKKAVYPLLLEH
                     FWDHRDELVKSLLLVDRSVDRFVVNLAQAFVHYAKEDLARGPLEFPVWLNARPFQSER
                     ELVEDA"
     gene            16677..17363
                     /locus_tag="P5079_00960"
     CDS             16677..17363
                     /locus_tag="P5079_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY28585.1"
                     /translation="MAYRVLIVDDDAAIVKMLEMWLRERGYLTAHCADGAEVPAKAVA
                     WKPDVILLDVLLRGADGLEVCARLKRNPATKCIPVIMMSGFRKQMEDAVTGLRGGAVD
                     YLVKPFSADLLAAKLEILLRRCHHAEPEVVRERHGLKLYSNERRVTVGDKEIRLTRKE
                     FDLLDRLMGNPFRILSPQSLLETVWGYDADAYTDPHTVEVHMSRLRRKLGKKFSSHIV
                     NLIGSGYRLD"
     gene            17463..18956
                     /locus_tag="P5079_00965"
     CDS             17463..18956
                     /locus_tag="P5079_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY28586.1"
                     /translation="MRVTLVNCPGWVRRLPPLGAALLKAFANSQGHEATCLDLSNNLY
                     HATSEKYKPFWNTEHMHYFWMDEGRVRAFIDENEGAVDREVGKILDLQPHVVGFSLYI
                     NNLSMTLEVLKRVKKRAPSVFIVVGGPEVCSKAKFLLEHREIDAAVPGEGENALAGLL
                     QTLQESGPRNNLPGAYVRADNPQGYVFTPVPVVEHLDELPFPDFDDFALDTYALPGHM
                     PTYFGRGCFKKCVFCDEAFLWKKWRTRSGRRTFEELRHLKRRHPSVALFEFYDSLLNS
                     DLRALEEFCDAILQDDSWNRPENRFQWLGNAIVRYDMGRPLLEKMAKAGCYLLQLGIE
                     SGSQRVVTSMKKGFRVPDADRVLADLHAVGIKAMVNIMVGFPTETEEDFQQTLDFLRR
                     NAPAIDRVAPNGPTGIVENTFLHKNAENIYGVDPGSLHPEFWSTRDGQNTYPVRLDRF
                     QRLCDFAQKLNLKLGAPHRQWEFWREEKLKRYADFKQQRSQPQKTSD"
     gene            complement(18966..20105)
                     /locus_tag="P5079_00970"
     CDS             complement(18966..20105)
                     /locus_tag="P5079_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRY28587.1"
                     /translation="MKGLTHFLSGIAAATFIPEVIRMSTQSRVDTVEGAANSFIMLLA
                     GAYGLLPDTLDFKVGQYFSAAEYEIDPDPRNPDPQAMADKFAAAIAHAGRTGEFTRVQ
                     FYPTQLGASLWRQYAVLFPKKEVIIQFNEMVKTSQCPIPGTAPKQNRVGRAPLEYELK
                     ARNLEIDWLNKAVRFLRRLIKGPDKEAAVKPSTVEIFSGAQFGFQLEKDGKLYFNWLP
                     WHRTWSHSYVLGFLLTLPIHLIAYRMGLFHWWLYGLVAFLGFFTHITEDMTGHIGGSL
                     FWPLHRPRSEGLELFKASDPRTNFSIMYAAIVLIVWNTDRFSSKLIPLSGAEVLTYFL
                     VIPLAVYFYAAAKIKGWIRTKEEKALAAIYDTEEDADGNAESVMD"
     gene            complement(20106..21548)
                     /locus_tag="P5079_00975"
     CDS             complement(20106..21548)
                     /locus_tag="P5079_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRY28588.1"
                     /translation="MIELTVIAYCLLGTVLGGLLCLIPSLHIYNVTGIAILIYLKFRS
                     VIPEYALVPFFMAMMVAFAFLNTIPMTFFSAADESAGASILPSMDMLKNGKGRDASLL
                     AGAGTLLGVLLLLPLTPFFYKVCTYIYAISRLHLHWIIGLILVHYVMSEWPKGAGRGR
                     KPWEKFKNGWSNIFAGLLTFALASIVGLILTTKPLSSPELSFQSIMPIFVGFFAFPSI
                     LQTMLSKAKYPLVQYESKYLNAGWGDFGYACLPGVVGGLMTALIPAVTIGIAAISASH
                     MTNHRNLRQTSFEKPIEEGTVVHVHTPEIYFVQERIFLIAGGINKIIYYVGAFLLLFV
                     LTDVTPNGMGRGGMNIMLKPLTTPQPGDYFIMLGTILFSAGISMLMLMWFTKLTVKHF
                     KKLTDNLKSIYAVITVFIIGIVYFMGGGLHDVSGGLTALMVAAVTTCIGSIPVFFNCR
                     RSHCMAVLLVPIAVNMAGYGDTVAKWLGLL"
     gene            complement(21566..22966)
                     /locus_tag="P5079_00980"
     CDS             complement(21566..22966)
                     /locus_tag="P5079_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28589.1"
                     /translation="MTDNRQENSAPPETQAVIVDEEGLCPTCGYFAGSLLSCPRCGAR
                     VEKRIAVKIVKIASLAGSVLGVLLLWYAAHVKQPKQIHVEDIDERMNGAYIKVVGKVA
                     SYVEDVPRNTLKMTIEDGTGKIGVMAFNKLSQFKKIHKDNLPGLGDLVEVAGSVSETQ
                     KFGIGLFLPVPERVRILKKLEPKKYNIADIKTESIGEIVEIRVFVSSYEEKKTKKGSV
                     FYVFMLSDETGSIGMTVFDSVMEKMPEETRKMFLEKGTELDMTVMITEYHGAPQISIF
                     DFAKIAKVGTVDIDAFQEKLTAKRLASVKPNKFKKLRKKDLGESYIFKGTVKGLRKMG
                     KGVVVTLDDGSDDMSVVVWDTLADKIKGYKNLKEDAVMSGIFQIGEYRDDLQLKIAHP
                     EDVKIEPGKSTAASLEIIQDDDAGAGKGAAGEEEVFREEGAGAASDIGESPLEITQDE
                     ELTIEQEDRPKPKKKK"
     gene            complement(23041..24012)
                     /locus_tag="P5079_00985"
     CDS             complement(23041..24012)
                     /locus_tag="P5079_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28590.1"
                     /translation="MAAPAGFRVLPMNATKFEEQIFCLWNLADRCNFGCPYCGYVGEW
                     VPEPAELSAAEWIGFWDRAHSLYGPMNIGVAGKVEPFLTKDFFEILRSVSEKHTLSIT
                     TNLSWEPDEWLPALRPGRVRLHASFHPYFISHDTLLQKILSLKARGIGVMTSMVAYPP
                     LLNDIRTYYDGFRRHGVLCWIRPFHGKYQGKQYPAAHSPEEKELIRMGGDDKAVRYQI
                     WDIRTKDKMCRAGQKGFVLAADGSLRRCPNANEPFGNAKDPTFRLLDAPRPCPVDRCT
                     CAALAPCLVENDAQRLRDDRYVRPVGAAGKKAPALVRRDCHSRENAP"
     gene            complement(24111..27182)
                     /locus_tag="P5079_00990"
     CDS             complement(24111..27182)
                     /locus_tag="P5079_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY28591.1"
                     /translation="MKLVDVCIKRPVFTTMMVSALVVLGAVSYTRLGLDQFPNVDFPF
                     AIVSTTLAGASPEEIETSISKPIEEAINSISGIELLKSVSYEGLSQVLIQFVLEKDPD
                     VAAQEVRDAVNRVSKDLPEGTDPPVVQKFDPDALPVVSIAVSGRYPMQQLTEITKNKV
                     KEPLETVAGVGKITIVGGREREIHVVVNPAALKAFNLSIYHVKAALKQQNVEIPGGRV
                     DQDSRELILRTLGRVGSPKDFEKVIVANANGVPVRIRDVARVLDTEQEARSMARLDGR
                     TTISLIVQKQSGTNTVQVIQTVKERLGDLKAALPPGVTAEVINDQSSFIIDSVRTVEE
                     HIVLGALLAGLAVLLFMGDWRSTFIAAMSIPTSIIATFILIQAAGFTLNMMTLLALAL
                     AVGIVIDDSIVVLENIHRYMEHKRIRPEEAASKATAEIGLAVMATTMSLIVIFLPLAY
                     MSGIVGRFLKSYGLTVAFAIAVSLFIAFTLTPMLCAKFLRLSHGPRNALQIWVDRFND
                     VLRDHYGRLVVWSLGHRMWIVGVSAGIVLSSGVFLAIIGKDFLPSDDTGDFNVEIRAP
                     EGTSLSTTDDILKQIEADIRRLPAVRSLLSSIGEQEGAGVNQGKVYVRLVDYKERRHT
                     QAEIMALTRRALSKYTGLTISVVPAGGFHGGGKEADFEYTLTGPDISKLKDYSDRFIQ
                     ELKKVPGLVDFDTSLILKKPELQVQIHRDRAQDLGVQVQDIAMALRAMVSGEEDITKY
                     KEGDELYQVRVRVDKESRKDAESIGGLFIPSSKVGLVRLDNVATLTEAEGPSQIDRLN
                     RQRQVTIYANLEGAALGDALSASNRIARELKMPPEYKTETVGKTKEMNRMLAGFVMAF
                     VLSFIFMYMILASQFESFLHPVTIMLSLPLSIPFALFSLWLSGQPLTIFSIMGVFMLF
                     GIVKKNAILQVDYTNTLRAQGMERDHAILEANKTRLRPILMTTVVLVAAMLPVALGKG
                     PGSANRATMAVVIIGGQTLCLLITLLITPVAYSLFDDAAAWFRRRFSPKPH"
     gene            complement(27293..28186)
                     /locus_tag="P5079_00995"
     CDS             complement(27293..28186)
                     /locus_tag="P5079_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY28592.1"
                     /translation="MKSFPRNYFLFTFGVLLLAGCGPREDGGPKLEAFPVRVEKLTRQ
                     NLEDALSVVGSLKAKNEATLYSRVPGKLLKNVLKEGEAVKKGQTVALVERDEVGVEFK
                     PAPVPSTLDGVVGRTYLDEGADVKTDTPVALVLDTSELIARAEVPERYAGRVRVGQAV
                     RIGLESLPDRSFIARVSRVSPAVDPVTRSLPLEARLENSGGLLKSGMFVQVSVVTEHR
                     AGVLSVPAEALVNGSGPAVFVFENGKAVKREVEVGLRTENRVEVRKGLNEGDQVITFG
                     LFALKDGSPVELIEGTSSPVE"
     gene            complement(28183..29505)
                     /locus_tag="P5079_01000"
     CDS             complement(28183..29505)
                     /locus_tag="P5079_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28593.1"
                     /translation="MRRGLLFLLSATLAWPAAAGSEEPYRLTLLQARELAAKQNIDVA
                     LAEYDLERFDSLYHEVIGSAFPSIDLTGTYTRSFKQSVIFFGGNAIKIGEKNAASATV
                     GATQPLYTGGKVSKAIAAAHQARRAQEAALRGVRDEVDLAVVRLFYAVLLARDIVAIQ
                     KENLDSAQRHYDTIRARYNQGLDSDLVLRRQEVEVANAKTLLISADNMLETARLNLKD
                     VLSLDIDRPLEAEGAFDPVGQAPPENAVLEKWAMSHRPEVLAVRSNTAVARSYVSIAA
                     ASYKPTLDAFANYQWTAQSADKHLDPGEDAESVNGGLTLKFNLFQGGQSWQKVRQAKI
                     DHEKARLVQEKTERAVRREVQEKWLAVREAAARVKAQETAVEQARRALEATEVRYREG
                     DSGQLELTDATFAFNRARLQFAEATHDYWVNIAALERAVGTNLKEVQP"
     gene            complement(29502..30113)
                     /locus_tag="P5079_01005"
     CDS             complement(29502..30113)
                     /locus_tag="P5079_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY28594.1"
                     /translation="MTIQRIDNPEIRKKQILLAAEAALGEKGFHNILLDDVARYANVS
                     KGTLYLYFQDKDDLFDALFQSIASDINARMASLPDLGTLSPIQQLRRITEEQLVFFRE
                     HKEFFSQISLTKPEIYGKRTGGALRLHFAKYVRFLAEKIRAAVQSGELREHDPNLGAL
                     MLMALFRMFFLEASLLEPGVSAPLTLETAWDFFLRGVGREKRP"
     gene            30403..31425
                     /locus_tag="P5079_01010"
     CDS             30403..31425
                     /locus_tag="P5079_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY28595.1"
                     /translation="MKIISVSSEFPTQAETKLITISRVRDAQCPLRYYKNFLEKPRQE
                     PLFIPIELGMGGFFHDYLEKHLKLVMNLHTEIDKHHRIDVNDMLRSFRMSFVWEGKLR
                     PPYKIVLEKLDVEDYVSKLRGVGQNFNDFLMEELPGKRILGVEEPIQLRTEKATLYGK
                     YDLITEDSDGSITLWDWKTNILPGSQSHEEFRNQKIQLGMYAIWLKYKYDLDKVRATA
                     VFFGDKTEQVSEMFSDEVERDVLTFLTAWRDKLNKLDSYPPLPNKSCPWCEWYPVCPA
                     FATGRKPAPAVREGAASPSPSAAPLPFAPRRPEHEEHLLALVLGVVLALALAVAMFFP
                     FLQKLF"
     gene            complement(31427..31747)
                     /locus_tag="P5079_01015"
     CDS             complement(31427..31747)
                     /locus_tag="P5079_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28596.1"
                     /translation="MRIVYLIGLAAFLAGCVTVGKPFPVGLVPAIEVGKTTQQALLRD
                     FGEPARTGIDDGDETWTYMHYKVRLFGSQDTRDLYVRFHKDGTVKSYSFNSSLEEDRG
                     LGRK"
     gene            31813..32709
                     /locus_tag="P5079_01020"
     CDS             31813..32709
                     /locus_tag="P5079_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY28597.1"
                     /translation="MGPRAEALPLLVLHYDQAAQTARCLASILQAGYAPEQIYCLDNG
                     SRTDPAEPLRRRYPRVQFRRIEKNRGFSGGVNEALRWAFDEGFSSAFFLSNDTVLHRD
                     APEKCRETAATAGAGMVAPRVLSLKSPATTESIGGGFDPAAAALRHHRAPGLPPLLGE
                     NDYVPGAAFWLSRDTFLSLNGMDESYHTYWEDVDFSFRARRRGILLARCYDARVGHAI
                     GKTCHKNPLYTAYYYQRNRIRFCEKFLTPKQRETATTAIRDDLEKLARRWGQKNDAER
                     LNYLPALRRELAGERSPAPFLS"
     gene            complement(32646..34796)
                     /locus_tag="P5079_01025"
     CDS             complement(32646..34796)
                     /locus_tag="P5079_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28598.1"
                     /translation="MNEMIGRRPVDGSWPFRRWRRTVPAAALLLLTLAAYGPAYRAGF
                     VWDDDNFVMQNASLRTGEGLRRIWTEYGAVPQAYPLLYTAFWVQHKLWGFSPAGYHVV
                     NVALHGANAVLAWNVLAALSVPWAWWAAALFAVHPVHAESVAWVTELKNVLSGFFYLG
                     AFLCYLKFEKSRQGAPPGRGRGSCYAAALGLFLCALMSKSVTASFPAAMALTIWWQRG
                     RLRWREVSPLAPFLALGVLAGFLSSTMERRFVGAMGGEWDLAFLERILIAGRALWFYA
                     GKLAWPSGLTFIYPRWTIEMTAWGHVLFPAAVIAVFAALWIQRQKIGRGPLAAVLFFA
                     GTLAPALGFFDFYPMRFSFVADHFQYLASLGLLGLGAGVVGSRIKKGKIAVPVAAALL
                     LVLAGRTWTETGKFRDAETLWRDTLQKNPQSWLAHNNLGNLLVAENRIPAAIRSFRRA
                     LILRPGYPEARYNLGLAYFKKGEYEKALEQYDALLRLRPRFAAGYANRANFLWALNRR
                     SEAWRDYETALRLDPGLPEAHLNAANAFAEIGETEKAEAHYVAALNSRSDSAETHYNF
                     AVMLRRAGRAPEAEKHLRAAVALRPDFSAAPAALGSLLLQRGALSESLVHLRRALALD
                     PRREKSWVEAGIVLGRLGDFEGALKHFDAAVRLDPGDAFAQANRGMVLERLGRGRAAE
                     ESFKTAVALDPSLGYDKKGAGLRSPANSRRSAGR"
     gene            complement(34808..35674)
                     /locus_tag="P5079_01030"
     CDS             complement(34808..35674)
                     /locus_tag="P5079_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRY28599.1"
                     /translation="MKRPSPGWIGIAAAAVLFLWSSPAQARPMKPIATAQAFEEAWNT
                     QDPERVSGFFGAEAVLTMAEGGERYVGKEGMRDFLSTHLRGYRSTSRDFKADGEVVSW
                     VAEMASDHYRALGIEKVLLSKKAVVRDGAVASLELAVSAESRLRIRAAVEERNKERVR
                     RLVEELFNRHNIGVVPQYVSPRYVDHHPPRGVAPDYGGVTANVAGLFNAFPEATVVLD
                     DVLADGDKVMVRARLTGVQRGPWMGRPATGRRLEIESAHVVRLIDGQIVEHWGLADPT
                     PLLKQSPPKKAP"
     gene            35829..37625
                     /locus_tag="P5079_01035"
     CDS             35829..37625
                     /locus_tag="P5079_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28600.1"
                     /translation="MPTARPIHTPHPDVPPSEKISPAEGLLSGSLARGLEISLFLLML
                     SFFLASVFYRFDSPGMIRDQLLDEVRGHHMAQQLRAGQPLLETLDIPFPLYHGSLISY
                     LMMPFFYFVGPSWTLVRAWAVGAGLLALILSHLFLRRAFSPAVALLTLFLLVVHPPFI
                     MGIKMVNYTAPTLLVFSLGALLLFQNGWRTRNPLYFFLGTACLSGGIGMRLWFAWFIG
                     ALAGSALVFSGPIRRHFDFPRSRNLLLSLGLGGLAFLPWLSLTLRTELSGRWDSFSLL
                     SWVYLRPLSILSRASHAASADALWDPLFRAAAEVFHSFNANFFQGRFYFSYIFSSDAP
                     VTNTYYPFLFWTCLALLLFLLLQPKNFPLRPQAGRLVVFFILLYLQLFTLYKEPLHHT
                     YLFYPFPQLIVVVALSSLFALVRRRTPLLCLLLPFLGLFLIQEFRSLRNYYVHIRDFG
                     GRGPATDSVYALATWMEKNLSPKETVLVSEEFLGEHLYFLCPDFKTSPPLFPFRVMDD
                     AHIKLSITSMWQNTIKTYGAFIFINGVYVKRSTYFLPIDLFDHVPWSAKDAPELLASF
                     RDRDGHPTFDVYRFKETAHDRKPLRRPPPRRL"
     gene            37582..>37770
                     /locus_tag="P5079_01040"
     CDS             37582..>37770
                     /locus_tag="P5079_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRY28601.1"
                     /translation="MTENPSDDPLHVVYDDPRLIAVNKAAGQLVVPGRGETGEPLNAR
                     LEKLIGRKIYVVHRLDREA"
BASE COUNT         7294 a        12299 c        10676 g         7501 t
ORIGIN      
        1 acaaagagca gcgtgccgcc cgccaccatg aagtccggca cggtgatcct taaaaaccgc
       61 agaaccgccg ggccggccgc cagaaacgcc agcgccacca ccgtggcggt gagcatcgac
      121 tgcaggatca cccgtcggtg ctgctcccgc gaaaagccct gcgtcaggcc cagatagatg
      181 ggcaagacgc cgatggcgtc caccgccacg aagagcggca cgaagcactg ccaaaaggtc
      241 cgcacggaaa agtcctcccg gaaccccggt tattcctttt tttcggtctg gaccggcagg
      301 tagttctcca gccccatctg gccgatctga tcgcgctggc attccagcag atcgatgtgg
      361 ccttcctcgt cctgcaggat cttttcgaag agcgcccggg acccgttgtc ctccaactgc
      421 accgcaagct ggacggcctc gttgtaggac ttgatggcgg tggcttcgcc gccgatgtcg
      481 ttggtgatca tctccgggat ggccttgccg atgcgtatcg ggccgagctt ggtgacggcc
      541 ggcatgcctt ccaggaaaag gatccgctgc atgagcgcct cggcgtgttt catctcgtcc
      601 atggcgtttt tctcgatctt gtgatggagt ttctcgtagc cccagttgtc gtacatctcc
      661 gaatgcacca tgtactggtt gatggccccg agctcttccg ccaaaagctt gttcagcacg
      721 tcgatgatct tgggatttcc cttcataacg acctccttga gtccgttgtc gagccgttat
      781 tctactaaat aatgccgccg gctttttgcg cgcggaatcg cggtccgttc cgtgccatga
      841 agcgctaatt ttattacaat cccctccagg cgcggcacgg ccgaagaggc gaagccgccc
      901 gcgcgggtcc gagtccgatc ccgaaggaaa aaacgaaccg agggctgaaa cgatagagaa
      961 acagggattc ccccattata tgctcaagga gatccacgag cagccggaga ccgtcgccgc
     1021 cgcgatgcgc ggacgccttc ggcccgcgga gggaaccgcc cggttggagg aattgaaccc
     1081 gctcctggcg cacctgaaaa aaaccggcca cctcaccctg ctctcctgcg gcaccagcta
     1141 ctacgccggc ctgtacggcc gctatctctt tgaagcgctc accccgctga cggtggacgt
     1201 ggatgtggcg tccgagttcc gctaccgaaa gatccgtttc ccggcggaca cggcggccgt
     1261 ggccatcagc cagtcgggcg aaaccgcgga caccctggcc gcggttcagg aggcgaaacg
     1321 cgcgggaacg ctgacgctgg ggctggtgaa cgtcgagaga tcgtccatcg cccgggcggt
     1381 ggacgccgcc gtccccatgc gcgccgggcc ggaagtcggc gtggcctcca ccaaatgttt
     1441 tacgtcggaa ggagtgatcc tgctcctcct ggccctgtcg ttggggcgcg aacgcgggct
     1501 ggggccggcg gaagggacgc ggatcctccg cgggctggag gccctgccgg accagctgcg
     1561 cgccgttctg ggacagaaag aaaaaatccg cgcgctggcg gaaaaatatc acaaaagttc
     1621 cgatttcctc ttcgtcggcc ggaagcacaa ccaccccctc gccttcgagg gagcgctgaa
     1681 actgaaagaa gtctcctaca tccacgccga ggcctatccc gccggtgaaa tgaagcacgg
     1741 ccccatcgcc atgatcgacg ccgatttccc ctccctggct ctggcgcctc aggacgagac
     1801 ctacgacaag atgctctcgg acatccggga actcaaaacg cggggaggga aggtgatcgc
     1861 ggtggccacg gagggcgaca agaaaatcgg gactctggcg gacgacgtcc tcttcatccc
     1921 gccggcggtc gaactgattt caccgcttct ggcggccgtt ccgctgcagc tcttcgcgta
     1981 ttattgcgcg gtggcgcggg gctgcccggt ggaccgcccg cgcaacctca ccaaaagcgt
     2041 gacggcggaa tgacgcaccg ccccccatgg ggggcggcat ctcctcttct gaataaccgt
     2101 ttttgttctc gacttcgctc gaacaatccc ccgcgttact tttcgaacga gcagcgcgag
     2161 cggggggcgg tattcggaaa ggacggggaa taaccgtctt cacccggggc gccgctcaac
     2221 gggagcagaa ggcgctcccc cggggcagcg ccaaacccat atatcgcctg ttctcctcca
     2281 acacaaaccc ttttgtccgc atttccgaca aagcgctttc cacttcccca agaaaagcct
     2341 ccggaggacg ccgaccgtcc cccacccgcg cggcgatttc ctgcgccgac cgtggttcgt
     2401 gttcgcaaca ttgatatatc tctttgtgca ggagtccgta ctccgcgacc tggcggcgca
     2461 agacgggccg ctcgccggac gacagccggc ggccgtcgaa ccggatcccg cggggatgcg
     2521 tctcatagcg cagccaggtt tgtttatccg aaaaagcccg tctccagccg acgacatacc
     2581 gtttgaccgg cccgatgcgt ttttccgaat ctcccgcccc cgcccttctc cgaaaactga
     2641 aaacgaacgc cagcttcgac agatcgaacc gcgacggcgg atattgcaac gcatagtatg
     2701 cctcgggctt tacatcgccg atcccgaacc ggtccgcgta gcggtagtat tgacaatccc
     2761 tgtacaatcg aaacggcaaa atgccggtgg gggacgacag gtgggaaatg gccggcagca
     2821 cggcgcgcat cgccgcataa ctttcccgtt gttcgtcggg aaacccatac aggatgttcc
     2881 aacccacgga aacgccgaac tcgcggcacc acttcagaac gcggacgttt tccgacacgc
     2941 cgacgccgcg attgatcctt ttcaggacgg tcccgtccag cccgtccacc cccacataaa
     3001 ccgaactcac cccggcgcgg gccatccgtt caaaatcccg gcgggtcatg tcggggcgga
     3061 gccacacgcg gaacctgaat ccctcgcccc tcttctccaa ccgcgggaaa atgcgctcga
     3121 agtgcacggg ccccatcaga tcgtccatca agaacaggtc ccgcattcgg tatcgacggg
     3181 acaaatggag gagttccttt tcgaattgtt ccgggctttt tgaaatatac cgcgttgcgt
     3241 cgtggttggt gtagccggaa caatgcttgc agggatgctt ttccgcccac cagcagccgc
     3301 gcgtcgtttc gacacacaac gtccgtggaa gctttttcaa gagggtggtt ttctcgaggg
     3361 cttcaaaata atcgtcgtaa ttcggcgggg gaagtttttt catgtccacc cggggcgccc
     3421 gttcgctcaa atggattttc ccgcccacgc ggctggaaat gccccgcacc ggccggccgg
     3481 gacggccgct ggcaatgttc tcaaccagtt cgggaaacga ctcctccgcc tctccatcgg
     3541 ccacatagtc gatccaatcc gctcctttca agagcgcgtg ccccatcccc ccaaaaacac
     3601 cgttgccgcc cacgatcagg gggaccgccg gatgcgtccg cttgatcttc cttgccagcg
     3661 acagcgcggc catcaggctg cagacggagg agaatccgac gatgccgtat tgggaccaat
     3721 ccacccgcct atgacattcg tccatgaacc gggggaccgt ttgataggcc aattccttga
     3781 gttttttccc gggatacgca ctggcgcgta attgacggac gaggcgcctc atgtccggat
     3841 ggtctttgac ggtttcccag gaattatcca aggtcccgtc cccgcacggc ccaaaaagag
     3901 acaccgaaaa aacccaattc gcgcgcaact cggccgccag ggctccgtac agggccaggc
     3961 ctattttctc ggcaaactct aagttgaagt aatagatgtc ggtccgaatg cccttcgcgg
     4021 ccaaaacgct tttcagactg gacaatccca cgttctggta ccccggcaac tgccagggca
     4081 tgcaaaccag ggcgaccggt ttcacggagg ggcggggatc tttcgtcgga atcacgggat
     4141 gagcggccag aaatccgcgg gcttgtcgta atgcgccaga aattggcgca tatcctcccg
     4201 aagctgttcg atggcccacc ggcgcacctc cgggtcccac gccggccggg cggtgatttt
     4261 ctccttgatc agcggataaa acggccggca gaagaaatcc ggaacgcagg gccgcagcaa
     4321 ccggaagggg agagagaacc gggcgcgggc caggaagtcg ccgtccctat acttgccgac
     4381 ggccgcgttc accggttttg ccgccgccgc gctgtccacg gtctccgcga cgcccaggaa
     4441 tcgaaaacat ctcctcaggg ccgcccccgg atccgccttg atgtcctcca aaaagaggac
     4501 cagtatcctg tcgtccggga aatgccgtcg atacgccgag atctggaacc aatacaggct
     4561 ggccgaaacc agcgagcgga cctctcgcac cgcccgattg aacggaggga cgggccggtc
     4621 cctttccccc ggattgacgc ggagctgcat ccacgtggac tcgatccgct ccagcgggtg
     4681 ccgcaccagg tagatgaacc gggcgtccgg aacatgtttc aatatcctgg ggacggcctc
     4741 cggataggcc ttcacatccg acagcgagtg actgacctcc ccgatggcct ttttcccccg
     4801 gacgtccgga aactgccgga gataccaatc ccatcctttt ttgtaccttt tgtcgcttga
     4861 aaaaaaatgg atctccctct gccccatcgc gatctcggga tgccggcaaa ggacctcatg
     4921 gatatacccc gacccgcatt tggcggcgcc aatgaccaga aacgtcggct ggaacgatgc
     4981 ggccatcatc gactccctca ggcgtatttc acgggcaggc cccggggaag ggtttccaac
     5041 ccgtcaaacg cactcctcat tttctccatc gcccgcgcaa gggccgcgcg acggcgttcg
     5101 ttttttcccc cgaaagaaga accgtttaaa atttcgccgg gcgaccgcga tcctaaaaac
     5161 atttttttga acaaccgccg ctcctcccac cgcctcagcg gcacggagcg aagaccgacc
     5221 cgcttcacat ggaaaaacgt ccggcccgaa acctccagcg ttgccgcgct ctcccggggg
     5281 tcgtcggtgg gccggcagac gctgtattcc aggaacaccg tccgcgcctt cagagcgggg
     5341 gcgtccagac tctccggccg gacatagcat ttctcgccct cccggcgcag aacgccgccg
     5401 gatttcggcg aacgggcgcg ctccgcgccg ttttcgaaat agacccttcg gagaagcccg
     5461 gtctcccgaa cgacggagaa cccccgatgc ggcgccttcc ggacgaacca ctcggccaac
     5521 acacgctgat aagcgcgctg ttggaacctc tcgcgataac actgcgaaca aaaattcacc
     5581 gacaggcgga tcttcttttc gagggactct ttcagcagcc gcaacgccgt cagttccgat
     5641 tccacgacgc tgcaggcctt ttgcccccgg gcgaaggaat agcccccccg ggcaaggcgt
     5701 tgctcgttat acggtgttac cttcaattcg tgcaggttga ggtggtccac ccccagggcc
     5761 gccagttccg gcaacagggc ccgcacccgg tcgaggcgtt cggggacggc ggggatctcg
     5821 acggtcacgc ggcggatgtg ccgtttcgcc aggcggaccg gggcggtgtc gtatccccgc
     5881 gcgctgaggt tgatccgaat ctcgtccagt cccgccgcgg tcagccgcct cagccgggac
     5941 gccgtcacga aatccccgtt ggtatagacc cagacatgcc ctgttttgcc gagggcggtc
     6001 cgcgcccttt tcacatagct caacagccgc tcgaattcca ggaacggctc cccgccggaa
     6061 aaactgacgt ccgcgtagcg gagttccctg atgaacgttt cgaggtctcc cgaaacgtcc
     6121 agtctcgtca acgccatgtt ttcgaatgga acgcggccgg aaagggtttt ttctttgggg
     6181 cagaaaaagc accggcggtt gcagcgcttc tcgaggtaca ggcacagcca ggtcccgcgc
     6241 gcgcacgcca cgcatccggg agacagtcgg ccggaatata ttttggcctt gttcccctca
     6301 tggaaccgcg ctccggcggc caggcaatcg gccaacaggc ggtcccgctc ggcgatcaat
     6361 cgcctcgagc ggtcgggcgt caaccgccgc cccccgcgga cgtctccgcc gaaaagacgc
     6421 aaaagcgcgg cggtgttcct gtattcctcc gatttaaggc gcctcgatga ggtcttcatc
     6481 acttcggaaa agatatcgca aaaaagcggg aggcacaaga ggcgcgaaat ccggccgggc
     6541 cgaaacgtct ttttgatata ctttgcgcgt gcaggacgat attttcctca tcatccgcgc
     6601 cgacgatttc ggcatgtgcc acgccgtcaa ccgggccatc gccgacgctt tctccggcgg
     6661 gcggatcacc acggccagcg tcatggtccc gtgcccctgg ttcgaggagg cggcggccct
     6721 ttgccggcgg catccccggt gggacgtggg cgtgcatctc accctcttca gcgagtggga
     6781 gaattatcga tggggccccg ttctgccccc ctcccgagtg tcctcgctgg tggccgaaga
     6841 cggccgattc ttccgctccc cgaaagaact cttccgccgg aggccgagcc ccgccgagat
     6901 cgaacgggaa ttccgcgcgc agatcgagcg ggcccgggcc cgcggcctcc gcctgaccca
     6961 tttggatttc cacatggcgc ggaccccgcc gctcttgagg atcgcgcgac gactcgcctg
     7021 cgaattccat ctccccattt ccctggacaa cggggagaac gccatccgcc gaacttttct
     7081 gccctccctc cgctccggct ctgccgtcca aaaggcggcg cgggccttga aattcctccg
     7141 tccggggaca aacattttct attgtcatcc cggctattcc caccccgaac tggccgcgat
     7201 ccggcaacgc cacaagaagc cgggcttcct ggcccgccag cgccaggcct ccttcgacgc
     7261 cttggccccg cacgcgcttc gcttcctcaa gcgaggactt gccgtccggc tgatcggtta
     7321 tcgggagttc tccctccttt tcccttcgag acgaaaaacg tcccctcgat aggaacaccc
     7381 tctccatgga aaaacagccc ccccccgaac gtccgaccgt gctcgtcgga gacctccatt
     7441 acgccaccct gcggtccacc cttcccggtc tggcggccca ggtgctgaaa caacggccct
     7501 gtctcatttt aagtccgcgc gacaccgtgg cgcacgacat cgttgccccc gtccccgtcc
     7561 attacgtcgg tctgccaaag aaacacccct ggccggtggt ggctgtttcc ctggcggtgg
     7621 ggctttggcg ttttttcaga cgtcatcgcc ccgcgctggt ccatctccat gcctgcgcgt
     7681 tgcactacta ttgggtctgg gccggggtca tgcgaatcct ccgcattccc tacggtgtca
     7741 ccttccacag ctatgtcggt tcccatgagg cgggaagtcc agcggaacgt tttcgtcgct
     7801 atctgaaatt ccgcgaatgg gagttcttcc ttctccccaa agcccttttc gtcagcacgg
     7861 tctcaaccgc cctcctgcgc cacctccacg accgcttcaa gaagagatgg gacatccgcg
     7921 tgatcccgaa cgggttcgac ttcccttcgg aaaatccgcc ctcgcccacc ccgcccctcc
     7981 cgccaacccc gtacctctgt tctctctgcg gagggctgcc cggaaaaggg gcggactggc
     8041 tcctcttcgc ctttcaaacc gtcctccaaa cggagccgaa cgttcatctc ctcctgtgcg
     8101 gttcgggcac ggcgcgtctt aaaagcctga tcggtttgtt gcgactggac caccgggtgc
     8161 atttgatgga ctttgtgccc cgcgcccagg tcccctatct cctacggaac agcctttttt
     8221 acgttcatcc gacccggcag gaaaccttcg gattggcggt tttggaggcc ctcggcgaag
     8281 ggaaagcggt ggtggctcct gaaatcgggg gattggtcga ttatttgaaa aacgatgaaa
     8341 acgcgcattt ggtgcctgtg ttggacgtcg ccgccttggc cgacggcatg attcggctgt
     8401 taaaggaccc ggcctatcgc tcccgattgg aaaaaggcgc ccgccagacg gcggatcgtt
     8461 ttacctggtc ccaagtggcc ccccgctatc ttcaaattta tccctcttaa tttttgttgt
     8521 cattttggcc gttttgtcgc ataatgttaa atgtgcgctt ttgggaaaag gtcagctccc
     8581 aaaaacaatg acgtttggga atcaacggat aacagattat gtctaagaaa aaactcataa
     8641 aaacaagcgg ctccaaacga ggccgtcgtt acgtcaaacc tcatttgacg gtacacggcc
     8701 gcctcgaacg agtagtttac gggatggagt tcactccaga ttaatccttt cggttttctc
     8761 tcccgagcgc gggtgtagcc gttcccgcca caacctcatg tcttttttca tcagcgtcat
     8821 cctcccctgc cacaaccgcc gacggttctt cgcgcaagcg ctcgccggac tttctcaaca
     8881 aacgttcagg aatttcgaac tcatcatcgt ggacgatgcc agcgcgccgc cgcttctgga
     8941 aaccgttcga cgcgcccgcc tgccccgctg gcgatacctc tatctgggga agaaccgcgg
     9001 ccctgccgaa gcccgcaatc tgggcatccg caacgccgag ggggacctga tcgccctgct
     9061 ggattcggac gatgtgtggt atccccaaaa attggccgtc caggtccgct gtttcgccga
     9121 tcccaaaata gatttcgtct attccaacgt ttccgagatc gacggccgtg gccgcgtcgt
     9181 ccttcgcaag gtcatccgcg agggggacct ggaccccgcc tccggcctgc ccaaatccct
     9241 tccctacacc tccaccatcg tcttgcgcaa aaaaatcctg gcgcgggtag ggcttttcga
     9301 tcccgggttt cgccactatt acgacgactc ggatttctgg tgtcgtgtct tgaaaaaatc
     9361 gtccggacgg attcactatc tgtcccaggc cttggccgcc taccgcatgc cccgaggaaa
     9421 aaggtattcc cccgcgcgct attctctgtc cccctcttta aaaaaagaat ctccccgcat
     9481 cgtggactac ttcctcgacc aggcctactt caccattaaa cggcggcgag cggaccagcc
     9541 gctccccttg aaatattacc gccatggccg gaacgcggga gtggatctgc ctcctctccc
     9601 ggaaaacggc cgctatctcc cctggattcg ccgcattctg gaaaatcaaa atgaaccgcg
     9661 ttgtcccgcc ccccggccga ggccgaggcc atgacggctc cccgcgtcgc cctcgtcagc
     9721 gccccctgga actgcaccaa cggttttttg gatcagaccc ccgcccttct taaaggcctc
     9781 ctggcccggc gcgggctctc ggcgaacatc cacccgctga atctggaatt catcgaatcg
     9841 gccggctgtc acctctacgt cgccctcatt cgatggaaat tcagcgacct ggtcttttcc
     9901 ggccgaaaaa ttccggcgga cgatcccgaa ctccgcgcct ccctggaaaa tatttttgcg
     9961 gagttcccgg acgtctccgc cgaaaaaatc gccgaactga tggaagaaac cgtccccgcc
    10021 ttcctggcgg aggtcccggc cctcgcggac tggtccctct accgattgat cgccctgcac
    10081 gcccagaaag acctcatgtc ctctctccgc atcgcggaag tcctcaaatc ggccaatccc
    10141 gcgggccgga tatttctcag cggcgaacgg ggccagggag aatgcgggcg ggagatcctg
    10201 gcggcctgcc cgtggattga ttacgtcgcc gacggcgaac cggaaaaaat acttcccgac
    10261 ctggcggccc gcgtcgtcgc cgggcaatct gccgaagacg tccgcggact gctgtatcgc
    10321 ggaaacggcg gggtgcggac caccgggcgg gcggaacgcc tggacccgaa cgagttgccc
    10381 cctcccgatt acgacgattt tttccggcgg ctcaagcgat cgcccgtgct gcggcgcctg
    10441 caacgggtgc tcccgctgga aacgagccgt ggctgctggt gggccgaaaa acatccctgt
    10501 tttttctgcg ggttccaccc ggacgaagaa accgcccggt tcttgtcaaa aaaaccggac
    10561 cggctcctgg aagaaatcac gcatctggcc gaacgttacg aggaactgga attccagctg
    10621 caggacaaga cgctggacct cgattttttc aaggaactcc tgcctcgtct aaaaaacggc
    10681 gacaacgact tctccttcag cggaatcctg cgccctaacg tcacccgcga acagctcggc
    10741 ctccttaagg aagcgggctt ccagaccatt tattggggac tggaaagtct ctccacggaa
    10801 ctcctgaagc gggcggggaa ggggaccacg gccgggcaga acgtgcgact cctgaaatgg
    10861 tgccgggaac tggacatcac cgccttttgg aatttcctct atggttttcc cggagaatct
    10921 cccgacacct acgcccagct cttgaagcgg ctgccggcgc tgtcgcatct tcccgccccc
    10981 tctttcatcc agcggctcga attacggcgg ggcagccccc accgggccga ccccggcgcg
    11041 ttcggattcg ccgatgtgaa accctccctc ctggaaacgc ttatctggcg ccctcctctc
    11101 tttcatccgg aaaaaatggc gatgttcttc gactactcat acgccgagca acgcggggac
    11161 cccgattttt atgtggctcc ccttcgggat ttctggaaac gctggaaaac cgcctatcgg
    11221 gagaagaaaa cctacctttt ttatgagcgc ggagtggact tcatccggtt ttacgacaac
    11281 cggctcacct tgaccggtca tccgaacgaa tacctctctc gggtctttga atacggacaa
    11341 ttgcataagg cgatctacct cttttgcgaa aacgatctcc gggaaccgcg ggaaatcctc
    11401 cgccatatcc gttcgctttg gccggactcc ccggactcgg acattctttc cgctctgcac
    11461 gaactgggac aagccggact gctgttcgaa gacgcgggcc gttgcgtggg attggccctc
    11521 ccgcgcgcca gcctttcacg cccactgatt cagcaggctc acaaaaatct gctaaagact
    11581 tcggttatct cgccggcgct tctcgaagat ccactttaac ccatgtctcc ccgtcgactc
    11641 cccgaccggc cgcgctcccc cggaaccgcc aaacccaaag gacgcgtcgc cctcgtctcc
    11701 atgccgttca gcaacctgca tattcccaat gtcgccctcg gcctcatgaa aaccgcctta
    11761 caggataagg gcatcggcgc agagattttc aattttcacc tcctgttcgc caagcgcatc
    11821 ggactggaaa cataccgccg cctttccagc agcttcgaca tccagcccgc ttggctcttt
    11881 tccgcccccc tgttcggctc aaccaacgtg gattctctcc ttcgacgtgg atggaaaaac
    11941 acggaggtgc ggaaatatct cctgagcctg aactgccgtc cgggcgaaag cgaatgggcc
    12001 tccttgaaac agctcctccg ggaagacgtt ccccaattca tgaccgaatg tcttcgcgcg
    12061 gtggattgga aaaagtatga cgtggccgct ttttcctgcg ccctccccta tacggtgccg
    12121 tctttggttc tggcgaaaaa aataaaggaa atttcccgtc gaacccggat cgtactgggg
    12181 ggacccgata cctacaacga aatgggccac gcgctccttc ggggggcccc ctggatcgat
    12241 tttgtcgtca acggcgaagg ggaggtggca ttcccgttga tcgtcgccaa catactcgcc
    12301 cgccgaccga acaccccggt cccgggcgtg tcttgccggc gtgggaaaaa aatcttcctt
    12361 tcccccttgg ctccccccgt cgacatcgag caatctccgg cccccgatta ttcggagttg
    12421 tttcatgaaa aaaacgcgtc tcccgttttg aaaaatgtcg agaccgcctt gccctacgcc
    12481 gcaacgcgcg gatgcgcccg ggcgaatgca cgaccctgtc ttttctgtgg ctaccgttgc
    12541 acggacatcc gcaagttccg tcttcagcca cccgagcacg taaagaatgc gctgatgctc
    12601 ctctccaaac gctatcgcgg cctggcattc tatttcgccg acagcgctct tccctcgaaa
    12661 tattacgacg aactcttccc tctcctccgg cagaccgggg tcgacttcac tttttatggt
    12721 tttgcgcacc ccgatattcc tcgaaagcat gtccggtgcc tcaaagccgc cggtttcaac
    12781 tccatcgatt tcggcattga aaatcttcac tccgaccttt tgaaaaacct cggaaaaggc
    12841 ttcacggccc tccaggccat ccgcttgctc aagtggtgcc gggaagacga catctgcgtc
    12901 agctggaatt tcctgtgtgg cctgccggga gaaaatgccg cacattatga aaagaattgg
    12961 aagctcctgc cgcatttgac ccatctatcc ccgccgaaaa tattcctccc cctgcgtctc
    13021 taccgggact ccgtccattt ttgccgtcga caagactacg gattcgagcg ggtccgtccg
    13081 aatccgatct accgcgccat ttatccggag aaaaaattca tcctaacgga cgtcgcttac
    13141 gccttttcct atgtcgctcg atggcaacta ccggacttgg aacagcggat gacccgtctg
    13201 aacaactggc gatgggaatg gcaaaaagcc gtttgggaga atgaaacttt tctcttttac
    13261 gagcgcggcc ccggtttcat ccgcttttac gaccgtcgtt tccggccggg aaaccctcag
    13321 gaaccgcccc aaccacggat catccagcgg ccgctccttc acaagaacat ctatctcctc
    13381 tgtgcggaca acctgccgtc ggtcgacgac ctcctggctc aattgaaaaa agccgacggc
    13441 gccgccccgg accgccgggc ggtggaggaa acgctgtcgg atttgacgtc tctcgggttg
    13501 gtttatgagg aggaaggccg ctacttgggg ttggcgttgc ccgccgccgc ctatcccgac
    13561 agcgtccgac ggggaacaag ctgggaacgg ctcaatttcc actggaaaga agaacgcccc
    13621 gacgacgcct tgctgaccga acgcgcgccg ggagagcggg gggcgtcccg ctctctttaa
    13681 ggaacgtctt acgggtgaac cgccggccga gccgtttccg agaacaaacg cgccgtcccc
    13741 gggtcggcgg gaaccggaag ccgaagggtt tcatcgacgg ccagggccag ccaacggccc
    13801 tcttcgtcaa cgataatttc cctctttttc atgtctgcca acgccgctga aagctgtttt
    13861 tggggcaccc cccgcgcctc ggcccaccgc cgcaattcca cgaacgaccg ggccccgtca
    13921 cagaacgaat acaaatccgc tttccagccg tccagccggt gggttttcct tttcaggcgc
    13981 ggacccttcg ccgtcggcca caatcgctga tcccggatcc gcagtccgcc cggccggcgt
    14041 tccaccctgc aaaatacttt cgtcggaaaa caccggcgcc aggcctcgca gaaacgccgg
    14101 agcggggccg accgccgatc cctctccgac ttccccggaa aatcggcgtc gaagaaaaaa
    14161 gccagcccct ccctgtccga gcggcccgga taaagcaccc ggtatatcgg gtccggacgc
    14221 aggcggcgca ggcggtaacg gcgggcccgt tcgaagaacg gcgaaaagcg ctccaggcgt
    14281 atcggatgca ccgtcggcgg ctggcaatga ctgagccgcc ccagccagcg caggctctcc
    14341 cgataatctt ccggaagttc cccgggcact ccgtaaagaa aattccacaa ggccaccggg
    14401 cccgattccc ggcaccattt gagaacctgg gcgttgtcga gcggcgtcgt gcctttttgc
    14461 atcatatcga ggatccgccg gctcatattt tcgatgccga tctgcacgaa acgtacgccg
    14521 gccgaagcca tgctccgaat gtgctccttc tgtttcgtca ccttcatgca ccaaaacacc
    14581 tgcggcctgt ccttcagccc ccgcagacgg ggcagcagtt gaacgatgtg cgccggtgaa
    14641 atattgttgt ccgtgaaacg gaatttgcgg acgccgtatt tcctcatcaa cccgcgcatc
    14701 tccttccaga cgctgtccgc ggaccgccgc cggtaagcaa tggcgtcccg gtctatcccg
    14761 caaaaagcgc actgcgtatg ctgtccatac caacaccccc gccccccctc cagctggaaa
    14821 atggggtcca cgaacgcccc taatccggac tttttcaatc ccttcacata atcgtcccgc
    14881 aacaacgcgg ccggcagttc cttcagcccc accggccggg caggagccgg gggcgggaag
    14941 attttgccgc cgacgcgttc ccacacgccc gccaccggac gcggcgccga cggccgggaa
    15001 acccattcca acaacggttc ctcgccgtcg ccctgcacca ccgcatcgag ccacggaaat
    15061 tccctcaaca accgggggcc gacaggcgaa gcgaccccgg gccctccaaa aaccacccgc
    15121 gcttgtggaa actttccctt gatccttgaa gacaacacca gggcgggaac cgtataggtg
    15181 ctccaaaccg tgaatccgat gaccccgtaa cggccccagt ccacatctcg aaggcaatcg
    15241 tcgagatacg tcggtatctg ttcggccatg cggcggcacg tcctcgcaaa atccggcgcg
    15301 gactcgaccc atcggtaggc ctccggaacg gcgaccgttc ttttcccggc gcttccgccg
    15361 ggcaaaaacg gcgcgaacaa cgcctccccc gccatggtgt aggccgcatc gtgataggat
    15421 tccaacccca cccggcgggc cagcgacaaa ttcaaatagt ggatatcggt cccgaccccg
    15481 tgcgcctcca gataattttt caggacgacc ggccccatgc tgtccatgac cacgctgtgc
    15541 cacggcggga ccaccaaggc gacccgtatc ggacgacgtg ctgttttgga tgattttttt
    15601 ctcatgcgct tcccgtcaga atttatgcct acgacatgct ccaaccccat gcgttctacc
    15661 attcctacaa ataatgagcc cgccggtcaa ctctcgacgc cccccacggc cgaccgggcg
    15721 gaaaaacagc ggccccggcg caagagcgcg ggccgtcggc gttttttctt ctttgatggg
    15781 gagcgatcgt tatcgggtgt gcgggcgggg gcggggatcc ttggggaaag gggtcaggcg
    15841 tcttcgacca gttccctctc cgattgaaac ggtcgcgcgt tcagccatac ggggaactcc
    15901 aggggtccgc gcgccaggtc ctccttggcg taatggacga acgcctgggc caagttcacg
    15961 acaaaccggt ccacggaccg gtccactaaa agcaaggact tcaccagttc gtcccgatgg
    16021 tcccagaaat gctccaacag gagcgggtag accgcctttt taaaaccgaa cgcggcccga
    16081 agaagctcgt gcaaacggaa ccggtccagg gcgcgcttcc gcgtgatcat gtccgcgaaa
    16141 tcgatcagcg gctgcatgtt gccgcgcaaa aggttctgcc gatgggcgct gaaataaatc
    16201 tcattatggg cgtatatttc ttcgaccggg tggcgggcat aactgccgcg caggtctttg
    16261 acggcgaacg tcatggcgag aacgaggtct tttgcctggc ggcccaacaa cacatcgagg
    16321 gaatcggcgg acattcaaac ctacctctcg gccctggaag aaacattcgg agccgaagac
    16381 atgcggcgca acgatttggt aaaataaaaa ggtgcggggc ctccattcat tacttggcaa
    16441 tttctcacca atggacggtc acatgaaaga gcccttgaat acattccgtc gttcagacgg
    16501 tccgtctggg aatatggatg gcctctttgt cgcttccctc agacgacctc ccgcatgctg
    16561 ccgtgtccca gaggccatcc atattgcccc gctttcctat agtctgccac agttcgttca
    16621 actcccgttc tcttttcgtt taactcttgc tcaaattctc cgtcggggaa tcccctatgg
    16681 cctatcgcgt tctcattgtt gacgacgacg ccgccatcgt aaaaatgctc gagatgtggc
    16741 tgcgggagcg cgggtatctc accgcgcact gcgccgacgg cgccgaggtg ccggccaaag
    16801 cggtcgcctg gaaaccggac gtcatcctcc tggacgtgct cctgcggggc gcggacggcc
    16861 tggaggtttg cgcccggctc aaacgcaatc ccgccaccaa atgcatcccg gtgatcatga
    16921 tgtccgggtt ccggaaacag atggaagacg ccgtgaccgg cctgcgggga ggcgcggtcg
    16981 actatctggt gaaaccgttc tccgccgacc tcctggcggc caaactcgag atcctcctcc
    17041 ggcgatgcca ccacgccgaa ccggaagtgg tccgcgaacg gcacgggctg aaactctatt
    17101 cgaacgaacg ccgggtgacg gtgggggaca aagagatccg gctgacgcgg aaggaattcg
    17161 atctgttgga ccgcctgatg gggaacccgt tccggatatt gtcgccccag tcccttctgg
    17221 aaaccgtttg gggctacgac gcggacgcct acacggaccc gcacaccgtg gaggttcaca
    17281 tgtcgcgcct gcggcggaaa ctggggaaaa aattttcatc gcacatcgtg aacctgatcg
    17341 gctccggcta ccggctggac tgatcccttc ccatcccgac gcccctcgtt cttgaaaaat
    17401 gcggcggcat cttatataac caattgcctg tgtcaaatcc gcgattctga ggggaccttt
    17461 tcatgcgcgt gactctcgtc aattgtccgg gctgggtgcg ccgcctgcca cccctcggcg
    17521 ccgccctgct caaggcgttt gcgaacagtc aagggcacga agcgacctgc ctggacctca
    17581 gcaacaacct ctaccacgcg acctccgaaa aatacaagcc gttctggaac acggaacaca
    17641 tgcactattt ttggatggat gaaggccgcg tccgcgcgtt tatcgacgag aacgaagggg
    17701 ccgtcgaccg ggaggtggga aaaatcctcg acttgcagcc ccacgtcgtc ggcttttccc
    17761 tttacatcaa caacctctcc atgacgctcg aggtattgaa gcgtgtgaaa aagagggccc
    17821 cctcggtgtt catcgtggtg ggcggcccgg aagtctgctc caaggcgaag ttccttctgg
    17881 agcaccggga gatcgacgcg gcggtccccg gcgagggaga gaacgccctg gccggcctcc
    17941 tccaaaccct ccaggaatcc ggcccgcgga acaatctccc gggagcctac gtgcgggcgg
    18001 acaacccgca gggctacgtc ttcacgcccg tccccgtggt cgagcatctg gacgagctgc
    18061 cctttcccga ttttgatgat ttcgccctgg acacgtatgc gctcccgggg cacatgccga
    18121 cctatttcgg acgcggctgt ttcaaaaaat gcgttttctg cgacgaggcc ttcctctgga
    18181 aaaaatggcg gacgcgttcg ggccggcgga cgttcgaaga gctccgccac ctcaaacgcc
    18241 ggcatccctc ggtcgcgctt ttcgaattct acgactcctt gctgaattcg gacctgcgcg
    18301 ccctggagga attttgcgac gccatcctcc aagacgactc ctggaatcga ccggagaacc
    18361 ggttccaatg gctcgggaac gccatcgtcc gctacgacat gggacgccct ttactggaaa
    18421 agatggccaa ggcgggctgc tatctgctgc agctgggcat tgagagcggg tcccagcggg
    18481 tcgtcacctc catgaaaaaa gggttccgcg tgccggacgc ggaccgcgtg ctggcggact
    18541 tgcacgccgt gggcatcaaa gccatggtga acatcatggt ggggtttccg acggaaacgg
    18601 aggaggattt tcagcagacg ctcgacttcc ttcgaaggaa cgctccggcc atcgaccggg
    18661 tggcgccgaa cggccccacg ggcatcgtgg agaacacgtt cctgcataaa aacgccgaaa
    18721 acatttacgg ggtggatccg ggcagcctcc atccggaatt ctggtccacc cgggacgggc
    18781 agaacaccta cccggtgagg ctggaccggt tccagcgatt gtgcgacttc gcccaaaaac
    18841 tcaatcttaa attgggggcc cctcaccggc agtgggaatt ttggcgggaa gaaaagttaa
    18901 aacgttacgc ggatttcaaa cagcagcggt cccaacctca aaaaacctcg gactaatccc
    18961 gccgcctagt ccatgacgga ctcggcgttc ccgtccgcat cctcctcggt gtcgtagatg
    19021 gccgcgaggg ccttttcctc tttggtccgg atccatccct taatcttcgc cgccgcataa
    19081 aagtaaacgg ccagggggat cactaaaaaa tacgtcaaca cctccgcgcc cgacaacggg
    19141 atcagtttgg aggagaaccg gtcggtgttc cagacgatca ggacgatggc cgcatacatg
    19201 atgctgaaat tcgtccgcgg atcgctcgcc ttgaacagtt ccaacccctc ggagcgcggc
    19261 cggtgcagcg gccagaacaa cgacccgccg atatgtcccg tcatgtcttc ggtgatgtgc
    19321 gtgaaaaaac ccaaaaacgc cacgaggccg tagagccacc aatggaagag gcccatccga
    19381 taggcgatga ggtggatcgg gagggtcaaa aggaatccca acacatagga atggctccag
    19441 gtccggtgcc acggcagcca gttgaaatac agcttcccgt ctttttccag ctggaacccg
    19501 aactgcgccc cgctgaatat ctccaccgtg gagggtttca ccgccgcctc cttgtccggc
    19561 cccttgatca accgacggag gaagcggacc gccttgttca accagtcgat ttccagattc
    19621 cgcgccttca actcatattc cagcggggcc ctcccgacgc ggttctgttt cggcgcggtg
    19681 cccgggatgg ggcattggga ggttttcacc atctcgttga actggatgat cacctctttc
    19741 ttcgggaaga ggaccgcgta ttgccgccag aggctggcgc ccagctgggt cggatagaac
    19801 tgcacccgcg tgaattcgcc ggtccggccg gcgtgcgcga tcgccgccgc gaacttgtcg
    19861 gccatggcct gcgggtcggg gttccgcgga tccggatcga tctcgtactc cgcggcggaa
    19921 aaatattgtc ccaccttaaa atccagcgtg tccggcagga gaccataggc ccccgccagc
    19981 agcatgatga acgaattggc cgccccctcc acggtgtcca cccggctctg ggtggacatg
    20041 cggatcactt cggggatgaa ggtcgccgcg gcgatgcccg acaggaaatg cgtgagccct
    20101 ttcatctaca gcaaccccag ccatttggcg accgtgtcgc cgtatcccgc catgttcacc
    20161 gcgatgggca ccagcaggac cgccatgcaa tgcgaccgac ggcagttgaa aaaaaccggg
    20221 atggagccga tgcaggtggt caccgcggcc accatgagag ccgtcagacc gcccgaaacg
    20281 tcgtgcagcc cgccgcccat gaagtagacg atgccgatga tgaagacggt aatgacggcg
    20341 tagatgctct tcagattgtc ggtcagtttt ttgaaatgtt tgacggtcag cttcgtgaac
    20401 cacatcagca tgagcatgga gatgccggca ctgaaaagga tggtccccag catgatgaag
    20461 tagtcgcccg gctggggggt ggtgagcggt ttgagcatga tgttcatgcc gccccgcccc
    20521 atgccgttgg gggtcacgtc ggtcagcacg aagagcagca ggaaagcgcc gacgtagtag
    20581 atgatcttgt tgatcccgcc ggcgatgagg aatatccgct cctgcacgaa atagatctcc
    20641 ggcgtgtgca cgtgcaccac cgtgccctcc tcgatcggct tttcaaacga cgtctgccgg
    20701 aggttccggt ggttggtcat gtggctggcg gagatggcgg cgatgccgat ggtgacggcg
    20761 gggatcaggg cggtcatcag tccgccgacc acgccgggca ggcaggcgta gccgaaatcg
    20821 ccccatccgg cgttgaggta tttcgactcg tactgcacca gggggtattt ggctttcgac
    20881 agcatcgtct gcaagatgga ggggaacgcg aagaagccca cgaagatcgg catgatgctt
    20941 tggaagctca gttccgggga ggacaacggt ttcgtcgtca ggatcagccc cacgatcgag
    21001 gccagcgcga acgtcaggag cccggcgaat atattcgacc atccgttttt gaatttttcc
    21061 cagggcttcc ggccgcgccc cgcgcctttg ggccattcgc tcatgacata atgcaccagg
    21121 atgaggccga tgatccaatg gaggtgcagc cgcgagatcg catagatgta ggtgcacacc
    21181 ttgtagaaaa acggcgttaa cggaaggagc aacagcaccc cgagcagcgt tcccgcgccg
    21241 gccaacaggg aggcgtcgcg tcccttgccg ttcttcagca tgtccatgga cggcaggatg
    21301 gaggccccgg ccgactcgtc cgccgcggag aagaaggtca tggggatggt gttcaggaaa
    21361 gcgaacgcca ccatcatggc catgaagaag gggaccagcg cgtattcggg aatcacggaa
    21421 cggaacttca aatagatgag gatggcgatg cccgtgacgt tgtagatgtg caaggagggg
    21481 atgagacaca aaaggccgcc gaggacggtc ccgaggaggc aataggcgat gacggtgagt
    21541 tcgatcatgg gcgtttttcg aaggactatt ttttcttctt tggtttcggc cggtcctctt
    21601 gttctatcgt caactcctcg tcctgcgtga tttccagcgg gctctccccg atgtccgaag
    21661 cggcccccgc cccttcttcc cggaagacct cctcctcccc ggccgcgcct tttccggccc
    21721 ccgcgtcgtc gtcctgaatg atctccaggg aggcggcggt gcttttgccg ggttcgattt
    21781 tgacgtcttc cggatgggcg attttcagct gaaggtcgtc cctgtattcg ccgatctgga
    21841 atatgccgga catgacggcg tcttccttca ggttcttgta ccccttgatc ttgtccgcca
    21901 gcgtgtccca gaccaccacc gacatgtcgt cggacccgtc gtcgagcgtg acgaccacgc
    21961 ccttccccat cttccggagg cccttcaccg tgcccttgaa gatgtaggat tcgccgaggt
    22021 ccttcttccg tagtttcttg aatttgttgg gcttgacgct cgccagcctt ttcgccgtca
    22081 gtttttcctg gaaggcgtcg atgtcgaccg tgcccacctt ggcgatcttc gcgaaatcga
    22141 aaatgctgat ctgcggggcg ccgtggtatt cggtgatcat caccgtcatg tccaactccg
    22201 tccccttttc gaggaacatc ttcctcgttt cttcgggcat cttttccatc accgaatcga
    22261 agaccgtcat gccgatgctg ccggtctcgt cgctgagcat gaagacgtag aagaccgatc
    22321 ccttcttggt cttcttttcc tcgtaggagg aaacgaacac ccttatctcg acgatctcgc
    22381 cgatggactc ggttttgatg tcggcgatgt tgtatttctt gggctccagc tttttcagga
    22441 tacggacccg ctcggggacg gggaggaaca gcccgatgcc gaatttctgg gtctcgctga
    22501 ccgagccggc cacctcgacg aggtcaccca gccccggcag gttgtcttta tgtatttttt
    22561 tgaactggga aagcttgttg aacgccatca cgccgatctt cccggtgccg tcctcgatgg
    22621 tcatcttcaa ggtgttccgc gggacgtctt ccacataaga ggcgactttg ccgacgacct
    22681 tgatgtaggc gccgttcatc cgttcgtcga tgtcctcgac gtgtatttgt ttgggttgtt
    22741 tgacgtgggc ggcataccac aggagaagga cgcccaggac ggaccccgcg agggaggcga
    22801 tcttcacgat tttgacggcg atgcgttttt ccacccgcgc gccgcagcgc ggacaagaca
    22861 gaagggatcc ggcgaaataa ccgcaggtcg ggcagagccc ctcctcgtcc acaatgaccg
    22921 cctgcgtctc cgggggcgcg ctattttcct gtcgattgtc tgtcatcttg ggtcaatggg
    22981 cgttcgtttc ggctctttct tcgtgaatga acagattcct tgctgtggga aggggaacta
    23041 ttatggcgca ttctcgcggg aatggcaatc gcggcgaaca agggcggggg ccttctttcc
    23101 ggcggcgccg accggccgca cataccggtc atcccgcaga cgctgcgcat cattttcgac
    23161 caggcacggg gccagcgccg cacaggtgca gcgatcgacc gggcagggcc ggggggcgtc
    23221 caaaagtcgg aaggtcgggt ctttcgcatt tccgaacggc tcgttggcgt tggggcatcg
    23281 ccggagggac ccgtccgcgg ccaacacgaa ccctttctgg ccggcccggc acatcttatc
    23341 tttggtgcgg atgtcccata tctgatatct cacggccttg tcgtcgccgc ccattcggat
    23401 cagctccttc tcctcgggcg aatgggcggc cgggtattgt ttcccctgat actttccgtg
    23461 aaacggccgg atccaacata gaacgccgtg tctccggaag ccgtcgtaat aggtccggat
    23521 atcgttcaaa agcggcggat aggccaccat ggaggtcatg accccgatac cccgcgcctt
    23581 caacgacaat attttctgaa ggagggtgtc atgggagatg aagtaggggt ggaaactcgc
    23641 gtgaagacgg acccggccgg ggcggagggc cggaagccat tcgtccggct cccacgacaa
    23701 attcgtggtg atggacaggg tatgtttttc agaaacgctc cgcagtattt caaaaaaatc
    23761 tttcgtcagg aacggctcga ccttgcccgc gacgccgatg ttcatcggcc cgtaaagcga
    23821 atgggcgcgg tcccaaaaac cgatccactc cgccgccgag agttccgccg gttcaggaac
    23881 ccactcgccg acatacccgc agtaagggca cccgaaattg caccgatccg cgaggttcca
    23941 aaggcaaaag atttgttctt cgaattttgt ggcgttcatg gggagcacgc gaaaaccggc
    24001 gggcgcggcc aacggctccg ccgtcgattt ttcattatcc taccatgttt aaacggcggt
    24061 ttcgttatgt ttttaccgtc ccccggtcat cgggggacgc ggacggcggg tcagtgcggt
    24121 ttcggcgaga accttcggcg gaaccaggcg gcggcgtcgt cgaacaggga gtaggccacc
    24181 ggcgtgatga gcagggtgat gaggagacac agagtctggc cgccgatgat gaccaccgcc
    24241 atggtggcgc ggttggccga gccgggtccc ttgcccagag ccaccgggag catggcggcc
    24301 accaggacga ccgtggtcat gaggatgggc cgcaaccgcg tcttgttggc ctccaggatg
    24361 gcgtggtccc gttccatccc ctgggcgcgc agggtgttgg tgtaatccac ctgcaggatg
    24421 gcgttcttct tgacgatgcc gaagagcatg aacaccccca tgatggagaa aatggtgagc
    24481 ggctgtcccg aaagccacag ggagaacagg gcgaacggaa tggacagggg cagggacagc
    24541 atgatcgtga cggggtggag gaaactttcg aactgcgacg ccaagatcat gtacatgaag
    24601 atgaacgaca ggacgaacgc catcacgaac ccggccagca tgcggttcat ctccttggtc
    24661 tttccgacgg tctccgtttt atactccggc ggcattttca gttcccgggc gatccggttg
    24721 ctggcgctca aggcgtcccc gagggcggcc ccttccaggt tggcgtaaat ggtcacctgc
    24781 cgctgccggt tcaggcggtc gatctgcgac ggcccctcgg cttccgtcag ggtcgccacg
    24841 ttgtccaacc gcaccaggcc caccttggag gacggaatga agagcccccc gatggattcc
    24901 gcgtccttcc gggactcctt gtccacgcgc acccggacct gatagagttc gtccccttcc
    24961 ttgtacttgg tgatgtcttc ctccccgctc accatggccc gcagcgccat ggcgatgtcc
    25021 tgcacctgca cgcccaagtc ctgggcgcgg tcccggtgga tctgcacctg cagctccggt
    25081 ttcttgagga tgaggctggt gtcgaagtcc accagccccg gcactttctt gagttcctgg
    25141 atgaagcggt cggaatagtc cttgagtttg gaaatgtcgg gtcccgtcag cgtgtattcg
    25201 aaatccgcct ccttgccgcc gccgtggaac ccgcccgccg ggacgacgct gatggtgagg
    25261 ccggtgtatt tggacagcgc gcggcgcgtc agcgccatga tctcggcctg cgtgtggcgc
    25321 cgctccttgt aatccaccag ccgcacgtag acttttccct ggttgacgcc ggcgccctcc
    25381 tgttccccga tggacgacag aaggctcctg acggccggga gccgacggat gtccgcctcg
    25441 atctgtttca ggatatcgtc ggtcgtggag agcgaggtcc cttccggggc gcggatctcc
    25501 acgttgaagt ccccggtgtc gtccgacgga aggaagtcct tcccgatgat cgcgagaaag
    25561 acgccggacg acaggacgat gcccgccgac acccccacga tccacatccg gtggcccaag
    25621 gaccaaacca cgagccgccc gtagtggtcg cgcaggacgt cgttgaaccg gtccacccat
    25681 atctgcagag cgttgcgcgg tccgtgggaa agccggagga acttggcgca caacatcggc
    25741 gtgagggtga acgcgatgaa caggctcacg gcgatggcga aggccacggt gagtccgtaa
    25801 cttttcagga accggccgac gatgccggac atgtaggcca gcggcaaaaa gatgacgatg
    25861 agggacatgg tcgtcgccat gacggccagg ccgatctccg cggtggcttt ggaggcggcc
    25921 tcctccggcc gtatccgctt gtgctccata tagcggtgga tgttctccag caccacgatg
    25981 gagtcgtcga tcacgatccc cacggccagc gccagggcca acagggtcat catgttgagg
    26041 gtgaaccccg ccgcctgaat caggatgaac gtggcgatga tggaggtggg gatggacatg
    26101 gcggcgataa aggtgctgcg ccaatcgccc atgaacagca gaaccgccaa cccggccagg
    26161 agcgccccca acacgatgtg ttcctccacg gtgcgcaccg aatcgatgat gaaggaggac
    26221 tggtcgttga tgacctcggc cgtgacgccg ggcggcaggg cggccttgag gtcgccgagg
    26281 cgttccttga cggtttggat cacctgcacg gtgttggtgc ccgactgttt ttggacgatg
    26341 agggagatgg tggtgcggcc gtccaaccgg gccatgctgc gcgcctcctg ctcggtatcc
    26401 aacacccggg ccacgtcccg gatgcggacg ggaacgccgt tggcgttggc gacgatgact
    26461 ttttcaaaat ccttggggga ccccacgcgg ccgagcgtgc ggagaatcag ttcccgcgag
    26521 tcctggtcca cccgcccgcc ggggatctcc acgttctgct gcttcaacgc cgccttcacg
    26581 tggtaaatgg aaaggttgaa ggccttcagc gccgcgggat tgaccaccac gtggatctcc
    26641 cgttcgcggc cgcccacgat ggtgatcttc cccacccccg cgacggtctc cagcggctcc
    26701 ttcaccttgt tcttggtgat ctccgtgagc tgctgcatgg ggtagcgtcc gctcaccgcg
    26761 atggaaacca ccggcagggc gtccggatcg aacttctgca ccacgggcgg gtccgtcccc
    26821 tcgggcaggt ccttcgacac gcggttgacg gcgtcccgga cttcctgggc ggcgacgtcg
    26881 gggtcctttt ccaggacgaa ctggatcagc acctggctca gcccctcata actgacggac
    26941 ttgaggagtt cgatcccgga aatgctgttg atggcctctt cgatcggctt gctgatggag
    27001 gtttcgatct cctcggggct ggcgcccgcg agggtggtgc tgacaatggc gaacgggaaa
    27061 tccacgttgg ggaactgatc cagccccaac cgagtgtagg acaccgcccc cagcaccacg
    27121 agggcggaga ccatcatggt cgtgaagacg ggccttttta tgcagacgtc aacgagtttc
    27181 acaaaaggtc cccttgaccc gacgcagaaa cgacaccgcg gggatgcaaa tcatctccgt
    27241 cccccgtccc gtccgttttt tttaaagagg gcgaatgttc cactttcgtt tttcactcga
    27301 ccggggacga agtcccttcg atcaattcca ccggactgcc gtccttcagc gcgaagagac
    27361 cgaaggtgat gacctggtcg ccttcgttca atcccttgcg cacctccacg cggttctccg
    27421 tgcgaaggcc cacctccacc tcgcgcttga ccgccttgcc gttctcaaag acaaagaccg
    27481 cggggccgct gccgttcacc agcgcctccg ccggcacgga cagcacgccg gcccgatgct
    27541 ccgtcacgac ggagacctgc acgaacatgc cggacttgag gagtcctccg gaattttcca
    27601 accgcgcctc gaggggaagg gaccgagtca ccgggtccac cgcggggctc acgcgcgaca
    27661 cgcgcgcgat gaacgaccgg tccggcaggg actccaggcc gatccggacg gcctgtccga
    27721 cacggacccg gccggcgtat cgctcgggca cttccgcgcg cgcgatcagt tcgctggtgt
    27781 ccagcaccag ggccacgggg gtgtccgttt tcacgtccgc cccctcatcc aaatacgtgc
    27841 gccccaccac cccgtccagc gtggagggca ccggggcggg cttgaactcc acccccacct
    27901 cgtcccgttc caccaaggcc acggtctgtc cttttttcac cgcctcgcct tcctttaata
    27961 cgtttttcag gagtttcccc ggcacgcggg aatagagcgt ggcctcgttc ttggctttga
    28021 gcgagccgac cacgctcaaa gcgtcctcca ggttttggcg ggtgagcttc tccacccgca
    28081 ccgggaacgc ctccagtttc ggccctccgt cctcccgggg cccgcatccc gccaggagca
    28141 aaacgccgaa cgtgaaaaga aaatagtttc tcggaaatga cttcatggct gaacctcctt
    28201 caaattcgtc cccaccgccc gttcgagggc ggcgatgttg acccaatagt cgtgggtggc
    28261 ctcggcgaac tgcagccgcg cgcggttgaa ggcgaacgtg gcgtcggtca gctccagctg
    28321 gccggagtcc ccttctctat aacgcacttc ggtcgcttcc agtgcccgac gggcctgctc
    28381 caccgccgtc tcctgcgcct tgacccgggc cgccgcttcc cggacggcca gccatttctc
    28441 ctgcacctcc cgccgaaccg cgcgttcggt cttttcctgc accagcctcg ctttttcgtg
    28501 atcgatcttc gcctgtcgga ccttctgcca actctgtcct ccctgaaaga gattgaattt
    28561 cagcgtcaat ccgccgttca cgctctcggc gtcttccccg ggatcgaggt gtttgtcggc
    28621 cgactgcgcc gtccactggt aattggcaaa ggcgtccagc gtgggtttat aggaagcggc
    28681 cgcgatggag acgtagcttc gggccaccgc ggtgttgctc cgaaccgcca gcacctccgg
    28741 tcgatgcgac atggcccatt tttccaaaac agcgttttcc ggcggggcct gaccgacggg
    28801 atcgaacgcc ccttccgcct ccaagggccg gtcgatgtcc aaagagagga cgtccttcag
    28861 gttcaaccgg gccgtctcca gcatgttgtc ggcgctgatg agaagcgttt tggcgttggc
    28921 cacctccacc tcctgccggc ggaggacgag atcgctgtcc aggccctggt tgtagcgcgc
    28981 gcggatggtg tcgtaatgac gctgggcgga atccaagttt tctttctgga tggcgacgat
    29041 gtcccgcgcc aggagcacgg cgtaaaacaa ccgcaccacg gcaaggtcca cctcgtcgcg
    29101 cacgccgcgc agcgccgctt cctgggcccg gcgcgcctgg tgagccgccg cgatggcctt
    29161 ggacaccttg ccgcccgtat acaacggctg ggtggccccc acggtggcgg aggcggcgtt
    29221 cttttcgccg atcttgatgg cgttgccgcc gaaaaaaatg accgactgtt taaagctccg
    29281 cgtgtaggtg cccgtcaagt cgatggacgg gaacgcgctg ccgatgacct catggtacaa
    29341 gctgtcgaac ctctccagat cgtattcggc caaggccacg tcgatgttct gtttcgcggc
    29401 cagttcccgc gcctggagaa gcgtgagccg gtagggctct tccgaccccg ccgcggccgg
    29461 ccaagcgagg gtcgcgctca gcaaaaacag caggccgcgc ctcatgggcg cttctcccgc
    29521 cccacgccgc ggaggaaaaa gtcccacgcc gtctccagcg tgagcggggc gctgacaccg
    29581 ggctccaaca acgaggcctc caggaagaac atccggaaca gggccatcag catcaaggcg
    29641 ccgaggttcg ggtcgtgttc gcgcaactcg ccggactgaa cggcggcgcg tattttctcc
    29701 gccaggaacc gcacgtactt ggcgaaatgc agacgaagcg ccccgccggt gcgcttgccg
    29761 tagatctcgg gctttgtcag cgatatctgc gaaaaaaatt ccttgtgttc cctgaaaaaa
    29821 accagctgct cctccgtgat ccgccgaagc tgctggatcg gcgacagggt gcccaggtcg
    29881 gggagggacg ccatgcgggc gttgatgtcg gaggcgatgg attggaagag ggcgtcgaac
    29941 aaatcgtcct tgtcctggaa atagaggtag agcgtcccct tggaaacgtt ggcgtaacgg
    30001 gccacgtcgt ccaggaggat gttgtggaat cccttttcgc ccaaagcggc ctcggcggcc
    30061 aggaggatct gttttttccg tatttccggg ttatcgattc tttggattgt catttttgtc
    30121 tgctgacttt cgagtcagta acattttacc cccgccaccc ccttttgtca agggctatcg
    30181 tcgaacaaat gccgtaaatg cgttattttt gaatttaatg ccgttatttt aaataaatta
    30241 ctgtctttcc agtcattttt gaatttacgc ccccccgctc cccggcgccc ccgaaccccg
    30301 ccggcggaaa cgagcgcgcg gagaaaacga tttccaggcg gacagctgaa atgctaaact
    30361 tttttccggt tcaccggtct ttttccggtc cgaccctcac acatgaaaat aatttccgtc
    30421 tcatccgagt ttcccaccca ggcagaaacc aaactcatca ccatcagccg ggtgcgggac
    30481 gcccagtgtc cgctgaggta ctacaaaaat ttcctggaaa aaccccggca ggaaccgctc
    30541 ttcatcccca tcgagctggg gatgggcggc ttcttccacg actatctgga gaaacacctc
    30601 aaactggtga tgaacctgca cacggagatc gacaaacatc atcggatcga cgtgaacgac
    30661 atgctccgct cgttccgcat gtcctttgtc tgggaaggga aactgcggcc accgtacaag
    30721 atcgtcctgg aaaaactgga cgtggaagac tacgtgtcga aactgcgggg cgtgggccag
    30781 aacttcaacg atttcctgat ggaggaactg ccggggaaac gcatcctggg cgtggaggag
    30841 ccgatccagc tccggacgga aaaagccacc ctttacggga aatacgacct catcaccgaa
    30901 gacagcgacg gctccatcac cctctgggac tggaaaacca acatcctgcc cggctcgcaa
    30961 agccatgaag agttccggaa ccagaagatc cagctgggga tgtacgccat ctggctgaaa
    31021 tacaaatacg acctggacaa ggtgcgggcg acggctgttt ttttcggaga caagacggaa
    31081 caggtctcgg agatgttttc cgacgaggtg gaacgggacg tcctgacctt tctgacggcc
    31141 tggcgggaca aactgaacaa actggacagt tatcccccgc ttcccaacaa atcctgcccg
    31201 tggtgcgaat ggtatcctgt ctgtcccgcc tttgccacgg gccggaaacc cgccccggcg
    31261 gtccgcgagg gcgccgcctc tccttccccg tcggcggctc cgctcccctt cgcgcctcgc
    31321 cggccggagc acgaagaaca cctcctggcg ctggtgctgg gcgtcgtcct ggccctggcg
    31381 ctggcggtgg cgatgttttt ccccttcctg caaaaacttt tttaagctat ttccgcccca
    31441 atccccggtc ttcttccagg ctcgaattaa aggaatagga tttcaccgtt ccgtccttat
    31501 ggaaccggac atagaggtcc cgcgtgtcct gggaaccgaa caatcgcacc ttgtagtgca
    31561 tgtaggtcca ggtctcgtcg ccgtcgtcga tgccggtccg ggccggttca ccgaaatccc
    31621 tcaacagggc ctgctgggtc gtttttccca cttcgatggc cggcacgagg ccgacaggga
    31681 acggtttccc cacggtgacg catccggcca agaacgctgc caaaccgatc agataaacga
    31741 tacgcatgag ttcctcctgg aatgtccgtc ccgcccgttt ttcacacggc cggaactgat
    31801 aaaatcgggg tcatgggccc ccgcgccgaa gccctcccgc tcctcgtgct ccactacgac
    31861 caagccgccc aaacggcgcg ctgtctcgcc tccatcctgc aggccgggta tgcgccggaa
    31921 caaatctatt gtctcgataa cggctcccgg acagaccccg cggaacccct gcgccggcgc
    31981 tatccccgcg ttcagttccg ccgcatcgaa aaaaaccggg gcttctcggg cggagtcaac
    32041 gaggcgctgc gatgggcgtt cgacgaaggt ttttcatccg ctttttttct cagcaacgac
    32101 acggtgctcc accgggatgc gccggaaaaa tgccgggaaa cggccgcgac cgcgggagcc
    32161 gggatggtcg cgccccgcgt cctttcgctg aaatccccgg cgacgacgga atccatcggc
    32221 ggggggttcg atccggccgc cgccgccctg cgtcatcacc gagctcccgg cctgcccccc
    32281 ctcctcggcg aaaacgacta tgtccccggc gcggcattct ggctgtcgcg cgacaccttc
    32341 ctgtccttga acggcatgga cgagtcctac catacttatt gggaagacgt ggacttctcg
    32401 ttccgggcgc ggcgacgggg aatcctcctg gcccggtgtt acgacgcccg ggtcggccac
    32461 gcgataggaa agacctgcca caaaaacccg ctctacaccg cttactatta ccagaggaac
    32521 cgtatccgtt tctgcgagaa attcctgacg ccgaaacaac gcgagacggc cacgacggcc
    32581 atccgcgacg acttggaaaa gttggcccgc cgctggggac aaaaaaacga cgcggagcgg
    32641 ctgaactatc ttccggcgct gcggcgcgaa ttggccgggg aacgaagccc cgcccccttt
    32701 ttgtcgtaac ccagggaggg gtccaaggcc accgctgttt taaaactttc ttccgccgct
    32761 cggccccgtc ccaaccgctc aaggaccatc cccctgttgg cctgggcgaa cgcgtccccg
    32821 ggatccaatc ggaccgcggc atcaaaatgt tttaaggccc cctcaaaatc tcccaaccgc
    32881 cccagaacga tccccgcctc aacccaggac ttctctctcc ggggatccaa cgccaacgcc
    32941 cgccgcaggt ggaccaacga ttccgacaag gccccccgct gcaaaagcag gcttcccagc
    33001 gccgccgggg ccgctgagaa atccggccgg agcgccaccg ccgcgcgcag atgtttttcg
    33061 gcctccggag cgcggccggc ccgccgaagc atgacggcga aattgtaatg cgtttccgcc
    33121 gaatccgagc ggctattcag cgccgccacg tagtgcgcct cggccttttc cgtttctccg
    33181 atctcggcaa aggcgttggc cgcgttcagg tgcgcctcgg gaaggccggg gtccagccgc
    33241 agggcggtct catagtcgcg ccaggcctcg ctccgacggt tgagcgccca gaggaaattg
    33301 gcccgattgg cgtaccccgc cgcgaaacgc ggccgaagcc ggaggagggc gtcgtattgt
    33361 tccagcgcct tttcatactc ccctttttta aaatacgcca ggcccaggtt gtagcgggct
    33421 tccggatagc cgggacgcag gatcagcgcc cgccgaaaag agcggatggc cgccggaatc
    33481 cgattttccg ccacaaggag gttccccagg ttattgtgcg cgagccagga ctgcgggttc
    33541 ttttggagag tgtcccgcca aagggtctcc gcgtcgcgga attttccggt ttccgtccaa
    33601 gtgcgccccg ccaacacgag cagcagcgcc gccgccacgg ggaccgcgat tttccccttt
    33661 tttatcctcg aaccgacaac gccggcgcca aggcccaaaa gaccgaggct ggccagatat
    33721 tgaaaatgat cggccacgaa cgaaaaacgc attggataaa aatcaaaaaa tcccagcgcc
    33781 ggagcgaggg tgccggcgaa gaagaggacc gccgccaggg gcccgcgccc gattttttgt
    33841 cgttgaatcc agagcgccgc gaaaacggcg attaccgccg ccgggaacag gacgtgcccc
    33901 cacgcggtca tctcgatcgt ccatcgggga tagatgaacg tcaagccgga gggccaggcc
    33961 agttttccgg cataaaacca gagcgcccgg ccggcgatca ggattctctc caggaatgcc
    34021 aaatcccatt cccctcccat ggcgccgacg aagcgccttt ccatcgtcga agagagaaag
    34081 ccggcaagga ctcccagcgc gagaaagggc gcgagcggag acacctcccg ccatcgcagg
    34141 cggcctcttt gccaccagat cgtcaacgcc atcgccgccg gaaaagaagc cgtcacgctc
    34201 ttgctcatga gcgcacacaa gaacagaccg agggccgccg cgtaacagct cccccttcca
    34261 cgaccgggcg gggccccctg tcgcgacttt tcaaatttca aatagcacaa gaacgcgccc
    34321 aggtaaaaaa agccggataa aacgttcttg agttccgtga cccaggccac cgattccgcg
    34381 tgcaccgggt gaacggcgaa aagagccgcg gcccaccacg cccacggcac ggacagcgcc
    34441 gccagaacgt tccatgccag cacagcattc gcgccgtgca gggccacgtt caccacatga
    34501 tacccggccg gagagaatcc ccacagcttg tgttgcaccc aaaacgcggt gtagagcagc
    34561 ggataggcct ggggcacggc gccgtattcc gtccatatcc gccgcaaccc ctcgcccgtc
    34621 cgcaaagagg cgttttgcat cacgaaattg tcgtcgtccc acacaaaccc cgcccgatag
    34681 gcggggccgt aggccgccaa ggtcagcaga agaagcgcgg cggccgggac cgtccgtctc
    34741 catcgccgaa acggccacga tccgtccacc ggccgccggc cgatcatttc gttcatgccg
    34801 gacgatctta gggagctttt ttgggcgggg actgtttcag gaggggggtg gggtcggcca
    34861 atccccaatg ttccacgatt tgaccgtcga tcaatcgaac gacgtgggcg gattcgatct
    34921 cgagacgacg gccggtggcg ggacggccca tccacgggcc ccgctgcacg cccgtcaggc
    34981 gggcgcggac catcacctta tcgccgtcgg ccaggacatc gtccaggacg accgtggcct
    35041 ccggaaaagc gttgaaaagc ccggccacgt ttgccgtcac gccgccgtaa tccggcgcga
    35101 cgccgcgggg cggatgatgg tccacataac ggggagagac gtactgcggg acgacgccga
    35161 tgttgtggcg gttgaaaagt tcctccacca gccgtcggac gcgctccttg ttccgttcct
    35221 cgacggcggc ccggatccgg agccgcgatt cggccgaaac ggcgagctcc agagacgcca
    35281 cggccccgtc gcgcaccacg gcctttttcg acagcaaaac cttttcgatc ccgagggcgc
    35341 ggtaatggtc gcttgccatt tccgccaccc aggaaacgac ctccccgtcc gccttgaaat
    35401 cgcgcgacgt gcttcgatat ccgcgaagat gggtcgacag gaaatcccgc atcccctcct
    35461 tccccacata gcgctcgccg ccctccgcca ttgtcagaac ggcctccgcc ccgaagaatc
    35521 ccgacacccg ctctggatct tgcgtgttcc aagcctcctc gaacgcctgc gccgtcgcga
    35581 tgggtttcat cggacgcgcc tgcgccggcg acgaccacaa aaacaggact gccgctgctg
    35641 caatgccgat ccatccgggg gacggtcttt tcattttcga tttctccctt cagccgatgt
    35701 cttttgtgcc actttacaaa aatacgaagc ggccgccaaa gccaatcgcc aaaagttgat
    35761 tgcccggccc gactcttaat atcctaaaat caaccccgct ggcaatgttt cggcccgagg
    35821 tttaccgaat gccgaccgct cgtcccatcc acacccccca tcccgacgtc cctccttcgg
    35881 aaaaaatttc tccggcggag ggcctgctct ccggctcact cgcccgcggc cttgaaatct
    35941 ccctcttcct cctgatgctc tccttcttcc tcgcctcggt gttttatcgt tttgattccc
    36001 cggggatgat ccgggaccaa ctgctggatg aggtgcgcgg ccatcacatg gcgcagcaac
    36061 tgcgtgccgg acagcccttg ttggagacgc tcgacatccc ctttcccctg tatcatggtt
    36121 cactcatctc ctatctcatg atgccttttt tctatttcgt gggacccagc tggacgctcg
    36181 tccgcgcgtg ggccgtggga gccgggctct tggccctgat tctttcccac ctcttcttga
    36241 ggcgcgcctt ttcccccgcc gtcgccctcc tcaccctttt tctcctggtc gtccatccgc
    36301 ccttcatcat ggggataaag atggtgaact acaccgcccc caccctgctg gttttctccc
    36361 tcggggcttt gttgctgttt cagaacgggt ggcgcacacg aaaccctctc tattttttcc
    36421 tcgggacggc ctgcctttcg ggggggatcg gcatgcgatt gtggttcgcc tggtttatcg
    36481 gggcgctcgc cggatccgcc ctcgtttttt ccggaccgat ccgccgtcac ttcgacttcc
    36541 cccggtcccg gaacctcctc ctctctctgg gactgggcgg gctggccttc ctcccctggc
    36601 tttcattgac tcttcgcacc gaattaagcg ggcgctggga ctctttctcc ctcctttcct
    36661 gggtctatct ccggccgtta tcgattctct cccgcgcgtc ccatgccgcc tccgcggacg
    36721 ccctctggga ccccctcttc cgggccgccg ccgaggtgtt ccacagcttc aacgcgaatt
    36781 tttttcaagg ccgcttttac ttttcctata ttttttcgtc cgacgcgccg gtcacgaaca
    36841 cgtattatcc ctttcttttc tggacctgcc tcgccctcct cctgtttctt ctgctccaac
    36901 ccaaaaattt cccgcttcgg ccccaagcgg gcaggctggt cgtttttttc atcctcctct
    36961 atctccaatt gttcactctc tataaagaac cgctccacca tacgtacctg ttttatcctt
    37021 tcccgcaact catcgtggtg gtggccttgt cgtctctttt cgccctggtg cgccgccgaa
    37081 cgcctctcct ttgtctcctg cttccgttcc tcggcctctt cctgatccaa gaattccgtt
    37141 ccttgcggaa ttattatgtc cacatccggg atttcggcgg acgggggccg gccacggatt
    37201 cggtctatgc cctggcgacg tggatggaga agaacctttc ccccaaagaa acggtcctgg
    37261 tttccgagga gtttcttggg gaacatttgt atttcttgtg cccggacttc aagacaagcc
    37321 ccccgttgtt tccattccgg gttatggacg acgcgcatat aaaactgagc atcacttcca
    37381 tgtggcaaaa taccatcaag acgtacggag cttttatctt catcaacggt gtgtacgtca
    37441 aacgttcgac atattttttg ccgatcgatc tttttgatca cgtgccctgg tccgccaagg
    37501 acgcccccga attgctggcg agtttccggg accgggacgg ccatcccact tttgacgtgt
    37561 atcgctttaa ggagaccgcc catgaccgaa aacccctccg acgaccccct ccacgtcgtc
    37621 tatgacgacc cgcggctgat cgccgtcaac aaggcggccg gccagctcgt cgtccccgga
    37681 cggggagaga cgggcgaacc gctcaacgcc cggctggaga agctcatcgg ccggaaaatc
    37741 tatgtcgtcc accggctgga cagagaagcc
//
LOCUS       DAOTFD010000007        35134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_807_length_35134_cov_4.340118, whole genome shotgun sequence.
ACCESSION   DAOTFD010000007 DAOTFD010000000
VERSION     DAOTFD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 35134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35134
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_807_length_35134_cov_4.340118"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..499)
                     /locus_tag="P5079_01045"
     CDS             complement(209..499)
                     /locus_tag="P5079_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HRY28602.1"
                     /translation="MKTREVVISGKKAVGFEIPLPGAPLVLVRAEKGFVMCGYLNPAA
                     AEKLGVAAAVVRGVKTVDELLAKPVAEISPEAQKLGVTPGMTGRQAVERFLA"
     gene            656..1414
                     /locus_tag="P5079_01050"
     CDS             656..1414
                     /locus_tag="P5079_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY28603.1"
                     /translation="MKKQKLSIILVSYNTLPYVKRCLENVRQRVRRPHEIVCVDNASR
                     DGIAAYMAARPRLKFRRLPENRGFAAAMNEGMRMADGDLFLWLNADALLTEGAVERMI
                     AVLGRRSDVAGVGPCTDIPPLPQGCVPAPRRTADVERVAMAWRLRHAGQVEEVRGLTG
                     FCFLTTRKAVARVGRLDERFYPAGYEDYDFCARALRKGFRFLLAREVFVHHDGRCSYR
                     SRSHWKRIWTENRRRFIAKWGRRFAVPYLAELCS"
     gene            1524..2957
                     /locus_tag="P5079_01055"
     CDS             1524..2957
                     /locus_tag="P5079_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRY28604.1"
                     /translation="MSESVLVANAAKCSESHKAQWESLRSQNPGIDKILAAAGKPQGL
                     ALVFSHDDPDGITSGLIFKRLLEKKGWRTVWKMPAGFVLTTAQFDAALKAHPDAKAVF
                     VLDKGTLAPYADFGKRMPVYLVDHHPSPKAPTDCVLFNPCLEKYASCSGSFLAHGLAT
                     LAGTRDDYDDFLALIGLKGDWAIEPTKGILSDMVKPFFAEYAMKFRNLLQLVTERPTM
                     FDAEQRELTCPLSRFAEFVHAVGGGGFQYFYNDRDASLRDVDHPACIGAALEKMAGKV
                     AELKKITTLDDFIKLLPEPEAGSLKKIWKFFLEDWEKAFRLLDSSTKILGLADTSLYV
                     FVGGKVPLLPMIGSIKLFELKQAAKDKFAQIIMVSSVSADYTHVSVRGTGDQVHSGKF
                     CGQLAGALQTKFPKQKDVISGGGHPRAAECTVRTGEVPFIKVLHEVVLQLNEMSELDN
                     RSRAAAEGSHQARAKELGLDYLSLAKA"
     gene            2962..3369
                     /locus_tag="P5079_01060"
     CDS             2962..3369
                     /locus_tag="P5079_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28605.1"
                     /translation="MTFKETEFPATLKYLKAAMAADPDPLLIKEVLAQIIKLYEAVPL
                     YPGIANMCAAQAVKAVEPKDVAVGQKIYFQKGNVSYLGTVAEKTADGLKLKAVKAAEE
                     DVSVSLKDMSKVKAINEKILEELWPSLVFEKVK"
     gene            3366..4622
                     /locus_tag="P5079_01065"
     CDS             3366..4622
                     /locus_tag="P5079_01065"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HRY28606.1"
                     /translation="MKEQKIGKEEFFLLVGPAQFAVTSGAVEVIAARIGKGEKLNVPL
                     GKRVPIRALEDTVLQVECEPDKLTKMEGSSIPAEWDALAKRVVGERRDGRLYSILVLG
                     EVDTGKTFFSTYIANRLLHHLKRTAILDCDTGQSDIGPPGTFGMLVMKKPVVFLTEEP
                     ATYLYMLGAHSPGLHFLPALTGLAAMLEKARADADALIIDTTGWVQGDGGRAIKKSKL
                     DIVNPDMVVLMQRGTELEHLVKHLPKERVVRLPVSKKASSTSQMERKSLRELVSVRYF
                     KGAQKMEIPFSRVFTDRCYFLTGTPLTLDGALHAERLSAWEGSLAVTAKPLTPDVSKG
                     WPKEIGRVTNFVAGDEKGLMVGLLDDKQNCLAIARLDEIDFAKKCFRLRTPFSGSPEQ
                     VRGIQFGSLKLAEDGREAGFIEPGSI"
     gene            4699..5226
                     /locus_tag="P5079_01070"
     CDS             4699..5226
                     /locus_tag="P5079_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRY28607.1"
                     /translation="MKMRCDIHINNAFRKLLLVQEREETLEHMALRLSAFILFWDFDP
                     KMAVPMDRPPLAGQEFRPDIIGLTPGGEIALWGECGNVTLHKLNKLTRRYPSSKIVVL
                     KSSEQAGQKLREDLRKDRTVREKQIEIWCWPAAAFASWMRAMDETVHAVGEVSGRSLN
                     LTINEVPLAVDLRSA"
     gene            complement(5247..6878)
                     /locus_tag="P5079_01075"
     CDS             complement(5247..6878)
                     /locus_tag="P5079_01075"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28608.1"
                     /translation="MLKNRLSVMRYGDVLEFLGFGGLGAVFLFVLYYGFVRLLWEVHS
                     VEMIGSLLIVKLMAMAFLTTFLMIVFSSTLASFTTLFFAQDLPFLVQSPLPFRSVFLF
                     KAVETAAFSSWMVLLAMWPFLAAYGFVYGLGKGFWVFLAAMAVPFVLIACTLGIGLSL
                     ALMCVFPSRRVREVMLLLGVFVGGSLYVLFRWLQPEKLVRADSFEVLIQYIALLEAPL
                     APYLPSWWMTSAVAAYTADRGAGLLADAGLLAGAAVLLGSILVFFAEKAYYFGWTSAQ
                     ESGRRQGTSALGREWRWVPRFFPSGFRGLVGKDALLFVRDANQWSQLLLLLALVAVYL
                     ISIDKLPLDNAYLKGLISFLNIGMVGFVLSSVALRFVFPAVSLEGKCWWLVRTAPFGL
                     GTVLMEKVLLGFFPLCVLGVALVWASNRLLGVDVFVTRLSVTTIGVMSFALCAMGVGL
                     GARFPRFGVENVPQIETSPGGLLFMVSSLFYVGATISLEAVLMRMHYYSVVRANSAWD
                     APGTALVLAALFLLNAAVVLGPLLAGKRYLERADV"
     gene            complement(6924..7673)
                     /locus_tag="P5079_01080"
     CDS             complement(6924..7673)
                     /locus_tag="P5079_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY28609.1"
                     /translation="MIKTQGLSKRFRDIVAVDRLDLEIPPGEIFGFLGPNGAGKTTTV
                     KLLTGLLRPSAGRAWVGGFDVQTHPREAKRILALVPDQPYVYPQLTGKEFLRLVGDFY
                     EVDLSHQKKKIPEFLEMFELGEWGDQLVETYSHGMRQKLVLAGVLLHQPRVLFLDEPM
                     VGLDPKSARLVKDVFRNLAGKGVSIFMCTHVLEIAEKLCHRIGIVQEGRLGLVGSTEE
                     LRRKADSSGSLEDVFLRLTGGDEYHELLKYL"
     gene            complement(7678..8277)
                     /locus_tag="P5079_01085"
     CDS             complement(7678..8277)
                     /locus_tag="P5079_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28610.1"
                     /translation="MKIVLPQELRRKFFHLFTFLYVLVYAAAGKNVSLWIFGTFLLVA
                     AAGEAVRLRSPAANAALMKLFRGIHRAEEQHRPSGILWTALGVFLVVLFVPYPDVVIT
                     ALCYLAVGDTAAALVGRTWGHLRIGGKSMEGSLACFLACWLTGSLTLASPFGSVELIV
                     GALVATLAEILPLPLGDNLWVPVLSGAALSLLRLTVSPF"
     gene            complement(8274..9050)
                     /locus_tag="P5079_01090"
     CDS             complement(8274..9050)
                     /locus_tag="P5079_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28611.1"
                     /translation="MKKLFSKTAGFSSRSWTSPLLAAAFLPLLAAALWTTWRLLCELA
                     GKRLPLTPVLAGAGGYVLLHLLFQKPMRLYVFGHELTHALAAWLSGFRVKSMKVSAKG
                     GEVSLSDSNLLVALSPYCVPFYTLTALGLFEVSRRFVGFAVPPFWYAFTIGLTFSFHA
                     ALTGHALLQNQPDLRYAGTFFSLVLILFSNVLVFVLLLKILFPANVPLKPLAAHFVSA
                     TLIILDRLAQFAVWAGVKGWTFSKTLFGKVGPLALKFKNP"
     gene            complement(9065..10504)
                     /gene="hydA"
                     /locus_tag="P5079_01095"
     CDS             complement(9065..10504)
                     /gene="hydA"
                     /locus_tag="P5079_01095"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRY28612.1"
                     /translation="MSFDRIVKGGKVFTADDSREADVAIKDGKIAAIGKNLPADGAEV
                     LDASGLWVIPGGVDVHVHFQLRVAGTVSADDFENGTKSAACGGVTTVVDFSTQGPRDD
                     TLLAGVEARLKEAEGKACVDFGLHAGISAYKRLKDPAADLRRLLDFGVPSVKMFMIYE
                     QEGWQSDDADIFSALRLARGLGATVCVHAESERVMNLLIAEHRAQKERLGAWAHALSR
                     PNFIEEEAVQRALKWAEATGGHLYVVHLSTGGGADLFKQAQARGVNAHVETCPQYLLL
                     NDEVFKDKKTGHWYATCPQIKKKADNERLWQGLADGTIAVVSTDTCPFTTKQKDAWNG
                     DFTKIPYGLPGVETLLPALYTFGVKAGRFGMNHFVSLISTNPAKLMGLYPQKGAIAVG
                     SDADLAMFDPDAVRTIDSKELVSRCDWSPYQGLTMGGFPVHTLSRGEPVVRNGKFVGP
                     KGHGRFLKRRPFGWKDLPEPRLVPPSGGR"
     gene            complement(10539..11234)
                     /locus_tag="P5079_01100"
     CDS             complement(10539..11234)
                     /locus_tag="P5079_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28613.1"
                     /translation="MKEWLQKRWVQNTLLVAGTAAVAYLMVFIDVQARAREAYRQGET
                     SLRWAQNPDEKKQFYEEKFLREKKKLDRRREKAEISQEDYDQDLEVLKFDRDQVVGES
                     SLKYAYQWFKDTYELFSPPESKWVRRARLMAPAAKERWREELRAKNIPFEEYMLDLEI
                     GEEPGTLMVYSTRGRKESERAAELLRSKGIEPKVYDAPAHGRLPQEGLKILVPRDKFW
                     EAHEILKAAGFGR"
     gene            complement(11278..11679)
                     /locus_tag="P5079_01105"
     CDS             complement(11278..11679)
                     /locus_tag="P5079_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28614.1"
                     /translation="MDTNGSTTGKKIVVVIVEDDHYILDFMKFTLEQQGYEVIPAADG
                     QEGLDMIQSHLPILVVLDLMLPKIDGFTILKRMNEHAETAKIPVVIVSAYTASQSTRR
                     MVQSQKNVREVFTKPIRSKEFVAKLKTIIGA"
     gene            complement(11765..12643)
                     /locus_tag="P5079_01110"
     CDS             complement(11765..12643)
                     /locus_tag="P5079_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY28615.1"
                     /translation="MRICSFLPSGTEMVCALGLTDHLVGVSPDCDYPPEVKTKPVVLK
                     NALDLSELSSRGLDRHVSDRLHKGEKLLRVDGRRLNELRPDVVIARAATDGEIPPVSK
                     TLYLAPRTLEDIFENFRTVGNTTGTLDKAKDIVYDLRLRAMAAQCNAYRDKQIPRVAV
                     LHWMDPPYLAGLWVPEMIEIAGGKPGPASAGKDSLPMTWDQLAEFNPDVVVCAPCGHH
                     LAGAMKEAALLAKCPFPGKIGAFRDNRLYVVDGDAYINRPGPRVVDGIELLAHLLHPE
                     FCTWSGPAAAFQKIAL"
     gene            12842..13528
                     /locus_tag="P5079_01115"
     CDS             12842..13528
                     /locus_tag="P5079_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY28616.1"
                     /translation="MGKKLFLFDLDGTLVTTGGAGLRALDRAFESLLGLRNAMEGVSP
                     AGKTDPLIVREVFVEKLGRVPSADDTRAVCESYLRYLPEEVKNGSGYRVMAGVRETLR
                     ALAAREEMVLGLGTGNLERGARIKLAPAGLNDYFPFGGFGSDAEARPEVLRAGVRRAE
                     ARSGERFSPRDVFVIGDTLLDVEAGKAIGATTVAVAAGHAGLEDLRAGNPDILLESLA
                     AERFWEGLPR"
     gene            13671..15617
                     /locus_tag="P5079_01120"
     CDS             13671..15617
                     /locus_tag="P5079_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRY28617.1"
                     /translation="MIAFGRGICGRLSESGEKEWLETNGRGGYAMGTVAGCNTRRYHG
                     LLTVPLRPPVQRFQVLNRLEESVFINNTRYPLSCQAYPGVVHPHGYHYLERFRLDPFP
                     VWTYTVSNVRLEKTFFLRYGEDTAVVLYRHLAGPKIAIETRPLVTCRDHHHIVREDER
                     FKDRFEISPHLVKLKCTSDHTLFMGAEEGTFNQDSFWYLNQEYEWEKMRGLEHREDAF
                     SPGLFRFELDLGRTVALVFSTQERKSSEALDWSRQERLLRKQILDKSAVRGPLADRLV
                     LAADQFIVDRDKGSSVIAGYPWFEDWSRDAMISLPGLCLATGRTDEAWEMLELYAAHL
                     KEGLLPVRFPDADAPVDYNAVDAPLWFILAVQKYLKATNDTERVKRLLPAMRQIADSY
                     QRGTQFGIRMDQDGLIAASTDGQALTWMDAKVENTPVTPRSGKAVEIQALWYNALQFL
                     TELDFKLNEPSRGYDKLAAIARASFNEKFWNESAGYLYDRINGRDRAGAVRPNALFAI
                     SLPYEILEESRFRPVVDTAWRELYTTYGLRTLAPGDPNYKGKFEGPPEDRDAAYHQGS
                     VWAWLMGSFLTAYVKAYGPSETTKAQVDEFLRPLLDHLTEGGVGSFSEVFEGDAPHAP
                     RGCPAQAWSVAEILRVLWEEGVVV"
     gene            15749..16699
                     /locus_tag="P5079_01125"
     CDS             15749..16699
                     /locus_tag="P5079_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28618.1"
                     /translation="MKTAYRTGCQVCHQPRDNRLTPYAREYVRLNGGFAALDALDVMD
                     PDKDGFPSKEELGFHSNPGDARSTPAHIGDWLSNLTPSEPPREILVDLYGRIPPYEVF
                     ERPLKRHQAQSLERALGQKLRDEELFPVIFLFNKKGEAGAPPAPDRGAYAFCWGKKMT
                     SLFAVVAPEGTLRAVRFVRLYGDQRFDNEQYLSQFPGRTAETLQGVSSPRGAEAQNDD
                     ILSALRRCLEIMDVAAPVSVTPAALKDVSPAEDDGWYYRPRPSEPLPEYPEPPLKPLP
                     IAPVPPAPAEEPNGVSPENGTDQEPATEGANPNTEDTNGR"
     gene            16689..17456
                     /locus_tag="P5079_01130"
     CDS             16689..17456
                     /locus_tag="P5079_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRY28619.1"
                     /translation="MVGDIVGVGAAFLAGVLTFLSPCVLPLIPVFLTFVTGVSFEELS
                     ASASPDGGTRSQRRTTLLHVTAFILGFSFVFVLLGASATFLGRVLFSHQEWFLRGGGV
                     LIVLFGFLMMGLLPVGFLQKDFRVHLRQKPAGLAGSFLVGAVFGFGWTPCVGPILGSI
                     LLLASSSDSVRQGMFLLAVYSLGLALPFLAAALLFNGFLRFFRKIRWVMVWLERAAGA
                     FMVLVGVLVAFGQFSRLTGWMLQAFEPWVDWLIRLGI"
     gene            17471..18463
                     /locus_tag="P5079_01135"
     CDS             17471..18463
                     /locus_tag="P5079_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY28620.1"
                     /translation="MAPKDVYDVTIIGGGPVGLYAAYYAGFRALRVKIIDSLAELGGQ
                     VTALYPEKMIRDVAGFPGILGKDLVRNLAEQAFQYKPVIGLSEQVQELTVTGDKEIRL
                     LTDKGEHFTRVVLITAGIGMFTPRKLPLPEADRFEGKGLFYWVKHLEEFRGKRILVVG
                     GGDSAVDWVLGLLSVAKEITLVHRRDVFRAHEDSVRKMMDSSARIKTFHEVKSILGRD
                     AITGAVIFNNKTGQEETVSVDTVVACLGFLATLGPLETWGLELDGRSVKVNFRMETNR
                     PGIYAAGDMASYPGKVKLIATGFGEAATAVNNAAAYINPKSTVFPGHSTEKKQS"
     gene            18460..19530
                     /locus_tag="P5079_01140"
     CDS             18460..19530
                     /locus_tag="P5079_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRY28621.1"
                     /translation="MNARHRFFAAAVLWGSLGGFAAAQEEITPPVDEQPESAKIFLQA
                     WPMADLRREPSPPMDNAALKSPYDADPFQETQLLFCEEVRLREESGAWARVEAVEQPE
                     FSHAQAWGGYPGWVLKKSLVPKPAGFSANAVVAVKYARLHEEPSKKSGYVEIPLGSRV
                     SAQPSAPWALIRLPNGGEGWTSLKSLRFFENLPKDDGALRKAIVKTAELFVGEPYYWG
                     GRCSHRTKVLDRPTGVDCSSLVNLAYRVNGVDVPRDAHEQYLKSLPLERRQMKPADLI
                     FLAKADDPNRVVHVMLSLGDDLVLEAVQEFDAVRVTTVKNKLGKSLSEISPLEPAGRY
                     VVYLGRLLPDPAAAAASPDTPR"
     gene            19533..20651
                     /locus_tag="P5079_01145"
     CDS             19533..20651
                     /locus_tag="P5079_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRY28622.1"
                     /translation="MQEKFRLSVLRVVPVRWPLRRPFVTSLGAKSYSDNVRVMARLSG
                     GATGYGEASSSLAMPFQTAANMSAALRRLFHSLRGSDARDIEKIVSAAWRLEGKYPTA
                     VAAFETALWDALSRALGMPFSVLWGGASTTIETLMSVSAVDPEEVSARVRAGRRRGFR
                     LFKLKLNGHEPPERDRARLRAAHRACPRGRFLADANQSYSPENLSAFLAAARRDGLSV
                     EVLEEPFKKRSWAALASVRATVRTPLLLDESVQTPADARRAARGRLARGVNVKLAKSG
                     LLRGHHILREFWGEDERFRRSAASPRPLFMIGCMAESKLGLAASVHWACGLGVFDYAD
                     LDSDLLLKPTPCRGGYVRRGPTLSLPRPLPPGLGVEPA"
     gene            20648..21796
                     /locus_tag="P5079_01150"
     CDS             20648..21796
                     /locus_tag="P5079_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRY28623.1"
                     /translation="MIRSPALLLAAFVGVVALVLHARRRGAFQAVFRFLPVPFWCYLL
                     PMLGTTAGLFPSDAPLYGALSRYLLPFCLALLLVSADLSAVARLGARALGVMAFSVAG
                     IVLGAVFGFVLFQDRLPEDAWKSVGALSGSWIGGSANMLALKEALSVPDAIFAPVIVT
                     DTLLAYAWMAFLIAGAGFQKRFDAWNAAAVPTFGLAGVSPAPTARPGFRAAPLLAAIG
                     AAGFSIFLGDRLPALGTAVTHATWTVLIVTSLSLALSFVPAFSTAGGLTEKWGATILY
                     ILLASMGARARFTDIFQSPRFLALGVVWLAVHGLALLAAARFLRAPLALLATASQACV
                     GGPVSAPIVAAVYHPSLAALGLLLAVLGNVLGSYVGFLTAHLCRWAAG"
     gene            21819..23762
                     /locus_tag="P5079_01155"
     CDS             21819..23762
                     /locus_tag="P5079_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY28624.1"
                     /translation="MSLKKIVGFCFCLAAVAGVPSALWAQNMDMLPDMSVDDLLPPPT
                     VYEPVPTMPEAWGAPAPLRLVHPAEGARLPFLKSSFVFGSADPQGKLSLNGQPVPIHP
                     EGGFLAMIPYSTGTFSIRAELVLPNSTVIAVRTVSVAGPTLASPRSPLTVEYVRPDLD
                     TTVRPGDVVSVICKGSPGMEAVFQVQGVHPRFPMMEDASAGVKGVYRGMFVVQPGDEL
                     NDNWIRVTLTDKERGRKIVHESPGRLSRLNDRVPWVVAVTTDNAVVRAVPALASGDKG
                     GYLLFPPPGTRLQVLGRQGAELKVKLGSSRTGWISEGDVRRLPDGTPPPITLAGTVSV
                     ASDGARSTLVRLILKQKVPFLVEASPDKKQLDISFFGAASNTDWIHYASTGAVERIQW
                     FQDDPETYRLRVHAPAGDWWGYDTRYEGTTFVLELRSPAPPAAHQPPLAGLRIAVDPG
                     HSADRGSIGPTGLLEKDANWAIAQCLKKKLEAAGAEVVFIREGSQNVPLYDRPKLAWQ
                     ARADLLVSVHNNALPEGGDPLEKNGYSVYYFQPHSFELAREIHRAYRDILGASSRPAA
                     LRDDGLHYGNLALARPPQMPAVLTESAYIIVPAEEALLKKEPFQCDCAEAMLRGIVRF
                     VSSARGGPSPLPKNSRDKRSKSN"
     gene            complement(23769..25256)
                     /locus_tag="P5079_01160"
     CDS             complement(23769..25256)
                     /locus_tag="P5079_01160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28625.1"
                     /translation="MRRLDKHTLGGVLIVALAAFFSLHGLDREILWQDESETALVAKT
                     VLARGLPFGTDGKNVFSQNRGADYTKDFLWRWHPWLQFYLCAGSFAVFGVGTFSARLP
                     FALLGVATVALLMLFVRRRWEDPRRALWAGAALTFSVPFLLLAREARYYSAAAFFTLL
                     GLYAYDRLTEKNKSGFLLLFCASFFLFHSHYVLCGALLAALLLHASVFSRDRFPPLLA
                     ACLAVSVVNLPAVWLFWGTDRGVGLFNAGRALDFFRYYASETLEHIFPLLLLLFPLLA
                     VVMTGRKPSFSPRDRSSAALLSMVAGVTFVFLAVLSPANYFRYLAALIPLACAGEGWI
                     LRFLQDVWGIVPAAALLMGLAVYGGLPPFLYEITHRYKGPMDGIVDYLRKNGRDSDVV
                     AITYEDLPLKFYTRMRVVGGLTGEDLSPALKADWIILRKHTVSDKDEQVKNYLLEKVA
                     WPDYEKITIDFPDIPYENRETPDEHLFHTMTREDRVVIHRRKRTT"
     gene            complement(25271..25414)
                     /locus_tag="P5079_01165"
     CDS             complement(25271..25414)
                     /locus_tag="P5079_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28626.1"
                     /translation="MCLAFAINAFLGRPKESLLGVGVAGLGIPLYFFSERRTRRGQAS
                     SPC"
     gene            complement(25544..26635)
                     /locus_tag="P5079_01170"
     CDS             complement(25544..26635)
                     /locus_tag="P5079_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRY28627.1"
                     /translation="MGAMNNETQPALRRGLNVWDAGAIVVGCIIGAGIFRLSDSVAGH
                     SSSGFMFLLAWGLAGLLSLCGALTYAELASLFPKTGGDYVFLTNAYGRLWGFVFGWTK
                     LFVQRVGTISILAFVFAEHTCRATELAGGPGAVKSLATGGIVLLTATNIVGLRAGKTV
                     QNVLTALKVLALLGIIAVGLFAGKGSAENFQPFWPEWSWNIWHATGLAMIFALWTYGG
                     WENAAYVAEEVREPERNLPKALVFGLLGTTVLYLVVNAVYLYYLPLPELRETDLVAAG
                     AMDKIWVGRGGQVVAAMVMASTLGALNGYILTGSRILFALARDHALFGKLALVSPRTR
                     TPVMSLLTTAALATLLVWTGTLDQFVTYT"
     gene            26756..27208
                     /locus_tag="P5079_01175"
     CDS             26756..27208
                     /locus_tag="P5079_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRY28628.1"
                     /translation="MNDHLKAFRNYLKEQGLKNTSQREEILAFLGRSSRHLSPEEIFR
                     ELRRRDPRLGRATVFRTLRLLGDCGLATRVTFPDGKQKFETRLGGHHHDHMLCVHCGR
                     AIEFVSPAVEEAQKRLARRYGFKVLWHRHEIFGRCRRCETAGPSPRRS"
     gene            27283..28101
                     /locus_tag="P5079_01180"
     CDS             27283..28101
                     /locus_tag="P5079_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRY28629.1"
                     /translation="MGARRKRAALFLAATAFAASAGAEAPAKIQDNSFLLEEAYNQEP
                     GVVQHIQAFQYLTKDKTWTASFTEEWPWKGQKNQLSVTLPVEHFPAPGNDTGLGDVGL
                     NYRYQALLDDRVAASPRVTLLLPTGDEEDGFGAGALGYQVNMPFSVDLSDRWVTHWNF
                     GATYTPDSRAAGSVRADTLGFNAGFSVIHVLSDRLNLMMETAATSEETPLPAGGTERN
                     DTFFLNPGVRGALNFESGLQVVPGLAFPIGVGPSDGEHGVFLYLSFEHPLRSGG"
     gene            28142..30130
                     /gene="feoB"
                     /locus_tag="P5079_01185"
     CDS             28142..30130
                     /gene="feoB"
                     /locus_tag="P5079_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRY28630.1"
                     /translation="MEKAAAVLDDKKVVAMKRLALVGNPNVGKSVVFNRLTGQYVTVS
                     NYPGTTVDVSRGGGRLGGENYEIVDTPGVNSLIPNSEDERVTRDLLLRENPDVLVQVA
                     DAKNLHRALLLTLELAEFRRPMLLVLNMMDEAMSRGLVIDPARLSRRLGIPVLRTVAT
                     LGEGLADVKAAVARAAVPSAAVPYPPEIHRSLEALDGAWRTDFSPKRAVSLALLSGDG
                     SALTWAHPGLRPGETEESLWRRADAQRAAFVRPPRLVVFEARDGWVKETLGEAVYRRA
                     GPSGNWASRFGNWCMRPWPGYLIALAALYAVYQFVGVFGAGVAVDFLEGTVFGRYIVP
                     AAQRLADLLLPSTFLRDMVVGPYGIVTMALTYAFALIFPIVTTFFLAFGILEDSGYLP
                     RLSVMMDRLFRLMGLNGRAVLPMVLGLGCDSMATVTTRVLDTAKERLIVTVLLSLSVP
                     CSAQLAVILGMTAGMSPRVFLLWLGVVAGITFVVGWGSARFLPGARSTFVMEIPPLRR
                     PMLRNLLLKIKARLEWYLKEVVPLFVLGTLVLFFLDKFGGLKRLEVLGAPVVQKFLGL
                     PAAATQTFLIGFLRRDYGAAGLYHLQMEGLMNDRQVTVSLVLITLFMPCIAQWLMVVK
                     ERGWKMAAVVTAVVTVIALAAAGGLNALLSVAPRLISS"
     gene            30145..30825
                     /locus_tag="P5079_01190"
     CDS             30145..30825
                     /locus_tag="P5079_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRY28631.1"
                     /translation="MTEDKKFPIPTAEKEDIEEGLNVVWNMFEKKVRRRAEIQARLTE
                     AVGEKALDLLMREGLLRESGDELVFSPEGERLAKDIIRRHRLAERLLSDVLSMARAEL
                     DSNACRLEHIISPEISESICTLLGHPRQCPHGSPIPSGDCCRREEENIRPIVVSLDNM
                     KLGEEGRIAYLLLHDHPELHKLLSLGVIPGAEVRLHQTFPSFVLELGDSQLALEEAVA
                     RNIYVRKK"
     gene            30881..31174
                     /locus_tag="P5079_01195"
     CDS             30881..31174
                     /locus_tag="P5079_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28632.1"
                     /translation="MDTLPVKHQKVSEVLEPEIVGLEPAVPFWKRAVRRVTAGLALGF
                     VGLGMTVLGGLFTLTIVGAGIGLPLVFLGILVMVFAVFLFFADRSTVSIRFRR"
     gene            31286..32062
                     /locus_tag="P5079_01200"
     CDS             31286..32062
                     /locus_tag="P5079_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRY28633.1"
                     /translation="MKKITLFLSVLLSAGLSFAEFEGVLHIKMSAGPTSGNGTILVSK
                     SGVRNELNMQTPQKPVRMTSLLTAKNPATLYVINDEKKTYSEMDLDKTRRAAEKAERA
                     SYKVKRLPDETLLGYRCAHVFIAGSGGESELWTSREIMDYDTFARMNAGKDRGPADGA
                     YGKALKEAGADGFPLKSIHRAGKEGVTVVEVVRVEKKPISPAMFEVPAGYQKTGLLGA
                     GPAATSMPPDAMKRMEEQMKNMPAEQREMIEKMMKQGGQP"
     gene            complement(32100..33710)
                     /locus_tag="P5079_01205"
     CDS             complement(32100..33710)
                     /locus_tag="P5079_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28634.1"
                     /translation="MENPSRFLPRFVKKTGLVLLGAGLALLFLETGLRLTGGALRAKQ
                     NLSLRKALNSGGDVRLLCLGESTTAGGYPKHLEEILNRRQTKTRFTVIDAAYPGTTTS
                     VILTQLEKNLEKYKPQLVVTMMGINDAEDDGFEHRPDPALSPASWRVTHLTKLVASSL
                     RERFRKTSSPGDLRAAAGTGDAVQRLSPAMKTLLATIRHQRERQQFDDAVDSCRRALA
                     IEPANVLFLLELAHCFSDRGMCAETEKICRDILRIDPASESASIEMAACYNSEGNDPK
                     TVKLAEAVLKINPRSVPAFLLLATVHYRAGRHADMERACRNALAAQPRNVRAQVLLSK
                     ALKLQGRLDESPETLQEFISLKPGALRARLELAGLYCLRGNHEKAEKVCLQAVGLLSE
                     GRTHPERQARAAVHGALGIVYSQMGRPIQAAEHFEMAEMDQNFSEKTRRNYLRLWETL
                     ARRQIVYVCASYPLRSTAPLKEIFGPNAGVLFVDNEKPFREVLKKEPFSKYFTDLFAG
                     DFGHLTKNGDILLAENVADVVLKEFSKN"
     gene            33833..>35134
                     /locus_tag="P5079_01210"
     CDS             33833..>35134
                     /locus_tag="P5079_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRY28635.1"
                     /translation="MRHFFRFEERATNFRTEVVAGITTFLTMAYIIFVNPGILSAAGI
                     SFPAAVTATALGSCLICVTMGLVTNRPLALAPGMGVNAVVAFSVIGINQANVPWQVGM
                     SVIFLEGLVILGLVLLGLRGAVMDAIPLNLKRAIGIGIGLFITLIGLNQGGLIRPAPI
                     TLVALGDFSQKYVWVTLIGLGAILIFMALRVKGALLWGILSATGAALCLRVTRPPTQI
                     VGPLDFQTFFAPFQRVGGRMALGDVFTGLLLLTVFSIMMTDFFDTMGTVVAVGEQGGF
                     VDKRGKVSGMKKILTVDSLAALVGGLFGSSSITTYVESAAGVAVGGRTGLTAIVTGLL
                     FGAAAFFAPVIQMVGGGYRIPNADQYRLFVNTGFQTPADAIPPAGAGDFFVYPITAGA
                     LIIVGFLLMKTISQIDWNEFEEAFPAFLTIVGIPLTYNISYG"
BASE COUNT         6139 a        10886 c        11704 g         6405 t
ORIGIN      
        1 gagtttgtcc cctggggcgc cgaacggaac gaacaccacc gcgccgtgat gccgcgccag
       61 cgcgtcgccg ccgagcgcga gggtctcggg ggaaagcgtt tctatctttt cgggaagggg
      121 cgtccgccct tccggaggca caaagggacc gtgtttttgg gcggggttca ttgaagggaa
      181 gggaggccga tccggggaat ccgcttcgct aagcgaggaa ccgttcgacg gcctgtcggc
      241 cggtcatgcc gggggtgacg cccagttttt gggcttcggg ggagatctcg gcgacgggtt
      301 tggccaggag ttcgtccacg gtttttacgc cgcgcaccac cgcggcggcc acgcccagct
      361 tttcggcggc ggccgggttg aggtagccgc acatcacgaa ccctttttcc gcgcgcacca
      421 gcaccagcgg cgcgcccggc agcgggatct cgaatcccac ggcctttttc ccgctgatga
      481 cgacctcgcg tgttttcatg gcggcctctt tcgttcggaa actttcgggc aggtgagaga
      541 gaatcatatc tttcgtcggg ggaattgtca aaccgagcgg ttttttgccc gccggggcgg
      601 ataaaatata gaatgagcgg cggcaagcca ttttcagcga aacggggatg tccggttgaa
      661 aaaacaaaaa ctcagcatca tcctggtctc ctacaacacc ctgccgtacg taaagcgctg
      721 cctcgagaac gtgcggcaac gggtgaggcg gccccacgag atcgtctgcg tggacaacgc
      781 ctcccgggac ggcatcgcgg cgtatatggc cgcccggccc cgcctcaagt tccgtcgtct
      841 gccggagaac cggggattcg cggccgccat gaacgagggg atgcggatgg ccgacggcga
      901 cctttttttg tggctgaacg ccgacgcgct gctgaccgaa ggggcggtgg aacggatgat
      961 cgccgtcctc ggccggcgtt cggatgtggc cggcgtgggt ccctgcacgg acatcccgcc
     1021 gctgccgcag ggctgcgtgc ccgctccccg aaggaccgcg gacgtcgaac gggtggcgat
     1081 ggcgtggaga ctgagacacg ccggacaggt ggaggaggtc cgcggcctca ccgggttttg
     1141 ttttttgacc acgcggaaag cggtggcgcg ggtggggcgg ttggacgaga gattttatcc
     1201 ggcgggatac gaggactacg atttctgcgc gcgggcgctg aggaaagggt tccggttcct
     1261 gctggcgcgg gaggtgttcg tccatcacga cggccggtgc agttaccggt cccggtcgca
     1321 ctggaaaagg atatggacgg aaaaccggcg gcggttcatt gcgaagtggg ggcggcggtt
     1381 cgccgtgccg tatttggcgg agttgtgttc ctgagaccgt tccaaaatat ttgacggttt
     1441 ggcccttgcg ctttcgcttg ccaagtttta tgattatccc cttcgcaaca attcgtccag
     1501 cctactaagg aaggtccgca accatgtctg aatcggtttt ggtcgccaac gccgcaaagt
     1561 gttccgagag ccataaagcg cagtgggagt cattgcgttc gcagaacccg gggatcgaca
     1621 agatcctcgc ggcggcgggc aagccgcagg gcctcgccct ggtcttcagc cacgacgacc
     1681 cggacgggat caccagcgga ttgatcttca aacggttgct ggagaaaaag ggctggcgca
     1741 ccgtgtggaa gatgcccgcg ggtttcgtgc tcacgaccgc ccagttcgac gccgccttga
     1801 aggcccatcc ggacgccaag gcggtttttg ttttggacaa gggcacgctc gcgccctacg
     1861 ccgatttcgg gaaacgcatg ccggtctacc tggtggacca ccatccgtcc ccgaaggcgc
     1921 ccaccgactg cgtcctcttc aacccctgcc ttgagaaata cgcctcctgt tccggctcgt
     1981 tcctggcgca cggcctggcg accttggccg gcacccgcga cgactacgac gatttcctgg
     2041 cgctgatcgg cctgaaaggc gattgggcca tcgaaccgac caaggggatc ctgtcggaca
     2101 tggtgaaacc gttcttcgcg gaatacgcca tgaaattccg gaacctcctg cagctggtga
     2161 ccgagcggcc caccatgttc gacgccgaac agcgcgagtt gacctgcccg ctctcgaggt
     2221 tcgccgaatt cgtgcacgcc gtgggcggcg gcgggttcca atatttctac aacgaccgcg
     2281 acgcctccct gcgcgacgtg gaccaccccg cctgcatcgg ggcggccctg gagaaaatgg
     2341 ccggcaaggt ggcggagctg aaaaagatca ccaccctgga cgacttcatc aagctgttgc
     2401 ccgaaccgga agcggggtcg ctgaaaaaga tttggaagtt cttcctggag gactgggaga
     2461 aggcgttccg cctgctcgat tcctccacga agatcctcgg cctggccgac acctccctgt
     2521 acgtcttcgt gggcgggaaa gtgccgctcc tgccgatgat cggctccatc aagctgttcg
     2581 agctgaaaca ggcggccaag gacaagttcg cgcagatcat catggtcagt tcggtgtccg
     2641 ccgactacac gcacgtgtcc gtgcggggca cgggcgacca ggtccattcc ggcaagttct
     2701 gcggccagct ggccggcgcg ctgcagacca agttccccaa acagaaggac gtcatcagcg
     2761 gcggcggaca tccccgcgcg gccgagtgca ccgtccgcac cggcgaggtg ccgttcatca
     2821 aggtgttgca cgaggtggtg ctgcagctca acgagatgtc cgaactggac aaccgttccc
     2881 gcgccgctgc ggaaggaagc caccaggccc gcgccaagga actgggcctg gactacctga
     2941 gcctggccaa ggcctaacga tatgaccttc aaagagaccg aattccccgc gacgctcaaa
     3001 tacctgaaag ccgccatggc ggccgacccg gacccgctcc ttataaagga agtgctggcc
     3061 cagatcatca agctctacga ggcggtgcct ctctatcccg gcatcgccaa catgtgcgcc
     3121 gcccaggcgg tgaaggcggt ggagcccaaa gacgtggccg tggggcagaa gatttatttc
     3181 cagaagggga acgtgtccta cctgggcacc gtggcggaga agaccgccga cggcctcaaa
     3241 ctcaaggcgg tgaaggccgc cgaggaggac gtgagcgtct ccctgaagga catgagcaaa
     3301 gtgaaggcga tcaacgagaa gatattggaa gagctgtggc cgtcgctcgt tttcgagaag
     3361 gtaaaatgaa agaacaaaag ataggcaaag aggaattttt tctgttggtg gggccggcgc
     3421 agttcgccgt gacctccggc gcggtggagg tgatcgcggc gcggatcggc aaaggcgaga
     3481 agctcaacgt gcctctcggc aaacgcgttc ccatccgggc gctggaagac accgttttgc
     3541 aggtggaatg cgaaccggac aaactcacca agatggaagg cagctccatc cccgccgagt
     3601 gggacgcgct ggccaagcgc gtcgtggggg agcgccgcga cggccggctc tattccatcc
     3661 tggtcttggg cgaggtggac accggcaaga cgtttttcag cacctacatc gccaaccggc
     3721 tcctgcacca cctgaagagg accgccatcc tggactgcga cacgggccag tcggacatcg
     3781 gcccgccggg aaccttcggc atgctggtga tgaaaaaacc cgtggtgttc ctcaccgagg
     3841 aaccggccac ctacctttat atgctgggcg cccattcgcc ggggctgcat ttcctgccgg
     3901 cgttgaccgg cctggcggcc atgctggaga aggcccgcgc cgacgccgac gcgctcatca
     3961 tcgacaccac cggatgggtg cagggcgacg gcggccgcgc catcaagaag tccaagctgg
     4021 acatcgtcaa ccccgacatg gtggtcttga tgcagcgggg cacggaactg gagcacctgg
     4081 tcaaacatct gcccaaggaa cgggtggtgc ggctgccggt ttccaagaag gcgtcgtcca
     4141 cgtcgcagat ggaacggaaa tccctgcgcg aactggtgag cgtgcgttat ttcaagggcg
     4201 cccagaagat ggaaatcccg ttttcccggg tcttcaccga ccgctgttat ttcctcaccg
     4261 ggaccccgtt gacgctggac ggggccctgc acgcggaacg tctttccgcg tgggaggggt
     4321 ccctggcggt gacggccaaa ccgctcacgc cggacgtgag caagggctgg ccgaaggaga
     4381 tcggccgggt gaccaacttc gtggccgggg acgagaaggg cctgatggtg ggccttttgg
     4441 acgacaaaca gaattgtctg gccatcgccc ggctcgatga gatcgatttc gccaagaagt
     4501 gtttccgtct ccgcacgccg ttctccggtt cgccggaaca ggtgcgcggc atccagttcg
     4561 ggtccctcaa actggccgaa gacggacggg aagccggttt catcgaaccg ggaagcattt
     4621 agcctttagg cgctcgccct ccgaccgcaa cgccgtcctc cgctccccgt cttttcgttt
     4681 tgtccccggg ataaaaccat gaaaatgcgg tgcgacatcc acatcaacaa cgcctttcga
     4741 aaactcctgc tggtgcagga gcgcgaagag acgctggaac acatggcgtt gcggctctcg
     4801 gcgttcatcc tgttctggga tttcgacccg aagatggccg tccccatgga ccggcccccc
     4861 ttggcggggc aggagttccg gccggacatc atcgggctga ccccgggcgg ggagatcgcg
     4921 ctgtgggggg aatgcggcaa cgtgaccctg cacaaactca acaagctcac gcggcgctac
     4981 ccctccagca agatcgtggt gctcaagtcg tcggaacagg cgggacagaa gctgcgggag
     5041 gatttgcgca aggaccggac ggtgcgggag aagcagatcg agatctggtg ctggcccgcc
     5101 gcggcgttcg cttcctggat gcgcgccatg gacgagacgg tccacgcggt gggcgaggtt
     5161 tccggccggt cgctgaacct gaccatcaac gaagtgcctc tcgcggtgga cctccggtcc
     5221 gcttaggacc gccgttaaaa ctttttttaa acgtccgccc tttccaaata gcgtttgccg
     5281 gcaaggagcg gccccagcac caccgcggcg ttgaggagga agagcgcggc cagcaccaac
     5341 gccgtccccg gcgcgtccca ggccgagttc gcccgcacca cgctgtagta gtgcatccgc
     5401 atcagcaccg cctccagaga gatcgtcgcc cccacataga acagactgct gaccatgaag
     5461 aggagccccc ccggggacgt ctcgatctgg gggacgttct ccacgccgaa ccgcggaaaa
     5521 cgggccccta atcccacccc catggcgcag agggcgaaag acatcacgcc gatggtcgtc
     5581 accgacaggc gcgtgacgaa cacgtccacg ccgagcagcc ggttggaagc ccacaccagc
     5641 gccaccccca acacgcagag cgggaaaaag ccgaggagca ctttttccat caggacggtc
     5701 cccagcccga acggcgccgt ccgcaccagc caccagcatt tgccttccag gctgacggcg
     5761 gggaacacga accgcagcgc caccgacgac agaacgaatc ccaccatgcc gatgttgagg
     5821 aacgagatca atcctttgag gtaggcgttg tcgagaggga gtttgtcgat gctgatgaga
     5881 tacaccgcca ccagcgccag gaggagcagg agctgcgacc actggttggc gtcgcgcacg
     5941 aagagcaggg cgtcttttcc aaccagcccg cggaacccgc tgggaaagaa gcgcggcacc
     6001 cagcgccatt cgcggccgag cgcgcttgtg ccctgccgac ggccggattc ctgcgccgag
     6061 gtccagccga agtaataggc cttctcggcg aagaacacca ggatcgaccc cagcagcacc
     6121 gccgccccgg ccagcagccc cgcgtcggcc agcaggccgg cgcctcggtc cgcggtgtag
     6181 gccgccaccg ccgaggtcat ccaccaggac gggagatagg gggccagcgg ggcctccagc
     6241 agggcgatgt attggatgag gacctcgaac gagtcggcgc ggacgagttt ttccggctgg
     6301 agccagcgga acagcacgta gagggagccg cccacgaaca ctccgagcag gagcatcacc
     6361 tcccgcaccc ggcgggaggg aaaaacgcac atcagcgcca ggctcagtcc gatgccgagc
     6421 gtgcaggcga tgaggacgaa cggcaccgcc atcgccgcca ggaacaccca gaaccccttg
     6481 cccagtccgt acacgaaccc gtacgccgcc aggaacggcc acatcgccaa aagcaccatc
     6541 caggaggaga acgccgccgt ctccacggcc ttgaagagga acacggaccg gaacggcagg
     6601 gggctttgca ccaggaaggg caggtcctgc gcgaagaaga gcgtggtgaa ggaggccagc
     6661 gtgctggaga agacgatcat caggaaagtg gtgaggaaag ccatggccat gagtttcacg
     6721 atgagcaggc tcccgatcat ctccaccgaa tgcacctccc agaggaggcg gacgaacccg
     6781 tagtagagga cgaagaggaa gacggcgccc aggccgccga accccaggaa ctccagcacg
     6841 tcgccgtaac gcatgacgct caggcggttc ttcaacatga ggagacggtt cttgaggagg
     6901 aggaacatgg ggacgcgggg cggctacagg tatttcagga gttcgtggta ttcgtcgccg
     6961 ccggtgaggc ggaggaagac gtcctcgagg gatccggagg aatcggcttt ccgtcggagc
     7021 tcctcggtgg agccgacgag cccgagccgg ccctcctgca cgatgccgat gcggtggcag
     7081 agtttctcgg cgatttcaag cacgtgcgtg cacatgaaga tgctgacgcc tttgccggcg
     7141 aggttccgga agacgtcttt caccagccgg gcgctcttcg ggtcgaggcc caccatcggc
     7201 tcgtcgagga agaggacccg cggctggtgc aacaggacgc cggccagcac cagcttctgc
     7261 cgcatgccgt gcgagtaggt ctccacgagc tggtcgcccc actcgccgag ctcgaacatc
     7321 tccagaaatt cggggatctt ctttttctgg tgggaaaggt ccacctcgta gaaatcgccg
     7381 acgagacgga ggaattcttt cccggtgagc tggggataca cgtagggctg gtcgggcacc
     7441 agggccagga tgcgtttcgc ctcgcgcggg tgggtttgga cgtcgaagcc cccgacccag
     7501 gcccggccgg cgctgggccg cagaaggccg gtgaggagtt tgacggtggt ggtcttcccc
     7561 gcgccgttgg ggccgaggaa cccgaatatc tccccgggag ggatctccaa gtccaggcga
     7621 tccaccgcca cgatgtcacg gaagcgtttc gacaggccct gggttttgat catgttctca
     7681 gaacggggaa acggtcagcc gcagcaggct caacgccgcg ccggagagga ccggcaccca
     7741 gaggttatcg cccaacggca gggggaggat ctccgccagg gtggcgacga gggcgcccac
     7801 gatgagctcg accgatccga aaggggaggc gagcgtgagg gaaccggtga gccagcacgc
     7861 caggaaacac gccagactgc cttccatgct tttgcccccg atgcgcaggt ggccccacgt
     7921 ccggcccacg agggcggcgg cggtgtcccc gacggccagg tagcacaagg cggtgatgac
     7981 cacatcgggg tacggcacga acaggaccac gaggaaaacc ccgagagccg tccagaggat
     8041 gcccgaggga cggtgctgct cctcggcgcg atggatcccc cggaagagct tcatcagggc
     8101 ggcgttggcc gccggagaac ggaggcggac cgcctcgccc gccgcggcca cgaggaggaa
     8161 cgtcccgaat atccacaggg agacgttctt ccccgccgcg gcatagacca gcacgtagag
     8221 gaaggtgaac aggtggaaaa atttccgtcg aagttcctgg gggaggacga tcttcatgga
     8281 tttttgaatt tcagggccag cggcccgacc ttgccgaaga gggttttgga gaacgtccat
     8341 cctttgacgc cggcccagac ggcgaattgg gcgaggcggt ccaggatgat cagcgtggcg
     8401 gagacgaagt gggcggccag gggtttgagc ggcacgttcg ccgggaaaag gatcttcagc
     8461 aacaggacga acacgagcac gttggagaac aggatgagga ccagcgagaa gaacgtcccc
     8521 gcgtagcgga gatccggctg attttgaagg agggcgtgtc cggtgagggc ggcgtgaaaa
     8581 gagaaggtga gcccgatggt gaaggcgtac cagaacggcg ggacggcgaa cccgacgaat
     8641 ctccgtgaga cctcgaagag gcccagggcg gtgagcgtgt agaacgggac gcagtacggc
     8701 gacaacgcca ccaggaggtt gctgtcgctg agggacacct cgccgccttt ggcggaaact
     8761 ttcatggatt tcactcggaa ccccgacagc cacgcggcga gggcgtgggt cagttcgtgc
     8821 ccgaacacgt agagccgcat gggtttttgg aacagcaggt ggaggaggac gtatccgccc
     8881 gcgccggcca gcacgggcgt gaggggcaac cgtttcccgg ccagttcgca gaggagtcgc
     8941 cacgtggtcc agagggccgc cgccagcagc ggcagaaacg ccgcggccag gaggggggag
     9001 gtccacgagc ggctggaaaa accggccgtt ttcgaaaaaa gttttttcac gggggagacg
     9061 ggcgtcagcg tccgccggaa ggcggaacga gccggggttc cggcagatct ttccagccga
     9121 agggccgccg tttcaggaac cggccgtggc ccttcgggcc gacgaatttc ccgttccgca
     9181 ccaccggttc gccccgcgac agggtgtgca cggggaaccc gcccatcgtc aggccctggt
     9241 agggggacca gtcgcagcgg gagaccagtt ccttggagtc gatggtgcgg acggcgtcgg
     9301 ggtcgaacat cgcgaggtcg gcgtcggacc cgacggcgat ggcgcccttc tgcggataga
     9361 ggcccatgag tttggcgggg ttggtggaga tgagggacac gaaatggttc atgccgaacc
     9421 gcccggcttt cacgccgaag gtgtagagcg ccggcaggag cgtttccacc ccgggcaggc
     9481 cgtaggggat cttggtgaag tctccgttcc aggcgtcctt ctgtttcgtg gtgaaggggc
     9541 aggtgtcggt ggagaccacg gcgatggtgc cgtcggccag cccctgccag agccgttcgt
     9601 tgtcggcttt ctttttgatc tgcgggcagg tggcgtacca atggccggtt tttttgtcct
     9661 tgaaaacctc gtcgttcagc aggaggtact gcggacaggt ttcgacgtgg gcgttgacgc
     9721 cgcgggcctg ggcctgtttg aaaaggtccg ccccgccgcc ggtggagagg tgcaccacgt
     9781 agagatgccc gccggtggct tcggcccatt tgagggcccg ctggacggct tcttcctcga
     9841 tgaaattggg acgggagagc gcgtgggccc aggcgccgag gcgttccttc tgcgcccggt
     9901 gttcggcgat gaggaggttc atcacgcgct cgctttccgc gtgcacgcac acggtggcgc
     9961 cgaggccgcg ggcgaggcgc agggccgaga agatgtcggc gtcgtcggac tgccagcctt
    10021 cctgttcgta gatcatgaac atcttgacgg acgggacgcc gaagtccagc agccggcgga
    10081 ggtccgcggc ggggtccttg aggcgtttat acgcggagat gccggcgtgc aggccgaaat
    10141 ccacgcaggc ttttccctcg gcttctttca ggcgggcctc cacgccggcc aggagggtgt
    10201 cgtcgcgcgg gccctgggtg gagaagtcga cgaccgtggt gacgccgccg caggcggccg
    10261 acttggtgcc gttctcgaaa tcgtcggcgg agacggtgcc ggccacgcgg agttggaaat
    10321 gcacgtggac gtcgacgccg ccggggatca cccacaggcc cgaggcgtcg agcacctcgg
    10381 cgccgtccgc cggaaggttt tttccgatgg cggcgatctt gccgtctttg atggcgacgt
    10441 ccgcctcgcg gctgtcgtcg gcggtgaaga cctttccgcc ttttacgatc cggtcgaacg
    10501 acatgaggat tcccggggga gggagagggg gtggtggttc agcggccgaa cccggcggcc
    10561 ttgaggattt cgtgggcttc ccagaacttg tcgcgcggca cgaggatctt caggccttcc
    10621 tgggggagcc ggccgtgggc gggggcgtcg tagaccttgg gctcgatgcc tttggaccgg
    10681 aggagttcgg cggcgcgttc ggattctttt cggccgcggg tggaatagac catcagggtc
    10741 cccggctcct cgccgatctc caggtccagc atgtattctt cgaaggggat gttcttggcg
    10801 cgcagttcct cccgccagcg ttccttggcc gccggggcca tcaggcgggc ccggcgcacc
    10861 catttcgatt ccggcgggct gaagagttcg taggtgtcct tgaaccactg gtaggcgtat
    10921 ttgagggacg attcgccgac gacctggtcg cggtcgaact tcagcacttc caggtcctgg
    10981 tcgtagtcct cctgggagat ctcggccttt tcgcggcgtc ggtcgagttt ctttttctcg
    11041 cgcaggaatt tttcctcgta gaactgtttc ttttcgtcgg ggttctgcgc ccagcgcaga
    11101 gaggtctctc cctggcggta agcctcccgg gcgcgcgcct ggacgtcgat gaagaccatg
    11161 agataggcca ccgccgccgt gcccgccacc aacagggtgt tttggaccca ccgtttttga
    11221 agccactcct tcatgggatt cctccggata gttgagatgg tcgccgcgtc ggagccgtca
    11281 ggccccgatg atggtcttga gtttcgccac gaactccttg gatcggatgg gtttggtgaa
    11341 cacttcccgc acgttcttct ggctctgcac catgcggcgg gtggattgac tggccgtata
    11401 cgcgctgacg atgaccaccg ggattttcgc cgtctcggcg tgttcgttca tccgtttgag
    11461 gatcgtgaac ccgtcgatct tcggcagcat caggtcgagc accaccagga tggggaggtg
    11521 ggactggatc atgtccaacc cctcctgccc gtcggcggcc ggaatgacct catacccctg
    11581 ttgttccagc gtgaacttca tgaaatcaag gatgtaatgg tcgtcttcga cgatgaccac
    11641 cacgattttc tttcctgttg tcgaaccgtt tgtgtccatg gtgtctccct tttttcccgc
    11701 gggggttatg atgaaaaccc cgtcacttcc gatgtcccgg ttttgtccgg ggcgctgggc
    11761 cggctcacag ggcgatcttt tggaacgccg cggccggccc ggaccaggtg caaaattcgg
    11821 gatgcaacag atgggccaga agctcgatcc cgtccaccac gcgcgggccg ggccggttga
    11881 tgtaggcgtc gccgtccacc acgtagagcc ggttgtcccg gaaggcgccg atttttccag
    11941 ggaaagggca tttggccaga agagccgcct ctttcatcgc gccggccaga tgatgcccgc
    12001 agggcgcgca gacgacgacg tccggattga actccgccag ttggtcccag gtcatgggga
    12061 gggaatcctt tccggccgac gccggaccgg gctttccgcc ggcgatctcg atcatctccg
    12121 ggacccacag gccggccaga tagggcggat ccatccagtg cagcacggcc acgcgcggga
    12181 tctgtttgtc ccgataggcg ttgcattggg cggccatggc gcggagccga agatcgtaga
    12241 cgatgtcctt ggccttgtcg agggtgccgg tggtgttgcc caccgtgcgg aagttctcga
    12301 agatgtcttc cagggtgcgc ggggcgagat aaagggtttt ggagacgggg gggatctctc
    12361 cgtcggtggc ggcccgggca atgacgacat cgggtcggag ttcgttcagc cggcgcccat
    12421 ccacccgaag cagtttttcg cctttgtgga gtctgtcgga aacgtgccgg tccagccccc
    12481 ggctggacag ctcggaaagg tccaacgcgt ttttaagcac caccggtttt gtcttcactt
    12541 ccggaggata gtcgcagtcg ggggacacgc ccaccagatg gtccgtcagg ccgagagcgc
    12601 acaccatctc cgtcccgctg ggaagaaacg aacagattct catgacgatc gccttttctc
    12661 ttgcaacatc aagttacaaa tgaaacaacg ccgactttcc gaggccgaaa cacagtgagg
    12721 gtcttgggaa gtcggggtta aaaaaatcac tcagcccctt attttatctc tgtagggtcg
    12781 cctcgtccat agtttttccg gggggcggtg atatataata agggaggacg cgagggggaa
    12841 aatgggcaag aaactttttc tgttcgatct ggacgggacg ctggtgacca ccggcggcgc
    12901 ggggctgcgg gcgctggacc gggcttttga aagtcttctg gggctgagaa acgccatgga
    12961 gggcgtttct ccggccggca agaccgatcc cctcatcgtg cgcgaggtgt tcgtggagaa
    13021 actcggccgc gtcccgtccg ccgacgacac ccgcgccgtg tgcgagagct atctccgcta
    13081 tcttccggag gaggtcaaga acgggtccgg ataccgggtg atggcgggcg tgcgggagac
    13141 gttgcgggcg ctggccgcgc gggaggagat ggtcctgggg ctgggaacgg ggaacctgga
    13201 acgcggggcc cggatcaaat tggccccggc cggtctgaac gattactttc ccttcggcgg
    13261 gttcgggtcc gacgccgagg cccggcccga agtgttgcgg gcgggggtgc ggcgcgccga
    13321 ggcgcggtcc ggggaacgct tttctccgcg ggacgttttc gtgatcggag acactttgtt
    13381 ggacgtggag gcgggaaaag ccatcggcgc caccacggtg gcggtggccg ccggccatgc
    13441 cggcctggag gacctccgcg ccggaaatcc ggacatcctg ctggaatcct tggcggcgga
    13501 gcgtttctgg gaaggcctgc cccgttgagc cggaggaaga aaaaccgggg atttccgggc
    13561 cggaaaccgg ggacggctgt tttcaaaaaa gagtggaatt ttgagcgtat ttcatgcatg
    13621 cttaggatat ctgggcagcc cactcaacgt gagggaagga tgacaacgcc atgatcgcgt
    13681 tcggccgcgg catttgcggg agattgtccg aatccggcga aaaagaatgg ctcgaaacca
    13741 acgggcgtgg cggctatgcc atgggcacgg tggccggctg caacacccgc cgataccacg
    13801 gtctcctgac cgtgccgctc cgtcccccgg tccaacgttt tcaggtcctc aaccgtttgg
    13861 aagagtccgt gttcatcaac aacacgcgct accccctctc ctgccaggcc taccccgggg
    13921 tggtgcaccc ccacggctat cattatctcg agcgtttccg tctggacccc ttccccgtgt
    13981 ggacctacac ggtttccaac gtccggctgg agaagacgtt cttcctgcga tacggcgagg
    14041 acaccgccgt ggtgctgtac cggcatctcg ccggcccgaa gatcgccatc gagacccgcc
    14101 ccctggtgac gtgtcgcgac catcaccaca tcgtccggga ggacgagcgg ttcaaggacc
    14161 gcttcgagat atcgccgcac ctcgtgaaat tgaaatgcac gtcggaccac acgctcttca
    14221 tgggcgcgga ggaggggacg ttcaatcagg acagtttttg gtatttgaac caggaatatg
    14281 agtgggagaa gatgcggggg ctggaacacc gcgaggacgc gttttccccc gggctgttcc
    14341 gcttcgaact ggacctgggc cggacggtgg cgctggtttt ttccactcag gaacgcaaat
    14401 ccagcgaggc gctcgactgg agccggcagg aacggctcct gcggaaacag atcctggaca
    14461 aatcggcggt gcgcggccct ctggccgacc ggctcgtctt ggccgccgac cagttcatcg
    14521 tcgaccggga caaaggatcc tccgtcatcg ccggctaccc ctggttcgag gattggagcc
    14581 gcgacgccat gatctccctg cccggcctgt gtctggccac cggtcgaacc gacgaggcgt
    14641 gggagatgct ggaactctat gccgcccacc tcaaggaagg actcttgccg gtgcgtttcc
    14701 cggacgccga cgcccccgtg gattacaacg ccgtggacgc gcccctctgg ttcatcctgg
    14761 ccgtccagaa atatctgaag gccaccaacg acacggaacg ggtgaaacgt cttttgccgg
    14821 ccatgcggca gatcgcggat tcctaccagc gcggcaccca gttcggcatc cgaatggacc
    14881 aggacggtct catcgccgcc tccaccgacg gccaggccct cacctggatg gacgccaagg
    14941 tggagaacac gccggtgact ccccgttccg gcaaggcggt ggagatccag gcgctgtggt
    15001 acaacgcgct tcaatttctc acggaactgg acttcaagct gaacgagccg tcccgcggat
    15061 acgacaagct ggccgccatc gcgcgggcca gtttcaacga gaagttctgg aacgaatcgg
    15121 ccggctacct ctacgaccgc atcaacggcc gcgaccgcgc cggcgccgtc cgccccaacg
    15181 ccctgttcgc catcagcctg ccgtacgaga tcctggagga gtcccgcttc cgcccggtgg
    15241 tggacaccgc ctggcgcgaa ctgtacacca catacggtct gcgcaccctc gctcccggcg
    15301 accccaacta caaagggaag ttcgaagggc cccccgaaga ccgcgatgcc gcctaccatc
    15361 aggggtcggt gtgggcgtgg ctgatgggga gttttctcac cgcctacgtg aaggcctacg
    15421 gcccgtcgga aacgaccaag gcccaggtgg acgagttcct gcggccgctg ttggaccatc
    15481 tcaccgaagg gggcgtgggg agtttttccg aggtcttcga gggggacgcg ccgcacgcgc
    15541 cgcgcggctg cccggcccag gcctggagcg tggcggaaat cctgcgcgtt ttgtgggaag
    15601 agggggtggt tgtctgagcc gttcctcctt tcggtcgcgg gatcgggaca acggaagccg
    15661 ctggttcgcc ggcttctttt ttttggcttt ctgcctcgcg gcgccgtcgt cctcggaagc
    15721 gctgcccggc ttccggaatt tctttcgcgt gaagaccgcc taccgcaccg gatgccaggt
    15781 gtgccatcag ccgcgggaca accgcctgac gccgtacgcc cgggaatacg tccggctgaa
    15841 cggcgggttc gccgccctgg acgccctcga cgtcatggac ccggacaagg acggttttcc
    15901 gtccaaagaa gagctgggtt ttcactccaa tcccggcgac gcccgttcca cgcccgccca
    15961 catcggcgac tggctctcca acctgacccc gtccgagccg ccgcgggaga tcctcgtcga
    16021 cctctatggg aggatccctc cttatgaggt ttttgaaagg ccgttgaagc gccaccaggc
    16081 ccagtccctc gaacgggctc tcggacagaa actccgggat gaggaacttt ttcccgtcat
    16141 tttccttttc aataagaagg gggaagcggg agcccctccg gctccggacc ggggcgccta
    16201 tgcgttctgt tgggggaaga agatgacgtc cctttttgcg gtcgtggcgc cggagggcac
    16261 gctgcgggcg gtgcgttttg ttcggctgta tggggaccag cggttcgaca acgaacagta
    16321 tttgagccag ttcccgggaa ggacggcgga gacgttgcag ggggtgtctt ccccgcgggg
    16381 ggccgaggcg cagaacgacg acatcctctc ggcgctgcgg cggtgcctcg agatcatgga
    16441 cgtggcggcc cccgtgtccg tgacgccggc cgccctgaag gacgtctcgc cggcggagga
    16501 cgacggttgg tattatcggc cgcgcccgag cgagccgctc ccggaatatc cggaaccgcc
    16561 gttgaaaccc ctgcccatcg ccccggtccc tccggccccg gccgaggagc cgaacggcgt
    16621 ttctccggaa aacgggacgg accaggaacc cgcgaccgaa ggggccaatc caaacacgga
    16681 ggatacgaat ggtcggtgac atcgttggcg tcggcgccgc gtttctggcg ggggttctga
    16741 cttttttgag tccctgcgtt ctgccgttga tcccggtctt tttgacgttc gtgacggggg
    16801 tctcgttcga ggagctttcg gcgtccgcct cgccggacgg cggcacgcgc tcccagcgcc
    16861 gcaccaccct gctccacgtg acggcgttca tcctgggttt ttccttcgtg ttcgtcctcc
    16921 tgggggcgag cgccacgttc ttgggacggg tcttgttttc ccaccaggag tggttcctcc
    16981 gggggggcgg ggtgttgatc gtcctcttcg gcttcttgat gatgggactt cttcccgtgg
    17041 ggttcctgca gaaggatttc cgcgtgcacc tgcgccagaa accggcgggg ctcgccggct
    17101 cgttcctcgt cggcgccgtt ttcggtttcg gctggacccc ctgcgtggga ccgatcctcg
    17161 gctccatcct tctcctggcc agttcgtccg actcggtgcg ccagggcatg ttccttctcg
    17221 ccgtgtactc gctcggtttg gctttgccgt ttttggccgc ggccttgttg ttcaacgggt
    17281 tcctgcggtt cttccggaaa atccgatggg tgatggtctg gctggaacgc gcggccgggg
    17341 cgttcatggt gttggtgggc gttctggtgg ccttcgggca gttttcccgg ctgacgggat
    17401 ggatgctcca ggcttttgag ccgtgggtgg actggctcat ccgtctgggg atataggagg
    17461 ggggaagatc atggcaccca aagacgttta cgacgtcacc atcatcggcg gcgggccggt
    17521 cggcctttac gccgcctact acgccgggtt ccgcgccctg cgggtcaaaa tcatcgacag
    17581 cctcgccgaa ctcggcgggc aggtcacggc gctttatccg gagaaaatga tccgcgacgt
    17641 cgccgggttc cccggcatcc tggggaagga cctggtccgg aacctggcgg aacaggcctt
    17701 ccaatacaaa cccgtcatcg ggctcagcga acaggttcag gaactgacgg tcaccggcga
    17761 taaggagatc cgcctcctca ccgacaaggg cgaacatttc acccgcgtgg tcctcatcac
    17821 cgcgggcatc ggcatgttca cgcccaggaa actgccgctg ccggaggcgg acaggttcga
    17881 gggcaagggg ctgttctact gggtgaaaca cctcgaggaa ttccggggga agcgtatcct
    17941 ggtggtgggc ggcggcgatt cggcggtgga ttgggtgctg ggtcttctct ccgtggccaa
    18001 ggagatcacg ctggtccacc ggcgcgacgt tttccgcgcc cacgaggatt ccgtccggaa
    18061 gatgatggac tcctccgccc gcatcaaaac gtttcacgag gtgaagtcga tcctcggtcg
    18121 ggacgccatc acgggcgcgg tgatcttcaa caacaagacc ggacaggagg agacggtctc
    18181 cgtggatacc gtggtggcct gcctcggttt cctcgccacc ctgggaccgc tggagacgtg
    18241 gggtttggaa ctcgacggcc gcagcgtgaa agtgaatttc cggatggaaa ccaaccggcc
    18301 gggcatctac gccgccgggg acatggcgtc ctatccgggc aaggtgaaac tcatcgccac
    18361 cgggttcggc gaggcggcca ccgcggtcaa caacgccgcc gcctacatca accccaagag
    18421 cacggtgttc ccggggcatt ccacggagaa aaaacaatca tgaacgcgag acatcggttt
    18481 tttgcggcgg cggtcctctg ggggagcttg ggcggcttcg cggcggctca ggaggagata
    18541 acgcctcccg tcgacgaaca accggaatcc gcgaagattt tcctgcaggc ctggccgatg
    18601 gcggacctgc gccgggaacc gtcgccgccc atggacaacg ccgccttgaa gagcccctac
    18661 gacgccgatc cgttccagga aacacagctc ctcttctgcg aggaggtgcg cctgcgggag
    18721 gagtccgggg cgtgggcacg cgtggaggcg gtggaacagc ccgagttctc ccacgcccag
    18781 gcgtggggcg gctatccggg atgggtcctg aagaaatcgc tcgtgcccaa accggcgggt
    18841 ttttccgcga acgccgtggt ggccgtgaaa tacgcccgac tccatgagga gccttcgaaa
    18901 aaatccgggt acgtggagat tcccctgggg tcccgcgtga gcgcccagcc gtcggccccg
    18961 tgggccctca tccgtcttcc gaacggcggc gaaggatgga cgtccctcaa gagcctccgc
    19021 tttttcgaga acctgccgaa agacgacggc gcgctccgca aggcgatcgt gaagaccgcc
    19081 gaactgttcg tgggagaacc ctattattgg ggcggccgct gctcccaccg gacgaaggtc
    19141 ctcgaccggc ccaccggggt ggattgttcc tccctggtga acctggccta tcgcgtcaac
    19201 ggggtggacg tgccgcgcga cgcgcatgaa caatatttga agtccctgcc gctcgaacgc
    19261 cgccagatga aaccggcgga cttgatcttc ctggcgaagg ccgacgatcc caaccgggtg
    19321 gtccacgtga tgctttccct gggggacgac ctggtgctgg aggcggtgca ggagttcgac
    19381 gcggtgcgcg tcaccaccgt caagaacaag ctggggaaat cgctctccga aatatcgccc
    19441 ctcgaacccg ccggccggta tgtggtctac ctgggccggc tcctgccgga ccccgccgcc
    19501 gcggccgctt cgccggacac tccccgctga atttgcagga aaaattccgc ctcagcgtcc
    19561 tgcgcgtcgt tccggtccgc tggcccctgc gccggccgtt cgtcacctcc ctgggcgcca
    19621 aatcgtattc ggacaacgtg cgggtgatgg cccggttgtc cggcggcgcc accggttacg
    19681 gcgaggcctc ctcgtccctg gccatgccgt tccagaccgc ggcgaacatg tcggccgcgc
    19741 tgcgccgcct cttccattcc ctgcgcggct cggacgcgcg cgacatcgaa aagatcgttt
    19801 ccgcggcgtg gcggttggag gggaaatatc ccaccgcggt ggccgctttc gagacggctc
    19861 tctgggacgc cctttcccgc gcgctcggca tgcctttttc cgtcctgtgg ggcggcgctt
    19921 ccacgacgat cgagacgttg atgagcgtct ccgccgtgga cccggaagaa gtatccgccc
    19981 gtgttcgcgc cggccggcgg agggggtttc ggctcttcaa actgaaactg aacggccacg
    20041 aaccgccgga acgcgaccgg gcccgcttgc gggccgccca tcgcgcctgc ccgcgcggcc
    20101 ggttcctggc cgacgccaac cagagttatt ccccggagaa tctttcggcg ttcctcgccg
    20161 cggctcggcg ggatggcctg tccgtcgagg tgctggagga accgttcaaa aagaggagct
    20221 gggccgcgct cgcttccgtc cgtgcaacgg tgaggacgcc cctcctcctc gatgagtccg
    20281 tccagacgcc ggccgacgcc cggcgcgccg cccggggccg gctcgcccgg ggggtgaacg
    20341 tgaagctcgc caagagcggg cttctccgcg gccaccacat ccttcgtgaa ttctggggcg
    20401 aagatgaacg cttccgccgt tccgcggcct cgccccgccc gctcttcatg atcggctgca
    20461 tggcggaatc gaaactcggc ctggccgcca gcgtccattg ggcctgcggg ttgggcgtgt
    20521 tcgattacgc cgacctcgat tcggacctgc tcctgaaacc cacgccctgc cgcggcggct
    20581 atgtccgccg cggaccgacc ctctcgcttc cccgccccct gccccccgga ctcggcgtgg
    20641 agcccgcgtg atccgctctc ccgcccttct cctcgccgcc ttcgtcggag tcgtggccct
    20701 cgtgctccac gcgcggcgtc ggggcgcgtt ccaggcggtc ttccggtttt tgccggttcc
    20761 gttctggtgc tatcttctcc ccatgttggg caccaccgcc ggcctttttc cgtcggacgc
    20821 gccgctctac ggggccttgt cccgctacct tctgccgttt tgcctggccc tgcttctggt
    20881 gagcgccgac ctttcggccg tggcccggtt gggcgcccgg gccctgggcg tgatggcttt
    20941 ttccgtcgcc ggcatcgttc tgggggcggt gttcggtttc gtcctgttcc aggaccggct
    21001 tcccgaagac gcctggaagt cggtcggggc cctttcgggg agttggatcg gcggcagcgc
    21061 caacatgctg gcgctgaaag aggcgctttc cgtgccggac gcgatcttcg ctcccgtgat
    21121 cgtgacggac accctgctgg cctatgcctg gatggccttc ctcattgcgg gcgcggggtt
    21181 ccagaaaaga ttcgacgcct ggaacgccgc cgccgttccg acgttcgggc tcgccggcgt
    21241 ttcgcccgct ccgacggccc ggcccggttt ccgggcggcg ccgctcctgg cggccatcgg
    21301 cgcggcggga ttctcgattt ttttgggcga ccggcttccc gccctcggca cggcggtgac
    21361 ccacgccacc tggacggtgt tgatcgtgac gtctctgtcg ctggccctgt ccttcgtccc
    21421 cgctttttca acggccggcg gccttacgga aaaatggggc gcgacaattt tgtatattct
    21481 attggcttcc atgggcgcgc gggcccgctt cacggacatt tttcagtcgc cgcggttcct
    21541 ggccctgggc gtggtgtggc tggccgtcca tgggctggcc cttttggcgg cggcccggtt
    21601 tctgcgcgcg ccgttggcgc tcctcgccac cgccagccag gcctgcgtcg gcgggccggt
    21661 gtcggcgccc atcgtcgcgg cggtgtatca cccgtccctc gccgccctcg gcctgctcct
    21721 ggcggtgttg gggaacgtgc tggggtccta tgtcggtttt ctcaccgcgc atctttgccg
    21781 ctgggcggcc ggatgacgga tttcgtggag aggggaaaat gtccctgaaa aaaatcgtcg
    21841 ggttctgttt ttgtttggcg gcggtcgccg gcgtgccttc cgcgctctgg gcgcagaaca
    21901 tggacatgct cccggacatg tccgtggacg atctgttgcc cccgccaacg gtctacgagc
    21961 ccgttccgac gatgcccgaa gcctggggcg ctccggcgcc gctgcgcctg gtgcatccgg
    22021 cggaaggcgc gcgcctgcct ttcctgaaat cctctttcgt cttcggctcc gccgacccgc
    22081 agggaaaatt gtccctcaac ggccagccgg tgcccatcca tcccgagggc gggttcctgg
    22141 ccatgatccc gtattccacc ggcacttttt ccatccgggc cgaactggtc ctgccgaaca
    22201 gcacggtcat cgcggtgcgc accgtctccg tggccggccc cacgctggcc tcgccgcgct
    22261 cccccttgac ggtggaatac gtccggccgg acctcgacac gacggtccgg cccggcgatg
    22321 tcgtgtcggt gatctgcaag ggcagtccgg gcatggaggc cgtttttcag gtacaggggg
    22381 tccacccgcg attccccatg atggaggacg cctccgccgg cgtgaagggg gtctatcgcg
    22441 gcatgttcgt cgttcagccc ggcgacgaat tgaacgacaa ctggatccgc gtgacgctga
    22501 ccgacaagga acgcggccgg aagatcgtcc atgagtcgcc cggccgcctg agccggctca
    22561 acgaccgcgt cccctgggtg gtggccgtca ccaccgacaa cgccgtcgtc cgcgccgtcc
    22621 ccgccctcgc ctccggcgat aagggcggct acctgctctt ccctccgccg ggcacccgcc
    22681 tgcaggtcct cggccggcag ggagccgaat taaaggtgaa gctgggttcc tcgcggaccg
    22741 gctggatcag cgaaggcgac gtgcgccgac tccccgacgg cacgcccccg cccatcacct
    22801 tggccgggac cgtctccgtg gcctcggacg gcgcgcgctc gacgctggtg cggctcatcc
    22861 tgaaacaaaa ggttccgttc ctcgtcgagg cgtctcccga caaaaaacag ctggacattt
    22921 ccttcttcgg cgcggcctcc aacaccgact ggatccatta cgcctccacg ggcgccgtgg
    22981 aacggatcca atggttccag gacgatccgg aaacctaccg cctccgcgtg cacgcgccgg
    23041 ccggcgactg gtggggatac gacacccgtt acgaggggac cacgttcgtc cttgagttgc
    23101 gctcgccggc gcctccggcg gcccaccagc cgccgctcgc cggattgcgt atcgccgtgg
    23161 acccgggcca ttccgccgat cgcggttcca tcgggccgac ggggctgttg gaaaaggacg
    23221 ccaattgggc catcgcccaa tgcctgaaga aaaaactgga ggcggcgggg gccgaggtgg
    23281 tttttatccg cgaggggagc cagaacgttc cgctctacga ccggcccaaa ctggcctggc
    23341 aggcccgggc cgacctcctc gtcagcgtcc acaacaacgc gctgcccgaa gggggggacc
    23401 ctctggagaa gaacggctac agcgtctact attttcaacc gcacagcttt gaactggccc
    23461 gggagatcca ccgggcctat cgggacatcc tcggtgcttc ttcccggccg gccgccctgc
    23521 gggacgacgg cctgcattat gggaacctgg cgctggcccg gccgccccaa atgcccgcgg
    23581 tgctcaccga gtcggcctac atcattgtgc cggccgagga ggcccttctc aagaaagagc
    23641 cgttccagtg cgactgcgcc gaagcgatgc tgcgggggat cgttcgtttc gtgtcctccg
    23701 cgcgcggcgg accgtcgccc cttccgaaga attccaggga caaacgctcc aagtcgaact
    23761 gagggtcgtc aggtcgttct cttccggcga tggatgacga cgcggtcttc ccgcgtcatc
    23821 gtgtggaaca ggtgttcgtc gggggtttcc cggttctcgt aggggatgtc cggaaaatcg
    23881 atcgtgattt tttcatagtc cggccacgcc accttctcca gaagatagtt tttcacctgt
    23941 tcgtccttgt cgctgaccgt gtgcttccgt aggatgatcc aatccgcttt cagggccggc
    24001 gagaggtctt ctccggtcag cccgcccacc acgcgcatcc gcgtgtagaa tttcaacggc
    24061 aagtcctcgt aggtgatcgc cacgacgtcg ctgtccctgc cattcttccg aagatagtcc
    24121 acgatcccgt ccatcggtcc cttgtaacgg tgagtgatct cgtagaggaa cggcggcaac
    24181 cccccgtaga cggccaggcc catcagcagc gcggcggcgg gaacgatccc ccaaacgtcc
    24241 tgcagaaaac ggaggatcca tccttctccc gcgcaggcga gcgggatcag ggccgccaga
    24301 tatcggaaat agttcgccgg actgagaacg gcgagaaaaa cgaacgtgac gccggcgacc
    24361 atcgacagga gagccgcgga cgaacggtcg cggggcgaga aggacggttt tcgtccggtc
    24421 atcaccacgg ccagaagggg gaacaggagc aggagaaggg ggaaaatatg ttccagcgtt
    24481 tcggaggcgt agtaacggaa gaaatcgagg gctcgcccgg cgttgaagag ccccacgccc
    24541 cggtccgttc cccaaaacag ccacaccgcc ggcaggttca cgacggaaac ggcgaggcac
    24601 gccgccagga ggggcgggaa ccggtcccgc gagaaaacgg acgcgtggag cagaagggcc
    24661 gccaggaggg cgccgcagag gacgtaatgc gaatggaaca ggaagaagga ggcgcagaag
    24721 aggaggagga atccggactt gtttttttcc gtcaagcggt cgtaggcgta gagccccaga
    24781 agggtaaaaa acgccgccgc gctgtaatag cgcgcctcgc gcgccagaag caggaacggc
    24841 accgagaacg tcagcgcggc ccccgcccag agggcccgcc gcgggtcctc ccaacgccgc
    24901 cgcacgaaca gcatcaacaa cgccaccgtg gccacgccca gcagggcgaa ggggagtctc
    24961 gccgaaaaag tcccgacccc gaacaccgcg aaggatcccg cacacaggta gaactggagc
    25021 caggggtgcc agcgccatag aaaatccttg gtgtagtcgg ccccccggtt ttgggaaaag
    25081 acattttttc cgtcggtgcc gaacggcagt ccgcgcgcaa ggacggtttt cgccaccagg
    25141 gccgtttcgg actcgtcttg ccagaggatt tcgcggtcca agccgtgaag ggaaaagaac
    25201 gccgccaagg ccacgatgag gactcccccg agggtgtgtt tatcgagccg gcgcataaaa
    25261 cggacggcgg tcagcaggga gaggaggcct gtccccgtcg ggtccgccgt tcggaaaaaa
    25321 agtaaagagg gatccccagc ccggcgacgc cgaccccgag aagggattcc ttcggccggc
    25381 ccagaaaggc gttgatggca aaggcgaggc acatcaggat gaacaccgtc ggcgtccacg
    25441 gatatcccca cacccggtag gcggaggggc ttcgctgggc ggcgcggcgc cggaagagga
    25501 acagggagag tcccgtgccg gcgaagaaga ggtagatgac gatttacgtg taggtgacga
    25561 actggtccag agtgcccgtc cacaccagca acgtcgcgag ggccgccgtg gtcagaaggg
    25621 acatcacggg ggtgcgcgtt ctcggcgaga cgagggcgag ctttccgaaa agggcgtggt
    25681 cccgcgccaa ggcgaagagg atgcggctac ccgtcaggat gtagccgttg agcgcgccca
    25741 gggtggaggc catcaccatg gcggccacca cctgaccgcc ccgcccgacc catattttgt
    25801 ccatggcccc ggccgccacc aggtccgtct ctcggagttc gggcaggggg aggtaataaa
    25861 ggtagaccgc gttcaccacc aggtacagca ccgtggtccc cagcaatccg aagaccaggg
    25921 ctttcggcag gttccgttcg ggttcgcgga cctcttccgc cacgtacgcg gcgttttccc
    25981 agccgccgta cgtccacaac gcgaaaatca tggccaggcc ggtggcgtgc cagatgttcc
    26041 aggaccactc cggccagaaa ggctggaagt tctccgccga ccctttcccg gcgaaaagtc
    26101 cgacggcgat gatccccagg agcgccagca ccttgagcgc ggtcagaacg ttctggacgg
    26161 ttttgcccgc ccggagtccg acgatgttgg tggcggtgag cagaacgatg ccgccggtgg
    26221 cgagcgattt gacggcgccc gggccgccgg cgagttcggt cgcgcggcag gtatgttccg
    26281 caaaaacgaa cgcgaggatg gagatcgtgc ccacgcgctg cacgaagagt ttcgtccagc
    26341 cgaagacgaa gccccaaagg cgcccgtagg cgttggtgag gaaaacgtag tccccgcccg
    26401 tcttggggaa cagcgacgcc agctccgcgt aggtcagcgc gccgcagagg gagagcagcc
    26461 cggcgagccc ccaggccagc aggaacatga agccggagga ggaatgcccc gccacggaat
    26521 cggagaggcg gaatatcccg gcgccgatga tgcatcccac cacgatggcg ccggcgtccc
    26581 agacgttcag cccccggcgc agggcgggtt gggtttcgtt gttcatggcg cccatagact
    26641 accaaagtcc cccgggacct ggccagccgc ccggtcccgc ggggcgcggc gcggccgggg
    26701 ggttgacaac gccgtcttcg atatgagaaa atagattttg agacgccata tcaaaatgaa
    26761 cgatcacctc aaggccttcc ggaactatct gaaagaacag gggctcaaaa acacctctca
    26821 gcgggaggag atactcgctt tcctgggaag atcttcacgg cacctctcgc cggaagagat
    26881 cttccgggaa ctccggcgac gggacccccg cctcggccgc gccacggtgt tccgcaccct
    26941 gcgccttttg ggcgactgcg gcctggccac gcgggtgacg ttccccgacg gtaagcagaa
    27001 gttcgagacg cgtctcggcg gtcatcacca cgaccacatg ctctgcgtgc attgcggccg
    27061 ggccatcgag ttcgtcagcc cggcggtgga agaggcccag aaacggttgg cccggcggta
    27121 cgggttcaag gtcctgtggc accgccatga gattttcggc cgctgccgcc gctgcgagac
    27181 ggcggggccg tctccccggc gttcctgatt tttttgtgtt tttattgaga taaagtctca
    27241 attaagaccg gcgcccgccg gtcgcatgtc caggagggat cgatgggagc acgacggaag
    27301 agagcggcgt tgtttttggc ggcgacggct tttgcggctt cggccggggc ggaggccccc
    27361 gcaaaaattc aggacaattc gttccttctg gaggaggcct ataaccagga accgggcgtc
    27421 gttcagcaca tccaggcttt ccagtatctg acgaaggaca agacctggac cgcttcgttc
    27481 accgaggagt ggccgtggaa ggggcaaaag aaccaacttt ccgtcacgtt gcccgtcgag
    27541 cattttcccg cgccgggcaa cgacaccggc ttgggagacg tgggactgaa ctaccgttac
    27601 caggccctct tggacgaccg cgtggcggct tccccgcggg tcactctcct tctgccgacc
    27661 ggcgacgagg aggatggttt cggcgccggc gcgttgggat accaggtcaa catgccgttc
    27721 agcgtggacc tctcggaccg atgggtgacg cactggaatt tcggcgccac ctacaccccc
    27781 gacagccgcg ccgccggcag cgtccgggcc gatacgctgg gtttcaacgc cgggttcagc
    27841 gtgatccacg tcctctccga tcggctcaac ctgatgatgg agaccgccgc cacgtcggag
    27901 gaaacgccgt tgccggccgg cgggacggag cggaacgaca cgttcttttt gaaccccggt
    27961 gtgcgggggg cgctgaactt cgagtcgggg ctgcaggtcg tccccggcct ggcgtttccc
    28021 atcggcgtcg gcccgtccga cggcgaacac ggcgtgttcc tgtatctttc gttcgaacat
    28081 ccgctgcgtt cggggggttg agccgcgggc ggccatcggt cggtgaatta acgaggacgg
    28141 catggaaaag gcggcggcgg ttctggacga taaaaaagtc gtggccatga aacggctggc
    28201 cctggtgggc aatcccaacg tgggcaagtc ggtggtgttc aaccgcctca cgggacagta
    28261 cgtgacggtg tccaactacc cgggcacgac ggtggacgtc tcccggggag gcggccggct
    28321 gggcggggaa aactatgaga tcgtggacac ccccggcgtg aactccctca tccccaactc
    28381 cgaggacgaa cgggtcacgc gagacctgct cctgcgcgag aacccggacg tgctggtcca
    28441 ggtggccgac gccaagaacc tgcaccgcgc actcctgctg acgctggaac tggcggagtt
    28501 ccgccggccg atgctgctcg tgttgaatat gatggacgag gccatgtccc gcgggctggt
    28561 gatcgacccc gcccggcttt cccggcggtt ggggatcccc gtgctccgga ccgtggcgac
    28621 gttgggggag ggtctcgcgg acgtgaaggc ggcggtggcg cgggcggcgg tgccgtcggc
    28681 cgccgtgccg tatccgccgg aaatccaccg gtcgctggag gcgttggacg gggcttggcg
    28741 gacggatttt tctcccaaga gggcggtgag cttggcgctt ttgtccggcg acggttcggc
    28801 gctgacgtgg gctcatccgg gtctgcggcc cggcgaaacc gaggagtccc tgtggcgtcg
    28861 ggccgacgca cagagggcgg cttttgtccg gccgccccgg ctggtggtct tcgaggcgcg
    28921 cgacggatgg gtcaaagaga cgctggggga ggcggtgtac cgccgcgccg gcccgtccgg
    28981 gaattgggcg tcgcggttcg gaaactggtg catgcggccc tggcccgggt acctgatcgc
    29041 cctggcggcg ttgtacgccg tctaccagtt tgtcggcgtc ttcggcgcgg gggtggcggt
    29101 ggattttttg gagggaaccg ttttcggacg gtacatcgtt ccggcggcgc agcgtttggc
    29161 cgatctgctt ctgccgtcga cctttttgcg cgacatggtg gtgggcccct acgggatcgt
    29221 caccatggcg ctcacctacg cgttcgcctt gatcttcccc atcgtgacga cgtttttcct
    29281 cgccttcggg atcttggagg attcggggta tttgccgcgg ctgtcggtga tgatggaccg
    29341 gttgttccgg ctgatggggc tcaacggccg cgcggtgctg ccgatggtgc tggggctggg
    29401 ctgcgacagc atggcgacgg tcaccacgcg cgtgctcgac accgccaagg agcggctgat
    29461 cgtgacggtg ctcctgtccc tttccgtgcc ctgctcggca cagttggcgg tgatcttggg
    29521 gatgaccgcc ggcatgtcgc cgcgggtctt cctgttgtgg ctgggcgtgg tggcgggcat
    29581 caccttcgtg gtcggttggg ggtcggcgcg tttcctgccg ggcgcccggt ccaccttcgt
    29641 catggagatc ccgccgttgc gccggcccat gctgcggaac ctccttctga aaataaaggc
    29701 ccggctggaa tggtatttga aggaggtggt gccgctgttc gtgctgggca cgctggtgtt
    29761 gttcttcctg gacaagttcg gcggactgaa acggctggaa gtcctgggcg cgccggtggt
    29821 tcagaaattc ctgggcttgc ccgccgcggc cacgcagacg ttcctcatcg ggttcctgcg
    29881 gcgcgactac ggcgccgccg gactttacca tctgcagatg gaggggctga tgaacgaccg
    29941 ccaggtgacg gtcagtctgg tgttgatcac cctcttcatg ccgtgcatcg ctcaatggtt
    30001 gatggtggtg aaggaacggg gatggaagat ggcggcggtc gtcaccgcgg tggtcacggt
    30061 gattgccctg gcggcggccg gcggactgaa cgccctcctc tccgtcgcgc cccggctcat
    30121 ttcatcctaa gaaaggtgtg acaaatgacg gaagacaaga aattccccat ccccaccgcc
    30181 gagaaggagg acatcgaaga ggggctgaac gtggtgtgga acatgttcga gaagaaagtg
    30241 cgccggcggg cggagatcca ggcccgcctg accgaggccg tcggggaaaa ggccctcgat
    30301 ctgctgatgc gcgaggggct tctgcgggaa tccggcgacg aactggtttt ttcgccggag
    30361 ggggaacgcc tcgccaagga catcatccgc cggcatcgcc tggcggaacg gctcctgtcg
    30421 gacgtgctga gcatggcccg ggccgaactc gattccaacg cctgccggct ggaacacatc
    30481 atctcgccgg agatcagcga gtccatctgc accctcctgg gccatccccg gcagtgcccg
    30541 cacggctctc ccatcccgtc cggcgattgc tgccgtcggg aggaggaaaa catacgcccc
    30601 atcgtggtct ccctcgacaa catgaaactg ggcgaggagg gccgcatcgc ctacctcctt
    30661 ctgcacgacc atccggaact gcacaaactc ctgtctttgg gagtgatccc cggcgccgag
    30721 gttcgcctgc atcagacgtt ccccagcttc gtgctggagc tgggagacag ccagctcgcc
    30781 ctcgaagagg ccgtggccag gaacatctac gtccgaaaaa aatagatccg cgcgcggcgg
    30841 ttcccccgcg cgggccccct ctccgtgata caatatgccc atggacactt tgcccgtgaa
    30901 acatcagaag gtctcggagg tcctcgaacc cgagatcgtg ggcctggaac cggccgtccc
    30961 gttctggaaa cgggcggtcc ggcgggtgac ggccggcctg gcgttgggtt tcgtgggatt
    31021 ggggatgacg gtcctggggg gacttttcac cctgaccatc gtcggcgccg ggatcgggct
    31081 cccgttggtg ttcttgggga ttctggtgat ggtgttcgcg gtgtttttat ttttcgcgga
    31141 ccgctcgacg gtttccatac ggttccggcg ttgagcatct tccccgggcc gggcccgtcg
    31201 gacgccgccg aggataatct gatataatgg cgcgcgcggc ctcgtcgcga gactccgcac
    31261 cgaactcaga aaggaacccc cgctcatgaa gaagatcact ttgttcttgt ccgtcctgct
    31321 gtcggccggt ctctcttttg ccgaattcga gggcgtcctg catatcaaga tgtccgccgg
    31381 gccgacttcc gggaatggga ccatcctggt ctccaagtcg ggcgtccgca acgagctgaa
    31441 catgcagacg ccccaaaaac cggtgcgcat gacgtctctc ctgacggcca aaaatccagc
    31501 caccctctac gtcatcaacg acgaaaagaa gacctattcg gaaatggacc tggacaagac
    31561 ccgtcgggcc gccgaaaagg ccgagcgcgc ctcttataaa gtgaagcgcc tgcccgacga
    31621 gactctcttg ggctatcgat gcgcgcatgt cttcatcgcc gggtccggcg gcgaatcgga
    31681 actctggacc agccgtgaga tcatggacta cgacacgttc gccaggatga acgccgggaa
    31741 ggaccgcggc ccggccgacg gcgcctacgg gaaggccttg aaggaggccg gggcggacgg
    31801 ttttcccctg aaatccatcc atcgcgccgg aaaagagggt gtcacggtgg tggaggtggt
    31861 gcgcgtggag aaaaagccga tctcgccggc catgttcgag gtcccggcgg ggtaccaaaa
    31921 gaccggcctg ttgggggccg gcccggcggc gacgtcgatg cctccggacg ccatgaaacg
    31981 gatggaagag cagatgaaga acatgccggc ggaacagcgc gagatgatcg aaaaaatgat
    32041 gaaacagggg ggacagccgt aggggtccgg cggcgaactt cggaggcggg cgggtcggat
    32101 cagtttttag aaaattcctt caagacaacg tcggcgacat tctcggccaa gaggatgtcg
    32161 ccgttttttg tgaggtgccc gaaatccccg gcgaagaggt ccgtgaaata tttcgagaag
    32221 ggttcttttt tcaggacctc cctgaaaggt ttttcattgt cgacgaagag gaccccggcg
    32281 ttcggaccga aaatctcttt cagcggggcg gtcgaccgca acggatagga ggcgcacacg
    32341 taaacgatct gccgccgcgc cagggtttcc cacaggcgaa gatagttcct ccgcgtcttc
    32401 tccgagaagt tctggtccat ctccgccatt tcaaaatgtt cggcggcctg gatcggccgg
    32461 cccatctggg agtagacgat gcccagcgcg ccgtggacgg cggcgcgcgc ctggcgctct
    32521 gggtgggttc ggccctcgga gagcagaccc acggcctgca gacagacctt ttcggctttt
    32581 tcgtggttcc cccgcaggca gtagagaccg gccagttcga ggcgcgcgcg cagcgccccg
    32641 ggtttcaggg agatgaactc ttgcagggtt tccggggatt cgtcgaggcg cccctggagt
    32701 ttgagcgctt tggataagag gacttgggcg cgcacgttcc ggggctgggc ggccaacgcg
    32761 ttccggcacg cgcgttccat gtcggcgtgt cgtccggcgc ggtaatgcac cgtcgccagg
    32821 aggaggaagg ccggcacgct tcgggggttt attttcagga ccgcctcggc gagtttcacg
    32881 gttttggggt cgttcccttc gctgttgtaa caggcggcca tctcgatgga cgcgctttcg
    32941 gaagcggggt cgatccggag gatgtcccgg cagattttct ccgtttccgc gcacatcccc
    33001 cgatcgctga aacaatgcgc cagttccaac aaaaacagga cgttcgccgg ctcgatggcc
    33061 agggcccggc ggcacgagtc gacggcgtcg tcgaattgct gccgttcgcg ctggtggcgg
    33121 atggtcgcca gaagcgtttt catggccggg gacagtcgtt gaaccgcgtc ccccgttccg
    33181 gccgcggcgc ggaggtcgcc cggggaggag gttttccgga aacgttcccg taggctggag
    33241 gcgacgagtt ttgtgaggtg ggtgacgcgc caggaagcgg gagagagcgc gggatccggc
    33301 cggtgttcga acccgtcgtc ctcggcgtcg ttgattccca tcatggtgac gaccagctgg
    33361 ggtttgtatt tttccaggtt cttttccagt tgcgtcagta tgacggaggt cgtggtgccg
    33421 ggataggcgg cgtcaatgac ggtgaaccgg gtcttcgtct gtcggcggtt gaggatctct
    33481 tcgagatgtt ttggatagcc gccggccgtg gtggattcgc ccaggcagag gaggcggaca
    33541 tccccgcccg agttcaacgc tttgcggagg gagaggttct gttttgcccg gagcgcgccg
    33601 ccggtcagcc gcaggccggt ttccaaaaac agaagggcca gcccggcgcc cagcagaacg
    33661 aggccggttt tcttgacgaa ccgggggagg aaccgggagg gattttccat gggggaattc
    33721 tatcaaagag cggcggggcg gggtagtttt cctcggggaa ggcgccttta tgctacgata
    33781 gagccgtttt ttatgtgaac gacattcaga aaaaggattc cggggatacc gcatgagaca
    33841 ttttttcagg ttcgaagaac gggcgaccaa tttccgcacg gaggtcgtgg cggggatcac
    33901 gacgtttttg accatggcct acatcatttt cgtcaatccg gggatcctgt cggcggccgg
    33961 catctccttc cccgcggcgg tgaccgccac cgcgctgggg tcctgcctga tttgcgtgac
    34021 catgggtctg gtgacgaacc ggccgttggc gttggccccg gggatggggg tgaacgcggt
    34081 cgtcgctttt tccgtcatcg ggataaatca ggccaacgtg ccctggcagg tgggaatgtc
    34141 cgtgatcttc ttggaagggc tggtcatcct cgggctggtg cttttgggac tgcggggagc
    34201 cgtgatggac gccatcccgc tcaacctgaa gagggccatc gggatcggca tcgggttgtt
    34261 catcacgctc atcggtctca accagggcgg cctcatccgg ccggcgccca tcaccctggt
    34321 cgccttgggc gatttctccc agaaatacgt gtgggtcacg ctgattgggc tgggggcgat
    34381 actgatcttc atggcgttgc gcgtcaaggg ggccctgctg tgggggatcc tctccgccac
    34441 gggggccgcc ctgtgcctga gggtgacccg tccgccgacg cagatcgttg ggccgctgga
    34501 ttttcagacg tttttcgcgc ctttccagcg ggtcggcggc cggatggccc tgggcgatgt
    34561 tttcacgggt ctccttctgc tgaccgtgtt ctccatcatg atgacggatt ttttcgacac
    34621 catgggcacc gtggtggcgg tgggcgaaca gggcgggttc gtcgacaagc gcggaaaagt
    34681 ctcgggcatg aagaagatat tgactgtgga ttcgttggcc gccctggtgg ggggtctttt
    34741 cgggtccagt tccatcacca cctacgtcga gtccgccgcc ggggtggcgg tgggcgggcg
    34801 caccgggttg accgccatcg tcaccgggtt gttgttcggg gcggccgcgt tcttcgcgcc
    34861 cgtcattcag atggtcggcg gcgggtatcg gattcccaac gcggaccaat atcgcctgtt
    34921 cgtcaacacg gggtttcaaa ctccggcgga cgcgatcccg ccggccgggg ccggcgattt
    34981 tttcgtctac cccatcaccg ccggcgcctt gatcatcgtc gggttccttc tcatgaagac
    35041 catctcccag atcgattgga acgagttcga ggaggctttc ccggcgttcc tgaccatcgt
    35101 gggcatcccg ctgacctaca acatcagcta cggc
//
LOCUS       DAOTFD010000008        31533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_945_length_31533_cov_4.459210, whole genome shotgun sequence.
ACCESSION   DAOTFD010000008 DAOTFD010000000
VERSION     DAOTFD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 31533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31533
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_31533_cov_4.459210"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..24034)
                     /locus_tag="P5079_01215"
     CDS             complement(<1..24034)
                     /locus_tag="P5079_01215"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY28636.1"
                     /translation="MSAAVPVQADPQKVADGIGTTMASNQRKVVATDEGYWVFYTSTT
                     LTTGVPCWTYSKTGNAGSWSIPQTIFDWSGISPVNVSSMVSVWYHAPQDRVYVAAGCS
                     QSVLPANRTIFTRMGNLESNGTINWDPTGTASQARGYYQPTACTGNDPAQYAGWPVSI
                     AVDRATYVWVSANGGDDGAGIAGGNDLVTIFAKSVNPGRVDGWGGTVHYWSSQNEANC
                     APALADDSLGYKNVLVNRPGYDSVWAAGEYVPTNDTEGYWSVRNPSTEVDYDNNVTNL
                     YNDRMDDSGVNWFRDTKNVSMAVDSAGNLHVISINDVGGTTVGTLSYARILNNNSLST
                     NFDFNPISGGGDYQNCTIGISRSTCPQVADTVVVMFVENLAGAGGQLYYITGSTSVLA
                     ADWAAPASTMIQSGNSSWPNLCQYVDYPNPLPFVFVGDDGVTFDRILISNYPYPDLSS
                     MSIATSPTNAPYATKTYDLQLTNISGLVKLPTKSPVLEFIGSLGGVTNDFTMTYDVYS
                     TSIVAHLELVAPVDDGPYDIRVTNPDGKSAEAVTAYNIPGPSITSITDDNGGDLSGGE
                     NAGTEPGVIPYRHLLVQGGNYQYWSELQKSSITINNNGPGINVSSITMHGVSSFTATI
                     AIGANLPLGLWHLSVVNPDGQSVQQNSTFTVTRPTGTLTLPTAATSYAEWVDYLAGKT
                     DMMRGDGAGGIQTGAEISIRNDNSGNYLSGAGGSFTSDTEIWISTGLSAPSTWQWMFE
                     GHGGTGNDGWRTGVQVDSNSYTIKVRTVSDDFGRGPTPFLEAHVTLDRSNPINNELTS
                     PGAVSRMGDLATVEGVVQDVTSGVNSATSKLYVIHGTDYYNWDGLAGHWSPHTTNYVI
                     FTGGISAPAGNPPRGDPDCSWYWNVPGGNNFWTEGQTYQMKFAATDMLGHTSTSTVRE
                     FKWDVHAPTSTLVTPSTNTSYFNYASLPYISGTMADNLGLPLDGFAKVELAVHRSSIG
                     VTNDNSWLQSNYTWNSTPYWHTVSGSSVTPSNGSWKFLKNSFFEDRGQYEIWARAYDG
                     ANRELPSEEPPNPPTPSDAHKHRFRFDTYNPSIEIIYPSTGDAPNAYGRLISENSQWG
                     SLATLSGTASDFAQGSPVDSGVSLVEYQLYCEGADKYWDESALSGVGDWVTATNLWNP
                     ASTTDGWATWFATGVVFNDGDTMGQTITMQTRVRDVAGNEVMSDVTTFLYDPDAPSTV
                     LTFPVNGRAYREKITTISGNTLESPENNAGIDKIRIGLQRDDGIWKVAAGTSSTRSDP
                     NLLAGQIQGNNWTYPVGDSMFIDPVTSLPLQTATYTIHAWSYDLVKKPDDSYTNTESS
                     MTTDGAWIYDVQAPSSTIVLPVDETWYSDQPGYSLPTLSGTAVDNPGVNGAGLQKVQI
                     EVRDEDANPKVYWDEGLNDWFETAKWNDAGLSNGTFTYVTADLRNELTSGRTYRVRVR
                     TYDMAYNRLDATPTGNVESPEDITLLYQQENGDRINVNFFKWDSSAPVSAVVYPAHNQ
                     SLTDIDEISGTATDPGTGTGVYKVYVAVYQGSGGSTYLTSLAGGTFTGGLTWFEASTN
                     TASPVGWTLDTSGVSFPTGADYHVIVKSSDVVRNVETVDLAPGAAAAGTNHTKFVVPS
                     PQPDSDFTRPDTTLGEPHYTPSALATISGTALYATTEQIRLTQVTDTPNQFFDSNGVY
                     NGTPRIWVSTPLVSAENRWYGVDALSSEPIIWTDSMTVSNWAAGTKRYEIEARARYNL
                     IEEEDYNALPATRTFVVDGNDPTLTFIEPNFNYERSLPTISGTATDTAPGKFDQSAGA
                     FPVQVAISSAANAWWNWSSNWASASPIFKNATASDGSFNSANEPWYVASDMPPLEDGK
                     TYTVRVRIVDKAGRQPADFNQVFTVDISSPTAGIVLPVGGLPGIRTLPTLSGTATDNF
                     SDSINDVQVALRRHYADYRWLSEATTNFTVLQSTPYFISLKNHPELGYLSPDATFYMF
                     TPPNIDSNYTSGAEHVLVVRAMDKAGNSQVVFNSPLSSHTFRFDTNVPEPSVTFPLDD
                     GTPGQGRYRPEAVGDFPTSSLAGESPDKPDAVPPLSNAGLRQVQIKLSYLLAGDSWYW
                     LGSNFSSGTAAELAAWKFADSASPWAYNTDIVWPAWDQEYKLEARAEDLTTEGVVVGV
                     GNISPPDSVNFIVDRTSPTVSVTGPTGGVLPSLTVITGTANALLSGFSRVELKISTGT
                     GSLYYWTNGSTWTATETWLTSESTNTLIKDGETGWHYDVPGAMLVNNTTYTVVARARD
                     WAGNVTAGANYSTRTFAYDAQPPTVSIAHPVNNSTYSAVAVYVSTPVTGSAADVFLST
                     VAVAWTDLTEGTNKYFNGAIWTTGGPYYLGVTNGLTSWQFNDGDLTFANDHAYQVEAR
                     ATDLAGNNTYAGSKVIAYDVTKPTSTVTAPAGSYFASLGSIGGTASDDPGDAYLYEAR
                     LGTHTVGMAVKQEWSGGSGWWNGSAFSGGAPLYEEVTNTTTPPTSNSWTYSVPAGMQN
                     LLQSGATYWFVPRAVDRAGNAEFRDTAEGDIPSGTGRRSVYDSVKPTATVVVPVAGGE
                     IRSLPTVSGTAVDDYKMKNVRVAIRKNDPVTPLWYGADNNFSVAGTNPNFLQTTTNVV
                     TQTQMGWVFAPALMDDDFVGDVSYTIVVEATDDAGASQTTFSVPESSRTFLIDKLAPE
                     SAITLPAHDGDGQSGRYPPGNMGQTSTRFGGSSADQPTLNAGVEQVQILLSYLQDPDT
                     WYWTGTGFSSGAAESTYWRLASGTGTWVYTENVITWPAGDREYTLKVRGEDEATLGAG
                     SALDAGNVEVPSTEGTDIRRFIIDGSSPTVGITTPTAAVIRSLTNITGTASAALSGLN
                     MLQLKISTGSGVNMRYWTAGSTWTANEYWLATSTNVPGTQGVYGVPPSMLVNHTTYTL
                     VMRGLDWAGNLSAVYSTHTFLFDGSSPTASISFPLMNVAYSTYTLALSTPIQGSASDV
                     EAGLSTVTVAVQNVEDTTEFNGAGFVSNGGVPLYLPAQGTPASWTAADPDLYVNLEND
                     KRYDIYVRAFDGAGNQYTSANRRFRFDVEAPTAPINAPSAGYATGLTVVTGTVCDEWL
                     AVGNRNYEAGVGTHTVYIAFKKGGASWWDAGVPDFLSSDPVWYETVTDTNTDVDPGVK
                     VAYRWTYSVSGDLLTKLGPGGQMQIVTRPLDLAGNAPFGAGDAQADGKGITVLFDDKP
                     PVAGIELPAPNVNYRVATLPTLSGTANDTLTGNTGLQIVRVYLKNVDDVTYWQPSDNS
                     FASEILASTATLYQSSWTIVPPLANMRSNATYRLWVEARDGAGNWLSVPQSSFTSNAG
                     GVPFFFDKRVPFTQLVLPSSGSAINVPLTTLSGTAVDDPTGIGSGVVISGGNTIRLKI
                     IRSDGLYWMGNAFDVDVATRYPSALPLLSGTTVWGEKISPNEASFYGMGYKYTLQMRS
                     VDLAGNVEAPDPEGWFIYDVQLPTATITTPVTGSFLQALASIQGTADDRSTLASPRDF
                     EAGLVSTGVKVAAQRKTDGYWWDANNGTPPDRFVSPPGTPVWNDASFVGQASGTWDFS
                     FPSGALVNGASYYVQVKAVDLATNEQAVLSKSTFTVDTTSPSSRVMYPEATTYNSVAT
                     ISGTATDTLPGELDVVEVAIMQLTAPQERWNGSAWVGGGTPDIWLSSGVVNLVRQATW
                     TYVLPGTIWNNKNNKDFVVRARARDKAGNRATDVGSDIQFTITTPEAIPTIENPLPDH
                     QYRTITPNNFPVASGKGVNLRLSNGVNYRLVRESDKKQWDESSQTWIASATVPAEEYY
                     NAPIDPSGALLDWSTSTVNVSWTNNSSYTFIARGYNSAGEATVTPPSVFFYMDDDAPT
                     AATILPVNPPGAYNSMETLTGTAADVTSLMNDSMDEMQMRIKRGGEYWKGGSTWDTNS
                     AGSDFGVPFLPAGGKTVQWSTNPAVNWEDGRSYTLVLRPKDKATNQPLETITQAFVFD
                     ASTPTASLSTPTANAIYKNLPTLSGTAYDPIPAGPPYNNISDVSQVEIRIYWPNYNVY
                     WNHAEGDFTFGPGDAENAWTPAAGVAPWTYTHAQLASALSSKGDNYNAFNVSVRAWDA
                     AGNKQVIYSAPLSSRTFQMDTQPPLTTITKPAQDAGEGSAVGKYRPADLAGGDAFKGD
                     TADLPAGAASGVASQEVNLAAFVGNDTWYWLPTNMWSSGTVTETDAWRSASFYTSSFT
                     FLFQPDTNWLWDGAYQLKARARDKALNPTGTVGTQETPTVSTSSNHRRFIVDRTAPVS
                     RVTFPNSAYRTTLPLISGTVNADVSGLGTNAVRVRLWRANGDYWNGSAWQSGVVFATP
                     TYTVGNGTKTWTLPAGGYNMPVGLNLEDGTVHYISVDAVDLAGNFESGATIQFTVDNT
                     VGVLATTKPVNTDYYGGAGVLPDFPAKGLGDICGTASDTGSSGVALVEVQIEDSGWFW
                     DGNGFDTLESTYVPAVGSGVGLVNWTYPGGQGGQAIPNWASGKQYVIRTHYTDAAGNT
                     SADVTNAFIWDVAIPTATLADPNAALESSLSMLSGTAADTDEPFDSKLKKVQVAIEQE
                     PGTNMYWDGDSFESGERWLDAAGYVAGDLSVNPWTFTGTTPTWATDGTQYRVRVRAVD
                     RAHNVSQNLFISSCAFRFDNLAPTLTMTSPQAGQRHSILPTISGASSDTGPAGVAGVQ
                     VRVRYNDTVQFWVPGTDYSAGAYTQPDGELAWFAARTTNSWTNWFSTFTFFTDASFRV
                     EARAYDGAGNVSVVYATNTFIKDVDVPASGVTFPANGSIISDISMGVSGTAGENLALV
                     HEGQVQTVYMGVRDLHDADGSPWWNGTSGFSLADPVTINETSVTFSGSSSGTWNRLLS
                     GVSLESGVSYYITVRVQDNAVPANDEQFYSVRGSTFTFDATGPVVVITTPTLTVPQYV
                     NTLLTISGTADDVGLLSPVKAVQIAVSSGPWGVSGLWYNGAGGWGASPQWINASTGTP
                     SGNQWTYTFPNNNHWGQESLFEVRARGWDVPGTTGAFSATHQFIFDLKAPTATLVSPA
                     DEEYLSNDGTVQLSGTAVDSPAGIKSVEVAVSSSTVGGWWTGGDFVSPTEIYRTTASY
                     TPGSPDQWTWTEALWVTKLADGVTYYVKVRVTDKAGNQRVMTQTSFHYDASDPQAGVQ
                     EPDAAYERALPTLSGTATDTLPGRVTNVQLTISTGSGLSERYWTGSGWTADSTAWPLT
                     AAAVTPPYDSASENWIFTGSTPTWEYGKTYRIRVWATDNAGNKPDFALLGVKSFIFDD
                     QGPSLTMTSPQAGQRHSILPTISGASSDTGPAGVAGVQVRVRYNDTVQFWVPGTDYSA
                     GAYTQPDGELAWFAARTTNSWTNWFSTFTFFTDASFRVEARAYDGAGNVSVVYATNTF
                     IKDVDVPASGVTFPANGSIISDISMGVSGTAGENLALVHEGQVQTVYMGVRDLHDADG
                     SPWWNGTSGFSLADPVTINETSVTFSGSSSGTWNRLLSGVSLESGVSYYITVRVQDNA
                     VPANDEQFYSVRGSTFTFDATGPVVVITTPTAASPQYANSMPVISGTADDVGLLSPVK
                     AVQIAVSSGNFATATRWYDGAGGWETRAAGDPKWLDASTGTPSGNRWIYNFGAYVPWT
                     QAETYQARARGWDVPGTTGAWATAHQFTWDVWNPTATVYNPSNGGYMQASLDTISGTA
                     TDSPAGILKVEVAISSSATGAGASWWNGSSFSSASEIFYSTTNYNALPEPDQWEWARP
                     PDINMTNGNYYLVRLRVTDRAGNVRTQNAGDEGTTFIYDAEYPNSGYQRPSPGFVGGV
                     LSSWQALPTLSGTATDTAPGKVNLVQVAITTDSGNSYWNGSAWGGEYWHNAVARDGNG
                     FNSASEPWLLTGSTPVWQHNVAYRLKVRALDNAGNLQEGPSLDFTYDDQVAISTVNFP
                     ANDVYYQSLATLSGTMEDIPSDISAVQVALVNPLGNFWDQDTNNFPDDTVRWLPATVY
                     DSSWTYVDADLLGVLNSNVAYRVHVRGLDAAGNVPANPADFKVTGRPFHVDFSSPVSK
                     VSSLSTAATTYLKGGIDSIAGTADDSTGGSGVNEVKFRIRRVSSVGSDYRDPGSSSWN
                     NGVDAVNFPFTANDSMPLTNWSEDADIDAALFEDGYLYEIQSRAKDTAVPANVEVAYA
                     TAAFVVDKSTPVVGIVAPSNNSYHNTSSLPTISGTAADVVTGGGVPSGIQKVEVEVYN
                     MDGTVWWNANAGTWGGEVSTQAVALAVYPSSVTWAFYTSTPTLTDTTSYMVRVKAYDK
                     SDNQTPNFVTWQSSFTADFQAPQSEVLEPPLPDNGAVVYLPTISGTAADVGPADISHV
                     QVMIQRATGGNPYHTNPSPYNDGAFWEQYITSGSWKLNGGVPIWNTVPSYNPLNDRWV
                     FDMMTSGLYSAVAGGIRYYLEVRGVDKANNTEVVVSTRSFVFEPPAADSFISLPADNK
                     AYNQLNLIQGTGVNLEQITVQIQNMKDNSYWNDADGNSAGVGSFGGEAWITLTPGSPF
                     NYDNGSNINNIWVNGTSYTVRSKGYNVSIEEPVPDAKYIIFDTSEPVSGFRYPNAAFV
                     NNLLTISGTCADPPPSGGVSAGVLKTEVAVQRYGDTYWWDGSTWTAPGGTPGTWNTHT
                     TLSPSATFWTYVIAYSTHVQDGQIYKVRTRATDKTLASGLIEGLVDSEGNTVGEMTVV
                     YDVSMPTATITSVTNGQKRSSVAVASGTFSDPTPNHPTQGAEPVTVSVAVKDNQFGRY
                     WDGDSFENTSGVPTYLSATVYASSWAYTSMPTPGSYNDGIGVTIFVAAQDAAGNQNNL
                     FVSGVSSVTFVVDKAGPNAAFTTPATGNKWKADDLWILQGTAADPLVAPSYNPAGLND
                     RTQVQVQLYYLEAATTYYWNGSSFTALPVFTSESGWFSADNYYPDTASSGTWDYSASG
                     DLKNKWVSDKSYTFNVRARDLALPTGNLGATGGVYNVVVDTTPPVSAITSPTGDPLRS
                     LVTLSGTANGNLSGLSNVVFTLERNFPASAYFNGVGGWGAATDLPTQPNNFGAAQGDQ
                     IWTSTVVKTSDLADGTTYTVRVYATDVAQNGGNRQVTPALYSFLFDNAPPTVGLTTPS
                     AGNYGGPAAPLTVLSGTAVDLHSGIQKLEVKIYDETDERFWDGAAFNTTFSSFVAVNP
                     PYAAWQFSGITWPANKRYQIQARVTDRAGAVVETSTTTLIYDTTAPVAGITVSSNAYH
                     TPAQVATLYGTSNDAAANPAPGSQLDRIQISLLQDPLDASPAVDTLWWNGSAFGGVSA
                     DWRVVNSTLSNGNWNYPPSTPPGDGALTLLEGVKYRMQVRATDRATNVSSVVTSTFVV
                     DNVAPANFITVPLDEAGGDYESYLPTLSGTATDSAGTGPEPGRIANVQIRLRHQSGAY
                     WNQGLFPPDWDLGLPAASAWFNAIPNDGAFDGNSEPWHFSGSTPAWASGDTYNIHSRT
                     FDAAGQVSAISYSTFTYDDNKPQSVVDNPAANTVIRTLGSGVAGTAWDTGSPIQGVEV
                     AMRKLGGTTASGDPNVDVVGAWWNPIGDAFQTAGADPVFRAANFPGPNAWNYVGMGFD
                     DLDLESGASYYITTRSSDVAKNVETDFMTGSSTFTFDNTGPRSQFTQPALGAVMSGGE
                     AYYGALSTLSGTADDFKLPLGAANLNAGISTTTIKVAILDEDAAGGAKYWEETTPGFT
                     TGGSEVWLTAKSYLGRSSGTWTYTHSQLQANLINGHRYVVKSSATDLVSNLQPAIAST
                     TFVYDSSGPITVIATPPNGVNRTALTEITGTADDQPRLSLHPSLDRVGLDRVELQIID
                     LTAVPDLYWSGTSFITDTSTKVVLAVNGVDVQNWTYDNPDDMLTSGHRYRIQATAYDD
                     LNNALSPAVSEFIYDSSAPLSSVTRPAELVGYSLANPLPTISATILDQPTGGLLENAG
                     VKVDGVYTTIRQDSNKNDVPDGTDKYWDGDGFDTTSEVVQPPMTWQSGDLWISTSPTW
                     VSGFKYFIEVWAQDNLLNAESPRKLRTFTIDLAAPASRMSYPSAITFQNIVSISGTAQ
                     DDVSMITGTEYRLWHLGDNDVVGGSGGGTDQYYNGVSGVFGTSEKWNPATLGPVYITS
                     AVWTLAQEPGTPTYAGAHPEE"
     gene            complement(24455..24958)
                     /locus_tag="P5079_01220"
     CDS             complement(24455..24958)
                     /locus_tag="P5079_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28637.1"
                     /translation="MKQVWHVLMLAAVLTAGVRWGTGASPVSVDLRLSPAALEFDQGL
                     DVRDAEGRRVAAPAARDQLRRLFVGGLYALLMRPDLPRVAEVLSVLEKMWNVSSGTVG
                     HAVAAVWMACLDLWLPPVKRLNVTPFLCLLATLAVLLTQAAFGFFRTIPKTDSFLKSL
                     SSIVLRR"
     regulatory      complement(25091..25228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            25526..26536
                     /locus_tag="P5079_01225"
     CDS             25526..26536
                     /locus_tag="P5079_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28638.1"
                     /translation="MRPLNPFRTPPAKTPPDPRSEERFRRILWLHRLIILVLLAALGY
                     LRAPALMEWDRENFDLLRLSRVNQKAPHYKEIKKIIENLRYSGLPRLLRPDVRLELNF
                     EKKMWRLANIHRYTDDGEILLEDGKVGLCGQLATHTYFRIRHLFDHRYSIRFGRVSET
                     NFFTAPEASHIVLQITDLSNPQQPAFYILDPSFRRYGPIEEFGDYNFLEDMGLLAFLT
                     KRPPHETKPVNVSTPILITKNKLLLFHAATRNGRFDKDQFSLMVTALQRHQYRPEPLM
                     TLRVENGAPALVENEGLAGELLTTKKRAALRDMLWRFYRHVEEQTGGKAAVGAGKPTS
                     AK"
     gene            complement(26539..27414)
                     /locus_tag="P5079_01230"
     CDS             complement(26539..27414)
                     /locus_tag="P5079_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28639.1"
                     /translation="MKRPYGTVFLALLPLAVLAMGKEPVPSGHALLARCVEASGGGEA
                     YRQRRSVSYRFQEKSSHEGLRVVVDGRQYFKRPTGEDLWGREETTSPLGRTLTVFGPG
                     GAGVWDNAVAVKDPGLRDWAQAHLRENAFWFFLPHHVMESGSRVEEDGTGPFPGESKL
                     CRKLRVRLSASAWGGDIEELSLYLDTATPTLEGAVFRRAGEDYRLRYLYRKDFFFPEW
                     EIRDAEGRKILSFLAYDLDVSTGGADDLFDPSRAPSGGFFRRDELPTEGDEEDPDCKC
                     KRGGLFDLRRVRSPE"
     gene            complement(27411..28223)
                     /locus_tag="P5079_01235"
     CDS             complement(27411..28223)
                     /locus_tag="P5079_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28640.1"
                     /translation="MKSGRFLKVGLAAGAVFLLAAKKPPSGADYLEKSVQRAGGFSKY
                     QRVESIAYDFAERRFVDGKSVLSTGRHRFKLHDPAGLRGREELSSPSGRTVTVFTSSG
                     TWFWKDGRAVTEPAAVAAARGELERKIFWILAPFSLKEAACPVEYLGMGYVEGRLLRR
                     LEARKGAGLFPSEESFVLLVDSGTFRMEGATYRPPAAAPPQTLLFSDYAEVRSLVFPF
                     RRDVFDEDGRPLAVFTLLNLDVNTYLDENLFTSDFPEEVQSFPEKINTPVHP"
     gene            complement(28653..31259)
                     /gene="clpB"
                     /locus_tag="P5079_01240"
     CDS             complement(28653..31259)
                     /gene="clpB"
                     /locus_tag="P5079_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRY28641.1"
                     /translation="MDKLTIKAQEVLRSAQRLAEKKHHPRLEAAHLLSACLEVPDNLV
                     PAIVEKCGVPAGRLQKDVVESLARLPEVRGNVQMSASSAFREMLARAEEEASALRDDY
                     VSTEHFLLALLAVDGDDVAALLKKAGLSREGVLKAMSELRGTHRVTDQNPEDKYRALE
                     KYGRDLTELARRGKLDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRVVSGDVPEGLKSKRVVALDLGALIAGAKYRGEFEDRLKAVLKEIVAADGRVVLFID
                     ELHTLVGAGAAEGAVDAANLLKPMLARGELRCVGATTLDEYRKHVEKDAALERRFQPV
                     VVGQPTVEQTIAILRGLKERYELHHGVRIKDGALVAAATLSHRYIADRFLPDKAIDLV
                     DEAASRLRMEMDSMPVELDEVERKSRQLEIERQALRKESDTAARERLRKLESELADLQ
                     KRSKTLKTQWEAEKAALGRIREIKERLEQVRLEEHAAERAGDLGKAAEFRYGRLPALQ
                     KELEAAQERLDGLQKDTRMLKEDVDEEDVAAVVSKWTGIPVSRMLEGETAKLLKMEEN
                     LHRRVVGQDEAVRAVADSIRRARSGLADPNKPMGSFLFLGPTGVGKTELCRALAEFLF
                     DDERAMVRLDMSEYMEKHSVARLIGAPPGYVGFEEGGQLTEAVRRRPYAVVLFDEIEK
                     AHPDVFNALLQVLDDGRLTDGQGRTVDFKNTVIVMTSNVGSQHLSGAGNDKEVNTQVT
                     LALRAHFRPEFLNRIDEIIVFRRLSESDIAEIVDIQLAAVRRRLSDKRIDLVPAERLK
                     ARLAKFGYDPDYGARPLKRLLQKWIVDPLAKKLLAGEFREGDAVEADIDKAGEIGFQK
                     VRGRVSSAGRGE"
BASE COUNT         5240 a        10325 c        10136 g         5832 t
ORIGIN      
        1 cctcctccgg atgcgcgccc gcataggtcg gggtgccggg ctcctgggcc agcgtccaga
       61 cggcggaggt gatgtacacc gggccgaggg tggccgggtt ccatttctcc gaggtgccga
      121 acacgccgga gacgccgtta tagtactggt cggtgccgcc gccggaaccg ccgaccacgt
      181 cgttgtcgcc caggtgccac aggcgatatt ccgtgccggt gatcatggac acgtcgtcct
      241 gggcggtgcc ggagatgctc acgatgttct ggaacgtgat cgccgacgga taggacatgc
      301 ggctggccgg ggcggccagg tcgatggtga aggtgcgcag cttgcgcggg gactcggcgt
      361 tgaggaggtt gtcctgcgcc cagacctcga tgaaatactt gaagccggac acccacgtgg
      421 gcgaggtgct gatccacagg tcgccgctct gccacgtcat gggcggctgg acgacctcgg
      481 aggtggtgtc gaacccgtcc ccgtcccagt atttgtcggt gccgtccgga acgtcgttct
      541 tgttgctgtc ctgccgaatg gtggtgtaca cgccgtcgac cttcacgccg gcgttctcca
      601 gcagaccgcc ggtgggctgg tccaaaatgg tggcggaaat ggtgggcagc gggttggcga
      661 gactgtatcc caccagctcc gcgggccgcg tgacgctgga aagcggcgcg gaggagtcgt
      721 agatgaattc cgacaccgcc ggcgacaacg cgttgttcag gtcgtcatac gccgtggcct
      781 ggatgcggta ccggtggccg ctggtgagca tgtcgtcggg gttgtcgtag gtccagttct
      841 gcacgtccac cccgttcacc gccagcacca ccttggtgct ggtgtcggtg atgaaggaag
      901 tgccgctcca gtacaggtcc ggcacggcgg tgaggtcgat gatctgcagt tccacccggt
      961 ccaaccccac ccggtcgagc gaggggtgga gcgacaaccg cggctgatcg tcggcggtgc
     1021 cggtgatctc cgtcaacgcc gtgcggttca cgccgttggg cggcgtggcg atgaccgtga
     1081 tgggtccgga ggagtcgtag acgaaggtgg tggaggcgat ggccggctgg aggttgctca
     1141 ccaggtccgt ggccgaggac ttcaccacgt accggtgacc gttgatcaaa ttggcctgca
     1201 attgggaatg ggtgtaggtc caggtgccgg aggagcggcc cagataactc ttggcggtga
     1261 gccacacctc ggacccgccg gtggtgaacc ccggggtggt ctcctcccaa tacttggcgc
     1321 cgccggcggc gtcctcgtcc aaaatggcca ccttgatggt ggtggtgctg atgcccgcgt
     1381 tcaggttggc cgcgcccaac ggcagcttga agtcgtccgc ggtgccggag agggtggaca
     1441 gcgcgccgta atacgcctcg ccgccggaca tcaccgcgcc cagcgccggc tgggtgaact
     1501 gcgaccgcgg gccggtgttg tcgaaggtga aggtggagga accggtcatg aaatcggtct
     1561 ccacgttctt ggccacgtcg ctggaccggg tggtgatgta ataggaggcg ccggattcca
     1621 ggtcgaggtc gtcgaaaccc atgccgacgt agttccaggc gttgggcccg gggaaattgg
     1681 cggcgcgaaa caccgggtcg gcgccggcgg tctggaaagc gtccccgatg gggttccacc
     1741 aggcgcccac aacgtccacg ttgggatcac cgctggcggt ggtgccgccc agtttgcgca
     1801 tggccacctc caccccctga atcgggctgc cggtgtccca ggcggtgccg gccacgccgc
     1861 tccccagcgt gcggatcacc gtgttggccg ccgggttgtc caccacggac tgcggtttgt
     1921 tgtcgtcgta ggtgaaggtg gaataggaga tggccgacac ctggcccgcg gcgtcgaagg
     1981 tgcgggaatg gatgttgtag gtgtcgccgg aggcccaggc gggcgtggag ccggagaaat
     2041 gccacggttc ggagttgccg tcgaaagcgc cgtcgttggg aatggcgttg aaccaggcgc
     2101 tcgccgccgg gaggcccagg tcccagtccg gcgggaacag cccctggttc cagtacgcgc
     2161 ccgactggtg ccggaggcgg atctgcacgt tggcgatgcg gccgggttcc ggcccggtgc
     2221 cggcggagtc ggtggcggtg ccggaaaggg tgggcagata actctcatag tcgccgccgg
     2281 cctcgtccag cggcacggtg atgaagttgg cgggcgccac gttgtccacc acgaaggtgg
     2341 aggtgaccac ggaactcacg ttggtggccc tgtcggtggc gcgcacctgc atgcggtatt
     2401 tcacgccctc cagcagggtc aacgcgccgt cgccgggagg cgtggacggc ggatagttcc
     2461 agtttccgtt gctcaacgtg ctgttgacca cgcgccagtc ggcggacacg ccgccgaacg
     2521 ccgagccgtt ccaccacaag gtgtccaccg ccggactggc gtccagagga tcctgcagga
     2581 gggatatctg gatgcgatcc aactgggaac cgggagcggg gttggccgcg gcgtcgttgg
     2641 aggtgccgta gagcgtggcc acctgggccg gcgtgtggta cgcgttgctg gacacggtga
     2701 tgccggccac cggcgcggtg gtgtcgtaga tgagcgtggt ggtgctggtc tccaccacgg
     2761 cgccggcgcg gtcggtgacg cgcgcctgga tctggtaacg cttgttggcc ggccacgtga
     2821 tgccggagaa ctgccacgcc gcgtagggcg ggttgaccgc aacaaaagag gagaaggtgg
     2881 tgttgaacgc cgcgccgtcc cagaaccgtt cgtcggtctc gtcgtagatc ttcacctcca
     2941 gtttctggat gccggaatgc aggtccaccg cggtgccgct cagcacggtg agcggggcgg
     3001 ccgggccgcc gtagttgccg gcggacggcg tggtgagacc cacggtgggc ggggcgttgt
     3061 cgaacaggaa actgtacaac gccggcgtca cctgccggtt gccgccgttc tgggccacgt
     3121 ccgtggcgta cacgcgcacg gtgtaggtgg tgccgtcggc gaggtcggag gtcttcacca
     3181 cggtggaggt ccatatttgg tccccctggg ccgcgccgaa attgttgggc tgcgtgggca
     3241 ggtccgtggc cgcgccccag ccgcccacgc cgttgaagta cgcgctcgcc ggaaagttcc
     3301 gctccagggt gaacaccacg ttgctcaacc ccgacaggtt gccgttggcc gtgccggaga
     3361 gggtgacgag ggacctcaac gggtcgccgg tgggcgaggt gatggccgaa acgggcgggg
     3421 tggtgtccac caccacgttg tacacgccgc cggtggcgcc caaattgccg gtggggaggg
     3481 ccaggtcccg ggcgcgcacg ttgaaggtat aggacttgtc gctcacccat ttgttcttga
     3541 ggtctccgga ggcgctgtaa tcccaggtgc cggacgacgc cgtgtccgga taatagttgt
     3601 cggcgctgaa ccagccgctc tccgaggtga acaccggcag ggccgtgaag gacgagccgt
     3661 tccaataata ggtggtggcg gcctccagat aatacaactg cacctgcacc tgggtgcggt
     3721 cgttgagtcc cgccggattg taggacgggg ccaccagcgg gtcggcggcg gtcccctgaa
     3781 ggatccacag gtcgtcggcc ttccacttgt tgccggtggc cggagtggtg aaggcggcgt
     3841 tgggtccggc cttgtccacg acgaaagtga cggaggagac gccggacacg aagaggttgt
     3901 tctggttgcc ggcggcgtcc tgggcggcga cgaaaatggt gaccccgatg ccgtcgttgt
     3961 aggaacccgg cgtgggcatg gaggtgtacg cccaggagga ggcgtacacc gtggcggaca
     4021 ggtacgtggg caccccgctg gtgttctcga acgaatcgcc gtcccagtac cgtccgaact
     4081 gattgtcctt cacggccacg gacaccgtca ccggttccgc gccttgcgtg ggatggttcg
     4141 gcgtgggatc ggagaaggtg ccggacgcca ccgccaccga ggaacgtttc tggccgttgg
     4201 tcacggaagt gatggtggcg gtgggcatgg acacgtcgta gaccaccgtc atctccccga
     4261 cggtgttgcc ctcgctgtcc accaagccct cgatgagtcc ggaggccagc gtcttgtccg
     4321 tggcgcgggt gcggaccttg tagatctgcc cgtcctgcac gtgggtggaa taggcgatga
     4381 cgtaggtcca gaacgtggcg ctgggggaga gcgtggtgtg ggtgttccat gtgccggggg
     4441 tcccgccggg cgcggtccag gtggaaccgt cccaccagta ggtgtcgccg taccgctgga
     4501 cggccacctc ggtcttcagc accccggcgg aaacgccgcc cgacggcggc gggtcggcgc
     4561 aggtgccgga aatggtcaga agattgttca cgaacgccgc gttgggatac cgaaagccgc
     4621 tgacgggttc ggaggtgtcg aaaatgatgt atttggcgtc gggcacgggc tcctcgatgc
     4681 tcacgttgta gcccttgctg cgcacggtgt aggaggtgcc gttcacccag atgttgttga
     4741 tgttggatcc gttgtcgtag ttgaacggcg agcccggcgt caaggtgatc cacgcctcgc
     4801 cgccgaaaga acccacgccc gcgctgttgc cgtcggcgtc gttccagtag gagttatcct
     4861 tcatgttctg gatctgcacc gtgatctgtt ccaggttcac gccggtgccc tggatgaggt
     4921 tcaactggtt ataggccttg ttgtccgcgg gcagggagat gaaggagtcc gcggccggcg
     4981 gctcgaacac gaaactccgc gtggagacca ccacctccgt gttgttggcc ttgtccacgc
     5041 cccgcacttc caaataatac cggatgccgc cggccaccgc cgagtagagg ccggaggtca
     5101 tcatatcgaa cacccagcgg tcgttgaggg gattatagct cggcaccgtg ttccaaatgg
     5161 gcacgccgcc gttgagtttc cagcttccgg aggtgatgta ctgttcccag aaggcgccgt
     5221 cgttgtaggg agacgggttg gtgtggtagg gattgccgcc ggtggcccgc tggatcatga
     5281 cttgaacatg gctgatgtcc gcggggccca cgtccgcggc ggtgccggag atggtgggca
     5341 gatacaccac cgcgccgttg tccggcaggg gcggctcgag aacctccgac tgcggcgctt
     5401 gaaaatccgc cgtgaaggaa ctctgccagg tgacgaagtt cggcgtctga ttgtcggatt
     5461 tatcgtacgc cttcacccgc accatatagg aggtggtgtc ggtgagggtg ggcgtgctgg
     5521 tgtagaaggc ccaggtgacg gacgacggat acaccgccag cgccaccgcc tgagtggaga
     5581 cttccccgcc ccaggtcccg gcgttggcgt tccaccacac ggtgccgtcc atgttgtaga
     5641 cctccacctc caccttctgg atgccggagg gcaccccgcc gccggtgacg acgtcggccg
     5701 cggtgccgga aatggtcggc agggaggagg tgttgtgata gctgttgttg gacggcgcca
     5761 cgatgcccac caccggagtg gacttgtcca ccacgaacgc cgcggtggcg taggcgacct
     5821 cgacgttggc ggggacggcg gtgtccttgg cgcgggactg gatctcatac aagtatccgt
     5881 cctcgaacag cgccgcgtcg atgtccgcgt cctccgacca gttggtcaac ggcatcgagt
     5941 cgttggcggt gaacgggaag ttgaccgcgt ccacgccgtt gttccaggag ctggaacccg
     6001 ggtcccggta atcactgccg accgacgaca cgcggcggat gcggaacttc acctcgttca
     6061 cgccggagcc gccggtactg tcgtccgcgg tgccggcgat ggaatcgatg ccgcctttca
     6121 aatacgtggt ggccgcggtg ctcaaagagg acaccttgga caccggggag ctgaaatcca
     6181 cgtggaacgg ccggccggtg accttgaaat ccgccgggtt cgccggcacg ttgccggcgg
     6241 cgtccagccc gcgcacgtgc actcgatacg ccacgttgga gttcaacacg ccgagcaggt
     6301 ccgcgtccac gtacgtccag gaggagtcat acaccgtggc cggcagccac cgcaccgtgt
     6361 cgtcggggaa gttgttggtg tcctggtccc agaaatttcc gagcgggttc accaaggcca
     6421 cctgcaccgc gctgatgtcc gacgggatgt cctccatggt tccgctgagg gtggccaggc
     6481 tctgatagta gacgtcgttg gccgggaagt tcacggtgga gatcgccacc tggtcgtcgt
     6541 aggtgaaatc cagcgacggg ccctcctgca ggttgccggc gttgtccagg gcgcgcacct
     6601 tcaggcgata ggccacgttg tgctgccaca ccggcgtgga accggtcaac agccacggct
     6661 cgctggcgga gttgaacccg ttgccgtccc gcgccaccgc gttgtgccag tattccccgc
     6721 cccaggcgga accgttccag taggagttgc ccgaatctgt ggtgatggcc acctgcacca
     6781 ggttcacttt tccgggcgcg gtgtcggtgg cggtgccgga gagggtcggc agggcctgcc
     6841 aggaagaaag caccccaccc acgaacccgg gcgagggccg ctgatacccg gagttgggat
     6901 actcggcgtc gtagatgaac gtggtgcctt catcgccggc gttctgcgtg cgcacgttgc
     6961 cggcacggtc cgtcacgcgc agacgcacca ggtaatagtt gccgttcgtc atgttgatgt
     7021 ccggcgggcg ggcccattcc cactgatccg gctcgggaag cgcgttgtag ttggtggtgg
     7081 aatagaatat ctcggaagcg gaagagaagg aactgccgtt ccaccaactg gccccggccc
     7141 cggtggcgga ggaggagatg gccacctcca ctttcaggat gcccgccggg ctgtcggtgg
     7201 cggttccgga gatggtgtcc agcgaggcct gcatgtaccc gccgttggac gggttgtaga
     7261 ccgtggcggt ggggttccac acgtcccagg tgaactggtg ggcggtcgcc caggcgccgg
     7321 tggtgccggg cacgtcccag ccgcgcgccc gcgcctgata ggtctccgcc tgggtccacg
     7381 gcacgtacgc cccgaaattg tagatccagc ggttgcccga cggcgtgccc gtggaggcgt
     7441 ccagccattt gggatcgccg gcggcccggg tctcccatcc gcccgcgccg tcataccacc
     7501 gcgtggccgt ggcgaagttg ccgctggaca ccgcgatctg caccgccttc accggactca
     7561 acagccccac gtcgtccgcc gtccccgaga tcaccggcat gctgttggcg tattgcggcg
     7621 aagccgccgt cggcgtggtg atcaccacca ccggccccgt cgcgtcgaac gtgaacgtgc
     7681 ttccccgcac gctgtagaac tgttcgtcgt tcgccgggac cgcgttgtcc tgcacccgca
     7741 ccgtgatgta gtagctcacc ccgctctcca ggctcacccc gctcaacagc cggttccacg
     7801 tcccgctcga cgacccgctg aacgtcacgc tcgtctcgtt gatcgtcacc ggatccgcca
     7861 ggctgaaccc gctcgtcccg ttccaccacg gcgacccgtc cgcgtcgtgc aggtccctca
     7921 cccccatata caccgtctgc acctgcccct cgtgcaccag cgccaggttc tcccccgccg
     7981 tcccgctcac ccccatgctg atgtccgaga tgatgctgcc gttggccggg aacgtcaccc
     8041 cgctggccgg cacgtccacg tccttgatga aggtgttggt ggcgtagacc acgctcacgt
     8101 tccccgcccc gtcgtacgcc cgcgcctcca cccggaagct ggcgtccgtg aagaacgtga
     8161 acgtcgagaa ccagttggtc cagctgttgg tggtgcgcgc cgcgaaccac gccagctcgc
     8221 cgtccggctg cgtgtaggcg ccggccgagt aatccgtgcc ggggacccag aactgcaccg
     8281 tgtcgttgta ccgcacccgc acctgcaccc cggccacccc ggccggcccc gtgtcgctgc
     8341 tggcgccgga gatggtcggc aggatcgaat gccgctggcc cgcctgcggc gaggtcatgg
     8401 tcaaactcgg tccctggtcg tcgaagatga agcttttcac gccgagcagc gcgaaatccg
     8461 gcttgttgcc ggcgttgtcc gtggcccaca cgcggatgcg gtaggtcttg ccgtactccc
     8521 acgtgggcgt ggagccggtg aagatccagt tctcgctggc gctgtcgtac ggcggggtca
     8581 ccgccgcggc ggtcaacggc cacgccgtgc tgtccgcggt ccagccgctc cccgtccaat
     8641 accgctccga taaacccgaa cccgtgctga tggtcaactg cacgttcgtc acccggcccg
     8701 gcagggtgtc cgtggcggtg ccggagaggg tcggcaaggc ccgctcatag gccgcgtccg
     8761 gctcctgcac ccccgcctgc gggtccgagg cgtcgtaatg gaaactggtc tgcgtcatca
     8821 cgcgctggtt gcccgccttg tccgtcacgc gcaccttcac gtaataggtg acgccgtcgg
     8881 ccagcttcgt cacccagagc gcctccgtcc aggtccattg gtcgggactc cccggcgtgt
     8941 aggaagccgt ggtccgatag atttcggtgg gagagacgaa gtctccgccg gtccaccagc
     9001 cgcccacggt gctggaggac accgccacct ccaccgattt gatgcccgcc ggactgtcca
     9061 ccgcggtgcc ggaaagctgc acggtgccgt cgttgctcag atactcctcg tccgccgggg
     9121 aaacgagggt ggcggtgggc gctttcagat cgaaaatgaa ctggtgcgtg gccgagaacg
     9181 ccccggtggt gccgggcacg tcccagccgc gcgcccgcac ctcgaacaag gattcctgcc
     9241 cccaatgatt gttgttgggg aaagtgtacg tccactggtt gcccgacggc gtgcccgtgg
     9301 aggcgttgat ccattgcggg ctcgcgcccc agccgccggc accgttgtac cacaacccgc
     9361 tgacacccca gggcccgctg gacaccgcga tctgcaccgc cttcaccggg ctcaacagcc
     9421 ccacgtcgtc cgccgtcccc gagatcgtca gcaacgtgtt cacatactgc ggcaccgtca
     9481 acgtcggcgt ggtgatcacc accaccggcc ccgtcgcgtc gaacgtgaac gtgcttcccc
     9541 gcacgctgta gaactgttcg tcgttcgccg ggaccgcgtt gtcctgcacc cgcaccgtga
     9601 tgtagtagct caccccgctc tccaggctca ccccgctcaa cagccggttc cacgtcccgc
     9661 tcgacgaccc gctgaacgtc acgctcgtct cgttgatcgt caccggatcc gccaggctga
     9721 acccgctcgt cccgttccac cacggcgacc cgtccgcgtc gtgcaggtcc ctcaccccca
     9781 tatacaccgt ctgcacctgc ccctcgtgca ccagcgccag gttctccccc gccgtcccgc
     9841 tcacccccat gctgatgtcc gagatgatgc tgccgttggc cgggaacgtc accccgctgg
     9901 ccggcacgtc cacgtccttg atgaaggtgt tggtggcgta gaccacgctc acgttccccg
     9961 ccccgtcgta cgcccgcgcc tccacccgga agctggcgtc cgtgaagaac gtgaacgtcg
    10021 agaaccagtt ggtccagctg ttggtggtgc gcgccgcgaa ccacgccagc tcgccgtccg
    10081 gctgcgtgta ggcgccggcc gagtaatccg tgccggggac ccagaactgc accgtgtcgt
    10141 tgtaccgcac ccgcacctgc accccggcca ccccggccgg ccccgtgtcg ctgctggcgc
    10201 cggagatggt cggcaggatc gaatgccgct ggcccgcctg cggcgaggtc atggtgagcg
    10261 tgggcgccag gttgtcgaac cggaacgcgc aggaggagat aaacaggttc tggctcacgt
    10321 tgtgggcgcg gtccaccgcc cgcacacgca cacggtactg ggtaccgtcc gtggcccagg
    10381 tgggcgtggt gccggtgaag gtccaaggat tgacggaaag gtcgcccgcg acatacccgg
    10441 cggcgtccag ccagcgctcg ccggattcga aggagtcccc gtcccaatac atgttcgtcc
    10501 ccggctcctg ttcgatggcc acctgcactt tcttgagttt ggagtcgaac ggctcgtcgg
    10561 tgtccgccgc ggtgcccgac agcatggaaa gactgctctc cagggcggcg ttggggtcgg
    10621 ccagcgtggc ggtggggatg gcgacgtccc aaataaaggc gttggtcacg tccgccgagg
    10681 tgtttccggc ggcgtcggtg tagtgcgtgc ggatgacgta ttgtttgccg gaagcccagt
    10741 tcggaatggc ctggccgccc tggccgccgg gataagtcca gttgacgagt cccaccccgg
    10801 atcccaccgc cggcacatag gtgctctcca gcgtgtcaaa cccgttgccg tcccagaacc
    10861 acccgctgtc ctcgatctga acctccacca gcgccacgcc ggaggagccg gtgtcgctgg
    10921 cggtgccgca gatgtcgccg agccctttgg ccgggaaatc cggcagcacg cccgcgccac
    10981 cgtaatagtc ggtgttcacg ggcttggtgg tcgccaacac gcccacggta ttatccacgg
    11041 tgaattgaat cgtcgccccc gactcaaaat ttccagccaa gtccacggca tcaacggaaa
    11101 tataatggac cgtgccgtcc tccaaattga gacctaccgg catgttgtag ccgccggccg
    11161 gcagggtcca ggtcttggtg ccgttcccga ccgtgtaagt cggcgtggcg aagaccaccc
    11221 cgctctgcca ggccgaaccg ttccaataat cgccgttggc ccgccacaat cggacgcgga
    11281 ccgcgttggt ccccaggccg gacacatcgg cgttcaccgt gccggagatg agcggcaggg
    11341 tggtacggta agcgctgttg gggaaggtca cccgcgatac cggcgcggtg cggtccacga
    11401 tgaaccggcg gtggttgctg gaggtggaga ccgtcggcgt ttcctgcgtc cccaccgtgc
    11461 ccgtggggtt cagggccttg tcgcgggcgc gggccttcaa ctgataggcg ccgtcccaca
    11521 gccagttggt gtccggctgg aagaggaacg tgaaagacga cgtgtagaag ctggcggaac
    11581 gccaggcgtc cgtctccgtc acggtgccgg aggaccacat gttggtgggc agccagtacc
    11641 aggtgtcgtt gccgacgaac gccgccaggt tcacctcctg cgacgccacc cccgacgccg
    11701 ccccggccgg caggtccgcg gtgtcgcctt tgaacgcgtc tccgcccgcc agatccgccg
    11761 gacggtattt accgacggcg ctgccctcgc ccgcgtcctg ggccggtttg gtgatggtgg
    11821 tgagcggcgg ctgggtgtcc atctggaacg tgcggctgga caacggcgcg ctgtagatca
    11881 cctgtttgtt gcccgccgcg tcccacgcgc gcaccgacac gttgaacgcg ttgtagttgt
    11941 ctcctttgga cgacagcgcc gaagccagtt gcgcgtgcgt gtaggtccac ggcgccaccc
    12001 cggcggcggg ggtccaggcg ttctcggcgt cgcccggacc gaacgtgaaa tcgccctcgg
    12061 cgtggttcca atacacgttg tagttgggcc agtagatgcg gatctccacc tgactcacgt
    12121 cggagatgtt gttgtacgga ggcccggcgg ggatggggtc ataggccgtg cccgacaggg
    12181 tcggcaggtt cttgtagatg gcgttggccg tgggcgtgga aagactggcc gtgggcgtgg
    12241 aggcgtcgaa gacgaacgcc tgggtgatgg tctccagcgg ctggttggtg gccttgtcct
    12301 tggggcgcag gaccagcgtg taggagcggc cgtcctccca gttcaccgcc gggttggtgg
    12361 accactgcac ggtcttgccg cccgccggca ggaaaggcac gccgaaatcc gacccggcgg
    12421 agttggtgtc ccaggtgctg cctcctttcc aatattcgcc gccgcgtttg atgcgcattt
    12481 gcatctcgtc catggaatcg ttcatgagcg aggtgacgtc ggccgcggtg ccggtcagtg
    12541 tctccatgga gttgtacgcc cccggcgggt tgaccggcaa aatggtggcg gcggtggggg
    12601 cgtcgtcgtc catgtagaag aagacggaag gcggcgtgac ggtcgcctcg ccggcgctgt
    12661 tgtacccgcg cgcgatgaag gtgtaggagg agttgttggt ccagctcacg ttcacggtgc
    12721 tggtgctcca gtccagcagg gcgcccgacg ggtctatcgg cgcgttgtaa tattcctcgg
    12781 ccggaacggt ggccgaggcg atccaggtct gcgacgactc gtcccactgt tttttgtccg
    12841 attcgcgcac caaccggtaa ttgacgccgt tggagagccg caggttgacg cctttcccgc
    12901 tggccacggg gaaattattc ggcgtaatgg tgcggtactg gtggtccggc agggggttct
    12961 cgatggtggg gatggcctcc ggcgtggtga tggtgaactg gatatccgag cccacatccg
    13021 tggcgcggtt ccccgccttg tcgcgggcgc gcgccctcac cacgaaatcc ttgttgttct
    13081 tgttgttcca gatggtcccg ggcagcacgt aggtccaggt ggcctgccgc accaagttca
    13141 ccacgccgga ggacagccag atgtccggcg tgccgccgcc cacccaggcg gaaccgttcc
    13201 aacgttcctg cggcgccgtg agctgcatga tcgccacctc caccacgtcc aactcgcccg
    13261 gcaacgtgtc ggtggcggtg ccggagatcg tcgccaccga gttgtaggtg gtggcctccg
    13321 ggtacatcac ccgggaggac ggcgaggtgg tgtccaccgt gaaggtggac ttggacaaca
    13381 ccgcctgctc gttggtggcc aagtccacgg ctttcacctg cacataatag gaggccccgt
    13441 tcaccagcgc cccggacggg aaggagaagt cccaggtgcc gctggcctgg ccgacaaaag
    13501 aggcgtcgtt ccacaccggc gtgccgggag gagacacgaa ccggtccggc ggggtaccgt
    13561 tgttggcgtc ccaccaatac ccgtcggtct ttctctgcgc cgccaccttc acgccggtgg
    13621 agacgaggcc cgcctcgaaa tcccgcggcg aggccagcgt gctccggtca tcggccgtcc
    13681 cctggatgga cgccagggcc tgcaggaagg agccggtgac cggggtggtg atggtggcgg
    13741 tgggcaactg cacgtcgtag atgaaccacc cttcggggtc cggcgcttcc acgttgccgg
    13801 cgaggtccac ggaccgcatt tgcagcgtgt atttgtagcc catgccataa aaactggcct
    13861 cgttggggct gatcttttcg ccccacaccg tcgttccgct caacagcggc agcgccgacg
    13921 gataccgcgt ggccacgtcc acgtcgaagg cgttgcccat ccagtacagg ccgtcggacc
    13981 ggatgatctt cagccggatg gtgttcccgc ccgaaatcac caccccggac ccgatcccgg
    14041 tggggtcgtc caccgcggtg ccggagaggg tggtcaacgg aacgttgatg gcgcttccgc
    14101 tggagggcag gaccagctgg gtgaagggaa cccgcttgtc gaagaagaac ggcacgccgc
    14161 cggcgttgga agtgaaggac gactggggca cggaaagcca gttcccggcg ccgtcgcgcg
    14221 cctccaccca cagccgatag gtggcgttgg agcgcatgtt ggcgagcggc gggacgatgg
    14281 tccaggagga ttgatacagc gtggcggtgg aagccaatat ctcggaggcg aaagaattgt
    14341 ccgacggctg ccagtaggtg acatcgtcca cgttcttgag gtagacccgc acgatctgca
    14401 gcccggtgtt gccggtcagg gtgtcgttgg cggtgccgga aagcgtcggc aacgtggcca
    14461 cccggtagtt gacgttgggc gcgggcagtt cgatgcccgc caccggcggt ttgtcgtcga
    14521 agagcaccgt gatgcccttg ccgtccgcct gggcgtcgcc ggcgccgaac ggggcgttgc
    14581 ccgccaggtc cagcggccgc gtcacgatct gcatctggcc gccgggaccg agtttcgtca
    14641 acaggtcgcc ggagaccgag taggtccagc ggtaggccac cttcacgccg gggtccacgt
    14701 cggtgttggt gtccgtgacg gtctcatacc acaccggatc gctgctcagg aaatccggca
    14761 cgccggcgtc ccaccaggag gcgccgccct ttttgaaagc gatgtacacg gtgtgggtgc
    14821 ccacgcccgc ctcgtagttc cggtttccga cggccagcca ttcgtcgcac accgtgccgg
    14881 tgacgaccgt caaccccgtc gcgtatccgg cggacggcgc gttgatgggc gcggtgggcg
    14941 cctccacgtc gaaacggaaa cgccggttcg ccgaggtgta ctggttgccc gccccatcga
    15001 acgcccgcac atagatgtcg taccgtttgt cgttctccag gttgacgtag aggtccgggt
    15061 ccgccgcggt ccagctggcc ggcgtgccct gcgccggcag gtagaggggc acgccgccgt
    15121 tgctcacgaa accggcgccg ttgaactccg tggtgtcttc cacgttctgc accgccaccg
    15181 tcacggtgga gagacccgcc tccacgtccg acgccgaccc ctggatgggc gtggacaacg
    15241 ccaacgtgta ggtggaatac gccacgttca tcagcgggaa ggaaatcgaa gccgtgggcg
    15301 aggatccgtc gaaaaggaag gtgtgggtgg agtagaccgc ggagaggttg ccggcccagt
    15361 ccaggccgcg catcaccagg gtgtaggtgg tgtggttcac cagcatcgac ggcggcacgc
    15421 cgtaaacgcc ctgggtcccg ggaacgttgg tggaggtggc cagccaatat tcgttggcgg
    15481 tccaggtgga ccccgccgtc caatagcgca tgttgacgcc ggaacccgtg ctgatcttga
    15541 gctggagcat gttcagtccg gacagggcgg ccgaggcggt gccggtgatg ttggtgagcg
    15601 aacggatgac cgccgcggtc ggcgtggtga tgcccaccgt cggcgaggag ccgtcgatga
    15661 tgaacctgcg gatgtcggtg ccctcggtcg acggcacctc cacgttcccc gcgtccaaag
    15721 cactccccgc ccccagcgtg gcctcgtcct ccccgcgcac cttcagcgtg tattcccggt
    15781 cccccgccgg ccaggtgatc acgttctccg tatacaccca cgtccccgtg ccgctcgcca
    15841 gacgccaata cgtggactcc gccgccccgc tggaaaagcc cgtccccgtc caataccacg
    15901 tgtccgggtc ctgcagatag ctcaacagga tctgcacctg ctccaccccc gcgttcaacg
    15961 tcggctggtc cgccgaactc ccgccgaacc gggtcgacgt ctgccccatg ttccccggcg
    16021 gataccgccc gctctgcccg tcgccgtcgt gcgccggcag cgtgatcgcg ctctccggcg
    16081 ccagcttgtc gatcaggaac gtccgcgacg actccggcac gctgaacgtc gtctgcgacg
    16141 cccccgcgtc gtccgtcgcc tccaccacga tcgtgtagct cacgtccccc acaaaatcgt
    16201 cgtccatcag cgccggcgcg aacacccacc ccatctgcgt ctgcgtcacc acgttcgtcg
    16261 tcgtctgcag gaagttcggg ttcgtccccg ccaccgaaaa gttgttgtcc gccccatacc
    16321 acaagggcgt taccggatcg ttcttccgga tcgccacccg cacgttcttc atcttgtaat
    16381 cgtccaccgc cgtcccgctc accgtcggca gcgaccggat ctccccgccc gccaccggca
    16441 ccaccaccgt cgccgtcggc ttcacgctgt catacaccga ccgccgcccc gtccccgacg
    16501 gaatatcccc ctccgccgtg tccctgaact ccgcgttccc cgcacggtcc accgccctcg
    16561 gcacgaacca atacgtcgcc ccgctctgca gcaggttctg catccccgcc ggcaccgaat
    16621 acgtccacga attcgacgtc ggcggcgtcg tcgtgttcgt cacctcctca tacaacggcg
    16681 ctccgcccga aaacgccgac ccgttccacc acccgctccc cccgctccac tcctgcttca
    16741 ccgccatccc caccgtgtgc gtccccaacc gcgcctcata cagatacgcg tcccccggat
    16801 cgtccgacgc cgtcccgccg atgctcccca gcgacgcgaa atacgacccc gccggagccg
    16861 tcaccgtgct cgtcggcttc gtcacgtcgt aggcgatcac cttcgaaccc gcatacgtgt
    16921 tgttccccgc caggtccgtc gcccgcgcct ccacctggta ggcgtggtcg ttggcgaacg
    16981 tcaggtcccc gtcgttgaac tgccagctcg tcagcccgtt cgtcaccccc aggtaatacg
    17041 gccctcccgt cgtccaaatc gccccgttga aatatttgtt cgtcccctcc gtcaggtccg
    17101 tccacgccac cgccaccgtg ctcaaaaaca cgtccgccgc cgaccccgtc accggcgtcg
    17161 acacatacac cgccaccgcc gaatacgtcg agttgttcac cgggtgcgcg atgctcaccg
    17221 tcggcggctg cgcgtcgtag gcgaacgtcc gcgtcgaata gttcgccccc gccgtcacgt
    17281 tccccgccca gtcccgcgcc cgcgccacca ccgtatacgt cgtgttgttc accagcatcg
    17341 ccccgggcac gtcgtagtgc caccccgtct ccccgtcctt gatcagcgtg ttcgtgctct
    17401 ccgacgtcag ccacgtctcc gtcgccgtcc acgtcgaccc gttcgtccag taatacaacg
    17461 accccgtccc cgtgctgatc ttcagctcca cccggctgaa tcccgacagc agcgcgttcg
    17521 ccgtccccgt gatcaccgtc aggctcggca gcaccccccc cgtcggcccc gtcacgctca
    17581 ccgtcggcga ggtccggtcc acgatgaaat tcacggaatc gggagggctg atgttcccca
    17641 cccccaccac cacgccttcg gtggtcaggt cttcggcccg cgcttccagc ttatactcct
    17701 gatcccacgc gggccacacg atgtccgtgt tgtaggccca cgggctcgcc gaatcggcga
    17761 atttccaggc cgccagttcc gccgcggtgc cgctggaaaa attcgacccc agccaatacc
    17821 acgaatcccc cgccagcaga tacgacagct tgatctgcac ctgccgcaac ccggcgttcg
    17881 agaggggggg caccgcgtcc ggcttgtccg gggactcgcc ggccaggctg ctggttggga
    17941 aatcgccgac ggcctccgga cggtagcggc cctggccggg cgtcccgtcg tccaggggga
    18001 acgtgacgct gggttcgggg acgttggtgt cgaaccggaa cgtgtgggag gacaacggcg
    18061 agttgaacac cacctggctg ttgccggctt tgtccatcgc ccgaaccacc agcacgtgct
    18121 cggccccgct ggtgtagttg ctgtcgatgt tgggcggggt gaacatgtag aacgtggcgt
    18181 ccggcgagag gtacccgagt tccggatggt tcttgagact gatgaagtag ggcgtgctct
    18241 gcagcaccgt gaagttggtg gtggcctcgc tcaaccagcg gtaatccgcg tagtgccggc
    18301 gcaaagccac ctgcacgtcg ttgatgctgt ccgaaaaatt gtccgtggcc gtgccggaaa
    18361 gggtcggcag ggtccggatg ccgggcagtc ctcccaccgg caggacgatg cccgccgtgg
    18421 gcgaggagat gtccacggtg aacacttggt tgaagtccgc cggctggcgg cccgccttat
    18481 ccacgatacg cacgcgcacg gtgtaggtct tgccgtcctc caggggcggc atgtccgacg
    18541 ccacatacca cggctcgttg gcggaattga acgagccgtc ggaagcggtg gcgttcttga
    18601 agatgggcga ggcggaggcc cagttgctcg accagttcca ccaggcgttg gccgcggagg
    18661 aaatggccac ctgcaccggg aaagcgccgg cggactggtc gaattttccg ggggcggtgt
    18721 cggtggcggt gccggagatg gtgggcaggg accgctcgta gttgaagttc ggttcgatga
    18781 aggtgagggt ggggtcgttg ccgtccacca caaaggtccg ggtggcgggc aaagcattgt
    18841 aatcttcttc ctctataagg ttgtaccgcg cccgcgcctc gatctcatac cgtttcgtgc
    18901 cggccgccca gttcgacacc gtcatgctgt ccgtccagat gatgggctcg gaactgaggg
    18961 cgtccacccc ataccaccgg ttctccgcgg acacgagcgg ggtagaaacc cagatccgcg
    19021 gcgtgccgtt gtagaccccg ttggagtcga agaactgatt cggggtgtcg gtgacctgcg
    19081 tcaaccggat ctgttcggtg gtggcgtaaa gcgccgttcc gctgatggtg gccagggccg
    19141 acggggtgta atggggctct cccagggtgg tgtccggccg ggtgaagtcc gaatccggct
    19201 ggggcgaggg caccacgaac ttggtgtggt tggtgccggc ggccgcggcg ccgggagcca
    19261 gatcgaccgt ctccacattc cggacgacgt ccgaggattt gacgatcacg tggtagtcgg
    19321 cgccggtagg gaacgacacc cccgaggtgt ccagcgtcca tcccacggga gaagccgtgt
    19381 tggtgctggc ttcgaaccag gtgagccctc cggtgaacgt cccccccgcc agactcgtca
    19441 aataggtgct tcccccgctg ccctggtaga cggccacgta gactttatac accccggtgc
    19501 cggtgccggg atcggtggcc gtgcccgaga tctcatcgat gtcggtcaac gattggttgt
    19561 gcgcggggta gacgacggcg ctgacggggg cggaggagtc ccatttgaag aagttcacgt
    19621 tgatccggtc cccgttctcc tgctgataca acagcgtgat gtcctccggc gattcgacgt
    19681 tgcccgtcgg cgtggcatcg agccggttgt aggccatgtc gtaggtgcgc acgcgcacgc
    19741 ggtaggtccg gccgctggtg agttcattcc ggaggtccgc ggtgacgtag gtgaaggtgc
    19801 cgttggagag ccccgcgtcg ttccacttgg ccgtttcgaa ccagtcgttg agcccctcgt
    19861 cccaatagac cttggggttg gcgtcctcgt cccggacctc gatctgcacc ttctgcaggc
    19921 cggcgccgtt gacgccgggg ttgtccaccg cggtgcccga cagggtgggc agggaataac
    19981 cgggctgatc ggagtaccac gtctcgtcca cgggaagcac gatggtgctc gacggcgcct
    20041 gcacgtcgta gatccaggcg ccgtccgtcg tcatggaaga ctccgtgttc gtgtaagagt
    20101 cgtccggctt tttcaccagg tcgtaggacc aggcgtggat cgtgtaggtg gcggtctgga
    20161 ggggcaacga ggtcacgggg tcgatgaaca tgctgtcccc caccgggtaa gtccagttgt
    20221 tcccctgaat ttgcccggcc aggaggttgg ggtcggaccg cgtggaactc gtcccggccg
    20281 ccactttcca gatcccgtcg tcccgctgga gcccgatccg gatcttgtcg atgccggcgt
    20341 tgttttcggg gctttccagc gtgttgccgc tgatggtggt gatcttttcc cggtaggcgc
    20401 ggccgttcac cgggaacgtg agaacggtgg aaggggcgtc gggatcatat aggaaagtcg
    20461 tcacatcgga catgacctcg ttgccggcca cgtccctcac ccgggtctgc atggtgatgg
    20521 tctgtcccat ggtgtcgccg tcgttgaaga cgacgcccgt ggcgaaccac gtggcccagc
    20581 cgtcggtggt gctggccggg ttccacaggt tcgtcgcggt gacccagtcg cccacgccgg
    20641 acagggccga ctcgtcccaa tacttatcgg ccccttcgca atagagctgg tattccacca
    20701 ggctgacccc gctgtccacg ggactgccct gggcgaaatc gctggccgtc cccgaaaggg
    20761 tggccaagga cccccactgg gaattttccg aaatgagccg gccgtaggcg ttgggggcgt
    20821 cgccggtgga gggatagatg atctcgatgg acgggttgta ggtatcgaaa cggaaccggt
    20881 gcttatgggc atccgacggc gtcggcggat tgggcggctc ttccgaagga agttcccggt
    20941 tggcgccgtc gtaggcgcgg gcccatattt cgtactgccc ccggtcctcg aagaaggagt
    21001 ttttcagaaa cttccagctt ccgttgctcg gcgtgacgga acttcccgac accgtgtgcc
    21061 aatacggcgt ggagttccac gtgtagttgg actgcagcca actgttgtcg ttggtcacgc
    21121 cgatgctgga ccggtggacg gccaactcca ccttggcgaa cccgtccagc ggcagaccca
    21181 gattgtcggc catggtgccg gagatgtacg gcagggaggc gtagttgaaa tagctggtgt
    21241 tggtggaggg cgtcaccagg gtgctggtgg gggcgtgcac gtcccatttg aactcgcgga
    21301 ccgtgctggt ggaggtgtgc cccagcatgt ccgtcgccgc gaacttcatc tggtaggtct
    21361 gtccttccgt ccaaaaattg ttgccgccgg gcacgttcca ataccaggaa cagtccgggt
    21421 cgccccgagg cggattcccg gccggcgcgg aaatccctcc cgtgaagatc acgtagttgg
    21481 tggtgtgggg ggaccagtgc cccgccagac catcccagtt gtagtagtcc gtcccatgga
    21541 tcacgtaaag tttggaagta gcgctgttca cgcccgaggt cacgtcctgc accacgcctt
    21601 ccacggtggc caggtccccc atgcggctca cggcccccgg cgacgtcaat tcgttgttga
    21661 tggggttgga ccggtccaac gtcacgtggg cctccagaaa cggggtggga ccgcggccga
    21721 aatcgtcgga caccgtccgg actttgatgg tgtaggagtt gctgtccacc tgcacgcccg
    21781 tccgccagcc gtcgttcccc gtgccgccgt gcccctcgaa catccactgc caggtgctcg
    21841 gcgcgctcag ccccgtgctg atccatatct cggtgtcgct cgtgaaactt ccgccggcgc
    21901 cggacagata gttcccgctg ttgtcgttgc ggatggagat ctccgccccc gtctgaatcc
    21961 cccccgcgcc gtccccccgc atcatgtccg ttttccccgc caggtaatcc acccattccg
    22021 cgtaactcgt ggcggccgtg ggcagggtga gggtccccgt gggccgggtg acggtgaagg
    22081 tggagttctg ctggacggac tggccgtcgg ggttcaccac agacaggtgc cacaacccca
    22141 gcgggaggtt ggccccgatg gcgatggtgg cggtgaacga actgacgccg tgcatggtga
    22201 tgctggacac gttgatccct ggcccgttgt tgttgatggt gatggacgac ttctgtagtt
    22261 cgctccaata ctggtagttg ccgccctgga cgagcaggtg ccggtaaggg atgacgcccg
    22321 gttccgtccc ggcattttca ccaccggaga ggtccccccc gttgtcgtcg gtgatggagg
    22381 tgatggacgg ccccgggatg ttgtaggccg tgacggcctc ggcgctcttg ccgtcggggt
    22441 tggtcacccg gatgtcgtaa ggtccgtcgt ccacgggggc caccagttca agatgcgcca
    22501 cgatggaggt ggagtacacg tcgtaggtca tggtgaaatc gttggtcact ccccccaagg
    22561 agccgatgaa ctccaacacg ggactctttg tgggcagttt gaccagcccc gagatgttgg
    22621 tgagttgcag gtcgtaggtc ttggtggcgt aaggagcgtt ggtggggctg gtggcgatgg
    22681 acatactgct caaatccgga taggggtagt tggaaatgag gatccggtcg aacgtcaccc
    22741 cgtcgtcgcc gacgaacacg aagggaagcg ggttggggta atccacgtac tgacaaaggt
    22801 tgggccagga ggagttcccg ctttgaatca tggtggaggc cggagctgcc caatcggcgg
    22861 ccaacacgga ggtggacccg gtgatgtagt aaagctgtcc gccagcgccg gccaggtttt
    22921 ccacgaacat caccaccacg gtgtccgcca cctgggggca ggtagaacgg gaaataccga
    22981 tggtgcagtt ctggtaatcg ccgccgccgc tgatggggtt gaaatcgaag ttagtggaca
    23041 ggctgttgtt gttcagaatg cgggcgtagg acagcgtccc caccgtggta ccgcccacat
    23101 cgttgatgga aatgacgtgg aggttccccg ccgagtccac cgccatggac acgttcttgg
    23161 tgtccctgaa ccagttgact cccgaatcat ccatccggtc gttgtaaagg ttcgtgacgt
    23221 tgttgtcgta atccacctcg gtgctggggt tccgaacgct ccaataaccc tcagtgtcgt
    23281 tcgtgggcac atattccccg gccgcccaaa cgctgtcgta tccgggcctg ttcacaagga
    23341 cgtttttata accgagagag tcatctgcaa gggccggagc acagttggcc tcattctggg
    23401 acgaccaata atggacggtc ccaccccagc cgtccacgcg gccggggttg acggacttcg
    23461 cgaaaatggt gaccagatcg ttgcctccgg cgatcccggc cccgtcatcg ccgccgttgg
    23521 cggacaccca cacgtaggtc gcccggtcca ccgcgatgga caccggccac ccggcgtatt
    23581 gagcggggtc gttccccgtg caggccgtag gctggtaata cccccgagcc tgggaagccg
    23641 ttcccgtagg gtcccagttg atggttccgt tgctttccag gttccccatc cgcgtgaaaa
    23701 tggtccggtt cgcggggaga acggattggg aacatccggc cgccacatac acgcggtcct
    23761 ggggcgcgtg gtaccacacg ctcaccatgg agctgacgtt cacgggactg atgccggacc
    23821 aatcgaaaat cgtttgcggg atgctccagg aaccggcgtt gcccgttttg gaataggtcc
    23881 agcagggaac accggtggtc aaggtggtgc tggtgtaaaa aacccagtaa ccctcgtccg
    23941 tggccaccac ttttcgctgg ttggaggcca tggtggtgcc gataccgtcc gccactttct
    24001 ggggatcggc ctggacgggg accgccgccg acatccacaa aaacagcccc aaaagaaagc
    24061 cggtcgaaag aggggccgtc cggcccctcc acgcgcacgc gcgttgaaac gacgtcaaat
    24121 aataaaaaag agccgccacc ccctgggcac tcagcgctcg acactcagca ccaagaaccc
    24181 gagaaatggc tcggacggtc cggacgcacc tcgtcccttc cgcgcgcgcg ttgtatctaa
    24241 ggacttcgac cttttggctc acgtgtcctc ttcgtttttt ccgtttttac gcgccgcaca
    24301 cgtcgtctcg cttcgtttta tgtcgtttcg agcgcgtttc ttcccttgtt ccggacggga
    24361 gccttcggct ctggtggcat gttcccgtcg acgaacacac aacacccccc ggcgcagaaa
    24421 cgccgacaag gggggctaag gaagcccgga gggattaacg gcggaggacg atggaagaga
    24481 gggacttcag gaaggaatct gttttgggga tggttctgaa aaatccgaag gccgcctggg
    24541 tcagcaggac ggccagcgtg gccagcagac acaaaaaggg cgtcacgttc aagcgcttga
    24601 ccggaggcag ccaaagatcg agacacgcca tccacaccgc cgccacggcg tgccccaccg
    24661 tgccggaaga cacgttccac atcttttcca gcacggacag cacctcggcc acccgcggca
    24721 ggtccggccg catcaaaaga gcgtagagcc cccccacgaa caaccggcgg agttggtccc
    24781 gcgccgcggg cgcggccacg cgccgccctt ccgcgtcacg gacatccagc ccctgatcga
    24841 attccaacgc cgccggcgag agacgcaggt ccacggagac gggagacgcc ccggtgcccc
    24901 accgcacacc ggccgtcaac accgccgcca acatcaggac gtgccacacc tgcttcatta
    24961 tcgcgcctct ccaagtacaa aacaacaacg ccgagctttc cgtttccaag actccgaatc
    25021 tggaagaaac ggaaaacccg gcgttcagct gacagactga ctcaaccgag cttcctcgcg
    25081 ctttccactt ttcggcaacc tggcgatcct gcgtgcactc aggcaggacc catggctttg
    25141 ccatgtccgc tgaatcccag acatggtccc gccacccacc caggcgacgg gacgtcccac
    25201 gattgctcgt ggtttgccct tatcggagtt cgattccaat gatcagacga tgcttccgtt
    25261 acaagtctaa gccggaaccg gtttttgtca agggttttct caatgggact tttgtcaccg
    25321 atttccaagc cggcgccgtc gagggaaacg ggaaactctc gggcctcccg tcggattcat
    25381 ccacccccgc ccccggccgc cgccggcgtt aagcgtcttt acgccacgcc cggcagggat
    25441 cctccgcccc gatgagcgga acggtacccg acagaggaag gtttaatgat tttccggggg
    25501 acgatggtgt ataatctacc gcgccatgcg cccgctgaat ccgtttcgca ccccgccggc
    25561 caagacccct ccggacccac ggtccgagga gcgttttcgt cggatcctct ggctgcaccg
    25621 tctcatcatc ctcgtcctcc tggcggccct ggggtatctc cgagcgccgg cgctgatgga
    25681 atgggaccgg gaaaacttcg accttctgcg cctcagccgc gtcaaccaaa aagcccccca
    25741 ctacaaagaa atcaaaaaga tcatcgaaaa cctccgctat tcgggcctgc cgcggctcct
    25801 gcggcccgac gtgcggctgg aactcaactt cgagaaaaag atgtggcggc tcgccaacat
    25861 ccatcgctat accgacgacg gcgaaatcct cctggaggac ggcaaggtcg gcctctgcgg
    25921 ccaactggcg acgcacacct atttccggat ccgccacctg ttcgaccacc gctacagcat
    25981 ccgtttcggc cgggtctccg aaaccaattt ttttaccgcc cccgaagcct cccacatcgt
    26041 gctccaaatc accgaccttt ccaaccccca acaaccggcc ttctacatcc tggatccgtc
    26101 tttccgtcga tacggcccca tcgaggagtt cggcgattac aacttcctcg aggacatggg
    26161 gctcctcgcc ttcctgacaa aaaggccccc gcatgagacc aaaccggtca acgtctccac
    26221 gcccatcctg atcaccaaga acaagctgtt gctgttccac gcggccacgc ggaacggccg
    26281 gttcgacaag gaccaatttt ccctgatggt cacggccctc cagcgtcacc aataccggcc
    26341 ggaacctctg atgaccctgc gcgtggagaa cggggccccg gcgctggtgg aaaacgaagg
    26401 actggccggc gaacttttga cgacgaagaa acgcgcggcg ctgcgggaca tgttgtggcg
    26461 tttttacagg cacgtggagg aacaaaccgg cggcaaagcc gccgttggcg cggggaaacc
    26521 gacctcagcg aaatgaaatt attccgggga gcggacccgg cgcaggtcga agagcccccc
    26581 ccgtttgcat ttgcagtccg gatcctcttc gtctccctcg gtgggcaact cgtccctccg
    26641 aaagaacccg ccggacggcg cccttgacgg atcgaacagg tcgtccgccc cccccgtgga
    26701 gacgtcgaga tcgtaggcca aaaaagaaag tatcttccgc ccctccgcgt cccggatctc
    26761 ccattcgggg aaaaaaaagt ccttccgata caggtaccgg agccgatagt cctcccctgc
    26821 ccgacggaaa acggccccct ccagcgtcgg ggtggcggtg tccagataca aggacaactc
    26881 ctcgatatcg cccccccagg ccgaggccga cagccgcacg cggagtttcc ggcacagttt
    26941 cgattccccg ggaaacggtc ccgtcccgtc ctcctccacc cggcttcccg attccatcac
    27001 atggtgcggc aggaaaaacc aaaaggcgtt ttccctcaga tgggcctggg cccaatcccg
    27061 aagcccggga tccttgacgg ccacggcgtt gtcccagacc cccgcccccc cagggccgaa
    27121 aaccgtcagg gtcctcccca gaggagaggt cgtctcttcc cgcccccaca aatcttcacc
    27181 ggtgggccgt ttgaaatact gccgtccatc cacaacgacc cgcaaccctt cgtgagaaga
    27241 tttttcctgg aaccgatagg agaccgaccg ccgctgacga tacgcctcgc ctccccccga
    27301 cgcctccaca caccgggcca acagcgcgtg gcccgacggc accggttcct tccccatggc
    27361 caggaccgcc aggggaagga gcgccaggaa cacggttccg tacggtcttt tcacggatga
    27421 acgggggtgt ttattttttc cgggaacgat tggacctcct ccgggaagtc cgaagtgaag
    27481 aggttttcgt ccaggtaggt attgacgtcc agattgagca gggtgaagac cgccagcggc
    27541 cggccgtcct cgtcgaaaac atcgcggcgg aacggaaaaa ccagggagcg cacctcggcg
    27601 tagtcgctga aaagcagggt ttgcggcggc gcggcggcgg gcggccgata agtggcccct
    27661 tccatccgaa aagtcccgct gtccaccagg aggacgaagg attcttccga cgggaacagg
    27721 ccggcgccct ttcgcgcctc cagccgccgc agaagacgcc cctccacata ccccatgccc
    27781 agatactcca ccgggcacgc cgcctctttc agggaaaagg gggccaatat ccagaaaatt
    27841 ttccgttcca gttcgccccg ggccgccgcc accgcggcgg gctcggtgac ggcccggccg
    27901 tctttccaaa accaggtgcc gctggaagtg aagacggtca cggtccggcc ggagggcgag
    27961 gaaagttcct cgcggccgcg caggccggcc gggtcgtgca gcttgaaccg gtgccggccg
    28021 gtggagagga cgctttttcc gtcgacgaaa cgccgctcgg cgaagtcgta agcgatgctt
    28081 tcgacgcgct ggtatttgga aaatccgccc gcccgctgga ccgatttttc cagataatcc
    28141 gcaccggacg gcggtttttt ggccgccagc aaaaacaccg cccccgccgc cagaccgacc
    28201 ttcagaaatc gtccgctctt catggcggta ttataacagt ttccccttcc ccgtcattcc
    28261 cgtcccgacc gcccttcgga gaagggagtt cccgcaacac atccgctgcg acagcgggac
    28321 ctgtaacaca ggccccgcaa cacgtccgct gcgacggcgg gacctgtaac acaggccctt
    28381 cgttgccgtt aggaaacgtc ccggtttgtc ggacgggacc cgtgtccgat ggttgaggcc
    28441 gggcgttttt cccggggagc cgacgcttta acaaccggga ttcccgccag acgcgtgcta
    28501 tgccgccagg catggcccct gtttgtggaa aggggcggga acgacggatg attgatgtcg
    28561 tcatccacga acgtttctat cggggatcca gggatttttc tgtttttgta tttccgccaa
    28621 tccccggggg aatggcgaca aggcggcgaa agtcactccc cccgcccggc ggaagacacg
    28681 cgcccgcgga ctttttgaaa accgatctcc ccggccttgt cgatgtcggc ttcgacggca
    28741 tccccttccc tgaactcgcc cgccagaagt tttttcgcca agggatcgac gatccatttc
    28801 tgcaggagcc gcttcagcgg ccgcgcgccg taatcgggat cgtagccgaa cttcgccagg
    28861 cgcgccttca accgttccgc cggcacgaga tcgatgcgtt tgtccgacaa acggcggcgc
    28921 accgccgcca gctggatgtc cacgatctcg gcgatgtccg actcgctcag ccgccggaac
    28981 acgatgattt catcgatccg gttcaagaac tccggccgga agtgggcgcg caacgccagc
    29041 gtcacctgcg tattcacctc cttgtcgttt ccggcgcccg aaagatgctg gctcccgacg
    29101 ttggaggtca tcacgatgac ggtgttcttg aagtccacgg tgcgcccctg gccgtcggtc
    29161 aggcggccgt cgtcgagcac ctgcagcagg gcgttgaaca cgtcggggtg ggccttctcg
    29221 atctcgtcga acagcaccac cgcgtagggc cggcggcgca ccgcctcggt gagctggccg
    29281 ccctcctcga agcccacgta gcccggcgga gcgccgatga gccgcgccac cgagtgtttc
    29341 tccatgtatt ccgacatgtc cagccgaacc atggcccgtt cgtcgtcgaa caggaactcc
    29401 gccagcgccc ggcacagttc cgtcttgccc acgcccgtgg gtccgaggaa cagaaaggag
    29461 cccatgggct tgttgggatc ggccagaccc gaacgggccc ggcggatgct gtccgccacc
    29521 gcccggacgg cctcgtcctg gccgaccacc cgccggtgca ggttctcttc catcttcaaa
    29581 agtttggcgg tctccccctc gagcatgcgg ctcaccggaa tgcccgtcca cttggacacc
    29641 accgcggcca cgtcctcctc gtccacgtct tccttgagca tgcgggtgtc tttctgcagg
    29701 ccgtccagtc gttcctgggc cgcctccaat tccttctgca gggcgggcaa acggccgtaa
    29761 cggaactccg ccgccttgcc gaggtccccg gcgcgttcgg cggcgtgttc ctccaaccga
    29821 acctgttcca gccgttcctt tatctcccga atgcggccca gagccgcctt ctccgcctcc
    29881 cactgggtct tcaaggtctt cgacctcttc tgcagatcgg ccagttccga ctccagcttc
    29941 cggaggcgtt cccgcgccgc cgtgtcgctc tccttgcgga gggcctgacg ttcgatctcc
    30001 agctggcggc tcttgcgttc cacctcgtcc agctccaccg gcatggaatc catctccatg
    30061 cgcagccggc tggcggcctc atccaccaaa tcgatggcct tgtccggcag gaaccggtcg
    30121 gcgatgtagc ggtgggacaa ggtggcggcc gccaccaagg cgccgtcctt gatgcgcacc
    30181 ccgtggtgca actcgtagcg ttccttcaat ccgcgcagga tggcgatggt ctgctcgacg
    30241 gtgggctggc ccaccaccac cggctggaac cgccgctcca gggcggcgtc cttttccacg
    30301 tgtttgcggt attcgtccag cgtggtggcg cccacgcagc gcagttcgcc gcgcgccagc
    30361 atgggtttga gcaggttggc cgcgtccacc gcgccttccg ccgcccccgc ccccaccagg
    30421 gtgtgcagtt cgtcgatgaa gagaacgacc cggccgtcgg cggcgacgat ctccttgagc
    30481 acggccttca gccggtcctc gaactcgccc cggtatttgg ccccggcgat gagcgccccc
    30541 aggtccagcg ccaccacacg cttggatttg agcccctcgg gcacgtcgcc gctcaccacc
    30601 cgctgcgcca acccctcgac gatggcggtc ttgcccacgc cgggttcgcc gatgagcacg
    30661 gggttgttct tggtgcggcg cgacaacacc tggatcaccc ggcggacctc ctcgtcccga
    30721 ccgatgaccg ggtccagttt cccccggcgg gccagttcgg tgaggtcgcg tccgtatttt
    30781 tccagcgcgc ggtatttgtc ctcggggttt tgatcggtga cacggtgcgt gccgcgcagt
    30841 tccgacatgg ctttcagcac gccctcccgg gagagcccgg ccttcttcag gagggcggcg
    30901 acgtcatcgc cgtcgacggc cagcagggcc aggagaaaat gttcggtgga gacataatcg
    30961 tcccgaaggg ccgacgcctc ctcctccgcg cgagccaaca tctcccggaa agccgaagag
    31021 gcggacatct ggacgttgcc gcgcacctcc ggcaaacggg ccagggactc gaccacgtcc
    31081 ttttgcagac ggccggcggg cacgccgcat ttttcaacga tcgccggcac caggttgtcc
    31141 ggcacctcga gacacgcgga cagcaggtgc gccgcctcca gacgtggatg gtgttttttc
    31201 tccgccaacc gctgggccga tcgaaggact tcctgcgctt tgatcgtcag tttatccata
    31261 aaataagatt ctcctttata caaaattgat attatttgac aatgtgtttt tactctataa
    31321 tgattcgata gatttttcgc gtcgacccta gttcttccta attttggcac tcatctacat
    31381 agagtgctag tataacacgc cgccgccgaa ttgtcaagag ggggcccgtg gcgtgatgca
    31441 agtgttccag attttgtaaa atgggattga cgtaacatac gtttcaggaa aggaattttt
    31501 catggacatc gtcggcactt tgatcttgtt ggt
//
LOCUS       DAOTFD010000009        31144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_957_length_31144_cov_5.165235, whole genome shotgun sequence.
ACCESSION   DAOTFD010000009 DAOTFD010000000
VERSION     DAOTFD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 31144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31144
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_957_length_31144_cov_5.165235"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="P5079_01245"
     CDS             <1..470
                     /locus_tag="P5079_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRY28642.1"
                     /translation="CSGKNEIFIEVFVGRIRLFVLGAGHVGQKIAELATVAGIPYAVA
                     DDRSEFANRERFPKASAVHVESPDAALRAARVDEKTYVVIVTRGHLLDKECLEAALKT
                     KAAYIGMIGSKSKVPVVISQLNKKGIHPEKDPRVYAPSDWTSAASLPEKSRCR"
     gene            627..4166
                     /locus_tag="P5079_01250"
     CDS             627..4166
                     /locus_tag="P5079_01250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY28643.1"
                     /translation="MHAEFVHLHNHTEYSLLDGGTRLTDEKGNPSEFVKAMAATKYPS
                     LAITDHGNMYGAVEFYRVCTEVGINPIIGIEAYVAPKSRLDRDSRPGEAAYHLTLLAT
                     NETGYRNLLKLSSQAFLEGYYYKPRMDREILSKHSEGLVALSGCLKGEIPSALLADQP
                     QKAVQILDEYRAIFGKDNFFIELMDHGLADQKRVFPMLVDLAKRNEVPTVATNDCHYF
                     RKDDAVAHDVLLCIGTGKTVDDPHRMRYEGQEFYYKSPAEISKQFAEVPEAVKNTLSV
                     AERCHLQLKFDQILLPRYDVPVGETPETYLEKLCLDGLKKRKGHIRPDYKARLDYELS
                     IIRKMGFSTYFLIVWDFIHYSKKAGIPVGPGRGSGAGALTAYALNITNIDPIENGLLF
                     ERFLNPERRSMPDLDIDFSDDGRERVIAYVRQKYGEQCVAQIITFGSMLARLVVRDVG
                     RTLNMPLPDVDKVARLLPKELGTTIHSALESVPELADLYKKDPQVHKLLDLAKRLEGM
                     KRHTGVHAAGIVISAGDMTQHVPLAKGSREVITTQYNDEALLKLGLLKMDFLGLRTLS
                     VIRDASALVKARHDSQFDIDAIPLDDPVTFKLLLQARTAGVFQLESGGMRDLLRKLRP
                     STLSDAVALISLYRPGPMGSGMLDEFVERKHARAKVKYDHPLLEPILKDTYGIIVYQE
                     QVMQIAQALAGYTPGQADVLRKAMGKKIHEILEQQRESFMAGCQKKNVDKKIAQKIFE
                     LIVQFGGYGFNKSHASAYGLVAYQTAYLKANYPVEFMAALLKSEIGHSNLGSKEVESK
                     LVSYLGEAEEMGIEILPPDVQKSEAAFTVENPPADRQRRKIRFGLLAVKNVGEGAVES
                     ILQARREKGPFQSLDDFCSRVDTRQANRKVLESLIKAGAFDSLRPVLGEETEEVRLLE
                     LCRWRSQMLPQVENALAASTRLREETAAGQGALFDMGEIKPRAIPAPQNGGNGTHEEW
                     HEHTLLANEKEVLGFYLSGHPLARYQKELKIYTNRTLGTLPESGKVRVAGMIVNTKKT
                     VTKNGHAMARFKIEDLEGETECVVFPKTYNDVSKFLSAHEMVVVHGEVQLRGDAHEIL
                     VDKVLPLKDAREQLVRRVVLNVSTAGLEDELLKKVQKVCSDHQGKCHLIFRLQTPTRG
                     EYALVTQQRVQATDGFLREMEKMLGRGCWELMA"
     gene            4184..4555
                     /locus_tag="P5079_01255"
     CDS             4184..4555
                     /locus_tag="P5079_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28644.1"
                     /translation="MARILAADDDPETLEYIKMALEMGGHEVTTACDGRQALEAGKAG
                     DFQMYVLDVTMPFLDGYHVAEQLSEKFPERPILLLTSRDYEKDKVAVESCGADAHMSK
                     PFDVNEFLRVIREMTSGKREG"
     gene            4668..5729
                     /locus_tag="P5079_01260"
     CDS             4668..5729
                     /locus_tag="P5079_01260"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY28645.1"
                     /translation="MKKFKVAVVGVGAVGVEMIRVLRQRRFPLDELRVLARSERVIEV
                     DGEKYSVKQTTPESFDGVEVALFAGTEGEKGAAVTFGAEAIQRGAVVIDNGSDFRMDD
                     DVPLVIPEINGDALQNHHGVVANPNCTTATLLMPLGPLHKAFRVKRVIVSTYQAVSGA
                     GGKASEMLRQQAEMVAQGKKVTDFGGFPDRIAFNVLANNWKIEEEGYSNEEWKVMTEA
                     HKILSDKSIQMIVTTVRVPTFVGHGESVYIETEKPISPEAARDVLSQSPGVRVVDEHS
                     PYEGATCPMPIHAAGKDESFVGRIRRDLASKNGLLFWVVGDNLRKGAALNAVQIAEHL
                     IQKNWLKAKTPLKKIAAAK"
     gene            5828..6529
                     /locus_tag="P5079_01265"
     CDS             5828..6529
                     /locus_tag="P5079_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRY28646.1"
                     /translation="MRKSIVYKLVLEYDGTRFSGWQFQPGRRTVQGELEKALRVLFRK
                     RVSAVGASRTDSGVHAMGQVASLRAPGLFHGRLAAALNAHLPSDVSVLSAERAPSSFH
                     ARRDAVEKTYVYRLLARAERPALMRTRAAWLARPLRSGLLRREAERIRRRRNFAAFAG
                     RAGRDRSPLCRLSRLVARKRGDETIFEISGDRFLHRMVRRIVGQLIAAGLGKTRPDPR
                     QTVPPHGLYLKSVRY"
     gene            complement(6523..7491)
                     /locus_tag="P5079_01270"
     CDS             complement(6523..7491)
                     /locus_tag="P5079_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28647.1"
                     /translation="MIANNGSGRSPWWRGPYRDGATFTWDLLYRCNYRCSYCWWEQKK
                     LWGEFSKKYPPVPAQKWIDAWRRLGGRYGRARLDVLGGEPLATPDAAGFFAELSKHHD
                     LVINTNLSPGRKELETLAARCVREAVYINASFHPEFAERESFLEKMRLLKEAGFPAAV
                     FVVSYPPLIPQLPKIREFFSKAGIPVGVQVFQGTYRGRDYPAAFTEAEREALKTDAGL
                     RSSEERWAYRVEERPTRGKLCCAGWFYGNVKADGEVYRCGGTGPGLETEARVGNLFQE
                     DFELFPGPAPCPAGRCFCQEHIYLLEEQEKKCPDLVERWASGKRAQ"
     gene            complement(7478..8992)
                     /locus_tag="P5079_01275"
     CDS             complement(7478..8992)
                     /locus_tag="P5079_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28648.1"
                     /translation="MKITLVLSPMWCREAPPLGLAALKAYLQEQGHNVRCFDFNNEFC
                     LRTSEKWKPYWGSEHGTAVWTKEDFAGTVISAHEALLDEAVGQIAAREAKLVGFSVYA
                     TNALLSLEMARRLKKKAPDVFVVFGGPWVTQEREKVLKHPAVDAAVLGEGEGSLAELA
                     RSMDQGRPLTEVPGLYLRADGDTGVSFKAGTELASLDALPFPDFSDFPVGTYDRPRRL
                     LAYFSRGCFKKCVFCDVPVHWKKWRCRSGRRVFEDLRHQLTRYPDIRAIEFCEATLNS
                     DLRALEDFCDLVIADKKSDRSAFRRIFWFGFAHVRPEMDRPFLEKMARAGCHHLRLGI
                     ESGSQKVVDSMKKGYRLADAERLLADMRSAGIKALLTFMVGFPTETEEDFQGTLDFLR
                     RNARLIERVTPNESFTAIFPGTPLHRQAESFGVELGAHSDHWRTKDGSNDFHVRLARH
                     ERFCREAAALGLALSPSSTKVARYRDDFLADYEETASSHGWTGPREADEEDDRK"
     gene            9298..9792
                     /locus_tag="P5079_01280"
     CDS             9298..9792
                     /locus_tag="P5079_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRY28649.1"
                     /translation="MTKPTDLSNVKWNAATLKDVRLVPLTTHQDDRGYLVEIAHSTDP
                     YLPKFGQVYLVGNPARGAVRAFHKHAKLWDLFSITHGSAKFVLVDDRPESPTRGEANV
                     FVLSHKDHSLLVVPPGVYHGWMSLEDDTQMISTASEVYDRGRPDEVRIPPDSFGDVWT
                     VKGR"
     gene            9800..10879
                     /locus_tag="P5079_01285"
     CDS             9800..10879
                     /locus_tag="P5079_01285"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY28650.1"
                     /translation="MGTVLVAGGAGYIGSILCRELLELGFSVRVADRFFYGNAGVVGI
                     KDRIEILEKDLRQIAESDLKGVDACINLAGLSNDPTAEYNPDANWDMNALGAEHLAQV
                     CRSAGVWRYILASSASVYDRGGQQEEQDVVLTEESEVFPKAAYAVSKLEGEKRVLALK
                     NDRFSPIILRKGTVHGFSPRMRYDLVVNTFVKDALGSGTITLFYGGEMWRPLVEIRDV
                     ARAYIAALQAPLEKVRGEIFNVVEKNYRISELALHVQGRLEKLGIRANVAVDYAYRSV
                     RSYRVSSKKIRQTLGWAPLVAVEDSVELMVKNIRECGYTNFDYPRYYNIEWMKLLQEA
                     QTVSSRSHPQNIFKVPDETTPPAAS"
     gene            10876..11781
                     /gene="rfbD"
                     /locus_tag="P5079_01290"
     CDS             10876..11781
                     /gene="rfbD"
                     /locus_tag="P5079_01290"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRY28651.1"
                     /translation="MSGVSVLRRSSTAMKILLIGSAGQLGTDLQKTNPGHPLVPLTHA
                     DIDITDAAESEKILRRHAPDVVINTAAYHRTDDCEDFPEKAFAANLLGPRLLARVCAE
                     TGARLVHYSTDYVFDGKKRLPYRESDPPFPLSVYGISKLGGEFAVRSQADRNIVIRSA
                     SLFGTAGSSGKGGNFVETMIRRSREGLSTEVVDDMIMSPTYTLDLAQMTWALLAKEVP
                     GGLYHIANGGTCSWYEFTRKIFALLRLETPLKPIRSADRPAKIRRPLYSALGSERLPE
                     LGIPPLRVWQEALRAYLIEKGHLTP"
     gene            11955..12173
                     /locus_tag="P5079_01295"
     CDS             11955..12173
                     /locus_tag="P5079_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY28652.1"
                     /translation="MDIKDLSSYLGIGKSKIYSLIRQRKIPASRIGRQYRFSKEVIDS
                     WLKERLITGPQESPLPLFEKKNPQPPQA"
     gene            12269..12490
                     /locus_tag="P5079_01300"
     CDS             12269..12490
                     /locus_tag="P5079_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28653.1"
                     /translation="MAQKIAKAGVKREEGYLYFIDKQGDISRAKMARGGKKGGKPQKV
                     KKVGVKKEKGYLYFMDKQGDVSRAKMVRR"
     gene            complement(12628..12984)
                     /locus_tag="P5079_01305"
     CDS             complement(12628..12984)
                     /locus_tag="P5079_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRY28654.1"
                     /translation="MNSFSEPSGGSPAPGCPRCGKISCACLPERGAAVVPPPLRQKEP
                     VTLRLDRNGRKGKTVTLVEGLKMHPEGKVALLKKFQKLCGAGGALKAGTPEIQGDHRG
                     RIRTELEVLGFRVRVI"
     gene            13128..13550
                     /locus_tag="P5079_01310"
     CDS             13128..13550
                     /locus_tag="P5079_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY28655.1"
                     /translation="MSAFGFAVLAAFLWGSAAICEKMGLVSGADPLTGLWARCIGVVI
                     GTAALSFLIPQLPVRLAAAGWKSFFWLAGGGLIASVLGQFFFYRALKVGEIGRIATVG
                     GSWPLVAFLLSLLFLHEAPTPRKLAGVVLVFLGVTLLK"
     gene            13630..14127
                     /locus_tag="P5079_01315"
     CDS             13630..14127
                     /locus_tag="P5079_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRY28656.1"
                     /translation="MAQEFSFDVVSNVDLQAVDNAVNTANREITTRFDFKGSCSRIDL
                     DKKTALITLHSDDDGKLKSVIDILQTRLVKQSISLKALDFQKIEPAEGGTVRQTAKIV
                     QGIASEKAKEIVRVVKDSKVKVTPSIQGDHVRIAGRAKDDLQTAIALLRSKDFGIPLQ
                     FTNYR"
     gene            14150..16501
                     /locus_tag="P5079_01320"
     CDS             14150..16501
                     /locus_tag="P5079_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY28657.1"
                     /translation="MTPDPGIFSRSPSPTTSARRPKFLWRAAVAGLLVVAGGAVVITW
                     LRVPNDHRVNFSFPDIGNESKSVIVDFIERVRECRYLATDIVEAAGGAGLLATDPKSA
                     GPRVVLASVEAEGQTVRRRSLLAPAGAAYVYSLTVPPAADLEFYYAVSPPKPSPTGGV
                     RFVVELTRPDNQTQILFSTTASAAGPPTFRQRFSRRLRRWIGRDIADGPDPWEWAHLS
                     LAPWAGQAVKLSFRTEPGSTGLLPEEPAGPDVFFPQAYWGEPVLKASPPAAAKGSAGI
                     NLILLVTDTLRWDAVVAHGNSGRLTTSHDRFLRQAMDFQRVYSNSTSREYSLASLLRS
                     KSLTTGDVPDKNPNGGLSAEGEDGTADAASLAGALKRRGYRTAFVGNARPGIDSGFDE
                     AHLFPPNGYGPALVSRRAVEWLTQNGRGGPFLLVVYFGNDAASAAPPPRFWASAAVES
                     LPRSDRGRLTQRARIAYADEYLGRFLDILDAYEFWPRSLVSLVSLRGEGVDRAALAEP
                     EIRVLWALRHRGLPEGVSVSEPVQLADAAPTLLRYLNAEVPAEFQGRNLHVAAGGFSR
                     PEEPARILLQSDEAKGVILNYHHKYVRRKLASPQADDAESIYDLWADPAEEKNLVRRD
                     RGLLAKVRRALEELSPDRLETRLLFWDLGDRPVRGSLETGAGEIRSFVSSGAAGRRGA
                     NFYDFEILSSSGEVRFEIWPPMASYVLRVKLKDEYFPAERIRVSRFGLPLFERSGVEW
                     YDYNVFPWMTGLSFPQKGETGPLLFMGRCPPKKEESSRPGEVF"
     gene            16502..18733
                     /locus_tag="P5079_01325"
     CDS             16502..18733
                     /locus_tag="P5079_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY28658.1"
                     /translation="MRPELKNTIRNAFRYCGERFLSGGLEAARWIERESLLSLEARTG
                     AATFAGLFFLSLLQSRFWRPFSLPGTDGLLLSLRSFWPEALTLHGILFFASLALGTVV
                     GILFRNAWDILELFAPALHRGRFSRSTRIAAAAAWSLFFHLTFLTRDMAAHPALYQNT
                     FYDAGGALRGFMTFAVERCPAWLGGLPGAAVAMVLFLAVVDWSRRSFLWFMAFPVPAR
                     LAAAVMTGGLTLASLGVWGVIRLQAPRNDGPNVLVLMVDSLRSDALENPRFPRLSALA
                     KAGRTYSRCYPNLAATPAALTTFFTGQTPLTHGLRHSFPSPEKTRLGPNSLLPLLKQR
                     GYFTAALTDSNGDFFSSLSENFDTLRAADLSGRRLAQQRALRAHVHLLPYLSGKTGRR
                     LFPALRNLPELSDPALLAEEATSLLGRLRFRKNFFLAVYFSALHSPYAVSSRTALESL
                     EKDYRGPCKYAAPADEGEIATPAEQARVRRLYGANVRAVDAAVGTLLDNLAARHLGEN
                     TLVLLWSPYGEQLYERGLGRGHGRHLRGEETLAAPFVVVEPRQRTPPRWIREPVRGQD
                     VAPTLLDILDIPPPETMEGFSLRRRDFSQPPDEFFLVYCETDVWLSGADGDPRFPSRI
                     PYPSLSQMLSFGPRGNDRPSLLLNWEDDVLVGKQRLVQLGTERLLYIPTREGVEYEYY
                     DLASDPHGMHNLAGSSGRAEREQEFREILFRFLSRETGWRPQNGFWIPEAFLNDPDGD
                     PLL"
     gene            18730..19782
                     /locus_tag="P5079_01330"
     CDS             18730..19782
                     /locus_tag="P5079_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28659.1"
                     /translation="MKKIAPPRTGVSYFGNRFLPHALRDLDVVADCCDYVVHAVNETD
                     FYFHKAATEKIFSASRRKGLEVWADPWGLGGVFGGEALSKFLLDHPDDWQVLSDGRRV
                     PAACPNRDSFKNFVREWVTLVRDSGAEVIFWDEPHIYFHWNKEWEGTYACVCPACRDL
                     YRQRFGGDMPAQLDDSAREFRRRTLSSFLDDLTRFAAHQRLKNALCLYAFEGYAEYDR
                     IWKQLAALPKLDVFGCDPYWRWSPFPWKTLLSKKAGPLRTPAAYVKYFSEKVLAETRD
                     AGKGSQIWLQAMRFPRGAEKEILPAAQAAAEAGVTHLAAWSYDGGALLDTVLSENPER
                     VWKETARAFRHLKGAR"
     gene            19784..20734
                     /locus_tag="P5079_01335"
     CDS             19784..20734
                     /locus_tag="P5079_01335"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRY28660.1"
                     /translation="MPALLPEKKILVVGPGALGTYFAARLGHAFPRLWVLDHREGRAQ
                     QLSEAGFHVRGVDSYDWFAPERHVAARPDGWPAMDFIFFLVKAPALAKAAGNCRRLTG
                     KDTAWVLLQNGWRAEEELKTEKKRIVLGITGEAVTLESTGRVFHAAVGTTLLDAKTKR
                     AAEVSRLLAAAGFKVELTKDFDSARWNKLLLNAVINPIGALAGVPNGRLAENPLKELA
                     DGLTLECVRLARRAGRTTPPAEAQRRLRETIAQTAANKNSLWQDLLRGRPTERPYLLG
                     PFLDLARRHKTPAPLLQSLDRLLQTVENFQQHEAARRGEP"
     gene            20731..21591
                     /locus_tag="P5079_01340"
     CDS             20731..21591
                     /locus_tag="P5079_01340"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY28661.1"
                     /translation="MTSVFHILNPSAGQRGWVRAVTEGQTAMAPWAWVSREHPLHTET
                     LVDWALREGTKRLVVWGGDGTFHRVVETLWRRRALEKVELALVPCGTCNDLARRLNLS
                     KYYWRRWEASSPEGRPARLAVGRLAWENFSTVFVNNAGFGRSRESYEAKQGPWGVLKS
                     FEPIGVAAEWDAGRLDGRYYMMLACNAPYFSGGLHFEKTVSPETGTLQVYFVPATSKA
                     RLAWRLLRGRLGQPMFDTKTTKISTRSLRLRTDRPVWPQADGEPPPAAVSSLELGLLP
                     EQIRLWCPAL"
     gene            21599..22150
                     /locus_tag="P5079_01345"
     CDS             21599..22150
                     /locus_tag="P5079_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRY28662.1"
                     /translation="MKPRAYLDIETDWQKRITIIGVYRPGADTQQWVRPDLSADGLRE
                     FLQGIETLYTYNGARFDLPVLRDQLDIDLAKEYRHHDLMYDCWSRCLFGGLKKVEKRL
                     GITRDTEGLDGRDAVLLWDRYVKGEKDLLELLLRYNREDVINLEALAVTLGLVPREEG
                     ELPAGFRVPEVKGRDDSGDCIES"
     gene            22165..23031
                     /gene="folD"
                     /locus_tag="P5079_01350"
     CDS             22165..23031
                     /gene="folD"
                     /locus_tag="P5079_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRY28663.1"
                     /translation="MSAKILDGKKAAAKVLENVRQKAAELARRGVTPGLATVLVGDDP
                     ASAVYVRQKIKACEAHGLKSVHVPLPADVSEVALLEKIRELNRSPEVHGIIVQLPLPK
                     HIHADRALLAVDPRKDVDGLHPVNQGQWMRLKSWKEILASGRPLPCTPAGVMELLEQN
                     GVRVAGRRATVIGRSTLVGKPLALMLLSADATVTLCHSRTTNIAAVCREADILIAAIG
                     SPRFVKSSMVKRGAVVVDVGSNRTEKGTTGDVDFERVRPLAKAITPVPGGVGPMTVAM
                     LLWNTVRSAEQA"
     gene            23246..23443
                     /locus_tag="P5079_01355"
     CDS             23246..23443
                     /locus_tag="P5079_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRY28664.1"
                     /translation="MKGKVKWFNAAKGYGFITTEEGNDVFVHYSAITGDGYKSLDEGQ
                     AVEFEVTSGPKGPAAANVKKA"
     gene            23584..24216
                     /locus_tag="P5079_01360"
     CDS             23584..24216
                     /locus_tag="P5079_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28665.1"
                     /translation="MRLPDRLGRFEGVTDLPLIIVASNAVVSLFEWVRPGLTPMLTLS
                     PAAVWAGEFWRLLTFLFVPPPLSPLFLLFWLYILYIYADALETEWGSFRFTVYYLLGA
                     LATAAVGFFPAHGTIPNVYLNASLFLAFATLFPTFELLLFFVLPVQVRYLGYLTWAWL
                     GWSFLTGGLLTRLAILAGLINYILLLGPDLRERLMLRWQTYRNRRRWKTR"
     gene            complement(24225..24416)
                     /locus_tag="P5079_01365"
     CDS             complement(24225..24416)
                     /locus_tag="P5079_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28666.1"
                     /translation="MSAEKRIPKERRLVERRRQRRRQMNLPVSVERRSGSDRRGPPRR
                     RGIRRTGDTYLGKRKKHSR"
     gene            24522..25907
                     /locus_tag="P5079_01370"
     CDS             24522..25907
                     /locus_tag="P5079_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28667.1"
                     /translation="MPQPPDLGLAYIGAVLKNAAYPVKVLDLNWELYYARPPETPSPW
                     DGYDDDALEKFGADLFRNHLPQIAARIDELARDGFNVFGLSVWASNKTSSLALARTIK
                     ARHPASVVIFGGPECMPRWSGEAFAAEPALDAVVYGEGEETVLEILRSIEESGAVRPA
                     PGCYARESGKMRNGGPRPSPVFSALPIPDFNDYDLSLNNGRLGITFTRGCAHRCRFCS
                     LTGYIAHLKTRPAEHIFFEIKQRLERHRVRCFEERCPSVSSNLPELKKVCEMIVSHRL
                     KFTWTGQCVFHPNLSYDLLGLMHRAGCHHLVFGLESGSQKVTDLMGRGYRLTDAENNL
                     RDLKKAGIHVTLNVLVGYPGETEEDFRETLRFLEKNRDHIDSLGAVSTLWISPYALVA
                     NQTERLNIIAPQGNIYGRSWRTKDDSNTPEIREDRRNRLLQRAGSLNLVSPAHLHVLQ
                     TSETSHSKTPK"
     gene            26098..27048
                     /locus_tag="P5079_01375"
     CDS             26098..27048
                     /locus_tag="P5079_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28668.1"
                     /translation="MGPDQTIDVSTVRYKLSQIFMLLGLGIFVVSAGFATRGREPFAT
                     WFFDFAWWSYLLFIDGWVYRHRGESLLLNHPGRFLFLACWSVLLWFLFEAFNLRLQNW
                     AYVGLPAALPLRWFGYAIAFATVAPAILETADLVETAQFIKEGRVRPLGWRRSVGPYF
                     MALGALMLLLPLLWPRFFFPLVWGGFLFLLEPLNARWGVPSLITDWREGRLHRFWILL
                     ISGFFCGVLWEWWNSWSGARWVYSVPWVSGCKIFEMPLLGYLGFPPFAVSVFVMTVFA
                     VTLWEKSPRPVRALLVLAAAAAVLLMCAAVDQFTVKGFQP"
     gene            27045..28010
                     /locus_tag="P5079_01380"
     CDS             27045..28010
                     /locus_tag="P5079_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY28669.1"
                     /translation="MSRLIQRALVTGGAGFLGSHLCDHLLRRGVSVICVDNFLTGNPD
                     NIAHLTGHPRFRFIRHDVTQDLYLHEPIDAVLHFASPASPRDYLEHPIKTLKVGALGT
                     HKMLGLAKTKKSRFLLASTSEVYGDPLVHPQQESYWGNVNPVGPRGVYDEAKRYAEAL
                     CMAYHRVHGVRVRIARIFNTYGPRMQKHDGRAIPEFVMAALRGKSLPVFGEGLQTRSF
                     CYIDDEAEGLLRLLYSDCPGPVNIGNPAEITILDIARMILKLTGNRGRIVHKPLPVDD
                     PKVRQPDIRQAKRVLGWTPRVPLETGLKNTIAWVKKNYLPRGARR"
     gene            28050..30041
                     /locus_tag="P5079_01385"
     CDS             28050..30041
                     /locus_tag="P5079_01385"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY28670.1"
                     /translation="MKKIRIVSLALALAVSFAVLVVFVRGLFQGLESQSIDFRFKIRG
                     DRVPNENVVIITADEKSIAELGRWPWSRATHGRLIKWLTDAGAKAIAFDILFTEPDNE
                     HPQDDVALGRLASKSGRVVFGMFFQKGEDNLPAAPLLPIPPLRNDRIHMGSVNFWPEV
                     DGVTRKLPVWMEYDGQFYPSLSLSVLAVAEQKDPQTLFQELNLPTEDLWNEFSLNFAG
                     GFQMFPYFSYADVLKGRVSPAVFKDKIVFVGGTATALFDFKAVPNVPLFPGLEIHAVA
                     VDNYLNGNHLRTINPLWTILLILVFGLGCGLLLSRVPAWAGALSTAAWIFGYYSVCQV
                     LFSKKHIVLDFVAPATVVLGTYVVVLFYRFLTEQREKRWIKGTFSQYLSPKVIEVITS
                     DPGRLRLGGEEREMTVFFSDLAGFTSISEAMKPPDLVAVLNEYLTAMSEIILKYDGVV
                     DKYIGDAIMAFWNAPVDQPRHAVLACLTALEQMERLAVLQRGFAERKLPAIDCRIGIN
                     TGNMVVGNMGSKNRFDYTVMGDSVNLASRLEGANKPYHTRIMISEFTYEKAKNDIEVR
                     QLDLLRVKGKAIPIKVYELIAAKGRLTDNQKKAFASYEEGLAFYLDRKFEKAAALFKK
                     TQALLPHDGPSDVYLNRCAGYLAAPPPKDWDGVYVMTTK"
     gene            30073..30771
                     /locus_tag="P5079_01390"
     CDS             30073..30771
                     /locus_tag="P5079_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HRY28671.1"
                     /translation="MEVFWRLLFGHLLGDFTLQTNYVAAWKRRGPAGLLFHVGTHIVL
                     YVFLAWPYLGTTWIHIGPWALNGWAGLGLIFVSHVFEDQWRIWSVTAQRAPDNLLFYI
                     WDQVIHIVILFALAPVTSDRIIENTWPVMGCLVVAATHFTTVSVFFIEKDLFNMKFPP
                     TREKYGAMAQRFLVFLCLLLPSAWAWLVLGAVGLQVLVRRQIPSQAVSKTGLVLGNGL
                     ALLAGLLARRLYFA"
     gene            30853..>31144
                     /locus_tag="P5079_01395"
     CDS             30853..>31144
                     /locus_tag="P5079_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28672.1"
                     /translation="MAKLDKKLLAAQALSARPRPAAKPTDPVLVIVPHVPVPDRIPED
                     RAADNLIKLLREHFRVVIAADRPAPLEDPYRRHFLMEGIEIVGGQQDFPQEQP"
BASE COUNT         5853 a        10445 c         9219 g         5627 t
ORIGIN      
        1 aatgttccgg aaaaaacgag atatttatcg aggtcttcgt cgggcggatc cgactgttcg
       61 tcctcggcgc cgggcacgtg ggccagaaga tcgccgaact cgcgaccgtc gcggggatcc
      121 cctacgccgt ggccgacgac cgatcggagt tcgcgaaccg ggagcgcttc cctaaagcct
      181 cggcggtgca cgtcgagtcc ccggacgcgg ccctgcgcgc cgcccgggtg gacgagaaga
      241 cctacgtggt catcgtgacc cggggtcacc tgctggacaa ggaatgcctg gaagcggcgc
      301 tgaaaaccaa ggcggcctac atcggcatga tcggctccaa aagcaaggtc cccgtggtca
      361 tctcccagct gaacaaaaag gggatccatc cggaaaagga cccccgcgtc tacgcgccgt
      421 cggactggac atcagcggca agtctcccgg agaaatcgcg gtgtcggtga tggcggaaat
      481 cctcaaagtc agttacggcc ggagcggaaa acacctgcgc gacctctaac gggcggccga
      541 agaagctcat atccgccccc caacaatctt tccaaacgtc tcccggagag ctataatttt
      601 ctgaagtcct ccgaaaaaaa gaatccatgc acgccgagtt cgttcatctt cacaaccaca
      661 ccgaatacag cctcttggac gggggcacgc gcctcacgga cgaaaaaggc aacccgtccg
      721 aattcgttaa agccatggcg gccaccaaat atccctcgct ggccatcacc gaccacggca
      781 acatgtacgg cgcggtggaa ttctaccggg tgtgcacgga agtcggcatc aatcccatca
      841 tcggcatcga ggcctacgta gcgcccaaat cgcggctcga ccgcgacagc cgtcccggcg
      901 aggccgccta ccacctcacc ctgctggcca ccaacgaaac cggctaccgc aacctcctga
      961 aactcagctc ccaggccttt ctggagggct actactacaa accccgcatg gaccgggaga
     1021 tcctctcgaa acattccgag gggctggtgg ccctctccgg ctgcctgaag ggggagatcc
     1081 cgtcggcgct cctcgccgac cagccgcaga aggcggtcca aattctggac gagtaccgcg
     1141 ccatcttcgg caaggacaat ttcttcatcg aactgatgga ccacggactc gccgaccaaa
     1201 aacgcgtttt ccccatgctc gtcgatctgg caaaacggaa cgaggtcccc acggtggcca
     1261 ccaacgactg ccactatttc cgaaaggacg acgcggtcgc ccacgacgtg ctcctgtgca
     1321 tcggcaccgg aaaaaccgtg gacgacccgc accgcatgcg gtacgagggg caggagttct
     1381 actacaaatc gccggcggag atatcgaagc aattcgccga agtgccggag gcggtgaaga
     1441 acacgctgtc ggtggccgaa cgctgccacc tgcagttgaa attcgaccag atcctgctgc
     1501 cgcggtacga cgttccggtg ggagagaccc ccgaaaccta cctggaaaaa ctctgtctcg
     1561 acgggctcaa gaaacgcaaa ggccacatcc ggccggacta caaggcccgg ctcgactacg
     1621 agctgagcat catccgcaag atggggttct ccacctactt cctcatcgtc tgggatttca
     1681 tccactactc caaaaaggcg gggatcccgg ttgggccggg gcgcggatcg ggcgccggcg
     1741 cgctgaccgc ctacgcactc aacatcacca acatcgaccc catcgagaac gggctgctct
     1801 tcgagcggtt cctcaacccc gagcgccgca gcatgcccga cctggacatc gacttttccg
     1861 acgacggccg cgaacgcgtg atcgcgtatg tgcgccagaa atacggcgag caatgcgtgg
     1921 cgcagatcat cactttcggc tccatgctgg cgcgtctggt ggtgcgcgac gtgggccgca
     1981 ccctcaacat gcccctgccc gacgtggaca aggtggcccg cctgctcccc aaagaactcg
     2041 gcaccaccat ccactccgcc ctggaatccg tgccggaact ggcggacctc tacaagaaag
     2101 acccgcaggt gcacaagctc ctggacctgg cgaaacggct ggagggcatg aaacgccaca
     2161 ccggcgtgca cgcggcgggc atcgtgatct ccgccggcga catgacccag cacgtgccgc
     2221 tggccaaggg ctcgcgcgaa gtcatcacca cccagtacaa cgacgaggcc ctgctgaagc
     2281 tggggctctt gaagatggac ttcctggggc tgcggaccct ctcggtgatc cgcgacgcct
     2341 cggcgctggt gaaggcccgc cacgattccc aattcgacat cgacgccatc ccgctggacg
     2401 accccgtcac cttcaagctc cttctgcagg cgcgaacggc gggagtgttc cagctagaat
     2461 ccggcggcat gcgcgacctc ctgcgcaaac tccgccccag caccctctcc gacgcggtgg
     2521 cgctgatctc cctctatcgt cccggcccca tgggctccgg catgctcgac gagttcgtcg
     2581 agcgaaaaca cgcgcgcgcc aaggtgaaat acgaccatcc gctgctcgaa cccatcctca
     2641 aagacactta cggcatcatc gtctaccagg aacaggtcat gcagatcgcc caggcgctgg
     2701 cggggtatac ccccggccag gccgacgtgc tgcgcaaagc catgggaaaa aagatccacg
     2761 agatattgga gcagcagcgc gagtcgttca tggccgggtg ccagaaaaag aacgtggaca
     2821 aaaagatcgc ccagaagatc ttcgaactca tcgtccagtt cggcggatac ggtttcaaca
     2881 agagccacgc ctcggcctac ggcctggtgg cctaccagac ggcgtatctc aaggcgaact
     2941 acccggtgga attcatggcc gcgctgctca aatcggaaat cggccactcc aacctcggtt
     3001 ccaaggaagt ggagtccaag ctcgtcagct atctcggcga agcggaggag atggggatcg
     3061 agatcctccc tcccgacgtc caaaaatccg aggcggcctt caccgtggaa aatccgccgg
     3121 cggaccggca acgccgcaag atccgcttcg gcctgctggc ggtgaagaac gtcggagaag
     3181 gcgcggtgga atccatcctg caggcgcggc gggagaaggg gccctttcaa tcgctcgacg
     3241 atttctgttc ccgcgtggac acgcggcagg ccaaccgaaa agtgctggaa tctctcatca
     3301 aggccggcgc gttcgattct ctccggcccg tgctcggcga ggagacggaa gaggtgcggc
     3361 tgctggaact ctgccgctgg cgttcccaga tgctgcccca ggtggaaaac gcgctggccg
     3421 cctccacgcg cctgagggaa gaaaccgccg ccggacaggg cgctcttttc gacatggggg
     3481 aaatcaagcc gcgcgcgata cccgcgccgc aaaacggcgg gaacggcacc cacgaagaat
     3541 ggcacgaaca cacactcctc gccaacgaga aggaggtgct cggcttctat ctttccggac
     3601 accccctggc ccggtatcag aaggaattga agatatacac caaccgcacg ctcggcacgc
     3661 tgccggaatc gggcaaggtg cgcgtggcgg gaatgatcgt gaacacaaaa aagaccgtca
     3721 ccaaaaacgg ccacgccatg gcccgcttca aaatcgagga cctggaaggc gaaacagaat
     3781 gcgtcgtctt cccaaaaacc tacaacgacg tctccaaatt cctcagcgcc cacgagatgg
     3841 tggtggtgca cggcgaggtg cagctccgcg gagacgccca cgagatcctg gtggacaagg
     3901 tgcttccttt aaaggatgcg cgggagcagt tggtccggcg ggtggtgctg aacgtctcca
     3961 ccgcggggct ggaggacgag ctgctgaaaa aagtccagaa ggtctgctcc gaccaccagg
     4021 gcaaatgtca cctcattttt cgcttgcaga ccccgacccg cggggagtat gctttagtga
     4081 cccaacaacg cgtgcaggcc accgacggtt tcctgcgcga gatggaaaaa atgctcggcc
     4141 gcggctgctg ggaactcatg gcctgagccg gaaaggactt cgcatggcac ggatattggc
     4201 ggcggacgac gaccccgaga cgctggaata catcaagatg gcgctggaga tgggcggcca
     4261 cgaggtcacc acggcctgcg acggccggca agcgctggag gccggaaaag ccggcgactt
     4321 ccagatgtat gtgctcgacg tgaccatgcc gttcctcgac ggctaccatg tggccgaaca
     4381 gctctccgaa aagtttccgg aacgccccat ccttctcctc acgagccgcg attacgaaaa
     4441 ggacaaggtg gccgtggaat cctgcggcgc cgacgcccac atgtccaaac ccttcgacgt
     4501 gaacgagttc ctccgcgtga tccgggagat gacttcggga aaacgggagg gctgaccgtt
     4561 tccgactttt tttgattttt gtataataaa aaaacggcgt cccgaacgcg gggcgccttt
     4621 tttaatcgat ccggcggcct ctccgccgat ccatgaagga gtcgctcgtg aaaaagttta
     4681 aagtggccgt tgtcggtgtc ggcgccgtcg gcgtcgagat gatccgggtt ttgaggcagc
     4741 gccggttccc cctggacgaa cttcgtgtgt tggcgcgcag cgagcgcgtc attgaggtgg
     4801 acggggagaa atattccgtg aagcaaacca cgccggaatc cttcgacggc gtggaggtgg
     4861 cccttttcgc cgggaccgag ggggaaaaag gcgccgccgt caccttcggc gccgaggcca
     4921 tccaacgcgg cgcggtggtg atcgacaacg gctccgattt ccgcatggac gacgacgtgc
     4981 cgctcgtgat ccccgagatc aacggggacg ccctgcagaa ccaccacggc gtcgtcgcca
     5041 atcccaactg caccaccgcg acgctcctca tgcccctcgg gcccctgcac aaggccttcc
     5101 gggtaaagcg cgtcatcgtc tccacctacc aagccgtctc cggcgccggc ggcaaagcct
     5161 ccgagatgct ccgccagcag gcggagatgg tcgcccaagg gaaaaaagtc acggacttcg
     5221 gcggctttcc ggaccgcatc gccttcaacg tccttgccaa caactggaag atcgaggagg
     5281 agggatacag caacgaggaa tggaaagtga tgaccgaggc ccacaagatc ctctcggaca
     5341 agtccatcca gatgatcgtc accacggtgc gggtgcccac gttcgtcgga cacggcgagt
     5401 cggtctacat tgaaacggaa aaaccgatct cgccggaagc cgcgcgggac gtcctctccc
     5461 aatcgcccgg tgtccgcgtg gtggacgaac actcccctta cgaaggggcg acctgcccga
     5521 tgcccatcca cgccgccggc aaggacgaat cgttcgtcgg ccgcatccgc cgcgacctgg
     5581 ccagcaagaa cgggcttctc ttctgggtgg tgggggacaa cctccgcaag ggagcggctc
     5641 tcaacgcggt gcagatcgcc gaacacctca tccagaagaa ctggttgaaa gccaaaacac
     5701 cgctcaagaa aatagcggcg gcgaaatagc ggctccgccc tcgcgcgaca acccatcgcc
     5761 cccgcgcccc cctgctcgac ggaggaactc tcctgccgcc cgacggacgg cggccccctc
     5821 aatgaccatg agaaaatcca tcgtctataa gctggtgttg gaatacgacg gcacgcggtt
     5881 ttccggctgg cagttccagc ccggccggcg gaccgtgcag ggagagcttg aaaaagcgct
     5941 tcgggttttg tttcggaaga gggtttcggc ggtgggggcg tcccgcaccg atagcggcgt
     6001 tcacgccatg ggacaagtgg cgagcctccg ggcgccgggc ctgttccatg gacgtctcgc
     6061 cgcggccctg aacgcccacc tgccgagcga cgtttccgtt ctctccgcgg aacgcgcgcc
     6121 atcgtcgttc cacgcccggc gagacgccgt cgaaaaaacc tacgtctatc gtcttctcgc
     6181 ccgggcggag aggcccgcgc tcatgagaac acgggccgcc tggttggcga ggccgctccg
     6241 atcgggcctc ctgcggcggg aggcggaacg gatacggcgc cgacgcaatt ttgccgcctt
     6301 cgcggggcgc gccgggcgag atcgttcacc cctctgccgg ctgagccggc tcgtcgcgcg
     6361 taaacggggg gacgaaacca ttttcgaaat ttccggggat cgcttcctgc accgcatggt
     6421 ccgccgcatc gtaggccagc tcatcgccgc cgggctgggc aaaacccgtc ccgatccgcg
     6481 ccaaaccgtc ccgccccacg gactctatct caaatccgtc cgctactgag cgcgttttcc
     6541 ggaagcccag cgttccacga ggtccggaca ttttttttcc tgttcctcca aaagataaat
     6601 atgttcctgg cagaaacagc ggccggccgg acaaggggcc ggcccgggaa aaagctcgaa
     6661 atcctcctgg aagagattcc cgacgcgcgc ttccgtttcc aaccccggcc cggttcctcc
     6721 gcaccggtag acctctccat ccgccttcac gttgccgtag aaccagcccg cgcaacaaag
     6781 tttcccgcgc gtgggccgct cctccacccg gtaggcccac cgctcttccg acgaccggag
     6841 cccggcatcc gttttcagcg cctcccgctc cgcctccgtg aaagccgccg gatagtcccg
     6901 gccccggtaa gtcccctgaa aaacctgaac cccgacgggg atccccgctt tcgagaaaaa
     6961 ttcccgaatc ttcggcaact gcgggatgag cggcgggtag gaaacaacga acaccgccgc
     7021 cgggaaaccg gcctctttca gcaaccgcat tttttccagg aaactctccc gctccgcgaa
     7081 ttcgggatgg aaactggcgt tgatgtatac ggcctcccga acgcagcggg ccgccagggt
     7141 ctccaactct ttccggccgg gagacaggtt cgtgttgatg acaaggtcgt ggtgtttcga
     7201 gagttccgcg aaaaacccgg cggcgtcggg agtggcgagc ggttccccgc cgagaacgtc
     7261 gagccgggcg cggccgtatc ggccgcccag ccggcgccag gcatcgatcc atttctgcgc
     7321 ggggacgggc ggatattttt ttgaaaattc cccccagagt ttcttctgct cccaccaaca
     7381 atagctgcag cgatagttgc accgatagag caggtcccag gtgaaggtgg ccccgtcccg
     7441 gtagggaccc cgccaccacg gggagcggcc cgacccgtta tttgcgatca tcttcttcat
     7501 cggcttcccg agggcccgtc cacccatgag aagacgccgt ttcttcgtaa tccgccaaaa
     7561 aatcgtcccg atagcgggcg actttggtcg acgaagggga gagggccagg ccgagcgccg
     7621 ccgcctcccg gcagaaccgc tcatgccgcg ccaggcggac atggaaatcg ttgctcccgt
     7681 cctttgtccg ccaatgatcc gaatgcgcgc ccaactcgac gccgaacgat tcggcctgcc
     7741 gatgcagcgg cgtgcccggg aagatggcgg tgaagctctc gttcggagtg acccgctcga
     7801 tgaggcgggc gttgcgccgg aggaaatcca gcgtcccctg aaaatcctcc tcggtctccg
     7861 tggggaatcc gaccatgaac gtcaggagcg ccttgatgcc ggccgaccgc atgtccgcca
     7921 aaagccgttc cgcgtcggcc agacggtatc ctttcttcat ggaatccacg accttttgag
     7981 atccgctctc gatgccgagg cggaggtggt ggcatccggc ccgggccatc ttttccaaaa
     8041 acgggcggtc catctccggc cgcacgtgcg cgaaaccgaa ccagaatatc ctccgaaacg
     8101 ccgaccggtc gctttttttg tccgcgatga cgaggtcgca aaaatcttcc agggcgcgga
     8161 ggtccgagtt caaggtcgcc tcgcagaact caatggcgcg gatgtccgga taccgcgtca
     8221 actggtgtcg gaggtcctca aagacgcgcc ggcccgagcg gcagcgccat tttttccaat
     8281 gaaccggcac gtcgcagaaa acgcatttct tgaaacagcc gcggctgaaa tacgccagca
     8341 gccggcgcgg ccggtcgtag gtgccgaccg ggaaatcgct gaaatccggg aacggcaggg
     8401 cgtccaagga ggccaactcc gtccccgctt taaaggagac ccccgtgtcc ccgtcggcgc
     8461 gaagatagag ccccgggacc tccgtcagcg gccggccctg gtccatggaa cgcgccagtt
     8521 cggccagcga gccctcgcct tcgccgagga cggcggcgtc caccgcggga tgcttcagga
     8581 ccttttctct ttcctgggtg acccacgggc cgccgaaaac gacaaagaca tcgggggcct
     8641 tcttcttcag ccgacgcgcc atttccaggc tgagcaaagc gttggtggca tagaccgaga
     8701 aaccgacgag tttcgcctcc cgcgccgcga tctgtccgac ggcctcgtcc aacagtgctt
     8761 cgtgggccga gatcaccgtc cccgcaaaat cctccttcgt ccaaacggcg gtcccgtgtt
     8821 ccgaacccca atagggtttc catttctccg acgttctcag gcagaactca ttgttgaaat
     8881 cgaaacatcg gacgttgtgg ccctgttcct ggagataggc cttcaaggcc gccaggccga
     8941 gcggcggggc ctcgcggcac cacatgggac tcaacaccag ggtgatcttc attggacggg
     9001 ccggggcttt cggaaatacg gcgggcggag cgcgcggggg agccgcacgg cgccgtgggg
     9061 ggccgcgccc ggaggcgtcc tgcgtgagcg atcgggaagc atcaggtggt gcgtatcggt
     9121 tccccgtcca tgaatttttt cgagcgggcc gggcggccgc gggccttccg gcgcgttctt
     9181 ttcattttgc ttgaaaaagg tccggggcgc aagccctgtt gaagcggggc tttggatatt
     9241 atagagtctc ccgacctccc caaagaggcc gacatcgaca ggcatttttc cgcaaacatg
     9301 acaaaaccga cagacctctc caacgtaaaa tggaacgcgg ccacgctgaa agacgtgcgc
     9361 ctggtgccgc tcaccaccca ccaggacgac cgcggctacc tggtggagat cgcccattcg
     9421 accgacccct acctgccgaa attcgggcag gtctacctcg tcggcaaccc ggcgcggggc
     9481 gcggtgcggg cgttccacaa gcacgccaaa ttgtgggatc tcttctccat cacccacggc
     9541 tcggccaagt tcgtcctcgt ggacgaccgg ccggagagcc cgacccgcgg cgaagcgaac
     9601 gttttcgtcc tgtcgcacaa ggaccacagc cttctggtgg tgccgccggg ggtgtaccac
     9661 gggtggatgt ccctggagga cgacacccag atgatcagca ccgcctcgga agtctacgac
     9721 cgcgggcggc cggacgaggt ccggatcccg cccgattcgt tcggcgacgt ctggacggtg
     9781 aaagggaggt agctcgctca tgggaacggt cctcgtcgcc ggcggcgccg gttatatagg
     9841 aagcatcctt tgccgggaac tcctggaact ggggttcagc gtccgcgtgg ccgaccggtt
     9901 cttttacgga aacgccgggg tggtcgggat caaggaccgg atcgagattt tggaaaaaga
     9961 cctccggcag atcgcggagt ccgatttgaa gggggtcgac gcctgcatca atctggccgg
    10021 cctctccaac gacccgacgg cggaatacaa cccggacgcc aactgggaca tgaacgccct
    10081 gggcgcggag cacctggcgc aggtgtgccg ttcggcgggc gtgtggcgct acatcctcgc
    10141 ctcctccgcc tccgtctacg accgcggcgg ccagcaggag gaacaggacg tggtgctcac
    10201 ggaagaatcg gaggtgttcc ccaaggcggc gtacgcggtg tccaagctgg agggggagaa
    10261 acgggtgctg gccctgaaga acgaccgttt ttcgcccatc atcctgcgca agggcacggt
    10321 gcacggtttc tcgccccgca tgcgctacga cctggtggtg aacacgttcg tcaaagacgc
    10381 cctcggttcc gggaccatca ccctcttcta cggcggagag atgtggcggc cgctggtgga
    10441 gatccgcgac gtggcccgcg cctacatcgc ggccctgcag gcgcctctgg aaaaagtgcg
    10501 cggcgagatt ttcaacgtgg tggagaagaa ctaccgcatc tccgaactgg ccctgcacgt
    10561 ccaggggcgg ctggaaaaac tgggcatccg ggcgaacgtg gccgtggact acgcctaccg
    10621 cagtgtacgg agctaccgcg tctcatcgaa aaagatccgg caaaccctgg gctgggcccc
    10681 cctggtggcc gtggaagatt ccgtggaact gatggtgaaa aacatccgtg aatgcggcta
    10741 caccaacttc gactaccccc gctactacaa catcgaatgg atgaagctcc tgcaggaggc
    10801 gcagacggtc tcctcccgga gccatcccca gaacatcttt aaagtcccgg acgaaacaac
    10861 gccccccgcc gcctcttgag cggggtctcc gttttgcgcc gctcctccac cgccatgaaa
    10921 atccttctca tcggctccgc cggacagctc ggcaccgacc tgcaaaagac gaaccccggc
    10981 catcccctcg tccccctgac ccacgcggac atcgacatca cggacgccgc cgaatcggaa
    11041 aaaatcctcc gccggcacgc cccggacgtg gtcatcaaca ccgccgccta tcaccgaacc
    11101 gacgactgcg aagatttccc ggaaaaggcg tttgcggcca acctgctcgg gccgcgcctc
    11161 ctggcccgag tctgcgccga aaccggcgcg cggctggtcc actacagcac cgactacgtg
    11221 ttcgacggca aaaaacggct gccctaccgg gagagcgacc cgcctttccc gttgagcgtg
    11281 tacggcatct ccaaactcgg cggcgaattc gcggtgcggt cgcaggccga caggaacatc
    11341 gtcatccgtt cggccagcct cttcgggacg gccggctcca gcggcaaggg cggcaacttc
    11401 gtggagacca tgatccggcg ttcccgggag ggactctcga cggaggtggt ggacgatatg
    11461 atcatgtctc cgacgtacac cctggacctg gcgcagatga cctgggcgct gctggcgaag
    11521 gaagtccccg gcggcctgta tcacatcgcc aacggcggca cctgctcgtg gtatgaattc
    11581 acccggaaga ttttcgccct cctgcgcctc gaaaccccct tgaaacccat ccgctccgcg
    11641 gaccggcccg cgaagatccg tcgaccgctc tattccgccc tcggctccga gcgcctgccg
    11701 gagttgggca tcccgcctct gcgcgtctgg caggaggcgc tccgcgcgta tctcatcgaa
    11761 aaaggccacc tgaccccata gactcgacgc gcccacaaaa ttcttctccc gcccacggcc
    11821 tctcccttaa taggaagagg ttttttattt tcttgacttc cccccaacga ttcggtacca
    11881 tttagcacaa ttaatcaact aaaaatatta ggtgatactt aatggcagaa gaaggaaagt
    11941 ctataaaaga ggtcatggac atcaaggacc tctcgtccta tctggggatt gggaaatcca
    12001 agatctacag ccttatccga caacgcaaga ttccggcgtc gcggatcggg cggcagtacc
    12061 gtttttcgaa agaggtcatc gattcctggc tgaaggaacg gctgatcacg ggaccgcagg
    12121 agtcgccgct gccgctcttc gaaaagaaga accctcagcc gccgcaagcg tagcaaaacg
    12181 aaaataatct tgagtttccg tccggaaatg ctaaagtacg gcggcgttcg atttgagcaa
    12241 aatattcacg gaaggaggtg aaaaagacat ggcgcagaaa attgcaaagg ccggagtcaa
    12301 acgtgaggaa ggttacctgt atttcatcga taaacagggg gacatctcgc gggcgaagat
    12361 ggctcgtggc gggaaaaagg gcggcaaacc gcagaaagtg aagaaagtgg gagtcaagaa
    12421 ggaaaaaggc tacctctact tcatggacaa gcagggtgac gtctcccgcg cgaagatggt
    12481 tcgccgataa aaaagatatc tctgctgcgc gacttcccac tgacaacacg gcgcgcgcag
    12541 ggaagcaagt tcctcggccc ctgagtaagg ggccagaacc ctgtctcttt ccggaacttt
    12601 tcggatgggg gcagggttct tgatttttta gatcacccgg acccgaaacc ccagcacttc
    12661 cagctcggtc cggatccgcc cgcgatggtc gccctgaatc tccggggtgc ccgccttcaa
    12721 cgcgccgccc gcgccgcaca acttttggaa ttttttcagc agggccactt tgccttccgg
    12781 atgcatcttc agtccctcca ccagcgtgac ggtcttgcct ttccggccgt tccggtccaa
    12841 gcgcagcgtc accggctcct tctgccggag cggcggcgga acaacggccg ccccccgttc
    12901 cggcaaacag gcgcaggaaa ttttaccgca ccggggacag cccggcgcgg gcgaaccgcc
    12961 cgaaggttcc gaaaaactgt tcaagcgccc ctcccaataa tctgtattga aattcgtcca
    13021 aagaagctaa aatactcccg ctcttctagg ataccagatg ccgggtcacg gttcaaaccc
    13081 ggcccaaaac cggcaacaac gcccttgcgg gcggagggga agacatcatg agcgcattcg
    13141 gctttgcggt gctggcggct tttctttggg gatcggcggc gatctgtgaa aaaatgggtc
    13201 tggtgtccgg cgccgacccc ctcaccggcc tgtgggcgcg gtgcatcggg gtggtgatcg
    13261 gcacggcggc gctcagcttc ctcatcccgc aacttcccgt gcgcctggcg gccgccggat
    13321 ggaaatcgtt cttctggctg gccggcggcg ggttgatcgc cagcgtattg ggtcaatttt
    13381 tcttttatcg cgcccttaag gtcggcgaga tcggccgcat cgccaccgtc ggcggctcct
    13441 ggcccctggt ggcgttcctg ctcagtctcc ttttcttgca cgaagccccc acgccccgta
    13501 aactggcggg tgtggtcctt gtcttcttag gcgtcacgct gctgaaatag cgctgccgcg
    13561 cggacgccct tccgccggtc tgaatggaaa ccgttttcca tttattttca tgaacgagga
    13621 ggctgccgta tggctcagga attctcattc gatgtggtgt ccaacgtgga ccttcaggcc
    13681 gtggacaacg ccgtcaacac cgccaatcgg gagataacga cccggttcga tttcaagggc
    13741 agttgctcac ggatcgacct cgacaaaaaa accgccctca tcaccctcca ttcagacgac
    13801 gacgggaagc tcaaaagcgt catcgatatc ctccagacgc gccttgtgaa acagtccatc
    13861 tccctcaaag cgctcgattt ccagaagatc gagccggccg aaggcggcac cgtgcggcag
    13921 acggccaaaa tcgtccaggg catcgcctcc gaaaaagcca aagagatcgt tcgcgtggtc
    13981 aaagactcca aagtcaaggt gacgccgtcc atccaggggg accacgtccg catcgccgga
    14041 cgcgccaagg acgacctgca aaccgccatc gccctgctgc gatccaagga cttcggcatt
    14101 ccgctgcaat tcacgaacta tcgctgaacc tcccccgccg ccgcgcccca tgacacccga
    14161 cccggggatt ttttcccgat ccccctcccc aacgacctcc gcacgcagac cgaagttcct
    14221 gtggcgggcc gcggtcgccg gcctcctggt cgtcgccgga ggcgcggtcg tcatcacctg
    14281 gctccgcgtc ccgaacgacc atcgggtgaa tttcagtttc ccggatatcg gaaacgagtc
    14341 caaatccgtc atcgtcgact tcatcgaacg ggtgcgggaa tgccgctacc tggcaaccga
    14401 catcgtcgag gcggcgggcg gagcgggcct cttggcgacc gatccaaaat ccgccggccc
    14461 ccgcgtggtc ctcgcctccg tggaggcgga agggcaaacc gttcgacgtc gttccctcct
    14521 ggcgccggcc ggcgccgcct atgtttactc cctcaccgtc ccgccggccg ccgacctgga
    14581 attttattat gcggtctctc ccccgaaacc ctcgccgacc ggaggcgtgc gattcgtcgt
    14641 ggaattgacg cgcccggaca accagacgca gattctcttc tccaccaccg cctccgccgc
    14701 cggccccccg acgttccgcc aacgtttctc ccgccgcctc cggcgttgga tcggacgcga
    14761 catcgccgac ggcccggacc cgtgggagtg ggcccacctc tcccttgccc cctgggcggg
    14821 acaggcggtg aaactcagct tccgaacgga accgggctcg accggacttc tccccgagga
    14881 gccggcgggg cccgacgttt tctttccgca ggcctactgg ggcgaaccgg tcctgaaagc
    14941 gtcgccgccg gcggcggcca aggggtcggc ggggatcaat ctcatccttc tggtgacgga
    15001 cactttgcgc tgggatgccg tcgtcgcgca cgggaacagc ggccgattga cgacgagcca
    15061 cgaccgcttt ttgcggcaag ccatggactt tcagcgcgtt tattccaact ccacttcccg
    15121 cgaatactcc ctggcctccc tgctccgatc aaaaagtctg acgaccggcg atgtgccgga
    15181 caaaaacccg aacggcggcc tctcggccga gggagaggac ggcacggccg acgccgcctc
    15241 gctggccggc gccctcaaac ggcgcggata tcgaacggcc tttgtgggaa acgcccggcc
    15301 ggggatcgac tccggtttcg acgaagcgca tctttttcct ccgaacggtt acggacccgc
    15361 cctcgtctcc cggcgggcgg tggaatggct gacccaaaac ggccgcggcg gcccatttct
    15421 cctggtggtc tattttggaa acgacgccgc ctccgccgcg cctccgccgc ggttctgggc
    15481 ctccgccgcg gtcgaatctc tcccgcgttc ggaccggggc cggctgaccc agcgggcgcg
    15541 catcgcctac gccgatgaat acctgggccg tttcctcgat atcctggacg cctacgagtt
    15601 ctggccccgt tccctcgtct cgctcgtttc ccttcgcgga gaaggcgtcg accgcgccgc
    15661 cttggcggaa ccggagatcc gcgttctctg ggccttgcga catcgcgggc tgccggaggg
    15721 ggtttccgtt tcggaaccgg tccagttggc cgatgccgcc cccacgctcc tccgctacct
    15781 gaacgccgag gtgcccgcgg aattccaggg ccggaacctc cacgtcgccg ccggcggttt
    15841 ttcccgcccg gaagaacccg cgcggatcct cctgcaaagc gacgaggcca agggagtgat
    15901 cctgaattac catcataagt acgtgcgccg gaaactcgcc tccccgcagg ccgacgacgc
    15961 ggaaagcatc tacgatctct gggcggaccc ggccgaagaa aaaaacctcg tccgccgcga
    16021 tcgcggtctc ctggcgaagg tccgtcgcgc cctggaagag ctctcgccgg accgtctgga
    16081 aacccggctg ctcttctggg acctcgggga ccggcccgtg cggggaagcc tcgagaccgg
    16141 ggccggagaa attcgctctt ttgtgtcttc cggcgccgcc ggacggcgcg gggccaactt
    16201 ctacgacttc gagatcctct cctcttccgg ggaggtccgt ttcgagattt ggccgccgat
    16261 ggcctcttac gtcctgcgcg tcaaactcaa agacgaatat tttcccgccg aacgaatccg
    16321 cgtgtcccgc ttcggtctgc cgctcttcga gcgcagcggc gtggagtggt atgactacaa
    16381 cgttttcccg tggatgaccg gcctttcgtt tccacaaaaa ggagaaaccg gcccgttgct
    16441 cttcatgggt cggtgtcccc ccaaaaaaga agagagctcc cggccgggag aggttttctg
    16501 aatgcggccc gaacttaaga acaccatccg gaacgccttc cgctactgcg gcgagcggtt
    16561 cctttccggc ggactggaag ccgcgcgatg gatcgaacgc gaatcactcc tctcgctgga
    16621 ggcgcgcacg ggcgcggcga cgttcgccgg cctctttttc ctctcgctgt tgcaatcccg
    16681 cttttggcgg ccgttctctt tgcccggaac ggacggcctc ctcctctccc tccgctcatt
    16741 ctggccggag gcgctgaccc ttcacgggat cctcttcttc gcgagcctgg ccctggggac
    16801 ggtcgtgggg atcctgtttc gaaacgcctg ggacatcctg gaactgttcg ctcccgccct
    16861 ccatcggggc cggttctccc gctccacgag gatcgcggcc gccgcggcct ggtccctctt
    16921 tttccatctc acttttctca cccgggacat ggccgcccat ccggcgttgt atcagaacac
    16981 gttttacgac gcgggggggg ccctccgcgg attcatgacg ttcgcggtcg aacgttgccc
    17041 ggcctggctc ggcggtctgc cgggggccgc ggtggcgatg gtgcttttcc tggccgtggt
    17101 ggactggtcg cgccgttcct ttctctggtt catggccttc cccgtccccg cccggctggc
    17161 ggcggcggtc atgacgggcg gcctgacgct ggcctctctc ggtgtttggg gcgtgatccg
    17221 tctccaagcg ccccgcaacg acggccccaa cgttctcgtt ctcatggtgg actccctgcg
    17281 ttcggacgcg ctggaaaacc ctcggttccc tcggctgtcc gcgctggcga aggccgggcg
    17341 gacgtacagc cgctgttacc cgaacctggc cgcgaccccg gccgccctca cgaccttctt
    17401 caccggtcaa acgccgctga cccacggcct gcgccactcc tttccgtctc ccgaaaagac
    17461 ccggctcggc cccaactccc tcctccccct gttgaaacaa cgcggctact tcaccgcggc
    17521 cctcaccgac tccaacgggg atttcttctc ctccctgtct gaaaatttcg atacgctgcg
    17581 ggcggcggat ctcagcggcc ggcggctggc gcaacagcgg gccctccggg cccacgtcca
    17641 tcttctcccg tatctttccg ggaaaaccgg ccggcggctt tttccggccc tgcgaaacct
    17701 gccggaactc tccgacccgg cgctcctcgc cgaagaggcg acgtcccttc tgggccggct
    17761 gcggttccgg aaaaatttct tcctggcggt ttacttttcg gccctccact ccccctacgc
    17821 cgtttcttcg agaaccgccc tggaatcgct ggagaaagat tatcgcggcc cctgcaaata
    17881 cgccgcgccg gccgacgagg gggaaatagc gactccggcg gaacaggccc gggtccggcg
    17941 cctttatggg gccaacgtcc gcgcggtgga cgccgcggtg ggaaccctcc tcgacaacct
    18001 ggccgcccgg cacctggggg agaacaccct cgtgctcctc tggtcgcctt acggggaaca
    18061 actctacgag cggggtctgg gccgagggca cggccggcac ctgcgcggcg aggaaacgct
    18121 cgcggcgccg ttcgtcgtcg tggaaccgcg ccaacgcacc ccgccgcgct ggatacggga
    18181 acccgtgcgc ggccaggacg tggcccccac cctcctcgat atcctggaca tccctccgcc
    18241 cgaaaccatg gaagggttct cgctgagacg gcgcgacttc tcgcagccgc cggatgaatt
    18301 tttcctggtg tattgcgaaa cggacgtctg gctctccggc gccgacggcg atccccgttt
    18361 tccgtcgcgc atcccctatc cgtctctttc acaaatgctt tccttcggcc cgcgcgggaa
    18421 cgaccgccct tccctgctct tgaactggga ggacgacgtc ttggtcggaa aacaacgcct
    18481 cgtccaactg gggacggaac gcctccttta tatccccacg cgggaagggg tagaatatga
    18541 gtactatgac ttggcctcgg acccccacgg catgcacaat ctcgcgggct catcgggccg
    18601 cgcggagcgg gagcaggagt tccgggaaat ccttttccgc ttcctctcgc gcgaaaccgg
    18661 atggcgcccc cagaacggtt tttggatccc ggaagccttt ttgaacgatc cggacggaga
    18721 tcccctgcta tgaaaaaaat cgccccgccc cggaccggag tcagctactt cggcaaccgg
    18781 ttcctcccgc acgccctgcg cgacctcgac gtcgtggcgg actgctgcga ctacgtggtc
    18841 cacgcggtca acgagacgga cttttatttc cacaaggcgg ccacggaaaa aatcttctcg
    18901 gcctcccgcc ggaaagggct ggaggtctgg gcggacccgt ggggactcgg cggcgtgttc
    18961 ggcggagaag ccctctccaa atttctactg gaccaccccg acgactggca ggtcctgtcg
    19021 gacgggcggc gcgttccggc ggcctgcccc aacagggact ccttcaagaa cttcgtccgc
    19081 gaatgggtga ccctcgtgcg cgactccggc gccgaggtga tcttttggga cgaaccgcac
    19141 atttatttcc actggaacaa agaatgggag gggacctacg cctgcgtctg cccggcctgt
    19201 cgcgacctgt accggcaacg cttcggcggc gacatgcccg cgcaactcga cgacagcgcc
    19261 cgcgagttcc gtcgccggac gctctcctcg tttctggacg atctgacccg attcgccgcg
    19321 caccagcgcc tgaagaacgc cctctgcctc tacgctttcg aagggtacgc cgaatatgac
    19381 cgcatctgga aacaactggc cgccctcccg aaactcgacg tcttcgggtg cgacccctat
    19441 tggcgctgga gccccttccc ttggaagacc ctcctctcca aaaaagccgg tcccctgcgc
    19501 acccccgccg cctacgtgaa atatttttcg gaaaaagtgc tcgcggagac gcgggacgcg
    19561 ggcaagggga gccagatatg gctgcaggcg atgcgtttcc cgcgaggcgc cgagaaggaa
    19621 atcctgcccg cggcgcaggc cgccgccgag gccggggtga cccacctggc ggcctggagc
    19681 tacgacggcg gcgccctcct cgataccgtc ctttcggaaa accccgaacg cgtctggaaa
    19741 gaaaccgcgc gggcgttccg ccacctcaaa ggagcccgct gacatgccgg cgcttttgcc
    19801 ggagaaaaaa attctcgtcg tcgggccggg ggcgctgggc acctacttcg cggcccggct
    19861 gggacacgcc ttcccgcgcc tctgggtgct ggaccaccgg gaaggccgcg cgcaacaact
    19921 ctccgaggcc ggcttccacg tgcggggcgt ggactcctac gactggttcg cgccggaacg
    19981 gcacgtcgcc gcccgccccg acggctggcc ggcgatggat ttcatctttt ttctcgtcaa
    20041 agcgccggcc ttggcgaaag cggccgggaa ctgccggcgc cttaccggaa aggacaccgc
    20101 ctgggtcctg cttcagaacg gctggcgggc ggaggaggaa ttgaagacgg agaaaaaacg
    20161 catcgtcctg gggatcaccg gcgaagccgt gacgctggaa agcacgggcc gcgtttttca
    20221 cgccgccgtc ggcacgaccc tcctcgacgc caaaaccaag cgcgccgcgg aagtctctcg
    20281 cctgctggcc gcggcggggt tcaaggtcga actcacgaag gatttcgatt ccgcccgctg
    20341 gaacaagctc ctcctcaacg ccgtcatcaa ccccatcggg gcgctggccg gcgtgccgaa
    20401 cggccggttg gccgaaaacc ccctgaaaga actggccgac gggctcaccc tcgaatgcgt
    20461 ccggctggcc cggcgggccg ggcgaacgac cccgccggcg gaggcgcaac gccggctccg
    20521 ggaaaccatc gcccaaacag cggccaacaa gaattccctg tggcaggacc tcctccgcgg
    20581 acggccgacg gaacgcccct atctcctggg tccctttctc gacctggcgc gccggcacaa
    20641 aacaccggcc cccctccttc agtcgcttga ccgattgctc caaacagtgg agaatttcca
    20701 gcaacacgaa gcggcgcgga ggggcgaacc atgacatccg ttttccacat cctcaacccg
    20761 tcggccggcc agcgcggctg ggtccgcgcg gtcaccgaag gccaaaccgc catggctccc
    20821 tgggcctggg tgagccggga acatcccctg cacaccgaaa cgctcgtgga ctgggccctg
    20881 cgggaaggga cgaaacgcct ggtggtctgg ggtggcgacg ggacgttcca ccgggtggtg
    20941 gagacgctgt ggcggcgccg agcgctggag aaggtggagc tggccctggt gccctgcggc
    21001 acgtgcaacg atctggcgcg gcggctgaac ctttccaaat actactggcg ccgatgggaa
    21061 gcctcgtcgc cggagggccg gccggcccgg ctggccgtcg gccggctggc ctgggagaat
    21121 ttttcgacgg tgttcgtcaa caacgccggg ttcggccgct cacgtgaatc ctatgaggcg
    21181 aagcaggggc cgtggggcgt tttgaaatcg ttcgagccca tcggcgtcgc cgcggaatgg
    21241 gacgccggcc ggctggacgg ccgctactat atgatgctgg cctgcaacgc cccctatttt
    21301 tccggcggac ttcactttga aaaaacggtc tcccccgaaa cgggaacgct ccaggtctat
    21361 ttcgtgccgg ccacctccaa ggcgcggctg gcgtggcgac tgctgcgcgg acgactgggc
    21421 cagccgatgt tcgatacgaa gaccacgaag atctccaccc gctcgctccg gttgcgcacc
    21481 gaccggcccg tctggcccca ggcggacggg gaacctcctc cggcggccgt gtcgtccctg
    21541 gaactcggcc tcctcccgga acagatccgt ctctggtgcc ccgcgttgta agatgaagat
    21601 gaaaccgcgc gcctatctgg acatcgaaac ggactggcag aaacggatca ccatcatcgg
    21661 ggtctaccgc cccggcgccg acacccagca gtgggtccgt ccggacctct ccgccgacgg
    21721 cctccgcgaa tttttgcagg gcatcgagac gctctacacc tacaacgggg cccggttcga
    21781 cctccccgtc ctccgcgatc agctcgacat cgacctggcg aaggaatacc ggcatcacga
    21841 cctcatgtac gactgctggt cccgctgtct cttcggcgga ctgaaaaaag tggagaagcg
    21901 cctcggcatc acccgggaca ccgagggcct cgacggccgc gacgcggtgc tcctgtggga
    21961 ccggtacgtc aaaggggaaa aagacctgct ggagctcctg ctccgctaca accgagaaga
    22021 cgtgatcaac ctggaagccc tggccgtgac gctggggctg gtgccgcgcg aagagggcga
    22081 actgcccgcc ggattccgcg tccccgaagt caaagggcgg gacgactccg gcgactgcat
    22141 cgaatcgtga cgaggagaaa aaccatgagc gccaaaatat tggacggcaa aaaagcggcg
    22201 gccaaggtct tggaaaacgt gcgccaaaag gccgccgaac tcgcccggcg cggcgtgacg
    22261 cccgggctcg ccaccgtgtt ggtgggcgac gaccccgcct cggcggtgta cgtccggcag
    22321 aagatcaagg cctgcgaggc ccacgggctc aaatccgtcc atgtgccgct ccccgccgac
    22381 gtctccgagg tggccctgct tgagaagatc cgggagctca accggtcccc ggaggtgcac
    22441 ggcatcatcg tccagttgcc tttgccgaaa catatccacg cggaccgggc cctcctcgcg
    22501 gtcgacccgc gcaaggatgt ggacgggctc catccggtca accagggaca atggatgcgg
    22561 ctgaaaagct ggaaagagat cctcgcgtcc ggccgccccc tgccctgcac cccggcgggg
    22621 gtgatggagc ttctggaaca gaacggcgtc cgcgtggcgg gccgccgggc gacggtgatc
    22681 ggccgttcca ccctggtggg aaaacccctc gccctcatgc tgctctcggc cgacgccacc
    22741 gtcacgctct gccactcccg cacgacaaac atcgccgcgg tctgccgcga ggcggacatc
    22801 ctcatcgccg ccatcggctc cccccgtttc gtcaaaagct ccatggtcaa gcgcggcgcc
    22861 gtggtggtgg acgtcggcag caaccgcacc gaaaagggga cgaccgggga cgtggacttc
    22921 gagcgcgtcc gccctttggc caaggccatc acgcccgtgc ccggcggcgt gggccccatg
    22981 acggtggcca tgcttctctg gaacaccgtc cgctccgccg aacaggcctg aggcccccgg
    23041 cggcggcaat taattccacg cccccccctt gattaatttt gcccggcgtg ctataattct
    23101 tttgtaagcg tgaatgtacc gttccaactt tatcacagtc aaacgtagac gttgaaccgc
    23161 ggaggcctcc tcagtggccg tcgcggattt tttttaaggt tgcggacttc atattttttc
    23221 ttacaggggt aggtgattaa cagccatgaa gggaaaagtc aagtggttca acgccgcgaa
    23281 ggggtatgga ttcatcacca ccgaagaggg gaacgacgtg ttcgttcact attcggcgat
    23341 cacgggagat ggatacaaat ccctcgacga aggacaggcg gtggagtttg aggtgacctc
    23401 gggccccaag ggaccggccg ccgcaaacgt caagaaagcc taatcttcaa aaccccccgt
    23461 cggcggacgt tcgttctccg gcggggggtt tttctatttc cgtacgcagt tcgatctcgg
    23521 acgccgccat gcgcacacgc aactcaaaaa cacatcgaaa aaaatccgcc ggacgcaagc
    23581 gccatgcgcc ttcctgaccg gctgggaagg ttcgaaggcg tcaccgacct tcccctcatc
    23641 atcgtcgcgt cgaacgccgt cgtctccctg ttcgaatggg tccggcccgg cttgacgccg
    23701 atgctgaccc tctccccggc cgccgtctgg gccggagaat tctggcggct cctgacgttc
    23761 ctcttcgtac cgccgcccct gtcgcccctc tttctcctgt tctggctcta catcctctac
    23821 atttacgccg acgccctgga aacggagtgg gggtcgttcc gattcaccgt ctactacctg
    23881 ctcggcgccc tggccacggc ggcggtgggg tttttcccgg cccacgggac gatccccaat
    23941 gtctatctca acgccagcct cttcctggcc tttgcgaccc ttttcccgac gttcgaactg
    24001 ctgttgtttt ttgttctgcc cgtgcaggtg cgctatctgg gatatctcac gtgggcgtgg
    24061 ctgggctgga gttttcttac ggggggcttg ttgacccgcc tggcgatcct ggcgggactc
    24121 atcaactaca tcctgcttct cggcccggac ctccgggaac ggctgatgtt gcgttggcag
    24181 acctaccgga accggcgacg ctggaaaacc cggtaacgcc cgtactagcg ggaatgtttt
    24241 tttcttttgc cgaggtaggt atcgccggtg cgacggatgc cgcgacggcg cggcgggccg
    24301 cggcggtccg aaccgctccg ccgttcgacc gagaccggaa gattcatctg ccggcggcgt
    24361 tgccgccggc gctccaccag tcgtcgttct ttggggatcc ttttttccgc tgacatctcg
    24421 aacctccgaa aattctgtcc catcttagca cattagattt gctattttct agctctccca
    24481 tgaaaccgaa ggtttgcctg atccgttgtc cggcgatcac catgccgcaa ccccccgacc
    24541 tcggcctggc ctacatcggc gccgtgctga agaacgccgc ctatccggtc aaggtcctgg
    24601 acctgaactg ggaattgtat tacgcccggc ccccggaaac cccatccccc tgggacggct
    24661 acgacgacga cgccctggag aaattcgggg cggacctctt tcggaaccac ctgccacaaa
    24721 tcgccgcccg catcgatgaa ctcgcccgcg acggcttcaa cgtcttcggc ctttccgtgt
    24781 gggcctccaa caaaacaagc agcctggccc tggcgcggac catcaaggcc cggcatcccg
    24841 ccagcgtcgt cattttcggc gggccggaat gcatgccccg ctggtccgga gaggcgttcg
    24901 ccgccgagcc ggccctcgat gcggtggtct acggcgaggg ggaggaaacg gtccttgaga
    24961 tattgcgctc catcgaagaa agcggggcgg ttcggccggc gcccggctgt tatgcgcggg
    25021 aatccggaaa gatgcggaac ggcggcccac ggccgtctcc cgtgttctcg gcgctgccga
    25081 tccccgattt caacgattac gatctttcgc tcaacaacgg ccggctgggg atcacgttca
    25141 cccgcggatg cgcgcaccga tgtcgtttct gttccctgac gggctacatc gcgcacttaa
    25201 aaacccggcc ggccgaacac atcttttttg agataaaaca acgcctggaa cggcaccgcg
    25261 ttcggtgttt tgaggaacgg tgcccttcgg tcagctccaa cctgccggaa ttgaaaaaag
    25321 tctgcgaaat gatcgtctct caccggttga aattcacctg gaccggacaa tgtgtttttc
    25381 atccgaacct gtcctacgac ttgttggggc tgatgcaccg ggcgggctgt catcacctgg
    25441 tctttggtct ggaaagcggc tcccaaaagg tgacggacct gatgggccgc ggctatcgcc
    25501 tgaccgacgc ggagaataat ctgcgcgacc tgaagaaagc agggatccac gtgaccctga
    25561 acgtgctcgt cggctatccg ggagaaactg aagaagattt tcgcgagacg ttgcggttcc
    25621 tcgaaaaaaa cagggaccac attgactctc tcggggccgt cagcaccctc tggataagcc
    25681 cctacgccct cgtggcgaat cagacggaga gactgaacat catcgcgccc cagggaaata
    25741 tctacgggcg ctcctggcgg acaaaagacg actcgaatac ccccgaaatc cgcgaggatc
    25801 ggcggaaccg gctgctccaa cgagcggggt ctttgaatct ggtatctccg gcccatcttc
    25861 atgtgctcca aacgtccgaa acgtcccatt caaaaacccc gaaataaccg cctaacgcct
    25921 tacgctcccc cgggccccgc ggcgggctcc gtcctatagc ttttttgacg gatattgggt
    25981 agaatgacct gcccctctcg cgaggggttt ttcgtcagtg aaaaaccggg attccggtca
    26041 aaatttcgtg aatgcatccc gttcacatgt caggattcgt tgcgcaaaca cgtcatcatg
    26101 ggccccgacc aaacaatcga cgtttccacc gttcgttaca aactaagcca gatcttcatg
    26161 ctgctcgggc tggggatatt cgtcgtctcc gccggcttcg ccacccgggg acgggaaccg
    26221 ttcgccacct ggttcttcga ttttgcctgg tggagctatc ttcttttcat cgacggctgg
    26281 gtctaccggc atcgcgggga gtccctcctg ttgaaccatc cgggccggtt cttgttcctg
    26341 gcgtgctggt ccgtcctcct gtggttcctc tttgaagcgt tcaacctccg gctccagaac
    26401 tgggcctatg tgggcctccc ggcagcgctt cctctccggt ggttcgggta tgcgatcgcc
    26461 ttcgccaccg tggcgccggc catcctcgag accgccgacc tggtggaaac cgcgcaattc
    26521 atcaaagaag ggcgcgtgcg gccgctgggc tggcgacgca gcgtgggccc ctatttcatg
    26581 gccctcggcg cgctcatgtt gctgttgccg ctcctctggc cccggttctt cttccccctg
    26641 gtttggggcg ggttcctgtt cctgctggaa cccctcaacg cccgctgggg cgtgccgtcc
    26701 ctcatcacgg actggcgcga ggggcgcctc caccggttct ggatccttct catcagcgga
    26761 ttcttctgcg gggtcctctg ggagtggtgg aactcctggt ccggcgcccg ctgggtctat
    26821 tccgtcccgt gggtctccgg gtgcaagatt ttcgagatgc ctcttctggg ttacctgggc
    26881 ttcccgccgt tcgccgtttc ggtctttgtc atgaccgtct tcgccgtgac gttgtgggaa
    26941 aaatcgccgc gcccggtccg ggccctcctc gtcctcgccg cggccgcggc cgtcctcctg
    27001 atgtgcgccg cggtggacca attcaccgtg aaaggattcc aaccatgagc cgtcttatcc
    27061 agcgcgccct cgtcaccggc ggcgcgggtt ttctggggtc gcacctctgc gaccacctcc
    27121 tccggcgggg cgtctccgtc atctgcgtcg acaatttcct gacgggcaac cccgacaaca
    27181 tcgcccacct caccggacac ccccgcttcc gtttcatccg ccacgacgtc acccaggacc
    27241 tctatctcca cgaacccatc gacgcggtgt tgcatttcgc cagcccggcc tccccgcgcg
    27301 actacctgga acatcccatc aaaaccctca aggtcggcgc gctcggcacg cacaaaatgc
    27361 tggggttggc caagacgaag aaatcccgat tcctgctcgc ctccacctcc gaggtctacg
    27421 gcgacccgct tgtccatccg caacaggaaa gctattgggg caacgtcaat ccggtcggcc
    27481 cgcgcggggt gtacgacgaa gccaagcggt atgccgaagc cctctgcatg gcttaccacc
    27541 gcgtgcacgg cgtgcgcgtg cgcatcgcgc gcatcttcaa tacctacggc ccccgcatgc
    27601 agaaacacga cggccgcgcc atccccgaat tcgtcatggc ggccctgcgg ggcaaatccc
    27661 tgccggtgtt cggcgagggc ctccaaacgc gctccttctg ctacatcgac gacgaggccg
    27721 aagggctcct gcgcctcctt tattccgact gccccggacc ggtcaacatc ggcaaccccg
    27781 ccgagatcac catcctggac atcgcccgca tgattctcaa actcaccgga aaccgcggcc
    27841 gcatcgtgca caaaccgctc ccggtggacg accccaaggt ccgccaaccg gacatccgcc
    27901 aggcgaaacg cgttctgggc tggaccccgc gcgtgcctct tgaaacgggc ctcaagaaca
    27961 ccatcgcctg ggtcaaaaaa aattatctgc cgcgcggcgc gcggcgttga accgacgcgg
    28021 gaaaacctct taaagaacgg agcggcccca tgaaaaaaat ccgaatcgtc tccctggccc
    28081 tggccctggc ggtgtccttc gccgtcctgg tcgtcttcgt ccgcgggttg ttccaagggc
    28141 tggaaagcca gtccatcgat ttccggttca agatccgagg cgaccgcgtc ccgaacgaga
    28201 acgtggtgat catcaccgcc gacgagaaat ccatcgccga gctgggccga tggccctggt
    28261 cgcgcgccac ccacggccgc ctcatcaagt ggctcaccga cgccggcgcc aaggccatcg
    28321 ccttcgatat ccttttcacc gaacccgaca acgaacaccc ccaggacgac gtcgccctcg
    28381 gccgcctggc gtccaaaagc gggcgggtcg tcttcggcat gttcttccaa aaaggagagg
    28441 acaacctccc cgccgcgccg ctcctgccca tcccgccgct gcggaacgac cgcatccaca
    28501 tgggctcggt gaacttctgg cccgaagtgg acggcgtgac gcgcaaactg ccggtctgga
    28561 tggaatacga cgggcagttc tatccgtccc tgtcgctgtc ggtgctggcc gtggcggaac
    28621 aaaaagaccc gcagaccctc ttccaggagc tgaacctgcc cacggaagac ctctggaacg
    28681 agttctccct caacttcgcc ggcggtttcc agatgttccc ctatttctcc tacgcggacg
    28741 tcctgaaagg ccgtgtctcg cccgccgttt tcaaagacaa gatcgttttc gtcggcggga
    28801 ccgccaccgc cctgttcgat ttcaaagccg ttcccaacgt gcccctcttc cccggcctgg
    28861 agatccacgc cgtggcggtg gacaactacc tgaacggcaa ccacctgcgc acgatcaatc
    28921 cgctctggac catcctgctc atcctcgtct tcggcctggg atgcggactt ctgttgtcgc
    28981 gcgtgccggc ctgggcgggc gctctctcca cggccgcctg gatcttcggt tattattcgg
    29041 tttgtcaggt gcttttctca aaaaaacaca tcgtcctgga tttcgtggcc ccggccaccg
    29101 tggtcctcgg cacctacgtg gtggtcctct tctaccgttt cctgacggaa cagcgggaaa
    29161 aacgctggat caaggggacg ttctcccaat acctgtcccc caaggtcatc gaggtcatca
    29221 cctccgaccc cgggcggctc cggctcggcg gtgaagagcg cgagatgacg gtcttcttct
    29281 cggacttggc cgggttcacc tccatctcgg aagccatgaa gccgcccgac ctcgtggcgg
    29341 tgctgaacga atacctgacg gccatgagcg agatcatcct gaaatacgac ggggtggtgg
    29401 acaagtatat cggcgacgcc atcatggcgt tctggaacgc cccggtggac cagccgcgcc
    29461 acgccgtact ggcctgcctc acggcgctcg aacagatgga acggctggcc gtccttcagc
    29521 gggggttcgc cgaacggaaa ttgcccgcca tcgactgccg catcggcatc aacacgggga
    29581 acatggtcgt cggcaacatg ggctcgaaga accgcttcga ctacaccgtc atgggggact
    29641 ccgtcaacct cgcctcccgc ctggaggggg ccaacaagcc ctatcacacg cgcatcatga
    29701 tctccgagtt cacctacgag aaagcgaaga acgacatcga ggtgcgccag ctggacctcc
    29761 tgcgcgtgaa aggcaaggcc attcccatca aggtctatga gctgatcgcg gccaaaggcc
    29821 gcctgacgga caaccagaaa aaggccttcg cctcctatga ggaagggctg gcgttctatc
    29881 tggaccggaa atttgagaaa gcggccgcgc tgttcaaaaa aacacaggcc ctccttcccc
    29941 acgacggtcc ctcggacgtc tacctgaacc gctgcgccgg ctacctggcc gcgccgccgc
    30001 cgaaagattg ggacggcgtg tacgtgatga cgacgaaata gggcgggccc ggggctttct
    30061 tctcaccgat ctatggaagt tttctggcga ttgctgttcg gacatctcct cggagatttc
    30121 actctccaga ccaactacgt ggcggcctgg aaacggcgcg ggccggcggg gcttcttttc
    30181 catgtgggca cgcacatcgt cctttatgtc ttcctggctt ggccgtatct ggggacgacg
    30241 tggatacaca tcggcccctg ggcgctgaac ggctgggccg gcctgggtct catcttcgtg
    30301 tcgcatgtct tcgaggacca gtggcgcatc tggtccgtga cggcccagcg ggcgccggac
    30361 aacctgcttt tttatatttg ggatcaggtc atccacatcg tgatcctgtt cgccctggcc
    30421 cccgtcacct cggacaggat cattgaaaat acgtggcccg tcatgggctg cctggtggtg
    30481 gcggcgaccc atttcaccac ggtctccgtt ttcttcatcg aaaaggacct cttcaacatg
    30541 aagttcccgc cgacccggga aaaatacggc gccatggccc agcggttcct cgttttcctc
    30601 tgccttcttc ttcccagcgc ctgggcgtgg ctggtgctcg gcgccgtcgg cctgcaggtc
    30661 ctcgttcggc ggcagatccc gtcgcaggcg gtttcaaaga cgggattggt cttgggcaac
    30721 ggcctggccc tgttggcggg tctcctggcg agacggcttt acttcgcgtg acgacgcgcg
    30781 cgccgggcgt cgcggaaaaa tttttatata ctttatgccc agcttcgcgg cattctaaaa
    30841 aggagccctc tcatggcgaa actcgataaa aaacttctgg cggcccaggc gctgagcgcg
    30901 cgcccccgcc ccgcggcgaa accgaccgat ccggtgctgg tgatcgttcc ccatgtcccc
    30961 gttccggacc gcatcccgga agaccgcgcc gcggacaacc tgatcaagct cctccgggaa
    31021 catttccgcg tcgtcatcgc ggcggaccgc cccgcccccc tggaagaccc ctaccggcgc
    31081 catttcctca tggagggcat cgagatcgtc ggcgggcaac aggacttccc ccaagagcag
    31141 cccg
//
LOCUS       DAOTFD010000010        28390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1089_length_28390_cov_4.803000, whole genome shotgun sequence.
ACCESSION   DAOTFD010000010 DAOTFD010000000
VERSION     DAOTFD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 28390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28390
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_28390_cov_4.803000"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /gene="ispG"
                     /locus_tag="P5079_01400"
     CDS             complement(<1..1167)
                     /gene="ispG"
                     /locus_tag="P5079_01400"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRY28673.1"
                     /translation="MSSSPAEALLKQLPFSPSLFNYRRRPTRTVMVGNVGVGGNNPLR
                     LQSMTTTRAGELEATVAQIERLVQAGCEIVRLTVPNEEEARQLQKLREGLDSRRLAVP
                     LVADIHFRPEAALAAAEYVEKVRINPGNYADVKLFKTREYTPAQYEEELARVEEKFSP
                     LVLKLKRLKRALRIGTNHGSLSDRILNRYGDTPEGMVESALEFVRVCRKLDFHDVILS
                     MKSSNPKVTIAAYRLLAARMTQEGMDYPFHLGVTEAGFGTDGRIRSAIGIGGLLEDGI
                     GDTLRVSLTEEPELEIPVGRRIASRYERRTAVPPSVPSPDETLSPVDDFYHYRRRPAR
                     EVPVGPFRIGGTQTIRVALGLPKKNSDPRRRMDSLEKSLRGSPDQPPEMLEWRIS"
     gene            complement(1337..1528)
                     /locus_tag="P5079_01405"
     CDS             complement(1337..1528)
                     /locus_tag="P5079_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28674.1"
                     /translation="MFTIGEFKGNPTITLKQSEEDAYGFTFGLTKAKLILDHFEDIKR
                     FYEQNLNKAAAGKKPTPGK"
     gene            complement(1682..3028)
                     /locus_tag="P5079_01410"
     CDS             complement(1682..3028)
                     /locus_tag="P5079_01410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY28675.1"
                     /translation="MSHDPATPRKNLLFVADPLPFPSSFPLDAFFSSLAARWDLLVLS
                     TDKPGFSLPAGATFSVIKENAFAEEARRVFSTKPLEAVIFLGADAVKRRFFPVRQVAG
                     RDQTLVCFFPETHALHRLPRGGDGLSQKRRWREILSEADGLWAARPTDRDRILRTIDP
                     RLDVEVLPPAREAPALRDWAGQRLEILSADTAAKNAYGTPADLTSIVLLTHNDGDYLK
                     QCLARLRRHTRGPYEIIVVDNATTDGSSAFLEKEKDVRLIRSPKNLFFSGGNNLGLRA
                     ARGNHLLLLNADTAVTPHWLERLVLRAKRDKTAGLLGPYTNRAAGQQGVQKPGYSSLR
                     ALDAFSTRWARRHEGGRLEVPRLDGFCLLIKREVMKAIGLLDERFGPGGYEDYDYCLR
                     ARQAGYKLLLVEDAFVHHHGGKGYRDQDYDAARERNRGLFVEKWCQQSLAFLDEVW"
     gene            complement(3059..3991)
                     /gene="trxB"
                     /locus_tag="P5079_01415"
     CDS             complement(3059..3991)
                     /gene="trxB"
                     /locus_tag="P5079_01415"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRY28676.1"
                     /translation="MDIRNTLIIGSGPAGYTAAVYAARAGLKPLVLGGFLMGGQSGGQ
                     LMTTTEVENFPGFPEGVPGPELMDRLRRQAARFDAEILDKDVVKADLSRPPFRLTTDD
                     AEYLAKTVILATGATARRLNTEAETRFWNKGISACATCDGALPLFRNKPIAVLGGGDS
                     AMEETLFLTRFGSQVILLHRRNEFRASQIMLDRVRRHPKVSIKTPYVLEDALGENLLE
                     KIRVKNLATGAVEELAANGLFLAIGHEPNTSLFKGQIELDEAGYVKTDGHARTNIPGV
                     FAAGDCVDHTYRQAVTAAGMGCMAAIQAERYLEA"
     gene            complement(3996..4673)
                     /locus_tag="P5079_01420"
     CDS             complement(3996..4673)
                     /locus_tag="P5079_01420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY28677.1"
                     /translation="MPTHIVLIRHGETDWNRLQRIQGHQNVPLSPLGEQQAGQVGRRL
                     RQLDIAAAYASDLDRAARTADIALADWNGRVVHTDSLRERCFGEWEGRLWPEIEKTFP
                     TDARRFHEDPVGFSPPGGESWNQMQERVFNKIEGIARDHPDQTVAVFTSGGPCKAAIL
                     AALGLPAGQWRLWVAGNASLHRLWHAGANGNGTKPWRLLSFNDVAHLEPGRIFSDPSA
                     NLKEKVE"
     gene            complement(4931..28339)
                     /gene="nagB"
                     /locus_tag="P5079_01425"
     CDS             complement(4931..28339)
                     /gene="nagB"
                     /locus_tag="P5079_01425"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRY28678.1"
                     /translation="MATFLFGMGRLLGEDAGAAFLKSEKAWGEEFSEFLRRTEWVVLE
                     RDGKRFVAGTVQMAGAAKPFLLGLSANEDLTVVSSDEAGLREWMRRAPLENAADVADR
                     LARLWPFLDAVDLDRSAGAARPSPLTLSTQKIQDALAESQQELGVLLKNDAAGQKARR
                     RFLGTVGQWLTARFRTVFSLRRLLRNAPTQGTTLKKEPSPPTVVRLDSFDAMSRWTAE
                     RIAERIRQKPDLVLGLATGSTPLGVYKELIRLHRETGLDFSRVRVFHLDEYVGLPGTH
                     DQSYQYYLRENFLRHVNVAEEHIRYLNGKAPDLPAEARRYEQAIREAGGIDIQILGIG
                     RNGHIAFNEPGAAFDSRTRVVDLTAETIEDNSRFFKDKQDVPRRALSQGMATILAAKT
                     IFLLANGEGKAPAVARAAEEKPSVSLPASVLQYHPDTTFVLDRPAASELQNKSPPKVV
                     RRWLRQILPSLTVDDALSWKNLLRAAGVIAILTGTFSLLLTQFSVGDIAETLRLPALV
                     PLASRWALPAISQGSFVYLARTFLYSIVLLTLVPGTSFWISHLIVTRVARKFAGGREL
                     SSFQKKTLHLLVPFLLIYLLLAPLFNAATRRAGAPSEGLVASAQTSPENAAVARNALS
                     VLLPRHTDGLSSVVLGPWLSPDIKDDVAGRYVSLLDQILLNTLNGVDPSVVVHEVGHH
                     YYLHGLTDEQRAYWKNLFLQSTDAIWDQTVGLPQDGEWEDFSYTFGQFVKNRALLLNR
                     TFYSSLLRRKVLFVAEQFVFEKDGRLYLRLHAARGNRLTFRDVPLETDLRPGTSAFRE
                     FLWNTLFSNTPGLRRPDAVGPMKRFPAAFKTFDWLAEKTALLKYGLSSLLAGFLALLA
                     GRRGRMGRTPQEESRRTDLLSDRNLVALADRLMKHRTSADLAPEDARLLEALGVGERN
                     IREFFKPLPTNPTFDWSLERTETRVNSMDAAFERFRANPRFSHFSPEDWRKIKSRLDA
                     LKTIEPHLQELAPGREIVSVIVHGSYLTDPKATPNDIDLVLLVKGDGRPSETRRRLPG
                     ADGLPTADVSVLPLDSLLPEKRTGSLAAALLRIARWGLTLHGEPLIESLPPAPGLLKI
                     YAAEKLLENVSDLWFNEALTTNTARRRAAQVLSLLAEAGLPPTLNTADLDKIEVNALL
                     DGLYMEILRAKDLLLRQHIVETLISARDARPAVVQEAGRETRPALTPAPRDRFKWTPR
                     INYNEPLSLNKHRKILISVRDTFGGEPLMWQVHKRIEATNALGKGKRGPPALKFQTAL
                     SFLMLVSRNLYLPLGQRQALLKKLLAVPLDGDTNAWITQELCPALEAHLKKWGVPLKA
                     RPKPQDILLRSLLGISFQDRYYAPSEAETARVRSLLRRQELKPDDLTQHLLALLYANT
                     LRFYPKEYLGLIPKNLVSRHDVDIEDEEYMWVGGAFRGGYKMDDAQLVGSVFAVNSEL
                     GHMFDWLIRKKHFRTYIGELHVIDRHTATRDYEGYNAEVFDRFMVQVGKKEIPWLTFD
                     RTGDYVARLVDLAREALSTPDADKVFKDVFYTVFSTDRQSDFLKRFAAGRGDGVFADM
                     TVSEKYYFGRLAAEYYQEETRGLAAAAALAGFRESLERDGVSAEEFRKEADQLIGIFG
                     LSTHARPFLDDPVMHGYDFYTDEIHKLAERISSDFILHLTLTMHRAGVPMKVQPYLLA
                     KAIEHIDRRYPDDTGRIAEKIEPVMPVLRLIESIDDPLLRQWLAELEKDGIVAKGEAL
                     VSETKSVSAVYGGFKTIGRLAKSMANASVTAVTWGAAVASLGVLLINSLWGLLGATVA
                     LPHLTQAALIVLAAHDVLPDRGLIPEWQDAAPLTPAAVADPSSFSGKIRTSLNKYRVA
                     ELRDGQLTVNHALMARLPHGMQRWIYRHEVNHLKFEHRHPTYSATAIGRWAEEFWVST
                     LDFPALVRSPKWDLPFARGHYLTGDLPTLLKNFWETHTDIIHTLGIASLPDFVHSPWD
                     ALSRFFSSRAVEPSEEHRAILADLNDTFLGENGLWLENIDAPGLSALMNSEVPVQVDL
                     RIADTPELIVSHDLHLLGNTIRAGPDNTFAVTLQSALFEMAPAVLKIVLKHELKEISL
                     IAKGVPPEEAHRRALAEMTADDQKVLTFEVSQWIRWKKSQEPGSQADRNAARWIKRVQ
                     GQGQAVVPIQETEHGPALNAVLEEVHQALDHVPQTGNILVTSATLGDDIRIFGTTIVI
                     SAAALADPARREDILWRVLLKSEKSVDAGEIQAALRTLAALKEPLPTDAGGLLLWFLE
                     ALVKARNDSPRDTPPLLSLLDGFIYHVDRILDSRYYRSDFAELLLELSDLLAWEPENP
                     FLRNTKNSYLLPSLLEAFRDPANHGGFWGEVNGLHTVVFKRRTALAGFGVQGVFTGVN
                     WEILGAKPAPNGHSGIKEFDAINRSSMFEFKYLGSLEDIYGQFAGIGLREDGHAHNRL
                     LCHLCFLAQQFIYGTLEGFGKPFSNPAIKRRLSGLRNFVIHMQHANRRLTQALYDFAE
                     SRNIPMQTTGEGDLALKLSAAQVADFLTDPKTIELYALEAMHGNHPEAAAAAAKLQEA
                     LAGPKGQILLKTLIAVDNRIRRETKNKRQFDFIVSIRHPAIRQVAAKKARADADFNPL
                     ITEKPAVLAPGEEDRLQRTDKALEAAIREAVASLLPRQDFDVISRGSTARLTHTPGSN
                     PDHDLCVRVPREWTPSDLTDFLSKNHARLQKVLRETLTKQLGELNLYPRRLRFEVGQK
                     NAVAADPLSLFATLSNVQTDQADEGVYMLPIQIYDEDHKPVVEMDITFTNSDKYANPY
                     PEYFAVQMAHVVRLGGQAAADRLLQDIRFAKKFFKEVVRAYKHWEKKRSPLSSGGPTG
                     VGVEQLIMQSGKVSDDDKGQTILEIGGFDKFLERVAAIDRDENGNIRSKEERLALWLV
                     HNPFLEPANFIDLMWESVWIRLALAARRYQEAKRNGEVLTLADLATAAAARTSHPVSQ
                     SKPGKPARGRPSRPAREDKTLAVRLVSDNKLNRVNTLTKRAVTALQKQKLPVLSGYAK
                     VQVVFDGKRKTTVYVVNLSLRKEADEAAAEKAFRAVLGGLDPTADFNNVPAEVVPPTP
                     PKETVRESSKPNVAPFALVVRADISKKAFAKARGQMIQRLGIDKDAVAVERHSESRAV
                     TLILRAASAEMRNRVSKEAFLHLNAANPKIKLIQMDAPIQPSAAEEAEVADEGEERAD
                     QSVPVQTPPAGLVPPAAVRNAPLLSATKPNNAGPLARAAATAVEHLAQTIPAAGPPTT
                     AVVTAGQRVLERTSPRAPPVHDSSDLEGVHFIGKAAKLVKHVFNLIDWLKQHDVNVEE
                     SLGVMWDTYEAIWAKMHILNAATHSNVSKILSSNSHLSMTLTWLDGVYTSDDQPPNPD
                     RNVDVPGAQRVTLVHTIQAYFHKSPNPESEIVEGKRRLAGEKGELRQFLETIKPGGRA
                     EKAMGALDEIILGFDARGYRELDRFVEEEVDRQCAQMGIRRERIKIVHVDDAARPRTH
                     QDMIRSINELTDLYGEETVKAILEGVTYSRLVGLDLELLAQQMFRTAGYWILEAGSEV
                     LTPEGMYVTELDTVVAKAYWQVEGQWRLSPINMAGGSGTLDIDGESVPIRSEIVDGEQ
                     QVFVEVKGRRVPIRIKVISSEEKSARTPLKPEEIFSSKVGYKMNYYKKHLAAIEDSVS
                     RGLGFPIKLDGFLFFMDIGTAVPTRKNFEREQNRLTRLKEYLVQQEPGMTETYRLPAK
                     IVFVDDLTDMGPVDDWLETVAIDDAVRAASRLADAIAKVKSEPGDGADRRKAFLKSLV
                     QWGWKWETALAGRFDEASLLKLDTAEEQIGFLVPLAAEFDLHSGQLADLAGLLWDTPP
                     APAQINQAVPAERRLVSAPSTKPAPAATPIAQKAVSAVETMAQFLPGSEATKKVEAAA
                     AEAVAPKTAGADFLTMAADGTGRLTLEMLKKFVMGLGPEERGLEYLLAQGVITLPAEW
                     EAAWRGFSQSKKRALPTGAKVATGLFRRDAIGRMSVEVSEQGAQATRPDEKKKTTKLF
                     VPPALGQGILSNTLVEVVYRESAAKEARGRSVKAQKGKKGKQGKKEKAGSQGGKRGQS
                     QPEREIVSVRPVGGYPMDMMLGRVLKRGNKLVLAPLFRPGGKSKRVYDDLDLEITEVA
                     GRPMEDQDIVQAFVKPHGSGFIATPIILRGKKITPEIAKLEIGLRAGARGYFDAEVVK
                     QSEEVGRSIESERSPEKDFERLRKELAGNPSGLRIEDMMDKAFITIDPEGAGDLDDAL
                     HVEKHADGSYTWYLATADVGNYVLPGTPAFRTAAKIGNTFYMHIADGLGESPMNHPVI
                     SKYVASLLAGKASLAMITKMEFDAEGNEIKSDVFMGLVGVQGRYTYDQVTELWKGQTD
                     TGITHLDQVALSRELASKLTAKDQERGKMDLEFPESGLRKMGPNDWRIVVEEQGPLLK
                     ESHRLIEELKVYGNQGLAKILTRITKEYGVPHLSRVHPEQDEEKEDKIREDLQRIGAP
                     WRKGQTISEYLAALKAREDLSWWQKKALSLKILKSRMSARYDVIDQEGHQGLALEAGE
                     YDHPSTPIRRFSDMYNQALLNAYLTGQDPRKIYEAILADLRAMGFADLKKYVEHLNAR
                     ERAAKAMENELASFGTIWELSKPEYAGQRLTGYVIDSTPGTNGRPPRALVQLENPMVV
                     IEVSGDQAEGLDIFNEVTVKINKGQVEDFQLNVEINPPSTKYKSALNALAVASVWLGV
                     ASLLGHALGVWAFLPGAIVASRFLLQFGLIFAAAHEWLPDYGLIPEWRNASPLLPPTP
                     RNAARPTPKDRWMRFLDRYRVAGTQMGFVSVRAEIMRRLPPFLQRAIYRHELSHIRFQ
                     REHLSQNSSRPLLGRWIEEFLVSSREWLPRAGLDSAAPLLTEVYGETGRQNPEIIRWL
                     RTIHLSRELMQDIRILLRQSPDAWDFEAIHRLIEIFQDPRVDDTLVDHIKRLAPVAGL
                     SRVIRSMDMGVFDVPANTVEWDESTLKSLRTFLSAAELLADAARSAAAGHVPERLAQW
                     ISYREEDTAKPLELDAVLTDAADPRRLRILTEVPGHPEAQTNASDTLIFIQKKLDALT
                     AALTDTSTDGNRLGAAEKVDLVLSLRRNTAYGVGFKNALDSLLEGYRQKWASLGRDVS
                     VQRLFVAKGNFFHPQAARIHAHRRYAYSNQDYLRRRMNHLRLQHGLLRGVRLIATDID
                     NTLAPHNGAITPGNETYLREALALGLPLVFITGKSDMDHLLQQQIGGFISRLSPQHRQ
                     NVFLFTGSGSRFYRFDEQGAPVKEWEHPVLSDPRTRTAVRTVVSEVFEKAAKAFHLDQ
                     ARHKPFHDGDVLDPGPGQAAQQAPLALFIEDALRNDPRLSAWGVQLNVTVGGNGDIGV
                     DPSGKGAAMGRVMERFGLKPDQIFFSGDSFFKFGNDNPVRREHQNIGYAHVKEPKETA
                     RYLETLVDKIRTGVDRDIRKQYTYRSQHAHRAVALSSLLVWVAPVLALAAALWMGSEN
                     IVWVLPTASFFGLGTLIHAVVGTTVDAVHGLDDTAPVDPRIEQLIRTWTTERSALPLE
                     VVYQTDEEMEKRFGNNASLVVVSAKNKVYIARRWAAATRNPVVRLFRPIVLAVLLNDR
                     LKDPKRTFRFSHYFWKMPASVPVVLWLSVVFLYRNSLKPAAPSLWARHELYQPVTDPA
                     QMETPDISDNFLGKLEYDLAQWRKNGSDPAQRPVVYMRNTGGGGDVLNNIYLAEKYRA
                     MGAEVVLISVHQKKAGLKVPRDKFVKEENATEEDPLWKWDPAQKRYVYDDDAGYGRGL
                     AETEFIEPVNGQVFRVTRRTRIKGADIPIAEANLVEFMRDNYGADNFFMIGMKDSPEE
                     VAQAEYDLFQKRFAGRNVYVVITGCGGDFISNESEWKCSPVNEEFHMLVLRQMLKRYS
                     KTRKKENFRWRSMVFQESSPGADLESTPAGMQKVLDDLWSQRILIGTMTPWENQEGVR
                     KAFEAVALGIASSANLRFINKIGPFFSAGNLNVSVLVRSVVRGDGLAELLLQKLGATA
                     KDLPDLARKKGFEGKLLKALNALIQMPDLQAHLPAGLSLPSGTRQMLEKARAGTLSRF
                     DTYVLNRILLQSIYPRGTRPGRKTGWLQPLMTGLGRLLPKRVLSLLPLPGYFRLRGKL
                     IVEGTMQRDDTYTFLGKDIQIPGGSVTRDSLFFHWSRTRDWLVKNTGLPPPSVAKHLP
                     FKELAVQIESTQGSTREGIKPTEAEFDVLALYRALRTPDRAPLMTRILTGRTAGISPQ
                     IIRNFFAEFEPMVFNILGHSLDLRLQIMGDNAQETEEVRAVILHIAKGLLDRSDLPYE
                     LTRKAATLLKLNPLRDPAYSTYIFPRRERLLNVDRAIAFAQQIYQWRDARPLADRLRA
                     ASTAQEVLTEALDFLLNPHNNPFIHRNIQFNVFDLADRAQKELTYALPGLSGPQHELV
                     DAALNELNRMLKLRQTPKSLSQRLGKGLFAEALWQIARRYEKTELHRHISGSLSPLLL
                     MVLYWRNPKARQQFLREVLHQNPSAVPDIDAFFAEHFGGPDSPADRDALSRWLSSLPQ
                     DDIRLTNPGFVASLQSFFGSDEKLQAALKKAKNFIEYRPKPKKGSLKTYVEFLATGEI
                     VLRNDPAANRVAFRESVLEEFVRDNVTLMELQVSGLRPGEQGSLEDVVSNYMAAFDDV
                     EDSSAGHAKVSAIVVARKEPAAVLTKDAVDAAQRAGGEFAASRERAGLSTEEFLDELA
                     AWLENRVIDPALSSEEKIEERRWLGDFIEQLRWHQKKRGLTNAALAELVFLLEATRRE
                     QTRRAQDVIDLKKRWATERPHDAERIVGIGSVGWEGDFFPWTLLPAMRLAKKNGLKNS
                     IHVGEAWAEGEEMPAMLRVKQIVTSGHLDRLEHGTILGVIPRDFTQPPSAEELGQFRR
                     LQRDLLTFLAQTQIAVVTMPTSNVNLSIFGNYARHLLQFFFKQALRVAVAQDNSWIFH
                     TRGSPGERVKVWLAHPDMTFAEWLAIEQNGFENAFTPNNLQGLDPILEWTEPNTPTEA
                     PPAAVRPALPAPAPSLKSFLAQRPRLSRVLSLIGFNFLRRMLSRLLQAARAFGEFLND
                     VRLRPGGDVSPLSRSLPRSLRQAYAAGETDQYLRPLVLVDDQDRPMGRVTGKDVLLSF
                     NARADGPRQLLTPFARPASEEFSPFHRAAGATPDVFSLTEVPNVPQSSPLLPRPTASE
                     TLGSAAVANGIKTAVVVSEKHAPRIVSALRGNHAAEPAETVRLLSAEASPQETAETVA
                     REMRTSPLVIAHFGGVAEAAGRGDAAAVRQNAEQLDSSLEKVLAQARRENAVVLVTSD
                     RGLAEHLGAGANPLPPLSTKNPVPFHFYDPALLPQDPRSSVLLRTNGSLADVAPTALQ
                     ALGIQPPADMTGRSLLPENYFAGRTPRKVVLLLVEGLGENPDLRDPADPLRSARLPIL
                     RSLAENYPKTLLAAQGQELGLSPEEPADDSTGFFAIAAGRAPPNAFERLRLDPTNKVL
                     TEHSNFLQALRQLKSRGDALHLFGTLSPSEIHSSLEHVERLLLLAKAQGIENVYLHVV
                     LDGVDAPPRAGLRALKALEKLTARAGVGKIVTVMGREYGMNDSGGWSKTAAAYQALVE
                     DVPPLPSDPKTDVPSDRPTATSTEKETPVPTGTSEPKATPTLPIPAQITPCPPVHWAP
                     SNVELVMADLRGWVEIRDGKLFIKNVGRQALLEQVERGRSLKSKGTRLMFALLVDFSG
                     QDLSAAQRELLIQRLARELGIPETMMEPDLLFDAQRWPTADRIAEHIQKVSGSGGWRA
                     WKVWILADALAAPWWEKVARRMGWPTVAFKLLDVGVRIEVAGAALDWLQALLRERYHY
                     TDDEISRLLAPYREGRSVVLPAEKVGMPSLEKLRDEQQLFDIQA"
BASE COUNT         4700 a         8265 c         9688 g         5737 t
ORIGIN      
        1 cgaaatgcgc cattccagca tttccggcgg ctggtccggg ctgccccgga gggatttttc
       61 gagggaatcc atccgccggc ggggatcgga attttttttc ggcaggccca gcgccacgcg
      121 gatggtttgt gtcccgccga tgcgaaacgg ccccaccggg acttcgcggg cgggccgtcg
      181 gcggtaatga tagaaatcgt ccacgggcga aagggtttcg tccggcgaag ggacggaggg
      241 aggaacggcg gtccgccgct cgtagcggga ggcgattcgg cggcccaccg ggatttccaa
      301 ctcgggttcc tccgtgaggg acacgcggag ggtgtctccg atgccgtctt ccaggagtcc
      361 gccgatgccg atggccgagc ggatgcggcc gtcggttccg aatccggctt ccgtcacgcc
      421 gagatggaag gggtaatcca tcccttcctg ggtcattcgc gccgccagga gtcggtaggc
      481 ggcgatcgtg accttggggt tggatgattt catggagagg atgacgtcgt ggaaatcgag
      541 tttgcggcac acgcgcacga attccagcgc cgattccacc atcccttccg gcgtgtcgcc
      601 gtagcggttc aagatgcggt ccgacaggga accgtggttg gttccgatgc gcagggcccg
      661 tttcaggcgt ttgagtttca ggaccagggg ggagaacttt tcctcgacgc gggccagttc
      721 ctcctcgtat tgggccgggg tgtattcccg agtcttgaag agtttgacgt cggcgtagtt
      781 cccggggttg atgcggactt tttcaacgta ttccgcggcc gccaaggcgg cttccggacg
      841 gaaatggata tcggcgacaa gcggaacagc gaggcggcgg gaatccagcc cctcgcggag
      901 tttttgaagc tggcgcgcct cctcttcgtt gggcaccgtg aggcgcacaa tttcgcagcc
      961 ggcttggacg agccgttcga tctgggcgac cgtggcttca agctcgccgg cgcgggtggt
     1021 cgtcatggat tggaggcgga ggggattgtt cccgcccacg ccgacgttcc ccaccatgac
     1081 cgtgcgggtg gggcgccgac ggtaattaaa aaggcttggg gaaaaaggga gctgttttaa
     1141 gagagcttcg gcgggggacg acgacatatt tcttattata ccagttcgat acaggttggc
     1201 cgagcggcgt tcgtgtcgga ccacgccccg ggctggcccg catgcgaaac gtcccccggc
     1261 ttcaaagaac gtcggaagac aacgccggag gggcctccgg cggaaggggg tggggaacgg
     1321 aagactttgt tgcggtctat tttccggggg tgggcttttt tccggcagct gccttgttca
     1381 ggttctgctc gtagaaacgt ttgatgtctt cgaagtggtc gagaatgagt ttggctttcg
     1441 tcaggccgaa ggtgaacccg taggcgtctt cctcgctttg tttcagtgtg atggtggggt
     1501 tccctttgaa ttcgccgatg gtgaacatgg gatttaggct ccttgagtgt gattgacggt
     1561 ggccaaagtt ccttttccac cgtcatcgtc cctggccttt aaccaggaac gatgggcgag
     1621 ccgccggctc gtcccttgac ggtccccgcc gggtcggcgg gggaacatct ggagatactt
     1681 tttaccatac ctcgtcgaga aaagccagcg actgttggca ccatttctcg acaaagagtc
     1741 cgcggttcct ctcccgggcg gcatcgtaat cctggtcccg gtatcctttg ccaccgtgat
     1801 gatggacgaa cgcgtcttcc accagcagga gtttgtagcc ggcctgccgg gcccgcaggc
     1861 aataatcgta atcctcgtaa ccgccggggc cgaagcgttc atccagcagg ccgatcgctt
     1921 tcatgacctc gcgtttgatc aagagacaga acccgtccag gcggggaacc tcaagccggc
     1981 cgccttcgtg tcggcgggcc cagcgcgtgg agaaggcgtc cagggcgcgg agagaggaat
     2041 agccgggttt ttgaacgcct tgttggccgg cggcgcggtt ggtgtagggc cccaggagcc
     2101 cggcggtttt gtcgcgtttg gcgcgaagga cgaggcgttc cagccaatgg ggggtcacgg
     2161 cggtgtccgc gttgagcagg agcagatggt tccctcttgc ggcgcggagg ccgaggttgt
     2221 tgccgccgga gaagaagagg ttcttggggg accggatcag ccggacatcc ttttcttttt
     2281 ccaggaacgc ggaagagccg tcggtggtgg cgttgtccac cacgatgatc tcataggggc
     2341 cccgggtatg ccggcgcagg cgggcgagac attgtttcag ataatcgccg tcgttgtggg
     2401 tcagcaggac gatgctcgtc aggtccgcgg gcgtcccgta ggcgtttttc gcggcggtgt
     2461 cggcggagag gatctccagc cgttgcccgg cccagtcccg taacgccggg gcttcccgcg
     2521 ccggcggaag cacctccacg tccagccggg gatcgatcgt ccgaaggatc cggtcgcggt
     2581 ccgtcgggcg ggccgcccag agcccgtcgg cttccgaaag gatctcccgc cagcgacgtt
     2641 tttgggaaag gccgtctccg ccgcgcggca gccgatggag ggcgtgcgtt tcggggaaga
     2701 agcagacgag cgtttggtcc cgtccggcga cctggcgcac ggggaagaac cggcgtttaa
     2761 cggcgtcggc gccgagaaaa atgacggcct ccaggggttt tgtggaaaag acgcgtcggg
     2821 cctcttccgc gaaagcgttt tcttttatca ccgaaaaagt cgcgccggcg ggaagggaaa
     2881 atcccggttt atccgtcgag agaacgagga ggtcccaccg ggccgcgagc gacgaaaaga
     2941 aggcgtccag cgggaaggag gagggaaagg ggagcgggtc ggcgacgaaa agaagatttt
     3001 tgcggggcgt tgccgggtcg tgggacatcg tcagcgaggc ggaacgggcg tgggccgctc
     3061 aagcttccaa gtaacgttcc gcctggatgg ccgccatgca gcccattccg gccgcggtga
     3121 cggcctgccg ataggtgtgg tccacgcagt ctccggcggc aaagacgccg gggatattcg
     3181 tccgggcgtg gccgtcggtt ttgacgtacc cggcctcgtc gagttcgatc tgacctttga
     3241 agagggaggt gttcggttcg tggccgatgg ccaggaacag gccgttggcg gcgagttcct
     3301 ccacggcgcc cgtggcgagg ttctttaccc gtattttttc caggaggttc tcgccgaggg
     3361 cgtcctcgag gacgtacggc gtcttgatgg agactttggg atgacggcgc acccggtcca
     3421 gcatgatctg ggaggcgcga aattcgttcc ggcgatggag aaggatgacc tgggaaccga
     3481 accgcgtgag gaagagggtt tcttccatgg cgctgtctcc tccgcccaag acggcgatgg
     3541 gtttgttccg gaagaggggg agggcgccgt cgcaggtggc gcaggcggag atccctttgt
     3601 tccagaaacg cgtttcggcc tcggtgttga gccgccgcgc cgtggcgccg gtggccagga
     3661 tgaccgtttt tgcgagatat tcggcgtcgt cggtggtcag acgaaacggc ggccgggaca
     3721 agtccgcctt gacgacatct ttgtccagaa tctcggcgtc gaaccgggcc gcctgccggc
     3781 gcagacggtc catcagttcg gggccgggga cgccctccgg gaaccccgga aaattttcca
     3841 cctcggtggt ggtcatcagc tggccgcccg attggccgcc catcaggaat ccgccgagga
     3901 cgagcggttt cagtccggcc cgcgcggcgt acacggcggc ggtgtagccc gccggcccgg
     3961 agccgatgat gagggtgttt cgaatgtcca tggggtcatt ccaccttttc tttcaggttg
     4021 gccgaagggt ccgaaaaaat ccgacccggt tccaaatggg ccacgtcgtt gaagctcaac
     4081 aaccgccacg gtttcgttcc gttgccgttg gcgccggcgt gccacaggcg atggagggag
     4141 gcgttgccgg ccacccacag ccgccactgt ccggccggca ggccgagcgc cgccaggatc
     4201 gccgctttgc acggtccgcc ggaggtgaac acggccacgg tttggtccgg atggtcccgc
     4261 gcgatgcctt caatcttgtt gaacacgcgc tcctgcattt ggttccagga ttcgccgccg
     4321 ggcggcgaaa aaccgacggg atcctcatga aagcggcggg cgtccgtcgg aaacgttttt
     4381 tcgatttcag gccagaggcg gccttcccat tcgccgaagc acctctcccg gagggagtcg
     4441 gtatggacga cgcggccgtt ccagtccgcg agggcgatgt cggcggtgcg cgcggcccgg
     4501 tccagatcgc tggcataggc cgcggcgata tccagctgtc ttaaacgccg gcccacctgc
     4561 cccgcttgtt gctcgccgag cggggagagg gggacgttct gatgcccctg gatccgctgg
     4621 aggcggttcc agtccgtttc gccgtgtcgg ataaggacga tgtgtgtcgg catcgaaaag
     4681 gttttatggg cgacgggctt tttaatgaaa gtcccgcaac ggataaaaat tagcaatttt
     4741 gagagggtct ctcaatggcc cgcagggtct gccgcgaatg acgttcgggg ggccgttgga
     4801 tgacgggtat cgtcaaagag tggagccgga ccggcggcgg gcggattgtt tcttgcgtgg
     4861 gggggacacg attttgcggc gctggcccga cgcgcgcggg gagtcgccgg cggacgagga
     4921 gacgtccggc ttaggcctgg atgtcgaaga gttgctgttc gtcgcggagt ttttcgaggc
     4981 tgggcatgcc gactttttcc gccggcagca ccaccgatcg gccttctcga taaggagcca
     5041 gtaggcggga gatctcgtcg tcggtgtagt ggtagcgttc ccgcaggagc gcctgcagcc
     5101 agtccagcgc cgcgccggcc acctcgatgc gcacgcccac gtcgagcagt ttgaaggcca
     5161 ccgtcggcca gcccatccgt cgggccactt tttcccacca cggggcggcc agggcgtccg
     5221 ccaggatcca aaccttccag gcgcgccagc cgccgcttcc tgaaactttt tgaatatgtt
     5281 cggcgatgcg gtcggccgtg ggccaacgct gggcgtcgaa gagcaggtcc ggttccatca
     5341 tcgtttcggg aatgcccagt tcccgggcca gccgttgaat gaggagttcc cgctgggccg
     5401 cggacaggtc ttgtccggaa aaatccacca acagggcgaa catcaggcgg gtccctttgg
     5461 atttcaggga gcgtccgcgc tccacctgtt ccaggagagc ctgtcggccg acgttcttga
     5521 tgaaaagttt tccgtcccgt atttccaccc agccgcgcag atcggccatg accagctcca
     5581 cgttgctggg ggcccagtgg acgggcggac agggagtgat ctgcgccggg atgggaagcg
     5641 tgggggtggc tttcggttcg gaggtcccgg tggggaccgg ggtttctttt tccgtcgacg
     5701 tggcggtcgg ccgatcggac ggcacgtccg ttttggggtc ggaggggagg ggcgggacgt
     5761 cctccaccag ggcctgatag gcggcggcgg ttttcgacca cccccccgaa tcgttcatgc
     5821 cgtattcccg gcccatgacc gtgacgattt tcccaacgcc cgccctcgcc gtcagtttct
     5881 ccagggcttt cagggcgcgc aaccccgccc gcggcggggc gtccacgccg tccaaaacca
     5941 cgtggaggta aacgttctcg atcccttgcg cttttgccag gaggaggagg cgctccacgt
     6001 gttccaggga ggaatggatc tccgaggggg aaagggtacc gaaaagatgg agggcgtcgc
     6061 cccggctctt gagctgtcgc agggcttgga ggaaattcga gtgttcagtc aaaaccttgt
     6121 ttgtcggatc gaggcgaagt ctttcgaagg cgttcggggg cgcccggccg gccgcgatgg
     6181 cgaagaaacc ggtggaatcg tcggcgggct cttccggaga gagtcccagt tcttgtccct
     6241 gagcggccag gagggtcttc ggatagttct ccgccaggga gcgcaagatc gggagccggg
     6301 cggagcggag cgggtcggcg gggtccctca ggtcggggtt ctccccgagt ccctcgacaa
     6361 gcagaaggac cactttgcgc ggcgtgcggc cggcgaaata attttccggc aggagggaac
     6421 ggccggtcat gtcggccggg ggttgtatcc cgagggcctg aagcgccgtc ggcgccacgt
     6481 cggccaggga gccgttcgtc cgcaggagta ccgaggagcg cgggtcctgc ggcaggagcg
     6541 cggggtcgta gaaatggaag ggcaccgggt ttttcgtcga gagcggggga aggggattcg
     6601 cgccggcgcc gagatgttcc gccaggccgc ggtccgaggt gaccaggacg acggcgttct
     6661 cccgccgcgc ctgtgccagg actttttcca gtgaagagtc gagctgttcg gcgttttgac
     6721 gcacggcggc ggcatcgccg cgtccggccg cttccgccac gccgccgaaa tgggcgataa
     6781 cgagcgggct cgtccgcatc tcgcgggcga ccgtctcggc ggtttcctgg ggcgaagctt
     6841 cggcgctcag aagccggacg gtttccgccg gttcggcggc gtggttcccg cgcaaagcag
     6901 agacgattcg tggggcgtgt ttttccgaga ccacgacggc cgttttgatg ccgttggcca
     6961 ccgccgccga accgagagtc tccgaggcgg tcggccgggg gaggagcgga gaggattggg
     7021 gcacgttcgg cacttcggtg agggagaaga cgtcgggcgt cgctcccgcg gcgcgatgga
     7081 aaggagaaaa ttcctcagag gccggccgcg cgaaaggcgt caggagttgg cggggcccgt
     7141 cggcgcgcgc attgaaactc aacagcacat ccttgccggt gacgcggccc atcggccggt
     7201 cctggtcgtc gaccaggacg agagggcgca ggtattggtc ggtttcgccg gcggcgtagg
     7261 cctggcgcag ggacctcggc agggagcggc tgaggggcga gacatctcct cccgggcgga
     7321 gtcggacgtc gttcagaaat tcgccgaagg cgcgggccgc ctgaagcagc cgggacagca
     7381 ttctgcgcag gaagttgaat ccgatcagcg agagcacgcg cgaaagacga ggccgctggg
     7441 cgaggaaact cttcaaagat ggggccggcg ccggcagcgc cgggcggaca gcggccggcg
     7501 gggcttccgt cggcgtgttg ggttcggtcc actcgagaat gggatcgagc ccctggaggt
     7561 tgttcggcgt gaaggcgttc tcgaagccgt tctgttcgat ggcgagccat tcggcgaacg
     7621 tcatgtccgg atgggccagc cacaccttga cccgttctcc cggagagcct cgggtatgga
     7681 agatccagct gttgtcttgg gcgacggcca cccgcaacgc ctgcttgaag aagaactgca
     7741 ggaggtgccg cgcgtagttg ccgaagatgg agaggttcac gttgctggtg ggcatggtga
     7801 ccacggcgat ctgtgtttgg gccaggaaag tcagcaggtc ccgctgcagc cggcggaact
     7861 gaccgagttc ctctgccgag ggcggctgcg tgaaatccct ggggatgacg cccaggatgg
     7921 tgccgtgttc caggcggtcc agatggcccg aggtgacgat ctgcttcacg cgcagcatgg
     7981 ccggcatttc ttcgccttcg gcccaggctt ctcccacatg gatgctgttc ttgagaccgt
     8041 ttttcttcgc caagcgcatg gcggggagga gggtccacgg gaagaaatcc ccttcccatc
     8101 cgacggaccc gatgccgacg atgcgttcgg cgtcgtgcgg ccgttccgtc gcccagcgct
     8161 tcttcagatc gatcacgtcc tgcgcgcgcc gggtctgttc gcggcgggtg gcttccagca
     8221 ggaagacgag ttccgccagc gcggcgttgg tgagcccccg ctttttctga tgccaccgga
     8281 gttgttcgat gaaatcgccc agccagcgcc gttcctcgat tttttcctcc gaggagaggg
     8341 cgggatcgat gacgcggttt tccagccagg cggccagttc atccagaaat tcttcggtgg
     8401 aaagtccggc ccgttcccgg gaggcggcga attcgccgcc ggcccgctgg gcggcgtcca
     8461 cggcgtcttt ggtcagcacc gcggcgggtt ctttgcgggc caccacgatg gcggagactt
     8521 tcgcgtgtcc ggcggaggaa tcctccacgt cgtcgaaagc cgccatgtaa ttcgagacca
     8581 cgtcttccag ggagccctgt tcgccgggtc gtaacccgga aacttgcagc tccatcaggg
     8641 tgacgttgtc ccggacgaat tcttccagga cggattcgcg gaacgccacg cggttggccg
     8701 cgggatcgtt gcgcaggacg atctcgcccg tggcgaggaa ttccacatac gttttcaaac
     8761 tgcctttttt aggtttcggc cgatattcga tgaagttttt ggcttttttc agggcggcct
     8821 ggagtttttc gtccgaaccg aagaaggatt gaagcgaagc gacaaaaccc ggattcgtca
     8881 accggatgtc gtcctggggc agggaggaga gccaccgcga gagggcgtcc cgatcggccg
     8941 ggctgtccgg cccgccgaaa tgttcggcga aaaaggcgtc gatgtccggc acggccgacg
     9001 ggttctggtg gaggacttcc cgaaggaact gttgtctcgc tttgggattg cgccagtaga
     9061 ggaccatgag aaggagcgga gaaagggagc cgctgatgtg gcggtgcagt tccgtttttt
     9121 cataacgccg ggcgatctgc caaagggctt cggcgaagag tcccttgccg aggcgttggg
     9181 agaggctttt cggcgtctgg cgcagtttca acatgcggtt taattcgttg agggccgcgt
     9241 cgaccagttc atgttgcggt cccgaaagac cggggagggc gtaggtgagt tccttctggg
     9301 cccggtccgc caggtcgaaa acgttgaatt ggatgttcct gtggatgaac ggattgttgt
     9361 gcgggttcag aaggaaatca agggcctccg tgaggacctc ctgcgccgtc gaggccgcgc
     9421 gcagccggtc ggcgagcggc cgggcatccc gccactgata gatctgttgg gcgaaagcga
     9481 tggcccggtc cacgttcagg aggcgctcgc gtcgcgggaa gatataggtg ctgtaggcgg
     9541 gatcccggag agggttgagc ttcaacaggg tggccgcttt ccgggtaagc tcgtagggga
     9601 ggtcggagcg gtcgaggagt cccttggcga tgtgaaggat gacggcgcgg acctcctcgg
     9661 tctcctgggc gttgtctccc atgatctgga gccggaggtc gagggagtgt ccgaggatat
     9721 tgaagaccat cggctcaaac tccgcgaaaa agttccggat gatctgcggg gatatccccg
     9781 ccgtgcggcc cgtcagtatc cgtgtcatca ggggagcccg gtcgggggtc cggagcgccc
     9841 gatagagggc cagcacgtca aattcggctt cggtgggttt gatcccttcc cgcgtggagc
     9901 cctgtgtgct ctctatttga acggccagtt ctttgaacgg cagatgtttg gccacgctgg
     9961 gaggcggcag gccggtgttt ttcaccagcc agtctcgcgt gcgcgaccaa tggaaaaaca
    10021 gggagtctcg cgtcaccgac ccgccgggga tctggatgtc cttgcccagg aacgtgtagg
    10081 tgtcgtcgcg ttgcatggtc ccctcgacga tgagtttgcc gcggagccgg aaatagccgg
    10141 ggagggggag gagtgaaagg acgcgtttcg gcaggaggcg gcccaggccc gtcatcaggg
    10201 gttggagcca gcccgtttta cggcccggcc gggttccgcg gggataaatg gattgcagga
    10261 gtatccggtt gaggacgtag gtgtcgaaac gggacagggt cccggcccgg gctttttcga
    10321 gcatctgccg agtgcccgac ggcagcgaca agccggcggg gaggtgggcc tggaggtccg
    10381 gcatttggat caacgcgttg agggctttga ggagcttgcc ttcaaatcct tttttccggg
    10441 ccaggtcggg caggtctttc gccgtcgcgc ccagtttctg caggagcagt tcggcgaggc
    10501 cgtctcccct cacgacggag cggaccagga cggacacgtt caaattcccg gccgagaaga
    10561 aggggccgat cttgttgatg aaacgcaggt tggcgctgga ggcgattccg agggccaccg
    10621 cctcaaaggc ttttcgcacg ccttcctggt tctcccaggg cgtcatcgtt ccgatgagga
    10681 tgcgttggct ccaaaggtcg tccaggactt tctgcatgcc ggccggggtg gattccagat
    10741 cggcgcccgg cgaggattcc tggaacacca tggagcgcca ccggaagttt tctttttttc
    10801 gggttttcga gtagcgtttg agcatctgcc gcaggaccag catgtggaat tcctcgttga
    10861 cgggggaaca tttccattcg gactcgttgg agatgaaatc cccgccgcac ccggtgatga
    10921 ccacatagac gttgcgcccc gcgaaacgtt tttggaacag gtcgtattcg gcctgggcga
    10981 cctcttccgg ggaatccttc atgccgatca taaagaaatt gtccgcgccg taattgtcgc
    11041 gcatgaattc caccaggttg gcctcggcga tggggatgtc ggcgcccttg atgcgcgtcc
    11101 gtcgggtgac ccggaagacc tggccgttga ccggctcgat gaattcagtc tccgccagtc
    11161 cccggccgta tcccgcgtcg tcgtcgtaga cgtaccgttt ttgcgcgggg tcccacttcc
    11221 acagcgggtc ctcctccgtg gcgttctctt ctttgacgaa cttgtcccgg gggaccttca
    11281 ggcccgcttt tttctggtgg acgctgatga gcaccacctc cgcgcccatg gcccggtatt
    11341 tttcggccag gtagatgttg ttgagcacgt ccccgccgcc gccggtgttg cgcatataga
    11401 ccaccggccg ctgggccggg tcggacccgt ttttccgcca ttgcgccaga tcgtattcca
    11461 gtttgccgag gaagttgtcg gaaatgtccg gcgtttccat ctgcgcggga tcggtgacgg
    11521 gttgataaag ttcatggcgc gcccagaggg agggggccgc gggtttgaga ctgttgcggt
    11581 aaaggaacac gaccgacagc cacagaacga cgggcacgct ggccggcatt ttccagaagt
    11641 aatgggagaa acggaacgtg cgtttcgggt ctttcaggcg gtcgttgaga agcaccgcca
    11701 ggacgatggg acggaaaagt cggacgacgg ggtttcgtgt cgccgccgcc cagcggcggg
    11761 cgatgtagac cttgttcttg gcggacacga ccaccagcga ggcgttgttg ccgaaccgct
    11821 tttccatctc ctcgtcggtc tggtagacga cctccagggg gagggcggag cgttccgtcg
    11881 tccaggtccg gatgagttgt tcgatccggg gatccaccgg cgcggtgtcg tccagcccgt
    11941 gcaccgcgtc gacggtggtt ccaacgacgg cgtggatcag cgtccccagg ccgaagaaag
    12001 aggcggtcgg caggacccaa acgatgttct cgcttcccat ccacagggcc gcggccaggg
    12061 ccagaacggg ggcgacccac accaagaggg aggaaagggc gaccgcccgg tgggcgtgct
    12121 gggaacggta ggtgtactgt ttccggatgt cccggtcgac gcccgtgcgg atcttgtcca
    12181 ccagcgtttc cagatagcgc gcggtctcct tgggttcttt cacgtgggcg tagccgatgt
    12241 tttgatgctc gcgacggacc gggttgtcgt tcccgaattt aaagaacgag tcgccggaga
    12301 agaagatctg gtccggtttc aggccgaagc gctccatcac gcgccccatc gcggctcctt
    12361 tgccggaagg gtccacgccg atgtctccgt ttccgcccac ggtcacgttc agttgaacgc
    12421 cccaggcgga caggcgcggg tcgttccgga gtgcgtcctc gatgaacagc gccaggggcg
    12481 cctgttgggc ggcctggccg ggcccgggat ccaaaacgtc gccgtcgtgg aaaggcttat
    12541 ggcgggcttg gtcgaggtgg aaggctttgg cggctttttc gaacacttcg gacaccacgg
    12601 tcctcaccgc cgtcctcgtc cggggatccg agaggaccgg gtgttcccat tccttgacgg
    12661 gggctccttg ttcgtcgaac cgatagaagc gcgagcccga ccctgtgaag aggaatacgt
    12721 tttgccggtg ttgtggcgac agacgggaga tgaatccgcc gatctgttgt tgcaggaggt
    12781 ggtccatgtc cgatttgccc gtgatgaaga cgagcggcag tcccaaggcc agggcctcgc
    12841 gcagataggt ttcgttgccc ggggtgatgg cgccgttgtg gggcgccagc gtgttgtcga
    12901 tatccgtggc gatgaggcgc acccctcgca ggaggccatg ctggagccgg agatggttca
    12961 tccgccgccg cagataatcc tggttggaat acgcatagcg gcggtgggcg tggatgcggg
    13021 ccgcctgcgg atggaagaag ttccctttgg cgacgaagag cctctgaacg gagacatcgc
    13081 gacccaggga ggcccatttt tggcgatacc cctcaagaag ggaatcgagg gcgttcttga
    13141 agccaacccc gtaggccgtg tttcggcgca gggagagaac gaggtccacc ttctccgccg
    13201 cgccgaggcg gttgccgtcc gtggaggtgt ccgtcagggc ggccgtcagg gcgtccaatt
    13261 tcttctgaat gaagatcaag gtatcggagg cgttggtctg tgcctcggga tggccgggca
    13321 cctccgtcag tatccggagc cggcgcgggt cggcggcgtc cgtcaagacg gcatccagtt
    13381 ccaggggttt ggccgtgtcc tcttcccggt aggatatcca ttgcgccaaa cgttccggaa
    13441 catgtcccgc cgcggcggaa cgagcggcgt ccgccaaaag ctccgccgcc gacaggaacg
    13501 ttcggaggga tttcaaagtg ctctcgtccc attcgacggt gttcgccgga acgtcaaaga
    13561 cgcccatgtc catggatcgg atgacgcggg aaagtcccgc caccggggcc agccgtttga
    13621 tgtggtccac cagggtgtcg tccacgcggg ggtcctggaa tatttcgatg aggcggtgga
    13681 tcgcttcgaa atcccacgcg tccggcgatt ggcggagcag gatccggatg tcctgcatca
    13741 gttccctgga gagatggatg gtccgaagcc agcggatgat ctccgggttt tggcggcccg
    13801 tctcgccgta aacctcggtc agcaacgggg cggcggagtc gaggccggcc cgggggagcc
    13861 actcccggga ggaaacgagg aactcctcga tccatcggcc caggagcggc cgggaactgt
    13921 tttgggagag gtgttcgcgt tggaagcgga tgtggctcaa ttcatgacgg tagatggcgc
    13981 gctgcaggaa ggggggcagg cgccgcatga tctcggcgcg gacggacacg aaccccattt
    14041 gggttccggc cacgcggtaa cggtccaaaa atcgcatcca acggtctttg ggcgtgggcc
    14101 gggcggcgtt gcgcggggtg ggcggcagaa ggggcgaggc gttccgccat tcggggatca
    14161 atccgtaatc gggcagccac tcgtgcgcgg cggcgaagat gaggccgaac tgcagaagga
    14221 accgggacgc cacgatggct ccggggagga acgcccaaac gcccaaggcg tgtcccaaca
    14281 gggaggccac gccgagccac accgaggcca cggccagcgc gttcaaggcg gatttatatt
    14341 ttgtggaagg cggattaatt tccacgttca attggaaatc ttccacctgc cccttgttga
    14401 ttttcaccgt cacttcattg aatatgtcca agccctcggc ctggtcgccc gatacttcga
    14461 tcaccaccat cgggttttcc aattgcacca acgcccgcgg cggccgtccg ttcgtccccg
    14521 gcgtggagtc gatcacgtag cccgtcaacc tctgtcccgc atactccggt ttcgacaatt
    14581 cccatatcgt tccgaacgag gccagctcat tttccatggc cttcgccgcc cgctcccggg
    14641 cgttcagatg ttccacatat tttttcaaat cggcaaatcc catggcccgc aagtccgcca
    14701 ggatcgcttc gtagattttc ctcggatcct gtcccgtcaa ataggcgttt aagagcgcct
    14761 ggttgtacat gtccgaaaaa cgtcggatgg gggtggacgg atgatcgtac tccccggcct
    14821 ccagcgccaa tccctggtgc ccctcctggt cgataacgtc gtatcgcgcc gacattctcg
    14881 atttcaaaat tttcaagctc aacgcttttt tctgccacca agacaggtct tctcgcgctt
    14941 ttaacgccgc caaatattcg cttatcgttt gccctttccg ccacggggcc ccgatccgct
    15001 gcaaatcttc ccggatttta tcttccttct cctcgtcctg ttcgggatgc acccggctca
    15061 ggtgaggaac cccgtactcc ttggtgatcc gcgtcaatat ctttgccagg ccctggttgc
    15121 cgtacacctt caactcctcg atcaaccggt gggactcttt caaaaggggt ccctgttcct
    15181 caacaacgat ccgccagtcg ttgggaccca tcttccgtaa gccggactcc gggaattcaa
    15241 ggtccatctt ccctcgttcc tggtctttcg ccgtcagctt cgaggccaat tcccgggaca
    15301 acgccacttg atccaaatgc gtgatgcccg tgtccgtttg ccctttccac agttccgtca
    15361 cctggtcata ggtatagcgt ccctgcacgc cgacgagccc catgaacacg tccgatttaa
    15421 tttcgtttcc ttccgcatcg aactccatct tcgtgatcat cgccaagctg gctttccccg
    15481 ccagcaaact cgccacatat ttcgatatca cggggtggtt catcggtgat tcgcccagtc
    15541 cgtccgcgat atgcatgtaa aacgtgttcc ctattttcgc cgccgtccgg aacgccgggg
    15601 tccccggcag cacgtagttc ccaacgtccg ccgtcgccag ataccaggtg tagcttccgt
    15661 ccgcgtgctt ctcaacgtga agagcatcgt ccaggtctcc cgccccctcg ggatcgatgg
    15721 tgatgaacgc tttatccatc atgtcttcga tccgcagccc ggaggggttc cccgccagtt
    15781 cttttcggag gcgttcgaag tctttttcgg gagatctttc cgactcgatg cttcggccaa
    15841 cttcctccga ttgtttcacc acctccgcat caaaatagcc ccgggctccg gcccgcagcc
    15901 cgatctccag tttcgcgatc tccggcgtta tttttttgcc gcggaggatg atcggcgtcg
    15961 cgatgaatcc gctcccgtga ggcttcacaa acgcctggac gatgtcttgg tcctccattg
    16021 gcctcccggc gacttccgtg atttccaaat ccaaatcgtc atacactctt tttgatttgc
    16081 ctcccggccg gaacaacggc gccaacacca gcttgttccc gcgtttcagc acccgcccca
    16141 gcatcatgtc catgggatat ccgcccacag gccttacgga aacgatctcc cgctccggtt
    16201 ggctctggcc gcgcttccct ccctggcttc cggctttttc tttcttccct tgtttcccct
    16261 tctttccttt ctgggcctta acggatcggc cgcgcgcctc ttttgccgcg ctttcgcgat
    16321 acacaacttc caccagcgta ttcgatagta tcccttgccc cagggcgggc gggacgaaca
    16381 gttttgtcgt tttctttttc tcgtccggcc gcgtcgcctg ggcgccttgc tccgacacct
    16441 ccacgctcat ccgcccgatg gcgtcgcgcc ggaagagtcc ggtggcgacc ttcgctccgg
    16501 tcggcagcgc ccttttcttg gattggctga atccgcgcca cgccgcttcc cattccgccg
    16561 gcagggtgat caccccttgc gccagcagat attccaaccc gcgttcctcc ggtcccaggc
    16621 ccatgacgaa ttttttcaac atctccagcg tcaatcggcc cgtcccgtcc gcggccatgg
    16681 tcaggaaatc cgcccccgcg gtttttgggg cgaccgcctc ggccgccgcc gcttcgactt
    16741 ttttcgtcgc ttcggacccg ggcaggaact gggccatcgt ttccacggcc gagaccgctt
    16801 tctgggcgat cggcgtggcg gccggagccg gttttgtcga cggggcgctg acgagccggc
    16861 gctcggcggg cacggcctgg tttatttgcg cgggagcggg aggggtgtcc cagaggagtc
    16921 ccgccaggtc ggccagctgt ccgctgtgca ggtcgaattc ggcggccagc gggacgagga
    16981 agccgatctg ttcttccgcg gtatcgagct tcagcaaaga cgcttcatcg aaccggcccg
    17041 ccagcgcggt ttcccatttc caaccccact gaaccaggct cttcaggaag gccttccggc
    17101 ggtcggcgcc gtcgcccggc tcgcttttga ctttcgcaat ggcgtcggcc agtcgggagg
    17161 cggcgcgcac ggcgtcgtcg atggccacgg tttcgagcca gtcgtccacc gggcccatgt
    17221 ccgtcaaatc gtcgacgaag acgattttcg ccggcaaccg ataggtttcg gtcatgccgg
    17281 gttcctgttg aacgagatat tctttcagtc gggtcaggcg gttctgttcc ctctcgaaat
    17341 ttttgcgcgt ggggaccgcg gtgccgatgt ccatgaagaa gaggaaaccg tcgagtttta
    17401 tcgggaatcc caggccgcgg ctgacggaat cttcaatggc cgccagatgc ttcttgtaat
    17461 agttcatttt gtagcccact ttggagctga agatctcttc gggcttgagc ggcgtgcggg
    17521 ccgacttttc ctcgcttgaa atgaccttga tccggatcgg cacccgccgg cctttgacct
    17581 cgacaaaaac ctgttgttcg ccgtcgacga tctccgaccg gatcggcacc gactccccgt
    17641 cgatgtccag ggtgccgctc ccgccggcca tgttgatcgg agacaggcgc cattggccct
    17701 ccacctgcca ataggccttt gccacgacgg tgtccagctc ggtgacgtac atcccttccg
    17761 gcgtgagcac ctcgctcccg gcctcaagga tccagtatcc ggccgtgcgg aacatctgct
    17821 gggccagcaa ctccaaatcc aggccgacca aacgggaata cgtcacgcct tcgaggatcg
    17881 ccttgacggt ttcctctccg tagagatccg tcaattcatt gatgcttcgg atcatgtcct
    17941 gatgggtccg ggggcgcgcg gcatcgtcga catggacgat ttttatccgt tcccggcgga
    18001 tccccatttg ggcgcactga cgatcgacct cttcttcgac gaatcggtcc agttcccggt
    18061 atccccgcgc atcgaacccc aggatgattt cgtcgagggc ccccatcgcc ttttctgcgc
    18121 gtccgcccgg cttgatggtt tcgaggaatt gacgcagttc gcctttctcg cccgccagcc
    18181 gtcgttttcc ctccactatt tccgattccg ggttggggga cttgtggaaa taggcctgga
    18241 tggtgtggac gagcgtcact cgctgcgccc ccggcacgtc gacgtttcgg tccgggttgg
    18301 ggggctggtc gtcgctcgtg taaaccccgt ccagccacgt gagggtcatc gacaggtggg
    18361 aattggaaga gaggattttg ctgacgttgc tgtgtgtcgc ggcattgagg atgtgcatct
    18421 tggcccagat ggcttcgtag gtgtcccaca tgacgccgag ggattcttcc acgttcacgt
    18481 cgtgttgttt gagccaatcg atcaggttga aaacgtgttt gacgagcttc gccgctttcc
    18541 cgatgaaatg gaccccctcc aggtcgctgg aatcatgaac gggcggcgcc cggggggacg
    18601 tccgctccaa aacgcgttgg cccgcggtca ccaccgccgt cgtcggaggt ccggccgcgg
    18661 gaatagtttg agccaggtgt tccacggccg tcgcggccgc gcgagccagg ggcccggcgt
    18721 tgttcggttt tgtcgccgac aagagggggg cgttccgcac ggccgccggc ggaacgaggc
    18781 cggcgggcgg cgtttgcacg ggcacgctct ggtccgcccg ctcctcgcct tcgtcggcca
    18841 cctcggcttc ttcggcggcc gacggctgga tcggcgcgtc catttggatc agtttgattt
    18901 tcgggttggc ggcattcagg tggaggaacg cctctttgct cacgcggttc cgcatctcgg
    18961 ccgacgccgc ccggaggatc aaagtcacgg cccggctttc ggaatgccgt tcgaccgcga
    19021 cggcgtcttt gtcgatgccg agccgctgga tcatttggcc ccgcgccttg gcgaacgctt
    19081 ttttggagat atcggcgcgg accacgaggg cgaagggggc gacgttgggt ttggaggatt
    19141 cccggacggt ttctttgggg ggcgtcggcg ggacgacctc cgccggcacg ttgttgaaat
    19201 cggccgtggg atccaatccg cccagaacgg cgcggaaggc tttctcggcc gccgcctcgt
    19261 cggcctcttt gcggagggac aggttgacga cgtagacggt cgttttccgt ttcccatcga
    19321 acacgacctg gactttggcg taaccgctga ggaccggcag tttttgtttt tgaagggcgg
    19381 tcaccgcccg cttggtgagg gtgttgaccc ggttgagttt gttgtcggag acgagtcgga
    19441 cggccagggt cttgtcctct cgggccggcc gggacgggcg gccccgcgcc ggtttgccgg
    19501 gtttcgactg ggagacggga tgggaggtcc ttgccgcggc ggcggtggcc aggtccgcga
    19561 gcgtcaatac ctcgccgttg cgtttcgcct cctggtatct ccgtgccgcc agggccagac
    19621 ggatccagac ggattcccac atgaggtcaa tgaagttggc cggttccagg aacgggttgt
    19681 gcaccagcca gagcgccagc cgctcctcct tgctgcggat gttgccgttc tcgtcgcggt
    19741 cgatggcggc gacgcgttcc agaaatttgt cgaacccgcc gatttccagg atggtctggc
    19801 ccttgtcgtc gtcggagact ttacccgact gcatgatgag ctgttccact cccacgccgg
    19861 tggggccgcc ggaggacagg gggctccgct tcttttccca gtgtttgtac gcccggacga
    19921 cctctttgaa gaacttcttg gcgaaacgga tgtcctgcag aagccggtcg gccgccgcct
    19981 gcccgcccag ccggactacg tgcgccattt gcaccgcgaa atattcggga tacgggttgg
    20041 cgtatttgtc cgaattggtg aaggtgatgt ccatctccac cacgggtttg tggtcctcat
    20101 cgtagatttg aatggggagc atgtacacgc cctcgtccgc ctgatcggtc tggacgttgg
    20161 agagggtggc gaacagggaa agggggtcgg ccgcgacggc gttcttctgt ccgacttcga
    20221 agcggaggcg ccggggataa aggttcagtt cgcccagttg tttggtcaac gtttcccgaa
    20281 ggaccttctg cagtcgggcg tggtttttgg agagaaaatc cgtcaggtcg gagggggtcc
    20341 attcccgcgg aacgcggacg cacaggtcgt gatcgggatt gctgccgggg gtgtgcgtga
    20401 ggcgggcggt ggatccgcgc gagatgacgt cgaagtcctg ccgcgggagc aatgaggcga
    20461 cggcttcgcg gatggcggct tccagcgctt tgtcggtccg ctgcagccga tcttcttcgc
    20521 cgggggcgag gacggcgggt ttttccgtga tgaggggatt gaaatcggcg tcggcccgcg
    20581 cttttttggc cgcgacctgt ctgatcgccg ggtggcggat ggagacgatg aaatcgaact
    20641 gccgtttgtt tttcgtctcc cggcggatgc ggttgtccac cgctatcaat gttttcagca
    20701 ggatttggcc cttggggccg gccagcgctt cctgcagttt cgccgccgct gccgccgcct
    20761 cggggtggtt tccgtgcatt gcttccaagg catagagctc gatggttttc gggtccgtca
    20821 ggaagtccgc cacctgcgcc gccgaaagtt tcaaggccag gtcgccttcg ccggtggtct
    20881 gcatggggat gttccgcgat tcggcgaagt cgtagagcgc ctgggtgagg cggcgattgg
    20941 cgtgctgcat gtggatgacg aagttgcgga ggccgctgag gcgtctcttg atggcagggt
    21001 tggagaaagg cttgccgaaa ccttccaggg ttccgtagat gaattgttgc gccaaaaagc
    21061 aaaggtggca aagcagccgg ttgtgggcgt ggccgtcttc ccggagcccg attccggcga
    21121 actggccgta gatgtcttcc agggagccca ggtacttgaa ctcgaacatg gagctgcggt
    21181 tgatggcatc gaactccttg atgccggagt gcccgttggg ggcgggtttg gcgccgagga
    21241 tctcccagtt gacgcccgtg aacacgcctt gaacgccaaa acccgccagc gcggtgcgtc
    21301 gcttgaagac cacggtatgg agaccgttca cctcgcccca gaaaccgccg tggttggccg
    21361 gatcccggaa cgcctccagg agggagggga ggaggtagct gttcttcgtg ttgcggagga
    21421 aggggttttc gggttcccag gccaggaggt cgctcaattc cagcaggagt tcggcaaaat
    21481 cgctgcgata atagcggctg tccagaatac ggtccacatg gtagatgaac ccgtccagca
    21541 gcgagaggag ggggggggtg tcgcgcggcg agtcgttccg cgccttgacc agcgcttcca
    21601 ggaaccagag cagcagcccg cccgcgtcgg tgggcagagg ttctttcagg gcggccaggg
    21661 tccgcagagc cgcctggatt tcgccggcgt cgacggactt ttctgatttc agcaggacac
    21721 gccagaggat gtcttcccgg cgggcggggt cggccagggc cgccgcggaa atgacgatgg
    21781 tggtgccgaa gatccggatg tcgtctccga gggtggcgga ggtgacgagg atgttccccg
    21841 tttggggaac atggtcgagg gcctggtgga cctcttcgag aacggcgttc agggcgggac
    21901 cgtgttcggt ttcttggatg ggcaccacgg cctggccctg cccttggacg cgtttgatcc
    21961 atcgggcggc gttgcggtcg gcttgggacc ccggctcctg ggattttttc cagcggatcc
    22021 attgggacac ctcgaaggtg agcacctttt gatcgtcggc cgtcatctcc gccagggccc
    22081 gccggtgggc ttcctcggga gggacgcctt tggcgatcag gctgatttct ttcaactcat
    22141 gtttcaaaac gattttcaag accgcggggg ccatctcgaa gagggcgctc tgcaacgtca
    22201 ccgcgaatgt gttgtcgggc ccggcgcgga tggtgtttcc caggagatgg aggtcgtgag
    22261 agacgatcaa ttcgggcgtg tcggcgatgc ggaggtcgac ctgcacgggg acctcgctgt
    22321 tcatgagcgc ggagaggccc ggggcgtcga tgttctccag ccacagcccg ttctctccca
    22381 ggaaggtgtc gttcaggtcg gccaggatgg cccggtgttc ttccgacggc tccaccgcgc
    22441 gggacgagaa aaagcgggag agggcgtccc acggggaatg cacgaaatcc ggaagggagg
    22501 cgatccccag ggtatggatg atgtccgtgt gtgtttccca gaaatttttc agcagagtgg
    22561 gcagatcgcc cgtgaggtaa tgcccgcggg cgaaggggag gtcccatttc ggcgatcgca
    22621 ccagcgcggg aaaatccagc gtggacaccc agaactcctc ggcccatcgg ccgatggcgg
    22681 tggcggaata cgtcgggtgg cggtgttcga atttgagatg gttgacctcg tggcggtaga
    22741 tccagcgttg catgccgtgc ggcagccgcg ccatcaacgc gtggttgacg gtgagttggc
    22801 cgtcgcgcag ttcggcgaca cggtatttgt tgagggaggt gcggatcttg ccggaaaagg
    22861 aggaggggtc ggcgacggcg gcgggggtga gcggggcggc gtcctgccat tcggggatga
    22921 ggccccggtc cggcagcacg tcgtgcgcgg ccagcacgat cagggcggcc tgggtgagat
    22981 gcggcagggc cacggtggcg ccgagcagcc cccacaggct attgataagg aggacgccga
    23041 gggaggccac ggccgcgccc caggtgacgg ccgtcaccga agcgttggcc atggacttgg
    23101 ccagccggcc gatggttttg aatccgccgt acaccgcgga cacgcttttc gtctcggaga
    23161 ccagcgcttc gcccttggcc acgatcccgt ctttctccag ttccgccagc cattggcgca
    23221 ggagggggtc atcgatggat tcgatcaggc ggaggaccgg catcaccggc tctatctttt
    23281 ccgcgatccg gccggtatcg tcggggtacc gccggtcgat gtgttcaatg gccttggcca
    23341 ggaggtaagg ctgcaccttc atgggcacgc cggcgcgatg catggtgagc gtcaggtgga
    23401 ggatgaaatc cgaactgatc cgctcggcga gcttgtggat ctcgtccgtg tagaaatcgt
    23461 agccgtgcat cacggggtcg tccaaaaacg gccgggcgtg ggtggagagg ccgaagatgc
    23521 cgatgagctg gtccgcctct ttccggaact cctcggcgga gacgccgtcg cgttccaggg
    23581 attcccggaa gccggccaac gcggccgcgg cggccagacc ccgggtttcc tcttgataat
    23641 attcggccgc cagccggccg aaataatatt tttcggagac cgtcatgtcc gcaaaaacgc
    23701 cgtcgccccg gcccgcggcg aaccgtttga ggaaatccga ttgccggtcc gtgctgaaga
    23761 ccgtatagaa cacgtccttg aagaccttgt cggcgtcggg cgttgaaagg gcttcccgcg
    23821 ccaggtcgac caggcgggcg acgtagtccc cggtgcggtc gaaggtcagc caggggatct
    23881 cttttttgcc cacctggacc atgaaccggt cgaacacctc ggcgttgtat ccctcgtaat
    23941 cgcgggtggc ggtgtggcgg tcgatgacat ggagctcgcc gatgtaggtg cggaaatgtt
    24001 tttttcggat gagccagtcg aacatgtggc ccagttcgct gttcacggcg aacacggaac
    24061 cgacgagttg ggcgtcgtcc atcttgtagc cgccccggaa cgccccgccc acccacatat
    24121 attcttcgtc ttcgatgtcc acgtcgtggc ggctcaccag gttcttgggt atcaggccga
    24181 ggtattcctt cggatagaaa cggagcgtgt tcgcgtagag gagggcgaga aggtgctgtg
    24241 tgaggtcgtc cggtttgagc tcctgccggc ggaggaggga acggactcgg gccgtctcgg
    24301 cttccgacgg ggcgtaatac cggtcctgga aggagatccc gaggagagag cggaggagga
    24361 tgtcctgcgg tttgggacgg gctttcagcg gaacgcccca cttcttcagg tgtgcttcga
    24421 gggcggggca gagttcttgg gtgatccagg cgttcgtgtc gccgtccagg ggaacggcca
    24481 ggagcttttt cagcagggcc tggcgctggc cgagcggcag gtagaggttg cgggagacca
    24541 gcatgagaaa agagagggcg gtctggaact tcaacgccgg cggaccgcgc ttgcctttgc
    24601 cgagggcgtt ggtcgcctcg atgcgtttgt ggacctgcca catcagcggt tcgccgccga
    24661 aggtgtcccg gacggatatc aggattttcc ggtgtttgtt gagggagagg ggctcgttgt
    24721 agtttatccg aggtgtccat ttaaatcggt cccggggggc cggcgtgagg gcgggcctcg
    24781 tttcgcggcc ggcctcttga accaccgccg ggcgggcgtc ccgggcggag atcaatgtct
    24841 cgacgatgtg ttggcggagc agaaggtctt tcgcccggag gatttccata tagagtccgt
    24901 cgaggagggc gttcacctcg attttatcca ggtcggcggt gttcagggtg ggaggaaggc
    24961 cggcttccgc caggagcgac aaaacctggg cggcgcggcg gcgggcggtg ttcgttgtca
    25021 gggcctcgtt gaaccacagg tcggatacgt tctccagcag tttttccgcc gcataaatct
    25081 tcaacaggcc cggagccggc ggaagggatt cgatgagagg ttctccgtgg agggtgagcc
    25141 cccatcgcgc gatgcgcagg agggcggcgg ccagggaacc ggtgcgtttt tcggggagga
    25201 gcgaatcgag gggcagcacg ctgacgtccg ccgtgggcag gccgtccgcg ccgggaagcc
    25261 gccgtcgggt ctccgacggc cgtccgtctc ctttcacgag gaggacgagg tcgatgtcgt
    25321 tgggggtcgc tttcgggtcg gtgaggtagc tgccgtggac gatcacgctg acgatctccc
    25381 ggcccggggc cagttcctgg agatggggtt cgatcgtttt caatgcgtcc agccgggatt
    25441 ttattttccg ccagtcttcc ggagagaagt gggagaagcg ggggttggcg cggaatcgtt
    25501 cgaaggcggc gtccatggaa ttcacgcgcg tctccgttcg ttccagggac caatcgaaag
    25561 tcgggttggt cgggagtggt ttgaaaaatt cgcggatatt cctttcgccc acgcccagcg
    25621 cctccaaaag ccgggcatcc tccggcgcga ggtcggcgct cgtgcggtgt ttcatcagcc
    25681 ggtccgccag cgcgacgaga tttcggtcgg acaggaggtc tgttcgccgg ctttcttcct
    25741 gcggcgtccg gcccatgcgt ccccttcggc cggccaagag cgccaagaac cccgccagga
    25801 gggaggacag cccgtatttc aggagggcgg ttttttccgc cagccaatcg aacgttttga
    25861 acgccgcggg gaatcgtttc atcggcccga cggcgtccgg ccgccggagg cccggggtgt
    25921 tggaaaacag ggtgttccag agaaattccc ggaacgccga ggtccccggc cggaggtccg
    25981 tctccagcgg gacgtcgcgg aaggtgaggc ggttcccccg ggcggcatgc aggcgcagat
    26041 agagtcgtcc gtctttttcg aacacgaact gttccgccac gaagaggacc tttcgccgaa
    26101 ggaggctgct gtaaaacgtt cgattcagga ggagggccct gtttttgacg aattggccga
    26161 aggtgtagga gaaatcctcc cattcgccgt cctggggcag tccgacggtc tggtcccata
    26221 tcgcgtcggt gctttgcagg aaaaggtttt tccaatacgc gcgttgttcg tcggtgaggc
    26281 cgtggaggta gtagtgatgc ccgacttcat ggacgacgac cgaggggtcc acgccgttga
    26341 gggtgttgag taggatttgg tccaggaggg acacgtagcg gccggccacg tcgtccttta
    26401 tgtccggcga gagccagggg ccgagcacga cggacgaaag accgtcggtg tgtcggggaa
    26461 ggaggaccga caaggcgttc cgggcgaccg ccgcgttttc aggagaagtt tgggccgaag
    26521 cgacgagtcc ttccgagggc gcgcccgccc gccgcgtcgc cgcgttgaag aggggggcga
    26581 ggagcagata aatcaacagg aacgggacga ggaggtggag tgtttttttc tggaaggaag
    26641 aaagttcccg cccgccggcg aattttcggg cgacgcgcgt gacgatgaga tgcgagatcc
    26701 agaacgacgt ccccggcacg agggtcagaa ggacgatgga ataaaggaaa gttcgggcga
    26761 gatagacgaa ggacccctgg gaaatcgcgg gaagggccca ccggctggcc agcggaacca
    26821 gcgccggcag gcggagggtt tcggcgatgt cgccgacgga gaactgggtc aggaggagcg
    26881 agaaggtccc tgtcaggatg gcgataacgc cggcggcgcg gagcaggttc ttccaggaga
    26941 gcgcgtcgtc gaccgtgagg gaggggagga tttgccggag ccagcgccgg acgactttcg
    27001 gcgggctctt gttctgcagt tccgaggcgg ccggccggtc cagcacgaac gtggtgtcgg
    27061 gatggtattg caggacgctg gccgggaggg agacggaggg tttctcttcc gccgcccggg
    27121 ccacggccgg ggctttgcct tccccgttgg ccaacaggaa aatcgttttc gcggccagga
    27181 tggtggccat cccctggctg agggcgcgcc gcggcacgtc ctgtttgtct ttgaagaacc
    27241 gggaattgtc ttcgatggtt tcggcggtga ggtccaccac gcgggtgcgc gagtcgaacg
    27301 ccgccccggg ttcgttgaag gcgatgtgcc cgttccggcc gatgccgagg atctggatgt
    27361 cgatcccgcc ggcctcccgg atggcctgtt cgtatcgtcg ggcttcggcc ggcaggtcgg
    27421 gggccttccc gttcaagtaa cggatgtgct cctcggccac gttgacgtgc cggaggaagt
    27481 tttcgcgcag ataatattga tagctctggt cgtgcgtgcc ggggagtccg acgtattcgt
    27541 ccagatggaa gacgcgcacg cgggagaagt cgaggccggt ttcccggtga aggcggatca
    27601 gttctttata gacccccagc ggggtgctcc cggtggccag cccgaggacc aggtcgggtt
    27661 tttgccggat cctttcggcg atgcgttcgg cggtccagcg gctcatggcg tcgaaactgt
    27721 ccagacgcac cacggtgggc ggggaaggct cttttttgag cgtcgttccc tgggtcgggg
    27781 cgttgcgcag caggcggcgc agcgaaaaga cggtgcggaa acgcgccgtg agccattggc
    27841 ccaccgttcc caggaaccgc cgccgggcct tttggccggc cgcgtcgttt ttcagaagaa
    27901 cgccgagctc ctgttgggac tccgccagcg cgtcctggat tttctgcgtc gacagcgtga
    27961 ggggcgacgg ccgcgccgcg cccgccgagc ggtcgagatc cacggcgtcg aggaacggcc
    28021 agagacgggc cagccggtcg gcgacgtcgg cggcgttttc aaggggggcc cggcgcatcc
    28081 actcgcggag gcccgcttcg tccgaggaga cgacggtgag gtcttcgttc gcggagagac
    28141 ccaggaggaa cggcttggcg gcgccggcca tttgcaccgt gccggccaca aagcgtttgc
    28201 cgtcgcgttc caggacgacc cattccgtgc gccgcaggaa ttcggagaac tcctcgcccc
    28261 aggctttttc ggatttcagg aaggcggcgc cggcgtcttc gcccagaagg cgccccatgc
    28321 cgaagaggaa cgtggccacc ttcgcctgga acgccggtca agatggccga agatccgacg
    28381 actgagccag
//
LOCUS       DAOTFD010000011        28134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1102_length_28134_cov_3.876990, whole genome shotgun sequence.
ACCESSION   DAOTFD010000011 DAOTFD010000000
VERSION     DAOTFD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 28134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28134
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_28134_cov_3.876990"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="P5079_01430"
     CDS             <1..610
                     /locus_tag="P5079_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY28679.1"
                     /translation="GELAFLGAPELVRLLSDASRAMCEGEIGQIERRFHFDLSEEEYL
                     SFLDKKTASLMAASCRAGGAAAGLSRKQQEALETFGRRLGVAFQIVDDILDLEGDEAV
                     TGKTLRTDLQHGKMTLPLIRYRDRLGSRSERKKFCESLRDGAGGFSGLIGKIRDAGAI
                     AESRAKARLFVDEALRALDELPGHPARQILSDVAHRLTLRRF"
     gene            complement(693..1010)
                     /locus_tag="P5079_01435"
     CDS             complement(693..1010)
                     /locus_tag="P5079_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004963273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRY28680.1"
                     /translation="MTAIDPEFQKNRTKVGEHAGHAVWGPEEKPAKLGIHGTWVAVDF
                     DLCIADGACLDACPENVFEWLDTPGHAASAKKADPAKEDACIFCMACEAVCPVVAIKI
                     TPK"
     gene            1110..2048
                     /locus_tag="P5079_01440"
     CDS             1110..2048
                     /locus_tag="P5079_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY28681.1"
                     /translation="MIDVKKTMAVAARFSLGLLLALLSLEFSLWATGELWFRYQRSLY
                     PAINTSQEGIRIMFLGESTTHGYYVGPEKAFPRQLEKRLNEKYKGRRYVSINLGIMAI
                     TTAQILRDFKLNMLRYRPQIVVLQAGHNQNAPGVLRALAGEITDPRVATIWMYINSTR
                     TMRLLTLFYDYGFSLLKGSALLTNEEAAGRPKIRIWGNLSGFALTKAETEANLKAIIH
                     ACVKKGVPLVLCDYFDSDANPLLRRLSAEYGLPFCENEMPPEKMTKEFKREYLWSDQW
                     HPSERGYARMAMNLLRTMEESGLVPYGASPSKADGK"
     gene            2510..3337
                     /gene="rpsB"
                     /locus_tag="P5079_01445"
     CDS             2510..3337
                     /gene="rpsB"
                     /locus_tag="P5079_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY28682.1"
                     /translation="MASISMKALLEAGVHFGHQTRRWNPKMAKYIFGSRNNIHIIDLQ
                     KTVKELKKALTYVRDMAASGQEVLFIGTKKQAKEAITAEAQRCGSPYVRDRWLGGTLT
                     NFETVRRSAKRLQELDRMKQSGIFSALSKKEGIRREKEIRRLTKSLDGIKNMEQLPGA
                     VFIVDPVQELTSIMEARRMEVPVVAICDTNCDPDLIDYPIPGNDDAIRAVRLFCKLVA
                     DSVVEGKDLAKKQAAAAAGPEAAATETEIPLTGAEPAAEAPAAPAPAEAKAENPATN"
     gene            3377..4246
                     /gene="tsf"
                     /locus_tag="P5079_01450"
     CDS             3377..4246
                     /gene="tsf"
                     /locus_tag="P5079_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRY28683.1"
                     /translation="MAEITSEAVQKLRAQTGAGIMDCKRALASAGGDMEKAVKTLREQ
                     GAASAAKKAGRVTGDGLVTSYLDPLGKTAVLLELNCETDFVARTEDFIKLIADLARRA
                     GTASPAWKGVEDGPGDLMQQMIGKLGENLTFRRFVRYDKTNPGVFGVYIHPSGGTGKV
                     GVLLELSVADPKMETNEEVKTLARDLAMQIAAANPRWVKKEDVPAEMLEQEKAIAREQ
                     ARRENKPEKIWDKIAQGKLQQFYQQFCLLEQPYVKDAGGKTPVGAVVQQLSQKTGVVL
                     SPARFVRYKVGEE"
     gene            4243..4968
                     /gene="pyrH"
                     /locus_tag="P5079_01455"
     CDS             4243..4968
                     /gene="pyrH"
                     /locus_tag="P5079_01455"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRY28684.1"
                     /translation="MSESVRGNPKRVVLKLSGEAFMGRSRSGIDVDALFAIAHEVKSA
                     RGPRTQIAVVVGGGNIWRGGRGQARKLDRMISDNMGMLATVINALALQDALEQIGVPT
                     RVLSAIEVAKVAEPYIRRRAIRHLEKGRVVIFAAGTGNPYFSTDTAAALRASEIEAQC
                     VLKATLVDGVYDSDPQKNPRATRFKTISLLEALRKRLGIMDATAMQLCLENALPVRVF
                     DLHGRGNIRRAIGGGRIGTLITP"
     gene            5013..5591
                     /gene="frr"
                     /locus_tag="P5079_01460"
     CDS             5013..5591
                     /gene="frr"
                     /locus_tag="P5079_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRY28685.1"
                     /translation="MGIPNVPQLQPVLAQMEDKMKKAVEKMRQDFSTLRTGRATGALL
                     DNLKVLYYGSQVPLKQVAAVGVPDGRTIEIKPWDVSALPEIEKAIQASDLGLTPNNDG
                     KIIRLSVPSLTEERRRDLVKAVKKMAEDFRVSIRNDRREAMEKLKKFEKDKVLSEDQR
                     KLAEEGLQKTTDLYIKQIDESLAVKEKEIMEV"
     gene            5658..6389
                     /locus_tag="P5079_01465"
     CDS             5658..6389
                     /locus_tag="P5079_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRY28686.1"
                     /translation="MSLKADELLKRIDPRRLPRHVAVIMDGNGRWAKKRGLPRLMGHR
                     AGAESVREMVRVCGDLGIEVLTLYAFSTENWLRPAAEVKGLMTLLVKTLRRETADLDR
                     NNVRLRAIGRLDKLPAEVQRELHQAMSALADNSGLILNLALNYGSRQEIVDSVNALLR
                     EGVTAVEEKDISRRLYTAGLPDPDLLIRTSGEYRLSNFLLWQCAYTELYVCSHYWPEF
                     RRAQFYEALLNYQSRERRFGGLGGT"
     gene            6430..7278
                     /locus_tag="P5079_01470"
     CDS             6430..7278
                     /locus_tag="P5079_01470"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRY28687.1"
                     /translation="MMVLPRLVTSLVAAPLFLWALYLGSLPFVVFVSVLVLLALWEFH
                     RMAEIGGYATQSWWGIAGGMMLLLALCFPGLRADLPFQNQAPALTLMVVVAAYTLREL
                     ARRDKSLSMLRLSMSLLGTFFVVWPLAHLVLLRELRHEPLYTVGRDAAFYLVFLIWVQ
                     DITAWAVGLSVGKRRLAVQISPKKSWEGAAAGLAAAVLLSLFLREVWLKTIFSRTEIV
                     ALSAALGILAQVSDLAESLMKRCFGVKDSSDLLPGHGGILDRFDSFLLAAPFLYYYLV
                     LTGRVG"
     gene            7283..8533
                     /gene="dxr"
                     /locus_tag="P5079_01475"
     CDS             7283..8533
                     /gene="dxr"
                     /locus_tag="P5079_01475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRY28688.1"
                     /translation="MHGRRISILGSTGSIGLNALEVIRKLGRFSGGGFRVWGLAAQRN
                     VPLLKKQVLEFEPRAVVVYEADAAAEMLAWARRRRLRLSVWQGVEGLERLARAPETDF
                     LLSSVVGAVGLLPLLSAVRAGKRIALANKEALIVAGDLLMESARRSGAELIPVDSEHS
                     AIFQCLQGYAAGRRSQNGESPREIRRLILTASGGAFYRRKGSLDGVSVAEALSHPTWK
                     MGNKITIDSATLTNKGLEAIEAHHLFGVPLEKIGIVIHPQSIIHSLVEFTDGAMLAQL
                     SHPDMRLPIQYALTHPARYPSLVRPLRLEEIQKLEFYPPDFRRFPCLSLALTAGRRGG
                     TWPAVFNGANEAAVRSFLDGRISFGGVPRVIGKVMRAFAAEPGLTRNGMRRPPSLADI
                     LAADAWARKKSACLCEKERKTCRS"
     gene            8521..9633
                     /locus_tag="P5079_01480"
     CDS             8521..9633
                     /locus_tag="P5079_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRY28689.1"
                     /translation="MSFLIGALGIVVVFGVVIFIHEFGHFVVAKRANVKVERFSFGLG
                     PEMVGVQWGETRYCLAWIPLGGEVRMAGETEYEEGDKAPSAPPDPRSFFAQSWQRRIG
                     IVLAGPLMNYLLAFVLFASVAYLWGEPRPSHEPVIGELMEGYPAQQAGLLPGDRIVAV
                     AGAKIARWEDLAGEIHRFPGRPIALEFVRDSETKTVQLTPKADPVTRIGLIGIAPETV
                     YHKVAFFPALRRGAEQIVGWSLFTVHYLGLKIVRREKPDLAGPIGIATVVAKATKAGL
                     KDFLYLIALISVGIGLFNLFPIPMLDGGHAVFFLWEGIFRKPLHRKIMERANAVGLAL
                     LLGILLFATYSDIQRLRPEKEPVPADGEAVHAVPRP"
     gene            9682..11415
                     /locus_tag="P5079_01485"
     CDS             9682..11415
                     /locus_tag="P5079_01485"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRY28690.1"
                     /translation="MRLSKYLLPTLKELPADADTVSAKLMLRAGLIRKVAAGLYEWLP
                     FGLRVLKKVEQIIREEMDRAGGQEVWLPIVQPKELWEETGRWQVYGKELLRIKDRKDT
                     EFCLAPTAEEVITDLVRREVRSYRELPLLLYQFGIKFRDEIRPRFGVMRAREFYMKDA
                     YSFHADDADLEKTYKEVYDAYVRICERCGFKFRPVEAATGAIGGRFSHEFMVLAETGE
                     ETIATCDVCGYAANIELAESTAFGSKKGEPLQALGEVDTPDCWTVEDVSKLLKIPKIK
                     FIKTLFYDTDQGPVVALMRGDHELNEAKLQRHLGAKWIQKASDETYRSVAHCEVGYAG
                     PVGLSARVVADWNVATVENGVTGANKKDKHLVNVNLERDCTLAPESFGDLRMVREGDP
                     CTRCFRKNTEKPTFHKLAFHKGIEVGHVFKLGTKYSESMSAGFLTEKGTKSPFVMGCY
                     GIGVSRIVAAAIEQCHDDNGILWPAPLAPFQVIVLPLNVSEPNVMAAAEKIYSELRSR
                     GVEALLDDRDQRAGVKFKDADLLGIPWRVTVGEKKLAGGCVELKSRSASASEDAPVEE
                     AADRVLKKITP"
     gene            11484..11936
                     /locus_tag="P5079_01490"
     CDS             11484..11936
                     /locus_tag="P5079_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY28691.1"
                     /translation="MPFDDTVFLKSKVDVLSFFTDEQLRRVTSVIDRKTYNKGQTVMF
                     QGEVTNNFFVVKKGKVMVVAKVGKEKERVELAELKAGDFFGEMSLLESTAATATIKSV
                     EEGTEILMIPHDSFQYLLKENPLLEKTLRDRIAARKQQKSDASPKEGA"
     gene            11979..14765
                     /locus_tag="P5079_01495"
     CDS             11979..14765
                     /locus_tag="P5079_01495"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRY28692.1"
                     /translation="MKKGWLVDTLIGLLLTLTVMACAYFQVAFTESLENRLYDLRVKY
                     APKASLSDQILFVAIDDTSIASVGRWPWPRGVVGQLVRQIAAGGPKVIGLNILYVDPD
                     QNQGLETIRQIREQYSQLIDQQKEVLGPVFTSANKDPKYKKILKGQKLSLDPFRSLVG
                     AFDEAEAVLDNDTQLAQALTETPGVLLPMFFSVDKPLAAETDEAMERMQGYALPQVGE
                     SGRPHSILEGYQPLLPLPEFAAGASGLGHSNIFADSDGTVRRETLLVKYKDKYFPSLS
                     LEMARVALGLNPDQVQASLGSAVLLGDRKIPIGQDNKMLVNYQGTYASLKTVPAIDIL
                     AEKVPAEAFKNKLVLVGISATGLGTLFVVPVESAFPANGIIVTVLQNILGGKFLTRPD
                     WSFMAELGVLLFVGLFVTFLLPRLKAKWGALIAFVLLAAIAGAGVYFFVTQGYWIKIF
                     YGMALLVLAYGAITVRRFFFTERRKELVEAESIETNKMLGLSFQGQGMLDLAFEKFRK
                     CPVDDAMKELLYNLALDFERKRQFAKASVVYEHISTVDAGYKDIKERKVATKTAGETM
                     IAGPGLGGGKKEGTVVIEGAAVKPTLGRYEIQKELGRGAMGIVYLGKDPKINRMVAIK
                     TLKFDDDVDESTSKQLKERFFREAESAGTLNHPNIVRIFDAGEDNEICYIAMELLDGE
                     DLKKYGEKANLLPPAQVLEYVALVAEALDYAHAAGIVHRDIKPANIMRLKDGTLRVTD
                     FGIARITASSKTQTGTVMGTPSYMSPEQVAGKKVDGRADLFSLGVMLYEMMTGLKPFE
                     GDSIATLLFRISSEPHPDPVLKASDRVTPAVKAVIDKALQKNPDNRYQRGKDLSADLK
                     ECLKNPNAVPTNALAAAVSAPPAAPVAPPAAPSSSDTVRLSSPPPAAPAPSPSDTVKM
                     PPPAPSDTIRMPPQ"
     gene            14827..15630
                     /locus_tag="P5079_01500"
     CDS             14827..15630
                     /locus_tag="P5079_01500"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRY28693.1"
                     /translation="MKFVAAGLTDPGQVRKNNEDNYWVDPDLGLLIVADGMGGHASGE
                     VASQMAVDLVKAQVANGLKTGKIPAMGEKPLHLSMRAHLLAVSLRMANEVVYEASQSR
                     AENKGMGTTIVAVLADRKSFAVAHVGDSRLYLHRGGKLRQLTRDHSVVAEQVAKGLLT
                     PEQAEQSEIKNVLTRALGVGPEVEVDVDEQSLRADDVLLLCTDGLPRMADDAVIQDVL
                     AHSTDPLEICNALIKIANERGGKDNVTVAVARAFASGILEKIVGRFKRA"
     gene            15627..16112
                     /locus_tag="P5079_01505"
     CDS             15627..16112
                     /locus_tag="P5079_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28694.1"
                     /translation="MIRHRIIFYLLVCSLAAFPTDFLRAQGRKAAVENTRIQTADYEG
                     VVFAAENRAFAFVFPPPAVAWTPTPEDITRVEELMADHVRRKAPGIFEKLRRYKRQYA
                     GYLQAGKKVVYVNCFCRVRGDVDWEKTELLVADGGPDFFHVTVDLSGGACYDFVVNGS
                     A"
     gene            16140..16853
                     /locus_tag="P5079_01510"
     CDS             16140..16853
                     /locus_tag="P5079_01510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY28695.1"
                     /translation="MVKLLLKFNAAVIKEIPFEKDEYTVGRKPENDIVIDNPAVSGRH
                     ARIFRQAGAYVVEDLNSTNGTFLHDRKILKANLHHKDEVGIAKHSVVFINEEEAKSPP
                     PASLPAVSSDATVVISAMAPAAAKAEAKEKELVGGLRVVTGESSAGQFSLTGLTTYIG
                     KSEQANIKLKGFFAPDLAACVARKSDGYYLKVIKERSVKLNGNVLEDQAKLQEGDLVE
                     VANLKLVFYHQDAEGPLSG"
     gene            16973..18670
                     /locus_tag="P5079_01515"
     CDS             16973..18670
                     /locus_tag="P5079_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY28696.1"
                     /translation="MIKKFLPGFALASVLAVFCSGRVSAAELRSTLGTVEVKKAGAAV
                     WSQARPKTKVNDGDVVRTGAGAQTELRFDEGHKMTLREKTTLKVITGQSPETKLDLVV
                     GRIRTFVSKLKAQRKFEVSTPVAVASIRGTIFEMEVTENQASRLSVLEGVVSYRDLAG
                     LGAEVQVLKGQSVVVEPGMAPRPPEPLPQDLREGLVPDREQQTASFDMKAEIQRETGL
                     AAYKEFFQSDAAAEMKTAQYQEGKTLIDAFGKRVRLEEYILRPAANQWSFVALNTRED
                     RFDFTRFDLYAKNALPEDMATVNLFSGTGHNSMTNWVEKTNRLTSNGSDYLREWQDGG
                     APINMTKTDGTKYQQVVFADWFVEAKAAGASAPVLLSHWQPHAGFSGGNPLATFVDAD
                     NNTVDDDVDLDGVLDIPSGYVNYESADAGVTVGDTRGIDAAGFSNSKRLDYFADAAVA
                     ASYKGSEGYARKAETLFNDKTWAASSSLEILSSNKISQLNTYTVPGQSAITLQVDQYY
                     FNDDGDRLSFLQAASLTSDSIQGINYESVATSSLYGDRKLDVVISPSIFKKAGLLNN"
     gene            18672..19892
                     /locus_tag="P5079_01520"
     CDS             18672..19892
                     /locus_tag="P5079_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28697.1"
                     /translation="MNMRRISRTVIFAAFCGVVAASASAEFRWVPYLNLESYLGRSNF
                     ADSDVNGFNGNLVFVPGMRFGEKLSVLPTAMLGYRKSRDVQELAGGGFLTQEQQTRGL
                     SVKGIYVLTPGWKGKLYTSYRQELVKETKDEKWGDGLFDYDKTAVGVEVEREGDFWKS
                     VRLGADYYTTAFPNFQSLATKQYGAEINAGEDVLDFSAMDFSVGADAAVTRKSMLSGY
                     LLGSRRDFSDQKIVKRDGTFSADTRKDMFTYVSLAYRYQLPTVALFHVGVESLAGLDA
                     SYALLNSDQHNYDAGRTKFNENYYDYAELGIGPRVNLRFKEKLSVGLSYLFSTRDYAD
                     RPVQRVDGTYTADTISSDVTTFRASLGYPLLKGLSLQAQGAWQNADSNMDYETVYRYN
                     YSSSHYFLGISYQL"
     gene            20104..20586
                     /gene="rimP"
                     /locus_tag="P5079_01525"
     CDS             20104..20586
                     /gene="rimP"
                     /locus_tag="P5079_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRY28698.1"
                     /translation="MVDEVRTLVSPVLAQEGVELVEVEYQREPNGWTLRFYLDKAGGF
                     SLADCEQWNDRLGEVLDRSELISHAYSLEISSPGLNRPLRKREDFERFSGMDAFVNLY
                     AQQNGQKHFHGRLVRVENGELVLEDRTSGFVKIPLSSIAGARLDPPVALGGNAEKDMS
                     "
     gene            20583..22061
                     /gene="nusA"
                     /locus_tag="P5079_01530"
     CDS             20583..22061
                     /gene="nusA"
                     /locus_tag="P5079_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRY28699.1"
                     /translation="MTQILKNELMPVLEQIERDKGIKKEDILHMIEQALVSAYRKHVG
                     QQVNVEAKIDPESGQIKASVVKTIVETVTNPVIEISVAEAKKVRGAGDVGSEFKIPVD
                     AAEFARIAAQTAKQVLVQKIRETERETVFSEYKPKEGTLINGAVHRFSNRNLIVDLGR
                     SEGILPVREQVRRERWTVGDRIRAVILKVEKGPRGPEIILSRAHADFVKRLFEQEVPE
                     IYEKTVEVVEVVREAGFRSKVVVRSNNPKVDPIGACVGVKGSRVRPIINELQGERIDL
                     VAYTPDTTAFIAASLSPVKPLSVTVISHEEKKAEVLVSDDQLSLAIGKNGQNVRLASR
                     LTGWQIDVRSESQKKEANAADNAAHISALSQLEGVGPKTADVLRKGGWGDISRLAGAK
                     PDDLTALSGIGEKTAEKIIESAKEYLKKKAEEEAAAPSTPPAEPEAVSPEPAAEKEAK
                     ETEEPAAEAAPAGESAAPAEKPAAEGKKSKRKQEQGTEQKDD"
     gene            22054..24222
                     /gene="infB"
                     /locus_tag="P5079_01535"
     CDS             22054..24222
                     /gene="infB"
                     /locus_tag="P5079_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRY28700.1"
                     /translation="MTDEKKTKKAPAAKKAASPKASKEAVALEDGKKPTKPKTSAKAV
                     KPKKAVDTSAPATAEPKAAKPKKKAVAAEAAAAPAEVPAKKVPAKKVKAKAPAVETKE
                     PASPPAAKISSAPAVPPKPAPAAPPKPAAVVPPAPPAPPRPKIKVNEMVTVKELADKM
                     NVKVPDVLKKFMSLGKIVHINQRVDVALAGKIADSFQFDLEVVPTYADEVLDSPDDPS
                     LLRSRPPVVTVMGHVDHGKTSLLDAIRKTRVAEKEAGGITQHIGAYQVKTEKGYITFL
                     DTPGHEAFTAMRARGAQATDLVILVVAADDGVMPQTVEAIDHAQAAGVPIVVAINKCD
                     LPTANPQRIKQELTKHKLIAEDWGGKTVMVEVSARQNKNLDTLLEMILLESELLELKA
                     NPNRPAQGVVVEAKLDPRRGAVATVLVQKGTLRVGDAFVCGLAAGKVRALLDYAGQRG
                     KEAPPSCPVEILGFSTTPRAGDKFMVVASEREAREIAERRQAIASDAAHSRRQHLTLE
                     GLHDEVAAGKIKFLPIVLKADVQGSLEPVRDSLEKLATSEISLKIIHSGIGGINASDV
                     ILAAASDAVIFGFNVRPDPSSEELARREGVEIKTYRIIYEMVNVVKAALEGLLSPAEQ
                     EVTSGWAEVRQVFRVPKAGTVAGCYVTDGRIVRSAKVRLVRDGAIVFDGAIANLKRFK
                     EDVREVEKGFECGLSLEGFQDVKPQDRIEFYIIEKKARKL"
     gene            24331..24807
                     /gene="rbfA"
                     /locus_tag="P5079_01540"
     CDS             24331..24807
                     /gene="rbfA"
                     /locus_tag="P5079_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRY28701.1"
                     /translation="MWSRRIERINELLLQEISRVVVERQDPDIGFVTFTGVEVTEDLM
                     QAKVFYSVLGSEDDRLRTAGALEHMRHEIHQDMRRLESLKRIPTLVFIHDQTPQKAAR
                     VFELIEQIHQESQPVPPEPEKKPVVRPASRGRKPASPSALKKEKKSARPPQRKKPR"
     gene            24773..25771
                     /locus_tag="P5079_01545"
     CDS             24773..25771
                     /locus_tag="P5079_01545"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRY28702.1"
                     /translation="MPARLSERNRDKIITRILRTVRENRTFFLAGHEKPDGDTIGSEL
                     ALASLLRRWKKQADIYNSEPVPKNLRFLPGAEAIRSARRVDKAYDVAIILECSGPERM
                     GNIIDLKSQAKTVINIDHHAHHATFGHINLIDAQASSNSELLYYFFQHAELKLSRDEA
                     MCLYVGLVTDTGRFQQVNTNARSHEVAAGLHRVGIDVSDITRRLYNTRTESSLKLLGR
                     AMGSLRLEFQGRVAVISLTQRDFIETSSGPEETEEFINYGLMVPSVQVALFVRETEIA
                     GKMKVSFRGKGLVDVLRVAVAYGGGGHKNAAGCTVDGNLENTVQSVLSRLQKILPA"
     gene            25783..26544
                     /gene="truB"
                     /locus_tag="P5079_01550"
     CDS             25783..26544
                     /gene="truB"
                     /locus_tag="P5079_01550"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRY28703.1"
                     /translation="MSDHPHPFFSGVLLLDKPSGPTSHDAVLWARRALHTKKIGHCGA
                     LDPLAQGLLLLAMGEALEWQERLMRQRKVYRGSFRLGVVTDTDDITGQILRQETDARR
                     VAAVSDEALAGLFKRFLGPIRQRVPRYSSVKVNGRRLHEWARRGVEMPLPEKTVEISR
                     FDLLSYAPPDAHFVVECSKGTYVRSLARDVGEQLGLGATLASLCRESIGRFSRADAFA
                     WNGDFSMEPTDWERAFIPMDELPALFPAGEPAAVR"
     gene            26541..27452
                     /gene="ribF"
                     /locus_tag="P5079_01555"
     CDS             26541..27452
                     /gene="ribF"
                     /locus_tag="P5079_01555"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRY28704.1"
                     /translation="MSAPRRGTVLTIGTFDGVHVGHRVILRKAVSLARKAGLSSLALT
                     FARPPRLYFSPQKGPLLITLPEEKAELLRSIGVDRVEFLAFGKKLAALSPEDFFKEHL
                     LRRYGARQVVVGFNFGFGRRRAGHLGLLKQLGASHGVPVHIVPQVSLGGRRVSSGKIR
                     RDLWRGELAAANRKLGYPYFLSGQVVRGDRLGRRLGFPTANVRVPDEKLVPPGVFAVR
                     VVLPNGTEKPGMCNVGFRPTLGRRAPRLSIEAHVLDFRGNLRGKKIKVVFVKKIRSEK
                     AFPTLEKLAARLRIDAAVARRILRRSA"
     gene            27449..>28134
                     /locus_tag="P5079_01560"
     CDS             27449..>28134
                     /locus_tag="P5079_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28705.1"
                     /translation="MTRRLAVCLLAGLAASSVFAAQELPHFIASRHFSVQYESPYPPA
                     GVLNTLEGLHAKLLLDLLPFAPWAKDGKIQVVLYSSAERYRAVEVMPAWAGGHVDVKA
                     RRIFLFEGDEFRRTMAHEMAHLFVQDYFAARGSVPPDWLNEGVAMRMEGDYGGAVSSG
                     GRELKEASPSLEAFFNLNYRHAAQRPEEVALWYGQAGSLAGYLMRQFPRGQFVAFCDR
                     LRNGASVTLEP"
BASE COUNT         5464 a         8728 c         8921 g         5021 t
ORIGIN      
        1 cggggagttg gcttttctcg gggcgccgga actcgtccgc ctcctgtccg acgcctcgcg
       61 ggcgatgtgc gagggggaga tcggccagat cgagaggcgc ttccatttcg acctgtcgga
      121 ggaggaatac ctttcgttcc tcgacaaaaa gaccgcttcc ctgatggcgg cctcctgccg
      181 ggccggcggc gcggcggccg gcctctcccg gaaacaacag gaggcgttgg aaacgttcgg
      241 ccggcggctg ggcgtggctt ttcagatcgt ggacgacatc ctcgacctgg aaggagacga
      301 ggcggtgacc gggaagaccc tgcgcaccga cctgcagcac ggcaagatga ccttgccgct
      361 catccgctac cgcgaccgtc tggggagtcg gtccgaaaga aaaaagtttt gtgaaagtct
      421 gcgggacggg gcgggcggct tttcgggact cattgggaag atccgggacg cgggggccat
      481 agccgagagc cgggccaagg cgcggttgtt tgtggacgag gccctgcgcg cactggacga
      541 attgcccggt catcccgccc ggcaaatttt aagcgacgtc gctcaccgtc tcacccttcg
      601 ccgattttga tggttcgggg aggcggaact gaaaaatccc ggcaggcgtt tctggcctcc
      661 cgggattttt tgacggggaa tgggggggga ggctatttcg gcgtgatctt gatcgccacc
      721 accgggcaca cggcctcgca ggccatgcag aagatgcagg cgtcctcttt ggccgggtcg
      781 gcttttttgg cggaggcggc gtggcccggg gtgtccagcc attcgaacac gttttccggg
      841 caggcgtcga ggcaggcgcc gtccgcgata cagaggtcga aatccaccgc cacccacgtg
      901 ccgtgaatcc ccagtttggc gggtttttcc tcgggccccc aaacggcgtg gcccgcatgc
      961 tcgcctactt ttgttcggtt tttctggaat tcgggatcga tggcagtcat cgttttgccc
     1021 ccctttgccg ggttgaatgt ccttcttcta aaaatctacc ataaggggct ccggcccgtc
     1081 aaacctcaga aaggaggtcc cgcccgcacg tgatcgacgt caaaaaaacg atggccgtgg
     1141 ccgcccggtt ttcgctggga ctgctcctcg cgctcctgtc cctggaattt tcactgtggg
     1201 cgacgggaga gctgtggttt cgttatcagc ggtctttgta tcccgccatc aacacttctc
     1261 aagagggcat ccgaatcatg tttttgggtg aatcgacgac gcacggatac tatgtggggc
     1321 ccgaaaaagc atttccccgt cagttggaga aacggctcaa tgaaaaatac aagggacggc
     1381 gatatgtctc gatcaacctc ggtataatgg caataaccac cgctcagatt ttaagagact
     1441 tcaaattgaa tatgttgaga taccggccgc agatcgtcgt gctccaggcc ggacacaatc
     1501 agaacgctcc cggtgttctg agggcgctgg ccggcgaaat cacggacccg cgcgtggcga
     1561 caatttggat gtatattaat tccacgagga cgatgcggtt gttgactctt ttctatgact
     1621 acggtttttc actgctgaag ggctctgctc ttctgaccaa cgaagaagcg gcggggcggc
     1681 cgaaaattcg gatatggggg aacctctcgg gtttcgcatt gaccaaagcc gaaaccgagg
     1741 ccaatctgaa agccatcatc catgcgtgcg tcaaaaaagg cgtgccgttg gtcttgtgcg
     1801 attatttcga ttccgacgcg aatccgcttc tgcggcggct gtccgccgaa tacggactcc
     1861 ctttctgtga gaacgagatg ccgcccgaaa aaatgacgaa ggagttcaag cgggaatatc
     1921 tctggtccga tcaatggcat ccgagcgaga ggggctatgc ccgtatggca atgaatttat
     1981 tgcgcaccat ggaagagagc gggctcgtcc cttacggcgc ttccccgtca aaggccgacg
     2041 gaaaatgacc ggttccgaac aaccgggggg gcgcgcccgg atcgcccgga acctgccgcg
     2101 gccgctggaa aagagcggcc gggtccccgg tcatgcggaa gtccccggca atcttgcgga
     2161 gcgcgctcca aaaaggatat aatccccgtg accgtgcgcc ttcgggacgt cttccgggcg
     2221 ccaattcgca cacctttcgg attccggccg gggtctccgg tccagtcgga tcggagccgg
     2281 ttcgggagtg gatgaagggt ggaggtcgcc ccgctcgacg gggtgaataa aaccagacac
     2341 aggttgttcg ccggtggttc gaaatgggaa atcccgcgga gcgtcaaaag ccgcgggaac
     2401 tgtgctgggc ggtcgctttg cggcgaccgc tttttttctg actcgaacat cggcggatga
     2461 gggcccaacg gccccggacc ggaaacggtc caccgaacta ggaggaacaa tggcatcaat
     2521 ctcaatgaag gcacttctgg aggcgggggt tcatttcggc caccagacgc gccgttggaa
     2581 tccgaaaatg gccaagtaca ttttcgggag ccgcaacaac atccacatca tcgacctgca
     2641 gaaaaccgtc aaagaactca aaaaagccct cacctatgtc cgcgacatgg cggccagcgg
     2701 tcaggaggtg ctcttcatcg gcaccaagaa acaggcgaaa gaggccatca ccgcggaggc
     2761 ccagcggtgc ggctcccctt acgtgcggga ccgttggctg ggagggacgc tcaccaattt
     2821 tgagacggtg cgtcgttcgg cgaagaggtt gcaggaattg gaccgcatga aacagagcgg
     2881 tattttctcg gccctctcca agaaggaggg gatccgccgc gagaaggaga tccgccggct
     2941 caccaagtcg ctcgacggca tcaagaacat ggagcagctt ccgggcgcgg tgttcatcgt
     3001 ggaccccgtc caggagctca cctccatcat ggaggcccgc cgcatggagg tccccgtggt
     3061 ggcgatctgc gacaccaact gcgatccgga cctcatcgat tatcccatcc ccggcaacga
     3121 cgacgccatc cgggcggtcc gcctgttctg taaattggtg gcggacagcg tggtggaggg
     3181 gaaggacctc gccaagaaac aggcggcggc agccgccggg ccggaagcgg cggccacgga
     3241 gacggagatc ccgctgacgg gggcggagcc ggcggccgaa gcgcccgccg ctcccgcgcc
     3301 cgccgaagcg aaggcggaga acccggccac caactgaaac atcgcgtcat cacattctga
     3361 ggaggatttt tcacccatgg cggaaattac gagtgaagcc gtacagaaac tgagggccca
     3421 gacgggcgcc ggcatcatgg actgcaaaag ggccttggcg tcggccgggg gggacatgga
     3481 gaaggccgtc aagaccttgc gcgaacaggg ggcggcctcc gcggctaaaa aagcggggcg
     3541 cgtcaccggc gacgggctgg tgacgtccta cctggatccg ttggggaaga ccgcggtgct
     3601 gctggagctg aactgcgaga cggatttcgt cgcccgcacg gaggatttca tcaagctgat
     3661 cgcggacctc gcccgccggg ccggcaccgc ctcgcccgcc tggaagggcg tcgaagacgg
     3721 ccccggcgac ctcatgcagc agatgatcgg caagctcggc gagaacctca cgttccgtcg
     3781 gttcgtccgc tacgacaaaa cgaatccggg ggttttcggc gtctacatcc accccagcgg
     3841 cggcaccggc aaggtggggg tgttgctgga gctctccgtg gccgacccga agatggaaac
     3901 caacgaggag gtgaagaccc tggcgcgcga cctcgccatg cagatcgcgg ccgccaaccc
     3961 tcgctgggtc aaaaaagagg acgtgccggc ggagatgctc gaacaggaaa aggccatcgc
     4021 ccgggagcag gcgcgccggg agaacaagcc ggaaaaaata tgggataaga tcgcccaggg
     4081 caaactgcag cagttctacc agcagttctg cctgctggag cagccttacg tgaaggacgc
     4141 cggcggcaag acccccgtgg gcgccgtggt ccagcaactc tcgcaaaaga ccggggtcgt
     4201 cctctcgccg gcgcgtttcg ttcgctacaa ggtcggggaa gagtgagcga gtcggtgcgc
     4261 gggaacccga agcgggtcgt actgaaactg tccggggagg ctttcatggg ccgttcccgg
     4321 tccggcatcg atgtggacgc gctcttcgcc atcgcccacg aggtgaagtc cgcccgcggg
     4381 ccccggaccc agatcgcggt ggtggtgggc ggggggaaca tctggcgcgg cgggcgaggc
     4441 caggcgcgta aactcgaccg catgatctcc gacaacatgg gcatgctcgc caccgtcatc
     4501 aacgcgctgg ctctgcagga cgcgctggaa cagatcggcg ttcccacgcg cgttctttcg
     4561 gccatcgagg tggccaaggt ggccgaaccg tacatccgcc gccgcgccat ccggcatctg
     4621 gagaaaggcc gggtggtcat cttcgccgcc ggcaccggca acccctactt ctccaccgac
     4681 accgccgccg ccctgcgcgc ctctgaaatc gaggcccagt gcgtgctcaa ggccacgctg
     4741 gtggacggcg tttatgattc cgatcctcaa aagaaccccc gggccacgcg gttcaagacc
     4801 atctccctcc tggaggcgtt gcggaaacgg ttgggcatta tggacgccac cgcgatgcag
     4861 ctctgcctgg agaacgccct cccggtgcgg gtgttcgatt tgcacggccg gggcaacatc
     4921 cggcgggcca tcggcggcgg ccggatcggc accctgatca cgccgtaagg cgatcgactt
     4981 ttaaactttt ccgacaaggg aggtaggcgc tcatgggcat ccccaacgtt cctcagcttc
     5041 aaccggttct ggctcagatg gaagacaaga tgaaaaaagc cgtggaaaag atgcggcagg
     5101 atttttccac cctccggacg ggccgcgcca ccggcgcgtt gctcgacaac ctcaaggtgc
     5161 tctactacgg ctcgcaggtg cccctcaaac aggtggccgc cgtgggcgtg ccggacggcc
     5221 gcaccatcga aataaaaccg tgggacgtct cggccctccc ggagatcgag aaagccatcc
     5281 aggcatccga cctgggcctc accccgaaca acgacggaaa gatcatccgg ttgtccgtgc
     5341 cctcgttgac cgaggagcgc cgccgggacc tggtgaaggc cgtcaagaaa atggccgagg
     5401 atttccgcgt ttccatacgc aacgaccgcc gcgaagccat ggagaagttg aagaaattcg
     5461 agaaggacaa ggtcctttcg gaagaccagc gcaagcttgc cgaagagggg ctccagaaga
     5521 cgacggactt gtacatcaaa cagatcgatg aatccctggc ggtgaaggaa aaggagatca
     5581 tggaggtttg agccggattt gatgatggtt tttcgcgtca caacgtcttt tttccacggt
     5641 cttgttccga acacctcatg tctttgaaag ccgacgaact cctgaaacgg atcgatccgc
     5701 gtcgcctgcc ccggcacgtg gccgtgatca tggacggcaa cggccgctgg gcgaagaaac
     5761 gcggcctgcc ccgcctgatg ggacatcgcg ccggcgccga aagcgtgcgc gagatggtga
     5821 gggtgtgcgg ggatctggga atcgaagtcc tcaccctcta tgcgttctca acggagaact
     5881 ggctgcgtcc cgccgccgag gtgaaaggcc tgatgacgct gctggtgaag acgttgcgac
     5941 gcgagacggc cgacctggac cgcaacaacg tccgcctgcg ggccatcggc cggctggaca
     6001 agctgccggc ggaggtccag cgtgaacttc accaggccat gtcggcgctg gcggacaact
     6061 ccgggctcat cttgaatctg gcgctcaact acggcagccg ccaggagatc gtggactcgg
     6121 tcaacgccct tcttcgggag ggcgtcacgg cggtggaaga gaaagacatt tcccgccggc
     6181 tctataccgc cggcctgccg gacccggacc tgctgatccg aacgtcgggc gaataccggc
     6241 tctccaattt cttgctctgg caatgcgcct acacggaact ctatgtctgt tcccactatt
     6301 ggccggaatt ccgtcgggcc cagttttacg aggctctcct taattatcag tcgcgtgaac
     6361 gccgcttcgg cggtttgggc ggaacatgaa ctccccgatg gggccgttgg gaaaagaggg
     6421 aaaaacagca tgatggtatt gccgcgtttg gtgacgagtc tggtggcggc cccgctcttt
     6481 ttgtgggcgc tctatctggg cagtctgccg ttcgtcgttt ttgtgtccgt gttggtgctt
     6541 ttggcgctgt gggagttcca ccggatggcg gagatcggcg ggtacgccac ccagtcctgg
     6601 tggggcatcg cgggcggcat gatgctcctg ttggccctct gtttccccgg cctgcgcgcg
     6661 gacctgccgt tccagaacca ggccccggcg ctgacgctga tggtggtggt ggcagcctac
     6721 accctccgcg aattggcccg gcgggacaag agcctctcca tgctgcgttt gagcatgtcg
     6781 ttgctcggca ccttcttcgt cgtctggccg ctggcccacc tggtgctgtt gcgtgagttg
     6841 cgacacgagc ccttgtacac cgtgggccgc gacgccgcct tctatctggt ctttttgata
     6901 tgggttcaag acatcaccgc gtgggcggtg ggcctttccg tcgggaaacg gcggttggcg
     6961 gtccagatca gcccgaagaa gtcctgggag ggggccgccg ccggtctggc cgcggcggtg
     7021 ctcctgtcgc tgttcctgcg ggaggtctgg ttgaagacga ttttttcacg gacggagatc
     7081 gtcgcccttt cggcggccct ggggatattg gcccaggtgt cggatttggc ggaatccctg
     7141 atgaaacgct gtttcggcgt caaggattcc tcggatctcc tcccgggcca cggcgggatc
     7201 ctcgaccggt ttgactcctt cctcctcgcc gcgccttttc tctactatta ccttgtgttg
     7261 accggccgcg tgggctgaag gcgtgcacgg tcggcggatt tccatcctcg gttccaccgg
     7321 ctccatcggt ttgaacgcgc tggaggtcat ccgcaagctc ggccgtttct ccggcggggg
     7381 gttccgcgtg tgggggctcg ccgcccagcg gaacgtgccg cttctgaaaa aacaggtttt
     7441 ggagttcgag ccccgcgcgg tggtcgttta cgaggcggat gcggcggcgg agatgctcgc
     7501 ctgggcccgt cgtcgtcggc tccggttgtc cgtgtggcag ggagtggaag ggctggagcg
     7561 gctggcccgg gccccggaaa cggattttct tttgtcctcg gtggtggggg ccgtcgggct
     7621 gttgccgctc ctttccgcgg tgcgtgccgg gaaacgcatc gccttggcca acaaggaggc
     7681 gctgatcgtg gcgggggacc tcctcatgga atccgcccgc cggtccgggg cggagctcat
     7741 cccggtggac agcgaacatt cggccatctt ccagtgcctg caggggtatg ccgccggccg
     7801 tcgctctcaa aacggggaat ccccgcggga gatccggcgg ctcatcctca ccgcgtcggg
     7861 cggcgctttc taccggcgca aggggtcgtt ggacggggtg tccgtcgccg aggcgttgtc
     7921 ccacccgacg tggaagatgg ggaacaagat caccatcgac agcgccacgc tgacgaacaa
     7981 gggcctggaa gccattgagg cgcaccatct tttcggcgtg ccgctggaga agatcggcat
     8041 cgtcatccac ccgcagtcca tcatccactc gctggtggag ttcaccgacg gggccatgct
     8101 ggcccagctg tcccatccgg acatgcgcct gccgatccaa tacgccctga cccacccggc
     8161 gcggtacccg tcgctggtgc ggccgctgcg gttggaggag atccagaaac tggaatttta
     8221 tccgccggat ttccggcgtt tcccgtgcct gtcgctggcc ctgacggccg gccggcgggg
     8281 cggcacgtgg ccggcggtct tcaacggcgc caacgaggcg gcggtccgga gtttcctcga
     8341 cgggaggatt tccttcggag gggtgccgcg ggtcatcgga aaagtgatgc gggccttcgc
     8401 ggccgagccc ggcctgaccc ggaacggaat gcgccgccct ccctccctcg ccgatatttt
     8461 ggcggcggac gcgtgggcga gaaaaaaatc agcgtgtctt tgcgagaagg agaggaaaac
     8521 atgtcgttcc tgatcggcgc cctgggaatc gtcgtcgtct tcggagttgt catcttcatc
     8581 catgagttcg gccatttcgt cgtcgccaag cgggcgaacg tcaaggtgga acgcttctct
     8641 ttcggcctcg gccccgagat ggtcggcgtt cagtgggggg aaacgcgcta ctgtctggcc
     8701 tggatcccgc tgggcggcga ggtgcggatg gcgggggaga ccgaatacga agagggcgat
     8761 aaggccccgt ccgccccgcc ggacccgcgc tccttcttcg cgcagtcgtg gcagcggcgg
     8821 atcggcatcg tcctggcggg ccccctgatg aactatcttc tcgcgtttgt cctgttcgcc
     8881 tccgtggcgt atctgtgggg cgaaccgcgg ccgtcccacg agccggtgat cggcgaattg
     8941 atggaaggct atcccgccca acaggccggc ctcctccccg gcgaccgcat cgtcgctgtg
     9001 gcgggggcga agatcgcccg gtgggaagac ctggcgggag agattcaccg ttttccgggc
     9061 cggcccatcg cgctggagtt tgtgcgcgat tcggagacca agacggtgca gctgacgccg
     9121 aaggccgacc cggtcacccg gatcggcctg atcgggatcg ctcccgaaac ggtctatcac
     9181 aaggtcgcgt tcttcccggc gcttcgccgc ggcgccgaac agatcgtcgg ctggagcctc
     9241 ttcaccgtcc attacctcgg cctcaagatc gtccgacgcg aaaaaccgga cttggcgggg
     9301 cccatcggca tcgccacggt cgtggccaag gccaccaagg cgggtttgaa ggatttcctc
     9361 tacctcatcg ccctcatttc ggtcggcatc gggctgttta atctcttccc cattcccatg
     9421 ttggacgggg gccatgcggt ctttttcctg tgggagggga tcttccggaa gccgctccat
     9481 cggaagatca tggaacgggc caacgccgtc gggctggccc tgcttctggg catcttgctt
     9541 ttcgccacgt acagcgacat ccagcgattg cgtcccgaga aagaaccggt cccggcggac
     9601 ggggaggcgg ttcatgccgt tcctcggcca taaaatgatt tcccatgccc ggccccgcgg
     9661 ccgccggtcc ccggcccgat tatgagactc tccaaatatc tcctgcccac cttgaaggag
     9721 cttcccgccg acgcggacac cgtgtcggcg aagctgatgc tccgcgccgg cctcatccga
     9781 aaggtggccg cgggccttta cgagtggctg cccttcggcc ttcgcgtgct gaaaaaagtg
     9841 gaacagatca tccgtgagga aatggaccgc gcgggggggc aggaagtctg gctccccatc
     9901 gtgcagccca aggaactgtg ggaggagacc ggccgctggc aggtctacgg caaggaactt
     9961 ttgcgcatca aggaccggaa ggacacggag ttctgcctgg ccccgacggc cgaggaggtc
    10021 atcacggacc tggtgcgccg cgaggtgcgt tcctaccgcg aactgcccct cctgctctac
    10081 cagttcggca tcaagttccg cgacgagatt cgtccccggt tcggggtgat gcgtgcccgc
    10141 gagttctata tgaaagatgc ctactccttt cacgccgacg acgccgattt ggagaagacc
    10201 tataaagagg tctacgacgc ctacgtgcgg atctgcgagc ggtgcggttt caagttccgg
    10261 ccggtggagg ccgccacggg cgccatcggc ggccgtttct cgcacgagtt catggtgctg
    10321 gccgagaccg gggaggagac catcgccacc tgcgacgtct gcgggtatgc cgcgaacatc
    10381 gagctggccg aatcgacggc gttcgggtcg aagaaggggg agccgctcca ggcgctcggt
    10441 gaggtcgata cgcccgactg ctggaccgtg gaggacgttt ccaaactcct gaaaatcccc
    10501 aagatcaagt tcatcaagac ccttttctac gacacggacc agggcccggt cgtggcgttg
    10561 atgcgcgggg atcatgaatt gaacgaggcg aaactccagc gccatctggg ggcgaaatgg
    10621 atccaaaagg cgtccgacga gacgtaccga tccgtcgccc attgcgaggt gggctacgcg
    10681 gggccggtcg gcctctccgc gcgcgtggtc gcggactgga acgtggcgac ggtcgaaaac
    10741 ggcgtgaccg gcgcgaacaa aaaggacaag cacctggtca acgtcaacct ggagcgcgat
    10801 tgtacgctgg cgccggagtc tttcggcgac ctgcgcatgg tgcgcgaggg ggatccgtgc
    10861 acgcggtgtt tccggaagaa caccgagaaa ccgacgttcc ataagctggc gttccacaag
    10921 gggatcgagg tggggcacgt tttcaagctg gggaccaaat actcggaatc catgtccgcc
    10981 ggatttttga ccgagaaagg gacgaagtcc ccgttcgtta tggggtgtta cggcatcggc
    11041 gtgagccgca tcgtggccgc ggccatcgaa cagtgccacg acgacaacgg catcctctgg
    11101 cccgcgccgc tggcgccctt ccaggtcatc gtcctgccgt tgaacgtctc cgaaccgaac
    11161 gtaatggccg cggccgagaa gatctattcc gaactgcggt cccgcggcgt ggaggcgttg
    11221 ttggacgacc gcgatcagcg cgccggcgtc aagttcaagg acgccgacct tttgggcatt
    11281 ccctggcgcg tcaccgtggg cgagaagaaa ctcgccggcg gatgtgtgga actgaaatcg
    11341 cgctccgcct ccgcctcgga ggatgcgccc gtggaagagg ccgcggaccg ggtcttgaaa
    11401 aaaataaccc cataactttt gacataaggg cggctattag gtaaattcct gaagttgagc
    11461 ccgtatccaa ggagagagaa aaagtgcctt tcgacgatac cgttttcctg aagagtaaag
    11521 tggacgtcct gtcctttttc accgacgaac agctgcgccg ggtgacgtcg gtcatcgacc
    11581 ggaagaccta caacaagggc cagacggtga tgttccaggg cgaggtgacg aacaatttct
    11641 tcgtggtgaa aaaaggcaag gtcatggtcg tggccaaggt cggcaaggag aaggagcgcg
    11701 tggagctggc cgagctgaag gccggcgact ttttcggcga gatgtccctc ctggagtcca
    11761 ccgccgccac cgccaccatc aagtccgtgg aggagggcac cgagatcctc atgatcccgc
    11821 acgactcctt ccaatacctg ctgaaggaaa acccgctttt ggaaaaaaca ttgagggata
    11881 ggatcgcggc ccgcaaacag caaaaatccg acgcctcccc aaaggaaggc gcgtaaaggc
    11941 cgccggttca agagggaaaa aaggacgaag gacatcgatt gaaaaaaggc tggttggtcg
    12001 acaccctgat cggtttgctc ctcaccctga cggtgatggc ttgcgcgtat tttcaagtgg
    12061 ccttcaccga atccctcgaa aaccggctct acgatctccg agtcaaatac gctcccaagg
    12121 cttccctttc ggaccaaatc ctcttcgtcg ccatcgacga taccagcatc gcctccgtgg
    12181 gccgctggcc gtggccgcgc ggggtggtgg gccagctcgt ccggcagatc gccgccggcg
    12241 ggccgaaggt catcggcctg aacatcctct acgtggatcc cgaccagaac cagggactgg
    12301 agaccatccg ccagattcgg gaacagtatt cccagctcat cgaccaacaa aaagaggtcc
    12361 tgggaccggt tttcaccagc gccaacaagg acccgaaata caagaaaatt ctgaaaggcc
    12421 agaagctctc cctcgatcct ttccggtccc tggtcggcgc gttcgacgaa gcggaggcgg
    12481 tgctcgacaa cgacacccag ctcgcccagg ccctgacgga aacgccgggg gtgctcctgc
    12541 cgatgttctt ctccgtggac aagccgctgg ccgccgagac ggacgaggcg atggagcgga
    12601 tgcagggcta cgcccttccc caggtggggg aatccggccg gccccactcc attctggaag
    12661 gataccagcc gctcctgccg ctgcccgagt tcgccgccgg cgcctccggc ctggggcaca
    12721 gcaacatctt cgccgattcc gacgggaccg tccggcggga gaccctgctc gtcaaataca
    12781 aggacaaata tttcccgtcg ctttcgctcg agatggcccg ggtggccctg gggctcaatc
    12841 cggaccaggt ccaggcgagt ttggggagcg cggtcctctt gggcgaccgg aagattccga
    12901 tcggccagga caacaagatg ctcgtcaatt atcagggcac ctacgcctcg ctgaagaccg
    12961 tgcccgccat cgacattttg gcggagaagg tcccggcgga ggcgttcaag aacaaactgg
    13021 tgctggtggg catcagcgcc accgggctcg gaacgctctt cgtggttccc gtggagtcgg
    13081 cttttccggc caacggcatc atcgtcacgg tgcttcagaa catcctcggc ggaaaattcc
    13141 tcactcgccc ggactggtct ttcatggccg agctgggggt cctgctgttc gtcgggttgt
    13201 tcgtcacctt cctgctgccg cggctgaagg cgaagtgggg ggcgctgatc gcttttgtcc
    13261 tgttggccgc catcgccggg gcgggcgtct atttcttcgt cacccagggg tattggatca
    13321 agatcttcta cggcatggcg ctgttggtgc tggcctacgg cgccatcacc gtgcggcggt
    13381 tcttcttcac cgagcggcgc aaggagctgg tggaggcgga gagcatcgag accaacaaga
    13441 tgctcgggct gtcctttcag gggcagggca tgttggatct ggccttcgaa aagttccgca
    13501 aatgccccgt cgacgacgcc atgaaggagc tgctgtataa cctggcgctg gacttcgagc
    13561 gcaaacgcca gttcgccaag gcctccgtgg tgtatgaaca catctccacc gtggacgccg
    13621 gttacaagga catcaaggaa cggaaggtgg ccaccaagac cgccggcgaa acgatgatcg
    13681 ccggccccgg actcggcggc ggcaaaaagg aaggcaccgt ggtcatcgag ggggccgccg
    13741 tcaaacccac gctcggccgg tacgagatac agaaggaact ggggcgcggc gccatgggca
    13801 tcgtctacct tggcaaggac cccaagatca accgcatggt ggccatcaag accttgaagt
    13861 tcgacgacga cgtggacgag tccacctcca aacagttgaa ggaacgtttc ttccgcgagg
    13921 cggagtcggc ggggaccctg aaccacccca acatcgtccg tatcttcgac gccggggagg
    13981 acaacgagat ctgctacatc gccatggaac tgctggacgg agaggacctg aagaaatacg
    14041 gggaaaaggc caacctcctc cctccggccc aggtgctgga atacgtggcc ctggtggcgg
    14101 aggcgctcga ttacgcccac gccgccggca tcgtccaccg cgacatcaaa cccgccaaca
    14161 tcatgcgcct caaagacggg actctccgcg tcaccgattt cggcatcgcc cgcatcaccg
    14221 cctcctccaa gacgcagacc ggcacggtca tgggcacccc cagctacatg tcccccgaac
    14281 aggtggcggg gaaaaaggtg gacggccgcg cggacctgtt ctccctcggc gtgatgctct
    14341 acgagatgat gaccggcctg aaaccgttcg agggagacag catcgccacc ctgcttttcc
    14401 ggatctccag cgagccgcac cccgatcccg ttctgaaggc aagcgaccgg gtcaccccgg
    14461 cggtgaaggc ggtgatcgac aaggccctgc agaaaaatcc cgacaaccgg tatcagcgcg
    14521 ggaaggatct ttccgccgat ttgaaagagt gcctgaaaaa cccgaacgcg gtccccacga
    14581 acgcgctcgc cgcggcggtg tcggcgcctc ccgcggcccc cgtcgcgccg ccggccgctc
    14641 cgagttcgtc ggataccgtc aggctttcgt ctcctccgcc ggccgccccg gctccgtctc
    14701 cctcggacac ggtgaaaatg ccgccgcccg cgccgagcga taccatccgg atgccgccac
    14761 agtgaagaaa attcgaaata cgagacgcga aatacgaagc aaatcggttg tttcgtatct
    14821 gcctttatga aattcgtcgc cgccggattg accgatcccg gacaggtccg gaagaacaac
    14881 gaagataatt attgggtgga tcccgacctc ggtctcctca tcgtggccga cggcatgggg
    14941 ggccatgcct ccggcgaggt ggccagccag atggcggtgg acctggtgaa ggcgcaggtg
    15001 gccaacgggc tcaagaccgg aaaaattccg gccatgggcg aaaaacccct gcatctttcc
    15061 atgcgggcgc acctcctggc cgtctccctg cgcatggcca acgaggtggt ctacgaggcc
    15121 tcgcagtccc gcgcggagaa caaggggatg gggaccacca tcgtcgccgt cctggcggac
    15181 aggaagagtt tcgcggtggc gcatgtgggg gacagtcggc tttatctgca tcgcgggggg
    15241 aaactccgtc agttgacgcg ggatcattcc gtcgtggcgg aacaggtcgc caaggggttg
    15301 ctcacgccgg aacaggccga gcagtccgag atcaaaaacg tgctcacccg cgcgctgggc
    15361 gtggggccgg aggtggaggt ggacgtggac gagcagtctc tccgggccga tgatgtgcta
    15421 ttattgtgca cggacggcct tccgcgcatg gccgacgatg ccgtcatcca ggacgtgctg
    15481 gcgcactcca ccgatcctct tgagatttgc aacgccctca tcaaaatcgc caacgagcgt
    15541 gggggaaaag acaatgtcac ggtcgccgtc gcccgcgcgt ttgcctccgg gattctcgaa
    15601 aaaatcgtcg ggcgctttaa acgggcttga tccgacatag gataattttt tacctgctgg
    15661 tctgttcttt ggctgcgttc ccgacggatt tcctgcgcgc gcaaggccgc aaggcggcgg
    15721 ttgaaaacac gcggatccag accgcggact acgagggcgt cgtttttgcg gcagagaaca
    15781 gggcgttcgc gtttgtgttc ccgcccccgg ccgtcgcctg gaccccgacc ccggaagaca
    15841 tcacccgggt ggaagagctg atggcggacc atgtgagacg gaaggccccg gggatattcg
    15901 aaaagctgcg gagatataaa aggcaatacg ccggatatct tcaggcgggg aaaaaagtcg
    15961 tttatgtgaa ttgtttttgc cgtgtccggg gggacgtgga ttgggaaaaa acagagctcc
    16021 tcgtcgccga cggcggtccc gatttttttc atgtaacggt tgatttaagc gggggcgcgt
    16081 gttacgattt tgtcgtgaat ggcagtgcgt gatgataaaa ctgatccgag gacgcgaaat
    16141 tggtaaaact tctacttaaa ttcaatgccg ccgtcatcaa ggaaattcct ttcgaaaagg
    16201 acgaatacac cgtcggccgc aagccggaga acgacatcgt catagacaac ccggcggtgt
    16261 ccggccggca cgcccgcatc ttccgtcagg ccggggccta cgtggtggag gatctcaaca
    16321 gcaccaacgg caccttcctg cacgaccgaa agatcctcaa ggcgaacctg catcataagg
    16381 acgaggtggg gatcgccaag cattccgtgg tgttcatcaa cgaggaagag gcgaaaagcc
    16441 caccgccggc ctcgctgccg gcggtctctt ccgacgccac cgtggtcatt tccgcgatgg
    16501 cgcccgcggc ggccaaggcg gaagcgaagg agaaggagct cgtcggcggc ctgcgcgtcg
    16561 tcaccggcga gagttccgcc ggccagttca gcctcacggg gctgactacc tatataggga
    16621 aatcggaaca ggccaacatc aaattgaaag gtttcttcgc gcccgacctg gcggcttgcg
    16681 tggcccgtaa atcggacggt tactacctca aggtcatcaa ggaacgctcc gtcaagctca
    16741 acggcaacgt gctggaggat caggcgaaac ttcaggaggg ggacctcgtc gaagtggcca
    16801 atttgaagct ggtcttctac catcaggacg ccgagggacc gctctccggc tgatggcggc
    16861 atccccgccc catgtgacat atctcacatt gcgttccccg accgggcaag atatacttct
    16921 ctgatacgca cgatctctga ttccgaacca ttgtctccgt ggaggattcg atatgattaa
    16981 aaaattccta cctggtttcg ccctggcgtc cgtcttggcc gtgttctgct ccggacgggt
    17041 gagcgctgcc gaactgcgtt ccactttggg caccgtcgag gtgaaaaagg cgggggccgc
    17101 ggtgtggtcc caggcccgcc ccaagaccaa ggtgaacgac ggcgacgtgg tccgcacggg
    17161 cgcgggggcc cagacggagc tccgattcga cgaaggccat aagatgaccc tgcgcgagaa
    17221 gaccacgctg aaggtcatca ccggccagtc gccggagacc aagctcgacc tcgtggtcgg
    17281 ccgcattcgc acgttcgtct ccaaactcaa ggcccagcgc aaattcgagg tctccacccc
    17341 cgtggcggtg gcctccatcc gcggcacgat attcgagatg gaagtgacgg agaaccaggc
    17401 gtcccggctg tccgtcctgg aaggcgtggt gagctaccgc gatttggccg gcctcggcgc
    17461 cgaggtccag gtgcttaaag gacagagcgt ggtggtggaa cccggcatgg cgccgcgtcc
    17521 gccggaaccg cttccgcagg accttcggga aggcctcgtg cccgaccgcg aacaacagac
    17581 ggcctctttc gacatgaaag ccgagatcca gcgcgagacc ggactggcgg cgtataagga
    17641 gttcttccag tccgacgccg ccgccgagat gaagaccgcc cagtaccaag aaggcaaaac
    17701 cctcatcgac gctttcggca aacgggtgcg gttggaggaa tacatcctgc ggccggccgc
    17761 caaccaatgg tcgttcgtgg cgctgaacac ccgcgaggac cgcttcgatt tcacgcggtt
    17821 cgacctctat gccaaaaacg ccctgccgga agacatggcc accgtcaacc tcttctccgg
    17881 caccggacac aactccatga ccaactgggt ggagaagacg aaccggctga cctccaacgg
    17941 cagcgactat ctgcgcgaat ggcaggacgg cggcgcgccg atcaacatga cgaagaccga
    18001 cggaacgaaa taccagcagg tggtgttcgc cgattggttc gtggaggcga aggccgcggg
    18061 ggcttccgcc ccggtgcttt tgtcccactg gcagccgcac gccggttttt cgggaggcaa
    18121 tccgctggcc accttcgtgg acgccgacaa caacacggtg gacgacgatg tcgacctcga
    18181 cggcgtgttg gacatcccgt cgggctacgt caactacgag agcgccgacg cgggcgtcac
    18241 cgtgggcgac acccggggga tagacgccgc ggggttctcc aacagcaagc gcctggacta
    18301 tttcgccgac gccgccgtgg ccgcttccta taagggcagc gagggatacg cccgcaaggc
    18361 cgagaccctc ttcaacgaca agacctgggc cgcctcctcg tcgctggaga tcctcagctc
    18421 caacaagatc tcccagttga acacctacac cgtgcccggt cagtccgcca tcacgctcca
    18481 ggtggaccag tattatttca acgacgacgg cgaccggttg agctttttgc aggcggcgag
    18541 tctgacgtcc gactccattc aaggcatcaa ctacgaatcg gtggccacgt cttccctgta
    18601 tggcgaccgg aaactcgacg tggtgatcag cccgtccatc ttcaagaaag cggggttgtt
    18661 aaacaactaa catgaacatg cgccggattt cccgaaccgt aatctttgcc gcgttctgcg
    18721 gcgtggtggc ggcctccgct tcggcggagt tccgttgggt cccttacctg aacttggaat
    18781 cctatctcgg ccgctccaac ttcgccgaca gcgacgtgaa cggtttcaac gggaacctgg
    18841 tcttcgtccc cggcatgcgt ttcggggaga aactatccgt tctgcccacg gccatgctgg
    18901 gctaccgcaa gtcccgcgac gttcaggaac tggccggcgg cggcttcttg acccaggaac
    18961 agcagacccg cggactctcc gtcaagggca tctacgtgct gacccccggc tggaagggca
    19021 aactctacac ctcctaccgg caggaactgg tcaaggaaac caaagacgag aaatggggcg
    19081 acggtctttt cgattacgac aagaccgcgg tgggcgtgga ggtggagcgg gagggggatt
    19141 tctggaagag cgtgcgcctg ggggcggact attacacgac ggccttcccc aacttccaga
    19201 gcctggccac caaacagtac ggcgcggaga tcaacgccgg cgaggatgtg ttggatttca
    19261 gcgcgatgga tttctccgtc ggagcggacg ccgcggtgac ccggaagtcc atgctctccg
    19321 gctacctcct cggttctcgc cgcgactttt ccgaccagaa gatcgtcaag cgggacggca
    19381 ccttttccgc cgacacgcgc aaggacatgt tcacctacgt gtccttggcc taccgctatc
    19441 agctgccgac cgtggccctg ttccatgtgg gcgtggaaag cctggccggc ctggacgcct
    19501 cctacgccct gctgaactcc gaccagcaca actacgacgc cggccgcacc aagttcaacg
    19561 agaactatta cgactacgcc gaactgggga tcggcccccg cgtcaacctg cggttcaagg
    19621 agaagctctc cgtcggcctg tcctatctgt tcagcacgcg cgactacgcc gaccggcccg
    19681 tgcagcgcgt ggacggcacg tataccgcgg acaccatctc ttcggacgtg accaccttcc
    19741 gcgcctcgtt gggctatcct ctcctgaagg gactttctct ccaagcccag ggggcgtggc
    19801 agaacgccga ctccaacatg gattacgaga cggtctatcg ctacaattac tcgtcctcgc
    19861 actatttcct ggggatctcc taccagctct agaatatacc gggggcggtg ttgacggtgg
    19921 cgtagacccc ggactctgtc tcggagcttc gttcccaccg gaatcgtccg tggttcgagg
    19981 ctacgggcga ttggcgggcc gccggctcgc ccttgaccgc tcaccggcgg atgtggtatg
    20041 cttatcaaca agtggctgaa gttttggcca cttttttgtt tgtaggggga gccccggatg
    20101 ggcatggttg acgaagtcag gacgctggtc tcgccggtcc tcgcccaaga gggcgtggaa
    20161 ctggtggagg tggaatacca gcgtgaaccg aacggctgga ccctgcggtt ttatctggac
    20221 aaggcgggcg gcttttcctt ggccgattgc gagcaatgga acgaccgttt gggggaggtc
    20281 ctcgaccggt ccgaactcat ctcccacgcc tacagtctgg agatctcctc gccgggactg
    20341 aaccggcccc tgcgcaaacg cgaggatttc gaacgttttt cggggatgga cgctttcgtc
    20401 aatctctacg cccagcagaa cggccagaaa cattttcacg gccggctggt gcgcgttgaa
    20461 aacggggaac tggtcttgga agaccgcacc tcgggttttg tgaaaatacc gctttcctcc
    20521 atcgccggcg cgcggctcga cccgcccgtc gcgttgggcg gcaacgctga aaaggacatg
    20581 tcatgacgca aatactgaag aacgaactta tgcccgtgct cgaacagatc gaacgcgaca
    20641 agggcataaa aaaagaggac atcctgcaca tgatcgagca ggcgctggtg tccgcttatc
    20701 ggaaacacgt gggccagcag gtcaacgtgg aggccaagat cgacccggag agcgggcaga
    20761 tcaaggcgag cgtcgtcaag accatcgtgg agacggtcac caatccggtg atcgagatct
    20821 ccgtggccga ggcgaagaag gtgcgcggcg ccggagacgt cggcagcgag ttcaagatcc
    20881 ccgtggatgc cgccgagttc gcgcgcatcg ccgcccagac ggccaaacag gtcctcgtcc
    20941 agaagatccg ggagacggaa cgcgagacgg ttttcagcga atacaaaccc aaggaaggca
    21001 ccctcatcaa cggggccgtt caccgctttt ccaaccggaa tctcatcgtg gacctcggcc
    21061 gctccgaggg gatcctcccc gtgcgcgaac aggtccggcg ggaacgctgg accgtgggcg
    21121 accgcatccg cgccgtcatc ttgaaagtgg aaaaagggcc gcgcgggccg gagatcatcc
    21181 tcagccgcgc ccacgccgat ttcgtgaaac ggcttttcga gcaggaagtg ccggagatct
    21241 acgaaaagac cgtggaggtc gtggaggtgg tgcgcgaggc cgggttccgg tccaaagtgg
    21301 tggtgcgcag caacaacccc aaagtggacc ccatcggcgc ctgcgtgggc gtgaaggggt
    21361 cccgcgtgcg gcccatcatc aacgaacttc agggggagcg catcgacctc gtcgcctaca
    21421 cgcccgacac caccgctttc atcgccgcct ctctttcacc ggtgaagccg ctttccgtca
    21481 ccgtcatcag ccacgaggag aagaaggccg aggtgctggt gagcgacgat caactgtcgc
    21541 tggccatcgg caagaacgga cagaacgtgc gtctcgcctc ccgcctcacc ggatggcaga
    21601 tcgacgtgcg ctccgaatcg cagaagaaag aggccaacgc cgccgacaac gccgcgcaca
    21661 tcagcgccct gtcccagctg gagggggtcg gcccgaaaac cgcggacgtg ctccgcaaag
    21721 gcggctgggg ggacatttcc cgtctggccg gcgccaaacc ggacgatttg accgcgctct
    21781 ccggcatcgg tgaaaaaacc gcggaaaaga tcatcgagtc cgccaaggag tatctgaaga
    21841 aaaaggccga agaggaggcc gcggccccct cgacgccgcc ggccgagccg gaggcggttt
    21901 ctccggagcc ggcggctgaa aaagaagcca aagagacgga agaacccgcg gccgaggccg
    21961 cccccgccgg ggaatcggcc gccccagcgg aaaaacccgc ggcggaggga aaaaaatcca
    22021 aacgcaaaca ggaacagggg acggagcaga aagatgactg acgagaagaa gacgaaaaaa
    22081 gctccggcgg cgaaaaaggc cgcttcgccc aaagcttcca aagaggccgt cgccttggaa
    22141 gacgggaaga aaccgaccaa acccaagacc tccgcgaaag ccgtcaagcc caaaaaggcg
    22201 gtggacactt cggcgccggc gacggctgaa ccgaaggccg ccaaaccgaa gaagaaagcc
    22261 gttgcggcgg aagccgcggc cgcccccgcc gaggtcccgg cgaagaaagt tccggccaaa
    22321 aaagtaaaag cgaaggcgcc cgcggtcgaa acaaaagagc ccgcttctcc tccggcggcc
    22381 aaaatttcca gcgcccccgc cgtgccgccc aaaccggcgc cggcggcgcc tccgaaaccg
    22441 gcggctgtcg ttccgcccgc cccacccgcc cccccgcgtc ccaagatcaa ggtcaacgag
    22501 atggtcacgg tgaaggaatt ggccgacaag atgaacgtga aggtgccgga cgtcctgaaa
    22561 aaattcatgt ccctggggaa gatcgtccac atcaaccaac gcgtggacgt ggcgttggcc
    22621 ggcaagatcg ccgattcgtt ccagttcgat ctggaagtcg tcccgacgta cgccgacgag
    22681 gtcctcgatt cgcccgacga cccgtccctg ctcaggtccc ggccgccggt ggtgaccgtg
    22741 atgggccacg tggaccacgg caagacatcc cttttggacg ccatccgaaa aacccgtgtg
    22801 gcggagaaag aggcgggcgg catcacgcaa cacatcgggg cctatcaggt gaagaccgaa
    22861 aagggctata tcaccttttt ggacaccccc ggccacgaag cgttcaccgc catgcgcgcg
    22921 cggggggccc aggccacgga cctggtgatt ttggtggtcg ccgccgacga cggcgtcatg
    22981 ccgcagacgg tcgaggccat cgaccatgcc caggccgccg gcgtccccat cgtggtggcc
    23041 atcaacaaat gcgatttgcc caccgccaac ccgcagcgca tcaagcagga actgacgaaa
    23101 cacaaactca tcgccgagga ctggggcggc aagaccgtga tggtggaggt ctccgcccgg
    23161 cagaacaaga acctcgacac ccttctggag atgatcctct tggagtcgga actcctcgaa
    23221 ctgaaggcca atcccaaccg tccggcccag ggcgtggtgg tggaggccaa actcgatcct
    23281 cgccggggcg cggtcgccac ggttctggtg caaaaaggca ccctgcgcgt gggcgacgcg
    23341 ttcgtgtgtg gcctggcggc gggcaaggtg cgcgcgcttc tggattacgc cggccagcgg
    23401 gggaaagagg ccccgccctc ctgccccgtc gagatcctcg gtttttcgac caccccccgg
    23461 gccggggaca agttcatggt ggtggcttcg gaacgcgagg cgcgggagat cgcggagagg
    23521 cgtcaggcca tcgcttccga cgccgcccat tctcgacggc aacacctcac cctggaaggg
    23581 ctccacgacg aggtggcggc cggcaagatc aagtttcttc ccatcgtcct caaagccgac
    23641 gtgcagggct ctctggaacc ggtgcgggat tccctggaaa aactggccac ctcggagatt
    23701 tcgctgaaga tcatccattc cggcatcggc ggcatcaacg cctccgacgt gatcctggcg
    23761 gccgcctccg acgcggtcat cttcggattc aacgtccggc ccgaccccag ttccgaggaa
    23821 ctggcgcggc gggaaggggt ggagatcaag acctaccgca tcatttatga gatggtcaac
    23881 gtggttaagg cggcgctgga aggcctcctg tcgccggcgg aacaggaggt cacctcggga
    23941 tgggcggagg tccgccaggt gttccgcgtg cccaaggcgg gcacggtggc cggctgttac
    24001 gtgaccgacg gccgcatcgt ccgttcggcc aaggtccggc tggtccgtga cggcgccatc
    24061 gtcttcgacg gcgccatcgc caacctcaaa cgtttcaagg aagacgtgcg cgaggtggaa
    24121 aaaggttttg agtgcggtct gtcgctggag gggtttcagg acgtcaaacc ccaggaccgc
    24181 atcgagtttt acatcatcga aaagaaggcg cggaagctct aaacagagag ccgggatcgg
    24241 aaacccggcg ccggcaaaaa atccgccgga attttcagtt ttgtccggcc tctgaaaccc
    24301 ggccaccgcc gcccttcccg ggagttgtct atgtggtctc gccgtatcga acgtatcaac
    24361 gaactcctcc tccaagagat atcgcgcgtc gtcgtcgagc gccaggaccc ggacatcggt
    24421 tttgtgacgt tcaccggcgt ggaggtcacc gaggacctca tgcaggccaa ggtgttctac
    24481 tccgtcctcg gcagcgagga cgaccgtctc cgcaccgccg gggcgctgga acacatgcgg
    24541 cacgaaattc atcaggacat gcgccgcctg gaaagcctta aacgcatccc caccctcgtt
    24601 ttcatccacg atcagacgcc ccagaaggcc gcccgggttt ttgaactcat cgagcagatc
    24661 catcaggaat cccagccggt gcccccggag cccgagaaga agcctgtcgt ccggccggcc
    24721 tctcgcggac gaaagcccgc gtctccctcc gccctcaaaa aggaaaagaa aagtgcccgc
    24781 ccgcctcagc gaaagaaacc gcgataagat catcacccgc atcctccgga ccgtgcggga
    24841 aaaccgtacg ttcttcctgg ccgggcacga aaagcccgac ggcgacacca tcggttccga
    24901 gctggccctc gccagcctcc tgcgccgctg gaaaaaacag gccgacatct acaattccga
    24961 acccgtgccg aaaaatctgc ggttcctgcc cggcgcggag gccattcgtt cggcgcgccg
    25021 ggtggacaag gcctacgacg tggccatcat cctggaatgt tccggaccgg aacggatggg
    25081 gaacatcatt gacctgaagt cccaggccaa gacggtcatc aacatcgacc accacgccca
    25141 ccacgccacc ttcggccaca tcaacctgat cgacgcgcag gcctcttcca attcagaact
    25201 cctgtactat ttcttccagc acgccgaatt gaaactttcg cgggacgagg cgatgtgcct
    25261 gtatgtgggg ctggtgaccg acacgggccg cttccagcag gtgaacacca acgcccgcag
    25321 tcacgaggtg gccgccggac tccatcgtgt cggaatcgac gtctccgaca tcacccgccg
    25381 gctctacaac acccgcaccg aatcttccct gaaactcctc ggccgcgcca tggggtccct
    25441 gcggctggag ttccagggcc gcgtggccgt gatctcgctg acccagcggg atttcatcga
    25501 gacctcctcc ggcccggaag agaccgagga atttatcaac tacggcctga tggtgccttc
    25561 cgtgcaggtg gccctgttcg tccgagagac cgagatcgcc ggcaagatga aggtgagttt
    25621 ccgcggcaag gggctggtgg acgttctccg tgtggccgtg gcctacggcg gcggcggtca
    25681 caagaacgcg gccggctgca ccgtcgacgg caatctggaa aacaccgtcc agtccgtcct
    25741 ctcccggctg caaaaaatcc ttcccgcctg acgttcctcc ttttgtccga ccatccgcat
    25801 cccttttttt ccggagtcct cctgttggac aagccctccg gccccacctc gcacgacgcc
    25861 gtcctttggg cgcgccgggc gctccacacc aagaagatag gccattgcgg cgccctggat
    25921 cccctggccc agggactcct gcttctggcc atgggggagg cgctggaatg gcaggagcgc
    25981 cttatgcgcc agcgcaaggt ctatcggggc tcttttcggc tcggcgtggt caccgacacg
    26041 gacgacatca cggggcagat ccttcggcag gaaacggacg cgcggagggt cgcggcggtg
    26101 tccgatgagg cgctggccgg gctctttaaa cgattcctcg gccccatccg tcagcgcgtg
    26161 cctcgatatt cttccgtgaa ggtgaacggc cgccgcctcc acgagtgggc ccgccggggc
    26221 gtggagatgc cgcttccgga aaagaccgtg gagatcagcc gcttcgacct cctctcctat
    26281 gccccgcccg acgcgcattt cgtggtggag tgttccaagg ggacctatgt ccgctctctg
    26341 gcgcgggacg tgggggaaca actcggtttg ggcgccaccc tggcctccct gtgccgtgaa
    26401 agcatcggcc ggttctcccg ggcggacgct ttcgcgtgga acggggattt ctccatggag
    26461 ccgacggact gggaacgcgc cttcatcccc atggacgaac tgccggcgct ttttcccgcc
    26521 ggggaaccgg cggccgtccg atgagcgctc cccgacgagg gacggtcctc accatcggca
    26581 cgttcgacgg ggtccacgtc ggccatcggg tcatccttcg gaaggcggtg tcgctggccc
    26641 ggaaggcggg gctgtccagc ctggcgctca cgttcgcccg tccgccccgc ctgtatttct
    26701 cgcctcaaaa aggtccgctg ctcatcacgt tgccggaaga aaaggccgag ctcctgcgtt
    26761 cgatcggcgt ggaccgcgtg gagtttctgg cgttcgggaa aaaactggcc gccctctcgc
    26821 cggaggactt tttcaaggaa catctcctgc gccgctacgg ggcccgacag gtggtggtgg
    26881 ggttcaactt cggctttggc cgccgccgcg ccggccacct gggcctgctg aaacagttgg
    26941 gcgcttcgca cggcgtgccc gtgcacatcg tgccgcaggt gtctctggga ggccgacgcg
    27001 tctcttcggg aaagatccga cgggacctct ggcgcggcga actggccgcc gccaaccgta
    27061 aactgggata cccgtatttc ctttccggcc aggtggtgcg gggggaccgc ctgggccggc
    27121 ggctgggttt tcccacggcc aacgtgcggg tgccggacga aaaactggtg ccgccgggag
    27181 ttttcgcggt gcgggtggtc ctgccgaacg gcacggagaa acccggcatg tgcaacgtgg
    27241 ggttccgtcc gaccctgggc cggcgggctc cgcgcctgtc catcgaggcg cacgtgttgg
    27301 atttcagggg gaacttgcgg ggtaaaaaaa tcaaagtggt tttcgtgaag aagatccgtt
    27361 cggaaaaggc gttcccgacc ctggaaaaat tggccgctcg tctccggatc gacgccgccg
    27421 tcgcccggcg gatcctgcgt cgatccgcat gacgcgccgc ctggccgtct gtttgctggc
    27481 cggtttggcg gcgtcttcgg tttttgccgc gcaggaactc ccgcatttca tcgcctcccg
    27541 ccatttttcc gtccaatacg aatcccctta tccgcccgcc ggcgttttga acacgttgga
    27601 gggactgcac gccaaactgc ttttggacct gttgccgttc gcgccctggg ccaaggacgg
    27661 aaaaatacag gtcgttctct attcgagcgc cgagcgttac cgtgcggtgg aggtcatgcc
    27721 cgcctgggcc ggcgggcatg tcgacgtgaa ggcccgccgg atttttcttt tcgagggcga
    27781 cgagttccgc cggaccatgg cccacgagat ggctcacctg ttcgtgcagg actacttcgc
    27841 cgcccggggg agcgtgccgc cggattggct gaacgagggc gtggccatgc gcatggaagg
    27901 ggactacggc ggcgcggtct cttcgggcgg ccgggaactg aaagaagcct ccccgtcgtt
    27961 ggaagctttc ttcaatctca actatcgtca tgccgcgcag aggcccgagg aggtggcgct
    28021 gtggtatgga caggcgggaa gtctggcggg ctatttgatg cggcaattcc ctcggggaca
    28081 gttcgtcgcg ttctgcgacc ggctgcgcaa cggcgcctcg gtgacgctgg aacc
//
LOCUS       DAOTFD010000012        27617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1129_length_27617_cov_4.495828, whole genome shotgun sequence.
ACCESSION   DAOTFD010000012 DAOTFD010000000
VERSION     DAOTFD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 27617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27617
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_27617_cov_4.495828"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="P5079_01565"
     CDS             complement(<1..692)
                     /locus_tag="P5079_01565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28706.1"
                     /translation="MTQQEAPSQAQTFFPPPDRGWDRALVFLLLGLFLVLAVWDLESP
                     ALHRDEALIGRLAASIDFSQALETWSFRTLPLQADQHHGTWMAYAVYPFLKLFGGQPV
                     AVRVAGVVYGFLGLAAMFCLTHRLFGRAPALFFLLLLSLHPSFLCTTRLGMIDGNLMY
                     LVSFAALFFLWRWWTDGRFGHFLWTCFFLGMGVGILSWFIYALGALGVAMSLKARGVA
                     ERVGRRWRWYLLG"
     gene            complement(741..2438)
                     /locus_tag="P5079_01570"
     CDS             complement(741..2438)
                     /locus_tag="P5079_01570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28707.1"
                     /translation="MKSLAAPAFHAITAAVGGAFRYLNLFALRTSRGHKAALFGALVL
                     FLLFALWDLKSPGIGRDEASMGYAAAGIDFLHALKNLSFERLPLQFDGHHGTWIAYSV
                     YPFLKIISDPVVAMRLAMVLYSVLTLLCLYRLVFDLYGEGAALLSLSFLAFHPAFLLI
                     TRMGVRDGNLLLLFSVLAVSFLIRWWKGGRTKDFFLMCFTLGLGLGTLSWFIYWMAGL
                     SATLLCFGRGFFRRLMAAWKSHVLGGGVCFLLGVGPLFLAMLFSDPPFFQPIEYLIVL
                     WSGHSQGVGLSSLLVFQPQRSFAIFRAVLEGGYTFLSYYWDLSVGNLRGYYTLFLVSA
                     GWLVARSVFRRQRSFETGLPLALVCLVFTTLLFASFEPLGVHPAHLLPILPWAVLICA
                     IGVHDVCKAIPNEPVKRLVMGVVPGVVLVSVLFFDVEGYVYLHKTGGGTADNSGAVYD
                     LAVWLKKRNPQTIMVTIRAVDRIKFLVSPAVQLKDMGIYVAATEEGTFQNLTKISKKI
                     DVYVAPIGNHSGSPATDMFYLFNKWRKQQTSPVIRLKTVFYDRHGRPAYRVYEPQRS"
     gene            complement(2649..3938)
                     /locus_tag="P5079_01575"
     CDS             complement(2649..3938)
                     /locus_tag="P5079_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRY28708.1"
                     /translation="MTKFVADLHIHSPYSRACSKDLRPESLYRWCQLKGVAVLGSGDF
                     THPAWFAELRDKLRPAEEGLFELKPELAEKVNGEIPPTCRAPVRFLLEVEISCIYKKA
                     GRVRKVHHLIYAPSFEVAGRLRESLEKIGNLRSDGRPILGLDSKDLLKRVRDVSDRAY
                     LIPAHAWTPHFAVFGSESGFDSLEECFDELAPEIFALETGLSSDPPMNRRVSGLDRLS
                     LISNSDAHSPEKIAREANFFDAPFSYEGIFDALRRRPGGRLTATFEFFPEEGKYHLDG
                     HRKCQTRLTPKETQEKGGLCPVCAKPVIVGVLSRVEKLADREAGKKPSSASAYESLVP
                     LKELLSETLDVGPGSRKVDAAYHRLLSLFGNELFVLRELPPGRLEAEGFSLLALVLTR
                     MREGKADIRPGYDGEYGVVSFLRPEDRFSGTEAAPVS"
     regulatory      4073..4243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4314..5669
                     /locus_tag="P5079_01580"
     CDS             4314..5669
                     /locus_tag="P5079_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRY28709.1"
                     /translation="MWQRIKGWVLGKEKNPQDPEVFHKVSLIAFFAWVGLGADGLSSS
                     AYGPEEAYRALGAHTHLAILLVAMTAITIFVISIAYSNLIQHFPGGGGGYLVATKLLG
                     PKVGVISGCALLVDYVLTISVSIASGCDALWSFFPAGAAPFKLTGAIILLFLLLALNL
                     RGIKESVTVLAPIFLLFIVTHVFMIVYAVTSHFGGLPRIFHGAAADFHSSVSSMGFLP
                     VALILLRAYSMGGGTYTGIEAVSNGVAILREPRVQTGKKTMFLMATSLAFTAGGILFA
                     YLLTDARPVFGKTMNAVLLGNLFGSWEWHGLNIGYDFVVIALVSEAALLLVAAQAGFL
                     DGPRVLANMASDSWIPHRFSQLSDRLVTQNGVLLMGGAALATLYYTNGNITTLVVMYS
                     INVFITFSLTELGMARHWIVRRRTEPKWKSQLTVHGTGLVMCLSIPPLLFTKNSRRAG
                     G"
     gene            5774..6298
                     /locus_tag="P5079_01585"
     CDS             5774..6298
                     /locus_tag="P5079_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28710.1"
                     /translation="MPEKASAKIPEKSFRLPAALLMVNGYNGFGIHSFLAIHRFFPGH
                     FKNFVFLSVGAVDSSRFKGAEELQKLKESVSADLEKYVKLANGMSLYAESHMLLETDV
                     VEGLETLCHREGAHWPKKSYFMGQLVFEADNFWNRFLHNQTSFTLQRRLIFRGYEVVV
                     LPIRVRLQQTRLPG"
     gene            6380..7270
                     /locus_tag="P5079_01590"
     CDS             6380..7270
                     /locus_tag="P5079_01590"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY28711.1"
                     /translation="MNALPSVGFIGLGIMGLPMARHLLAGGYPVTVFNRTASKAKTLE
                     SVGAKVAAHPKETASRCRVLITMLTDPAAIRQVMDGPDGFLAADNPDVVWIQMSTLDI
                     ASTMDFAATAKKKGWGFLDCPVTGSKKQVEAAELILLAGGPAGLLEAHRPLLMKMAKA
                     IVHAGDVGAGSALKLCMNLIVAQMTTGLAEAAALADTCGIDPARIFDVLKESPALNCA
                     YYQIKKEPVLKKDFSPAFSLANMLKDVRFMTKEAGGRGLDLPVTQAVRALMESALQEG
                     RGDKDLMSIYLTLRRNKKSG"
     gene            complement(7456..7995)
                     /locus_tag="P5079_01595"
     CDS             complement(7456..7995)
                     /locus_tag="P5079_01595"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRY28712.1"
                     /translation="MFKRLAVGEFYHLLGFGPCVLITSGDEKRGNVAPIAWTTPVNDD
                     PALAGIVVAETHYTTELILRKKQFVINVPPLELLKPLKFCGSVSGRKGDKFAKTGLRL
                     ERGVKVRTPHLKDAIAFIECALLWKRNFGGVYLFVGKVLHAAAEKSCYKNGVLTARAR
                     TFHHLAGGSFALTGRRVSA"
     gene            8183..9478
                     /locus_tag="P5079_01600"
     CDS             8183..9478
                     /locus_tag="P5079_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28713.1"
                     /translation="MIKIFPPQRGAALLLLWTVAVGPFPLTAAEDPSPSGSVPTLRDC
                     YRFALKKSEAVALSAEEIRLAEAQYLVARSGVLPQVGFNLIELFQDEGNTSSDSIRRT
                     QTTEKFYAKQLLFSGFREMAALKGVKAQIAGQTFALDRAKQLLFADVALAFFNVADFD
                     RRLLTLGSMKSAAADRLSELQGREKIGRSRRSEVLSAQTQIATLDARIEEARKLRAAA
                     LELLNFLTGQPIRGIADDVADPAPPPPLEDFLAGASSRPDVRAADEERRAAESFLSAA
                     RRASWPTLNLQGGYYVRRDGTYEPVDWDAQLTADLPLFTGGSLKGGRRAAGARARTAA
                     LAFSRTTRQAESEVRSAHQSLSGSLSQIQKLATALELAEKNYRVQTEEYRLGLVNNLE
                     VLSSLSSLLETRLAYDQALLNAKLDAVRLAVAAGKEPLP"
     gene            9475..12645
                     /locus_tag="P5079_01605"
     CDS             9475..12645
                     /locus_tag="P5079_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY28714.1"
                     /translation="MKLSDLSIKNPVFAWMLMFALMLFGAIGFSRMGVSQMPDVDFPV
                     LNVRVTWEGAAPEVVEANVVDVLEDAIMSVEGIREVSSSSRQGNASLTIEFELNRDID
                     AALQEVQTKVAQAQRRLPRDIDPPVITKSNPEDQPIVWISLSGDRPVKDLMLYADEHL
                     REKFQTIPGVGEVFMGGFTARNLRVWVNADKLSAKEMTVDDILSAVANQHAEVPAGIM
                     DTGPQELNVRSMGEAASVQEFRKLILTQRGGSPVYVPIKLGDVAEVEDGLDDMRRISR
                     AMGKTAVGLGIRKQRGSNAVAVADRVIARMNELRPQLPQGLELEVNFDSTKFIKDAVH
                     ELNFTLVLSALLTSLVCWAFLGSWSSTVNVLLAIPTSVLGTFIVLYFLGFTLNIFTLL
                     GLSLAIGVVVDDAIMMLENIVRHRERGQERVSAAVYGAREISFAAMAATAAVVAIFAP
                     VAFMKGIIGKFFFQFGVTISVAVLLSLLEALTLTPMRCSQFLNVRPRTTWFGHGVEAA
                     FLWLARRYREVLEICLRWRWTVILLSVLFFSASLYVYKGIPKEFVPAQDQSLFLVRLQ
                     TPVGSSLEFTDDRFKKAEAYLAARPEVRRYFGSVGGFGGGEVNSGIIFVTMREPKDRP
                     RNPETGEPVTQQELMTLCRKALNAIPDVRAVIQDPSTRGFTAQRGFPVEFTIRGPDWA
                     VLADSVKKVMDRMEASGLMLDVDTDYKVGQPELRIYPNRAAAAARGVDVAAIGRTINA
                     LIGGVRAGQFTESGKRYDIRVRLVPSERSRPEDINRLYVRNNHGERIRLSELVRLEQT
                     PTLQNITRRQRERAIGVFANVAPGKSQEKALAEVEKIGKEVLPDGYHVVMGGGAKTFQ
                     ESFQSLFFALWLGVIVAYMVLGSQFNSFLHPFTVLLALPFSATGALLALRLAGLSFNI
                     YSMIGFILLMGIVKKNSILLVDFTNQVRARGKDVHGALLTACPIRLRPILMTSLSTIA
                     AAVPPALALGPGAETRVPMAVAVIGGVCVSTLLTLFVVPCAYSLLIPIERRHPHNILE
                     TLEPLLEEHDPPPQAPPTGRRPPDGR"
     gene            12840..14693
                     /gene="typA"
                     /locus_tag="P5079_01610"
     CDS             12840..14693
                     /gene="typA"
                     /locus_tag="P5079_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRY28715.1"
                     /translation="MTTTAHHRRKNIRNIAIIAHVDHGKTTLVDAMLRQTGEFKVKPA
                     EAQECVLDSNPQERERGITILSKCTSVPYKDVLINIVDTPGHADFGSEVERILCMVDG
                     VLLLVDALDGPMPQTRFVLRKSLELGLRPIVVINKVDRPFADPLKALDGTFNLFLNLG
                     ATDAQLDFPTVYASGKEGWASLDHEKPGNTLTPLFDVILKHVPGPVANADKPLQMQVT
                     MLDYNSYVGRIGVGRLMNGRVTQGQTVALLKTDGRTFNYRVTKMYGYFGLEKREIAKA
                     EAGDIVALAGLEEVHVGDTVASVESPEALPPMEIDEPTLSMEFMVNESPFAGRDGKFL
                     TSRHLRARLEQEKQTNVGLRIEELDRASAKQGVFKVSGRGELHLSVLIETMRREGFEL
                     AVSRPEVIYKNRDGAVLEPAEYLVLDIDRQYQGAAMEQLGRRGAEIKHMTMEGENTLR
                     LECVITARALIGFKSELMTQTKGTGLMHHSFHGYVPKTAEPGHRAAGVLVAKEKGSTT
                     AYALESLQERAVMFVGPGVEVYEGMIVGMNSRENDMVVNPCKKKALTNMRAAGSDDTI
                     QLTPPKIFTLEQAIEFIETDELVEITPDNIRLRKKELNFSARRKNERDESE"
     gene            14826..16883
                     /locus_tag="P5079_01615"
     CDS             14826..16883
                     /locus_tag="P5079_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRY28716.1"
                     /translation="MAANRLAAEISPYLIHHADNPVDWYPWGPEAFARAKREDKPVFL
                     SVGYSACHWCHVMERESFQNGSIAAVLNEHFVCIKVDREERPDVDEFYMASVQLFAGR
                     GGWPMTVFLTPDGEPFFGGTYFPPENRAGQAGLKELLEGIARAYRDERPRIRTGAAAI
                     VAQLKKNFRPAAPGRPLSSHGIERAGRELLLQKDPLHGGFGDAPKFPRSSDLQFLMRY
                     WRKSGDRDALSHVQLTLDKMAAGGIYDHLAGGFHRYSVHSDWSAPHFEKMLYDNALLP
                     RAYLDAYQAAPHPRHARVVRETLEYVLREMSDPAGGFHSSQDADSEGREGSYYLWTLR
                     EIHDVLGPEDGPLAGDYFNVRAGGNFEAGQNVLRITETDNAFCARHRIDPLLWPERRR
                     SFCRKLLEIREKRVKPDKDDKIITAWNGLMIGTLARAGRILQERSYETAATRAAYFLL
                     NNMFHEGRLFRAWRGGQAKWKAPLEDYAFLAAGLLDLYEATGQTRWAKEALVLNNVML
                     KLFWDKTQGGFFFVGEDENGAPDRLKTAGDGVLPSPNAVAAMNLLRLHELTLDPELRE
                     MAHKTLRLFSGDSGRAGIGDPSLLAALDFHLEPPLSIVLAGEPREADRHPFLEPLEAA
                     YFPNKALSFAHAGEEAEEKLFPLLKDKRPVDGKPTAYVCQNAACSAPITTVQALLARL
                     QEK"
     gene            16885..17988
                     /locus_tag="P5079_01620"
     CDS             16885..17988
                     /locus_tag="P5079_01620"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRY28717.1"
                     /translation="MRTSARLKTVLLSFAVTPTLLSSFFFFAQAQEPNHPPRRDSMEK
                     YTKPSPETLKKRLTPLQYDVTQCSLTEPPFQNAYWDFKEAGIYVDAVSGEPLFSSLDK
                     FDSGTGWPSFTKPLADENITEQADLSHGMVRREVRSKHGDSHLGHLFPDGPGPTGRRY
                     CINSASLRFIPTADLEKTGYGEYRSLFAKSGKPEKPAPTSRLETATLAGGCFWGVEDI
                     FAKIPGVASTEVGYAGGDRDRPSYESVSAGNTGHAETVQVVFDPTKITYAEILSYFFR
                     LHDPTTPDRQGPDIGSQYRSAIFYHTPEQKRTAEEVKKMVADSGRWKDPVATQIAPFK
                     AFHRAEEYHQKYLSKNPDGYHCPTHFLRKPYKK"
     gene            complement(18041..19570)
                     /locus_tag="P5079_01625"
     CDS             complement(18041..19570)
                     /locus_tag="P5079_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRY28718.1"
                     /translation="MKHLRAKKPLSLLMEEMKGENRLRRVLGPVQLTSLGIGCIIGTG
                     IFVLTGIAAHDKAGPALIVSFLVAGLACIFSALCYAEFASLAPVAGSAYTYAYATLGE
                     LFAWIIGWDLVLEYTVASATVAHGWSHYFQNFIGIFRLKIPEALSRAPFDFSPELGRL
                     VSTGTVMDVPAIVITAAITIILVIGIKESASFNTAMVVVKVAVVLFVIVVGAFYVNPD
                     NWSPFAPFGFSGISFFGKTLIGPAGLGGEPLGMMAGAGMIFFAYIGFDSVSTHAEEAR
                     NPQRDVPIGIIASLVICTILYIAVSAILTGMVPYDQINIDAPVAEAFRAVGLPWAQFI
                     ISLGAMAGITSVLLVLMLSQPRVFLAMARDGMVPSGVFGAVHPRYRTPWKSTILTGGV
                     VALLAAFIPLRVLAELVNIGTLLAFLIVCAAVLIMRKTDPDAPRPFRCPWVPWVPFAG
                     IATCLLLMFSLPTENWLRLLVWLLIGFVIYVSYGRHRSILAQHRARAAALPPQKTPPL
                     K"
     gene            complement(19661..20194)
                     /locus_tag="P5079_01630"
     CDS             complement(19661..20194)
                     /locus_tag="P5079_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRY28719.1"
                     /translation="MAKIVYYVASSLDGFIARPNGDISWLRPFEGTGEDYGYREFYDS
                     VERLVLGARTYEQIPSLGAWPYSGKPVHVVTSRPLPRFDPRVVFQSGDFQKWVPPLRR
                     VSEKNIWLVGGGALAGAFLQEDFLDELIVSVVPVLLGEGIRLFGSLKTAVSLRLMRTQ
                     EFENGVMQMRYRVRGKD"
     gene            20412..22328
                     /locus_tag="P5079_01635"
     CDS             20412..22328
                     /locus_tag="P5079_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY28720.1"
                     /translation="MKNSFFFGLFSWKLPLPGRAAFRILKLAALFYFFPLVSFAAMLS
                     PQVSQFIHSNDPSKKPIIVIYKRDNSFRKADPRVSASPAAVLRAEADGTRPAPSALPV
                     SPEPRGPVDLWLLNGYAVEATPEETRRLAADPRVESLHENFQLRLPDILPQNHRTENV
                     EGNWGLEKSGAKMAREMFGVTGAGVRIGHLDTGLDASHPALQGKLAAWAEFDADGRPV
                     PGSQPHAAGFHGTHTAGILVGNTAGGEPLGVAPDAKLLSALVLNSDGGTFLQVIAGMQ
                     WALDPDGNLSTDDGARLLNMSLGANGHYAVFDAVVDRLLEVGVLPVFAIGNGGPGQTT
                     SPGNVRGVLAVGATTEEDTVAYFSGGEEKMGAGQPLLKPEISAPGSGIRSTMPGGTFA
                     SYSGTSMATPHVAGAAALLWEANPSMTASQVKDLLMQTSVDIERPGPDERAGSGRLDV
                     LAALSRLRGIPLLRVATNGPHGPVPAVVRIHNTLGSNLRVVHVSPKTGVFCTALPEGD
                     YTVEAAVGLDSPARLPVRMEPGKVKDLKFVLNDAPKTLGVLLVYPNPFKPYERHEAVT
                     FAGLPVDSRVQIYTLSGALVRESGSLPVNEYVWDGRNGDGEPVASGVYYYVASHYDIR
                     SGWTRKKGQLVVIR"
     gene            22355..23347
                     /locus_tag="P5079_01640"
     CDS             22355..23347
                     /locus_tag="P5079_01640"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY28721.1"
                     /translation="MRLLLVAEMYPTGTVSAGAGAGTTAADFLLTPPDSRSAALGNSG
                     VADAESPFSLYNNPALLSDAKSPLTLAGGVAEWPLGLQAQHYAVSSVWGTPADGPWGL
                     GIFLSQWQTAPIQATDIYGNANGRVRYTARNVGLGLAHALSPALHLGGAVKTISQRFE
                     ETGRAGGNTGTGHSALAWDFGVAYDTPLKYVRVAAALQNTGNDVAFRQDQEKLPRALR
                     LGMQGAFFNRQFQWAAEGRKLSGGALAVKGGMGFRPFPNMILRMGFDSGLRDIFLGVT
                     TGVGFRLRNVDIDYAFVPLGNLGTTQRMTLAWRIGKTAPRTRNAERPPSLWSAN"
     gene            23401..23763
                     /locus_tag="P5079_01645"
     CDS             23401..23763
                     /locus_tag="P5079_01645"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRY28722.1"
                     /translation="MEMSEFEDIIEATVKKMPARFRKILKNEGIRLIARGKTPGVLQD
                     RYRGDWVFGVFLGVPYNQRSVFNVEQEPTRIELYRESFEKAFRRRREMEEQIARTVIH
                     EIGHYFGFSEKDLRRHRL"
     gene            23897..25195
                     /locus_tag="P5079_01650"
     CDS             23897..25195
                     /locus_tag="P5079_01650"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRY28723.1"
                     /translation="MKTKMKSSTPAPSSHSAPVNAWDMALEQLDTVAKHIRLDRGIHE
                     FLRYPKTILSVSVPVRMDNGTVQVFEGHRVQHNVTRGPAKGGIRFHPDLTLDEVKALA
                     FWMTLKCAVMGLPYGGAKGGVRVDPKKLTLGELERLTRRFASEIAIIIGPEKDIPAPD
                     VATNPQIMAWIMDTYSMGIGYSAPGVVTGKPLEIGGSLGRNEATGRGVVVIIEEAFRR
                     LKWPLRGATAAVQGFGNVGSVCAKLLAGMGVKVIAVSDVDGGVYSERGLDIEKMLAAK
                     AKGAGVKDFHAPGTTRITNEELLECRCDVLIPAALENQITKDNAPRLKARMVAEAANG
                     PTTPEADRILLKKKIPILPDILTNAGGVTVSYFEWVQDIQSFFWNEDDVNKRLKEIMM
                     GAFNNVFQEAQTGNIDLRLAAQVVAVRRLAEALKIRGVYP"
     gene            25392..26318
                     /locus_tag="P5079_01655"
     CDS             25392..26318
                     /locus_tag="P5079_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRY28724.1"
                     /translation="MSNSLYVVVGATGHIGKEIVLGLLAKGKKVRAWARTASKLEELA
                     ARGAETLEIDLQDGAAVKKGFDGATAAFVMIPPNYTAPNFRDFQNRIGQALADGIRAS
                     GIKHVVNLSSIGAHLPDKTGPIAGLYDQEQRLNRLSDVHIVHLRPAFFMENLLGNIPA
                     LLQMNVLGSAMKKDLAFPHIATRDIAAVAVDLLDKLEFTGKQVRELLGPRDVSMGEVA
                     RVFGAAIGKPDLPYVQFSYSDVEKAFLHMGMSPDAVRTFIEMARGFNEGLVVATQPRS
                     ALTNTPTDIEQFASVFAAAYKAERESRAVAAR"
     gene            complement(26349..26906)
                     /locus_tag="P5079_01660"
     CDS             complement(26349..26906)
                     /locus_tag="P5079_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28725.1"
                     /translation="MTNLAGEKSSDRPAPYGQSVAYAGLSKTHPRLTFNDFSLQYVGL
                     REVPGPNNAPWKMTVYRFHVQAREAQQEILWSSGSGLIAPSSFVVGHVRYLLELKHSE
                     KQGKLPDGFLVVSPAGEAISKEEAVEIARKDAGQAVGSLARYEILAHPRPNGWHVDFV
                     HKKKKTYDGPGYVIGYDGRIVSKKP"
     gene            complement(27047..>27617)
                     /locus_tag="P5079_01665"
     CDS             complement(27047..>27617)
                     /locus_tag="P5079_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY28726.1"
                     /translation="LLTGIAYRVKGVEHDLKRGKPPAAKETARILECINNGISQTKSL
                     SHMLYPAELEEEGLVPTLKNLAGQTAVIYNVACSLKVNGKVSRKNDIVSMHLYRIVQE
                     SIHNAIRHGRASRIKILLTSRSSEVEVRVADNGKGFDPQKTEGGLGLKILQYRAKEIG
                     GNLAIRKNVPQGTVVVCRFFSHGGKRGTK"
BASE COUNT         5647 a         9113 c         7891 g         4966 t
ORIGIN      
        1 ccgaggaggt accaccgcca ccggcggccc acccgttccg cgaccccgcg cgccttcagc
       61 gacatcgcca cgcccaacgc ccccaacgca tatataaacc agctcaggat ccccaccccc
      121 atgcccagga aaaaacacgt ccacaggaag tgcccgaacc tcccgtccgt ccaccaacgc
      181 cacaaaaaaa acagggccgc gaaggaaaca agatacatca aattaccgtc gatcattccg
      241 aggcgcgtcg tgcacaggaa ggacggatgg agagacagca ggagcagaaa gaaaagcgcc
      301 ggcgcgcggc cgaacaaacg gtgcgtcaaa cagaacatgg ccgccaaccc gaggaaacca
      361 tagaccaccc ccgccacccg gaccgcgacg ggctggccgc cgaacaactt caaaaaagga
      421 tacacggcat acgccatcca cgtgccgtga tgttggtcgg cctgcagcgg cagggttcga
      481 aaagaccaag tttcgagggc ctgcgagaaa tcgatggatg ccgccaaacg tccgatcaac
      541 gcctcatccc ggtggagcgc ggggctctcc aaatcccata cggccagaac gaggaacagc
      601 cccagcaaca agaacacaag ggcgcgatcc cacccccggt cgggcggcgg gaaaaatgtt
      661 tgagcctgcg acggggcctc ttgctgcgtc acgagcgctc ctcaccgcaa cggcgcgctt
      721 tttctctttt ggcggatctt ttacgatcgc tgaggttcat aaacacgata ggccggccga
      781 ccatgccgat catagaaaac ggtcttgagg cggatgacgg gggaagtttg ctgtttcctc
      841 cacttgttga acaaataaaa catatcggtc gccggactgc cgctgtggtt cccgatgggc
      901 gccacataga cgtcgatttt ttttgaaatt tttgtcagat tttggaaagt accctcctcc
      961 gtggcggcga cataaatccc catgtctttg agctgcaccg ccggcgagac aaggaacttt
     1021 atcctgtcca ccgcccgaat cgtcaccatg atcgtttggg gatttctctt tttcagccaa
     1081 acggccaaat catagaccgc cccggaattg tcggcggttc cgccccccgt cttatgcagg
     1141 tatacgtatc cttcgacatc aaaaaagaga acggagacga gcacgacgcc cggaaccacc
     1201 cccatcacca accgtttcac gggctcattc gggatcgctt tgcacacatc gtgcactccg
     1261 atggcgcaaa tcagaacggc ccacggcaga atcggaagaa gatgcgccgg atgcacgccc
     1321 aagggctcga acgaggcaaa cagcagggtg gtaaaaacca gacagacgag cgccaaggga
     1381 agccccgttt caaaactgcg ttgccgccgg aaaaccgatc gtgccacaag ccatccggcc
     1441 gaaacgagga acaaggtgta ataccccctc aaattgccga ccgaaaggtc ccagtaataa
     1501 ctcaggaagg tataccctcc ctccagaacc gcgcggaaaa tggcgaaact cctctgcggc
     1561 tgaaaaacca gcagagaaga cagccccact ccctgggaat gccccgacca caaaacaatc
     1621 aggtattcga tcggctggaa aaacggggga tcggaaaaaa gcatggcgag gaacaggggc
     1681 cccactccca aaagaaaaca gacgccgccc ccgaggacat ggctcttcca ggctgccatc
     1741 agcctccgga aaaaaccccg cccgaaacag aggagcgtgg ccgacagccc ggccatccaa
     1801 tagatgaacc agctcagcgt ccccaggcca agccccaggg taaaacacat gagaaaaaaa
     1861 tcttttgttc gaccgccttt ccaccaacgg atcaaaaagc tgacagcaag gacggaaaag
     1921 aggagaagca ggttcccgtc ccggacaccc atccgcgtta taagcaaaaa ggccggatga
     1981 aacgcgagga acgacaaaga aagcagggcg gccccctccc cataaaggtc gaacaccaac
     2041 cgatagagac acagcaaggt caatacactg taaaggacca tggccagacg catggcgacg
     2101 accggatcgg atatgatttt cagaaacggg tagacggagt aggcgatcca agtgccgtgg
     2161 tggccgtcga actgcaacgg aagccgttca aaggagaggt tttttaaagc gtgcagaaaa
     2221 tcgatgccgg cggcggcgta gcccatggag gcctcgtcac ggccgatgcc cgggcttttc
     2281 aagtcccaca acgcaaaaag gaggaacaag accagggcgc caaaaagagc ggccttatga
     2341 ccccgtgacg tcctcaacgc gaagagattg agatatctaa aggcgccgcc gacggcggcg
     2401 gtgatcgcat gaaacgcggg ggcagccaat gatttcattc tctatccgat cattttcctg
     2461 ggaagcttcc ttgaagacaa gttattccaa gccgacgaca ttatcgcaaa tttttaaaag
     2521 ctcgtcgggg gctgtccaca aaacccgccg agaggccccc gccaacaaga acaaagcccg
     2581 tcgcgcccgg agcccggccg ccccccggca cgccctttcc caggcgggtc ccacgaccga
     2641 aaacttcttc aagaaaccgg agcggcttcg gtccccgaga agcgatcctc cgggcgaaga
     2701 aaggaaacga cgccgtattc gccgtcatac cccggccgaa tgtccgcttt tccctcacgc
     2761 atccgcgtga ggaccaatgc caaaagagaa aaaccctccg cctccagccg acccggcggc
     2821 agttcccgca aaacaaacaa ctcgtttccg aagagggaca acagccggtg ataagcggca
     2881 tccactttcc ggctgcccgg accgacgtcg agggtttcgg acaggagctc ctttagcgga
     2941 acgaggctct cataggccga ggccgacgac ggttttttcc ccgcctcccg gtcggccagc
     3001 ttctccacgc ggctcaatac gccgacgatg acgggcttgg cgcagacggg acaaagtcct
     3061 cccttttctt gcgtctcttt cggcgtcagc cgggtttggc atttgcgatg gccgtccaag
     3121 tggtatttgc cttcctcggg aaaaaattcg aaggtggcgg tgagccggcc ccccgggcga
     3181 cgccgaagcg catcgaaaat gccttcgtag gagaacggag catcgaagaa attcgcctcc
     3241 cgggcgatct tttccggaga atgggcgtcg gaattggaga tcaggctgag ccgatccaat
     3301 ccggaaacgc gccggttcat cggcgggtcg gaagaaagcc cggtctccag ggcgaagatc
     3361 tccggtgcca attcgtcgaa acactcttcc agcgaatcga acccggactc cgacccgaaa
     3421 accgcgaagt gcggcgtcca cgcgtgggcg gggatgaggt aggcgcggtc ggaaacgtcc
     3481 cgcacccgct tcaaaaggtc tttggaatcc aaccccagga tgggtcggcc gtcggagcgc
     3541 agattcccga ttttttccag gctttccctg agccgcccgg cgacttcgaa cgaaggagcg
     3601 tagatcaggt gatggacttt ccttacgcgg ccggcctttt tgtagataca gcttatttcc
     3661 acttccaata aaaaccggac gggcgcgcga caggtcggcg gaatttcccc gttgaccttt
     3721 tccgccagtt ccggcttcaa ctcgaacagc ccctcctccg ccggccgcag tttatcgcgc
     3781 agttcggcga accaggcggg atgggtgaaa tcgccgctcc ccaggacggc gacccctttc
     3841 agttgacacc accggtatag agattccggc cggaggtcct tgctgcaggc ccgggaatac
     3901 ggcgaatgga tgtggagatc ggcgacgaat ttggtcatgg atcggtctcc cttgcggcag
     3961 aatcctccgg aggttttttc tgagttcgac tttcctattt tacgccaaac gggacgtctc
     4021 gaacagccgg attcgttccc ccttattgac acccccccca gccggtgcta tcatccggcc
     4081 atagctgaat tcctcgccgc tccccctttt gggaaaagcg tccgaggagg cgggggaacc
     4141 aagaagtcgg acctctgttc cggtcctgag gcgaatctcc gaaaggggta gggcgaaaag
     4201 cccgagtctg acagctcacc tcgtaagccc acagcataga gcacaagcca aagagcccgc
     4261 tctttggttt tttttatgcc aaggggcctt ccttcagcat caggaggctt atcatgtggc
     4321 agcgaatcaa aggctgggtt ctggggaaag aaaaaaatcc ccaggacccg gaggtctttc
     4381 ataaggtatc cctcatcgcg ttcttcgcct gggtcggtct tggcgcggac ggactttcct
     4441 cttccgccta cggtccggag gaggcctatc gcgccctcgg cgcacacacc cacctggcca
     4501 tcctcctggt ggccatgacg gcaatcacca tcttcgtcat ctccatcgcc tactccaacc
     4561 ttatccaaca ttttcccggc ggcggcggag ggtatctggt ggccacaaaa ctccttgggc
     4621 cgaaggtcgg ggtcatttcc ggctgtgcct tgttggtgga ctacgtgctc accatttccg
     4681 tctccatcgc ttccggctgc gacgcacttt ggtcgttttt cccggccggg gcggcccctt
     4741 tcaagctgac gggcgcaata atcctcttgt tccttctgtt ggcactgaat ttacgcggga
     4801 tcaaggaatc cgtcaccgta ttggccccca tcttcctgct cttcatcgtg acacatgtct
     4861 tcatgatcgt ttacgccgtg acgtcccatt ttggcggatt gccccgaata tttcatggag
     4921 cggcggccga cttccattcc tccgtctcat caatgggttt cctgccggtg gccctcatcc
     4981 tgcttcgcgc ctactcgatg ggaggcggga cctacaccgg catcgaggcc gtctccaacg
     5041 gcgtcgccat cctgagggaa ccccgggttc aaaccgggaa aaaaaccatg ttcttgatgg
     5101 ccacctccct cgcctttacc gccggaggca tcctgttcgc ctacttgctc accgacgccc
     5161 ggccggtgtt cgggaaaacg atgaacgcgg tccttttggg aaatcttttc gggtcgtggg
     5221 aatggcatgg gctgaacatc gggtatgatt tcgtcgtcat cgccctcgtc tccgaagccg
     5281 ccctcctgct ggttgcggca caagcgggct ttcttgacgg tccgcgggtc ttggccaaca
     5341 tggcttctga ttcttggata ccccaccgct tctctcaact gtccgaccga ctggtgacac
     5401 aaaacggggt tttgctcatg ggcggcgcgg cgctggccac cctgtactat acgaacggaa
     5461 acatcaccac gctggttgtg atgtattcca tcaatgtctt catcaccttc tccctcacag
     5521 aactcggcat ggcccgccat tggatcgtgc ggcgccgcac ggaaccgaaa tggaagagcc
     5581 aattgaccgt ccatggcaca gggctcgtca tgtgcctcag catccctccg ttactcttta
     5641 cgaaaaattc tcggagggcg ggtgggtaac cctctcggtc accggagtgg tgatcgtctc
     5701 ctgtttcctc atccaccgac actacgaaaa aacaaggagc gcgctccggc gactcgaaga
     5761 catcctcccc cgaatgccgg agaaagcgtc tgccaaaatc cccgaaaaat ccttccgact
     5821 tccagcggcc ctgttgatgg tgaacggata caacggtttc ggcatccatt cttttctggc
     5881 catccaccgt ttcttccccg gccacttcaa gaacttcgtc ttcctgtcgg tcggcgctgt
     5941 ggacagcagc cgctttaaag gcgcagaaga actgcagaaa ctcaaagaat ccgtgtcggc
     6001 cgatctggaa aaatacgtca agttggccaa cgggatgagc ctgtatgccg aatcccacat
     6061 gcttctggaa accgacgtgg tggaagggct ggaaacactg tgccaccggg aaggggctca
     6121 ttggcccaaa aagagctact tcatgggcca gttggtcttt gaggcggaca atttctggaa
     6181 ccgtttcctc cacaaccaaa cgtccttcac ccttcaacgc cgcctcattt tcagaggcta
     6241 tgaagtggtg gttctgccga tacgggttcg ccttcaacag acacgcttac cgggctaatg
     6301 gtctgggaag tgcgcgtttc cgttttctgc ctccggcggc gggcttgata atcgttctct
     6361 aaacaattaa ggtacccgca tgaacgcgct gccgtccgtc ggattcatcg ggctggggat
     6421 catgggcctg cccatggcga gacacctcct cgccggggga taccccgtca cggttttcaa
     6481 ccgcacggcc tccaaggcca agacgttgga gtccgtcggc gcgaaagtcg cggcccatcc
     6541 caaagaaaca gcctcccgat gtcgggttct catcaccatg ctgaccgatc cggcggccat
     6601 ccggcaagtg atggacgggc cggacgggtt cctggcggcc gacaacccgg acgtcgtctg
     6661 gatacagatg agcaccctgg acatcgcgtc caccatggac tttgccgcaa cagcgaaaaa
     6721 gaaggggtgg gggtttttgg attgcccggt cacgggcagc aagaagcagg tggaggcggc
     6781 ggaactgatc ctgctggcgg gcggaccggc cggtttgctc gaggcgcacc ggcccctttt
     6841 aatgaaaatg gcgaaggcca tcgtgcacgc cggagacgtg ggggccggct cggcccttaa
     6901 gctctgcatg aacctcatcg tggcgcagat gacgacgggg ctggcggagg cggcagcgct
     6961 ggccgatacc tgcggcatcg atcccgcccg cattttcgac gtgctgaagg aaagcccggc
     7021 actgaactgc gcctattacc aaataaaaaa ggagccggtc ctcaaaaaag atttttcccc
     7081 cgccttctcc ctggccaaca tgctcaaaga cgtccggttc atgaccaaag aagccggagg
     7141 gcgggggctg gacctgccgg tgacgcaggc cgtacgcgcc ctgatggaaa gcgccttaca
     7201 ggaaggccgc ggcgataagg acctgatgtc catttacctg acgctgcgcc gaaacaaaaa
     7261 aagcggctga tcgcttcccg ttcacctcgg accgtttctc cgggttttcc ctcaacactc
     7321 catcgaaatg cagcgcactc ccgcgccctc ttttccatcc gcccgtgcgt cgctctgccg
     7381 ctgcggcaga aaaaatcccg cggaaccggt cctcttcggc gatgaagcgg ggggtcggcg
     7441 aagaaaaagt ggtatttacg ccgaaacgcg ccggccggtg agcgcgaagg agccgccggc
     7501 caggtggtgg aaggtccggg cccgggcggt gagaaccccg ttcttataac acgatttttc
     7561 cgcggcggcg tgcaggactt tgccgacgaa gagatagacc ccgccgaagt ttcgtttcca
     7621 aagaagcgcg cattcgatga aagcgatggc gtctttcagg tgcggcgtgc ggactttcac
     7681 gcccctctcc agccgcagcc cggtcttggc gaatttgtcg cctttccggc cggacacgct
     7741 cccgcaaaat ttcagcggtt tcaacaattc gagcggcggc acgttgatga cgaattgttt
     7801 tttcctcagg atcagttccg tggtgtagtg cgtctccgcc accacgatcc ccgccagcgc
     7861 agggtcgtcg ttgaccgggg tcgtccaggc gatcggcgcc acgttgcccc tcttctcatc
     7921 cccggacgtg atcaggacgc acggaccgaa acccaacaga tgatagaact cccccaccgc
     7981 caagcgctta aacatagacc ctcccggtca aaaaatggct gctgtcccgc ctattgtatc
     8041 acgtcgggct ttctcttgtc gtccggcggc cctgtttgat tgttcatctt ggaacggcta
     8101 ttatttaacg attgaccctt ttatcccgtt cccccgggaa cggtcgtgcg ggcttggaaa
     8161 aggaaacggg gcacctgtct tgatgataaa aattttccct cctcaacggg gcgcggccct
     8221 tctgcttttg tggacggtcg cggtcggccc attccccctc accgccgcgg aggacccctc
     8281 cccgtcggga tccgttccca ctcttcgcga ttgttaccgg tttgccttga aaaaaagcga
     8341 ggccgtggcc ctgagcgcgg aggagatccg actggcggag gcgcaatacc tcgtggcccg
     8401 ctccggcgtc cttcctcagg tcgggttcaa cctcatcgag ttgttccagg acgagggaaa
     8461 cacctcctcc gactccatcc ggcgcactca aaccacggaa aaattctacg cgaaacaact
     8521 cctcttctcc ggcttccgcg aaatggccgc cctcaaaggc gtgaaggcgc agatcgccgg
     8581 acagaccttt gccctcgacc gggcgaaaca gctcctcttt gcggacgtgg cgctggcgtt
     8641 cttcaacgtg gcggacttcg accgccgcct cctcacgctg ggttcgatga agtccgccgc
     8701 ggcggaccgc ctgagtgaac tgcaggggcg ggaaaaaata ggccgctccc gccgcagcga
     8761 ggtgctttcg gcccagaccc aaatcgccac gctcgatgcg cggatcgagg aggcccggaa
     8821 actccgcgcc gccgcgctgg aactcctgaa cttcctgacg gggcaaccga tccgcgggat
     8881 cgccgacgac gtggccgacc ccgccccgcc gccgcccttg gaagattttc tggccggcgc
     8941 ctcctcccgg ccggacgtcc gcgcggccga tgaggaacgc cgggcggcgg aaagttttct
     9001 ctccgcggcg cggcgggcct cctggcccac cctgaacctt cagggcggtt actatgtccg
     9061 tcgggacggg acctacgagc ccgtcgattg ggacgcccag ctgacggcgg acctgcctct
     9121 tttcacgggc gggagcctga agggcggccg gagagcggcc ggcgcgcggg cgcgaacggc
     9181 ggccctcgcg ttctcgcgaa cgacgcgcca ggccgagagc gaggtgcgtt ccgcccatca
     9241 gagcctctcc ggcagcctct cccaaatcca aaagctggcg acggccctgg aactcgccga
     9301 aaaaaattat cgcgtgcaga cggaagaata ccgtctggga ctggtcaata atttggaggt
     9361 cttgtcgtcg ttgagttccc tgctggagac ccgtctggcc tacgatcagg ccctgttgaa
     9421 cgccaagctc gacgccgtcc ggcttgcggt ggccgcgggg aaagaacctc tgccatgaag
     9481 ctctccgacc tttccataaa aaaccccgtc ttcgcctgga tgctgatgtt cgccctgatg
     9541 cttttcggcg ccatcggatt ctcgcgcatg ggcgtgagcc aaatgcccga cgtggatttc
     9601 cccgtgctca acgtgcgcgt gacctgggaa ggcgcggccc cggaagtcgt ggaggccaac
     9661 gtggtggacg tgctcgagga cgcgatcatg tcggtggagg ggatccggga agtctcctcc
     9721 tccagccggc aggggaacgc ctccctcacc atcgagttcg agttgaaccg cgacatcgac
     9781 gccgccctcc aggaagtgca gaccaaagtc gcccaggcac agcgccgcct gccgcgcgac
     9841 atcgacccgc ccgtcatcac caagtccaac cccgaagacc agcccatcgt gtggatatcg
     9901 ctttcgggag accgccccgt caaagacctc atgctttacg ccgacgaaca tctgagggaa
     9961 aaattccaga cgatccccgg cgtgggcgag gtgttcatgg gaggtttcac ggcccggaac
    10021 ctccgtgtct gggtgaacgc cgacaaactg tcggccaaag agatgacggt ggacgacatc
    10081 ctctccgccg tcgccaacca gcacgcggag gtgcccgccg gcatcatgga caccggcccg
    10141 caggaactca acgtccgttc catgggggaa gccgcctccg tccaggaatt ccgcaaactc
    10201 atcctgactc aacggggggg ctcgccggtc tatgttccga tcaaactggg cgacgtggcc
    10261 gaggtggagg acgggctcga cgacatgcgc cgcatttcac gcgccatggg caagaccgcc
    10321 gtggggttgg gcatccgtaa acagcgcggg tccaacgccg tggccgtagc cgaccgtgtc
    10381 atcgcccgga tgaacgaact gaggccccag cttccccagg gcctggagtt ggaagtcaat
    10441 ttcgactcca ccaagttcat caaggacgcc gtccatgaac tcaacttcac cctggtgctt
    10501 tccgccctcc tcacctccct cgtctgctgg gcgttcctgg gttcctggag ttccaccgtc
    10561 aacgtccttc tggccatccc cacctcggtg ctgggcacct tcatcgtcct ctattttctg
    10621 ggcttcaccc tcaacatctt cacgctcctc ggcctctccc tcgccatcgg cgtggtggtg
    10681 gacgacgcca tcatgatgct ggagaacatc gtacgccacc gcgaacgcgg gcaggagcgg
    10741 gtcagcgccg cggtctacgg cgcgcgggag atctctttcg cggccatggc ggccaccgcc
    10801 gcggtggtgg ccatcttcgc gccggtggcg ttcatgaaag gaatcatcgg caaattcttt
    10861 ttccagttcg gcgtcaccat ctcggtggcc gtcctgctct cgctcctgga ggccctgacc
    10921 ctcaccccca tgcgttgttc gcagttcctc aacgtgcgtc cccgcaccac ctggttcgga
    10981 cacggcgtgg aggccgcgtt cctctggctc gcccgccggt atcgggaggt tctggaaatc
    11041 tgcctgcgct ggcgctggac ggtcatcctc ctgtccgttc ttttcttttc ggcctcgctc
    11101 tacgtttata aggggatccc aaaggagttc gtgccggccc aagaccaaag ccttttcctc
    11161 gtccgtctgc agaccccggt gggctcgtcc ttggaattca ccgacgaccg gttcaagaag
    11221 gcggaggcct acctcgccgc ccgtcccgag gtccggcgtt atttcggctc cgtgggcggg
    11281 ttcggtggcg gggaggtcaa ctcgggcatc atcttcgtca ccatgaggga acccaaggac
    11341 cggcctcgaa accccgagac cggcgagccc gtcacccagc aggaactcat gacgctctgc
    11401 cgcaaggccc tgaacgcgat ccccgacgtc cgtgccgtga tccaggaccc gtccacgcgc
    11461 ggattcaccg cccaacgcgg gttccccgtg gagttcacca tccgcggtcc ggactgggcc
    11521 gtactcgccg attcggtgaa aaaagtgatg gaccggatgg aggcgtccgg gctgatgctg
    11581 gacgtggaca ccgattacaa agtgggccaa cccgaactgc ggatctatcc gaaccgcgcg
    11641 gcggccgcgg cacggggagt ggacgtggcc gccatcggac ggaccatcaa cgcgttgatc
    11701 ggcggtgtcc gcgccggaca gttcaccgag tccggaaaac gctacgacat ccgcgtccgc
    11761 ctcgtcccct ccgaacgatc ccggccggag gacatcaacc ggctctatgt ccgcaacaac
    11821 cacggcgaga ggatccgcct gtccgaactc gtccgcctgg agcagacgcc caccttgcag
    11881 aacatcacgc ggcgccaacg ggaacgcgcc atcggcgtgt tcgccaacgt cgccccgggc
    11941 aaatcccagg aaaaagccct cgccgaggtg gagaaaatcg gcaaggaggt gctgcccgac
    12001 ggctaccacg tggtcatggg cggcggagcg aaaacgttcc aggaatcctt ccagagcctc
    12061 ttcttcgccc tgtggctggg cgtcatcgtg gcctatatgg tgctgggttc tcaattcaac
    12121 agtttccttc acccgttcac ggtgctgctg gccttgccgt tcagcgccac cggcgcgctg
    12181 ctggcgctgc gcttggccgg gctgtcgttc aacatctaca gcatgatcgg gttcatcctc
    12241 ctcatgggca tcgtgaaaaa gaactccatc ctcctggtgg acttcaccaa ccaggtccgc
    12301 gcccggggga aagacgtgca cggggcgctc ctcaccgcct gtcccatccg gctgaggccg
    12361 atcctcatga cgtccctttc caccatcgcc gcggcggtgc cgccggcgct ggcgctgggg
    12421 ccgggcgccg aaacccgcgt ccccatggcg gtggcggtca tcggcggcgt gtgcgtctcc
    12481 accctgctca cgctgttcgt cgtcccctgc gcctacagcc tcctgatccc catcgaacgc
    12541 cgccatccgc acaacatcct ggaaaccctc gaacccctcc tggaagaaca cgaccctccg
    12601 ccccaggccc cgcccaccgg ccgccgaccg ccggacggac gatagaaccc gtcgcctctt
    12661 cttctccagg aacggtcgcc cccgataatt tggtataatt tccccgccac tgattccgac
    12721 gcgccttcgg ttttccccca ttcccacttc catcattcgg gaaaaaaacg gcgacggtcc
    12781 cctcttgagg agaccgcccc cggcgcccta ttttcatgaa cagcaaaaag gacggttgaa
    12841 tgacaacgac ggcacatcac cggcgaaaaa atatccgaaa catcgccatc atcgcccacg
    12901 tcgaccacgg caagaccacg ctggtggacg ccatgttgcg gcaaaccggc gagttcaagg
    12961 tcaaacccgc cgaagcccag gaatgcgtgc tcgactccaa tccccaggaa cgggaacgcg
    13021 gcatcaccat cctgtccaaa tgcacctccg tcccctacaa ggacgtgctc atcaacatcg
    13081 tggacacccc cggccacgcc gatttcggca gcgaggtgga acgcatcctt tgcatggtgg
    13141 acggcgtgct cctgctggtg gacgccctgg acggccccat gccgcagacg cggttcgtcc
    13201 tgcgcaaatc cctcgagctc ggcctgcggc ccatcgtggt catcaacaag gtggaccgcc
    13261 ccttcgcgga cccgctcaag gccctcgacg gcacgttcaa tcttttcctg aacctcggcg
    13321 ccacggacgc ccagctcgat tttcccacgg tctacgcctc cggcaaagag ggctgggcct
    13381 ccctcgacca tgaaaaaccg gggaacacgc tcacccctct cttcgatgtc atcctcaaac
    13441 acgtgccggg ccccgtggcc aacgccgaca aacctctcca gatgcaggtg accatgctcg
    13501 actacaacag ctacgtcggc cgcatcggcg tgggccggct catgaacggc cgcgtgaccc
    13561 agggccagac cgtcgccctc ctcaaaaccg acggacgcac cttcaattat cgcgtcacca
    13621 agatgtacgg atatttcggg ttggagaaaa gggagatcgc caaagcggag gcgggcgaca
    13681 tcgtggcgct ggccgggctg gaagaggtac acgtggggga caccgtggcc tccgtggaat
    13741 cccccgaggc cctgcctccc atggagatcg acgagccgac cctctccatg gaattcatgg
    13801 taaacgaaag cccttttgcc ggacgggatg gaaaattcct cacctcgcgc cacctgcgcg
    13861 cccggctcga gcaggaaaaa cagacgaacg tcggcctgcg catcgaggaa ctggaccggg
    13921 ccagcgccaa acagggcgtg ttcaaggttt cgggccgggg agaactccat ctgtcggtgc
    13981 tgatcgagac catgcggcgc gaagggttcg agttggcggt gtcgcgcccc gaagtgatct
    14041 acaagaaccg agacggcgcg gtcctggaac cggcggaata tctggtgctc gacatcgacc
    14101 gtcaatacca gggcgcggcc atggaacagc tgggccggcg cggcgcggaa ataaaacaca
    14161 tgaccatgga gggcgaaaac accctgcgcc tggaatgcgt catcaccgcc cgcgccctca
    14221 tcggattcaa atcggaactg atgacccaaa ccaaaggcac cggcctcatg caccacagct
    14281 ttcacggcta cgtccccaag actgccgaac cgggccaccg cgccgccggc gtcctggtgg
    14341 ccaaggaaaa agggtccacc accgcctacg ccctggagtc cctgcaggaa cgggcggtga
    14401 tgttcgtcgg ccccggggtg gaggtctacg aaggaatgat cgtcggcatg aactcccggg
    14461 agaacgacat ggtcgtgaac ccctgcaaga aaaaagccct caccaacatg cgggccgccg
    14521 gatccgacga caccattcaa ctcacgcctc cgaaaatatt caccctggaa caggccatcg
    14581 agttcatcga aaccgacgaa ctggtggaaa tcacccccga caacatccgt cttcgaaaaa
    14641 aagagctgaa cttctcggcc cgccgaaaaa acgagcggga cgaatccgaa taattccctc
    14701 ctccctctaa aaacaacgtc gtttcttggg aactcccccg tcccctccct gttttattaa
    14761 aaaggacgcc ccgggcccct ccgccgtttc aggaacggac ggatccccgg cgatgggagg
    14821 aaacgatggc cgcaaaccgc cttgccgcgg aaatcagccc ctacctgatc caccatgccg
    14881 ataaccccgt ggattggtat ccgtgggggc cggaggcttt cgcccgcgcc aaaagagagg
    14941 acaagcccgt cttcctctcc gtcggctatt ccgcctgcca ctggtgccac gtgatggaac
    15001 gcgaatcttt tcaaaacgga tccatcgccg ccgtcttgaa cgaacacttc gtctgcatca
    15061 aggtggaccg ggaagaacgt ccggatgttg acgaatttta tatggcctcc gtccagcttt
    15121 tcgcgggccg gggcggctgg cccatgacgg tgttcctcac gccggacggc gagccctttt
    15181 tcggcggcac ctatttcccg cccgaaaacc gggcgggcca ggccgggctg aaagagcttc
    15241 tggaaggaat cgcccgggcc tatcgggacg aacgtccccg gatccggacc ggcgccgcag
    15301 cgatcgtcgc ccaactgaaa aagaatttcc ggccggcggc gcccgggcgg cccctctcct
    15361 cccatgggat cgaacgggcc gggcgggaac ttctcctcca gaaggatccc ctccacggcg
    15421 ggttcgggga cgccccgaaa ttcccccggt cctcggacct ccaattcctt atgcgctatt
    15481 ggcgaaaatc gggtgatcgg gacgccctgt cccacgtaca actgaccctg gacaaaatgg
    15541 ccgccggcgg catctatgac cacttggccg gcggatttca ccgctacagc gtgcacagcg
    15601 actggagcgc gccgcatttc gaaaaaatgc tttacgacaa cgccctcctc ccgcgcgcct
    15661 atctggacgc ctaccaggcc gcccctcatc cccgccacgc ccgggtggtg cgcgaaaccc
    15721 tggagtacgt cctccgggaa atgtccgatc ccgccggcgg gttccactcc agccaggacg
    15781 ccgacagcga aggccgggaa ggatcctatt acctctggac gctccgagag atacacgacg
    15841 ttctgggtcc ggaagacggg ccgctcgccg gcgactattt caacgtccgg gccggaggca
    15901 actttgaagc cgggcagaac gtcctccgga tcacggaaac cgacaatgcc ttctgcgccc
    15961 ggcaccggat cgatccgctc ctctggccgg agaggcgccg ttctttctgc cggaaacttc
    16021 tggagatccg ggaaaaacgc gtcaaaccgg acaaggacga taaaatcatc accgcctgga
    16081 acggtctcat gatcggcacg ctcgcccggg ccggcagaat cctccaagag cgctcctacg
    16141 aaactgccgc cacccgggcg gcgtatttcc ttctcaacaa catgttccac gaaggccgcc
    16201 tcttccgcgc ctggcgcggc ggccaggcga agtggaaagc gccgctggaa gactacgcct
    16261 ttttggcggc gggcctcttg gacctgtacg aggccacggg ccagacgcgc tgggccaagg
    16321 aagcgctggt gcttaacaac gtgatgttga aacttttctg ggacaaaacc cagggcggtt
    16381 ttttctttgt cggtgaggat gagaacggcg cgccggaccg gctgaaaacg gccggagacg
    16441 gcgtgcttcc gtcccccaac gccgtggccg ccatgaacct gctgcgcctg catgaattga
    16501 cgttggatcc ggaacttcgg gaaatggccc acaagaccct gcgccttttt tccggagact
    16561 cgggccgcgc cgggatcgga gacccctccc tcctggccgc cctggatttc catctggaac
    16621 ccccgctctc catcgtgctg gcgggcgaac cgcgcgaagc cgaccggcat ccgtttctgg
    16681 aaccgctgga ggcggcttat ttcccgaaca aagccctctc cttcgcccat gcgggggagg
    16741 aagcggagga aaaacttttc cccctcttga aagacaaacg gccggtggac ggaaaaccca
    16801 cggcctatgt ctgtcagaac gcggcctgtt ccgcacccat cacgacggtc caggcgctgc
    16861 tcgcccgcct tcaggagaaa tgacgtgcga acctccgccc gcctaaaaac cgtcctcctt
    16921 tcattcgccg tcacgccgac gctcctgtct tccttctttt tcttcgccca ggctcaggaa
    16981 cccaaccatc cccccaggag ggactccatg gaaaaataca cgaaaccatc tccggaaaca
    17041 ctgaagaaaa ggctcacgcc cctccaatac gacgtgactc aatgcagcct caccgaaccc
    17101 ccctttcaaa atgcctactg ggacttcaag gaagcgggca tttacgtgga cgcggtcagc
    17161 ggcgaacccc tgttcagttc cctggacaaa ttcgactccg gcaccggctg gccgagcttc
    17221 accaaaccct tggccgatga aaacataacg gaacaggcgg acctttccca cggcatggtg
    17281 cgccgcgagg tgcgttcgaa acacggcgat tcccatctgg gacacctttt ccccgacggg
    17341 ccaggaccca ccggccggcg ctattgcatc aattccgcct cgctccgttt catcccgacg
    17401 gccgatctcg aaaaaacggg gtacggggaa tatcgctccc tcttcgccaa atccgggaaa
    17461 ccggagaaac cggcgccgac gtccagactt gaaaccgcca ctctggccgg cggatgcttt
    17521 tggggcgtcg aggacatttt cgccaaaatc cccggcgtgg cgtccaccga ggtgggctac
    17581 gccgggggcg acagggaccg tcccagctac gaaagcgtgt ccgccgggaa caccggccac
    17641 gccgaaacgg tccaggtggt gttcgatccc accaaaatca cgtatgcgga gatcctttcc
    17701 tatttcttcc gtctgcacga tcccaccacc ccggaccgcc agggaccgga catcggttcc
    17761 cagtaccgct ccgcgatctt ctatcacacg ccggaacaga aacggaccgc ggaggaggtc
    17821 aaaaagatgg tggccgattc gggccgctgg aaggatccgg tggcgaccca gattgcgccg
    17881 ttcaaggcgt ttcaccgggc cgaggaatac caccaaaaat acctctccaa aaacccggac
    17941 ggctaccact gtcccacgca tttcctgcgc aagccgtata aaaaataagt ccgaccggac
    18001 aaaaacgtcc ggccggtgcc ccgagcggcg ctcggtccgg ttatttcagc gggggtgtct
    18061 tttgtggagg gagggcggcc gcccgggcgc gatgttgcgc caggatgctt cggtgacggc
    18121 cgtagctgac gtaaatcacg aagccgatca aaagccaaac caacaggcgc aaccagtttt
    18181 ccgtcggaag ggaaaacatg agcaacagac aggtggcgat gcccgcaaaa gggacccacg
    18241 ggacccacgg gcagcggaac ggccgcggcg catccggatc cgtcttccgc atgatcagca
    18301 ccgccgcgca gacgatgagg aaagccagga gcgttccgat gttgacgagt tcggcgagga
    18361 cccgcagcgg gatgaacgcc gccagcagcg ccaccacgcc gccggtcagg atggtggatt
    18421 tccacggcgt gcggtaccgc ggatgcaccg cgccgaacac gcccgacggc accatgccgt
    18481 cccgcgccat ggccaggaag acgcgcggct ggctcagcat cagcaccagc aaaacggagg
    18541 tgatgccggc catggcgccc agcgaaatga tgaactgcgc ccacggaagc cccacggcgc
    18601 ggaacgcctc cgccaccggc gcgtcgatgt tgatctggtc gtacggaacc atccccgtga
    18661 ggatcgcgga caccgcgatg taaaggatcg tgcagatcac cagcgacgcg atgatgccga
    18721 tgggcacgtc gcgctgcgga ttgcgcgcct cttccgcatg cgtcgaaacc gaatcgaacc
    18781 cgatgtaggc gaaaaagatc atgcccgccc ccgccatcat ccccaacggt tcccccccca
    18841 gccccgccgg accgatcaat gtcttgccga aaaaactgat gccgctgaac ccgaacgggg
    18901 cgaacgggga ccagttgtcg ggattgacgt agaacgcccc caccacgatc acgaacaaca
    18961 ccaccgccac cttgaccacg accatggccg tgttgaaaga ggcgctctcc ttgatgccga
    19021 tgaccaaaat gatggtgatg gccgcggtga tgacgatggc cggcacgtcc atcaccgtgc
    19081 ccgtggagac cagccggccc agctccggac tgaaatcgaa cggcgcccgg gaaagggcct
    19141 cgggaatctt taggcggaat atcccgatga agttctggaa atagtgcgac catccgtgcg
    19201 ccaccgtggc cgaggccacc gtgtattcaa gcaccaggtc ccaaccgatg atccaggcga
    19261 aaagttcccc cagcgtggca taggcgtagg tataggcgga cccggccacg ggagccaagg
    19321 aggcgaattc ggcgtaacac aaagcggaaa aaatacaggc caaccccgcc accaggaacg
    19381 aaacgatcag cgccgggccg gccttgtcgt gcgccgcaat ccccgtcaac acgaagatcc
    19441 ccgtaccgat gatgcagccg atccccaaac tggtcaactg gaccggcccc aggacgcggc
    19501 gcaggcggtt ctcgcccttc atctcttcca tcaacaggct gagcggtttc ttggcgcgca
    19561 ggtgtttcat gcctcttccc gtcctcttct cattctcatc accggtgtat tctacatttt
    19621 ccagtgggcg tttcaatccg ccgagcgcgg ccccgccgtc tcagtccttg ccccgcaccc
    19681 gatagcgcat ctgcatcacg ccgttttcaa actcctgcgt ccgcatcaac cgcaaggaga
    19741 cggccgtctt cagggatccg aaaagacgga tcccctcgcc caacaggacc gggaccacgc
    19801 tcacaatcag ttcgtccaaa aagtcctcct gcaggaaagc cccggccaac gcgccgccgc
    19861 ccaccagcca aatgttcttc tccgacaccc gccgaagagg cgggacccat ttctggaaat
    19921 ccccggattg gaaaacgacc cgcggatcga accttggcag cggacggctc gtcaccacgt
    19981 ggaccggctt tccggaatac ggccaagcgc ccaaactcgg gatctgttcg taggtgcgtg
    20041 cgccaaggac cagtctctcc accgaatcat aaaattcccg gtacccgtaa tcctctcccg
    20101 ttccctcaaa gggccggagc caggaaatgt cgccgttcgg ccgggcgatg aaaccgtcca
    20161 gactggaggc gacgtagtaa acgatttttg ccatatgccc ctccttcttc tctaactcca
    20221 acaacgcccc cttccggttt gtttccagag aacccgcccc ccatctcctc cgtcaaacgg
    20281 ttgaatcgtc cttgctgacg gaagcggtgt cttccaaatc cgatcggagc ctcctcccgt
    20341 ttttttaagt cgctttcgga actttaccct tccccttctg ttggaaacga gattggagaa
    20401 ctggtgtcat tatgaaaaat tcgtttttct tcggtctttt ttcctggaag cttcccctcc
    20461 ccggacgggc ggcgttccgc atcttaaagc tggcagcgct gttttatttt ttcccgctcg
    20521 tttccttcgc ggcgatgttg tcgccacagg tctcccaatt cattcactcg aacgatccat
    20581 ccaagaaacc catcatcgtc atctataaaa gagacaattc tttccggaaa gccgatcccc
    20641 gcgtctccgc ctctcccgcg gcggtcctcc gggccgaagc cgacggaacg cgccccgccc
    20701 cttccgcgct tcccgtctcc cccgaaccgc ggggccccgt ggacttgtgg ctcctgaacg
    20761 gctacgccgt tgaggccacg ccggaggaaa cgcggcgatt ggccgccgac ccgcgcgtgg
    20821 aatctctcca tgaaaatttt caattgcgcc tgccggacat cctcccccaa aaccatcgga
    20881 cggaaaacgt ggaaggcaat tggggactgg aaaagtccgg ggccaagatg gcccgggaga
    20941 tgttcggtgt caccggggcc ggcgtgcgca tcggccacct ggacaccggg ctggacgcct
    21001 cccatccggc gctgcaggga aaactcgcgg catgggccga gttcgatgcc gacggccggc
    21061 cggtgccggg ttctcaaccg cacgccgccg ggttccacgg gactcacacg gcgggcatat
    21121 tggtgggcaa caccgccggc ggcgaaccgc tgggggtggc gcctgacgcc aaacttctga
    21181 gcgcgctcgt cttgaacagc gacggcggga cttttcttca agtcatcgcc ggcatgcagt
    21241 gggccctcga ccccgacggc aatctctcga cggacgacgg cgcgcgcctc ctcaacatgt
    21301 ccctcggcgc caacgggcat tacgccgtct tcgatgccgt tgtggaccgc ctgctggaag
    21361 tgggggtgct tcctgttttt gccatcggca acggcggccc cggccaaacg acctccccgg
    21421 gcaacgtccg aggcgtcctg gcggtcggcg ccaccaccga agaagacacg gtggcgtatt
    21481 tttcaggcgg cgaggaaaaa atgggggcgg gccagcccct gttgaaaccg gaaatcagcg
    21541 cccccggcag cggcatccgt tccaccatgc cgggagggac cttcgcctcc tacagcggca
    21601 cgtccatggc gacaccgcat gtggccgggg ccgccgccct cctctgggag gcgaacccct
    21661 ccatgacggc ctctcaggtg aaagatcttt tgatgcaaac ctcggtggac atcgaacggc
    21721 cgggaccgga cgaacgggcc gggtcgggcc gcctggacgt cctggccgcc ctctcccgcc
    21781 tccgtgggat tcccctcctc cgggtcgcca ccaacggacc gcatggtccc gtccccgccg
    21841 tcgtgcgtat ccacaacacc ctgggctcaa acctccgcgt cgtccatgtg agtccgaaga
    21901 ccggcgtctt ctgcacggcc ctgccggaag gagattacac ggtggaggcg gcggtggggc
    21961 tcgactcgcc ggccagacta cccgtgcgga tggagccggg aaaggtcaaa gacttgaaat
    22021 tcgtcctcaa cgacgccccg aagaccctcg gcgtgctgtt ggtgtatccc aaccctttca
    22081 aaccgtatga aagacacgag gcggtgacct tcgcgggatt gcccgtcgac agccgcgtgc
    22141 agatctacac gctttccggc gcgttggttc gggagagcgg gagtctgcct gtcaacgaat
    22201 acgtctggga cgggcgcaac ggcgatgggg aacccgtggc cagcggcgtc tactattatg
    22261 tggccagcca ttacgacatc cgctccggct ggacgcgcaa aaaaggccag ttggtggtca
    22321 tccgatgagc ggaaggcaag gacgttccct cgtgatgcgc ttgctcctgg tggcggaaat
    22381 gtatcccacc ggcacggtct cggccggagc gggggccggc acaaccgccg ccgatttttt
    22441 attgaccccc ccggactccc gctcggcggc cctggggaac agcggcgtgg ccgatgcgga
    22501 atcgccgttt tcgctctaca acaacccggc tctcctcagc gacgccaaat ctccattgac
    22561 cctcgcggga ggcgtcgcgg aatggcctct gggcctacag gcgcagcact atgccgtttc
    22621 ctccgtctgg ggaacgccgg cggacggccc gtggggattg ggaatatttc tcagccagtg
    22681 gcaaaccgcc cccatccaag ccaccgacat ttacgggaac gcgaacggac gcgtccgcta
    22741 cacggcgcgc aacgtcggcc tgggactggc ccacgccctt tcccccgccc tgcacctggg
    22801 gggagccgtg aaaacgatat cccagcgatt cgaggagacg ggccgggccg gcggcaatac
    22861 ggggaccggc cacagcgccc tcgcctggga cttcggcgtc gcctacgaca cgcccctgaa
    22921 atacgtgcgc gtcgccgccg ccctgcagaa caccgggaac gacgtcgcgt tccggcagga
    22981 tcaggaaaaa ctgccgcgag ccctccgcct ggggatgcag ggcgctttct tcaaccgtca
    23041 gttccaatgg gccgccgagg gacggaagct ctccggaggg gcgctggccg tcaagggcgg
    23101 gatggggttc cggccgtttc ccaacatgat cctgcggatg ggattcgatt cgggactgcg
    23161 ggacattttc ctgggagtca ccaccggcgt ggggtttcgc ctgcgcaacg tcgacatcga
    23221 ttatgctttc gtccccctgg gaaacctggg gaccacgcaa cggatgacgc tggcctggcg
    23281 tatcggaaaa accgcccccc ggacccggaa cgccgagcgg cccccatcgc tctggtcggc
    23341 gaactgaccg gccgaaacgc cggcggactc cccccctcac atttcctata atctcctcct
    23401 atggaaatgt ccgagttcga ggatatcatc gaagccaccg tcaaaaaaat gccggcgcgg
    23461 ttccggaaaa tccttaaaaa cgaaggcatc cggctgatcg cccggggaaa aacgcccggc
    23521 gtgcttcagg accggtatcg gggggattgg gtgttcggcg tctttttggg cgtcccctat
    23581 aaccaacggt ccgttttcaa cgtcgaacag gagcccacgc gcatcgagct ctatcgggag
    23641 agttttgaaa aagccttccg ccggcggcgg gaaatggaag aacagatcgc ccgaacggtc
    23701 atccacgaaa tcggccatta cttcggcttc tccgaaaaag acctgagacg ccaccgactc
    23761 taaaatcccc tcctccccgc cctccggtcc cgccaggacg gtggacatcc accccccttt
    23821 gcgttaagat atggggccgt gctcaaatcc caattcgggg cgcggtcatc gacgccgccc
    23881 cccgtccgga ggtcccatga aaacaaaaat gaaatcctcg acacccgccc cttcgagcca
    23941 cagcgccccc gtcaacgcct gggacatggc gctggaacaa ctggacaccg tggccaaaca
    24001 catccgcctg gaccggggga tccacgaatt tctgcgttat cccaaaacga tattgtccgt
    24061 gtcggtcccc gtccgcatgg acaacggcac cgtccaagtt ttcgaaggcc accgcgtcca
    24121 acacaacgtg acccgcggcc cggccaaggg cggcatccgg ttccatcccg atctcacgct
    24181 ggacgaggtc aaggcgctgg ctttttggat gacgttgaaa tgcgccgtca tgggcctccc
    24241 ttacggcggc gccaaggggg gcgtccgcgt ggaccccaaa aaactgaccc tcggcgaact
    24301 ggaacggctc acgcgccggt tcgcgtcgga aatcgccatc atcatcggcc cggaaaaaga
    24361 catcccggca cccgacgtcg ccaccaaccc ccagatcatg gcgtggatca tggacaccta
    24421 ctccatgggg atcggctaca gcgcgccggg cgtggtcacc ggcaaaccgc tggagatcgg
    24481 cggttccctc ggccggaacg aggccacggg ccgcggcgtg gtggtgatca tcgaagaggc
    24541 tttccgccgc ctcaaatggc cgttgcgcgg ggccaccgcg gccgtccagg gattcggaaa
    24601 cgtcggctcc gtctgcgcca aactgctggc cggcatgggc gtgaaagtca tcgcggtgtc
    24661 cgacgtggac ggcggggttt actcggaaag ggggctggac atcgaaaaaa tgctggcggc
    24721 caaggccaaa ggcgccgggg tgaaggattt tcatgccccg ggaaccaccc gcatcaccaa
    24781 cgaagaactc ctcgaatgcc gctgcgatgt tctgatcccc gcggccctcg agaaccagat
    24841 cacgaaggac aacgctcccc gcttgaaagc gcggatggtt gccgaggccg ccaacggccc
    24901 caccaccccc gaagcggacc ggatcctgct gaaaaagaaa atccccatcc tgccggacat
    24961 cctcaccaac gccggcggcg tgacggtttc ttatttcgaa tgggtccagg acatccagtc
    25021 gttcttctgg aacgaggacg acgtcaacaa acggctgaaa gagatcatga tgggcgcttt
    25081 caacaacgtg ttccaggaag cccaaacagg gaatatcgac ctgcgcctgg cggcgcaggt
    25141 ggtggcggtg cggcggctgg cggaagccct gaaaatccgc ggggtctatc cgtaaaaacc
    25201 gcccgcccgt cacgctcgcc gtcgaacccg gccctcccgc gccccatttg aatcggggcg
    25261 tcctccgccc ccgcgtcttt cggttatttc cggaaaacgc cctcgtctct ccgtccccac
    25321 gcctctcctc gggaacttta tcggacctcg gtttgttgta ttcactccga agagcttaga
    25381 ggaggtctgt catgtccaat tcattgtatg tcgtggtggg ggccaccggg cacattggga
    25441 aggaaatcgt tttgggcctg ttggcaaaag ggaaaaaagt ccgggcctgg gcgcggacgg
    25501 cgtccaaatt ggaagagttg gccgcgcggg gagccgaaac ccttgagatc gacctgcaag
    25561 acggcgcggc cgtgaagaaa ggattcgacg gcgccaccgc cgctttcgtg atgatccccc
    25621 ccaattacac cgcgccgaac tttcgtgact ttcagaaccg gatcgggcaa gccctcgcgg
    25681 acggcatccg ggcgtccggg atcaagcatg tggtcaacct cagcagcatc ggcgcccatc
    25741 tgccggacaa gaccggcccc atcgcggggc tctacgacca ggaacagcgg ctcaacagat
    25801 taagcgacgt ccacatcgtc catcttcggc cggcgttctt catggagaac ctcctcggaa
    25861 acatccccgc cctcctccag atgaacgtcc tcggctcggc gatgaaaaaa gacctggcgt
    25921 tcccccacat cgccacgcgg gacatcgccg ccgtggccgt ggacttgctg gataaattgg
    25981 aatttacggg caagcaggtc cgcgagctcc tgggcccccg ggacgtctcc atgggcgagg
    26041 tcgcccgcgt cttcggcgcc gccatcggga aaccggacct cccctacgtc caattttcct
    26101 attccgacgt ggaaaaggcc ttcctccaca tggggatgtc gcccgacgcg gtccgaacgt
    26161 tcatcgaaat ggcccgggga ttcaacgagg ggctcgtcgt cgccacccaa ccccggtccg
    26221 ccctcacgaa cacgccgacg gacatcgaac aattcgcctc cgtttttgcc gccgcctaca
    26281 aagcggaacg ggagtcgcgg gccgtcgccg cccgatgacc ccgacgaggc gcaagcggga
    26341 gattgccgtt acggcttttt tgaaacgatc cgcccgtcgt aaccgatgac atagccgggg
    26401 ccgtcgtacg tctttttctt tttgtggacg aaatccacgt gccatccgtt cggccgcgga
    26461 tgtgccagaa tttcataccg ggccaagctc ccaacggctt ggcccgcgtc ttttcgggcg
    26521 atttccaccg cctcctcctt cgaaatcgct tccccggccg gcgagacgac caggaacccg
    26581 tccggcagtt tcccctgctt ttccgagtgt ttcaactcca atagatacct cacgtgaccg
    26641 accacgaacg agctcggggc gatcagcccc gatccggaag accacaaaat ctcctgttga
    26701 gcctcccggg cttggacatg gaaccgatac accgtcatct tccatggggc gttgttgggc
    26761 cccggaactt cgcggaggcc gacgtattgc agcgagaaat cattgaacgt gagcctcgga
    26821 tgagtcttcg ataaaccggc gtaggcgacg ctctgtccgt acggggcggg gcgatccgag
    26881 gatttttcgc cggcaagatt cgtcattccc gaacccagcg cgaagacggc tccgcaaacg
    26941 accgcggagg aaaagcggcg tccggaccgg cgatccctct gaaatcccgc ccggcgatcc
    27001 ggggaacgac ccccccgccc cgacccgccg tcgtctccag acagactcat ttcgtccccc
    27061 gttttccgcc gtggctgaaa aaccggcaaa cgacgaccgt tccctggggc acattcttcc
    27121 ggatggccag gttcccgccg atctccttgg cccgatactg gaggatcttc agtccgagcc
    27181 ctccctccgt tttttgtgga tcgaagccct ttccgttgtc ggcgacgcgc acctcgactt
    27241 ccgaagaacg cgacgtcaat aaaatcttta tgcggctggc ccggccatgc cttatcgcgt
    27301 tatggatgga ctcctgcacg atacggtaaa gatgcatgga aacgatgtcg tttttccggg
    27361 aaaccttccc gttgaccttc aacgaacatg ccacgttata gatgaccgcg gtctgcccgg
    27421 cgaggttctt cagggtcggc accaaccctt cctcttcgag ttccgccgga taaagcatat
    27481 gggaaagact cttggtctgc gagatgccgt tgtttatgca ttccaggatg cgcgccgttt
    27541 ctttggcggc gggcggtttc ccccttttaa ggtcgtgttc cacccctttg accctgtagg
    27601 cgatgccggt caggagc
//
LOCUS       DAOTFD010000013        26606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1189_length_26606_cov_4.558661, whole genome shotgun sequence.
ACCESSION   DAOTFD010000013 DAOTFD010000000
VERSION     DAOTFD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_26606_cov_4.558661"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..441
                     /locus_tag="P5079_01670"
     CDS             133..441
                     /locus_tag="P5079_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY28727.1"
                     /translation="MPAPADALRTTLRAALILAAFVAIVAANSLVKIGGLSLTPLLTA
                     TRSPDVNVRVVSCVALGDLGDPSAVPALERCAADEAGDVRSAAQTALNKIRSSTSASR
                     "
     gene            complement(438..1337)
                     /locus_tag="P5079_01675"
     CDS             complement(438..1337)
                     /locus_tag="P5079_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28728.1"
                     /translation="MKTLKRALLAGGVPLLFSVFGAANEIEYQLDAYYTSVDYYVNLT
                     QSPIPYHEFKSETDIYKKLILSSPIPRFLLLETSVNPLPLLGVYLKRQARDFYDDTQF
                     SEDSNLIRSITTGFEEPAAVSLFFGNIVDFKPVKKRDYAEGRGHIGYLVSAGNYHIKD
                     NDMMKDDWVETEWKVKGDRIYPDMELRWSFRIGAKFHARKEITDVYYLGVRRSRIHFK
                     ERGFSWLNNSGIMYKFDFDQSRLSAVQHHFVVDKKFPLPDKKVVPSLALGFLWRSEKK
                     YTGDLGDARRTVFQFLFQPNIEF"
     gene            complement(1431..2795)
                     /locus_tag="P5079_01680"
     CDS             complement(1431..2795)
                     /locus_tag="P5079_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRY28729.1"
                     /translation="MSDPRIPEYLKAVETLKDGSFQIELPCGFDDDIGRLGRALVDLG
                     PAMEKKLGEIHTLLKITEKISSGRSLEEILNLVYDSFRAVIPYDRIGVALIEEAGATV
                     RTRWGRSETSPQFFPPDYSAPLKNSSLQKVAETGRPRIINDLESYLSSHPESESTRLI
                     LKEGVRSSLTCPLIAEGKAIGFLFFSSRQRDTYRNPHADIFLLIAGLISEIIEKGRLH
                     DSLLALNEQRSRILGMVVHDLRQPLSVIWGYLSYATDGRGVTRTETLSQLMRLAKKSC
                     NSMIYLINDLLDIHSIEQGQLALVLQETDPAEFLKDFHMTHLPITQAADFEFTLTLDP
                     DLPKVRMDANRIGQVLENLLSNAIKYSPFRTTISLRAENRENEAWISVADQGIGIPAK
                     EIPSLFTEFGRGSAQPLHDEKSTGLGLAICKKLVQVHGGRIWVESAPGKGATFIFSLP
                     ALSS"
     gene            complement(3194..3279)
                     /locus_tag="P5079_01685"
     tRNA            complement(3194..3279)
                     /locus_tag="P5079_01685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3243..3245),aa:Leu,seq:caa)
     gene            3353..4198
                     /locus_tag="P5079_01690"
     CDS             3353..4198
                     /locus_tag="P5079_01690"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRY28730.1"
                     /translation="MRIGVHCSVRNGFVGALREAAALGCETLQIFTQSPRGWRTRVYE
                     PTEFSDFRRERERLGLGPVVVHSPYLPNLCTSDAALYARSVRALREDLDRCEKLGAEY
                     LVLHPGAYSPSADYNRGVERLSSALDAVLGAAGEGTRLLVENMAGGGRRIGSSFREIA
                     DIFSNVRHRSRLGVCFDTCHALAAGYDLTSASGIEKTFEEFDRALGLKQIRAFHVNDS
                     KTLRGSRRDIHQHIGRGIIGVEGFRRIFSRRIFFGLPLILETPKDSPRADRRNLRVLR
                     RCLPE"
     gene            4311..4964
                     /locus_tag="P5079_01695"
     CDS             4311..4964
                     /locus_tag="P5079_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRY28731.1"
                     /translation="MSKKSHFKGYFLTGLVALLPLWVTIILLRALFYIISNATRPILQ
                     PILREWTPLEYAPFLFDLTCFLGTLLIVYLVGVLVSNLVGRQIFVKVERLIFRLPIVA
                     DVYQAVRKLTDMFSSNANSKFRRVVLVEFPRKGIYSVGFLTSDQTGEVQEKTAEFVVN
                     VFVPTTPNPTSGFLIMVPKEGVIPLEMSVDDAIRMIMSGGIMMPEYAAQKTESGAAI"
     gene            4990..6555
                     /locus_tag="P5079_01700"
     CDS             4990..6555
                     /locus_tag="P5079_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY28732.1"
                     /translation="MALTTQDFELLLQANCILSSKLDSTGLLEAVMELATRVVKAETA
                     SILLLDEKTNELYFNVALGEAGEQIKQIRLKVGEGLAGWVAQNKQASIVNDVRNDPRW
                     SGKADEKSEFKTRQILAVPLLTKGKLIGVVEAINKVDDTPFSDQDRRAFEAFASQAAI
                     AIENARLFTEVKNEKEKIAAVFSQMSDGALLVDEAGNIQLMNVAAGHFFGVTPEDAIG
                     QMNVRHLTKDFMMIPSLETVYENKGSNCPVELRRKEGKAFYMDGVVNRLSGAKDEIAG
                     YLVVLHDVTEMRKEEMLKRNFLSLISHKLKTPLVTISGYAPLLLEDSSRLTEFQKKAL
                     TTIKNQGMHLSSLVDKLLNFSLVESETLSLDPKEVTVRALIEETVSNMKPYLDGHGAQ
                     MELDASLDAVSPVRVDVERMREVFRNLVENAVKFNSKSEKKVRLTASPQDKFVCVSVE
                     DNGPGIPSEEHPKIFQKFYQIEESFTGQVEGAGLGLALVKRIVEAHCGAVSVDSAAGR
                     GCIFHVTLPVSLR"
     gene            6585..7994
                     /locus_tag="P5079_01705"
     CDS             6585..7994
                     /locus_tag="P5079_01705"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRY28733.1"
                     /translation="MPFMPDPFARFLLRGDFADLYFEESTHRFLRWEDGRLDELVQGT
                     SSGVGFRCVRGEETLYGHLDGRAPFSGTWRSGEMRRLSELAGSLSAGLPARRRPMRFR
                     RLPVRRHRLRVPPDAVPLAKKIALLRRADRASRFSPELAQVTLSLGERRKRVLILNSL
                     GEWVEEERNYVVFVAQATAKKDGLLQTGHEVAGGLGGFELLDRALPERVARIAAGRAV
                     KKLRASPVVPGEMPVVISSQAGGTLIHEAVGHALEADSVQQGSSPHFAGRVGKKVAGA
                     KVTVLDDPTRAAGRGSFFYDDEASPSQRTVLIENGILRTYLYDRLTAKKEKRTSNGHG
                     RRESYAHRPIPRMSNTFVAPGKDSPEDIVRSLKRGLLVTRMGGGQVNTAGGDFVFEVE
                     EGFWVEDGRVLRMARGANLLGSAPEVLRSIDRVGSDMGWSVGTCGKDGQNVPVSDGLP
                     TLRVPKLLVGGTKSGKGRA"
     gene            7991..8821
                     /locus_tag="P5079_01710"
     CDS             7991..8821
                     /locus_tag="P5079_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY28734.1"
                     /translation="MKASFSKVSSFYFCPKKYEYRYVMELPVPTKAELVFGTALHAAL
                     EANFAQKIETRRDLPVEELLEVFRKELSEGLEKVPEESLRGPSDVHYLRGMGEHFLSR
                     FMAERAPALQPAPRGVECFFLLPLPGGHEISGKFDLMDTDWVLHDFKTSNKPYDRRRA
                     DKTQLVLYAWACERMFGRFPTALCFDVFVKGDGGEGAMDMQEPILFPVPSPGEMSQVA
                     QRLQRQIEKILEVQEKNAFTRAFVPLRCKWCEYQTVCTADWEKEGKPEPARVRMDYLV
                     "
     gene            complement(8822..10795)
                     /locus_tag="P5079_01715"
     CDS             complement(8822..10795)
                     /locus_tag="P5079_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY28735.1"
                     /translation="MSPLTPEWVPLLKKTFLFSSFSEDELAVVLEQMNRLSLPKGAVL
                     FREGDPGDALYLILSGRVRIARSANGEEKTIAYLGRSECLGEAAILTGEPRLSTAHVD
                     ATVELLVLYRKDFEPLLRSMPSMAIHIARVLSHRLLEADREQDAAYPSKIYPLIGTVP
                     RGDIVVFAVNLAVSLIQQTRRKVLLLDLTDQPEGVFIKSLGLTPVRVGESSLRQEDLQ
                     SPAVVQRLSVYHPSGLELMSLPLDLMEGKLYGSIYPFLSLLRQNYDITLLALPARTTP
                     LTRSIVDESDRLLLLEREAGRAGDELDEFRSSDKTLPVRLVKDSSVGPYDSVFQIRWD
                     DSLGESAFAQHTPFVLRDAQTHRQFDRLARKLGKMSVGLALGSGAAYGYALIGMLRVM
                     ERHGVYPDILSGTSIGALIGAFYAAGKSPDELEEIARGITKQKILSLADFTLPWQGVI
                     IGREVHKFLKSVIGDVTFEELALPLTCVATDIVSGEEVVLKTGKVADAVRGSLSLPFF
                     FQPYFLDGRFLVDGGLVNPVPSSAVAAMGADVLLSVNLTTRPGKKRMPGRRDWRRQSS
                     AYWKGPNLLEIVFKTLSTMQFEVAQARADISHAVFEPDLSNYTWMEFHRAEDIIKAGE
                     DYMEESILKIKTLLPFFADHCRMPLRPRPPAGY"
     gene            11017..11697
                     /locus_tag="P5079_01720"
     CDS             11017..11697
                     /locus_tag="P5079_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY28736.1"
                     /translation="MNIASFLKQIPLFHCLGEADLRRVARITKVREARKGETIFTKDT
                     WGDCLYIVVSGVVKIFSMSRTGKTKTFALLESRDFFGEMALLERGERSAGAVAATPAV
                     LLTIHRRDFQKLVKDRPQVAFVLLRTLCDRLRRADRDIEALSFNSVVGRVSGVLLDLA
                     KRYAKKGAREVRIDMDISHQELADMAGTAREMVTRVLNRFVRLGCLRMDGKRLTLLNV
                     QMLRDWIF"
     gene            11775..13199
                     /locus_tag="P5079_01725"
     CDS             11775..13199
                     /locus_tag="P5079_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28737.1"
                     /translation="MIRLEEFYAHPAVRERLAEYCGGRATAPDRFTAHYLVGYGSSLQ
                     SGGHGYPFESFSLKQFDDILDRGCDMFRSVWDHESTLGVLDLEYVNPRRPGEIFRHPR
                     AIFEKIEPIYRAVQKVLYRFNITPLAILTGQGYHFTFRVRQDSAAHRKLEGLGQVNGS
                     LGDKYRTPRGRRRAVVGEAIGRSFDGMGRLMEYVGHLVLRELRKQKVDSPVLTFTDVA
                     VGGAGESVSLDFSMYGDPLYMRDIRCPFSGYQKHKVQVWKVGEDVARQVPIQVALPRI
                     TGEGRSEWALEDILARRGHYERSAELASACRTDIPDVSHSVVNLIDSYTRSALYRFHL
                     AFDAEPQDPPERWPQTYDFFDSRSLPPCVAHCLATPNDHLLKPTNLQALVRVLMKRGW
                     HPKHIAGLVRSKYEKKLDWNMDWAKYDPATHANFFVRQFAGLLATGLDTEADLNCVSH
                     QEKGYCWKPFCGHNLADERLPAAG"
     gene            13206..14039
                     /locus_tag="P5079_01730"
     CDS             13206..14039
                     /locus_tag="P5079_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRY28738.1"
                     /translation="MDAGISTGIYYEQELVEHLPAIQKAGFRRLEVCVGGRPGLRPHF
                     DYNDASALDRLRQTLQTLGLSICSMHAPYSPGVDPSHPDAFVRKATLDEFCRAGRALK
                     SLGGSTLVVHVSVQEFNLSDRAEKERRLRNVRDVLSPLVEELRSQGIRLALENLLPHY
                     LGGDVAVLLDLVKPYPEEWAGVCFDTSHAHLWKTPSVYDMLDQALPRLAATHLSDNGG
                     RFDDHSLPGTGTMDWGRILKKLKKFQGTMLLEVFRDDKGSSPEDVLVRAFRWAQEFLN
                     G"
     gene            14056..15399
                     /gene="hisS"
                     /locus_tag="P5079_01735"
     CDS             14056..15399
                     /gene="hisS"
                     /locus_tag="P5079_01735"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRY28739.1"
                     /translation="MRYAAPRGTRDIKENEARRFYRLETVARDIFSRYNYEEIRTPVF
                     ETTDLFKRAVGETTDIVEKEIFEFEDRGGRKLALRPEGTAGIVRAYLEHSWDKTGGIR
                     KLFYVGPMFRAERPQAGRYREFWQIGAEYFGNPSAAADAEMLLLVRDFFQAAFKNHPG
                     AEKIVFTLNSIGCAKCRPDYRKALVNFLAGKKDKLCEDCQRRLEKNPLRALDCKVDGP
                     ALTDAPRMDQFLCEECRIHDAEMGTILNSFKFDFVRNPRLVRGLDYYNRTVFEVTIPG
                     IGAQDAVAAGGRYDSLVKTLGGPDVPAVGFALGMDRVAMALGEQAEEPSVKIYVAFTG
                     VQTSTMAIRLASAIRRPPRYSRLLFQAAKVELGQLNKSLKSQFRFADSWNADYVLIIG
                     EDEMSRGKVALKNLKAKTQVELDIAGSDDEAIARFVDKLYSENLSIPSESEKNSP"
     gene            15396..17234
                     /gene="aspS"
                     /locus_tag="P5079_01740"
     CDS             15396..17234
                     /gene="aspS"
                     /locus_tag="P5079_01740"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRY28740.1"
                     /translation="MKRTVYCGAVRKEHVGQKITLCGWVHNRRDHGGVVFVDLRDREG
                     LVQLVFHPDRPEVFKQGEGLRSEYVLEVQGEVRLRPAGTENPHLPTGAVEVWAETVSV
                     FNDCKTPPFEISEYSNASEDVRLRYRYLDLRRPPLQKNLLLRHRITQISRDYLVKNGF
                     LELETPFLGKSTPEGARDFLVPSRLSQGQFYALPQSPQLYKQLFMVAGFDRYYQIARC
                     FRDEDLRADRQPEFTQIDIEMSFIEEEDIITLIEGLVKAVFKEALGKDIPAPFPRLSY
                     DEAQSRFGSDKPDLRYAMEITDVSAVFQKTGFKVFSGVLAAGGVVRALCFKGGAAMSR
                     MEIDKLTEWVKGFGAKGLAWVKVTDKGPESSITKFFTSDELAGLQKAVAAAPGDIVFF
                     GADQAAVVSTYLGALRVELAKRAGLSKTQDVYKFCWVVDFPLFEYSEQDKKWNSVHHP
                     FTRPSPEAEEKLQDGSGDGVKRALGTFKSRAYDLVLNGTELGGGSLRIHRSRLQGKIF
                     EILNISPEAARAKFGFLLEALEFGAPPHGGIALGLDRFVALLAGEDSIRDVMAYPKTQ
                     KGTDPMSGAPSAVDELQLKEVGLKLSAAVVGKKKETSPPGETKPAA"
     gene            17376..17864
                     /locus_tag="P5079_01745"
     CDS             17376..17864
                     /locus_tag="P5079_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28741.1"
                     /translation="MTVEDEAASPVPLPEETRPAVDAHSLPGMRPAGVEVSFLSPPVS
                     RSAKYQRVWIWQETNDCLWNMAREHYGDPFLWPRIYEANRDLIRDPNVIFPKQQIVIP
                     PLETAQVYEPPMLPPPPPPVEEPVAVEPAPAPPPPPEPEKPAEPKADGELWKERRQFT
                     QW"
     gene            17858..18853
                     /locus_tag="P5079_01750"
     CDS             17858..18853
                     /locus_tag="P5079_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY28742.1"
                     /translation="MVTKRMALQDQEEAVFLFGPHDKNLKELERRFGVQVFVRPSSGV
                     GQEGLTLAVRGRSGSVDHALAALKEMKRRLPHSSAPPAPEAAVLLESNAFDEKTHPDA
                     IFMTITGRPIRPMTPQQKLYVEAIRTHDMVMAIGPAGTGKTYLAVASAMAAYKSGRVS
                     RIVLTRPVVEAGEKLGFLPGDFYSKVHPYLKPLYDAFYGMVGPEEFRLLRDEESIEII
                     PLAYMRGRTLDDAFIILDEAQNTTPEQMKMFLTRMGNGSRVIITGDITQIDLEDKSQS
                     GLVQISSILKHVEGIGFVWFTEGDIVRHGLVKKIIRAYNDWENRRKGGHGSPAKS"
     gene            18875..20407
                     /locus_tag="P5079_01755"
     CDS             18875..20407
                     /locus_tag="P5079_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRY28743.1"
                     /translation="MIGRYLSTPLRHVLLRILSWTEKHSETPRLQRVPLLKREVRIST
                     GWVALLVWAMLLVVLLFQFGLEWQNAVAVFLFTGMIMVLFIFYFRHDHPEILHDEEAV
                     MLLGVLAVGSVLMIQLWFQIVRSLPWVSPFGMPLAVTSILTVLLLQPRLAIILTTVLS
                     LLFGVIDNFSMTGTLVMFFGGLTGVARALYVRTRRDVTRAGLWVSLAQVLSILVLGFL
                     RHWPAQNIFFALLWGAVGGVLSSFLALSLLPYLESFFSRLTNIKLLELADVNHPLLKR
                     MSLEAPGTYHHSLIMASLAEPAAEAVGANGLLCRVGAYFHDIGKLVKPEYYVENQGAL
                     GNPHEPLPPNMSRLVIQSHVKEGMVLAQQYGLDKIIVDFVRMHHGTSRIEYFYRRAIE
                     QSADLDDLGTEEYRYPGPKPNSRETAILMLADSVEASVRTIDSPTHQRLQDQVGKIIG
                     TKMNDGQFDGVPLTLADLHEISESFVNTLTGIYHSRIPYPEASEEPELFPPPASNSLF
                     RT"
     gene            20404..20916
                     /gene="ybeY"
                     /locus_tag="P5079_01760"
     CDS             20404..20916
                     /gene="ybeY"
                     /locus_tag="P5079_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRY28744.1"
                     /translation="MSLPRRRRPSKKGRARSLRHIRFAREGGVRAPLPLRLARRAVSE
                     TLRSAGRREPLDVNVVWVSAPRIRALNRRFRSTDRGTDVISFRYDADKEFPSPAVSGD
                     LFVCVGRARYNARRFHVSVEEEAVRLLVHGTLHLLGFTDYVPVEKKKMWRRQEGIVRR
                     LGFGDAFPQI"
     gene            20922..21944
                     /locus_tag="P5079_01765"
     CDS             20922..21944
                     /locus_tag="P5079_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRY28745.1"
                     /translation="MSLIVLLFLFLAAAFISVAEAAIISLSTLRMKRLIVFHPALTAY
                     FSEWLAKPHRLIFTLMFLSNITLIAISSLSAAMVRHFDWGGIPLGVVEAVVWFVVTGL
                     LLVFGEIAPKIIGRALREKVAPWIVPIFGALTHYLTFLFAPLNWLIEKAAPRLQFSPA
                     GQLTVVSLEEFQHIIGESQVSGEVPFDASEMMRRVLALPQKTAADICQKKENVDVVAL
                     EVRKKHRGDELFVDLLVETGRSRVPVTRDGAFVGYVNVMDILQRWRSGEVTSVEELIR
                     QAPAVSASRRVVDLLEEFRRSGENLAFVFDDQKRFFGLVTLEDALEEIVGEILDEYDL
                     ELKKGD"
     gene            21952..22965
                     /locus_tag="P5079_01770"
     CDS             21952..22965
                     /locus_tag="P5079_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY28746.1"
                     /translation="MILFWGGLLMGVFFLTMSGFFSASETALMSLSKIQLGRLGKVYP
                     GRLTFWEKDADRVLAVILLANTLVNVALGVLATALAVQMEKVWGVPFRWGGWVVPVMV
                     GVLLIFVGEIVPKLVARAYTESLALKVAVPMRLITRALGPVVQGLVWMIGRALAVISR
                     SVRAEGGQWNVRVIRHLLDKTTLAPSVRSVLNNILDFGNLPVSRIMVPRDEIFAVDIA
                     LPKSEFVARVLSSGYSRVLVYKGNLDEIVGVVYTKDLLAFWRGESLIVVTDLIRAAFR
                     VTEGVPVAQLLREFRQGKYHMAVVLDRNRRVQGLVTLQDILETIVGDIAESPELGGSS
                     AVL"
     gene            23017..23712
                     /gene="recO"
                     /locus_tag="P5079_01775"
     CDS             23017..23712
                     /gene="recO"
                     /locus_tag="P5079_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY28747.1"
                     /translation="MQFNVNAIVLKSERVGESDKRLSIYTRERGRLWALAPGAARPRA
                     RLAPATEPCVEARFRLWAIPDNPFARITGGALTSAFPLLRADWDRMTSGIFLCEWTDR
                     LTPLEQTHAAKYELLRRSLAALEWSDDRPVVLAFLTQFLEQAGYVVGDEVLGAAHHAR
                     FRSLVADLRGYDFSTPFPELDEGVDGLEERLLRFVTPLLREPLKTLQHRKNLRMFMEK
                     THSPTAEPAPARN"
     gene            23726..24586
                     /locus_tag="P5079_01780"
     CDS             23726..24586
                     /locus_tag="P5079_01780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRY28748.1"
                     /translation="MTFQDIIITLHKFWAKQGCLLWQPYDLEKGAGTFNPATFLRCLG
                     PQPWAVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIIKPAPADIQNIYLSSLKAIGID
                     QRRHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQVGGIDLNPISVELTYG
                     LERLAMYSQKKNSVYDILWTDKMTYGQVHLEDERQFSKYNFESADVEMLRRHFGDWEN
                     EARRLLAEKLVLPAYDAVMKCSHLFNLLDARRAISVTERVGYIARVRALARGVAEAYV
                     AMSPSEEGQK"
     gene            24583..>26606
                     /gene="glyS"
                     /locus_tag="P5079_01785"
     CDS             24583..>26606
                     /gene="glyS"
                     /locus_tag="P5079_01785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRY28749.1"
                     /translation="MKRPSFIAMREPEPRDFLLEIGVEELPARFVPPALDNLRQLAAA
                     QLETAGLSYAADGLQTWGTPRRLTLFIRALAGKSKDQVQENMGPAVAQAKDADGHWTP
                     AAQGFARSQGVSLEKLEVRETEKGPRLCAVRRTLGVPTEKILPEMLSAVLGKLNFPKN
                     MVWEKSRFSFARPLRWLVALYGSQPVKFEIAGVKAGRKTYGLRVQGGKSFDVVSPAKY
                     GMLLKDRCIVVDPSERRHLIEKQIHQAVKDAHGKVRLDQDGALLDEVVNLVEHPVAIL
                     GKFDEKYLALPPEVLVTSMKKHQKFFPVYAADGRRLTSRFVGIRNGVSENQAVVREGY
                     ERVLAARLADAAFFYSEDKKKKLEDNLSLLQGITFLAPLNLLNKTTRVEKLIDGLLPS
                     LPMGDDRKECARRIARLAKTDLLTAMVGEFPELQGVMGRIYASEHENPEVARGIEQHY
                     WPLAAEGDLPDSDSAAVVSVADKLDSLAGNYLVGKVPSGSQDPYGLRRAAVGVLRIVS
                     DRRWRMNLSDIALAAMREYPFWNEKVGPPAHAQLVSFLRQRWAALMAAAGFRFDEVEA
                     AASAGFGDVVEVFARLKALRDIRSHAHFASLSVAFKRADNILKQAEKQAVKGYHIPRP
                     MDGRSILIREELLRDGSERALFSAVVQCEEANAPLLEAGDYAAAYGNLVAL"
BASE COUNT         4915 a         7524 c         8677 g         5490 t
ORIGIN      
        1 ctggtggacg tcggccaccg ccgtgaccag ctcctcgtgc gcgcgcttgc gcgttgatcg
       61 agagcgcgct gggggcgttg gaaccttttg gcgccgcggc ggctccgttg cggctcatgg
      121 cggagtttgt tcgtgcccgc gcccgctgac gctcttcgca ccaccttgcg cgccgccctg
      181 attctggcgg cgttcgtggc cattgtggcg gccaactcgc tggtgaagat cggcgggctc
      241 tcgctgaccc ccctgctgac ggcgacgcgg agtcccgacg tcaacgtgcg cgtggtgtcc
      301 tgcgtggcgc tgggggactt gggcgatccg tcggccgtgc cggcgctgga acgctgcgcc
      361 gccgatgagg ccggggacgt ccgctcggcc gcgcagaccg ctttgaataa gatccgctcg
      421 tcgacctccg cttcccgcta aaactcgata ttcggttgaa aaaggaactg gaagaccgtg
      481 cggcgcgcgt cgccgaggtc gccggtgtat ttcttctcgg accgccagag gaatcccagc
      541 gccagcgacg gcacgacctt tttgtccggc aaggggaact tcttgtccac cacgaagtgg
      601 tgctggacgg ccgataagcg ggactgatcg aaatcgaatt tatacatgat gccgctgttg
      661 ttcagccagg agaaccctct ttccttgaaa tggatgcggc tccgccggac gcccaggtaa
      721 tagacgtcgg tgatctcctt gcgcgcgtgg aatttggcgc cgatgcggaa actccagcgc
      781 agttccatgt ccggatagat ccggtcccct ttcaccttcc attccgtttc cacccagtca
      841 tccttcatca tgtcgttgtc tttgatgtga taattccccg cgctgacgag gtagccgatg
      901 tgcccgcgtc cctcggcgta atctcgtttc ttgacgggct tgaaatccac gatgttcccg
      961 aaaaacaggg agaccgcggc gggctcctcg aagccggtgg tgatgctgcg gatgaggttg
     1021 ctgtcttcgg agaactgggt gtcgtcgtag aagtctcgcg cctgccgttt gagatagacc
     1081 ccaaggagag ggagggggtt cacgctcgtt tccaagagga ggaagcgggg gatgggggag
     1141 gagaggatga gttttttgta gatgtccgtt tcgcttttga attcgtgata ggggatgggg
     1201 gattgggtga ggttgacgta atagtccacc gaggtgtaat acgcgtccag ttggtattcg
     1261 atctcattgg ccgcgccgaa aaccgagaaa aggaggggca cgccgccggc gagaagggcg
     1321 cgtttgagtg ttttcatgag ggaacggcgc tgtcccgcag agtcgtttta tctttcagca
     1381 cgggggaaag tttacaaaaa gacgcggagc ggacgcgaga gggggcggtt ttaggaagag
     1441 agggccggca gggaaaaaat gaaggtggcg cctttgccgg gcgcgctttc cacccagatt
     1501 cggccgccgt ggacctgcac gagtttcttg caaatggcca ggccgaggcc ggtgcttttt
     1561 tcgtcgtgca ggggttgggc gctcccccgc ccgaactccg tgaaaagaga agggatttcc
     1621 tttgcgggga tcccgatccc ctggtcggcg acggaaatcc acgcctcgtt ttcacggttc
     1681 tccgcccgca gcgaaatggt ggtgcggaac ggagaatatt taatggcgtt ggacaggagg
     1741 ttctccagga cctggccgat gcggttggcg tccatccgca ccttgggaag gtccggatcc
     1801 agggtcagag tgaactcgaa atcggcggct tgggtgatgg ggagatgcgt catgtgaaaa
     1861 tccttcagga attcggcggg atcggtttcc tggaggacga gggccagttg tccctgctcg
     1921 atggagtgga tgtccaggag gtcgttgatc aggtagatca tggaattgca cgatttcttc
     1981 gccagccgca tcagttgcga aagagtttcg gtccgggtca cgccgcggcc gtcggtggca
     2041 taggaaaggt acccccagat gaccgagagc ggttgtcgca ggtcgtgcac caccatcccc
     2101 aggatgcggc tcctttgttc gttcagcgcg agcagggaat cgtgcaggcg gcctttttcg
     2161 atgatttcgg agatcagtcc ggcgatcaga agaaaaatgt cggcatgggg attccggtag
     2221 gtgtccctct gccggctgga gaaaaaaaga aacccgatcg ctttcccctc ggcgatgaga
     2281 gggcaggtga ggctggaacg gacgccttcc ttcaggatga gccgggtgga ctccgattcg
     2341 ggatgggaac tcagatagga ttccaggtcg ttgatgatcc gcggtcggcc ggtctcggcg
     2401 actttttgca gactgctgtt tttgaggggc gcgctgtaat cgggagggaa gaattggggg
     2461 gaggtttccg agcgccccca gcgcgtccgg acggtggcgc cggcctcctc gatgagcgcc
     2521 accccgatgc ggtcgtaggg gatgacggcg cggaaggaat catagaccag gttgaggatc
     2581 tcctccaggg aacggccgga actgatcttt tcggtgatct tgaggagggt gtggatttcc
     2641 ccgagttttt tttccatggc cgggcccaaa tccaccagcg cccttccgag ccggccgatg
     2701 tcgtcgtcga aaccgcaagg gagctcgatt tggaaggaac catcttttaa cgtttctacg
     2761 gccttgagat attccggaat tcgagggtcg ctcaatgcaa cctcccggcg cggggtaaac
     2821 cgtccaggcc actttcccag aaaaagggaa cccccgccta tacatgacta tttatgtcat
     2881 atttagtata tccctctttt caccctttgt caagaacggg accgtcgaga gaacatactt
     2941 ttcgattttt tgggggcacc ggcgggtgga cgacgggggc tgcccgacga aggaggggta
     3001 tctgctacaa taggagaagg gcgtttcgac gggcccggta agggccgggc cggcggccga
     3061 gcggcaaaaa agggaatgcc gtgcgtcggc cttggccggg aagagcgggg ggccaaggaa
     3121 cgggaacgaa gttcccgtgg accggccgaa accccgtggt ttgagccccg cctgggagcg
     3181 ataaaaaaag gaatgccgga ggcgggactt gaacccgcac gcagcctaag ctgctcgaga
     3241 ttttgagtct cgcgcgtctg ccaatttcgc caccccggcc acgcggcaca ttatctcttt
     3301 ttggcgggac aacgtcaatt cggcgggccg acggcgcggt tcggaggatc gcatgaggat
     3361 cggagtccat tgttcggtgc ggaacggttt cgtgggcgcg ttgcgtgagg cggcggcgct
     3421 gggctgcgaa acactgcaga tctttacgca aagcccccgc ggctggcgga cgcgcgtcta
     3481 tgaaccgacg gaattctcgg atttccgacg ggagcgggag cgcctgggac tggggcccgt
     3541 ggtggtgcac tcgccctatc tgcccaacct gtgcacctcc gacgcggcgc tctacgcgcg
     3601 ctccgtccgg gccctgcggg aagatttgga tcgttgcgaa aagctggggg cggaatatct
     3661 ggtgctccat ccgggggcct actccccctc ggccgattac aaccggggcg tggaacgtct
     3721 ttcctcggcg ctcgacgcgg ttttgggcgc ggcgggggaa gggacgcgcc tcctcgtcga
     3781 gaacatggcc gggggcgggc ggcggatcgg gagttcgttc cgggaaatcg ccgacatttt
     3841 ttcgaacgtg aggcatcgtt cccggttggg ggtctgtttc gatacctgcc acgctttggc
     3901 cgccgggtat gatctgacat cggcgtccgg catcgagaag accttcgagg aattcgatcg
     3961 ggcgctggga ctgaaacaga tccgggcgtt ccacgtgaac gattccaaga ccctgcgggg
     4021 cagtcgccgg gacatacacc aacacatcgg gcggggaatc atcggggtcg aggggttccg
     4081 ccggatattt tcgcggcgga tcttcttcgg actgccgttg attctggaaa cccccaagga
     4141 ttctccccgg gccgaccgtc gcaacctccg tgtcctccgg cgctgtcttc ccgaatgaag
     4201 gcggacgggc ggcggggcgc gcagtttccc cgatttttga acgcaacagg gggttttttt
     4261 ggtatactgc catctcacat tgaaaactac cttctgagac ggtaaaaaca gtgtccaaaa
     4321 agagccattt taagggatat ttcctcaccg gtttggtcgc gctcctgccg ctgtgggtga
     4381 cgatcattct gttgcgggcg ctgttctata tcatttccaa cgccacccgg cccatcctgc
     4441 agccgatcct tcgcgaatgg accccgctcg aatacgcccc cttcttgttc gacctgacct
     4501 gtttcctcgg caccttgctc atcgtttatc tggtgggggt gttggtgtcg aacctggtgg
     4561 gccggcagat cttcgtcaag gtggaaagac tcattttccg tctgcccatc gtggcggacg
     4621 tctatcaggc cgtgcgcaag ttgacggata tgttctccag caacgccaat agtaaatttc
     4681 gacgcgtggt gttggtggag ttcccgcgga aagggattta ttcggtcggt ttcctcacca
     4741 gcgaccagac gggcgaggtg caggaaaaga cggccgaatt cgttgtgaac gtttttgttc
     4801 cgaccacgcc caaccccacg tccggttttt tgatcatggt gccgaaagag ggcgtcatcc
     4861 cgttggagat gtccgtggac gacgccatcc ggatgatcat gtccggaggc attatgatgc
     4921 cggaatacgc cgcacagaag acggaatcgg gggcggcgat atgacgcacg gaccggcgcg
     4981 aggcgaacga tggcgctgac gacgcaggat ttcgaactcc ttctgcaggc caactgcatt
     5041 ctttcctcca aactggacag caccggcctc ctggaggcgg tgatggaact ggccacgcgg
     5101 gtggtcaagg ccgagacggc ctccatcctc cttctggacg aaaaaacgaa cgaactttat
     5161 ttcaacgtcg ccctgggcga ggccggggaa cagatcaaac agatacggct caaggtgggg
     5221 gaggggctgg ccggctgggt ggcacagaac aagcaggcct ccatcgtcaa cgacgtgcgc
     5281 aacgatcccc gctggagcgg caaggcggac gagaagagcg aattcaagac ccgccagata
     5341 ctcgccgtgc ctctcctgac caaggggaag ttgatcggcg tggtggaggc catcaacaag
     5401 gtggacgaca cccctttttc cgatcaggac cggcgggcct tcgaggcgtt cgcgtcgcag
     5461 gcggccatcg ccatcgagaa cgcccgtctc ttcaccgagg tgaagaacga gaaggagaag
     5521 atcgcggcgg ttttctcgca gatgtcggac ggcgccctcc tggtggacga ggccggcaac
     5581 atccagctca tgaacgtggc ggcggggcac tttttcggag tgactccgga agacgccatc
     5641 ggccaaatga acgtgaggca tttgaccaag gacttcatga tgatcccgtc cttggagacc
     5701 gtctatgaaa acaagggatc gaactgcccc gtggagctcc ggcgcaagga agggaaggct
     5761 ttttatatgg acggggtggt caatcgtttg tcgggcgcca aggacgagat cgccggctat
     5821 ctcgtcgtcc tgcacgatgt gaccgagatg cggaaagagg agatgctcaa aaggaatttc
     5881 ctctcgttga tttcccataa actcaagacc ccgctggtga ccatttcggg atacgcgccg
     5941 ctgctcctgg aggattcctc ccggctcacc gaatttcaga agaaggccct caccaccatc
     6001 aagaaccagg ggatgcactt gtccagcctg gtggacaagc tgctcaactt cagcctggtg
     6061 gaatcggaga cgctgtcgct ggaccccaaa gaggtcacgg tccgggcgtt gatcgaggag
     6121 accgtctcca acatgaagcc ctatctggac gggcacggcg cccaaatgga gctggacgcc
     6181 tctttggacg cggtatcgcc ggtgcgggtg gatgttgaac gcatgaggga ggtgttccgt
     6241 aatctggtgg agaacgccgt caagttcaac tccaaatcgg agaagaaggt ccgactcacc
     6301 gcttcgcctc aggacaagtt cgtctgcgtg tccgtggagg ataacgggcc ggggatcccg
     6361 tcggaagagc accccaagat tttccagaag ttctatcaga tcgaggaatc tttcaccggc
     6421 caggtggaag gggcggggtt gggtctcgcc ctggtgaaga ggatcgtgga ggcccattgc
     6481 ggcgccgtca gcgtggattc cgccgcgggc cggggctgca ttttccacgt gacccttccg
     6541 gtttccttga gataatccct ttctcctcct ttcaaaacaa ccccatgcct ttcatgcccg
     6601 acccgttcgc ccggttcctc cttcggggcg acttcgccga cctctatttt gaggagtcca
     6661 cccatcgttt tctccgttgg gaggacgggc gactggacga gctcgtccag ggaacgtctt
     6721 ccggtgtggg gttccgctgt gttcgggggg aggagaccct ctacggccac ctggacggcc
     6781 gcgcgccgtt ttccgggaca tggagatccg gcgagatgag gcgtctttcc gaactggcgg
     6841 gttcgctctc cgccgggttg ccggcgcgcc gtcggccgat gcggttccgg cggcttccgg
     6901 tccgccggca ccgcctgcgg gtcccgcccg atgccgtccc gttggcgaaa aagatcgctt
     6961 tgctgcggcg ggcggaccgc gcctcccggt tctcgcccga gttggcgcag gtgacgctgt
     7021 cgttagggga gcggcggaag cgcgtgctca tcctgaattc gttgggggaa tgggtggagg
     7081 aggagcggaa ttatgtcgtg ttcgtggcgc aggccacggc gaaaaaggat ggactgttgc
     7141 agacgggcca cgaagtggcc ggagggttgg gcgggttcga attgttggac agggctctcc
     7201 ccgaacgcgt ggcccggatc gcggcggggc gggcggtgaa aaaactgcgg gcgtcgcccg
     7261 tggtgccggg cgagatgccg gtggtcattt cgtcccaggc cggcggcacc ctcatccacg
     7321 aagcggtggg gcatgcgctg gaagcggaca gcgttcagca gggatcttcg ccgcatttcg
     7381 ccggccgggt ggggaagaaa gtggccggcg ccaaagtgac ggtgctcgac gaccccaccc
     7441 gcgccgccgg ccggggatcg tttttttacg acgacgaggc ttctccctct cagcgcacgg
     7501 tcctgatcga gaacgggatc ttgcggacct atctttacga ccggctcacg gcaaaaaaag
     7561 aaaaacggac ttccaacggg cacggccgcc gcgaatccta cgcccatcgg cccatcccgc
     7621 gcatgtccaa cacgttcgtg gcgcccggaa aagattcccc ggaggacatc gtccgttctc
     7681 tgaaacgcgg actcctggtg acgcgcatgg gcggcggcca ggtgaacacc gccggcggcg
     7741 atttcgtttt cgaggtggag gagggttttt gggtcgagga cggacgggtc ctccggatgg
     7801 cgcgcggggc caacctgttg ggcagcgcgc cggaggtgtt gcgttccatc gaccgggtcg
     7861 gttcggacat gggatggtcc gtcggcacct gcggcaagga cgggcagaac gtccccgttt
     7921 ccgacgggct gcccacgctg cgagtgccga aactcctggt gggcggcacg aaaagcggaa
     7981 aggggcgggc atgaaagcaa gtttttccaa ggtcagttcg ttctatttct gcccgaaaaa
     8041 atacgaatac cgctacgtga tggaactgcc ggtgcccacc aaggcggaac tggtcttcgg
     8101 cacggccctg cacgcggccc tggaggcgaa tttcgcccag aagatcgaga cccgccggga
     8161 cctgccggtg gaggaactgc tggaggtttt tcggaaggaa ctgtcggagg gtttggaaaa
     8221 agtgccggag gagagcctgc gcggcccgtc ggacgtccat tatttgcggg ggatggggga
     8281 acatttcctc agccgtttca tggccgagcg ggcgccggcg cttcagccgg cgccgcgggg
     8341 agtggagtgt tttttccttc tgcccctgcc cggcggccac gagatcagcg ggaagttcga
     8401 cttgatggac accgactggg tgctgcacga tttcaagacg tccaacaagc cctacgaccg
     8461 ccggcgggcc gataagacgc agctggtgct gtacgcttgg gcttgcgagc ggatgttcgg
     8521 ccggttcccg acggcgcttt gtttcgacgt tttcgtgaag ggagacgggg gggagggggc
     8581 gatggatatg caggagccca tcctgtttcc cgtgccgtcc ccgggggaga tgtcccaagt
     8641 ggcccaaaga ctgcagaggc agatcgaaaa gatcctcgaa gttcaggaga agaacgcttt
     8701 cacgcgggcg ttcgtgccgt tgcgctgcaa atggtgcgaa tatcagacgg tgtgcacggc
     8761 ggactgggaa aaagagggga agcccgaacc cgcgcgggta aggatggatt atctggtgtg
     8821 atcagtatcc ggccgggggc cggggtcgga gcggcatgcg gcagtgatcg gcgaaaaagg
     8881 gcagcagggt cttgatcttc aggatgcttt cttccatgta gtcttctccc gccttgatga
     8941 tgtcctcggc tcgatgaaat tccatccagg tgtaattcga caggtccggt tcgaacacgg
     9001 cgtgtgaaat gtcggcgcgc gcctgcgcca cctcgaactg catcgtcgag agcgtcttga
     9061 agacgatctc cagtaaattg gggcctttcc aataggccga ggactgccga cgccagtcgc
     9121 gccggccggg catgcgtttc ttgcccggcc gcgtcgtcag gttgacggac aacaggacgt
     9181 ccgcccccat ggcggccacc gccgacgacg ggaccgggtt caccaggccg ccgtccacca
     9241 ggaaccggcc gtccaggaag tagggctgga agaagaacgg caacgacaac gacccgcgca
     9301 cggcatcggc cactttgccg gtcttcaaca ccacctcttc gccggagacg atgtccgtgg
     9361 ccacgcaggt gagcggcagg gcgagctctt cgaacgtgac gtcgccgatg accgacttga
     9421 ggaacttgtg cacctcccgg ccgatgatga cgccttgcca ggggagcgtg aaatccgcca
     9481 gcgagagaat cttctgtttg gtgatgccgc gggcgatctc ctccagttcg tcgggcgatt
     9541 tcccggcggc gtagaacgcc ccgatcagcg cgccgatgga cgtgccggag aggatgtccg
     9601 gatagacccc gtggcgttcc atcacccgca acatgccgat cagggcatag ccgtaggccg
     9661 cccccgatcc cagggccagc cccacgctca tctttcccag ttttcgcgcc aggcggtcga
     9721 actgacggtg ggtctgggcg tcccgcagga cgaacggcgt gtgttgcgcg aaggccgact
     9781 ctccgagcga gtcgtcccag cgaatctgga aaacggagtc ataggggccc accgaggaat
     9841 ctttcaccag ccggacgggg agcgtcttgt cggaggaacg gaactcgtcg agttcgtcgc
     9901 cggcgcgccc ggcttcgcgc tccaggagca gcaaccggtc ggattcgtcc acgatggacc
     9961 gcgtgagcgg cgtggtgcgc gcgggcaggg ccagcagggt gatgtcgtag ttctgcctca
    10021 gcagggagag gaacggatag atcgacccgt agagtttgcc ctccatgagg tccaggggaa
    10081 ggctcatgag ttccaacccc gacgggtgat acaccgacag ccgctgcacc accgccggag
    10141 attgcaggtc ttcctgccgc agggaactct cccccacgcg caccggcgtc aggccgagcg
    10201 atttgatgaa gacgccctcg ggttggtcgg tcaggtccaa caggaggact ttgcggcggg
    10261 tttgctggat gagggagacc gccaggttta ccgcgaagac cacgatgtcg ccccgaggca
    10321 cggtgccgat gaggggatag atcttcgacg gataggcggc gtcctgttcg cggtcggctt
    10381 ccaggaggcg gtgcgagagg acccgggcga tgtggatggc catggaaggc atggaacgga
    10441 ggagcggttc gaaatctttc cggtagagca ccaggagttc cacggtggcg tccacgtggg
    10501 cggtgctgag gcgcggttcg ccggtgagga tggcggcctc tcccaggcat tcgctccggc
    10561 cgaggtaggc gatggttttt tcctcgccgt tggccgaccg ggcgatgcgg acacggccgg
    10621 agaggatcag gtagagcgcg tcgccggggt ccccttcacg gaacaacacc gcccccttcg
    10681 ggagggagag ccggttcatc tgttccagaa ccacagccaa ttcgtcttcg ctgaacgaag
    10741 aaaaaaggaa ggttttttta agaagcggga cccattcggg ggtaagggga gacattcttt
    10801 tttgggttga ttttctgtcg attttccgat aacgataacg aaataaaatt gctttgtcaa
    10861 atggggcgga aaaatgataa actgccgtcg ttggaaccca cgcgcataac aaattcagat
    10921 tgtcttttat aacaaaatca aacgtcaagg aagtgtcgtg aggggtcgtt gcccggcttt
    10981 tggcgtcgga attgtcgctt aacggaggag attcccatga acatcgccag ctttcttaag
    11041 caaatcccgc ttttccactg tctgggggag gccgatcttc ggcgggtggc ccgcatcacc
    11101 aaagtgcgtg aggcccgcaa gggcgagacg attttcacaa aagacacgtg gggtgactgc
    11161 ctttacatcg tggtcagcgg cgtggtgaag attttcagca tgtcccgcac gggcaagacc
    11221 aagaccttcg cgttgctgga atcgcgcgac tttttcggcg agatggcttt gttggagcgg
    11281 ggggaacgtt cggccggggc ggtggccgcc accccggcgg ttttgttgac gatccatcgg
    11341 cgcgattttc agaaactggt gaaagaccgg ccccaggtgg ctttcgtttt gctgcgcacc
    11401 ctctgcgacc gccttcggcg cgccgaccgg gacatcgagg cgctctcttt caacagcgtg
    11461 gtcggccggg tctccggggt gctgctcgat ttggccaagc ggtacgccaa aaaaggggcg
    11521 cgggaggtcc gcatcgatat ggacatctct caccaggaac tggccgacat ggccggcacc
    11581 gcccgggaga tggtgacccg cgtcctcaac cgcttcgtcc gtttgggatg ccttcggatg
    11641 gacggaaaac gactgaccct tttgaacgtc cagatgcttc gcgactggat tttttgaccg
    11701 ttccgccatc cgtgtccttc ctctgagagc gtgctgactc gaaccgcccc atccgacatt
    11761 tcggcgattt ccgcatgatc cgtctggaag agttctacgc ccacccggcg gtgcgggaac
    11821 ggttggccga atattgcggc ggccgcgcca cggcccccga caggttcacg gcccattatc
    11881 tggtgggata cggctcctcc cttcagtccg gcggccacgg ctatccgttc gagtctttct
    11941 ccctgaaaca gttcgacgac attctggacc gcgggtgcga catgttccgg tcggtgtggg
    12001 accatgagtc caccttgggc gtgctggacc tggaatacgt caatcctcgc cggccggggg
    12061 agattttccg tcacccgcgc gcgatctttg aaaagataga gcccatctac cgggcggtgc
    12121 aaaaggtgtt gtaccggttc aacatcacgc cgctggccat cctgaccggc caggggtatc
    12181 acttcacgtt ccgggtccgg caggattcgg ccgcccaccg gaaattggag gggttggggc
    12241 aggtgaacgg ttctttgggc gataaatacc gcaccccgcg cggccgccgc cgggcggtgg
    12301 tcggcgaggc catcggccgt tcgttcgacg gcatgggacg gctgatggaa tacgtcgggc
    12361 acctggtgtt gcgggaactg cgaaagcaaa aggtcgattc cccggttttg acctttacgg
    12421 acgtggccgt cggaggggcg ggggaatcgg tgtccctcga tttctccatg tacggcgacc
    12481 ctctctacat gcgggacatc cgctgccctt tttcggggta tcagaaacac aaggtgcagg
    12541 tctggaaggt gggggaagac gtggcgcgcc aggtgcccat ccaggtggct ctgccgcgca
    12601 ttacgggcga ggggcggtcg gagtgggcgc tggaggacat cctcgcgcgc cgggggcatt
    12661 acgaacgctc ggccgaactg gcttccgcct gccgcaccga catccccgac gtctcccatt
    12721 ccgtcgtgaa tttgatagac tcctacacgc ggtcggccct ttatcggttc catctggctt
    12781 ttgacgcgga accccaggat ccgccggaac gctggcccca gacatacgat tttttcgatt
    12841 cccggtccct ccccccgtgc gtggcgcatt gccttgcgac gcccaacgac catctgctca
    12901 agcccaccaa cttgcaggcc ttggtgcgcg tgctgatgaa gaggggatgg catccgaaac
    12961 acatcgccgg gctggtccgg tcgaaatacg aaaagaaact cgactggaac atggactggg
    13021 cgaaatacga cccggccact cacgcgaatt tttttgtccg gcagttcgcc ggacttctgg
    13081 ccacggggtt ggacacggaa gcggacttga actgcgtttc tcatcaggaa aagggctatt
    13141 gctggaagcc cttctgcggc cacaacctgg ccgacgagcg tctgccggcg gcgggctgaa
    13201 aaagaatgga cgccgggatc tccacgggga tttattacga gcaggagttg gtcgaacacc
    13261 tgccggcgat acagaaggcg gggttccgcc ggttggaggt gtgcgtgggc ggccggccgg
    13321 gattgaggcc gcacttcgat tacaacgacg cctccgccct ggaccggctc cggcaaacgc
    13381 tgcagaccct gggtctttcg atttgctcca tgcacgcgcc ctattcgccc ggcgtggatc
    13441 cgtctcaccc ggacgccttc gtgcgcaagg ccaccctgga cgagttctgc cgggcgggcc
    13501 gcgcgctgaa atcgttgggc ggctcgacgc tcgtggtgca cgtgtcggtg caggaattta
    13561 atttgtccga ccgcgccgag aaggagcgcc gcctgcggaa cgtgcgcgac gtgctgtctc
    13621 cgctggtgga ggagttgcgt tcgcagggga tccgcctggc gctggaaaat ctactgccgc
    13681 actatctcgg cggcgacgtc gccgttttgt tggatttggt caagccgtat ccggaggaat
    13741 gggcgggcgt gtgtttcgac acctcccacg cgcacctgtg gaagacgccc agcgtctacg
    13801 acatgctgga ccaggccctg ccgcgcttgg cggccacgca tctttccgac aacggcggcc
    13861 gcttcgacga ccactccctg ccggggacgg ggaccatgga ctggggacga attttgaaaa
    13921 agctgaaaaa attccagggg acgatgctcc tggaggtttt tcgggacgac aagggctctt
    13981 cgccggagga cgtgcttgtc cgggcgttcc gatgggcgca ggaatttttg aacggataga
    14041 cggaggagaa agaacatgcg gtacgcggca ccgcgcggca cgcgcgacat aaaggagaac
    14101 gaggcccgcc gtttttaccg tctcgaaacg gtggcccgcg atatcttttc gcgttacaat
    14161 tacgaggaaa tccgcacccc cgtttttgaa accacggacc tgttcaagcg cgccgtcggc
    14221 gaaaccacgg acatcgtgga aaaggaaatc ttcgagtttg aggacagagg gggccggaaa
    14281 ctggcccttc ggcccgaggg gaccgcgggg atcgtgcggg cctatctgga acacagttgg
    14341 gacaagaccg gcggtatccg taaattgttc tatgtgggcc ccatgttccg cgcggaacgg
    14401 cctcaggccg gacgctaccg tgagttttgg cagatcggcg cggaatattt cggaaacccg
    14461 tccgccgcgg ccgatgccga gatgcttttg ctggtgcgcg attttttcca ggcggcgttc
    14521 aagaaccatc ccggcgccga aaaaatcgtt ttcacgttga actccatcgg ttgtgccaaa
    14581 tgccggccgg attatcggaa ggcgttggtg aattttttgg caggcaaaaa agacaaatta
    14641 tgtgaggatt gccagaggcg gctggagaag aatccgcttc gggctttgga ctgcaaggtg
    14701 gacggccccg ctctgacgga tgccccgcgc atggatcagt tcctctgcga ggagtgccgg
    14761 atccacgacg ccgaaatggg gacgattttg aacagcttta aattcgattt cgtccggaac
    14821 ccgcgactgg tgcgcggttt ggattactac aaccgcaccg ttttcgaggt gacgatcccc
    14881 ggcatcggcg cacaggatgc ggtggcggcc ggcggccggt atgattcgct ggttaaaacg
    14941 ctgggcggtc cggatgtccc ggccgttggt ttcgccctcg gcatggatcg ggtggccatg
    15001 gcgttggggg aacaggcgga ggaaccctcg gtgaagattt atgtggcgtt taccggggtt
    15061 cagacatcga cgatggccat tcgactcgct tccgccatac ggcggcctcc tcgatattcc
    15121 cgcctgttgt ttcaggcagc caaggtcgag ctcggacagt tgaacaagag tttgaagtct
    15181 caattccggt tcgccgattc gtggaacgcg gactatgttt tgatcatcgg cgaggacgaa
    15241 atgtcccggg gcaaggtggc gctcaaaaat ttaaaagcca aaacccaggt tgaattggac
    15301 atcgccgggt ccgacgatga agccatcgcc cgatttgtgg ataagttgta ttcggaaaat
    15361 ttgtcaatac cttcggaaag cgagaagaat tctccatgaa acgcacggtt tattgcgggg
    15421 cggtacgaaa agaacacgtc gggcagaaga tcaccttgtg cggctgggtg cacaaccgcc
    15481 gcgaccacgg cggcgtggtg ttcgtggacc tgcgcgaccg cgagggactc gttcagcttg
    15541 tttttcaccc cgaccgcccg gaagtattca agcagggcga aggccttcgc tccgaatacg
    15601 tgctggaggt gcaaggcgag gtgcgcctcc gtcccgccgg cacggagaac ccccacctgc
    15661 ccacgggcgc ggtggaggtg tgggcagaga cggtgagcgt gttcaacgac tgcaagacgc
    15721 cgccgttcga gatctccgaa tattccaacg ccagcgagga cgtgcggctg cgctaccgat
    15781 atctggacct gcgccggccc cctctgcaaa agaatctttt gttgaggcac cgcatcaccc
    15841 agatatcgcg cgactatctg gtgaaaaacg gatttctgga gctggagacg ccctttctcg
    15901 gaaaatccac gccggaaggc gcccgcgatt tcctcgtgcc gtcccgcctc tcgcaggggc
    15961 agttctatgc cttgcctcaa tcgccccagc tttacaaaca gctttttatg gtggccgggt
    16021 tcgaccgcta ttatcagatc gcccgctgtt tccgcgacga ggacctgcgc gccgaccgcc
    16081 agccggagtt cacccagatc gacatcgaga tgtcgttcat cgaggaagag gacatcatca
    16141 cgctcatcga gggattggtg aaggcggttt tcaaggaagc cctggggaaa gacattcctg
    16201 cgccgttccc gcgcctttca tacgacgaag cccagagccg tttcggttcg gacaagcccg
    16261 atctccgcta cgccatggag atcacggacg tgtcggctgt gttccagaag acgggtttca
    16321 aggttttctc cggcgtgctg gcggccggcg gggtggtgcg cgcgttgtgt ttcaagggcg
    16381 gcgcggccat gtcgcgcatg gagatcgaca agttgacgga gtgggtcaaa gggttcggcg
    16441 ccaaggggct ggcctgggtg aaggtgaccg acaaggggcc ggagtcctcc attacgaaat
    16501 ttttcacgtc ggacgaactc gccggcctgc agaaggccgt ggcggccgcg cccggggaca
    16561 tcgtgttctt cggcgcggat caggccgccg tggtgtcgac gtatctggga gcccttcggg
    16621 tggaactggc gaaacgggcg ggcctgagca aaacgcagga cgtctataag ttctgctggg
    16681 tggtggactt tccgcttttt gaatacagcg agcaggataa aaagtggaac agcgtgcacc
    16741 atccgtttac ccggccttcg ccggaggcgg aggagaagct tcaggatggt tccggggacg
    16801 gcgtcaaacg ggctctcggg acgttcaaat cccgcgccta cgatctggtg ttgaacggca
    16861 ccgaactcgg cggcggcagc ttgcgtatcc accgcagccg tctgcagggg aagatcttcg
    16921 agattttgaa catctctccg gaagccgccc gcgccaagtt cgggttcctt ctggaagcgc
    16981 tggagtttgg cgcgccgccg cacggcggca tcgccctggg actggaccgg tttgtggccc
    17041 tgctggcggg agaggattcc atccgcgacg tgatggccta ccccaagacg cagaagggga
    17101 cggaccccat gagcggcgcg ccgtcggcgg tcgacgagtt gcagttgaaa gaagtggggc
    17161 tgaaactttc ggcggcggtc gtcgggaaga aaaaagagac ttcgccgccg ggcgagacaa
    17221 aaccggcggc ttgacgggcg tttgtgggga atgaacggaa accgatgatt ttttcgacga
    17281 aggtcgttgc tgtttcgttt tgttgtgtcc tggcgaacgc cgcttcttgg gcgggcggcc
    17341 ggaaaccggc gtcgcctttg gtggaggagc gacccgtgac ggtggaggac gaggcggcgt
    17401 ctcccgttcc cctgcccgaa gagacgcgtc ccgcggtgga tgcgcattcc cttcccggca
    17461 tgcggcccgc cggggtcgag gtgtccttcc tctccccgcc tgtttcgcgg tcggcgaaat
    17521 atcagcgggt ttggatttgg caggagacca acgattgcct ttggaatatg gcccgggagc
    17581 attacgggga cccgtttttg tggccgagga tttacgaggc caaccgtgat ttgatccggg
    17641 accccaacgt cattttcccg aaacagcaga tcgtgattcc gccgttggaa acggcgcagg
    17701 tgtatgagcc gccgatgctg cccccgccgc ccccgcccgt cgaggaaccg gtggcggtgg
    17761 aaccggcgcc tgcgccgcct cctccgccgg agccggaaaa accggccgaa cccaaggcgg
    17821 acggcgaact ttggaaggaa aggcggcagt tcacccaatg gtgaccaaac gcatggcctt
    17881 gcaggaccag gaggaggcgg ttttcctctt cggtccgcat gataaaaatc tgaaagaact
    17941 ggagcggcgt ttcggggtcc aggtcttcgt ccgtccttct tccggcgtcg gccaggaggg
    18001 gcttacgctc gcggtgcgcg gccggtccgg ttcggtggac catgcgttgg cggccttgaa
    18061 ggagatgaag cgccgcctcc cccattcgtc cgcgcctccg gcgccggagg cggcggtttt
    18121 gctcgagtcc aacgcgttcg acgagaagac gcatcccgac gccattttta tgaccatcac
    18181 cggacgtccc atccggccga tgacgccgca acagaagctg tacgtggagg ccattcgaac
    18241 gcacgacatg gtgatggcca tcggccccgc cggcacgggg aaaacgtatt tggcggtggc
    18301 ctcggccatg gccgcgtaca agagcggccg ggtgtcccgc atcgtgctga cgcgtcccgt
    18361 ggtggaggcg ggagaaaagc tgggattcct gccgggcgat ttttattcca aggtccaccc
    18421 gtatctcaaa ccgctctacg acgctttcta cggcatggtg gggccggaag agttccgtct
    18481 tctgcgcgac gaggagagca tcgagatcat cccgctggcc tatatgcgcg gccgcaccct
    18541 ggacgacgcc ttcatcatcc tcgacgaggc gcagaacacc accccggaac agatgaagat
    18601 gtttttgacg cgcatgggga acggctcccg cgtgatcatc acaggggaca tcacgcagat
    18661 cgatttggaa gataagtccc agtcgggctt ggtgcagata tccagcatcc tcaaacacgt
    18721 ggagggcatc ggctttgttt ggttcacgga gggggacatc gttcggcacg ggttggtgaa
    18781 gaagatcatc cgtgcgtaca acgattggga aaatcgacgg aaaggcgggc atggttcgcc
    18841 cgccaaatcc tgacatgagg aggatatctc ggcgatgatc ggccgttacc tgagcacgcc
    18901 cctgcggcac gtgcttctga ggattctttc gtggaccgaa aaacacagcg agaccccgcg
    18961 gctccagcgc gtgccgctgt tgaagcgcga ggtgcgcatc tccaccggct gggtggcgct
    19021 cctggtgtgg gcgatgcttt tggttgttct gttgttccag ttcggcctgg agtggcagaa
    19081 cgcggttgcc gtattccttt tcaccgggat gatcatggtg ttgttcatct tctatttccg
    19141 gcacgaccat ccggagatcc tccacgacga ggaagccgtc atgctgttgg gcgttctggc
    19201 ggtgggcagt gtgctgatga tccagctgtg gttccagatc gtccgttccc tgccgtgggt
    19261 gtcgcccttc gggatgccgt tggcggtcac gtccatcctg accgtgctcc tgctccagcc
    19321 ccgcctggcc atcatcctca ccaccgtgct cagccttctg ttcggggtca tcgataattt
    19381 ttccatgacc ggcacgttgg tgatgttttt cggcggtttg accggggtgg cccgcgcgct
    19441 ttatgtccgc acgcgtcgcg acgtcacgcg cgccgggctg tgggtctccc tggctcaggt
    19501 gctgtccatc ctcgttttgg gttttttgcg ccattggccc gcgcaaaaca tttttttcgc
    19561 gctgctctgg ggggccgtgg gaggggttct ctcctcgttc ttggcgttga gtctgttgcc
    19621 gtatctggag agtttcttct cccgcctcac gaacatcaaa ttgctggagc tggccgacgt
    19681 gaatcatccg ctccttaagc gcatgagctt ggaggcgccg gggacctacc atcattccct
    19741 catcatggcg tctctggccg agcccgccgc tgaggcggtc ggcgccaacg gtcttctttg
    19801 tcgcgtgggg gcctatttcc acgacatcgg aaaactggtg aaacctgaat attacgtgga
    19861 gaaccagggg gcgttgggga atccccacga accgctgcct ccgaatatgt cccgtttggt
    19921 gatccagtcc catgtaaaag aggggatggt cctggcccag cagtacgggt tggacaagat
    19981 catcgtggat ttcgtccgaa tgcaccacgg cacctcgcgg atcgaatact tctaccgccg
    20041 ggccatcgag cagagcgccg acctcgacga tctcggcacc gaggaatacc gctacccggg
    20101 tcccaagccc aattcccggg agacggccat cctcatgctg gcggattccg tcgaggcctc
    20161 cgtgcgcacc atcgactccc ccacgcacca gcgcctgcaa gaccaggtag ggaagatcat
    20221 cggcaccaaa atgaacgacg ggcagtttga cggcgtccct ttgaccctgg cggacctgca
    20281 tgagatatcc gaaagtttcg tcaatacgct gacgggcatc taccactctc gtatccccta
    20341 tccggaagcg tcggaagaac ctgagctctt tcctccgccg gcgtccaatt ccctcttcag
    20401 aacttgagtc ttccccgccg gcgccgaccg tcaaaaaagg gccgcgcccg ttctctgcgg
    20461 cacatccgct tcgcgcgcga ggggggcgtt cgggccccgc tgccgctccg gctggctcgt
    20521 cgggcggttt ccgagacgct ccgttcggcg ggccgacggg agcccctcga tgtgaacgtg
    20581 gtgtgggtgt cggccccccg cattcgggcc ctcaatcgcc gttttcgctc cacggaccgt
    20641 ggcaccgacg taatttcgtt ccgctatgac gccgataaag aatttccatc gccggcggtt
    20701 tcgggcgacc tcttcgtctg tgtgggacgg gctcgataca atgcccggcg ttttcacgtt
    20761 tccgtcgaag aggaggcggt gaggctcctc gtgcacggca cgcttcacct tctgggattt
    20821 accgactatg tccctgtcga gaaaaagaaa atgtggcgga gacaggaagg aatcgtccgt
    20881 cgtctcggtt tcggcgacgc gttcccgcag atatgattgg gatgagtttg atcgtcctcc
    20941 tttttttgtt tctggccgcc gcattcatat cggtggcgga ggcggccatt atttccctct
    21001 caacgctccg gatgaaacgg ttgatcgttt ttcatccggc cctgacggcg tatttcagcg
    21061 aatggctcgc gaaaccacac cggctcattt tcaccctcat gtttttgagc aacattacgc
    21121 tcatcgcgat ttcgagtctc tcggccgcca tggtgcgaca tttcgattgg ggcggcatcc
    21181 cgctgggggt ggtggaagcg gtggtgtggt ttgtagtgac cggccttctt ttggttttcg
    21241 gggaaatcgc gcccaaaatc attggtcgtg cgctgcggga gaaggtggct ccttggatag
    21301 tgccgatttt cggcgcactg acgcactact tgacatttct gttcgccccc ctcaactggt
    21361 tgattgaaaa agcagccccg cggttgcagt tttctcccgc agggcagttg accgtcgtga
    21421 gtttggagga atttcagcac atcatcgggg aatcgcaggt ttccggggaa gttcctttcg
    21481 atgccagcga gatgatgcgc cgggttttgg cgttgccgca aaagacggcc gcggatatat
    21541 gccagaaaaa agaaaacgtg gatgtggtcg ccctggaggt ccgaaagaag caccgagggg
    21601 atgagctttt cgtggacctc cttgtcgaaa ccggtcgaag tcgtgtcccc gtgacaaggg
    21661 acggtgcgtt cgtgggctat gtgaatgtca tggacattct gcagcgttgg cggtccggcg
    21721 aggtgacttc ggtggaggaa cttattcgcc aggcccccgc ggtgtcggca tcccggcggg
    21781 tggtggacct gttggaggag ttccgccgga gcggcgagaa cctggctttc gtttttgacg
    21841 accaaaaacg atttttcgga ttggtgaccc tggaggacgc attggaagag atcgtcgggg
    21901 aaatcctgga cgaatacgat ttggaactga aaaaggggga ttagtcgggg tatgatcctg
    21961 ttttggggcg ggttgttgat gggcgtgttt tttttgacga tgtccgggtt tttctccgct
    22021 tcggagacgg ccctgatgag cctgtccaag atccagttgg gacggttggg gaaagtttat
    22081 cccggccgtc tgacgttttg ggaaaaagac gccgatcggg tgctggcggt catcctcctt
    22141 gccaatacgc tggtgaacgt ggctttgggt gttttggcca ccgctttggc cgtgcagatg
    22201 gaaaaagtgt ggggagtgcc gtttcgttgg ggaggatggg ttgtgccggt gatggtgggg
    22261 gtgttgttga tatttgtcgg cgaaattgtg cccaaactgg tcgcccgcgc ttacacagaa
    22321 tcactggcat tgaaggtggc tgtcccgatg cgtttgatca cgcgggcgtt ggggccggtg
    22381 gttcaggggt tggtgtggat gattggtcgc gcgttggcgg tgatctcccg gtcggtgcgg
    22441 gcggaggggg ggcagtggaa cgtccgcgtc attcggcacc tgttggataa gaccaccttg
    22501 gcgccttcgg ttcggtccgt tctcaacaac atactcgatt tcggaaacct gccggtgagc
    22561 cgcatcatgg ttccccggga cgagatattc gcggtggata tcgccctgcc gaaatcggag
    22621 ttcgttgccc gtgttctttc ctcgggatac tcccgtgtcc ttgtgtataa ggggaacctg
    22681 gatgagatcg tgggggtcgt gtacaccaag gatttgttgg cgttttggcg gggggaatcg
    22741 ctgatcgtcg tcacggacct cattcgtgcg gctttccggg ttacggaggg cgtgccggtg
    22801 gcgcaacttc tgcgtgaatt ccgccaaggg aaataccaca tggccgtggt gctggatcgc
    22861 aaccggcgtg ttcaggggct ggtcacgctc caggatattt tggagaccat cgtgggagat
    22921 attgcagagt ctcccgaact cggcggttcc tcggcggttc tgtgaaacta ttctcccatt
    22981 cgcacgcggc tcttgttata atttttcgga aaaaccatgc agtttaacgt caacgccatc
    23041 gttttaaaat ccgagcgggt cggcgagtcc gacaagcggc tttcgatcta tacgcgcgag
    23101 cgtggccggt tgtgggcgtt ggcccccggg gcggcccgcc ctcgcgcccg cctggctccg
    23161 gccacggaac cctgcgtgga ggcgcgcttc cgtctttggg ccattccgga caaccctttt
    23221 gcgcgcatca ccggcggggc cttgacgtcg gctttccccc tattgagggc ggactgggac
    23281 cggatgacct cgggtatctt tctttgtgag tggacggatc gtttgacgcc gctggaacag
    23341 acgcatgcgg ccaaatacga gctgttgcgc cgttctctgg cggctctgga atggagcgat
    23401 gatcggccgg ttgtcttggc gttcctgacg cagttcctgg aacaggcggg gtatgtcgtc
    23461 ggtgacgagg tgctgggggc cgctcaccat gcccggtttc gctcccttgt ggcagatctc
    23521 cgcggctatg atttttcgac gcccttcccc gaacttgacg agggggtgga cgggttggag
    23581 gagcgtctgt tgaggttcgt gacgccgctt ttgcgggaac cgcttaaaac gctccagcat
    23641 cgaaagaatc tgcggatgtt tatggagaag acccactccc caacggcgga acccgcgccg
    23701 gcacggaact gattttggaa aagccatgac atttcaggac atcatcatca cgctgcataa
    23761 attctgggcc aagcaggggt gcctgctttg gcagccgtac gatctggaaa agggggcggg
    23821 caccttcaac ccggccacgt tcttgcgttg tctggggccg cagccctggg ccgtggccta
    23881 tgtggagcca tcacgccgcc cgacggacgg ccgctacggc gagaacccca accggcttca
    23941 gcactattat caatatcaag tcatcatcaa gcccgcgccg gcggacatcc agaacatcta
    24001 tctttcctcg ctcaaagcca tcgggatcga ccagcggcgg cacgacatcc gttttgtgga
    24061 ggacgactgg gaaagcccca cgctgggcgc ttggggactc ggctgggagg tgtggctgga
    24121 cggcatggag atcacccagt tcacctactt tcagcaggtc ggaggcattg acctgaaccc
    24181 catttccgtg gagttgacct acgggctgga acggctggcc atgtattccc agaaaaagaa
    24241 cagcgtctac gacatcttgt ggacggataa gatgacctac ggccaggtgc atctggagga
    24301 cgaacgccag ttctccaaat ataattttga gtccgcggac gtggagatgc tgcgccgcca
    24361 tttcggcgat tgggaaaacg aggcccggcg gctgctggcc gaaaagctgg tactccccgc
    24421 ctacgacgcg gtgatgaaat gctcccattt gttcaacctt ctcgatgccc gccgcgccat
    24481 ttccgtcacg gaacgcgtgg gctatatcgc ccgcgtgcgg gccctggccc ggggcgtggc
    24541 cgaggcctac gtggcgatgt cgccgtcgga ggaggggcag aaatgaaacg gccgtctttc
    24601 atcgcgatgc gtgagccgga acccagggat ttccttctgg aaatcggcgt ggaggagttg
    24661 ccggcccggt tcgtgccgcc ggctttggac aatttacgcc aactcgccgc ggcccagctg
    24721 gagacggccg gcctttctta cgccgccgac ggtctgcaga catggggaac tccccgccga
    24781 ctgacgcttt tcatccgggc cctggcgggc aaatccaagg atcaggttca ggaaaatatg
    24841 gggcccgccg tggcccaggc caaggacgcc gatgggcatt ggaccccggc cgctcagggg
    24901 ttcgcccgtt cgcagggggt ctctctggaa aagctggagg tgcgcgaaac ggaaaaaggg
    24961 ccgcgcctgt gcgccgtgcg ccgcaccctg ggcgttccga cggaaaaaat actgccggag
    25021 atgctttcgg ccgtcctggg aaaattgaat tttccaaaaa acatggtttg ggagaagagt
    25081 cggttctcct ttgcgcgtcc tctccggtgg ctggtggcgc tttacggttc tcagccggtc
    25141 aaattcgaga tcgccggtgt caaggccgga cgcaagacgt acggcctgcg cgtccagggc
    25201 gggaaatctt tcgatgtcgt ctccccggcc aaatacggga tgctcctcaa ggaccggtgt
    25261 atcgttgtcg acccgtccga acgccgccat ttgatcgaga aacagatcca tcaggccgtc
    25321 aaagacgccc acggcaaggt gcggttggac caggacggag cccttctgga cgaggtggtg
    25381 aacctggtgg agcatccggt ggccatcctc ggaaaattcg acgaaaaata tctcgcccta
    25441 ccgccggaag tcctcgtcac gtcgatgaag aagcatcaga aatttttccc ggtctatgcc
    25501 gccgatggcc gccggcttac ttctcgtttc gtggggatac ggaacggggt ttcggaaaac
    25561 caggcggtgg tgcgcgaggg gtatgaacgc gtgttggcgg cccgcctggc cgacgccgcc
    25621 ttcttctatt ccgaggataa aaagaaaaaa ctcgaagata atctctcgtt gttgcagggg
    25681 atcacttttt tagccccttt gaatttgttg aacaaaacaa cgcgggtcga aaaactgatc
    25741 gatggccttc tcccgtcttt gccgatgggg gacgacagaa aagaatgcgc aaggcgtatc
    25801 gcgcgtttgg cgaaaacaga tcttcttacc gccatggtgg gggaattccc ggagcttcag
    25861 ggggtcatgg gacgaattta tgcttccgaa cacgagaatc cggaggtggc ccgggggatc
    25921 gaacagcatt actggccgtt ggccgcggag ggagacctgc ccgactcgga ttcggccgcc
    25981 gtggtttcgg tggcggataa attggattcg ttggccggca attatttggt tgggaaagtc
    26041 ccttccggct cccaagatcc ttacggattg cgccgggccg ccgtgggcgt cttgcggatc
    26101 gtttccgacc gccgctggag aatgaatctt tccgatatcg cgcttgccgc gatgagggaa
    26161 tatccgtttt ggaatgagaa agtcggaccg ccggcccacg cgcagcttgt gtcgtttctg
    26221 agacagcgct gggcggcgct gatggcggcc gccggtttcc ggttcgacga agtcgaggcg
    26281 gcggcctcgg ccgggttcgg cgacgtggtc gaggttttcg cccggctgaa agcgcttcgg
    26341 gacatccgtt cccacgccca tttcgcctca ctttccgtgg cgttcaagcg ggcggacaac
    26401 atcttgaaac aggcggagaa acaggcggtg aagggctacc atatcccccg gcccatggac
    26461 ggccggagca tcctcatccg ggaagagctt ctgcgggacg gttcggagcg cgcccttttt
    26521 tccgccgtcg tccaatgcga ggaggccaac gcccctctcc tggaggcggg cgactacgcg
    26581 gccgcctatg ggaaccttgt ggccct
//
LOCUS       DAOTFD010000014        26564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1195_length_26564_cov_4.769475, whole genome shotgun sequence.
ACCESSION   DAOTFD010000014 DAOTFD010000000
VERSION     DAOTFD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 26564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26564
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_26564_cov_4.769475"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="P5079_01790"
     CDS             <1..568
                     /locus_tag="P5079_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28750.1"
                     /translation="ALSVEEYLQELQMEVVVRSVGEETAARDAQLTQKTNQFNLTTRR
                     YTESDIRSFFESRDTFSLFCLHLRDRFGDSGIVGVAILRKEGDDAATMDTFLLSCRVL
                     GRGVEGAFLLICAKSCVRRGCRRMYGLYVPSAKNSQVKDFYIQKGFRNEGTGEDGAQR
                     YLADAAELSRRAVPRYFRSVTLDGEEVK"
     gene            568..798
                     /locus_tag="P5079_01795"
     CDS             568..798
                     /locus_tag="P5079_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY28751.1"
                     /translation="MEEKLRKVMAAALEVPEARIGPEATTETIETWDSVQHLKLVFSL
                     EDAFRVKFTGTEIIELTGYGAIREHLLKKGAK"
     gene            818..1717
                     /locus_tag="P5079_01800"
     CDS             818..1717
                     /locus_tag="P5079_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HRY28752.1"
                     /translation="MAVEVLSRKNFIAALRRAGIREGDVVHVQSDLRTPGPVEASPAA
                     NPREAILEFYLAGFREALGPSGTLTVLTSFEDYGRFGTPFVREESPSRAGAFSEYMRT
                     QPGAVRSVHPIVSLTGLGERAEELCGGNHYDGCGYDSPWGRLHRLNAKFVSLGIGMRI
                     AFSFQHYIEKQFGIPYQYTKLYRTPVYSNGREVPGPFTMSVRYLDFGIAYDFSRFEKR
                     LLEKGAAVEVALGRAMLQLTTAQPALDVGMACLNEDRYFFLKTPPTFRDGEFPLDGFT
                     GPEKYMYDRGKNDAPRAEEKPHG"
     gene            1710..3041
                     /locus_tag="P5079_01805"
     CDS             1710..3041
                     /locus_tag="P5079_01805"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HRY28753.1"
                     /translation="MVDLKKLDRLLQLNIGISQAANREMMDIFNNVTPIRVRRYPAGR
                     EYNGWVVPHDWVVKKAHIRKKGKILFDGTVHPMAVAGYSSSFHGTVSKKELDKHLFTK
                     KEFPAAYPFHSIYNYRPWGKHWGFCVPYNVCKKWPAGDYEIDLETEFKEGEMLAGECR
                     HPGELKDTVVFNAHTCHPCQANDDMAGALVVLELFKWLATQKTRYSYLGVLAPEHVGT
                     VFYLSDFSDKEVAELKLGCFVEMPGTKTPLVLQKSFEGNTIMDRVAKYVLGQEQPRLR
                     VGEFRTVVGNDETVWEAPGIEVPMVSISRWPYRQYHTSDDNVKIMDPERLEQFLECLK
                     RIVRVFEDDRTIERRFRGLVSLANPKYNLYIERPDPVVDKGLSPMDLRFGRMQDPLPR
                     YFDGKHTVFEISEKFDVPFEKLSAYLREFERKGLIKLQPPPSLDHYKPAGA"
     gene            3115..5013
                     /locus_tag="P5079_01810"
     CDS             3115..5013
                     /locus_tag="P5079_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28754.1"
                     /translation="MIENKEAVVSPGTLVLAMSSRVGERLRQAGVPYRIPEEFIAPAE
                     LTRRGMENFKWLERFCHYSDRWLAAHLERIRLHALTPTAWNYSALKNIFDPFFLRTLE
                     VKAILESQRPEEVVYFEVPAVAALDDYVDNRESLSAAVLPRVADRLKIAKLSPLPAVE
                     SAPSGEGRRVDLRGYARRFFWARWARTLQKYYGAAGFLPAALSGFRKKDCVLALNVDY
                     DLFPLFREASARRTFRVLFWEPGSEHAPRFLCPPAVFPAFRGKRPDRRFMEETSVDWK
                     RLTDDKEYRSFFVIDGVDIFPAVEKQLRHFYEDILRRSVWSFFEAERVLDKFRPRAVV
                     TECLSRPQDAAVFEAARRRQIPRVIYSHGPYGMPSTPVTDYLDYKRTDYFLAWGEGGR
                     KDVERYPAPNPRVEVVGSGALDALKERPRLDARGRAALCRSLGLEPGRRTVIHVLGPI
                     SENGYRVGVDFNDRDYFTFDVEKKIVKAFQKFPEAQFLLKLFPFQGMTDPIVDFARHE
                     GFRNLRFVKWTPFVKLLDLADLVVVEVYSTPLMEALAAGKPIVMFTGQESPLESLGDV
                     SEAVHFAGNTDQLERLLRSFLRGDPAAFPRKNGEAFLRKYGTHLNDGRSAQRALEALK
                     TIALGQEG"
     gene            5019..6509
                     /locus_tag="P5079_01815"
     CDS             5019..6509
                     /locus_tag="P5079_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY28755.1"
                     /translation="MSLAVNIITYMSTGVVVRAITLVTNLVFRRILGPEMAGIWNLVY
                     IVTGYLSTVSIGVQTGSERDIPYWRGKKNAEMERRLVHQMFTVSFLEAAMVGSVFLIY
                     LFFFGGKYRHDLYIGFLSAAGYVVLWRMMGCFVVGFRSIQNFTFLGKSEVVFAVLDLG
                     LIVGLTSFYGLYGQYLAHALGFLLKLSFWYLWTHKRKMFDIGWHLVLADIKPVAAVGF
                     PWLLSSFVWQFMMSVDSLLVAKMAGVTALGYYSLGVSIVRMLTEIPNTVSTVIFPRLL
                     EKFGEREDIRPIKEDVVRYLKNLAFVVIPLLFPLAYFFFPWVVRQFLPKFLPSIPAAK
                     VLLLAALFMPMIHMPGQILILQKRIWLYVGLQLGAGIATAVLVLLGVRGGIVMVAAMA
                     VLSQFLFFSALFMVSFARADLPKNVFSNYAGLLLPGLYCGLWVFGVDQLLLRWGAFGT
                     WFSDLLSCGGGVLLSYTGMSVLFLKAERELGIWRHVVDFGQKLKTL"
     gene            6526..7740
                     /locus_tag="P5079_01820"
     CDS             6526..7740
                     /locus_tag="P5079_01820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY28756.1"
                     /translation="MRKKIAYFVDTNALGGGEKYVVDLMAAMGKRGHEVLFLCQQKEE
                     LAAYVGRMCPGVRIVAARFPSVSRSAAIQAGLAINRRSNKIFEWLKIPGLAVYYLNVL
                     YGFIVLWRLLRRHRPEVLHVSNGGYPAAESHRAAVLAARWVGIPKRVMTVHNMASGYP
                     PVACIEKRLDRWVEGSLHAIISVSQRAKEALAVQRGFDTGHVSVIYNGIVPHRVSEAE
                     GLRRELGIPPGVKVVGNIGGLQPRKGHEALLEAFKKVHAEFPRTVLVIIGEGPCRSAL
                     EAQVRAAGLETVVLLPGFRPDAGRYADMMDVFVFPSLAYESFPYVVLEAMSAGRPIVA
                     TDVGGTAEQIVDGQCGRLVPAGDVSEMSRAIGRVLSDENTAASWGVAAKKRVEELFTL
                     EGMIEKTEKLYL"
     gene            7784..9610
                     /locus_tag="P5079_01825"
     CDS             7784..9610
                     /locus_tag="P5079_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HRY28757.1"
                     /translation="MNRYRGKELFPYLRHVAGRGTFVAAFRAGWEAVARRRGSPCAPP
                     WMAFPPKEADVTVSASGVQAAIWGPLWPIHAGVPLPEPLSVPLATKNHDLGDRDLWRT
                     EFEDIEDTFSLHRFGWVPVCLGRHGTIPLGMVLWQRVVQWIREVTPSQFPSAWETYSA
                     CERIVHWSQLYAWLRRAAGRGLPEEEAFVESLLLHGEYVLSHLEFYGEDDSSKTNNHL
                     INNGRALYVLGAFLNVPRYLDTGRRLLADGMRRSFTQAGFLREGSSHYHILLVSRYLD
                     VLEVARNIRDEAFVEELGPFLDRSMAAARFFLPEKEGVFPLIGDVSPDFDPEWTLRAV
                     EDAARRLLGAGGGLYGNVASEGKGVTDRLRDGWCRYDDPNYRVYWHVQPGGAIPPYSH
                     AHHDTGSFELSVERHPLLVDTGRVSYVADAAGLYGRSAAAHNGVLVDGREPFVSHWLN
                     GGRFLMSDYGRTEVRTWWENASSRPAFVVEHQGFSRLRGIGKYRRRFLLDKDEIVLED
                     RYEGRGRHVLETFFHAAPSVGVAVEGNALSFREKGTRRNLELALDAGNLAGLSLMRGE
                     WEGGRPMGWFSPRYGRNVPTTTVRFRHKAVFPFTNRFVLRLG"
     gene            9614..11494
                     /gene="asnB"
                     /locus_tag="P5079_01830"
     CDS             9614..11494
                     /gene="asnB"
                     /locus_tag="P5079_01830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY28758.1"
                     /translation="MCGIAGIFHKEGDGADRNAVRAMCRALTHRGPDDEGFFDSPGLS
                     LGMRRLSIIDLKGGRQPLFNEDGSLVLVFNGEVYNYIELRRELLSRGHTFATQSDGEV
                     ILHLFEEKGLDALQELNGMYAFCLWDVRKKEGYLVRDRLGIKPLYYHVTPESRRLCFA
                     SEIKALLHAPVEKEVDMEELFRYLAYMYIPGCGTPFKNVRKLQAGSFLRFGAEGIGAP
                     RRYWTMPAGDPVEDEGRLKEEFFSLLESSIELQSRSDVPVGVFLSGGLDSSLITAYFS
                     KKTKSRVLSFNVKYEGAPFDESRYAELVAEKFGCEHKSVNVTPDDMLKELPRLVWHMD
                     EPHADHAMVAAYKVSELAAKDVKVVLNGTGGDELLGGYPRYRPNAWPLAAWHKSPEFL
                     RTLAVGMAERIPFGGDFVKKARFNGTPDDTYVWRLRQFKPYHLERFCADRPVAYTRDA
                     RVREWLDRAPTGDLNRYLYLDANFYMVDDLLLLLDKMTMAASIEGRVPLLDHRLVEWA
                     FKMSGRWKVRGGETKLAAKRWLRGILPEEILWRPKLGFGAPMREWMKDKLFPKAAGLV
                     AARPPARENLFWGLRGEALRERMRRLNFQQNYALLALEVWFRLYIDGESIAEVTERIR
                     KS"
     gene            11491..12726
                     /locus_tag="P5079_01835"
     CDS             11491..12726
                     /locus_tag="P5079_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY28759.1"
                     /translation="MKTVLMLVYFFPPSSGAAAQRSRKLAKYLPKHGWQPLVVTAGDD
                     PFVPRDNTLMEDVASTEIHRTLPSILPGMLLGRRALRLLLDRFFVPDIQVGWIPRAHS
                     CVKKLLAGRKIDLVYTSCAPFSANLEGERIGKRYGVPWVTDFRDPWVAGTTFRPPTPA
                     HRWIHAALEDRAFSRCDWFVANTPGFLDEERERHPVLRQKSCCISNGYDPEDFPSVRP
                     AGGKETWEMVFTGSWHRRYYPDTLFRIVRFFLERNRTAKIKIHYSGPHFKQFFVPAER
                     NGLAPLLEDHGHLSYKRTIALLQSGDLLLTTLPDDARARTWVPAKLYEYLATGRAVFG
                     VCPDGDAARLVELAGSFVRPNALDAETRGGDILQGLWDDWKAGKRRETARAVLEKYQY
                     PVLAERLAGVFDRLTEKMG"
     gene            12723..13835
                     /locus_tag="P5079_01840"
     CDS             12723..13835
                     /locus_tag="P5079_01840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY28760.1"
                     /translation="MSRKKVLHLTVGLGLGGAEKLMAEMAARLDPSRYETQVLALKGE
                     GPLGDSLRARGVKVSALGGRGPWDVRVLPRAARFLREVRCDIVHSHLLWANLLACLLK
                     GRSKVVWHEHDMDDWMGRPQKTAERLLIGRADTVIAISRAVADCVARREPFVRDRIRI
                     LHNAIPLKEKPTSPQGKRSFRAALGFFGEEKVVGYVGRLDEPKKGLKVLLHAAAEIAA
                     EDPSVRFVVVGDGNARAALEAEADRLGLSNSVRFWKARENVEEVYGALDVFVLPSLWE
                     GFGISLLEAMDEGLPVAGSRVGGIPEVVVGGETGLLSPPGDVEALAVNLRKLLADPAL
                     RRRLGSAGRARVQAQFGISKMVEELEKIYDETEKTQ"
     gene            13832..14476
                     /locus_tag="P5079_01845"
     CDS             13832..14476
                     /locus_tag="P5079_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY28761.1"
                     /translation="MNETALKKWSRFHAPMGCSVGGVDGRVEALLRGRVLNIGAKAWG
                     RPEWIPLDLVSNDPRTALLADAADLPVRSGVLDGAVLRFVLEHVPSAERVVAETHRAL
                     RPGGLLFVTVPFVEPYHADPQDFRRFTPEGLKKLLENFEMLECGTYYGPASALVEFLR
                     EFTASFFDSVFLKKGVRFLAGWTFFPLKYLDVFLGRKKFSWMCAYSLYAVAQKR"
     gene            14543..16027
                     /locus_tag="P5079_01850"
     CDS             14543..16027
                     /locus_tag="P5079_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY28762.1"
                     /translation="MKILLINPITRLNNYVSNLPLGIAYVAASLRQEGHDVRIFDLDA
                     HKYGKEDVRKTLEKCDYDVLGMTGIITSFQQQRWIIDEAKRVNPATPVILGGVGVSSI
                     PELFFQQTKVDVVVCGEGDIAIREVLARLSDKKGLADVPGIHYRQGGGVVKNPPRSVV
                     RDPDTLPFPAWDLLPIEKYLCRPSPAWHKKNMTFLTARGCPYQCTFCQPTGGHLYRYR
                     SIDNVIAEMKKAMGLYHYGHALLSDECWPIRRDRCFEFCEKVKPLNIEWSCTGRANLM
                     DEPLVEAMADSGCVMVGMGYESASHIILKNIKKSQTAEHERKAIRLLQKYGIVPSTSY
                     IAGLPGETPATLRETVDFIEDLDVPIFDFFFATPYPGTALYDYAVTRGKITDPLPLVE
                     SYEEMASNLLVNVTDMSDEELIRIKREAEVELREVYRRRHPLYFHLERLKGFYERLLA
                     RWRSGSLPKVFLHRLGRFLRKWGIADSDRLLRSGEYSWLSDKRV"
     gene            16024..17148
                     /locus_tag="P5079_01855"
     CDS             16024..17148
                     /locus_tag="P5079_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRY28763.1"
                     /translation="MNQPKEEPKEGASAAGSAVFIKEAAAWDRFVSGSEKPHIMQSWA
                     WAELKAKSGWRPHRLLIERRGGARVGVSVLERDFPKIGRRFFYAPRGPVVATGWRPED
                     LEQLFLELAGLAKNRRAVFLKMDPDVSLEQDWISSDLRGAGFVPATGGGGFAGVQPRC
                     VFRLKIGASEEELLKNMDQKTRYNIRLAGKKGVTVRPVSDRAEIETFHRILIETGQRD
                     GFMVRPLRYFNDIQDLMGPRGQAQYFLAELGGKALAGALALRFGPVCWYAYGASSNEG
                     RQHMPNHLMQWTMIRWAKSQGCGLYDFRAVPDDPNPQNPLYGLYRFKKGFGGDLVRFV
                     GEFDKVYSPWWYRMWAYGWPVYKSVRKFMRGPARKAGRNE"
     gene            17145..18212
                     /gene="alr"
                     /locus_tag="P5079_01860"
     CDS             17145..18212
                     /gene="alr"
                     /locus_tag="P5079_01860"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY28764.1"
                     /translation="MKPLRWVEVDLNAVGHNVKSVRRRIGKKTGFTAVVKTDAYGHGA
                     VPVARAALRCGADGLAVTYLEEALPLRRAGIKAPVLVMGPVSPAAAPLVRRWNLSVMV
                     DNEPLLKALKRAGTASHPIGVQVKVNLGLWRWGIPLPRLGFFCREVDAAPSLRLEGIF
                     AHPGYMIGKNKSRIEDQLRNFVAAADACKNELRRSRPELHAADSAVLLDFPEFQLDRV
                     RVGNLIYGINPTDKPLELRNPWRALSRLVRLERLAVGDMVGYGGEFVAPQAMTIATVP
                     VGYGHGLTLEPASRWIQLKSGQNYWGKIRGIVCPFVGRVGMSHCLVDVSSVPAPKIGD
                     VIQLPLRRTASLGWPKIYSSQ"
     gene            18327..19298
                     /locus_tag="P5079_01865"
     CDS             18327..19298
                     /locus_tag="P5079_01865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY28765.1"
                     /translation="MYVVALRRVRRFIAWGSKAVLKTLLVLVIAEGVLWATGHLYLWY
                     YTKFAWKPADLSPGIRRILFIGESTVEGQRCDEGDFKGPFPAQLEAMLNRMSGRNEFS
                     CINHGISGVTSAHMLRYIGKDLVDYAPEIVVLQAGMNMNMPSIFRLIRRAGWDIEVDS
                     VVLAKLHRLRIVRFGKLFYEMGRAGDLDPIHYYEDLELENAENKANIQTVIDKIKKYG
                     ARVVICNYFKEGYANDILKGVVRENGLILCDNAAVFKEWQRRGVDRTIISSDGWHPNG
                     NGYRLIACNLLKTFLEKDLVPYAVEPSKAERIKNLEAVLEGNLAAEI"
     gene            complement(19346..21589)
                     /locus_tag="P5079_01870"
     CDS             complement(19346..21589)
                     /locus_tag="P5079_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRY28766.1"
                     /translation="MDGMLATFRRLSHKVRVTLLLLTFYCLLMTWAALTFRFYQAQLP
                     PIGSLESYTPSLITRIYDVRNELVSELYVERRTVVPLPNIPLDFQRAVLAIEDRNFYD
                     HWGVDIKGILRAFLANLRAGRTAQGGSTITQQLAKNIFLSHARTLNRKIKELLLTLEM
                     ERHFSKDEILELYLNQIYFGHGAYGVDAAARVFFGKNVADLTLPECALLAGLPKAPHA
                     YSPFKHPARAIKRRQLVLDRMREAGYISEKEELQACGAPLNTLKTPLAPAMAPYFVEY
                     VRLQLEPKYGSTSLYRQGFSIYTTLDLRMQKAAEETSEFHLSSFDARYAEQRLLYLVK
                     NKKIPESYYIDWKKRKEREAAGEETEVPDENAEPVNVQGALIAIDPRTGGIRALVGGR
                     DFQKSQFNRAAQARRQPGSTFKPFVWLAALESGFTAATVIEDYPLAFTDVERQPKLVA
                     ESTDYAVLREMVTGYYPPDMDPDAPDPVWAPKNWDDKYLGPVTLRKALALSRNLVSVR
                     LIDRVGPRTVVRFAHNAGIRSPLDAVLSLGLGSSVVTPMELVGSFATFANNGVHMEPF
                     AVQKVLDNGGKLLEEHVIQGNVAVSPQSNYLLTRLLHAVVEEGTARYARRLGRPAAGK
                     TGTTQEMRDAWFVGYLPDLAAGCWIGYDDFTPLGKGISSAGTAVPWWTDFMLESMKYI
                     PARDFPVPPGVVFAKIDAETGLLALPSCPHVALEAFRQNQVPQNFCDVNHEKLLPLIS
                     AETEIVE"
     gene            21714..23351
                     /locus_tag="P5079_01875"
     CDS             21714..23351
                     /locus_tag="P5079_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY28767.1"
                     /translation="MRLTLFLAVFLFIAYGRYSHRLPPLLLLGLLGLFLFLAGLQFFV
                     SERFFRSHYFQAAVFLSEVTLLSVSIYLTQGFSSDLYLVYFLTILMAGFSRQVWQVMV
                     VAVASSAFYGFTLFVANPEMPLWDPGLLLRIPFLMLVAVFVSFYSMTVQMKSVQLEKT
                     TKELTQVKEQLTMAEKLSVMGQVISGVAHELNNPLTGIMGFSQLLLREEAVENDSKLR
                     WQIENIFKESERCQKIVKNLSTFARQHKPEKTHIGLNGILEDCLRLEAYQLVTRNIQV
                     KKDLFPELPKTMADFHQLQQVFLNLIINAQQAIGESRGKGTITVSSHQVGNMIRIEVE
                     DDGPGIPSENLAKIFEPFFTTKEPGKGTGLGLSLSYGIVKEHHGNIWAENVSSGRGAR
                     FVVELPILEGSAPSHSGEGRVAAAPPAAPATGKRILVIDDEQIILELFHRFLRHLGHE
                     VDVARDGDLAMQKLRSNRYDSVFCDIRLPGLDGIRLFRWVRENRPDYVPTWIFITGSV
                     TSAMEGALKESSRPVLSKPFTLQAIETALRETLKVSS"
     gene            23604..23981
                     /locus_tag="P5079_01880"
     CDS             23604..23981
                     /locus_tag="P5079_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28768.1"
                     /translation="MADEKPKKPRVVWADDDLSLQIVMREVFEPHFELLYYSRGENLL
                     AEIDGLHPDIIILDVDMPGVNGFDVCRQIRSKKGLSAVPILFLTASRADQDYIKNRLV
                     GGTAYLTKPMMPDDLLAKIRQLL"
     gene            24290..25450
                     /locus_tag="P5079_01885"
     CDS             24290..25450
                     /locus_tag="P5079_01885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY28769.1"
                     /translation="MGRLGTETAFDVLVRARKLEAEGKSIVHLEIGEPDFDTPEHIVE
                     AAVKALKSGAHHYGPSAGLPQMREAIAKYVRDTRGVPVDPAEVVVTPGAKPIIFYTML
                     ALADPGDEVIYPNPGFPIYESMINYVGAKAVPVPLREDHNWSLDVEKLAKCITRKTKL
                     LILNSPHNPTGGMIPMSDLKKIAELVQKHGIIVLSDEIYSRVMYMDNYPSFYSIPGIR
                     EQTILLDGFSKIYAMTGWRLGYGVMPKDLAVQVSKLQTNSASCTATFTQLAGIEALTG
                     NQSLSEAMVAEFRRRRDLIVKGLNEIPGFSCKMPDGAFYVFPNVKKIGLPSKKLADMI
                     LEEAGVACLSGTAFGSWGEGYIRFSYANSQEKIQEALRRVKKLVVDRNLAKA"
     gene            25539..26312
                     /locus_tag="P5079_01890"
     CDS             25539..26312
                     /locus_tag="P5079_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY28770.1"
                     /translation="MKIALCQFDQVWEDREANKAKIKALVASTPKPFDWIVFPEMTLS
                     GFSMSLDKTTLTKDDSAFFSALARDRNAWVSFGGVQNGRNDLITLDRAGKLVDTYSKI
                     HLYSFGEENKFYKAGTKVSKFDLEGLRVVPTICFDLRFPYLFWDAAEQADVFINIASW
                     PARRSEHYTTLVRARAIENQCYALGVDRTGKDPLLEYSGNSMLFDPLGKVLLDCGTQD
                     GVFVSDIDVTRDLVTKTRTRFPFFRDKKAPGAYECLKKN"
     gene            26349..>26564
                     /locus_tag="P5079_01895"
     CDS             26349..>26564
                     /locus_tag="P5079_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28771.1"
                     /translation="MRLIGYGDSVRPGVYRRHSAFEKAVNFTDGERLVSLVAEDVGRG
                     PINLVFKDFNATLCRGPLKVRRTSLSVG"
BASE COUNT         5073 a         7415 c         8587 g         5489 t
ORIGIN      
        1 agctttgtcc gtggaggagt atcttcagga actgcagatg gaggtcgtcg tccgctcggt
       61 gggggaggaa acggcggcgc gggacgccca gttgacacag aagaccaatc agttcaacct
      121 gacgacgcgc cggtacacgg aatcggatat tcggtctttt tttgagtccc gggacacgtt
      181 cagcctcttc tgtctgcatc tgcgggaccg gttcggggat tccgggatcg tcggcgttgc
      241 cattctgcgc aaggagggcg acgacgcggc cacgatggac acgtttttgc tgagctgccg
      301 cgttttgggc cgaggggtcg agggcgcctt ccttttgatt tgcgcaaaat cctgtgtgcg
      361 tcggggttgc cgccgaatgt atgggttgta cgttccctcc gccaagaaca gccaagtgaa
      421 ggatttttat atccaaaagg gtttccggaa cgaaggaacg ggggaagacg gggcacagcg
      481 atatcttgcc gatgcggcgg agttgtctcg ccgggccgtt ccccgatatt ttaggtcggt
      541 gactctggac ggggaagagg tgaaataatg gaagaaaaac tgagaaaagt gatggccgcg
      601 gcgctggagg tgccggaggc ccgcatcggc ccggaggcca cgacggaaac catcgaaacg
      661 tgggattccg ttcagcatct caagcttgtt ttctcgctgg aggacgcgtt ccgggtgaaa
      721 ttcacgggga cggaaatcat cgaacttacc gggtacgggg cgatccggga acatcttttg
      781 aaaaaagggg cgaaatagac gatgagggga gcggaaaatg gccgttgagg tcctcagcag
      841 aaaaaatttt attgcggcgc ttcgccgggc gggtatccgg gaaggcgacg tcgtccatgt
      901 ccagtccgat ttgcggaccc ccgggccggt ggaggcttct ccggcggcga atcctcggga
      961 ggccatcctg gaattttatt tggcggggtt tcgggaggcg cttggcccga gcggcacgct
     1021 caccgtactg acgtcgtttg aggattacgg ccgctttgga acgcctttcg tcagggaaga
     1081 gtcgccgtcc cgggccggcg ccttcagcga atacatgcgc acccagccgg gagcggtccg
     1141 gtccgttcat ccgatcgtgt cgttgacggg gctcggagag cgcgccgagg agttgtgcgg
     1201 cgggaatcat tacgatggct gcggatacga ttctccttgg gggcggctgc accggctcaa
     1261 tgccaagttc gtctctctcg gtatcgggat gcggatcgcc ttctcgttcc agcactacat
     1321 cgagaaacag ttcggcatcc cctatcaata cacgaaactt tatcgcacgc cggtgtattc
     1381 gaacggtcgg gaagtgcccg ggccgttcac catgtccgtg cgctatttgg atttcggcat
     1441 cgcctatgat ttcagccggt ttgaaaaacg gctcttggaa aaaggggcgg ccgtggaggt
     1501 cgcgctgggc cgcgccatgt tgcaactgac gaccgctcaa ccggcgctgg acgtggggat
     1561 ggcctgcctg aacgaggacc ggtatttctt cctcaagacc cctccgacgt tccgggacgg
     1621 ggagtttccg ttggacggtt ttaccggacc ggaaaaatat atgtatgacc ggggaaaaaa
     1681 cgacgcgccg cgcgcggagg aaaagccaca tggttgattt aaaaaaactg gaccggctgc
     1741 ttcaactcaa catcggaatt tcccaggcgg ccaaccgcga gatgatggat attttcaaca
     1801 acgtgacccc catccgggtc cgacgctatc ccgcgggtag ggaatacaac ggctgggtgg
     1861 tgccgcacga ctgggtggtg aaaaaggccc atatcaggaa gaaagggaag attctctttg
     1921 acggcacggt ccatcccatg gcggtggcgg gatattcgtc gtcgttccac gggacggtgt
     1981 ccaaaaagga attggacaaa catctattca caaagaaaga gttcccggcc gcctatccgt
     2041 ttcattccat ctacaactac cgcccctggg ggaaacattg ggggttctgc gtcccctaca
     2101 acgtctgcaa aaaatggccg gccggcgact acgagatcga cttggagacg gaattcaaag
     2161 agggagagat gctggcgggc gaatgccgac accccgggga gctgaaggac accgtcgttt
     2221 tcaatgcgca tacctgtcat ccgtgccagg ccaacgacga catggcgggg gccctggtcg
     2281 tcctggaact gttcaaatgg ctggccacgc agaaaacgcg ctacagctat ctcggcgtcc
     2341 tggctccgga acacgtcggc acggtttttt atctttcgga tttttcggac aaggaagtgg
     2401 cggagttgaa gctcggatgt ttcgtggaaa tgcccggcac aaaaacacca ctggtgcttc
     2461 aaaaaagttt cgagggaaac accatcatgg accgcgtggc gaaatacgtt ttgggacagg
     2521 agcagccgag gctccgggtg ggagagttcc ggacggtggt ggggaacgat gagaccgtat
     2581 gggaggcgcc cggcatcgag gtccccatgg tttccatctc gcgttggccg tacaggcagt
     2641 accataccag cgacgacaac gtgaagatca tggatccgga aagactggaa caatttctgg
     2701 aatgcctgaa aagaatcgta agggtgttcg aggacgaccg gacgatagaa agacggttcc
     2761 gcgggctggt ctctttggcg aaccccaaat acaacctcta catcgagcgg cccgaccccg
     2821 tggtggacaa ggggctttcc ccgatggacc tgcggttcgg ccggatgcag gatccgctgc
     2881 cgcgttattt tgacgggaag cacacggttt ttgagatttc cgaaaaattc gatgtgccct
     2941 ttgagaaact ttcggcctat cttcgggaat tcgagcggaa agggttgatc aaactccaac
     3001 cgccgccgtc gctggaccac tataaaccgg ccggagcttg aagcggttgc tcgtccggcg
     3061 ggagagccga tgataaagtt atttttcttg gatcatccgg aacaggtttc ttgggtgatc
     3121 gaaaataaag aggcggtcgt ttcccccgga acgctggtct tggccatgag ttccagggtg
     3181 ggcgaacgct tgagacaggc gggggttccc taccgcattc cggaagaatt catcgcgccc
     3241 gccgaactta cccgacgagg catggagaat ttcaagtggc tggaaaggtt ctgccattac
     3301 agcgatcgat ggctggccgc ccacctggag cggatccgtc tccacgccct cacgcccacc
     3361 gcctggaatt attccgcatt aaagaacatc tttgatccct tttttttgag gacgctcgag
     3421 gtcaaggcga ttttggagtc gcaaagaccg gaggaggtcg tctatttcga ggtcccggcg
     3481 gtggcggccc tggacgatta tgtggacaat cgggagtcac tctccgcggc cgttctcccc
     3541 cgggtggccg accgtctgaa gatcgccaag ctgtcgccgc tcccggccgt cgaatcggcg
     3601 ccgtcgggag agggccggcg cgtcgatttg agagggtacg cgcggcgttt tttctgggcg
     3661 cgttgggcgc ggacgctgca gaaatattat ggggcggcgg ggttcctccc ggcggcgctg
     3721 tccgggttcc ggaagaaaga ctgcgtcctg gccctgaacg tggattacga tctgttcccc
     3781 ctatttcgcg aagccagcgc ccgccgaacg ttccgcgtct tgttctggga gccgggcagc
     3841 gaacacgctc cccggttcct gtgccctccc gctgtttttc cggcttttcg ggggaaacgt
     3901 cccgatcggc ggttcatgga ggagacaagt gttgattgga aacgcttgac cgacgataag
     3961 gagtatcgtt cgtttttcgt catagacggc gtagacattt ttccggcagt tgaaaagcaa
     4021 ctgaggcatt tttatgaaga tatcttaagg aggtcggtgt ggagtttttt cgaagcggaa
     4081 cgcgtgttgg acaagttccg gccgcgtgcc gtcgttacgg aatgtctctc ccgtccccag
     4141 gacgccgccg tttttgaggc cgcccgtcga cggcagatcc cgcgggtgat ctactcgcac
     4201 ggcccgtacg ggatgccgtc cactccggtg acggattatt tggactataa aaggaccgac
     4261 tattttttgg cgtgggggga ggggggacga aaggatgtgg aacggtaccc ggcgccgaac
     4321 ccccgcgtgg aagtggtggg atcgggggcg ctggacgctt tgaaggagcg gccgcggctc
     4381 gacgcgcggg gccgggcggc gttgtgccgc tcgctggggc tggagccggg ccgccggacg
     4441 gtgatccatg tgctcgggcc cataagcgag aacggatatc gcgtcggcgt tgatttcaac
     4501 gacagggatt atttcacttt cgacgtcgag aagaagatcg taaaggcgtt tcagaaattt
     4561 ccggaggctc agtttttgtt gaaacttttt ccgttccaag gcatgacgga tcccatcgtt
     4621 gatttcgccc ggcacgaagg gttccggaat ctgcgcttcg tgaagtggac ccccttcgtc
     4681 aagctgctcg acctggccga ccttgtggtg gtggaggttt actccacccc gttgatggag
     4741 gccttggcgg cgggaaagcc gattgtgatg ttcacgggac aggaatcccc ccttgaatcc
     4801 ttgggggatg tgtccgaggc ggtgcatttt gcggggaaca cggaccagct ggagaggctc
     4861 ctccgctcct ttttgcgagg ggaccccgcg gcgttcccgc ggaagaacgg cgaggctttt
     4921 ttgcggaaat acggaacgca tctcaacgac ggccgcagcg cgcagcgggc gctggaggcg
     4981 ttgaaaacga tcgctttggg gcaggagggc tagtccgcat gtcgttggcc gtcaacatca
     5041 ttacttatat gtccaccggg gtcgtcgttc gggccattac gttggtcacg aacctcgttt
     5101 tccggcgcat cctggggccc gagatggccg ggatctggaa tctggtatac atcgtcaccg
     5161 ggtatctctc gacggtcagc atcggcgtcc agacggggtc ggagagggac atcccctatt
     5221 ggcgaggtaa aaagaacgcc gagatggaac gccgcctcgt ccatcaaatg ttcaccgtga
     5281 gttttcttga ggcggcgatg gtggggagcg ttttccttat ctatttattt ttcttcggcg
     5341 gaaaatatcg gcacgacctc tacatcggtt tcctttccgc cgcgggttac gtggttctgt
     5401 ggcgcatgat gggatgtttc gtggtgggtt tccgctccat tcaaaatttc acgtttttgg
     5461 ggaagagcga ggtggttttc gccgtcctgg acctggggct gatcgtcggc ttgacctcct
     5521 tctatggact gtatggacag tatctggcgc acgccctcgg gttcctgttg aaactttcat
     5581 tttggtactt gtggacgcat aaaaggaaaa tgttcgacat cggttggcac ctggtgttgg
     5641 ccgatatcaa gccggtcgcg gccgtcgggt ttccctggct cctcagcagt ttcgtatggc
     5701 agttcatgat gtccgtggac agccttttgg tggccaagat ggcgggggtc accgcgctgg
     5761 gctactactc gctgggcgtg tccatcgtgc ggatgttgac ggaaatcccc aacacggtca
     5821 gcacggtgat cttcccgcgt ctgctggaaa aattcggcga acgagaggac atccggccca
     5881 tcaaagagga cgtggtccgg tacctgaaga accttgcgtt cgtcgtgatc ccgctccttt
     5941 tcccgctcgc ctatttcttt ttcccctggg tcgtccggca gttcctcccc aagttcctcc
     6001 cgtccatccc ggcggccaag gttttgctgc tggccgccct cttcatgccg atgatccaca
     6061 tgccgggcca gatactgatc ctgcagaaaa ggatatggct ttatgtcggg ctgcagttgg
     6121 gcgccgggat agcgacggct gtcctggtgc tgctgggggt ccgggggggc atcgtgatgg
     6181 tggcggccat ggcggtgttg tctcagttcc ttttcttttc cgccctgttc atggtttctt
     6241 tcgcgcgcgc cgacctcccc aaaaacgttt tttcgaatta cgcgggactg ttgctccccg
     6301 gcctctactg cggactgtgg gtgttcgggg tggatcagct tctgctccgg tggggggcct
     6361 tcgggacctg gttttcggat ttgctctcgt gcggcggcgg ggtcctgttg tcctatacag
     6421 gcatgtcggt tctgtttttg aaggcggaac gggaactggg gatatggcgg catgtcgtgg
     6481 acttcggcca gaagttgaaa acgttgtgaa ccggggagaa gagcgatgag gaaaaagatc
     6541 gcctacttcg tggacaccaa cgccctggga ggaggcgaaa aatacgtcgt cgacctgatg
     6601 gccgccatgg ggaaacgcgg gcacgaggtg cttttcctct gtcagcaaaa ggaggaactg
     6661 gcggcatacg tcgggcggat gtgccccggc gtgcggatcg tcgcggcgcg ctttccgagc
     6721 gtgtctcgga gcgccgccat ccaggcgggc ttggccatca accgccggtc gaataagatt
     6781 ttcgagtggc tgaagatccc cgggctggcc gtttattatt tgaacgtcct gtatggtttt
     6841 atcgtcctgt ggcggctgtt gcgtcggcac cggcccgagg tgctgcacgt cagcaacggc
     6901 ggttaccccg ccgccgaaag ccaccgggcc gcggtgttgg ccgcgcgatg ggtggggata
     6961 ccaaaacgcg tgatgacggt gcacaacatg gcatcgggct acccgcccgt ggcttgtatc
     7021 gagaaacgat tggatcggtg ggtggaggga tcgctgcacg ccatcatttc cgtgtcccaa
     7081 cgggcgaaag aggcgttggc ggtccagcga ggttttgata ccggccacgt ttccgtcatt
     7141 tataacggca ttgtccccca tcgcgtctcg gaggcggaag gactccggcg cgaattggga
     7201 attcctcccg gagtgaaagt cgtcggaaac atcggcggcc ttcagccgcg gaaagggcat
     7261 gaggccctgc tggaagcttt caaaaaagtg cacgcggaat ttccccggac cgtcctggtc
     7321 atcatcggtg aaggcccctg ccgttcggcg ttggaggcgc aggtccgggc ggcgggactg
     7381 gaaacggtcg tcctgttgcc tgggttccgg cccgacgccg gtcgttacgc ggacatgatg
     7441 gatgtttttg tttttccctc gctggcctat gaaagtttcc cttacgtcgt tttggaggcc
     7501 atgtcggccg gccggccgat cgtggcgacc gacgtgggtg gcacggcgga acagattgtc
     7561 gacggtcagt gcggccggct ggtcccggcg ggggatgttt ctgaaatgag ccgggcgatc
     7621 gggcgggtgt tgtcggatga aaacacggcc gcttcctggg gcgtggcggc gaagaaaagg
     7681 gtcgaggagc tgttcacgct ggagggcatg atcgaaaaga cggagaaatt gtatttatga
     7741 atcgtcggcc ggtatttcgg gagcttgaag agggaacggc gagatgaacc gatatcgcgg
     7801 gaaagaactt tttccttatt tgcggcatgt ggccggacgg gggacgttcg tggcggcttt
     7861 ccgggcgggc tgggaggcgg ttgcccgtcg ccgggggtcg ccctgcgcgc ccccatggat
     7921 ggcgttcccg ccaaaggagg cggacgttac tgtttcggct tcgggcgttc aagcggcgat
     7981 ttgggggccg ttgtggccga tccatgccgg tgtgccgctg ccggaaccgc tttctgtccc
     8041 gttggcgacg aagaatcacg acctcgggga ccgggacctc tggcggacgg aattcgaaga
     8101 catagaagac accttctccc tccatcggtt cggttgggtc cccgtgtgcc tcgggcggca
     8161 tggaacgatc ccgttgggga tggtcctgtg gcagcgggtc gttcaatgga tacgggaagt
     8221 aacgccgtcg cagttcccgt cggcgtggga gacctattcc gcctgcgagc gcatcgtcca
     8281 ttggagccag ctctacgcgt ggcttcgtcg cgcggcgggg cgggggctgc ccgaagagga
     8341 ggcgttcgtt gaaagtctgc tgctccacgg tgaatatgtc ctatcccatc tggaatttta
     8401 cggagaggac gattcgtcga aaacgaacaa ccatctgatc aacaacggcc gggcgcttta
     8461 tgtgctgggc gcgttcttga acgttccgcg gtatctcgac accggccgcc ggctgttggc
     8521 cgacggcatg cggcggtctt tcacccaggc cggtttcttg cgtgaagggt cctcccatta
     8581 ccacatcttg ctggtgtcga gatatctgga tgtcttggag gtcgcgagga acatccggga
     8641 cgaggcgttc gtcgaggaac tgggcccgtt tttggataga agcatggccg cggcgcgttt
     8701 cttccttccc gagaaggagg gggtcttccc gctcatcggg gacgtgtctc cggatttcga
     8761 tccggaatgg accctccgtg ccgtggaaga tgcggcgcgc cggttgttgg gggcgggcgg
     8821 ggggctttat gggaacgttg cttctgaagg gaagggggtc acggaccgcc tccgggacgg
     8881 atggtgtcga tacgacgatc cgaattaccg cgtctactgg catgttcagc cgggcggcgc
     8941 catccccccg tattcccacg cgcatcacga cacgggttct tttgaattgt ccgtggaacg
     9001 acatcccctc ctggtggaca cggggcgtgt cagttatgtg gccgacgccg ccggattgta
     9061 tggtcggagc gccgccgccc acaacggggt cctcgtggac gggcgtgaac cgttcgtgtc
     9121 gcattggctc aacggcggac ggttcctgat gagcgactac ggtcggaccg aagtgcggac
     9181 gtggtgggag aacgcctcgt cccgccccgc gttcgtcgtc gaacatcagg ggttttcccg
     9241 gctcaggggg attggaaagt atcgtcggcg attcttgttg gataaggatg aaatcgtcct
     9301 ggaggacaga tacgagggcc gcgggcggca tgttttggag acgttcttcc atgccgcgcc
     9361 ttcggttggc gtcgcggtcg aagggaacgc gctgtcgttc cgggagaagg ggacgcggcg
     9421 gaatctggaa ttggccctcg acgccggaaa ccttgccgga ctgagcctga tgcggggaga
     9481 gtgggaaggc ggccggccga tgggctggtt ttctccgcgt tacggacgca atgtccccac
     9541 cacgacggtg cgatttcggc acaaagcggt gttcccattt acaaatcgat tcgttctccg
     9601 gctggggtga accatgtgcg gcatcgcggg catctttcat aaggaagggg atggggcgga
     9661 ccggaatgcg gtccgtgcca tgtgccgggc actgacccat cggggtccgg acgacgaggg
     9721 atttttcgac agccccggac tctccctggg gatgcgccgg ctcagcatca tcgatctcaa
     9781 gggcggccgc cagccgttgt tcaacgagga cggttcgctg gttttggttt tcaacgggga
     9841 ggtctacaac tacatcgaat tgcggcggga acttctctcc cgcggacaca cgttcgccac
     9901 ccagagcgat ggagaggtca tccttcatct ttttgaagag aaggggctgg acgctttgca
     9961 ggaactgaac gggatgtacg ccttctgtct ttgggacgtt cgtaaaaaag agggatatct
    10021 cgtccgggac agattgggga tcaagccgct ctattaccac gtgacgccgg agagccgccg
    10081 gctctgcttc gcctccgaaa tcaaggcgct cctccacgcc cccgtggaaa aagaagtgga
    10141 catggaggag ctcttccggt atttggcgta catgtacatc cccggttgcg ggacgccgtt
    10201 caagaacgtc cgcaagcttc aggcggggag tttccttcgg ttcggcgccg aggggatcgg
    10261 cgcgccccgg cgatactgga cgatgccggc cggggacccg gtggaagacg aagggcggct
    10321 gaaggaggag tttttctccc ttttggagag ctccatcgag ttgcaatcgc ggagcgatgt
    10381 gcccgtcggc gtttttttga gcggcggctt ggactcgtcc ctgatcaccg cctacttttc
    10441 gaagaagacg aagtcccggg tcctttcctt caatgtcaaa tacgaagggg ctccgttcga
    10501 tgagagccgc tacgcggaac tcgtggcgga aaaatttgga tgcgaacaca agtcggtgaa
    10561 cgtgaccccg gacgacatgc tgaaggaact gccgcggctg gtctggcata tggacgagcc
    10621 ccacgccgac catgccatgg tggcggccta caaggtctcc gagctggccg ccaaagacgt
    10681 gaaggtggta ctcaacggca cgggcggcga cgaacttctc gggggatatc cccgctatcg
    10741 gcccaatgcc tggccgctgg ccgcctggca caagtcgccg gaattcctga ggacgctggc
    10801 cgtcgggatg gcggaaagga ttccgttcgg cggcgacttc gttaaaaaag cgcggttcaa
    10861 cggcacgccg gacgatacgt acgtctggag actccggcag ttcaagccgt atcacctgga
    10921 acggttctgc gcagaccggc ccgttgccta cacgcgcgat gcgcgggtga gggaatggct
    10981 cgaccgcgcc ccgaccggcg atctgaaccg ctacctgtat ctcgatgcca atttctatat
    11041 ggtggacgac ctcctcctgc ttttggacaa gatgaccatg gccgcttcca tcgaggggcg
    11101 ggttcccttg ctggaccacc gtttggtgga atgggcgttc aaaatgagcg gccggtggaa
    11161 agtgcgcggc ggtgaaacga aactggccgc caagcggtgg ttgcggggca tcctcccgga
    11221 ggagatcctg tggcggccca aactggggtt cggcgcgccg atgcgcgagt ggatgaagga
    11281 caaactgttt cccaaggccg ccgggttggt ggccgcccgg ccgcccgcgc gggaaaacct
    11341 cttttggggc ctacgggggg aggctttgcg cgaaaggatg cggcgactca acttccaaca
    11401 gaactatgcg cttctggcgc tggaggtctg gttccggctt tacatcgacg gcgaatccat
    11461 cgccgaagtg acggaacgga tccgaaaatc atgaagaccg tgttgatgct cgtctatttt
    11521 ttcccgcctt cctcgggcgc cgccgcccag cggtcccgca agctggccaa atacctgccg
    11581 aaacacggct ggcagccgct cgtggtgacg gccggggacg atccgttcgt ccctcgcgac
    11641 aacacgttga tggaggacgt cgcgtcgacg gaaattcatc gaaccctgcc cagcattttg
    11701 cccggcatgc ttttgggccg tcgggccctg cggctcctgc tggatcgttt ttttgttccg
    11761 gatatccaag tcggctggat cccccgagcg cattcctgcg taaaaaagct gctcgcgggc
    11821 cggaagatag acctcgtcta tacctcctgc gcgccgttca gcgccaatct ggaaggggag
    11881 cggatcggaa aaagatacgg cgtgccgtgg gtgacggatt ttcgggatcc ttgggtggcg
    11941 ggaaccacgt tccgtccgcc cacgcccgcg catcgctgga tccatgcggc gctcgaggac
    12001 cgcgcttttt cccgctgcga ttggttcgtc gccaacacgc ccggttttct cgacgaggaa
    12061 cgggagcggc atccggtgtt gcgccagaag agctgctgca tcagcaacgg ctacgacccg
    12121 gaggattttc cgtccgtccg ccccgccggc ggcaaagaaa cgtgggaaat ggtttttacc
    12181 gggtcctggc accggcggta ttatccggac acgcttttcc ggatcgtgag gttttttctg
    12241 gagaggaacc ggaccgcgaa gataaagatc cattattccg gtccgcactt caaacagttt
    12301 ttcgttccgg ccgaacggaa cggtctggcg ccgttgttgg aggaccacgg acatttgtcc
    12361 tataagagga cgatcgcgct tttgcagtcc ggggacctcc tgttgaccac attgccggac
    12421 gacgcgcgcg cccggacgtg ggtccccgcc aaactttacg aatatctggc cacgggccgc
    12481 gcggttttcg gcgtctgtcc cgacggcgac gcggcccggt tggtggagtt ggccgggtct
    12541 ttcgtgcggc cgaacgcgct cgacgccgaa acgcggggcg gcgacatcct gcaggggttg
    12601 tgggacgatt ggaaggcggg aaaacggcgc gagacggcgc gcgccgtctt ggaaaaatat
    12661 caataccctg tcctggccga acgtctcgcc ggcgtgtttg accggttgac ggagaaaatg
    12721 ggatgagccg gaaaaaagtt ctccacctga cggtcgggtt ggggctgggg ggggccgaga
    12781 aattgatggc cgagatggcg gcgcgacttg atccgtcccg ttacgaaacg caggttttgg
    12841 ccctgaaagg ggagggcccc ctgggggatt cattgcgcgc ccgcggggtc aaggtgtccg
    12901 ctctcggcgg ccggggcccg tgggacgtgc gggtgctgcc gcgggccgcc cgattccttc
    12961 gcgaggtccg ttgcgacatc gttcattccc atctcttgtg ggcgaacctg ctggcctgtc
    13021 ttctgaaagg gcgcagcaaa gtcgtctggc acgaacacga catggacgat tggatgggac
    13081 ggccgcagaa aacggcggaa cgcctcctca tcggccgggc ggacacggtc atcgccattt
    13141 ctcgggcggt ggcggactgc gtcgcgcgaa gagagccgtt cgtgcgggac aggatacgga
    13201 tattgcataa cgccatcccg ctgaaggaga agccgacatc gccgcagggg aaacgaagtt
    13261 tccgcgcggc cctcggattc ttcggggagg aaaaagtcgt gggctacgtg ggccggctcg
    13321 acgaacccaa aaaggggctg aaagtgctct tgcacgccgc ggccgagatc gccgccgagg
    13381 acccgtcggt gcgatttgtc gtcgtggggg acgggaacgc gcgggcggcg ttggaggcgg
    13441 aggccgaccg cctggggctg tcgaacagcg tccgtttttg gaaggcgcgg gaaaacgtgg
    13501 aggaagtcta cggcgccctg gatgttttcg tcctcccctc cctttgggag ggcttcggca
    13561 tctccttgct ggaggctatg gatgaaggat tgcccgtggc ggggtcccgc gtggggggaa
    13621 tcccggaagt ggtggtgggc ggcgaaacgg ggctcctgtc gccgccgggc gacgtggagg
    13681 cgttggcggt gaacctgagg aagcttttgg ccgatccggc tttgcgccgg cgattgggct
    13741 ctgccggccg cgcacgggta caggcccagt tcggtatttc gaaaatggtc gaggagttgg
    13801 aaaaaattta cgacgaaacg gagaaaaccc aatgaacgaa acggccctga aaaaatggag
    13861 ccggtttcac gcgcccatgg gctgttcggt cggtggcgtg gacggccggg tggaggcatt
    13921 gctgcgcggc cgggtgttga acatcggcgc caaggcctgg ggccggccgg aatggattcc
    13981 tctggacctg gtgtcgaacg acccccgcac cgcccttctg gccgacgcgg cggatttgcc
    14041 ggtgaggagc ggtgttttgg acggggcggt gctgcgtttc gttttggaac atgtgccgtc
    14101 cgctgagcgg gtggtggctg agacgcaccg ggctctgcgg ccgggcgggt tactcttcgt
    14161 cacggtgccg ttcgtggagc cgtaccacgc ggacccgcag gactttcggc ggtttacgcc
    14221 ggaggggctg aagaaattgt tggagaattt cgagatgttg gaatgcggaa cctactacgg
    14281 acccgcctcg gcgttggtgg agttcctgcg ggagttcacc gcgtcatttt tcgactcggt
    14341 ttttctgaaa aaaggggttc gttttctcgc gggctggacg tttttcccgt tgaaatatct
    14401 cgacgttttc ttgggacgca agaaattttc gtggatgtgc gcctacagcc tctatgccgt
    14461 ggcccaaaaa cgttgagcgg gcaccggcgg cgggtcctcg acagagggga acaataacag
    14521 tatcctttag accggataag gcatgaaaat cctactcatc aatcccatca cgcggctcaa
    14581 caattacgtc tccaatcttc cgctgggcat cgcctatgtg gcggcctccc tgcggcagga
    14641 ggggcacgat gtccgtatct tcgacctgga cgcgcacaaa tacggcaagg aggacgtgcg
    14701 caagacgctg gagaagtgcg actacgacgt tttggggatg accggcatca tcacttcctt
    14761 tcagcagcag cggtggatca tcgacgaggc caaacgggtc aacccggcca ccccggtgat
    14821 cctcggcggt gtcggggtct cctccatccc tgaattgttt ttccagcaga ccaaagtcga
    14881 cgtggtggtt tgcggcgagg gagacattgc catccgcgag gttttggccc ggttgagcga
    14941 caaaaaaggc ctggccgatg tgccgggcat ccattatcgc cagggcggcg gcgtggtgaa
    15001 aaatccgccc cggtccgtgg tgcgcgatcc ggacaccctc ccgttccccg cctgggacct
    15061 cctgccgatt gaaaaatatc tgtgtcggcc cagccccgcc tggcacaaaa agaacatgac
    15121 gtttctcacc gcccgcgggt gcccgtacca gtgcaccttc tgccagccga ccggcgggca
    15181 tctctaccgg taccgcagca tcgacaacgt catcgcagag atgaagaagg ccatgggact
    15241 ctatcactac gggcacgcgc tcctttccga cgagtgctgg cccatccggc gcgaccggtg
    15301 tttcgagttc tgcgagaagg tgaagccgct gaacatcgaa tggagctgca ccggccgggc
    15361 gaacctcatg gacgaaccgc tggtcgaggc catggccgat tccggctgcg tgatggtggg
    15421 aatggggtac gagtcggcca gccacatcat cctcaagaac atcaaaaaat cgcagacggc
    15481 cgaacacgaa cgcaaggcca ttcggcttct gcagaaatac ggcatcgtgc cgtccaccag
    15541 ctacatcgcc ggactgccgg gggaaacccc ggccactctc cgggaaacgg tggatttcat
    15601 cgaggatctg gacgtcccca tcttcgattt cttcttcgcc acgccgtacc ccggcaccgc
    15661 cctgtacgac tacgccgtga cccgcggaaa aatcaccgac ccgctgccgt tggtggaatc
    15721 ctacgaggag atggcttcga acctgctggt gaacgtgacc gacatgagcg acgaggagct
    15781 gatccgcatc aaacgggaag ccgaggtgga gttacgcgaa gtctatcgtc ggcggcatcc
    15841 gctctatttc catttggaac ggctcaaggg attttatgag cggttgctcg cccgctggcg
    15901 cagcgggtcc ctgccgaagg tctttctgca ccggctggga cgctttttga ggaaatgggg
    15961 gattgcggat tcggaccggc ttctgcggag cggcgaatac agctggcttt cggacaagag
    16021 agtatgaacc aaccaaagga agaaccgaag gaaggcgcgt cggccgccgg gtcggccgtt
    16081 tttataaagg aagcggcggc gtgggaccgg ttcgtctccg gttccgagaa accgcacatc
    16141 atgcagtcct gggcctgggc ggaactgaaa gcgaagagcg gatggcgtcc acaccgcctc
    16201 ctgatcgagc gacggggggg cgcgcgggtg ggcgtttccg ttttggagcg ggatttcccc
    16261 aagatcggcc gacggttttt ttacgcgccc cgcggaccgg tggtggccac cggctggcga
    16321 ccggaagatt tggagcaatt gttcctcgaa ctggcagggc tggccaaaaa ccgccgcgcg
    16381 gtgttcctga agatggaccc ggacgtttct ttggaacagg actggatatc gtcggacctg
    16441 cgcggcgccg ggtttgtccc ggccaccggc gggggcgggt tcgccggcgt gcagccgagg
    16501 tgcgtgttcc gtctgaagat cggcgcgtcg gaagaggagc tcctgaaaaa catggaccag
    16561 aagacgcgct acaacatccg gctcgccggg aagaaaggag tgaccgtgcg gccggtgagc
    16621 gaccgggccg aaatcgagac cttccaccgg atactcatcg agaccgggca acgggacggt
    16681 ttcatggtga ggcctttgag atatttcaac gatatacagg atttgatggg tccgcgcggg
    16741 caggcccaat attttctggc cgaactcggc ggcaaggctt tggccggcgc cctggcgctt
    16801 cggttcggtc ccgtgtgctg gtacgcctac ggggcctcgt cgaacgaggg gcggcagcac
    16861 atgcccaacc acctcatgca atggaccatg atccgttggg cgaaaagcca gggctgcggg
    16921 ctttacgatt tccgcgccgt gccggacgac cccaatcccc aaaaccccct ctatgggctt
    16981 taccggttca agaaggggtt cggcggcgac ctggtccgtt ttgtgggcga gttcgacaag
    17041 gtgtattcgc cgtggtggta tcggatgtgg gcatacggct ggccggtgta taaaagtgtg
    17101 agaaaattta tgcgcggccc ggcgcggaag gcggggagaa acgaatgaaa cctttacgct
    17161 gggtggaagt ggatctgaac gcggtggggc acaacgtgaa atccgtgcgc cgacgcatcg
    17221 gaaagaaaac ggggtttacc gccgtcgtga agacggacgc ctacggacac ggcgcggttc
    17281 cggtggcgcg cgcggcgttg cgttgcggtg cggacgggct ggcggtcacg tatctggaag
    17341 aggcgcttcc tctgcgacgg gcgggaataa aggcgccggt gctggtgatg gggccggtct
    17401 ctccggccgc ggccccgttg gtccggcgct ggaacctgtc cgtcatggtg gacaacgaac
    17461 cgctgttgaa ggcgctcaaa cgggcgggga ccgcttccca tcccatcggg gtgcaggtga
    17521 aggtcaatct gggtttgtgg cgatggggga ttccactgcc gagattggga tttttttgca
    17581 gggaagtaga cgccgccccc tccctgcgtc tggaagggat ttttgcccat cccggttata
    17641 tgatcggaaa aaacaaatcg cgcatcgagg accaactgcg gaacttcgtc gcggcggcgg
    17701 acgcctgcaa aaacgaattg agacggtctc gcccggaact ccacgccgcc gacagcgccg
    17761 ttctgctcga tttcccggaa ttccagctgg accgcgtgcg cgtggggaac ttgatctacg
    17821 gcatcaatcc caccgacaag ccgttggagc tgagaaaccc ctggcgggcc ctgtcgcgcc
    17881 tcgtccgtct ggaacggctt gccgtggggg acatggtggg ctatgggggg gaattcgtcg
    17941 cgcctcaggc catgaccatc gccaccgtcc cggtcggcta cggacatggg ttgaccctgg
    18001 aacccgccag ccgctggatc caactgaaaa gcggacagaa ctactgggga aagatccgcg
    18061 gcatcgtctg tccgttcgtc ggtcgcgtgg ggatgagcca ctgtctcgtg gacgtgagtt
    18121 ccgtgcccgc gccgaagatc ggcgacgtca tccagctgcc tctgcggcga accgcctcgc
    18181 tcggttggcc gaaaatctat tcgtctcaat aacaccgccc ggtccgatac acgcttcccg
    18241 ccgcgtattc tcccgtgatt ccgccactca acatcttctc gccccgcgcc ctgttccgca
    18301 agggccgcat cgaaaggccc tgaacaatgt atgtcgtggc gctgcgccgg gtccgtcgtt
    18361 ttattgcatg gggaagcaaa gcggttttaa aaacactgct cgtcctggtt attgcggaag
    18421 gtgttctgtg ggccaccgga catctgtatt tgtggtatta cacgaaattc gcctggaagc
    18481 ccgcggatct atctcccgga atccgccgaa tactgttcat cggagaatcc accgtggagg
    18541 ggcagcgttg cgacgagggg gattttaagg ggccgttccc ggcgcagttg gaggcgatgc
    18601 tgaaccggat gtcggggcgg aatgaatttt cctgcatcaa tcacggcatt tccggtgtca
    18661 ccagcgcgca catgctccgg tatataggaa aggatctcgt ggattacgcc cctgagatcg
    18721 tcgtccttca ggcgggcatg aacatgaaca tgcccagtat ttttaggctg atccggaggg
    18781 cgggatggga catcgaggtc gacagcgtcg tgctggcgaa acttcaccgg ctgcgcatcg
    18841 tccgtttcgg gaaactcttc tatgaaatgg ggcgcgccgg ggatttggac cccatccact
    18901 actatgagga cctcgaactg gagaacgccg aaaacaaggc caacatccaa acggtgattg
    18961 ataaaataaa aaaatacggc gcccgcgtgg tgatatgcaa ttatttcaag gagggatacg
    19021 ccaacgacat cctcaaaggc gtggtgcggg agaacggact gatcctgtgc gacaacgccg
    19081 ccgtttttaa ggaatggcag cggcgcggcg tcgaccggac gatcatttcg agcgacggct
    19141 ggcatccgaa cggcaacggc taccgcctca tcgcctgcaa cctgctcaag acgtttctgg
    19201 aaaaagacct cgtcccttac gccgtggagc cgtcaaaggc cgagcggata aagaacctgg
    19261 aagccgtttt ggaggggaac ctcgccgccg aaatttgaaa gggggagggc gaagggggca
    19321 ataagaacaa aagcccgaac gggccctatt ccacgatttc ggtttcggcg gaaatgagcg
    19381 gcaggagttt ttcgtggttc acgtcgcaga aattctgcgg cacctggttc tgccggaacg
    19441 cctccagcgc cacgtgcggg caggagggca gggccaggag ccccgtctcc gcgtcgatct
    19501 tcgcgaacac cacgccgggc ggaaccggga aatcccgcgc ggggatgtac ttcatcgatt
    19561 ccagcatgaa atccgtccac cacggcaccg ccgttccggc gctgctgatg cctttgccga
    19621 gaggggtgaa gtcgtcgtac ccgatccagc atcccgcggc caagtccggc aggtagccca
    19681 caaaccacgc gtcgcgcatc tcctgtgtcg tccccgtttt tccggcggcg ggccgtccga
    19741 gccgtcgggc gtaacgggcc gttccctcct ccaccacggc gtgcagcaat cgcgtgagga
    19801 gatagttgga ttgaggcgag accgcgacat tcccctggat gacgtgctcc tccaggagtt
    19861 ttccgccgtt gtcgagcact ttctggacgg cgaagggctc catgtggacg ccgttgttgg
    19921 cgaaggtggc gaaggagccc accagttcca tgggcgtgac caccgaactg cccaggccca
    19981 gcgagaggac ggcgtcgagc ggactccgga tgccggcgtt gtgggcgaaa cggaccacgg
    20041 tgcgcggccc cacccggtcg atgaggcgca ccgacacgag gtttcgggag agcgcgaggg
    20101 ccttgcgcag ggtgacgggg cccagatatt tgtcgtccca gtttttcggc gcccagacgg
    20161 ggtcgggggc gtcggggtcc atgtcgggcg ggtaataacc ggtcaccatc tcccgaagca
    20221 ccgcgtagtc cgtggattcg gccaccagtt tcggctgccg ttccacgtcg gtgaaggcca
    20281 gcggatagtc ctcgatcacc gtggcggcgg tgaagccgga ttccagggcc gccagccaga
    20341 cgaaaggctt gaaggtggat cccggctggc gcctggcctg ggccgcccgg ttgaactgcg
    20401 atttctggaa atcgcgtccg ccgaccaggg cgcggatgcc gccggtgcgg ggatcgatgg
    20461 cgatgagcgc cccttgaacg ttgaccggct cggcgttttc gtccgggact tccgtttctt
    20521 ccccggcggc ctcgcgctcc tttcgttttt tccagtcgat gtagtaactt tccgggatct
    20581 tcttgttctt caccagatag agtaggcgct gctcggcata acgggcatcg aaggaggaga
    20641 ggtggaactc ggaggtctct tcggcggctt tttgcatgcg caggtcgagg gtggtgtaga
    20701 tggagaaccc ctgccggtag agggaggtgg agccgtattt cggttccagt tggagccgga
    20761 cgtattccac gaaatacggc gccatggccg gggcgagagg tgttttcagg gtgttgagcg
    20821 gcgctccaca ggcctgcagc tcctcttttt cgctgatgta gccggcttcc cgcatgcggt
    20881 cgaggacgag ttgccgccgt ttgatggctc tcgccggatg tttgaagggg gaataggcgt
    20941 ggggagcttt cggcaatccg gccaggaggg cgcattccgg cagggtgagg tcggccacgt
    21001 ttttgccgaa gaacacccgc gccgccgcgt ccaccccgta agcgccgtgc ccgaaataga
    21061 tctggttcag atagagttcg aggatctcgt ccttactgaa gtgccgttcc atctccaggg
    21121 tgaggaggag ttccttgatc ttgcggttca gggttcgggc gtggctcagg aaaatgttct
    21181 tggccagctg ctgggtgatg gtggagccgc cctgggcggt gcggccggcg cgcaggttcg
    21241 ccaggaaggc gcgcagaatg cctttgatgt ccacgcccca gtggtcgtaa aaattccggt
    21301 cttcgatcgc cagcaccgcg cgctggaaat ccagagggat gttcggcaag gggaccacgg
    21361 tgcggcgttc gacgtacagt tcggagacga gttcgttgcg cacgtcgtag atgcgggtga
    21421 tgagcgacgg ggtgtaactc tccaggctgc cgatgggagg cagttgggcc tgataaaaac
    21481 ggaacgtcag cgccgcccat gtcatcagaa gacaatagaa cgtgagcagg aggagggtga
    21541 cgcggacctt gtgtgagagg cggcggaaag tggccaacat tccatccatg ctatttattt
    21601 ccagccgacg catcaagtcc tcattttaca tccggatcaa attgactata atgaatttta
    21661 tggcattttt aaattttcgg cgaattgcaa aagaacgcag caccgcgttg gttatgcgat
    21721 tgactctgtt cctggcggtc ttccttttta tcgcttacgg gcggtattcc catcgattgc
    21781 cgccgctcct ccttcttgga cttcttggac tcttcctttt tctggcaggt ctccaatttt
    21841 tcgtttcgga gaggtttttc cggtcccact atttccaggc cgccgttttc ctgtcagaag
    21901 tcacgttgtt gtctgtttcc atttatctga cccagggatt cagcagcgat ctgtacttgg
    21961 tgtacttttt aaccatcctg atggccggtt tttcgcgtca ggtgtggcag gtgatggtgg
    22021 tggcggtggc ttccagcgcg ttttacgggt tcaccctgtt tgtcgccaat ccggagatgc
    22081 ccctgtggga tcccggcctt ctgctgagga tccccttttt gatgttggtg gcggtcttcg
    22141 tctcttttta ttccatgacg gtacagatga aatccgttca gcttgaaaaa acgacaaagg
    22201 agcttaccca ggtgaaggag cagctgacca tggcggagaa actgtccgtc atggggcagg
    22261 tgatttcggg cgtggcgcac gagctcaata acccgctcac cgggatcatg gggttcagtc
    22321 agctgttgct gcgagaagaa gcggtggaaa acgattccaa actgcgttgg cagatcgaga
    22381 atatctttaa ggagtccgaa cgctgtcaga agattgtcaa aaacctttcc acgttcgcac
    22441 ggcagcataa accggaaaaa acccatatcg gcctcaatgg gatcctggag gattgcctgc
    22501 ggctggaggc ctaccagctc gtgacccgca atatccaggt gaagaaggac ctgttcccgg
    22561 aactcccaaa gaccatggcg gattttcacc agctgcagca ggtttttttg aatctcatca
    22621 tcaacgcgca acaggcgata ggggaatccc gggggaaggg gaccatcacc gtctccagcc
    22681 atcaggtcgg aaatatgatt cgcatcgaag tggaagacga tgggcccggg atcccatcgg
    22741 aaaacctggc gaagatattt gaaccatttt tcaccaccaa agaacccggc aaggggacgg
    22801 gattggggtt gtcgctttcc tacggcattg tcaaggagca ccatggcaac atttgggcgg
    22861 agaatgtgtc gtcgggcagg ggggcccgtt tcgttgtgga acttcccatc ctggaaggaa
    22921 gcgcaccttc ccattcaggg gaggggcggg tcgccgcggc ccctccggcc gccccggcca
    22981 ccggcaaaag gatactggtc atagacgacg aacagatcat cctggagctg ttccaccggt
    23041 ttctccgcca tttgggccac gaagtggatg tggcccgcga tggcgatttg gccatgcaga
    23101 aactccggag caatcggtac gattcggtgt tttgcgatat ccgattgcct ggcctcgacg
    23161 ggatcaggtt gttccggtgg gtccgcgaga accggccgga ttacgtgccc acgtggattt
    23221 tcatcaccgg ctccgtcacg tcggcgatgg agggggcgct caaagagagc agtcggcccg
    23281 tgctgagcaa gccgttcacc ctccaggcca tcgagaccgc cctgagggag accctgaaag
    23341 tatcttccta gcgttcctgc ccgccccaac gtcgtttctt ccgaaaacgc attggccgat
    23401 agaccttgga aaaacgatcc gccgggcctt ccgcaacggg gtcgccgggc ggttgtccgg
    23461 ttctggaatg acgggtttgg ggagagaata agattcttca ggccctcgga acgggtttcc
    23521 gtgccttttt tggggaactt ggacaggtct tccggtgttt tatatattca aggatattct
    23581 tgggatggga ccgaggtgag cgcgtggcgg acgagaagcc caaaaagccc cgggtggtgt
    23641 gggcggacga tgatttgagc ctgcagatcg tgatgcggga ggtgttcgaa ccgcatttcg
    23701 agctgcttta ttattcgcgc ggggagaacc ttctggcgga gatcgacggc cttcacccgg
    23761 acatcatcat cctggacgtc gacatgccgg gggtcaacgg gttcgacgtg tgccggcaga
    23821 tccgttccaa aaaagggctc tcagccgtgc ccatcctgtt cctcacggcc tcccgggccg
    23881 atcaggacta catcaagaat cgcctggtcg gcggcaccgc ctatctcacc aaacccatga
    23941 tgccggacga cctcctcgcc aagatccgcc aattgttgta aacggcgcgg ttttcccccc
    24001 acaattttta cgggacccga ttgcgcgggt cacgggaaaa gactatactt tagtccttat
    24061 ctgattttac gggcgtaatc gcctcagcga caaaacctcg aaataagaaa tgcagtctcc
    24121 cccgacacat ggcatcggag cgcggccatg gaagacgatt ttatttcttg aaaactgacc
    24181 gaacgacaag ggggcaccat tgaaagcgac tggcgtgaaa caacagaaga aaacagagtc
    24241 tggggcggcc ggcaagggac agggcatcgc atcgaaactt tccgtgagga tgggaaggct
    24301 gggaacggag accgcgttcg acgttctggt gcgggcgcgc aagctggagg ccgaagggaa
    24361 aagcatcgtc catttggaga ttggtgaacc ggatttcgat acgccggagc acatcgtcga
    24421 ggcggcggtg aaggccctga agagcggggc gcaccattac ggaccctccg ccggactgcc
    24481 gcagatgcgg gaagccatcg ccaaatacgt gcgcgacacc cgcggcgttc ccgtggaccc
    24541 ggccgaggtc gtcgtcacgc cgggcgccaa acccatcatt ttctatacca tgctggcgct
    24601 ggccgacccg ggcgacgagg tcatctatcc caatcccggg ttccccatct acgagtccat
    24661 gatcaattac gtcggcgcca aggcggtccc cgtgcccctg cgcgaggacc acaactggtc
    24721 gctggacgtg gagaaacttg ccaaatgcat cacccgcaag accaagctgc tcatcctgaa
    24781 ctctccccac aaccccaccg gcggcatgat ccccatgagc gacctcaaga agatcgccga
    24841 actcgtccag aagcacggca tcatcgtgct ttccgacgaa atctacagcc gcgtgatgta
    24901 catggacaat tatccgtcct tctattccat cccggggata agggaacaaa ccatcctgct
    24961 ggacgggttc tccaagatct acgccatgac gggctggcgg ctggggtacg gcgtcatgcc
    25021 gaaagacctg gccgtccagg tcagcaaact ccagaccaac agcgcttcct gcaccgccac
    25081 gttcacccag ttggccggca tcgaggcgtt gaccggcaac cagagtctct cggaggccat
    25141 ggtggcggag ttccgccgcc gccgcgacct catcgtcaag gggctcaatg agatccccgg
    25201 cttcagctgc aagatgccgg acggagcctt ctacgtgttc ccgaacgtga agaaaatcgg
    25261 cctgccgtcg aaaaaactcg ccgacatgat tctcgaagag gcgggcgtcg cctgtctctc
    25321 gggcacggcg ttcggttcct ggggcgaagg ctacatccgc ttttcctacg ccaactccca
    25381 ggaaaagatc caggaggcgc tccgccgcgt caagaagctg gtggtggacc gcaacctggc
    25441 caaggcctaa actaccgtac cggatccggg ggccctcccc cattgtcgct cggccgcttc
    25501 caagcggtgc ggccgtcgtt ttctccaagg aggacatcgt gaagatcgcc ctgtgtcagt
    25561 tcgatcaggt ttgggaagat cgcgaagcca acaaggccaa gatcaaggcg ctcgtcgcct
    25621 cgacccccaa gcctttcgat tggatcgtct ttccggaaat gaccctcagc ggcttttcca
    25681 tgtcgctgga caagaccacc ctcaccaagg acgattcggc ttttttctcc gcgctggccc
    25741 gcgaccgcaa cgcctgggtc tctttcggcg gcgtgcagaa cgggcgcaac gacctcatca
    25801 cgctcgaccg cgcgggcaag ctcgtcgata cctactccaa aatacacctc tactccttcg
    25861 gcgaggagaa taagttctat aaagccggca cgaaggtgag caagttcgac ctggaaggcc
    25921 tgcgcgtggt gccgaccatc tgtttcgatc tgcggttccc ctaccttttc tgggacgcgg
    25981 ccgaacaggc ggacgtcttc atcaacatcg ccagctggcc cgcccggcgc tcggaacatt
    26041 acaccaccct ggtgcgcgcc cgcgccatcg agaatcagtg ctacgcgctg ggcgtggacc
    26101 gcaccggcaa ggaccctctt ctggaataca gcggcaactc catgctgttc gaccccctcg
    26161 gcaaagtcct tttggattgc ggcacacaag acggcgtttt cgtgtccgat atcgacgtta
    26221 ccagggactt ggtgaccaaa acgcgcacgc ggtttccgtt cttcagggac aaaaaggcgc
    26281 cgggcgcgta cgagtgcctc aagaaaaact agcggattct tgaaaaggtc cggggcgccg
    26341 aggtcccagt gcgactgatc ggttacggag attccgtccg tcccggcgtt tatcgtcgcc
    26401 attccgcatt cgaaaaagcc gtcaacttca ccgacggcga acggctcgtc tctttggtgg
    26461 cggaagacgt gggccggggc cccatcaatc tggtctttaa agatttcaac gcaacgctct
    26521 gtcgcggtcc gctgaaagtc cgaagaacgt ctctctctgt gggc
//
LOCUS       DAOTFD010000015        26359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1206_length_26359_cov_5.080178, whole genome shotgun sequence.
ACCESSION   DAOTFD010000015 DAOTFD010000000
VERSION     DAOTFD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 26359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26359
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_26359_cov_5.080178"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="P5079_01900"
     CDS             <1..635
                     /locus_tag="P5079_01900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HRY28772.1"
                     /translation="FRSLSGRPLLYWPLRSFEKSPSVKQVVLVVSAARLPWARRFLRE
                     CRFWKVTAVVAGGRERMDSVLRGFAAVPSSTDVVLFHDAARALVSPGVIERVARSARR
                     TGAALAAWPVPDTVKLGRAPRRGKVLVARTVPRRNLWLAQTPQGFTRAVAEKIFRRSR
                     RNITDDVQWAERAGRSVEIVPGSPENLKVTLPEDFKLCEALLAARKRVSR"
     gene            640..1125
                     /gene="ispF"
                     /locus_tag="P5079_01905"
     CDS             640..1125
                     /gene="ispF"
                     /locus_tag="P5079_01905"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRY28773.1"
                     /translation="MTRTGWGYDSHRFIKGRRLVLGGVLVPFEKGLKGHSDADVLLHA
                     VIDAVLGACALGDIGTLFPDTDPRFKDVSSLFLLREAVRLVSKRFSIRHIDVTLLAEA
                     PRLGPYRQRIRAKVARAAGLPVEDVSVKAKTNEGMGFVGRREGLAAAAVATVEDRPRR
                     R"
     gene            1122..2552
                     /gene="cysS"
                     /locus_tag="P5079_01910"
     CDS             1122..2552
                     /gene="cysS"
                     /locus_tag="P5079_01910"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY28774.1"
                     /translation="MTPVPLLLHNTLSGREEPFQPVEEGRVKMYVCGVTPYDVCHLGH
                     ARCYVAFDFIRRGLRHLGYDVEYVQNFTDVDDKIIKRAAERNEEPSALTERYIADYFE
                     KMDALNVLRAGHYPRVTQHIPQIVKFVERLVANGFAYPVEGDVYYEVRRFERYGRLSK
                     RSLDELQSGARVGVDERKRDPLDFALWKAAKPGEPSWDSPWGPGRPGWHIECSVMSIA
                     SLKTETFDIHGGGQDLVFPHHENEIAQAEGATGKTFARYWMHNGFVTVNKEKMSKSLG
                     NFFSLADIFKKYEPRVVRLMLLSQHYRSPLEFSDKLLDMAAATLAHVEGTLSRALSFV
                     NTAGSAAGGELPLDYKKYFEEQKNKLTEALADDFNSPKALSVVFGAVQTLSTYQNLRQ
                     PVSDLVLKTYSDFVKETLDNVFGLRLNIEEVVEDRFVNDMLLRRERAREEKNWSEADR
                     IRKELADRGITIEDTPTGSRLLKKKT"
     gene            2549..3289
                     /gene="rlmB"
                     /locus_tag="P5079_01915"
     CDS             2549..3289
                     /gene="rlmB"
                     /locus_tag="P5079_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRY28775.1"
                     /translation="MNPAEDLVFGRKPVLELLTAGGARSVVKLWLLRGLAGPPVDELV
                     QRAKEKNIVFQWVDRQRLNQLTSGKNHQGVVARVSPFQYADLSDFLGASGAPSGLLLL
                     DGIEDPHNLGAILRSAAFFGAPAVIVPRWRSAGLSGTVAKTAAGALEKVPLIQVSNIA
                     QTIADLKKRNYWVYGADMKGDPCDRTTLNEPYALVIGAEGAGLHRLVRERCDALVSIP
                     GGGGMESLNASCAAGVFLYELFRRRRSA"
     gene            3286..5445
                     /locus_tag="P5079_01920"
     CDS             3286..5445
                     /locus_tag="P5079_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28776.1"
                     /translation="MNFVPWLRKNKGTAALLLALWGWHVLLLTTWLHRDQRPLQWDDA
                     VHASTAWDYKEAARSFSLFDLLRTPPRPGHPPYPPLVHYGIASFLAVSQGIGVPMEDA
                     ATWVNLVFIGILILGAWLLASTWWGNTAGLCAAVLVSLAPPVLMHSRDILVDVALASW
                     VVMTYALWMKSAAFTKRKITFLLGLVFAGGLLTKWTFPVYVLPVLAAAVRAAAKPKDD
                     SIRRSLLYGAGVAFILAAPWYAVNAFTVLPRLIGVASQGSLEGDPAVLSLSSWVWYLK
                     LLPQQLSWPGLLLCLGGVAWIFYRRLPGRGRLAVWFFVGYLVWSAVSNKNDRYFLPVL
                     LVLPLSASALPYRIPQASALAAVLFSAWFTWGPAGSAFALPPAAQSWPLEEIISAAAQ
                     DRDPAAPFSILTVVSNHQYLNGNNLRWTAQKTGVLDKIVPRTKLQSLGRLSEFVLVKT
                     GDLGPVYSIGRTLSAREEIFQPDGWFTRSFDVRRRWSLPDGSEAILYRRREPEASREG
                     GLSMETVASLAADAELRRFSMDVEPSARRGGEYACRLRAGQVSFGGITLRDVDVTLDG
                     AQTGKDDQGKTRLLRLRRVHVSSARFFESDAAAFFRTKIKNLEDASVVFLSDRRIALS
                     GRAAGIPLYAEVAVRYSSEREFPILSGRILKLKAAGLPLPVFLLGPRRTVALELKPHP
                     GLPFELTLAGLEVVPSRAGGEGALVITQNQKEGRENR"
     gene            5442..6395
                     /locus_tag="P5079_01925"
     CDS             5442..6395
                     /locus_tag="P5079_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY28777.1"
                     /translation="MTRLSIVVPVFNEEEVVSEFYRRLKQVLRSMDIAHEIIFVDDGS
                     RDNTVSLLLKQAAGDDTVKVIEFSRNFGHQNAISAGLDHAAGDACIILDGDLQDPPEL
                     IPEMVSKWREGHEVVYAVRNAREGEGFFKKGTAAVFYRLLRTLAGVDIPKDTGDFRLM
                     DRQVVDALGRLPERNRFLRGLVSWVGFRQVGIPFDRPARLAGETKFSLFKMLRFALDG
                     ITSFSRLPLRLVTLVGLFCFLGSLAVLGWAFYVRLFTVRSVQGWTSLIGVVLFLGGTQ
                     LLATGVVGEYIARIFDEAKQRPLYLVRRTHGFPPGRGTPQG"
     gene            6405..7130
                     /locus_tag="P5079_01930"
     CDS             6405..7130
                     /locus_tag="P5079_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28778.1"
                     /translation="MPRLWFQVRRYVILFEGRTGSSYLTELFWSHPRIRARSEGLAAI
                     YHDGGTAAEQLCWARAALTPPVLGRFSAVGFKTKVHEMFDPRRMAELFRAKNAHVFHM
                     QRRNRIKLLLSEINALRLSQKTGQWNIYREEDRLPPVRVDPEEFRFLLKGREEREQKL
                     TDYVAALGLPTLPVFYEDLVREKEKTLDRIFSFLGIEPRALRAKMIKHNSEDLREVIE
                     NFDDLRSRFVGTPYEPMFDEGAK"
     gene            7127..8506
                     /locus_tag="P5079_01935"
     CDS             7127..8506
                     /locus_tag="P5079_01935"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRY28779.1"
                     /translation="MTISIFDLFAIGVGPSSSHTVGPMRAARRFAETLRNGGRFAEVS
                     RLRVELFGSLGVTGKGHGSDHAVLLGLEGEHPETAVPEDISVRVAAIRKAKRIQLLKT
                     RSLPFEESRDLIFNQNDRLPLHSNGLKFTAFDGARDEIASRVYYSVGGGFVVDEETAG
                     QNRFSGDETAVPYPYRSAADLLRQGRVTKKAISEIVFENEKVRRTPEQIQEGLMGIWQ
                     AMRACVERGLRGAGELPGGLRVPRRAPGLYQTLVSRPEDSFKDPLTVLDWVDVYALAV
                     SEENAAGGRVVTAPTNGAAGIVPAVLHFYKKFTPNATDDGVRRFLLTAGAIGILYKES
                     ASLSGAEVGCQGEIGVACSMAAGALAAVLGGTNEQVENAAEIGMEHNLGLTCDPVGGL
                     VQIPCIERNAVGAVKAINAARMALRGTGQHKVSLDSVIATMRQTGLDMQTKYKETSRG
                     GLAINVIEC"
     gene            8729..9643
                     /locus_tag="P5079_01940"
     CDS             8729..9643
                     /locus_tag="P5079_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28780.1"
                     /translation="MRKQGNIVLVGMTMLIGITVGSRAAINWEEMGRHGMMPPKTYNF
                     DSAKKIINATLEMTARAREAWWMANGMLGASRIPANTPKGSCACRGYNSLFSSGFASS
                     QNTKMERVATGPCLDMLVLTDHPGCNSWVCLRERYIGRIGYYLNTRFQVDGRGFPGRI
                     LSSVREHKSHEELTKFLGDAYALYRRLEGRGYEDRTLGDAIGIVSAFLDYDGLMPSAS
                     GIREIMYLPATSQTYADTARSVIEDMRRGQTFYSALAVEMGKRNLHRNDIIAFPDDEA
                     QHKVLLSLIREDLNGLEKTINAAAVSGD"
     gene            9673..10047
                     /locus_tag="P5079_01945"
     CDS             9673..10047
                     /locus_tag="P5079_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY28781.1"
                     /translation="MRKKTVAVVGASADPSKYGNKAVRAHVKAGYDVYPVNPTAELIE
                     GLKVYRNVLDIPVELDRVSVYLPPEKGVRVLEDIACKGTKELFINPGAESPRLIARAR
                     TLGLVVILGCSILSLGEDPQTL"
     gene            complement(10089..10241)
                     /locus_tag="P5079_01950"
     CDS             complement(10089..10241)
                     /locus_tag="P5079_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28782.1"
                     /translation="MSATLRKPEATPGGIGSGVYRPRGIGSGVYRPRGIAPSDKLGRY
                     LAASRF"
     gene            10326..10398
                     /locus_tag="P5079_01955"
     tRNA            10326..10398
                     /locus_tag="P5079_01955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10359..10361,aa:Thr,seq:tgt)
     gene            10446..12026
                     /locus_tag="P5079_01960"
     CDS             10446..12026
                     /locus_tag="P5079_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY28783.1"
                     /translation="MKRLALALMFCLAGCITFPTDPQSAVPEPEPAFLTADRDAAPVL
                     VNLLDERKLESPVGAEFSSTILKDIVRLTTPTGYRLKNRYLKLGVPLAQALYFAKDAQ
                     LQNRLLEAARWTSSPRARSEALVVLAKQRNPEHLKYFREALLDSNVAIQFAAIEALQV
                     WAGEGASELLLSVANQNWSPVIRVYAAQAALRLGSSAGRDKLLEFLRERNWLMRAMAA
                     RYLGDMGRPEDGDLLLSRIGPEQDNKFVLAEVCIAALKLTGRRLPASMAPPPPPSPRA
                     PLKPTRQPFELEPLVVTAPRLKLMQHVDVRIDNDLVRLLEKMASEPIEVEKVVDPDVA
                     QLSRIETPVGFSLAVRYNDLIFLLIEGLAGSRDLGLVRRLEEIARNNSNANVRAAALV
                     ALGYDRDRTDLAIFQAALRDATNTSVRFAAVEALGQIGGSQVIGTLADVANSDRSRAV
                     QIFAAQALRRSGDPYGREILLRALDDADPYARAMATYYLGEVGEPDDYNRVLFRMNNE
                     SNNFAVAEACLALYRLSP"
     gene            12055..12705
                     /locus_tag="P5079_01965"
     CDS             12055..12705
                     /locus_tag="P5079_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY28784.1"
                     /translation="MTEFGALKVLAYSGGEWVIYLLLACSILTIAVIIERALVLRRER
                     RQLCGLEKELATLLEKGNLESAASVSAGAQGAAARLLSSGLGHLKAGPAAVEEYIASA
                     KITEKQAMERRLLILGSLGNNAPFIGLFGTVLGVIKAFHDLAETAGAGPEVVMQGLSE
                     ALIATAVGLLVAIPAVMAYNYFQKRVNDFFADIESLSRLLLARLRDESPSTKSSGR"
     gene            12709..13116
                     /locus_tag="P5079_01970"
     CDS             12709..13116
                     /locus_tag="P5079_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRY28785.1"
                     /translation="MAGPAGNSENSLITGINITPLVDVVLVLLIIFMATAPLLMRRAL
                     SVNVPRAAHNEPKATATLQIQMTAVGEVSVEKKKLDLEQLDRMLRERLLTDPALHVSL
                     AADKTIPYGEVVGVMDVVRGAGVKKLALEVKSK"
     gene            13121..13828
                     /locus_tag="P5079_01975"
     CDS             13121..13828
                     /locus_tag="P5079_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY28786.1"
                     /translation="MPAIPFGAFLRQHRLPPFETALLVSFFLHVAAWQALTWKERFVR
                     QEEVDRLEIDLTRPFRLTDNPFLARRAKVSGTGAPIVEKPKPVTAPPAGEVKPPSEWV
                     LPGPDTKELEKPVTAEDPSGLGGFGVGEGGGEVDWVYLTSLPQLLNRDALLRNLRRYY
                     PESERRAGREGQVVLDLHIDRQGSVTAVDVVTSAGAAFDAAAEKVIRLARFSPAKVET
                     RAVPVKIRQTVAFQLEE"
     gene            13882..14181
                     /locus_tag="P5079_01980"
     CDS             13882..14181
                     /locus_tag="P5079_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28787.1"
                     /translation="MAERRTIEISCPCCQAVLTVDAETGDVLLHKEAKKKVEMDIAKA
                     AQALKDDVSRRDDIFRKSIEAQKRKGGRLDKQFEEGLRRAKEEPETPRLREIDLD"
     gene            14263..15090
                     /locus_tag="P5079_01985"
     CDS             14263..15090
                     /locus_tag="P5079_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY28788.1"
                     /translation="MAHIIAIANQKGGVGKTTTAINLAASLAHYGHETLLIDMDPQAN
                     LSSGLGLTHDKVTKHIYHVLLQDTPLEEILRETGVEWLDVAPSHLDLYGVEVELVNAE
                     HRERRLQMALEKFQKAYKFIFIDCPPALNLLTLNALVSAATVLIPMQCEYYAMEGLSL
                     LIQTIERVRGGLNPRLELEGVLMTMYDSRTNLVQQVHQEIKKFFGNKAYKTVIPRNIR
                     LAEAPSHGLPALGYDKHSTGAEAYLALAREFLRRHGADAEHVETASPEPVSAAPAGA"
     gene            15112..16008
                     /locus_tag="P5079_01990"
     CDS             15112..16008
                     /locus_tag="P5079_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY28789.1"
                     /translation="MEHKPLGRGLEALLKQGTSTLSAADRESVAKIAVDKIRPNRYQP
                     RVHFSSESLQDLSASIKAHGLAQPLLVSPSAVPGEFELVAGERRLRASKMAGLSEVPC
                     VVRAVSERQRHELSLVENIQREDLNAVEEAEALKKLMSEFSLTQEEVAHALGRSRSAV
                     ANKLRLLELPEDIRQAIMVGQLSEGHARTLLGIEERSRQTELSRRILREHLTVRDVEK
                     IVADWQSAHHEGRVKTTKRKDPDVRQLEEDLQRTLGRKVEIQTRGKKKGWLRLAFYSL
                     DDLQLLIRHLKKSKDGHSGRGA"
     gene            16146..17135
                     /locus_tag="P5079_01995"
     CDS             16146..17135
                     /locus_tag="P5079_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28790.1"
                     /translation="MPSAPAETPLFSVPDDKSPVYLTEISNPNDFVLFANGGWDGNWY
                     VGYNTCWVSKLPPAPPGEYKSAFLGARLGRMKTESVPGRPPWEKRPIKGFINMAVADE
                     PLWPQSRRFRLTETADIPLEGDAENAVEGVGESRWFWVEVPLKFISFEEDNYVALFSP
                     SEALSSSARAPVLAAAWGDTRQNTWLNSTVKGEPPFTSTDALKTPVTYFEPAIAIKLV
                     PVNDRRPDIRLLETPEEGATVQEKIFVSASVSGRDMAGVWVEFSTDTKNWRRAGSFLA
                     IAPYVFTVKREQLLEGTVRLRVAAEDVMGNRAQSPIFSVRVPPPPSEPPKKRR"
     gene            17156..18061
                     /locus_tag="P5079_02000"
     CDS             17156..18061
                     /locus_tag="P5079_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRY28791.1"
                     /translation="MKPTISASLRAPVFAVGLFLAVAAGAGESLPTQSYRFLDWTVPH
                     RVEEAYIQYVYDGDTVQDRYQRRIRLLGVDAPEVAHPEHDKFTNEPGGIEALHFTENK
                     VRGKKAYLAIDDENAVDKYGRTLAVVFVKDDLGEWSCLNWDLVREGMAEVMILPGNRF
                     CVEAEWHKLSAVASRRRAEDFRELAQSFKDEGRSESAVETYQRGIRLFPDARALYEDL
                     AGLYLGMNLPGFSVDIYLAYLERHPGDVLIRYRLARAYELMAAVQSLPLVDYRKKSTE
                     EWGRLLGTEYDAEARQTLLRLEGKP"
     gene            18087..18512
                     /locus_tag="P5079_02005"
     CDS             18087..18512
                     /locus_tag="P5079_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28792.1"
                     /translation="MPRPNFLKRKYLVNKPLQLKYAFWIGAALFVLMLLAQAHTYFTI
                     ESILPNLFSSALGRQARSLQTWLLLNSLIYLAVVVILSVFLSHRIAGPVYHFEKEIRE
                     VLESNDPSRRITLRKGDELHSLADQINKLLERLAQSQRR"
     gene            18526..18729
                     /locus_tag="P5079_02010"
     CDS             18526..18729
                     /locus_tag="P5079_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28793.1"
                     /translation="MKRLSLIIFFLVVLGCSKSPEGVSNTATRYVDGLQADVEKAKDA
                     AAAANKVIADTQAMMKKAGEAVE"
     gene            18761..19864
                     /locus_tag="P5079_02015"
     CDS             18761..19864
                     /locus_tag="P5079_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY28794.1"
                     /translation="MRVLHVTESRSWSGGTVQLWHLCQALARHGHAAALFCPPESELL
                     RHAAGSDVAVTVCPMRQDYDVFAARALAEAIRRFRPDIVHAHHPRAHALALLAGFFVD
                     IPRLVVSRRVSFRLKKWNFFSQWKYRTSRIRLFVAVSDDIRRVLVAGGVPEERVEVIQ
                     SGVDVARFSPRPPDEKLRTELAFPRGTPIVGNLTHFSWWKGQTFFLEAARLVLSSGVP
                     AHFLLVGKDTDGPAARAKVTELGIEKNVTLAGFRTDMPEVLSLLGVSVVSSLAGEGFS
                     GVLRESMSMGIPVVATDVGGNKELVLNEKTGLLVPPGDARSLADAVRRLLTDKTLADS
                     CARAGQESVRSRFSIGKTLERTEKLYERLLAER"
     gene            19866..20675
                     /locus_tag="P5079_02020"
     CDS             19866..20675
                     /locus_tag="P5079_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28795.1"
                     /translation="MSFFKKTLVAAWVMVPGIAVAGEISLMYLPQSQIEYEGTGYRRF
                     DEGWELKVKPQSAAAGFLWRHPLSDRWDLQAQFWRNQSFFAREDGNAKSDLMRQTGQT
                     ELTVNNLILDVRRPLAGSSVRAVAGLQGVYENFHRKEILFNAVPEAGDSRENLSAAGA
                     VLGFAGASPDSTDRNRFYCGWEALFGHFFYTRTSHAIDGGSIHRDGYSYALRLETGYQ
                     MRKWRLGAGFVRQLIQVQVPGGRTFPGGAAASLPINKTDFFGLFLALSHVY"
     gene            20683..21519
                     /locus_tag="P5079_02025"
     CDS             20683..21519
                     /locus_tag="P5079_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28796.1"
                     /translation="MKPAAFLWVILALLPVRASADDGLRWNLTVLPHASLRYRGEGKV
                     LPESYVNRFAGETSPIYRFELTQVPRSDGYWGVSFWHTGVFGGGSYAEERVPDNTTGG
                     AYQINQLNVGFTNLFVTYHRPVAALPVEARVHASVVREIFKRKQFIVQNVDYRWTNFD
                     DVNEISAEGLGFGLAGRHGGRFYLRWQADAHYYIQLFDAKTDSSAGQIYQSEAALGAR
                     VLPRLSVEFGALWQYWFTHSQGNRRLVIPGTDGAVISWNRQETRASGAFLRLDYFFAG
                     RD"
     gene            21574..22929
                     /locus_tag="P5079_02030"
     CDS             21574..22929
                     /locus_tag="P5079_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRY28797.1"
                     /translation="MKKKPIKYARRGFFAALTVFAAVLVAEEPGKPASASGPVYVLNI
                     DGTIDPVIAKYVADAFAEAKEENAAAVVIRLDTPGGLLDATRDIIQQILNAPMPAIVY
                     VSPRGSRAASAGVFITVASDVAAMAPETHLGAAHPVSLGGNPFMPAKPADPEKNGKED
                     EGRSVMEEKIVSDTAAYARTLASAKGRNADWVEKAVRESLSLTSTEALEKNVVDMLAE
                     DETDLFKKLSPRDIKKNEKVFRIDFEKARMVRFEMGRLRQVLHVLANPNVAYLLLMLG
                     FYALIYEFATPGIGLGAAAGLISLVMAFFALQVLPLSYAGLALLLAGMTLMAMELFVV
                     SHGLLMVGGTLCFIAGSLLLFESPEPYFRVSLEIIGGTAVASLAFLVFVVKKILAVRV
                     MASITGKEALVGQIGEARPDGMVFVHGEYWEAESAAPLSPGQKVRVVEVMGNRLRVEK
                     V"
     gene            23038..23781
                     /locus_tag="P5079_02035"
     CDS             23038..23781
                     /locus_tag="P5079_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRY28798.1"
                     /translation="MPIIIIAAIIIINAVKIVQEYERGVIFRLGRVVGAKGPGLFFII
                     PIVDRMVRVGLRTVTLDVPVQEVITKDNVPCKVNAVCYFRVLDPVKSVIEVQDYIIAT
                     SQIAQTTLRSVLGQAEFDELLSQREKINTKLQHIIDQQTDPWGIKVSIVEVKDVQVPE
                     QMQRAIAHQAEAERDRRAKIIAAEAEYQAAQKLADAAQIISSQPVSLQLRFLQTALEI
                     SNQKNSTLIFPLPIELFKSFGAYLEKARN"
     gene            complement(23853..25064)
                     /gene="thiI"
                     /locus_tag="P5079_02040"
     CDS             complement(23853..25064)
                     /gene="thiI"
                     /locus_tag="P5079_02040"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRY28799.1"
                     /translation="MADMNDILLVHYSEIGTKGKNRSFFERRLRNDIEKRLGPDAEGV
                     RVESGRVVVRLSPGFEKGRLESHLNQVFGVAWYALAQSVPREWEPILQAGLDCAAAHP
                     AARAFKVYSHRADKTFPLSSQEICNRLGRAVVEKYGLKVDLERHDLAIYVEILADEVF
                     VYSERKPGLRGMPQGSSGRVLCLFSGGIDSPVAAWLLARRGAMVHLLHFHPFRRAEEL
                     RDTKIFSLHKQLLLYSPGSRLYLIPHYHFQLKAALDVPSMYEMVLFRRFMFKTAEKLA
                     ERRRCKALVTGDSLGQVASQTLENIAAAQADLKIPVFLPLISQDKDDIVRLAQKIGTY
                     DLSIQPYKDCCSLMSRHPKTMMPVHKALELEKKINLDAVVADSLASLELWDGETISAP
                     ALSFKNQPLSN"
     gene            complement(25134..26060)
                     /locus_tag="P5079_02045"
     CDS             complement(25134..26060)
                     /locus_tag="P5079_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRY28800.1"
                     /translation="MLPSWLKRLLGILLLVTAATIAVKIPRPAPKPPLPELPASPEKL
                     EIVRPDARLLFQKSGDEWTLQQPLDYPADAETLKLLFSSLSSLSTDERLTDRAETHSL
                     YEVNDSSAILVRVWGPQGKEPAEWLFGKTAPDFLHIYLRRPGRPEVYLAKGVERDALT
                     QPLARWRERRLFKTDPADGIHRLVFRREKTALELLRASDTWTLAGKTAETSAVENFLA
                     SLRVLSVEEFIDPPASSDTKKFGLLTTKNSLEVLFSSGKTVRLDAGEKDAVQNRYPLR
                     RNEEPSLFWISANDWDGLFSGLQRLRPADNRK"
     gene            complement(26062..>26359)
                     /locus_tag="P5079_02050"
     CDS             complement(26062..>26359)
                     /locus_tag="P5079_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28801.1"
                     /translation="FGTAFYAAPHMPVPETNLTTLASLSEWLAQDPNFLSIPSKNSPF
                     RPIRPLSDPAKQGFKLAGYFLLPVVVLAGGLWRWQNRRKQRERVRSLFQRNPAT"
BASE COUNT         4825 a         7800 c         8543 g         5191 t
ORIGIN      
        1 ggttcagatc cctctccggt cgtcccctcc tgtattggcc gctccggtct tttgaaaaat
       61 cgccgtcggt aaagcaggtg gtgctggtcg tctccgcggc ccggctccct tgggcgcgac
      121 ggttcctgcg ggaatgccgt ttttggaaag tgaccgccgt ggtggcgggc ggccgggagc
      181 gcatggattc cgtgttgcgg gggtttgcgg cggtcccgtc ctcgacggac gtggtgctgt
      241 tccatgacgc ggcccgcgcc ctggtgtcgc ccggcgtcat cgagcgggtg gcccggtcgg
      301 cccgccgcac cggggcggcc ctcgccgcct ggcccgtgcc ggacaccgtc aagctcggcc
      361 gcgcgccgcg ccggggaaag gtcttggtgg cccgcaccgt gccccgccga aacctctggc
      421 tggcacagac cccgcagggt ttcacacggg ccgtggcgga gaagattttc cggcgttccc
      481 ggaggaacat caccgacgac gttcaatggg ccgaacgcgc cggccggtcg gtggagatcg
      541 tgccggggtc cccggagaac ctgaaggtga ccctgccgga ggatttcaaa ttgtgcgaag
      601 cgcttttggc cgcccggaaa cgggtttcga ggtgagttca tgacacggac gggttgggga
      661 tacgacagcc atcggttcat caagggccgc cgccttgttt tgggcggggt actcgttccg
      721 ttcgagaagg ggctgaaagg ccattcggac gcggacgtgc tgttgcacgc cgttatcgac
      781 gccgtgttgg gcgcctgcgc cctcggcgac atcggcacgc ttttccccga taccgatccc
      841 cgatttaagg acgtttccag tctttttctc ctgcgcgaag cggtccgtct cgtttccaaa
      901 cgtttttcca tccgccatat cgacgtcact cttttggcgg aggccccgcg tctcggcccc
      961 taccgccagc ggatccgcgc caaggtggcc cgcgccgccg gcctccccgt ggaggacgtc
     1021 agcgttaagg ccaagacgaa cgagggcatg ggttttgtgg gccgccgcga aggcctggcc
     1081 gccgccgccg tggccaccgt ggaagaccgt ccccgccgtc gatgacgccc gtcccgctcc
     1141 tcctccacaa taccctctcc ggccgggagg agcctttcca gccggtggag gaaggccggg
     1201 tgaagatgta cgtgtgcggc gtcacgccgt acgatgtctg ccatctgggc cacgcccgct
     1261 gctacgtcgc ctttgatttc atccgccgcg gcctgcgcca tttgggctac gacgtcgaat
     1321 acgtccagaa tttcaccgac gtggacgaca agatcatcaa gcgcgcggcg gaacggaatg
     1381 aggaaccgtc ggcgctcacc gagcgctaca tcgccgacta tttcgaaaaa atggacgcgc
     1441 tgaacgtctt gcgcgccggt cactaccccc gcgtcacgca gcacatcccg cagatcgtca
     1501 agttcgtcga gcggctggtg gcgaacggtt tcgcctatcc ggtggagggc gacgtctatt
     1561 acgaggtccg gcggttcgag cggtatggtc gactctccaa gcgttccctc gacgagcttc
     1621 agtccggcgc ccgcgtgggg gtggatgagc gtaaaaggga tccgctcgat ttcgccttgt
     1681 ggaaggcggc caaacccggg gaaccctcct gggactcgcc gtggggcccg ggccgccccg
     1741 gttggcacat cgaatgttcg gtgatgagca tcgccagcct gaagaccgaa accttcgaca
     1801 tccacggcgg cggccaggac ctggtttttc cgcatcacga gaacgagatc gcccaggcgg
     1861 agggcgccac cggcaagact ttcgcccgct actggatgca caacgggttc gtgacggtca
     1921 acaaggagaa gatgtcgaag tccctgggga attttttctc gttggccgac atcttcaaaa
     1981 agtacgagcc gcgcgtggta cgtctgatgc tgctgtccca gcattaccgg tcccccctgg
     2041 agttttccga caaattgttg gatatggctg cggcgacttt ggcgcatgtc gaggggacat
     2101 tgagccgtgc tctgtcgttt gtgaatacgg caggatcggc tgcgggaggg gaactgccgc
     2161 tggattacaa gaaatacttc gaggaacaaa agaataagct gacggaggcg cttgccgacg
     2221 atttcaattc ccccaaggcg ttatcggttg tttttggggc cgttcaaaca ttgtccacgt
     2281 atcagaattt gcgacagccg gtgtcggatt tggttcttaa aacttactcc gatttcgtga
     2341 aagagacact cgataatgtt tttgggttaa gactgaacat cgaagaagtc gttgaggatc
     2401 ggtttgttaa cgacatgctt cttcgacggg aaagagcgcg ggaagagaaa aactggtctg
     2461 aggccgaccg gatacggaaa gaactggccg accgcggcat caccatcgag gacacgccga
     2521 cgggatcgcg cttgctgaaa aaaaagacat gaatcctgcc gaggacctgg ttttcggccg
     2581 gaaacccgtt ctggaactgc tgaccgctgg cggcgcgcgg tccgtggtga agctgtggct
     2641 gttgcggggg ctggccggcc cgcccgtcga cgagctcgtg caacgcgcca aagaaaagaa
     2701 catcgtcttt caatgggtcg accggcaacg cctgaaccag ttgacctccg ggaaaaacca
     2761 tcaaggggtc gtggcccgcg tcagtccttt tcaatacgcc gacctttcgg attttttggg
     2821 ggcttccgga gccccttcgg gactcctcct cctggacggc atcgaggacc cgcacaacct
     2881 cggcgccatc ctccgcagcg ccgctttttt cggcgccccg gcggtcatcg tgccccgctg
     2941 gcgctccgcg ggactgagcg gcacggtggc caagaccgcc gccggcgcgc tggagaaggt
     3001 gccgctcatc caggtgtcca acatcgccca gaccatcgcc gatttgaaga aaagaaacta
     3061 ctgggtgtac ggggcggaca tgaaggggga cccctgcgat cggacgaccc tgaacgagcc
     3121 ttatgcgctg gtgatcggcg ccgagggcgc gggtctccat cggctggtcc gcgagagatg
     3181 cgacgccttg gtgagcattc cgggcggcgg cgggatggaa tccctcaacg cctcctgcgc
     3241 cgccggcgtg ttcctctacg aacttttccg tcgtcgccgt tccgcatgaa cttcgtccca
     3301 tggctgagga aaaataaagg gaccgccgcg ctgctgctgg cgttgtgggg ctggcacgtt
     3361 cttttgctga ccacctggct gcatcgcgac cagcgccctc ttcagtggga cgacgccgtc
     3421 catgcgtcca ccgcgtggga ctataaagaa gcggcccgct cgttctcctt gttcgacctg
     3481 ctccgcacgc ctccccgtcc cgggcacccc ccttatccac ccttggtcca ttacggcatc
     3541 gcgtcgtttc tcgcggtttc ccaggggatc ggggtgccga tggaagacgc ggccacctgg
     3601 gtcaaccttg ttttcatcgg gattttgatt ttaggggcgt ggcttctggc gtcgacctgg
     3661 tgggggaata cggcgggact gtgcgcggcc gtgctggtga gtctggcgcc tccggtgttg
     3721 atgcattcgc gggatatttt ggtggatgtg gcgctggcgt catgggtggt catgacctac
     3781 gccttgtgga tgaaaagcgc cgcttttacc aaacgaaaaa ttacttttct cctgggcctc
     3841 gttttcgccg gcggtcttct gacgaaatgg acgtttcccg tctacgttct gcctgttttg
     3901 gccgccgccg tccgcgcggc ggccaaaccg aaagacgact cgattcggcg ttcgttgctt
     3961 tacggcgccg gggtcgcgtt tatcctggcc gccccctggt atgccgtgaa cgcgttcacg
     4021 gtccttcctc gcctcatcgg cgtcgcgtcg caggggtccc tggaggggga tccggcggtg
     4081 ttgagccttt cgtcatgggt gtggtatttg aaactgctgc cgcagcagct gtcttggccg
     4141 gggctcctgt tgtgcctcgg cggggtggcg tggatctttt atcgccggtt gcccggccgg
     4201 ggcaggctgg ccgtttggtt ttttgtggga tacctggtgt ggagcgcggt ctcgaacaag
     4261 aacgatcgat atttcctccc ggtcctcctt gtcctgccgt tgagcgcgtc ggcgctgccg
     4321 tacaggattc cccaggcgtc ggcgctggcc gccgtgctgt tttccgcctg gttcacgtgg
     4381 gggcctgcgg gttcggcttt tgcccttccg ccggcggccc agtcctggcc gctggaggaa
     4441 attatttccg ccgcggcgca ggatcgcgat cccgccgcgc cgttctccat actcacggtg
     4501 gtgtcgaacc accaatacct caacggcaac aatctgcgct ggacggccca gaaaacgggc
     4561 gtgttggaca agatcgttcc gcggacgaaa ttgcaatcgt tgggccgcct gtctgaattc
     4621 gttttggtga agaccgggga tttggggccg gtttacagca tcggccgcac gctgtcggcc
     4681 cgggaagaga tatttcagcc ggacggctgg ttcacccgct ccttcgacgt gcggcggaga
     4741 tggtccttgc ccgacggcag tgaggcgatt ctgtatcgcc gtcgggagcc cgaggcctcc
     4801 cgggagggtg gtttgtccat ggagaccgtg gcctcgctgg cggccgacgc cgagttgcgg
     4861 cggttttcga tggatgtgga gccgagcgcg cggcgggggg gggaatacgc ctgtcgcctg
     4921 cgcgccgggc aggtctcgtt cggcgggatc actttgcgcg acgtggatgt gaccctggac
     4981 ggcgcgcaga ccgggaaaga cgatcagggg aagacacgac ttttgcggct gcggcgcgtg
     5041 catgtctcct cggcgcggtt cttcgaatcg gacgccgccg ccttttttcg gacgaaaatc
     5101 aaaaacctcg aggacgcgtc ggtcgttttt ctttccgacc gccgcatcgc tctctcgggc
     5161 cgggcggccg ggatccccct gtatgccgaa gtggccgtcc gctattcttc ggaaagagag
     5221 ttccccatcc tctcgggcag aatcctaaaa ttgaaggcag cgggactgcc gctcccggtt
     5281 tttcttctgg gaccgcggcg caccgtggcg cttgagttga aaccgcatcc gggcctgccg
     5341 ttcgaactga ccctggccgg actcgaggtg gtcccttctc gggcgggcgg agaaggcgcg
     5401 ttggtgataa cgcaaaacca aaaggaagga cgggagaacc gatgacgcga ttatccattg
     5461 tggtcccggt ttttaacgag gaagaggtcg tctccgaatt ttatcgacgg ctgaaacagg
     5521 tgttgaggtc catggacatc gcccacgaaa ttatcttcgt ggacgacggc agccgcgaca
     5581 acaccgtttc cctgttgctg aaacaggccg cgggggacga cacggtgaag gtcatcgaat
     5641 tctcccgcaa tttcggccac caaaacgcca tttccgccgg gctggaccat gccgccggcg
     5701 atgcctgcat catcctcgac ggcgacttgc aggatccgcc cgaactcatc cccgagatgg
     5761 tcagcaaatg gcgcgagggc cacgaggtgg tctacgcggt gcggaacgcg cgggaagggg
     5821 aaggcttttt caagaaaggg accgccgccg ttttttaccg gctcctccgg acgttggcgg
     5881 gcgtggacat ccccaaggat accggggatt tccgtctgat ggaccgtcag gtggtggacg
     5941 cgctggggcg tcttccggaa cggaaccggt ttctgcgcgg gttggtgagt tgggtggggt
     6001 tccggcaggt ggggatcccg ttcgaccgtc cggcgcgact ggcgggggag acgaagtttt
     6061 cgcttttcaa gatgttgagg ttcgcgctgg acggcatcac ctctttctcg cggttgcctc
     6121 tgcggctggt gacgttggtc gggctcttct gtttcctcgg aagcctggct gttttggggt
     6181 gggcgttcta tgtgcggctc ttcacggtcc ggagcgtcca gggatggacg tcgctcatcg
     6241 gggtggtgct ctttctcgga ggaacgcagc tcctggccac cggcgtcgtc ggcgaataca
     6301 tcgcccggat cttcgatgag gccaagcagc ggccgctcta tctggtgagg cgcacgcacg
     6361 gtttcccgcc ggggcggggg accccccaag gatagattct tcccgtgccg cgtctctggt
     6421 tccaggtccg tcgttacgtc atcctcttcg agggtcgcac ggggagctcg tatttaaccg
     6481 agctcttctg gtcgcatccc cggataaggg cgcgttcgga ggggttggcg gcgatttatc
     6541 atgacggcgg cacggcggcg gaacaactct gctgggcgcg cgcggccctg accccgcccg
     6601 tcctgggaag attttcggcg gtgggtttta agaccaaggt ccacgagatg ttcgaccccc
     6661 gccggatggc cgaacttttc agggcgaaga acgcgcatgt cttccatatg cagagacgca
     6721 accggataaa actgctcctg tccgagatca atgccctgcg gttgtcccaa aagacgggac
     6781 agtggaacat ttatcgcgag gaggaccgcc tcccccccgt gcgcgtcgat ccggaggagt
     6841 tccgtttttt gttgaagggg cgggaggaac gcgaacagaa attgaccgac tacgtggcgg
     6901 cgctgggcct gccgactctc ccggtgttct acgaggacct ggtgagggag aaggaaaaaa
     6961 cgctggaccg gatattttct tttttaggga ttgagccgcg cgccttgcgg gcgaagatga
     7021 taaagcataa cagcgaggat ttgagggaag tgatcgagaa tttcgacgat cttcggagcc
     7081 gtttcgtcgg aacgccgtat gaacccatgt tcgacgaggg agcgaaatga ccatcagcat
     7141 tttcgacctg ttcgccatcg gcgtcggtcc gtccagctcg cacaccgtcg ggcccatgcg
     7201 ggcggcgcgg cgtttcgccg agacgttgcg caacggcggc cgtttcgccg aggtctcccg
     7261 cctgcgcgtt gaactgttcg gttccctcgg cgtcaccggg aaggggcacg gcagcgatca
     7321 tgccgttttg ctggggctgg agggggaaca tccggaaacg gccgtccctg aagacatctc
     7381 ggtgcgcgtg gcggccatcc ggaaggcgaa acggatccag ttgttaaaga cccgctccct
     7441 cccgttcgaa gaatcgcgtg atcttatttt caatcagaac gaccggctcc cgcttcactc
     7501 caacggcttg aagtttacgg ccttcgacgg cgccagggac gagatcgcgt cccgggtgta
     7561 ttattccgtc ggcggcgggt tcgtcgtgga cgaggagacg gccggacaga accggttctc
     7621 cggcgacgag accgcggtcc cgtatcccta ccgcagcgcg gcggatctgt tgagacaggg
     7681 ccgcgtgacg aagaaggcga tcagcgagat cgttttcgag aatgaaaagg tccgccgaac
     7741 acccgaacag atacaggaag ggttgatggg gatatggcag gccatgcggg cgtgcgtgga
     7801 gcggggtttg aggggcgccg gagaactccc cggggggttg cgggttcccc gacgggcgcc
     7861 gggcctgtat cagacgctcg tcagccggcc ggaagactcg ttcaaggacc cgttgaccgt
     7921 gttggattgg gtggacgttt atgcgttggc ggtgtcggag gaaaacgccg ccggggggcg
     7981 cgtggtgacg gcccccacca acggggcggc cggcattgtg ccggcggtcc tgcatttcta
     8041 caagaagttc acccccaacg ccacggacga cggggtccgg cgattccttc tgacggccgg
     8101 ggccatcggc atcctttata aggaaagcgc ctccctctcc ggtgcggagg tgggttgcca
     8161 gggagaaatc ggcgtggcgt gttccatggc ggcgggggcc ctggcggctg tcctgggcgg
     8221 gacgaacgaa caggtggaaa acgccgccga gatcggcatg gaacacaacc tcggtctcac
     8281 ctgcgatccc gtgggcgggt tggtgcagat cccctgtatt gaacgcaacg ccgtgggggc
     8341 ggtgaaggcc atcaacgcgg cgcgcatggc gttgcgcggc acaggccaac acaaggtctc
     8401 cttggacagc gtcatcgcca ccatgcgcca gaccgggctg gacatgcaga ccaaatacaa
     8461 ggaaacgtcc cggggcgggc tggccatcaa cgtcatcgag tgctgacagg gagaaacgcc
     8521 gcccggagcg gttgggggcc gggcagggca aaatcacact tgttttttcg gcggcactga
     8581 aatacaatcc atagtgtagg gcctaccttt agtaactggc attaaatcca tcaaaagacg
     8641 gacgttgagg gaaaaatgag accgctgagg cggttttcat gggcggcccc attttgtccc
     8701 gacaacaatc cccgtcaagg agtcagccat gagaaaacag gggaatatcg ttcttgtggg
     8761 gatgacgatg ctgatcggga tcaccgtggg gagccgtgcg gccatcaact gggaggagat
     8821 ggggcggcac ggcatgatgc cgccgaagac gtataatttc gactccgcca aaaagatcat
     8881 caacgccacg ctggaaatga cggcgcgcgc ccgggaggcg tggtggatgg ccaacgggat
     8941 gctcggcgcc tcgcgtatcc cggccaatac gcccaaggga tcctgcgcct gcagggggta
     9001 taattccctt ttttcgtcgg gcttcgcctc ctcccaaaac acgaagatgg aacgggtggc
     9061 gacgggtcct tgtctcgata tgctggtgct gacggaccat ccgggatgca actcctgggt
     9121 gtgtctgcgg gagcggtaca tcggccggat cgggtattac ttgaacaccc gctttcaggt
     9181 ggacggccgc gggtttcccg gccggatact ttcgtcggtg cgtgagcaca agagccatga
     9241 ggaactgacc aagttcctgg gggacgccta cgcgctctat cggcgtctgg aggggcgcgg
     9301 gtacgaggac cgcacgctgg gggacgccat cgggatcgtc agcgcgtttt tggattacga
     9361 cggactcatg ccttccgcca gcggcattcg ggagatcatg tacttaccgg ccacgtcaca
     9421 gacttacgcc gacaccgccc ggtcggtgat cgaggacatg cggcggggcc agacttttta
     9481 cagcgccctg gccgtggaga tgggcaagcg gaatctccac aggaacgaca tcatcgcctt
     9541 cccggacgat gaggcgcagc acaaggtcct cttgtcgctg atccgggagg acctgaatgg
     9601 gctggagaag accatcaacg ccgccgccgt ttcgggcgac tgagcccggg actggtcgag
     9661 gaacctcaga caatgcgtaa aaagacagtg gcggtggtgg gggcttcggc ggacccctcg
     9721 aaatatggga acaaagccgt ccgggcgcat gtaaaggcgg ggtacgacgt gtaccccgtc
     9781 aaccccaccg ccgagcttat cgaagggttg aaagtgtatc ggaacgtcct ggacattccg
     9841 gtggaactgg atcgtgtcag cgtgtatctc ccgccggaga agggggtccg cgtcctcgaa
     9901 gacatcgcct gcaagggcac gaaggaactg ttcatcaatc ccggcgcgga aagcccccgg
     9961 ctcatcgccc gggcccggac cttgggcctt gtcgtcatcc tcggttgcag catcctctcg
    10021 ttgggagaag atccgcagac tctctgattc tttccctccc ggcctccgtc ttccccgtcg
    10081 ctattttgct aaaatctcga tgccgctagg tatcgcccca gtttgtcgga tggggcgatg
    10141 ccgcggggtc ggtagacgcc gctgccgatg ccgcggggtc ggtagacgcc gctgccgatg
    10201 ccgccgggcg ttgcctccgg cttgcggaga gtggcagaca caacgatacc ggtttttctc
    10261 aacaccgtgc cgccccgttg cgccgccacg ggacaggcaa gcggcccaga gggccgacga
    10321 ctcttgccga ggtagctcag ctggtagagc aatcgccttg taagcgatag gtcgtgggtt
    10381 cgactcccat cctcggctga ttttttcaca tttcgtcgcg gtccttcgcg acaaggagga
    10441 tgaggatgaa acgactcgcg ctggcgctga tgttctgctt ggccggctgc atcacgtttc
    10501 cgacggatcc gcaatcggcg gttccggaac cggaaccggc tttcctgacg gccgaccgcg
    10561 acgcggcccc ggtcttggtg aacctgctgg acgagcgaaa gctcgaaagc cccgtgggcg
    10621 cggagttcag ttcgaccatt ctgaaggaca tcgttcggct gaccacgccg acaggatatc
    10681 gtctgaagaa ccgctatctc aaattggggg tcccgttggc gcaggcgctc tatttcgcca
    10741 aggacgccca gttgcagaac cggcttttgg aggccgcccg ctggaccagc agcccccggg
    10801 cccgttcgga agccctcgtc gtgttggcca agcagagaaa tccggaacat cttaaatatt
    10861 tccgcgaagc gctgttggac agcaacgtcg ccatccaatt cgccgccatc gaggcgcttc
    10921 aggtctgggc gggcgaaggg gcctccgaac ttctcctaag cgtggcgaac cagaattggt
    10981 ctccggtcat ccgggtgtac gccgcccaag ccgctctgcg gctgggttct tccgccggcc
    11041 gggacaagct cctggagttt ttgagggagc ggaactggct gatgcgggcc atggcggcac
    11101 gttacctggg agacatggga cggccggaag acggggatct cctgctttcg cgcatcgggc
    11161 cggaacagga caacaagttc gtcctggccg aggtctgcat cgcggcgctt aagctgacgg
    11221 gccggcggct gccggcgtcg atggctcctc ccccgcctcc gtcgccgcgc gcgcctctga
    11281 agcccactcg gcagcccttc gagttggagc ctctcgtcgt taccgcgccc cgcctgaaac
    11341 tcatgcagca cgtggacgtg cgcatcgaca acgacctcgt gaggcttctg gaaaaaatgg
    11401 cttccgaacc gatcgaagtg gaaaaagtcg ttgatcccga cgtggctcag ctttcccgca
    11461 tcgagacgcc ggtgggattc agcctggccg tgcgttacaa cgacctcatt ttcctcctca
    11521 tcgaaggcct ggccggttcc cgcgacctgg ggctggttcg tcgtctggag gagattgccc
    11581 gaaacaattc caacgccaac gtccgcgccg ccgccctggt ggcgctgggg tatgaccgcg
    11641 accgcaccga cttggccatc tttcaggcgg ctctgaggga tgccaccaac acctcggtcc
    11701 gtttcgcggc ggtggaggcg ttggggcaga tcggcggttc tcaggtgatc gggaccttgg
    11761 cggacgtggc caacagcgac cggtcccgcg ccgtgcagat cttcgccgcg caggcgctcc
    11821 gccgttccgg cgatccctac gggcgcgaaa tactgttgcg cgctctggac gacgccgatc
    11881 cctacgcccg cgccatggcc acgtattatc tgggagaagt gggcgaaccg gacgactata
    11941 accgcgtgct cttccggatg aacaacgaat ccaataattt cgccgtggcg gaggcctgcc
    12001 tggctctcta tcggctgtcg ccgtgaattt gaacctcgac tcaaaggaga catcatgact
    12061 gaattcggag cgttgaaagt tttggcgtat tcgggtgggg agtgggtcat ctacctcctc
    12121 ctcgcgtgct cgatcctgac gatcgccgtc atcatcgaaa gggctttggt tttgagacgc
    12181 gaacggcgtc aactgtgcgg tttggaaaaa gaactggcga cccttctgga gaaggggaac
    12241 ctggaatccg ccgccagcgt gtcggccggc gcccagggag ccgcggcccg actcctctcg
    12301 tcgggactcg gccatctcaa ggccggcccc gcggccgtgg aggaatacat cgcttcggcg
    12361 aagatcaccg agaaacaggc gatggaacgg cgcctgttga tcctgggcag tctcggcaac
    12421 aacgcgccgt tcatcggtct cttcggcacc gtgttgggcg tcatcaaggc gttccacgac
    12481 ctcgccgaga ccgccggcgc cggcccggaa gtggtgatgc aggggctttc cgaggcgctc
    12541 atcgccaccg cggtgggcct cctggtggcc atccccgcgg tgatggcgta caactatttc
    12601 cagaaacggg tgaacgattt cttcgccgac atcgaatccc tctcgaggct cctcctggcg
    12661 cgcctgcggg acgaatcgcc gtccaccaag tcctcgggcc gctgacccat ggccggtcct
    12721 gccgggaatt cggaaaactc gctcatcaca ggcattaaca tcacgcccct ggtggacgtg
    12781 gtgctggtgc tgctcatcat cttcatggcc acggcgccgc tcctcatgcg ccgcgccctg
    12841 tcggtgaacg tcccccgcgc cgcccacaac gaacccaagg ccaccgccac cctccagatc
    12901 cagatgacgg ccgtcggcga ggtgtccgtt gaaaaaaaga agttggacct cgaacagttg
    12961 gaccggatgt tgcgcgagcg gctgctgacc gaccccgccc tgcatgtttc actggccgcc
    13021 gataagacca tcccctacgg cgaggtggtg ggggtaatgg acgtggtgcg cggggccggc
    13081 gtcaaaaaat tggctttgga ggtgaagtca aaatagcgcc atgcccgcga tccccttcgg
    13141 cgcctttttg agacagcatc gtcttccgcc gttcgaaacg gcgttgttgg tgtctttttt
    13201 cctgcatgtg gccgcctggc aggcgctcac gtggaaggag cggtttgtgc gccaggaaga
    13261 ggtggaccgg ctggaaatag acctcacgcg cccgttccgc ttgacggaca atcctttcct
    13321 ggcgcgccgg gccaaggtgt cggggaccgg ggcgcccatc gtggaaaaac ccaaacccgt
    13381 gaccgcgccg cccgcggggg aagtcaaacc gccgagcgaa tgggtcctgc ccgggccgga
    13441 cacgaaggag ttggaaaagc cggtgacggc cgaagacccg agcgggctcg gcgggttcgg
    13501 ggtgggcgag ggcggcggcg aagtggactg ggtctacctc accagtctgc cgcagttgtt
    13561 gaaccgcgac gcgcttttga ggaacctgcg gcggtattat cccgaatcgg agcgccgcgc
    13621 cggacgcgag gggcaggtgg tgctcgacct gcacatcgac cgccagggga gcgtcacggc
    13681 cgtcgatgtg gtgacctccg ccggggcggc gttcgacgcc gccgccgaaa aggtgatccg
    13741 cctggcccgt ttttccccgg caaaagtgga aacccgcgcg gtgccggtga agatccgcca
    13801 gaccgtcgct tttcaactgg aagagtaaat cttccgcacg caaagagggt gtagtataat
    13861 ttggctcaac ggatttaacc catggccgag cggcgcacga tagaaatttc ctgcccctgc
    13921 tgtcaggcgg tcctcacggt ggacgccgag accggcgatg tcttgctcca caaggaagcc
    13981 aagaaaaaag tggagatgga catcgccaag gcggcccagg cgttgaagga cgacgtgtcc
    14041 cgccgggacg atattttccg aaaatccatc gaggcgcaga aaaggaaagg cggccgcctg
    14101 gacaagcagt ttgaagaagg cctccggcgc gccaaagagg agccggaaac cccgcgcctc
    14161 cgggagatag acttggactg attttttcgg cctccgcgaa cggaagattt tttccatccg
    14221 atccggcatt tccacacgac actggacccc gggagttatc gagtggcaca catcatcgca
    14281 atcgcgaacc aaaagggagg cgtcggcaag acgaccacgg cgatcaacct cgccgcgtcc
    14341 ctggcgcatt acggccacga gacgctcctg atcgacatgg atccccaggc caacctgtcg
    14401 tcgggcttgg gcctgacgca cgataaggtg accaaacaca tctaccacgt gcttttgcag
    14461 gacaccccgc tggaggagat tcttcgcgaa acgggcgtgg agtggctgga cgtggcgccg
    14521 tcccacttgg acctctacgg cgtggaggtg gagttggtca acgccgagca ccgggaacgc
    14581 cgcctgcaga tggccctgga aaaattccag aaggcgtaca agttcatctt catcgactgt
    14641 ccgccggcgc tgaacctcct cacgctcaac gcgctggtct cggccgccac cgtcctcatc
    14701 cccatgcagt gcgaatacta tgccatggag gggctgtccc tcctcattca gaccatcgag
    14761 cgcgtgcggg gaggactgaa cccgcgtttg gaacttgagg gggtgctgat gaccatgtac
    14821 gattcgcgga ccaacctcgt ccaacaggtg catcaggaga tcaaaaagtt tttcggcaac
    14881 aaggcgtaca agacggtcat cccccggaac atccgcctgg ccgaggcccc cagtcacggc
    14941 ctgccggcgt tgggctacga caagcactcc accggcgcgg aggcgtacct ggctttggcg
    15001 cgggagttcc tccgacggca cggcgccgat gcggaacacg tcgagaccgc gtcccccgaa
    15061 cccgtttcgg cggcgcccgc cggggcctga aggtttttaa aaggagaacg catggaacat
    15121 aaaccgttgg gacgtggatt ggaagcgctc ttaaaacaag ggacctcgac gctttccgcc
    15181 gcggaccggg agagcgtcgc caagatcgcg gtggacaaga tccgccccaa ccgctaccag
    15241 ccgcgcgtcc atttctcttc ggaatccctc caggacctgt ccgcttccat caaggcccac
    15301 ggtttggccc agccgctgtt ggtgtcaccg tcggcggtgc cgggcgagtt cgagctggtg
    15361 gccggggagc ggaggcttcg cgcctccaag atggcgggcc tatccgaggt tccttgcgtc
    15421 gtccgggcgg tcagcgagcg gcagagacac gagctttccc ttgtcgagaa catccaacgg
    15481 gaggacctca acgccgttga ggaggccgag gcgctgaaga agttgatgtc cgagttttcc
    15541 ctcacgcagg aggaggtggc gcacgccttg ggccgcagtc gttcggcggt ggccaataaa
    15601 ttgcgccttc tggagttgcc ggaggatatc cggcaggcca tcatggtcgg ccagctttcc
    15661 gaagggcatg cgcggaccct gctcggcatc gaggaacgaa gccgccagac cgaattgagc
    15721 cgccgtatct tgcgcgaaca cctcaccgtg cgcgacgtcg aaaaaatcgt cgctgattgg
    15781 cagtccgcgc accacgaagg ccgcgtcaaa acgacgaaac gaaaagaccc cgacgtccgc
    15841 cagttggagg aggacctgca gagaacgctg ggccgcaagg tggagataca gacccggggc
    15901 aagaagaaag ggtggctccg gctcgctttc tattcactgg acgatctgca gctgctcatc
    15961 cgccacttga agaaatccaa agacgggcat tccggccgcg gcgcatgaac agggtctgcc
    16021 ttttttgcgg agtgtttctc ctcggcggct tttcttgggc cggcggaagg aaaccggaac
    16081 ggatctccga aaaatccgcc gcccccgctg ctccggcggc gacgacaccg cctccgccga
    16141 cgttgatgcc gtcggccccg gcggagacgc cgcttttctc ggtgcccgac gacaaaagcc
    16201 ccgtctatct cacggagatc tccaacccca atgattttgt cctcttcgcc aacggcgggt
    16261 gggacgggaa ttggtacgtg ggttacaaca cctgttgggt ctcaaagttg ccgccggcgc
    16321 ctcccggcga atataagagc gcttttctgg gggcccgtct gggtcggatg aaaaccgagt
    16381 ccgttcccgg ccgcccgccg tgggaaaaaa gacccatcaa ggggttcatc aacatggctg
    16441 ttgccgatga gccgctttgg ccgcagagcc gccggtttcg cctgacggaa accgccgaca
    16501 tcccgctgga aggggacgcc gaaaacgccg tggagggggt gggggagtcc cgttggtttt
    16561 gggtggaggt gccgctgaag ttcatctctt tcgaagagga taattatgtg gcgctcttct
    16621 cccccagcga ggcgctctcc tcctcggccc gcgcccccgt gctggccgcc gcctggggag
    16681 acacccgtca gaacacctgg ctcaattcca ccgtcaaggg agagccgccg ttcacctcca
    16741 cggacgcctt gaagacaccc gtcacctatt tcgagccggc catcgccatc aagctggttc
    16801 ccgtcaacga ccgtcggccg gacatccgcc tcttggaaac accggaggag ggcgcgacgg
    16861 tccaggagaa gatttttgtc tccgcgtccg tttccggccg ggacatggcc ggggtctggg
    16921 tcgaattttc aacggacacc aagaattggc gccgggcggg gagcttcctg gcgatcgctc
    16981 cttacgtgtt cacggtcaag cgggaacaat tattggaagg gacggttcgg ctccgtgtcg
    17041 ccgccgagga tgtcatgggc aacagggccc agagtcccat tttttccgtc cgggtcccgc
    17101 ccccgccctc cgaacctccc aagaagagac gctgactttt tacgggtcct ttccggtgaa
    17161 gccgacgata tccgcttctc tccgtgcgcc ggtcttcgcc gtcggtcttt tcctcgcggt
    17221 tgccgccggc gccggagaat cgctccccac gcagagttac cgcttcctgg actggaccgt
    17281 gcctcaccgc gtggaggagg cctacatcca gtacgtctac gacggcgata ccgttcagga
    17341 ccgctaccag cgccgcatcc gcctgctcgg cgtcgacgcg cccgaggtgg cccatccgga
    17401 acacgataaa ttcaccaacg aacccggcgg catcgaggcg ttgcacttta ccgaaaacaa
    17461 ggtccggggg aaaaaggcct atctggccat cgacgatgaa aacgccgtcg ataaatacgg
    17521 ccgcaccctg gccgtggtgt tcgtgaagga tgatttgggg gagtggtcct gcctgaactg
    17581 ggacctggtg cgggagggga tggcggaggt gatgatcctt cccgggaatc gtttctgcgt
    17641 ggaggccgaa tggcacaaac tcagcgccgt ggccagccgc cgccgcgccg aggatttccg
    17701 ggagctcgcc cagtcgttca aggacgaggg gcgttcggaa agcgccgtgg agacgtatca
    17761 gagggggatc cgccttttcc ccgatgcccg ggcgctttac gaggacctgg ccggcctgta
    17821 tctggggatg aaccttcccg ggttctccgt ggatatttac ctggcctatc tggaacgcca
    17881 tcccggggac gtcctcatcc gttatcgttt ggcgcgggcc tatgagctga tggccgccgt
    17941 gcagtccctg ccgctggtgg attaccggaa aaaatccacg gaggagtggg gccggctcct
    18001 cggcaccgaa tacgacgccg aggcaaggca gacgcttttg cgactggaag ggaaaccctg
    18061 aagttttttc attacaagga ggaaccatgc cgcgtccgaa tttcctgaag cgaaaatatc
    18121 ttgtcaataa gccgctgcag ctgaaatacg ccttctggat cggcgccgcg ctgtttgtcc
    18181 tcatgctgct ggcgcaggcg cacacctact tcaccatcga atccatactg ccgaacctct
    18241 tttcgagcgc cctgggccgc caggcgaggt ccctgcagac gtggcttctg ctgaacagtt
    18301 tgatctacct ggcggtggtg gtgatcctgt ccgttttcct gtctcaccgc atcgccggtc
    18361 cggtgtatca tttcgaaaaa gagatacggg aggttttgga gtccaacgat ccttcccgcc
    18421 ggatcactct ccgcaagggg gatgaacttc attctttggc ggaccagatc aacaagcttt
    18481 tagagcgcct ggcgcagagc cagagacgct gaagaggagg cgaccatgaa acgtttatcc
    18541 cttattattt ttttcctcgt tgtcctgggc tgttcgaaat ctcctgaagg cgtctccaat
    18601 accgccacgc gctacgtgga cggtcttcaa gccgatgtgg agaaggcgaa agacgccgcg
    18661 gccgccgcca ataaggtcat cgcggatacg caggccatga tgaaaaaagc cggcgaggcc
    18721 gtcgaatagg cgcacggaaa gaaaagtttc agcgggattt gtgagagtcc tccacgtcac
    18781 cgaaagccgc agttggagcg gcgggacggt ccagctttgg cacctgtgcc aggccctggc
    18841 ccggcacggt cacgcggcgg cgctgttttg cccaccggag agcgaactgc tcaggcacgc
    18901 cgccggatcc gacgtggccg tcaccgtctg ccccatgcga caagattacg acgtcttcgc
    18961 cgcccgcgcc ctggccgagg ccatccgccg ttttcggccg gacatcgtcc atgcgcatca
    19021 tccccgggcc cacgcgttgg cgcttctggc gggctttttc gtcgacatcc cacggctcgt
    19081 tgtttcacgc cgggtctctt tccgtctcaa aaaatggaat tttttcagcc aatggaagta
    19141 ccgcacctcc cgcatccgcc tcttcgtggc ggtctccgac gacatcaggc gtgtgctggt
    19201 ggcggggggt gtgcccgaag aacgcgtgga ggtgatccag agcggggtgg acgtggcgcg
    19261 tttttcgccc cgccctccgg acgagaagtt gcggacggaa ctggcgttcc cccgggggac
    19321 gcccatcgtc ggcaatctga cccatttcag ttggtggaag gggcagacct ttttcctgga
    19381 ggcggcccgc ctcgtccttt cttccggcgt gccggcccat ttcctgcttg ttggaaaaga
    19441 cacggatggc cccgcggcgc gggccaaggt gaccgaactc ggcatcgaga aaaacgtcac
    19501 gttggccggg tttcggacgg acatgcccga ggtgttgtcc ctgctcggcg tgagcgtggt
    19561 gtcgtccttg gccggggaag gattttcggg tgttcttcgc gaatccatga gcatggggat
    19621 ccccgttgtg gccaccgacg tgggggggaa caaggaactc gtgctcaacg aaaagaccgg
    19681 acttctcgtg ccgccgggtg acgcgcggtc tctggccgat gccgtccggc gtttgctgac
    19741 ggataaaacg ttggccgatt cttgcgcgcg ggccggacag gagtcggtgc ggtcccgttt
    19801 ttccatcggg aaaaccctgg agcggaccga aaaactctat gaacggcttc tggcggaacg
    19861 ataaaatgag tttttttaaa aaaacgctcg tggccgcgtg ggtgatggtt cccgggatcg
    19921 ccgtggccgg ggaaatatcg ctcatgtatc tccctcagtc ccagatcgaa tacgaaggca
    19981 cgggctatcg ccgtttcgac gaagggtggg aattgaaagt gaaaccgcag agcgcggcgg
    20041 cgggatttct ttggaggcat ccgttgtcgg atcgatggga tctgcaggcg cagttctggc
    20101 ggaatcagtc cttctttgcg cgagaggacg gaaacgccaa aagcgatctc atgcgtcaga
    20161 cgggccaaac ggaattgacg gtgaacaacc tcatccttga tgtccgtcgg ccgttggcgg
    20221 gctcttccgt ccgtgccgtc gccggcctgc agggggtcta cgagaatttt caccggaaag
    20281 agatcctctt caacgccgtt ccggaggcgg gggattcccg ggaaaatctt tcggccgccg
    20341 gcgccgtgct ggggttcgcg ggggcttcgc ccgattcgac ggacaggaac cgattttatt
    20401 gcgggtggga agcccttttc ggccattttt tctatacacg cacgtcccac gcgatcgacg
    20461 gcggctccat ccaccgcgac ggttattctt acgctctgcg tttagaaacc ggatatcaga
    20521 tgaggaaatg gcggctgggc gcgggatttg tgcggcaatt gatccaggtg caggttcccg
    20581 gcggccggac gttccccggc ggcgccgcgg cgtctctccc catcaacaag acggatttct
    20641 tcggtctttt cctggcgttg tcccatgtgt attagaagat tgttgaaacc ggccgcgttc
    20701 ttgtgggtga tcctcgcctt gttgcccgtc cgggcgtcgg cggacgacgg ccttcgctgg
    20761 aacctgacgg tccttcccca cgcctccctc cgataccgtg gggaaggaaa ggtcctgccg
    20821 gagagttacg tcaatcggtt tgccggcgaa acgtcaccga tataccggtt tgaactgaca
    20881 caggtccccc gctcggacgg ctattggggc gtgagtttct ggcacacggg ggttttcgga
    20941 gggggctctt atgccgagga gcgcgtgccg gacaacacca ccggcggcgc ctatcagatc
    21001 aaccagctca acgtgggatt taccaacctg ttcgtcacct accaccgtcc cgtggcggct
    21061 ctgccggtgg aggcccgggt gcatgcctcg gtggtgaggg agatatttaa aaggaaacag
    21121 ttcatcgttc agaacgtgga ttatcgctgg acgaacttcg acgacgtcaa tgagatctcc
    21181 gccgaagggc tgggtttcgg tttggccggt cggcacgggg gacgattcta tctgcgttgg
    21241 caggccgacg cccactacta catacaactc ttcgatgcca agaccgattc ttcggcgggg
    21301 cagatttatc agtcggaggc cgccctcggg gcccgcgtcc tgccccgcct gtccgtggaa
    21361 ttcggcgcct tgtggcagta ctggttcacg cattctcagg ggaaccgccg gctggtcatc
    21421 cccggcacgg acggcgcggt catttcatgg aaccggcagg agacgcgcgc gtccggcgcc
    21481 tttttgcggt tggactattt ttttgcgggg cgggactgac gacgtcggtg tcgaatggga
    21541 tggtctaaaa ttcgaatttc ggagcgtacg acgatgaaaa agaaaccaat aaagtatgca
    21601 cgacgcgggt tcttcgccgc gttgacggtt tttgcggcgg ttcttgtcgc ggaggaaccc
    21661 ggcaagcccg cgtcggcttc gggaccggtc tatgttttga acatcgacgg gaccatcgac
    21721 ccggtcatcg ccaaatacgt ggcggatgct tttgcggagg caaaagagga aaatgccgcg
    21781 gccgttgtga tccggctgga caccccgggc ggactcttgg acgccacccg cgacatcatc
    21841 cagcagatat tgaacgcgcc gatgccggcg atcgtgtacg tgtccccgcg gggctcgcgg
    21901 gcggcctccg ccggggtgtt catcaccgtc gcctcggacg tggccgccat ggctccggaa
    21961 acccacctgg gggcggctca tcccgtgtct ctcggaggca atccgttcat gccggcgaaa
    22021 cccgccgatc cggagaaaaa tgggaaagaa gacgagggaa gatccgtcat ggaggagaag
    22081 atcgtttccg ataccgccgc ctacgcccgc accctggctt ccgccaaggg ccgcaacgcg
    22141 gattgggtgg aaaaagcggt gagggaaagt ctttccctta cgtcaacgga ggcgctggag
    22201 aaaaacgtgg tggatatgtt ggcggaggac gagacggacc tctttaaaaa gctctcgccc
    22261 cgggatataa agaagaacga gaaggtcttc cgcattgatt tcgagaaggc aaggatggtc
    22321 cggttcgaga tgggccgcct acggcaggtt ctgcacgtgt tggccaaccc caacgtggcc
    22381 tacctccttc tgatgctggg tttttatgcg ctcatctacg agttcgccac gccgggcatc
    22441 gggttgggcg ccgcggcggg gctgatcagt ctggtgatgg ccttttttgc cctccaggtg
    22501 cttccgctca gctacgcggg gctggcgctc ttgttggcgg ggatgacgct catggccatg
    22561 gagctgttcg tcgtcagtca cgggctgttg atggtcggcg gcacgctctg tttcatcgcg
    22621 ggttccctgt tgctgttcga atccccggag ccgtatttcc gggtttcgct ggaaatcatc
    22681 ggcggcacgg cggtggcttc tctggcgttc cttgtcttcg tggtcaagaa aatactggcc
    22741 gtgcgcgtca tggcgtccat cacaggcaag gaggcgctcg tcggccaaat cggggaggcc
    22801 cgtccggacg gcatggtttt tgtgcacggg gaatactggg aagcggaatc cgccgcgcct
    22861 ctttcgccgg gacaaaaagt ccgggtggtc gaagtcatgg ggaaccggct cagggtggaa
    22921 aaagtatagg cggcgcaagc ggtttgggcc ccttgatgga tttttcaggg agccgcccgg
    22981 aaacaaaaag attttcagag aaagtctaat accggaggtt ttaaaatgat gggaattatg
    23041 ccgatcatta tcatcgcagc gatcatcatc atcaacgccg tcaaaatcgt gcaggaatac
    23101 gagaggggcg tcatttttcg tttgggccgt gtcgtcgggg ccaaggggcc ggggctcttt
    23161 ttcatcattc ccatcgtgga ccgcatggtg cgcgtggggc tgcgaaccgt gaccctggac
    23221 gtgcccgtgc aggaagtcat caccaaggac aacgtcccct gcaaggtgaa cgcggtgtgt
    23281 tacttccgcg tgctcgaccc cgtcaagtcg gtcatcgagg tgcaggacta catcatcgcc
    23341 acgtctcaaa tcgcgcaaac aacgctccgc agcgtgctcg ggcaggcgga gttcgatgag
    23401 ctcctcagcc agcgggagaa aatcaacacc aaactccagc acatcattga ccagcagacc
    23461 gatccgtggg gcatcaaggt gagcatcgtg gaggtgaagg acgtgcaggt gccggaacag
    23521 atgcagcggg ccatcgccca ccaggcggag gccgaacgcg accggcgagc caagatcatc
    23581 gccgccgaag ccgaatacca ggccgcccaa aaactggcgg acgccgccca gatcatctct
    23641 tcccagccgg tgtccttgca gttacggttc ctgcagacgg cgctggagat atcgaatcag
    23701 aaaaattcca cgctgatctt cccgttgccc atcgaactct tcaagagttt tggagcgtat
    23761 ctggaaaaag ccaggaattg atccacatcg tttccgagca acaaaaggcc gcccttcccg
    23821 gggcggcttt ttgttgggcc gacagggatg gtttaattgg acagcggttg gtttttgaag
    23881 gaaagcgccg gggcggagat ggtttctccg tcccagagtt ccagggaggc gagggaatcg
    23941 gccaccacgg cgtcgaggtt gatctttttt tccagttcaa gggccttgtg gaccggcatc
    24001 atggtcttgg gatgccgcga catcagggaa cagcagtcct tgtagggctg gatggagagg
    24061 tcgtaggtgc cgattttttg cgccaggcgg acgatgtcgt ccttgtcctg cgagatgagg
    24121 gggaggaaaa cggggatctt caggtcggcc tgggccgccg cgatgttctc cagcgtttga
    24181 ctggccacct ggccgagact gtctccggtg accagcgcct tgcaccgtcg acgttcggcc
    24241 agtttttcgg cggtcttgaa catgaatcgg cgaaaaagga ccatctcgta catgctgggc
    24301 acgtccaacg cggctttcag ctgaaaatgg tagtgcggaa tgaggtagag gcgggagccg
    24361 gggctgtaga gaagcagttg cttgtgcaga gagaaaattt tggtgtcgcg cagttcttcc
    24421 gcccggcgga acgggtggaa atgcagcagg tgcaccatcg cgccccgccg agccagcaac
    24481 caggccgcga cgggggaatc gatcccgccg gaaaaaaggc agagcactcg gccggagctg
    24541 ccctgcggca tgccgcgcag cccgggcttc cgttcgctgt agacgaaaac ctcgtcggct
    24601 aaaatctcca cgtagatggc caggtcgtgc cgctccaggt ccactttgag gccgtatttt
    24661 tccaccaccg ctcggcccag gcggttgcat atttcctggg aggagagcgg gaaagttttg
    24721 tcggcccggt gggagtagac cttgaaggcg cgcgcggccg ggtgggccgc ggcgcaatcc
    24781 agtcccgcct gtaaaatggg ttcccattcg cgcggcacgc tttgggccaa ggcataccac
    24841 gccacaccga acacctgatt caagtgggat tccagccgcc ctttttcgaa ccccggggac
    24901 aggcgcacca cgacccgtcc cgattccacg cgaacgcctt cggcgtccgg ccccagccgt
    24961 ttttcgatat cgttgcggag ccgtcgttcg aaaaacgaac ggttcttccc cttggtgccg
    25021 atttcggagt agtgaaccag caaaatgtcg ttcatgtcag ccatgataca aaaaaagtcc
    25081 gtcggccgcc ggtttgtcga cgacgggcat tttcgcgggg agcgagaggg cggttatttg
    25141 cggttgtcgg cgggccggag ccgttggagc ccggaaaaga gcccgtccca gtcgttggcg
    25201 gaaatccaaa agaggctggg ttcctcgttg cgacggagcg gatatcggtt ttgaacggcg
    25261 tctttttcgc cggcgtcgag gcggacggtt tttccggaac taaaaaggac ttccaggctg
    25321 ttcttagtcg tcaacaaacc gaattttttt gtgtcggagg aggccggggg atcgatgaat
    25381 tcctccacgc tcaggacgcg cagcgaggca aggaaatttt caaccgccga cgtttccgcg
    25441 gtcttcccgg cgagggtcca ggtgtccgag gcgcggagca gttccagagc ggttttctcc
    25501 cggcggaaca ccagtcggtg gatgccgtcg gcggggtccg tcttgaagag gcgccgttcg
    25561 cgccagcggg cgagcggctg ggtgagcgcg tcccgctcca cgcctttggc cagatacacc
    25621 tccggccgcc ccggccggcg gaggtagatg tggaggaagt ccggggccgt tttcccgaag
    25681 agccattcgg ccggctcttt tccttgcgga ccccaaacgc gcaccagaat ggcgctggaa
    25741 tcgttcactt catagagaga atgcgtctcc gcgcggtcgg tgaggcgttc gtccgtcgac
    25801 agggaggaga gggaggaaaa aagaagcttc agcgtttcgg cgtccgccgg atagtccaga
    25861 ggttgttgca gggtccactc gtcgccggac ttctggaaaa gaagacgcgc gtcggggcgg
    25921 acgatctcca atttttccgg agatgccggc aattcgggaa ggggcggttt gggcgcgggg
    25981 cgggggattt taacggcgat cgttgccgcc gtgacgagaa ggagaatgcc gagaagccgt
    26041 ttcagccagg agggaagcat gtcaggtcgc ggggttccgt tgaaagaggg accggacccg
    26101 ctcgcgttgt ttccgccggt tctgccagcg ccacagcccg cccgccaaga ccacgacagg
    26161 aagcagaaag tagcccgcca gtttgaatcc ctgtttggcg gggtcggaca gggggcggat
    26221 ggggcggaac ggagagtttt tcgacggaat ggagagaaag ttcggatcct gggcgagcca
    26281 ttcgctgaga ctcgccaggg tggtcaggtt ggtttccggc acgggcatgt ggggggcggc
    26341 gtaaaaggcc gttccgaag
//
LOCUS       DAOTFD010000016        26259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1215_length_26259_cov_5.160853, whole genome shotgun sequence.
ACCESSION   DAOTFD010000016 DAOTFD010000000
VERSION     DAOTFD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 26259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26259
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_26259_cov_5.160853"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="P5079_02055"
     CDS             complement(<1..1240)
                     /locus_tag="P5079_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HRY28802.1"
                     /translation="MNTSLHYADVLKQLKEYESRNEERPVIRWSFLSNVTLDMLFPYV
                     KLLCFEAGLRPQIAVGGYDTALQDASNGKSGLYAAPPDVCVVALRLESLAPALFRGFG
                     GLSPQQRQEETSRVLQYVVSVVEAIRRHSAAPILVHGFESPLYPALGILDFQSLDGAA
                     NTVRALNLELIKTLKRFRDAYVVDVESVRARVGQKEFFDARYWHIGRAPYAPHALEEF
                     AAEYMKFVRACRGRVRKCLVLDCDNTLWGGVAGEDGPGGVLIGKTFPGSAYHEFQEAV
                     RELARRGVLLALCSRNNPQDVQEVFAAHADMPLRWDDFVSRRINWNDKVSNLREIAKE
                     LNIGLDSLVFMDDSEFEVAMVNKFLPEVAAVRLPEEATSYRGLLESCGLFDSLEFTDE
                     DRRRNEMYAAEQKRQTVRSGA"
     gene            complement(1237..1656)
                     /locus_tag="P5079_02060"
     CDS             complement(1237..1656)
                     /locus_tag="P5079_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY28803.1"
                     /translation="MIIDHIGIVVESIEEASVYYARHFGYAAEGAPVTDPLQEAEVLL
                     MRDEKGQAIELIRPTSPTSPISRAMKKGGGLNHVCYRVKDLAAASAHLEQLGAVSVRS
                     ARPAVLFGGRRVAFYFTRANEIVELVEEQSPGTGGGK"
     gene            complement(1653..2561)
                     /locus_tag="P5079_02065"
     CDS             complement(1653..2561)
                     /locus_tag="P5079_02065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY28804.1"
                     /translation="MGRIEEAPFWEHKGIRVVFDPEWRCRRQEPLPTKAWIKTFYEKR
                     YFQEVKDGWDELQNEEREFWERIFEDKRVNLEELLPGGSAPWKCLDVGCGNGMFLAYL
                     QRRGWKVRGIEPSPLARLARDKYGVECSPDFIENVPVPSAEDKFHVVHVADLLEHVLE
                     PYELLRKCHAFLRPDGIFLLDHGLDFNPFQLSAVEQMGLPCWWIAPEHITYWTHDQVV
                     GALQREGFEIVRKESDFPIDLLLLMGIDYVSHPERGREAHRMRENFERVLSKTGRDDV
                     RRRFYRQLASVGLGRQIRFYCRPGKT"
     gene            complement(2576..3682)
                     /locus_tag="P5079_02070"
     CDS             complement(2576..3682)
                     /locus_tag="P5079_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRY28805.1"
                     /translation="MRYCTRCIMPDTRPRIEFDREGVCNACRHADEKKKIDWEARRRE
                     FLSLVEPYRSKDGAWDCIVPWSGGKDSSSIAYRLKFEYGMNPLLVTFSPQLPNAVGMN
                     NREALIRCGFDHLFFRPDQKIHRHLARRFFRERGNQKVAWDAGVNTVPVQVACHYNIP
                     LVFYAEHGESEYGGKVLREESKKVRDFTEVLEHQVGDDPRNWIDDVVGVKDLNPYLYP
                     SAEEVKRVGVKALYFGYFFRWSMLNNYNFIKDRIPFQTCPKGRTDGTFTDFDSLDDKS
                     DNIYYYMQFVKFGFGRATRDACRMIQNGQMTRGEGLEKARKYDAEFPRDFFPEMLDYL
                     GIEEEEFHRIVDQHRNGEIWKKEGNGWALRYPPK"
     gene            complement(3714..4463)
                     /locus_tag="P5079_02075"
     CDS             complement(3714..4463)
                     /locus_tag="P5079_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HRY28806.1"
                     /translation="MLRKRLVTVLTFNDGVLFRTKEFQPDYRYTLNFVDAWAVDEIVV
                     LDVTRPGTGDRENFYGVVELFSERCFVPLSAGGGVRNIEDFRRLLNLGADKVVINTAA
                     LERPEFISEAAALYGAQCVVVSMDAKKKERGDYEVYSHFGARPTGQSPAGWAKRAEEL
                     GAGEIFVTAIDRDGSLEGYDNELNRLVSEAVKIPVLVCGGAGNWQHFVDGFVQGKAAA
                     VCTACIYHFTDTSIRSAKKYLATAGIPVRTA"
     gene            complement(4456..5082)
                     /gene="hisH"
                     /locus_tag="P5079_02080"
     CDS             complement(4456..5082)
                     /gene="hisH"
                     /locus_tag="P5079_02080"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRY28807.1"
                     /translation="MGTGTVAIVDYGLGNLRSVAGAVKRAGFALRITCRTEELEKADK
                     LILPGVGAFGDGMKNLRERGLVETLTELVIRRKKPILGICLGCQLLARESEEFGRHAG
                     LGWLAGAVVRLRPADPALRVPHVGWDDLVQKRESILFRGIAPDALFYYVHSYYVQPDD
                     PSLVYGECDYGMKFPAVLQRDNIYATQFHPEKSQRQGLDLLKNFLDNA"
     gene            complement(5091..6923)
                     /gene="asnB"
                     /locus_tag="P5079_02085"
     CDS             complement(5091..6923)
                     /gene="asnB"
                     /locus_tag="P5079_02085"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY28808.1"
                     /translation="MCGIAGYIGTAVLDDRRLDGCLALMRRRGPDHAARKDWTMRSGR
                     RIHFLHSRLNIIDLEARSNQPFQVGKKWLVYNGELYNYTEIRRELEAEGEVFSTTSDT
                     EVFLRGIKVFGWEVLDRYEGMWAFAVYDEEEDALVLCRDRFGEKPLYVHRDASGIYFG
                     SETKFIAALLGRRLEIDHEQMKRFLVNGYKSLYKEGQTFFKGLEELPAAHVWRVEGSG
                     RVQQKRYWKPSIGADAGMTFEQAVSGVRERLFRAVEIRLRADVPLAFCMSGGVDSNAL
                     IGIAKKELGYDVHGFTIANTDRRYEEQEMVDCAVKSLGLRHTSIPVDTRDFLANMRTL
                     VRQHDAPVFTISYYAHWLLMKSVADQRYRISVSGTGADELFSGYYDHHLAYLYDVRED
                     RALHGAALEAWEKNVRPWVRNPFLGDPDLFVRDPNFRKHIYLEADDFAGYLTEKWAEP
                     FREETLTQGGLLRNRMLNELFYEAVPVILHEDDLNAMYFSVENRSPFLDRALFEYCGR
                     IPVRHLVRNGRAKAVLREALRGIVPDPILDNPRKVGFNAPIRSFLDTADPRVKPALLK
                     DSPVFDWVRREKIEVLLDKKELPNSESKFLFYFLSTKLFLEEFS"
     gene            complement(6938..7948)
                     /locus_tag="P5079_02090"
     CDS             complement(6938..7948)
                     /locus_tag="P5079_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRY28809.1"
                     /translation="MKIGRFDTTEKVLVVAEIGNNHEGRFEVAEQLVRRAAECGADAV
                     KFQTYKTEKFVSARDAARFQRLKSFELTQPQFEKLAALAHSLNLLFISTPLDMDSAEF
                     LAPIVDAFKVASGDNTFYPLIEKVVLTGKPVIVSAGLTDYGEILETVKFMGRCRKKGG
                     VSGETAVLHCVSLYPTPPDQAGLRAIRFLTEKMDCAVGYSDHTTGLEAVVAAVALGAR
                     IVEKHFTLDKNFSDFRDHKISADPGELAELVRRVRTVSLMLGELEKKARPEERRMAPS
                     VRRSIAASRDLPAGHRLTREDLMWIRPGEGLPPGQEKRLLGKKLKRTVRPGEFLSPID
                     VG"
     gene            complement(7945..8691)
                     /locus_tag="P5079_02095"
     CDS             complement(7945..8691)
                     /locus_tag="P5079_02095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY28810.1"
                     /translation="MELEQDAWLSGILQKAAFRVSGAANGTRSHCDLVKKMRVVAKRG
                     PAFFYTRASTRDIASLRRFSEAGFYVVETNVLMERRPAARLPEFFGGRCRVRPVVAKD
                     ADRLLDIAGTSFEYSRFHLDPEIPRRVADRIKREWIQSYIDGKRGDELLVAEKKGKAV
                     GFLAVLAKREGRLSLRIIDLLAVSSRMRGHGVGRSLVGALVRGGAGRCHRLRVGTQAA
                     NGPALRFYENCGFRVKDTAYVMHAHSKRMK"
     gene            complement(8709..9722)
                     /locus_tag="P5079_02100"
     CDS             complement(8709..9722)
                     /locus_tag="P5079_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY28811.1"
                     /translation="MTDELKGKKILLIGGAGFIGHNMALTFSQWGAEVHIVDGLQVNN
                     LLWFSSQDNDSEHKDLYMRFIQERLDLLRKARIPLYVQDVRDYHALCRIMSQIRPQIV
                     IHLAAVAHAGRSNKDPYSTFDHSLRTLENALDASRGIVEHFVYFSSSMAYGNFLTPEV
                     DEDQPLNPIGIYGALKVAGEKIVIAYQQVFDIPYTIVRPSALYGPRCVSRRVGQIFIE
                     NALQGKPLRMEGDGKERLDFTHIDDLVEGIRVILTNPNSRNQTFNVTYGQSRSINELA
                     GIVTQHFPGSKIENVARDKLMPFRGTLSIKKAQKLLGYAPQNPIEVGFSKYIAWYKDI
                     HKG"
     gene            complement(9719..10879)
                     /locus_tag="P5079_02105"
     CDS             complement(9719..10879)
                     /locus_tag="P5079_02105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY28812.1"
                     /translation="MTAQRTIPFGRPWIGDEERRAVEEVLRGDVLAHGPQGKAFEAEF
                     AAVLGEGAHVVSVSSGMAALHLSYYQMGLGPGDEVLVPALTHTATVHAVEIMGARPVF
                     VDCEPRSGNVDVSKMESLITPRTKAIGIVHFLGVPCPMEEIVSLAERHGLHVVEDCAL
                     ALGTRLKGKHAGLFGDSGCFSFYPVKHITTGEGGMFVTRHKSVAGKVSKARAFGVDRS
                     FSERTIPGMYDVLSLGLNYRMSDINAALGRVQLTRLPEILRRRRANFAFLKDALKGSG
                     NISVLDGGATGEESSCYCLSVVLEGPLGAHRAKAVFKLNEAGIGTSVYYPQPVPRMTY
                     YREKYGYRPEDYRQAARISDQSIALPVGPHLTADDLDYIVTHFKRVIKEVLP"
     gene            complement(10876..11886)
                     /locus_tag="P5079_02110"
     CDS             complement(10876..11886)
                     /locus_tag="P5079_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY28813.1"
                     /translation="MKKTLRVGIIGLGVGERHLAAYRKLRACSVVALCDRAKAKRAWA
                     EKKYPKVRVSSRADELLNDKDVDLVSIASFDDDHFEQVLKALRAGKHVFVEKPLCRSR
                     AELRAVKREWTKSRGRLELGSNLVLRAAPLYRWLKKAIAAGEFGRIYAVDGEYLYGRL
                     EKITAGWRRRVKGYSVMLGGGIHLIDLFLWLTGDRPEKVLSTGNSICTEGTPFRYNDY
                     VAATFRTASGLVGRITANFGCVHPHQHVLRIFGTKKTFIYDDAGPRLFSSRSPEERPL
                     RIALSPHPKSKGDLIPEFVSAVLKGGGRRFPAQSFFDGISVAAAFDESLKSKTWEKVR
                     YV"
     gene            complement(11888..12589)
                     /locus_tag="P5079_02115"
     CDS             complement(11888..12589)
                     /locus_tag="P5079_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRY28814.1"
                     /translation="MKIAVVVQARMTSQRLPGKVLKVLGGGPLLARVWERVRRATTVD
                     EALVATSDDRTDDALADFCQKEHIGFFRGSLTDVAGRFKGVFEKYGLDAFVRISGDSP
                     FIDPALIDDCVRVYRKGDHDIVTDVLKRTFPRGQSVEVVGREAFFRAYPEMRTEEDRE
                     HVTPYFYRSLGAFRIFNLESGGDFGAARLTVDTPQDMLLAERMMTMMKKPHWEYGWRE
                     LLELQRAAAAFEGRA"
     gene            complement(12605..13087)
                     /locus_tag="P5079_02120"
     CDS             complement(12605..13087)
                     /locus_tag="P5079_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28815.1"
                     /translation="MTPTSPQDEFVRFAGQLAESFSFNKSIGQIYGLLYLNPEPLSLD
                     EISKRLLMSKGNASINLRVLEEWGAVRSVPIPGSRRDHYEANRNLKEVALRRLEEGLG
                     RRISWAEKRLDDFLTRTKESPGSEMNAETKKQLQEIRVLLARGRKAMALLPKIGRFLT
                     "
     gene            complement(13274..14293)
                     /locus_tag="P5079_02125"
     CDS             complement(13274..14293)
                     /locus_tag="P5079_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HRY28816.1"
                     /translation="MIITKTPFRISFFGGGTDYPTWYRENGGAVLSTSIDKYAYITCR
                     YLPPFFEYKYRISYTLNEHVKEIGEIQHPSVRGCLNYLKIQPGLEIHYDGDLPARTGL
                     GSSSTFTVGLLKSLYALTGKVVGNLELGHEAIHIEQNVIRENVGCQDQLAAAVGGLNH
                     MEFGPDDRILVTPLIVSKERMNQLQSHLMLVFTGLSRVGVKLAGDIIQNTPKKKEELR
                     AIQQMVKEALKILNSSADLTAFGKLLHESWNIKKTLADGITTPLVDDLYGAALKAGAV
                     GGKLLGAGGGGFVLLFAPPERHGQIRESLKKFLHIPFEFENFGSQIVYYKPEDSLRWQ
                     FPAGR"
     gene            complement(14343..16652)
                     /locus_tag="P5079_02130"
     CDS             complement(14343..16652)
                     /locus_tag="P5079_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28817.1"
                     /translation="MPPSFKIFFDSLRRTPAVAYFFLMAAVFYADKFLGPYGLIRLHD
                     TFDVEFARYKAAGELLFRHGFFAWYPVAAGGSPAYAHTSPPYHPLCLLSQFFPLWFLY
                     GALTITLMAVAGYGMYRFLNELLELDRPLASIGGFVFAVGTQIQNESIVHVVYNYAFP
                     LFFVWTAGAILKGRRISWGVSLGVLATVFFSYAALTIPFFSLLHLVLAWYVAGNDVYK
                     RKGLLWQWALLWGGYILVFAPQLYALYDYSLMTSRIYSPNVRGFFSYLAEFAVSLPVK
                     GLVVLPLAVSLVWSRSARVRHWLSLGAFFLAVSAFFYSSASNFLAGTFLPKMDLGHYF
                     WTLGFIATVTTFVALAHSRGGLGGGRGILLVLVAVLGVSWFELSPDERLFLIPANLLM
                     FGGLFFFVRRSAGEGTGLGRPLAIALFVTAALFARAYRVVAQEHHSYERDFGNAELLK
                     SALARSAAGRIGSWQLPPAPVMSVGLETLDGRGPFFYGPYKQFIGKAIEGQFQTAEQA
                     KEFYEYWYNLYLANGDLRRGPTDGLLQKINLPMLKAAHLTHLVSPGALPEMETVSKEV
                     TVIKDEKKWFRFLPGPLVELWRPVPLYVYALKDPQERAYLAEAAVILPTGKEVLSQMA
                     SSPEKLKDTVFFSQEDASAPSAAWAAGAFVGKKKLKLERAGPDELVFEAETEGPAVLV
                     VSHNFHPNWTASVEGEKTPVFRANHTFQALYIGRAGKSRVVLKYNDPWLWRLHLAIPL
                     GILAILGGLWLPGRWKTRSPATTLLETNG"
     gene            complement(16692..18062)
                     /locus_tag="P5079_02135"
     CDS             complement(16692..18062)
                     /locus_tag="P5079_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28818.1"
                     /translation="MDILFVNPSLEWKADLQKKISIRIESNIPNQESPHIGIAYLLAM
                     AKKIGISAHYIDMVMQGWDVEKLMDFIAEHRPALVGFTAFTIQIKPAGKIAEKIKERF
                     PKTVVCVGGPHAGAVPGETLEEFPGFDFAVCGEGEHLLRRLLDVLGDEKALEKLPGVV
                     TRGKKDYAWTPIEDLDPLPFPAWEEFDLTKYPGTYPHRTRQELPMIVGRGCPFRCTFC
                     CRALGDDNRRRSVRSVLAEIEHNVERFGCESVAFMDETFVLPGKWMDEFLSTYMKRGL
                     HKKVSWSCSTRVSQITPDFLRRMREAGCYYIFYGMESADDATLRLIKKGITVQQIRNA
                     VKWSQQAGIIPVGAFIIGLPGDTEEKIHRAIDLARELNLYSVTFPIAVPFPGTELRDM
                     ALKKEYGMRILTNDWDLYGKQSGGVMESDDLSWQKRNELQKIAYQTHPKKKIAQYVEW
                     LEKVKN"
     gene            complement(18104..19057)
                     /locus_tag="P5079_02140"
     CDS             complement(18104..19057)
                     /locus_tag="P5079_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY28819.1"
                     /translation="MPNKTTEAKILITGGAGYIGSVLTRVLLDNGYRVTVLDAFKFRQ
                     PSLLDCCAEPSFEVVRGDCRDERTLKDLLKNADVVIPLAALVGAPLCDRDPIAAETTN
                     QTAVEMLCKQASKDQRILIPTTNSGYGVGEKGKFCTEETPIRPISLYGRTKMAAERAV
                     LEHGNGISFRLATVFGASPRMRVDLLVNDFVYRAVKDRAVVLFEGHFKRNYVHIRDVA
                     GVFLHGLKNFEKMKGKPYNVGLDDANLSKMELCQTIKKILPKFVFIEAPVGEDPDKRD
                     YIVSNARILATGFRPKFSIEDGVRELVKCYKIVQDTSHANV"
     gene            complement(19038..20045)
                     /locus_tag="P5079_02145"
     CDS             complement(19038..20045)
                     /locus_tag="P5079_02145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRY28820.1"
                     /translation="MEKRTFKRVIITGISGSGGSYLAEHIVREHPGAEVHGLSRWHST
                     AGSGNLEAIRDRVVLHECDMTDFSSLMSAVSEVKPDAIFHLASHANVRASFQTPLAVL
                     ENNIMNTANLLEAVRLAKLDPYIQLCSTSEVYGQVDPKDVPIREDCSLRPSSPYAVSK
                     VAQDLLGWTYFRSYGMKVIRTRMFAYINPRRADLFATSFARQVARIEAGLQKELVHGN
                     LDSVRTLIDVRDAMEAYWVALEHCAPGEAYNIGGTTVIKVGEFLDLLLKRSRVPIKTR
                     QDPALLRPADVTLQIPDTTKFFNATQWKPKHSFEESVELLLGHWRKEIAKEATHAKQD
                     D"
     gene            complement(20055..20756)
                     /locus_tag="P5079_02150"
     CDS             complement(20055..20756)
                     /locus_tag="P5079_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY28821.1"
                     /translation="MKTTLFIPVLNEIEAMRQVLPRLPKGCCDQILVVDGQSRDGSAE
                     YARSLGCDVYVQKEKGLRAAYSEGIQHATGEVIVTFSPDGNSLPEKIPELIDKMREGY
                     DMVIVSRYLGGARSEDDDMMTGFGNWMFTSLINFFFSSNYTDALVMFRAYRKDIVKRL
                     GFDKPVFFMEWFEKKIRTLSGWEPQMSMRCAKARLRVGEIPGDEPPRIGGVRKITPWR
                     SGAVLSVQILYEFLT"
     gene            complement(20766..21752)
                     /locus_tag="P5079_02155"
     CDS             complement(20766..21752)
                     /locus_tag="P5079_02155"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY28822.1"
                     /translation="MTAMDYYKNRKVLVAGSTGLVGMALTRRLSESGARVRAVVHTTP
                     PPVKYPGVEYVTADLTGKEDCRRVVDGMEFVFLVAANTSGAATISATPLVHVTPNIIL
                     NALMLEAAYTAGVLKFMWLSSTTGYPDTGARPVKEEEMYNGDPYDVYFPVGWMKRYTE
                     VLCRMYSTKLKKTMCVQVARPTNIYGPFDDFRWETSHVLPALIRKVVERQNPLEIWGT
                     GDDVRDVIYVEDFAEACLSILEKSTTYDPVNVGLGKGYSVKEMVRTLCEVEGYKDAKI
                     VFNSNKPSMIPVRLIDATKAAAKYGFKAKTDLREGIEKTAKWYRDNLAIVKR"
     gene            complement(21749..22750)
                     /locus_tag="P5079_02160"
     CDS             complement(21749..22750)
                     /locus_tag="P5079_02160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY28823.1"
                     /translation="MGFYKGKNVLVTGGTGMIGRPLVELLLAEGARVRVASMDDPGRC
                     PAGAEFMKANLIHWEECQKVVKDMDFVFNVVGIKGSVGIGVKRSASFMVPHLLFNTHM
                     MEAARQAKVERYLYTSSIGVYPPAEIFYEDDAFKLPPDNSDRFAAYAKRVGELQAEAY
                     GIEFGWDKIAIVRPANVYGPGDNFDPKTAMVVPALMARVVGGENPLVVWGDGSAVRDL
                     IYSRDCAKGMMLAMEKGACLKAINLGSGKGYSIKQVVETLCSLFKPSPTVQWDTARAT
                     GGKIRLMDMRRAKELIGFVPETSLEQGLRETLDWYLANKDKADKRYNVFHQKNYMEK"
     gene            complement(22777..23952)
                     /locus_tag="P5079_02165"
     CDS             complement(22777..23952)
                     /locus_tag="P5079_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY28824.1"
                     /translation="MMNVKYHLAEDTIDNQDINSLIEWLKGGPRLTKSRLTVEFEKKW
                     GEWLGLPYSVFCNSGSSANLLMYYVLSISGRLKNKKVIVPSVGWVTSIAPAIQFGFEP
                     VMCEADKDTFGLDLDHLETLLKKHNPSTVFMVQVLGVPHKMKELMALRQKYGFFLLED
                     ACAAIGASIDGKKAGSFGDMGSFSFYFGHQMSTIEGGMVSTPDKQFRDLLLMMRSHGW
                     SKDLDREDHEALVKKYGIDDFHTPFVFYEPGFNVRNTDLGAFLGLRQVAKLDWIISRR
                     QENHALYKKHLGDKFTIQKALPGAVVCSISFGALAKDTEERRRIVKALENNGIETRIF
                     SAGNLGLHPFWYNRYGKASFPMADRIHHCGFFLPNNPSLRPQDVDAISGVVLDAAKG"
     gene            complement(23952..24995)
                     /locus_tag="P5079_02170"
     CDS             complement(23952..24995)
                     /locus_tag="P5079_02170"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRY28825.1"
                     /translation="MSAGGRIIKFGEALREAMFLAMEQDPAVFVMGIGVDDHKAVFGS
                     TRGLVQKFGKARVFDIPISENALTGVAVGAALGGLKPVFIHIRCDFLYLAMDQLFNIA
                     AKWRYMFGGQMNVPMVIRCVIGRSWGQGAQHSQNLTSFFMHVPGIKVAMPTTANEAQG
                     LLLAAIEDPNPVVLIEHRLLYDIPGHVPERLGPIPFGRAEIRRTGKDVTLVASSYMAV
                     EALKAAEFLESQGIDVEIVDPVSLVPLDRETIVNSVKKTGKLLVVDSGWSTCGVGAEI
                     AALAAEKAFASLTGPVRRLGQEQTTCPVSKPLEKIFYPDAAKIAREVFDMMGRAFPDV
                     EVGGLTTTFKGPF"
     gene            complement(24992..25954)
                     /locus_tag="P5079_02175"
     CDS             complement(24992..25954)
                     /locus_tag="P5079_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY28826.1"
                     /translation="MDKKLAIELYRTMLRIRRVEEKVSALYPGDKIQSPIHLSTGQEA
                     VATGVCLAMASADHIYGTYRGHALYIARGADLKKLFAELYAKDTGCARGKGGSMHLAS
                     PENGLMGCSAIVASTIPVAVGDALASKMSKAKRVAVAFFGDGAVDEGVFFESVNFALL
                     KNLPVLFLLENNGYAIHSKVADRHKQTQLWRLGEGLGLKGRRYDGNDVFSVYAETKKA
                     IDGLRRGGAPRLLEFMTHRWGEHVGAGSDFNESYRLKGEEKRARSNDPLPKARRALET
                     KWKISRDVFDRWETELQSEIDAAVDFAEKSPFPRAERLWEDVFL"
     gene            complement(25961..>26259)
                     /locus_tag="P5079_02180"
     CDS             complement(25961..>26259)
                     /locus_tag="P5079_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28827.1"
                     /translation="YWIFRPLPWPRVGLFTLTLAFVALSFAGMWLAVRAGIPRTDLLV
                     VGMLLYAAPYALCAAEIYERYRAVMDPLLIGFSAYALCLSWDRMKGFFSKQKTA"
BASE COUNT         5340 a         8374 c         7205 g         5340 t
ORIGIN      
        1 aagctcccga tcgcaccgtc tgcctcttct gttccgccgc atacatttca ttgcgccgcc
       61 ggtcttcgtc ggtaaactcc agggaatcga acaggccgca ggattccaaa agaccccgat
      121 aggacgtggc ctcttccgga agacggacgg ccgccacctc gggcagaaat ttattgacca
      181 tcgccacctc gaattcgctg tcgtccatga acacgagact gtcgagcccg atgttcaatt
      241 ctttcgcaat ctctctcagg ttggacacct tatcgttcca gttgattctc cttgaaacga
      301 agtcgtccca tcgcagcggc atgtcggcat gggcggcaaa aacctcttgc acgtcctgcg
      361 gattgttccg gctgcaaaga gccaacaaca ccccccggcg ggccagttcc cgcaccgcct
      421 cctggaactc gtgataggcc gaccccggaa aggtcttgcc gatgagcacc cccccgggcc
      481 cgtcctcccc cgccacgccg ccccacagtg tgttgtcgca atcgagcacc aggcattttc
      541 gaacccttcc ccggcaagcc cgcacgaatt tcatgtactc cgcggcaaac tcctccaagg
      601 catggggtgc gtatggagcc cggccgatat gccaataccg ggcatcaaaa aattctttct
      661 gtccgacccg cgcccgcacc gattccacgt ccaccacata cgcgtcccga aaccgtttca
      721 gcgtcttgat cagttccaga ttcagtgccc gcaccgtgtt ggccgcccca tccagggact
      781 gaaaatcgag gatccccagg gccgggtaga gcggcgactc gaaaccgtgc accaagatgg
      841 gagcggcgct gtgccggcgt atcgcctcca ccaccgacac gacgtattgc aaaacgcgag
      901 aggtctcttc ttgccgttgt tgcggagaca ggccgccgaa tccccggaac aacgccgggg
      961 ccagcgattc cagccgcaac gccaccacgc agacgtccgg aggagcggcg tataggccgc
     1021 tcttcccgtt cgaggcgtcc tgaagcgccg tgtcataccc gcccacggcg atctgcggcc
     1081 gcagtcctgc ctcaaaacac aggagcttca cgtaggggaa cagcatgtcc agcgtcacgt
     1141 tggacaggaa cgaccaccgg atcaccggtc gctcttcgtt ccgggactcg tattccttca
     1201 attgtttcag cacatcggcg taatggaggg atgtattcat ttccctcctc cggtgcccgg
     1261 cgattgttct tccacaagct cgacgatttc gttggcccgc gtgaaataga aggcgacccg
     1321 gcggccgccg aacaaaaccg ccggccgggc gctgcgcacc gacaccgccc ccaactgttc
     1381 caggtgcgcc gatgcggcgg ccaggtcctt cacccggtag caaacatggt tcaacccgcc
     1441 gccttttttc atggcgcggc tgatgggcga agtcggcgag gtcggccgaa tcagttcgat
     1501 ggcctgccct ttttcgtccc gcatcaacag cacttccgcc tcctgaagcg gatccgtcac
     1561 cggcgccccc tccgccgcat atccgaaatg ccgggcgtaa tagacggacg cctcctcaat
     1621 ggattccacc acgataccga tgtgatctat gatcatgttt tccccggccg gcaatagaaa
     1681 cggatctgtc ggcccaaccc caccgacgcc aattgacgat aaaaccgccg ccggacatcg
     1741 tcccggcccg tcttcgaaag gactctctcg aaattctccc gcatccggtg ggcttccctg
     1801 ccgcgttccg gatgagacac gtaatcgatg cccatcagca acagcaggtc tatgggaaaa
     1861 tcgctctcct tgcggacgat ctcgaacccc tcgcgctgca gtgcgccgac gacctggtcg
     1921 tgcgtccaat aggtaatgtg ttccggggcg atccaccaac agggaagccc catctgctcc
     1981 acggcggaca attggaacgg attaaaatcc aacccgtgat cgagaaggaa tatcccgtcc
     2041 ggccgcaaaa acgcgtgaca cttgcggagc aactcgtacg gctcaagcac atgttccaac
     2101 aggtccgcca cgtggacgac gtggaactta tcttccgccg atgggaccgg aacgttttct
     2161 atgaaatcgg gggagcattc cactccgtat ttatcccttg ccagccgggc caacggactg
     2221 ggctctatcc cccgaacctt ccatcctcgc cgctggagat acgccaggaa catcccgttc
     2281 ccgcacccga cgtccagaca tttccacggg gcggatccgc cggggagcag ttcttccagg
     2341 ttgacgcgtt tgtcctcgaa aatcctctcc cagaactccc gctcctcatt ttgcaactcg
     2401 tcccaaccgt ctttcacttc ctggaaataa cgtttttcgt agaacgtttt gatccatgct
     2461 ttcgtaggca agggctcctg ccggcggcac cgccattccg gatcgaaaac gacccggatg
     2521 cctttgtgtt cccagaacgg cgcttcttct attcttccca tcttttcccc aacccctatt
     2581 ttggcggata tcgcagcgcc caaccattcc cttctttttt ccaaatctcg ccgttgcggt
     2641 gctgatccac gatccgatgg aattcctcct cttcgatgcc cagataatcg agcatttccg
     2701 gaaaaaaatc cctcgggaac tccgcgtcgt attttcgagc cttctccagc ccctccccgc
     2761 gcgtcatctg tccgttctgt atcatgcggc aggcgtcgcg ggtggcccga ccgaacccga
     2821 atttgacgaa ctgcatgtag taataaatgt tgtcggactt gtcgtccagg ctgtcgaaat
     2881 ccgtaaaggt gccgtccgtg cgccccttgg gacaggtctg aaatggaatc ctgtccttaa
     2941 tgaagttgta gttgttgagc atgctccatc ggaagaagta cccgaaatag agggccttga
     3001 cgccgacccg tttcacctcc tcggcggacg gatacagata gggattaagg tccttcaccc
     3061 ccaccacgtc gtcgatccag ttgcgcgggt cgtctcccac ctggtgttcc aacacctcgg
     3121 tgaaatcgcg gaccttcttg gattcctccc gaaggacctt cccgccgtat tcgctctccc
     3181 cgtgctccgc gtagaacacc agcgggatgt tgtaatgaca ggccacctgc accggcacgg
     3241 tgttcactcc cgcgtcccag gccaccttct ggttgccccg ttcgcggaag aagcgcctcg
     3301 ccaaatggcg atggatcttc tgatcgggcc ggaagaaaag atggtcgaag ccgcaacgga
     3361 tgagcgcttc ccggttattc atccccaccg cgttgggcag ttgcggagaa aacgtgacca
     3421 gcagcggatt catgccgtat tcgaacttga gccggtaggc gatggaactg ctgtccttgc
     3481 cgccgctcca aggcacgatg caatcccagg ccccgtcttt ggaacgatac ggctccacca
     3541 gggagaggaa ctcgcggcgg cgcgcctccc agtcgatctt ctttttttca tcggcgtgac
     3601 gacaggcgtt acagacccct tcccggtcaa actcgatcct cggacgcgtg tccggcatga
     3661 tgcagcgtgt gcaatacctc accatgattt tctcctctgt cctctcgggg cccttacgcc
     3721 gtccgaacgg gaatgccggc ggtcgccagg tacttcttcg cgcttcgaat gctggtgtcc
     3781 gtgaaatggt agatgcacgc ggtgcagacc gcggcggctt tcccctgaac gaaaccgtcc
     3841 acgaaatgct gccagttacc ggcgccgccg caaaccaata cgggtatttt cacggcttcc
     3901 gaaacgagcc ggttcaactc gttgtcatag ccttccagag aaccgtcccg gtcgatggcg
     3961 gtgacgaaaa tctcccccgc ccccaactcc tccgctcttt tcgcccagcc ggcgggagat
     4021 tgtccggtgg ggcgagcgcc gaaatgggaa tagacctcgt agtccccccg ttccttcttc
     4081 ttggcgtcca tggagaccac cacgcactgg gcgccgtaga gggccgccgc ctcggagatg
     4141 aattccggcc gttccagggc ggcggtgttg atcaccacct tgtcggctcc caaattgaga
     4201 agccgccgaa aatcctcgat gttccggacc ccgcccccgg ccgacagagg cacaaaacac
     4261 cgctcggaaa aaagttcgac cactccataa aaattttccc ggtcccccgt ccccggtcgg
     4321 gtcacgtcca aaacgacgat ttcatcgacg gcccacgcgt cgacgaaatt caaggtatac
     4381 cggtaatccg gctgaaactc cttcgtccgg aacaacacgc cgtcgttaaa cgtcaagacc
     4441 gtgacaaggc gttttctaag cattgtcgag aaagttcttc aggagatcga gcccctgccg
     4501 ctggctcttc tccggatgga actgcgtggc gtatatgttg tccctttgga gcacggcggg
     4561 aaatttcatc ccgtagtcgc actccccata caccaaggac ggatcgtccg gttgcacata
     4621 atagctgtgc acgtaataga acagggcgtc cggcgctatc ccgcggaaca agatgctttc
     4681 gcgcttctgg accaaatcgt cccaccccac atgcggcacg cgcagcgcgg ggtccgccgg
     4741 gcgcagccgc acaacggccc ccgccagcca gcccaggccg gcgtgacggc cgaactcctc
     4801 gctctcccga gccaacaatt ggcatccgag acatatcccc aggatcggtt ttttccgtcg
     4861 gattaccaat tccgtgagag tctccaccag tccgcgctcc ctcaaatttt tcatgccgtc
     4921 accgaacgcg cccacccccg gcaggatgag tttgtcggcc ttttccagtt cctccgttcg
     4981 acaggtgatg cggagggcga atcccgcgcg tttcaccgcc cccgccacgg aacggaggtt
     5041 gcccagccca taatccacaa tggccactgt tccggtcccc atggcccgcc tcaggaaaac
     5101 tcctccagga aaagttttgt ggagaggaaa tagaaaagga atttgctctc gctgttcggc
     5161 agttccttct tatccaacaa gacctcgatt ttctcccggc gaacccaatc gaaaacggga
     5221 ctatctttca agagagcggg cttcacgcgg ggatcggccg tgtccaggaa ggaccgaatc
     5281 ggggcgttga accccacttt tcgcggattg tccaaaatcg gatcgggcac gatcccccgc
     5341 agagcctccc ggaggacggc cttggcgcgg ccgtttcgga caagatgccg gaccgggatc
     5401 cggccgcaat actcgaagag cgcccgatcc aaaaaggggg acctgttctc gacggaaaaa
     5461 tacatggcat tcaggtcgtc ttcatgcagg atgaccggga ccgcctcgta aaaaagttcg
     5521 ttgagcatcc tgtttcgaag aaggcctccc tgggtcagcg tttcttcccg gaacggttcg
     5581 gcccattttt ccgtcaagta gccggcgaaa tcgtcggcct ccaaataaat atgtttccgg
     5641 aaattcggat ctcggacgaa caggtccggg tcgccgagga aagggtttct cacccacggc
     5701 cgtacatttt tttcccaagc ctcgagcgca gccccgtgca gggcacggtc ctcgcggaca
     5761 tcgtaaagat aggccaggtg atggtcgtaa tatcccgaaa acaattcatc ggcccctgtt
     5821 ccgctcacgg aaatccggta acgctgatcg gcaacgctct tcatcaagag ccaatgcgcg
     5881 taataggaga tcgtgaatac cggagcgtcg tgctgccgga caagcgtcct catgttcgcc
     5941 aaaaaatcgc gcgtatcgac cgggatggac gtatggcgca gccccaggga tttgacggcg
     6001 caatcgacca tttcctgttc ttcgtaacgt cggtcggtgt tggcgatggt aaacccgtga
     6061 acatcgtatc ccaattcttt tttggcgatg ccgatcagcg cgttcgagtc gacccccccg
     6121 ctcatacaga aggccagagg cacatccgcc cgcaaacgga tctccaccgc ccggaaaagg
     6181 cgctcccgca cgccagacac ggcctgctcg aacgtcatcc cggcgtccgc tccgatggac
     6241 ggcttccaat agcgtttctg ttgaacccga ccggagccct ccacccgcca aacgtgggcg
     6301 gcgggaagtt cctccaagcc cttgaaaaag gtctgtcctt ccttgtaaag tgatttgtac
     6361 ccgttcacca ggaaccgttt catctgttcg tgatcaattt ccagccgccg gcctagcagc
     6421 gcggcaataa acttggtctc cgaaccgaaa taaatccctg acgcatcccg atggacatag
     6481 agcggcttct ccccgaagcg gtcacggcaa aggacgagcg cgtcttcctc ctcgtcgtat
     6541 acggcaaagg cccacatgcc ctcgtaccgg tccaggactt cccatccaaa aacttttatg
     6601 ccccgcagga acacctccgt gtccgaagtg gtggaaaaaa cctctccctc ggcctcgagt
     6661 tcccgccgga tctccgtata gttgtacagt tcgccgttat agaccaacca tttcttcccc
     6721 acctgaaagg gctggttgga ccgcgcttcc aaatcgatga tattgagccg gctgtgcagg
     6781 aaatggatgc ggcggccgga acgcatggtc caatccttcc gagcggcgtg gtcgggcccg
     6841 cgccggcgca tcaacgccag acatccgtcc aaacgacggt cgtccagaac agcggtcccg
     6901 atatagccgg cgattccaca catgaaataa gctccgtcta ccccacgtcg atcggcgata
     6961 gaaattcgcc gggccgcacg gttcgtttca gtttcttccc caacagccgc ttttcctgtc
     7021 ccggcggaag gccttctccc ggacggatcc acatgaggtc ttcccgcgtg agccggtgtc
     7081 cggccgggag gtcccgggac gcggcgatgg accgccggac ggatggggcc atacgacgtt
     7141 cttccggccg ggcctttttc tccagctctc ccaacataag tgaaaccgtt cggacccttc
     7201 ggaccaactc cgccagttcc cccggatcgg cggatatttt atggtcgcgg aaatcggaaa
     7261 aatttttgtc caacgtgaaa tgcttttcaa cgatgcgcgc ccccaacgcc acggccgcaa
     7321 caaccgcttc aagacccgtc gtgtggtcgg aataacccac ggcgcagtcc atcttctccg
     7381 ttagaaaacg gatggccctc aatcccgcct ggtccggcgg cgtcgggtaa agactgacgc
     7441 aatgcagtac cgccgtctcc ccggaaacgc cccctttttt tcggcaacgc cccatgaact
     7501 tcaccgtttc gaggatctcc ccgtaatcgg tcagaccggc cgacacgatc accggtttcc
     7561 cggtcaaaac caccttttcg atgagaggat agaacgtgtt gtcccccgaa gccaccttaa
     7621 acgcatccac gatcggcgcg aggaactccg cgctgtccat atcgagcggg gtggagatga
     7681 aaagaaggtt caaggaatgg gccaaagcgg cgagcttttc gaattgaggc tgggtcagct
     7741 caaaagactt cagccgctga aagcgagcgg cgtccctggc gctgacgaat ttttcggtct
     7801 tgtacgtctg gaatttcacc gcgtcggcgc cgcattcggc ggcgcgacgg accagttgtt
     7861 cggccacctc gaaacggccc tcatggttgt tgccgatctc ggccaccacc aaaacctttt
     7921 ccgtcgtatc gaaacgacct atcttcattt catcctcttg ctgtgggcgt gcatcacata
     7981 cgccgtgtct ttgacgcgaa atccacagtt ctcgtagaac ctcagggcgg gcccgttggc
     8041 cgcctgcgtg cccacacgca gccgatggca ccggcccgcc ccgccccgga ccaacgcccc
     8101 gaccaaagac cgaccgaccc cgtgtcccct catccgcgag gacaccgcca acagatcgat
     8161 gatgcgcaac gagagccgcc cctctctctt ggccaacacg gcgagaaaac cgaccgcctt
     8221 tccttttttc tcagccacca gcagttcatc gcctcttttc ccgtcgatgt aactttgaat
     8281 ccattctctt tttatccggt cggccacgcg gcgggggatt tccggatcca gatgaaagcg
     8341 tgaatattca aaactcgtcc cggcaatgtc caaaagccga tccgcgtcct tggccacgac
     8401 cggccgcacg cggcagcggc ccccaaaaaa ctcagggagg cgggccgcgg ggcggcgttc
     8461 catgaggacg ttggtctcca ccacatagaa ccccgcctcg ctgaaacgcc gcaaggacgc
     8521 gatatcccga gtggacgcgc gcgtgtaaaa aaatgccggg ccgcgtttgg cgacaacgcg
     8581 cattttcttg acgaggtcgc aatgagacct cgttccgttc gctgcgccgg agacgcggaa
     8641 agcggccttc tgtaaaatgc cggaaagcca cgcgtcttgt tcgagttcca tccccatctc
     8701 ccgctccttc aacctttgtg gatgtccttg taccacgcga tgtatttcga aaaacccacc
     8761 tcaatgggat tttggggggc gtatcccaaa agtttctgag cctttttgat gctgagcgtc
     8821 ccgcggaacg gcattaattt atcccgcgct acattctcta tctttgagcc ggggaaatgc
     8881 tgcgtgacga tgccggccag ctcattgatg gaccgggact gtccgtaggt cacgttgaat
     8941 gtctgattcc gcgagttcgg gttggtcaga atcacacgga ttccttccac caggtcgtcg
     9001 atgtgggtaa aatccaggcg ttccttgccg tccccctcca tccggagagg ttttccctgg
     9061 agggcgtttt cgatgaagat ctgccccacg cggcggctca cacagcgcgg tccgtagagg
     9121 gccgacggac ggacgatggt ataggggata tcgaaaacct gctggtaggc aatgacgatc
     9181 ttttccccgg ccactttcag ggccccgtaa atcccgatgg ggttcagcgg ctggtcctcg
     9241 tccacctccg gggtcagaaa gttcccatac gccatgctgg aggagaaata gacgaaatgc
     9301 tcgacgatgc cccggctggc atcgagggcg ttctcgagcg tgcgcaaact gtggtcaaac
     9361 gtgctgtagg gatccttgtt ggagcggccg gcatgggcca ccgccgccag atggatgact
     9421 atctgcggac ggatctggct catgatgcgg cacagggcgt ggtagtctct cacgtcctga
     9481 acatagagcg ggatacgggc ctttcgcaac aggtccagcc gctcttggat gaacctcatg
     9541 tagaggtctt tgtgctccga atcgttatcc tgcgatgaga accaaaggag attgttgacc
     9601 tgaaggccgt cgacgatgtg gacctccgcc ccccattggg agaaggtcag ggccatgtta
     9661 tggccgatga agcccgcccc tccgattaag aggatctttt tccctttgag ttcgtccgtc
     9721 atggcaaaac ctccttgatg acgcgtttga aatgggtgac gatgtaatcc aggtcgtccg
     9781 ccgtcagatg gggccccacc ggcagggcga tggactgatc gctgatgcgc gcggcttggc
     9841 gataatcctc cggacgataa ccgtattttt cccggtagta ggtcatccgc ggcaccggct
     9901 gggggtaata gacgctcgtc ccgatccccg cctcgttgag tttgaacacc gccttggcgc
     9961 gatgcgctcc caacggcccc tcgaggacga cgctcagaca ataacagctg ctttcctcgc
    10021 cggtggctcc gccgtccaaa acggatatgt taccagaccc tttcaaagcg tccttcagaa
    10081 acgcgaagtt ggcccggcgc cggcgaagga tctccggcaa ccgcgtgagc tgaacgcgcc
    10141 ccagggcggc gttgatgtcg ctcatccggt agttcagccc caaacttaaa acgtcgtaca
    10201 tccccgggat cgtcctctcg gaaaaactcc ggtccacccc gaaggcgcgc gccttggaaa
    10261 ccttgccggc gacggatttg tgccgagtga cgaacatccc cccctctccg gtggtgatgt
    10321 gtttgacggg ataaaaagaa aagcacccgg agtcgccgaa aaggccggca tgcttcccct
    10381 tcaatcgcgt tcccagggcc agcgcgcagt cttccaccac gtgaagtccg tgacgttccg
    10441 ctaacgagac gatttcctcc atcggacagg ggacgccgag gaagtgcacg attccgatgg
    10501 ccttggtccg cggcgttatc aaactttcca tcttggacac atccacgttc ccggacctcg
    10561 gttcgcaatc cacaaaaacc ggacgagccc ccatgatttc caccgcatgc acggtcgccg
    10621 tgtgggtcaa cgccgggacg aggacctcgt cgccgggacc cagccccatc tgataatagg
    10681 agaggtggag ggccgccatc cccgagctga cgctcaccac gtgggcgcct tcgccgagga
    10741 cggcagcaaa ttcggcctca aacgcctttc cctgcgggcc gtgcgccagc acatctcccc
    10801 gcaagacctc ctccacggca cggcgttctt catcgccgat ccagggcctt ccaaaaggaa
    10861 tcgtccgttg cgcggtcata cgtacctcac tttttcccaa gttttcgatt taagcgactc
    10921 atcgaacgcc gcggccacgc tgattccgtc gaaaaacgac tgcgctggga accttcggcc
    10981 cccccccttt aagaccgcgg aaacgaactc cggaatcaaa tcgcccttgc ttttcggatg
    11041 aggggaaagc gcgatccgga gcggcctttc ttccggagaa cggctggaga aaagccgtgg
    11101 gccggcgtcg tcgtagatga aggttttttt cgtgccgaag atccgcagga catgctggtg
    11161 cggatgaacg cacccgaagt tggcggtgat gcgccccacc agccccgaag cggtccgaaa
    11221 cgtggccgcg acataatcgt tgtagcggaa aggcgtgcct tcggtgcaaa tgctattacc
    11281 cgtcgacaaa actttctccg gccggtcgcc cgtcaaccaa aggaacaaat cgatcagatg
    11341 gatcccgccg cccagcatca cggagtatcc tttcacgcgc cggcgccatc ccgcggtgat
    11401 cttctccagg cggccgtaga ggtattcacc gtcgaccgcg tagatccggc cgaattcgcc
    11461 ggcggcgatg gcctttttca gccagcgata gaggggggcc gcccgaagga ccagattcga
    11521 gccgagttcc agacgtcctc gagactttgt ccactcccgt ttgacggccc gcaactccgc
    11581 ccggctgcgg cagagcggtt tttcgacgaa cacatgcttc cctgcccgca gggcttttaa
    11641 aacctgttca aaatggtcat cgtcgaagga agcgatcgaa acaaggtcga cgtccttgtc
    11701 gttcaacagc tcgtccgccc gcgacgacac ccggaccttc ggatatttct tttcggccca
    11761 ggcgcgtttc gccttcgccc ggtcgcacaa cgcgaccacg gaacaggcgc gaagtttccg
    11821 gtaagcggcc aggtgccgtt cccccacgcc cagcccgatg attcccacgc gcagcgtttt
    11881 tttcatgtca tgcccgtcct tcgaaagcag cggcggcccg ctgcaattcc agcaactctc
    11941 tccaaccgta ttcccaatgg ggcttcttca tcatggtcat cattctttcc gccaggagca
    12001 tgtcctgcgg cgtgtccacc gtcaatcgcg ccgcgccgaa atcgccgccg gactccagat
    12061 tgaaaatccg gaaagctccc aaggacctat aaaaataggg ggtgacgtgt tcccggtcct
    12121 cttccgtgcg catctcggga taggcccgaa aaaacgcctc tctcccgaca acctccacgg
    12181 actggccccg aggaaatgtg cgcttcaaca catcggtcac aatgtcgtgg tcccccttac
    12241 ggtagacccg gacacaatcg tcgatcaggg ccggatcgat gaacggactg tcgccgctga
    12301 tacggacaaa agcgtccagt ccgtattttt caaaaacccc cttaaacctt cccgccacat
    12361 cggtcaacga tccccggaaa aaaccgatgt gctccttttg acagaaatcc gccagcgcat
    12421 catcggtccg atcgtcggag gtggccacca gggcctcgtc caccgtggtg gcgcggcgga
    12481 cacgctccca aactcgggcc aacaggggtc ctccgcccaa cactttcagc accttgcccg
    12541 gcaaccgctg cgacgtcatc cgcgcctgga caacgaccgc tatcttcatg gggccgtcct
    12601 cctcttacgt caaaaaccga ccgatcttgg gcagaagggc catggctttg cggccgcggg
    12661 ccagcaacac tcggatctcc tgaagctgtt ttttcgtctc cgcgttcatc tcggaaccgg
    12721 gcgattcttt ggtgcgggtt aaaaaatcat ccagacgttt ttccgcccag gagatgcgtc
    12781 gtcccaaccc ctcctccagc cgccgcaggg cgacctcttt cagatttcgg ttggcctcgt
    12841 aatggtcccg acggctgccg gggatgggaa cggaacggac ggccccccac tcctccagga
    12901 cccgcaggtt gatgctggcg ttccccttgc tcatgagcaa acgctttgag atctcatcga
    12961 gggagagcgg ctccgggttc agatagagca acccgtagat ctgtccgatg gacttgttga
    13021 aactaaagct ctccgccagt tggccggcga agcgcacgaa ctcgtcttgg ggagaagtgg
    13081 gggtcatagg ccatcctttt tgtacgttta aaacgttcta aatattattt acatccaaaa
    13141 tgccgcgttg tcaagagttt tttacaacag aggcctgtga tgcttttgat gcgaaaaaac
    13201 gactcaaata tggtgggact gaaaaatacg actgggcggc gggggcggag aggagaaggg
    13261 cggggaggaa gccctatcgg ccggcgggaa actgccagcg gagagaatct tccggtttgt
    13321 aatacacgat ctgactgcca aaattctcga attcgaaggg gatgtgcaaa aatttcttca
    13381 gggattcccg gatctgcccg tggcgttccg gcggagcaaa gagcagaacg aacccgccgc
    13441 cgccggcgcc caaaagtttc cctcccaccg ccccggcttt cagcgccgca ccgtacagat
    13501 cgtccaccag cggggtggtt atcccgtcgg ccagcgtctt ctttatgttc cagctctcat
    13561 gcagcagctt cccgaacgcc gtcaggtcgg cggaactgtt caggatcttg agcgcctcct
    13621 tgaccatctg ctggatggct ctcagttcct cttttttctt gggcgtgttt tggatgatgt
    13681 ccccggccaa tttaactccc actcgcgaca gcccggtgaa cacaagcatc aaatgcgact
    13741 gaagctggtt catgcgttcc ttcgatacga tcaggggcgt caccagaatc cggtcatcgg
    13801 gaccgaactc catgtggttc agccctccca cggcggccgc caactggtcc tggcacccca
    13861 cgttctcccg gatgacattt tgttcaatgt gaatggcctc atggcccagt tcaagattgc
    13921 cgaccacctt gccggtgagc gcgtaaagac tcttcaacaa gcccacggtg aaggtagagc
    13981 tggatcccag accggtccgt gccggcagat cgccgtcgta gtggatttcc aggcccggct
    14041 ggattttcag atagttcaag catccccgca ccgaggggtg ctggatctct ccgatctcct
    14101 tgacatgctc gttcaatgtg taggagatgc ggtatttata ttcgaaaaac ggcggaaggt
    14161 agcggcaggt gatataggcg tatttgtcga tggacgtgga aaggacggcg ccgccgtttt
    14221 cccgatacca tgtcggataa tccgttccgc cgccgaaaaa agaaattcgg aacggggtct
    14281 tggtgatgat catgcgcgtt ttgtcctctt cccggagaga ttgtcgagtt tatatcaatt
    14341 ttctatccat ttgtctcaag gagggtcgtc gccgggctcc ttgttttcca gcgccccgga
    14401 agccaaagcc cccccaagat cgccaaaatg cccagtggga ttgccaggtg caaccgccac
    14461 aaccaggggt cgttgtattt caaaacaacc ctgctttttc cggcccttcc tatatagagc
    14521 gcctggaaag tgtggtttgc ccggaaaacc ggcgtttttt ccccttccac ggacgccgtc
    14581 caattcggat gaaaattatg ggagaccacc aggaccgcgg ggccttcggt ttcggcctca
    14641 aaaaccaact catccggccc ggcccgctca agtttcaatt tcttcttccc aacgaaagcg
    14701 cccgccgccc acgcggccga cggcgcggaa gcatcctcct gggaaaagaa aaccgtgtcc
    14761 ttcaacttct cgggagaaga cgccatctgc gacaatacct ctttcccggt cggcaatata
    14821 accgccgcct ccgccaaata cgcgcgttcc tggggatctt tcagcgcata gacgtaaagc
    14881 gggaccggtc gccacagttc gaccagcggc cccggcaaaa accggaacca ttttttttcg
    14941 tccttgatga ccgtcacctc ttttgaaacc gtctccattt caggcaatgc cccgggagaa
    15001 acgaggtgcg tcaaatgcgc cgccttcaac atcggcaaat tgattttttg caatagaccg
    15061 tcggtcggcc cccgcctcag atcgccgttc gccagataca ggttatacca gtattcataa
    15121 aactcttttg cctgttctgc cgtctgaaac tgaccctcga tcgccttgcc gatgaattgt
    15181 ttatagggcc cgtagaaaaa gggcccgcgc ccgtccaatg tctccagccc caccgacatc
    15241 accggagccg gcgggagctg ccaggaaccg atccggcccg ccgccgaacg ggccagcgcc
    15301 gatttcagga gctctgcgtt tccgaaatcg cgttcgtagg aatgatgttc ctgcgccacc
    15361 acccgatacg cccgcgcaaa gagcgcggct gtcacgaaca aagcgatggc cagcggccgg
    15421 ccgaggcccg tcccttcgcc ggcggaacga cggacaaaaa agaaaagtcc gccgaacatc
    15481 aacaggttgg ccgggatcag gaacaaccgc tcatccggcg acaattcgaa ccatgaaacg
    15541 cccaacaccg cgaccaggac aaggagaatc ccccggccgc cgccgaggcc tcctctcgaa
    15601 tgcgccaggg ccacgaacgt cgtcaccgtc gcaatgaatc ccagcgtcca gaagtagtgt
    15661 cctagatcca tcttcggcaa gaacgtgccc gccaggaagt tcgaggcgct ggaataaaaa
    15721 aaggcggaaa cggccagaaa aaaagccccc agagacagcc aatgtctcac ccttgcggag
    15781 cggctccaca caagcgatac ggccaacgga agaacaacca gccccttgac gggaagcgat
    15841 accgcgaact cggcgagata cgaaaaaaaa ccgcgcacat tcggcgaata aatccgggag
    15901 gtcatcaaac tgtaatcata caaggcatac aattgcggcg cgaacaccag gatatagccc
    15961 ccccacaaca aggcccattg ccacaggagg ccttttcgtt tataaacatc gttcccggcc
    16021 acataccacg ccaagaccag atgcaacaga ctgaaaaatg ggatcgtcaa cgccgcgtag
    16081 gagaagaaga ccgttgccag cacccccagc gacacccccc aggaaatcct ccggccctta
    16141 aggatcgccc cggcggtcca gacgaagaaa agcgggaaag cgtagttgta gaccacatgc
    16201 acaatgctct cgttctgaat ctgtgtgccg acggcgaaga caaacccgcc gatggacgca
    16261 agcggcctgt ccagctcgag cagctcgttc aggaagcggt acatgccata ccccgccacg
    16321 gccatgagcg tgatggtcag cgcgccgtaa aggaaccaga gcgggaaaaa ctgcgacaaa
    16381 agacagagcg ggtgataggg cggggaggtg tgcgcatagg ccgggctacc ccccgcggcc
    16441 acggggtacc aggcaaaaaa gccgtgccga aacaacagtt ctcccgccgc cttgtaccgg
    16501 gcgaattcca cgtcgaaggt gtcgtgaaga cggatcagcc catagggccc gaggaactta
    16561 tccgcgtaaa aaaccgccgc catcagaaaa aaatacgcga cggccggtgt ccggcggagg
    16621 gaatcaaaaa aaatcttaaa agagggcggc atgggggctt cttgggggga tcggcgggcg
    16681 cacaaaaccg gtcagttctt gactttctcc aaccactcca cgtattgggc gatcttcttt
    16741 ttcggatgcg tctgataggc gatcttctgc agctcgttcc tcttctgcca ggagaggtcg
    16801 tcgctctcca tgacaccgcc ggactgtttt ccgtaaaggt cccagtcgtt cgtcaatatc
    16861 cgcatgccgt attccttttt cagcgccatg tcccgcaatt ccgtgcccgg gaaggggacg
    16921 gcgatgggga acgtgaccga gtacaggttc aattccctgg ccaaatcgat ggcccggtgt
    16981 attttctcct ccgtatcgcc gggaaggccg atgatgaacg cccccacggg gatgatgccg
    17041 gcctgttggg accacttgac ggcgttccgg atctgctgga ccgtgatccc ctttttgatg
    17101 aggcggagcg tggcgtcgtc cgcgctctcc atcccgtaga aaatgtaata acatccggct
    17161 tcccgcatgc gcctcaaaaa atccggcgtg atctggctca cgcgggtgga acaactccag
    17221 ctgacttttt tatggaggcc ccgcttcatg taggttgaca ggaactcatc catccatttc
    17281 cccgggagga cgaacgtctc gtccatgaac gcgacggact cgcagccgaa cctctccacg
    17341 ttatgctcaa tctccgccag gacggaccgg acgctcctcc gacggttatc gtcgcccaag
    17401 gcacggcagc aaaacgtgca ccggaacggg caaccgcggc caacgatcat cgggagttcc
    17461 tgccgggtgc gatggggata ggtccccgga tatttggtca aatcaaactc ctcccaggcg
    17521 ggaaacggga gcgggtccaa atcttcgatg ggcgtccacg cgtaatcttt tttccctcgg
    17581 gtgaccacgc cgggcaattt ctccagcgcc ttttcgtccc ccaggacatc caggaggcgg
    17641 cgcaacagat gctcgccttc tccgcaaacg gcgaaatcga agccggggaa ttcttccagc
    17701 gtttccccgg ggacggcgcc ggcgtgcggg ccgcccacgc agacgaccgt tttggggaaa
    17761 cgctctttta tcttttcggc gatcttcccg gccggcttga tctggatggt gaacgccgtg
    17821 aatccgacca gcgccggacg gtgttccgcg atgaaatcca tcagcttctc cacatcccag
    17881 ccctgcatga ccatgtcgat gtaatgggcg gatatcccga tttttttcgc catcgccagt
    17941 aggtaggcga tcccgatgtg gggcgattcc tggttcggga tgttgctttc tattcgaatg
    18001 gaaatcttct tctgcaggtc cgccttccat tcgagactgg gattgacgaa gagtatgtcc
    18061 atcttacccg ccgatcttcc gaaattccgc cacggtttcc ctctcagacg ttggcgtgcg
    18121 atgtatcctg cacgatcttg tagcacttca ccagctcgcg gacgccgtcc tcgatggaaa
    18181 atttcggcct gaaccccgtg gccaggatgc gggcgttgga aacgatgtag tcccgcttgt
    18241 ccgggtcctc ccccacaggc gcctcgatga agacgaattt cgggaggatc ttctttatgg
    18301 tctggcagag ttccattttg gagaggttgg cgtcgtccaa gccgacgttg tacggctttc
    18361 ccttcatctt ttcaaagttc ttgaggccat ggaggaaaac accggccacg tcccggatgt
    18421 ggacgtaatt ccgcttgaag tgcccttcaa agagcaccac ggcgcggtct ttgaccgccc
    18481 ggtacacgaa atcgttcacc aagagatcca cgcgcatgcg cggcgaggcg ccgaacacgg
    18541 tggccagacg gaaactgatg ccgttgccgt gttccaggac ggcccgttcg gccgccatct
    18601 tggtccggcc atagagagag atgggccgga tgggggtttc ctccgtgcag aacttcccct
    18661 tttcgcccac gccgtacccg ctgttcgtgg tggggatgag gatgcgttgg tccttggagg
    18721 cctgtttgca gagcatctcc acggccgtct ggttggtcgt ctcggcggcg atgggatccc
    18781 ggtcgcagag cggcgctccg accaaggcgg cgagggggat gacgacgtcc gcgtttttga
    18841 gaaggtcctt caacgtccgt tcgtcgcggc agtccccgcg gaccacctca aacgacggct
    18901 cggcgcagca atccaagagg ctgggctgcc ggaacttgaa cgcatccaac accgtcacgc
    18961 gatagccgtt gtccagcaac acgcgcgtca ggacggagcc gatatacccg gccccgccag
    19021 taatcaaaat tttcgcttca gtcgtcttgt ttggcatggg tcgcttcctt tgcgatctct
    19081 tttctccaat ggcccaggag gagttcaacg ctttcctcga aagaatgttt cggtttccat
    19141 tgggtggcgt tgaaaaattt cgtggtgtcc gggatctgca gggtcacatc ggccggacgg
    19201 agcagcgcgg ggtcctggcg cgtcttgatg ggaacgcggc tgcgtttgag gagcaggtcc
    19261 aggaattccc ccaccttaat gacggtcgtt ccgccgatgt tgtacgcctc tcccggcgcg
    19321 caatgctcca gcgccaccca gtaggcctcc atggcgtcgc gcacatcgat gagggtgcgg
    19381 accgaatcca agttcccgtg gacgagctcc ttttgcaacc ccgcctcgat gcgcgccacc
    19441 tgccgagcga acgaggtggc gaaaaggtcc gcccggcggg gattgatgta cgcgaacatg
    19501 cgcgtgcgga tgactttcat gccgtaactg cggaaatacg tccaccccaa cagatcctgg
    19561 gccactttgg acaccgcgta gggactggag ggccgcaggg agcagtcctc tcggatgggc
    19621 acgtccttgg gatccacctg cccgtatacc tccgacgtgc tgcagagctg gatgtacggg
    19681 tcgagtttgg cgagccggac ggcctccagc aggttggcgg tgttcatgat gttgttctcc
    19741 agcacggcca gcggcgtttg aaaggaagcc cgcacgttgg catgcgacgc caaatggaag
    19801 atcgcgtccg gtttcacctc ggacaccgcc gacatcagcg aactgaaatc cgtcatgtcg
    19861 cattcgtgga ggacgaccct gtcccggatg gcctccaggt ttccggaccc cgcggtgctg
    19921 tgccaacggg aaagcccgtg gacctccgcg cccggatgtt cccggacgat gtgttccgcc
    19981 agataacttc cgccggaacc gctgatgccg gtgatgataa cccgtttgaa cgttcttttc
    20041 tccatgttcg tcccctatgt cagaaattcg tacagaatct gcacgctcaa caccgccccg
    20101 gagcgccacg gggtgatctt ccggacgccc ccgatacgcg gcggctcatc ccccgggatc
    20161 tcccccaccc gaagacgcgc tttggcgcag cgcatggaca tctgcggctc ccagccggaa
    20221 agggtccgta ttttcttctc gaaccactcc ataaaaaaca ccggcttgtc gaaccccagc
    20281 cttttcacga tgtctttccg gtaggcgcgg aacatcacca gcgcgtcggt gtaattcgaa
    20341 ctgaaaaaaa agttgatcag ggaggtgaac atccagttgc cgaacccggt catcatgtcg
    20401 tcgtcctcgc tgcgggcccc tcccagatag cgggacacga tgaccatgtc gtatccctct
    20461 ctcatcttat cgatcagttc ggggatcttc tccggcaacg agttgccatc gggactgaag
    20521 gtgacgatca cctcccccgt ggcgtgctgg attccctcgg aataggcggc ccggagcccc
    20581 ttctccttct gaacgtagac gtcgcaaccg agggaccttg cgtattccgc gctaccgtcg
    20641 cgcgattgcc cgtccaccac caggatctga tcgcaacatc ccttcgggag gcgcggcagg
    20701 acctgccgca tggcctcgat ttcgttcagg acggggatga agagggtggt tttcacgcgc
    20761 ggcgtttacc gtttcacgat ggccaggttg tcgcggtacc acttcgccgt cttctcgatg
    20821 ccctcgcgca aatcggtctt cgctttgaat ccgtatttgg cggccgcctt cgtcgcgtcg
    20881 atcaggcgca ccgggatcat gctgggcttg ttgctgttga agacgatctt ggcgtcctta
    20941 tacccctcca cctcgcagag ggtcctgacc atctctttca cgctgtagcc cttgcccaac
    21001 ccgacgttga ccgggtcgta ggttgtgctt ttttcaagaa tcgagaggca ggcctcggcg
    21061 aagtcctcta cgtagataac gtcgcgcacg tcgtcgccgg tgccccagat ttcgaggggg
    21121 ttttgccgct cgaccacctt gcggatgagc gccggcagaa cgtgggaagt ctcccagcgg
    21181 aaatcatcga acgggccgta gatgttggtg gggcgcgcca cctgcacgca catcgtcttc
    21241 ttaagtttgg tggagtacat ccggcagagc acttccgtgt atcgtttcat ccagccgacg
    21301 ggaaaataga catcgtaagg atccccgttg tacatctcct cctccttcac ggggcgggcg
    21361 ccggtgtccg gatagccggt ggtgctgctc aaccacatga atttcagaac cccggcggta
    21421 taggccgcct ccagcatcag ggcgttcagg ataatgttcg gggtcacgtg caccagcggc
    21481 gtggcggaaa tggtggcggc gccggaggta ttggcggcca cgaggaagac gaactccatg
    21541 ccgtccacca cgcgccggca gtcctctttc cccgtcaaat ccgccgtcac gtattccacg
    21601 ccgggatatt tcaccggcgg cggagtggtg tgcaccacgg cccgcacccg ggcgccgctc
    21661 tcgctgaggc gcctcgtcag cgccatgccc accagcccgg tggaaccggc gaccaaaact
    21721 tttcggttct tgtaataatc catcgcggtc atttctccat gtagtttttc tgatgaaaaa
    21781 cgttgtagcg cttgtcggcc ttgtccttgt tggccaaata ccagtccaag gtctcccgca
    21841 acccctgttc gagggacgtc tccggaacga aaccgatgag ctccttggcc cgccgcatgt
    21901 ccatcagccg tattttaccg ccggtggccc tggcggtgtc ccactggacc gtgggcgaag
    21961 gcttgaagag cgagcaaagc gtctccacca cctgcttgat gctgtatccc ttgccgctgc
    22021 ccaggttgat ggccttcagg caggccccct tctccatggc cagcatcatg cccttggcgc
    22081 agtcgcggga atagatgaga tcacgcaccg ccgaaccgtc gccccacacc accagaggat
    22141 tctctccacc caccacgcgc gccatcaggg ccggcaccac catggcggtc ttgggatcga
    22201 aattgtcgcc gggcccgtac acgttggcgg gccgcacgat ggcgatcttg tcccagccga
    22261 actcgatgcc gtaggcctcg gcctgcagtt cgcccacgcg tttggcataa gcggcgaacc
    22321 ggtcgctgtt gtccggcggc agcttgaagg cgtcgtcctc gtaaaaaatt tcggccgggg
    22381 gatagacgcc gatgctgctg gtataaaggt accgctccac cttggcctgc cgggccgctt
    22441 ccatcatgtg cgtgttgaac agcaggtgcg gcaccatgaa actcgccgac cgtttcacgc
    22501 cgatccccac ggaccctttg atgccgacca cgttgaaaac aaaatccatg tccttcacca
    22561 ctttttgaca ttcttcccag tggatcaggt tggccttcat gaactccgcc ccggccggac
    22621 accggcccgg atcgtccatc gaggccacgc gcacgcgcgc gccttccgcc aaaagcaatt
    22681 ccaccagcgg ccggccgatc atcccggtgc cgccggtgac caaaacattc ttgcctttat
    22741 agaaacccat ggtgtctcct tttttctcct acgatttcag cccttcgcag cgtccaggac
    22801 gacgccggaa atggcgtcga catcctgcgg ccgcagtgag gggttgttcg gcaggaaaaa
    22861 cccacagtgg tggatgcgat ccgccatggg gaaactcgcc ttgccgtaac ggttgtacca
    22921 aaacggatgc agtccgaggt tcccggcgga aaagatgcgg gtctcgatgc cgttgttttc
    22981 caaggctttc acgatccgcc gccgttcctc cgtgtccttg gccagggcgc cgaaagaaat
    23041 gctgcacacc accgcgccgg ggagggcctt ctggatggtg aacttgtccc ccaggtgctt
    23101 cttgtaaagg gcgtgattct cctgccggcg ggaaataatc cagtccagtt tcgccacctg
    23161 ccgcagcccc aagaacgccc cgaggtccgt gttgcggacg ttgaagcccg gctcatagaa
    23221 cacgaacggc gtgtggaaat cgtctatccc gtattttttc accagggcct cgtggtcttc
    23281 ccggtccaga tccttgctcc acccgtggct gcgcatcatg aggagaaggt cgcggaactg
    23341 tttgtccggc gtggagacca tcccgccctc gatggtggac atctgatggc cgaaatagaa
    23401 ggagaagctg cccatgtcgc cgaacgaccc ggccttttta ccgtcgatgg aagcgccgat
    23461 ggccgcgcag gcgtcttcca gaaggaaaaa cccgtatttc tgtctcagcg ccatcaattc
    23521 cttcattttg tgcggaacgc ccagcacctg caccatgaac accgtggacg ggttgtgttt
    23581 cttcagcaga gtttccaaat gatccaaatc caggccgaag gtgtccttgt cggcctcaca
    23641 catgacgggc tcgaacccga actggatggc cggggcgatg gaggtgaccc accccacgct
    23701 ggggacgatc acttttttat tcttcagccg gcccgagatc gacagcacgt aatacatcaa
    23761 caggttggcg gaggaaccgg aattacagaa caccgaatac gggagaccca gccactcacc
    23821 ccacttcttc tcgaattcca ccgtgaggcg gctcttggtg aggcgcggtc cccctttcaa
    23881 ccactcgatg aggctgttga tgtcctgatt gtctatggta tcctcggcaa gatggtattt
    23941 cacgttcatc atcagaatgg ccccttgaat gtggtggtga gaccgcccac ttccacgtcg
    24001 ggaaatgcgc ggcccatcat atcgaaaacc tcccgggcga tcttggcggc gtccggataa
    24061 aaaatctttt ccagcggctt gctcacggga caagtggtct gttcctgtcc gagacggcgc
    24121 accgggccgg tcagggaggc aaacgccttc tccgccgcca gcgccgcgat ctcggcgccg
    24181 accccgcagg tgctccagcc cgaatccaca accagaagtt ttccggtttt tttgacggag
    24241 ttcacgatgg tctcccggtc caacggcacc agggaaacgg ggtccacgat ctccacatcg
    24301 atcccctgcg attccagaaa ttccgccgcc ttcaaagcct ccaccgccat ataggagctg
    24361 gccacgaggg tgacgtcctt ccctgtccgc cggatctccg cccggccgaa cgggatggga
    24421 cccaaccgct ccggcacatg gccgggaatg tcgtaaagga gccggtgttc aatgagcacc
    24481 acggggttgg ggtcctctat ggccgccagg aggagccctt gcgcctcatt ggccgtggtg
    24541 ggcatggcca ctttgatgcc cggcacgtgc atgaaaaacg acgtcaggtt ctgcgaatgc
    24601 tgggcgccct gcccccaact gcggccgatg acgcaccgga tcaccatcgg cacgttcatc
    24661 tgtccgccga acatgtaccg ccatttggcc gcgatgttga aaagctggtc catggccaga
    24721 taaaggaaat cacagcgtat gtgaatgaac accggtttca ggccgcccag cgccgcaccc
    24781 accgccacgc cggtgagggc gttctcggaa atggggatat cgaacacccg cgccttgccg
    24841 aacttctgca ccagtccccg ggtgctgccg aacaccgcct tatgatcgtc caccccgatg
    24901 cccatcacga acaccgcggg gtcctgttcc atggcgagga acatggcctc gcgcagggcc
    24961 tcgccaaact tgatgatgcg cccgccggcg ctcatagaaa aacgtcctcc cacagccgtt
    25021 ccgctctcgg aaacggactt ttttcagcga agtccacagc ggcgtcgatc tccgactgaa
    25081 gttccgtttc ccagcgatca aaaacgtccc gggagatttt ccatttcgtt tccagcgcac
    25141 gccgggcctt gggcagggga tcgttggacc gggctcgctt ttcctcgcct ttcaggcggt
    25201 aggactcgtt gaaatccgat cccgccccca cgtgctcccc ccagcggtgc gtcatgaatt
    25261 ccagaagccg gggcgccccg ccgcggcgaa gcccgtcaat ggcttttttc gtttcggcat
    25321 acaccgagaa gacgtcgttg ccgtcgtagc ggcggccttt caagccgagc ccctcgccga
    25381 gccgccagag ctgggtctgc ttgtgccggt ccgccacttt ggagtggatg gcgtacccgt
    25441 tgttctccag aaggaaaagg acgggcaggt ttttcaggag ggcgaagttg acgctctcaa
    25501 agaacacccc ctcgtccacg gcgccatcgc caaaaaaagc cacggccacg cgcttggcct
    25561 tcgacatctt ggacgccaac gcgtctccca ccgccacggg aatggtgctg gccacgatgg
    25621 ccgaacagcc catcaacccg ttctccggcg acgccaaatg catggagccg cccttcccac
    25681 gggcgcatcc ggtgtccttg gcgtaaagtt cggcgaaaag ttttttcagg tccgcgccgc
    25741 gggcgatata gagcgcgtgc ccccggtagg tcccatagat gtggtccgcg gacgccatgg
    25801 ccaggcacac ccccgtggcc accgcctcct gccccgtgga caggtggatg gggctttgta
    25861 tcttgtcgcc gggatagagc gcggagactt tctcctccac gcgccggatc cgcagcatgg
    25921 tccgatagag ttctatggcg agttttttgt ccataccctt ttaggccgtt ttttgttttg
    25981 aaaaaaatcc tttcatcctg tcccacgaca gacacagcgc atacgcgctg aacccgataa
    26041 ggaggggatc catcaccgcc cgatatcgtt catagatctc cgccgcgcac aacgcgtagg
    26101 gcgcggcata gagcaacatc cccacgacca acaggtccgt ccgggggatc ccggcccgca
    26161 ccgccagcca catcccggca aacgacagcg ccacgaacgc cagggtcagc gtgaacaatc
    26221 ccacgcgcgg ccaggggagt ggtcggaaaa tccagtagg
//
LOCUS       DAOTFD010000017        25623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1253_length_25623_cov_5.030663, whole genome shotgun sequence.
ACCESSION   DAOTFD010000017 DAOTFD010000000
VERSION     DAOTFD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 25623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25623
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_25623_cov_5.030663"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5079_02185"
     CDS             <1..965
                     /locus_tag="P5079_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRY28828.1"
                     /translation="ESFRRLAMEQMQGRLSVYLKDLRRRLVDLLALLEANLDFAEDEV
                     PDLSKDDLRAGLDRLLQEGTALLHTASRGRLLRDGLRAVLVGSPNVGKSSLFNALLGH
                     DRAIVTEVPGTTRDTLEERFLADDVPVTVTDTAGLRTETDRVEGAGIARAKRALEQAD
                     VALFVLDASRPLSEEDRAVAALLDGKKIVLALNKWDRAGKETAHHLRAPLPLPPKTPC
                     LKTCALDGRGVNELRRELVRVMDDRSPETADVPTLINLRHAELLNDAVNHLKAARRSA
                     ESSLHEECLAIDMRLALGGLNQITGEGVHDEILDAIFSKFCVGK"
     gene            962..1879
                     /locus_tag="P5079_02190"
     CDS             962..1879
                     /locus_tag="P5079_02190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY28829.1"
                     /translation="MNAFSFRFASFFPARKPLLAFVLALFFAAPAAFAKGRKPPEVVL
                     SPIPAALPAGRTLVIDCRSNVPLHNTALYWGDRKAFFYPDGPLAWKTLVGVPSQEMPG
                     RKQAVFYGELEKGQVYQATVTFTVLPTTFPVSRVSLAMEKDKLITSGQMERDAAELKK
                     IYQPRAISKKLWDGFFVFPTTGVFSSLYGTRRAYGGRVTRTGHTGLDIANDLGTPVTA
                     PNRGRVVFSGWLDSMGNVVVIDHGWGVFSYYLHMREATAQKGQLLRTGDPVGRMGSEG
                     VSTGSHLHWSVVVDGERVDPREWVERPFP"
     gene            1908..2810
                     /locus_tag="P5079_02195"
     CDS             1908..2810
                     /locus_tag="P5079_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY28830.1"
                     /translation="MHTHAHTEHLQRPRSQLTLALGITAFTFLVELAGGWWANSVALL
                     SDAGHVFMDASALSFSLLALHLSGRPATDRRTFGLHRLEVFAAFLNGALVMVVAGWIV
                     VESIGRLRVPEPVKTGPMLGMALWGLAANLFVAWRLHSFAKNDLNMRGAFLHVLGDAL
                     ASVGVVAGGIALRLTGLWAIDPLVGIIVALIVLFNAVRLLKDSLDILLEGVPRHLVLD
                     EVVDTIRKVPGVASVDDTHIWNICSHICSLSAHITVHAEKMPEQQKVVEHIGRILHER
                     FHINHSTIQTSSTAWKHGPTHPTR"
     gene            2848..4893
                     /locus_tag="P5079_02200"
     CDS             2848..4893
                     /locus_tag="P5079_02200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28831.1"
                     /translation="MKIFVPALMALFIAVFAAYFHTVHTARYFQPTMDDLLSALMPWG
                     EFLTAAWGPHLGRLAAALLFFASLPGLGARLLKLFRPTGETDLFVQSGAALAGWGTLG
                     FLLAAGGVASVSTLRVVSILLILLGLTGLRQADLSFPPFGRAGLKTGAGAGLLFFAGL
                     YLLTTVVPETFYDALVYHLAVPQAYLQSGRMVDLSDIHLTRLPGLIQTLYLWALAWSD
                     DRLCKLLNWGIGLLLTGALGAWVGKKWSPAAGRWAALLFLSSPMIGVNLWSCTNDLLC
                     GFFFFLSFSSWNDAWGEREDADMRRLLVAGLFFGAALATKYTAASGVFFFALDLIVRR
                     KRTQRLLAPPGLAFAAGVILLLLPWFFRASAWTGNPFFPKATALLGGDIPENLALLSH
                     WESEARGEGGLLFRTLALLRESLTGVDAGRFGFLGPVLLMLLPFLPFVRYGRPSAQLL
                     GCALVSYIFFAVTTGRLRYFIPQIGLLFALAAAAMEDYIATAVLLAKERNVAGRIADR
                     LGDVLKFLVSAVLFLNALWLVLIFQKFNQGWDVVWGRQSEAEYLRREHTGVYGHPSQG
                     AFDFLREADLSGRVFLVGDARTYRCPLPARASGAFNVPPFALWAKETPTPASFIERLR
                     REGYTHLLVNVPELRRITPSAYQAPEYAAFLGHVLDSLPSPLYRDPWSILFRVPAAG"
     gene            4898..6187
                     /locus_tag="P5079_02205"
     CDS             4898..6187
                     /locus_tag="P5079_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY28832.1"
                     /translation="MTPPEYLRKKSVWAWAMYDFANSAFATTVLAVVFNVYFVQRVVP
                     AEGVRLLGATVPGTSLWGYTVSLSMLIIFVCSPVLGALADHAGRKKSFLALFWLAGCA
                     ASAGLFFVRPGDVRPAILLFLVANIGFAGGNVFYNAFLSEIAEPGRMGRVSGFGWAVG
                     YLGGGLCLALNLWMIQSPQTFGLSSEDYLPVRATLLSVGVWWFVFGLPLMFVLRERPG
                     RATEHPAGLLRPAVLRLRETFQNLRRYKNLLTFLAAYLLYNDGIETIILMASVFGAQA
                     LGMAQNELILCFLMIQAVAFAGALFFGWLADRLSHKKSILCTLAVYGGVLLWAAAMRA
                     RWEFWILGAVVGVVLGGSQAASRSWMALLTPPEKSAEFFAFYGVVGKLTAVLGPLVFG
                     VVSQVWGLRPGVLSLLVFFLAGGALLTRVREKSESPA"
     gene            6171..7244
                     /locus_tag="P5079_02210"
     CDS             6171..7244
                     /locus_tag="P5079_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY28833.1"
                     /translation="MNLPPEEGTPALPGKNSRRRVRAKIILTILCGLGAGILACEFLS
                     RRFLPQFRLPRLAEEFRMGGDPLSPKRFSPSPDLGWEDRDASPPPDAFGFRSLHSATT
                     AKAPNEVRILVLGDSVGKQIFSCPADYEQAEGELSLAWGKPVKLWSFAVEGYNLDQYE
                     RILRYRAASVSADQVLILFCLNDVALQFVPVVFEHEGRLLMLQGIVKSPPRAVSPFLF
                     RHSHLYRAGWTFRNAYLYRKRFMPAQTFDSFLEERHRAFQNTVAWCRQREIDFSAVIF
                     PYFKPRDRYDREERASYASLRDWLSAAQAPVLDLHDKFSVDDLRAAHNQDPTDDVHPN
                     PSGYRKAREFILAFLKAHPLSNK"
     gene            7328..9181
                     /gene="mnmG"
                     /locus_tag="P5079_02215"
     CDS             7328..9181
                     /gene="mnmG"
                     /locus_tag="P5079_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRY28834.1"
                     /translation="MPERYDLLVVGAGHAAIEAALAGARSGFSTLLVTLDLSKIGQMS
                     CNPAIGGVGKGQVVREIDALGGEMAKATDRAGVQFKMLNRGKGAAVWSPRAQCDRRLY
                     REVMTDTLRRRPGLTLLEDEATAVGVRNGRVEDILTTKHGRIGGNVVILSTGTFLRGL
                     LHRGLQATPGGRLGERPSVGLSDSLRSLGFDVGRLKTGTPMRLDDRTIDHARCTRAPG
                     DEPPEPMSHFTRSLPQPQLPCWLTRTTAETHDIIRRNLDRSPLYTGKIQSIGPRYCPS
                     IEDKVVKFPHHGSHHIFLEPEGYHTQEIYPNGLSTSLPEDVQEQIVHSIPGLEKAAFL
                     RYGYAVEYDYCPPTQLKPTLETKAVEGLYFAGQINGTTGYEEAAGQGLIAGINAVLKL
                     RNEPPLILRRDEAYLGVMIDDLVTKGTDEPYRLMTSRAEYRLVLRWDNADLRLMDYGR
                     RVGLIPPDVHAHFVDYRRRVRDAVRAALPPDALEEPAAPPTDESLLPFPEAADRPPAS
                     ADRNFWDKRHIQRQVAIETTYWGYIKRQKQELAKFRKSESRRIPPEFDYDAIKSLLTE
                     SRQKLKKVRPESLGQAARIPGVTPADIAVLLVHLERRRRARRQGDTATRAS"
     gene            9193..9954
                     /gene="rsmG"
                     /locus_tag="P5079_02220"
     CDS             9193..9954
                     /gene="rsmG"
                     /locus_tag="P5079_02220"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRY28835.1"
                     /translation="MQKPFDFETHLTLEAAFQKWEPLFAETGVELGSAAGRLMSHFLK
                     ELKVWNRRVNLVSFHCDEELLLGHLLDSLAALQDPWLKNQPSLRFVDVGTGGGFPGLP
                     LVLARPEWRGLLLESVRKKTAFLETLVERLPLPTVAVLAERAEDAGRREEHREEYDAA
                     FCRAVGPFSTVLELTLPLVRVGGAALLHRGHEAPQETSAAGEALARLGGRLGGHTVYR
                     LPHLTQPRYIVRIEKTSATPEEYPRRNGRPAKRPL"
     gene            9982..11595
                     /locus_tag="P5079_02225"
     CDS             9982..11595
                     /locus_tag="P5079_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28836.1"
                     /translation="MKKPLLPALLLSAAVGGAALWHFTRLPLHEKGKKLLEQGDVTGA
                     VELFRRALEEKTWAPPVEREIRNELSRAYLLKGAVDNAEEEFQKTLERFPENAYAHIG
                     MGFLQLAKGFDNFALESFQKAKAADPDDLRASLGLASLYAYRGELPAARKEYDELVAR
                     APENRAIREGSGDVFFGEGLFNEAAAEYERALADLPENSTTRLAAARALLAAGRCDAA
                     EELTRRLVAGSPRASAARLLLADIYRDTGRFADAGPVYQALLSEDQRNVFALLRDAEW
                     QARRDDFETAKRRLDQAAQVMPKPGEAPVSFASLAEIEDFLEVRSYLRQAAVETGLAW
                     SKFYAYQGHYADAEREALRALQLFQKHLAALRWMAELQRIRGDGPKRLEWIRRARGFF
                     PRHALLLSDEAEALLALKKIAEARPPAEEARRSCPTLPRAAAVLAQARLAEGKISEAA
                     SAAAEAVALGPRDAQAHLAMALVLRSQKKYAEAETALRRSLELDPYNARAHDERAQVL
                     SLLKKPTDSVIAGREAARLEPTTYPLRKN"
     gene            11600..12277
                     /locus_tag="P5079_02230"
     CDS             11600..12277
                     /locus_tag="P5079_02230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY28837.1"
                     /translation="MIPIRDSVKSRSYPLVNVLLIAANLAMFVREITLSNADVLHDFI
                     SAWAVVPGRLVAFPWTDWPTIFSAMFLHGGWSHVLGNMMYLWIFGDNVEDRLGHFRYL
                     VFYLCAGTAATLAQVFLHPLSRVPMIGASGAIAGVLGAYFVLYPGAKVLTLIPLGFFV
                     RLVEIPAFFFLGFWFVLQAFQGWGSLLQSAAHGETGGVAWWAHAGGFFAGFVLVWFFK
                     RSGRRKR"
     gene            complement(12287..13594)
                     /locus_tag="P5079_02235"
     CDS             complement(12287..13594)
                     /locus_tag="P5079_02235"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY28838.1"
                     /translation="MRRRCLTWALAFFLPGPLLAAPLSRSDRKYLAGVYADTWRCLAH
                     FVSPTTGMPYDSHERLPSTSLTNAGFYMAACAAAQKTGLLSEEDALRRIEKVLDGLAR
                     VEKWRGFPVTWFNADTLQTTETQFSTVDHLGNLTAGLVLVRRAFPELRPKADALLAPM
                     NWGILYEPANHLYRGGYRLDTKDFDIRQPWGNWYYGYLGADTRLGSFFGVATGQVPLI
                     HWKSLDRSRETRSGQDYFVPGWQGGGLFMQCVSGLFFDERSTVLGKSAANFAYAQILH
                     AERIAAPAWGWSAAAAPTGEYLGWGALKDEVVTPHASVLAMSYYPRRATANLRALDRL
                     GARAPYVENGREYRFGFRDSFDWKTSRAAAGYLVLDQAMLFLALANVLHDGVVWDAVA
                     SDERVANGRKILEEYARLDGRVTEIYRRRDRKKISEALKIPLE"
     gene            complement(13618..25542)
                     /locus_tag="P5079_02240"
     CDS             complement(13618..25542)
                     /locus_tag="P5079_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY28839.1"
                     /translation="MVGKPVRDWRAAREAFKDILEWDRDTLQALARQAYEKKFGPLEE
                     GQFTLEKVRKFLKQTDKTALKERVQAMNLEAFQRFRNIAYAASGAAGAAAIVMLFIAP
                     LGGVPLVFLSALGFWNGIVLRWSHQAGRRVVELGRSAMPFEEKARWEYQLSVDYGTTV
                     ELLSTERMAALSGDENTVAFVHPEVPGTIYLNQGWAADRSARLQRFILPIVMAHEHVH
                     LEGVRLPTLPTRLPFAPWLNRWFLLPLNRKFFSLPLFHTHLFANLAMAARGILRRSRL
                     MFLDRARQDTVYLFDKHDVPGLTAKVVESALRDADILFLEIPHEWVPYYQAVSLGRLS
                     PEKAVELIGQESGYTSYFQSAVLGTLVKLYGSRVRIEGDPPLPPTILKLEQSAQEALN
                     QALLRLLEGDSAGAMRFFETHLTRMAEYEKERDALLHVRLRQINKLAPQSRIRVVRGF
                     VHSAHYGNIRQKLPAGKKGIVTDIPEGYEYLPYEILLHREMRGEAFADPAERRALMSR
                     AFFSALLSLDYDISQKKDIVSYSRRINALAAQVTPEQFEALAADLANHFQQDVVDNPI
                     VPATLKTSHVENWISNRLPGLVTSYLQDRGLLQAGEASVFHSLQPDTPMRQWKRRIKS
                     ALSRTSGAAGQGLYLSGAVALGSLVAPWLAAAAAMTRRDFLRGTSMALVGLWASNRFA
                     WAATPSPVQPKPVSPPSTEEDKALLARFEKLREVFRDELSRSVRVSSDYRLEKMGDIF
                     EELRFALENAAHLEDFVPGAFEEILQMTDFFTFYGQAEGAVSREPFEKLLPALLGQME
                     RSISRQDVVLPGVVGRTAAALVTAGSSAPDQIAGDSFALLSRNDRFVMAFLWGLKHDL
                     LVDYLDPRSSAKTVFQNRLREYIGPSWLVLLSELEAPLAGLAADTLSLEDIASRLEGI
                     QKRAEALDPWNNSIARLLYQRAMCRLALTEMLRDRQAGSPQNRAELREYMLNLTVFIY
                     KYLNAASTKEANAVVVHPLSTFLNGTAFFHYELARVAHEGRHQINMFKDLAGCLSVRE
                     DGVTVTIPISMKRRDELLKDVREARTDFHEDLSLLAAAYEGGRVLHTVLASENPALRD
                     AAVRDAFAGKKPEKVADRLTKRFDSSRKLDLDVRVRMRAVKVLELRLLDVLRGIQAAR
                     KVKTGKEAQALYFSVWEGEVEPFTEAKIMKKFRNEPVKQWEEFARLLRGGGLNLVYHP
                     DSYEMAARVYGMIYYWCRGDRNTIRDFVNAVSALPEDGGLSMAEAFEKYNRSPGAAAQ
                     KKKALEGPSVEGVMAWWPLLESWVRSLPSTETANKLWTELVTHLGIPDVRTGDLDGLA
                     AALKVSGRAFLLPYGLTVRLLEAAASAGGLEGPVVVPSTANPFQREFRQARKMLKEVY
                     GLRLSLLNVHLTRGPPGLVPTWTQDGHFLVPERTVERWVRQGKDPVKALVQTLLHEVM
                     ERHETLSAQAAGVVITREWQRVLHDRVSRHFNLSAASTARVVDLSELPDGPHFETKLK
                     EDGSDYSNLTEAREVLKTVQDLLARGLRLEDIVITSPYPGQLSLIRRVFGRSPVPEMR
                     YLSNFPSKDSHKVVIVSTVRSTAGALEGVRSNMPKDHLIRWRYHAALTEHPDKPDLVF
                     RRGRPLHVWQSRRGDYYIRTRVWNRPGDNLRTLRVDRFSLVSRLEILRGRSGWKKLTP
                     AELAELNAPSRVNPRHFAGLENVLKAGPQPIVIGSLPTLREDPAVDRLMSPLLDPYDP
                     PAEQEKLKPVLSAVEESVKKGEQPVVVFDLDDTLFDTRPRSLAILHGLARERMQRPGA
                     DLSGPRKVLAWRLEDMTGPWSVLLDRAAAVLGLTEEERKAAGARQYLPENFVHDRPLP
                     GAVRFVRELHARGARIVFLTGRFDAEERSLTMAQLRRFGVPNPEDDRRVSLLMRSPEF
                     RDNDPYGFAFKTASRDEIAKLGRVVAAFDDLSPNVETLRERFPEARVYHLNQRQTEET
                     VESPDVTLRGDYRLHRPLPFRAEPPVVDTGLHRWISPEELSEALTAFHELLPDSAITR
                     LAMSRGPPAAVLPEMDGHVRANSRGTVFFDDRFFEEARRKGLDVRAVLLIALLQKDHL
                     LRRRSEFRERNAGRAPTAENFEAWRREAMSLGLSLSSAAALLSLPFVQEKFERDRDLS
                     AGFLPIEKINGLLAELKAHHPTSVHHSGRVASYVRQAGRRLGYSPEQNEFLRQAGLLH
                     DIGKLGIPAALLDDSKPKLTDNEMDILRTHAIKGLVFLRERFPNSLVWLGTLHHQRVD
                     GKGYPRVPLKGRNRRLSGSEIPPIDRLLTIADAFDAMTSRRSYNVPMSRELAFKILGG
                     EPDQYSPCFLETLKQIFEKIPTSARGQFHLKQAARYAVLGSAGGLLLAALTAFGSPVI
                     AGFLGAASLWHGFAVLLNRRAGQKALRAGSFGTSQKRAAFKSVLEKLGVPTLANLSTE
                     ELRAQMETALTDIWGQQIRVRTTREMWETFGDERAVAEARREEGRPVLYINIGWLLEG
                     QSNRLRQFILPAILSHEETHLLGWSLPLASLPVNRLTRPLFAVNSFLKSRYFRTELSA
                     YAKTSAVTLIRGLMWWRWPLSKDQFYSPQPLFRRVMVFATLAAALNAATARLSLAAAP
                     DVPPPTPTTVTLPAVQAAAPEVETLVSETFGWARNFEQSPRKTYAEFRGEVTALLKEA
                     QARGQKPVLGIGETHGHLNEYRFAAELMGDVGREIPIRHYLEEQGGGEAHLFLNDDNP
                     RAGLIKELGAEVIFVTGNGPGVTTFPQPLKKADKGLAVVYAGKNHITAVIDWYPAVFN
                     AAGRYKVPPPTIHSQFDEQRPGVAVVMLQQSQIYEGMQEHFLRKLWEARSTLTEAQVA
                     QALERFEQEWAQYQATMGAEPRIVKSAGQENVYFVVLPNSIEMREPRAVKLFAAVWNN
                     PQRRQELLRSGVDKINVQDGMGRYMACGGASPLEASDLVPYGTFIGFTGRKDGKFSEI
                     RYYGPDGAAAPLPLVFRNDTGSVVTPQFAPAEREQFLKDLEKLKEEARRFENRHYEAK
                     EEDGRRTGAFFGDRAAPGVSVPAVPLAGMLSALGARRRRRDGPPLTPPGFLGTLEDYR
                     VYLESKDIRLTPVLENGRSLAVVPPELQPLAETGERVLRDLWDEGLLPGQGPLTVSVM
                     QFNMPGHEEAGLAKEINAVWTSDGHLIINENLYRKWLSHEWRGGGRMEESFRTQTRLQ
                     FGIHARALERAQAHRPVTREDVRLWTFDLAADFRQEKKWRSTLEPLTAWANHLRASPW
                     WSARPEKKGAALVRLAETYVTAGQPQAAKALALEVERLLTQAADPMEKARLRWGLMGL
                     FEKLGSRRKTAFYREAFLRLAASNPALMDFALAAGLKEVSGRPLAEGLAKAFRAVDLE
                     KTDAEVLASLFEALPRLSASSENAALLGELDDLRGTLPAGEISSRFGLALVLAYGKQG
                     NAARAGELFEDVSRTMSATLDKAPGLPPLEETYLAAAAQLGREQEARQVLAAVFARWM
                     ETGTRRGFESLGDLLRRLRLAAALKDSRLEALFGKAFAELDDPAPLPEKFEFLAGVIA
                     TFARVHPAPQRAASAQRRRERTLPLLPAVLPPVNGVSRAVRPSLRRIVGRRLSSVAAR
                     WGNGSEGLLSSELREFRYLPAAEMDRAASGFSEKMARALGERLEKTAASSASARGDIG
                     AASLEALGALHAMHQTLNPSAGTADLPQTEEVRALLRVLFENEFDLLPPIQRDFLKRF
                     GVRPAKTSGPDPADSRQALALARLLMAFGKGLPAGASDRETALEQKIKDLMKSRRPLT
                     GDEARRLEAAYQKGWVSSRWAQQGRHPWRIKALSRFLQAWEAEVPAQPVVKSYDLSGL
                     AETLRNREPLSPREMRLVRSLQEELVRRASSQETTAPLAVLSNGSVKEKDLLELLRTQ
                     IPSDLWDVLRDNVIVLSGGEVRSGFEGLMDPDRVLKTLEKRLGVPARAEIFTDDVERW
                     KKDWRSAAKALLIEVVSEMEFRIFRSSSDMEAEFRHIQLLQIQA"
BASE COUNT         4388 a         8747 c         7963 g         4525 t
ORIGIN      
        1 ccgagtcttt tcgccggctg gccatggagc agatgcaggg gcgtctctcc gtttacctga
       61 aagacctgcg ccggcggctg gtggaccttt tagcgctcct cgaggccaac ctggatttcg
      121 cggaagacga ggtgcccgat ctttccaagg acgacctccg cgcggggctg gaccggctcc
      181 ttcaggaagg gaccgcgctg ttgcacaccg cctcccgggg ccgtctcctg cgggacggcc
      241 tgcgggcggt gttggtcggc tcgcccaacg tcggcaaatc ctccctcttc aacgcccttt
      301 tgggccacga ccgcgccatc gtcacggagg ttcccggcac cacccgcgac acgctggagg
      361 aacgttttct ggcggacgac gtgcccgtga cggtcaccga caccgcggga ctccggacgg
      421 aaacggaccg ggtggagggc gccggcatcg cgcgggccaa acgcgccctg gaacaggcgg
      481 acgtggccct cttcgttctg gacgcctccc gtcccctctc cgaggaagac cgggccgtcg
      541 ccgccctgct ggacggaaaa aagatcgtcc tcgccctgaa caaatgggac cgggccggca
      601 aagaaacggc ccaccacctt cgcgccccgc tccctcttcc gccgaagacc ccgtgcctca
      661 aaacctgcgc gctggacgga cgcggcgtga acgaactgcg ccgggaactg gtccgcgtga
      721 tggacgaccg gtccccggag accgccgacg tccccaccct gatcaacctc cgccacgcgg
      781 aactgctgaa cgacgcggtg aaccacctga aggccgcccg ccggtccgcc gagtcctctc
      841 tccacgaaga atgcctcgcg atcgacatgc gcctggcgct gggcggcctc aaccagatca
      901 cgggcgaagg cgtccacgac gagatcctgg acgccatctt ctctaaattc tgcgtgggca
      961 aatgaacgcg ttctctttcc ggttcgcctc tttttttccg gcacggaaac cgctcctcgc
     1021 gttcgttctg gccctcttct tcgccgctcc ggcggcgttc gccaaggggc ggaaaccgcc
     1081 ggaggtcgtc ctctcgccca tccccgccgc gctgcccgcg ggacgcaccc tggtcatcga
     1141 ctgccgttcc aacgtccctc tccacaacac cgcgctgtat tggggcgacc ggaaggcctt
     1201 cttctatccg gacggccccc tcgcctggaa gaccctggtc ggcgtcccgt cgcaggaaat
     1261 gcccggccgg aaacaggccg tcttctacgg cgagctggag aaaggccagg tgtatcaggc
     1321 gaccgtcacc ttcaccgtgc tgccgaccac cttcccggtg agccgcgttt ccctggcgat
     1381 ggaaaaggac aagctcatca ccagcggcca gatggaacgc gacgcggccg aactcaaaaa
     1441 gatctaccag ccccgggcca tctcgaagaa attatgggac gggttcttcg tgttcccgac
     1501 caccggggtg ttctcctccc tctacggcac gcggcgggcc tacggcgggc gggtcacccg
     1561 aacgggccac acggggctcg acatcgccaa cgacctcggc acgccggtca ccgcgccgaa
     1621 ccgcggccgc gtggttttct ccggctggct ggacagcatg ggaaatgtcg ttgtgattga
     1681 ccacggctgg ggcgtttttt cctattatct ccatatgcgg gaagccaccg cccagaaggg
     1741 ccagctcctg agaacggggg accccgtcgg ccgcatgggg tccgaaggcg tctccaccgg
     1801 gtcgcacctg cattggtccg tcgtcgtcga cggcgagcgg gtggaccccc gcgaatgggt
     1861 ggaacggcct ttcccctgac gccgccggaa aagaggtcct aaaaggaatg cacacgcacg
     1921 cgcacaccga acacctccag cgcccgcgat cccagctcac cctggcgctg gggatcaccg
     1981 cgttcacctt tctcgtcgag ctggccggcg gctggtgggc caactccgtg gccctcctct
     2041 ccgacgccgg ccacgtgttc atggacgcct ccgccctttc cttttcgctg ctggccctgc
     2101 acctctcggg aaggccggcc accgaccgcc ggaccttcgg cctgcaccgg ctggaggtgt
     2161 tcgcggcttt cctgaacggc gcgctggtga tggtggtggc gggctggatc gtggtggaat
     2221 ccatcggccg actgcgggtc cccgaacccg tgaagacggg ccccatgctg ggaatggcgc
     2281 tgtgggggtt ggcggcgaac ctcttcgtcg cctggcggct gcactccttc gccaaaaacg
     2341 acctcaacat gaggggcgcg ttcctgcacg tgctgggcga cgcgctggcc agcgtcggcg
     2401 tggtcgcggg cggcatcgcc ctccgcctca ccggcctctg ggccatcgac ccgctggtgg
     2461 ggatcatcgt cgccctcatc gtccttttca acgcggtgcg tcttctgaaa gattccctgg
     2521 atatcctcct ggaaggcgtg ccccgccacc tcgtgctgga cgaggtcgtc gacaccataa
     2581 gaaaagtgcc gggcgtggcg agcgtggacg acacgcacat ctggaacatt tgctcgcaca
     2641 tctgttccct ctccgcccac atcaccgtcc acgcggaaaa aatgccggaa caacaaaagg
     2701 tcgtcgaaca catcggccgg attttacatg aacggttcca catcaaccat tcgaccatcc
     2761 aaacgtcctc cacggcctgg aaacacgggc cgacccaccc gacccgctga cgccctccgc
     2821 cttcccgtga aatccgcttc ccgctccgtg aagatattcg tcccggcact gatggcgctg
     2881 ttcatcgccg tcttcgccgc ctatttccac accgtgcaca cggcgcgcta tttccagccg
     2941 acgatggacg acctcctctc ggcgctgatg ccgtgggggg aattcctgac ggccgcctgg
     3001 gggccccacc tcggccggct ggccgccgcc ctcctcttct tcgcctccct gccgggactg
     3061 ggcgcgcgcc tcctgaaact cttccggccg accggggaaa cggacctgtt cgtccagtcg
     3121 ggggcggccc tggcgggctg gggcacgctg ggattcctgc tggccgcggg cggcgtcgcc
     3181 tcggtctcaa cgcttcgtgt cgtttccatc ctcctgatcc tcctggggtt gaccggttta
     3241 cgacaggccg acctttcctt tccgcccttc gggcgcgccg ggctgaagac cggagcgggc
     3301 gccggactcc tcttcttcgc cgggctctat cttctcacca ccgtggtgcc ggaaactttc
     3361 tacgacgcgc tggtctatca tctggccgtg ccccaggcct atctgcaatc gggccggatg
     3421 gtggacctgt cggacatcca cctcacgcgc cttcccggac tcatccagac cctctatctg
     3481 tgggccctgg cctggagcga tgaccggctg tgcaaactgt tgaattgggg gatcggcctt
     3541 ctcctcaccg gcgcgctcgg cgcctgggtc ggaaaaaaat ggtccccggc ggccggacgc
     3601 tgggcggcgc tgctgttcct ctcctcgccg atgatcgggg tgaacctgtg gtcctgcacc
     3661 aacgacctcc tctgcggatt ctttttcttt ctctccttct cctcctggaa cgacgcctgg
     3721 ggcgagcgcg aggacgccga catgcgccgc ctgctggtcg ccggcctgtt cttcggcgcc
     3781 gccctggcga cgaaatacac cgccgcctcc ggggttttct ttttcgccct ggacctgatc
     3841 gtccgccgga aacggaccca acgcctgctc gccccgccgg gcctcgcttt cgccgccggg
     3901 gtgatcctcc tcctgttgcc gtggtttttc cgcgcctctg cctggacggg gaacccgttc
     3961 ttcccgaaag ccaccgctct tttgggcggc gacattccgg agaacctcgc cctcttgtcg
     4021 cactgggaat cggaggcacg gggcgaaggc ggccttttgt tccgcaccct cgccctcctg
     4081 cgggaaagtc tgacgggcgt ggacgcgggc cggttcggct ttttgggccc ggtgttgctg
     4141 atgcttctgc cgttcctgcc gttcgtccgc tacggccggc ccagcgctca actgctcgga
     4201 tgcgccctgg tctcctacat tttcttcgcc gtcaccaccg gccggctgcg ctacttcatc
     4261 ccgcagatcg ggcttctctt cgccctcgcc gccgcggcca tggaggatta catcgccacc
     4321 gccgtcctgc tcgccaaaga gaggaacgtc gcgggccgca tcgccgatcg gctcggcgac
     4381 gtcctgaaat tcctggtctc cgccgtgctc ttcctgaacg ccctgtggct cgtcctcatc
     4441 ttccagaagt tcaatcaggg gtgggacgtg gtgtggggcc gccaatcgga ggcggaatat
     4501 ctccgccggg aacacaccgg ggtctacggt catccctccc agggagcctt cgatttcctc
     4561 cgtgaagccg acctctccgg gcgcgtcttc ctcgtgggcg atgcccgcac ctaccgctgc
     4621 cctctccccg cgcgggcctc cggcgctttc aatgttcccc ccttcgccct ttgggcaaaa
     4681 gaaacgccca ccccggcctc ttttatcgag cggctccgcc gggagggata cacccacctg
     4741 ctcgtcaacg tgccggaact ccggcgcatc accccctccg cctatcaggc gccggaatac
     4801 gccgcgttcc tgggccacgt gctggacagc cttccgtcgc ccctctaccg ggacccctgg
     4861 agcatcctgt tccgggtgcc ggcggccggt tgacgccatg acgccgccgg aatatctgcg
     4921 gaaaaaatcg gtgtgggcct gggcgatgta cgatttcgcc aactcggcct tcgccaccac
     4981 ggtgctggcg gtggtgttca acgtctattt cgtccagcgg gtggtgcccg ccgaaggcgt
     5041 ccgccttctg ggcgccacgg tgccgggcac gtcgttgtgg ggatacacgg tctcgctctc
     5101 catgctcatc atcttcgtct gctccccggt gttgggcgcg ctggcggatc acgccggccg
     5161 gaaaaaatcc tttctggccc ttttctggct ggcgggatgc gcggccagcg ccggcctctt
     5221 cttcgtccgg ccgggcgacg tgcggccggc gatactcctt ttcctggtgg ccaacatcgg
     5281 ctttgcgggc ggcaacgtct tctacaacgc ttttttgagc gagatcgcgg agcccgggcg
     5341 gatggggcgc gtgtcgggtt tcggctgggc cgtcgggtac ctcggcggcg gactctgtct
     5401 ggccctgaac ctgtggatga tccagtcccc gcaaaccttc gggctgtcgt ccgaagatta
     5461 tttgccggtg cgggccacgc tcctctcggt gggtgtttgg tggttcgtct tcggcctgcc
     5521 tttgatgttc gtcttaagag agcgccccgg acgcgcgacg gaacatccgg ccggactcct
     5581 ccgccccgcc gtcctccggc tgagggagac gttccaaaac ctccgccggt acaaaaacct
     5641 cttgacgttc ctggcggcct acctgctcta caacgacggc atcgagacca tcatcctgat
     5701 ggcgtcggtg ttcggcgcgc aagcgctggg catggcgcaa aacgaattga tcctctgttt
     5761 cctcatgatc caggcggtgg cgttcgcggg ggcgctgttc ttcggttggc tggcggatcg
     5821 actgtcccac aaaaaaagta tcttgtgcac gctcgccgtc tacggcgggg tgctcctgtg
     5881 ggccgcggcg atgcgcgccc ggtgggagtt ctggattttg ggagcggtgg tgggcgtggt
     5941 cctgggggga agccaggcgg cgagccggtc ctggatggcc ctcctcacgc cgccggaaaa
     6001 aagcgccgag ttcttcgcct tctacggcgt ggtgggcaaa ctcaccgcgg tgctgggccc
     6061 cctggtgttc ggcgtggtga gccaggtgtg gggcttgaga ccgggcgtcc tctcgctctt
     6121 ggttttcttc ctcgcggggg gggccctcct gacccgcgtg cgagaaaaaa gtgaatctcc
     6181 cgcctgaaga aggaacgccg gcccttccgg ggaagaactc ccgccgccgg gttcgtgcga
     6241 aaatcatcct gacgatcctc tgcggcctcg gggccgggat cctggcgtgc gaattcctgt
     6301 cccgccgttt tctgccccaa ttccgactcc cccgcctcgc cgaagaattc cgcatgggcg
     6361 gcgaccccct ctctccgaaa cgattcagcc cctccccgga cctgggctgg gaggacaggg
     6421 acgcctctcc tccacccgat gcgttcggct ttcgctcgct ccattcggcg acgaccgcca
     6481 aagcgcccaa cgaggtccgg attctcgtcc tgggcgactc ggtcggaaaa cagatcttct
     6541 cgtgcccggc ggactacgaa caggccgaag gggaactctc cctcgcctgg ggcaaacccg
     6601 tcaaactctg gagcttcgcc gtcgaagggt acaacctcga ccaatacgaa cggatcctgc
     6661 ggtatcgggc cgcctcggta tcggcggacc aggtgctgat ccttttctgc ctgaacgacg
     6721 tcgcccttca attcgtcccg gtggtgttcg aacacgaggg gcggctgctc atgctgcagg
     6781 gcatcgtcaa gtcgccgccg cgggccgtct cgccgttcct cttccgacac tcgcatctct
     6841 accgggccgg ctggacgttc cgcaacgctt atctttaccg gaagcgtttc atgcccgccc
     6901 agacctttga ttcgttcctg gaagagagac accgcgcctt tcagaacacc gtcgcttggt
     6961 gccggcaacg ggagatcgac ttttcggcgg tcatcttccc ctacttcaaa ccccgcgacc
     7021 gctacgaccg ggaagaacgg gcctcttacg cctccctgcg ggactggctg tccgccgccc
     7081 aggcccccgt tctggacctc cacgacaaat tctccgtcga cgacctgcgc gccgcccaca
     7141 atcaggaccc cacggacgac gtccatccca acccgtccgg atatcggaag gcccgcgaat
     7201 tcatcctggc cttcctcaag gcgcatcctc tctcgaacaa atgacgtccg tgtccccgcc
     7261 tatccttgcc gaaggcgatt ttgttatcat ggaacccctc tcatgtcgac cttgaacaac
     7321 cgctcccatg cctgaacgtt acgacctcct cgtggtgggg gcggggcacg ccgccatcga
     7381 ggcggccctg gccggggcgc gctccgggtt ctccaccctg ttggtgacgc tggacctctc
     7441 caaaatcggc cagatgtcct gcaaccccgc catcggcggc gtggggaaag gccaggtggt
     7501 gcgggagatc gacgccctgg ggggggagat ggccaaagcc acagaccgcg ccggggtgca
     7561 gttcaagatg ttgaaccggg gcaagggggc ggcggtgtgg agtccccggg cccagtgcga
     7621 ccggaggctc taccgggagg tgatgaccga caccctgcgg cgccggcccg gcctgaccct
     7681 gctggaggac gaggcgaccg cggtgggcgt ccggaacggc cgggttgagg acattttgac
     7741 cacaaaacac ggccggatcg gcgggaatgt ggtcattttg tccactggca ccttcctgcg
     7801 cgggctgctc caccggggcc ttcaggccac ccccggcggc cgcctgggcg agcggccttc
     7861 cgtgggcctt tccgacagcc tccgctccct cggcttcgac gtcggccggc tcaagaccgg
     7921 cacccccatg cgactggacg accgcaccat cgaccacgcc cgctgcaccc gagcccccgg
     7981 ggacgagccc cccgagccca tgtcccattt cacgcgctcc ctgccgcaac cccagctccc
     8041 ctgctggctc acccgcacca ccgccgaaac ccacgacatc atccgccgaa acctggatcg
     8101 ttcccccctc tacaccggca agatccaatc catcggaccg cgttactgcc cttccatcga
     8161 agacaaggtg gtgaagttcc cccaccacgg gtcccaccac atcttcctgg agccggaggg
     8221 gtatcacacg caggaaatct atcccaacgg gctctccacc agtctgccgg aagacgtcca
     8281 ggaacagatc gtgcacagca tccccggcct ggagaaagcc gcgttcctcc gctacggcta
     8341 cgccgtggaa tacgattact gcccccccac ccagttgaaa ccgaccctgg agaccaaggc
     8401 ggtggagggc ctctacttcg ccggccagat caacggcacc acgggctacg aggaagccgc
     8461 cgggcaggga ctgatcgccg gcatcaacgc ggtgctgaaa ctgcgcaacg agccgccctt
     8521 gattctccgg cgcgacgagg cctacctcgg cgtgatgatc gacgacctgg tcaccaaggg
     8581 caccgacgaa ccttaccggc tcatgacctc ccgggcggaa taccgcctgg tgctccgctg
     8641 ggacaacgcc gacctgcggc tgatggatta cggccggcgc gtgggcctca tcccgccgga
     8701 cgtgcacgcg catttcgtcg actatcgccg ccgcgtgcgg gacgccgtgc gcgcggccct
     8761 gccgccggac gctctcgagg aaccggccgc cccccccacc gatgaatcgc tcctcccttt
     8821 tccagaagcc gccgaccgcc ctcccgcaag cgccgaccgc aatttctggg acaaacggca
     8881 cattcaacgc caggtggcga tcgagacgac gtactggggc tacatcaaac ggcaaaaaca
     8941 ggaactggcc aagttccgca agtcggaatc ccggcgcatt cctccggagt tcgactacga
     9001 cgccatcaaa agcctgctga ccgagtcgcg gcagaaactg aagaaagtgc ggcctgaatc
     9061 gctcggccag gccgcgcgca tcccgggcgt cacccccgcc gacatcgccg tgctcctggt
     9121 gcacctggaa cgacggcggc gcgcccgccg ccaaggcgac acggcgaccc gcgcctcctg
     9181 acccttcctt ccatgcaaaa accgttcgac ttcgagaccc atctcacgct ggaggccgcc
     9241 ttccagaaat gggaaccgct tttcgccgaa acgggcgtgg aactcggctc cgcggccgga
     9301 aggctcatga gccacttcct caaggaactc aaggtctgga accggcgggt gaacctcgtc
     9361 tcctttcatt gcgacgagga actcctgctg gggcacctgc tggattccct ggcggcgctc
     9421 caggacccct ggctgaaaaa ccagccctcc cttcgatttg tggatgtcgg gaccgggggc
     9481 ggtttccccg gactgcccct cgtcctcgcg cggccggaat ggcgcgggct ccttctggaa
     9541 tcggtgcgta aaaaaacggc gttcctggag accctggtcg aacgtctgcc gctgcccacc
     9601 gtcgccgtgt tggcggaacg ggccgaggac gcgggccgcc gggaagaaca ccgggaggaa
     9661 tacgacgccg ccttctgccg ggcggtcgga cctttttcca ccgtcctgga attgacgctg
     9721 ccgctggtgc gcgtgggcgg cgccgcgctc ctccaccggg gccacgaggc cccgcaggag
     9781 acctccgccg ccggggaagc cctcgcccgg ctgggcggcc ggctgggcgg gcacacggtc
     9841 tatcgcctgc cgcatctgac ccagccgcgc tacatcgtgc gaatcgaaaa gacgtcggcc
     9901 acgccggagg agtatccccg ccgcaacggc cggccggcca agcggcctct ctgagcccgc
     9961 ggtgtttgtg aaattttccc catgaaaaaa cctctcctgc cggccctcct cctgtcggcc
    10021 gccgtcggcg gggcggcgtt gtggcatttc acccggctgc ctctccacga gaaagggaaa
    10081 aaactcctgg aacagggcga cgtcaccggg gcggtggaac tcttccgacg cgccctcgaa
    10141 gaaaaaacct gggcgccgcc ggtggagcgg gagatccgga acgaactgtc ccgcgcctat
    10201 ctcctgaagg gcgccgtgga caacgccgag gaggaattcc agaaaaccct ggaacgtttt
    10261 ccggaaaacg cctacgccca catcgggatg gggttcctcc agttggccaa agggttcgac
    10321 aacttcgcgt tggaatcctt ccaaaaagcg aaagcggccg acccggacga tttgcgggcc
    10381 tccctcggcc tggcgagcct ctacgcctat cgcggcgagc tgcccgcggc gcgcaaagaa
    10441 tacgacgagc tcgtggcgcg ggcgccggag aaccgggcca tccgcgaggg atccggcgac
    10501 gtgttcttcg gggaggggct gttcaacgag gcggccgcgg aatacgagcg cgccctggcg
    10561 gacctgccgg aaaattccac cacgcggctc gcggcggcgc gcgccctctt ggcggcggga
    10621 cgatgcgatg ccgccgagga actgacgcgc cgtctggtcg ccgggtcccc gcgcgcctcg
    10681 gcggcgcggc tgctcctggc ggacatctat cgggacaccg ggcgcttcgc cgacgccggc
    10741 ccggtgtatc aggcccttct gagcgaagac caacgaaacg ttttcgcctt gctccgggac
    10801 gcggagtggc aggcgcgacg cgatgatttc gagacggcca aacgacggct cgaccaggcc
    10861 gcccaggtca tgcccaaacc gggggaggcc ccggtctctt tcgccagtct cgccgagatt
    10921 gaggatttcc tggaggtacg gagctacctc cgtcaggcgg ccgtcgaaac gggcctggcc
    10981 tggtccaaat tttacgcgta tcagggccac tacgccgacg ccgaacgcga agccctgcgg
    11041 gccctccagc tcttccagaa acacctggcc gccctccgct ggatggcgga actgcagcgg
    11101 atccggggag acggcccgaa acgcctggaa tggatccggc gcgcccgcgg gttcttcccc
    11161 cgccacgcgt tgcttctgtc ggacgaggcg gaggcgctgt tggccttgaa aaaaatcgcc
    11221 gaggcccggc ccccggcgga ggaggcccga cgctcgtgtc caacccttcc gcgcgccgcc
    11281 gcggttctgg ctcaggcgcg gctggccgaa ggaaaaattt ccgaagcggc ctccgccgcg
    11341 gcggaggccg tggccctcgg cccgcgggac gcccaggcgc atctggcgat ggccctggtc
    11401 ctgcgatcgc agaaaaaata cgccgaggcc gaaaccgctc tccgccgttc tctggaactg
    11461 gacccctaca acgcccgcgc ccacgacgaa cgggcgcagg tcctctccct cctcaagaaa
    11521 ccgaccgact ccgtcatcgc cggccgggaa gccgcccgac tggaaccaac gacttacccg
    11581 ctccgaaaaa actaaaaccg tgatccccat ccgcgacagc gtcaaaagcc gttcctaccc
    11641 gctcgtcaac gtactgctca tcgccgccaa cctggcgatg tttgtccggg agatcacact
    11701 gtcgaatgcg gacgttttac atgatttcat ctctgcctgg gcggtcgtcc cgggccgcct
    11761 ggtggcgttt ccctggacgg actggcccac gatcttttcc gccatgttcc tgcacggcgg
    11821 ctggagccat gtgctgggca acatgatgta cctttggatc ttcggcgaca acgtggaaga
    11881 ccgcctgggt catttccggt atctcgtgtt ctatctctgc gcgggaacgg cggccaccct
    11941 ggcccaggtg tttctccacc cgctcagccg cgtgccgatg atcggggcca gcggcgccat
    12001 cgccggcgtt ctcggcgcct atttcgtcct ctatcccggc gccaaagtgc tgacgctaat
    12061 ccccctcggc tttttcgttc gcctggtgga gattccggct tttttcttcc tcggattttg
    12121 gttcgtgttg caggcgtttc aggggtgggg gtcgctgctg cagagcgccg cgcacgggga
    12181 gacgggcggg gtggcgtggt gggcgcacgc cggcggattt tttgcggggt tcgtcctggt
    12241 gtggttcttc aagcgttccg gacgacggaa gagatagggc gcttcgtcac tccagcggga
    12301 tcttcagcgc ctccgatatc ttcttcctgt cccgccgacg gtaaatctcc gtcacccgtc
    12361 cgtcgagacg cgcgtattcc tccaaaatct tccggccgtt tgccacccgc tcatccgacg
    12421 ccacggcgtc ccacaccacg ccatcgtgga ggacattcgc cagcgccagg aacaacatgg
    12481 cctggtccag gaccagatac ccggccgccg cccggcttgt tttccagtcg aacgaatccc
    12541 ggaacccgaa acgatattcc cggccgtttt ccacataggg cgcccgcgcg ccgagccggt
    12601 ccagcgcccg caggttggcc gtggcccggc ggggataata ggacatcgcc aaaaccgagg
    12661 cgtggggtgt gaccacctcg tccttgagcg cgccccaacc caggtattcg cccgtggggg
    12721 ccgccgccgc cgaccatccc cacgccggcg cggcgatgcg ctccgcgtgg aggatctgcg
    12781 cgtaggcgaa attcgccgcc gatttcccca gcaccgtcga ccgctcgtcg aagaaaagcc
    12841 ccgacacgca ttgcatgaaa agtccgccgc cctgccagcc ggggacgaaa taatcctgcc
    12901 cggaacgcgt ctcccgggac ctgtccagcg atttccagtg gatcagcggc acctggcccg
    12961 tggccacgcc gaagaaactc cccagccgcg tgtccgcccc cagataaccg taataccaat
    13021 tgccccacgg ctggcggatg tcgaaatctt tcgtgtcgag acggtacccg ccgcggtaga
    13081 gatggttggc cggttcatag aggatccccc agttcatcgg ggccaggagc gcgtccgcct
    13141 tcggccgcag ttccgggaac gcgcggcgaa ccagcaccag gccggcggtg aggttcccca
    13201 gatggtccac ggtggaaaat tgggtctcgg tggtctgcag ggtgtcggcg ttgaaccagg
    13261 tgacgggaaa cccgcgccat ttctccacgc gggccagccc gtccaagact ttttcgatcc
    13321 gccggagggc gtcctcctcg gaaaggagcc cggttttctg ggccgccgcg caggcggcca
    13381 tgtagaaacc ggcgttggtg agggaggtgc tgggaagccg ttcgtgggaa tcgtagggca
    13441 tgcccgtcgt cgggctgacg aaatgggcga ggcagcgcca ggtgtccgcg tacacgcccg
    13501 cgagatattt ccgatcggaa cgcgagaggg gcgcggccag gagcggtccc ggtaaaaaaa
    13561 aggcgagggc ccatgtcaga caacgtcgtc gcatgggggg atattaccaa aaaaccgtca
    13621 ggcttggatt tggagaagtt gaatgtgtcg gaattccgct tccatgtccg aggaggaccg
    13681 gaagatgcgg aactccattt cgctgacgac ttcgatgagg agggccttgg cggcggaacg
    13741 ccagtctttc ttccagcgct ccacgtcgtc ggtgaatatc tcggcgcggg ccggcacgcc
    13801 cagccgtttc tccagagtct tcaacacccg gtccgggtcc atgagacctt cgaacccgga
    13861 gcgcacctcg ccgcccgaaa gcacgatcac gttgtcgcgc agaacgtccc acaggtcgga
    13921 ggggatctgc gtgcggagga gttccaacaa atctttttct ttcaccgaac cgttcgacag
    13981 cacggccaag ggcgcggtcg tctcctggga agacgcgcgc cggaccagct cctcctgcag
    14041 ggaacggacc agccgcattt cgcgcgggga gagcggttcg cggttccgca acgtctccgc
    14101 gagtccgctg agatcgtagg atttcaccac cggctgggcg ggcacctcgg cttcccaagc
    14161 ctggaggaac cggctcagcg cttttatgcg ccacggatgt cggccctgct gggcccaacg
    14221 cgaggagacc cagccctttt gatacgcggc ctccaggcgg cgcgcctcgt cgccggtgag
    14281 aggccggcgg gacttcatca ggtcttttat cttctgttcc agcgccgtct cccggtccga
    14341 cgccccggcc ggaagcccct tgccaaacgc catcaaaagc cgggcgagcg cgagcgcctg
    14401 ccgggaatcc gccggatccg gtccggaggt tttggccggc cgcacgccga accgtttgag
    14461 gaaatctctc tggatcggcg gcaggaggtc gaattcattt tcgaacaaaa cccgcaagag
    14521 cgcccgaacc tcctccgttt gcggcaggtc ggccgttccc gccgacgggt tcaaggtctg
    14581 atgcatggca tgaagcgccc ccagcgcctc cagggaggcg gcgccgatgt ctccgcgcgc
    14641 cgaggcggaa gaggccgccg tcttctcgag gcgttcgccg agggcccggg ccatcttttc
    14701 ggaaaacccg gaggccgccc ggtccatctc cgccgccgga agatatcgga attcccgcaa
    14761 ctccgaggac agaagaccct cggaaccatt cccccagcgg gccgccacgg acgaaagccg
    14821 acgcccgacg atccggcgca aggacggccg aacggcgcgg gaaacgccgt tcaccggcgg
    14881 aagcaccgcg ggcaaaagcg gcaaggtccg ttcccggcgg cgctgggccg acgccgcccg
    14941 ttggggagcg gggtgaaccc gggcgaacgt ggcgatcacg ccggcgagga actcgaattt
    15001 ttccggcagc ggcgccggat cgtccaactc ggcgaaggcc ttgccgaaga gcgcctccag
    15061 gcgggaatct ttcaacgccg cggcgagccg aagccttcgg agcaggtcgc cgagggattc
    15121 gaaaccccgg cgagtcccgg tttccatcca ccgggcgaac accgccgcca acacctgccg
    15181 ggcttcctgt tcccggccga gttgcgcggc cgccgccaga tacgtctctt ccagcggagg
    15241 gaggccgggg gctttgtcca aggtcgccga catcgtcctg gaaacatcct cgaaaagctc
    15301 tcccgcccgc gccgcgttcc cctgtttccc ataagccaac accagcgcca gaccgaaacg
    15361 cgacgaaatt tcgccggcgg gcaaagtccc ccgcagatcg tccagttcac ccaggagagc
    15421 cgcgttttcg gaagacgcgg acagccgtgg aagcgcctcg aacagggacg ccagaacctc
    15481 ggcgtcggtt ttttccaaat caacggcccg gaacgccttc gcgagcccct ccgccagagg
    15541 acgcccagaa acctccttca gcccggccgc caaagcgaaa tccatcaaag cgggattgga
    15601 cgccgccagg cgcaggaaag cctcccgata gaacgccgtc tttcgacggg aaccgagttt
    15661 ctcaaaaagt cccatcagtc cccagcggag gcgcgctttt tccatcggat cggccgcctg
    15721 cgtcaggagc cgttccactt ccagggccag ggccttggcg gcttgcggct gaccggcggt
    15781 gacgtacgtc tccgccagcc gcaccaacgc cgctcctttt ttctccggcc gcgccgacca
    15841 ccagggagag gcccggaggt ggttggccca cgcggtcagc ggttccaagg tgctgcgcca
    15901 ttttttctcc tgacgaaaat cggccgcaag atcgaaggtc caaaggcgca catcctctct
    15961 cgtgaccggc cgatgggcct gcgcgcgttc cagggcgcgg gcgtggatac cgaactgcaa
    16021 ccgcgtctgg gtgcggaagg attcctccat cctcccgccg ccgcgccatt cgtgggaaag
    16081 ccattttcgg tagagattct cgttgatgat caggtggccg tcgctcgtcc aaacggcgtt
    16141 gatttccttg gccaagccgg cctcctcatg cccgggcatg ttgaactgca tcacggaaac
    16201 ggtgagcggc ccttgtcccg gcaggaggcc ctcgtcccac aggtcccgaa ggacccgttc
    16261 gcccgtttcc gccaagggct gcaactccgg aggaaccacc gccagagacc ggccgttctc
    16321 caacaccggc gttaaacgga tatctttcga ctccaaataa acccgatagt cctcgagggt
    16381 tcccaggaat cccggcgggg tcagcggcgg tccgtctcgc cggcggcggc gagcgcccaa
    16441 cgcggaaagc atcccggcca aaggaaccgc gggaacggaa acccccggcg ccgcgcggtc
    16501 gccgaaaaag gctccggtcc gtcgaccgtc ctcttctttg gcctcgtaat gcctgttctc
    16561 gaaacggcgg gcttcctcct tgagtttttc gaggtcttta aggaactgtt ccctctcggc
    16621 cggcgcgaac tgcggcgtga ccacgctccc ggtgtcgttc cggaaaacga gcggaagcgg
    16681 cgccgccgca ccgtcggggc cgtaataccg gatctcgctg aatttcccgt ccttgcgacc
    16741 ggtgaaaccg atgaaggtgc cgtacggcac gagatcggac gcctccagag gggaggcccc
    16801 cccgcaggcc atgtagcgtc ccatgccgtc ctgcacgttg attttatcga cacccgaccg
    16861 aagaagttcc tggcgccgct gagggttgtt ccacacagcg gcgaacaact tgaccgcgcg
    16921 gggctcgcgc atctcgatgg aattcggcaa caccacgaag tagacgtttt cctggccggc
    16981 cgatttgacg atccggggct cggcccccat cgtcgcctga tattgggccc actcctgctc
    17041 gaaccgctca agcgcctgcg ccacctgcgc ctccgtcaac gtcgaccggg cctcccagag
    17101 tttcctcaag aaatgttcct gcatcccctc ataaatctgc gactgctgaa gcatcaccac
    17161 cgccacgccg ggacgttgtt cgtcgaactg ggaatggatg gtcggcggcg gcaccttgta
    17221 gcggccggcg gcgttgaaca ccgccggata ccagtcgatg accgcggtga tgtggttctt
    17281 cccggcatac accacggcca gccccttgtc ggccttcttc agcggctggg ggaacgtggt
    17341 cacgcccggt ccgttgcccg tcacaaaaat gacttccgct ccgagttcct ttatcaaccc
    17401 ggcgcgggga ttgtcgtcgt tcaggaacag atgcgcctct cctccgccct gttcctccag
    17461 gtaatgtctt atggggattt cccgccccac gtcgcccatc agttccgcgg cgaaccgata
    17521 ttcgttcagg tgcccgtgcg tttcgccgat gcccaaaacc ggtttctggc cccgggcctg
    17581 cgcctctttc aggagagccg tcacttcgcc gcggaactcc gcataggttt ttctcggcga
    17641 ctgctcgaaa ttcctggccc aaccaaacgt ctcggaaacg agcgtctcca cctcgggggc
    17701 ggccgcctgc accgccggaa gcgtcacggt cgtcggcgtc gggggcggga cgtcgggggc
    17761 ggcggccagg gacaggcgcg cggtggcggc gttcagggcg gccgccagag tcgcgaacac
    17821 catcacgcgg cggaacagcg gctgcggcga atagaattgg tctttggaga gcggccaccg
    17881 ccaccacatc agcccgcgga tgagcgtgac cgcggaggtt ttggcgtagg cgctcagttc
    17941 ggtccggaaa taacggcttt tcagaaaaga attgaccgcg aagaggggcc gggtcaaccg
    18001 gttcacggga agggatgcca gcggcaggga ccagccgaga aggtgcgtct cctcgtggga
    18061 gaggatcgcg ggcaggatga actgccggag ccggttcgac tgcccttcca ggagccagcc
    18121 gatgtttatg tagaggacgg gccggccctc ttcccggcgg gcctcggcga cggcgcgttc
    18181 gtctccgaac gtttcccaca tctcccgggt ggtccggacg cggatttgtt gtccccaaat
    18241 gtccgtcaac gccgtttcca tctgggcgcg caactcctcc gtcgacagat tcgccaacgt
    18301 ggggaccccc agcttttcca gaacactctt gaaagcggcc cgtttctggg acgttccgaa
    18361 actgccggcc cgcagggctt tttgtccggc ccggcggttc agcagaacgg cgaacccgtg
    18421 ccagagggag gccgccccca agaagccggc gatcaccggc gacccgaacg ccgtcagggc
    18481 ggccaaaagg agccccccgg cggaaccgag gaccgcataa cgggcggcct gtttcaggtg
    18541 aaactggccc cgggcggatg tcggaatctt ttcaaaaatc tgtttgagag tctctagaaa
    18601 acagggcgaa tactggtccg gttctccgcc gaggatttta aaagcgagtt ccctgctcat
    18661 gggcacattg taggaacgac ggctggtcat ggcgtcgaac gcgtccgcga tcgtcagcag
    18721 gcggtcgatc ggcgggatct ccgacccgga caaacgtctg ttcctcccct tcaacggaac
    18781 gcgcgggtat cccttcccgt cgacacgctg gtgatggagg gtcccgagcc acacgagact
    18841 gttcggaaac cgttcccgca gaaacaccaa gcctttgatc gcgtgcgtgc gcaaaatgtc
    18901 catctcgttg tccgtcagtt tgggtttgct gtcgtccaaa agggccgcgg gaatgcccaa
    18961 tttgccgata tcgtgcagca gacccgcctg ccggagaaat tcgttctgtt ccggcgaata
    19021 acccaaccgg cggccggcct ggcggacata gctggccacc cggcccgaat gatggacgga
    19081 cgtcgggtgg tgggctttca gttcggccaa caggccgttg attttttcga tcggcaagaa
    19141 acccgcggaa aggtcccggt ccctttcgaa tttttcctga acgaacggca gggacagcag
    19201 ggccgccgcg gaactcagcg aaaggccgag ggacatcgcc tcccgccgcc acgcctcgaa
    19261 attttccgcc gtgggggcgc ggcccgcgtt ccgttcccgg aattccgacc ggcgccgcag
    19321 gagatggtcc ttctgcagga gcgcgatcag gaggaccgcc cgcacgtcga gccccttgcg
    19381 gcgggcctcc tcgaaaaatc ggtcgtcgaa gaacaccgtt ccgcggctgt tggcccgcac
    19441 gtggccgtcc atttcgggca agacggccgc cggcgggccc cgggacatcg cgagccgcgt
    19501 tatcgcggaa tccggcaaca actcatggaa cgccgtcaac gcctccgaca attcttcggg
    19561 ggatatccaa cgatggagcc ccgtgtccac cacgggcggc tcggcccgga acggcagggg
    19621 ccgatggagc cggtaatccc cccggagcgt cacgtcgggg gactccaccg tctcttccgt
    19681 ctgccgctga ttcaaatggt agacccgggc ctcggggaac cgctccctca gggtttcgac
    19741 gttgggcgaa agatcgtcga acgccgccac cacgcgcccg agcttggcga tctcgtcgcg
    19801 gctcgccgtt ttgaaggcga acccgtacgg gtcgttgtcc ctgaactccg gggaacgcat
    19861 caggagggaa acgcgccggt cgtcctcggg gttcggcacg ccgaatcgcc gcaattgggc
    19921 catggtgagg gagcgctctt ccgcgtcgaa acgcccggtg agaaacacga tgcgggcgcc
    19981 ccgggcatgc agttcccgca caaaacggac ggcgccgggc aggggccggt cgtgcacgaa
    20041 attttcgggc aggtattgac gcgcccccgc cgcctttcgt tcctcctccg tgagccccaa
    20101 caccgcggcc gcccggtcca ggaggacgga ccagggcccg gtcatatcct ccaaacgcca
    20161 ggcaaggacc ttccggggtc cggagagatc cgcgccgggg cgctgcatcc gttcacgggc
    20221 gagaccgtgc aggatggcga ggctgcgggg gcgcgtgtcg aacagcgtgt cgtcgaggtc
    20281 gaacacgacg accggctgtt cgcctttctt cacagactcc tccaccgccg aaagcaccgg
    20341 cttcagtttt tcctgttcgg ccggcgggtc gtacgggtcc aggagaggcg acatcagccg
    20401 gtccaccgcc ggatcctcgc gcagggtcgg caggcttccg atgacgatgg gctgggggcc
    20461 ggctttcagg acattttcca gccccgcaaa atgacggggg ttcactcggg agggggcgtt
    20521 cagctcggcg agttccgccg gagtgagttt cttccagccg gaccggccgc gcaatatctc
    20581 caggcggctc accagggaaa accggtccac gcggagagtc cggaggttgt cccccggccg
    20641 gttccacacg cgggtgcgga tgtaatagtc gccgcgccga ctctgccaca catggagcgg
    20701 tctccctcga cggaaaacga ggtccggctt gtccggatgt tcggtgaggg cggcgtgata
    20761 gcgccagcgg atcaggtggt ccttgggcat gttgctccgt acgccctcca gcgccccggc
    20821 ggtgctgcgc accgtggaaa cgatgacgac cttgtgggaa tccttcgacg ggaaattcga
    20881 caggtaccgc atttccggaa ccggcgacct cccgaaaacc cggcggatga ggctgagctg
    20941 gccgggatag gggctggtga tcacgatgtc ttcgagccgc aggccgcgcg ccaaaaggtc
    21001 ctgaacggtc ttgagaactt cccgcgcctc ggtgaggttg ctgtagtccg aaccgtcttc
    21061 cttcagcttc gtttcgaaat ggggtccgtc gggaagctcc gagaggtcga ccacgcgggc
    21121 ggtggaagcg gcggaaagat tgaaatgtct tgagacgcgg tcgtggagga cgcgctgcca
    21181 ttcgcgggtg atgacgacgc cggcggcctg ggcggagagc gtctcgtgcc gttccatgac
    21241 ctcgtgcaac agcgtctgca cgagcgcctt gacggggtcc ttcccctgcc gcacccagcg
    21301 ctcaacggtc ctttccggaa cgaggaaatg cccgtcctgg gtccacgtgg gaaccagtcc
    21361 cggcgggccg cgcgtcaaat gcacgttcaa aagcgagagg cgcagcccgt aaacttcctt
    21421 caacatcttc cgggcctgcc ggaactccct ctggaacggg ttggccgtcg aaggaacgac
    21481 gaccggtccc tccaggccgc cggcggaagc cgccgcttcc aggagccgca cggtcaaccc
    21541 gtaaggcaaa aggaacgccc gcccggacac tttcaaggcc gccgccaaac cgtccaggtc
    21601 gcccgttctc acatccggaa tgccgagatg cgtgaccagc tccgtccaaa gcttgttcgc
    21661 cgtctccgtg gacgggaggg agcggaccca cgactccaac aaaggccacc aggccatgac
    21721 cccctcgacg gaaggcccct ccagcgcctt ctttttctgc gcggcggcgc cgggggaccg
    21781 gttgtacttt tcgaaagcct cggccatgct cagcccgccg tcctccggca aggcggaaac
    21841 ggcgttcacg aaatcacgta tcgtgttgcg gtccccccgg caccagtagt agatcatccc
    21901 atagacccgg gccgccatct cgtaggaatc cggatggtag accagattga ggccgccccc
    21961 ccgcagcaac cgggcgaact cctcccattg tttgacgggc tcgttgcgga acttcttcat
    22021 gatcttcgct tcggtgaaag gttccacctc gccctcccaa accgagaaat acagggcctg
    22081 ggcttccttg ccggtcttca ctttccgggc ggcctggatc ccccgcaaga catccagcaa
    22141 ccgaagttcc aacaccttga ccgcgcgcat cctcacccgg acgtccaaat ccagtttgcg
    22201 gctgctgtcg aaccgttttg tgagccggtc cgcgaccttc tccggttttt tcccggcgaa
    22261 ggcgtcccgc accgcggcgt cacgcagcgc cgggttttca ctggccaaca ccgtgtgaag
    22321 gacgcgcccg ccctcatagg ccgcggcgag caaggaaagg tcctcatgga agtccgtccg
    22381 cgcctcccgg acgtccttca aaagttcgtc gcgcctcttc atggatatcg ggatcgtcac
    22441 ggtcacgccg tcttccctca ccgagagaca gcccgccagg tccttgaaca tgttgatctg
    22501 gtgccgtccc tcgtgggcca cgcgggccag ctcgtaatgg aaaaacgccg tcccattgag
    22561 aaaagtgctg agggggtgga cgacgacggc gttggcctcc ttggtggagg cggcgttcag
    22621 gtatttgtag atgaacaccg tcaggttgag catgtattcc cgcagttccg cccgattctg
    22681 cggcgaaccg gcctggcggt cccggagcat ttcggtcaac gcgagacggc acatggcccg
    22741 ctgatagagg agccgggcga tgctgttgtt ccacggatcg agggcttccg cccgtttctg
    22801 gatcccctcc aaccgcgagg cgatgtcctc cagggagagg gtgtccgccg cgagcccggc
    22861 cagcggggcc tccagttcgg acaagagcac gagccatgag ggcccgatgt attcccgcag
    22921 acggttctga aaaacggtct tcgccgacga ccgcgggtcg agatagtcga ccagcaggtc
    22981 gtgtttcaag ccccagagga acgccatgac gaaccggtcg tttcggctca acaacgcgaa
    23041 agagtctccg gcgatttgat ccggggcgga cgatccggcg gtcacaagcg cggccgccgt
    23101 ccgcccgaca acccccggca gaacaacgtc ctgccgggaa atgctccgct ccatctgccc
    23161 gagaagggcc ggcagtaatt tctcgaacgg ttccctggaa acggccccct ccgcttggcc
    23221 gtagaacgtg aagaaatcgg tcatctgcag tatctcctcg aaagcgccgg gaacgaaatc
    23281 ctccaggtgc gcggcgttct ccagggcgaa acgcagttct tcgaaaatat cccccatctt
    23341 ttccagacgg taatccgacg acacccgaac gctccgcgag agctcgtccc gaaaaacctc
    23401 cctcaatttc tcgaagcggg ccaggagcgc tttgtcctcc tcggtcgacg gcggcgacac
    23461 cggtttcggt tgaacgggcg acggcgtggc ggcccaggcg aaacgattcg aggcccagag
    23521 cccgacgagc gccatggagg tgccgcggag gaaatcccgg cgggtcatgg ccgcggcggc
    23581 cgccagccac ggcgccacca ggctgcccaa cgccacggcg ccggaaagat aaaggccctg
    23641 cccggccgcg cccgacgttc gggagagcgc cgatttgatc cggcgtttcc attgccgcat
    23701 cggcgtgtcc ggctgaaggc tgtgaaaaac ggaagcctcc cccgcctgga gcaatccccg
    23761 gtcctgaaga taggacgtca ccagacccgg aagacggttg gatatccagt tctccacgtg
    23821 ggacgttttc agcgtggccg gaacaatggg gttgtccacg acgtcctgct gaaagtggtt
    23881 cgccaggtcc gcggccaggg cctcgaattg ttccggcgtc acctgggccg ccagcgcgtt
    23941 gatccgccgg gaataggaga cgatgtcctt cttctgcgag atgtcgtaat ccaacgagag
    24001 gagcgcgctg aaaaacgccc ggctcatcag cgcccgtcgc tccgcggggt cggcaaaagc
    24061 ctctccgcgc atttcccgat ggagaaggat ttcatacggc agatattcgt aaccctccgg
    24121 gatgtcggtc acgatgcctt ttttcccggc gggcagtttt tggcggatgt tgccgtaatg
    24181 ggccgaatgg acgaacccgc gcaccacccg tatccgggac tggggggcga gcttgtttat
    24241 ctgtctcagc cgaacgtgca gcagggcatc gcgttccttc tcatattccg ccatgcgcgt
    24301 cagatgcgtc tcaaaaaacc gcatggcgcc ggcggaatcc ccctccagca gacgcagcaa
    24361 cgcctggttg agcgcctcct gtgccgactg ttccaacttc agaatggtcg gaggcaacgg
    24421 cggatcgccc tcgatccgga cccggcttcc gtaaagtttc accagcgttc ccagaacggc
    24481 ggattggaag tacgaggtgt atcccgattc ctgcccgatg agttccaccg ctttttccgg
    24541 cgacaggcgc ccgagagaaa cggcctggta ataggggacc cattcgtgcg gtatttccag
    24601 gaaaaggatg tcggcgtccc gcagggcgga ttcgaccact ttggccgtca gccccggaac
    24661 gtcgtgtttg tcgaaaagat agacggtgtc ctgacgggcg cgatccagga acatgaggcg
    24721 gctccgccgg agaatgcccc gggccgccat ggcgaggttg gcgaacaggt gcgtgtggaa
    24781 gaggggcagc gaaaagaatt ttcggttcag gggaaggagg aaccatcggt tcaaccacgg
    24841 ggcgaaaggg agacgcgtgg gcagcgtggg caaacgaacg ccttccaggt ggacatgctc
    24901 gtgcgccatc acgatgggca agatgaaccg ctggaggcgc gcggaccggt ccgcggccca
    24961 gccctggttc aaatagatgg tccccgggac ctccggatgg acgaaagcca ccgtgttttc
    25021 atcgccggac agggccgcca tccgttcggt ggaaaggagt tcgacggtcg tcccgtaatc
    25081 cacgctcaac tgatattccc atcgggcctt ctcctcaaac ggcatcgccg aacggccgag
    25141 ttcgacgacg cgccggccgg cctggtggga ccaccgcaac acgatcccgt tccagaagcc
    25201 gagggccgac aggaagacca gcggcacgcc cccgagaggc gcgatgaaca acataacgat
    25261 agcggcggcg ccggccgctc ccgacgccgc ataggcgatg ttacgaaaac gctggaaagc
    25321 ctccaggttc atcgcctgaa cgcgctcctt cagcgcggtc ttgtccgtct gcttcaaaaa
    25381 tttccgcacc ttttccaggg taaactgccc ctcttccagc ggcccgaatt ttttctcgta
    25441 ggcctggcgg gccagagcct gcaacgtgtc cctgtcccac tccagaatgt ccttgaaggc
    25501 ctcgcgggcg gcgcgccagt cgcggacggg tttgccgacc aacgcctgaa acacctcctg
    25561 ggcgatggcc cggtcgcgcg cataatcctc cagcggcggc agcaggagcc gggtgaaaga
    25621 cca
//
LOCUS       DAOTFD010000018        23612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1421_length_23612_cov_4.190050, whole genome shotgun sequence.
ACCESSION   DAOTFD010000018 DAOTFD010000000
VERSION     DAOTFD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23612
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_23612_cov_4.190050"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="P5079_02245"
     CDS             <1..1265
                     /locus_tag="P5079_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY28840.1"
                     /translation="DSIDLAEDRERFGALLKRLKIPHPAHGAARTLAEAQRAAARVGY
                     PLMVRPSYVLGGRAMEVVYDDKQLAGYMDRALAGGAGLPILLDRFLEAAKEVDVDAIS
                     DGKDVFIGGIMEHIEEAGIHSGDSACVLPPYSLDEKIVETITAQTRKLAKALKVKGLL
                     NMQYAIRDGVVYVLEANPRASRTVPFVSKATGVPLAKIAAKVMAGLPLKKCLAPWRKV
                     LARRKSWYAVKEVVFPWMRFPEVDVVLGPEMRSTGEVMGIDGGVGLAFAKSQAAAGGA
                     LPKSGKILFSVREKDRPEAFRVASGLAALGYRLAGTSGTAAYLRSRGLTIESVKKLSE
                     GHPNVVDVIRDGEVSLLINTPDGPRARSDGFHIRRTALLHNVPLITTLAAAHAAVEGL
                     QQSFVSPWRIHSLQELHALPADNRTIDG"
     gene            1295..2962
                     /locus_tag="P5079_02250"
     CDS             1295..2962
                     /locus_tag="P5079_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY28841.1"
                     /translation="MTSPKGFLGFSGPVNAWRNSFHVVVLVGIVVLYAVVNYFWLQKT
                     CFVPHNPDEAFHIYTGVFNRYYPVENIGQLVSEIRKAVREPLYGLMAVVANKYFSKSL
                     FVTRYVNLFWFAIALFAVYRLGTVLYDSRAGLYSAFVMAFYPAMFGFSRIYSPEFGLI
                     GVMTFAVVFLFQSDGFFVTRSAVLFGLMLGMGLMIKNIMALFIVGPLVYAVFRHRSFK
                     YVRVRNIIVSLGIALILIIWRYSNVELLSLYLQSPFGESSGAWHEWRNLRVLTWDAVR
                     YQFSPIFFGLFFWSLSGFLKQGRKDGRAMLLLWIVIPVVFFILIPHRKVPRYVIPYLP
                     AFAVISGIGLALLERRRQRVIVSVFVVLGLVQFFLCSFPGRRDFKESGLFFCGREDWK
                     DPALCVERPCGEKCFRPIAEVIEGTSEKKNPFLVVFPDRRHFTVKWALFRWFNDVQLR
                     LALLERENLFQMEPYVKQADYLLMPAMAWGAIGDDYFDQQISLAKQHFEWPTKKNQKL
                     VDFVNGYSVAYKKRLIDEMGEFQEIGRFELSLEDDTFVLLKRKKETL"
     gene            3059..4492
                     /locus_tag="P5079_02255"
     CDS             3059..4492
                     /locus_tag="P5079_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28842.1"
                     /translation="MRIVFVFPPFDHKKFEEDIDVVSREFTQPPPLGLAYAAAIAEKS
                     GHDAIIIDANLRPRMSKEDVLAQINEFKPQILSFMLTAYMFQQTLEWIVFLKQKTGLP
                     VLVGNILMELYPKEVFSHREIDFGIIGSAQKPLPALLRALENGGSLEGIKGLCYRENG
                     QLVIQPPDTLREDFDSLPFPARHLLANDRYYTVVSKRKNFTIMMTSKGCVSRCGFCHV
                     KDIPYSARSPVGVADELEECYGRHGIREIEFFDPVFTVNRARVREICLEIRRRGLDIS
                     WACRARVDQVDEELLREMKAAGCARIYYGLECGNQAILDKTSKGITLERIREVVRITQ
                     NCGILTLGFFLIGAPGETVRTIEETIRFAMSLGLDYAQFHKTVAKPKTLLYDEVMRTT
                     GRDYWGEFVMGTAKEERLPTPWTVVSDGDIEKLTVRAYRRFYFQPSRLWKIFRGIKSF
                     SEFGRYVRSAVGIWRVKSDLHGRRIAP"
     gene            4489..6021
                     /locus_tag="P5079_02260"
     CDS             4489..6021
                     /locus_tag="P5079_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28843.1"
                     /translation="MRVMFVYGAFENLGIEYLSAVLKSHHHTTRLALDPLLFDDPFLR
                     LPPLARFFDHQKSLIREIAEFGPGLLAFSVPSCYYKKALSLANAVKRILPSVHVTFGG
                     IHPTAVPETVLENDCVDSVVLGEGEYPLLELADCLGRGRLDTAIRNIWFRQAGGIVKN
                     PVRPYISNLDELPYPDKGLYYDILPRYRKGYTVITRRGCTGGCSYCCLNVWHALYPGQ
                     PRIRIRGIENVIRELREAKERYNFTQLRINDDLFTYDKNWLRDFAKEYKRHIGTPVWC
                     FTSPAYVDEEVVRSLEKMSCRQVCLGIQSPVRALRERILHRYVDNEKNTRAIQLLKAA
                     GIRCLVDTIMGFPGETEDDLVETARYYNETRPDRICIFWLIYYPRTPLVEMAKGMGVL
                     DETQCARLECEPTHLANTIPDRRRYLRKIQFHWLLLLIHFVPRRWGKWFIDKRAYRFL
                     PKWSPSPLEALFTAVARDRLDIPRKRYYVRYWHFMKKRFSREHIFPKKDSVVSGAAGT
                     RR"
     gene            6018..6833
                     /locus_tag="P5079_02265"
     CDS             6018..6833
                     /locus_tag="P5079_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY28844.1"
                     /translation="MKTLLLTCDLEDFDVPADFGFSISEEKAREVTSEGLRRVLELAG
                     RLQGRMTFFVTASVVERFGEQIRELARMGHEIGVHAALPVSEGAFRAEEIQSLERARL
                     RLEREINARVYGFRSHKLRLPVDGLLGAAGYAYDSSLHPTYVPGRYCHLFKSRAIASS
                     GGVIEVPISVTPLLRLPFSWFWMRNGGIAYLKICSLWAWSQQDFLNVYFHNWDLADLE
                     KPVLRGRLPRFVRNNAGETMVKLLGKYLRWCGDRDIQCETIKEHLARKKLLSV"
     gene            7328..7765
                     /gene="nrdR"
                     /locus_tag="P5079_02270"
                     /pseudo
     CDS             7328..7765
                     /gene="nrdR"
                     /locus_tag="P5079_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            7897..10389
                     /locus_tag="P5079_02275"
     CDS             7897..10389
                     /locus_tag="P5079_02275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRY28845.1"
                     /translation="MPPISLTENQKKVIRDKYLRGDAGVEAWLNRVAGNIALAEVLFS
                     GQCDQWGVWNGVNCRITDVPTQAGKSTRLVLFHSGLHSARERGANFFKFLDNLKRVAV
                     EVPDARDRAETWRARFYQMMARFDFLPNSPTLMNAGGELQQLSACYVLPVEDSIEGIA
                     NAIKAQALIHKSGGGTGFSFQRLRPSGDEVKTTRGVASGAISFMQVFDKMTDVVKQGG
                     TRRGANMGILPYWHPEIEEFITLKNKAGVMENFNISVAVDDKFMQAVERGEEYDLLNP
                     RTQKPAGRANAKKIFDLMVETAWRTGDPGIIFIDRINSSPSNPTPQFGQIESTNPCGE
                     QPLLPNEPCNLGSVNLANFVKGEWTHGELDWDRLGETVALAVRFLDDVIEVNNYPLAE
                     IEVLAKSNRRIGLGVMGWAETLVKLGLPYDSDDALRLARETMAFINAKALEASEALAK
                     ERGAFPHWRESIYDPQGPHFRGRERFPRNCARTTIAPTGTIALAAGLQGSGIEPFFAI
                     SYVRYNAQALDRLKNGDKPAEHDVLYEVNPLFREEARRRNFFGLKEEELWKKIDANHR
                     SVRGIAEIPEDVQRLFSSSHDVPVPFHVKIQAAFQEHTDNAVSKTINMPNSATVQEVR
                     ESYAQAWRLGCKGITIYRDGSKSQQVLNLGAAGERKQGDPLPVARDTSRGVSSEYYEI
                     RTGNGSLHVHIDYDEHGPYRIFTNLPPLGTDLSGLTAIVGILLSKYLEAGGDPIRILK
                     HLNSVKGDRPFGFGAQRIDSIPHAIAVALRTHLQKTGKLEASEPAAAPKSLELWDHSA
                     ALYCPTCYSNNVSRESGCSGVTCHDCGYSECS"
     gene            10412..10969
                     /gene="dcd"
                     /locus_tag="P5079_02280"
     CDS             10412..10969
                     /gene="dcd"
                     /locus_tag="P5079_02280"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRY28846.1"
                     /translation="MSIKSDGWIRRMAREERMIEPFVESQVREGVISYGVSSYGYDIR
                     VGNHFKVFTNAYGSVVDPKRFDPKSFVDISTDTFVVVPPNSFALAQTIEYFRIPRNVI
                     TVCLGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPARIYANEGIAQVLFFEAD
                     EVCEVSYADKKGKYQKQTAITLPKI"
     gene            10966..12096
                     /locus_tag="P5079_02285"
     CDS             10966..12096
                     /locus_tag="P5079_02285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY28847.1"
                     /translation="MSRHSIELIEKGVNPLTDIADLEDRGVFVVETLAGDVAPTEQMQ
                     RFLERLQHEKKETFFGDVLYYLTSERYPESQAAVMWTEIMNHKFYMSEKLKRNIGIRV
                     AAIDYLVNIRGLLLAPRIMNSNDYRQTVKMARTDPLTGLYNRRYFIDQTNRFLEAANR
                     LKAPVTLMMTDLDHFKQFNDQNGHQAGDLILQEVARLVRGCVRNSDIVARYGGDEMAL
                     LLPKANRVDAVAVAEKIRVAIEENCHEMGITISIGIAQYPDDATSRDDLISAADDVLY
                     RAKEFGGNKAFYFHPISIRYDAKDPQVQTVSVVGDFNNWSKRTLPMARQPNGEWELTV
                     RLKPGRYRYKLFVNNGQWVPDPSAPLFEDDGFGGQCSIMVVR"
     gene            12127..13290
                     /locus_tag="P5079_02290"
     CDS             12127..13290
                     /locus_tag="P5079_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HRY28848.1"
                     /translation="MAFFIGRNREARRAYGFDEVAIVPGTVGVDPEDTDTGTAIGSLK
                     LKIPFIASAMDGTVDVKFAVAMGKLGGLAVLNLEGLHARYEKPEEAYAEIAKSTPDDA
                     PKVLQRIYARPIREDLIAQRIKEIKKGGAPAAVSCVPRHANRFGPLAAQAGADLFVVQ
                     GTVATARYSSSKGEPLDLAKLCRQLKIPVVIGNVVTRSAALELMETGCSGVLVGVGPG
                     AACTSRGVLGIGVPQITATIDCAASRDTYFKKTGRYVSIITDGGMTNSGDVCKAFAAG
                     ADAVMMGSTFARAKEAPGRGYHWGMAMPDRYLPRGTRIYVGAKGSLEEILFGPATQEE
                     GLQNFVGAVRTCMGYAGSRTIKEFQMAELIIAPEIKHEGKLFQQAQRVGMGKK"
     gene            13305..13457
                     /locus_tag="P5079_02295"
     CDS             13305..13457
                     /locus_tag="P5079_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28849.1"
                     /translation="MENDPELEELKRLLSPDQLNTDGASLADALVYVNYWRGFSAFDA
                     GGGPQN"
     gene            13650..15176
                     /gene="guaA"
                     /locus_tag="P5079_02300"
     CDS             13650..15176
                     /gene="guaA"
                     /locus_tag="P5079_02300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRY28850.1"
                     /translation="MILILDFGSQYTQLIARRVREAGVYCEIHPYNYPFGKIAALRPK
                     GVVLSGGPASVYANRSPRPDGRLFGLNVPTLGICYGLQLLVTHFGGHVVKSRKREYGP
                     AEIGVTQDNELFRGLPRTLNVWMSHGDAAEKLPEDFVPLAQTPSAPYVAIRQKNTNVF
                     GVQFHPEVHHTPQGKEIIANFLFRVCGEKPDWTMASFAETQVQSIRRQVGREKVILAL
                     SGGVDSSVAAALLHRAIGRQLHCIFVDNGLLRAGERERVRKIFGAAAGYETEIVDARK
                     RFLKKLKGVADPERKRKIIGREFISVFEERARRLKGVKYLAQGTLYPDVIESVSVKGP
                     SATIKTHHNVGGLPARMRMTLVEPLRFLFKDEVRALGRELGVPEEILLRQPFPGPGLA
                     VRILGEVTEEKLRKVREADLIVEQEIRAVGLYQKLWQAFAVLLPVNSVGVMGDERTYE
                     NAIVVRAVNSVDAMTADWARLPEEVLSKISSRIINEVRGVNRVAYDISSKPPATIEWE
                     "
     gene            15179..16063
                     /locus_tag="P5079_02305"
     CDS             15179..16063
                     /locus_tag="P5079_02305"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRY28851.1"
                     /translation="MNATTSLPFKLWRRGKVRDVYDLGDRLLIVATDRISAYDHILPT
                     PIPQKGVVLGRLSNFWFRRLEGVCPNHLIETNPALFPLELRRACAGMEGRAVLVQKAV
                     RIDIECVVRGYLAGSAWKEYRSGGVVCGVKLPPGLKESSPLPRPLFTPATKEEVGVHD
                     ENISFEKMASRVGKSVAEQLKELSLKIYGEAVRYAASRGFILADTKFEFGMHGGRLIL
                     IDEVLTPDSSRYWDAETYEEGKPQDSFDKQYVRDYLDRVGWKHLPPAPALPDEVVRQT
                     REKYVQAFERLTGEMFNG"
     gene            16056..16532
                     /locus_tag="P5079_02310"
     CDS             16056..16532
                     /locus_tag="P5079_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28852.1"
                     /translation="MAEETWRVVVYRRPGCPDHRGRSAEKDWRAARLGPLKRIRTGQA
                     YDLTGSVTAPTARALAGKLLVDAVMQEAEVRGVGAGGKSARGFCRAVIWPRQGVTDSV
                     GETVRLGARDLRLPVPDKVRAGALFDFWGAAPRKVRRFCETHLMNDLVHRLELEKC"
     gene            16526..18934
                     /gene="purL"
                     /locus_tag="P5079_02315"
     CDS             16526..18934
                     /gene="purL"
                     /locus_tag="P5079_02315"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRY28853.1"
                     /translation="MLRGPDFLEASPMELAALSRRRRLSLSDPELAAIRQHFKRLGRN
                     PTEAELETLAQTWSEHCKHKTFRGTIDHVERDELGMERRRRYGDLLKETIMRATDEIR
                     APWCVSVFRDNAGIVSFDGRRAVAFKVETHNHPSALEPYGGAGTGLGGVIRDVLGAGL
                     GARPIANTDVFCFAPLEQKDVPREGGALSARRVFHGVVSGVRDYGNRMGIPTVNGAVC
                     FDEGFSQNPLVFCGTAGVLPVSAVRKEVRPGDRIVMVGGRVGRDGIHGATFSSDNLSH
                     GIPASVVQIGNPIVQKKMMDALLQAREKGLYRAVTDCGAGGLSSAVGEMGEQSGAEVQ
                     LEDVPLKYEGLAPWEIWLSESQERMVLAVPPQNLEALKALFSAEDVLCVDIGFFRNDG
                     RLRVLYGQETIVDLPADFLHNGAPRRHLSSSWSPLRIPAEWPRKGGRARREEDVRETL
                     LEILSSPTVASKEWIVRQYDHEVQGRTVLKPLVGPAGRGPGDAAVMRLDWETHAGVAL
                     ANGLNPRYSRWDPYWMAASAVDEAVRNLIAVGASRDHVALLDNFCGGNPNEPKVLGEL
                     VRAAQACYDIARAYGTPFISGKDSLFNRFVDKKGARKDIPTTLLISALAWMDDVRRAV
                     TMDAKKAGDLIFIVGLTRDELGGSHYGLVKNIVAGRVPRLYPEETAPLYDALSEAMER
                     GWVTACHDCSEGGLGVALAEMALAGELGIYADLRRAPLDGAVWDDSRTDKALFSESNG
                     RFVVEVPPRFRRYFLSRFEGMPAACVGEVREGRKLQTVGLEGKSRAWALSDLAAAWTG
                     GVRP"
     gene            18931..19716
                     /gene="purQ"
                     /locus_tag="P5079_02320"
     CDS             18931..19716
                     /gene="purQ"
                     /locus_tag="P5079_02320"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HRY28854.1"
                     /translation="MTVRAVVLRCAGTNCDEETAAALRRAGAEPSRVHVNRFLEGGRR
                     LNDYDLLVIPGGFSYGDDIASGKVLANQLSHRLKDQLDEFIARRKPVIGICNGFQALL
                     KGGFLPGRAGVSVSATLTDNDSGKFECRWTFLRTSSGKCPFVRGLPAVFPAPVAHGEG
                     KFVPESEAGLRTLEKQGQIVFRYVNERGQSDGYPWNPNGSRGDVAGICNREGNVLGLM
                     PHPERYFYPQQHSHWTRIRPLPEHGVGLQIFKNAVRYAAQVAS"
     gene            19728..21140
                     /gene="purF"
                     /locus_tag="P5079_02325"
     CDS             19728..21140
                     /gene="purF"
                     /locus_tag="P5079_02325"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRY28855.1"
                     /translation="MKIGPRPSFALPFDRMPEECGVFGIYNHAEASRLAYLGIHQLQH
                     RGQESAGIVTTDGKRFYTHVAMGLVADVFNRPLLDDMKGRTAIGHVRYGTSGVSSLVN
                     AQPIVVTTSRGPMALAHNGNLTNALLLRRQLEKEGSIFQTSSDSEVILHLVAKSSARD
                     LGAALKEAFSQVEGAYSIVLLTPKTLFVARDPWGVRPLHIGRLNGSFVVASETCAFDI
                     INARLVREVKPGEIVAIDGQGIRPIAQLSSTRLAHCIFEFVYFSRPDSRIFGRSVYEV
                     RRELGRQLARESPVKADVVVAVPDSSSCAAVGYAERSGLPLELGLIRSHYVGRTFIEP
                     KQAIRDFGARMKYSAVADAVRGKRIVLVDDSIVRGTTSRKLVRMLRRVGVKEIHLRIS
                     SPPILGPCFYGIDTPETKELIAARLSVEKIREYLRADSLKYLSLGGMLKATKMNPGDF
                     CTACFTARYPIPLRTNGTIK"
     gene            21181..21699
                     /gene="purE"
                     /locus_tag="P5079_02330"
     CDS             21181..21699
                     /gene="purE"
                     /locus_tag="P5079_02330"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRY28856.1"
                     /translation="MKRSGKKSPQAAVVMGSESDLPVMRQAAESLEQFGIPFEMHVLS
                     AHRTPHETVRFAAGARRRGIRVIIAGAGMSAHLPGVIAAITELPVIGVPVSGGPLQGQ
                     DSLFSIVQMPRGVPVATVAIGNAWNAGILAAQILGAGGSGALLDRVARYKKQLKKKVL
                     SRKVGLDAPREK"
     gene            21704..22975
                     /gene="purD"
                     /locus_tag="P5079_02335"
     CDS             21704..22975
                     /gene="purD"
                     /locus_tag="P5079_02335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRY28857.1"
                     /translation="MNVLIVGGGGREHALAWSLKQSARVDRLWCSPGNAGIAAHADCL
                     PLDFDDKQKVAAFHRQNKIDLTVVGPELPLVKGLADTVRSFGGLVFGPGRLAARLEGS
                     KTFAKSFMKTHGIPTADFRIFSEFEEASAFIRSGEWPESFRVVKADGLAAGKGVRVCA
                     DRNEVLEAVEEMMVRRAFGAAGENVVLEEMLAGEELSVMAFCDGKKLLPLLPCRDYKR
                     VGDGNVGPNTGGMGAYSPVSAISEALWARIEKTVFQPFLRGIRSEWLDYCGAIYFGLM
                     LTPEGPRVLEFNVRFGDPETQAVLPLIGNDLFELISATALGELGDVALRRRAQASVCV
                     VLASKGYPGDFETGKPISGLEPWADAPDVAVFHAGTRRRDSQIETAGGRVLGVTAWGP
                     DLAKARARAYEAADKIKFENVCFRRDIASEG"
     gene            22979..23551
                     /gene="pyrR"
                     /locus_tag="P5079_02340"
     CDS             22979..23551
                     /gene="pyrR"
                     /locus_tag="P5079_02340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRY28858.1"
                     /translation="MKKKIRTAAQVKSLFDRLAADIRKTVKEPRADWVFVGIQRRGVP
                     LARRLSALLAAPGGPAAVGTLDITFYRDDSQAVPLHPVVHDTELPFDVTGKTVFIVDD
                     VLFTGRTVRCALDELMDFGRPRRVYLGVLIDRGHRELPIQADFVGEALRTDCDETIDV
                     HLRETDGEDAVWRSGRPEKPASKRKTEVAR"
BASE COUNT         4545 a         6495 c         7681 g         4891 t
ORIGIN      
        1 cggactccat cgatctggcc gaggaccggg agcggttcgg cgctttgctg aagaggctca
       61 aaatcccgca cccggcccac ggtgccgccc ggacgttggc cgaagcgcag cgggcggcgg
      121 cccgggtggg gtaccccctg atggtgcggc cgtcctacgt gttgggaggc cgggccatgg
      181 aagtggtcta cgacgacaaa cagttggcgg gttacatgga ccgggctctc gcgggggggg
      241 cggggctgcc cattctgctg gaccggtttt tggaggcggc caaggaagtg gacgtggacg
      301 ctatttccga cgggaaagat gttttcatcg gcggcatcat ggaacacatc gaggaggccg
      361 gcatccattc gggggactcg gcctgcgttt tgccgcctta cagcctggac gaaaaaatcg
      421 tagagacgat cactgcccag acgcgcaaac tcgccaaggc gttaaaggtc aaaggtctgc
      481 tcaacatgca atacgccatc cgggacggcg tggtttatgt tctcgaagcc aacccgcgcg
      541 ccagccgcac ggtgccgttc gtctcgaagg ccacgggggt gcctctggcc aagatcgccg
      601 ccaaggtgat ggccggcttg ccgctgaaaa agtgtctggc cccctggcga aaggtgttgg
      661 cccggcgaaa atcctggtac gcggtgaaag aggtggtttt cccgtggatg cggtttcccg
      721 aggtggacgt ggttttgggg ccggagatgc ggtccaccgg cgaggtgatg gggatcgacg
      781 gcggggtggg gttggcgttc gccaagtcgc aggcggccgc gggcggggcg ttgccgaaga
      841 gcgggaaaat ccttttcagc gtgagggaaa aagaccggcc cgaggcgttc cgcgtggcga
      901 gcggtctggc cgcgttgggg tatcgactgg ccggaacctc cggcacggcc gcctatctgc
      961 gttctcgggg attgacgatc gaatccgtga agaaattgtc cgaagggcat ccgaatgtcg
     1021 tggatgtgat tcgtgacggg gaggtgtcgc tcctcatcaa cacgccggac ggtccccggg
     1081 cccggtcgga cgggttccat atccggcgga ccgcgctcct gcacaacgtg ccgctgataa
     1141 ccaccctggc cgccgcccat gccgccgtgg aagggcttca gcaatctttt gtcagtccgt
     1201 ggcggataca ttcgcttcag gaattgcatg cgttgccggc ggacaatcgg accattgacg
     1261 gataggggtt tcggatggac atccttctcg cgccatgacc agccctaaag gtttcttggg
     1321 cttttcgggg ccggtcaacg cctggcggaa ttcgtttcat gtggttgtcc tggtcggcat
     1381 tgtcgtcctt tatgcggtgg tcaactactt ctggcttcaa aaaacgtgtt tcgtcccgca
     1441 taatccagat gaagcgtttc atatctatac cggtgttttc aatcggtatt atcccgttga
     1501 aaatatcggt cagcttgttt cggaaatacg aaaggcggta cgggagccgc tttacggctt
     1561 aatggccgtc gttgcgaata aatatttttc aaagagcctc tttgtaaccc gatacgtcaa
     1621 tttgttttgg tttgccatcg ccttgttcgc cgtgtatcgg ctcgggacgg ttctttatga
     1681 ttccagggcc gggttataca gcgccttcgt gatggcgttt tatccggcga tgttcggttt
     1741 ttcgaggatc tattcccccg agttcgggct gataggggtc atgactttcg ctgttgtatt
     1801 tttgtttcaa agcgatggat ttttcgtcac acggtctgcc gttctttttg gtttgatgtt
     1861 gggtatgggg ctgatgataa agaacataat ggcccttttt attgtaggac cgctggtcta
     1921 cgcggtgttc cggcatcggt ccttcaaata tgtccgtgtc cggaacatca tcgtctcttt
     1981 ggggattgca ttgatcctta taatttggag atacagcaat gttgaattgc tcagcctgta
     2041 tctgcagtct ccattcgggg agagttccgg ggcatggcat gagtggagga acctgcgtgt
     2101 tctgacttgg gatgccgtgc gctatcagtt ttcgcccatt ttttttggcc tattcttttg
     2161 gagcttgagt ggatttttga aacagggtcg gaaagatgga agagcgatgt tgttgctgtg
     2221 gatcgttatt ccggtggtgt ttttcattct aataccgcat cggaaggtcc ctcgctatgt
     2281 tatcccctat ctgcccgcct tcgccgtcat ttccgggata gggctcgccc ttctggaacg
     2341 ccggcggcag cgggtgattg tgagcgtttt tgttgttttg gggctggttc aatttttttt
     2401 atgttcattt ccgggaaggc gggattttaa ggaaagcggt ttattttttt gcggccggga
     2461 ggattggaag gatccggccc tctgcgtcga gcggccgtgc ggcgaaaagt gttttcgccc
     2521 catcgcggaa gttatcgaag ggacttcgga gaaaaaaaat ccgttcctcg tggtttttcc
     2581 ggaccgaaga cattttacgg ttaaatgggc gttgttcagg tggtttaatg atgtccagct
     2641 ccggttggcc cttctggaaa gggagaatct attccaaatg gagccttacg ttaaacaagc
     2701 ggactatctt ttgatgcccg cgatggcttg gggggcaata ggagacgatt atttcgacca
     2761 acagatatcc ctcgcgaaac agcactttga atggccgacg aaaaagaatc aaaaacttgt
     2821 cgattttgtt aacggctatt ccgttgcata taaaaaacgg ttgattgatg aaatggggga
     2881 atttcaggaa atcggacgct ttgagctttc cttggaggac gacactttcg tgttgttgaa
     2941 aagaaaaaaa gagacgttgt agggaaggca ccgtgatttt tgggggagtc cattgcggta
     3001 ataatcaggt gtgagggggg catccgcctg agagactcgg agttcggagg gtgaaggcat
     3061 gcgaatcgtt tttgtttttc cgccttttga ccacaagaaa tttgaagaag acatcgatgt
     3121 cgtaagtcgt gaatttaccc agcctcctcc gctcggattg gcctacgccg ccgccatcgc
     3181 tgaaaagtcg ggccacgacg caatcattat cgatgccaac cttcgtccgc ggatgtccaa
     3241 agaagacgtc ctcgcgcaga taaacgaatt caaaccacag attctgtcgt ttatgttgac
     3301 ggcctatatg ttccaacaga cgttggagtg gatcgtattt ttgaaacaga aaacgggatt
     3361 gcctgtcttg gtggggaata ttttgatgga gttgtatccg aaggaggttt tttcgcatcg
     3421 tgaaatcgat tttggaatta taggttcggc acaaaagcca ttgccggcgt tgctgcgtgc
     3481 gcttgaaaat ggcggttccc tggaggggat caaaggtctt tgttacaggg agaacgggca
     3541 gcttgtcatt cagccgccgg atacccttcg ggaggatttt gattcgctcc cgtttccggc
     3601 gcggcacttg ctggccaacg atcggtatta tacggtcgtt tcgaaaagaa agaatttcac
     3661 catcatgatg acctccaaag gatgcgtcag ccggtgcggg ttttgtcatg tgaaggacat
     3721 cccctattcg gcccggtcgc cggtcggcgt cgctgacgag ctggaggaat gttacgggcg
     3781 gcatgggata cgggagattg aattctttga tccggtgttt accgtgaaca gggcccgcgt
     3841 ccgggagatt tgtcttgaga tccgccgtcg agggctggat atttcgtggg cttgtcgcgc
     3901 tcgggtggac caggtcgacg aggaattgtt gcgggagatg aaggcggccg ggtgcgctcg
     3961 gatttattac ggcctggaat gcgggaacca ggcgattctc gacaaaacga gcaaaggcat
     4021 cacgctggaa cggattcgcg aggtggttcg aataacacaa aattgcggca tattgaccct
     4081 cgggttcttt ttgattggcg ctccgggaga gacggttcgg acgatagaag agacgattcg
     4141 tttcgccatg tcgctgggat tggattatgc tcaattccat aaaaccgttg cgaagccgaa
     4201 gacgctcctc tacgatgagg ttatgcggac gacggggcgg gactactggg gtgaattcgt
     4261 catggggacg gcaaaggagg agcgattgcc gactccgtgg acggtggttt cggacggcga
     4321 catagaaaaa ctgaccgtgc gcgcgtatag gaggttctat tttcagccga gccgcttgtg
     4381 gaagatattt cgagggatca agtccttttc cgaatttggg agatatgtca ggtccgccgt
     4441 cggtatttgg cgtgtaaaaa gcgatttgca tggaagacgg atagcgccat gagggtgatg
     4501 ttcgtttatg gggcgttcga aaatttgggg atagaatacc tatcggctgt attgaaatcg
     4561 catcaccata cgacgcgctt ggcgctggac cccctgcttt ttgacgaccc tttccttcga
     4621 ttgccgccct tggcgcggtt ttttgaccat caaaaaagtt tgattcggga gatcgccgaa
     4681 ttcgggccgg ggctcctggc cttctctgtc cccagctgtt actataaaaa ggccctgtct
     4741 ctggcaaatg ccgttaagcg gatccttccc tcggtgcacg tcacgtttgg cgggatccat
     4801 cccaccgccg ttccggagac ggtgctggaa aacgactgcg tggattccgt tgtgttgggg
     4861 gagggagaat atccgttgtt ggagctggcg gattgtttgg ggcgaggcag gttggatacg
     4921 gccatccgta atatttggtt tcgccaggcc ggggggatcg tcaaaaatcc cgtgaggccg
     4981 tatatctcga atctggacga gttgccttat cccgacaagg gtctctatta cgatatactt
     5041 ccgcggtatc ggaaaggcta tacggtcatc acgcgacggg gttgcacggg gggatgttcc
     5101 tattgttgcc tcaatgtgtg gcacgcatta tatccggggc agccgcggat ccgaatccgc
     5161 ggcattgaaa acgtgatccg tgagctccgg gaggcgaaag aacgttataa tttcacgcag
     5221 ctccgaataa acgacgattt gttcacgtac gacaaaaatt ggctgcgtga ctttgcgaaa
     5281 gaatacaaac ggcatatcgg gacgccggtc tggtgtttca cttctccggc gtatgtggac
     5341 gaggaggtgg tccggagtct tgagaagatg tcgtgtcgtc aagtatgttt ggggatccaa
     5401 agccccgtcc gggcccttcg agaaaggatc ctccatcgct atgtggacaa cgagaagaac
     5461 acgcgcgcca ttcaattatt gaaggcggcc gggattcgct gtttggtgga tacgatcatg
     5521 ggatttcccg gagagacgga agacgatttg gtggaaaccg cgcggtatta taacgaaacc
     5581 cggcccgata gaatctgtat tttttggctc atttattatc cccggacgcc cctcgtggaa
     5641 atggccaagg gaatgggtgt cctcgatgaa acgcagtgcg cacgtttgga atgcgagccg
     5701 acgcacctgg ccaatacgat cccggatcgg cgtcgttatc tgcggaagat acaattccat
     5761 tggttgttgc tgttgattca tttcgtccct cgccggtggg ggaagtggtt tatcgataaa
     5821 agagcgtatc ggttccttcc caaatggagt ccttctcccc tggaggcgtt gttcaccgcg
     5881 gttgcgcgag accggttgga tatcccgcgg aagagatact atgtccgtta ttggcatttc
     5941 atgaaaaaaa gattctcccg ggaacatatt tttccgaaga aggattccgt tgtttccggg
     6001 gcggcgggga cgcgccgatg aaaacgttgc ttttgacttg tgatctggag gattttgacg
     6061 tgccggccga tttcgggttc agcatttccg aagagaaggc aagggaggtc acgtcggaag
     6121 ggcttcgccg agttctggag cttgccggaa gattgcaggg gaggatgaca ttttttgtga
     6181 cggcgtccgt cgttgaacgg tttggtgaac aaataagaga attggcgcgg atggggcacg
     6241 agatcggagt tcatgcggcg ttgccggtgt cggaaggggc gtttcgagca gaggaaatcc
     6301 aatcgctgga acgggcccgg ctgcgcctgg aaagagaaat aaacgctcgc gtgtatgggt
     6361 ttcgaagcca taaactgagg ttgcccgtcg acggacttct cggcgctgcg gggtatgcct
     6421 acgattcgtc gcttcacccg acctatgtgc cgggtcgata ttgtcacttg ttcaaatccc
     6481 gcgccatcgc ttcttccggg ggggtgatcg aagttccaat ttcggtcaca ccgttgttga
     6541 ggcttccgtt ttcttggttt tggatgcgca acggcggaat cgcctatctg aaaatttgta
     6601 gtttatgggc gtggtcgcaa caggattttt tgaacgtcta ttttcataat tgggacttgg
     6661 cggatttgga aaaaccggtc cttcggggtc gactgccgcg cttcgttcga aacaatgccg
     6721 gcgaaacgat ggtcaaactt ctgggaaaat acctgagatg gtgtggcgat agggacatac
     6781 agtgtgagac cataaaagag catttggcgc ggaaaaaact gttgtctgtg tgagagaaag
     6841 aagaaacggg gacggagaag ctttttatcg atacgtggag gtttaaaagg agcgactggg
     6901 cgggggagtc ggaggtttta ttgcaatgtt gtaggaaggg atcccaggcc catccctggc
     6961 ggatacgggc cctccaggtt ctgcacagtt tgacgaattc gccgtggtaa ggtttttttg
     7021 ctcagtcggg agttatcccc aaatgtgaaa agttgttcac agcccgatca ggcgcttgaa
     7081 taggagcaaa ataacgcatt tattttcatt tttgaccgct ttcggccccc ccttctcttc
     7141 ctatataaag gattttcgct gacccccctc cgggggtttc cggctccaag agacctcaaa
     7201 aaaatcccac ttgacatact acaggttgtt gttctaaaat aatccacaca tgatattgat
     7261 tttcgatttc gccgttcctc cgtcaaccga tagggccttg tgagccgcct cgcccctagg
     7321 gattgttatg cgatgccccg cttgtgacca tccggaaggc cgcgtgatcg actcgcggcc
     7381 gctcgattcc gccacggtga tccgccgtcg gcgcgtctgc gggcattgcg gaaaacgcta
     7441 caccacctac gaacgcgtgg agtccacccc gctcatggtc atcaagagcg acaaccgccg
     7501 cgagccgttc gaccgcaaga aagtccggga gggcgtgatc cgggcctgcg agaagcggcc
     7561 gatcgcttcc gacgtcatcg agaagctggt ggcggaggtg gaatacgaac tgcaggacta
     7621 tgtgatggag gtccccacgc gggagatcgg cgagcggatc ctcaagaaac tccacgacct
     7681 ggatgcggtg gcctatgtgc ggttcgcgtc ggtctaccgg cagttccgcg atctggacga
     7741 ttttctcaag gagttaaaaa aactgaagag gaaacacatg cgggaacgcg cccacaagca
     7801 gcagggggaa cacgtcgaga agtcccagcg ggaactcgtg cacgcggggc cactccactg
     7861 agccggccga gcgtgccgct tcaggagccg gccgaaatgc ctccgatctc tctcaccgaa
     7921 aaccagaaga aagtcatccg ggacaaatat ctccggggcg atgccggcgt cgaggcgtgg
     7981 ctgaaccgcg tggcgggaaa catcgccctc gccgaggtcc tcttcagcgg ccagtgcgac
     8041 cagtggggcg tctggaacgg cgtcaattgc cgcatcacgg atgtccccac gcaggccggc
     8101 aaatccacgc ggctggtgct gttccactcc ggactgcatt cggcgcggga acggggtgcg
     8161 aacttcttca aattcttgga caacctgaaa cgcgtcgccg tcgaggtgcc cgacgcccgc
     8221 gaccgggcgg agacctggcg ggcgcgcttt tatcagatga tggcgcggtt cgatttcctg
     8281 cccaactcgc ccacgctcat gaacgccggc ggcgagcttc agcagttgtc cgcctgttat
     8341 gtgctgccgg tggaagattc catcgagggc atcgccaacg ccatcaaggc ccaggcgctc
     8401 atccataagt ccggcggagg aacggggttt tccttccagc ggcttcgccc gtccggcgac
     8461 gaggtgaaga ccacccgcgg ggtggcttcc ggcgccatct ccttcatgca ggttttcgac
     8521 aagatgaccg acgtggtgaa gcagggcggc acccgccgcg gcgccaacat gggcatcctc
     8581 ccctattggc atccggagat cgaggagttc atcaccctca agaacaaggc cggcgtgatg
     8641 gagaacttca acatctccgt ggcggtggac gataagttca tgcaggcggt ggagcggggg
     8701 gaggagtatg atctgctcaa cccccgtacc cagaaacccg ccggccgcgc caacgctaaa
     8761 aagattttcg acctcatggt ggagaccgcc tggcgcaccg gcgatcccgg catcattttc
     8821 atcgaccgca tcaactcttc gccgtccaac cccacgcccc agttcggtca gatcgagagc
     8881 accaacccct gcggcgaaca gcccctgttg cccaacgagc cgtgcaacct gggttccgtc
     8941 aacctggcga attttgtcaa gggggagtgg acgcacgggg agctggattg ggaccgtctg
     9001 ggggagaccg tggcgctggc ggtgcggttc cttgacgacg tcatcgaggt gaacaactat
     9061 cccctggccg agatcgaggt gttggccaag agcaaccgcc gcatcgggct gggcgtgatg
     9121 gggtgggccg agacgttggt gaaactcggg ctcccttatg attcggacga cgccctccgg
     9181 ctggcccgcg agaccatggc gtttatcaac gccaaggcgc tggaggcttc cgaggctctg
     9241 gcgaaggaac gcggcgcgtt tccgcattgg cgcgaaagca tctacgatcc gcaggggccg
     9301 catttccgcg gccgggaaag gttcccccgc aactgcgccc ggaccaccat cgccccgacg
     9361 ggcaccatcg cgttggcggc ggggctccag gggtccggca tcgagccgtt cttcgccatc
     9421 tcctacgtgc ggtacaacgc ccaggcgctg gaccgcctca agaacggcga caagccggcg
     9481 gaacacgacg tcctctacga ggtgaacccg ctcttccggg aagaggcccg ccgacggaac
     9541 tttttcggtt tgaaggaaga ggagctctgg aaaaagatcg acgccaacca ccggtcggtc
     9601 cgcgggatcg ccgagatccc cgaggacgtc cagcgccttt tttcctcctc ccacgatgtg
     9661 ccggttccgt tccacgtcaa gatccaggcg gcttttcagg aacacaccga caacgcggtg
     9721 tcgaaaacca tcaacatgcc gaactccgcc acggtccagg aggtgcgtga gtcctacgcg
     9781 caggcctggc ggctcggatg caagggtatc accatctacc gcgacggctc caagtcgcag
     9841 caggtgctca acctcggggc cgccggggag cggaaacagg gggaccccct gcccgtggcg
     9901 cgcgacacct cgcgcggggt atcgtcggaa tattatgaga tccgcacggg gaacggctcc
     9961 ctgcacgtgc acatcgatta cgacgaacac gggccgtatc gcattttcac caacctgccg
    10021 cccctgggca cggacctttc cggcctcacc gccatcgtcg gtatcttgtt gtcgaaatat
    10081 ctggaggccg gcggcgaccc catccgcatc ctgaaacatc tgaattccgt caagggagac
    10141 cggcctttcg ggttcggggc gcagcggatc gattccattc cgcacgccat cgccgtggcc
    10201 ttgcgcacgc atctgcaaaa gaccggcaag ctggaggctt ccgaaccggc cgccgcgccc
    10261 aagtcgctgg agctgtggga ccattcggcc gcgctctatt gtcccacctg ctattccaac
    10321 aacgtttccc gcgagagcgg ctgttccggc gtcacctgcc acgactgcgg gtattcggaa
    10381 tgttcctgac gacaagaagg agaggttgat catgtccatc aagtccgacg gttggatccg
    10441 ccgcatggcc cgggaagaga ggatgattga gcctttcgtc gaatcccagg tgcgggaagg
    10501 ggtgatctct tacggggtct ccagttacgg ctacgacatc cgggtgggca accatttcaa
    10561 ggttttcacc aacgcctacg gttcggtggt ggatccgaaa cggttcgatc cgaaatcgtt
    10621 cgtggacatc agcacggaca ccttcgtggt cgtcccgccg aattcgttcg ccttggcgca
    10681 gaccatcgaa tatttccgca tcccgaggaa cgtcatcacc gtgtgcctcg gcaaatcgac
    10741 ctacgcgcgt tgcggcatca tcgtcaacgt gacgccgttt gagcccgaat gggagggttt
    10801 cgtgacattg gaaatctcga atacgacgcc cctgccggcg cgcatctacg ccaacgaggg
    10861 gatcgcgcag gtgctgttct ttgaagcgga cgaggtctgc gaggtgtcct atgcggacaa
    10921 aaaaggcaaa taccagaaac agaccgccat caccctgccg aaaatatgag ccgacattcc
    10981 atcgaattga ttgaaaaggg cgtcaacccg ctgacggaca tcgccgacct cgaggaccgg
    11041 ggggtcttcg tggtggaaac attggcgggc gacgtggccc ccaccgaaca gatgcagcgg
    11101 tttctggagc ggctccagca cgaaaaaaag gagacgttct tcggcgacgt tctctattac
    11161 ctcacctcgg aacgctaccc cgagagccag gccgccgtca tgtggacgga gatcatgaac
    11221 cacaagttct acatgagcga gaaactgaaa cggaacatcg gcatccgcgt ggcggccatc
    11281 gattatctgg tgaacatccg cggtctcctc ctggcgcccc gcatcatgaa ctccaatgat
    11341 taccgccaga cggtcaagat ggcccgcacc gaccctctca ccggcctcta taaccggcgg
    11401 tatttcatcg accagaccaa ccgtttcctg gaagcggcca accggctcaa agcgcccgtg
    11461 accctcatga tgacggatct ggatcacttc aaacagttca acgaccagaa cggccatcag
    11521 gcgggggatc tcatcctgca ggaggtggcc cgcctcgtcc gcggctgcgt gcgcaattcg
    11581 gacattgtgg cccgctacgg cggggacgag atggcgctcc tgctgcccaa ggccaaccgc
    11641 gtggacgcgg tggccgtggc cgagaagatc cgcgtcgcca tcgaggagaa ctgccatgag
    11701 atgggcatca ccatcagcat cggcatcgcc cagtatcccg acgacgccac ctcccgcgac
    11761 gacctcatct ccgccgccga cgacgtgctg taccgcgcca aggagttcgg ggggaacaag
    11821 gccttttatt tccacccgat ctccatccgc tacgacgcca aggacccgca ggttcagacc
    11881 gtcagcgtgg tgggcgattt taacaactgg agcaaacgca cgcttcccat ggcgcgccag
    11941 cccaacggcg agtgggagct gaccgttcgg ctcaaacccg gccggtatcg gtacaaactt
    12001 ttcgtcaaca acggccaatg ggtgcccgat ccgtcggcgc cgcttttcga ggacgacggg
    12061 ttcggcggcc aatgttccat tatggtcgtc cgctgacgca gcgctctcac ggggaggagc
    12121 ttcgacatgg cgttttttat aggacggaac agggaggccc ggcgggccta tgggttcgat
    12181 gaagtggcca tcgtccccgg cacggtcggg gtggatccgg aggacacgga caccgggacg
    12241 gccatcggct ccctgaaact gaagatccct ttcatcgcca gcgccatgga cggcacggtg
    12301 gacgtcaagt tcgccgtcgc catggggaaa ctgggcgggt tggcggtgct gaacctggaa
    12361 ggtctccatg cccgctacga aaaacccgag gaggcctacg ccgagatagc caaatccacc
    12421 ccggacgatg cgccgaaagt cctgcagagg atctatgccc ggcccatccg ggaggacttg
    12481 atcgcccaac gcatcaagga gatcaagaag gggggcgctc cggcggcggt gtcctgcgtg
    12541 ccgcggcatg cgaaccgttt tggtccgctg gcggcccagg ccggggccga tctgttcgtc
    12601 gttcagggca cggtcgccac cgcccgctat tcctcgtcca aaggcgaacc gctggacctg
    12661 gccaaactct gccgccaact gaagattccg gtggtcatcg gcaacgtggt gacgcggtcg
    12721 gccgcgctgg aattgatgga gaccgggtgt tccggcgtcc tcgtcggcgt ggggcccggc
    12781 gcggcctgta cctcccgcgg cgtgttgggc atcggcgtgc cgcagatcac cgccaccatc
    12841 gattgcgccg cttcgcggga cacttacttc aaaaagaccg gccgatacgt ctccattatc
    12901 accgacggcg gcatgaccaa ttccggggat gtctgcaagg ccttcgccgc cggggccgac
    12961 gccgtgatga tgggttccac cttcgcccgg gccaaagagg cgcccggccg gggctatcac
    13021 tggggcatgg ctatgcccga ccgctatctt cctcgcggca cgcgcatcta tgtgggcgcc
    13081 aaggggtccc tggaggagat cctgttcggg cccgccacgc aggaggaggg actccagaat
    13141 ttcgtcggcg ccgtccgcac ctgcatggga tacgccggtt cgcggaccat caaggaattc
    13201 cagatggcgg agctcatcat cgcgccggag attaagcacg aagggaaact ttttcagcag
    13261 gcccagcgtg tgggcatggg taaaaaatag ggtttggagg acgtttggaa aacgatccgg
    13321 aactggaaga attgaagcgg ttgttgtctc ccgatcagtt gaacacggac ggcgcctcat
    13381 tggccgatgc gttggtctat gtcaactatt ggcgaggttt ttccgccttt gacgccggcg
    13441 gcgggcctca gaactgaaga ggtagtgctt ccgtcccttc gggcggtttc cggagggacg
    13501 gaaggaatga agtatttctc gttcgacggt tttcctctcc caacggaaaa cccgcggtgt
    13561 tctcccggtc attcttcgac gacatttcta tttcgctgta aaaatcgact tcccttcgac
    13621 atcccgcgga gaggttgaaa aagaagatca tgatactcat ccttgatttt ggttcgcagt
    13681 acacacagct catcgcccgg cgggtgcgcg aggccggcgt gtattgcgag atccacccct
    13741 acaattatcc cttcggaaaa atcgcggcgt tgaggccgaa aggcgtcgtc ctttccggcg
    13801 gcccggccag cgtctatgcg aatcggtctc cgcgtccgga cgggcgtttg tttgggttga
    13861 acgttccgac gctcggaatt tgttacggcc tgcagttgct ggtgactcat ttcggcggac
    13921 acgtggtgaa atcccggaaa cgggaatacg gtccggccga aatcggcgtc acgcaggaca
    13981 atgagctttt tcggggcctt ccgcgcacct taaacgtttg gatgagccat ggcgacgcgg
    14041 cggaaaagtt gccggaggat ttcgtcccgc ttgcgcaaac gccgtccgct ccctatgtcg
    14101 ccatccgtca aaaaaacacg aatgtgttcg gcgtccagtt ccatccggaa gtgcatcaca
    14161 cgccgcaggg aaaagaaatc atcgccaatt ttctgttccg tgtttgcggc gaaaaaccgg
    14221 attggaccat ggcttccttc gccgaaacgc aggtgcagtc gatccgccgg caggtcggcc
    14281 gcgaaaaggt gatcctggcg ctttccggag gggtggattc ttccgtggcc gcggcgcttc
    14341 ttcaccgcgc catcgggcgg cagctccatt gcatattcgt ggacaatggg ctgttgcggg
    14401 ccggcgaaag ggagagggtg cggaagattt tcggcgcggc cgccggctac gagaccgaga
    14461 tcgtggacgc gcggaaacgg tttttgaaaa aactcaaagg agtggccgac ccggaacgta
    14521 aacgcaagat catcggacgc gagtttatct cggttttcga ggagcgcgcc cgccgactca
    14581 aaggcgtcaa atatctcgcg cagggcaccc tttacccgga cgtgatcgag agcgtttcgg
    14641 tgaaggggcc ttccgccacc atcaagaccc atcataacgt cggcggcctt ccggcgcgga
    14701 tgaggatgac cctggtggag cccctgagat tcctttttaa ggacgaggtc cgtgcgttgg
    14761 gccgggaatt gggcgtgcct gaggaaattt tgttgcgcca gccgttcccc ggccccggcc
    14821 tggcggtgcg cattttgggc gaggtgacgg aggagaagtt gaggaaagtg cgcgaggcgg
    14881 acctgatcgt ggaacaggaa atccgggccg tggggctgta tcagaagctc tggcaggcct
    14941 tcgcggtgct cctgccggtg aacagcgtgg gggtgatggg ggacgagcgg acctatgaga
    15001 acgccatcgt ggtgcgcgcc gtgaattccg tggacgccat gacggccgac tgggcgcgct
    15061 tgcccgaaga ggtgttgtcg aaaatttcgt cccgcatcat caacgaggtg cgcggcgtga
    15121 accgcgtggc ctacgacatc tcgtccaaac caccggccac catcgaatgg gaatgaacat
    15181 gaacgcgacc acgtctttgc cgtttaaatt gtggaggagg gggaaggtcc gcgatgtcta
    15241 cgatctgggg gacaggctcc tcatcgtggc cacggaccgc atctccgcct acgaccacat
    15301 cctgccgacg cccatcccgc agaaaggcgt ggtgctcgga cggctctcca atttttggtt
    15361 ccgccggctg gaaggcgtct gcccgaacca tctgatagag acgaacccgg ccctttttcc
    15421 gctggaactc cgccgtgcct gcgccggcat ggaaggccgc gccgtgctgg ttcaaaaagc
    15481 cgtgcgcatc gacatcgaat gcgtggtgcg cggctatctg gccgggtccg cctggaagga
    15541 ataccggtcc ggcggtgtcg tctgcggcgt taaactgccg ccgggactga aggagtcttc
    15601 cccgctcccc cggcccctct tcacgccggc caccaaagag gaggtgggcg tgcacgatga
    15661 gaacatctcg ttcgagaaga tggcgtcccg ggtggggaaa tccgtcgccg aacaactgaa
    15721 ggagttgagc ctgaagattt acggggaggc cgttcgttac gccgcttccc ggggcttcat
    15781 tttagccgac accaaattcg agttcgggat gcacggcggc cggctcatcc tcatcgatga
    15841 ggtgctcacg ccggactcgt cccgctattg ggacgccgaa acctacgagg aggggaagcc
    15901 gcaggactcc ttcgataaac aatacgtccg cgattatctg gaccgcgtcg gttggaagca
    15961 cctgcccccg gccccggcgt tgccggacga ggtggtaagg caaacgaggg agaaatacgt
    16021 ccaggctttt gagagactga ccggggaaat gttcaatggc tgaggaaacc tggagggtcg
    16081 tcgtttatcg ccggccgggc tgtcccgacc atcggggccg gtccgccgaa aaagactggc
    16141 gggccgcccg gttgggtccg ttgaaaagga tccgcaccgg acaggcttac gacctaacag
    16201 ggtcggtaac ggcgccgaca gcgcgggcgc tggcgggaaa attgctggtg gatgccgtca
    16261 tgcaggaggc ggaggtgcgg ggcgtcgggg ccggcgggaa gtccgcccgt ggtttttgcc
    16321 gcgccgtcat ctggccgcgt cagggggtga cggattcggt gggcgaaacg gtccgcttgg
    16381 gcgcgcggga tttgcggctg cctgttccgg acaaggtgcg cgccggggcc ctgttcgatt
    16441 tttggggcgc cgctccgcgg aaggtccggc ggttctgcga aacacatctc atgaacgatt
    16501 tggtccaccg attggagttg gagaaatgtt gagaggaccg gatttcctgg aggcgtcccc
    16561 catggaactg gcggcgttga gccgtcgacg gcggctgtcg ctgtccgatc cggagctggc
    16621 ggcgatccgg cagcatttca aacgattggg ccggaacccc acggaggcgg agctggagac
    16681 cctggcgcag acttggtcgg aacactgcaa acacaagacc ttccggggga ccatcgacca
    16741 cgtggagagg gatgagttgg gcatggaacg ccggcggcga tatggagacc tgttgaaaga
    16801 gacgatcatg cgggccacgg acgagatccg cgcgccttgg tgcgtttcgg tttttcggga
    16861 caacgccggc atcgtttcgt tcgatggccg ccgcgccgtg gcgttcaaag tggaaacgca
    16921 taaccatccg tcggcgttgg aaccttacgg cggggcgggg acgggtctgg gaggggtgat
    16981 ccgggacgtc ctcggggccg gcctgggggc tcggccgatc gccaatacgg acgttttttg
    17041 tttcgccccg ttggaacaaa aagacgttcc gcgggaaggc ggggctctgt cggcccggcg
    17101 ggtttttcac ggagtggtct cgggggtccg cgattacggg aaccgcatgg ggatccccac
    17161 ggtgaacggc gccgtttgtt ttgatgaggg gttttcccag aacccgctcg ttttctgcgg
    17221 gacggcgggc gtgttgccgg tttccgcggt gcgcaaagag gtgcgccccg gcgaccggat
    17281 cgtcatggtc ggcggtcgcg tggggcggga cggcatccat ggagcgactt tttcttctga
    17341 caacctttcc cacggcatcc ccgccagcgt ggtgcagatc ggcaacccca tcgttcagaa
    17401 aaagatgatg gatgcgctcc tgcaggcgcg cgaaaagggg ctttatcggg cggtcaccga
    17461 ttgcggcgcg ggcggtttgt cttcggcggt gggcgagatg ggggaacaat cgggggcgga
    17521 ggtccaattg gaggacgtgc cgttgaaata cgaggggctg gccccgtggg agatatggtt
    17581 gtcggaatcc caggaacgga tggtgttggc ggtgccgccg cagaatctgg aggcgttgaa
    17641 ggcgttgttt tcggcggaag acgtgctctg tgtcgacatc ggatttttca ggaacgacgg
    17701 ccgtctgcgc gtcctctacg ggcaggaaac gatcgtcgac ctgccggcgg attttctgca
    17761 caacggcgcg ccgcgccggc atttgtcgtc ctcctggtcg ccgctccgca ttccggcgga
    17821 gtggccgcga aaaggcggcc gggcccggcg ggaagaggac gtccgggaaa cccttctgga
    17881 aattttgagt tcgccgacgg tggcgagcaa ggaatggatc gtgcggcagt acgaccacga
    17941 ggtgcagggg cggacggtcc tgaaaccgtt ggtgggtccg gcgggccgcg gaccgggcga
    18001 cgccgcggtg atgcggctgg attgggagac ccacgccggg gtggcgttgg cgaacggctt
    18061 gaatccccgg tacagccgtt gggaccctta ctggatggcg gcgtcggccg tcgatgaggc
    18121 cgtccgcaac ttgatcgccg tgggcgcgag ccgggaccac gtcgcgctgt tggataattt
    18181 ttgtgggggc aacccgaacg aaccgaaggt gttgggggaa ttggtacgcg ccgcgcaggc
    18241 gtgttacgac atcgcccgtg cctacggcac cccgtttatt tcggggaagg acagtctgtt
    18301 caaccgtttc gtcgacaaaa aaggcgcgcg gaaagacatc ccgacgacgc tgttgatctc
    18361 cgccctggcc tggatggacg acgtgcgacg ggccgtcacg atggacgcca aaaaagccgg
    18421 cgaccttatt ttcatcgtcg ggctgacgag ggatgagctg ggcggttcgc attacggcct
    18481 ggtgaaaaac atcgtcgccg ggcgagtgcc gcgtttgtat ccggaggaga cggcgcctct
    18541 ctacgacgcc ctttccgagg cgatggagcg tggctgggtg acggcctgcc atgattgttc
    18601 ggaaggcggg ctgggggtgg cgttggcgga gatggcgttg gccggggaat tggggattta
    18661 cgcggatttg cgccgtgcgc cgctcgacgg ggccgtgtgg gacgattcca gaacggacaa
    18721 agcgcttttt tcggaatcca acggtcggtt tgtggtggag gtcccgccgc ggttccgtcg
    18781 gtatttcctt tcccgtttcg aggggatgcc cgcggcgtgc gtcggcgagg ttcgggaggg
    18841 gcggaaactg cagaccgtcg gtctggaggg gaaatcgcgc gcctgggctc tgtccgacct
    18901 cgccgccgcg tggacggggg gggtgcggcc atgacggtgc gcgcggtggt gcttcgatgc
    18961 gccgggacca actgcgacga agagaccgcc gcggccctcc gccgggcggg cgccgagccg
    19021 tcgcgcgtcc atgtcaaccg gttcctggag ggcggacggc gattgaacga ttacgacctc
    19081 ctcgtcatcc ccggtggttt ttcctacgga gacgacatcg cttccgggaa agtgctggca
    19141 aaccagttgt cccaccggct gaaggatcaa ctggacgaat tcatcgcccg ccggaaaccc
    19201 gtaatcggta tctgcaacgg gttccaggcc cttctgaaag gcggtttttt gccgggccgc
    19261 gccggcgtct cggtgtcggc gacgctgacg gacaacgatt ccgggaagtt cgagtgccgg
    19321 tggaccttcc tccggacgag ttcggggaaa tgcccttttg tgagaggcct gcccgcggtg
    19381 ttccccgccc ccgtggcgca cggcgaaggg aaattcgtgc cggaatcgga ggccgggctc
    19441 cggacgctgg aaaaacaagg gcagatcgtt tttcgctatg tgaacgaacg cggccagtcg
    19501 gacggctatc cgtggaatcc gaacggttcc cggggcgacg tggccgggat atgcaaccgg
    19561 gaggggaacg tgttgggcct catgcctcat ccggaacgct atttttatcc gcagcaacat
    19621 tcccattgga cgaggatccg gccgttgccc gaacacggcg tggggcttca gatattcaag
    19681 aacgccgtcc gttacgcggc gcaggtggcg tcatgagggg ggggacgatg aaaataggcc
    19741 cgcggccctc gttcgcgttg ccgttcgacc ggatgccgga ggagtgcggt gtcttcggta
    19801 tatataacca cgccgaggcg tcgcgactgg cgtatttggg catccaccaa ttgcagcacc
    19861 ggggccagga atcggcgggc atcgtgacca ccgacggcaa gcgtttttat actcacgtgg
    19921 ccatggggct ggtggcggat gtgttcaatc ggccgctcct ggacgatatg aaaggccgca
    19981 ccgccatcgg gcatgtgcga tacggcacct ccggcgtctc ctccctcgtc aacgcgcagc
    20041 ccatcgtggt gacgacctcc cgcggcccca tggcgctggc ccataacggc aatctcacca
    20101 acgccctgct gttgaggcgg cagctggaga aggaaggttc catcttccag acgtcttcgg
    20161 acagcgaggt cattctgcac ctcgtggcca agtcgtcggc gcgggacctg ggggccgccc
    20221 tgaaagaggc tttttcccag gtggaaggag cctactccat cgtgctgttg acgccgaaga
    20281 ccttgttcgt ggcgcgtgat ccctggggcg tgcggccgtt gcacatcggt cggttgaacg
    20341 gttcgttcgt ggtggcctcc gaaacgtgcg ctttcgacat catcaacgcc cggctggtcc
    20401 gggaggtgaa gccgggcgag atcgtggcga tcgacggcca ggggatacgc cccatcgccc
    20461 agctgtcctc cacccggctg gcgcattgta tcttcgagtt cgtttatttt tcccggccgg
    20521 acagccggat cttcggccgc agtgtatatg aggttcgccg tgaactcggc cggcagctgg
    20581 cgcgggagtc gccggtgaag gcggacgtgg tggtggccgt tccggattcg tccagttgcg
    20641 ccgcggtcgg ttacgccgaa cggtcgggat tgcccctgga gctcggattg atccggagcc
    20701 attacgtcgg gcgcaccttc atcgagccga aacaggcgat ccgcgatttc ggcgcccgga
    20761 tgaaatacag cgccgtggcc gacgccgtcc gcgggaaaag gatcgtcctg gtggacgatt
    20821 ccatcgtgcg ggggaccacc agccggaaac tggtgcggat gttgcgccgc gtcggcgtca
    20881 aggaaatcca tctgcggatc agttcccctc ccatcctcgg accctgtttt tacggcatag
    20941 acacgcctga aacgaaagaa ttgatcgcgg cgcgattgtc cgtggaaaag atacgggagt
    21001 atttgcgggc ggacagcctg aaatatcttt ctttgggggg aatgctgaag gccaccaaga
    21061 tgaatcccgg ggatttttgc accgcgtgct ttacggcgcg ttatccgatt ccgctcagga
    21121 ccaacggaac gatcaaatag ccggggacgc gatgtgtcgt ccgagaagag ggagaaattc
    21181 atgaagagat caggaaagaa atcgccgcag gcggccgtgg tgatggggag cgaatcggat
    21241 ttgccggtga tgcgccaggc cgccgagagc ctggaacagt tcggcatccc gttcgagatg
    21301 cacgtccttt cggcccaccg gacgccgcat gagacggtgc ggttcgccgc cggcgcccgt
    21361 cgtcgcggga tccgcgtcat catcgccggc gccgggatgt ccgcccatct tccgggcgtc
    21421 atcgcggcca tcaccgaact gccggtcatc ggcgtgccgg tgtcgggcgg tccgctgcag
    21481 gggcaggatt cgcttttctc catcgtgcag atgccccgcg gggtgccggt ggccaccgtg
    21541 gccatcggca acgcctggaa cgccggaatc ttggcggccc agatcctggg cgccggcggg
    21601 agcggggcgc tcctggaccg cgtggcgcgg tacaaaaagc aactgaagaa aaaggtcctt
    21661 tctagaaaag tcgggctgga cgcgccgcgg gagaaatgaa gccgtgaacg tgttgatcgt
    21721 cggcggcggg gggcgggagc acgcgctcgc ctggagcctg aaacagagcg ctcgagtgga
    21781 ccggctctgg tgttcgccgg ggaacgccgg catcgccgcc cacgccgact gtttgccgct
    21841 ggattttgac gataaacaga aagtggccgc gttccatcga cagaacaaga tcgacctgac
    21901 ggtcgtcggt ccggaacttc cgttggtgaa aggattggcg gatacggtgc ggagtttcgg
    21961 agggctggtg ttcgggccgg gccgcctcgc cgcgcggttg gagggaagca agacgttcgc
    22021 caagagcttc atgaagaccc acggcatacc gacggcggac tttcggattt tttccgagtt
    22081 cgaagaggcc tcggcgttca ttcggtccgg ggagtggccg gagtcgttcc gcgtggtgaa
    22141 ggcggacggg ttggccgccg gcaagggggt gcgtgtctgc gccgaccgca acgaggtctt
    22201 ggaggcggtg gaggagatga tggttcgacg ggccttcggg gccgccggag agaatgtcgt
    22261 tctggaagag atgctggcgg gggaagaact cagcgtcatg gctttttgcg acggtaaaaa
    22321 actcctgccc ctgctgccct gtcgcgatta caagcgggtc ggtgacggca acgtcggccc
    22381 caacacgggc gggatgggcg cgtattcgcc cgtttccgcg atttccgagg ccctttgggc
    22441 ccggatagag aagacggtct tccagccgtt cctgcgcggg atccggagcg agtggctcga
    22501 ttactgcggg gccatttatt ttggattgat gctgaccccg gaggggcccc gggtgttgga
    22561 attcaacgtg cgcttcggcg atccggagac gcaggccgtc ctgccgctga tcggcaacga
    22621 cctgttcgaa ctcatctcgg cgacggccct cggcgaattg ggggatgtgg cgcttcgtcg
    22681 gcgggcccag gcctcggtct gcgtcgtgct ggcgtcgaag gggtatcccg gcgactttga
    22741 gacgggcaaa cccatcagcg gtttggaacc gtgggcggac gccccggacg tggcggtttt
    22801 tcacgccggc acgcgtcggc gggattctca gatcgagacc gccggcggcc gtgttctggg
    22861 cgtgacggcc tgggggcccg atctggccaa ggctcgtgcg cgcgcctatg aggcggcgga
    22921 taaaataaaa ttcgaaaacg tctgttttcg aagagacatc gcctcggagg gatgaacgat
    22981 gaaaaaaaag atacggacgg cggctcaggt caaatcgctc ttcgaccggc ttgccgccga
    23041 catccggaag acggtaaagg aacctcgcgc cgactgggtt ttcgtcggca tccagaggcg
    23101 cggggtgccg ttggcccggc gactttcggc ccttttggcc gcgcccggcg ggcccgccgc
    23161 ggtcggcacg ctggatatca cgttttaccg cgacgattca caggccgtgc cgctgcatcc
    23221 ggtggtgcat gacacggaac ttcccttcga tgtgacgggg aagacggttt ttatcgtcga
    23281 cgacgttctt ttcaccggtc gcacggtccg gtgcgcgttg gatgagttga tggatttcgg
    23341 ccggccccgg cgggtctatc tgggggtcct gatcgaccgc ggtcatcggg aactccccat
    23401 ccaggcggat ttcgtcggcg aggcccttcg gacggattgc gacgagacca tcgatgtcca
    23461 tttgagggaa accgacgggg aagacgcggt gtggcgcagc ggtcggccgg agaagccggc
    23521 atcaaaacgg aagacggagg ttgcccgttg aaagacctga tcggacttga gcaattgtcg
    23581 aagacccaga tagagacgat cctgaagacg ac
//
LOCUS       DAOTFD010000019        23543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1427_length_23543_cov_4.785124, whole genome shotgun sequence.
ACCESSION   DAOTFD010000019 DAOTFD010000000
VERSION     DAOTFD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 23543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23543
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_23543_cov_4.785124"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="P5079_02345"
     CDS             <1..811
                     /locus_tag="P5079_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRY28859.1"
                     /translation="VPVGESVLLRHRPWSVALDDFNIDRYLDTGAPRRYVSRVRVVQN
                     DRPLADKKILVNDPLDVQGVRFYQASWGMTDSFRSVTLETPSSSLLDVARGQKIPLGG
                     TNYRLTVEAVLPDFFVDEAGRADTRGFFPNNPAALVHFIDGEEAVASFWLPARSPETA
                     FRVAAGERLFPVSSPPLRLRHIEPVLFSGIQVTYDPGVGVVGLGCLLLLSGLFLHFYP
                     RRREFLFRFKTNGGGTRISIGAWSSRDAPELAVETQRMAEFWKGGTRDEVD"
     gene            798..1718
                     /gene="ccsB"
                     /locus_tag="P5079_02350"
     CDS             798..1718
                     /gene="ccsB"
                     /locus_tag="P5079_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRY28860.1"
                     /translation="MRLIEAWTFGVAFAGYAVGLFFYVGYALSRREMWGRWGRRVLIF
                     AVGCQIVSLVTRVMAARTVFGQNWYVPWSNWFESFSFFAFLIVVGFLLIETSSSLPIL
                     GVFVVPFAGLALLMSFIEPLLSGQASIVRAADLGLLLSSARAIPQLPPALQSYWMAVH
                     VPAMFVAYAAFTNAFGLGLAYLIQERQIKSRRPAEFIYRLPSLEELDRLIYKVIFLAF
                     PVLTLGIILGARWAYEAWGRYWGWDPKETWALITWLIYLAYLHVRLAGGWRGRRTAYF
                     ALFGFAVVLFTYVGVNYLSVLHGFLSGVGR"
     gene            1740..2396
                     /locus_tag="P5079_02355"
     CDS             1740..2396
                     /locus_tag="P5079_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28861.1"
                     /translation="MKEGLSKSFFLWSRRSAAALFCAVPALSVLAQENSSLPARTIDV
                     RVENVVRLGASGIVVQQRQLYGNKFLEVVWEMIDRNKDGKISKAERKQFPELGKSMYV
                     NFSALRLNWMVQIPEKIEVDLSAWPSRKPKDVEKAPPLPVVLTVSYKYEPTALDLVEL
                     MMPNFNAHDISCRFMLDPVFRALRSNLGKIPSDQSVTDMAMTDGVPGSFSVIVHKRVD
                     "
     gene            2393..3493
                     /gene="hemA"
                     /locus_tag="P5079_02360"
     CDS             2393..3493
                     /gene="hemA"
                     /locus_tag="P5079_02360"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRY28862.1"
                     /translation="MKKLLLIGVSHKTAPVERRERLSLSEAALPDFLGRISGAPAVAE
                     AVVLSTCNRLEVYVLTESPSAARELIEKELAARWGEGAPPWYVREDLAAVRHLFHVAA
                     GLDSLVIGEGEILGQVKRAYEAALGARGTGKFTNVLFQRALYVGKLVRAETRISEGAT
                     SSASVAVSLAGKIFGDLTEKNILIVGAGKMAELSARNLLSQKAARLTVANRTLDKATE
                     LAGPLGGRAVPLEDLPEELLVADIVICSTGAPTPLVRAADVRDIMARRRGRALFFIDI
                     AVPRDVESEVNGIENVYLYNIDDLQAIVSETVGRRRSEIEKATLLADRKAEEFCRCCE
                     SWQAGRPAPFAHSDFLSRSSVGGPAADATFLE"
     gene            3486..4409
                     /gene="hemC"
                     /locus_tag="P5079_02365"
     CDS             3486..4409
                     /gene="hemC"
                     /locus_tag="P5079_02365"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRY28863.1"
                     /translation="MNKIRLGTRGSALALAQAGIVKDRLRAVEPSLEIDIVPIRTAGD
                     RWTGTSFPESGVKGLFVKEIQESLRAGRVDAAVHSLKDLPVLAAEGLRLVAVLEREDP
                     RDCVVSRQGGPLSGLPAGSVVGTASPRRQAQLRAAYPTLRFENIRGNLDTRIQKTREG
                     GYDAVVVARAGLRRLNRDSEISEILPLDVVLPAPGQGCLAVEVRAGDGRMERWAAALN
                     HDASFWTASAERSFLAGLGGGCRVPVAACALLADDGGMTLQGLVISADGRRQVRGTVS
                     GPSASAEELGQSLARKLLADGAAPLLAAVSE"
     gene            4418..6004
                     /gene="cobA"
                     /locus_tag="P5079_02370"
     CDS             4418..6004
                     /gene="cobA"
                     /locus_tag="P5079_02370"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRY28864.1"
                     /translation="MDEQRRRPGKVYLIGAGPGRPDLLTLRGAECLSQADVVVRDASV
                     SERVLEHCRPETPVLLTRRGIRRRPMGQAEINRRLVRLARSGKIVARLKVGDPFFFSR
                     GAEEAEFLARRHVPFEVVPGVSSVTAVPAYAGVPITHREYASVLTVVSGPAGRENTYW
                     AGPGQKNVASVRWEALPPQGTLVILMGVGELRGIAQRLMLNGWLASTPVLVARWGTLP
                     AQKIVEGVLGDIAPKAEREGMSAPAVIVVGKVAAFRRRLGWFERLPLFGRTVLVTRAA
                     SRSDALTALLENEGARVLVSPAVRFEPVKPAPRWRRCLRNISRYDGVLFTSATTVRIF
                     SEHWREMSRDWPVGVAVYAVGGKTAEQLVRNGFPVHGIGKDAAAGDLVDVLGETEGRR
                     YLFPRAAEGRDELVEKLRAAGASLDLWPLYRTRPVKMEAGMRSSLLAGTVDGVLFASP
                     SAVGSVLGSFSPRQRRKIFSRTRLFSIGPTTTRALKDFGMRRVHQATRSTMESLAVTV
                     QEKLKIRRRNEGGAYENPFR"
     gene            5985..6800
                     /locus_tag="P5079_02375"
     CDS             5985..6800
                     /locus_tag="P5079_02375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY28865.1"
                     /translation="MKIRSGDVAAETLRRALMRALCSAGDVLRGFSRRPIAVTLKGPV
                     NPVTAADRAAEKKIVKILRGRFPEHGFLTEESPPRRTASPYRWVVDPLDGTVNYAHGV
                     PHACVSIAVEKNGRVLMGGILDPFRRELFFAARGRGSRLNGRPIRVSRKKRLIESLLV
                     TGFPYDRYKKSRYYLRFYNEFMKRTQGLRRFGAAALDMAYVAAGRFDGYWEFNLKPWD
                     SAAGWVLVEEAGGRVSDFRGTGYSLADTRQTLCSNGFLHDAMRKILRTGASGL"
     gene            6849..7835
                     /gene="hemB"
                     /locus_tag="P5079_02380"
     CDS             6849..7835
                     /gene="hemB"
                     /locus_tag="P5079_02380"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRY28866.1"
                     /translation="MTFPISRPRRLRKSASLRRLLRETRISPASLVEPFFVCPGKNVR
                     QPISAMPGICRFSVDHLLREARESHRLGIPAVLLFGVPREKDPLGKEAYARDGIVPTA
                     VRALKDALPDLTVITDLCFCEYTDHGHCGVVKKSGRSFVLDNDATLDLMARTAVVQAD
                     AGADGVAPSGMIDGGVRTIRRALDEDGKKDTFLLAYTAKYASAFYGPFREAAASAPLF
                     GDRRTYQMDPANAREALREAALDVKEGADIVMVKPALAYLDVLRLLKDRFSVPVAAYN
                     VSGEYSMIKAAAAKGWIDGAAAALEVLTSIKRAGADVIVTYHAREAARGMKP"
     gene            7832..8593
                     /locus_tag="P5079_02385"
     CDS             7832..8593
                     /locus_tag="P5079_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY28867.1"
                     /translation="MNRSVLRWMLCLAMAALPAACARRQMVKPPGNPEWGVRIVWYGH
                     SCFSFEDSVERKFLVDPFDETVGYDIPWMKSDVLMVTHEHFDHDFIRRAERCEVVNTT
                     GVITAAGVEIEGGLGFHDAEGGARHGVTRFYVWEMGGLRFAHLGDLGDPNLSEAQKEP
                     LRNVDVLFIPVGGKTTLDGAAAAALVKELQPRLAVPMHYGNPRVRFFEFEPVESFLSH
                     FDDVVPLADTDFLVRKDDLPAGLTIYVPALPPARR"
     gene            8651..9928
                     /gene="hemL"
                     /locus_tag="P5079_02390"
     CDS             8651..9928
                     /gene="hemL"
                     /locus_tag="P5079_02390"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRY28868.1"
                     /translation="MSKSKELYRRAQTLLVAGVNSPVRAFHAVGGAPIFMARGRGSEM
                     VDVDGKRYVDYLGSWGPLIFGHAPPPIIAAAEKALRLGATFGTPVESEARLAELVRAA
                     FPSMELMRFVSSGTEACMAALRLARGYTGRRLIVKFAGCYHGHSDGLLVSAGSGAATF
                     GHPTSAGVPREIARLTLVLPYNDATRVRKAFRSHGRDIAAVIVEPVAGNMGVVPPEKQ
                     FLRVLREETKRHGALLVFDEVITGFRLAWGGAQTVYGVRPDLTCLGKIVGGGFPVGAF
                     GGAAKIMRRLAPLGPVYQAGTLSGNPVAMAAGIATLELLRRTRPYASLARQTARLSAG
                     IGALAAPRGLAVTVNRVGSMFTVFFTDRPVRDGFSAMRSDARRYARFFQGLLKRGVYF
                     PPAQLEAAFVSCAHTAADLEKTLTAVAETFEEL"
     gene            9928..10920
                     /locus_tag="P5079_02395"
     CDS             9928..10920
                     /locus_tag="P5079_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879161.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRY28869.1"
                     /translation="MEILVLSEADVQSLFSVKEAIPLVEHAFLELARGAAQMPPKVYL
                     SFPEFQGDLRAMPAYMPSTGAGSGKPYAAVKVVNSHGRNPERGLPAVAATLVLNDPET
                     GLPLAFLGAGRLTDRRTGAAGAVAARCLARPESAVVGLVGCGRQAMTQLEGLRNVFQI
                     KKVLAAGRTFEEARQFCSGLKGEDLEVVPCEDVRRACAADILVTTTPSRSPVVRAEWV
                     RPGTHINAIGADAPGKQELETALLRSARVVVDWPEQAFHSGEVNVPFAAGALKKEQVV
                     GHLGEILLGVKKGRTSPSDITVFDSTGLAVQDLAAASWVYEKALAEKKGRKIVI"
     gene            10931..12148
                     /locus_tag="P5079_02400"
     CDS             10931..12148
                     /locus_tag="P5079_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRY28870.1"
                     /translation="MAEIRPFRAFHHHPSVRRELRRLVCPPYDIISSDDQKRLLRKHP
                     ENFVRIELPSGRPPGKYRRAAALWRRWVERGFIIRDFSPAFYAYETEFVSRLARRRLR
                     RRGFFAALRLRPWGQGVHPHEKTLSTPKADRFKLFKSMRLQTSPIQCLFWDRSRLATR
                     LLARVARGGAWREFSDTEARHRFWRMGDAKTIRQLQKVLAASDVVIADGHHRYETSRA
                     YGAWCRSRGQATESSRYVMAYFSPTSDAGLEILPTHRAVGADKAHFVNLDQWGKMEPV
                     RDYAHLQRRLRSSRAAAVGVYREGKYYFYRFTAVPASLRRTPFENSSVALLHTGPLAG
                     LGREDFFFSRRAEEALRWARRSRGWGFILTPSTVEEVMRVALFGLVMPPKSTYFYPKI
                     PSGILGRSLSGPL"
     gene            12196..12720
                     /locus_tag="P5079_02405"
     CDS             12196..12720
                     /locus_tag="P5079_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRY28871.1"
                     /translation="MSGLDIGIVLLTVAGIFFGLKIGVISACFNILAGFAGTWVASRF
                     YVVLSGYLPRPAFSYFLLFIIVAAALVAAGILISQALESYFLGLVDKFLGALLGMVLS
                     LLFSSVVLFPLVLEQTPAIKNLMRRSAFAPYVIRTTQRYGRLAAQDVWARVEPLLESD
                     DLRRVRKLLENSPR"
     gene            12731..>13063
                     /locus_tag="P5079_02410"
     CDS             12731..>13063
                     /locus_tag="P5079_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28872.1"
                     /translation="MPLVKRKTGVERQVSGEASVVARTLWDFVGASPWRFWLEGGPEG
                     TVAIAWGRPLWEFRGKNGVGFLRTGRKIRRFSFSDPLPVLREQLRFEKTDDDRGASLV
                     DMGMRCGRL"
     assembly_gap    13064..13073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13392..14192
                     /locus_tag="P5079_02415"
     CDS             13392..14192
                     /locus_tag="P5079_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRY28873.1"
                     /translation="MAGVRRILEYIAAGDIYQANLSHRLEFPWPAGTDPSVYFHALRR
                     INPSPYAAFLGFPDFSIASCSPELLLRARGRRVETRPIAGTRPRGHNPSADERLSGEL
                     VLNAKERAEHIMLVDLERNDLGRVCHPGSVRVSEQMVLEKYSHVMHIVSNVQGRLRSG
                     ADALDALQALFPGGTITGCPKIRAMEILDELERVPRGPFFGSAGRISASGDVDLNILI
                     RTALIRRGRLALRVGSGIVADSSPSREYEETLHKARALVNAYRMCNNS"
     gene            14217..15113
                     /locus_tag="P5079_02420"
     CDS             14217..15113
                     /locus_tag="P5079_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRY28874.1"
                     /translation="MPKKIWVNGRLVDPRRPAVSVFDRGFLYGDGVYETIRVYDGKPF
                     QLEPHFQRLARSLRGIGMRVPFGRAVLGDAVASVMAANRLADGVVRLTVTRGEGALGF
                     DPASCRRPTVVLLAVPFASHPAVHYEKGVRAVFVRLRRNAKNALDPSIKSLNNLNNIL
                     AKMESMKKGGQEALMLNQEGYLTEGTISNIFFISGGVLKTPSLDCGLLDGVTRSVVLS
                     LAKRRSLAVWEGRCRPRHLFSAEEVFLTSTTLEVMPVTSVVDEQGRLRRIGSGQVGEW
                     SGRLLRDYRRLITKATGKKFKI"
     gene            15110..15943
                     /locus_tag="P5079_02425"
     CDS             15110..15943
                     /locus_tag="P5079_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28875.1"
                     /translation="MKRRTGILVALFLLGSLGTAAEENSSPQSQKRAVSVEASPPLWT
                     EDPDPAPRPRRARPRERSAAPRLFWQVDFWTGNDAFEEDEEAARRFSASLLSIGYDAA
                     QGSVQNSGGLGLRFGLLFPTMTGWDIGGSAGYVAGPTQDVTVIALSRAAGDGMLVEEA
                     ETDFYRFLFEAQKEVPLSRWVGLRLRGGAGWAAGKMRVRDQYTGTFVSLLGYPASAEY
                     VNGWGGFTWEVMPSFVFRGNWANFEVGLGWAGFPKMEETDDLNEFAWNPLGFRLGLEF
                     R"
     gene            complement(16406..17173)
                     /locus_tag="P5079_02430"
     CDS             complement(16406..17173)
                     /locus_tag="P5079_02430"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY28876.1"
                     /translation="MKHFQKILPVLLFLTLPALALAGDATRIPLIGEEAPAFTAETTQ
                     GPINFPADYKGKWVILFSHPADFTPVCTTEFMTFASMSNEFKALNCELIGLSIDSIYS
                     HIAWLRTIKEKAKYKNLQNVEVLFPLIADLKMEVAKKYGMLQPTASDTKAVRAVFFID
                     PSSKIRALIYYPLSNGRNFQELKRLLVAMQTSDAHAIATPADWQPGDDVIVPPPGSCG
                     TAKDRVEKSGADYYCHDWFLCFKKLPKENLSAPAKKK"
     gene            17276..19078
                     /locus_tag="P5079_02435"
     CDS             17276..19078
                     /locus_tag="P5079_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRY28877.1"
                     /translation="MERKGSRTAWLPITRDELPALGWDTPDVVLVTGDAYVDHPAFGT
                     AVIGRVLEQAGVRVAVIPQPNWRDDLRDFRKFGRPRLFFGVTSGNMDSMMNHYTAHRR
                     RRADDAYTPGGAAGFRPDRAVTVYTKILKSLYPDVPVVLGGIEASLRRLTHYDYWSDG
                     LRPSVLQESGADLLVHGMGEEAIRRIAELLMRGVPFSSLKTIPQTAFLMAADEPLPKN
                     SRWETLALPSHEECAGDKKEFLKSFKLFETESNRLNARRLTQRLADRRIVVNPPFPPP
                     SAKELDAIYDLPFTRLPHPKYQKRGPVPAFEMIKFSITLHRGCFGGCAFCTISAHQGK
                     WISSRSKESVVREAGAVADMPGFAGVISDLGGPTANMYGMRGKDTALCAHCARSSCLF
                     PAICENLNTSHAALLELYRAVEGHSKVKKAFVSSGVRHDLALKDESYRETLAARHVSG
                     RLKLAPEHTEERVLKLMRKPSFEAFLKFKKWFDGFCARRGLRQEILPYFISSHPGGTE
                     EDMARLAVRTKELGYRLEQVQDFTPTPLTLASVIYYTGLDPDTLKPVPVARAPQEKKA
                     QKDFFFWHKPEMRAVLKRALLRLGRGDLIRRLFG"
     gene            19175..20278
                     /gene="ugpC"
                     /locus_tag="P5079_02440"
     CDS             19175..20278
                     /gene="ugpC"
                     /locus_tag="P5079_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY28878.1"
                     /translation="MARVDLKNVAKKFGDHYVVKNVNLEIPDKEFFILVGPSGCGKST
                     TLRMIAGLEEASEGTVSINGQVVNDVPPKDRDIAMVFQNYALYPHMTVYENMAFGLKL
                     RRYPKSEIDRRVREAAEILNLGPLLQRKPKALSGGQRQRVAVGRAIVRKPHVFLFDEP
                     LSNLDAKLRVQMRVELSKLHDRLQTTMIYVTHDQVEAMTMGDRICVMKDGEIQQVAPP
                     LELYHKPANQFVAGFIGNPPMNFFPVRIEKNKDGVWLRHEAFSLAAPAATADKIRPRV
                     GQEAVLGIRPEDIYDRLFYSSQVPEGSTVKAVVEVLEPMGSEIYLYLKAGKTDVVARM
                     PPYVKTKLHQEIELVFNLERLHLFDKETGVSLL"
     gene            20290..20673
                     /locus_tag="P5079_02445"
     CDS             20290..20673
                     /locus_tag="P5079_02445"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HRY28879.1"
                     /translation="MFWKALTDVVVFLHGFWVVAVVVGPVWAWKNKRFRAVHLGMLWW
                     TFFVLASGIYCPVSHLENSFRMHYDPSGSYPGGFVVHYVNRMTSWDLTQPQVVSAMIF
                     WTFLWTAVYVFLWTKKAKPSPESRK"
     gene            20683..21939
                     /locus_tag="P5079_02450"
     CDS             20683..21939
                     /locus_tag="P5079_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28880.1"
                     /translation="MKMKSRLFRVAFFFFAAGTISIPPAGAVGPDYSDIYNLDAEYEL
                     DRLMGDIPWEWRDRFRSSVSGFQGTGGSLNIRHLYSEQVFKARFPLLQDRLWFRILDK
                     RNRGLEREEVETHLEFEYSPAPRWYLSFLGAPALHKSDVVVGAAARWGRDEGRSVRFA
                     YLWPGFDTNYAFHNRSINEGYEEFYRRFPQEARLSAVWIRSPLSFFIEGRHRRTSELE
                     HQEFSPPRTRFISGDGEAEAVWDVRYVAGRWAWGTEGSLWRARESLRYEPAAAALDQT
                     LIRERSAARLTLRRTLGAGWAVRVGGGMVGSRGHLRYPGQPLSDRAYRIYDRIGYVAA
                     DHAVTARFGVEAGYFYDRQGRKSVETGNVAIMEKNSQNRGKLAIRYDFSENASLRVIS
                     AIELDQNQSENFASFDGGTIQFQTTF"
     gene            21990..22529
                     /locus_tag="P5079_02455"
     CDS             21990..22529
                     /locus_tag="P5079_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRY28881.1"
                     /translation="MCGTLLSSAPSQKQGHRRYREAPASELSSAGNYRSTGRVVKLRS
                     AAAESFRAMVAAASAEGVRLIPISGFRTVAYQKVLFRRACRRYGSEKKAARWVAPPGH
                     SEHHTGWSLDLGEESSPETDVDASFENTRAFRWLEENASRFGFELSFSKDNPQGIGYE
                     PWHWRYVGAEAPKKLFSTP"
     gene            complement(22649..22990)
                     /locus_tag="P5079_02460"
     CDS             complement(22649..22990)
                     /locus_tag="P5079_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRY28882.1"
                     /translation="MKYIVAIIQPDRVDEVLKRLEENEIHLVTATTVLGRGRQKGISE
                     VYRSHKEAGSLLRKVRLDIAVNDAFVEPAMDAIVKGARTGHIGDGKIFVLDLSECVRI
                     RTGERGSVAIG"
     gene            complement(23001..>23543)
                     /locus_tag="P5079_02465"
     CDS             complement(23001..>23543)
                     /locus_tag="P5079_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRY28883.1"
                     /translation="AFVVTHIAAAAAGLTWALLDWVFNSKPTVLGTITGAVGGLVAIT
                     PAAGFVNVAGALMIGLGVSLICYVAVAVIKPRLGYDDSLDAFGVHGIGGMWGAVATGL
                     FATKMVNPAGADGLFYGNPLQLLIQLKATLVTAVYSFVVTFVLLKAVKALVGVRAEAD
                     HERIGLDLTDHREAAYTLLD"
BASE COUNT         4013 a         6870 c         7643 g         5007 t
ORIGIN      
        1 ggtgccggtc ggcgaatcgg ttctcctgcg gcaccggccc tggtcggtgg cgctggacga
       61 tttcaacatc gaccgatacc ttgacaccgg cgcgccgcga cggtacgtca gccgggttcg
      121 agtggttcaa aacgaccgtc cgttggcgga taaaaagatt cttgtgaacg acccgctgga
      181 cgtccagggc gtccggttct atcaggcctc gtgggggatg accgactcct ttcgttccgt
      241 cacgctggag accccgtctt cctctctctt ggatgtcgcg agggggcaaa aaattccgct
      301 gggggggacg aattaccgtt tgacggtgga ggcggtcttg ccggatttct ttgtcgatga
      361 agcggggcgg gccgacacgc gcggtttttt tccgaacaat cccgccgccc tggtgcattt
      421 tatcgacggg gaagaggccg tcgcctcttt ttggttgccg gcccgttctc ccgagacggc
      481 gttccgggtg gcggccggag agaggttgtt tccggtctcc tccccgccgt tgcggcttcg
      541 tcatatagag ccggtgttgt tttccggaat ccaagtgacc tacgatcccg gggtgggggt
      601 cgtgggactg ggctgtctgc tgttgttgtc cggcctgttt ttgcattttt atccacggcg
      661 gagggagttc ctgtttcgtt tcaagaccaa cggaggggga acgcggattt ccataggggc
      721 ttggtcgtcc cgtgacgcgc cggaactggc tgtggagacg caaaggatgg cggagttctg
      781 gaaaggagga acccgtgatg aggttgattg aggcatggac gttcggcgtg gcgtttgccg
      841 gatacgccgt tggtctcttt ttctacgtcg gatacgcctt gtcccgcagg gagatgtggg
      901 gccggtgggg acgtcgcgtc ctgatttttg cggtgggatg ccaaatcgtt tcccttgtga
      961 cgcgcgtcat ggcggcacgg accgttttcg gtcagaattg gtatgtgcca tggagcaatt
     1021 ggttcgaaag cttctcgttc ttcgcctttc tgatcgtcgt cgggttcctt ctgatcgaaa
     1081 cgtcgtcgtc cctgcccatc ctcggcgttt tcgtggttcc tttcgccggc ctggccctgt
     1141 tgatgtcgtt catcgagccg ctcctttccg ggcaagcgag catcgtgcgg gcggcggact
     1201 tggggctttt gctctcttcc gcgcgcgcca tcccgcagct tcctccggcg ctgcagtcct
     1261 attggatggc ggtccacgtg ccggcgatgt tcgtggccta tgcggcgttc accaatgctt
     1321 tcggtctcgg tctggcgtat ttgatccagg aaaggcagat caaatccagg cgtcccgcgg
     1381 agtttattta tcggttgccg tctttggagg agctggaccg gctgatttac aaggtcatct
     1441 tcctggcgtt tcccgtcctg accctgggaa tcattctggg agctcggtgg gcctacgagg
     1501 cgtgggggag gtattggggc tgggacccga aagaaacgtg ggctttgatc acgtggctga
     1561 tctatcttgc gtacctgcac gtccggctgg cggggggctg gcgtggccgt cgcacggcct
     1621 atttcgctct tttcggtttt gcggtggtcc tgtttaccta tgtcggcgtc aattatctgt
     1681 ccgtgttgca cggtttttta tccggcgttg gccggtaaat ccatcaacgg aggaagaaca
     1741 tgaaagaggg attatcgaaa tcttttttct tgtggagccg ccggtcggcg gcggcgttgt
     1801 tttgcgccgt tccagcgctg tcggtcttgg cgcaggaaaa ttcttccctc ccggcccgca
     1861 cgatcgatgt gcgggtggaa aacgtcgtcc ggctcggcgc ttccgggatc gtcgttcaac
     1921 agcggcagct ctatgggaat aaattcctgg aagtcgtctg ggagatgatc gatcggaaca
     1981 aagacgggaa aatatcgaag gcggaaagaa aacagttccc ggaactgggg aaatccatgt
     2041 acgtcaattt ttccgccctg agactcaatt ggatggtgca gataccggaa aaaatagagg
     2101 tggatctgtc ggcctggccg tcccgcaagc cgaaggacgt ggagaaggcc cctcccctgc
     2161 ccgtcgtcct gacggtttct tataaatacg agcccacggc gttggacctg gtggagttga
     2221 tgatgccgaa tttcaatgcg cacgacattt cctgccggtt catgctcgat ccggttttcc
     2281 gcgccctgcg ctccaacctg gggaaaatcc cgtcggacca gtccgtgacg gacatggcga
     2341 tgacggacgg agtgccggga tcgttctccg tgatcgtgca taagcgggtc gattgaaaaa
     2401 attacttctc atcggcgttt cgcataagac cgccccggtc gagaggcggg aacggctgtc
     2461 tctttccgag gcggccctcc cggatttcct cgggcggatt tccggcgctc ccgccgtggc
     2521 cgaggccgtc gtcctttcca cgtgcaatcg gttggaggtg tatgttttga cggagtctcc
     2581 gtcggcggcc cgcgaactca tcgagaaaga actcgccgcg cgatggggag agggggcgcc
     2641 gccctggtat gtccgggagg atctcgccgc cgtccgacac cttttccacg tggccgccgg
     2701 ccttgattcc ctggtgatcg gggaggggga aatattggga caggtcaaac gggcctacga
     2761 ggcggcattg ggggcccggg ggacgggcaa attcaccaat gtcctcttcc aacgggctct
     2821 ctatgtcggt aaactggtgc gcgccgagac gcggatatcc gaaggggcca cgtcctcggc
     2881 gagcgtggcg gtgtcgctgg ccggaaaaat attcggggat ttgacggaga aaaatatcct
     2941 catcgtagga gcggggaaaa tggcggaact ttccgcgcgg aacctgttga gccagaaagc
     3001 ggcgcgactg accgtggcga accgtaccct ggacaaggcg acggaactgg ccggcccctt
     3061 gggcggccgt gccgttccgc tcgaggattt gccggaggaa cttctcgtcg ccgacatcgt
     3121 catctgttcc acgggcgccc cgacgccgct ggttcgcgcc gcggacgtgc gcgacatcat
     3181 ggcccgccgc cgcgggaggg ccctgttctt catcgatatc gccgtgcccc gtgacgtcga
     3241 atcggaggtg aatgggattg aaaacgttta tctttacaac atcgatgacc tgcaggcgat
     3301 cgtttccgaa acggtcggcc gccggcgttc cgaaatagaa aaggcgacgc tcttggcgga
     3361 ccggaaagcc gaagagttct gccggtgttg cgagtcctgg caggccggcc gtccggctcc
     3421 gttcgcccat tccgatttcc tttcccggtc ttccgtgggc ggccccgcgg ccgacgcgac
     3481 gttccttgaa taagatccgg ctggggaccc gcggcagcgc gctggcgttg gcgcaggccg
     3541 gaatcgtcaa ggaccggctc cgggcggtgg agccctctct cgaaatcgac atcgtgccga
     3601 tccggacggc cggagaccga tggacgggaa cgtcgttccc ggaaagcggc gtcaaaggcc
     3661 tgttcgtcaa ggagatccag gagtcgctcc gggcgggacg ggtggatgcg gcggtgcact
     3721 ctttgaagga tcttccggtt ttggctgcgg aggggcttcg cctggtcgcc gttctggaac
     3781 gagaggatcc gcgcgactgt gtcgtttccc gccaaggagg gccgttgtcg ggtcttcccg
     3841 ccgggtctgt cgtcgggacc gcttctcccc gccggcaggc gcagctgcgc gcggcctatc
     3901 cgacgttgag atttgaaaat ataagaggga acctggatac ccgtatccaa aaaacgcggg
     3961 agggggggta tgacgcggtt gttgtggccc gtgccggtct tcggcggctg aaccgcgaca
     4021 gtgagatatc ggagattttg ccgctcgacg tggttttgcc ggccccggga cagggatgtc
     4081 tggccgtgga agtccgcgcg ggggacggcc ggatggaacg atgggcggcc gcattgaacc
     4141 acgacgccag tttttggacg gcttccgccg aacgttcgtt tctggcgggg ctcggcggcg
     4201 ggtgccgcgt gccggtggcc gcctgtgccc tcctcgccga cgacggcggg atgacgttgc
     4261 aggggctcgt catttccgcc gacggccgcc ggcaggtgcg cgggaccgtt tcggggcctt
     4321 cggcctcggc ggaagaactg ggacaatcgc tggcgcggaa acttttggcc gacggcgccg
     4381 ccccgctttt ggcggctgtt tcggagtgag ggagcgcatg gacgagcaga gacgacgacc
     4441 cgggaaggtc tatttgatcg gggccggccc gggccggccc gatttgctga cgctgcgcgg
     4501 cgcggaatgt ctgtcgcagg cggatgtcgt cgtccgcgac gcctcggttt ctgaaagggt
     4561 attggagcat tgccggccgg aaacccccgt ccttctgacg cgccgcggaa tccgccggcg
     4621 gcccatggga caggcggaga tcaaccgccg tctcgtgcgg ctggcacgct ctgggaaaat
     4681 cgtcgcccga ttgaaagtcg gggacccgtt tttcttcagc cgcggcgccg aagaagccga
     4741 attccttgcc cggcggcatg ttcctttcga ggtggttccc ggcgtttctt cggtcacggc
     4801 ggttcccgcc tatgcgggcg ttcccatcac gcatcgcgaa tacgcctccg tgttgaccgt
     4861 cgtctccggt cccgccggac gggagaacac ctattgggcc ggtccgggtc aaaaaaatgt
     4921 cgcctcggtc cggtgggagg ctttgccgcc ccaggggaca ttggtgatcc tcatgggcgt
     4981 gggagagttg cggggaatcg ctcagagatt gatgctcaac ggttggctcg cttcgacccc
     5041 cgttctcgtc gctcgatggg gcacgttacc ggcccaaaaa atcgtcgagg gggtcctcgg
     5101 ggacatcgct ccgaaggccg agcgggaagg gatgtccgct ccggcggtga tcgtggtggg
     5161 aaaagtggcg gcgtttcgcc gccgattggg ctggttcgag cggttgcctt tgttcggtcg
     5221 gaccgttctg gtgacgcgcg cggcctcgcg gtcggacgcc ctcacggctc ttttggagaa
     5281 cgagggggcg cgcgtccttg tttcgcccgc ggtgcggttt gaaccggtga aaccggcgcc
     5341 tcgttggcgg cggtgtttga ggaatatttc ccgatacgac ggggtccttt tcaccagcgc
     5401 taccaccgtc cggatttttt ccgaacattg gcgggagatg tcgcgcgatt ggcccgtcgg
     5461 cgtcgccgtc tacgcggtgg ggggtaagac cgccgaacag ctcgtccgga acggtttccc
     5521 ggtgcacggc atcggcaagg acgccgccgc gggggacctg gtcgatgttt tgggggaaac
     5581 agagggaagg cgatatttgt tcccccgggc cgcggaggga agggacgaac tggtggaaaa
     5641 attacgcgcc gccggggcgt cgttggacct gtggccgctc taccgtacgc ggccggtgaa
     5701 gatggaggcc gggatgagaa gttcgttgct ggccggaacc gtggacggcg tcctgttcgc
     5761 ctctccgtcg gccgtgggat ccgtcctggg ttctttttct ccccggcagc ggcggaagat
     5821 tttttccaga acgaggctgt tttccatcgg gccgaccacg acgcgggcgc tgaaagattt
     5881 cggaatgcgc cgggtgcatc aggcgactcg gtccacgatg gagagcctcg ccgtgacggt
     5941 tcaggaaaaa ctcaaaatcc gtcggcgaaa tgaggggggc gcgtatgaaa atccgttccg
     6001 gtgatgtcgc ggcggagact cttcgccgcg ctttgatgcg ggcgctctgt tccgccggcg
     6061 atgttttgcg gggtttcagc cgccgcccga tcgccgtcac cctgaaaggg ccggtcaacc
     6121 ccgtgacggc cgccgaccga gcggcggaaa aaaagattgt gaaaatattg cgcggccggt
     6181 tccccgaaca cggtttcctc accgaggagt ctcccccgcg gcgcaccgcc tccccttatc
     6241 gttgggtggt cgatcccctg gacggcaccg tcaattatgc gcacggggtg cctcacgcct
     6301 gtgtctccat cgccgtggag aaaaacggcc gggtcttgat gggtgggata ctcgacccgt
     6361 tccgaaggga gcttttcttc gccgcccgtg gacggggctc ccggttgaac ggtcgtccga
     6421 tccgggtctc acggaagaag cggctgatcg aaagcttgtt ggtgacgggg ttcccctacg
     6481 accgctataa gaagtcccgt tattatctcc ggttctacaa tgaattcatg aagaggacgc
     6541 agggtctccg ccggttcggt gccgcggctc tcgatatggc ctacgtcgcc gccggccggt
     6601 tcgacggtta ttgggagttc aacttgaagc cttgggattc cgccgcggga tgggtgttgg
     6661 tggaggaggc ggggggccgc gtgagtgatt ttcgcgggac aggatattcc ctggcagata
     6721 cccgccagac cctttgcagc aacggttttt tgcacgacgc catgcgaaag attctgcgga
     6781 ccggggcatc cgggctttga taaaatgagt tcttccatgg gaccgttttc ttggggagag
     6841 gggatttcat gacgtttccg atcagccgtc cgcggcgtct gcggaaatcc gcgtccctgc
     6901 ggcgtttgct gcgggagacg cggatttctc cggcatctct ggtggaaccg tttttcgtct
     6961 gtccggggaa aaacgtccgc caacccattt cggccatgcc ggggatatgc cgtttttccg
     7021 tcgaccatct gttgcgcgag gcgcgggagt cccatcgttt ggggatcccg gccgttctgc
     7081 tcttcggtgt cccgcgggaa aaggatcctc tgggaaaaga ggcctatgcc cgcgacggca
     7141 tcgtgccgac ggcggtgcgg gcgttgaagg acgccctgcc ggacctcacg gtgatcacgg
     7201 acctgtgttt ttgcgaatac accgaccacg ggcactgcgg cgtggtgaag aaatccggcc
     7261 gttcgttcgt tttggacaac gacgccacgc ttgatctcat ggcgcgcacg gcggtggttc
     7321 aggccgacgc cggggccgac ggggtggccc cgtccgggat gatcgacggc ggcgtgcgga
     7381 ccatccgacg cgctttggat gaggacggga aaaaggacac cttcctgttg gcgtacaccg
     7441 ccaaatacgc gtcggcgttc tacggcccct ttcgtgaggc ggccgcctcg gccccgcttt
     7501 tcggggatcg ccgaacctat cagatggatc ccgccaacgc gcgggaggcc ctgcgcgagg
     7561 cggccctcga cgtcaaggag ggggccgaca tcgtcatggt gaagccggcg ctggcttatc
     7621 tggacgtcct gcggctcttg aaagatcgtt tcagcgtgcc ggtcgccgcg tataacgtgt
     7681 cgggggaata cagcatgatc aaggcggcgg ccgccaaagg atggatcgac ggcgcggccg
     7741 cggccctgga ggtgttgacc tccatcaaac gggccggagc ggatgtgata gtcacttacc
     7801 acgcgcgcga agccgcacgc ggaatgaaac catgaaccgg agcgttcttc gttggatgct
     7861 ttgtttggcg atggcggcgc tgccggccgc ctgtgcccgg cgtcaaatgg tcaaaccgcc
     7921 cggaaaccca gagtggggag tgcggatcgt ttggtacggc cactcctgtt tttcttttga
     7981 agacagcgtg gaacggaagt tcctggtcga cccgttcgac gagacggtgg ggtacgacat
     8041 cccctggatg aagtcggacg tccttatggt cacgcacgaa catttcgacc atgatttcat
     8101 ccgccgggcc gagaggtgcg aagtggtgaa caccaccggc gtcatcaccg ccgcgggcgt
     8161 cgagatcgaa gggggcctcg gttttcacga cgccgagggc ggcgcccgtc atggggtgac
     8221 gcggttttac gtctgggaga tggggggcct gcgttttgcc catctgggag acctcggcga
     8281 cccgaacctc tccgaggcac agaaggaacc ccttcggaac gtcgacgtcc ttttcatccc
     8341 cgtgggcgga aagacgaccc tggacggggc ggccgccgcg gcgctggtga aggaacttca
     8401 acctcgcctt gccgtgccga tgcattatgg gaatccgcgc gtccggtttt tcgagtttga
     8461 gccggtggaa tcgttcctca gccattttga tgacgtggtg cctctggcgg acaccgattt
     8521 tcttgtgcgt aaagacgatt tgccggcggg gctgacgatt tacgtgccgg ccctgccgcc
     8581 ggcccggcga tgagggatgt cccggtgtcg ttgattcgaa cgagagcctc ggcgggatgg
     8641 aaagggttct gtgtctaaat ccaaggagct ctatcggcgc gcccaaacgc tgttggtggc
     8701 cggagtcaat tcgcctgtcc gcgccttcca cgccgtcggc ggcgctccca tcttcatggc
     8761 ccgcggccgc ggttccgaga tggtggatgt ggacgggaaa aggtacgtcg attatctggg
     8821 atcgtggggg ccgctgatct tcggacacgc gccgccgcca atcatcgcgg ccgcggaaaa
     8881 ggcgctgcgc ctcggcgcca cgttcggaac gccggtggag agtgaggcgc gcttggcgga
     8941 actggtgcgc gccgcgttcc catccatgga attgatgcgg ttcgtcagtt ccggcacgga
     9001 ggcgtgcatg gcggccttgc gtttggctcg cggctatacc ggccgccgtc tcatcgtcaa
     9061 gttcgcgggt tgctatcacg gccactcgga cgggctcctg gtgtccgccg gcagcggcgc
     9121 cgcgaccttc ggccatccca cctcggcggg cgtcccccgc gagatcgccc ggttgacgct
     9181 ggtcttgccc tacaacgacg ccacccgggt gcgaaaagct tttcgcagcc acggccggga
     9241 catcgccgcc gtcatcgtcg aacccgtggc cggcaatatg ggagtggtcc ccccggaaaa
     9301 acaatttttg cgcgtcttgc gcgaggagac gaaacgtcac ggcgccctcc ttgtcttcga
     9361 tgaagttatc acgggcttcc gtctggcctg gggaggggcc cagacggtct atggggtccg
     9421 gcccgacctc acctgcctcg gcaagatcgt cggcggcggg ttccccgtcg gggctttcgg
     9481 cggggccgcg aaaatcatgc ggcgcctggc gccgctgggg ccggtgtatc aagcgggaac
     9541 gctttccggc aaccccgtgg ccatggcggc gggcatcgcc acattggaac tgttgcggcg
     9601 gacccggccg tacgcctctc tggcccggca gaccgcccgg ttgtcggccg gcatcggggc
     9661 cctggccgcg ccgcggggcc ttgccgtgac cgtcaatcgg gtgggatcca tgttcaccgt
     9721 ctttttcacc gatcgccccg tgcgcgatgg tttttcggcg atgcgttccg atgcgcgacg
     9781 gtatgcccgt tttttccagg gtctcctgaa gcggggggtc tatttcccgc cggcgcaact
     9841 tgaggcggcc ttcgtctctt gcgcccacac cgcggccgac ctggaaaaga cgttgaccgc
     9901 ggtcgccgaa acgttcgagg agctgtgatg gagatattgg ttttgtccga agccgacgtg
     9961 cagtcccttt tttccgttaa ggaagccatt ccgttggtgg aacacgcttt cttggagctg
    10021 gcccgtgggg ccgcccagat gccgcccaag gtgtatctct cctttccgga attccagggg
    10081 gatttgcggg ccatgccggc ctatatgcct tcgaccggcg ccggttcggg aaaaccctat
    10141 gccgcggtga aagtggtcaa ttcccatggg cgcaatccgg agcgtggtct tcccgccgtg
    10201 gccgcgacgt tggtgttgaa cgatccggag acgggactgc cgctggcttt cctcggggcc
    10261 ggccggctca ccgataggcg taccggcgcc gccggggccg tggccgcccg ttgtttggcc
    10321 cggccggaat cggccgtggt cgggctcgtc ggttgcggcc gtcaggccat gacgcagctg
    10381 gaaggactga ggaacgtttt tcagataaaa aaagtgttgg ccgcgggccg gaccttcgaa
    10441 gaggcccggc agttttgttc cggactcaag ggagaagatc tcgaagtcgt tccctgcgag
    10501 gacgttcgac gggcgtgcgc ggccgatatc ctcgtgacca ccaccccgtc ccgttctccc
    10561 gtggttcggg cggaatgggt ccggccggga acccacatca atgccatcgg cgccgacgcg
    10621 cccgggaaac aggaactgga gacggctctt ctgcggtccg cacgcgtggt ggtggattgg
    10681 ccggaacagg cgttccattc cggagaagtc aacgtgccgt tcgccgccgg cgccctgaaa
    10741 aaagaacagg tggtcggcca tctgggggag atcctcctgg gggtcaagaa aggccggacc
    10801 tcgccgtcgg acatcacggt gttcgattcc acggggctgg cggtgcagga cctggccgcg
    10861 gcgtcttggg tatatgaaaa agcattggcc gaaaaaaaag gccgaaaaat cgtaatttga
    10921 gaggaaaata atggcggaga tcaggccgtt tcgcgcgttc catcatcatc cttccgttcg
    10981 gcgggagttg cgtcgtctgg tatgcccccc gtacgacatc atctcgtccg acgaccaaaa
    11041 gcgtcttttg aggaaacacc cggagaattt tgtccggatc gaactgccct ccggtcggcc
    11101 gccgggaaaa tatcgtcggg ccgccgccct gtggcgtcga tgggtcgaac ggggttttat
    11161 catacgggat ttctctcctg ctttttacgc ctacgagacg gagttcgtct cccgtctcgc
    11221 ccgacgtcgc ctccgtcgcc gcgggttttt tgcggcgctg cgccttcggc cctggggaca
    11281 gggcgttcat ccgcacgaaa aaacattgtc cacgcccaag gccgaccgtt tcaaattgtt
    11341 taagtccatg cgcctccaga cgagcccgat ccaatgcctc ttctgggacc ggtcccgttt
    11401 ggcgacccgc ctccttgccc gggtggcgag agggggggcg tggagggaat tttcggatac
    11461 ggaggcgcgg caccgcttct ggcgtatggg ggacgccaag accatccgac aactgcagaa
    11521 ggtcctcgcc gcttccgacg tggtgatcgc cgacgggcat catcgctacg agacctcacg
    11581 cgcctacggc gcttggtgcc ggtctcgggg gcaagcgaca gagtcttccc gttacgtcat
    11641 ggcctacttt tcgccgacgt ccgacgcggg gctggaaata ctccccacgc accgggccgt
    11701 cggcgcggac aaggcgcact tcgtcaatct ggatcaatgg gggaaaatgg aacccgtccg
    11761 ggattacgcg catctccaac gccgcctgcg ttcttcccgc gccgcggcgg tgggggttta
    11821 tcgggagggc aaatattatt tctatcgctt caccgccgtt ccggcttcgt tgcgccgaac
    11881 ccccttcgag aattcgtcgg tcgctttgct gcacacaggc cccctggcgg gactgggaag
    11941 agaggatttc ttcttttccc gccgtgccga agaagccctc cgctgggccc gccggagcag
    12001 gggctggggg ttcattttaa cgcccagcac ggttgaagag gtgatgcgcg tcgccctttt
    12061 cggactggtc atgccgccca agtccaccta tttctatccg aaaatcccct ccggcattct
    12121 gggccggtct ctgagtgggc cgctttgagc tcccgtgttt ttcccgggac gatttccgtg
    12181 agtcgttgac cggccatgtc gggcttggat atcggcatcg tcctgctgac ggtggccggc
    12241 attttctttg gattgaagat cggcgtcatc tccgcctgtt tcaacatcct cgccgggttt
    12301 gcgggcactt gggttgcctc ccgtttctat gtcgtgttgt ccggatacct cccgcgtccg
    12361 gcgttttcct atttcctcct cttcatcatc gtggccgccg cgttggtggc cgccgggatt
    12421 ctgatcagtc aggcgctgga atcctatttc ctcgggctcg tcgataaatt tctgggggcg
    12481 ctcctgggga tggtgttgag cctccttttt tcgtcggtcg tcctttttcc gttggtgttg
    12541 gaacaaaccc ccgccatcaa aaatctcatg cgccgctccg cgttcgcgcc ctacgtgatc
    12601 cggacgacgc agcgatacgg ccgcctcgcc gcccaggacg tctgggcccg cgtggagccg
    12661 ctcctggaat cggacgacct gcgccgcgtt cggaaactgt tggaaaactc cccgcgttga
    12721 cggcttctcc gtgccgctcg tcaaaagaaa aaccggcgtg gagcggcaag tgagcggcga
    12781 ggcgtcggtc gtggcccgca cgctctggga ctttgtgggc gcttccccgt ggcgtttttg
    12841 gctcgaggga gggccggaag gaaccgtcgc catcgcctgg ggccgtcctc tttgggaatt
    12901 ccgagggaaa aacggcgtcg gttttctgcg gaccggccga aaaatccgac gtttctcttt
    12961 ctcggacccg ctgcctgtcc tacgcgaaca actccgtttc gaaaaaacgg atgacgatcg
    13021 gggcgcctcg cttgtcgata tgggcatgcg ctgcggtcga ctcnnnnnnn nnncggggcg
    13081 cctcgtcgtt ctccggtggc gcggtgggat actggggtta cgaatgtctg cgatatttcg
    13141 agccgtcgct gtctcggccg cggaatcgag cacatcgccg gcccggcgga gacgccgttc
    13201 ccgatttcat ttgggtgatg cccccttgct gggccgtggt cggtcccttg gggtcgctca
    13261 cattttttcg gcagacttcg gccggccgga tggcttcatc atcgaaagag cgaaacggac
    13321 gccggccaag cgtgaaaaac cgtttctcgt cgaccccccc gccggatttg aggaaaaatc
    13381 ggggcgctta catggcgggc gtccggcgca tcctggaata catcgccgcc ggggacatct
    13441 atcaggccaa cctttcccat cggctggagt tcccctggcc ggccgggacg gatccctccg
    13501 tgtattttca tgcccttcga cgaatcaacc cttctcccta cgccgctttc ctgggatttc
    13561 ccgatttctc catcgccagt tgttccccgg aactcttgtt gcgggcgcgc ggccggaggg
    13621 tggagacccg tcccatcgcc gggacgcggc cccgcgggca caacccctcg gccgacgagc
    13681 ggctttccgg tgaacttgta ttgaacgcga aagaacgcgc cgagcacatc atgctggtgg
    13741 atttggagag gaacgatctg ggacgggtgt gccatcccgg atcggtccgg gtttccgaac
    13801 agatggtctt ggaaaaatat tcacatgtga tgcacatcgt ttccaatgtc caggggaggc
    13861 tgcgttccgg cgcggatgct ttggacgctc tgcaagcgtt gttccccggc ggcaccatca
    13921 ccggctgtcc caagatccgc gccatggaga tactcgacga gctggaaagg gtgccgcgcg
    13981 gccctttttt tggaagcgcc ggcagaatct ccgcctccgg cgacgtggat ttgaacatcc
    14041 tcatccgcac cgcgctcatt cgccgcggcc ggctcgcctt gcgcgtcggt tcgggcatcg
    14101 tggcggactc ctccccgtcc cgcgaatacg aggaaaccct ccataaggcc cgggcccttg
    14161 tgaacgccta tcggatgtgc aataactctt aaagaatgag gaattttaaa aaaacgatgc
    14221 cgaaaaaaat ctgggtcaac ggacggctgg tcgatccccg ccggcccgcc gtttccgtct
    14281 tcgaccgcgg tttcctctac ggcgacggcg tatacgaaac catccgcgtc tatgacggaa
    14341 aaccgtttca gctcgagccg catttccaac gcctcgcgcg ctctctccgg gggattggaa
    14401 tgcgggtccc tttcggccgg gccgtccttg gagacgccgt cgcgtccgtg atggccgcca
    14461 accggcttgc ggacggagtc gtccgcctca ccgtcacgcg gggcgagggc gccttggggt
    14521 tcgaccccgc ctcctgccgc cggcccacgg tcgtgctcct ggccgtgccg ttcgcttccc
    14581 atccggcggt ccattatgag aagggggtcc gcgcggtttt tgtccgtctt cggcgcaacg
    14641 cgaagaatgc cctggatccg tccataaaat ccttgaacaa cctcaacaat atattggcca
    14701 aaatggaatc catgaaaaaa gggggacagg aagcgctgat gctcaatcag gaggggtatc
    14761 tcacggaagg gactatttcc aatatttttt tcatttccgg cggcgtcctg aagacgccgt
    14821 ctttggattg cggtcttttg gacggggtta cccgatccgt ggtcctttct ctggcaaaac
    14881 gccgttcgct ggccgtgtgg gaggggcgct gtcgaccccg ccaccttttt tcggccgagg
    14941 aggttttttt gaccagcacc acgctggaag tcatgccggt gacgtcggtc gtcgacgagc
    15001 aaggacggct ccgacgcatc ggttccggac aggttgggga atggagcggc cggcttcttc
    15061 gggattaccg tcggctgatt acgaaggcga cggggaaaaa attcaagata tgaaaagacg
    15121 tacgggaatt ctcgtcgccc tttttctgtt gggctccctg gggacggcgg ccgaagaaaa
    15181 ttcgtcgccc caatcacaga agagggcggt gtccgtcgag gcctccccgc ccttatggac
    15241 ggaggacccc gatccggcgc cgcgtcctcg acgcgcccgc ccgcgggagc gttccgccgc
    15301 cccgcggttg ttttggcaag tcgatttttg gacggggaac gacgcttttg aggaggatga
    15361 ggaagccgcc cggcgcttca gcgcctcgct gctctccatc ggctatgacg ccgcccaggg
    15421 atctgtgcag aattccggcg ggttgggatt gcgtttcggc cttcttttcc cgaccatgac
    15481 cggttgggat atcgggggca gcgccggata tgtggcggga ccgactcagg atgtcacggt
    15541 catcgccctg agccgggccg ccggcgacgg aatgttggtg gaggaagcgg aaacggattt
    15601 ctatcgattc ctttttgagg cgcaaaagga agtcccgctg agccgctggg tgggcctccg
    15661 gttgaggggg ggcgccggtt gggccgccgg aaagatgcgc gtccgggacc aatacaccgg
    15721 cacttttgtc tcccttctcg gctacccggc ctcagcggaa tatgtcaacg gatggggcgg
    15781 ctttacctgg gaagtgatgc cttctttcgt gtttcgtgga aactgggcta attttgaagt
    15841 ggggttgggc tgggccggtt ttccgaaaat ggaagaaacc gacgatttga atgagttcgc
    15901 ctggaacccg ctcgggtttc ggctcggttt ggagtttcgc tagccaggtc cggttgtttg
    15961 tagctccgga tccgcctcaa acgcacgccc tttcgatgaa tcgacagata agtcggttcc
    16021 ttgggatccc ctaaaaaaat catttccatc gggaacaaag taactggagt tttgttctat
    16081 tcatactcca gaaccagaag acatcatttt ctggtggggg gatgggcggc ggccggtgct
    16141 tccgggtttc ttcgaggaag ggtgcagttt cgagcgctcg tcgtagtccc cagaaggcgg
    16201 cgactgttca gtttcctggc ggtgaagcgg tgggggagga tctggtcgcc gccttctggg
    16261 agtgttgggg gccttcgtcc ctgccaggaa gttcagcacc ccgcagagtg ccagctcccg
    16321 cggggtgctt ttttgggggc aggggatggg gaataaaaaa acccggctgg aaacggccgg
    16381 gtttttttat tcttgaagaa ttgacctatt ttttcttagc cggggcggag aggttctctt
    16441 tgggaagttt tttgaagcag aggaaccaat cgtgacagta atagtctgcc cccgattttt
    16501 ccacgcggtc cttggcggtt ccgcaggaac ccggcggcgg cacgatcacg tcgtctcccg
    16561 gctgccagtc ggccggggtg gcgatggcgt gcgcgtccga tgtctgcatg gcgacgagga
    16621 gacgtttcag ctcttggaaa ttccggccgt tggagagcgg gtagtagatc agggcgcgga
    16681 ttttggagga ggggtcgatg aagaacaccg cacgcaccgc tttcgtgtcg ctggccgtcg
    16741 gctgaagcat cccgtacttt ttggccacct ccattttgag gtcggcgatg agcgggaaca
    16801 ggacttccac gttctgcagg tttttgtact tcgccttttc tttgatggtt cggagccagg
    16861 cgatgtggct gtagatgctg tcgatcgaga ggccgatcaa ctcgcagttc agcgccttga
    16921 actcgttcga catcgaggcg aaagtcatga attccgtggt gcataccggc gtgaagtcgg
    16981 cggggtggct gaaaaggatg acccatttgc ctttgtagtc ggcggggaag ttgatgggcc
    17041 cctgtgtggt ttcggccgtg aaggcggggg cttcttctcc aatcagcgga atgcgcgtgg
    17101 cgtctcccgc cagggcgagg gccggaaggg tgaggaacaa aaggacagga agtatttttt
    17161 ggaaatgttt catggggagc ctccttggaa tgacggatac gatatccgat tctatctttt
    17221 taaaagcatc ttttggcagg aggcgcattt ttcgatgaac tggtacaatg acggtatgga
    17281 acgaaaagga tcgcgcacgg catggctgcc catcacccgg gacgagcttc cggccctcgg
    17341 ttgggacacg cccgacgtcg tcctggtgac gggcgacgct tacgtggacc acccggcttt
    17401 cggtacggcg gttatcgggc gggtcttgga gcaggcgggt gtgcgcgtgg cggtgattcc
    17461 gcagcccaac tggcgggacg atctgcggga tttcaggaaa ttcggccgcc cccgcctctt
    17521 cttcggcgtc acctccggca acatggattc catgatgaac cattataccg cgcatcgccg
    17581 ccgccgcgcc gacgacgcct atacccccgg gggggcggcg ggcttccgtc cggatcgcgc
    17641 cgtcaccgtg tatacgaaaa tccttaagtc cctctatccc gatgtgcccg tcgttctggg
    17701 aggcattgag gcctccctcc gccggctcac ccattacgac tactggtccg acggcctccg
    17761 gcccagcgtc ctccaggaga gcggcgccga tctcctcgtc cacgggatgg gggaagaggc
    17821 cattcgccgg attgccgaac tgctcatgcg aggcgtcccg ttctcctcgc tgaaaaccat
    17881 ccctcagacg gcgtttctga tggccgccga tgagccgctg ccaaaaaaca gccgctggga
    17941 gacactcgct ttgccgtccc atgaggaatg tgccggcgat aaaaaagagt ttcttaaaag
    18001 ctttaagctc tttgagacgg agtccaacag gctcaacgcc cgccggttga cgcagcggct
    18061 cgccgaccgc cggatcgtgg tgaacccgcc gttccctccg ccgtcggcga aagagctgga
    18121 cgcgatctat gatctgcctt tcacgcgcct gcctcatccc aaatatcaaa agcggggccc
    18181 tgtcccggct ttcgagatga tcaagttttc catcaccctc catcgcggtt gtttcggcgg
    18241 gtgcgctttt tgcaccattt ccgcccatca ggggaaatgg atttccagtc ggtcgaaaga
    18301 atccgtcgtg agggaagcgg gagccgtggc ggacatgccc ggttttgcgg gcgtcatcag
    18361 cgacctgggc ggccccacgg ccaacatgta cgggatgaga gggaaagaca cggcgctttg
    18421 cgcccattgc gcccgatcgt cctgtctgtt cccggccatc tgcgagaatt tgaacacgag
    18481 ccacgcagct cttttggagc tttatcgggc ggtggagggg cattcaaagg taaagaaggc
    18541 gttcgtttcc agcggggtga ggcacgacct ggcgttaaag gacgaatcct atcgggagac
    18601 gttggcggcc cggcatgttt ccggccgtct aaaactcgcg ccggaacaca ccgaggagcg
    18661 tgttctgaaa cttatgcgca agccgtcttt cgaggcgttc ttgaaattta aaaaatggtt
    18721 cgacggattt tgcgcccgcc gtgggttgag acaggagata ctgccgtatt tcatttccag
    18781 ccatccgggg gggacggagg aggacatggc gcggctcgct gtccgtacga aggagttggg
    18841 gtatcgcctg gaacaggtcc aggatttcac gcccaccccc ctgaccctgg cctccgtcat
    18901 ctactacacc ggtttggacc ccgacacgct taagccggtg cctgtggcaa gggcgcccca
    18961 ggaaaagaag gcccaaaaag atttcttttt ctggcacaag ccggagatgc gtgccgtcct
    19021 gaaaagggcc ctcctccgtc tcgggcgggg cgacctgatc cgtcggctct tcggctgaag
    19081 agggaggggg gccgttgttt ttcgtcggcc aaaacgatag acttgttttc ccggtcttcc
    19141 cgacgggatt ccggcatcgc ttttcgaggt tattatggcc cgcgtggacc tgaaaaacgt
    19201 cgcaaaaaaa ttcggcgacc attacgtcgt caaaaacgtc aacctggaga tccccgacaa
    19261 ggaattcttc atcctggtgg ggccttcggg ctgcggtaaa tccaccaccc ttcgcatgat
    19321 cgccggcctc gaggaggcct cggaggggac cgtctccatc aacggccagg tggtgaacga
    19381 cgtcccgccc aaagaccgcg acatcgccat ggtgttccag aactacgcgc tttatccgca
    19441 catgaccgtc tatgagaaca tggcgttcgg gctgaagctt cggcggtatc ccaaaagcga
    19501 gatcgatcgg cgcgttcgcg aggcggcgga gatactgaac ctcggaccgc ttctgcagag
    19561 gaaacccaag gcgctttccg gcggccagcg tcagcgtgtg gcggtgggcc gggccatcgt
    19621 gcgcaagccg cacgtgttcc ttttcgacga gcccctctcc aacctggacg ccaaactgcg
    19681 cgtgcagatg cgggtggaac tttccaagct gcacgaccgc ctgcagacca ccatgatcta
    19741 cgtgacgcac gaccaggtgg aggccatgac catgggcgac cggatctgcg tgatgaaaga
    19801 cggggagatc cagcaggtgg ccccgcccct ggaactctat cacaagccgg ccaaccagtt
    19861 cgtggccggg ttcatcggca atcctcccat gaattttttc ccggtgcgca tcgaaaaaaa
    19921 caaggacggc gtgtggctcc ggcacgaggc cttctccctg gccgcccccg ccgccacggc
    19981 ggacaagatc cggccgcgcg tgggacagga ggcggtcctg ggaattcgac cggaggacat
    20041 ttacgaccgg cttttttatt cctcccaagt gccggaagga agcacggtga aggcggtcgt
    20101 ggaggtgttg gagccgatgg gttcggagat ttatctctat ctcaaggcgg gcaagaccga
    20161 cgtggtggcg cggatgccgc cctacgtgaa aaccaagctc catcaggaga tcgaattggt
    20221 tttcaacctc gagcgactcc accttttcga caaagaaacc ggcgtctcgc ttctctgacc
    20281 ctccgcggaa tgttctggaa ggccctgacc gacgtcgtcg ttttcctgca cggcttctgg
    20341 gtcgtggccg tggtcgtcgg tcctgtgtgg gcctggaaaa acaagagatt tcgcgctgtc
    20401 cacctgggga tgctctggtg gacttttttt gtactggctt cgggtatcta ctgcccggtt
    20461 tcccatctgg agaactcctt ccgcatgcat tacgatcctt ccggctccta tcccggcggt
    20521 tttgtggtcc attatgtgaa ccgcatgacg tcttgggacc tcacccagcc gcaggtggtg
    20581 agcgcgatga ttttttggac cttcctgtgg accgccgtct acgttttttt atggacgaag
    20641 aaggcaaagc cttcccctga atcacgaaaa taaattcgcg ggatgaagat gaaaagtcgc
    20701 ctgttccggg tcgctttctt ttttttcgcg gcggggacaa tatccattcc tccggccggc
    20761 gccgtgggac cggactacag cgacatctat aacctcgacg ccgaatacga gctggaccgg
    20821 ctcatggggg atatcccgtg ggaatggcgc gaccggttcc gaagttccgt ctccgggttt
    20881 caggggacgg gcggttcttt gaacatccgg catctctatt ccgaacaggt tttcaaggcg
    20941 cggtttccgc tgcttcagga ccggctctgg ttccggatct tggacaaaag gaaccgtggg
    21001 ctggagcggg aagaggtgga aacccatctg gaatttgaat acagtccggc gccccggtgg
    21061 tatctgtcgt ttctgggcgc gccggcgctt cataagtcgg atgtggtggt gggggcggcg
    21121 gcccgttggg ggagggacga ggggcgctcg gtccgattcg cctatctgtg gccggggttc
    21181 gacaccaatt atgctttcca caaccgctcc atcaacgaag gctacgagga gttttaccgg
    21241 cgttttccgc aagaagcgcg tttgtccgct gtttggatac ggtccccgct cagttttttc
    21301 atcgaggggc gccatcggcg gacctcggaa ttggagcatc aggaattctc gccgccccgg
    21361 acgcgcttta tttccggaga cggtgaggcg gaggcggtct gggacgtgcg ttacgtcgcc
    21421 ggccgttggg cctgggggac cgaagggagt ctttggcggg cccgcgaatc cctgcgctac
    21481 gaacccgccg cggcggcgct ggatcaaacg ctcatccggg aacggtccgc cgctcgattg
    21541 acgcttcggc ggacgctcgg cgccggatgg gccgtgcgcg tcggcggcgg gatggtgggg
    21601 tcgcgcgggc atctccggta tccgggccag ccgttgtcgg accgcgccta ccggatttat
    21661 gaccgcatcg gctatgtggc ggccgatcat gccgtcacgg cgcgctttgg ggtggaggcg
    21721 ggatattttt atgaccgaca ggggagaaaa agcgtggaaa cggggaatgt agccatcatg
    21781 gaaaaaaact cgcaaaaccg cgggaaattg gcgatacgat acgatttctc cgaaaacgcg
    21841 tccctgcggg tgatttccgc catcgagttg gatcagaatc aatcggagaa tttcgcgtcg
    21901 ttcgacggag ggaccatcca attccaaacg actttctagc gaatatcgcc gccaaggcaa
    21961 aagcggccgg aatattcacg gggatcttct tgtgcgggac gcttctgtcc tccgcgccgt
    22021 cgcaaaaaca gggacatcgg cggtatcggg aagccccggc gtcggagttg tcgtcggcgg
    22081 gcaattaccg gtcgacgggc cgcgtggtga aacttcgctc ggccgcggcg gaatctttcc
    22141 gggccatggt cgccgccgcc tccgccgagg gggtccggct catccccatt tcggggttcc
    22201 ggaccgtcgc ctaccagaaa gttcttttcc gccgtgcctg tcgacggtat ggatccgaaa
    22261 aaaaagcggc gagatgggtg gcccctccgg ggcattccga acaccatacc ggatggtccc
    22321 tggatctcgg ggaggagtcg tcgccggaga cggacgtgga cgcgtctttt gaaaacacgc
    22381 gggcgttccg atggctggag gaaaacgcgt cccgattcgg attcgagttg tctttctcga
    22441 aagacaaccc gcaaggcatc ggttacgagc catggcattg gcgctacgtc ggcgcggagg
    22501 cccccaaaaa acttttttcg acgccgtagg ccgacgaggc gtttccggcg gcaagggctt
    22561 tttgcccgca tccgatgaaa acagtcgagg gaaaacattt ttcccccggc tgtttttgtt
    22621 ttgaggcgct cgacgggacg aagtcccgtc agccgatggc cacagaaccc ctttcgccgg
    22681 tgcggatgcg aacgcattcg gaaagatcga gaacgaagat cttcccatct ccgatgtgtc
    22741 cggtgcgcgc gcccttcacg atggcgtcca tggccggttc cacgaaggcg tcgtttaccg
    22801 cgatgtcgag acggaccttc ctcagcaggc tgccagcctc cttgtggctt cggtagacct
    22861 cggaaatacc cttctggcgt cctctgccga gcaccgtggt ggcggtgacg aggtggatct
    22921 cgttttcctc cagccgtttg agtacttcgt ccacccggtc gggttgtatg atagcaacga
    22981 tatatttcat ggcaatctcc ttaatccaat agagtgtagg cggcttctct gtggtcggtc
    23041 aggtcgaggc cgatccgttc gtggtctgct tccgctctta cgccgaccag cgccttgacg
    23101 gccttcaaca ggacgaaagt gacgacgaag gaatagaccg ccgtcaccag cgtggctttt
    23161 aactgaatga gcaactgcag gggattgccg tagaacagtc catcggctcc ggccggattc
    23221 accatctttg tggcgaagag gccggtggcc acggcgcccc acatgccacc gatgccgtgc
    23281 acgccgaagg catcgagaga atcgtcatat cccagccgcg gcttgatcac cgccaccgcc
    23341 acgtagcaga tcaatgaaac accgagaccg atcatcagcg cgccggcaac attcacgaac
    23401 cccgccgcgg gcgtaatggc caccagccct ccgacggctc ccgtgattgt gcccaggacg
    23461 gtgggtttgg agttgaaaac ccagtccagg agcgcccagg tcagtccggc ggcggctgcg
    23521 gcgatgtgcg tcaccacgaa tgc
//
LOCUS       DAOTFD010000020        23373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1442_length_23373_cov_4.411013, whole genome shotgun sequence.
ACCESSION   DAOTFD010000020 DAOTFD010000000
VERSION     DAOTFD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 23373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23373
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_23373_cov_4.411013"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1334..6244
                     /locus_tag="P5079_02470"
     CDS             1334..6244
                     /locus_tag="P5079_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28884.1"
                     /translation="MNFLEFSAGGYLFSTEKMTCQYDASSHLVKSNVETFQVGRQEQG
                     GAGMFLNRRFNTETEYLKYSAAGQVLRQKTTQCEAGGCAVTESLSDNLYDRQGRLVRS
                     HTRTTSEAGEVSETEWQALKMDAAGRVLRHESVIRSADGTTVRQWSLEDSRCDGAGRL
                     VYEKTAVEETGTDGVVRGSMKEQWFLGYNAFGQVVRRETVLHRPGPEGDVRRTKETEE
                     FRYDARGRTARTTITGVENIGGRERTYSSILEIFKFDDKGRACRTRSVQERDGVRSTR
                     TSLNDIVYDDPGRVIESRDRVETSGLGLAKIVVESYADIVYDAWGRRLQYTRATEDGA
                     LSVVEKVERVDYDAWDRVTAMLSLTTEFSRDPAAPLFRQTHTSLVGMRYDRRDDLVGY
                     EKTMQDGDGVTHFHPVSLQYDEQGRLVESLVRVVSDDGRDFFEWSYGLAYNALGQMTD
                     GWQGTLEMRDGGVPGENVLERMKSWGSVHVGLFGRSRGAVMSARERDDGVKNVAMTRL
                     SNLRYDAQGRLIHSESVRYSMTRNVKGVVIEEHLTRGRTEQRAFDSLGRAVRSIEETW
                     QNGEYRTVVAYVGYDIQGRQTHRRDFVSISGVNGDGSRLQRSYVQEQSFEFGALGRPQ
                     TITTRLRHDTAAPLKDETTVVSGIVYDAVGRRIAWTETSVSGGAPEKTLILNVSNAQY
                     DNAGRLVGADQTKYEEAGGNRRLLYADTGVVNAYDIRGRFVESTAERSWNESGSGGGE
                     VAGLDDGWATGRVRVKTVYVYAGVGDSLISSRTEAHGTGAHSNALLTPGGIQLMYEQK
                     DIKYDESGRMTTYRQIVTQRESYTFQKEVQKGLRKKKKSGLRLESVTTDTYVTDIQYD
                     GFGRQGHSVSVSQRNDVGRTQTTTVSTVKSFDGLGRAKDVERVTDSRFTLPKKRGGFF
                     QRLGTAVIPGGGLIGGTVLALRKGDLVGGKEVQSYSHVTQTLAYSANGTVDDVRTQTI
                     TLKEFVKDKGKNWGDRVLQAGDIVVAVGATVATVATAGAASPLAAMAWAVAIGGTQMA
                     YQGARQGVAINDFEAHADREQAHASRMMAANFAVTVFMVGVGDNVAASTTFGTTAGTL
                     NTTGRIVYAGMKLVLSAGVAVAGGADRKTILKVVAISTVSGMLESLMVSAGDGRGNVW
                     LSGGGELVRQIGMTYGDEKQRSMWMILGSAMQSSAGGSTSAAWSILRIALIDAFARGR
                     ATQDGEELEMRRRGMVAQAWSELVGNMVSMGQMYWSAKAARISSGLTKGGAAEVVKSE
                     AAESLFEQLKPFFERETEVSLPRGLRPLKVHGYIVGEVYASAKASLWNMMTETVTDAV
                     EDGTEWTSAVTKNAGENVVTAVTRTTDKATEVAGAVKGKAVIAAKSTKTTSKEVYKYV
                     EQKTLKVGHKAWEIKCDAAGKAISILDGDAYDQQPAVIFPSDPDMLALISGNGMLNSM
                     DDAIAMSKLVGTSFWGEGGTSAFIRNDHGWLIGDIIQSIGHEVLGAKDKPAQEMLKAI
                     RLGIAEKGIVFVVCHSQGSAYFHQAIRHLEPEERKRVHYLGLGPEWIVDVEKEGLGSA
                     LNVVNKDDIVPRIMQNGAMNAINRAIPDRRGRMQTEELIRRGNLIINRKPDGVGSPHY
                     LRNYVGAINEWAKDMREIWDDYRRTK"
     gene            6241..6723
                     /locus_tag="P5079_02475"
     CDS             6241..6723
                     /locus_tag="P5079_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28885.1"
                     /translation="MMKRFVKLIMLFVFATGCGRVDRDLSNDSHYMGVIGKQYRTKIE
                     LLVYRFKGNKFETIGESGGGIELPPINETKGKEFPYRYYDTYVFGVLPAGSEFRVAMV
                     REEGAYNNAFIYYYVEITKSDDRQWVGKTVNVSSRIIASDDPLTFKSGITEEMSFDNN
                     "
     gene            6853..7023
                     /locus_tag="P5079_02480"
     CDS             6853..7023
                     /locus_tag="P5079_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HRY28886.1"
                     /translation="MFKWLRRVVWGWLMPFFARHPFLFLLAMGLYVVSPFDIVPEALT
                     GPFGFADDVMLF"
     gene            7163..9352
                     /locus_tag="P5079_02485"
     CDS             7163..9352
                     /locus_tag="P5079_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28887.1"
                     /translation="MLEADGTSTRRGNLSEHFYVLAAGVLLATIAILDLDFILRFAGW
                     EARMNVIDTAKWVEWVRPGFFSKNYSKFIFNFSLIVLPSFSILLAHFFAKIRTKKITL
                     FLLSSAGFFVFMRWGYLKEGLGWKQLGIVNGIILVAYLLGLIVDRWLPRLGVSREPFS
                     VLEFVSEGMTGVGAGKVRSFFNKYQLFLVVPLLFVVLINFAYKYSAGIWINYYHQNFL
                     IAPINDMLHGKYFLVETFSQYGMFYPITLFLVFYVKLLKFTYMNFYLIAMVFTVGYFL
                     VLYYFIRSVTRGLVFAILALFVAMGVNTLFNNAYFPYSEIYDWPGAMPLRFFLDGLVF
                     WLIVRNNGFKNKILSGLVPCLVAAALYYNFETGVCLAAAYGALCLLHVLNGREGGAKA
                     AAFLAKQVGSVSVGAVAIYLGIVFFTWVRIGKMPELDLLLGIPLMMARDGYTALPTPK
                     IGWYWAYLAVYFSVFVGVFYCVLKTRQTSWKWVVLGAYAVYGLFLLQYYMNKSVSSNL
                     PVVGIPFAVLVVVVTEEVLGFLCRHKKADWPLFLVVIAFVGFLASGVLAARTSCYLVK
                     KINYRIYSKGQLKLLKKYENNNGLIVSYSLPPGLMAEDLLASVARVRELTKGQERIFL
                     ISRHDTVHLVMAEKTNYLPCPMLEQIFLKEQLAELKRNLINETKRPLYLFTDRGFPLS
                     PITAPSSPNAYDVLVELFQGVEPYYELIEQVGVINIYRLKKSLGAGV"
     gene            9349..9897
                     /locus_tag="P5079_02490"
     CDS             9349..9897
                     /locus_tag="P5079_02490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY28888.1"
                     /translation="MKPRRLGRRVVYTHPWLDLYVDRVRFPKGRLVDHHVVYFKRESV
                     ATLVENAEGKILLIRSYRYITDSIEWELPAGGFEKGETVFEASRREVREETGYDTSGH
                     RLLYTYNPVNGLSNKVFHVVHCRAGKIVGKFDGNEVKGVRWHTQKQVREMIRRNRIHD
                     GFALASLLLYFFGVPAKEAKNS"
     gene            9995..10978
                     /locus_tag="P5079_02495"
     CDS             9995..10978
                     /locus_tag="P5079_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRY28889.1"
                     /translation="MTDSPAAVSLAHYLRDLLKNAVRDTTARMVLFSGGLDTAVLAHL
                     ASRLRGLGAITVTVGGNPPDKEFAVRAAKHFGITHVLVSPTHDDLLGLLPQTIKLLKT
                     YDPMTLRNTLPVHTALLMAKEMNVRTVLSGDGADELFAGYSFFFDKPLEELPDALRRL
                     WKVMSFSGIELARRLGMEVQAPYLSPMVREFAEKVPPGMLLAERDGKIFGKAILRQAF
                     EGEIPEDILWREKCPAEFGSGTSGLTKALEQKISDEECERQQGTFWNVDSVRIRNKEQ
                     LYYYRLYRAQFGPPKGVKASAGQGRSCPDCGTPVAHKESDFCLLCGAWPVP"
     gene            11005..12927
                     /locus_tag="P5079_02500"
     CDS             11005..12927
                     /locus_tag="P5079_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY28890.1"
                     /translation="MSGAAGKRIFSGLAALWLGAAPLWAADSVFISVTRTDESMDRLP
                     SNISVVTKEEIAQSGAKTLAEVLETQPAMDVNKTGSLGTFSTLRMRGVPASSQVQVLV
                     DDRPLGGTSLQFIDLSQIPVENIERIEIVRGASSVLYGANTIGGVVHVITKRHTASKT
                     ESYVGVEGGSFRTQVYRAGLGMNKRGFDANVDASRYWTDGFQKNADADNIGVSGNGGY
                     TFKNGARIALDAGHSNHEVGDPQGTLVSPSEWDGSRERDAANTKARTEQDINSGRLQL
                     ALPLGAVGHLQSIFYGSDQDYRTFPDRWTEKADFDQNNRVVGNDTRFFLPVGFLAGAS
                     YQRDRQESESVYSARTAHHVVDWAVYAQQRLTVGRLDLIPAARWDQNGNFGNIVNPRF
                     TAVFRVTDGWDLSANAARSFRAPSFLELFYEGTSFTGNKDLRPETGWTYDVGVRYRRP
                     ESAVVRVTGFYTRIRERIAATSKTYENLPQAELAGAEFEAEGRFGPFTKRVNYTYQRA
                     VGNSAGSTRFKPLTLTPRHAANLQLIWAMKRGWSLANGVRFVDRQFQNATDFVGGVSS
                     PRHIVLPSYTLWNVRLTKRLLGAELFFAVENITNKHYAEAVGSYSDPVTFDSTSTLFP
                     QPGRTLRGGIVIRFLD"
     gene            complement(13003..13950)
                     /locus_tag="P5079_02505"
     CDS             complement(13003..13950)
                     /locus_tag="P5079_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28891.1"
                     /translation="MVRTRSRFFKLLFVPTALLAAAALLAADGKHQKFLSQKQEIFVL
                     AGERVRSAHYDFQRTLKMGEDRLRELHENYKVSGSTPPTALDQDVMDLRTATQQVRLR
                     RARGRVVPENPEAAGLVKRYWEEYKSGYEGLQKDILVHRRVGTPESQVYWDIVKRYDD
                     LRSQTEKNLIYNTPPGRASLDEVVQKDRWQILKAVESPNVKTRNKVIGVVAPEEHPRQ
                     VNVDVAPFYHSQGYLPTISPDRIGAYSIPKGHRSVTQREAGLKIILRVSKGKGVAYIG
                     SSEKELNYGYLLIPPDTAYYIENVGDDNLDIEYIVLSSS"
     gene            14167..15528
                     /locus_tag="P5079_02510"
     CDS             14167..15528
                     /locus_tag="P5079_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28892.1"
                     /translation="MRRVLLGAVCAVGLAGGGTPVRGESPVSLAEAVEKVLGRSPGLA
                     SASAQARERRWAGREAKRLRWPSVRLQSAVTRGDGPVYVFGSLLQQKSFGAENFALDF
                     LNNPPDRTNVRSAVEVGLPVFAGHELSDGERLAGLGMAQAESRLASAVQRTRFETVDL
                     GLKILALREKIRRLDERLADVVEDIESARRLKEKGLVLGSDYYAAEAIQSGLSAWRVR
                     WEKELRAMEKSLNVLAGGETWLAGALNEEFVELLPDGGWEETALKNREELRQAELGRE
                     MAEVSLRRAGRSILPRVEAFASVESNTRNFENNPTDRMVGLRATMALGDPAYSARRRR
                     AAAHAEAAAAQRNETEERVSLEALQQWESLQGAVESLPLLKGLRERARKSLEMFRPLY
                     REGRQSIIEVLRAEEALAMAESSYVDALYQIHSGQARLLLAAGRLDGAAVAALDRRLE
                     ARP"
     gene            15525..18722
                     /locus_tag="P5079_02515"
     CDS             15525..18722
                     /locus_tag="P5079_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY28893.1"
                     /translation="MSESHVSLGLAGRLAQAFIRTRLTPLIVVSSLLMGVFAVVRLPR
                     EEEPQINVPMFDVFVPFPGAGVREVEERIVRVGERKLWEIPGVEYIYSTAEPGHALFI
                     VRFKVGTDPEQAMTRIYTKTFANVDILPPGAGQPLIKPRSIDDVPILAVSLWGREVSP
                     RALRRAAAVLRQEISAVPNVSEIEITGGRRRQFLVHFDPEKLASRRLTPLELAGTLRA
                     SNLRLPAGHYNQNGNAVAVETDAFIRTLDDLKRVVVGVSGGRPVLLEEVARVSDGPDE
                     DEAEVFLAPVRVGGVGGDETFPAVTLAISKRRGANATHVAEEVLRRLERARSTVLPEK
                     IETVVTRNYGDTAKEKSDELLFHMLLATLSVTLLIALALGPREAVVVLVAIPVTLALT
                     LLFYYLAGYTLNRITLFALIFSIGILVDDAIVVVENIHRHYCMNDGRPLWKLAVEAVA
                     EVGNPTLLATWTVIAAILPMAFVGGLMGPYMRPIPVGASLAMLFSLAIAFIVSPWAFA
                     GLLKLWKPRVPSSGEKEGRLDKLYRRSMRAVLESSTKRNLYFGLMALLLAGSVFLLFS
                     KKVTVKMLPFDNKNEFEVVLTMPEGTSLSKTRAAAGEMAEVLRDIPEAKQVVGYVGTS
                     APYNFNGLVRHYFLRRRPHQADLVVSLTGRKERKRQSHAIAASVRGPLREIARKYGAR
                     VQVAEVPPGPPVLSTLVLEIYGPDASLRKRLAALVRELLENTEGVVDVDTYVPDTQPL
                     VRLMVDREKTTLNGIPAALVAQTVGAALQGETVDLAHVEGEKEPVEIRLRLPEERRRS
                     LAAAGAIALLTRNGTSVSIKNLTKPRIVEVDAPVYHKNLQAVDYVIAEVAGEQESPVY
                     AILKLWPEIDKLAAQHGVGLKQYFARSPVNSSENALKWDGEWHITYEVFRDLGIAFAL
                     VLLLIYVLVVAWFDSFTVPLVIMVPIPLTLVGILPAHWAMGAFFTATSMIGFIAGAGI
                     VVRNSIILVDFIQLRLREGMPLKEAVIDAGAVRFRPMLLTAAAVVAGAGVILFDPIFQ
                     GLAVALMAGEIASTLLSRTAVPVLYYLLRRRAHER"
     gene            18712..18918
                     /locus_tag="P5079_02520"
     CDS             18712..18918
                     /locus_tag="P5079_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRY28894.1"
                     /translation="MSVEHRIRLIAGTFILLSLGGAFWLDANFIWFAAFVAVNLIQSA
                     FSKWCLMEDLLRAWDRRRTGKTDF"
     gene            complement(18915..19130)
                     /locus_tag="P5079_02525"
     CDS             complement(18915..19130)
                     /locus_tag="P5079_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28895.1"
                     /translation="MNRHKALHILNPVLGLLFANQALTGLFHRALSAETFEVLHEGAG
                     LVLIAAVAGHVALNWGWIKSGFKRTDA"
     gene            19364..21532
                     /locus_tag="P5079_02530"
     CDS             19364..21532
                     /locus_tag="P5079_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY28896.1"
                     /translation="MELFLKNLILPTALFDNLRLIIVILDEDGFIRYVNPFFCESTGY
                     ARKEVDGQSWFEKFLPDENIETVMGVFRDVLAKEEYVHFDNTIIRKDGTKMFVSWTNS
                     VLREKDGKSRGTLSLGMDISGHKEMEDELRRSEELYRALAESTLDMVYLVDFDGIIRY
                     VNPLASAQFGVPAEKIIGKRLAEVFPPHLAQRHLGVIRKIFETGEVYSGELLEKFPGG
                     EVWIDARLSPVRNQEGKVVSVLGASLNITERKRVESSLKERDELLTGAQQIANLGSYT
                     LDIADSRWTSSEILDNILGIGDDFPRDREGWNLLVHPEDREWLAEYLNSHVLKKKNAF
                     NAQYRIVRYSDRQVRWVYGIGKLVLDEKGEVTRLLGTIQDVTEQKKVEASLRNSEQRY
                     RTTLDSMGDMIHVVDRDLRILLANRAFAEFNRAHGLNSDMLGRTVFEVFPFLPAVVAD
                     EYKKVFESGRPLSTEETHRIDGREYNTETLKTPIFEGEKVTQVLTVIRDVTERKQAQK
                     NQELMQKEILNALTKQQIMIGQTLHDSLGQLLTGVAYMVKGLEEDQKAGRPIYAEETA
                     RIVEYINQGIGQTKSLARMLYPAELERGGLPAALENLANQTRGIYNVGCSVRVEGDFQ
                     VRSDVLCTHLYRIAQEAVHNALSHGQATDIHIALAVRGAEGELSVADNGKGFDEKEAG
                     EGKGLGLKILQYRAQEIGGSVFVRRNSPRGMIVGCQFKVE"
     gene            21538..22215
                     /locus_tag="P5079_02535"
     CDS             21538..22215
                     /locus_tag="P5079_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY28897.1"
                     /translation="MAHEHVKSVSKKKIFVLDDHPIVREGMGQLINRQPDLVFCGGAA
                     SVAEAVRSTAQLKPDMVIVDLSLPKESGMDFIKELRSAHPRVGILVVSMYDEFIYAER
                     VLKAGAMGYLMKQEATDRLIGAIRTVLQGKIYLSERMRSEIVTRTVGGGGESVSSDPI
                     AKLSDRELEIFRYIGQKMSTRQIAEKLFLSVKTVEAHRENIKHKLGLYSAADLFEKAL
                     LWTKMEK"
     gene            23122..23195
                     /locus_tag="P5079_02540"
     tRNA            23122..23195
                     /locus_tag="P5079_02540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23156..23158,aa:Arg,seq:tct)
BASE COUNT         4724 a         5761 c         8017 g         4871 t
ORIGIN      
        1 cgaagaaaaa gaacgggtca cggaggccgt tcagtcttat ctggccgatc cgcgacaccg
       61 gctatccatc ggcctcgcgt tgggacgtta taacgaacag atgagtcggc tccggaccgt
      121 cttgaaccag gcgcaagcca cgttggaggt gttgggcggc ggtgttcagc ggtgcgagga
      181 ggaggtccag gcggcgtctg ttgaatatca atcggctctg gcggctcggg atgcggccaa
      241 aagcagggaa gccgaagttc gggtcctgtt cgaaacaaaa aaggcggacc tggaaacgaa
      301 aagacagagg gcgctcgaag aggcgtatgc gcagatcgag gcgaccggcc gggaaatcgc
      361 gctccgattg agccaacgtg cgttcgtcct ccataatacc gtcgtcacgc tggaagtttc
      421 gcaggccgag gatttgttga acggcggggc cctggaactg atgcccgggg agatgttcac
      481 cctgaatgat ttttcgaaga aagcccgcat cctccagtcc ctttcgcggg aggagaactt
      541 cgtccaaaag agcgtggggc cgaccggccg gaaattttca atgacatggc aaggccgggt
      601 gcggtggggg aacgaagagt tctcttccgg aggtcctttt tcattgtcgt cgatggacgc
      661 cctcggaaat tcaagcgggt cgggggggat ggaaagtctc tcgttggcgg gagacggccg
      721 cgtgacggtt gtcgaggtgg aacgtcaggc aacggattcc ctggggcgtg tcatatccca
      781 gaccacgcgg aaccgggagg tccggcggct ggcgggggag acctccacca ggaactacac
      841 cgtctccctg cagaaccagc gatacgacga acgcggacag gtggcgggtt atcttaagac
      901 aacgcgggag gagccctcgg tcggaggtcc cgcgggggag caaacgcgga tcgaggagtt
      961 gctcaaggca gaatacgacg ataacaatcg tcttgttcac agcgagacgc ggtgtgtcga
     1021 agaaaaaggc gaggaaaagt gggaaagttt tgtgtccgtt tggaacgaca aattcgattc
     1081 ggccggccgg gcggtgttga cgcggaagaa agtccaggag ggtgaacggg tctcgctgga
     1141 gaagtcgtcc gccgaaaacc gatatgacgc cgaagggcgg ctcatcttct cgcaggcccg
     1201 tctttgcgga acgaccgtcg gagagtgggg cggggcccag ggggattggg aacgcctgct
     1261 ggccgcctcg ccgcgggaat ccgtttggaa cgagacgttc gacgttcgtg gaaaccctgt
     1321 ccgcattcaa aaaatgaatt ttctggagtt ttcggccggg ggctacctgt tttcaacgga
     1381 aaaaatgacg tgtcaatacg atgcttcctc ccatctggtg aaaagtaacg tcgagacgtt
     1441 tcaggtcggc cgccaggaac agggcggcgc ggggatgttc ctgaaccgcc ggtttaacac
     1501 cgagaccgaa taccttaaat actccgccgc cggacaggtt ttgcggcaga agacgaccca
     1561 atgcgaggcc ggggggtgtg ccgtgacgga atccctgtcg gacaacctgt acgatcggca
     1621 gggccgtctg gtccgttccc atacgcggac gacgtctgag gcgggggaag tttccgaaac
     1681 ggaatggcag gcgctgaaga tggatgcggc cgggcgcgtg ctgcggcatg aaagcgtgat
     1741 ccgctccgcc gacggaacga cggtgcggca atggtcgctt gaagacagcc gctgcgacgg
     1801 cgccggccgg cttgtttatg agaagaccgc cgtggaagag acggggactg acggggtcgt
     1861 gcgtggcagt atgaaggagc agtggtttct gggatacaac gcgttcgggc aggtggtccg
     1921 gcgggaaaca gtgctccatc gcccgggtcc ggagggcgat gtccgccgca caaaagagac
     1981 ggaagagttc cgatacgatg cccggggccg gaccgcgcga acgacgatta ccggcgtgga
     2041 aaacatcggc ggtcgggaaa ggacctattc gtctattttg gagatcttta aatttgacga
     2101 caagggccgc gcctgccgga cgcgttccgt tcaggagcga gacggtgttc gaagcacacg
     2161 gacctcgttg aacgacattg tctacgacga cccgggacgg gtgattgaaa gccgtgaccg
     2221 ggtggaaacg agcggcctcg gtcttgcaaa aatcgtggtc gagtcttatg cggacatcgt
     2281 ttatgacgcc tggggccgcc ggctccaata cacgcgcgcc accgaggacg gggccctttc
     2341 cgtcgttgaa aaggtcgagc gggtcgatta cgacgcctgg gaccgcgtca cggccatgct
     2401 ttcgctgacg acggaattct cgcgcgaccc ggccgccccg ctcttccgcc agacccacac
     2461 gtccctcgtc ggcatgcggt acgaccgccg cgatgacctg gtcgggtacg aaaaaaccat
     2521 gcaagacggc gatggggtca cccattttca tccggtctct ctccaatacg acgaacaggg
     2581 ccgtcttgtg gaatcgctgg ttcgcgtggt ttccgatgac gggcgggact tttttgaatg
     2641 gagttatggg ttggcctaca acgccctggg ccaaatgacg gacggctggc agggaacgct
     2701 ggaaatgcgg gacggggggg tccccggcga aaatgtcttg gagaggatga aaagctgggg
     2761 ctccgtccat gtcggccttt ttggccggtc gaggggggcc gttatgtccg cgcgggaaag
     2821 ggacgacggg gtcaaaaacg tcgccatgac ccgattgtcg aatcttcgtt acgatgcgca
     2881 ggggcggctg atccattccg agtccgtccg ttattcgatg acacggaacg tgaaaggcgt
     2941 cgttattgag gagcacctca cccgtggacg aacggaacag cgggcttttg attccttagg
     3001 ccgcgcggtc cgttcgatcg aggagacctg gcagaacggg gaatatcgga ccgtcgtcgc
     3061 ctacgtcggt tatgacatcc aggggcggca gactcatcgg cgggattttg tgtcgatttc
     3121 gggcgttaac ggggacggaa gccggctcca gagaagctat gttcaagaac agagttttga
     3181 attcggcgcg ctgggccgcc cgcaaaccat taccacccgt ctccgtcatg acaccgcggc
     3241 ccctctgaaa gacgagacca ccgtcgtttc aggcatcgtt tacgacgccg tcggccgccg
     3301 gatcgcctgg acggagacga gcgtctccgg cggcgcgccg gagaaaacac ttattttgaa
     3361 cgtctccaac gcccaatacg acaacgccgg ccggcttgtc ggcgcggatc aaaccaaata
     3421 cgaagaggcg ggggggaatc gtcgacttct ctatgcggac accggagtgg tgaacgctta
     3481 cgacatccga ggacggtttg tggagagcac cgcggaaaga agctggaacg aatccggttc
     3541 gggtggcggc gaggtggccg gtctcgatga cgggtgggcg accggtcgtg tccgcgtgaa
     3601 aacggtatat gtttatgccg gcgtcgggga ttcgctcatt tccagccgca cggaggcgca
     3661 cgggacggga gcccacagca acgcactgtt gacgccgggc ggcattcaat tgatgtacga
     3721 acaaaaagac atcaaatatg acgagtcggg gcggatgacg acctatcggc agatcgtcac
     3781 tcagcgggaa agctacacct ttcaaaaaga agtgcagaag gggctccgaa aaaagaaaaa
     3841 aagcggcctg cgcctggaaa gcgtcaccac cgatacctac gtcacggaca tccaatacga
     3901 cgggttcggt cggcaggggc acagcgtttc cgtgagccag cggaacgacg ttggccgcac
     3961 ccagaccacc accgtcagca cggtgaagag tttcgatggc ctgggtcggg ccaaagacgt
     4021 ggaacgcgtg acggacagcc gtttcacctt gcccaaaaaa cggggagggt tttttcagcg
     4081 gcttgggacg gccgtcatcc ccggaggagg gctgatcggc ggcaccgtgt tggctttgag
     4141 aaaaggcgac ctggtgggcg gcaaggaggt gcagagttac agtcatgtga cccaaaccct
     4201 ggcctacagc gccaacggaa ccgtggacga cgtccggacg cagacgatca cgttaaaaga
     4261 gttcgtcaaa gacaaaggaa aaaactgggg cgacagggtt ctgcaggcgg gagacattgt
     4321 ggtggcggtg ggggcgacgg tggcgaccgt tgcgacggcc ggagcggcct cgcctttggc
     4381 cgccatggct tgggctgtcg ccatcggcgg gacgcagatg gcgtatcaag gggcgaggca
     4441 gggagtggca atcaacgatt ttgaagccca tgcggaccga gaacaggcgc acgccagccg
     4501 aatgatggcg gccaatttcg cggtaacggt attcatggtc ggagtggggg acaatgtggc
     4561 cgcgtcgacg acttttggga cgacggcggg aactctgaat accacgggtc gtatcgttta
     4621 cgcggggatg aagctggtgt tgagtgcggg tgtggcggtg gcgggcggcg cggatcgaaa
     4681 aaccatttta aaggtggttg cgatatcgac ggtgagcgga atgttggaaa gtctgatggt
     4741 ttctgcgggg gacgggcgtg gcaatgtgtg gctcagtggc ggcggagaac ttgtgaggca
     4801 aatcgggatg acctacggcg acgaaaagca gcgcagcatg tggatgattt taggcagcgc
     4861 gatgcagagt tcagctggcg gatctacgtc ggcggcgtgg tcgatcctta gaatagcgct
     4921 cattgatgct tttgccaggg ggcgtgcgac tcaagatgga gaggaattgg aaatgcgccg
     4981 ccgcgggatg gtggcgcagg cttggtcgga gttggtgggg aatatggtgt ccatggggca
     5041 aatgtattgg tcggcaaagg ctgcaagaat ctcttctggg ctgacgaaag gcggtgcggc
     5101 ggaagttgta aaatcagagg cggcggaatc gctctttgaa caattgaaac cattttttga
     5161 aagagagacg gaagtgtccc tgccgcgtgg tcttcggccc ttaaaggtgc acggctatat
     5221 tgttggagaa gtttacgcgt ccgcgaaggc ttccttgtgg aacatgatga cagagaccgt
     5281 aacggatgct gtcgaagatg ggaccgaatg gacctccgcc gtcacaaaaa acgctgggga
     5341 aaatgttgtc acagcggtca caaggaccac ggataaagca acggaggtgg ctggcgctgt
     5401 aaagggaaaa gcggttattg cagccaagtc cacgaagacc accagcaagg aagtttacaa
     5461 atatgtcgaa cagaagacat tgaaagtcgg gcacaaggcg tgggaaatca aatgtgatgc
     5521 ggctgggaag gcgatcagta tattggatgg agatgcatac gatcaacagc cggcagtcat
     5581 tttcccgtcc gatccagaca tgctagcgct tatttcagga aacggcatgc tgaactctat
     5641 ggatgatgct attgcaatga gtaaattggt gggaacctca ttttggggcg agggtggaac
     5701 atcagcgttt attcgcaacg atcatgggtg gctaatcggc gatattattc agtctatcgg
     5761 acatgaagtc cttggcgcaa aagataaacc ggcgcaagaa atgttgaagg cgattcgctt
     5821 ggggattgcc gaaaaaggca ttgtttttgt ggtttgccat agtcaggggt cggcatattt
     5881 tcatcaggcc atccggcatt tggagccaga agaacgtaaa cgagttcatt accttgggtt
     5941 agggccggaa tggattgtag atgtcgaaaa agaagggctt ggaagcgcgc ttaacgtcgt
     6001 gaacaaagat gatatagtgc cgaggatcat gcaaaatgga gcgatgaatg ccattaatcg
     6061 ggcgattccg gatcggcgtg ggcggatgca gacagaggaa ttgataagac gtgggaattt
     6121 gataataaac cgaaaacccg acggggtagg gagtccacat tatttaagga actacgtcgg
     6181 tgccattaat gagtgggcta aagatatgag agaaatatgg gacgattatc ggaggacgaa
     6241 atgatgaagc gatttgttaa gctaataatg ctttttgtct ttgcgactgg ttgcggccgg
     6301 gtggaccggg acctgagtaa tgattcccat tatatggggg tcatcggaaa gcaatatcgg
     6361 acgaagatag aacttttggt ttatcggttt aaaggaaata aatttgaaac aattggggaa
     6421 agtggtggtg ggatagaatt gccgcccatc aacgaaacca aggggaaaga atttccctac
     6481 cgttattacg atacatatgt ctttggagtc cttccggcag ggagtgaatt cagggttgcc
     6541 atggtgaggg aagagggggc gtataataac gcattcatct attactatgt cgaaatcaca
     6601 aagagcgatg atcgacaatg ggtggggaaa acagttaacg tttcgtctag aatcattgca
     6661 tccgacgatc cgcttacgtt taaatcaggg ataacagagg aaatgtcgtt tgataataat
     6721 tagaagtggg atcaattctt ggatgcagat gtgaagctaa cataatgaga ataataagtt
     6781 gtggcagttc ggggctacgt tttatgccgg aaatgtgttg acgcgcggaa aaagaagtta
     6841 ggaagagggc atatgtttaa atggttgcgg cgggtggtgt ggggctggct gatgccgttt
     6901 tttgcgcggc atccgttcct gtttttgttg gcgatggggc tgtatgtggt ttcgccgttc
     6961 gatatcgtgc cggaggccct gaccgggccg ttcgggttcg ccgacgatgt gatgctgttt
     7021 tgaatctgta taagtctttt cggcggcaaa ggcaggaaga ggaaaagatc gcccgcccgg
     7081 cggtcatcga cacgaccgcg gaaattaaaa aggacgagtg aaaatgctct ttgtgaaaat
     7141 tggttcagga gcgttttagt caatgttgga ggcggatggg acatcgacaa gaagggggaa
     7201 tttatccgag catttttatg tcttggccgc cggcgttttg ttggcgacca tcgctattct
     7261 tgacctggat tttattctgc gctttgcggg atgggaggcc cgcatgaatg tgattgacac
     7321 agccaagtgg gtggaatggg tccggcctgg ttttttttcg aagaattaca gcaagttcat
     7381 ctttaatttt tcactgattg tattgccatc cttttctatc cttcttgccc actttttcgc
     7441 caaaataaga acaaagaaaa tcacgttatt tcttcttagt tctgccggtt tttttgtttt
     7501 tatgcggtgg ggatatctta aagaggggtt ggggtggaaa cagttgggca ttgtaaacgg
     7561 aattattttg gtggcatatc tactgggctt gatcgttgat aggtggctgc ctcgtcttgg
     7621 cgtgtctcgg gagccgtttt cggttttgga gtttgtttcg gaggggatga cgggggtggg
     7681 ggctggtaaa gtgcggagct tctttaataa atatcaactc tttttggtcg ttccacttct
     7741 ttttgtggta ttgataaatt tcgcttacaa atatagtgct ggcatatgga ttaactatta
     7801 ccatcaaaat ttcctgatag cacccattaa tgacatgctc catggaaaat attttttggt
     7861 tgagacattt tctcagtatg gaatgtttta ccctatcacg ttatttctgg tgttttatgt
     7921 gaagctgttg aagtttacgt acatgaattt ttatttgatc gcgatggtgt tcaccgtcgg
     7981 ttattttctg gttctgtatt attttattcg ctctgttacg cgggggctcg ttttcgccat
     8041 ccttgctttg tttgtggcga tgggagtaaa cacgcttttt aataatgcct atttcccgta
     8101 ttcggagatt tacgattggc ccggggccat gcctctccgg ttttttttgg acgggctggt
     8161 tttttggttg atcgttcgga acaatggttt caaaaataaa attctcagcg ggcttgtccc
     8221 gtgtttggtg gcggctgcgt tgtattacaa ttttgaaaca ggagtgtgtt tggcggccgc
     8281 ctatggcgcg ctttgcctat tgcacgtttt gaacggtcgg gaggggggtg cgaaagcggc
     8341 tgcgtttttg gctaaacaag tcgggtcggt ttccgtcgga gcggtggcga tttatttggg
     8401 tattgtgttt tttacgtggg tgcggatcgg caaaatgcca gaattggatt tattgcttgg
     8461 aattccgctc atgatggcac gcgatgggta tacggcgttg ccgaccccca aaattggctg
     8521 gtattgggcc tatttggcgg tatatttttc tgtgtttgtg ggcgtgtttt attgtgtttt
     8581 gaaaactcgt caaaccagct ggaaatgggt cgtcttgggg gcctatgcgg tttatgggct
     8641 gttccttctc cagtattaca tgaacaaaag tgtgagctct aacttgccgg tggttggcat
     8701 tccctttgcc gtcctggtag ttgttgttac ggaggaggtc cttgggtttt tgtgtcgaca
     8761 taaaaaagcc gactggccat tgtttttggt cgttattgct tttgtgggat ttttggcgag
     8821 tggtgttttg gcagcgcgga ccagttgcta tttggtaaag aaaataaatt acaggattta
     8881 ttcaaaagga cagcttaagc ttttgaaaaa atatgaaaat aacaacggtc tgattgtcag
     8941 ttacagtctt cccccggggc tgatggcgga agatttgctg gccagcgttg cgcgcgtcag
     9001 agagcttacg aagggacaag agcggatatt cttgatttct agacatgata cggtccatct
     9061 cgtaatggca gagaaaacga attatctgcc ttgcccgatg ttggagcaga tttttcttaa
     9121 agagcagttg gcagaattga agagaaattt gataaatgag acgaaaaggc cactgtatct
     9181 ttttacggac agggggtttc ccctgagtcc gattacggcc ccatcgagtc ctaacgcgta
     9241 tgatgttctc gtggaattat ttcagggagt ggaaccctat tatgagttga ttgagcaggt
     9301 gggtgtgata aatatttata gactcaagaa aagcctgggg gcgggagtat gaaaccgagg
     9361 cggttgggga ggcgggtggt gtatacgcac ccgtggctgg acttgtatgt ggaccgggtg
     9421 cggttcccga aagggcggct ggtggaccac cacgtggtgt atttcaagcg ggagagcgtg
     9481 gccacgctcg tggagaacgc cgagggaaag attcttttga tacgatctta ccggtacatc
     9541 accgattcca tcgagtggga actgcccgcg ggcgggtttg agaaggggga aacggtcttt
     9601 gaggcgtccc ggcgggaggt gcgggaggag acgggttacg acacctccgg acatcggctt
     9661 ctttacacct acaacccggt gaacgggttg tcgaacaagg ttttccatgt ggtgcattgc
     9721 cgggcgggga agatcgtcgg aaaattcgat gggaacgagg tcaagggggt gcggtggcac
     9781 acccagaaac aggtgcggga gatgatccgg cggaaccgca ttcacgacgg ttttgcgctg
     9841 gcgagtttgt tgctttattt tttcggcgtg ccggcgaaag aggcgaagaa ttcataaaaa
     9901 aggggacaac gacgcttgat gaatggggcg gctgcggcgg cccacgtttt tgatttggaa
     9961 tgatggtttg acgagagagg cataaggggg cggcgtgacg gattctccgg cggcggtgtc
    10021 gctggcgcat taccttcggg accttttgaa gaacgcggtt cgggacacca ctgcgcggat
    10081 ggtccttttt tccggcggct tggataccgc ggtgttggcg catttggcgt cgcgcctgcg
    10141 cgggttgggg gccatcacgg taacggtggg gggcaacccg ccggacaagg aattcgcggt
    10201 gcgcgccgcc aaacatttcg gcatcaccca cgtgctggtg agccccactc acgacgacct
    10261 gctgggcctg ttgccgcaga ccatcaagct gttgaagacc tacgacccca tgaccctccg
    10321 caacaccctg ccggtgcaca cggcgctgct gatggccaaa gaaatgaacg tgcggacggt
    10381 gctgagcggc gacggggcgg acgagctctt tgcgggctac agttttttct tcgacaagcc
    10441 gttggaggaa ctgccggacg cgttgcggcg gttgtggaag gtgatgagtt tttccggcat
    10501 cgaactggcc cggcggctgg ggatggaggt gcaggcaccg tatctgtctc ctatggtgcg
    10561 ggagttcgcc gagaaggtgc ctcccgggat gctcttggcg gaacgggacg ggaagatttt
    10621 cggcaaggcg atattgcggc aggcgttcga aggggagatc ccggaggaca ttttgtggcg
    10681 ggagaagtgc ccggcggagt tcgggtcggg gacgagcggg ttgaccaagg cgctggaaca
    10741 gaagatttcc gacgaggaat gcgagcggca gcagggcacg ttctggaacg tggacagcgt
    10801 gcgcattcgg aacaaggaac agctttatta ttaccgactc tatcgggcgc agttcgggcc
    10861 gccgaaaggc gtgaaggcgt cggcggggca gggacgttcc tgcccggatt gcggcacgcc
    10921 ggtggcccac aaggaatcgg atttctgtct tttgtgcggc gcctggccgg tgccgtgagc
    10981 ctaaagcgta aaggagagga ttttatgagc ggagcggcgg gcaaaaggat tttttcgggt
    11041 ctggcggccc tgtggttggg cgcggccccg ttgtgggcgg cggattcggt gttcatctcc
    11101 gtcacgcgaa cggacgagag catggaccgg ttgccgtcca acatttcggt ggtgacgaaa
    11161 gaagagatcg cgcagagcgg cgccaagacg ctggcggagg tgttggagac ccaaccggcc
    11221 atggacgtca acaagacggg gtccctgggc acgttctcca ccctgcggat gcgcggggtg
    11281 ccggcctcgt cccaggtgca ggtgctggtg gacgatcgtc cgctgggcgg cacttcgttg
    11341 cagttcatcg acctgtcgca gatcccggtg gagaacatcg aacgcatcga gatcgtgcgg
    11401 ggggcgtcgt cggtgcttta cggcgccaac accatcggcg gggtggtgca cgtcatcacc
    11461 aaacggcaca ccgcgtcgaa gacggagtcc tacgtgggcg tggagggcgg gtcgttccgg
    11521 acgcaggtct atcgggccgg actggggatg aacaagcgcg ggttcgacgc gaacgtggac
    11581 gccagccgct attggaccga cggttttcag aagaacgccg acgccgacaa catcggcgtg
    11641 tccggcaacg gcgggtacac gttcaagaac ggggcgcgga tcgcgctgga cgccggccat
    11701 tccaaccacg aggtgggcga cccgcagggg acgctggttt cgccgtcgga atgggacggg
    11761 tcgcgggagc gcgacgcggc caacacgaag gcccgcaccg aacaggacat caattccggc
    11821 cggttgcagc tcgctttgcc gctgggcgcg gtggggcacc tgcagagtat tttttacggg
    11881 tcggaccagg attaccgcac ctttccggac cgctggaccg agaaggcgga tttcgaccag
    11941 aacaaccgcg tcgtggggaa cgacacccgg ttcttcctgc ccgtggggtt cttggcgggg
    12001 gcgtcgtatc agcgcgaccg gcaggagagc gaaagcgttt acagcgctcg gacggcgcac
    12061 cacgtggtgg attgggcggt gtatgcccag cagcgtctta ccgtcgggcg gttggacctg
    12121 atcccggcgg cgcgttggga ccagaacggc aatttcggca acatcgtgaa cccgcgcttc
    12181 acggcggtgt tccgcgtgac ggacggctgg gacctgtcgg cgaacgcggc gcgttcgttc
    12241 cgcgcgccgt cctttctgga actgttctat gaggggacgt cgttcacggg gaacaaggac
    12301 ctgaggccgg agacgggctg gacctacgat gtgggcgtgc ggtatcggcg gccggaatcg
    12361 gcggtggtgc gcgtgacggg tttctatacg cgcatccgcg agcgcatcgc cgccacctcc
    12421 aaaacctacg agaacctgcc gcaggcggaa ctggccgggg cggagttcga ggcggagggc
    12481 cggttcggtc cgttcaccaa gcgggtgaac tacacctatc agcgggccgt gggcaattcg
    12541 gcggggtcga cccggttcaa gcctctgacg ctcaccccgc gccatgccgc caacctgcag
    12601 ctgatttggg cgatgaaacg gggatggagc ctggcgaacg gcgtgcgttt tgtggaccgg
    12661 cagttccaga acgccacgga tttcgtggga ggcgtgtcgt cgccgcggca catcgtgctg
    12721 ccgtcgtata cgctctggaa cgtgcggttg acgaagagat tgttgggggc ggaactgttt
    12781 ttcgccgtgg agaacatcac caacaagcat tacgccgagg cggtggggag ttattccgat
    12841 ccggtgacct tcgattcgac gagcacgctc tttccgcagc ccggccggac gctgcggggc
    12901 gggatcgtca tccggttctt ggattaggcg cggaattccc cggcggaagt gtccgggggg
    12961 aagggaacgc gggcggcggc ggagcgtcga gcggagaaag ggtcaggaag agctgaggac
    13021 gatatattcg atgtcgaggt tgtcgtctcc gacgttttcg atgtagtagg cggtgtcggg
    13081 cgggatgaga aggtagccgt agttcaattc tttttccgac gagccgatgt aggcgacgcc
    13141 ttttcccttg ctgacgcgga ggatgatctt caggccggcc tcgcgctggg taacggaccg
    13201 atgccctttc gggatggagt aggcgccgat gcggtcgggg gaaatggtgg ggagataccc
    13261 ctgggagtgg tagaagggcg ccacgtccac gttgacctgg cgggggtgtt cctcgggcgc
    13321 gaccacgccg atcaccttgt tgcgggtctt gacgttggga ctttcaacgg cttttaatat
    13381 ctgccagcgg tccttctgca cgacttcgtc gaggctggcc cggcccggcg gggtgttgta
    13441 gataagattt ttttccgtct gagagcggag gtcgtcgtag cgtttgacga tgtcccaata
    13501 cacctggctc tccggcgtgc ccacgcggcg atgcaccagg atgtccttct gcagcccctc
    13561 ataccccgac ttgtactcct cccaataacg tttgaccagt ccggcggctt ccgggttttc
    13621 cggcaccacc cgtccgcggg cgcgccggag ccggacctgt tgggtggcgg tgcggaggtc
    13681 catcacgtcc tgatccagcg cggtgggcgg ggtgctgccg ctgaccttgt aattctcgtg
    13741 gagttcgcgc aggcggtctt cacccatttt gagggtgcgc tggaaatcgt aatgggcgga
    13801 ccggacgcgt tcgccggcga ggacgaagat ctcctgtttt tgggagagga atttttggtg
    13861 cttgccgtcg gcggcgagaa gggccgccgc ggcgaggagg gccgtgggga caaaaaggag
    13921 tttgaagaaa cgcgatctcg tgcggaccat ctttggtgcc attctatcag cctagaagca
    13981 tgaagaaaag aggcttttcc tgacagattt tgatctcccc gtctcggccg cgtcgcggga
    14041 tcgttgcggg ttccggctcg gagggaggat gttttgaatc cttttgggcc ccggcggcat
    14101 ccaagaggta tgggaagagc gttttcaaac aggatgggga gggatgggat ggggccggga
    14161 agggatatga gacgggtgtt gttgggggcg gtgtgtgcgg tggggttggc gggcgggggg
    14221 actccggtgc ggggggagtc tcccgtttca ttggcggagg cggtggaaaa agtattgggg
    14281 cggagtccgg gtctggcgtc ggcgtcggcg caggcgcgtg agcggcggtg ggccggccgg
    14341 gaagcgaagc ggctccgttg gccgtccgtg cggttgcaga gcgcggtgac gcggggcgac
    14401 gggcccgtct acgttttcgg ctcgctcctg cagcagaaat cttttggggc ggagaacttc
    14461 gccctggatt ttttgaacaa ccctcccgac agaaccaacg tgcgcagcgc cgtcgaagtc
    14521 gggcttcccg tgtttgcggg gcacgaactg agcgatgggg agcgtctcgc cgggttgggg
    14581 atggcccagg ccgaaagccg gttggcgtcg gcggttcagc ggacgcggtt cgagacggtc
    14641 gacctgggtt tgaagatatt ggcgttgagg gagaaaatac ggcgattgga cgagcgactg
    14701 gcggacgtgg tcgaagatat tgagagcgcc cggcggttga aggagaaggg gctggtgctg
    14761 ggctccgact attacgccgc cgaagccatt cagtccgggt tgtcggcgtg gcgggtgcgc
    14821 tgggagaagg agctccgggc gatggaaaaa tccctgaacg ttcttgccgg aggggagacg
    14881 tggttggcgg gggctttgaa cgaggagttc gtcgagcttt tacccgacgg cggttgggag
    14941 gagacggcgc tgaagaaccg ggaggagctg cggcaggcgg agttggggcg tgaaatggcc
    15001 gaggtgtccc tccggcgggc gggtcgctcg atcctgccgc gcgtggaggc gttcgcctcg
    15061 gtggaaagca acacccgaaa ttttgagaac aatcccaccg accggatggt gggattgcgc
    15121 gccacgatgg cgttggggga tcccgcttat tcggcccggc gtcggcgggc cgccgcccac
    15181 gccgaggcgg ccgcggccca gaggaacgag acggaggagc gcgtgagttt ggaggcgttg
    15241 cagcagtggg agtccttgca gggagcggtg gagagccttc ctttgctgaa agggctccgg
    15301 gagcgggccc ggaaatccct ggagatgttc cgtcctctgt atcgggaggg acggcagagc
    15361 atcatcgagg ttttgcgggc ggaggaggcg ttggcgatgg cggaaagttc ctacgtggac
    15421 gcgttgtatc agatccattc cggtcaggcg cggttgttgt tggcggcggg ccggttggac
    15481 ggggcggcgg tggcggcgtt ggaccgccgg ctggaggcgc ggccatgagc gaatcccacg
    15541 tgtcgctcgg tctggcgggt cggctggccc aggcgttcat ccgcacgcgc ctgacgccgc
    15601 tgatcgtcgt ttcatctctg ttgatggggg tcttcgccgt ggttcgcctt cctcgggagg
    15661 aagagccgca gatcaacgtc cccatgttcg acgttttcgt gccgtttccc ggcgcgggcg
    15721 tccgggaggt ggaggagcgg atcgtgcgcg tgggagagcg gaaactctgg gagatccccg
    15781 gcgtggaata tatctacagc acggcggagc cgggacacgc cctgttcatc gtgcggttca
    15841 aggtggggac ggacccggaa caggccatga cgcgcattta caccaagacg ttcgccaacg
    15901 tggacattct gccgccgggc gcgggacagc cgctgatcaa gccgcgctcc atcgacgacg
    15961 tgccgatcct ggcggtgtcg ctgtggggcc gggaggtgtc gcccagggcg ctccgccggg
    16021 cggcggcggt cctgcgccag gagatcagcg cggtgccgaa cgtttcggag atcgagatca
    16081 ccggcggccg ccggcgccag ttcttggtgc acttcgatcc ggaaaaactc gcgtcccggc
    16141 ggctgacgcc gctggaattg gcgggaaccc tccgggcgtc caacctgcgg cttccggcgg
    16201 ggcactacaa ccagaacggg aatgccgtgg ccgtggaaac cgatgcgttc atccggacgt
    16261 tggacgacct gaagcgcgtg gtggtggggg tttccggcgg ccgtccggtc cttttggagg
    16321 aggtggcgcg ggtctccgac gggccggacg aggacgaggc cgaggtgttc ctggcgcccg
    16381 tgcgcgtggg cggggtgggt ggcgacgaga cttttccggc ggtgacgctg gcgatttcca
    16441 agcggcgcgg cgccaacgcc acccacgtgg cggaggaggt ccttcggcgg ctggaacgcg
    16501 cccggtcgac ggtgctgccg gaaaaaatag agacggtggt gacgcggaac tacggcgaca
    16561 ccgccaagga aaaatccgac gagctcctct tccacatgct gttggccact ctctccgtga
    16621 cgctgctgat cgccctggcc ctgggtccgc gggaggcggt ggtggtgttg gtggccatcc
    16681 ccgtgacgct ggcgctcacg ctgcttttct attatctggc cggatacacg ttgaaccgca
    16741 tcacgctttt cgccttgatc ttctccatcg gcattttggt ggacgacgcc atcgtggtgg
    16801 tggagaacat ccaccgccat tactgcatga acgacggccg tccgttgtgg aagctggcgg
    16861 tggaggcggt ggcggaggtg ggcaacccca cgctgttggc cacctggacg gtgatcgccg
    16921 ccattttgcc catggctttt gtgggcgggt tgatgggacc ctacatgcgg ccgatcccgg
    16981 tgggggcgtc gctggcgatg ctcttttccc tggccatcgc cttcatcgtg tcgccgtggg
    17041 cgttcgccgg acttctcaag ctgtggaagc cccgggtgcc gtcttccggc gagaaggagg
    17101 ggcggctgga caagctctac cgacggtcca tgcgggccgt gttggagagt tcgaccaaga
    17161 ggaacctgta tttcggtctc atggcgttgc tgctggcggg atcggtgttc cttctttttt
    17221 ccaaaaaagt gacggtgaag atgctgccgt tcgacaacaa gaacgagttc gaggtggtgc
    17281 tgaccatgcc ggaagggacg tcgctgagca agacgcgggc ggcggcgggc gagatggcgg
    17341 aggtcttgcg ggacatcccg gaggcgaagc aggtggtggg ttacgtgggc acctccgccc
    17401 cgtacaattt caacgggttg gtgcggcatt attttctgcg ccgccggccg caccaggcgg
    17461 acctggtggt gtcgctgacg ggccgaaagg aacggaaacg gcagagccac gccatcgccg
    17521 cctcggtgcg ggggcctctg cgcgagatcg cccggaaata cggcgcgcgc gtgcaggtgg
    17581 cggaggtgcc gcccggcccg ccggtgttgt ccacgctggt gctggagatc tacggcccgg
    17641 acgcttccct ccggaaaaga ttggccgcct tggtgcggga actcctcgaa aacacggaag
    17701 gcgtggtgga cgtggacacc tacgtgccgg acacgcagcc cttggtccgg ctgatggtgg
    17761 accgggagaa gaccacgctc aacggtattc cggcggcgct ggtggcgcag accgtggggg
    17821 cggccctcca gggcgagacg gtggatttgg cccacgtgga aggggagaag gaaccggtgg
    17881 agatccggct tcgcctgccg gaggagcggc ggcggtccct ggcggcggcg ggggccatcg
    17941 cgctgttgac tcggaacggc acgtcggtgt ccatcaagaa tttaacgaag ccgcggatcg
    18001 tggaggtgga tgcgcccgtg tatcacaaga acctccaggc ggtggattac gtgatcgccg
    18061 aggtggcggg ggaacaggaa agtcccgtct acgccatcct caaactgtgg ccggagatcg
    18121 acaagctcgc cgcgcaacac ggcgtgggcc tgaaacaata tttcgcccgg tcgccggtca
    18181 attcctccga gaacgcgttg aagtgggacg gggaatggca catcacctac gaggtcttcc
    18241 gggacctggg gatcgctttc gcgctggtgc tgttgctcat ctacgtgttg gtggtggcgt
    18301 ggttcgattc cttcaccgtc ccgctggtga tcatggtccc catcccgctg acgctggtgg
    18361 gaatcttgcc ggcgcattgg gcgatggggg ccttcttcac ggcgacctcc atgatcggtt
    18421 tcatcgccgg ggccgggatc gtggtgcgga actccatcat cctggtggat ttcatccaac
    18481 tgcgcctgcg ggaggggatg cccctcaagg aggcggtgat cgacgcgggg gcggtgcggt
    18541 tccggcccat gttgctgacc gcggcggcgg tggtggcggg ggccggcgtg atattgttcg
    18601 atccgatttt ccaggggctg gcggtggcgt tgatggccgg ggagatcgcg tccacgcttc
    18661 tttcgcggac cgcggtgccg gtgctttatt atcttctgag gaggagggcg catgagcgtt
    18721 gaacaccgga tccggctgat cgccggaacg ttcatcctgt tgagtttggg aggagcgttt
    18781 tggttggacg cgaattttat ttggttcgcc gcgttcgtgg cggtaaacct aattcagtcc
    18841 gccttcagta aatggtgctt gatggaggac ctgttgaggg cctgggaccg ccggcggacg
    18901 gggaaaaccg atttctaggc gtcggtcctt ttgaaccccg atttgatcca gccccagttc
    18961 aaggcgacgt ggccggccac cgcggcgatc aggacgagac cggcgccttc gtgaagcacc
    19021 tcgaacgtct ctgcggaaag ggcgcggtga aaaaggccgg tgagggcctg gttggcgaaa
    19081 aggagcccga ggaccgggtt gaggatgtgc agggctttgt gacggttcat gtgggacctc
    19141 ccatgttctg aaacagcgcg tgggatgtat tctatcagaa ttgggcgccg cgaaagtttt
    19201 ccgtcggcgg cgtcttttgg ggaatgttta ggtataatgg ttttgcggga acctgaggcc
    19261 ggaggcgggc ttcaggtttt ttaatgggcc gccgccggag aagccgcctc gtcgatggaa
    19321 gccgcgccgc aggacggaac gccacggagc tcggaacaag accatggaat tatttctgaa
    19381 aaacctgatc ctccccaccg ctctcttcga taatcttcgg ctgatcatcg tcatcctcga
    19441 cgaggacggc ttcatccgat acgtcaaccc ctttttctgc gaaagcaccg gctatgcccg
    19501 gaaagaggtc gacggccaaa gctggttcga gaagtttctc cccgacgaaa acattgagac
    19561 ggtgatgggc gtgttccgcg acgtgctggc caaggaggaa tacgtccatt tcgacaacac
    19621 catcatccga aaagatggga cgaagatgtt cgtctcctgg acgaattcgg tgcttcggga
    19681 aaaggacggg aagtcccggg ggacgctgag tttggggatg gacatctccg gccacaagga
    19741 gatggaggac gaattaagga ggagcgagga actctatcgg gcgttggccg aatccacgct
    19801 ggacatggtg tatctggtgg atttcgacgg gatcatccgg tatgtgaatc ctctggcgtc
    19861 ggcccagttc ggggtgccgg cggagaagat catcggcaaa cggttggcgg aagtcttccc
    19921 gccgcatttg gcccaaaggc atctgggcgt gattcgaaag atttttgaga ccggagaggt
    19981 ctattccggg gagcttctgg aaaagttccc cggcggcgag gtgtggatcg acgcccggct
    20041 ctcgccggtg cgcaaccagg agggaaaggt ggtctcggtg ttgggggcgt ctctgaacat
    20101 cacggagcgg aagagggtcg aatcttctct caaagagagg gacgagctct taacgggggc
    20161 gcaacagatc gccaacctcg ggtcctacac gctggacatc gccgacagcc ggtggacgag
    20221 ctcggagata ctcgacaata ttctagggat cggcgacgat ttcccgcggg accgggaggg
    20281 ctggaatttg ctggtccatc cggaagaccg ggaatggctg gcggagtatc tgaacagcca
    20341 cgttttgaag aagaagaacg ccttcaacgc gcagtatcgc atcgtccgat attccgaccg
    20401 gcaggtccgc tgggtgtacg ggatcgggaa attggtgctc gacgagaagg gagaggtgac
    20461 gcggttgctg ggaaccatcc aggacgtgac cgaacagaag aaggtggaag ccagcctgcg
    20521 gaactccgaa cagcgttacc ggaccacgct ggattccatg ggggacatga tccacgtggt
    20581 ggaccgggac ctgagaatcc ttctggccaa ccgggcgttc gccgagttca accgggccca
    20641 cgggctgaac tccgacatgc tcggccgcac ggttttcgag gtgttcccgt ttttgccggc
    20701 cgtggtggcc gatgagtata aaaaagtgtt tgagtccggc cggcccctgt ccaccgagga
    20761 gacccatcgg atcgacgggc gcgaatacaa caccgagacg ctgaagacgc cgatctttga
    20821 aggggagaag gtcacgcagg tgctcacggt gatacgggac gtgacggaac gcaagcaggc
    20881 gcagaaaaat caggagctga tgcagaagga gatcctgaac gccctgacga aacaacagat
    20941 catgatcggc cagacgctgc acgacagcct gggacagttg ctgacgggcg tggcctatat
    21001 ggtcaagggg ctggaggagg accagaaggc ggggcggccc atctacgcgg aggaaacggc
    21061 gcgcatcgtg gaatacatca atcaggggat cggtcagacg aagagtcttg cccgaatgct
    21121 gtatccggcg gagttggaga ggggggggtt gccggccgcg ctggaaaacc ttgccaatca
    21181 gacgcgcggc atctacaatg tgggctgttc ggtgcgcgtg gagggggact tccaggtccg
    21241 gagcgatgtg ctttgcacgc acctctatcg catcgcgcag gaggcggtcc acaacgcgtt
    21301 gtctcacggg caggccacgg atatccacat cgccctggcg gtccgcgggg cggaaggcga
    21361 gttgtcggtg gcggacaacg gcaaggggtt cgatgagaag gaggcggggg aagggaaggg
    21421 cttgggattg aagatccttc aataccgggc gcaggagatc ggggggagcg ttttcgtgcg
    21481 gaggaactcc ccgcgtggga tgatcgtcgg atgtcaattc aaagtggagt aagagacatg
    21541 gcgcatgaac atgtgaaatc cgtttccaaa aagaagatat tcgttttgga cgaccacccc
    21601 atcgtgcggg aggggatggg gcagctgatc aaccggcagc cggacctggt tttttgcggc
    21661 ggcgcggcct cggtggccga ggccgtccgt tcaacggctc agttgaaacc ggatatggtg
    21721 atcgtggatc tttcgctgcc caaagagagc ggcatggatt tcatcaagga gctgcgttcc
    21781 gcccatccgc gggtgggcat tttggtggtc tccatgtacg acgaattcat ctacgccgaa
    21841 cgcgttttga aggcgggggc gatgggatat ttgatgaaac aggaggccac cgaccggctg
    21901 attggggcca ttcgcacggt gctccagggg aagatttatc tcagcgaacg gatgcggtcc
    21961 gaaatcgtga cgcgcaccgt gggcggcggc ggggaatcgg tttcgtccga ccccatcgcg
    22021 aagttgagcg atcgggaatt ggagatattc cggtacatcg ggcagaaaat gtccacgcgg
    22081 cagatcgccg aaaaactttt cctcagcgtc aagaccgtgg aggcccatcg ggagaacatc
    22141 aaacacaaac tggggcttta ctccgcggcg gacctgtttg aaaaagccct cctttggacg
    22201 aagatggaaa aatgacgccg cggcgggggc cgccctcgcg gagatgattt aaatcgtatc
    22261 gttttttcac atcgttcgtg tagttgatcc gtcctgccct ttccccaaaa catcgaaaag
    22321 ataggtaatt cccctatgta gactaggggt tcgtcctatt taaaatagtg cataacccga
    22381 tggcgagcgg acgcctcttc cggcataatg tgactgttgg aatgtggtat gagtgacgtg
    22441 acgaaagatc ccgctgtgta ttattggcgc cgcgtgcgcc ggtcgtcgtt gacaggaaaa
    22501 gccgaagagc ggacccattt ggaaatccgc ccggccgcct ccgagacggc gccggcttcg
    22561 ccgataaaaa ttttgccgct gccggcaaaa atgcgaggac gggggcgtcg aacgggctct
    22621 tgaaaatttg agggaaacaa ggaagttcgc gggtggggca ttgtcctaaa aagggacaat
    22681 gccccaatgt gcgtacgggg gggccgcggc cacccgtttt ttaaaaccga aaagacgccg
    22741 atgtcccctc cccaaaaaaa tccctgatcg aagaacatcc cgtgattcgc tccccaaggc
    22801 ctcctcgtgc tgggttcgcc aagggggaga gagaaatgta taataacgga cgatattttg
    22861 aggagccggg cggggctgct ttggggaatt tgttacaagt agtagtctat aacacggaaa
    22921 gagttttacc atcgggcccg cccctcaggg caacgcgagc cggccgttgt gtcgccacga
    22981 ccggtttttc ccggaggggc agggccaagt tgcatgctaa ttgtaaagcc gggtccaccc
    23041 tcgctcatgg acagggtcgt tgtttaaaca gaaaacatcg accagtcgcg aggacagggc
    23101 ccagtcaaca atggtagcta cgggcccgta gctcagttgg atagagcatc cgccttctaa
    23161 gcggagggtc ggcggttcaa gtccgcccgg gcctgcgttt tcccgaacga ggagcgaaaa
    23221 agcggtcctc gattcgaaac tgcggcagtc gaggaggtca tctatggaag cttgggccaa
    23281 agtgctgctc ggcctgggtt ttttgtttct gggtctatgt tatctctacc ggccgagttg
    23341 gattctccga ttcaacacct ggggccgcgc ggc
//
LOCUS       DAOTFD010000021        20096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1767_length_20096_cov_4.025548, whole genome shotgun sequence.
ACCESSION   DAOTFD010000021 DAOTFD010000000
VERSION     DAOTFD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 20096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20096
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1767_length_20096_cov_4.025548"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2428)
                     /locus_tag="P5079_02545"
     CDS             complement(<1..2428)
                     /locus_tag="P5079_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY28898.1"
                     /translation="MTKFLTVTSGAALVGAGLIGYFAKSGVQNAILAEVGERSAALVR
                     PLAKGPEMTAAFQSGEERKFLPLLLKMQSVTGAAGVGAFNQLGKVLAHTNVSKTGRLW
                     DDPLLREGIRSDKIVRRVLMENDPPVLVLTVPVWRHVLSGSEEELLGARERLGTIRLS
                     LDISRALRVAWRESRRASLIIISISLVALLALMLFARKFLAPIARLSSAVDQVAHGGL
                     GEMVSAESHDELGDLARSFNRMSQALKVTSVSRNYLDGVLENVADMLIVTDLLGNMEK
                     INSAAVSTLGYEAAELLHQPIGVLFAEFDKNATLEHLSAGETVKNLEMQMVRKDGTVL
                     PVLYSMSALVGRDNKPVAIVSLAKDMTEQKRAESSLRASEEKFRRLVENLRQNYFFYR
                     HDTGGVMTYLSPSITQVLGYDPEMFLTHYSEYLTDNPINREVERHTNLSIQGIQQPAY
                     LVEIYHKDRSVHLLEVTETPVFGPDGRVAAVEGIANDVTARHLAENRLKALNDCFLNF
                     GADHVKNIQSLTVLCGNLLVSDGAFYQRLDKGSLCLVSGWKTPPDMPSEGKAEGRICA
                     SVIRENADEPVILTDLPHTPYAESDPAVRKYELKTYLGRAVKCRGESIGSLCVVYRHD
                     VRPTEEDRKFIGILASAIGVEEDRRAAEELVRQARDFYLTLFEEFPTFIWRCGVDGKC
                     NYFNKTFLTFTGRTAEREMGEGWAEGFHPDDFAKCQNAFSEAFKKREPFEMEYRLRRH
                     DGVYRWVLDAGRPFYDMDKKFAGYIGSCLDITDRKNMEAGLAQKEKLAAVGQLAAGVA
                     HEINNPLGVIL"
     gene            complement(2434..3321)
                     /locus_tag="P5079_02550"
     CDS             complement(2434..3321)
                     /locus_tag="P5079_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRY28899.1"
                     /translation="MKRKNGIGSALSLCLAATLAVSAAADEASVAAVVASNTGPYAAA
                     LEGFREGMGPGGVAVFDLSHGAPSLPSSAKVVVAFGGKAAMENYPEGTTLIYCLAPGT
                     WIDPAQRKGQTVMVSMETSFQTIILELKEIQPNLKRLAVLWGSAHLSGLAKSRRVDES
                     GVRVLPVEVTSSDELPDVLRGLEGRVDAVWLMADPLLLNEQNVRMIKEFCWANKLPVY
                     SSVKRFLDYGSVAAVYVDFGDIGRAAANAARRALAGESLSGDIASPRFDIAFNMTAAG
                     ECGVNISEDLKRRATAVAP"
     gene            complement(3318..5288)
                     /locus_tag="P5079_02555"
     CDS             complement(3318..5288)
                     /locus_tag="P5079_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY28900.1"
                     /translation="MKRRIGTFFIAVFFAAGNAYPSDEVFDFFKSEAEVVTAAKHPQK
                     ISRVPAAAYVLSAEEISLYGYRTLGEALQSIPGFYVINDRNYTYLWVRGFGRPGDYNS
                     RVLVMINGHRINENSYGGAYLGHDFNLDIRAVDRIEVVKGPGSALYGDSAFFAVVNVF
                     TKRPSASPEVFVDASGGSYGTNREFFSWTQPVGPRGGIFAAGSYRWMEGQDLSYPEFG
                     AINGGIAENADREESYTGFASFTRDGLSIQGNSNGRRKRIPTASFGTRFNDPGSWTVD
                     ARRFLETKLERPLGGAVNVYGRVYADHYRYVADYVYDGPFPETRLVNRDLAESEWYGE
                     ETRLSVNVAGEENVFLVGQEFEKNVEGLLRNHDVEPYALYTDVHAALRRWAVFFQQEL
                     KPARFLSLTAGGRYDHYQSFGKSVNPRAAVIVQPNRRSSFKLLYGRAFRAPSVYEMFL
                     DGIGYRRNPQLRPERITTYEARWERRLAGGGPQLSAGYYRSDVHDLISQTVDPSDGSL
                     VFQNMEEVLSKGGEFEARWDVSSRLSARLGYIGQSTREIGGGRLSNSPRHSGVAGLSR
                     RFSTWRASLGLETFYVGRRTSYQNTRVPGDVVISLNAALSPWEDGPRFTLGVYNLTNT
                     SSLASGDGGLVQATIPQDGRNFQVGVEYKFRP"
     gene            complement(5303..5875)
                     /locus_tag="P5079_02560"
     CDS             complement(5303..5875)
                     /locus_tag="P5079_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28901.1"
                     /translation="MEKKAYGTHDIARLCHVTPPTVGRWIEEGKIPSFVTAGGHRRVW
                     DSDLAVFLKSLKMPVPEELGVKPPTILIVDDEESVRKFIKRVLRNVYGSEVAAEEAVD
                     GFEAGKKMTELRPALVVLDVLLPGLDGERVCRMIRADDDLKGVKVLAISGINVDQTKK
                     RMLAAGADDFLGKPFGVEDLEKKLRAFLPA"
     gene            complement(6232..9675)
                     /locus_tag="P5079_02565"
     CDS             complement(6232..9675)
                     /locus_tag="P5079_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY28902.1"
                     /translation="MKDAEARNIARRHLHENVVVEAGAGTGKTTLLTDRLLFLLLAGG
                     PEKKGIALPRIVALTFTEKAAGEIKVRLSARLNDLVAVLEGRDLPTEERTRKAAGLLE
                     ELKTHFGRPPASVRELAEKALHEMDRAEIGTIHHFASRVLRLYPEEAGVDPNFKVDKG
                     EFFDEFFQSEWAAWLDRELGTEAPRAGLWLEVLPLTSLEDIALVARALCRDASEEIPL
                     EISSRVKERIESLSRDVGALAVGQRDPGANSKMPESLAKISQELRILAASYGVKAPLP
                     AERTYRDFSKEKSRKWPKSWTPAAEEALYQEGVAVVMQTSAVAESLVRKVVDLVRPFA
                     GAFRGRYTRKGHVTFDGLLLTARALVRDRPEVREALKARFETILIDEFQDTDPVQGEF
                     LLYLAETAGDSASSWENIRYSPGKIFVVGDPKQSIYRFRGADIRAYKRFTESILAQGG
                     RKCDLQTNFRSLESLIRPVNAVCSAVMREDPKNPGLQHAYLPIYPCPENEEKTLAAGR
                     RGEGKTPEAALELVLTRPGDDGVLSSADSQQMEARWMTRWIQKNCLAAADGPAPRYRL
                     RDVAVLLRTTTPLNVYLDVFKESHIPYVVESDRYFYGTQEVIDFVNLLRVLDDPHDRV
                     SFLGLLRSPLVALDDQDTYRLRFARKPAVPRPTDEGDPEGLDAFTYLQDPPEEAALSA
                     GARARLAAFFDFLRKTRARVGREPLGVFISRLLKESFLLELASVAYHGEQTVSNLQKF
                     GRLAAEAGESRGVTLKEFIEFVVRSMEETAEEGESPLADEHLDAVRLLTIHKAKGLEY
                     PVVFLPNISADVTGGGEGRPPFRMDWGEGVAGFRLVSAKSADIAMAILERDEKVREAA
                     EWVRLFYVALTRAKEKLILLGRAEGGQRSSFGRMLKDAAAWPADDAAEAVFGDGGRFP
                     VWNIRKEREEKIYKKKIRGAPRIVEAKDLAVVWRRRYDEFSRWQDRVLFDSPTRSPHE
                     REKPVFEAGEAFLSENVAALVGEVCHRVLEAWDYRRGGDLPALTRQACRRLRRHHPVS
                     DWDRVEKESQTLLASFLGSPAGKRLAAGEVVGKELPFIFSSDGSIMRGSIDLIYQENG
                     RFWVADYKTDRISPQDIPSRLERYRPQATVYREAVRRATGRECGFQLIFLRQGEIVEV
                     P"
     gene            complement(9672..12974)
                     /locus_tag="P5079_02570"
     CDS             complement(9672..12974)
                     /locus_tag="P5079_02570"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRY28903.1"
                     /translation="MPPAIICAPFQPCLEKTLMDTLSLHPPGPGRLVAVVSPSRRMSE
                     RLQRLIAREQNLPLLNIRFHTFFTLAMEVLDGSAEPDRVVVGDELFHDKLVDSLLRAA
                     GRETRRLSPGLSAAYRASIRDLAEAGVEPALFREHFSDVLKTGDLGRRLEFLLTLQEK
                     YLERLEEMSVLPVAGLERVATRLLEEGKSSALEKYGEFLYYGFYNLNGTQADFFSSVV
                     RMYPATVFFPYRKDHPAFSFAERFYQLKLPVGGAEPIHLRGRSPDGSAKKKSRPRLPG
                     GAPCEGPPALGRALDGLFDPVQPAVTVPEEKLKIINVSGLRDEVWRVAKEILALRERP
                     KNPVEFDDIGIVARSMDPYRGVLAEVLAENGIPFSLRESEPLRMHPIAKLTLLFLTLR
                     RRDYPAGAVLDVLESPYFRLDRFQGRVRGGRLLDHWKQLADRLGVHSGWLQWRGKLAP
                     WAKKDFELFPQLAAEGRPGYRIAREDTAALWNFLEDLHRRLAPTELQGWTRLADYARA
                     LLEENFKVEAGDPDAPAWAATLEVFEDLKAFDMLDPNAGWDVFLETLEEKLMRAGLEP
                     PEGRRGVRVFNAMDARGDSFRVLFLIGLREGFFPRQVREDPLLRDSLRALLQQPGGYW
                     ISPRLGGYDEEKLLFYLMVSSAREKLYCLYARSDDSGKAQVPSIYLRELCRAAGRDLE
                     SDPGERVPRQPWAKLDALPPSFLSPKEAALLLARDGEDPGPLLEPLGTDSKLLKDCLD
                     RVTDMNRFGEPHALDGIVGHTEGVVEGLLTRGLSPTAMDVFGTCPFQFYASRVLGLRE
                     IDEATDRSDLARTVRGRIYHLLMQRFHEKIAAQGYWKKPSSAAGDWVPLLKEVMESVF
                     REFSWEGTGTYPLFWESVRRQMAVNVRRVLERDMAELTESGYVPSVMEKELRVPAPLE
                     LTGPMEKLEFRGQPDRVDVQVSEKKFRIIDYKTRWPGWKKSLASYVLSSQAHQPPVYL
                     EMVSHPKSGLVRGLSPSGVCYYIMEDSPSLTGAEPVQEFPAEVWKAHRSALLKNLAGF
                     VQQMERGEFFIVPKEGQGGHCTHCPFGAACRKSHPPTRRRAEESVLREEYEKLKSVEP
                     KKKKTPAEAEES"
     gene            complement(13032..13116)
                     /locus_tag="P5079_02575"
     tRNA            complement(13032..13116)
                     /locus_tag="P5079_02575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13081..13083),aa:Leu,seq:taa)
     gene            complement(13544..13885)
                     /locus_tag="P5079_02580"
     CDS             complement(13544..13885)
                     /locus_tag="P5079_02580"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRY28904.1"
                     /translation="MRWQLRSKIHKAIVTEADVNYVGSITIDEDLVERTGIWPGEKVL
                     VASNTTGARLETYVIVGKRNSGTICMNGAAAHLIKVGEEIIIMGFEMTDQPIVPKNIL
                     VDKKNKFVKNL"
     gene            complement(14004..14825)
                     /locus_tag="P5079_02585"
     CDS             complement(14004..14825)
                     /locus_tag="P5079_02585"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRY28905.1"
                     /translation="MTIHPPRFRRVKSVAGLLAVLALQAFVPLRADAPAFAWRRETNR
                     AFGAGEKISYRIRYGMMTGGSVLMEVGGPQRLAGRLAYRIVSRASTNKMLDRVFKVRD
                     LNESWMDAESLCSLGFVQSVREGGYYKETATAFDHPSGIFSYVFKDHPFDSRLGQPFP
                     PFTQDILSSLYYARTREWTVGSEIIFDVNSGGRNWPLKLTVQGVETVQTPAGKFECWR
                     VEPTLAGEGLFKSKGRLVVWFTRDERKVPVLLQSEVAVGAFQADMTEYVPGNPQG"
     gene            complement(14822..15226)
                     /locus_tag="P5079_02590"
     CDS             complement(14822..15226)
                     /locus_tag="P5079_02590"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRY28906.1"
                     /translation="MPAIVWDDSLRTDIELIDGQHRELFERANRLFEAVGRGKNDKAV
                     GEALQFLMGYVKRHFHAEEKFMRAHAYPKLSAHSWQHTDITQSLNDLVAQFKRGGDMW
                     IAMKVSRLFSGWIVNHIRQSDMDYVRFIHRKK"
     gene            complement(15251..15745)
                     /locus_tag="P5079_02595"
     CDS             complement(15251..15745)
                     /locus_tag="P5079_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRY28907.1"
                     /translation="MKEISPADIRVNPFKLFGSDWTLITAGTKKSCNTMTASWGGFGV
                     LWDKNVCFCFVRPVRHTYQFLEKNDRFSLSFFDEKYRSALNYCGSNSGRDTDKIAGSG
                     LTPVEEFGTVYFKEARLVVICEKIYFEDFDPKNFRDPKIAAFYPLKDYHRMYLGGIVT
                     CLAG"
     gene            complement(15749..>20096)
                     /locus_tag="P5079_02600"
     CDS             complement(15749..>20096)
                     /locus_tag="P5079_02600"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28908.1"
                     /translation="VTGAGLAYGFTQGLFQREMSERLWFGLAIGLLASVGVSVVRDAV
                     NAVRRKGRLQSWRLYFIDALMGGFIGSAMAFYLDSSQVPVIVEKFKLYNSAGLEPKDY
                     TIYPWLSKWGRVDLGHFSGGVKLLYNEALAGVIGWAIAAWLFAVNRTFMEAYFQKDNA
                     PIKHFFSKAGFVELTMNMIHVLRWGLWMSPIINTFLRMMAKATWYNQDGALRTLVAVY
                     HNITLSPSDFQAWSLQVFVALLSYDLVRVLIWLDHMGLRVATLVNLSFIGMNKLDERV
                     ARFIGRSAAQQYIPEGVKRFTTWAPLLIPFYIPRGEAWDYAWNKSQEIQNAAQGGGLV
                     SAARALTLSQWAVLALAAVAAGTVVSFVLRSFRRRAALRREKVYELVNREYRLAIGEN
                     GEGHSEYLSKGYDLTRRSYDPLHPCGRALFVVDTVSKPGNPSRSWPVMGNFPKEKFEP
                     SRVEQAEDALRVVNTHNGIRTTVTMSLPDPDVPAEIVDVTLENLTDAPRQLKVVPYLE
                     WVLDRPDADKGHTQYNRLFPEMEYAADVNAVLAWSKHSKSMGLLASERSPEGFLSSRM
                     DFIGRARSLWTPRVLETLGFLEARAAAAYPTFDPIASLSLPVALPPKSAESLRLMIGC
                     AKNRENALAFIKKYLRPTPSSERKAVAREASRKGAKAPLIGHGEILPGTPQPYSEFTE
                     NGNKLFVRTPYTPRPYDHAMSNAIGHTVMVTNRGLHTSMNGNSQQNPITPDWPDTVTR
                     EVSAEAIYLFDRDENEWYCPTLHPLNDHSAKHECEFGVDGTALFRMTRGTVSTELTVF
                     VPPADSTGVYLLTIKNNSDKTRRFRLAPYFQVVLTGQAERRHGPLQVAHDRALNAVFF
                     ENPRNSFRTGPAFAALSVPSDRVETERGRFFGPGRGPDHPFMVEKGVPADLRLDEDRP
                     IAGFLATVDIPAKSERTVVVLLGQVDDRKQAAALIQKYKTVESARARLLETRKWWLSL
                     METVKIRSNQPEFDQLLNWLKYQGIAERLWARRGFYQTSGAFGFRDQLQDSVNLVWVD
                     PALARKQIILHASQQFIEGDVVHWFHTLHDGRTAFSNRSHASDNLLWLPWAVGEYVRT
                     TGDETLLEEMTSYLRSENPFQALPKMKHGWGSVYQRSTREDTVYRHCMKAIDLVLDKR
                     MGAHGLPLIGTGDWNDGLDEIGSQGRGESVWLGFFLDYILKAMLPLIEKKDGPKRKEH
                     YAVRLKSLEESLEKTWREDRYLRAIHDDGTEIGVKDSGVWEIDALTAAWAVMAGINPE
                     RSLTVFQTALRVLEKENVILLGWPALREDTKPFLGRSSHYPEGVRENGMYSHGVQWLV
                     KAARILAERFAQQGEAAKAKEFRAAAYRLWRKISPLDHVTPDQIELYGGQPNKQAADM
                     LTTFDQGRMIWHGYTGAAGWMFRQAFEGVAGASLLKNEVILPLDMEEPRGDLKVNQVV
                     RDLGSSPLRGGR"
BASE COUNT         3841 a         6666 c         5826 g         3763 t
ORIGIN      
        1 cgaggatgac tccgagagga ttgtttattt cgtgcgccac cccggcggcc aactgcccca
       61 ccgccgccag cttctctttc tgggccagac cggcctccat gttctttcgg tccgtgatgt
      121 caagacagga cccgatatag ccggcaaact tcttatccat gtcatagaac ggccggccgg
      181 cgtccaaaac ccagcgataa accccatcgt gccggcggag acggtattcc atttcaaacg
      241 gttcgcgctt cttaaaggcc tccgaaaaag cgttctggca cttcgcgaag tcgtccggat
      301 ggaacccctc cgcccacccc tcgcccattt cccgctccgc cgttcggccg gtgaacgtga
      361 ggaacgtctt gttgaaatag ttgcacttcc cgtccacccc gcacctccaa atgaacgtgg
      421 ggaattcctc gaacagcgta agataaaaat cgcgcgcctg gcggaccagt tcctccgcgg
      481 cccggcggtc ctcctccacg ccgatggccg aggccagtat tccgatgaat ttccggtcct
      541 cttccgtggg gcgaacatca tgccgataaa cgacgcacaa actgccgatg gactcgccgc
      601 ggcatttcac ggcacggccg agataggtct tcagttcgta tttcctcacc gccgggtccg
      661 actcggcgta gggcgtatgg ggcagatcgg tcaggatcac gggctcgtcg gcgttctctc
      721 gaatgaccga ggcgcagatg cgcccttcgg ctttcccttc ggaaggcatg tccggcggcg
      781 ttttccaacc ggaaacaagg cacagactcc ccttgtccag ccgctgataa aaagcgccgt
      841 cggacaccaa caggttgccg cagagaaccg ttaaactctg tatgttcttg acgtggtcgg
      901 cgccgaagtt caaaaaacag tcgttcaggg ccttcaaccg gttttcggcc aagtgccggg
      961 cggttacatc gttggcgatc ccctccaccg ccgccactct tccgtccggt ccgaagacgg
     1021 gcgtctccgt gacctccaaa agatgaacgg accggtcttt gtgatagatc tccaccagat
     1081 acgccggctg ctgtatcccc tgaatgctca ggttcgtgtg gcgctccacc tcccggttga
     1141 tcgggttgtc cgtcaggtat tcggagtagt gggtcaagaa catctccgga tcgtatccca
     1201 acacctgtgt gatcgacggg ctcaagtacg tcataacgcc gccggtgtcg tgccgataga
     1261 agaaatagtt ctgtcgaagg ttttccacca accgccggaa tttctcttcg ctcgccctca
     1321 gagacgattc cgcccttttc tgttccgtca tgtcttttgc cagtgaaacg atggcgacgg
     1381 gcttgttgtc cctgcccacg agagcggaca tggaatacag cacgggcagc acggtgccgt
     1441 ctttgcggac catctgcatc tccaggtttt tcaccgtctc cccggccgac agatgttcca
     1501 aggtggcgtt tttgtcgaac tccgcaaaaa ggacgccgat gggctgatga agcagttccg
     1561 cggcctcata tcccagcgtg gacaccgcgg ccgagttgat tttttccata ttgccgagga
     1621 ggtccgtgac gatcaacatg tccgccacgt tctccagaac cccgtccaga taattccggg
     1681 aaacgctggt gaccttcaac gcctggctca tccggttgaa actgcgggcc agatccccga
     1741 gttcatcgtg ggactcggcc gaaaccatct cccccaaccc gccgtgggcc acctgatcca
     1801 ccgccgacga gagccgggcg atgggagcca ggaatttgcg cgcgaacaac atcagcgcca
     1861 aaagggcgac gaggctaatg gagatgatga tcagcgaagc tctccgcgat tcccgccagg
     1921 ccacccgaag agcccgcgag atgtccaaac tcagacgaat cgtcccgagg cgctcacggg
     1981 cccccagaag ctcctcctcg gaccccgaca aaacatgccg ccagacgggg acggtgagca
     2041 ccaggacggg tggatcgttt tccatcaaca cacgccgcac gatcttgtcg gaacggattc
     2101 cttcacggag gagggggtca tcccacagac gtcccgtttt cgacacgttg gtatgcgcca
     2161 ggaccttgcc aagctgattg aacgcgccga cgcccgcggc cccggtaacg ctctgcatct
     2221 tcagcaaaag cggaaggaac ttacgttctt cgcccgactg aaacgccgcc gtcatctcgg
     2281 ggcctttcgc caggggccga accagcgcgg cgctgcgttc ccccacctcc gccaaaatgg
     2341 cgttctgaac gccacttttc gcgaaatagc cgataagccc cgcccccacc agcgccgcgc
     2401 cgctcgtcac ggtcaaaaat ttcgtcatta atctcatggc gccaccgccg tcgcacgcct
     2461 tttgagatcc tcgctgatgt tgaccccaca ctctccggcg gcggtcatat tgaaagcgat
     2521 gtcgaatcga ggcgacgcga tatcaccgga caggctttcc ccggcgagcg cccgacgcgc
     2581 cgcgttcgcc gccgcccggc cgatgtcccc gaaatcgacg tagaccgccg cgacggagcc
     2641 gtagtcgagg aaacgtttaa cggatgaata gacgggaagt ttgttcgccc aacaaaattc
     2701 cttgatcatc ctcacgtttt gttcgttcag gagcaacggg tccgccatga gccaaacggc
     2761 atccacgcgc ccctccagcc cccgcaggac atcgggcaat tcatccgacg acgtcacctc
     2821 caccggcagc accctcaccc ccgactcgtc cactctccgg cttttcgcca accccgaaag
     2881 gtgcgcgctc ccccacaaaa ccgccagtcg ttttaaattc ggctgaatct ccttgagttc
     2941 caggatgatc gtttgaaaag aagtctccat ggaaaccatc acggtttgtc cttttctttg
     3001 ggcgggatca atccaggtgc cgggggccag acaatagatc agcgtcgtcc cctccggata
     3061 gttttccatc gccgccttgc cgccgaaagc caccacgact tttgcggacg acggcaagga
     3121 cggcgcgccg tgggataaat caaaaacagc aacacccccc ggacccattc cctcccgaaa
     3181 cccttccagc gccgcggcgt aaggccccgt gttggacgcc acgaccgccg ccaccgaggc
     3241 ctcgtccgcg gcggccgaaa cagccagcgt cgccgccaga cagagagaga gcgcggaacc
     3301 gatgccgttt ttccgtttca tggcctgaat ttatactcca cgccgacttg gaaatttcgc
     3361 ccgtcctgcg ggatcgtcgc ctgaacgagc cccccatcgc ccgaagcaag ggaactggta
     3421 ttcgtcaggt tataaacgcc gagggtaaac ctcggaccgt cctcccacgg cgacaaggcc
     3481 gcattcagcg agatcacaac gtcccccggc actcgggtgt tctggtagct ggttcgacga
     3541 cccacataga aagtttccaa ccccagactc gcccgccacg ttgaaaaccg ccgggacaaa
     3601 ccggcgacgc cgctgtggcg gggcgagttg ctgagccggc cgccgccgat ttcgcgcgtg
     3661 ctttgtccga tgtatcccag acgggcggac aaacgggacg agacatccca gcgggcctcg
     3721 aattcgcctc ccttcgaaag cacctcctcc atattctgaa acaccaaact gccgtccgac
     3781 gggtccaccg tctgagagat caagtcgtgc acgtcgctgc gatagtaacc cgcgctcaat
     3841 tgcggcccgc ccccggccag ccgccgctcc cagcgggcct cataggtggt tatcctctcc
     3901 ggcctcagct gggggttcct tcgatagccg atgccgtcca ggaacatctc gtacaccgag
     3961 ggcgctcgaa aagcccgccc ataaaggagt ttaaaggagg acctccggtt cggctgaacg
     4021 atcaccgccg cccgcgggtt gacgctcttg ccgaaagact gatagtggtc gtagcggccg
     4081 cccgcggtga ggctcagaaa gcgcgcgggc ttcagttcct gctgaaagaa gacggcccat
     4141 cgccggagcg cggcatgaac gtccgtgtaa agggcatagg gttccacgtc gtggttcctc
     4201 aacaaaccct ccacgttttt ctcgaactcc tgaccgacga ggaacacgtt ctcctccccg
     4261 gcgacgttga cgctcaggcg ggtttcctcc ccataccatt cggattcggc caggtcccgg
     4321 ttgaccaggc gggtctccgg aaagggaccg tcgtacacat agtcggcgac ataacggtaa
     4381 tggtcggcgt acacccggcc atagacgttc accgcccccc ccaacggacg ctccagtttc
     4441 gtctccagaa aacgccgggc atccacggtc cagctgccgg gatcgttgaa ccgggtcccg
     4501 aaggacgcgg tgggaatgcg tttccggcgg ccgttgctgt tgccctggat ggacaatccg
     4561 tcccgggtaa acgacgcgaa acccgtgtaa gactcctcgc gatcggcgtt ttcggcaatc
     4621 ccgccgttga tcgccccgaa ttccggatac gacaggtcct gtccctccat ccagcggtag
     4681 gacccggcgg cgaaaatccc ccctcgggga ccgacgggct gggtccagga aaaaaattcc
     4741 cggttcgtcc cgtagctgcc cccgctcgcg tccacgaaaa cttccgggga cgcggaaggc
     4801 cttttggtga aaacgttcac cacggcgaaa aaagcgctgt cgccgtagag cgccgacccc
     4861 ggtcccttca ccacctcgat ccggtccacc gcccggatgt ccaggttgaa atcgtgtccg
     4921 aggtaggcgc cgccataact gttttcgttg atccgatgcc cgttgatcat gaccagcacg
     4981 cgggaattgt agtcgccggg acggccaaac ccgcgtaccc acaaataggt gtagttccgg
     5041 tcgttgatga cgtaaaaacc ggggatactc tggagcgcct cccccaacgt gcggtacccg
     5101 taaagagaga tctcttccgc ggagaggaca taggccgcgg ccggaacgcg agagattttc
     5161 tgcgggtgtt tcgccgcggt gaccacctcg gcctcgctct tgaaaaaatc aaagacctca
     5221 tcgctcggat aggcgttccc cgccgcaaag aagaccgcga tgaaaaaagt tcctattcgc
     5281 cgtttcacgg gatacgctcc cctcaggccg gaaggaatgc cctcagtttc ttttccagat
     5341 cttcgacgcc gaacggtttc ccgaggaaat cgtccgcgcc cgccgccaac atccgcttct
     5401 tcgtctgatc cacgttgata ccgctgatgg ccaacacctt cacgcccttt aggtcgtcgt
     5461 cggcccggat catccggcaa accctttcgc cgtccagccc gggaaggagc acgtccaaca
     5521 ccaccaaggc cggccgcaat tccgtcatct ttttcccagc ctcgaaaccg tccacggcct
     5581 cctccgccgc cacctcgctc ccatacacgt tccggagcac cctcttgatg aacttccgca
     5641 cgctctcctc gtcgtccacg atcaggatgg tcggcggttt gacgcccaac tcttccggaa
     5701 caggcatttt gagggatttc aggaaaacgg ccaaatcact gtcccacaca cggcgatgcc
     5761 cgccggccgt cacaaaggac ggaattttcc cctcctcgat ccatcgaccc accgtggggg
     5821 gggtcacgtg gcagaggcga gcgatgtcgt gcgtcccata ggccttcttt tccatggcga
     5881 aattctcctt tgtttcgttt tttacttatt ttatgtttaa tacctatttt ctcttttgtc
     5941 aaggggcatc ttttgacccc ctgaaacacc ttcatttctc aatatgtttc gaatgtaaat
     6001 ttttaataac cccctcggtg acacaagggc cctctgcgac cgcgggacaa cgtcccggtt
     6061 tgtcggacgg ggcacgtgtt cggtggcggg aaccgaagga agtcgagaag ggacttcccg
     6121 tcaagggaat ggttttattc gcggagaggg atgaaacggc gtgtgtttat agaggaggga
     6181 aacaactaag tagctattga aaccggcaaa ggcgacggag ttccgcggcg gtcaggggac
     6241 ttccacgatc tccccctggc gcagaaaaat gagctggaaa ccgcactccc ggccggtggc
     6301 ccgccgcacc gcctctcggt agaccgtggc ctgaggccgg tatctctcca atcgggaggg
     6361 aatatcctgt ggggagatcc gatccgtctt gtagtcggcc acccaaaacc ggccgttttc
     6421 ctgatagatc aaatcgatgc ttcctctcat gatgcttcca tcggatgaga aaatgaacgg
     6481 caattccttc cccaccacct cgccggcggc caacctcttc cccgcgggag agcccaaaaa
     6541 agaggccagc agcgtttggc tttctttttc gacgcggtcc cagtcggaca ccggatgatg
     6601 ccgccgcaac cggcggcagg cctggcgtgt cagcgccggg aggtcgcctc cccgccgata
     6661 atcccacgcc tccagaacgc ggtggcatac ctcgcccacc agcgccgcca cgttctccga
     6721 caggaacgcc tcccccgcct cgaacaccgg cttctcgcgt tcgtggggag agcgggtggg
     6781 cgagtcgaac agcacgcggt cttgccagcg ggagaactcg tcgtaacgcc ggcgccacac
     6841 aacggccagg tctttcgctt ccacgatccg cggcgcgccg cggatcttct ttttgtagat
     6901 cttctcctcc cgctccttgc gtatgttcca aacggggaac cgccccccgt cgccgaacac
     6961 cgcctccgcc gcgtcgtccg ccggccaagc ggcggcgtcc ttcaacatcc ggccgaacga
     7021 gctccgttgc cccccttcgg cgcggcccag caggatcagc ttctccttgg cccgggtgag
     7081 cgccacgtag aaaagacgca cccattccgc cgcctcccgg actttttcgt cccgttccaa
     7141 aatcgccatg gcgatgtccg cggatttggc cgacacgagc cggaaccccg ccaccccttc
     7201 cccccaatcc atacggaaag gcggccggcc ctctcccccg ccggtcacgt cggcgctgat
     7261 gttcggcaaa aaaaccacgg gatattccaa ccccttggcc ttgtggatcg tcaacaaccg
     7321 caccgcgtcc aaatgttcgt ccgccagagg gctctccccc tcctccgccg tctcttccat
     7381 ggaccgcacc acgaactcga tgaactcctt cagggtgacg ccgcggctct cgcccgcttc
     7441 cgccgccaac cgcccgaatt tctgcaggtt ggagaccgtc tgttcgccgt gataagccac
     7501 cgaggccagt tccaaaagga acgattcctt caaaagtcgg gagatgaaaa cacccaacgg
     7561 ttcccgcccc acccgcgccc gcgtctttcg gagaaaatca aagaacgccg ccaaccgggc
     7621 gcgcgcgccc gccgacaacg ccgcctcttc cggcgggtcc tgcaaatacg tgaacgcgtc
     7681 caacccctcc ggatcgccct cgtcggtggg cctgggaacg gccggtttcc gggcgaatcg
     7741 aagccggtag gtgtcctgat cgtccaaggc caccaacggc gagcgcagga gccccaggaa
     7801 cgagacccgg tcgtgcgggt cgtccagcac ccgcagcagg ttgacgaaat cgatcacttc
     7861 ctgagtgccg taaaagtacc ggtcgctctc caccacgtaa gggatgtggc tctccttgaa
     7921 aacgtccaaa tacacgttca acggcgtggt ggtgcgcagc agaacggcca cgtcgcggag
     7981 acggtagcgc ggcgccggcc cgtccgcggc ggcgaggcag ttcttctgga tccagcgcgt
     8041 catccagcgc gcctccatct gctggctgtc cgccgacgat aaaacgccgt cgtctcccgg
     8101 ccgcgtcaac accagctcca acgccgcctc cggcgtcttg ccctcgcctc gcctcccggc
     8161 ggccaaggtc ttttcctcgt tttccgggca gggatagatc ggcagatacg cgtgctgcag
     8221 gccgggattt ttcgggtctt cccgcatcac cgcggaacaa acggcgttca ccggacggat
     8281 caatgattca agacttcgga aattggtctg caaatcgcat ttccggcccc cctgggccag
     8341 gatgctctcg gtgaacctct tgtaggcgcg gatgtccgcc ccgcggaaac ggtagatgga
     8401 ctgcttgggg tcgcccacca cgaaaatctt cccgggcgag tagcggatgt tctcccacga
     8461 cgacgcgctg tctccggccg tttccgccag atacaacaag aactcgccct gcaccgggtc
     8521 ggtgtcctgg aactcgtcga tcaagatcgt ctcgaaccgc gccttaagcg cctcgcgcac
     8581 ctcgggccgg tcccgcacca gcgcccgcgc cgtcaacagc aaaccgtcga aggtcacgtg
     8641 gcccttgcgg gtgtatcgcc cgcggaacgc gccggcgaag ggccgcacca agtccaccac
     8701 tttgcgcacc aggctctccg ccaccgcgga cgtctgcatc accaccgcca ccccctcctg
     8761 atagagcgcc tcctccgccg ccggggtcca gctcttgggc cacttgcggc ttttctcctt
     8821 tgagaaatcg cggtaggtcc gctcggcggg gagcggcgcc ttcacgccgt aggaggcggc
     8881 caggatcctc agttcctgcg aaatcttcgc cagggattcc ggcatcttgc tgttggcgcc
     8941 ggggtcccgc tggccgacgg ccaaggcccc cacgtcgcgg gaaagacttt caatgcgttc
     9001 cttcacccgg gaggaaatct ccaacggaat ctcctccgac gcgtcgcggc aaagcgcccg
     9061 ggccaccagg gcgatgtcct ccaaggatgt cagcggaagg acttccagcc acaggccggc
     9121 gcggggcgct tcggtcccca gttcccgatc caaccaagcc gcccactcgc tttgaaagaa
     9181 ctcgtcgaag aactcgccct tgtccacctt gaagttcggg tccaccccgg cctcttcggg
     9241 atagagccgc agcacgcggg aagcgaagtg gtggatggtg ccgatctccg cccggtccat
     9301 ctcgtgaagg gccttctccg ccagttcccg cacgctcgcc ggcggccggc cgaaatgggt
     9361 cttcagttcc tccagaagtc cggcggcctt ccgggtccgt tcttcggtcg ggagatccct
     9421 tccctccaag acagccacca ggtcgttgag gcgcgccgag aggcgcactt tgatctcccc
     9481 cgccgccttt tccgtgaagg tcagcgccac gatgcgcggg agggcgatcc ccttcttctc
     9541 cggcccgccg gccaaaagga ggaacaggag ccggtcggtg agcagcgtgg tctttccggt
     9601 cccggcgccg gcctccacca ccacgttttc gtggagatgc cgccgggcga tattccgagc
     9661 ctccgcgtcc ttcacgattc ctccgcctcc gccggcgttt ttttcttttt gggttccacc
     9721 gacttcagtt tttcgtattc ttcccgcagc accgactcct cggcccggcg gcgcgtgggc
     9781 ggatgggact tgcggcacgc cgccccgaac gggcaatgcg tgcaatggcc gccctgcccc
     9841 tccttgggaa cgatgaagaa ttccccccgt tccatctgtt gcacgaaccc cgccaggttc
     9901 ttcagcaacg cggaccggtg cgccttccac acctccgccg gaaattcctg caccggctcg
     9961 gcgccggtca gggacgggga atcttccata atgtagtagc acacgccgga gggagacagc
    10021 ccgcgcacca ggccggattt ggggtgggac accatctcca gatacaccgg cggctggtgc
    10081 gcctgcgacg aaaggacgta ggacgcgagg gatttcttcc accccggcca gcgggtctta
    10141 taatcgatga tgcggaattt cttctccgag acctgcacgt ccacccggtc gggctggccg
    10201 cggaattcca atttctccat gggcccggtc aactccagcg gcgccggcac ccgcagctcc
    10261 ttctccatca ccgatggcac gtatccgctc tccgtcagtt ccgccatgtc ccgctccaac
    10321 acccgccgca cgttcaccgc catctggcgc cgcaccgact cccagaacag cggataggtc
    10381 ccggtccctt cccagctgaa ctcccggaaa accgactcca tcacttcttt caagaggggc
    10441 acccaatcgc cggcggccga cgagggtttt ttccaatatc cctgcgccgc gattttttca
    10501 tggaaccgtt gcatcagaag atggtagatc ctcccccgca cggtgcgggc caggtcgctg
    10561 cggtccgtgg cctcgtcgat ctcgcgcaag cccagcaccc ggctggcata aaactggaac
    10621 gggcacgtcc cgaaaacgtc catggccgtg ggcgagagcc cgcgcgtcag gagcccctcc
    10681 accacgccct ccgtgtggcc cacgatgccg tcgagcgcgt gcggttcgcc gaaccggttc
    10741 atgtccgtca cccggtcgag gcagtccttc aacagcttcg agtccgtgcc cagcggctcc
    10801 aacagcggcc ccgggtcctc cccgtcgcgc gccaacagca gggcggcctc cttgggagaa
    10861 aggaaggagg gcggcagggc gtccagcttg gcccacggct ggcgaggcac gcgttcgccg
    10921 ggatcgctct ccaggtcccg gcccgcggcg cggcacaact cccgcagata gatggacggc
    10981 acttgcgcct tgcccgaatc gtccgaccgg gcgtacagac aatagagctt ctcccgtgcc
    11041 gacgatacca tcaaatagaa aagaagtttc tcctcgtcat acccgcccag gcggggcgag
    11101 atccagtagc ctcccggctg ctgcagcagc gcccgcaggg aatccctcag cagcgggtcc
    11161 tcgcgcacct gacgggggaa gaacccttcc cgcaagccga tgaggaagag gacgcggaac
    11221 gaatcgcccc gggcgtccat ggcgttgaac acgcgcacgc cccggcggcc ctccggcggc
    11281 tccaaccccg cgcgcatcag tttttcctcc aacgtctcga ggaagacgtc ccagccggcg
    11341 ttcgggtcca acatgtcgaa cgcctttaaa tcctcaaaaa cctccagcgt ggcggcccag
    11401 gccggggcgt cgggatcgcc ggcctccact ttaaaattct cctccaacag ggcgcgggcg
    11461 taatcggcca ggcgcgtcca gccctgcaac tccgtcggcg ccaatcggcg gtgcaggtcc
    11521 tccagaaaat tccacagcgc ggcggtgtcc tcccgggcga tgcgataccc cggccggcct
    11581 tcggccgcca actgggggaa caattcgaaa tccttcttcg cccacggcgc cagcttgccc
    11641 cgccactgca accagcccga atgcaccccc agccggtcgg ccaactgttt ccaatggtcc
    11701 agcaggcggc cgccgcgcac gcgcccctgg aaccggtcga gacggaaata gggcgactcc
    11761 agcacatcca acaccgcgcc ggccggataa tcccgccggc ggagcgtcag gaacaaaagc
    11821 gtcagtttgg cgatggggtg catccggagg ggttccgact cccgcagaga gaacgggatg
    11881 ccgttctccg ccaacacctc ggccagcacg ccgcgatagg ggtccatgga ccgggccacg
    11941 atgccgatat cgtcgaactc cacgggattt ttcggccgct cccgcagcgc caggatctct
    12001 ttggccacgc gccagacctc gtcccgcaga cccgaaacgt tgatgatctt cagtttctcc
    12061 tccggcaccg tcaccgcggg ctgaacggga tcgaagagtc cgtccaacgc ccgccccaac
    12121 gccggcggcc cctcacaagg agcccccccc ggaagccgag gccggctctt tttcttcgcc
    12181 gaaccgtccg gcgaacggcc gcgcagatgg atcggctcgg ccccgccgac cggaagtttc
    12241 agttggtaaa accgctcggc gaaggaaaaa gccgggtgat ccttgcggta ggggaaaaaa
    12301 accgtggccg gatacatacg caccacgctc gaaaaaaaat ccgcctgggt gccgttgagg
    12361 ttatagaaac cgtaatagag gaattccccg tatttttcca gcgccgacga tttcccttcc
    12421 tccaacagcc gcgtggccac gcgttccaag cccgccaccg gcagcaccga catctcctcc
    12481 aaccgttcca ggtatttctc ctgcagcgtc aagaggaact ccagccgccg gccgaggtcg
    12541 cccgtcttca acacatcgct gaaatgctcc cggaacaagg ccggttccac gcccgcctcg
    12601 gccaagtcgc gaatgctggc ccgatacgcg gccgacaacc ccggcgacaa acggcgcgtc
    12661 tcccgccccg ccgcgcgcaa cagcgaatcc accagcttat cgtggaacaa ttcgtccccc
    12721 accaccacgc ggtcgggctc ggctgaaccg tcgagcacct ccatggccaa cgtgaaaaac
    12781 gtatggaacc ggatgttcaa caggggcagg ttctgttccc gagcgatgag ccgctgaagc
    12841 cgttcggaca tccgccggga cgggctcacc accgccacca agcggccggg gccgggcgga
    12901 tggagggaga gcgtatccat gagtgttttt tccaggcagg gctgaaacgg ggcgcagatg
    12961 atggcgggcg gcataagggt tcggcaaact cctcggcgga cgggtctatg aactcaattc
    13021 atgaaaaaga ctgggcgggg tggggatcga acccacatgg gccttgcggc ccccaggatt
    13081 ttaagtcctg tgcgtctgcc atttcgccac ccgcccgctc cgactgctat tttttcaggc
    13141 catgccccgc ctgaaaaaat tcccaaaatc tttcggagcc ggctgcactt gcgatttatg
    13201 caatgtgcgc ccacttattt cgaaatgggc tgtccctact cgggcgagca aggacgcccc
    13261 agataaaatg cccaaaaacg actgcggaac gggctgtcct catgcggggc atgggagcgc
    13321 ccaataaacc agcgttgctc gcggaccgtc ctttcgcatc aagcggatgg cagttttcgt
    13381 tgcatccgct taagttccgc aaaagggcgc ccattccaaa aatttatcgt ggaagggacg
    13441 gacgcctgtc gtgagacagg ggctcccaaa atcgtcaagc gattttagca aatacggatg
    13501 gatttactca ggaagcggcg tggagaaaaa cttcgacggt tgactacaaa tttttgacga
    13561 acttgttctt tttatccacc aaaatgttct tgggaacaat gggctgatcg gtcatttcga
    13621 agcccatgat gatgatctcc tcccccacct tgatcaaatg ggcggcggcg ccgttcatgc
    13681 agatcgtgcc cgaattcctt ttgcccacga tcacataggt ttccagacgc gccccggtgg
    13741 tgttgctggc caccagcacc ttctcccccg gccaaatgcc cgtccgttcc accaaatcct
    13801 catcaatggt gatgctcccg acataattca cgtcggcttc cgtcacgatc gccttatgga
    13861 tttttgagcg cagctgccat ctcatgggat gcttcgtcga cccctctcgg aaaaatttcg
    13921 cggtgatatt ctggcaatat cccggcaata agtcaaaacc atcctcggcc tcgcccgttc
    13981 ctcgtgccct cggaattttc cattcagcct tgcgggttgc cggggacgta ttccgtcatg
    14041 tcggcctgaa aagcgcccac cgccacttcc gactgcagca acacgggcac cttgcgttcg
    14101 tcccgcgtga accagaccac caggcggccc ttgcttttga agagcccctc tcccgccagc
    14161 gtgggctcca ccctccaaca ttcgaatttc cccgccggcg tctgcacggt ctccaccccc
    14221 tgcaccgtca gtttcagcgg ccagttgcgt ccgccggagt tgacgtcaaa aattatctcc
    14281 gaacccaccg tccattcgcg cgtgcgcgcg taatacaggc tcgacaagat gtcctgggtg
    14341 aacgggggga agggctgacc caggcggctg tcgaacggat ggtctttgaa cacataactg
    14401 aaaatgcccg agggatggtc gaacgcggtg gcggtttctt tgtaatagcc tccctcgcgg
    14461 acgctctgga cgaaccccag agagcagaga ctctccgcgt ccatccagga ctcgttgaga
    14521 tcgcgcacct tgaacaccct gtccaacatt ttgttggtgc tggcgcggga gacgatgcgg
    14581 taggcaaggc gaccggccag gcgctgggga ccgccaacct ccatcagcac cgagccgccc
    14641 gtcatcatcc cgtaacggat gcgatagctg atcttctcgc cggccccaaa agcgcggttc
    14701 gtttcacgcc gccacgcaaa agccggggcg tcggcgcgca aaggaacaaa agcctggagc
    14761 gccagcaccg cgaggagtcc ggcgacgctt ttcacgcgtc tgaaacgggg cgggtggatc
    14821 gtcatttttt tcgatggatg aatctcacgt agtccatgtc ggattgcctg atgtgattga
    14881 cgatccaacc ggaaaacaac cggctcactt tcatggcgat ccacatgtcg ccgccccgct
    14941 tgaactgcgc gacgagatca ttgagggatt gagtgatgtc cgtgtgttgc caggaatggg
    15001 cgctgagctt tggatatgcg tgcgcccgca tgaatttctc ttccgcatgg aaatgccttt
    15061 tcacgtagcc catcaagaac tggagggcct cgcccacggc cttgtcgttt ttcccccgcc
    15121 cgacggcctc gaacaacctg ttggcccgtt cgaagagctc gcggtgttgc ccatcgatca
    15181 attcaatgtc ggtccgaagc gagtcgtccc agacgatggc cggcacagaa agcccctcac
    15241 gtccgaccgg ctaaccggcc agacacgtga cgatcccgcc cagatacatg cggtgatagt
    15301 ccttcagcgg ataaaaagcg gcgatcttcg ggtcccggaa attcttcggg tcgaaatcct
    15361 cgaagtagat cttttcgcag atcacgacca gccgcgcttc cttgaaatac accgtcccga
    15421 attcctccac cggcgtcagt ccgctaccgg cgatcttgtc ggtgtcccgg ccggagttgc
    15481 tcccgcagta gttcagcgcg gaacgatatt tctcatcgaa gaacgaaagg gagaaccggt
    15541 cgtttttctc cagaaattga tacgtgtgcc gcacgggccg cacaaaacaa aaacagacgt
    15601 tcttgtccca cagaacgccg aaaccgcccc agctggccgt catggtgttg cacgacttct
    15661 tcgtgccggc ggtgatgagc gtccaatccg acccgaaaag cttgaagggg ttgacccgga
    15721 tgtcggcggg agagatctct ttcatgacct atctcccgcc acggagaggg ctggatccga
    15781 gatcgcggac cacctggttg actttcagat ccccgcgcgg ctcctccatg tccaacggaa
    15841 ggatcacctc gtttttgagc aggctcgccc ccgccacccc ctcgaaggcc tggcggaaca
    15901 tccagccggc ggcgccggta tagccgtgcc agatcatgcg gccctgatcg aacgtggtga
    15961 gcatgtccgc ggcctgtttg ttgggctggc cgccgtagag ttcgatctgg tccggagtca
    16021 cgtggtccaa cggcgatatt ttccgccaga gccggtaggc ggcggcgcga aattccttgg
    16081 ccttggcggc ctcgccctgc tgcgcgaaac gttccgccag aatgcgcgcg gccttgacca
    16141 gccattgaac cccgtggctg tacatgccgt tctcccgcac gccttcgggg tagtgactgc
    16201 tccgtcccaa aaagggcttg gtgtcttccc gcagggccgg ccagccgagg aggatgacgt
    16261 tctctttctc cagcacgcgc agggcggttt ggaacacggt gaggctgcgt tccgggttga
    16321 tgccggccat caccgcccac gccgcggtga gggcgtcgat ctcccacacg ccgctgtctt
    16381 tgacgccgat ctcggtgccg tcgtcgtgga tggcgcgcag ataccggtcc tcccgccacg
    16441 tcttctccaa ggactcttcg agacttttca gacggacggc atagtgttcc ttgcgtttgg
    16501 ggccgtcctt cttctcgatg agggggagca tggctttcag gatgtagtcg aggaaaaatc
    16561 ccaaccacac gctttccccc cgcccctggc tgccgatctc gtccaagccg tcgttccaat
    16621 cgccggtgcc gatgagcggg aggccgtgcg cccccatgcg cttgtccaga accaggtcga
    16681 tggccttcat gcagtgccgg tacacggtgt cttctcgggt ggaacgctgg tagacggacc
    16741 cccagccgtg tttcatcttg gggagcgcct ggaacgggtt ctctgaacgg agataggagg
    16801 tcatctcctc gaggagggtc tcatccccgg tggtccggac gtattccccc acggcccagg
    16861 ggagccagag gagattgtcg gaggcgtgag accggttgga aaacgcggtg cggccgtcgt
    16921 ggagcgtgtg gaaccagtgg accacgtctc cctcgatgaa ctgctgcgag gcgtgcaaaa
    16981 taatttgttt tcgcgccaag gcgggatcca cccagacgag gttgacggaa tcctgaagct
    17041 ggtcgcggaa accgaaggcc ccgctggtct gatagaaacc gcggcgggcc cagaggcgtt
    17101 cggcgatgcc ctgatatttg agccagttga ggagctggtc gaactccggc tggttggagc
    17161 ggatcttgac cgtttccatg aggctgagcc accacttgcg cgtctccagc aaacgggcgc
    17221 gggcggactc gaccgtcttg tatttctgta tcagcgcggc ggcctgtttg cggtcatcca
    17281 cctgccccag gagaacgacc acggtgcgct cgcttttggc ggggatgtcc accgtggcca
    17341 ggaagccggc gatgggccgg tcctcgtcca accgcaggtc ggccggcacg cccttttcca
    17401 ccataaaggg atggtccgga ccgcggcccg gcccgaagaa tcggccgcgt tcggtctcca
    17461 cccggtccga cgggacggag agggcggcga aagcggggcc ggtgcggaac gagttgcggg
    17521 gattttcgaa aaagacggcg ttgagggcgc ggtcgtgggc gacctgaagc ggaccgtggc
    17581 ggcgttccgc ctggccggtg agcaccacct ggaaatacgg ggcgagacgg aaacgccgcg
    17641 tcttgtccga attgttcttg atggtcaaca gatacacgcc ggtcgaatcc gccgggggca
    17701 cgaacaccgt gagttccgtc gagaccgtcc cccgggtcat gcggaagagg gcggtgccgt
    17761 ccacgccgaa ctcgcattcg tgtttggcgg aatggtcgtt gagcgggtgg agcgtgggac
    17821 agtaccactc gttctcgtcc cgatcgaaca gatagatggc ctcggcggat acctcgcgcg
    17881 tgacggtgtc gggccaatcg ggcgtgatgg ggttctgctg ggagttgccg ttcatggagg
    17941 tatgcagacc gcggttggtg accatgacgg tgtgcccgat ggcgttggac atggcgtggt
    18001 cgtacggccg gggcgtgtac ggcgttcgga cgaacagctt gttgccgttc tccgtgaact
    18061 ccgaataggg ctggggcgtg ccgggcagga tctccccgtg accgatcaag ggggccttcg
    18121 cgccttttcg ggacgcctcg cgcgcgacgg cctttcgttc ggaggacggc gtgggacgga
    18181 gatatttttt gatgaacgcc agggcgttct cccggttttt ggcgcagccg atcatcaggc
    18241 gcagggactc ggcggatttc ggcggcagcg cgacgggcag agacaggctg gcgatgggat
    18301 cgaaggtggg ataggccgcg gcggcccggg cctccagaaa acccaacgtt tccagcacgc
    18361 gcggcgtcca gaggctgcgg gcgcggccga tgaaatccat ccgcgaggag aggaatccct
    18421 ccggcgaccg ttccgacgcc aacaggccca tggacttgga atgtttggac caggccagca
    18481 cggcgttgac gtcggcggcg tattccatct ccggaaacag acggttgtat tgtgtgtggc
    18541 ccttgtccgc gtcgggtcgg tccagcaccc actcgagata agggaccacc ttgagctggc
    18601 gcggcgcgtc cgtgaggttc tcaagcgtca cgtccacgat ctccgccgga acgtcggggt
    18661 ccggcaggga catcgtcacc gtggtgcgga tgccgttgtg ggtgttgacc acgcggaggg
    18721 cgtcctcggc ctgttccacg cgggacggtt cgaatttttc tttggggaaa ttccccatca
    18781 ccggccagga ccgggagggg ttgccgggtt ttgagaccgt atccaccacg aagagggcgc
    18841 ggccgcaggg atgcaggggg tcgtaggacc gccgggtgag gtcgtatccc ttggaaagat
    18901 attcgctgtg tccctcgccg ttctcaccga tcgccagccg gtattcccgg ttgaccagct
    18961 catagacctt ctcgcgccgg agcgcggccc gacgacggaa cgagcgcagg acgaaagaaa
    19021 ccaccgtgcc cgccgccacc gccgccagcg cgaggacggc ccactgagac agcgtcaacg
    19081 cccgcgccgc cgaaaccagc ccaccgccct gagcggcgtt ctggatttcc tgcgatttgt
    19141 tccaggcgta atcccaggcc tctccgcgcg ggatgtagaa aggaatgagc aggggcgccc
    19201 aggtggtgaa ccgcttaaca ccttccggga tgtattgctg ggcggccgac cggccgatga
    19261 accgcgccac gcgttcgtcc agcttgttca tgccgatgaa actcaggttc accaacgtgg
    19321 ccacgcgcag ccccatgtgg tcgagccaaa tgagaacccg caccaggtcg tatgagagaa
    19381 gcgccacgaa aacctgaagg ctccaggcct gaaaatccga cggactgagc gtgatgttgt
    19441 gatacacggc caccaaggtg cgaagggcgc cgtcttggtt gtaccaggtg gccttggcca
    19501 tcatgcgcag gaaggtgttg atgatgggcg acatccacaa accccaccgg aggacgtgga
    19561 tcatgttcat ggtgagctcc acgaaccccg ccttggagaa gaaatgtttg atgggcgcgt
    19621 tgtctttctg gaaatacgcc tccatgaagg tgcggttgac ggcgaagagc caggcggcga
    19681 tggcccagcc gatgaccccc gcgagggctt cgttgtagag cagtttgacg ccgccgctga
    19741 agtggcccag gtcgacccgg ccccacttgc tgagccacgg atagatggtg tagtccttgg
    19801 gctccagccc ggcgctgttg taaagcttga atttttcgac gatcacgggg acctgggagg
    19861 aatccagata gaacgccatg gcgctgccga tgaacccgcc catgagcgca tcgatgaagt
    19921 agagccgcca cgactggagc cgccctttcc gacggacggc gttcacggcg tcccggacga
    19981 cgctcacccc caccgaggcc aggaggccga tggccagacc gaaccacagc cgctccgaca
    20041 tctcccgctg gaacagcccc tgcgtgaacc cataagccag accggcgccg gtcacc
//
LOCUS       DAOTFD010000022        19658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1815_length_19658_cov_4.655614, whole genome shotgun sequence.
ACCESSION   DAOTFD010000022 DAOTFD010000000
VERSION     DAOTFD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 19658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19658
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_19658_cov_4.655614"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..601)
                     /gene="nadD"
                     /locus_tag="P5079_02605"
     CDS             complement(20..601)
                     /gene="nadD"
                     /locus_tag="P5079_02605"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRY28909.1"
                     /translation="MKFGILGGTFDPVHRAHVALAEAALRELKLDRVFFVPAGRPPLK
                     NAGSVSPARHRLALLRAALRGRPKFRILLWELRRSGVSYTHHTVAYLRKRYPAAEGYL
                     LIGGDSLKNFTRWRRWTWLLRNIRVVVGRRPGVGLGGVPSNLRKRVIFLKKKLPAVSA
                     TEIRRRVRAGETLNGFVVPAVAGHVRRAGLYRR"
     gene            complement(643..1968)
                     /locus_tag="P5079_02610"
     CDS             complement(643..1968)
                     /locus_tag="P5079_02610"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRY28910.1"
                     /translation="MAKKKKPRKSVRGFRPVTLSTPADYREEIAGVCLKAKQASRLLA
                     LTSTAARNRALESIASALERRRDEILFKNEIDLEAGRKAGLSAALLDRLTLTADRVRA
                     MSQGLRDIAQLPDPLDETVSEWKRPNGLVVRKVRVPLGVVAMIYEARPNVTVDSAGLC
                     LKSGNAVVLRGGKEAFNSNTALVGVVQSALGETEISPAAVQFVSTTDRAAIRDLVRMD
                     KLVDLVIPRGGEEMVQAIRDMATVPVLSHGKGLCAVYVDKAADLDMAARIAFNAKVQR
                     PGVCNAMETLLVHRDVAGRFLPGMVGKYAAAGVEVHGDDETKRLGGKTVKAARSEDWD
                     TEFLNLTVAVKVVASLEEAVAHINMHGSHHSDSIVTDDAGAAEKFLSQVDSAAVFHNA
                     STRLHDGSVFGLGAEMGISTQKLHARGTMGLRELTTTKYVVRGSGQVRE"
     gene            2454..5144
                     /gene="acnA"
                     /locus_tag="P5079_02615"
     CDS             2454..5144
                     /gene="acnA"
                     /locus_tag="P5079_02615"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRY28911.1"
                     /translation="MENSFNSAGILTVNGKKHAIFRLSALEKAGLTKIHQLPFTVRIL
                     LENLLRHEDGRLVQKADVEALARWSPKSPPTREIPFIPARVLLQDFTGVPVVVDLAAM
                     RDAAKKFGVDPGRINPLFPVDLVIDHSLQVEFFGSPDAFQKNVKLEYHRNRERYTFLR
                     WGQKTLSNFRAVPPGAGIVHQVNLEYLASVVCAKGDAVSPDTVIGTDSHTTMINGLGV
                     LGWGVGGIEAEAAMLGQPLTLLMPEVVGFKLTGRLPDGATATDAVLTVTEMLRKKNVV
                     GKFVEFFGEGLSALSLPDRTTIANMAPEYGATAGLFPVDEETLSFLRLTGRPDDHIAL
                     VEAYCKEQGLFRMPNDPAPLYSDTLELNLSAVVPSLAGPKRPHDRVPLAEAKQAFKKS
                     LSAPVKERGFELPPESLNKHVTLRMGERADEVGHGVVVIAAITSCTNTSNPSVMIAAG
                     LLAKKAVERGLSSLPYVKTSLAPGSQVVTDYLNAAGLTPYLEKLNFHLVGYGCTTCIG
                     NSGPLPEPVAAAVKEGNLVAAAVLSGNRNFEGRVNPLTRANYLASPPLVVAYALAGTV
                     DIDLSSEPIGTGKDGKPVFLRDLWPSNKEIREAIKKAVKPELFRRRYTRIADGDENWN
                     SLPVLEGNLYAWDEKSTYIKHPPYFRDFALDPAELKDIQGLRVLALLGDSVTTDHISP
                     AGNIAKDSPAAKYLISLGVEPKDFNSYGARRGNHEVMARGTFANVRLKNLLVPGTEGG
                     VTVHRPSGDTVSIFEASQRYLKEGVPLLVIAGKEYGSGSSRDWAAKGPMLLGVRAVIA
                     ESYERIHRSNLVGMGLLPLQFIPNESRESLRLTGRETFSITGIAEGLTPKKVLSVTAR
                     REDGSTFTFNAVARLDTPVEVDYYRHGGILQAVLRPLCRP"
     gene            complement(5230..6369)
                     /locus_tag="P5079_02620"
     CDS             complement(5230..6369)
                     /locus_tag="P5079_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRY28912.1"
                     /translation="MTPLQILAFVGLFVGLTVVGAAIFLVWQMMRASQNTRAADPGLQ
                     LMQQQLEGLRSQLAETLSGQTQNVNQQLHQVTTQVSQRLENISSQMMTSQKSVGERLD
                     NAARVVSEVQKSLGTLGQAAERIFDVGKDIAGLQEILRAPKMRGGLGEFFLGDLLSQI
                     LPTRHYELQHAFKNGGVVDAVVRLGGKLVPVDAKFPLENFRKVMDAKTDEERKPLKKK
                     LAGDVKKHIDDIADKYILPDEDTYDFALMYVPAENVYYEIIVKDEDSEEGKSLAEYAL
                     KRRVIPVSPNSFYAYLQAIILGLRGFEIEENAKVIMENLARLQGDFHRFREDFDVLGG
                     HVSKVKGKYDDAEKRLERFQEKLASIGHSTPDLPASSTPALPPKS"
     gene            complement(6382..7074)
                     /locus_tag="P5079_02625"
     CDS             complement(6382..7074)
                     /locus_tag="P5079_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28913.1"
                     /translation="MESNRNCFESINFNKKWDEEDWERFFSAQDEFVRQVRPGRAYAR
                     GRTVDPSLSFRNVMRRFGMDPDGVFAPEASFEPPAGPSTEPPLWKSRLSLEEAEVDAL
                     PIYIRAKSFVGVVASLWERRFQKMMAKTYKSRLHRLCQALLEDIRHHARRIPHNVAEG
                     HYVGYGAAAVKGNIARCRRALTHADACVGFVSRLPRRFMSAEEYHLLFRETLGLRNDL
                     MAWIFFLRQMNA"
     gene            complement(7111..7278)
                     /locus_tag="P5079_02630"
     CDS             complement(7111..7278)
                     /locus_tag="P5079_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRY28914.1"
                     /translation="MGLKEKLGIFRELLDYLWEQKLWWMIPMVSVLVLFGVLLFLAQG
                     SAVAPFIYTLF"
     gene            complement(7302..7712)
                     /locus_tag="P5079_02635"
     CDS             complement(7302..7712)
                     /locus_tag="P5079_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY28915.1"
                     /translation="MKTHTEYLWFNTRSRKELVNITADVAKYVKASGVKEGLCLVSAM
                     HITAAIWVNDEEDGLKQDVFDWLERLAPPADYRHHRTGEDNGDAHLKRTLLHHQVVLP
                     ITSGRLDLGPWEQIFYAEFDGQRKKRVVVKIIGE"
     gene            complement(7709..9022)
                     /locus_tag="P5079_02640"
     CDS             complement(7709..9022)
                     /locus_tag="P5079_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRY28916.1"
                     /translation="MSEPAISKNKANRWLGLWIPPLLWCGVIFYFSGIPDLSTGWGMW
                     DLVFRKAAHAAEFGILAALLWRALEHTFSWTLSRIFWVCVSFSILYAASDEWHQGFIP
                     GRVPSAWDAGIDALGALLVCRWQRRWGMNLFAKLSPFYTRPGRVALALSFLGVLGCGR
                     LGFFKAERLEKAGRFEEALRAYVAVADQVPSGAIAPKALYAAGRLAGRTLQDYELARG
                     LFQRLIDQHGDRKGWGDKAERAIFNTPNYFPLVVNGQWEEGDSDTGGRNAKIRIVCRV
                     ADDDPAAVLMTHNFYAGDKFQKNLSIRKRYKKEKLELREYLNDKSAPTVILKYPFEKG
                     SRWTSRRDGRNFIFTVDGMAAVKVRAGEFRDCLVVREQMEGITGSWQLNYYAPGVGHV
                     LTTLATSGGERRNTELLSYNLSGGHEAGEDKLGRSARVFKEKEKQ"
     gene            complement(9019..9696)
                     /gene="radC"
                     /locus_tag="P5079_02645"
     CDS             complement(9019..9696)
                     /gene="radC"
                     /locus_tag="P5079_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY28917.1"
                     /translation="MLTSASFNRLHRIEKPREKLARGGPGALKDEELLAILLRTGYKG
                     KDVLTLSRELVRKHPGKQLFELSFEELAKIKGVGDTRAATLLAAWELARRHSGSGDEA
                     ASVQTPKDVCQHLAEIRDRKKENFVVLYLDTRNRLAHKEFVSIGTLNASLVHPREVFE
                     PALRHSAASVILAHNHPSGDPTPSEEDRRLTKRLAQAGELLGIEVLDHVIVSAQKHHS
                     FREKGDL"
     gene            complement(9822..10484)
                     /locus_tag="P5079_02650"
     CDS             complement(9822..10484)
                     /locus_tag="P5079_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY28918.1"
                     /translation="MELQAFTGNFADNSTEAGFQFTFFCDSCKEGYKTKFTESKSHKT
                     AGLLRGLGQAAFTIANMLGKSGVGYGLERGADALREKYRGMSPEWHKEHEKAFELAQN
                     EAKGHFKRCPKCKKWVCETDWNEQEGLCVDDAPRMNVEVAAAKAKKMVKDIQSAADTK
                     QVFSGEIESKQTVCPQCGKPAGQGKFCNNCGADLSMLKCSKCGAQSPAGTRFCGNCGG
                     KL"
     gene            complement(10515..10853)
                     /locus_tag="P5079_02655"
     CDS             complement(10515..10853)
                     /locus_tag="P5079_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY28919.1"
                     /translation="MTGYKHPCRYCGKLIPPESKVCPLCGKINPLDALRCPKCRMPVE
                     KEYKNCANCGLKLEVACPKCGKATFFGDYCALCDARLAVICPNPKCKAEQPPLDGNCI
                     KCGKSLKQVQ"
     gene            complement(10907..12223)
                     /locus_tag="P5079_02660"
     CDS             complement(10907..12223)
                     /locus_tag="P5079_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28920.1"
                     /translation="MPEDKEEILFEAPLKYSLEEGPGEGAVSGDATLKLTPVGMFVRP
                     RRGEPLCLSYHDVLEYSAKDHAISLALSSAERLKLTDIGYGYDDLIRIFTGFRNEMLL
                     RDLLMKESTIKKDVKADLSGNGADGQVIEAKNVALRICSTALVLLFGDRDPVRIPFCD
                     ILETNEGDFKLEIKTELGEKYVLSGMGYEFGPFAKALSDRMGELALMVQTTLREIVPG
                     ADPKILRKAAGLMREGKAAKKSDLDAISPDIWRQLERKMALTELKEEYEFLRSKAQDT
                     RMGIGLKRDSSGEYLWFLAPIYSDDPRVPGNLIAMESVTEKETGKATYFYRIVSRKDY
                     RKSLKLEELHKEADNAIRNINRCMLAINFRREPIYLPPEKLAEPQYQKYEYAIAGVPG
                     LRMLRELYVGRVIHSSDGQWKEDVAELLKFNSEAEDGSRWCQKDKA"
     gene            12405..12674
                     /locus_tag="P5079_02665"
     CDS             12405..12674
                     /locus_tag="P5079_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28921.1"
                     /translation="MANGLLFMSRPCAVRNILPPMFMFEPSGVGPVSPSGTFPTNTKI
                     FANGPFARGFSNWGIRDLKLLQATKTANPAKTANIFVRFPIFILL"
     gene            complement(12940..>13664)
                     /locus_tag="P5079_02670"
     CDS             complement(12940..>13664)
                     /locus_tag="P5079_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28922.1"
                     /translation="AYFQLGNLYRDRGEYDRAKRMYVAGMKEAPRDLLGGYIELAELH
                     RMNGEIEEAKKIYEKMIKTFPENPYAYDSLGTLYLECQEYEKAEEVFNRGYLLTPEFE
                     QGLSMIYFLQGKTELAKKYGGANYRLPPNSLRGYRKLRDVVLGRGIKLVCMQYPRCPV
                     ADLEKVFGKKAGIRYVENVANFEKALETHEVKEIFRDLFFGNFGHCTDLGNRLIAENL
                     ADAILRGVFGDRDSVGRGAMLN"
     assembly_gap    13665..13674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13675..14451)
                     /locus_tag="P5079_02675"
     CDS             complement(<13675..14451)
                     /locus_tag="P5079_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28923.1"
                     /translation="MNDDFRAYIKFLGNAALKAGLAIIILFACVGIYNRSSLFLQERR
                     NLKGLSAEGGYKILCFGDSTTQAQWPPILEEELNRRGGNIRFKVIDRGRVFNRISRVL
                     YFLEGDLDKYQPDMVVVMMGMLEGRWGTKETGWGGWLADRFDSFWAGVRLLVGRWGEG
                     ERRNGERKLIKVFEWLERRPKEEAPPKEAASLKADDYIRWGKRYRGEGRFDKAEKMFK
                     QGIAASPGDASGYVLLGRLYQAKDDDEKAEELYRAGIRAVN"
     gene            complement(14470..15879)
                     /locus_tag="P5079_02680"
     CDS             complement(14470..15879)
                     /locus_tag="P5079_02680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRY28924.1"
                     /translation="MLFNSTVFLLFFFITLTLHWAIRKNTYRNLLLLVASYIFYGWWD
                     YRFLLLLFASSLVDYLCGLGLAKEGWQRPRTRKLILSVSLSVNLGLLLFFKYFNFFVE
                     SSAAFLRVLGFSPHMPALNIILPVGISFYTFQTMAYALDVYRYAIPATKEPLPYFLYV
                     AYFPQLVAGPIERAGNLMPQFMRERHFDMDMVQKGFRCMLWGYVLKCVVADNLAVVVD
                     GVFADLARCSGGQIVFGVYCFAFQIYGDFAGYSYIAMGASLFFGVRLTQNFRSPFFSE
                     SVRDFWKRWNISLMNWLRDYVYIFALGGRIKPVSRWAGNVLITFVASGLWHGAQWTFI
                     VFGLVNGVFYFMKKPFRQENAAARFLNMTATFHLFCCGLVFFRAATLSDALAAYRNVF
                     RSLGGASVLLPDHAFCGRAFFLMGLVVFVEWVQRKREDAVAIERVPAWGRFLIYLVFL
                     TLLFVFGNFNHAPFIYFQF"
     gene            complement(16116..16445)
                     /locus_tag="P5079_02685"
     CDS             complement(16116..16445)
                     /locus_tag="P5079_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HRY28925.1"
                     /translation="MRIWDLPPWRLCRAHLLGEHRELHAVWNIITRKKSGYSRHPETL
                     RWKGKLRALYGRHDELVEEMGRRGYEHRSPLRRELAAGAAVQKVLLASLREQRARLGK
                     KNCGCRV"
     gene            16662..18086
                     /locus_tag="P5079_02690"
     CDS             16662..18086
                     /locus_tag="P5079_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28926.1"
                     /translation="MKIVLIDPPFEGPDSLGNNVSLAPVVNVVPSMGLAYLAAVLEKA
                     GHAVKILDATLGLSSASVLDETVRFGPQIIGVTATTPNFRSARRLAQEIRRQLPEILL
                     VLGGAHVTCAPEHAMSFGLFNVGVVGEGEETLLELVKTYAARGAAGLESVPGLVFRRD
                     NDLIRTPPRPFIHRLDELPFPARHLQPPLAAYRPTPCTYRRLPLGVMITSRGCPSKCT
                     FCDRSIFGTKYRTRSPENVVAELSEVVRKHGAREIRFYDDAFTLDHRRVYTILDLMAK
                     EKLRPSWTCLTKARGISRDLLAAMKAGGCWQVQFGLESGDPEMLLRMGKGNTVEDNRR
                     AVSWARTLGLTVRGDFVVGTPGETAQSLERTFCHAVKLKLDYAYFHKFVPYPGSELYE
                     TLTARGHRFDFDTLGSSVVDNDSIHYVPDGLDAAHYRRWLNAVYKRFYFRFGYVLRRA
                     MAVRSWEEWRGQVRGFQAVAGLKT"
     gene            complement(18235..>19658)
                     /locus_tag="P5079_02695"
     CDS             complement(18235..>19658)
                     /locus_tag="P5079_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY28927.1"
                     /translation="VKTGLIASVLLSVFVFVMRTPLSAADAGTARSLLNSKKMNDRLD
                     GVSMLREVGNDEAVEVLIGLLEDKKDKIREASVAALGDISLPLASEGLSKALNDPEAN
                     IRVLAVQNIAKKSKADVIGRLEPALGDKDDAVRRESVKVLADLKSKDAVPVLSKAVSD
                     RQTDVRRTAVESLGTLADSACIPALTKAVDDPDKDIRVLAVSHLWKIGGSQTFPGLLR
                     VATNEKVLGLRLSAVNILGQIGDETVITDLSRALKDPDVSVRLAAVGSLGLLGGAPSS
                     LSLAVALNDKEVAVRESAAVVMGILAVPESLSPLTEAIQREMDPNVICALATAIGRVG
                     NKAAVPTLMQTAPRLKPPANEPIYQAIAALVEKNKPAPVAAKKEASPTPPPAPAPAAV
                     SVPVPAPPAAAQAMPSEEKLRLMQENYIKGQDYFMQKNYKESIRYLENVLKLEPNHPP
                     SKSLLARARRELKKSSAPPPAPK"
BASE COUNT         4107 a         6230 c         5247 g         4064 t
ORIGIN      
        1 tcctctgtcg attctttttt catcgccgat ataacccggc gcggcggacg tgcccggcca
       61 ccgccggaac cacgaacccg ttgagagttt ccccggcccg gacccgccgc cgaatctccg
      121 ttgccgacac cgccggcagc ttttttttca aaaaaatcac gcgctttctg aggttcgacg
      181 gaacaccgcc caatccgacg cccggacgcc gccccaccac cacgcggatg ttccgcaaca
      241 accaggtcca ccggcgccaa cgggtgaaat ttttcagcga gtcgccgccg atcaggaggt
      301 atccttcggc ggccggataa cgtttccgca gataagccac ggtgtgatgg gtataagaca
      361 cgccgctccg gcgcaactcc cacaaaagta tccgaaactt cggccggcca cgcagcgccg
      421 ctctcaacaa ggccaggcgg tgccgggcgg ggctcacgct tccggcattt ttcaacggcg
      481 gccggccggc cggcacgaag aaaacgcggt cgagtttcag ttcccggagg gccgcctcgg
      541 ccagggccac gtgcgcccgg tgcacgggat cgaacgtgcc tcccaaaata ccgaatttca
      601 tacggggata cgccgtggac gagcggtcgc tgcggaaatt ttttattccc tgacctggcc
      661 ggaaccacgg accacgtatt tggtggtggt cagttcccgc agacccatgg tgccgcgagc
      721 atgaagcttt tgggtggaga tgcccatctc ggcccccaaa ccgaacacgc tcccgtcgtg
      781 caaacgggtg gaggcgttat ggaacaccgc cgccgaatcc acctgggaaa gaaatttttc
      841 cgccgccccg gcgtcgtccg tcacgatgct gtccgaatga tggctgccgt gcatgttgat
      901 gtgcgcgacg gcctcctcca aagaagccac cactttcacc gccacggtca aattcaggaa
      961 ctccgtatcc caatcctcgg accgggccgc tttgacggtt ttcccaccca gccgtttcgt
     1021 ctcgtcgtcc ccgtgcacct ccaccccggc ggcggcgtat tttcccacca tcccgggcag
     1081 aaaccggccg gccacgtccc ggtgcaccaa aagagtttcc atggcgttgc acacgccggg
     1141 ccgctgcact ttggcgttga aagcgatgcg cgccgccatg tcgaggtcgg cggccttgtc
     1201 cacatacacc gcgcaaagcc ccttcccgtg gctgagcacc ggcaccgtgg ccatgtcgcg
     1261 gatggcctgc accatctcct cgccgccgcg ggggatcacc aaatccacca gtttatccat
     1321 gcgcaccagg tcccggatgg cggcgcggtc cgtggtgctc acgaactgca ccgcggccgg
     1381 agagatttcg gtctccccca aggcggactg caccacgccg accagcgcgg tgttggaatt
     1441 gaacgcctcc ttgccgccgc gcaacaccac cgcgttgccc gatttcaggc aaaggccggc
     1501 cgagtctacc gtcacgttgg gacgcgcctc atagatcatg gccaccacgc ccaacggcac
     1561 gcggaccttg cggacgacca gcccgttggg ccgtttccac tcgctcaccg tttcgtcgag
     1621 cggatcgggc agctgggcga tgtcgcgcag gccctgcgac atggcgcgga cgcggtccgc
     1681 cgtgagcgtc agccggtcca acaaagcggc cgacagcccc gccttgcggc cggcttccag
     1741 gtcgatctcg tttttgaaaa ggatctcatc ccgccgacgt tccagcgccg aagcgatcga
     1801 ttccagggcg cggttccggg ccgccgtcga ggtcagcgcc agcagacggc tggcctgttt
     1861 cgctttcagg cagacgccgg cgatctcttc acggtaatcc gcgggagtgg agagggtgac
     1921 gggccggaag ccacggacgg attttcttgg ttttttcttc ttcgccataa ggcggttatt
     1981 ttacaaaaat tcccagcccc ccggcggagg taaaaatgcg ttgcttttta tgtttcatcc
     2041 ccgctaaaaa acctgtatgc ctcaagggtt ccccacccct gacccgaaac gttgccgggg
     2101 attgcccggc agcaactgac ttttttgcgt gcccaagaaa agtcaacaaa agaaaggcac
     2161 ccctgcgggc actcactcga caatttgcgg cggccgcgaa cttaacatcc tcaaaaaccc
     2221 atcgaacgct tcggacatca cggcctgccg gcgccgaaaa tcgctgagcg aggggtggca
     2281 aggggcgtct ttctttgtcc agggcttctt tggacgagca aagaaaggga accccgatgc
     2341 ggggcgggaa gccctgcgta tccgcaggct cagaaccaca aacagaaata gcacgaacga
     2401 atttttgcta aaattattga atacgcactt aagcgcacaa aatgacgact cccatggaaa
     2461 acagtttcaa cagcgccggc atacttaccg tcaacggaaa aaaacacgcc attttccgcc
     2521 tctccgcttt ggaaaaagcc ggcctgacaa aaatccatca acttcccttc accgtccgca
     2581 tcctcctgga aaacctcctg cggcacgagg acggccggct ggttcaaaaa gccgacgtgg
     2641 aagctctggc ccgctggtcg cccaaatccc cccccacccg agaaatcccc ttcatcccgg
     2701 cgcgagtgct cctgcaggat ttcaccgggg tgccggtggt ggtggacctg gccgccatgc
     2761 gcgacgccgc caaaaagttc ggcgtggacc ccggccgcat caaccctctc ttccccgtgg
     2821 acctggtcat cgatcattcc cttcaagtgg agttcttcgg ctcgcccgac gccttccaaa
     2881 agaacgtgaa actggaatac caccgtaatc gcgagcgcta caccttttta cgctgggggc
     2941 agaaaaccct gagcaatttt cgggccgtgc cgcccggcgc cggcatcgtc caccaggtca
     3001 acctggaata cctcgcctcg gtcgtatgcg ccaaaggaga cgccgtctcc ccggacacgg
     3061 tcatcggcac cgattcccac accaccatga tcaacggact cggcgtgctg ggctggggag
     3121 tgggcggcat cgaggcggag gccgccatgc tgggccaacc actcaccctt ttaatgccgg
     3181 aagtagtggg atttaaactc acgggccgtc tgcccgacgg cgccaccgcc accgacgccg
     3241 ttctcaccgt cacggaaatg ctgcggaaaa agaacgtggt cggcaagttc gtggaatttt
     3301 tcggcgaggg actttcggcc ctttcactcc cggaccgcac caccatcgcc aacatggctc
     3361 cggaatacgg cgccaccgcg gggctcttcc ccgtggacga ggaaaccctc tccttcctcc
     3421 gcctcaccgg ccggccggac gatcatatcg ctcttgtgga agcatactgt aaagagcaag
     3481 ggctcttccg catgcccaac gaccccgcac cgctctactc cgacactttg gaactgaacc
     3541 tctccgcggt cgttccctct ctggccggac ccaaacggcc gcatgaccgc gtgccgctgg
     3601 cggaagcgaa acaagctttc aaaaaatctc tctctgcgcc ggtgaaagag cgcgggttcg
     3661 aacttccccc cgagtctctc aataaacacg tcactctgcg catgggcgaa agggcggacg
     3721 aagtcggcca cggcgtggtg gtcatcgccg ccatcaccag ttgcaccaac acttccaacc
     3781 cgtcggtgat gatcgccgcg ggactcttgg ccaagaaagc cgtggaaagg gggctttcgt
     3841 ctctccctta cgtaaagaca agtttggcgc cggggtcgca ggtggtcacc gattacctca
     3901 acgccgccgg gctcactccc tatttggaaa aactcaattt ccatctggtg ggatacggct
     3961 gcaccacttg catcggcaac agcgggccgt tgcccgaacc ggtggccgcc gccgttaagg
     4021 aagggaactt ggtggctgcc gcggtgctga gcggcaaccg gaactttgaa ggacgggtga
     4081 acccgctcac acgggccaac tacctggcct cgccgccgct ggtggtggcc tatgccctcg
     4141 ccggcacggt cgacatcgat ctctccagcg aacccatcgg cacggggaaa gacggcaaac
     4201 cggttttcct ccgcgacctc tggccgtcga acaaagagat ccgggaggcc atcaaaaaag
     4261 cggtgaaacc ggaacttttc cgccggcgct acacccgcat cgccgacggc gacgagaact
     4321 ggaactccct gcccgttctc gaaggaaatc tctatgcctg ggacgaaaaa tccacctaca
     4381 tcaaacaccc gccgtatttc cgggatttcg ccctggaccc ggccgagctg aaagacattc
     4441 aagggctgcg cgtgctggcg ctcctcgggg attccgtcac caccgaccac atctcgccgg
     4501 ccggcaacat cgccaaggac agcccggcgg ccaaatacct catatccctc ggcgtggaac
     4561 ccaaggattt taattcctac ggcgcccggc ggggcaacca tgaagtgatg gcccgaggga
     4621 ccttcgccaa cgtgcgactc aagaacctcc tggttcccgg caccgagggc ggcgtcacgg
     4681 tccatcggcc cagcggggac accgtctcca tcttcgaggc ctcccagcgc taccttaaag
     4741 aaggcgtgcc gttgctggtc atcgccggca aagaatacgg gtccggcagt tcccgcgact
     4801 gggccgccaa gggacccatg ctcctgggcg tccgtgccgt catcgccgaa agctacgagc
     4861 gcatccaccg cagcaacctg gtcggcatgg gccttctgcc gctgcagttc attccgaacg
     4921 aatcccgcga gagccttcgg ctcacgggac gagagacctt cagcattacc ggcatcgccg
     4981 aagggctgac tcctaaaaaa gtcctctccg tcaccgcgcg gcgcgaagac ggctccacgt
     5041 tcacttttaa cgccgtggcc cggctcgaca cccccgtgga ggtggactac taccgtcacg
     5101 gcggcatcct ccaggccgtg ctccgccccc tctgccgccc gtaaaaaaac ttccctctcc
     5161 cctcgaaaca aggcccagta aaacaatcgg cgttatctcg ccgacgacga actcattttt
     5221 attgaggaat tacgattttg ggggaagggc gggggtggag gaagcgggga ggtcgggggt
     5281 tgagtggccg atggaagcga gtttttcttg gaagcgctcg agacgttttt ccgcgtcgtc
     5341 gtatttgcct ttgacttttg acacatggcc gcccaacacg tcgaaatctt cccgaaaccg
     5401 gtggaaatcg ccctgcaagc gggccaggtt ctccatgatg accttggcgt tctcctcgat
     5461 ctcaaaaccc cttaacccca gaatgatggc ctgcaggtag gcgtagaacg agttgggcga
     5521 caccgggatg acccgccgtt tgagggcgta ttccgccagc gatttcccct cttccgagtc
     5581 ctcgtccttg acgatgatct cataatacac gttctccgcc gggacgtaca tcaacgcgaa
     5641 atcgtaggta tcctcgtccg gcaggatgta tttgtcggcg atgtcgtcga tgtgcttttt
     5701 cacgtcgccg gcgagttttt tcttgagagg ttttcgttcc tcgtcggtct tggcgtccat
     5761 gactttgcgg aaattctcca gaggaaactt ggcgtccacg gggacgagtt tgccgcccag
     5821 ccggacgacc gcgtccacca ccccgccgtt cttgaaggcg tgctgcagtt cgtaatggcg
     5881 cgtgggcaat atctgagaaa gcaggtcgcc caggaagaat tcacccagac cgccgcgcat
     5941 cttgggcgcg cggaggattt cctgcagacc ggcgatgtcc ttgcccacgt cgaagatccg
     6001 ttccgcggcc tgccccagcg tgccgaggga tttctgcacc tccgacacca cccgggcggc
     6061 gttgtcgagg cgttcgccca cgcttttttg tgaggtcatc atttggctgg agatattctc
     6121 caggcgctgg ctcacttggg tggtcacctg gtggagttgt tggttgacgt tctgcgtctg
     6181 ccccgaaagg gtctcggcga gctgggatcg gagtccctcc aactgctgct gcatgagctg
     6241 gaggccgggg tcggcggcgc gggtgttctg cgaggcgcgc atcatctgcc ataccagaaa
     6301 aatggccgcc ccgacgaccg tcaggccgac gaacaatccc acaaaagcga gaatctggag
     6361 gggggtcatt tcaggcggag atcaggcgtt catctgccgg aggaagaaaa tccacgccat
     6421 caaatcgttc cggaggccga gggtctcccg aaagaggagg tgatactcct ccgccgacat
     6481 aaaccggcgc ggaaggcgcg agacgaaccc gacgcaggcg tccgcgtggg tcagcgcccg
     6541 gcggcagcgg gcgatgttgc ctttcacggc ggcggccccg taacccacgt aatgcccttc
     6601 cgccacgttg tgcgggatcc gccgggcatg gtgccggatg tcttcgagga gcgcctggca
     6661 gagccgatgg agacggctct tataggtttt ggccatcatt ttttgaaaac gccgttccca
     6721 cagggacgcc accacgccca caaaggactt cgcccggatg tagatgggga gggcgtccac
     6781 ctcggcctct tccagagaaa gacggctctt ccacaagggc ggttccgtgg acgggccagc
     6841 gggcggctcg aaactcgcct ccggcgcgaa aacgccgtcc gggtccatgc cgaaccggcg
     6901 catgacgttc cggaacgaga gggacgggtc cacggtccgg ccgcgggcat acgcccttcc
     6961 cgggcggacc tgtcggacga actcgtcctg ggcggagaag aatcgttccc aatcctcttc
     7021 gtcccatttc ttgttgaagt tgatggactc gaagcagttc ctgtttgatt ccatagcgtt
     7081 ttccgtcccc gccgcgtccg gcggagcgtt ttagaacagc gtgtagatga acggcgccac
     7141 ggccgacccc tgcgccaaaa acaacaggac gccgaacagc accagcacgc tgaccatggg
     7201 gatcatccac cagagtttct gttcccacaa atagtcgagc agttcccgaa aaattcccaa
     7261 tttttctttc agacccattt ttgcgacctc ccgagataag cctactcgcc gatgatcttg
     7321 accacgaccc gctttttccg ctggccgtcg aattcggcgt aaaaaatctg ttcccacggc
     7381 cccagatcca gccggccgga ggtgatgggg agcaccacct ggtgatgcaa gagcgtgcgt
     7441 ttgaggtgcg cgtcgccgtt gtcctcgccg gtgcggtggt ggcggtaatc ggccggcggg
     7501 gccagccgtt ccagccagtc gaacacgtcc tgcttgaggc cgtcctcctc gtcgttgacc
     7561 cagatggcgg cggtgatgtg catggcggag acgaggcaga gcccttcctt cacgccgctg
     7621 gccttcacat attttgccac atccgccgta atattgacca gttctttccg cgaacgggta
     7681 ttgaaccaca gatattcggt gtgggtcttc attgtttttc cttctccttg aagacgcggg
     7741 cggaacggcc gagcttgtcc tcgccggcct cgtggccgcc ggagagatta taggaaagga
     7801 gttccgtgtt ccgccgctcg ccgccggagg tggccagcgt ggtgaggacg tgtcccacgc
     7861 cgggcgcgta atagttcagc tgccaggacc ccgtgatgcc ctccatctgt tcacgcacca
     7921 ccagacagtc cctgaactcg ccggccctca ccttcacggc ggccatcccg tcgacggtaa
     7981 aaatgaaatt ccggccgtcg cggcgcgacg tccagcggct gcccttttcg aacggatatt
     8041 tcaaaatgac cgtgggggcg cttttgtcgt tcagatactc gcgcagttcg agtttttcct
     8101 tcttgtatcg cttgcggatg gaaaggtttt tctgaaactt gtcgccggca taaaagttgt
     8161 gcgtcatcag cacggccgcc gggtcgtcgt cggccacgcg gcagacgatg cgtattttgg
     8221 cgttgcggcc tccggtgtcc gaatccccct cctcccactg gccgttgacc accaaaggga
     8281 aataattagg ggtgttgaag atggcccgtt cggccttgtc gccccagcct ttccggtccc
     8341 cgtgctggtc gatgagccgc tggaaaaggc cccgcgccag ttcgtagtcc tgcaacgtgc
     8401 ggcccgccaa ccggccggcg gcatagagcg ccttgggcgc gatggccccg gacgggactt
     8461 ggtccgccac ggccacatag gcccgcagcg cctcctcaaa acgtccggcc ttttccaacc
     8521 gttccgcctt aaaaaagccg agacggccgc aacccaacac ccccaaaaag gacagcgcca
     8581 gggcgacccg gcccgggcgg gtgtagaacg gggacaactt cgcgaaaaga ttcatccccc
     8641 accgccgctg ccagcggcag accagaaggg cgccgagcgc atcgataccg gcgtcccacg
     8701 cggagggcac ccgtccgggg atgaagccct gatgccactc gtcgcttgcg gcatagagga
     8761 tggaaaaact cacgcagacc caaaaaatcc ttgaaagcgt ccaggaaaag gtgtgttcca
     8821 gcgcgcgcca caacaaagcc gccagaatac cgaactccgc cgcgtgagcg gccttgcgga
     8881 aaaccaaatc ccacatcccc cagcccgtcg acaggtccgg gatgccggag aaatagaaaa
     8941 tcactccgca ccacagaagg ggcggaatcc agagacccag ccaccggttc gccttgtttt
     9001 tcgatatcgc cggttcgctc acaaatcgcc tttttcccgg aagctgtggt gtttctgggc
     9061 ggacacgatg acgtggtcca ggacttcgat ccccaaaagt tcccccgcct gcgcgaggcg
     9121 tttcgtcaac cgccggtctt cttccgacgg cgtgggatcg ccggaagggt ggttgtgcgc
     9181 cagaatcacg gacgcggccg aatgccggag ggccggttca aaaacctccc gggggtggac
     9241 caggctggcg ttcaacgtgc cgatggagac gaactcctta tgcgccaatc ggttccgcgt
     9301 gtcgagatac aggacgacga aattctcttt ttttcggtcg cggatttcgg cgaggtgctg
     9361 gcacacgtct ttgggggtct gcaccgaggc ggcctcatcc ccggaaccgg aatggcggcg
     9421 ggccagttcc caagcggcca gcaacgtcgc cgcccgggtg tcaccgacac ctttgatttt
     9481 ggccagttct tcaaacgaca actcgaagag ttgcttgcca gggtgctttc gcaccagctc
     9541 gcgcgaaaga gtcaagacat ctttcccctt atagcccgtg cgcagcagga tggccaacag
     9601 ttcctcgtcc ttaagcgcac ccggcccgcc ccgggccagt ttctcgcgag gcttctcaat
     9661 acggtgaagt ctattgaagg atgccgaggt cagcactttt ttctagaggg cgggaaaccg
     9721 ataaatctcc gcgcgaaaca accaagttca actccgcttc cggagacaaa ccgccgtttg
     9781 atcgtcgttc atgcgcgcct gttcccagag gttccttcgg gctacaattt tccgccgcaa
     9841 ttcccgcaga aacgcgttcc ggcagggctc tgagccccgc acttcgaaca tttcaacatg
     9901 ctcagatcgg cgccgcagtt gttgcagaat ttcccctggc ccgccggttt cccgcattga
     9961 ggacacaccg tttgtttgct ttcgatctcg cctgaaaaaa cctgtttcgt atcggcggca
    10021 ctctggatgt ctttcaccat ttttttcgct ttggcggccg ccacttcaac gttcattcgc
    10081 ggcgcatcgt cgacacagag cccctcctgt tcgttccagt ccgtttcgca aacccatttt
    10141 ttgcacttcg ggcatcgttt gaaatgtcct ttggcctcgt tctgcgccaa ttcaaatgct
    10201 ttttcgtgtt ctttgtgcca ttccggcgac atcccacgat acttttccct caaggcgtcc
    10261 gctccgcgct ccaggccata gccgacaccg cttttgccga gcatattggc gatcgtaaaa
    10321 gccgcctgcc ccaggccgcg caggagcccg gccgttttat ggctcttcga ttcggtgaat
    10381 tttgtcttat atccctcctt gcacgaatcg cagaagaaag tgaattgaaa ccccgcttcc
    10441 gtgctgttgt cggcgaaatt accggtgaat gcctgcagtt ccattgaagt ctcctttatc
    10501 tccgctttga tccgttattg aacctgtttc aatgactttc cgcacttgat gcaattaccg
    10561 tccagcggcg gctgttctgc tttgcacttg ggattggggc aaataaccgc caaccgcgcg
    10621 tcgcacaacg cgcaatagtc gccgaagaaa gtggccttgc cgcatttggg acacgcaacc
    10681 tccagcttca acccacaatt cgcgcaattc ttatattcct tttccacagg catgcgacac
    10741 ttcggacatc tcaaggcgtc caaaggattg atcttcccac aaagaggaca tactttcgac
    10801 tccgggggga tcaatttgcc gcaatatctg catggatgct tgtaccctgt cacgatcgaa
    10861 ctcctcttgc ttcagaattg acggtattgt gaaacatcga acaaaatcac gccttgtctt
    10921 tttggcacca ccgggaaccg tcttcggctt cgctgttaaa tttcaatagt tcggccacat
    10981 cctctttcca ttggccatcg gaggagtgga tgacgcggcc gacatagagc tcacgcagca
    11041 tcctcaggcc ggggacgccg gcgatggcat attcgtattt ctggtattgc ggctccgcca
    11101 gcttttccgg cgggagatag atcggttctc gacgaaaatt aatggccaac atgcaccgat
    11161 tgatgttccg tatcgcgttg tcggcttctt tatggagctc ttccagcttg agagactttc
    11221 ggtagtcttt ccggctcacg atgcggtaga agtaagtcgc cttcccggtt tccttctcgg
    11281 tgaccgactc catggcgata aggttgccgg ggacgcgcgg gtcgtctgaa tatatcggag
    11341 cgaggaacca aagatactcg cccgaagaat cccgctttag tccgatgccc atccgggtgt
    11401 cttgtgcctt gcttctgaga aattcatatt cctctttcaa ttccgtaagc gccattttcc
    11461 tttccaactg ccgccaaatg tccggggaaa tggcgtccaa atccgacttt ttcgccgcct
    11521 tgccttctcg catcaaaccc gccgctttac gcaatatttt cgggtccgcc cccggaacga
    11581 tctccctcag cgttgtttgc accatcaagg caagctcccc catccggtcg gacagcgcct
    11641 tggcgaaggg gccgaactcg tatcccatgc cggacaaaac atatttttcg ccgagttcgg
    11701 tcttgatctc caacttgaaa tcgccctcgt tcgtctcgag aatatcgcag aaagggatcc
    11761 gaacggggtc tctgtctccg aacaataaga ccaacgctgt tgagcaaatc cgcaacgcga
    11821 cgttcttggc ttcgataact tgcccgtcgg cgccgttccc cgacaaatcc gctttaacgt
    11881 cctttttaat cgtcgactct ttcatgagga ggtcgcgcag cagcatttcg ttgcgaaaac
    11941 cggtaaatat cctgatgagg tcgtcgtatc cataaccgat atccgtcagt ttcagtcttt
    12001 ctgcggagga caaggcgagg gatatggcgt ggtcttttgc ggaatattcc aaaacatcat
    12061 ggtaggaaag gcagagcggt tctccccgcc ggggacgaac gaacatgcca accggagtca
    12121 gcttgagcgt ggcgtctccc gaaaccgccc cttctccggg tccctcttcc agactgtatt
    12181 tcagcggcgc ttcaaacagg atttcttctt tatcttcggg cattgacggc cgaactcctt
    12241 taagggacta ttttacaaaa atgtactcta tcacccgccg cacgccttcg gcctccacga
    12301 cgatcccccc cgttccttcc cttagagcct tgacttggac ggcgtccccc accgatggcg
    12361 caatctcaag gacttcgggt ttgacggaag accatttcgg aacgatggcc aacggtttat
    12421 tgttcatgtc gcggccctgc gcggttagaa atatcctgcc gccgatgttc atgtttgaac
    12481 cgtccggagt cgggccggtc tctcccagcg gcacattccc gacaaacacc aaaatctttg
    12541 ccaacggtcc ctttgcgagg ggattttcca actgggggat ccgcgacctc aaactgctgc
    12601 aggcgaccaa aaccgccaat ccggcaaaaa ccgcgaatat ttttgttcga tttcccattt
    12661 tcattctcct ttgaagtttc cgagacgacc atccgctccg tctcaacaat ggcccaccat
    12721 caaaaaggca gcgcccgttt tccgcccttg ctcgctttga cgatcttgtc gaattgtact
    12781 catttcgccg tttttatccc agcataaata cggcgaacgc cgcgcgaggc aggcggaacg
    12841 gcgatcccgc tttccgcagc agcacccccc gctttgaacc aacgcccccc ggaatctttc
    12901 cgcacgacgg cgcttacgca tcgatccttt cgcccccatt tagttcaaca tagcgccacg
    12961 gccgacagag tcgcggtccc caaacacccc cctgagaatc gcatccgcga gattctccgc
    13021 gatcagtctg ttccccaaat ccgtgcaatg cccgaaattg ccgaaaaaca agtccctgaa
    13081 tatctccttg acttcatgtg tctcgagagc cttttcgaag ttggccacgt tttccacata
    13141 ccggatcccg gcctttttcc caaaaacttt ttccagatcc gccaccgggc acctgggata
    13201 ttgcatgcaa accagtttaa ttcctctgcc caaaacgacg tctctcaact ttctatatcc
    13261 gcgcagacta ttcggaggaa gacgatagtt cgcgccccca tacttcttcg ccagttccgt
    13321 ttttccctga agaaaatata tcatggacaa tccctgttcg aattcaggcg ttaacaaata
    13381 tccccggttg aacacctcct ccgccttctc atattcctga cactccaaat aaagcgtccc
    13441 caggctatcg taggcgtagg gattttccgg aaaagttttg atcatctttt catagatttt
    13501 ttttgcctct tcgatttccc cattcatacg gtgcagttcc gccaattcaa tatagccccc
    13561 cagcaggtct cggggagctt ctttcatgcc ggcaacgtac atcctcttcg ccctatcgta
    13621 ttccccccga tcccgatata aattccccaa ttgaaagtaa gcctnnnnnn nnnnattaac
    13681 agcccttatc cccgccctgt atagctcctc ggccttctca tcatcgtcct tggcctgata
    13741 aagtctccca agcaacacat atccgctcgc atccccgggc gaagcggcta tcccttgctt
    13801 aaacatcttt tccgccttat caaatcgacc ctccccccga tatcgtttgc cccaacgtat
    13861 atagtcgtcc gcctttaaag atgcggcctc ctttggggga gcctcctctt tcggccgcct
    13921 ttcgagccat tcgaacacct ttataagctt cctttctcca tttctccgtt ccccctcccc
    13981 ccatcttccc accagcaatc tcaccccggc ccaaaacgaa tcaaacctgt cggccagcca
    14041 gcccccccat cccgtttcct tcgttcccca gcgtccctcc agcatcccca tcatcaccac
    14101 taccatatcc ggctgatact tatccagatc tccctccaga aaatacagaa cccgcgagat
    14161 acggttaaaa actcgccccc tgtcgatcac cttgaaccga atattccctc cgcgccgatt
    14221 caactcttcc tcgaggatcg gcggccattg cgcctgggtc gtcgaatctc caaaacacaa
    14281 tattttatat ccgccttcgg cggaaagtcc tttaagattt cgccgttcct ggagaaacag
    14341 actggaacga ttgtatatcc ccacgcaagc aaacagaatt atgatcgcaa gccccgcttt
    14401 caaagcggca ttccccaaga attttatata cgctcgaaaa tcatcgttca agaagctggc
    14461 cctgcctttt caaaattgaa agtaaataaa cggtgcatga ttgaaattcc cgaaaacgaa
    14521 aagcagcgtc aagaacacca aatagatcag aaagcgcccc caggcgggga cacgttcgat
    14581 cgccacggcg tcctccctct tccgctgtac ccattcgaca aacacgacca gccccatcaa
    14641 aaaaaacgct cgcccgcaga acgcatggtc cggcaggaga actgacgcgc cccctaaaga
    14701 acgaaatacg ttccgatagg cggcaagagc gtccgacaat gtggccgccc gaaaaaaaac
    14761 aagcccgcag cagaaaagat ggaatgtcgc cgtcatgttc aaaaagcgag ccgcggcgtt
    14821 ttcctgccgg aaaggtttct tcatgaaata aaacacgccg ttaacaagcc caaagacgat
    14881 aaacgtccac tgtgccccgt gccacaggcc gcttgcgacg aaagtgatta aaacattccc
    14941 cgcccacctc gaaaccggtt ttatccgccc ccccaacgcg aaaatataga catagtcccg
    15001 cagccagttc atcaatgaaa tgttccagcg cttccaaaag tccctcacgg actcggaaaa
    15061 aaacggactc cgaaaattct gcgtgagccg caccccgaag aacaaggacg ctcccatagc
    15121 tatataggaa tatccggcga aatcgccgta aatctgaaaa gcgaaacagt acacgccaaa
    15181 aacgatttgc ccgccggaac agcgggcaag gtcggcaaaa accccatcga cgacgacggc
    15241 cagattatcg gccacgacgc atttgagaac gtatccccaa agcatgcagc gaaatccttt
    15301 ttgaaccata tccatgtcaa aatgccgttc ccgcatgaat tgcggcatca gatttcccgc
    15361 cctctcgatg ggcccggcca ccagttgcgg aaaataagcc acatataaaa agtagggaag
    15421 cggctctttg gtcgccggaa tggcataccg gtaaacgtcc aacgcgtagg ccatcgtttg
    15481 aaacgtgtaa aaactgattc cgaccggcag gatgatgttc aatgccggca tgtgcggaga
    15541 aaaacccagc acacgcagga aagcggcaga agactcgaca aaaaaattga agtatttgaa
    15601 aaacagaagt aggcccaggt tcacggacaa actaacggaa agtatgagtt ttcgcgtccg
    15661 cggcctttgc catccttcct tagccaaacc cagtccgcat agataatcga ccaatgacga
    15721 ggcgaacagc agaagcaaaa aacgataatc ccaccatcca taaaatatgt agctggcaac
    15781 gagcagaagg agattacgat acgtattttt tcggatggcc cagtggaggg tcagcgtgat
    15841 gaaaaaaaag aggagaaaaa ccgtggaatt aaatagcata ttctctcgac ggcggaattc
    15901 ccgaaacaat cgttgccgca aactttattt tttcagccat tttgccatca tcaaacgcgt
    15961 tctccgccta atttaaaacc cctatttccc cgaggtccac gccgcagcat ttctcagaca
    16021 gctcatcaat aaacccgggt tttccatcat atgacgtctc gatcaatttc tctttcccgg
    16081 tttcgaggtc ggcatccgaa acccataaac gattctcaga cccggcaccc acagttcttt
    16141 ttcccgagcc gagcccgctg ctcgcgcaaa gacgccagga gaaccttttg aacggcggct
    16201 ccggccgcca attccctccg cagcggcgat cgatgctcgt atccccgccg tcccatttcc
    16261 tcgacgagtt cgtcatgccg cccgtagagg gcacggagct tgcctttcca gcgaagcgtc
    16321 tccgggtgcc ggctgtatcc gctttttttc cgcgtaatga tgttccacac ggcatgcagt
    16381 tcccggtgct ccccaagcag atgcgcccga cacagccgcc aaggcggaag atcccatatc
    16441 ctcatataaa aaagttcctc caaaaatatc cgcggtcccg ggcatggccg gtgaacggca
    16501 accttatggc ccagaatccc tgatccattc tatgaaactt tcgattcgcg cgtaacgcga
    16561 accgggaaaa accgattgac gacccgggtc aaaaactgtt tactgttcaa aacgccgaca
    16621 gtttttcgtg cgccaaaaac gacggacgag aggctcctcc cgtgaaaatt gttttgatcg
    16681 accccccgtt tgaagggccg gattctctgg gcaacaacgt cagtttggcc ccggtggtga
    16741 acgtcgttcc atccatgggg ctggcctatc tggcggcggt tctggaaaaa gccgggcatg
    16801 ccgtaaaaat actggacgcc actctcggtc tttcttccgc ttccgttctg gacgaaaccg
    16861 ttcgatttgg acctcaaatc atcggcgtca ccgccaccac ccccaacttc cgcagcgccc
    16921 gccgtttggc ccaggaaatc cgccggcaac ttcccgaaat cctccttgtg ttgggaggcg
    16981 cccacgtgac ctgcgccccg gaacacgcca tgtctttcgg ccttttcaac gtcggggtgg
    17041 tcggcgaggg cgaagaaacg ctgctcgaac tagtgaaaac atacgcggcc cgcggggcag
    17101 cgggacttga atccgtcccc ggtctcgtct tccggcgcga caacgacctg atacggacgc
    17161 ctccgcgacc gttcatccac cggctggacg agctgccgtt cccggcgcgc catttacagc
    17221 cgccgctggc cgcgtaccgt cccacgccct gcacgtatcg gcggctaccg ctgggcgtga
    17281 tgatcactag ccgcggatgt ccgagcaaat gcacgttctg cgaccgctcg atcttcggga
    17341 cgaaataccg gacccgatcg ccggaaaacg tggttgccga attaagcgag gtcgtccgca
    17401 aacacggcgc tcgggaaata cgattctacg acgacgcgtt cacgctggac caccgccggg
    17461 tctacacgat cttggatctc atggccaagg aaaaactccg gccctcctgg acctgtctga
    17521 caaaagcgag ggggatcagc cgggacttgt tggcggccat gaaagcgggt ggctgctggc
    17581 aggtgcaatt cggcctggag agcggcgacc ccgagatgct gctccgtatg ggaaaaggca
    17641 acaccgtgga ggacaatcga cgggccgtct cgtgggcgcg aacgctgggc ctgacggtgc
    17701 gcggagactt tgtggtgggg actcccggag agaccgcgca gagcctggaa cgcaccttct
    17761 gccacgccgt caaattgaaa ctggattatg cctacttcca caaattcgtc ccgtacccgg
    17821 gctctgaact ttacgaaacc ctgaccgccc gggggcaccg gttcgatttc gacaccctgg
    17881 gctcaagcgt ggtggacaac gattccatcc actatgtgcc cgacgggttg gacgcggccc
    17941 attaccgacg ctggctgaac gccgtgtata aacggttcta tttccggttc gggtatgtct
    18001 tacgccgggc gatggcggtg cgaagttggg aagagtggcg cgggcaggtg cggggattcc
    18061 aggcggtggc gggcctaaag acttaatcga ttttttcttg ggacgtcctt tcccttccgg
    18121 ggaacttcct cgcctaaaaa ttcatctccg gatattccgg ccgacccaga ctagtcatcg
    18181 tcgaaaaacg tccgaacata ccccctactg gcccgcaccc caagagatcc tttcctattt
    18241 gggggcgggc gggggagccg aagacttttt cagttctcgg cgagcgcggg ccagcaagga
    18301 tttcgacggc gggtgattgg gctcaagctt caggacgttt tccagatagc ggatggattc
    18361 tttgtagttc ttctgcatga agtagtcctg ccccttgatg taattctcct gcatgaggcg
    18421 cagtttttct tccgaaggca tggcctgggc cgccgccggc ggggccggga ccggaacgga
    18481 gaccgcggcc ggcgcgggag cgggcggcgg ggtcggcgac gcctcttttt tcgccgccac
    18541 cggcgccggc ttgtttttct caacgagcgc cgcgatggcc tgatagatgg gttcgttggc
    18601 gggcggtttc aggcgcggag ccgtctgcat cagcgtgggg acggcggctt tgtttcccac
    18661 ccggccgatg gcggtggcca aggcgcagat gacgttgggg tccatttccc tctgaatggc
    18721 ctcggtgagg ggcgacaagg attccggaac cgccaggatc cccatgacca cggcggccga
    18781 ctcacggacg gccacctctt tatcgttgag cgccaccgcc aaagacaggg aggaaggcgc
    18841 acccccgagg agccccaaag aaccgacggc cgccaggcgc acggagacat ccgggtcctt
    18901 cagcgcccgg gaaagatccg tgatgacggt ctcgtcgcca atctggccaa ggatgttcac
    18961 cgccgacagc cgcaggccga ggactttttc gttggtggcc acgcggagaa gtcccggaaa
    19021 ggtttgactg ccgccgatct tccacaaatg ggagaccgcc aggacacgga tgtccttatc
    19081 cggatcatcg acggctttgg tcagggccgg gatacaagcg gagtcggcga gggtccccag
    19141 ggattccacc gccgtgcggc gaacgtcggt ctgccggtcg gaaaccgcct tggagagaac
    19201 ggggacggcg tcttttgatt tcaggtcggc gaggactttc accgactccc gccggacggc
    19261 gtcgtccttg tcgccgagcg cgggctccag ccgaccgatg acgtccgctt ttgatttttt
    19321 ggcgatgttc tgcacggcca gaacgcggat gttcgcctcc ggatcgttga gcgccttcga
    19381 taggccctct gacgccaacg gcagggagat gtcgcccagg gccgccacgg acgcctcgcg
    19441 aatcttgtcc ttcttgtctt ccagcaaacc gatgagcacc tccaccgcct catcgttccc
    19501 gacttccctg agcatggaga caccgtcgag ccggtcgttc attttcttgc tgttcaacaa
    19561 agaccgggcg gtccccgcat cggcggcgga caacggggtc ctcattacga aaacaaaaac
    19621 ggagagaaga actgacgcga tgaggccggt tttcacct
//
LOCUS       DAOTFD010000023        19623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1819_length_19623_cov_4.507052, whole genome shotgun sequence.
ACCESSION   DAOTFD010000023 DAOTFD010000000
VERSION     DAOTFD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 19623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19623
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_19623_cov_4.507052"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="P5079_02700"
     CDS             complement(<1..435)
                     /locus_tag="P5079_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY28928.1"
                     /translation="MQTVNQFMGADHDRLDDIFRRFQDVRKKDLSGAMDLFRAFRSGL
                     LRHIRWEEDLLFPSFEKETGMALSGPTQVMRMEHREIKLDLEKIGADLLNGNSGQWET
                     PLLDVLSDHNNKEEAVLYPWIDRSLTAEEVESLVLLMKADVPK"
     gene            647..2071
                     /locus_tag="P5079_02705"
     CDS             647..2071
                     /locus_tag="P5079_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRY28929.1"
                     /translation="MTPQRASKSSPGAPLLALAMAFSLLSGAARAADLKKGDPCPSFQ
                     LVPLASTASAGIPLEKLLENGEPVLLSFFAAGCVPCLTEIPTLEYIAQTQNVDAYLIS
                     VDSAPADRVKSLALKHNFTLPVLLDPAGKRAGLPYGAVPGPQTTSPQLFLLSPDKTLW
                     WRSQDSAAGLEKELTNQIRDLNEALARRVPAAPISLDSDSFLLVFTNSANGYLSPCGC
                     ADLPFGGVSRRSTFFKGLRKKFPDPSLLFLDAGDNYALRAPSLQSVMDVLDVLRYDAM
                     VLGDQDILLSGKDARKTLFNRSRPFLAANFEVCDDAGCDAPLPHKIFKRGDLRVAVIA
                     AMDPDIYTLAPKTWKKWVHLTDLRKKLQESLPSLRENNDVVILLSHSGLETDCSLAEN
                     IPGIDIVVGGHSQTALKDAIRVTNLNSGGHAFVVQAGPNGQYVGKVVVRMQPDKTVEL
                     TGYELVPLTDAIEDDAEVLKLLPQ"
     gene            complement(2154..4391)
                     /locus_tag="P5079_02710"
     CDS             complement(2154..4391)
                     /locus_tag="P5079_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY28930.1"
                     /translation="MKRKIVVVGGVAGGASAAAKARRVNEEAEIVMFERGPYVSFANC
                     GLPYYIGGDIQERRSLLLQTPESFWKRYRVDARVGHEVLRLDRAAKTVEVKNLKTGDV
                     FREAYDALVLAPGAGAVRPALPGIDAKNIFTVKTVPDSDAVKEFLRSHRPARAVVAGA
                     GFIGLESAEALKRLGLDVTVVERMPQVLPPFDPDVADSVARHLEEAGLTLVLGDALKA
                     FHGDPLATEIELASGKRIPMDMAILSIGVRPELKLAEEAGLAIGPSGGIVVDERQRTS
                     DPDIYAAGDAVEVVQLVTGRKIRMPLAGPANKQGRVAGANAAGGRMTFPGALGTAIVE
                     SLGISAAKTGLSEQEARAAGLRVGVSLTHSLDHAGYYPGAEPLHMKLVFEEGSGRLLG
                     AQIVGEKGVDKRADVLATALSARMTAADLENLDLAYAPQFSSAKDPVIMGGFVAANAV
                     RGEIKTVTCGQLLEKQRRGEPVQVVDVRTPAEYDGGHLESARLIPIDVLRERISELDP
                     KKETVVYCRVGFRGYLAARILQQNGFGDVFNLTGGILACPSFGRRKSAGTGAGPTTEI
                     LPDGRLTAVGLKKRLAGGAVVVDVREEDEYRYEHIGGTRNVPLGRLAAELPSFPKDRE
                     MAVLCQTGVRTAQALKILKNAGFDRVRAVQGGLEAWKDAGLAVERRRGPIPLLRQVQI
                     VAGTLALFGGLIVPLRWVAVFVGAGLVFAGISGKCGLAELLAKLPWNRHPEEEKPAGG
                     CGPCA"
     gene            complement(4469..4924)
                     /locus_tag="P5079_02715"
     CDS             complement(4469..4924)
                     /locus_tag="P5079_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY28931.1"
                     /translation="MPKATDQLLKDHKMIRKTLEGFTLGNPRFALILETLQRIILAHA
                     WFEDELFLPALEAEPLLDKRYAREIVQEHEDLAHFLRRMRRTPAAQKKEWEIDMLQFR
                     SLLDMHLLKEEAGLFPAAERLLGEERLDRLAAEMERRKAEARGAADRQG"
     gene            4993..6384
                     /locus_tag="P5079_02720"
     CDS             4993..6384
                     /locus_tag="P5079_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY28932.1"
                     /translation="MNYDAIVIGGGITGAGVLRDLALRGLKALLVEKDLPGRATTGAS
                     SHLIHGGVRYLLYDRPTTETTAWDSGHIVRIARPLLTRLPIVWPVYADHARGRTTIET
                     LLESYDRFQPLKGGLPHLRLNAEETARLVPQLKRPGLRGGLVFDEWWVDPVRLVEKNL
                     DNARANGAEVREKTAVTGLVLAGGAVKGVEVLTPRGERETLRADLVINAAGPWVDKVA
                     RLAGLSIPLRLQQGTHLVYDGNEAILRSPAGRFGLLLEAEDRERYVFVIPGGDMTLVG
                     PTDITSPDDPDHLSPQPAETAQLLGTLRRYFDSFPERCSKTIVAARPILGQTGNAKLL
                     SREFQVLDHAARGERSGFLTIAGGKMSDFRLMGEETGNAAVRLLKKGGSGRTHSIALD
                     GSSVSTEGSADRPTHPLTPFLRRHPRLRELHALAHLGVGLAGHLARRLGGKTRDDTVD
                     DFTRRYNAPASGL"
     gene            complement(6373..6768)
                     /locus_tag="P5079_02725"
     CDS             complement(6373..6768)
                     /locus_tag="P5079_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRY28933.1"
                     /translation="MKTVLLLLGSNIFMTVAWYGHLKYKAYPLIAVILVSWLIAFPEY
                     CLQVPANRIGHGTFTAPQLKIIQEVISVAVFIFFCLFYLREPMRWNYVVAFVLIVAAV
                     FFSLWEMPRVFAHGPESPEKPASSEVLEP"
     gene            6952..7350
                     /locus_tag="P5079_02730"
     CDS             6952..7350
                     /locus_tag="P5079_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28934.1"
                     /translation="MPEFSPTARQNPSILVVEDLESTREFVSFILRKQGYHVLLAKDG
                     KTGLSLSVDKQPDLLLVDILLPGMDGYSLCSRIKQDPALKRQKIILFSALNAQANKLK
                     AEESGADAFLEKPVEPARLLSVIKSLLSPS"
     gene            7440..7913
                     /locus_tag="P5079_02735"
     CDS             7440..7913
                     /locus_tag="P5079_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28935.1"
                     /translation="MRATVSSAFVIRNIAFFPAAFLLIGGLFVFQAPLAALERPALVW
                     NVAADRLETSPRQPVKASGRVVLEIKDSRVEADNVALLPNLKRAQAWKTVSLQNESFL
                     VRTEWMALDCSQSGAPCALYIDPLGNLPGERRTRTPKFWSFSGEPARAGGEETWQ"
     gene            7910..9136
                     /locus_tag="P5079_02740"
     CDS             7910..9136
                     /locus_tag="P5079_02740"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY28936.1"
                     /translation="MSPLKTPLSSRFKFGFWLGGISLAAAMCLLATSRFSESGSLAGE
                     TARLMVTSGSFSRGVTLYDALKQASLSPLLIQQIGDSLQRSINPRSFKNTDTYELVQT
                     ADAAFHHLTITRGLKQYIVSLDPSQRLALYLQEIPVHTQEEISAGSLKGSLWESMSSR
                     GMPPELIMQFADIFAWNIDFLTEPRDGDRFALVWEKGTTPDGEVTQQKILAALYEGRA
                     LGRHLAFLHNGEYYDTDGNSIRKAFLHAPLNFRRISSGFSRRRFHPVLRYFRPHLGID
                     YAAPRGTPVVSVGNGTVVFRGWKRAYGNFVQIRHNGAYTTCYGHLSRFARNLRRGARV
                     SQGQVIGYVGSTGWSTGPHLDFRVLKNGRFVNFIKLKFPSDKRIPASENEAFQELGRQ
                     RREEMEDFLKEHLAAE"
     gene            complement(9155..11695)
                     /locus_tag="P5079_02745"
     CDS             complement(9155..11695)
                     /locus_tag="P5079_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28937.1"
                     /translation="MSSAVLSGLRAEETGVSGSTGASPASASWKKRPVRLPPLFWYEA
                     DPEEERRLLMLSMLYWNVQDRSSSYKLMLPVFYRWRESDRSLLVSLPLVFSYRRPTEK
                     WMVAGLFFRHSDPRVTHTALFPFYWQKIRAEGGRVTTLPPFLFYDYRSGDRQKVDQVS
                     LWGWSRRRGDRRLGFLLNYAWFDDPGVRTRVFFPAYWRFRNPGGKLDVLAPFYWKRSA
                     PTAPPQTDMGLFPLAGAGWGARRSHYLLPVYLYSRGEKSRSFLTLPFSSFVNDDQRAG
                     HLGLYFYSRDPDLRLSGVFPLWYLRRSADGFEEKTQILNFYQRRENEDYFQTLFPLYG
                     YWSDPDGAHLVSWLALWRRKEGSSSGWSYLYHWRNADNGDRTRVFFPLYWHFRRPPDW
                     GVDVLFPFYARTRDGDSTLTAVPPFLWKKTRDRRTWSLLFLYWRDHQADRGSTTLFPL
                     FHYNYNARRRLFFSPLAWTRRSALSREGIVLPAYWYRSAESKRFIFFPVYWGSATPSK
                     SLTIVPPYYRWRREERRAYGLFPLWGRDFSPEERGGYLFPFYWYSADKKGDGLWIVPP
                     LLVYLSRGGTGTDRPRSHLQYLLLGGVAREGENLEHEFFPLYKYIRRADFRNFWAPRG
                     IALAAWERQGPRRKGFVFPYAWRRSPERDWDLFVPLWYRSREFETTGSTASVARGAGR
                     GGASVFFPLYWAGRNPERDYRCVLPLYFHYGEPGRRLNVLAPLWMTYDSPAGKKFRMF
                     FPLYWRFLRKGEEKSSTNDVRSVDRDIVVLGPWYRVTVTRPGGRSRTVGLAPVFSSTV
                     SGPNDRYFEILGGLFGRDIQSGLRRYRIFYFFYTAPRPGGPAAVPAAP"
     gene            complement(11800..12096)
                     /locus_tag="P5079_02750"
     CDS             complement(11800..12096)
                     /locus_tag="P5079_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRY28938.1"
                     /translation="MIEHIVLFNIRRSATEEQKGQMLLNLLGLREKISGIHSATAGEN
                     FSARAKGFTHGFVVRFISRSALEAYLPHPAHQEVVEKYIKPIAEEILVVDYETD"
     gene            complement(12143..12370)
                     /locus_tag="P5079_02755"
     CDS             complement(12143..12370)
                     /locus_tag="P5079_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28939.1"
                     /translation="MKELERDCLELRLAEQAILRQALEEKNARTRELEAEVRTLRSLL
                     SELAAAPRPQYAPAPAAAPRTRGAVPLRWKA"
     gene            complement(12610..13125)
                     /locus_tag="P5079_02760"
     CDS             complement(12610..13125)
                     /locus_tag="P5079_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY28940.1"
                     /translation="MESMMQNQAVWAEEFLGRVDLFEKCSPQDIHLLTLNMRKQSFDK
                     GDTILFQGIISHQLYFVVSGMVAVFSRKDKVTRFIANLEKGAFFGEISLVKNCAATAT
                     IKAAVDQTEIYTIEHDVIREIMERHPEVKSDLEEKVRERNAGRLEAFEKQKEELPSAA
                     PLSAQASAARY"
     gene            complement(13224..14015)
                     /locus_tag="P5079_02765"
     CDS             complement(13224..14015)
                     /locus_tag="P5079_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY28941.1"
                     /translation="MKKRATEVRKIIRINEDLCDGCSKCIPSCPEQAIRLVETPRGPK
                     ARLVKEFYCDGLGACLGACPTGALVIEERRAEVYDEEATLARIKREAPEMLEEHRRHM
                     REHEEKPSPARPPGVSACPSSRSALWDAPPASPAAHRQELKSELRNWPVQLHLVSPEA
                     PYWAGADVVFAADCAPFGYAGFHQDFLKGKVVAIGCPKLDDVEAYLDKVTRIFEAGPR
                     SVTVVHMEVPCCFGLVRLVREAQARSGKKIPVETAVIGVRGERLG"
     gene            14218..14835
                     /locus_tag="P5079_02770"
     CDS             14218..14835
                     /locus_tag="P5079_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HRY28942.1"
                     /translation="MRLWSSRALPFFSAALAALFLFSCSSTPKVRRTEVDEQIDLSGN
                     WNDTDSRLVAEEMVRDALARAWVSEFSAAKQTKPRVIVGTVLNKSHEHINTETFVKDL
                     ERELTNSGQVRFVASKEQRNEVREERVDQAQNASMETAKSMGQEYGADYMVKGQINTI
                     LDAAGKQEVKYYQVELELIDMLTNEKAWIGQKKIKKLVKYKKAKF"
     gene            14888..16324
                     /locus_tag="P5079_02775"
     CDS             14888..16324
                     /locus_tag="P5079_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28943.1"
                     /translation="MNRRRRSAVLLRGLIGSLTAAALLAGCASPRALMRDMQTLITQE
                     QYPAAAELVKNAKTSHYGAKNAFLYYADLGMTLHLAGKYAESNAAFESAKKIATDLFT
                     KSVSKEAATFLTSDNARPYAGEDFERALIHVFCALNYVFLGEENEALVEARQADFLLT
                     KLQTDYGHKNVYTEDAFIHYLSGMVYENAGETNDAFIAYRKALDAYVRYRKDYGTAEP
                     PALIQAALRTAAKLGFTDEVREIKNRWGGTVPGTPPTGDGEVVVFHYNGLAPYKTDSF
                     FEIGFIAGWAYVEAENPQGEDREKMEQAGAAVRSLLADDVVRVAFPKYVRQPYAVKSL
                     EVRAAEANASHSANLAEDIGAIAIKNLQDRIGRVRTKAIARSVLKFALSRKIAAEVEE
                     KRGAGAAWLTKKILQVASSATELADKRCWQTLPDQIGVASLTLPAGKHTLTLTFRDAA
                     GNTVLTREMKDVDVRPGKKTFLTIRTAH"
     gene            16344..17396
                     /locus_tag="P5079_02780"
     CDS             16344..17396
                     /locus_tag="P5079_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRY28944.1"
                     /translation="MKRSRIFAGLIALAVLSAAAAHAAKRPAWTQGEDPAYPNATYVT
                     GVGIGDDLDAARSNARAEISKVFQARVQQVTSDVQSETSGAVGGKRSPVESAQESEMR
                     TKVTTESLLEGVQISQTWFDKKKKKHYALAVLSRPKTLAALSGQITEKEEGIGAQMSI
                     GKAAPSAIEKARAYAQAYRLAQERDALAARRRAVDPVTVPDLPGTGNSTAEAENLLRQ
                     TLAQIQFTVEADGETPSRLKESVTGKITAMGFKVLDSTAPAATPMSPVIKIKCRLTIE
                     PFDRGNPSWKFFRWNGAFEMAESGKNGRVLASSTPAGAEGHLTEDTARTKTRISGEEA
                     LAKEAETQIGRYILGK"
     gene            17476..18021
                     /locus_tag="P5079_02785"
     CDS             17476..18021
                     /locus_tag="P5079_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRY28945.1"
                     /translation="MSRISFIKLAALLVAGTLAVGCAAKKPEWIMKGSGAFKKDQKVI
                     YGVGVAEGVQSEALRRMTADNRAIAEISKQLSTISTSLMRDYMSSTAVPPEQKGSEEQ
                     YIENTVKTFTSNTVSGVKVTDRYQEKNGTLYSLASLNLDDLKMLAGEVKDLSESVREY
                     IKANAEKAFDKLAEEESKHAQ"
     gene            complement(18111..>19623)
                     /locus_tag="P5079_02790"
     CDS             complement(18111..>19623)
                     /locus_tag="P5079_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY28946.1"
                     /translation="DDYILIADDREPLPARLKVVERQAHQALRRRNVEESLHYRVELE
                     KLIAGLSANFVNLDPDDFDAAAHRALQAIGEFAGVDRSYIFLLAPDGRHASCTHEWCA
                     PGIERLISTMQNLSTEDFPAWTKALKNFETIHIPRVSEMPPEAASEKKTLEGQGVQSV
                     IVVPLMLGRTLLGFVGFDAARQVKEWDEDDIRLLKMVGEILANSLERKRNEESLMEGE
                     ERFRRLVENAADAFFICDSSGNIQDVNQRACKSLGYTRGELLSMNMRDIESPPFLDPI
                     LKGWGNFLQSSALTLEGIHCRKDGQKFPVESRVGFFDFGGRTLVLALVRDITLRKRAE
                     DRLRRLNETFLSLGTDYDGNIQNLTESCGILMRAACALYNRLQDGMLFSAGRWNTPPD
                     YNPTDKPDGHICYDVISKGRDGGLFYVRNLPETPYSKSDPNVAAFGLKTYVGHPVSRN
                     GKPVGSLCVVYRDDVELDDQDRRLMEIFASAISIEEERRLTSPMAVWAASKGN"
BASE COUNT         3832 a         6650 c         5832 g         3309 t
ORIGIN      
        1 cttcgggacg tccgctttca tgagaagcac cagagactcc acctcctccg ccgtcaaaga
       61 acggtctatc cagggataga ggacggcctc ctccttgttg ttgtggtccg aaagcacgtc
      121 caacaacggc gtctcccact gtccggaatt gccgttcagc aggtcggccc cgatcttttc
      181 caggtccagc ttgatctccc ggtgttccat ccgcatcacc tgggtcggac cgctcaacgc
      241 catccccgtc tccttttcaa aagaagggaa aaggaggtcc tcctcccagc ggatatgccg
      301 aaggagcccg gaccggaacg cccgaaacag atccatcgcc ccggacaggt ccttcttccg
      361 gacgtcctgg aaccgccgga agatatcgtc caggcggtcg tggtccgcgc ccatgaattg
      421 attgacagtc tgcataagac ctcccgtgaa aagtttcctc cagtatagcc gggacgggac
      481 aaaaacgtct atgacggccg tcatagggtc gtccgaaaaa gatccggccg gcccgcgacc
      541 cgtcgaatcc gcgtttgccg gtggaaaata tgtaaaatag acccatcgtc cccggtccta
      601 tccgaagagg gagctcggtc atgtcacaaa caatttccat tccgcgatga cgccgcaacg
      661 cgcctccaaa agttcccccg gcgccccgct tctcgctctg gcgatggcct tttcccttct
      721 ctccggcgcc gcccgggccg ccgatctgaa aaagggcgat ccgtgcccct ccttccaact
      781 cgtccccctc gccagcaccg cctcggccgg aatccctctg gaaaaactcc ttgaaaacgg
      841 ggaaccggtt ttactttcct tctttgcggc cgggtgcgtc ccctgtttga cggaaatccc
      901 cacgctcgaa tacatcgccc agacccaaaa cgtcgacgcc tacctcatca gcgtggacag
      961 cgccccggcc gaccgggtga aatcgctggc cctgaaacac aacttcaccc tccccgtcct
     1021 gctcgacccg gcggggaaac gcgcgggact cccgtacggc gccgtccccg gcccccaaac
     1081 gacctcccct caactgttcc ttctgtcgcc ggacaaaacc ctctggtggc gaagccagga
     1141 ctccgcggcc ggactggaaa aggaactgac gaaccagatc cgggacctga acgaggccct
     1201 cgcccgacgc gttccggccg cgccgatctc cctggattcc gacagttttc tgctggtctt
     1261 caccaacagc gccaacgggt acctgtcccc ctgcggctgc gccgacctgc cgttcggcgg
     1321 cgtctcgcgc cggtccactt ttttcaaagg cctgcggaaa aaattcccgg acccctccct
     1381 cctgttcctc gacgccgggg acaactacgc cctgcgcgcc ccttctttac aaagcgtcat
     1441 ggacgtgctc gacgtcctcc gttacgacgc catggtcttg ggcgaccaag acattctgct
     1501 cagcggcaag gacgcccgaa aaacgctttt caacagaagc cgtcccttcc tggcggccaa
     1561 cttcgaggtg tgcgacgacg ccggatgcga cgcccccttg ccgcataaaa ttttcaagcg
     1621 gggcgatctg cgggtcgccg tcattgcggc catggacccc gacatctaca ccctcgcccc
     1681 gaagacctgg aagaagtggg tccacctcac ggacctccgg aaaaaactcc aggagtcgct
     1741 cccttccttg agggaaaaca acgacgtggt catcctgctt tcccacagcg gcctggagac
     1801 ggactgctcc ctggcggaaa acatccccgg catcgatatc gtggtgggcg ggcattccca
     1861 aacggccctg aaagacgcca tccgggtcac caacctgaat tccggcggac acgccttcgt
     1921 ggtgcaggcg ggcccgaacg gacaatatgt cggcaaggtg gtggtccgga tgcaaccgga
     1981 caagacggtg gaactgacgg gttacgaact ggtccccttg acggacgcca tcgaggacga
     2041 cgcggaggtc ctgaaacttc tgccccaata ggaccgacgg ctttccctca aaaaagaagc
     2101 ccctctgtgc ggaggggctt tctctttttc aaacacggcc gcgaagagcg ccgttacgcg
     2161 cagggaccgc accctccggc gggcttttcc tcctccgggt gcctgttcca cgggagtttg
     2221 gcgaggagct cggccaggcc gcatttgccg ctgatccccg cgaacaccag gccggccccg
     2281 acgaacacgg ccacccatcg caggggcacg atcaacccgc cgaacaacgc caacgtcccg
     2341 gccacgatct gcacctgccg caggaggggg atcggtccgc gacgccgctc cacggccaga
     2401 ccggcgtctt tccacgcctc cagccctccc tgcacggccc ggacccggtc gaaccccgcg
     2461 tttttcaata tcttcagggc ctgcgcggtg cgaacgccgg tctggcagag gaccgccatc
     2521 tcccggtctt tcggaaaaga cggcaactcc gccgccaacc ggccgagcgg cacgttccgc
     2581 gtcccgccga tatgctcgta acgatactcg tcttcctccc gcacatccac caccaccgcg
     2641 ccgccggcca gacgtttctt caatccgacg gccgtcaagc ggccgtcggg gaggatttcc
     2701 gtcgtcgggc ccgcgcccgt cccggcgctt ttccggcggc cgaacgacgg acaggccagt
     2761 atcccgcccg tcaggttgaa gacgtcgccg aaaccgttct gctgaaggat gcgcgcggcc
     2821 agataaccgc ggaaccccac acggcaatac accaccgtct ccttcttcgg gtccagttcc
     2881 gaaatcctct cgcgcaaaac gtcgatgggg atgagccggg cggactccag gtggccgccg
     2941 tcgtattcgg ccggggtgcg cacgtccacc acctgcaccg gttcgcccct ccgctgtttt
     3001 tccaataact gcccgcaagt gacggtcttt atctcgcccc gcaccgcgtt ggcggccacg
     3061 aagccgccca tgatcaccgg gtctttggcc gacgagaact gcggcgcgta cgccaggtcc
     3121 agattctcca ggtccgccgc cgtcatgcgc gcggaaagcg ccgtggccag aacgtccgcc
     3181 cgtttgtcca cgcccttctc gccgacgatc tgcgccccca gaagacggcc gctgccttcc
     3241 tcaaagacca gtttcatgtg caggggctcc gcgcccggat aatagccggc gtggtcgagc
     3301 gaatgcgtca gcgaaacccc cacgcgcaag cccgccgcgc gggcttcctg ttcggagagc
     3361 cccgttttgg cggcggagat ccccagggac tccacgatgg ccgttcccag cgcgccgggg
     3421 aacgtcatcc ggccgccggc ggcgttggcg ccggccacgc ggccctgttt gttcgccggg
     3481 ccggccaacg gcatccggat tttccgcccg gtgaccagct ggaccacctc caccgcgtcg
     3541 ccggcggcgt agatgtccgg atcggaggta cgctgcctct cgtccacgac gatgccgccc
     3601 gatgggccga tggccaaccc ggcctcctcg gccagtttca gttccggccg cacgccgatg
     3661 gagaggatcg ccatgtccat ggggatcctc ttcccgctgg ccagctcgat ctccgtcgcc
     3721 aaggggtctc cgtgaaaggc cttcagggcg tcgccgagca ccagcgtcag cccggcctcc
     3781 tccagatgcc gggccacgga gtcggccacg tccgggtcga acggcggcaa cacctgcggc
     3841 atcctctcca ccaccgtcac gtccaacccc agccgtttca gcgcctcggc ggactccagg
     3901 ccgatgaaac cggcgcccgc caccacggcc cgggccggcc ggtgggaccg cagaaattcc
     3961 ttcaccgcgt cggaatcggg cacggtcttg acggtgaaaa tattcttcgc gtcgatgccc
     4021 ggcaacgccg gccgcaccgc gcccgcgccc ggggccagca cgagggcgtc gtaggcctcg
     4081 cggaatacgt cgccggtttt caggttcttc acttccaccg ttttcgcggc gcggtccagg
     4141 cggagcacct cgtgccccac gcgcgcgtcc acgcgatacc gcttccaaaa cgattccggt
     4201 gtctgcagga ggagcgaccg ccgttcctga atgtcccccc cgatgtaata cgggagaccg
     4261 cagttggcga aagacacgta cggcccccgc tcgaacatca cgatctccgc ctcttcattg
     4321 acgcggcggg ccttggccgc ggcgctggcc ccgcccgcca cccctccgac caccacgatc
     4381 ttccttttca tcgtttcccc ccggcgtccc gcccgctgaa aattccccaa tgtctcataa
     4441 catccgctgt aggaaaaagt tcccggcgct agccttgccg gtcggcggcg ccgcgcgcct
     4501 cggccttccg gcgttccatc tccgccgcca gacggtccaa ccgctcctcg cccaacagcc
     4561 gttcggccgc cgggaagagc cccgcctcct ctttcagaag atgcatgtcc agcagcgacc
     4621 ggaactggag catgtcgatc tcccattcct ttttttgcgc ggcgggggtc cgacgcatcc
     4681 gcctcaaaaa atgcgccagg tcctcatgct cctggacgat ctcccgggcg taacgcttgt
     4741 cgagcagcgg ttccgcctcc aaagccggta aaaaaagctc gtcttcgaac caggcgtgcg
     4801 ccaagatgat cctctgcagc gtttccaaga tgagcgcgaa ccgaggattc cccagcgtga
     4861 acccttccag tgtcttccgg atcatcttat ggtctttcaa gagctggtcc gtggctttcg
     4921 gcatagcgtt cctttcggcg atcgggtccc ggcccgacga tttattttca caaaactgct
     4981 aaactgaaaa acatgaacta cgacgccatc gtcatcggag gcggcatcac cggcgccggc
     5041 gtgctgcgcg atctggccct gcgcggcctc aaagccctgc tggtcgaaaa ggacctcccc
     5101 ggccgcgcca ccaccggcgc ctcctcccat ctcatccacg gcggggtgcg ctaccttctc
     5161 tacgaccgcc ccaccacgga aaccaccgcc tgggattccg ggcacatcgt ccgcatcgcc
     5221 cggcctctcc tcacgcgcct gcccatcgtc tggcccgtgt atgcggacca cgcgcgcgga
     5281 cggaccacca tcgagaccct tctggaaagt tacgatcgtt tccagccgct gaaaggcgga
     5341 ctgccgcacc tgcggctgaa cgccgaggaa accgcgcggc tcgtccccca actcaaacgc
     5401 cccggactgc gcggcgggct cgtgttcgac gagtggtggg tggaccccgt ccgactcgtc
     5461 gaaaaaaatc tcgacaacgc ccgggcgaac ggcgccgagg tgcgcgaaaa aaccgccgtg
     5521 accgggctgg tcctcgccgg cggcgccgtg aagggcgtgg aggtcctcac cccccggggc
     5581 gagcgggaaa ccctgcgcgc cgacctcgtc atcaacgccg ccggcccgtg ggtggacaag
     5641 gtggcccgcc tggcggggct ctccatcccg ctccgcctgc aacagggaac gcacctcgtc
     5701 tacgacggaa acgaagccat tctgcgctcc cccgccggcc ggttcggcct gttgctggag
     5761 gccgaagaca gggaacgcta cgtctttgtg atccccggcg gggacatgac cttggtgggc
     5821 cccaccgaca tcacctcgcc ggacgacccg gaccatctgt cgccccagcc ggcggaaacg
     5881 gcgcagctgc tgggcacact ccgccgctat ttcgattcgt tcccggaacg ctgttccaaa
     5941 accatcgtgg cggcccgccc gatcctgggt caaacgggca acgccaaact tctctctcgg
     6001 gagttccagg tgctggacca cgcggcccgc ggcgaacggt cggggttcct gaccatcgcc
     6061 ggagggaaaa tgtcggattt tcgtctgatg ggagaggaga ccggcaacgc cgccgttcgg
     6121 ctgttgaaaa aaggcggttc cggacggacg cattcgatcg cgctggacgg ctcgtccgtg
     6181 tcgacggaag gctccgcgga ccggccgacg catccgctca cgccgttcct gagacgacac
     6241 ccccgcctga gggaactgca cgccctggcc catctgggcg tggggctcgc cggccacctg
     6301 gcgcgacggc tcggcgggaa aacgcgcgac gacaccgtcg acgatttcac ccgccgctac
     6361 aacgcccccg cctcagggct ctaagacctc cgacgacgcc ggcttttccg gcgactcggg
     6421 tccgtgcgcg aacacgcgcg gcatctccca caacgagaaa aacaccgccg ccacgatcaa
     6481 cacgaaagcc accacgtagt tccaccgcat cggctccctc agataaaaca aacaaaaaaa
     6541 gatgaacacg gcgacggaga tgacttcctg gatgatcttc agctgcggcg cggtgaacgt
     6601 cccgtggccg atccggttgg ccggcacctg cagacaatat tcaggaaacg cgatcagcca
     6661 gctgaccaag atgaccgcga tgagcggata cgccttgtac ttcagatggc cgtaccacgc
     6721 caccgtcatg aaaatattgg accccagcag caacagaacc gtcttcatgg aatgccctcc
     6781 gtccggcagg aactgtccct attttatctg tttttgaaac gccccggagg ccccgttcct
     6841 aaaacaattt ccatggcggg gccgccggcg acgccgccct gatatataat gtggttgctg
     6901 gacaggcatc gccgcggagc atgcctcatc ccatgaacga ggtgcccgcc catgcctgaa
     6961 ttcagtccga ccgcccggca gaacccctcc atcctcgtcg tcgaagatct tgaatccacg
     7021 cgcgaattcg tctccttcat cctccgcaaa cagggatatc atgtcctgct cgccaaggac
     7081 ggcaaaaccg gcctctccct ttccgtggac aaacagccgg accttctgct ggtggacatc
     7141 ctcctgccgg ggatggacgg ctactccctc tgctcgcgca tcaaacagga cccggccctg
     7201 aaacggcaga agatcatcct tttctccgcg ttgaacgccc aagccaacaa gctcaaggcg
     7261 gaagaatccg gcgccgacgc cttccttgaa aaacccgtgg aaccggcccg cctcctgtcc
     7321 gtgataaagt ccctgctctc cccgtcctga aaaatcagaa aaaacagttg acagtcccgg
     7381 gacagcggcc tataatggac gtggttcatc actaaaacgc cgagggaacg gaggcccctg
     7441 tgagggcgac ggtttcatcg gcgtttgtca ttcggaacat cgcgtttttc ccggcggcct
     7501 tccttttaat aggagggctt tttgttttcc aggccccgct ggccgccctc gaaaggcccg
     7561 ccctcgtctg gaacgtggcc gccgaccgcc tggaaacctc cccccgccaa ccggtgaaag
     7621 cgagcgggcg cgtcgtgttg gagatcaagg actcccgcgt ggaggcggac aacgtggcgt
     7681 tgctgccgaa ccttaaacgg gcccaggcct ggaaaacggt ctcccttcaa aacgaatcgt
     7741 tcctggtgcg gacggaatgg atggcgttgg actgttcgca gagcggcgcc ccgtgcgcgc
     7801 tctacatcga cccactcggc aacctgccgg gagaacgacg gacgcggacg cccaaattct
     7861 ggagtttctc gggcgagccg gcacgagccg gcggcgagga gacctggcaa tgagtcccct
     7921 gaaaaccccg ctgtcttccc gtttcaagtt cgggttctgg ctgggcggca tctccctggc
     7981 ggcggcgatg tgcctgttgg ccacctcgcg tttcagcgaa tccggctcgt tggccggcga
     8041 gaccgcacgc ctgatggtga ccagcggctc cttctcccgc ggcgtcaccc tgtacgacgc
     8101 cctcaaacag gcgtccctct cccccctgct catccagcag atcggcgatt ccctccaaag
     8161 gagcatcaat ccccggtcgt tcaagaacac cgacacctac gaactcgtgc agaccgcgga
     8221 cgccgctttc caccacctca ccatcacccg gggattgaaa cagtacatcg tctccctcga
     8281 tccttcccag cgactcgcgc tctacctgca ggagatcccc gtccacactc aagaggagat
     8341 ctctgccggg tccctcaagg gctccctctg ggagtccatg agttcccgcg ggatgccgcc
     8401 ggaactcatc atgcagttcg ccgacatctt cgcctggaac atcgattttc tgacggaacc
     8461 gcgggacggg gaccggttcg ccctggtgtg ggaaaaaggg accacgccgg acggcgaagt
     8521 cacccagcaa aagatcttgg cggccctcta cgagggccgg gccctcggcc ggcacctggc
     8581 cttcctgcac aacggcgaat actacgacac cgacgggaac tccatccgca aggccttcct
     8641 gcacgcgccg ctcaacttcc ggcgcatctc ctccggcttt tcacggcggc ggttccatcc
     8701 ggtgctgcgc tatttccggc cgcacctggg catcgactac gccgccccgc gcggcacccc
     8761 ggtggtgagc gtgggcaacg ggacggtggt gttccgggga tggaaacgcg cctacggcaa
     8821 cttcgtgcag atccggcaca acggcgccta caccacctgc tacggccacc tttcccgttt
     8881 cgcgcggaac ctccggcgcg gcgcccgcgt gagccagggc caggtcatcg ggtacgtggg
     8941 cagcacggga tggtccaccg ggccgcacct ggatttccgc gtgctcaaga acggccgttt
     9001 cgtcaatttc atcaagctga agttcccgtc ggacaagcgg atccccgcct cggaaaacga
     9061 ggcgtttcag gaactcggcc ggcagcggcg ggaagagatg gaagattttc tgaaggagca
     9121 cctggcggcg gaataggcgt tccgcccgga aacgttacgg agcggcggga acagcggccg
     9181 gccctccggg ccggggagcc gtgtagaaga aatagaaaat ccggtagcgc cggagaccgc
     9241 tctggatgtc ccggccgaag aggcctccca aaatttcgaa ataccggtcg ttcggaccgg
     9301 acacggtgct ggaaaacacc ggcgccagac ccacggtgcg gctgcgcccg ccgggacgcg
     9361 tcaccgtcac gcggtaccac ggccccaaaa ccacgatgtc ccggtccacg gaccgcacgt
     9421 cgttcgtcga cgatttctcc tcccccttcc gcaggaacct ccaatagagc ggaaaaaaca
     9481 tccgaaactt cttcccggcc gggctgtcgt aggtcatcca cagcggcgcc agcacgttga
     9541 gccgccggcc cggttcgccg taatgaaaat aaagcggcag gacgcagcga taatccctct
     9601 cgggattccg gcccgcccaa tacaacggga agaacacgct cgccccgccc cgccccgcgc
     9661 cccgcgccac gctcgccgtc gacccggtcg tctcgaattc ccgcgaacgg taccacagcg
     9721 gcacgaacag gtcccaatcc ctttccgggc tgcgccgcca ggcgtagggg aaaacgaatc
     9781 ctttccgtcg gggcccctgc cgttcccacg ccgccagcgc gatgccgcgc ggcgcccaga
     9841 agttccggaa atccgcccgg cggatatatt tgtagagtgg gaaaaattcg tgttccaggt
     9901 tttccccctc ccgcgccacg ccgcccagca ggagatattg cagatgcgat cgcggccggt
     9961 cggtgcccgt gccgcccctc gaaagataca ccagcagggg cggcacgatc catagcccgt
    10021 cgcccttttt gtccgccgaa taccaataga acgggaagag atagccgccg cgttcttccg
    10081 gcgaaaaatc ccggccccag agagggaaca gcccgtaggc gcgccgctcc tcccgccgcc
    10141 agcggtaata gggaggaacg atggtcagac ttttcgacgg cgtggcgctg ccccaataca
    10201 ccgggaagaa aatgaaccgc ttcgactcgg cgctgcggta ccaatacgcc ggcaacacga
    10261 tcccctcccg ggacagcgcc gagcgccgcg tccaggccag cggcgaaaaa aacagccggc
    10321 ggcgggcgtt gtagttgtaa tggaaaagcg ggaagagcgt tgtggaaccg cggtccgcct
    10381 gatggtcccg ccaataaagg aaaagcaggc tccaggtgcg gcggtcccgg gtcttcttcc
    10441 acaagaacgg cggaacggcg gtgagggtgc tgtccccgtc gcgagtgcgg gcatagaacg
    10501 ggaaaaggac atccacgccc cagtccggcg gacgccgaaa gtgccagtag agcgggaaga
    10561 aaacgcgggt gcggtcgccg ttgtcggcgt tcctccagtg gtagagatag gaccaccccg
    10621 acgacgaccc ctccttccgc cgccacagcg ccagccagga aacgaggtgg gccccgtccg
    10681 ggtcggacca gtagccgtag agcgggaaga gcgtctggaa atagtcctcg ttctcccggc
    10741 gctgataaaa attcaggatc tgcgtcttct cctcaaaacc gtcggcgctg cggcggagat
    10801 accacaacgg aaacacgccg gagagccgaa ggtccggatc gcgggaataa aagtagaggc
    10861 ccaggtggcc ggcgcgctgg tcgtcgttga cgaaagagct gaacggcagg gtgaggaagc
    10921 tccggctttt ctctccgcgg gaatagaggt acacgggcaa aagataatgg gatcggcgcg
    10981 cgccccagcc cgccccggcc aacgggaaaa ggcccatgtc cgtctggggc ggggccgtcg
    11041 gggcggaacg tttccagtaa aagggggcca gcacgtccag cttgccgccg gggttccgga
    11101 accgccaata ggccgggaaa aaaacgcgcg tgcgcacgcc cgggtcgtcg aaccacgcgt
    11161 aattcagaag gaagccgagt cgccggtcgc cgcggcggcg cgaccagccc cacaacgaca
    11221 cctggtccac cttttgcctg tccccgctgc ggtaatcgta aaaaaggaac ggcggcaggg
    11281 tggtgacccg cccgccttcg gcgcggatct tctgccaata aaacggaaag agcgcggtgt
    11341 gggtgacgcg gggatcggaa tgccggaaga aaaggcccgc caccatccat ttttccgtcg
    11401 gtcggcggta ggagaagacc agcggcaggg acaccagcag gctccggtcc gactcgcgcc
    11461 agcggtaaaa aacgggcagc atcaatttat aggaactgga ccggtcctga acgttccaat
    11521 agagcatgga aagcatcagg agccgccgct cttcttccgg gtccgcctcg taccaaaaca
    11581 ggggcggcag ccgcacgggc cgttttttcc aggaggccga ggcaggagat gcgccggtcg
    11641 aaccggaaac gcccgtctcc tccgcccgca gaccggacag gacggccgac gacacgacgg
    11701 ccgcgatcag aaaggtccga aaaagggatt tcaactgtct agcgggaaat ggggtctgaa
    11761 aaggaagcaa ggcgggcacg acggtgcgac ggaaacgggt caatccgtct cgtaatcgac
    11821 gacgaggatc tcctcggcga tgggtttgat gtatttctcc accacctcct ggtgggcggg
    11881 gtggggcaaa taggcctcca gcgcggaccg ggagatgaac cggaccacga acccgtgggt
    11941 aaagcccttg gcgcgggcgc tgaaattctc gccggccgtg gcgctgtgga tgccggagat
    12001 cttctcgcgc aggcccagaa ggttcaagag catctgcccc ttctgttcct ccgtggcgga
    12061 ccgccggata ttgaacagga cgatgtgttc gatcatgacc gtctcctgac tttacaaaag
    12121 tcgaaagccc ggagcaaacg gttcacgcct tccaccggag aggaacggcg ccccgtgtcc
    12181 ggggcgcggc cgccggcgcc ggggcgtatt gcggccgggg cgcggccgcc agttcggaca
    12241 gcaggctccg cagcgtccgc acctcggctt ccagttccct tgttctggcg ttcttttctt
    12301 ccagggcctg ccggagtatc gcctgctccg ccagccgcag ttccaggcag tccctttcca
    12361 gttctttcat gacgtgtctc ctttcttttt gtgtctccct aaaataaaaa acccattgtc
    12421 cgtcggacaa tgggcgcttt gcgttgcttt cggggaatcc ggcttgctta cttcatgtga
    12481 cccgtccaga ccgcaaaacg cccccgaccc gtgtggaccc aaaggcccat cgggcgagac
    12541 gcggtaatga atttgcaggt ctggttcttt ctcatatctc tttcagtata accccggttt
    12601 ctgaaaaaat caatacctgg cggccgacgc ttgcgccgac agcggcgcgg cggagggcaa
    12661 ctcctccttc tgcttctcga acgcctccag ccggccggcg ttccgttcgc ggaccttttc
    12721 ctccaggtcc gacttcacct ccgggtgccg ttccatgatc tcccgaatga cgtcgtgttc
    12781 gatggtgtag atctccgtct ggtccacggc cgccttgatg gtggcggtgg cggcgcagtt
    12841 cttcaccagc gagatctcgc cgaagaacgc ccctttttcc agattggcga tgaaccgggt
    12901 gaccttgtcc ttgcgcgaaa aaacggccac catgccggaa accacgaaat agagctggtg
    12961 cgagatgatg ccctggaaaa ggatggtgtc gcccttgtcg aaactctgtt tgcgcatgtt
    13021 gagcgtcaaa aggtggatgt cctgcgggga acatttctcg aacaggtcga cccgccccag
    13081 aaattcctcc gcccacacgg cttggttctg catcatggat tccatttttt cacctcttcg
    13141 ttaggcttgc ggccaggacc gccggctcaa ccgtatcatt atagcatgtc ggggcggggg
    13201 tgtgccgaac taaaatgccc ccctcaaccg agccgttccc cccgcacacc gatgaccgcg
    13261 gtctccaccg gaatcttttt gccggaccgc gcctgggcct cccgcaccag gcgcaccagc
    13321 ccgaaacagc aggggacttc catgtgcacc accgtgacac tccgcgggcc ggcctcgaag
    13381 atccgcgtca ccttgtccag gtaggcctcc acgtcgtcca gcttggggca cccgatggcg
    13441 accacctttc cttttaagaa gtcctggtgg aacccggcgt aaccgaacgg ggcgcagtcg
    13501 gcggcaaaaa ccacgtccgc cccggcccag tacggcgcct ccggcgacac cagatgcagc
    13561 tgaacgggcc agttccgcaa ttcggatttc agttcctgcc gatgggctgc cggagaagcg
    13621 ggcggcgcgt cccacagggc cgatcggctg gacgggcagg cggaaacgcc gggcggccgc
    13681 gcgggcgacg gcttttcttc gtgttccctc atatgcctcc ggtgttcctc cagcatttcc
    13741 ggggcctcgc gcttgatccg cgccagggtg gcctcttcgt cgtacacctc cgcccggcgc
    13801 tcctcgatca ccaacgcccc cgtggggcac gcccccagac aagcccccaa accgtcgcag
    13861 taaaactcct tgacgagccg ggccttcggg ccccggggcg tctccaccaa ccggatggcc
    13921 tgttccggac aggacgggat gcatttggag cagccgtcgc acaaatcctc gttgatgcgg
    13981 atgatcttcc ttacttcggt ggcgcgtttt ttcatgggtc tcctcccgga gaaagtcgcc
    14041 tgcctttccc ttccattcta cccttccgcg gttcaggcac ttatgacggc ggtcatcctt
    14101 tcaaggaact tcgggtctct ggaattcggg cccgaaaaat gctacttctt ccgtaagcag
    14161 cacagacacc ctcgcattca ctgccaacgg atccgacatc gttcaaagga gaaaaacatg
    14221 cgtctttggt caagccgagc cctgcctttt ttcagcgccg ccctggcggc cctgttcctc
    14281 ttttcctgca gttccacgcc gaaagtgagg cgcaccgagg tggacgaaca gatcgacctc
    14341 agcggcaact ggaacgacac cgactcgcgg ctggtggccg aggagatggt gcgcgacgcc
    14401 ctggcgcgcg cgtgggtgag cgaattctcc gcggccaaac aaaccaaacc gcgcgtgatc
    14461 gtgggcaccg tcctcaacaa gagccacgaa cacatcaaca ccgagacctt cgtgaaggac
    14521 ctggagcggg agctgaccaa ctccgggcag gtgcgtttcg tcgcctccaa ggaacagcgc
    14581 aacgaggtgc gcgaggagcg cgtcgaccag gcccagaacg ccagcatgga aaccgccaag
    14641 agcatgggac aggaatacgg cgccgactac atggtgaagg gccagatcaa caccatcctg
    14701 gacgccgccg ggaaacagga agtgaagtat taccaggtgg agctggaact gatcgacatg
    14761 ctcaccaacg aaaaagcctg gatcggccag aagaagatca agaaactcgt caaatacaag
    14821 aaagccaagt tttaattttc ccccttcact ccacgatcgc ccctccgaga ggttgcagga
    14881 ccgccgggtg aaccgccgcc gccgttccgc cgttctcctt cgcggtctca tcggctcttt
    14941 gacagccgcc gcgctgctgg ccgggtgcgc ctcgccccgc gccctgatgc gcgacatgca
    15001 gacgctcatc acgcaggaac aataccccgc cgccgccgag ctcgtcaaga acgccaaaac
    15061 cagccactac ggcgccaaaa acgccttcct ctattacgcg gacctgggga tgaccctcca
    15121 cctcgccggg aaatacgcgg agagcaacgc ggcgttcgag tcggccaaga agatcgccac
    15181 ggacctcttc accaagagcg tctccaaaga ggcggccacg ttcctgacga gcgacaacgc
    15241 gcggccctac gccggcgagg atttcgagcg ggccctcatc cacgtcttct gcgcgctgaa
    15301 ctacgtgttc cttggcgagg agaacgaggc gctggtggag gcccgccagg cggacttcct
    15361 gctcaccaag cttcagaccg actacggcca caagaacgtc tacacggaag acgccttcat
    15421 ccactacctc agcggcatgg tgtatgagaa cgcgggagaa accaacgacg ccttcatcgc
    15481 ctaccgcaag gcgctggacg cctacgtccg ctaccggaag gactacggca ccgccgaacc
    15541 gccggcgctg atccaggcgg cgctgcgcac cgcggcgaaa ctggggttca ccgacgaggt
    15601 gcgggagata aagaaccgtt ggggaggaac ggtcccggga acgcccccca ccggcgacgg
    15661 cgaagtggtg gtgttccatt acaacggcct ggcgccttac aagacggact cgttctttga
    15721 gatcggtttc atcgccggct gggcctacgt ggaggcggaa aacccgcagg gggaagaccg
    15781 ggaaaaaatg gagcaggccg gggcggcggt ccgctccctc ttggcggacg acgtggtgcg
    15841 ggtggcgttc cccaaatacg tgcgccaacc ctacgccgtc aaaagcctgg aggtgcgggc
    15901 ggcggaggcg aacgcctcgc attccgctaa cctggcggag gacatcggcg ccatcgccat
    15961 caagaacctc caggatcgca tcggccgcgt gcggaccaag gccatcgccc ggtccgttct
    16021 caagttcgcc ctgtcgcgga agatcgcggc ggaggtggag gagaaacggg gcgccggtgc
    16081 ggcgtggctg acgaaaaaga ttctgcaggt ggcctccagc gccacggaac tcgccgacaa
    16141 gcgctgctgg cagacacttc cggaccagat cggcgtggcc agcctgacgc tgcccgccgg
    16201 gaaacacacg ctgacgctga ccttccggga cgcggccggc aacacggtgt tgacgcggga
    16261 aatgaaagac gtggacgtgc ggcccgggaa aaagactttt ttaacgatcc gaaccgccca
    16321 ctaggcggac aaaggagcca ctcatgaaac gctcacgcat cttcgcagga ctcatcgccc
    16381 tcgcggtcct ctcggccgcg gcggcccacg ccgccaaacg gccggcctgg acgcaggggg
    16441 aggatccggc ctatcccaac gccacctatg tgacgggggt gggcatcggc gacgacctcg
    16501 acgccgcccg gtccaacgcg cgcgcggaga tatccaaagt cttccaggcc cgggtacagc
    16561 aggtcacctc cgacgtgcag tcggaaacct ccggcgccgt gggcgggaaa cgctcccccg
    16621 tggaatccgc ccaggagagc gagatgcgca cgaaagtgac gacggaaagt ctcctggaag
    16681 gcgttcagat ctcccagacc tggttcgaca aaaaaaagaa gaaacattac gccttggcgg
    16741 tcctgagccg gccgaaaacc ctggccgccc tctccggaca gatcacggaa aaagaggagg
    16801 gcatcggcgc gcagatgtcc atcggcaagg ccgccccttc cgccatcgag aaggcgcggg
    16861 cctacgcgca ggcgtaccga ctggcccagg aacgggacgc cctcgccgcc cgtcggcgcg
    16921 ccgtggaccc ggtgaccgtg ccggacctgc ccggaacggg gaactccacc gccgaggcgg
    16981 agaaccttct ccgccagacg ctcgcccaga tccagttcac ggtggaagcc gacggcgaaa
    17041 ccccgtcccg cctgaaggag tcggtgacgg ggaagatcac ggccatgggc ttcaaggtgc
    17101 tcgattccac cgcgcccgcg gccacgccga tgagcccggt gataaagata aaatgccggc
    17161 tcactatcga acccttcgac cgcggcaacc cctcctggaa attcttccgc tggaacgggg
    17221 cgttcgaaat ggcggagagc ggcaagaacg gccgggtgct ggcttcgtcc acgcccgccg
    17281 gcgcggaggg ccatttgacg gaagacaccg cccgcacgaa gacgcggatt tcgggggagg
    17341 aggcgctggc caaggaagcc gagacccaga tcggccgcta catcctcggc aaatagtttg
    17401 ctatcatcgg cgttatgggc ccggcaggca ccaaaaaaaa cgttttcacc caaatcgaat
    17461 aacggaggag gagtcatgtc tcgtatatct ttcatcaaac tggcagcgct tcttgtggcg
    17521 gggaccttgg cggtgggatg cgccgcaaaa aaaccggagt ggatcatgaa gggcagcggc
    17581 gccttcaaga aggaccagaa agtgatctac ggcgtgggcg tggcggaagg cgttcagtcc
    17641 gaagccctgc gccgcatgac ggccgacaac cgcgccatcg cggaaatctc caagcagctc
    17701 tccaccatct ccacctcact gatgcgcgac tacatgtcct ccaccgccgt gccgccggaa
    17761 cagaaagggt ccgaggaaca atacatcgag aacaccgtga agaccttcac ctccaacacg
    17821 gtctcgggcg tgaaagtcac cgaccgctat caggaaaaga acggcacgct ctactccctc
    17881 gcctccctca acctggacga cctgaagatg ctggcgggcg aagtgaagga cctgagcgaa
    17941 tccgtgcggg aatacatcaa ggcgaacgcg gaaaaagcct tcgacaaact ggccgaggaa
    18001 gagagcaagc acgcgcaata atctcctcac ttcaccgaaa taaaaaaggc ggggccgaaa
    18061 ccccgccttt tttatttgtc cggacgacgc gttgcggcgt ccgccgggcg ctaattcccc
    18121 ttggaagccg cccacaccgc catgggcgag gtgagccggc gctcctcctc gatgctgatg
    18181 gccgaggcaa agatctccat caaacgccgg tcctggtcgt ccaactccac atcgtcccga
    18241 tacacgacgc agagggaccc gacgggtttc ccgttgcggc tcaccggatg tcccacgtag
    18301 gtcttcagtc cgaaagcggc gacgttcgga tccgacttcg aatagggggt ctccgggagg
    18361 ttgcgcacgt aaaacagtcc cccgtcgcgg cctttgctga tcacgtcgta acagatgtga
    18421 ccgtccggtt tgtccgtggg attatagtcg ggaggggtgt tccagcgtcc ggcggagaac
    18481 agcatcccgt cctgaaggcg gttgtagagg gcgcaggcgg cccgcatgag gatgccgcaa
    18541 ctttcggtga ggttctggat gttgccgtcg taatcggtcc cgagcgacag gaacgtctcg
    18601 ttgagccgcc tcagccggtc ctcggcgcgt ttgcggaggg tgatgtcccg caccagggcc
    18661 agcaccaggg tgcggccgcc gaaatcgaag aatcccaccc gggattccac gggaaatttc
    18721 tggccgtcct tgcggcaatg gatgccttcc aaggtcagcg cggaggactg gaggaagttc
    18781 ccccatccct tcaggatcgg gtcgaggaag ggcggagact cgatgtcgcg catgttcatg
    18841 gagagaagtt ctccccgcgt gtatcccagg ctcttgcagg cgcgctggtt gacgtcctgg
    18901 atgttgccgg acgaatcgca gatgaaaaaa gcgtccgcgg cgttctccac cagccggcgg
    18961 aagcgctcct ccccctccat cagcgactcc tcgttgcgtt tccgttccag ggaattggcg
    19021 aggatctccc ccaccatctt caaaaggcgg atgtcgtcct cgtcccattc cttgacctgg
    19081 cgggcggcgt cgaaccccac aaaaccgagc agcgtgcggc cgagcatcaa cggcaccacg
    19141 atgaccgact gaaccccctg cccctcgagg gtcttctttt ccgaggcggc ctcgggcggc
    19201 atttcggaga cgcgcgggat atggatggtc tcgaagttct tcagcgcctt cgtccaggcg
    19261 ggaaaatcct cggtggagag gttctgcatg gtggagatga ggcgttcgat gccgggggcg
    19321 caccattcgt gcgtgcagct ggcgtgccgg ccgtccgggg cgaggaggaa gatgtagctg
    19381 cggtccaccc cggcgaactc gccgatggcc tggagagccc gatgggcggc ggcgtcgaaa
    19441 tcgtcgggat ccagattgac gaaattggcg gagaggccgg cgatcagttt ttccagctcc
    19501 acgcggtaat gcaggctctc ctcgacgttc cgccggcgca acgcctggtg cgcctgccgt
    19561 tccaccactt tcaagcgggc cggaaggggt tcccggtcgt ccgcgatcag aatatagtcg
    19621 tcc
//
LOCUS       DAOTFD010000024        19172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1892_length_19172_cov_5.083381, whole genome shotgun sequence.
ACCESSION   DAOTFD010000024 DAOTFD010000000
VERSION     DAOTFD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19172
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_19172_cov_5.083381"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..908)
                     /locus_tag="P5079_02795"
     CDS             complement(150..908)
                     /locus_tag="P5079_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY28947.1"
                     /translation="MNLEGKVSLVTGGTRMGREIALALARQGCRVAMLYRRSRSEAEA
                     TVRAVRNWGVEAMALRVDLARAEDLDRAVPSVEKKWRRLDVLVNMASMYERVPLGGLA
                     GKPSGRARPSWRRALNTDLRAAYLLSLRAAPLMRRTGGGRIINFSDWTAVSGRPRYKD
                     RLPYYVAKTGVKGLTESLALELAPAILVNAVAPGPILPPAGLSRSENRDVVRATPLRR
                     WGGPEEITKAVLFFIHSDFVTGECLRVDGGRHLC"
     gene            972..1838
                     /gene="metF"
                     /locus_tag="P5079_02800"
     CDS             972..1838
                     /gene="metF"
                     /locus_tag="P5079_02800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRY28948.1"
                     /translation="MKISELIKTVKPSFSFEFFPPKTDDDVAQLMQTARDLRTLDPTF
                     ISVTWGAGGGTQRKTIDTVSAIKTDLRVESMAHLTCVGASSWEISAVLDELQKKGVEN
                     ILALRGDPPRDHPYFVPHPDGFRHANELVAHIRQGWKFCIGVAGYPEGHVECPDKELD
                     LRNLKQKVDAGADFVVTQLFFDNTVYFKFVERARAIGIQAPILAGIMPVTNAHQIKRF
                     TTSCGATLPPALLSRLEAHPDDKAAVVQIGIDHAAQQCAELLRGGAPGIHFYTMNRSH
                     STTEILRRLRRS"
     gene            complement(1858..2553)
                     /locus_tag="P5079_02805"
     CDS             complement(1858..2553)
                     /locus_tag="P5079_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY28949.1"
                     /translation="MQIFREREEDLENLLNRGALWAVTYGDLMSYLMIFFLLLFVFSL
                     TGGTAFEEGISIIQESFGGQVNQARIDRAIRRSQEEIIAKDVRQAFVDQGLQQMTGVE
                     ISEDRIRITLREPILFASGKAFLKPEAFPILHGVAEIMRNQPHPLMIEGHTDNVPVGI
                     RNEYESNWQLSMARAYTVIRYFVDVEGLDPKRLSGVGYGEYQPVVPNTSTDNRARNRR
                     IDISLLRSAASAW"
     gene            complement(2553..3338)
                     /locus_tag="P5079_02810"
     CDS             complement(2553..3338)
                     /locus_tag="P5079_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY28950.1"
                     /translation="MDLMTIVGLLGGVTVIVFGMIQGQSWASFLNAHGVVLVLGGTIF
                     ATMINSSARELWDAFLAGLSLLKTQSTPSPEQSISQLVRLAQKARRDGAMSLKDEARH
                     VGDGFFSRAIQVSLSSPDQKTAYEILAKEINMIRARHREVGNLFRTMGLLGPMFGLLG
                     TLIGIVSVLKNMSDPRSVGPSMALALSSAFYGILFANLICVPVAGKLRMRSLNEAIAK
                     ELIVEGILAVVFTNEMPLFIQTRLHAYLQKRQNGESSPPEKKA"
     gene            complement(3352..4911)
                     /locus_tag="P5079_02815"
     CDS             complement(3352..4911)
                     /locus_tag="P5079_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRY28951.1"
                     /translation="MKRPLVVLSLLGALAAVAAAGSLVEDEIRFSRDLQTRIQVALDQ
                     LMGERKAIALVNVQLEISEEMKSRLDKALGASQPQKTVAVTEGAKWTSPATSAASSGL
                     PGFPAKVQKEQGTATVISVAPPTQAWDPAAFLSAGIAIKKVYVKVLLDKGLSAGAENT
                     ARAVVANFVGIDPARGDVIEIARIRMPSKFEEALQNPALLSPILQKAATLSVVMLTAM
                     CFFVLGLAALATVRRSVDRLASAVRTLQPVDTHAALTGPDGKQPLLTAELASPADRSA
                     PGMPERTLPHYPELTFEVPADKVAYLLEFLSNEEMETVALVLQHLTPSAKSAFLALLS
                     PEQAGEVVMAMSRPSVVDLEKLQRLKEAVEKHIRGSTGGVQAVVDMLNASGPGMRRQL
                     LESITQKDPALGTAVRERIFIFEDLDKLSQQELARVVSNVQLSDLAVAILKQSAELKT
                     RIKTVLPELTWKVLEEESQHRGPSSTDEKIGEAQDRVLRTVYKLIREGQISPSFVRLP
                     GRLGVPAGKSA"
     gene            complement(4908..5582)
                     /locus_tag="P5079_02820"
     CDS             complement(4908..5582)
                     /locus_tag="P5079_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY28952.1"
                     /translation="MATSARSSSQIDKERAMEFYKDARGYVNTYEFAAAVGSIHQAAQ
                     FDPSSVEIKAYDRRLERVLELFDDRIRQENQSHVFLEGLSKYIAAEYQTALLCLGSAL
                     GNWPLDDRLQRFIGEIEKETGYKLDRNNVRPTPELVSLKLEKASENFNENRLTEAMIL
                     CREVILLEPDNALAYKRMGSIHFAQGNFEQARDLWRQALALEPEDEELTRFVQLAEEK
                     MKRGKK"
     gene            complement(5834..7483)
                     /gene="groL"
                     /locus_tag="P5079_02825"
     CDS             complement(5834..7483)
                     /gene="groL"
                     /locus_tag="P5079_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452133.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY28953.1"
                     /translation="MAKQIKYSDEAHKAIMAGVDKLATAVKATLGPKGRYVVLDKKFG
                     SPTVTNDGVTIAKEIELEDPFENMGAQLVREVSSKTNDVAGDGTTTAVVLAQSILREG
                     FRNITAGANATHIKRGIDKAVEAVVAELKKVAKKVNLDAKEKAKEEITQIATISASDH
                     QIGELIAEAILKVGKDGVITVEEGKSADTSLEVVEGMQFDRGYISPYFVTDAERMEAV
                     LEDPLIIVTDKKVSAMGDLMPLLEKIVQQGKPFVLISEDVEGEALATLVVNKLQGRLR
                     CAAVKAPGFGDRRKEMLEDIAILTGATVVTEEKGFKLEKATVDMLGRCKRVVVEKENT
                     TIVGGEGDDKAIKARIAQIRKQIEESTSDYDKEKLQERLAKLVGGVAVIEVGAATETE
                     MKSKKFKVEDAMHATRAGVEEGIIPGGGVPLLRAQKALDGVKSADPDEQTGINIISRV
                     LEDPIRTIAENAGMDGSIAVDKVRNSKDSNFGYNAESGEFGDLVKMGVVDPVKVTRSA
                     LQNAASISGLLLTTEVLVTDIPEKKNNMPPMPHGGGMGGDF"
     gene            complement(7563..7856)
                     /gene="groES"
                     /locus_tag="P5079_02830"
     CDS             complement(7563..7856)
                     /gene="groES"
                     /locus_tag="P5079_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRY28954.1"
                     /translation="MKIRPLGDRVLVRPLEKEKQERSGIIIPDTAKEKPQEGEIVAAG
                     KGKVADDGKVIPMEVKVGDKILYGKYSGTEIKIDDKEYLIMHQEDILGILESK"
     gene            complement(8220..9242)
                     /locus_tag="P5079_02835"
     CDS             complement(8220..9242)
                     /locus_tag="P5079_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28955.1"
                     /translation="MNRYGMKKFVAFVLAMGLVLPACKSNNVSSKILGGADVATGTPT
                     LSTALAGLTSYISGVSSGGTNAVPQNGTIPSGTAAGLNPTVAGNTGVINGGSSQLSLV
                     TAQAFRYCNVAVSNLAAAPAAAAPSAAQSIAGYYLLALPADATTLTLDLNIGQALPGS
                     FHCFVTLQDASGNIGPIEDISITQVATVGTGDVQVSLSWDTKADIDLHVVDPNNVEVW
                     YGDKAPTTTKGTLDLDSNVGCPSTGISNENITWPTGTAVAGTYIVRVDNYNDCSLGTA
                     SNCVITVRVAGVTKTFAKTFAVNSDDAGGAGSGVEICRFTNAGAFSAPQPNLTRSIGT
                     KPAVCQ"
     gene            complement(9268..10119)
                     /locus_tag="P5079_02840"
     CDS             complement(9268..10119)
                     /locus_tag="P5079_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRY28956.1"
                     /translation="MRQSRLWLLFVVVAPLAAMAHGEPFFSERAVPIDTKIVEVGLAP
                     SQRQDKYKVANVEQKNTLRRVDLQARWSPKKQWETKLVLPYINNKVSGGTDSDTDSGL
                     GQTILAGKYAFRKNKAVQAQLEVPTGDQDKLLSEGFNLGVSYLAEGELSKDVHGIFNA
                     GYLFKSEFKSDFDVAGQSRIKVNPGEVLFASVAMERPYRGKILTGELYFARVGKTKSA
                     GKSVSNSDGNVINLACGLTKDWKEWRFKGALALGIGDEEVSSYDLTRPAGDWRLLFCA
                     TRRFQLK"
     gene            complement(10172..11764)
                     /locus_tag="P5079_02845"
     CDS             complement(10172..11764)
                     /locus_tag="P5079_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HRY28957.1"
                     /translation="MADPQGYLCLVLHAHLPFVRHPEYPDFLEEDWLYEAITETYVPL
                     LDMMERLAAEGVHYRLTMSLTPPLCNMLSDDLLLSRYQKKLHKLLELADKELHRTRSL
                     PAAFQETARMYHHNLNKAREIFEASHGRLLTRFKALQDAGNLEILTCGATHGYLPLMI
                     HEEARRAQIRVAVQDYHRHFGRAPRGIWLPECAYAPGVDKLLRENGISFSFLDSHGVL
                     FGVPRPRFGVFAPIYSPGGVAFFGRDMETAHQVWSAEQGYPGDYSYREFYRDLGYDLE
                     YNYIRPYLHEDGVRRNIGIKYYKVTGRVSLSEKEPYSPSHARETAASHAGNFLFNRER
                     QVEYLKGVMGRPPVIASIYDAELFGHWWFEGPSFLEYLLKKIHYDQQVFKTTTPKEYL
                     DKFPEQQIVEPEPSSWGDKGYHEVWLNASNDWIYPHLHICAERMIHLAKKHANAEGLL
                     KRALNQLARELLLAQSSDWAFLMTVGTARTYSDRRTREHVSRFLELFHQIQENRLRED
                     FVRSLEERDNIFPWIDYKVYQS"
     gene            complement(11871..12746)
                     /locus_tag="P5079_02850"
     CDS             complement(11871..12746)
                     /locus_tag="P5079_02850"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HRY28958.1"
                     /translation="MSSSSSSNVPSSTPPSQGFVETGPGLPRDYGVTRLVLIPRDPQW
                     MHAYWEVAPYTWQEAEKNFGSAVRTSGRAVLRLHCGDGASAKTFDVNIHLDARNWYIF
                     SPARGGLWHAELGMLLPDGRFVLLAISNEIRLPTGQVSDVIDEKWAVMKADWERLFEL
                     SGGGKLGAGSLDVARMLAQRWELLRAVSSWMGAPGGASWSGMPGSSWARPPQAHKGFW
                     LEADCELIVYGATEPDAKVTMQGNPITLNPDGTFSFRFALPDGKLEIPIHATNRDGDM
                     SKSVDFTVTRATQRT"
     gene            13210..14361
                     /locus_tag="P5079_02855"
     CDS             13210..14361
                     /locus_tag="P5079_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY28959.1"
                     /translation="MRFPNRVGRWILLPYILILLLFLEIATRLSLVRLWPSDFSRFQD
                     SEIRRYLWTVRQQKPALPLDPAERFDPVRGWALRPGVRNDRTGFHSNSRELRGRREYA
                     DEKPPHVRRVAVFGDSYAAAAKLSDAETYPARLEHLLPNTEVLNFGVMGYGHDQMLLY
                     LRQEGLTYHPDVVILGFVTCDIPRNSTTFPWAAKPRFRLHGQDLLLENFPVPSPDEIV
                     RWEFFHPKLFRLLKTLFSPRDTPGEWRLTTALLEEFCRVARAAGAAPLVVFMPIHDEL
                     SSPDASPAENQLEDHCRSHGIPYFSVRPALAKFPDAKKRFHAGTHWSAEAHALIAGEL
                     SAFLRTLDPTLKEPIPHEQRNAPPKTWERRLADVLNSPRRHTTSKLFLQ"
     gene            14358..15770
                     /locus_tag="P5079_02860"
     CDS             14358..15770
                     /locus_tag="P5079_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRY28960.1"
                     /translation="MTSSPKKNLFLLPLLLAALTAPSRNIPAQGPPQKPNVLLITLDT
                     TRADHLACYGHPTAETPTLDGLAARGVLFEQAITPIPVTDPAHITLFTGLSPDEHGVY
                     ENGIVLEKDPPTLAGLLAANGYDTAAFVSVKHLLTDGYLRGFKTVDGDFSSPERFAED
                     TTDRALRRLEKTGPAPFFLWVHYFDPHAPYFPPERFRRERSPGAVRFETYLALRRQTP
                     GYLSRGITPEEKTAASALLQTHPEADISFFRLTEEDNAVIESWYLGEIAHVDSQLSRI
                     VSLLQQRRLLDDTVVILVGDHGETLRESSHVGQRFRHGSEVYDRQIRVPLIISYPKVL
                     PSGRRIAEQVRLADVLPTLLGLLGIENKSPMDGVDVLASAAPPDLPAYSYSVRQHFEC
                     VRTNEFKLLRDGFNDRMLLFDLKNDPAELTDVKGRRPDVFSDLSEKLARWKQKRGASL
                     RKGDVDPHVLENLKDLGYLR"
     gene            16084..16173
                     /locus_tag="P5079_02865"
     tRNA            16084..16173
                     /locus_tag="P5079_02865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16118..16120,aa:Ser,seq:gga)
     gene            complement(16293..16838)
                     /locus_tag="P5079_02870"
     CDS             complement(16293..16838)
                     /locus_tag="P5079_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28961.1"
                     /translation="MKKNRLGLMWGIFLLGAGPALAEPSESIKYLMSERMSLFDNGLM
                     RIEEHLYRHLGENWGVLVNYDWNKNAIFVRVWRDDIEEAQKTKDSPTPKDMATRAIGE
                     VKKFCGYNEDGKLINPDTGGLIGSYFSHRGYVSKQEPKSIVADVNGIVEITAAVQGKK
                     ERIEGKTKLTEKKVNWSTVKN"
     gene            complement(16860..17423)
                     /locus_tag="P5079_02875"
     CDS             complement(16860..17423)
                     /locus_tag="P5079_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRY28962.1"
                     /translation="MNPVIENIKARRSVRLFDGRKIDKKTLEALIDAARWAPTGMNSQ
                     PWRFVVAQGDEFRKKLSALALPRYKKWLEKMPQELKAMRQEVDEKTGDPAYYGAPVVV
                     FVIGKGMTSDFDCAMACQNIMLAARSLGIGSCWVYIGQLPLDDAEMRGALELKEGEKV
                     YGPLVLGYPKGNFPAPPPKKDPPVKWL"
     gene            complement(17428..17847)
                     /locus_tag="P5079_02880"
     CDS             complement(17428..17847)
                     /locus_tag="P5079_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY28963.1"
                     /translation="MSRFRKIALAVLVVGFSGVLVLAGGTFYMLHAEVKRICVEAGNR
                     FEGDCVESLIATVKAEELPFKTRNEAIWALGQLADAKALPYLTEIQPKTFCQKPCGNK
                     YLCRYELDKAVKWCTQGNLTRWMYAHREDWLEEGRKG"
     gene            complement(17895..17981)
                     /locus_tag="P5079_02885"
     tRNA            complement(17895..17981)
                     /locus_tag="P5079_02885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17945..17947),aa:Ser,seq:tga)
     gene            complement(18219..18800)
                     /locus_tag="P5079_02890"
     CDS             complement(18219..18800)
                     /locus_tag="P5079_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HRY28964.1"
                     /translation="MRARNLLAIGVLICSCACTGQKQSPTTEAPPKEEKKQAATSTEE
                     ATDRLLMALNQGDYAQFSREFDDKMKAALNEAEFAKLRTNILEKIGLYQTRGLPRTLK
                     LKDATVMVYRARFENEPEAEVRVVFREVGGQRRVSGLLFNSPKLRAAAAPAPAAETKT
                     PAASPADAKKETKAEVPPEKKDEAKPGTPKASQ"
     gene            complement(18818..>19172)
                     /locus_tag="P5079_02895"
     CDS             complement(18818..>19172)
                     /locus_tag="P5079_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY28965.1"
                     /translation="FVIAMNDILRSEKAHRTVDSETVGAVPIGVPLITGPAVLTTVLL
                     LTAEHHYVITGPAILLNVLFAGLVFGFAEPIHDFLGKAGTKTISKIAHLLLAAIAVMM
                     VRKGLAQIIATGGFM"
BASE COUNT         3489 a         6359 c         5554 g         3770 t
ORIGIN      
        1 gcgtcgcggt gcgcagcgcc tgcccgaacg ccaccgacaa aacgatcagg ccgatgccgc
       61 agagcacccc tcccaaaata tagccccacc ggaccgccgg ggaggccggg ggcacgaccg
      121 gcgagggcac ggggcgggtc ttcatgggat cagcacagat gccgtccgcc gtccacgcgg
      181 agacattctc ccgtcacaaa atccgagtgg atgaagaaca acacggcctt ggtgatctcc
      241 tccgggccgc cccatcgccg cagcggcgtg gcgcggacga cgtcccggtt ttcgctccgg
      301 gagaggccgg cgggcggcag gatcggcccc ggcgccaccg cgttgacgag gatggcgggg
      361 gcgagctcca gggccaacga ttcggtcagc cccttcaccc cggtcttggc gacgtaatac
      421 ggcaggcggt ccttataccg gggccgcccg ctgaccgcgg tccaatccga aaaattgatg
      481 atgcggccgc cgccggtccg ccgcatgagc ggggccgccc gcagggaaag cagatacgcc
      541 gcccgcaggt cggtgttcag ggcgcggcgc caactcggcc gggcgcggcc ggacggcttg
      601 ccggccagtc cgcccagcgg cactcgctcg tacatcgacg ccatattgac gaggacgtcc
      661 aaacgccgcc acttcttttc caccgacggc accgcccggt ccaggtcctc cgcccgcgcc
      721 aggtccacgc gcagggccat cgcctccacg ccccagttcc ggaccgcccg caccgtggct
      781 tccgcctcgc tccgggagcg ccggtaaagc atggccacgc ggcagccttg ccgcgccaac
      841 gccagggcga tctcccgccc catgcgggtc ccgcccgtca ccagagacac cttcccttcc
      901 aggttcacct tcgccgcctc ccctaaagcg cgtcgcacag cccgcgaaaa aaattgtaaa
      961 ctcctctgcg catgaaaatc tccgaactga tcaaaaccgt gaagccgtcg ttctccttcg
     1021 aattttttcc cccgaagacc gacgacgacg tggcccagct catgcagacc gcacgtgacc
     1081 tgcggacgct tgaccccacc ttcatttcag tcacctgggg ggccggcggc ggcacccagc
     1141 gaaaaaccat agacaccgtg tcggccatca aaacggacct gcgggtggaa agcatggcgc
     1201 atctcacctg cgtcggagct tcttcctggg agatcagcgc ggtgctcgac gaactccaga
     1261 aaaaaggcgt cgaaaacatc ctggcgctcc gcggcgaccc gccgcgcgac cacccctact
     1321 tcgtgccgca ccccgacggg ttccgccacg ccaacgagct ggtggcccac atccgccagg
     1381 gctggaagtt ctgtatcggc gtggccggtt atccggaagg tcatgtggaa tgccccgata
     1441 aagaactgga cctgcggaac ttaaagcaaa aggtggacgc cggcgccgat ttcgtcgtga
     1501 cccagctctt cttcgacaac accgtctact tcaaattcgt cgaacgggcg cgggccatcg
     1561 ggatccaggc ccccatcctc gccgggatca tgccggtgac caacgcccat cagatcaagc
     1621 gcttcaccac cagttgcggg gccaccctgc cgccggccct tctttcccgg ctcgaggctc
     1681 accccgacga caaggcggcg gtggtccaga tcgggatcga ccacgcggcg caacagtgcg
     1741 ccgaactcct ccggggcggg gcccccggga tccatttcta caccatgaac cgctcccact
     1801 ccaccacgga aatcctgcgc cggctgcgcc ggtcttaaaa accgaccccg cttcccatca
     1861 ccaggccgag gcggcggaac gcaacaggct gatgtcgatg cggcggttgc gcgcccggtt
     1921 gtccgtcgac gtgttcggca cgaccggctg atactcgccg taccccaccc cggacaatct
     1981 cttcggatcg agcccttcca cgtccacaaa ataccgtatc accgtgtagg cgcgcgccat
     2041 ggacaactgc cagttggatt cgtattcgtt ccggatcccc accggcacgt tgtcggtgtg
     2101 gccttcgatc atcagcgggt ggggctggtt ccgcatgatc tcggccacgc cgtgcaatat
     2161 cgggaacgcc tccggtttca ggaaagcctt gcccgaggcg aagaggatgg gctcgcgcag
     2221 ggtgatgcgg atgcggtcct cggaaatctc cacgcccgtc atctgctgca acccctggtc
     2281 gacgaaagcc tgccgcacgt ctttggcgat gatctcctcc tggctccgcc gaatggcccg
     2341 gtcgatgcgc gcctggttca cctggccgcc gaaggattcc tggatgatgg agatcccttc
     2401 ctcgaaagcc gtgccgccgg tgagcgaaaa aacgaacagg aggaggaaaa aaatcatcag
     2461 ataggacatg aggtcgccgt aggtcacggc ccacaacgcg ccacggttca gaaggttctc
     2521 gaggtcctcc tcccgctcgc ggaaaatctg catcaggctt ttttctccgg cggggaggat
     2581 tccccgttct gccgtttctg caggtaggcg tggaggcggg tctggatgaa gagcggcatc
     2641 tcattggtga agacgacggc caggatgcct tccacgatga gttccttcgc gatggcctcg
     2701 ttcaggctcc gcatccgcag cttgccggcc accggcacgc agatcaggtt ggcgaagagg
     2761 atcccgtaga acgcggacga caacgccagg gccatggacg gaccgacgct gcggggatcg
     2821 gacatgttct tcaaaaccga cacgatgccg atcaacgtcc ccagaagtcc gaacatcggc
     2881 cccaaaagac ccatggtgcg gaacaggttg cccacctcgc ggtgccgggc gcggatcatg
     2941 ttgatctctt tggccaggat ctcgtaggcg gtcttctgat cgggactgga cagactgacc
     3001 tggatggcac gggagaaaaa accgtcgccg acgtggcgcg cctcgtcctt caacgacatg
     3061 gccccgtcgc ggcgggcctt ctgcgccaga cgcaccaact gagagatgct ctgctccggc
     3121 gaaggggtgg attgcgtctt caagagggac aaccccgcca ggaacgcgtc ccacagttcc
     3181 cgggcagagg agttgatcat ggtggcgaag atggtgccgc ccaacaccaa caccaccccg
     3241 tgtgcgttca ggaaggacgc ccaactctgt ccctggatca tcccgaacac gatgaccgtc
     3301 acgccgccca gcagtccaac gattgtcatc aggtccataa aaattctccg ctcacgccga
     3361 tttcccggcc ggaacgccca accgacccgg caaccggacg aaggacgggg agatctgccc
     3421 ctcacggatc agcttgtaca cggtgcgcag gacacggtcc tgcgcctccc cgatcttttc
     3481 gtcggtcgac gagggcccgc ggtgctgaga ttcctcttcc agcactttcc acgtcagctc
     3541 cgggagcacc gtcttgatgc gcgttttcag ttccgccgat tgtttgagga tggccaccgc
     3601 cagatccgac agctgcacgt tggacaccac ccgcgccagc tcctgctggc tgagcttgtc
     3661 cagatcctcg aagatgaata tccgttcccg aaccgccgtc cccagcgccg ggtccttctg
     3721 cgtgatggac tccaggagct ggcggcgcat gccgggaccg gaggcgttga gcatgtccac
     3781 caccgcctgc accccgccgg tgctgccccg gatgtgcttc tccacggctt ccttgagccg
     3841 ctgcagtttc tccaaatcca ccaccgacgg ccgggacatg gccatcacca cctcgcccgc
     3901 ctgttccggc gagagcaacg ccagaaacgc cgacttcgcc gacggcgtga ggtgctgcaa
     3961 caccagcgcc acggtctcca tctcctcgtt cgacaaaaat tccagcaggt aggccacctt
     4021 gtcggcgggc acctcgaaag tgagttcggg gtagtgcggc agggtccgct cgggcatccc
     4081 cggcgcggaa cggtccgcgg gggaggcgag ctccgccgtc aggaggggct gcttgccgtc
     4141 gggaccggtc aaggccgcgt gggtatccac cggctggagc gtccgcaccg cggaggccag
     4201 ccgatccacc gaacgccgca ccgtggcgag cgccgccagc cccaggacga agaaacacat
     4261 ggccgtcaac atcaccacgg acagggtagc ggctttctgc aatatcggcg acaggagggc
     4321 cgggttctgc agggcctcct cgaacttgga gggcatgcgt atccgcgcga tctcaatcac
     4381 gtccccgcgc gccggatcga tgccgacgaa attcgccacc accgcgcggg cggtgttctc
     4441 cgcgcccgcc gacagccctt tgtccagcag caccttgacg tagactttct tgatggcgat
     4501 cccggccgag aggaaagccg ccgggtccca agcctgggtg ggcggcgcca cggagatcac
     4561 ggtcgccgtg ccctgctcct tctgcacttt ggcggggaac cccggcagcc ccgacgacgc
     4621 cgcggacgtc gccggcgagg tccatttggc cccctccgtc accgcgacgg ttttttgggg
     4681 ctgcgaagct ccgagggcct tgtccaaccg ggacttcatc tcttcggaaa tctccaactg
     4741 cacgttcacc agggcgatgg ccttccgttc ccccatcaac tggtccaacg ccacctggat
     4801 gcgcgtctgc aggtcccggc tgaaacggat ctcgtcctcc acaagggaac ccgccgccgc
     4861 gacggcggcg agggccccca gcagtgaaag gacgacaagg ggtcgtttca ttttttgccc
     4921 cgtttcattt tctcttcggc caattgaacg aaccgagtca attcctcgtc ctccggttcc
     4981 aaggccagcg cctgtctcca gagatcgcgg gcctgctcga aattcccctg ggcgaaatgg
     5041 atggacccca tccgtttata cgccagggcg ttgtccggct ccagcaaaat cacttcccgg
     5101 cagaggatca tcgcttcggt gagccggttc tcgttgaaat tctccgacgc cttttccagc
     5161 ttcaaggaaa ccagttcggg cgtgggccgc acgttgttgc ggtcgagctt gtatccggtc
     5221 tccttctcga tttccccgat gaaccgctgg agccggtcgt ccagcggcca gttgccgagg
     5281 gcgctgccca gacacaggag ggccgtctgg tattcggcgg cgatgtattt gctcaggccc
     5341 tccaaaaaaa catggctctg gttctcctgg cggatgcggt cgtcgaacag ttccagaacg
     5401 cgttccagcc gccggtcgta ggccttgatc tcgaccgaac tcggatcgaa ttgcgccgcc
     5461 tgatggatgc tgcccaccgc ggccgcgaac tcgtaggtat tcacgtatcc gcgcgcgtct
     5521 ttatagaact ccatcgcccg ctccttgtcg atctgcgacg aagaacgggc agacgtcgcc
     5581 atcggcgcgc aacactcgca aagaaccaag aggaatcccc ccagaacgca aagcgccacg
     5641 cccctccgac gagactttaa atcccctccg gacaggaaaa taggcattta tccgtgttaa
     5701 gtatcccctt aaaacggtgc tttgtcaagc cgctttgatt gaaaaaacgc ggcgtttcgg
     5761 ttttcctggt acagaaagaa gggagggctc gatgggagcc ctcccttctt gttacttaat
     5821 ttgttacaag gttctagaag tcgccgccca tcccgccgcc gtgcggcatc gggggcatgt
     5881 tgttcttctt ttccggaatg tccgtgacca gcacttccgt ggtcagcaga agccccgaaa
     5941 tgctggcggc gttctgcagg gccgaacggg tcaccttcac gggatccacc acgcccatct
     6001 tcacgaggtc gccgaactcc cccgactcgg cgttgtagcc gaagttggag tccttgctgt
     6061 tgcggacctt gtccaccgcg atggagccgt ccatcccggc gttctccgcg atggtgcgga
     6121 tgggatcctc caagacgcgg ctgatgatgt tgatgcccgt ctgctcgtcc gggtcagcgc
     6181 tcttgacgcc gtccagcgcc ttctgggcac gaagcaacgg cacgccgccg ccggggatga
     6241 tgccttcttc cacgcccgca cgggtggcgt gcatggcgtc ttccaccttg aacttcttgg
     6301 atttcatctc cgtctcggtg gccgcgccga cttcgatgac cgccacgccg ccgacgagtt
     6361 tcgccaggcg ttcctggagc ttttccttgt cgtagtcgga ggtggattcc tcgatctgct
     6421 tgcggatctg cgcgatgcgg gctttgatgg ccttgtcgtc gccctcgccg cccacgatgg
     6481 tggtgttttc cttctccacc accacgcgtt tgcagcggcc gagcatgtcc acggtggcct
     6541 tttccaattt gaagcccttc tcttcggtga cgacggtggc gccggtgagg atggcgatgt
     6601 cttccaacat ctccttgcgg cggtcgccga accccggggc cttcacggcc gcgcaacgca
     6661 gccgtccctg gagcttgttc accacgagcg tggcgagggc ctcgccctcc acgtcctcgg
     6721 agatgagcac gaacggtttg ccctgctgca cgatcttctc caacagcggc atcaggtcgc
     6781 ccatggcgga gactttcttg tccgtcacga tgatcagcgg gtcttcaaga accgcctcca
     6841 tgcgttccgc gtcggtgacg aagtacgggc tgatgtagcc gcggtcgaac tgcatccctt
     6901 ccaccacttc cagggaggtg tcggcggact tgccttcctc caccgtgatc acgccgtcct
     6961 tgccgacctt gaggatggct tcggcgatga gttcgccgat ctggtggtcg gaggccgaga
     7021 tggtggcgat ctgggtgatc tcctctttcg ccttctcttt ggcgtcgagg ttgaccttct
     7081 tggccacttt cttgagttcg gccaccaccg cttccacggc cttgtcgatg ccgcgcttga
     7141 tgtgcgtggc gttggccccg gcggtgatgt tgcggaaccc ttcgcggagg atggactgcg
     7201 ccaggaccac cgcggtcgtg gtgccgtcgc cggccacgtc gttggtcttg gaagacacct
     7261 cgcggaccag ctgggccccc atgttctcga acgggtcctc cagctcgatc tccttggcga
     7321 tcgtcacgcc gtcgttggtg acggtgggcg agccgaactt cttgtccaac accacgtagc
     7381 gtcccttggg acccagcgtg gctttcacgg cggtggcgag tttatccacc ccggccatga
     7441 tggccttgtg ggcctcatct gaatatttga tctgcttagc cataacgtta cttcctcctt
     7501 taatgtcggt gatcggtgag cggtaaccgg tgaccggcca ccgataaccg gttactgccg
     7561 ttctatttgg attccagaat gcccagaata tcctcttgat gcatgatcag atattctttg
     7621 tcgtcaatct tgatctccgt gccggaatat ttcccgtaca ggatcttgtc cccgaccttc
     7681 acttccatgg ggatcacctt gccgtcgtcc gccaccttgc ctttgccggc ggcgacgatt
     7741 tccccctcct ggggcttttc cttcgcggtg tccgggatga tgatcccgct ccgctcctgc
     7801 ttctccttct ccagcggacg gaccaacacc cggtccccca gaggccgaat cttcacttta
     7861 gagagaactg cgtctaccat ttttagcacc tccttgtatt gactgctaat taaaacttca
     7921 aaaaaatgcc aaccatcacg attggcgttt tcacacggaa aacatcgcct attctaacgc
     7981 gggcaacact tccggtgtgt gtccattata ccgcatctct ccccatggtg tcaagaaaaa
     8041 atgctctgct tcgggacgga acagcgtccc cggatcgcat caaatcgccc gattcatcgg
     8101 gccgctccca tcccgtggag gtttttcaga aacccgccga cctaaagagc cggaaaagaa
     8161 aagggccgga tggggggaat ccctccatcc ggcccctgaa gtcctaccgc gcggtcagct
     8221 tactgacaca ccgcgggttt ggtgccgatg gaccgcgtca ggttcggttg cggcgcagaa
     8281 aacgccccgg cgttggtgaa ccggcagatc tccacaccgg accccgcccc gcccgcatcg
     8341 tccgaattga cggcgaaggt tttggcgaag gttttggtga ccccggctac gcgcaccgtg
     8401 atcacgcagt tggacgcggt tcccagactg caatcgttgt agttgtccac ccgaacgatg
     8461 taggtgccgg ccaccgccgt gcccgtcggc caggtgatgt tctcgttgct gatgccggtg
     8521 ctgggacacc cgacgttgga atccaggtcc aacgtcccct tggtggtcgt cggggctttg
     8581 tccccgtacc agacctccac gttgttgggg tccaccacat gcaggtcgat gtcggccttg
     8641 gtgtcccacg acagactgac ctgaacgtcc ccggtgccca ccgtggccac ctgggtgatg
     8701 ctgatgtcct cgatgggccc gatgttgccg ctggcgtcct gcagtgtcac gaaacaatgg
     8761 aaggagccgg gcagggcctg gccgatgttc aagtccaggg tgagcgtggt ggcatcggcg
     8821 ggaagcgcca acagataata gcccgcgatg gactgcgccg ccgagggggc cgcggccgcc
     8881 ggagccgccg cgagattgga caccgccacg ttacagtaac gaaacgcctg cgctgtcacc
     8941 agcgacaact gggaagaccc gccgttgatg acgccggtgt tgccggcgac ggtcgggttg
     9001 agccccgccg ccgtgccgga ggggatggtt ccgttctgcg gcaccgcgtt ggttccgccg
     9061 ctggaaaccc cggagatgta cgaggtcagt ccggcgagag cggtcgagag tgtcggggtg
     9121 ccggtagcga catcggcgcc gccgaggatc ttggaagaca cgttgttgct cttgcaggcg
     9181 ggcagaacga ggcccatcgc aagaacaaac gcgacgaatt ttttcatgcc gtaacgattc
     9241 ataatcaaat cctccttagc gcttaaatta tttcagctgg aaacgccgcg tggcgcagaa
     9301 cagcagccgc cagtcgccgg cgggacgggt gaggtcgtag ctggaaacct cctcgtcccc
     9361 gatcccgagg gcgagggcgc ccttgaaccg ccattctttc cagtccttgg tgagcccgca
     9421 cgccaagttg atgacgttgc cgtcggagtt gctcaccgac ttgcccgccg acttggtttt
     9481 gccgacgcgc gcgaagtaga gttcgcccgt gaggatcttc ccgcggtagg gcctttccat
     9541 cgccaccgaa gcgaacaaca cctctcccgg gttgaccttg atgcgcgact gcccggcgac
     9601 gtcgaaatcg cttttgaact ccgacttgaa cagatacccg gcgttgaaaa tgccgtgcac
     9661 gtctttggaa agttctcctt ccgccaagta ggacacgccc aggttgaacc cttcgctcag
     9721 gagcttgtcc tggtcgccgg tggggacctc caattgggcc tgcaccgcct tgttttttcg
     9781 gaaggcgtat ttgccggcca ggatggtctg ccccagtccg gaatcggtgt cggaatcggt
     9841 gccgccgctg accttgttgt tgatatacgg caacaccagc ttcgtctccc actgtttttt
     9901 gggggaccag cgcgcctgca ggtccacccg ccggagcgtg ttcttctgct ccacgttggc
     9961 caccttgtat ttatcctgac gctggctggg cgccaggccg acttccacga tcttggtatc
    10021 gatggggacg gcgcgttccg agaagaacgg ttcgccgtgc gccatggccg ccagaggggc
    10081 cacaacaacg aacaacaacc agagccgact ttgccgcatg ggggtgagac ctcctttaag
    10141 gaatgagtgc atcaaagatg acgcgttgcc gttacgattg gtagaccttg tagtctatcc
    10201 aaggaaaaat gttgtcgcgc tcttccaggg aacggacgaa gtcctcccgc agacggttct
    10261 cctgtatctg gtggaagagt tccaggaacc ggctgacgtg ttccctcgtg cgccggtcgg
    10321 agtaggtgcg ggccgtgccg acggtcatca ggaacgccca gtcggacgac tgcgccagaa
    10381 ggagttcccg ggccaactgg ttcaacgccc gcttcagaag gccctcggcg ttggcgtgtt
    10441 tcttggccag atggatcatc ctctccgcgc agatgtgcag gtgcggatag atccagtcgt
    10501 tggaggcgtt caaccacacc tcgtgatagc ccttgtcgcc ccaggaggac ggctccggct
    10561 ccacgatctg ctgctcggga aacttgtcca gatattcctt cggcgtggtg gttttgaaga
    10621 cctgttggtc gtagtggatc tttttgagga ggtattccag gaaggacggc ccctcgaacc
    10681 accagtggcc gaacagttcg gcgtcgtaaa tggaggcgat caccgggggg cggcccatca
    10741 cgcccttgag atactccacc tggcgttcgc ggttgaagag gaagttgccc gcatgggacg
    10801 cggcggtttc ccgggcgtgg ctgggagagt acggctcttt ttccgacaaa gacacacggc
    10861 cggtgacctt gtagtattta atcccgatgt tccgccgcac gccgtcctcg tgcagataag
    10921 gacggatgta gttgtactcc agatcgtagc ccaggtcgcg gtagaactcg cggtagctgt
    10981 agtcgccggg atatccctgc tccgccgacc acacctgatg ggcggtctcc atgtcgcggc
    11041 cgaagaacgc cacgccgccg ggggaataga tcggggcgaa cacgccgaac cgcgggcgcg
    11101 gcacgccgaa caggacgccg tgcgaatcca ggaacgagaa cgagatgccg ttctcgcgta
    11161 aaagcttgtc cacgccgggg gcgtaggcgc attccggaag ccagatgccg cgcggggccc
    11221 ggccgaaatg ccggtggtag tcctgcaccg ccacgcggat ctgcgcccgg cgcgcctcct
    11281 cgtggatcat caacgggagg tagccgtgcg tggcgccgca ggtcaaaatt tccagattcc
    11341 ccgcgtcctg cagggccttg aagcgggtca ataaccgtcc gtgcgaggcc tcgaagatct
    11401 cccgcgcctt gttcaggttg tggtgataca tccgcgccgt ctcctggaac gccgccggca
    11461 ggctgcgcgt gcggtgcagt tccttgtcgg ccagctccag gagcttgtgc agttttttct
    11521 ggtaacgcga cagcaggaga tcgtcggaaa gcatgttgca cagcggcggc gtgagggaca
    11581 tcgtcagccg gtagtgcacg ccttccgcgg ccagccgctc catcatgtcc agcaggggaa
    11641 cgtaggtttc ggtgatggcc tcgtagagcc agtcttcttc gagaaagtcg ggatattccg
    11701 gatggcgcac gaacggcagg tgcgcgtgga gaacgagaca cagatagcct tgcgggtcag
    11761 ccatacaggg tcacctcgat gacgggaaaa acaacggagc cgggccgggc atgtccgcgg
    11821 ccgatgatgc tttacccatg gaacccctca cgcgcgcccc caccggcgga tcaagtccgc
    11881 tgggtggctc gcgtgacggt gaaatccacg ctcttggaca tgtcgccgtc gcggttcgtc
    11941 gcgtggatcg ggatctccag tttcccgtcg gggagggcga aacggaagga aaaggtgccg
    12001 tccgggttca gcgtgatggg gttgccctgc atggtgacct tggcgtccgg ttcggtggct
    12061 ccgtagacga tcagttcgca gtcggcctcc agccagaacc ccttgtgcgc ctggggcggc
    12121 cgcgcccagg aagacccggg catgccggac cacgacgcgc cgcccggcgc gcccatccag
    12181 gaggacacgg cgcggagcag ttcccatcgc tgggccaaca tgcgggccac atccagagag
    12241 ccggcgccga gcttgccgcc gccggagagc tcgaaaagcc tctcccagtc ggccttcatc
    12301 accgcccact tctcgtcgat cacgtcggac acctgcccgg tgggcaggcg gatctcgttg
    12361 gagatcgcca gcagcacaaa gcgcccgtcg ggcaggagca tccccagctc ggcgtgccac
    12421 aggcctccgc gcgccggact gaagatgtac cagttgcgcg cgtccaggtg gatgttgacg
    12481 tcgaacgtct tggcggacgc gccgtcgccg caatgcagcc gcagcacggc ccggccggag
    12541 gtccgcaccg ccgacccgaa atttttctcc gcctcctgcc acgtgtaggg ggccacctcc
    12601 caatacgcgt gcatccactg agggtcccgg gggatgagga caagccgggt aacgccgtaa
    12661 tcccgcggaa gaccaggccc cgtctccacg aaaccctgag agggcggcgt ggaactgggg
    12721 acgttagacg aagacgatga actcatgatt ctcctccact taatataatg agggcaaaaa
    12781 accacacata cggaaaaatt ggttcgtttc catagagagt ctgccggggt acttcgagga
    12841 ctacaccgaa aacgccatcg cggaatgcgg atggcgttgc tgcccattct cgtaggtgcg
    12901 gtatgttaac aaagttcccc gcgcgccgtc aagggaaaaa acaccgcctt ttttgaactt
    12961 tgtccgggtt gatccaattg ccgtgattcc aataaagcgg ggaaacgtac aaaggccccc
    13021 catgacgcca aatactctcc ttccccctct cgacaaaggt ccgaggccct ctccctcggg
    13081 cccccgtccc gtgtaattaa aaattgatac gaattgacaa tgtgatttta tttaattttc
    13141 ggccggagtg gcccgaaaaa tccctaaaaa tgcgcccgct ccactcgatt tttcgatata
    13201 atcgtctcca tgcgtttccc gaaccgggtc ggccggtgga tccttttacc gtacatcctg
    13261 atcctccttc tgttcttgga aatcgccacg cgtctgtccc ttgtgcgtct ctggccgtct
    13321 gatttttccc ggttccaaga ttccgaaatc cggcgatatc tctggaccgt ccgacaacaa
    13381 aaacccgccc tccctctcga ccccgcggaa cgattcgatc cggtccgcgg atgggccctc
    13441 cgcccgggag tccggaatga tcgcacgggt ttccactcga actcccgcga actgcgcggc
    13501 cgccgtgaat acgccgacga aaaaccgccc cacgtccggc gggtggccgt cttcggcgac
    13561 tcctacgcgg ccgcggccaa attatcggac gccgaaacct atcccgcccg gttggaacat
    13621 ctgctgccca acacggaagt cctgaatttc ggcgtgatgg ggtacggaca cgaccagatg
    13681 cttctttatc ttcggcagga ggggctgaca taccaccccg acgtcgtcat cctcgggttc
    13741 gtcacctgcg acatcccccg caacagcacg accttcccct gggccgccaa accgcgattc
    13801 cgactccatg gacaggactt gcttttggag aatttcccgg tcccttcgcc ggatgaaatt
    13861 gtccgttggg aattttttca tccgaaactc tttcgcctcc tcaaaaccct cttctccccc
    13921 cgagacacgc cgggcgagtg gcggctcacc accgcccttc tggaggaatt ctgccgcgtg
    13981 gcccgcgccg ccggcgcggc ccccctcgtt gtgttcatgc ccatccacga cgaactctct
    14041 tcccccgacg ccagccctgc tgaaaaccaa ctggaggacc actgccgttc ccacgggatc
    14101 ccctatttct ccgttcgccc ggccctcgcg aaattcccgg acgccaaaaa acgtttccat
    14161 gccggcacac actggtcggc cgaagcccat gcgctgatcg ccggcgagtt gagcgctttc
    14221 ctccggacgc tcgatccgac gctgaaggag ccgatcccgc acgaacaaag gaacgctccg
    14281 ccaaaaacct gggaacgccg gctggccgac gtcctaaact ccccgcgccg ccacacaacg
    14341 agtaaactgt tcctgcaatg acctcgtctc cgaaaaaaaa cctcttcctc ctgccgctcc
    14401 tcctggcggc cctcacggcg ccctcccgaa acattcccgc ccaggggccg ccgcaaaaac
    14461 cgaacgtcct cctcatcacc ctcgacacca cgcgggcgga ccatctcgcc tgctacggcc
    14521 atcccaccgc cgagaccccc accctcgacg gcctcgcggc ccgaggggtc ctcttcgaac
    14581 aggccatcac acccatcccc gtcacggatc cggcccacat caccctcttc accggacttt
    14641 cccccgacga gcacggggtc tatgaaaacg gcatcgtcct ggaaaaagac ccgcccaccc
    14701 tggccggcct cctcgcggcg aacggctacg acaccgccgc gttcgtgagc gtcaaacatc
    14761 tgctgaccga cggctatctg cgcgggttca aaaccgtcga cggcgatttc tcctccccgg
    14821 aacggttcgc cgaggacacc accgaccgcg cgctccgccg gctggaaaaa accggcccgg
    14881 cccccttttt cctgtgggtc cattacttcg acccccacgc cccctatttt ccgccggaac
    14941 gattccgccg ggaaagaagt cccggggccg tccgctttga aacctatctg gccctccgtc
    15001 gccaaacccc cggctacctg tcccggggaa tcaccccgga ggagaaaacc gccgccagcg
    15061 cccttttgca aacgcacccc gaggcggaca tttccttctt ccgtctcacc gaggaggaca
    15121 acgccgtgat cgaatcctgg tatttggggg agatcgccca cgtcgacagc caattgtccc
    15181 ggatcgtttc cctcctgcaa caacgccgac tcctggacga caccgtggtg atccttgtgg
    15241 gcgaccacgg cgaaacgctc cgggaaagca gccatgtcgg acagcggttt cgacacggct
    15301 ccgaggtcta cgaccggcaa atacgcgtgc ccctcatcat ctcctatccc aaagtcctcc
    15361 cttcgggacg ccgtatcgcc gaacaggtcc ggctggccga cgtcctcccg actcttttgg
    15421 gacttctggg catagaaaac aaatcgccga tggacggggt cgacgtcttg gcctccgccg
    15481 cgccgcccga cctccccgcc tacagctatt cggtccggca acattttgag tgcgtccgca
    15541 ccaacgaatt caaactgctg cgcgacgggt tcaacgatcg aatgctgctg ttcgatctca
    15601 aaaacgaccc ggcggaactc accgacgtca aaggacgccg ccccgatgtc ttctcggacc
    15661 tatcggaaaa actggcgcgg tggaaacaga aacggggggc ctctctgcgc aagggcgacg
    15721 tcgacccgca tgtcctggag aaccttaaag atttgggcta tctgcggtga caccggctgg
    15781 gcggccccaa aaattcccag cagaacacat cctcaatcaa gtataattcc gtttcgtgat
    15841 gggctccaac gcagcgcttt tggggccaac ggcaccgcat cgcggtgacg actccgctgg
    15901 aagggtgcgg gcggtggatt gccggcggat ttctatccgc cgctgaatcg ggccaggcgg
    15961 gcctcctttc ctcggagccc gcggcggcga ggtggcgcag ccgcgaacgc acccaacgat
    16021 ttaacgggcc ccaacgcagc gcttttgggg ccagcggcac cgcatcgcgg tgacgactcc
    16081 gctggaaggg tgcgtgagcg gcttaaacgg cacgactgga aatcgtgtag ggctgaaagg
    16141 tcctcgaggg ttcaaatccc tccccttccg ccatttaaaa acccgcttcg ttcgggctct
    16201 cttttgagcc ggacacgaag cgggtttctt tttgggccgc cgaacagcgg cgaaaacggg
    16261 tcggacgatt ttctatcgtc tcccccggcg tttcagtttt ttacggtgga ccaattcact
    16321 ttcttctcgg tcagtttggt ctttccctcg atgcgttctt tctttccctg aacggccgcg
    16381 gtgatctcca caatgccgtt cacgtccgcc acgatgctct tcggctcctg cttggaaaca
    16441 tacccccgat gactgaaata acttccgatc agccctccgg tatccgggtt gatcagtttc
    16501 ccgtcctcgt tgtatccgca gaactttttg acttcgccga tggccctcgt ggccatgtct
    16561 ttcggcgtgg ggctgtcctt ggtcttctgc gcctcctcga tgtcgtcccg ccacacgcgg
    16621 acgaaaatgg cgtttttgtt ccaatcgtaa ttcacaagga cgccccaatt ttctcccaag
    16681 tgccgataca aatgttcctc gatccgcatg agcccgttgt cgaaaaggct cattcgttcg
    16741 ctcatcaggt atttgatgga ctccgacggc tccgccaagg cgggccccgc ccccagaagg
    16801 aaaattcccc acatcaagcc cagccgattc tttttcatgg acgctccttt accttttatt
    16861 caaagccatt ttaccggcgg gtcttttttg ggcggaggcg ccgggaaatt ccctttcggg
    16921 taacccaaca cgagggggcc gtacactttt tccccctcct tcaattcgag cgctccgcgc
    16981 atctccgcgt cgtccagcgg cagttggccg atgtacaccc aacaactccc gatgcccaaa
    17041 gaccgcgcgg ccagcatgat gttctggcac gccatcgcgc agtcgaaatc gctcgtcatg
    17101 cccttgccga tgacgaagac gacgacgggg gcgccgtaat aggcggggtc gcccgttttt
    17161 tcgtcgacct cctggcgcat ggccttcagt tcctgcggca ttttctcaag ccatttttta
    17221 tagcgcggca gcgccaacgc ggacaacttc ttccgaaact cgtccccctg cgccaccacg
    17281 aaccgccacg gctggctgtt catcccggtc ggcgcccaac gggccgcgtc gatgagcgcc
    17341 tcgagcgtct ttttgtcgat cttcctcccg tcgaacaacc ggacggaccg ccgggccttg
    17401 atgttttcaa tcacggggtt catcgggtca acctttccgt ccttcttcaa gccagtcttc
    17461 ccggtgggca tacatccagc gggtgaggtt tccctgggtg caccatttca cggccttgtc
    17521 caattcgtag cggcacaggt atttattgcc gcacggtttt tgacaaaagg tcttgggctg
    17581 tatttccgtc aaatatggaa gggccttggc gtcggccaac tgtcccaggg cccaaatggc
    17641 ttcgttcctc gttttaaagg gaagctcctc ggccttgacc gtggcgatca acgactccac
    17701 acaatccccc tcgaaccggt tcccggcctc gacgcaaatc cgcttgacct ccgcatggag
    17761 catatagaat gtcccgccgg ccaacaccag aacgcccgaa aatcccacca ccaagaccgc
    17821 cagcgctatt ttcctgaaac gactcatccg tcgattgtcc tgcctttcca ccttaatcgg
    17881 aactcaaata aaaacggagg gggagggatt tgaacccccg gtaggtgtta gcctacactg
    17941 gttttcaaga ccagcgcctt aaaccactcg gccacccctc cagcggagcc gccaccgcga
    18001 tgcggtgccg ctggccccaa gagctctgcg ttggggccca ctaaatcgtt gggtgcgttc
    18061 gcggtggcac cgctccataa aattcagcgg gagtctctct gctccacgcg atcaccggcc
    18121 ccctcaaacg cttcaactcg ctttgcgcgt cggagccgtc tcccctgaaa ttgttgccgc
    18181 gccttttccg acggccgggc cccgggcgac ggaaaccttt attgcgatgc cttgggagtt
    18241 cctggcttgg cctcgtcttt cttttcggga ggcacctcgg ccttcgtctc cttcttcgcg
    18301 tcggccggcg aagccgccgg ggtcttggtt tccgccgcgg gagcgggcgc ggcggccgcc
    18361 ctcagcttgg gcgagttgaa caacaggccg gacacgcgcc gctggccgcc gacctcgcgg
    18421 aaaaccaccc gcacctccgc ctccggctcg ttctcgaaac gcgcccggta gaccatcacc
    18481 gtggcgtctt tgagcttgag cgtccggggc agcccgcggg tctgatacag gccgatcttc
    18541 tccaaaatgt tcgtccgcag cttggcgaac tccgcctcgt tgagggcggc cttcattttg
    18601 tcgtcgaact cccgggagaa ttgagcgtag tccccctgat tgagcgccat gagcagccgg
    18661 tcggtcgcct cttccgtgga agtcgccgcc tgctttttct cttctttggg gggggcttcc
    18721 gtcgtcgggc tctgtttttg gccggtacag gcgcaagaac agatcaggac cccgatggcc
    18781 agcaggtttc gcgcgcgcat gattgtcctc gactttttca catgaacccg ccggtggcga
    18841 tgatctgcgc cagccccttg cgcaccatca tcacggcgat ggcggccaac agcaggtggg
    18901 cgatcttgga gatcgtcttg gtgccggcct tccccagaaa atcgtggatg ggctcggcga
    18961 aaccgaacac cagcccggcg aacaggacgt tcaggaggat ggccgggccc gtgatgacgt
    19021 agtgatgttc cgccgtcaac aggaggaccg tggtgagcac cgccgggccg gtgatgagcg
    19081 gcacgccgat gggcacggcg ccgacggtct cggagtccac cgtgcggtgc gctttctcgc
    19141 tgcggaggat gtcgttcatg gcgatcacaa ag
//
LOCUS       DAOTFD010000025        19104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1905_length_19104_cov_4.247520, whole genome shotgun sequence.
ACCESSION   DAOTFD010000025 DAOTFD010000000
VERSION     DAOTFD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_19104_cov_4.247520"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5079_02900"
     CDS             <1..462
                     /locus_tag="P5079_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY28966.1"
                     /translation="ALADTAARVRETAVALLRESRRDLTESVREWRQSAALLIQRHRA
                     FLSESSRGLRLNVRRHMDMGRERLLQIRAQLGRHAVEKIRQERERLAAFAKECRLKDP
                     RRLLERGYSLITQNGRLVKRMDDVGEGQQVEAQVGDGFFSATVLRKRKVTS"
     gene            459..707
                     /gene="xseB"
                     /locus_tag="P5079_02905"
     CDS             459..707
                     /gene="xseB"
                     /locus_tag="P5079_02905"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRY28967.1"
                     /translation="MKKTDKSPGYGESYEELQKILAALEHGEIDVHDLSEKVQRAAEL
                     IEFCQSRLKSAEVEIKRVVEKFQEKTSADDEDGEKTSS"
     gene            738..1196
                     /locus_tag="P5079_02910"
     CDS             738..1196
                     /locus_tag="P5079_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRY28968.1"
                     /translation="MILATLCYVKRGGKTLMLHRVKKLNDIHQGKWNGLGGKMEPGET
                     PEECVIREVREESGLRIREPVLKGFLTFPGFDGVDDWYVFVFEARRFSGRLIDSPEGN
                     LRWVADAAVSRLDLWEGDRIFLRWMRGKRFFSGKFTYKDERLRRFGACFY"
     gene            1295..1813
                     /gene="def"
                     /locus_tag="P5079_02915"
     CDS             1295..1813
                     /gene="def"
                     /locus_tag="P5079_02915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY28969.1"
                     /translation="MKEPHLLPIRKYGDPVLRRKTKPVAVFDDELRKTIQAMFRTMYA
                     EPGIGLAAPQVGLSLRLAVVDVQSDGKNRPLVLINPKIEEARGGVYGMEGCLSLPGLM
                     FNIRRAEWVRVSAINEKGMPVSLSGDGLFARCLQHELDHLDGKLVIDRVPLHRRLKAL
                     WEIRKRKKAGLW"
     gene            1827..2771
                     /gene="fmt"
                     /locus_tag="P5079_02920"
     CDS             1827..2771
                     /gene="fmt"
                     /locus_tag="P5079_02920"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY28970.1"
                     /translation="MLNTIFLGTPDVAVPFLKVLAEKSRLLGVVTMPDQPVGRGYELA
                     CPPVKNAALGLGVPVLQPAALRAPAVEEALRRWGAADLGVAVAYGKIIPLNIFSYPRL
                     GMLNVHFSLLPKYRGAAPVQWALIRGERRTGVSLFQIEEALDSGPIYLQAGEDIRTED
                     NSLTLRARLVEIGVRLLEDALSLFERGECFPKKQEGEPSLAPSLRKVDGLIHWNLRSA
                     EDVINLIRGTYEWPSAYTFFRGQMLKIREAGVARGNGGEPGEIVGLEKGQGFLVRCAK
                     EALLVRRVQPEGKKEMGAMDFWNGARLKLGDRFESPAP"
     gene            2801..3664
                     /gene="htpX"
                     /locus_tag="P5079_02925"
     CDS             2801..3664
                     /gene="htpX"
                     /locus_tag="P5079_02925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRY28971.1"
                     /translation="MNYVKTFVLLLALTILFLVVGQVLGGQQGMAVAFIMAVVMNFVS
                     YWFSHKIILMIYRARPAEESDYPQLHRIVRNLTQVAGLPMPKIYIIPTQTPNAFATGR
                     NPENAVVACTEGILRLLNEKELAGVIGHELGHVKNRDILISSIAATVAGAIMMLANMA
                     RWAAMFGGRGDRDRENSNPYVGLAALLATAILAPLAAMLIQAAISRSREYAADASGAK
                     TCGDPLALASALRKLEAGVTVHPLSQSPTATAHLFIVHPFSGKSMLNIFSTHPPTEDR
                     IDRLEKMVGRI"
     gene            3729..5102
                     /gene="rsmB"
                     /locus_tag="P5079_02930"
     CDS             3729..5102
                     /gene="rsmB"
                     /locus_tag="P5079_02930"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRY28972.1"
                     /translation="MKIVGASAAEKDGASARSAALAVLNRWGRGGGDLRTILADVLRK
                     GNIPPQDRAFCRDLAAGTICRLNSLDWILDSFLNPPQRPLQPIVRWILRLGTFQLVFM
                     TDRVPAYAAVNESVKLAGKAAAGFVNGVLRAVERGRGSLPWPDEQTDRVLSLSLKYSH
                     PEWFVRRWLDRLGLEECRSFLEKNNQSPPMTVRVNTLRVTRDDLQKQLEQQGVAATPS
                     SLTRDALILETRPSLLETEAFHKGYFIVQDEASQIVADLLEPEPGETILDVCAAPGGK
                     TTHLAQRMENRGKVLAVDVSRERLRLVEENCVRLGVGIAVLLCGDARKLSFRDAAFDR
                     VLLDAACSGLGVLRRWPDVRWNKKPEDIAAQLAPTQKELLKNAARCVKPGGTVVYSVC
                     TLELEETEAVMGHFLGENPDFEVDHARSFLPSVLAGAVSPEGYVRLFPHRNGTDGFFI
                     ARLRRKG"
     gene            5103..6191
                     /gene="rlmN"
                     /locus_tag="P5079_02935"
     CDS             5103..6191
                     /gene="rlmN"
                     /locus_tag="P5079_02935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRY28973.1"
                     /translation="MMSHQRVSGTHSQDIPRRESLLEWPFHEWPALLGLSASEKYRVR
                     QLMEWIFHRRVADFGQMSTLPQGLRQSLGEKFTLRVLEFASREISKRDGAQRLNFRAA
                     DGKSIPAVCLSGKEEGGERHSLCLSTQVGCAWGCAFCASGRLKFQRNLTVSEILGQVF
                     GAEEATGKKVDSLLFMGMGEPLANYSNLRLALHALRSPMGFNYGARHVTVSTSGLVPQ
                     MLKLCTEAPKMNLAVSLHAADDETRRRLMPKAAEWPIKEVLKAAWAYQKGAGGGRVTF
                     EYVLLRGINDSLRAAQRLSNLLRGRKAWVNLIPYNPVPGLPYEKPEEVAVRRFEEVLV
                     TRGIFVRLRRPQGDDISSGCGQLGNPAR"
     gene            6273..7277
                     /locus_tag="P5079_02940"
     CDS             6273..7277
                     /locus_tag="P5079_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRY28974.1"
                     /translation="MKKFFSKEELSGRLDRLIAVMRRHPAIAALLIAAFILLVAFGAF
                     LAVVEGVIHARREIIVPDLQGKTLEQALDAIASADLSLAKAAVEFDESFPPGTIIKQV
                     PVPGLKVREGKVLRVTLSSGGQVVFAPDLTNRPLPDAQNALRSVGLIVGAVTEAYSQL
                     HESGWVMEQTPLSGAVLNRGQMVDLRISKGPPPEGMDLMPEFVNQPLSRAMEWIHGKA
                     WKAEVREVVKPEILPGMVISQTPKPDDLVNGMTPIAFEVSQSTTAPTNTQTVRYEVPA
                     GSDRVLVRMVIRDERGEREVFSGYQNAGALVEVPVVIRGASRVRIFVNGVLIEERLLE
                     "
     gene            7289..7954
                     /gene="rpe"
                     /locus_tag="P5079_02945"
     CDS             7289..7954
                     /gene="rpe"
                     /locus_tag="P5079_02945"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRY28975.1"
                     /translation="MPRIAPSILSADFACLGKEVERMERAGADWLHVDVMDGHFVPNL
                     TVGPVVVRWLKKRTRLDMDVHLMIEAPEKFIPAFAEAGAWNITVHGEACADPRRTLRL
                     IKKCGCRAGLSLRPRTPIQKIAPYLGAVDTVLVMTVEPGFGGQRFMPEMLPKVSWLRE
                     YQRRSGKKFWIEVDGGINTQTAPRVVQAGATVLVAGTAIFGQPDPKRALHQLRRAASS
                     PVL"
     gene            complement(7974..8966)
                     /locus_tag="P5079_02950"
     CDS             complement(7974..8966)
                     /locus_tag="P5079_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY28976.1"
                     /translation="MATLISLPARTAPKRLLLCGLLLFGAIAFLLGAEKKPQFNLILV
                     GWDAVGRDDIKDALAKNQLPHLRRLIEKGSLVSIDVLHFSDTTAGWAEIFTGYGYEIT
                     GAFNNSEYREIPKGYTLFERLQERFGRENIATGIMAILPTDWTFTDDDPTDERPPLWN
                     PANPDSIINKGLISFSARSADEFDSSHFRTNPKTMAFLEKYRNMPFFLFVHTKMVDHF
                     GHVNGEGSPEHRDALVRIDKHLGKIMAKLKELHLEKKTLIYVTSDHGFDKKRQNHISA
                     PRVFLASNDPKIIRGGEQADIAPTILDRYGFDLSKIEPPLDGRSLLRRYKKPKW"
     gene            complement(8950..9309)
                     /locus_tag="P5079_02955"
     CDS             complement(8950..9309)
                     /locus_tag="P5079_02955"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY28977.1"
                     /translation="MSLDKGHAAETQAKSYLESKGYVFLAANYRTRYGEVDLVMQEGD
                     ALVFVEVKSRKTRAFGAPEEFVTAPKQGKIVKAALEYVKEHRRVKSPLRFDVVAIENG
                     RVLHYENAFESSLGYTH"
     gene            complement(9309..9983)
                     /locus_tag="P5079_02960"
                     /pseudo
     CDS             complement(9309..9983)
                     /locus_tag="P5079_02960"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            complement(10058..10393)
                     /gene="rplS"
                     /locus_tag="P5079_02965"
                     /pseudo
     CDS             complement(10058..10393)
                     /gene="rplS"
                     /locus_tag="P5079_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(10431..11138)
                     /gene="trmD"
                     /locus_tag="P5079_02970"
     CDS             complement(10431..11138)
                     /gene="trmD"
                     /locus_tag="P5079_02970"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY28978.1"
                     /translation="MKPKSIPLRIDVLTIFPRMFPGVFSESLLGKAQHKGLVRIAVHD
                     IRDHTEDRHRSVDDRPYGGGPGMVLKPEPVFRALKATGAMKKRGKKPFVVYLSPQGKP
                     LTQKLAGELRLKKHLIVLCGHYEGIDERLLAWVDMEVSVGEAVYTGGEIPAMALVDAV
                     TRTLPGVVKEADSLFWDSFADGWRGRLDCPHYTRPAAWRGRTVPSVLLSGDHKAIQRW
                     RQEQSLKNTRKKRPDLL"
     gene            complement(11128..11376)
                     /locus_tag="P5079_02975"
     CDS             complement(11128..11376)
                     /locus_tag="P5079_02975"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRY28979.1"
                     /translation="MTVPDRSYRVSDLDGCHVVTESGEVLGTLKDVYPTGANDVFVVV
                     GAREYLIPAMKETVLEIDVSAKRILVRLPPGLREIYET"
     gene            complement(11373..11612)
                     /locus_tag="P5079_02980"
     CDS             complement(11373..11612)
                     /locus_tag="P5079_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRY28980.1"
                     /translation="MASPLEFVLSIVQCLVDSPEKVVGRWVESPEGGRVDVRVAPEDR
                     GKVIGKGGRNIQSLRRLVSAAFAKDNQRIGVELAE"
     gene            complement(11633..11893)
                     /gene="rpsP"
                     /locus_tag="P5079_02985"
     CDS             complement(11633..11893)
                     /gene="rpsP"
                     /locus_tag="P5079_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY28981.1"
                     /translation="MVRIRMQRVGRPHKPHFRIVAIHGTKARDAASLEVIGHYHPEEK
                     TEKIVVDTERLKYWISKGAQPSDTLRTILVTKGIWGQIQTAS"
     gene            12255..13775
                     /locus_tag="P5079_02990"
     CDS             12255..13775
                     /locus_tag="P5079_02990"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRY28982.1"
                     /translation="MNAFAKPFSTSVAAARTCYSSEVVTPQDVEKDERSRERRHALAD
                     SIYRAGHHTTLQHAEFQFVLENVSRLFIWSFLHSHPFYNSEQVSQRYVPVEPGRLAVP
                     PLPEKPRALFQEIAAQLMTAYERLTELVYPSAAGEYERIFPLRNVREPGWGKMVRRRA
                     QEAARYVLPLATHAHLYHTVSGLTLHRYHRLCLAYDAPLEQRTVVEKMLAEVRALDPL
                     FVDHMEDPLSLEETPEYELFKQFHENRPPSREFLDEFDRSLGGRISKLVDYKVNGEAV
                     LAQAVRSVLGVAQRRLADETAIDAVLDPSRNRYLGGSLNLASHSKLMRALLHMNFTFR
                     RKLSHAADSQDQRHRMTPASRPLLAGHQAVEPDYIVPSLVKETPAALELYGETFEKLW
                     KNLRRLSEMGVKQEFVLYLLPNAVPVRYEESGDLLHYHHKWSKRLCYLAQEEIWRACL
                     DEVAQVKEVAPRVARHLGPPCALRSAAGQRPFCPEGDRFCGVKVWQLPLESYRRLI"
     gene            13860..15149
                     /locus_tag="P5079_02995"
     CDS             13860..15149
                     /locus_tag="P5079_02995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRY28983.1"
                     /translation="MATLIVVGAQWGDEGKGKIVHLLGKKADYIVRYQGGNNAGHTVV
                     FDGKTFVLHQIPSGILQPGKKCIIANGVVLDPAALKEEILFLESRGIRVRGRLAVSAW
                     AHLILPYHKYLDAHREAGSDKIGTTKKGIGPAYSDKVGRTGIRLADFMEPDLFRELVE
                     RNLQEKAPVLEQVISLDALRRETFKDYDALRKFFEPFVANTPALLQEALAAKKNVLFE
                     GAQGALLDVDFGTYPFVTSSNPVAGAACVGAGVGPGAIDEVLGVVKAYTTRVGDGPFP
                     TELTGEMGEFLRSRGQEFGATTGRPRRCGWFDAVVMRHSVRVNGITRLALTKLDILEG
                     VDPIRVCVAYRYKGKLLKDFPASRQAQAAVAPVYKDLPGFTGPVKGITQLNKLPVAAR
                     DYVRFLEKQSGAPVGFISMGRSRDETILIDKRFRWTP"
     gene            15146..16267
                     /gene="carA"
                     /locus_tag="P5079_03000"
     CDS             15146..16267
                     /gene="carA"
                     /locus_tag="P5079_03000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRY28984.1"
                     /translation="MNAVLALETGRVFVGRSFGAVGETSGEVVFCTALAGYQEILTDP
                     SYQGQIVMMTYPHIGNYGTFDALNESRKPFAAGFIAREFSDVASHSRSTASLAEYMKK
                     HGVVGIDGLDTRALTRHLRQLGVCRGIISTGNVSPKKLIERAKKTAPMAGRDLVSEVS
                     CSSAYEWRETGEELEVVPPSAGRRKVVVMDFGVKRNTLDSLVRRGCDVTVVPAQTTAQ
                     EILTLQPSGVLFSNGPGDPAAVTYAIETVKRLIGACEKGQVDALFGICLGHQLLGLAL
                     GGKTFKLKFGHRGANHPVKELSTGKVEVTTQNHGFCVDVDSLAAKEVELTHINLNDRT
                     LEGFRHKKLPIFAVQYHPESSPGPHDSRYLFDRFVSLMK"
     gene            16307..16975
                     /locus_tag="P5079_03005"
     CDS             16307..16975
                     /locus_tag="P5079_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY28985.1"
                     /translation="MHTVLLIACKNEERTIGALLPEAKKYVKDIVVLDGRSSDSSAKI
                     ARESGVEVVADAGRGKGAALRRAFEERDGDVFVTMDADGSHDPKDIPALMRPILEGQA
                     DLVIGSRLRGGSEEFVGDLEKTIRVVGSLIITYAINKRFGAQITDSQNGFRAIRAETA
                     RKLKLNSNSTTIEQEMLAKTLKIGGRVVEVPSHENARSFGRSHIRLLRDWPKYLWSLM
                     IDLV"
     gene            17017..18444
                     /locus_tag="P5079_03010"
     CDS             17017..18444
                     /locus_tag="P5079_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY28986.1"
                     /translation="MENKGRPRTILLLNPPAPEVMVREGRCMQRRGAWTTVWPPLTLA
                     TLASIFLQQGHVVRLRDCPVEGVGWEGLESLLREFVPDFVVINTATPTIVNDLRAAAL
                     AKGVCPAVKTIAIGIHPSALPGATLKLCPDLDAVCIGEPERAVPEWVGGKVGPAGAGV
                     LSQGDRDTGVRSTAKYLENLDELPFPAWQLVDRRNYRLPFSAREFLLINVGRGCPQGC
                     LFCAARTYYGNAVRLRSVDRVIREMKRNAADFAVSDYLFWAESFTQERDYAASLCEAI
                     LKERLDVRWVCNARVDQVDGDLLRLMKRAGCWMIGYGVESLAEEVLRRNRKNVVPRQI
                     EEAVVTAKAAGLAVMAHCVFGLPGETEETLKVTESGIRRLPVDFVQYYTAVPFPGSEL
                     YAWAVREGRLLSDDFVRFEQNSTVLRLDGLPAEKIAGFRRTAYLRFYLRPRVIWNVFR
                     LVLSRGGPADFLRTFFDFGRWAVSR"
     gene            complement(18400..>19104)
                     /locus_tag="P5079_03015"
     CDS             complement(18400..>19104)
                     /locus_tag="P5079_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28987.1"
                     /translation="VILRLGTPKYHPWPGVMLFIPLLFRFFSSNSPLKRSAQITAFLY
                     GMLVVGYFSWALRPKLFFVPHDPPRDIIRLGHSLKSLWDNRFLNEEETVLVELWKGGP
                     PSEENGKITLSALTPFKSPDHILFDRENPDSFDLVNGEYVRTRPDERCLLDLPEEEFK
                     SYLQMKKVRLVLAHSKTAETLLRRILPVSFPAGPVTCHVADASTAEKFRPLFQRPANP
                     TDLAGNSPTAEVEKSP"
BASE COUNT         3627 a         5295 c         6222 g         3960 t
ORIGIN      
        1 gcgctggccg acaccgccgc ccgcgttcgg gagacggcgg tcgcgctcct gcgcgagagc
       61 cgccgcgatc tgacggagag cgtccgggag tggcgtcaat ccgccgcgct gctcatccag
      121 cggcaccgcg ctttcctctc ggaatcttcc cgcggcctgc ggctgaacgt ccgtcgtcat
      181 atggacatgg gccgggaacg gcttcttcaa atccgggccc agctcggacg acacgcggtg
      241 gaaaaaatcc gacaagagcg ggagcgtctg gcggcttttg cgaaggaatg ccggttgaag
      301 gacccccgcc gcctgttgga gaggggatac agtctcatca ctcagaatgg ccgtttggtg
      361 aagaggatgg acgatgtggg ggaggggcaa caggtggagg cccaggtggg ggacggtttt
      421 ttcagcgcga cggttttaag aaaaaggaag gtgacgtcat gaagaaaacg gataagtcgc
      481 cgggatacgg cgaatcatac gaggaattgc aaaaaatcct ggccgcgctg gagcacggcg
      541 agatcgacgt gcacgacctc tccgagaaag tccagcgcgc cgccgaactc atcgagttct
      601 gccagtcacg cctcaaaagc gcggaagtcg agatcaagcg ggtggtggaa aaatttcagg
      661 aaaagacgtc ggcggacgac gaggatgggg aaaaaacgtc ctcctaagcc ggccggcggc
      721 ttttgtgtga cggcggtatg atactggcca cgctgtgcta cgtaaaaagg ggcggaaaga
      781 cgctgatgct ccatcgcgtc aaaaaactca acgacatcca ccaggggaaa tggaacggtt
      841 tgggcggcaa gatggagccg ggggagaccc ccgaggaatg cgtgatccgc gaagtccggg
      901 aagagtccgg gctgagaatt agggaacccg tgttgaaagg cttcctgact ttccctgggt
      961 tcgacggtgt ggacgattgg tatgtgttcg ttttcgaggc gcgccggttc agcggacgtc
     1021 tgatcgactc gcccgagggg aatttacgct gggtcgcgga cgccgcggtg tcccggttgg
     1081 acctctggga gggggaccgg atttttttgc gctggatgcg cggaaaaagg tttttttccg
     1141 ggaagttcac ctataaggat gaaaggctcc ggcgtttcgg cgcctgcttt tattgaccct
     1201 tgggggaagg ggtaaagaat actaaatagt catgtattga gggttccctc tttgttataa
     1261 tttagaaagg gacggatgtc cggaaagtca tgccatgaaa gagccgcact tgttgcccat
     1321 acggaaatac ggggacccgg tcttgcgccg caagaccaag ccggtcgccg tgttcgacga
     1381 cgaactgcgc aagaccatcc aggccatgtt ccgcaccatg tacgcagaac ccgggatcgg
     1441 cttggcggcg ccgcaggtgg gcctgtccct ccgattggcg gtggtggacg tccagtccga
     1501 cggaaagaac cggccgctgg tgctcatcaa tcccaagatc gaagaggccc ggggcggggt
     1561 ctacggcatg gaagggtgtc tttccctccc gggtctcatg ttcaacatcc gacgggcgga
     1621 gtgggtccgc gtttccgcca ttaacgaaaa aggcatgccg gtgagcctgt ccggcgacgg
     1681 tctcttcgcc cgctgccttc agcacgaatt ggatcacctg gacggcaagc tggtcattga
     1741 ccgcgtgccg ctgcatcgga gactgaaggc cttgtgggaa attcgcaaac ggaaaaaagc
     1801 gggactctgg tagggccgaa actttcttgc tgaacaccat ttttttggga actcccgacg
     1861 ttgctgttcc tttcctgaag gtcctggcgg aaaaatcccg tctcctcggc gtcgtcacca
     1921 tgcccgatca accggtcggg cgcggttatg agcttgcctg cccgccggtc aaaaacgcgg
     1981 cgcttgggct gggcgttccg gtccttcagc cggccgccct ccgggccccc gccgtggaag
     2041 aggcgttgcg tcgttggggg gcggcggatc tcggcgttgc ggtggcttac gggaagatca
     2101 tcccgctcaa tattttctcc tatccgcgct tggggatgtt gaacgtccac ttttccctgt
     2161 tgccgaaata tcgcggggcg gcgcccgtgc agtgggccct catccgggga gagcgccgta
     2221 cgggcgtgag cctctttcaa atcgaagagg ccttggattc cgggcccatc tacctccagg
     2281 cgggggagga tattcggacc gaagacaaca gcctcacatt gcgtgcccgg ttggtggaaa
     2341 ttggcgtaag attgttggag gacgcgctct ctctgttcga acgaggcgaa tgctttccaa
     2401 aaaaacagga gggcgaaccc tccctggccc cgagtctccg taaagtggac ggactgatcc
     2461 attggaacct ccgatcggcg gaggatgtta taaacctgat acgggggacg tacgagtggc
     2521 cgtcggccta cacgttcttc cgcggacaga tgctcaagat cagggaggcc ggcgtcgccc
     2581 gtgggaacgg aggggaaccg ggagagatcg tcggcctcga aaaaggacaa ggttttctgg
     2641 ttcgatgcgc gaaagaggcg ctcttggtgc ggcgggttca gcccgagggg aaaaaggaga
     2701 tgggcgcgat ggatttttgg aatggagcga gattgaagct gggcgaccgt tttgagtcgc
     2761 ctgcgccgta gggaaaatag agagattcgg aggtccggat atgaattatg ttaaaacgtt
     2821 cgtgttgttg ctggcgctga ccatcctgtt cctggtggtc ggccaggtcc tcggcggcca
     2881 gcaggggatg gcggtcgctt tcataatggc ggtcgtgatg aacttcgtct cctactggtt
     2941 ttcccataag atcattctga tgatctatcg ggcgcgaccg gccgaagaga gcgactatcc
     3001 gcagctgcac cgcatcgtgc ggaacctgac ccaggtggcc ggcctgccca tgccgaagat
     3061 ttacatcatc cccacgcaga cgccgaacgc gttcgccacg gggcgcaatc cggaaaacgc
     3121 ggtggtggcc tgcaccgagg gcatcctgag actcctgaac gaaaaggagt tggcgggggt
     3181 catcggacac gaactcgggc acgtgaagaa ccgggacatc ctcatttcca gtatcgcggc
     3241 gaccgtggcc ggcgccatca tgatgctggc caatatggcc cgctgggccg ccatgttcgg
     3301 cgggaggggg gatcgcgacc gggaaaattc caatccctat gtcggtttgg cggcgttgct
     3361 ggcgacggct atcctggctc ccctcgcggc catgctgata caggccgcca tttcccgatc
     3421 ccgtgaatac gccgccgacg ccagcggggc caaaacgtgc ggcgatccgc tggccttggc
     3481 cagcgcactg agaaaactcg aggccggcgt gactgtccat ccgctgtcgc aatcgccgac
     3541 cgccaccgcg catttgttca tcgtgcaccc tttcagcgga aagagcatgc tgaatatttt
     3601 ttccacccat ccgcccacag aagaccggat tgatcggctg gaaaaaatgg tcggacgcat
     3661 ctgacccctc ggcgtcttca cggacatcgt tctctccggg gccccgtgtc ggctgacttt
     3721 ttccgtcggt gaaaatcgtg ggggcctccg cggccgaaaa agacggcgct tccgcccgtt
     3781 ccgccgccct ggcggttttg aatcgctggg ggagaggggg gggcgacctg cggacgattc
     3841 tggccgatgt tctgcggaag gggaacatcc ctcctcagga ccgggctttc tgtcgcgatc
     3901 tggccgccgg gacgatctgc cgcttgaaca gcttggattg gattttagac agcttcctca
     3961 accctcccca aaggccgctt cagcccattg tccgttggat tttgaggctc gggacatttc
     4021 agctcgtttt catgacggac cgcgttccgg cttacgcggc ggtgaacgaa tcggtgaaac
     4081 tggccggaaa ggccgccgcc ggtttcgtca acggcgtttt gcgcgccgta gagcgtgggc
     4141 gaggttcgct gccttggccc gacgaacaga ccgaccgggt gctctcgctg tccctgaaat
     4201 attcccatcc ggaatggttc gtccggcgtt ggctcgatcg tttggggttg gaggagtgca
     4261 ggtcttttct tgagaaaaac aatcagtctc cgccgatgac ggtgcgcgtc aatacgttgc
     4321 gtgtgacgcg ggacgatctt caaaaacagt tggaacagca aggagtggcg gcaacgccgt
     4381 cgtctttgac gcgggacgct ttgatccttg aaacacgccc ctcccttttg gaaaccgagg
     4441 cgttccataa aggatatttc atcgttcagg acgaggcctc tcagatcgtg gccgacctgt
     4501 tggagcccga gccgggggaa acgatcctgg atgtttgcgc cgcccccggc ggaaaaacga
     4561 cccatttggc ccagcggatg gaaaatcggg ggaaggtcct ggcggtggac gtttctcggg
     4621 aacgattgag attggtcgaa gaaaactgcg ttcggttggg ggtgggaatc gccgttttgc
     4681 tttgcggtga cgcccggaaa ctctctttcc gggacgcggc gttcgatcgg gtgctcctgg
     4741 acgcggcctg ttccgggctc ggtgttttac gtcgatggcc ggacgtgcgc tggaacaaga
     4801 agccggagga tatcgccgcg cagctggcgc cgacgcagaa ggaactgttg aaaaacgccg
     4861 ctcgttgcgt gaagcccggc gggacggtgg tctattccgt gtgcactttg gaattggagg
     4921 agacggaggc ggtgatggga cattttttgg gggaaaaccc cgatttcgag gtggatcacg
     4981 cgcggtcttt tttgccgtcc gtcctcgccg gcgccgtttc gccggaagga tatgtgcggc
     5041 ttttcccgca tcggaacggg accgacggtt ttttcatcgc ccgccttcgg cggaaaggct
     5101 gaatgatgtc ccatcaaaga gtttcgggga cgcattcgca ggatatcccc cggcgtgaat
     5161 ccctgctgga gtggccgttc cacgagtggc cggcgcttct gggtctgtcc gcctcggaga
     5221 aataccgggt ccggcagttg atggaatgga tattccaccg ccgcgtggcc gatttcggac
     5281 agatgagcac gctcccccag gggcttcggc agtctttggg cgaaaaattt actttgcgtg
     5341 ttttggaatt cgcctcccgc gaaatatcga aacgcgacgg ggctcagcga cttaatttcc
     5401 gcgccgcgga cgggaagtcc atcccggcgg tgtgtctgtc ggggaaagag gagggcgggg
     5461 agcggcattc cctgtgcctc tccacgcagg tcggttgcgc gtggggatgc gcgttttgcg
     5521 cctcgggacg gctcaaattt caacggaatc tgaccgtttc cgaaatattg gggcaggttt
     5581 tcggagcgga ggaggcgacg ggaaaaaaag tggacagtct cctgtttatg ggcatggggg
     5641 aaccgctcgc caactattcg aacctgcgtc tggcgttgca cgcgctgcgt tctccgatgg
     5701 gtttcaatta cggggcccgg catgtcacgg tgtccacctc cgggctcgtt ccgcagatgt
     5761 tgaaattgtg cacagaggcc cccaagatga atctggcggt aagcctccat gccgccgatg
     5821 acgagacgcg ccggcggctt atgcccaagg cagccgaatg gcccataaag gaagtgctga
     5881 aggccgcgtg ggcgtatcaa aagggggcgg gcggcggccg ggtgacgttc gaatatgtcc
     5941 ttttgagggg catcaatgat tccctccgcg ccgcccagcg gctgtctaat ttgttgcggg
     6001 ggcggaaggc ctgggtgaac ctgatcccgt ataatccggt gcccgggctt ccgtatgaaa
     6061 aacccgagga ggtggccgtc cggcggttcg aggaggtcct cgtgacgcgc ggtattttcg
     6121 tccggctgcg ccggccccag ggggacgaca tctcttccgg ttgcggccag ctggggaacc
     6181 ccgcccgctg agacggtcga gcttgagata tgttatgatg attttcaaaa tcgtcgggag
     6241 tttttgagga gcatatgttt ttttccgaaa gaatgaagaa atttttctca aaagaggagt
     6301 tgtccggtcg gctcgaccgg ctgatcgccg tcatgcggcg gcatcccgcc atcgcggcgt
     6361 tgttgatcgc cgcgttcatt ctgctggtgg ctttcggcgc ttttttggcg gtggtggaag
     6421 gggtcatcca tgcgcggcgg gagatcatcg tgccggacct gcaggggaag accctcgaac
     6481 aggcgctgga cgccatcgct tcggcggatc tgtcgctggc caaagccgcg gtggaattcg
     6541 atgagtcctt tcctccgggg accatcataa aacaggtccc ggtgcccggg ctgaaagtga
     6601 gggaggggaa agtcctccgt gtgacgctct ccagcggcgg tcaggtggtg ttcgctccgg
     6661 atctgaccaa tcggcccctg ccggacgccc aaaatgccct gcgttcggtc gggctcatcg
     6721 tcggcgccgt gacggaggcc tattcgcaac ttcatgagtc cggttgggtg atggaacaga
     6781 cgccgctttc cggcgcggtg ttgaaccggg gacagatggt ggacctgcgg atttcgaagg
     6841 gaccgcctcc ggaaggcatg gatttgatgc cggaattcgt caatcagccg ttgtcccggg
     6901 cgatggagtg gatccatggg aaagcgtgga aggcggaggt tcgggaagtg gtcaagccgg
     6961 agatcctgcc gggcatggtc atcagccaga cgccgaaacc cgacgatttg gtgaacggca
     7021 tgacgcccat cgcttttgag gtgagccagt ccacgaccgc cccgaccaat acgcagacgg
     7081 tgcgctacga ggtgcccgcg ggctccgacc gtgttttggt gaggatggtc atccgcgacg
     7141 agcgggggga gcgggaggtt ttttcgggtt atcagaacgc cggcgcgttg gtggaggtcc
     7201 cggtcgtcat tcgaggggct tctcgcgtgc ggattttcgt caacggcgtg ttgattgagg
     7261 agaggcttct cgaatgaggc gcggcgggat cccgcgcatc gcgccgtcca ttctgtccgc
     7321 cgatttcgcc tgtctgggaa aggaagtgga gcgcatggag cgggccggag ccgattggct
     7381 gcacgtggac gtgatggatg gacatttcgt ccccaatctc acggtggggc cggtggtggt
     7441 ccggtggctt aaaaagcgca cccggctgga tatggatgtg cacctgatga tcgaggcccc
     7501 ggaaaaattc attcctgctt tcgccgaggc cggggcgtgg aacatcaccg tccacggcga
     7561 ggcttgcgcc gacccccgga gaacgcttcg gctcataaag aaatgcggct gtcgggccgg
     7621 attgagcctc cgcccgcgca ccccgattca aaaaatcgcc ccgtatctcg gggcggtgga
     7681 cacggttttg gtgatgacgg tggaaccggg gttcggcgga cagcggttca tgccggagat
     7741 gctgcccaag gtgagctggt tgcgcgaata tcagcggcgg agcggaaaga agttttggat
     7801 cgaagtggac ggcgggatca acacccagac cgctccccga gtggtccagg cgggggccac
     7861 cgtcctggtg gcgggcaccg cgatttttgg ccaacccgac ccgaaacggg ccttgcacca
     7921 gttgcgccgc gccgcgtcct ctcctgtttt gtagtccctc cctgtaaatc tccctaccac
     7981 ttgggtttct tgtagcgcct caataacgaa cgaccgtcca ggggcggttc tattttcgac
     8041 aggtcaaacc cataacggtc caaaatggtg ggagcgatgt ccgcttgctc gccgccgcgg
     8101 attattttcg gatcgttcga ggcgaggaaa acacgcggcg cggagatgtg gttttgccgc
     8161 tttttgtcaa agccatggtc ggatgtgaca tagatcaagg ttttcttttc gagatggagt
     8221 tctttcagct tggccattat tttcccgaga tgtttgtcta ttcgtacgag ggcatcgcgg
     8281 tgttccggag agccttcgcc gttcacgtgg ccgaagtggt ccaccatttt cgtgtggaca
     8341 aacaaaaaga aaggcatgtt ccggtatttt tctaaaaacg ccatggtctt tggatttgtg
     8401 cgaaaatggg aggaatcgaa ttcgtccgcc gaacgggcgc tgaaggagat gagacctttg
     8461 ttgatgatgg aatcgggatt ggcggggttc cagagcggtg gacgctcatc ggtggggtcg
     8521 tcgtcggtaa aggtccagtc cgtcggcagg atggccatga tcccggtagc gatgttctcc
     8581 cgcccgaagc gttcctggag gcgttcaaag agggtgtatc ctttggggat ttcccggtat
     8641 tcgctgttgt tgaaggcgcc cgtgatctcg tagccgtagc cggtaaagat ttccgcccat
     8701 cccgccgtgg tgtcggaaaa atggagcacg tcgatggaga caagggaccc cttttcgatc
     8761 aaacggcgaa ggtgggggag ctggtttttt gcgagggcgt cttttatgtc gtcccggccg
     8821 acggcatccc atcccaccaa gataagattg aactgcggtt ttttttcggc gccgaggagg
     8881 aaggcgatgg cgccgaacag caggaggccg cacagcagga gtcgtttcgg cgcggtccgt
     8941 gccggaaggc taatgagtgt agccaagaga cgattcaaac gcattttcat aatgaaggac
     9001 ccgtccgttt tcaatggcga caacgtcgaa acgcagaggg gatttcaccc gccggtgttc
     9061 tttgacgtat tccagggcgg ctttcacgat tttcccctgt ttgggggcgg tgacgaattc
     9121 ctccggcgcg ccgaaggcgc gggtcttgcg cgatttcact tccacgaaca ccagagcgtc
     9181 gccttcctgc atgacgaggt ctacttcccc gtagcgcgtg cggtagttcg ccgccaggaa
     9241 cacgtatcct ttcgattcca gataggactt ggcttgggtt tcggcggcgt ggcctttgtc
     9301 gagagacatc aggaacaggg ggccatctcg gcaaaaggga gaagggggga catggcctcc
     9361 accacgggcg cgaaggagcg gcggtggatg tcgcaggggc cgtgtcgccg cagggccgcc
     9421 agatgggcgg gggtgccgta ccctttgttg ttgcaaaaat cgtattgggg atattttttg
     9481 tgggcctcgg ccatccaccg gtcccgcgtg actttggcca ggatgctggc ggccgccacc
     9541 gaggcgctct ttgcgtcgcc cttcaccacg gtttcctgga cggaacgaca ctcggggatc
     9601 ttccggttgc cgtccaccag aaccaaatcg ggggaaactt tcaggtggta aagggcccgc
     9661 cgcatggcgg tgaaggtggc ctggaggatg ttgagttcgt cgatcatcgg ggcatccacc
     9721 agggccacgg aatgaaaaag agactctcgg cagatatgct cgaagagtct ctctcgggtc
     9781 tcggcggaaa gttttttgct gtcgttaagg tctttccaga cgacatccgg tctcaggacg
     9841 acggccgccg ccgccaccgg gccggcccac gggccccgcc ccgcctcgtc caccccggcg
     9901 atgatgttga agcctttttc gcgtttggtt tgatcgaagc ggtagagccg ccgggacatt
     9961 acttcgtcgg ggccggttcc gcgtccgccg ttttttgtcc ggcgggcttc tcggctttgg
    10021 ctgccggttc ggcggtctgt tcggtcgcgg aggtttcttc ctcgatgcgg gccgctttgc
    10081 cggcgagttc gcgcaggtaa tagagtttcg cccggcgtac ttttccggaa cggacgaggg
    10141 tgatgcgttc cacccggggg gcgtgcaacg agaaggtccg ctccaccccg atcccgaaag
    10201 aggttttccg caccgtgaag gtggtcgaaa ggccgtgtcc gcgcatgcgg atgacggtgc
    10261 cttcgaacgc ctggacgcgt tcgctctcgc cctccacgat ttttacgtct acccggacga
    10321 ggtcgccgac gcggaacgcc gggatgtcct ttttgagata cgatttttcg agttcttcga
    10381 tgattatgtt catggcagtc tgaaaccttt ccttgaaaga ataaaaatcc ctacaacaaa
    10441 tcggggcgct tcttccgtgt gttcttcagc gactgttcct gccgccaccg ctggatggct
    10501 ttgtggtcgc cgctcagcaa cacggagggg accgtccgtc cgcgccacgc ggcgggccgc
    10561 gtgtagtgcg gacagtccag ccgtccgcgc cagccatcgg cgaacgaatc ccaaaagagg
    10621 gagtccgctt ctttcaccac gcccggcagt gtgcgggtca ccgcgtccac cagggccatg
    10681 gccgggatct ctcctcccgt gtagaccgcc tcgcccaccg aaacttccat gtcgacccag
    10741 gccaggaggc gctcgtcgat cccttcgtaa tggccgcaaa gaacgattaa atgttttttt
    10801 agccgcaact cgcccgccag tttttgcgtg agcggtttcc cctgggggga gaggtagacg
    10861 acaaaaggtt ttttgccccg ttttttcatc gcgcccgtgg ccttgagggc gcggaaaacg
    10921 ggttccggtt tcaacaccat gccggggccg ccgccgtagg gccggtcgtc cacgctgcgg
    10981 tgtctgtctt ccgtgtgatc gcggatgtcg tgaacggcga tgcggaccag tcccttgtgt
    11041 tgagccttcc ccagcaggct ttccgaaaaa acgccgggga acattctggg aaagatcgtc
    11101 aaaacgtcga tgcgcagcgg aatgctttta ggtttcataa atttcccgca ggccgggcgg
    11161 gaggcgcaca aggatccgct tcgccgatac gtcgatttcc aaaaccgttt ctttcatggc
    11221 ggggatgaga tattcccgcg ccccgaccac cacaaagaca tcgttggcgc cggtgggata
    11281 cacgtccttc agcgttccga gcacttcacc gctttccgtc accacgtggc agccgtcgag
    11341 gtcgctcacg cggtaggagc ggtcgggaac ggtcattccg ccagttccac tccgatgcgc
    11401 tgattgtcct tggcgaaagc cgccgagacc aggcggcgga gggactggat gttgcggccg
    11461 ccttttccga tcaccttgcc tcgatcctcc ggcgccacgc gcacgtccac tcggccgcct
    11521 tccggcgact cgacccaacg gcccacgacc ttttccgggc tgtcaacaag gcactgaaca
    11581 atggacagaa caaattccaa tggggatgcc atggtaagta agggccaggg aatcaggaag
    11641 cggtctggat ctggccccag atgcccttgg tgaccagaat ggtacggagc gtgtcggacg
    11701 gctgggcgcc tttgctgatc caatatttca ggcgttccgt atcaacgacg attttttcgg
    11761 ttttttcttc cggatggtaa tgcccgatca cttccaagct ggcggcgtcc cgcgccttgg
    11821 tgccgtgaat ggcaacaatc cgaaaatgag gcttgtgggg ccggcccact ctctgcatgc
    11881 ggatgcgaac cataggattc tccttctcgg taaaaacgaa acgttatttt agcttaacac
    11941 cggggatcag tcaataaggg ttttccgtca aatttgacgg tgcgggaccg atttgataga
    12001 ctgctgtccg gctttttgaa tctcacccag cgtacatccc aaaggaaatt cggcattcct
    12061 tgaaaccgtc cccgggccca agccgcccgc ggagcggaaa aacggagcgg tctgtcttgc
    12121 gggcccggag ggccgccttg cgccgaaaca aacctgcggt cggagacgtt cgcatcaaaa
    12181 cgcgcgtgga tttgcgacag acgtcgtcac cccctgaggc cggtggtctc tccccggagc
    12241 cggcggcgcg gctattgaac gctttcgcca aaccgttttc cacctccgtg gcggccgcgc
    12301 gcacctgtta ttcctccgaa gtcgtcactc cccaggacgt tgaaaaggac gagaggtcgc
    12361 gggagcgccg ccatgcgctc gccgacagca tctatcgcgc cggacaccac accactctcc
    12421 aacacgccga gtttcagttt gtcctggaga atgtttcccg cctgttcatc tggtctttct
    12481 tgcacagtca tccgttctat aattccgaac aggtcagcca gcggtatgtc cccgtggaac
    12541 cggggcgctt ggcggtgcct cctttgccgg aaaagccgcg ggcgttgttt caggagatcg
    12601 ccgcccagct gatgacggcc tatgaacggc tgacggaact tgtttacccg tccgcggccg
    12661 gcgaatacga aaggattttt ccgctgcgca atgtccggga gccgggatgg gggaaaatgg
    12721 tccgtcgtcg cgcccaggag gcggcccgtt acgtgctgcc cctggccacg cacgcgcacc
    12781 tctatcatac cgtcagcgga ttgacgttgc accgatatca tcgcctctgt ttggcctacg
    12841 acgcgccgtt ggaacagaga acggtggtcg aaaaaatgtt ggcggaggtg cgggctttgg
    12901 acccgctgtt cgtggaccac atggaggatc cgctgtcctt ggaggagacc ccggaatacg
    12961 aactttttaa gcagttccac gagaaccggc cgccttcacg tgaattcctg gacgagttcg
    13021 accgatccct cggtggccgg atttcaaaat tggtcgacta taaggtcaac ggcgaagccg
    13081 ttttggcgca ggcggtgcgg tccgtgttgg gcgtggccca acgccgcctg gccgacgaga
    13141 cggccatcga cgccgttctg gacccgtcgc gtaaccgata tttgggcggg tcgttgaacc
    13201 tcgcctccca ctccaaactg atgcgcgcgt tgctccatat gaatttcact ttccggcgaa
    13261 agctttcgca cgcggccgac agccaggacc agcgccaccg catgacgccg gcctcccggc
    13321 ccctcttggc cggccaccag gccgtcgagc cggactacat cgtcccttct ttggtgaagg
    13381 aaaccccggc cgctttggag ttgtacggag agactttcga aaagttatgg aagaatctgc
    13441 ggcgcctgtc ggagatgggc gtgaagcaag agttcgttct ctatctgttg cccaacgccg
    13501 ttcccgtgcg gtatgaggaa tcgggcgatc tcctccatta tcaccacaaa tggtccaagc
    13561 ggctctgtta tctggcgcag gaggagattt ggcgggcctg cctggacgag gtggcgcagg
    13621 tcaaggaagt ggcccctcgc gtggcccggc acctcgggcc gccgtgcgcg cttcggtcgg
    13681 cggccggcca gcgaccgttt tgcccggagg gagaccggtt ttgcggcgtg aaagtctggc
    13741 agttgccgtt ggaatcttat cggaggctca tttgaatttt ggaatggcgc ggcgtcgccg
    13801 cgtgtggtaa attctacggg ttttgaggcg aaactcaacc gttcagggca ggaggaaaaa
    13861 tggccacatt gatcgttgtg ggggcgcagt ggggggatga gggcaagggg aagatcgtcc
    13921 atcttctggg gaagaaggcg gattacatcg tccgctatca ggggggaaat aacgccggac
    13981 ataccgtcgt tttcgacggt aaaacgttcg ttttgcacca gatcccgtcg ggcatccttc
    14041 agcccggcaa gaaatgcatc atcgccaacg gcgtggtttt ggacccggcc gccctgaaag
    14101 aggagatcct gttcctggaa tcccgcggca tccgcgtccg cgggcggctg gccgtcagcg
    14161 cctgggccca cctcattttg ccgtaccata aatacctcga cgcccatcgg gaggcgggaa
    14221 gcgacaagat cggcaccacc aaaaaaggca tcggcccggc ctattccgac aaggtgggcc
    14281 gcaccggcat ccgcctggcg gatttcatgg agcccgacct ttttcgcgag ctggtggaac
    14341 gcaatctcca ggaaaaagcg cccgtgttgg agcaggtgat ctcgttggac gctctgcgcc
    14401 gggagacgtt caaggattat gatgcgttgc ggaaattctt tgagccgttc gtggccaaca
    14461 cccccgcttt gctgcaagag gcgctggccg ccaaaaagaa cgtcttgttc gagggcgcgc
    14521 agggggcgtt gttggatgtg gatttcggca catacccgtt cgtcacctcg tcgaatcccg
    14581 tggccggggc cgcctgcgtc ggcgccgggg tcgggccggg cgccattgac gaggtcctcg
    14641 gtgtggtgaa ggcctacacc acccgcgtgg gagacggtcc ttttccgacg gagttgaccg
    14701 gggagatggg ggaattcctc cggagccgcg gccaggagtt cggcgcgacc acgggccgcc
    14761 ctcgccgctg cggctggttc gacgccgtgg tgatgcgcca cagcgtgcgg gtgaacggaa
    14821 tcacgcggct ggcgctgacg aaattggaca tcttggaggg ggtcgacccc atccgggtgt
    14881 gcgtggcgta tcggtacaag ggaaaacttc tgaaagattt ccccgcgtcg cgtcaggccc
    14941 aggcggcggt ggcgccggtg tataaggacc ttccaggttt taccggcccg gtgaaaggga
    15001 ttacgcaact caataaactc cccgtcgcgg cccgcgacta cgtgcggttt cttgagaaac
    15061 agtccggcgc gcccgtcggc ttcatctcca tgggccgcag ccgggacgag accatcctca
    15121 tcgacaaaag gttccggtgg actccctgaa cgccgtcctc gcgctggaga cgggccgcgt
    15181 tttcgtcggc cgttcgttcg gggcggtcgg ggagacctcc ggcgaggtcg ttttctgcac
    15241 ggcgctcgcc ggttatcagg aaatccttac cgacccctcc tatcaagggc agatcgtgat
    15301 gatgacctac ccgcacatcg ggaactacgg caccttcgat gccttgaacg aatcgcggaa
    15361 accgttcgcc gccgggttca tcgcgcggga attcagcgac gtggcatccc acagccggtc
    15421 gacggcgtct ttggcggaat acatgaaaaa acacggggtc gtcggcatcg acggcctgga
    15481 tacccgcgcc ctcacgcgcc accttcgaca gctgggcgtg tgccggggga tcatttccac
    15541 agggaacgtt tctccaaaaa aactgattga gcgggcgaaa aaaaccgcgc ccatggcggg
    15601 tcgggacctg gtgagcgagg tcagctgttc atcggcgtac gaatggcggg agacaggaga
    15661 agaactcgag gtcgttccgc cctcggccgg ccgtcgaaaa gtggtggtga tggatttcgg
    15721 cgttaaaagg aacaccctgg actccttggt gaggcgggga tgcgatgtca ccgtggttcc
    15781 ggcccagaca acggctcagg aaatattgac tttgcagccg tccggcgtcc tgttttccaa
    15841 tgggccgggc gatccggcgg ccgtcaccta cgccatcgag accgtgaaac ggctgatcgg
    15901 ggcctgcgaa aaagggcagg tggatgccct cttcggcatt tgtctcgggc atcaattgct
    15961 cggtctggcc ctggggggga agaccttcaa gctgaagttc ggccaccgcg gcgccaacca
    16021 tccggtgaaa gagctgagca cgggcaaggt ggaggtgacg acgcagaacc acggcttttg
    16081 cgtggacgtc gattccttgg cggcaaaaga ggtggagttg actcacatca acctgaacga
    16141 tcggacgctg gaggggtttc ggcacaaaaa attgccgatc ttcgccgtgc aataccatcc
    16201 ggaatcgagt ccggggccgc acgattcccg ctatctgttc gacaggttcg tttctttgat
    16261 gaagtaagag cggcgttcct tgaagaaagg ccggggagaa aaataaatgc ataccgtttt
    16321 gctgatcgcg tgcaagaacg aggaacggac gatcggcgcc ctcctgccgg aggcgaagaa
    16381 atacgtgaaa gacatcgtcg tgttggacgg ccgatcttcg gattccagcg cgaagatcgc
    16441 aagggagtcc ggcgttgagg tcgtcgccga cgccgggcgc gggaaggggg cggcgctccg
    16501 ccgggcgttc gaagaacgcg acggtgacgt cttcgtcacg atggacgccg acggctcgca
    16561 tgaccccaag gatatcccgg cgctgatgcg gccgatcctg gaagggcagg ccgatctggt
    16621 gatcggctcg cggttgcgcg gcggcagcga ggagttcgtc ggcgatctgg aaaaaacgat
    16681 acgggtcgtc gggtccctca tcatcacgta cgccatcaac aagcggttcg gcgcgcagat
    16741 caccgattcc caaaatggtt tccgagcgat acgggccgaa acggcgcgga aactcaaatt
    16801 gaactcgaac tcgacgacca tcgaacagga aatgctcgca aaaaccctta agatcggcgg
    16861 gcgggtggtg gaggtgccga gccacgaaaa cgcccggtcg ttcggccgat cccatatccg
    16921 ccttctgcgc gactggccga aatatctctg gtcgctgatg attgatcttg tttaagccct
    16981 tgtccataaa gtccgccgcc gatcgggcct cttccgatgg agaataaggg tcgtcctcgg
    17041 acaatactgc tcctgaaccc gccggctccc gaagtgatgg tgcgcgaagg gcgttgcatg
    17101 cagcgacggg gagcgtggac gaccgtttgg ccgcctctga cattggccac cttggcctcc
    17161 atttttctgc agcagggaca tgtcgtccgg ttgcgggatt gcccggtgga gggggtgggc
    17221 tgggaggggc tggaaagtct gttgcgagag ttcgttcccg attttgtcgt catcaacaca
    17281 gccaccccga ccatcgttaa cgatctgaga gcggccgccc tcgccaaagg cgtgtgccct
    17341 gccgtcaaga ccatcgccat cggcatccat ccttccgccc ttccgggagc gactttgaaa
    17401 ctttgtcctg atttggacgc ggtgtgcatc ggcgaacccg agcgggcggt cccggaatgg
    17461 gtcggtggaa aagtggggcc ggcgggggcg ggggttcttt ctcaagggga tcgggacacg
    17521 ggagttcgct cgacggccaa atacctggaa aatctggacg agttgccgtt tccggcctgg
    17581 cagttggtcg acaggagaaa ttatcggttg cccttctcgg cccgcgaatt ccttctgatc
    17641 aacgtcggcc ggggatgccc gcaggggtgc ctgttctgcg ccgcccggac ctactacggg
    17701 aacgcggtga ggttgcggag cgttgatcgg gtgatacggg aaatgaaacg caacgcggcg
    17761 gatttcgccg tttccgatta tcttttttgg gcggaatcgt tcacccagga aagggattac
    17821 gcggcatccc tgtgcgaggc gattctaaaa gagcgattgg acgttcggtg ggtctgtaac
    17881 gcccgcgtgg accaggtgga cggggacctg ctccggctga tgaagagggc ggggtgttgg
    17941 atgatcggtt acggggtcga atccctggcg gaagaagtcc tgcggcggaa ccgaaaaaac
    18001 gtcgttcccc ggcagatcga agaggcggtg gtgacggcaa aggccgccgg gctggcggtg
    18061 atggctcact gtgttttcgg attgccggga gaaacggaag agacattgaa ggtcacggaa
    18121 tccgggatcc ggcggttgcc ggtggatttc gtccagtact acaccgccgt gcctttcccg
    18181 ggatcggaac tctacgcgtg ggcggttcgg gaggggcggt tgttgtcgga cgatttcgtc
    18241 cgttttgaac agaacagcac cgttttgcgt ttggacggcc tgccggctga aaaaatcgcg
    18301 gggttccggc ggacggcgta cctgagattc tatctccgac cgcgtgtcat ttggaacgtt
    18361 tttcgattgg ttttgagtcg cggcggcccg gcggattttt taaggacttt tttcgacttc
    18421 ggccgttggg ctgtttcccg ctagatctgt ggggtttgcc ggtctttgaa atagggggcg
    18481 gaatttttcc gccgtggacg cgtcggcgac gtggcacgtg acgggaccgg ctggaaaaga
    18541 aacgggcagg atccgccgta aaagagtttc ggcggttttg gagtgggcga ggaccagccg
    18601 aacttttttc atttgaagat aggacttgaa ttcctcttcg ggcaggtcca gcaggcagcg
    18661 ctcgtcaggc cgtgtgcgga catattctcc gttcacgagg tcgaaagaat ccgggttctc
    18721 cctgtcaaaa aggatatggt cgggagattt aaaaggggtg agcgcggaaa gagtgatctt
    18781 cccgttctct tcggaagggg ggcctccttt ccagagttcg acgaggaccg tctcttcttc
    18841 gtttaaaaag cggttatccc aaagggattt caaggaatgg ccgaggcgga tgatgtcgcg
    18901 gggaggatcg tgggggacga agaacagctt gggccgcagg gcccaggaga aataaccgac
    18961 gacgagcatc ccatagagaa aagcggttat ctgagcggag cgtttcagcg gagaatttga
    19021 agaaaagaat cggaacagaa gcgggatgaa caacattacg ccgggccagg ggtggtattt
    19081 cggcgtgccc agccgcagaa tcac
//
LOCUS       DAOTFD010000026        19040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_1915_length_19040_cov_4.467738, whole genome shotgun sequence.
ACCESSION   DAOTFD010000026 DAOTFD010000000
VERSION     DAOTFD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 19040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19040
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1915_length_19040_cov_4.467738"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5079_03020"
     CDS             complement(<1..288)
                     /locus_tag="P5079_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY28988.1"
                     /translation="MGFSRTTARRLTSAAGLLLAAVFLYVFWPPRPPVASLRKRTPET
                     TAMMDIRARQARRKGRPWRVSRQWVPLGQISPNLQRTVLAAEDDGFYRHGGV"
     gene            complement(387..1781)
                     /locus_tag="P5079_03025"
     CDS             complement(387..1781)
                     /locus_tag="P5079_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28989.1"
                     /translation="MAHPMANDPLDPLKKIQSHLDALRRVQNIPNVNITTPHADVSNL
                     QIQFKMPGGRVFSPVPVVPDPLVEWMLEDLAAQPFHDLVTETVNMQKTKGYRPGHVPR
                     AEGLKVLKKRYDKDAFFRARVGGLWLQSRKKEEDALKLLKAETVRANPWPVVERFGFP
                     LAVWLFFLTGSGTFRGVAAQVLKESAAKGGNLEELVASAAQRAPEAENLWGLPPLTHD
                     AESARLKEEMSGLLAERDRLKQEAEEGKKRRESLEEELRKAREENQKVAHARNAAENA
                     RAAAAVRTHELETHLESLRGAESGLKKLEKQVKELEHEKARFMKEKEEWEKNGRAVRE
                     DRDRLKAELKEKQKSFQVLSKLLAGKELPIKALYQGQILFLVTRADPAPFFDVAKRAG
                     LTLLVHDGQARNAQFENFLEQAWRVFVLGAEGDFQEGALRAVRESAAPAAFLPSLDER
                     QFAALLAVTAKASL"
     gene            1938..2459
                     /locus_tag="P5079_03030"
     CDS             1938..2459
                     /locus_tag="P5079_03030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY28990.1"
                     /translation="MPQQTGEATLVLIKPDGIKKSLTGNILTKLSETRFIIVGAKVVR
                     VTTKLAEAHYQHLKDKPFFPDLIKYIQGKPYGPQYERVFALVYKGPGAIPKIREMAGA
                     TNPEEADSVSIRGAYGRITTKGVFENVIHASSSPEDAEREIKLWFSPEEIVGDLYPTK
                     KVTLPAREVLEWA"
     gene            2556..4349
                     /locus_tag="P5079_03035"
     CDS             2556..4349
                     /locus_tag="P5079_03035"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRY28991.1"
                     /translation="MRNAEKGTPMKPNNKTVVAWVEEMAKLCKPDTVVWCDGSEEEKQ
                     RLISEALRTGELEELDQKKLPGCYYHRTALNDVARTEQLTFICSPTAEGAGPTNNWMD
                     PKEAYRKLGAIFDGAMKGRPMYVVPFLMGPPGSPFVKVGVELTDSRYVVLNMRIMTRI
                     GDVAWNQLGDSSHFTRGLHSKADLDEKRRFICHFPQDNTIWSVGSGYGGNVLLGKKCL
                     ALRIASALGRKEGWMAEHMLILGIENPKGEVRYVTGAFPSACGKTNLAMMIPPAALKG
                     YKVWTVGDDIAWLRIGPDGRLWANNPEAGFFGVVPGTNSRSNPNALQTIQRDTIYTNV
                     AKKPDNTVWWEGSDDPAPAQATDWKGRPWTPASGEKAAHPNSRFTAPAAQCPCISPRW
                     EDPAGVPIDAIIFGGRRARVAPLVYQTFHWQHGVFVGASAASETTAAQQGSAVGVVRR
                     DPMAMLPFCGYNMADYLAFWLEMGKRIPNPPKIFNVNWFRTNADGQFIWPGFGDNLRV
                     LLWILDRVAGQGRAVESPIGYLPPAEALNRDGLPLSDGELNSLLSLDKTEWKTELQGV
                     GEFFQKFGDRLPKALWEEHAAAGRRINDWKF"
     gene            4449..6236
                     /gene="dnaX"
                     /locus_tag="P5079_03040"
     CDS             4449..6236
                     /gene="dnaX"
                     /locus_tag="P5079_03040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY28992.1"
                     /translation="MSYLVLARKYRPQTFEELVGQEHVSTTLINAMDAKRLAHAYIFS
                     GPRGCGKTTTARILARALNCAQGPTPHPCGQCSQCLEISSGNSPDDVIEIDGASNRGI
                     DQIRDLRDTVKYAPARSRYRIIIIDEAHQITEAGFNALLKTLEEPPPHAVFMLATTDV
                     QKFPATILSRCQRFQLKSISPAGIQQQLKKIADLEKIRISPEALLEVARSANGSLRDA
                     LSLLDQVIAYSPDGATDKDIRALLGLLPRETVRAFADVLREGVPEKTLAAVQKAVSDG
                     FDLFQLAHDLTSFWHGLLLAKSGVPDSFFANSSEAQELAGLYTLPRLERNLQIISRTT
                     EQMRRSEASRVTFELACLELSKKTMSADEILSRLEDLEKNLRDADFPTTTPGRPPAAD
                     APTLKKKVAEPAAPPPAALPTPPPLPAAPPPPAKESSLTDTDVRIAWPGILQEIGKKK
                     PALEPSVAEASWELKPQGNIVVYSSKEFHHQQILAQIPLLQKVLADKLSAAVHVQCHF
                     KPKPAPVDAAPKSLPAAEPTPEEMVAEEAEDSGFADDNSRPEPAKTTESAADNVDAEE
                     IVRQDLGAKKLLDRFPGKIKKIHPTPVTP"
     gene            6262..6864
                     /gene="recR"
                     /locus_tag="P5079_03045"
     CDS             6262..6864
                     /gene="recR"
                     /locus_tag="P5079_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRY28993.1"
                     /translation="MPKAWNLLVSSLLNLPGVGPKMAERMALHLLRHDPDSDDLLQRL
                     EEARRKLRRCSQCGDFVESDAPVPTCPLCRDTDREDSLLCVVEEIGDLQAMERSRAFR
                     GRYHVLGGVLSALDGVGPEELRIHELLQRIRSGKITEVILATNPTAEGETTAAYLADL
                     LKPLGVQTTRLAYGLSSGSAIQYADEFTLARALEGRRPLS"
     gene            6809..8302
                     /locus_tag="P5079_03050"
     CDS             6809..8302
                     /locus_tag="P5079_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY28994.1"
                     /translation="MLMNSPSHGPLRDDGPSLNVAGRGRNRSVITAVPAVMIALGLSM
                     PPAEAEEGAGLESCVRIALANNADFFVAQKDADLNRARARIATLQLFPAAALKAEETR
                     GRADELNGTPSFVERLYGVQATQTLFQGGHRWATQRQARTKADIGSLEVARQRNLLIF
                     QVEEAYWRLSALENAKAAYRETSLALQDSLEKAARHELSDSPRARIEILSTRAQNREC
                     EVALAETEEAILQARSRLLDLLGQNLPSFPADPLPDVKKLSAEEDECLRLGRAHRPEP
                     ALAEKEARAAEWGRLAGRSALYPKVDLHGFYGRSGAAYDQSEPFHYQRDWNAGVRASW
                     VFLGNTGGYSSSKEKTSPKLGESSRTETQTQALRLSFADAFGPSLDNAEAQKAHWLGQ
                     RRLEKTRRHTDEDIRLTCRKLENAWKKTQSAQARLEQAQQEFTDTRYLLQEDKAHLGD
                     IAAAVNRVGSARADLSEARANYRIAVADLNRAIGIPGHFKTEGDLKE"
     gene            8299..9408
                     /locus_tag="P5079_03055"
     CDS             8299..9408
                     /locus_tag="P5079_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY28995.1"
                     /translation="MTVTPKRQSPHKTILSLFGVLLLLLAGASCLFRRWTGRQPPAEA
                     SPIAKKILPVKTYRIRPQKLEGVLLRNGTIRPHRETNLQFGVAGRLVKFDVEKGQFVR
                     KDALLAQLDPSEAKSFLDGVELDFRRASLKYYKDRSIDKLEFDRAKLRYQQAKLEYEK
                     TSLRAPHSGFLVEKSALPGEHVESGTVVGKLVDKSRVFVELSLTEQDIAYLQEGQGVG
                     VTVDALPDFRAEGKVRSITPYLEGNARSFTVKVTLPENPGEALTPGMYARCQIHRYIK
                     DAALTVPTEAVVDTLGKQARVFVVDAQNRAFLRTVPVLFSSDKEMEIGEVSEGDMVIL
                     APSLLISDGAKVEVSGVFDPAASQSVPPTKEADHP"
     gene            9405..9779
                     /locus_tag="P5079_03060"
     CDS             9405..9779
                     /locus_tag="P5079_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY28996.1"
                     /translation="MSAGKILLADDDRYITQILEDMLVREGYSVSVVHDGAAALEAYR
                     KENPDLLILDVSMPLKDGLSVCEEIRRTDQDVLILMLTAQRAQVNTVQGLTVGADDYM
                     TKPFGPKELLARIRSLFRRGHK"
     gene            9776..10477
                     /locus_tag="P5079_03065"
     CDS             9776..10477
                     /locus_tag="P5079_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY28997.1"
                     /translation="MRPPVSPAKNHILLVEDDRDIQTLLKYNLEREGYAVALAGDGES
                     AVSLFRRQKPRCVILDLMLPRVDGLEVCRLLRTDDRRVPILMLTAKNTEADKIVGLEM
                     GADDYVTKPFSVREVLSRVKALLRRTAPDAAPASRTAGALAVDFEKYELLLAGKTVPL
                     TPKEFELLKILWSANGKAVERDSLLERVWGYDRAADIDTRTVDQHVARLRAKLGREKR
                     RVVTVKNVGYRFISE"
     gene            10474..11580
                     /locus_tag="P5079_03070"
     CDS             10474..11580
                     /locus_tag="P5079_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY28998.1"
                     /translation="MKFDLGGFHFQIDRSATPPPTDSPVPIAPPRQTDLLAGIDEGLL
                     ALDHRQRIVFCSNAMRPLLNRPPSESVGRPLWEVLRHRPVQQIVEQSLKTGETARTDL
                     APTGADGSLWSVIARPSPVSGAAGVLLLFKDVTEIRRLERLRKEFVANVSHELKTPLT
                     ALKAALETLLDGAWEDRRVAREFLQTAQSNVERLHRLIDDLLLISRLERPDSARTAPG
                     KCSLSEAMRKAAASVLPLADKKRIHLETVVPDGPAEAALSEDELIQVFVNLLDNAVKF
                     TPSGGTVSFSARLNASAAEVRVKDTGIGIPAEDLPRIFERFYRVDKSRSPERGGTGLG
                     LAIVKHILENRNGAVRVESSAGRGSEFTVTFPTL"
     gene            11693..11860
                     /locus_tag="P5079_03075"
     CDS             11693..11860
                     /locus_tag="P5079_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY28999.1"
                     /translation="MKEKQTLETFRAVLRTTEKQLNELLRDIEQNDVSPETVDLQVRQ
                     IEKCLRNIRAA"
     gene            11890..12714
                     /locus_tag="P5079_03080"
     CDS             11890..12714
                     /locus_tag="P5079_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY29000.1"
                     /translation="MKVPAALLLALFQTAPSMTAAGITIKGSDTMVILNQRWAENYME
                     KNPGVVIQVTGGGSGTGFTALINGSTDICAASRPMKEEEFRALEKKYGSRGVEIPVAQ
                     DGVAVYLNETNPVNELTLAQIRDIYTGKITNWKSVGGRDARIILYSRENNSGTYMFFM
                     EFVLRNKDFSPYAMSLPGTAAVANATSRDKNSIGYGGSAYAKHIKFCAVKADTDSPAI
                     EPTEENILSGRYPVCRNLFFYTLRKPRGPLEQFISWVMGPEGQEIVAQTGYFPVKR"
     gene            12711..13664
                     /gene="pstC"
                     /locus_tag="P5079_03085"
     CDS             12711..13664
                     /gene="pstC"
                     /locus_tag="P5079_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRY29001.1"
                     /translation="MKPSSLSHSVSEKIIETFIHLSAAVSIVGLVLIFIFIGKESLPV
                     FTSPEIRREASLSKIFLPQSPEPGEEKVFRWQPVSVVPKYSLLPLVVGTLKATAVALL
                     FAIPLALAAALFTSEFAPSWLREWVKPVIELLAGIPSVVMGFFALIVMATYVQELFGL
                     DFRLNAISAGIALGLAVVPVVYTMAEDALSAVPQSYREGSIALGATPWQTAWRVVLPA
                     AFPGIFAACILGLGRAIGETMIVLMASGNAAVSSWSPTDPFRSLSATIAAEMGEVVRG
                     STHYHVLFFIGVLLFLFTFFINYLGQLFVERLRQKLSGTDK"
     gene            13690..14601
                     /gene="pstA"
                     /locus_tag="P5079_03090"
     CDS             13690..14601
                     /gene="pstA"
                     /locus_tag="P5079_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRY29002.1"
                     /translation="MNRRWIDLFSRLFTLLTALACFSLVAGVALILGDVVLNGLKHLS
                     WEFVSTAPKTGMTEGGIFPALFGTVFLVLLMTVAVVPLGVSTAIYLQEYAPAGSRWVK
                     LIRMAIQNLAGVPAIVFGLFGLGFFVDFVGKGIDRFFFDGALTFGQPAILWASLTLAL
                     LTLPTVVVATEETLRVVPRSHREVAYSLGATRWQMVRRVVLPQAVNGILTGTILAVSR
                     GAGEVAPILFTGAAYYLPYLPTHLNQQFMVLNYHVYVMATQSPDVDATLPLLYSTVLV
                     LLMLTFALNFAAIIIRSRLRRRLKAGR"
     gene            14603..15376
                     /locus_tag="P5079_03095"
     CDS             14603..15376
                     /locus_tag="P5079_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRY29003.1"
                     /translation="MTPPPALQEMLRVEALSVKTKNATLLDRVSLNVREREILSIIGP
                     AGSGKTTFLRCLNRLIDLEGGLSVSGKVLLGEQDVFDDAVDLAALRRRIGMVFALPTP
                     LPLSIRDNLRLGLRFQKSSGPGTLEERIETCLRAASLWDEVKDRLGESAQKLSGGQQQ
                     RLCLARTLMVRPEILLLDEPCSGLDPISTAQIEEALQSLKDQMPVVLVTNNVKQASRT
                     SDRTAFFLQGELVELDDTGRLFTAPRDPRTADYVSGRFG"
     gene            15382..16182
                     /gene="pstB"
                     /locus_tag="P5079_03100"
     CDS             15382..16182
                     /gene="pstB"
                     /locus_tag="P5079_03100"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRY29004.1"
                     /translation="MAATSVHQGNPPAALETPKIAVQDLNLWYGSFHALKNVRVDVLP
                     RKITAFIGPSGCGKSTVLRTFNRMNDLIKGVRVTGKVLIDDHNIYEEDTDLIRLRKRV
                     GMVFQRPNPFPISVFENVAYGPRIHGETRRRRLEELVEKSLRATALWDDLKDRLSTPA
                     LGLSPEQQQRLCLSRLLAVEPDVLLMDEPCSALDPIATARIEELAVELSKDFTIVIVT
                     HNMQQAARISHVTAFFLLGELLECGETSQIFTNPKNEKTEQYITGRFG"
     gene            16204..16875
                     /gene="phoU"
                     /locus_tag="P5079_03105"
     CDS             16204..16875
                     /gene="phoU"
                     /locus_tag="P5079_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRY29005.1"
                     /translation="MQRHFEENLNALKEKIIRMGSLAEEMIQKSVRALMERNPSITQD
                     VFDMEREVNLLHVEVDDRCVKLLALTQPMAVDLRLIAAAMKINTDLERIADQAVNVCQ
                     TCYYHLLKETPVPQANIIPRMAEISQSMLKNSLDAFSKKDIELAKKVLLQDEEEDRLK
                     AETLSELIELIKREPDHSKQFVDLILIAKNMERISDHATNIAEDVIFMVLGVDIRHHI
                     EEKRP"
     gene            complement(17102..17941)
                     /locus_tag="P5079_03110"
     CDS             complement(17102..17941)
                     /locus_tag="P5079_03110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY29006.1"
                     /translation="MRKIFFLLTASACLFGETFLGALDVDGLNLNKVFSGTKKVAQGM
                     MKVSDKDEIALGREVASYLCARYGLVRDPALTKYVGLVGRTVAQGCDRPEIPYHFGIL
                     NTDEVNAYAGPGGYIFITKGLLRGLKDESELAGALGHEIAHVARRHVAKEIQKGNLIQ
                     GGVELASAREESPEAFKAVSDFSIKLLFKGFSRGDERESDQKALSYAATAGYTPAGFQ
                     NLLNRLAAEKSGETRLKALNKSHPPSSDRARLVQREIETEKWDLSLPANEPRFLAAVA
                     ALK"
     gene            complement(17938..18255)
                     /locus_tag="P5079_03115"
     CDS             complement(17938..18255)
                     /locus_tag="P5079_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29007.1"
                     /translation="MKNAEVKSEKWRRRAWAVAVLLAGLMAVPPSGTGFDFKKLRPSA
                     DKKLSASQPTGVAGVRGLDEPGEEGDLSARDPEALAWLEAQPVSPDDLKTFQSSGGLK
                     KAR"
     gene            complement(18255..>19040)
                     /locus_tag="P5079_03120"
     CDS             complement(18255..>19040)
                     /locus_tag="P5079_03120"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY29008.1"
                     /translation="VPRKAEDLRSLPGIGRYTAGAVLSFSFNRPEPVVDGNVVRVLSR
                     VYGIEEDVKKAATQEHLWGLAWKLVPRSGARLSGPHAFNSAIMDLGATVCQSASPECL
                     LCPLRKTCWAYRHGAVERLPMAGEPQGRRVVHLNVGAVRSARRWLMLQRPAEGLYGGL
                     WEFPCVETRTAVRSAEELLEKKLGVALVPVGALPLFRHVLSHRDLQVRSFVFDAGGDV
                     PKVAGGKWFDLEEIARSAISTLTRKILFSLRRVQPSLKEVSLI"
BASE COUNT         3705 a         6723 c         5420 g         3192 t
ORIGIN      
        1 cacgccgccg tgccgataga acccgtcgtc ctccgccgcc aggacggtcc tctggaggtt
       61 cggggagatc tgccccagcg gcacccattg ccgggagacg cgccacggcc ggccctttcg
      121 gcgcgcctgg cgcgcgcgga tgtccatcat ggcggtggtc tcgggggtcc gttttcgaag
      181 ggacgcgacg ggcgggcggg gcggccaaaa cacatacagg aacaccgcgg ccaggagaag
      241 gccggccgcc gaagtcaaac gccgtgcggt ggtgcgagag aatcccatct atccggccgt
      301 ccttccgtcc gattttccga aatcattttc gcgtttcatg gaacggctca aaccgccgag
      361 ggggaggggg cgcggccggg aaaaacttac agcgaggcct tggcggtgac ggccagaagc
      421 gccgcgaact gccgctcgtc cagcgagggg aggaacgccg ccggcgcggc cgactcgcgc
      481 acggcgcgga gcgccccttc ctggaaatcg ccctccgccc ccagcacgaa gacgcgccag
      541 gcctgttcga ggaaattttc gaattgggcg ttcctcgcct ggccgtcgtg caccagcaac
      601 gtgagaccgg cccgcttggc gacgtcgaaa aacggcgcgg ggtcggcccg ggtcaccagg
      661 aaaaggatct gcccctgata gagcgccttg atcggcaatt ctttgccggc cagcagtttc
      721 gaaagcacct gaaaagattt ctgtttctct ttcaactccg ccttcagccg gtcgcggtcc
      781 tcgcgcaccg cgcggccgtt cttttcccac tcctcttttt ccttcatgaa ccgcgccttc
      841 tcgtgttcga gttccttcac ctgtttttcc agttttttca gccccgattc cgccccgcgc
      901 aggctttcca gatgggtttc cagttcgtgc gtccggaccg cggcggccgc gcgggcgttt
      961 tcggccgcgt tgcgggcgtg cgcgaccttc tgattctcct cccgcgcctt ccggagttcc
     1021 tcctccagcg attcgcgccg tttcttcccc tcctccgcct cctgcttcag acggtcgcgt
     1081 tccgccagca ggccggacat ctcctccttc agccgcgccg actccgcgtc gtgcgtcagg
     1141 ggcgggaggc cccacagatt ctccgcctcc ggcgcccgct gcgcggcgga agcgacgagc
     1201 tcctccaggt tccccccctt ggccgccgac tccttcagga cctgggcggc caccccacga
     1261 aacgtcccgc tgccggtcag aaaaaaaagc cacaccgcca gggggaaccc gaaccgttcc
     1321 accaccggcc agggattggc gcgcacggtc tcggccttga gaagtttgag ggcgtcctcc
     1381 tcttttttgc gcgactgcag ccacaacccg cccacccgcg cccggaaaaa agcgtccttg
     1441 tcgtatcgct tcttcaagac cttcagcccc tccgcccgag gcacgtggcc cggccggtac
     1501 cccttcgtct tctgcatgtt caccgtttcc gtcaccaggt cgtggaacgg ctgggcagcg
     1561 aggtcttcca gcatccactc caccaacgga tcggggacca ccggcacggg gctgaacacc
     1621 cgcccgcccg gcattttgaa ctggatctga aggttggaga cgtccgcgtg gggggtggtg
     1681 atgttcacgt tgggaatgtt ctgcacgcgc cgcagggcgt ccaagtggga ctggattttt
     1741 ttaagcgggt ccaaagggtc gttggccatg gggtgtgcca cagtttagca tttcccgccg
     1801 ggggagacac ggagagcgct ccgccggcgc cgggcctcta tggaatccgt tgacacggcc
     1861 gtcgagggtt tggtataaac ttcggcattc aatcgtcatg cgcggcggcg tccaatccgc
     1921 gggaaacagg aaatcctatg ccccaacaaa ccggtgaagc caccctcgtt ttgatcaaac
     1981 ccgacggcat caagaaatcg ctgacgggca acatcctcac caagctgtcc gagacgcgtt
     2041 tcatcatcgt cggcgccaaa gtcgtccgcg tgaccaccaa actggccgag gcccactacc
     2101 agcacctgaa agacaaaccc tttttccccg acctcatcaa atacatccaa ggaaagccct
     2161 acggcccgca gtatgagcgg gtgttcgcgc tggtctacaa aggccccggc gccatcccca
     2221 agatccgcga gatggccggc gccaccaacc cggaagaagc cgactccgtc tccatccggg
     2281 gcgcctacgg ccgtatcacc accaagggtg tctttgagaa cgtcatccac gcgtcctcct
     2341 cccccgaaga cgccgaacgc gaaatcaagc tgtggttcag cccggaggaa atcgtcggcg
     2401 acctgtatcc gacgaaaaaa gtgaccctgc cggcgcgcga agtcctcgag tgggcgtaag
     2461 gtagccgcgc ccgccgccct cgaagaaatt ccccctgcgc accgttctct ccaaggacac
     2521 gaggaacgct atttttattt tcctataata aaaggatacg caacgccgag aaagggactc
     2581 ccatgaagcc gaacaacaaa accgtcgtcg cctgggtcga agaaatggcc aaactctgca
     2641 agccggacac cgtcgtgtgg tgcgacggct cggaggaaga gaaacaacgt ctgatttcgg
     2701 aagcgctccg gaccggagag ctggaagaac tggaccagaa aaaactgccg ggctgctatt
     2761 accaccggac cgcgctcaac gacgtggccc gaacggaaca actcacgttc atctgctccc
     2821 ccaccgcgga gggagccgga ccgaccaaca actggatgga cccgaaagag gcctaccgca
     2881 aactcggagc catcttcgac ggcgccatga agggccgccc gatgtacgtg gtcccgttcc
     2941 tgatgggccc ccccggctcc cccttcgtca aagtgggcgt ggaactcacc gacagccggt
     3001 acgtggtgtt gaacatgcgc atcatgacgc gcatcggcga cgtggcctgg aatcaactgg
     3061 gcgattcgtc ccatttcacc cgaggactgc acagcaaagc cgatctggac gagaaacgcc
     3121 gcttcatctg ccatttcccc caggacaaca ccatctggag cgtgggctcc ggttacggcg
     3181 gcaacgtgct tttggggaaa aaatgcctgg ccctgcgcat cgccagcgcc ctcggacgca
     3241 aagagggctg gatggcggaa cacatgctca tcctcggcat cgaaaacccg aagggcgagg
     3301 tgcgctacgt caccggcgcc tttcccagcg cctgcggcaa gaccaacctc gccatgatga
     3361 ttcccccggc ggcgttgaag ggatacaagg tctggaccgt cggagacgac atcgcctggc
     3421 tgcgcatcgg ccccgacggt cgtctctggg ccaacaatcc cgaggcggga tttttcggcg
     3481 tggtgcccgg caccaacagc cgctccaacc ccaacgccct gcaaaccatc caacgcgaca
     3541 ccatctacac caacgtggcc aaaaaacccg acaacaccgt ctggtgggag gggagcgacg
     3601 accccgcccc ggcccaagcc accgattgga aaggccggcc ctggacgccg gcgtccggcg
     3661 aaaaagccgc ccatcccaac agccggttca ccgctccggc cgcgcagtgc ccctgcatat
     3721 cgccccgctg ggaagacccg gccggggtgc cgatcgacgc catcatcttc ggcggccgac
     3781 gcgcccgggt ggcgccgctg gtgtatcaga cgttccactg gcagcacggc gtgttcgtcg
     3841 gcgcctccgc cgcctccgaa accaccgccg cccagcaggg cagcgccgtg ggcgtggtcc
     3901 ggcgcgatcc catggccatg ctccccttct gcggctacaa catggcggac tacctcgctt
     3961 tctggctgga gatggggaaa cgcatcccga atccgccgaa aatattcaac gtcaactggt
     4021 tccgcaccaa cgccgacgga caattcatct ggccggggtt cggcgacaac ctccgcgtcc
     4081 tcctctggat cctggaccgg gtggccggcc agggccgggc cgtggaatcg cccatcgggt
     4141 acctgccccc cgccgaggcc ctcaaccgcg acgggctccc cctctccgac ggggaattga
     4201 attcgctcct ttccctggac aaaaccgagt ggaaaacgga acttcagggc gtgggcgagt
     4261 tcttccagaa gttcggggac cgtcttccga aagccctctg ggaggaacac gcggccgccg
     4321 gccgccggat caacgactgg aaattctgac caatccctct cttcccctcg gccggtttga
     4381 cctgccggcg ttttaattgg tagagtaacg gcggatttcc gcatcgccga gcaccccgag
     4441 aggtttccat gtcctatctt gtcctggccc ggaagtaccg tccccagacg ttcgaagagc
     4501 tggtggggca ggaacacgtc tccaccaccc tcatcaacgc catggacgcc aaacgcctgg
     4561 cgcacgccta cattttttcc ggaccgcgcg gctgcggcaa aaccaccacc gcgcgcatcc
     4621 tggcccgggc cctgaactgc gcccagggcc ccaccccgca cccctgcgga cagtgttccc
     4681 aatgccttga gatctcctcc ggcaactccc ccgacgacgt catcgagatc gacggcgcct
     4741 ccaaccgagg catcgaccag atccgcgacc tccgcgacac cgtcaaatac gcccccgccc
     4801 gcagccggta ccgcatcatc atcatcgacg aggcgcacca gatcaccgaa gccgggttca
     4861 acgccctcct caagaccctg gaagaaccgc cgccgcacgc cgtcttcatg ctggccacca
     4921 cggacgtgca gaaatttccg gccaccatcc tttcccgctg tcagcgtttt cagctcaaat
     4981 ccatctcccc ggccggcatc caacagcaac tgaagaagat cgccgacctg gagaagatcc
     5041 gcatctcccc cgaggccctt ctggaggtgg cccggtccgc caacgggtcc ctgcgcgacg
     5101 ccctctcgct gttggatcag gtgatcgcct attcacccga cggcgccacg gacaaggaca
     5161 tccgcgccct gctggggctt ctcccccggg aaaccgtccg cgccttcgcc gacgttctcc
     5221 gtgaaggcgt cccggaaaaa acgctggcgg cggtgcagaa ggcggtgtcg gacgggttcg
     5281 accttttcca actggcccac gatctgacgt cgttctggca cggactgctc ctcgccaaga
     5341 gcggcgtgcc ggacagtttc ttcgccaact cctcggaggc gcaggaactc gccggcctct
     5401 acaccctgcc gcggttggaa cggaatttgc agatcatctc ccgcacaacc gaacagatgc
     5461 ggcgcagcga ggcgtcccgc gtcaccttcg aactcgcctg tctcgagttg tccaagaaaa
     5521 ccatgtccgc ggacgaaatc ctgtcccgcc tggaggacct cgaaaagaac ctccgcgacg
     5581 cggactttcc gacgacgacc ccggggcgcc ccccggccgc cgacgcgccg acgcttaaaa
     5641 aaaaagtagc tgagccggcg gcgccgcccc ccgcggccct tcccacgcca ccgcccctcc
     5701 cggccgcccc cccgccgccc gccaaggaat cctccctgac ggacaccgac gtgcgcatcg
     5761 cctggccggg catcctccag gagatcggaa agaaaaaacc cgcgctggaa ccttccgtcg
     5821 ccgaggcgtc ctgggaactg aaaccgcagg ggaacatcgt cgtttactcg tcgaaagaat
     5881 tccatcatca gcagatcctg gcgcaaatcc cgctcctgca gaaagtcctg gcggacaaac
     5941 tctccgccgc cgtccacgtg cagtgccact tcaaaccgaa acccgccccc gtggacgccg
     6001 ccccgaaatc tctccccgcc gccgaaccca cgccggaaga aatggtggcg gaggaagccg
     6061 aagactccgg cttcgcggac gacaacagcc ggcccgaacc cgccaaaacg accgaatccg
     6121 ccgccgacaa cgtggacgcg gaggagattg tccggcagga cctcggcgca aaaaaattat
     6181 tggaccggtt ccccggaaaa atcaagaaga tccatccgac gccggtcact ccctgaattt
     6241 ttccggcccg ccccgaaaac cgtgcctaaa gcctggaacc tcctggtctc ctccctgctg
     6301 aacctgcccg gcgtcggccc caagatggcc gaacggatgg ccctgcacct tttgcgccac
     6361 gaccccgatt ccgacgacct cctgcagcgc ctggaagaag cccgacggaa actccgtcgc
     6421 tgctcccagt gcggagattt cgtcgaaagc gacgcccccg tccccacctg ccccctctgc
     6481 cgggacacgg acagggaaga ctcccttctc tgcgtggtgg aggagatcgg cgatctgcag
     6541 gccatggaac ggagccgggc gttccgcggc cgataccatg tcctcggcgg ggtgctgtcg
     6601 gcgttggacg gcgtcggccc cgaagaactg cgcatccacg agctgttgca gcgcatccgt
     6661 tccggcaaga tcacggaggt catcctggcc accaacccca cggcggaagg ggagaccacc
     6721 gccgcctacc tggccgacct tctcaaaccg ttgggggttc agaccacccg gctggcgtac
     6781 ggactctcct ccggcagcgc catccagtat gctgatgaat tcaccctcgc acgggccctt
     6841 gagggacgac ggcccctctc ttaacgtggc gggacgggga cggaaccgct ccgtcatcac
     6901 cgccgtcccg gcggtgatga tcgccctggg cttatcgatg cccccggcgg aagcggaaga
     6961 aggcgccggg ctggaatcct gcgtgcgcat cgccctggcg aacaacgcgg attttttcgt
     7021 cgcccaaaaa gacgccgacc tgaaccgcgc ccgcgcccgc atcgccaccc tgcaactttt
     7081 ccccgccgcc gccctgaagg ccgaagaaac gcgcggtcgc gcggacgaac tcaacggaac
     7141 accgagcttc gtggaacgcc tctacggcgt gcaggccacg caaacccttt tccagggagg
     7201 ccaccgctgg gccacccaac ggcaggcccg gaccaaagcc gacatcggct ccctggaagt
     7261 ggcccgccaa cggaacctcc tgattttcca ggtggaagaa gcctactggc gtctttcggc
     7321 gctggaaaac gccaaagccg cctatcggga gacctccctc gccctgcagg attctctgga
     7381 aaaagccgcc cggcacgagc tgagcgattc tccccgggcg cgcatcgaga tcctctccac
     7441 ccgcgcccag aaccgggaat gcgaggtggc cctggctgaa acggaagaag ccatccttca
     7501 ggcgcgcagc cgcctgctcg acctcctcgg ccaaaacctt ccgtccttcc ccgccgaccc
     7561 cctgcccgac gtcaaaaaac tttccgcgga ggaagacgaa tgcctccgtc tgggccgcgc
     7621 ccaccggccg gaaccggccc tcgccgaaaa agaggcgcgc gcggccgaat ggggacggct
     7681 cgccggacgg agcgctttgt accccaaggt ggacctgcac ggtttctacg gccgttcggg
     7741 cgcggcctac gaccaaagcg aaccgttcca ttatcaacgc gactggaacg ccggcgtccg
     7801 cgcctcctgg gttttcctcg gcaacaccgg cggctattcc agttccaaag aaaagaccag
     7861 tcccaaactg ggcgaatcca gccgcaccga aacacagaca caggccctcc gtctctcctt
     7921 cgccgacgcc ttcggcccct cgctggacaa cgccgaggcg cagaaagccc attggctggg
     7981 acaacgccgg ctggaaaaaa cgcggcggca taccgacgaa gacatccggc tgacgtgccg
     8041 caaactggaa aacgcctgga agaagacgca gtccgcccag gcgcgtctcg aacaggcgca
     8101 acaggagttc acggacacac gctacctcct tcaggaagac aaggcccatc tgggagacat
     8161 cgccgccgcc gtcaaccgcg ttggatccgc ccgcgccgac ctgtccgagg cccgggccaa
     8221 ttaccgcatc gccgtcgccg acctcaaccg ggccatcggg atccccggcc atttcaaaac
     8281 ggaaggagac ctcaaggaat gaccgtcaca ccgaaacggc aatcccccca caaaacgata
     8341 ctgtccctct ttggagtcct gctcctgctg ctggccggcg cctcctgcct cttccgccgc
     8401 tggaccggac gccagccccc ggccgaggcc tcgcccatcg cgaaaaaaat cctgccggtg
     8461 aaaacctacc gcatccgtcc gcagaagctg gagggggtcc ttctgcgcaa cggcaccatc
     8521 cgcccccatc gggagacgaa ccttcagttc ggcgtggccg gccggctcgt caagttcgat
     8581 gtggaaaaag gccagttcgt ccggaaggac gcgctcctgg cgcaactgga cccttccgag
     8641 gccaaaagtt tcctcgacgg cgtggaactc gacttccgcc gcgcctccct caaatattac
     8701 aaggaccgct ccatcgacaa gctggagttc gaccgcgcca aactgaggta tcagcaggcc
     8761 aaactggaat acgaaaagac ctccctccgg gcgccgcaca gcggattcct ggtggagaaa
     8821 tcggcgctgc cgggagagca cgtggagtcc ggcaccgtcg tcggaaaact ggtggacaag
     8881 agccgcgtgt tcgtggagct ctccctcacg gaacaggaca tcgcctacct ccaggaaggc
     8941 cagggcgtgg gggtgacggt ggacgccctg ccggatttcc gcgccgaggg caaggtgcgg
     9001 tccatcaccc cctacctgga ggggaacgcg cgctcgttca cggtgaaggt cacgctgccc
     9061 gaaaacccgg gggaggccct cacccccggc atgtacgccc gctgccagat acaccgctac
     9121 atcaaggacg ccgccctcac cgtgccgacg gaggccgtgg tggacacgct cggcaaacag
     9181 gcccgcgtgt tcgtggtgga cgcgcagaac cgagccttcc tgcgcaccgt gccggtcttg
     9241 ttctcctccg acaaggagat ggagatcggc gaggtgtccg agggagacat ggtcatcctc
     9301 gccccctccc tgctgatctc ggacggcgcc aaggtcgagg tgagcggggt gttcgatccg
     9361 gccgcctctc aaagcgttcc cccgacgaag gaagccgacc acccatgagc gccggaaaaa
     9421 tcctgctggc cgacgacgac cgctacatca cccaaatcct cgaggacatg ctcgtacgcg
     9481 aggggtattc cgtctccgtg gtgcacgacg gggccgcggc cctggaggcg taccgaaagg
     9541 aaaacccgga cctcctcatc ctcgacgtct ccatgcccct caaggacggc ctgtccgtct
     9601 gcgaggaaat ccggcggacg gaccaggacg ttttgatctt gatgctcacg gcccagcggg
     9661 cgcaggtcaa cacggttcag gggctcaccg tcggggcgga cgattacatg accaaacctt
     9721 tcggccccaa agaactcctg gcgcggatac gctccctctt ccgccgagga cataagtgag
     9781 accgcccgtg tccccggcaa aaaaccacat cctcctcgtc gaagacgacc gggacatcca
     9841 gaccctcctg aaatacaatc tggaacggga aggctacgcc gtggccctcg ccggcgacgg
     9901 cgagtccgcg gtctcccttt tccgccggca gaaaccgcgc tgcgtcatcc tggacctgat
     9961 gctcccccgg gtggacggac tggaagtctg ccggcttctg cggaccgacg accggcgcgt
    10021 gccgatcctc atgctcaccg ccaagaacac cgaggccgac aagatcgtcg gactggagat
    10081 gggggcggac gactacgtca ccaaaccgtt ttccgtccgg gaagtccttt cccgggtaaa
    10141 ggccctcctg cgccgcaccg cgccggacgc cgcccccgct tcgcggacgg cgggggccct
    10201 ggccgtggat tttgaaaaat acgaactgct cctggccgga aaaaccgttc ccctcacgcc
    10261 gaaagaattc gaactgttga agatcctctg gtcggccaac ggcaaagccg tggagcggga
    10321 ctcgctcctg gagcgcgtgt ggggctacga ccgcgccgcg gacatcgaca cccgcaccgt
    10381 ggaccagcat gtagcgcgcc tgcgcgccaa actcggccgc gaaaaaagac gcgtcgtcac
    10441 ggtcaaaaac gtcggatacc gtttcatctc cgaatgaaat tcgatctcgg cggtttccat
    10501 ttccagatcg accgctcggc cacgcccccg ccgacggatt cgccggtccc gatcgcgccg
    10561 ccccgacaaa ccgaccttct ggccggcatc gacgaggggt tgctggccct ggaccatcgc
    10621 caacgcatcg ttttctgcag caacgccatg cgcccgctct tgaaccgccc gccgtccgaa
    10681 tccgtcgggc ggccgctgtg ggaggtgctc cgccaccggc cggtgcaaca gatcgtggaa
    10741 caatcactga agaccgggga aaccgcccgc acggacctgg cccccaccgg ggccgacggg
    10801 tccctttggt ccgtcatcgc ccgcccctcc ccggtgagcg gcgccgccgg cgtcctcctc
    10861 cttttcaaag acgtcaccga gatccgccga ctggaacgtc tgcggaaaga attcgtggcc
    10921 aacgtctccc acgaactcaa gacgccgctc acggcgctga aggccgccct ggaaaccctc
    10981 ctggacgggg cctgggaaga ccgccgcgtc gcccgggaat tcctccagac ggcccagtcc
    11041 aacgtcgaac gtctccaccg gctcatcgac gacttgctgc tcatctcgcg gctggaacgg
    11101 ccggactccg cgcgaacggc cccggggaaa tgctccctgt cggaagcgat gcgcaaggcc
    11161 gccgcctcgg tcctccccct ggcggacaaa aaaaggatcc acctggaaac cgttgttccc
    11221 gacggccccg cggaggcggc gctttccgag gacgaactga tccaggtctt cgtgaacctc
    11281 ctggacaacg ccgtcaaatt caccccttcg ggcggcacgg tgtctttttc ggcgcgcctc
    11341 aacgcctccg ccgccgaggt gcgcgtcaaa gacaccggca tcggaatccc cgccgaggac
    11401 ctcccgcgga tcttcgaacg gttctaccgc gtggacaaat cccgttcccc ggaacgaggc
    11461 ggaacgggac tgggcctggc catcgtgaaa cacatcctgg aaaaccgaaa cggcgcggtg
    11521 cgcgtcgaaa gttcggccgg gcgagggtcc gaattcacgg tcacttttcc caccctttaa
    11581 agccgccccg gccggacgtc acacaaacgt cacacacctt ttaccccacc gctatcggtc
    11641 ctgcgcccgg caaatgctat ttttttgacg gacaaacaga gcggaggccc tcatgaagga
    11701 aaaacagacc ctggaaacgt tccgcgcggt gctgcggacg accgaaaaac aactgaacga
    11761 actcctgcgg gacatcgaac agaacgacgt ctcccccgaa acggtggatc tgcaggtgcg
    11821 acaaatcgaa aaatgtctcc ggaacatccg ggcggcctga cccgccccgc cgccgtgcga
    11881 acgcgactct tgaaggttcc ggcggccctc ctgctggcgc ttttccaaac ggccccctcg
    11941 atgaccgccg ccggcatcac gatcaagggt tccgacacca tggtcatcct gaaccaacga
    12001 tgggccgaga actacatgga aaaaaatccc ggggtcgtca ttcaggtcac gggaggcggt
    12061 tccggcaccg gctttacggc gctcatcaac ggctcgacgg acatctgcgc cgcttcccgg
    12121 cccatgaaag aggaggaatt ccgcgccctc gaaaaaaaat acgggagccg gggggtggaa
    12181 atccccgtcg cccaggacgg cgtcgccgtc tatctcaacg aaacaaaccc cgtcaacgaa
    12241 ctcaccctgg cccaaatccg ggacatctac accggaaaaa tcacgaactg gaagtccgtg
    12301 ggcggccgcg acgcccgcat catcctttat tccagagaaa acaactccgg cacgtacatg
    12361 tttttcatgg agttcgtcct ccgcaataaa gatttcagcc cctacgccat gagcctcccc
    12421 ggaacggccg cggtggcgaa cgccacctct cgcgacaaaa acagcatcgg ttacggcggg
    12481 tcggcctacg ccaaacacat caagttttgc gccgtcaaag ccgacaccga ctcccccgcc
    12541 atcgaaccga cggaagaaaa catcctttcc ggccgctatc ccgtctgccg gaacctcttt
    12601 ttctacactc tgaggaaacc ccggggcccc ctggaacagt tcatctcttg ggtcatgggt
    12661 ccggaaggac aagaaatcgt cgcccagacg ggctatttcc ccgtgaaacg atgaaaccct
    12721 cctccctgtc tcattccgtc tcggaaaaaa taatcgaaac tttcatccac ctgagcgcgg
    12781 ccgtctccat cgtggggttg gtgctgatat tcatcttcat cgggaaagag tccctccccg
    12841 tcttcacctc gcccgagatc cgacgggagg ccagcctgtc gaaaatattt ctgcctcaaa
    12901 gtccggaacc gggagaagaa aaagtttttc gctggcagcc ggtctccgtc gtcccgaaat
    12961 attccctcct cccgctggtg gtcggcaccc tgaaagccac cgcggtggcc cttctcttcg
    13021 ccatcccgct ggccctggcg gccgcgctgt tcacctccga attcgccccc tcgtggctga
    13081 gggagtgggt gaaacccgtc atcgagcttt tggcgggcat cccgtccgtg gtcatgggtt
    13141 ttttcgccct catcgtcatg gccacgtacg ttcaggaact gttcggactg gatttccggc
    13201 tcaacgccat ctccgcgggc atcgccctgg ggctggccgt ggtgccggtg gtctacacca
    13261 tggcggagga cgcgctgtcg gccgttccgc aaagttatcg ggaaggttcg atcgccctgg
    13321 gggccacccc ctggcagacg gcgtggcgcg tggtcttgcc cgccgccttc cccggcattt
    13381 tcgccgcctg catcctggga ctggggcggg ccatcggaga gacgatgatc gtcctgatgg
    13441 cgtcggggaa cgccgccgtg tccagttggt cgcccacgga ccccttccgc agcctctccg
    13501 ccaccatcgc cgccgagatg ggcgaggtcg tccgcggcag cacccactat cacgtccttt
    13561 ttttcatcgg cgtgctgctc ttcctcttca cctttttcat caactatctc ggccaactct
    13621 tcgtggaacg tctccgccag aaactctccg gaacggacaa atgaacctcc cccgtcccgc
    13681 gcgactctca tgaaccgccg ctggatagac ctcttcagcc gcctcttcac gctcctgacc
    13741 gccttggcct gtttctccct ggtggccggc gtggcgttga tcctcggcga cgtcgtcctg
    13801 aacggcctca aacatctgtc gtgggagttc gtctccaccg ccccgaaaac cggcatgacc
    13861 gaaggcggca ttttccccgc cctcttcggg acggtgttcc tggtcctctt gatgacggtg
    13921 gcggtggtcc ccctcggcgt ctccaccgcg atctatcttc aggaatacgc cccggccggt
    13981 tcccgctggg tgaaactgat ccgcatggcc atccagaact tggcgggggt gccggccatc
    14041 gtcttcggac tcttcggact gggcttcttc gttgatttcg tcggcaaagg gatcgaccgg
    14101 ttctttttcg acggggcgct caccttcgga cagcccgcca tcctgtgggc ctcgctcacc
    14161 ttggcgctgt tgaccctgcc gacggtggtg gtggccaccg aggagaccct gcgcgtggtg
    14221 ccgcggagcc atcgcgaagt ggcctattcc ctgggcgcca cacgctggca gatggtccgg
    14281 cgcgtcgtcc ttccgcaggc ggtcaacggc atcctcaccg gcaccatcct ggcggtgagc
    14341 cgcggcgccg gggaggtggc ccccatcctg tttacgggcg cggcctacta tctgccctac
    14401 ctgcccaccc acctcaacca acagttcatg gtcttgaact accacgtgta cgtgatggcc
    14461 acccagtcgc ccgacgtgga cgccaccctg ccgctgctct actccaccgt gctggtcctg
    14521 ctgatgctga cgttcgcgtt gaatttcgcc gccatcatca tccgttcccg cctccggcgg
    14581 cgcctgaaag ccggacgctg acatgacgcc ccctcccgcc ctccaggaaa tgctccgcgt
    14641 cgaggcgctc agcgtgaaaa caaaaaacgc cacgctgctc gaccgcgtca gcctgaacgt
    14701 ccgggaaaga gagatattgt ccatcatcgg ccccgcgggc agcgggaaaa ccacgttcct
    14761 gcggtgtctc aaccggctca tcgacctgga gggaggactg agcgtctccg ggaaagtgct
    14821 cctcggggag caggacgttt tcgacgacgc cgtggacttg gcggcccttc gacggcggat
    14881 cggcatggtg ttcgcgctcc ccacccccct gcccctgtcc atccgcgaca accttcgtct
    14941 gggcctgcgg ttccaaaaat cttcgggccc cggcacgctg gaggaacgca tcgaaacatg
    15001 cctgcgcgcg gcctccctgt gggacgaggt caaggaccgg ctcggcgaat cggcccaaaa
    15061 actctccggc ggccaacagc agcgcctctg cctggcccgg accctgatgg tgcggccgga
    15121 gatcctcctg ttggacgaac cctgctcggg gctggacccc atctccaccg cccagatcga
    15181 ggaggccctc cagtcgctca aggatcagat gcccgtcgtc ctcgtcacca acaacgtcaa
    15241 gcaagcctca cgcacatccg accgcacagc cttctttctc cagggcgaac tggtggagct
    15301 ggacgacacc ggccgccttt tcaccgcccc gcgagacccc cgcacggcgg attacgtctc
    15361 gggacggttc ggatgagacc catggccgcc acctccgtcc atcaagggaa cccgcccgcc
    15421 gcgctcgaaa cccccaagat cgcggtgcaa gacctcaatc tttggtacgg ttcgttccac
    15481 gcccttaaaa acgtccgggt cgatgttctc ccccggaaga tcacggcctt catcggccct
    15541 tcgggctgcg gaaaatccac ggtgctccgt accttcaacc ggatgaacga cctcatcaag
    15601 ggggtgcgcg tcacggggaa agtcctcatt gacgatcaca acatttatga ggaagacacc
    15661 gacctcatcc gtctgcgcaa acgggtgggg atggtcttcc agcggccgaa ccccttcccc
    15721 atctcggttt ttgaaaacgt cgcctacggc ccccgcatcc acggcgaaac tcggcgccga
    15781 cggctggagg aactcgtcga gaaaagtctc cgcgccaccg cgctctggga cgatttgaaa
    15841 gaccgcctct ccacgcccgc gctcggcctg tctcccgaac agcagcaacg cctctgtctc
    15901 tcgcgcctac tggcggtgga gccggacgtc ctcttgatgg acgaaccctg ctcggcgctg
    15961 gaccccatcg ccacggcgcg catcgaggaa ctcgccgtcg agctctccaa ggacttcacc
    16021 atcgtcatcg tcacccacaa catgcagcaa gccgcgcgga tatcccatgt cacggcgttc
    16081 ttcctgctcg gcgaactcct ggaatgcggc gaaacttccc agattttcac caatcccaaa
    16141 aacgagaaaa cggagcagta catcaccggc cgcttcggct gatgtctccc gggaggcaac
    16201 gacatgcaga gacattttga agagaacctg aacgcgctca aggaaaagat catccgcatg
    16261 ggcagtttgg cggaggagat gatccaaaaa tccgtccgcg ccctcatgga acgcaacccg
    16321 tccatcacgc aggacgtctt cgacatggaa agggaggtga acctcctgca tgtggaggtg
    16381 gacgaccgct gtgtgaaact gctggccctc acccagccca tggccgtcga cctgcgcctc
    16441 atcgccgcgg ccatgaagat caacaccgac ctggaacgca tcgccgacca ggccgtcaac
    16501 gtctgccaga cctgctacta ccacctcctg aaagaaacgc ccgtccctca ggccaacatc
    16561 atcccccgca tggccgagat ctcccagtcc atgctgaaga acagcctcga cgccttttcg
    16621 aagaaggaca tcgagctggc gaagaaggtc ctcctgcagg acgaggaaga ggaccggctg
    16681 aaagcggaaa ccttgagcga gctcatcgag ctcatcaagc gcgaaccgga ccattccaaa
    16741 cagttcgtgg acctcatcct catcgccaag aacatggaac gcatctccga ccacgccacc
    16801 aacatcgccg aagacgtcat cttcatggtg ctcggcgtgg acatccgcca tcacatcgag
    16861 gaaaaacggc cgtagggaac agcgggggag gagttcgggc gggaggagat gtctggacgg
    16921 aaaaaccgag ggacggggcc ggcgcgccga gaacacggac ccggcggcct tcgacaacga
    16981 cgttcacttc gcttcaaaca ttaatttttt ggataattcc cggcccccaa ccgccccaga
    17041 accgcgcccc tcctccgccc gcttctgagc ggggaatgac ggcggcgaaa tcgtcgaaaa
    17101 tttatttcaa cgcggcgacc gcggcaagga aacgcggttc gttggcgggg agagagaggt
    17161 cccacttttc cgtctcgatt tcgcgctgca cgagcctggc gcggtccgag gagggcgggt
    17221 gggatttgtt cagggctttc aggcgggtct cgccgctctt ttcagcggcc agccggttca
    17281 gcaggttctg gaaccccgcc ggcgtgtacc cggcggtcgc ggcgtaggag agggccttct
    17341 ggtcggattc acgctcgtcg ccgcgggaga atcccttaaa aagaagcttg atggaaaaat
    17401 cgctgacggc cttgaacgcc tccggactct cctcccgggc gctggccagt tccaccccgc
    17461 cctggatcag gttgcctttc tgtatctcct tggccacgtg ccgccgagcc acatgcgcga
    17521 tctcatgccc caaagcgccg gccaattcgg actcgtcttt cagcccgcgc aagagaccct
    17581 tggtgatgaa gatgtatccc cccggtccgg cgtaggcgtt cacctcgtcg gtattgagga
    17641 tgccgaaatg gtaggggatc tcgggacggt cgcacccttg cgccaccgtg cggccgacga
    17701 ggccgacata tttcgtcaac gccgggtccc gcacaagccc gtagcgtgca cagaggtaag
    17761 aagccacttc cctgcccaac gcgatctcgt ccttgtcgga aaccttcatc atcccctggg
    17821 cgaccttctt ggtcccggaa aaaaccttgt tcaggttgag gccatccacg tccagcgcgc
    17881 ccagaaaggt ttcgccgaac agacacgccg acgccgtcaa aaggaaaaag atttttctca
    17941 tcgcgccttt ttcaacccgc ccgacgactg aaaggtcttg aggtcgtccg gcgaaaccgg
    18001 ctgggcttcc agccacgcca acgcctccgg gtcccgggcg gacaggtccc cttcctcccc
    18061 cggctcgtcc agcccgcgaa ccccggccac gccggtcggc tgggaagcgg aaagtttctt
    18121 gtccgccgac ggccgtaatt tcttgaaatc gaaaccggtg ccggacgggg ggacggccat
    18181 caaccccgcc agcagcacgg cgacggccca ggcccgccgg cgccattttt cagacttcac
    18241 ttccgcgttt ttcatcaaat caacgatacc tcttttagag acggctgaac gcgccgcaac
    18301 gaaaaaagta ttttcctcgt caatgtcgag atggccgacc gggcgatttc ctccaagtcg
    18361 aaccatttgc cgccggccac tttcgggacg tctccacccg cgtcgaacac gaacgagcgt
    18421 acctgcaaat cccgatgaga cagcacatgc cggaacaacg gcaacgcccc caccgggacc
    18481 aaggcgaccc ccaacttctt ctccagcagt tcctcggccg aacggacggc cgttctcgtt
    18541 tcgacgcacg ggaattccca cagaccgccg tagagcccct ccgccggccg ctgcaacatc
    18601 agccatctcc gggccgaacg caccgccccg acgttcaaat ggaccacccg acgtccctgc
    18661 ggttctcccg ccatcggcag cctctccacg gccccgtgcc gatacgccca gcacgttttc
    18721 ctcaacggac acaacaggca ctccggcgag gcgctctggc acaccgtggc gcccaggtcc
    18781 atgatggcgg agttgaacgc atgcgggccc gaaaggcgag cgccggaccg cggcaccagc
    18841 ttccacgcca acccccagag atgttcctgg gtggcggcct tcttcacgtc ctcctcgatc
    18901 ccatagacgc ggctcaagac gcggaccacg ttcccgtcga ccaccggttc cggccggttg
    18961 aaactaaagg acaaaaccgc gccggcggtg tagcggccga tgccgggcag ggagcgcagg
    19021 tcctccgcct tccgcgggac
//
LOCUS       DAOTFD010000027        18322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2034_length_18322_cov_4.187770, whole genome shotgun sequence.
ACCESSION   DAOTFD010000027 DAOTFD010000000
VERSION     DAOTFD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 18322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18322
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_18322_cov_4.187770"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="P5079_03125"
     CDS             <1..960
                     /locus_tag="P5079_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29009.1"
                     /translation="PLFLAVLTGEMLPEATLDYFIGFWTAQGAGSFSSAFFLAERLRS
                     FVKVLGGGYTYLNHWGLPYGQGLVYPGLFLVAVVWILATEVGNFRRRREAGVLPALIV
                     FLAAYLLLSSFAPSMAKELHLLPLLPLAVLITAIGLCGLYGKITHLPLKRLAWGVTCG
                     LALLSSAGGNFSNYDYLRRTGGEFRFQSWAFYDLADWVQARGYRRVVSVAPSLNVKLK
                     FMASSKIVPVDLVLTGRHTDQQIVSRLDRSRGWADAYIAGFWFKGEDRLPTKFQLFSR
                     WRRKQDPPPRLVAVFHERQGRPIYLVYEPLRRPVVKDLKKRTE"
     gene            963..2639
                     /locus_tag="P5079_03130"
     CDS             963..2639
                     /locus_tag="P5079_03130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29010.1"
                     /translation="MLFQAKKLEEKTDMPVGGRGLYRAMVILFMGLYLVLAVWDLESP
                     GMGRDEALMGHSANSIDFVRALKERSLRFLPLQFDNHHGLWMAYAIYPFSKLLGGHPI
                     SIRLAAVFYGFLGLSAMFYVTYRIFGRFVALVTLIFLSCHPSFLCATRIGLHDQNIVH
                     LAAAAAAFFLWRWWTEGKTSQFFFSCFFLGMGMGIQTWFIYWLGALAAVALLNAGEVW
                     KRLKGSWRLHVWGGAASFLAGTGPLFLSFVTGAMRPEDTVDYFITLWLTCGSISLSAA
                     FVMRERLVLFLKMLGGGYTYPNHWGLPYDQGWVYPVLFSVGALWILCAAVRNLAKRRR
                     ETVSTTPLVFFVAYLLCASFAPAWPKEWHLGPLLPVALLVAGIGLCDLFGNIRRLLWR
                     GVAWGVVCVSVFFSSAMGNYVNYDYLHRTGGEFHMYSWAIYDFADWLRTRGHTWVVTM
                     SPYLNVKLRYLASRNIRTVDLGFRIGWTEKEILRRLDSLRSRADVYVATYWAGDEAPF
                     PTRFPMFNRWRMRQDPPLRLVSVFHERQGRPIYLVYEPEKRRRPGRRPPT"
     gene            2731..4242
                     /locus_tag="P5079_03135"
     CDS             2731..4242
                     /locus_tag="P5079_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29011.1"
                     /translation="MSKILLINPAYADVYGASKAGLVRHIIPTLGLATIAADAVRRGH
                     RVKILDMSDSFYDWTKVQRVVREFGPDVVGISATTPLMNQLRDLSVLCRDISKDILLV
                     GGGAHVSALPERSLEESFLDVVVAGEGDIVFGRICDGDSFTDRPGLCRRLERGTVVGA
                     GEGGPLENLDDLPMPAWHLYDPAAYRAKTSRLIVRCPPCLELEFSRGCAYDCHFCASK
                     KIHPLGHRKKSSKRCAEEVKTAERMGWREFVLADDIFTSNREWALSVCEELIRSETSV
                     RWSCLNGIRVDSADLPLFRTMRRAGCYRLAFGFETGNEQRLRASGKGGRASLEAGRRA
                     VRWAREAGMETCGYFLIGLDGDTKETVEDTIAYACSLGLDMLKFGVAMPFPGSALFDA
                     YLARGAIRSFDWDAYNTYANLQPFEHSSLSREEIGFLMGKAYRETVGLNPAFWFRRLR
                     RGLGTGDIFWDPYYFLKFLFSPALNTSRKEAYYARPRWPQPEHAAGRRDRGVH"
     gene            4293..5966
                     /locus_tag="P5079_03140"
     CDS             4293..5966
                     /locus_tag="P5079_03140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29012.1"
                     /translation="MSPSSSHFSRFLHGAALALCLGAFLVLAFWDLKSPGLDRNEAWM
                     GMTAASIDFVQAMKEGSFRSLPLHFDEHHGTWLAYAVYPFLKIMRGSSSALRVSMVFY
                     SFLALIGIYYLARKSYGKTAALFALFLVALHPSYLVITRLGAADGSPLFLLSALMFIF
                     LRRWQQTGRGRDFLLICFFYGLGVGTIFWFVFWGGGLAVVLLLSGREAWNRSGRGLGW
                     HMAWGGVCFLAGMGPLTLACATGNLPLDKVVDYYVDFAHRVAPAGDYSLASVFFWRER
                     WRTFWMTLGGEYTYIRYGASPNVNAWIYNALFVAAVAWSVGRYLGDLKKGARRGFAVD
                     LVLLTVVYVVSATFSPTSLRARHVFPVLPWALFVSAAGFADLCGRLKNILWRRAAWAA
                     AAGLVILSAARCHYANYFYLSRTGGGTSLNSGAVYELAEWVRARGYGRVMVGPPWMRA
                     KLRFLASPTIRTSVSVLAEEPWTPRLAKKCEEHRRETDAYIIPFWNDKEERQSNIFKK
                     FNRWARRQKPAISLVKVLHERNGRPAYLVYEPLDSAKAPLLESAPEGKR"
     gene            5998..7428
                     /locus_tag="P5079_03145"
     CDS             5998..7428
                     /locus_tag="P5079_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29013.1"
                     /translation="MPEKKILLVNPPSRRPTLRDLYCSMTPRGGYNWAPVDLLVLSAL
                     LKERHDLAVLDAVAEGISFEAALVRAAAFRPRGIVSLVGSVTLEDDLGWLQSLKQKTS
                     APVLILGNISLDPDHKVLHDHPWIDGLIQDGAGPELADYFDGDPAGTREYQGLVFRRN
                     GQIRKSPSPARQSTATLAYPPPRHDLFPLHRYRVPWMGAGKTTSMLASYGCVFSCGYC
                     VHRRESIAPRLRPIDDVLRELDSLRDLGVKNIYFRDPIFAGTRENALALTSAMGKRNY
                     PFSWAGSLRIETSSGELLGAMRAAGCRWVAVGIESANADVLEKYRKPLPLEQIKSFIR
                     QCHAAGLLAAGYFILGLPGEDVRSVRETIDFAVASELDFATFTVPVPDYGTTFRKEAE
                     NTETGNGGEPFDRGNPRRGLRTTISEEEYRGLFKEAYRRMYFRPSYVFRMACRLRGVG
                     EWRGAFAHAGRLFWDVWRGGGEVRKR"
     gene            complement(7415..8356)
                     /locus_tag="P5079_03150"
     CDS             complement(7415..8356)
                     /locus_tag="P5079_03150"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRY29014.1"
                     /translation="MGEPAVNTPAIPEHPVEQPAAPLLSRLPPWLVLLRPSHWLKNSF
                     VFAGLIFAHSWRDIPLVLRVAESFAAFCLMASFVYALNDLLDVAVDRTHPKKHRRPLP
                     AGTLSPKAAAGIAALCLLGGLALAAAAGPKILVIVSAYAVLNVAYSLGLKHIVVLDVL
                     LVVAGFMLRLAAGTMGVGIAPSPWLLLCGLLLTLFLALAKRRAELAFQPASARKPLAH
                     YTVDHLDKAIGVVAVGTALAYTLYTVAPGTVTLHKTRSLVFTVPMVMFGLFRYLSLVY
                     RRGRGEDPVTDLLRDGPILAAVLLWTASVIVLLISAS"
     gene            complement(8528..8986)
                     /locus_tag="P5079_03155"
     CDS             complement(8528..8986)
                     /locus_tag="P5079_03155"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRY29015.1"
                     /translation="MNPSKTKILFLCTGNSCRSQMAEGWARHLKGAEIEPYSAGIEIH
                     GMDPRAVEVMTEAGVDIAGQRPKNVRDLEGIAFDYVVTVCGHAQEHCPLFPGKTRVLH
                     VGFNDPPRLAANARSPEEALSHYRRVRDEIKRFVMKLPKALERASVPRQG"
     gene            complement(8983..9675)
                     /locus_tag="P5079_03160"
     CDS             complement(8983..9675)
                     /locus_tag="P5079_03160"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRY29016.1"
                     /translation="MDAFPVSWATALWLGILTSLSPCPLAANVTAVAYIGRLVGHPRH
                     VLWAGLLYTIGRALAYLMLCAVVVFGLLSIPGVSFFLQRRMGQLLGPLLVLTGLFLVF
                     AERFSFSGGGRFSALVQRCVDRGGLWGAAPMGFLFALSLCPVSAALFFGSLIPLAVKH
                     NSIALLPTVYGAGTAIPAVVVAVALAFGGGALGKAYNRLSGIERWARWATAGIFILAG
                     AYMAITTIRNGP"
     gene            complement(9675..10154)
                     /locus_tag="P5079_03165"
     CDS             complement(9675..10154)
                     /locus_tag="P5079_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRY29017.1"
                     /translation="MLPIKSLRGLLWVSLVLGVAASPVSGASPVSRKSPKPSTKNSPA
                     KTEPRKLIVYYFHGNARCMTCRKFETLTKELMDTRFEEEVKQGRVEYRVVNVDDPGNA
                     HFIKDYALYTKSVVLSETSDGEEITWKNLDKIWEKVRNEDAYRRYVEDEVRSYLKRS"
     gene            complement(10142..10558)
                     /locus_tag="P5079_03170"
     CDS             complement(10142..10558)
                     /locus_tag="P5079_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRY29018.1"
                     /translation="MAEKSSCGCGGGTVLIYPCSGAADTGEIADRAARVLSREGIGKM
                     SCLAGIGGRLSGFLASAKGADAVLLIDGCPLDCAKNTMEQAGVTRFQHMRVTDAGLEK
                     GKSPATPERVKRVAEKAKSFLKCRPGGRAGGSSCCP"
     gene            complement(10568..10801)
                     /locus_tag="P5079_03175"
     CDS             complement(10568..10801)
                     /locus_tag="P5079_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY29019.1"
                     /translation="MKKIQILGTGCPKCKKLTELSEAAAKELGIEYSLEKVTDINEIL
                     NFGVMTTPALAVDGTVKTAGRLPSLDEIKNLLR"
     gene            complement(10816..11796)
                     /locus_tag="P5079_03180"
     CDS             complement(10816..11796)
                     /locus_tag="P5079_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY29020.1"
                     /translation="MDPFKPIELFADIATHQWLHFAPSSYAAKTTHFFIYDTIKIGLL
                     LVVINDVMAIVRYYFPLEKTRNILAKRRWYGLDYLFAALLGVVTPFCSCSSIPLFIGF
                     LGAGIPLGVTFSFLISSPLINESSLYLFPALFGLRVTLFYNLFGIAVAVAGGLLIQKL
                     DVGHYVLPDLLKIKSREEVEREHGGGPLSLRRTASLFWREGMAITKQIFPYMVLGVGI
                     GAFIHGFVPRGFVERYFSAKTWWSIPLVTLLGAPFYANSVSVLPVMEALVGKGVPLGT
                     ALTFMTSIVTISIPEALILKKVMRWPLLGLFFGITIIGIMAMGFLFNAIL"
     gene            complement(11813..12628)
                     /locus_tag="P5079_03185"
     CDS             complement(11813..12628)
                     /locus_tag="P5079_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRY29021.1"
                     /translation="MDETRTKVREAYGKIARQGGSCCGTSSCCGGPSADDVAEAVGYD
                     DGERAGLPDGANMGLSCGNPTAVASLRPGEVVLDLGAGGGFDVFIAARKVGPTGRVIG
                     VDMTPEMVAKARRNAVSFGRSTGLSNAEFRLGEIEHLPVEDSSVDVVISNCVINLSTD
                     KARVWREVARVLKPGGRVAVSDLALRRPLPESVRSSVQALVGCIAGAVLLDETKAMVQ
                     AAGLKNVVLETKSDYIDKMSEWSDPLYRDIADKLPPGTKPSDFVTSLNLTAKK"
     gene            complement(12621..13679)
                     /gene="arsB"
                     /locus_tag="P5079_03190"
     CDS             complement(12621..13679)
                     /gene="arsB"
                     /locus_tag="P5079_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRY29022.1"
                     /translation="MKKLSFLDRYLTLWIFLGMGAGVFLGYFVSGIERFINLFQIGTT
                     NIPIAAGLILMMYPPLAKVKYEEMGDVFRNWKVLGLSLVQNWVVGPVLMFVLAIVFLR
                     NYPEYMVGLIMIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSVFQVLFYSAYAYVFI
                     TVLPPLFGLKGSMVQVSMGQIAESVFIYLGIPFIAGVATRIVGLKIGGKEWYEKTFIP
                     KISPITLTALLFTIVVMFSLKGEMIVRIPLDVVRIAIPLLIYFVVMFLVSFWMGKKVG
                     ADYRKSATLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLVEVPALIGLVNVSL
                     YFQKKYFHTSIEGQNRHG"
     gene            complement(13756..14127)
                     /locus_tag="P5079_03195"
     CDS             complement(13756..14127)
                     /locus_tag="P5079_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY29023.1"
                     /translation="MVHIRKDGAAARLSAMADPLRLRILRLLLERELCVCEIVRTLEQ
                     PQYKISRHLTVMKRAGLVNDWREGTWAHYEINSSLSDHWRRALAVLGRAWEESPLVQK
                     DLRRLRGAAGRKPGESARSRA"
     assembly_gap    14395..14404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14405..15855
                     /locus_tag="P5079_03200"
     CDS             <14405..15855
                     /locus_tag="P5079_03200"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRY29024.1"
                     /translation="PVHMNPSKAAAAPVSPAALNLQDSFANVAAKAKPAVVNIISEHV
                     ESVEAAPYEFFFGDPFEFFFQDSPYDPPRRRAPSRRFERRQQGMGSGVIIDARGYILT
                     NEHVVRDAQSIQVRLPEEGDRVYKGKVVGTDPRSDLAVIRIKAKGDLPYAALGDSDKV
                     RVGDWSIAIGSPFGLEQTVTVGVISAVRQSLNIEGKNYQNMLQTDAAINQGNSGGPLL
                     NIRGEVIGINTAIYAPTGVFSGVGFAIPSGYAREIMEQLIETGRVVRGWLGVEIAPLD
                     DVLAKQFGLKDTEGVLLNRVLPDSPASKAGLKRGDVIRLFDGQKVASPESLQRLVAKT
                     PPEKKVELKVWREGKESTISLVTGKMPESTAEGEAGPSNSSDGAGKEKQSESFDWEGG
                     HFVPNSAAWAKRYDLPEDAPGVVLARLEPGSLVEAVGLTPGDLIASVNREATPDLDRF
                     KKALKKVDFKSGAMFDIYRQGRWFYLSYRKEE"
     gene            complement(15885..16238)
                     /locus_tag="P5079_03205"
     CDS             complement(15885..16238)
                     /locus_tag="P5079_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRY29025.1"
                     /translation="MSLIAHLFVNTLAVLTGAYLLRGVHVDKLLTAVVVAIVLGLVNV
                     VIKPLLVLITLPISILSLGLFLFVINAALIMLVAWWVPGFSVDSFWWALAYSLVLTVV
                     SAFLNSLKKMGNRTP"
     gene            16339..17448
                     /locus_tag="P5079_03210"
     CDS             16339..17448
                     /locus_tag="P5079_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRY29026.1"
                     /translation="MKTSPFRLTGRGADTNPRNRFSSTHSVPEPEDAAPPDLPPKTRF
                     LRDTSRRVISCHHSPDLGYGATLNPYRGCEHGCVYCFARPTHEYLDRSAGLDFESVVF
                     IKHDAARLLRRELGAPRWEPRTVAMCGVTDPYQPIERRRELTRECLKVFLEFRNPVTV
                     TTKNQLVTRDRDILSALAGWNAAAVFISVTTLDEELCRRMEPRTSRPAHRLEAVKALA
                     QAGIPVGVNVAPVIPGLTDHELPAILKEAALAGAKYAWYAPVRLPYGVGELFERWIEK
                     YFPDRKNKILGRLRSLHGGKLNDSSFGARLRGRGVYADELEGLFRLSCRRLGLRDDGP
                     ALSAASFRRPVGRQLALFSGSASDSGCPGDVEMIK"
     gene            17589..>18322
                     /locus_tag="P5079_03215"
     CDS             17589..>18322
                     /locus_tag="P5079_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY29027.1"
                     /translation="MVVVGGGFAGAAAASALTESGVTVTLLERRPTLGGRTSSFRDGV
                     TREEADNGQHLFMGCYKEVRRFLRRLKVGDRVQFFPSLDVKFLSPAGAAELACPVLAG
                     HLGVLWGIGRYGAMSVGDRFSVLRALSGLRLRKRWGDLKNVTVSQWLAALRQPAGARR
                     AFWTPLCLATLNERPEKACAEALAVVLREGFFSDSSSSAVGYCTVALGKLWSVELFAY
                     LKRQGGVVASRQAARGFEAQDDLVRSV"
BASE COUNT         3388 a         5240 c         5886 g         3798 t
ORIGIN      
        1 cccttgttcc tggcggtgct gacgggggag atgttgccgg aggccacgct ggattatttc
       61 atcggttttt ggacggctca gggggccggt tctttttctt ccgctttttt cctcgcggag
      121 aggcttcggt cgttcgtcaa ggttttgggg ggaggataca cttatttgaa tcattggggg
      181 cttccgtatg gtcaggggtt ggtttatccc ggtttattcc tcgtcgccgt cgtctggatc
      241 cttgccacgg aggtggggaa ttttagacga cggagggaag cgggggtcct gccggcgctg
      301 atcgtttttc ttgccgccta tctgctcctc tcgagttttg cgccgtcgat ggcgaaggaa
      361 ttgcatcttc tcccgcttct gccgctggcc gttttgatca ccgccatcgg cttgtgcggg
      421 ctgtacggga aaatcacgca tcttccactg aagcgtttgg cttggggggt gacgtgcggc
      481 ctggcgctgc tttcatcggc cggcggaaat ttttccaatt acgactacct ccgccggacg
      541 ggtggcgagt tccggtttca gtcctgggct ttttacgatc tggccgattg ggttcaggcg
      601 cggggatacc ggcgcgtggt gtcggtcgcg cccagtctga acgtcaaatt gaaattcatg
      661 gcttcttcga aaatcgttcc cgtggacttg gttcttacgg gacgacacac cgaccagcag
      721 attgtgagcc ggctcgatcg ctcgcggggt tgggcggatg cctacatcgc cggtttttgg
      781 ttcaagggag aggatcgtct tccgacgaaa tttcaattgt tcagtcgctg gcgcaggaaa
      841 caggacccgc ctccgcggct ggtggcggtg ttccacgagc gccagggccg cccgatttat
      901 ctcgtgtatg aaccgcttcg tcggccggta gtcaaagacc tgaagaaaag aacggaatag
      961 gcgtgctgtt tcaggcgaaa aaactggaag aaaagacgga catgcctgtc ggaggccggg
     1021 gcctctaccg tgccatggtg atccttttca tgggcctcta cctcgtcctg gctgtttggg
     1081 atttggagag tcccggcatg ggaagggatg aggcgctgat ggggcattcg gcaaactcca
     1141 ttgattttgt ccgggcgctt aaagaaagat cgttgcggtt tcttcccctg cagttcgaca
     1201 accatcacgg tctgtggatg gcttacgcca tttatccttt ttcgaaattg ctgggaggtc
     1261 accccatctc gatccgcctg gcggccgtgt tctatgggtt tctggggctg tcggccatgt
     1321 tttatgtgac gtatcggata tttggccgct tcgtggcctt ggtgacgctg atttttttat
     1381 cttgtcaccc gtcgtttcta tgtgcgaccc gcatcggctt gcatgaccaa aatatcgtgc
     1441 atttggctgc tgccgcggcg gcttttttct tgtggcgatg gtggacggag ggaaagacga
     1501 gccaattttt tttctcgtgt tttttcctcg gcatgggaat ggggatacag acgtggttca
     1561 tctattggtt gggggccctg gccgcggtgg cgctgttgaa cgccggagag gtttggaagc
     1621 gactgaaggg gagctggcgt cttcatgtgt ggggaggggc ggcaagcttt ctggccggga
     1681 cggggcccct tttcctttcc ttcgtcacgg gggccatgcg ccccgaggac accgtcgatt
     1741 atttcattac gttgtggctg acgtgcggct ccatttccct ctccgccgct tttgtcatgc
     1801 gggagaggct cgttcttttt ttgaaaatgt tggggggcgg atatacctac cccaaccatt
     1861 ggggtttgcc ttacgaccag ggttgggttt atcccgtcct cttttcggtg ggcgcattat
     1921 ggatcctatg tgcggccgtt cggaatctgg ccaaaaggcg aagggaaacg gtctcgacaa
     1981 cgccccttgt tttcttcgtc gcctatttgc tttgcgcaag tttcgcgccg gcttggccga
     2041 aggagtggca tctcggtccg cttttgccgg tggcgctttt ggtcgccggc atcggattgt
     2101 gcgatctttt cgggaatatc cgccgattgc tctggagggg ggtggcttgg ggggtggtgt
     2161 gtgtctcggt gttcttttct tcggccatgg gcaattatgt caactatgat taccttcacc
     2221 ggaccggcgg ggaatttcat atgtattcct gggccattta cgatttcgct gattggctca
     2281 ggacgcgcgg gcatacgtgg gtggtgacga tgtcccccta tcttaatgtc aaattgagat
     2341 acctggcatc gaggaacatc cgcaccgttg atctggggtt tcggatcggt tggacagaga
     2401 aggaaatttt acggcggctc gacagcctgc ggtcgcgcgc ggatgtctat gtggcgacgt
     2461 attgggccgg agacgaggcc ccgtttccca cccggtttcc catgttcaac cgttggcgca
     2521 tgagacaaga cccgcccctg cggctggtgt cggtgttcca cgagcgccag ggccgtccga
     2581 tctatctcgt ttacgaaccg gagaaaaggc ggcgaccggg ccgtcgcccg ccaacgtgag
     2641 tcgtcggtcg gccgtccctt attcgggccc ggttgttttt gggggggcgt gtccccggag
     2701 acgtttcgtc cggcccgaaa aagcgaattt atgtcgaaaa tccttctgat caatccggcc
     2761 tatgccgacg tctatggggc ttccaaggcg ggccttgtga ggcacatcat ccccaccctg
     2821 ggactcgcga cgattgcggc cgacgccgtc cgtcgggggc acagggtcaa gatattggac
     2881 atgagtgatt ctttttatga ttggacgaaa gtccagcggg tcgttcggga gttcggcccg
     2941 gacgtggtgg ggatttcggc caccacaccc ctcatgaacc agcttcggga tctgtcggtc
     3001 ctgtgccggg acatttcgaa ggacattctt ttggtcggcg gcggggcgca tgtttccgct
     3061 ctcccggagc ggagcctgga ggaatctttt ttggacgtcg ttgtggcggg ggagggggac
     3121 atcgttttcg gccggatatg cgacggtgac tcctttacgg atcggccggg gctttgtcgg
     3181 cggctcgaac gtgggacggt ggtcggggcc ggggaggggg ggccgctgga gaatttggat
     3241 gacctgccca tgccggcgtg gcatttgtac gatcccgccg cctatcgggc gaagacaagc
     3301 cggctcatcg tccggtgtcc tccctgcctg gaactggaat tttccagggg ctgcgcgtac
     3361 gattgccatt tctgcgcctc aaaaaaaatt catccgctcg gccacagaaa aaaatcatcc
     3421 aaacggtgcg cggaagaagt gaaaacggcc gaaaggatgg ggtggcggga gttcgtcctg
     3481 gccgacgata ttttcacgtc gaaccgggaa tgggcgttga gcgtctgcga agaattgata
     3541 cgatcggaaa cgtcggtccg ctggtcctgt ttgaacggca tccgggtgga ttcggccgac
     3601 ctgccgcttt tccggacaat gcgccgcgcc ggatgttacc ggctcgcctt tgggtttgag
     3661 acgggcaacg aacagaggct gcgggcgtcg ggaaagggcg gtcgggcttc cctggaagcg
     3721 ggccggcggg ccgtccgttg ggcccgcgag gccgggatgg agacgtgcgg gtattttctt
     3781 atcggactgg acggcgatac gaaggagacg gtggaagaca ccatcgcgta tgcgtgttct
     3841 ctgggactcg acatgctcaa gttcggcgtg gccatgcctt tcccggggtc ggctcttttt
     3901 gacgcttatc tggcccgcgg cgcgatccgt tcttttgatt gggatgcgta taacacgtac
     3961 gccaacctcc agccctttga acattcctcg ttgagccggg aagagatcgg ttttctcatg
     4021 ggaaaggcct accgggaaac ggtcggcttg aacccggctt tctggttccg ccgtttgagg
     4081 cgcgggctgg gaacggggga tatcttttgg gacccgtatt attttttaaa attcctattt
     4141 tccccggcgc tcaacacgtc gaggaaagag gcgtactacg cccggccccg ttggccgcag
     4201 ccggaacatg cggcggggcg tagggatcgg ggagttcatt gatgggagag ccgggaactt
     4261 cggggtcgga cgccgatcgg agcggagaag tcttgtctcc ctcgtcgtcc cacttttccc
     4321 gatttctgca cggagccgcg ttggcgctgt gcctcggcgc gttcctggtt ttggcttttt
     4381 gggacttgaa aagtcccggg ttggaccgga acgaggcgtg gatggggatg acggcggcgt
     4441 ccatcgactt cgttcaggcg atgaaggagg gttccttccg gtctcttccc ctgcatttcg
     4501 atgagcatca cgggacctgg ctcgcctacg ccgtctaccc gtttttgaaa atcatgaggg
     4561 gctcgtcgtc ggccttgcgc gtttccatgg tgttctacag tttcctggcg ttgatcggca
     4621 tctactattt ggccagaaaa tcgtacggaa agacagccgc cctcttcgcc ctgttcctcg
     4681 tcgcactcca tccgtcctat cttgtcatca cccgtttggg agccgcggac ggcagcccgc
     4741 tgttcctgct ctcggcgttg atgttcatct tccttcggcg ttggcaacag acggggcggg
     4801 gacgggattt tttgttgatc tgtttttttt acgggttggg cgtggggacg atattctggt
     4861 tcgttttttg gggcggcggt ttggcggtcg tcttgctttt gtccgggcgg gaggcctgga
     4921 accgctccgg ccgcggtctg ggatggcata tggcctgggg aggcgtgtgt ttcttggcgg
     4981 ggatgggccc cttgacgctg gcgtgcgcca cggggaatct cccgctcgat aaagtcgtcg
     5041 attactatgt ggattttgcg catcgtgtcg ctccggccgg ggactactcg ctggcgtccg
     5101 tttttttctg gagggaacgg tggcgcacct tctggatgac gttgggcggc gaatacacgt
     5161 acatccgcta tggggcctct ccgaacgtca acgcatggat ttacaacgca ttgtttgtcg
     5221 ccgctgtggc ttggtccgtc ggccgctatc tcggggatct taaaaagggg gcaaggaggg
     5281 gctttgcggt cgatctcgtc ctgttgacgg tcgtctatgt cgtgtccgcg acattttcgc
     5341 ccaccagctt gcgggcgcgg cacgtttttc cggttctccc gtgggcgctg ttcgtttccg
     5401 ccgccgggtt cgccgacctc tgcgggcggc ttaaaaatat tttgtggcgg cgggcggcct
     5461 gggcggcggc ggcgggcctg gtgatccttt cggcggcgag atgccattac gcgaactact
     5521 tctatctttc caggaccggc ggggggacga gcctgaactc gggggccgtc tatgaattgg
     5581 cggaatgggt gcgtgcgcgt ggatacggac gcgtgatggt ggggccgcct tggatgcgcg
     5641 ccaaacttcg ttttttggct tcgccgacaa tacgcacgtc ggtttctgtg ttggcggagg
     5701 aaccctggac gcctcggctt gcgaagaagt gcgaggaaca tcgccgggag acagacgcct
     5761 acatcatccc tttctggaac gacaaagagg agcgtcagtc caacattttc aagaagttca
     5821 accggtgggc gcgtcggcag aaaccggcca tttccctggt gaaggttctg catgagcgga
     5881 acggccggcc cgcctatctt gtgtatgagc cgttggactc ggcaaaggcc cctctcctcg
     5941 agagcgcgcc ggagggaaaa cgatgagacg cggccgacgg cgggcgtaga ggatccggtg
     6001 cctgaaaaaa aaatcctttt ggtgaatccc ccgtctcgac ggcccacctt gcgcgatttg
     6061 tattgttcga tgacgccgcg gggaggttac aactgggcgc ccgtggatct tttggtcctc
     6121 agcgctcttt tgaaagagcg gcatgatctg gcggtgttgg acgccgtggc cgaggggatt
     6181 tcgtttgagg ccgccctggt ccgcgccgcg gcgttccgcc cccgggggat cgtttccctc
     6241 gtcggttccg tcacgttgga agacgacctg ggctggctgc aatcactgaa acagaaaacg
     6301 tcggccccgg tccttatcct ggggaacatt tcactggacc cggaccacaa ggtgttgcac
     6361 gaccatccgt ggatcgacgg cctgattcaa gacggcgccg gtcccgagct ggccgactat
     6421 tttgacgggg acccggccgg cacgagggaa taccaaggtc ttgtttttcg gcggaacgga
     6481 caaatccgga agagcccctc tcccgcgaga caatcgacgg caacgctcgc ctatcccccg
     6541 ccgcggcacg acttgttccc gctgcaccgc taccgcgtcc catggatggg ggcgggaaag
     6601 acgacgtcca tgttggcaag ttacggttgt gttttctcct gcggttattg cgtgcacaga
     6661 cgggaaagca tcgccccgcg gttgcggccg atcgacgacg tgttgcggga actggattcc
     6721 ctgcgggacc tcggagtgaa gaacatttat ttcagggatc cgattttcgc cggcacccgg
     6781 gagaacgccc tggcgctgac gtcggccatg ggaaagagga actacccgtt ttcatgggcg
     6841 ggcagtttgc gcatcgaaac gtccagcggg gagttgttgg gggcgatgcg ggcggccgga
     6901 tgccgatggg tggcggtcgg catcgaatcg gccaatgccg acgtgttgga gaagtatcgg
     6961 aagcccctcc cgttggagca gatcaaaagt ttcatccgtc aatgccatgc ggccggactt
     7021 ctggcggcgg gctatttcat cctgggcctg cccggcgagg acgtgcggtc cgttcgggag
     7081 accatcgatt tcgccgtcgc gtcggagctg gatttcgcca cgttcaccgt ccccgtgccg
     7141 gattacggca cgacttttcg gaaagaagcc gaaaatacgg agaccgggaa cgggggggaa
     7201 ccgtttgacc ggggaaatcc gcgccgcggc ttgaggacga ccatttcgga agaagagtat
     7261 cggggccttt tcaaggaggc ctaccgtcgc atgtatttca ggccgtccta tgtgttccga
     7321 atggcgtgcc ggcttcgggg ggtgggagag tggcgcggtg cgtttgccca cgccggtcgg
     7381 cttttttggg atgtgtggcg gggaggaggg gaggttagga agcgctgatc agcaggacga
     7441 tgaccgacgc cgtccaaagg agcaccgccg ccaggatggg accgtctcga agcaggtcgg
     7501 tcaccggatc ctcgccgcgg ccgcggcgat acaccagaga aagataacgg aacagaccga
     7561 acatcaccat cggcacggta aaaaccagag accgggtctt gtgaagggtc accgtgcccg
     7621 gcgccacggt gtacagggta taggccagcg cggtcccgac ggcgacgacc ccgatcgcct
     7681 tgtccaggtg atcgacggtg taatgggcca gcggttttcg agcggaggcc ggctgaaaag
     7741 ccagttcggc ccgccgtttg gccagcgcaa ggaagagcgt gagcaatagg ccgcagagga
     7801 ggagccaagg ggagggggcg atgcccaccc ccatcgtgcc ggcggccagc cgcaacatga
     7861 atcccgcgac gacgaggagc acatccaaga cgacgatgtg cttgagcccg agggaataag
     7921 ccacgtttaa gacggcgtac gccgagacga tgacaaggat tttgggcccg gccgcggccg
     7981 ccaacgccaa cccgccgagc agacagaggg ccgcgatgcc cgccgccgct ttgggcgaca
     8041 gggtccctgc cggcaagggg cggcgatgtt ttttcggatg cgtccgatcc accgccacat
     8101 ccagcaggtc gttcagcgca taaacgaaac tggccataag acagaacgcc gcaaaggatt
     8161 ccgccacccg gagcaccagg gggatgtccc gccaggagtg cgcgaagatc aggccggcga
     8221 aaacaaaact gtttttgagc cagtgagagg gccgaaggag gacgagccag ggcgggagac
     8281 gggacaggag aggcgcggcg ggttgttcca cggggtgctc ggggatggcg ggcgtattca
     8341 ccgcgggttc ccccatggga ttttttggat tgggatacag acgtgttttt tccggcggca
     8401 tggtggcgat tttatagcaa attatgacgg gtttccggaa cgccggccga gggcgctccg
     8461 agggcggatt ccatgcggcc ggccgcggtc tttccgtccg cttctttcat cggcgccttg
     8521 atcttatcta tccctgacgc gggacactcg cgcgctccaa ggccttcgga agcttcatga
     8581 cgaatctttt gatctcgtcg cgcactcgcc gatagtgaga gagggcctct tccggcgacc
     8641 gggcgttcgc cgccagccgg ggagggtcgt taaatccaac gtgaagcact cgggttttcc
     8701 cggggaaaag gggacagtgt tcctgggcgt gcccgcagac cgtcaccacg taatcgaacg
     8761 cgatcccttc caggtcccgc acgttcttgg gccgctgtcc ggcgatatcc accccggctt
     8821 ccgtcatgac ctccaccgcc cggggatcca tcccgtgaat ttcaatgccg gcggaatacg
     8881 gttcgatctc ggcgcccttt aaatggcgcg cccacccttc cgccatctgg ctccggcagg
     8941 aattgcccgt gcagaggaaa aggatcttgg ttttcgacgg gttcatggac cgtttcggat
     9001 ggtcgtgatg gccatataag cgccggccag gatgaagatt cccgcggtgg cccatcgcgc
     9061 ccaacgctcg atgccgctga gtcggttgta ggcctttccc agcgccccgc cgccaaaggc
     9121 cagggccacc gccacgacga cggccgggat ggccgttccc gcgccataaa cggtcggaag
     9181 aagtgcgatg gagttatgct tcacagccaa gggaatcaaa ctcccgaaaa aaagagccgc
     9241 cgacacgggg cataatgaca acgcgaacaa aaagcccatg ggggccgcgc cccacaggcc
     9301 gccgcggtcg acacaccgtt gaacaagagc gctgaatcgg ccgccgcccg agaaagaaaa
     9361 gcgttccgca aagaccaaaa aaagaccggt cagaacgaga agcggcccca gaagttggcc
     9421 catgcgtctt tgcaggaaga acgaaacgcc tgggatggag agaaggccaa aaaccacgac
     9481 cgcgcagagc atcagatagg cgagcgcgcg tccgatggta taaagaagcc cggcccacag
     9541 gacatggcgg gggtgcccca cgagccttcc tatataggcc accgccgtta cgttggcggc
     9601 cagggggcag gggctcaacg aagtcaggat ccccagccac agggccgtcg cccaacttac
     9661 agggaaagca tccatcaact tcttttcaga taggaacgca cttcgtcctc cacataccgc
     9721 cggtaggcgt cttcgttccg caccttctcc cagatcttgt ccaggttttt ccaggtgatt
     9781 tcttccccgt cgctggtttc ggagagcacg acggatttcg tgtaaagagc gtagtccttg
     9841 atgaaatgcg cgttcccggg gtcgtccacg ttcaccacgc ggtattccac ccgcccctgt
     9901 ttgacttctt cctcgaatcg cgtgtccatg agttctttcg tcagggtttc gaatttgcga
     9961 caggtcatgc accgggcgtt cccgtggaaa taataaacga tcagtttccg cggttcagtc
    10021 tttgccgggc tgtttttcgt tgagggtttc ggggatttcc gtgacaccgg actcgcccct
    10081 gaaacggggg aggccgccac tcccagaacg agagaaaccc aaaggagccc ccggagtgat
    10141 tttatgggca acatgaggaa cctcccgcgc ggccgccggg gcggcacttg agaaaagatt
    10201 tcgctttttc ggcgacccgc ttcacgcgct ccggcgtcgc cggggatttc cctttttcaa
    10261 gccccgcgtc ggtcacgcgc atgtgctgga accgcgtcac gccggcctgc tccatcgtgt
    10321 tcttggcgca gtccaacgga caaccgtcga tgagcaaaac ggcgtccgcg cctttcgccg
    10381 aagccagaaa accgctcagc cgtcctccaa tgcccgccaa gcaggacatt ttcccgatcc
    10441 cttcgcggga caagacccgg gcggccctgt cggcgatctc tccggtgtcc gccgccccgg
    10501 aacaggggta gatcagcacc gtgcccccgc cgcacccaca agaagatttt tctgccatga
    10561 gcgtctccta tctcaacagg ttttttattt catcaaggga cggcaggcgc cccgccgttt
    10621 tgaccgtccc atcgaccgcc agcgcgggcg tcgtcatcac gccgaaattc aggatctcgt
    10681 tgatgtcggt cactttttcc agggaatatt caatgcccaa ttccttggcg gccgcctcgg
    10741 aaagctccgt cagcttcttg catttgggac aacccgttcc caggatttgg atttttttca
    10801 tgggtcctcc gtcgctcaca ggatcgcgtt gaacaaaaag cccatcgcca tgatgccgat
    10861 gatcgttatg ccgaaaaaga gccccaagag cggccaccgc atcactttct tgagaatcag
    10921 cgcttccggg atggaaatcg tgacgatgga ggtcatgaaa gtgagcgcgg tcccgagggg
    10981 cacgcctttg cccaccaggg cctccatgac cggaagaacg ctgaccgaat tggcgtagaa
    11041 gggcgcgccg agaagggtca ccagggggat gctccaccac gttttcgccg agaaatatcg
    11101 ttcaacgaag ccccggggta caaaaccgtg gatgaaagcc ccgatgccga cccccagcac
    11161 catgtaaggg aaaatctgtt tggtgatggc catcccctcc cgccaaaaga gggaagccgt
    11221 ccgtcttaaa gacaaggggc cgccgccgtg ttcccgttcg acctcttccc gggacttgat
    11281 cttcaacagg tccggcaaca cgtaatgtcc aacgtcgagc ttctggatca gcaggccgcc
    11341 cgccaccgcc acggcgatgc cgaacaggtt gtagaacagg gtgaccctga ggccgaacag
    11401 ggcgggaaaa aggtagagcg acgattcgtt gatgaggggc gaactgatca ggaaagagaa
    11461 cgtcactccc aaggggatcc ccgcccccag aaatccgatg aaaaggggaa tggacgagca
    11521 ggaacagaac ggcgtcacca cgcccagaag cgccgcgaac aggtaatcca agccatacca
    11581 gcgccttttg gcgaggatgt tccgcgtctt ttccagggga aagtaatacc ggacgatcgc
    11641 catgacgtcg ttgatgacga cgagcagaag gccgatctta atcgtgtcat agatgaaaaa
    11701 atgcgtcgtc ttcgccgcgt aggaagaagg cgcaaaatga agccactggt gggtggcgat
    11761 atccgcgaac agctcgatgg gtttgaatgg atccatggac ggtccggcgc ccttattttt
    11821 tggccgtcag gttgaggctg gtcacgaaat cgctcggttt tgtccccggc ggtagtttgt
    11881 cggcgatatc ccggtagaga gggtccgacc attcgctcat cttgtcgatg taatccgatt
    11941 tggtttcgag aacgacgttt ttgaggccgg cggcctggac catcgccttc gtctcatcga
    12001 ggaggaccgc gccggcgatg catcccacaa gggcctgcac ggaggaacgg acgctttcgg
    12061 gcagcgggcg ccggagggcc aggtcggaga cggcgacccg tccgcccggc ttgagcacgc
    12121 gcgccacttc ccgccacacg cgggctttgt ccgtggagag gttgatgacg cagttggaga
    12181 tcaccacgtc cacgctggaa tcctccacgg gcagatgctc gatctcgccc aagcggaact
    12241 cggcgttgga gagcccggtc gatcggccga aagaaaccgc gttccggcgc gctttggcga
    12301 ccatctccgg cgtcatgtcg acgccgatca cgcgccccgt cggccccacc ttccgcgccg
    12361 cgatgaacac gtcgaacccg ccgcccgccc ccaggtcgag gacgacctcg cccggccgca
    12421 gggaggccac cgcggtgggg tttccgcagg aaaggcccat gttcgccccg tcggggaggc
    12481 cggcccgttc gccgtcgtcg taacccacgg cctccgcgac gtcgtcggcg gaaggcccgc
    12541 cgcaacagga cgacgtcccg caacaggaac cgccctgtcg ggcgatcttg ccgtaggctt
    12601 cccgcacctt cgtccgtgtt tcatccatga cggttctgcc cctcgatcga tgtgtggaaa
    12661 tactttttct ggaaatagag ggagacgttc accagcccga tgagggcggg cacctccacc
    12721 agggggccga tcaccgccgc gaaagccgcc ccggagttga tgccgaacac cgccaccgcc
    12781 accgcgatgg ccagctcgaa attgttgctg gccgccgtga aggacagcgt ggccgatttc
    12841 cggtagtccg ccccgacttt tttgcccatc cagaagctca ccaggaacat caccacgaaa
    12901 tagatgagca gggggatcgc gatccggaca acgtccaacg ggatgcggac gatcatttca
    12961 cctttcaggc tgaacatgac gacgatcgtg aaaaggaggg cggtcagcgt gatcgggctg
    13021 attttgggga tgaacgtctt ctcataccat tccttccccc cgatcttgag cccgacgatc
    13081 cgggtcgcaa cgcccgcgat gaagggaatc cccaggtaaa taaagacgct ttcggcgatc
    13141 tgccccatgc tcacctgcac catgctgcct ttcagcccga acaggggcgg caacaccgtg
    13201 atgaagacat aggcgtaggc gctgtaaaaa aggacctgga acacgctgtt gaaagccacc
    13261 agcccggcgg catattccgt gtcccccttg gccaactcgt tccatacgat gaccatggcg
    13321 atgcaccgcg ccaaaccgat catgatgagg cccaccatgt attccgggta gtttcgaaga
    13381 aacacaatcg ccaaaacgaa catcaacacc gggccgacga cccagttttg caccagggag
    13441 aggcccagca ctttccaatt gcggaacacg tcgcccattt cctcgtattt caccttggcc
    13501 aggggcgggt acatcatcag gatgagcccc gccgcgatgg ggatgttggt cgtgccgatc
    13561 tggaacagat tgatgaaccg ttcgatgccg gacacgaaat agcccaggaa gacgcccgcg
    13621 cccatgccca ggaatatcca cagggtcagg tatcggtcca gaaaggaaag tttcttcaca
    13681 acgctttcag aaggattcac ggaggcctcc gaggatatat ttatgcgctg tatcgcatat
    13741 agaaggacaa aaaaatcagg cgcggcttcg cgcgctttcg ccgggtttgc ggcccgccgc
    13801 cccgcgcagg cggcggaggt ctttttgaac gaggggggat tcctcccatg cccggcccag
    13861 gaccgccagg gcccggcgcc agtggtccga caacgaggag ttgatctcat agtgcgccca
    13921 ggtgccttcc cgccagtcgt tcaccaggcc ggcgcgtttc atcaccgtga ggtggcgcga
    13981 aattttatat tgcggttgtt ccagcgtccg cacgatctcg cagacacaga gctcccgctc
    14041 cagaaggagg cggaggatcc ggagccggag agggtcggcc atcgcggaga ggcgggcggc
    14101 cgccccgtcc tttcttatat gcaccatatc gcatattata gcacgcgcga tccggcgatg
    14161 tcaatttttt gagcgacgag tggtctgccc gcgggcatct taggtaaaat atgtcggacg
    14221 ggcgcgtttc cggccccaca gttcagacag gaaggagtcg gccatgaatt tgaacacgaa
    14281 gaataaggaa aaaccgggga tgtggaaagg gatccttgtc tttctggttt tggcggtcgc
    14341 cgtgttgtgg gtgttccggg cggacagccg ggatttcacc tccttctttt ctttnnnnnn
    14401 nnnngccccg tgcacatgaa cccatccaaa gccgctgccg cgccggtgtc tccggccgcg
    14461 ctcaatctgc aggattcctt cgccaacgtg gcggcgaagg cgaaacccgc ggtggtgaac
    14521 atcatctcgg aacacgtgga gtccgtggag gcggcgcctt acgaattctt cttcggggac
    14581 ccgttcgagt tctttttcca agacagcccc tacgacccgc cccgtcgccg cgcgccgtcc
    14641 cgccgctttg agcgccgcca gcagggcatg gggtccggcg tgatcattga cgcccgcggg
    14701 tacattctga ccaacgaaca cgtggtgcgc gacgcccaaa gcatccaggt gcggctcccg
    14761 gaggagggcg acagggttta taaaggaaag gtggtcggta ccgatccgcg ttccgacctg
    14821 gcggtgatcc ggatcaaggc caagggggat ctgccgtacg cggccctggg cgattccgac
    14881 aaggtgcggg tgggcgattg gtccatcgcc atcggcagtc cgttcgggtt ggagcagacg
    14941 gtgaccgtgg gcgtgatctc ggcggtgcgc caatccctga acatcgaagg gaagaattac
    15001 cagaacatgc tgcagacgga cgccgccatc aaccaaggca actcgggcgg gccgttgctc
    15061 aacatccggg gagaggtgat cggcatcaac acggccatct acgcccccac cggggtgttc
    15121 tccggggtcg gtttcgccat ccccagcggc tacgcccggg agatcatgga acaactgatc
    15181 gagacgggcc gcgtggtgcg gggatggttg ggcgtggaga tcgcgccgtt ggacgatgtg
    15241 ttggccaagc agttcggttt gaaagacacg gagggggttt tgctcaaccg ggttctgccg
    15301 gactctccgg cgtcgaaggc cggactgaaa aggggcgacg tgatccgcct gttcgacgga
    15361 caaaaagtcg cgtctcccga gagcctacag cggttggtgg cgaagacacc gccggaaaag
    15421 aaagtggaac tgaaagtttg gcgggaaggg aaagagtcca cgatctcctt ggtgaccggc
    15481 aaaatgcccg aatcgacggc cgaaggggag gcggggccct cgaactcctc cgacggcgcc
    15541 gggaaggaaa agcaatcgga gtccttcgat tgggagggcg ggcatttcgt cccgaattcg
    15601 gccgcttggg ccaagcgtta cgacctgccg gaggatgccc ccggggtggt gctggcgcgc
    15661 ctggagccgg gaagtttggt ggaggccgtc ggcctgacgc cgggggactt gatcgcttcc
    15721 gtcaaccggg aagccacacc ggacctcgat cggttcaaga aggcgctcaa aaaagtcgac
    15781 tttaaatccg gcgccatgtt cgacatctat cgccaggggc ggtggttcta tctgagttat
    15841 cggaaagagg aatgaggcgg aaggaaaaaa cgcgggagaa cgttttaagg cgtccggttc
    15901 cccattttct tcagggagtt gaggaaggcg gaaacgaccg tcagcaccag gctgtaggcg
    15961 agggcccacc agaaactgtc cacggaaaaa ccgggcaccc accacgccac cagcatgatc
    16021 agcgccgcgt tgatgacgaa aaggaacagg ccgagggaca ggatgctgat cggcagggtg
    16081 atcagcacca ggaggggttt gatgaccacg ttcaccagac ccaggacgat ggccaccacc
    16141 acggccgtga ggagtttgtc cacatgcacg ccgcgcagca ggtaggcgcc cgtgaggacg
    16201 gccagcgtgt tgacgaagag atgggcgatc aggctcatca tgggtctgga aaatctacaa
    16261 tttttccgtg acataaaaaa gagccgcgtc cgcccggagg cgggtttcat gtgaaaatgg
    16321 taaaatcacg gtcttctcat gaaaacgtct ccttttaggc tcaccggccg cggcgcggac
    16381 accaacccgc ggaaccgttt ttcttcgacg cattccgtcc ccgagccgga ggacgccgcc
    16441 ccgccggacc ttcctccgaa gacccgtttc ctccgggaca cttcccgccg cgtcatttcc
    16501 tgccaccaca gtcccgatct cggttacggc gccacgctga acccctatcg cggctgtgag
    16561 cacggctgcg tgtattgttt cgcccgcccc acgcacgaat atctcgaccg gtcggcgggg
    16621 ttggattttg aaagcgtcgt tttcatcaag cacgacgcgg cgcggctcct gcggcgggag
    16681 ctgggggctc cccgatggga accccggacg gtggcgatgt gcggggtgac cgatccctac
    16741 cagcccatcg aacgccgccg ggaactgacg agggagtgcc tgaaggtgtt cctggaattc
    16801 cggaaccccg tcaccgtcac cacgaagaac cagctcgtca cccgggacag ggacattttg
    16861 tcggctttgg ccgggtggaa cgccgcggcc gtttttattt cggtgaccac gctggacgag
    16921 gaactctgcc ggcggatgga accgcggacg tcgcgtccgg cgcaccgcct ggaggccgtg
    16981 aaggccttgg cccaggcagg catcccggtg ggcgtcaatg tggcgccggt catcccgggg
    17041 ttgacggacc acgagctgcc cgccatccta aaagaagccg cgctggccgg cgcgaaatac
    17101 gcctggtacg cgcccgtgcg cctgccctac ggcgtggggg agttgtttga acggtggatc
    17161 gagaaatatt ttccggaccg gaaaaacaaa atcctcggcc ggcttcggtc cctccatggc
    17221 gggaaattga acgattcctc tttcggcgcg cgcctgcgcg gccggggggt ctacgccgac
    17281 gaattggagg ggctgttccg tctttcctgc cggcgtctcg ggctgagaga cgatgggccg
    17341 gctctctccg cggcgtcttt ccgccgtccc gtcggtcggc aacttgccct tttttccgga
    17401 tccgcctcgg attcgggatg tcccggggat gtcgaaatga taaaatgaac cggccccggc
    17461 gttcgctgtt ttcgccagga aacacagagt ttcggtttct tttccgcccg ggaaaatgag
    17521 gggagttttt aaacgtcaca tgaaaaataa atggtgcgtc atcggggagg ccgcccatta
    17581 aaccgcacgt ggtggtggtc ggaggcggtt ttgccggcgc ggcggccgcg tccgccctga
    17641 cggagagcgg cgtcacggtc acccttctgg agcgtcgtcc cacgctggga ggccgcacct
    17701 cctcgttccg cgacggcgtg acccgggagg aggcggacaa cggacagcat ctcttcatgg
    17761 gttgttataa ggaggtgcgc cggtttttgc ggcgtttgaa ggtgggggat cgcgttcaat
    17821 tttttccctc gttggacgtg aagttcctgt cgcccgccgg cgccgcggaa ctggcttgcc
    17881 cggttttggc gggccacctg ggcgttcttt gggggatcgg ccgctacggg gccatgtcgg
    17941 tgggggaccg gttttcggtg ttgagggccc tgtccggtct gcggttgaga aagcggtggg
    18001 gcgacttgaa gaacgtcacg gtgtcccagt ggctcgccgc gctgcgccag ccggcggggg
    18061 cccgccgggc tttttggacg ccgctgtgtt tggcgaccct caacgaacgt ccggaaaaag
    18121 cctgcgccga ggcgctggcc gtcgttttga gggaaggttt tttttcggac agttcctcct
    18181 cggccgtggg gtattgcacc gtggctctgg gcaaactgtg gtcggtggag ctgttcgcgt
    18241 atctgaaacg gcagggaggg gtggtggcgt cgcgtcaggc cgcccggggg ttcgaggcgc
    18301 aggacgacct ggtgagaagc gt
//
LOCUS       DAOTFD010000028        18103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2079_length_18103_cov_5.104000, whole genome shotgun sequence.
ACCESSION   DAOTFD010000028 DAOTFD010000000
VERSION     DAOTFD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 18103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18103
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_18103_cov_5.104000"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5079_03220"
     CDS             <1..482
                     /locus_tag="P5079_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29028.1"
                     /translation="HYRWIVEKAPRDNWQRFRAEMSLVQLKRQGGGKREEVLKEYERL
                     LGAYGVEANTAAEIHDSMAQVLMEMGKPEEAGKHYRWIVEKAPRDNWQRFRAEMILIE
                     IELHAKGRTREAIQAYLELLSQHKKDPEIVRNVRQTVGQLYLEVGEEKEAKKYLSDQ"
     gene            complement(511..2850)
                     /locus_tag="P5079_03225"
     CDS             complement(511..2850)
                     /locus_tag="P5079_03225"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29029.1"
                     /translation="MIPFLSFFTVFALYLHTLYPCIAPEDSGEFVTAAWTLGIPHASG
                     YSLYVLLGKIATVLFPFGNPAYRVNILSALLGAGTLFFMVNAFGRLLPPQNSAATLPA
                     QVEESRWRKTSLWLTALLWAMVPGVWYLSLLAEVYILELFLGMFALGCLIRCIVDDPL
                     PRRVYLRRWGLFSFLVGIGLSVHHTLVLWLPGLLGFVLGHKRAPPAKPASVVKTMTAP
                     TPTPFYRSDRAVLVCVSALLLAAGFSLQLYLLLRAHSSPLLNTGMPDNFVRWVRVLLR
                     MDYGTFSLSHADAATSWVWGMKNYLLGCLHAFTFLGAPLLFWGMLQMARQSKSVFWGT
                     VLLWFVPGPFFILIGRSPLQEHFQGVVERFYPLSFVGLALFLAWGIYDSGLRFAWFRR
                     ASFLLLPLSLLSHLWVNGRGNLHARDFGANLLRSLPPNSVLWDPGDTAAYAVLYQQTA
                     LGQRNDVASVCYHTTRWGQESLFQRYPDLLSPGTAVFQKNFLAGILNGPRREWVFVEV
                     PKSLPGMTGELPQTTRYPEDGLPLGIAYRYFPAASHRLIEPNSQLSLAALNFWWYRRS
                     APWRLSPSSPLWRGEDWFTIEIVRRYAEAHTNLGQKFAQYGIHDAAEKEYILALAADP
                     RQMEAYNNLGVLAYEGQNPAAAAVYFERALRQAPDNPSLYVNLGLSRKLAGENDKALE
                     AFRAALRLDPATTEARYQLADLLEARRDYTGALDEWKELQRRGQDDKTVYWKMAQLHA
                     ALNTKNKALENLSSYFLFDLSAEEKTAAEEFRRSLLEQN"
     gene            complement(2890..4995)
                     /gene="priA"
                     /locus_tag="P5079_03230"
     CDS             complement(2890..4995)
                     /gene="priA"
                     /locus_tag="P5079_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRY29030.1"
                     /translation="MSDGDTRPPALPSPPAAVPDSLVDVAEVAFPLPVPQTFDYLIPP
                     TLQGALRPGQRITAPFGHRGAQVGVVLKICRKDTSQRPSAALKSLLTAVDPIPALSEK
                     DILLARRLSTRYFCSIGQAIFCVLPIGKHLPPKRPKVSASAATAATAQKFTLTPDQQN
                     ACEKIIPRVEQGGFHPMLLWGVAASGKTEVYLRAIEAALKKGNSALYLVPEIGLTPQV
                     EALLRGRFGDLVEVWHSEIARGDRWRTWENALAGKCRVLLGPRSAVFVPLQNLGLVVM
                     DEEHDPSYKEDSAPRYHAREVALEKGRLHGVPVILGSATPSMESYRRALEGEIALVEM
                     SHRVFDRPAPDVRAADMRRRGGKLLSPELAESIAERLARKEQCLIFLNRRGFSTYVTC
                     AACGWEARCPRCAVSLVFHKAQAPMKPGGELLPAATKNPNDLHCHYCGYGMPLPERCP
                     RCRSAEIQLKGRGTQKIVSELALWFPQARFLRWDRDTTRKKGAHTEAFDAVRLETVDI
                     IVGTQMIAQGLDFPAVTLVGVLDADRTLRFPDFRAGERTFQLLTQVAGRAGRAEKPGI
                     VFLQTRSPDHYAVRAALRLDYKTFAEEELKFRREMEYPPYKRLVQILLRGKTAERVEH
                     AAEELMRWIEERSDWPKEVEPLGPAPSFYVLRAGLTQWQVLLKGPENVMELALSALRD
                     YKPPKLVSLLVDADPEELQ"
     gene            complement(4988..5776)
                     /locus_tag="P5079_03235"
     CDS             complement(4988..5776)
                     /locus_tag="P5079_03235"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY29031.1"
                     /translation="MAPSNARQDLLAMVQSLQDLLPPSDHSVLGHWAPHAGPAAKAVS
                     PPPSARPEDKLRQLLEKQIGDCHRCPLGDTRQKIAFGVGNPRARVMLVGEGPGYMEDR
                     QGEPFVGPAGQLLDKILAAIELSRQPVEPSWRWVYIANIVKCHPMKEPEQPDKRGNDR
                     PPTPEEMDACFPFLAEQIRIVRPWIILSLGATAAKSLLKTSRGITALRGQWFDFHLDG
                     CDPMRLMPTFHPAALLRDPALKRDVWEDVKKLSGDLKRRLSSDV"
     gene            complement(5782..6456)
                     /locus_tag="P5079_03240"
     CDS             complement(5782..6456)
                     /locus_tag="P5079_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRY29032.1"
                     /translation="MASQNFWRGKKIVITAGPTREALDPVRYLSNASSGRMGYALADA
                     ARKNGAAVVLISGPTALPPPRAVRLVPAVSARDMLRVALRHARNAHAVIGTAAVADWR
                     PARPRRHKMKKTGSADELLLRLKKNPDILKTLSHRRRDRRRPLLIGFALETDHLLQNA
                     RKKLSEKNLDLIVANAGSALDSPRTSVVLLVPSGILARFRNLSKKTAARRLLTAAARY
                     FERRRP"
     gene            complement(6467..7045)
                     /locus_tag="P5079_03245"
     CDS             complement(6467..7045)
                     /locus_tag="P5079_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRY29033.1"
                     /translation="MKKPSLRKREIVLGVTGSIAAYKAAELARRFQDGGARVTCVMTA
                     TATKFIAPLTLSALTGRPAAQDMFDQKLWNMSHLSLAQTADAVVVAPCSANVIGKLAA
                     GLADDLLTTLVLAAQVPVFIAPAMHKAMWDHPATRKNVETCRSYGYRFVGPVEGPLAS
                     GASGVGRMEEPSRIVETVAAALSTPAKRVRTS"
     gene            complement(7091..7426)
                     /locus_tag="P5079_03250"
     CDS             complement(7091..7426)
                     /locus_tag="P5079_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29034.1"
                     /translation="MTEKKKKSEAAVAEKKENLPLTQLLLDCPKDKYRLVSLAIRWAQ
                     EVKKRDQSTEAPVEVVNKSLKEILSDQVKLDDIEKLPPVPKTEKKPDLIPLTPKPEDK
                     PEEAPKKGE"
     gene            complement(7423..8043)
                     /gene="gmk"
                     /locus_tag="P5079_03255"
     CDS             complement(7423..8043)
                     /gene="gmk"
                     /locus_tag="P5079_03255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRY29035.1"
                     /translation="MSTGLLLVVSGPSGAGKSTLCQALIAKWPNLRYSVSCTTRPRRE
                     KEVDGRDYHFVSEDAFQKNIRDGKFLEWARVHDSFYGTLQEPLMEHLRQGLDVVLDVD
                     PHGALSIKEKFADCVGVYICPPTWESLEDRLRSRAQDDELSISKRLANARKEMGYLSH
                     YDYLVVNKVFRESLENLSAILRAEHSRVKRLSHELTYLEIMEEEKA"
     gene            complement(8107..9972)
                     /locus_tag="P5079_03260"
     CDS             complement(8107..9972)
                     /locus_tag="P5079_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29036.1"
                     /translation="MKNLLRKFFRRRIPLPENIPPAAEFSAFVLLLGLFLLFTLRCFH
                     SPGFIIDNLANEYKSAHFAEALRSGTFGLRDVLRQFHPPLYHGEMTTLLMLPFFYVWG
                     RGFLLVKLWPIVAGGLTVLLAYFLARKMFSGRVALLSTFLLVLHPSFILGVKSGNFQV
                     SYMILFSTGSVFLFFLWWTSHRTMYIFLGMVLVGLGIGTRMWFVWYVLALILCALLFF
                     KELWSRGFFRQKKRLLLGSALGIAGFVLGGFFTLRTEIAEGRIWDAVHSRINKEAFAP
                     AETVRSPRLALITARWEDLAGLLKGQTFFHLLFDRNIPRSMGNFLYPWLFWASFFFLL
                     YSVVLRNNSRKKRTLFLLTLFLSMFVVTYLTPRSRFNYHMFLYYPFPQLLIALAVGEA
                     AARLRPKSLLPRLFLLLFIAATFQETVALAYSLKHIKIFGGMRGFSDSLGDLHDWLMK
                     NRGDKRFFIMPTAIHETLYLYYPDFSLEDSHHTDILRPAFSQQLIEISPWKRYFSDNE
                     SNAAIRHGSPEERVPCRKKLNEYISTANLWPSFVTTWSTVVSSSSFDKIVLVNSNSWS
                     FTDQYFYYLLSWWGRCNGAPLRLARRFDDKDGTPSFLVYELPLKDRAIREKLPFD"
     gene            complement(9982..11625)
                     /locus_tag="P5079_03265"
     CDS             complement(9982..11625)
                     /locus_tag="P5079_03265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29037.1"
                     /translation="MGMASWVTRCRRLLNQDLGSRRTETLLVLLLLVVFTAMEFVSLE
                     IPGMLFDVLHMELLTLDIMKLLDKASLWELVRTGLLPAGQNVYHGALISYLLLPFFSL
                     FGPHWLLVRAWPVAWAVFSLLLSYLFVRDFFDKKTAFLAILFLILSAPYVMGIKTISD
                     VSIMHCFAMGALLCMSRYINTRRPSFLAAGMFLLGLGLCTRLWFAWLVGALAATAVFF
                     SLEIVPWLRRLPQRLSVLAVLAVGSFLAGAALNIYRDVSSLFAAEHLIFAGLSPSNYF
                     GPARYFENIFLNIRELVSLLHGREFFAFQFGVESASFSNPLTSVLFGVSLAWGLLLPF
                     FREAPRRKQGFFVSLLFLFMFIQTPVSPSSVHAVHLLFLYPLVPVILASTFTGFFDIC
                     RRAKLLPLAVIGLLILDLSYDVRFLGRYFHYQSTVGGVKRHSDQIYHLADWFRAGRVS
                     ARNTFFLEHNTMSTLRFLLPDLPLNTPCVDLLSPTVRRRAARNTPPMTFIFNHHGLWS
                     DKLPDSWRNFLSAAGRTAVSEKQFCDADGTPVFDIYTLR"
     gene            complement(11627..12592)
                     /locus_tag="P5079_03270"
     CDS             complement(11627..12592)
                     /locus_tag="P5079_03270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY29038.1"
                     /translation="MPEEKRFVFSGPKARLDKFLADRLAPLSRTRVQKLIESDIVFVN
                     GRTEPGRRLLEDGDVVTVTLPEFAALPAAAEGRIPVLHEDPALIVVDKPADIVVHPAG
                     PHRQGTLVQRLWPKLAPAWDKTVRAQGGAFDRPGVVHRLDKGTSGVMVLAKTPHAAEN
                     LSRQFAERTVKKTYWALVRGIPRTDSGRIQSVVGRSRRHPGRMTVKEEGRWSETDFKV
                     LERYPSSQTALLELHPLTGRTHQIRVQLAALGHPVVGDALYGDTAASQEARPLLHARS
                     LEFTHPARKRRMTFQAEPPKDFLLFMRRQKKIGRPPHARRNRTDR"
     gene            complement(12585..13352)
                     /gene="lgt"
                     /locus_tag="P5079_03275"
     CDS             complement(12585..13352)
                     /gene="lgt"
                     /locus_tag="P5079_03275"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRY29039.1"
                     /translation="MWPTLLPLGPVTLHTYGALIAVGFLAGYFYTVRLARKHGLSADT
                     VTDLCWVALLAGIIGARLLYVAFDWAYYSRSPLSVLKLWEGGLVWYGGFLFAVLSGIF
                     WLRRRRISVLLAADLLAPGTALGHAIGRLGCFAAGCCYGRPTQLPWAVRFTHPDTLAV
                     PHTPLHPSQLYESFLTFGIFAVLSGMAWRRPDRVGRGEVALVYLGLYAAARFVVEFSR
                     GDDRGPVLALLTTTQWISLAFLLAAMAGGVLLRRKNA"
     gene            complement(13358..13927)
                     /gene="lspA"
                     /locus_tag="P5079_03280"
     CDS             complement(13358..13927)
                     /gene="lspA"
                     /locus_tag="P5079_03280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRY29040.1"
                     /translation="MAGRRERKRALRPLRRRGEVLRRKSRPGGLLNFRRFAVLIVVVL
                     AADQLSKWLVLRFIPLHFSKPLLPFFCLTHVRNTGAAFGVYPQGNIFFIVMTLLILVI
                     LMLYHRLLSGSFGSALGLALVWAGALGNLVDRLFRGSVVDFLDFFVGTWHWYTFNVAD
                     SAISIGIALLLLDSFRPKAPADEPKTIGN"
     gene            complement(13834..16632)
                     /gene="ileS"
                     /locus_tag="P5079_03285"
     CDS             complement(13834..16632)
                     /gene="ileS"
                     /locus_tag="P5079_03285"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRY29041.1"
                     /translation="MKKPKYTVNLPKTNFPMKADLPQREPGTLSLWERLRLYEKLLAQ
                     PAPKGDFILHDGPPYANGRIHMGHALNKILKDIIVKYKSMSGYHAPYVPGWDCHGLPI
                     EHQLMKEKGWDKRKVGRVPFRKEAEKFAEHFIDLQRREFKRLGVLGDWEHPYKTLSPD
                     YEASIVRTFYDLLEKGFIYRDKKPVYWCASCETALAEAEVEYEEKNSPSIYVKFPVLE
                     WPGNPEAQALAKQAGKRLSVLVWTTTPWTLPANVALAFHPEQTYKLWEAPDGEKILVG
                     DPGWAFLEQTLGKSPREPFAIDGLSLEGLLCKNPLNNNESQGVLAEFVSKEEGSGVVH
                     IAPGHGQEDYGVGREYGLPTLSPVADDGRFADEVLPNVLKGQKVWDANAVIIELLAKS
                     RLLVKEQKITHSYPHCWRCKNPIIFRATEQWFLRVDDAFRRKLIASTDDVAWTPDYGK
                     ERLLGMLKTRPDWCLARQRYWGTPIPMFYCESCKEPVKNKDIFNIVVELFRKNGSNAW
                     YEKEPKDILPPNTKCSKCGKTSFRKEDDILDVWFDSGVSWAAVLRDRMKIPDRENVMY
                     LEGSDQHRGWFQTSLLPAMALTEKPPYKHVLTHGFVLDGEGRAMSKSQGNVIAPQEIM
                     EKHGADIVRLWVAMTDYREDVRLSQPILDRVIDTYRKIRNTLRFLLGNLSGFGPAKVA
                     VPYEKMETLDRGVLASLHSLAADVRKNYDNSEFHLVSNKICNDFCINLLSAFYLDVRK
                     DILYCDRPDSPRRRSAQTAFLEISKTLAQLLAPLLSFTAEEVWRTLSEQKLLSTEDNA
                     DSILLNPFASAGAVKKEDLDLFNDLANLKTSINSDVEKERQSGNIKGANDTHVILAFD
                     KGSRLSRVPAAELASYMNVAKITIEESGPSPVLKAAPGTKCPRCWVWREDVNESGLCG
                     RCADAEKFFAEKAAQEAS"
     gene            complement(16681..17766)
                     /gene="mtnA"
                     /locus_tag="P5079_03290"
     CDS             complement(16681..17766)
                     /gene="mtnA"
                     /locus_tag="P5079_03290"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRY29042.1"
                     /translation="MPRRRTAARRDHSKIKTFRWTGTSLFILEQRALPHKTRYIPCRT
                     HRDVARCIKDMAVRGAPAIGCVAAYGLALAARERNFRSVERLVAHLEKARVVLGATRP
                     TAVNLFWALNRMQRLWGTSPLSPKGPKGLEAALAKEAVAIHREDLAACRRMGELGAKL
                     LPKNAVVLTHCNAGALATAGYGTALGMVRAAFAQGKIKKVFVDETRPYLQGARLTAWE
                     MHQEGIPYEVITDNMAGHLMKTEKISAVIVGADRIAANGDAANKIGTYSLSILAKHHK
                     IPFYVVAPFSTVDMSMPTGQGIPIEERSTREVVFVGPTQIAPKGAKARHPGFDVTPAA
                     HIAAIVTDRGVVRPVSAKNLARVAKSK"
     gene            complement(17787..18050)
                     /locus_tag="P5079_03295"
     CDS             complement(17787..18050)
                     /locus_tag="P5079_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRY29043.1"
                     /translation="MMIRVRVIPNASRNEVVGRIGSTVRVKVAAPAIDGKANAELISF
                     LAEFFDVRNRGVKIVRGQKGKEKTIEIGGRAEEELEDVMETIP"
BASE COUNT         3661 a         4986 c         6077 g         3379 t
ORIGIN      
        1 aacattaccg gtggatcgtg gagaaggcgc cgagagataa ttggcagcgg ttccgggcgg
       61 agatgagcct ggtgcagctg aagaggcagg gcggagggaa gcgggaagag gtcctcaagg
      121 aatacgagcg gctgctgggg gcgtatgggg tggaggccaa cacggcggcg gagatccacg
      181 acagcatggc gcaggtgctg atggagatgg ggaagccgga ggaggccggg aaacattacc
      241 ggtggatcgt ggagaaggcg ccgagagata attggcagcg gttccgagcg gagatgattc
      301 ttattgaaat cgaacttcat gccaagggac gaacgcggga agcgattcag gcctatctgg
      361 aacttttatc gcaacacaag aaagacccgg agattgttag aaacgttcgg caaacagtcg
      421 ggcagcttta cctggaagtc ggagaggaaa aagaggctaa aaagtattta tctgatcaat
      481 agcgccgccg gcggatgctg cgtgccggtt tcagttttgt tctaaaagag atcgtctgaa
      541 ttcctccgcc gctgtttttt cttccgcgct cagatcaaaa agaaaataac tcgaaagatt
      601 ttccagcgct ttgttttttg tattgagcgc cgcatgaagc tgagccattt tccagtagac
      661 tgttttgtcg tcctgccccc gacgttgtaa ttccttccac tcatccagcg ctccggtgta
      721 atccctgcgg gcttccaata aatcagccag ctgataacgg gcttcggtag tggccggatc
      781 taatcggagg gccgcgcgga aggcttccag cgccttgtcg ttctctccgg ccagttttcg
      841 ggataagccg aggttgacgt acagagaggg gttgtccggg gcttggcgaa gggcccgttc
      901 gaaatagacc gcggcggccg ccgggttctg cccctcgtag gccagaaccc ccagattgtt
      961 gtaggcctcc atttggcgag gatccgcagc caacgccagg atatattctt tttcggcggc
     1021 gtcatgaatg ccgtattgcg caaatttctg ccccaaattc gtgtgcgcct ccgcataacg
     1081 ccggacgatt tcgatggtga accaatcttc tccgcgccac agcggggagg agggggagag
     1141 ccgccagggc gcggaacggc ggtaccacca gaaattcaac gcggcaaggg acaattggga
     1201 attcggttcg atcaggcggt gggaagccgc gggaaaatat cgataggcga tccccagggg
     1261 caggccgtcc tctggatatc gggtcgtctg cggaagttct ccggtcatgc cggggaggct
     1321 tttggggact tcaacgaaga cccattcccg ccgaggaccg ttgaggattc cggcgaggaa
     1381 atttttctga aacaccgcgg ttcccggcga cagaaggtcc ggatatcgtt ggaacaggga
     1441 ttcttgcccc cagcgcgtgg tgtgatagca gacggaggcg acatcgtttc tttggccgag
     1501 agccgtttgc tggtaaagaa cggcgtaggc cgcggtgtct cccgggtccc agaggacgga
     1561 gttcggcgga agcgaccgga ggaggttggc gccgaagtcc cgggcgtgga gatttcctct
     1621 cccgttgacc caaagatggg aaaggagact cagggggagg agaaggaacg aggctcgccg
     1681 gaaccaggcg aatctcaggc cgctgtcgta tatcccccac gccaaaaaaa gcgccaaccc
     1741 gacgaaggag agggggtaaa aacgttccac aaccccctga aaatgttcct gcaggggcga
     1801 ccggccgatg agaatgaaaa aagggccagg aacgaaccac aaaaggaccg ttccccaaaa
     1861 aacggatttt gattgacgcg ccatctggag cattccccag aaaagaagcg gcgctcctaa
     1921 aaaggtaaag gcgtgcagac agccgaggag gtagtttttc attccccaaa cccaagaggt
     1981 ggcggcgtcc gcatgggaca aagaaaacgt cccataatcc atacggagga ggacccggac
     2041 ccaacgaacg aaattgtcgg gcattcctgt gttgaggagg ggggacgagt gcgctcgaag
     2101 gaggagatag agttggagcg agaagccggc ggcgaggagc agggccgaca cgcaaaccag
     2161 gacggcgcgg tccgagcggt agaacggggt gggagtgggg gcggtcatcg ttttgacgac
     2221 cgaggccggt ttggcgggcg gggccctctt gtggccgagc acgaacccca aaagaccggg
     2281 cagccaaagc accagcgtgt ggtgaacgct caggccgatg ccgaccagga aagagaaaag
     2341 tccccagcgc cgcagataca cgcggcgagg gaggggatcg tcgacgatgc accggatgag
     2401 acacccgagg gcgaacatcc cgaggaataa ttccaggatg taaacctcgg ccaggaggga
     2461 gagataccag acgcccggga ccatcgccca cagcagagcg gtcagccaca agctggtctt
     2521 tcgccagcgg ctttcttcca cttgggccgg cagcgtggcg gcggaatttt gaggcggaag
     2581 gagccggccg aaggcgttga ccatgaaaaa cagggttccg gcccccagga gggcggagag
     2641 gatattgacg cggtaggcgg ggttgccgaa aggaaagagg accgtcgcga ttttcccaag
     2701 caagacatac agtgaataac cggaggcgtg ggggatgccc agggtccaag cggccgtcac
     2761 gaattcgccg gaatcttccg gagcgatgca gggatagagc gtgtgcaggt acagggcgaa
     2821 gacggtgaaa aaagacagga aggggatcat ggccgtgtca gccccggccc gttttgccag
     2881 acgacggggt cattggagtt cttccggatc ggcgtccacg aggagggaca ccaatttcgg
     2941 cggcttgtaa tctcggaggg ccgacaaagc aagttccatg acattttccg gacctttcag
     3001 caaaacctgc cattgcgtga gcccggctcg aaggacatag aagctggggg ccggtcccag
     3061 gggttccact tctttaggcc agtccgatct ttcctcgatc caacgcatca gttcttccgc
     3121 ggcatgttcc acgcgttccg ctgtttttcc gcgcaacagg atctgcacca ggcgcttgta
     3181 gggaggatac tccatttctc ggcggaattt caattcctct tcggcgaaag ttttgtagtc
     3241 gagtcggaga gcggcgcgga cggcgtaatg gtcgggactc ctcgtttgga ggaagacgat
     3301 tcccggtttc tccgcccgcc cggcgcggcc ggccacctgc gtcagaagct ggaaagtcct
     3361 ttccccggcg cggaaatccg gaaaacgaag ggtgcggtcc gcgtcgagga cgcccaccag
     3421 cgtcaccgcg gggaagtcca gcccttgggc gatcatctgc gttccgacga tgatgtccac
     3481 cgtttccagg cgcaccgcat cgaaagcctc cgtgtgcgcc ccttttttcc gggttgtgtc
     3541 cctgtcccag cggaggaatc gggcctgggg gaaccacagg gcgagttccg agacgatctt
     3601 ttgcgtcccc cggcccttga gttggatttc agcgctccga caccgcgggc atcgttccgg
     3661 caggggcatc ccgtatccgc aatagtggca atggaggtcg ttcggatttt ttgtcgccgc
     3721 gggaaggagc tctccgccgg gtttcatcgg cgcttgggcc ttatggaaga ccaggctgac
     3781 ggcgcagcgg ggacagcgcg cttcccagcc gcacgccgcg cacgttacgt aggtggagaa
     3841 accgcgacgg ttgaggaaaa tgagacactg ctctttgcgc gccaaccgct cggcgatgct
     3901 ctcggccagt tccggcgaca ggagcttccc cccgcggcga cgcatgtccg ccgcccggac
     3961 gtccggcgcg ggccggtcaa aaacgcgatg ggacatctcc accaacgcga tctcgccctc
     4021 cagggcccgc cgataactct ccatggacgg cgtcgcgctg ccgaggatca ccggcacgcc
     4081 gtgaagccgc cctttttcca gggccacctc tcgggcgtgg taacgcggcg ccgagtcttc
     4141 cttataggag gggtcgtgtt cctcgtccat caccacgagt cccaggtttt gaagaggcac
     4201 aaacaccgcc gagcgcgggc ccaggagaac gcggcatttc ccggccaggg cgttctccca
     4261 ggtccgccag cggtcccctc gcgcaatctc gctgtgccag acctcgacaa ggtccccgaa
     4321 ccggccgcgg agaagggctt ccacctgcgg cgtgagaccg atctccggca caaggtagag
     4381 ggcggaattc ccttttttaa gggccgcctc gatggcgcgg aggtagacct ccgttttacc
     4441 ggacgccgcg actccccaaa ggagcatcgg atggaacccg ccctgttcca cccgcggtat
     4501 tattttctcg caggcgtttt gctggtcggg cgtcagtgtg aatttttgcg ctgttgcggc
     4561 cgtggcggcg gaggccgaca ctttcggtct tttgggagga agatgtttcc cgatgggaag
     4621 gacgcaaaaa atcgcttggc cgatggagca aaaataacgg gtactcaacc ggcgggccag
     4681 aaggatgtcc ttttcggaaa gcgccgggat cgggtcgacc gcggtcaaca gcgatttcag
     4741 cgccgccgac ggccgttgtg acgtgtcctt tcggcagatt ttcaggacca cccccacctg
     4801 cgcgccccgg tgcccaaaag gggccgtgat ccgctggccc ggccgaaggg cgccttgcag
     4861 cgtcggtggg atcaggtaat cgaacgtttg gggaaccggg agggggaacg ccacctccgc
     4921 cacatccaca agggaatcag ggaccgccgc tgggggggaa ggaagggcgg gaggacgcgt
     4981 gtcgccgtca gacatcggac gagagacgtc tcttcagatc gccgcttaat tttttcacgt
     5041 cctcccaaac gtcccgtttc agggcggggt cgcgcaggag cgcggccgga tggaacgtgg
     5101 gcatgagtct catggggtcg catccgtcca gatggaaatc gaaccattgc ccgcgcaggg
     5161 cggtgatgcc gcgcgaggtc ttcaggagcg acttggccgc ggtggctccc agagagagga
     5221 tgatccaggg ccgcacgatc cgtatctgct cggcgaggaa ggggaaacag gcgtccatct
     5281 cttcgggcgt gggcggccgg tcgttgccgc gtttatcggg ctgctcgggt tccttcatgg
     5341 gatggcattt gacgatgttg gcgatataga cccatcgcca cgacggttca acgggttgcc
     5401 tggaaagctc gatggccgcc aatatcttgt ccaacagttg tcccgccggg cccacgaagg
     5461 gttctccctg gcggtcttcc atataaccgg gaccttctcc aacgagcatc acgcgcgccc
     5521 gcgggttccc gaccccgaag gcgatctttt ggcgcgtgtc tccgagaggg caacggtggc
     5581 aatccccgat ttgtttctcc agcaattgac ggagcttgtc ttccggccgg gccgagggcg
     5641 ggggcgaaac ggctttcgcg gccggccccg cgtgtggagc ccagtggccg agaacggagt
     5701 ggtcggaagg agggaggagg tcctggagag actggaccat ggccagaaga tcctgccgcg
     5761 cgttcgaggg ggccatcgac gtcagggccg gcggcgctcg aaatagcgcg cggcggcggt
     5821 taggaggcga cgtgccgccg tttttttgga gagattcctg aaacgcgcga gaatgccgga
     5881 ggggaccagc agaacgacgc tcgtccgcgg gctgtccagg gcggagccgg cattggccac
     5941 gatcaggtcc agattctttt cagacagttt tttccgggca ttttggagga ggtggtccgt
     6001 ttcaagggcg aatccgataa ggagaggccg cctccggtcc cgccgtcgat gggagagggt
     6061 cttcagaata tcgggattct ttttgagtcg aagcaagagt tcgtcggcag accccgtttt
     6121 tttcatttta tgacggcgcg gccgggccgg tcgccagtcc gccaccgccg ccgtgccgat
     6181 gacggcgtgg gcgttccggg cgtgacgcag cgccacgcgg agcatgtccc gcgcggagac
     6241 ggctggaacc agacgtacgg cgcgcggggg ggggagggcc gtggggccgg agatcaggac
     6301 gacggccgcc ccgttttttc gggcggcgtc ggcgagggcg tagcccatgc ggccggagga
     6361 ggcgttgctg agataccgga ccgggtccag cgcttcacgc gtggggcctg ccgtgatgac
     6421 gatttttttc ccccgccaaa agttttgcga ggccataagg ggggggttaa gacgtgcgga
     6481 cgcgtttggc gggagtggag agggcggccg cgacggtttc cacgatgcgg gaaggttcct
     6541 ccatccgtcc aacgccgctg gcgccggagg ccagagggcc ttccaccggc ccgacgaagc
     6601 gatatccgta agagcggcac gtctcgacgt ttttgcgggt ggccggatgg tcccacatcg
     6661 ccttatgcat ggccggggcg atgaagaccg ggacctgggc ggcgaggacg agggtggtca
     6721 ggaggtcgtc ggccaatccg gcggccagtt tgccgatgac attcgcggag cagggggcca
     6781 ccacgacggc gtcggcggtt tgtgcaaggg acagatggga catgttccag agtttctggt
     6841 cgaacatgtc ctgggcggcg ggccgtcccg tgagagcgga aagggtgagg ggcgcaatga
     6901 atttcgtcgc ggtcgctgtc atcacgcagg tgacgcgcgc tccgccgtcc tgaaaccgcc
     6961 gcgccagctc ggccgctttg taggcggcga tgctgccggt cacgccgagg acgatctcgc
     7021 gttttcggag agagggtttc ttcattcggt tgggtccggg cgaaatggcc gccgagaagg
     7081 cgaagagagc ttattcgcct tttttgggag cctcttccgg tttgtcctcc ggtttggggg
     7141 tcaacgggat gaggtccggc tttttttccg tcttcggcac cggcgggagt ttttcgatgt
     7201 cgtccagttt cacttgatcc gacaggatct ccttcagcga tttattcacc acttccacgg
     7261 gggcctcggt ggactggtcg cgttttttga cctcctgggc ccagcggatg gccagcgaaa
     7321 cgaggcggta tttgtctttc ggacaatcca gcaacagttg agtgagcgga agattttcct
     7381 ttttttctgc cacagccgct tccgatttct ttttcttttc ggtcacgctt tttcctcctc
     7441 cataatttcc aaataggtca gctcgtggga caagcgtttg acgcgcgaat gttccgcccg
     7501 cagaatggcc gacaggttct caagggattc acggaaaacc ttgttgacca ccagatagtc
     7561 gtaatgggac agatacccca tttctttccg ggcgttggcc agccgtttcg agatcgaaag
     7621 ttcgtcgtcc tgggcgcggc tccgaagtcg gtcttccagg gactcccagg tgggcggaca
     7681 gatgtagacg ccgacgcagt cggcaaattt ctccttgatg gacagggcgc cgtgcggatc
     7741 cacgtccaac accacgtcga gcccttggcg gagatgttcc atgaggggct cctgcagcgt
     7801 gccgtagaac gagtcgtgca cccgggccca ttcgaggaat tttccgtccc ggatgttttt
     7861 ctggaaggcg tcctcggaga caaagtggta atcccgcccg tccacctctt tttcccggcg
     7921 cggacgggtg gtgcaggaga ccgaatagcg aagattcggc cactttgcaa tcagtgcttg
     7981 gcaaagagtg gatttgccag ccccggaggg tcctgatacg actaagagaa gaccggttga
     8041 catggattta ggcgtgtgga ggattccagt atagcttttc gccgcgaggg acgtcaatcg
     8101 gcgggatcaa tcaaagggca gtttttcccg gatcgcccgg tccttcaagg gcagttcgta
     8161 gaccaggaaa gaaggcgtcc cgtctttgtc gtcaaaacgg cgcgccaggc gcaagggggc
     8221 cccgttgcag cgtccccacc aagacagcag gtaataaaaa tattggtcgg tgaacgacca
     8281 ggaatttgaa ttgacgagga cgattttgtc gaaagaggag gacgagacga ccgtcgacca
     8341 ggttgtgacg aaagacggcc agaggttcgc cgtggaaata tactcgttca gttttttccg
     8401 gcaggggacg cgctcctccg gggagccgtg ccggatggcg gcattggact cgttatccga
     8461 gaaatatctc ttccaaggag agatttcaat gagctgttgc gagaaggcgg gccggagaat
     8521 gtccgtgtgg tgggagtctt ccagggaaaa atccggataa taaaggtaaa gagtttcgtg
     8581 aatcgcagtc ggcatgatga aaaatctctt gtctccgcgg tttttcatca gccagtcatg
     8641 gagatctccg aggctgtcgg aaaagccgcg catcccgccg aagattttaa tgtgtttcag
     8701 cgagtaagcc agcgccacgg tttcctggaa ggtcgcggcg atgaaaagga gcaggaaaag
     8761 gcgcggaaga aggctcttgg ggcggagccg cgcggcggcc tcgccgacgg ccagggcgat
     8821 gaggagttgg ggaaaggggt aatacaggaa catgtgataa ttgaaacggg atcggggcgt
     8881 gaggtaggtc acgacgaaca tcgagaggaa caaagtcaga agaaaaagag tccgcttctt
     8941 ccgggagttg ttccgaagga cgacggaata aaggagaaag aaaaacgatg cccagaagag
     9001 ccacgggtat agaaaattcc ccatgcttcg cggaatgttc ctgtcgaaca ggagatgaaa
     9061 aaaggtttgt cccttcaaaa gccccgcgag gtcttcccag cgcgcggtga tcagggccag
     9121 gcgcggcgaa cggaccgttt cggccggcgc aaacgcttct ttgttgatgc gggaatggac
     9181 ggcgtcccat atccgcccct cggcgatttc cgtgcggagc gtgaaaaatc cgcccaacac
     9241 gaacccggcg atccccaggg ccgaacccaa cagaaggcgt tttttctgcc gaaagaagcc
     9301 gcgcgaccag agttccttga aaaagaggag cgcgcacaga atcagcgcca gcacatacca
     9361 gacgaaccac atccgggttc ctatccccag cccgaccagg accatgccca ggaagatgta
     9421 catcgtccga tgggaggtcc accacaggaa aaagaggaaa accgagccgg tggagaaaag
     9481 gatcatatag gagacctgaa aatttccgct tttaacgccc aggatgaacg agggatggag
     9541 gacgagcagg aacgtggaga gaagcgccac ccggcctgaa aacatttttc gcgccaggaa
     9601 gtaggcgagg agcaccgtga ggccgccggc gacgatgggc cagagcttaa caagcagaaa
     9661 accgcgcccc cacacgtaga agaacggcag catgaggagc gtggtcatct ccccgtggta
     9721 gagcggcgga tggaattgcc gcagaacgtc gcggagcccg aacgtcccgg atcggagggc
     9781 ctcggcgaaa tgggcggatt tgtattcgtt ggccaggttg tcgatgatga atccggggga
     9841 atggaaacaa cggagagtga acagaaggaa gaggccgagg agcaggacga aggcggagaa
     9901 ttcggcggcg ggggggatgt tttccgggag cgggatgcgg cgccggaaga acttgcggag
     9961 gaggtttttc atggggggaa cttaacgcaa ggtgtagatg tcgaaaacgg gcgtgccgtc
    10021 ggcatcgcaa aactgttttt cggagaccgc cgttcgtccg gccgcgctca gaaaattgcg
    10081 ccaggaatcc gggagcttgt cgctccacag gccgtggtgg ttgaagataa acgtcatggg
    10141 gggcgtgttg cgggcggcgc gccgccgaac ggtggggctc aaaaggtcca cgcagggggt
    10201 gttgagcgga aggtccggca ggaggaaccg gagggtggac atggtgttgt gttccaggaa
    10261 gaacgtgttc cttgcggaga cgcggcctgc tcggaaccag tcggccagat ggtaaatctg
    10321 gtcggaatgg cgtttcacgc cgccgacggt actttgataa tgaaagtagc ggccgagaaa
    10381 acggacgtcg taggagagat caaggatcag caggccgatg acggccaacg gaagcagttt
    10441 cgctcgtcga cagatgtcga aaaaacccgt gaaggtggag gccagaatga ccgggaccag
    10501 cgggtaaagg aacagaagat gcaccgcgtg gacgctgctg ggggagaccg gcgtttggat
    10561 gaacatgaac aggaagagaa gggagacgaa aaacccctgc ttccggcgag gcgcttctct
    10621 gaagaatggg agcaagagtc cccacgcgag gctcacccca aagaggacgg atgtcagcgg
    10681 gttggaaaag gaggcggatt cgaccccgaa ctggaaggca aagaattccc ggccatgaag
    10741 aagggagacc agctcccgga tgttcagaaa tatgttttcg aaataacgtg cggggccgaa
    10801 atagttcgat ggggagaggc ccgcgaagat caggtgttcc gcggcgaaca ggctgctcac
    10861 gtcccggtag atattcaggg ccgcccccgc gaggaaagag ccgacggcca aaaccgccag
    10921 cacggacagt cgttggggga ggcggcgtag ccaggggacg atctcaaggg agaaaaagac
    10981 cgctgtggcg gcgagggcgc cgaccaacca cgcaaaccac aggcgcgtgc agagtcccag
    11041 cccgaggagg aacatccccg ccgccagaaa ggatggccgc cgggtgttta tgtagcggga
    11101 catgcagaga agcgcgccca tggcaaaaca gtgcatgatc gacacgtcgc tgatcgtctt
    11161 aatccccatg acgtaggggg cgctcagtat caggaaaagg atcgccagga aggcggtttt
    11221 tttgtcaaaa aaatcccgga caaaaagata ggagagcagg agggagaaga ccgcccaggc
    11281 gaccggccag gctcgaacga ggagccaatg ggggccgaaa agggaaaaaa acgggaggag
    11341 gagataggaa atgagggcgc cgtggtacac attctggccc gcgggcagca ggcccgtccg
    11401 gaccagttcc cagagcgagg ccttgtccag cagtttcatg atgtccaggg tcagaagttc
    11461 catgtgaagg acgtcgaaga gcatgccggg gatttccagg gagacgaact ccatggcggt
    11521 aaagacaacg aggaggagaa gaaccagaag ggtttccgtc cgacgggagc cgaggtcctg
    11581 gttcaaaagt cgccggcacc tcgtcaccca agaggccatt cccatcttat cggtccgtcc
    11641 tgttccggcg ggcgtgggga gggcgaccga tctttttctg ccgtctcatg aagaggagga
    11701 agtccttcgg cggttcggcc tgaaacgtca tgcggcgttt gcgggcgggg tgggtgaatt
    11761 ccagcgagcg ggcgtggagg agcggacgcg cctcttggga cgccgcggtg tcgccgtaaa
    11821 gggcgtcgcc gacgaccggg tggccgagcg ccgccagttg gacccggatc tggtgcgtgc
    11881 ggccggtgag cggatgaagc tcgagcaggg ccgtttgaga agacgggtac cgttccaaaa
    11941 ctttaaaatc cgtttccgac cagcgcccct cctctttcac cgtcatgcgt cccgggtggc
    12001 ggcgggagcg tccgaccacg gattggatgc ggccgctgtc ggtccggggg attccccgca
    12061 caagggccca ataggttttt ttcacggtgc gttcggcgaa ctggcggctc aggttttccg
    12121 cggcgtgcgg cgttttcgcc aggaccatca cgcccgaggt gcctttgtcc aggcggtgga
    12181 cgacgcccgg ccggtcgaag gccccgccct gggcgcgcac ggttttgtcc cacgcggggg
    12241 ccagcttcgg ccagagacgt tggacgaggg tgccttgccg gtgcggcccg gccggatgaa
    12301 cgacgatgtc cgcgggtttg tcgaccacga tgagggcggg atcttcgtgg aggacgggga
    12361 tccgtccctc ggcggcggcc ggaagcgcgg cgaattccgg cagagtgacg gtcacgacat
    12421 cgccgtcttc caggagccgt cgtccgggtt cggtccggcc gttgacgaag acgatgtccg
    12481 attcgatcag tttttggacg cgggtgcgtg agaggggggc cagtcggtcg gcgaggaatt
    12541 tgtcgagccg cgccttcggt ccggaaaaaa cgaaacgttt ttcctcaggc atttttccgg
    12601 cgaaggagaa ccccgcccgc catcgcggcc agaaggaacg caagggagat ccattgagtg
    12661 gtcgtgagaa gggccaaaac gggaccgcga tcgtccccgc gggagaactc gacgacaaaa
    12721 cgggccgcgg catacagtcc caggtagacg agcgccacct ctccgcggcc cacccggtcg
    12781 ggtctccgcc aggccatccc ggacagcacg gcgaagatgc cgaaggtgag gaaagattcg
    12841 tagagttgag agggatgaag aggcgtgtgg ggcacggcca gcgtgtccgg atgggtgaat
    12901 cgcacggccc atgggagctg ggtggggcgg ccgtaacagc agccggcggc aaaacagccg
    12961 agccggccga tggcgtggcc gagcgctgtg cccggcgcga gcaggtccgc ggccagcagg
    13021 acggagatgc gccgccgccg gagccaaaag atccccgaaa ggacggcgaa gaggaacccg
    13081 ccgtaccaca caaggccgcc ttcccagagt ttgaggacag agagcgggga acggctgtaa
    13141 tacgcccaat cgaacgccac atagagcaat cgtgcgccga tgatgccggc gagcaacgcg
    13201 acccagcaga ggtccgtgac ggtgtccgcg gacaggccgt gcttccgcgc cagacggacg
    13261 gtgtagaaat aaccggccag aaaacccacg gcgatgagcg cgccgtaggt gtgcagcgtg
    13321 acggggccaa gcggcagcag cgtgggccac atggggatca gttccctatt gttttgggtt
    13381 cgtccgcggg ggctttcgga cggaaggaat ccagaagcag cagggcgatg ccgatcgaga
    13441 tggccgaatc cgccacgttg aaggtgtacc agtgccaagt gccgacgaaa aaatccagga
    13501 agtccaccac gctgccgcgg aacagccggt ccaccaggtt tcccagcgcg ccggcccata
    13561 ccagcgccag ccccagggcc gacccgaacg agccggagag gagccggtgg tagagcatca
    13621 ggatgacgag gatgagaagg gtcatcacga tgaagaagat gttcccctga gggtagacgc
    13681 cgaaggcggc accggtgttg cggacgtggg tgaggcagaa gaacggcagg aggggtttgg
    13741 aaaaatgcag cgggatgaac cgcaggacca gccatttgga gagctggtcc gccgccagaa
    13801 cgacgacgat caaaacggcg aatcggcgga agttcaggag gcctcctggg cggctttttc
    13861 ggcgaagaac ttctccgcgt cggcgcagcg gccgcagagc ccgctttcgt tcacgtcttc
    13921 ccgccacacc cagcaccggg ggcacttcgt gcccggcgcc gctttgagca cagggctcgg
    13981 accgctttct tcaatggtta tttttgccac gttcatatac gaagcgagct cggctgctgg
    14041 gactctcgaa agtcttgaac ctttgtcaaa tgccaaaatg acatgggtgt cgtttgcgcc
    14101 cttgatattg ccggactgcc tctctttttc gacgtccgaa ttgatactcg tttttaaatt
    14161 ggccagatcg ttgaacaggt ccaggtcctc ttttttcact gccccggccg aggcgaacgg
    14221 gttcagaagg atgctgtccg cgttgtcttc ggtcgacagg agtttttgtt cgcttagtgt
    14281 ccgccagacc tcttccgcag tgaaagacaa gagaggcgcc agtaattggg caagagtttt
    14341 tgaaatttcc aggaaggcgg tctgggcgct gcggcgacgc ggcgagtccg gcctgtcgca
    14401 gtagaggatg tccttccgga cgtcgaggta aaaagcggag agcaggttga tgcaaaagtc
    14461 gttgcagatt ttgttcgaga caaggtggaa ctcggagttg tcgtaatttt tgcgcacgtc
    14521 tgccgccagc gaatgcaaag acgcgaggac gccgcggtcg agtgtttcca tcttctcata
    14581 cgggacggcg accttcgcgg gaccgaaccc cgacaggttt cccagcaaaa accgcagggt
    14641 gttgcggatc ttgcggtagg tgtcgatcac gcggtcgagg atgggctggg agaggcgcac
    14701 gtcctcccgg tagtcggtca tggccaccca gagccgcacg atgtcggcgc cgtgtttttc
    14761 catgatctcc tgcggcgcga tgacgttgcc ctgcgacttg gacatggccc ggccttcgcc
    14821 gtcgagaacg aaaccgtgcg tcaatacgtg tttgtagggc ggcttctcgg tcaacgccat
    14881 ggccggcaac agcgacgttt ggaaccagcc gcggtgctgg tcggaccctt ccagatacat
    14941 cacgttctcc cggtctggaa ttttcatccg gtcgcgcagc accgccgccc acgacacccc
    15001 cgaatcgaac cacacgtcga ggatgtcgtc ctcttttcgg aaggatgttt tcccgcattt
    15061 ggaacacttc gtgttcggcg gcaggatgtc tttcggttcc ttttcatacc aggcgttgct
    15121 gccgttcttt cggaacagtt cgacgacgat gttgaaaata tccttgtttt tcacgggctc
    15181 tttgcagctc tcgcagtaga acatggggat gggcgtgccc caatagcgct ggcgggccag
    15241 gcaccagtcc ggccgggtct taagcatgcc gaggaggcgt tccttgccgt agtccggcgt
    15301 ccaggccacg tcgtcggtgc tggcgatgag tttccggcgg aacgcgtcgt ccacgcgcag
    15361 gaaccactgt tccgtggcgc ggaagatgat gggattcttg caccgccagc agtgcgggta
    15421 ggagtgggtg attttctgtt ccttcaccag cagacgggat ttggcgagga gctcgatgat
    15481 gacggcgttg gcgtcccaca ccttctggcc tttcaatacg ttgggcagga cctcgtcggc
    15541 gaatcggccg tcgtcggcca cgggggaaag cgtgggcaat ccgtattcgc ggcccacgcc
    15601 gtagtcctcc tggccgtgtc ccggcgcgat gtgcactacc ccgctgcctt cttctttgga
    15661 cacgaactcc gccagcacgc cctgggattc gttgttgttg agagggttct tgcagagcag
    15721 cccttccagc gacaggccgt cgatggcgaa cggttcccgc ggggatttcc ccagcgtttg
    15781 ttccaggaac gcccacccgg ggtcgcccac caggattttt tcgccgtcgg gggcctccca
    15841 cagtttatag gtctgttccg gatggaacgc cagcgccacg ttggcgggga gcgtccacgg
    15901 cgtggtcgtc cacaccagca ccgagaggcg tttgcccgcc tgtttggcca gcgcctgggc
    15961 ctccgggttt cccggccatt ccagcacggg gaacttcacg tagatggacg gcgaattctt
    16021 ttcctcgtat tccacctcgg cctcggccag ggcggtctcg caggaggcgc accaatacac
    16081 gggtttcttg tcgcggtaga tgaacccttt ttccaggagg tcgtaaaaag tccggacgat
    16141 gctcgcctcg tagtcggggg acaaggtttt gtagggatgt tcccagtcgc ccagcacgcc
    16201 caggcgtttg aactcgcggc gctgcaggtc gatgaaatgt tcggcgaact tctccgcctc
    16261 ttttcggaac ggcacgcggc ccaccttgcg tttgtcccag cccttttcct tcatgagctg
    16321 gtgctcgatg ggcaggccgt ggcagtccca gccgggcacg tagggcgcgt ggtagccgct
    16381 catggatttg tatttgacga tgatgtcctt gaggatcttg ttgagcgcgt ggcccatgtg
    16441 gatgcggccg ttggcgtagg gtggaccgtc gtgcagtatg aaatcccctt tgggggcggg
    16501 ttgcgccagc aacttctcat agaggcgaag cctctcccaa agggacagcg tccccggttc
    16561 ccgctgaggc aggtcggcct tcatggggaa attcgtcttc ggcaggttga cggtgtattt
    16621 gggttttttc atgagtgaat cattgtagcg aatccgctgt tgctggaaaa gggcgacgga
    16681 ttacttcgat ttggcgacac gagccagatt ttttgcggag acgggtcgga cgacgcctcg
    16741 gtcggtgacg atggcggcga tgtgggccgc gggggtgacg tcgaacccgg gatggcgggc
    16801 cttggcgccc ttgggagcga tctgcgtggg cccgacgaag accacctcgc gcgtggagcg
    16861 ctcctcgatg gggatcccct ggcccgtggg catgctcatg tccaccgtgg aaaacggggc
    16921 caccacgtaa aaagggattt tgtggtgttt ggccaggatg gacagcgaat aggtgccgat
    16981 cttgttggcg gcgtcgccgt tggcggcgat gcggtccgcg ccgacgatga ccgccgagat
    17041 tttttccgtc ttcatgaggt ggccggccat gttgtcggtg atgacttcgt aggggatgcc
    17101 ttcctgatgc atttcccagg cggtgagacg ggccccctga aggtagggac gggtctcgtc
    17161 cacaaaaact tttttgatct tcccctgcgc gaaggccgcg cggaccatgc ccagcgcggt
    17221 gccgtatccg gcggtggcca gcgccccggc gttgcagtgg gtgaggacca cggcgttttt
    17281 cggcaggagt ttggcgccca gttcgcccat ccgtcggcaa gcggccaggt cttcccgatg
    17341 gatggccacg gcttctttcg ccagcgccgc ctccagccct ttcgggcctt taggcgagag
    17401 gggcgaagtc ccccagagac gttgcatgcg gttcagcgcc cagaaaaggt tcacggccgt
    17461 cggccgggtg gcgcccaaga cgacacgcgc cttttccaaa tgggccacga gccgctcgac
    17521 ggaacggaag ttcctttccc gggcggccag ggccagcccg taggcggcca cgcagccgat
    17581 ggcgggcgcg ccgcgcacgg ccatgtcctt gatgcagcgg gccacgtcgc ggtgggtgcg
    17641 gcacgggatg tagcgggttt tgtggggaag ggcgcgttgt tccagaatga ataaagacgt
    17701 tccggtccag cggaacgtct ttatcttgga gtggtcgcgc cgcgcggccg tccgccggcg
    17761 cggcacgcgc gccggggaag gtttcgtcac gggatggtct ccatcacgtc ctcgagttct
    17821 tcctcggcgc ggccaccgat ctcgatggtt ttttccttcc ctttttgccc acggacgatc
    17881 ttgacgccgc ggttgcggac gtcgaaaaac tccgccagga aactgatcag ttccgcgttg
    17941 gcttttccgt cgatggcggg ggcggccacc ttcacgcgca ccgtggaacc gatgcggccg
    18001 accacctcgt tgcgggaagc attgggaatg actctgactc gtatcatcat cgctctgtca
    18061 cctcaactgt tcgggattaa atcgttttaa cgccgggaaa gtt
//
LOCUS       DAOTFD010000029        17937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2106_length_17937_cov_4.744548, whole genome shotgun sequence.
ACCESSION   DAOTFD010000029 DAOTFD010000000
VERSION     DAOTFD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_17937_cov_4.744548"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="P5079_03300"
     CDS             <1..399
                     /locus_tag="P5079_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29044.1"
                     /translation="LAVMGSWAVRNPLMSLSTPLLILAIPIFDMIYTTVSRIRNGRVR
                     NVREWLEFVGRDHFHHRLMHLGMEERQTVGFILLVNLCLGLSSITVRYTATRLSALLM
                     LFQAFLIFMIITALMLLGREITPHPLKGKT"
     gene            404..3412
                     /locus_tag="P5079_03305"
     CDS             404..3412
                     /locus_tag="P5079_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29045.1"
                     /translation="MNGLFFRSCSKKARTVAGIVALALSLPAPAVQGAKPPAADAPTA
                     YTLEFTPRNVQRTLDDFLKFIQATAPATDGIRAQYLAAELYLQRGEFRRAAEIFQKLA
                     AVTFDDKYFNVSVLEKLADCYFRQGLFQQAADSYMTVRNSNVKAILPEALLGLSVSAL
                     ALGNREEAYLRIQELLAFHPAYANHVPLILPIGLVHWENGKFQEALDLFQRDPDNPAS
                     LYFAGLCQRSLDKPVDAMGTFKKVVQNHRKTVWGTRAKFELAETFYQKKDYPLASRTF
                     DQIYRGERDDAWETLALYRLACSDMHMRRFKDAETKLWPLQKKKKDHRLHPNVNYLLT
                     ESLAEQNKIEKMVPLLKAEIKGKGRTPDNIYRLIWSLAAIGDYAEVIRQSNEFLSTSW
                     DMELTPKTLVAQGYAYDKLGRTPESAASYQLVAENFSNTPYAARALQLTAMTYFRSKQ
                     HGLIVTQVNNQWNAVPLEIRQKNPETLFWIAEAHLQLENGAEAREHYKKFLDFAPPDH
                     PLGTQALMGEAVSYAVDKDFPTAILTLQKTLQSAQEKGNKPLMAASMLEMGNVFFNAK
                     DYENAASSYRSFREVDPKHAQAAFSLYQEGLALHRAEYYSDAIAAWDKLVKTHPKAPQ
                     ASEALFRKAKTLFDTGNYPGAVKDYEKLIGNYPKSEFAKDARLQIGQCFYNAGDFAKA
                     IVHYTDFLNRFPDDPLAPSVLQLLQTSYYQAKKTPDEIEKLTRNQPKSAILADIYWEE
                     GAKLYNEKSYDKAREYFQKILSEFPSSSLAPQAAFYRAESLYLQEKYFESIPAYENFI
                     RYYPEDAQRSLAMFHLSVGLFNQKEYAESAKAFGDFAAQFPDDPMAKNANLNVAVCYA
                     KAQDVNNAVAAYERYIALYPDGEDVGATYLQMGQLLEKFGQEARAADVYKRVPKNLPE
                     YAEALFNAGRCYRALNQLAGEQQAYEDLRLFAPKNDAYRTAGLLQLAEIHLTKNETAK
                     AAEIYRDVVANAADDQSRAMAEERLKTLGP"
     gene            3455..4081
                     /locus_tag="P5079_03310"
     CDS             3455..4081
                     /locus_tag="P5079_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY29046.1"
                     /translation="MHELTFIELMKISLVMPVLLVLSIVLVMVFLERWIFFTRMARVD
                     ASLFKRVKQAVREKKLKEAQQIASGGRGLLAQALEAELDAAQTGSREEIDTVLTLYYQ
                     RVQALLSRRLGIFGTLSFISPLLGLLGTVLGVMRAFRDLALSGSGGPTIVAAGISEAL
                     IATAAGIAVAVSAALIYNYFNFRLKYVLNSLNLFGQEILLLVVSGKEI"
     gene            4119..4583
                     /locus_tag="P5079_03315"
     CDS             4119..4583
                     /locus_tag="P5079_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRY29047.1"
                     /translation="MDDPSQSGEVEETEKINVVPLADLTLVLLIILMVLSPMITQSMI
                     NVATPAVKAEKDIEQKPQEEKKPVEPLLIALAESGYTLNNVPADSLDQLMAALRARLE
                     ETPDRPVLVTADQQITVGAVVEVLDLAKQNGAQKVSLLKRAGVEESAEKTAP"
     gene            4580..5002
                     /locus_tag="P5079_03320"
     CDS             4580..5002
                     /locus_tag="P5079_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRY29048.1"
                     /translation="MKKLARSFEDAPIAGINIVPVIDLCLVLLVILLILSPLMDAPNL
                     TIKLPEAKTKEEKENIISLSVTPDGKMALNTDLIEAGDIPKLLPVLLAEQGENTPVVI
                     RADKNVTYGRLTDLLKTVKTAGAKRISLGTEQPKGAAQ"
     gene            4999..6303
                     /locus_tag="P5079_03325"
     CDS             4999..6303
                     /locus_tag="P5079_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY29049.1"
                     /translation="MMRSWNLLYERQPLQLDVLLLSVALILHAPLMFLHFKAPPKRHG
                     AKSLSRLVNIDYIEEQIKKRKEKPVVLPPKIPEAPKKLQEMVKKVEEVKQQIFQRSLP
                     PPPLPAKPKIQDTIARGPEAKLQANLLKDKLEQEKRLLQGKGSFAGKELKLSGPNDTK
                     DLKIGGGGAALTAGSRLSAVGGANGGPKLQGKSGFQVSKDAMPMSIGGDDGDLKIGGG
                     GNIVVVPVGARAQADKSILSPTVGLKDKGALMAQGPGGLTPRAESAAPALAGTGGASA
                     ITIGGSSRQTSISAPPAAAAKEGKRLDSKQMPNPLADAMKTPTSAPALPALPRRMAPK
                     VQKPMFLITGPLADRKALSRVVPEYPEWAREKGVEAAVLIQFTVTPEGAVKDNMLVVR
                     TSGYPPLDALAVKALAQWKFAPLPPDQYRDEIGTITFNFSVQ"
     gene            6300..7259
                     /locus_tag="P5079_03330"
     CDS             6300..7259
                     /locus_tag="P5079_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29050.1"
                     /translation="MKKDARTPLFFRLFGVFLLGLLLGSRAGTLRAEDPQAAAPVSAA
                     VENKEEDSLDVTVTGEAKDEIPVIKTPPALDIPFREVLTLSREGQTDAILDRPVEHMT
                     FEQQVQLVELESRQTVSPLMVQLPKPPFFRIETPRDVSPARWELQVIDQNNQVVGSQQ
                     GTKFTGPFLEWDGFDDGRFGLRVGPSYNAVLTVTDDNEKKQRFFGDSVQLDALQYEED
                     GLLHVEFNNARLYERGSADFSTEMDPLLEAALDIMRRRVGTPFRVIVYEKPELSALAQ
                     KRLDTWKKFLQDNLVIGTDDITLVTMVPRERGPITAVMMLSQP"
     gene            7266..8303
                     /locus_tag="P5079_03335"
     CDS             7266..8303
                     /locus_tag="P5079_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29051.1"
                     /translation="MPNSMELKTVRVKNRRWAAVFLAGGLLFLAPTLPAAEMNRVIHN
                     AMKLYEKGNYDKALKQFIKVLKSDPNNSMAREYMLLCSQKIVETKLGPSTAETVEKEI
                     NVAKQLQEINTAEPSPLTGAGLGIPGADSDLSAKKDVLSPLEAIFAKAAEASPPPPVD
                     DVVDNVPEPDAAPISELPGMEAATTAPAPTPAGLDAAAPGTALAAPAASASAAPAPTS
                     SPSSPTNAQDILRHRQGLADDLRRRHLGTENIVQLEAHRGHLEVNLFLDRLFLPLSDI
                     LRDDAYVILDHVIFEIRRDVKRRVSFKAVDTTSPAVRSSLPQLTSKRCTVVFSYLLYR
                     SLEQKGPTARP"
     gene            8553..9485
                     /locus_tag="P5079_03340"
     CDS             8553..9485
                     /locus_tag="P5079_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY29052.1"
                     /translation="MNDVHRHASPLPPSMTTPLACDFHTHSNYSDGVLTPSQLVRAAR
                     EAGLGVISLTDHDITDGVEEAQAEGRRAGVEVIPGVELSAGVRNAYGVETEMHVLGYF
                     INPRNSHLQQTLAMFRKTRLKRAEHMVAKLAHLGMRLKAEKIDEQSQGKTVGRPHIAR
                     ALVAAGFIGNYQEAFDKYLKEGKPAYVSKALMSPLECIQLIHRAGGLAVVAHPFYGGP
                     RQRQTWDFLIKNGLDGIEAHHSHHPPHTAKHYETLARDYGILVTGGSDFHSAENGPTG
                     NLGDVRMPHRAFAEMRARKEILDAQNLCILQHAS"
     gene            9715..10320
                     /locus_tag="P5079_03345"
     CDS             9715..10320
                     /locus_tag="P5079_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRY29053.1"
                     /translation="MRQNKTPLFDTVLAHAKNRVVSFHTPGHKNGRGIDKRLRDFTGR
                     NMYYMDVTVFPEVDSLHDPQGPIKKAQQLMARAYGVEHSFFLVNGSSVGNMIMLMSSC
                     QPGDSVIISRNAHKSAMSGIVLSGVWPIWVQPKVDQNLDILFDSSPEQIEDALRRYPE
                     AKAVFVTSPTYNGITTDLVRIAEICHSRARCSWWTRRTAPI"
     gene            10290..11168
                     /locus_tag="P5079_03350"
     CDS             10290..11168
                     /locus_tag="P5079_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29054.1"
                     /translation="MVDEAHGPHLKFHKDLPVSAVEAGADLCVQSIHKILSGLSQGSV
                     LHFQSDLVDLARVRRVVSFLQTTSPNYLILSSLDLARRQAVMHGESMLSRVIHHAESA
                     RKRLNQTKNFSCFGRNEIMARGYDLDVTKLTVNVTRTGFSGHEIEDILAKEYRVQVDA
                     SDLFNLIAIMGIGSDKSDVDKLVSSLEDIEKKYHGSAQNWVLQIPTLATEMVMVPRDV
                     FLLYKSKRVPLSKAAGHISAQTLTPYPPGIPVLIPGERITQAIVDYLEDLAEKDIRVV
                     GQEGETLRTIKVVSVR"
     gene            11189..11593
                     /locus_tag="P5079_03355"
     CDS             11189..11593
                     /locus_tag="P5079_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRY29055.1"
                     /translation="MPSRKKKAEKKGGKASGRIKKQPGTQFYERRLTIMRNDLMKLVQ
                     EKQQKEMPDLEVGDEADAATQSSERELLFELSDTERQTLDAIEAALRKIEQGRFGLCE
                     SCHKKILRTRLRAMPQARYCINCQARFERPRF"
     gene            11785..13269
                     /locus_tag="P5079_03360"
     CDS             11785..13269
                     /locus_tag="P5079_03360"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRY29056.1"
                     /translation="MTRFSLSDRFDPKDPLAVFVFQKGQQLFWDGAAKNCPPAVLQTA
                     KEDGYRGKPGGSFIVRPPEGKPAQRLLLLSAGERDELRPPVFRLLGATAAKRASDVGM
                     KTLNVRLPKASGPLEKNIEAFVEGFLLGSYRYLRHKSSPDKTVMPDEIVFLVPEKRRV
                     SLDAAISQGALYAEMTNYARDLINEPPSLKTPEMLSQKASELAGDRIKVEIFKKDVLA
                     KRGMFALLGVNRGSAHPPALVHLRYVPAGKPKARLALVGKGITFDSGGLSLKASGGMK
                     TMKCDMSGAAAVFAVFHALRRLQPRLEVHGITPLTENMPDGNAYKPGDVLRAANGKTI
                     EVLNTDAEGRLVLADALSFAADLKPDAIVDVATLTGAATVALGKAYTALMSNNDVMTE
                     KIRRASEKSGEKIWRLPLEPSYKEHLHSAVADLKNIGNEGEAGTIIGGLFLQEFVGQT
                     PWVHLDIAATAWTNKATPMAPAGATGVMARTLLRYILAEGGAGR"
     gene            13266..13784
                     /locus_tag="P5079_03365"
     CDS             13266..13784
                     /locus_tag="P5079_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRY29057.1"
                     /translation="MTLKNKRIAVLVEKDYQDLEVWFPALRLREEGAEVKFLGTTADE
                     YKGKYGYPLTADGRVEGAAVKDFDAVLVPGGWAPDFLRRHESVLRFVRDMDKAGKVVG
                     AICHAGWVLASAGILKGRTVTAFSAIQDDVRNAGANYVDREVVVDRNLVTSRNPDDLP
                     AFCREIIALLNK"
     gene            13821..14678
                     /locus_tag="P5079_03370"
     CDS             13821..14678
                     /locus_tag="P5079_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY29058.1"
                     /translation="MTKTLTIAHSPDPDDAFMFFGLSQGGVPLPEGFCIKHVLKDIQR
                     LNRDARTGKHHVTAISTAAFPSVADRYWILSVGASIGRKYGPIVVCRPERRRRFESAD
                     WSSLRVAIPGEQTTAYLLLRLYRKKFIPVVTPFDKVFQAVSSGKADGGLVIHEGQLTY
                     KRQGFFRAVDLGAWWFRRTGLPIPLGLDVVRKDLGRRTAEELAGALVRSIRYAYRHKA
                     AALRYSLKFGRGIDARVGEKFVRNYVNRDTLDMGPEGERAIRKLFSEARRSGLLPERV
                     RPEIIRPVY"
     gene            complement(14672..14998)
                     /locus_tag="P5079_03375"
     CDS             complement(14672..14998)
                     /locus_tag="P5079_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRY29059.1"
                     /translation="MEKKTFPYETIIFVCNNARPAAGGAVPPRISCAGEGRAGADILE
                     RLKAEVKERRLKGRVRVSKAGCLDLCEQGPNVMVFEGRGERFFYGGVVVNDVPELVRR
                     HFPENQ"
     gene            complement(15002..16120)
                     /gene="mqnC"
                     /locus_tag="P5079_03380"
     CDS             complement(15002..16120)
                     /gene="mqnC"
                     /locus_tag="P5079_03380"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRY29060.1"
                     /translation="MRTANGWNRIEKKVMEGKRLSPAEGVSLLSEKYPFLELGALADW
                     KRFRLFPERQVTFVIDANPNYTNVCDTGCHFCAFFRRPGHPEAYTLTVEQVLEKIGAS
                     AARGATTVLLQGGHNRGLPLEYYEELLRETARRYPGMTPHFFSAPEIRTMAEVSGRPV
                     PEVLKILWNAGQRTLPGGGAEVLSRRVRREISPKKGGPEDWLEVHRVAHRLGYRSTAT
                     LMFGHVETPGDVVEHWDRLRNLQDETGGFTAFIPWSFKPGNTALEKKGLRPAGAVRYL
                     RILAASRLYLDNFPHVQASWFSEGRKTGQAALRFGADDFGGTVFDENVHAAAGHRAAA
                     AAEEVAALIREAGFVPVQRDTLYKTLKIFPGPGRRARR"
     gene            complement(16117..16869)
                     /locus_tag="P5079_03385"
     CDS             complement(16117..16869)
                     /locus_tag="P5079_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29061.1"
                     /translation="MRSRAPLRVGRIPFLNAAPFFAGNAFRGLVTVSAAPRRLGRLAA
                     AGRVDTGAFSLADVWRLAKTFEPMGNYCIASKRRAASVLLFSRRPVQELKDCSLGVTD
                     QTSTSVLLLRLLLRSYYKVRVRVRKGFRPDDAARLLIGDAALRANRRRTGFRYVYDLG
                     EEWYRWKKLPFVFARWAVRKNLPSESKKDLSRRLGRALRCGTDGCRFRRSPLPPAATT
                     AYLRLLSYRFSKKERDSERLFRRLLRGAGLLR"
     gene            complement(16866..>17937)
                     /locus_tag="P5079_03390"
     CDS             complement(16866..>17937)
                     /locus_tag="P5079_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRY29062.1"
                     /translation="LRSLGNKVESGTRLSLDDGRRLFASDDLLGIGRLADHVRRKRHR
                     DRTTFVVNRQINPTNICSLSCRFCCFSVKDGAPGAYVLGIRDILSRLSPDLREVHIVG
                     GLYEGWDFEDYLNIVREVHRAAPAVSIKAYTAVEIDFFARREKTTAGEILRRLKEAGV
                     VCLPGGGAEVFSERVRKILFPRKIGRDRWLDIHRTAHRMGLRSNATLLYGHIETVEER
                     LDHLMKLRSLQDDTGGFLSFIPLPFQPGDSGLVDRPASAMDDLKTVAVSRLLLDNFDH
                     IKSYWVAVGEDLCSVALHFGADDVDGTIREERVMHAARARTPEGLTRDRIVRLVREAG
                     FVPVERDGLYRNRREVRGGRSA"
BASE COUNT         3694 a         6146 c         5098 g         2999 t
ORIGIN      
        1 ctggcggtga tgggctcgtg ggcggtgcgg aatcctttga tgtcgctgtc cacgccgttg
       61 ctgattctcg ccatccccat tttcgacatg atctacacca ccgtctcccg catccgcaac
      121 gggcgggtgc gcaacgtgcg cgaatggctg gagttcgtcg gccgcgacca tttccatcac
      181 cgcctcatgc atctgggaat ggaagaacgt caaaccgtgg ggttcatcct gctggtcaac
      241 ctatgcctgg gactcagttc catcaccgtc cgctacaccg ccacccggct gagcgccctc
      301 ctcatgctct ttcaggcgtt tttgatcttc atgatcatca cggccctgat gctcctgggg
      361 cgggagatca ccccgcatcc cctcaaagga aaaacgtgac ccgatgaacg gcctcttctt
      421 ccgatcctgc tcgaaaaaag caagaaccgt cgcggggatc gtcgcgctgg ccctctccct
      481 gccggccccg gcggtgcagg gggccaaacc gccggcggcg gacgccccca ccgcctacac
      541 cctggagttc accccccgga acgtccaacg cacgctcgac gactttctga agttcatcca
      601 agccaccgcg ccggccaccg acggcatccg cgcccaatac ctggccgccg aactttatct
      661 gcagcgcggc gagttccgcc gggccgcgga gatcttccaa aaactcgccg cggtcacctt
      721 cgacgacaaa tatttcaacg tcagcgtcct tgaaaaactg gcggactgtt atttccgcca
      781 gggccttttc caacaggccg ccgattccta catgaccgtc cgcaacagca acgtgaaggc
      841 catcctgccg gaggcgctcc tggggttgtc ggtctcggcg ctggccctcg gcaaccgcga
      901 ggaggcctac ctgcgcatcc aggaactcct ggccttccat cccgcctatg ccaaccacgt
      961 cccgctcatc ctgcccatcg gtctcgtcca ctgggagaac ggcaagttcc aggaagcgct
     1021 cgaccttttc cagcgcgatc cggacaaccc cgccagcctc tatttcgccg gactctgcca
     1081 gcggtctttg gacaagcccg tggacgccat gggcacgttc aagaaggtcg tgcagaacca
     1141 ccggaaaacc gtctggggca cgcgcgccaa attcgaactg gccgaaacgt tctaccagaa
     1201 aaaggattat ccgctggctt cccgcacctt cgaccaaatc taccgcggcg agagggacga
     1261 cgcctgggaa accctcgccc tctaccgcct ggcctgcagc gacatgcaca tgcgccgttt
     1321 caaggacgcg gaaaccaaac tttggcccct gcagaagaag aaaaaagacc accgcctcca
     1381 ccccaacgtc aactacctcc tcacggagtc gctggccgaa cagaacaaga tcgagaagat
     1441 ggtgcccctg ctgaaagcgg agatcaaagg caagggccgc acgccggaca atatctaccg
     1501 cctcatctgg tcgctggcgg ccatcggcga ttacgcggag gtcatccgcc agtccaacga
     1561 attcctcagc acctcctggg acatggagct cacgcccaaa accctggtgg cccagggcta
     1621 cgcctacgac aagctgggcc gcacgccgga atccgcggcg agctaccagc tggtggcgga
     1681 gaatttttcc aacacgccct acgccgcccg ggccctccag ctcaccgcca tgacctattt
     1741 ccgttccaag cagcacggac tgatcgtcac ccaggtcaac aaccaatgga acgccgtccc
     1801 gctggaaatc cgccagaaaa acccggaaac gcttttctgg atcgcggaag cccacctcca
     1861 actggagaac ggggccgagg cgcgggagca ttacaagaaa ttcctcgatt tcgcgccgcc
     1921 cgaccatccg ctggggaccc aggccctcat gggagaggcc gtctcttacg ccgtggacaa
     1981 ggatttcccg acggcgatcc tcaccctcca aaaaacgctt cagtccgccc aggagaaggg
     2041 caacaagccc ctcatggccg cctccatgct ggagatgggc aacgtgttct tcaacgccaa
     2101 ggattacgag aacgccgcct cgtcttaccg cagtttccgg gaggtcgatc ccaagcacgc
     2161 acaggcggcc ttctcgttgt atcaggaggg gttggcgctg caccgggcgg aatactacag
     2221 cgacgccatc gccgcgtggg acaaactcgt caaaacccac cccaaggccc cgcaggcctc
     2281 ggaagccctc ttccgcaagg ccaagaccct tttcgatacg ggcaactatc ccggcgcggt
     2341 gaaggactac gaaaaactca tcggcaatta ccccaagagc gagttcgcca aggacgcccg
     2401 ccttcagatc ggccagtgct tttacaacgc gggcgatttc gccaaggcca tcgtccatta
     2461 caccgacttt ctcaaccgct tccccgacga cccgctcgct cccagcgtgc tgcagctcct
     2521 gcagacctcc tactatcagg ccaagaaaac gccggacgag atcgagaaac tcacccgcaa
     2581 ccagcccaag agcgccatct tggccgacat ctattgggag gaaggggcca aactttacaa
     2641 cgagaagagc tacgacaagg cgcgggaata cttccagaag atcctctcgg agttcccgtc
     2701 ctcctcgctc gccccgcagg ccgcgttcta ccgggcggag tcgctctacc tccaggaaaa
     2761 atatttcgag tccatcccgg cctatgagaa cttcatccgc tattaccccg aagacgccca
     2821 gcggagcctg gccatgttcc atctctccgt cggccttttc aatcagaagg aatacgccga
     2881 aagcgccaag gcgttcggcg acttcgccgc ccagttcccc gacgatccca tggccaagaa
     2941 cgccaacttg aacgtggccg tctgctacgc caaagcgcag gacgtcaaca acgcggtggc
     3001 cgcctacgag cgctacatcg cgctctatcc cgacggcgag gacgtgggcg ccacgtatct
     3061 gcagatggga cagttgctgg aaaagttcgg ccaggaggcc cgcgccgccg acgtgtacaa
     3121 acgcgtgccg aaaaacctgc cggaatacgc cgaggccctc ttcaacgccg gccgctgcta
     3181 ccgcgccctc aatcaattgg cgggggaaca acaggcctac gaggacctgc gcctcttcgc
     3241 gcccaagaac gacgcctacc gcaccgccgg gctcctgcag ttggcggaaa tccatttgac
     3301 gaagaacgag acggcgaaag ccgcggagat ctaccgcgac gtcgtcgcca acgccgccga
     3361 cgaccagagc cgggccatgg cggaagaacg cctcaaaacc ctcggaccct agcgccgggg
     3421 cggaaacgac tttaacgact tcaagggagg attcatgcac gaactcacgt ttatcgagct
     3481 gatgaagatc agtctggtga tgccggtgct cctggtgctg tccatcgtgt tggtgatggt
     3541 attcctggaa cgctggatat tcttcacgcg catggcgcgg gtggacgctt ccctgttcaa
     3601 acgggtcaag caggctgtgc gggagaaaaa actgaaagag gcccaacaga tcgcctccgg
     3661 cggacggggg ctcctcgccc aggccctgga agcggagttg gacgccgcgc agaccgggtc
     3721 gcgggaggaa atcgacaccg tgctcaccct ctattaccag cgcgtgcagg ccctgctcag
     3781 ccggcggctg ggcatcttcg gcacgctcag cttcatctcg ccgctgctgg gcctgttggg
     3841 cacggtgctc ggcgtcatgc gggctttccg tgacctggcc ctctccggtt cgggcgggcc
     3901 caccatcgtg gcggccggca tctccgaggc tctcatcgcc accgcggcgg gcatcgccgt
     3961 ggccgtgagc gcggccctga tctacaacta cttcaatttc cgtctcaagt acgtgctcaa
     4021 cagcctgaac ctgttcggcc aggaaatcct gctcctggtg gtcagcggaa aagaaatcta
     4081 accgcggcct tcgtaacccg gaaggacaaa agaaaaagat ggacgatccg tctcaaagcg
     4141 gagaggtcga ggaaaccgag aagatcaacg tcgttccgct cgcggacctg acgctggtgc
     4201 tcttgatcat cctgatggtg ctctctccca tgatcaccca atccatgatc aacgtcgcca
     4261 cgccggcggt gaaagcggag aaggacatcg agcagaaacc ccaggaggag aagaaaccgg
     4321 tggaacccct gctcatcgcc ctcgcggaat ccggatacac gctcaacaac gtaccggcgg
     4381 acagtctcga ccagctcatg gccgccctcc gggcgcggct ggaggagacc ccggaccgtc
     4441 cggtcctcgt caccgccgac cagcagatca ccgtgggcgc ggtggtggag gtgctcgacc
     4501 tggccaaaca gaacggcgcg caaaaagtgt ccctgctcaa acgggccggc gtcgaagaaa
     4561 gcgcggagaa gaccgccccg tgaaaaagct cgcccgcagc ttcgaagacg cccccatcgc
     4621 cggcatcaac atcgtgccgg tgatcgacct ctgcctggtg ctcctggtca tcctcctcat
     4681 cctgtcgccc ctgatggacg cgcccaacct caccatcaaa ctgccggaag ccaagacgaa
     4741 ggaggagaag gagaacatca tctccctctc cgtcacgccc gacggaaaaa tggcgctcaa
     4801 cacggacctc atcgaggcgg gcgacatccc caagctcctc cccgtgctcc tggcggagca
     4861 gggggaaaac acgccggtgg tcatccgcgc cgacaagaac gtgacctacg gccggctcac
     4921 cgatcttttg aagaccgtca aaaccgccgg ggccaaacgg atctccctcg gcacggaaca
     4981 gcccaaaggg gcggcccaat gatgcgctcc tggaacctcc tctacgaacg ccagccgctc
     5041 cagctcgacg tcctcctcct gtcggtggcg ctgatcctcc acgcgccgct gatgttcctc
     5101 catttcaagg cgccgcccaa acggcacggg gccaaatccc tttcgcggct ggtcaacatc
     5161 gactacatcg aggaacagat caagaaacgg aaggaaaaac ccgttgtcct ccctccgaaa
     5221 atccccgaag ccccgaaaaa actccaggag atggtgaaga aggtggaaga ggtgaaacag
     5281 cagatcttcc agcgttccct gccgccaccg cccctgccgg cgaaaccgaa gatccaggac
     5341 accatcgcac gcggaccgga ggccaagttg caggcgaacc tcctcaaaga caaactggag
     5401 caggaaaaac gcctcctgca ggggaagggc tccttcgccg ggaaagaact aaaactctcc
     5461 ggccccaacg acaccaagga cctaaagatc ggcgggggcg gagccgctct tacggccggc
     5521 tcccggctct ccgccgtcgg cggggccaac ggcggcccga aactgcaggg gaaatcgggg
     5581 ttccaggttt ccaaggacgc catgccgatg tccatcggcg gagacgacgg ggacttgaag
     5641 atcggcggcg gggggaacat cgtcgtggtg cccgtcggcg cccgggccca agcggacaaa
     5701 agcattctct ctcccacggt gggactgaaa gacaaaggcg ccctgatggc gcaaggaccg
     5761 ggcggattga cgccgcgggc cgaatccgcc gcgcccgcgt tggcggggac gggcggagct
     5821 tccgccatca ccatcggcgg ctcgtcccgg caaacctcga tctccgcgcc gcccgcggcc
     5881 gcggccaagg aggggaaacg gctggactcc aaacagatgc ccaacccctt ggccgacgcg
     5941 atgaagaccc cgacgtcggc ccccgccttg ccggccctgc cgcggcgcat ggcgccgaaa
     6001 gtgcagaaac ccatgttcct catcaccggt ccgctcgccg acaggaaggc cctttcgcgc
     6061 gtggtccccg aatatcccga atgggcgcgt gaaaaaggcg tcgaggcggc ggtgctgatc
     6121 caattcaccg tcacgccgga aggggccgtg aaagacaata tgctcgtcgt ccgaacatcg
     6181 gggtacccgc cgctggacgc gttggccgtc aaagccctgg cgcagtggaa attcgcgcct
     6241 cttccgccgg accagtatcg cgacgagatc ggcaccatca ctttcaactt ttcggtccaa
     6301 tgaaaaaaga cgcgcggact ccgctgttct tccgactctt cggcgttttc ctcctcggcc
     6361 tgctcctcgg gtcccgggcc gggaccctgc gcgccgaaga ccctcaggcg gccgcgccgg
     6421 tttccgccgc ggtggaaaac aaggaggagg actccctcga cgtcaccgtc accggcgaag
     6481 ccaaagacga gatccccgtc atcaaaacgc cgccggcgct ggatatcccg ttccgggagg
     6541 tgctcacgct ttcgcgcgaa ggccaaacgg acgccatcct ggaccgcccc gttgaacaca
     6601 tgactttcga acaacaggtt cagctcgtcg agctcgaatc ccggcagacc gtttccccgc
     6661 tcatggtcca gctgccaaag ccgccgtttt tccggatcga aaccccgagg gacgtcagtc
     6721 ccgcccgctg ggaactccag gtgatcgacc aaaacaacca ggtggtcggc tcccagcagg
     6781 ggaccaaatt caccgggccg ttcctcgagt gggacgggtt cgacgacggc cgcttcggcc
     6841 tgcgggtcgg accttcctat aacgccgtcc tcaccgtgac cgacgacaat gaaaagaaac
     6901 aacgtttctt cggcgattcg gtccaactgg acgccctgca gtatgaagag gacggcctgc
     6961 tccatgtgga attcaacaac gcgcggctct atgagcgggg ttccgccgat ttctcgacgg
     7021 agatggaccc tcttctggaa gcggcgctgg acatcatgcg ccgaagggta gggacgccgt
     7081 tccgcgtcat cgtttacgaa aaaccggagc tctccgcgct cgcccagaag cgtctcgaca
     7141 catggaaaaa gttccttcag gacaacctcg tcatcggaac ggacgacatc accctggtca
     7201 ccatggtccc gcgcgagagg ggcccgatca ccgcggtcat gatgctttct cagccgtaaa
     7261 aacatatgcc gaattcgatg gaactcaaga ccgttcgcgt caaaaatcgc cggtgggccg
     7321 ccgtcttcct cgccggaggg ctcctgttcc ttgccccgac gctccccgcg gccgagatga
     7381 accgggtcat ccacaacgcc atgaagcttt acgaaaaggg gaattacgac aaggccctga
     7441 aacagttcat caaagtattg aaaagcgatc ccaacaactc catggcgcgg gaatacatgc
     7501 tcctctgcag ccagaagatc gtcgaaacca aattggggcc gtccaccgcg gaaaccgtcg
     7561 aaaaggaaat caacgtcgcc aagcagctcc aggaaatcaa cacggccgaa ccttcgccgc
     7621 tgaccggcgc cgggctgggg atcccgggcg cggacagcga cctgtcggca aaaaaagacg
     7681 ttctgtctcc cctggaagcc atttttgcca aagccgcgga agcctcgccg ccgccgcccg
     7741 tggacgatgt cgtggacaac gttccggaac cggacgccgc ccccatctcc gaactccccg
     7801 gcatggaagc cgccacgacc gcccccgccc cgacgccggc cgggctcgac gcggcggccc
     7861 cgggaacggc cctcgcggcg ccggcggcct cggcgtcggc cgcaccggcc cccacaagct
     7921 ctccgtcttc tccgaccaac gcccaagaca tcctccggca ccgacagggg ctcgccgacg
     7981 atctccgccg gcgtcacctg ggaacggaaa acatcgtcca actggaagcc caccgcggcc
     8041 acttggaagt gaaccttttc ctcgaccgcc tcttccttcc cctgtcggac atcctgcggg
     8101 acgatgccta cgtcatcctg gatcacgtca tcttcgaaat ccgccgggac gtcaaacgcc
     8161 gggtctcctt caaggccgtg gacaccacct cgccggccgt ccgcagttct ctgccccaac
     8221 tcacctcgaa acgctgcacc gtggtttttt cctaccttct ctaccgctcc ctcgaacaaa
     8281 aagggccgac cgcccggccg taattttcag aagttctgct gaaaaattcc cgatttccgg
     8341 cgccgaccga aaatccccgg aggacacggg actttttttc gcccccgacg gccgcggcgt
     8401 tccgtcggtg atttgacaga aaaaaataaa atgtagtaaa gtgcttacgg cttccgcgca
     8461 ttgcttaaaa atttttctcg ctgactcgcc gcaacgccgg aaaaatattc ttcacgcgat
     8521 atccccgcca aatcgagatc ttccgaggtt ttatgaatga cgtccatcgc cacgcgtcgc
     8581 ctctcccgcc gtccatgacc acccccctcg cctgtgattt ccacacgcat tccaactact
     8641 ccgacggcgt cctcacgccc tcccagctgg tccgggcggc gcgggaggcc gggctgggcg
     8701 tcatctccct caccgaccac gacatcacgg acggcgtcga agaagcccag gcggaaggcc
     8761 gccgcgcggg cgtggaagtg atccccggcg tggagctctc cgccggcgtc cgcaacgcct
     8821 acggcgtgga gacggaaatg cacgtgctcg gttatttcat caaccccagg aacagccatc
     8881 tccagcagac cctcgccatg ttccgaaaaa cccgcctcaa acgggccgaa cacatggtcg
     8941 ccaaactggc gcacttgggg atgcggctca aagccgaaaa gatcgatgaa caatcccagg
     9001 ggaaaaccgt cggccggccc cacatcgccc gggcgctggt ggccgcgggg ttcatcggca
     9061 attaccagga ggccttcgac aagtacttaa aggaagggaa acccgcctat gtctccaaag
     9121 ccctcatgtc gcccctggaa tgcatccagc tgatccaccg ggccggcggg ctggcggtgg
     9181 tggcccaccc cttctacggc ggaccgcgac aaaggcagac ctgggacttc ctgataaaaa
     9241 acgggctcga cggtatcgag gcccaccact cgcatcaccc gccccacacg gcgaaacatt
     9301 acgaaacgct ggcccgcgat tacggcatcc tcgtcaccgg cggctccgat ttccattccg
     9361 ccgaaaacgg ccccacgggg aacctcggcg acgtccgcat gccccatcgc gccttcgccg
     9421 agatgcgcgc ccggaaagag attctggacg cgcaaaacct ctgcatcctc cagcacgcct
     9481 cctgaccggc cccgttccgc ggcggccttt ctttccgcgg cgcgccccct tgaaaacggc
     9541 gccgccgtcc gacggaacaa aattccttga cactcccacg gaagcttctt aatatttgaa
     9601 ctcgccgtag gaaattcgcc gagcgcggcc cctctccgtt tatcactgtt cggaccgcct
     9661 tttcgtttcg gaagttgcta aactttcaca ttcaccgacg tcacagatga ggtcatgaga
     9721 cagaacaaaa ccccgctttt cgacaccgtc ctcgcccacg ccaaaaaccg cgtggtctcc
     9781 tttcacacgc ccggccacaa gaacgggcgc ggcatcgaca agcgcctgcg cgatttcacc
     9841 ggccgcaaca tgtattacat ggacgtcacc gttttccccg aagtcgattc ccttcacgat
     9901 ccccagggac ccatcaaaaa ggcccagcag ttgatggccc gggcctacgg cgtggaacac
     9961 tccttcttcc tggtcaacgg ctcctccgtc ggcaacatga tcatgctgat gtcctcctgc
    10021 cagccgggag attccgtcat catttcgcgc aacgcgcaca aatccgccat gtcgggcatc
    10081 gtcctctccg gcgtctggcc catctgggtg cagcccaagg tggaccagaa cctggacatc
    10141 ctcttcgatt cgtccccgga acagatcgag gacgccctgc gccgctatcc cgaggcgaaa
    10201 gcggtgttcg tcaccagccc cacctacaac ggcatcacca ccgacctggt gcgcatcgcc
    10261 gagatctgcc actcgcgggc aaggtgctca tggtggacga ggcgcacggc ccccatttga
    10321 agttccacaa ggacctgccc gtctcggcgg tggaggccgg ggcggacctg tgcgtgcagt
    10381 ccatccacaa aatcctctcc ggactgtccc aggggtccgt cctccacttt caatccgacc
    10441 tggtggacct ggcccgcgtg cggcgcgtcg tctccttcct gcagaccacg agccccaact
    10501 atctgatcct ctcgagcctc gacctggccc gccgccaggc cgtgatgcac ggggaatcca
    10561 tgctctcccg cgtcatccat cacgccgaaa gcgcccgaaa acgcctcaac cagacgaaaa
    10621 atttctcctg cttcggccgc aacgagatca tggcgcgcgg ctacgacctc gacgtcacca
    10681 aactgaccgt gaacgtcacg cgcacgggtt tctccggcca cgagatcgag gacatcctgg
    10741 ccaaggaata ccgggtccag gtggacgcct ccgatctctt caacctgatc gccatcatgg
    10801 gcatcggctc ggacaagtcg gacgtggaca aactggtctc ctccctggag gacatcgaga
    10861 agaaatacca cggcagcgcg cagaactggg tccttcagat ccccacgctc gccaccgaaa
    10921 tggtgatggt gccccgcgac gttttccttc tttacaaatc gaaacgcgtg cctctttcca
    10981 aagccgccgg gcacatttcc gcccaaaccc tgacgcccta cccgcccggc atccccgttc
    11041 tcatcccggg ggaacgcatc acgcaggcca tcgtggacta cctcgaggac ctggcggaaa
    11101 aggacatccg cgtcgtcggc caggagggag aaaccctccg caccattaaa gtggtttccg
    11161 tacgataacc catcaaagga gaacaaccat gccttcgcga aaaaagaaag ccgaaaaaaa
    11221 agggggaaag gcatccggcc gcatcaaaaa acagccgggc acccaatttt acgaacgccg
    11281 cctgaccatc atgcggaacg atctcatgaa gctcgttcag gaaaaacagc aaaaagagat
    11341 gcccgacctc gaggtcgggg acgaggcgga cgccgccacg caatcatcgg aacgggaact
    11401 gctctttgaa ctttccgaca ccgaacgcca gacgctggat gccatcgagg ccgccctccg
    11461 aaagatcgaa caggggcggt tcgggctctg cgaatcctgc cacaaaaaga tcctccgcac
    11521 ccggctgcgg gccatgccgc aggcccggta ctgcatcaac tgccaggccc ggttcgaacg
    11581 gcctcgcttt taattcaccg ggccctcttt ttcgcgcggc cgccgcaccg ctttccgcct
    11641 ccgcccacgc ggcggctatt cttgaacttg ttataatgaa cggatgcggc cgatccgacc
    11701 ggtgaccccg gtccggacat gaaagaattt ccaacctcga ggaacgggac cggttcccgc
    11761 ttcctcctca aaaggatgcc ctctatgaca cgcttttcac tctccgaccg ttttgatccc
    11821 aaggacccct tggcggtgtt cgtttttcaa aaagggcagc agctcttctg ggacggggcg
    11881 gccaagaact gccctccggc cgtattacaa acggccaagg aggacggtta ccgggggaaa
    11941 ccgggaggat cgttcatcgt ccggccgccg gaggggaaac ccgcccagcg gttgctcctt
    12001 ctgtccgccg gcgagcggga cgaacttcgg cctccggttt tccgtcttct gggggccacc
    12061 gccgccaaac gcgcttccga tgtcgggatg aagacgctca acgtccggct tccgaaggcc
    12121 tcgggccctt tggaaaaaaa catcgaggcc tttgtggaag gttttcttct ggggagctac
    12181 cggtatctgc gccacaaatc gtcgccggac aaaacggtca tgcccgacga gatcgtcttc
    12241 cttgttccgg aaaaaagacg ggtctcactg gacgcggcga tctcccaagg cgcgctttac
    12301 gcggaaatga ccaattatgc ccgggatctc atcaacgaac ctcccagcct gaaaactccg
    12361 gagatgctgt cccaaaaagc atccgagctg gccggggacc ggataaaagt cgaaatcttt
    12421 aaaaaagacg tcctggccaa gcgggggatg ttcgccctgc tgggcgtcaa ccgtggcagc
    12481 gcccacccgc cggcgctcgt acacctgcga tacgtccccg ccggcaaacc caaggcccgc
    12541 ctcgccctgg tcggcaaagg catcaccttc gattccggcg gcctttccct gaaagcatcc
    12601 ggtggcatga aaaccatgaa atgcgacatg tccggcgccg ccgcggtctt cgccgttttc
    12661 cacgccctcc gtcgccttca accccggctg gaggtacacg gcatcacccc gctcacggag
    12721 aacatgccgg acggcaacgc ctacaaaccc ggggacgtcc ttcgggcggc caacggcaag
    12781 accatcgagg tattgaacac ggacgccgag ggacggctcg tcctggccga cgccctttct
    12841 ttcgcggcgg acctgaaacc ggacgccatc gtggacgtgg ccacgctgac cggtgcggcc
    12901 accgtggccc tgggaaaagc ttatacggcc ctgatgtcca acaacgacgt tatgacggaa
    12961 aagatccggc gggcctcgga gaaatccggc gaaaaaatct ggcgtctgcc cctggaaccc
    13021 tcctataagg aacacctcca cagtgcggtg gcggacttaa agaacatcgg caacgagggg
    13081 gaggcgggga cgatcatcgg cggcctcttc ctgcaggaat tcgtgggcca gacaccctgg
    13141 gtccacctgg acatcgccgc cacggcttgg accaacaaag ccacgccgat ggccccggcg
    13201 ggcgcgaccg gcgtgatggc gcgaacgctt ctgcgctaca tcctggccga gggaggagcg
    13261 ggccgatgac cttgaaaaac aagcggatcg ccgtcctggt ggagaaggat tatcaggacc
    13321 tggaagtgtg gttcccggcc ctgcgcctgc gcgaggaggg ggccgaggtg aaatttctcg
    13381 gcaccacggc ggatgagtac aaagggaaat acggttaccc gctcacggcc gacgggcgcg
    13441 tcgaaggcgc cgcggtcaag gatttcgacg ccgtcctcgt gccgggcgga tgggcgcccg
    13501 atttcctgcg acgccacgag tcggtcctcc gtttcgtgcg ggacatggac aaggccggaa
    13561 aagtcgtggg cgccatctgt cacgccggct gggtgcttgc ctccgcgggc atcctcaagg
    13621 gccggaccgt caccgccttc tccgccattc aggacgacgt gcggaacgcc ggagccaact
    13681 acgtggacag ggaagtggtg gtggaccgga acctcgtcac gtcacggaac ccggacgacc
    13741 ttccggcgtt ttgccgcgaa atcatcgcgt tgttgaataa atagtcaccg cccgcgcccg
    13801 aaccgctctc ccgcttcccc gtgacaaaaa cgctcaccat cgcccacagt cccgaccccg
    13861 atgacgcctt catgttcttc ggcctcagcc agggcggcgt ccctctcccc gaggggttct
    13921 gcatcaaaca cgttctgaag gacatccaac gcctcaaccg ggacgcccgc accggaaaac
    13981 atcacgtcac cgccatctcc accgccgcct tcccgtcggt ggcggaccgc tactggatcc
    14041 tttcggtcgg cgcttccatc ggccggaaat acgggcccat cgtcgtctgc cggccggaac
    14101 gccggcgtcg ctttgaatcg gcggattggt cttcgctgcg cgtcgccatc cccggggaac
    14161 agaccaccgc ctacctgctg ttgcgccttt accggaaaaa gttcatcccg gtcgtcacgc
    14221 cgttcgacaa ggttttccag gccgtttcct ccggaaaagc cgacggcggg ctggtcatcc
    14281 acgaaggcca gctcacgtac aaacgacagg gatttttccg ggcggtggat ctgggggcgt
    14341 ggtggttccg tcgaacgggg cttcccatcc ctctcgggct ggacgtcgtc cgcaaggatc
    14401 tggggcggcg gacggccgaa gaactggcgg gcgcgctcgt gcgcagcatc cgttacgcct
    14461 accgccacaa agcggccgcc ctccgctatt cgctgaagtt cgggcggggg atcgacgcac
    14521 gggtgggaga aaaattcgtc cgaaactacg tcaaccgcga cacgttggac atggggccgg
    14581 agggggaacg ggcgatacga aaactttttt ccgaagcgcg ccgaagcggc cttctccccg
    14641 aacgggtccg gccggagatc atccgcccgg tctactgatt ttcggggaag tgcctccgca
    14701 ccagttccgg gacatcgttc accaccacac cgccataaaa gaacctctcg ccgcgacctt
    14761 caaaaaccat cacgttcggc ccctgttcgc agaggtccag gcagccggcc ttggacaccc
    14821 gcacacggcc cttgagacgc cgttccttga cttccgcctt caatcgctcg aggatgtccg
    14881 cgccggcgcg gccttctccg gcgcaggaga tcctcggcgg cacggccccg cccgccgccg
    14941 gccgcgcgtt gttgcagaca aaaatgatgg tttcataggg gaaagttttc ttttccaccg
    15001 gtcacctcct cgcccgacgt ccgggcccgg gaaatatttt caaggttttg tagagcgtgt
    15061 cgcgttggac agggacgaaa cccgcttccc ggatgagcgc cgccacctcc tcggccgcgg
    15121 ccgcggcgcg gtgcccggcg gcggcgtgca cgttctcgtc gaacaccgtg ccgccgaaat
    15181 cgtccgcgcc gaaacgaagg gccgcctgcc ccgtcttccg gccttccgag aaccacgacg
    15241 cctgcacgtg cgggaaattg tccagataca accgggaagc cgccaatatg cgcaggtacc
    15301 gcaccgcccc ggccggacgc agtccctttt tctccaacgc cgtgttcccc ggcttgaagc
    15361 tccaggggat gaacgccgtg aacccgcccg tctcgtcctg caagttccgc agacggtccc
    15421 aatgctccac cacgtcgccg ggggtttcca catggccgaa catcagggtg gcggtgcttc
    15481 ggtaccccag ccgatgggcc acgcggtgga cctccagcca gtcttccggc ccgccctttt
    15541 tgggcgagat ctcccggcgg actcggcggg acaggacctc cgccccgccc ccggggaggg
    15601 tgcgctggcc ggcgttccac aaaatcttca acacctccgg gaccggccgg cccgagacct
    15661 cggccatggt cctgatttca ggagccgaaa aaaagtgcgg ggtcatcccc ggataccgcc
    15721 gcgccgtctc ccgtaaaagt tcttcgtaat actcgagggg aagtccccgg ttgtgccctc
    15781 cctgcaaaag aacggtcgtc gcgccccgcg ccgccgacgc ccctattttc tccaggacct
    15841 gttccaccgt cagcgtgtac gcctccggat ggcccggccg gcggaaaaac gcgcaaaaat
    15901 gacatcccgt gtcgcagacg ttggtgtagt tgggattggc gtcgatcacg aaagtcacct
    15961 gccgttcggg aaaaagccgg aagcgtttcc aatccgccag ggcccccaac tccaggaaag
    16021 gatacttttc cgacaagagg gaaaccccct ccgccggaga gagccgtttt ccctccatga
    16081 cttttttctc gatccggttc cagccgttcg ccgttctcat cggagcagtc ccgccccgcg
    16141 cagcaagcgc cggaacagtc tttcagaatc tcgttctttc ttggaaaaac ggtagcttaa
    16201 caaccgcaag taggccgtcg tggcggccgg ggggaggggg gaccggcgaa agcggcatcc
    16261 gtccgtcccg caccgcaaag ccctgcccaa acgacgggac aagtccttct tgctttccga
    16321 cggcagattt ttccggaccg cccaacgcgc gaaaacgaag gggagtttct tccagcgata
    16381 ccattcctcg cccagatcat agacatatcg gaagcccgtc cggcgacggt tcgcgcgcag
    16441 ggccgcatcg ccgatcagca gccgcgccgc gtcgtccggc cgaaaccctt tccggaccct
    16501 cacccggacc ttgtaatagc tccgcaagag gagtctcaga aggaggacgg aggtggacgt
    16561 ttgatcggtc acgccgaggg agcaatcctt cagttcctga acgggtcgtc gggagaacag
    16621 cagaacgctg gcggctctcc gtttggacgc gatgcagtaa ttccccatcg gttcgaacgt
    16681 cttcgccagc cgccacacgt ccgccaggga gaaggccccg gtgtccaccc ggccggcggc
    16741 cgccagccgc cccaatcggc gcggcgcggc ggaaaccgtg accaggcccc gaaaagcgtt
    16801 cccggcgaaa aaaggagcgg cgttcaaaaa ggggatacgg cccacgcgga gaggcgcacg
    16861 ggacctcatg cggagcgtcc tcctcggacc tcacgccgat tccggtaaag gccgtcgcgc
    16921 tccacgggca cgaaccccgc ctcccgcacc agccggacaa tccgatcgcg cgtcagtcct
    16981 tcgggggtac gagcccgcgc ggcatgcatc acgcgctctt cccgaatggt gccgtccacg
    17041 tcgtccgcgc cgaaatgcag cgccaccgaa caaaggtcct ctcccaccgc cacccaatag
    17101 gacttgatgt ggtcgaaatt gtccagcagg agccgcgaca ccgccaccgt cttcagatcg
    17161 tccatcgcgg aagccggccg gtcgacgagg cccgaatccc ccggttggaa cgggaggggg
    17221 ataaacgaaa gaaacccgcc ggtgtcgtcc tgcaaagacc ttaacttcat caggtggtcg
    17281 agccgttcct ccacggtctc gatgtgcccg tacagcagcg tggcgttgga gcggagtccc
    17341 atgcggtggg ccgtccgatg gatgtccagc cagcgatccc ggccgatctt gcgcggaaag
    17401 aggatcttgc ggacccgttc gctgaacacc tccgcgcctc cgcccggcag gcacaccacg
    17461 cccgcctcct tcaaccgccg taaaatttcc ccggcggtcg ttttctcccg gcgcgcaaaa
    17521 aaatcgatct ccaccgcggt gtaggccttg atggacacgg cgggagcggc ccggtgcacc
    17581 tcccgaacga tgttcaggta atcctcgaaa tcccatccct cataaaggcc tccgacgatg
    17641 tggacttccc gcaggtccgg cgacaggcgc gacaggatgt cccggatgcc gaggacgtag
    17701 gcgccgggag cgccgtcttt cacggaaaaa cagcagaaac ggcaggagag ggaacagatg
    17761 ttggtggggt tgatctgccg gttgacaacg aaggtggtcc ggtcgcggtg gcgtttccga
    17821 cggacatggt cggcgagacg gccgatcccc aggaggtcgt cgctggcgaa aagacgccgg
    17881 ccgtcgtcca gggaaagccg ggtcccggat tcgactttgt tcccgaggga tcggagg
//
LOCUS       DAOTFD010000030        17352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2218_length_17352_cov_4.722726, whole genome shotgun sequence.
ACCESSION   DAOTFD010000030 DAOTFD010000000
VERSION     DAOTFD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_17352_cov_4.722726"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="P5079_03395"
     CDS             <1..385
                     /locus_tag="P5079_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY29063.1"
                     /translation="ALHRPKRFFGAARNIEEGGSLTIMATALVETGSRMDDVIFEEFK
                     GTGNMEIVLSREISRLRIFPAVDIPKSGTRKEELLLDPEELEAVRKMRRALAQFTPVD
                     AARELAEQLKSFPSNKEFLAAVRKM"
     gene            471..1445
                     /locus_tag="P5079_03400"
     CDS             471..1445
                     /locus_tag="P5079_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRY29064.1"
                     /translation="MSEFSAMLKSFSSFTENLRSPQSKSMRRLKWLAGAATVLLLMTV
                     SVDTKSFYEFVPQWDAPLVAAVQSEINRFNGRVGFYLKDLQTGRTLAINADELFPSAS
                     LVKVPIMMGVFKKIAAGEISLDTTLVLRRDDKVGGSGILKRCRSGSRWTVERLLRLMI
                     SRSDNTAARMLVNLTGIPYLNSTFQSLDLQVTRVQEEPLFRLTPDVSVENYTTPREMA
                     RLLENLYHGGCITPESSQRMMDIMKKGKARHRLARYLPRGWEIADKTGLLRGACHHIG
                     IVFTPHGNFLICVLTADNTDSEYGRAKQFIARVGRKVFRHRRDEYRHS"
     gene            complement(1566..2441)
                     /locus_tag="P5079_03405"
     CDS             complement(1566..2441)
                     /locus_tag="P5079_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29065.1"
                     /translation="MRSFIAVGVILFLPGVLPPQKAEAGSFQNFSGKYHVIGSESVRS
                     GGRRAGALSSSHASFQYQWKTVLLRKDAVSLGFFYDRLDLASSPAILLPDRLHSLRAR
                     IGLEKQWGPRVALRFEVAPGLASDMEDVGWKDAVVGGFAGMSLVQNPDLIWLVGVSGS
                     SSAEYKIMGIAGVRWQISRRWLLSAMPPEVRLKFSPAPPVDFFIGPFFHSDVYRVSDR
                     FGAKRNIALLDGAHLQARDWGMEGGWVWRPRPAISLEGSVGYCFYRRYAFEDTPHRYE
                     GFSAPAAGLSLNARF"
     gene            complement(2574..3893)
                     /locus_tag="P5079_03410"
     CDS             complement(2574..3893)
                     /locus_tag="P5079_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29066.1"
                     /translation="MSKAVWGCALTGFLLCSAPFLPVASAGEVDEDALFSSPEMVTQP
                     AGSAVPEGGKKTIGFSGEMTGVTETTAIKASTQNIHHSYLVANGFIDARLKNDMKAFA
                     NLETVHDPEKSSNTFEVRELFFDFNVDRRVYLRTGKQVLQWGRCGLWNPTDLVNIERK
                     RFIRKIGYREGAYGLKLHAPFGARQNIYGFLDTGEAPTLEDLGGALKYEFLVGNTEMA
                     FSGWAKKTYDPVWGYDFSTRLGEVDVVGEASVSRSSNKRQIFINQGMLDVRSKEDEWT
                     PRASINFSRGFRMGNFNDRLRLSTEFFYDHSGYKKGFLTDSTAYPFAAPLVTRDAAGN
                     IVTRTVGTKASFLLDNDLYEMNQLSRHYGALFLTFGRFLLSDMALNVNYVRNFDDSTG
                     VLSSGVTYRNLSDLTLGLLVNTFTGPAGEYTYPMMKYNVQLTGGISF"
     gene            complement(3929..4693)
                     /locus_tag="P5079_03415"
     CDS             complement(3929..4693)
                     /locus_tag="P5079_03415"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY29067.1"
                     /translation="MRLFLAFLVVFGTSFPAFAAEGRIVPILQAIDESYKLKNDVTAQ
                     VMLTQQKPEQGTKVIEMLYYRRDSDDSFLIVMTAPENEKGNGYLRVGDNFWMYRKNTR
                     TFQHVNRDESIGGTDANSGDFETRKLTELYAPAVDAAGKEKISEEMLGQIPVHKSEVK
                     AKVNDVDYPKKIFWTRKDNNLLLKQESYSLSDTLMETAYFLKFTVIDGRYVAVKHLYI
                     DEFEKGNKTVVDISNIVSKELDDSIFTKAYLENLSK"
     gene            complement(4705..6468)
                     /locus_tag="P5079_03420"
     CDS             complement(4705..6468)
                     /locus_tag="P5079_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY29068.1"
                     /translation="MMLLVRLSLRNLFRQKRRNVLLGSAMALGIMILIVANSFSHGIS
                     DIIFNKIMPYVAGHVAVRFNEKGGVMAEIFRDKARALAAVANEPLIERTDESVGFFMR
                     AIGNGKADNIILVGADTTRGVSKKELKELEESFKMVDGTWKDLETTAVENAAILSTDK
                     ARYLNVKKGDVLRIRFRNMFGQDQAARLNVVGIVKNSNIFMQSVVFVEQRRAKDLLGY
                     RSYETASLNLRIKNPQKNSRIIADRIHSRLKPGLAYIQGTVSNKNKEQTVVVFGYKSD
                     DDSKEKFSKKLKITDGTLENALKKNGVVLCRPLARALGAAAGDEISLRYDRKFEPEPA
                     RFACKVSAVCEPTEGLGENVVVMNDDKFYNVYYESLPKNDDATPPAYLPKEGDAWYAL
                     LSPEWILLKRTANTDEYTKKYKEMGKKKRKSTLIDVSTMYETASDILKLEGVLNLITL
                     SAVLVLFFIILLGVVNTLRMTIRERTREIGTIRAIGMQKNDVRNMFVLETFFLTVFSA
                     AAGVVGGFAVMGLLKQMTFNMSDNPFGMLLVDSHLHFVPTVLGVLGNVLLIVLVAVLT
                     AYFPARRAANLSPANALRHYE"
     gene            complement(6472..8031)
                     /locus_tag="P5079_03425"
     CDS             complement(6472..8031)
                     /locus_tag="P5079_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY29069.1"
                     /translation="MKLVLTIAWRNILRHRGKSLVIGVILFLGALLMTVGNGVISGMA
                     AGIEKNIVNGFMGDIVLISEKQKSENILLDFMGQAIEPITTYKKIKPVLQNQDIVARH
                     LPVGKNLTLVLKEDEATPGGAFLLGVDFAAYQKMFPDNFRIIEGRWPRPGERGLLVPT
                     FAREEMANYMNLWVIPENGTVIKEHMKKETLEHIDALTVSSNVVMMGMASGMNSTSDI
                     RFPVTGIVKYNALSTIFGHFCIADIDSYRECLGYFTAADKAVAVPKEEQKLLNLEGTD
                     LDSLFGVDPLIVSNTRAPGGAAVPGGAAIINNAIVSGSSVTATPLGVENGIYNLVLVK
                     LKDGVPYKTALGRLNRALAEAQTGVRAITWSKASGPIGSMALIIRGALFVFVTLLFCV
                     AVIIIVNTLTMAALERTSELGMMRAIGARKSFIGGMFLGETAILSSIFGGAGIVAGIA
                     AVKLIPLFKITSTNDMVQILYGGDIFHPLLGTSDVLITLMQLVFVTIIAAVYPMHVAG
                     SITPLDAITRD"
     gene            complement(8153..8830)
                     /locus_tag="P5079_03430"
     CDS             complement(8153..8830)
                     /locus_tag="P5079_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29070.1"
                     /translation="MNIIEIKGLKKDYPLGNTVVKALRGIDIAVEEGEFMSIVGPSGS
                     GKTTLLNVIGCIDFATEGSVKVGDREITVLNDRQITDIRLNKIGFIFQTFNLIPVLNV
                     IENVEFPLLLMKNQSRTEIRRRADRLIGEVGLTEFAEHRPAELSGGQRQRVAIARALV
                     TNPDIVLADEPTANLDSVTGAAILELMKEMNRIEKTTFVFSTHDANVLKYARRIVKIK
                     DGLLSDN"
     gene            complement(8827..9384)
                     /locus_tag="P5079_03435"
     CDS             complement(8827..9384)
                     /locus_tag="P5079_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY29071.1"
                     /translation="MPSANKTQYAILGMLSFGPRSGYDIKKIIDRSLRFFWSENYGHI
                     YPILKRLEKRRWVRRQAAAPTGRRKRNVFALAPAGRAELRAWLAKPAADLPVRNEMVL
                     KLFFGRQMGRRTALSLLAAEEKRNAERLAQLERIEKEISRREAPDLPYWLATLRLGIA
                     VTRTFRGWCRDTAGRFGSLKSGRRA"
     gene            9540..10283
                     /locus_tag="P5079_03440"
     CDS             9540..10283
                     /locus_tag="P5079_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29072.1"
                     /translation="MSHVLSVTQLRKNYGDTVAVDGVSFQVGRNEIVGLLGPNGAGKT
                     TTINMILGVLEPTAGDIRIEGLDAATHRARAMERTNFAAVYAPLPGNLTVFQNLRIFG
                     LLYGVPNLAERIETLIAHFDLEPFRRVKCGLLSSGEQTRVGLAKALLNRPVLLLLDEP
                     TASLDPATARDVRRKIRSFAADGSGGVLWTSHNMYEVEEVCDRVLFLSHGKILLEGDP
                     KTLPRDHGKKTLEDLFVAVAREPLTLGAA"
     gene            10280..11089
                     /locus_tag="P5079_03445"
     CDS             10280..11089
                     /locus_tag="P5079_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29073.1"
                     /translation="MNGAVQRTAAIALRQYYLMRGSFSRVLPIFAWVAIDMVLWGYMT
                     RYLNAITAAGFDFVPALLGAVLLWDFFIRVMQGVTMAFFEDVWSRNFLNLFTTPLTIT
                     EYVGGLVFTSIATSVVGLAAMLSLASTVFGLSMLSYGTMTLPFLLVLFLFGIALGVAG
                     VALVLRFGPAAEWFIWPIPAMISPFAGVFYPISTLPGWMQTVAQGLPLAAVFENLRAM
                     VAGKEPSMPALLWSGCLAGLYVLAAVWSFRHVYRHAVRTGVIARYSAETAS"
     gene            complement(11149..12126)
                     /locus_tag="P5079_03450"
     CDS             complement(11149..12126)
                     /locus_tag="P5079_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29074.1"
                     /translation="MTTSFLQFIEHLFGDQLVTVSRTLHLMALIVLAACWSLPVVFHY
                     LRLAQVSWGALWVPMWLCRKCGHYSPPDYVECRQCKAPMTTAWWEKYLPGFFADSARH
                     GGRFLLFWYRIAGLVVFYVGTLLAFWTLRFYSFAQYPLQELMAVLAVVTLLLTMGFGG
                     RALGAQKGSIVARGMDLVAGAGMAALTGLWLFLWAAAPFPPGKPLAYVQALTGGRVHL
                     MTPGGLQASADGNLADGGTRFSIQYAVVRWPLFHIHQPFVTRLVSEPVLDPWTLLTFD
                     AAASVFRREDPYRPRVLLQSRSFRVEPGRTYSLREPSEGADWLLEETSK"
     gene            complement(12175..12987)
                     /locus_tag="P5079_03455"
     CDS             complement(12175..12987)
                     /locus_tag="P5079_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29075.1"
                     /translation="MKIEETDRRLVLREDRLFALIVFAVLAAVFFVFTVGVMVFDLSR
                     CDVSLALPAAALVWLGSVVLLCAADYRIVLVFDKDARTLVWTRKALFRKREDAFRFDE
                     FQIFSSEPAFWENTRTVTVRTGEGAVPLISCARERADEAAERMSEFLESAVRGGPTSR
                     GPGGVSSGAGAPVHDAVPGLCKVFTAVVAVAVLIIFYGGISPRFEGRPPSHGVLAGLL
                     MAGVVYFAFAAAWLVAVRRSEDQRRPSRHFAFSILFLMIVLALMSVCGVVKP"
     gene            13576..16731
                     /locus_tag="P5079_03460"
     CDS             13576..16731
                     /locus_tag="P5079_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY29076.1"
                     /translation="MRQDRAVSFFVHLSSLKPNGFFPPLPGSLRKLFFILTAAGLWSL
                     LVPSLRADTAALSVLDGWYAASSATLSSLGHVTRLNTEDNGLDVALAPGKLLSAHKWI
                     GVRFSAFTLPDGYLYAGVTVKFRMRRTGTPTEVYCDVSASSTTTKWSDAFQDNETGEN
                     INITTHTLGSANFAEFSHNAGGVVTSTHQVNSMEVLLRNADASPSVWVDTVNVEVTYT
                     KAATVLTAETVDDDLDGKIDAYHVTFSTKMMDSSLAATAPGFAVAGYTNLTFHSTGTP
                     AHADTADDADIYLRFAEGTTGDTGQTPDLTYSSSTGAMFTLSGYRLLNVASGDVTETD
                     KAAPQMLSATASDASGGGGGIQAGDVVQIIFSENTTSPLLTSANINAVLSLSSAHTWL
                     DASNALGSAVWEQPNILTVTLSAAGGPPTVAVGDTLTLDGATVKDAANNVSAGSRAVG
                     GDFGSDSTPPVIASRETADLDSDGWIDAIHVTFNEKILDSSVTAADFDVADVTGESFA
                     STTHGDTIDDADIYITFTDGLLGTDATPTVSFASGTFCDLSNNKMASSPATPSSDRAP
                     PALLSAAADDSAYLATGIDGDDTVTLVFSEKTNAPTVDASNIDAVLPLSGGHTWKDGN
                     GTVGGAAWNSPAKNTLTVQLSGGAGLPTVAVGDTLSIPAGLIEDVLGNKTTDAALIGG
                     TFTGTDTTPPQILSVYPEDKSANTSVTATPRVRFSENMNADSARTAFTLQITRDRDGK
                     NRNAAVSGATVFSSADNTLTFTPAEPLESGSAYQITVSTSAQDTIGNPLGTSHTSRFR
                     TLLDSASSNLVVAEDEKSRVYLPSGATTVSVAVRIGLDPVGQPQRVDPQKIEEANRKI
                     RSDAYRANDPLTGALREICLYDSQGAPLDLRFAVPATVSIPYTETGGFVDGTSPLLPE
                     DRLSMYCLDETNALWVRIPDSSVNTADNLVTAPTPHFSVFGLFGNPHSGGNSDLYAFP
                     VPFRRGRDSVVTFVGLPIDATVKIHTLSGTRVRTLPRAGGAQETWDLKNDDGDTVADG
                     VYLYVVENGGFRATGKLLVTR"
     gene            16866..>17352
                     /locus_tag="P5079_03465"
     CDS             16866..>17352
                     /locus_tag="P5079_03465"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29077.1"
                     /translation="MLTLGAGSRALGMAEASAGLADDAFGLFGNAAGLGFLRDSEVSA
                     AYHPLGEDIANRSVVYAHPLSSRPGAWAVDGSYLDVTDIRGYSPGGEKTGDVKASDLV
                     FGAAYGRPVANHLAAGLRFKGLRETLDGRTAATFAADAGLLYRPPRGAGAGLSVQNVG
                     PG"
BASE COUNT         3419 a         5494 c         5164 g         3275 t
ORIGIN      
        1 cgcgctccac aggcccaagc gctttttcgg cgcggcccgc aacatcgagg agggaggcag
       61 cctcaccatc atggctaccg ccctggtgga gaccggcagc cgcatggacg acgtgatctt
      121 cgaagagttc aaaggcacgg ggaacatgga gatcgtcctt tcgcgcgaga tatcgcgtct
      181 acggatattc ccggccgtgg acatccccaa gagcggcact cgcaaagagg aactcctgct
      241 cgaccccgaa gaactggagg cggtccgaaa gatgcggcgg gcgctggccc agttcacgcc
      301 cgtggacgcc gcccgggaac tggcggaaca attgaagagt ttcccctcca acaaggaatt
      361 ccttgccgcc gtccgcaaga tgtaaccccc cgccgccggc gatgaggccc ccgatccggg
      421 tcgtcccggc cgccgattag accgttttcc ttgatattcg gacgataaat ttgtcagaat
      481 tttccgccat gctgaaatcc ttctcctcgt tcaccgaaaa cctgcgttcc ccgcagagca
      541 aatcgatgcg ccgtttgaaa tggctggccg gcgcggcgac ggtgcttctg ctgatgacgg
      601 tgagcgtgga caccaaatcc ttctacgagt tcgtgcccca atgggacgcg ccgctggtgg
      661 cggccgttca gtccgaaata aacagattca acggccgcgt ggggttctac ctgaaagacc
      721 ttcagacggg ccgcacgctc gccatcaacg cggacgaact cttccccagc gccagcctgg
      781 tgaaagtgcc catcatgatg ggcgtcttca aaaagatcgc cgccggcgag atttcgctcg
      841 acaccacgct ggtgctccgc cgggacgaca aggtgggcgg gtccggcata ctgaagcggt
      901 gccgctccgg aagccgatgg accgtggaac ggctcctgcg gttgatgatc agccgcagcg
      961 acaacaccgc cgcccgcatg ctggtgaacc tgacgggcat cccttatctg aacagcacgt
     1021 tccagtcgct cgacctgcag gtcacgcgcg tgcaggagga accgctcttc cgcctgacgc
     1081 cggacgtttc ggtggagaac tacaccacgc cgcgggagat ggcccgcctg ctggaaaacc
     1141 tctaccacgg cggctgcatc acgccggaat cctcccagcg gatgatggac atcatgaaaa
     1201 aaggcaaggc gcggcaccgc ttggcgcggt atctgccgcg cggctgggag atcgccgata
     1261 agaccggcct cctgcgcgga gcctgccacc acatcggcat tgtctttacc ccccacggca
     1321 atttcctcat ctgcgtgttg accgcggaca acaccgactc cgaatacggc cgggccaaac
     1381 agttcatcgc ccgcgtcggg cggaaagtct tccgccaccg ccgcgacgaa taccgtcact
     1441 cctagaccct ccccgtccga atcccgaaaa tacgttgagc ccctctccac ggtgacaagc
     1501 gagagggata tccgttcggg gttggcggct gttccgacgg cgggcggggg tgcggaggac
     1561 ggggtctaaa agcgggcgtt cagcgatagg ccggcggcgg gggccgaaaa accctcgtac
     1621 cggtggggcg tatcttcaaa agcgtagcgg cggtaaaaac aatatcccac ggaaccttcc
     1681 agtgaaatgg ccggccgcgg gcgccacacc caaccgccct ccatccccca atcccgcgcc
     1741 tgcaaatggg cgccgtccag cagagcgatg ttccgtttgg ctccgaacct gtccgaaacg
     1801 cgataaacgt cggaatggaa gaacggtccg ataaaaaaat cgaccggagg cgcgggactg
     1861 aacttaaggc ggacttccgg cggcatggcg gagaggagcc atcgcctgct gatctgccag
     1921 cggacccccg caatgcccat gatcttgtat tccgcggagg aactcccgga aacgccgacc
     1981 agccagatca ggtccgggtt ctgcaccaac gacatgcccg caaatccgcc caccacggcg
     2041 tccttccagc cgacgtcctc catgtcgctg gccaatccgg gcgcgacctc gaaacgaaga
     2101 gccacgcgcg gcccccattg tttttcgaga ccgatgcgcg cccggaggct gtggagacgg
     2161 tccggcaaaa gaatcgccgg cgaagacgcg agatcgagcc ggtcgtagaa aaaccccagg
     2221 gaaacggcgt ccttccggag caagaccgtt ttccattgat actgaaaaga cgcgtgactc
     2281 gacgacaagg cccccgcgcg gcggccgccg gaacggacgc tttcggaacc gatgacgtgg
     2341 tattttccgg aaaagttctg aaaggacccc gcctctgctt tttgcggcgg gaggaccccc
     2401 ggcaggaaga ggatgacgcc cacggcgata aagctcctca cggccgccga aacccgcccg
     2461 gttttaaaga cccgccgttt ccgggtccga cgcatctttt tttgaaggaa tcgaaaccac
     2521 cccgccgcga tcatcgaggt ggccgatgag agcggcatcc gcaaccgatt cggtcaaaaa
     2581 gagatcccgc cggtgagttg aacgttgtac ttcatcatgg gataggtgta ttctccggcc
     2641 ggaccggtga aggtgttgac gagcaagccc aacgtcaggt cggaaaggtt gcggtacgtc
     2701 acgccgctgg agagcacgcc cgtgctgtcg tcgaaattac ggacgtaatt gacgttgagg
     2761 gccatgtcgg acaggaggaa acggccgaag gtgaggaaaa gggccccgta gtgccgggag
     2821 agctggttca tctcgtagag gtcgttgtcg agaaggaagg aggccttggt gccgacggtt
     2881 cgggtgacga tgttgcccgc ggcgtcccgc gtgacgagag gcgcggcgaa ggggtaggcc
     2941 gtcgaatcgg ttaaaaatcc ctttttatag ccggagtggt cgtagaagaa ttcggtcgac
     3001 agccggaggc ggtcgttgaa gttccccatc cgaaaacccc tggaaaagtt gatgctggcg
     3061 cgcggcgtcc attcgtcctc tttggagcgg acatccaaca tcccttggtt tataaaaatc
     3121 tgccttttat tgctgcttcg ggaaacgctg gcctccccca ccacgtccac ctcgccgagg
     3181 cgcgtggaaa aatcgtatcc ccagacgggg tcgtaggttt tcttggccca accggaaaaa
     3241 gccatttccg tgttccccac cagaaattcg tatttgagcg cgccgcccag gtcctccaac
     3301 gtgggggcct cgccggtatc caaaaaacca taaatgttct gccgggcgcc aaacggcgcg
     3361 tgaagtttca agccgtacgc cccctcgcga taaccgatct ttcggatgaa acgtttcctc
     3421 tcgatgttga cgaggtcggt ggggttccac aggccgcatc ggccccattg caggacctgc
     3481 ttgcccgttc gaagataaac gcgccggtcg acgttgaaat cgaagaaaag ctcgcggacc
     3541 tcgaacgtgt tgctgctctt ttcggggtca tggacggttt ccaggttggc gaaggccttc
     3601 atgtcgttct tcaggcgcgc gtcgatgaac ccgttggcca cgaggtagga atggtggatg
     3661 ttttgcgtcg aagccttgat ggcggtcgtt tccgtgacgc ccgtcatttc tccggaaaac
     3721 ccgatggtct tctttccccc ctccggaacc gcggagcccg ccggctgagt gaccatctcc
     3781 gggctggaaa acagagcgtc ctcgtcgact tcgccggcgg acgcgacggg aaggaacggg
     3841 gccgagcaca aaaggaaacc ggtcaacgcg cacccccaaa cggccttcga catgacgtgt
     3901 tttccttcgt gaaccgaacg gacgtccgtc atttgctcag gttctccaga tacgctttgg
     3961 tgaaaatgct gtcgtccagc tctttggaaa cgatgttgga aatgtccacc acggttttgt
     4021 tgcctttttc aaattcgtcg atatagaggt gtttcacggc cacataacgg ccgtcgatga
     4081 cggtgaactt caaaaagtag gccgtctcca tgagcgtgtc ggaaagcgag taggattcct
     4141 gtttcaggag gaggttgttg tcttttcggg tccagaatat tttcttggga taatccacgt
     4201 cgttcacttt ggccttgact tccgatttat gcacggggat ctgtcccaac atttcctccg
     4261 atatcttttc ctttcccgcc gcgtcaaccg ccggggcata gagttcggtc aatttgcgcg
     4321 tttcaaaatc cccgctgttg gcgtcggtgc cgccgatgct ttcgtcgcgg ttgacgtgct
     4381 ggaaggtgcg cgtgttcttc cggtacatcc aaaagttgtc ccccacgcgc aggtagccgt
     4441 tccccttttc gttctccggg gcggtcatca cgatgaggaa ggaatcgtcc gaatccctcc
     4501 ggtaatacag catctcgatc accttggtgc cctgttccgg cttctgctga gtgagcatca
     4561 cctgggcggt cacgtcgttc ttcaacttgt aactttcatc gatggcctgc aggatgggga
     4621 cgatgcggcc ctccgcggcg aacgcgggga aggatgtccc aaaaacgaca agaaacgcga
     4681 ggaaaagtct caaccgatcc tccctcattc gtagtggcgc agggcgttcg ccggggacag
     4741 gttggccgcc cgccgcgccg ggaaataggc ggtgagcacc gcgacgagca cgatgagaag
     4801 cacgttcccc agaacgccca agaccgtggg cacaaaatgc aggtggctgt ccaccaacaa
     4861 catgccgaaa ggattgtccg acatattgaa cgtcatctgt ttcaggagtc ccatcaccgc
     4921 aaacccgccg acgaccccgg cggcggcgga aaaaacggtc aaaaagaacg tctccaggac
     4981 gaacatgtta cggacgtcat ttttctgcat gccgatggcg cggatggtgc cgatctcccg
     5041 cgtacgttcg cggatggtca tgcgcagcgt gttcaccacg cccaaaagga tgatgaagaa
     5101 caacaccaac accgccgaca aggtaatgag gttcaaaacc ccttccaatt tcaagatgtc
     5161 gctggctgtt tcatacatgg tggagacgtc gatgagcgtg gatttccttt tcttcttgcc
     5221 catttcctta tatttttttg tgtattcgtc cgtgttggcg gtccgtttga gcaatatcca
     5281 ttccggagag aggagcgcat accaggcgtc cccctctttt ggcaagtagg ccgggggggt
     5341 ggcgtcgtcg ttcttgggca ggctttcgta atacacgttg taaaatttgt cgtcgttcat
     5401 cacgacgacg ttttccccca acccctccgt cggttcgcaa acggcgctga ccttgcaggc
     5461 gaaccgggcc ggttccggtt caaactttcg gtcgtagcgc agggagattt cgtccccggc
     5521 cgccgccccc agggcccggg ccaggggccg gcaaaggacc acgccgttct ttttgagggc
     5581 gttttccagc gtaccgtcgg taattttcaa cttttttgaa aatttttcct tcgaatcgtc
     5641 gtcgctttta tatccgaaaa caacgacggt ttgctccttg ttcttattgg aaaccgtccc
     5701 ctggatgtag gccaggccgg gtttgaggcg gctgtggatg cggtccgcga tgatgcggga
     5761 atttttttgc gggttcttga tgcgcaggtt cagcgaagcg gtttcgtagg aacgataccc
     5821 cagcaggtct ttggcccggc gctgttcgac gaacacgacc gactgcatga aaatgttgct
     5881 gttcttgacg atcccgacaa cgttgagccg cgcggcctgg tcctggccga acatgttgcg
     5941 gaaccgtatc cggaggacgt ctcccttttt gacgttcaaa tagcgcgcct tgtccgtcga
     6001 caggatggcc gcgttttcaa cagcggttgt ttccaggtct ttccacgtgc catcgaccat
     6061 cttgaaagac tcttcgagct ctttgagttc ctttttgctg accccgcggg tcgtgtcggc
     6121 ccccaccagg atgatgttgt ccgctttgcc gttcccgatg gcccgcatga agaaaccgac
     6181 ggattcgtcg gtccgttcga tgagaggctc gttggccacc gcggccagcg cgcgggcctt
     6241 gtcgcggaag atctccgcca tcacgccgcc tttttcattg aaacggacgg ccacatggcc
     6301 ggccacgtac ggcatgatct tgttgaagat gatgtcggaa atgccgtgcg agaacgagtt
     6361 ggccacgatc aggatcatga tgcccagggc catggcgctg cccaggagaa cgttgcgccg
     6421 tttttggcgg aagaggttgc gcaggctgag tcggacaaga agcatcatga atcagtccct
     6481 cgtgatggcg tccagcggag tgatgctgcc cgccacgtgc atgggataga ccgcggcgat
     6541 gatcgtcacg aagaccaatt gcatcagggt gatcaggacg tccgaagtcc ccagcaacgg
     6601 gtgaaagata tcgccgccgt agagtatctg caccatgtcg ttggtggaag tgatcttgaa
     6661 gagcggtatc aatttcaccg ccgcgatccc cgcaacgatg ccggcgccgc cgaagatcga
     6721 cgataaaatg gccgtttcgc cgaggaacat gcctccgatg aacgatttgc gcgcgccgat
     6781 ggcgcgcatc atgcccagtt cggatgtgcg ttccagagcc gccatggtga gcgtgttcac
     6841 gatgatgatg acggccacac agaataaaag cgtgacaaaa acgaacaggg cgccccggat
     6901 gatgagggcc atgctgccga tggggcccga ggccttgctc caggtgatgg cgcgcacgcc
     6961 ggtctgggcc tcggcgagag ctcggttgag ccggcccaag gcggttttat aggggacgcc
     7021 gtccttcaat ttaacgagaa caagattgta aataccattt tcaacaccca gcggtgtggc
     7081 ggtgaccgaa ctgccagaaa caattgcgtt gttaatgatg gccgccccgc cggggaccgc
     7141 cgccccgccg ggcgcacgcg tgttggacac gatgagcgga tcgacgccga acaaggaatc
     7201 cagatcggtc ccttccaggt tgaggagttt ctgttcttct ttcggcaccg ccacggcttt
     7261 gtcggcggcc gtgaaatagc ccagacactc acgataggaa tcgatgtcgg cgatgcagaa
     7321 atggccgaag atcgtgctga gcgcgttgta tttgacgatc ccggtgacgg ggaaacggat
     7381 atcgctggtg gagttcatgc cgctcgccat gcccatcatg acgacgttgg aactgaccgt
     7441 cagggcatcg atatgttcca gcgtttcttt tttcatgtgt tccttgatca ccgtgccgtt
     7501 ttcggggatg acccacaggt tcatataatt ggccatctct tcccgcgcaa aggtgggcac
     7561 cagaagcccc cgttccccgg gccggggcca gcggccttcg atgatgcgga aattgtcggg
     7621 gaacatcttt tggtacgcgg cgaagtccac gcccagaaga aaggcgccgc ccggcgtggc
     7681 ctcgtcttct ttaaggacca gggtcaggtt cttgcccacg gggaggtgcc gggccacgat
     7741 gtcctggttt tgcagaacgg gcttgatttt tttgtaagtg gtgatgggct cgatggcctg
     7801 gcccatgaaa tccagaagga tgttctcgga tttctgtttc tcgctgatca ggacgatgtc
     7861 gcccatgaac ccgttgacga tgtttttctc gatgcccgcc gccatgccgg agatcacgcc
     7921 gttgccgacg gtcatcaaca gcgcccccag gaacaggatg acaccgatga cgaggctctt
     7981 gccccggtgg cgcaggatgt tgcgccaggc gatggtgagg acgagtttca taaattacct
     8041 tttgtcattg cgaggagcat gtttaacacg cgacgaagca atcgggtttg aagccggttc
     8101 gccgcgccct tttaggactc gcgatgacag gccgaggaat atttccgtca tgtcaattat
     8161 cggagagcag tccgtctttg atcttgacga tgcgccgggc gtatttcagg acgttggcgt
     8221 cgtgcgtgga aaagacgaac gtggttttct cgattcggtt catttccttc atcaactcca
     8281 aaatggccgc ccccgtgacc gaatccaggt tggcggtggg ttcgtcggcc aggacgatgt
     8341 cggggttggt gacgagcgcc cgggcgatgg ccacgcgctg gcgttgcccg ccggaaagtt
     8401 ccgccggtcg gtgttcggcg aattccgtga ggcccacttc cccgatgagg cggtccgccc
     8461 gccgccggat ctccgtcctg gactgattct tcatcaacaa cagcgggaat tccacgttct
     8521 cgatcacgtt gaggacggga ataaggttga aggtttgaaa gatgaaaccg atcttattga
     8581 gccggatgtc cgtgatctgc cggtcgttca gcacggtgat ctcccggtcc cccaccttga
     8641 cgctgccttc cgtggcgaaa tcgatgcagc cgatgacgtt gagcagagtg gtcttgccgg
     8701 acccggacgg gcccacgatg ctcatgaact ccccctcctc cacggcgatg tcgatccccc
     8761 gcaaagcttt caccaccgtg ttgcccagcg gataatcctt tttcagcccc ttgatttcaa
     8821 tgatgttcat gcgcgcctcc cggacttcag tgatccaaat cttccagcgg tatcgcggca
     8881 ccacccccgg aacgtccttg tcacggcgat accgagccgc aaggtggcca gccaataggg
     8941 caaatccggc gcctcccgcc gggagatctc tttttcaatc ctctccagct gggccagcct
     9001 ttcggcgttc cgtttttcct cggccgccag gagagacagc gcggtccgcc ggcccatttg
     9061 ccggccgaaa aaaagtttaa gcaccatctc gttgcgcacg ggcaggtcgg ccgccggttt
     9121 ggcgagccag gcgcgcaatt cggcccggcc ggccggggcc agcgcgaaca cgttgcgttt
     9181 gcgccggccc gtcggcgccg cggcctgccg gcgcacccat cgcctcttct ccagccgttt
     9241 caggatgggg taaatgtggc cgtagttttc gctccaaaaa aaccgcaggc tgcggtcgat
     9301 gatttttttg atgtcgtatc ccgaccgggg cccgaaactg agcattccca aaatggcgta
     9361 ttgcgtcttg ttggcggacg gcatggctgt gtcgccaaat ataacacaat gatatatagc
     9421 gcgtcaagcc cgccggggcc ctcgccggac acggtgaaat gtaatatcat gcttccggct
     9481 tcgccggtcc gccggcggac ctttcaaaag acgaacatcc ggcaggagcg tcccctctca
     9541 tgtcccacgt gctttccgtc acccagttgc gcaagaacta cggcgacacc gtcgcggtgg
     9601 acggcgtctc gttccaggtg ggccgcaacg agatcgtcgg cctcctgggg ccgaacgggg
     9661 cgggaaagac caccaccatc aacatgatcc tcggcgtcct ggaaccgacc gccggagaca
     9721 tccggatcga agggctggac gcggcgacgc accgggcccg ggcgatggaa cgcacgaatt
     9781 tcgcggcggt ctacgccccg ctgcccggca acctcaccgt gttccagaac ctgcgcatct
     9841 tcggcctgct ctacggggtg ccgaacctcg ccgaacgcat cgaaaccctc atcgcccact
     9901 tcgacctcga accgttccgg cgggtgaagt gcggcctgct ctcttcgggc gaacagacgc
     9961 gcgtggggct ggccaaggcc ctgctcaacc ggccggtcct gcttctgctg gacgaaccca
    10021 ccgcgtcgct ggaccccgcc acggcgaggg atgtccgccg caagatacgc tccttcgccg
    10081 ccgacggctc gggcggcgtg ctgtggacct cgcacaacat gtacgaggtg gaggaggtct
    10141 gcgaccgggt gcttttcctc tcgcacggga aaatcctgct ggagggcgac cccaagaccc
    10201 tgccgcgcga ccacggcaag aaaaccctgg aagacctctt cgtggccgtg gcgcgcgagc
    10261 cgctgacgct gggggccgca tgaacggcgc cgtccaacgc accgcggcca tcgcgctgcg
    10321 ccagtattac ctgatgcgcg ggagtttctc ccgggtactg ccgatcttcg cctgggtggc
    10381 catcgacatg gtgctgtggg gatacatgac ccggtatctc aacgccatca ccgccgccgg
    10441 gttcgatttc gtgccggcgt tgctgggagc ggtgttgttg tgggatttct tcatccgcgt
    10501 gatgcagggg gtgacgatgg cgttcttcga agacgtgtgg tcgcgcaact tcctgaacct
    10561 gttcaccacg ccgctcacca tcacggagta cgtcggcgga ctggtgttca cgagcatcgc
    10621 caccagcgtg gtgggcctgg cggcgatgct ttcgctggcc tcgacggtgt tcgggctgtc
    10681 gatgctctcc tatgggacga tgacccttcc gttcctgctg gtgctgttcc tgttcggcat
    10741 cgcgctgggc gtggcggggg tggcgctggt gctgcggttc ggtccggcgg cggaatggtt
    10801 catctggccc atccccgcca tgatctcccc cttcgccggg gtcttctacc ccatttcgac
    10861 gctgccgggc tggatgcaga ccgtcgcgca ggggctgcct ttggccgcgg tgttcgagaa
    10921 cctgcgggcc atggtggccg ggaaggaacc gtcgatgccg gccctgttgt ggagcggatg
    10981 tctggcggga ctctacgtgt tggcggcggt gtggtctttc cggcacgtgt accggcacgc
    11041 ggtgcgcacg ggcgtcatcg cccggtacag cgccgaaacc gccagttaag gcgccgcccg
    11101 cggcggaaac cgcgcgggcc ggtcaattct taaaacgggc ggacgcggtt atttggaggt
    11161 ttcttcgagg agccagtcgg ccccctcgga gggttcccgg agggagtagg tccggcccgg
    11221 ctcgacgcgg aaagagcggg actggaggag gacgcgcgga cgatagggat cctcgcggcg
    11281 gaaaacgctc gcggcggcgt cgaaggtcag cagggtccag gggtccagga cgggttccga
    11341 caccagccgc gtgacgaacg gctgatggat atggaacagg ggccaacgga cgaccgcgta
    11401 ttggatggaa aaacgggtcc cgccgtccgc gaggttgccg tcggcggacg cctgcagtcc
    11461 tccgggcgtc atgaggtgca cccggccgcc ggtcagcgcc tgcacatagg cgagcggctt
    11521 gccgggaggg aacggcgccg ccgcccacag gaacagccac agcccggtga gcgccgccat
    11581 ccccgcgccc gcgacaagat ccattccccg cgccacgatc gaacctttct gcgcgcccaa
    11641 cgcccttccc ccaaaaccca tcgtcagcaa caaagtcacc acggccagca ccgccatcag
    11701 ttcctgcagg ggatactgcg caaagctata aaaacgcagc gtccaaaaag ccagcagggt
    11761 gccgacatag aacaccacca acccagcgat ccggtaccaa aaaagaagga agcggccgcc
    11821 gtgccgggcc gaatccgcaa aaaaacccgg caggtatttt tcccaccacg ccgtcgtcat
    11881 cggggccttg cactgccggc attccacgta gtccggcgga ctgtaatggc cgcatttccg
    11941 gcacaaccac atgggcaccc agagcgcccc ccacgagacc tgcgccaagc gcaggtaatg
    12001 gaacaccacc ggcaggctcc agcacgccgc taggacgatg agcgccatca gatgcagcgt
    12061 gcgggacacc gtcaccagct ggtcgccgaa aagatgttcg atgaattgaa ggaaagacgt
    12121 cgtcatgtcg gaaaattata gcacacggga aaccttacgg acgggaaacc gcggtcatgg
    12181 tttcaccacg ccgcaaacgg acatcagggc caggacgatc ataagaaaga ggatgctgaa
    12241 ggcgaaatgc cgggacggcc tcctctgatc ctccgaacgc cgcaccgcca ccagccacgc
    12301 ggcggcgaac gcgaaataca cgacccccgc catcagcaac cccgccagga caccgtgaga
    12361 cgggggacgc ccctcgaacc gtggagaaat ccctccgtaa aaaatgatca acaccgccac
    12421 ggccacgacg gccgtgaaaa ccttgcataa cccggggacg gcgtcgtgca cgggcgcgcc
    12481 cgcgccgctg gaaacgccgc cgggtccgcg ggatgtcggc ccgccccgga cagcggattc
    12541 cagaaattcg ctcatcctct ccgccgcctc gtccgcccgt tcccgggcgc acgagatcaa
    12601 cggaacggcc ccctcgccgg tccgaaccgt caccgtccgg gtgttctccc agaaagcggg
    12661 ctcggagctg aaaatctgaa actcgtcgaa acggaaggcg tcttcccttt ttcggaagag
    12721 cgctttccgg gtccacacga gggtccgggc gtccttgtcg aacacaagga cgatccggta
    12781 atccgccgcg cacaaaagga ccacgctccc cagccacacc aacgccgccg ccggcaaagc
    12841 cagggaaacg tcgcaacggg agagatcaaa gaccatgacc ccgacggtga aaacaaaaaa
    12901 aaccgcggcc aacaccgcaa acacaatgag ggcgaaaagt cggtcctcgc ggaggaccaa
    12961 ccgccgatcc gtttcctcga ttttcattcc gttcctcccg gccggaaatc gccgatctca
    13021 agagggacga ccgaaccaaa caccgccgga ccccgtcttc tcccgagaga caccccttat
    13081 tataggtagg cgacaacctc ttggaaacat gtaaatcggt ttcagcttcc cttcggttct
    13141 cgtcccaacc gctgcttgaa ggggggcgat cccgcgttaa ttctttccag acaaggcggg
    13201 gatcgacttc gctccgccgc tcgactggtt cggggcgggc tcgctcgaag aatacgggta
    13261 ttcttcgagc gaccaccctg ccgccaggta tgatccctgt ttgtaggaag ggaaaagggg
    13321 ggcgagaaga tacgcgccac caaggaataa tcgccggtag attatagtta ttgtgactat
    13381 ttgttgtaac ttgtgacatt gacatccttg aattgcgggg atatacttgc tttagtcttc
    13441 ggtaacgtat catcgtccca tcaggggctt cccggacaaa accaagctgt ctcacacgac
    13501 acgtcgttcg attcggcggg tatttttcgg aatcctactc ataaaaacgg cgcgtatttt
    13561 ctgggggaaa tttaaatgag acaggaccga gcagtctcat tttttgtcca tctctcatcc
    13621 ctgaaaccca acggattttt tccgcccctc cccggttccc ttcggaaact ctttttcatc
    13681 ctgaccgcgg ccggcctgtg gtcccttctc gtcccctctc tgcgcgcgga caccgccgcc
    13741 ctctccgtgc tcgacggctg gtatgccgcc agcagcgcca ctctctcctc cctgggccac
    13801 gtgactcggt tgaacacgga agacaacggc ctcgacgtcg ccctcgcccc cggaaaactc
    13861 ctgagcgccc acaaatggat cggcgttcgt ttcagcgcct tcactctgcc ggacgggtac
    13921 ctttatgcgg gagtcaccgt caagttccgg atgcggcgga ccggaacgcc caccgaagtc
    13981 tattgcgacg tcagtgcctc ctccaccacc accaaatggt ccgacgcgtt tcaggacaat
    14041 gaaacaggcg aaaacatcaa catcaccacg cacactttag gcagcgccaa cttcgcggaa
    14101 ttcagtcaca acgccggcgg cgtcgtgacc agcacccacc aggtcaattc catggaggtt
    14161 ttgctgcgca acgccgatgc ctccccctcc gtgtgggtgg acaccgtgaa cgtggaggtc
    14221 acctacacca aggcggcgac cgttctgacc gccgaaaccg tggacgacga cctggacgga
    14281 aaaatagacg cgtaccacgt cactttttcc acaaaaatga tggattcctc gctggccgct
    14341 accgctcccg ggttcgcggt ggccggttac accaacctca cgttccacag caccggcacc
    14401 cccgcccacg cggacaccgc cgacgacgcc gacatctacc tgcggttcgc cgaaggaacg
    14461 accggggaca ccggccagac gccggacctc acctacagtt cctccacggg cgcgatgttc
    14521 acgctttcgg gataccggct cctgaacgtg gcctccggcg acgtgaccga aaccgacaag
    14581 gcggcgccgc agatgctgtc ggccacggcc agcgacgcct ccggcggcgg aggaggcatc
    14641 caggcgggcg acgtggtcca gatcattttc agcgaaaaca ccacatcccc gctcctcacc
    14701 agcgccaaca tcaacgcggt gttgtctctc agttccgcgc acacctggct ggacgcctcg
    14761 aacgccctgg ggtccgccgt ctgggagcaa cccaacatcc tcacggtgac cctgtcggcc
    14821 gccggcggcc cccccaccgt ggccgtggga gacaccctca ccctggacgg cgccaccgtg
    14881 aaagacgccg ccaacaacgt ttccgccggc agccgcgccg tcggcggcga cttcggctcg
    14941 gacagcacgc cgccggtcat cgcctcccgc gagacggcgg acctggattc ggacggctgg
    15001 atcgacgcca tccacgtgac gttcaacgag aagattctgg actccagcgt gaccgccgcc
    15061 gatttcgatg tggccgacgt gacgggggaa tcgttcgcct ccaccaccca cggagacacc
    15121 atcgacgacg cggacatcta catcacgttc acggacggcc tcctcggcac cgacgccacg
    15181 cccaccgtct cgttcgccag cgggaccttc tgcgaccttt caaacaacaa gatggcgtct
    15241 tcccccgcca ccccgtcgtc cgacagggcg ccgccggctc tcctgtcggc cgccgccgac
    15301 gacagcgcgt atctggcgac gggcatcgac ggggacgaca ccgtcaccct ggtcttcagc
    15361 gaaaaaacca acgcgcccac cgtcgacgcc tccaacatcg acgccgtcct cccgctttcc
    15421 ggcggacaca cctggaaaga cggcaacggc accgtcgggg gcgccgcctg gaacagtccc
    15481 gccaaaaata cgctgacggt gcaactgagc ggcggcgccg gcctccccac ggtcgccgtg
    15541 ggagacacgc tgagcatccc cgccggcctc atcgaggacg tgctggggaa caaaaccacc
    15601 gacgcggcgc tgatcggcgg aacgttcacg gggacggaca ccaccccgcc ccaaatcctt
    15661 tccgtttatc ccgaagacaa atccgcgaac acgtcggtga ccgccacccc gcgcgtacgg
    15721 ttcagcgaaa acatgaacgc ggactcggcc cggaccgctt tcacgctgca gatcacgcgg
    15781 gaccgcgacg gaaaaaaccg caacgctgcc gtgagcggcg ccaccgtctt ttcatcggcg
    15841 gacaacaccc tcacgttcac ccccgccgaa ccgctcgaaa gcggatcggc ctatcagatc
    15901 acggtgtcca cttccgccca ggacaccatc ggcaacccgt tggggacctc acacacctcg
    15961 cgcttccgca cgctgctcga ttccgcctcc agcaacctgg tggtggcgga ggacgaaaaa
    16021 agccgcgttt atctgccctc gggggcgacg acggtctccg tggccgtccg tatcggtctc
    16081 gatccggttg gtcagcccca acgcgtcgac ccgcagaaga tcgaggaagc caaccgaaag
    16141 atccggtccg acgcctatcg cgccaacgac ccgctgacgg gcgctctgcg tgaaatctgc
    16201 ctctacgatt cacaaggcgc cccgctggac ctccgtttcg cggtgccggc cacggtgtcc
    16261 atcccctaca cggagaccgg cggtttcgtg gacggcacca gcccgctcct gccggaagac
    16321 cggctgtcca tgtattgtct cgacgaaacg aacgccctct gggtgcgcat ccccgactcg
    16381 tcggtaaaca ccgccgacaa cctcgtgacc gcccctaccc cccatttcag cgtgttcggc
    16441 ctctttggaa acccccactc cggagggaac tccgacctct acgccttccc ggtgcctttc
    16501 cgtcgcgggc gggacagcgt ggtcacgttc gtgggactgc ccatcgacgc caccgtgaag
    16561 atccacaccc tttccggaac gcgcgtgcgc accctgcccc gcgcgggggg cgcccaggaa
    16621 acctgggacc tgaaaaacga cgacggcgac accgtggccg atggcgtcta tctctacgtg
    16681 gtggaaaacg gcgggttccg cgccacgggg aaacttttgg tgacgcgatg aagcaaaacg
    16741 gtttaaagtt taaggagaaa agtttaaggg gttgcctttg gccgattttc ttagccttta
    16801 ctttaaactt tgaactttta acttctaact tatcggcctc gtccggcggg gaaaccgggg
    16861 cgcaggtgtt gaccctgggc gcgggatcgc gggcgctggg catggcggaa gcctccgccg
    16921 gactggccga cgacgccttc gggctctttg gaaacgcggc ggggctcggt tttctgcgcg
    16981 acagcgaagt gtccgcggcc tatcatcctc tgggagagga catcgccaac cggtcggtgg
    17041 tctatgccca cccgttgtct tcgcggcccg gcgcctgggc cgtcgacggg tcctatctgg
    17101 acgtcaccga catccgcggg tactccccgg gcggcgagaa gacgggcgac gtgaaagcga
    17161 gcgatcttgt gtttggcgcg gcgtatggac ggccggtggc gaaccacctg gcggcggggc
    17221 tgaggttcaa aggcctgaga gaaaccctgg acggccgcac agcggccacc ttcgccgccg
    17281 acgccgggct cctctaccgg cccccccggg gcgcgggggc ggggctgagc gtgcagaatg
    17341 tgggtccggg ac
//
LOCUS       DAOTFD010000031        16728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2337_length_16728_cov_5.500510, whole genome shotgun sequence.
ACCESSION   DAOTFD010000031 DAOTFD010000000
VERSION     DAOTFD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_16728_cov_5.500510"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="P5079_03470"
     CDS             complement(<1..312)
                     /locus_tag="P5079_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRY29078.1"
                     /translation="MSRPMKWRVTDGWCRPQGKAASLNGFFIASSTRTLGDMARPRVF
                     DAALRRTGLTPRRWAAGEQVHGRSVRFVKRPTAPRRIPGVDGVATDVPGLVLQVHTAD
                     CV"
     gene            complement(309..1412)
                     /locus_tag="P5079_03475"
     CDS             complement(309..1412)
                     /locus_tag="P5079_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY29079.1"
                     /translation="MTATELFHELLKEMVERGASDMHIRAGNPPMVRILGDLEPSRRQ
                     EVLTPEVTKEIAFSIMNQRQRLIFESRFEVDLSVSVPEIGRFRGNVFQQRGQINIALR
                     IVPTAIPTIEELNLPVVVKNLCSRRRGLILVTGTTGCGKSTTLASMIDYINSERKAHI
                     ITIEDPIEFLHKDKKAVVSQRELGLDTTSYAEALKHVVRQDPNVILLGEMRDLETIAA
                     AVTSAQTGHLVLSTIHTIDAVQTISRIIDMYPPHQQNQIRLQLADSLQGVISQRLLRR
                     ADVPGRIPAIEVLVTSALVKKAIEDNNFAELYNAIRQGHYYGMQTFNQALVQLYNEGK
                     VRLDEALAAASNPEEVMLAVRGIETGVDATKSF"
     gene            complement(1454..2584)
                     /gene="ftsZ"
                     /locus_tag="P5079_03480"
     CDS             complement(1454..2584)
                     /gene="ftsZ"
                     /locus_tag="P5079_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY29080.1"
                     /translation="MVRIKFAEDFSEQPANIRVIGLGGAGGNAVNRMIEAGVSHVEFI
                     AANTDSQALRRSLAPVRIQLGERLSKGLGVGGNPVLGRQAAEESREHIREVLSGADMV
                     FITAGMGGGTGTGAASVVAEAVRSLSPTPLVVGVVTRPFEFEGLVRKNQADLGIKELR
                     SHVDTLLAIPNERLFEIIDEKTSALEAFRVADNVLRQAVQAITDVITRHGVINVDFND
                     VRSIMSGAGEALMGMGEGHGKDRALTAAKQAIHSPLLENVSIDGAKGVLVNVSGNEKV
                     TMMEVRNAMDYIKNAASPNAHVFYGHVMDNSLEDRLLVTVIATGFPPVRTLGGQKRRG
                     RDVAEAAVSHGGSRISPSGEVLPESDLRRPAYLRWKLRKLHG"
     gene            complement(2585..3826)
                     /gene="ftsA"
                     /locus_tag="P5079_03485"
     CDS             complement(2585..3826)
                     /gene="ftsA"
                     /locus_tag="P5079_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRY29081.1"
                     /translation="MAKSEIVVSLDVGSAQVACVLARRNPETETLEVLGGARRACKGL
                     KGGVVINIDETARAVSRVMEETEEMAGLSGAARNVLIGVRGAHIQTFNHHGAMNIART
                     DKEITAEDRDQVVENTKAVPISPDREIVHVIPQDFILDRQNGVPNPVGMEASLLEVDV
                     HIVTASQSHLNNIWKAIGRAGFQVEEPIYGLLAVGDVVVTAEEKDLGCLLVDLGGQTT
                     SLAIYAEGSVRFTKELPIGADAITHDLSHALRTSISQAQLVKERYGAATRDLADGDAD
                     EEIPYTSVDGRTPRTMKRTAIFDYIAPRIEEIFTMVSEELQNSNYAEHVSGGGVILTG
                     GGSLLTGIVPAAEQILELPVRQGLPQGIAGPPDVVGHPSYATALGLLHYRHLGEWSRS
                     RRASRKITLGQRVRAFVEDIF"
     gene            complement(3867..4700)
                     /locus_tag="P5079_03490"
     CDS             complement(3867..4700)
                     /locus_tag="P5079_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRY29082.1"
                     /translation="MPIKRHRVKLRKSYVRYRFKTAAQATWTWSRRLTFVALGVWAAQ
                     AGHRFWSQTPYLRVKAVQGAADVPAALLTETTVRAGDHLFRFSTRAIERHLQQRYPEF
                     SRVSVRRRPDGRVAVAVDRRVPVARVADGENWRGMAEDGVTFPLDETRFPREPLPVLA
                     VPGEGEAARPALAFLKLISSQDAAWVKELRKLKAEPDGEATLFLRADLPVHWGSMAES
                     PKVVRRKAQRLERVLNDDSLAEGAAHLRFIDEDRVAVKPLTAELLKKTADDNKNGPER
                     P"
     gene            complement(4734..5702)
                     /locus_tag="P5079_03495"
     CDS             complement(4734..5702)
                     /locus_tag="P5079_03495"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRY29083.1"
                     /translation="MNGCARRGDEPAGLQLLRRKKVGVLCGGWSAERPISLRSGAAVA
                     MSLRRMRIPHAVIDVTRRVSEQLRRARIDVAFLAMHGPYGEDGTLQGALEMMGLPYTG
                     SGVLASATAMHKPTAKRLFQSAGLPTPPWKIVGAGERNPKIPFSGPWVVKPASQGSAI
                     GITIVRRKSEWQKALRAAARLEKEVLVEKFVPGTEVTVAVLDQRCLPVIEIVPKHRYY
                     DFYSKYAAGGSRHIIPARLPRAVLKKVSELSLAAFRTLGCRHLARVDLIVPGQGRPQI
                     LEVNTLPGLTNTSLFPDAARATGMDFNHLILEMLRMSLRDAGRKTG"
     gene            complement(5719..6639)
                     /gene="murB"
                     /locus_tag="P5079_03500"
     CDS             complement(5719..6639)
                     /gene="murB"
                     /locus_tag="P5079_03500"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRY29084.1"
                     /translation="MSADDPSSLRTELASLAELVRENEPLARHSTFAIGGPADFYVEP
                     KTLPELKKIVAWSNRHGLPLFPIGQGSNLLIGDKGVRGVVVRLRGEFEEISFDGERVS
                     AGAGAPLPSLAKACAQNGLAGAEPLAGIPGTVGGALMTNAGTPEGDIGSLVDEVEILE
                     NSGKPAVLAKKDIVFSYRRSDLPGRLVLRARLKLRRGDKNDIMAAIQNQLNRRAKTQP
                     LGSYNVGSIFKNPPGDFAARLVDAAGLKGLRVGGAAVSDKHANFIVNAGQATAEDVHR
                     LIEKIRRTVQEKFGVLLETEIWFAGERSTF"
     gene            complement(6660..8036)
                     /gene="murC"
                     /locus_tag="P5079_03505"
     CDS             complement(6660..8036)
                     /gene="murC"
                     /locus_tag="P5079_03505"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRY29085.1"
                     /translation="MFKKIRHIHFIGIGGSGMSGIAEVLIVSGYRVSGSDMKETDVTR
                     RLKDIGADVRIGHKAENVAGAHVVVTSTAVGPTNPELTEALKRKIPVIPRIEMLAEVA
                     RLKYAITVAGTHGKTTTTSLTSLVLQAGGLDPTVVVGGRMHNIGTGARVGRGEFVVAE
                     ADESDGSFLKLSPTIAVVTNIDNDHLDYWGSMQPLYGAFEAYANKVPFYGAVILGVDD
                     PGSRRLLSRVRRTMITYGLTEDAEIRAVDLTVEPGKNSFVVKRKGERLGQVTWSVPGR
                     HNVINSLAAITVGLELGVSFKTIVEALVSLKGVGRRLEVKGEAGGVLVVDDYGHHPTE
                     IKATLQALKERWPGRRCLTVFQPHRFSRTKLLADDFAACFQGADRLFLLDVYSAGEKA
                     IEGVDSAWLANIISKKTPVTRVTSADAAAALTDAARPGDVLLTLGAGDVWKLGEGFLQ
                     TAAGPAKK"
     gene            complement(8357..8932)
                     /locus_tag="P5079_03510"
     CDS             complement(8357..8932)
                     /locus_tag="P5079_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29086.1"
                     /translation="MKIAFVLFCLSLAPPVWGGELFREANSPNEKMLSDIQKENAPSN
                     NKELAGFRGIAWGTSLQSISGLTEIKSGSSDVKEYTRNADEMQLGGAKLDRIIYRFWN
                     DQFFEVSLQTTGVINIQELKNVTFQKFGRMTQPNQFLEDYIKTMRTTQIFLLCPPVTD
                     AAFLTIRYRPLQKLREKAAKARAQEGADNGF"
     gene            complement(8938..10281)
                     /locus_tag="P5079_03515"
     CDS             complement(8938..10281)
                     /locus_tag="P5079_03515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRY29087.1"
                     /translation="MKKRTSPKKSAAAPAKKKPAEIKTAKPKPSDKLFPADTPPPDLP
                     AAPGPQTPPVEPEDTGKFKRIVLKPHEDRRVRRGHLWVFSNEVQSLPADVGAGDPVHF
                     YTAHKEFLGVGFYNPHSLIAGRLLDRHAVEPDAPFFEKRLRNALDLRRSLYSGDAYRW
                     VFGESDDLPGLVIDRYGDACVIESYCAGMDKLMPVILEAVNAIHPWKTVVLKNDVDAR
                     RYEKLSMEVKLVRGQADGPHWFEMDGLKIAADLTGGQKTGFFLDHRDNRAVVAAFGKG
                     KRVLDVFCHTGAFGLWAAQAGAASVTGVDDAEPALNLAREIADANALGERMNFVKANA
                     FEWLPSSKEKFDIIVLDPPKMAASKKNLPAAIQAYARLNAAALRRVSGGGFLATASCS
                     QHVEKDTFRQILSRAAYESGRRVRQIFWGGQAKDHPVRLSMPETDYLKFAILSVS"
     gene            complement(10283..10870)
                     /locus_tag="P5079_03520"
     CDS             complement(10283..10870)
                     /locus_tag="P5079_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HRY29088.1"
                     /translation="MSNETRPLPKNLFLTGTPGVGKTTLIKEVTLPYLDRVAGFYTEE
                     LKEGPERKGFWMKSFMPLSSGLSGGLLAKKGMKSPTKLGKYGVDMKILEGVGAAALSE
                     AMARSGTVLVIDELGSMEVLSDLFRKKLLECLQSKGHPVLATIRFNAQPFTGHVKRLA
                     DSALLRLTRENFVETKTEVRRWLEGQLLSNKSQGQ"
     gene            complement(10887..11609)
                     /locus_tag="P5079_03525"
     CDS             complement(10887..11609)
                     /locus_tag="P5079_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRY29089.1"
                     /translation="MPSRSPRGVVSEIFRSLQGEGIYVGEPQVFVRLAGCPLRCRYCD
                     TPQHLRRNGRTAMRVAEVLRRVRRLDAKKLCRTVSVTGGEPLMQAEFLTALLPALRRA
                     GRRTYLETSGVHPHLLRRVVRDCDVVAMDIKCPGDTGKPYWTEHREFLAVAGKKAFVK
                     IVLTSKTTEAEVRKAVELVADARPRPPLVLQPVTPVRDRRHGGALRPPSPERLAAIFK
                     YARRRLSDVRLVPQMHRLWGLP"
     gene            complement(11613..12290)
                     /gene="queC"
                     /locus_tag="P5079_03530"
     CDS             complement(11613..12290)
                     /gene="queC"
                     /locus_tag="P5079_03530"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRY29090.1"
                     /translation="MAADAVVLLSGGLDSSTALSWALKVKKWRCHCLVADYGQRHRRE
                     ITAALRVARRARCPVHRIKFRLPWGGSSLLNRRQALPVHAPSAIGRGRIPSTYVPARN
                     TIFLSFALSLADVLDAENIVVGANALDYSGYPDCRPKYYAAVQKTGRLGTRLGTEKKR
                     PLRVWAPLLHLTKKDIVRRGARWGTPYELTWSCYRGGARPCGRCDSCRLRARGFQQAG
                     LPDPLLG"
     gene            complement(12434..13240)
                     /gene="murI"
                     /locus_tag="P5079_03535"
     CDS             complement(12434..13240)
                     /gene="murI"
                     /locus_tag="P5079_03535"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRY29091.1"
                     /translation="MKNNRSPIGVFDSGVGGLTVLRALMRRLPHERFVYVGDTARVPY
                     GTKSPEAVKRFSLEIARFLKKRRVKAVVVACNTVSAVALPELRRFLPVPVMGVLEPGA
                     TAAARATRTGRVGVIGTSATVRSRAYEREIRRLNPALRVFSRPCPLFVPLVEEGWLTH
                     PVTRRVAQLYLSPILAKRVDALVLGCTHYPLLKTVLKSVARNVSLIDSAEETAKSVKR
                     VLERKDLLARSGRGSGVFFSSDDPDKFMALGHRFLGGKLRRVRRIHLDGV"
     gene            complement(13237..14856)
                     /locus_tag="P5079_03540"
     CDS             complement(13237..14856)
                     /locus_tag="P5079_03540"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY29092.1"
                     /translation="MRVPLTAGLAALWLAAAPCFVFGQARRPPLDQEKFTAVVDGKIR
                     TDLSTLHINAAPCLALGDVQRLFGGRQSWRRVSRRVSYEVSGRRMEFQLDASTAVVDG
                     RTVTLDFPALYWSGQVYLPVSFLVTKEFQRAVEAKTAWDPAARTLLVEPVPSVSSPRF
                     YSYPEKSRVVVELGPRVHFRVLGYRNNRLYVRFFGGRSLGNEKIAAEDGLVEWVQLIG
                     HARSSDLTLALSPEAGTPRVTQQETPRQMVIEVAAKKDPPVFSPSVSLPAPTPDPSAP
                     DADDALFLDEDLGGPTEPVSPLLALSPIKTIVVDAGHGGQDVGAVGPHGTLEKDVNLE
                     MAKALARVLKRERRFNVVLTRNDDTFIPLDERFEVANKHKADLFISIHSNAALSSRSN
                     GFEVYFLSEKATDDAAAAVARRENAVVEMEGVAGKTKQKLQELLWSLARTETMNESSE
                     IAALMLRQVGKRVRIPMRGAKQAGFVVLKGANMPAVLVESAFISHPKEEGLLRSRRFR
                     TKMADGLYAGILDYEKRKIQARAQKTAGKSQ"
     gene            14976..15902
                     /locus_tag="P5079_03545"
     CDS             14976..15902
                     /locus_tag="P5079_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY29093.1"
                     /translation="MTELNREKEISKLKADRQANEDELRLLFSFAKEVAFTRNVDVLL
                     DQVVGQAAKVLRAEAASLLLMNHETLQLDFKVVKGQNSEAIRNLDIHLRPGEGLAGWV
                     AASHQPAIVNNAEDDPRFKPEVDWLTGFHTRNLIAVPMMAQGKLIGVIEVVNRLDKKD
                     FTEHDVEFLGSVAWLAAMAFDNAQTYHALEYSRDQLTNIVESMPGGFIGVDMKSRITH
                     CNLRAQEILALPPNAVGQRFTQILAEQPGAVEAITRAFSENQGNKRQIFHSTTPFRGR
                     RQIGYSTLMIRSRHGELQGVGILFQDITDFAS"
     gene            complement(15899..>16728)
                     /locus_tag="P5079_03550"
     CDS             complement(15899..>16728)
                     /locus_tag="P5079_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY29094.1"
                     /translation="YWRWWVVHLWVEGFFEVFSTVVVAFLFSRMGLLTPYTATGSVLF
                     STIIYLSGGVIGTFHHLYFTGTPTAVLAFGAVFSALEVVPLVLIGFEAYDNLTLSRAK
                     PWVTAYRWPIYCFVAVAFWNLVGAGLFGFLINPPIALYYMQGLNTTPVHGHAALFGVY
                     GMLGIGLMLFCLKGLAARNVWKTKLLAFSFWSINIGLALMILLSLLPLGLLQTWASVE
                     KGMWFARSAEFLQSDLANTLKWMRVVGDTVFAAGALALGWFVLGLKTGWSVREGTDL"
BASE COUNT         2858 a         5243 c         5486 g         3141 t
ORIGIN      
        1 cacgcaatcg gccgtgtgca cctggagaac caggccgggg acgtcggtgg cgaccccatc
       61 cacgccgggg atccgccggg gcgcggtcgg ccgtttcaca aagcgcacag agcggccgtg
      121 gacctgttcg ccggccgccc agcgccgggg cgtcagtccg gtccgccgga gcgccgcgtc
      181 gaacacacgg gggcgggcca tgtcgcccag ggtccgcgtg ctggaggcga tgaaaaaacc
      241 gttcagcgac gccgccttgc cttgcgggcg gcaccagccg tccgtcaccc gccacttcat
      301 cgggcggctc aaaaagattt cgtggcgtcg accccggtct cgatgccccg caccgccagc
      361 atgacctctt cggggttcga ggccgcggcc agggcctcgt ccagccggac cttgccctcg
      421 ttgtagagct gcaccagggc ctggttgaac gtttgcatgc cgtagtagtg tccctgccgg
      481 atggcgttgt agagttcggc gaaattgttg tcttcaatgg cttttttcac cagcgcgctg
      541 gtgaccagca cctcgatggc ggggatgcgt cccggcacgt cggcccgacg gagaagccgt
      601 tgggagatga cgccctggag cgaatccgcc agttggagcc gtatctggtt ctgttggtgc
      661 ggcggataca tgtcgatgat gcggctgatg gtctgcaccg cgtcgatggt gtggatggtg
      721 gagagcacca ggtggcccgt ctgggccgag gtgacggccg cggcgatggt ttccaggtcg
      781 cgcatttcgc ccagcaggat cacgttgggg tcctggcgca ccacgtgctt gagcgcctcg
      841 gcgtagctgg tggtgtccaa ccccagttcg cgctggctga cgacggcttt cttgtccttg
      901 tgaaggaact cgatgggatc ctcgatggtg atgatatggg ctttcctctc ggagttgatg
      961 tagtcgatca tggacgccag cgtggtggat tttccgcagc cggtggtgcc ggtcaccagg
     1021 atcagcccgc gccggcggga gcagaggttc ttcaccacca cgggcaggtt cagttcctcg
     1081 atggtgggga tggcggtcgg cacgatgcgc agggcgatgt tgatctggcc gcgctgctgg
     1141 aagacgttgc cgcggaaccg gccgatctcc ggcaccgaga cggagaggtc cacttcgaag
     1201 cgcgactcga agatgagacg ctggcgctgg ttcatgatgg agaaagcgat ctccttggtg
     1261 acctcgggcg tgaggacctc ctgccggcgg ctgggttcca ggtcgccgag gatgcgcacc
     1321 atgggcgggt tgcccgcgcg gatgtgcatg tcggaggcgc cgcgctcgac catctccttg
     1381 aggagttcgt ggaacagttc cgtggcggtc attggggcat cccctttaat agattctcgg
     1441 tgcggcgccg gtttcagcca tggagcttcc gcagtttcca acggaggtag gccggccgtc
     1501 gcaggtcgct ttccggcagc acttcgcccg agggagaaat gcgcgagccg ccgtggctca
     1561 ccgccgcctc cgccacgtcc cgtccgcggc gtttctgtcc cccgagggtc cttaccggag
     1621 gaaacccggt ggcgatgacc gtgaccagga ggcggtcctc cagggagttg tccatcacgt
     1681 gcccgtagaa cacgtgcgcg ttggggctgg cggcgttctt gatgtaatcc atggcgttgc
     1741 gcacctccat catggtgacc ttctcgttgc cgctgacatt gacgagaaca cccttggcgc
     1801 cgtcgatgga gacgttttcc agcagcggcg agtggatggc ctgtttggcc gcggtgagag
     1861 cgcggtcctt gccgtgcccc tcccccatgc ccatcagcgc ctcgccggcg ccggacatga
     1921 tggaacgcac gtcgttgaag tccacgttga tcacgccgtg ccgcgtgatg acgtcggtga
     1981 tggcctgcac cgcctgtcgg aggacgttgt cggccacgcg gaaggcctcc agcgcggatg
     2041 ttttttcgtc gatgatctca aaaagccgtt cgttggggat ggccagcagg gtgtccacgt
     2101 gcgagcgcag ttccttgatg ccgaggtcgg cctggttctt ccgcaccagc ccctcgaact
     2161 cgaaggggcg cgtgaccacg cccaccacca ggggcgtggg cgaaaggctg cgcaccgcct
     2221 cggcgaccac cgaggcggcg ccggtccccg tgccgccgcc catgccggcg gtgatgaaga
     2281 ccatgtccgc cccggagagg acttcgcgga tgtgttcccg gctctcctcc gccgcctggc
     2341 ggccgaggac ggggtttccc cccacgccga gccccttgct gagtcgttcg ccgagctgta
     2401 tccgcaccgg ggccaggctg cgtcgcaggg cctgggagtc ggtgttggcg gcgatgaact
     2461 ccacgtgcga cacgcccgcc tcgatcatgc ggttcacggc gttgccgccg gcgccgccca
     2521 ggccgataac ccggatgttg gccggctgct cgctgaaatc ttcggcgaac tttatgcgga
     2581 ccatttagaa gatatcttcg acgaaggcgc gcacgcgctg gccgagggtg atcttgcgcg
     2641 acgcccgccg cgatcgcgac cattcgccca ggtgccggta atgcaggagg cccagcgccg
     2701 tggcgtagga tgggtggccc accacgtcgg gcgggccggc gatgccctgc ggcagtccct
     2761 gccgcaccgg cagttccagg atttgttccg cggccggcac gatgcccgtc aggagggacc
     2821 cgccgccggt gaggatgacg ccgccgccgg agacgtgctc cgcgtagttg gagttctgca
     2881 gttcctcgga aaccatggtg aatatttcct cgatgcgcgg ggcgatgtaa tcgaaaatgg
     2941 cggtgcgttt catggtgcgc ggcgtgcggc cgtccacgct ggtgtagggg atctcctcgt
     3001 cggcgtcgcc gtcggccagg tcgcgcgtgg cggcgccgta gcgttccttc accagctgcg
     3061 cctgcgagat ggaggtccgc agcgcgtggg agaggtcgtg cgtgatggcg tcggcgccga
     3121 tggggagttc cttggtgaaa cgcacgcttc cttcggcgta gatggccagg ctggtggtct
     3181 gtccgccgag gtccaccagc aggcatccca gatctttttc ttcggccgtg accaccacgt
     3241 ctcccacggc gagaaggccg tagatcggct cctccacctg gaaccccgcc cggccgatgg
     3301 ctttccagat gttgttgaga tggctctgag aggcggtgac gatgtgcacg tccacctcca
     3361 ggaggctggc ctccatgccg acggggttgg gcacgccgtt ctggcggtcg aggatgaaat
     3421 cctgcgggat gacgtggacg atctcgcggt cgggggagat gggcacggcc ttggtgtttt
     3481 ccacgacctg gtcgcggtcc tcggcggtga tctccttgtc ggttcgggcg atgttcatgg
     3541 cgccgtggtg gttgaaggtc tggatgtgcg cgccgcgcac gccgatgagc acgttgcgcg
     3601 ccgcgcccga aaggccggcc atctcctcgg tctcctccat cacgcgggac acggcccggg
     3661 cggtctcgtc gatgttgatg accacgccgc ctttcaggcc cttgcaggcg cgtcgggcgc
     3721 cgccgaggac ttcgagggtc tcggtttcgg ggttgcgccg cgccagcacg caggcgacct
     3781 gcgcgctgcc gacgtccaga cttacgacga tttcagattt tgccatagct caaatcctcc
     3841 ggggacgttt ttaaccggcg ttcctctcag ggtctctcgg ggccgttctt gttgtcgtcg
     3901 gcggtttttt tcagcagttc cgccgtcaaa ggttttaccg ccacgcggtc ctcgtcgatg
     3961 aaccgcaggt gcgccgcccc ctcggccaac gaatcatcgt tcaagacgcg ttccagccgc
     4021 tgggcttttc gccggaccac cttcggggat tccgccatcg agccccaatg cacgggcagg
     4081 tcggcgcgga ggaaaagggt cgcttcgccg tcgggctccg ctttgagttt gcgcaactct
     4141 ttcacccagg ccgcgtcctg ggaagagatc aatttcaaaa aagccaacgc cggccgggcc
     4201 gcctcgcctt ctcccgggac cgccagcacg ggcaggggtt ccctcggaaa acgggtttcg
     4261 tccaagggaa acgtcacgcc gtcttcggcc atgccgcgcc agttttcgcc gtcggccacg
     4321 cgcgccaccg ggacgcgccg atccacggcc acggccaccc ggccgtccgg ccgacgtcgc
     4381 accgacacgc gggaaaattc aggataacgt tgctgcaggt gccgttcgat ggcccgcgtg
     4441 gagaaccgga aaagatggtc gccggcccgg acggtggttt ccgtcaggag cgcggccggc
     4501 acgtccgccg cgccctgcac ggctttcacg cgcagatacg gcgtctggct ccagaaccgg
     4561 tggcccgcct gcgccgccca cacgccgagc gccacgaacg tcagccgccg gctccacgtc
     4621 caagtcgcct gcgccgcggt tttgaaccgg taccgcacat aactcttccg cagtttgacc
     4681 cggtgacgtt tgatcggcat ttttcccggc gtccgaggtc cccgtggggg cggtcagccg
     4741 gtttttcggc cggcgtcccg gagggacatc cgcagcattt caaggatcag atggttgaaa
     4801 tccatccccg tggcgcgggc ggcgtccgga aagagggacg tgttcgtcag gcccggcagg
     4861 gtgttcactt ccagtatctg ggggcgaccc tggcccggca cgatcaagtc cacccgcgcc
     4921 aagtgacggc acccgagggt ccggaaggcg gccagggaca gctcggagac ttttttgagc
     4981 acggcccgcg gcaatcgcgc agggatgatg tgccgcgaac cacccgcggc gtatttcgaa
     5041 tagaaatcgt aatagcggtg tttgggaacg atttcgatca ccggcaggca tcgctggtcc
     5101 agcacggcca ccgtcacttc cgtgccgggg acgaacttct ccaccaggac ctctttttcg
     5161 agccgggcgg cggcgcgcaa cgctttttgc cattcggatt tccggcggac gatggtgatg
     5221 ccgatggccg aaccctggga ggcgggtttc accacccacg ggccggaaaa ggggatcttc
     5281 gggttccgct cgccggcacc gacgattttc cagggcggcg tgggaagtcc cgccgattga
     5341 aagagccgtt tggcggtggg tttgtgcatg gccgtggcgc tggccagcac tccgctgccg
     5401 gtgtaaggaa gccccatcat ttccagcgcg ccttggaggg tgccgtcttc cccgtagggg
     5461 ccgtgcatcg ccagaaaagc cacgtcgatg cgcgcccggc ggagctgctc ggacacgcgg
     5521 cgggtgacat cgatgaccgc gtgcggaatc cgcatccgcc gcagggacat ggccaccgcg
     5581 gcgccgctgc gcagggagat gggccgttcc gccgaccatc ctccgcagag gaccccgact
     5641 ttttttcgac gaagaagctg caatcccgcg ggttcgtccc cgcggcgggc gcatccgttc
     5701 acgtttccga ccgccttttc aaaaagtcga gcgttcgccg gcgaaccaga tctccgtctc
     5761 caggagtact ccgaactttt cctggaccgt ccgacggatc ttttcgatca accggtgcac
     5821 gtcttccgcg gtggcctgcc ccgcgttgac gatgaaattg gcgtgcttgt cggacaccgc
     5881 ggccccgccc acgcgcaaac ccttcaaccc ggcggcatcc accaggcgcg cggcgaaatc
     5941 tcccggcggg ttcttgaaga tggaacctac attgtaacta cctaaaggct gcgttttagc
     6001 gcgccgattc agctggttct gaatggcggc cataatatca tttttgtccc cccgtcgcaa
     6061 tttgagccgc gcgcgcaaga ccagccggcc gggcaggtcg ctccgacggt aagagaagac
     6121 aatgtctttc ttcgccagca ccgcgggttt ccccgaattt tcaaggattt ccacctcgtc
     6181 cacgaggctt ccgatgtccc cctcgggcgt gccggcgttg gtcatgaggg ccccccccac
     6241 ggtgccgggg atgccggcca acggttccgc cccggccagc ccgttctggg cgcaagcttt
     6301 ggccaaagac ggcagcgggg cgcccgcgcc ggcggagacg cgctcgccgt caaatgagat
     6361 ttcttcgaac tccccgcgca ggcggaccac cacgccgcga acgcctttgt cgccgatgag
     6421 gaggttggag ccctgtccga tggggaacag ggggagtccg tggcggttcg accaagcaac
     6481 gatttttttc agttcgggaa gggttttggg ctcgacgtag aaatcggccg ggccgccgat
     6541 ggcgaaggtg gagtggcgtg cgagcggttc gttttcgcgg acgagttcgg cgagagaggc
     6601 cagttcggtc cgaagggagg acgggtcgtc ggcggacatg gacggatcgg tcccggccgc
     6661 tattttttgg ccgggcccgc ggcggtctgg aggaaacctt cccccagttt ccacacgtcg
     6721 ccggcgccca gggtcaggag cacgtcgccc gggcgggcgg cgtcggtgag cgccgccgcg
     6781 gcgtccgccg acgttacccg ggtgacgggc gtttttttgg aaatgatgtt cgccagccag
     6841 gcgctgtcca cgccctcgat ggctttttcg ccggcggaat acacgtccag aaggaacagt
     6901 cggtccgcgc cctggaaaca ggcggcaaaa tcgtccgcca acagtttggt gcggctgaaa
     6961 cggtgcggct gaaacaccgt aaggcatcgc cggcccggcc agcgttcttt cagcgcctgc
     7021 agggtggcct tgatctccgt cgggtgatgg ccgtagtcgt ccaccaccag aaccccgccg
     7081 gcctcgcctt tgacttccag ccgccggccc acgcccttca aggagaccag cgcctccacg
     7141 atggttttga aggagacgcc gagttccaga ccgacggtga tggcggcgag ggaattgatc
     7201 acgttgtgcc ggcccggcac ggaccaggtc acttggccga ggcgttcgcc tttccgtttc
     7261 accacgaagg agttctttcc cggttccacg gtcagatcca cggcgcggat ttcggcgtcc
     7321 tcggtgaggc cgtaggtgat catggtccgg cgcacccggg agaggagccg ccgggacccc
     7381 ggatcgtcca cgcccaggat caccgcgccg tagaacggca ctttgttggc gtacgcctcg
     7441 aaggcgccgt aaagcggctg catgctgccc cagtaatcca gatggtcgtt gtcgatgttg
     7501 gtgaccaccg cgatggtggg cgagagtttc aggaaggaac cgtcggactc gtccgcctcg
     7561 gccaccacga attcgccgcg gcccacgcgc gcgccggtgc cgatgttgtg catgcggccg
     7621 cccaccacca cggtggggtc gagcccgccc gcctgaagga ccagcgaggt gagcgaggtg
     7681 gtggttgttt ttccgtgcgt gccggccacg gtgatggcgt atttgagccg cgccacctcc
     7741 gccagcatct cgatgcgcgg gatcacggga attttccgtt tgagcgcctc ggtcagttcg
     7801 gggttcgtcg gtcccacggc cgtggaggtg accaccacgt gggcgccggc gacgttctcc
     7861 gctttgtggc cgatgcgcac gtccgcgccg atgtctttga gccgccgggt gacgtccgtt
     7921 tctttcatgt cgctgccgga gacgcggtac ccggagacga tcagcacctc ggcgatgccg
     7981 ctcatgccgg agccgccgat gccgatgaag tggatgtggc ggattttttt gaacatggtt
     8041 atccttttgt cattgcgaac ccccgaattt cggggtgaag caatcgaact tttttgccgg
     8101 aatcggaaaa cgtcgcaggg cttcccgccc tgcacccggg tcgtttttct ttgctcgccc
     8161 aaagaaaaac gacgaaaaga aatgcgcccc ggcgggcaat cgctcaacga ttttcggcgg
     8221 ccgcgaactc agcattctta aagtcccatc gaacgcttcg gacatcgcgg cctgcccgcg
     8281 ccgaaaatcg tttcgttctt aagcgcccgc aaggggggca ataacttctt tagattctga
     8341 cgtatcgctg tggacttcaa aaaccgttat ctgcgccttc ttgtgcacgt gcctttgcgg
     8401 ccttctctcg aagtttttgg agaggacgat aacgaattgt cagaaaggca gcatcggtaa
     8461 ctggagggca gagcagaaat atctgggttg ttctcatggt tttaatgtaa tcctccaaaa
     8521 actgatttgg ttgagtcatg cgcccgaatt tttgaaacgt tacatttttc aattcttgaa
     8581 tgttaataac accggtcgtt tgaagtgata cctcgaaaaa ttgatcattc caaaagcgat
     8641 agatgatgcg atctaatttg gctccgccga gctgcatctc gtcggcgttc cgtgtgtatt
     8701 ctttaacatc ggacgacccg cttttgattt cagtcaatcc ggaaattgat tgaagcgatg
     8761 ttccccacgc tattccccga aatccggcga gttctttatt gtttgaagga gcattttctt
     8821 tttgaatgtc cgacagcatt ttttcgttgg gtgagttcgc ctcccgaaaa agctcaccac
     8881 cccaaacggg tggcgccagc gataagcaaa acaacacgaa tgcaattttc ataagtttca
     8941 gctcacgctt aaaatggcga acttcaggta gtccgtttcg ggcatggaca ggcggaccgg
     9001 gtggtctttc gcctggccgc cccagaaaat ttgccgtacc cgccggccgg actcatacgc
     9061 cgcccgtgaa aggatctggc ggaaggtgtc tttctccacg tgctgggaac aggacgccgt
     9121 ggccaggaac ccccctccgc tcacgcgccg gagcgcggcg gcgttcagcc gcgcataggc
     9181 ttggatggcc gccggtaaat tcttcttgct cgccgccatt ttcggcggat cgagaacgat
     9241 gatatcaaat ttctccttcg acgagggcag ccactcgaag gcgttcgcct tcacgaaatt
     9301 catccgctcg ccgagcgcgt tggcgtcggc gatttcccgc gccaggttca gggccggctc
     9361 ggcgtcgtcc acccccgtca ccgaggcggc cccggcctgg gccgcccaga gcccgaacgc
     9421 ccccgtgtgg cagaacacgt ccaacacgcg tttacccttg ccgaaggcgg cgaccacggc
     9481 ccggttgtcc cgatggtcta aaaagaaacc ggttttttgt ccgccggtga ggtccgcggc
     9541 gatcttgagg ccgtccatct cgaaccagtg cgggccgtcg gcctggccgc ggacgagttt
     9601 gacttccatg gacagttttt cgtaacggcg ggcgtcgacg tcgttcttca acacgacggt
     9661 tttccagggg tggatggcgt tgacggcctc aaggatgacc ggcatgagtt tgtccatgcc
     9721 ggcgcaatag gattcgatca cgcaggcgtc gccgtaacgg tcgatgacca gaccgggcag
     9781 gtcgtccgat tcgccgaaca cccaccggta cgcgtcgccg gaatagagcg aacggcgcag
     9841 gtcgagggcg ttgcgcaggc gtttttcgaa aaatggggcg tccggttcca cggcgtggcg
     9901 gtcgagaagg cggccggcga tgagcgagtg cgggttgtaa aagccgaccc ccaggaattc
     9961 tttgtgggcc gtgtaaaaat ggacgggatc tccggcgccc acgtccgcgg ggagggactg
    10021 gacttcgttg gaaaacaccc aaaggtgccc gcgccggacg cggcgatcct cgtgtggttt
    10081 gagcacgatg cgtttgaatt ttcccgtgtc ctcgggttcg accggcgggg tttgcgggcc
    10141 cggggcggcc gggagatcgg gcggcggggt gtcggcgggg aagagtttgt cggagggttt
    10201 gggtttggct gttttgattt cggccggttt tttcttcgcc ggcgccgcgg ccgatttttt
    10261 gggagacgtt ctttttttca ttttattgtc cttggctttt attggaaagg agttggccct
    10321 caagccagcg ccgaacctcg gttttggttt cgacaaaatt ttcccgcgtc agacggagca
    10381 gggcgctgtc ggccaggcgt ttcacgtggc cggtgaacgg ctgggcgttg aatcggatgg
    10441 tggccagcac cgggtgcccc ttcgattgaa ggcactccag aagttttttt cggaaaaggt
    10501 ccgacaggac ctccatggaa ccgagctcgt cgatgaccag caccgttccc gaccgggcca
    10561 tcgcttccga cagcgcggcg gcgcccacgc cttccagaat tttcatgtcc accccgtatt
    10621 tgccgagttt cgtggggctc ttcatccctt ttttcgccaa caggccgccg gaaaggcccg
    10681 acgacagcgg catgaacgat ttcatccaaa accctttgcg ttcgggccct tctttgagct
    10741 cttcggtgta gaagccggcc acgcggtcga gataaggaag ggtgacctcc ttgatcagcg
    10801 tggttttccc caccccgggc gtgccggtga ggaaaaggtt tttaggaaga ggcctcgttt
    10861 cgttcgacat tgaaacatcc ccccttttac ggcaggcccc acagccggtg catctgcggc
    10921 acgaggcgca cgtccgacag ccgccgccgg gcgtatttga aaatggccgc aagccgttcg
    10981 ggggacggcg gccgcagggc ccctccgtgt cgccggtccc gcaccggcgt caccggttga
    11041 agcaccaacg gcggtctggg ccgggcgtcg gcaacgagtt ccacggcttt ccgcacctcc
    11101 gcctcggtcg tttttgacgt cagcacgatc ttcacgaaag cttttttccc ggccacggca
    11161 aggaactcgc ggtgttcggt ccaatagggt ttcccggtat cgccgggaca cttgatgtcc
    11221 atggccacca cgtcgcagtc gcgcaccacg cggcgtagga ggtggggatg gaccccgctg
    11281 gtttccagat acgttcgccg gccggcgcgc cggagggccg gtaaaagggc cgtcaggaac
    11341 tccgcctgca tcaacggttc cccgccggtg acgctgacgg tgcggcataa ttttttggcg
    11401 tccaagcgcc ggacgcggcg aagcacctcc gccacgcgca tcgccgtccg gccgttccgg
    11461 cggagatgtt gcggcgtgtc gcagtagcgg caccgcaggg gacagcccgc gaggcggaca
    11521 aagacctggg gttccccgac gtagatccct tcgccctgga gggaacggaa aatttccgac
    11581 accacgcctc tgggcgaacg ggacggcatg cgtcagccga gcagcggatc cggaaggccc
    11641 gcttgctgga acccccgcgc tctcaggcgg caggagtcgc accggccgca gggccgggcg
    11701 ccgccgcggt agcaggacca cgtcaattca tacggcgtgc cccaccgggc gccgcgccgc
    11761 acgatgtctt ttttcgtcag gtgcaaaagc ggggcccaga cgcgcagggg ccgttttttt
    11821 tccgtgccca accgggtgcc gagccggccg gttttctgca cggcggcgta gtatttgggg
    11881 cggcagtcgg gatagccgga atagtccagg gcgttcgccc cgaccacgat gttctccgcg
    11941 tccaagacgt ccgcgaggga aagcgcaaac gagagaaaaa tggtgttgcg ggccggcacg
    12001 taggtggaag ggatccggcc ccggccgatc gccgacgggg catggacggg cagggcctgt
    12061 cggcggttca agaggctcga cccgccccag ggcaggcgga atttgatccg atggacggga
    12121 cagcgcgccc gccgggcgac ccggagggcc gccgtgatct ctcggcggtg tcgctggccg
    12181 tagtccgcga cgaggcagtg gcagcgccat tttttgactt tgagagccca ggaaagcgcg
    12241 gtggaggagt ccagccctcc ggagaggagc acgacggcgt cagcggccat tgaaagaggc
    12301 agaaaagtga gaagttgaag gtttaaagtt tagagggtcc gccgggaaaa tcaccgccgt
    12361 tgtaattgtg tttgacacgg cacgcctttc tttttaactt tcaactttaa actttgaacc
    12421 gcggtgcgtt ctgttacacg ccgtccagat ggatgcgtcg cacccggcgg agctttccgc
    12481 ccagaaaccg atggcccagc gccatgaatt tgtcggggtc gtcggacgag aaaaagacgc
    12541 cgcttccccg cccgctccgc gccaggagat ctttccgttc cagaacccgt ttcacggact
    12601 tggccgtttc ctcggcggaa tcgatgagcg agacgttgcg ggccacgctt ttcaggacgg
    12661 ttttcaaaag cgggtaatgc gtgcagccca agaccagggc gtccacgcgt ttggcgagga
    12721 tcggcgagag atagagttgc gccacgcggc gcgtcaccgg atgcgtgagc cagccctcct
    12781 ccaccagcgg cacgaacagc ggacacgggc ggctgaacac gcgtagcgcc gggttgagcc
    12841 ggcggatctc ccgctcgtag gcccggctgc gcacggtggc cgaggtgccg atgacgccca
    12901 cgcggcccgt gcgggtggcg cgggccgccg cggtcgcccc cggctcaagc acgcccatca
    12961 cgggcacggg gaggaaccgg cgcagttcgg gcagggccac ggccgagacg gtgttgcagg
    13021 ccaccaccac cgccttcacg cggcgtttct tgaggaatcg ggcgatctcc agggaaaatc
    13081 gtttcacggc ctccggcgat ttcgtgccgt aagggacgcg cgcggtgtcg cccacgtaga
    13141 cgaaacgttc gtgcggcagt cggcgcatca gggcgcgcag gacggtgagc ccgccgacgc
    13201 ccgagtcgaa caccccgatg ggagaacggt tgtttttcac tgggatttcc cggcggtttt
    13261 ttgcgcgcgg gcctggatct tccgtttttc gtagtcgagg atgcccgcgt aaaggccgtc
    13321 ggccatttta gtgcggaacc gccgcgaacg cagcagcccc tcttccttgg gatgcgagat
    13381 gaacgcggac tccaccagca ccgccggcat gttggccccc ttgagcacca cgaaccccgc
    13441 ctgtttggcc ccgcgcatgg ggatgcgcac gcgtttcccc acttgccgga gcatcagcgc
    13501 cgcgatctcg gaggattcgt tcatggtctc ggttcgggcg agggaccaca gcagttcctg
    13561 gagtttctgt ttcgtctttc cggcgacgcc ctccatctcg accacggcgt tttcccggcg
    13621 ggccaccgcg gcggcggcgt cgtcggtggc tttttccgaa aggaagtaga cctcgaaccc
    13681 gttcgaccgg ctggacaggg cggcgttgga atggatcgag atgaaaaggt cggccttgtg
    13741 cttgttggcg acctcgaaac gttcgtcgag ggggatgaag gtgtcgtcgt tgcgggtgag
    13801 gaccacgttg aaacgccgtt cgcgtttcag cacgcgggcc agggccttgg ccatttccag
    13861 gttgacgtct ttttccaggg tgccgtgcgg gccgaccgcg ccgacgtcct gtccgccgtg
    13921 gccggcgtcg acgacgatgg tcttgatggg ggagagcgcc aagagcggag acaccggctc
    13981 cgtcgggccg cccaaatcct cgtccaagaa cagggcgtca tcggcgtccg gggcggaggg
    14041 gtcgggcgtc ggcgccggga gcgagacgga gggagagaac accgggggat cttttttggc
    14101 ggcgacctcg atgaccatct ggcggggggt ctcttgttgg gttacccgcg gcgtgcccgc
    14161 ttcgggggag agcgccaggg tcaggtcgct gctgcgggcg tgtccgatga gctggaccca
    14221 ttccaccagg ccgtcctcgg cggcgatctt ttcgttgccg agggaccgtc cgccgaagaa
    14281 ccggacgtag agtcggttgt tgcggtaacc caatacgcgg aaatgcaccc gcgggcccag
    14341 ctccaccacc acccggcttt tttccggata ggaatagaag cgcggcgagg agacgctcgg
    14401 caccggttcg acgaggaggg tccgcgcggc cgggtcccag gcggtcttgg cctccaccgc
    14461 cctctggaat tctttggtca cgaggaacga caccggcaaa tacacctggc ccgaccagta
    14521 cagggcggga aaatcgaggg tcaccgtccg gccgtccacc accgcggtgg aggcgtcgag
    14581 ctggaactcc atgcgccggc cggagacctc gtagctcacc cgccgggaga cccgccgcca
    14641 cgactgccgt ccgccgaaga ggcgctgaac gtcccccagg gccaggcagg gggcggcgtt
    14701 gatgtggagc gtggagaggt cggtccggat tttgccgtcc accaccgccg tgaatttttc
    14761 ctgatccagc ggcggccggc gggcctgccc gaaaacgaaa cagggggcgg cggcgagcca
    14821 gagggcggcg agtccggcgg tcaaaggaac tctcatagtg acgactagta tatataatac
    14881 gttcaccggc gcgcaaggcg gttttccgac ggaaaacttg ccgcccgcgg aggattgaca
    14941 cgccgccgag ggtgtgttaa cattcgactc gtaccatgac ggaactcaat cgcgaaaaag
    15001 aaatttcgaa actgaaagcc gatcggcagg cgaacgagga cgaactccgg ctcctgttct
    15061 ccttcgccaa ggaggtggcg ttcacgcgca acgtggacgt cctcctcgac caggtggtcg
    15121 gccaggccgc caaggtcctc cgcgccgagg cggcgtccct cctcctcatg aaccatgaga
    15181 ccctccagct cgatttcaag gtggtgaagg ggcagaactc cgaagccatc cggaacctgg
    15241 acatccacct ccgcccgggc gagggcctgg ccggctgggt ggccgcctcc catcagcccg
    15301 ccatcgtcaa caacgccgag gacgacccgc gtttcaaacc ggaggtggac tggctgacgg
    15361 gtttccatac ccgcaacctc atcgcggttc ccatgatggc ccagggaaaa ctcatcggcg
    15421 tcatcgaggt ggtcaaccgc ctggacaaga aggatttcac cgaacacgac gtggagttcc
    15481 tcggctcggt ggcctggctg gccgccatgg ccttcgacaa cgcgcagacc tatcacgcgc
    15541 tggaatattc cagggaccag ttgacgaaca tcgtggagag catgccgggc gggttcatcg
    15601 gggtggacat gaagagccgc atcacccact gcaacctgcg ggcgcaggaa atcttggcgt
    15661 tgccgccgaa cgccgtcggg cagaggttca cccagatatt ggcggagcag cccggggcgg
    15721 tggaggccat cacccgggcg ttttcagaga atcaggggaa caaacggcag atattccaca
    15781 gcaccacgcc ctttcggggc cggcgccaga tcggctattc caccctcatg atccgcagtc
    15841 gccacggcga actgcagggg gtgggcatcc tctttcagga catcaccgac ttcgcttcct
    15901 aaaggtcggt cccttcccgc acggaccagc cggttttcaa tcccaacaca aaccatccca
    15961 gcgccagcgc ccccgcggcg aagaccgtgt cccccaccac gcgcatccat ttcagggtgt
    16021 tggcgaggtc cgactggagg aattcggccg agcgggcgaa ccacatgccc ttctccacgc
    16081 tggcccaggt ctgaaggagc ccgagcggca acaggctcag caggatcatc aacgccagcc
    16141 cgatgttgat ggaccaaaag gagaacgcca ggagtttcgt tttccacacg ttccgcgccg
    16201 ccagcccttt caggcagaac agcatgagcc cgatccccaa catgccgtag accccgaaca
    16261 gcgcggcgtg gccgtgcacc ggcgtagtgt tgagcccctg catgtagtag agcgcgatcg
    16321 gcgggttgat gaggaacccg aacagtcccg cccccaccag attccagaac gccaccgcca
    16381 cgaaacagta gatgggccag cggtaggccg tgacccacgg cttggcccgg ctgagggtga
    16441 ggttgtcgta cgcctcgaag ccgatgagca ccagcggcac cacctccagc gcgctgaaca
    16501 ccgcgccgaa cgccagcact gcggtgggcg tgccggtgaa gtagagatgg tggaaggtgc
    16561 cgatgacgcc gcccgacaga tagatgatgg tcgagaaaag caccgacccc gtggcggtgt
    16621 acggcgtcaa caggcccatc cgggagaaca ggaacgccac caccaccgtg gagaacacct
    16681 cgaagaagcc ctccacccac aagtgcacta cccaccagcg ccagtatt
//
LOCUS       DAOTFD010000032        16402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2396_length_16402_cov_4.594910, whole genome shotgun sequence.
ACCESSION   DAOTFD010000032 DAOTFD010000000
VERSION     DAOTFD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2396_length_16402_cov_4.594910"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1353)
                     /locus_tag="P5079_03555"
     CDS             complement(262..1353)
                     /locus_tag="P5079_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY29095.1"
                     /translation="MPEIKRIAVNTGGGDAPGLNAVIRAVVLSATRLGWEVLGIKRGY
                     DGLLDPSQVRPLTEEDVRGITAMGGTILGTTNKGNPFEYPMKQPDGSVKAVDRSAEVV
                     SNFKKLGLDALVAIGGDGSLRIAKRFNDLGVPVVGVPKTIDNDLQSTVVTFGFDTAVG
                     IATDALDKLHSTAESHRRVMVVEVMGRYAGWIALNAGVSSTSDVILIPEIPFDIEKVC
                     EKIRDREARGREFSIVVVAEGAVPKGGSMSTVGAKEVGREVRLGGICAAVAAEIEKRT
                     GKETRSLVLGHLQRGGGPTTFDRLIALRFGAAAVRAIQEGQFGGMVALNPPTVTLVPL
                     AEAVAKMKQVPLDCDTIKTARSLGVCLGD"
     gene            complement(1411..3225)
                     /locus_tag="P5079_03560"
     CDS             complement(1411..3225)
                     /locus_tag="P5079_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY29096.1"
                     /translation="MLTVKGIHKAYGAQVVFDGASLQMNESDRLAVVGPNGAGKSTLF
                     RMILGETPVEGGEIIRRRGLRLGHLPQETAPFGGGTVLEEALSSDREESASVDERRSA
                     EAKKVLMGLGFRLEDFSRPVAQLSGGWMMRVAIARLLVQKPDVLLLDEPTNHLDLESL
                     LWFQEYLRKAKCGLLLISHDRAFVNALVHAVVELRDHKLFRYPGDFDHFEAVRAQENE
                     QRLAAYTRQQKEIEEHEEFIARFRAQASKAPQVQSRIKMLEKMEIIELPPEAKRVKIR
                     FPQPARCGQRAIALKNASKSYGDVKVYEGLDFELTRGQKMVFVGHNGAGKSTLLKILA
                     GVLPMDSGERLLGPNVQVGYYTQHRTEMLNPHRTVLEEATDTRRINPDLFVRTVLGTF
                     LFQGDAVYKKAGVLSGGEKSRLALVKLLLDPPNLLLLDEPTTHLDMYSVEALVEALKD
                     FEGTICFISHDLYLINSLADHVLHVDKGQVTLYPGNYEYFQRRQQQKLLEREDAPLPV
                     SRPAAKPTAMPVFAKTSAEDARRLRETQKAHEKKRRKLNAEIRDLEGELADLNGQQAS
                     IFIKSDYQELMKLDAAVKAVEKELVAKMEELEKLNEKV"
     gene            3329..3799
                     /locus_tag="P5079_03565"
     CDS             3329..3799
                     /locus_tag="P5079_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY29097.1"
                     /translation="MKPPADPTKGFTLIELMIVVAIVGVLAAIALPKFADLVRKSKEA
                     ATKGTLGSVRSAISIYYANMEGTYPGDDLTCLTVDSKYLREIPAATVPGQHDTSPVVE
                     TNDTLGMAALLMMDNGRWKYWNWTMGAPAGQKTQGDFWIGCTHTETKGTVWSTI"
     gene            complement(3951..5939)
                     /locus_tag="P5079_03570"
     CDS             complement(3951..5939)
                     /locus_tag="P5079_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29098.1"
                     /translation="MALIELEKITRSYFLDGEELQVLRDVTLKIEEGEFVAIMGPSGS
                     GKSTLMQILGLLDRPTGGLYRLMGKDVSRLSDDEGAAFRRETIGFIFQMFNLLPRTSA
                     LQNVALPMVYAGTPDRDARARELLVQVGLADRLEHKPNQLSGGQQQRVAIARSLANHP
                     RVIFADEPTGNLASDQAEEILSQLVHMNRSGITVIMVTHESDIAAHAKRIIRIKDGRV
                     IADDPSPGKAPGGAAGTSSPRTKPPLPIKIKAEPRSDLGTLWAEFKEYALSAMRGMTA
                     NKVRSGLSMLGILIGVAAVITMLALGKGAQKAIESRLASLGSNLLAMRPGSPSQHGVH
                     GGDYSRFTLDDLKAIAKANPHILRVDGNVQGNVQVVYGNQNANTQVTGVTPAYERMRA
                     STPYAGRFFTPTENLNRERVVLLGQTVVSKLFGDEDPIGKFIKVNRINFKVIGVLPIK
                     GGGGFRDEDDILLIPLNTAMNRLLGQKYLRNISIECDGPDSMEAVTEDITRIMRRRHR
                     LPSYKENDFEIRNMADIQAAISSTTKTITLLLGFVAAISLLVGGIGIMNIMLVSVSER
                     TREIGLRKAVGAARRAILWQFLIESGVMSAVGGLTGILLGALTSFTLSKLAGWTAVVT
                     VQSVMVSFVFSAGVGVLFGLWPARKASLLSPIEALRYE"
     gene            complement(5939..6967)
                     /locus_tag="P5079_03575"
     CDS             complement(5939..6967)
                     /locus_tag="P5079_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY29099.1"
                     /translation="MKKRTILIVVIAAILTVSGVAVWRNKKQKAEERRASRTVEAVLG
                     PLEVSVEATGEVVPLNRVEIQPPIAGRIEKLLVDEGDRVKEGEVLAWMSSSDRAAILD
                     AAKAQGAEEVKRWEDTYKPTPVVAPLSGTVILRNVVVGQTVTAGTILYAISDKLIVLT
                     HVDEVDIGRVRTGMPARITLDAFPGRSVDGKVFNILYEGKNVSNVITYDVKVAPDAVP
                     PFFRSQMTANVKLIISQKENAVLLPADAVREAPDGGRYVLAPSAGGGRPEQRPVEVGA
                     ESGADVEIVSGLKPGDVVLVSRGRYKPQSSDTASSPFMPKRPGARATAAPGGGQTRNG
                     GGSSPRGD"
     gene            complement(6964..8316)
                     /locus_tag="P5079_03580"
     CDS             complement(6964..8316)
                     /locus_tag="P5079_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY29100.1"
                     /translation="MGERHRLGRGKTGGTLVRCGIVALLFWGDGRLSAAPDAAASPIP
                     AAAGAAAEDGGPALTWADCVRRSAAQNPDLLAAQKTRDSRRSQYYGNVNGLLPGVSLS
                     NSYSESNNGARSSRWQTRGSAQWDLFNLKSYASVRSASASLAQADAGLRLASAEVRSG
                     LRKAFAQLLFAQEQVSVAQRIRDVRSNNARMVSLKYQSGRESKGNMLQAQAELAEAEA
                     ELAQARRVQRVARQSLNRWMGQDDFSAVSVTGSFDTKALPARAEVETAVSGHPRLAQL
                     EASRNVARASVQNAWGGVWPTLSAGYGRSWTGPDEFPDETTGWNVSGTLSLPLFSGGP
                     TSAFHSVSSARRNLEKSEQDLRAGRQQLRADLETAWSDLAGKIDRVDVQKKFLEAARQ
                     RNDEALVRYSNGLMTFENWEQVVSGLVSSERGALQSRRDAVLAEAAWEQSLGRGLEEP
                     "
     gene            complement(8333..>8615)
                     /locus_tag="P5079_03585"
     CDS             complement(8333..>8615)
                     /locus_tag="P5079_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRY29101.1"
                     /translation="KQKREEMRTELEKETIDEAKVKAINEELKALGARLADHRLAGIL
                     EVRRILTPEQFKKFQELAGPRGKDKGRGGRRGRGPGRDDADMPPPPDEP"
     assembly_gap    8616..8625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8626..8878)
                     /locus_tag="P5079_03590"
     CDS             complement(<8626..8878)
                     /locus_tag="P5079_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29102.1"
                     /translation="MKKKRCVTEAFLVVGVLFLTASFGWARWPGDGEGPGPRGDGKGR
                     MEEHMKRVEKQLNLSPEQQKKLEEHRAAHRDEAKKFFGEV"
     gene            complement(8891..9370)
                     /locus_tag="P5079_03595"
     CDS             complement(8891..9370)
                     /locus_tag="P5079_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29103.1"
                     /translation="MNHDKMKELVFAMHDGELFPADRSVAESHLASCAECRAALAEWR
                     KTAAAMFPAEKPVASEAFVSAVMRRIETEPAAGFWGRLGGFMETLRTAFSLPRLALAG
                     AAVLAVTVFFLSRPMKTASVPMTEAEVEFVSDLMEGPLQTAENGGAALGTTIEEYFL"
     gene            complement(9386..9961)
                     /locus_tag="P5079_03600"
     CDS             complement(9386..9961)
                     /locus_tag="P5079_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY29104.1"
                     /translation="MNEPENNDEQIIRAVLNGRREEFAHLVRRHQEKVRSLCLSLMRN
                     ATEADDAAQEVFVKAFQALGRFQGRSRFSTWLYRVAYRHCLDLLRSRGRRRTDSLEVL
                     LEERGEGAMPVAASSGGAALENREVLEKILSSLPPEYRLILTLREAQGLSYEELAAAT
                     GTTLDSVKARLRRARQALEEKARHFRALSGV"
     gene            complement(10427..10500)
                     /locus_tag="P5079_03605"
     tRNA            complement(10427..10500)
                     /locus_tag="P5079_03605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10464..10466),aa:Pro,seq:tgg)
     gene            complement(10654..11037)
                     /locus_tag="P5079_03610"
     CDS             complement(10654..11037)
                     /locus_tag="P5079_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY29105.1"
                     /translation="MKKVLVIEDNKCSRRLYERFLGLEGYEVRSAADGRTGLELLKSW
                     KPDLLVLDYMLPDMTGEDICRVLRETPELKSVLILMITAHTYKASDEHCMKMGADGYL
                     EKNFKPEQLKVYVEALLRKGKPSEK"
     gene            complement(11295..12932)
                     /locus_tag="P5079_03615"
     CDS             complement(11295..12932)
                     /locus_tag="P5079_03615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY29106.1"
                     /translation="MKRRMPSRPVTAVLTVGALVSAFLASEVLFRVFVPQYAPSPPPA
                     GTIQFLIDAWGPFAKTERRDGTRRLVKASKDGWLFPEGMPLSPRPGVLRVAVIGESSA
                     GLLAGSLRYLLEGGPSADRVEIIDAAIGGASLEQTERRFGEITGYRPDVVVLLFGQNL
                     GIQHPFMSRWRAAVGGMARRSRLLSYAAESFRRAEETEDRPARLAAFEAALKRMVLTC
                     RERGIRLVLCTSPLNALYPPAKPHAQEFDPRFFEAIYDHSSGRKDRALSELRALSVSK
                     PMAYAFFLRGWWNLAAGNSAAAVVDMERAADLDLDPKRVWSDTNPLIRRISRESGAAL
                     LDFDRLLKNASGKALLGWDEFFDYAHLHEGYRHQEAAWFLRSLEDSGLLKDVRMKPIS
                     SPPVRSWGEMKYPVVEMLYFDNPRFFDAFRCQVSWRLATEGDGFLPRLRRLVSMEEVL
                     SAPAHRRARLTVAIAEGCFERGRWSEGIDYLRMARNDHPSGEDPDVAEAFMHWRRGDT
                     KEALRCFRTAREKNPRPDVVYFAERLSEEPGPRGARR"
     gene            complement(13078..13752)
                     /locus_tag="P5079_03620"
     CDS             complement(13078..13752)
                     /locus_tag="P5079_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29107.1"
                     /translation="METIIRIAHSVVALLGKLLGASWSFFTSDLFRQTVGFASDLFIV
                     LAALAVIAAFFYYQWKTPNIRLKINIKERPKDRWPEPPPPNANRYSIAVALRNEGADI
                     AKGMFFYLAFNEFIEVTDVVFFPDGIQNPTPGKAIFLYNHPHDLPNWSSWIEYFGVQI
                     KIKKNPKQESYCIGRVDYQGGGEKRLKHNWIFFNPKEERFFFMPIHDLKKEMTLGIYK
                     SDSHLE"
     gene            complement(13874..15262)
                     /locus_tag="P5079_03625"
     CDS             complement(13874..15262)
                     /locus_tag="P5079_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY29108.1"
                     /translation="MRLSIKAKIFLASAITLFMMVGLIGFVLNAQNTLAELMNAMIQK
                     NMAAMRVAEQIKYNFVLYDNLLFRYLFTNDKTLLVERERAREKVHGAVRLMRNTAPGS
                     TEKELLGEIEEEIARYDQDVARLLDTYSFSTDEQKKGVVQLIKAIEAGEAPPSVRQTQ
                     KQTLALLSAEGRARLTRIYSQSEKLVDISRAKLEEAQGRVQASVDETERTAVFAGAAA
                     ALGALAVAVLLAVSLLTPLKNLLAGVKKVTAGRLDTELPVEGADEIGGLTREFNTMTR
                     HLREKQERLLTETITDSLTGLHNFRYFQVQLKEEISRAARYKSGFALLIVDIDHFKHY
                     NDTNGHQMGNEILKQVAATLKDTLRPEDFIARYGGEEFVVLLPETGRNNAAVAAQRLR
                     EAVEQAEFAGGQTQPLGRVTISLGGAVYPSDAAAAARLVEKADRALYAAKKAGRNRAV
                     WADEASAADAKA"
     gene            15582..>16402
                     /locus_tag="P5079_03630"
     CDS             15582..>16402
                     /locus_tag="P5079_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29109.1"
                     /translation="MAAVFLATTVLSPFAQAQSIPSSLSSSLVTSAAQETNLGVDIPA
                     DAGALVEFQWFGKESPLTLFHIQDAHGLVPAQFNAARILKAFLAAWRAEKNAKEPEPL
                     LVCVEGAVDRVETDWISAYPSEPVRRRVAQSLLSNGEITGEEYAAILENPRLFEIRGV
                     EDPALYAQHTASKRDTDSLRTALAGPFNELETWLESLKRSFYSAALWKVDHLRRDYLN
                     EKISFHDFLNSLRPLSPAGFSWAAYPQMRASFELAAMESRLNRAELAAEREVLLGR"
BASE COUNT         2864 a         5484 c         4898 g         3146 t
ORIGIN      
        1 gggtggggtt gttcgccggc gcctggctgg gcgccttcgt cgccaacaaa acccccggtc
       61 cttacctgcg tctggcgttc ggactgttcg tggtgggggt cgggatctat ctcatcttcg
      121 gcgcggcgcg gcgattgggt tggctataaa ttccatttca gcgctttgaa ccggcctcga
      181 gccgaagtcc ttccaaaaac cgacgccggg agaccccggc tgagttcagc gcggatcccc
      241 gtaccgttca actgaaaacg ttcagtcccc cagacacact cccaaactcc gcgcggtctt
      301 gatggtgtcg cagtcgagcg gcacctgttt catcttggcc accgcctccg ccaacggcac
      361 cagggtcacc gtgggaggat tgagcgccac catgccgccg aactgtccct cctgaatggc
      421 ccgcaccgcc gccgccccga accgcagcgc gatgagcctg tcgaaggtcg tcggcccgcc
      481 gccgcgctga aggtgtccca gcaccaggga acgcgtttcc ttcccggtcc gcttctcgat
      541 ctccgccgcc accgccgcgc agatgccgcc gagacggacc tcccggccga cctccttggc
      601 ccccaccgtg gacatggaac cgcccttggg cacggcccct tccgccacca ccacgatgct
      661 gaactcccgg ccgcgggcct cgcggtcgcg tatcttttcg cagacctttt cgatgtcgaa
      721 gggaatttcc gggatgagga tcacgtcgga cgtcgacgac acccccgcgt tgagcgcgat
      781 ccagccggcg tagcggccca tcacctccac caccatcacg cgccggtgcg actcggcggt
      841 gctgtggagc ttgtcgagcg cgtcggtggc gatgcccacc gcggtgtcga aaccgaacgt
      901 gaccaccgtg gactggaggt cgttgtcgat ggtcttcggc acgcccacca cggggacgcc
      961 caggtcgttg aaccgcttgg cgatgcggag ggacccgtcg ccgccgatgg ccaccaacgc
     1021 gtccaacccg agctttttga agttcgacac cacctcggcc gagcggtcca ccgctttcac
     1081 ggagccgtcc ggctgtttca tgggatattc gaaggggttc cccttgttgg tggtgccgag
     1141 gatggtgccg cccatggcgg tgatgccgcg cacgtcctcc tcggtgagcg gtcgcacctg
     1201 cgagggatcc aaaaggccgt cgtacccgcg tttgatgccc agcacctccc accccagccg
     1261 cgtggccgac agcaccaccg cgcggatcac cgcgttcagc cccggcgcgt ccccgccgcc
     1321 cgtgttcacc gcaatacgtt ttatctccgg cattttttct ctcctttgaa tttccagtct
     1381 aaactttcca cataaagccc gggaccccga tcaaaccttc tcgttcagct tttccaattc
     1441 ttccatcttc gccaccagct ccttctccac ggccttgacg gcggcatcca gcttcatcag
     1501 ttcctggtaa tcgctcttga tgaagatcga ggcctgctgg ccgttgaggt cggccagctc
     1561 cccctccaga tcgcgtatct cggcgttgag tttgcggcgc tttttttcat gcgccttctg
     1621 cgtttccctc agacgccggg cgtcttccgc cgaggtcttg gcgaacaccg gcatggccgt
     1681 gggtttggcc gccggccggg aaacggggag cggcgcgtcc tcccgctcca gcaatttctg
     1741 ctgctgccga cgctggaaat attcgtagtt gccgggatac aaggtcacct ggcccttgtc
     1801 cacgtgcagc acgtggtcgg cgagggaatt gatgaggtag aggtcgtggc tgatgaaaca
     1861 gatggtccct tcgaaatcct tcagcgcctc caccaacgcc tccacgctgt acatgtcgag
     1921 atgggtggtg ggttcgtcga gcaggaggag gttgggcggg tcgagcagca gcttcaccag
     1981 ggccagccgg ctcttctcgc cgccgctcag caccccggcc ttcttataca ccgcgtcgcc
     2041 ctggaacagg aacgtgccca gcacggtgcg cacgaacagg tccgggttga tgcgccgggt
     2101 gtcggtggcc tcctccagca cggtgcggtg ggggttgagc atctcggtgc ggtgctgggt
     2161 gtaatagccc acctgcacgt tggggcccag cagacgttcc ccgctgtcca tcggcagcac
     2221 gcccgcgagg attttcagca gggtggactt gccggcgccg ttgtgtccca cgaagaccat
     2281 cttctggccg cgcgtcagtt cgaagtccag tccctcgtag accttcacgt cgccgtagga
     2341 tttcgacgcg ttcttcaggg cgatggcccg ctgcccgcag cgggcgggct gggggaaccg
     2401 gatcttgacc cttttcgcct ccggcgggag ttcgatgatt tccatcttct ccagcatttt
     2461 gatgcggctc tgcacctgcg gcgccttgga ggcctgggcg cggaaccggg cgatgaactc
     2521 ctcgtgctcc tcgatctcct tctgctggcg ggtgtaggcg gcaagccgct gttcgttctc
     2581 ctgcgcccgc accgcctcga aatggtcgaa atccccggga taacggaaaa gtttatggtc
     2641 cctcaactcc accacggcgt gcaccagcgc gttgacgaag gcccggtcgt gggagatgag
     2701 caggaggccg catttggctt tccgcaggta ttcctggaac cacaacagag actccaggtc
     2761 cagatggttg gtgggttcgt cgagcaggag gacgtcgggt ttctgaacca agagccgggc
     2821 gatggccacg cgcatcatcc agccgccgct cagctgcgcc accggacggg aaaaatcttc
     2881 cagccggaac cccagcccca tgaggacctt cttcgcctcg gccgagcgac gctcgtccac
     2941 cgacgccgac tcttcccggt cggaggacag cgcctcctcc agcaccgtgc cgccgccgaa
     3001 cggcgccgtt tcctgcggca gatgccccag ccgcaacccg cggcggcgga tgatctcccc
     3061 gccttccacc ggggtttctc ccaggatcat ccggaacagc gtggatttcc ccgccccgtt
     3121 ggggcccacc accgccaggc ggtcgctctc gttcatctgc agagaggccc cgtcgaaaac
     3181 cacctgagcc ccgtaggcct tatggatccc cttgaccgtc aacaccgtcc gttcccgccc
     3241 cccgggccgc gccccattaa tttcaaaagc aaaaccgatt gaccgcccat atcatatata
     3301 atgtcagcaa gatgcccggt ccgaaaacat gaaacccccc gccgatccga caaaagggtt
     3361 caccctcatc gaactgatga tcgtcgtggc catcgtcggc gtcctggcgg ccatcgccct
     3421 gcccaaattc gccgacctgg tccggaaatc aaaggaagcc gccaccaagg ggacgctcgg
     3481 gtccgtccgt tccgccattt ccatctacta cgccaacatg gaagggacct accccgggga
     3541 cgacctcacc tgcctgaccg tcgacagcaa gtacctgcgc gagatcccgg cggccacggt
     3601 gccgggccag cacgacacct cccccgtggt cgaaaccaac gacaccctcg gcatggccgc
     3661 cctcctcatg atggacaacg gccgttggaa atattggaac tggacgatgg gcgcgccggc
     3721 cggacaaaaa acacagggag atttctggat aggctgcacg cacaccgaaa cgaaagggac
     3781 cgtctggtcc accatctaag caccaccggc cttttttagc cgttcctccc tccgcctctt
     3841 caaacgccgc cgtctccccg aaaaaaaacg cccgcatgcc gtcgttgcac aggaaccgat
     3901 tgtagcaaag atttttccgc cgccggaaag cggcggggat cggtggggtt ttattcgtac
     3961 cgcagggctt cgatgggcga aagcagcgag gctttgcgag cgggccaaag gccgaacaga
     4021 acgccgaccc cggcggaaaa tacgaacgac accatcacgg actgcaccgt gaccaccgcg
     4081 gtccagccgg ccagtttcga caacgtaaag gaggtcagcg cccccaaaag gatgccggtc
     4141 agtccgccga ccgccgacat cacgcccgac tcgatgagga actgccacag gatggcccgc
     4201 cgcgccgcgc ccaccgcttt gcgcagaccg atctcgcggg tgcgttcgct caccgacacc
     4261 agcatgatgt tcatgatgcc gatgccgccg accaggagcg aaatggccgc cacgaacccg
     4321 aggagcagcg tgatggtctt ggtggtggaa ctgatggccg cctggatgtc ggccatgttg
     4381 cgaatctcga aatcgttctc cttataggaa ggcaggcggt gacggcgccg catgatccgc
     4441 gtgatgtctt cggtcaccgc ttccatgctg tcggggccgt cgcactcgat ggagatgttc
     4501 cgcagatatt tttggcccag caggcggttc atggccgtgt tgagcggaat gaggaggatg
     4561 tcgtcctcgt cgcggaaccc gccgccgccc ttgatgggca acacgccgat gaccttgaag
     4621 ttgatgcggt tcaccttgat gaacttgccg atggggtcct cgtccccaaa aagtttgctc
     4681 acgacggtct gccccagcag gaccacccgc tcgcggttga ggttttccgt cggcgtgaag
     4741 aacctcccgg cgtagggggt ggacgcccgc atgcgttcgt aagccggcgt gacgcccgtc
     4801 acctgcgtgt tggcgttctg gttgccgtag accacctgca cgttgccctg gacgttgccg
     4861 tcgacccgca ggatgtgcgg gttggctttg gcgatggcct tgaggtcgtc gagcgtgaac
     4921 cggctgtaat cgccgccgtg cacgccgtgc tgggacggcg accccggccg cattgccaac
     4981 aggttggagc ccaggctcgc caggcgcgac tcgatggctt tttgcgcgcc tttgcccagg
     5041 gccagcatgg tgatgaccgc cgccacgccg atgaggatgc ccagcatgga gaggccgctg
     5101 cgcactttat tggccgtcat gccgcgcatg gccgagaggg cgtattcctt gaactccgcc
     5161 cacaatgtgc ccagatcgga ccgcggttcc gctttgattt ttatcggcaa agggggcttc
     5221 gtcctcgggg acgatgtccc cgcggcgccg ccgggcgctt tccccggtga ggggtcgtcg
     5281 gcgatgaccc ggccgtcttt gatgcggatg atgcgtttgg cgtgggcggc gatgtccgac
     5341 tcgtgggtga ccatgatgac ggtgatgccc gaccggttca tgtgcaccag ctgggagagg
     5401 atctcttccg cctggtcgga cgccaggttg cccgtgggtt cgtcggcgaa gatcacgcgg
     5461 gggtggttcg ccagggaccg cgcgatggcc acacgctgtt gttgcccgcc ggaaagctgg
     5521 ttgggtttgt gttcgagcct gtccgccaaa cccacctgca ccaacagctc cctcgcgcgc
     5581 gcgtcgcggt ccggcgtgcc ggcgtacacc atgggcaggg ccacgttctg cagggccgac
     5641 gtccgcggca ggaggttgaa catctggaat atgaagccga tggtttctcg acggaaggcg
     5701 gcgccttcgt cgtcggacag gcgggagacg tcctttccca tcaaccgata caggccgccg
     5761 gtgggacggt ccaacaggcc caggatctgc atgagcgtgg acttgccgga accggacggg
     5821 cccatgatgg cgacgaactc gccctcctcg atcttgagcg tgacgtcgcg gaggacttgg
     5881 agttcctccc cgtccaaaaa ataggaccgc gtgatttttt ccagctcgat gagggccatc
     5941 agtcgcctcg gggggaggaa cctccgccgt tgcgggtttg gccgccgccg ggggcggccg
     6001 tggcgcgcgc gcccggccgt ttgggcatga aggggctgga ggcggtatcg gaggactgcg
     6061 gcttgtaacg gccgcgggac accaggacga cgtcgcccgg tttcagtccg gacacgatct
     6121 ccacgtcggc gccggattcc gcgcccactt ccacggggcg ctgttccggc cggccgcccc
     6181 cggccgacgg cgccagcaca tatcggccgc cgtccggcgc ctcgcgcacg gcgtcggcgg
     6241 gcaggagcac cgcgttctcc ttttggctga tgatcagctt cacgttggcc gtcatctgcg
     6301 aacggaagaa cggcggaaca gcgtccggcg ccaccttcac gtcgtaggtg atcacgttgg
     6361 acacgttctt gccctcatac aagatgttga acacttttcc gtccacggaa cggccgggga
     6421 aggcgtccag ggtgatgcgc gccggcatgc ccgtgcgcac gcggccgatg tccacctcgt
     6481 ccacgtgcgt gagcacgatg agcttgtcgg aaatggcgta gaggatcgtg cccgcggtca
     6541 cggtctggcc caccaccacg ttgcgcagga tgaccgttcc ggacagaggc gccaccacgg
     6601 gcgtgggctt gtaggtgtct tcccatcgtt tgacctcctc cgctccctgg gccttggcgg
     6661 cgtccaggat ggccgcccgg tcggacgaac tcatccacgc caacacctcc ccctccttca
     6721 cgcggtcgcc ttcgtccacc aaaagttttt cgatccgccc cgcgatgggc ggctggattt
     6781 ccacgcggtt gagcggcacc acctcgccgg tggcctccac gctgacctcc aggggcccca
     6841 ggacggcctc caccgtgcgg gaggcccggc gctcctcggc cttttgtttt ttgttccgcc
     6901 aaacggcgac ccccgatacc gtcaggatcg ccgcgatgac gactattaaa atggtgcgtt
     6961 tcttcatggt tcctccagtc ctcgtcccaa agattgttcc caagcggctt ccgccaggac
     7021 ggcgtcccgc cgcgactgca acgcgccgcg ttccgaactc accaaaccgc tcacgacctg
     7081 ttcccagttt tcaaacgtca tcaggccgtt ggaataacgc accagcgcct cgtcgttcct
     7141 ttgtcgcgcg gcttccagaa atttcttttg cacgtccacc cggtcgatct ttccggccag
     7201 gtcgctccag gccgtttcca ggtcggcgcg gagctgctgt cggccggcgc ggaggtcctg
     7261 ttccgacttt tccaggttgc gccgcgccga cgacacggaa tggaacgcgc tggtgggccc
     7321 gccagaaaaa agaggaagag acaacgtgcc ggagacgttc cacccggtgg tctcgtccgg
     7381 gaactcgtcg ggccccgtcc aggaacggcc gtacccggcg ctgagcgtgg gccacacgcc
     7441 gccccaggcg ttctgcaccg acgcccgcgc cacgttacgc gacgcctcca gctgcgccaa
     7501 ccggggatgc ccggaaacgg ctgtttccac ctccgcgcgc gcgggcaggg ccttggtgtc
     7561 gaaactcccg gtgacggaaa ccgcggagaa atcgtcctgc cccatccacc ggttcaacga
     7621 ttgccgggcc acgcgctgaa cgcgccgcgc ctgggccagc tcggcctcgg cctccgccag
     7681 ctccgcctgg gcctgcagca tgttcccttt ggactcccgc ccggactggt atttcaagct
     7741 caccatgcga gcgttgttgg agcgaacgtc gcggatgcgc tgcgccacgg acacctgctc
     7801 ctgcgcgaag agaagctggg cgaaggcctt ccgcaacccg gaccgcacct cggcggaagc
     7861 caaccgcaga cccgcatccg cctgcgccag ggacgccgag gccgaccgga ccgaggcgta
     7921 gctcttcagg ttgaaaaggt cccactgcgc cgaaccgcgc gtctgccacc gcgaggaccg
     7981 cgcgccattg ttcgactcgg aataggaatt ggagagggac acgccgggca aaagaccgtt
     8041 tacgtttccg taatattggg accggcggga atcacgcgtc ttttgcgccg ccagcagatc
     8101 cgggttctgc gccgccgacc ggcgcacgca atcggcccac gtcagagccg gaccaccgtc
     8161 ctccgccgcc gcccccgccg cggcggggat cggagacgcg gcggcgtccg gcgcggccga
     8221 gagccgcccg tccccccaaa agagaagggc gacaatcccg caccggacga gtgttccgcc
     8281 ggtcttcccg cgacctaatc gatgccgttc gcccacaaaa cgccttccct tctcaaggtt
     8341 cgtccggcgg cggtggcatg tccgcatcgt cacgccccgg accgcgccct cgacggccgc
     8401 cgcggccctt gtctttccct cggggccccg cgagttcttg gaattttttg aactgttccg
     8461 gcgtgaggat gcgccgcacc tccaggatgc cggccaatcg atggtcggcc agtcgggcgc
     8521 cgagggcctt caactcctcg ttgatcgcct tcaccttcgc ctcatcgatc gtttcctttt
     8581 cgagttccgt ccgcatctcc tcccgtttct gtttcnnnnn nnnnntcact tccccgaaaa
     8641 acttcttcgc ctcgtcccga tgggcggcgc ggtgttcctc cagtttcttc tgctgttcgg
     8701 gcgacagatt caactgtttc tccacccgtt tcatgtgttc ttccatgcgg cctttcccgt
     8761 cgccgcgcgg acccggcccc tccccatcgc ccggccaacg ggcccagccg aaggacgccg
     8821 tcaggaacag aacaccgacg accagaaacg cttccgtcac acaccttttt ttcttcataa
     8881 cgcctcctcc ttaaaggaaa tattcctcaa tggtggtgcc gagcgccgcg ccgccgttct
     8941 cggcggtctg gagcggcccc tccatcaggt ccgacacgaa ctccacctcg gcctcggtca
     9001 tggggaccga agcggttttc atggggcggg acaaaaagaa aaccgtcacg gccagaacgg
     9061 ccgcgccggc cagcgccagg cggggcaggg aaaacgccgt ccgcaacgtt tccatgaaac
     9121 cgccgagccg cccccagaaa ccggccgccg gttcggtctc gatgcgccgc atcacggcgc
     9181 tgacgaacgc ctccgacgcc accggctttt cggcagggaa catcgcggcg gcggttttcc
     9241 gccattcggc cagggccgcc cggcattccg cgcaggaagc caaatggctt tccgcgacgg
     9301 accgatccgc cggaaacagt tccccgtcgt gcatcgcaaa aaccagttct ttcatcttat
     9361 cgtggttcat gttcgtcctc tccgtttaga cgcctgaaag agccctgaag tgtcgcgctt
     9421 tttcctcaag cgcctgccgc gcccgcctca gccgggcctt cacggaatcc agggtcgttc
     9481 ccgtggccgc ggccagttcc tcataggaca gcccctgcgc ctcccgcaac gtcaagatga
     9541 gccggtactc cggcggcaga gaggacagga ttttctccag gacttcccgg ttctccaacg
     9601 ccgcaccgcc ggaagacgcc gcgaccggca tcgcgccttc cccccgttcc tccaaaagga
     9661 cctccagcga atccgtccga cggcgccccc gggaccgcaa caggtccaga caatgccggt
     9721 aggccacgcg gtaaagccag gtggagaaac gggaccggcc ctgaaaacgg ccgagggcct
     9781 gaaacgcctt gacgaagact tcctgggcgg cgtcgtcggc ttcggtggcg ttgcgcatga
     9841 gggaaaggca aagcgaacgc actttttcct ggtggcgccg caccagatgg gcgaactcct
     9901 cccggcggcc gttcaacacg gcccggatga tttgttcgtc gttattctcg ggttcgttca
     9961 tggggtcttt gccttctctt gagacgcgcc gccaatgaaa aaagtgtcgc gccggccggc
    10021 gcgccgccac aaatatagct tatccggcgg cggcgggcga tgagcggcag gcaaaagata
    10081 ccccgaacga tttcaaaacg ctcgattgtc ggaaattttt aatgggagga acaaacagcg
    10141 gctattcgga gaggactatt gtccgtttca tgtttgggtt acttaaaaac gtaaaagcgg
    10201 aacatcttac atcttgctct gtcattgaga gtcgcgtctt ttcgacgaag caattccttg
    10261 tttcccgaaa gaggggatcg ccacaacagc ggccccacgc aggccagccc gttttgctcg
    10321 caggaggccg cactctaaat ggtcggggcg ccctcattca gaaacttagg cgaattaaaa
    10381 atcaattcac ctaatgaacg aggacagccc ttgtgaccca atagtgtcgg ggcgactgga
    10441 cttgaaccag cgacctcctg ctcccaaagc aggcgctcta gccaactgag ctacgccccg
    10501 gcggggtcgt cggccctatg ggccgccgcc ggcaccttaa gccctgcgtg gtgccggttc
    10561 ccaaaaattt tcgcttgggc tgtcctcgcg cgtgagccgg attttgttca tccggcgatg
    10621 cttttgcgtg agggcgcccc gtaacaaccg gacttatttt tcggacggct tgcccttccg
    10681 caacagggcc tcgacgtaaa ccttgagctg ttccggcttg aaattttttt ccaggtaccc
    10741 gtccgccccc atcttcatgc agtgctcgtc cgacgccttg taagtatgcg cggtgatcat
    10801 gaggatcaac accgatttta attccggcgt ctcgcggagg acgcggcaga tgtcctctcc
    10861 ggtcatgtcc ggcagcatgt aatcgagcac caggagatcg ggcttccagc ttttaagcaa
    10921 ttccaggccg gtccggccgt ccgccgccga acggacttca tagccttcca gtcccagaaa
    10981 acgctcgtag agccgccgcg aacatttgtt gtcctcgatg accaatacct ttttcatcgt
    11041 ttttctcccc cccaggttac ccgccgaatc gccgccactc gataagcccc aaggccggcg
    11101 gaaccggccc ccccttcagc cttccgatta tactaaacgt caccggctat tcttcctgtt
    11161 tgagtttccg ttttgccgcc ggacggaaat accgcttccg gtctcggtga aatttcgtcg
    11221 gcggagagat tttccttcgc ccctttaatt tcgggacttt gaactttatc ggcataaaac
    11281 ttcccccgaa accgtcaccg gcgcgcaccg cgcggcccgg gttcctccga aaggcgctcc
    11341 gcgaagtaga ccacgtccgg ccgcggattt ttctcccggg cggtcctgaa acaccggagc
    11401 gcctccttcg tgtccccgcg ccgccaatgc atgaaagcct cggccacgtc cggatcctcc
    11461 cccgacggat gatcgttccg cgccatccgg agataatcga ttccttcgga ccagcgcccc
    11521 cgttcgaaac atccctccgc gatcgccacg gtcagccgcg cccgtcgatg ggccggcgcg
    11581 gacagcacct cttccatcga gacaagccga cgcagccgcg gcaggaatcc gtctccctcg
    11641 gtcgccagac gccaggaaac ctggcaccgg aaagcgtcga agaaccgggg attgtcaaaa
    11701 tacaacattt ccacgacggg atacttcatt tccccccagg accgtaccgg cggagaagaa
    11761 atcggtttca tccgcacgtc cttcaagaga ccggaatcct ccaacgaacg aaggaaccag
    11821 gcggcctcct ggtggcgata tccctcatgc agatgcgcgt aatcgaaaaa ctcgtcccaa
    11881 cccaacagcg ctttccccga tgcgtttttc aggagccgat cgaaatccag gagcgccgct
    11941 ccggattccc ggcttatccg ccggatcaga ggattcgtgt ccgaccacac ccgcttcggg
    12001 tccaaatcca ggtccgcggc cctttccatg tcaacgacgg cggcggccga gttccccgcc
    12061 gccaggttcc accatccccg caggaaaaag gcatacgcca tgggcttgga aacggaaagg
    12121 gcccgcaact cggacaaagc cctgtccttt cggcccgagc tgtgatcata gatcgcttcg
    12181 aagaaacggg gatcgaactc ctgcgcgtgg ggctttgccg gcggataaag cgcgttcaga
    12241 gggctggtgc acaggacgag gcggatgccc cgctcacggc acgtgaggac catccgtttg
    12301 agggcggctt cgaaagcggc gaggcgcgcg ggacggtcct ccgtctcctc ggcgcgacga
    12361 aacgattccg cggcgtaaga aaggaggcgt gaacgccgcg ccattccccc cacagccgcg
    12421 cgccagcggc tcatgaaggg atgttgtatc cccaggttct gtccgaacag aaggacgacc
    12481 acgtccggcc ggtagccggt tatctcgccg aaccgccgtt ccgtctgttc gaggctggcc
    12541 ccgccgatgg ccgcgtcgat aatctccacc cggtcggccg aagggcctcc ctccaaaaga
    12601 tatcggaggg acccggccag cagcccggcg ctcgattcgc cgatcaccgc aacgcgaagg
    12661 acgcccggcc gcggagacaa cggcatccct tcggggaaga gccagccgtc cttcgacgcc
    12721 ttcaccagtc gtcgcgtgcc gtcgcgccgc tccgttttgg cgaacggtcc ccaggcatct
    12781 atcaggaatt ggatcgtgcc ggcgggcgga ggggaggggg cgtattgggg gacgaagacg
    12841 cggaacaaca cttccgaggc caggaacgcc gaaacgagcg ctcccaccgt caggacggcg
    12901 gtcaccgggc gggaaggcat ccgtcttttc atggggaggc gtttcctccg tccgaagccc
    12961 ccgcgccgaa gagcgcatcc catcggcgcg gcatcgtcac cgaacttttt ccgtcggggc
    13021 aggggaccct cgctccgccg acggtcggaa acgtccgcgg gcggcgggcc ccggaaatca
    13081 ttccagatgg ctgtcggatt tgtagatgcc cagggtcatt tccttcttca ggtcatggat
    13141 gggcatgaaa aagaagcgtt cttcttttgg gttgaaaaat atccagttgt gcttgagccg
    13201 tttttcgccg ccgccctgat agtccacccg gccgatgcag taggattcct gtttggggtt
    13261 cttcttgatc ttgatctgca cgccgaaata ttcgatccac gaggaccagt tgggcaggtc
    13321 gtgcgggtgg ttgtagagga agatggcctt gcccggggtg gggttctgga tgccgtccgg
    13381 aaaaaaaacc acgtcggtga cctcgatgaa ctcgttgaac gccaggtaaa agaacatgcc
    13441 tttggcgatg tccgcgccct cgttgcgcag ggccacggcg atggaatagc ggttggcgtt
    13501 gggcgggggc ggctcgggcc agcggtcctt gggccgttcc ttgatgttga tcttgaggcg
    13561 gatgttgggc gtcttccact gataatagaa gaacgcggcg atgacggcca gcgccgccag
    13621 cacgatgaag aggtcggagg cgaaaccgac ggtctgccgg aaaaggtcgc tggtgaagaa
    13681 actccacgag gcccccagca acttccccag aagcgcgacg acggaatggg cgatgcggat
    13741 gatggtttcc atgtctttcc ctgtcctttc ggtttcagcc cggcggtttc aagcgacgcc
    13801 gctgtcgatt ctataaaaaa atccctaaaa acaccggccg gcggaacgat tcccggaaaa
    13861 aatcctccga acgtcaggcc ttggcgtcgg cggcggacgc ctcgtccgcc cacacggcgc
    13921 ggttgcggcc ggcttttttc gccgcgtaaa gcgcgcggtc ggccttttcc accagccggg
    13981 ccgccgccgc ggcgtccgac ggatacaccg ccccgccgag actgatggtc acccggccga
    14041 ggggctgggt ctgtccgccg gcgaactcgg cctgttccac cgcctcgcgc aggcgctggg
    14101 ccgccaccgc ggcgttgttg cggccggtct ccggcaggag gaccacgaac tcctcgccgc
    14161 cgtaccgggc gatgaaatcc tcgggacgga gagtgtcctt cagggtggcc gccacctgtt
    14221 tcaaaatctc gttgcccatc tgatggccgt tggtgtcgtt gtagtgtttg aagtggtcga
    14281 tgtcgacgat gagcaacgcg aaaccgctct tatatcgcgc ggcgcgggag atctcctcct
    14341 tgagctgcac ctggaaatag cggaagttgt gcagccccgt gagggaatcg gtgatggtct
    14401 cggtcaggag gcgttcctgt ttttcccgaa ggtggcgcgt catggtgttg aattcgcggg
    14461 tgagtccgcc gatctcgtcg gccccttcca cgggcagttc cgtgtccagc cggccggccg
    14521 tcactttctt cacgccggcg aggaggttct tcagcggcgt caagaggctc acggcgagga
    14581 ggaccgccac cgccagcgcg cccagggcgg ccgccgcgcc cgcgaaaacg gcggtgcgtt
    14641 cggtctcatc cacggacgcc tgcacccgcc cctgggcctc ctccaacttg gcgcgggaga
    14701 tgtccaccag tttttcggac tgcgaataga tgcgcgtgag gcgcgcccgg ccttcggcgg
    14761 aaagcagggc cagcgtctgt ttctgcgtct ggcgcaccga cggcggcgcc tcgcccgcct
    14821 cgatggcctt gatgagctgc accacgccct ttttctgttc gtcggtgctg aaggaatagg
    14881 tgtccaacag gcgcgccacg tcctggtcgt agcgcgcgat ctcctcctcg atctccccca
    14941 aaagctcttt ttccgtcgat cccggggcgg tgttgcgcat caggcggacg gcgccgtgca
    15001 ccttttcgcg ggcgcgttcg cgttccacca acagcgtttt gtcgttggtg aagaggtagc
    15061 ggaagaggag gttgtcgtaa aggacgaagt tgtatttgat ctgctccgcc acgcgcatgg
    15121 cggccatgtt cttctggatc atggcgttca tcagttcggc cagggtgttc tgcgcgttca
    15181 agacgaaacc gatgagcccc accatcatga acagcgtgat ggcggaggcg agaaatatct
    15241 tggctttgat ggaaagacgc atcgtcaaag gtcctcgaca gggattatac cacgcgcttc
    15301 ccgttcttcg caccgaaaac cgtgacggca tcctgttttt ttaccgatcc cgggcgacac
    15361 cgcccctccc acccgcccct tccgatttgt tacatatttg ttacacatac ttccccctcc
    15421 tttaaccccc ctccgctaga attcttttat cacccctggc acctttcacc ttcttcactc
    15481 atatttagaa aattgtaact agatttgtaa cttgtgggac atccatgggc ttaaaacgta
    15541 aatctatccg acgaattctt ctcaaaacga ccgcctcgag cgtggcggcc gtttttcttg
    15601 ccaccaccgt cctttccccc ttcgcccagg cccagtccat cccctcttct ttatcgtctt
    15661 ctcttgttac gtcggccgcg caggaaacga atctgggggt ggacatcccg gcggacgccg
    15721 gcgccctggt ggagttccag tggttcggga aggaatcccc gctgacgctg ttccacatcc
    15781 aggacgcgca cggcctggtg ccggcccagt tcaacgccgc ccggatcctc aaagcttttc
    15841 tggcggcctg gcgcgccgaa aaaaacgcaa aagaacccga accgttgctc gtctgcgtgg
    15901 aaggcgccgt cgaccgggtg gagaccgatt ggatctccgc ctatccctcg gaacccgtgc
    15961 ggcggcgggt ggctcaaagc ctgctctcca acggcgagat caccggcgaa gaatacgccg
    16021 ccatcctcga gaatccccgc ctgttcgaga tccgcggcgt ggaagatccc gccctctacg
    16081 cccagcacac cgcctccaaa cgggacacgg attccctgcg caccgccctg gccggcccgt
    16141 tcaacgagtt ggagacctgg ctggaatcgc tgaaacggtc cttttattcc gccgccctgt
    16201 ggaaagtgga ccacctgcgg cgggactacc tgaacgaaaa aatctccttc catgactttt
    16261 tgaattccct ccggccgctc tcgcccgcgg gtttttcctg ggcggcctat ccgcagatgc
    16321 gggcctcgtt cgagctggcg gccatggagt cccggttgaa ccgcgcggaa ctggcggcgg
    16381 aacgcgaggt gttgctgggc cg
//
LOCUS       DAOTFD010000033        15992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2484_length_15992_cov_4.136977, whole genome shotgun sequence.
ACCESSION   DAOTFD010000033 DAOTFD010000000
VERSION     DAOTFD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2484_length_15992_cov_4.136977"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..12463
                     /locus_tag="P5079_03635"
     CDS             <1..12463
                     /locus_tag="P5079_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRY29110.1"
                     /translation="SERSGEVVFNTAITGYQENLQDWVTGQRESIQESWDRLEAYRQE
                     ILSGGSAGGNTITPGEQKQLANLEYQWNKKVSEFQQALPLREQELATRIAKANDSLQH
                     NQEMLNIGREMASLLPVKAEVSGVIGLLDKLFNHKLYSNAARLRMAQDVGNGVDSMFL
                     SMEKTLASFARENIDALRTGSQVDLIFSGANTPQQFAAGMGQLTDELVRIMETMKRQA
                     LFNAILTKGREPTVQELEAISRDFAEYEKELKSAYLEGFGQASAQNVDSLLLRYLGRV
                     QTVWNEALQQAGKSPKIAQEVDLQQIFKQTLLRDPKQGPVGMEDYKLGLQRAVMAATS
                     AISDPLQMTPPGAPLVQELETVRPDMLAESVDAQNFFQKDVQMFHWYMMRYIASVRPN
                     YKMDPALENFWKDAASLSLKDKQARLEQYAQLLDRLLQDINPSYSKGTLTTLLYNSLR
                     SSSDKVGVWQPEDVIKLGFLPLMQFMDRQLTNLNNLNMLEGKPFPALAEDVITGSYVR
                     DAAAAPRAFTDGMVASLRLLNQIYGMDTNTGTAELQAKLQEIFGAPRDVNDLMPRLEN
                     AVRQMDELLGQRIRSQGGYYESGTLVRSFNDAYRGVPQVDLGTARGVYNRVMDDLQDI
                     LSKAYTVRKDNLTVYGSIPDGVTLEQFGSEEHLGKFLQGMLLDLTRLEQIALYNHPEN
                     RALIEQTFNGIQRDIYAGYRSSFIDSKPDDMIDLIGRVSNLLRSYEGGLRQAVQWVDK
                     DHAVRHGLLDDAFMEKALGEQGQRDGAGEPTLNDLKKGLQHADDAADSLIQQVSTKIT
                     REHDVLLSGQTRRDFFANDFFADPQVIEALRTDVARSHKVLLQGIRLVYQTDDTKALI
                     DPLQNAPDVSGLRNATLDQAKQWVVDYARRADDVLQTVGVRYSAGMLPKQIEESMMSA
                     RSPGEAVQIGMSRMNALLSEHLKSVFTWKNQWRILPDIAEETTETMRSVTSVSQVADA
                     VRQDVERFVEAYKLLSGDQSLNAEEIIGTPYQGQDPAALTPWMDGLVRRLENFLNAHP
                     IPGMYRQKGILLEAAATARRGASLGNPLAVEKYVYTEVMAKAQSVLEQTFFTRAQTIG
                     AAIHAPLPENYRMSSNIDLAIADYDIHDNDIIIILSDRTRVRLSGIPTPANKEASLAL
                     ENAIRDVLRRGTNVVLVTERNERGLSVTGHLYLPAETTAAARYPGLYMDANAVLQGRG
                     FGHRSPDEILPLSLYTTDNMVSEEQLLTNMEATAKNLADMAYFLSKGDNRLREAILAL
                     AQKQFGRARPGTDLTPDSMGGKIQQVITRPLRHQPVEEVVSQGRVNERVEAFFREVLP
                     LLREASNRAGGHLLEADQKDLDYNMMTLFESLRGSHGDMQSIALSYRWGMEFLSKMMV
                     KPMDMSVPDLDRDFNAAMAGVQDPADFAYQQGEISVKLEKLFRAVAERHGFKISDESF
                     QAINEASKKFQFLGSEGGLTPQVRAYLEAFDQVLRQELGKMGYAYRGNLARDVFDPAN
                     IARLGRGPLDHVSSLWGKRSATPDLTTLRGAMLAYENGVNRIKNILADANSDLTFRAL
                     SGQSVSPLDDELSLELRKLEALPYQQKAALVALGIAPETIFTRRDRIYKFFRDTVLRD
                     ILIYSVGRLIIRLTFGLLFGFVWYVILLTPALIYKTLIFMAAHEWGPFKGRQFEPVKG
                     LAGVKEFLGMPSVFSPYKYSVVKMFSAKSMETQKVQTGLKIHESVYRFSPLSQKWLLF
                     CARFAGGLDSLPKRNIVLWILSQATVRPIVWVIQLLELDQALLGFGLMWTEALSSIGR
                     LFGGPTAKTDAAKRPSRIKHGLYRLLIPVLAVFISVAGPFFMSKYRPASPLETLSSIV
                     KSEQVLVQKFTAPAPAPVQVERPWTAPPASETASQPAPEAAPTQNVLSSDEALLQGIY
                     APAGVFPLDVRAVTTGHDYSFTSLPDGQAVLNDDFGVPHTKHPGSQHDGVDVFANTGT
                     PVRSVVAKGVIETVNRPVGTDRGTVVSVRDDEGNLYVYYHLGASLPAGVAVGAKVENL
                     AGGVIGYVGTTEDAMNTHPHLHFQIIPRGQSKAVSPFPSLSHWSQQLDLTTPTAPSAF
                     GNDFSWLPFVNLLTGLQDASDDVIDAVQGVLREYRLLESDTEPDQYLQLLKQLPPHAH
                     LPYVLARWILEKAQFSLTPAAADTRHAPLVRRAVDLTRQVWPEYQPDDFVFVAGGMGK
                     SLGKRLFARALDTRTGRTKILVHEDVLQDLEARARKLAETSGAQLTQVLKLLLVGLLH
                     DLAESSGVSHDEIETAGMSLTRNDSLAYENFDAILRAGEDLRREGTGTPAEETPAVLP
                     PARAMAPVKIVSAVLYTAAISGLAAAFSALFTLNLPALVLFGLPALLVAVPFTVQAAQ
                     YLRLLTHPMNKTYFETGRRLRFKPSDEAVIREEWRDIIKRLWNTDDLAALYARFLEEK
                     KQLDTDLAALHETARGLQGLLAVNPRHPVHRQEALHVRQEILRKENESRVLRARLELS
                     GLDKFIMESLPPETSPLVAPADKLAYWTVIDISNFQRAVGSLQEVSFRVLNRSNTGFR
                     WSLAYGLGLLFNGWMGKVSQRKAPPLAQTRWVTRNGRLVPYIELTPALLLNRTALRQA
                     LAHEWGESRNLGEATSLKLENRLVPAAWTHTLFNGPLAWLDEKVTRLLGWEPGRVEQI
                     LGQLVMIGFFLWVGSLLAVKSGAAVLMGATIKTTDNVDFYEPETARTRSQDEVLGLLS
                     SHAAILTKIINHLVKTGTHVVGIQDSRWENGKLHLFISSRNAQEFSSVLKRGESFALS
                     NDPRAPKYRIEKFDHSTGRLTAVPANDNAPSRGPPFVNTIYFIPNTASENIQRERLEN
                     IAREIRTNGLTHSPLINHVLGLAKPAKPMTLRKVENSLKAVHFFDKNVENDPSQRIVV
                     GLSLRMLQHPEQKLLLVRGPPGTGKTTTAQEIIRQATAQGLRVLLVSQMNQAVDNALQ
                     AFVDSDFPALRASNDPPETMREYGTSRLWMGNPSEDSGTALFLQRFAKTEKGGFVVGA
                     TNVGAVTTHVNQKLAQLLNKGTFDLVIMDEASRDNLAGSLLPLTLAKKAIIIGDEHQL
                     PTYPMAGMNAGQASQELDVDAPHYRDFNMSLFERLLASRQATVAMPSVNRRSHPLIAK
                     FISVIFYAGRLETKPWETARPDTLVIKDTSRMGMWEEKDANNSVFNVGEAGLIVDAYG
                     EHLRAGNSADNVTIISPYMAQIALISRMIRQRGLRVPETTTVDAFQGGQNKAIFVSLV
                     RSKEERRIDETVAPTEIGFLQDIRRLNVALSRAQNSLFIVGDFDTLSNARRPEAAKTF
                     AAIRAFYQKEVAEGAYAAAAQAPSVSERHLVRALSVVPVFPSMIETVRGFVPPADSTV
                     VVPLIIALVATLAFAAGFLLLVRSVVPAILRGLKSLAGWTLERFSRIPFGVRLWPNRL
                     IQDQTWAQALTAEPVLPVGLEGRGYPSLAGLQHDVFMAGRDVLKNVKLASGPLIPYDR
                     SDAGTLRVSVSGASVTLTSSAAFLDSLQQKLSMPDRLGRLLGRAADLLHLRALASTPA
                     RWRRSLLSALQHYGNPSLDDLQNLLLSPILENPEQREYLRLAIQQEAAGAFGQLRGVS
                     VEMWSDRVLRDPTLFESIGDNALKTALVRAMFEKARIWRLQTPQGEESVPLAVLAQRR
                     FLNLSLPNPPAVRVVSGTEIFRAHRPGAARTDASQDPYELYLEDEHVILLNGDRLKTF
                     DDYLAYYLHGLGHATLSQAMKTLGLAEKQNPQTRLIHEGWAEDFLQSTLQSLAGAYRE
                     PATGRSAGYDLVALIHQLRLYRSTESAPEALAGMALFQTLREFVDETTWYERVLPALY
                     EIMGGRKDIADLLQTLPNRPGVGSAETADGQDLGAVLRSRLLRAGEDLAAGFAPQSLR
                     SLTSPRIAADETAAGQEPVSPAVEWAKSQNPHEPLTALPELAVPNLAAGELRVVGFSA
                     DSFLEEIWSAGKNKVGVRFETLGVLSTLEQMAVTAGPAKRLQFVLYYDMNRIPMDSDV
                     LLNTILALAPGLKDSLLIVSSEDVRKAKGNLVDAMTQVLQQNNRLTSDSPKFEFVLTD
                     KAQLEMWEAALSDEQRSQIRWILLMPKELQNQVLSTALGVAIGVHGSSAEWLRNYIKA
                     TYKGAAAQVLESLERSRNDGQRYLELPAREAEDSYLDKLGEERIIDIQA"
     gene            12572..12889
                     /locus_tag="P5079_03640"
     CDS             12572..12889
                     /locus_tag="P5079_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29111.1"
                     /translation="MPVTANNMMGLVILSLGVLGIIISFLITDRKKYLVAMVLALLIV
                     VTGAYQYVSTGIRQWQTARRIASLQKQQRLNLEALQERLRQAQQQAVEKGGRPAPAAP
                     AKK"
     gene            13023..13955
                     /gene="nadA"
                     /locus_tag="P5079_03645"
     CDS             13023..13955
                     /gene="nadA"
                     /locus_tag="P5079_03645"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRY29112.1"
                     /translation="MQTAPPLSLAAEIQRLKKERDAVLLAHNYQIGEVQDVADFVGDS
                     LALSQQAAKTSARVILFCGVHFMAETAALLAPDKTVLLPVLDAGCSLASTIEVMQLRA
                     WKKAHPDALVVAYVNSTAEVKAESDYCCTSSNAVKVVESLPPDREILFVPDMYLGRFV
                     REKTGRKILLWPGSCHVHYKMRDFDIERMKKDHPRAEFLVHPECGCLSSALRFADHIV
                     STGGMLRRVKESPSKEFLVATETGILHTMKKQCPEKNFIPISESAQCEFMKKITLENV
                     LWSLQDMKHRVTVPPEVAEKARRAIDRMMAVPGV"
     gene            14092..14676
                     /gene="folE"
                     /locus_tag="P5079_03650"
     CDS             14092..14676
                     /gene="folE"
                     /locus_tag="P5079_03650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRY29113.1"
                     /translation="MTQKNTDPDKIARLVSELLSELGEKPGREGLVRTPQRVARSLRE
                     MTKGYSIDLDRLINGALFSEPYSQMVVVRDIKFYSLCEHHLLPFFGVCHVAYIPAGKI
                     IGLSKIPKLVKAFSQRLQVQERLTNEIAEIIQEKIKPMGVAVVMEARHMCMEMRGAES
                     LTSPTLTSSMLGIFRKDARTREEFFSLIHSRQSA"
     gene            14692..15129
                     /locus_tag="P5079_03655"
     CDS             14692..15129
                     /locus_tag="P5079_03655"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRY29114.1"
                     /translation="MPIQEGQKAPTFTAKTDDGKTISLSDFQGKNVVLYFYPKDDTPG
                     CTKEACAFRDKQTDFGGKNAVILGVSLDGAASHVKFKQKYGLPFTLIADEDAAVSKAY
                     GVYLADKKRAARWTFVIGPDGLVKRIFPDVSVDGHWQEVLNAL"
     gene            15119..15799
                     /locus_tag="P5079_03660"
     CDS             15119..15799
                     /locus_tag="P5079_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY29115.1"
                     /translation="MPSSRKSAPPAVLSVDAILFDLDGTLVDTIEDITLSLNHALARL
                     NKPPKTPPEVARFIGDGIHELVHRALGNNGDTAALEEGIRLFKPHYDAHCADHARLYP
                     GVLEILNHYADKKMAVVTNKLEEPSRRILQALKIDRFFPVLLGGDSFSERKPSPGPLL
                     EALRRLGVPPEKSVMVGDSPGDVVSGKAAGTFTCTVSYGFRTAEELRRTRPDIFLDSL
                     AGLRRRLR"
     gene            15915..15991
                     /locus_tag="P5079_03665"
     tRNA            15915..15991
                     /locus_tag="P5079_03665"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15949..15951,aa:Cys,seq:gca)
BASE COUNT         3255 a         5554 c         4453 g         2730 t
ORIGIN      
        1 ctcggaacgc tcgggcgaag tagtcttcaa taccgccatc accggctatc aggagaacct
       61 gcaggattgg gtcaccggcc agcgggaatc catccaggaa tcctgggacc ggctggaggc
      121 ctatcgccag gagatccttt cgggcggcag cgcggggggg aacaccatca ccccgggaga
      181 acagaaacag ctggccaacc tggaatacca gtggaacaaa aaagtctccg agttccaaca
      241 agcgcttccg ctgcgggaac aggaactggc cacccgcatc gcgaaagcga acgactccct
      301 gcaacacaat caggaaatgc tcaacatcgg ccgggagatg gcctccctcc tgccggtgaa
      361 agccgaagtg agcggggtca tcggcctgct ggacaaactc ttcaaccaca agctctactc
      421 caacgccgcc cgcctccgca tggcgcagga cgtgggcaac ggagtggact ccatgttcct
      481 ctccatggaa aagaccctgg cctccttcgc ccgggagaac atcgacgcgc tccggacggg
      541 aagccaggtg gacctgatct tcagcggcgc caacacgccg cagcaattcg ccgccgggat
      601 gggccagctg accgacgagc tggtgcgcat catggaaacc atgaaacggc aggccctctt
      661 caacgccatc ctcaccaaag gccgggaacc cacggtccag gaactggagg ccatcagccg
      721 ggatttcgcg gaatacgaaa aggaactgaa gagcgcctac ctggagggtt ttggccaggc
      781 ctccgcccag aacgtggaca gcctccttct gcgctacctt ggccgggtcc agaccgtctg
      841 gaacgaggcg ctgcaacagg cggggaaatc ccccaagatc gcccaggagg tggacctgca
      901 gcaaatcttc aagcagaccc tgctgcgcga tcccaaacag ggaccggtgg ggatggaaga
      961 ttacaaactg ggcctgcagc gcgccgtcat ggccgccacc agcgccatct cggacccgct
     1021 ccaaatgacg ccgccgggag cgcccctcgt gcaggaactg gagaccgtcc ggcccgacat
     1081 gctcgccgaa agcgtggacg cccagaattt cttccagaaa gacgtccaga tgttccactg
     1141 gtacatgatg cgctacatcg cctcggtgcg gccgaattac aagatggacc cggcgctgga
     1201 gaatttctgg aaagacgccg cctcgctctc cttaaaggac aagcaggccc ggctcgaaca
     1261 atacgcccag ctcctggacc ggctcctgca ggacatcaac ccctcctatt ccaaaggaac
     1321 gctcaccacg ctgctttaca actccctgcg ctcttcttcg gacaaagtcg gtgtctggca
     1381 gccggaagac gtcatcaaac tggggttcct gccgctgatg cagttcatgg accggcaatt
     1441 gaccaacttg aacaacctga acatgctgga gggaaaaccc ttcccggccc tggcggaaga
     1501 cgtcatcacc ggctcctacg tgcgggacgc ggcggccgcg ccccgggcct tcaccgacgg
     1561 catggtggcc tccctgcgtc tgctgaacca gatctatggg atggacacga acacgggcac
     1621 ggcggagctc caggcgaaac tccaggaaat cttcggcgct ccgcgcgacg tcaacgacct
     1681 catgccgcgc ctggaaaatg ccgtccgcca gatggacgag cttctcggcc aacgaatccg
     1741 ctcccaggga gggtactacg aatccggcac gctggtgcgc tcgttcaacg acgcttatcg
     1801 cggcgtcccc caggtggatc tgggaaccgc ccgcggcgtc tacaaccgcg tgatggacga
     1861 tctgcaggac atcctctcca aagcctacac cgtccgcaaa gacaacctca ccgtgtacgg
     1921 ctccatcccg gatggggtca cgctggagca gttcgggagc gaggaacatc tggggaaatt
     1981 cctgcaaggc atgctcctgg acctcacccg cctggaacag atcgccctct acaaccatcc
     2041 ggaaaaccgg gcactgatcg aacagacgtt caacggaatc caacgggaca tctacgccgg
     2101 ctatcggtcc tctttcatcg attccaaacc cgacgacatg atcgacctca tcggacgggt
     2161 ctccaacctc ctccggtcct atgagggggg actgcggcag gccgtccagt gggtggacaa
     2221 ggaccacgcg gtgcggcacg gactgctgga cgacgccttc atggaaaaag ctctcggcga
     2281 acaaggccag cgcgacggcg cgggagaacc caccctcaac gacctgaaga aagggctcca
     2341 acacgccgac gacgccgccg acagcctcat ccaacaggtc tccaccaaga tcacgcggga
     2401 acacgacgtc ctcctctccg ggcagacgcg tcgcgatttc ttcgccaacg atttcttcgc
     2461 cgaccctcag gtcatcgaag cgctccggac ggatgtggcc cgttcgcaca aagtcctcct
     2521 tcaggggatc cgcctggtct accagaccga cgacaccaag gcgctcatcg accccctcca
     2581 gaacgcgccg gacgtgtccg gcctccgcaa cgccacgctg gaccaggcca agcagtgggt
     2641 ggtggattac gcccgccgcg ccgacgatgt cctccagacc gtgggcgttc gttattccgc
     2701 cggaatgctg ccgaaacaga tcgaggaaag catgatgtcg gcccgctccc ccggcgaagc
     2761 cgtgcagatc ggcatgagcc ggatgaacgc ccttctctcg gaacatctca agagcgtctt
     2821 cacctggaag aaccagtggc gcatcctccc ggacatcgcc gaagaaacca cggaaacgat
     2881 gcgatccgtg acctccgtct cccaagtcgc ggacgcggtc cgccaggacg tggaacgctt
     2941 cgtcgaagcc tacaaactgc tctccggcga ccaatccctg aacgcggaag agatcatcgg
     3001 gacgccctat caggggcagg accccgccgc cctcacgccc tggatggacg gcttggtgcg
     3061 ccgcctggag aacttcctga acgcgcaccc gatcccgggt atgtatcgcc agaaggggat
     3121 cctcttggaa gcggccgcca cggcgcggcg cggcgccagc ctgggcaacc ccctggccgt
     3181 ggaaaaatac gtgtataccg aagtcatggc caaagcccag tcggtcttgg aacagacctt
     3241 cttcacccgg gcgcagacga tcggcgcagc gatccacgcc ccgctccccg agaactaccg
     3301 gatgtcctcc aacatcgacc tggccatcgc cgattacgac atccacgaca acgacatcat
     3361 catcatcctg agcgaccgca cgcgggtacg gctctcgggc atccccacgc ccgcgaacaa
     3421 ggaggcctcc ttggcgctgg aaaacgccat ccgcgacgtc ctgcggcgtg gcaccaacgt
     3481 cgtgctcgtc accgaacgga acgagcgtgg gctgtccgtg accggccacc tctacctccc
     3541 ggcggaaacc acggccgccg cgcggtatcc cggcctctat atggacgcca acgccgtgtt
     3601 gcagggacgc ggcttcgggc accgctcgcc cgacgaaatt ctgcctctct ccctttacac
     3661 cacggacaac atggtttccg aggagcagct gctcaccaac atggaagcca cggccaaaaa
     3721 cctcgccgac atggcctact tcctctccaa aggggacaac cgcctccggg aggccatcct
     3781 ggcgctggcc caaaaacagt tcgggcgcgc ccggccggga acggacctca ccccggactc
     3841 gatgggcggg aaaatccaac aagttatcac ccggcccctg cgccatcagc ccgtcgagga
     3901 agtcgtgtcc cagggacggg tgaacgaacg ggtggaggcc ttcttccgcg aagtgctgcc
     3961 cctcctccgc gaagcgtcca accgcgcggg tggccatctg ctggaggccg accagaaaga
     4021 cctcgactac aacatgatga ccctcttcga aagcctgcgc gggagccacg gcgacatgca
     4081 gagcatcgcc ctctcttatc gttggggcat ggaattcctc agcaagatga tggtgaaacc
     4141 catggacatg tccgttcccg acctcgacag ggatttcaac gccgccatgg cgggcgtgca
     4201 ggacccggcc gatttcgcct atcaacaggg cgagatctcc gtgaaactcg agaaactgtt
     4261 ccgcgccgtg gccgaacggc acgggttcaa gatttccgac gagagtttcc aagccatcaa
     4321 cgaggccagc aagaaattcc agttcttggg ttccgaaggc gggctcacgc cgcaggtccg
     4381 cgcctatctg gaggccttcg accaggtgct ccgtcaggag ttggggaaga tgggctacgc
     4441 gtatcgcggc aacctggccc gggacgtctt cgatccggcc aacatcgccc gcctgggccg
     4501 gggcccgctc gaccatgtgt cctccctgtg gggaaaacgc tccgccaccc ccgacctgac
     4561 caccctccgg ggcgccatgc tcgcctacga gaacggcgtc aaccgcatca agaacatcct
     4621 ggccgacgcc aacagcgacc tcaccttccg cgcgctctcc ggccaatccg tgtccccgct
     4681 ggacgacgaa ctctccctcg aactgcggaa actggaggcc ctgccgtatc aacagaaagc
     4741 cgccctggtg gctctgggca tcgcgcctga aaccatcttc acccggcgcg accgcatcta
     4801 caaattcttc cgtgacaccg tcctgcggga catcctcatc tattccgtcg gccgcctcat
     4861 catccggctc accttcggcc tgctcttcgg ctttgtctgg tatgtgatcc tcctcacccc
     4921 ggccctcatc tataagaccc tcatcttcat ggcggctcac gaatgggggc ctttcaaagg
     4981 acgtcaattc gaaccggtca aagggctggc gggggtcaag gaatttctgg gcatgccgtc
     5041 cgtcttcagc ccttacaaat attccgtggt caaaatgttc agcgccaaga gcatggaaac
     5101 ccaaaaagtg cagaccgggc tcaagatcca cgaatccgtc tatcgcttct ccccgctttc
     5161 ccagaaatgg ctcctcttct gcgctcgctt cgccggcggg ctggactccc tgccgaaacg
     5221 caacatcgtt ctgtggatcc tctctcaggc gacggtccgt cccatcgtct gggtcatcca
     5281 actcctggaa ctggaccagg ccctcctcgg cttcggcctg atgtggacgg aagcgctctc
     5341 ctccatcggc cgcctcttcg gcggtccgac cgccaagacg gacgccgcca aacgtccctc
     5401 gcggatcaaa cacgggctct atcgcctgct catcccggta ttggccgtct tcatctccgt
     5461 cgccgggccg ttcttcatgt cgaaataccg tccggcctcg ccgctcgaaa cgctttcctc
     5521 catcgtcaaa tcggaacagg tgctggtgca gaaattcacc gcgccggcgc cggcgccggt
     5581 gcaagtcgaa cggccttgga cggccccgcc cgcttccgaa actgcgtccc aaccggcccc
     5641 ggaagccgca ccgactcaaa acgtcctgtc gtccgatgaa gctctgctgc agggcattta
     5701 cgccccggcc ggcgtctttc ctctcgacgt ccgcgccgtc accacgggac acgactactc
     5761 cttcacctcg ctgcccgacg gccaggcggt tctcaacgac gatttcggcg ttccccacac
     5821 caaacaccct ggcagccaac acgacggagt cgacgtcttc gccaacaccg gcaccccggt
     5881 gcgctcggtg gtggccaaag gcgtcatcga aaccgtcaac cggcccgtgg gcacggatcg
     5941 gggcacggtc gtctccgtcc gggacgacga aggcaacctc tacgtttatt atcacctggg
     6001 agccagcctc ccggccggcg tggcggtcgg cgccaaggtg gaaaaccttg ccggcggcgt
     6061 catcggttat gtcggcacga ccgaagacgc catgaacacc cacccgcacc tgcatttcca
     6121 gatcatcccc cgcggacaaa gcaaggcggt ctccccgttc ccgtccctgt cccattggag
     6181 ccagcagctc gacctcacga cgccgaccgc gccgtcggcg ttcggcaacg atttcagctg
     6241 gctgcctttt gtcaaccttc tgacgggcct gcaggacgct tccgacgacg tcatcgacgc
     6301 cgtccagggc gtcctgcgcg aataccgcct gcttgaatcc gacaccgaac cggaccaata
     6361 tctccaactc ctcaaacaac tgccaccgca tgcccatctg ccgtatgtgc tggcgcggtg
     6421 gatcctggaa aaagcccaat tcagcctcac cccggcggcg gcggacaccc gccacgcgcc
     6481 gctcgtccga cgcgccgtgg atctgacccg ccaggtgtgg ccggaatatc agccggacga
     6541 tttcgtcttc gtcgccggcg ggatggggaa atccctcggc aaacgcctct tcgcccgggc
     6601 gctcgacacc cggaccggtc ggacgaagat attggtccac gaagatgtcc ttcaggattt
     6661 ggaggcccgg gcgcggaaac tggccgaaac ctcgggggcc cagctgaccc aggtgttgaa
     6721 actcctgctg gtgggactcc tgcatgacct ggcggaatct tccggcgtct cccacgatga
     6781 gatcgagacg gccggcatga gcctgacccg gaacgattcc ctggcctacg agaacttcga
     6841 cgccatcctc cgggcggggg aagacctgcg gcgcgaaggc accggcacac cggccgaaga
     6901 aacccccgcg gtccttcctc cggcgcgcgc catggccccc gtcaagatcg tcagcgccgt
     6961 gctttacacc gcggcgatct ccggcctggc cgccgcgttc agcgccctct tcaccttgaa
     7021 cctgccggcc ctggtcctct tcggactgcc ggccttgctg gtggcggtgc cgttcaccgt
     7081 ccaagccgcg caatacctcc ggctgttgac ccacccgatg aacaagacct attttgagac
     7141 gggccgccgc ctgcgattca aaccttcgga cgaagccgtc atccgcgaag agtggcgcga
     7201 catcatcaaa cgtctttgga acacggacga cctggcggcc ctttatgccc gcttcttgga
     7261 agagaagaaa caattggaca ccgacctggc ggcgctgcac gaaacggccc gcggactcca
     7321 gggacttctc gccgtcaacc cacggcatcc ggtgcaccga caggaagcgc tccatgtccg
     7381 acaggaaatc ctccgcaagg agaacgaaag ccgcgtgctg cgcgcccgcc tggaactgag
     7441 cgggctggac aaattcatca tggaatcgct cccgccggaa acctcgccgc tcgttgcccc
     7501 ggcggacaag ctggcctact ggaccgtcat cgacatctcc aatttccaac gcgccgtggg
     7561 ttccctgcag gaggtcagtt tccgcgtcct caaccggtcc aacaccggct tccgctggtc
     7621 gctggcctac gggctcggac tcctcttcaa cggctggatg gggaaagtct cccaacggaa
     7681 agcgccgccg ttggcccaaa cgcgctgggt cacccgcaac ggacggctgg tcccctacat
     7741 cgaattgacc ccggcgctgc tcttgaaccg caccgccctg cgccaggccc tcgcccacga
     7801 atggggggaa tcccggaacc tcggcgaagc cacctccctg aaactggaaa accgcctggt
     7861 ccccgccgcc tggacgcaca ccctcttcaa cggtccgctg gcgtggctgg acgaaaaagt
     7921 gacccgcctc ctcggctggg agcccggccg ggtcgaacag atcctcggcc aactggtgat
     7981 gatcggcttc ttcctgtggg tcggttcgct cctcgccgtc aaatccggcg ccgcggtgct
     8041 gatgggggca accatcaaga caacggacaa tgtcgacttc tacgaaccgg agacagcccg
     8101 gacccggtcc caagacgaag tcctcggcct gctttcaagc cacgccgcca tcctgacaaa
     8161 aatcatcaac catctggtca aaacgggaac ccacgtggtg ggcatacaag attcgcgctg
     8221 ggaaaacggc aagctgcacc tcttcatctc ttccaggaac gcgcaggagt tctcgtcggt
     8281 cctgaagcgg ggagaaagtt tcgctctgag caatgacccg cgcgcgccca aataccgcat
     8341 tgagaaattc gatcattcga cgggccggct cacggcggtg ccggcaaacg acaacgcgcc
     8401 gtcccgcggc ccgccgttcg tcaacacgat ttatttcatc cccaacaccg cttcggaaaa
     8461 catccagcgg gaacggctgg aaaacatcgc ccgtgaaata cgcacgaacg gattgaccca
     8521 ttcgccgctg atcaatcatg tcctcggact cgccaaaccc gccaagccga tgactttgcg
     8581 caaggtggaa aattccctta aagccgtcca ctttttcgac aagaacgtcg agaacgatcc
     8641 gtcgcagcga atcgtcgtcg gtttgtcgct gcgaatgctc caacacccgg aacaaaagct
     8701 tctcctcgtg agaggccccc cgggaacggg caaaaccacc accgcccaag aaattatccg
     8761 tcaagccacg gcccaagggt tgcgcgtgct cctcgtctct cagatgaacc aggcggtgga
     8821 caacgccctg caagccttcg ttgattccga cttccccgcc ttgcgcgcca gcaacgatcc
     8881 gccggaaacg atgcgtgagt atgggacgag ccgtctctgg atgggcaatc cctccgaaga
     8941 ttcaggaacg gcccttttcc tccaacgctt cgccaaaaca gaaaagggcg gtttcgtcgt
     9001 cggcgcgacc aacgtgggcg ccgtcacaac gcatgtcaat cagaaactgg cccaattgct
     9061 caacaaaggg accttcgacc tcgtcatcat ggacgaagcc agccgcgaca acctcgccgg
     9121 cagcctcttg ccgctgaccc tggcaaaaaa ggccattatc atcggcgacg aacaccaatt
     9181 gccgacctac ccgatggcgg gcatgaacgc cggccaagcg tctcaagaac ttgacgttga
     9241 cgccccgcac tacagggatt tcaacatgag cctcttcgag cggctcctcg cctcgcggca
     9301 ggccaccgtc gccatgccgt cggtcaaccg ccgctcccat ccgttgatcg ccaaattcat
     9361 cagcgtcatc ttctatgcgg gccggctcga aacgaaaccc tgggaaacgg cgcggccgga
     9421 taccctggtc attaaggaca cctcccgaat ggggatgtgg gaggaaaaag acgcgaacaa
     9481 ttcggtcttc aacgtcgggg aggccggact gatcgtcgac gcctacgggg aacacctccg
     9541 ggccggcaac agcgccgaca atgtcacgat catctcccct tacatggcgc aaatcgccct
     9601 gatcagtcga atgatccgtc aacggggatt gcgggtcccg gaaaccacca ccgtggacgc
     9661 tttccagggc gggcagaaca aggcgatttt cgtctcgctg gtgcgcagca aagaggagcg
     9721 ccgcatcgac gaaaccgtcg ccccgacgga aatcggattc ctccaggaca tccgccgctt
     9781 gaacgtggcg ctctcccggg cgcaaaacag tctctttatc gtcggggact tcgacaccct
     9841 ttccaacgcg cgccgccccg aagccgcgaa aaccttcgcg gcgatacggg ctttctatca
     9901 aaaggaagtc gccgaagggg cctacgccgc cgcggcccag gccccttccg tttccgagcg
     9961 gcatctggtc cgggccctgt cggtcgtccc cgtcttcccg tcgatgatag aaaccgtgcg
    10021 tgggttcgtc ccgccggcgg attccaccgt cgtcgtcccg ctgatcatcg ccctcgtggc
    10081 caccctcgcg tttgccgcgg ggttcctgtt gctcgtccgc tctgtcgttc cggcaatcct
    10141 ccgcggtctc aagtccctgg cgggatggac gctcgaacgg ttcagccgca tcccgttcgg
    10201 tgtccgcctg tggccgaacc gcctcatcca ggaccagacc tgggcgcagg ccctcacggc
    10261 ggaaccggtc ctgccggtcg ggctggaagg acggggctat cccagcctcg ccggcctcca
    10321 acacgacgtt tttatggccg gacgcgatgt gctgaaaaat gtgaagctcg ccagcgggcc
    10381 gctcatcccc tacgaccgct ccgacgccgg gaccctgcgc gtctcggtga gcggcgcctc
    10441 ggtgacgctg acgtcgtccg cggcttttct ggactccttg caacaaaaac tttccatgcc
    10501 ggaccgcttg ggacgactcc tcggccgcgc cgcggacctg ctgcacctgc gagcgctggc
    10561 gtcgacgccc gcgcggtggc ggcgcagcct cctctccgcc ctccaacatt acggcaatcc
    10621 cagcctggac gacctgcaaa acctgctgct gtcgcccatc ttggaaaacc cggaacaacg
    10681 cgaatatctc cgtctggcca tccaacagga ggcggcgggg gctttcggtc aattgcgcgg
    10741 cgtgagcgtg gaaatgtgga gcgaccgtgt tctccgggac ccgaccctct ttgaatccat
    10801 cggcgacaac gcgctaaaaa cggcgctggt ccgggcgatg ttcgagaaag cgcggatatg
    10861 gcgcctgcaa acaccgcagg gcgaagagtc cgtgcctctc gccgtgctgg cccagcggcg
    10921 cttcctcaac ctttcgctgc ccaacccgcc ggccgttcgg gtcgtctccg gaactgaaat
    10981 tttccgggcc caccggccgg gcgccgcccg caccgacgcc tcccaggacc cctacgagct
    11041 ttatctggaa gacgaacacg tcatcctatt gaacggggac cgtctgaaga ccttcgacga
    11101 ctatctggcc tattatctcc acggtctcgg ccacgccacc ctgtcgcagg ccatgaagac
    11161 cctgggcctg gcggagaaac agaacccgca gacccgcttg atccacgaag ggtgggccga
    11221 agatttcctg cagtccaccc tgcagtcgct ggcgggggcg taccgcgaac ccgccaccgg
    11281 acgcagcgcc ggatacgacc tcgtggcgct gatccaccaa ctgcggctct atcgcagcac
    11341 cgaatccgcc cccgaagctt tggccggcat ggcgttgttc cagaccctcc gggaattcgt
    11401 cgatgagacc acgtggtatg agcgcgtcct tcccgccctc tacgagatca tgggcggtcg
    11461 aaaagacatc gccgacctgc tgcagaccct gccgaaccgg ccgggggtgg gctcggcgga
    11521 aaccgccgac ggtcaggacc tcggcgccgt gttgcgttcg cgcctgctcc gcgccggaga
    11581 ggacctggcc gcggggttcg cgccccaatc cctccgctca ctgacctccc cgcgcatcgc
    11641 cgccgatgaa acggccgccg ggcaggaacc cgtctccccc gccgtggaat gggcgaagtc
    11701 acagaacccg cacgaacctt tgacggcgct cccggaactc gccgtcccga acctggccgc
    11761 cggagaactg cgcgtggtgg gattctccgc cgattccttc ctcgaagaga tctggtcggc
    11821 cgggaagaac aaggtcggcg tccggtttga gaccttgggc gtgctcagca ccctggaaca
    11881 gatggccgtc accgccggcc ccgccaaacg gctccagttc gtcctctatt acgacatgaa
    11941 ccgcatcccc atggatagcg atgtcctcct gaacacgatc ctggcgctgg cccccggcct
    12001 gaaggattcc ttgctgatcg tcagctccga ggacgtccgc aaagccaagg ggaacctggt
    12061 ggacgccatg acccaggtcc tccaacagaa caaccggctc acctccgact cgcccaaatt
    12121 cgagttcgtc ctgaccgaca aggcccaact cgaaatgtgg gaggccgccc tctcggacga
    12181 acaacgttcc cagatccgct ggattctgct gatgcccaag gaactgcaga accaggtcct
    12241 ctccaccgcc ctgggcgtgg ccatcggggt gcatggctcc tccgccgaat ggctgaggaa
    12301 ctacatcaaa gccacctaca agggcgccgc cgcgcaggtg ctcgagtccc tggaacgctc
    12361 ccgcaacgat ggccagcgtt accttgaact ccccgcccgc gaagccgaag attcctatct
    12421 ggacaaactg ggagaagaac gcatcatcga catccaggcg taaaccctcg ccggccccaa
    12481 ccatttttta aagacccttg ttcaatcgcg gtttatctgt tatatttgaa ccgttgttga
    12541 cagttgtcag ccagaaaggg aggccgtacg catgccagtt accgccaaca acatgatggg
    12601 actcgtcatc ttgagtctcg gagtgctggg aatcatcatt agttttctta tcaccgacag
    12661 aaaaaaatat cttgtggcca tggttttggc cttgctcatc gttgtgacgg gggcctatca
    12721 gtacgtcagc acgggcatcc gccagtggca gaccgcccga cgcatcgcca gcctgcaaaa
    12781 acaacagcgt ctgaacctgg aagccctcca ggaacgcctc cgccaggcgc agcaacaggc
    12841 ggtggaaaaa ggcggcagac cggcgccggc cgcccccgcc aaaaagtaac gcatcttttc
    12901 acttcttctt aaaaagagga gacccttccc gggtctcttt ctttttttgt accccttccc
    12961 ggggatggat tcattcaaac ctgtccgctt aaaagcggta taataaaagg gaggacatgc
    13021 ccatgcagac cgcacccccc ttatccctgg ccgccgagat ccaacgcctg aagaaagaac
    13081 gcgacgccgt ccttttggcg cacaactacc agatcggcga agtgcaggac gtcgcggatt
    13141 tcgtcggcga ttccctcgcc ctctcccaac aggccgccaa gacctccgcc cgggtgatcc
    13201 tcttttgcgg ggtccacttc atggccgaga ccgccgccct cctcgccccg gacaaaaccg
    13261 ttctccttcc cgtcctggac gccggctgtt ccctcgcctc caccatcgaa gtcatgcagt
    13321 tgcgtgcctg gaaaaaggcc catcccgacg ccctcgtggt ggcctacgtc aattccaccg
    13381 ccgaggtcaa ggccgaatcg gattactgct gcacctcctc caacgcggtc aaggtggtgg
    13441 agtccctccc gcccgaccgg gagatattgt tcgttccaga catgtacctc ggccggttcg
    13501 tccgggaaaa aaccggccgc aagatcctcc tctggcccgg ctcctgccac gtgcattaca
    13561 agatgagaga tttcgacatc gaacggatga aaaaggacca cccccgggcc gagttcctgg
    13621 ttcatcccga atgcggctgc ctctcctcgg ccctccgctt cgcggaccac atcgtctcca
    13681 cgggggggat gctccggcgc gtgaaggaat ccccgtccaa ggagttcctc gtggccacgg
    13741 agacgggcat cctgcacacc atgaagaagc agtgccctga aaagaatttc atccccatca
    13801 gcgaatccgc ccaatgcgaa ttcatgaaaa agatcacctt ggaaaacgtg ctgtggtccc
    13861 tgcaggacat gaaacaccgc gtcaccgtgc cgccggaggt ggccgaaaaa gcccggcgcg
    13921 ccatcgaccg gatgatggcc gtccccggcg tctgaaccgt ccgccctccg ccagcgcctc
    13981 tccgccgtct ccgaagaatt gcgccgtgga tttcaacgag gagaaattgc taatatcttc
    14041 cgtcttcggg aacaaaacaa cgtcgctgtt cgttggacaa taaaggacgc catgacccag
    14101 aagaacaccg atcccgacaa aattgcccgc ctcgtgtccg agttgctctc cgagctgggg
    14161 gaaaaacccg gacgggaagg cctcgtgcgc accccccagc gggtcgcccg ctcccttcgg
    14221 gagatgacga agggttattc catcgacctc gaccgtctca tcaacggcgc cctcttctcg
    14281 gagccctaca gccaaatggt ggtggttcgg gacatcaagt tctattccct ctgcgaacac
    14341 cacctcctgc ctttcttcgg ggtgtgccac gtggcctaca tccccgccgg aaagatcatc
    14401 gggctctcca agataccgaa actggtgaag gccttttccc aacgcctgca ggtccaggaa
    14461 cgacttacca acgaaatcgc ggagatcatc caggaaaaga tcaagcccat gggggtcgcc
    14521 gtggtcatgg aggcccgcca catgtgcatg gagatgcgcg gcgccgaaag tctcaccagt
    14581 cccacgctca ccagttccat gctgggcatc ttccgcaagg acgctcgcac ccgggaggaa
    14641 ttcttctccc tcatccacag tcgacagtcc gcctaaccaa ggaggcccgc catgccgatc
    14701 caagaaggac aaaaagcccc gactttcaca gcaaagaccg acgacggaaa aacgatctcc
    14761 ctttcggact tccagggaaa gaacgtggtg ctgtatttct atcccaagga cgacaccccc
    14821 ggctgcacca aggaagcctg cgcgtttcgg gacaaacaga ccgacttcgg cggaaagaac
    14881 gccgtcatcc tcggcgtgag cctggacggc gcggcgtccc acgtcaagtt caaacagaaa
    14941 tacggtctgc cgttcaccct catcgccgat gaagatgccg ccgtctccaa ggcctacggc
    15001 gtctatctcg cggacaagaa acgcgccgcg cggtggacgt tcgtcatcgg tcccgacggc
    15061 ctcgtgaaga ggatattccc cgacgtcagc gtggacggac attggcagga agtcctgaat
    15121 gccctctagc cgcaaaagcg ccccgccggc ggtcctctcc gtcgatgcca tcctcttcga
    15181 tctcgacggg accctcgtcg acaccatcga ggacatcacc ctctcgctga accacgccct
    15241 cgcgcgcctc aacaaacccc ccaagacccc gcccgaggtg gcccgcttca tcggcgacgg
    15301 catccacgaa ctggtgcacc gcgccctcgg caacaacggc gacaccgccg ccctggaaga
    15361 ggggatccgc ctcttcaaac cgcattacga cgcccattgc gccgaccacg cccggcttta
    15421 tcccggcgtt ctggagatcc tgaaccatta cgccgacaaa aaaatggcgg tcgtcaccaa
    15481 caagctggag gagccctcgc ggaggatcct gcaagccctg aagatcgacc ggttcttccc
    15541 ggtgctcttg ggcggagatt ccttctccga acggaaaccg tcgccgggcc ccctgttgga
    15601 agcccttcgc cggctggggg ttcctccgga gaagtccgtg atggtcggcg acagccccgg
    15661 ggacgtggtc tcgggaaaag ccgccggcac gttcacctgc accgtctctt acggtttccg
    15721 caccgccgag gaactgcgcc ggacccggcc cgacattttc ctggattccc tggccgggct
    15781 gcgccgccgc ctccggtgac ctccggcctg gaaacgcctc cgctccgacg gacaatatcc
    15841 aagcgcgccg gaatcccggc acgcttggct tgtcaagcgc ccaggaactt cttaaaatga
    15901 aaagctgtgt ccgcggtgcg gtacccaagc ggcctaaggg ggaagtctgc aaaacttcta
    15961 ttcgccggtt caaatccggc ccgcacctcc aa
//
LOCUS       DAOTFD010000034        15874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2508_length_15874_cov_4.793666, whole genome shotgun sequence.
ACCESSION   DAOTFD010000034 DAOTFD010000000
VERSION     DAOTFD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15874
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_15874_cov_4.793666"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2869
                     /locus_tag="P5079_03670"
     CDS             <1..2869
                     /locus_tag="P5079_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29116.1"
                     /translation="LGAALLLAAGPARAFGKNKVNVHDFRWRVVSTEHFDIHFTAESE
                     PLIPRAAFYLEKAYKTVTGDLDVPLKERIPFFFFVNHNQFEENNIVELGEGTGGVTEA
                     FKNRFLVFNDGTEFWMAHVIPHEFTHVAQFEVLYGGFWKSARLLKSPLYPLWFMEGLS
                     EYETGDLDVVTEDLYLRDAATSGRLYSLLQLHGFSHLRPHEVTLGYKQGGAAVRFLAE
                     EYGPEKVAETLHLLRDRFEVPSIITDLTGQDFRAFDRRYREFLADYYALQSEGTREPE
                     TDGRRLTPDDLLPVFNTHPAFSPDGRFVAYLTDRNAAQEVALYDVKAGTHRSLAGRQW
                     NRVENIHADGRAVSFSADGRWLAFVGEKTQRDYLYLYDVKRDRLRRVRTPFEQVRSPV
                     FHPSEPRLALVGMKDGTNDLYEVTPRGKVLRRLTDSPADESAPAYSPDGKMMVYSAET
                     AAAGPHGAPARDLAALNLATLSTAALTHLPWNETSPVFTPDGRAVVFVGEADGIPNLY
                     RLDLPGGAVSRLTRVIGGNFSPDISRADNTIVFSSYRRGSQNLYLGDETLWSPSAPKE
                     PIASSQPASANWAALAGGTPSPGSPAASPAPGREPAPPETAVGPFQTSTLFVGADRPY
                     RFRASTDLFYPLFFYSSTDGLFLATYWQASEYLGNHQLQTAFQYSSGNDFLDYQAQYV
                     YARFRPQFFLLAGGQNYFRDFAETEFRKETEQAVGVSYPLDRFNRTEAVAGSVFKEDR
                     YEDFPEFNARERENFSAVSFVRDTTTGRYLATTAGRRFRATYQVARPVFGGDRDYRTQ
                     VFEYHNFSPTGGESALAFRGLGGVSVGDSPQLLRLGGVDRLRGFSRNDDENHSSRFLL
                     TNLEWRVLLKYLDVRTGFIFPDFYFKAVYGAVFTDNGYAWDRSSDLNSLQAGRIKNSV
                     GAGLRVPMFIMQTYVLNLSLDVAKRTDASSWVWYFSLGPVF"
     gene            3004..3291
                     /locus_tag="P5079_03675"
     CDS             3004..3291
                     /locus_tag="P5079_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29117.1"
                     /translation="MTKKMKTPPPAVHPRPVSLSEPILSKRGKIVIAAGIVTAAVGFF
                     LVTFTDPAGQNWASNLCPFVILGGYALIGVGIVVPDPAPPSSPPNPPLPQR"
     gene            3415..3810
                     /locus_tag="P5079_03680"
     CDS             3415..3810
                     /locus_tag="P5079_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29118.1"
                     /translation="MPPSRLFHAKKAARWMALWVFALNALLPGEARAPIDTVFSRADR
                     PAWTAGALEKHPYVFQRVIRNYFFASRLSSRRATRQERGVLDTFVRGQKGEGSAMGRQ
                     RFMFRAVLFFYPLTRLLAPPGADSDQPLG"
     gene            3925..4470
                     /locus_tag="P5079_03685"
     CDS             3925..4470
                     /locus_tag="P5079_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29119.1"
                     /translation="MKNVPSKKHLMDHIGDLQKELFDKEGLKLEVEWLEQALRKRTRL
                     LGERAKELGCIVRALKTLRDPRLPVEQKMQELVDFLPRAWQYPQNAGARIQVLKRTFK
                     TANYSETPWRQKCDIVVDGKPQGFAEVAYLCEKPEAEEGPFLREERMLLDVVCGCVGM
                     YLERDSGALLPPDRAGDVPYA"
     gene            4472..4912
                     /gene="rnk"
                     /locus_tag="P5079_03690"
     CDS             4472..4912
                     /gene="rnk"
                     /locus_tag="P5079_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRY29120.1"
                     /translation="MKKDEEETMSTRDIYLTDFDAKRLNDLLLGGQGVNPMDEKHVRE
                     LKKEIDRGHIVPSKDIPRDVITMNTKAKVRDLVTGEEFVYTLVYPKDADIREGKISVL
                     APVGTALLGFRAGDEIEWMVQGVPRRLKVKEIVYQPEAAGNFHL"
     gene            4934..5116
                     /locus_tag="P5079_03695"
     CDS             4934..5116
                     /locus_tag="P5079_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29121.1"
                     /translation="MLIQLMMRQMMEKSKKLASRSGGGGPSGPGPSMSPALRWGLIVL
                     AVLVAGFLFAVALRLI"
     gene            5179..6633
                     /locus_tag="P5079_03700"
     CDS             5179..6633
                     /locus_tag="P5079_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRY29122.1"
                     /translation="MSIEQIVILAAVALVASVMLSKVSERFGVPALLLFLGLGMLAGS
                     DGPGGVYFDNARLSQALGTVALAYILFAGGLETDVRSMRTVLKSALSLSTLGVFVTAL
                     VTGACAKWALGLSWLEGFLIGSIVSSTDAAAVFAVLRSKNVGLKEPLRPFLELESGSN
                     DPMAVFLTIGFIGLIKNPDRSMASMVPLFFQQMVLGLLFGYVMSKLFIWVINRIKLDY
                     EGLYPVLCLGWVLLIYGFTAAAGGSGFLAVYIAGIILGNFTFSHKKSLMRFQDGIAWL
                     MQIVMFFTLGLLVFPKQLIAVAGQGLLMSFLLIAVGRPVSVFLGLALSRFNLREKLLI
                     SWVGLRGSVPIVLATFPLQAGLDQNHFIFNLVFFIVITSVLLQGTTIPLFARWLGVDA
                     PLVRKRRYPIDFEDSENTDMQLVDFIVPYGAKAAGQPILKLGLPKDSLITLVSRNEEF
                     IMPSGETVLEEGDVLLMLVNKTSLPAVRRILEAR"
     gene            6668..8353
                     /locus_tag="P5079_03705"
     CDS             6668..8353
                     /locus_tag="P5079_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY29123.1"
                     /translation="MDHSSVTLLTDIALGIIFAALAAHAIRLFRQPLILGYVLGGVLL
                     GSHFGFGLVTNEQSIELISEIGLILLLFIIGLEINLRDLVKMGRSMFTLGIVQFAACV
                     LLGLAFFRPMGHGLGDGNFDLLYLAVALALSSTLIVVKLLHDKFEIHTVSGRLTVGVL
                     VLQDIWAIAFMAFQPNLLDPQLASIVKSLGLGAVLLLASFAASRHLLSRLFQSANKSP
                     ELVLITSMAWCFAVSGLAERAGLSKEMGALIAGMSIAAFPYGADVIAKVSGVRDFFVT
                     LFFVSLGIKVPAPTWQVAGTSFLVAGFVVLTRLLSVVPTLYLLGSGLRNGLVTALNLS
                     QISEFSLVILSLGAGYGHISDGLSSTVLSAMLMTSILATYIIKYNDALSRRLMRALAW
                     AGVRERHGHREGSLPDPSTKKDIVLLGCFRAGKALLETIEERAPHLKERVLVVDYNEA
                     LKGDLESRGFQWAYGDLAHPETLQHLGIDRAAFIICSLSDTFLKGITNRRLMKHLRHM
                     APAARLIMSAEEKHEAEILRREGAQHVIVVGKLSGMHIFEKLTENLGKRATFP"
     gene            complement(8453..10330)
                     /locus_tag="P5079_03710"
     CDS             complement(8453..10330)
                     /locus_tag="P5079_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY29124.1"
                     /translation="MAKPKHVLILSNRAEVLERIYDTPVSSAVTLTVAPGLNEFAQLS
                     QRYPFHGHILDMDLTEKQTWQIFQDTKLRQPTAFFSFLMGKTPEEGLQDMLNGAAGWF
                     VLYPPYPVDKLEQLKELLPKQPTILFDPPDPPAGKNFAEIRPFFKKADTVTAATLPAV
                     AEALAKNPKALAVLRVPRTETSLGAFLRDAGHDRPDARLLVVCDQPLDEASRTAVHSS
                     GWVAVPAPHGVPYLLDRLQESHARAETDRPPTERILVVDDEPNILDFVVDILTEHGYE
                     VDGYPSGTTAVAAMKSKEYHVALVDFQLGDMTGLTLSRELRQIDPDLNVILMTAHASL
                     DMAVKAIQADVYDYLIKPVDTNHLKRSLGKALEKRRLALEIKALVADLQKANHQLNRL
                     NDLKSRFLSIVTHDLRTPLTSIKGYAQVLTMQKTLPSEQQQHFLKIIAHETDHLAGLI
                     SDLMDFVSIEAGKLRVEKTSGSIVELVEAVGNRMASLAEQRKIVFQVEADPAALPELL
                     MDKRRIDQVLTNLVSNAFKHTPEGGSVKVRAETQKSDVRLQVIDSGEGIPPGDLPRIF
                     EQFYQVESHASKRDGIGLGLAIAKEIIQAHGGEIGVNSDGVGRGSCFWFTLPIPEKSG
                     N"
     gene            10591..12186
                     /locus_tag="P5079_03715"
     CDS             10591..12186
                     /locus_tag="P5079_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRY29125.1"
                     /translation="MTMPATSTRPAAPVDTMPEPVSPRVLFFSPVRSVSDAVGGMLQA
                     AGCQVAFCDSESLLPDCLAAGPWDIFFGDAPPAFFPAFFDKVASVFPAARGWLLLPDG
                     VEAKSAEKTFERSERRFRLFERPVTLNALLRALEEDTGVGREGAVPDEARVLIVDADP
                     VFCETLLDFLATEGYRGRTARSGREAIDEFRRQFYHIILLDSRRDDMSVEELARRLHE
                     VNPHAMVIEMADFASLDRVLHAMRADVHDYLLKPLDPLALRRTLHNALEKQKLGLEIQ
                     SLLGRLSRANRDLVRVNELKNQFLRIVSHDLRTPLTAVKGYAQALKAGMIPPAKYERC
                     FGTMSRECENLEHLIGDLVDFVSIEAGKMRLEKAPTDVHSLFEGIVERFRDIMEGKKI
                     RYRVEGFERTYPPVSADPRRMDQVLSNLVGNAAKHTPEGKSVTVQFRRAGDFLEVRVV
                     DTGEGISPEHAGRVFDQFYQAGPAMVQKEGLGLGLAIAKEIVGRHGGKIGVHSDGLGE
                     GTTFWFTLPFMPAAKGKSAGGTL"
     gene            12183..12572
                     /locus_tag="P5079_03720"
     CDS             12183..12572
                     /locus_tag="P5079_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29126.1"
                     /translation="MTARILIAEDDPNILNLLYVFLSQQGYTVEKATDGREALRLAAA
                     ITPDLLITDVMMPHMNGYQLVNAIVYERVDIPTPKIIMLTSRTDPSDVQRGLDVGVDM
                     YIPKPFDIAEVTARVAELLSEGKAKKD"
     gene            12576..14276
                     /locus_tag="P5079_03725"
     CDS             12576..14276
                     /locus_tag="P5079_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY29127.1"
                     /translation="MRKLKIFWMNHWVKIVVTFLIVAMVILTILGLASLESFYRNLTL
                     ATIPLQLLLTALNAAVFVYFYMSVTKGSFGKLRKNISVKAENVHISFKDVIGLENAKK
                     EAMEVVQLLRDRARLKAIGGRIIKGILLVGPPGTGKTLLAKAIASEAGIPFLSMAGSE
                     FVEVFVGVGASRIRSLFNQARQLAYAHGASIVFIDELDVIGRGRQFDAFGGAQETNST
                     QNQLLVEMDGLGEKEENVVVIGATNAGEEVLDRALLRPGRFDRKIYIDRPNLDERVDL
                     FRYYLKRVKYDPEIDIGRLARKCVYKSPADIMNVVKESALIATRNDRNVVTYKDLSQA
                     IERIDLGVVHRLNMTAHEREAVAFHETGHLVVLYQLHPTDDVFKASIVARGGALGVVH
                     HQPREELHTSSREKILADIKVALGGYVAEKVRYGVTSNGVASDFQAATRMAHAMVWRL
                     GMGTNGYVGDFSSMPENEISEHLKENLNKETYRLLSQCAKDVEELLRKEWALVERFVK
                     ELLEREELEYDDIHAIFTEYGKARSQVPIQTVHPDDAAPSASVPPPAAPAGPPPPSAS
                     "
     gene            14288..15181
                     /locus_tag="P5079_03730"
     CDS             14288..15181
                     /locus_tag="P5079_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29128.1"
                     /translation="MRRRSPLITFLPIGRALLWPALFSLTGVACLNRPTYTKDDLAAS
                     VQRLCQKEYGFKAEARLVGKTLYVVTALDGLVGRDLGLQRETMEKLEGAVLSSTRVAL
                     STNADLNYLVVKARDSRLGVTVTILRYLPDVKSLLYMRISRSDFMDRQVQETENALEP
                     ESPESWHDVTMMEFQARLVASRLQRLFSENPLIAVFLQVRRVEGVFVDGTLSLRLDKF
                     ENEPGTNFLVEEILRTAVEDTVRDVVKKYNLRGILKHVRVSDDADRRLLDLEVEELMS
                     RPPSPAPPKGVFSKLREKEAE"
     gene            15178..>15874
                     /locus_tag="P5079_03735"
     CDS             15178..>15874
                     /locus_tag="P5079_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY29129.1"
                     /translation="MKNPKEAKSKPVAAGKAAEAVAVRQRLLGVLLRKGTWPMYGFFK
                     EALQGIQRVAFSQGYGLVIVDPAPQGEISLRDAIPALRRTLAGLLMVAPPESDMAVLR
                     PLTDEQFPVVSLYRRFAGMNYVEVDNEGGAEAAVEHLIHLGHTHIAFLSGPADAVDGQ
                     ERLSGYQKALAKNGLTVHKEYVVAADFEQAKAYKAMKDLLALKKPPTAVFAANDYMAL
                     GAISAIHDAGLSVP"
BASE COUNT         2736 a         4957 c         5143 g         3038 t
ORIGIN      
        1 cctgggcgcc gccctcctcc tcgccgccgg gccggctcgg gccttcggca agaacaaggt
       61 caacgtccac gattttcgct ggcgcgtggt ctccaccgaa catttcgaca tccacttcac
      121 cgccgaatcc gaacccctca tcccccgcgc ggccttttat ctggaaaagg cctataagac
      181 cgtcaccgga gacctcgacg tccccctcaa ggagcgcatc ccgttctttt tcttcgtcaa
      241 ccacaaccag ttcgaggaga acaacatcgt ggaactgggc gaaggcaccg gcggcgtcac
      301 cgaggcgttc aagaaccggt tcctggtctt caacgacggc acggagttct ggatggccca
      361 cgtcatcccg cacgagttca cccacgtggc gcagttcgag gtgctctacg gcggtttttg
      421 gaagtcggcg cgtctgctga aatcgccgct ctatccgctc tggttcatgg aggggctgtc
      481 ggaatacgag accggcgatc tggacgtcgt gaccgaggac ctctacctcc gggacgccgc
      541 cacctccggc cggctctatt cgcttctgca actgcacggg ttcagccacc tccggccgca
      601 cgaggtgacg ctgggataca aacagggcgg ggcggcggtg cggttcctcg ccgaggaata
      661 cgggccggag aaggtggcgg agacgctcca cctcctgcgg gaccgcttcg aggtgccgtc
      721 catcatcacg gacctcaccg gccaggattt ccgcgccttc gaccggcgct accgggaatt
      781 tctggccgat tattacgccc ttcagtccga ggggacccgg gagcccgaaa ccgacggccg
      841 ccggctcacc ccggacgacc tcctgcccgt gttcaacacc caccccgctt tttccccgga
      901 cggccgtttt gtggcctacc tcaccgaccg gaacgccgcc caggaggtgg cgctgtacga
      961 cgtgaaggcc ggcacgcatc gttcgctggc cggccgccag tggaaccgcg tggagaacat
     1021 ccacgccgac ggccgggccg tgtccttttc cgccgacggc cgctggctgg ccttcgtcgg
     1081 cgagaagacc cagcgcgatt acctctacct ctacgacgtg aagcgcgacc ggctccgccg
     1141 cgtccgcacg cccttcgaac aggtccgttc ccccgtgttt cacccgtcgg aaccccgcct
     1201 ggcgctggtg gggatgaagg acggaaccaa cgacctttac gaggtgaccc cgcgcgggaa
     1261 ggtgctccgc cgcctgacgg attccccggc cgacgagtcc gccccggcct attcgcccga
     1321 cgggaaaatg atggtctatt ccgccgagac ggcggccgcc gggccgcacg gcgccccggc
     1381 ccgcgatctg gcggcgctga acctggccac cctttccacc gccgccctga cccacctgcc
     1441 ctggaacgag acctcgccgg tgttcacccc ggacggccgc gccgtggtgt tcgtgggcga
     1501 ggcggacggc atccccaacc tttaccggct ggatctgccg ggcggcgcgg tgagccggct
     1561 cacccgggtg atcggcggga atttctcgcc ggacatctcc cgcgcggaca acaccatcgt
     1621 cttttcctcc taccggcgcg gttcgcaaaa cctctacctc ggggacgaaa ccctctggag
     1681 cccctccgct ccgaaggagc cgatcgcctc ctcccagccc gcctccgcga actgggccgc
     1741 gctggcgggg ggaacgccct cgccgggttc tcccgccgcg tcgccggccc ccgggcgtga
     1801 acccgcgccg ccggaaacgg ccgtcgggcc gtttcaaacc tcgacgcttt tcgtcggggc
     1861 ggaccggccg taccggttcc gggcgtccac cgacctgttc tacccgctct tcttctattc
     1921 ctccaccgac ggccttttcc tggccaccta ctggcaggcc tccgaatatc tcggcaacca
     1981 ccagctgcag acggcgtttc aatattcttc cgggaacgat ttcctcgatt atcaggcgca
     2041 gtacgtctac gcccgtttcc ggccgcagtt tttcctgctg gcggggggac agaactattt
     2101 ccgcgatttc gccgagaccg agttccgcaa ggagacggaa caggccgtcg gcgtcagcta
     2161 tcccctggac cggttcaacc gcacggaggc ggtggcgggg tcggtcttca aggaggaccg
     2221 gtacgaggac tttccggaat tcaacgcccg ggaacgggag aacttttcgg cggtgagttt
     2281 cgtgcgggac accaccaccg gccgctatct cgccaccacc gccggccggc ggttccgcgc
     2341 cacctaccag gtggcccggc ccgtcttcgg cggcgaccgc gactatcgca cccaggtttt
     2401 cgaataccac aatttctctc ccaccggcgg ggagagcgcg ctggcgttcc gcggactcgg
     2461 cggcgtcagc gtgggcgaca gcccccagct cctgcggctc ggcggggtgg accggctccg
     2521 cggcttctcc cgcaacgacg acgagaacca ttcctcccgg ttcctgctga ccaacctgga
     2581 gtggcgcgtg ctcctgaaat atctggacgt ccgcaccggg ttcatcttcc ccgattttta
     2641 cttcaaggcg gtgtacggcg cggtgttcac cgacaacggc tacgcctggg accgttcctc
     2701 cgacctgaac tcccttcagg ccgggcggat caaaaactcc gtgggcgccg ggctccgcgt
     2761 gcccatgttc atcatgcaga cgtacgtgct gaacctctcc ctcgacgtgg ccaagcgcac
     2821 cgacgcctcc tcctgggtgt ggtatttctc cctcggcccg gtgttctagg gggcggtgga
     2881 gtcgacggcg gccgatggcc cggactccgt ccggctccct tcaaatggag gttgggacgg
     2941 gcgatcggcg aaccgcgggt tcgtcccttg acagggattc cgccgatata ggacaatgaa
     3001 tttatgacca agaagatgaa gacgccgcct ccggcggtcc acccgcgccc ggtctcgctg
     3061 tcggaaccga tcctctccaa gcgggggaag atcgtcatcg ccgccggcat cgtgacggcg
     3121 gccgtcggtt ttttcctcgt cacgttcacc gatcccgccg gacagaactg ggccagcaac
     3181 ctctgcccgt tcgtcatcct cggcgggtac gccctcatcg gcgtgggcat cgttgtgccg
     3241 gatcccgccc cgccgtcgtc ccccccgaac ccgcctctcc ctcagcgata ggaattgtcg
     3301 acgggtatca gcgttccgtc gcgggccgcg ccaaaacgat cttttcgccc aaggaagttc
     3361 tttagaggta taattcagcc gatgaacaga aaaaaatcag tccatcgaga agcgatgccg
     3421 ccgtcacggc tctttcacgc gaagaaggcc gcgcgctgga tggccttgtg ggttttcgcc
     3481 ctgaatgcgc tcctcccggg cgaggcaagg gcgcccatcg acacggtctt ctcccgggcc
     3541 gaccggcccg cttggacggc cggcgcgttg gaaaaacatc cgtacgtttt tcaaagagtc
     3601 ataaggaatt atttttttgc atcacgcctt tcttcccgtc gggccacccg gcaggaaaga
     3661 ggcgttctgg acacctttgt ccgcgggcag aagggggaag gttccgccat gggccgtcag
     3721 cggttcatgt ttcgcgcggt cctcttcttc tacccgctga cccgcttgct cgctcctccc
     3781 ggcgccgact cagaccaacc tctcggctga caagccgact tccccacacc gcgcccccgt
     3841 tccgtcgggg gaaacacgcc gcctctcctc ctccggcggg catccggaca ttttaacgag
     3901 aaaaaagaag aacggaatcg ggccatgaaa aatgtcccat cgaagaaaca tctgatggac
     3961 catatcggcg acctccaaaa ggagcttttc gacaaggaag ggctgaaatt ggaggtggag
     4021 tggcttgaac aggccctgag gaaacgcacg cggctcctgg gggaacgggc caaggagttg
     4081 ggctgtatcg tgcgggcgct gaagacgttg cgcgatccgc gccttcccgt cgaacagaaa
     4141 atgcaggagc tggtcgattt ccttccccgg gcgtggcaat atccgcaaaa cgccggcgcg
     4201 cgcatacagg tcctcaagag gacgttcaaa acggcgaatt attcggaaac gccgtggaga
     4261 caaaagtgcg acatcgtcgt tgacgggaaa ccgcaggggt ttgcggaggt ggcgtatctg
     4321 tgcgaaaaac cggaagcgga ggaggggccg ttcttgaggg aggaacggat gttgctggac
     4381 gtcgtctgcg gatgcgtggg aatgtatttg gaacgagaca gcggcgcgtt gttgccgccg
     4441 gaccgggcgg gagatgttcc gtacgcctaa gatgaagaaa gacgaggagg agaccatgtc
     4501 gacgagagac atctatctga cggattttga cgccaagcgg ctgaacgatc ttctgctggg
     4561 cggccagggc gtcaacccga tggacgaaaa acacgtgcgc gagctgaaaa aggagatcga
     4621 ccggggacac atcgtgccgt cgaaggacat cccgcgggat gtgatcacca tgaacacgaa
     4681 ggcgaaagtg agggatttgg tgacgggcga ggagttcgtc tatacgctcg tctatccgaa
     4741 agacgccgac atccgggagg ggaagatatc ggtgttggcc cccgtgggaa cggccctttt
     4801 gggtttccgc gccggcgatg agatcgaatg gatggtgcaa ggcgttccgc gccgcctgaa
     4861 ggtcaaagag atcgtgtatc agcccgaggc cgcgggaaat ttccatctgt aatatccggg
     4921 aagaggccga acggtgttga tacaattgat gatgcggcag atgatggaga aatccaaaaa
     4981 gctcgcgtcc cggtccggcg gcggagggcc ttcgggaccg gggccgtcca tgtctccggc
     5041 gttgcgatgg gggttgattg tcctggccgt gttggtcgcc ggttttttgt tcgccgtggc
     5101 gttgaggttg atctgagcgg aaggtcctcg cccgtctgaa agcgaagggc gttccggagt
     5161 ctcctcggag gagagcgcat gagcattgag cagatcgtca tcctggcggc cgtcgcgctg
     5221 gtcgccagcg tgatgctgag caaggtctcc gaacgtttcg gagttcccgc gcttttgctc
     5281 ttcctcggcc tcggcatgct ggccggctcg gacgggcccg ggggcgtcta cttcgacaac
     5341 gcccggctca gccaggcgct gggcaccgtg gccttggcct acatcctctt cgccggcgga
     5401 ctggagaccg acgtccgttc catgcggacg gtgctgaaat ccgccctctc cctgtccacg
     5461 ctgggggtgt tcgtcacggc cctggtcacg ggggcgtgcg cgaaatgggc gttggggctt
     5521 tcctggctgg aaggtttctt gatcggctcc atcgtgtcct cgacggacgc ggcggccgtg
     5581 ttcgcggtgc tccgctccaa gaacgtgggt ctcaaggagc cgctgcggcc gtttcttgaa
     5641 ctggagtccg gcagcaacga tcccatggcc gtgttcctga ccatcgggtt catcgggttg
     5701 atcaaaaacc cggaccggtc gatggcgtcg atggtgccgc tgttcttcca gcagatggtc
     5761 ctgggcttgt tgttcggcta cgtgatgagc aagcttttca tctgggtcat caaccggatc
     5821 aagttggact acgaagggct ctacccggtg ctctgcctgg gatgggtcct tctgatctac
     5881 gggttcacgg ccgccgcggg cggcagcggg ttcctcgccg tgtacatcgc cgggatcatt
     5941 ctggggaatt tcaccttttc gcataaaaag agtctgatgc gtttccagga cgggatcgcg
     6001 tggctcatgc agatcgtcat gtttttcacg ttggggctcc tggtctttcc gaaacagttg
     6061 atcgccgtcg ccggacaggg actcctgatg tccttcctgt tgatcgcggt gggccgtccg
     6121 gtcagcgtgt tcctcgggtt ggcgttgtcg cgcttcaacc tgagagaaaa acttttgatt
     6181 tcgtgggtcg gtctccgcgg ttccgtgccg atcgtcctgg cgacgttccc cctgcaggcc
     6241 ggtctggacc agaaccattt catcttcaac ctggtgttct tcatcgtcat cacctcggtc
     6301 ctcctgcagg ggacgaccat cccgctgttc gcgcgctggc tgggggtgga cgcccctctg
     6361 gtaagaaaac gccgctatcc gatcgatttc gaggattcgg aaaacacgga catgcagctc
     6421 gtggatttca tcgtccccta cggggccaag gcggccggtc aacctattct caagctgggg
     6481 ctgcccaagg acagtctcat cacgctggtc agccggaacg aggagttcat catgccgagc
     6541 ggcgagacgg tgctggagga gggggacgtt ctgctgatgc tcgtcaataa gacctccctg
     6601 ccggcggttc ggcggatatt ggaagcgcgt tagacggtcg tcttcgcggg aaaggaacaa
     6661 aagacacatg gaccattcat ccgtaacgct tctcaccgac atcgcgctcg gcatcatctt
     6721 tgcggcgctg gccgcccacg cgatccggct gttccggcag ccgctgattt tgggatacgt
     6781 cctcggcggc gtcctgttgg ggagccattt cggcttcgga ctggtcacca acgaacagag
     6841 catcgaactt atttccgaga tcggattgat ccttcttctt ttcatcatcg gactggagat
     6901 caacctgcgc gacctggtga agatggggcg gtccatgttc accctgggca tcgtccagtt
     6961 cgcggcgtgt gtcctgctgg ggctcgcttt tttcaggccg atgggccacg ggctggggga
     7021 cgggaatttc gatctgttgt acctggcggt ggcgttggcg ctgagttcga cgctcatcgt
     7081 ggtgaagctg ctgcacgaca agttcgagat ccataccgtt tccggccgtt tgacggtcgg
     7141 cgtgctggtg cttcaggaca tctgggccat cgcgttcatg gcttttcagc cgaacctgtt
     7201 ggatccgcag ctggccagca tcgtcaaatc gttggggctg ggcgccgtcc tgctcctggc
     7261 ctccttcgcc gccagccgcc atctcctgag ccgtcttttc cagtccgcca acaaaagccc
     7321 ggaactcgtc ctcatcacgt ccatggcgtg gtgtttcgcg gtgtccggcc tggccgagcg
     7381 cgccggtctt tccaaagaga tgggggcctt gatcgcgggc atgagcatcg ccgctttccc
     7441 gtacggggcg gacgtcatcg ccaaggtctc cggcgtgcgc gatttcttcg tcacgctgtt
     7501 cttcgtgtcg ctgggcatca aagtgccggc gcccacctgg caggtcgcgg ggacctcttt
     7561 cctggtggcc gggttcgtcg tgttgacgcg gctcctttcc gtcgtgccga cgctctatct
     7621 cctggggagc ggcctgcgca acggtttggt gacggcgttg aacctgtccc agatcagcga
     7681 gttctccctg gtgatcctga gcctgggcgc cggatacgga cacatttccg acggtctttc
     7741 ttccacggtg ttgtcggcca tgctgatgac gtcgattttg gccacctaca tcatcaaata
     7801 caacgatgcc ttgtcccgcc ggctgatgcg ggcgttggcc tgggccggcg ttcgggaaag
     7861 gcacggccac agagaggggt cccttcccga tccttccacc aagaaggaca tcgtcctgct
     7921 gggttgtttt cgggccggga aggcgcttct ggagaccatc gaggaacgcg ctccccattt
     7981 gaaagagcgc gtgctggtgg tggactacaa cgaggcgctg aagggggact tggaatcgcg
     8041 gggcttccag tgggcctacg gcgatctggc ccatccggag acgctccagc atctgggcat
     8101 tgaccgggcc gccttcatca tctgttccct ctcggatacg tttctcaagg ggatcaccaa
     8161 tcggcggctg atgaagcatc tccggcacat ggcgccggcc gcccggctca tcatgtcggc
     8221 ggaagaaaaa cacgaggcgg aaatcctccg gcgcgaaggc gcgcagcacg tcatcgtcgt
     8281 cgggaaactt tccggcatgc atatcttcga aaaactcacg gagaacctgg gcaaacgggc
     8341 gacgtttccc tagaggcggt cgtcggggaa ggggggtggc gttccggcgc gggccgattt
     8401 tcggccggcg ccgttgttat ttcaggggag gacggacggg gagaaggaaa gatcagttcc
     8461 cggatttttc cgggatgggg agggtgaacc agaaacagct gccccggccg acgccgtcgg
     8521 agttgacgcc gatctcgccg ccgtgggcct ggatgatctc cttggcgatg gccagcccca
     8581 ggccgatgcc gtcgcgcttg gaggcgtgcg actccacctg gtagaactgt tcgaagatgc
     8641 ggggcaggtc gccgggcggg atgccctcgc cggagtcgat cacctgcagc cgcacgtcgc
     8701 ttttctgcgt ctccgcccgc accttgacgc tgccgccctc gggggtgtgt ttgaaggcgt
     8761 tggagacgag gttggtgagc acctggtcga tgcggcgctt gtccatgagg agttccggca
     8821 gggcggccgg gtccgcctcc acctgaaaaa cgatcttccg ctgttcggcg agagaggcca
     8881 tgcggttgcc gaccgcctcc accaattcga cgatggatcc ggacgttttt tccacccgca
     8941 gttttcccgc ctcgatgctg acgaaatcca tcaggtcgct gatgagcccg gcgagatggt
     9001 ccgtctcgtg ggcgatgatc ttgaggaagt gctgctgctg ttccgacgga agggtctttt
     9061 gcatggtgag cacctgcgcg tagcccttga tggaggtgag cggagtgcgc aggtcgtgcg
     9121 tgacgatgga gaggaagcgc gacttcaggt cgttgagtcg gttgagctgg tggttggcct
     9181 tctggaggtc cgccaccagg gccttgatct ccagggcgag gcggcgtttt tccagggcct
     9241 tgccgaggga gcgcttgagg tggttggtgt ccacgggttt gatgaggtaa tcgtacacgt
     9301 cggcctggat ggctttcacg gccatgtcca gggaggcgtg ggccgtcatg aggatgacgt
     9361 tgaggtcggg gtcgatctgg cggagttcgc gcgacagggt gaggccggtc atgtcgccca
     9421 gttggaagtc caccagcgcc acgtggtatt ccttgctctt catggcggcg acggcggtcg
     9481 tccccgacgg gtagccgtcc acttcgtagc cgtgctcggt gaggatgtcc accacgaaat
     9541 cgaggatgtt gggttcgtcg tccaccacga ggatccgttc ggtggggggc ctgtcggttt
     9601 cggcccgggc gtgggattcc tggaggcggt cgagcagata cggcaccccg tggggcgcgg
     9661 ggacggccac ccagccgctg gaatgcaccg cggtgcggga ggcctcgtcg agcggctggt
     9721 cgcagaccac cagcaggcgc gcgtcgggac ggtcgtgtcc cgcgtcgcgc aggaacgccc
     9781 cgagggacgt ttccgtgcgg ggtacgcgca ggaccgccag ggccttcgga tttttggcca
     9841 gcgcctcggc caccgccggc agcgtggcgg cggtgacggt gtcggctttc ttgaagaacg
     9901 ggcggatttc ggcgaaattt ttcccggcgg gcgggtcggg cggatcgaag aggatcgtcg
     9961 gctgtttcgg caagagttcc ttcagctgtt ccagcttgtc caccgggtac ggcggataga
    10021 gcacgaacca gcccgcggcg ccgttgagca tatcctgaag cccctcctcc ggcgttttgc
    10081 ccatcaggaa agagaaaaag gccgtgggct ggcggagttt ggtgtcctgg aatatctgcc
    10141 aggtctgttt ttccgtgagg tccatgtcca ggatgtggcc gtggaacggg tagcgttggg
    10201 aaagttgggc gaactcgttg aggcccggcg cgacggtgag cgtcacggcc gaggacacgg
    10261 gggtgtcgta gatcctttcc aggacctcgg cgcggttcga gaggatgagg acgtgtttgg
    10321 gtttggccat gggttcagcg attcttgcgc ggcaagagtt ccgggggccg cgtatagtta
    10381 ctatattcca ctctaaaaac ggacgccgac ttgacggaaa ttgagcaaag aaatataata
    10441 tatttgatac gaaaaaacaa cttattttgg ggtttcgagc cggaagcggc ggctgttttt
    10501 ccggcgtggg cgagtggctc cgccggtgaa cgtttttaag gaggactcgg cgctcgggtc
    10561 ggtggattca tcgacaaggc gcttttccga atgaccatgc ccgcgacctc aacccgcccc
    10621 gccgctccgg tggacacgat gccggaaccg gtttctcccc gcgtcctctt tttttccccg
    10681 gtgcgctccg tctccgatgc ggtggggggg atgttgcagg cggcgggatg tcaggtggct
    10741 ttctgcgatt cggaaagcct cctgccggac tgcctggccg cggggccctg ggacattttc
    10801 ttcggcgacg cgccgcccgc cttcttcccc gcttttttcg acaaggtcgc ctcggttttc
    10861 ccggcggcgc ggggatggct cctcctgccg gacggcgtgg aggcgaagtc cgccgaaaag
    10921 acgttcgaac gttcggaacg gcggtttcgg ctctttgaaa gaccggtgac gctgaacgcc
    10981 ctgttgcggg ccctggagga ggacaccggg gtgggccggg aaggcgcggt gccggacgag
    11041 gcgcgcgtcc tgatcgtgga cgcggacccg gtgttctgcg agaccctgct ggatttcctc
    11101 gccacggagg gctatcgcgg ccgcacggcg cggtccgggc gggaggccat cgacgagttt
    11161 cgccgccagt tctaccacat catcctgctc gattcccgcc gggacgacat gtcggtggag
    11221 gaactggcgc gccggctgca cgaggtgaac ccgcacgcca tggtgatcga gatggcggat
    11281 ttcgcctcgc tcgaccgcgt gctgcacgcc atgcgggccg acgtgcacga ttatctcctg
    11341 aagccgctcg atccgctggc cctccgtcgg accctgcaca acgcgctgga gaaacagaag
    11401 ttggggctgg agatacagtc gctcctgggc cgcctctccc gcgccaaccg cgacctggtc
    11461 cgcgtcaacg agttgaagaa ccagtttttg cgcatcgtga gccacgacct gcgcaccccg
    11521 ctcaccgccg tgaagggata cgcccaggcc ttgaaggccg gcatgatccc accggccaaa
    11581 tacgaacggt gtttcggcac catgtcccgg gagtgcgaaa acctcgaaca cctcatcggc
    11641 gacctggtgg atttcgtcag catcgaggcg gggaagatgc gcctggaaaa ggcgcccacc
    11701 gacgtgcatt cgctgttcga ggggatcgtg gagcggttcc gggacatcat ggaaggaaag
    11761 aagatccgct accgcgtgga gggttttgaa cggacctacc cgccggtgtc ggcggacccg
    11821 cggcggatgg accaggtgct gagcaacctg gtggggaacg ccgccaagca cacgcccgag
    11881 ggcaagagcg tcacggtgca gttccgccgc gccggcgatt tcctcgaggt gcgcgtggtc
    11941 gacacgggcg agggcatctc gccggaacac gccggccggg tgttcgatca gttctatcag
    12001 gccggaccgg ccatggtgca gaaggaaggg ctgggcctgg ggctggccat cgccaaggag
    12061 atcgtgggac gacacggagg gaaaataggg gtgcacagcg acgggttggg cgaagggacg
    12121 acgttctggt tcacgctgcc gttcatgccg gcggcgaagg ggaaatccgc cggagggacg
    12181 ctgtgacggc ccgcatcctc atcgcggagg acgatccgaa cattttgaat ttactctatg
    12241 tttttctgtc gcaacaaggg tatactgtag agaaggccac ggacggacgg gaggccttgc
    12301 gcctggcggc ggcgatcacg ccggatttgc tcatcaccga cgtgatgatg ccccacatga
    12361 acggttatca gctggtgaac gccatcgtct acgagcgggt ggacatcccc acgccgaaga
    12421 tcatcatgct cacctcgcgc accgatccgt ccgacgttca gcggggcctg gacgtggggg
    12481 tggatatgta catccccaaa ccgttcgaca tcgccgaggt gacggcgcgg gtggcggagc
    12541 tgctttccga gggcaaggcg aaaaaggact gacgcatgcg aaaactgaag attttctgga
    12601 tgaaccactg ggtcaagatc gtggtgacgt tcctgatcgt cgccatggtc atcctgacca
    12661 tcctggggct ggcctccctg gagtcgttct atcgcaacct caccctggcc accatcccgc
    12721 tgcaactcct gctcaccgcg ctgaacgccg cggtgttcgt ctatttctac atgtcggtca
    12781 ccaaagggtc cttcggaaaa cttcggaaga acatctcggt gaaggcggag aacgtgcaca
    12841 tctccttcaa ggacgtcatc ggcctggaga acgccaagaa ggaggccatg gaggtggtcc
    12901 agcttctgcg cgaccgcgcc cggctgaagg ccatcggggg acgcatcatc aagggcatcc
    12961 tgctggtggg cccgccgggc accgggaaga ccctcttggc caaggccatc gcctccgagg
    13021 cgggcatccc gttcctgagc atggccggca gcgagttcgt ggaggtgttc gtcggcgtgg
    13081 gggccagccg catccggtcc ctcttcaacc aggcgcgcca gctggcctac gcccacggcg
    13141 cgtccatcgt tttcatcgac gagctcgacg tcatcggccg cggccggcag ttcgacgcgt
    13201 tcggcggcgc gcaggagacc aacagcaccc agaaccagct cctggtggag atggacggtc
    13261 tgggcgaaaa agaggagaac gtcgtggtca tcggcgccac caacgccggc gaggaggtgc
    13321 tggaccgcgc cctcctgcgt ccgggacggt tcgaccgcaa gatctacatc gaccggccca
    13381 acctcgacga gcgcgtggac cttttccgct attacctcaa acgcgtgaaa tacgacccgg
    13441 agatcgacat cggccggctg gcgcgcaagt gcgtgtacaa atcgcccgcc gacatcatga
    13501 acgtcgtcaa ggagagcgcc ctcatcgcca cccgcaacga ccgcaacgtg gtcacctaca
    13561 aggacctctc ccaggccatc gaacgcatcg acctgggcgt ggtgcaccgc ctcaacatga
    13621 ccgcccacga gcgcgaggcg gtggcgttcc acgagaccgg ccacctggtg gtgctctacc
    13681 agctgcatcc gacggacgac gtgttcaagg cctccatcgt ggcccgcggc ggcgccctgg
    13741 gcgtggtgca ccaccagccg cgcgaggaac tgcacacctc cagccgggag aagatcctcg
    13801 ccgacatcaa ggtcgctctc ggcggatacg tcgccgaaaa ggtgcgctac ggcgtcacct
    13861 ccaacggcgt ggcctccgat ttccaggccg ccacccgcat ggcccacgcc atggtctggc
    13921 ggctcggcat gggcaccaac ggctacgtgg gcgatttttc gtccatgccc gaaaacgaga
    13981 tctccgagca cctgaaggag aacctcaaca aggagaccta tcgcctcctc agccagtgcg
    14041 ccaaggacgt ggaggaactc ctgcgcaagg agtgggccct cgtcgaacgt ttcgtcaagg
    14101 agctgctgga acgcgaggag ctggaatacg acgacatcca cgccatcttc accgaatacg
    14161 ggaaggcccg cagtcaggtg cccatccaga ccgtccatcc cgacgacgcc gccccgtcgg
    14221 cgtccgtccc gcctcccgcg gccccggcgg gcccgccccc gccttccgcc tcctgaacaa
    14281 ccgtccgttg cggcgccgtt ccccgctcat cacgtttttg ccgatcggcc gcgctctctt
    14341 gtggccggcg ctcttttctt tgacgggcgt cgcctgcctg aacaggccca cctacaccaa
    14401 ggacgacctg gccgcctccg tccagcgcct ctgtcagaaa gaatacggtt tcaaggcgga
    14461 ggcgcggctg gtcggaaaga cgctgtacgt cgttaccgcg ctggacgggc tggtgggccg
    14521 cgacctgggg ttgcaacgcg agaccatgga aaagctggag ggcgccgtgc tcagctccac
    14581 gcgcgtggcc ctttccacca acgccgacct caattacctc gtggtcaagg cgcgcgattc
    14641 gcgcttgggc gtcaccgtga cgatcctgcg ctatttgccc gacgtgaaga gccttctcta
    14701 tatgcgcatc tcccggtccg atttcatgga ccggcaggtg caggagacgg agaacgccct
    14761 cgagccggaa tcgccggaga gctggcacga cgtgacgatg atggaatttc aggcccggct
    14821 ggtggcctcc cggctccagc ggttgttctc cgagaacccg ctcatcgcgg tgttcctgca
    14881 ggtgcgccgg gtggagggcg tcttcgtcga cggcacgctc tccctgcggc tcgacaagtt
    14941 cgagaacgaa ccggggacga attttttggt ggaggagatc ctccgcaccg ccgtggagga
    15001 cacggtgcgg gacgtggtta aaaagtataa tctgcgcggg atcctcaagc acgtccgcgt
    15061 gtcggacgac gccgaccgcc gcctcctgga cctggaagtg gaggagctga tgagccggcc
    15121 gccgtccccc gcgcccccga agggcgtttt ttcaaaactg cgcgaaaaag aggccgaatg
    15181 aaaaacccga aagaagcgaa atccaagccg gtcgcggccg ggaaagcggc ggaagcggtc
    15241 gccgtccggc agcggctgtt gggggtgttg ctccggaagg gaacctggcc gatgtacggt
    15301 ttcttcaagg aggcgctgca ggggatccag cgcgtggctt tctcgcaggg atacgggttg
    15361 gtcatcgtgg accccgcccc tcagggagag atcagccttc gcgacgccat ccccgcgctc
    15421 cgccgcacgc tggcgggtct gttgatggtg gcgccgccgg aatcggacat ggccgtcctg
    15481 cggccgttga ccgacgagca attccctgtg gtctccctct atcgccggtt cgcggggatg
    15541 aactacgtcg aggtggacaa cgagggcggg gccgaggccg cggtggaaca tctcatccat
    15601 ctgggacaca cgcacatcgc gttcctaagc ggtccggccg acgcggtcga cggccaggaa
    15661 cgcctgtcgg gctaccagaa ggcgctggcc aagaacggat tgaccgtcca taaggaatac
    15721 gttgtggcgg cggatttcga acaggccaag gcctacaagg ccatgaagga cctgctggcc
    15781 ctgaaaaaac cgcccaccgc ggtgttcgcc gccaacgatt atatggcgct cggcgccatc
    15841 tccgccatcc acgacgccgg cctctcggtg cccg
//
LOCUS       DAOTFD010000035        15850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2512_length_15850_cov_4.655587, whole genome shotgun sequence.
ACCESSION   DAOTFD010000035 DAOTFD010000000
VERSION     DAOTFD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_15850_cov_4.655587"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="P5079_03740"
     CDS             complement(<1..1301)
                     /locus_tag="P5079_03740"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HRY29130.1"
                     /translation="MKKQLKSTPKISIKKKGSLLVRQALLVATMDDAGTRLSDADIYI
                     EDGVIRQVGKRLAVKADRVLDARGCVVLPGLVNTHHHLYQTFTRNLPAVQNAKLFDWL
                     IYLYEIWRRLTPEMVHVSALVGLGELALTGCTTSTDHFYVFPKGQPTDLIDEEIAAAR
                     KLGVRFHPCRGSMSRGRSKGGLPPDDVVQSEDAIMKDCDRLVRAYHDPARFSMTRIAL
                     APCSPFSVTTELLKETADYVRRHNLRAHTHLAETIDEEKFCMETQGVRPLGYMEKVGW
                     MGNAFWFAHAVHLNDKEISAMGRTRTGVAHCPTSNLRLGSGIAPLRKMLDKGVPVGLG
                     VDGSASNDSSDILGEARQALLVHRVTSGVGSTTAEEIFRVATRGGAEVLGRDDIGSIE
                     PGKAGDLAIFDLRGLDYAGAVCHDPLAALIFAGISHRTRWTV"
     gene            1664..3067
                     /locus_tag="P5079_03745"
     CDS             1664..3067
                     /locus_tag="P5079_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY29131.1"
                     /translation="MLNFILNQHRVQTDERPGLVLLDFIRDVKGLPGTKEACREGECG
                     ACTVLVGALRNGVLTYKACASCLLPLGDVDGCHVVTVEGLNDDDRLSLAQQLMVDHSA
                     SQCGFCTPGIVLSLTAFGLTSPDYSYADALDALDGNLCRCTGYVAIRNAARTLCDRLA
                     GLDRDPQKRVASLIAAGVLPDYFAGIPQQLAQLPHPAPAPFSPSAVLVAGGTDLFVQK
                     PEALLESDLCFLSKRDDLNYVRLDGRLLRVGGAVTLEEFRRHPEVKKHFPSMEKDLLL
                     HSSTILRNKATLTGNIANASPIGDASILLLALDAKLVLAGPDGARREVPLDKFYRGYK
                     QCDLTCGELIGEILLPLLPPGTRFNFEKVSNRVILDIAAVNTALRLITDPQGRILDLR
                     LAAGGVAPIPLLIGGMERFRGKPVDEATAKAVAQTAADAVKPIDDVRGSAAYKKQLLH
                     QLVLAHFAAAAAPGAAR"
     gene            3064..5307
                     /locus_tag="P5079_03750"
     CDS             3064..5307
                     /locus_tag="P5079_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY29132.1"
                     /translation="MKNSDTSLHVRGASLFIDDIVTPADCLHAALFTSPVARGKIKSL
                     DTRAAARADGVAAVFTHSDVPGLNQVGHVFKDQPLFAVDSVEYIGQPIALVVAATRRA
                     AARAAKLITADIEPLEAVFDPRTAAARGMIHGQKRVITVGDTAAAFAKSKFVAQGKLA
                     TGPQEHFYFETQRCLALPGEHDTLKVCASTQSPGTFHHHIAEVLGIPQNKVEFEIRRL
                     GGGFGGKESTAVWVVAPALAAFLLKRPVKLALGRNEDIATTGKRHAYEYDYKIGLDAD
                     GNILAYEVDLYQHAGACADISLAVLGRSFLHVSGSYRIPNLRATAVSCRTNIPPNNAF
                     RGFGVPQGNFAIEAAIAHAAETMGVDAAIVKRKNLLQDGDTLPYGLQLKTTNAVRCWE
                     TLDKQIDINRRHAAVTEYNRAHDDTKRGLAVIPVCFGISFAQTALNQASSLVNIYVDG
                     SVSVSTGAVEMGQGVNTKIQLIAAQTLGVDADRVRVDTTNTSRIPNASPTSASTGADL
                     NGMATQYACQTLYARLREFAAKHLGHPTPDDIVIKENAVFLKDKRTDLGWDKLVAAAQ
                     WARIDLSHHAFYATPDLHLDPQTERGRPFAYYAYGTSLTEVLLDCRRGTYAIESIDLV
                     HDVGRPLSEAIDRGQVEGAVIQGMGWATIEQIRYAPDGKVLTAVNSYKIPDIKFCPPK
                     FNVTLLPNSDNPFAVCNSKAVGEPPFIHGLGAYFALRHALRSVRQDKEIPSLPLTPEK
                     VFMYLYE"
     gene            complement(5326..6198)
                     /locus_tag="P5079_03755"
     CDS             complement(5326..6198)
                     /locus_tag="P5079_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29133.1"
                     /translation="MEINGMDSLIVKKMIAEYYSIFNTLRQRGLNFKEENPISKVVSA
                     KDKIYEGLKGRSFRKVLRTIYSSQGLSKLELSKKLAISVDALNEILITIKETNAALVR
                     SNPPIYYPKPGAIYEVTFEWFVAELLKRELSGISSFGVKLLNLRYGGDYDVVSRLGDV
                     LLYVECKSGNINNIDKKEILHYFQRDEELSPDLSILLIDSDNMPDSFKQKYAEAFREY
                     VDKKIHILPRIRRNNGMGVFYEGHGTEYIVTAEGNLLSNLRLAVDHYVRFVKRYGLIG
                     PGDEEIKRHYDVYE"
     gene            complement(6779..9112)
                     /locus_tag="P5079_03760"
     CDS             complement(6779..9112)
                     /locus_tag="P5079_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY29134.1"
                     /translation="MKPVEKMKVVHQSVKKIDAPALSRGKHLFTGDLTPPHALHIKML
                     WSPHAHARIKSIDVRAAEKMPGVKCVLYHGNVPRIIHCTAGQGYPEPSPYDTVMFDKK
                     VRYVGDRVAAVAAETPEQALDALDAIKVQYEVLEPVFDYDAALRDGAPVIHDEPDAFV
                     PIPVPYEPKKNIVAKVGMKAGDFEKGMREADFTFDQTFETPYAQHCPIEGYISWAQLD
                     PHGRIVITTSTQVPFHTRRIVARTLDVPVQKIRVIKPRIGGGFGCKQEMLLEDVVAMV
                     ALRTRRPAFWALTRAEVFRSGRTRHPMRLRLRYGVKKNGDISAIGLDIINNTGAYGGH
                     GLTVMSCSGSKVLPLYHVENIHFDAKSIYTNLPVGGAYRGFGATQAYFGVESMVDVMA
                     EKIGMDPLRFRKRNHIVSGESHPAFEALGEGKPGAPMTIGSCSLDECIDKGAREIGWD
                     RRTPPSAKTGRFRRGIGMSIHMQGSSVPEIDMGAASIKMNDDGSFNLHVGATDLGTGS
                     DTILAQIAAEELATTADKFIVYSSDTDMTPFDKGAYASSTTYLSGEAVRKTAAKVKEQ
                     ILKVGAEMLHKNIDEVDVDDAHVVARDGSGKVSYQDIALRSLYEANQFQIAAIESHVT
                     HKSPPPFTAHFAEVEVDMETGKVRVLKYVAAVDCGTAINPQLSEGQTEGAVLNGISYA
                     LTEEYRFDESGKMTNPSFSAYNIFSLRDKPEIKTILVPSYEETGPFGAKSVSEISING
                     PMPSIANAIYNAAGARVYQAPFTQERVWRAIQESKKK"
     gene            complement(9109..>9389)
                     /locus_tag="P5079_03765"
     CDS             complement(9109..>9389)
                     /locus_tag="P5079_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY29135.1"
                     /translation="ALLPGVRLDIVGDGPELHEIQQALIDEGAVQCGYCTPGMVLSAK
                     AMFDENPTPDEETIKVQMDGNLCRCTGYEKIWSAMKKVSASHRNGGKR"
     assembly_gap    9390..9399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9400..9640)
                     /locus_tag="P5079_03770"
     CDS             complement(<9400..9640)
                     /locus_tag="P5079_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY29136.1"
                     /translation="MKMKKISFTLNGVEKEFEIDPNERLLALLRRNGYKGTKYGCLEG
                     ACGACTVIMDGKAVASCLVFAFQAQGRSIVTIEGLG"
     gene            complement(9640..10488)
                     /locus_tag="P5079_03775"
     CDS             complement(9640..10488)
                     /locus_tag="P5079_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY29137.1"
                     /translation="MKIHDFVFATKISEACELLRKTGESAYLIAGGTSTFFVKSRAPR
                     NVIDINRLPLKGIAKKDGVFKIGACTTVNELMKYKAAGWVLNRVALRFVNQQVRNIST
                     IGGNVAKVFYWCDFPVALRVLEGTLTLTGSATHRVKISEVFHNITAQKAAFKNAILEY
                     IEIPQLTKGMGFGYAKETRTSEGFSSVTAAAFVQVEHGLIADVRIALGAVLPFPLRMF
                     DLEASLKGKKADRSAVEGMNFQKLDKYNLTPREGMTPEYCRHLVEVKIADVVSAALAE
                     ATGGSL"
     gene            complement(10533..10775)
                     /locus_tag="P5079_03780"
     CDS             complement(10533..10775)
                     /locus_tag="P5079_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRY29138.1"
                     /translation="MDKIKVTVHFTGVVDVKKIASGESVSLPAGADVSALLSELEIAE
                     RHKKYVVVHVGGRKQGLSHKLKDNDEVKLFLPIGGG"
     gene            complement(10821..12149)
                     /gene="ssnA"
                     /locus_tag="P5079_03785"
     CDS             complement(10821..12149)
                     /gene="ssnA"
                     /locus_tag="P5079_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HRY29139.1"
                     /translation="MSEYLIANGIVVTLGEKNQVILDGAVYVKNGKIGDVGTTKELKA
                     KYKCSVKVNAKNKVVLPGFICAHHHFYSTMARGFCPPGAPALTFQEILERLWWKLDKA
                     LHKDDVYYSALIPIIECIRNGTTTVIDHHASPSYRDGSLDLIADAVTEGGLRASLCYE
                     VSDRNEPGAGIAENERFLKRMRSEKSEFLHGMMGLHASVTVSDDTLAKCRKGAEKYGA
                     GYHIHVAEGVEDHTDSLKKYGVRTVERLVSRGICGKDSLFIHCINVDDKEMDLIKKSE
                     TTVVHNPESNMNNAVGVAKVLKMMSRGILVGLGTDGMASDMPAQMRCSYLVHRLDSRD
                     PRVAFCEAPQMLLWNNAVIANKFFPVKVGVIEKGAAADFAILDYIPPTDMSANNFLGH
                     FIFGMVDAVVDTTIVAGKILMENKKVLALDEEKIAAQSRKLAAKMWTRIS"
     gene            complement(12309..14864)
                     /locus_tag="P5079_03790"
     CDS             complement(12309..14864)
                     /locus_tag="P5079_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY29140.1"
                     /translation="MSNTIQKRKSIPGDTGSSVYDLNLFLTDAQLKAEIEKCEYCEDK
                     PCMGACPCDCSPTDFIKAVSIGEPSDVMRSAALIMGKNPLGGVCGQTCPDKHCMAACV
                     HKKFDSPVSIPAVQATIIQKAKELGVMPKMDKPKSNGKKVAVIGAGAAGLGAASLLAQ
                     RGYGVTLFEKDSVPGGMCNLIPDYRLDKKVLKTDIEWVLRLGDIALKTGETVDAPESL
                     LKKGFDAVVVAAGLWVPLLPGTPNEDKAIAGIPYLKEPGKFDVSGNVAVIGGGATAFD
                     CAMTALVRGAKRVELFALENLSEMPLSAKEMEELVRSGIEVSGRTRVTKILCDGAKVT
                     GLSTVKVRLKDGQKFSLKAIEPVPGSDSTRNDINKVIIAIGARSAFKKGDNPAVFYAG
                     DCVEGPTTVVEASAAGKNTAEQVDAYLNKKPLPTFKRNANGFVKSTAQIPGYNFRPVS
                     LETEFFGRKIKSPFLLSAAPPSDGFDQMKKAYEAGWAGGIMKTSFDNVPIHIPGEYMF
                     QFGDRTYANCDNVSGHSLERVCKEVEKLIKQFPDRMTMASTGGPVSGKDDADRKGWAS
                     NTKKLENAGVMAIEYSLSCPQGGDGTEGDIVSQNAALSQKIITWILESGDGNIPKIFK
                     LTGAVTSMAVIVAAIKEVFDKFPNKKAGITLANTFPTLAFRKGAKKEWEDGIIVGMSG
                     EGALNISYLSLAKAAPLGVEISGNGGPMTYKAAADFLALGCKTVQFCTMPTKMGYRII
                     DDLESGVSHLMAARGIKSMKQLIGVALPNPVADFMALSPVKKISHADHELCVRCGNCK
                     RCPYLAIGFNKDGYPVTDPERCVGCRMCNYLCFVGALGMRERTKEEAAALKEN"
     gene            complement(15216..>15850)
                     /locus_tag="P5079_03795"
     CDS             complement(15216..>15850)
                     /locus_tag="P5079_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY29141.1"
                     /translation="LAVVRDMATTPGRLVNPLNMDAAGQFLTFTLPYVLLSFAYFVYF
                     EGKYGMSLGKRHVGLKVLRENGAALGYYGAFMRTLTFDVFTSSATMKLAAVLQLASGL
                     MVVAGKKRKALHDLVCGTVVVSKKLPRIHRLHNKAILSMAGFSAAAVLANKYAPGFAD
                     KRDELVFLVVALTCLVVARAVKRDAAKIFWYGLFLALAALLFVFVLTRLG"
BASE COUNT         2947 a         5039 c         4658 g         3196 t
ORIGIN      
        1 acggtccagc gcgtgcggtg ggagatgccc gcgaagatca gcgccgccag cgggtcatgg
       61 cacaccgccc cggcgtaatc cagcccgcgc aggtcgaaga tcgccaggtc ccccgccttg
      121 cccggttcga tggagccgat gtcgtcgcgg cccagcacct cggcgccgcc gcgggtggcc
      181 acacggaaga tctcctccgc cgtggtggat cccacgccgc tggtcacgcg gtggaccaac
      241 aacgcctgcc gcgcctcccc caaaatatcg gaactgtcgt tggaggcgga cccgtccacc
      301 cccagcccga ccggcacgcc cttgtccagc atcttccgca acggggcgat gcccgagccg
      361 agccgcaggt tcgacgtggg gcagtgggcc acgccggtcc gcgtccggcc catggccgaa
      421 atctccttgt cgttcaggtg caccgcgtgg gcgaaccaaa acgcgttgcc catccaaccg
      481 accttctcca tgtagcccaa cgggcgaacg ccctgcgtct ccatgcaaaa cttttcctcg
      541 tcgatagttt cggccaggtg cgtgtgcgcg cgcaggttgt gccggcggac gtagtcggcg
      601 gtctccttca ggagttccgt ggtcaccgag aacggcgaac agggcgccag ggcgatgcgg
      661 gtcatggaaa accgggccgg gtcgtgatac gcgcggacca gccggtcgca gtccttcatg
      721 atggcgtctt ccgactgcac cacgtcgtcc ggcggaaggc cgcccttgga ccggccccgg
      781 ctcatggagc cgcggcacgg atggaaccga acccccagct tccgcgccgc cgcgatctcc
      841 tcgtcgatga ggtccgtcgg ctggcctttg gggaatacgt agaaatggtc ggtgctggtg
      901 gtgcagccgg tgagggccaa ctcgcccagc cccaccaacg cgctgacgtg caccatttcg
      961 ggcgtcaacc ggcgccagat ttcataaagg tagatgagcc agtcgaacag cttggcgttc
     1021 tgcaccgccg ggaggttgcg ggtgaacgtc tgatagaggt ggtggtgggt gttgaccagg
     1081 ccgggcaaca ccacgcagcc gcgggcgtcc agcacgcggt ccgctttcac ggcgagccgt
     1141 ttgcccactt ggcggatgac cccgtcctcg atataaatgt cggcgtcgga aagacgggtg
     1201 ccggcgtcgt ccatggtggc gaccaacagc gcctggcgga ccagcaacga gcccttcttt
     1261 tttatggaaa ttttcggcgt ggatttaagc tgttttttca tggtgaacct actgtttttc
     1321 ccggtggatg cgccggagga gtcaacgggg atttttacca tttttcggcc gggttgacca
     1381 ccccggaaat caaacaaaaa acgggaagaa aaaacccgat tttatcggat cttttcttcc
     1441 cgtagtcgtt tcctgcgccg ctggcggaaa aggtagaaac tccctcgtgg gattatacga
     1501 gaagcaacga aatccaatat acctctttga cggaaaaaac tcaagaatga ggctccgccc
     1561 gaggccggcc tccccgccgt tgttgacggt gcccggaaac gaggttacta tgataaaata
     1621 ggggtgtttg caacggccgg aaacccggcg aggggccaag gacatgctga acttcatcct
     1681 caaccagcac cgcgtacaga ccgacgagcg gcccggcctg gtgctgctgg atttcatccg
     1741 cgacgtcaaa ggactgccgg gcaccaagga ggcctgccgc gagggcgaat gcggcgcgtg
     1801 cacggtcctc gtcggcgccc tccggaacgg cgtcctcacc tacaaggcct gtgcctcctg
     1861 cctcctgccc ctcggcgacg tcgacggctg ccacgtggtg accgtggaag ggctgaacga
     1921 cgacgaccgt ctgtcgctgg cgcaacagct catggtcgac cacagcgcct cccagtgcgg
     1981 gttctgcacc cccggcatcg ttctttccct cacggcgttc ggcctgacca gcccggacta
     2041 ttcctacgcg gacgccctgg acgctttgga cggcaacctc tgccgctgca cgggctacgt
     2101 cgccatccgc aacgcggccc ggaccctgtg cgaccggctg gccggactgg accgcgaccc
     2161 ccaaaaacgc gtcgcctccc tgatcgccgc cggcgtgttg cccgactatt tcgccggcat
     2221 cccgcaacaa ctggcgcaac taccccatcc cgcccccgcc cctttctccc cctccgcggt
     2281 cctggtggcg ggcggcaccg acctgttcgt ccagaaaccc gaggccctgc tggaaagcga
     2341 tttatgtttc ctgtcgaaac gggacgacct caactacgtc cggttggacg gccgcctcct
     2401 gcgcgtcggc ggggcggtca ccctcgaaga gttccgccgc catccggaag tgaaaaaaca
     2461 tttcccgtcg atggagaaag acctcctgtt gcattcgtcg accatcctgc gcaacaaggc
     2521 gaccctcacc ggcaacatcg ccaacgcctc gcccatcggg gacgccagca tcctcctgct
     2581 ggcgctggac gcgaaactgg tcctcgccgg accggacggc gcccggcggg aggtccctct
     2641 ggataaattc taccgcggct acaaacaatg cgacctcacc tgcggcgaac tgatcggcga
     2701 gatcctcctc ccgctcctcc cgcccggaac gcgtttcaat ttcgaaaagg tctccaaccg
     2761 cgtcatcctg gacatcgcgg cggtgaacac cgcccttcgg ctgatcaccg acccccaagg
     2821 ccgcatcctc gacctgcgcc tggcggcggg cggggtggcc cccatcccgc tcctcatcgg
     2881 ggggatggag cggttccgcg gaaaacccgt cgacgaggcc acagcgaagg ctgtcgccca
     2941 aaccgcggcg gacgccgtta aacccatcga cgatgtccgc ggatcggcgg cgtacaaaaa
     3001 acagcttttg caccagttgg tgctggcgca tttcgccgcg gcggcggccc cgggagcggc
     3061 ccgatgaaga acagcgacac ctccctgcac gtgcgcggcg cctcgctgtt catcgacgac
     3121 atcgtgacgc ccgccgactg tctccacgcg gcgctgttca catcgcccgt ggcccgcgga
     3181 aagatcaaat ccctcgacac ccgcgccgcg gcccgggcgg acggcgtggc ggcggtgttc
     3241 acccacagcg acgtccccgg cctcaaccag gtgggccacg tcttcaaaga ccagccgctg
     3301 ttcgcggtcg acagcgtcga atacatcggc cagcccatcg ccctggtggt ggccgccacc
     3361 cgccgcgcgg cggcgcgggc ggcgaaactc atcaccgccg acatcgaacc gctggaggcg
     3421 gtgttcgatc cccgcacggc ggcggcccgc ggcatgatcc acggccaaaa acgcgtcatc
     3481 accgtgggag acaccgccgc ggccttcgcc aaatccaaat tcgtcgccca gggaaaactg
     3541 gccaccggcc cgcaggaaca tttctatttc gagacccagc gctgtctggc cctgcccggc
     3601 gaacacgaca ccctcaaggt ctgcgccagc acccagtcgc ccggcacgtt ccaccaccac
     3661 atcgccgagg tcctgggcat cccgcagaac aaggtcgaat ttgaaatccg gcggctcggg
     3721 ggcgggttcg gcggcaaaga atccaccgcg gtgtgggtgg tggcgcccgc gctggcggcg
     3781 ttcctcctca aacggccggt gaaactggcg ctcggccgca acgaggacat cgccaccacc
     3841 ggcaaacgcc acgcctacga atacgactat aagatcggcc tggacgccga cggcaacatc
     3901 ctggcctacg aggtggacct ttatcagcac gccggggcct gcgcggacat ctccctggcc
     3961 gtcctgggac ggtctttcct acacgtctcg ggttcctacc gcatcccgaa cctccgcgcc
     4021 accgcggtga gctgccgcac caacatcccg cccaacaacg cgttccgggg gttcggcgtg
     4081 ccgcagggca acttcgccat cgaggccgcc atcgcccacg ccgcggagac gatgggtgtg
     4141 gacgccgcga tcgtcaaacg caaaaacctc ctgcaagacg gcgacaccct gccttacggc
     4201 ctccagctca agaccaccaa cgccgtccgg tgctgggaaa cgctggacaa acagatcgac
     4261 atcaaccgcc gccacgccgc cgtgaccgaa tacaaccgcg cccacgacga caccaaacgc
     4321 ggattggcgg tcatcccggt gtgtttcggc atctccttcg cgcagaccgc cctcaaccag
     4381 gccagctcgc tggtgaacat ctatgtggac ggcagcgtat cggtgagcac cggcgcggtg
     4441 gagatggggc agggggtgaa caccaagatc cagctcatcg cggcccaaac gctgggcgtc
     4501 gacgccgacc gggtccgggt ggacaccacc aacacctcgc gcatccccaa cgcctcgccc
     4561 acctcggcca gcaccggggc ggacctcaac ggcatggcca cccaatacgc ctgccaaacg
     4621 ctttacgccc gcctgcgcga gttcgccgcc aagcacctcg gccaccccac gcccgacgat
     4681 atcgtcatca aagaaaacgc cgttttcctg aaggacaaac gaaccgacct ggggtgggac
     4741 aaactggtgg cggcggcgca gtgggcgcgc atcgacctgt cccaccacgc tttctacgcc
     4801 accccggacc tccacctcga cccccagacc gagcgagggc ggccgttcgc ctattacgcg
     4861 tatggaacgt cactcacgga agttttgctg gattgccggc gggggaccta tgccatcgaa
     4921 tccattgatt tggtgcacga cgtgggccgg ccgctgagcg aggccatcga ccgcgggcag
     4981 gtggaaggag cggtcatcca ggggatgggg tgggcgacga tcgagcagat ccgctacgcg
     5041 cccgacggga aagttctaac tgccgtcaac tcctacaaaa tacccgacat caaattctgc
     5101 ccgccgaaat tcaacgtcac tctgttaccg aactcggaca accctttcgc ggtctgcaac
     5161 tccaaggccg tgggcgagcc gccgttcatc cacgggctgg gcgcctattt cgccctgcgt
     5221 cacgcccttc gcagcgtgcg ccaggacaaa gaaatcccct ctctccccct cacccctgaa
     5281 aaggtgttca tgtatttata cgagtaaaaa aaactcaaaa ccatattatt catatacgtc
     5341 gtaatgacgt ttgatttcct cgtctcctgg accaattaaa ccatatcgtt ttacaaaccg
     5401 aacataatga tcgactgcaa gtcgtaaatt agaaagaaga ttaccctctg ctgtcactat
     5461 atattctgtt ccatgcccct cataaaaaac acccatccca ttatttcttc gtatcctcgg
     5521 cagaatatga atttttttgt caacgtattc tcgaaaagcc tctgcgtatt tttgtttaaa
     5581 cgaatccggc atattatctg agtcgatgag taaaattgat aaatccggtg aaagttcctc
     5641 gtctctttgg aaataatgaa ggatttcctt tttgtctatg ttatttatgt ttccactttt
     5701 gcattccaca tagaggagca catcccccag gcgagaaaca acatcataat cccctccata
     5761 tcgcaaatta agcaatttca caccgaaact tgaaatccca ctcaactctc gttttaatag
     5821 ttccgcaaca aaccactcaa aagtaacttc atagatcgct cctggttttg ggtaataaat
     5881 tggcggatta cttcttacta gggctgcgtt cgtttctttt atggtaatta atatttcgtt
     5941 caaagcatcc actgaaattg ccaatttctt tgataactcc agtttggaca aaccttgact
     6001 tgaataaatt gttcttagaa cttttcgaaa tgaacggcct tttagtccct cataaatttt
     6061 atccttcgcg ctcactactt tcgaaattgg attttcctcc ttaaaattca atcctctctg
     6121 tcgtaacgta ttaaagattg aataatattc agcaatcatt tttttaacaa ttagcgaatc
     6181 catcccatta atttccatct atatcctctt tcaaaacaaa aaccgtcagt ccatcaactt
     6241 ggactaacgg cccgcaataa aactcaaacc tggattcctg ctctcgcata ctgtgccgca
     6301 gaatatgaca gagcacacaa tgacggcaat gctggcgttt gcctcggttg tttaacccct
     6361 taagggcgct catgagacga agcgattttc ggggccgaag gccaagctgt cctgcggagc
     6421 tgtcaccgcg atgcggtgcc acaggcacca aagctctgcg tggtgcgagc cctccgtggg
     6481 ttattgagga gggcgagttc ttggccggag aaaatcgacg agcgaatgcc cgcaggggcg
     6541 gccttttctt tcccatcttt cttttggccg cgcaaaagaa agatggctgc cgcccgtcag
     6601 tgccatcata caaactggca ccgtatttag cgacacagcc acgggcaaat ttaaagattt
     6661 gttattaaaa acgagattgc ttcggcttcg ttccctaccg acagacaggg accataccta
     6721 gaggcatagc ctcgcaatga cagcgcagga gcacttcaac gccccattaa ccaccactct
     6781 atttcttctt gctctcctgg atggcgcgcc agacgcgttc ctgcgtgaac ggcgcctgat
     6841 acacgcgcgc gccggcggcg ttgtagatgg cgttggcgat ggacggcatg ggaccgttga
     6901 tggagatctc cgaaacgctc ttggccccga aggggcccgt ctcctcgtag ctcggcacga
     6961 gaatggtctt tatctccggc ttgtcgcgca gagagaagat gttgtaggcc gaaaaggacg
     7021 ggttcgtcat ctttccggat tcatcgaacc ggtattcttc cgtgagggca taactgatgc
     7081 cgttcaaaac ggccccctcc gtttgccctt ccgacaactg cgggttgatg gccgtgccgc
     7141 agtcgaccgc ggcgacgtat ttgaggaccc gcaccttgcc ggtctccatg tcgacctcca
     7201 cctcggcgaa atgcgccgtg aacggcggcg gcgatttgtg cgtcacgtgc gactcgatgg
     7261 cggcgatctg gaactggttg gcctcgtaca gcgaccgcag ggcgatgtcc tgataggaaa
     7321 ccttgcccga accgtcccgc gccaccacgt gggcgtcgtc cacgtccacc tcgtcgatat
     7381 ttttgtgcag catctccgcg cccaccttga ggatctgctc cttcaccttg gcggcggtct
     7441 tgcggacggc ctcgcccgag agataggtgg tgctggaggc gtaggcccct ttgtcgaagg
     7501 gggtcatgtc cgtgtcggat gaatacacga tgaacttgtc ggcggtggtc gccagctctt
     7561 cggcggcgat ctgcgccagg atggtgtcgc tgccggtgcc caggtcggtg gcgccgacgt
     7621 gcaggttgaa cgacccgtcg tcgttcattt tgatggaagc cgcccccatg tcgatctcgg
     7681 ggacgctgga cccctgcatg tggatgctca taccgatgcc ccgccggaac cggccggtct
     7741 tggcggaggg cggggtgcgc cggtcccacc cgatctcccg ggcgcctttg tcgatgcatt
     7801 cgtccagcga gcagctgccg atggtcatgg gcgcgccggg ttttccttca cccagcgctt
     7861 cgaacgccgg gtggctctcg ccggagacga tgtggttccg tttccggaac cggagcgggt
     7921 ccatgccgat cttttcggcc atgacgtcga ccatcgactc cacgccgaaa taggcctgcg
     7981 tggcgccgaa cccgcggtag gcgccgccca ccggcaggtt ggtgtagatg gatttggcgt
     8041 cgaaatggat gttctccacg tggtagagcg gcaacacctt gctgccggaa cagctcatga
     8101 cggtcagccc gtgcccgccg taggcgccgg tgttgttgat gatatccagc ccgatggccg
     8161 agatatcgcc gttctttttg accccgtacc tcagccggag gcgcatggga tgccgcgtcc
     8221 ggccgctgcg gaaaacctcc gcgcgcgtca gcgcccagaa ggcgggacgc cgcgtcctta
     8281 aagcgaccat ggccaccacg tcttccagca gcatctcctg cttgcacccg aacccgccgc
     8341 cgatgcgcgg tttgatgacc cgtatcttct gcacgggaac gtccagcgtg cgcgccacga
     8401 tccgccgcgt gtggaacggg acctgcgtgc tggtggtgat gacgatccgc ccgtgcggat
     8461 cgagctgcgc ccaggagatg tatccctcga tggggcaatg ctgggcgtag ggggtttcaa
     8521 aggtctggtc gaacgtgaaa tccgcctcgc gcatcccctt ctcgaaatcg ccggctttca
     8581 tgccgacctt ggccacgatg ttcttcttgg gctcgtaggg gacggggatg gggacgaagg
     8641 cgtcgggctc gtcgtggatg acgggcgcgc cgtcgcgcag ggccgcgtcg taatcgaaca
     8701 ccggctccag cacctcgtat tggaccttga tggcgtccag ggcgtcgagc gcctgttccg
     8761 gcgtctccgc cgccaccgcc gccacccggt cgcccacgta gcggaccttc ttgtcgaaca
     8821 tgacggtgtc gtagggggac ggttcgggat aaccctgccc cgcggtgcaa tggatgatgc
     8881 gcggcacgtt gccgtgataa aggacgcatt tcacgcccgg catcttctcc gcggcgcgga
     8941 cgtcgatgga ctttatgcgc gcgtgggcgt gcgggctcca gagcatcttg atgtggagcg
     9001 cgtggggcgg ggtgaggtcg cccgtgaaca gatgtttccc ccgggacaac gccggggcgt
     9061 cgatcttctt gaccgactga tgcaccacct tcatcttttc gaccggtttc atcttttccc
     9121 gccgttccta tggcttgcgg acaccttctt catggccgac catatctttt cataaccggt
     9181 gcaccggcac aggttgccgt ccatctgcac cttgatggtc tcctcgtcgg gcgtggggtt
     9241 ctcgtcgaac atggccttgg cggagagaac catgccgggg gtgcaatacc cgcactggac
     9301 cgccccttcg tcgatgaggg cctgctggat ctcgtgcagt tcggggccgt cgcccacaat
     9361 atccagtctc acgcccggca gcagcgccan nnnnnnnnnc gcccagcccc tcgatggtga
     9421 cgatgctgcg gccctgggcc tggaacgcga acaccagaca ggacgccacg gccttgccgt
     9481 ccatgatgac ggtgcaggcg ccgcaggcgc cctccagaca gccgtatttg gtgcccttgt
     9541 atccgttgcg gcgcaggagg gcgagcaacc gctcgttggg gtcgatctcg aactcctttt
     9601 cgacgccgtt cagcgtgaac gatatttttt tcattttcat catagggacc cgcccgtcgc
     9661 ctcggccagc gccgccgata cgacatcggc gatcttgact tccaccagat gccggcagta
     9721 ttcgggggtc atcccctcac gaggcgtcag attgtatttg tccagcttct gaaaattcat
     9781 cccttccacg gccgagcggt ccgccttctt ccccttcagg gaggcctcga ggtcgaacat
     9841 ccgcaggggg aacggaagca ccgcccccag ggcgatgcgg acgtcggcga tgagcccgtg
     9901 ttccacctgg acgaacgccg ccgccgtcac ggaactgaac ccttcggagg tccgggtttc
     9961 tttggcgtaa ccgaacccca tgcccttcgt caattgcggg atttcgatgt attccaggat
    10021 ggcgttcttg aacgcggcct tctgcgccgt gatgttgtgg aacacctcgg agatcttcac
    10081 ccggtgcgtc gcggaaccgg tgagcgtcag ggtcccctcc agcacgcgca gggccaccgg
    10141 gaaatcgcac cagtagaaca ccttggcgac gttgccgccg atggtggaga tgttccggac
    10201 ctgctggttg acgaaccgca gcgccacccg gttcagcacc cagccggcgg ccttgtattt
    10261 catgagctcg ttgacggtgg tgcaggcgcc gatcttaaag acgccgtcct ttttcgcgat
    10321 gcccttcagc ggaagacggt tgatgtcgat gacgttccgc ggggcccgcg acttcacgaa
    10381 aaacgtggag gtgccgccgg cgatgagata ggccgactcc cccgtcttcc tcagaagctc
    10441 gcaggcctcc gatatcttgg tggcaaaaac aaagtcatgt attttcattc tctgctcccg
    10501 ctaaaaccgc ttttggattc aaaacgtccg cttcaaccgc caccgatggg caaaaacagc
    10561 ttgacctcgt cgttgtcctt cagcttatgg gaaaggccct gttttctccc tcccacgtga
    10621 accaccacgt atttcttatg ccgctcggcg atctcgagct ccgacaggag cgcgctcacg
    10681 tccgcccccg ccgggagcga cacggactcc ccgctggcga ttttcttcac gtccaccacg
    10741 ccggtgaaat ggaccgtgac cttgatctta tccatcgcgt cgccccgccg cccgtcgaga
    10801 gggagtgtcc ggccggggcg ctagctgatc ctcgtccaca tcttggcggc gagcttccgg
    10861 gactgcgcgg cgatcttctc ctcgtccagc gccagaacct tcttgttctc catgaggatc
    10921 ttccccgcca cgatggtggt gtccaccacg gcgtccacca tgccgaagat gaaatggccg
    10981 agaaagttgt tcgccgacat gtcggtgggc gggatgtagt ccaggatggc gaaatcggcg
    11041 gcggcgcctt tctcgatcac gcccactttc accgggaaga acttgttggc gatgacggcg
    11101 ttgttccaca ggagcatctg cggggcctcg cagaacgcca cgcgcgggtc gcggctgtcc
    11161 aggcggtgca ccaggtagga gcagcgcatc tgcgcgggca tgtccgaggc catgccgtcg
    11221 gtgccgagcc ccaccaggat cccccggctc atcatcttca acaccttggc cacgcccacg
    11281 gcgttgttca tgttggattc ggggttatgc accacggtgg tctcgctctt cttgatgagg
    11341 tccatctcct tgtcgtccac attgatgcag tggatgaaga gggaatcctt cccgcagatg
    11401 ccgcggctga cgagccgctc cacggtccgc acgccgtatt tcttcaggga atcggtgtgg
    11461 tcctcgacgc cttcggcgac gtggatgtgg tatcccgcgc cgtatttctc ggcgcccttg
    11521 cggcatttgg ccaaggtgtc gtccgacacc gtgacggagg catgaagccc catcatgccg
    11581 tgcagaaact ccgacttctc cgaacgcatg cgtttcagga accgctcgtt ctcggcgatg
    11641 ccggcgccgg gctcgttgcg gtcggacacc tcatagcaca ggctcgcgcg gaggccgccc
    11701 tcggtcaccg cgtcggcgat caggtccagg ctgccgtcgc gataggacgg gctggcgtgg
    11761 tggtcgatga cggtggtggt gccgttgcgg atgcactcga tgatggggat gagggcgctg
    11821 taatacacgt cgtccttgtg gagcgccttg tcgagtttcc accagaggcg ctcaaggatc
    11881 tcctggaagg tgagggcggg ggcgcccggc gggcagaacc cgcgggccat ggtgctgtag
    11941 aaatggtgat gggcgcagat gaacccggga aggaccacct tgttcttggc gttcaccttc
    12001 acggagcatt tgtatttcgc cttcagttcc ttggtggtgc ccacgtcgcc gatctttccg
    12061 tttttgacgt agacggcgcc gtcgaggatg acctggttct tttcccccag ggtgacgacg
    12121 atgccgttgg ctatcagata ttcgctcatg tttggtctcc gttgttgaga tatcgtgttt
    12181 tcgatgaaac tttcaaacct tgtcgtacgg cgacattccg gcccgccgac gaaagatcac
    12241 ttttaagcgg cgagatcgct tcggtcaaaa acggacctcg caatgacgcc gaggggaaca
    12301 accggccgtc aattttcctt cagcgccgcg gcttcctctt tggtgcgttc cctcatgccg
    12361 agggcgccca cgaaacacag atagttgcac atccggcagc cgacgcaccg ttccggatcg
    12421 gtgacggggt atccgtcctt gttgaaaccg atggccaggt acggacaccg tttacagttg
    12481 ccgcaccgca cgcagagttc atggtcggcg tgggagatct tcttgaccgg cgacagggcc
    12541 atgaagtcgg cgacggggtt cgggagcgcg acgccgatga gctgtttcat ggacttgatg
    12601 ccgcgcgcgg ccatgagatg gctgacgccg gactccaggt cgtcaatgat gcggtaaccc
    12661 atcttggtgg gcatggtgca gaactgcacc gttttgcagc cgagcgccag gaaatcggcc
    12721 gcggccttgt acgtcatggg cccgccgttc ccggagattt ccacgcccag gggcgcggcc
    12781 ttggccaacg agaggtagct gatgttgagc gcgccctcgc cggacatgcc cacgatgatg
    12841 ccgtcctccc actccttctt ggcgcctttt cggaacgcca gcgtggggaa ggtattggcc
    12901 agggtgatgc ccgccttttt gttggggaac ttgtcgaaca cctccttgat ggcggccacg
    12961 atgaccgcca tggaggtgac ggcgcccgtc agcttgaaga tcttggggat gttgccgtcg
    13021 ccggattcga ggatccaggt gatgatcttc tgggacagcg cggcgttctg ggagacgatg
    13081 tcgccctcgg tgccgtcgcc gccctgcggg caggagaggc tgtactcgat ggccatgacg
    13141 cccgcgtttt ccagtttctt tgtgttgctg gcccagccct tccggtcggc gtcgtccttg
    13201 ccggaaaccg ggccgccggt ggaagccatg gtcatgcggt cggggaactg cttgatgagc
    13261 ttttccacct ctttgcagac ccgttccagc gagtgcccgg aaacgttgtc gcagttggcg
    13321 taggtgcggt cgccgaactg gaacatgtat tcgccgggga tatggatggg cacgttgtcg
    13381 aaggaggtct tcatgatgcc gccggcccag cccgcctcgt aggccttctt catctggtcg
    13441 aacccgtcgc tgggcggcgc cgccgacagc aggaacgggg attttatctt ccgtccgaag
    13501 aactccgtct ccagggacac cgggcggaaa ttgtagcccg gaatctgggc ggtgctcttg
    13561 acgaacccgt tggcgttcct cttaaaggtg gggaggggct tcttgttgag gtaagcgtcc
    13621 acctgttcgg cggtattttt gccggccgcg gacgcctcca ccacggtggt gggcccttcg
    13681 acgcagtcgc cggcgtagaa cacggcgggg ttgtcgccct tcttgaacgc ggaccgcgcg
    13741 ccgatggcga tgatgacctt gttgatgtcg ttccgggtgg agtcgctgcc cggcaccggt
    13801 tcgatggcct tcaggctgaa cttctggccg tccttcagtc tcaccttcac cgtggacaac
    13861 cccgtcacct tggcgccgtc gcacaggatc ttggtgaccc gggtgcggcc gctcacctcg
    13921 atgccgctcc ggacgagttc ctccatctcc ttggcggaga ggggcatctc gctcaaattt
    13981 tccagcgcga acagttccac ccgtttggcg ccgcggacca gcgcggtcat ggcgcagtcg
    14041 aacgcggtgg cgccgccgcc gatgacggcc acgttgccgg acacgtcgaa tttcccgggc
    14101 tctttcaggt aggggatgcc ggcgatggcc ttatcctcgt tgggcgtgcc gggcaggagc
    14161 ggcacccaga gtcccgcggc caccaccacg gcgtcgaacc cttttttcag gagcgactcg
    14221 ggggcgtcca cggtctcccc cgttttgagg gcgatgtcgc cgagcctgag gacccactcg
    14281 atgtcggtct tcaacacttt cttatccagg cgataatccg gaatcaggtt gcacatcccc
    14341 cccgggacgg agtccttctc gaaaagggtg acgccgtagc cgcgttgggc caggagggag
    14401 gcggccccga ggccggccgc gcccgcgccg atgacggcga ctttcttgcc gttcgatttc
    14461 ggtttgtcca tcttgggcat cacgcccagt tctttcgcct tctggatgat ggtggcctga
    14521 acggcgggaa tgctgaccgg ggaatcgaac ttcttatgga cgcaggcggc catgcagtgc
    14581 ttgtccggac aggtctgtcc gcagaccccg cccagggggt tcttgcccat gatgagggcg
    14641 gcggaacgca tcacgtccga cggttcgccg atggacaccg ccttgatgaa atccgtgggg
    14701 gagcagtcgc aggggcaggc ccccatgcac ggcttgtcct cgcagtattc gcatttttcg
    14761 atctccgcct tcagttgggc gtcggtgagg aaaaggttca ggtcgtaaac ggagctgccc
    14821 gtgtcgcccg ggatggattt tcttttctgg atcgtgttgc tcatgatggc tctcctctgt
    14881 ggtctcgaat tattaaagcg ttttaccgct catccgggtc tccgatgcgg gaggacggat
    14941 ttttatgacc tttatttttg acgcgacgtg gaggcgtcgg acagcttaca aaaaatcccg
    15001 tcggtccgaa atcaaaaaac ggccgccggt ataacaaaaa agggaagaaa aaacccatcg
    15061 tgtcgccagg ttttttcttc ccgtagcgcg cccgtctttc tcaaccgacc gggccgtaga
    15121 aactccctcc agggatatac gagaagccgt tgaacgtctt caggaagtat acttctgcgt
    15181 ttttaaaaaa tcaataggcc gcggaaaagc ccggatcagc ccagccgcgt cagaacaaac
    15241 acaaagagga gagccgcgag ggccaggaaa agcccgtacc agaaaatttt agcggcatct
    15301 cttttcaccg cccgcgcaac caccaaacat gtcaacgcga cgaccaagaa gaccagttcg
    15361 tccctcttgt cggcgaagcc gggcgcgtat ttgttcgcca aaaccgccgc cgccgaaaaa
    15421 cccgccatcg acaggatggc cttgttgtgc aggcggtgta tccgcggcaa ttttttcgac
    15481 accaccacgg tcccgcagac caaatcgtgc agggctttcc tttttttacc cgccacgacc
    15541 atgaggccgc tcgccaactg aaggacggcg gcgagtttca tcgtcgcgga cgacgtgaaa
    15601 acgtcaaagg tcagcgtcct catgaaagcg ccgtaataac ccagcgcggc gccgttttcc
    15661 cgaagcacct tcaatccgac gtgccgtttc cccagactca tcccgtattt gccctcgaaa
    15721 taaacgaagt aggcgaaact gagaaggacg tacggcaggg tgaaggtgag gaattggccg
    15781 gcggcgtcca tgttcaacgg attgaccaga cggcccggcg tcgtcgccat gtcccgcacg
    15841 acggccagaa
//
LOCUS       DAOTFD010000036        15615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2567_length_15615_cov_4.224743, whole genome shotgun sequence.
ACCESSION   DAOTFD010000036 DAOTFD010000000
VERSION     DAOTFD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_15615_cov_4.224743"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5079_03800"
     CDS             complement(<1..510)
                     /locus_tag="P5079_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRY29142.1"
                     /translation="MSVPAAARKEAERLRREIARHEHLYYAEAKPVISDYDFDLLLKK
                     LEALEKQYPDLRTLDSPTQRVGGKPVEGFPTHRHAVPMLSLDNTYSTDDLNDWHGRVL
                     KGLRPGDPLEYVVELKIDGVGLALTYEDGLLTTAATRGDGETGEDVTANARTIRSIPM
                     RLHGKAPKLL"
     gene            complement(515..952)
                     /gene="nusB"
                     /locus_tag="P5079_03805"
     CDS             complement(515..952)
                     /gene="nusB"
                     /locus_tag="P5079_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY29143.1"
                     /translation="MGARRQAREAALQILYLVDVGKFDLEKAAAACWTESTLAPRART
                     FADDLARGALENMPLLDGLITKYAENWELSRMAAVDRNILRLTAYELLFHVDTPVSVA
                     IDEAVEIAKTYSTQDSGKFVNGILDKIKLERGKTAPPTPAAKE"
     gene            complement(954..1448)
                     /gene="ribH"
                     /locus_tag="P5079_03810"
     CDS             complement(954..1448)
                     /gene="ribH"
                     /locus_tag="P5079_03810"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRY29144.1"
                     /translation="MKIPSHQKRPGAAPADGRGLRIAVVAARFNAPVTERLLDGCRRG
                     LAECGVRARDISVTWMPGAFELPLAAQTLAQSKKYDAVITLGCILRGETPHDRYIAQA
                     TAGGVAQAALATGVPVMFGVLTPLSVAQARARAGTGTDNKGYEVAVAAVETARLLRPL
                     RRGK"
     gene            complement(1445..2662)
                     /locus_tag="P5079_03815"
     CDS             complement(1445..2662)
                     /locus_tag="P5079_03815"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRY29145.1"
                     /translation="MSPSAKKTFVSVPEALRDFERGKPIVVVDDPGRENEGDLVIAAE
                     KATPAAVNFMAKHGRGLICVPMLGERLDELKISPMVENGSSREAAFTVSVDARRGVTT
                     GISAHDRSHTIKTLIHSKSRPEDISKPGHIFPLRYKEGGVLVRSGHTEAAIDLARLAG
                     LYPAAVICEIMNEDGTMARLPRLKRFARKYRLKMMTIQDLIAHRRRHEKLVRRVVSAK
                     LPTRLGDFQIHLYEDIPTGDHHVAVVKGEVAGRPNVLVRVHSSCFTGDVLHSLRCDCG
                     DQLQSALQQIERARRGVLLYMHQEGRGIGLVNKLHAYVLQEIGYDTVQANKKLGFPAD
                     LREYGIGAQILADLGLSTVHLLTNNPHKIVGIEGYGLKVTKRLSLEIPPNPMNRRYLE
                     TKRRKLGHLLKHL"
     gene            complement(2659..3309)
                     /locus_tag="P5079_03820"
     CDS             complement(2659..3309)
                     /locus_tag="P5079_03820"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRY29146.1"
                     /translation="MFTGIIQTLGDVVSRNGEKLRVRARLPRLKKGASVAVDGVCLTV
                     VSFTGKKPPQVLTFDVSTETYQRTTLGRYRPGQKVNLETPLRAGDEFGGHFVLGHVDG
                     TGKLVSKTPAGPATVLYEFSVPPHLMDHLAPKGSVAVDGISLTVVDIKEVSFTVAIIP
                     HTEANTSLGAKKPGDPVNLETDMLAKHLDRLLAARRETPTDDLFVKKSLIPEEEYQ"
     gene            complement(3311..4399)
                     /gene="ribD"
                     /locus_tag="P5079_03825"
     CDS             complement(3311..4399)
                     /gene="ribD"
                     /locus_tag="P5079_03825"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRY29147.1"
                     /translation="MSLKDDEFYMRRALALAEEGRGRTHPNPMVGCLIVKNGAVVGEG
                     WHGSFGGPHAEAVALRRAGKRAQGATLYVTLEPCRHWGQTPPCSDAVQRAGIKRVVAA
                     MKDPNPVSGNGLDRLRRAGIRVETGVLRNEARFLNRAFVKRHETGLPYVLFKAGQTLD
                     GKIASRSGVSRWITGPLARELGHRLRAESDAILVGAHTVRKDNPRLTSHGKGPDPVRL
                     VVSKSLDLPVSAKIFKGGAPTWVITGPTPPAGRKKRLEKAGIQLLTCPLKNGDINLML
                     ILKLLAKMNINQILLEGGGRLSGALLDARLLDEVYLFLAPRLLGGRHAVPSVAGGGWP
                     HPNSAPRLDNMTVSKVGGDILVHGFVKY"
     gene            complement(4547..6058)
                     /locus_tag="P5079_03830"
     CDS             complement(4547..6058)
                     /locus_tag="P5079_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRY29148.1"
                     /translation="MTRSLLHGPKSLFHLILVSLLAAAETLGSPRPAFAADAQDALKK
                     SVVKIYTVSKEPDYTEPWRTKEQEQGSGSGCILPGNRILTNAHVVGNQVFLQVSKPGD
                     PERYEAKVEFVAHDGELALLSVDDPVFFKNTRPVSFGDLPRQRDKLAAYGFPVGGEEL
                     SITEGIVSRIEVQRYQHSRRELLAIQTDAAINPGNSGGPVFKEGKLAGISFQSLKGGR
                     TENIGYAIPTPIIQRFLKDVDDGDYDGVPSLGIFWQKLENATFRKYCGLAAKESGILV
                     TKIPYGAAAVNAFQVGDVLLSIDGQPVANDGTVSLRGDERVEFSHLISTRQIGEPVQL
                     KILRDGRRLDATLALGKHVTLVSGPQYDTVPTYFIFGGLVFMPLSHNYIEERGMRSVL
                     PAVLDYLFTDLPTKERRQVVLISKTLPHNINVGYHYWLRDAIVKSVNNVPVAEMRDVL
                     QGLQKPEGPYHVIELESPPYASRIILDAAQAEKATREILERFQIPADRSEDLK"
     gene            complement(6130..6864)
                     /locus_tag="P5079_03835"
     CDS             complement(6130..6864)
                     /locus_tag="P5079_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY29149.1"
                     /translation="MKYGVLADIHSNEEALRAVLTLLREQGVKGFLVVGDIVGYGPNP
                     DETVAAVRALPHGRAVAGNHDRAVVGKKDLDGFNPHARAAALWTARQLTPASKKFLSS
                     LPDRVATDRFTLVHGTPQNPIDDYFTTSDQFRANEGLFETPVCFVGHTHVPRVLRRHG
                     DGAKVSAWNNGETIKTPPRDKTVLNPGSVGQPRDGDPRAACGVYDDETGEATLFRVAY
                     DVAAVQKKMRDARLPAFLADRLAEGR"
     gene            complement(6869..7621)
                     /locus_tag="P5079_03840"
     CDS             complement(6869..7621)
                     /locus_tag="P5079_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRY29150.1"
                     /translation="MTGLSPMPPDSPRTSVLGFGIDSLDLPALLSRVESFLSSGAPRQ
                     LITANALMLLAARENPALAEAFRAADLVVPDSAGVAMAGRWRGRPFPATIPGVELIEH
                     LCRRAAETGRSVFFLGAAPGVAEKTAERLKEKYPALRVAGTENGYFRRETESLAILAV
                     ARAAPDLLFVALDVPRQEIWIRQNLGRLKCKIAMGVGGSFDVLSGRLKRAPRWMRIAG
                     LEWFFRLLQEPSRAVRMSRLPEFFFRVLLDRP"
     gene            complement(7655..11185)
                     /locus_tag="P5079_03845"
     CDS             complement(7655..11185)
                     /locus_tag="P5079_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY29151.1"
                     /translation="MYLKRMEMVGFKSFADRTRLDLEPGITAVIGPNGCGKSNVVDAI
                     RWCLGEMSAKSLRSKILLDVVFNGSANRAPSNMAEVSLTFDNSQNRLPIDYSEVTVTR
                     RLFRSGESEYFMNKTQCRLKDIRELFLDTGIGEEGYSIMEQGRVEYVLSARPEERREL
                     FEEAAGVSKYKARREEALRKMERTQLDLDRLADVIAMTKEQMDKIEAAVRKARLYQKA
                     QEDLKVMEISHALWETSQLDQQITALKQEIQRMEEDLQSKTTLINQREAELAELRLAE
                     TQLGERMVAQNREISDVDGAIGLAEQKQADARRWEESIRQRDTVLDEELAKGRVRLEE
                     LEKACAEFQQALDAEAKTAEEAGLALKKEEESYAVLAERQKTTAGEMKGLQDALWKNN
                     QDATQLHNEIGAKRSLETRLDTELKTLQKDRAKSEEKLQAAQTALTQYDQDIQARDAE
                     LRRLEETVAQARAALAQTEADLTALAKSSEEVQSKLFQCKAQLEAQEKWAADDVFSQG
                     AHAVLSAGLSGLHGPLGKLIAVEPADEPALRQVMGPHLNDLVADSLHEAQAALQHVTD
                     HRQGRVRVWVLDRLPQGDVSAGLSGGGQRSLLEMVRADAKFQPVLKFLLSRWLAAGTT
                     LYGEGVIEGGAEFAPVRGEDPLLRASLEAESPRLANELELALAKRTALEDQKRLHMDE
                     WEKAHQATEAARVQKNFFAQEHEKTRIQTGLFQDEIRLIDGESERLQNERRQAEETAR
                     ALAEKLRELTLEEARLREQWTLLQERTQKEQIELSDAGALLGAVRERAQSQQERLRWR
                     EKQLHAARSDQDALAAGLESKQQERSTSQQRIEEQQKIQEECRQQIETSMAKRKELEQ
                     ALEGIHAEKQTLYDRLTASQNELTTLRDSSEEIRTQLQDKKLQANHSEFKRESFETQL
                     KDKYGLTLLEAQERHPLPTQAVVLTDLERLRRRVESLGAVNLAAPEEHAELEQRYNFL
                     LSQQQDLLKAKEDLLQTIQKINATTRSSFRDCFDKVRENFKAIYGQLFQGGEADLRLT
                     DESDILNTGIEVFAQPPGKKLQNIALLSGGEKALTAVALLFAFFMVRPAPFCILDEVD
                     APLDEANVTRFITLIKAFTHQSQFLMITHNKRTMETADVLYGVTMETLGVTKLLSAKL
                     RKDGRLAETLSPEAVIAGDSRQEPETKPAE"
     gene            11437..12447
                     /gene="waaF"
                     /locus_tag="P5079_03850"
     CDS             11437..12447
                     /gene="waaF"
                     /locus_tag="P5079_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRY29152.1"
                     /translation="MKRKIAVRAPNWLGDVVMCTPFLKRLIEKNPEAEVHVLCRPAHG
                     EIFSRFPGVKGVRLLGKDEPWAALVRRVAAEKFQTGYVLPPSFSSALLFFMAGVPERI
                     GYATDFRRFFLTRALALDERFHYVRRYLGLLGEESREAATSDLFFPAPPDAAHGARAF
                     LLARGFSFKTPCLAVAPGSQAPGRRWEPERFAAVIGALAEKDWPAVVLVGAPGDLPWA
                     EKVRHACSRPVINLCGATDLVTLGDLLRQCSALLTNESGMMHVAWAVGTPTVALAGPS
                     EPRVTSPFGAPVRVLQHREIPCVPCIRNDCYLAGDDYKLCLKSIAVSEVVSALNEVVK
                     TS"
     gene            complement(12529..13806)
                     /locus_tag="P5079_03855"
     CDS             complement(12529..13806)
                     /locus_tag="P5079_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY29153.1"
                     /translation="MPNSIFVTKERCTGCTACVKACPVSCITMVDRDPNEGVKWRKLA
                     VIDEVKCVFCNACVEVCDNLGAKSAHKDLFNAITMTKETATSTGPAIDTSLYKGVWVY
                     AEVRHGKLVPTIYELLSLAQKLAGTLGEDISAVLIGKDVARHAQDIIDHGADKVYVLD
                     HPCFENFVDELYTQAFADLILKEKPNKLLLPASTLGRSFASRLAISAKTGITADATEL
                     TIDPKTRLLHATRPSFGGNLMATILCEKQRPEMATVRPMSFPRSEKKPGRQGKTIPVP
                     VDPSKFKQRTRFVKYEPEQGEEQDISAADVIVAGGRGVGGAAGFKPIEALAKILGGAV
                     GASRAAVDAGWITYRHQVGLTGRTVRPKLYIAAGISGQIQHLAGMNSSDVIVCVNKDP
                     EAPLMKLATLSIEADLFEFIPALVKELEKRKGH"
     gene            complement(13809..14618)
                     /locus_tag="P5079_03860"
     CDS             complement(13809..14618)
                     /locus_tag="P5079_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRY29154.1"
                     /translation="MGLHIVVCIKQTPATSNIQIDPATGTLKREGMAAAINPFDEYAI
                     EEAVRIKERVPGTTVSVITMGPPQAEDSLRDAIARGCDQAFHITSRAFAGADTWATSY
                     TLHMGIKKIAKEKGPVHLVICGKQTNDGDTGHVGPGIGAWLDWPNVAYIKKVDQVDDK
                     KIVVHRMMEDGTDILEMDLPAVIAVVKEINTPRVASLKGKMAAKKAAIPKWDEVALEC
                     DKSNIGLAGSPTVVSKSFNPPPRQGGVRLDGATVDEKAKKLVDKLQELKLI"
     gene            complement(14736..>15615)
                     /locus_tag="P5079_03865"
     CDS             complement(14736..>15615)
                     /locus_tag="P5079_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY29155.1"
                     /translation="AVVFWAVASEPASGSDATAQKTIAKKDGDSYVLNGTKCFISNGE
                     VAETYTVFASTNPSRGPRGISCFIIEKGTPGLSFGKKETKMGIRSSATYEVVLNNCRV
                     PAQNLLAKEGHGLFVAQATFDMSRPGVASQALGIGTGAFNEALAYAKMRQQFGQSVLS
                     FQSSQHLVADMATRLETGRALLYSVTRAMDIDFKAAVEASEANGTLVYDELKKVAKQR
                     LTKYSAMCKLYCSDTADWVTGKCIDLCGGIGYMQDFPIEKFHRDAKITQIYEGTNQIQ
                     RNEIGAMITKELASGK"
BASE COUNT         2366 a         4679 c         5356 g         3214 t
ORIGIN      
        1 caggagtttc ggcgcttttc cgtgcaggcg catggggatg gaccggatgg tgcgggcgtt
       61 ggcggtgacg tcctccccgg tctcgccgtc gccgcgcgtg gccgcggtgg tcagaaggcc
      121 gtcctcgtag gtgagcgcca gcccgacgcc gtcgatcttc agttcgacca cgtattccag
      181 cgggtcgccg ggccgcaggc ccttgagcac gcggccgtgc cagtcgttca ggtcgtcggt
      241 ggaataggtg ttgtccaggg agagcatggg caccgcgtgg cggtgcgtgg ggaagccttc
      301 caccggtttg ccgcccacgc gctgggtggg cgagtcgagg gtgcgcaggt ccgggtactg
      361 tttctccagc gcctcgagtt ttttaagcag gaggtcgaaa tcgtaatcgg agatgaccgg
      421 cttggcttcg gcgtaataga ggtgttcgtg gcgggcgatc tcccgccgca gacgttcggc
      481 ttccttgcgg gcggcggcgg ggacgctcac gggactactc tttggccgcc ggcgtcggcg
      541 gcgccgtttt tccccgttcc agtttgatct tgtcgaggat cccgttgacg aatttccccg
      601 aatcctgggt ggaataggtc ttggcgatct ccacggcctc gtcgatggcg acggagacgg
      661 gggtgtccac gtggaacagg agctcgtagg cggtgaggcg gaggatgttg cggtccacgg
      721 cggccatgcg ggagagctcc caattctcgg cgtatttggt gatgagtccg tcgagcaggg
      781 gcatgttctc cagcgcgccg cgggccaaat cgtcggcgaa agtgcgggcg cggggcgcca
      841 gggtgctttc cgtccagcag gccgcggcgg ctttttccag gtcgaacttg ccgacgtcga
      901 cgagatagag gatctgaagg gccgcttccc gggcctgtcg tcgcgcgccc acgttatttc
      961 ccccgccgca ggggccgcag gagccgcgcc gtttccacgg cggcgaccgc gacctcatac
     1021 cctttgttgt cggtgccggt tcccgcgcgc gcccgggcct gggccacgga gagcggcgtc
     1081 agcacgccga acatcaccgg cacgcccgtg gcgagcgcgg cctgggccac gcctccggcg
     1141 gtggcctggg cgatgtaccg gtcgtgcggc gtttcgccgc gcaggatgca gccgagggtg
     1201 atgacggcgt cgtatttttt cgattgggcc aacgtttgcg ccgccagggg cagttcgaag
     1261 gcgccgggca tccaggtcac cgagatgtcc cgcgcgcgga cgccgcattc ggccagtccg
     1321 cgccggcagc cgtccaaaag gcgttccgtg acgggggcgt tgaaccgggc cgccaccacc
     1381 gcgatccgga gcccgcgtcc gtcggccggg gccgcgccgg gacgtttttg atgggacggg
     1441 atcttcatag atgtttgagg agatgaccga gcttgcggcg cttggtctca agataacggc
     1501 ggttcatggg attgggcggg atctccaggg agaggcgttt ggtcaccttc aggccgtagc
     1561 cttcgatgcc gacgatcttg tgcgggttgt tggtgagcag atggacggtg gagaggccga
     1621 ggtccgccag gatctgcgcg ccgatgccgt attcccgcag gtccgccggg aaccccaatt
     1681 tcttgttggc ttggacggta tcgtagccga tctcctgcag gacgtaggcg tggagtttgt
     1741 tcacgagccc gatgcctcgg ccttcctggt gcatgtagag gaggacgccg cggcgggcgc
     1801 gctcgatctg ttgcagcgcg gactggagtt ggtcgccgca gtcgcagcgc agggaatgga
     1861 gcacgtcgcc ggtgaaacag gaggaatgga cccgcaccag cacgttcggc cggcccgcca
     1921 cctcgccctt caccacggcc acgtgatggt cgcccgtcgg gatgtcctcg tagagatgga
     1981 tctggaaatc gccgagccgg gtcgggagtt tggcgctcac cacgcggcgc accagtttct
     2041 cgtgccgccg ccggtgggcg atgaggtcct ggatggtcat cattttcaga cggtatttcc
     2101 gggcgaaccg tttcaggcgc ggcaggcggg ccatggtgcc gtcctcgttc atgatttcgc
     2161 agatcaccgc cgcggggtag agtcccgcca ggcgggccag gtcgatggcc gcctcggtgt
     2221 ggccggaacg gaccagcacg ccgccttcct tgtatcggag agggaagatg tgtcccggtt
     2281 tggagatgtc ctcggggcgg cttttggaat ggatcagcgt cttgatggtg tgggaccggt
     2341 cgtgggcgga gatgccggtg gtgacgccgc ggcgggcgtc gacggaaacg gtgaaggcgg
     2401 cctcgcggga agagccgttt tccaccatgg gggagatctt cagttcgtcg agccgttccc
     2461 ccagcatggg cacgcagatg aggccgcggc cgtgtttggc catgaaattg acggcggcgg
     2521 gggtggcttt ctccgccgcg atgacgaggt ccccctcgtt ttcccggccg gggtcgtcca
     2581 ccaccacgat gggcttgccg cgctcgaagt cgcgcagggc ctccggcacg gagacgaagg
     2641 tttttttggc ggaagggctc attggtattc ttcctcgggg atcagggatt ttttcacgaa
     2701 aaggtcgtcc gtcggggtct cccgccgtgc ggcgaggagc cgatccagat gtttggccag
     2761 catgtcggtc tccaggttca ccgggtcgcc cggttttttg gcgccgaggg aggtgttggc
     2821 ctcggtgtgc gggatgatgg cgacggtgaa agagacctcc ttgatgtcca ccacggtgag
     2881 gctgatgccg tccaccgcca ccgacccctt cggcgccagg tggtccatga ggtgcggcgg
     2941 gacggaaaat tcgtagagga ccgtggcggg gccggccggg gtcttggaca ccagtttccc
     3001 ggtgccgtcc acgtggccca acacgaaatg tccgccgaac tcgtcccccg cgcgcagcgg
     3061 cgtttccagg ttcaccttct ggcccggccg gtagcggccg agggtggtgc gctggtaggt
     3121 ttcggtggag acgtcgaagg tgagcacctg cgggggtttt ttcccggtga aagagaccac
     3181 ggtgaggcag acgccgtcca ccgccaccga cgcgcccttt ttgaggcgcg ggaggcgggc
     3241 ccgaacccgg agtttttccc cgttccggct gaccacgtcg cctaatgtct ggatgatgcc
     3301 ggtgaacata tcaatatttt acaaaaccat gcaccaggat atcgccgccg accttggaaa
     3361 cggtcatgtt gtccagccgg ggggcgctgt tcggatgggg ccatcccccc ccggccacgg
     3421 aagggacggc gtggcgtcct cccaggagtc ggggggccaa aaacaggtag acctcgtcca
     3481 gcagccgggc atccagcagg gccccggaga gacgaccacc gccttctaaa agtatttgat
     3541 taatattcat ttttgccaat aacttaagga ttaacattaa atttatgtcc ccgtttttca
     3601 gcgggcaggt caggagttgg atcccggcct tttccagccg ttttttgcgc ccggccgggg
     3661 gggtggggcc ggtgatgacc caggtcggcg ctcctccctt aaaaatcttt gccgagaccg
     3721 gcaaatccag ggatttcgac accaccaatc ggaccggatc cggccccttc ccgtgactgg
     3781 tcaagcgggg gttgtccttt cgaaccgtgt gggcccccac cagaatggcg tccgattccg
     3841 cccggagccg gtgacccagt tcccgggcca acggcccggt aatccaccgg gaaacgccgc
     3901 tacgggaggc gattttccca tccaaagtct ggcccgcctt gaagagcaca tagggcaagc
     3961 cggtttcgtg gcgtttcaca aaggcccggt tcaaaaagcg agcctcgttc cggagcaccc
     4021 cggtttcgac tcggatcccc gcccggcgga gccgatccag gccgtttccg gagaccgggt
     4081 tcggatcttt catggcggcc accacgcgtt tgatcccggc ccgttgcacc gcgtccgagc
     4141 agggcggcgt ttggccccag tggcggcagg gttccagggt cacgtagagc gtggccccct
     4201 gggcgcgttt tcccgcccgt cggagcgcca ccgcctcggc gtggggtccg ccgaaagatc
     4261 catgccaccc ttcccccacc accgccccgt ttttgacgat gaggcagccc accatcggat
     4321 tggggtgcgt ccggccgcgt ccttcctcgg ccagcgccag ggcgcgccgc atataaaact
     4381 cgtcgtcttt cagggacatg ggggcggggt tcgtttaatt tttttgattt ccggctggac
     4441 agaataagca ttttcaggga catgccccgt ccccgaagca cgagggaaag aatttagctt
     4501 ttttgagtcg aaaaatcaac gcggtccgcg cgggtccgga ggcgggctac ttcaggtcct
     4561 ccgaccggtc ggcggggatt tggaagcgtt ccagtatctc ccgggtggct ttttccgcct
     4621 gggccgcgtc cagaatgatg cgggaggcgt agggcggcga ctccagctcg atgacatggt
     4681 aaggtccctc gggtttttgg agtccttgga gcacgtcccg catttccgcc acaggcacgt
     4741 tgttgacgct tttcacgatg gcgtccctca gccagtagtg gtagcccacg ttgatgttgt
     4801 gcgggagagt tttcgagatg agcaccactt gccggcgttc tttcgtcggc agatcggtga
     4861 aaaggtagtc gagcaccgcc ggcagcacgc tccgcattcc gcgctcctcg atgtagttgt
     4921 gactcagggg catgaacacc agcccgccga aaatgaaata ggtgggaacc gtgtcatact
     4981 gcgggccgga cacgagggtg acgtgtttcc ccagcgccag agtggcgtcg aggcgccggc
     5041 cgtcccggag gattttgagc tgcacgggct cgccgatctg gcgcgtgctg atgaggtggg
     5101 agaactccac gcgttcgtcg ccgcggaggg acaccgtgcc gtcgttggcc accggctgcc
     5161 cgtcgatgga aaggagcacg tcgccgacct ggaaagcgtt cacggcggcg gcgccgtagg
     5221 ggatcttcgt caccaggatg ccgctttcct tcgccgccag accgcaatat tttcggaaag
     5281 tggcgttttc cagcttttgc cagaagatcc ccagggaggg gacgccgtcg tagtcgccgt
     5341 cgtcgacgtc tttcaggaaa cgttggatga tgggcgtggg gatggcgtag ccgatgtttt
     5401 ccgtgcggcc tcccttgagg gattgaaagg agatgcccgc cagtttcccc tctttgaaca
     5461 ccggcccgcc gctgttgccg gggttgatgg ccgcgtcggt ctggatggcc agcagttcgc
     5521 ggcgggagtg ttgatatcgc tgcacctcga tgcgggagac gattccctcg gtgatggaga
     5581 gttcctcccc gccgacgggg aatccgtacg ccgccagttt gtccctctgg cggggcaggt
     5641 cgccgaaact caccggccgg gtgtttttga agaacaccgg gtcgtccacc gagaggagcg
     5701 ccaactcccc gtcgtgggcc acgaactcca ccttcgcctc gtagcgttcc gggtcgccgg
     5761 gtttggagac ctggaggaag acctggttcc ccaccacgtg ggcgttggtg aggatgcggt
     5821 tccccgggag aatgcatccg gagccgctcc cctgttcctg ttctttggtt cgccagggtt
     5881 cggtgtagtc cggttctttg ctgacggtat agatcttcac cacggatttt ttcagggcat
     5941 cctgcgcgtc ggcggcgaag gccgggcgcg gggagccgag cgtttcggcc gcggccagaa
     6001 gggacacgag gatcaagtga aacagggatt tgggtccatg caagagggag cgggtcatgg
     6061 cggtttctcc ggggacggat tttttaaaaa gcggctgtcc gtatcttaac aaaaagaggg
     6121 cggttcggtt caccggccct cggccaggcg gtcggcgagg aacgcgggca gccgggcgtc
     6181 ccgcattttt ttctgcaccg cggcgacgtc gtaggccacg cggaacagcg tcgcctcccc
     6241 ggtttcgtcg tcatacacgc cgcaggcggc gcgcgggtcg ccgtcccgcg gctggcccac
     6301 cgaacccggg ttcagcaccg tcttgtcgcg gggcggtgtt tttatcgttt cgccgttgtt
     6361 ccaggcggaa acttttgcgc cgtcgccgtg gcgtctcagg acgcggggca catgggtgtg
     6421 cccgacaaaa cagaccgggg tctcgaaaag gccttcgttc gctcggaatt ggtccgaggt
     6481 cgtgaaatag tcgtcgatgg ggttttgcgg cgttccatgc accagggtga atcggtccgt
     6541 ggcgacgcgg tccggcaggg aggagaggaa ttttttcgat gccggggtga gttgccgggc
     6601 ggtccagagg gcggcggcgc gggcgtgcgg gttgaaccca tcgaggtcct ttttccccac
     6661 gacggcccgg tcgtggtttc ccgccaccgc ccgcccgtgg ggcagcgctc ggaccgccgc
     6721 caccgtctca tcggggttcg gcccgtagcc gacgatatcg ccgaccacca gaaaaccttt
     6781 gaccccctgt tcacgcaaca gcgtgaggac cgcccgcaag gcctcttcgt tggagtgaat
     6841 gtcggccagg actccgtatt tcacggattt acgggcggtc gaggaggacc cggaaaaaga
     6901 attcgggcag gcggctcatg cggacggcgc gggagggttc ctggagaagc cggaagaacc
     6961 attccagtcc ggcgatcctc atccagcggg gcgcgcgttt caggcggccg gagagcacgt
     7021 cgaagctacc gcccaccccc atggcgattt tgcatttgag ccggccgagg ttttgacgga
     7081 tccagatctc ctgacggggg acgtcgaggg ccacaaagag caggtccggg gcggcccggg
     7141 ccacggccag gattgccaat gattcggttt cgcgtctgaa ataaccattt tcggttccgg
     7201 ccacgcgcag ggcgggatat ttctccttca accgttcggc ggttttttcc gccacgcccg
     7261 gcgcggcgcc gagaaaaaaa acgctccggc ccgtctcggc ggcccggcgg cagagatgct
     7321 cgatgagttc cacgcccggg atcgtggccg gaaacggccg gccgcgccac cggcccgcca
     7381 tcgccacgcc ggcggaatcg ggtacgacca ggtccgccgc gcggaacgcc tcggccagcg
     7441 ccgggttttc ccgggcggcg agaagcatga gggcgttggc cgtgatgagc tgacgcggcg
     7501 cgccggaaga gaggaaggac tcgacgcgcg agagaagggc cggaaggtcg agggaatcaa
     7561 taccgaaccc caacacggag gtccgtgggg agtccggcgg catcggggag aggccggtca
     7621 cgggagacgg accgagcccg agagggaaac gacgttactc cgcgggtttc gtttccggtt
     7681 cctgccggga atcgccggcg atgacggctt ccgggctgag ggtctcggcc agccggccgt
     7741 ccttgcgcag tttggccgag agcagtttgg tgacgcccag ggtctccatg gtgacgccgt
     7801 aaagcacgtc ggcggtttcc atggtgcgct tgttgtgggt gatcatgagg aactgggact
     7861 ggtgcgtgaa cgccttgatc agcgtgatga accgggtgac gttggcctcg tcgagcgggg
     7921 cgtccacctc gtccaggatg cagaagggcg ccggccgcac catgaagaag gcgaacagga
     7981 gcgccacggc cgtgagcgcc ttttctccgc cggagaggag ggcgatgttc tgcagtttct
     8041 tgcccggcgg ctgggcgaac acctcgatgc cggtgttgag gatatcggat tcgtccgtga
     8101 ggcgcaggtc cgcctcgccg ccttggaaga gctggccgta gatggctttg aagttctccc
     8161 gcaccttgtc gaaacagtcg cggaaactgc tccgcgtggt ggcgttgatc ttctggatgg
     8221 tctgcaggag gtcttccttg gctttgagga ggtcctgttg ctgggagaga aggaagttgt
     8281 atcgctgttc cagttccgcg tgttcttcgg gagccgccag gttcacggcg ccgaggctct
     8341 ccacgcgccg gcgcaggcgt tccaggtccg tcagaaccac cgcctgggtc gggagcgggt
     8401 ggcgttcctg ggcctccagc agagtgagac cgtatttgtc tttcagctgg gtctcgaagg
     8461 attcccgttt gaattcggag tggttcgcct ggagtttctt gtcctggagc tgggtgcgga
     8521 tctcctcgga cgagtcccgc agggtcgtga gctcgttctg ggaggccgtc agacggtcgt
     8581 agagcgtctg tttctccgca tggatgcctt ccaacgcctg ttccaattcc ttgcgcttgg
     8641 ccatggaggt ctcgatctgt tggcggcact cctcctggat cttctgttgt tcttcgatgc
     8701 gctgttggga ggtggagcgt tcctgttgtt tggattccag gccggccgcg agggcgtctt
     8761 ggtccgaccg ggccgcgtgg agctgtttct cgcgccagcg gagccgttcc tgttggctct
     8821 gggcgcgctc tcggacggcg ccgaggaggg cgcccgcatc cgacagttcg atctgttctt
     8881 tctgggtccg ttcctggagc agcgtccatt gttcccgcag gcgcgcctct tccagcgtca
     8941 attcgcgcag tttttccgcc agcgcccggg cggtttcttc cgcctggcgg cgttcgtttt
     9001 gaaggcgttc cgattcgccg tcgatcaggc ggatctcgtc ctggaaaagc cccgtttgga
     9061 tgcgggtctt ttcatgttcc tgggcgaaga aatttttctg cacccgggcg gcttccgtgg
     9121 cctggtgcgc cttctcccat tcgtccatgt ggaggcgctt ttggtcctcc agggccgtgc
     9181 gtttggccag ggcgagctcc aactcgttgg cgaggcgcgg ggattcggcc tccagcgagg
     9241 cgcggagcag gggatcttcc ccccgcacgg gcgcgaactc cgccccgccc tcgatcacgc
     9301 cttcgccgta gagcgtggtg ccggcggcga gccagcggct gagcaggaat ttcaagaccg
     9361 gttggaactt ggcgtcggcg cgcaccatct cgaggaggga gcgttgcccc ccgcccgaga
     9421 ggccggcgga gacgtctccc tgcggcagac ggtcgagcac ccacacccgg acccggccct
     9481 ggcggtgatc ggtgacgtgt tgcagggcgg cctgggcctc gtgcagcgag tcggccacga
     9541 ggtcgttcaa atggggcccc atcacttggc ggagcgccgg ctcgtccgcc ggttccacgg
     9601 cgatgagttt gccgagcggg ccgtgcagtc cggacaggcc ggccgacagc accgcgtggg
     9661 cgccctgtga aaaaacatcg tccgcggccc acttctcctg cgcctccaac tgggccttgc
     9721 actggaaaag tttggattgg acctcctccg aggatttggc cagggcggtc aggtccgctt
     9781 ccgtctgggc cagggcggcg cgggcctggg cgacggtctc ttccagccgg cgcagttcgg
     9841 cgtcgcgggc ctggatgtcc tggtcgtatt gcgtcagggc ggtttgcgcg gcctggagtt
     9901 tttcctccga tttggcgcgg tccttctgca acgtcttcaa ttcggtgtcg agccgggtct
     9961 ccagggaccg tttggccccg atctcgttgt ggagttgggt ggcgtcctga ttgtttttcc
    10021 agagggcgtc ctgcagccct ttcatttcgc cggcggtggt tttttgccgt tcggcgagga
    10081 cggcgtagga ttcctcctct ttcttcaggg ccagcccggc ctcctcggcg gtcttggctt
    10141 cggcgtcgag cgcctgttgg aactccgcgc aggccttctc cagttcttcc aggcggacgc
    10201 ggcccttggc gagctcctcg tccagcacgg tgtcgcgctg gcggatggac tcctcccagc
    10261 ggcgggcgtc ggcctgtttc tgttcggcca gcccgatggc gccgtccacg tcggaaatct
    10321 cccggttctg cgccaccatg cgttcgccca gctgggtctc cgccagacgc agttccgcca
    10381 gttccgcctc gcgctggttg atcagcgtgg tcttggactg gaggtcttct tccatgcgct
    10441 ggatctcctg tttcagcgcg gtgatctgct ggtcgagttg agacgtctcc cacagcgcgt
    10501 gggagatctc catgaccttc aaatcttcct gggctttctg atacagacgg gccttgcgca
    10561 ccgccgcctc gatcttgtcc atctgttcct tggtcatggc gatgacgtcg gccagccggt
    10621 cgaggtcgag ctgggtgcgt tccatcttcc ggagggcttc ttcgcggcgg gccttgtatt
    10681 tggacacccc cgcggcctcc tcgaaaagtt cgcggcgttc ctcgggccgg gcgctgagca
    10741 cgtactccac ccgtccctgt tccatgatgg aatacccctc ttcgccgatg ccggtgtcga
    10801 ggaagagttc gcggatgtcc ttgaggcggc actgcgtctt gttcatgaaa tattccgact
    10861 cgccggaacg gaacagccgg cgggtcacgg tgacctcgga gtagtcgatg gggaggcggt
    10921 tctgggagtt gtcgaacgtg agggagactt cggccatgtt ggagggcgcc cggttggccg
    10981 aaccgttgaa caccacgtcg agcaaaattt tggaacgcag ggatttggcg gacatctcgc
    11041 ccagacacca gcggatggcg tccaccacgt tggatttgcc gcagccgttc ggaccgatga
    11101 cggcggtgat gcccggttcc agatcgagcc gggtgcgatc cgcaaaagac ttaaacccca
    11161 ccatttccat gcgttttaag tacatctttt gccatccttt cccattacgg gtatgtttat
    11221 tgcttagcag tatattttac acataccctc ctttttgtca agaaaataat cgtctcaagg
    11281 gcggaacata taaagatgtt tccctgccat cgtccggtcc gggaaatctc gcgggattac
    11341 aaagctttca accgttgagg acgggacggc gttccgggcg cgtttccccc cggttcgctg
    11401 tttccaattc cgtccggttt cgatatgatt atgcctatga aacggaaaat cgcggtgcgc
    11461 gcgccgaact ggctgggcga tgtggtgatg tgcaccccgt tccttaaacg gttgatcgag
    11521 aagaacccgg aggccgaggt tcacgtcctc tgccgcccgg cccacgggga gatcttttcc
    11581 cgtttccccg gcgtcaaagg cgtccggctc ttggggaaag acgaaccgtg ggcggccctg
    11641 gtgcggcgcg tggcggcgga gaagttccag accggctacg tcctcccccc gtccttctcc
    11701 tcggcgctcc tctttttcat ggcgggggtg ccggaacgca tcggttacgc cacggatttc
    11761 cggaggttct ttttgacgcg ggcgttggcg ctggacgagc ggttccatta cgtgcggcgg
    11821 tatctggggc tgttgggcga ggagtcccgc gaggcggcga cgtcggatct tttcttcccc
    11881 gccccgccgg acgcggcgca cggcgcgcgg gcgtttctct tggcgcgcgg gttttccttc
    11941 aaaacgccgt gtctggccgt ggcgccgggg tcccaggctc ctggccggcg gtgggagccg
    12001 gaacggtttg ccgcggtcat cggcgcgttg gccgagaagg actggccggc ggtggttctg
    12061 gtgggcgcgc cgggcgacct cccctgggcg gaaaaagtgc ggcacgcctg ctcgcggccg
    12121 gtgatcaacc tgtgcggcgc gacggacctg gtgacgttgg gggacctgct ccgccagtgt
    12181 tcggcgctgc tcaccaacga gtccggcatg atgcacgtgg cctgggcggt gggcaccccc
    12241 acggtggcgc tggccgggcc gtcggaaccg cgggtcacct cccccttcgg ggcgccggtg
    12301 cgcgtcctgc agcaccggga aatcccctgc gtgccgtgca tcaggaacga ctgttacctc
    12361 gccggggacg attataaact ttgcctgaaa tcgatcgccg tttcggaggt ggtgtcggcg
    12421 ttgaacgagg tcgtcaaaac gtcctgagtc ccgaccgctt tccttgagaa taaaaaaacc
    12481 cggaagagga tttccgggtt ttcacttttt caggcggcct gaaacgggtc agtgcccctt
    12541 gcgtttctcc agttccttga ccaaggccgg gatgaattcg aagaggtccg cctcgatgga
    12601 gagcgtggcc agtttcatca gcggggcctc cgggtccttg ttgacgcaga cgatgacgtc
    12661 gctggagttc atgcccgcga ggtgctgtat ctggccggag atgccggcgg cgatgtagag
    12721 tttgggacgc acggtgcgtc cggtgagacc cacctggtgt cggtaggtga tccagccggc
    12781 gtccacggcg gcgcggctgg cgcccacggc gcccccgagg atcttcgcca gcgcctcgat
    12841 cggtttgaag ccggccgccc cgcccactcc gcgtccgccc gccacgatga cgtccgccgc
    12901 ggagatgtcc tgttcctcgc cctgttcggg ttcatacttc acgaaacggg tgcgctgctt
    12961 gaatttggac gggtccaccg gcaccgggat ggttttcccc tggcggccgg gctttttttc
    13021 cgaccggggg aacgacatgg gccgcaccgt ggccatctcc gggcgctgtt tttcgcacag
    13081 gatggtggcc atgaggttgc cgccgaagga cggccgggtg gcgtgcaaaa gacgggtctt
    13141 ggggtcgatg gtcagctccg tggcgtcggc ggtgatgccg gtcttggcgg agatggccag
    13201 ccgcgaggcg aacgaacggc ccagggtgga ggcgggcagc aggagcttgt tgggtttttc
    13261 cttcaggatc aggtccgcga aggcctgggt gtagagttcg tccacgaaat tttcgaagca
    13321 ggggtggtcg agcacgtaca ccttgtcggc gccgtggtcg atgatgtcct gggcgtgccg
    13381 ggccacgtcc ttgccgatga gaacggcgga gatgtcttcg cccagcgtgc ccgcgagttt
    13441 ctgggccaaa gagagcaatt cgtagatggt ggggaccagt tttccgtgcc gcacctcggc
    13501 atagacccac acgcccttgt agagggaggt gtcgatggcc ggcccggtgg aggtcgcggt
    13561 ctccttggtc atggtgatgg cgttgaaaag gtccttgtgg gcggatttgg cgccgaggtt
    13621 gtcgcagact tccacgcagg cgttgcaaaa gacgcatttg acttcgtcga tgacggcgag
    13681 ctttcgccac ttcacgcctt cgttcgggtc gcggtccacc atggtgatgc aggagacggg
    13741 acaggccttg acgcaggcgg tgcagccggt gcagcgttct ttggtgacga aaatggagtt
    13801 cggcatggtc agatgagctt cagttcctga agtttgtcca cgagcttctt tgctttttcg
    13861 tcgacggtgg cgccgtccag ccgcaccccg ccctggcggg gcggcgggtt aaaggatttg
    13921 gacaccacgg tgggcgagcc ggccagcccg atgttggact tgtcgcactc cagcgccacc
    13981 tcgtcccatt tggggatggc ggctttcttg gcggccattt tccccttcag cgacgccacg
    14041 cgcggggtgt tgatctcctt caccaccgcg atcaccgcgg ggaggtccat ttccaaaatg
    14101 tcggtgccgt cctccatcat gcggtgcacg acgattttct tgtcgtcgac ctggtccact
    14161 ttcttgatgt aagccacgtt gggccagtcg agccaggcgc cgatgccggg ccccacgtgg
    14221 ccggtgtccc cgtcgttggt ctgcttgccg cagatcacca ggtgcacggg gcctttttcc
    14281 ttggcgatct tcttgatgcc catgtgcagg gtgtaggagg tggcccaggt gtcggcgccg
    14341 gcgaaggcgc gggaggtgat gtggaaggcc tggtcgcagc cgcgggcgat ggcgtcccgg
    14401 agggaatcct ccgcctgcgg aggacccatg gtgatgaccg acaccgtggt gccggggacc
    14461 cgttccttga tgcggacggc ttcctcgatg gcgtactcgt cgaacgggtt gatggcggcc
    14521 gccatcccct cccgtttcaa cgtgccggtg gccgggtcga tctggatgtt gctggtggcc
    14581 ggagtctgct tgatgcagac gacgatgtga aggcccatgg atgcgcgatc tctttcgtct
    14641 aaggtgattc cgtttttaca cggagcccag atagtaccac aaataccggg aagacttcaa
    14701 gcaacaggtc ccccgccgcc cgggggcgac gcggattatt tccccgaggc cagctccttg
    14761 gtgatcatgg cgccgatctc gttgcgctgg atctggttgg tcccctcgta gatctgggtg
    14821 atcttggcgt cgcggtggaa tttctcgatg gggaaatcct gcatgtagcc gatccccccg
    14881 cagaggtcga tgcacttgcc ggtgacccag tcggcggtgt cggaacagta gagcttgcac
    14941 atggccgagt atttggtcag ccgctgtttg gcgacttttt tgagttcgtc gtagaccagg
    15001 gtgccgttgg cttcggacgc ctccacggcg gctttgaagt cgatgtccat ggcccgcgtg
    15061 acggaataga ggagggcgcg ccccgtttcc aggcgggtcg ccatgtcggc caccaggtgc
    15121 tgggaggact ggaaactcaa caccgactgg ccgaactgct ggcgcatctt ggcgtaggcc
    15181 agggcttcgt tgaaagcgcc cgtgccgatg cccaacgcct gggacgccac gccgggccgc
    15241 gacatgtcga aggtggcttg ggcgacgaag agcccgtgcc cttctttggc cagcaggttc
    15301 tgggccggca cgcggcagtt gttgagcacc acctcgtagg tggcgctgga gcgaatgccc
    15361 atcttcgttt ccttcttgcc gaaggagagg ccgggcgtcc ctttctcgat gatgaagcag
    15421 gagatgccgc ggggcccgcg cgaggggttg gtggaggcga acacggtgta ggtctccgcc
    15481 acctcgccgt tggagatgaa acacttggtg ccgttcagca cataggaatc gccgtccttc
    15541 ttggcgatgg tcttctgggc ggtggcgtcc gaacccgaag cgggctccga cgccaccgcc
    15601 cagaagacca ccgcc
//
LOCUS       DAOTFD010000037        15469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2608_length_15469_cov_5.012975, whole genome shotgun sequence.
ACCESSION   DAOTFD010000037 DAOTFD010000000
VERSION     DAOTFD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15469
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_15469_cov_5.012975"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="ssb"
                     /locus_tag="P5079_03870"
     CDS             complement(<1..173)
                     /gene="ssb"
                     /locus_tag="P5079_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY29156.1"
                     /translation="MTSNLRLPALNDVRLVGRLTRDPELRFTTKGTGVCRFDIAVNRR
                     FKAGNGEWQDETS"
     gene            complement(163..552)
                     /gene="rpsF"
                     /locus_tag="P5079_03875"
     CDS             complement(163..552)
                     /gene="rpsF"
                     /locus_tag="P5079_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRY29157.1"
                     /translation="MQVYETIFISPVDLAVQKQDDLVEKVKSMITKAGGEIVLVEKWG
                     RRRLAYAIKRHREGFYVYLKFKAPSAILAELNQFYRVSDDVVRQLTCKAVRTRPGVVA
                     PAAPAPAAEAAAPVAAEPVAKEVSHDK"
     gene            complement(735..1568)
                     /locus_tag="P5079_03880"
     CDS             complement(735..1568)
                     /locus_tag="P5079_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29158.1"
                     /translation="MKFLRMMTALALTVGVLGCGNNKSADKVLSGVHMTGDGVSGGGT
                     GSEGGGGLSGGNSAAVNSAGAQGALVGVNTVGQASQGLSGVIPGAPGVRAPAPFGATY
                     TSAGDGYYWIEAVQPDGDYSKIKCLCPAGTVFDPNDVVNFTSPGLGTALVEYFERSEE
                     DGSETLGRFTFSTVGGNLNSLTMTGNIKLTFGDGAVNYTVTDFALDGRGNATAGKLVG
                     SGSFPSMGISSLAMELNVTSANTITGTATINGQVLQIYVGPDGVGYYTDQTGTHVIPE
                     V"
     gene            complement(1597..2481)
                     /locus_tag="P5079_03885"
     CDS             complement(1597..2481)
                     /locus_tag="P5079_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRY29159.1"
                     /translation="MKKSWLSMALLALVATTVRAGELLVTEPVSMGEAKSVSVELRPT
                     YRMDLWKIKNAVPACEVTNSFLRTDINARWVPVEKWEVLLEVPYVSDKVETDPSDPTD
                     DTDSGLGQVILGGKYAFCSMLGAALRVELPTADSDKYLGEGLNTGLSLLLEKPMGAVT
                     LYANAGYLFKWNYKTTLDVKNGVEYQVDPGDIVQGNLGITHERWGMNWIGEVNVNVVG
                     REQNDGNTARLMKDVEASAGTAVDLVLGVSKKLNDKLGVKGGVAWGVGEEKFRSLDAV
                     RGAGDYKIILAGDYRFGY"
     gene            2753..3409
                     /locus_tag="P5079_03890"
     CDS             2753..3409
                     /locus_tag="P5079_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY29160.1"
                     /translation="MDEPRRQEFHQKALPLLNELYGVALRMTRNPQAAEDLVSEAYAR
                     AWKSLDQFQPGTNIRAWLYKILTNTYINAFRKKHREPEKVSMDAYERIEDFHFYNRIA
                     SQAGKSPDPFDSVVGKLTNEQFEKALESIPDEYRAAVVLYDLQGLSYQEVSESLEVPL
                     GTVRSRLSRGRSLLQSALWKHAEDAGIVKHNGGDSPRWVKPLNYFFKSKPGTENVKSA
                     "
     gene            3426..3653
                     /locus_tag="P5079_03895"
     CDS             3426..3653
                     /locus_tag="P5079_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29161.1"
                     /translation="MSNCDKVLHHIYEYLNTHDVTDALSDEIREHLEKCRHCYDRVTF
                     EKRLLERLRRAGGAPCPETLKKRIETLVKNF"
     gene            3696..5162
                     /locus_tag="P5079_03900"
     CDS             3696..5162
                     /locus_tag="P5079_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29162.1"
                     /translation="MTTSYIVIAAYILILFLLSLYGVHRYWILYLYWRHYKKAPPLPT
                     PFSPVSWPMVTVQLPVYNEYYVVRRLIDAVCRLDYPKDRLEIQLLDDSTDESRELARG
                     LVEERRREGFPVQYLHREHRNGFKAGALAEGLRYAHGEFIAIFDADFLPSPDFLRRTI
                     PHFAEERIGMVQTRWGHLNSDYSFLTRAQALFLDGHFMLEHTARNRSGAFFNFNGTAG
                     VWRRRTIEDAGGWADDTLTEDLDISYRAQLRGWKFLFLPEVVCPAELPVDIVAFRSQQ
                     HRWTKGALQVAKKVLPAIWKSPLPFFVKLESTVHLTSNLGYCLMFALSLLLLPSLMAR
                     NTLAWPQAVNLLEFFVFLLTALSITTFYAVVQRETKAARFSLADLPPLLAFGIGMCFN
                     NTRAVWEALCSIPTEFRRTAKFNIQTRQDTWRNKRYRAARKRTGLVEALLAVYLLITL
                     YQSLSGTAAWMTLPLIGLFLGGFAYVGWLTFYHALQKN"
     gene            complement(5554..7710)
                     /locus_tag="P5079_03905"
     CDS             complement(5554..7710)
                     /locus_tag="P5079_03905"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29163.1"
                     /translation="MALSTEEAKTGGSFAAAGLFLAAFAAYGTGAMPSLYGGDSGELI
                     TSALALGVSHAPGYPLHALMGKLFSNVLPWGGPAYRVNLFSAFLSAATGVVLFSALRD
                     LLKNAVAGAAGALVYAALPIVWDQAQGTEVFALNNLLAALLILFFVRAFPSDGRLRPR
                     WLAGGGFLYGLGLANHQTLVFLAPGFVWAAGRFFWERGAGAAENLKRAARSATDWLPA
                     ATAFFLLGLSVYLYLPLRAARHPPVNFGDPHTLRRFVDVLTRKEFGRLELHPAALPFH
                     TAGTVAAQLKSFFRQNVAAGGGFVLALGLLGFLLSSRRPDPAADVRRATSSVSRGAWL
                     LFWILPGPFFYVFSNLSPFNTLAQWRMERFLLLPMLFLAAGAALLTERLLSKRRTGLA
                     IVLLGAVCVEQVFFRPHPWFRWNLGFRDFGRNLCSSLEPRSLLLIDRVMFDEPTSCLM
                     NRLLVERQRPDVRPIYRPGTLFELFYGEDILEIHRDRRYHRQQEREDALWRTLDRPLS
                     VMAFVRDNLPPGDFVLNGLLFQEKKTGRPSDFYIWRGRGPHSPDDYPSRLIRVHYPYL
                     AAKAGFEENDVAGAERYSAESLYVGRDMEWLFSNIGSLWSRWEPVAPELKVRAMRESG
                     RAFSRAVGTDPFFPQGQFGAGYVELQRKNYAGAVDAFAAAARLRPDWPEAHYMLGLAY
                     NFKGLRAEARRAWERFLALDPRSALASDVRRELSGS"
     gene            complement(7710..9446)
                     /locus_tag="P5079_03910"
     CDS             complement(7710..9446)
                     /locus_tag="P5079_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29164.1"
                     /translation="MKKIFPASLFLIFFGFYAYLSFPALSSFRDAGDFSAAALSFGVP
                     HPPGYPSYVLLAKFFQPFLSGNPAYRANIVSAFCGAGVVLLVFLSLSRFCGLAASLLG
                     ALFIGFFSPHMAQSGLSEIYGLNALFAALLLWGAFFIPALSPSARTRGWVGLGLLGGM
                     GMGNHQTLLAVGPSVLLLLWRERGSLVLHPRFVRVVLAAAGLGLAVYLLLPLRAAAEA
                     VQVWGEPDTWNGFWAMFTRADYGTGTLSSLYSTASPAMGLAFWFRVWGRRWGAWAVVL
                     QLAAAVWLFRKENRKVWGAPVLLLWIFSGPVFALLARLQPAEHSEAVLSPALVLPGLA
                     AALGMGFFLEALLKRRRFLAVLGLLAVTLFVVGRGVPAVLSQRQRWNLVSMDYGRNLF
                     RTVPPASVLLMISDTSVFSAAYSQAAGARSDVRLLVDASPSWRWRQYRRRFPDLFSAG
                     QEDGGMELLRRQSGRVGLFTEGIQPKFVESLHPYGLAAAVEWPKRTPACLDRMEESLP
                     LWGFYVRRLPSAAVLRADYYTRTLLKTVSSGGFNAGLLLHEGGRRESSLAFYARSLFW
                     NPERILRWAEIF"
     gene            complement(9591..10013)
                     /locus_tag="P5079_03915"
     CDS             complement(9591..10013)
                     /locus_tag="P5079_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29165.1"
                     /translation="MKKILVVDDDKNLAELIAEFCRDAGLDTRFLTDSSKAVELAAEW
                     KPDLITLDIEMPGVDGIEVLRRLQGDERTNRIPVVIISVVAKGALTEGILKGARMVFE
                     KPLRFQKLIKQLQDLLSRPAQADAESVHFEPHLRTSAP"
     gene            complement(10220..11278)
                     /locus_tag="P5079_03920"
     CDS             complement(10220..11278)
                     /locus_tag="P5079_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY29166.1"
                     /translation="MSSQENKQSKSGLRRVTMKDVAEHCGVSLSTVSLVLSGDQRIPP
                     DTTQRVLEVVKALGYRPNLIARNLAKQRSRTLGVVLPDLGAVVDHSFFTEALKGVYSE
                     TSRQGYRLLLEIATPQFLSRRFYLRLLKENSVDGIVYLGTTLKDDFLREIEPYQYPFL
                     MVGGYLDDAQLSYVAVDNEAGAHMATKHLIDLGHRRIGHIAGSAAVSSARDRLRGYRR
                     ALEEAGIPFEPTLVVDGHYKADVAQEVCRGLLANKVTAVYAGSDWMASGAFRAIKEAG
                     LRIPQDVSVIGSGDLDLARLVEPPLTTVKYDISMVAADGTRRIIQQIESGEPPAVFQE
                     KYPVQLMVRQSSSPPKNV"
     gene            complement(11289..11720)
                     /gene="atpC"
                     /locus_tag="P5079_03925"
     CDS             complement(11289..11720)
                     /gene="atpC"
                     /locus_tag="P5079_03925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRY29167.1"
                     /translation="MARPFPLSVVTPEKVSWEGNVDSLVIPAHEGQLGILPGHAPLLA
                     QLTPGVVRIRQGEETRLLAVSGGYVEVFGGKVSLFAETAELESEIDAERARQAVSKAR
                     SDLRGGAGEPGGSEQTMAALRLALARLRVADRLRRPPPHSH"
     gene            complement(11727..12479)
                     /locus_tag="P5079_03930"
     CDS             complement(11727..12479)
                     /locus_tag="P5079_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRY29168.1"
                     /translation="MNDQGISGEVAGRGLSLNSLWAWAVRLVTAPKQFFSALAKEGGY
                     ATPILYALFWLYVSTVLELIIGRLRTSPVRLGWTAEIVWLVFGPFILLGMGFLVSAVL
                     FVLWHLMGSKENYATAFRCWAVISPLAVLGAVLGLVPFLNALAFLYGVFLLIIASMET
                     HKISPKKAWAVFGAIGVAFVILILATANIRSELERQGYLNQPGMPGGVPPFAGQEAGE
                     PPSVENVANEIQRQIQEQMAKQGAPAPAAKKK"
     gene            complement(12501..13922)
                     /gene="atpD"
                     /locus_tag="P5079_03935"
     CDS             complement(12501..13922)
                     /gene="atpD"
                     /locus_tag="P5079_03935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRY29169.1"
                     /translation="MNQGRVVQVIGPVLDLEFSPGSLPAIHNAVRVPHESGGERRLLT
                     VEVAQHLGDNIVRAVALGPTDGLRRGLAAEDTGAPISVPVGKECLGRLMDLLGEPKDH
                     RGPLKGSTRLPIHRAPPAFVDQTTTPQVFETGIKVIDLLEPYMKGGKVGLFGGAGVGK
                     TVVIMELINNVAKQHGGVSVFGGVGERSREGNDLWLEMQKSKLSDGQTVLSKTVLVYG
                     QMNEPPGSRLRVGLSALTQAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSA
                     VGYQPTLGTEMGELQERITSTTKGSVTSVQAIYVPADDLTDPAPATTFAHLDATTVLS
                     RPISELGIYPAVDPLDSTSRILDPKIVGDEHYAVARSVQRILQRYKDLQDIIAILGID
                     ELSDEDKTVVLRARKIQKFLSQPFHVAEEFTGRAGRYVPLKETVRSFREVVEGRWDHL
                     PEQAFFMAGGIDEVVEKAKSLES"
     gene            complement(13939..14268)
                     /locus_tag="P5079_03940"
     CDS             complement(13939..14268)
                     /locus_tag="P5079_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY29170.1"
                     /translation="MERRRFRRIPADMLVELHDVGGQSARGRAQVRNFSMGGMAVEGH
                     VPLRSGETLFLKVNVPIEFLGKVVYMRSRGGRMNYGIRFQNIGFFDKLRLRRYVTAKF
                     GREIERE"
     gene            complement(14295..15188)
                     /gene="atpG"
                     /locus_tag="P5079_03945"
     CDS             complement(14295..15188)
                     /gene="atpG"
                     /locus_tag="P5079_03945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRY29171.1"
                     /translation="MASLRELRRKVKSVKSTQQITKAMKMVSAARLRRAQGRILSARP
                     FAQKMETLLTDIFSAVAEPGQSGAAVRTVLGHPLLAERKTGPRGLVLITADKGLCGSF
                     NTNLIKRGLEFLRENEGETVELFCVGRKGRDFFRRMKVAVRGEYVNIFNQLSYAHAEL
                     IGRDVTDFFVNRPARDVTVIYNEFKSVMQQRPVRSVWLPLTPPVPASVRPAAPDFLYE
                     PARERLLESLFPRALKAQIFRALMESAAAELAARMTAMENATRNAKELIESLTLYANK
                     LRQAAITKEISELVGGAEALQ"
     gene            complement(15202..>15469)
                     /locus_tag="P5079_03950"
     CDS             complement(15202..>15469)
                     /locus_tag="P5079_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29172.1"
                     /translation="VQVEAVLAAPVDKERDFFVVTGKPKDIMNNVGVYFDPEDMSTKL
                     EVYLADPGQWLLIEGQLSVDKAPNAYKTYSGYALKAKSVKKISK"
BASE COUNT         3063 a         5226 c         4507 g         2673 t
ORIGIN      
        1 aaggacgtct cgtcctgcca ctcgccgttg ccggccttga aacgccggtt gacggcaatg
       61 tcgaaccggc agacgccggt gcccttggtg gtgaagcgga gttccgggtc ccgcgtgaga
      121 cggcccacca aacgaacgtc gttgagggcg ggcagacgaa gattacttgt catgggagac
      181 ctccttggcc accggttccg ccgccaccgg cgcggccgcc tcagccgccg gggccggggc
      241 cgccggcgcg acgacgccgg gccgcgtgcg gaccgctttg cacgtcaact ggcggaccac
      301 gtcgtccgac acgcgataga actggttcag ttccgccaga attgccgacg gggccttgaa
      361 cttcaggtac acgtaaaaac cttcgcgatg gcgcttgatg gcataggcca ggcgacgccg
      421 gccccatttc tccaccagca cgatttcgcc gccggctttg gtgatcatgg attttacttt
      481 ttcgaccagg tcgtcctgtt tctggacggc cagatcgacg ggggaaataa agatagtttc
      541 gtagacttgc acttgggttt gcctccttta tggacttccc cgcctcgcgg caggggaggg
      601 ttccggggca cggtggcggg ccggaaccaa agaggcgtct attctaccag atgccccgac
      661 caaaagacaa tgaaaaagag gagggggcac gatcgccccc tcctcttttt tgtgaagcgt
      721 tttttgatcg gcgatcaaac ttccggaata acgtgcgtcc cggtctgatc cgtgtaatag
      781 ccgaccccgt ccggcccgac gtagatctgc aggacctggc cgttgatggt ggccgttccc
      841 gtgatcgtgt tggcggaggt cacgttcaac tccatcgcga gggaactgat gcccatgctg
      901 gggaacgaac cgctcccaac gagctttccc gcggtggcgt tgccgcgacc gtccagggcg
      961 aaatccgtga cggtgtagtt cacggcgccg tctccgaacg tcagcttgat gttgccggtc
     1021 atggtcaagg agttgaggtt tccgccgacg gtggaaaagg tgaaccgtcc caacgtctcc
     1081 gacccgtcct cctcgctcct ctcaaagtat tccaccaggg ccgtgcccag acccggagac
     1141 gtgaagttca ccacatcgtt cgggtcgaag acggttccgg ccggacacag acacttgatc
     1201 tttgaatagt ccccatccgg ctggacggct tctatccaat aatacccgtc accggccgac
     1261 gtataggtgg ccccaaaagg agccggcgca cgaacccccg gagcccccgg gatgactccc
     1321 gacagtccct gcgaggcctg cccgaccgtg ttcactccca ccagagcccc ctgcgccccg
     1381 gcggaattaa ccgccgcgga gttccctccg ctcaggccac cgcctccttc gctgccggtg
     1441 ccgccgcccg agacgccgtc gcccgtcatg tgcacgccgc tcaggacctt gtccgcggac
     1501 ttgttgttgc cgcaacccaa cacgccaacc gtcagcgcca gggccgtcat cattctcaag
     1561 aatttcatgg tttatcctcc aaagataatc gccggatcag tagccgaacc ggtagtcgcc
     1621 cgccaggatg atcttgtagt ccccggcgcc gcggaccgcg tccaaactgc ggaacttctc
     1681 ctcgcccacg ccccacgcca cgccgccctt cacgcccagt ttgtcgttaa gtttcttgga
     1741 aacccccagg accagatcca cggccgtgcc tgcgctggcc tccacgtctt tcatgagccg
     1801 agcggtattg ccgtcgttct gttcgcggcc gacgacgttc acgttcactt cgccgatcca
     1861 gttcatcccc caacgctcat gggtgatgcc caggttgccc tgaacgatgt cgcccggatc
     1921 gacctgatat tcgacgccgt ttttgacatc gagggtcgtt ttgtaattcc acttgaacag
     1981 gtatccggcg ttggcataca acgtcaccgc gcccatgggc ttctccagca aaagggacag
     2041 cccggtgttc agcccttctc ccagatactt gtccgagtcc gcggtgggca gttccacgcg
     2101 cagcgccgcg ccgagcatgg agcaaaacgc gtatttgccg cccaggatca cctggcccag
     2161 gccggagtcc gtgtcgtccg tgggatccga cgggtcggtc tcgactttgt cggaaacgta
     2221 aggaacctcc agaaggactt cccatttctc caccggcacc cagcgggcgt tgatgtccgt
     2281 ccgcaggaag ctgttcgtca cctcgcaggc cggcacggca ttcttgattt tccacaggtc
     2341 catgcggtaa gtgggccgca actccaccga cacgctcttc gcttccccca tggacaccgg
     2401 ctcggtgacc aagagttcgc ccgcgcgcac ggtcgttgcg accagggcca gcagggccat
     2461 gctcaaccag ctttttttca tgacgaagac tcctttgttt gacttgatat aaaaacgagg
     2521 gtatttggtt cgccgcggcg gaccaagcac cctcgttctt ccaatgaggc tccgatttta
     2581 acagaaaaga gatcgggtgt tcaacggttt tcttttttgt cgtttggaat gtttttgggg
     2641 gaaacggcgg tcgttgtttt cgtttgtctc gatccttgag aagtgataaa atttccccga
     2701 agatgcgccg gggcttttct catgttcccg tcgcagagga acagaccgcc ccatggacga
     2761 accccggcgc caagaattcc accagaaggc gttgcccctt ctgaacgagc tttacggcgt
     2821 ggccctgcgc atgacgcgca acccgcaggc cgccgaagac ctcgtctccg aagcctacgc
     2881 ccgcgcctgg aaaagcctcg accagttcca gcccggcacc aacatccgcg cctggctcta
     2941 caagatcctc accaacacct acatcaacgc cttccggaaa aaacaccgcg aaccggaaaa
     3001 ggtttccatg gatgcctacg aacgcatcga ggatttccat ttctacaacc gcatcgcctc
     3061 ccaggccggc aaatcgcccg accccttcga ctcggtggtg ggaaaactca ccaacgaaca
     3121 attcgaaaaa gcgctggaaa gcattccgga cgaataccgc gccgccgtgg tgctctacga
     3181 ccttcagggg ctttcgtatc aggaggtgtc cgaatccctg gaggtccccc tgggcacggt
     3241 gcgatcccgc ctctcgcgcg ggaggagcct gctccagtcc gccctctgga aacacgccga
     3301 agacgccggg atcgtcaaac acaacggagg cgactcgccc cggtgggtga aacccctcaa
     3361 ctattttttc aagtcgaagc ccgggaccga aaacgtgaaa tccgcatgaa aggacggtga
     3421 ggaaggtgtc gaactgcgac aaagtgctgc accacatcta cgaatatctc aacacccacg
     3481 acgtcacgga cgcgctgagc gacgaaatac gggagcacct ggagaaatgc cgccactgct
     3541 acgaccgcgt gacgttcgag aagcgcctcc tcgagaggct ccgacgcgcc ggcggcgctc
     3601 cgtgcccgga aactcttaaa aaacgtattg aaactctcgt caagaacttc taaactgccg
     3661 ccaatgacgg gacacctccc gcgcgtgaac gaagaatgac gacaagctat attgttatcg
     3721 ccgcctacat cctcatcctt ttccttctgt ccctctatgg ggtgcaccgc tattggatcc
     3781 tttacctcta ctggcgccac tataaaaaag cgccccccct gccgacaccg ttctctcccg
     3841 tttcctggcc gatggtcacc gtgcagctgc cggtgtacaa cgaatattac gtggtgcggc
     3901 gcctcatcga cgccgtctgc cggctcgatt acccgaagga ccgcctggag atacagctcc
     3961 tggacgactc caccgacgaa agccgcgagc tggcccgcgg tcttgtggag gaacgccggc
     4021 gggaggggtt ccccgtgcaa tacctccacc gggaacaccg caacgggttc aaggccgggg
     4081 cgctggccga aggattgcgc tacgcccacg gcgagttcat cgccatcttc gacgcggact
     4141 tcctcccgtc gccggatttc ctccgccgca ccatccccca ttttgcggag gagcgcatcg
     4201 gcatggtgca gacccgctgg ggccacctca actccgacta ctccttcctc acccgggccc
     4261 aagcgctctt cctcgacggc catttcatgc tggagcacac ggcgcggaac cgttccggcg
     4321 cgttcttcaa tttcaacggc acggcgggcg tctggcggcg ccgcaccatc gaggacgccg
     4381 gcggctgggc cgacgacacc ctcaccgagg acctggacat ctcctaccgg gcccagctcc
     4441 ggggctggaa attcctgttc ctcccggaag tggtctgccc cgccgaactg ccggtggaca
     4501 tcgtcgcctt ccgctcgcaa cagcaccggt ggaccaaggg ggcgctgcag gtggccaaaa
     4561 aagtgctccc cgccatctgg aaaagtccgc tccccttctt cgtcaaactc gaaagcaccg
     4621 tccacctgac ctccaacctg ggctattgcc tcatgttcgc gctctcgttg ctcctgttgc
     4681 ccagcctgat ggcgcgcaac accctggcct ggccccaggc cgtcaacctg ctcgaattct
     4741 tcgtcttcct tctcaccgcg ctgtccatca ccacgttcta cgcggtggtg cagcgggaga
     4801 cgaaagcggc gcgtttttcc ctcgccgacc tgccgccgct gctggcgttc ggcatcggca
     4861 tgtgtttcaa caacacccgc gccgtgtggg aagccctctg ctccatcccc accgaattcc
     4921 gccgcaccgc caaattcaac atccagaccc ggcaggacac ctggcgcaac aaacgctacc
     4981 gcgccgcccg aaaacgcacc ggcctggtgg aggccctcct cgccgtttat ctcctcatca
     5041 cgctctacca gagcctttcg gggacggccg cctggatgac cctgcctctc atcggcctgt
     5101 ttctgggcgg attcgcctac gtcggttggt tgacttttta ccacgccctg caaaaaaact
     5161 gattttttcg ggaacttttc ggctccctcg gcgttgtcat gggtgaaaac gatctttaac
     5221 ctcaaaaaat gggaggcctc catgttcggc cctggctgcg gatgctaaaa aacaggggaa
     5281 cggatcatcc gttcccctgt tttcattttc agtgagtatc ccttgagggc aagcagcttc
     5341 tcggcctcct tcggccccca ccaccgaaca cgtgccccgt ccgacaaacc gggacgttgc
     5401 cgagcggcaa cacgggacct gtgttacagg tcccgccgcg acgtcgagaa ccggtgggaa
     5461 gctgaaatcg atttacttgt ttttaagcga caatcccctc cttttccata aaatccggtc
     5521 ggccgatcct cttcctatat agaccgccgt ccctcaggaa ccggacagtt cgcgccggac
     5581 atcggaggcc aacgccgaac gggggtccag cgccaggaac cgctcccacg cccgccgcgc
     5641 ctccgcccga agtcccttga aattgtacgc caaccccagc atgtagtggg cctccggcca
     5701 atccggccgc aggcgcgcgg cggccgcgaa cgcgtcgacc gcccccgcat agtttttccg
     5761 ctggagttcc acatacccgg cgccgaactg cccctgcggg aaaaacgggt cggtgcccac
     5821 ggcccgggaa aaagcccgcc cggactccct catcgcccgc accttcagtt ccggcgccac
     5881 aggctcccag cggctccaca aagacccgat gttggagaac agccactcca tgtcccgtcc
     5941 cacgtagaga gactccgcgc tgtaccgttc cgctcccgcc acgtcgttct cctcaaaccc
     6001 cgccttcgcg gccagatagg gataatggac ccggatcaac cgggagggat agtcgtcggg
     6061 cgaatgcggt ccccggccgc gccaaatata aaaatccgac ggccggcccg tctttttttc
     6121 ctgaaaaaga agtccgttga ggacgaaatc ccccggcggc aggttgtccc gcacgaacgc
     6181 catcaccgac aaaggccggt ccagcgtccg ccacagggcg tcctcccgtt cctgttgccg
     6241 gtgatatcgc cggtcccggt ggatctccag aatgtcctcc ccgtaaaaca gctcgaagag
     6301 cgtccccggc cgatagatcg ggcgcacgtc cgggcgctgt ctctccacca gcaaccggtt
     6361 catcaggcag gaggtgggtt cgtcgaacat cacccggtcg atcaacagca gcgaccgagg
     6421 ctccagcgag gaacagaggt tccgcccgaa atcgcggaac cccaggttcc accggaacca
     6481 cggatgcggc ctaaaaaaaa cctgttccac gcacaccgcg cccagaagca cgatcgccag
     6541 ccccgtgcgc cgtttcgaca gaaggcgttc cgtcagcaag gccgcgcccg cagccaaaaa
     6601 cagcatcggc aacaggagga accgttccat ccgccactgg gccagcgtat tgaacggcga
     6661 gaggttggaa aaaacataaa agaacgggcc cggcaggatc caaaaaagaa gccaggcccc
     6721 acgagagacg gaagacgtcg cgcggcggac gtcggccgcg gggtccggcc tcctcgagct
     6781 caaaagaaac cccaacaaac ccagcgccag gacaaacccg ccgcccgcgg cgacgttctg
     6841 acggaaaaaa cttttcaact gggcggcgac cgtccccgcc gtatggaaag gcaaggccgc
     6901 gggatggagt tccagccggc cgaattcttt ccgggtcaac acgtccacga accgccggag
     6961 cgtgtgcggg tcgccgaaat tcaccggcgg atgccgagcg gcgcgcaagg gcagatacag
     7021 atacacggaa agacccagga ggaagaacgc cgtcgcggcc gggagccaat cggtcgcgga
     7081 gcgcgccgcg cgtttcagat tctccgccgc gccggcgccc cgttcccaga agaaccgccc
     7141 ggcggcccac acaaaacccg gagccaggaa gacgagcgtc tgatgattgg ccaaaccgag
     7201 gccgtagaga aaacccccgc cggccagcca gcggggccgg agccggccgt cggacggaaa
     7261 cgcgcggacg aaaaaaagga tcaacagcgc ggccaacaga ttgttcaacg cgaagacttc
     7321 cgtgccctgg gcctggtccc acacgatggg cagggccgcg tagaccaacg ccccggccgc
     7381 cccggccacg gcgtttttca gaaggtcccg caaggccgaa aacaggacga cccccgtggc
     7441 ggcgctcagg aacgccgaaa acaggttcac gcgataggcc ggcccgcccc acgggagcac
     7501 gtttgaaaac agtttcccca tcagcgcgtg caggggatac cccggagcgt gcgaaacacc
     7561 cagcgccaac gcggaggtga tgagttcgcc gctgtccccg ccgtacaagg acggcatggc
     7621 gcccgtccca taggcggcga aagccgccag gaacagtccg gccgccgcaa acgaaccccc
     7681 ggtcttcgcc tcttccgtcg acaaggccat caaaagatct ccgcccaccg caggatccgt
     7741 tccggattcc aaaagagcga ccgggcgtaa aaagcgaggg agctctcccg gcggccgccc
     7801 tcgtggagca agaggccggc gttgaacccg ccggaggaca ccgttttcag aagggtgcgg
     7861 gtgtaataat cggcccgcag caccgcggcc gacgggagcc ggcggacata gaacccccac
     7921 aacgggagcg attcctccat ccggtccaga caggccgggg tgcgtttcgg ccattccaca
     7981 gcggccgcca gcccgtaggg gtggagcgac tccacgaatt tcggctggat gccctcggtg
     8041 aacaggccca cccggcccga ctggcgccga agcagttcca ttcccccgtc ctcctgcccc
     8101 gcgctgaaca gatccggaaa gcggcggcga tattgccgcc accgccacga cggagaggca
     8161 tccaccagaa gacgcacatc cgaccgggcg cccgccgcct gggaataggc ggcggaaaaa
     8221 acggaggtgt ccgagatcat caacagaacc gaggccggcg ggaccgtccg gaacaggttc
     8281 ctcccgtaat ccatcgagac caggttccac cgctgccgct ggctcaggac ggcgggaaca
     8341 ccccggccca cgacgaacag cgtcacggcg agcagtccga ggaccgccag aaaacgtctg
     8401 cgtttcaaga gagcctccaa aaaaaatccc atccccaacg ccgccgccag gccgggcagg
     8461 accagggccg gcgacagaac ggcctcggaa tgttccgccg gctgcaatcg cgccaacaac
     8521 gcgaacaccg ggccggaaaa aatccacagc aagagcaccg gggcgcccca cacctttctg
     8581 ttttcctttc ggaacagcca cacggcggcc gccaactgca gcaccaccgc ccacgccccc
     8641 caccggcggc cccacacacg gaaccaaaac gccaatccca tcgccggcga ggccgtggaa
     8701 tacagggacg aaagcgtgcc ggttccataa tcggcccggg tgaacatcgc ccaaaaaccg
     8761 ttccaggtgt ccggttcgcc ccacacctga accgcctccg cggcggcgcg gagcggtaac
     8821 aagagataaa cggccagccc cagcccggcg gcggccaaca cgacgcggac aaagcggggg
     8881 tgaaggacca ggcttccgcg ctcacgccac aacaataaaa ggaccgacgg ccccacggcc
     8941 aggagggtct gatggttccc catccccatc cctcccagaa gccccagacc cacccacccc
     9001 cgcgtgcgtg ccgacgggga aagagccggg ataaagaacg ctccccagag gaggagggcg
     9061 gcgaacagag cattgagccc atagatctcg gaaagaccgc tctgggccat gtgcggcgag
     9121 aaaaaaccga tgaacagcgc tcccagcaga gaggcggcga gaccgcaaaa ccggctcaag
     9181 ctcaagaaga cgagcaggac gaccccggcg ccgcaaaaag cggagacgat gttggcccga
     9241 taggcggggt tgccggacag gaagggttgg aaaaatttcg ccagaaggac gtaggacgga
     9301 tatcccggcg gatgcggcac gccgaacgac agggcggcgg cgctgaaatc gccggcgtca
     9361 cggaaagagg aaagggccgg gaacgacaga tacgcgtaaa agccgaaaaa aatcaggaac
     9421 aggctcgcgg ggaaaatttt tttcatgggt tcgggcggtc tcgagggcga aacaagcctc
     9481 ccctcggcct ctgccggtgg gattttaaca ttttctcccg tcagagattc caccaacaaa
     9541 aaaacccctt gttcaggggt ttccaacgga gaccggacgt gcgggacggg tcagggggcg
     9601 ctggtccgga gatggggctc gaaatggacc gattcggcgt cggcctgggc cggccgggac
     9661 aacaaatcct gcagctgctt gatgagtttc tggaaccgaa gcggcttctc aaaaaccatc
     9721 cgcgcgcctt tcaaaatacc ttcggtgagc gcgcccttgg cgaccaccga gatgatgacc
     9781 acggggatcc ggttggtgcg ctcgtctccc tggagacgac gcaggacttc gatgccgtcc
     9841 acccccggca tctcgatgtc cagggtgatg aggtcgggtt tccattccgc ggccagttcc
     9901 accgctttgg aactgtcggt gaggaatcgg gtgtccagcc cggcgtcacg gcaaaattcg
     9961 gcgatcagtt ccgccaggtt tttgtcgtcg tccaccacca gtatcttttt catctcgcgc
    10021 gatgatcctt tctctcggcg ctgttttgac gggcgaaaaa accggatccg ccccacaacg
    10081 gtttcccggc acaaaaacac ctcaaacgcg tggtccgccc tgaactctct cccttttcag
    10141 ggctgacaaa aacacggtaa cataagcccg ttaaggtcgg ctgacctggt tgtaaaaaat
    10201 ctgtaaaagt taccggaaat cagacgtttt ttggggggga agaggactga cggaccatga
    10261 gttgcaccgg atatttctcc tggaacacgg ccggcggttc cccgctctcg atctgttgga
    10321 tgatgcggcg cgtgccgtcg gccgccacca tcgaaatgtc gtatttcacc gtggtgagcg
    10381 gcggttccac caaacgggcc aggtccaggt cgcccgatcc gatgacggag acatcctgcg
    10441 ggatgcggag tcccgcttcc ttgatggcgc ggaaggcgcc ggaggccatc cagtcgctgc
    10501 ccgcgtagac ggcggttacc ttgttggcca agaggccgcg gcagacttct tgggccacgt
    10561 ccgccttgta gtggccgtcc accaccaacg tcggctcgaa ggggatcccc gcctcttcca
    10621 gggcgcggcg atacccgcgg aggcggtccc gcgccgagga aacggcggcc gacccggcga
    10681 tgtggccgat ccgccggtgg cccagatcga tcaggtgttt ggtggccatg tgggcgcccg
    10741 cctcgttgtc caccgccacg taggagagtt gggcgtcgtc cagatacccg cccaccatca
    10801 gaaaaggata ttgatacggc tcgatctccc gaaggaaatc gtccttcagc gtcgtgccca
    10861 gatagacgat gccgtccacg gaattctcct tgaggagccg cagatagaac cggcgggaga
    10921 ggaactgcgg cgtggcgatc tccaggagca ggcggtatcc ctgacgggag gtttccgaat
    10981 agaccccttt cagggcttcg gtgaaaaagg agtgatccac caccgcgccc aggtccggca
    11041 ggaccacgcc cagcgtgcgc gaacgctgtt tggcgaggtt gcgcgcgatg aggttgggcc
    11101 ggtagccgag cgccttgact acctccagga cgcgctgggt ggtgtcgggc gggatccgct
    11161 ggtctcccga aagcaccagg gacaccgtgg aaagggacac gccgcaatgc tcggccacgt
    11221 ccttcatggt gacgcggcga aggcccgatt tcgactgttt gttttcctga gaactcatgc
    11281 ggtcttcctc aatgtgaatg cgggggcggc cgtcggagcc ggtccgccac cctcaggcgg
    11341 gccagggcga gccgcagggc ggccatggtc tgttccgaac cgcccggttc cccggcgccg
    11401 ccgcgcaggt cggaacgggc cttcgatacg gcctgacggg cgcgctcggc gtcaatttcc
    11461 gattccagtt ccgcggtttc ggcgaacagc gacactttgc cgccgaaaac ttccacgtat
    11521 ccgccggaca ccgccagcag gcgtgtttcc tcgccctgcc ggatccgtac cacccccggc
    11581 gtcaactgcg ccaaaagcgg cgcatggccc ggcaagatcc ccaattggcc ctcgtgcgca
    11641 gggatgacca gggaatccac gttcccttcc cacgacacct tctcgggcgt aacgacgctg
    11701 aggggaaatg gccgggccat gaccgattat tttttcttgg ccgccggggc cggagcgccc
    11761 tgcttggcca tctgttcctg gatctgtctc tgtatctcgt tcgccacgtt ctccacggat
    11821 ggaggctccc ccgcctcctg gcccgcaaac ggaggaacgc cgccgggcat ccccggctga
    11881 ttgagatacc cttgacgttc cagttccgaa cggatgttcg cggtcgccag gattaatatc
    11941 acgaaggcga cgccgatcgc gccgaacacc gcccacgctt ttttcggcga aatcttgtgg
    12001 gtctccatgg acgcgatgat caaaaggaac acgccgtaga ggaaggccag ggcgttcagg
    12061 aacgggacga gccccagcac cgcccccaac accgcgagcg gcgagatgac cgcccagcac
    12121 cggaacgcgg tggcgtagtt ctccttggac cccatgaggt gccacagcac gaagagcacc
    12181 gccgacacca ggaaccccat ccccaacagg atgaacggcc cgaagacgag ccagacgatc
    12241 tccgccgtcc agccgagccg caccggcgag gtccgaagcc ggccgatgat cagttccaag
    12301 accgtggaca cgtacagcca gaacagcgca tagaggatcg gcgtggcgta tcccccctcc
    12361 ttggccagcg cggaaaagaa ctgtttcggc gccgtcacca aacgcaccgc ccaggcccac
    12421 aggctgttca aagacaatcc cctccccgcc acttctccgc tgattccctg atcgttcacg
    12481 atagtctccc tgttcgtttt ttaagattcc aacgactttg ctttttccac cacttcgtcg
    12541 atgccgcccg ccatgaaaaa cgcctgttcc ggcagatggt cccaccggcc ctccaccacc
    12601 tcgcggaaac tgcggaccgt ctccttgagc gggacgtacc gtccggcccg gccggtgaac
    12661 tcctcggcca cgtggaacgg ctgggaaagg aacttctgta tcttccgcgc gcgaagcacc
    12721 acggtcttgt cctcgtcgga aagttcgtcg atgcccagga tggcgatgat gtcctgcagg
    12781 tccttgtaac gctggaggat gcgctggacc gaacgggcca ccgcgtaatg ctcatcgccc
    12841 acgatcttgg gatcgaggat gcggctggtg gagtcgagcg ggtccaccgc cggatagatg
    12901 cccagctccg agatggggcg ggacaacacc gtggtggcgt ccaagtgggc gaaggtggtg
    12961 gccggcgcgg ggtccgtgag gtcgtccgcc ggcacgtaga tggcctgcac gctcgtcacc
    13021 gaccctttcg tggtggaagt gatccgttcc tgcagttccc ccatctccgt cccgagcgtg
    13081 ggctggtatc ccaccgccga cggcatgcgg ccgagcaggg cggacacctc cgagccggcc
    13141 tgggtgaacc ggaaaatatt gtcgatgaaa aggagcacgt cctgcccctc gacgtcgcgg
    13201 aaatattccg cctgcgtcag ggcggagagg cccacccgca accgcgagcc cggcggctcg
    13261 ttcatctggc cgtaaaccag cacggtcttg gagagcaccg tctggccgtc ggaaagtttc
    13321 gatttctgca tctccagcca gaggtcgttg ccctcgcggc tccgctctcc cacgccgccg
    13381 aacaccgaca ccccgccgtg ttgtttggcg acgttgttga tgagttccat gatcaccacg
    13441 gtcttgccca cgccggcgcc gccgaacagg cccaccttgc cgcccttcat gtacggctcc
    13501 aacagatcga tgaccttgat gcccgtttcg aacacctgcg gggtggtggt ctggtccaca
    13561 aaagccggcg gcgcgcggtg gatgggcagc cgcgtggaac ctttcagcgg gccgcggtgg
    13621 tccttgggtt cccccagaag gtccatcagc cggccgagac attccttgcc gacgggcacg
    13681 gagatggggg cgccggtgtc ttccgccgcc aggccgcgcc ggaggccgtc cgtggggccg
    13741 agcgccaccg cccgcacgat gttgtcgccc aaatgctggg ccacctcgac ggtgaggagg
    13801 cgccgttccc cgcccgattc gtgcggcacc cgcacggcgt tgtggatcgc cggcaaggac
    13861 cccggagaaa actccaggtc cagcaccgga ccgatgacct gaaccactct tccctgattc
    13921 acatgccctc ccttaaaatc actcgcgttc gatctcccgg ccgaacttcg ccgtcacgta
    13981 acggcgcagc cggagcttgt cgaaaaaacc gatgttctgg aaccggatcc cgtaattcat
    14041 ccgtcccccg cggctcctca tatagaccac tttgcccagg aactcgatgg ggacgttcac
    14101 cttcaaaaaa agcgtctctc cggagcggag cgggacgtgc ccctccaccg ccatgccgcc
    14161 catgctgaag ttacgcacct gggcgcggcc gcgggccgat tgtccgccca cgtcgtgcaa
    14221 ctccaccaac atgtccgccg ggatccggcg aaaccgacga cgttccattt tctgtccctc
    14281 gggcaaatcc cgtcctactg cagcgcctcg gcgcccccca ccagttcgga aatctctttg
    14341 gtgatcgcgg cctgccggag cttgttggca tagagcgtga ggctctcgat cagttccttg
    14401 gcgttgcggg tggcgttctc catggccgtc atgcgggccg ccagctcggc cgccgccgac
    14461 tccatcaacg ccctgaaaat ctgcgccttc agcgcgcggg ggaacaggga ctccagcagc
    14521 cgttcccggg cgggctcgta aaggaaatcc ggggccgcgg gccgcaccga ggccgggacc
    14581 ggcggcgtca acggcagcca aacggacctc accggcctct gctgcatcac gcttttgaat
    14641 tcgttgtaga tgaccgtcac gtcccgcgcc ggccggttga cgaagaaatc cgtcacgtcc
    14701 cggccgatga gctccgcgtg agcgtaggag agctggttga aaatgttgac gtattcccct
    14761 cggacggcca ctttcatgcg gcggaagaaa tcgcggccct tccgacccac acagaaaagt
    14821 tcgaccgtct ccccttcgtt ctcccgcaga aattccaaac ccctcttgat gaggttcgtg
    14881 ttgaaggaac cgcacagacc cttgtcggcg gtgatcaaca ccagtccccg cgggccggtc
    14941 tttctctccg ccaggagcgg gtgccccaac accgtccgca ccgccgcgcc ggactgtccc
    15001 ggttcggcca cggcggaaaa gatatccgtc agcagggtct ccatcttctg agcgaacggc
    15061 cgcgccgaga ggatgcgccc ctgggcccgc ctcagccggg ccgccgacac catcttcatg
    15121 gccttggtga tctgctgcgt cgacttgacg ctcttgacct tccgacgcag ttcccgcagc
    15181 gaggccatgg gccgttccgg tctatttcga gatcttcttg accgacttgg ccttcagagc
    15241 gtagccggaa taggtcttgt acgcgttcgg ggccttgtcc accgagagct gcccttcgat
    15301 caacagccac tggcccggat cggccaggta aacctccaat ttggtggaca tgtcctccgg
    15361 gtcgaaatac acgccgacgt tgttcatgat gtccttcggc ttgccggtga ccacgaaaaa
    15421 gtcgcgctcc ttgtccaccg gcgcggccag caccgcctcc acctgcacc
//
LOCUS       DAOTFD010000038        15031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2725_length_15031_cov_5.369591, whole genome shotgun sequence.
ACCESSION   DAOTFD010000038 DAOTFD010000000
VERSION     DAOTFD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_15031_cov_5.369591"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="P5079_03955"
     CDS             complement(<1..1407)
                     /locus_tag="P5079_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HRY29173.1"
                     /translation="MNYKKLWIAMGAVLVFSFSILGYYGREIYHQAPPIPNRVTTPDG
                     TVLMTGEDIRTGQQVWQSLGGQELGSVWGHGAYVAPDWSADWLHREAVWLLDRWAQAE
                     RGKPYAECDAEEQAVLRTRLTQLLRANTYDAATGNLTVSPDRAEAIAAVGKHYAGLFG
                     NDPALAELRNAYAIPADSLKDAERQGPLNAFFFWTSWACVTDRPGKNISYTNNWPPED
                     LVGNRPTGQIVVWSVLSFVFLLAGIGALTWANAVLRRRGEEPMAPPANDPLLALNPTP
                     SMKATLKYFWVVGALIVAQVGLGALTAHFAVEGTGFYGIPLAQWLPYAVTRTWHVQLG
                     IFWIATAWLATGLFVAPAVSGHEPKFQRAGVNFLFVCLLIIVAGSLAGQWFGVRQKMG
                     LTTNFWFGHQGYEYVDLGRFWQIFLFVGLVLWLLLMIRAMAPAFRKPQENRNLLALFV
                     IASAAIALFYGAGLMWGHG"
     gene            complement(1576..2853)
                     /locus_tag="P5079_03960"
     CDS             complement(1576..2853)
                     /locus_tag="P5079_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29174.1"
                     /translation="MRPTAPPPSLDVVLLNPPFYRFLGSHNDKIPLSLAYLSAFLSDA
                     GISHTVYNADHTGARGHWSLKWMFDHYETFVDGVNGKGSLYGEVLETVLSWSPRTVVI
                     MGGDPLIPTTEWGNPFIAAHFSRLLRKRGIFTVGLGPFYRLDARRFLPDFDCLFTGEP
                     SSEILGVLRKKRRGILPFSKMALDVPPNLAQLYPSDQKTDTAVTSLGCRYQCSFCLSG
                     QFYRSSTAPVRYVEEKTVTEDLRSRKEKSIYLLDLNFPFSPIGRLRALDREFKKNRLD
                     KEFTVDGRADCLNEEKVKWLRELNVKRVKLGIEAVDPKTLERYNKKINLLQTERAIRM
                     LKNHGIGVIFYLILGPETTEDTYLSTREFLRKWEPDYIVPNLLAYDLKRDYRYDTEFS
                     PLKLKEWGVPRTLYYKYLTLQKKTNPTVGKIID"
     gene            complement(2857..3597)
                     /locus_tag="P5079_03965"
     CDS             complement(2857..3597)
                     /locus_tag="P5079_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY29175.1"
                     /translation="MIASILLPTLNEKENIVLLIKKITGVLERGTFEILVIDDNSPDG
                     TFAAVVEHFRDFPEVQPLLRETEQGFRSALQAGIHRCRGNIVAWMDTDFSHPPELLPQ
                     MIRLIQQGQADAVVPSRFIPGSRDFTGRNASLPVKFQKMSSGLLNRVLKKLFGVEFND
                     WTSGYIAVRAPLIKDHALQGVYGEYFIEMLYSLFEKKARIVEVPYVSEPRRFGSSKTA
                     PSLPGLLKKGAGYLFMTFRLLKRHFVGK"
     gene            complement(3773..5161)
                     /locus_tag="P5079_03970"
     CDS             complement(3773..5161)
                     /locus_tag="P5079_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRY29176.1"
                     /translation="MTIKYLLKESDIPKQWYNVAADLPGGVLPPLGPDGKPVNPQALA
                     PVFPMNLIEQEVSTQRWIDIPEEVLKLLLQWRPTPLHRALNLEKALGTPAKIYFKNES
                     VSPAGSHKPNTAVAQAWYNKQFGIQRLCTETGAGQWGSALSFACAQVGLECKVFMVRV
                     SFDQKPYRKFMMEAWGGKCVPSPSTDTAFGRQVLAATPDTPGSLGIAISEAIEAAVSD
                     LSGKTRYSLGSVLNHVMLHQTIIGQEAQKQMEMAGDSPDVVIGCAGGGSNFAGLAFPY
                     VREKIHGKKIDIIPVEPASCPTLTKSPFSYDHGDTAKMTPLLAMHSLGHAFVPEAIHA
                     GGLRYHGMSPLVSQAVMDGLLRPLSIAQLECFEAAVLFARTEGIIPAPETSHAIAAAI
                     REAKKAKEEKKAKTILFNFSGHGLLDLSGYNKFLSGQLVDHELSDDDIAKSRKCMEGF
                     PSPKDVAARLKA"
     gene            7264..>15031
                     /locus_tag="P5079_03975"
     CDS             7264..>15031
                     /locus_tag="P5079_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29177.1"
                     /translation="MRNVPSFRKHPLRRTLALLTCTVFLWTNVVWGAVSSETIWGERR
                     AALRTAPGARGTNGSSAAPSTTTPTQWAQAAPAALSWESVRESLSSRPALPASPPQKF
                     SSLPELSTEKWLQPLAPFQGLIKEIHLSKNPGAPRVLHFQDVHAHLGAQESLSELLGF
                     LNSDYRMTVVGLEGASGPFRVQPYREFPDAAVRDKAAHQLLRDGMIGGPEFFAMTSAE
                     EVPLWGVEDNGLYLDHIRAFRQMLPRDAAAKSLWGGLQAAVDALAQKVCSPAQVRLSD
                     LSRRYVHGEMPLTDYVSELAGGRRLLPSAYPALALFQRASELEKTLDFSRVESERRRL
                     LEALAEKLDERALAALVAQSLAYRMGSVSYGEFHRFLEELCRSRGVPWEGCPEFRRYL
                     DYVLLADRIDGRKILPELEALETARWSDAAVLPDSFVLQRLQQDLRLVEKLFSQSLTS
                     REWDQYAKRRAAGLASLPETLAGLGGRAPSGSGAFGEYLAGYERFYRLAEERNSVLLK
                     QLLEKSEEAGRRLSAARASPAVAVLVTGGFHTDGLTRLMREKDVSYVVLSPKVDLGAE
                     DFGSPHGLDFFKRTPTDLEKLFLGEKLFLNTPRLTAQAVPEEYKPLSAVAERYFASLA
                     VALKFLEVSDADAQAAGRAMMQWTTEHFDSIDRLETWGDIEKVNGVLRVPLQINDRRV
                     EVLLRARGAGERAFAALREGRPVVHQGETSTGERFEILAVSPPVWENAVEGASAALAR
                     GREALRSAGWTPSLHGVQSWEPTLRLLDMSQEAHRRVALDIVRSLRPGVRSPRLLEKL
                     QALEEMLSQPEVFSAIPVAWWQDLAENGPLLLPYEELFERHEQVWRSLNFILTRMHPL
                     LPGEAYEKTVQMAQRAGVAWNLKGLRFVEQSPDDALGAPADRPLRWTKDGYVLIHAEE
                     LAKWEAEGKDVFKSLFLTLLHENHEFTAYQNKLKSGEEPEADDFDAAHRDALLRGFHP
                     DNPAPLSDILVSLPATARRPPLTHDFLQKLTEEVRLRWVEKDPAVLRAFLDLVANPLI
                     DDAAFPILAEFVATPGFDRAVVRIREESRKKRPEASGVLGELYHAARLKAMGYEIVSL
                     GFSVSENLDRRKTGLVEIDILARKDGVFYFVEAHHSLAGAAIGLGRGDFTALDKLAHL
                     KLKKDKFGRYKAGVMELRRVLKRAAGPRDADRRFRQALKRNTDHFFGGNKELAQKFYD
                     QVASDGPVPVVLTLTDSGGTAYSHEASNFENEWGKHLSVRGARLAGVSFPVLVRVFPF
                     QSLNAAEGPAPALSGLREAFSDDQKHLGDAVSARGAPSDKTSARSVLESVFGEHPAVY
                     ERLMELTLTRFAPNLKTYQKLLRDDILPDVFLQLFRVRAKTLNLPGLAEYARYVKKTD
                     AEKKWIADKLVLSAAKVQGNSEFFFRRAEWPLEESLPRLIESKIQNGQYRLRLKSFFS
                     AGGEDPYSLALAVRQALEDYYVAHKDAIPETDVDDWLNKWDVRIDALETSFGGLYMTH
                     LGRFDELDGAELPERFQRQFQTSYHKLEIGEEFRKWVVPQFVDFDDPAERAALNDGEA
                     DIVFLPRLPGALGDRPAVLEAAEGALAPRANGMFLFSTEETERELPLLALESDGRGGW
                     RSSLRTKRAVGKLTDALRSAPLLRREQAALMLGVAATPASRAGMESARTGASPRLDAI
                     LDWALRRTPPTAAVFFEEEPPAPAGEGTRALAVMDERYHIRKKYDDLIEKRRQDMREA
                     KKNLRKHQDALKRMMASPPAAPSSAEEKSAALESLRTQQFRVEGARLRVKNAINRYDA
                     ARRWREESAGSLSKTVSLFDRLAATPNAMQEMAIGLAEKYLQMPRDHIFFIDPQHPMA
                     EASGLEWDINREGHALGIHVVMNDGQEAGRYLFINAAVGDYFTLVRTIVHESWHLKGF
                     PKAPLNLPTGLMKMLEEGITELRTENTMSQILQDAQKSKDGTYQAFIESLDEEYRALL
                     KATKEEAPAGVTWDDKLDLVLNRSPYLDYETLVMDMVEAMDRETLTRLFEEGNLPGLQ
                     QRLGDKLPALERMSPYLLLPPNSPLRLFAAYAMQEILQGRFNDSRLAGWEAVLRSLAD
                     VNTYVEWASVSEDGSSPWMEDESFVSQMIAGIQTFAEEVYHGGLLEVWAREAARNVEA
                     GGEAISAALHGQMQERFVRTLLTGMAAQPGSMLEALSPEEAARRVAEEGVYLLQDPNR
                     TLSAEEAVRRIQEGSLLAFQNEKTVVWIPATAGRKSAQPMHQKLYDKARAGFTKAEKA
                     LNKKREKGEATESDLAEESQFLKKSFDETVVFWDRLQKAGTDVERIELFLTEGVPLLI
                     GLSNAETDNEAIVTAVQDAIRAAAYQIDSDFGQAVSRFVRLLAENDLDSTGDKIYAVF
                     RDMNSRFFLPNGFYVDVVVEAGLEEVTVIPYQLASAPRQIKLLPDKQAYSFYHVRHLR
                     ADSAQAVGESIGTVGGVKAYRDAVQETVVVFENEMLREAEEILHQVRGETAFSLFDKG
                     DEEIWAGFVKSVRGLEGASSEDKGFWLSLRDDPEIAELVRQAKLKDFSPQETVDAVAA
                     KNRLATVLHELRHALDELKDLFEALAKKPRKKYIAGEVSANLAEIALGPTP"
BASE COUNT         2812 a         4437 c         4913 g         2869 t
ORIGIN      
        1 gccgtggccc cacatcagcc cggcgccgta gaacagcgcg atggccgcgg aggcgatgac
       61 gaacagcgcc agcaggttcc ggttctcctg cggttttcgg aacgccggcg ccatcgcccg
      121 gatcatcagc aacagccaca acaccagtcc gacgaagagg aagatctgcc agaaccggcc
      181 caggtccacg tattcgtagc cctggtggcc gaaccagaag ttggtcgtca gccccatctt
      241 ctggcggacg ccgaaccact gtcccgccag cgatccggcg acgatgatga gcaggcacac
      301 gaagaggaag ttgaccccgg cccgctggaa cttcggctca tggccggaca cggccggcgc
      361 cacgaaaaga cccgtggcca gccacgctgt ggcgatccag aagatgccga gctgcacgtg
      421 ccaggtgcgg gtcaccgcgt aaggcagcca ttgggccagc gggatcccgt agaagccggt
      481 gccttccacc gcgaagtggg cggtcagcgc gcccagcccc acctgggcca cgatcaacgc
      541 ccccaccacc caaaaatatt tcaaggtcgc cttcatcgag ggcgtggggt tcagcgccaa
      601 aaggggatcg ttcgccggcg gcgccatcgg ttcttccccc cggcggcgga ggaccgcgtt
      661 cgcccacgtc agcgcgccga tgcccgccag aaggaaaacg aaactcagca ccgaccacac
      721 cacgatctgc cccgtcggcc ggttgcccac caggtcttcc ggaggccagt tgttggtgta
      781 ggagatgttc ttgccgggcc ggtccgtgac gcaggcccac gacgtccaga aaaagaaagc
      841 gttcaggggc ccctgtcgtt ccgcgtcttt gaggctgtcg gccggaatgg cataggcgtt
      901 tcggagttcc gccagcgccg ggtcgttccc gaaaaggccg gcgtaatgtt tccccaccgc
      961 cgcgatggct tccgcgcggt ccggggagac cgtcaggttc ccggtggcgg catcataggt
     1021 attggcccgg aggagctgcg tcaggcgtgt tcgcaggacg gcctgttctt ccgcgtcgca
     1081 ttcggcatag ggtttgccgc gttcggcctg ggcccagcgg tccagcagcc agaccgcttc
     1141 ccggtgcagc caatccgcgg accagtccgg cgccacgtac gcgccgtggc cccacacgct
     1201 gcccaactcc tgtccgccca gagactgcca tacctgttgg ccggtgcgga tgtcttcgcc
     1261 cgtcatcagc acggttccgt cgggcgtcgt cacgcggttc ggaatcggcg gtgcctgatg
     1321 gtagatttcc cggccgtaat agccgaggat ggaaaaagaa aagaccaaga ccgcccccat
     1381 ggcgatccac agttttttgt aattcatgaa aacctccgtc gatttttaaa aagcacgatt
     1441 aacaggaaac tcccgcctga agcttccccg ggaccggatg aatctttccg atgaggagaa
     1501 cgagaggctc tattatagga aacaggggat tagtttagag gaaaaaaggg agcttcttta
     1561 tgacgggggt catgatcaat cgattatttt cccaacggtc gggtttgttt ttttctgcag
     1621 cgtcagatat ttgtagtaaa gagtccgggg gaccccccac tcctttaatt tgagtggaga
     1681 gaattccgtg tcgtagcggt agtccctttt caagtcatac gccaggaggt tgggaacgat
     1741 gtagtccggt tcccattttc ttaagaactc gcgggtcgaa agatacgtgt cctcggtggt
     1801 ctccgggccc aggatcaggt agaagatcac cccgatcccg tggtttttca gcatccggat
     1861 cgccctttcg gtctggagca agttgatttt tttattatag cgctccagcg ttttggggtc
     1921 gacggcttcg attccgagtt tcacccgctt cacgtttaat tcgcggagcc acttgacctt
     1981 ttcctcattc agacagtccg cgcgtccgtc gacggtaaat tccttgtcaa ggcggttttt
     2041 tttgaattca cggtccaacg cccggagccg cccgatgggg ctgaacggaa aattcaggtc
     2101 caggagataa atgctcttct cctttcgtga acggaggtct tcggtcaccg ttttctcctc
     2161 cacatagcgg acaggggccg tcgacgaccg gtagaattgc ccgctcagac agaaagagca
     2221 ttgataccgg catcccaagc ttgtcaccgc cgtgtccgtt ttttgatcgg aaggatagag
     2281 ctgggccaga ttcggcggca catccaaggc cattttcgaa aacgggagga taccccgtcg
     2341 cttttttcga aggacgccga gtatttcgga agagggttcc cccgtgaaga ggcaatcgaa
     2401 atcgggaagg aatctccggg catccaggcg gtagaagggg cccagcccga cggtgaaaat
     2461 accgcgtttc cgcagaagtc tggaaaaatg ggccgcaatg aacggattcc cccattccgt
     2521 cgtggggatc agcgggtctc ctcccatgat gacgacggtg cgggggctcc aggacagaac
     2581 ggtttccagg acctcgccgt agaggctccc ctttccgttc accccgtcca cgaacgtttc
     2641 gtaatggtcg aacatccatt ttaagctcca gtgccctcgg gcgccggtgt ggtcggcatt
     2701 atagaccgtg tgggatatgc ccgcatcgct caaaaaggcg gacaggtaag ccagcgacag
     2761 cggtattttg tcgttgtggc tgcccaggaa acggtaaaag ggagggttca ataaaacgac
     2821 gtccaacgag gggggagggg cggtcgggcg catcggttat tttccgacga agtgacgttt
     2881 gagaagacgg aatgtcataa agagatagcc cgcccctttt ttcagcaggc ccggcaggga
     2941 aggggccgtt ttcgacgacc cgaatcgcct cggttccgag acgtagggga cctccacgat
     3001 ccgcgccttt ttttcaaaaa gagaatagag catttcgatg aaatattcgc cgtagacccc
     3061 ctggagcgca tggtccttta tcaaaggagc gcgaacggcg atgtaaccgc tcgtccaatc
     3121 gttgaattcg accccgaaga gttttttcaa gacccggttt aagaggccgc tcgacatttt
     3181 ttggaatttg acggggagcg aggcgtttcg gccggtgaaa tcccggcttc ccgggataaa
     3241 acgggacgga accacggcat cggcctgccc ctgttggatg agtcggatca tttggggcaa
     3301 aagttccgga gggtgggaga agtccgtgtc catccaagcg acgatgtttc cccggcaccg
     3361 atggattccg gcctgcaggg cgcttcggaa cccctgttcc gtttcgcgga ggagcggttg
     3421 gacctcggga aagtccctga aatgttcgac gacggccgcg aacgtcccgt cgggggagtt
     3481 gtcgtcgatc acgaggatct caaacgtccc tctttcgagg acccccgtga ttttttttat
     3541 caataaaacg atattttctt tttcgttgag agtggggagg aggatggagg cgatcatggg
     3601 cgatctttca gtccgttctg tgatttaaac ggcgtcggca tgggcccttt ttgggggggt
     3661 ccgtcgacga cgcggcgctc cgggataaaa gacggggagc tttgcaaacg ggcggaacgt
     3721 tcccgtgtga aggcctctcg cggtccgcgg cggcgggcgg gagtcggaag aattacgctt
     3781 tcagtcgggc ggccacgtct ttcggcgagg ggaatccctc catgcatttc cgcgacttgg
     3841 cgatgtcgtc gtcggaaagt tcatggtcca ccaactgacc ggaaaggaat ttgttgtagc
     3901 cgctcaggtc cagcagacca tggccgctga agttgaagag aatggtcttg gccttcttct
     3961 cctccttggc ttttttcgcc tcgcggatgg cggcggcgat ggcgtggctg gtctccgggg
     4021 ccgggatgat gccttcggtc cgggcgaaga ggaccgccgc ctcgaaacat tccaactggg
     4081 cgatggacag gggccgcaac aagccgtcca tcaccgcctg tgacaccagc gggctcatgc
     4141 cgtgatagcg caggccgccg gcgtggatgg cctcgggcac aaaggcgtgt cccagcgagt
     4201 gcatcgctag gagaggggtc atcttcgcgg tgtctccgtg gtcgtagctg aacggcgact
     4261 tggtcagcgt ggggcaggaa gccggctcca ccgggatgat gtcgatcttc ttgccgtgga
     4321 tcttttcccg cacgtagggg aacgccagtc cggcgaagtt gctccccccg cccgcacagc
     4381 cgatcaccac gtcgggggag tcccccgcca tctccatctg tttctgcgcc tcctggccga
     4441 tgatggtctg atgcagcatc acgtggttca gaacggaacc gagggagtaa cgcgtcttgc
     4501 cggacagatc gctcaccgcc gcctcgatgg cttcggagat ggcgatgccc aggctgccgg
     4561 gggtgtccgg ggtggcggcc agcacctggc ggccgaaagc cgtgtccgtg gaggggctgg
     4621 gcacgcattt gccgccccag gcctccatca tgaacttgcg gtagggtttt tggtcgaacg
     4681 agacccgcac catgaacacc ttgcattcca gtcccacctg ggcgcaggcg aaggagagcg
     4741 cgctgcccca ttggcccgcc ccggtctcgg tgcacaggcg ttggatgccg aactgtttgt
     4801 tgtaccacgc ttgcgccacc gcggtgttgg gtttatgact gccggccgga gagacgcttt
     4861 cgtttttgaa atagatcttc gccggcgtgc ccagggcttt ttccaaattc agcgcgcggt
     4921 ggagcggggt cggcctccat tgcaacagca gcttcaggac ctcttccggg atgtcgatcc
     4981 accgttgggt ggagacctcc tgctcgatca ggttcatcgg gaagaccggg gccagcgcct
     5041 gcgggttcac gggttttccg tcgggaccca gcggcggcag cacgccgccc ggcagatccg
     5101 ccgcgacgtt gtaccactgt ttggggatgt cggattcttt caaaagatat ttgatggtca
     5161 tgggcgatgt ccttgtacgg gaatttgata tggtcccccg gccgctgagg cgccgaagga
     5221 cctgactatt aaaccaccac aacccacgcc tcgtcaacag aggagttggc gccgatgcgg
     5281 ccgggatcgg gggatcgcgc caccgccgcc gtcggacgcc cccggccggt ggtctgattg
     5341 acataattga ctaaataatg agcgtgggcg tccgacggcc ggcgctttcc ccggcagccc
     5401 cccgcccgag ttccctctct ggagcatggg aaggaacggg ggatcttgac ggccgacgaa
     5461 tccgcgtgcc cggtggttcc ggaacccttg attgaaaccg tgcgggtgct cggtcgtgtg
     5521 gtgggggcgg ccgctctcca cgttcgcaca cccgggtcgg tggatcttgt cacgggacaa
     5581 gtggattgat cggacagggg cccggccgag gacgcgtcat ccctgggcgt gggaacgcat
     5641 cgcggtccag cggcccgcgg acaagaaatg acgggatccg gggaagtcgc gtggaggcca
     5701 cggggagtct ttgcgagacg ttcttgcgga gggcgttccg cttccgcggt ttcatcgggg
     5761 gttccgcggt cggggactgg gagaaccgcg gggaaggaaa tggaggacga ggccccgctc
     5821 ttcggtcaga gattcccggc cgggccctgg gaagacgaga aaggcacacc ttcctttggc
     5881 cgcgtgtcgc gcgcggccgc gggctcttcg ctgggcgagg ggcgggaaga gagcgcgaga
     5941 ggttacgcct ccggatctcg cttttcgaag cgaggtttgg gaagccggcg ggggctggtg
     6001 ggccgcatcg aagcagggtc tgtttggagg atttgcggac ccccccgccc ttcaagtccg
     6061 cggcttcaga ggccgagaaa cgcggtttcc cctctcccgg ggacgaaccg gggattcgtg
     6121 acggccgagg tccgctggcg gtgcccggca atcattccaa ttcccgcccg ctcctcgccc
     6181 ccgccgccct ggaactgttg ggagagagag cggaagaacc ctggtgggcg gcgggggcca
     6241 tccgaaaaaa cggggaaccg cgggactcga gttgccgccg ccggcccgcc gcggggtgtc
     6301 ggctgttttc ggatggggcg ggtgggggcg aattttttgt tccccctccc cgctcgggcc
     6361 gctcccgcgg cagagaggct gtcggccctt cctctataag gcccccgaac aagtcgcccc
     6421 ggacggggaa gggaagccgc gcgttgtttc acgtgaaaca acgttcgcgt cgggacgtgt
     6481 cccccgtccg cccgggcgcg cccctggccg gccaagacaa taaaaatgcc ggcgcacaag
     6541 cgcccagacc ggtcctccgc tctgttgggg agggggtgcg tcgccgccgc ggacagagag
     6601 aggcgtgaga gcaaatcccc cccccattct ctttcaacgg ggggcataga atagaaaaga
     6661 gagcggaggt ttgccggggg gcggaagaaa gcggcgtgct tttgaaaatg gaaacgcgga
     6721 gggggacgga gggaggggag gtggcgccgt aaggaaggag gccggtctcc cagcgccggg
     6781 ccgccggcct cgccgaaccg tggtttcggg ctttctccgg aggggtcgtg gggggttgaa
     6841 gcggccctgg tttgtttcac gtgaaacaaa cggacggaag gccgggatgg ggcgaccttt
     6901 ttcgcgcccg ccggctctca tggttttttt gggggtcctg tttttccccg tttttcagaa
     6961 ttttcggtga attcggagat tttcgagcgg ggcgcaattg aaatttcgta aaacaaataa
     7021 aaaatagtaa aaattttaat aaaaaaaaca aacacatcgc aaaatcgatg tgaagaacta
     7081 cgaaaattca ataaaagtaa atcgcttgaa tattttagaa aaacacaaaa aaatcgatgt
     7141 tttccctgtc tttaggaaga ttcagaaaca gagagggggg gcattaaata gagtaaagtt
     7201 acatattgtt tacaatttgt tatctacttc tttaacgggg taccggtaca ctaggaccca
     7261 cgcatgcgaa acgttccgtc ttttcgaaaa catccgcttc gtcgcaccct ggcgctgctc
     7321 acgtgtacgg tctttctatg gacgaacgtg gtgtggggcg ccgtgtcgtc ggaaaccatt
     7381 tggggggaac gccgcgccgc cctgcggacc gcccccggcg cccgcggcac gaacgggtcc
     7441 tccgccgcgc cgtcgacgac aaccccgacc cagtgggcgc aggccgcccc cgccgcgctc
     7501 tcctgggaat ccgtccggga gtccctctcc agccgccccg cccttcccgc ctccccgccg
     7561 cagaagtttt ccagtttgcc cgaactatcg acggaaaaat ggctccaacc cctcgccccc
     7621 tttcaggggc tgatcaaaga aatccatctt tctaaaaatc ccggcgcccc gcgcgtgctg
     7681 cattttcagg acgtgcacgc ccacctgggc gcccaggaaa gtttgtccga actcctcggg
     7741 ttcctgaaca gcgattatcg gatgaccgtg gtcgggctgg aaggcgcctc gggcccgttc
     7801 cgggttcagc cctaccggga attccccgac gccgccgtcc gtgacaaagc cgcccatcaa
     7861 ctccttcggg acgggatgat cggcggcccc gaattttttg ccatgacctc cgccgaggag
     7921 gtccccctgt ggggcgtgga ggacaacgga ctttatttgg accatatccg cgccttccgg
     7981 cagatgcttc cccgcgatgc cgcggccaag tcgttgtggg gcgggctgca agccgcggtc
     8041 gatgcgctgg cgcagaaggt ctgctccccg gcccaggtcc ggctgagcga tctctcccgt
     8101 cgctatgttc acggggagat gccgctgacc gactatgtgt ccgaactggc gggggggaga
     8161 cggctcctgc cgagcgccta tccggccctg gcgcttttcc agcgggcgtc cgagttggaa
     8221 aagaccctgg atttttcccg ggtggaatcg gaacgccgcc ggctgctgga ggccctcgcg
     8281 gaaaaattgg atgagcgggc cctcgccgcc ctggtggcgc aaagtctggc ctaccggatg
     8341 ggctcggtct cctacgggga gtttcaccgt ttcttggagg agttgtgccg gtcgcgcggc
     8401 gtgccgtggg agggctgtcc ggagttccgg cgttatctgg attacgtgct tttggcggac
     8461 cggatcgacg gccggaagat tctgccggag ttggaggcgc tggagaccgc ccgttggtcg
     8521 gacgccgccg tcctccccga ttcgttcgtc ctccagcggc ttcaacagga cctgcggctg
     8581 gtggagaaac tgttctctca atcgctgacc agccgggagt gggaccaata cgcgaaacgg
     8641 cgcgccgccg ggctggcgtc cttgccggag accctggccg gcctcggggg ccgggcgcct
     8701 tcgggatccg gggccttcgg ggaatatttg gccggttacg agcgtttcta ccggctggcg
     8761 gaagagcgca acagcgtcct gctgaaacaa ttgttggaga agtcggaaga ggctggccgg
     8821 cggctttcgg ccgcgcgcgc ctcgcccgcg gtggcggtgc tggtgaccgg cgggttccat
     8881 accgacggcc tcacgcgttt gatgcgcgag aaagacgtgt cctacgtggt gttgtcgccc
     8941 aaggtggatc tcggcgcgga ggatttcggt tcgccgcacg gtctggattt ttttaaacgg
     9001 acccccacgg acctggaaaa gcttttcttg ggagagaaac tcttcctgaa cacgccgcgc
     9061 ctcacggccc aggcggtgcc ggaggaatac aagccgttgt ccgccgtggc ggaacgctat
     9121 ttcgcttccc tggccgtggc cttgaaattc ctggaggtgt ccgacgccga tgcccaggcg
     9181 gccggccggg cgatgatgca atggacgacg gaacacttcg actccatcga ccggctggag
     9241 acgtgggggg acattgagaa agtgaacggc gtcctccgcg tgccgctcca gatcaacgac
     9301 cggcgggtgg aggtccttct gcgcgcccga ggggcggggg aacgggcgtt cgcggcgttg
     9361 cgtgagggac ggccggtggt gcaccagggg gaaacatcca ccggcgagcg tttcgagata
     9421 ttggcggtgt ctcctccggt ttgggagaac gcggtcgaag gcgcttccgc cgccctggcc
     9481 cgcggccggg aggccctccg ttcggcgggg tggacccctt cgctccacgg cgttcagagc
     9541 tgggaaccga ccttgcgact tttggatatg tctcaggagg cccaccggcg cgtggcgctc
     9601 gacatcgtgc gttcgcttcg gccgggcgtc cgttcgccgc ggttgttgga aaaattacag
     9661 gcgttggagg aaatgctgtc tcagcccgag gttttctcgg cgatcccggt ggcctggtgg
     9721 caggatttgg cggagaacgg ccccctgctt ttaccctacg aggagctttt cgaaagacat
     9781 gagcaggtct ggcggagttt gaatttcatc ctcacccgga tgcacccgtt gcttccgggg
     9841 gaggcgtacg agaaaaccgt ccagatggcg cagagggcgg gcgtggcgtg gaacctgaaa
     9901 ggcctgcggt tcgtggaaca atcgcccgac gacgctctgg gggccccggc ggaccggccg
     9961 ttgcgatgga cgaaagacgg ctacgtgttg atccatgcgg aggaactggc caaatgggag
    10021 gcggagggga aagacgtttt caaatcgctg ttccttactc ttctccacga gaatcacgag
    10081 ttcaccgcct atcagaataa attaaagtcc ggcgaagagc ccgaggcgga cgatttcgac
    10141 gccgcgcacc gggacgcgct cctccgcggg ttccatcccg acaacccggc ccccttgtcc
    10201 gacattctcg tctccctccc ggccaccgcc cggcggccgc cgctgaccca cgatttttta
    10261 cagaagctga ccgaagaggt ccgtcttcgc tgggtcgaaa aagacccggc ggtgctgcgg
    10321 gcgtttttgg acctcgtggc caacccgctg atcgacgacg ccgcgttccc gatcctggcc
    10381 gagttcgtgg ccacgccggg gtttgaccgg gccgtcgtcc ggatccggga ggaatctcgg
    10441 aagaaacgtc cggaagccag cggcgtcctg ggggagctct atcacgccgc ccgcctgaag
    10501 gcgatgggat atgagatcgt cagcctgggt ttcagcgtct cggagaacct cgaccggcgc
    10561 aagaccgggc tggtggagat cgacatcctg gcccgcaagg acggcgtgtt ctattttgtg
    10621 gaagcgcacc attcgttggc gggggccgcc atcgggctcg gccgcgggga tttcaccgcg
    10681 ctcgacaaac tggcgcattt aaaactgaag aaggataaat tcgggcgcta taaggcgggg
    10741 gtcatggaac tgcgccgggt cctcaagcgg gccgccggcc cgcgcgacgc cgaccgaagg
    10801 ttccggcagg ccctcaagcg gaacacggac catttcttcg gcggcaataa ggagttggcg
    10861 cagaaattct acgaccaggt ggcgtccgac ggtcccgtgc cggtcgtcct cacgctgacg
    10921 gacagcggcg gcacggcgta ttcgcacgaa gcgtcgaatt tcgaaaacga atgggggaaa
    10981 catctcagcg tccgcggggc ccgcctcgcc ggcgtttctt tccccgtgct cgtgcgggtg
    11041 ttcccgtttc aatcgctgaa cgccgcggag gggccggccc cggcgctgag cggattgcgg
    11101 gaagcgtttt ccgacgatca aaagcatctc ggagacgccg tttcggcgcg tggcgcgccg
    11161 agcgataaaa cctccgcccg gtcggtcctg gagagcgtct tcggtgaaca tcccgcggtg
    11221 tacgagcgac tcatggaact caccctcacc cggttcgccc ccaatctcaa gacctatcaa
    11281 aagcttttga gggacgacat cctgccggac gttttccttc aactcttccg cgtccgcgcg
    11341 aagacgttga acctgcccgg tctggccgaa tacgcgcgct acgtcaaaaa gaccgacgcc
    11401 gaaaaaaaat ggatcgcgga taagctggtg ttgtccgccg ccaaagtgca gggcaattcg
    11461 gagtttttct tccgacgagc ggaatggcct cttgaagaaa gccttccgcg gctgatcgag
    11521 tccaagatcc agaacggcca ataccgcctg cgtttgaaat ccttcttctc ggccggcggg
    11581 gaggacccct attcgttggc gctggcggtg cgccaggccc tggaagatta ttacgttgcg
    11641 cacaaggacg ccatcccgga aaccgacgtg gacgattggc tgaataagtg ggacgtgcgc
    11701 atcgatgcgc tggagacttc ctttggcggt ctctacatga cccacctcgg acggtttgac
    11761 gaattggacg gggccgagct gccggaacgt tttcaacgcc agttccaaac ctcctaccat
    11821 aaactggaga tcggcgagga gtttcggaaa tgggtggtcc cgcagttcgt ggacttcgac
    11881 gacccggcgg aacgggcggc cctgaacgac ggcgaggcgg acatcgtttt cctgccgcgt
    11941 cttccggggg ccctggggga ccggccggcc gtcctggagg ccgccgaggg ggctctggcg
    12001 cctcgggcga acgggatgtt tttattttcc accgaggaga ccgaaaggga gttgcccctg
    12061 ctcgcgttgg agtccgacgg gcggggcggc tggcggagtt cgctgaggac gaagcgtgcg
    12121 gtggggaaac tcaccgatgc cctgcggtcc gcccccctgc tccggcggga acaggcggcg
    12181 ctgatgctgg gcgtggcggc gacgccggcg tctcgagcgg gcatggagtc ggcccgcacg
    12241 ggcgcctccc cccgattgga tgcgattttg gattgggccc tccggcggac gccgccgacc
    12301 gccgcggttt ttttcgaaga ggagccgccc gcgccggccg gtgaagggac ccgcgcgctc
    12361 gccgtgatgg atgagaggta ccacatccgg aaaaaatacg acgaccttat cgaaaaacga
    12421 cggcaggaca tgcgggaggc caagaagaat ctacggaaac accaggacgc cctcaagcgg
    12481 atgatggcgt cgccgccggc cgcgccctcg tcggcggaag agaaatccgc cgccctggag
    12541 tccctgcgga cccagcaatt ccgcgtggaa ggagcccggc tgcgcgtgaa aaacgccatc
    12601 aaccgttacg acgcggcgcg gcgttggcgc gaggaaagcg ccggcagtct ttcgaagacc
    12661 gtttcccttt tcgaccggct ggccgccacg cccaacgcca tgcaggaaat ggcgatcggc
    12721 ttggcggaaa aatatctaca gatgccgcga gaccacattt tcttcatcga tccgcagcat
    12781 cccatggccg aggcctccgg cttggagtgg gacatcaacc gcgagggaca tgccttgggg
    12841 atccatgtgg tgatgaacga cggccaggag gccggacggt acctcttcat caacgcggcc
    12901 gtcggcgact actttaccct ggtcaggacc atcgtgcatg agtcctggca cctgaagggg
    12961 ttccccaaag cgccgctcaa cctccccacc gggttgatga aaatgctgga ggaggggatc
    13021 accgaactcc ggacggaaaa caccatgtcg cagatcctcc aggacgcgca aaaatcaaag
    13081 gacgggacct accaggcctt catcgagtcg ctcgacgagg aataccgggc cctcctcaag
    13141 gcgaccaagg aagaagcccc ggccggcgtg acctgggacg acaagttgga ccttgttttg
    13201 aaccgttcgc cgtatctcga ttacgagacg ctggtgatgg atatggtgga agcgatggac
    13261 cgggaaacct tgacgcgttt gttcgaagag gggaaccttc cggggctcca acagcggctg
    13321 ggagacaaac ttccggcgct ggaaaggatg tcgccgtatc ttttgctgcc gccgaattcg
    13381 ccgctgcgcc tgttcgccgc ctacgccatg caggagatcc tgcagggccg gttcaacgac
    13441 tcccggttgg cggggtggga ggcggtgttg cggtcgctgg cggacgtcaa tacctatgtg
    13501 gagtgggcct ccgtttccga ggacgggtcc tccccgtgga tggaggacga aagtttcgtt
    13561 tcccagatga tcgccggcat ccagacgttc gcggaggagg tctaccacgg cgggcttttg
    13621 gaggtctggg cccgggaggc cgcccggaac gtggaggcgg gaggggaagc gatttcggcg
    13681 gcgctgcacg ggcagatgca ggagcggttc gtccgaaccc tcctgacggg catggccgcg
    13741 caacccggtt cgatgcttga ggcgctttcg ccggaagagg ctgcgcggcg ggtggcggag
    13801 gagggggtgt atcttttgca ggacccgaac cgcacgctct ccgcggagga ggcggtccgg
    13861 cggatacagg aaggcagtct gttggctttt cagaacgaaa agaccgttgt ctggatcccg
    13921 gccaccgcgg gccggaagtc cgcgcagccc atgcaccaga aactctacga caaggcccgc
    13981 gccggtttta ccaaagcgga aaaagcattg aacaaaaaga gggaaaaggg agaggcgacg
    14041 gaatcggacc tggccgagga gtctcagttc ttgaagaagt ccttcgatga gacggtcgtt
    14101 ttttgggacc ggctccaaaa ggccggaacg gacgttgagc gtatcgagct cttcctgacg
    14161 gagggggtgc ccctcctgat cggcctttcc aatgccgaga cggacaatga ggcgattgtg
    14221 acggcggtcc aggacgcgat ccgtgccgcc gcctaccaga tcgattccga tttcggtcag
    14281 gcggtttccc ggttcgtccg gctcctcgcc gagaacgacc tggattccac cggggacaag
    14341 atctacgcgg tgtttcgcga catgaacagc cgtttcttcc ttcccaacgg gttctatgtg
    14401 gatgtcgtcg tggaggccgg gctcgaggaa gtgacggtca tcccgtatca actggcgtcg
    14461 gcgccccggc agatcaaact cctgcccgat aaacaggcct attcttttta ccatgttcga
    14521 caccttcggg ccgacagcgc ccaagccgtc ggggagtcga tcggcaccgt gggaggggtc
    14581 aaggcctatc gggacgccgt ccaggaaacc gtcgtggttt ttgaaaatga gatgttgcgg
    14641 gaggcggagg agatcctgca ccaggtccgc ggcgagaccg cgttctccct ttttgacaaa
    14701 ggagacgagg agatctgggc cggtttcgtc aagtcggtgc gcgggctgga aggcgcctcc
    14761 tcggaggaca aggggttctg gctgagtctt cgggacgacc cggagatcgc ggaattggtc
    14821 cggcaggcca agctcaagga tttttcgccg caggagacgg tcgacgcggt ggccgccaaa
    14881 aacaggctcg ccacggtgct gcacgaactc cgccacgcgc tggacgagct gaaggatctt
    14941 ttcgaggccc tcgccaagaa accgcgcaaa aaatacatcg ccggggaagt ctccgccaac
    15001 ctggccgaga tcgcccttgg gccgaccccc t
//
LOCUS       DAOTFD010000039        14990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2737_length_14990_cov_4.193371, whole genome shotgun sequence.
ACCESSION   DAOTFD010000039 DAOTFD010000000
VERSION     DAOTFD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_14990_cov_4.193371"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /gene="pilQ"
                     /locus_tag="P5079_03980"
     CDS             <1..1668
                     /gene="pilQ"
                     /locus_tag="P5079_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRY29178.1"
                     /translation="RLVIELKEAVAQPYEAAPEDAVLKRIRCAQFQTSPKKIARVVLD
                     LSQHTSYETVREGNDLLLRFTGTTAAGAGAAPTAQKNTAAADRSTASPAMESVPPHKS
                     PLPEKTVSGETQASVGAAAATPGASKTPAKNAKDLLASLPKHPVTIDFEEADIRDVIR
                     VLSEMCGINMIQSPDLRGMVTIHLDQVPFNEAFNTILTMYGLVAQQMGENILRILTPE
                     ALSVDRSRAVTTYKTFTLNYANASDILQHLASVRISPMGRATVDERTNSLIVTDTPEG
                     IAAAERLIAELDEKPQQVMIEARMVEVTLTNSLDLGIQWEYAKESKTATNQTIIGQRN
                     VEAGGTTPTEGKASLVTSAGVESAFASGSRGTGVSLPGPQSTAITFGFINNTNLLTAT
                     LNALATSGQTKILSSPKVITVNNKQAKIQVGSQIPFSITTVSGTGIATQSFQMVDVGI
                     ILLVTPTINADNRIRLKVQPTVSTPGNVTAAGPEISTRNADTEVIIQDGETLVIAGLI
                     DEQMRESASKVPLLGDIPVLGVFFRKSSDSKQRTELLIFVTPKIIRD"
     gene            1687..2319
                     /locus_tag="P5079_03985"
     CDS             1687..2319
                     /locus_tag="P5079_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29179.1"
                     /translation="MNAPADGAKDSPSLTALLREAENYRQQKIWEAAIPSYQKALKLE
                     PGHVRSHLGLGHVYAAKGQEAGSRGFLLLSAEHFRKALATDPNCREAHESLLAVTAKM
                     GQWDELLQDYKARLVKDPENPSLKDAMKKIQAAFLLRTTPSAAPPKPQGLLWTFFNVG
                     LPLLGFVSFFLALIFRIQNIIPSWTTLYVGFLRMGTFFFVVYFLLRLFLK"
     gene            2332..4380
                     /locus_tag="P5079_03990"
     CDS             2332..4380
                     /locus_tag="P5079_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY29180.1"
                     /translation="MTEETLKEPRKDGAVAALSLVLFAAAFFAHGGKIVALWSPVSAG
                     LLLTAFLLLRRRPVFLLPSFLPGVLGFLGWGFLATLSSPSPLTSLWTMSKIGSVAAFG
                     VVAWFCWGEKANKIFRGLVMAAALAQSLLVFSSPLFGRPILLLPGNPHYMGLWMTVGC
                     LLGLNALVAPDISKRLRWTFAGITVFLAAATFWLPSRAGTIALVVGASFFLGRRFGRK
                     ALLSWLLFVLLLAWLFVGSLERLLTRSGDPLAWKRLDIWRAAVTGLSIHPYVGWGPGQ
                     FETLYQGCRPPQTDLPVWYGVTTAFAHNDYLQLATECGLPAAVFLLWGLACFFFYNRP
                     RDGPALAAHAAVLAIGVFCLFNFPMFLPVNGLLAAGLLVQGETRTEKFRLALSPRLGS
                     FFRRTILTLTALFAVSAGAVFLIELPATAGVRSLSRYRLWNPASLLEQADAFLHSADK
                     KDVHPESAERLLRRIVLFDRSNAKAWRDLSHLLLMHRADPDLETARRALAKALFWHPT
                     EATWYLELSQLFERMGDLPKAREAAHAALRLEPKFLPAKQQIGRILWAMGEPRQGERW
                     LAGVLRDTLSKRPATRYSSSYSRFVLRGTTPNAIRRDLARCQMAQNKYDAALRTLLQN
                     KNDRSPGQAVLESDVLYQLGRDAEAKKRLELALKARPRDQFLLKRLELLQNKTSPRRR
                     "
     gene            4402..5376
                     /locus_tag="P5079_03995"
     CDS             4402..5376
                     /locus_tag="P5079_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29181.1"
                     /translation="MIAPKVSLVIPVLNEEASLPGLLDSLDRALAGIPFEAIFVDDGS
                     TDGSLRLLEDRARADARVKVISFIRNFGQTPALAAGIDMARGDAIVCLDADGQNDPAD
                     IPGLLDELGRGADVVSGWRYPRRDPFLTRRLPSFLANHLISCVTGVRLHDYGCTLKAY
                     RRHVLQGLRLYGEMHRFLPAWCAWKGAKITEVRVRHHPRRLGKSKYGIVRTFKVLLDL
                     ITAKFFASYLTKPSYFFGGAGLALLFFSVIAGLLPIYDKFVLDSYGHLRIPFMILSIF
                     LGLLGAQFIVFGLLAEILIRIYYENKGERPYQVAKIVGVADDDPTRGK"
     gene            5380..6108
                     /locus_tag="P5079_04000"
     CDS             5380..6108
                     /locus_tag="P5079_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29182.1"
                     /translation="MIHLLLAAYNEGKALGNVLEKAEQSLSGQPHRVWVVDDGSSDNT
                     ANVAREWAKRIPLTLLRHPQNMGLGKALQTGLSQILPALAATDVLGIMDADNTQPPEL
                     LPVLVQRIQNNEADVVIASRFIAGGKSMGVPLFRRFTSAAARVLFRLFFHIPNVQDYT
                     CGYRVYRGELLLRAKNKWGVLLTEDGFAATVELLVKLSAFSPRVAEVPLLLRYDRKPT
                     PSKMPVFRTILKSLLLLRRLKKAK"
     gene            6105..7070
                     /locus_tag="P5079_04005"
     CDS             6105..7070
                     /locus_tag="P5079_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRY29183.1"
                     /translation="MTALRWLLALGISVAGLWFALHGADWETMSATVKNLRSPFWLAF
                     FPLVVLFEFTVRSIRWKLLLDPLKPAPLKVLFPITNAAFFINNVLPFRAGEFARIYWT
                     SQKTGVPLSSCVAVLAVDRLFDMATLLTMTFFVLLNKSALFQSRKPIVLFATVTLGLL
                     AAMALLARFPAPFKSWMEKPAVPRFLTRWAHQFIEGSQALRSTSTFLLAYLLSLSFWT
                     VFILVLRFLASLFSFSLSFTDAAFIEIAMCFGVLLPSAPGYVGTLEAAGVAAFAMLGF
                     DRNVALPFLLIVHFSQIMSTVVLGIPSLWIAGLKVHVPAADRPAP"
     gene            7067..8611
                     /locus_tag="P5079_04010"
     CDS             7067..8611
                     /locus_tag="P5079_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRY29184.1"
                     /translation="MMPRRLFSSPLAILTLLFSSALALRLWGITWGMPLKFAHVDESI
                     VTFYSLRVVAGDPNPRVFFDYPSLFLYLLAAVFQIVLSLGVLWGKIASVPQAMTAYLE
                     GDSVVFTLSARLLNVFLGMLTVLLVYHMGRLWAEEFGFDRRETPPDPGKPLFIGAAAA
                     AFLAVNRLHVLQAHYGTVDVPAALFSLLALHQTLLFWKTNSLKTGLWAAGMIGLAASV
                     KYYPAVFAGLLLPAPFVLKMRRPWKYAVFFLGAAAAGFFIGCPYALLTPHEFSARFFH
                     LFPKIVWSAQTGGAPLFLPTLGHILKNTGSPLLLLALYGLTGTLRRKNAVARVLAAAA
                     GVLVLFFGTWATQLEHYAMAAYPLLFLFAAQGLWELKSLHRAAPAVAAGLLVAWTALP
                     TFRQLQVLSQPDTRLTAYRWISQNIPPGSRLLRFAHTPEFRHTDPYSVQVDWENKRLL
                     ETLEPSEFVKSFDFVIYSSYRPGDDDLLPRIKHNFPLVGEFHGPAPRFPHHPAIYVFQ
                     TKKDIS"
     gene            8608..9363
                     /locus_tag="P5079_04015"
     CDS             8608..9363
                     /locus_tag="P5079_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29185.1"
                     /translation="MTSPKKKVIAVLPAYNAEKTVEKTLRDIPKDVVDEIILVDDASR
                     DRTAEVAQRLGLTVLKHDRNRGYGANQKTCYHEALKRRADVVVMIHPDYQYDARMTGL
                     LVHLIELDVCDLMFGCRIRTRKEALAGGMPLYKYLANRALTIFENLLMGTNLGETHSG
                     FRAYSRRLLETVPWEKNSDDFVFDQQMIIQARHFEFRMGDVPVPTRYEPESSSINFRR
                     SVVYGLGILWTLFRWKLHCWKLLRCSLFVDKSS"
     gene            9303..11363
                     /locus_tag="P5079_04020"
     CDS             9303..11363
                     /locus_tag="P5079_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29186.1"
                     /translation="METPLLETPPMFALRRQVLLTVYAAAAACLAAFYFIHYPIPGFF
                     LRLREIVTASVFLVLAAATGQRLGRLLRLPPTVAEDEAGPAPSATDNVLWGVGLGVPA
                     IALCLLFLGLAGAFRPAGFALLTVGLAAFSWKEILRLLSALRPLRVVSAETSGFHLFL
                     SVLGAAVLATALLCAFAPPTYYDSLVYHLALPAKYLQEGRIGFVPYNHYSHFPQGMEM
                     VYAWFLALGDDVSAQVFNVFIALLLAAAVWRLGKTRLSIDRPRWDIVLFLTAPCVTLL
                     ASETYVELPLAFWTLLCLWAAANGLERNDRRSFVLAGLMAGFATGLKYTGILTPAVLF
                     ALILFWPRPRTARERAGDALALFLPAFLVFLPWLVKNFLFTGGNPVFPFLPQWFPADN
                     VYMHRDAAGAYFRVLDEYRGTSPLLMELFGLPLRLVTNVVSFGGGYDVTGDLGWALPL
                     ILLPLAFLALPGHRVRGFFLVYLLAHALLWASLRPVLRFLFPVFPILCLLSGLGLVHL
                     FSQIPVWAGRALTAVLLLFFLSNGVLFYLVERVRDPFPAALGLMDREDYLRRKLDAYP
                     AWEFINKTLPADSRVLFVGDQRGYYCRRPYLAPMALLPQPLREWADTAETGEQLRSNL
                     LKLGFTHVFFNRKEADRLKGYRVLDLTEHGRTTWSDMRNRLSKDYDTPDATIYSLNAG
                     ARND"
     gene            11356..12381
                     /locus_tag="P5079_04025"
     CDS             11356..12381
                     /locus_tag="P5079_04025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRY29187.1"
                     /translation="MIESLYVGVFLIAFSVVIVLTPLMRKIALTLNIMDHPSSPVKTH
                     KHPTPYLGGMAIFLGIVTALLTARFFTHFPTGTLRSLRGILTGATFVVAVGLLDDLKP
                     GGLRFQWKFLFQFVAAFLLLLFDVRIKFIQPVWLADLLTVVWVVGVTNAFNIIDIKDG
                     LAASQAFMASLGFLFISLPTEEIYVNFAAAAAAGAAVAFIPYNMSGRMKIFMGDTGSL
                     MLGFCMAALSMGTSYTHVNEIGVFAPLLILGLPLYDTLFVSFLRIKQGKSPFLGSKDH
                     LALKLEALGLTSRQLVAALATVAALFSTAAYCVTWWPLHISLFVFAVAIAIGVYVVMK
                     LQKVVVP"
     gene            12288..13736
                     /locus_tag="P5079_04030"
     CDS             12288..13736
                     /locus_tag="P5079_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY29188.1"
                     /translation="MAAPHLPVRVRRGHRHRRLRRHEAPKGGRALKKNVPTTASPAPV
                     VIVGAGLAGLSCAYHLKNPFILCESEQTPGGTARSYTVDGFTFDHTGHLLHLHNEYTR
                     KLIRRLLPGNLRECARNAWIFSNGVYTRYPFQANLYGLPSKIVDECYLGLWEARRKYG
                     DDPLRTNHRLTFAEWSERLFGKGISEHFMFPYNRKLWTVPPDTLTPDWCGQFVPQPRL
                     QDVLVGAVTDRSTAFGYNVSFLYPHRGGIQVLSEAFAQKIGNVRLGCSLEKLLWQEKK
                     AVLSSGEMLPYAHLVSTIPLPELLKRLSPFPRELGEAAGQLRWTSVLCVNLGVNRRNI
                     SDKSWIYFPEDKFPFYRVGFPMNFSPQTVPDGCSSMYVEISSPPEKMPASPNSRNALL
                     RRIREGLIACNVLKRSDTFPVVSYLPIPYAYVIYDQRRRSALETIQPWLRARANTHSI
                     GRYGAWKYSFMEEAILDGKELAETLQKSSAAV"
     gene            13745..>14990
                     /locus_tag="P5079_04035"
     CDS             13745..>14990
                     /locus_tag="P5079_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY29189.1"
                     /translation="MDLLEKNPPPDDVVPAPPNLRQALFSVPVCFLFMFHPLFRGCWD
                     LWAQALVTFAWAVLAIFAGLFLIRKTEREDSLHEIQIPLTLFLLVSFLSSSFSRFPHS
                     AVPAFLADGTALAFFVFFLISTPLWRARYLRALAGGGTVAAFLALFQLHLHPHAPPTG
                     PFVNPNLLAAYLLLTLPLTGALLLDETSRSSRLIWGGALLAQTAVLFSTASFMAFLAL
                     LAEGLCLALWPASPVSAKRRKILLALTAGAAVGGLVFFRHDWARLIHWEADRTAWWRT
                     AFQMWLNNPLWGVGPGAFGEAYPGYRADPLGLNSLYAHNVFLEWLAERGGAGAGAALF
                     LIFLLWIGRRRNAAENKFTVPVLTAMGGFLFFNAAHFGLSFPSLYWVFWSLAGYLWAT
                     AAPPPDRGGRTEKSRRALRTGLL"
BASE COUNT         2466 a         5259 c         4128 g         3137 t
ORIGIN      
        1 cggctggtca tcgaattgaa ggaggcggtg gcccagcctt atgaggccgc ccccgaagat
       61 gccgtgttga aacgcatccg ctgcgctcaa ttccagacct ccccgaagaa gatcgcccgc
      121 gtcgtcttgg acctttccca gcacacctcc tacgaaacgg tccgggaggg gaacgacctc
      181 ctcctgcggt tcaccggaac cacggcggcc ggcgccgggg ccgccccaac ggctcaaaaa
      241 aataccgcgg ccgccgaccg cagcaccgct tctcccgcca tggaatcggt cccgcctcac
      301 aaatcgcctt tgcccgaaaa gaccgtttcc ggcgaaacgc aggcctccgt cggggccgcc
      361 gcggcgacgc ccggcgcctc caaaaccccg gcgaaaaatg cgaaggacct gctcgcctcg
      421 ctccccaaac atccggtcac catcgacttc gaggaggccg acatccggga cgtcatccgc
      481 gtcctctcgg agatgtgcgg catcaacatg atccaaagcc ccgacctgcg cggcatggtc
      541 accatccacc tcgaccaggt ccccttcaac gaggcgttta acaccatact gacgatgtac
      601 ggcctggtgg cccaacaaat gggggagaac atcctccgca tcctcacccc ggaagccctc
      661 agcgtggatc gttcccgcgc ggtcaccacc tacaagacct ttacgttgaa ctacgccaac
      721 gcctcggaca tccttcagca cctcgcctcg gtccgcatct ccccgatggg gcgcgccacc
      781 gtggacgaac gcaccaactc cctcatcgtc acggacacgc ccgaagggat cgccgccgcc
      841 gaacgcctga tcgccgaact ggacgaaaaa ccgcaacagg tcatgatcga ggcgcgcatg
      901 gtggaggtca ccctcaccaa cagtctggac ctcggcatcc aatgggaata cgccaaagag
      961 agcaagacgg ccaccaacca gacgatcatc gggcagcgga acgtcgaagc cggcgggacg
     1021 acccccacgg aaggcaaggc cagcctcgtc acttcggccg gggtcgaatc cgcctttgcc
     1081 tccggttccc gcggcacggg cgtctccctc cccggtccgc aatcgaccgc catcacgttc
     1141 gggttcatca acaacaccaa cctcctcacc gccacgctga acgcgctggc cacctcggga
     1201 cagacgaaaa tcctctcctc gccgaaggtc atcaccgtca acaacaaaca ggcgaagatc
     1261 caggtgggct cccagatccc cttctccatc accaccgtgt cgggaacggg catcgccacc
     1321 cagtcctttc aaatggtgga cgtcggcatc atcctgctgg tcacccccac catcaacgcg
     1381 gacaaccgca tccgcctcaa ggtgcagccc accgtcagca ctcccggcaa cgtgacggcg
     1441 gccggccccg aaatcagcac gcgcaacgcg gacacggagg tcatcatcca ggacggcgag
     1501 acgctggtca tcgccggact gatcgacgaa cagatgcggg agagcgcctc caaggtgccg
     1561 ctgctgggcg acattcccgt cctcggcgtt ttcttccgga aatcctccga ttccaaacag
     1621 cgcacggaac tcctgatctt cgtcacacca aaaatcatcc gggactgatc gcaccgacca
     1681 cgtgacgtga acgcccccgc cgacggcgcc aaggattctc catccttgac cgcccttctt
     1741 cgggaagccg agaattaccg gcaacagaaa atatgggaag ccgccattcc gtcctatcaa
     1801 aaagcgctca agctggaacc cggccatgtc cgctctcatc tggggctggg gcacgtgtat
     1861 gccgccaaag gacaggaggc ggggtcgagg ggattcctcc tgctctccgc ggaacatttt
     1921 cgaaaagcgc tcgccacgga cccgaactgc cgggaagcgc acgagtccct cctggcggtc
     1981 accgccaaga tggggcagtg ggacgagttg ctccaggact acaaggcgcg cctggtgaaa
     2041 gatccggaga acccttcgct gaaggacgcg atgaaaaaga tccaggccgc tttcctcctg
     2101 cggacgaccc cgtcggccgc gccgccgaag ccgcaggggt tgttatggac ttttttcaac
     2161 gtggggctcc ccctgctggg cttcgtctct ttttttctgg cgctgatttt cagaatacag
     2221 aacatcatcc cgtcctggac gaccctgtat gtcggatttt tgcgcatggg aaccttcttt
     2281 ttcgtcgtct attttctcct gcgcctcttc ctcaaatgaa agcggacccg catgactgaa
     2341 gaaaccctca aagaaccccg aaaagacggc gccgtggccg ccctgtcgct cgtgctgttc
     2401 gcggcggcgt tttttgctca cggggggaaa atcgttgcgc tttggagccc cgtctccgcc
     2461 ggacttctct tgacggcctt cctcctgctt cgccgccggc cggtcttcct cctcccgtcc
     2521 ttccttcccg gcgtgctggg ttttctgggc tggggatttc tggccacctt gtcctcgccg
     2581 agccccttga ccagcctctg gacgatgtcc aaaatcggct ccgtggcggc cttcggcgtc
     2641 gtggcctggt tctgctgggg ggagaaggcg aacaaaatat ttcgcggtct ggtgatggcc
     2701 gccgcgctcg cccagtccct gctcgttttt tcaagccctt tgttcggccg tccgatattg
     2761 ctcctgcccg gcaaccccca ctatatgggc ctctggatga ccgtcgggtg tctcctcggc
     2821 ctcaacgcgc tcgtcgcccc ggacatctcg aaacgactcc gctggacctt cgccggcatc
     2881 accgtttttc tggccgcggc gacgttctgg ctcccttcgc gggccgggac gatcgccctg
     2941 gtcgtggggg cgtctttttt cctgggaaga cgtttcggtc gcaaggccct cctgtcctgg
     3001 ctcctttttg ttcttctgct cgcctggctg ttcgtcggct ccctggaacg actgctgacc
     3061 cgctcggggg atcccttggc ttggaaacgg ctggacatct ggcgggcggc ggtgaccggg
     3121 ctttcgattc atccttacgt cggctggggt ccgggacagt ttgaaacgct ctatcaaggg
     3181 tgccgccccc cgcagacgga cctgccggtc tggtacggcg taacgacggc cttcgcgcac
     3241 aacgactatc tccaactcgc cacggaatgc ggactcccgg ccgcggtctt cctcctttgg
     3301 ggacttgcct gtttcttttt ctacaaccgt cctcgcgacg gccccgccct ggcggcccac
     3361 gcggccgttc tcgccatcgg cgtcttctgc ctgttcaact tccccatgtt ccttcccgtc
     3421 aacggcctgt tggccgccgg cctcctggtc cagggggaaa cccggaccga aaaattccgg
     3481 ctcgcgctct ctccccggct cggttcgttc ttccgtcgga cgatcctcac cctgacggct
     3541 ctctttgcgg tctccgccgg cgcggttttt ctgatcgaac tccccgcgac ggccggagtt
     3601 cgttcactct cccgctaccg cctttggaac ccggcgtctc tcctggaaca ggcggacgct
     3661 ttcctgcact cggccgataa gaaagatgtc catccggaat cggccgaacg tctcctgcgc
     3721 cggatcgttc tcttcgacag gagcaacgcc aaggcgtggc gcgacctgtc gcatcttctc
     3781 ttgatgcacc gcgcggaccc ggacctggaa accgcccggc gggctctcgc caaagcgctc
     3841 ttttggcatc ccaccgaagc gacctggtat ctggaattga gccagctctt cgagcggatg
     3901 ggggacctcc ccaaggcccg ggaagccgcc cacgccgccc tccggctgga accgaaattt
     3961 ctgccggcca aacaacagat cggccggatc ctttgggcga tgggagaacc tcgacagggg
     4021 gaacgatggc tcgccggcgt actccgggac accctctcca aacggccggc gacccgctat
     4081 tcctcgtcgt attcccgttt cgttctgcgc ggcaccacgc cgaacgccat ccgccgggac
     4141 ctggcccgtt gtcaaatggc gcagaataaa tacgacgccg ccctgcggac gctgcttcaa
     4201 aacaagaacg accggagccc cggacaggcc gtgttggaat cggacgttct ctatcaactg
     4261 ggccgagacg cggaggcgaa aaaacgtctc gaactcgccc tcaaggcccg gccgcgggat
     4321 caattcctcc tgaaacgcct ggaactcttg cagaacaaaa cgtctccgag acgccgttaa
     4381 gcccggccca aaggaaccgc catgatcgct cccaaagtca gcctggtcat ccccgtcctc
     4441 aacgaagagg caagccttcc gggactgctg gattccctgg accgggccct cgccggaatc
     4501 cctttcgagg ccatcttcgt ggacgacggt tccaccgacg gaagccttcg tcttctcgag
     4561 gacagggccc gggccgatgc ccgcgtcaaa gtgatcagtt tcatccggaa cttcggccag
     4621 acgccggcgc tggcggccgg cattgacatg gcccgcggag acgccatcgt ctgtctggat
     4681 gccgacggcc aaaacgatcc ggccgatatc cccggcctgc tcgacgagtt gggccggggg
     4741 gccgacgtgg tcagcggctg gcggtatccc cgtcgggacc cttttctcac gcgccggttg
     4801 ccctcgttcc tggcgaacca cttgatctct tgtgtgaccg gcgtccgttt gcacgactac
     4861 ggctgcaccc tcaaagccta tcgacggcat gtcctccaag ggctgcgcct ctacggggag
     4921 atgcaccggt tcctgccggc ctggtgcgct tggaaaggcg ccaagatcac cgaggtccgg
     4981 gtgcggcacc atccgcgccg tctgggaaaa tccaaatacg gcatcgtgcg gacgttcaaa
     5041 gtccttctcg atctgatcac cgcaaaattc ttcgccagtt acctgaccaa acccagttat
     5101 ttcttcggcg gggccgggct ggccctcctc ttcttcagcg tcatcgccgg cctcctcccc
     5161 atctacgaca agttcgtcct ggacagttac ggccatctgc gcatcccttt catgatcctt
     5221 tcgatcttcc tcggtctttt gggcgcgcag ttcatcgtct tcggcctgct ggcggaaatc
     5281 ctcatccgga tttattacga aaacaagggc gaacgtccct atcaggtcgc gaaaatagtc
     5341 ggcgttgccg acgacgatcc cacccgcgga aaataaaccg tgatccatct gctcctggcc
     5401 gcctacaacg agggcaaagc cctcgggaat gttttggaaa aagcggaaca gtccctctcc
     5461 ggccaaccgc accgcgtctg ggtcgtggat gacggctcga gcgacaacac cgccaacgtg
     5521 gcccgggaat gggccaaacg catccccctg acgctcctgc gacatcctca aaacatgggg
     5581 ctggggaaag ccctccagac cggcctctcc caaatccttc cggccctcgc cgccaccgac
     5641 gtcctcggca tcatggacgc cgacaacacc cagccgcccg aactcctgcc ggtcctcgtt
     5701 caacgcatcc agaacaacga agccgacgtg gtcatcgcct cccgcttcat tgcggggggg
     5761 aaatccatgg gggtccccct gttccgccgg ttcaccagcg cggccgcccg cgtcctgttc
     5821 cgcctttttt tccacatccc gaacgtgcag gattacacct gcggttaccg cgtctaccgg
     5881 ggggaactcc ttttgcgggc gaaaaacaag tggggcgttc tcctgaccga agacggcttc
     5941 gccgccaccg tggaactcct cgtgaaattg tcggcctttt ctccgcgcgt ggcggaggtg
     6001 ccgctgctcc tccgctacga ccggaaaccg acccccagca agatgccggt gttccggacg
     6061 atcctgaaat ccctcctcct cctccggcga ctcaagaagg cgaaatgaca gcactccgtt
     6121 ggctgttggc cctcggcatc agcgtcgccg gcctctggtt cgccctgcac ggggccgact
     6181 gggagacgat gtcggccacc gtgaagaacc tgcgttctcc gttttggctg gcgttttttc
     6241 cgctggtcgt cctgttcgaa ttcaccgtgc gttccatccg ctggaaactt ctcctcgatc
     6301 ccctcaaacc ggcgccgctg aaagtcctct tccccatcac caacgccgcg ttcttcatca
     6361 acaacgtcct ccctttccgc gccggagagt tcgcccgcat ttactggacc tcccaaaaga
     6421 ccggcgtccc cctcagcagc tgcgtggcgg tgttggcggt ggaccgcctt ttcgacatgg
     6481 cgaccctcct gacgatgacc ttcttcgttc tcctcaacaa atcagccctc ttccaatccc
     6541 gaaaacccat cgtccttttc gccactgtga cgctcggcct cctggccgcc atggccctgt
     6601 tggcgcgttt cccggcgccc ttcaaatcct ggatggaaaa accggccgtc ccccgtttcc
     6661 ttacgcgatg ggcccaccaa ttcatcgaag gatcgcaggc cctgcggagc acctcgactt
     6721 ttcttctcgc ctatctcctc tccctttctt tctggaccgt tttcatcctg gtgcttcggt
     6781 tcctggcctc cctgttctcc ttctccctgt ccttcacgga cgccgccttc atcgagatcg
     6841 ccatgtgttt cggcgtcctt cttccgtcgg cgccggggta tgtggggacc ctggaagccg
     6901 ccggggtggc ggccttcgcg atgctcggtt ttgaccgcaa cgtggccctg ccttttctgc
     6961 tgattgtcca tttctcacag atcatgagca ccgtcgttct gggcatcccg agtctttgga
     7021 tcgccgggtt gaaagtgcac gtcccggcgg ccgatcgtcc tgccccgtga tgccccgccg
     7081 tcttttttcc tctcccctgg cgatactcac ccttcttttc tcctccgcgc ttgcactgcg
     7141 gctgtgggga atcacctggg ggatgccgct gaaattcgcg cacgtggacg aatccatcgt
     7201 caccttctat tccctgcgcg tggtggccgg agacccgaac ccccgggtct tcttcgatta
     7261 cccctcgttg ttcctttacc tcctcgccgc cgtctttcag atcgttctct ccctgggcgt
     7321 gttgtggggg aaaatagcct ccgtgcccca ggccatgacg gcctacctgg agggggattc
     7381 cgtcgttttc acgctttcgg cccgcctgct gaatgtcttc ctcggcatgc tcaccgtcct
     7441 tctcgtctat catatggggc ggctctgggc ggaggagttc ggtttcgacc gccgggaaac
     7501 gcctccggac cccgggaaac ccctgttcat cggcgcggcc gcggcggcgt ttttggccgt
     7561 gaaccgcctt cacgtgctcc aggcgcatta cggcacggtg gatgtgccgg ccgccctttt
     7621 ttctctcttg gcccttcacc agacactcct gttttggaaa acgaactcgc tcaaaacagg
     7681 cctctgggcg gcggggatga tcgggctggc cgcgtccgtc aaatattatc cggcggtgtt
     7741 cgccgggctt ctcctgcccg cgccgttcgt cctgaaaatg cgaaggccct ggaaatacgc
     7801 cgtcttcttc ctgggcgcgg ccgccgccgg ctttttcatc ggttgcccct acgccctcct
     7861 caccccccac gaattctcgg cgcgcttctt tcatcttttt ccaaagatcg tctggtccgc
     7921 ccaaaccggc ggggcgccgc tgttcctgcc cacgctcggg cacattctaa aaaacaccgg
     7981 ctctccgctc ctgctgttgg ctctgtacgg cctcaccggc acgctccgac ggaaaaacgc
     8041 cgtcgcccgg gtcctggcgg ccgcggcggg ggtcctggtc ctcttcttcg gcacctgggc
     8101 cacccagctg gaacattacg ccatggcggc ctacccgctc ctgttcctct tcgccgcgca
     8161 gggactttgg gagttgaaat ccctccatcg ggcggcgccg gccgtcgccg ccggcctgct
     8221 ggtggcgtgg accgccctgc cgacgttccg ccagcttcag gtcctgtccc agcccgacac
     8281 ccgcctcacc gcctaccgat ggatatctca aaacattcca cccggaagcc ggctgctccg
     8341 tttcgcccat acgccggagt tccggcacac ggacccctat tcggtccagg tggactggga
     8401 aaataaacgc cttctcgaga cactggagcc gtcggaattt gtaaaatctt tcgactttgt
     8461 catctacagt tcctatcgcc cgggagacga cgacttgttg ccccgcatca aacataattt
     8521 tcctctggtg ggggaatttc acggtccggc gccccgtttt ccgcaccacc cggcgatcta
     8581 cgtttttcaa acgaagaagg atatttcatg acgtcaccga agaagaaagt gattgccgtc
     8641 ctgcccgcct acaacgcgga aaagaccgtc gaaaagacgc tgcgggacat cccgaaagac
     8701 gtggtggacg agatcatcct ggtggacgac gcctcgcgcg accgcaccgc cgaggtcgcc
     8761 caacggctcg ggctcacggt cctcaaacac gaccgcaacc ggggatacgg ggccaatcag
     8821 aaaacctgct atcacgaggc gctgaaacgc cgggccgacg tcgtggtcat gatccatccc
     8881 gattatcagt acgacgcccg catgaccggc ctcctcgtcc atctcatcga actggacgtg
     8941 tgcgacctga tgttcggctg ccgcatccgc acgcgcaaag aggccctggc cggcgggatg
     9001 ccgctgtaca aatacctcgc caaccgggcc ctgaccatct tcgaaaacct cctgatgggc
     9061 accaacctgg gagaaaccca cagcgggttc cgcgcctatt cccggcgact gttggaaacc
     9121 gtcccctggg aaaaaaattc ggatgatttc gtcttcgacc agcagatgat catccaggcc
     9181 cggcatttcg aattccgcat gggcgacgtg cccgtcccca cccgttatga gcccgaatcc
     9241 tcctccatca atttccgccg cagcgtggtc tacgggctcg gcatcctctg gacgctcttt
     9301 cgatggaaac tccactgttg gaaactcctc cgatgttcgc tcttcgtcga caagtcctcc
     9361 tgaccgttta cgccgcggcc gccgcctgcc tggccgcctt ttattttatc cattacccga
     9421 tccccggttt tttcctgcgt ctgcgggaga tcgtcacggc atcggttttc ctcgtcctcg
     9481 ccgccgccac cggtcagcgg ttgggccgcc tgctgcggct gccgccgacg gtcgccgagg
     9541 acgaagcggg accggccccc tccgccacgg acaacgtcct ctggggcgtt gggctcggcg
     9601 tccccgccat cgccttgtgt cttctgttcc tgggattggc cggcgccttt cggccggcgg
     9661 ggttcgccct cctgacggtc ggcctggcgg ccttttcctg gaaagaaatc ctccgtctcc
     9721 tctcggctct ccggcccctt cgcgtcgtgt cggccgaaac ttccggattc cacctgttcc
     9781 tgtccgtcct gggcgcggcc gttctggcga ccgccctgct ctgcgccttc gccccaccga
     9841 cttattacga cagtctggtc taccacctgg ccctgcccgc caaatatctc caggagggac
     9901 gcatcggctt cgtgccgtac aaccactaca gccatttccc gcaggggatg gagatggtgt
     9961 atgcctggtt cctggctctg ggagacgacg tctccgccca agttttcaac gttttcatcg
    10021 ccctgctcct ggcggccgcg gtgtggagac tcgggaagac gcggctgagc atcgaccgtc
    10081 cgcgatggga catcgtcctc ttcctcaccg ccccgtgcgt gacgctcctg gcctccgaaa
    10141 cctatgtgga actgccgctg gctttctgga cactgctctg cctgtgggcc gccgcgaacg
    10201 gccttgaaag aaacgatcgc cgatctttcg tgttggccgg actcatggcc ggcttcgcca
    10261 ccggcctgaa atacaccggc atcctcacgc cggcggttct cttcgcgctg atcctgttct
    10321 ggccccggcc ccggaccgcg cgagaacgcg ccggggacgc cctcgccctc ttcctgcccg
    10381 ccttcctcgt tttccttccg tggcttgtga aaaacttcct tttcacgggc ggcaaccccg
    10441 tttttccatt cctcccccaa tggttcccgg ccgacaacgt gtacatgcac cgggacgccg
    10501 ccggcgccta tttccgcgtg ctcgacgaat accgcggcac cagcccgctg ctcatggaac
    10561 ttttcgggtt gccgttgcgt ctggtgacca acgtggtttc gttcgggggg ggatacgacg
    10621 tcaccggaga cctcggctgg gccctgccgt tgatactcct ccctctggcg ttcctggccc
    10681 tcccgggaca tcgcgttcgc ggtttcttcc tcgtctatct tctggcgcac gccctcctgt
    10741 gggcctctct gcgtccggtc ctgcggttcc tcttccccgt ttttcccata ctctgtctgt
    10801 tgtccggcct gggccttgtg catttgttct cccaaatccc cgtttgggcg ggacgcgcgc
    10861 tgacggcggt cctgcttctc ttcttcctct ccaacggcgt cctcttctat ctggtcgaac
    10921 gggtccgcga cccgttcccg gcggcgctgg gactgatgga ccgggaggac tatctccgac
    10981 ggaaactgga cgcctacccc gcctgggagt tcatcaacaa aacgcttcca gccgacagcc
    11041 gggtcctctt cgtgggggac caacgcggct attactgcag gcgcccctac ctggcgccca
    11101 tggccctgct gccccagccg ctccgggaat gggccgacac cgccgagacg ggcgaacaac
    11161 tccggtcgaa tctgttaaaa ttgggattca cgcatgtttt tttcaaccgg aaagaagccg
    11221 accgcttgaa agggtaccgg gttttggacc tgaccgaaca cggcagaaca acgtggtcgg
    11281 atatgcggaa ccggctgtcg aaagactacg acacgccgga cgcgacaatc tattcgctga
    11341 acgcgggagc acgcaatgat tgaaagcctc tacgtgggtg tttttctcat cgccttttcg
    11401 gtcgtcatcg ttctgacacc gttgatgcgg aagatcgccc tgacgctgaa catcatggac
    11461 cacccgtcct ccccggtcaa aacgcacaaa caccccaccc cctatctggg ggggatggcc
    11521 atcttcctgg ggatcgtgac ggccctgctg accgcgcgtt tcttcacgca ttttccgacc
    11581 ggcacgctgc gctccctgcg ggggatcctc acgggggcga cgttcgtggt ggccgtggga
    11641 ctgttggacg acctcaaacc cggcgggctc cgtttccaat ggaaattcct tttccaattc
    11701 gtcgcggcct tcctgttgct tctgttcgac gtccggataa aattcatcca acccgtgtgg
    11761 ctggcggatc tgctcacggt ggtctgggtg gtgggggtca ccaacgcctt caacatcatc
    11821 gacatcaaag acggcctcgc cgcctcccag gccttcatgg ccagcctggg gttcctcttc
    11881 atctccctcc cgacggagga gatctacgtc aacttcgcgg cggcggccgc cgccggcgcg
    11941 gcggtggcgt tcatccctta caacatgtcg gggcggatga agatcttcat gggggacacc
    12001 ggcagtctca tgctcggctt ctgcatggcg gcgctttcga tgggcacgtc ctacacccac
    12061 gtcaacgaga tcggcgtgtt cgcccctctt ttgatcttgg ggctgcccct ctacgacacc
    12121 ctcttcgtct ccttcctccg catcaaacaa gggaaatcgc cgttcctggg atcgaaagac
    12181 cacctggctc tgaaactgga ggcgctgggc cttacttccc gacagttggt cgccgcgctc
    12241 gcgacggtgg cggcgctctt ctccaccgcc gcttactgcg tcacgtggtg gccgctccac
    12301 atctccctgt tcgtgttcgc cgtggccatc gccatcggcg tctacgtcgt catgaagctc
    12361 caaaaggtgg tcgtgccctg aaaaaaaacg tcccgacgac ggcctccccg gcgcccgtcg
    12421 tcatcgtcgg cgccggcctg gccggcctct cctgcgccta tcacctgaaa aatcctttta
    12481 tcctctgtga aagcgaacaa accccggggg gaaccgcccg gtcctatacg gtggacggct
    12541 tcacgttcga ccacaccgga catctcctgc atctgcacaa cgagtacacc cgcaaactca
    12601 tccgccgcct gctgcccggc aacctccggg aatgcgcccg caacgcctgg attttttcga
    12661 acggcgtcta tacccgctat ccgtttcagg ccaacctcta cggcctgccg tccaagatcg
    12721 tggacgaatg ctatctcggt ctctgggaag cgcggcggaa atacggcgac gacccgttga
    12781 gaaccaacca tcgcctcacg ttcgccgaat ggagcgagcg gctcttcggc aagggcatat
    12841 cggaacattt catgttcccc tacaaccgga aactctggac cgtgcccccc gacacgctga
    12901 cgcccgattg gtgcgggcag ttcgtcccgc aaccccgcct gcaggacgtc ctcgtcggcg
    12961 ccgtgacgga ccggtccacg gccttcggct acaacgtctc cttcctctat ccccaccgcg
    13021 gcggcattca agtgctctcc gaagccttcg ctcaaaagat cgggaacgtc cgcctgggct
    13081 gctcccttga aaaactgctc tggcaggaaa agaaagcggt gctctcctcg ggcgagatgc
    13141 tcccttacgc gcacctcgtc tccaccatcc cgctgcccga acttttgaaa cgcctcagcc
    13201 ctttcccgcg ggagctgggc gaggcggccg gccaactccg ctggacatcc gttctctgcg
    13261 tcaacctggg cgtgaaccgc cgcaacatct ccgacaagtc ctggatctac ttccccgaag
    13321 acaagttccc gttctatcgc gtggggtttc cgatgaactt ctctccccag accgttcccg
    13381 acggctgttc ctcgatgtac gtcgagatct cctccccgcc ggaaaaaatg cccgcctccc
    13441 cgaactcccg gaacgccctc ctccgccgga tccgggaggg gctgatcgcc tgcaacgtgt
    13501 tgaagcgctc ggacactttt ccggtggtga gctatctgcc catcccgtac gcgtacgtga
    13561 tttacgacca gcgccgccgg tcggcgctcg aaaccatcca accgtggctg agggcccggg
    13621 ccaacaccca ttccatcggc cggtatggcg cgtggaaata ttccttcatg gaagaggcca
    13681 ttctggacgg aaaggaactg gcggaaacgc tccaaaaatc ttccgccgcc gtctgacatc
    13741 cccgatggac ctgctcgaaa aaaatcctcc gcccgacgac gtggttccgg ccccgccgaa
    13801 tctccggcag gccctctttt ccgtcccggt ctgttttctt tttatgttcc acccgctttt
    13861 ccgcggctgt tgggacctct gggcccaggc gctggtgact ttcgcctggg ccgttctggc
    13921 gatcttcgcc ggcctgttcc tcatccgaaa aaccgagagg gaagactccc ttcacgaaat
    13981 acagatcccc ctgacgcttt tcttgctggt ctcttttctt tcttcgtcct tcagccggtt
    14041 cccgcacagc gcggtgccgg cgtttctggc ggacgggacc gccctggcgt tcttcgtctt
    14101 tttcctgatc tcgacgcccc tttggcgggc ccgctatctg cgcgccctgg ccggcggggg
    14161 gacggtggcg gcgttcctgg cgctctttca gctccacctt cacccgcacg ctccgccgac
    14221 cggccctttc gtcaatccca acctcctggc cgcctatctg cttctcaccc tcccgctgac
    14281 cggcgccctc cttctggatg aaacctcccg ttcctcccgc ctgatctggg gcggggcgtt
    14341 gttggcccag accgcggtcc tcttttcgac ggcctcgttc atggcgtttt tggccctgct
    14401 ggcggagggc ctctgccttg cgctctggcc cgcaagcccg gtgtcggcga aacgccggaa
    14461 aatcctgctg gcgctcaccg cgggcgcggc ggtgggaggc cttgtcttct tccggcacga
    14521 ctgggcccgt ctcatccact gggaagccga ccgcaccgcg tggtggcgga ccgcttttca
    14581 aatgtggtta aataatccgc tgtggggcgt ggggccgggg gcgttcggcg aggcctatcc
    14641 cggttaccgt gcggacccgc tggggttgaa cagtctttac gcccacaatg ttttcttgga
    14701 atggctggcc gaacgcgggg gggccggggc gggggcggcg cttttcttga tctttttgtt
    14761 atggatcggc cggcggcgga acgcggcgga aaacaaattc accgtgccgg tgctgacggc
    14821 gatgggagga ttcctcttct tcaacgccgc ccatttcgga ctttcgttcc ccagtctgta
    14881 ttgggtcttc tggagtctgg ccgggtatct ctgggccacg gcggcgccgc ccccggaccg
    14941 cggcggtcgg acggaaaaat cgcgacgcgc cttgcgaacg gggctgttgg
//
LOCUS       DAOTFD010000040        14985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2742_length_14985_cov_4.137709, whole genome shotgun sequence.
ACCESSION   DAOTFD010000040 DAOTFD010000000
VERSION     DAOTFD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_14985_cov_4.137709"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5079_04040"
     CDS             complement(<1..524)
                     /locus_tag="P5079_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29190.1"
                     /translation="MIPLIKNIRGHLRAGHIAVLLVLLALLLPPGWLSAKSWEWGLRW
                     LYVLLLAFLASSLLTPLCIALAHRFDILDIPDARKTHGRPIPRLGGLAIFLSVLLTVG
                     RNFQFSRELTGLITAGTLIFVTGLIDDARGLSAKFRLAAQVAAALILVFFNVHITFVP
                     PSVPGEHLWEILITV"
     gene            complement(528..1145)
                     /locus_tag="P5079_04045"
     CDS             complement(528..1145)
                     /locus_tag="P5079_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY29191.1"
                     /translation="MNVLAIGPHPDDLEYGCGGTLAKLSDKGHRIHLLVITKGGMGGA
                     PAVREKEQEKSAKLLKARLHWGSWKDTEVPLEKALIDRVEAVIADTQPLLIFVPYHND
                     THQDHRNVSQATVTATRYVRNVLFYEVPSSVDFTPNLFVDIGKHIEKKFALLKAHHSQ
                     VYQTKVPGLSIIENARSAAIFRGFQNRVKYAEGFVPVRLALDFAL"
     gene            complement(1166..1972)
                     /locus_tag="P5079_04050"
     CDS             complement(1166..1972)
                     /locus_tag="P5079_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY29192.1"
                     /translation="MYEAYWGLTIKPFENTPDPRFLYNDPQHEEALSRLLYVVREGKG
                     AGMLTGVYGCGKTLLGRTLLRELEKDVYRVAYITNPRLDDVELLRMVAHGLGLPDLPT
                     RKADVLVALEKIFDENLRDGRRTVVVVDEAHAIEDVRLFEELRLLLNFQTEEKFLVTL
                     FLLGQPELQPKVDSNKQLAQRLAMRYQLDPLGAAHVRGYIEHRMQVAGSARPVFTPEA
                     VHVIFERSGGIPRRINHICDVSLLTGYSKNAAAVDRDIAREAIESLGGAG"
     gene            complement(1985..2671)
                     /locus_tag="P5079_04055"
     CDS             complement(1985..2671)
                     /locus_tag="P5079_04055"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRY29193.1"
                     /translation="MIMAAHQPQYLPWLGYFHKMASCDVYVYLDDVQYKKREFQNRNK
                     IRVKDGSQWLTVPVVTKNCYYQEIREVAIDPTSDWGKDHWTAVSLAYSKALFFKSHAG
                     ELETFYRKKWGGLAEMSEALIEFHRRLLEIDTPIVRSSEFHVRSKKTERLVALCLAVG
                     ADTYLSGQGARDYLDESLFARAGLKVIYQEFKHPEYPQAYPGFEPYMAALDLLLNCGP
                     ESRNILLPKN"
     gene            2806..2964
                     /locus_tag="P5079_04060"
     CDS             2806..2964
                     /locus_tag="P5079_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29194.1"
                     /translation="MENSKTFIIPVPGDSLTGGPEALHQLAREINDLPDCQAFIHYTG
                     RKDPRVAE"
     gene            2974..3909
                     /locus_tag="P5079_04065"
     CDS             2974..3909
                     /locus_tag="P5079_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29195.1"
                     /translation="MYATRETADPREDGAVLVLGELYDPSEWNFPEFKAKWVWWLSCR
                     RHYPLEAYRGWGHLFQSEYARLLFEKIGLSGFMLTDHIRPDFFESPPAPSSKKDLIIF
                     SGSRSALNVLRLKLTEPRLRLMPLRGLGAPQIVRRMDEAKIYLDMGLHTGRDRLPREA
                     ALRGCLVVMPRQGAAAGPADVPIPDEYRWPAGRVAGLGDFLRRLLKEYDERVRDFSGY
                     RDWIGNQRAAFQKEVRHFSEVCRDVRPPAVSPAEKILPQLDAQVLELEESLGRLKRES
                     ADLAVRVENVLMLMPLKVRLAALLNAAVGRLRRPR"
     gene            3906..4460
                     /locus_tag="P5079_04070"
     CDS             3906..4460
                     /locus_tag="P5079_04070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY29196.1"
                     /translation="MKSAAGPGVCVDLGCGKNKQPGCFGVDAVLLDGVDVVGDLSRGN
                     IPFRTSSVRGVYCSHFLEHLEDPGALLKEIHRVLVPGGRLFLRVPHYSNYAGFNPAHR
                     SFFGQNFFDIYDPDTDLGKRFDYFSDEKFRIVNKELRLFKTWKLPYTYLLQWLASWKP
                     AAYECHFAFLSPAEEIYVEMEVLK"
     gene            4461..6014
                     /locus_tag="P5079_04075"
     CDS             4461..6014
                     /locus_tag="P5079_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRY29197.1"
                     /translation="MGVPLSKTITRNTLYSAYLTALQFVVGLVMVPYLVFRLGLDYFG
                     IWSTVFAVAGYLGFIDLGASTSFVPYFAAAAARKDEQEFNRVFNTGLLFYGAAALMLT
                     LAAPFLAGELLDLLKIPPPLRGDTRFALTWAIWIYVYGLWAGVFRSVLVGFQRMDISN
                     RIEMGLALAASAGAVLVLELGFGLRGLILSRAVVVFGASLFTLRAALKMEPIRPRFSP
                     RLFSVDMLKKCFAYGVNTQISYLCSMGTLYLANIVIARYLGLAAVGVYEIGRKIAQTA
                     RSLPMVLLPALVPASADLYGRSDGDKLMRLYRTGSKYMSAVTVPLAAFLVFQMERIVR
                     VWVGPGYGPSVLIGQVLVAAYLFNMLTGVPGSIARGIDQVRLEARSSTVVLFLTVLFM
                     GAAVRSDAGLAGFAVAIWLAMSTGTLYFIVLAHRKIFHIPVGPFLAQMLLKPSASCLA
                     AGAVSAGLFWIFPPPGGRFAEGLALATVFSVFAAAFMLLLHFVRYFDAEETRIIFSAW
                     RQLKSSDRR"
     gene            6011..7507
                     /locus_tag="P5079_04080"
     CDS             6011..7507
                     /locus_tag="P5079_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29198.1"
                     /translation="MKARPIFSAGFSLLSGIFVFLCLAKLCMEPMMLGRDSAIYFLAA
                     RSLLSGHGYVLEIGGPALQPLYPPGYSLFLSLFVWTFPKHVWVLRLTSLLPCLGAALI
                     LWKWFGKSRRGLLFCGIFFFNPVTLDLALAVRPEMFFIFLVSLILWLARDDVFGGERG
                     YFGRLGGVLLLACLSVYVKTAGIALVLSAGLCVWLSRWKISHKLLATAFMAALLSPCF
                     FAQSKDYVGRYFFQSDIVADKVAFGWRPFIERISGNVAIHLGRNMADFVFGGVFQNIT
                     RAASGSFFFGAKTALSLFVGGLVLAGWWRSLRRRTGLAGLFFIFYTGLVLSWPWYKME
                     YRFLFPLFPLLFYYFWAGARGLMPRAVFGKAILVCFMGAAVFFSLFQAVWFVGVRKMR
                     APSVWSDVVWAQRTREFLDGMEWIRTHAEKDAALLCGDPYAVHLYTGRKCFLFPRAVS
                     VSELPGEAGYVYATAGRKDEMDFLGTFQKTAGWEEIFSTPNTRLLKIS"
     gene            complement(7500..8927)
                     /locus_tag="P5079_04085"
     CDS             complement(7500..8927)
                     /locus_tag="P5079_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29199.1"
                     /translation="MKTLFFLFLLLTTPGSRLYAQKDEVPYRRVVAALHLHSNISPNG
                     RTSLQAIASLAGKRGIDALIPADTFLQRWEYGMPPLPGLLRATHYRKCVLKFGPEPYL
                     SLLHKLQTDFPKLTVVPGLEVAPAYRWTGHPAKGNLTLHEPQRHILVLGLENPEDIRR
                     LPVLSNPAVGDVRWKNLLLPALLVAAGVALMFRWPAGLIVVVFGLLWGVDQRPFRRPP
                     EAYTAFSRNGYAAAQHLIDYVNARGGLTVWAHPEASNWQQPQKLYRSVLMQTEPYPES
                     LLETRDYTGFGYFWEGEKIVGSPGGTWDQSLLEYALGKRENPVWAFGELDWSEEGRLG
                     TRMDSVQNVLWVKDATKEAVLDCFRRGRFYCVLQQKGHSLVMSDWTVESGKRLGLAGE
                     TVEWIPDAKLRARVAVEEPEGSHTLYVTLVRNGKIWKQFRESKTWRAEIPLPPPAGAG
                     RPDYYRLLVKEEGHSALAANPIFVN"
     gene            complement(8924..11176)
                     /locus_tag="P5079_04090"
     CDS             complement(8924..11176)
                     /locus_tag="P5079_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY29200.1"
                     /translation="MTFYSFDTDTPKSAPPPCWVCGAPDRRPVPLPNGAVALSCPECG
                     LVHRWPLPSDEEVFRRLGRPGPVDAQEMERQRTSKETLRRWELRRVQELSPVGRLLDV
                     GSGYGFFLDLARQEGWETEGVEISHPHWRYATEKFKLSVQNRPLTQTHFSPESFDVVT
                     LWEVAELLADPWAVFNEVFRLLKPGGWIFLRSNNALFQLPALRIANHPLLRWLHLRPG
                     LLHPFGLSPRPLSRALQDRGFVHVTHVPSPTTTGDPYRQGGWLGGLGVAAGKAAVRTA
                     ARTTFRLSGKIISSTFITRAQKPFPRPLVMHLITRLDPGGSAENVVLSTEKTAPDRCS
                     ALLISGKTGKTPGTAPRRVLSVPALRRSISPREDARAFLTLLRIFTAYRPALVHTHSS
                     KAGFLGRWAARFAHVPRIVHTPHGHVFYGYFSAGKSRLYALLEKITAKITHRLVALTP
                     TEKTEALRWGVGEDRQWRVVHSGVAWDKQYVEQKRLFREKMRQKLNVPQDALVVGFVG
                     RLEPVKGARVLALAAEEWLRACLDRRFPSENLDAGPMPNPNLASLTRRACGTNLAQAE
                     VRQPNLQAGNGLSEQALKTAGSAKAYFLLVGEGPERVFLDRIRARLPEPARLVLPGHQ
                     QEPMLYMAAMDIYVQPSLNEGMGKTLVLAQAMGLPVIASEVCGIPDVLRKNESGLLVP
                     PGDPHALALAIEKLMGAPDLREKFARAGEKWVNDAVDGGPRFSVERMTNLLEKVYEEL
                     GLFDGSGGKP"
     gene            complement(11173..12351)
                     /locus_tag="P5079_04095"
     CDS             complement(11173..12351)
                     /locus_tag="P5079_04095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29201.1"
                     /translation="MNLLITSDSFFPEEPGGSARVPWEIARRLVQRGHRVQFLVRRAP
                     DAPSFEDREGVKIISCRCRRQNILRSFWAARKAAQRITFPLDAVHIHHPFTGAAALLG
                     GALRDLPKIYFFHSPWHQEYLIRKKFFKNPSPIEKLAAWARRRVESFALRRCDRILTL
                     SQFMTNQLRSVHRVPAEKIDRISGAADIQRFSPRLSVPDARRALQWPLDKTLLLTVRN
                     LEARMGLENLLEALRRLRATDPSLHLHIVGQGSLRPFLEEQARRLGLQNHVTFLGFVP
                     DEALPLCYQAADIFVLPTRELEGFGLVTVEALASGCPVVATQVGGSAEILKPFDRSLM
                     ALGSSADDLLLPLQILLKNRGRWPQLRAECARYAAQKYSWDAVVGLVEKTTLALLKNP
                     "
     gene            complement(12414..13646)
                     /locus_tag="P5079_04100"
     CDS             complement(12414..13646)
                     /locus_tag="P5079_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29202.1"
                     /translation="MKPAPPPQPQPRPSTPTDATLRAPLHVLHVAGSAQWGGGEIFLQ
                     QMAEHLDRSRFRMHVLCPEEGPLKKELTDRGVPCTTVPLSPLGDPRPLRRLSSMFREW
                     KADVVQSHGARANFYTRLAAGKIPNISTVHNALSDYPVHPLKKTLYLTLDNFSAGKAS
                     AVVFVSESLRKEFLDRAPRWKEKTRVIHNGVDLTRFDPAKKPPEKLRAALSLKPVWTL
                     GIIGRMTPQKGHIYLLEALRRALPLLPTFQLLIVGDGLLRPWLENTVTQYGLRPYCHF
                     LGVRRDIPELLSLMDVLVVPSVSEGFPYVVIEGLALQRAVIATRVNGIPEILRADRDG
                     YLVPPRQPEKIEEALYAILKNPAEAKARGENGARRVREEFDARKAIGRWEALYLELTR
                     GRENERPPANTEISDIKY"
     gene            complement(13643..14869)
                     /locus_tag="P5079_04105"
     CDS             complement(13643..14869)
                     /locus_tag="P5079_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29203.1"
                     /translation="MNICLLTKTTSAHGWGGVEVHVQMLRRRVPESGHSLTVIASRLP
                     DGAPMERTPHGDVHYLPETTPGRYNRAFWTAVERKFRELHAAKKFDVVWGEDFAGYSY
                     AFATRRHRPAPLLSFLHGPTFRGRLESERRRLDSFVEWARFAAVYVPESFLFYRPWYA
                     ETLAHSDKLLAVSPQAAERYRREYGVPGDKISVLPDGVDTLLFKPEAGRRARCRKRYE
                     LADGTPVLLMAAVVHKQKGMHVGLKAFGALKKDVPGAKLLVAGDGPHLSELRSLSERM
                     GLKDDVVFCGRVPNDEMPDLYNAADIFLNPTLRWEGLTITTLEALACGKPCIVSRCGG
                     TESTLEDKVSGFFVPPNDPVSIAVHGVELLRDRTLYDRMSRNARQRADTHFNEEKIVQ
                     ELLSLSRQLAERGSPA"
BASE COUNT         2583 a         4244 c         5019 g         3139 t
ORIGIN      
        1 acggtgatga ggatctccca gagatgctcg ccggggaccg acggcgggac gaacgtgatg
       61 tgcacgttga agaacaccag gatgagggcc gccgccactt gggccgccag gcggaatttg
      121 gcggaaaggc cgcgcgcgtc gtcgatgagg ccggtgacga agatgagcgt tccggcggtg
      181 atgaggccgg tgagttcgcg ggaaaactgg aaattccggc ccaccgtgag cagcaccgac
      241 aaaaagatcg ccagcccccc caggcggggg atgggccgcc cgtgcgtttt gcgcgcgtcg
      301 gggatgtcga gaatatcgaa ccggtgcgcc agcgcgatgc acagcggcgt cagaagggac
      361 gaggcgagga acgccagcag caggacataa agccagcgca ggccccactc ccaggatttc
      421 gccgacagcc agccgggcgg gaggaggagc gccagcagga ccaggaggac cgcgatgtgt
      481 cccgcccgga gatgtccgcg gatgtttttg atgagaggga tcatttccta gagggcgaag
      541 tccagcgcca gacggacggg gacgaaacct tcggcatatt tgacccggtt ctggaacccg
      601 cggaagatgg cggccgagcg ggcgttttct atgatggaga gtcccggcac cttggtctga
      661 tacacctgcg agtggtgcgc tttcagcagg gcgaatttct tttcgatgtg tttcccgatg
      721 tccacgaaga ggttcggcgt gaagtccacg ctgctgggga cctcgtagaa aagaacgttg
      781 cgcacgtagc gcgtggcggt gacggtggcc tgcgagacgt tgcggtggtc ctggtgggtg
      841 tcgttgtggt agggcacgaa gatgaggagg ggctgggtgt ccgcgatgac cgcctcgacg
      901 cgatcgatca gcgctttctc cagcggcacc tcggtgtcct tccagctccc ccagtggagg
      961 cgggccttca gaagtttggc ggatttttcc tgttccttct cgcgcaccgc gggggcgccg
     1021 cccatgccgc ccttggtgat gacgagcagg tggatgcgat gccccttgtc cgagagtttg
     1081 gcgagggttc cgccgcaacc gtattcgagg tcgtcaggat gaggtccgat ggccagcacg
     1141 ttcatgagag gtccccttcg attttttatc cggccccgcc gaggctttcg atggcttccc
     1201 gggcgatgtc ccggtccacc gcggcggcgt ttttgctgta gcccgtgagg aggctgacgt
     1261 cgcaaatatg gttgatgcgc cgggggatgc cccccgagcg ttcgaaaatc acgtgcacgg
     1321 cctccggcgt gaagacgggc cgggccgacc cggccacctg catgcgatgt tcgatgtagc
     1381 cccggacgtg ggcggcaccc agggggtcca attggtaacg catggccagc cgctgggcga
     1441 gctgtttgtt gctgtccacc ttgggctgaa gttccggctg gcccaacaga aagagggtga
     1501 ccaggaactt ttcttccgtc tgaaagttca gcaggaggcg cagctcctcg aagaggcgca
     1561 cgtcctcgat ggcgtgggcc tcgtccacca ccaccacggt gcgccggccg tcccggaggt
     1621 tctcgtcgaa tattttttcc agggcgacca gcacgtcggc cttgcgggtg ggcaggtccg
     1681 gcaggcccag cccgtgcgcc accatgcgca ggagttccac gtcgtcgagg cgcgggttgg
     1741 tgatgtaggc cacgcggtac acgtccttct ccaattcgcg cagcagcgtg cgcccgagga
     1801 gcgtcttgcc gcagccgtag acgccggtca gcatgcccgc gcctttcccc tcccgcacca
     1861 cgtaaagcag gcggctcaac gcctcttcgt gctgcgggtc gttgtagagg aagcgcgggt
     1921 cgggggtgtt ctcgaagggc ttgatcgtca aaccccaata agcttcatac atggtgttcg
     1981 tccctcagtt cttcggcaaa aggatgttcc ggctttccgg tccgcagttc aggagcaggt
     2041 ccagggccgc catgtacggt tcaaagcccg ggtaggcctg cggatattcc ggatgcttga
     2101 attcctgata gatcactttc aatcccgctc gggcgaagag ggactcgtcc agatagtccc
     2161 tcgcgccttg gccggagaga taggtgtccg cgcccaccgc cagacagaga gcgaccagcc
     2221 tttcggtctt cttggaccgg acgtggaact ccgacgagcg gacgatcggc gtgtcgatct
     2281 ccagaaggcg gcggtggaac tcgatgaggg cctcgctcat ctccgccagg cccccccatt
     2341 ttttccgata gaacgtctcc aattcgccgg catggctctt gaagaagagg gctttggaat
     2401 aggccaggga gaccgccgtc caatgatctt tgccccagtc cgaggtggga tcgatggcca
     2461 cctcgcggat ctcctgatag taacaattct tagtaacaac gggaaccgtc agccactggc
     2521 ttccgtcttt gacccggatt ttgttcctgt tttgaaattc ccgtttcttg tattggacgt
     2581 cgtccagata gacgtagacg tcgcaggagg ccattttatg gaaatagccc agccaaggga
     2641 ggtactgtgg ctggtgcgcc gccataatca tgggcggaat tataacaaat atggcatgga
     2701 cagtagaact tcgaaaaaca aacttatgca tacaagaaga tgtgaaaatg atttagaata
     2761 attgcgcccg tcgcgcccga gcggcggggt tttaaaggag aacggatgga aaattcaaag
     2821 acgttcatta tccccgttcc cggggattcc ttgaccgggg ggccggaggc cctgcatcaa
     2881 ttggcccggg agatcaacga tcttccggat tgtcaggctt tcatccacta cacggggcga
     2941 aaggatcccc gggtcgcgga gtaatatcgg cggttgtacg ccacccggga gacggccgat
     3001 ccgcgggaag atggggcggt gctggtcctg ggcgaactct atgacccgag cgagtggaat
     3061 ttcccggagt tcaaagcgaa gtgggtctgg tggttgtctt gtcggcgtca ctatccgctg
     3121 gaggcgtatc ggggatgggg ccacctgttc caatcggaat acgcccgcct ccttttcgaa
     3181 aaaatcggac tgtcgggttt catgctgacc gaccacatcc gcccggattt tttcgaatcc
     3241 ccccccgccc cctcctccaa aaaagacctc atcattttca gcggctctcg cagcgcgctc
     3301 aatgtccttc ggttgaaatt gacggagcct cggttgaggc tcatgccctt gcgcgggctc
     3361 ggtgcgccgc agatcgttcg gcggatggat gaagccaaga tctatctgga catggggctg
     3421 cacacggggc gggaccggct gccgcgagag gccgccttgc gcggctgtct ggtggtgatg
     3481 ccgcgtcagg gcgcggccgc cggcccggcg gatgtcccga tcccggacga gtatcgatgg
     3541 ccggccggcc gggtggcggg actgggagat ttcctgcggc gtctcttaaa ggaatacgac
     3601 gagcgagtcc gggatttttc cgggtaccgg gactggatcg ggaatcagcg ggccgcgttt
     3661 cagaaagagg tgcggcattt ttcggaagtt tgccgcgacg ttcgtccccc cgccgtctcg
     3721 ccggcggaga agatattgcc tcaactcgat gcgcaggtcc tggaattgga ggagtcgctc
     3781 ggccgtctga agcgggagtc cgccgatctg gccgtccgcg tggagaacgt tttgatgctc
     3841 atgccgctga aggtgcgcct ggccgccctg ctgaacgcgg cggttggccg gttgagacgc
     3901 ccgcgatgaa gtcggcggcg gggccgggcg tttgtgtgga tttggggtgt gggaagaaca
     3961 aacagcccgg ctgtttcggg gtggacgccg tccttctgga cggcgtggat gtcgtgggcg
     4021 atttgtcccg gggaaacatc cctttccgca cctcttccgt gcgtggcgtt tattgttccc
     4081 attttttgga gcatctggag gaccccgggg ccttgctgaa agaaatccac cgggtgcttg
     4141 tcccgggcgg gcggctcttc ctccgcgtgc cgcattacag caattacgcc gggttcaatc
     4201 ccgcccatcg gagttttttc gggcagaact tcttcgacat ttacgatccg gacacggatt
     4261 tgggaaagcg gttcgactat ttctccgacg aaaagttccg gatcgtcaat aaggaattgc
     4321 ggcttttcaa aacgtggaag ttgccctaca cctacctcct gcagtggctg gcttcctgga
     4381 agccggccgc gtacgaatgc catttcgctt tcctttcccc cgccgaagag atctatgtcg
     4441 agatggaagt cctaaagtag atgggcgtcc ccctttccaa gaccatcacc cgcaacaccc
     4501 tgtattcggc gtatctcacg gcgttgcagt tcgtcgtcgg actggtcatg gtgccgtacc
     4561 tggtgtttcg gttggggctg gattatttcg ggatatggtc caccgtcttc gccgtggcgg
     4621 gctatctcgg attcatcgac ctgggagcct ccacctcgtt cgtcccctat ttcgccgcgg
     4681 cggccgcccg gaaggacgag caggagttca accgggtttt caacaccggg ctcctgttct
     4741 acggcgcggc ggccctgatg ctgaccctgg ccgcgccttt ccttgccggt gaacttttgg
     4801 acctcttgaa aattcctccg ccccttcggg gagacactcg gttcgccctc acgtgggcca
     4861 tttggatcta cgtgtatgga ttgtgggcgg gggtgttccg gtccgtgttg gtcggttttc
     4921 agcggatgga catttccaac cggatcgaga tggggctggc gctggcggcc tcggccgggg
     4981 cggtgttggt gctggaactc ggtttcggct tgagggggct gatcttgagt cgggcggtcg
     5041 tggtgttcgg cgcgtccctg ttcacccttc gggcggcgct gaagatggaa ccgatccgtc
     5101 cacgattttc cccccgtctt ttttccgtcg acatgttgaa aaaatgtttc gcttacggcg
     5161 tcaacacgca gatcagttat ctgtgcagca tgggcacgtt gtatctggcg aacatcgtga
     5221 tcgcgcggta tctgggcctg gccgcggtgg gcgtctatga gatcggccgg aagatcgcgc
     5281 agactgcgcg gagtttgccg atggtgttgt tgccggcgct ggtgccggcg agcgcggacc
     5341 tgtacggccg ttccgacggc gacaagttga tgcgtttgta ccgaaccggt tccaaataca
     5401 tgtcggcggt caccgtccca ctggcggcat tcctggtttt tcagatggag agaatcgtcc
     5461 gggtctgggt gggtccggga tacggccctt ccgtgctcat cggtcaagtc ctcgtggcgg
     5521 cgtatctgtt caacatgttg acgggcgttc cggggtccat cgcccgtggg atcgaccagg
     5581 tgcggctgga agcccgctct tccaccgtgg tgttgtttct gacggtgctg ttcatggggg
     5641 cggccgtgag gagcgatgcc gggttggcgg ggttcgccgt cgcgatatgg ctggccatga
     5701 gcacggggac cctctatttc atcgttttgg cgcaccggaa gatattccat atcccggtcg
     5761 gtcctttcct ggcgcagatg ctgcttaagc cgtcggcgtc gtgtctggcg gcaggggccg
     5821 tttccgccgg cctcttttgg atttttccgc cgcccggggg ccgtttcgcg gaaggtctcg
     5881 cgttggcgac ggttttttcc gtttttgcgg cggccttcat gctcctgctg catttcgtga
     5941 gatatttcga tgcggaggag acgcggatta ttttttccgc ctggaggcaa ttgaaatcgt
     6001 cggaccgccg gtgaaagcgc gcccgatttt ttcggccggt ttttccctcc tgtccggcat
     6061 ttttgttttc ctgtgtctgg cgaagctctg catggaaccc atgatgctgg ggcgggacag
     6121 cgccatttat ttcctcgccg cccgctccct cctttcgggg cacggctatg tgctggagat
     6181 cggcggcccc gccctccaac cgttgtatcc tcccgggtat tcgctcttcc tgagcctgtt
     6241 cgtgtggact tttccgaaac atgtgtgggt cttgcggttg acctccctgt tgccctgttt
     6301 gggggcggcg ctgattttat ggaaatggtt cgggaaaagc cgccgtggcc ttcttttttg
     6361 tgggatattt tttttcaatc cggtgacgct cgacctggcg ctggcggtgc ggccggagat
     6421 gttttttata ttcctggtct ccttgatttt atggttggca agggacgacg tgttcggcgg
     6481 ggaacgagga tatttcggtc gcttgggcgg tgtgttgttg ttggcttgtc tgtcggttta
     6541 tgtcaagacg gcggggatcg cccttgttct ttcggcgggt ctctgcgttt ggctgtcccg
     6601 gtggaagata tcccataaac ttttggccac ggcattcatg gcggcgcttt tatccccctg
     6661 tttttttgcg cagtcaaaag attatgtggg ccgctacttt tttcaatcgg acatcgtggc
     6721 cgacaaagtc gctttcgggt ggcgcccctt catcgaaagg atttccggga acgtggcgat
     6781 ccatctcggc aggaacatgg cggattttgt cttcggcggt gtttttcaaa acatcacgag
     6841 agccgcgtcc gggtccttct tttttggggc gaagaccgcg ctcagcctgt ttgtgggagg
     6901 gttggttctg gcggggtggt ggaggtccct ccggcggaga accggattgg ccggcctttt
     6961 tttcattttc tacacggggc tcgtgttgtc gtggccgtgg tataaaatgg aataccggtt
     7021 cctttttccc ctcttcccgc ttctcttcta ttatttttgg gcgggcgccc gcggtttgat
     7081 gcctcgcgcc gtgttcggca aggcgattct cgtctgtttc atgggcgccg ccgttttttt
     7141 ctcccttttc caggccgtct ggtttgtggg cgtccggaag atgcgggcac cctccgtctg
     7201 gtcggacgtc gtttgggcgc agaggacgcg ggagtttttg gacggcatgg agtggattcg
     7261 aacgcatgcg gagaaagacg ccgcgctgct ctgcggagat ccttacgcgg tgcatctgta
     7321 cacgggaagg aaatgtttcc tgtttcctcg ggcggtttcc gtgtcggagc tgcccggcga
     7381 ggccggctat gtctacgcga cagcgggacg aaaagacgag atggattttt tagggacttt
     7441 tcaaaaaacc gccggatggg aagaaatctt ctcgacgccg aacacccgct tgttgaaaat
     7501 cagttgacga agatcgggtt ggcggccaac gcggagtgcc cctcctcttt gacgaggagg
     7561 cggtaatagt ccggtcgacc ggcgccggcc ggcggcggca aggggatttc ggcccgccac
     7621 gttttactct cccgaaactg tttccaaatt tttccgttgc gcaccaaagt gacataaagg
     7681 gtgtggctcc cctccggttc ctcgacggcc acgcgggccc ggagtttggc atcggggatc
     7741 cactccacgg tttcgccggc taacccgagc cgtttgccgc tttcgacggt ccaatccgac
     7801 atgaccagcg aatgtccttt ttgttgcagg acgcagtaaa accgtccccg ccggaaacaa
     7861 tccaacaccg cttccttcgt ggcgtctttc acccacagca cgttttggac ggagtccatg
     7921 cgcgttccca gccgaccttc ctccgaccaa tccagttccc caaaggccca gacgggattt
     7981 tctcttttgc cgagggcata ttcaagcagg gactggtccc acgttcctcc cggggatccc
     8041 acgatttttt ccccttccca gaaatagccg aacccggtgt aatcccgggt ttccagaagc
     8101 gattccggat agggttcggt ctgcatgagc acggagcggt agagtttttg cggttgttgc
     8161 cagttggagg cctcggggtg ggcccagacc gtaaggcctc cgcgggcgtt gacgtagtcg
     8221 atgaggtgtt gggcggcggc gtagccgttg cgcgagaagg cggtgtacgc ctcgggcggg
     8281 cggcggaaag gccgctggtc cacgccccag agcagaccga agacgacgac gatgagtccc
     8341 gccggccagc ggaacatcag cgccactccg gcggcgacga gcagagccgg gagaagcagg
     8401 tttttccagc gaacgtcgcc gacggccgga ttggacagga cgggaagacg tcggatgtct
     8461 tcggggttct ccaagcccag aaccaaaata tggcgttgcg gttcatggag ggtgaggttt
     8521 cccttggccg gatgaccggt ccaacgatag gccggggcga cctccagccc cgggacgacg
     8581 gtcagttttg ggaaatccgt ttggagtttg tgcaggaggg agagataggg ttccggcccg
     8641 aatttcagga cgcacttccg ataatgggtg gcgcggagga gtccgggcag gggcggcatg
     8701 ccgtattccc agcgctgtag gaacgtgtcg gccgggatga gcgcgtcgat gcctcttttg
     8761 ccggccaggg aggcgatggc ctgcagggag gtgcgtccgt tggggctgat gttggagtgg
     8821 aggtggagcg cggccacgac ccgccggtag gggacttcgt ccttttgggc ataaaggcgc
     8881 gatcccggcg tcgtcagtaa gagaaaaagg aaaaataatg ttttcacggt tttcccccgc
     8941 tgccgtcgaa aaggccgagt tcctcgtaga ctttttccag gaggttggtc attctctcca
     9001 cgctgaagcg ggggcccccg tccacggcgt cgttgaccca tttttcaccg gcgcgggcga
     9061 atttttcccg caggtcgggc gcccccataa gtttttcgat ggccagggcg agcgcgtggg
     9121 gatctcccgg cggcaccaga aggccgctct cgttttttcg gagcacgtcg gggatgccgc
     9181 agacctcgga ggcgatcacc ggcagaccca tggcctgcgc cagcaccagc gttttcccca
     9241 tcccttcgtt cagcgagggc tggacgtaga tgtccatggc cgccatgtac aacatcggtt
     9301 cttgttgatg gccgggcaga acgaggcggg cgggttcggg gagtcgggcc cggatccggt
     9361 ccagaaagac cctctccggg ccttcgccca cgagaaggaa ataggctttg gcggaaccgg
     9421 cggtcttaag ggcctgttcc gataggccat ttccggcctg caaattcggc tgccggacct
     9481 ctgcctgcgc caaatttgtc ccacatgctc gccgagtaag actggccaaa tttggattcg
     9541 gcataggccc agcatccaaa ttttcgcttg gaaagcgcct atcgagacag gctctaagcc
     9601 attcctccgc cgccaacgcc agcacgcgcg cgcctttcac gggctccaaa cggccgacga
     9661 accccaccac cagggcgtct tggggcacgt tcaatttctg gcgcattttt tcccgaaaaa
     9721 gccgtttttg ttcgacgtat tgtttgtccc aggcgacccc gctgtggacc acgcgccatt
     9781 ggcggtcttc ccccacgccc cagcgaaggg cctccgtttt ttccgtcggg gtgagcgcca
     9841 ccaggcggtg tgtgattttg gcggtgattt tttccagcag ggcgtagagg cggcttttcc
     9901 cggcggaaaa ataaccgtag aagacatggc cgtgcggggt gtggacgatg cgcggcacgt
     9961 gggcgaagcg ggccgcccac cggccgagga atccggcttt ggaagagtgg gtatggacca
    10021 gcgcgggccg gtaggcggtg aaaatacgga gcagggtcag gaacgcccgc gcgtcctccc
    10081 gtggcgagat ggaacgccgg agggcgggga ccgacagaac gcggcggggc gcggtcccgg
    10141 gcgtttttcc cgttttcccg gaaatcagca gggcggagca gcgatcgggg gccgtttttt
    10201 ccgttgagag gacgacgttt tccgccgagc cgcccggatc gagccgggtg atgaggtgca
    10261 tcaccagcgg ccgggggaag ggtttctgcg cgcgggtgat gaacgtggac gagatgattt
    10321 ttcccgaaag gcggaacgtc gttcgggccg ccgtccgcac ggccgctttc ccggccgcga
    10381 cgccgaggcc gccgagccac ccgccctggc ggtaggggtc gccggtcgtg gtgggagacg
    10441 ggacatgcgt cacgtgaacg aacccgcggt cttggagggc gcggctgagc ggccggggag
    10501 agagaccgaa cgggtggagg agtcccggcc gcaggtgcag ccaacggagc aacgggtggt
    10561 tggcgatccg cagggccggg agttgaaaaa gggcgttgtt ggaacgcaaa aagatccagc
    10621 cgccgggttt gaggagccgg aaaacctcgt tgaacaccgc ccacgggtcg gcgagaagtt
    10681 ccgccacctc ccagagggtg acgacgtcga aactttccgg cgaaaaatgg gtctgggtca
    10741 aagggcggtt ctgaacggac aatttgaatt tttccgtcgc gtatcgccaa tgaggatggg
    10801 agatctccac gccctccgtc tcccatccct cctgccgggc gaggtcgagg aagaaaccgt
    10861 atcccgagcc cacgtccagg agccgtccga cgggcgaaag ttcctgcacg cgccggagtt
    10921 cccagcgccg aagggtttcc ttgctggtgc gctggcgttc catttcttgt gcgtcgaccg
    10981 gcccgggccg gccgagcctc cggaaaacct cctcgtccga cggcagcggc cagcggtgca
    11041 ccagtccgca ttccgggcac gagagagcca cggcgccgtt gggcaggggc acggggcggc
    11101 ggtcgggggc gccgcagacc cagcagggcg gcggggcgga tttcggcgtg tcggtgtcga
    11161 aggaataaaa ggtcacgggt ttttcaagag cgcgagggtc gttttttcaa ccaaaccgac
    11221 gacggcgtcc caggaatatt tttgcgcggc gtagcgggcg cattcggccc gaagttgcgg
    11281 ccagcgtccc cggttcttca acaggatttg tagcgggaga aggaggtcgt cggcggagga
    11341 gcccagggcc atgagagaac ggtcgaacgg tttcaggatc tcggcgctcc cccccacctg
    11401 ggtggccacc acgggacatc cgctggccag cgcctcgacg gtgaccaggc cgaacccctc
    11461 cagttcccgc gtgggcaaaa cgaagatgtc cgcggcctgg tagcagagcg gcagcgcctc
    11521 gtccgggacg aaaccaagga acgtcacgtg gttttgcagg ccgagtcggc gcgcctgctc
    11581 ttcgaggaaa ggccgcaggg acccttgccc cacgatgtgg agatggaggg aggggtcggt
    11641 ggcccggagc cggcggaggg cctccagaag gttttccagt cccatgcggg cctccaggtt
    11701 ccggacggtg aggaggaggg tcttgtcgag gggccactgg agggcccggc gggcgtcggg
    11761 gacggacagg cgggggctga accgctggat gtccgccgcg ccggagatgc ggtcgatttt
    11821 ttcggccggc acgcggtgga cggagcgcag ttggttggtc atgaactggc tgagcgtcag
    11881 gatcctgtcg cagcggcgca aagcgaaaga ttccacccgc cgtctcgccc aggcggccag
    11941 tttttcgatg ggagaggggt ttttgaagaa tttcttgcgg atcaggtatt cctgatgcca
    12001 gggggaatga aaaaagtaga tcttgggaag atcgcgcagc gctcctccca ggagggcggc
    12061 ggcgccggta aaagggtgat ggatgtggac ggcgtcgagc ggaaaagtga tccgttgggc
    12121 ggcttttcgg gcggcccaaa aggagcggag gatgttttgt cgccggcacc ggcaactgat
    12181 gattttaacg ccttcccggt cttcaaagga aggagcgtcg ggggcccggc gcacgagaaa
    12241 ctggacccgg tgtccccgtt ggacgaggcg ccgggcgatc tcccaaggga cgcgggcgga
    12301 cccccccggt tcctccggga agaagctgtc gctggtgatg agcagattca tgaggaaagc
    12361 cccctattga tataacaaga aatcgttaat tatcaacaac tattaaaata tttctaatat
    12421 ttaatatcag atatttcggt gttggccgga gggcgttcgt tctcccggcc cctcgttaat
    12481 tccaaataaa gcgcttccca ccggccgatg gccttccgtg cgtcgaactc ctcccgcacc
    12541 cggcgggccc cgttttcccc ccgcgctttc gcttcggcgg ggttcttgag gatggcgtag
    12601 agcgcctcct cgatcttttc cggctgtcgc ggcggaacga ggtagccgtc ccggtcggcg
    12661 cggaggatct ccggaatacc gttgacccgc gtggcgatga ccgcccgttg gagggccaac
    12721 ccctcgatga ccacgtaggg aaacccctcg gacaccgaag gcacgaccag gacatccatc
    12781 agggaaagga gttcggggat gtcccgacgc acgccgagga agtggcagta ggggcggagc
    12841 ccgtattggg tgaccgtgtt ttccagccag gggcggagca gcccgtctcc gacgatcagg
    12901 agttggaacg tcggcaggag aggcagggcc cgccgcaggg cctccaggag atagatgtgc
    12961 cctttttgcg gcgtcatacg gccgatgatc cccagggtcc agacgggttt cagggaaagg
    13021 gcggcgcgga gtttttcggg cggttttttc gccgggtcga atcgcgtgag gtccacgccg
    13081 ttgtggatga cccgggtctt ctctttccag cgcggagccc ggtccaggaa ttccttccga
    13141 agggattccg acacaaagac cacggccgag gctttgcccg ccgagaagtt gtccagcgtg
    13201 aggtagagcg tttttttgag gggatgcacc gggtagtcgc tcagggcgtt gtggacggtc
    13261 gagatgttgg ggattttccc tgcggccagg cgcgtgtaga agttggcgcg tgctccgtgc
    13321 gactgcacca catcggcctt ccattcacgg aacatcgacg acaggcgacg gagaggccgg
    13381 gggtctccca agggggacaa gggcaccgtt gtgcagggaa cgcctctgtc ggtcagctct
    13441 tttttcagag ggccttcttc cgggcagagg acgtgcattc ggaaccggga acggtcgagg
    13501 tgttcggcca tctgttgaag aaagatttct ccccctcccc actgggcgct gccggccaca
    13561 tggagcacgt ggagcggggc gcggagggtc gcgtccgtcg gcgtggaagg acggggttgg
    13621 ggttgcgggg gaggggccgg tttcatgcgg ggctcccgcg ttccgcgagt tgacgggaaa
    13681 gggagaggag ttcttggacg attttctcct cgttgaaatg ggtgtcggcg cgttgccggg
    13741 cgttgcggga catccgatca tacagggttc ggtcccggag cagttcgacg ccgtggacgg
    13801 cgatggaaac cgggtcgttc ggcggcacga aaaaaccgct gaccttgtct tcgagggtgg
    13861 attccgtccc gccgcaacgg gagacgatgc agggttttcc gcaggccagg gcctccagcg
    13921 tggtaatggt gaggccttcc cagcggagag tggggttcag gaagatgtcc gcggcgttat
    13981 agaggtcggg catttcgtcg ttgggcacgc gaccgcagaa aacgacgtca tccttcagtc
    14041 ccatccgttc cgacagagat cgcagttccg aaaggtgggg tccgtcgccg gcgaccagca
    14101 gcttggcgcc gggaacgtct ttcttgaggg ctccaaaagc ctttaagccc acgtgcatcc
    14161 ccttctgttt gtggacgacc gccgccatga ggaggacggg cgtgccgtcg gccagttcgt
    14221 atcgcttcct gcatcgagcg cgccgtccgg cctcgggttt gaaaagcagg gtgtcgaccc
    14281 cgtccggaag aaccgagatc ttgtcgccgg ggacgccgta ttcccggcgg tagcgctcgg
    14341 cggcctgggg gctgacggcc agcaatttgt cggaatgggc cagtgtttcg gcataccagg
    14401 ggcggtagaa caggaatgat tccggaacat agaccgcggc gaaacgcgcc cattcgacga
    14461 acgaatccaa tcgccgccgc tcgctttcca gccttccccg gaaggtgggt ccgtggagaa
    14521 aactgaggag aggggcgggg cgatgcctcc gcgtggcgaa ggcgtaggaa tagccggcaa
    14581 aatcctcccc ccacaccacg tcgaattttt ttgcggcgtg cagttcccgg aatttccttt
    14641 cgacggcggt ccagaacgcg cggttgtatc tcccgggggt cgtttcggga aggtaatgga
    14701 catcgccgtg gggagttcgt tccatcggtg cgccgtcggg gaggcgcgac gcgatgaccg
    14761 tgagggagtg gccggattcc ggcacccgcc ggcgcagcat ctgcacgtgg acctcgaccc
    14821 cgccccagcc gtgcgcggag gtcgtttttg tgagaagaca gatattcatc gcttcatttc
    14881 cccgtcccgg ccatccgccg cgcccgtcgg agacagagga gggtgacgaa cacgaactcg
    14941 ccggccagag ccaggaaact caacgccagg aaaacgtcca ggcgg
//
LOCUS       DAOTFD010000041        14916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2762_length_14916_cov_4.317004, whole genome shotgun sequence.
ACCESSION   DAOTFD010000041 DAOTFD010000000
VERSION     DAOTFD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_14916_cov_4.317004"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="P5079_04110"
     CDS             complement(<1..470)
                     /locus_tag="P5079_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29204.1"
                     /translation="MRNTRYEIRNTKGIARKVRKIFPLFVFPISCFIFSPLFAASVTP
                     VYDLRFFGGQHFYNGDISALSGNANVLISPAVKFNDTWSVIPTYMGGYQGTRDVQELA
                     GGGTLFQDSTNQGFTVKTIYGLTPSVKLKGTAGARADFLRETKDENWGDGLFDY"
     gene            complement(467..2113)
                     /locus_tag="P5079_04115"
     CDS             complement(467..2113)
                     /locus_tag="P5079_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY29205.1"
                     /translation="MFLSAAFLISLLSPVAVLAESITVKSFRAPVEVQRVKIPNKWFP
                     ARVGLELTSGDTIRTGAGARADIRMGDGSKMILGSSSRLTVQEVAPSRVMGLDFGRVK
                     AWVKKLKGGNKFEVKTPIAAASVRGTQFEIGYDEEGKTGFLDVTEGAVALLKDGREVL
                     VPAGARMEFVPDSPLGEPVPARSQGSLDEREGIRREVGLGMSKEEVMSAAAEEMRLAE
                     YQEGKTLIDVNGARVRLEEYIIRRPVEIAAADRDKAFKLVVLNERDDRFDYFYYRGLF
                     NTALPEDLSVALKDINGKLGVDAPSYYLTAYEMGQSNTMDYIKDTASGGHLVKVTYDG
                     TNYILTDPANASNTRTVAKDEQTVLSGVTYHKIYDPVNDRFFSLTDAQYNAGDYNAAV
                     YDPSNDSFKYIASGDTYWRTRYNTYSHVINGASKQSYTPKTAGLNTLAIDLDATFTYA
                     GGTLFSFTETPSGSDVLHNRVSLYYGDGTKEVYDTYIISDEGVIGPVSAFNNMSSGSA
                     FKSELLKWNYEQVAGATEFNGRKIDLVVEPKILIKSGLIK"
     gene            complement(2249..3091)
                     /locus_tag="P5079_04120"
     CDS             complement(2249..3091)
                     /locus_tag="P5079_04120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY29206.1"
                     /translation="MLIDTHAHLNDKQFDADRDEVLKRAFDAGVGAVVEIADQESEWS
                     RARGLAERHPGRVWWTAGLHPHHAGKVEKDMAWRLRDAVKHPQCVAVGEIGLDYYRNP
                     VPPYDQQKVFFECAQAAAESKKPLVIHCRESDAASCAAQDDMLSVLKSFFTGAPGTAN
                     PPPGVLHCFQGNLDFARACLEMGFYIGVDGPLTYPNAAALREIIGEIPMDRLVLETDC
                     PYLAPQSRRGRRNEPSYIRETAAALAKVKDVSVEQVEQQTARNAEQLYRLGFSSASSS
                     NRTP"
     gene            complement(3192..4751)
                     /gene="metG"
                     /locus_tag="P5079_04125"
     CDS             complement(3192..4751)
                     /gene="metG"
                     /locus_tag="P5079_04125"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRY29207.1"
                     /translation="MKTFYITTPIYYVNDVPHIGHAYTTIAADVLARWKRLCGEEVFF
                     LTGTDEHGQNIERIAREKGVSEQVICDTNSLRFRELWKNLSIENDDFIRTTEERHAAG
                     VRVLWDRLKTAKTPAGNPAVFKKKYAGLYCPRCEAFKTEDELNQPGNLCPDHGLPCEM
                     TEEENYFFRLSDYADWFRGVMANKEMTISPESRRNEVMGVVQQGLQDFSISRARVKWG
                     IPIPDDPEHVFYVWVDALSNYITALGFAATGEKRALYERFWEKGADRLHLIGKEIIRF
                     HCLYWPAMLQAAGLPLPTRVFAHGWWTVEGEKMSKTRGNVVDPRAIVEEYGGPGIGVD
                     VLRYFVLREVPFGNDGDFSRKALLNRYNAELANALGNLLNRTLQLLEKNNEGKIPPPS
                     PFSGGTALENYQDAMERLAFGEALESALWYVNEGNLMMDREAPWKTVKTDREAALRTL
                     FKSVTLLKTAAILLSPFMPAVMGELWKQLGERESLETAGSRFLREGVVQFAPGQTVQK
                     GAPLFPRKEVK"
     gene            complement(4825..5886)
                     /gene="holB"
                     /locus_tag="P5079_04130"
     CDS             complement(4825..5886)
                     /gene="holB"
                     /locus_tag="P5079_04130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRY29208.1"
                     /translation="MPSSRATPALFESVSMPVSPGLKGTSFESVFGQEAACEILLSAL
                     RAVRVPHAYLFAGPEGVGKRTAALQWAKTLNCLSPVSPVRACGACGSCRKISALNHPD
                     VLWVNFEYQAHVLEEPVEKQRILKINTVREMIQTLHLKPLEGRVKVAVVDPADRLGAD
                     AAHALLKVLEEPPPQTHLILLAQDPSQLLGTIRSRCQWVRFRPLATELLATYLTARAP
                     DRSPEDISALAAQAEGSLSRALEILEQGAEMDFDWETAPLSELLLWCEQFRGRGSHRD
                     EEKGGESSPVRRAEDFLRRLLARLELELRRGAGDPEKVRRVLEALHQLKQNVSPQLVL
                     EVLLLKSRRQRRKKEQAIG"
     gene            complement(5886..6557)
                     /gene="tmk"
                     /locus_tag="P5079_04135"
     CDS             complement(5886..6557)
                     /gene="tmk"
                     /locus_tag="P5079_04135"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRY29209.1"
                     /translation="MRRGLFITLEGPEGSGKSTQTRLLCDFLRRKGLHVVHTREPGGT
                     SIAEAVRRILLHPSSRIAPMTELLLYEAARAQHIAEIIRPALCHGAVVVCERFTDATE
                     AYQGWGRGLDREEIRALNRIATGDLRPQLTLLLDVPVREGLARARSLDKELSVRARRT
                     RGGDRLERESLAFHRRVQKGYRSLARKEPKRFRLIRWEDGIENVHARLIREVVSFLKK
                     RGVRV"
     gene            complement(6831..7877)
                     /locus_tag="P5079_04140"
     CDS             complement(6831..7877)
                     /locus_tag="P5079_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY29210.1"
                     /translation="MRKFLKRLGRSVFPRDRRGFTFVEVLITATLIAILVMAVIPLFN
                     VTQQGYTSLEVNTVLSSGGQGALNKIQHRIVENKRLFGNTTADAAFLSRVQLGGAPAA
                     LTGSHMPLIVSTASISPSTSTFSAGNVGNCLFFASLGPVKDLDSITTSAGTTCTVRVD
                     IYNFHYYYVAIDTAAPQVIAGKTKRALWEWHSVDFADYQQLNNIADATKKLNVVKALN
                     TANVLYAWDTSQTAVTSAFYTLSSAGAIAAASSQNIPKSSAKNMIVLLRGTTTGGFRY
                     GISPNTDGLSTSDVVPRFATASGEYPGGFEVMIVGANSARQIFIRLVQMAQGSFKGYI
                     TREQIVLSTTRDLW"
     gene            complement(7883..11029)
                     /locus_tag="P5079_04145"
     CDS             complement(7883..11029)
                     /locus_tag="P5079_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY29211.1"
                     /translation="MRIRGRFPKNQRGFTLVEVLIALAILSIVALASMKAFWFISSQN
                     VEVNERSFAAQKAIQMMEELRGLISDTNSNIGVLDDYDDGSAYKTILTTRVDVTDPAD
                     PISGNDGLKYVRRVSVIQMPNETLARRVYVRVYKADTKEALAETVSVLRTILNQNLPT
                     QVYDVYVLVLENVPGWWVSLSTMKPMFDNIAQDLETRNPGLELRLHYITRLAYGRDPY
                     YTPYINESAANRADLADPQGVYFYPGNVNKPSVGDFLYYVPDSLAGRINVDGTVTNGA
                     SYSIADQYNHGARYPEEEAIYGAVVASATAAGVDPPEISYRMLLEKMNSSPADFLNSL
                     VINLHGELVPLPPLRNYSDAAKSTDTALNSANAPWARVVTHPEQLSYNANTAVNLRVY
                     PYFAWTPDSYATSAAISTVTVLLTSTQIPAGNITVRKCAGSDTSAYAWQLASDTTDYN
                     VSYPADGQTLITLWNSPLRHPPNGNFGLPTTDRLYGLEYIPSLLAATTYFVEGDRDLT
                     TNSGSVPKNTARWVIRISPSSLVPGRHTVETRIGLSTMTGTADNEPTNVSRTYVWVGQ
                     TPPVTEQYQFIGDARHMPYADVKTNLGFNWYFENFDTTDYPGFTNRADRWNSACNIDI
                     PRYFQILRGALMNSVSLWTSMTGYSFYYVGIGGEIGYDASNGFGSGIPVRRVLWVPAG
                     GASEGVDEITDAQTHPYMRVISRADDTWWGMHWLGELYPDYATATWIANGNLPVGTGN
                     YYRRRYNTLGYSLGDPQKRTNTRGAASFFNGNPTAGNSNWFDHQFRDGNGATLTSSGS
                     LVGQDFNFSLLPTMTASRPFLLNDTDTGSQPPEWSAATYQAARNTLSTVEAYYETNGY
                     TSTEDSSALIRISNSGNVCGMIMNGLSPQTTFGSAQISKLCMVNLLRGFMIMGSPYIT
                     TGNIPQLPLVTISSPTVTDEFINPSSIYCSWSEAWTRWDGVLYSTQTYPTGYVGTSTV
                     TYNLKYSSDNGASWKFVQTGGAADDGVYSSARALASPYTWNVTTLPEGSYLLRVEGYR
                     QDRRQHYTYHQRRVYIKR"
     gene            complement(11026..12261)
                     /locus_tag="P5079_04150"
     CDS             complement(11026..12261)
                     /locus_tag="P5079_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29212.1"
                     /translation="MRRFLKSAVGPLENSRGSVVVLIVMLIALLSFIPIGAELVKVAR
                     KNIKLQTNATVQADNVARAGLTDAVAWFKRQTSQPVGSVKYGMSYPDAAFYPRTSTNS
                     VTSDTIDESIGLVKEYRIGESNWLWARYEARRQPDPSSNSIDPHAVHDITDKRVDGFA
                     AGDGLAWYIESVGYVYRLRNSTVAFNVAPNEIVARSRVATEIRRLSLTVPAAAVRITS
                     RANGTVNANCRVSGGNNAAGAMYTSGTGGSWSAGTRTTGTPALQSTTTAISVQSVFGV
                     TDYDLKLMADYNVTSVNALPASYPSMALVYINGAATFNSSRPLRGGGVLIVNGNLTLD
                     SGSNTLFSGLLWVNGNLTVNGPAIISGTAVVTGSLTMNGTVDVAEIQYDSGILSSVRQ
                     QVGQYRENKSAFHVFSAQK"
     gene            complement(12481..13632)
                     /gene="wecB"
                     /locus_tag="P5079_04155"
     CDS             complement(12481..13632)
                     /gene="wecB"
                     /locus_tag="P5079_04155"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRY29213.1"
                     /translation="MKKILCVFGTRPEAIKMAPVVLALRARRRAFKTLVAVTAQHREM
                     LDQVLSLFRISADFDLDIMREGQTLTDVSVLGLRRLEPVLQKAKPDMVLVHGDTTTTL
                     IATLAAYYQKIPVGHVEAGLRSFDALNPYPEEINRRLADAVCALHFAPTAAARENLLR
                     ENVSPKGIFVTGNTGLDALRLALANIRAGRFRSPRGRFRRFEREKFVLVTAHRRENFG
                     KPLADFCRAVADVARRRPEVHFVYPVHPNPHVLGPVRKFLSGPRNVHLLPPLDYPEFV
                     LFLQAAHLVLTDSGGLQEEAPSLGKPVLVLRKVTERPEAVQAGTVKIIGTDTAVVRRW
                     VLRLLEDRAVYRRMANAVNPYGDGRAAGRIIEAISYYFQLSRTPPRPFH"
     gene            complement(13629..14045)
                     /locus_tag="P5079_04160"
     CDS             complement(13629..14045)
                     /locus_tag="P5079_04160"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRY29214.1"
                     /translation="MPKRVKILFLCMGNSCRSQIAEGWARHLGGFRLEVYSAGKKAVC
                     VQPRALRVMREKGIDISSQWSKTMDKICFKDMDYIITLCGDGSLCPDVPATVKRLHWQ
                     VDDPVPVRGTDAEQMDAYRRTRDQMELRVRDFLRAL"
     gene            complement(14095..>14916)
                     /locus_tag="P5079_04165"
     CDS             complement(14095..>14916)
                     /locus_tag="P5079_04165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRY29215.1"
                     /translation="AYRHRLMEGGDVVGLLSLKAQFVGILVGALVVLALGMWDDRKSL
                     PALPKLLVQIIAAYVAMVYGVRIAGLALPGLGFVDFPIFLSQIITLLWLLGFMNAVNL
                     IDGLDGLAAGLAAIAAGTFLVVCVLQGETKIVLFSKQLKLAAILSAAVLGASLGFLLY
                     NFHPARVFMGDGGALFLGFMLGAISIIGTLKTSAVLAVIIPVLVVALPVLDVAFAMFR
                     RFRSGQGMMQADRLHFHHRLLAFGWTQKEIVLLMYVVTLVLSILTILLTLFKGRV"
BASE COUNT         2798 a         4342 c         4897 g         2879 t
ORIGIN      
        1 tggtaatcga acagaccgtc tccccagttc tcgtccttgg tctcacgcag aaagtcggcc
       61 cgggcgcccg cggtgccctt gagtttcacc gacggcgtca atccgtagat ggttttcacg
      121 gtgaagccct ggttggtgga gtcctgaaag agggtgccgc cgccggccag ttcctgcacg
      181 tcgcgggtgc cctgatagcc gcccatatag gtggggatga cggaccatgt gtcgttgaat
      241 ttcaccgcgg gggaaatgag cacgttggcg ttgcccgaca aggcgctgat gtcgccgttg
      301 tagaaatgct gaccgccgaa aaagcggagg tcgtagaccg gcgtgacgga ggcggcgaac
      361 agcggcgaaa atatgaaaca cgaaatcgga aatacgaaaa gcggaaaaat cttccggact
      421 tttcgcgcga ttcctttcgt atttcgtatc tcgtatctcg tatttctcat tttatcagtc
      481 ccgatttgat cagaatcttg ggttccacca ccagatcgat cttccggccg ttgaactccg
      541 tggcaccggc cacctgttcg tagttccatt tcagcagctc cgatttgaac gccgaaccgg
      601 aactcatgtt attgaaagcg ctgaccgggc cgatgactcc ttcgtcggag atgatgtagg
      661 tgtcgtagac ctctttggtg ccgtcgccgt aatagaggga gacgcggttg tggagcacat
      721 ccgagcccga cggggtctcc gtgaaggaaa acagggtgcc gcccgcgtag gtgaaggtgg
      781 cgtccaaatc gatggccagg gtgttcaaac cggcggtctt gggcgtatag gactgcttgg
      841 aggcgccgtt aatgacatgg gaataggtgt tgtatcgggt gcgccaatac gtgtcgccgg
      901 aggcgatgta cttaaaggag tcgttggacg ggtcgtagac cgccgcgtta tagtcgcccg
      961 cgttgtattg ggcgtcggtg agactgaaga aacggtcgtt cacgggatcg taaatcttgt
     1021 ggtaagtgac gccggaaagc accgtctgct cgtccttggc cacggtgcgg gtattggagg
     1081 cgttggcggg gtcggtgagg atgtagttgg tgccgtcgta ggtcaccttg accaggtgtc
     1141 cgccggaggc ggtgtccttg atgtagtcca tggtgttgga ctggcccatc tcataggcgg
     1201 tcaaataata ggagggggcg tcgacgccaa gtttgccgtt gatgtccttg agcgccacgg
     1261 aaaggtcctc gggaagggcg gtattgaaca ggccgcggta gtagaaataa tcgaagcggt
     1321 catcccgttc gttgagcacc accagcttaa aagccttgtc gcggtcggcg gcggcgatct
     1381 ccacgggccg gcggatgatg tattcctcca accgcacgcg ggcgccgttg acgtcgatga
     1441 gggtcttgcc ttcctgatat tccgccagac gcatctcctc ggcggcggcg ctcatcactt
     1501 cctccttgct catgccgagg cccacttcgc ggcggatccc ttcgcgttca tccagggacc
     1561 cctggctgcg ggcggggacg ggctcgccca ggggcgagtc gggcacgaac tccatgcgcg
     1621 cgccggcggg gaccaacacc tcccggccgt ccttcaagag cgccaccgcg ccttcggtga
     1681 cgtcgaggaa accggtcttg ccttcctcat cgtagccgat ttcgaactgc gtgccgcgca
     1741 cggaagccgc ggcgatgggg gtcttgacct cgaacttgtt cccccctttg agtttcttca
     1801 cccacgcctt gacccggccg aaatccaggc ccatcacgcg gctgggcgcc acctcctgga
     1861 cggtgagccg gctggaggag cccagtatca ttttggaacc gtcccccatg cggatatccg
     1921 cgcgggcgcc ggcgccggtg cggatggtat cgcccgaagt caactccagc cccacgcggg
     1981 ccgggaacca tttattggga atcttgaccc gttggacctc gacgggggcg cggaacgatt
     2041 taacggtgat ggattcggcg agaacggcga cgggggaaag aagggaaatg aggaatgccg
     2101 cggaaagaaa cattccggaa aaacggcgga cgcgtgactg cgggggcgtt gaatagttca
     2161 tagaaatctc ggcattccaa gtgtacccat gtgccgggat tttgtcaatt gtgattttag
     2221 tcaagacttt ggtccggact ttagtcggtc acggcgtccg attcgacgaa gacgcggagg
     2281 aaaagcccag gcggtaaagc tgttcggcgt tccgtgcggt ctgctgttcc acctgttcca
     2341 cggaaacgtc tttcactttc gccaaagccg ccgccgtctc ccttatatag gaaggctcgt
     2401 tgcgccggcc gcggcgggac tggggcgcca ggtaggggca gtccgtctcc aagacgaggc
     2461 ggtccatcgg gatttccccg atgatttccc ggagagccgc ggcgttcggg taggtgaggg
     2521 ggccgtcgac gccgatataa aaacccattt ccaggcaagc ccgggcgaaa tccaagttgc
     2581 cctgaaaaca atggaggacc cccggcggcg ggttggccgt ccccggcgcg ccggtgaaaa
     2641 aacttttcaa aacgctgagc atgtcgtcct gggccgcgca agaagcggca tcggactccc
     2701 ggcaatggat gaccaggggt ttctttgatt cggcggcggc ctgggcgcat tcgaagaaga
     2761 ccttttgctg atcgtagggg ggaacggggt ttcggtaata gtcgaggccg atctctccca
     2821 cggccacgca ttgcgggtgt ttgaccgcgt cgcgcagccg ccacgccatg tccttttcca
     2881 ccttgccggc gtgatgggga tggagccccg cggtccacca cacgcggccg ggatgtcgtt
     2941 ccgccaaccc gcgggcgcgg gaccattcgg attcctgatc ggcgatctcg acgacggcgc
     3001 cgacgccggc gtcgaaagcg cgtttcagga cttcgtcgcg gtcggcgtcg aactgcttat
     3061 cgttcaggtg agcgtgggta tctatcaaca tggcgttcgg tgaaatggtg aaacaatgga
     3121 atggtaaaag acaaaattaa aaacagtcga aacgctctgc gattttctct attacgccgg
     3181 tcgccatttc actatttcac ttctttgcgt gggaacagcg gcgcgccctt ttgaacggtc
     3241 tggccgggag cgaattgaac aaccccctcg cgaaggaaac gcgaaccggc ggtttcgaga
     3301 ctctcccgtt cccccaattg tttccaaagt tcccccataa cggccggcat gaaagggctc
     3361 agcaggatgg ccgccgtttt caggagagtg acgcttttaa acaacgtgcg caacgccgcc
     3421 tcccgatcgg tcttcactgt tttccacggc gcctctcgat ccatcatcaa attcccctcg
     3481 ttcacatacc acaacgcgct ttccagcgcc tcgccgaacg ccagccgttc catcgcgtcc
     3541 tgatagttct ccaaggcggt ccctcctgaa aaaggcgacg gcggcgggat cttcccctcg
     3601 ttgttctttt ccaaaagctg gagcgtgcgg ttgaggaggt tgcccagcgc gttggcgagc
     3661 tcggcgttgt agcggttcag cagcgccttg cgggaaaaat cgccgtcgtt gccgaaagga
     3721 acctcgcgca gaacgaagta gcgcaaaacg tccacgccga tccccggtcc gccgtattcc
     3781 tccacgatgg cgcggggatc caccacgttg ccgcgggtct tggacatctt ttccccttcc
     3841 acggtccacc agccgtgggc gaacacgcgc gtgggaagcg gaagtccggc cgcctgaagc
     3901 atggcgggcc aatagagaca gtggaaacgg atgatctctt tccctattaa gtggagccgg
     3961 tcggcgcctt tttcccaaaa gcgctcatag agggcgcgtt tttcgccggt ggcggcgaaa
     4021 ccgagagcgg tgatgtagtt ggaaagggcg tccacccaca catagaaaac atgttccgga
     4081 tcgtccggga tgggaatccc ccatttcacg cgggcgcggc tgatgctgaa atcctgaagc
     4141 ccctgttgaa caacccccat gacctcgttc cggcggcttt ccggggagat ggtcatctct
     4201 ttgttcgcca tcacgcctcg aaaccaatcc gcgtagtcgc tgagacggaa aaaataattt
     4261 tcctcctccg tcatttcgca gggaaggccg tggtcggggc agagattgcc cggctgattg
     4321 agttcgtctt cggtcttgaa cgcctcgcag cgggggcagt agagtccggc gtatttcttt
     4381 ttgaagacgg cggggttccc ggcgggggtt ttggctgttt tgagacggtc ccaaaggacc
     4441 cgcacgccgg cggcatgccg ttcttccgtg gtgcgtatga aatcgtcgtt ctcgatcgac
     4501 aggtttttcc acaactcccg gaatcgaagg gagttcgtgt cgcaaatgac ctgttccgac
     4561 acgccctttt cgcgggcgat gcgttcgatg ttctggccgt gttcgtcggt gccggtgagg
     4621 aagaagacct cctcgccgca gagccgtttc cagcgggcca gaacgtcggc ggcgatggtg
     4681 gtgtaggcgt gcccgatgtg ggggacgtcg ttgacgtaat agatgggcgt ggtgatgtag
     4741 aaagttttca tggaaatcca gttcaaggtt tgacgttgaa agttaaaagt cccgttcccc
     4801 cctttaactc ttccctttca acttttaacc gatggcctgc tcctttttcc ttctctgccg
     4861 gcgacttttt aaaagcaata cttccagcac gagctggggc gagacgttct gtttcagttg
     4921 atggagggcc tcgagcacgc ggcggacctt ctccgggtcg ccggcgcccc ggcgcagttc
     4981 cagttcgaga cgcgccaaca agcgccgcag aaaatcctcg gcccggcgga cgggagacga
     5041 ttccccgcct ttttcctcgt cccgatggga accgcggccg cggaactgtt cgcaccaaag
     5101 caacagttcg ctgagcggtg cggtctccca atcgaaatcc atctccgctc cctgttcaag
     5161 gatctccaag gcgcgcgaca gggacccctc ggcctgggcg gccagggccg aaatatcctc
     5221 cggcgaacgg tccggcgccc gggccgtcag gtaggtcgcc aacagctcgg tggccagcgg
     5281 ccggaaacgc acccattggc agcgggagcg gatggtgccg agaagctggc tggggtcctg
     5341 ggcgagcagg atcagatgtg tctgcggcgg cggctcctcc aggactttga gcagggcgtg
     5401 ggcggcgtcg gcgccgagac ggtccgccgg gtccacgacg gccaccttca cccgcccctc
     5461 caacggcttg agatgaagcg tttggatcat ttcacgcacg gtgttgatct tgaggatgcg
     5521 ttgtttttcg acgggttctt cgagcacgtg cgcctgatat tcgaaattga cccagagcac
     5581 gtcggggtgg ttgagcgcgg aaattttccg gcagctcccg caggcaccgc acgcccgcac
     5641 cggggaaacc ggagaaagac aattgagcgt cttggcccac tgaagcgccg cggtccgttt
     5701 gccgacgccc tccggccccg cgaagaggta ggcgtgcggc acccggacgg cgcgcagggc
     5761 ggaaagaagg atctcgcagg cggcctcctg cccgaaaacg gactcgaacg acgttccctt
     5821 caaaccagga gacaccggca tggaaacgga ttcaaacagg gcgggcgtgg cccgagacga
     5881 cggcactaaa cgcggacccc tctttttttc agaaaagaaa cgacttcccg gataaggcgg
     5941 gcatggacgt tttcaatgcc gtcttcccag cgtatgagcc ggaaccgttt cggctctttg
     6001 cgggcgaggg aacgatatcc cttctgcacg cgacgatgga aagccaaaga ctcccgttcc
     6061 aaccggtcgc cgccgcgtgt ccgccgggcg cggacggaaa gttccttgtc caacgagcgc
     6121 gcgcgggcga gcccttcccg gacaggcacg tccagcagaa gggtcaactg cggccggagg
     6181 tctcccgtgg cgatgcggtt gagggcgcgg atctcttccc ggtccagacc ccgtccccag
     6241 ccctggtagg cctcggtggc gtcggtgaac cgttcgcaga cgaccaccgc gccgtgacat
     6301 aaagcggggc ggatgatttc ggcgatgtgc tgcgcgcggg cggcctcata gagcaggagt
     6361 tccgtcatgg gcgcgatgcg gctggacgga tgcaggagga tccgccgcac ggcctcggcg
     6421 atgctggtgc cgccgggttc gcgggtatgg acgacgtgga gcccttttcg gcgcaaaaaa
     6481 tcacagagaa ggcgggtctg ggtgctcttg ccgcttccct cgggcccttc caaggtaatg
     6541 aacaaacctc tacgcatggg cctctcgctc agccggggcg ctcacgaact ctcgcccgtc
     6601 gccgacggtc gcccgtgacc ggctccgccc cgcggagccg ccggtcgacg gcgacccctc
     6661 gggcccttcc aaggtaatga acaaacctct acgcatgata tcctcgctca acctcgttat
     6721 ctctctattt tttatcgggc ggccgccggt ccctttgagg agacgacgtt ccggggaaag
     6781 tcccattgaa gcaaaaatgc gacggggagg tcaaagcggc ggaggaccgg ctaccaaagg
     6841 tcccgggtgg tggaaaggac gatctgttct cgggtgatgt agcccttgaa actgccctgc
     6901 gccatctgca ccagacggat aaagatctgc cgggcgctgt tggcccccac gatcatcacc
     6961 tcaaagccgc cgggatattc ccccgaggcg gtggcgaaac gggggaccac atccgaggtg
     7021 gagaggccgt cggtgttcgg cgatatcccg tagcggaacc cacccgtggt ggttccgcgc
     7081 aggagcacga tcatgttctt cgcggacgac ttggggatat tctgcgagct ggcggcggcg
     7141 atggcgccgg cggagctcaa cgtatagaac gccgaggtga cggccgtttg ggaggtgtcc
     7201 caggcataaa gcacgttcgc ggtgttcagc gccttcacga cgttgagttt tttcgtggcg
     7261 tcggcgatgt tgttcaactg ctgatagtcc gcgaagtcca cgctgtgcca ttcccagagg
     7321 gccctcttgg tcttgccggc gatcacctgg ggcgcggccg tatcgatggc cacgtaataa
     7381 tagtgaaaat tatagatatc cacccgcacc gtgcaggtgg tgcccgccga ggtggtgatg
     7441 ctgtcgaggt ctttcacggg gcccagactg gcgaagaaca ggcaattgcc gacgttgccc
     7501 gcgctgaagg tggaggtgct gggcgaaatg gaggcggtgg acacgatgag cggcatgtgg
     7561 ctgcccgtca gagccgcggg ggcgccgccc aactgcacgc ggctcaagaa agcggcgtcg
     7621 gcggtggtgt tgccgaacag ccgtttgttc tccacgatgc ggtgctggat cttattgagc
     7681 gccccctgtc cgcccgaaga cagaacggtg ttcacctcca aactggtgta tccctgctgg
     7741 gtgacgttga agagcgggat gacggccatc accaggatgg cgatcagggt ggccgtgatc
     7801 agcacctcga cgaacgtaaa cccccgccga tcgcgcggga acacggaacg tcccagacgt
     7861 ttcaaaaatt ttctcatggg gatcagcgtt tgatgtagac ccgccgttga tggtaggtgt
     7921 agtgttgccg gcggtcctgg cggtagccct cgacgcgcag caaatagctc ccctcgggca
     7981 gggtggtcac gttccaagtg tacggggagg cgagggcgcg ggccgaacta tagacgccgt
     8041 cgtccgcggc cccgccggtc tggacgaact tccaggacgc cccgttgtcg gaggaatatt
     8101 tcaggttgta ggtgacggtg gaggttccca cgtacccggt gggataggtc tgggtgctgt
     8161 acagtacccc gtcccagcgg gtccaagcct cagaccacga acaataaatg ctggaagggt
     8221 taatgaattc atcggtcacg gtcggcgagg agatggtgac caggggcagc tgtgggatgt
     8281 tgcccgtggt gatgtagggt gagcccatga tcatgaaccc gcgcaggagg ttcaccatgc
     8341 acaatttgga tatctgggcg gaaccgaagg tggtctgggg ggacaggccg ttcatgatca
     8401 tgccgcagac gttgccgctg ttggaaatgc gaatgagggc gctggagtcc tcggtggacg
     8461 tatacccgtt ggtctcgtag taggcctcca ccgtgctgag agtgttccgc gccgcctggt
     8521 aggtggccgc ggaccattcc ggcggctggg accccgtgtc ggtgtcgttc agaaggaacg
     8581 gccgggacgc ggtcatggtg ggcaggagcg aaaaattgaa gtcctgaccg accagggagc
     8641 ccgaactggt gagggtggcc ccgttgccgt cgcggaactg atggtcgaac cagttgctgt
     8701 tcccggcggt ggggttgccg ttgaaaaaag aggccgcccc ccgggtgttg gtcctctttt
     8761 gcggatcccc caggctgtat cccagcgtgt tgtaacgccg acggtaatag ttcccggtcc
     8821 ccacgggcag attgccgttg gcgatccaag tggcggtggc ataatcgggg tacagctccc
     8881 cgagccaatg catgccccac caggtatcgt cggcgcgcga gatcacgcgc atgtagggat
     8941 gcgtctgcgc gtcggtgatt tcgtccaccc cctccgacgc gccgccggcc ggaacccaga
     9001 ggacccgccg gacagggata ccgctgccga acccgttgga ggcgtcgtaa ccgatctcgc
     9061 cgccgatgcc gacgtaatag aaggagtacc ccgtcatgct ggtccacagg ctcaccgagt
     9121 tcatcagcgc cccgcgcagg atctggaagt acctcgggat gtcgatgttg caggcgctgt
     9181 tccatcgatc ggcgcggttg gtgaaaccgg ggtaatcggt ggtgtcgaaa ttctcgaaat
     9241 accagttgaa ccccaggttg gtcttcacgt cggcgtacgg catgtggcgc gcgtcgccga
     9301 tgaactgata ttgctcggtg accggcggcg tctgccccac ccagacgtag gtgcgggaga
     9361 cgttggtggg ttcgttgtcg gcggtgccgg tcatggtgga gagcccgatg cgggtctcga
     9421 cggtatggcg gccgggcaca agggaactgg gcgaaatccg gatgacccac cgggcggtgt
     9481 tcttggggac cgaaccggag ttggtcgtca gatcccggtc gccttcgacg aagtaggtcg
     9541 tcgccgccag cagggacggg atgtattcca acccgtagag gcgatcggtg gtcggcaggc
     9601 cgaagtttcc gttgggcgga tgccgcaggg ggctgttcca gagggtgatg agggtctggc
     9661 cgtcggccgg atagctgacg ttgtagtcgg tggtgtcgga ggcgagctgc caggcgtagg
     9721 cgctggtgtc gctgccggcg cacttgcgga cggtgatgtt gccggcgggg atctgggtgc
     9781 tggtcagcag aacggtcacc gtggagatcg cggcgctcgt ggcgtaagag tccggcgtcc
     9841 aggcgaaata gggatagacg cgcaggttga cggcggtgtt ggcgttatag gacagctgtt
     9901 ccggatgcgt gacgacccgc gcccaaggag cgttggcgct gttgagggcg gtgtcggtgg
     9961 acttggccgc gtcggaatag ttgcgcaggg gcggcaacgg caccagttcg ccgtgcaggt
    10021 tgatgaccag cgagttgagg aagtccgccg gggaggagtt catcttttcc agcagcatcc
    10081 ggtaactgat ctccggggga tccaccccgg ccgcggtggc ggaggccacc accgcgccgt
    10141 agatcgcctc ctcctcggga taacgcgccc cgtggttgta ctgatcggcg atgctgtagg
    10201 aggcgccgtt ggtgaccgtt ccgtccacgt tgatgcgtcc ggccagggag tccggcacgt
    10261 aatagaggaa gtcccccacg ctgggcttat tgacgttccc cggataaaaa tagacccctt
    10321 ggggatccgc caggtccgcc cggttggcgg cagactcgtt gatgtagggg gtgtaatagg
    10381 gatcgcggcc gtacgccagg cgggtgatgt agtgcaggcg gagctccaaa ccggggttgc
    10441 gcgtctccag gtcctgggcg atgttgtcga acatgggttt catggtggag agcgacaccc
    10501 accatccggg cacgttctcc aagaccagca cgtagacgtc gtagacctgg gtcggcaggt
    10561 tctgattgag gatggtgcgc agcacgctga cggtctccgc cagcgcctcc ttggtgtcgg
    10621 ccttatagac ccgcacgtag acccggcggg ccaacgtctc gttgggcatc tggatgacgg
    10681 aaacccggcg cacgtatttc aagccgtcgt tgccgctgat ggggtcggcg gggtcggtga
    10741 cgtccacgcg ggtggtgagg atggtcttat acgcgctgcc gtcgtcgtaa tcgtccagca
    10801 cgccgatgtt ggaattggta tcggagatga ggccgcggag ttcctccatc atctggatgg
    10861 ccttctgcgc ggcgaaggag cgttcgttga cctcgacgtt ctggctgctg atgaaccaga
    10921 acgccttcat gctggccagc gccacgatgc tgaggatggc cagggcgatc agcacctcga
    10981 cgagggtgaa cccgcgctga ttttttggaa accggccccg gatcctcatt tttgcgccga
    11041 gaacacgtgg aacgcggatt tgttttcgcg gtattggccg acctgctggc gcaccgacga
    11101 caggatgccg gagtcgtatt ggatctccgc cacgtccacg gtgccgttca tggtcagaga
    11161 gccggtcacc accgccgtgc cggagatgat ggccgggccg ttcacggtca ggttgccgtt
    11221 cacccagagg agcccgctga agagggtgtt ggaaccgctg tcgagggtga ggttcccgtt
    11281 cacgatgaga acgccgccgc cgcgcagggg gcgcgaactg ttgaacgtgg cggcgccgtt
    11341 gatgtagacc aaagccatgg acggatagga ggcggggagg gcgttcaccg aggtcacgtt
    11401 gtagtcggcc atcagtttca ggtcgtagtc cgtgacgccg aaaaccgact gcacgctgat
    11461 ggccgtggtg gtggactgca gggccggcgt ccccgtggtc cgggtgccgg cgctccacga
    11521 cccgccggtg ccggaggtgt acatggcgcc ggcggcgttg ttgccgccgg agacgcggca
    11581 gttggcgttc acggtcccgt tggcccgact ggtgatgcgg acggcggcgg cggggaccgt
    11641 cagcgagagg cgccggatct cggtggccac ccgcgaacgg gcgacgatct cgttgggagc
    11701 cacgttgaac gcgaccgtgg agttccgaag gcgatagacg tagccgacgc tttcgatgta
    11761 ccacgccaaa ccgtcgccgg cggcaaaacc atccacccgc ttgtcggtga tgtcgtgaac
    11821 ggcgtgggga tcgatggagt tggaggaagg gtcgggctgg cggcgggcct cgtaacgcgc
    11881 ccacaaccag ttgctctccc cgatgcgata ctctttgacc agtccgatcg attcgtcgat
    11941 ggtatcgctg gtgacggagt tggtgctggt gcggggatag aacgccgcgt ccggatagct
    12001 catgccgtat ttgacgctcc ccaccggctg ggaggtctgt cgcttgaacc acgccacggc
    12061 gtcggtgagg ccggcgcggg ccacgttgtc cgcctgcacg gtggcgttgg tctgcaattt
    12121 gatgtttttc cgggcgacct tcaccagttc ggcgccgatg ggaatgaagg acaggagggc
    12181 gatcaacatg acgatgagga ccaccaccga cccgcgggaa ttttccagcg gaccgacggc
    12241 gctctttaaa aatcttcgca tccgatcggg tggcatggtc ttaagcggcc cctgttttga
    12301 gacgtgtccc gcccggcccg gctgaggacc gttgattcca ttgagtcaat tcaagtatac
    12361 gatgaacgaa ccaaaagtca agtaaagttt ttctcaaatt tgttaagaaa aaatactatt
    12421 ttttatatct ataaatcaat tcgttttgct cgtgaaaacg aaatagttaa atttattaac
    12481 tcaatgaaac ggccggggag gggtgcggct tagctgaaaa tagtaactaa tagcttctat
    12541 aatccttccc gcggcgcggc cgtcgccgta gggattcacc gcgttggcca tcctccgata
    12601 gacggcccgg tcctccagaa gccgcaaaac ccaccgccgc accaccgcgg tgtcggtgcc
    12661 gatgatcttc accgtcccgg cctggaccgc ctccgggcgt tccgtcacct tccgcaacac
    12721 cagcacgggc ttgcccaggg acggcgcctc ttcctgaaga ccgccggaat ccgtcagcac
    12781 cagatgggcg gcctgcaaaa agaggacgaa ctccggataa tccaggggcg gcaacagatg
    12841 gacgttccgc gggccggaca ggaattttcg gaccgggccg aggacgtggg ggttgggatg
    12901 cacgggatag acgaagtgaa cctccggccg gcgtcgggcg acgtcggcga cggcgcggca
    12961 gaaatccgcc aggggttttc cgaaattctc gcggcgatgc gcggtgacga ggacgaattt
    13021 ttccctctcg aaccggcgga aacggccccg cggcgaccgg aaccggccgg cgcggatgtt
    13081 ggcgagcgcc aaccggaggg cgtcgaggcc ggtgttgccg gtgacgaaaa ttcccttcgg
    13141 ggacacgttc tcccgcaaaa gattttcccg cgccgcggcg gtgggggcga aatgcagggc
    13201 gcacaccgcg tccgccagac ggcggttgat ctcctccgga taggggttga gagcgtcgaa
    13261 cgaacgcagc cccgcctcca cgtgacccac ggggatcttt tggtaatacg cggccagggt
    13321 ggcgatgagg gtggtggtgg tgtccccgtg caccaggacc atgtccggtt tcgccttttg
    13381 cagcaccggc tccaggcggc ggaggcccag cacggagacg tcggtgagcg tctgcccttc
    13441 gcgcatgatg tccaggtcga aatccgcgga gatccggaag agggagagga cctggtcgag
    13501 catctcccgg tgctgggcgg tgacggcgac gagggtcttg aacgcgcggc gtcgggcccg
    13561 cagggccagc accaccggcg ccatcttgat ggcctcgggc cgggtgccga aaacgcagag
    13621 gatcttcttc acaacgccct caggaaatcc cgcacccgga gttccatctg gtccctcgtg
    13681 cggcggtagg cgtccatctg ttccgcgtcg gtgccccgga ccggcaccgg gtcgtcgacc
    13741 tgccaatgga ggcgcttcac ggtggcgggc acgtcggggc agaggctgcc gtcgccgcag
    13801 agggtgatga tgtaatccat atccttgaaa caaatcttgt ccatggtctt ggaccactga
    13861 gaggagatgt cgatgccttt ttcacgcatc acgcggaggg cgcgcggctg aacgcacacg
    13921 gccttctttc cggcgctgta gacctccagg cggaacccgc ccaaatggcg ggcccagccc
    13981 tcggcgatct ggctccggca ggagttcccc atgcacagaa aaagtatctt tactctttta
    14041 ggcatcacgc ctcttcaacc tttcgacttt taatttcaaa cgtccccctc atactcagac
    14101 ccgccccttg aacagggtga ggaggatggt gaggatggac agcaccaggg tgaccacgta
    14161 catcaaaaga acgatctcct tctgcgtcca accaaaggcc agcagacggt gatggaaatg
    14221 gagccggtcg gcctgcatca tgccttgccc gctgcggaag cggcggaaca tggcgaaggc
    14281 cacgtccaac acgggcagcg ccaccaccag caccgggatg atcaccgcca gcacggcgct
    14341 ggtcttgagg gtgccgatga tgctgatggc gccgagcatg aaacccaaaa agagagcgcc
    14401 gccgtctccc atgaacaccc gcgccggatg gaagttatac agaaggaacc ccagagacgc
    14461 ccccagaacc gccgccgaga ggatggccgc cagtttcagc tgtttggaga aaaggacgat
    14521 cttcgtctcc ccctgaagga cgcacaccac caggaacgtc ccggcggcga tggccgccaa
    14581 ccccgccgcc agcccgtcca acccgtcgat gaggttcacg gcgttcatga aacccagcag
    14641 ccacaggagc gtgatgatct gggaaaggaa gatggggaaa tcgacgaacc cgaggccggg
    14701 cagggcgagg cccgcgatgc gaaccccata aaccatggcc acgtacgcgg cgatgatctg
    14761 caccaggagt ttgggcaagg ccggcaggga tttccggtcg tcccacatgc cgagcgccag
    14821 caccaccagg gcgccgacca ggatcccgac gaattgcgcc ttgagactga ggagacccac
    14881 cacgtcgcct ccctccatca accggtgacg gtaggc
//
LOCUS       DAOTFD010000042        14888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2768_length_14888_cov_4.086429, whole genome shotgun sequence.
ACCESSION   DAOTFD010000042 DAOTFD010000000
VERSION     DAOTFD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_14888_cov_4.086429"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="P5079_04170"
     CDS             complement(<1..959)
                     /locus_tag="P5079_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRY29216.1"
                     /translation="MNHQDILRGLIEHGKEAGFLTYEEISRKLPETNLTTEELDSLFG
                     RLEELGIDVVERGEEAGRHKPADAHEEAGLPELELDTQNSIRMYLSEMGKVPLLSREQ
                     EVSLARSIKENEKALKLLVLESPITHKEIRNWEALLEQQEMTPKELMPRGRRTASELS
                     GMRRRVKTVVKSINKSEKRVVALEEKLKQRLDPPRKQRLQEMVQKERRVIVNKIVSLN
                     LNQDKIKRLINKIKNLGLKLREYQEEIQRYEKRIRLPYQELKKIYQRALRKQISPSAF
                     RRLTGYTLTGIEATMMNIENVNLRLVKMARTLPVSVEQLLSPDT"
     gene            complement(995..2827)
                     /gene="dnaG"
                     /locus_tag="P5079_04175"
     CDS             complement(995..2827)
                     /gene="dnaG"
                     /locus_tag="P5079_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY29217.1"
                     /translation="MGVSRNGVEQVRNALNPLDIIREAVPTLKQAGNRWRGLCPFHNE
                     RTPSFYFMPEKGLWHCFGACQEGGDIFKFVMKLENLTFPEALRHLAHRAGVTLEWAAS
                     DETASRETKERERLLELLTQASVFYNEALRKSADAEPARRHLAQRKIRPETVEKFQLG
                     YAPASGFLESALRRGKPIEDLIKAGLAVRSEKSGRYHDPIHGRLVFPIFDSYGRVVGF
                     GGRVLGDESSGPKYLNSPETPVYSKGRNLYGLYQGRNGLRTRGQAVIVEGYMDVVGCH
                     QAGADYAVAPLGTAFTTDQALLLKRYVQEVILLFDPDDAGTRASWRSADVLLQSDLFV
                     RVAHVAGGKDPDELVLEKGLSALDETLRAAKDVADFWLDDISRTSPHFDSLHARVRQA
                     QEFLAFVKAVPNEMLRREWVKKVATRLSLDEGSLRRELHKISAVAAKRAAAAAPPPAA
                     RPAPKPAEERRAPHVRSLEEEILQLFCNHAEAWPRLPEVPAELFHDPRCERVFSKLLA
                     QWKERGAVELGPLLAELPPEDAAWFSALAAEEKKFDDPHETLGARLKRLELVTTERER
                     RTLEKEVLQMLEGRIPRDETKISRYQSLTRLLKGVVRAEAKSAT"
     gene            complement(2980..3180)
                     /gene="rpsU"
                     /locus_tag="P5079_04180"
     CDS             complement(2980..3180)
                     /gene="rpsU"
                     /locus_tag="P5079_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRY29218.1"
                     /translation="MVLVKVREGESIEEALRRFKRECERNGIMQEIKKREFYESPSVR
                     RKKKQAEARRKMRRRMMRHNRG"
     gene            complement(3332..3676)
                     /locus_tag="P5079_04185"
     CDS             complement(3332..3676)
                     /locus_tag="P5079_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRY29219.1"
                     /translation="MSDCIFCRIVRKEIPAEIIHEDASVVAFKDIQPQAPIHILLVPK
                     KHIGRVMDLKPDDMALVADIHKIVQALARKFTLHESGFRLVANNGSNAGQAVDHLHYH
                     LLGGRKMGWPPG"
     gene            complement(3708..4943)
                     /gene="mtaB"
                     /locus_tag="P5079_04190"
     CDS             complement(3708..4943)
                     /gene="mtaB"
                     /locus_tag="P5079_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRY29220.1"
                     /translation="MKIFLHTFGCKANQYDTELLRARMERAGGVSVEDSRHADLCLVN
                     SCSVTAAADKECRQFVRRLLRDNPRARVVVTGCYATHSPEELRSLSPKVEVYSNKEKD
                     NLPACVGFELKPEVFGLSRYPTRARAFVKIQDGCRAPCRYCIIPTVRSEAWSKPVGHV
                     LKEVETLAREGHGEIVLTGIRLGLYKGELGGKKGTLGLVGLLKELVRLPGEFRLRLSS
                     LEVTEVSDDLLKLAASTEKICRHFHVPLQSGDDKVLKDMGRWYKYSVFEERVRRIRER
                     LPDAAVTSDVLVGYPTETDAAFRNTLERVEELRLSGLHVFRYSSRPGTPAAELKPLPP
                     AVLRARAAQLLALADRLRGEFRRRFVGTTRRALAEPSGAGWTDNYLRVSLPPGSPVGR
                     LVPVRVGSEEPSGVSDENP"
     gene            complement(4955..5323)
                     /locus_tag="P5079_04195"
     CDS             complement(4955..5323)
                     /locus_tag="P5079_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29221.1"
                     /translation="MKTSMSRVVVMSVLSLLGALLVSYFWQAGTVGLLLGLFIFGVVA
                     GWNARRAGWLCGIIVGLPLALFHLTRSALPDYPSLAALFGEVDYWRLVVPASVVSTGM
                     SIMGGIVGAWLQDMKYKRGT"
     gene            complement(5320..6063)
                     /locus_tag="P5079_04200"
     CDS             complement(5320..6063)
                     /locus_tag="P5079_04200"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRY29222.1"
                     /translation="MPHFFVPPDQIRENRFFLSEGESRHLSQVLRKAPGDELQLFDGQ
                     GGLYRGVIEKVSAGRVEGRLETLSPAVKRPYALRLFQGLPKGDKFEWILEKMTELGAA
                     EIVPVHTERCVAHLSSEKAPARLERWKKIVLSAAKQSGQPQVPEVRPPMSFREALARA
                     GRNGLTLVPWESETDMGLKKVLREQRGRAESVNVFIGPEGGFTADEVSLALQGGAVSV
                     TLGPAILRTETAGLFVASALLYEWGEQTK"
     gene            complement(6191..7330)
                     /gene="dnaJ"
                     /locus_tag="P5079_04205"
     CDS             complement(6191..7330)
                     /gene="dnaJ"
                     /locus_tag="P5079_04205"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRY29223.1"
                     /translation="MPTKRDYYEVLGVSKTAGADELKAAYRKAALQHHPDRNPGNKAA
                     EEKFKEVNEAYEVLSAPEKRRLYDQFGHAGVQQGGGPGGPGGFHGFEDMAGGGFGDVF
                     SDLFGDIFGGGGGGGRGQGRRGGRGADLRYDHTVTLHEAFTGTQSSLHVARRTTCSKC
                     NGSGAKPGTSRRTCSECRGAGQVRVTRGFFTLAQTCPRCRGEGQIVESPCSECRGEGR
                     VRSTDNLTVRIPPGVEDGTALRVPGAGEAGERGTPAGDLYVVVHITPDSRFERDGENL
                     LTDRHVSIAMAALGGEVEIPTVEKSVRINIPAGTQSGTLLRVRGAGMPRLKGSGRGDV
                     IVRVVVDVPTRLNKEQKRLLAELAQSMGEEGVSTDDGLFKKVFGR"
     gene            complement(7488..9383)
                     /gene="dnaK"
                     /locus_tag="P5079_04210"
     CDS             complement(7488..9383)
                     /gene="dnaK"
                     /locus_tag="P5079_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRY29224.1"
                     /translation="MAKIIGIDLGTSNSAAAVMEGGKPVIIPSAEGTTLGGKAFPSYV
                     AFTKDGQLLVGEPARRQAVTNPEGTVFAFKRKMGQDHKYSIHGKEFTPQQLSAFLLQK
                     IKRDAEAYLGDKVEKAVITVPAYFDDNQRQATKDAGRIAGLEVVRLVNEPTAASLAYG
                     LDKAGQPHKILVFDLGGGTLDVTIMEMGTVGKEATFEVLSTSGDTQLGGTDMDNALID
                     FIAEEFRKENGIDLRKDKMAMQRLKEAAEKAKIELSNVLETDINLPFITADAAGPKHL
                     ALKLTRAKLEALIAPIINRCRHPMEQAIADAKLSPSQVTKIIMVGGPTRMPIVQKFVE
                     DFISRKSEGGVNPMECVALGAAIQAGVLGGEVKDVLLLDVTPLSLGIETLGGVMTRLI
                     ERNTTIPTRKSQTFSTAADNQPAVTVHVLQGERPMAKDNVSLGHFDLDGIPPAPRGMP
                     QIEVTFDIDANGILNVSAKDLGTQKTQHITIHAKNKLDKAEVEKFVQEAEKFADEDKK
                     KKEEIEARNEADAVVYSTEKALKDYGDKVSQEERMAIDRALSDAKEALKGSDIDRIKR
                     AKEDLLKASHKLAEEMYKSASAKAQPGSGPQPGASASGPSAEGEPGGNGKVVDAEVVD
                     RDDKKND"
     gene            complement(9420..10034)
                     /locus_tag="P5079_04215"
     CDS             complement(9420..10034)
                     /locus_tag="P5079_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRY29225.1"
                     /translation="MWGKSKEAPPGQKEKKSGTDDVGAVAADEKTSELAILKESLDQA
                     RAQSAEYYDQLVRLKAEFENFRKRTEKEKIEVSRRGREDVLIQLVGLLDVMEHAETAA
                     HKTTDLKSVVAGLDMLYKEFRRFLEKEGVSEINPKGQKFDAVEHEAVEIVEHDDDGQV
                     LDVLQKGYRFKDALLRPARVKVSKKAAAEKDAGPVDKKPEDLSV"
     gene            complement(10037..11119)
                     /gene="hrcA"
                     /locus_tag="P5079_04220"
     CDS             complement(10037..11119)
                     /gene="hrcA"
                     /locus_tag="P5079_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRY29226.1"
                     /translation="MRTLSIAEQEDRKRKLLQAIIHQYIKTGKPVGSAFLAEEDKLDL
                     SSATIRNVMAELENEGYLTHPHTSAGREPTDKAYRYYVDTLVELQHLAVMEEQRIRQE
                     YEARIREIEQVLLSTSKTLSVLSHYTGFVVPPKLEETKLRHLQLVPLDDCRVLVVLVS
                     DTGIVKQRVVMFESPCAPDTLSSMSKMLNDYLRGRSFNEVRDSILDHLAAARQRHEDM
                     FDLAHMLASQTFDMIDESSLYLEGAGNILSLPDFQDQEHMSHLIHLLDQRQNLGELLL
                     RELSGPAAEDKWKGVRVKIGSENKLADLKDLSLVSSTYAIGDKKVGILGIIGPKRMEY
                     SRMISLVGHIASTVSGALNKLVGGKS"
     gene            complement(11231..12352)
                     /gene="hemW"
                     /locus_tag="P5079_04225"
     CDS             complement(11231..12352)
                     /gene="hemW"
                     /locus_tag="P5079_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRY29227.1"
                     /translation="MTTSPGGSSALYIHLPFCRAKCRYCDFASFPDRAADIPRYLAAL
                     SLEMEAHRDKPLETLYVGGGTPTLLEPGQWRSLMSSVRRRFSLSGPCEATVECNPESV
                     TREKLAALSAEGVNRLSFGLQSDRDEFLARLGRLHDVARFREAWALARSLGFSNVNLD
                     LIFGIPGQTLADWKETLDRALEFSPEHVSAYALSVEEGTEFHRRGVKPDSDLQADMYE
                     AAADTLESAGYVHYEISNFARPGFECRHNLRYWRNLPSVGVGVSAASYENGVRRKNTA
                     SLDDYLRAVESGRSPAVEEDRLPTDQQKGEDLMLALRLKEGMSLSPEMDRLYGGVLRR
                     YQILGFLTFDPTANTVRPTRLGWRLSNQLFQELLTPSPA"
     gene            complement(12349..13296)
                     /gene="lepB"
                     /locus_tag="P5079_04230"
     CDS             complement(12349..13296)
                     /gene="lepB"
                     /locus_tag="P5079_04230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY29228.1"
                     /translation="MEEKLFWISIACGAYAFLLRWFVKKEARLQDWRLTAAMHGIFWM
                     LTGVVAGSLFVSYAERAELTWPLMAGLGAAGAVLGVLLRALRNRSSAEISWKLIREDL
                     EWADTGFSAILLASFIMYFFVQAFKIPSGSMRNTLMEGDHLFVNKFVYGVRIPYTTKK
                     MARFRQVRRGDVVVFRFPTNDKRSPHYGKDFIKRAVALPGDTVEIRRKKLFVNGEPVV
                     EPYTNFAEDYVIPPSRLFPEPGEFQKQWEIGAMAQLAGETVRDNFGPVTVPSGHYFVM
                     GDNRDRSFDARFWGPLSDKYLKGRAWVVYLPIRRAKVIR"
     gene            complement(13382..>14888)
                     /gene="lepA"
                     /locus_tag="P5079_04235"
     CDS             complement(13382..>14888)
                     /gene="lepA"
                     /locus_tag="P5079_04235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRY29229.1"
                     /translation="LVDASQGVEAQTLANALLAQEAGLRLIPVINKIDLPSADPEGVK
                     KQLREAVGLEGPFYPTSAKEGRGVLEVLQAIVEQVPPPRGNPQEPLAALVFDSVYDAF
                     RGVIVYVRILDGFLRPGMDVRFMATGASYTVEEVGHLKLKYVRAERLSAGEVGYVVCG
                     LKDIHSVKVGDTLTEEYRPTSRPHPGYREMKPFVFAGFYPMAQGDYENLKKAIEKLHL
                     QDAAFFYQAENSVALGFGFRCGFLGLLHLDIISERLRREFNVDLLVTAPTVIYRVTDQ
                     TGVQKELDSPAKFPPHGDILEIQEPYVLATVVVPSDYVGPVMQLCQDRRGNFLDMRYM
                     TPTRVVLKYEMPLSEIIIDFFDQLKSLSKGYASFDYEHIGFRPGDLERLDVLINNEIV
                     DAFSMVVPKAAAYYRGRDITERLRELIPRQMFEIPIQAAVGGRIVARETIRSMRKDVL
                     SKCYGGDITRKRKLLEKQKEGKKRMRQFGQVEIPQEAFLAVLRLGETKTEE"
BASE COUNT         2577 a         4589 c         4814 g         2908 t
ORIGIN      
        1 gtgtcggggg acaggagctg ttccacggac accggcaggg tgcgcgccat cttcaccagg
       61 cgcaggttca cgttttcgat gttcatcatg gtggcttcga tgccggtgag ggtatagccc
      121 gtgaggcggc ggaacgccga cggcgatatc tgtttgcgca gggcccgctg atagatcttc
      181 ttcagttcct ggtacggcag gcggatgcgc ttctcgtagc gctggatctc ctcctgatat
      241 tcccgcagtt tcaggccgag gttcttgatc ttgttgatga gccgcttgat cttgtcctgg
      301 ttgaggttga ggctgacgat cttattgacg atgacgcggc gttccttctg caccatctcc
      361 tggaggcgct gcttgcgcgg cgggtcgagg cgctgtttaa gtttctcctc gagggcgacg
      421 acgcgctttt cgctcttatt gatggatttc accaccgtct tcacgcggcg gcgcatgccg
      481 gacagctccg acgcggtgcg tcggccgcgc ggcatcagct ccttgggggt catctcctgc
      541 tgttcgagga gggcttccca gttgcgaatc tccttgtggg tgatgggcga ctccagcacc
      601 agaagtttca gcgccttctc gttctccttg atgctccgcg cgagggagac ctcctgttcg
      661 cgggagagca ggggcacctt gcccatttcg gagaggtaca tgcggatgga gttctgggtg
      721 tccagctcca gctcgggcag gccggcctct tcgtgggcgt cggccggttt gtggcggccg
      781 gcctcttcgc cgcgttccac cacgtcgatg ccgagctcct ccaggcggcc gaagagcgag
      841 tccagttctt cggtggtcag gttcgtttca gggagtttcc gggaaatctc ctcgtaggtg
      901 aggaacccgg cctctttgcc gtgttcgatg agccctcgca aaatatcctg gtgattcatt
      961 cgatgtccgt ccattctttc cgtttcaaac tccgctacgt cgcgcttttc gcctcggccc
     1021 gcaccacgcc cttaagcagc cgggtgagac tctgataccg tgaaatcttc gtttcgtccc
     1081 tcgggatgcg gccttccagc atctgcagga cttccttctc cagcgtccgg cgttcgcgtt
     1141 cggtggtgac gagttccagc cgtttgagcc gggcgccgag ggtctcgtgc gggtcgtcga
     1201 acttcttctc ctcggccgcc agcgcggaga accaggcggc gtcctcgggg ggcagttccg
     1261 ccagcagggg accgagctcc acagcccccc tctccttcca ctgagccaaa agtttcgaaa
     1321 acactcgttc gcagcgcgga tcgtggaaca actccgccgg cacctccggc agccggggcc
     1381 aagcctcggc gtggttgcaa aagagctgaa ggatctcctc ttccaggctg cgcacgtgcg
     1441 gggcgcgtcg ttcctccgcg ggtttcggag ccggccgggc cgccggggga ggggcggccg
     1501 ccgcggcccg tttggccgcc accgcggaga tcttgtgcag ttcccggcgc aaagagccct
     1561 cgtccaacga caggcgcgtg gcgactttct tcacccactc tcgccgaagc atctcgttcg
     1621 gcaccgcctt cacgaaagcc aaaaattcct gggcctgccg cacccgcgcg tggagactgt
     1681 cgaaatgcgg cgaggtgcgg gaaatgtcgt ccagccaaaa atcggccaca tctttggccg
     1741 cccgcagcgt ctcgtccagc gccgacaggc ctttttccag aaccagctcg tccgggtcct
     1801 tgccgccggc cacgtgcgcc acgcgcacaa aaaggtcgct ctgcaaaagc acgtcggcgc
     1861 tgcgccagga ggcccgcgtg ccggcgtcgt ccgggtcgaa caacaaaatc acttcctgca
     1921 cgtagcgttt cagcagcagc gcctggtcgg tggtgaaggc ggtgcccaaa ggggccacgg
     1981 cataatccgc gccggcctgg tggcacccca ccacgtccat gtatccttcc acgatcaccg
     2041 cctggccgcg ggtgcgcaac ccgttgcggc cctgataaag accgtagagg ttccgcccct
     2101 tggaatagac cggggtctcg ggggagttga ggtatttcgg accggacgac tcgtcgccaa
     2161 gaacgcggcc gccgaacccc accacgcggc cgtaggaatc aaagatcggg aacaccagcc
     2221 ggccgtggat cgggtcgtga taccgtccgg acttctccga acgcaccgcc aggccggcct
     2281 tgatcaaatc ctcgatgggc tttccccgcc gcagggcgga ttccaggaac cccgacgccg
     2341 gggcgtagcc caactgaaat ttttccaccg tttccggccg gatcttgcgc tgggccaggt
     2401 gacgacgggc cggctcggcg tcggccgatt tgcgcagggc ctcgttatag aaaaccgacg
     2461 cctgggtcag aagttccaga agccgttcgc gttccttggt ctcgcgcgag gcggtctcgt
     2521 cggaagcggc ccattccagc gtgacgccgg cccgatgggc caggtggcgg agcgcctcgg
     2581 ggaaggtgag gttctccagc ttcatcacga acttgaaaat gtcgccgcct tcctgacagg
     2641 cgccgaaaca gtgccaaaga cccttttcgg gcatgaaata gaacgaaggc gtccgttcgt
     2701 tgtggaacgg acacaggccg cgccagcggt tgcccgcctg tttcagggtg gggaccgctt
     2761 ctcgaatgat gtccagcggg ttgagggcat tgcggacttg ttcgacgccg ttacgggaaa
     2821 cgcccatttt tcaacaaccc cttagccgac gattcacacg cgcccgaccg gcccgcgggg
     2881 cggacgaaaa ccgttccgca acgccggcgg catcctcgaa agaaacggac gatgtctgtt
     2941 tgagagtcag tcggttgaac gcgtggagcg aggagccggt caaccccggt tgtggcgcat
     3001 catgcggcgg cgcatcttgc gccgcgcttc ggcctgtttc tttttacgtc ttaccgaagg
     3061 ggactcgtag aactcgcgtt tcttgatctc ctgcatgatg ccgttgcgct cacattctct
     3121 cttgaaccgg cgcagggcct cttcgatgga ttccccttcg cgaactttaa ccaaaaccat
     3181 gtatgaaaca ccaccttcct gaaatgggtc cggcgaggac ccctaaaaaa tatttcccgc
     3241 tggcaaaaaa agaccgccgg gccggagtac gttcgggccg cgttgcggga tgcttccgat
     3301 gagacgctgt ttaccgtcga ggatcggcga atcagccggg aggccaaccc attttgcgtc
     3361 cacccagcag atgataatgc aggtggtcta ccgcctggcc ggcgttggaa ccgttgttcg
     3421 ccacgaggcg aaaaccgctt tcgtgcaggg tgaatttccg ggccaacgcc tgcacgattt
     3481 tatggatgtc ggccaccagc gccatgtcgt cgggtttcaa gtccatcacc cggccgatgt
     3541 gtttcttcgg caccagcaaa atatgaatcg gcgcctgagg ctgaatgtcc ttgaaggcga
     3601 cgaccgaagc gtcctcgtgg atgatctccg ccgggatctc cttgcggacg atccggcaaa
     3661 agatgcaatc actcatgggg ggagtataaa cgatttaggg cgatttttca agggttttcg
     3721 tccgaaaccc cggagggctc ctccgacccc acccgcaccg gcaccaaacg gccgacgggg
     3781 gacccgggag gcagggatac gcgcaggtaa ttgtccgtcc agcccgcccc ggagggttcg
     3841 gccaaggccc gccgggtagt gcccacaaaa cgacgccgga actcccccct caaccggtcg
     3901 gccagggcca gcagttgggc ggcccgggcg cggaggacgg ccggcggaag cggtttcaat
     3961 tccgcggcgg gcgtgccggg gcgggacgag taacggaaga catgaaggcc gctgagtcgg
     4021 agttcctcca cccgctccaa cgtgtttcga aaagccgcat cggtctccgt ggggtagccc
     4081 accagcacat cggaggtgac ggccgcgtcc ggcaaccgtt cccggatgcg gcgaacgcgc
     4141 tcctcaaaaa cagagtattt ataccaccgg cccatgtctt taagaacctt gtcgtccccc
     4201 gattgaagcg gcacgtgaaa gtgccggcag attttttccg tggaggcggc cagtttcaaa
     4261 agatcgtcgc tgacttccgt cacctccaaa gacgagagcc ggaggcggaa ctcgccgggg
     4321 agccggacca actctttcag gagtccgacg aggcccaacg tccccttttt gcccccgagt
     4381 tcccccttat acaatcccaa gcggatgccc gtcagcacga tttcgccgtg cccctcgcgc
     4441 gccaaggttt ccacttcttt caaaacgtgg cccacgggtt tactccacgc ctcggaccgc
     4501 acggtgggga tgatgcaata gcggcagggc gcgcgacagc cgtcctggat tttcacgaac
     4561 gcgcgggcgc gcgtagggta acgggaaaga ccgaagacct ccggtttcag ttcgaacccc
     4621 acgcaggcgg gcaaattgtc tttctccttg ttgctgtaaa cttcgacctt cggcgacaag
     4681 gaacgaagtt cctcgggcga atgggtggcg taacagccgg tcaccaccac gcgggcgcgg
     4741 gggttatcgc gcaggagccg gcgcacgaac tggcggcatt ccttgtcggc agcggcggtg
     4801 acggagcagg agttcaccag gcacaggtcg gcgtggcggg aatcctcgac ggagaccccg
     4861 ccggcgcgtt ccatgcgggc gcgaagaagt tcggtgtcgt attggttggc cttgcagccg
     4921 aaggtgtgca ggaagatttt cacttcgtct tatttcaggt gcctcttttg tatttcatgt
     4981 cctgcagcca ggcgccgacg atgccgccca tgatggacat gccggtgctg accaccgagg
     5041 ctggcaccac cagccgccag tagtccacct cgccgaacaa ggccgcgagg gatggatagt
     5101 ccggcaaggc ggaccgggtg aggtggaaca gcgccagcgg caggcccacg atgatgccgc
     5161 agagccagcc ggcccggcga gcgttccaac cggcgacgac gccgaagatg aagagtccca
     5221 gcaacaggcc gacggtcccc gcctgccaga aataactgac cagcaacgcc cccaaaagcg
     5281 acaacaccga catcaccaca acccggctca tggacgtttt catttggttt gttcccccca
     5341 ttcataaaga agtgccgaag cgacgaagag ccccgccgtt tcggtgcgca aaatcgccgg
     5401 gcccaaggtg acggagaccg cgccgccctg gagggccaga gacacctcgt cggcggtgaa
     5461 cccgccttcc ggcccgatga acacgttgac gctctccgcc cggccccgtt gttcccgcaa
     5521 aacctttttc aaacccatgt cggtctcgga ttcccacgga acgagcgtga gcccgttccg
     5581 gccggcgcgg gccagcgcct cccggaaaga catgggcggg cggacttccg gaacctgcgg
     5641 ctggcccgac tgtttggccg ccgagaggac gatcttcttc cagcgttcca ggcgtgcggg
     5701 cgccttttcc gacgacaaat gcgccacgca gcgttccgtg tgcaccggca cgatctccgc
     5761 cgcgccgagt tccgtcatct tctccaggat ccactcgaac ttgtcccctt tgggcagtcc
     5821 ctgaaaaaga cggagcgcgt acggcctttt gacggcggga gacaacgttt ccaagcgccc
     5881 ctccacccgg ccggccgaga ctttttcgat gacgccgcgg tagaggccgc cctgcccgtc
     5941 gaacaactgg agctcgtcgc ccggcgcctt ccttaaaacc tgggacaggt gccgcgattc
     6001 cccctcggac aggaaaaatc tgttttcgcg gatttggtcg ggagggacga agaagtgggg
     6061 catgcggaaa agttagaagt gagaagtcaa aagttcaaag tttaacgggg aaagtgcaag
     6121 gcttaatgcg tgcacggcgt caaaaacccg gccctttcaa ccttaaactt aaaaccttca
     6181 actctcttcc tcaccggccg aagactttct tgaagagtcc gtcgtcggtg gagacgcctt
     6241 cctcccccat ggactgggcc agttccgcca agagccgttt ctgttccttg ttgagccgcg
     6301 tggggacatc caccacgacg cgcacgatga cgtcgccccg gccggaccct ttgaggcgcg
     6361 gcatgccggc gccgcgcaca cgcaacagcg tgcccgactg ggtgccggcg gggatgttga
     6421 tgcggacgga tttttcaacg gtggggatct ccacctcgcc gccgagcgcc gccatggcga
     6481 tggagacgtg gcgatcggtg aggaggttct cgccgtcgcg ttcgaagcgg ctgtccgggg
     6541 tgatatggac gacgacgtac aggtcgccgg ccggggtacc gcgttccccc gcctcgccgg
     6601 cgccgggcac gcgcagagcg gtgccgtcct ccacgccggg cgggatgcgc acggtgaggt
     6661 tgtcggtgct gcggacgcgg ccctcgccgc ggcattcgga acaggggctt tcgacgatct
     6721 gcccctcgcc gcggcagcgg gggcaggtct gggccagggt gaagaaaccc cgggtgacgc
     6781 gcacctggcc ggcgccgcgg cattccgaac aggtgcgtcg ggaggtgccg ggcttggcgc
     6841 cgctgccgtt gcatttggaa caggtggtgc ggcgcgccac gtgcagagag gattgggtgc
     6901 cggtgaaggc ctcgtgcagg gtgacggtgt ggtcgtaccg caggtccgcg ccgcggccgc
     6961 cgcgccgccc ctgcccgcgc cccccgccgc ctccgccgcc gaaaatgtcg ccgaagaggt
     7021 cagagaacac gtcgccgaac ccgccgccgg ccatgtcctc gaacccatgg aacccgccgg
     7081 ggccgcccgg tccgccgccc tgctggacgc cggcgtggcc gaactggtcg tagaggcggc
     7141 gcttctcggg ggcggagagc acctcgtagg cctcgttgac ctctttgaac ttctcctccg
     7201 cggccttgtt gccggggttg cggtcgggat gatgctgaag ggccgccttt cgataggcgg
     7261 ccttcagttc gtcggcaccg gcggtcttgg agacgccgag gacttcgtag taatcgcgtt
     7321 ttgtcggcat gttatgtggt gaatgaatag tgaaatggtg gagtggtgaa tgatggaatg
     7381 aaaaaacgcg acatcctatt cacctctcaa tccttttcga tccgtcattc ccccctctct
     7441 ccggaacgga gaaagaaacg cctctttccc ttcaaacctt cgacgtttca atcgttcttc
     7501 ttgtcgtcac ggtccacgac ttcggcgtcg accaccttgc cgttgccgcc gggttccccc
     7561 tcggcggacg ggccggaggc ggacgctccc ggctgcgggc cggagccggg ctgggccttg
     7621 gcggaggcgc ttttatacat ctcctcggcc agtttatggg acgccttgag caggtcttcc
     7681 ttggcacgtt tgatgcggtc gatgtcggac cctttgaggg cctctttggc gtcggagagg
     7741 gcgcggtcga tggccatgcg ctcctcctgg gacaccttgt cgccgtaatc cttcagggcc
     7801 ttttcggtgg aatagacgac ggcgtcggcc tcgttgcgcg cctcgatctc ctccttcttc
     7861 ttcttgtcct cgtcggcgaa cttctcggcc tcctggacga acttctccac ctcggccttg
     7921 tccagcttgt tcttggcgtg gatggtgatg tgctgggtct tctgggtgcc gaggtccttg
     7981 gcggacacgt tgaggatgcc gttggcgtcg atgtcgaagg tgacctcgat ctggggcatg
     8041 ccgcgcgggg ccggtgggat gccgtcgagg tcgaaatgcc ccagcgacac gttgtccttg
     8101 gccatgggcc gttcgccctg cagcacgtgc acggtgaccg cgggctggtt gtcggcggcg
     8161 gtggagaagg tctgcgactt gcgcgtgggg atggtggtgt tgcgttcgat gaggcgcgtc
     8221 atcacgccgc ccagggtctc gatgcccagg gagagcgggg tgacgtcgag caggaggacg
     8281 tccttcacct cgccgccgag cacgccggcc tggatggccg cgcccagcgc cacgcattcc
     8341 atggggttga cgccgccctc ggacttgcgg gagatgaagt cttccacgaa tttctgcacg
     8401 atgggcatgc gggtgggccc gccgaccatg atgatcttgg tgacctgcga cggcgagagc
     8461 ttggcgtcgg cgatggcctg ttccatgggg tgccggcagc ggttgatgat gggcgcgatg
     8521 agggcctcca gtttggcgcg ggtgagtttc agcgccaagt gtttggggcc ggcggcgtcg
     8581 gcggtgatga agggcaggtt gatgtcggtc tccagcacgt tggagagttc gatcttggcc
     8641 ttctcggccg cctccttgag ccgctgcatg gccatcttgt ccttgcggag gtcgatgccg
     8701 ttctcctttc ggaactcctc ggcgatgaag tcgatgaggg cgttgtccat gtcggtgccg
     8761 ccgagctggg tgtcgccgga ggtggagagc acctcgaagg tggcctcctt gccgacggta
     8821 cccatctcca tgatggtgac gtcgagcgtg ccgccgccga ggtcgaacac caggatcttg
     8881 tgcggctggc cggccttgtc gagcccgtag gcgaggctgg cggcggtggg ttcgttgacg
     8941 agccggacca cttccagtcc ggcgatgcgg ccggcgtcct tggtggcctg gcgctggttg
     9001 tcatcgaagt aggccggcac ggtgatgacg gccttctcca ccttgtcgcc caggtaggcc
     9061 tcggcgtccc gtttgatctt ctgcaggagg aacgcggaga gctgctgcgg cgtgaactcc
     9121 ttgccgtgga tgctgtattt gtggtcctgt cccatcttcc tcttgaaggc gaacaccgtg
     9181 ccttccgggt tggtgaccgc ctggcggcgg gcgggttccc ccaccagcag ctggccgtcc
     9241 ttggtgaaag ccacgtagga cgggaaggcc ttgccgccga gggtggtgcc ttcggcggac
     9301 gggatgatga ccggtttacc gccttccatg acggcggcgg cggagttgga cgttcccaaa
     9361 tcaattccga tgatctttgc catgtttgtt taccccctta aaatgatggt gatgtcggtt
     9421 cagacagaca aatcttccgg cttcttgtcg acgggcccgg cgtctttttc ggcggcggcc
     9481 tttttggaca ccttgacgcg cgccgggcgg agcaacgcgt ccttgaagcg gtatcccttc
     9541 tgcagcacat cgagcacctg tccgtcgtcg tcatgttcca cgatctccac ggcctcgtgc
     9601 tccacggcgt cgaacttttg ccccttgggg ttgatctccg aaaccccctc tttttccaaa
     9661 aaccggcgga actccttata cagcatgtcc aacccggcca ccacggactt cagatcggtg
     9721 gttttgtgcg ccgcggtctc ggcgtgttcc atcacgtcca gcaggcccac cagctggatg
     9781 agcacgtcct cccgtccgcg gcgggagacc tcgatctttt ccttttcggt ccgtttgcgg
     9841 aaattctcga actccgcctt cagccgcacc agctggtcgt agtattcggc ggactgggca
     9901 cgggcctggt cgagggactc cttcaaaatg gccagctccg aggtcttctc gtcggcggcc
     9961 acggcgccga catcgtcggt ccccgacttc ttctccttct gtcccggcgg cgcctctttc
    10021 gatttgcccc acatggtcag gatttccccc ccaccagttt gttcagggcg ccgctgacgg
    10081 tgctggcgat gtggcccacc aagctgatca tgcgggaata ctccatgcgt ttgggtccga
    10141 tgatgccgag gatgccgacc ttcttgtcgc cgatggcata ggtggacgat accaggctga
    10201 ggtccttcaa gtccgccaat ttgttctccg acccgatctt aacgcggacg cccttccact
    10261 tgtcctccgc ggccggaccg gaaagctcgc gcaggaggag ttcgccgagg ttctggcgct
    10321 ggtcgagcag gtggatgagg tgactcatgt gttcctggtc ctgaaaatcg gggagagaaa
    10381 ggatgttgcc cgcgccttcg agatagaggc tgctttcgtc gatcatgtcg aaggtctggg
    10441 acgcgagcat gtgggccagg tcgaacatgt cctcatggcg ctgacgggcg gcggcgaggt
    10501 ggtccaggat ggagtcgcgc acctcgttga aggaccggcc gcggagatag tcgttgagca
    10561 tcttcgacat ggacgagagg gtgtcggggg cgcagggcga ttcgaacatg accacgcgct
    10621 gtttgacgat gccggtgtcg gacaccagca ccaccagcac ccggcaatcg tccagcggca
    10681 cgagctgcag gtggcggagc ttggtctcct ccagcttggg cggcaccacg aacccggtgt
    10741 agtgggacag aaccgagagg gtcttggagg tggacaggag aacctgttcg atctcccgga
    10801 tacgcgcctc gtattcctgc cggatgcgtt gttcctccat cacggccagg tgttgcagtt
    10861 ccaccagcgt gtccacgtaa taacgatagg ccttgtcggt gggttcgcgt ccggccgagg
    10921 tgtggggatg ggtgagataa ccctcgtttt ccagttccgc catgacgttg cggatggtgg
    10981 cggaggagag gtcgagcttg tcttcttcag ccaaaaaagc cgaccccacc ggcttgccgg
    11041 ttttaatgta ttggtgaatg atggcctgga gcagtttccg cttacggtct tcctgctcgg
    11101 cgatggaaag ggtgcgcatg tctcttcctg ttctttatag gtaaaacaat gttagcactc
    11161 ttaattactg agtgctaata gtataacact ggcactgggg actgtcaagt gccaagaaac
    11221 acggcgtggt tcaggcgggc gacggggtca gaagttcctg gaacaactgg ttggaaagac
    11281 gccaaccgag gcgggtgggc cggacggtgt tggccgtggg gtcgaaagtc aaaaagccca
    11341 ggatctgata ccgccgcaga acgccgccgt agagccggtc catctccggg gaaagcgaca
    11401 tcccttcctt caggcgcaag gccagcatga ggtcttcccc cttttgttgg tcggtcggga
    11461 ggcgatcctc ctcaacggcc ggggaccggc cggactccac ggcgcggagg taatcgtcca
    11521 gggaggcggt gtttttgcgg cgaacgccgt tctcatagga agcggcggac acgcccacgc
    11581 ccacggaggg gaggttgcgc caatagcgca ggttgtggcg gcactcgaac cccggccggg
    11641 cgaagttgga aatctcatag tggacgtacc cggccgattc caacgtgtcg gcggcggcct
    11701 cgtacatgtc cgcctgcagg tcgctgtcgg gcttgacgcc gcggcggtgg aattccgtcc
    11761 cctcctccac ggaaagggcg taggccgaga catgttcggg agaaaactcc agcgcgcggt
    11821 cgagggtctc cttccaatcg gcgagggtct ggcccggaat gccgaagatg aggtccaggt
    11881 tcacgttgga gaaacccaag gaacgcgcca gggcccacgc ctcgcggaac cgggcgacat
    11941 cgtggagccg acccaggcgc gccaaaaact cgtcccggtc ggactgcagc ccgaagctga
    12001 ggcgattgac gccctcggcg gagagcgccg ccaatttctc ccgggtgacg ctttccggat
    12061 tgcattccac cgtggcttcg caggggccgg aaagagagaa ccgccggcgc accgacgaca
    12121 tcagcgagcg ccactggccc ggttcgagca gggtgggcgt gccgccgccg acatagaggg
    12181 tctcgagggg tttgtcccga tgggcctcca tttccagcga cagggcggcg aggtagcggg
    12241 ggatgtccgc ggcgcggtcc gggaaggagg cgaagtcgca atagcggcac ttggcgcgac
    12301 agaacggcag gtggatatac agggcggagg aaccgcccgg ggacgtcgtc aacggatcac
    12361 cttcgcccgg cggatgggaa gatagacgac ccaggcgcgg cccttcaggt atttgtccga
    12421 caggggcccc cagaagcggg cgtcgaagga gcggtcgcgg ttgtcgccca tcacaaaata
    12481 gtgccccgaa gggacggtga ccggaccgaa attgtcccgc acggtctccc ccgccagctg
    12541 cgccatggcc ccgatctccc attgtttctg aaactccccg ggttccggga agaggcggga
    12601 aggcgggatg acgtaatcct cggcgaagtt ggtgtagggt tcgaccacgg gttcgccgtt
    12661 gacgaacagt ttcttccgcc ggatctccac ggtatcgccc ggcaaggcga cggcccgttt
    12721 gatgaaatct ttcccgtagt gggggctcct cttgtcgttg gtggggaagc ggaacaccac
    12781 cacgtccccc ctccgcacct gccggaagcg cgccatcttt ttggtggtgt aggggatgcg
    12841 gacgccgtag acgaacttat tgacgaagag gtggtcgccc tccatgaggg tgttgcgcat
    12901 ggagcccgag gggatcttga aggcctgcac gaagaaatac atgatgaagg aggcgagcag
    12961 gatggcggaa aaaccggtgt cggcccattc gaggtcctcg cgaatgagtt tccaggagat
    13021 ctcggccgag gaacggttcc gcaaggcgcg caggagcacg cccagcaccg cgccggcggc
    13081 gccgagcccg gccatgagcg gccaggtcaa ttcggcgcgc tccgcgtagg aaacgaagag
    13141 ggaccccgcg accacgccgg tcagcatcca gaaaatgccg tgcatcgcgg ccgtcaaccg
    13201 ccaatcctgg agacgcgcct cttttttaac gaaccagcgc agcaagaagg cgtaggcgcc
    13261 gcaggcgatt gaaatccaaa aaagcttctc ttccatcttc ctctcgtttc ccgccggtca
    13321 ggccgaaggc cgccggacgc gttgaagtgt tcttttcgtc gcttctagaa aatcggccga
    13381 atcattcctc ggttttcgtc tcgccgaggc gcagcaccgc caggaaggcc tcctgcggga
    13441 tctccacctg gccgaactgc cgcatccgtt tcttcccctc cttctgtttt tccaggagtt
    13501 tccgttttcg ggtgatgtcg ccgccgtaac atttggacag cacgtccttg cgcatggagc
    13561 ggatggtctc gcgcgcgacg atgcgtccgc cgacggcggc ctggatgggg atctcgaaca
    13621 tctggcgcgg gatgagttcc cgcagacgtt cggtgatgtc ccgtccgcgg tagtaggcgg
    13681 cggccttggg cacgaccatg gagaaggcgt ccacgatctc gttgttgatg agcacgtcga
    13741 gccgctccag gtcgccgggg cggaacccga tgtgctcgta atcgaacgag gcgtagccct
    13801 tggaaaggga cttcagctgg tcgaagaaat cgatgatgat ctcggacaac ggcatctcgt
    13861 atttcagcac cacgcgggtg ggcgtcatgt agcgcatgtc gaggaaattc ccgcgccggt
    13921 cctggcagag ctgcatcacc gggccgacgt agtcggacgg caccacgacg gtggccagca
    13981 cgtagggttc ctggatctcc aggatgtcgc cgtgcggcgg aaacttggcg gggctgtcga
    14041 gctctttctg caccccggtc tggtcggtga cgcggtagat cacggtgggc gcggtgacga
    14101 ggaggtccac gttgaactct cgacggagcc gctcgctgat gatgtccaga tgcagcagcc
    14161 ccagaaaacc gcagcggaaa ccgaacccca gggccacgga attctccgcc tgatagaaga
    14221 acgcggcgtc ctgaagatgc agcttctcga tggccttttt caggttctca tagtccccct
    14281 gcgccatggg ataaaaaccg gcgaagacga aaggtttcat ctcgcggtat cccggatggg
    14341 gacgcgaggt gggccgatat tcctccgtga gggtgtcgcc gactttgacg gagtggatgt
    14401 ccttgaggcc gcaaaccacg tagcccacct cgccggcgga gagccgttcg gctctcacat
    14461 atttcagctt gaggtggccg acctcttcaa cggtatagga ggcgccggtc gccatgaaac
    14521 ggacgtccat gccgggccgg aggaagccgt cgaggatacg gacgtacacg atgacgccgc
    14581 ggaacgcgtc gtacaccgaa tcgaacacca gggcggccag cggctcctgt ggattcccgc
    14641 ggggcggcgg gacctgttcc acgatggcct gaaggacctc cagcacaccg cggccctcct
    14701 tggcgctggt gggatagaac ggcccttcca gtcccaccgc ctccctcaac tgttttttaa
    14761 cgccttcggg gtcggccgac ggcaggtcga tcttgttgat gaccgggatc aatcggaggc
    14821 cggcctcctg tgccagaagg gcgttggcga gcgtctgggc ctccacgccc tgggaggcgt
    14881 ccaccagg
//
LOCUS       DAOTFD010000043        14823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2781_length_14823_cov_4.556541, whole genome shotgun sequence.
ACCESSION   DAOTFD010000043 DAOTFD010000000
VERSION     DAOTFD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_14823_cov_4.556541"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="P5079_04240"
     CDS             complement(<1..1044)
                     /locus_tag="P5079_04240"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRY29230.1"
                     /translation="MGSASSAQKPKGANKDKKYRIAVMPGDGTGPEVIAEGIKILKAV
                     AASEGLSFEWIPYDLGGERYKKTGEILPGSALAELRKVDAIYLGAIGHPDVKPGILEK
                     GLLLQLRFELDQYINLRPVKLYPNVDSPLKNKGPQDIDFVVVRENTGGIYTGMGGASM
                     VGTKDEVATQLMVYTRPVVERCIRYAYEFTRKRNRKKQLTLVHKNNVLTHCGDLWVRT
                     HKEVGDKDYKDIKQDYNHVDACTMWMVKNPEFYDVIVTENLFGDIITDLGAMIQGGMG
                     IAAGGNINPEGVSMFEPIGGSAPKYTGKNVINPLAAVCAGGMMLETLGEEKAGKRVED
                     AVFKLLSSGKMKSL"
     gene            complement(1133..1519)
                     /locus_tag="P5079_04245"
     CDS             complement(1133..1519)
                     /locus_tag="P5079_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY29231.1"
                     /translation="MTESSGPEKRGAPRAATDFVVELYELDNRTLIGIGRLQNLSVSG
                     ACVESVSVLAEKTGVVVRLLLGKRHLLTLPADVVWVRNLPNTRQYGIRFGGYPEGARQ
                     LVVKFVEEYFSELKGTDFLANPPGNY"
     gene            complement(1516..2136)
                     /locus_tag="P5079_04250"
     CDS             complement(1516..2136)
                     /locus_tag="P5079_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRY29232.1"
                     /translation="MTLDTRIAKIQGRAVAVPGNEIDTDRIMPARYLKCVTFEKLGEV
                     VFQDERFDEKGAPKKHPLNDPRHRGANILVVNKNFGCGSSREHAPQGILRSGIKAVLG
                     ESFAEIFAGNCAVIGLPVATAGEQDVRRLMDAVEKDPSCEVTLDIEGKKAVCGELSFP
                     VSMPESARKSFLEGTWDSTAILMSARDEVRRTAERLPYLKGTVKPR"
     gene            complement(2133..3533)
                     /gene="leuC"
                     /locus_tag="P5079_04255"
     CDS             complement(2133..3533)
                     /gene="leuC"
                     /locus_tag="P5079_04255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRY29233.1"
                     /translation="MGKTLFDKVWDLHTVRVLPNGQTQLFIGLHLIHEVTSPQAFQML
                     RERGLKVAYPQRTFATVDHIVPTDSQTRPFPDPLAEEMIRALEKNTREFGVKFFDIAG
                     GWQGIVHVVGPEQGLTQPGMTVACGDSHTATHGAFGAIAFGVGTTQIRDILSTQTMAL
                     SRPKVRRIRVTGKLSKGVYAKDVILAVIRQLGVNGGIGFAYEFAGEVIDRMTMEERLT
                     VCNMAIEGGARVGYINPDKTTVEFLRGRPYVPKGAEFDELSAWWLSMASDKDAAYGDE
                     RTMDGQGIEPSVAWGINPGQTVGVSEKLPLPSDVPADRRSALEEAYRFTSFEPGRPVK
                     GLPIDVAFVGSCTNGRLSDLREAAKVAKGHKVAKGVRAIVVPGSQNVLREAEKEGLHE
                     IFRAAGFEWRQAGCSMCLAMNADQLKGRETSASSSNRNFIGRQGSPTGRTLLMSPAMV
                     AAAAIEGRVADCREYL"
     gene            complement(3542..5092)
                     /locus_tag="P5079_04260"
     CDS             complement(3542..5092)
                     /locus_tag="P5079_04260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRY29234.1"
                     /translation="MDAKKVFVFDTTLRDGEQSPGAALNMDEKLQAARQLAALGVDVI
                     EAGFPVSSPGDFEAVQRVAREVRGPQVCGLARAVKGDIDAAWNAVRPAKRPRIHTFIA
                     TSDLHIERKLKMTRPQVLARAVEAVRYAKKYTDNVEFSPEDAVRSDFGYLCSVVQAVI
                     EAGATTVNIPDTVGYAIPAEFGELIRRLIQRVPNIHRAIVSVHCHNDLGLAVSNSLAA
                     VKAGARQVECTINGIGERAGNASLEEVVMILKTRRDFFGLETGINAREIARTSRLVSK
                     LTGIAVQPNKAVVGANAFSHSSGIHQDGVIKERTTYEIMDPAAVGIEESTLLLTARSG
                     RNGVSNRFKHLGYRVTGPKLDDLMEKFKALADKKKYVFDDDLIALLEEDKSSRPEAFT
                     LDYLNTSSGTGVVPTATVRLRKKDEVLQEAACGDGPVDAAYRAMDKIVGLRPKLLDYT
                     LHAISSGKDAQGEVTVKVEKDGLVVSGKGTSTDIIEASAKAYLNALNKLSVPGRGKPS
                     AKNIKVRL"
     gene            complement(5113..5991)
                     /gene="pssA"
                     /locus_tag="P5079_04265"
     CDS             complement(5113..5991)
                     /gene="pssA"
                     /locus_tag="P5079_04265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRY29235.1"
                     /translation="MKPPRIVPSLFETQPNMPELPPPSTAPKPRLRQGIYMLPTLFTV
                     GNMALGFFSLIKTMAHEFSAAATAVIVAHVLDILDGRVARLTKTESKFGIELDSLADW
                     ISFGIAPAFMMYELVLKQNRLWGFPVALLFVICGGLRLARFNIKAHMGEAKTPYFVGL
                     PIPAAGGMLAIFTLLYDILEVGKPVRTLKVVMKQVPAFYDFVPAIMFVLALLMVSQVR
                     YSSLKQINLLRPRTMRALVFTLVAILMIYVYPQNTIFILYVSYIGWGMIDYFLRHVRK
                     ERPENAPGYQHDTYGK"
     gene            complement(5988..6698)
                     /locus_tag="P5079_04270"
     CDS             complement(5988..6698)
                     /locus_tag="P5079_04270"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRY29236.1"
                     /translation="MRKFRLSPVAYDGWKFIIVGLFLGGLFLFLGAWFAKVVGVLCLL
                     FAGFCVMFFRDADRQIPDTDGILSPGDGTVMEISTVDGEGYGRGKVVRIFLSIFDGHI
                     QRAPVSGTVTALRYMPGIFLDARDPRASFANESNAVDFETSWGKITVKQIAGLIARRI
                     VCWVRRDDFVNAGDRIGLIRFGSQVDLYVPMETEILVKEGDKVLAGLSIIGRHPLAAK
                     LAGSDASSAPDAQKGEKK"
     gene            complement(6804..7820)
                     /gene="ilvC"
                     /locus_tag="P5079_04275"
     CDS             complement(6804..7820)
                     /gene="ilvC"
                     /locus_tag="P5079_04275"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRY29237.1"
                     /translation="MKTKIYHDKDADLKMLKGKRVLILGYGSQGHAQALNLRDSGVNV
                     SVTELQGSRGYDLAVKHGWKPGVNLLTNNVEAVKKADWVHFLLPDELQARIWRSDVQP
                     NIKKGVVLSWSHGFNIRYGQIVPPKEADVVMIAPKGPGHLVRRTYEEGKGTPALVAVE
                     QNYSGKAKQMALAYCKAIGATRAGVLETTFTEETETDLFGEQNVLCGGTVDLIKMGFE
                     TLVEAGYQPEIAYFECLHELKLITDLIQEGGIGWMNYSISDTAEYGEYSRGPRLINEQ
                     TREIMRKNLKEVQDGSFAKEYILEKSVGAPVFNSARKAMARHPIEQVGAELRSMMPWL
                     KKKE"
     gene            complement(7855..8418)
                     /gene="ilvN"
                     /locus_tag="P5079_04280"
     CDS             complement(7855..8418)
                     /gene="ilvN"
                     /locus_tag="P5079_04280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRY29238.1"
                     /translation="MKKLPTKTSDGATDIVKTNGKKDRHTLSCLVENKAGVLARVSGL
                     FSARGYNIDSLAVGETEIPDVSRMTIVVKGDEKILEQVLKQLDKLVDVIKVSDYRATP
                     HIERDLALIKVKANKSNRSELMQICDIFRTKIIDVAADAVVIEATGDEEKIQAFLEML
                     EPFGVMEMSRTGIVAIARGKNVLSLKK"
     gene            complement(8467..10194)
                     /gene="ilvB"
                     /locus_tag="P5079_04285"
     CDS             complement(8467..10194)
                     /gene="ilvB"
                     /locus_tag="P5079_04285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRY29239.1"
                     /translation="MELTGAQIVVGSLVREGVKVAFGIPGGQALPLYDALYDAPFRLV
                     LTRHEQGASHMADGYARASGKVGVCFATSGPGATNLITGLANAYMDSIPVVAITGQVR
                     TGVIGSDAFQEADATGCTRSVTKHNFLVKDVRDLARIIREAFHIAATGRPGPVHIDVP
                     VDVQRAKTPFVWPATVRIRSYRPISAPSDDDVRRAAELIRRAQRPLLYVGGGVISSGA
                     SDALRRVAEKTDTPVANTLMANGAIPFDHPLALGPLGMHGKYVSNMALQHCDLLISCG
                     ARFDDRVTGDLKKFSPKSKKIHFDIDPACINKNVPVDVGVLGDVRRSLEKLLPLVSRK
                     SHPVWRREIKAWDKKHPLSYRLGDGLVKPQHVIKTLHRLTRGEALVVTGVGQHQMWSM
                     QWYQAKRPRHFLSSGGLGTMGFGFPAAIGAKLARPDRTVVSIEGDGSFQMTMTEMAIA
                     VREHIPIVVILLNNYYLGMVRQWQQLFYNKRFSSTALTVKGANAADGKEPTPGRDPYV
                     PDFMKWAAAYGIEGYRLTRNEQVEGALRKALAAKSPVLVEAIIPRDENVFPMIPAGMG
                     VENIIVDMA"
     gene            complement(10598..12211)
                     /locus_tag="P5079_04290"
     CDS             complement(10598..12211)
                     /locus_tag="P5079_04290"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY29240.1"
                     /translation="MKNQRTKKSKDPETGGQFSAAGGPPRIGVFVVAGAPSADETLTR
                     NLTLSVEKGIRLAGAECARFLFSGPGLVHGRDGHSCRFPRRDLWADEVECAAMSAGLD
                     GLVLCADGNDAAAGLLLGAVRLNKPALFVPLQGSQVFPDPRAALAADALSATAKQLAR
                     SRTVPTGFVWPHVPADPALDDYPSHAVACLSEVLGASLPLSSTVPTGSGEQMNLAASA
                     GQRAVDLVRQNLSIRRLLMVNAFLNAARLDAAFGGFSEALVFLLALAHEAGVKLSLEV
                     LADVERKTPQICRLHGEASHHLKDLHGAGGIPAVLAAFKGLWLPHSTVSGRGINELAK
                     SVRVRETRVIRSGAPYRAEGGVVVLKGNLAPQGALFRVPPEFPSKMGRFLGTVKVFDS
                     REDALKALLEKKIVKGDLVVVRYEGPRGGPGLRPLGLLAHVLAAQGLTDTVGLLTDGA
                     WGNALPSGLAVEFSSPEATEGAALSVLRDGDRVDVDLSTRRLNVHLTDTEMKVRLARW
                     HVPPGRARNGFLARYVRSVGPVLEGAVLK"
     gene            complement(12258..12650)
                     /locus_tag="P5079_04295"
     CDS             complement(12258..12650)
                     /locus_tag="P5079_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29241.1"
                     /translation="MDSESLNEKPPEPPAEEKTPRGMSFTKTEAELFILLLIVLGVGV
                     IAVLKGYLEHRSRPVPAHPQMTVSEEAFPPEKPVAEVPVKPKKAEEHRVVIDGEDLEL
                     PSRDVRLEPAPEKKKPKEPAEEHEITFE"
     gene            complement(12697..14280)
                     /gene="cimA"
                     /locus_tag="P5079_04300"
     CDS             complement(12697..14280)
                     /gene="cimA"
                     /locus_tag="P5079_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRY29242.1"
                     /translation="MTDRKKIQLYDTTLRDGAQGEGVSLTVDDKLKIAVVLDRIGVRY
                     IEGGFPGSNPKDEAFFRRARSLRLRQARLVAFGSTRRRDRTARRDENLAALVRVKTPT
                     VCVFGKSWDFQVVHALRASLEENLRMISDSVKFLRSRGREVIYDAEHFFDGYLARRDY
                     ALATLRAAWEAGAANLSLCDTNGGTLPFQVRDIVRAVRAALPRCPLGIHAHNDSDAAV
                     ANSLEAVREGCVLVQGTFNGLGERCGNANLVSIIPALKLKMGFDCVSDAQLLTLTESS
                     RYVSEVANLVPDDHQPYVGHSAFAHKGGVHASAMARHTRTYEHLDPSLVGNKRRILVS
                     ELSGKANVHLKAKELKLDFEKDPKAVERVIAEVKNLENQGYQFEGAEGSFSLLVSKVL
                     KSHRPYFDLKAFRVIVEKDRKTGRLVSEATLKVAVDGREEHTVGEGNGPVDALDRALR
                     RALENFYPNLRQMSLRDFKVRVINATAGTAAKVRVLVVSRDQKDEWGTIGVSENIIEA
                     SWQALVDAVEYKLMKDGRG"
     gene            complement(14325..>14823)
                     /locus_tag="P5079_04305"
     CDS             complement(14325..>14823)
                     /locus_tag="P5079_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRY29243.1"
                     /translation="VVSGVAFDRNQAKISMMDVPDRPGVAARLFGALAKVGVNVDMII
                     QSAPRDGKNDISFTISRTDMKKAMPILEQARETLRAREVACDDKVVKVSIVGVGMRSH
                     PGVAARLFGALADNGINIDMISTSEIKVACVVKEIDGEKAVRTAHKAFELDKPLPPQK
                     KRKKG"
BASE COUNT         2675 a         4769 c         4514 g         2865 t
ORIGIN      
        1 caggctcttc attttgccgc tggacaacaa cttgaaaacg gcgtcttcca cccgtttgcc
       61 ggctttttcc tcgccgagcg tttccagcat catgccgccg gcgcagacgg cggccaacgg
      121 attgatgacg ttcttccccg tgtatttggg ggccgagccg ccgatgggtt cgaacatgga
      181 aaccccttcc ggattgatgt tccctccggc ggcgatgccc atgccgccct gaatcatggc
      241 gcccaggtcg gtgatgatgt ctccgaaaag gttctccgtc acgatgacgt cgtagaattc
      301 ggggtttttc accatccaca tggtgcaagc gtccacgtgg ttatagtcct gtttgatgtc
      361 cttgtaatcc ttgtccccca cttccttgtg ggtgcggacc cacaggtcgc cgcaatgggt
      421 cagcacgttg ttcttgtgca ccagggtcag ctgttttttc ctgttgcgct tgcgggtgaa
      481 ttcgtaggca tagcggatgc accgctccac cacgggccgc gtatagacca tcaattgggt
      541 ggccacctcg tctttggtgc ccaccatgga agcgccgccc atgccggtgt aaatcccgcc
      601 ggtgttctcc cgcacgacga cgaagtcgat gtcctggggc cccttgttct tgagcggact
      661 atccacgttg ggataaagtt tcacgggccg caggttgatg tattggtcca gctcgaaccg
      721 cagctgcagc aggaggcctt tttccaggat gccgggtttc acgtcgggat gaccgatggc
      781 gccgaggtag atggcgtcca ccttgcgcag ttccgccagc gcggacccgg gcagtatctc
      841 gcccgttttt ttatagcgtt ccccgccgag gtcgtagggg atccattcga acgacaaccc
      901 ctccgaagcc gccaccgcct tgaggatctt gatgccctcg gcgatgacct ccggacccgt
      961 gccgtcgccg ggcatgacgg cgatcctgta ttttttgtcc ttgtttgcgc ctttcggttt
     1021 ctgcgcggat gaggccgaac ccatgtgact cctcctcaga gaattttgaa ttccgggatg
     1081 acgcgggacg cgccggagat cgcacggcgc gccgaccaca ctgtcttaaa aatcaataat
     1141 tgccgggagg gttggcgagg aaatccgtcc ccttcaactc gctgaaatac tcttccacga
     1201 acttgacgac gagttgacgg gcgccttccg gatatccccc gaaccggatg ccgtattgcc
     1261 gcgtgttggg caggttgcgc acccagacca cgtccgcggg cagggtcagg aggtggcgct
     1321 tgcccagcag cagacggacc accacgcccg tcttttcggc gagcacgctg accgactcca
     1381 cgcaggcgcc ggagaccgac aggttctgca gccggccgat gccgatgagg gtgcggttgt
     1441 ccaattcata gagctccacc acgaaatccg tggccgcgcg gggagcgccg cgtttttcag
     1501 gaccggaact ttccgtcatc ggggcttcac cgtccctttc agatagggga gtcgttcggc
     1561 ggtccggcgc acctcgtccc gcgcggacat caggatggcc gtggaatccc acgtgccctc
     1621 caaaaaggac ttccgcgccg attcaggcat cgagaccgga aacgacagct ccccgcacac
     1681 cgcctttttc ccctcgatat ccagcgtcac ctcgcaggac gggtccttct cgacggcgtc
     1741 catgagccgg cggacgtcct gttcgccggc ggtggccacc ggcaggccga tgacggcgca
     1801 gttcccggca aagatctcgg cgaacgactc gccgaggacg gccttgatgc cggaacggag
     1861 gatcccctgc ggggcgtgtt cccgcgaaga cccgcagccg aaatttttgt tcaccaccag
     1921 gatgttggcc ccgcgatggc gcgggtcgtt caggggatgt ttttttgggg cccctttttc
     1981 gtcgaaccgc tcgtcctgga agaccacctc ccccagcttc tcgaaggtga cgcatttcag
     2041 ataacgggcc ggcatgatgc ggtcggtgtc gatctcgttc cccggcacgg ccacggcgcg
     2101 cccctggatc ttggcgatgc gtgtgtcgag cgtcatagat actcccggca gtcggccacg
     2161 cggccctcga tggcggccgc ggccaccatg gccgggctca tcaggagggt ccgtcccgtg
     2221 ggactgcctt gtcgtccgat gaagttccgg ttggaactgg acgcgctggt ttcgcgccct
     2281 ttgagctggt cggcgttcat ggccaggcac atggaacagc ccgcctggcg ccactcgaac
     2341 ccggccgccc ggaaaatctc atgcagcccc tctttttccg cctcgcgcaa aacgttctgc
     2401 gaacccggaa ccacgatggc ccgcacccct ttggccacct tgtgtccctt ggccaccttg
     2461 gcggcctcgc gcaggtcgga cagacggccg ttggtgcagg agccgacgaa cgccacgtcg
     2521 atgggcaatc ctttgacggg gcggccgggc tcgaacgagg tgaacctata ggcctcctcc
     2581 agcgccgaac gccggtccgc cgggacgtcc gacggcaggg gcaatttttc gctgaccccg
     2641 acggtctggc cggggttgat gccccacgcc acggacggct cgatgccttg gccgtccatg
     2701 gtcctctcgt cgccgtaggc ggcgtcctta tccgacgcca tggacagcca ccacgccgaa
     2761 agctcgtcga actccgcgcc tttcggcaca tagggccggc cgcggaggaa ctcgacggtg
     2821 gtcttgtccg gattgatgta acccacccgg gcgccgcctt cgatggccat gttgcagacg
     2881 gtgaggcgtt cttccatggt catgcggtcg atgacctctc cggcgaactc ataggcgaat
     2941 ccgatgccgc cgttcacgcc cagttggcgg atgacggcca ggatgacgtc cttggcgtag
     3001 acgcccttgg acagtttacc ggtgacgcgg atcctcctca ctttgggccg ggaaagggcc
     3061 atggtctgcg tggagaggat gtcgcggatc tgcgtggtgc cgacgccgaa agcgatggcc
     3121 ccgaaagccc cgtgcgtggc ggtgtgggaa tcgccgcaag ccacggtcat ccccggctgc
     3181 gtgagtccct gttccggacc gaccacgtgc acgatgccct gccacccgcc ggcgatgtcg
     3241 aagaatttaa cgccgaactc ccgggtgttc ttctccagag cgcggatcat ctcctccgcc
     3301 aacggatctg ggaacggccg cgtctgcgag tccgtcggaa cgatgtggtc cacggtggcg
     3361 aaggtgcgtt ggggataggc caccttcagg ccgcgctcgc gcagcatctg aaaggcctgc
     3421 gggctggtga cctcgtggat gaggtgcagt ccgatgaaaa gctgggtctg tccgttcgga
     3481 aggacgcgga cggtgtgcag atcccagact ttgtcgaaga gagtctttcc catgggcgtc
     3541 ctcagagccg gaccttgatg ttcttggcgg acggcttccc gcgccccggg acggaaagct
     3601 tgttcagcgc gttcagatag gccttggcgc tggcctcgat gatatcggtg gaggtcccct
     3661 tgccggagac gacgagcccg tccttttcca ccttcaccgt cacctctccc tgggcgtcct
     3721 tgccggagga gatggcgtgg agggtgtaat ccagcagttt cggccgcaga ccgacgatct
     3781 tgtccatggc gcgataggcc gcgtccacgg ggccgtcgcc gcaggccgcc tcctgaagga
     3841 cttcgtcctt tttgcggagg cgcacggtgg cggtgggcac cacgcccgtg ccggacgagg
     3901 tgttcaaata gtccaaggtg aacgcctccg gccgggaaga tttgtcctcc tccagcaggg
     3961 cgatgaggtc gtcgtcgaac acatattttt tcttgtccgc cagcgccttg aacttttcca
     4021 tgaggtcgtc gagcttgggg cccgtcaccc ggtatcccag gtgtttgaag cggttgctca
     4081 cgccgttgcg gccggaccgc gccgtgagga gaagggtgct ctcctcgatg ccgaccgcgg
     4141 ccgggtccat gatctcatag gtcgtgcgct ccttgatcac tccgtcctga tggatgccgg
     4201 aagaatggga gaaggcgttg gcgcccacca cggccttgtt gggctgcacg gcgatgccgg
     4261 tgagtttgga caccagccgg ctggtgcggg cgatctcgcg cgcgttgatg ccggtctcca
     4321 gaccgaagaa atcgcggcgc gtcttcagga tcatcacgac ctcttccagg gaagcgttgc
     4381 cggcgcgttc gccgatgccg ttgatggtgc attccacctg ccgggccccg gccttgacgg
     4441 cggccaagga attggacacg gccaggccca ggtcgttatg acagtgcacg gaaacgatcg
     4501 cccggtggat gttgggcacg cgctggatga gccgtcggat gagctcgccg aactccgccg
     4561 ggatggcgta gccgacggta tccgggatat tgaccgtggt ggcgccggcc tcgatgacgg
     4621 cctgcaccac ggaacagagg tacccgaaat cggaacgcac cgcgtcttcc ggcgagaact
     4681 ccacgttgtc ggtgtatttc ttggcgtaac gcaccgcctc aacggcgcgg gccagcacct
     4741 gcggacgggt catcttcagt ttgcgttcga tgtgcagatc ggaggtggcg atgaaggtgt
     4801 ggatgcgcgg ccgtttggcg ggcctcaccg cgttccaggc ggcgtcgatg tcgcccttga
     4861 cggcgcgggc cagcccgcag acctgcgggc ccctcacctc gcgcgccacg cgttggaccg
     4921 cctcgaaatc gcccgggctg gagacgggga accccgcctc gatcacgtcc acgcccagcg
     4981 ccgccaactg gcgggcggcc tggagttttt catccatatt gagcgccgcg ccgggagact
     5041 gttccccgtc gcgcagcgtg gtgtcgaata cgaaaacctt tttagcgtcc atgtcctctt
     5101 ctcctcgaaa atctatttcc cgtaggtgtc gtgctgatac cccggcgcat tctccggccg
     5161 ctccttgcgg acgtgccgca ggaagtaatc gatcatcccc cagccgatgt aggagacata
     5221 aaggatgaag atggtgttct gtggatagac gtagatcatc aggatcgcca ccagggtgaa
     5281 caccagggcg cgcatggtgc gcggccggag caggttgatc tgtttcaggg acgagtaccg
     5341 cacctgagac accatcagca gcgccaacac gaacataatg gccggcacga aatcatagaa
     5401 cgccggcacc tgtttcatca ccaccttcaa cgtgcgcacc ggtttgccca cctcgaggat
     5461 gtcgtagagc agggtgaaga tggccagcat gccgccggcc gccgggatgg gcaggccgac
     5521 gaagtagggg gtcttggctt cgcccatatg ggccttgatg ttaaaccggg ccagccgcag
     5581 tccgccgcag atgacgaaga ggagggcgac ggggaaaccc cacaggcggt tctgcttcaa
     5641 caccagctca tacatcatga aggccggcgc gatgccgaag gagatccagt cggccagcga
     5701 atcgagctcg atgccgaact tgctctcggt cttggtgagg cgcgccacgc ggccgtcgag
     5761 gatgtcgagc acatgggcga cgatgaccgc ggtggcggcg gcgctgaact cgtgcgccat
     5821 ggtcttgatc agcgaaaaga aacccagcgc catgttcccg acggtgaaaa gggtggggag
     5881 catgtaaatg ccctggcgca ggcgcggctt gggggccgtg gacggaggcg gcaactccgg
     5941 catgttgggc tgcgtttcga acaaagacgg cacaatgcgg ggcggtttca ttttttttct
     6001 cccttttgcg catccggcgc ggaggacgcg tccgaacccg ccaacttggc cgccagcgga
     6061 tggcggccga tgatggacag tccggccaag actttatccc cctctttgac cagaatctcc
     6121 gtttccatgg gcacgtacag gtccacctgg gaaccgaacc gaatgagccc gatgcggtcc
     6181 ccggcgttga cgaaatcgtc ccgtctcacc caacagacga tgcgccgcgc gatgagcccg
     6241 gcgatctgtt tcaccgtgat ctttccccag gaggtctcga agtcgacggc gttgctctcg
     6301 ttggcgaacg acgcgcgcgg gtcgcgggcg tccaggaaga tgcccggcat gtaccgcaac
     6361 gccgtcaccg tgcccgagac gggcgcccgc tgaatgtgcc cgtcgaagat ggacaggaat
     6421 atccggacca ccttgccgcg gccgtatccc tcgccgtcca ccgtggagat ctccatcacg
     6481 gtgccgtccc cgggcgacag gatgccgtcg gtgtccggga tctgccggtc ggcgtcgcgg
     6541 aaaaacatca cgcaaaaccc ggcgaacaaa agacacagga cccccaccac cttggcgaac
     6601 cacgccccca ggaacaggaa aaggcccccc agaaagaggc cgacgatgat gaatttccag
     6661 ccgtcgtagg cgacgggaga gagacgaaat ttcctcatag atacctcgtt aaaagtttaa
     6721 agttgaaagc gtaaagtaag ggaaaaaaca gggtgccccc tatcctcaac ttttaacttt
     6781 tcactccaaa ccttaaaccg cctctattct ttctttttaa gccacggcat catggagcgc
     6841 aattccgccc ccacttgttc gatgggatga cgggccatgg ctttgcgggc cgagttgaac
     6901 accggcgctc cgacggactt ctccaagatg tattccttgg cgaaggaacc gtcctggact
     6961 tctttcaggt tcttccgcat gatctcgcgg gtctgctcgt tgatgaggcg cggcccgcgg
     7021 ctgtattcgc cgtattcggc ggtgtccgag atggaataat tcatccagcc gatgccgccc
     7081 tcctggatca ggtcggtgat gagcttgagc tcatggagac actcgaaata ggcgatctcc
     7141 ggctgatagc ccgcctccac gagggtctcg aaccccattt tgatgagatc cacggtcccg
     7201 ccgcaaagga cgttctgttc gccgaagagg tcggtttcgg tctcctcggt gaaggtggtc
     7261 tccaggacgc cggcccgggt ggcgccgatg gccttgcagt aggccagcgc catctgtttc
     7321 gccttgccgg aatagttctg ttccaccgcc accaacgccg gagtgccctt gccttcttca
     7381 taggtccggc gcaccagatg gccgggcccc ttgggggcga tcatcaccac atcggcctct
     7441 ttgggcggaa cgatctgccc gtaacggatg ttgaaaccgt gggaccagga gagcaccacg
     7501 ccctttttga tgttcggctg cacgtccgac cgccaaatcc gggcttgaag ttcgtccggc
     7561 agaaggaaat ggacccaatc cgctttctta acggcttcca cgttgttggt caacagattg
     7621 accccgggtt tccagccgtg tttcaccgcc agatcgtagc cccgactgcc ctgcagttcg
     7681 gtgaccgaca cgttgacgcc ggaatccctg agattcaacg cctgggcgtg gccttgggac
     7741 ccgtagccca aaatcaggac tctctttccc ttgagcatct tcagatcggc gtctttgtcg
     7801 tgatagattt tcgtcttcat cgatttgtta ccggttttca tgtgtgttgc ctccctattt
     7861 tttcaacgac agaacgtttt tgccccgggc gatggccacg atgccggtgc ggctcatctc
     7921 catgacgccg aagggctcca acatttccaa aaaagcctga atcttctcct cgtcccccgt
     7981 ggcctcgatc acgacggcgt cggcggcgac gtcgatgatc ttggtgcgga agatgtcgca
     8041 aatctgcatc agctcggacc ggttggattt attggccttg accttgatga gggccaggtc
     8101 gcgctcgatg tggggcgtgg cgcgatagtc ggagaccttg atgacgtcca ccagcttatc
     8161 gagctgtttc agcacctgtt cgaggatctt ctcgtcgccc ttgaccacga tggtcatgcg
     8221 ggacacgtcc gggatctccg tctccccgac ggccaaagaa tcgatgttgt agccccgggc
     8281 agaaaaaagc cccgacaccc gagccaacac gccggccttg ttctccacca ggcacgacag
     8341 cgtgtggcgg tctttcttgc cgttggtttt gacgatgtcg gtcgcgccgt cggacgtttt
     8401 tgtcggaagt ttcttcatga tttcaatcct catgtcctat tggaattatt tcgacgtcaa
     8461 agacttttac gccatatcga cgatgatgtt ctccacgccc atgcccgccg gtatcatggg
     8521 aaacacgttc tcgtcccgcg ggatgatggc ctccaccaaa acgggggact tggcggccaa
     8581 ggccttccgg agggcgccct ccacctgttc attcctggtc aagcgatacc cctcaatgcc
     8641 gtacgccgcg gcccatttca tgaagtccgg cacgtaggga tcgcgtcccg gagtgggctc
     8701 cttgccgtcg gcggcgttgg cccctttgac ggtgagggcc gtcgaggaga aacgtttgtt
     8761 gtagaaaagc tgctgccatt gccgcaccat gcccaggtag taattgttca gcaggatcac
     8821 cacgatgggg atatgttccc gcaccgcgat ggccatttcg gtcatggtca tctggaagga
     8881 gccgtccccc tcgatggaca ccaccgttcg gtcgggccgg gccagcttgg ccccgatggc
     8941 cgccggaaaa ccgaacccca tggtgcccag accgccggaa ctcaggaaat gccgggggcg
     9001 tttggcctga taccattgca tggaccacat ctgatgctgg cccacgcccg tcaccaccag
     9061 cgcctccccg cgcgtgagac ggtgaagggt cttgataacg tgctggggct tcacgagacc
     9121 gtcccccagg cgataagaca aaggatgctt cttgtcccag gccttgatct ctctccgcca
     9181 gacggggtgg ctcttacggg aaaccaacgg aagaagtttc tcgagagacc gtcgcacatc
     9241 ccccaacacg cccacgtcca ccggcacgtt cttgttgatg caggccgggt cgatgtcgaa
     9301 atggattttc ttcgacttgg gggagaattt cttcaggtcc cccgtcaccc ggtcatcgaa
     9361 acgggcgccg cagctgatca gcaggtcgca atgttggagg gccatgttgc tcacgtattt
     9421 accgtgcatg cccagaggcc ccagcgcgag gggatggtcg aaggggatgg ccccgttggc
     9481 catgagcgtg ttggccacgg gcgtgtccgt tttctcagcc acacgccgaa gggcgtcgga
     9541 agccccggac gaaatgacgc cgccccccac atagagcagc ggacgctggg cgcgacggat
     9601 gagctccgcc gcccgccgca cgtcgtcgtc cgaaggagcg ctgatgggcc ggtaggaacg
     9661 gatgcgcacg gtcgcgggcc acacgaaagg cgtcttcgcc ctttgaacgt ccacggggac
     9721 gtcgatgtgc accggaccgg gccgccccgt ggcggcgata tggaacgcct cgcggatgat
     9781 gcgcgccaag tcccgcacgt ccttcaccag gaaattatgt ttggtgacgg aacgggtgca
     9841 gcccgtggcg tccgcctcct gaaaagcgtc ggaaccgatg acgccggtcc ggacttgtcc
     9901 cgtgatggcc accaccggga tggagtccat ataggcgttg gccagcccgg tgatgaggtt
     9961 ggtggcgccg gggccggagg tggcgaaaca cacgcccacc tttccggaag cgcgcgcgta
    10021 accgtcggcc atgtgggaag cgccctgttc gtgccgggtc agcaccaggc ggaaaggagc
    10081 gtcgtagagg gcgtcgtaga gcggcagcgc ctggccgccg ggaatgccga aagcgacttt
    10141 cacgccttcc cggaccagac ttcccaccac gatctgtgca cccgttaatt ccattgtatc
    10201 tctcccttaa aaatggagaa aaatcgctcc cacctgacat ttccgcgacg gacggaatgg
    10261 gtatacgagg cttgtgtttt caagacagga gcccccattt tacattattt taagacggcc
    10321 aaaaataaaa aacccacagt ctttttggac tgtgggggct ttttggcttc cggcttttta
    10381 aggcgtttcg ctatttgacc gccgccgaaa ggcaaagccc ccgtgatcca aggaccacgg
    10441 gcaggctcac gagaaccaac aacaggcctt tcagcgaacg aggattcata acaggcttag
    10501 tttatcgcac ctcttggatt ttgtcaatgc cttttattta ttttccgtcg acaaaacgac
    10561 cccgcgggaa ggcgtgcccg ccggagggcg ggggccgcta tttcaacacc gccccttcca
    10621 aaaccggtcc cacggaacgg acgtagcggg ccaaaaaccc gtttcgggcg cggccgggcg
    10681 gaacgtgcca gcgcgccaaa cggaccttca tctccgtatc ggtgagatgc acattgagcc
    10741 gacgggtgga caggtccacg tccacccggt cgccgtcgcg cagaaccgac aacgccgcgc
    10801 cttccgtcgc ttcgggagac gagaactcca ccgccaaccc cgacggcaag gcgttccccc
    10861 aggccccgtc ggtgagaagg ccgacggtgt ccgtcaaccc ctgcgccgcc agcacgtgcg
    10921 ccagcaggcc caaaggcctc aatcccggac cgccccgggg cccttcataa cgcaccacca
    10981 ccaggtcgcc tttgacgatc tttttctcca gcaaggcctt cagggcgtcc tcgcgggaat
    11041 caaacacctt cacggtgccg aggaaccggc ccattttcga cggaaactcc ggcggcacgc
    11101 ggaacaacgc cccctgcggg gcgaggttgc ctttcaagac cacaacaccg ccctcggcgc
    11161 ggtaaggcgc gccggaacga atcacgcgcg tctcccggac tcggaccgac ttggccaatt
    11221 cgttgatgcc gcgcccgctg acggtggaat gcggcaacca cagtcccttg aacgcggcga
    11281 gaacggcggg gatgccgccg gcgccgtgca ggtctttgag gtggtgggag gcctcgccgt
    11341 gcaaccgaca tatctgcggc gtcttgcgtt ccacgtcggc aagcacctcc agcgagagtt
    11401 tcacaccggc ctcatgcgcc agggccaaca ggaagacaag cgcctcggaa aaaccgccga
    11461 acgcggcatc caggcgggcg gcgttcaaga aggcgttcac catcagaagc cggcgaatgg
    11521 acaagttctg ccgcaccaga tccaccgccc gttgacccgc cgaagccgcc aaattcatct
    11581 gttccccgga acccgtgggc accgtggagg acaacggaag ggacgctccc agcacctcgc
    11641 tcagacaggc gacggcgtgg gacggataat cgtcgagcgc cggatcggcg gggacatgcg
    11701 gccagacaaa acccgtcggc accgtgcggg agcgcgccag ttgtttggcc gttgcggaca
    11761 gcgcgtcggc cgccaacgcg gcccgagggt ccggaaaaac ctgcgagccc tgcagaggaa
    11821 cgaacagggc cggcttgttc aggcgcacgg cgccgagcaa cagacccgcg gccgcgtcgt
    11881 tcccatcggc acacagcacc aagccgtcca acccggcgga catcgcggcg cattccactt
    11941 cgtccgccca gaggtcccgc cgcggaaacc ggcaggaatg tccgtcgcgg ccgtggacaa
    12001 ggcccggccc cgaaaacagg aaccgcgcgc actcggcccc ggccagccga atcccctttt
    12061 ccacgctgag cgtcagattc cgcgtcaaag tctcgtcggc cgacggcgcg ccggcgacga
    12121 cgaacacccc gatgcgcggc ggcccgcccg ccgccgaaaa ctgcccgccc gtttcaggat
    12181 ccttcgactt tttggtgcgt tgatttttca cggcccgacg tcctctcgtt tctctaacga
    12241 tttaaaaatt gcggcgttca ttcgaacgtg atttcgtgct cttccgccgg ctctttcggt
    12301 tttttctttt ccggcgcggg ctccagccgc acgtcccggg aaggaagttc cagatcttcc
    12361 ccatcgatga cgacccggtg ttcctcggcc tttttcggtt tcaccggcac ctccgccacc
    12421 ggcttttccg gagggaaggc ctcctcggaa accgtcatct gcgggtgggc gggcaccggc
    12481 cgggaccggt gctccagata tcccttgagc accgcgatga cgccgacgcc caagacgatc
    12541 agaagcagta tgaaaagttc cgcctccgtc ttcgtaaaac tcatgccgcg cggcgttttt
    12601 tcctccgccg gcggttccgg cggtttttcg ttgaggcttt cggaatccat ccctcgaaac
    12661 cccctcgggc cccctccgga agacgggggc ggccgcctat ccccggccgt ccttcatgag
    12721 tttgtattcg acggcatcca ccaacgcctg ccaggaggcc tcaatgatgt tctcggagac
    12781 gccgatggtt ccccactcgt ccttctggtc ccgggagacc accagcacgc gcaccttcgc
    12841 cgccgtgccg gccgtggcgt tgatcacgcg caccttgaaa tcccggaggg acatctgccg
    12901 cagattggga taaaaattct ccaaggcccg ccgcagcgcg cggtccaggg cgtccaccgg
    12961 gccgttccct tccccgacgg tatgttcttc ccggccgtcc accgccacct tgagggtcgc
    13021 ctcggaaacg agacgacccg tcttgcggtc cttctccacg atgacgcgaa aggccttcag
    13081 atcgaaatac ggccggtgcg atttgaggac tttggagacc agcagggaga acgacccctc
    13141 ggccccttcg aattgatatc cctggttctc caggttcttc acctcggcga tcacccgctc
    13201 gacggctttc gggtctttct cgaaatccaa cttcaactct ttcgccttga ggtgcacgtt
    13261 cgccttgccg gacaactccg acaccaggat gcgccgcttg ttccccacca gggacgggtc
    13321 caggtgctcg taggtgcggg tatgccgcgc catggccgag gcatgcacgc cgcccttatg
    13381 cgcaaaagcg gagtggccca cgtacggctg atgatcgtcg gggaccaggt tggccacctc
    13441 cgaaacatac cgggaggatt ccgtgagggt cagcaactgc gcgtcgctca cgcagtcgaa
    13501 ccccatcttc agcttcaacg ccgggatgat ggagaccagg ttggcgttgc cgcagcgttc
    13561 gcccaagccg ttgaacgtcc cctgcaccag gacgcaccct tcccgcaccg cctcgagaga
    13621 attggccacg gcggcgtccg agtcgttgtg cgcatggatc cccaggggac accgcggcag
    13681 agcggcgcgg accgcgcgga cgatgtcccg aacctggaac ggcaaggtgc cgccgttggt
    13741 gtcgcacagg gagaggttgg cggcgccggc ttcccaggcc gcgcgcagcg tggcgagcgc
    13801 gtagtcgcgg cgggccagat agccgtcgaa gaaatgctcc gcgtcgtaga tcacttcccg
    13861 gccccgcgag cgaaggaact tcacggagtc cgagatcatc cggaggttct cctccaacga
    13921 cgcccggaga gcgtgcacca cctgaaaatc ccacgatttg ccgaagacgc agaccgtcgg
    13981 cgtcttgacc cggacgagcg ccgcgaggtt ctcgtcccgc cgggcggtgc ggtcgcgacg
    14041 gcgcgtgctg ccgaaagcca ccagccgcgc ctgccgcaga cgcagcgaac gggcccgacg
    14101 gaaaaacgcc tcgtccttgg ggttggagcc cgggaatccc ccctcgatat aacggacgcc
    14161 gatccggtcc aacaccaccg cgatcttgag cttatcgtcc accgtcagag aaacgccctc
    14221 cccctgggcc ccgtcgcgga gggtggtgtc gtagagttgg atttttttac gatcggtcat
    14281 gtcttttcct caaaaaaacg ccgagcctcg agcgcggagg cccttcatcc cttctttctc
    14341 ttcttctggg gcggcagcgg cttgtccagt tcgaacgcct tgtgcgcggt gcgcaccgct
    14401 ttttcgccgt cgatctcctt gaccacgcag gccaccttga tctccgacgt ggagatcata
    14461 tcgatattga tgccgttgtc cgccagggcg ccgaagagcc gggcggccac gcccggatgg
    14521 gaacgcatcc ccacgccgac gatggagacc ttgaccacct tgtcgtcgca ggccacctcg
    14581 cgggcccgga gagtttcccg cgcctgttcc aggatcggca tcgccttttt catgtccgtg
    14641 cgggagatgg tgaaagagat atcgttcttc ccgtcgcggg gagcggactg gatgatcatg
    14701 tccacgttca cgccgacctt cgccagggcg ccgaaaagcc gggcggccac gccgggccgg
    14761 tccggcacgt ccatcatgct gattttcgct tgattgcggt caaaagccac tcccgagacg
    14821 acg
//
LOCUS       DAOTFD010000044        14616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2842_length_14616_cov_3.837168, whole genome shotgun sequence.
ACCESSION   DAOTFD010000044 DAOTFD010000000
VERSION     DAOTFD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_14616_cov_3.837168"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1304)
                     /locus_tag="P5079_04310"
     CDS             complement(57..1304)
                     /locus_tag="P5079_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY29244.1"
                     /translation="MRNHILFNVLLTASASLLVFLPRWPFDRRFGIVAVVSAVLHLIL
                     YVFYPRLKSWVPLWMSYLNVFLLGVAVFYTGGILSPFVFVFSCIIILGSVSELRHRWI
                     LPATILTYVAVLLAQFSGRIPYRTVSPELVYGSWFATLLVMVTTVGFLIVSALYYDII
                     YRNVQKRVADEQREKEQLVQRMGHLEATSQIGAMVTKIAHDIHGPLSSISSFVEIHRQ
                     NENLDAEARKDFEIIVGEADRISKLIQRMLHFVKPGEEEKSEIRPAGLLETIVTVIQF
                     HPKSKGVRFETAFPGDGDLRVFGNKDSLQQVYFNIAKNAVEALEGQKVRRVTVALRRE
                     KGECVIEFCDNGPGISEERIQKILGGFHSTKEDGTGLGLVIAQEIIRSHGGRLSMESE
                     RGKGTVVRTVLPLMEEGKAPSAG"
     gene            complement(1380..2129)
                     /locus_tag="P5079_04315"
     CDS             complement(1380..2129)
                     /locus_tag="P5079_04315"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRY29245.1"
                     /translation="MPERRDYRRDPTTAADTPRPRYLQVLVGVFILPLLVLWRWLFRG
                     ANAKAVFLGFALVTLIGWAWSITLSAQQWWIFPRKWLIGIWLLPNLPLEEMLFYPMVM
                     LFMIGFYNVIDRARPQTLKSPWSYRLYLYSGTLVFAVLAFLYRDEKPYYLYSHFIVYN
                     GLAIVLCEAVWRYVNPRAMALITGFMFLVGLIWDYFAFKYDWWIYNPIMGIKFWGIPV
                     EDVNFYLYAPPAGLSLYLLAVKYTKKQDSKG"
     gene            complement(2145..2693)
                     /locus_tag="P5079_04320"
     CDS             complement(2145..2693)
                     /locus_tag="P5079_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29246.1"
                     /translation="MKLIVWEIWSYLQLPVIWYLFRRFAKKNVFGDMVAGAMIGAFIE
                     FSTEPLWTYHFRLTIYKDVPLSVVLGWGVMFTLVVFVSEKLYCWILKKTAIDPYDKKI
                     FLFDVLGAALVGLPLETVGLKSGVWDYNYDVLQWNWGTIPFFNMPYEALMGYCLLMLL
                     GPTFVRYWQGAFEGRHTRTDLP"
     gene            complement(2690..3079)
                     /locus_tag="P5079_04325"
     CDS             complement(2690..3079)
                     /locus_tag="P5079_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29247.1"
                     /translation="MPEHIQPLPPEGKKKEEFPTYRRGAWWPAFSLLLLPFLFLFKPF
                     YSVARKELNGRAALATILCFETFMLFAEHYSLKRGHWVYNEARIWGPKVFGVPLEEPL
                     LYYWFPGLFVIVFMLFVKHKIEGRKNS"
     gene            complement(3072..3413)
                     /locus_tag="P5079_04330"
     CDS             complement(3072..3413)
                     /locus_tag="P5079_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29248.1"
                     /translation="MPRYFLLYFFGFWVPSFVLMPAIWPFLEKSTRKAFWITFLGLCL
                     PTSLVVEYVYLWADVWNFSEAWDPLIGWTLFGAPVEEFFFWLGACGVIMLLYLAFDLL
                     DRRFFRRVPHA"
     gene            complement(3415..3987)
                     /locus_tag="P5079_04335"
     CDS             complement(3415..3987)
                     /locus_tag="P5079_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29249.1"
                     /translation="MDSKKQFEKYYTTAEAARLCHVSHSTIFRAALQKKLNAYSTPGG
                     HFRIAPADLEKFAKDNGLPYTPEHTRRKKILVVEDNAVELRLIKRLLEKDPFLEVQTT
                     QVGFKAGFLMQVFRPELVVLDIYLGETDGREIVRLVRADATLSTTKIVALTAAKDPEE
                     IAAIKKSGVDFFMAKPVNPKTFLSEIKALL"
     gene            4107..5354
                     /locus_tag="P5079_04340"
     CDS             4107..5354
                     /locus_tag="P5079_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY29250.1"
                     /translation="MRKRSSILFCLLFFLASLPRMARAAGYEFEGIGARQVGRAGAAV
                     ADVSDWTAIYWNPAGMAAPRLDGETNAGQVGFEAFGGYARTKDGNSLSRLAGPLFNEK
                     HMASSFILGAFGVRVPLESGWVLGTGVYTPLIQGADFNDTANGMTLDYFAQAGIAVVN
                     FSVARVLWNDVTLGAGVNAVYAQMRSNSRLTNSLSGTVANSDIAGDGWGPEGILGLQW
                     RMSSAWRFGAVYRTGSRIPLKGDADASMTGLPDERSTFGYDFNHPATAAVGVSFTPVP
                     KATLSFDINHTNWRSFRGDMDFETSGPLLSDIRNSYAWKNTFKFRLGGLYRLTDKTDL
                     LAGLSYDNYALDRNSIDFSTTIDVPIFRYFGGVTRRLPKGWEMSASLLAAKGRREKNG
                     VDYSIGGWQAMVETAWNFSTKHP"
     gene            5368..5733
                     /locus_tag="P5079_04345"
     CDS             5368..5733
                     /locus_tag="P5079_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29251.1"
                     /translation="MNDLIQTIEFFAVFSMAIEMGFATLFSLPPVYAFFKKNPDKKYL
                     KRLLVLAFCLLLCAALYKAVSADKGALFLERIFGQKVPVVDVLLSALILAGGSRAIHD
                     ILKRLSGATAQKLLQQGKP"
     gene            5871..6809
                     /locus_tag="P5079_04350"
     CDS             5871..6809
                     /locus_tag="P5079_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY29252.1"
                     /translation="MLLGWDACQRNHLKECLSREELPHLKKLISEGTVVAIDVLRHTD
                     TKAGWTQILTGYEPEITGVYSNNRFGPIPPGYSVFERLENFFGRDNIVTMVFSGKRIH
                     FDSTPPAKIPYKDADQLPPDDVSMYKTDTGGGALITEGGEKYWDVPAQPYHLMAKAVD
                     VWENALGLNDDVTSPLLEAIRRHKNERFFIFALFNDIDYFGHEFGENSPQYNDAIVSA
                     DACLGRIVSTLRELGLYDDTRIYITADHGFDEGKKNHNDAPYVFLATNDPRVIRRGER
                     RDITPTLLDRLGVPLEGITPPLDGRPLTKRHKPPRW"
     gene            complement(6820..8106)
                     /locus_tag="P5079_04355"
     CDS             complement(6820..8106)
                     /locus_tag="P5079_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY29253.1"
                     /translation="MKRKRSFLLAGVLFLSVAGLLADDQQTGLLAVPSVPIASRVGGD
                     TPVKPEGAGFFQQGVKEYIDRNDEAAIGSLKKALEANGGDEKAQRLLLKVYFRAIHDQ
                     FEKRNYRRAWEYVEEAKRYFPFDSELSALSDSVRKAVPPPAAPPPAPAPPKAAPRAAT
                     GPAVVRTAPASVPSPAPAPAPSSSREKAEETKGRYVSLGVILFLGVAVLLQKNRQKAL
                     ETEMKSVREKLNRLDTERAALQAKSETQSDMLRRMEATHKEELERLMRRFDRGLEQEA
                     APVPGKTEGPAGKQRGTLLEQMFLHRREQEIRDVLVDVTPPEREVEAGRISAQVRNLY
                     ETAPREALRFLRGLSKDANPTTRLLIVPALAETGDPAAVDVLFELERDESPEVQREVL
                     KHLKKLRNDGTGLPEECRRRIAEILEEEKSKADWVF"
     gene            complement(8103..9608)
                     /locus_tag="P5079_04360"
     CDS             complement(8103..9608)
                     /locus_tag="P5079_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29254.1"
                     /translation="MRRLLPLLLFCGVPVWAGVNVPFRDRPANAGLAGEWLTALAASA
                     RDAGLGNASVSFPGAASAYANPAGIAGNPMGEAVFSMSPLFSAGQFQSLAVAHPLTPK
                     QSVGVTLLHLGSGEAEKTDFLGQSQGSFDEQDFAFLLSYGAQVSERWSGGVNLKAVSQ
                     SVAEYSATGVGLDAGVRVKLSESFSCGAAVQNLVPPELRLNTATDRFPAVMRLGGTYG
                     YRIFGRHFSWTGEYDVYHGLGGRRIARWGGGLEALLLPTAPLPLTLRVGFNHREYSFG
                     WGIEKGPARFDYAASFHELAVVHHFGISLRYGVLSPLAEKKLASEWRKVHAREAELDA
                     MKDEVQKEKVRLAREAELRTLLLEAQLLFENAKYDESKRRLRRVLDERPEDPTALNLR
                     REIERREARIAAEGFCRQAKGFYDAGQYREALEMAKMALLRDREFADAQVLASLTQAQ
                     IYLEELNYEGAEKHLLKVVRISPENQEAQRLYERLRDVLELSEPAAAKGTP"
     gene            complement(9605..13339)
                     /locus_tag="P5079_04365"
     CDS             complement(9605..13339)
                     /locus_tag="P5079_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRY29255.1"
                     /translation="MERAGFTGFFAAALFLAPVGGQGASEKIFDSGNFFSGQTSDTEV
                     EAGYLTLAGLWGSASSPMPARYHAGFAFDEARGKAVVFGGHDQNDLKFDDTWVYDPSS
                     GWTEHVPAGGPAARYGHGLVWAGDKFLLFGGLDASGGYLNDTWMYDVVQDTWTFVASP
                     DAPEARAFFAMAYDADQDKVVLFGGEGVEGITVNAHTWVFDVAASSWSRWSPTPSPGV
                     RMGSCMAYDRSARRIILFGGRRNGWEFSEIYGDTWALDVSGMGWEQKSPSSPPGPRSE
                     AALLYDARHGQTVLFGGRDAVATYLGDIRFYHFGKDRWQEFDREVGAPPGRYGHGMAY
                     DTVRQRGMLFAGRRDVSSNLLRYYTFASTGTWTSPRVDSWESFPAGLPAPIRWGGLSA
                     VVEGSTVSFQLASSTDGVTFNDFSGPDGSSQTFYVASAAAQTIWAGHGDRRFLLLRSS
                     FSVEDVPARPWASQFKIVFNRAPFRPTLLSPADRSRLNDDTPVFAWNPVADQDGDTAL
                     RYQFQADDDPGFASPDISRDGLADASFSTTTLLTEGTWYWRVRGRDEAGLYGDWSSPF
                     QLLLDTHTPPSPVTQLAAAMGAANGSVVLSWIFPGDDKGRVDNGLCRVRYSVSGPILT
                     EPDWQAAAERTFNFSADPAQALQTAVSGLSDAATYYFAVKTEDEVGNLSALSSVSPVA
                     FTNAPPHVALVYPNGGAFVTQIATIVWIASDPNPAGTFSVSVSLSPDGGQGFPALLAS
                     LPMGTTSFLWDSRSVSNGTAYRVKVQAVDQGGFVSSAVSDGDFRVDNVNEPPVASFLA
                     PLNGQPVSGDFAVRWRALDPNTFNTHTFNLYFSTHTGAGFIPLVLNTSATTYVLRTRE
                     WPNKAGYRLMLEATDSGEPPLGHTVVTPEFAVHNSNPPRPFRLLRPLADDDPAVFDVS
                     FAWEAGVDPDGDAVFYTLRYSTDPALAGAAVVDLRDPLYTPPLGSLAMDTTYYWRVTA
                     RDVYSAETDSAVENFRLFRHKAKSPDRLLSVETVSGRPPDGYLFFEDARVSQGELIQR
                     ANRDSLGDRLLRTIRSPAWNVQVRDLSGRVLPSDDVESRLVFRYSASPSAARGAPVLE
                     ADYVKIVSLNGPQGRWELIPRQEAAGSGEARGSVRGFSVFSLVASPSVLAPLSSVTNF
                     PNPFAAGRETTRIRYALTEDAHVTLRIYTLWGDLVREYEAEKGAPGGVGTAVGYTNEV
                     LWDGRNGRDEVVADGMYLAEIRAESSSGARREIRRIGVVK"
     gene            complement(13426..13806)
                     /locus_tag="P5079_04370"
     CDS             complement(13426..13806)
                     /locus_tag="P5079_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29256.1"
                     /translation="MTTGDKKTVLIVDDEPDVVFVLKTALEKNGYAAETAANGVEALE
                     KVPSLRPDAIVLDLMMPKLDGISVNARLKENPETREIPVFVVTGKGHLRELLEVRQDF
                     RVAAYLEKPFRVAQLLEKLREVLG"
     gene            complement(13803..>14616)
                     /locus_tag="P5079_04375"
     CDS             complement(13803..>14616)
                     /locus_tag="P5079_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY29257.1"
                     /translation="EEIVAVAKRCSDHEEQRLEMGRLRKTIVRHQELDRLKSEFVSNV
                     SHELRTPLFAMGAALELLWDGPSDSATSAKLREVVQNNYDRLREMVGNVLDFSRMERG
                     TLRPVFLPVDITELTRRTEKDLRPLFTKNGISVEPLPEGLPSLMAEVDAGQIQQVLVN
                     LLGNAAKFTPPGGKVGIELSEQDGRVRLSVWDTGKGVAPEHQGKIFERFYQVDSSDTR
                     EAGGSGIGLAIVDGIVRMHGGHVWMESRLGNGSRFHVELPKHRETTGAEGKT"
BASE COUNT         2989 a         4827 c         4222 g         2578 t
ORIGIN      
        1 attcaaaacg ttcttcaaca gctggtggac ccccgcatcc acgtctctcc ggaattttaa
       61 ccggccgacg gcgcctttcc ctcctccatc agcggcagga ccgtccgcac gacggtccct
      121 ttccccctct cgctctccat cgacaaccgg ccgccgtggc tccggatgat ctcctgggcg
      181 atcaccaaac ccaggcctgt gccgtcctcc ttggtcgaat ggaaccctcc caaaatcttt
      241 tgaatcctct cctcggaaat gccggggccg ttgtcgcaga actcgatgac gcattccccc
      301 ttctcccggc gaagcgccac cgtcacccgc cggaccttct gcccttccag cgcctcgacg
      361 gcattcttgg cgatgttgaa atacacctgc tgaaggctgt ccttgttgcc gaaaacacgc
      421 agatcgccgt cccccggaaa agccgtttcg aaccggacgc cctttgattt ggggtggaac
      481 tggatcacgg tgacgatcgt ctccaggagc cccgccggac ggatctcgct tttttcctcc
      541 tcccccggtt tcacaaaatg gagcatccgc tgtatcagct tggagatgcg gtcggcctcg
      601 ccgacgatga tctcgaaatc cttccgggcc tccgcgtcga gattctcgtt ctgtcgatgg
      661 atctcgacga aactgctgat ggaagagagg gggccgtgga tatcgtgggc gatcttggtc
      721 accatggcgc cgatctgcga cgtggcctcc aaatgcccca tccgctgcac caactgttct
      781 ttctccctct gttcatcggc gacgcgtttc tgcacatttc gataaatgat gtcgtagtaa
      841 agggccgaga cgatgagaaa cccgacggtc gtcaccatca ccaggagcgt ggcgaaccaa
      901 ctgccgtaca ccagttccgg agaaaccgtc cgatacggga tccgccccga aaactgcgcc
      961 aggaggaccg ccacgtacgt caggatggtc gccggtaaaa tccaccggtg gcgcaattcg
     1021 ctgacgcttc cgaggatgat gatgcaggaa aaaacgaaca cgaaggggga caggatgccg
     1081 ccggtgtaaa aaacggcgac cccgagaagg aaaacgttga ggtaactcat ccacaagggg
     1141 acccaggact tcaaccgggg atagaaaaca tacaggatca ggtggaggac cgccgacacc
     1201 accgccacga tcccgaaccg ccggtcgaac ggccaccggg gaaggaacac cagcagggag
     1261 gcggaggccg tcagaagcac attgaagagg atgtggttcc gcatgatctg cggaaacggg
     1321 acctggaatt gatttgcgat gggcgacggt tttgacggcg agtcgggctc tgtcaacttt
     1381 tacccttttg aatcctgttt tttcgtgtat ttgaccgcca acaaatacag cgaaagtccc
     1441 gccggaggag catagaggta gaaattcacg tcttccacgg ggatccccca gaacttgatc
     1501 cccatgatgg gattgtagat ccaccaatcg tatttgaacg cgaaatagtc ccagatcaac
     1561 cccacgagga acatgaaccc cgtgatgagc gccatcgccc gggggttcac gtaccgccag
     1621 acggcctcgc acagaacgat ggccaggccg ttatagacga taaaatggct gtagaggtag
     1681 tagggctttt cgtcgcgata gaggaacgcc aggacggcga agaccagcgt gccggaatag
     1741 agatagaggc ggtaggacca gggggatttc aaggtctgcg ggcgggcgcg gtcgatgacg
     1801 ttataaaaac cgatcatgaa cagcatcacc atggggtaaa aaagcatctc ctccagggga
     1861 aggttcggca agagccagat gccgatgagc catttgcgcg ggaagatcca ccactgctgg
     1921 gcggagaggg taatgctcca ggcccacccg atcagcgtca cgagggcaaa gccgaggaag
     1981 accgccttcg cgttggcgcc cctgaaaagc caccgccaca acaccagcag cggcaggatg
     2041 aaaaccccga ccaggacctg aagatagcgg ggccgcggcg tatcggcggc cgtcgtggga
     2101 tctcgacgat agtcgcggcg ttccggcatg gcgggactta cccctcaggg aagatcggtc
     2161 cgggtgtggc gtccttcaaa agccccctgc caatagcgga cgaacgtcgg acccagcagc
     2221 atcagcaggc aatagcccat cagcgcctcg tagggcatgt tgaaaaacgg gatggttccc
     2281 cagttccact gcaacacgtc gtaattgtaa tcccaaacgc ccgacttgag cccgacggtt
     2341 tccagcggaa gccccaccaa agcggccccc aagacgtcga agagaaatat ctttttgtcg
     2401 taggggtcga tggcggtttt tttcaatatc cagcaataca gtttctcgga aacaaaaacg
     2461 accagcgtga acatcacgcc ccagcccagg accacggaca acggcacgtc cttgtaaatg
     2521 gtgaggcgga aatgataggt ccagaggggc tcggtggaaa attcgatgaa cgccccgatc
     2581 atggcgccgg ccaccatgtc cccgaaaacg tttttcttcg caaaacggcg aaagagatac
     2641 caaatgaccg gaagctgaag ataggaccat atttcccata cgatcagttt catgaatttt
     2701 tcctcccttc aatcttgtgt ttcacaaaca gcataaaaac gatgacgaag agccccggga
     2761 accagtaata gaggaggggt tcctcgaggg ggacgccgaa gaccttcggg ccccaaatgc
     2821 gggcctcgtt gtagacccag tggccgcgtt tgaggctgta atgctcggcg aaaagcatga
     2881 aggtctcaaa acagaggatg gtcgccaggg cggcccggcc gttgagctct ttgcgcgcca
     2941 cggagtaaaa aggcttgaag aggaacagga agggcaggag gaggagcgag aacgccggcc
     3001 accaggcgcc gcggcggtag gtggggaact cctccttctt tttcccctcc ggcggcaggg
     3061 gttgtatgtg ttcaggcatg ggggaccctc ctgaaaaacc gccggtccag cagatcgaag
     3121 gccaggtaaa gcagcatgat gacgccgcag gcgccgagcc agaagaagaa ttcctccacg
     3181 ggggccccga agagcgtcca gccgatcaac gggtcccacg cctcggaaaa gttccacacg
     3241 tcggcccaga gatagacgta ttccaccacc aaggaggtcg gaaggcaaag gccgaggaac
     3301 gtgatccaga aggctttccg ggtggatttt tccaggaacg gccaaatcgc cggcatcaag
     3361 acaaaggacg gcacccagaa cccgaaaaaa tagaggagga aatagcgcgg cacgttagag
     3421 aagggctttt atttcggaaa ggaaggtctt gggattgacg ggtttggcca tgaagaaatc
     3481 cacccccgac ttcttgatgg cggcgatctc ctccggatcc ttggcggcgg tgagcgccac
     3541 gatcttggtg gtgctcagag tggcgtcggc ccgcacgagg cggacgatct cccggccgtc
     3601 ggtctcgccc agataaatat ccaaaacgac gagttccggc cggaacacct gcatgaggaa
     3661 acccgccttg aacccgacct gggtggtttg cacctccagg aacgggtcct tctccagcag
     3721 acgcttgatg aggcggagtt cgacggcgtt gtcctccacc accaggattt tcttccgccg
     3781 ggtgtgttcg ggggtgtagg gcaggccgtt gtccttggcg aatttctcca aatcagccgg
     3841 ggcgatgcgg aaatgcccgc cgggagtgga atacgcgttc aactttttct gcagggcggc
     3901 gcggaaaatc gtggagtggc tcacatggca cagccgggcg gcctccgccg ttgtgtaata
     3961 tttctcaaat tgctttttgg aatccatatc cccatctccc tctatttaca gtctattgac
     4021 aaaatagatc aaccgtgtta agtttaccat aattccgcga caacacgaca gattcgctcc
     4081 attccatcgg tttctggagg gtctccatga gaaaacgttc gtccatcctt ttctgtcttc
     4141 tttttttcct cgcctccctg ccccgcatgg cccgcgccgc cggatacgaa ttcgaaggga
     4201 tcggcgcaag gcaggtcggg cgggcgggcg cggccgtggc cgacgtgtcc gattggacgg
     4261 ccatttattg gaacccggcg ggcatggccg ccccccgact cgacggcgaa acgaacgcgg
     4321 gacaggtggg gttcgaagcc ttcggcgggt atgcccgcac gaaagacggc aactccctct
     4381 cgcgcctggc cggccccctg ttcaacgaaa aacatatggc gtcctccttc atcctcggcg
     4441 cgttcggcgt ccgggtcccc cttgaatccg gatgggtcct gggaacgggc gtctacaccc
     4501 ccctcatcca gggagccgac ttcaacgaca ccgccaacgg aatgacgctc gattatttcg
     4561 cgcaggccgg catcgccgtg gtcaatttct ccgtggcccg ggtcctctgg aacgacgtca
     4621 ccctgggggc cggggtgaac gcggtgtacg cgcagatgcg gagcaactcc cgcctcacca
     4681 attccctgag cggcaccgtg gccaactccg acatcgccgg ggacggctgg gggccggaag
     4741 gcatcctcgg gttgcagtgg cggatgtcct ccgcctggcg gttcggcgcg gtctatcgca
     4801 cggggtcgcg catccccctc aaaggggacg ccgacgcctc catgaccggc ctccccgacg
     4861 agcgttccac cttcgggtac gatttcaacc acccggccac cgccgccgtg ggggtctcct
     4921 tcacgcccgt gccgaaggcc acgctctcct tcgacatcaa ccacaccaac tggcgttcct
     4981 tccgtggaga catggacttt gaaaccagcg gccccctgtt gagcgacatc cggaactcct
     5041 acgcctggaa aaacaccttc aagttccggc tgggcggcct ctatcgcctg acggacaaaa
     5101 ccgacctgct cgcgggactc tcctacgaca actacgccct ggacaggaac agcatcgatt
     5161 tttccaccac catcgacgtc cccattttcc gttatttcgg cggggtgacc cgccggctcc
     5221 cgaaaggatg ggaaatgagc gccagcctgc tggccgccaa gggccgccgc gaaaagaacg
     5281 gggtggatta ctccatcggc ggatggcagg cgatggtgga aaccgcctgg aatttctcga
     5341 cgaaacatcc ataaatccgg aggcgccatg aacgacctga tccagaccat cgaattcttc
     5401 gccgtcttct ccatggcgat cgaaatgggg ttcgccacgc tgttttccct gcctcccgtg
     5461 tacgcctttt tcaaaaagaa cccggacaaa aagtatctga aacgcctgct ggtgctggcc
     5521 ttctgtctcc tcctctgcgc cgccctttac aaagccgtct ccgccgacaa gggcgccctc
     5581 ttcctggaga ggatcttcgg ccaaaaagtg cccgtggtgg acgtcctcct ctccgccctg
     5641 atcctcgccg gcgggtcccg ggccatccac gacatcctta aaagactcag cggcgccacc
     5701 gctcagaaac tcctacaaca gggaaaaccc tgattcccgc acccgccgtt tctggggcgc
     5761 gccgaacctc tcggcacccg aggctcctca aaatatccag cgccctcctc ctggcggcgt
     5821 tcggcacggc caccgccctg caggcccgtc cccgcccacc gaaaaacatc atcctcctcg
     5881 gctgggacgc ctgtcaacgg aaccatctga aggaatgcct gtcccgcgag gaactccccc
     5941 acctcaaaaa actgatctcc gaggggaccg tcgtggcgat cgacgtcctc cggcacaccg
     6001 acaccaaggc gggctggacc cagatactga cgggctacga gccggaaatc accggggttt
     6061 attccaacaa cagattcggg ccgatccccc cgggttattc cgttttcgaa cgtctggaaa
     6121 acttcttcgg gcgcgacaac atcgtcacca tggtcttttc cgggaaaagg atccatttcg
     6181 actcgacccc cccggccaaa atcccctata aggacgccga tcaacttcct cccgacgatg
     6241 tgtcgatgta caaaacggac accggcggcg gcgcgctcat caccgaaggc ggcgaaaaat
     6301 actgggacgt ccccgcccaa ccctatcatc tcatggccaa ggccgtggac gtgtgggaaa
     6361 acgcgctggg gctcaacgac gacgtcacgt ctcccctgct ggaagcgatc cgtcgccaca
     6421 aaaacgaacg tttctttatc ttcgccctgt tcaacgacat cgactatttc ggccacgagt
     6481 tcggcgagaa ctccccgcag tacaacgacg ccatcgtctc cgccgacgcg tgcctcggcc
     6541 ggatcgtttc caccctccgg gagctggggc tgtacgacga cacgcgcatc tacatcaccg
     6601 ccgaccatgg attcgacgag gggaagaaaa atcacaacga cgccccttat gtcttcctcg
     6661 ccaccaacga tccccgggtc attcggcggg gcgaacggag ggacatcacc cccaccctgc
     6721 tggaccgcct gggggtccct ctggaaggga tcaccccgcc cctcgacggc cgccccctca
     6781 cgaaacgtca taaaccgccc cgctggtaag tcttccccct caaaacaccc aatcggcttt
     6841 cgatttctcc tcttccaata tctccgcgat ccgtcgacgg cattcctccg gaagtcccgt
     6901 cccgtcgttc cgtaattttt tcagatgttt caaaacttcc ctctgcacct cggggctttc
     6961 gtcccgttcc agctcgaaaa ggacgtccac ggccgccgga tcgccggttt ccgccagcgc
     7021 cggcacaatg aggagccgcg tggtgggatt ggcgtccttc gacagccccc gcaggaagcg
     7081 gagggcctcc cggggcgccg tttcgtagag gttcctgacc tgggcggata tccggccggc
     7141 ctccacctcc cgctccggag gggtcacgtc caccaacacg tcccggatct cctgttctct
     7201 ccgatgtaaa aacatctgtt ccaggagggt gccccgttgt ttgcccgccg gcccctctgt
     7261 ttttccggga accggcgcgg cctcctgttc caatccccgg tcaaaccgac gcatcaaccg
     7321 ttccagttcc tccttatgcg tcgcctccat ccgacgcagc atgtccgact gggtctccga
     7381 tttcgcctga agggcggccc gttccgtgtc caaccggttc agtttctccc gcaccgactt
     7441 catctccgtt tccaaagcct tctgccggtt tttctgcagg aggacggcga cccccaaaaa
     7501 caggatcact cccagggaca cataacggcc ttttgtctct tccgccttct cgcgtgacga
     7561 ggaaggagcc ggggcgggcg cgggggacgg gacggaagcc ggcgccgtcc ggacgaccgc
     7621 cggccctgtc gccgcgcggg gcgccgcctt gggcggggcg ggcgcgggag gaggagcggc
     7681 gggaggagga accgccttgc ggacggagtc cgaaagggcg gacaactccg aatcgaacgg
     7741 aaaatatctt ttcgcctcct ccacgtattc ccacgcccgc cggtagttgc gtttctcgaa
     7801 ctggtcgtgg atggcccgaa aatagacctt caacagaagc cgctgggcct tctcgtcgcc
     7861 gccgttggcc tccaacgcct tcttcaacga tccgatggcg gcctcatcgt tgcggtcgat
     7921 gtattccttc acgccctgct gaaaaaaacc cgctccttcc ggtttcaccg gggtgtcccc
     7981 cccgacgcgc gacgcgatcg ggacggaagg gaccgccaag aggcccgtct gttggtcgtc
     8041 ggcgagaagc ccggcgaccg acaagaagag aacccctgcc aaaagaaacg accgtttccg
     8101 tttcatggcg tccccttggc ggccgccggc tccgaaagtt ccaaaacgtc gcggagcctc
     8161 tcgtagagcc gttgggcctc ctgattttcc ggcgaaatcc taacgacttt caacaaatgt
     8221 ttttcggccc cctcgtaatt cagttcctcc aggtagatct gcgcctgcgt caggctggcc
     8281 aacacctggg cgtcggcgaa ttcgcggtcg cggaggaggg ccattttggc catctccaaa
     8341 gcttcccggt actgtccggc gtcgtagaaa cccttcgcct gccggcaaaa cccttccgcg
     8401 gcgatccggg cctcgcgccg ctcgatctcc cggcggagat tcaacgccgt tggatcctcc
     8461 ggccgttcgt ccagcacgcg ccggagccgt cgtttgctct cgtcgtattt cgcgttttcg
     8521 aaaaggagct gggcctccaa aaggagggtc ctcaactccg cctcgcgcgc caaccggact
     8581 ttttcctttt ggacctcgtc cttcatggcg tccagttcgg cctcccgcgc atggactttc
     8641 cgccattccg acgccaactt cttctcggcg agaggcgaga ggacgccgta gcgcaggctg
     8701 atgccgaaat gatgaacgac cgccagctca tgaaaagagg cggcgtaatc aaaacgcgcc
     8761 ggccccttct cgatccccca tccgaaagag tattccctgt ggttgaaccc cacgcgcaag
     8821 gtcagaggca ggggcgcggt gggcaacagc agagcctcca acccgccgcc ccaacgggcg
     8881 atccgccggc cccccaagcc gtgatagacg tcgtattcgc cggtccagga aaaatgccgg
     8941 ccgaaaatcc ggtagccgta cgtccctccc aggcgcatca ccgccgggaa ccgatcggtg
     9001 gcggtattga gccgaagttc gggcggcacg aggttctgaa cggcggcgcc gcaggaaaaa
     9061 gattcggaga gtttcaccct cacccccgcg tccagcccca cgccggtggc cgaatattcc
     9121 gccaccgact gggacaccgc tttcaagttg acccccccgc tccatcgctc tgaaacctgc
     9181 gccccgtagg aaaggaggaa cgcgaaatcc tgctcgtcga aacttccctg ggactgtccc
     9241 aggaaatccg ttttctccgc ctcacccgag ccgagatgaa ggagcgtcac gccgacgctc
     9301 tgtttcggcg tgaggggatg cgccaccgcc aggctctgga attggccggc ggagaaaagc
     9361 ggcgacatgg agaacaccgc ctctcccatg ggattccccg caatgcccgc ggggttggcg
     9421 taagcggacg ccgctcccgg aaacgacacc gaggcgttcc ccaacccggc gtcccgggcg
     9481 ctggcggcca acgccgtgag ccactcgccg gccaaaccgg cgttggccgg ccggtcgcgg
     9541 aacggcacat tcacgccggc ccagacgggg acgccgcaaa agagaagcag gggcaaaagc
     9601 cgcctcactt gaccaccccg atccggcgga tctcccgacg cgcgccggaa gacgattccg
     9661 cccggatctc ggccaaatac atgccgtcgg ccaccacctc gtcccggccg ttccggccgt
     9721 cccaaaggac ctcgttggta tatcccaccg ccgttccgac gccgccgggg gcccctttct
     9781 ccgcttcata ttcgcgcacc aaatcccccc acagcgtgta gatgcggagg gtgacgtggg
     9841 cgtcctccgt gagcgcgtac cgaatgcgcg tggtctcccg gccggcggcg aacggattgg
     9901 ggaaattcgt cacgctcgaa agcggcgcca agaccgaagg ggaggccaca aggctgaaca
     9961 cggaaaagcc ccggaccgaa ccgcgcgcct cgcccgaacc cgccgcctcc tggcggggga
    10021 tcagttccca gcgcccctgc ggaccgttga gcgaaacgat cttcacgtaa tccgcctcca
    10081 gcaccggcgc gccccgagca gccgatggcg acgcggaata ccggaagacg aggcggcttt
    10141 ccacatcgtc gctggggagc acgcggccgg agagatcccg cacctgcacg ttccaggcgg
    10201 gactgcggat cgtccggagc agccggtcac cgagagaatc ccggttcgcc cgctggatca
    10261 actccccttg ggagacccgg gcgtcctcaa aaaaaagata cccgtccgga ggccgtcccg
    10321 agaccgtctc cacggacagg agccggtccg gactcttcgc cttatgtcgg aaaaggcgga
    10381 aattttccac cgcgctgtcc gtctccgccg aatacacgtc ccgcgccgtc acccgccagt
    10441 aataggtggt gtccatggcc agactgccga gcggcggggt gtagagagga tcgcgcagat
    10501 cgacgacggc ggcgccggcc agggcggggt ccgtggaata ccgcagggta taaaacaccg
    10561 cgtctccgtc cggatccacc ccggcctccc acgcgaacga cacgtcaaaa acggcggggt
    10621 cgtcgtccgc cagcggacgg agcaatcgga atggccgggg tgggttggaa ttatggacgg
    10681 cgaactccgg cgtcacgacc gtatgcccca gcggcggttc cccggaatcc gtggcctcca
    10741 acatgagccg gtagccggcc ttgttgggcc attctctcgt tctcaaaaca tacgttgtgg
    10801 cggaggtgtt cagcaccaag gggataaaac ccgcgccggt gtgggtggaa aagtagaggt
    10861 tgaacgtgtg ggtgttgaac gtgttggggt cgagcgcccg ccatcgcacg gcgaaatcgc
    10921 cggaaacggg ctggccgttc agcggagcca aaaaactcgc caccggcggc tcgttcacgt
    10981 tgtccacgcg aaagtccccg tccgacacgg cggaggagac gaacccgccc tggtccaccg
    11041 cctgcacctt gacccggtag gcggttccgt tggaaacgct ccgggaatcc cagaggaaag
    11101 acgtcgtgcc catggggagg ctcgccaaca gggcggggaa cccttggccg ccgtcggggg
    11161 agagagagac cgatacggaa aaagtgccgg ccggattggg atcggaagcg atccaaacga
    11221 tggtggcgat ctgcgtgacg aacgcccccc cgttgggata aacgagcgcc acgtggggcg
    11281 gggcgttggt gaacgccacg ggactgacgg aggaaagcgc ggaaaggttc cccacctcat
    11341 cctcggtttt aacggcgaaa taatacgtgg cggcgtcgct cagcccggaa acggccgttt
    11401 gcaacgcctg ggccggatcc gcggaaaaat tgaacgtccg ttccgcggcc gcctgccaat
    11461 ccggttccgt gagaatggga ccggaaacgg aataccgcac gcggcacaac ccgttgtcga
    11521 cccgcccctt gtcgtctccc gggaaaatcc aggacaggac gaccgacccg ttggccgcgc
    11581 ccatggcggc cgcgagctgc gtcaccggag aaggcggggt gtgggtatcg aggagcaact
    11641 gaaacggcga cgaccaatcg ccgtagagac cggcctcgtc ccggccgcgg acgcgccaat
    11701 accaggtgcc ttcggtcagc aacgtggtgg tggagaacga ggcgtcggca agaccgtccc
    11761 gggaaatatc gggcgacgcg aacccgggat cgtcgtccgc ctggaactga tagcgcaggg
    11821 cggtatcgcc gtcctggtcc gccacgggat tccaggcaaa caccggggtg tcgtcgttga
    11881 gccgagaccg gtcggccgga gagagcagcg tgggccggaa cggcgcccgg ttgaaaacga
    11941 tcttgaactg ggacgcccag ggtcgcgccg gaacatcctc gacggagaac gaacttctca
    12001 gcaagaggaa ccgccggtcc ccgtgtcccg cccaaatggt ctgagccgcg gcggaagcga
    12061 cgtagaaggt ctgcgaagac ccgtccgggc cggaaaaatc gttgaacgtc accccgtcgg
    12121 tcgaagaagc caactgaaaa gaaaccgtgc tcccctccac aacagccgaa agacctcccc
    12181 agcggatcgg cgccggcaga ccggccggaa aactttccca ggaatccacc cggggcgaag
    12241 tccacgtgcc ggtggacgcg aaggtgtaat accgcagaag gttggagctg acgtccctcc
    12301 ttccggcgaa cagcatgccc cgctgacgca ccgtatcgta ggccatgccg tggccgtaac
    12361 gaccgggagg agccccgacc tcgcggtcga actcctgcca acggtctttt ccgaaatgat
    12421 agaaccgaat gtccccgaga tacgtggcga ccgcgtcacg gccgccgaag agaaccgtct
    12481 ggccgtggcg cgcgtcgtac agcagggcgg cctcgctgcg cggcccgggg ggcgaggagg
    12541 gagacttctg ttcccacccc atgccggaga cgtccagcgc ccaagtatcg ccgtagatct
    12601 ccgagaactc ccatccgttc cggcggccgc cgaacaggat tatccgccgg gcgctccggt
    12661 cgtaagccat gcaggaaccc atgcgcacac cgggggaagg cgtcgggctc catcgggacc
    12721 agctcgaggc ggccacgtcg aacacccagg tgtgggcgtt gaccgtaatc ccctcaaccc
    12781 cctccccgcc gaagagcacc actttgtcct ggtccgcgtc gtaggccatg gcaaagaaag
    12841 cccgcgcctc gggggcgtcc ggagaggcca caaaggtcca tgtgtcctga accacgtcgt
    12901 acatccaggt gtcgtttaaa taaccgcccg acgcatcgag cccgccgaaa aggaggaatt
    12961 tatcccccgc ccagacgagt ccatggccgt agcgcgccgc cgggccgccg gccggaacat
    13021 gttccgtcca gccggaggag ggatcataca cccacgtatc atcgaatttc agatcgttct
    13081 gatcgtgccc gccgaagacg accgctttcc cacgggcctc gtcgaaggcg aaacccgcgt
    13141 ggtagcgcgc cggcatgggc gaggaggcgg acccccacag cccggccaaa gtcaaatacc
    13201 ccgcctccac ttccgtgtcc gatgtttgcc ctgaaaaaaa atttcccgaa tcgaaaatct
    13261 tttccgacgc cccctggccc ccgacgggcg ccagaaagag ggcggcggcg aagaacccgg
    13321 tgaaaccggc tctctccaaa acggacctcc ctgtctcccg gccgatgccc atcgttcact
    13381 ccgttcttga tttgcccgcc tccaaaacat cttccacgga acgccctatc ccaacacttc
    13441 ccgcaacttt tccaaaagtt gcgccacccg aaacggtttc tccaaatagg cggccacgcg
    13501 aaaatcctgc cgcacctcca acaactcccg gagatgcccc ttgccggtga ccacgaaaac
    13561 gggtatttcc cgtgtttccg ggttttcctt caagcgcgcg ttgacggaaa tgccgtccaa
    13621 tttcggcatc atcaggtcca acacgatggc gtcgggccgg agggacggca ccttctccaa
    13681 ggcctcgacg ccgttggcgg ccgtttcggc ggcatacccg ttcttctcca aagccgtctt
    13741 caacacgaac accacgtccg gctcgtcgtc cacgatcaaa accgttttct tatcacccgt
    13801 cgtcatgttt tcccctccgc ccccgtcgtt tccctgtgtt tcggcagctc cacatggaaa
    13861 cggctcccgt ttcccaaacg actctccatc cagacatgtc cgccgtgcat ccgcacgatc
    13921 ccgtcgacga tcgccagccc gatgccggac ccgcccgcct cgcgcgtatc cgaggagtcc
    13981 acctggtaga agcgttcgaa tatctttccc tggtgttccg gtgcgacccc cttccccgtg
    14041 tcccacacgc tcagcctcac ccggccgtcc tgctctgaca attcaatccc caccttcccc
    14101 cccggcgggg tgaatttcgc ggcattcccc agaagattca ccaacacctg ctgtatttgc
    14161 cccgcgtcca cctccgccat cagcgacggc agtccctccg gcagaggttc caccgaaatc
    14221 ccgttcttcg taaacaaggg cctcaggtcc ttttcggttc gtcgcgtcag ctcggtgatg
    14281 tccacgggca aaaaaaccgg gcggagcgtc cctctctcca tacgagagaa atcgagcacg
    14341 ttgccgacca tttcccgcag ccggtcgtag ttgttctgca ccacctcccg caatttcgcc
    14401 gatgtcgcgc tgtccgaggg gccgtcccag aggagttcca gggccgcccc catggcgaac
    14461 agaggcgtgc gcaactcatg cgacacgttc gacacgaact ccgacttcag ccggtccagt
    14521 tcctgatggc gcacgatggt ttttcgaagc cgtcccattt ccagacgttg ttcttcgtgg
    14581 tccgaacagc gtttcgccac ggccacgatc tcctcc
//
LOCUS       DAOTFD010000045        14576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2862_length_14576_cov_5.186695, whole genome shotgun sequence.
ACCESSION   DAOTFD010000045 DAOTFD010000000
VERSION     DAOTFD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_14576_cov_5.186695"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="P5079_04380"
     CDS             complement(<1..713)
                     /locus_tag="P5079_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRY29258.1"
                     /translation="MGKFRPYFSIAAGLGLGLLANAARGDGPVAQYAGALGLEPITVL
                     AERYPRMKADLAGTRDVLTRRDIEESRQPEIATLLQQRLFVTVQKPGIGALSGLGIRG
                     ISGGRNNNKTRVLVDGVPTDTLRRGFTLETIDPQTVEQLDLIRGPASALYGGGALNGT
                     LNVMTRVPDRREFAAGAAGGSYDTKTSRLYYQDKVGRVAWRVNANSLDTNGHVQVNGD
                     KMINPAWLTNQGTFLEYAG"
     gene            complement(733..1434)
                     /locus_tag="P5079_04385"
     CDS             complement(733..1434)
                     /locus_tag="P5079_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRY29259.1"
                     /translation="MQNSGARPAEALPITQVRRDFLKIAEAVGITGPRLVSKDSRPVA
                     AVIGIRQYEAFLSATRGPRGGQPAPALIILGAKPCERARAEQTLSIVNRLAANPHPPF
                     SKIIFVYGKDTAPLARQIRFHDVRAVKNEKTAYPLIASVKCALGAFSPDDTHFILAFL
                     SGPLQEDRLRLLSNHLRNVPRGKGIAVLRQNGRPTHPVAFAARYKKYFISTRKELGIP
                     YLLKKFHKDISYLEV"
     gene            complement(1555..2094)
                     /locus_tag="P5079_04390"
     CDS             complement(1555..2094)
                     /locus_tag="P5079_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29260.1"
                     /translation="MTTPEISPRDVRRVQVVNRYATPFAILLVALAIFLPSPIRSVRI
                     IGLSLLAFSILFNLVTVGLIRRRPEILPRLKKLRLYTNLAVNVVLVYELSPYWPPIWL
                     LLVLTPIATAIYASARQSLMTSLLIAFLIMGIHLVHGGGGLMEWGQTLSRAAFVVLLS
                     LLLNELAHEAGLNSPRNQP"
     gene            complement(2123..2551)
                     /locus_tag="P5079_04395"
     CDS             complement(2123..2551)
                     /locus_tag="P5079_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY29261.1"
                     /translation="MRHRRPAVGGFTLIELMIVVAILSILALLVLPKFGALVQKSKEG
                     STKGHLGAVRGALRLYYMDNDQNYPPSFSALSPKYIGNAPPLLYTAQHPLSETVDDLP
                     AMDPVSDAAHWGYVPAGQDAGHFWIQCTHTDTAGTPWSTH"
     gene            complement(2622..2695)
                     /locus_tag="P5079_04400"
     tRNA            complement(2622..2695)
                     /locus_tag="P5079_04400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2659..2661),aa:Glu,seq:ctc)
     gene            complement(2914..3825)
                     /locus_tag="P5079_04405"
     CDS             complement(2914..3825)
                     /locus_tag="P5079_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY29262.1"
                     /translation="MTKTRSIRKVIKARPTMEGAGVHLNRVFGFGTESLFDPFLLMDD
                     FRSDTPAHYLKGFPWHPHRGIETITYVLAGDVEHGDSMKNKGVISAGDVQWMTAGSGI
                     IHQEMPKGDSQGRMYGFQLWANLPKKQKMMAPRYRGVTRDEIPEVVLAQGVKIRIIAG
                     RVGDRQGPVRDVVIDPEYLDVTIPAGGEYVHPTKRGHTVFAYVIEGQGSFCREKDPFT
                     YEAVGVNYFDMKRDPFVSNGMLALFDDGDDVRVSSEKGPVRFLLIAGKPLREPIAWYG
                     PIVMNTEEELRRAFEEFNNGTFIKNPV"
     gene            complement(3926..5356)
                     /locus_tag="P5079_04410"
     CDS             complement(3926..5356)
                     /locus_tag="P5079_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29263.1"
                     /translation="MFNHARAVSLTVSLMLVASFAAAELSSKAKRAEKMAAAGQLTEA
                     MAIYQELMGKNAQKEPQLVKEGREGMANCLLLQAKTAFDEKNFADSKTLALQILTTYK
                     DTPSVNESAKTLALSQLELSKQLVDGKKYDEGIAQCKEVKEKIPAGGDALKADLDKFL
                     SQLAGDLLKLAEGHIKSGKYEEAQKNLEWASAAATDKAASAKCQYTQGVCFRLAGQND
                     KALAAYQDVTTDYPDSPFAATAHADLYLIHTQLDNLPEALKSIQQAASLAPENSDYLF
                     KTAQAYQALGKTEDAKKAAKKAIDLLQAELPKTYTNKENLQYKIGQAQLILGNYTEAA
                     VEFDKALARNADMVEAKKGLALALFNDKNYTGAVDAYDALIKKFSGDFDALNEKASKD
                     KIPADLAKKVEDLRKDIAYFHYQKGLSYEQLGEYDNALAECRLGLEGVSTQEAAATIK
                     RIQTAAAQKKSETAPAPAATTAPVEK"
     gene            complement(5489..7186)
                     /locus_tag="P5079_04415"
     CDS             complement(5489..7186)
                     /locus_tag="P5079_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY29264.1"
                     /translation="MRTNLTTKKVLLTSVCRPFGESAGDAPSVGYELLHKQVTRAQGI
                     FSPRVINSFFSLDYIAANLETPTVVLQYPSRAELIRELEKGYDYVGVSFIMAVFHKMK
                     DTVELIRKHSPKTKIVLGGYGTILEDDLLKPYGDFVCRGEGVGFFRELLGETPLSMPY
                     EHPIMLSTLKLFSIPISRTGQIFAGLGCPNGCDFCCTSHYFGRKHIRLLPTGKDIYDV
                     IERYWKNDPQTVFMLFDEDFLLNKTRAMEFRDCVSKGGRVPSIFVFSSVKAISQYTVE
                     QILEMGIDGIWVGYEGTRSGYAKQQGRPVADIFTEFREHGITILASMIVGFEYQNRDI
                     IAEEHAGLMKLHPALSQFLIYGPVPGTPLYARMEKENRFRDCFKGQPEKYFRKADGFS
                     VMVKHPAMTTDEVEGAQRWCFEQDYQQLGPSIFRVIEAWLLGYRKLKDSSNPLLRKKA
                     AVYAQDLRKSYPIFLSGRCLGPNAAVRRGIGDLQRRVISELGRPTLGERGMSLVALAM
                     ACWTWFTLKFNLFQHPSLTRTTYRMPDNETALPSWEKIQSSLPVGDLQISVEKNPTR"
     gene            complement(7412..8701)
                     /locus_tag="P5079_04420"
     CDS             complement(7412..8701)
                     /locus_tag="P5079_04420"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRY29265.1"
                     /translation="MNLLKEVLSAQVYPAIGCTEPGAVALCASRAAHALGEPVRRAEI
                     FTDRATYKNGVGVLLPNTAGGRGCLLAGTMGLFLAAPHLKMQLLKTCTPSLAQKAARA
                     IRQGRVSFTVLPRKKGIHIEVRVEGRRHRATCIVRDHHNETSLLLRDDTTLIDRSVSG
                     RSASPHFKKILKKISLRGLLRAAEQMDNADAAYIEEGVTTNLKAAEAGMALKKVGFYL
                     KDLRKRGYLLNDVFSSTKIIVSCAVDARMAGCPVPVMCSGESGNQGIVAVLVPHNVGQ
                     AFKISRRRILKSIALSHLVNAYVKAQIGELSPLCGCAIAAGVGAAAAVAYQQKGGDLK
                     AITLAINNVVSDIGGMLCDGAKSGCALKVVSSADCAIRSAYMAARHYGITAIEGFVGH
                     TAEETIRNLAKIATVGMAQVDDTLVDIMRRKRGRLKN"
     gene            complement(8744..9109)
                     /locus_tag="P5079_04425"
     CDS             complement(8744..9109)
                     /locus_tag="P5079_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRY29266.1"
                     /translation="MKLCLPVLKNAGFSSPICAHFGSAPFFLIVDTETLSLKTIPNAN
                     QHHAHGMCQPLAVLGKEKFDGIVVGGIGAGALNRLRSAGIAVYKTAFPSLKETVSAYN
                     QGQLPEVTPDMACAHHHGE"
     gene            complement(9106..9399)
                     /locus_tag="P5079_04430"
     CDS             complement(9106..9399)
                     /locus_tag="P5079_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRY29267.1"
                     /translation="MRPRKCRRIGCLVNSNYFKPRGIPLVELEETVLRPDELEAVRLA
                     DLNGLYQEEAAGQMRISRQTFGRIIDSAHKKIADALLGGKALKIEIPLKGDAP"
     gene            complement(9478..9666)
                     /locus_tag="P5079_04435"
     CDS             complement(9478..9666)
                     /locus_tag="P5079_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29268.1"
                     /translation="MEQQTRKAFQEIIFALLYIILFLVLMPPLIRLLDDLWGKILYGV
                     LVAGAVAVAFRLRALSRR"
     gene            complement(9650..10318)
                     /locus_tag="P5079_04440"
     CDS             complement(9650..10318)
                     /locus_tag="P5079_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY29269.1"
                     /translation="MTEPGAIMETAKETPPRPHPHPEPRLNRFLYWLGHTFFFTVYKL
                     VWRLKIVGRENIPLEGPLILAANHASWADPPVIGCSLPRPIYFMGKQELFDVPLFGGI
                     IRRVNAFPVKRTEHDVGAFKTAQRLIISGKAMIVFPEGKRQRTGKLAAAKAGVGMLAA
                     KTNAPVVPVYVHNTYRLWSLPRLWVCFGAPLSAGGGKEYQAFSDRVMKEIVRLKETHL
                     GTTN"
     gene            complement(10311..10997)
                     /gene="cmk"
                     /locus_tag="P5079_04445"
     CDS             complement(10311..10997)
                     /gene="cmk"
                     /locus_tag="P5079_04445"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRY29270.1"
                     /translation="MPRKSVIAIDGPAGAGKSTIAKLLSQELGYRYVDTGAMYRALTW
                     KALRDRVPFHSPEALSSLAKKTKLRFVQKKGVSRMLADGEDVTGRIRTQRVTRLTNTL
                     AAVKGVRQVLRQRQREMGREGGVVMEGRDIGTAVFPQADFKFYLDASPMERALRRYRE
                     LKAKNKRVSLDSIAEAIRQRDYKDRTRGISPLKVAPGATVIDTTKMTLHDVFKFILNH
                     VRREGRNGND"
     gene            complement(11020..12333)
                     /gene="aroA"
                     /locus_tag="P5079_04450"
     CDS             complement(11020..12333)
                     /gene="aroA"
                     /locus_tag="P5079_04450"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRY29271.1"
                     /translation="MTVSTSSRIVVPGGSLRGETLPPPDKSITHRAIILSALSTGTSR
                     IIHPLAADDCERTAEAFRSLGISVERDRTGAWVVVGRGLYGLNEAPSQTIYCGNSGTT
                     MRLVSGVLAGQPFETKLMGDHSLSNRPMDRVAQPLRAMGAEIAARDGKYAPLHIHGRR
                     PLTPVDWKSPVPSAQVKSCVLLAGLYTEGITSVEETFKSRDHTERMFSAAGIKLDIQG
                     NRVAVRGGQTLTAQEWVVPSDISSAAFPLVAALIAPGSEILLRNVDVNPTRDGLLEVL
                     QAMGANIKREDIRVMGGEPVCDLVVKTSGLKATRVDEEIIPRLLDEIPILTVAATQAE
                     GVTEIRGAAELRVKESDRLAHVTSELTKMGARIQELPDGLIIRGPTPLRGAEVDSFND
                     HRMAMAFAVAGLIADGNTSIRNADCVDISFPTFWEEFEKLRVATT"
     gene            complement(12335..13186)
                     /locus_tag="P5079_04455"
     CDS             complement(12335..13186)
                     /locus_tag="P5079_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRY29272.1"
                     /translation="MKPERVAIIGVGLMGGALGLALLRKKRRWHVAGVGRNAQRLRLA
                     RRHGAVQEHTTDLAAGVRDADIVVLAVPVDEIAPLARRIRPHLKTGALVMDVGSVKAP
                     IVRSLQKIFSSSGGPHFVGAHPMAGSEKTGVQHARADLYQDAVCVLTPSGNTPRAALE
                     RARSFWRGAGARVVRLDAVTHDRWMALVSHLPHLLADCLVLAADRGPSARRTLRLLAA
                     GSFRDMTRVAEADPRQWDAIFRMNDKNLKVAAARFQKILRRLVGARNRRIPLAKAGRL
                     HSLLADH"
     gene            complement(13212..14231)
                     /gene="aroF"
                     /locus_tag="P5079_04460"
     CDS             complement(13212..14231)
                     /gene="aroF"
                     /locus_tag="P5079_04460"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRY29273.1"
                     /translation="MIVTLKTGVKKTEIDEVLAKIKELGFTPELSKGTEKIVIGVIGE
                     GNRAIAYRNVFESMEQIETVTPISKPYKMVSREFKKTDTRIDFDGFSIGGKNIIVMAG
                     PCSVDTRDNLLRVAKELKKMGAHILRGGAFKPRTSPYAFQGLGEEALRYLAEARKATG
                     MPFVTEVMDTRQVELLERYADIYQVGARNAQNFELLKELGSSRKPVLLKRGMATTVEE
                     WLMSAEYVVAKGNPNVILCERGIRTFETATRFTLDLNAVPVLKHLSHLPVFVDPSHGV
                     GVRDYIAPMARAAIAAGADGIIVEVHPNPATALSDAHQALLPVHMAQLMKELRGIAAA
                     VGREL"
     gene            complement(14228..>14576)
                     /locus_tag="P5079_04465"
     CDS             complement(14228..>14576)
                     /locus_tag="P5079_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY29274.1"
                     /translation="PPFNISYAAQVAAAAALADKNQVRRSLRLVKTEMARLEAAFRKM
                     DVAYVPSAGNFLLIDVSPHKGADVFAALLKKGVIVRAVAEYGLPHYLRVTVGRPEENT
                     LFLKAFQEVRQSP"
BASE COUNT         2483 a         4076 c         4978 g         3039 t
ORIGIN      
        1 tccccggcgt attcgaggaa cgtcccctgg tttgtcagcc aggccgggtt gatcatcttg
       61 tcgccgttca cctgcacgtg gccgttggtg tcgagggaat tggcattgac gcgccaggcg
      121 acacgcccga ctttgtcctg ataatacagg cgcgaggtct tggtgtcgta gctgccgccg
      181 gcggcgccgg cggcgaattc gcggcggtcc gggacccgcg tcatcacgtt gagcgtgccg
      241 ttgagggcgc cgccgccgta cagggcggag gccggcccgc ggatcaagtc cagctgttcc
      301 acggtctgcg ggtcgatggt ctccagggtg aacccccggc gcagggtgtc cgtggggacg
      361 ccgtccacga ggacccgggt cttgttgttg ttccttccgc cggagatgcc gcggatcccc
      421 aggccggaaa gggccccgat gccgggtttt tggacggtga cgaagagtct ctgctgaagg
      481 agcgtggcga tctcgggttg gcggctttct tcgatgtccc ggcgcgtcaa gacatcgcgc
      541 gtgccggcca ggtcggcttt catgcgcgga tagcgttcgg ccagaacggt gatcggttcc
      601 aggccgagcg cgccggcgta ttgagcgacc ggcccgtcgc cccgggccgc attcgccagg
      661 aggcccagac cgagcccggc ggcgatggaa aaatacggac ggaacttgcc catggatgtt
      721 ttccccttgg agttacactt ccaaataaga gatgtcttta tggaatttct tgaggagata
      781 ggggatcccc aattctttcc gggtggagat gaaatatttt ttgtaacggg cggcgaaggc
      841 caccgggtgc gtgggccgtc cgttctggcg caataccgcg atgcctttcc cgcggggaac
      901 gttgcgcagg tgattggaca acagacgcag ccggtcttcc tggagggggc cggagaggaa
      961 ggcgaggatg aaatgggtgt cgtccgggga gaaggctccc agggcacact tgaccgaagc
     1021 gatgaggggg taggcggttt tttcgttctt gacggcgcgc acgtcgtgga accggatttg
     1081 gcgggcgagg ggggcggtgt ctttgccgta gacgaaaatg atttttgaaa aaggcggatg
     1141 ggggttcgcc gcgagccggt tgacgatcga gagggtctgt tcggcccggg cccgttcgca
     1201 cggtttggcg cccaggatga tcagcgccgg agcgggttgg ccgccccggg ggccgcgtgt
     1261 cgcgctcagg aaggcttcgt attggcggat gccgatcacg gcggccaccg ggcgagagtc
     1321 cttgctgacg agccgggggc cggtgattcc gacggcctcg gcgattttga ggaagtcgcg
     1381 gcggacctgg gtgatgggaa gcgcctcggc cggccgggcg ccgctatttt gcattttagt
     1441 gctcattaaa atgcattatg gcttatgggg ggcgggggag tcaagggggc ggagccgcaa
     1501 cgaggccgtt cccccggcag acgctcctcg gccgccgaag gatgcgtgga aaagttacgg
     1561 ttggttccgg ggggagttca gccccgcttc gtgggccagt tcgttgagga gaagactcaa
     1621 aaggacgacg aaggccgcgc gtgacagcgt ttggccccat tccatcaggc ctccgccgcc
     1681 gtggacgagg tggatgccca tgatcagaaa cgcgatgagc agggaggtca tgaggctctg
     1741 ccgtgcgctg gcgtagatgg ccgtggcgat cggggtcagc accaagagca gccagatggg
     1801 gggccagtag gggctcaact catagacgag cacgacgttc accgccaggt tggtgtacaa
     1861 gcgcagtttc ttgagacggg gaagtatttc cggccgccgg cggataagac cgaccgtcac
     1921 gaggttgaag aggatgctga aagccaggag gctcagaccg atgatccgaa cggaacggat
     1981 gggagaaggc aggaagatgg ccagcgccac caggaggatg gcgaacggcg tggcgtaacg
     2041 attgaccact tggactctgc ggacgtcgcg gggcgaaatt tccggggttg tcatgacggg
     2101 tctcccttga tgaaaatatt tttcaatggg tgctccacgg cgttcccgcc gtgtcggtat
     2161 gggtgcattg gatccaaaag tggccggcgt cctgtcccgc ggggacatat ccccaatggg
     2221 ccgcgtcgga aaccgggtcc atggccggca gatcgtccac ggtttccgag agggggtgtt
     2281 gcgccgtata aaggaggggc ggggcgttgc cgatatactt tggggagagg gcgctgaaag
     2341 agggtggata attctggtcg ttgtccatgt agtagagccg gagggcgccc cgcacggctc
     2401 ccaggtggcc tttggtggac ccttccttcg atttttggac cagcgcgccg aatttgggga
     2461 ggacaagcag ggcgaggatg gacaagatgg ccacgacaat catcagctcg atcagggtaa
     2521 acccgccgac cgctggacgc cgatgtcgca tggggctcct tttcctcgtg tccgtcgtcc
     2581 gagacgaaca agtgtcttgg tttttctgga tggtccctga atggccccaa cgggattcga
     2641 acccgtgatc tctgccttga gagggcagcg tcctaggccg ctagacgatg gggcccttca
     2701 ggaaccagat tgttgcataa cccagacacg cgcggctccg ccgcgaaccc gggataactt
     2761 ctttcggaag tcccgcgaaa acactgggcc cggtccgcgg gaggacgggg ccttccgcgg
     2821 actttgaaga gggggaggag attttagcag attttcgaga cgattctcgg cgagccgatc
     2881 cgcttccttt cagcggacgc cccgggtgtt ttcctacacc gggtttttaa tgaaggtgcc
     2941 gttgttgaat tcttcaaacg cccggcggag ttcctcctcc gtgttcatga caatggggcc
     3001 gtaccaggca atgggttcgc ggagaggctt ccccgcaatc agaagaaacc gcacgggacc
     3061 cttttcggag gacacccgca cgtcgtcccc gtcatcgaag agggccaaca tgccgttgct
     3121 cacgaagggg tcccgtttca tatcgaaata attcacgccc accgcttcgt aggtgaaggg
     3181 gtctttctcc cggcaaaagg agccctgtcc ttcaatcacg taggcgaaga cggtgtgccc
     3241 gcgcttggtc ggatggacgt attccccgcc ggcggggatg gtcacatcca gatattccgg
     3301 atcgatcaca acgtcccgaa cggggccctg tctgtctccc acccggccgg cgatgatccg
     3361 tattttgacc ccctgggcca ggaccacctc cgggatttca tcgcgggtca cgccccggta
     3421 ccgaggcgcc atcatttttt gttttttggg gaggttggcc cacagttgaa agccgtacat
     3481 ccggccttgg gaatccccct tgggcatttc ttggtggata atgccgctgc ccgcggtcat
     3541 ccattggacg tctccggcgg aaatcacgcc tttgtttttc atgctgtccc cgtgctccac
     3601 gtcgccggca aggacatagg taatggtttc aatcccccgg tggggatgcc aggggaaccc
     3661 ttttaaataa tgggccgggg tgtccgagcg aaaatcgtcc atgagaagaa acggatcgaa
     3721 caaggactcc gttccgaagc cgaacacgcg gttcaaatgc acgccggccc cctccattgt
     3781 cggcctggct ttgatcacct tccggatcga tcttgtcttc gtcatcgacg ggtcacctca
     3841 tttaaacggg tcatgttcgg gggaatgcca tcgggcgaca gggctgacgg ggggcgtggc
     3901 atttcaaaaa cggaataacg cgggcctact tctccacggg cgccgtcgtc gcggccggcg
     3961 ccggggcggt ctccgatttt ttctgcgcgg cggcggtctg gatgcgcttg atcgtggcgg
     4021 cggcttcctg cgtggagacg ccttccagcc ccagtcggca ttcggccagg gcgttgtcgt
     4081 actcgccgag ctgttcgtag gacaggccct tctggtaatg gaaataggcg atgtcttttc
     4141 ggaggtcttc cacctttttt gccagatccg ccgggatttt gtccttggac gctttttcgt
     4201 tcagcgcgtc gaaatcgccg gagaactttt ttatgagggc gtcgtaggcg tcgaccgccc
     4261 cggtgtagtt tttgtcgttg aacaaggcca gggcgagccc ttttttcgcc tccaccatgt
     4321 ccgcgttccg cgccagcgct ttgtcgaact cgaccgccgc ctccgtgtag ttccccagga
     4381 tgagctgcgc ctgtccgatc ttatattgga ggttctcctt gttggtatag gtcttgggga
     4441 gttcggcttg gaggaggtcg atcgctttct tcgcggcttt cttggcgtcc tccgttttac
     4501 ccagggcctg atacgcctga gctgttttga agagatagtc gctgttctcc ggggcgagag
     4561 acgccgcctg ttggatgctt ttgagagctt cgggcaggtt gtcgagctgg gtatggatca
     4621 agtacaggtc ggcatgggcg gttgcggcga aaggggagtc ggggtagtcg gtggtcacgt
     4681 cctgataggc ggcgagggcc ttgtcgttct ggccggccag ccggaaacac acgccctggg
     4741 tgtattggca cttggcggag gcggccttgt ccgtggcggc ggccgacgcc cactccagat
     4801 ttttttgggc ctcctcgtat ttaccggact tgatgtggcc ctcggccagt ttcagaagat
     4861 cgccggccaa ctgggaaagg aatttgtcca ggtccgcttt gagggcgtcc cctccggcgg
     4921 ggattttttc tttgacttct ttgcattggg cgatgccctc gtcgtatttt tttccgtcga
     4981 cgagttgttt tgagagttca agctgggaca gggccagggt cttggccgac tcgttgacgg
     5041 agggggtgtc cttgtaggtg gtcagtatct gaagggcgag ggttttggaa tccgcgaaat
     5101 ttttttcatc gaaggccgtc ttggcctgga gcagaagaca attcgccatg ccctcccgtc
     5161 cttctttgac gagctgcggt tccttttggg cgttcttccc catcagttcc tgatagatcg
     5221 ccatggcttc cgtcagctgg ccggcggcgg ccattttttc cgctcgtttc gctttggagc
     5281 ttagttccgc cgcggcaaaa ctcgccacca gcatcaaaga gaccgttaaa gagaccgccc
     5341 gggcatggtt gaacatcgga tcctcccaaa agaatcgcgt atggatgcgg agattgtaac
     5401 atgttttttt gtcgaaggaa ttgcggcgga tttccaggaa agcggttttc cgtttcggag
     5461 gccgagcggc ggcggtcccc ctcccggctc agcgggtggg gtttttttct acggaaatct
     5521 gcagatcacc gacgggcagc gacgactgaa ttttctccca ggaaggcaac gccgtctcgt
     5581 tgtccggcat ccgataggtc gtgcgcgtca gagaggggtg ctggaagaga ttgaatttca
     5641 aggtgaacca cgtccagcac gccatggcca gcgccacgag ggacatgccc cgctccccga
     5701 gggtcggacg gccgagttcc gagatcaccc gcctctgtaa atcgccgatc ccgcggcgga
     5761 ccgcggcgtt ggggccgaga cagcggcccg agaggaaaat ggggtagctc ttgcggaggt
     5821 cctgggcgta gacggcggct tttttccgaa ggaggggatt ggaggaatct ttaagctttc
     5881 gataacctaa gagccaggct tcgatgacgc ggaaaatgct cgggccgagc tgttggtagt
     5941 cctgttcgaa gcaccagcgc tgcgccccct cgacctcgtc ggtggtcatg gcggggtgct
     6001 tgaccatgac ggagaagccg tcggcttttc ggaaatattt ttccggctgg cctttgaagc
     6061 agtcccggaa cctgttttct ttttccatcc gggcgtagag cggcgttccc ggcacggggc
     6121 cgtaaatcag gaattgagac agggcggggt ggagtttcat aaggccggcg tgttcctcgg
     6181 cgatgatgtc gcggttttgg tattcgaacc ccacgatcat ggacgccagg atggtgatgc
     6241 cgtgttcgcg gaattcggtg aagatgtccg ccaccgggcg accctgttgc ttggcgtagc
     6301 cggaccgggt cccttcgtag cccacccaga tgccgtcaat gcccatttca aggatctgtt
     6361 ccaccgtgta ctggctgatg gctttcacgc tcgaaaaaac gaaaatggac gggacccgcc
     6421 cccccttgct gacgcagtcg cggaattcca tggcgcgggt tttgttcagg aggaaatcct
     6481 catcaaaaag catgaacacc gtctggggat cgttcttcca atagcgctcg atcacatcgt
     6541 agatgtcttt cccggtgggg aggagtcgga tgtgtttccg gccgaaatag tgggaggtgc
     6601 agcagaaatc gcagccgttg gggcaaccga gcccggcgaa gatctggccc gtgcgggaga
     6661 tcgggatgga aaaaagtttg agggtgctca gcatgatcgg gtgctcgtag ggcatggaca
     6721 gcggcgtttc gcccagcagt tcgcggaaaa atccgacgcc ctctccccgg caaacgaaat
     6781 cgccgtaggg tttcagcagg tcgtcctcca ggatggtgcc gtatccgccc aggacgatct
     6841 tggtttttgg ggaatgtttc cggatgagct ccacggtgtc cttcattttg tggaacaccg
     6901 ccatgatgaa ggagacgccg acgtagtcat agcctttttc aagttcccga atgagctccg
     6961 cgcgcgaagg gtactgcagg accaccgtgg gggtctcgag gttggcggcg atgtagtcga
     7021 gggagaaaaa cgagttgatg acccgcgggc tgaaaattcc ctgggcccgg gtcacctgtt
     7081 tgtggaggag ttcgtaaccc acggaggggg cgtccccggc gctttcgccg aaaggccggc
     7141 agacgctggt cagaagcact tttttcgtcg tcaaattggt cctcatttcc ttcggaaatt
     7201 cgaaaaaatc aagaggccat cgcctgaaaa gatgactttg tttttatcgg cgcgcgtcgg
     7261 gcacggagat ttcggtgttt ttcagcaaac tggccagtgg gacacgaatg ggggagcgct
     7321 cttatccatc ggctggtcaa ccaccgtcac tttaacataa taggacgtgt ttcgggggaa
     7381 ataggtgcgc gggggcggcg gggggcggga ttcaattttt aaggcgtccg cgcttccgcc
     7441 gcatgatgtc cacgagggtg tcgtccacct gggccatgcc gacagtagcg attttcgcca
     7501 gattgcggat ggtttcttcc gcggtgtgcc cgacgaaccc ctcgatggcg gtaatgccgt
     7561 aatgccgggc ggccatgtag gcggagcgga tcgcgcagtc ggcggagctg accactttca
     7621 gggcgcagcc gctcttggcg ccgtcgcaga gcatccctcc gatgtcgctg acgacattat
     7681 tgatggcgag tgtgatggcc ttgaggtccc cccccttctg ttgatacgcg acggcggcgg
     7741 cggcgcctac cccggcggcg atggcgcagc cgcagagggg cgagagttcg ccgatctggg
     7801 ccttgacata ggcgttgacg agatgggaaa gggcgatgct tttcaatatg cgccggcggg
     7861 agattttaaa agcttgtccc acgttgtggg gaacaaggac ggcgacgatc ccctggttcc
     7921 cgctctcgcc gctgcacatc accgggaccg ggcagcccgc cattcgggca tccaccgcgc
     7981 acgaaacaat aattttcgtc gacgagaaga cgtcgttgag gaggtatccg cgttttcgga
     8041 ggtctttaag ataaaacccc acttttttga gcgccattcc ggcctcggcc gcttttagat
     8101 tcgttgtgac cccctcctcg atgtaggccg cgtcggcgtt gtccatctgt tctgccgcgc
     8161 ggaggagtcc gcgcagggag atttttttca ggattttttt aaagtgaggg gaggcgcttc
     8221 tccccgagac cgagcggtcg atgagggtgg tgtcgtcgcg cagaaggagg gaggtctcat
     8281 tatgatggtc tcggacgatg caggtggcgc ggtggcggcg tccttccacg cggacctcga
     8341 tatggatgcc tttcttccga gggagaaccg tgaacgaaac gcgcccttgt cggatcgccc
     8401 gcgcggcttt ttgggccagg gacggcgtgc aggtcttgag aagctgcatc tttaaatggg
     8461 gtgctgcgag gaacagcccc attgtgcccg ccaggagaca cccccgtcct ccggcggtgt
     8521 tggggaggag cacaccgacg ccgtttttgt aggtcgctct gtccgtgaag atttcggccc
     8581 ggcggaccgg ttctcccaag gcgtgggcgg cccgggaagc gcagagggcc accgcccccg
     8641 gttccgtgca gccgatggcg ggataaacct gggcgctcag cacctcctta agaagattca
     8701 tcccggcaac ggcattcctt tcttggcggc cacgggacgg gcgtcattcg ccgtgatgat
     8761 gggcgcacgc catatcgggg gtcacttcgg ggagctgtcc ttggttgtag gcgcttacgg
     8821 tttccttgag ggagggaaag gccgttttgt aaaccgcgat gccggcggag cgaagtctgt
     8881 tcagcgctcc cgcgccgatc ccgccgacga cgatcccgtc aaatttttct ttaccgagga
     8941 ccgccagagg ctgacacatc ccgtgggcat ggtgttgatt ggcgttgggg atggttttga
     9001 gggacagggt ttcggtatcc acgatcaaaa aaaacggcgc ggagccaaaa tgagcgcaga
     9061 tgggactgga gaatcctgcg tttttcaata cgggcagaca gagtttcatg gggcatctcc
     9121 ttttaggggt atttcgattt taagcgcttt tccgcccagg agggcgtcgg cgattttttt
     9181 atgggcggaa tcgatgatgc gtccgaaggt ttgccgggag atgcgcattt gtccggcggc
     9241 ctcttcttga tagaggccgt tcaggtcggc caggcgcacg gcctccaatt cgtcggggcg
     9301 aaggacggtt tcctcaagtt cgacgagggg gatgccccgc ggcttgaaat aattcgagtt
     9361 cacgaggcag ccgatgcggc ggcatttccg ggggcgcatg ccttattatg agcatatgcc
     9421 cataataatg tcaacttctc gggcggtggg tggcgaacgg cgtggggttt gtttgtgtta
     9481 tcggcgggag agggcgcgca ggcggaaggc cacggccacc gcacccgcca ccagcacgcc
     9541 gtagaggatt ttcccccaaa gatcgtcgag gaggcggatg agcggcggca tcaggacgag
     9601 gaagaggatg atgtagagaa gcgcgaaaat gatttcctga aaggcttttc tagtttgttg
     9661 ttccaaggtg cgtctccttc agccggacga tctctttcat cacccggtcc gaaaaggcct
     9721 gatattcttt ccccccgccg gcggacaggg gggcgccgaa acagacccac agccgaggca
     9781 gggaccagag ccggtaggtg ttgtgcacgt acaccgggac caccggggcg ttcgtcttgg
     9841 cggccagcat gccgaccccg gccttggcgg ccgccagttt gccggtccgc tgccgttttc
     9901 cttccggaaa gacgatcatg gccttgcccg agatgatgag ccgctgagcg gtcttgaagg
     9961 cgccgacgtc gtgttccgtg cgtttcaccg ggaaggcgtt cacccggcgg atgattccgc
    10021 cgaaaagagg gacatcgaac agttcctgtt tgcccatgaa atagatcggc cgcggcaggc
    10081 tgcagccgat gacgggcggg tccgcccagg aggcgtggtt ggcggccagg atcaatgggc
    10141 cttcgagcgg gatgttttcc cggccgacga tcttcagtcg ccagacgagc ttatagacgg
    10201 tgaaaaaaaa ggtatggccg agccagtaaa ggaaccggtt cagccggggt tcggggtggg
    10261 gatgcgggcg gggcggcgtt tctttggccg tttccatgat ggcgccgggc tcagtcattc
    10321 ccgttgcggc cttcgcggcg gacgtggttg aggatgaact tgaaaacgtc gtggagcgtc
    10381 atcttcgtgg tgtcgatgac ggtggcgccc ggggcgacct tgagcggcga gatgccgcgc
    10441 gtccggtcct tgtagtctcg ttggcggatg gcctcggcga tggaatccag gctgacgcgt
    10501 ttgttcttcg ctttcagctc gcgatatcga cggagcgcgc gttccatggg ggaggcgtcg
    10561 agatagaact tgaagtccgc ctgggggaac accgcggtgc cgatgtcgcg cccttccata
    10621 acgacgccgc cttcgcggcc catttcgcgc tggcgttggc gcaggacctg gcgcaccccc
    10681 ttgaccgcgg cgagggtgtt ggtcagtcgg gtgacgcgtt gggtgcggat gcgtccggtg
    10741 acgtcttccc cgtccgccag catgcgggag acgccttttt tctgcacgaa gcggagtttt
    10801 gtttttttcg ccagggagga gagcgcttcc ggcgagtgga aggggacccg gtcgcgcagg
    10861 gccttccagg tgagcgcgcg gtacatggcg cccgtgtcca cgtagcggta gcccaattcc
    10921 tgggacagaa gtttggcgat ggtggatttt cccgctcccg cgggaccgtc gatggcgatg
    10981 acgctttttc tcggcatggg gggccgcctc ctaaattttt taagtcgtgg cgacgcgaag
    11041 tttttcaaat tcttcccaga acgtcgggaa ggagatgtcc acgcagtcgg cgttgcggat
    11101 gctggtgttg ccgtcggcga tgaggccggc cacggcgaag gccatggcca tgcgatggtc
    11161 gttgaaagag tccacctcgg cgccccggag gggcgtgggg ccgcggatga tgagcccatc
    11221 ggggagttcc tgaatgcggg cgcccatctt ggtgagttcc gacgtgacgt gggccaggcg
    11281 gtcggactct ttgacgcgga gttcggcggc tccgcggatc tcggtgacgc cttcggcctg
    11341 cgtggcggcc acggtgagga tggggatctc gtccagcagg cgcgggatga tctcctcgtc
    11401 cacccgggtc gccttgagcc cggaagtctt taccaccagg tcgcagacgg gttctccccc
    11461 catcacccgg atgtcctccc gtttgatgtt ggcgcccatc gcctgcagga cctccaggag
    11521 gccgtcccgc gtggggttca cgtccacgtt ccgcaggagg atttccgagc cgggtgcgat
    11581 gagggccgcc accaggggga acgcggcgct ggagatgtcg ctgggaacca cccattcctg
    11641 ggcggtgagc gtttggccgc cccggacggc gacgcggttg ccctggatgt ccagcttgat
    11701 gccggcggcg gagaacatcc gttcggtgtg gtcccgggac ttgaaggttt cctccacgga
    11761 ggtgatgcct tcggtgtaaa gtccggcgag caacacgcag gacttgacct gtgcgctcgg
    11821 cacgggggac ttccaatcga cgggcgtcag cggccggcgg ccgtggatgt ggagcggcgc
    11881 gtatttgccg tcgcgggcgg cgatctccgc acccatggcg cggagcggct gggccacgcg
    11941 gtccatgggc cggttggaaa gactgtggtc tcccatgagt ttggtctcga acggctgccc
    12001 ggccagcacg ccggaaacga gccgcatggt ggtgccggag ttgccgcagt aaatcgtctg
    12061 cgagggggcc tcgttcaggc cgtagagtcc ccggccgacc acgacccagg ccccggtgcg
    12121 gtcccgctcc acgctgatgc cgagggaacg gaaggcctct gcggtgcgtt cgcagtcgtc
    12181 ggcggccagc gggtggatga tgcgcgaggt gccggtggag agggccgaga ggatgatggc
    12241 ccggtgcgtg atggatttgt ccggcggggg gagggtttcg ccgcgaaggc tgccgccggg
    12301 gacgacgatg cgcgaggagg tggaaacggt catctcagtg gtccgctaaa agagagtgca
    12361 gccgcccggc ttttgctagg gggatccggc ggttccgggc gccgacgagt cggcgcagga
    12421 ttttttggaa ccgcgcggcg gcgactttca ggtttttgtc gttcatgcgg aaaatggcgt
    12481 cccattggcg tggatcggcc tcggcgaccc gcgtcatgtc gcggaaactt cccgcggcca
    12541 ggagacggag tgtccgtcgg gccgaagggc cccggtccgc ggccaggacc agacagtccg
    12601 ccagcaggtg cggcaggtgg ctcaccagag ccatccagcg gtcgtgcgtg accgcgtcca
    12661 gccgcaccac ccgggccccg gccccgcgcc agaaagaccg ggcgcgttcc agcgccgcgc
    12721 ggggcgtgtt tcccgacggc gtcagcacgc agacggcgtc ctgatagagg tcggcgcggg
    12781 cgtgctggac gcccgttttt tccgaaccgg ccatggggtg ggcgcccacg aagtgggggc
    12841 cgcccgagga tgaaaaaatc ttttgcaggg aacggacgat gggcgccttg acgctgccga
    12901 cgtccatcac caaggccccg gttttcaggt gggggcggat ccggcgggcg aggggggcga
    12961 tttcgtccac gggcacggcg aggaccacga tgtccgcgtc ccggacgccc gcggccaggt
    13021 ccgtggtgtg ttcctgcacg gccccgtgcc gccgcgccag gcgcagacgc tgcgcgttcc
    13081 gtcccacgcc cgccacgtgc cagcgacgtt tcttccggag gagcgccagt cccagcgccc
    13141 cgcccatgag acccactccg atgatggcca cgcgttccgg tttcatttgg gagagttccc
    13201 cgcctccggc gttaaagttc ccggccgacc gcggccgcga tgccgcggag ttccttcatg
    13261 agctgcgcca tgtggaccgg caacagggcc tggtgcgcgt ccgacagggc tgtggcgggg
    13321 ttgggatgca cctcgacgat gatgccgtcg gccccggccg cgatggccgc gcgcgccatg
    13381 ggcgcgatgt agtcccgcac ccccacgccg tgcgacgggt ccacgaacac cggcagatgg
    13441 ctgaggtgct tgaggacggg gacggcgttg aggtcgaggg tgaaacgggt ggcggtctcg
    13501 aaagtgcgga tgccgcgttc gcagaggatg acgttggggt tgcccttggc gaccacatat
    13561 tccgcggaca tcagccattc ctcgacggtg gtggccatgc cgcgcttgag gagcacgggc
    13621 ttgcgggagc ttcccagttc cttcaacagc tcgaagtttt gggcgttgcg cgcgccgact
    13681 tgatagatgt cggcgtagcg ttccaggagt tccacctgcc gggtgtccat cacttcggtg
    13741 acgaacggca tcccggtggc tttgcgcgcc tcggccaggt aacgcagggc ctcttcgccc
    13801 agcccctgaa aggcgtacgg ggaggtgcgc ggcttgaagg cgcctccgcg aaggatgtgg
    13861 gcccccatct ttttgagttc tttggccacg cgcaggagat tgtcgcgggt gtcgacggaa
    13921 caggggccgg ccatgacgat gatgtttttc ccgccgatgg aaaatccgtc gaagtcgatc
    13981 cgcgtgtctg tctttttgaa ttcgcggctc accatcttgt agggcttgga gatgggggtg
    14041 acggtctcga tctgctccat ggactcgaag acgtttcggt aggcgatggc acggttgccc
    14101 tcgccgatga cgccgatgac gatcttctcc gtccccttgg agagttccgg ggtgaagccg
    14161 agttccttga tcttggcgag gacctcgtcg atctccgttt ttttgacgcc ggttttgagt
    14221 gtgacgatca tgggctttgt cggacctcct ggaacgcttt gagaaagagg gtgttttctt
    14281 ccggccggcc gacggtgacg cggagatagt gcggcaggcc gtattccgcc acggcgcgca
    14341 cgatgacgcc ctttttgaga agtgccgcaa aaacgtccgc ccccttgtgc ggggagacgt
    14401 cgatgagcag gaagttcccg gcggagggga cataggcgac gtccatcttt cgaaacgccg
    14461 cctcaagccg ggccatctcg gttttgacca ggcgcaggga gcggcgcacc tggtttttgt
    14521 cggcgagcgc cgcggcggcg gcgacctgcg cggcgtagga gatgttgaac ggcggg
//
LOCUS       DAOTFD010000046        14379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_2913_length_14379_cov_4.277018, whole genome shotgun sequence.
ACCESSION   DAOTFD010000046 DAOTFD010000000
VERSION     DAOTFD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2913_length_14379_cov_4.277018"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="P5079_04470"
     CDS             <1..125
                     /locus_tag="P5079_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HRY29275.1"
                     /translation="RETIYTTLCRIREDMALECHIDTDEANAAGVKNGDSAVML"
     gene            244..543
                     /gene="eutM"
                     /locus_tag="P5079_04475"
     CDS             244..543
                     /gene="eutM"
                     /locus_tag="P5079_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HRY29276.1"
                     /translation="MEALGMIETRGLVSLVEAADAAVKAANVKLVGWEKIGSGLVTVL
                     FRGEVAACRSACDAGAASAQKVGELVAVHVIPQPHFDLEGKLPISLPETLGRGRK"
     gene            595..903
                     /locus_tag="P5079_04480"
     CDS             595..903
                     /locus_tag="P5079_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY29277.1"
                     /translation="MIFARVIGTVVCTRKDEKLVGTKLLLVQPVDLKGQAKGNPLVSI
                     DAVGAGEGEVVLVVQGSSARQTRMTEGNPVDCTIFAVVDSVEQAGKVLFRKATDFAEA
                     "
     gene            975..2432
                     /locus_tag="P5079_04485"
     CDS             975..2432
                     /locus_tag="P5079_04485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRY29278.1"
                     /translation="MEFKEQEIQSLVQEILKRLETGSVRPGAGPSAPSAPAAGTNPCV
                     FSSIDDAVSAAEKAQAVFQDMGMDFRRKLIEAMREAARRNAEKLSRLACEETGMGRVE
                     DKIVKNVLIADKTPGVEDLQPVSAFTGDNGLTLVEYAPFGVVAAVAPSTNPSSTIVNN
                     SICILSGGNSVVFAPHPAAKKCCQETMRLLDEAIRSVGGPAGLITTFEPISQEGTKAL
                     LSHPRIRCSVVTGGPAIVKVAMGAGKPQKLICAGPGNPPVIVDEYADFSRCARDIISG
                     ASFDNCVLCTGEKEVIVTAAAWDKFLPAMRQDPRAHELTTAQMDELCKKIILESGKGG
                     REPKVNRDYIGKNANVIAQKGLGLNLPESVRLLWGVVPNDHDFIWTEQLMPLLPVTQA
                     RDFDAALSLAKQAEGGNNHTASIYSLHVGNLTRAARTMACSIFVKNGSNFMGLGRGEG
                     YATVSIGTPTGDGLTKPRHFVRPLHCSLVGYFRIA"
     gene            2450..3019
                     /locus_tag="P5079_04490"
     CDS             2450..3019
                     /locus_tag="P5079_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY29279.1"
                     /translation="MNGNDLSNKNPSIGFIEVSSIAKGIEASDAMMKMAEVELLMTAV
                     LARGKYVVMIGGLLADVQQSLKAGLDIAGSSVVDHFVIQNVHPQLPAAIKARVKVDDI
                     EAVGVIETKEVASAIYAADAAVKAASVKLIEARPQSGGKGLAVFTGEVGSVRSAMSAG
                     LATVKKDGMLISHILLPYAHKALLKSLTA"
     gene            3038..3496
                     /locus_tag="P5079_04495"
     CDS             3038..3496
                     /locus_tag="P5079_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY29280.1"
                     /translation="MNLVIGSDHGGFEAKEKIKKFLLAQGHSVTDFGCYSLEAVDYPD
                     IALLVGEAVIRREYERGILLDGTGGGMTLAANKVPGVRAVCAYSELTGKYASEHDDAN
                     VLCLGGKTVGELILQETVRAWVETPFGGGRHTRRLGKIAEIEKKYSRMPF"
     gene            3497..3949
                     /gene="rpiB"
                     /locus_tag="P5079_04500"
     CDS             3497..3949
                     /gene="rpiB"
                     /locus_tag="P5079_04500"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRY29281.1"
                     /translation="MKVAIGFDHAGVRHKSAIAGYLRARGHRVLDKGTTGDDAVDYPD
                     YARAVAESVRRGESDRGVLVCGSGVGMCIAANKVKGVRAALVWTPKVARLAAEHNHAN
                     VLCLAGRFFTSKEIVRMLGAWLGASYVPGRHARRVKKISAMEKGECGC"
     gene            3987..4328
                     /locus_tag="P5079_04505"
     CDS             3987..4328
                     /locus_tag="P5079_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY29282.1"
                     /translation="MKAAQVIGRVVATRKYETLEGKKLLLLQPMTWEKEPVGDPIVAV
                     DVCGVGADEFVFFVAAREAAAAFPDLPPVDAAVVGIIDGVNLQVWTMPEGTPLRAAHA
                     KSEIRKTKENE"
     gene            4377..4673
                     /locus_tag="P5079_04510"
     CDS             4377..4673
                     /locus_tag="P5079_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY29283.1"
                     /translation="MFIAKVIGSVWCTKQHDALNRKKLMLVQPVDFMGGRAIGDPVMA
                     VDVKMGAGPGDTVLVMDEGNSARIILQDKKAPVRTVICGVVDSVTVRGKTYKFH"
     gene            5594..6253
                     /gene="deoC"
                     /locus_tag="P5079_04515"
     CDS             5594..6253
                     /gene="deoC"
                     /locus_tag="P5079_04515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRY29284.1"
                     /translation="MAFLIDHTLLKPNATQEEVGKLCEEAKKYCFASVCINPAYVPLA
                     AQLLRGSGVRVCTVIGFPLGSTTPTVKAIEARDAIANGADEIDMVINVGAVKSGNYAL
                     VLDDIRAVRDATRGKVLKVIIETAYLTREEKVKACELSKEAGADFVKTSTGFGPGGAT
                     VEDIALMRSVVGPTMGVKASGGIRDQETALKMVEAGATRIGASASVSITMGKKAGEGK
                     Y"
     gene            6306..6590
                     /locus_tag="P5079_04520"
     CDS             6306..6590
                     /locus_tag="P5079_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY29285.1"
                     /translation="MTEALGMIETRGLVGMIEASDAMAKAAKVKLVGFEKIGSGLVTT
                     LCRGEVGAVRAAVEAGAAAAQKVGELVAVHVIPRPHNDLEKYVRKVEGSF"
     gene            6621..6932
                     /locus_tag="P5079_04525"
     CDS             6621..6932
                     /locus_tag="P5079_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29286.1"
                     /translation="MLTEDDLIDIIADALARRLGPAEWRDGRSVSPSRQGKWGFHVQK
                     PFNRPWTALPMKRPFITEYMARQMAGPGREIRIPRSAILSPLAEDWLMAHGVAVVRAE
                     Q"
     gene            6946..8040
                     /locus_tag="P5079_04530"
     CDS             6946..8040
                     /locus_tag="P5079_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY29287.1"
                     /translation="MAIDVTQLFKTMVRTGISDIHFKAGTPPMVRVHGRMMSSGFTKM
                     AAQHIEELADVLMNTEQKAMFARDKELDISFEVPNVSRFRVNVYRQKGTMALSLRVVP
                     LTLRTLAELNLPEETLKKLCANTRGLILVAGITGAGKTTTLNALIDHINKTYAYNIVT
                     VEDPIEYYHTDAKSSVAQREVGGDTESFLRAMKHVLRQDPDVVVLGEMRDADSIRAGV
                     MAAETGHLVLGTIHTMNAAQTLDRLLEAYDVSEQMGARQRTANILRGIICQRLLETAD
                     GNGRVPATEIMVVTSLIQKLMLEGKTAELRKAIEQGNYYGMHSFDQDLLRLVNEKKIS
                     KEEALDASNNPDDLMIKLNTQGLSGGAGPA"
     gene            8072..9112
                     /locus_tag="P5079_04535"
     CDS             8072..9112
                     /locus_tag="P5079_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29288.1"
                     /translation="MSIERWELVPSSRADRYLLRVRGDRAEVDAIVRAYSRVCEEPRE
                     ISDPVFQWAIYVVDATLNERLAIQDQLRNRMTRSTEPSLVAPVDLSGVLAELSLVLEE
                     LTGLTDEEQAHVAKKMQEIQQREAEQAAAPLPPPPVPPQEPERKIVLDVAPRTPPVRE
                     PAPPKAAPVPPPPSPEPPAVPKPSAAPSLPPSSPPPASPPPVEAASVSAPLPPAPPSP
                     DVPPDQLLRAVFFYAEGAENQKNKFVEKLTDVAQKKAKKPLFIHPVASRATALVAAKA
                     GEWIQTSRSSGADCFFVLLTPDVLPDFLERAANEARQSGLHCFLVPQAELESRLLYVD
                     LMVELMLIKRKR"
     gene            9134..10183
                     /locus_tag="P5079_04540"
     CDS             9134..10183
                     /locus_tag="P5079_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29289.1"
                     /translation="MIERWEIVADPHERFPNRHRVSVAGEESDVQSLLAAMARKAGKP
                     WALRRGAFRFAFYLYDPTEEDRRRVEKFLAGDRPTVSGKTAVSPEGRSALPPESSPVK
                     PFSWEEAGAEVEVLSDLPPAAGKPSREETKPSAGSSDWERPSLVESATDALMSWAETG
                     PTERKPSSVPPASPAEPPPPTIQVERTEKKEEEEDGEDSPPSGLIVATEETGMPMEEM
                     VVSSAPEPEVPKGYLMFRLGYFIPHDIGGVADRIHSLFSQTLETRHMPFVFHKVFTFP
                     YRWPDRPLVDQVIAGCQKHKIDGLVCVGDEKRLELLFEQCQEKGIKHHFLRREDTQKK
                     YWRLGLITRIVVTDY"
     gene            10256..11647
                     /locus_tag="P5079_04545"
     CDS             10256..11647
                     /locus_tag="P5079_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY29290.1"
                     /translation="MTEPRGLPETRLNPRYTFEEFVVGPHSRFPHAAAQAVSENLGKA
                     YNPLFIYGPVGVGKTHLMQAVGHRVLGRDGQTHVLYITAEEFMTDVIDLLRSGNLKSF
                     RDNFRSLDLLMVDDVQFLSASESTQEEFFHTFNALHQAGKQIIMTSDRPPKMLTTLED
                     RLRSRFEWGLIADIKSPSLETRVAILRKKESQFGNMQLGEDIRLYIAGKLKSNVRELE
                     GFLRRVQAYAQLNNQTINLPVVKEIMRELLPPSSWDEEDQAALAETQHPGHTPPAGGG
                     KAGVAPVLLPPVSAPPAAAGESAHRVTPAISPDAIKIDSEISIVLPPAVEPRVETGPK
                     SVVAAFFYPAGKERELAQLKQKFEDVIHKHKLKFQLDAATEAPYPLEETLSYDTFVQA
                     CAQKSCHIAIVLGPPPGSRLKDFDFHQKLQELFDSHRVSLQLIPWEELGKDYRFLNIA
                     LDITLIRYKEKAP"
     gene            11644..>14379
                     /locus_tag="P5079_04550"
     CDS             11644..>14379
                     /locus_tag="P5079_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY29291.1"
                     /translation="MIRPRFSVGFQLALLACVSVAAAVGLWAGLSTVAVKRYVREEVS
                     RRAFQYVGEVSRLAGRTAQGRSEKWVEMTPLLDRLVGEPGVRYLIIYGPQKKGILAQP
                     SVPSPLNGTEESFTMPVMDAAGRVIASARLWYSPEAYLGTLWEKGRGPLFLLALLTGV
                     GLGGVIYAGCWWLVFRPIRKLLDRSENAFLPGKAAAPLDKNEFDAFLRRFHELRKKLA
                     ESEENFSVLLSASKTMTSDMDIQEIHNRILDLVWRRLDRDPCLLWWQDEDEFLRIRNF
                     RNFPPELIKRLRLKPGESGVGQCLVARTPGVETSLASTSEKWLEELHVSLDINSAAHF
                     PLLVDGKSLGVLSAFSRHGDFFSEERMRMIASLMEYLSLATKSARLFEEMQTFNRRMK
                     SEIASTTRELAQTNVRLIRRVRELRALYDLAAQPESPSTEHMFSRTLEHLVDLLEVRR
                     SAVFLWDEHAQRYRSAVAFLSENAVSVESLTPWISRLQAGDPVFLERAEDVRTLFPDS
                     AAVSLLMLSPIRAEKSLLGFIAAADKRGGRFDEEDVRLLALFARHIADILQGVQFRRE
                     REQRLRDLTLLQEVGAVLSSEPDLEQTLRKTSKVVAESLGADICAFLLYDVQSDELVT
                     QPGAYGLSEDADASIVRIRLDDPKSANVRVFRTGKALLSRDVEADPEVRPHYATLWNI
                     RSLMLIPLIAEQKTIGVLRLGHKSPNVFTEDHLRLARLVAEQAAVIVQNAKLYRQIRE
                     NVTELRRLNNIKSEFLSMVSHELKTPLTTLKGYVGVLLNEKNGPLTAQQQRFLNLAKL
                     ALNRFSMLIYDLVDSSALEAGKLKIEPAPTVLGELLQQAYQDHLPAAQLRKINLVLEP
                     LPDALPVEADASRIRQVVDNLVTNAFKFTSEGGAVTLSLEEVPEGMRVSVRDSGIG"
BASE COUNT         2700 a         4436 c         4679 g         2564 t
ORIGIN      
        1 cccgggagac gatctacacc accctctgcc gcatccggga ggacatggcc ctggaatgtc
       61 acatcgatac cgacgaggcc aacgcggccg gggtgaagaa cggcgattcc gcggtgatgc
      121 tgtagggccg cggggtcgcg tcactttttt caagcggttg aagggaacga tatcgtcgga
      181 aacgccgacc gcccctcgcg ggcggaacga agttttccgg aaaacgaaac aaggaggaca
      241 taaatggaag cacttggcat gattgaaacg cgcggcttgg tgtcgctggt ggaagcggcg
      301 gacgccgcgg tgaaggccgc caacgtgaag ctggtgggtt gggagaagat cggcagcggt
      361 ttggtgacgg tgttgttccg cggcgaagtg gcggcgtgcc gttccgcctg cgacgccggc
      421 gccgcctcgg cccagaaggt gggcgaactg gtggcggtgc acgtcatccc gcagcctcat
      481 ttcgacctgg aaggcaagct gccgatttct cttccggaga cgctgggccg gggacggaaa
      541 taacacgacc gacgacgccc cgttttttga ccggggcgga atgagggcac acgcatgata
      601 ttcgctcgag tgatcgggac ggtggtctgc acgcgcaaag acgagaaact cgtcggcacc
      661 aagcttctcc tcgttcagcc cgtggatctg aaagggcagg cgaaagggaa cccgctggtg
      721 tccatcgacg cggtgggcgc cggcgaaggg gaagtggtcc tcgtggtcca gggttccagc
      781 gcccgccaga cgcgcatgac cgagggcaac cccgtggact gcaccatctt cgccgtggtg
      841 gattccgtcg agcaggccgg caaggtcctg ttccggaaag ccaccgactt cgccgaagcc
      901 tgacgtcatc caacaacttg ctgccgaacg accccctttg accggggcgg ccgtccgctt
      961 tttttggagg ttctatggag tttaaagaac aggaaatcca atcgttggtg caggagatat
     1021 tgaaacgcct ggagacgggg tccgtccgtc ccggcgccgg tccgtcggcg ccctcggccc
     1081 cggcggcggg gacgaacccc tgcgtgtttt cgtccatcga cgacgcggtg tccgcggcgg
     1141 aaaaagccca ggcggtgttc caggacatgg gcatggactt ccgccggaag ctgatcgagg
     1201 ccatgcgtga agcggcccgc cgtaacgcgg agaaactctc ccgcctggcg tgcgaggaga
     1261 ccggcatggg ccgcgtcgag gataaaatcg tcaagaacgt cctcatcgcc gacaagacgc
     1321 ccggcgtgga ggacctccag cccgtgtcgg ccttcaccgg cgacaacggc ctcacgctcg
     1381 tcgagtacgc tccgttcggc gtggtggccg cggtggcgcc gtccaccaac ccctcctcca
     1441 ccatcgtcaa taactccatc tgcatccttt ccggcggaaa ctcggtcgtc ttcgctccgc
     1501 acccggcggc caagaaatgc tgtcaggaga ccatgcggct tctggacgag gccatccgat
     1561 ccgtcggcgg cccggccgga ctcatcacca ccttcgagcc catctcgcag gagggaacca
     1621 aggccctcct ctcccatccg cgcatccgct gcagcgtggt gacgggcggc ccggccatcg
     1681 tgaaagtggc catgggcgcg ggcaaaccgc agaaactcat ctgcgccggc cccggcaacc
     1741 cgccggtcat cgtggacgaa tacgcggatt tttcccggtg cgccagggac atcatctccg
     1801 gcgccagttt cgacaattgc gtcctctgca ccggcgaaaa agaggtcatc gtcaccgcgg
     1861 cggcgtggga caagttcctt cccgccatgc gccaggaccc ccgcgcccac gaactcacca
     1921 ccgcccagat ggacgagttg tgcaaaaaga tcatcctcga gagcggcaaa ggcggccgcg
     1981 agcccaaggt gaaccgcgat tacatcggca agaacgccaa tgtcatcgcg cagaaggggc
     2041 tgggactcaa tctgccggaa tccgtccgtc tgttgtgggg ggtcgtcccc aacgaccacg
     2101 atttcatctg gacggaacag ctgatgcctt tgttgcccgt cacccaggcg cgggatttcg
     2161 acgccgcgct gtccctggcc aaacaggccg aaggcggcaa taaccacacc gcctccattt
     2221 attcgctgca tgtgggcaac ctcacgcggg cggcccgcac catggcctgc tccatcttcg
     2281 tcaagaacgg ctccaacttc atgggcctgg gccgggggga aggatacgcc accgtgtcca
     2341 tcggcacgcc caccggcgac gggcttacca agccgcgcca tttcgtgcgg ccgctccact
     2401 gcagcctcgt cggttatttc aggattgctt aatcaaggag aattgaccta tgaacggaaa
     2461 cgatttatcg aacaaaaatc cttccatcgg atttatcgag gtaagttcca tcgccaaggg
     2521 catcgaggcg tccgacgcca tgatgaagat ggccgaggtg gaactgctca tgaccgcggt
     2581 gctggcgcgg ggaaaatacg tggtgatgat cggcggcctc ctggccgacg tgcaacagtc
     2641 cctgaaggcc ggactggaca tcgccggctc ctcggtggtg gaccatttcg tcatccagaa
     2701 cgtgcatccg cagctgcccg cggccatcaa ggcccgggtg aaggtggacg acatcgaggc
     2761 ggtgggcgtc attgagacca aagaagtggc ctcggccatc tacgccgccg acgcggcggt
     2821 gaaggccgcc tcagtgaagc tgatcgaggc gcggcctcag tccggcggga aagggctggc
     2881 ggtgtttacc ggcgaggtgg gctcggtccg ttcggccatg tccgccgggc tggccaccgt
     2941 caaaaaggac gggatgctga tatcccacat cctccttccc tacgcccaca aagcgctctt
     3001 gaagagtctg acggcgtaaa gagatttttt catctttatg aaccttgtca tcggttccga
     3061 tcacggcggt ttcgaggcga aggaaaaaat aaaaaagttc cttcttgccc aggggcattc
     3121 cgtcacggat ttcgggtgct actcgctgga ggcggtggac tacccggaca tcgcccttct
     3181 ggtgggcgag gcggtgatcc gccgggaata cgagcgcggc atcctgttgg acggcaccgg
     3241 cggcggcatg acgttggcgg ccaacaaggt gccgggggtg cgcgccgtct gcgcctacag
     3301 cgaactcacc ggcaaatacg cctcggaaca cgacgacgcc aacgtgttgt gcctgggcgg
     3361 aaaaaccgtg ggggaattga ttttgcagga gaccgtgagg gcctgggtgg aaacgccctt
     3421 cggcggcggc cgtcacacgc gtcggctggg gaagatcgcc gaaatcgaaa aaaaatattc
     3481 acggatgccg ttctaaatga aagtagccat cggattcgac cacgcggggg tgcggcataa
     3541 gtccgccatc gccggctatc tccgggcccg gggccaccgg gtgctcgata agggcacgac
     3601 gggggacgac gcggtggatt atccggacta cgcccgcgcc gtggccgaat cggtgcggcg
     3661 gggggaaagc gatcggggcg tgctggtgtg cggcagcggc gtgggcatgt gcatcgccgc
     3721 caataaggtg aaaggcgtgc gcgcggcgct ggtgtggacg ccgaaggtcg cccgcctggc
     3781 ggcggaacat aaccacgcca acgtgttgtg cctggccggc cggtttttta cttcgaagga
     3841 gatcgtccgg atgctcggcg cctggttggg ggcgtcctac gttccgggcc gccatgcccg
     3901 gcgggtcaaa aagatttcgg ccatggaaaa aggggaatgc ggatgctgat cgttacgcgg
     3961 tttaagcctc aagcgggcgg tcgcccgtga aagccgccca ggtcatcggc cgcgtggtgg
     4021 ccacgcgcaa atacgagacg ctcgagggga aaaagctcct gctcctccag cccatgacct
     4081 gggagaagga gccggtggga gaccccatcg tggcggtgga cgtgtgcggc gttggcgcgg
     4141 acgaatttgt tttttttgtg gccgcccggg aagccgcggc ggcgtttccc gacctgccgc
     4201 ccgtggacgc ggcggtggtg ggcatcatcg acggggtgaa cctgcaggtc tggaccatgc
     4261 cggaaggcac gccgctgaga gcggcacatg cgaaatctga aatacgaaag acgaaagaga
     4321 atgaataacg ctggccgttc ttcgcatttc gtatttcgta tctcgtatct ctgactatgt
     4381 ttattgccaa agtgatcggc agcgtctggt gcaccaaaca gcacgacgcg ttgaaccgca
     4441 agaaactgat gctggttcag ccggtggatt tcatgggagg cagggcgatc ggcgatccgg
     4501 tgatggcggt ggacgtcaaa atgggggccg gcccgggcga cacggtgctg gtgatggacg
     4561 agggaaactc ggcccggatc attttgcagg acaaaaaggc ccccgtgcgc accgttattt
     4621 gcggggtggt ggattccgtg accgtgcggg gcaagacgta caaatttcat tgatcgaata
     4681 aatcatcgag gaaagaaaaa tggctgacat ggacagaaaa gacgtagaaa agatagttcg
     4741 ggacgttttg accgattaca tggcttcgcg gagggcgtcg tctcccctct ctccggcgcc
     4801 ggcgccggcc gtgcggtccg gcggttcggg aggcccgcgc gtgctggcgg tggtgatggc
     4861 ggccaaccgg aacctggagg aggccatggc ctctctgagg aaaatatccg agcggtgttc
     4921 ggtgacgctg ctggccacgc ccagcgcggt gaaagatttc ggcgccgaca aaatacgcgc
     4981 cgcggccggg gtgaacgacc tcctcgtggc cgacaaggcg ccttccgcgg tggacctgac
     5041 gcagtcccac gacgtgctgg tgttcgccac tctgtccatg aacgggctgt cgaaactgac
     5101 caccttcgcg gtggattcgt cggttttcat cacggcctat tatttcttcc aggccggcaa
     5161 gccgatcttc gcggcccgcg acggcctctg gtgcacgcgg ttccccgagc ccgccccggg
     5221 cagcccggcc ggccgcaagg ccgaggagat gttgaacggc ctgaaatcca tgggcgtccg
     5281 ggtctgttcc gctaaaacgc tgggctcgga ggtgttgtgg tcggtgggcg agccggggtt
     5341 cgcctcgtcc ctgggccgca ccaccgtcgg cacggcgggg aaagtctttc agggggccgc
     5401 ctcccccgcc cgtcccgcgg aggagcgggc gccggttccg gcgccgaagc cgctggagtc
     5461 ccacggcggc gagcataccg gcacggcgtc ctgcccgcat tgccagtcca tcggcaaact
     5521 gaacgcgccg gcgctgcagg cgttgggggc cgaacgcctc tccgtctgcc ggccctcgga
     5581 gaagggccac gaagtggcct tcctcatcga ccacaccctg ctgaaaccca acgccacgca
     5641 ggaggaggtc ggaaagcttt gcgaagaggc gaagaaatac tgttttgcgt cggtgtgcat
     5701 caacccggcg tacgtgcccc tggcggcgca gcttctgcgc ggcagcggcg tgcgcgtctg
     5761 cacggtgatc ggtttcccgt tggggtccac cacccccacg gtgaaggcca tcgaggcgcg
     5821 cgacgccatc gccaacgggg ccgatgagat cgacatggtc atcaacgtcg gcgcggtgaa
     5881 gtccggcaac tacgcgctgg tgctcgacga catccgggcc gttcgggacg ccacccgcgg
     5941 caaggtgctg aaggtgatca tcgagaccgc ctatctcacc cgggaggaga aggtcaaggc
     6001 ctgcgaactc tccaaggagg cgggcgcgga tttcgtcaag acctcgacgg gtttcggtcc
     6061 cggcggcgcc acggtggagg acatcgccct gatgcgctcc gtcgtggggc ccaccatggg
     6121 cgtgaaggcc tccggcggca tccgggacca ggagacggct ctgaagatgg tggaggccgg
     6181 cgccacgcgc atcggcgcct ccgcctcggt gtccatcacc atgggcaaga aggcgggcga
     6241 aggaaaatat tgagtgtgcg gcatatccgt gacaagggag gaatcagcga atggctgaac
     6301 agacaatgac ggaagcattg ggaatgatcg aaacgcgcgg cttggtcggc atgatcgagg
     6361 cgtcggacgc catggccaag gcggcgaagg tgaaactggt ggggttcgag aagatcgggt
     6421 cgggcctggt gaccacgctc tgtcgcggcg aagtgggcgc ggtgcgggcg gccgtggagg
     6481 ccggcgccgc cgccgctcag aaagtgggcg aactggtggc ggtgcacgtc atcccccggc
     6541 cgcacaacga cttggaaaaa tatgtccgca aggtcgaggg gagtttctag gtaggagcgg
     6601 ccggcgcggt ttcggcgcgc atgttgacgg aagacgacct gatcgacatc atcgccgacg
     6661 cgttggcgcg gcgactcggc ccggccgagt ggcgggacgg aaggtccgtt tcgccgtcgc
     6721 gccagggcaa gtgggggttt cacgtccaga aaccgttcaa ccggccctgg acggccctgc
     6781 cgatgaaacg gccgttcatc accgaataca tggcgcgcca aatggccggg cccgggcgtg
     6841 agatacgcat cccccggtcg gccatccttt ccccgttggc ggaggactgg ctgatggcgc
     6901 acggcgtcgc ggttgtgcgc gcggaacaat aaacgagagg ttcctatggc gatcgatgtg
     6961 actcaactct ttaagacgat ggtccgcacc gggatttccg acatccattt caaggcggga
     7021 acgccgccga tggtccgggt ccacggccgg atgatgtcgt cgggtttcac gaagatggcg
     7081 gcccagcata tcgaggagct ggcggatgtc ctgatgaaca ccgaacaaaa ggcgatgttc
     7141 gcccgcgaca aggagctgga catctccttc gaagtcccga acgtttcgcg cttccgcgtg
     7201 aacgtctacc gccagaaggg gaccatggcc ctctccctcc gggtcgtgcc gttgaccctc
     7261 cgcaccctgg ccgagttgaa cctgccggag gagaccctca aaaaactttg cgccaacacg
     7321 cgcggcctca tcctcgtcgc cggcatcacc ggcgccggga agaccaccac cctgaacgcc
     7381 ctcatcgatc atatcaacaa gacctacgcc tacaacatcg tcacggtgga ggatcccatc
     7441 gaatactacc acaccgacgc caaatcgtcg gtggcacagc gggaggtggg aggggacacg
     7501 gagtcgttcc tccgggccat gaagcacgtc ctccgtcagg atccggacgt ggtggtgctg
     7561 ggggagatgc gcgatgcgga ttccatccgc gcgggggtga tggcggcgga gacgggccat
     7621 ctcgttttgg gcaccatcca taccatgaac gccgcccaga cgctggaccg tcttctggag
     7681 gcctacgacg tcagcgaaca gatgggcgcc cgtcagcgca cggccaatat tctgcggggc
     7741 atcatttgcc agcgtcttct ggagaccgcc gacggcaacg ggcgggtccc ggccacggag
     7801 atcatggtgg tgacgtccct catacagaaa ctgatgcttg aggggaagac ggcggaactc
     7861 cgcaaggcca tcgaacaagg gaactattac ggcatgcact cgttcgacca ggacctgctc
     7921 cgcctggtca acgagaagaa gatcagcaag gaggaggccc tggacgcctc caacaacccg
     7981 gacgatctga tgatcaagct caacacgcag ggactgtccg gcggggccgg cccggcgtga
     8041 agcgcgtttc ggaaagaaga ggataggatc cgtgtccatt gagcgttggg aactcgttcc
     8101 atcgtcccgc gcggaccgct atctgttgag ggtgcgcggc gaccgcgccg aagtggacgc
     8161 catcgtccgc gcgtattccc gcgtctgcga ggagccccgc gagataagcg acccggtttt
     8221 tcagtgggcc atctacgtcg tcgacgccac cttgaacgaa cgcctggcga tccaggatca
     8281 gctgcggaac aggatgaccc gctccaccga accgtcgctg gtggctcccg tggacctgtc
     8341 gggcgtgttg gccgagcttt cgctggtttt ggaggaattg acgggcctga ccgacgagga
     8401 acaggcccac gtggcaaaga agatgcagga gatacagcag cgcgaggcgg aacaggccgc
     8461 cgcgcccctc cctccgccgc ccgtccctcc ccaagagccg gaaaggaaga tcgtgctcga
     8521 cgtggcgccc cgaacgccgc ccgtccgtga accggcgccg ccgaaggcgg ctcccgttcc
     8581 tcctccgccg tctccggaac ctccggcggt tcccaagcca tccgccgcgc cgtctttgcc
     8641 gccgtcctct cctccgccgg cttcgccgcc ccccgtggag gcggcttcgg tgagcgctcc
     8701 cctcccgccg gccccgcctt ctccggacgt gccccccgac cagctcttga gggcggtttt
     8761 cttttacgcc gaaggggcgg agaaccaaaa gaataagttc gtcgaaaaat tgacggacgt
     8821 ggcgcagaag aaggcgaaga aaccgctttt catccacccg gtcgcttccc gggcgacggc
     8881 cctcgtcgcc gccaaggcgg gggagtggat ccagacgtcc cggtcgagcg gagcggactg
     8941 ttttttcgtg ttgttgaccc cggacgtctt gccggatttt ttggagcggg ccgccaacga
     9001 ggcccgtcag tcggggctcc attgtttcct cgtccctcag gcggagctcg aatcgcggct
     9061 cctctacgtg gatttgatgg tcgaattgat gctgatcaaa cggaagagat aaggggcgcc
     9121 cggggacgaa cgaatgattg aacggtggga gattgtcgct gatccgcacg aacggttccc
     9181 gaatcggcac cgggtcagtg tggcggggga ggaatcggac gttcagtcgc ttttggcggc
     9241 gatggcccgc aaggcgggaa aaccgtgggc cctccggcgc ggggctttcc ggttcgcgtt
     9301 ttacctctat gatccgacgg aggaagaccg gcgccgggtc gaaaaatttt tggccggcga
     9361 ccgtccgacg gtttcgggaa aaacggcggt gtcgccggaa gggaggtccg ccctccctcc
     9421 agagtcctcg cccgtaaagc cgttttcatg ggaggaggcg ggggcggagg tggaggtcct
     9481 ctcggacctg ccgcccgccg ccggaaagcc ctcccgggaa gagacgaagc cgtccgcggg
     9541 gtcctccgat tgggagcggc cgtcgttggt ggaaagcgcc accgatgcgt tgatgtcttg
     9601 ggcggaaacc ggtccgaccg agaggaaacc ttcttccgtg ccgccggcct cccccgccga
     9661 gcctccgcct cccaccatcc aggtggaacg gacggaaaag aaagaagagg aggaggacgg
     9721 ggaggattct ccgccgtcgg gtctgatcgt cgccacggaa gagaccggca tgccgatgga
     9781 ggagatggtc gtctcttcgg cgcccgaacc cgaggtgccc aaggggtatt tgatgttccg
     9841 gctcggctat ttcatcccgc acgacatcgg aggggtggcg gatcgcatcc acagcctttt
     9901 ctcccagact ttggagacgc ggcacatgcc gttcgttttc cataaggtgt tcacctttcc
     9961 ctatcgatgg ccggaccgtc cgctggtgga tcaggtgatc gcgggctgcc agaaacacaa
    10021 gatagacggg ttggtctgcg tgggggacga aaagcgactg gaactcctgt tcgaacagtg
    10081 ccaagagaaa ggcatcaagc atcacttttt gcgccgggag gatacgcaga agaaatattg
    10141 gcggctgggg ctcatcaccc gcatcgttgt gacggactat tgacgaattt tttggcggac
    10201 atcgccgttt tttcaaaata caaacgatcc atccgaaagg gaaacggtta gcgtcgtgac
    10261 ggaaccccgc ggtttgccgg agactcgcct caacccacgc tacaccttcg aagaattcgt
    10321 ggtggggccg cacagccggt ttcctcacgc ggcggcccag gccgtttccg agaatctggg
    10381 caaggcctat aaccccctct tcatctacgg acccgtcggt gtcgggaaga cccatctcat
    10441 gcaggcggtg ggccaccggg tcctcggccg cgacggccag acccacgtcc tgtatatcac
    10501 cgcagaggaa ttcatgacgg atgtcatcga tctccttcgc agcgggaacc tcaagtcttt
    10561 ccgggataat tttcggtccc tcgaccttct catggtggac gacgtgcagt tcctttccgc
    10621 ctcggagtcc acgcaggagg agttcttcca caccttcaac gcgctccatc aggcgggtaa
    10681 gcagatcatt atgacgtcgg accgcccgcc caagatgctc accactctcg aagaccgcct
    10741 ccggagccgg ttcgagtggg gcctgatcgc ggacatcaag tcccccagcc tggagacccg
    10801 cgtcgccatc ctgcgcaaga aagagtccca gttcggcaat atgcaactgg gggaggacat
    10861 ccgcctctat atcgcgggga agctcaagtc caacgtgcgg gagctggaag ggttcctgcg
    10921 ccgcgtgcag gcctatgccc agctcaacaa tcagacgatc aatctccccg tggtgaagga
    10981 gatcatgcgc gagctcctgc ctcccagcag ttgggacgag gaggaccagg cggccttggc
    11041 cgagacgcaa cacccgggac acacgccgcc ggccggaggg ggaaaggccg gggtcgcccc
    11101 cgttctcctt ccgccggtct ccgctccccc cgccgccgcg ggggaatcgg cccatcgcgt
    11161 gaccccggcg atctctcccg acgccatcaa gatcgattcg gagatcagca tcgtgcttcc
    11221 cccggccgtg gaaccgcgcg tggagacggg ccccaaatcc gtggtggcgg cgtttttcta
    11281 ccccgcgggg aaagagaggg agctggccca gctaaaacaa aaattcgagg atgtgatcca
    11341 taagcataaa ttgaagttcc aactggacgc cgcgaccgag gcgccctatc cgttggagga
    11401 gactctctct tacgatacgt tcgtccaggc ctgcgcgcag aaatcttgcc acatcgcaat
    11461 cgtgctgggc ccgccgcccg gcagccgcct taaggatttc gatttccacc aaaaactgca
    11521 ggagcttttc gattcccacc gcgtttccct ccagttgatc ccgtgggaag agcttgggaa
    11581 agattaccgc ttcctgaaca tcgccctcga catcacgctg atccgctaca aagagaaagc
    11641 cccgtgatac ggccccgttt ttcggtcggg ttccagctgg cgctgttggc gtgcgtctcc
    11701 gtcgcggccg ccgtcggctt gtgggccggg ttgagcactg tcgccgtcaa gcgatacgtg
    11761 cgcgaggagg tctcccggcg ggcgtttcag tatgtcggcg aggtgtcccg tttggccggg
    11821 cggacggccc agggccgctc cgaaaaatgg gttgaaatga cgcccttgct ggaccggctc
    11881 gtcggggagc cgggggtccg ctatctcatc atctacggtc cgcagaaaaa aggcatcctg
    11941 gcgcagccct ccgtcccctc cccgctgaac gggacggagg aatcgttcac gatgccggtg
    12001 atggacgccg ccggccgcgt gatcgcttcg gcgcggctgt ggtattcgcc ggaggcctat
    12061 ttggggacgc tctgggaaaa aggacgcggc ccgctttttt tgctggcgct tttgaccggc
    12121 gtggggttgg gcggcgtgat ctatgcggga tgctggtggc tggtttttcg gccgatccgg
    12181 aaactcctcg acaggagcga aaacgccttc ctccccggaa aggccgccgc ccccctcgac
    12241 aagaacgagt tcgacgcctt ccttcgccgt ttccatgagt tgcggaagaa actggcggag
    12301 tctgaggaaa atttcagcgt gcttctctcc gcctccaaga ccatgacctc cgacatggac
    12361 attcaggaga tccacaaccg gattctggac ctggtctggc gccgtctgga ccgggacccc
    12421 tgtctgttgt ggtggcagga cgaggatgaa tttttgcgga tccgcaattt ccggaatttt
    12481 ccgccggaac ttatcaagcg gttgcgcctg aagcccggcg aatcgggggt ggggcaatgc
    12541 ctggtggccc ggacgcccgg cgtggagacc tccttggcca gcacgtccga aaaatggttg
    12601 gaggagctcc acgtctcctt ggatatcaac agcgcggccc atttccctct cttggtggac
    12661 gggaaaagcc tgggcgtgtt gtccgctttt tcgagacatg gggatttttt cagcgaggag
    12721 cggatgcgga tgatcgcctc cctcatggag tatctttctc tggccaccaa gagcgcgcgt
    12781 cttttcgaag agatgcagac gttcaaccgc cggatgaaat ccgagatcgc ctccaccacg
    12841 cgggaactgg ctcagaccaa cgtccgtttg atccggcgcg tgcgggagct ccgcgccctc
    12901 tacgacctgg ccgcccagcc ggagtcgccc tccacggaac atatgttttc caggacgctg
    12961 gaacatctcg tggatctgct ggaggtccgc cgttccgccg ttttcctctg ggacgagcac
    13021 gcccagcgct atcgttccgc cgtcgcgttc ctgtcggaaa acgccgtgtc cgtcgaatcg
    13081 ctgacgccgt ggatttcccg tcttcaggcg ggggaccccg tgttcttgga aagggccgag
    13141 gacgtccgga cgctttttcc ggactccgcc gccgtctcgc tcctcatgct ttcgcccatc
    13201 cgcgcggaga agtctctgct cggctttatc gccgccgccg acaagcgggg ggggcgcttc
    13261 gacgaggagg acgtccgcct tctggccctc ttcgcccggc atatcgcgga tatcctgcag
    13321 ggggtccagt tccggcggga acgcgagcag cggctgcgcg acctcaccct gttgcaggaa
    13381 gtgggcgcgg tgttgtcgag cgagccggac ctggaacaga ccctgcgcaa aacctccaag
    13441 gtggtggccg agtccctcgg cgcggacatc tgcgcttttc tcctctacga cgtccaaagc
    13501 gacgagttgg tgacccagcc gggagcctac ggtctttcgg aggacgcgga cgcctccatc
    13561 gtccgcatcc gcctcgacga tccgaagtcc gccaacgtgc gggtcttccg cacggggaag
    13621 gcgctcttga gccgcgacgt ggaggccgat ccggaggtcc gccctcatta cgccacgctg
    13681 tggaacatcc ggtcgctcat gctcatcccg ttgatcgccg agcagaagac catcggcgtg
    13741 ttgcgcttgg ggcacaaatc cccgaacgtt ttcaccgagg accatctccg gctggcccgc
    13801 ctcgtcgcgg aacaggccgc cgtgatcgtg cagaacgcca aactctaccg ccagatacgg
    13861 gagaacgtga ccgaactgcg gcggctcaac aacatcaagt ccgaattcct ctccatggtg
    13921 agtcatgaac tgaagacgcc gttgaccacc ctgaaggggt acgtcggcgt ccttctcaac
    13981 gagaaaaatg gtcccctgac ggcgcagcaa cagcgcttcc tgaacctggc caaattggcg
    14041 ctcaaccggt tctccatgtt gatctacgac ctggtggata gttccgccct ggaggcgggg
    14101 aaactcaaga tcgaaccggc ccccacggtc ctcggagaac tccttcagca ggcctatcag
    14161 gaccatctgc cggcggccca gttgaggaag atcaaccttg tgcttgagcc tctccccgac
    14221 gctctccccg ttgaggcgga cgcctcgcgc atccgccagg tggtggacaa cctggtcacc
    14281 aacgccttca agttcacgtc ggaggggggg gctgtgacgc tctccctgga ggaggtgccg
    14341 gaagggatgc gggtgagcgt gcgcgacagc ggcatcggc
//
LOCUS       DAOTFD010000047        13955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3025_length_13955_cov_5.167482, whole genome shotgun sequence.
ACCESSION   DAOTFD010000047 DAOTFD010000000
VERSION     DAOTFD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3025_length_13955_cov_5.167482"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5079_04555"
     CDS             complement(<1..605)
                     /locus_tag="P5079_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRY29292.1"
                     /translation="MRESTRFGALCAVLLAVSAAAFGEEVELGEAPSLDPYEISINPD
                     ENTAGTPVSVDVAEFGASTVTSVYFSPEDDCDNVIIGWLSQARRSIDAAVFLISHPGI
                     ADALAQAHLKGVKVRVIMDGEQADFKVASDEFLERAGVPLRRSFSKGSMHNKFAVIDG
                     RTVLTGSYNWTVGGASVNDENLIVLHQAAAVFQRKFDQLWNT"
     gene            complement(613..1122)
                     /locus_tag="P5079_04560"
     CDS             complement(613..1122)
                     /locus_tag="P5079_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29293.1"
                     /translation="MDDKTMNPPAPSGPERRKLPDAPTRKVLFLWIALMPAVLWVLQK
                     FFHERFYQYDGQRWGLSPLGSACVFGAAALLAAGLCSAFYKVKYGKFHFIAVARVVRG
                     ILSLFLIAVVFVWPIAMMIVAINPVHAAYLAVLVALGIAGLTILIRLAMPPEAGFWRD
                     WIHDTLLRW"
     gene            complement(1428..2615)
                     /locus_tag="P5079_04565"
     CDS             complement(1428..2615)
                     /locus_tag="P5079_04565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29294.1"
                     /translation="MGDLSGPDPIRVAVFSYTLDDPTCHRYLDMLAAAERPSHIKLTV
                     WTTFRPTAKTLRSWRPRLSWHSFFSSRRRPSPRRYARVFADHLRRENVSIVHWAFLRE
                     LGFSAWRALTRAARPQARTVWTHHIVSEMTAAYRKSAAFRGLDRIVVLSRAMKKHLLA
                     DHPAGPPSRFRVIPNGTDASYWRRGGFSGKRDRRLFLAVARINADKGLHQLLWAFCGI
                     QKEFPDARLFVAGKVDLQPDYYRRLKRLAEKLGVRSHVTFLGEVTPETLRRLYHRSLC
                     CVQPSLNEGVSFAALEACAAGCPLVLTRTGGIEEILRHGRDCLFVPKGDPDLLQKAMR
                     RMLVEPGLRERLARNARRRALAQDERAVSRAYIRLYEELAAEGRSARGFERRLEPKTA
                     EFL"
     gene            complement(2615..4600)
                     /locus_tag="P5079_04570"
     CDS             complement(2615..4600)
                     /locus_tag="P5079_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY29295.1"
                     /translation="MRPPRSRAVRVALVVMPFQPVETPNLGASILKSRLRRAGVECRV
                     LYFNVEFARIVGLERYARLLGLKIAPEILFSHALFGKAGTREIRYPRPLGAVLTKAGM
                     FGEEIRAGLRGDMAAVERLAERQAPRFREACLSAASWGDYDVVGFSSIFNSHVASLGL
                     ARDIKERHPDTKIVFGGSNVEGIMGRETLRAFPWVDFVVGGEAEDTFPALLSALKAGR
                     SPDAIPGVSYRDARGLHGNHSLPPPVDLGESVPPDFSDYFGALERGGLRKSLRPVVVF
                     ESARGCWWGERGPCAFCGLNGLSMTFRRKPPRKILREIFDAMRRYRVSDFHAADRVWD
                     PAHATTFLPLLSRELKKRRLDVGMLYEVRPTITKKHLDLLARAGVTDVQPGLETLDTA
                     ILKRMNKGTTALRNVQTLKHCREADIFPRWNFLYGLPGEKASAYRRMARLLPKIQHLP
                     PPTMVTPVRIERFSPLHRHPGRFGLKNLRPAALYGWAYPQSRVRLKDLAYYFEGQFRK
                     DCVPPWAAIASFRRRLERWNALSRHSFLFHRHAWDSLIVYDHRPRSLSQGVRPFRIRR
                     YEGTSSAEIYEACAAGRTTREVERTLKRRRPKGRFPQVEIRRALRRFVRDGIMLEEGG
                     RFLALSLPFAACPPAVKRKVMLLEKMRGIFHEGRRAE"
     gene            complement(4597..5733)
                     /locus_tag="P5079_04575"
     CDS             complement(4597..5733)
                     /locus_tag="P5079_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29296.1"
                     /translation="MKNVFYDFVERVVREKTGHFINWPLAGVLARKKLWRKLFNYLIV
                     QAQFHFQTSWVFGRPYYLCFDPVTACNLKCPLCKIGTMEFPKPRERMDMAVFRKILDT
                     LGPELLQVDIYVHGEPLLNPDVYEMISYCKKYKTTVKMSSNLQEVDADRLVGSGLDQL
                     FVAIDGATQETYEKYRMGGSLEKAFTNARNILAARRRAGKKTPLVTWKYIVFRHNEAE
                     IDLAKKAAAEMGFDEIDFNGAIVKDAADPTQMHTWVPQNPEHSLYAPPATAPSPAAPA
                     LQKSVDLSVRKPQCNWPWISATLDPRGNVQTCCVSVRPEEDMGNIMESRWGAIWNNAR
                     YRRARRFLARHRPSAAGWNIPCERCRAAGNINLSLTEHLLRRGL"
     gene            complement(5748..6977)
                     /locus_tag="P5079_04580"
     CDS             complement(5748..6977)
                     /locus_tag="P5079_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29297.1"
                     /translation="MRTRDRLVAECLSMGARAHGGNPNKIHAGPLSPGCRLCAEGKWS
                     CLFMGRFCRRGCFFCLQSQREKKAALREKPTGFRPRSAEEYREFVRRAGYRGVAFSGG
                     EPLWFFDELIEYVTALRKGAGPRLAIWVYTSGDFMTERRLRELKSAGVDELRFNIAAR
                     NYSLTALSRAAKHFENVTVEIPAIPEDTTRLLALLPRLRSAGVKYLNLHQLTASAHNS
                     DALARRGYRIHKNGEGYPAVGPVVESELAALRLLREGLASGPPLPIHYCSARYKELFQ
                     ARAHRQRLAEYFTGETAALRRASKVTSAGYLRRRVPDDGRRTVPSKVCYLDFRSPADS
                     GNEHGVFSRGRIVKEVPLKSAGERRLFEKFFLSAVADESVVGAILAENKKDSGVARFL
                     RRFENLEYLPVSLDRYC"
     gene            complement(7101..8168)
                     /locus_tag="P5079_04585"
     CDS             complement(7101..8168)
                     /locus_tag="P5079_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29298.1"
                     /translation="MSPSCRLCLRGKSTSFKLTVQCNRGCFYCPNLKGDKNTPRPENF
                     IAYQAPSLKEVIRSVEDARSRGCSITGGEPFLEFPRTLVYIRALRRRFGPRFRIHLYT
                     NGLLATDRRLRRLKRAGLDEIRFDLAAIGYKTAPVYRALKYFEHVGVEIPAVPGDEEK
                     VKKIMLELDAAGVRYLNLNELYFIRANVSRLKEHGFSAVCEGRRPVHDGFEGRMYPVK
                     ESEPAAARLLAFAAKNLRRLSVHYCTCAAKRNNHDQQLKRLARTCRKSHERIDPTGLL
                     EKLVVRTDDPRRTERELLAAGFPRKRIHYSRSKKRLEASVDCAKRLSSRGYLYALVKV
                     LPADAFADVSEHPLESGAGRL"
     gene            8591..11287
                     /locus_tag="P5079_04590"
     CDS             8591..11287
                     /locus_tag="P5079_04590"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRY29299.1"
                     /translation="MKKALLLTAVVIGILVFGILGLTLFLQWRFPPPKLAEIAGEQAR
                     RHLNRELRFDAVSVGLFRGLRLDGFTLSEPPNLQAGTFISGKSLSVKPRLWPLLRGDL
                     VLKSLTLEKPDIRLIRFADGSYNYSDLLSSGTAPAVVPAPGSVPRSEAAPSLFIQQLD
                     VRKADIRFVDKSSAAVNLSMTDMDLSVSGFSLTTPGRIRLAGRLNGSLSGKPFEAQVL
                     LRGTLLPSPNGSLELDEFRLKTGKTTVSAGGRITDFHSPSIDLRLHPEPLRAQDLVPF
                     FSMPIGLSDMDLRGELHLNGNLEKMSIRGDLSLRGAGLTAAVAPDLSLTDPLKKIGFA
                     GVLAVSNLSAQKSPLFPDLSLTGPLQGKVEARGTLKQATMSFHFTGDRATFRYASLVK
                     KRENAPLRLDGKITIKDPVGGSPLDVRGEFGGLELPTSAPPLPADLRLSGPLSAKINV
                     GGTLDRPSFRFQLLADEAAVSYAGYFQKPSGKPLALLADVRLSDRKNLLLKNFQLSME
                     SLSLTGHGNISDLPGTADMGLSFKIPPVSLESLSPAVPLIKEYGLSGRTSLDLQLAGT
                     LAAPRISGSLTLKNFGAVPKAGLVFSEFEGDVAFSMDSMETSLLKGRFNGEPLLVRSQ
                     LKNFSKPELWCEGELATLDAGKLQKVFSETSSSAVPVRPAGAPAATEPALSLAKAEGH
                     FKIETLSHPNYIGRRFRLAWNLTEVDSEMLHWNGTAELSADDGKIYNLPLAVKINKIL
                     KKDAADLTYSRLSSHFQMKDGLLETRDFMIDSSQMDIAVRGTLKLSTLLADLRATFKL
                     PMESALGTVGDWVIGEDGALTLEAVIKGPLTDPAVAVDLDSAARQAAQNLLKKGVERL
                     KDSLQSPQDKDQKPSLPVQGEKQILKTIEQGQKALDKLFKKK"
     gene            11284..12450
                     /locus_tag="P5079_04595"
     CDS             11284..12450
                     /locus_tag="P5079_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29300.1"
                     /translation="MTGSIPPTSIKRRRTLSLFVLLAVFVPVLPLRGAEKKALSLEAD
                     PLIFSPNGDGRKDAVYFLFGTQGLENIHKWRIVLYDALGRRVWRDGGRGTPPGLLEWA
                     GVTLAHKTAREGVYVAQARVWASRNKAASPAVDLVVDLTPPLATVELSTPIFSPGPEQ
                     AVGFLLRAEDSIGLSHWELRIFSDDTPTLSIFSSTGGFENGAASPSWDGRTQQTQSFA
                     DNGSYRTEFIVEDAAGNQQTLSSPLQISVDSTTALASAASAMKITPLPDGGFKVIAPS
                     DSLFDPANSSSLKAAGPDSALNQLFSLAKSHPTHRLLLNGYAGSEKTSTRRVDLSSAQ
                     VWAIYSALVKGGVSATRLEVHGLGGKFRDVNRIEILFTAPPAPPPDPLPDAPAP"
     gene            12465..13934
                     /locus_tag="P5079_04600"
     CDS             12465..13934
                     /locus_tag="P5079_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HRY29301.1"
                     /translation="MTADAGSSKPLVLLTGATGYIGGCLLSLLEHDHFPVRCLVRREL
                     VRRGSPDTTSTVLGDVLDSQSLRRAMDGVHTAYYFIHSLKARENFEAEERRGAQNFAF
                     AAKAAGVRRIIYLGGLGDPHDTLSPHLKSRRAVGDVLRSSGVPVIEFRASIVIGAGSL
                     SFELIRCLVQRLPIMLAPRWVTNLTQPISIQDLLAYFRAALELPEDGHRTFEIGGRDV
                     VSYGDILREYARQRGLGRLILPVPLLPRPLSCLWLKLVVPEYAAVGEKLFESLRNPTV
                     VSDHAATRVFGLRPTGFREALATALSREDDSFRRSRWGESPAIKEAPGRKGVRIGSRI
                     LDCRTVRLDVPAASAFAPIQRIGGTRGWYHADWLWQFRGFLDELVGGVGMRRGRRHPE
                     DLAVGDVLDCYRVEYVEPGRELLLRAELRLPGRAWLQFEVSGEGSSSEICQSAIFDPK
                     GLFGFLSWYALYPFHKYIFSGMLEKIAAAARREHASRTH"
BASE COUNT         2635 a         4775 c         4019 g         2526 t
ORIGIN      
        1 gtgttccaaa gctggtcgaa cttcctttgg aacacggcgg cggcctgatg caggacgatg
       61 agattctcgt cgttgacgga ggctccgccc accgtccagt tgtagctccc ggtcaacacc
      121 gtccggccgt cgatgacggc gaatttgttg tgcatgctcc ccttcgaaaa actccgccgc
      181 agcggcaccc cggcccgctc cagaaattcg tccgacgcga ccttgaaatc cgcctgctcc
      241 ccgtccatga tgacccggac cttcacgccc ttcaggtggg cctgcgccag ggcgtcggcg
      301 atgccgggat ggctgatgag gaagaccgcc gcgtcgatgg agcggcgcgc ctgggaaagc
      361 cagccgatga tgacgttgtc gcaatcgtct tccggtgaga aatagacgct ggtgacggtg
      421 gaggccccga actccgccac gtccacgctc acgggcgtcc cggcggtgtt ctcgtcggga
      481 ttgatggaaa tctcgtaggg atccagggac ggggcctcgc ccagctccac ttcctccccg
      541 aacgcggcgg cggacacggc caagagaacc gcgcacagcg ctccgaaacg tgtgctctcc
      601 ctcatgccga tatcaccatc gaagcagggt gtcgtgaatc cagtcccgcc agaaccccgc
      661 ctccggcggc atcgccagcc ggatgaggat cgtcagcccc gcgatcccca gcgccaccag
      721 caccgccaga taggcggcgt ggacgggatt gatggccacg atcatcatgg cgatcggcca
      781 gacgaaaaca acggcgatga ggaaaagcga gaggatgccg cgcaccacac gggccacggc
      841 gatgaaatgg aacttcccat acttcacctt gtaaaaagcg gaacacagcc ccgccgccag
      901 caacgccgcc gcgccgaaca cgcaagcgct ccccagcggc gaaagtcccc aacgctgtcc
      961 gtcgtattga tagaaccgtt cgtggaagaa cttctgcagg acccacaaca ccgccggcat
     1021 gagcgcaatc cagagaaaaa gcactttgcg cgtgggcgcg tcgggcagtt tgcggcgttc
     1081 cggtccggac ggcgccgggg gattcattgt tttatcgtcc atggtctctc cgtttcgtca
     1141 catgaggagt atcccgtgag ggccagcagc ttctcgactc cctccggccc ccaccaccga
     1201 acacgtgccc cgtccgacaa accgggacgt tgcccagcgg caacgcgggg cctgagttac
     1261 aggtcccgcc gtcgcggcgg aggtgttgcg gggccgtccc tcttccgtct gaggttggga
     1321 cggtcgctcg aagaacaacc gcgttgttcg agcagggcgc agcgaagtcg agcccggtgg
     1381 ggggctgaaa tcaatttact tgttttcccg agatgccccc cgtcacatca taggaattcc
     1441 gcggttttcg gttcaagtct cctctcgaaa ccccgtgcgg accgcccctc ggcggccaac
     1501 tcctcgtaaa gccggatgta ggcgcgcgac accgcccgct catcctgcgc aagcgcccgg
     1561 cggcgggcgt tccgggccaa gcgctccctc agtcccggtt ccaccaacat ccgccgcatc
     1621 gccttctgca agaggtcggg gtcccccttc ggcacaaaaa ggcagtcccg cccgtgccgt
     1681 aaaatctcct cgatccctcc ggtccgcgtc aaaaccagcg ggcatcccgc cgcgcacgcc
     1741 tcgagggccg cgaaactgac cccttcgttc agcgaaggct ggacacagca caggctccgg
     1801 tgatagagac ggcgcaacgt ctccggcgtg acctcgccca aaaaagtcac gtggctccgc
     1861 actcccagct tctccgccaa ccttttcagc cgacggtaat aatccggctg aagatcgacc
     1921 ttccccgcga cgaagaggcg cgcgtccgga aattcttttt ggatcccgca aaacgcccac
     1981 agcagctgat ggagaccctt gtccgcgttg atccgagcga ccgccagaaa aagccggcgg
     2041 tcccttttcc cggaaaaccc gccccggcgc caataggacg cgtccgtgcc gttggggatg
     2101 acccggaagc gggacggcgg tccggcgggg tgatcggcga gcaaatgctt tttcatcgcc
     2161 cgagaaagga cgacgatcct gtccagtccc cggaaggccg ctgatttccg ataggccgcc
     2221 gtcatctcgg aaacgatgtg gtgggtccag accgtccttg cctggggccg ggccgcccgg
     2281 gtcaacgccc gccaggcgga aaaaccgagc tccctcaaaa atgcccaatg cacgatggaa
     2341 acgttctccc gccgaaggtg atcggcgaag acccgggcgt aacggcgcgg gctcggacgg
     2401 cggcgggagg aaaaaaaact gtgccaggaa aggcgcgggc gccaggagcg cagcgtcttc
     2461 gccgtcggtc ggaaggtggt ccacacggtc agttttatgt gggaaggccg ctcggccgcg
     2521 gccaacatgt ccaggtagcg atggcaggtc gggtcgtcca gcgtatagga gaaaaccgcc
     2581 acacggatcg ggtccgggcc cgaaaggtcg cccattattc ggcccgtctc ccctcatgga
     2641 aaatgccgcg catcttttcc aggagcatca ctttcctttt aacggccggg ggacaggcgg
     2701 cgaacggcag cgacagcgcg aggaagcggc cgccttcctc cagcatgatc ccgtcccgca
     2761 cgaagcggcg caacgcccgc cgaatctcta cttgaggaaa cctccccttc gggcgtcgac
     2821 gtttcaatgt ccgctccacc tcccgggtcg tccggccggc ggcgcacgcc tcgtatatct
     2881 cggcggacga cgtcccctca tagcggcgta tccggaaagg gcgcaccccc tgggaaaggg
     2941 agcgcggccg atggtcgtaa acgatcaacg aatcccaggc atgccggtgg aacaaaaagc
     3001 tgtggcggga gagggcgttc caccgctcca ggcgtctccg gaacgacgcg atcgccgccc
     3061 agggggggac gcagtccttc cggaattggc cttcaaaata atacgccagg tctttgagac
     3121 ggacccggct ttggggatag gcccacccgt aaagcgcggc ggggcggagg ttcttcaatc
     3181 cgaaacgccc cgggtgccgg tgcaaggggc tgaacctttc gatgcgcacc ggcgtcacca
     3241 tcgtgggcgg cgggagatgc tgtatcttcg gcaacagccg ggccatgcgc cgatacgcgg
     3301 acgctttttc gccgggcagg ccgtaaagga aattccagcg cggaaagatg tccgcctccc
     3361 ggcagtgttt caacgtctgc acgttccgaa gggccgtcgt ccccttgttc atccgcttca
     3421 ggatggcggt atcgagagtt tccaggccgg gctgcacgtc ggtgacgccc gcccgggcga
     3481 gaaggtccag atgttttttc gttatggtgg gccgcacctc gtagagcatg cccacatcga
     3541 gccttctctt cttcagttcg cgggagagga gcggcaaaaa cgtcgtcgcg tgggcgggat
     3601 cccagacgcg gtccgcggcg tgaaaatcgc tgacgcggta tctcctcatc gcgtcgaaaa
     3661 tctcccgcag gatcttccgg ggcggcttcc ggcggaacgt catcgacagc ccgttcagcc
     3721 cgcagaacgc gcagggaccg cgctcccccc accaacaacc gcgggcgctc tcgaaaacga
     3781 cgaccggccg cagggatttc cgcagccccc ctctttccaa ggcgccgaag taatccgaga
     3841 aatccggcgg gacgctctcc cccaggtcga ccggcggcgg caaggaatgg ttcccgtgga
     3901 gaccccgggc gtcccgataa gaaacgccgg ggatcgcgtc gggggaacgt cccgctttca
     3961 acgccgacaa cagggcggga aacgtatcct ccgcctcccc gccgaccaca aaatcgaccc
     4021 agggaaacgc ccgcagcgtt tcccgtccca tgatcccctc cacgttggat ccgccgaaaa
     4081 cgattttggt atcgggatgc cgttccttta tgtcccgcgc caaccccagt gacgcgacgt
     4141 ggctgttgaa aatgctcgag aaaccgacca cgtcgtagtc gccccaagac gcggccgaca
     4201 ggcaggcttc ccggaaccgc ggggcctgcc gttccgccaa cctctcgacg gccgccatgt
     4261 ccccgcgcag gcccgcccgt atctcctcgc cgaacatccc cgccttcgtc aggaccgccc
     4321 ccagcggccg gggatatcgg atttcccgcg tcccggcttt cccgaaaagg gcatgagaaa
     4381 aaaggatctc cggcgctatc ttcagaccga ggaggcgcgc gtatctctcc aggccgacga
     4441 tccgggcgaa ctccacattg aaataaagga cgcgacattc cacccccgcc cggcgcagac
     4501 ggcttttcag gatggaggcc ccgagattgg gcgtctccac gggctggaac ggcatgacga
     4561 cgagcgccac ccgcacggca cgagaacgcg gcggcctcac agccccctcc gcagcaagtg
     4621 ttcggtcagg gacagattga tgttcccggc cgcccgacac cgctcgcacg ggatgttcca
     4681 tccggccgcg gagggacgat gccgggccag aaaacggcgg gcgcgacggt atcgcgcgtt
     4741 gttccatatc gccccccagc gcgattccat gatgttgccc atgtcctctt cgggccggac
     4801 ggagacgcaa caggtttgca cgttgccgcg cggatccagg gtggccgaga tccacggcca
     4861 attgcattgc ggtttcctca cgctcaagtc gacgctcttc tgcaaggcgg gggcggcggg
     4921 agacggcgcg gtcgccggcg gggcatagag gctgtgttcc gggttttgcg ggacccacgt
     4981 gtgcatctgg gtcggatcgg cggcgtcctt cacaatggcc ccgttaaaat cgatctcatc
     5041 gaaccccatc tcggccgccg cctttttcgc caaatcgatc tcggcctcgt tgtgccggaa
     5101 gacgatgtat ttccacgtca cgagcggcgt cttttttccc gcccggcggc gggccgccag
     5161 gatgttccgc gcgttggtga aggccttctc cagcgaccca cccatccggt acttttcata
     5221 ggtctcctgg gtcgccccgt cgatcgccac gaagagctgg tccaggccgg agccgacgag
     5281 acggtcggcg tccacctcct gaaggttgct gctcatcttg accgtcgttt tgtatttctt
     5341 gcagtacgag atcatttcgt aaacgtcggg gttcagcaac ggctccccgt gcacgtagat
     5401 gtcgacctgc agcagttccg gccccagcgt gtccaatatc tttcgaaaga cggccatgtc
     5461 catgcgctcg cgcggctttg ggaactccat cgtcccgatc ttgcagagcg ggcacttcaa
     5521 attgcacgcg gtcacgggat cgaaacacag gtagtagggt cggccgaaga cccacgacgt
     5581 ctgaaagtgg aactgcgcct gcacgatcag gtagttgaac agtttccgcc acaatttttt
     5641 ccgcgccaaa accccggcga gcggccagtt gatgaaatgg cccgtctttt cccggacaac
     5701 ccgctcaacg aagtcgtaga agacgttctt cacgtcgccc cccggcgtca acaataccgg
     5761 tccaaggaaa ccggcagata ttccagattc tcaaatcgcc gcaggaaccg cgcaaccccg
     5821 gaatcctttt tgttttccgc aaggatcgcc ccgacgacgg actcgtccgc caccgccgac
     5881 agaaagaatt tctcaaagag acgccgttcc ccggcggact tcaacggaac ctctttgacg
     5941 atcctccccc ggctaaaaac gccgtgttcg tttcccgaat cggcggggga acggaaatcg
     6001 agatagcaca ccttcgacgg aaccgtccgc cggccgtcgt cggggacccg tcgccgcaga
     6061 tatcccgcgg acgtcacctt cgacgcgcgc cgaagtgccg ccgtttcgcc ggtgaaatat
     6121 tcggccaacc gctgacggtg cgcgcgcgcc tggaaaagtt ccttatagcg ggcggaacaa
     6181 tagtggatgg gcaggggcgg gccggacgcc aacccctcgc gcaggaggcg caacgccgcc
     6241 agttcggatt ccacgacggg gcccaccgcc gggtacccct ccccgttttt gtggatccga
     6301 tagccccgac gggccaaggc gtcgctgttg tgggcggacg ccgtcagctg gtgcaaattc
     6361 agatatttga cccccgccga acgcaaccgg ggcaacagcg ccaaaagccg ggtcgtgtcc
     6421 tccgggatgg cggggatttc aacggtcaca ttctcgaagt gtttggccgc ccgggagagc
     6481 gcggtcaggc tgtaattccg cgcggcgatg ttgaaccgca actcgtccac cccggcgctc
     6541 ttcaattccc tcaacctccg ctcggtcata aaatcgccgc tcgtatagac ccagatggcg
     6601 agacgcgggc cggcgccttt ccgcaaagcc gtcacatact cgatcaattc gtcgaagaac
     6661 cagaggggtt cgccccccga aaaagccact ccccgatatc cggcccggcg gacgaattca
     6721 cggtattcct cggcggagcg gggcctaaaa ccggtgggct tttcgcggag cgccgccttt
     6781 ttctcccgct gcgactgcag gcagaaaaaa cagcctcgac ggcaaaaccg ccccatgaac
     6841 aggcaggacc acttcccttc ggcgcacaac cggcatcccg gagacagcgg cccggcatgg
     6901 attttattcg gattcccgcc atgcgcccgg gcgcccatgg acaggcactc ggcgacaagc
     6961 cggtcccgcg ttcgcacgag accgcgcgcc tcgcgagact ttaccgaggg gcaaccgcaa
     7021 aaatcgaacc gtttttcgcg ttcaagggag ggcgggaaca tgggaagaat gattgtcgcg
     7081 ggaggcggtc ggcgaacggg ctagagccgc cccgcccccg attccagagg atgttcggaa
     7141 acatccgcga acgcgtcggc cggcaggacc ttgaccaagg cgtatagata acctcgggag
     7201 gaaagcctct tggcgcaatc cacgctcgcc tccaaacgct tcttggaccg ggaataatgt
     7261 atccgtttcc tcgggaatcc ggccgccaga agttctcttt ccgtccggcg ggggtcgtcc
     7321 gtgcgcacca ccagtttttc aagcaatccg gtcgggtcta tccgttcatg gcttttgcgg
     7381 caggttcggg cgagccgctt cagctgctga tcgtggttgt tccgttttgc ggcgcaggtg
     7441 cagtaatgca ccgaaagccg ccggaggttc tttgcggcaa aggccagcaa gcgggcggcg
     7501 gccggctcgc tttccttgac cggatacatt ctcccctcga acccgtcgtg cacggggcgc
     7561 ctcccctcgc agaccgcgga aaaaccatgc tccttcaatc gcgaaacgtt cgcccgtatg
     7621 aaatagagtt cgttcagatt gagatagcgg acccccgccg cgtccagctc cagcattatt
     7681 ttttttacct tttcctcatc gcccggcacc gcggggatct cgacgccgac gtgctcaaaa
     7741 tatttcaagg cccggtagac gggggcggtc ttgtacccga tggccgccag gtcaaaacgg
     7801 atctcgtcca gcccggcgcg cttcaaccgc ctcaatctcc ggtcggtggc caggagtccg
     7861 ttggtgtaga gatggatgcg gaaccgcggg ccgaaccggc ggcgcaaggc ccggatgtac
     7921 accagcgtcc gcgggaattc caggaacggt tctcccccgg tgatgctgca cccccgagaa
     7981 cgggcgtcct cgacggatct gatcacctct ttcaacgacg gggcctggta cgcaatgaag
     8041 ttttcggggc gcggagtgtt tttgtcccct ttcaggttcg gacaataaaa acagcccctg
     8101 ttgcattgga cggtcaactt gaaactcgtg gatttccctc tcaaacagag ccgacaagac
     8161 ggggacattt gccccacgta caacgttttt ccgccgttca gctcccgcac ccctccgcgc
     8221 agcagagacc tcaataattt ccgtttccgc aggggggcgc ggtgaacctc gcggaggcct
     8281 tcgtagcgcg ctcgatttaa ccgaacggcc tcctggagat acctcaactg tttggatttt
     8341 ggccctgaca cggattcccg tcctccagaa acctgtctct tgattcatca cggttgactc
     8401 gcggaacata tcgccaaaac gaaagggctt ggggatgttc gatggaactg aaaccgaggt
     8461 aaaattataa caaatctgtc gccgcggaac gggtaactct cgataaaaat ggtctataat
     8521 ttatctttaa aagcgcgccc gcggccggac gccatcccgt atcctttcgt cggagtctcc
     8581 tgtccccgat atgaaaaaag cgctgctcct caccgccgtc gttatcggga tcctggtctt
     8641 cgggatcctg gggctgaccc tgttcctcca atggcgtttc cctcctccga aattggcgga
     8701 gatcgccgga gaacaggccc gccgacattt gaaccgcgaa ctgcggttcg atgccgtctc
     8761 cgtgggactc ttccggggcc tccgcctcga tggattcacc ctttccgaac cgccgaacct
     8821 gcaggccgga acgttcatct cgggaaaatc cctgtcggtg aaaccccgtc tttggccgct
     8881 cctgcgcggc gacctcgtcc tcaaaagcct gactttggaa aaaccggata tccgcctcat
     8941 ccgtttcgcc gacggttcct ataattactc ggacctcctg tcttcgggca cggcgccggc
     9001 cgtcgtcccc gcgcccggga gcgttccccg atccgaagcg gccccgtccc tttttattca
     9061 acaactcgac gttcggaaag cggacatccg cttcgtggac aaatcctccg ccgccgtgaa
     9121 cctgtcgatg acggacatgg acctctcggt ttccggtttt tcgttgacca cccccggtcg
     9181 catccgcctc gccgggcgtc tcaacgggtc cctctccgga aaaccgttcg aagcgcaggt
     9241 cctcctgcgg ggaacgctgc tcccctcgcc gaacggctcc ctcgagctgg atgagttccg
     9301 tctaaaaacc gggaaaacga ccgtctccgc cggcggccgg ataaccgact tccattcccc
     9361 ctcgatcgat ctccggctcc atccggaacc gctgagggcg caagacctcg ttcccttttt
     9421 ctccatgccc atcggcctct cggacatgga cctccgcggc gaactgcatc tgaacggcaa
     9481 cctggaaaaa atgtcgattc ggggggacct ctccctccgc ggcgccggcc tgacggccgc
     9541 cgtggccccc gatctctctc tgaccgaccc gctgaaaaaa atcggctttg ccggcgtcct
     9601 ggccgtctcc aacctgagcg cgcaaaagtc ccccctcttt cccgacctct ccctcaccgg
     9661 ccccctgcaa gggaaagtgg aggcgcgcgg caccctaaaa caagcgacga tgtccttcca
     9721 tttcaccggc gaccgggcga cgttccgcta cgcttccctg gtgaaaaaac gggaaaacgc
     9781 ccctttgcgt ctggacggca aaatcacgat aaaagacccc gtgggcggtt ctcccctcga
     9841 tgtccgcggg gagttcggcg gactcgaact accgacaagc gccccccctc tccccgccga
     9901 cctgcgcctt tccggccccc tgagcgcaaa aataaacgtg ggcggcaccc tggaccggcc
     9961 ctcgttccgc ttccaacttc tcgccgacga ggcggccgtc tcctatgccg gctatttcca
    10021 aaaaccttcc ggaaaacccc tcgccctcct ggccgacgtt cggttgtccg accgcaagaa
    10081 tctcctgctg aaaaatttcc aactgtcgat ggagtccctg tccctcacgg gccacggaaa
    10141 catttcggac ctccccggaa cagccgacat ggggctctcc ttcaaaatcc ccccggtctc
    10201 ccttgagtcg ctctctccgg cggtcccgct cataaaagaa tacggccttt cgggccggac
    10261 ctcgctggat ctccaactcg ccggcacgct cgccgccccg cggatcagcg gctccctgac
    10321 gctcaaaaat ttcggggcgg tccccaaggc cgggctcgtc ttctcggagt tcgaaggcga
    10381 cgtggcgttt tccatggatt cgatggaaac ctctctcctg aaaggccgat tcaacggcga
    10441 gcccctcctc gtccgctccc agctgaagaa cttctccaaa ccggaactct ggtgcgaagg
    10501 cgagctcgca acgctggatg ccggaaagct gcagaaggtt ttttctgaaa catcctcatc
    10561 ggccgttccc gtccgaccgg ccggcgcgcc ggccgcgacg gaaccggccc tttcgttggc
    10621 caaagccgag ggccatttca agatagagac cctctcccac cccaattaca tcggccgccg
    10681 gttccgcctg gcgtggaacc tgaccgaggt cgattccgag atgctccact ggaacggcac
    10741 ggcggagctc tccgccgacg acggtaaaat ttataacctc ccgttggccg tcaaaattaa
    10801 taaaatccta aaaaaggacg ccgccgacct cacctacagc cgcctgtcgt ctcatttcca
    10861 aatgaaggac ggcctcctag aaacgcggga tttcatgatc gacagttcac agatggacat
    10921 cgccgtccgc gggacgctga aactctccac cctgctcgcg gacctgcgcg ccacgttcaa
    10981 actgccgatg gaatccgccc tcgggaccgt cggtgactgg gtcatcggag aagacggcgc
    11041 tctcacgctg gaggccgtca tcaagggtcc cctgacggac cccgccgtcg ccgtggacct
    11101 ggacagcgcc gctcgacaag cggcccagaa cctcttgaag aaaggcgtgg aacggttgaa
    11161 agactccctc caatccccgc aggacaagga tcagaaacct tcgcttcccg tacagggcga
    11221 aaaacaaata ttgaaaacca tcgaacaggg ccaaaaagcc ctggacaaac ttttcaagaa
    11281 aaaatgaccg gctcgatccc gcccacttcg atcaaacgcc gacggacgct ttccctgttc
    11341 gtcctcctgg ccgtcttcgt tcccgtcctc cctctccgag gggccgaaaa gaaagctctc
    11401 tccctggaag cggacccgct gattttttca ccgaacggcg acggccggaa ggatgccgtc
    11461 tactttctct tcggcacaca gggtctcgag aacatccaca agtggcgcat cgtgctttac
    11521 gacgccttgg gaagacgggt atggcgcgac ggcggacggg gaacacctcc cggacttctc
    11581 gaatgggccg gggtcacgct cgcccacaaa accgcccgag aaggcgtcta tgtcgctcaa
    11641 gcccgcgtat gggcgtcccg aaacaaggcc gcctcccccg ccgtcgacct cgtcgtggat
    11701 ttgacccctc ccctcgccac ggtggaactc tccacgccca tcttttctcc cggcccggag
    11761 caagcggtcg gttttcttct tcgggcggaa gactcgatcg gcctgtccca ctgggagctg
    11821 cgtatttttt ccgacgacac cccaaccctg tccatcttct cctccacggg cggcttcgaa
    11881 aacggggcgg cgtccccgag ctgggacggc cgcacccagc agacccaatc ctttgcagac
    11941 aacggctcct accggaccga attcatcgtg gaagacgccg ccgggaatca acaaacgctt
    12001 tcttcgccgc tccaaatttc cgtcgactcg accacggccc tggccagcgc cgcctcggcg
    12061 atgaaaatca cccctcttcc ggacggcggt ttcaaggtga tcgctcccag tgattcgctc
    12121 tttgaccccg ccaactccag ttcgctgaag gccgccgggc ccgactccgc cctgaatcaa
    12181 ctgttctcgc ttgcaaaatc ccaccccacc caccgtctcc tgctcaacgg ctatgccggt
    12241 tcagagaaaa cttcgacgag acgcgtggat ctgtccagcg cccaggtgtg ggcgatctac
    12301 agcgcgctgg tcaaaggcgg cgtctccgcc acgcgcctgg aggtgcacgg cctgggcgga
    12361 aaattccggg acgtcaaccg gatagaaatc ctcttcaccg cccctcccgc cccgccgccg
    12421 gacccccttc cggacgcccc cgcgccctga atccccctcc ccgcatgaca gccgacgccg
    12481 ggtcctccaa acccctcgtg ctcctgacgg gcgcgaccgg ctacatcggc ggttgcctcc
    12541 tctccctgct ggaacacgac cattttcccg tgcgctgcct ggtgcgccgg gaactcgttc
    12601 gccgcggttc cccggacacc acgagcaccg tgctcggcga cgtgctggat tcccaaagtc
    12661 tccggcgggc catggacggc gtgcacacgg cctactattt catccactcc ctcaaggcgc
    12721 gtgaaaattt cgaggcggag gaacggcggg gggcgcaaaa cttcgccttc gccgccaagg
    12781 ccgcgggcgt gcgccgcatc atctacctcg gcggactcgg cgatccgcac gacacccttt
    12841 ccccccactt gaagagccgc cgggccgtcg gcgacgtcct tcggtcctcc ggcgtcccgg
    12901 tcatcgagtt ccgcgcctcc atcgtcatcg gcgccggaag tctctcgttc gaactgatcc
    12961 gctgcctcgt acaacgcctg ccgatcatgc tcgccccccg ctgggttaca aacctcacgc
    13021 agccgatcag catccaggac cttctggcct acttccgcgc cgcgctggag ttgcccgagg
    13081 atggccatcg caccttcgag atcggcggcc gggacgtcgt ctcctacggc gacatcctcc
    13141 gggaatacgc ccgtcaacgg ggattaggac gcctgatcct tcccgtaccc ctgttgccgc
    13201 ggcccctctc ctgcctgtgg ctcaaactgg tggtgccgga atacgccgcc gtgggcgaaa
    13261 aactcttcga aagtctccgc aaccccaccg tcgtctccga ccacgccgcg acccgcgtct
    13321 tcggcctccg gccgaccggg ttccgcgaag cgctggcgac cgccctgagc cgggaagacg
    13381 actcgttccg ccggtcccgc tggggagaat cccccgccat caaggaagcg cccggtcgga
    13441 aaggcgtccg catcggttcc cgtatcctcg attgccggac ggtgcggttg gatgtcccgg
    13501 ccgcctccgc ctttgctccg atccagcgca tcggcgggac ccgcggatgg tatcacgccg
    13561 attggctctg gcaattccgg ggatttctcg acgaacttgt gggaggcgtg ggcatgcgcc
    13621 ggggacgtcg gcacccggaa gacttggccg tgggggacgt gctcgattgc tatcgcgtgg
    13681 aatacgtcga accggggcgg gaactcctgc tgcgggcgga actgcggctg ccggggcggg
    13741 cctggctgca gttcgaggtg tccggcgagg ggagctcttc cgagatctgc caatccgcca
    13801 tcttcgatcc caaagggctt ttcggattcc tctcctggta tgccctttat cccttccaca
    13861 aatacatatt ttccggaatg ctggaaaaaa tcgcggccgc cgcccgccgg gaacacgcga
    13921 gccgaacgca ttgatgtcgg ccctcgcccg cgccg
//
LOCUS       DAOTFD010000048        13783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3077_length_13783_cov_5.463942, whole genome shotgun sequence.
ACCESSION   DAOTFD010000048 DAOTFD010000000
VERSION     DAOTFD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_13783_cov_5.463942"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>13783
                     /locus_tag="P5079_04605"
     CDS             <1..>13783
                     /locus_tag="P5079_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY29302.1"
                     /translation="CLHHPKTHEMVKLLRDILNVAAPGVSLITETNVPHADNVGYLGD
                     GRDEAQMAYNFSLPPLVLHTFRTGDAEALTRWSRALAYPSDGGSFLNFLDSHDGIGLL
                     GARGLLSEEDIAALTQMVRSRGGLVSERTTDNGRKVPYELNTTLWDAINDPRRETDEG
                     LDLQTRRFLAARAIALLLRGVPAIYLHGLLGSSNDVEAARRTGVNRDVNRRNISQAEL
                     DDALRNPATRTFRVFTSLKKMLAARARHKAFHPQGKQTVLALNPGVFAVLRESPDGRE
                     KILGLVEVAGKTHRLPIDPEILGAEEGRPLTDAFARSAVDFKDGSVSLAPYQVLWLTP
                     RRGFRLTLRLAGQKLRDRLAAFVFRRSADAKTPADPPSLLADVHAGRQNVVYHDPASG
                     TAAHLVMHGGRAIAASPAGNAGIHFNVAGEGAAPRWIAEVRPTRDGDLRGFESAVEIS
                     RPDFELQDIYLGSVRSIRDRIRSHGGAVVDPRAARDMAELEKKGETIFATVVRHAVRA
                     DGSVLLELGGQTQGKAHLHRIRLVFPKGTAVEVIFFDGLDGPKECVKAVRVRGPPAET
                     LRFHTALLSDYPLPTTHRRWRDLFEPSALAYRDRLAREDAKWTAARIRRSTSPEDVRA
                     YKNLIADYQVSSSPERADGIRELEKRILKPSATSVLDLPVEKYHEELPFLKHATFEKM
                     LGTLRFLVTGEGVLAGSWQYLSYFGRDSLLVLRLLDQLLTPEAYKDLLSSVLRRVNEN
                     GEVAHEEDLRRSGESPERLDYRMHDGELMLLTLAQRFMDNPRFSHEEKRAFWETTGPE
                     RQTGATRTNGEILKRTAAYVLRKAEKLDADADTGWVGMKQVDGQTGVGDPTGNWRDAV
                     DSLARGMYAGDVNAVWMPAALKAVRDLQAADVFHLPETRLLHWIEVWDKASSSFRIYR
                     TKKDRADRFDRWLLDRLRRFPADAPLVFALLAQAMARKESETTEHLRALNDAWRSGGA
                     AAVAATFHRAASQNIWAARGLGYLALAKDLKGRNIAAVNGDYSTFAPLLRRLSLRELK
                     SVLRAILVPAALGGLRVRGVGVLSANPLLAGQHVSDPVRLRNGEPVRSVWDMLSFQSY
                     HGMVVWPAMVNIMLQGWIEQAEALLDARRRNDAQMLYAAIELVRADINSAGALKNHEV
                     LSLNAAEDGQLVAAPFSGEVESNYLQAWNAASEIAVQLSLDKLRRRLGAGGRAIRSQA
                     ETRNPRLEDDLAAWSRGEETATEEETAAPAASATPPRSPVASKSPLTLLARPFRPLVS
                     FFQKKKTPAPEEHPGAAAQVQTAATATADLLLDASLTPVQEAIPSVVKPALRIVVSAA
                     SEARKEIFNLPAMEEGHPTRAPPETLFVALEKEGIPHDVTAAFFWRAHALLYDEFAQG
                     GLERITFVKGSRFVAHADKDAHAVEVDIEFLKALGRLSEQDEYTVSGWLLAHELNHLR
                     ISGMSDAFEELGNVFLDTARLSERDRGVVRKFLENLKRDGHPTFGYERLLEPQRTDEQ
                     RMEAVVEFVWAAYPEYRDALTRMTLADLRKDAERLESLMGSLGANEKFFAARWVRAMT
                     AVTARQKSAEVRPLRDFSRWARVRQLFSVAPKTRKFLNNLAENLADGCEFKIENPRYI
                     VTLDSHGQDVYRFDVKLRMPGKKWKKTSLLVVADRAEGDAPRTPKMVALRPYSDTDGY
                     HLLLEGAPYTRVFEAVRAWLASYHGEELPIARDKFEGRELYDAVFFAPDRHDLENVLK
                     NPMLWHFLTVDELAGVLFRLDRLAVGGIDMGGFDQEGIMFMAPRYLNSAGHLSSTIGH
                     EKAHSLFDTLGKPTLRNRAVFYALETHFSNLRHKPLLQELAQDPTYAYAEFPKNPDSL
                     FSEMFAYTLETFLLLETKTLFKTPLLAEDLALLLELGYLPPWMNPGKFPQNVLPAEYV
                     QNAEITSDYYGFALWNYLLSTGDRNGWREMVRIAPEETREALKKTYARLGRAELQEAF
                     EELQNTVWPVEHVLLTGLRKDAAPDRAEWKKRAEEMAWAEWKSTYETDKKTELAPEKL
                     PLAKKYIIDQNSSRTHIIAREMEYLQEILKPLLRKLPPADIEGLAGHYAYVGKADPLL
                     RKLLLGYLDALTIAQYQKAGVITQRGKIVTWDGPTKKMVRELTKKIPLPAEILRPLDG
                     QGLYKRYKVRNLNDLLRATSARITHRIERQERRRGSARGLFPFWPQAWVQKYPKTLAA
                     VETVVLGGLAGGLSALLSHYTGWDLKGVLTGIGLLFSLVFSLIHYAVYYDIHAPPQSL
                     ADMTPGERWRVRGSLFALGLAMNATVAAGLLASPLVGGALGLLAAIGVHLLYNQDLAP
                     LLHLPLGILPKKEEPVFKSLSTEEFIEWVRQQPRTPPTDSARQPAAPKLKTVYHATDV
                     RLAGLIAEYGLYTPFLRATLTDADSKKYSESWVKEKTWESLVLLVFRVPPDWTGVSIA
                     AQGTVAHGSLGVRNKALLVDSLPDEMLQSIERLGLPQSKWLPQMLRDTLKEEKLARLP
                     AEFLDIDATLRENKHLLSDERYRELEKLLTEAKARAETKKPAAEAMLPAPVFGRPQEI
                     VWEWYRNDDLLRHEEAMAGLKLDAAYLKKRLDRLVEDDLRTRLPAAALKPLSSNTGYL
                     DVFDAPDSNFVVKIPNTVTLKDPKDLQKLLANYETAAERLGGLVPSMTILRDVTLTVN
                     GVPQSYPFVILQEKVTPLEKSAAYSTSREETFHQLAELTKALWARGVFDADFYRLAEN
                     YGLTADGRLVLFDLSNITTNIDRLVLFRESMTFFSAELYELYQFSEIEKYWEKDPAAG
                     RPAGPARLAGAKLQAAQKDVIARLTGAATASVQETVVSFDLSALQVLWQRGGEGFHRF
                     FAGLRARPDVRLVINTRADDVPGIERYLQETIRPSLLKTGSISADDAVVHDVLTVYPK
                     ELPGAAVKNKAEALRLWADDNKLDLSRTRFIHVDDSAHELRDIGFYAGPHGFAITTVG
                     LYPHNPAYREEIELSPAHHKIGIGNFAEKFGDILPPAAPSVTALHAGTVLRLVKMLIR
                     RGALAGVPFAKVLPLLGRSFSAGETRRLETIFESLARGASRGVLQELTRLVAPADLLR
                     VLLKLTLPRKTVPVKGEAEALRARLEKLFNAQKGDLQTAGLRRVTVKVHKGMDMIEGP
                     DSFILSPFLINADGTAEFHVNRLLLDALMKLDGKKRDEMLAALIEREISLREHITDGL
                     SFDVAHKKILRDPAQLELDRFARALIAGETVQPPSAESAPAQATTVPLEQIAQENLRT
                     FETLYEKFKAARERCPVTALPGKAEPVESLLADAPVFLEFKELAAADPRVRQSLGTHL
                     SNHLYLREGLSSLEKLLLLPGLVAATLNGDASLAGTSQKLFGHMHSLAETLRAFARES
                     SLDPAAAERVVSLAEYLDLQTWKAELSILAGILNTSELTRPAIEQHPAIQGAEIVPLD
                     ILPADYLWGQAPPQLLPPLEKRLRELSSLNALESVIFDETQTASFRLIEQVTGINRTY
                     RPGWLTRLNNAYKEVLDSKEDAPEFGDLRQFIRKELQQVSTGKINGLPSDLRVLQKIV
                     RGKIAELRAERLTGERRATTTAIRNFEHQVVDLIVAIIEHSMQHRTGLKGTFSLTQGL
                     AGRGNCWARADAFYALAKAFGVDAGKLIVFEMGGISGDFAYHGSNLVRSLTGGRPYVW
                     DVAFEGGLEPRALRGSDGSGRTLYFYGENKNEVLSGPSDDVWDAAMYEWLATHLLYEL
                     RGRRRAQSAGEESKIPRHFFSLANADSFVLQKIGALLQKALELDPESGSLLSAASDYY
                     FELGDYERALELITRAIEVQKPPFPTFYYNLSVTLARLGRWHEALKAMRQALTLRNDF
                     FRHIPFENRAGYMWETNYYDEEREQAAPFNDRMIQFALLRQLSDAKQISGVLNLRGIG
                     RPNGNAFLQRLIPFLLDRSGSAQPQLLAVIFWAVGQLENNGKAITAEEVAKMSARLPV
                     RLRSLVGTPSRKDTLAFLRRIETGIPLESVTALHAGKLPDLVKMLIHRRVLAGVPFAK
                     VLPLLGRSFSAGETRRLETIFESLARGASRGVLQELTRLVAPADLLRALLKLTLPHKT
                     VPVKGEAEALRARLEKLFNAQKGDLQAAEIRRVTVKVHEGTDMFEGPDTFILSPFLVN
                     ADGTAEFHVNRLLLDALMKMDGKKKTELLDALISREINLRKYILKDLPFDAAHKKILE
                     MDGQEELERFAGTLVSLAMLQDNPADPELWLHVGEMFEDQSDDITAAKAYLALLRLQP
                     ARPAPWAALGRILDANDELTAAKVAQQFAKQPPADGNLDAAFRLIKNSLSKPKLWEDM
                     GLGAFDTAFRAFLKASPEERPRLLPELIGRFKVFPNYFRKGHSFGPYEIVKPLGAGAM
                     GAVYKAVDSRTGKTVAIKINMQGTLNSVEHFLKEGRHLKRLHDLKTPAKVLFPAFVAM
                     GQDPQTRFPFIVMEYVDGSNLEKFIEARGPPKNSSDLEVSLRIAAQILQILQTLKQTR
                     LTHNDLKPDNVMLQTRTPSDRTRLDETYARAKLMDFGIAAPVGEELTMFFGPFGYIPP
                     EVARSYTPAAKSDIFAFGILLFHLLTGVHPVVFAGKELDDFDSTREIIQWTRRGVRDW
                     DAEAAGLPLSDLKKLIQKTLSQNPD"
BASE COUNT         2881 a         4621 c         3875 g         2406 t
ORIGIN      
        1 cttgtctgca tcatcccaag acccacgaga tggtcaagct tctgcgcgac atcctgaacg
       61 tggcggcgcc gggggtctcc ctcatcaccg agaccaacgt ccctcacgcg gacaacgtgg
      121 gctacctggg cgacggacgc gacgaggcgc agatggccta caatttttct ttgccgcctc
      181 tcgtgctgca caccttccgc accggtgacg ccgaggcgct cactcgatgg tcccgcgcgc
      241 tcgcctatcc ctccgacggc ggctctttcc tcaacttcct ggattcgcac gacgggatcg
      301 gcctgctggg agcgcgcggg ctcctctcgg aagaagacat cgccgccctc acccagatgg
      361 ttcgatcccg cggaggtctc gtctccgaac gcaccaccga caacggccga aaagtcccct
      421 atgaactcaa caccaccctc tgggacgcca tcaacgaccc tcgccgggag accgatgaag
      481 gcctcgatct gcaaacccgc cggttcctgg ccgcccgggc catcgccctc ctcctgcgcg
      541 gggtccccgc catctatctt cacggacttt tgggctcctc caacgacgtg gaggccgccc
      601 gccggacggg cgtgaaccgc gatgtcaacc gccgcaacat ttcacaggcc gagctggacg
      661 acgcccttcg gaacccggcg acgcgaacct tccgcgtttt tacttccctg aaaaaaatgc
      721 tggcggcgcg ggcgcgacac aaggcgttcc atccccaggg gaaacagacg gtcctggcgc
      781 tgaaccccgg cgtcttcgcc gtgctgcggg aatctcccga cggccgggaa aagatcttgg
      841 gactcgtcga ggtggccgga aaaacacacc ggctcccgat cgacccggaa attctggggg
      901 cggaggaagg ccggcccctg accgacgcct tcgcccgttc cgccgtcgat ttcaaggacg
      961 gctccgtttc cctggccccc taccaggtcc tgtggctgac gccccggcga ggattccgtc
     1021 tcaccctgcg gctcgccgga caaaaactca gggaccgcct ggccgccttc gtctttcgcc
     1081 ggtcggcaga cgccaaaacg ccggcggacc cgccgagcct cctggcagac gtccacgccg
     1141 gccgccagaa tgtggtctac catgaccccg cttccggaac ggcggcccac ctggtgatgc
     1201 acggcgggcg ggccatcgcg gcctcgccgg ccggcaacgc gggaatccat ttcaacgtcg
     1261 ccggagaagg cgcggccccg cgttggatcg ccgaggtccg accgacaagg gacggcgacc
     1321 tccggggatt cgaaagcgcg gtggaaatct cgcgccccga tttcgaactc caggatatct
     1381 acctggggag cgtccgctcc atccgggacc gcatccgttc ccacggcggc gcggtggtgg
     1441 acccacgcgc cgcaagggac atggccgaac tggagaaaaa gggagaaacg atcttcgcca
     1501 ccgttgtccg ccacgccgtc cgggcagacg ggagcgtcct gttggagctg ggcggtcaaa
     1561 cgcagggaaa agcgcacctc caccggatac gtctcgtttt cccgaaagga acagccgtcg
     1621 aagtgatctt tttcgacggg ctggacggcc ccaaggaatg cgtcaaagcc gtccgggtgc
     1681 gtggcccgcc cgccgagacg cttcgtttcc ataccgccct gttgtcggac tatcccctcc
     1741 cgacgaccca ccgacgatgg cgggaccttt ttgaaccatc cgccctggcc taccgcgacc
     1801 ggctggcccg cgaagacgcg aaatggaccg cggcgcggat ccgacgatcc acttctccgg
     1861 aagatgtccg cgcctacaaa aacctcatcg ccgattatca ggtctcctcc tctcccgaac
     1921 gtgcggacgg aatacgcgaa ctggaaaaac ggattttgaa gccgagcgcc acgtccgtgt
     1981 tggacctccc cgtggaaaaa tatcacgagg aactgccctt tctgaaacac gccacctttg
     2041 aaaaaatgct cggaaccctc cggttcctcg ttaccggcga aggcgttctg gccgggtcat
     2101 ggcagtacct gagttatttc ggaagagact ctctcctcgt gctccgcctg ctggaccagt
     2161 tgctcacacc ggaggcatat aaagaccttc tgtcctccgt tctccggcga gtcaacgaaa
     2221 acggcgaggt cgcccacgag gaagacctgc gccgctccgg ggaatccccg gaacgcctgg
     2281 attaccgcat gcacgacggc gaactgatgc tcctgacgct ggctcagcga ttcatggaca
     2341 acccgcgttt cagtcatgag gaaaaacgcg ctttctggga aacgaccggc ccggaacgcc
     2401 aaaccggcgc gacccggacc aacggcgaaa tcttgaagcg gaccgccgcc tacgttctga
     2461 gaaaagccga aaaactggac gccgacgccg acaccggctg ggtggggatg aaacaagtcg
     2521 acggacaaac cggggtcggc gatcccaccg gaaactggcg cgacgcggtg gattccctcg
     2581 ctcgcggcat gtacgcgggg gacgtgaacg cggtgtggat gccggccgcc ctgaaagccg
     2641 tccgcgacct gcaagccgcg gatgttttcc atctgccgga gacacggctt ctccattgga
     2701 tcgaggtctg ggacaaggct tcttcctctt tccgaattta ccgaacaaaa aaagaccggg
     2761 cggatcgttt cgaccgatgg ctcctggacc gcctacgccg gttcccggcg gacgcgcctc
     2821 ttgttttcgc cctgttggct caggcaatgg cccgcaaaga atctgaaacc acggagcacc
     2881 ttcgggccct gaacgacgcc tggcggagcg gtggggcggc ggcggtcgcg gcaaccttcc
     2941 accgcgccgc ctcccaaaac atctgggccg cccggggcct gggatacctc gcgctggcca
     3001 aggacctgaa aggccggaac atcgcggcgg tcaacgggga ctactccacc ttcgcccctc
     3061 ttttaagacg cctttccctg cgcgaactga aaagcgtcct gcgggcgatc ctcgtcccgg
     3121 ccgctttggg tggcctgcgc gttcggggcg tgggcgtgct gagcgccaat cccctccttg
     3181 ccggtcaaca cgtttccgac cccgtccggc tccggaacgg agagcccgtc cgttccgtct
     3241 gggacatgct ctctttccag tcctatcacg ggatggtcgt ctggcccgcc atggtcaaca
     3301 tcatgctcca aggatggatc gaacaggccg aggcgctcct ggacgcccgg cgtcgaaacg
     3361 acgcgcaaat gctttacgcc gccattgagc tcgtccgcgc ggacatcaac agcgccggcg
     3421 cgctgaagaa ccacgaagtc ctctccctca acgccgccga agacggacaa ctcgtcgccg
     3481 cgcccttttc gggcgaagtc gaatccaatt acctgcaggc ctggaacgcc gcctccgaaa
     3541 tcgccgtgca actgagtttg gataaactgc gccggcggct cggcgcgggc ggacgagcca
     3601 ttcgttccca ggcggaaacc cgaaacccgc ggctcgagga cgatctcgcc gcctggagcc
     3661 gtggagaaga aacagcaact gaggaagaaa cggcagcccc ggcggcctcc gcgacgcccc
     3721 cccgatcccc cgtcgcctcg aaatcccccc tcactctttt ggcccggccc ttccggcctc
     3781 tcgtatcctt cttccagaag aaaaaaacac cggctccgga agagcacccc ggcgcggccg
     3841 cccaggtgca aaccgccgcg acggccaccg ccgacctgct gctcgacgca tccctgacgc
     3901 ccgtccaaga agccatcccc tccgtcgtca aaccggccct gcggatcgtc gtcagcgccg
     3961 cctccgaggc gcggaaagaa atcttcaatc tgcccgccat ggaagagggc catcccaccc
     4021 gcgccccgcc cgaaacgctt tttgtggcgc ttgagaaaga agggattccc cacgacgtga
     4081 ccgccgcgtt cttctggcgg gcccacgccc tcctctacga cgagttcgcg cagggcggcc
     4141 tggaacggat cacgttcgtg aaaggcagcc gcttcgtggc ccacgccgac aaagacgctc
     4201 acgccgtgga agtggacatc gagttcctca aggccctggg aaggctctcc gaacaagacg
     4261 aatacaccgt ttcgggatgg ctgctggccc atgaattgaa ccacctccgt atcagcggca
     4321 tgagcgacgc gttcgaggag ctgggaaacg tctttctgga tacggcccgg ctgtccgaaa
     4381 gagaccgcgg cgtcgtacgg aaattcctcg aaaatctgaa gcgggacggc caccccacct
     4441 tcggctacga acgcctcctc gagccgcaac ggaccgatga acaacggatg gaagccgttg
     4501 tggagttcgt ctgggccgcc tatccggagt acagagacgc cctgacccgg atgacactgg
     4561 cggacctccg gaaagacgcc gaacgcctgg aatccttgat gggaagcctg ggagcgaacg
     4621 aaaagttttt cgccgcccgc tgggtccgcg ccatgacggc cgtgaccgca cgccagaaaa
     4681 gcgcggaggt ccgcccgctc agggatttct cccgctgggc gcgcgtccgt caactgttct
     4741 ccgtcgcgcc caagacccga aaattcttga acaacctggc ggagaatctg gcggatggtt
     4801 gcgaattcaa aattgagaat ccccgctaca tcgtgaccct ggactcgcac ggccaggacg
     4861 tgtaccgatt cgacgtcaag ttgcggatgc ccgggaagaa gtggaaaaag acgtccctcc
     4921 ttgtcgtcgc cgatcgcgca gagggagacg cgccgcgaac gcccaagatg gtggccctcc
     4981 ggccctattc cgacaccgac ggctaccacc tactcctcga aggcgccccg tacacccgcg
     5041 tctttgaagc cgtccgcgcg tggctcgcct cctatcatgg ggaggaattg cccatcgccc
     5101 gcgacaaatt cgaaggccgc gagctctacg acgccgtctt cttcgccccc gaccgccatg
     5161 acctggaaaa cgttttaaag aaccctatgc tgtggcactt cttgaccgtc gacgagctgg
     5221 ccggtgtcct cttccgattg gacagactgg ccgtcggcgg catcgacatg ggcggattcg
     5281 accaggaagg catcatgttc atggccccgc gctatttgaa cagcgccgga cacctctcct
     5341 ccaccatcgg gcacgaaaaa gcccactccc tcttcgacac actcggcaag ccgacgctcc
     5401 gcaatcgcgc cgtgttctac gccctggaaa cccatttcag caacctgcgg cacaaaccgc
     5461 tcctccaaga attggcccag gaccccactt acgcgtacgc cgaatttcca aaaaatccgg
     5521 actcgctgtt cagcgagatg ttcgcctaca cccttgagac gtttctcctg ttggaaacaa
     5581 aaaccctttt caagacgccg cttttggcgg aagacctggc cttgcttttg gaattgggat
     5641 acctgccgcc ctggatgaac cccggaaaat tcccccagaa cgtcctcccg gcggaatatg
     5701 tgcagaatgc ggagatcact tcggactact acggttttgc cctctggaac taccttctgt
     5761 ccacgggaga tcggaacggc tggcgggaga tggtgcgcat cgcccccgag gaaacgcggg
     5821 aggcgttgaa aaaaacatac gcgcggctcg gccgggccga actccaggaa gccttcgagg
     5881 aactccagaa caccgtctgg ccggtggaac acgtcctctt gacgggcctt cgaaaagacg
     5941 ccgcccccga ccgcgccgaa tggaaaaaac gcgcggaaga aatggcctgg gcagagtgga
     6001 aaagcaccta cgaaaccgac aagaaaaccg agttggcccc cgaaaagctg cccctggcga
     6061 aaaaatacat catcgaccag aacagttccc gaacccacat catcgcccgg gaaatggaat
     6121 acctccagga aatcctcaaa ccgctgttgc ggaaattgcc gccggccgac atcgaagggc
     6181 tggcgggcca ttacgcctac gtcggcaaag cggatcccct cctgcgcaaa ttgctactgg
     6241 gctatctcga tgccctcacc atcgcgcaat accaaaaagc cggggtcatc acacagcgtg
     6301 gcaagatcgt gacctgggac ggccccacca aaaagatggt gagggaattg acgaaaaaga
     6361 tccccctccc ggccgagatc ctccggccgc tggacgggca aggcctttat aaacgctata
     6421 aggtccggaa cctcaacgac ctccttcgcg cgacgagtgc gagaatcacg catcgcattg
     6481 aacggcagga acggagacgc ggcagcgccc gcggactttt ccccttctgg ccacaagctt
     6541 gggtgcagaa atatccgaaa accctggccg ccgtcgaaac cgtggtgttg ggtggactag
     6601 cgggaggcct ctcggccctc ctgagccatt acaccggctg ggatttgaaa ggagtgctga
     6661 ccgggatcgg gctccttttc tccctcgttt tctcgctgat ccattacgcg gtctactacg
     6721 atatccacgc gcctccccag tcgctagccg acatgacgcc cggggaacga tggcgcgtgc
     6781 gcgggtctct cttcgccttg gggctggcca tgaacgccac cgtggcggcg ggccttttgg
     6841 cgtcccccct tgtcggcggt gccctcggcc tgctggcggc catcggcgtg caccttctct
     6901 acaaccagga cctggccccc ctccttcatc ttccgctcgg catcctcccc aaaaaagagg
     6961 agcccgtctt taaatcgctt tcgacggaag aattcatcga gtgggtccgc caacaaccgc
     7021 gaaccccgcc gacggacagc gcccgccaac cggccgcgcc gaaactgaaa accgtgtatc
     7081 acgccaccga cgtccgcctg gccggcctca tcgccgaata cggcctctac acccccttct
     7141 tgcgggccac tctgaccgat gcggattcaa aaaaatattc cgaaagctgg gtgaaagaaa
     7201 aaacctggga atccctcgtg ctcctggtct tccgggtccc tcccgattgg accggcgttt
     7261 ccattgcggc gcagggcacg gtcgctcacg gttccttggg agtgcgcaac aaagccctcc
     7321 tggtcgattc tcttccggac gaaatgcttc agtccattga gcggctgggt ctgcctcaaa
     7381 gcaaatggct tcctcagatg ctgcgcgaca ccctcaagga agaaaaactg gcgcgcctcc
     7441 cggctgaatt tttggacatc gatgccaccc tgcgggagaa caaacacctc ctttcagatg
     7501 aacgctaccg tgaactggag aaactattga ccgaggcaaa ggccagggcg gaaacgaaaa
     7561 aacccgccgc cgaggccatg cttccggccc ctgttttcgg acgaccgcag gaaattgttt
     7621 gggaatggta tcggaacgac gatcttctgc ggcacgaaga agcgatggcc ggcctcaaac
     7681 tggacgccgc ctatctgaaa aaacgcctgg accggctcgt ggaagacgac ctgcggacgc
     7741 gcctgccggc cgccgcgctg aaacctttaa gctccaacac gggctatctg gacgtgttcg
     7801 acgctcccga ttccaatttc gtggtgaaga tccccaacac ggtcacgctg aaagacccga
     7861 aggacctaca gaaactgttg gccaattacg aaacggcggc ggaacgcctc ggcgggctcg
     7921 ttccgtccat gaccatcctc cgggacgtga cgctgaccgt taacggcgtc ccccaatcct
     7981 atcccttcgt cattcttcag gaaaaagtca ccccgctgga aaaaagcgcg gcgtattcga
     8041 cctctcggga ggagaccttc caccagctcg ccgagctgac gaaggccctg tgggcccgcg
     8101 gcgtgttcga cgcggacttt taccgcctgg cggagaacta cgggctcacc gccgacggcc
     8161 gcctcgtcct gttcgacctc agcaacataa caacgaacat cgaccgactc gtcctcttcc
     8221 gggaatcgat gacgttcttc agcgcggaac tctacgagct ctaccagttc agcgaaatcg
     8281 aaaaatattg ggaaaaagat ccggccgccg gacggccggc aggccccgcg aggctggccg
     8341 gcgccaagct ccaggcggcc cagaaagatg tgatcgcccg gctcaccggc gcggcgaccg
     8401 catccgttca ggaaacggtc gtgtcgttcg acttgtccgc gttgcaggtc ctttggcagc
     8461 gcggcggtga aggattccat cgcttcttcg ccggcctccg cgcccggccg gacgtgcgcc
     8521 tcgtcatcaa cacccgggcc gacgacgtcc cggggatcga acggtatctc caagaaacca
     8581 tccgcccctc tcttttaaaa accggaagca tctcggccga cgatgccgtc gtacacgacg
     8641 ttctgacggt ctaccccaaa gaattgccgg gggccgcggt gaagaacaag gcggaagctc
     8701 tccgtctctg ggccgacgac aacaaattgg acctgtcccg cacccgcttc atccacgtgg
     8761 acgactcggc gcacgaattg cgcgacatcg gcttctacgc cgggcctcac ggtttcgcca
     8821 tcaccacggt cggactctac ccccacaacc ccgcctaccg cgaggaaatc gaactgtccc
     8881 cggcacacca taaaatcggt atcgggaact tcgcggaaaa attcggcgac atcctgcccc
     8941 ccgccgcccc ctccgtcacc gccctgcacg ccggaaccgt tctgcgtctg gtgaaaatgc
     9001 tcatccgccg gggcgcgctg gccggcgtgc cgttcgccaa ggttctcccc ctcctgggcc
     9061 gatccttctc ggcaggggaa acccgacgac tggaaacgat ctttgaatcc ctggcccgcg
     9121 gcgcctcccg aggcgtgctg caggaactga cccgcctggt ggccccggcc gaccttctcc
     9181 gggtcctgtt gaaactgacg ttgccgcgca aaaccgttcc ggtcaagggc gaggcggaag
     9241 ccttgcgcgc gagattggaa aaactgttca acgcacagaa gggagacctt caaacggcgg
     9301 ggctccgccg ggtcacggtc aaagtccaca aaggcatgga catgatcgag gggccggatt
     9361 ccttcatcct ctcaccgttc ctgatcaacg ccgacggaac ggcggagttc cacgtcaacc
     9421 ggctgctgct ggacgccctc atgaaactgg acggcaaaaa aagggacgaa atgctggcgg
     9481 ccctgatcga acgcgagatt tccctccgcg aacatatcac cgacggcctc tccttcgacg
     9541 tcgcccacaa aaaaatcctc cgcgaccccg cccaactgga actggatcgg ttcgcgcgag
     9601 cgctgatcgc cggcgaaacc gtgcagcctc cttcggccga gtccgcgccg gcccaagcga
     9661 cgacggtccc tcttgaacaa atcgcgcaag aaaacctccg cacttttgag accctctatg
     9721 aaaaattcaa ggccgcgcgg gaaagatgtc cggtcaccgc cctccccggc aaggccgaac
     9781 cagtcgaatc gctgctggcg gacgcccccg ttttcctgga gttcaaggaa ttggccgccg
     9841 ccgatccgcg agttcgacag tccctcggca cgcacctcag caatcacctc tacctgcgcg
     9901 aggggttgtc ttcgctggaa aaactccttc ttctgcccgg actcgtcgct gccacgctga
     9961 acggcgacgc gagtttggcc ggaacgagcc aaaaactctt cgggcatatg cattccctcg
    10021 ccgaaacgct tcgcgccttc gcgcgggaaa gcagcctcga cccggccgcg gccgagaggg
    10081 ttgtttccct ggcggaatat cttgacctgc agacctggaa ggcagaactg agcatactgg
    10141 ccggcatttt gaatacaagc gagctgactc ggccagccat tgaacaacac cccgcgatcc
    10201 agggggcgga gatcgtaccc ctcgacatcc tgcccgccga ctatctttgg ggccaggcac
    10261 cgccgcaact tttgccgccc cttgaaaaac gtctgcggga actgtcctct ctcaacgccc
    10321 tcgaatccgt gatcttcgac gaaactcaaa cggcaagttt ccgcctgatt gaacaggtga
    10381 ccgggatcaa caggacctac agacccggat ggctgacccg gctgaacaac gcctacaagg
    10441 aagttctgga ctcgaaagag gatgcccccg agttcggcga tctgcggcaa ttcatccgca
    10501 aagagctcca acaggtgtcc acgggaaaaa tcaacggcct cccctcggac ctccgtgtcc
    10561 tccaaaaaat cgtgcgcgga aaaattgccg aattgcgcgc cgagcgcctc accggcgaac
    10621 ggcgggccac gacgaccgcc atacgcaact tcgagcacca ggtcgtggac ctgatcgtgg
    10681 ccatcatcga acattcgatg caacatcgaa cgggcctgaa agggacgttc agcctcaccc
    10741 agggcctggc cggccgcgga aattgctggg cccgcgcgga cgctttttac gcactggcga
    10801 aagcctttgg cgtggacgcg gggaaactca tcgtttttga gatgggaggc atcagcgggg
    10861 atttcgccta tcacggcagc aacctcgtgc gcagcctcac cggcgggaga ccctatgtat
    10921 gggacgtggc tttcgaaggc ggtctggaac cgcgcgccct gcgcggcagc gacggttcgg
    10981 gccggacttt gtatttttac ggggaaaaca aaaacgaggt gctcagcggc ccctccgacg
    11041 acgtatggga cgccgcgatg tacgaatggc tggcgaccca tcttctctac gaactccgcg
    11101 ggcggcgcag agcccagtcc gccggggagg agtcgaaaat ccccagacac ttcttttccc
    11161 tcgccaacgc cgactccttc gtattgcaaa agatcggcgc cctgctccag aaagcgctgg
    11221 aactcgatcc cgaatcgggc tcgttgctct ccgccgcgag cgattattat ttcgagctcg
    11281 gggactatga gcgggcgctg gaactcatca cgcgggccat cgaagtccaa aaacccccgt
    11341 tccccacgtt ctactacaat ctctccgtca cgctggccag actgggccgc tggcacgaag
    11401 ccctcaaggc aatgcgccaa gctttgaccc tccggaacga cttcttccgg catatcccgt
    11461 tcgaaaatcg cgccggctac atgtgggaaa cgaattacta cgacgaagaa cgggaacagg
    11521 ctgccccctt caacgaccgg atgatccagt tcgccctcct cagacaactc tccgacgcga
    11581 aacagatcag tggtgttttg aaccttcggg gaatcggccg gcccaacgga aatgcgttcc
    11641 tacagcgcct cattcctttc ctactggaca gatcgggttc ggcccaacct caactcttgg
    11701 ccgtgatatt ttgggcagtg ggccaattgg aaaacaacgg aaaagctatc accgccgagg
    11761 aagtggcgaa aatgtcggcg cggcttcccg tccggcttcg ctcgttggtg ggaactcctt
    11821 cacgaaaaga caccttggca tttttgcgcc gtattgaaac cggcattccg ttggaatccg
    11881 tcaccgcgct gcacgccggc aaactcccgg acctggtgaa aatgctgatc caccggcgcg
    11941 tcctggccgg cgtgccgttc gccaaagttc tccccctcct gggccgatcc ttctcggcag
    12001 gggaaacccg acgactggaa acgatctttg aatccctggc ccgcggcgcc tcccgaggcg
    12061 tgctgcagga actgacccgc ctggtggccc cggccgacct tctccgagcc ctgttgaaac
    12121 tgacgttgcc tcacaaaacc gttccggtca agggcgaggc ggaagccttg cgcgcgagac
    12181 tggaaaaact gttcaacgca cagaaggggg accttcaagc ggcggagatc cgccgggtca
    12241 cggtcaaagt tcacgagggc acggacatgt tcgaggggcc ggacaccttc atcctttccc
    12301 ccttcctggt caacgccgac ggaacggcgg aattccacgt caaccggctg ttgctggatg
    12361 cgctgatgaa gatggacggc aagaaaaaaa cggaactctt ggacgccctg atctcccgcg
    12421 aaatcaacct ccgcaaatac attttgaaag acctcccctt cgacgccgct cacaagaaaa
    12481 ttttggaaat ggacgggcag gaagaactcg agcggtttgc cgggacgctg gtgtccctgg
    12541 ccatgctcca ggacaacccg gccgatccgg aattatggct gcatgtcggc gagatgttcg
    12601 aagaccagtc cgacgacatc accgccgcca aggcctattt ggccctgctt cgactgcagc
    12661 cggcccgccc ggcgccctgg gcggccttgg gacggatact ggacgcgaac gacgagctca
    12721 cggcggcgaa ggtggctcaa caattcgcga aacaaccgcc ggccgacggc aatctggacg
    12781 cggctttccg gctcatcaaa aactcgctgt ccaaaccgaa actctgggaa gacatggggc
    12841 tcggcgcttt cgacaccgcg tttcgcgcct tcctcaaagc gtccccggaa gaacgccccc
    12901 gtttattgcc cgaactgatc ggccggttca aagtattccc caattatttc cgcaaaggcc
    12961 attccttcgg cccctatgag atcgtcaaac cgctgggagc cggcgccatg ggcgccgtgt
    13021 acaaagccgt cgactcgcgc accggaaaaa cggtggctat taaaataaac atgcagggga
    13081 cattaaattc cgtggaacat tttctgaaag agggccggca tctcaaacgc ctgcacgatc
    13141 tcaaaacacc cgccaaagtt ctgttccccg ccttcgtcgc catggggcag gatccacaaa
    13201 cgcgctttcc cttcatcgtc atggaatacg tggacggatc gaatctggaa aaattcatcg
    13261 aggcccgggg gccgccgaaa aactcctccg acctggaagt ttccctgcgg atcgcggccc
    13321 agatccttca aatcctccaa acgttgaaac aaacccggct gacacataac gatttaaaac
    13381 ccgacaatgt gatgctgcaa acgcgaaccc cctcggaccg aacgcggctg gacgaaacgt
    13441 atgcccgggc gaaactgatg gactttggaa tcgccgcccc cgtcggcgaa gagctgacga
    13501 tgtttttcgg ccccttcggg tatattcccc ccgaagtcgc ccgatcctac acccccgccg
    13561 caaaatcgga catctttgct tttggaatcc ttcttttcca cctcttgacc ggggtccatc
    13621 cggtggtttt cgccggtaaa gaactggatg actttgacag cacccgggaa atcattcaat
    13681 ggacccgccg gggcgtgcgg gattgggacg ccgaggccgc cggcttgccg ttgtctgact
    13741 taaaaaaact catccaaaaa accctttccc aaaaccccga cga
//
LOCUS       DAOTFD010000049        13604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3134_length_13604_cov_3.973651, whole genome shotgun sequence.
ACCESSION   DAOTFD010000049 DAOTFD010000000
VERSION     DAOTFD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3134_length_13604_cov_3.973651"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="P5079_04610"
     CDS             complement(<1..1234)
                     /locus_tag="P5079_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29303.1"
                     /translation="MTDGLSFIRRRLRRARFWKAGYAVLLIFQKTLVFFGASGMSIFL
                     ADRLFPLSPAVRMGAWLVLTAGSLALAAANGVRPRQWRSSAVAAELGSPLHPADCVLN
                     AWEMGRGDIAKRAVSPELVEREVERVCQRLRPLSVGKTMRPGNWRRWTALATATSLLV
                     SGLFLLLPENARASLWGAYCPFALRENLAGVSVDPGDARVPVGGDVPIRVTLRTPSVS
                     PELWLKRGATWQKRDLFKTAERDFSHTLKNLLEPVSYKVRHQGRWSRVYRIEPFRPPR
                     FERFRVDIVYPPYTRKPRERQENVMTTRVLRGAALTARGRLDRKVDDLAVTSPDGRVW
                     SATLNDGDALEMSLTAHESAELHFALKSGAARDELVWRIEVEDDRAPHVQILSPAQDL
                     LLSANEKLPMTFTATDDFG"
     gene            complement(1231..3012)
                     /locus_tag="P5079_04615"
     CDS             complement(1231..3012)
                     /locus_tag="P5079_04615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29304.1"
                     /translation="MTNPKKNIEARRTPASPPPVFSRRAAVLWLPLLVLGIGVHVCSG
                     RLAVRAQSPTFDEPLHLTAGFVYWKTRDYRLNAYHHPPLSSLVGAVPLLFMGPALPQD
                     DPLWLQPRWGDPTRQYAFANRFLYHNASGLDADRMVNAGRYAMLFCSAVCLVGFFFAV
                     RSVFGEVAASVAFLLAAVSPEFIAHGSLVTTDYLFTWFYFFFFYFLDRWDRSTEEAPG
                     PGPRAFLWAGLTGMSLVLAFCSKFSAVAVGPAVLLWLFHRKFRPGLSFRGMLVCFATA
                     AVSLLLVYQVTALPVFFGGLRYTLERLQGGRATFLLGNHSTEGWWFYFPTVFFLKTAT
                     PVLVGFLFSLGLALRKKVRWPWLVVTPPLVYFLAACASSVQIGHRHILPVYPFLFVML
                     GIAAAALWAHRPARYGLWALGVWLLAETFAARPWFLSYFNSLTAARVAGYEYLTDSNV
                     DWGQGMRALRQYLTEQKVGRIYLSYFGTAEPASYGISYAAVAPYTLPAFADQDVDLRA
                     EPRALLVVSATNLQSTYFADKDIFDWLKNRAPEKVLAGSLLVYDVTRDALSHQRLAGL
                     FGRMGKPEMVRRESLWAVDLLGGTDKT"
     gene            complement(3236..5425)
                     /locus_tag="P5079_04620"
     CDS             complement(3236..5425)
                     /locus_tag="P5079_04620"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29305.1"
                     /translation="MNGADRLPGQDDFGARTRRVLPFVLFLAFWGFHLHFAGGTVVPY
                     RDSGEMVTDAHTLGVAHPPGYPLYVLLGKVFVQSGWGNPAYRVNLLSSLSSAAAETGL
                     FLLMWGVTGSAAVAGAAALWATSAIFWELSSVSEMYTLGLLGMVLLFVFVRRISDEHV
                     PRPSRWWPAAAFLFGVGCGVRTDFLLLSPALAYLCWRRGGWRILFPALGAAFLGLTVF
                     LYLPLRSARQPWVDWNNPEGLDRFLGSLMRKSHGGTLDLLSKSYALGENFVSEFILYL
                     KTLASDFTWVGLPLAVLGGGHLRKRLPVFSTFLLINLAVFGPLFLFLANMPPNPHAVA
                     IVEAHYAVSHLIVALLMAAGLAAVFRWRGGGKRTTLLLTAAVSALVAVNAAAHRGRGD
                     KRWNLYARDYVGNMLRSSLPGSFGVLREDVPLFAFWERQLVSHQRPDVTIVAQGLAAS
                     PWYQETMKQAGRFVPLGRLDDERGWESYVNALAGKPLWVGGDANLSWKNETKPAGLVI
                     YLTWPAEVGPVLFDPLRMFEFYVIRGERRQTRAPDFFSSDLLTEYARAALRASSALMR
                     EKRWDEAALSLRRGMVFDSEFPALPFNWGYVLYQQGRLEESGAADARASRIFEELFRR
                     AAYYKSLPAVVEGLRREAAEVWIHRGVLAEKRGDLEAARNSYAQAVRLDPKSSQAHYN
                     WAVTYWNREWDQAVAHMQAAVAADPGNAQAAYFLDKARALREQALKK"
     gene            complement(5422..7533)
                     /locus_tag="P5079_04625"
     CDS             complement(5422..7533)
                     /locus_tag="P5079_04625"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29306.1"
                     /translation="MTDVKRFFMETPPGWRWAAPSVLFLVLWFFYVRGAQPCLAPGDS
                     AELTAAAATLGVAHSPGYPFYVLLGNVFGHVVPWGNPAFRMNLFSGFSTALALALLAC
                     LWRSAPAPAHAVLLLAGLSPLFLKLSTAAEVFGLNVLAVGVLALALGKFCETVSSRWF
                     LLSLFLFGLWLGNQQTLILVFPFLFLWGVGRLRVSGGLKGGTAFFRFLAAVGVFFFLG
                     LTVYVFLYLRSRSQPLLDWEDPETWDRFWKVLSRARYGTLQLAQGTPPPHDWNALWTQ
                     LRFFGDKTLENWRWGGLALLLTGGTVWMRRGIREGGWARQETVWFLGALACGPLFLFW
                     ANIRPEAATREMLERFLFLPLFMLVFVGVEGFLFLWRTGRWGKIVGALCLFQWTAVVF
                     ANGSLPSDRWDLAVGDHGRNVLRTAPSGAYLFADRADETEFSLAYLMDVAFKRPDVDF
                     VDANAGVSRSLYGKDYYEVWGKPRLARRQRVEGGMLASTSRPFYYATLDVSQLALPRR
                     AAGLLFEASPSASFSSRTAFPYWDVYLWRWPDGPMGARELHLAASTSRLMGDYAFALN
                     DLRQAERFIRMGEKAGMSPWDFYLATWHHRQGHWAEAERLYRRLAGRPGSEAVAWNNL
                     GVLCSDQNKFQEALRYYRKAIERDPRYAEAYYNSGVVYWRLGDWRAVMENLEQVLRWN
                     PAHPEARKFLLQARRRLSGGS"
     gene            complement(7520..9619)
                     /locus_tag="P5079_04630"
     CDS             complement(7520..9619)
                     /locus_tag="P5079_04630"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRY29307.1"
                     /translation="MGEDLTRRSRWERTVGAVLLAGLWACFLRHAAPSVTVGDSGEFI
                     TAAATLSLPHSPGYPLFALIGKLFSSLFPWANAGYRLNISSAALNLVSVGLVFFIATR
                     RGGLSWRGGAFCGLLLAGSPAFFLNGLVTEVFALNTALALAVAALFLAGGPRAAVVGA
                     FLFGLGLGNHHTLLLTAPALFLFCFSSSSGSPERGEERLRRVGRLLLPMGLFALLGFS
                     VYAFLPLRSRAQPPLDWGNPETLNGLLRTVSRKDYGSFSLALGETPPRTLATAAKQMR
                     RFGKVSVRQLSPWGAVAAVLGFFLLWRSVRWTALGLFAAWFFTGPFFMWLGNLPFDAQ
                     SDGIMDRFYILPTVFGVLAAGGFWKFAEARLKRWAWPLLAFPLFQLWSVGREFPLRGD
                     FLVQDYSQNILRTLPPGAVFFMDGGDDTFYSMAYQRFVRRQRDDLAVFDRGGLIFSSA
                     YGPDFRSLPREAKDRRRQDVERNWLTRRPLYYSTMNDRLLPGVPLGLTGFLYRAGASD
                     GNAGLCDLYAFRSLYPPPSQDYRTLALAPYFPFMASRASFQERQYARAATLMERTRRM
                     GGRVPWLQENLMGQCLEFGYDRLQAGDFEAAGLFYDVALRMKQPKSELYSNLGVLWER
                     RGNPAEAVRSYENALRLSPDNVDALYNLAVVFWRRSDWARVVPLLERVIALNPQHPGG
                     RYLAAARERRAGRHDGR"
     gene            complement(9604..11814)
                     /locus_tag="P5079_04635"
     CDS             complement(9604..11814)
                     /locus_tag="P5079_04635"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRY29308.1"
                     /translation="MNDLLVHLRALTAVEHLVWLALLAALSVFALLTWRGRVSGRRSL
                     LLIGVRAVEGILLTAIFFQPVVERRVRVERVPVAIVVDHSESMAVRDAGEAGDVSRWN
                     EALSKLELTRPDWERLCRPVYYVLGSRLKEAPGGVLSAVRPDGPASDFARIGEIPRKA
                     PSVRAVFFFSDGRAGGGRDPAAAFSDLGIPVYAIGVGKPETAPDVLLASLRAPHFAFK
                     NTDVEVVVRVEKRNITVDRLTVQVLRGDESVAAKTVEFAGGDAAETVLVFRPDSVGLQ
                     TYSVRLPVYEKESNRKNNSQAFTLDVSRDRVRVLYISGQPGPHYAFLRHQLKTNPSVE
                     LVSFVILRDPEDAVGVPDNQLALIPFPTQDVLVGQMNSFDIIVLEQFAFSSFGLGPSA
                     MAALRDYVEKGGGLLLIGNARMLGPAGPYVGTPVAEVLPFALDSPLRAGPQRYILESY
                     APDHPLMAIEENPEAARALWARMPALDGDGQFLAKTRPGAVVVAGCPAADGRLPVLAA
                     WTRGRGRAAMFLSLTSWRWALEEAGRGQGAAGYQKFWNNLLRWLSASREFRLVRLSVP
                     NEPVSLGEEIFLKAFVRDENYRPMNSAQVRAVITDPAGAARQRDLAFLGKGEYADAFV
                     PEKDGLYRVSVRAFSNGRKLGEDSGVVRCARLWVENRDTSTDFPSLTRWASAGGGEFV
                     PAAGFSSEWLEKNVSSSAWSFERREAVWNAKGIFAVLILLLFGEWLGRRRWGKI"
     gene            complement(11811..12548)
                     /locus_tag="P5079_04640"
     CDS             complement(11811..12548)
                     /locus_tag="P5079_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRY29309.1"
                     /translation="MRRLMLAGLMFFASTVFSAEGGGPENRFVYAQLRWQGTWDPYPA
                     LWPQIGRYLQQTTSVVPWPERRTLTVDDPALFESPFLVILGQGAVSFSEAEKERLRRY
                     IIGGGMIFLDNTEAERNSPFGRSAPALLKSLFPESTWEALPSDNVVFRSFFLLRGVGG
                     RRIAEPQLKGLRVQDRLAVLYCANDLHGALARDHLGNALFSCEPGGENQRYESWKLMV
                     NVILFSLTGTYKTDAIHQPFLERKAQP"
     gene            complement(12545..>13604)
                     /locus_tag="P5079_04645"
     CDS             complement(12545..>13604)
                     /locus_tag="P5079_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29310.1"
                     /translation="GSPDVAPVRSETYFLDPVLSSLAQKGLRFRNILQEELPKIDLDG
                     WDVVVLSNPIGLPAEAVSRLQEHLKRGKGLWITGGENVAGGALDYVSPGRFLPAVEVR
                     SPLQVPPEMGAHPIGQVLLPQGGVEWGEVHIGRACGFEPGIETSVLLSAGAGNPVLAV
                     GKVLGAPVAVLTTTLDRDWTNLPSKPLFPILCREVLAYLAGRSSVTGGSVLVGQPFEG
                     TLSDPLVSKVEIARPDGAVETVSVQNKHFAYAKTDLPGFYRLSRSAAGASLGGFVVNL
                     DRDADEGDLARLPMDKAEGLLPAENVFGVRFTENVHRAYMERLRGKDVFPQLAAAALL
                     LFLGETVLVAFAGRRRNA"
BASE COUNT         2945 a         4616 c         4161 g         1882 t
ORIGIN      
        1 cgccgaaatc gtccgtcgcg gtgaaagtca tcggcagttt ttcattggcc gacagcaaca
       61 aatcctgcgc cggggaaagg atctggacgt gcggcgcccg atcgtcctcc acctcgatcc
      121 gccagacaag ctcgtcccgg gccgccccgg acttcagcgc gaaatgcaac tcggcggatt
      181 cgtgcgcggt caacgacatt tcgagggcgt cgccgtcgtt cagcgtggcg ctccaaacgc
      241 ggccgtccgg agaagtgacc gccagatcgt ccactttcct atcgagccgc ccccgcgcgg
      301 tcaacgccgc cccccgcaac acccgcgtgg tcatgacgtt ctcctggcgt tcccgcggct
      361 ttcgggtata cggcggatag acaatatcga cccggaaccg ttcgaaacga ggcgggcgaa
      421 acggctcgat ccgataaacc ctgctccaac gcccctgatg acggaccttg taggacaccg
      481 gttccagcag gtttttcagc gtatgggaaa aatctctctc ggccgttttg aaaagatccc
      541 gcttctgcca ggtcgccccc ctcttcagcc acaattccgg ggaaaccgac ggcgtccgca
      601 gagtgacccg aatggggacg tcgccgccca cgggaacgcg ggcgtccccc ggatcgacgg
      661 agacgccggc gaggttttcg cggagcgcga acggacaata agccccccac aacgaggcgc
      721 gggcattttc cggcaaaagc aggaaaaggc ccgacacgag cagggaggtc gccgtcgcca
      781 gggcggtcca tcgacgccag ttccccggac gcatcgtttt cccgacggac aacggccgaa
      841 gccgctgaca gacccgctcc acctcccgct ccacgagttc cggcgacacg gcgcgtttcg
      901 cgatatcccc ccggcccatt tcccaggcgt tcagaacgca atccgccgga tggagggggc
      961 tccccaattc cgccgccacg gcggaggaac gccactgacg cgggcggacc ccattcgccg
     1021 cggccagggc cagggacccg gccgtcaaaa cgagccacgc ccccatccga acggccgggg
     1081 acaacggaaa cagccggtcc gcgaggaaaa tcgacatgcc cgacgcgccg aaaaaaacga
     1141 gggtcttctg aaaaatcagg aggacggcgt acccggcttt ccagaaacgc gcccgacgga
     1201 ggcgccgacg gatgaaagaa aggccgtcgg tcatgtctta tccgtccctc cgagcagatc
     1261 caccgcccag aggctctccc gccggaccat ttcgggtttc cccatccggc cgaaaagccc
     1321 cgcgagccgt tggtgcgaaa gcgcgtcccg cgtgacgtcg tagacgagca ggcttccggc
     1381 caggactttt tccggcgcgc ggtttttgag ccagtcgaaa atgtccttgt cggcgaaata
     1441 ggtgctttgc aggttcgtcg ccgagacgac caggagcgcc cggggctcgg cccgcaggtc
     1501 cacatcctgg tcggcaaagg cggggagggt gtacggggcc acggcggcat aggagatccc
     1561 ataggaggcc ggttccgccg tgccgaaata cgaaagatag atccggccga ccttctgttc
     1621 ggtcaagtat tgacgaagcg cgcgcatccc ctggccccag tccacgttgg aatcggtcag
     1681 atattcatac ccggcgacgc gggcggcggt gagggaattg aaataggaga ggaaccaagg
     1741 ccgggcggcg aacgtttcgg ccaaaagcca caccccgagg gcccacaggc cgtaacgggc
     1801 gggccgatgg gcccaaagcg ccgccgccgc gattcccagc atcacgaaca aaaagggata
     1861 cacgggcagg atgtgccgat ggccgatctg gaccgacgag gcgcaggcgg ccaggaaata
     1921 gaccaacggc ggcgtgacca cgagccaggg ccaccggacc ttcttgcgga gcgccagccc
     1981 cagggagaac aggaacccca ccaacaccgg ggtcgccgtc ttcaggaaaa agacggtcgg
     2041 gaaatagaac caccacccct cggtggagtg attccccaaa aggaaggtgg cgcggccgcc
     2101 ctgaagccgt tccagggtat accgcaaccc gccgaagaaa acgggcaggg cggtgacctg
     2161 atagaccaag agcaaggaaa ccgcggccgt ggcgaaacac accagcatcc cacggaaaga
     2221 aaggcccggc cggaatttcc ggtgaaacag ccacaacagc acggcggggc cgaccgccac
     2281 cgccgagaac ttgctgcaga acgccagcac cagcgacatc cccgtgaggc ccgcccagag
     2341 aaaggcccgc ggccccggac cgggcgcctc ctccgtcgag cgatcccacc ggtccagaaa
     2401 atagaaaaag aagaaataaa accaggtgaa aagataatcc gtggtcacca gactgccgtg
     2461 ggcgatgaat tccgggctga cggccgccaa aaggaacgcg accgaggccg ccacttcccc
     2521 gaaaacggac cggaccgcga agaaaaagcc gaccagacag acggcggaac aaaagagcat
     2581 ggcataccgc cccgcgttca ccatccggtc cgcgtccaag cccgaggcgt tgtggtagag
     2641 gaaacggttg gcgaaggcgt attggcgcgt cgggtcgccc caccgcggct ggagccagag
     2701 cgggtcgtcc tgcggcaacg ccggccccat gaaaaggagc ggaacggccc ccaccagcga
     2761 cgacaacgga ggatgatggt aggcgttgag tcgataatcg cgcgtcttcc aatagacgaa
     2821 acccgcggtg aggtggagcg gttcgtcgaa cgtgggggac tgggcccgaa cggccagccg
     2881 gcccgaacag acgtgcacgc cgatgcccaa caccagcaaa gggagccaga gaacggccgc
     2941 cctccgcgaa aaaacgggcg gcggcgaggc gggagttcgg cgcgcttcga tgttcttttt
     3001 cggattcgtc atcaaacctt cttctgtcgg ggatcgcacc gcccgtcggg gagaaagaga
     3061 cgctttcccc tcagagcgcg gcggcttctc gacccgatcc cttccgacga ccagggaccg
     3121 tttgcggcgg cgtccgcccg ctcgaagaat aacgcgaggt tattgttcga gcgaagtcga
     3181 gaacacaacg gctgccccac ggcaaagatg ccgcacccct cctgggcgcg gagtgtcact
     3241 ttttcaatgc ctgttcgcgc aacgcccgcg ctttgtcgag aaaataggcg gcctgggcgt
     3301 tccccgggtc ggcggccacc gcggcctgca tgtgggcgac ggcctggtcc cactctcggt
     3361 tccaataggt caccgcccaa ttgtagtggg cttgggagga tttcgggtcc aaccggaccg
     3421 cctgggcgta actgttcctc gccgcttcca aatcccccct cttctccgcc agcacgccgc
     3481 ggtggatcca cacctcggcc gcctcgcgac gcagcccctc caccacggcc gggaggcttt
     3541 tgtaataggc ggcgcggcgg aagagttcct caaaaatcct cgaggcccgc gcgtcggcgg
     3601 ccccgctttc ctccagccgg ccctgttgat aaagcacata gccccagttg aacggcaagg
     3661 ccggaaattc gctgtcgaag accatgccgc gccgcaggga cagcgccgcc tcgtcccagc
     3721 gcttctcgcg catcaacgcg gaggaggcgc ggagggccgc ccgggcgtat tccgtcagga
     3781 ggtccgaaga gaaaaagtcc ggggcccggg tctggcggcg ttccccgcga atcacataaa
     3841 actcgaacat ccggagaggg tcgaacagga ccgggccgac ctcggcgggc cacgtgagat
     3901 agatgaccaa cccggccggc ttcgtctcat ttttccagga aaggttggca tcgccgccca
     3961 cccacagcgg ctttcccgcc agagcgttca cgtaggactc ccacccgcgc tcgtcgtcca
     4021 agcggccgag cggaacgaac cggccggcct gtttcatggt ctcctgatac cacgggctgg
     4081 ccgccaaccc ctgggccacg atggtcacat cggggcgctg gtgcgacacc agctgccgtt
     4141 cccagaacgc gaaaagcggc acatcttccc ggagcacgcc gaaggagccg ggcaggctgg
     4201 accgcaacat attgcccacg taatcgcggg cgtacaggtt ccatcgttta tcgccccgcc
     4261 cccgatgcgc ggcggcgttg accgccacga gcgcggacac cgccgccgtc aaaaggagag
     4321 tcgttcgctt tccgccccct ctccaacgga acaccgccgc caggccggcc gccatcaaaa
     4381 gagccacgat gaggtgggag acggcgtaat gggcctccac gatggcgacg gcgtgcgggt
     4441 tgggcggcat gttggccaga aagaggaaca acggaccgaa caccgccagg ttgatcaaca
     4501 agaacgtcga aaaaaccggc aggcgtttcc ttaaatgtcc gccccccagg acggccagcg
     4561 ggagccccac ccaagtgaaa tccgacgcca gggttttcag atacaaaata aattccgaaa
     4621 cgaaattttc cccgagggcg taggatttcg acagcaggtc cagcgtgccg ccgtggctct
     4681 tccgcatgag agaacccaaa aagcggtcca atccctccgg gttgttccag tccacccacg
     4741 gctggcgggc ggaacgcagt ggaagataga gaaagacggt caggcccagg aaagcggcgc
     4801 cgagggccgg gaacagaatg cgccatccgc cgcggcgcca gcacaggtag gccagcgccg
     4861 gcgagagaag caaaaaatcc gtgcggacgc cgcaaccgac gccgaacaga aaagccgccg
     4921 ccggccacca acgggacgga cgcgggacgt gctcgtccga tatccgccgg acaaagacga
     4981 acaacaagac catgccgagc agaccgagcg tatacatctc ggagaccgag gaaagctccc
     5041 agaaaatggc ggaggtggcc cagagagcgg cggcccccgc cacggccgcc gaaccggtca
     5101 ccccccacat caagaggaac agccccgtct cggcggccgc cgaggaaagc gaggagagga
     5161 ggttgacgcg atacgcgggg ttcccccacc ccgattggac aaaaaccttg ccgagcagaa
     5221 cgtagagcgg atagcccggc ggatgggcca cgcccaaggt gtgcgcgtcg gtcaccatct
     5281 cgccggaatc gcgataaggc accaccgtgc cgccggcgaa atggagatgg aatccccaaa
     5341 aagccaggaa caggacgaac ggcagaacgc gccgcgtgcg cgcgccgaaa tcgtcttgtc
     5401 cagggagccg atccgcgccg ttcatgatcc tcccgaaaga cgccggcggg cctgcagaag
     5461 gaatttccgg gcctccgggt gggccgggtt ccaacgcaat acctgttcca gattctccat
     5521 gacggcgcgc cagtcgccga ggcgccagta gaccacgccg ctgttgtaat aggcttcggc
     5581 ataacgtggg tcccgctcaa tcgctttgcg gtaataccgg agcgcttctt ggaacttgtt
     5641 ctggtcggaa cataaaacgc ccaagttgtt ccaggccacg gcctcgctcc cgggccgccc
     5701 ggccagtcga cggtaaagcc gttcggcttc cgcccaatgt ccctgccggt gatgccatgt
     5761 ggcgaggtaa aagtcccagg ggctcattcc cgccttctcg cccatccgga tgaatcgttc
     5821 cgcctgccgg aggtcgttca acgcaaaggc gtaatcgccc atcaggcggg atgtcgaagc
     5881 cgccaagtgg agttcccgcg cgcccatggg gccgtccggc caacgccaaa gatagacgtc
     5941 ccaatacggg aacgcggtgc gggacgaaaa cgaggcggag gggcttgcct caaaaagcag
     6001 gccggcggcg cggcgaggca gcgcgagttg ggacacgtcc agcgtggcgt aataaaaagg
     6061 ccgggacgtg gacgccagca tccctccctc cacccgttgc cggcgggcca agcggggttt
     6121 tccccacacc tcgtaataat ccttgccgta caggctccga ctcacgccgg cgttggcgtc
     6181 cacaaaatcg acgtcgggcc ttttgaaggc cacatccatg agataggcca gggaaaattc
     6241 cgtctcatcc gcccggtcgg cgaacagata ggcgccggag ggggcggtcc gcaggacgtt
     6301 gcgtccgtgg tcgccgacgg ccaagtccca ccggtccgac ggcagagatc cgttcgcaaa
     6361 caccaccgcc gtccattgaa agaggcaaag ggccccgacg atctttcccc accgcccggt
     6421 ccgccacaag aacaagaacc cctccacccc gacgaagacc aacataaaga gcggcaaaaa
     6481 aaggaaccgt tccaacattt cacgcgtggc cgcctccggc cggatgttgg cccagaacag
     6541 gaacaacggc ccacaggcga gggcgcccag aaaccagacg gtctcctgcc gggcccagcc
     6601 gccctcccga atcccacgcc gcatccagac cgtcccgccc gtcagaagaa gcgccagccc
     6661 cccccaccgc caattttcca gcgtcttatc cccgaaaaac cggagttgcg tccaaagggc
     6721 attccaatcg tgcggcggcg gagttccctg agccaactgc aatgtcccat accgcgcccg
     6781 gctcaagact ttccagaatc ggtcccacgt ctcggggtct tcccaatcca gcaggggctg
     6841 ggaccgggac cgcaggtaaa ggaacacata caccgtgagc cccaaaaaaa agaaaacgcc
     6901 gacggccgcc aggaaccgaa aaaacgccgt cccacccttg agtccgccgg agacacgaag
     6961 ccgccccacc ccccaaagga acaggaacgg aaaaaccagg atcagcgtct gttgattccc
     7021 cagccagagg ccgaaaagga acaggctgag gaggaaccaa cgactcgaaa ccgtctcgca
     7081 aaacttcccc agcgccaacg ccaaaacgcc gacggccaga acgttcaagc cgaacacctc
     7141 ggcggcggtc gagagtttca agaacaacgg gctgagtccg gccagcagca ggacggcgtg
     7201 agcgggcgcg ggggcggaac gccacaggca cgcgaggagg gccagggcca gggccgtcga
     7261 aaacccggaa aacaggttca tccggaatgc cggattgccc cacggcacaa cgtgtccgaa
     7321 gacattcccc agaaggacat aaaacggata gccgggcgaa tgggcgacgc ccagggtggc
     7381 ggcggccgcg gtcaattccg ccgagtctcc gggcgccaga cacggttgcg cgccgcgcac
     7441 gtagaaaaac caaaggacga ggaaaagaac ggaaggggcc gcccatctcc atcccggagg
     7501 ggtctccatg aaaaaacgtt taacgtccgt catggcggcc ggcccgcctc tctcgagcgg
     7561 cggcgagata acgcccgccg ggatgttgcg gattgagggc gatgaccctc tccaacaaag
     7621 gcaccacccg ggcccagtcg cttcgccgcc agaagacgac cgccaggtta tagagggcgt
     7681 ccacgttgtc cggcgacaac cgcagggcgt tctcgtagct ccgcaccgcc tccgccggat
     7741 tcccccgcct ctcccacaac acgcccaggt tggaatagag ttccgatttc ggctgtttca
     7801 tccgcagggc cacgtcgtag aaaagtccgg ccgcctcgaa atccccggct tgcagacggt
     7861 catacccgaa ctcgagacat tgtcccatca gattctcctg cagccacggc acccgcccgc
     7921 ccatgcgacg ggtgcgttcc atgagggtcg cggcccgggc gtattgccgc tcctggaacg
     7981 aagcccgcga cgccatgaac ggaaaatagg gggccaacgc caaggtgcga tagtcctgac
     8041 tgggcggcgg atagagcgaa cggaaggcgt acaggtcgca cagccccgcg ttcccgtcgg
     8101 aggcgccggc ccgataaaga aatccggtca gcccgagagg taccccggga aggaggcggt
     8161 cgttcatggt ggaataatag agcggcctcc gggtcaacca gttccgttcc acgtcctgcc
     8221 ggcggcggtc tttcgcctcg cgaggcaggg aacggaaatc cgggccgtag gcgctggaaa
     8281 aaatgaggcc gccccggtcg aaaaccgcga ggtcgtccct ctgccggcgg acaaaccgtt
     8341 gatacgccat ggaatagaac gtgtcgtccc cgccgtccat gaagaaaacg gcgccgggcg
     8401 gcaaggtgcg caggatgttc tggctgtaat cctggaccaa gaaatctccc cgcaacggga
     8461 attcccggcc cacggaccac agctggaaca gcggaaacgc caggagcggc caggcccacc
     8521 gtttcagccg ggcctcggcg aatttccaga aaccgccggc ggcgagcacg ccgaacaccg
     8581 tcggcaggat gtaaaagcgg tccatgatgc cgtcggattg cgcgtcgaac gggaggttcc
     8641 ccaaccacat gaaaaaaggg cccgtgaaaa accaggcggc gaaaagtccg agggccgtcc
     8701 aacggaccga gcgccacagc aggaagaatc ccagaaccgc cgcgacggcc ccccacgggg
     8761 agagttgacg gacgctgact tttccgaacc gccgcatctg ttttgcggcc gtggccaacg
     8821 tcctgggcgg cgtttccccc agggccaggg aaaaacttcc gtaatctttt cgactcaccg
     8881 tgcggagtaa tccgttcaac gtctcggggt tcccccagtc caacggaggc tgcgcgcggg
     8941 accggagcgg caggaaagcg tacacgctga accccaaaag ggcgaacaac cccatcggca
     9001 ggagcaggcg cccgacgcgc cgaagccgct cctctcctcg ctccggcgaa ccgctcgacg
     9061 acgagaagca aaaaaggaac agggccgggg ccgtcaacaa cagggtatgg tggttcccca
     9121 accccagccc gaacaggaac gcccccacca cagcggcgcg agggcctccg gcgagaaaaa
     9181 gggccgcgac ggcgagcgcc agggcggtgt tgagcgcgaa cacctcggtc accaggccgt
     9241 tcaggaaaaa cgccggactg ccggccagca gcaggccgca gaacgcgccg ccccgccaag
     9301 acagcccccc ccgccgggtc gcgatgaaaa aaaccagccc gacgctgacg aggttcaacg
     9361 ccgccgacga gatgttgagg cggtagccgg cgttcgccca ggggaacagg gacgaaaaaa
     9421 gttttccgat cagggcgaac aacggatacc cgggggaatg cggcagcgaa agggtggcgg
     9481 cggcggtgat gaattccccc gaatcgccca ccgtcaccga cggggcggcg tggcgcaaaa
     9541 agcaggccca caggccggcc aacaacaccg ccccgacggt ccgctcccac cgcgaccttc
     9601 gcgtcagatc ttcccccacc gtcgcctccc cagccactcc ccgaaaagga gcaatatcaa
     9661 caccgcaaaa atccccttgg cgttccagac ggcctcgcgc cgctcgaagc tccacgccga
     9721 cgatgaaacg ttcttttcca gccattcgct tgagaagccg gcggccggaa cgaattcgcc
     9781 gccgccggcg gacgcccacc gcgtcaaaga cgggaaatcc gtggaggtgt cccggttctc
     9841 cacccaaagg cgcgcgcatc ggacgacgcc gctgtcttcc cccagtttcc tcccgttgct
     9901 gaaagcccgt acgctgacgc gataaagccc gtccttctcc ggcacaaaag cgtcggcgta
     9961 ttcccccttc cccaaaaagg cgaggtccct ctgccgggcg gcgccggcgg ggtccgtgat
    10021 gacggcccgc acctgggccg aattcatcgg gcgataattc tcatcgcgca cgaacgcctt
    10081 gaggaagatt tcctctccca aagacaccgg ctcgttgggc acgctgaggc gcaccaaacg
    10141 gaattcgcgg gacgccgaca gccacctcag aagattgttc cagaatttct gataacccgc
    10201 ggccccctgt ccccgccccg cctcttcgag cgcccaacgc caggacgtca gggaaaggaa
    10261 catggccgcg cggccacggc cccgggtcca ggccgccaac acgggcagcc ggccgtcggc
    10321 ggcggggcat ccggccacca ccacggcgcc cggccgggtt tttgcaagga actgcccgtc
    10381 cccgtccaac gcgggcatcc gcgcccaaag cgcgcgcgcg gcttcggggt tctcttcgat
    10441 ggccatcaac ggatgatccg gcgcatagga ttccaaaatg taccgctgcg gtcccgcgcg
    10501 aagcggagaa tccaacgcga acggcaggac ctccgccacc ggcgtgccga cgtagggccc
    10561 cgcggggccc agcatccgcg cgttcccgat caacagcagc cccccgccct tttccacata
    10621 gtcccgcagg gccgccatgg ccgaaggccc caggccgaac gaggaaaacg cgaactgttc
    10681 caggacaatg atatcaaaag aattcatctg gccgacgagg acgtcctggg tcggaaaagg
    10741 gatgagggcc agttggttgt ccggaacgcc cacggcgtcc tccggatcgc gcaggatgac
    10801 gaaggagacg agctccacgg acggattggt cttgagttga tgccgcagga acgcgtaatg
    10861 cggacccggc tggccggaga tgtacagaac ccggacgcgg tccctggaaa cgtccagggt
    10921 gaacgcctgg ctgttgttct tccgattgga ttccttctca tagaccggca ggcgcacgga
    10981 atacgtctgc aggcccacgg aatccggccg gaagacgagc acggtttccg cggcgtcgcc
    11041 gccggcgaac tccacggtct tggccgccac gctctcgtcg ccccgaagga cctggaccgt
    11101 caaccggtcc accgtgatgt tccttttttc gacgcggacc accacctcca cgtccgtgtt
    11161 cttaaaagcg aaatgcggcg cgcgaaggga cgccaacagc acatcgggcg cggtctccgg
    11221 ttttcccacc ccgatcgcat agacggggat ccccaaatcg gaaaaagcgg ccgccggatc
    11281 ccgtcccccg ccggcgcggc cgtcggaaaa aaagaagacc gcccgcaccg acggcgcctt
    11341 ccgcgggatc tccccgatcc gggcgaaatc ggaggccggc ccgtcgggcc gcacggcgga
    11401 cagcacgccc cccggcgcct ctttcaggcg ggaccccagg acataataga ccggacggca
    11461 caaccgttcc cagtcgggcc gggtgagttc cagtttcgac aaggcctcgt tccaacgcga
    11521 aacatccccg gcctctccgg cgtcccgcac cgccatgctt tcggaatggt ccaccacgat
    11581 ggccacgggc acgcgttcca cccggacgcg ccgttcgacg accggctgaa agaaaatggc
    11641 cgtcaacaag atcccttcca ccgcgcgcac cccgatgagg aggagcgaac ggcggcccga
    11701 cacccgaccg cgccacgtca aaagggcgaa caccgacagg gccgccagga gcgccagcca
    11761 caccagatgt tcgacggcgg tcaaggcgcg cagatggacc aacaggtcgt tcatggctgc
    11821 gccttgcgtt ccaggaaagg ctgatggatc gcgtcggttt tatacgtccc ggtcaaactg
    11881 aacaggatga cgttcaccat gagtttccac gactcgtacc tttggttttc cccgccgggc
    11941 tcgcaggaga acagggcgtt ccccagatgg tcgcgggcca acgcgccgtg gaggtcgttg
    12001 gcgcaataaa gcaccgccag ccggtcttga acgcgcagtc ctttcaactg gggctcggcg
    12061 atgcgccgac cgccgacgcc gcggaggagg aaaaaggacc ggaacaccac gttgtcggac
    12121 gggagcgcct cccaggtcga ctccggaaaa agcgatttca ggagggccgg cgcgcttcgg
    12181 ccgaacggac tgttccgttc ggcctcggtg ttgtccagaa atatcatccc accgccgatg
    12241 atgtaccgac gcagcctttc cttttccgcc tcactgaagg aaacggcgcc ttgtcccagg
    12301 atgaccagga acggcgactc gaacagggcc gggtcgtcta ccgtcagggt ccgtctttcg
    12361 ggccagggaa ccacgctggt ggtctgctgg agataccgcc cgatctgggg ccacagggcg
    12421 gggtaggggt cccacgtccc ctgccatcgc agctgcgcat agacgaaacg gttctccggc
    12481 cccccccctt ccgccgaaaa aaccgtggag gcgaaaaaca tcaaccccgc cagcatcaaa
    12541 cgcctcacgc gttcctcctc cggccggcga aagcgaccag aacggtttct cccaaaaaca
    12601 acagcagggc cgccgccgcc aattgaggaa aaacgtcttt tccccgaaga cgttccatgt
    12661 aggcgcgatg aacgttttcc gtaaagcgca ccccaaaaac gttctcggcc gggagcagtc
    12721 cctccgcctt gtccatggga agccgggcca aatccccctc atcggcgtcc cggtcgagat
    12781 tgaccacaaa accgcccaga gacgcccccg cggcggaccg ggacaaccga tagaaccccg
    12841 gaaggtcggt cttcgcatag gcgaaatgtt tgttttggac agaaacggtc tcgacggcgc
    12901 cgtccggccg ggcgatctcc actttcgaca ccaacggatc ggacaaagtc ccctcgaacg
    12961 gctgccccac caagaccgaa ccgccggtca ccgagctgcg tcccgccaga taggcgagca
    13021 cctcccggca gaggatcggg aaaagcggct tcgacggcag gttggtccag tcccggtcaa
    13081 gggtggtggt caaaaccgcc accggggcgc ccaaaacttt tcccacagcc aacaccggat
    13141 tcccggcgcc ggccgaaagg aggacggagg tttctattcc cggctcgaaa ccgcaggcgc
    13201 ggccgatatg gacctctccc cactcgaccc ctccctgcgg cagaagcacc tgcccgatgg
    13261 gatgcgctcc catttccggc gggacctgca ggggcgatct cacctcaacc gccggcagga
    13321 atcggccggg ggagacgtag tccagcgcgc cgcccgccac gttctccccg ccggtgatcc
    13381 acaacccttt tccccgtttc aagtgttcct gaagacgcga caccgcctcc gccggcaagc
    13441 cgatcgggtt cgacaggacc accacgtccc agccgtcgag atcgattttc ggcagctcct
    13501 cctggaggat gtttctgaaa cgaagcccct tttgcgccag ggacgacaag accggatcca
    13561 aaaaatacgt ctcgctgcga acgggggcca cgtccggcga accg
//
LOCUS       DAOTFD010000050        13495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3172_length_13495_cov_5.539062, whole genome shotgun sequence.
ACCESSION   DAOTFD010000050 DAOTFD010000000
VERSION     DAOTFD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13495
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3172_length_13495_cov_5.539062"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2559)
                     /locus_tag="P5079_04650"
     CDS             complement(<1..2559)
                     /locus_tag="P5079_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRY29311.1"
                     /translation="MPPLGIPKKQGLYDPRFEHDACGVGFVVDIKGRKSHAIVRQGLT
                     VLNNLVHRGACGCEPNTGDGAGILFQIPHAFFSRVGAREGLPLPAAGGYGVGMIYLPP
                     NPAHRQACEKRFESIVAEERQTVLGWRTVPTDNSPLGPSAKSGEPVIRQIFIGRNPSL
                     TDDMSFERKLYVIRRRAENALRRGTPEGAGFYVASLSSRTIVYKGMLLPDQFDCYYPD
                     LLDPDVASALALVHSRFSTNTFPNWERAHPYRYVIHNGEFNTLRGNVNWMKAREAQLS
                     SNLFGDDLPKILPVIDEEGSDSAMFDNCFEFLVLAGRSLPHAAMMMIPEPWENHESIS
                     PEKKAFYEYHSCLMEPWDGPASICFTDGTRVGAVLDRNGLRPSRYYVTKDDLVVLASE
                     VGVLDIPPERILHKGRLKPGRMLLIDTEKGRIVSDKEIKQTIAAEHPYGDWLKQHRLF
                     LSDLPTPEGTSAPLEGKKLHQQQQAFGYSYEDVRILIEPMSLTGVEPIGSMGTDTPLA
                     VLSERPQLLYNYFKQLFAQVTNPPIDAIREEIITSSRMPLGPERNLLNPEPDSCRQIH
                     LQTPVLTNEELERIRGARRPGFQAATVPMLFSPSGNGDGGRALEKALEDFFKDADRAV
                     AGGANILILSDRGVDAAHAPIPALLAVAGLHHHLIRQGLRTRVGLVVESGEPREVHHV
                     ALLIGYGASAVNPYLAYDTIRDLVLQNMLPGTDFETAQRKYVKSLVKGTVKTISKMGL
                     STVQSYRGAQIFEAVGLTRSVIEKYFTGTPSRVEGVGLDVLAKEAALRFERAFNERAV
                     DVAVLDPGGQYQWRKDGEQHLFNPVTIQLLQRACRTNDYRTFKEYTEQINNRTKT"
     gene            complement(2587..3531)
                     /locus_tag="P5079_04655"
     CDS             complement(2587..3531)
                     /locus_tag="P5079_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY29312.1"
                     /translation="MQIEVLKLFCDVVRFHSFSRSAEENNITQSNASQAVQNLEERLG
                     VKLIDRSLRPWKLTNEGKTFYEGCRTLVDRYYELEREVKNSHDETTSIVRVASIYSVG
                     LRHMRYYVQKFSELHPKAHVALEYLHPERIYESVAQDQIDLGILSFPQPRRDIAVIPW
                     KFEPMALACAPEHRLAHSREVDPSHLAGEKFVAFDRGLGIRSEIDRYLKQHGVEVQVT
                     LEFDNIEFIKQAVEIGSGIALLPRPSLDREVAAGTLTAVPLSTKNLVRPLGIIHRRGK
                     IFTPNVTRFVELLQNEKEPELSSAAAAPVRSKKPTIDR"
     gene            complement(3656..4855)
                     /locus_tag="P5079_04660"
     CDS             complement(3656..4855)
                     /locus_tag="P5079_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29313.1"
                     /translation="MTNALFLLERAERAQAAGRNALAERLLQRAGRRAPDDFLRAEII
                     WRRAEALRALGRFREALSFYWRAHALYRRCGVSSERLRALLGASACLRILGRFSQAGR
                     LWRTVLRQKYVRRVPDPAPAECLLELALSERGAGRLLSARRLLLACLRQAKRGKDTEL
                     LRHIHWALGGTERFLGHFPKALASFKRAEDLARKTGDAAARAYALCGQAGTLRLLGRG
                     ADSYRRYRQAHGVFKRLRDPFGTAYGLCGMGNALRTYGNARRSIPLYVRSAEIYARVG
                     DESSRAFALWGQSGAERRLGHPRSAARLGAQSLELFLQEKDRRGEIMASLGLARLLKE
                     QGRTAEALLYIGKAAQTAHRAGLHYESALARFEKMRLRFPRRDFSSLFRSFGIPAPAV
                     RRWQDLP"
     gene            complement(4929..5348)
                     /gene="rpsI"
                     /locus_tag="P5079_04665"
     CDS             complement(4929..5348)
                     /gene="rpsI"
                     /locus_tag="P5079_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY29314.1"
                     /translation="MTNETLSITRQSPLWATGRRKTAVARLRIVPGEGRFIVNAKSLD
                     EFFAGHERQKSSAMAPLKLTKPMTNMDIFVSTTGGGVTGQAEAIRLGLSRALVAMDPK
                     LRSALRAKGFLTRDPRMVERKKSGQPKARKRYQHSKR"
     gene            complement(5353..5877)
                     /gene="rplM"
                     /locus_tag="P5079_04670"
     CDS             complement(5353..5877)
                     /gene="rplM"
                     /locus_tag="P5079_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY29315.1"
                     /translation="MLQTQTPRLKTKDISRRYFLLDGNGVVLGRLATLAANLLRGKGN
                     PAYSPAMDIGNHVIVINADKVALTGNKLIQKIDFRASGYPGGQRFQNYGRLMKEKPER
                     AVKLAVYGMLPKNRLRGRFMKRLKIFRGAEAQKKFAGAETVDFSNPKMRSGGPYVSRP
                     ALKTAPAPTGGEAS"
     gene            complement(5911..6513)
                     /locus_tag="P5079_04675"
     CDS             complement(5911..6513)
                     /locus_tag="P5079_04675"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRY29316.1"
                     /translation="MIKIRRAKINDVRTIHKLLNGFADRKELLPRAISELFENLQQFH
                     VADHGGAVVGCCSLAVQWDNLAEIKALAVADAYQGKGIGKKLLQTCLADAEALGITRI
                     FTLTMKDGFFNKFGFKRVVKNNLPHKVWTECVRCPYFPETCVEIAMVKDFDARQKPIP
                     AFKPGDLPKDGTVPTEPMVPKEPEADAPKTATRRRRAARP"
     gene            complement(6563..10168)
                     /locus_tag="P5079_04680"
     CDS             complement(6563..10168)
                     /locus_tag="P5079_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY29317.1"
                     /translation="MDKRFLKEDGMEAFTGNELLLKGALEGGMALMTGYPGSPISEVF
                     DAASSVADLFTERGIVAQIANNEALSAARLNGARLANLRAMSLMKSVGLHVAADGLAI
                     GNLAEPQNHDGGAVVVVGDDPWNETTQINSDSRFLSQHLNMPVLEPATFQELKDWIKD
                     GFELSGQANLYITYVITTNQADGGGAVRCRPNLYPVVNNNQKTALSSANMAVENTVMI
                     PPHTSWREATLAARFDRLKTLVRQKGLNRIDWGAAGEKSSAGFAASGLSYCYLMQALR
                     EMGLENRFPVLKWGVTFPLEEDILMEFASRVDQIYVIEEKRAFVETQMVHLLQRAVQT
                     GRLKRMPAVWGKKFPDDQDGIPSIRGINTSILMERLGGLFKQRLLPLPSDAGARWEKA
                     HRTVTDTAAFDVKIPARTPTFCPGCPHRDSSTVAKSLKRDFLDAAYMRKKYDRDPLDV
                     IFHGESGCHSMLQFEPNLGLMQDYSGMGLGGGTGAGLSPFVKNKHVVFVGDSTFFHSG
                     MLAVSDAIKNNQDITFVILDNKTTAMTGHQPTPGNAINLLYQKTFAQDIEQVIRGMAR
                     DSIPVYRVNPAYRNSYRTLMEGVILKDGVKVIIADKECGITYHRRVRRERKKIIRLQG
                     YLPEEKVINATPEVCEFCLECTNDTGCPGLAIENTLHGPKVVTDRSLCIADAACTKAK
                     VCPSFEEITIRRRQPPAPLPEAVVGSLPLPPLKEVSDTWFCYTAGVGGTGAGVVTGVL
                     VRAGLRQGYHAIFADKKGLAIRNGGVYGHVIFSREEQVLSPLVPYGHADLLLGIDLLE
                     ATRGLDPKVNLRVASPTRTVAVMNTHKIPTIRTLLGKDDFSPQDLEDTVKRYTRADDY
                     LGLDFSNVSETYFGNKLYANVLLIGAAFQRGLLPVSLDNLNEAIRATVPPHELEENLR
                     ALQLGRQIGLRPELFKKFTVRLPSYRETLDDKTGLLERSRFLIGKRLAREYRKMVEDA
                     VRWMELPEPDRLKLAQYVYDLIQHSGLPYARRYLARLWSTYKKDQAEQNYAATRAVLD
                     NLYKAMLIKDEVYVAHLLTSEEKFRRDRERYRADESRGDRFEYVHLTRPQFTLMGRDF
                     EFDIRTRNWQLRLMKHLRPLRTLLPEWHAREKSFRRWYEDLVDGFQLFSDPETYRLYT
                     QALRLPEDVRGYRAVRYPKMEAARKKAEELLEALHRRRAASGRTALRKPNDRRAE"
     gene            complement(10192..11880)
                     /gene="recN"
                     /locus_tag="P5079_04685"
     CDS             complement(10192..11880)
                     /gene="recN"
                     /locus_tag="P5079_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRY29318.1"
                     /translation="MLNYLSIKNFALIEDMFLEFTPGLNVLTGETGAGKTIVIEALGL
                     VLGDKASASQARKGAARLSVVAEFRLSAPRLKRLLRELDLAAEEGDQLLLRRELDAGG
                     RSRCFVNDNPVNLSTLARIGEHLAYIHGQHEHQLLLKSSEQLDVLDTFGRLEPLRQTT
                     AEAHGRWKALLAEQQAVSLSDQERAQRVDLYRFQLTELKTAQLKTEGEDEDLERLLPQ
                     LKNAERLKNLSQQSYDLLYGSDGSALDNVRRVQRDVETLQSLGGDLRTIPDMIAESAV
                     RLEEAAHELEAFKNRVDLDPERLDEVLSRLDVLGKLKRKYGPALKDVMAHQTKIEEEL
                     KYLENAEERTRDLSEKLAKAQSELKDVCLKLSQARTAAAKKLAAAVEKEFHEVGLPHA
                     AFDVAVEKNPDGPATAAGFDAVRFLFSPNPGEGRQPLADIASGGELSRVMLALKSVLA
                     RADEVPTLVFDEIDAGVGGRLGGVIGKKLARLGKTHQVLCITHLAPIAACAQNHFYVE
                     KHVVKDRTSTTIKKLQEEGRVLEIARMFGGAAEKTKDESISVKHAKELLATSRT"
     gene            complement(11917..12729)
                     /locus_tag="P5079_04690"
     CDS             complement(11917..12729)
                     /locus_tag="P5079_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRY29319.1"
                     /translation="MTPTPKCVVVFHNAAKKLARRELSRLSAWFRRHGIRTVPPARAR
                     EAQVAVALGGDGTLLSAARRLAPWGIPALGVNLGHLGFLAATDLHRMYQTLEALLKGR
                     LVCSSRMMISVTAPRQKPRLALNDCVIRVSSTVRTIRLSARVNGEYLGTFVGDGLILA
                     TPTGSTAYSLAASGPIAQPEMDLLLLTPICSHSLTQRPVILSADSVLDISVEARAKRD
                     KVLLSLDGQTDFPLRTGDRIRVERATERFQLFLNPQRPYFSLLQQKLRWGER"
     gene            complement(12758..>13495)
                     /locus_tag="P5079_04695"
     CDS             complement(12758..>13495)
                     /locus_tag="P5079_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY29320.1"
                     /translation="TRFIPIIMMTGLSPEQEKLRALEAGCDDFLTKPIQHAELLARIK
                     SLLRMKSLTDELEDAEVVLFTLARTIEAKDQYTLGHADRVGRFALALGKALSLDTRKL
                     ELLRKGGILHDIGKLGVPDAILQKEGPLDKDEWETMRKHPTIGCDICSRLKVMQEIIP
                     IIRHHHERLDGTGYPDGIKGSKIPDVVRIVSMVDIYDALTSRRSYKEAFAPAKALRIL
                     HEEVDRGWWDGNILKIWEELVRKNGNS"
BASE COUNT         2190 a         3722 c         4830 g         2753 t
ORIGIN      
        1 ggtttttgtc ctgttgttga tctgttcggt gtattccttg aacgtgcggt agtcgttggt
       61 gcggcaggcg cgttggagca gttggatggt gacggggttg aagagatgtt gttccccgtc
      121 ttttctccac tggtactgtc cgccgggatc cagaacggcc acgtccacgg cgcgttcgtt
      181 gaaggcccgc tcaaagcgca gcgccgcttc tttggcgagg acgtccagcc ccacgccctc
      241 cacgcgggaa ggggtgcctg taaaatattt ttcgatcacg gaacgggtga gccccaccgc
      301 ctcgaagatc tgggcgccgc ggtagctttg cacggtggag agtcccattt tggagatggt
      361 cttgaccgtg cccttcacca gggatttcac atatttcctt tgcgccgttt caaagtccgt
      421 tcccggaagc atgttttgga ggacgaggtc gcggatggtg tcgtaggcga gataggggtt
      481 caccgcggag gcgccgtatc cgatgagaag ggccacgtgg tgcacttcgc ggggttcgcc
      541 cgattccacc accagcccca cccgggtgcg gagtccctga cggatgaggt ggtgatgcag
      601 tcccgccacg gccaaaagcg ccgggatggg cgcgtgggcg gcgtccactc ccctgtcgga
      661 caggatgagg atgttcgccc cgccggccac ggcccggtcg gcgtccttga agaaatcttc
      721 cagcgctttt tccagggccc ggccgccgtc gccgttgccg gagggggaaa aaagcatcgg
      781 cacggtggcc gcctgaaacc cgggccgccg cgcgccccgg atgcgttcca gttcttcgtt
      841 ggtgaggacg ggcgtttgaa gatggatctg gcggcagctg tccggttccg ggttcaggag
      901 gtttctttcc ggtcccagcg gcatgcgtga ggaggtgatg atttcctcgc ggatggcgtc
      961 gatgggcggg ttggtgacct gggcgaagag ctgtttgaaa tagttgtata agagctgcgg
     1021 ccgttcggag aggacggcca gcggggtgtc ggtccccatg gaaccgatgg gttccacccc
     1081 ggtcagggac atgggttcga tgagaatgcg gacatcctcg taggaatagc cgaaggcctg
     1141 ttgctgttga tggagttttt tcccttccag cggggccgag gtcccttcgg gggtcggcag
     1201 gtcggagagg aagaggcggt gttgtttcag ccagtcgccg tagggatgct cggcggcgat
     1261 ggtctgcttg atctccttgt cggacacgat gcgtcccttt tccgtgtcga tgaggagcat
     1321 gcggccgggt ttcaggcggc ccttgtggag gatgcgttcc ggcgggatgt cgagcacgcc
     1381 cacttcggag gccagcacca ccaggtcgtc cttggtcacg taatagcggg aggggcggag
     1441 gccgttccgg tcgagcaccg cgcccacgcg ggtgccgtcg gtgaagcaga tggaggcggg
     1501 gccgtcccac ggttccatga ggcagctgtg gtattcgtag aaggccttct tctcggggct
     1561 gatggattcg tggttttccc acggttccgg gatcatcatc atggcggcgt ggggcaggga
     1621 gcggccggcc agcaccagga actcgaagca gttgtcgaac atggccgagt cgctgccctc
     1681 ttcgtcgatg accggcagga tcttcggcag gtcgtccccg aacagattgg aagacaattg
     1741 ggcttcccgc gctttcatcc agttgacgtt gccgcgcaag gtgttgaact cgccgttgtg
     1801 gatgacgtag cgataggggt gtgcgcgttc ccagttgggg aaggtgttgg tgctgaaacg
     1861 cgaatgcacc agcgccaggg cggaggcgac atcggggtcc aggaggtcgg ggtaatagca
     1921 atcgaactgg tccggcagga gcatcccctt gtagacgatg gtccgggagg aaaggctggc
     1981 gacatagaac ccggcgcctt cgggcgttcc ccggcggagg gcgttctccg cgcggcgccg
     2041 gatgacataa agtttccgtt caaaggacat gtcgtccgtc agggacggat tccgtccgat
     2101 gaaaatctgt cggatcaccg gttcgccgga cttggcggag gggccgaggg gggagttgtc
     2161 cgtgggcacg gtgcgccagc ccaggacggt ctggcgttcc tcggccacga tggattcaaa
     2221 acgtttttcg caggcctgcc ggtgggcggg gtttgggggg aggtagatca ttccgacgcc
     2281 gtaaccgccg gcggcgggga gagggagtcc ctcccgggcg ccgacccggg agaagaaggc
     2341 gtgcggtatc tggaaaagga tgcccgcgcc gtcgccggtg ttgggttcgc agccgcaggc
     2401 gccccggtga acgaggttgt ttaggaccgt gaggccctgc cgcacgatgg cgtgcgactt
     2461 gcggcccttg atgtccacga cgaacccgac gccgcaggcg tcgtgttcga agcgcgggtc
     2521 gtaaagtcct tgttttttgg ggatgccgag cgggggcatg ggtttctcct gtggggcgtc
     2581 gaaaaatcaa cggtcgatgg tcggtttttt ggaacggacg ggcgcggcgg ccgcgctgga
     2641 aagttccggt tccttttcgt tctgcagcag ctccacaaaa cgggtgacgt tgggcgtgaa
     2701 gatttttccg cggcgatgga tgatccccag cggacgaacc agattcttgg tggagagcgg
     2761 caccgcggtc agcgttccgg cggccacctc gcggtccagc gaggggcgcg ggaggagggc
     2821 gatgcccgac ccgatctcca cggcctgttt gatgaactcg atgttgtcga actccagcgt
     2881 cacctgcacc tcgacgccgt gctgtttcag ataacggtct atttcggagc ggatccccag
     2941 tccgcggtcg aaggcgacaa atttttcgcc ggcgagatgg gacggatcca cttccctgga
     3001 atgcgccagg cggtgctcgg gggcgcaggc cagggccatg ggctcgaatt tccacgggat
     3061 caccgcgatg tcccggcggg gctggggaaa agagaggatg cccaggtcga tctggtcctg
     3121 tgccacgctt tcatagatcc gttccgggtg caggtattcg agggccacgt gggctttggg
     3181 gtgaagttcg gaaaatttct gaacgtagta gcgcatgtgg cgcaggccca ccgagtagat
     3241 ggacgccacc cgcacgatgg acgtggtttc gtcgtgggag tttttgacct cccgctccag
     3301 ttcgtaatac cggtccacca gggtgcggca tccctcgtaa aaggtttttc cctcgttcgt
     3361 caatttccac ggccgaaggg agcggtcgat gagtttgacg ccgagccgtt cctccagatt
     3421 ttggacggcc tggctggcgt tggattgggt gatgttgttt tcttcggccg agcgcgaaaa
     3481 actgtggaac cgcaccacgt cgcaaaaaag tttgaggacc tcaatctgca cgggaacctc
     3541 ccaatatcat tattacttat ccaacataga cactatataa attaatctga tatatgtcaa
     3601 taaaaaaatc cgccgcggac ggacggtttt ggagaagtaa gaggaaggga acgaattacg
     3661 gcaggtcttg ccagcgacgg acggccgggg cgggaatacc gaacgaccgg aacaaggagg
     3721 aaaaatcccg gcggggaaag cggagccgca ttttttcaaa acgggccagg gccgattcgt
     3781 agtgcaggcc ggctcggtgg gccgtctggg cggccttacc tatataaagg agagcctccg
     3841 ccgttcggcc ttgttctttg aggagccggg ccagacccag agaagccatg atttcgccgc
     3901 gccggtcttt ttcctggagg aacagttcca gggattgtgc gccgagtcgc gcggcggacc
     3961 gcgggtggcc gagccgccgt tcggcgccgg attggcccca caaggcaaag gcgcggctgc
     4021 tttcgtcccc cacccgcgca tagatttcgg cggagcggac atagagaggg atggagcgcc
     4081 gcgcgtttcc atacgtgcgc agggcgttgc ccatgccgca gagaccgtag gcggtgccga
     4141 aggggtccct caggcgtttg aaaacgccgt gagcctgtcg gtatcgccga taagaatcgg
     4201 cgccccggcc gaggagtcga agcgttccgg cctgtccgca gagggcgtag gcgcgggcgg
     4261 cggcatcgcc ggtttttcgg gcgagatcct cggctctttt gaacgaggcg agcgcttttg
     4321 gaaaatggcc gaggaaacgt tccgtcccgc ccagggccca gtggatatgg cggaggagtt
     4381 ccgtgtcctt cccgcgtttg gcctgccgga ggcaggcgag gaggaggcgt cgcgccgaaa
     4441 ggaggcgacc ggccccgcgt tccgacaggg ccagttccaa aagacattcg gccggggccg
     4501 ggtcggggac gcgtcggaca tatttctgtc ggaggaccgt gcgccagagg cggccggctt
     4561 gagaaaaacg gccgaggatc cggaggcagg cgctggcccc gagaagcgcc cggagccgtt
     4621 cggaagacac gccgcatcgg cggtagagcg cgtgggcccg ccaatagaag gagagggctt
     4681 cccggaaccg gcccagggcg cgaagggctt cggcccgtcg ccagatgatc tccgcccgta
     4741 aaaagtcgtc gggggcccgt ctcccggcgc gttgcaggag tcgttcggcc aaggcgtttc
     4801 ggccggccgc ttgggcgcgt tccgcccgtt caagaagaaa gagagcgttg gtcatgggag
     4861 ggagatcggg aaaatacgtc ccgcccgccg aggggcggag caggggacga cgcgtcggaa
     4921 gagcgaagtt atcgtttgga gtgttggtaa cgctttcggg ctttgggttg gcccgatttt
     4981 ttgcgttcca ccatgcgcgg gtcgcgggtg aggaaccctt tggcgcggag ggcggagcgg
     5041 agcttggggt ccatggccac cagggcgcgg gaaaggccca gccggatggc ttcggcctgg
     5101 ccggtgacgc cgccgccggt ggtgctgacg aagatgtcca tgttggtcat gggttttgtg
     5161 agtttcaaag gggccatggc cgaggatttt tggcgttcgt ggccggcaaa gaattcgtcc
     5221 agggatttgg cgttgacgat gaagcggcct tcgccgggca cgatgcgcag gcgagccacg
     5281 gcggtttttc gccggccggt ggcccagagc ggggactgtc gggtgatgga aagcgtttcg
     5341 ttcgtcatgg ggtcaggagg cctctccgcc cgttggggcg ggcgctgttt tgagcgccgg
     5401 ccgggaaacg taaggtccgc ccgaacgcat tttggggttg gaaaaatcca cggtttccgc
     5461 gccggcgaat tttttctgcg cctcggcgcc gcggaagatc ttcagccgtt tcatgaagcg
     5521 gccgcgcagg cggtttttcg gcagcatgcc gtacacggcc agttttacgg cacgctccgg
     5581 tttttccttc atcagccgcc cgtagttctg aaatctttgg ccgcccggat agccggaggc
     5641 gcggaaatct attttctgga tgagtttgtt ccccgtgagc gccaccttgt cggcattgat
     5701 gacgatcacg tggttgccga tgtccatggc gggggaatag gccgggttcc ccttgccgcg
     5761 cagaaggttc gccgccaggg tggccaaccg gcccaggacc acgccgttgc cgtccaggag
     5821 gaagtatcgg cgcgagatgt ctttcgtctt taatcggggt gtttgcgtct gtaacatagg
     5881 cggagagtct agcaaagcgg gccgtgaccg tcaagggcgc gcggcgcggc gtcggcgcgt
     5941 ggcggttttg ggggcgtccg cctcgggttc tttcgggacc atgggttcgg tgggcacggt
     6001 gccgtccttg ggcaggtctc ccggtttaaa ggccgggatc ggtttttgcc gggcatcgaa
     6061 gtccttgacc atggcgattt ccacgcaggt ctccgggaaa taggggcacc tcacgcattc
     6121 ggtccagacc ttgtggggca ggttgttctt gaccacgcgc ttgaatccga atttgttgaa
     6181 aaaaccgtct ttcatggtga gggtgaagat gcgggtgatt ccgagcgctt cggcgtccgc
     6241 aaggcaggtc tggaggagtt ttttcccgat gcctttcccc tggtaggcgt cggcgacggc
     6301 cagcgccttg atttcggcga ggttgtccca ctggacggcc aatgaacagc aacccaccac
     6361 ggcgccgccg tggtcggcca cgtggaactg ttgcaggttc tcgaaaagtt ccgagatggc
     6421 ccgcggcagg agttccttgc ggtcggcgaa accgttcagc agtttgtgga tggtccggac
     6481 gtcgtttatt ttggcgcgtc ggatcttgat catggaggtc ccttctcttt tttaggaaga
     6541 aaaaatacgg cggtcgacgg gactattcgg cccgccggtc gttcggtttt cggagggccg
     6601 tgcgccccga ggcggccctc cgccggtgaa gggcctccag cagctcttcg gcttttttgc
     6661 gcgcggcctc cattttggga tagcgcacgg cacggtagcc tcggacgtct tcgggcaggc
     6721 gcagcgcctg cgtgtagagg cggtaggtct cagggtcgga gaaaagctgg aagccgtcca
     6781 cgaggtcctc ataccatcgg cggaaggatt tctcccgcgc gtgccattcc ggaaggaggg
     6841 tccggagcgg gcggaggtgt ttcatcaggc gcagctgcca gttgcgcgtg cggatgtcga
     6901 actcgaagtc ccttcccatc agggtgaact gcggccgggt gaggtggacg tattcgaaac
     6961 ggtccccccg cgactcgtcc gcgcggtatc gctcccggtc gcgccggaat ttctcctcgc
     7021 tggtgaggag gtgggccaca tacacctcgt ccttgatgag catggccttg tagaggttgt
     7081 cgagcacggc gcgcgtggcg gcgtagttct gttcggcctg gtcttttttg taggtgctcc
     7141 aaaggcgggc gaggtaccgc cgggcgtaag gaaggccgct gtgttggatg aggtcgtaaa
     7201 cgtattgcgc cagtttcaga cggtcgggtt cggggagttc catccaccgc acggcgtcct
     7261 ccaccatttt ccggtactcg cgggcgaggc gtttgccgat caggaaccgg cttcgttcca
     7321 gcaggccggt tttgtcgtcg agcgtttccc gatacgacgg caggcggacg gtgaatttct
     7381 tgaaaagttc gggacggagc ccgatctgtc gtccgagctg cagcgcgcgg aggttttcct
     7441 ccagttcgtg gggcggcacc gtggcgcgga tggcctcgtt gaggttgtcc agggagaccg
     7501 gcaggaggcc ccgctggaac gcggcgccga tgaggaggac gttggcgtag agtttgttgc
     7561 cgaaataggt ctcggagacg ttggagaaat cgagccccag atagtcgtcg gcgcgggtgt
     7621 accgcttgac ggtgtcctcc aggtcctgcg gcgagaaatc atccttgccg aggagcgtgc
     7681 ggatggtggg gatcttgtgc gtgttcatca ccgccacggt ccgcgtgggg ctggccacgc
     7741 gcaggttcac tttcggatcg agcccccggg tggcttcaag gaggtcgatg ccgaggagaa
     7801 ggtcggcgtg gccgtaaggc acgagaggcg aaaggacctg ctcctcgcgg gagaaaatca
     7861 cgtggccgta cacgccgccg ttccggatgg cgaggccttt tttgtcggcg aagatggcgt
     7921 ggtatccctg tcggagcccg gcgcgcacca gcacgccggt gaccaccccc gcgcccgtcc
     7981 cgccgacgcc ggcggtgtag cagaaccacg tgtcggagac ttctttgagg ggaggcaggg
     8041 gcaatgaccc caccacggct tccggcaggg gcgccggcgg ctgacgccgt cggatggtga
     8101 tctcttcgaa ggaggggcag accttggcct tggtgcaggc ggcgtcggcg atgcagaggg
     8161 accggtccgt gacgaccttg ggcccgtgga gggtgttctc aatggccagg ccgggacatc
     8221 ccgtgtcgtt ggtgcattcg aggcagaatt cgcagacctc cggcgtggcg ttgatgactt
     8281 tttcctcggg gaggtagccc tggagccgga tgattttttt ccgttcccgt cggacacggc
     8341 ggtggtaggt gatgccgcac tccttgtcgg cgatgatgac cttgacgccg tccttgagga
     8401 tgactccctc catgagcgtg cgataggagt tgcggtaggc ggggttgacg cggtagacgg
     8461 ggatggagtc gcgggccatg ccccggatga cctgttcgat gtcctgcgcg aacgttttct
     8521 ggtagaggag gttgatggcg ttgccggggg tgggctggtg tccggtcatg gcggtggtct
     8581 tgttgtcgag gatgacgaag gtgatgtcct ggttgttctt gatggcgtcg gagacggcca
     8641 gcatgccgga gtggaagaag gtggagtcgc ccacgaagac gacgtgtttg tttttcacga
     8701 agggggagag tccggcgccg gtgccgccgc ccaggcccat gccggagtag tcctgcatga
     8761 ggcccaggtt cggctcgaac tggagcatgg aatggcagcc ggattctccg tggaagatga
     8821 catccagcgg gtcccggtcg tatttcttcc gcatgtaggc ggcatccagg aaatcccgtt
     8881 tcagggattt ggccaccgtg gaggaatcgc ggtgggggca ccccggacag aaggtgggcg
     8941 tgcgggcggg gattttgacg tcgaaggcgg cggtgtccgt gacggtgcgg tgcgcctttt
     9001 cccatcgcgc gccggcgtcc gacggcaggg ggaggagacg ttgcttgaag agtccgccga
     9061 ggcgttccat caggatggag gtgttgatcc cgcggatgga ggggatcccg tcctggtcgt
     9121 ccggaaattt cttgccccac accgcgggca tgcgtttcag gcggccggtc tgcaccgcgc
     9181 gctggaggag gtggaccatc tgtgtttcca cgaaggcgcg tttctcctcg atgacgtaga
     9241 tctggtccac gcgcgaggcg aattccatca ggatgtcctc ttccaggggg aacgtgacgc
     9301 cccatttcag gacggggaac cggttttcca ggcccatctc gcgcagggcc tgcatcaggt
     9361 agcaatacga gagcccggag gcggcgaacc cggcggagga tttttccccg gcggcgcccc
     9421 agtcgatccg gttcaatcct ttttggcgga ccagcgtttt caggcggtcg aaacgggccg
     9481 ccagcgtggc ctcgcgccag gaggtgtgcg gggggatcat caccgtgttc tcgacggcca
     9541 tgttcgccga ggagagggcg gtcttttgat tgttgttgac gacggggtag aggttgggcc
     9601 ggcagcgcac cgcgccgccg ccgtcggctt ggttggtggt gatgacgtac gtgatgtaga
     9661 gattggcctg gccggaaagt tcgaaaccgt ctttgatcca gtccttcagc tcctggaagg
     9721 tggccggttc caagaccggc atgttgaggt gttgggagag gaagcgggaa tcggagttga
     9781 tctgggtggt ctcgttccag ggatcgtcgc cgaccaccac gacggcgccg ccgtcgtggt
     9841 tctggggttc ggcgaggttc ccgatggcca gcccgtcggc ggccacgtgc aggccgacgg
     9901 atttcatgag gctcatggcg cgcaaattgg cgaggcgggc gccgttgagg cgggcggcgc
     9961 tgagggcctc gttgttggct atctgcgcca caatgcctcg ctccgtgaac aggtccgcga
    10021 cggaactggc ggcgtcgaac acctccgaga tgggggagcc gggataaccc gtcatgaggg
    10081 ccatgccgcc ttccagggcg cccttgagga gcagttcgtt gccggtgaag gcttccatgc
    10141 cgtcttcttt taagaatcgc ttgtccattg tggatccccc cgaaatcagc gttacgttct
    10201 cgacgtcgcc aagagttcct tggcgtgttt gacgctgatg gattcgtcct tggtcttttc
    10261 ggcggctccg ccgaacatcc gcgcgatctc cagcacgcgg ccctcttcct gcagtttctt
    10321 gatggtggtg gaagtgcggt ctttgacgac gtgcttttcc acgtagaaat ggttttgggc
    10381 gcaggccgcg atgggagcca ggtgggtgat gcacaacacc tggtgcgtct ttccgagccg
    10441 ggccagtttt ttcccgatga cgccgccgag gcggccgccg acccccgcgt cgatctcgtc
    10501 gaagaccagg gtggggacct cgtcggcccg cgccagcacc gatttcaggg ccagcatcac
    10561 gcgcgagagt tcgccgcccg aggcgatgtc ggccagcggc tggcgtcctt cgcccggatt
    10621 gggcgaaaag aggaatcgca ccgcgtcgaa acccgccgcg gtggcgggcc cgtcggggtt
    10681 tttttcgaca gccacgtcga aggcggcgtg gggcaggccg acctcatgga attccttttc
    10741 cacggcggcg gccagtttct tcgccgcggc ggtgcgggcc tggctgagct tgagacagac
    10801 gtccttgagt tcggattgcg ccttggcgag cttttcggag agatcgcggg tgcgttcctc
    10861 ggcgttttcc agatatttga gctcctcctc gatcttcgtc tgatgcgcca tcacgtcctt
    10921 cagcgcgggg ccgtatttgc gtttgagttt gccgaggacg tcgaggcgcg agaggacctc
    10981 gtccagccgt tccgggtcga ggtccacacg gttcttgaag gcttccagct catgggccgc
    11041 ttcttccagg cgcacggccg attcggcgat catgtccggg atggtgcgga ggtctcctcc
    11101 cagggactgg agcgtctcca cgtcgcgctg cacgcggcgc acgttgtcca gcgcggagcc
    11161 gtcgctcccg tagagaagat cgtaggattg ttgggaaagg ttcttgaggc gctcggcgtt
    11221 cttcagctgg gggaggaggc gttcgaggtc ttcgtcctct ccctcggttt tcagctgggc
    11281 ggtcttcaat tccgtcagct ggaaccggta gaggtccacc cgctgggcgc gttcctggtc
    11341 ggaaagagag acggcctgct gttcggcgag cagggccttc cagcgtccgt gggcctcggc
    11401 ggtggtttgg cggagcggct cgagccggcc gaaggtgtcg aggacgtcca gctgttccga
    11461 ggatttcagg aggagctggt gttcgtgttg gccgtggatg taggcgaggt gttcgccgat
    11521 gcgggcgagg gtggagaggt tgaccgggtt gtcgttgacg aaacagcggg agcggccgcc
    11581 ggcgtcgagt tcccgccgga ggaggagttg gtcgccctct tcggcggcga ggtcgagttc
    11641 ccgcaggagc cgtttcaggc gcggcgcgga gagacggaac tccgccacca cgctgaggcg
    11701 ggcggccccc tttcgcgcct ggctcgccga ggccttgtcg cccagcacca ggccgagagc
    11761 ctcgatgacg atcgttttac cggcgccggt ctccccggtg aggacgttca accccggggt
    11821 gaattccagg aacatgtcct cgatgagggc gaaattctta atggatagat agttcagcat
    11881 ggatttgtcc gtgcgccggc gtcgttggcg cggaaatcag cgttcccccc agcggagttt
    11941 ctgttgcagg agggagaaat agggcctttg cgggttgaga aaaagttgga accgttccgt
    12001 ggcgcgttcc acgcggatgc ggtcgccggt gcggagagga aaatccgtct ggccgtcgag
    12061 ggaaaggagc accttgtccc ttttggcccg cgcttccacc gagatgtcca aaacgctgtc
    12121 ggcggaaagg atgaccggcc gctgcgtcag gctgtgcgaa cagatcgggg tgaggagaag
    12181 aaggtccatt tccggctggg cgatggggcc ggaggcggcc agggaatagg cggtggagcc
    12241 ggtcggcgtg gcgaggatga gaccgtcccc cacgaaggtt ccgagatatt cgccgttcac
    12301 ccgggccgag aggcggatgg tgcggacggt ggagctgacc cggatgacgc agtcgttgag
    12361 ggccagccgg ggcttttggc gcggcgcggt gacgctgatc atcatgcggc tggagcagac
    12421 cagccggcct ttcagaaggg cctccagggt ctgatacatc cggtggagat cggtggcggc
    12481 caggaacccg aggtggccca gattgacgcc gagcgccggg atgccccagg gggccagccg
    12541 tcgagccgcc gacaggagcg tgccgtcccc tcccaaggcc accgccacct gcgcttcccg
    12601 cgcccgcgcg ggaggcacgg tgcggatccc gtgccgacgg aaccacgccg acaggcggga
    12661 cagttcccgg cgggcgagtt tcttggcggc gttatgaaag acgacgacac atttcggcgt
    12721 aggggtcatg aagggggcgt ttttcccgag cggggcctca ggaatttccg ttttttcgga
    12781 ccagttcttc ccagattttg aggatgttgc cgtcccacca gccgcggtcc acttcttcgt
    12841 gaaggatgcg cagcgctttg gccggagcga aggcctcttt gtaggaacgt ctcgaggtga
    12901 gggcgtcgta gatgtccacc atgctcacga tacggacgac gtcgggaatt ttggagcctt
    12961 tgatgccatc ggggtagccc gtgccgtcca ggcgttcgtg gtggtggcgg atgatgggga
    13021 tgatctcctg catcaccttg aggcggctgc agatgtcgca gccgatggtg gggtgtttgc
    13081 gcatggtttc ccattcgtcc ttgtcgagcg ggccttcttt ctggaggatg gcgtcgggga
    13141 ctcccagttt gccgatgtcg tgaaggatgc cgcctttgcg caggagttcc agttttcggg
    13201 tatccaagga aagggctttt ccgagcgcca gggcgaaccg gccgacgcgg tcggcgtggc
    13261 cgagcgtgta ctggtccttg gcctcgatgg tccgcgccag ggtgaagagc accacttcgg
    13321 cgtcttccag ttcgtccgtg agggatttca tccgcagcag ggacttgatc cgggccagga
    13381 gttcggcgtg ttgaatgggt ttggtgagga aatcgtcgca gccggcctcc agggcgcgga
    13441 gtttttcctg ttccggggag aggccggtca tcatgatgat ggggatgaaa cgcgt
//
LOCUS       DAOTFD010000051        13209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3259_length_13209_cov_4.825452, whole genome shotgun sequence.
ACCESSION   DAOTFD010000051 DAOTFD010000000
VERSION     DAOTFD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_13209_cov_4.825452"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /gene="yidC"
                     /locus_tag="P5079_04700"
     CDS             complement(<1..1292)
                     /gene="yidC"
                     /locus_tag="P5079_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRY29321.1"
                     /translation="MSKNLALAILLSFGILFAWDALVMRPRRQQMAPAAASPSTPQTV
                     SPPPAVGPAPATTAAPERLRLVEYLVGRQRVAFNLFGGGVNQWTFEEKDRSWIPLLPD
                     TGVAYRPFQVFPELEYTADSPRTGTVRLSAERSDGLQVEKSFRISSQTYIHEMALTLK
                     NRSKEPLTVSYDIGWGPGLESGDTDPKEKIRSQRALAFEAPRLHKIKEGTRTVPARWW
                     GLDARYFLAAFLSRGQNVHVSVDKINDHFVVKKSTTVELPPGASFSETLPFYLGPKGY
                     AQLASLREGLERSVDFGFFGGLGRIVLRILYYFHRLTHNYGWAIILLTLMVQILTIPL
                     TIHSFHHSQKMKAIQPQMKKIQELFKSDPKRLNAEMLNLYQRHGLKFMGMEGCFPILV
                     QLPVFWALYTTLRNAYELRGAPWIFWVRDLSVHDPFYIL"
     gene            complement(1285..1521)
                     /gene="yidD"
                     /locus_tag="P5079_04705"
     CDS             complement(1285..1521)
                     /gene="yidD"
                     /locus_tag="P5079_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRY29322.1"
                     /translation="MEHVLQTSLLFAVRAYWGVSSLFRPRCRFWPTCSHYLHDAVLIH
                     GSLLGLRLGFRRLLRCHPFGGSGFDPVPEKVAHV"
     gene            complement(1974..2162)
                     /locus_tag="P5079_04710"
     CDS             complement(1974..2162)
                     /locus_tag="P5079_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29323.1"
                     /translation="MLTYAEENISAPSSETEENDRFSGAHVHLGWAARIETATPKRPL
                     EVKPIESGPPASDGRARA"
     gene            2299..3324
                     /locus_tag="P5079_04715"
     CDS             2299..3324
                     /locus_tag="P5079_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29324.1"
                     /translation="MPDISIIIAAYNVQREIDVLLESLCRSRFRDFEICLCDDASSDD
                     TRRTVERYRDRLAIKLTVNPSNRGVTYSRNAALALAQRPLLLFLDADVRLSPDTIDRL
                     WAAMERTRADVVVGGYGSTALDPGIFSDYYALFVSQSFHVAHGPVPYNVFNAWCALCR
                     REVMSVTGGHVVVPKGVEIENESLGRRIVAAGFSLVLDPAIQVDHFWGGHRKLFFIFT
                     SRIYWWVKIFLATRCRFEIALTNSSYGFGTVCFPLAAAFGVLAFSVDPWFWIASAAAG
                     AFFVRAYAPFYGFVFRRRPLFVPMSVLLSAYFSFMITACAAYSFIEEVVRLVFARRGT
                     LDPALFR"
     gene            3338..4834
                     /locus_tag="P5079_04720"
     CDS             3338..4834
                     /locus_tag="P5079_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29325.1"
                     /translation="MKKIVLCYSRNNPYSYDAHTPLSILSLGTFLETKGVEVDYFDER
                     VQPIGRFHELLAQKPALVGFSVIGGYQIASAARLSRAVRRDSPASLIVWGGLAPTTLP
                     KETAAEDFVDAAVVGEGEQTLLELVRGLGAGALELSSVAGLVFRRDGGIVVNPPRPAM
                     DMEELPFVYQGKAREMLRLYLRRKNVREMTGYETSRGCPFFCRFCYSPHFNRGNVRVK
                     SLGKIAEELRSIRELGVDDFDVYDDTLLGGRQEEIPAFVDRLGRTGFRWIGNLRINMV
                     SDGLIDKLEKSGCRWVYFGLESNDDRVLAEMRKGITSEQIRTGVEVMRRHRVSTVYSI
                     VMGLPLESEDMDVNKYLDFADRLHALHPSAEIQLQSYVPLPGTDLYAASLRRGFRPPV
                     RLLDWAELDHFGVTNPWIRPASLPRRLYLSSFLAYRYKRHLAHFPLGLFFYPFHRLAL
                     FRFRRRWFGFFMEDYFYFAALFAAKALTSLRFFLRDGGGRFGGEPQAR"
     gene            complement(4822..6744)
                     /gene="asnB"
                     /locus_tag="P5079_04725"
     CDS             complement(4822..6744)
                     /gene="asnB"
                     /locus_tag="P5079_04725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY29326.1"
                     /translation="MCGIAGVLTLNSSVTPEDIVQVHRMTDILRHRGPDSRHVLHDDR
                     CALGNTRLKIIDLSSNADLPMTNDDRSVWLTYNGEITNYKELIHKFSLDKKYRFRSTS
                     DAEVLLRLYEEIGISLLDHVSGMFAFCLYDVRKAKAYVVRDFFGLRPLFFMAKENRFY
                     FASEIKSFLQLPAFRGELNHEAIYHFFSLAYIPGSLTPFKDVTEVRSGQLYEVDLKAG
                     TFQEREYYRVRYLPNDSLDERDTALRLHGVLLDAVRRNLISDAPLGLTLSGGFDTSSL
                     LALTKELYPEREVHTFSIKINEPSFDESRYQKIVVDYFKPVHHEIVVNPQDVADNLLR
                     HMAYLDEPTGDGAAIPSFLLSREAKKTVSVLLSGEGGDEVFNAYETHGAYKMRRLYRR
                     YAPPFLRRLLREGARRLPTSYNKLSLDFILKRFTEGAEKSVPESHLFWRHVLTDDEKR
                     RLLRRPPDDFRETVHYFSDMFDELDFADELNRISAIDMKYYFVDDLMVKNDRTFMAHS
                     VEARFPYMDRSVVEFVSRIPPEMRLKGLRRRHIQKEAMKPYLPKEIYRRTNMGLEMPH
                     SIWFMKSLKDFYGKYFTRENVGRSGLLDPAMVEGLWQDHFSRKRDNGRALWCVLNFMI
                     WFDMFVWKKDYKNYLA"
     gene            complement(6835..8346)
                     /locus_tag="P5079_04730"
     CDS             complement(6835..8346)
                     /locus_tag="P5079_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRY29327.1"
                     /translation="MKIVNPLLPGFHPDPSVCRVGHDYFLVTSSFEYFPGVPLFHSRD
                     LVHWRPIGHCLTRKSQLPLDRAAPSQGIFAPTLRFHNGRFYMVTTNVSDKGNFLVSAA
                     DPSGEWSDPVWLDQPGIDPSLFFDASGAVYLTSAGEDGILQSRIDIATGQRRTPLRTI
                     WRGTGGRYPEGPHLYKIDGRYYLMIAEGGTEYGHMETVARAGSPWGPFEPCPDNPLLT
                     HRHRGRHPIQATGHGDLVQSPDGSWWMVFLGIRPVDEHYHHLGRETFLAPVVWTKNGW
                     PRVTTGGTVEMTMDGPAGPPCRWPREPERDDFDSPRRRLCWNFLRNPDEKSWSLTDRP
                     GWLRLTGSGTTLDEAASPSFIGRRQQHFFCEVAARMDYAPRSPREEAGLTAYMNERHH
                     YDIAVTHRGGRRCVLLRERIGDLRAVVSEKTAGAGPLTLRLHASPREYRFSFDAGRRR
                     NVLLGTAPTRYLSKEVAGGFTGVYFGLYAAGRGRPAFFDWFDYRPRRGSAGVI"
     gene            complement(8393..10282)
                     /locus_tag="P5079_04735"
     CDS             complement(8393..10282)
                     /locus_tag="P5079_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HRY29328.1"
                     /translation="MRWKALIPTVVVLSLIGVFTVFFLDGAVKCALEAAGTRMNGAKV
                     ELDDVNINLRKMSVTLRGLQVADPDSPMINTLDVESLRFDLAAKPLAWKKIIVENAEI
                     IGLATGTPRKSSGAVSKKKKPATEERPADKDEPSALAEKGQETFSFAMANLKEQYDPK
                     KLVQLENLASYQKIKGEQDRLPALTQGWESKADAVNLEAPSDRVKAFVQRVKSENFSG
                     AEGLKKAKDILSEGKKLKADLEQTKKQLAELKASVMAEVSQAKNALKEIDKLRQQDMD
                     NALGQVKSGFSAEGITRGLIGPEWFGKIQKALGWFHKIRKIIPQKKEEETPPPPDRRG
                     RDIHFPFRYNWPAFHLKNAGLSGVTAGTDPLTYKGTLKDVTSDPKKVGKPIALEISGK
                     SEAGPQSLSLRGEFDYTTDTPREQVRLRYAGIGLADTKLGQVGAPVSIREGNGVVTAD
                     LTIKAEKIAGDIRFEAAPVVLEQTLSAEQKKNRLLQILHDVLTTLDRLNVTVAVSDTL
                     TSPDFKLSTNVDDAVKNAVKQVLSKELEELKASFRARVSELVDGEKQKLTSQLDGKTG
                     GITDKFNKKDQLLQSAQGQIDQTLEDLKTKGQSALPIPGTGAPADAGESKPALPDLKG
                     IFKKR"
     gene            complement(10287..10796)
                     /locus_tag="P5079_04740"
     CDS             complement(10287..10796)
                     /locus_tag="P5079_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HRY29329.1"
                     /translation="MITIKLILKLINILNKEASPRQIAAGIALGSIAGITPTASLHNL
                     PVLVLILLLRVNISSAFVSLAAFSAFSYFLDPLFNKIGYALLTLPALKHFWTELYNTP
                     LLAWTNFNNTLTLGSLVFALLLFGPLYFVLAWAVKTYREKFLEKVRTWKIVQMLKASK
                     LYGLYASYS"
     gene            complement(10860..12416)
                     /locus_tag="P5079_04745"
     CDS             complement(10860..12416)
                     /locus_tag="P5079_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY29330.1"
                     /translation="MGKHLYRYPDPRLPADPGDFGDVWCLGDSHTQGAGAPPGMSYPQ
                     QLEGLLSARQPTHVLNLGYGGTNTSQQLHRLRAALSRATPKTLIYMGGINNGWKWSGV
                     DWKTLYAAHGPRGVAQSFLQRSRTYKFLFNHLLAGPGRQNISPPPVSPEDASEALMVF
                     QQPDAVLQTLGRRAVLSDDERFLLATAQFLSHKYPEALANFRAVAPLYRRRPLVVPID
                     LLIAETRRLAEAGPPSEETVRFYSTNPPAPDRKGRVCFLLGKGWMEIGRGNPQKSREF
                     FKNILQKVPDGYPFSFLFVETLGWSELLSGDLRAARAHFKSLEDRPPFLPLEFPFWSH
                     LGSARIGLIQQDAVLYSAERSIVKNYAEKNPYYRLWADELPDPEHLSPEMIQALNFSL
                     PRLSLLHHLPPNKWAVFKNSLPAATVLTQDLDALAGLARKHGFRLVLMGYPFANYWAN
                     DVLRDFAMDQGIPFIDLHRELGDFSRTGHLSADRGHPNQEGYRRIAETVRRRLSLLDA
                     PGEPVAGDTH"
     gene            complement(12549..>13209)
                     /locus_tag="P5079_04750"
     CDS             complement(12549..>13209)
                     /locus_tag="P5079_04750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY29331.1"
                     /translation="SVNVTAFEKILDHALSTGCPKVVYASSAATYGKGAVPMREDQPP
                     APANVYGESKVEMDKVARAYVRKNPNLSIVGLRYFNVYGPLEFHKKSAASMIYQLYQQ
                     IAAGKPPRVFKWGEQYRDFIYVKDVVQANMKAFAFRGSGIFNTGTGLPTTFNQVISLL
                     NKALGADRPTDYFDNPYNFYQEATQADPALAEKTIGFKAAYPPEKGIPDYVRFLKSAA
                     R"
BASE COUNT         2351 a         3685 c         4447 g         2726 t
ORIGIN      
        1 aggatgtaga agggatcgtg gaccgacagg tcgcgcaccc agaagatcca cggggcgccg
       61 cgcagttcgt aggcgttgcg cagcgtggtg taaagggccc aaaagaccgg cagctgaacc
      121 aggatgggga agcatccctc catgcccatg aacttcaggc cgtggcgctg gtaaaggttc
      181 agcatctccg cgttcaggcg tttggggtcg gacttgaaga gttcctggat cttcttcatc
      241 tgcggctgga tggccttcat cttctggctg tgatggaaac tgtggatggt gagcgggatg
      301 gtcaggattt ggaccatgag ggtcaggagg atgatggccc agccgtagtt gtgggtgagg
      361 cggtggaaat aataaaggat gcggagcacg atgcggccca gcccgccgaa gaacccgaaa
      421 tccacggaac gttccagccc ctcccggagg gaggccagct gggcgtaccc tttggggccg
      481 aggtagaagg ggagcgtctc ggagaaggac gccccggggg gcagttcgac ggttgtggac
      541 tttttgacca caaaatgatc gttaatcttg tcgacggaga cgtggacatt ttgtcctcta
      601 ctgaggaaag ccgccaggaa gtagcgggcg tccagccccc accagcgggc ggggaccgtc
      661 cgcgtccctt ccttgatctt gtgcaggcgg ggcgcctcga acgccaacgc ccgctgggaa
      721 cggatcttct ctttggggtc cgtgtcgccc gattccaggc ccggccccca gccgatgtcg
      781 taggataccg tcagcggttc tttggatcgg ttctttaaag tgagcgccat ttcgtggatg
      841 taggtctgcg aggagatgcg gaaactcttt tccacctgca acccgtcgct ccgctcggcc
      901 gagagccgga cggtgcctgt ccgggggctg tcggcggtat actccagctc cggaaaaacc
      961 tgaaagggtc gataggcgac gccggtgtcg ggcaggaggg ggatccagct ccggtccttc
     1021 tcttcaaagg tccattggtt cacccctccg ccgaagaggt tgaaggcgac gcgttggcgg
     1081 ccgaccagat attccaccag ccggaggcgt tccggcgcgg ccgtcgtcgc cggcgcgggg
     1141 ccgacggcgg gcggcggaga gaccgtttgc ggtgtcgagg gggatgcggc ggccggggcc
     1201 atctgttgcc gccggggccg catgaccagg gcgtcccacg caaagaggat gccgaacgac
     1261 aaaaggatgg ccagggcgag gtttttagac atgggcgacc ttctccggca ccggatcgaa
     1321 ccccgacccg ccgaagggat ggcagcggag gagtcgtcgg aaccccagcc ggagcccaag
     1381 gagggagccg tggatcagga ccgcgtcgtg aaggtagtgg gaacaggtcg gccaaaaacg
     1441 gcagcggggc cggaaaaggg acgatacgcc ccaataggcg cgcacggcaa aaagaaggga
     1501 tgtctgaagc acatgttcca taaggttttt cttaactccc gggggacgag tcagggcagg
     1561 ggacgaaacg cggtcagcgg gtttctgttt ggcgtttttt cgcggacggc ggatgaagcg
     1621 ccggtttcaa agcagccgcg aattcgagaa aatcacgcaa aagatcccgc tggtcaggcg
     1681 gaaaattgtc ccttgtcttc ggcgaaatga ggatgtccca tccccgcggg agtccgtgtt
     1741 tgttgagacg gaaaacctcc cgtattcgcc gtttgaaaag attccgttgc accgccggtc
     1801 cgaaccgccg gcccaccgcc acgcccaggc gcgggaggcg gggtggttct tccgggcgcc
     1861 gataacccca ggcgaccatc gcccggccga cccgtttccg gccgtctttt cggacccgaa
     1921 ggaaatcttc gcgccggcgc agccgctcgt gagcgcgaaa agtgaaccgc atgtcaggct
     1981 ctggcccgcc cgtcggacgc cggcgggccg gattctatgg gtttaacttc cagcggcctt
     2041 tttggcgtcg ccgtttcaat acgcgccgcc cacccgaggt ggacatgcgc gcccgaaaac
     2101 cgatcgtttt cttccgtctc cgatgatggg gctgaaatgt tctcttcggc ataggtcagc
     2161 attctatctt ttgcccgtct caagcgcaac ctggacgatg aaaaaagaaa aggcttccgg
     2221 gtcctcaaaa taggttgaag gggccgtaat gaaagaaata atatgtagca gaccgcatgc
     2281 gagaaaaagg atccgtccat gcccgacatc tccatcatca tcgccgccta taacgtccaa
     2341 cgggaaatcg atgtcctttt ggagtccctg tgccgctccc gcttcaggga ttttgaaatt
     2401 tgcctctgcg acgatgcctc ttcggacgat acccgtcgca cggtggagcg gtatcgggac
     2461 cggctcgcca tcaaactcac cgtcaacccg agcaaccggg gggtgaccta ttcccggaac
     2521 gcggcgctgg cgctggccca gcgccccctc cttctttttt tggacgccga cgtccggctc
     2581 tccccggaca ccattgaccg cctgtgggcg gccatggaac ggacccgggc ggacgtggtg
     2641 gtggggggct acggctccac cgcgctcgac cccgggatct tttccgatta ctacgctctt
     2701 tttgtgagtc agtcgtttca tgtggcccac gggcccgttc cctacaacgt cttcaacgcg
     2761 tggtgcgccc tctgccggcg ggaggtcatg tccgtcaccg gagggcacgt ggtggtcccc
     2821 aagggggtgg agatcgagaa cgaatccctg gggcggcgca tcgtggccgc ggggttttcc
     2881 ttggtgttgg acccggccat ccaggtggac catttttggg gagggcaccg gaaattgttc
     2941 ttcatattca ccagtcgtat ttattggtgg gtcaagattt tcctggccac ccggtgccgt
     3001 tttgagatcg cgctgaccaa cagttcctac gggttcggga cggtgtgctt cccgctggcc
     3061 gcggcgttcg gcgttctggc tttttccgtg gatccctggt tctggatcgc gtcggccgcg
     3121 gccggggcgt ttttcgtgcg ggcctatgcg cccttttacg ggttcgtctt ccgccgccgc
     3181 cccctcttcg tgccgatgag cgttctgttg agcgcctact tttcgttcat gatcaccgcc
     3241 tgcgccgcct attcctttat agaggaagtg gtccgcctgg tttttgcccg tcgcgggacg
     3301 ctggatcccg ccctgttccg atgacgccgc gccgcccatg aagaagatcg tcctgtgcta
     3361 ttcccgaaat aacccctact cctacgacgc ccatacgccg ctctccatcc tgtccctggg
     3421 gactttcctg gagacgaagg gggtggaggt cgattatttc gacgaacgcg tgcagccgat
     3481 cgggcgtttc cacgaactgc tcgcgcagaa gccggcgctg gtcgggtttt ccgtcatcgg
     3541 cggttatcag atcgcgtcgg ccgcccggct ttcccgcgcg gtccggcggg attcccccgc
     3601 ctccctcatc gtctggggcg gactggcgcc gaccacgctg ccgaaagaaa cggcggcgga
     3661 agattttgtg gatgccgccg tcgtcggaga gggggagcaa accctgctgg aattggtccg
     3721 tggtctcggg gcgggggccc tcgagctctc ctcggtcgcg ggcctcgttt ttcgccgcga
     3781 cggcgggatc gtggtgaatc ctccgcgtcc ggccatggac atggaagagc tcccgttcgt
     3841 ctaccaggga aaggcccggg agatgctgcg gttgtatttg cgtcggaaga acgtgcggga
     3901 aatgacgggg tatgagacct cccgcgggtg cccgttcttc tgccggttct gctacagccc
     3961 gcatttcaat cgggggaacg tgcgggtcaa atcgctgggg aagatcgcgg aggaactgcg
     4021 gagcatccgc gagttggggg tggacgattt cgatgtgtac gacgacacct tgttgggcgg
     4081 ccgccaggag gagatcccgg cgttcgtgga ccgtttgggt cggaccggtt tccgatggat
     4141 cgggaacctg cgcatcaaca tggtgtcgga cggcctcatc gacaagctgg aaaaatccgg
     4201 ttgccgatgg gtctatttcg ggctggaatc gaacgacgac cgcgtcctgg cggagatgcg
     4261 gaagggcatc acgtcggaac agatccgcac gggcgtggag gtgatgcgcc ggcaccgggt
     4321 gtccaccgtc tattcgatcg tcatggggct tccgttggag agcgaggaca tggacgtgaa
     4381 caaatatctg gatttcgccg accggctcca cgcgctccat ccgtcggcgg agatccagct
     4441 gcagtcctac gtgccgctgc ccgggacgga cctgtacgcg gcctccctcc gccgggggtt
     4501 ccgcccgccc gtccggcttc tggactgggc ggaactcgac cacttcggcg tcaccaatcc
     4561 ctggatccgg ccggcctcgc tcccccggag actttacctg agttctttcc tggcgtatcg
     4621 gtataaacgg cacctggcgc attttccgct gggcctgttt ttttatccgt tccaccggct
     4681 ggcgctgttc cggttccgcc ggcgctggtt cggttttttc atggaggatt atttttattt
     4741 cgcggcgctc ttcgcggcga aggcgctcac gtcgctgcgc ttctttttgc gggatggcgg
     4801 ggggcggttc ggcggggaac ctcaggccag gtagtttttg tagtccttct tccaaacgaa
     4861 catatcgaac cagatcataa aattcaacac gcaccacagg gcgcggccgt tgtcccgttt
     4921 gcgggagaaa tggtcctgcc agagtccctc caccatcgcc gggtcgagga gaccggaccg
     4981 ccccacgttc tcgcgggtga aatatttccc ataaaaatcc ttcagcgact tcatgaacca
     5041 gatggagtgg ggcatttcca gccccatgtt cgtccgccgg tagatctctt tcggcaggta
     5101 gggtttcatg gcttctttct ggatgtgccg ccgacggagc cccttgagcc gcatctccgg
     5161 cgggatgcgg ctgacgaact ccaccacgga ccggtccatg taggggaaac gggcctccac
     5221 ggagtgggcc atgaaggtgc ggtcgttttt caccatcagg tcgtccacga aataatattt
     5281 catgtcgatg gcggagatgc ggttcagctc gtccgcgaag tcgagttcgt cgaacatgtc
     5341 ggaaaaataa tggacggtct cgcggaagtc gtccggggga cgccgcagga gccggcgttt
     5401 ctcgtcgtcg gtgagcacgt gccgccagaa aagatgggat tccggaacgc tcttctccgc
     5461 cccctccgtg aaacgcttca atatgaaatc gaggctgagc ttgttgtagg acgtgggcag
     5521 ccgccgggcc ccctcgcgca gcagccgccg gaggaaggga ggggcgtagc ggcggtagag
     5581 gcggcgcatc ttgtaggcgc cgtgcgtctc atacgcgttg aaaacctcgt cgccgccttc
     5641 gccggacaac aggacggaga ccgttttctt cgcctccctc gaaaggagga aggacgggat
     5701 ggcggcgccg tcgccggtgg gttcgtccag gtaggccatg tggcggagca agttgtcggc
     5761 cacgtcctgc gggttgacca cgatctcgtg gtgcaccggc ttgaagtaat ccacgacgat
     5821 cttctggtag cgggactcgt cgaaactcgg ctcgttgatc ttgatggaga aggtgtggac
     5881 ctcccgttcg ggatagagtt ccttggtcag ggccaggagg gaactggtgt cgaacccgcc
     5941 ggaaagcgtc aaccccaggg gagcgtcgga gatgaggttc cgccgcaccg cgtccagaag
     6001 gaccccgtgc agccggaggg cggtgtcccg ctcgtcgagg gagtcgttgg ggaggtagcg
     6061 gacccggtaa tattcccgtt cctggaacgt ccccgccttg aggtccacct cgtagagctg
     6121 cccggaacgg acctcggtca cgtccttgaa gggcgtaagg gagccgggga tataggccag
     6181 cgagaaaaag tggtagatgg cttcgtggtt caactcgccg cggaaggcgg ggagctggag
     6241 gaatgatttt atctcggagg cgaagtagaa ccggttttct ttcgccatga aaaaaagcgg
     6301 ccgcaggccg aagaagtccc gcaccacgta ggccttggcc ttccggacgt cgtaaagaca
     6361 gaacgcgaac atgccgctga cgtggtccag aagggagatg ccgatctcct cgtagaggcg
     6421 cagaagcacc tcggcgtcgg aggtggagcg gaaccggtat ttcttgtcga gggagaattt
     6481 gtggatcagt tccttgtagt tggtgatctc tccgttgtag gtcagccaga cggaacggtc
     6541 gtcgttggtc atgggcaggt cggcgttgga ggaaaggtcg atgattttca ggcgggtgtt
     6601 gcccagtgcg catcggtcgt cgtgcaggac gtgccgggaa tccggcccgc ggtgacggag
     6661 gatgtccgtc atccggtgga cctgtacgat gtcttccggg gtgacggaag aattaagtgt
     6721 cagaacgccc gcgatgccac acatgaagag caggtctcct taaggaagcc cgatgcgtcg
     6781 aatcgaccgg tgaccgctga accggaagga ttttcccttt tccggaagga ttggtcaaat
     6841 aacgccggcc gatccgcggc gcgggcgata gtcgaaccaa tcgaaaaacg cgggccggcc
     6901 ccggccggcg gcatacagac cgaaataaac cccggtgaat ccgccggcca cctctttcga
     6961 aagatagcgc gtgggggccg ttcccaaaag gacgttcctc cgacggccgg cgtcgaagga
     7021 gaaccgatat tctcgcggcg aggcatggag gcggagcgtg agcggacccg ctccggcggt
     7081 tttttccgaa accacggccc ggaggtcgcc gatccgttct cgcaacagga cgcatcgtcg
     7141 gccgccgcgg tgcgttaccg cgatgtcgta gtggtgccgc tcgttcatgt acgccgtcag
     7201 gccggcctct tcccgcggcg accgcggagc gtagtccatc cgggcggcga cttcgcagaa
     7261 aaaatgttgc tgccgccggc cgatgaaaga gggggaggcc gcctcgtcga gcgtcgtccc
     7321 ggatccggtc agccgcagcc agcccggccg gtccgtaagg gaccaggatt tctcatccgg
     7381 attccgaagg aaattccaac aaagccgcct tcggggggag tcgaagtcgt cccgttccgg
     7441 ttcccggggc caccggcacg gggggccggc gggcccgtcc atcgtcattt cgacggttcc
     7501 gccggtcgtc acccgcggcc aaccgttttt cgtccacacc accggggcca ggaacgtctc
     7561 gcggccgagg tggtggtagt gctcgtccac cggacggatg ccgaggaaga ccatccacca
     7621 ggagccgtcg ggcgactgaa cgagatctcc gtggccggtg gcctggatgg ggtggcggcc
     7681 gcggtggcgg tgcgtcagga gggggttatc gggacagggc tcgaacgggc cccacggcga
     7741 accggcccgg gccacggtct ccatgtgccc gtactccgtg ccgccttcgg cgatcatgag
     7801 gtaatagcgg ccgtcgatct tgtagaggtg cggcccttcc ggataacggc cgcccgtgcc
     7861 gcgccagatc gttcggagag gcgtccggcg ttggccggtg gcgatgtcga tgcgggattg
     7921 gaggatcccg tcctccccgg cgctggtcag gtagaccgct ccggaggcgt cgaagaaaag
     7981 ggacgggtcg atgccgggtt ggtccagcca gacggggtcc gaccattctc cggaggggtc
     8041 ggccgcggag acgaggaaat tccccttgtc cgagacgttg gtggtgacca tgtagaagcg
     8101 gccgttgtgg aaacggaggg tgggggcgaa gatgccctgg gaaggggcgg cgcggtccag
     8161 cggaagctgg cttttccgcg tgagacagtg gccgatgggc cgccagtgga ccaggtcgcg
     8221 gctgtggaag agaggaactc cgggaaaata ttcgaaactg ctggtgacga ggaagtagtc
     8281 gtgccccaca cggcacacgc tggggtcggg atggaaaccc ggaagaaggg ggttgacgat
     8341 tttcatgggg cgtgggggcc ggaagacggt ctgggcggcc cccgtgggtg gattatcttt
     8401 tcttgaagat ccctttgagg tcggggaggg cgggtttgga ttcgccggcg tccgccggcg
     8461 cgcccgtccc gggaatcgga agagccgact ggcccttggt tttcaggtcc tcgagggttt
     8521 ggtcgatctg gccctgagcg gactgcaaaa gctggtcttt tttgttgaat ttgtcggtga
     8581 tgcctcctgt ttttccgtcc agttgggagg tgagtttctg tttttcgccg tccaccagtt
     8641 cgctcacgcg cgcccggaag gaggccttca gttcctccag ctctttcgac aagacctgtt
     8701 tcacggcgtt cttaacggca tcgtccacgt tggtggaaag cttgaagtcc gggctggtga
     8761 gggtgtccga gaccgccacg gtcacgttca ggcggtccag cgtggtcagc acgtcgtgca
     8821 ggatctgcag aaggcgattc tttttctgtt cggcggagag ggtttgttcc aagaccaccg
     8881 gggcggcctc gaaccggata tcgccggcga ttttttccgc cttgatcgtc aggtcggccg
     8941 tcacgacgcc gttgccctcc cggatggaca ccggggcccc cacttggccg agtttcgtgt
     9001 ccgccaggcc gatccccgcg tatctcaacc ggacctgttc cctcggggtg tcggtggtgt
     9061 agtcgaactc tccccgcagg gaaagactct gcggccccgc ttcgcttttt ccggagattt
     9121 ccagggcgat gggctttcct acttttttcg ggtccgaggt cacgtctttc aaagtgcctt
     9181 tgtaggtcag cggatccgtg ccggcggtga ccccgctgag tccggcgttt ttcaggtgga
     9241 acgcgggcca attgtagcgg aacgggaaat ggatgtcgcg gccgcgccgg tccggcggcg
     9301 gaggggtttc ctcctctttc ttctgaggga tgattttccg gatcttgtgg aaccatccca
     9361 acgctttctg tattttgccg aaccattccg gtccgatgag tccccgggtg atgccctcgg
     9421 cgctgaaccc gctcttgact tggcccaggg cgttgtccat gtcctgttgc cggagtttgt
     9481 cgatctcctt gagggcgttt ttggcctggg acacctccgc catgacggag gccttcagtt
     9541 ccgccagctg tttcttggtt tgttcgaggt ccgccttaag ttttttgcct tcggagagaa
     9601 tatccttggc ttttttcagg ccttccgcgc cggaaaaatt ttcggatttg acccgctgga
     9661 cgaaggcttt gacccggtcc gatggggcct cgaggttgac ggcatccgct ttggattccc
     9721 agccttgggt gagggcgggg aggcggtcct gttccccctt tatcttctga taggaagcca
     9781 ggttttccag ttggacgagt tttttcggat cgtattgttc tttcaggttg gccatggcga
     9841 aagaaaaagt ctcttggcct ttttccgcca gggcggaggg ttcgtccttg tccgcgggcc
     9901 gttcctctgt ggccggtttc ttcttcttgg acaccgcgcc gctggacttg cgcggcgtgc
     9961 ccgtggcgag gccgatgatc tccgcgttct cgacgatgat ttttttccag gccaacggtt
    10021 tggcggccag atcgaaacgc agggattcca cgtcgagcgt gttgatcatg gggctgtccg
    10081 ggtcggccac ctgcaggccg cgaagggtca cgctcatttt acggagattg atgttgacgt
    10141 cgtccagttc cactttggcg ccgttcatcc gggttccggc cgcctcgagg gcgcatttca
    10201 ccgccccgtc gaggaagaaa acggtgaaga ccccgatgag ggagaggacg acgaccgtgg
    10261 ggatgagggc tttccagcgc atgggtttag gagtagctgg cgtagaggcc gtagagtttg
    10321 gaggccttca gcatctggac gatcttccag gtgcgcactt tttccaggaa tttctcgcga
    10381 taggttttga cggcccaggc caggacgaaa tacagaggtc cgaagaggag cagggcgaac
    10441 accagactgc ccagggtgag cgtgttgttg aagttcgtcc acgccaagag gggggtgttg
    10501 tagagttccg tccaaaagtg tttgagggcg gggagggtga gaagggcgta tccgatcttg
    10561 ttgaaaagag ggtccagaaa ataggagaac gcggagaagg cggccaggga cacgaaggcc
    10621 gaactgatgt tcacccgcaa caggaggatc aggaccagga ccggcaggtt gtggagactg
    10681 gcggtggggg tgatgccggc gatggagccc agggcgatgc ccgccgcgat ctggcgcgga
    10741 gaggcctctt tattaaggat gttgatgagt ttaaggatga gtttgatggt gatcatgcaa
    10801 tttcctgatg cggtattcta gggtaatttg tttcgttcgt caattcgtcc gttgagaaat
    10861 caatgcgtgt cgccggcgac gggttcgccg ggggcgtcga gaagggagag gcgtcgccgg
    10921 accgtttcgg cgattcgacg gtagccttct tggttcggat ggccgcgatc ggcggacaaa
    10981 tggccggtcc gggaaaaatc gcccagttcc cggtgcaggt cgatgaaggg gattccctgg
    11041 tccatcgcga aatcgcgaag gacgtcgttg gcccaataat tcgcaaaggg atatcccatc
    11101 aaaacgagac ggaacccgtg tttccgggcc aaaccggcca gggcgtcgag atcctgcgtc
    11161 agcaccgtgg ccgcggggag gctgttttta aagaccgccc atttgttcgg ggggagatgg
    11221 tgaagcaagg agaggcgggg gagggaaaaa ttcagggctt gaatcatttc cggcgagaga
    11281 tgttcggggt cgggcagttc gtcggcccag aggcggtaat aaggattttt ttcagcgtaa
    11341 tttttcacga tcgaacgttc ggcggaatag aggacggcgt cctgttgaat gaggccgatc
    11401 cgtgcgctgc ccagatgcga ccagaacggg aattcaagcg gaaggaaggg ggggcggtct
    11461 tccagacttt tgaaatgggc ccgggccgcc cgcaggtccc cagacaggag ttcgctccac
    11521 cccagggttt caacgaataa aaaggaaaaa ggatacccgt cgggcacttt ttgaaggata
    11581 tttttaaaaa attcccggct tttttgcggg ttcccccgtc cgatttccat ccaccccttc
    11641 cccagaagga agcagacccg cccctttcgg tcgggcgccg gcgggttggt cgaatagaac
    11701 cggacggttt cttccgacgg cggtcccgct tccgccaacc gccgcgtttc ggcgatgagc
    11761 aggtcgatgg ggacgaccaa cggccgccgg cgataaaggg gggcgaccgc tcgaaagttg
    11821 gccaacgctt cgggatattt gtgggacaga aactgggccg tggccagcaa aaaacgttcg
    11881 tcgtcggaaa ggaccgcgcg ccggcccagg gtttggagga cagcgtccgg ttgttgaaaa
    11941 accatcaggg cctccgaggc gtcctctggg gacaccggag ggggggagat gttttggcgc
    12001 cctggcccgg cgaggagatg gttgaacagg aatttgtagg tgcgggatcg ctgcagaaac
    12061 gattgcgcca cgccgcgcgg gccgtgcgcg gcgtacaagg ttttccagtc caccccgctc
    12121 cacttccagc cgttgttgat gccccccata tagatgagcg ttttcggcgt ggcgcgggaa
    12181 agggcggccc ggagacgatg cagttgttgg gaggtatttg ttccgccgta gcccaggttg
    12241 aggacgtgtg tgggttgtcg tgcggacagg agcccctcca gttgttgcgg gtaactcatg
    12301 ccgggcgggg ccccggctcc ctgggtgtgg gaatcgccga gacaccagac gtcgccgaaa
    12361 tcgccgggat cggcgggcag tcggggatcg ggataacgat agaggtgttt tcccaaaagc
    12421 cacagagatg tttctgaaag tagaaggaga agggccagga gcgaaagcgt cgtccaaaga
    12481 atttttccga tgaggagacg ctttgccggc gggccggaag ggggttttgg caagaggggg
    12541 aaggggagtt atcgggcggc ggattttaaa aagcgcacat agtccgggat ccctttttcc
    12601 ggcggatagg cggccttgaa cccgatcgtc ttttccgcca gggcggggtc ggcctgggtg
    12661 gcttcctgat aaaaattgta cgggttgtcg aaataatccg tgggccggtc ggcgcccagg
    12721 gccttgttga ggagggagat cacctggttg aaggtggtgg ggaggccggt gcccgtgttg
    12781 aaaatgccgc tcccccggaa ggcgaaggcc ttcatgttgg cctggacgac gtccttcacg
    12841 tagatgaaat cgcggtattg ttcgccccac ttgaacaccc gcggcggttt cccggcggcg
    12901 atctgttggt agagctggta gatcatgctg gccgcggatt tcttgtgaaa ttccaggggg
    12961 ccgtacacgt tgaaataccg caggccgacg atggaaaggt tcgggttctt tcggacatag
    13021 gcgcgcgcaa ccttgtccat ctccactttg ctctcgccgt agacgttggc cggcgccggc
    13081 ggctggtctt ctcgcatggg cacggcgccc ttgccgtagg tggcggcgct ggaggcgtag
    13141 accaccttgg ggcatcccgt cgacaaggcg tggtcgagga tcttttcgaa cgccgtgacg
    13201 ttcaccgac
//
LOCUS       DAOTFD010000052        13207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3261_length_13207_cov_3.786648, whole genome shotgun sequence.
ACCESSION   DAOTFD010000052 DAOTFD010000000
VERSION     DAOTFD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3261_length_13207_cov_3.786648"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="P5079_04755"
     CDS             <1..1758
                     /locus_tag="P5079_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29332.1"
                     /translation="DWWTTYTVSPVGNGITYAVFKVLGVNHGTLRLAPAILGCLSVAF
                     LFWLAFRYAGPRAALFALLFSGVNVGLVAYARLAMLESFLLFFQTWLYVLCARWLFTR
                     RGAFWIGVLFVAAFGAKASAILFLIPLALVLFLFSAADRSVGRSGSVKGFVLGGGAAS
                     FLYLLFAAVNWPFFYHLNMGFSAHKTILDRSSGLLKVLELPTGNGELLLTLMPPLLWA
                     AWLGMGRLWNGEGLSALWESAGPPRQRIRGALERIARLDPFPRWALFCVLVFIGDLLV
                     HLFLRMDVSARRLLHMLPPMILTASLGLDRWLSGEGRPSRGGFFGVFSAFLVGWVAVK
                     ATVFCFFGVSGWSNPATFLFTRPFPVFRTPGDQSLFLGRGWFEMGYALVFAGAWLLWK
                     YRRARPGSAPERTEGLPGGRWKPVVAWTVAVVYLGHQLSMVLPPLLKPTYLYRDASRA
                     IERFSERPGAVVLGNMAAGLPWEGSYYPLYYMMSAGIGYKKKTMEDMGRIVDKYRPTL
                     MVVFDPDGKVGDGALYDWSSPDAVDEETRRTMEKHSPRIFDFREIVYRWKGLEGLYPR
                     GQWLLLKRKISEPASRLKK"
     gene            1755..3314
                     /locus_tag="P5079_04760"
     CDS             1755..3314
                     /locus_tag="P5079_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29333.1"
                     /translation="MTPLYDAPRPDILFAMSPAHARDMYMPIYFLYLAGYLEKKGYRT
                     EIVDPHRRGAEDNIRIILDRVAARRPRYVGLSSFATDYGCAVELARRIKEISDAKVVV
                     GNAQPSLTPEDYLFDGSPFDIVVRGEGELTLEEILSRGPAGGDLSGVRGIAYRENGRL
                     VLTEKRELMDLALCAMPAYHKVDMRWYRRATKHIIRRLFTVGAVVYTGRGCPFRCSFC
                     AANSVWQTNRITTAHPMVRKRPLPHVMAELRLLQDTYGFDFFYILDDTFGIHEKDITD
                     FCEAYRASGLRMLWAAETRVNCLKNDAIFKTLKSAGCLQLDFGVESGSQKLLNAICKG
                     INVPQVVEAFDLCRRNGIRTFANILLNLPGEDENDLVLTEQLLEKIRPDYISIGVTQP
                     YPGTAISRGLSRPLTRDDYAELSRLIPSEKFRLSKHNRDLYKLVSAWLLKYRAIAPFD
                     RSVLRADASYWKALLASNNKLNYAYFILRDLVTYPVVFAIFLIRRIGLLWRPSHVPPW
                     ADPMKEKDPGQ"
     gene            3671..4333
                     /locus_tag="P5079_04765"
     CDS             3671..4333
                     /locus_tag="P5079_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY29334.1"
                     /translation="MAIVVEERRRAKGWRWAGLVVLFAAGLEVCGGLSVSGAPSPVRL
                     SADSRLWLRGDSTLHPYSSTATKMDASGWYTGPSTGSGPEIAAALAAGALKDFSFSVS
                     VTDLSSGERLLDKKMRASLKAAAHPEIVFRMTDYQFPPWTGENQVVPVKARGLLSVAG
                     TEKPVELDGDLRVESRRLRISGRKELLMTDFGVKPPVMFFGALKTDNRVVVHYEIALE
                     LE"
     gene            4380..5672
                     /locus_tag="P5079_04770"
     CDS             4380..5672
                     /locus_tag="P5079_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY29335.1"
                     /translation="MKDIKKIFAAVVVVLGLSSRGVAMPVVERDNMDLNLGGRMQLFG
                     FGQQVHDPYRNDPRVYLFMKQARLQIWGHYEKYKYEVQLGFAGEEEVKAPSPGVSLNL
                     MDYSFDIPLADATFLKVGQYKVPYGRERLEDSGYLQFADRSIQNLALRVGRDVGFAVH
                     SYPGRLAWTAGVFTGGGRDVPERYLPQDLGVPMVVVRAGFNDGVDEDIYHSRQNDLDP
                     QRTKTAFFLNGLYMRDSLVGHSTVLNVKTSEKSLLVNSNWNPFVAQSPFDRGELVQGG
                     GDAAVRTPLGPFALSAEVEGNFGRYTNAYGSLQTTGGRAQLGLYRNPVELSIRYAFVI
                     PDSKFAYYDTATKKSYAITGHRAFQEVTPALTYYLKGRRSKIILDLPILVDVPVLTEK
                     NVGSYVVTEQPDQTSVLKAGSVDRQTVVEGRALFQVSF"
     gene            5702..6253
                     /locus_tag="P5079_04775"
     CDS             5702..6253
                     /locus_tag="P5079_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY29336.1"
                     /translation="MSGKVFRLALAGACFLVCAAAPRPGGVCLSVVQERHGNYRLEGA
                     FQVQSTPQVVWSVLTDYAGIPHFVSGLQSSRIVSRDGGRVRLEQTSSAKFLFLARAFT
                     VHLDVREHPPEKIDFADVSGKDFEVYEGSWFLEEKKGQLVVFYRLWAKPRFSVPAFLK
                     GWFFKRDVTRMLEEVKSEILRRD"
     gene            6617..7060
                     /locus_tag="P5079_04780"
     CDS             6617..7060
                     /locus_tag="P5079_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29337.1"
                     /translation="MKKTLIVMFLAVVFATTSLLAGHPGKQSCSYKAPEVVPAIGFEA
                     KMDNGRVVARWKKYNRPDFKYYKLVRSSTNANPVYPEDGYIFVGSQKDQTSYVDESVE
                     SGQWYYRVCVITTGGQRWVSPVVTLTVSDKSGGVSGGVPTSADFE"
     gene            complement(7484..7557)
                     /locus_tag="P5079_04785"
     tRNA            complement(7484..7557)
                     /locus_tag="P5079_04785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7521..7523),aa:Met,seq:cat)
     gene            7757..8347
                     /locus_tag="P5079_04790"
     CDS             7757..8347
                     /locus_tag="P5079_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29338.1"
                     /translation="MTTLRKAYGSYEIAKVCGVSPNTTWRWIRDGKLPSFKTGGGQRR
                     VWNEDLSLFLKDLGIPVPSKLMGAAAGATRVLIVDDEEDVRYTLKRMIRKFWPKAEIS
                     EAANGFDAGQKLLDLLPHVMLLDIALPGVDGFEICRTVRAHPKIKNVKILAISGYSVE
                     EWGKKVIEAGADAFLQKPIELETCRQAIQKWMEAPR"
     gene            8496..11027
                     /locus_tag="P5079_04795"
     CDS             8496..11027
                     /locus_tag="P5079_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY29339.1"
                     /translation="MTFFDFSHTRNNRGFGLVELMVTIALLSIGFLAAVSAFKGLSMG
                     IFKTKTKSLAVNLAQEKMEVLKDKSYYRLRVTSAPVAVPGTDPAVYSDAGNYAEETVY
                     VGGIPFFRYAVVEKVRKSASGGALEAVSWNEVDTGLKRISVGVVWTEADEKKQYVLVN
                     LRENPSRAAATVSMEGTVADAATMFPVSDAIVSVVENPSWRDRTDSSGRYSFNLSPGN
                     YTLRASSGPYFSDSRLGRSVDLSNTPLGTNFTLTKKANGTVTGTVWIRNHPVISQVGA
                     TLGGLDDEEFVELYNPSTYSITMYNSVFSTRKFVVKYVDIVGNETELNPIGGTTYNGV
                     GGQIIPANGFYLLASKNPIGGVQSNAYYNAPADRIPKEVAGGIVLCEYDGGVFISTWD
                     AVAWGRSGYSGPPKAREGNSYFTLTAGPGHGGLAPDETIERIAYATSTLVSMEPGTGV
                     HRALGNAYDDDVSADCWVHHDHNDWAGTLQNWNNPAFEFVTGETGTPANGAYIFADDG
                     MSNTAVAAPTGTPPRAQYTLTSVATGTWTLSASSGTLYISTGITVSASGSFQKPLHLS
                     STTDSGYVSGKVSDISGTPIPAVNVVPGGGTTDPSGFYRLALGEGLHNITANPGSLSA
                     SEYVEASVQVNVTRGQIASGVDITLSKGGGVRGRVTSDGTNALPDVPVSIFDSGTALE
                     VTNELTDSQGYFEIAVPTGSYYAVPIGEYGEVVTPAQSGVVTVTGGATVYTSTFTVGP
                     AVGYISGTVTAGGQPIPTGVLIVATTGTITGMLPDVDATLRSSGLVYYMATSGGTGRY
                     TLSVRNGTYNLYAWYTDASGNATPRSLTGVTVNPGQTTTADELTW"
     gene            11021..11551
                     /locus_tag="P5079_04800"
     CDS             11021..11551
                     /locus_tag="P5079_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY29340.1"
                     /translation="MVILGRLPLRAPRGLGGFTMAELMVVVAIMGIIFSIAPKVLMST
                     HRFFALSLARSEIQRDARTSLNMINRQLRQAKASTVTVSRHASDQPPYSKVEFQTVAG
                     STLTFWQNGHDLMMKKDSSNSRLLAKNIRNVAFAYTQTDNDAIMSVAVTFEKRIYEGK
                     SKSLQTAVEKVRIMND"
     gene            11569..12909
                     /locus_tag="P5079_04805"
     CDS             11569..12909
                     /locus_tag="P5079_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29341.1"
                     /translation="MKPILRKLWIRLSRDASGHSSPAAIAALLIVSISLPMVVKMVDN
                     ESKWAVKQKKTTTAFHLAEAGVDRAVWKLTEIEQNWLDAKEGTPIPGYDDDMEYTDVQ
                     GGEYKILISSGPTLGQVTIRALGRDSTQKEVRGIQAVYTRNVVDAALSVEGGLQYKPN
                     LHVHWGPVVNYTSISQSPGDYYPRKFSKGQIVGRDTVNDSVNTDGLEYWAFDTSLGTP
                     PQVDLDYYKEQAKNSVIPLEPSGGGRIEKNIGGNVLAVASPTGSGYFLASMNGNSGLR
                     FEKTGGSSFYDFRNSTSVIYIDNDTGAPIVTDLQNGGCFLRVEGLILAGDNHNMDCNA
                     QEGVLGATIPVHAQKEYKHSSAESYWNTNFSSVGEGNCCYALTKLAVHGFVYVGGNIA
                     NAGSNTEILGALDVKGNVNVNTLKVYYDEDIAEQVHLTGGTPRRLSWKEVNETW"
BASE COUNT         2564 a         3796 c         4197 g         2650 t
ORIGIN      
        1 gattggtgga ccacctacac ggtttctccg gtgggcaacg gcatcacgta tgccgtgttc
       61 aaggttttgg gggtcaacca cgggacgttg cgcctggccc cggccatcct gggatgcctc
      121 agcgtggcgt tcctgttctg gctggcgttt cgttacgccg gtccgcgtgc ggccctgttc
      181 gcgttgcttt tttcgggggt gaacgtgggg ctggtggcct acgcgcgatt ggccatgctg
      241 gagtcgttcc tgctcttttt ccagacatgg ctttatgtcc tttgtgcgcg ctggcttttt
      301 acccggcgcg gcgctttttg gatcggggtg ctgttcgtgg cggctttcgg cgcgaaggcc
      361 tccgcgatcc tcttcctcat tccgctggcg ctggtcctgt ttctcttttc cgccgccgac
      421 cggtcggtcg gcaggtcggg gtcggtgaag ggcttcgtcc tcggcggcgg ggccgcctcg
      481 tttctctacc ttctgttcgc cgccgtgaac tggccgtttt tctaccactt gaacatgggt
      541 ttttccgccc acaagaccat cctggaccgc agcagcggcc tgctgaaagt gttggaactg
      601 cccaccggca atggagaact tctccttacg ctgatgccgc cgttgttgtg ggcggcgtgg
      661 ctggggatgg ggcgtctgtg gaacggggag gggttgtcgg ccctctggga atccgccggc
      721 cccccgcgtc aacggatacg cggggcgttg gaacggatcg cgcgactgga cccgttcccg
      781 aggtgggcgc tgttctgcgt gctcgttttt atcggggatc tcctggttca tttgtttctg
      841 cggatggatg tctcggcgcg gcgtcttctg catatgctgc cgccgatgat cttgacggcg
      901 agtttggggc tggaccgatg gttgtcgggg gaggggcgtc cctcgcgcgg cggatttttc
      961 ggcgtgtttt cggctttcct cgtcggctgg gtggccgtca aggccacggt gttctgtttt
     1021 ttcggtgtgt ccggctggtc gaacccggcc acattcctgt tcacccggcc gttcccggtt
     1081 ttccgcacgc cgggcgatca gtccctcttc ctcgggcggg gatggttcga gatggggtat
     1141 gcgcttgttt ttgcgggggc gtggctcctg tggaaatatc ggcgggcccg gcccggaagc
     1201 gctccggagc ggacggaagg gcttcccgga ggccgctgga aaccggtcgt cgcctggacg
     1261 gtcgcggtgg tgtatctcgg ccatcagttg tcgatggtgt tgccgccttt gctgaagccg
     1321 acctatctct atcgggacgc ttcccgcgcc attgaacgtt tcagcgagag gccgggggcc
     1381 gtggtgctcg ggaacatggc cgccgggctt ccctgggagg gcagctatta tccgctctat
     1441 tacatgatga gcgccggcat cggttataag aaaaaaacca tggaggacat gggaaggatc
     1501 gtcgacaaat atcggcccac tctcatggtg gtgttcgatc ccgacggcaa ggtgggggac
     1561 ggcgccctct acgactggtc ttcgccggac gccgtcgacg aggagacccg gcgcaccatg
     1621 gaaaagcata gcccccggat cttcgatttc cgggaaatcg tttaccgttg gaaagggctg
     1681 gaaggcctgt atccccgggg gcagtggctg cttttgaaaa gaaaaatttc ggagcccgcg
     1741 tcccgcctga aaaaatgacg cccctgtacg acgcccctcg gccggacatc ttgttcgcca
     1801 tgtcgcccgc gcacgcccgc gacatgtaca tgcccatcta tttcctgtac ctggcggggt
     1861 atctcgagaa aaaagggtat cgcacggaga tcgtcgatcc tcatcggcgc ggcgcggagg
     1921 ataacatccg gatcatcttg gaccgggtgg ccgcgcggcg gccgaggtat gtgggccttt
     1981 cctcgttcgc aaccgactac ggctgcgccg ttgagctggc ccgtcggatc aaggagattt
     2041 ccgacgcgaa ggtcgtcgtc ggcaatgcgc agccctcctt gacgcccgag gattatttgt
     2101 tcgacggaag cccgttcgac atcgtggtcc gcggcgaggg cgagttgacg ctggaagaga
     2161 tcctctcccg cgggccggct gggggcgatc tgtccggcgt tcgggggatc gcctaccggg
     2221 aaaacgggcg cctggtgctt acggaaaagc gggagctcat ggatctggcg ttgtgcgcca
     2281 tgcccgcgta tcacaaggtc gacatgcgct ggtatcgccg ggccacaaag cacatcatcc
     2341 gccgtctttt caccgtcggg gcggtcgtct acacgggccg cggatgccct ttccgctgtt
     2401 ctttctgcgc ggccaactcg gtgtggcaga ccaatcggat cacgacggcg catccgatgg
     2461 tgagaaagcg gccgttgccc catgtgatgg ccgaattgcg cctgctgcag gacacctacg
     2521 ggttcgattt tttctacata ttggacgaca ccttcggcat tcacgagaag gacatcaccg
     2581 atttttgcga ggcctaccgg gccagcgggt tgcgaatgtt gtgggccgcc gagaccaggg
     2641 tcaactgcct gaaaaacgac gccatcttca aaacgctcaa gtcggccgga tgcctccagc
     2701 tggatttcgg cgtggagtcg gggtcgcaga agctgttgaa cgccatttgc aagggcatca
     2761 acgtcccaca ggtcgtggag gcgttcgacc tctgccggcg gaacggcata aggacgttcg
     2821 ccaatatcct gctgaacctg cccggagagg acgagaacga tttggttctg acggagcaac
     2881 tgttggaaaa aataaggccc gactatatct ccatcggcgt cacgcagccg tatcccggaa
     2941 ccgccatcag ccggggattg tcccggccgc tgacacggga cgattatgcg gagctgagcc
     3001 ggttgatccc ttcggagaaa ttcaggctca gcaaacacaa ccgggatctt tacaaactgg
     3061 tgtccgcctg gcttttgaaa tatcgggcca ttgcgccctt tgaccggagc gtgctccggg
     3121 cggacgcttc ctattggaag gcgttgctcg cttcgaacaa caaactcaac tacgcctatt
     3181 tcatcttgag ggatctggtc acctacccgg tggtgttcgc gatattcctg atccggcgga
     3241 tcgggctcct ctggcgcccg agccatgtcc ctccgtgggc cgaccccatg aaagaaaaag
     3301 accccggcca atagtttctt cgaattttgg ggggataaaa tccaaccctc tccccgaggg
     3361 cgttcccacc gcctcgtctt taccgaaaaa gccacgaaaa cttccgtggc ttttttgttt
     3421 cttcaggcgc tccccgcctc ctcgccctcc tgagaacagg aaacaaaagc gtctttccgg
     3481 tgttatagaa aaaagacggc ggcctccgtc cggaagagtc gtcgggaagg cgtctctctg
     3541 gaaagggggg ggtcgtttcc ttggggcaga ggcacatacg ccgtcggtgg tttctatgac
     3601 cgccgtcatg gatgaaaaca ggggggtcgt tctatactga gagagacggg attctggtga
     3661 agggaggggt atggctattg tcgttgagga gcggcgtcgg gcgaaggggt ggcgctgggc
     3721 gggtctcgtg gtcctttttg ccgccggcct ggaggtgtgc ggagggctct ccgtctcggg
     3781 cgctccgtcg ccggtgcggc tctccgccga cagtcgtttg tggttgcggg gggattcgac
     3841 ccttcacccc tattccagca ccgccaccaa aatggacgct tccggctggt acaccggccc
     3901 gtccacgggc tccggtcccg aaatcgccgc cgctctggcg gccggcgccc tcaaggattt
     3961 cagtttttcc gtgtcggtga cggacctttc ctccggggaa cggctgctcg acaaaaagat
     4021 gagggcttct ctcaaagccg ccgcgcatcc cgagatcgtt tttcgtatga cggactacca
     4081 attccctccg tggacgggag aaaatcaggt tgtgccggtg aaggctcgcg gactcctctc
     4141 cgtggccggg acggaaaagc cggtcgaact cgacggcgac ctgcgcgtgg agtccaggcg
     4201 cctccggatc agcggccgga aagaactgct catgacggat ttcggcgtca aaccgcccgt
     4261 catgtttttc ggcgcgctga agacggacaa cagggtggtc gtccattacg agattgcgct
     4321 ggagcttgaa taggggccgg ccggcggaac cggatgaaaa aatgaaaagg agaatgatta
     4381 tgaaagacat caaaaaaatc ttcgccgcgg tggtcgtcgt tttaggactt tcgtcgcgcg
     4441 gtgtcgccat gccggtcgtc gaacgggaca acatggacct caacctgggc ggaaggatgc
     4501 agctcttcgg gttcggccag caggtccacg atccctaccg caacgatccc cgcgtctacc
     4561 tgttcatgaa gcaggcccgc ctccaaatct gggggcatta cgaaaaatat aaatacgagg
     4621 tgcagctggg tttcgccggg gaagaggaag tgaaagcgcc cagcccgggg gtgtcgctga
     4681 acctgatgga ttactccttc gacatccctc tggccgacgc cacttttctg aaggtcggcc
     4741 agtacaaggt tccctacggc cgggagcggc tggaggactc ggggtacctg cagttcgccg
     4801 accggtccat ccaaaacctc gccctgcgcg tcggacgcga cgtggggttt gcggttcatt
     4861 cctatccggg ccgcttggcc tggacggccg gcgttttcac cggcggcggg cgggacgtgc
     4921 ccgaaagata cctgccgcag gacctcggcg tgcccatggt ggtggtgcga gcggggttca
     4981 acgacggggt ggacgaggac atctatcact cgcgtcagaa cgaccttgac ccgcagcgca
     5041 ccaaaacggc cttcttcctc aacggtctct atatgcggga ttccctcgtc ggccattcca
     5101 ccgtgctgaa cgtgaagacc tcggaaaaat cgctcctcgt caacagcaac tggaacccgt
     5161 tcgtcgccca gtcgcctttt gacagggggg aactggttca gggcggcggc gatgcggccg
     5221 tgcggacgcc gctgggaccg tttgccctgt cggcggaggt cgagggaaat ttcggccggt
     5281 acaccaacgc ctacggcagt cttcaaacca ccggcggccg ggcgcaactc gggctttacc
     5341 gcaaccctgt tgaactttcc atccggtacg ccttcgtcat cccggacagc aaattcgcct
     5401 actacgatac cgccaccaaa aaatcctacg ccatcaccgg ccaccgggcc tttcaggagg
     5461 tcacgccggc gctcacctac tatctgaaag gacgccgttc caaaatcatt ttggacctgc
     5521 ccatcttggt ggacgtcccc gtgctgacgg agaaaaacgt ggggtcctat gtggtcacgg
     5581 aacagccgga ccagacgtcg gtgctgaagg ccggatccgt ggaccgacag acggtggtgg
     5641 aaggtcgcgc tctttttcaa gtgagctttt gacgacggga aaagaggggg gtgagggggg
     5701 gatgtccgga aaagtatttc gcctcgcttt ggcgggggcg tgttttttag tttgtgcggc
     5761 cgcgcctcgg cccggcgggg tatgcctgtc cgtcgttcag gagcgccacg ggaactaccg
     5821 gttggaaggc gcgtttcagg tgcagtcgac tccgcaggtg gtttggagcg tgctcacgga
     5881 ttacgccggg atccctcatt tcgtgtccgg ccttcaatcc agccggatcg tttcgcggga
     5941 cggcggccgc gtgcggttgg agcagacctc ttccgccaag ttcctgtttc tcgcccgtgc
     6001 gttcaccgtg catctcgacg tccgcgaaca ccccccggaa aaaatcgatt ttgcggacgt
     6061 ttccgggaaa gattttgagg tatacgaggg gtcctggttc ttggaagaaa agaaaggaca
     6121 gttggtcgtc ttctatcggc tgtgggcgaa gccgcgtttt tctgttccgg cttttttgaa
     6181 gggatggttc tttaagaggg acgtgacgcg gatgctggag gaagttaaat ccgagatttt
     6241 gagacgggac tgagggtgtc ggacaggaca tccccgtaaa tgtgacgccg cggccgacac
     6301 cccgggcgat tttaatggcg aggtttctcc cggcatgcaa caaagaaaaa gagtttgtcc
     6361 cattctgtgg ggcaagcttt ttttgtttgg agcgacggga gacgaagccg cggggccgac
     6421 agggcgggac aggaacgacg gatcgggttc atcggacggc cgggccatgc cctcctccgt
     6481 ccggcggggg aggccgatgg gcggcccgcc ccgctcatcg ccggaccgag acgaggcggg
     6541 tttgtttccc ggtcccccga cgggatcgtt tgtgaactgg tataatcggt ctatcttgat
     6601 tcaaatcgga ggtcttatga aaaaaacgct gatcgtgatg tttttggcgg tggttttcgc
     6661 gacgacatcc ctcctggcgg gacatccagg gaaacaatcg tgttcctaca aggcgcccga
     6721 ggtggttccg gccatcgggt tcgaggcgaa aatggacaac ggccgggtgg tcgctcgttg
     6781 gaagaagtat aaccgtccgg attttaaata ttacaagctc gttcgatcca gcaccaacgc
     6841 caaccccgtc tatccggaag acggctatat atttgtcggt tcccagaaag accaaacctc
     6901 gtatgtggat gaatccgtgg agtccggcca gtggtattac cgtgtctgcg tcattaccac
     6961 cgggggacag cgctgggtga gcccggtggt taccctaacg gtcagcgata aaagcggcgg
     7021 ggtgtccggg ggagtgccga cgtcggcgga tttcgagtag cgaagaaaaa ttcccggaag
     7081 gccgccggcg gaaaaggcgt tgggagctcg ttaacatcgt tgcccgggcg cttgcgcgcc
     7141 catcccgacg gttgccgccg gtcggcccct gcaagtctcg atcagagata ggaacctggc
     7201 gtaaatcaaa agagccagca agcccatcga gggctgtctg gcttttttgc tggagggggg
     7261 ggcgccctcg tcggctcgct ccgaggatca gcggcagtcc ctccaaaacg tttcgctgaa
     7321 actgaaggac ctgccgcgcc gaccggcgga cggaagactc cgccgtgatt tgaatcgacg
     7381 cacaatgctc tcgcgctcca ttcgggctcg agggcgcagg tttataaatc gaccaaaaaa
     7441 cagaaaagcc gttagcaccc ttagggtgcc gacggctttt ctgttgcggg ggtaggattt
     7501 gaacctacga cctcgaggtt atgagcctcg cgagctacca ggctgctcca ccccgcaaaa
     7561 ggattatagc ccaaatccag ctccaaaagc aaacgtatat aaggagattt attaaagggg
     7621 aatcgacagg tcacaggggg gggcactcac cgcgcttcga gggagcggaa cgggtgaaaa
     7681 atacctcttg acaaaagggg gaacggaata tatgctatac atgtaataca tgtcataaat
     7741 aatatacatt caatatatga ctacgctaag aaaagcctat ggttcctatg aaatcgccaa
     7801 agtgtgcggc gtttcgccca acaccacctg gcggtggatt cgggacggca agctcccttc
     7861 ctttaaaacc gggggggggc agcgtcgtgt ctggaacgag gatttgagct tatttctaaa
     7921 ggatttgggc atccccgtcc cgtcgaaatt aatgggcgcg gccgcggggg ccacccgcgt
     7981 tttgatcgtc gacgacgaag aggatgtgcg gtacaccctc aagaggatga tccgaaaatt
     8041 ttggccgaag gcggagatca gcgaggcggc caatggtttt gatgcggggc agaaactgct
     8101 cgatcttctt ccccatgtga tgttgttgga catcgccctc cccggggtcg acggatttga
     8161 gatttgccgc acggtccgag cccaccccaa gataaagaac gtcaaaatac tcgccatctc
     8221 cggttacagc gtggaagagt gggggaaaaa ggtcattgag gcgggggcgg acgcttttct
     8281 gcaaaaaccc atcgagttgg agacctgtcg gcaagccatc caaaaatgga tggaagcgcc
     8341 ccgctgaacg cggtctcgcg ttccatcaac agcacgatcg cccgcggttt ggagacggac
     8401 accttggtca aggagagggt cgcccctatg aggtgagcag gttgttcgcg gcgccctcgg
     8461 gggcttggag gggaatccca catgggagga aaccggtgac atttttcgat ttttcccata
     8521 cacggaataa caggggattc ggcttggtgg agctgatggt caccatcgcc ctgttgtcca
     8581 ttgggttttt ggcggcggtg agcgcgttca agggcctgtc catggggatc ttcaagacga
     8641 agacgaaatc cctggccgtc aatctggccc aggaaaaaat ggaggtcctc aaggataaat
     8701 cgtattaccg gctgcgggtg accagtgcgc ccgtggccgt gccggggacg gatcccgccg
     8761 tctattccga tgccggcaac tacgcggagg aaaccgtgta tgtggggggc atcccttttt
     8821 tccgttacgc cgtggtggag aaggtgcgca agagcgcgtc gggcggcgcg ctggaggcgg
     8881 tgtcgtggaa cgaggtggac accggcctga aacggatctc ggtgggcgtg gtgtggacgg
     8941 aagcggacga gaaaaaacag tacgtcctcg tcaacctgag ggaaaacccc agccgggccg
     9001 ccgccaccgt gtcgatggaa ggcaccgtcg ccgacgcggc cacgatgttc cccgtttcgg
     9061 acgccatcgt gtcggtggtg gaaaacccgt cctggcggga caggacggat tcctccggcc
     9121 gatattcgtt caatctttcc cccgggaact acaccctgcg ggcctcctcc gggccgtatt
     9181 tctccgacag ccggctcggg cgctccgtcg acctctccaa cacccccctc ggaacgaatt
     9241 tcaccctgac caaaaaagcc aacggcaccg tcacaggaac cgtttggatc cggaatcatc
     9301 cggtcatttc ccaggtgggc gccacgctgg gcgggttgga cgacgaggag ttcgtggagc
     9361 tctacaaccc ctcgacctat tcgatcacga tgtataactc cgttttctcc acgcgcaaat
     9421 tcgtcgtcaa atacgtcgat atcgtcggga atgaaaccga actgaacccc atcgggggga
     9481 ccacctacaa cggcgtgggc gggcagatca ttccggcgaa cggtttctat ctcctcgcca
     9541 gcaagaatcc catcggaggc gtccagtcca acgcctatta caatgctccg gcggatcgga
     9601 ttccgaagga ggtcgccggg gggatcgtcc tgtgcgaata cgacgggggc gtcttcatca
     9661 gcacgtggga cgcggtggcc tgggggcgtt ctgggtattc gggcccgccc aaggcgcgag
     9721 aagggaacag ctattttacg ttgaccgccg gaccgggaca cggcggcctg gcgcccgatg
     9781 aaaccatcga acgcatcgcc tacgccacgt cgacgctggt ctcgatggaa ccgggcacgg
     9841 gcgtccaccg cgccctcggc aacgcctacg acgacgacgt ttcggcggat tgctgggtgc
     9901 accacgacca caacgactgg gcgggcactc tgcagaactg gaacaatccc gctttcgaat
     9961 tcgtcacggg agaaaccggg acccccgcca acggcgccta cattttcgcg gacgacggca
    10021 tgtccaacac cgcggtggct gctccgacgg gcacgcctcc ccgcgcccaa tacacgttga
    10081 cgtccgtcgc cacgggcacc tggacccttt ccgcctcctc cggcaccctc tacataagca
    10141 ccggcatcac ggtcagcgcg tcgggcagtt tccagaagcc cctgcacctg tccagcacca
    10201 ccgattcggg atacgtcagc ggaaaagtga gcgacatctc cgggacgccc atcccggcgg
    10261 tgaacgtggt gccgggcggg gggacgaccg accccagcgg gttctaccgg ttggcgctgg
    10321 gggaaggcct ccacaacatc acggccaacc cgggcagttt gagtgcttct gaatacgtgg
    10381 aggccagcgt gcaggtgaac gttacccggg gacagatcgc gtcgggggtg gacatcaccc
    10441 tttctaaggg cgggggggtg cgggggcgcg tcacgtcgga cgggaccaac gcgctgccgg
    10501 acgtccccgt gagcatcttc gattcgggaa ccgccctgga ggtgaccaac gaactgaccg
    10561 acagtcaggg ctattttgag atagccgtgc ccaccgggag ctattacgcc gtgcccatcg
    10621 gcgaatatgg ggaagtggtg acgcccgccc agagcggcgt ggtcacggtg acgggagggg
    10681 cgacggtata cacctccacg ttcacggtcg ggcccgcggt gggttacatc agcggcaccg
    10741 tgacggcggg agggcagccc atccccacag gggtcttgat cgtggccacc acggggacca
    10801 tcacggggat gctgccggac gtcgacgcca cgctgcggtc cagcggactt gtctattaca
    10861 tggccacctc gggcgggacg gggagataca ccctgagcgt gcggaacggg acctacaacc
    10921 tctatgcctg gtatacggat gcctccggca acgccacgcc tcggtcgttg acgggggtca
    10981 ccgtcaaccc gggccaaacc accacggcgg acgagttgac atggtgatct tggggcggct
    11041 ccctctccgc gcaccccggg gcctgggcgg tttcaccatg gccgaactga tggtggtggt
    11101 cgccattatg gggattattt tcagcattgc gcccaaggtg ttgatgagca cgcaccggtt
    11161 cttcgcgttg agtttggccc gttccgagat ccagagggac gcccgcacgt ccttgaacat
    11221 gatcaaccgg cagcttcgac aggccaaggc ctccacggtg acggtgagcc ggcacgcctc
    11281 cgaccagccg ccgtattcca aggtcgagtt tcagaccgtc gccgggtcta cgctgacgtt
    11341 ttggcagaac ggccacgact tgatgatgaa aaaggacagc tccaactcca ggctgctggc
    11401 caagaacatc cggaatgtgg cgttcgccta cactcagacg gacaacgacg ccatcatgtc
    11461 ggtggccgtg acgtttgaaa agcggattta tgaaggcaaa tccaagtcgc tccagacagc
    11521 tgtggaaaaa gtccggatca tgaacgactg aggcggaagg agacgaccat gaaaccgata
    11581 cttcgaaaac tttggattcg gttgtcgaga gacgcgtccg ggcacagctc gcccgccgcc
    11641 atcgcggccc tgctgatcgt ttccatctcg ctcccgatgg tcgtcaagat ggtggacaac
    11701 gaatcaaaat gggcggtcaa gcagaagaag acgacgacgg cgttccacct cgcggaggcg
    11761 ggggtggacc gcgccgtgtg gaagctgacc gagatcgaac agaactggct cgacgccaag
    11821 gaggggaccc cgatccccgg ctacgacgac gatatggaat acaccgatgt ccagggaggg
    11881 gaatataaga tcctcatttc ctccggccct acgctgggcc aggtgaccat ccgggcgttg
    11941 ggacgggact cgacccaaaa ggaggtgcgg gggattcagg ccgtgtatac ccggaacgtg
    12001 gtcgatgccg ctttgagcgt ggagggcgga ctccagtata aacccaatct ccacgtgcat
    12061 tggggaccgg tcgtcaacta cacctccatc tcccagtcgc cgggcgacta ttacccgcgg
    12121 aaattctcca agggccagat cgtcggacgg gacacggtga acgattccgt gaacaccgac
    12181 ggcctggagt attgggcctt cgataccagc ctgggcaccc cgccccaggt ggatttggat
    12241 tattacaagg agcaggccaa gaattcagtg atcccgttgg agccgtccgg cggtggccgc
    12301 atcgaaaaga acatcggcgg gaacgtcctg gcggtggcct ccccgacggg gtccggatac
    12361 tttttggcgt ccatgaacgg caattcgggg ctccgtttcg aaaagacggg cgggagcagt
    12421 ttttatgatt tccggaattc cacgtcggtc atctacatcg acaacgacac tggggcgccc
    12481 atcgtcacgg accttcaaaa cggcggttgc ttcttgaggg tggaggggct gatcttggcg
    12541 ggggataacc acaatatgga ctgcaacgcc caggagggcg ttctgggggc caccatcccg
    12601 gtccacgcgc aaaaggaata taaacattcc agcgcggagt cgtattggaa caccaacttt
    12661 tcatccgtcg gcgaaggcaa ctgctgttat gccctgacca agctggcggt gcacgggttc
    12721 gtctacgtgg gaggcaacat cgccaacgcg ggaagtaaca cggagatttt gggcgcgttg
    12781 gacgtgaagg gcaacgtgaa cgtcaatacg ctcaaggtct attacgacga agacatcgcc
    12841 gaacaggtcc acctgaccgg cggaacgccc cggcggttgt cctggaaaga ggtcaacgag
    12901 acctggtagc cccgtttttt tgtccgaccg gaatcaaaga tcccccacgc gcggccgatc
    12961 ggcccgcccc cgcccgtccc gtcgtcacat aaatccgaaa agtgagagcg agccccctgt
    13021 tgagggcggt ttgtgggggt ttcagaacca ccggggggag ccgaaggtgg gcaggaacgg
    13081 ccgctgacgc gccgcttttt tggagtgacg tcaaacaggc gaaatcgtaa acccgaggtg
    13141 aaacttctcc gccggtcggc atttacggtt ataatgaggt ggcggaagac gaaaagagct
    13201 tttttct
//
LOCUS       DAOTFD010000053        13187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3275_length_13187_cov_3.771474, whole genome shotgun sequence.
ACCESSION   DAOTFD010000053 DAOTFD010000000
VERSION     DAOTFD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3275_length_13187_cov_3.771474"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="P5079_04810"
     CDS             complement(<1..431)
                     /locus_tag="P5079_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRY29342.1"
                     /translation="MGQILTVAWVYGLLALDHIAVGQFMLSRPLVVGPLLGWCLGNAP
                     LGLLAGASVEMFWVHPIPVGHWPIDPAAVAALSITWCQLSSHPGRGTLTLCLLLAIPC
                     GIVVRHLDIWFRRQNSRFIPWVSERLERGNEGALGGAVALS"
     gene            complement(452..967)
                     /locus_tag="P5079_04815"
     CDS             complement(452..967)
                     /locus_tag="P5079_04815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRY29343.1"
                     /translation="MPLVLVRIDDRLIHGQVVEGWLRVIQASLIVVVSDEVAEDPFQV
                     SLMGLAVPPDVRVVALKVSDAADFLKSDAVKDDRVMVLMAGVRAARRLVESGVEMATL
                     NLGGLHEAAGRVALTPFLCLNADDRADIEFLLKKGVYVEVRALPNDEKRNVREFLNRS
                     SAPEAKDIRPQ"
     gene            complement(967..1392)
                     /locus_tag="P5079_04820"
     CDS             complement(967..1392)
                     /locus_tag="P5079_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY29344.1"
                     /translation="MIGVLIVTHGRIGEELLRTAEGIVGKQVAVRILGLDATEGPDAF
                     CKKIRVVLEEMKNPAGVLVLADMMGGTPCNSALRQCQDPQWNFEIVTGVNLPMLVSVL
                     TKRHYMPLDLLAQKITDDGPRTIQRPVQKLRTAGRRENA"
     gene            complement(1456..2463)
                     /locus_tag="P5079_04825"
     CDS             complement(1456..2463)
                     /locus_tag="P5079_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRY29345.1"
                     /translation="MESPRIVVLGGGTGLSTLLRGLKQFTSDLTAVVTVTDEGGSSGR
                     LRRHWGVLPPGDLRNCLVALAQDDTLVARLFQYRFPHSAVGGSKQDSLAGHSLGNLLL
                     TGLCDVAGGIDRAMAAAGQVLAIRGRVLPVSLQHLRLHARLADGRRVLGEPRISRSRT
                     RIQHVYLTPSRAQAAPGVLKSLRDADAIVIGPGSLYTSVIPPLLVDGVAEAVARSRAR
                     KIYVCNVMSQPGETDGYSAVDHLRAVLEHCGSGIRERVVDAMLLNNRAFPEPILRHYA
                     AFDSYPVDPPRQDHFGRVRIIGADLRPSFRGRDAVPALARHSSGRLAGAVIDLLRNRK
                     I"
     gene            complement(2489..3388)
                     /gene="rapZ"
                     /locus_tag="P5079_04830"
     CDS             complement(2489..3388)
                     /gene="rapZ"
                     /locus_tag="P5079_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRY29346.1"
                     /translation="MKKSRGSRPAVSGGGYDCVIVTGLSGAGKSVALRALEDLGYFCI
                     DNLPLPLLPSLIRFYRGWGQRLRRIALGVDVRTGLPAREFRRSLENLRREGFQLRVLF
                     LDSDNTTLLRRFSETRRRHPLGGTILGGLRRERRLLRDIKMLADWQIDTSRLAPSEVK
                     EIVVQTLGLRHPQGMTVQVMSFGYKYGIPLDADLVWDVRFLPNPNYVPQLRARTGKDN
                     PVSRYVLRDPTTQKFMAHLRQMMEFLLERFVLEGKSYLTVSVGCTGGRHRSVAVAEAL
                     ADFIRTHLKMPVRVQHRDIARRG"
     gene            complement(3470..4444)
                     /gene="hprK"
                     /locus_tag="P5079_04835"
     CDS             complement(3470..4444)
                     /gene="hprK"
                     /locus_tag="P5079_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRY29347.1"
                     /translation="MSINPASGTPAPLLVEEFWKEQASSLRLSLVSGQSGLNRQIRVT
                     DINRPGLTLAGFFEYYRGERIQVFGQGECAFLATLASAQRMDILDRILSSGDLPCLIL
                     THGREPIPEMQEQCDRYKVPLFQSQWETARLIGELTAYLEERLAPYTSVHGTLVNVYG
                     MGVLILGSSGIGKSECALELLKRGHMLVADDRVEIRQKPGGILVGSGSPLVQHHMEVR
                     GLGIIDVWKIFGVGAVIKRTIIELVLYLEMWDQNKNYDRMGLEEQHSVLLGVRVPEIR
                     VPIHPGRNLAALIEVAALNQRLKQEGIHSARELNERLIQKMSGHSDRL"
     gene            complement(4434..4892)
                     /locus_tag="P5079_04840"
     CDS             complement(4434..4892)
                     /locus_tag="P5079_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY29348.1"
                     /translation="MKLADILAPEVITVDLKAQGKQEVLEELCGLLSAANKLPDPKQM
                     VSVLMEREALGSTGIGQGVAIPHGKVNSVTQQVAALGLSRRGVDFEALDGESVYIIFL
                     LVAPLDAAGNHLKALAKISRLLKDKYFRQSLRDAKTPEDVLKIIREEDEY"
     gene            complement(4915..5451)
                     /gene="raiA"
                     /locus_tag="P5079_04845"
     CDS             complement(4915..5451)
                     /gene="raiA"
                     /locus_tag="P5079_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRY29349.1"
                     /translation="MQVNITARHLDLTPSLADYVQKRLERVGRHFESVIRAQVVLEVE
                     KHRHIAEIVVHANGHHDFRVKGESADLYAAIDLVAEKLHKHMAREKDRRVRGRRQSKR
                     SLVGLPIPLIPSDGDDSFALPLTQVRRFTPRPMTVEEAVEEMEQKNYMFYVFMNDDIV
                     SVVYKRKDKTYGLLESNL"
     gene            complement(5602..6327)
                     /gene="lptB"
                     /locus_tag="P5079_04850"
     CDS             complement(5602..6327)
                     /gene="lptB"
                     /locus_tag="P5079_04850"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRY29350.1"
                     /translation="MSMPLHADALKKRYGVRTVVDGVSLEVNPGEIVGLLGPNGAGKT
                     TSFYMMVGLVRPDGGAITLDGQDVSDLPMYRRARTGLGYLCQEPSIFRRMTVEDNLMA
                     ILENLPLDPSEQKNKCEKLLQELGLDRLRKQMAYTLSGGEKRRTEIARALTTDPRYLL
                     LDEPFVGIDPIAVNDIQEVLRALKKRGLGLLLTDHNVQAMLEIVDRAYIIYSGRILVQ
                     GNAQDLLNSPEARRVYLGDRFRL"
     gene            complement(6305..7129)
                     /locus_tag="P5079_04855"
     CDS             complement(6305..7129)
                     /locus_tag="P5079_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29351.1"
                     /translation="MNRISPFALAVSVVLGLAIAGLTEEPKPPKTVITGDRMNLLRKG
                     AAVEFVGGVTLVRGNDFMKADRMVSEEKKGLTEAWGRVYLRRDDPLGGVRWESWAEEG
                     TYSSQISSGTLWGRGKPVKVHRSPLSASTETQRMEMEALRFTFYDRVVESATQSTSAW
                     TCVEAEGLVYVWMKESTPTVRDTQIWAHDAFYDGSMENVRFWGGYDSFRNDSRAADPK
                     PVQPRARQTEGEEIRDLSGDEMTYFLNDKRLVVERNVRATILTPDSPLEDVHAPAR"
     gene            complement(7126..7707)
                     /gene="lptC"
                     /locus_tag="P5079_04860"
     CDS             complement(7126..7707)
                     /gene="lptC"
                     /locus_tag="P5079_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRY29352.1"
                     /translation="MKPFRFLVFVAAIVLGGGFLASCRSSHPAGRVEKAAAVSPDQVI
                     RNFSIDSFAGKEKDWHLESPRADIFESENRIRIQKPSIRFFENSEPSSSLEAGAGEIQ
                     TSGARDLWAWDQVVMTSTDGARLTSEWLHYISEKERIVSTAPVTIVRPGTTIQGVGWE
                     ATPDLSEVLVRQQRVEIVGETPLAGGKGGGKDK"
     gene            complement(7704..8288)
                     /locus_tag="P5079_04865"
     CDS             complement(7704..8288)
                     /locus_tag="P5079_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRY29353.1"
                     /translation="MRTRSSLRRRAAAVRLLAMDVDGVLTDGSIVLLKSGEEVKVWNV
                     KDRIAFSMLEKSGRRFYVAWITGRASAQVKARAEELGVDALYQSCLDKRAALQDILRR
                     FRLTARQALYIGDDLIDIAALKGAGLAVCPSDAVAEVKSVCSLVTRAPGGRGVFREAV
                     DFVLRSQGLLRKVVAEFERPQSRLFKDAQGKKRR"
     gene            complement(8293..9273)
                     /locus_tag="P5079_04870"
     CDS             complement(8293..9273)
                     /locus_tag="P5079_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRY29354.1"
                     /translation="MKIRELKDWRRRVQRTLRLEAQAVTHQLRHIDEHFLLAARCLSR
                     CRGRVVVMGIGKSGLVGRKLAATLASVGTPAFFVHPTEALHGDSGMFSTGDIVLVLSY
                     SGETEELKRVLSVVHQRKLPLIALTGRPRSMLGRSVDWVLNVAVEKEACPFNITPTAS
                     TTAMLAMGDALAMLVMELKGFDKEDFARLHPGGTLGKKLTLRVADIMHKGSENPLVAD
                     SQKVREALWVMTKTRMGAASVIDRRGRLAGVFTDGDLRRGLQKDDRLLDRPLRQVMTR
                     SPRTVAPGDLAADVALLFRRFGLDNLPVVDRTGRPVGILDEKDLLEEGLG"
     gene            complement(9302..9685)
                     /locus_tag="P5079_04875"
     CDS             complement(9302..9685)
                     /locus_tag="P5079_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29355.1"
                     /translation="MKVCPQCSAENEPDHNFCERCGDSFLERWEPFQRPRYEALARFS
                     NGARYLARLSAGAGGLCALGVLAYYWNKSLLVAVFFAGAAALAGYLLLVAFQAVAEFI
                     RLFVDIEDHLRSSRDGHAPKPPAPH"
     gene            complement(9723..10385)
                     /locus_tag="P5079_04880"
     CDS             complement(9723..10385)
                     /locus_tag="P5079_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY29356.1"
                     /translation="MSERTGFLFPCPQCGASLHDRARYCGHCGVSIALWDFTWSLRRS
                     IGGAVAGGVGWVLGSTFLRLTLPGADLSLPHPLFLTVAGLFLGMAGGLMERSSSRVWR
                     GLFTGGAAGLSFGTAARFLARGASPDALFVLHVAVWALFGMAIGFSVGRLKGWETVGG
                     FLWAVWRGRWRGISRWPWPCSSLNRAGPRPAEWSSFPAPSWARSSGAGWPSAAGSADG
                     GG"
     gene            complement(10394..10714)
                     /locus_tag="P5079_04885"
     CDS             complement(10394..10714)
                     /locus_tag="P5079_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY29357.1"
                     /translation="MKCPKCGIENKNNAKFCNDCGAPLSSSSAKGAKIAPAAPPRDFA
                     GRSDLSTDNFWKPDWRWHAKALLVIYVSLTVIYFVLSLVLSKVPEPYRMRDIPKEMTP
                     WLKQ"
     gene            complement(10738..11577)
                     /gene="kdsA"
                     /locus_tag="P5079_04890"
     CDS             complement(10738..11577)
                     /gene="kdsA"
                     /locus_tag="P5079_04890"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRY29358.1"
                     /translation="MSQQREIKIGSCRLANDRPLAVVAGPCIIESEKVTLRIAAELAA
                     VRRRLNVPLIFKCSYDKANRSSVDSYRGPGLARGLKVLRRVKSEFGLPLLVDVHSVGE
                     VPRVAEVADVLQVPAFLCRQTDLLLAAARTGLPVNVKKGQFLSPWDARLILKKAESIG
                     HRDLFLTERGVSFGYNNLVVDMRSLEVLKSFGRPVIFDATHSVQLPGGRGDSSGGQRE
                     FIAPLARAAVAVGVAAVFFEVHPRPEHALSDGPNAVPLRELEPLLRRLLAIDRVVKRF
                     VNN"
     gene            complement(11582..13186)
                     /locus_tag="P5079_04895"
     CDS             complement(11582..13186)
                     /locus_tag="P5079_04895"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRY29359.1"
                     /translation="MTRYIFVTGGVVSSLGKGISAASLGRLLKSRGLNVTMIKLDPYI
                     NVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFLDMDMARENSCTSGQIYDTIIQQE
                     RRGEFLGNTVQVIPHVTNEIKNRLKRVARGRDVLIAEIGGTVGDIESLPFLEAIRQMR
                     MDVGRDNVLYIHLTLVPFIRASEELKTKPTQHSVGKLREIGIDPDIILCRADRPLGEE
                     LKKKIALFCNVPAEAVIEALDAQTIYEVPLLLESQGLDELALMLLRQRSPRKDLSAWK
                     AMVEKIRTPQRQVTIAVAGKYVELKDAYKSIGEALVHGGLAHNAKVYVKYLDVEERHI
                     EAQLADVDGVLVPGGFGDRGVEGKVAVTRFARERNIPFFGICLGMQCVVIDVARNLLG
                     LKKANSTEFDPHTPHPVICLMENQKGVSAKGATMRLGVYPCKLNRNSLARKAYGRDVV
                     HERHRHRYEFNNRYRQRLESVGLRIAGVYAKKGLCEVVELKGHPWFVAVQFHPELKSR
                     PLRPHPLFRDFVGAALRRPAASPLRG"
BASE COUNT         2561 a         4325 c         4072 g         2229 t
ORIGIN      
        1 aaggaaaggg ccacggcccc gcccagcgcc ccttcgttcc ccctctcgag acgttccgag
       61 acccacggga tgaaccggct gttttgtcga cggaaccaga tgtccagatg gcggaccacg
      121 atgccgcacg ggatggccaa caagaggcac agcgtcaagg ttccccggcc cggatgggac
      181 gaaagctgac accacgtaat ggaaagagcc gccaccgcgg ccggatcgat gggccaatga
      241 ccgaccggga tcgggtgcac ccagaacatc tccaccgaag ccccggccaa caaccccaga
      301 ggcgcattgc cgagacacca tcccagcagc ggccccacca ccagcggacg ggacagcatg
      361 aactgtccga ccgcgatgtg gtccaacgcc aaaaggccgt agacccaagc caccgtcaga
      421 atctgtccca tgccctcccc gctcaacgcc atcactgcgg ccggatgtcc ttggcctccg
      481 gtgcggagga gcggttcaaa aattcccgga cgttgcgctt ttcgtcgttg gggagcgcgc
      541 ggacctcgac gtagacgccc ttcttcaaca ggaactcgat gtccgcccgg tcgtccgcgt
      601 tgagacacaa gaacggcgtc aacgccacac gccccgcggc ctcatgaaga ccgccgaggt
      661 tgagggtggc catctccacg ccggactcca ccagccgacg ggcggcgcgc acaccggcca
      721 tcagaaccat gacgcggtcg tccttcacgg cgtcgctctt gagaaaatcc gcggcgtcgg
      781 acaccttcaa cgccaccacg cgcacgtccg gcggcacggc cagccccatc agactcacct
      841 ggaaagggtc ctccgccacc tcgtcggaaa ccaccacgat gaggctcgcc tggatgacgc
      901 gaagccagcc ttccaccacc tggccgtgaa tcagacgatc gtcgatgcgc accaaaacca
      961 agggcatcag gcattctccc ggcggccggc cgtgcggagt ttctggacgg gccgctggat
     1021 ggtgcgcgga ccgtcgtccg tgatcttttg cgccaagagg tcgagcggca tgtagtgcct
     1081 cttggtcaac accgacacca gcatgggcag gttgacgccg gtcacgattt caaaattcca
     1141 ctgcgggtcc tggcactgac gcagggccga gttgcaggga gtgcccccca tcatgtccgc
     1201 caggaccaga acccccgccg ggttcttcat ctcttccagc accacccgga tcttcttgca
     1261 aaaagcgtcc ggcccctccg tcgcgtccag accgagtatc cggaccgcga cctgtttgcc
     1321 gacgatccct tcggcggtgc gcaagagttc ctcgccgatc cgtccatggg taacaatcaa
     1381 tacgccgatc atttaatcct ccggaggagg tggttcaaga gggccgcggg gacatcttcc
     1441 cgctcccatt cgatatcata tcttgcggtt cctgagcaaa tcgatcacgg cgccggccag
     1501 gcgcccggaa gaatgccgcg ccagggcggg aacggcgtcg cggcccctaa aactgggccg
     1561 caggtcggcg ccgatgatcc tcacccggcc gaaatggtct tgccggggcg gatcgaccgg
     1621 gtaggaatcg aaggcggcgt aatgccgcag gatcggttcc gggaacgcgc ggttgttcaa
     1681 caacatggca tccaccactc gctcgcggat gccggagccg caatgttcca agacggcccg
     1741 gagatggtcc accgccgaat agccgtcggt ctcgccgggc tgggacatca cgttgcacac
     1801 atagatcttc cgggcgcggg aacgggccac ggcctccgcc acgccgtcca ccagaagagg
     1861 cgggatcacg gatgtgtaga gagaccccgg accgatgaca atggcgtcgg cgtcccgcaa
     1921 agatttcaga acacccgggg ccgcctgggc ccgggacggc gtcaaataca catgctgaat
     1981 acgcgtccgg ctccgcgaga tccgtggttc accgagcacg cgtcggccgt cggcgaggcg
     2041 ggcatggaga cgcagatgct gcaggctgac cggaaggacc cggccgcgga tggccaagac
     2101 ctggccagcc gccgccatgg cgcggtctat cccgcccgcc acgtcgcaca accccgtgag
     2161 gagcaggttg cccagcgaat ggccggccaa ggaatcctgt ttcgaaccgc cgaccgcgga
     2221 atgagggaaa cggtattgga acagacgggc caccagggta tcgtcctgcg ccaacgccac
     2281 gaggcagttg cgcagatcgc ccggcgggag gaccccccaa tgccgacgca gccggccgga
     2341 gctccccccc tcgtcggtga cggtgacgac ggcggtgagg tcgctggtga actgcttcaa
     2401 accgcgcagg agggtggaca gccccgtccc tccgcccaaa acgacgatgc gcggcgattc
     2461 cattccgcga aacgaggaag gcggcaaact agccccgccg cgcgatgtcg cggtgctgca
     2521 cgcgcacggg cattttgagg tgcgtgcgga taaaatcggc cagggcctcc gccaccgcca
     2581 cgctccgatg ccggccgccg gtacacccca cggagaccgt cagatagctt ttgccttcga
     2641 ggacgaaccg ttccagcagg aattccatca tctggcggag gtgcgccatg aacttctgcg
     2701 tcgtcgggtc cctcaggaca tagcgggaca ccggattgtc cttgcccgtg cgggcgcgca
     2761 gttgtggcac gtaattcggg ttcggcagga aacggacgtc ccagaccagg tcggcgtcga
     2821 gcgggatgcc gtatttgtag ccgaaggaca tcacctggac cgtcatcccc tgcggatggc
     2881 gcagacccag cgtctgcacc acgatctcct tcacttccga cggggcgagg cgggaggtgt
     2941 cgatctgcca atccgccagc attttgatgt cgcgcaacag acggcgttcc cggcgcagcc
     3001 cgccgaggat ggtgcctccg agcggatgcc gccggcgcgt ctcggaaaaa cggcggagca
     3061 gggtggtgtt gtcggaatcc agaaaaagga cccgcagctg gaacccttcc cggcggaggt
     3121 tctccaagga acggcggaat tcgcgcgccg gcaggccggt ccggacgtcc acccccaaag
     3181 cgatacggcg aaggcgctgc ccccagccgc ggtaaaaccg gatcaacgag ggaaggaggg
     3241 gcaggggcag gttgtcgatg cagaaatagc ccaggtcctc caacgcgcgg agagcgacgc
     3301 tctttcccgc gccggacaac ccggtcacga tgacgcaatc atagcccccc cccgaaacgg
     3361 cgggacggga acccctgctt tttttcaccg gctcaaatct cctcgttgga aaaaatcagc
     3421 gattgggaaa cgggatccgg aacggaatgt gttggcgttt gggcgcggct taaaggcggt
     3481 cggaatgacc ggacattttc tggatcagac gctcgttcaa ttcgcgggcg gagtggatgc
     3541 cctcctgctt cagacgctga ttgagcgccg ccacttcgat caaagcggcg aggttccgcc
     3601 ccggatggat ggggacgcgg atctcgggca cgcgaacccc cagcaggacc gaatgctgtt
     3661 cttccagccc catccggtca tagttcttgt tctggtccca catctccaaa taaagcacga
     3721 gctcgatgat ggtccgtttg atgaccgccc ccacgccgaa aattttccac acgtcgatga
     3781 tccccagtcc gcgcacttcc atgtggtgct gaacgagcgg gctgccgctg ccgacgagga
     3841 tgccgccggg tttctggcgg atctccacgc ggtcgtcggc caccagcata tggccgcgct
     3901 tgaggagttc cagggcgcac tcggatttcc cgatgcccga actgcccagg atgaggaccc
     3961 ccatgccgta aacgttcacc agggtcccat ggacggaggt gtacggggcc aggcgctctt
     4021 ccagataggc ggtcagttcg ccgatgagac gggcggtctc ccactgggac tgaaaaagcg
     4081 gcaccttgta ccggtcgcac tgttcctgca tctccggaat cggctcgcgg ccgtgggtga
     4141 gaatgaggca ggggagatct ccggaagaga ggatgcggtc caggatatcc atccgctggg
     4201 cgctggccaa agtggcgagg aaggcgcatt ccccctggcc gaaaacctgg atacgttccc
     4261 ctcggtagta ttcgaagaac cccgccaacg tcagacccgg ccggttgatg tcggtgacgc
     4321 ggatctggcg gttcaacccg gactggccgg acaccaacga caaccgcagg gaggaagcct
     4381 gttccttcca gaactcctcg acgagcagcg gggcgggggt cccggaagcg ggattaatac
     4441 tcatcttctt cacgaatgat tttgaggacg tcttccggtg tcttggcgtc gcgcagggac
     4501 tgacggaaat atttgtcttt caacaagcgg gagatcttgg ccagggcctt gagatggttt
     4561 ccggcggcgt ccagcggcgc caccagcaga aatatgatgt agacggattc gccgtcgagc
     4621 gcctcgaagt ccaccccccg gcgcgagagg cccagcgcgg ccacctgctg tgtcacggaa
     4681 ttcaccttgc cgtgcgggat ggccaccccc tgaccgatcc cggtggaacc gagagcctct
     4741 ctttccatca acaccgagac catctgtttc ggatccggca gtttgttggc ggccgacaga
     4801 agcccgcaca actcctccaa gacttcctgt ttaccctgag ctttcaggtc cacagtgatg
     4861 acctcggggg caaggatatc ggcaagtttc atttatgggg tctcccggcg aattttacag
     4921 gttcgactca agcaacccgt aggttttgtc cttgcgctta taaacgacgc tgacgatgtc
     4981 gtcgttcatg aacacataga acatgtagtt cttctgttcc atttcctcga cggcctcctc
     5041 cacggtcatc ggacgggggg tgaaacgccg cacctgcgtg agcgggagcg cgaaggagtc
     5101 gtctccgtcc gaagggatca gggggatggg caacccgacg agggaccgtt tgctctgtcg
     5161 acggccgcgc acgcgccggt ccttctcacg cgccatgtgt ttatggagtt tttccgcgac
     5221 gaggtcgatg gccgcataga ggtccgccga ctcccccttg acgcggaaat cgtgatggcc
     5281 gttggcatgc acgacgatct cggcgatatg ccgatgtttc tcgacttcca ggacgacctg
     5341 ggcccggatc acggactcaa aatgccgccc cacccgttcc agccttttct gaacgtagtc
     5401 cgccaacgac ggggtcaaat ccaaatgccg cgctgtaatg tttacctgca tcaaacgccc
     5461 cttcccctct caactctcga aggaacaccc gcctccgccg aacggacgtc gggctccgac
     5521 gttaaaaatt cgtttttccg aatgaaaaaa tatagcaaac gtcggaaaga gtgtcaattt
     5581 ctccgccgga gaacggcgtt tttaaaggcg gaaacggtcc cccagataga cccggcgcgc
     5641 ctccggtgaa ttcaacaggt cctgggcgtt gccctgcacc aggatgcgtc cgctgtaaat
     5701 gatgtaggcg cggtccacga tctccaacat ggcctgcacg ttgtggtccg tcaacaacag
     5761 ccccagcccc ctcttcttca gggcgcgcag gacttcctgg atgtcgttga cggcgatggg
     5821 gtcgatgccc acgaaaggct cgtccagcag aagataccgg ggatcggtgg tgagggcccg
     5881 ggcgatctcc gtgcggcgtt tctcaccgcc cgaaagggtg taggccatct gttttctcaa
     5941 acggtcgagc ccgagctcct gcaggagttt ttcgcatttg ttcttctgtt ccgacggatc
     6001 gaggggcagg ttctccagga tggccatcag gttgtcctcg acggtcattc ggcggaagat
     6061 ggagggttcc tgacagaggt atcccaagcc ggtgcgggcc cggcggtaca tgggcaggtc
     6121 cgaaacgtcc tggccgtcga gcgtgatagc gccgccgtcc ggacggacca gccccaccat
     6181 catgtagaag gaagtcgtct tgccggcccc gttgggcccg aggagaccga cgatctcgcc
     6241 ggggttcacc tccaacgaga caccgtccac gacggtgcgg acgccgtagc gcttcttcag
     6301 tgcgtcagcg tgcaggggca tggacatcct ccaagggaga gtcgggcgtc aggatggtgg
     6361 cccggacgtt cctttcgaca accagacgtt tgtcgttcaa gaaataggtc atctcgtcgc
     6421 cgctcaaatc gcggatctcc tccccttccg tctggcgcgc ccgcggctgg accggcttcg
     6481 gatccgcggc gcgggagtcg ttccgaaacg agtcgtagcc gccccaaaaa cggacattct
     6541 ccatcgaccc gtcgtaaaaa gcatcgtgcg cccagatctg ggtgtcccgc accgtcgggg
     6601 tggattcctt catccacacg tagaccaatc cctccgcctc aacgcacgtc caggcggagg
     6661 tgctttgcgt ggcggactcc acgacccggt cgtaaaaggt gaaccggagg gcctccatct
     6721 ccatgcgctg ggtctcggtg ctggcggaga ggggcgagcg gtggaccttg accggtttcc
     6781 ccctccccca caacgtgccc gaggaaatct gggaggaata cgtcccctcc tcggcccagg
     6841 attcccagcg gacgccgccg agcgggtcgt cgcgccgcag atagacgcgg ccccaggctt
     6901 ccgtgaggcc ctttttctct tccgacacca tccggtcggc cttcatgaaa tcgttccccc
     6961 gaaccagggt gacgcctccc acgaactcca ccgccgcgcc cttgcgaagg aggttcatcc
     7021 ggtcgccggt gatgacggtc ttgggaggct tcggctcttc cgtcaaaccg gcgatcgcca
     7081 gccccaagac aaccgacacg gccagcgcga agggcgaaat cctgttcatt tgtccttgcc
     7141 tccgcccttt ccgccggcga ggggggtctc gccgacgatc tccacccgct gctgacggac
     7201 aagcacctcg gaaaggtccg gcgtggcctc ccagccgacg ccctggatgg tcgtccccgg
     7261 cctcacgatg gtcaccgggg cggtggagac gatcctctcc ttttcgctga tgtagtgcag
     7321 ccattccgac gtcaaccggg cgccgtcggt ggaggtcatg accacctggt cccacgccca
     7381 caggtcccgc gcgccggagg tctggatttc gccggcgccg gcttccaggg aagaactggg
     7441 ctcgctgttc tcaaaaaacc ggatcgacgg cttctggatg cggatgcgat tttccgactc
     7501 aaaaatatcg gcccggggag actccagatg ccagtctttt tccttgcccg cgaatgaatc
     7561 gatcgaaaaa ttccggatga cctggtcggg cgaaacggcg gccgcttttt cgacccgccc
     7621 cgccggatgg gaagaacggc aggaggccag aaacccgccc cccaggacga tcgccgcgac
     7681 gaaaacgaga aaccggaacg gcttcaacgc cgtttcttgc cctgggcgtc cttgaaaaga
     7741 cgcgactggg gacgttcaaa ttcggcgacg accttcctca acagtccctg ggaccgcagg
     7801 acgaaatcga cggcctcgcg gaagacgccg cgtcctcccg gagcccgcgt caccagcgag
     7861 cagacgcttt tcacttcggc cacggcgtcg ctggggcaga cagccaaccc cgcccctttc
     7921 agggcggcga tgtcgatgag gtcatcaccg atgtagaggg cctgccgcgc ggtgagacga
     7981 aaacgacgga ggatgtcctg cagcgccgcg cgcttatcga ggcaagactg atacagcgcg
     8041 tccaccccca actcctcggc gcgggccttc acctgcgccg acgcgcgccc ggtgatccac
     8101 gccacgtaaa accggcggcc gctcttctcc agcatggaga aagcgatccg gtccttgacg
     8161 ttccagacct tgacctcttc gccggacttc aaaaggacga tggaaccgtc ggtcaagaca
     8221 ccgtcgacgt ccatggccag gaggcggacg gcggcggcgc ggcgccggag ggaagagcgg
     8281 gtcctcatgg cgttaaccga gcccctcttc caaaaggtct ttctcatcca aaatgccgac
     8341 ggggcggccc gtccggtcca ccaccgggag gttgtcgaga ccgaaacgcc ggaagaggag
     8401 ggcgacgtcg gcggccaggt ctcccggagc gacggtgcgc ggggagcgcg tcatcacctg
     8461 ccgcaagggg cggtccagga ggcgatcgtc cttctgcagg ccgcggcgga ggtctccgtc
     8521 ggtaaaaacg cccgccaagc gtccgcggcg gtcgatgacg gaggccgcgc ccatgcgcgt
     8581 cttggtcatc acccagaggg cctcccgcac cttctgcgaa tcggccacga gcgggttctc
     8641 cgagcccttg tgcatgatgt cggccacacg gagcgtgagt ttttttccga gcgtcccgcc
     8701 gggatggagc cgggcaaaat cttccttgtc gaaccccttg agctccatca ccagcatggc
     8761 cagggcgtct cccatggcca gcatcgcggt ggtggaggcc gtgggcgtga tgttgaacgg
     8821 gcaggcctcc ttctcaacgg ccacgttgag cacccagtcc acggaacggc ccaacatgga
     8881 gcggggtcgg ccggtgaggg cgatcaacgg gagttttcgt tggtggacga cggaaagcac
     8941 ccgcttcaat tcctccgtct cgccggaata agacaacacc agaacgatgt cgcccgtcga
     9001 aaacatgccg gaatcgccgt gcagggcctc ggtgggatgg acgaagaaag cgggcgttcc
     9061 gacggaagcc agggtggcgg ccagcttgcg gccgaccagc cccgatttgc cgatccccat
     9121 gaccaccacg cggccgcggc agcgggaaag gcaccgagcg gccaggagga aatgctcgtc
     9181 tatgtggcgc agttgatggg tcaccgcctg ggcttccagc cgcagggtgc gctgaacgcg
     9241 acggcgccaa tctttcaatt ccctgatctt cattttaatg gcacgcacct ccaaaaatta
     9301 ttcagtgagg ggccggcggt ttgggcgcgt ggccgtcccg ggacgaacgc aggtggtcct
     9361 cgatgtcgac gaacagacgg atgaactccg ccacggcctg aaaagccacc aggagaaggt
     9421 agccggccag ggcggcggcg ccggcgaaaa agaccgccac cagcagactc ttgttccaat
     9481 aatacgccag cacccccaac gcgcacagtc cgccggcccc ggcggaaagg cgggccaggt
     9541 aacgcgcccc gttggagaaa cgggccagcg cctcgtagcg gggccgctgg aacggctccc
     9601 accgttccag gaacgaatcc ccgcaccgct cgcaaaaatt atgatccggt tcgttctcgg
     9661 cggaacactg cgggcagacc ttcatttcac ccctccccgg cggcccatga agctgaggag
     9721 gtttatccac cgccgtcggc cgaaccggcc gctgatggcc accccgcccc agaggagcgc
     9781 gcccaggatg gcgccggaaa ggaactccac tccgcgggcc gtggaccagc ccggttcagg
     9841 gaactgcacg gccatggcca ccgagaaatt ccacgccacc gcccccgcca gactgcccac
     9901 aaaaaccccc ccacggtctc ccatcctttc aaccggccga cggaaaaacc gatcgccatc
     9961 ccgaacaacg cccacaccgc gacatgcagc acgaagagag cgtcgggcga ggcgccgcgc
    10021 gccagaaaac gggccgccgt gccgaaggac agtcccgccg cgccgcccgt gaagagcccg
    10081 cgccacacac gggaggagga gcgttccatc aggccgccgg ccatccccag gaacaacccg
    10141 gccaccgtca gaaaaagcgg atgcggcagg gacaggtcgg cgccgggcag ggtgagccgc
    10201 aaaaacgtgc ttcccaggac ccaaccgacc ccgcccgcca ccgccccgcc gatggaccgc
    10261 cgcagggacc aggtgaaatc ccaaagagcg atggagacgc cgcaatggcc gcagtaacgg
    10321 gcgcgatcgt gcagcgaggc accgcactgg ggacaaggga aaagaaaacc cgttcgttcg
    10381 ctcatgaccg gccctactgt ttcagccagg gggtcatttc cttcggaatg tctctcatgc
    10441 gatacggctc cggcaccttg gaaaggacca gactgaggac gaaatagatc acggtcaacg
    10501 acacatagat gacgagcagc gctttggcat gccagcgcca atccggtttc cagaagttgt
    10561 ccgtcgacag atcgctccgg ccggcgaaat cccgcggggg ggccgccggg gcgattttcg
    10621 cccccttcgc ggaggacgag gacagcgggg ctccgcagtc gttgcagaat ttggcgttgt
    10681 tcttgttctc aataccgcat ttcggacatt tcatcccaaa tcctccggaa cggggaatta
    10741 attgttgaca aaccgtttaa cgacgcggtc gatggccagg agccggcgca gcagcggttc
    10801 aagttcgcgt agggggacgg cgttggggcc gtcggagagg gcgtgttccg gccggggatg
    10861 cacctcgaag aacaccgcgg ccacgcccac cgccaccgcc gcccgggcga gaggcgcgat
    10921 gaattcccgc tggccgccgc tggaatcgcc ccgtccgccc ggaagctgca ccgaatgggt
    10981 ggcgtcgaaa atcacgggcc ggccgaagga tttcagcacc tccaggctgc gcatgtccac
    11041 cacgagattg ttgtacccga aggacacccc cctttcggtc aaaaagaggt cgcgatggcc
    11101 gatggactcc gcttttttga ggatgaggcg ggcgtcccag ggcgacagga actgaccttt
    11161 cttgacattg accggcaatc cggtgcgggc ggccgccagg agcaggtcgg tctgccggca
    11221 aaggaacgcc gggacctgga gaacgtcggc cacctcggcg acgcggggca cctcgccgac
    11281 ggaatggacg tcgaccagga gcggcaggcc gaactcggac ttcacccgcc gcaagacctt
    11341 caacccccgg gccaacccgg gtccgcggta ggaatcgacc gacgaacggt tggccttgtc
    11401 gtaggaacac ttgaaaataa gcggcacgtt cagacggcgg cgcacggcgg ccagctcggc
    11461 cgcgatccgc agcgtcactt tttcgctttc gatgatgcac ggaccggcca ccaccgccag
    11521 cgggcggtcg ttggcaaggc ggcaacttcc gatctttatc tcccgctgtt gggacatggg
    11581 gtcaccctct cagagggctc gccgccggcc ggcgcagggc cgcgcccacg aaatcgcgaa
    11641 acaggggatg cggccgcaac ggtctggatt tcagttccgg gtgaaactgg accgccacga
    11701 accaagggtg ccccttcagt tcgaccacct cacacaaccc ctttttggca tagacgccgg
    11761 cgatgcgcag accgacgctt tccaaccgct gccggtagcg gttgttgaac tcataacgat
    11821 ggcggtgccg ttcgtggacc acgtcgcggc cgtaagcctt gcgcgccaag gaattccggt
    11881 tcaatttgca gggatagacc cccaagcgca tggtggcccc cttggcggag accccttttt
    11941 ggttttccat gagacaaatc accggatgcg gcgtatgggg atcgaactcc gtcgagttcg
    12001 ccttcttcag ccccagcagg ttccgcgcca cgtcgatgac cacgcactgc atgccgagac
    12061 atatgccgaa gaaagggatg ttccgttcgc gggcgaaacg ggtcacggcg accttcccct
    12121 ccacgccccg gtccccgaat ccgccgggaa cgagcacgcc gtccacgtcg gcgagctgag
    12181 cctcgatgtg ccgctcttcc acgtccaaat atttcacata gaccttcgcg ttatgggcca
    12241 acccgccgtg aacgagcgcc tcgccgatgg atttataggc gtccttcagt tcgacgtatt
    12301 tcccggccac cgcgatggtg acctggcgct ggggggtcct gatcttttcc accatcgcct
    12361 tccaggcgga caaatccttg cgcgggctcc gttggcgcag aagcatcagc gccagttcgt
    12421 ccagcccctg gctctccaac agcagcggca cctcatagat cgtctgggcg tccaacgcct
    12481 cgatcaccgc ttccgccggc acgttgcaaa acagcgcgat cttcttcttc agctcctcgc
    12541 ccaacgggcg gtcggcgcga cagaggatga tgtccggatc gatgccgatc tccctcagtt
    12601 tcccgacgga atgctgggtg ggtttggtct tcagctcctc ggaggcccgg atgaacggga
    12661 ccagcgtgag atggatataa aggacgttgt cccggccgac gtccatgcgc atctggcgga
    12721 tggcttccag gaagggcaag ctttcaatgt cgcccacggt gccgccgatc tcggcgatga
    12781 gcacgtcccg tccgcgcgcc acccgtttca ggcggttctt gatctcgttg gtgacgtggg
    12841 ggatgacctg gacggtattg cccaaaaact ccccccggcg ctcctgctgg atgatggtgt
    12901 cgtagatctg gccgctggtg cagctgttct cgcgcgccat gtccatgtcg aggaaccgct
    12961 cgtaatgacc caggtccagg tccgtctccg ccccgtcctc ggtgacgtaa acctccccgt
    13021 gttgataggg gctcatggtg ccggggtcca cgttgatgta cgggtcgagc ttgatcatgg
    13081 tgacgttcag cccgcgggat ttcagaagcc gtcccaaaga agccgccgaa atccccttgc
    13141 cgagggaact caccacgccg ccggtcacga agatgtagcg cgtcatt
//
LOCUS       DAOTFD010000054        13053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3324_length_13053_cov_3.596015, whole genome shotgun sequence.
ACCESSION   DAOTFD010000054 DAOTFD010000000
VERSION     DAOTFD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_13053_cov_3.596015"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..7238)
                     /locus_tag="P5079_04900"
     CDS             complement(42..7238)
                     /locus_tag="P5079_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY29360.1"
                     /translation="MRVYNLAGAETTAEDVFYVKKDNVFPTISDNQAGDFNWRNSSGT
                     IYDVDFQDDASLLNSAQYRVFTGPGQTGAERVPLTPIFDSAALAAYADDWPLSSAAFD
                     ALLEGATNYVSLRVEDNAGNVSSLTDAFLVFKDTTAPTITDNQMGDDTTYYANPGAVF
                     DVDFNDAGGSLLKNLQFRVFSEAGQSGTLIKDWTEIALTVNATYYDQEWGIGFGALQL
                     GKNYVSVRAFDHAGGSVTQSDVFYVFKGTGPPNIIDNQAGDFVWRRQDGTLYNVAFSS
                     NSDAYALDYFQTRAARAPIQQSLIQDWTTAVFGISAAEYTDPWALPGGTWDLLEEGTN
                     YISVRVVDVSGSSGSASDVFFVLKDTTPPSITDNQPGDAVWRRAEGTLYDVDFHDLAS
                     GLTTVQYTVSSAAGLSGGVGDVLPWTTIASGLDGAPDYDTDWPAAFSSLTEGTNYVSV
                     RAHDRVEFVKVSTDVFTILKDTTPPTISNAQGGDDFWRIANTGLYDVNFLDSGGSQLA
                     RFEIRVSTMSGDVGPFLVDFTTIVASINAATYTADWSLPNAVFDALPNGPTNYVTVRV
                     FDVAGSSDLVVDAFYVLKDTVPPVVADNQPGETLWRDTDAGPVYDVDFSDLQSQLTTV
                     QYTVSSSAGLSGGEGDIVPWTDIAAPPVSATSFSSDWAVAFASLPEGRHFVSARAYDV
                     AGNSATAVDVFEILKDTSPPVVTDLQTGDTVWRRSPDTTYDVDFSDLYSSLATAQYTV
                     SATPGLSGGEGEIVPWSDIAVGIDSTSYTTDWSVNFSAFTEGATNYVSVRAFDALNHV
                     TTGQDVFFVLKDMSKPTIQNLVVGDDTWRKDPGTLYDVNFYDSGGSHLDTVKYTVHAV
                     PGLAAGEGNLVPWTTIATGIGTDAYTDDWPIAFSALPEGQSYLSVRAWDVTGDTETLT
                     DAFYVRKDTSAPTVTDNQSGDTAFRSSNNGSYDVDFADAGGSQLALFEIKVSTLPGGA
                     GPYLVDFTTVVANINAASYTAAWSLPNAVFEALFGGGTTNYITVRVWDAAGSSTAASD
                     VFYVKKDTEPPMTVDNQEGDDTWQRTGGTLYNVDFQDAGGARLDRVQYTASSGPGLPG
                     GVGDLVPWTDIASSLNALTYTTNWGVAFSSLTSGSNWISARSIDNAGNVSSVATDVFY
                     IKKDTQPPSGTAMAPAAAKTVNLEVPYAVSDTGPAGVQHVKLYYTTQQQTPYAWTPWG
                     TTFTASPISFTAAGEGTYGFRIVAYDNANNTDESVPPASTTAPESSTVVDMTLPEIDD
                     LQTGDDVWRGAAGTVYNVDFRDPGGSRLTGAQYTVDAAPGLSSGEGGVVPWTGIFSSA
                     GTETYAADWTVDFSALKSSHNHVSVRTYDLAGNTRTLTDVFYIKKDTIAPAAVDNQTG
                     DDLWRRTAGTAYNVDFTDEFSLVNSVQYTAASSPGLPGGVGDRIPWTTLATGLSSSAY
                     TNDWTVNFPLLQNGTNYVSVRGYDAVGNTQVTSDVFYVRKDTTPPSVTDNQPDVAAYT
                     NAAGAVYDVDFVDTGGSGLNHIQYIVRTGTSPSSAVIVDWTDIADAPLGTASHSSDWP
                     LAFSLLQEGTNYVSVRAWDMAGSTATTDNVFFMRKDGVGPTVLDNQTGDTAWRASNTG
                     LYDVDFQDVLSGVASFQIKTMSGPLLTGATLQDWTTVLTTNTTTYTANWSLPAGTFNL
                     MNVGDNYVSVRAFDGAGNTGPASTDAFFVKKDTAPPTATDNTAGDDTWRSTDPGAVYD
                     VDFNDPHSLLSSLQYTVHSQPGLPGGIGDVVSWTTFAGGVNAADYTAAWGLDFPLLQP
                     GTNYVSVRAQDGVGNLSSASVDVFHVWKDTVAPAAISTLASSSGAEGEIALSWKAPAD
                     DGATGDPVTAYVVKYATTQYGGQIDFDNATPFDQNWTILSPGETQNETVTGLTPSTLY
                     FIAIEAVDKAGNQALLSNLSGNSANAGSDATAPSAVNDLAAYTGTLPGEIQLTWTAAG
                     DNGTSGTAAGYVVKYRTSGAIATDTDFNTATTFSQSWSPVPAGEPENRVVTGLSEGTT
                     YYFAIKTQDEIPNTSGLSNSPSAKAGEPGAADGTFTFGQGTNNYPSYRSWASSSFGSI
                     LNGQTATATQRWHVLRSNPRNRKEKLLGILSSDGTLTVQRYNGDTGNWNNEWTATIGG
                     TNSAYRGFDIAYEHASGRGMVVYSNNSNNLYYRLWDGSSWSVAASTDVPGSGGIFRWI
                     QAKPHAATDEIMVTALDANSDIFAVRWTSSAWANSQTLTTSGGSATMECFNGAWESNS
                     GDFVVVYGTSTNTEYRVWNGSAWSSGAAGPALGGSVAYYLRVEADPSSDKIGFTSVDS
                     AGDWNAAIWDGSLWGNMPTEDTAIIAGTNGRTVDLAWEKTTGKLIVAAINSGTPARTR
                     ITWATCSSKTLSASPPEAGASCARAAAPRSAKSR"
     gene            complement(7181..12595)
                     /locus_tag="P5079_04905"
     CDS             complement(7181..12595)
                     /locus_tag="P5079_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29361.1"
                     /translation="MVIRNAYLELEALASVSTTLSDLQIRFNTGGTASTLVFDGAGTY
                     ITQTGELFRVAAKADATAQVTAWSNQTYSASVTLTGPSSNMHALKLYVTYEYDDTSAT
                     QVKTVRFPAYYASNVTSAQSQLTGGSTTAFTYNAEIADSGVAVQQQWFEIRGQRQSNS
                     STADGNLRCRIGSDTTEPVLFFEGAGTVTYDFLYLTQAQTSVPGFAANTSQTLNIVSG
                     GNANITVLNGECVVTYTFNGNSATKTKTVAYFLGQSPGAAAAGNILQDLFLLEGNITL
                     KRLYARISSTFDVATLTNFVLDSAVAGQSVTQRSYSTLGQAATISGYSFLHDLTEQAG
                     SWVNGSSVTLSYPAQTGRGGTGVELIATYDYTNEGAFTESYSVFAGQSSQQTTTSQAQ
                     NFTVYFPTATTPLGTKTLRSAYLQDRFNNSQAAGADRDTLINVGGTNSQTVDHQHPAE
                     AFTATVLYTSTNQVTPTNAVTYTGNHDVTSTDRAVFSNKAVVSYTYFPPPRPPTGLTP
                     YKSDGTTVVSTGQWINDGTLVFKFSMDAPPLADTLTPEIEIKPVGQAFDGTGTQTGVA
                     QAYSGSVLTGSVTVSGLSTNTTYHWQARVQGTGGNGAWVSYGGNAESASDVGIDLTSA
                     PAPAMTAISPIQDARLSTGTLVLDFENVSDGAGSGVQNYFVQVSTDINFGVLSFSSSP
                     TDSQASVTDLSQDFYYWRGRTVDVAGNTGDYSSPKTFRVDLTTPTVSDLQSGDDIWRS
                     AAGTTYDVDFYDLGGSSLSYVQYRVFSGPGQTGTLLKDWTTLALGIGATYYTADWPVD
                     FAALQETSGNYVSVRAVDFSTHTAAAVDVFYVRKDTTPPSVPLLSSPADNVNLNSGNI
                     LFDWTDSTDASSLVADYVLEVATDPAFTAGSIVFSSSTALSQASTATLAQGFYYWHVR
                     ARDNAGNSSSNAVLRTLRVDLTTPTVSDLQAGDDAWRNAAGATYDVDFYDLGGSSLSY
                     AQYRIFSAPDQSGTLLKDWTAIALGIGATHYTADWPVDFAACREGTNYVSVRAVDLST
                     NTSTYADVFHVRKDTTSPPTPTLLSPADNVQNNHGNISFDWSDVSDGASGPADYVLEV
                     STRDDFGILSFSSSPIVSQASTTSLSTGLYYWRVRARDNAGNYSADTSTFSFRVDRAS
                     PAVTDPVPGDDTVWRSANPGAVYNVDFADTGGSRLARFEVKASTLPGGMEPSLVGYTT
                     VVSDINADSYSMDWALPVLFFNALVSHATNYITVRAFDTVGNISAESTDVFFVLKDTV
                     PPATPSPLSPADGSVTKDPRPAFDWSDAPDVPSGVDHYELQVSTDPAFGTLSYSPSPA
                     VSQATPAADLPTATYYWRVRAVDKAGNAGDYSSPSYEILVDTIAPVIQDSQADVAAFL
                     NAPGASFNVDFADAGGALLDTAEYRITLGPNQTGTVRKEWTPIFVGLHLAAYETDWQV
                     DFSALEEGETTNYVSVRVWDTAGTTTTLNDVFYVNKDVSLPSVIDQQTGDDTWRTADP
                     GAIYDVDFRDLVSRLDRAEYRVTSLSGGGGTEIIPWTTLFGPNHGSTEYTTDWGIDFA
                     ALSQGQNHVSVHVWDKAGNDRTFTDVFYVRKDTQAPTLVDNQPDDDTWRTANTGSYDV
                     DFHDDPGGSLLEKFQIRASTVAGGTGPFAPDWSDVATGLGAADYTANWSLPSGVFETL
                     PPGKNYISLRAFDAAGNAGAVLTDGFYILKDTSPPQIADAQSGDDAWRRFGGTAYDVD
                     FHDPDSLLTQAQYTVWTGPALTGTEKIPGRPFSLPPGRPPTRRTGPSTSTAWPRASIT
                     SRCASTTWPGQRRPRKTSSM"
     gene            12621..12716
                     /locus_tag="P5079_04910"
     CDS             12621..12716
                     /locus_tag="P5079_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRY29362.1"
                     /translation="MVGGVQAVPEPASVALIALGLAGLGLRRRRG"
     gene            complement(12779..>13053)
                     /locus_tag="P5079_04915"
     CDS             complement(12779..>13053)
                     /locus_tag="P5079_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY29363.1"
                     /translation="QTLFANPGCRVERIVSHGHASAPDDWYDQHGDEWVVLLAGAAVL
                     CFDGGDDVKLKAGDWITIPAHCRHRVESSSADALWLAVHCAAGASK"
BASE COUNT         2157 a         3818 c         4657 g         2421 t
ORIGIN      
        1 atcaccgggg tcagttcggc cgggccgctg cacctgcgcg gctacctgga cttcgccgac
       61 cgcggtgcgg ccgccctcgc gcacgacgcg cccgcctcgg gcggcgacgc ggagagcgtc
      121 ttcgaggagc aggtggccca ggtgatccgg gtgcgggcgg gtgtgccgct gttgatggcg
      181 gccacgatga gtttccccgt ggtcttttcc caggccagat ccacggtgcg gccgttggtc
      241 ccggcgatga tggcggtgtc ctcggtgggc atgttacccc agagactgcc gtcccagatg
      301 gcggcgttcc agtcgccggc ggagtccacc gaggtgaacc cgatcttgtc cgaagagggg
      361 tcggcctcca cgcggaggta gtaggcgacg gaccctccca gcgccggtcc ggcggcgccc
      421 gacgaccacg ccgatccgtt ccagacccgg tactccgtgt tggtcgaggt gccgtagacg
      481 accacgaagt caccggagtt actttcccag gcgccgttga aacattccat ggtggcgctg
      541 ccgcccgagg tggtgagcgt ctggctgttg gcccaggcgg aggaggtcca tcggacggcg
      601 aaaatgtcgc tgttggcgtc gagggcggtg accatgatct cgtcggtggc tgcgtggggt
      661 ttggcctgta tccaacggaa gatgccgccg gatccaggca cgtccgtgct ggcggcgacc
      721 gaccagctcg agccgtccca gagacggtaa tacagattgt tgctgttgtt gctgtacacg
      781 accatccctc gaccggacgc atgttcgtag gcgatgtcga acccgcgata ggcggagttg
      841 gtcccgccga tggtcgccgt ccattcgttg ttccagtttc cggtgtcgcc gttgtaacgt
      901 tgcacggtga gcgttccgtc ggacgagagg atgcccagca gtttttcttt ccggttgcgc
      961 gggttgctcc gcaacacatg ccatcgctgg gtggcggtgg cggtctggcc gttcagaatc
     1021 gaaccgaaac tcgacgacgc ccacgaacgg taactggggt agttgttggt cccctggccg
     1081 aacgtgaacg tgccgtccgc cgcgccgggt tcccccgcct tggcggaggg tgagttggac
     1141 aggccggagg tgttggggat ttcgtcctgg gttttgatgg cgaagtaata ggtcgtccct
     1201 tcgctcagcc ccgtcaccac ccggttttcg ggttccccgg cgggaacggg gctccaggat
     1261 tgggagaagg tcgtggcggt gttgaaatcc gtgtcggtgg cgatggcgcc ggaggtgcgg
     1321 tatttgacga cgtaccccgc ggcggtcccg ctcgtgccgt tgtccccagc ggcggtccag
     1381 gtgagctgga tttcccccgg cagcgtgccg gtataggcgg cgaggtcgtt gacggccgac
     1441 ggcgcggtgg cgtccgatcc ggcgttggcg ctgttcccgg acaggttgga gaggagcgcc
     1501 tggttgcccg ccttgtcgac ggcttcgatg gcgatgaagt acaaagtgga cggcgtcagg
     1561 ccggtcacgg tctcattctg cgtttccccc ggcgacagga tggtccagtt ctggtcgaag
     1621 ggggtcgcgt tgtcgaagtc gatctgcccg ccgtactgcg tggtggcgta tttcaccacg
     1681 taggcggtca cggggtctcc cgtggcgccg tcgtccgccg gggctttcca ggagagcgcg
     1741 atttccccct cggccccgct ggaggaggcc agcgtcgaga tggccgccgg ggccacggtg
     1801 tctttccaaa cgtggaatac gtccaccgag gcggaggaga ggtttccgac gccgtcctgg
     1861 gcgcgcacgc tcacgtagtt cgtgccgggc tgaaggagcg ggaagtcgag cccccaggcc
     1921 gccgtatagt cggcggcgtt gaccccgccg gcgaacgtcg tccaggagac cacgtccccg
     1981 atccctcccg gcagcccggg ctgggaatgg accgtgtatt gcagactgga gagcagggag
     2041 tgcggatcgt tgaagtccac gtcgtagacg gcgccgggat cggtggaacg ccaggtgtcg
     2101 tcccccgccg tgttgtcggt cgccgtcggc ggcgccgtgt cctttttgac gaagaaagca
     2161 tccgtcgacg ccgggcccgt gtttcccgcc ccatcgaagg cccgcacgct cacgtagttg
     2221 tcgcccacgt tcatcaggtt gaacgtcccg gccggcagcg accagttcgc cgtgtacgtc
     2281 gtcgtgtttg tggtcagcac ggtggtccag tcctgaaggg tggcccccgt cagaagcggc
     2341 ccggacatgg ttttgatctg gaacgaggcc acgccggaga ggacgtcttg gaaatccacg
     2401 tcgtagaggc cggtgttcga cgcgcgccac gccgtgtcgc ccgtctgatt atccagaacc
     2461 gtcggcccga cgccgtcttt tcgcatgaag aagacgttgt ccgtcgtggc ggtggagccg
     2521 gccatgtccc aggcgcgcac gctcacgtag ttggtgccct cctgcaacaa ggaaaacgcg
     2581 agcggccaat cggaggaatg agaagccgtc cccagcggcg cgtcggcgat gtccgtccag
     2641 tcgacgatga ccgcgctgga ggggctggtc ccggttcgga cgatgtattg gatgtggttc
     2701 aatccggacc cgccggtgtc gacaaaatcc acgtcgtaga cggcgcccgc ggcgttggtg
     2761 taggcggcca cgtcgggttg gttgtcggtc accgacggcg gcgtggtgtc cttgcggacg
     2821 taaaagacat cggaggtgac ctgggtgttc cccaccgcgt cgtatccgcg cacggagacg
     2881 tagttggtgc cgttttgcag cagaggaaaa ttcaccgtcc agtcgttcgt gtaggcggag
     2941 gaggacaggc ccgtggcgag ggtcgtccag gggatccggt cccccacgcc gccgggcagc
     3001 ccgggagacg aggcggcggt atactgcacg gaattcacaa gcgagaactc gtcggtgaag
     3061 tccacgttgt aggcggttcc ggcggtccgg cgccagaggt cgtccccggt ttgattgtcc
     3121 accgccgcgg gggcgatggt gtcttttttg atgtaaaaaa cgtccgtcag cgtgcgggta
     3181 ttgccggcga ggtcgtaggt ccggacggag acgtggttgt ggctggattt gagagcggag
     3241 aagtcgaccg tccagtcggc ggcgtaggtc tccgtgccgg ccgagctgaa aatgcccgtc
     3301 cacggcacca cgccgccttc gcccgaggag aggcccggtg cggcgtccac ggtgtattgc
     3361 gccccggtga gcctggaacc gccgggatcg cggaagtcca cgttatacac ggtccctgcg
     3421 gccccgcgcc agacgtcgtc ccccgtctgc agatcgtcga tctccggcaa ggtcatgtcg
     3481 accaccgtgg aggactccgg ggcggtggtg gaggccggcg gaacggattc gtcggtgttg
     3541 ttggcgttgt catacgccac gatccggaac ccgtaagtcc cctccccggc ggcggtgaaa
     3601 gagatggggc tcgccgtgaa ggtcgttccc cacggggtcc aggcgtacgg cgtctgttgt
     3661 tgcgtggtgt aatagagttt cacgtgctgc acgccggccg ggccggtgtc cgacacggca
     3721 taaggcacct cgagattcac cgtcttcgcc gccgccgggg ccatcgccgt cccggacggg
     3781 ggctgggtgt cttttttgat gtagaagaca tcggtcgcca cggaggacac gttcccggcg
     3841 ttgtcgatgg aacgcgcgct gatccagttg ctccccgacg tcagagagga aaaggcgacg
     3901 ccccagttcg tcgtgtaggt gagggcgttc agggaagagg cgatgtccgt ccacggcacg
     3961 agatccccga ccccgccggg aagccccggc cccgaggacg cggtgtattg aacgcggtcc
     4021 agtcgcgcgc ctcccgcgtc ctggaagtcc acgttgtaaa gcgtgccccc ggtccgttgc
     4081 catgtatcgt cgccctcctg attgtccacc gtcataggag gttcggtgtc ttttttcaca
     4141 taaaacacgt cgctggccgc cgtcgaagac ccggcggcgt cccacacgcg gacggtgatg
     4201 tagttggtgg ttccgccgcc gaagagcgcc tcgaagacgg cgttcggcag ggaccacgcc
     4261 gccgtgtagg aggcggcgtt gatgttggcc acgaccgtcg tgaaatcgac gagatagggc
     4321 cccgcgccgc cgggaagcgt ggaaactttg atttcaaaaa gagccaattg gctcccgccg
     4381 gcgtcggcga agtccacgtc ataggacccg ttgttggagg agcggaaggc cgtatcgccc
     4441 gactggttgt ccgtcaccgt gggcgccgag gtgtccttgc ggacataaaa ggcgtccgtc
     4501 aaggtttccg tgtctccggt gacgtcccag gcgcgcacgg agaggtaact ttgaccttcg
     4561 gggagcgccg aaaaggcgat cggccaatcg tccgtgtagg cgtccgtccc tattccggtc
     4621 gcgatggtcg tccacggcac gaggttccct tcgccggcgg ccagccccgg aacggcatgc
     4681 acggtgtatt tgaccgtgtc cagatgggag ccgccggaat cgtagaagtt gacgtcgtag
     4741 agggtcccgg gatcttttct ccaggtgtcg tcccccacca ccaggttctg gatggtgggt
     4801 ttggacatat ccttcaatac gaagaacacg tcctgcccgg tggtgacgtg gttcagcgcg
     4861 tcaaaggcgc gcaccgatac gtaattggtg gccccttccg tgaacgcgga gaaattcacg
     4921 gaccagtccg tcgtgtagga ggtcgaatca atgccgacgg cgatgtccga ccaggggacg
     4981 atttcgccct ccccgcccga aaggcccggc gtggcggaga cggtgtactg cgcggtggcg
     5041 aggctcgaat agaggtcgga gaagtcgacg tcgtaggtgg tgtccgggga gcggcgccag
     5101 accgtgtcgc cggtctggag gtcggtgacg acgggcggcg aggtgtcttt gaggatttcg
     5161 aaaacgtcca cggcggtggc gctgttgccg gccacgtcgt aggcgcgcgc cgaaacaaag
     5221 tgccggcctt cgggcaggga ggcgaacgcc accgcccaat cggaactgaa ggacgtcgcc
     5281 gacaccggcg gggccgcgat gtcggtccag ggaacgatgt ccccttcccc gcccgagagg
     5341 ccggcggacg acgagacggt gtactgcacg gtcgtcaact gagactgcag gtccgaaaag
     5401 tcgacgtcgt aaaccggtcc cgcgtcggtg tcgcgccaga gggtctctcc gggttggttg
     5461 tccgcgacca cgggcggcac ggtgtccttc aggacataga aagcgtccac gaccagatcg
     5521 gacgacccgg ccacgtcgaa aacgcggacg gtcacgtagt tcgtcgggcc gttcgggagg
     5581 gcgtcgaaaa cggcgttggg aagggaccag tcggcggtgt aggtggcggc gttgatggag
     5641 gcgacgattg tggtgaagtc cacgaggaag gggccgacgt cgccggacat cgtggacacc
     5701 cggatttcaa atcgcgccag ctggcttccc ccggaatcga ggaagttgac gtcgtagagg
     5761 cccgtgttgg cgatgcgcca gaagtcgtcg cccccctgcg cgttggagat cgtcggtggc
     5821 gttgtgtcct tgaggatcgt gaacacgtcg gtggagactt tgacgaactc cacccggtcg
     5881 tgggccctca cggagacata gttggtccct tccgttaggg aggagaacgc cgccggccag
     5941 tccgtgtcgt agtcaggtgc gccgtccagc ccggaggcga tggtcgtcca cggcagtacg
     6001 tcgcccactc cgccggacag gccggccgcc gaggagaccg tatactgcac cgtggttagt
     6061 ccggaggcca gatcgtggaa gtccacgtcg tacagggtcc cttccgcgcg ccgccagacg
     6121 gcgtccccgg gttggttgtc ggtgatcgag ggcggggtcg tgtctttcag gacgaagaag
     6181 acgtcggagg cgctgcccga ggaacccgaa acgtccacca cgcgcacgct gatgtagttg
     6241 gtcccctcct ccaagaggtc ccacgtgccg cccggcagcg cccaggggtc ggtgtattcg
     6301 gccgctgaga tgccgaaaac cgccgtggtc cagtcttgga tcagggattg ttgaatcggc
     6361 gccctcgcgg cgcgcgtctg gaaataatcc agagcatagg cgtcgctgtt ggaactgaag
     6421 gccacgttgt aaagggtccc gtcctgtcgt ctccagacga aatccccggc ctggttgtcg
     6481 atgatgttcg gcgggccggt gcccttgaac acgtagaaga cgtcgctttg cgtcacgctg
     6541 ccgccggcgt gatcgaaagc ccgcacggac acataatttt tcccgagttg cagggcgccg
     6601 aaaccgatgc cccattcctg atcgtaatag gtggcgttga cggtgagcgc gatttccgtc
     6661 caatctttga tgagcgtccc gctttgaccg gcttccgaaa agacgcggaa ttgcaggttt
     6721 ttcaacaggg atccgccggc gtcgttgaag tccacgtcga acaccgcgcc ggggttggcg
     6781 tagtaggtgg tgtcgtcccc catctggttg tcggtgatcg tcggcgccgt ggtgtccttg
     6841 aagaccagga acgcgtccgt cagagagctg acgttgccgg cgttgtcctc cacgcgcagg
     6901 ctgacgtagt tggtggctcc ctccagaaga gcgtcaaagg ccgccgagga gaggggccaa
     6961 tcgtcggcat aggcggccag ggcggccgag tcgaagatcg gggtgagcgg aactcgttcc
     7021 gcccccgtct gtccgggccc ggtgaagacc cggtactggg ccgagttcag cagggaggcg
     7081 tcgtcctgaa aatccacgtc atagatcgtg ccggaagaat ttctccaatt gaaatccccc
     7141 gcctggttgt cggagatcgt cgggaagaca ttgtcctttt tcacatagaa gacgtcttcc
     7201 gcggtcgtct ctgccccggc caggttgtag acgcgcaccg agacgtaatt gacgccctgg
     7261 gccaggccgt tgaagttgac ggtccagttc gtctcgtagg cggccgtccc gggggaagtg
     7321 aaaatggccg tccagggatt ttttcggtgc cggtgagagc cggcccggtc cagaccgtat
     7381 actgcgcctg cgtgagcagc gaatccgggt cgtggaagtc cacgtcgtag gcggtgccgc
     7441 cgaaccggcg ccaggcgtcg tcgccggact gcgcgtcggc gatctgcggg ggactggtgt
     7501 ctttgaggat atagaatccg tcggtcagaa cggcgccggc gttgccggcg gcatcgaagg
     7561 cccgcaagga gatgtaattc tttccgggcg gaagggtctc gaagacgccc gaaggcagcg
     7621 accagttcgc cgtgtaatcc gcggcgccga ggccggtggc cacgtccgac cagtccgggg
     7681 cgaacggccc ggtccctccg gcgacggtgc tggcccgtat ctggaatttt tccagcagac
     7741 tgccgccggg gtcgtcatgg aagtcgacgt cgtacgagcc ggtgttggcg gtgcgccagg
     7801 tgtcgtcgtc cggttggttg tccacgaggg tcggcgcctg ggtgtctttc ctcacgtaga
     7861 aaacatcggt gaaggtgcgg tcgttcccgg ccttgtccca gacatgaacg gacacatggt
     7921 tctggccttg ggacagagcg gcgaagtcga tgccccagtc ggtcgtgtac tccgttgaac
     7981 cgtggttcgg gccgaaaagg gtggtccaag gaatgatttc cgtcccgcct cctcccgaca
     8041 gcgaggtgac gcggtattcg gcccggtcca aacgcgacac caggtccctg aaatcgacgt
     8101 cgtagatcgc ccccggatcg gcggtccgcc aggtgtcgtc tccggtttgt tggtcgatga
     8161 ctgacgggag ggagacgtct ttgttgacgt agaaaacgtc gttcaacgtt gtggtggtgc
     8221 cggcggtgtc ccacacgcgc acggacacgt agttggtggt ctcgccctct tccagggcgg
     8281 agaagtccac ttgccagtcc gtttcatagg cggctagatg gagcccgacg aaaatcgggg
     8341 tccattcctt gcgcacggtc ccggtttggt tgggtcccag ggtgatgcgg tattcggcgg
     8401 tgtccaggag cgctccgccg gcatcggcaa agtccacgtt gaacgaggcg ccgggcgcgt
     8461 tcagaaaggc ggccacgtcg gcttgggaat cctgaatcac cggggcgatg gtgtcgacga
     8521 ggatctcgta ggagggcgaa gaataatccc cggcgttccc ggccttgtcc acggcgcgca
     8581 cgcgccaata atacgtggcg gtcggcaaat ccgccgccgg tgtggcctgg gaaaccgcgg
     8641 gcgaggggct gtaggagagc gttccgaacg cggggtccgt ggagacctgc agttcgtaat
     8701 ggtccacccc ggaggggacg tccggcgcat cggaccagtc aaaggcggga cgggggtctt
     8761 tcgtgacgct cccgtcggcg ggagagagcg gcgacggcgt cgccggcggc accgtgtctt
     8821 taaggacgaa gaagacgtcg gtcgattcgg cggagatgtt ccccacggtg tcgaaggccc
     8881 gcaccgtgat gtaattggtg gcgtgggaga ccagggcgtt gaagaagagg accggcaggg
     8941 cccaatccat ggaataggag tcggcgttga tgtcggagac caccgtcgtg taaccaacga
     9001 gggaaggttc cattccaccg ggaagagtgg aggccttcac ttcgaaacga gccaaccggc
     9061 tcccgcccgt gtccgcaaaa tccacgttgt acacggcccc cgggttggcg ctccgccaga
     9121 ccgtgtcgtc gccgggaacg gggtccgtga cggcggggct ggcgcggtcc acccggaacg
     9181 agaaggtgga ggtgtcggcg gaatagttgc cggcgttgtc gcgcgcccgc acgcgccagt
     9241 aatagagccc cgtcgacaag ctcgtggtcg aggcttggga gacgatcggc gaggagctga
     9301 aactcaaaat tccgaaatcg tcccgcgtcg acacctccag gacgtaatcg gccggcccgg
     9361 aggcgccgtc ggagacatcg gaccagtcga aagagatgtt gccgtggttg ttttggacgt
     9421 tgtccgcggg ggacagcagc gtcggcgtcg gcgggctcgt ggtgtctttt cgaacgtgga
     9481 acacgtcggc ataggtggag gtgttggtgg aaagatccac ggcccggacc gatacgtagt
     9541 tcgtcccctc ccggcaggcg gcaaaatcca ccggccagtc ggcggtgtag tgggtggcgc
     9601 cgatccccaa agcgatggcg gtccaatctt tgaggagcgt tccggattgg tccggggcgg
     9661 agaagatacg gtattgggcg taactgaggc tggagccgcc cagatcgtag aaatccacgt
     9721 cgtaggtcgc gccggcggcg ttgcgccagg cgtcgtcgcc cgcctgcagg tcgctcaccg
     9781 tcggcgtggt caggtccacg cgcaaggtgc gcagcacggc gttgctcgaa gagttcccgg
     9841 cgttgtcccg ggcgcggacg tgccaataat agaaaccttg cgccagcgtg gcggtgctgg
     9901 cctgggagag ggcggtcgac gaactgaaga cgatggatcc ggcggtgaag gccgggtcgg
     9961 tggccacctc caggacataa tcggcgacga gcgaggaggc gtcggtcgag tccgtccaat
    10021 cgaagaggat gttcccggag ttgaggttga cgttgtccgc cggagaagag agcagcggga
    10081 cggagggcgg cgtggtgtct tttcggacat aaaaaacgtc gacagcggca gcggtgtggg
    10141 tggagaagtc cacggcccgc accgagacgt agtttcccga ggtttcctgg agggcggcaa
    10201 aatccaccgg ccagtcggcg gtgtaatagg tggcgccgat ccccagggcg agggtggtcc
    10261 aatctttgag gagcgtgccg gtctggccgg gtccggagaa aacgcggtat tggacgtagc
    10321 tgaggctgga tccgccgaga tcgtagaaat ccacgtcgta ggtcgtgccg gcggcgctgc
    10381 gccagatatc gtctccggat tggaggtcgc tcaccgtcgg cgtggtcagg tccacacgga
    10441 aggttttcgg ggaggaatag tcgccggtgt tgccggccac gtccacggtg cgcccgcgcc
    10501 aatagtagaa atcttgggac aggtccgtta ccgatgcctg cgagtccgtg ggggaggagc
    10561 tgaagctgag gacgccgaaa ttgatatcgg tggaaacctg gacgaaatag ttctgcaccc
    10621 ccgagccggc cccgtccgag acgttttcga aatccaaaac cagggtgccg gtgctgaggc
    10681 gggcgtcctg gatcggcgaa atggccgtca tggccggcgc cggcgccgag gtgaggtcga
    10741 tgccgacgtc cgacgcggat tcggcgttcc ctccatagct gacccacgcc ccgttgcctc
    10801 ccgtgccttg cacgcgcgcc tgccagtgat aggtggtgtt ggtggagagg ccgctcacgg
    10861 tgacggagcc ggtcagcacg gaaccggaat acgcctgcgc gacgccggtc tgcgtgcccg
    10921 tgccgtcaaa ggcctgcccc acgggcttga tctcgatctc cggcgtcagg gtgtcggcca
    10981 ggggcggcgc gtccatggag aatttgaaca ccagggttcc gtcgttgatc cactgtccgg
    11041 tggagacgac ggtggttccg tcggatttat acggggtgag ccccgtgggc gggcggggcg
    11101 gcggaaaata ggtgtagctc accaccgcct tgttggagaa aacggcccga tcggtggagg
    11161 tgacgtcgtg gttcccggtg taagtcaccg cgttggtggg ggtgacctgg tttgtgctgg
    11221 tgtaaaggac cgtggcggtg aaggcttcgg ccggatgttg gtggtccacg gtttgtgaat
    11281 tggtgccgcc cacgttgatg agggtgtccc ggtcggcgcc cgcggcctgg gagttgttga
    11341 accggtcttg cagatacgcc gagcggagcg tcttggtccc gagaggggtg gtggccgtcg
    11401 ggaaatagac ggtgaagttc tgggcctgcg atgtggtggt ctgctggctc gactggccgg
    11461 cgaagacgga gtaggactcg gtgaacgccc cctcgttggt gtagtcgtag gtggcgatga
    11521 gttccacccc ggttccgccc cggcccgttt gggcgggata actcaaggtc acggaggacc
    11581 cgttcaccca gctcccggcc tgttccgtga ggtcgtgaag gaaagagtat ccggagatcg
    11641 tcgccgcctg tcccagcgtg ctgtagctcc gttgggtcac gctctgaccg gccacggcgg
    11701 aatcaaggac aaagttggtg agcgtggcca cgtcgaaggt gctgctgatc cgggcgtaga
    11761 ggcgtttgag ggtgatgttg ccttccagaa ggaaaaggtc ctgcaaaata ttccccgcgg
    11821 cggcggcccc cggggactgg ccgaggaaat aggcgacggt cttcgtcttg gtggccgagt
    11881 tgccgttgaa cgtataggtg acgacgcatt ccccgttcaa caccgtgatg ttggcgttcc
    11941 cgccggagac gatgttcagc gtctgcgagg tgttggcggc gaacccgggg accgaggtct
    12001 gcgcctgcgt gaggtagagg aaatcgtagg tgacggtgcc cgctccctcg aagaacagga
    12061 cgggttcggt ggtgtcggag ccgatgcggc agcgcaggtt gccgtcggcg gtgctgctgt
    12121 tggactggcg ctgtccgcgg atttcgaacc attgctgctg gacggcgacg ccgctgtcgg
    12181 cgatctcggc gttgtaggtg aaggcggtgg tggacccgcc ggtgagttga gactgcgccg
    12241 aggtcacgtt ggaggcgtag taggccggga agcgcacggt cttcacctgc gtggcggagg
    12301 tgtcgtcgta ttcgtaggtg acgtagagtt tcagggcgtg catgttgctc gacggtccgg
    12361 tgagggtgac cgaggcggaa taagtctggt tggaccaggc ggtcacctgc gcggtggcgt
    12421 ccgccttggc cgccacgcgg aacagttcgc cggtctgagt gatgtaggtg ccggcgccat
    12481 cgaagaccag cgtggacgcc gtgcccccgg tgttgaaacg gatctgcagg tccgacaggg
    12541 tggtgctcac gctggccagc gcctcgagtt ccaggtaggc gttgcggatg accaccccgc
    12601 tttccggcag gtgcacggtg atggtgggcg gcgtccaggc ggtgccggaa cctgcctcgg
    12661 tcgcgctgat cgcgctcggc ctcgccggac tgggtctgcg ccgcagacgc ggctgaaaag
    12721 agcccggtag ctcatccacc cgacaacacg gccccggcgc tcctcgcccg gggccttctc
    12781 acttcgaggc gccggctgcg caatgcaccg ccagccacag cgcatcggcg gagctcgact
    12841 cgacacggtg gcggcaatgc gccgggatcg tgatccagtc gcccgccttc agcttcacgt
    12901 cgtcaccgcc gtcgaagcac agcaccgccg cgcccgccag cagcaccacc cactcgtcgc
    12961 cgtgctggtc gtaccagtcg tcgggcgcgc tggcgtggcc gtgcgagacg atgcgctcga
    13021 cacggcagcc cggattggcg aacaaggtct gga
//
LOCUS       DAOTFD010000055        12420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3572_length_12420_cov_3.955924, whole genome shotgun sequence.
ACCESSION   DAOTFD010000055 DAOTFD010000000
VERSION     DAOTFD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_12420_cov_3.955924"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="P5079_04920"
     CDS             complement(<1..1311)
                     /locus_tag="P5079_04920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY29364.1"
                     /translation="MSTSSLAERLKSPDKTARRLACEELDAREGLAHVGALIHLLEDS
                     DAAVHEAASQALKRIGGERVVRALLPVLSSDNESARNTACEILETVGSQALDPIVETL
                     RHEDADVRKFVADVLGNLGDARAVPALMISLGDKHPNVVTSVIESLGKLKDVRAVDPL
                     SKQLRGEFVFESAFALGSIGDARALAPLLEIAGSRSSVARYAAIEAIGLLGKKEAIPA
                     LLEYLREGNPIVQQAVLKAVGEISPHYLEEEIQGHRELWKVDGLCRSLDSEDLVLRRY
                     ALIALGTLNDGKGNRKLVEFLHSADEEDVSFAVNSLIRIGQKDIAPLLPALSSSNANV
                     RRYVALALGDVQNGSASPALSAAAKDADPLVRAAAVRSLGKIADSAMAETIVQSLDDE
                     EELVRKAACDALGWLGEESVLEPLWRSLKDESVEVAESALGALVM"
     gene            complement(1317..1691)
                     /locus_tag="P5079_04925"
     CDS             complement(1317..1691)
                     /locus_tag="P5079_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY29365.1"
                     /translation="MSPQKILLVEDDDYILDFINFALQRTGYEITTVGDGAAAMEAVM
                     KDPPDLIVMDCILPKMSGLQLCTELKSNSKSQSIPIILMSASLRIKPEEIVETYGVEC
                     FLRKPFHSVELLSAIDSVLKKG"
     gene            complement(1728..2792)
                     /locus_tag="P5079_04930"
     CDS             complement(1728..2792)
                     /locus_tag="P5079_04930"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRY29366.1"
                     /translation="METEEKIRVLVVDDSAFMRSAIKGMLQIDPQLQVIATARDGEEA
                     IEKIRQYKPDIVTMDIEMPRMDGLTATRIIMKEMPVPILIVSSLSEEGAQVTFDALEA
                     GAVDFISKDLGNRSLNILNIEQDIIAKIKTIFHRKKDFGRFMERFRQSQIVPVDTRLA
                     PLGGGNVLLVAIGVSTGGPKALHDVIPYLPKGLSAAVAVVQHMPAAFTKSFADRLNDI
                     SALEVQEAQDGLVVRPGMALVAPGGRHMRFRRRNLEVTVEISDIPRDSLHKPSANEMM
                     LSAVKTYGASRVLGVIMTGMGNDGLLGMRAIKSGGGKTIAQDEASCVVYGMPKAVVDE
                     GLANKVVPLEQLANEIVNMV"
     gene            complement(2818..3747)
                     /locus_tag="P5079_04935"
     CDS             complement(2818..3747)
                     /locus_tag="P5079_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRY29367.1"
                     /translation="MPDVRAGNYLAIRNSLEEKTGIPFTCYNEDFLQRHIAARLESLG
                     MSAEKYRSFIDENAEEAERFSECLTVNYTGFFRDAGKYKALRENVLGEIVRNNMRRKT
                     LSLWSAGCAAGEEPYSVVMSLMEGLGVERAGWRMDMLATDVNGALLERARSGVYPASQ
                     VLKSRLASTIFRKYFESLEQTSDGAGVPSFRVAGEVRERVQFRRHNLLRDPFPTAVDL
                     ILCRNVMIYMKGEHVNVLVEKFHEALKDGGYLVLGNYEALDHTFIPLFERLKVSGEYF
                     YRKIPTGSVLQKAMSQRKTLIVDSLSRVQLKDQ"
     gene            complement(3704..4465)
                     /locus_tag="P5079_04940"
     CDS             complement(3704..4465)
                     /locus_tag="P5079_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29368.1"
                     /translation="MERADDENRVLVVDDDARIGQLIEDVLGRDGYRVGCARSGEEAF
                     RAYRENPDQLVLLDMVLPGESGLEILKGLKKINPEARVVVMTGYSVLGMLENASHLGC
                     LAALIKPFTIDDLRQTVVSTTGKKAVEESEHIYSALVIDHEDRYAQLLGKAWRDLDFL
                     VVKYPSIAEARKHDRGMDFDLILVNKDTVQRASEGERKYLDEKTPTVKLLLRLDTGAS
                     VENIVKNIETIFPKKTGRKAKNAGRPGRELPGHQK"
     gene            complement(4484..5302)
                     /locus_tag="P5079_04945"
     CDS             complement(4484..5302)
                     /locus_tag="P5079_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRY29369.1"
                     /translation="MEQKWEVFNDIKAYLKGRGLHCDYYRDAYLERRLVVRLKKCGKT
                     SFADYLSHLRSDPAECQKLTDCLLVNVSSFFRDGTPFENLQRNIFPRLSRRAAQEGRR
                     ALRVWSAACATGEEPYSIAMSAHEAIPDFFARGDKLKIVASDIDEESLKSARRGEYPA
                     KTMQGVEDRLLRKYFSRKGASYAVMDFIRESVHFVKNDLIGDMPPWQGLDLILCRNML
                     IYIHLRMQEHILGKLWGALGAGGVLILGKSEVLPGSLAEKFEEVSPQDGIYFKR"
     gene            complement(5309..5701)
                     /locus_tag="P5079_04950"
     CDS             complement(5309..5701)
                     /locus_tag="P5079_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY29370.1"
                     /translation="MDEKRKFKRVPGKFVVQYSELSLSEKARDVFLSQLVNISASGLL
                     FESAQAFAVDTVLKLELKMDRWQKFSSDFYKYDQTSVSEPFVAVGKVVRVESMGKGKY
                     LIGVFLTSVYESHRDALKKYIESLSEAK"
     gene            complement(5783..6271)
                     /locus_tag="P5079_04955"
     CDS             complement(5783..6271)
                     /locus_tag="P5079_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY29371.1"
                     /translation="MDATLTEKKEGGKLLQLVSFKLGKGEFGIDILMVQEINRMLDIT
                     RVPKSPDFIDGVINLRGRVIPILNLRKRFGLPEKEHDKSTRIVVVNVNNKILGMIVDA
                     VSEVLRLPSSTVEPPPPMVSGIESEYIKGVGKIDEKLLILLDLNKLLTSKEQQMLEHV
                     AK"
     gene            complement(6312..8078)
                     /locus_tag="P5079_04960"
     CDS             complement(6312..8078)
                     /locus_tag="P5079_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY29372.1"
                     /translation="MRSFNDMKMFTKIFVLVAVVITVIALPIFTLVMPRVKAQIREDK
                     EAQLRYLTQSVYTAIEGHYKAYQAGLMSEEGAKKAVVNLIKMIRYEGDNYFWINDSFP
                     RMIVHPIKPELDGKDQTDYKDPNGKQLFVEFAKVCKQSGEGFVDYEWPKPGFSKPQPK
                     ISYVKLFKEWDWIVGTGVYVDDMKAVIAKLQWYVVGIVVGGLLIALLVSYIVGRKVVQ
                     PILKMVDMIKDVAEGEGDLSKRLELDSKDELGELAKWFNVFIEKLHDIIAQIANTTVQ
                     AAAAGEQASASSEELASGAEEQQAQTTQVATSVEEMSATIQEVAKNANEAANSSKSSG
                     EAAKKGGEIVQKTVEGIMRISDSSKATAEAIKVLTKNSEQIGSIIGVIDDVADQTNLL
                     ALNAAIEAARAGEQGRGFAVVADEVRKLAERTSSSTKEITGMVKTMQEGTQMASQAME
                     RGMKDVDGGVKLAEQAGNTLKEIVVTAQHVVDLVQQVATAAEEQSAAAEEISSNVESV
                     ASVAKQTAVGTQQISEASQEVAKLADGLQSLVNKFKLKAGSVKQAERREHVKHDYKVS
                     SSAPAHAVPAPKKPVVAHEKKA"
     gene            complement(8158..9309)
                     /locus_tag="P5079_04965"
     CDS             complement(8158..9309)
                     /locus_tag="P5079_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY29373.1"
                     /translation="MKTTWKAVWVLALFLLASSVCPVFSYEIDAEQNKRIKDLEEKVS
                     ALKGGGDPESAKKEQEIQALKKEVADIRQALEASKSAQTQGKFLNTTYGTLTMKGIVQ
                     TQFDQGQHGTPSGTFLVKRARLIFSGLAPVQKVGYFIQTDATGEIFLLDAKVMFNQIP
                     KTTVNVGRFVPAFTYYMPRTVADLDFINYPLLVTKYAPWRQVGAQIDTKFKYANFNAG
                     IFNGYPANGWQDNNPAKDYLGRMEITPFKDFGIFGYRWEGREKPTTGPDAEKNRTGGG
                     LSLRYKLVNVVGEYVRGYDESSTKKDSSGYYVNAGVKVLPFWELLCRYDWFDVDLNKP
                     LDAEDWVTLGTNYFLDGNNLKFSVNYIFKREEGTPIDNDEVLAQMQVFF"
     gene            10021..11517
                     /locus_tag="P5079_04970"
     CDS             10021..11517
                     /locus_tag="P5079_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRY29374.1"
                     /translation="MISTYGRIKNSFVALALTASVGICILAFPLRAAQSGVETKIALE
                     ESLERRLKSVISQILGNDNVIVIVTAEMLTEEEKKKVETTPEETSVLPGVPLKEKIGE
                     RDDISALAIKESRTLIKKLAATVVLDVGVSKTDENVIKNVTTGLLGLKTDRGDSLEIQ
                     RMDFHRGEKGFNWRRFITPPDLWWTMGLALMAFFILFITAFFFGSFRVFIREFVKALR
                     SYAESMEEQIKMIHMQKDREKELKGRAAELEEAAVPEPLLASPQKANGNGLSPAEENG
                     KDSAPFSFLNGSHLRNLKILLKKENPDQIAIVVNYLPPSLANDVLASLDAAQQAEVMA
                     QLSRVEELDPDLVKAVESKIKEKIAFVIGGEDRLQSFVDNADPLTQQSLLATIAKKDA
                     PLSARLRNRLFTLEDLAKLEGPVLAVIVRRINTRALAGVLRELPKELQAKILSSLPAG
                     SSAMLKEEIELSKPLPPQRLAQEQKRILDAIRRLNDEGVIQLRKGAAS"
     gene            11517..12317
                     /locus_tag="P5079_04975"
     CDS             11517..12317
                     /locus_tag="P5079_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY29375.1"
                     /translation="MDLMTVLGVLSGAGAIYYVLHHGGMLHLIVNLEAFILIFGGTLG
                     SVLISYPWEALKTVPRALIMVLVPPRKITFAQLIYTFVQMAEKARINGLDSLQTDAAG
                     KHFFLRDGVQMLLDGLEPEIVKERLEREILLTRQRHMQVTGIFRSAGTFAPIFGLLGT
                     LIGVVQVLRNLTDPHSMGASMAIAMTASFYGIFAANFLFLPIASKLNYHSENELLVKE
                     LIAKGIMALQRGDIPLVVSKKLEAYLSYRLRTKQHSRTAAGAKPRRAA"
BASE COUNT         2382 a         4151 c         3189 g         2698 t
ORIGIN      
        1 catcaccagg gcgccgagag ccgattccgc cacctcgacg gactcgtcct tcagggaccg
       61 ccacaacggc tcaaggaccg attcctcacc caaccacccc aaggcgtcac aggccgcctt
      121 ccgtaccaac tcctcctcat cgtcgagcga ttgaacgatc gtctccgcca tggcggaatc
      181 cgcgattttg cccagggagc gcacggccgc cgcccggacg agcggatccg cgtctttggc
      241 ggcagccgac agggccgggg acgccgaccc attctgcacg tccccgagcg ccagagcgac
      301 atatcggcgc acgttggcgt tggacgacga caacgccggc aacagcgggg cgatgtcctt
      361 ttggccgatc cggatcaaac tgttcacggc gaacgacacg tcctcctcgt ccgccgaatg
      421 caggaactcc accaactttc tgttgccctt gccgtcgttc aaagtcccca acgcgatgag
      481 ggcataccgc cgcaacacca ggtcctcact gtccagcgac cgacaaagac cgtccacctt
      541 ccacagttcc cggtggccct ggatctcctc ctcgagatag tgcggagaaa tctcccccac
      601 ggccttcagc acggcctgtt gaacgatcgg gttcccctcc cgcagatatt ccaacagcgc
      661 ggggatcgcc tctttctttc cgagcaaccc gatggcctca atggcggcgt agcgcgccac
      721 cgacgaccgg ctgccggcga tctccaacaa cggggccagc gcccgggcgt ccccgatgct
      781 ccccagcgcg aaggcgctct caaagacgaa ctcgccgcgc agctgtttcg aaagaggatc
      841 caccgcccga acgtccttga gttttcccag actttctatg acggaggtga cgacgttggg
      901 gtgcttgtcc cccagactga tcatcagcgc cggaacggcc cgcgcgtccc ccaggttccc
      961 gaggacgtcg gcgacgaact tccgcacatc cgcgtcctca tgccgcaggg tctcaacgat
     1021 ggggtccagc gcctggctcc cgaccgtctc cagtatctca caagccgtgt tccgcgccga
     1081 ctcgttgtcg ctcgagagca ccggcaacag ggcccggacc acccgttcgc caccgatcct
     1141 tttcagggcc tgcgaggcgg cctcatgcac cgccgcgtcg gaatcctcca gcaggtgtat
     1201 caacgccccc acatgcgcca ggccttcgcg ggcgtcgagt tcctcgcagg caagacggcg
     1261 cgccgtcttg tccgggctct tcaggcgttc cgccaacgac gaagtggaca tggcccctac
     1321 cccttcttca gaaccgaatc gatggccgac aacaattcga ccgaatgaaa cggcttcctc
     1381 aaaaagcatt ccaccccgta ggtttcgacg atctcttcgg gtttgatccg gagggaggcg
     1441 ctcatgagga tgatggggat cgactggctc ttggaattgc tcttgagttc ggtgcacaac
     1501 tgcaaaccgc tcattttggg cagtatgcag tccatgacga tgaggtccgg agggtctttc
     1561 atcaccgcct ccatggcggc ggcgccgtcg ccgacggtgg tgatctcata gccggtccgc
     1621 tgcaacgcga agttgatgaa atcgaggata taatcgtcat cctcgaccaa aaggatcttt
     1681 tgaggactca taaccccctc cgtttatccg acgtgcgccg agcgccgcta caccatgttc
     1741 acgatttcgt tcgccaactg ctccaacggc acgaccttgt tcgccaaccc ctcgtccacc
     1801 acggctttgg gcatcccgta gaccacgcaa ctggcctcgt cctgggcgat cgtttttccg
     1861 cccccgctct tgatggcccg catcccgagg agcccgtcgt tccccatccc ggtcatgatg
     1921 acccccaaca cccgggacgc gccgtacgtc ttgacggccg aaagcatcat ctcgttggcc
     1981 gacggcttgt gaagggagtc ccgcgggatg tcgctgatct ccacggtcac ctccagattg
     2041 cgccggcgga acctcatgtg ccgcccgccc ggcgccacca gcgccatccc cggccgaacg
     2101 acgagcccgt cctgggcctc ctggacctcc aacgcgctga tgtcgttcag ccggtccgcg
     2161 aaacttttcg tgaacgccgc cggcatgtgc tgcaccaccg ccaccgccgc cgacaatcct
     2221 ttcgggagat aggggatcac gtcgtgcaag gccttcggcc cgcccgtgga caccccgatg
     2281 gccaccagaa gcacgttgcc tcctcccaac ggcgccagcc tggtatcgac cggaacgatc
     2341 tggctctgcc ggaaacgctc cataaaccgg ccgaaatcct tcttccggtg gaagatggtc
     2401 ttgatcttcg cgatgatgtc ctgttcgatg ttgaggatgt tcaacgatcg attgcccagg
     2461 tccttgctga tgaaatccac cgcccccgcc tccagggcgt cgaacgtcac ctgggcgccc
     2521 tcctcggaga gggagctgac gatcaggata gggaccggca tttctttcat gatgatccgg
     2581 gtcgccgtga gaccgtccat ccggggcatt tcaatgtcca tcgtgacgat gtccggctta
     2641 tactgccgga tcttctcgat ggcctcttcg ccgtcccggg ccgtcgcgat cacctggagt
     2701 tgcgggtcga tctgcaacat ccccttgatc gcgctccgca tgaaagcgga gtcgtccacc
     2761 accagaaccc ttatcttttc ctctgtctcc atcgtttctc ccgccgcccc gaagggccta
     2821 ttggtctttc aactgcaccc tgctgagact gtccacgatc agcgttttcc gctgggacat
     2881 cgccttctgc aaaaccgaac cggtcggaat cttcctgtaa aaatattctc ccgacacctt
     2941 cagcctctcg aaaagaggga tgaacgtgtg gtcgagcgcc tcgtaattcc ccaggaccag
     3001 atagcccccg tctttcaacg cctcgtggaa cttctccacc aacacgttca catgctcgcc
     3061 tttcatgtag atcatcacgt tccggcacag gatcaaatcc acggccgtcg ggaaagggtc
     3121 ccggaggagg ttgtgcctcc ggaactggac ccgttcccgc acctcccccg ccactcgaaa
     3181 ggaagggacc cccgcgccgt ccgaagtctg ttccagcgac tcaaaatact tgcggaagat
     3241 cgtgctcgcc agccggcttt tcaacacctg cgatgcggga tacacgccgg aacgcgcccg
     3301 ttccaagagc gccccgttca catccgtcgc aagcatgtcc atccgccagc ccgcgcgttc
     3361 gacgcccaac ccctccatca acgacataac gacggaatag ggctcctccc cggcggcgca
     3421 gccggcgctc cacagggaca gggtttttcg tcgcatgttg ttccggacga tctccccgag
     3481 gacgttctcc ctcaacgcct tatacttccc cgcgtccctg aaaaaacccg tatagttgac
     3541 ggtcagacat tcgctgaacc gttccgcctc ctccgcgttt tcgtcgatga aagaacgata
     3601 tttctccgcg gacatgccca gagactccag ccgcgccgcg atatgtctct ggaggaaatc
     3661 ctcgttgtaa caggtgaacg ggatccccgt tttttcttcc aggctatttc tgatggccag
     3721 gtagttcccg gcccggacgt ccggcatttt tggccttcct tcccgttttt ttagggaata
     3781 tggtctcgat gtttttgacg atgttctcca cgctcgcgcc ggtgtccaag cggagcaaga
     3841 gcttgacggt gggggtcttc tcgtccaaat acttcctctc cccttccgaa gcgcgctgaa
     3901 cggtgtcctt gttcaccagg atcagatcga aatccatgcc ccggtcatgc ttccgggcct
     3961 cggcgatcga cgggtatttg acgacgagga aatcgaggtc cctccaggcc ttccccagca
     4021 attgcgcata acggtcttcg tggtcgatga caagcgccga gtagatgtgc tccgattctt
     4081 cgacggcttt cttgcccgtc gttgaaacaa cggtctgacg aaggtcgtct atcgtaaacg
     4141 gcttgatcag cgccgccagg cagccgagat gggaggcgtt ttccagcatc cccaagacgg
     4201 aataccccgt catcacgacc acccgggcct ccggatttat tttcttaagc cctttcaata
     4261 tctccaggcc gctctcgccg ggcaacacca tgtccaggag caccagttgg tccggatttt
     4321 cccggtaggc gcggaaggcc tcctctccgg aacgggcgca ccccacccga tacccgtcgc
     4381 gccccaacac gtcctcgatc agctggccga tccgggcatc atcgtcaacg accaatacgc
     4441 ggttttcatc gtccgctcgt tccatggcac agcgcctcct cactcaacgt ttgaaataaa
     4501 ttccgtcctg cggcgagacc tcttcaaatt tctccgcgag cgagccgggc agcacctcgc
     4561 tcttccccag gatgaggact cctcccgccc ccaacgcgcc ccacagcttt ccgaggatgt
     4621 gctcctgcat cctcagatgg atatagatca gcatgttgcg gcacaggatc aggtccaggc
     4681 cctgccacgg aggcatgtct ccgatcagat cgtttttaac gaaatggacg ctctcccgga
     4741 tgaaatccat caccgcgtag gaggcccctt tccgtgagaa atatttccgc aagagccgat
     4801 cctccacccc ctgcatggtc ttggccggat actccccccg ccgggcgctt ttcagggatt
     4861 cctcgtcgat atcgctcgcc acgatcttca gcttgtctcc gcgggcaaag aaatccggga
     4921 tggcctcatg ggccgacatg gcgatagaat acggctcctc ccccgtggcg caggccgcgc
     4981 tccacacccg gagggcgcgc cgcccctcct gggccgcccg ccgggagagt ctcgggaata
     5041 tgttccgttg aaggttttcg aagggggttc cgtccctgaa aaaggaagag acgttgacca
     5101 agaggcaatc ggtcaacttc tggcattcgg cgggatcgga ccgcaggtgg gacaggtagt
     5161 cggcgaagga cgtcttcccg catttcttaa gacggaccac cagccgtctt tcgagatagg
     5221 cgtccctgta ataatcgcag tgaaggcccc tccccttcag gtaggccttg atgtcattaa
     5281 aaacttccca tttctgttcc acgggggctc atttcgcttc cgacagagat tcgatgtatt
     5341 tcttcaaggc atcccgatgc gactcgtaaa cgctggtcag gaacaccccg atgagatact
     5401 tccccttccc catcgactcc acgcggacga ccttgcccac cgccacgaaa ggttccgaaa
     5461 cggaggtctg atcgtattta tagaaatcgc tgctgaactt ctgccagcgg tccatcttca
     5521 gctccagctt caacaccgtg tcgacggcaa aggcctgggc cgattcgaac aaaagtcccg
     5581 aggcggagat gttgacgagc tgggacagga acacgtcccg ggccttctcc gacaagctca
     5641 actcgctgta ttgcacgacg aatttcccag ggacccgctt aaatttccgc ttttcgtcca
     5701 tagtgtcctc cgacgcccgg cggccgtggg agcgggatcg ctcccacgcc ggaacgtttt
     5761 tttgactgcc gcccgaagcc gcttatttcg cgacgtgctc cagcatctgc tgttccttgc
     5821 tggtcaacag cttgttcagg tccagcagga tcaacagctt ctcgtcgatc ttccccaccc
     5881 ccttgatgta ttccgactcg atgcccgaca ccatgggagg aggcggctcc accgtgctcg
     5941 acggcaaccg cagcacctcg ctcaccgcgt ccacgatcat ccccaggatc ttgttgttca
     6001 cgttcaccac cacgatgcgg gtggatttgt cgtgttcctt ctccggcagc ccgaaacgct
     6061 tccgcaggtt caggatcggg atcacccggc cccgcaggtt gatgaccccg tcgatgaagt
     6121 ccggggactt gggcacgcgg gtgatgtcga gcatccggtt gatctcctgc accatgagga
     6181 tgtcgatccc gaactccccc ttccccagct tgaagctgac gagctgcagc agctttccgc
     6241 cctctttctt ttcagtcaat gtagcgtcca tatacgttcc tccctttcgc cgttggagat
     6301 gcgtccgctt ttcaggcttt cttctcgtgc gccaccaccg gcttcttcgg ggcggggacc
     6361 gcgtgggcgg gagcgctgga ggaaaccttg tagtcgtgct tcacgtgttc gcgtctctcc
     6421 gcctgtttga cgctcccggc tttcagtttg aacttgttga ccagcgactg gagcccgtcg
     6481 gccagcttgg ccacctcctg ggaggcctcg ctgatctgct gggtgcccac cgcggtctgt
     6541 ttggccaccg aggccaccga ctccacgttg gacgagatct cctccgccgc cgccgactgt
     6601 tcctccgcgg ccgtggccac ctgctgcacc aggtccacca cgtgctgcgc cgtcaccacg
     6661 atctccttca acgtgttccc ggcctgctcg gccagcttca cgccgccgtc cacgtccttc
     6721 atgccccgtt ccatggcctg ggacgccatc tgcgtccctt cctgcatggt cttcaccatg
     6781 ccggtgatct ccttggtgga cgaagacgtg cgttccgcca gttttcgcac ctcgtccgcc
     6841 accacggcga agccccggcc ctgttccccg gcccgcgccg cctcgatggc cgcgttcaag
     6901 gccagcaggt tcgtctggtc cgccacgtcg tcgatgaccc cgatgatgct gccgatctgt
     6961 tcggagttct tggtcagcac cttgatggcc tccgccgtgg ccttggagga atccgagatg
     7021 cgcatgatcc cctccaccgt cttctggacg atctctccgc cttttttggc ggcctcgccg
     7081 gaactcttgg aactgttggc ggcctcgttg gcgttcttgg ccacttcctg gatggtggcg
     7141 gacatctcct ccaccgaggt ggccacctgc gtggtctggg cctgttgttc ctccgccccg
     7201 ctggccagct cctcgctgga ggcggaggcc tgttccccgg ccgccgccgc ctggaccgtg
     7261 gtgttggcga tctgggcgat gatgtcgtgc agtttttcaa tgaacacgtt gaaccacttg
     7321 gcgagttccc ccaattcgtc cttgctgtcc aactccagcc gtttggaaag gtccccctcg
     7381 ccctcggcca catccttgat catatccacc atcttcagga tcggctgaac gactttccgc
     7441 cccacgatgt aggagacgag cagggcgatc aacaaaccgc ccacgacgat accgacgacg
     7501 taccactgca gcttggcgat gacggccttc atgtcgtcca cgtacacgcc cgttcccacg
     7561 atccagtccc attccttgaa caacttgacg taggagatct tgggctgggg tttggagaaa
     7621 cccggcttgg gccactcata atccacgaac ccctccccgg attgtttaca gaccttcgcg
     7681 aactcgacga aaagctgttt cccgttgggg tccttgtagt ccgtctggtc tttcccgtcc
     7741 agttccggtt tgatggggtg aacgatcatc ctcggaaaac tgtcgttgat ccagaaatag
     7801 ttgtccccct cataacgaat catcttgatg aggttgacga cggccttttt ggctccctct
     7861 tccgacatca gtccggcctg ataggccttg taatggccct cgatcgccgt atagaccgat
     7921 tgcgtcaagt aacgaagctg ggcctctttg tcctcccgga tctgcgcctt cacgcgaggc
     7981 atcaccaggg taaaaatcgg caacgcgatg accgtgatca caaccgccac aagaacgaat
     8041 atcttggtga acatcttcat gtcgttgaaa cttctcatat gagtttcctc ctttttctat
     8101 cttcctgccg tagtccgtta tcccgccacc tcggccgatc gagaccggcc cgctgcccta
     8161 gaagaacacc tgcatctgcg ccaacacctc gtcgttgtcg atcggcgttc cctcttccct
     8221 cttgaagatg tagttcaccg agaacttcag gttgttcccg tccaggaaat agttcgtccc
     8281 cagcgtgacc caatcctcgg cgtccaacgg cttgttcaag tccacatcga accagtcgta
     8341 ccggcacagg agttcccaga acggaaggac cttcaccccc gcgttgacgt agtagcccga
     8401 gctgtctttt ttcgtggaac tttcgtcata cccccggacg tattccccca ccacgttgac
     8461 caatttgtat cggagggaga ggccgccgcc ggttctgttc ttttcggcgt ccggccccgt
     8521 ggtcggtttt tcgcgccctt cccaacggta gccgaatatc ccgaaatcct tgaacggcgt
     8581 gatttccatc cgtcccagat agtccttggc ggggttgttg tcctgccagc cgttggccgg
     8641 gtacccgttg aagatgccgg cgttgaaatt cgcgtatttg aatttggtgt cgatctgcgc
     8701 gcccacctgg cgccatgggg cgtatttggt gaccagcaag ggatagttga tgaagtccag
     8761 gtcggccacg gtgcgcggca tataataggt aaaggccggg acgaaccggc ccacgttcac
     8821 ggtggttttt ggaatttggt tgaacatcac tttggcgtcc aacaggaaaa tctccccggt
     8881 ggcgtcggtc tggatgaaat acccgacttt ttgaacgggc gccaagcctg aaaaaataag
     8941 acgggccctt ttcacgagga acgtgccgga cggggtgccg tgctgcccct gatcgaattg
     9001 ggtctgaacg atccccttca tcgtcagcgt cccataagtc gtgttgagga atttcccctg
     9061 ggtctgggcg cttttggagg cctccaacgc ctgacggata tccgccactt ccttcttgag
     9121 ggcctgaatc tcctgttcct ttttggccga ctccggatcg cctcccccct tcaacgcgct
     9181 gaccttctcc tccaaatctt tgatccgctt gttttgttca gcgtcgatct catacgaaaa
     9241 aacgggacag accgaagagg ccaagagaaa cagcgccaga acccaaaccg ccttccatgt
     9301 cgttttcata ttttctcctt ctttcatctt cttaaccgca tctctgttcc ggccctcttc
     9361 cgccctcttc atttttccca tgtccttcag aggccctcga cttcttttgg atctcaaaaa
     9421 aacgtgaccc tcgattggac agcacaaaag cccttcgaaa aaatattcga ggggctttgt
     9481 cggaacgcaa ggaatgtcgt ctttgtcata tttagacttt caccttgaaa tgctcgctga
     9541 cttttcaacc ggttgacccc tgttctcaaa attgaaccgt tttatggtta tactgctttt
     9601 attgcttttg cattttaaac taacaatttt gtatttcacc acaactttgc accaaaaacg
     9661 tctccatgtc aagggggtgt gacaaaagtc acacagaatt ttttatgacg agtttgacaa
     9721 atttactcgt ttgactattt taattgtcta tttttgaggc aaattttata ttttcgctcg
     9781 ccttccgtcg acattatcag tcccctcaaa aatcgccgac atttcagccg acgttgcccg
     9841 tcatcgccac ccccccctcc ccctatttgt caaccacacg aaagcccaca gaatccaacg
     9901 attcctttta aaaaacgaac atctctccgt gttttttcaa aagcagtata ctcagcgaag
     9961 gcccaaacca cccccaagca ttgacaaacc gcgtgtattt cgtaaaatct accccgtccc
    10021 atgatttcaa cttatggccg catcaaaaac tcttttgtcg ccctcgcgct gaccgcctcc
    10081 gttggcattt gcattttggc gttccccctc cgagcggccc aatccggggt ggaaaccaag
    10141 atcgccctgg aagaaagtct cgaacgccgc ctcaaatccg tcatctccca gatcctcggc
    10201 aacgacaacg tcatcgtcat tgtgacggcg gaaatgctca cggaagagga aaagaaaaaa
    10261 gtggagacca cgccggaaga gacctccgtc ctgcccggcg tccccctgaa agagaagatc
    10321 ggggaacggg acgacatctc cgccttggcc atcaaggaaa gccgcaccct catcaaaaaa
    10381 ttggccgcca ccgtggttct ggacgtgggc gtgagcaaaa ccgacgaaaa cgtcatcaag
    10441 aacgtcacca ccggtctttt gggcttgaaa accgaccggg gcgattccct cgaaatccaa
    10501 cgcatggact tccatcgcgg cgaaaagggt ttcaattggc gccggttcat caccccgccc
    10561 gacctttggt ggaccatggg ccttgccctc atggcttttt tcatcctctt catcacggcg
    10621 ttcttcttcg gctctttccg cgttttcatc cgtgaattcg tgaaggccct acgcagctac
    10681 gcggaatcca tggaagaaca gatcaaaatg atccatatgc agaaagaccg ggaaaaggaa
    10741 ctgaaaggcc gtgccgccga actggaagag gccgccgtcc cggaaccgct gctcgcctcc
    10801 ccccagaaag ccaacgggaa cggcttgtcc ccggcggagg aaaacggaaa agattccgcc
    10861 cccttctctt tcctgaacgg ttcccacctc cgcaacctga agatcctcct gaaaaaggaa
    10921 aatccggatc aaatcgccat cgtggtgaac tatctgcctc cttccctggc caacgacgtg
    10981 ctggcctcgc tggacgcggc ccaacaggcc gaggtcatgg cccagctttc ccgggtggag
    11041 gagctggacc ccgacctggt gaaagcggtg gaatccaaga tcaaggaaaa gatcgccttc
    11101 gtcatcggcg gggaagaccg cctccagtct ttcgtggaca acgccgaccc gctgacccaa
    11161 cagtctcttc tggccaccat cgccaaaaaa gacgcccccc tgtccgcccg gctcaggaac
    11221 cgcctcttca cgctggagga tttggccaaa ctggagggcc ccgtgctggc ggtgatcgtg
    11281 cggcgcatca acacccgcgc gctcgccggc gtccttcggg aactccccaa ggaactgcag
    11341 gcgaagatcc tttcctccct gccggccggc agctctgcca tgctgaagga ggagattgaa
    11401 ctctccaaac ccctgccgcc gcagcgcctg gcgcaggaac agaaacgcat tttggacgcc
    11461 atccgccgcc tgaacgacga gggcgtcatc caactccgca aaggagccgc ttcctgatgg
    11521 acctgatgac cgtcctcggc gtgctgtcgg gcgccggggc catctactac gtcctgcacc
    11581 acggcggcat gctccacctc atcgtcaacc tcgaagcctt catcctcatc ttcggcggca
    11641 ccctgggctc ggtgctgatc tcctacccgt gggaagcgtt gaaaacggtc ccccgcgcgc
    11701 tgatcatggt gctcgtgccg ccgcgcaaga tcaccttcgc ccagttgatc tacaccttcg
    11761 tccagatggc ggaaaaggcc cggatcaacg gtctcgactc ccttcagacc gacgccgccg
    11821 ggaaacactt cttcctgcgc gacggcgtgc agatgctgct ggacgggctg gaaccggaga
    11881 tcgtgaagga gcggctggaa cgcgaaatcc tcctcacccg ccaacgccac atgcaggtga
    11941 ccggcatctt ccgctcggcg gggacgttcg cccccatctt cggcctcctg ggcaccctca
    12001 tcggcgtggt gcaggtcctc cggaacctca ccgacccgca ttccatgggc gcctccatgg
    12061 ccatcgccat gacggcttcc ttctacggca tctttgccgc caatttcctg ttcctcccca
    12121 tcgcctcaaa actcaactac cactccgaga atgaactcct ggtgaaagaa ctcatcgcca
    12181 aggggatcat ggcgctgcaa cggggagaca tccccctggt ggtctccaag aaactggagg
    12241 cgtacctgtc ctaccgcctc cgcaccaaac agcattcccg gaccgcggcg ggcgccaagc
    12301 cacgccgcgc ggcatgatcg gctccctcct ccgccacacc tccatctcgg ccaccacgga
    12361 agaaaacccg ctctggctgg tggtcctctg tgacatgatg accaacctca tgctcttctt
//
LOCUS       DAOTFD010000056        12418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3573_length_12418_cov_4.602200, whole genome shotgun sequence.
ACCESSION   DAOTFD010000056 DAOTFD010000000
VERSION     DAOTFD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12418
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_12418_cov_4.602200"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5079_04980"
     CDS             complement(<1..550)
                     /locus_tag="P5079_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29376.1"
                     /translation="MRITNLHLIFSFFFLSAAGFFAYRHFLPSWTSLPKAAPAVPALT
                     ADMLEQERRSAEERKQLAVARERAEQSLRYADLAIETAQKEERDATAAGRAQAQAKGL
                     FGEAQTITEFNEVTRLAEQAADLAMNAVAAAPSLLDRYITKAGDNLWNISKRPDVYGR
                     GAGWVKIWRANEKQIPDFDVLYF"
     gene            complement(557..1285)
                     /locus_tag="P5079_04985"
     CDS             complement(557..1285)
                     /locus_tag="P5079_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY29377.1"
                     /translation="MTAAGRPLAVVTGGSRGLGRAVCRLFASEGYAVAACARAAKDLR
                     ALKDEIQKSGGEIFVASADVGVEKDVRRFAKAVEGRFGRVDVLINNAGVLGPQRPLKD
                     CPSAAWGAAVRTNILGLFHVTKAFLPLFWRQGSGCIINVSSGAGKTGKARWGAYCVSK
                     FAMEGFTQVLAKETAERNIAVYSLNPGPTRTAMRAEAYPEEDPLTLPPPEKIAAAFLA
                     LARRNDVRRSGQYFEAREILAGDL"
     gene            complement(1282..2451)
                     /locus_tag="P5079_04990"
     CDS             complement(1282..2451)
                     /locus_tag="P5079_04990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY29378.1"
                     /translation="MKTVPPAKDTFDQIVRREIRRRGRVSFAEFMSWALYHPQHGYYM
                     AGPTRLGRAGDYFTSVQTGMMFGRLLCETFFEMWSLLGGNHFSLVELGAADGSLAEQI
                     LRTFQEKDRLKGLTYYLVEKSPAAREAARRRLSRFSRVKIFDDIASLEHISGMEGCVF
                     SNEFFDALPIHRVVREKAAFRELYVHEQNGVLSEEAGPPSTPLLERYLAEQRVTLEEN
                     QKAEICLALEDVVSEIERVLARGFVFSIDYGEPSGSLYRPERTDGTLQVTHRHALLSG
                     PFEKIGEADITAFVDFGRLAALGERGDLRALVFAPQGPYLVNSAQTLLKEIIETKIGR
                     ERDVSLAAQVQQLIHPQSLGGRFSVLIQGKNVGRPELSGGQVNRLRRLLPEKGPS"
     gene            complement(2448..3590)
                     /gene="queG"
                     /locus_tag="P5079_04995"
     CDS             complement(2448..3590)
                     /gene="queG"
                     /locus_tag="P5079_04995"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRY29379.1"
                     /translation="MVSESERVKELARELGFDFVGWTTAAPVASDGAFLDSWLRRGFA
                     ADMEWMRRRPEGRARPQSLLPNARTVIVLGVGYYQTEPSPRPAFPAGRVARYAWGRDY
                     HDVIGRRLKDFEMGLRKFFGSTVAFRSVVDAQPLLERAFARRAGLGFSGKNTNVLFPR
                     AGSWFFLSAVLLEAELPVPKTTPLTGCGACVRCLERCPTAALPEAYGLDARRCISYLT
                     IENRGAIAPEWRPLLGDRIFGCDTCQEVCPFNARPSETKWPGLASPGGVGAWLSLKEV
                     LSMRTEDLFRARFAGTALFRAKRAGLLRNACVAAGNVASEELAAELEECLRHDDSPLV
                     RGHAAWALSRAPKKKSRAALERALDAEPDAAVQEELRRALEPIPVP"
     gene            complement(3593..4354)
                     /locus_tag="P5079_05000"
     CDS             complement(3593..4354)
                     /locus_tag="P5079_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY29380.1"
                     /translation="MSKRIVGPWFVGHRGAMGHAPENTLVSFKTAWRMGADAVECDVH
                     MTKDRRLIVIHDGTLERTTNGAGRVFDHPWKQLRSLDAGKWFHPRFRGEKIRRLEELL
                     SWLRPKKSRAGRPMRIFIELKSKPVRYPGLAEAVARAVRRGGFLQRATVISFDHDLVR
                     RVKAASPRLQTGILFSEPLADPVSRARRVRADGLFPRYPLVNKALVDSAHRRGLLVGA
                     WTVNEKADMLRMARLGVDAVTSNFPDRLIRVLRRK"
     gene            complement(4360..5379)
                     /locus_tag="P5079_05005"
     CDS             complement(4360..5379)
                     /locus_tag="P5079_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY29381.1"
                     /translation="MLREALEFLVSPCCRRELSLAAGPGKSSDIDVDEGTLTCPCGRI
                     YPIVHGIPVLIREETLSDPVFENNRKTWNALWKKTRDMASTFFTSGEMFRYDFQCDNS
                     IATGAVVFEAGVGQGKYVSYLRDASPAWLIGVDAAEYLHEIWDNLKSGPRFSCKICLV
                     RADLNDLPIRSGKFDYVIASRVLHHLPRMDKSLSDLLSLLKPKGKMLAVVYAAGGSFR
                     HVIDAVERIKKILNVLCSLDQMYVISFFVSAVIYAVILLFYVPLGRFLSVRRLLPLGD
                     VMLFWKKIGFRGFWKYIVFDILVAPETRYTVEEDLKKILSNLPVSRRSYRRQHEAMWS
                     IELER"
     gene            complement(5404..7311)
                     /locus_tag="P5079_05010"
     CDS             complement(5404..7311)
                     /locus_tag="P5079_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29382.1"
                     /translation="MEWVRAACRFDDRSLQEQLLVRSVETIPAWEVSLWTLLETRGPK
                     AEEIVLSDLSLPGSAGPVPAPSEIVVWDHPSETRSDFSESSGRYPIAATLKRCDCPAC
                     GRSGSISCGVCSGYGKTACPNCRGAGKIACEYCKGLGKTNCLKCDGKGVNKGAGVVVH
                     AANACDACRGAGKVACTRCVNGEVPCSQCAGAGHQPCSKCRANGKSPCPTCQGKGEIL
                     KGYGFQADFRLRKMTTVFSALPAPKSLLDAVFSQKRNDPPEQKDAVPLLQAEHFAALP
                     FPAEARQNLERYSQNLPSTLDANTRMVRQRAAWHSADLCRTTGTFCGQEFVYWVDISS
                     GKVVAEKDPVKDLSAASEQEAEKAWERKEWAAALSLARQTLLFEPDNPQAKRIVDQCR
                     RRMRGESMMLAAGAATAVSLLAAGGIFILEKGLNRSGPVLRVAVFGLIAGTVLGWIWD
                     RLAAEKFPVRKRRLASFFGTGGLLICGFLILRWGAGWNPVRSADEAAFQKEWTTRFPS
                     GGPGEVYWEPDLRFLEELAEKYKGTQVDLGRVKSGIEKQRRLKEQRKEQEEELSSRLS
                     ALMADAGLNFQDKLRRLTELRDLYQLQSVNVDSVAEAIKKVEEEKKLKEAERPRGRIS
                     IVPAPYKRNRR"
     gene            complement(7418..7789)
                     /locus_tag="P5079_05015"
     CDS             complement(7418..7789)
                     /locus_tag="P5079_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29383.1"
                     /translation="MDKKRILLVEDDEDVSDFLKPFLADNDYEMDVAIDGRSGLKKLG
                     EFQPDLVILDLMMPGVDGFEFLKSLQAKPELKTAKILVVTAVQKVGGAEEALRLGADG
                     YLMKPVDNDLLLEKIKDLLSS"
     gene            complement(7889..8977)
                     /locus_tag="P5079_05020"
     CDS             complement(7889..8977)
                     /locus_tag="P5079_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY29384.1"
                     /translation="MSNPKHRPPPSRWPFRKRFVYSGVGVLGGLLLLELLLRIFQFRY
                     STLPMSMEYVQWISALGHSQEMPRGTFRINYRADPEYLWYPEPTAGFTNSLGFRGPEW
                     AREKPAGVSRVVCLGDSCTATGEIPYAERLQSCLNAAGQRFDVMNAGVGSWSSYQGRL
                     LLGKKILDFKPDVVTIYFGWNDHWLAWSHSDRDLGKYLSGRARWINLVRKSRLAQALT
                     WAANRLLPRPDPRLRQPPLFRVSEEDYRKNLEAMVSSARTAGALPILLTAPTTLHAEH
                     GVTRYLVTQSRLFHNTQQINQVHDAYNNVVRNVAAATETPLIDLAQEFIYIEDKNDYI
                     SDGIHLTPRGAEWVAQQICLAIRDRSRD"
     gene            complement(9009..10301)
                     /gene="purB"
                     /locus_tag="P5079_05025"
     CDS             complement(9009..10301)
                     /gene="purB"
                     /locus_tag="P5079_05025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRY29385.1"
                     /translation="MIARYTRPEMAQIWSEQFRWETILEVELLAAEAMARRGRLDWKS
                     VRTLRKKSRVRVARIREIEKTVKHDVVAFLTHIEEQAGPAAKALHAGLTSSDVLDTAL
                     AVQLKKSTELLTAGLKELQSAARRLALRHRNTVMMGRTHGVHAEPITFGLKAAGWYAE
                     LKRDMARLRSAGEEVAVGKISGAVGTFAHVDMAVESEVCRRLGLRAEPVSTQIVPRDR
                     HAFYLQTLAVVGAGLERIATEIRHLQRTEALEVEEPFSAGQKGSSAMPHKRNPVASEN
                     ICGLARLLRAYAGAALENVALWHERDISHSSVERVILPDAAAALDFAVHRLADVLNGL
                     QVYPDRMRTNMAKTAAVICSQRLVVSLAQKGLPKQKAYDLVQRHAQRAWKSGEDFWAL
                     VGSDPRIVRRLSKAELRRCSDLSFFLKSVGRIIHRALF"
     gene            complement(10298..10951)
                     /gene="purN"
                     /locus_tag="P5079_05030"
     CDS             complement(10298..10951)
                     /gene="purN"
                     /locus_tag="P5079_05030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRY29386.1"
                     /translation="MSVSLDRAVTVVPKHRIGVLASGRGSNFESLVTATRNGFIPDAE
                     VGLLVCNRPAAGAVQRAERLGVECLVMEPLPFADEAVYFERIADEFQRRGVTLVCLAG
                     FLLKVHAAMLRRFPGRILNIHPALLPKYGGRGMYGRRVHEAVLRAEEKETGCTVHLID
                     DEYDHGPTVLQAVVPVRPGDTPETLAARVSEKEHVLYPEAVKRVLLGLSGKSEGAGK"
     gene            complement(10935..11909)
                     /gene="purM"
                     /locus_tag="P5079_05035"
     CDS             complement(10935..11909)
                     /gene="purM"
                     /locus_tag="P5079_05035"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRY29387.1"
                     /translation="MKRGRRWTYRRAGVDIAAGDELVERLRKKFPHIGDFAGLFPLKT
                     RLGRNPLLVGCADGVGTKLNIAQALKVHDTVGIDLVAMNVNDLICSGARPLFFLDYFA
                     AGRLDVDVADRVLGGIAVGCRQAGCVLLGGETAEMPGMYRPGEYDLAGFAVGILDASE
                     RVRNDRIRAGDVLLGLPSSGVHSNGFSLVRRLFRGADLKKWGKTLLTPTRIYVDDIGR
                     VLKKYPGQINGIAHITGGGLPENVPRFLPKKCDAVLFRASWPLPEVFSEIQRRGRVED
                     PDMFRTFNMGLGMVLAVSPAAAVRVRKDLAPCFEVGRVSPGTGLVRFA"
     gene            complement(11911..>12418)
                     /gene="pyrE"
                     /locus_tag="P5079_05040"
     CDS             complement(11911..>12418)
                     /gene="pyrE"
                     /locus_tag="P5079_05040"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRY29388.1"
                     /translation="SLFYLQCAKVLQYPDLAARLGEELAKKCHGLGKKISVVLSPAVG
                     GLILGHEVARPLSARCLFAEREKSSGLFSLRRGFQLKRGDEVLLVDDVLTTGGSLREL
                     AQLAAAAGAKVAGIASLAERGSHVVDFAVPRRTLIKLDFEDYAPADCPACRNRLPLEK
                     PGSKPEAS"
BASE COUNT         2532 a         4006 c         3641 g         2239 t
ORIGIN      
        1 cgaaataaag cacatcgaaa tcggggatct gtttctcgtt ggcccgccat atcttcaccc
       61 agcccgcccc gcggccgtag acgtccgggc gtttggaaat gttccacagg ttgtcccccg
      121 ccttggtgat atagcggtcg agcaaggaag gcgccgcggc gaccgcgttc atggccaggt
      181 cggccgcctg ttccgccaaa cgagtcacct cattgaattc cgtgatggtc tgggcttccc
      241 cgaacaagcc cttggcctgc gcctgggcgc gaccggcggc ggtggcgtcg cgctcttcct
      301 tttgggcagt ttcaatggcc aaatcggcgt agcggaggga ttgttcggcg cgttcccgcg
      361 ccaccgccag ttgtttccgt tcctccgccg aacggcgttc ctgctcgagc atgtccgcgg
      421 tgagcgccgg aaccgccggc gccgctttcg gcagagacgt ccatgagggc agaaagtgcc
      481 gataggcgaa aaacccggcc gccgagagga aaaagaaact aaagatgaga tggaggttgg
      541 ttatccgcat cgtcctttaa aggtcccctg ccagaatttc ccgtgcttca aaatactgtc
      601 ccgaccgacg cacgtcgttc cggcgcgcca gcgcgaggaa agcggcggcg atcttctccg
      661 gcggcggcag cgtgagcggg tcttcctcgg gataggcctc cgcccgcatg gcggtccgcg
      721 tgggtccggg gttgagggaa taaaccgcga tgttcctctc agcggtttcc ttcgccaaca
      781 cctgggtaaa gccttccatg gcgaatttgg acacgcagta ggccccccag cgcgctttgc
      841 cggttttccc ggcgccggaa ctcacgttta ttatacaacc tgaaccctgc cgccagaaga
      901 gaggaagaaa agcttttgtg acatggaaga ggccgagtat gttggtgcgg acggcggccc
      961 cccacgcggc ggacgggcaa tctttcagcg gccgttgcgg tcccaaaact ccggcgttgt
     1021 tgatcaacac atccacccgg ccgaaacggc cctccaccgc cttcgcaaac cgccggacat
     1081 ccttctccac gcccacgtcg gccgaggcga caaagatctc tccgccgctc ttctggattt
     1141 cgtctttcaa agcccgcaag tcttttgcgg cgcgggcaca ggcggccacc gcataccctt
     1201 cggatgcgaa cagccgacag accgcccttc ccagcccacg ggaaccgccc gtaacaacgg
     1261 ccagcgggcg gccggcggcg gtcacgacgg tcccttttcc ggcaagagcc ggcgaagccg
     1321 gttcacttgc ccaccggaaa gttcgggcct tccgacgttt ttcccctgga tcaaaacgga
     1381 aaaacggccg cccaggcttt gcggatggat gagctgctgc acctgggccg ccagggagac
     1441 gtcccgttca cggccgattt tggtttcgat gatctctttc aacagcgtct gggcggaatt
     1501 caccaggtag ggcccctgcg gcgcgaagac gagggcgcgc aggtccccgc gttcgcccag
     1561 ggcggccaac cggccgaaat ccacgaacgc ggtgatgtct gcctcgccga tcttttcgaa
     1621 cggcccggag agcaaggcgt gccgatgcgt cacctgcagg gtcccgtccg tcctctccgg
     1681 ccgatagagg gaaccggacg gctcgccgta gtcgatgctg aagacgaacc cccgggccaa
     1741 aacccgttcg atctccgaaa caacgtcttc cagggccagg caaatctccg ctttctggtt
     1801 ttcctccaga gtcacgcgct gttcggcgag ataacgctcc aacagcggcg ttgaaggggg
     1861 gccggcctct tccgacaaaa caccgttctg ttcatggacg taaagttcac gaaacgccgc
     1921 cttttcccgc accacgcgat ggatcggcaa agcatcgaaa aattcgttgg aaaaaacgca
     1981 tccctccatg ccggagatat gttccaagga agcgatgtcg tcaaaaatct tgacccggga
     2041 aaaacgggag agccgccgcc gggcggcctc ccgggcggca ggactctttt caacgaggta
     2101 gtaggtcaat cccttgaggc ggtccttctc ctggaacgtg cgcaaaatct gttcggccaa
     2161 ggagccgtcg gccgcgccca actcgacaag gctgaaatga ttcccgccga gaaggctcca
     2221 catctcgaaa aaagtctcgc acaacagacg tccgaacatc attccggtct gcacgctggt
     2281 gaagtagtcc ccggcgcggc cgagtctggt tgggccggcc atgtaatagc cgtgttgcgg
     2341 gtgatagagg gcccaggaca tgaactccgc gaacgagacc cggccgcggc ggcggatctc
     2401 ccggcggacg atctggtcga aggtgtcttt tgccggcgga acggttttca tggcacggga
     2461 atcggttcca aggcgcggcg caattcctcc tgaacggccg cgtcgggctc cgcgtccaaa
     2521 gcacgttcca acgcggcccg gctttttttc ttcggggccc gcgacagggc ccaggccgcg
     2581 tggccacgga caagcgggct gtcgtcgtgc cggagacatt cctccagttc cgcagcgagt
     2641 tcctccgacg ccacgttgcc cgccgccacg caggcgttgc gcagcaaccc ggcgcgcttg
     2701 gcacgaaaca gcgcggtccc ggcgaaccgc gcccggaaga ggtcttctgt cctcatggac
     2761 aacacttcct ttaaagacag ccacgcgccg actcccccgg ggctggcgag tcccggccat
     2821 ttcgtctccg aaggtctcgc gttgaacggg cagacttcct ggcacgtatc gcaaccgaaa
     2881 atccggtccc ccaggagggg gcgccattcg ggagcgatcg ccccccggtt ttcgatggtg
     2941 agataggaaa tacagcggcg ggcatcgagg ccataggctt cgggcagggc ggcggtgggg
     3001 catctctcca aacatcggac acaggcgccg caccccgtca aaggggtggt tttaggaacg
     3061 ggcaattccg cctccaggag caccgccgac aaaaaaaacc acgaacccgc gcgggggaag
     3121 agcacattgg tgttttttcc cgaaaaaccc aaacccgccc gacgggcaaa agcccgttct
     3181 aaaagaggct gagcgtccac cacagaccgg aaagcgaccg tcgatccgaa aaatttccgc
     3241 aggcccattt caaaatcctt cagccgacga ccgatgacgt cgtgataatc gcgtccccaa
     3301 gcatagcgag cgacgcggcc ggcgggaaat gcgggacggg gagagggttc cgtttgataa
     3361 tagccgacgc ccagaacgat cacggtgcgg gcgttgggca aaagggactg cggccgggcg
     3421 cgcccctcag gccggcgacg catccattcc atgtccgccg caaagcctcg ccgaagccaa
     3481 gaatcgagga aggctccgtc cgacgcgaca ggagccgcgg tggtccaccc gacgaaatcg
     3541 aaacccaatt cccgggccag ctccttgact ctctcggatt cggaaaccat ttttatttac
     3601 ggcgaaggac gcggatcaga cgatcgggga aattggaggt gaccgcgtcc acgcccagcc
     3661 gggccatccg gagcatgtcg gctttctcat tgacggtcca cgcgccgacg agcaggccgc
     3721 ggcggtgggc cgaatccacc agggccttgt tcaccaaagg ataccgtgga aaaagtccgt
     3781 ccgcccggac ccggcgtgcg cgcgacaccg gatcggccag cggctcggaa aagaggatgc
     3841 cggtctgcag gcggggagac gccgctttga cccggcggac cagatcgtgg tcgaaggata
     3901 tcaccgtggc ccgctgaagg aatccgccgc gccgcacagc ccgggccacc gcctcggcca
     3961 gaccggggta tcggaccggc ttgcttttaa gttcgatgaa aatgcgcatc ggccgccccg
     4021 cgcgggattt cttcggccgc aaccaggaga ggagctcctc caaccgccgg attttctctc
     4081 ctcggaacct cgggtggaac cacttccccg catccaacga ccgcaattgt ttccagggat
     4141 ggtcgaacac gcggccggcg ccgttggtgg tgcgttccag ggtgccgtcg tggatgacga
     4201 tcaaacgacg gtctttggtc atatgcacgt cgcactcgac cgcatccgcg cccatccgcc
     4261 aggcggtctt gaacgaaacg agcgtatttt cgggagcatg ccccatggcc ccgcgatggc
     4321 cgacgaacca agggccgacg atgcgtttcg acatgggcgt caacgctcca actcgatgga
     4381 ccacatggct tcgtgctgcc gccggtagga ccggcgggaa accggcaggt tcgacaatat
     4441 ttttttaaga tcctcctcga ccgtataccg ggtttccggc gccaccagga tatcgaacac
     4501 gatgtacttc caaaagccgc ggaaacctat ctttttccag aaaagcatga cgtcccccaa
     4561 cgggaggagg cgccgcaccg atagaaaacg gcccagcggg acatagaaca aaaggatgac
     4621 ggcatagatc acggccgaga cgaagaatga aataacatac atttgatcca gggagcacaa
     4681 gacgttgagg atttttttta ttctctccac cgcatcgata acgtggcgga acgatccgcc
     4741 ggcggcgtat acaacggcca gcatcttccc cttaggcttg agaagagaaa gaaggtcgga
     4801 aaggcttttg tccatccggg ggagatggtg caaaacccgc gaggcgatga cgtaatcaaa
     4861 tttccccgat cggatcggca aatcgttcag gtccgcccgg accaggcata ttttgcagga
     4921 aaaccggggg cctgatttca gattgtccca tatctcatgc aaatattcgg cggcatccac
     4981 gccgatcagc caagccgggg aggcgtctcg caggtaggag acatacttcc cctgccccac
     5041 ccccgcctca aaaacaacgg cgccggtcgc gatggaattg tcgcactgga aatcataacg
     5101 gaacatctcc ccggacgtaa aaaaagtgga agccatgtcc cttgttttct tccaaagggc
     5161 gttccaggtt ttccgattgt tttcaaagac cgggtccgac aacgtctctt cccggatcaa
     5221 aaccgggatc ccgtggacga tgggataaat ccgtccgcaa gggcacgtca aagtcccctc
     5281 gtcgacatcg atatccgaag atttccccgg cccggccgcc aaagacaact ccctccggca
     5341 acagggactg accagaaatt ccagggcctc tctcagcatg ttctttcggc gggtcggaga
     5401 cggtcagcgg cgatttcttt tatagggggc cggaacgatg gagatgcggc cgcggggccg
     5461 ctcggcttcc ttcaacttct tctcttcctc cacctttttg atggcctccg cgacggaatc
     5521 cacgttgacc gattggagct gatagaggtc tctcagttcg gtgaggcggc ggagcttgtc
     5581 ctgaaaattc aaaccggcgt cggccatcag cgccgacaat ctcgaggaaa gctcttcctc
     5641 ctgttccttc ctctgctctt tcaagcgccg ttgtttttcg atacccgact taacccgtcc
     5701 cagatccacc tgcgttccct tgtatttttc cgccagttcc tccaaaaacc ggagatccgg
     5761 ctcccaatag acttcgccgg ggccgccgga aggaaaccgt gtcgtccatt ccttttgaaa
     5821 cgccgcctcg tcggcgctgc gcaccggatt ccatcccgct ccccaacgca aaatcaggaa
     5881 cccgcaaatc agcaggcccc cggtgccgaa gaaagacgcc aagcggcgtt ttctgacggg
     5941 gaatttctcc gcggcgagcc ggtcccaaat ccatcccaaa accgtcccgg cgatcaagcc
     6001 gaaaacagcg acccggagga ccgggccgga acggttcaac cctttttcca atataaaaat
     6061 accgccggcc gccaggaggg acaccgcggt cgccgcgccg gcggccaaca tcatcgattc
     6121 ccccctcatt cgacggcgac attggtcgac gatccgcttg gcctggggat tgtccggttc
     6181 aaacaagagc gtctgccggg cgagcgaaag cgccgccgcc cattccttcc tttcccaagc
     6241 cttctccgcc tcctgttcgc tggcggccga gagatctttt acgggatctt tttcagcgac
     6301 gaccttgccg ctggagatat ccacccaata aacgaactcc tggccgcaga aggtccccgt
     6361 cgtccgacag agatcggccg aatgccaggc cgcccgctga cgcaccatgc gggtgttcgc
     6421 gtccagcgtc gacggcaggt tctgcgaata acgttccaga ttttgccggg cctccgccgg
     6481 gaagggaagg gcggcgaaat gttccgcctg caggagagga acggcgtcct tctgttcggg
     6541 gggatcgttc cttttttgac taaaaacggc gtccaacaag gacttggggg cggggagcgc
     6601 ggaaaacacc gtcgtcattt ttcgcagacg gaaatccgcc tgaaatccat atcccttcag
     6661 aatctcgcct ttcccctgac atgtggggca aggcgacttg ccgttcgccc ggcacttgga
     6721 acacggctga tggcccgccc cggcgcattg ggaacagggg acctcgccgt tcacgcagcg
     6781 ggtgcacgcc acttttcccg cgccccgaca ggcgtcgcag gcgttggcgg cgtgcaccac
     6841 gacgccggcg cctttgttga cgccctttcc gtcgcatttc aaacaattcg tcttccccaa
     6901 ccccttgcaa tattcgcaag cgattttccc cgcgccccga cagttgggac acgccgtctt
     6961 gccgtatccg gagcaaacgc cgcaggaaat ggagccgctc ctgccgcagg cgggacagtc
     7021 gcatcgcttg agggtggcgg cgatcggata acggccgctg gattcggaaa aatcggaccg
     7081 ggtttcggag ggatggtccc acacgacgat ctccgacggg gccgggacgg ggccggcgct
     7141 tccgggcagg gacaaatcgc tcaaaacgat ctcctcggcc ttcggaccgc gcgtctccaa
     7201 caaggtccaa agcgaaacct cccaggccgg gatcgtttcg acggaacgaa cgagcaattg
     7261 ttcctgcaga ctccggtcgt cgaaccggca ggcggcgcgg acccattcca tgaggatttt
     7321 ccgcaacgcg tccggaacgg cagaagaggg aaccgttgga ttgtttgggg gggcactcat
     7381 attttcaagg gaggcgctgg tcgttcgggc ggaggattca gctcgagagg aggtccttta
     7441 ttttttcgag caaaaggtcg ttgtccacgg gcttcatcag atagccgtcg gcccccagcc
     7501 ggagcgcttc ttcggcgccg cccacttttt gcacggccgt gaccaccagg atcttcgccg
     7561 tcttcagttc cggcttcgcc tggagggact tcaaaaactc aaacccatcc acccccggca
     7621 tcatcaggtc gaggatcacc aaatcgggct gaaattctcc caactttttg agaccggaac
     7681 gaccgtcgat ggccacatcc atctcgtagt cgttgtcggc caggaacggt ttcaggaaat
     7741 ccgaaacgtc ctcgtcgtct tcaacaagca atatcctttt tttatccatg ccgccccctt
     7801 ggccgatata tcaagggaca ttattactat ttttgtggaa ttagtcgaac gtgacccgtt
     7861 taaaaaaacc gccggatatc ccgctctttc agtccctcga acggtcgcgg atggcgagac
     7921 agatctgttg cgccacccat tcggccccgc gaggagtcaa atggatgccg tcgctgatat
     7981 aatcgttttt atcctcgata tagatgaatt cctgcgcaag gtctatcagc ggcgtctccg
     8041 tggcggccgc gacgttccgg acgacgttgt tgtaggcatc gtgcacctgg ttgatctgct
     8101 gggtgttgtg gaagagccgg gactgggtca ccaggtagcg tgtcacccca tgttccgcgt
     8161 gaagggtggt gggagcggtc aacaaaatcg gcagggcccc ggcggtccgg gcggacgaca
     8221 ccatggcttc cagattttta cggtagtcct cctcgctgac gcggaaaaga ggcggctgac
     8281 ggagccgtgg gtcgggccgg ggcagaagcc ggttcgccgc ccacgtcaac gcctgcgcca
     8341 aacggctttt cctcaccaaa ttgatccacc gcgcccgacc ggaaagatat tttcccaaat
     8401 ccctgtccga atgactccac gccagccagt ggtcgttcca tccgaaataa atggtaacca
     8461 cgtccggttt gaaatccaga atctttttcc ccagcaaaag ccggccctga taggaactcc
     8521 aggaccccac gccggcgttc atcacatcaa aacgctggcc ggcggcgtta aggcaggact
     8581 ggagccgttc ggcgtaaggg atctcccccg tggccgtgca ggaatccccc agacagacca
     8641 cacgggagac tcccgccggt ttttcgcgag cccattccgg tccgcggaac ccgagcgaat
     8701 tggtgaaacc cgccgtgggt tccggatacc acagatattc ggggtccgcg cggtaattga
     8761 tacggaaagt gccgcgaggc atctcctggc tgtgtcccaa ggccgaaatc cactgtacgt
     8821 attccatgga catgggcaag gtggagtaac ggaactggaa aatcctcaag agcagttcca
     8881 ggaggaggag cccgcccagg acgccgacgc cggaatagac aaaccttttc cggaacggcc
     8941 aacgggacgg cggaggacgg tgttttgggt tggacacgcg gagaacgaaa aaagggacgg
     9001 ggattccctt aaaaaagcgc ccggtggatg atccgtccga cgcttttaag gaaaaacgac
     9061 aggtccgagc aacggcgcaa ttcggccttg gaaagacgcc gaacgatacg cggatccgac
     9121 cctacaaggg cccagaaatc ctccccgctt ttccatgccc gctgcgcatg ccgttgcacc
     9181 aggtcatagg ctttctgttt gggcagaccc ttttgggcca gagacaccac cagacgttgg
     9241 gagcatatca cggcggcggt cttggccatg ttggttcgca tgcggtccgg atagacctgc
     9301 agaccgttca agacatcggc gagacggtgg acggcgaaat ccagggccgc ggcggcgtcc
     9361 ggcaggatga cacgctcgac ggaagagtgc gagatgtcgc gctcatgcca caacgccacg
     9421 ttctccagcg ccgcgccggc gtatgcgcgc aacaaccgcg ccaaaccgca gatgttctcg
     9481 ctggccacgg gattgcgctt gtgcggcatg gcgctcgatc ccttttggcc ggcggaaaaa
     9541 ggctcctcca cctccagcgc ctcggtacgc tgcagatggc ggatctcggt ggcgatgcgt
     9601 tccaggccgg cgccgaccac cgccagggtt tgaaggtaga acgcgtgccg gtcgcgcggc
     9661 acgatctggg tggacaccgg ctccgcccgc aaccccagcc gacggcacac ctccgactcc
     9721 accgccatgt ccacgtgggc gaaggtcccc acggcgccgg agattttccc gacggccacc
     9781 tcttcccccg ccgaccgcag gcgggccatg tcgcgtttca attccgcgta ccaccccgcg
     9841 gccttcaatc cgaacgtgat cggttcggcg tgcaccccgt gcgtgcggcc catcatgacc
     9901 gtgttcctgt ggcgcagggc cagccgccgg gcggcggact gcaattcctt caaccccgcc
     9961 gtcaaaagtt cggtggactt cttcagctga acggccaggg cggtgtccaa aacgtcggaa
    10021 gaggtcaagc cggcgtggag cgccttggcg gccggtcccg cctgttcctc gatgtgcgtg
    10081 aggaaggcga cgacgtcgtg cttgacggtc ttctctattt cccggatccg cgcgacgcgg
    10141 acccggctct ttttccgaag cgtccgcacg gacttccaat ccaagcgacc gcggcgggcc
    10201 atggcctccg ccgccagaag ttccacctcc aggatcgtct cccagcggaa ctgttccgac
    10261 catatctgcg ccatctccgg acgcgtgtat cgtgcgatca ttttcccgct ccttccgatt
    10321 ttccggataa acccagcaat acccgtttca ccgcctcagg atacaaaaca tgttcctttt
    10381 ccgaaaccct cgccgccaaa gtttccggtg tatcgcccgg ccgcacgggc accaccgcct
    10441 gcagcaccgt cgggccgtgg tcgtattcgt cgtcgataag atggacggtg cagccggtct
    10501 ccttctcctc cgcccgcagg acggcctcat ggacgcgccg gccgtacatg ccccgcccgc
    10561 cgtatttggg aaggagggcg ggatggatat tgagaatgcg gccgggaaac cgccgcagca
    10621 tcgccgcgtg cactttcaaa aggaaccccg ccaaacacac cagggtcacg ccgcggcgct
    10681 gaaattcgtc ggcgatccgc tcgaaataga ccgcctcgtc cgcgaaaggg agcggttcca
    10741 tgaccaggca ttccacgccc aggcgctccg cccgctggac agccccggcc gcgggacgat
    10801 tgcaaacaag caggccgact tccgcgtcgg ggataaatcc gttgcgagtc gcggtcacca
    10861 gactttcgaa gttcgagccc cgcccggacg ccagaacgcc gatacggtgc ttggggacga
    10921 ccgtcacggc ccggtcaagc gaaacggaca agtcccgtgc ccggcgagac gcggcccacc
    10981 tcgaaacacg gggccaggtc ttttcggacg cggacggccg cggcgggaga aaccgccaac
    11041 accatgccca ggcccatatt gaacgtgcgg aacatgtctg ggtcttccac ccgaccgcgc
    11101 cgctggatct ccgaaaaaac ctccggcaaa ggccaggaag cccggaagag gacggcgtcg
    11161 cactttttcg gaaggaacct cggcacgttc tccggcaacc cgccaccggt gatatgggcg
    11221 atgccgttga tctgtccggg atattttttc aggacgcggc cgatatcgtc cacataaatg
    11281 cgcgtcggcg tcaaaagggt cttcccccat tttttcagat ccgccccgcg aaaaagtctc
    11341 cgcaccaggg aaaaaccgtt ggaatggacc cccgacgaag gcaaacccag cagcacatcg
    11401 ccggcccgga tacggtcgtt ccgaacgcgt tccgaggcgt ccaggattcc caccgcaaaa
    11461 ccggccagat cgtattcccc cggccggtac atccccggca tctcggcggt ctcaccgccc
    11521 agcagcacgc accccgcctg acggcacccg accgcgatgc cgccgagaac gcggtcggcc
    11581 acgtccacat ccagccggcc ggcggcgaaa taatccagga agaacaacgg gcgcgcaccg
    11641 gaacagatca gatcgttcac gttcatggcg acgagatcga tgcccacggt gtcgtgcacc
    11701 ttcagggcct gggcgatatt caacttcgtc cccacgccgt cggcgcagcc gaccaacagg
    11761 ggattccgcc ccaatcgggt tttgagcgga aaaaggccgg cgaagtcgcc gatgtgaggg
    11821 aactttttcc gaagccgctc gacgagttcg tcgcccgccg cgatatccac ccctgcccgt
    11881 cggtatgtcc agcgccggcc gcgtttcata tcaggaggcc tccggtttgg agccgggctt
    11941 ctccagggga agccggttgc ggcaggccgg acaatcggcc ggggcgtaat cctcaaaatc
    12001 caattttatc agcgtccggc gcggcacggc gaaatcgacg acatgactcc cccgttcggc
    12061 caaagaagcg atccccgcga ccttcgcgcc ggcggccgcc gccagctgcg ccagctcgcg
    12121 cagggagcct cccgtcgtca acacgtcgtc caccaagagg acttcgtccc cccttttcaa
    12181 ctgaaaaccg cggcgcaacg aaaaaagacc ggaagacttt tcccgttccg cgaacagaca
    12241 gcgggccgac aagggccggg ccacctcatg tcccagaatg aggccgccga ccgccggaga
    12301 cagcacgacg gagatttttt ttcccaaacc gtggcatttt tttgcgagtt cctcccccaa
    12361 acgggccgcg aggtcggggt actgaagcac cttggcgcat tgcagataaa aaagactg
//
LOCUS       DAOTFD010000057        12404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3578_length_12404_cov_4.078954, whole genome shotgun sequence.
ACCESSION   DAOTFD010000057 DAOTFD010000000
VERSION     DAOTFD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3578_length_12404_cov_4.078954"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="P5079_05045"
     CDS             <1..546
                     /locus_tag="P5079_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29389.1"
                     /translation="TGLLVVPLLFAGWSFKIFRADQCLAQAKTAMETNDLDRASSRIE
                     AGLFWNPQEAELYSLRSALKAFAGDWAAADDNVRKSIRLSPFVSRFHREAGEIALRRG
                     RREEALLYFETASRLLPRDIAPWLRIAQLQEDAGRLPAAVEAYERVFRFMEEKPARDA
                     GWETELSVKLSNLKEKLTHAP"
     gene            536..1681
                     /locus_tag="P5079_05050"
     CDS             536..1681
                     /locus_tag="P5079_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29390.1"
                     /translation="MPRKIKVAHIVTLLEFGGAQQNTLYTVEHLDRNRFEPLLLCGRG
                     AYLDGDAEKSGYPTIFINSLDRPLRPLRDMLAFLEIFFVLKREKPDIVHTHSSKAGVL
                     GRWAAKCAGVPFIVHTFHGFGFTSRQNIWLRWTFILIEKISAYAAHVLLAVSQANREE
                     ALRHGIGRPDKYRLLRSGIARPLFQSLSRRPNAPEGLPLTPDLRLVTTIGPFKPQKNL
                     SDFVAMAGRVHAKHPEARFLIVGDGELRPLLEQEIERHGLTAVFYLPGWRRDIPHIMM
                     RSNIFVLTSLWEGLPRALVEAMCAALPCVANAVDGVNDIVNDGANGFLVPPRRPDLTA
                     ERVNHLLENPNFASEMGHRARQSIGDEFDIDEMVRRQEELYSAAVHP"
     gene            complement(1800..2678)
                     /locus_tag="P5079_05055"
     CDS             complement(1800..2678)
                     /locus_tag="P5079_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29391.1"
                     /translation="MMGAGLFLAGLPPVVRAENQQFQVVPPPLPMMRLLTDWAELSND
                     LIYLKAKAGGITLKGGGYTGVFNAITSTHTGVAVNFAFIGMGGDLPSNYSSMAGFQGD
                     MGFSVLSEGVNFVVDPLSTPARNQKGWALPISVGPTLTMMNSSIVTSYLRTNTSGTIV
                     SEDDTVDIFGVLYGWQAGIGVGIPLDRNNYLRLFPFATLNQNLGGSFTVTTDLTSTSA
                     DAEKSPPSTSYGMDIQIPRWNLSVGSIIQQMKKQKEGDDDVKTTMYRLSWTLRFSAAN
                     RQARLEKIKAGRSQSK"
     gene            complement(2695..3441)
                     /locus_tag="P5079_05060"
     CDS             complement(2695..3441)
                     /locus_tag="P5079_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29392.1"
                     /translation="MRRFILPLGFLLVGVCGCSTGRHKTEVQAKAAHLEVQRSVANAA
                     VSAAASANSELAWATYYVDIASAAAFTAYDASSGYYTVKVYSNATEQIRYEDSAGDAL
                     AVMGTASPFSKNAGLAATWKTTRVRDTSNGKNVTRDITAIKMNGTVQTGVTLANGTVN
                     TITNIATPLMTWLPYTGTLTGGNFSIVFSTGVGGGTITDSGVRMNVLVTPTGTGSSST
                     RFRYTFSVTTMEAAAPTAFNPVSRLGYGNE"
     gene            3597..4058
                     /locus_tag="P5079_05065"
     CDS             3597..4058
                     /locus_tag="P5079_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRY29393.1"
                     /translation="MKLRVHEILDAGVIDRTDTLPAADIPLDLPDNPVLLDPVETKIH
                     AEKLEEEVLVWGEVSAKVRLTCSRCLEPYEEEPLGGRFEVRAPARQNFVDVGEEIRQA
                     LLLAIPPKPLCRANCKGLCPKCGKNLNQGVCGCPSRPGESPFAKLRDLKIQ"
     gene            4074..4280
                     /gene="rpmF"
                     /locus_tag="P5079_05070"
     CDS             4074..4280
                     /gene="rpmF"
                     /locus_tag="P5079_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRY29394.1"
                     /translation="MANPKKKHTPMRRDMRRAQNWRLELGSLSRCPHCGGMRPPHRLC
                     PACGYYGAELILPPKVKKDKKEKS"
     gene            4360..5391
                     /gene="plsX"
                     /locus_tag="P5079_05075"
     CDS             4360..5391
                     /gene="plsX"
                     /locus_tag="P5079_05075"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRY29395.1"
                     /translation="MSVRIALDAMGGDLGLAVNVEGALQAKRELGVEVTLVGDQSLLE
                     EELDRHNARGELAIVHAPTVVGMHEKPAEACRAKKDSTIMLCAQMVAEGRADAYVSAG
                     NSGAAMTAALWHLRRIPGVSRPAIATLFPTLTGSTVILDVGANVDCKPKHLAQFALMG
                     SIFARSAFNVDQPRVGLLTIGEEEGKGNLLTQETHEMLKKNPLINYIGHVEGRDIPAG
                     EVHVFVCDGFVGNVVLKFGEGLAAAVIHLIKTEIKKHPLAIFGRYLLKGAFREMRKRT
                     DPSEHGGAPLLGVNGVAIIAHGGSNAKAIKNALRAAAFFHEKGVNRQIHDQIHQLDTT
                     QLVPQKATA"
     gene            5396..6403
                     /locus_tag="P5079_05080"
     CDS             5396..6403
                     /locus_tag="P5079_05080"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRY29396.1"
                     /translation="MTGIKFLSTGVGLPENVLTNADLEKMVETSDQWITERTGIKTRR
                     IASDKQATSDFSIAAARQALSRAGIAPETVDAVVVATCTPDHLMPSVACLVQRALGLK
                     NAFAFDLEAACSGFIFGLACVNGLMRSGAAKTVLLIGADTLSKFTDWTDRGTCVLFGD
                     GAGAALLQASDRDDNLLAVHLGSDGSFTDILNIPGGGSRHPLQRSKNAPFTTGEVAGN
                     FPPYIHMEGKEIFKNAVLRMAEAADSALKKCGKTSDDLRLLIPHQANLRIIDAVAKRM
                     KLGDDKVFRNVQKYGNMSAATTVIALDEAVQGGVVKRGDLVELIAFGGGLTWGAAVLR
                     W"
     gene            6466..7227
                     /locus_tag="P5079_05085"
     CDS             6466..7227
                     /locus_tag="P5079_05085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRY29397.1"
                     /translation="MAQRLKDGEIDVVVTSPPYNLGIRYTRYRDNRPYHDYLSWLECV
                     ARGIKRVMRRDGSFFLNVGSTPQSPWVALDVAQKLRGLFVLQNQIVWVKSIAVPGPDG
                     RTTAVGHYKPVNSRRFLHHCYEFIFHFTKTGEVPLDRLAIGVPYQDKSNIGRWRSATL
                     DRRCRGNTWFIPYKTIQSRADDRPHPATFPVELAEMCVRLHGRRAGMKVLDPFSGLGS
                     TARAAQRLGADFVGFEIDPAYVRQSRRLLTADRKK"
     gene            7224..7721
                     /locus_tag="P5079_05090"
     CDS             7224..7721
                     /locus_tag="P5079_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29398.1"
                     /translation="MIPHLDGATVLTAAQDAVKAVNQAVVPYLSRQIPLLDLKLQSNT
                     ICAVVHNLLARGISELNNAWQLREKGSAGGDLGGPENIEVEIKTTTDRRIKGNRIKLK
                     TGYFICLFLSKPAPEGLTLREVRCGYVQRQDWHVMPKTQFAYIKPEVIQMMDRVFPAT
                     ETAVP"
     gene            7739..8674
                     /gene="fabD"
                     /locus_tag="P5079_05095"
     CDS             7739..8674
                     /gene="fabD"
                     /locus_tag="P5079_05095"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRY29399.1"
                     /translation="MSIAFVFPGQGSQAVGMGKELADHFPAAKAVFDRAARVLGQEYV
                     DVIFSGPEEKLRQTKFTQPALFIVSMAALEVVKTSPLKPVLAAGHSLGEYSALCAAGA
                     FDFETGLNLVKRRGEALQEASDKIPGTMAAIVGLERAKVEEICAQAGAQGVCEAVNFN
                     CPGQIVLAGAGAAIEEAVRLAQAAGSPKSVRLNVSGPFHSSLMKPAAEKMAAVLEFAP
                     FNDAAIPVVANCDAQPTQKAEDIKAKLLKQIDHPVYWEETLRVMFAQGVDTFVEVGPG
                     RVLSGLLRRTDKTKKFRNIEDKKSADALLAPAVSS"
     gene            8738..9481
                     /gene="fabG"
                     /locus_tag="P5079_05100"
     CDS             8738..9481
                     /gene="fabG"
                     /locus_tag="P5079_05100"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRY29400.1"
                     /translation="MRLKDQAALITGGAQGIGRAIAETYAKEGCKISICDVNEPVAQA
                     TAEEIKKAYGVDTLAAKVNVTAIEECEKWVEATAQKFGRVDILVNNAGITKDNLVMRM
                     SDAEWDAVIAVNLKGVFNCTKAVVRPMFKARAGRIVNIASIVGLMGNAGQVNYSATKG
                     GVIAMTKTCAREFASRNVLVNAIAPGFIRTAMTDKLTDEQKKKLSEMIPLERLGEAQD
                     VANAALFLASAESSYITGQVISVNGGMYM"
     gene            9641..9880
                     /gene="acpP"
                     /locus_tag="P5079_05105"
     CDS             9641..9880
                     /gene="acpP"
                     /locus_tag="P5079_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY29401.1"
                     /translation="MADDIQQKVKDIIVEQLGVDAEQVKPEASFVNDLGADSLDTVEL
                     VMALEEAFDMEIPDEEAEKIQSVGQAVDYIKAHKK"
     gene            10053..11294
                     /gene="fabF"
                     /locus_tag="P5079_05110"
     CDS             10053..11294
                     /gene="fabF"
                     /locus_tag="P5079_05110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRY29402.1"
                     /translation="MKNRVVITGIGIVSPIGIGKERYWDALKNGRNGFGRISFFDPTV
                     YPCHIDAEVRDFQPEQFIEKKKARRMDRFTQFAMAGAKMAVEDARLDLTKLNLERCGV
                     IVGSGIGGLPTIEAEHSVLLEKGPKRVSPFLIPMLITNIAPGEIAIEYGFTGPNYSVS
                     SACATSNHALGEAFRHLRYGDADLLIAGGTEGAITPLGVAGFCQARALTISFNDNPAK
                     SSRPFDKNRSGFVMGEGCGVVIMETLEHAQARGAKIYAEFVGYGATDDAYHITAPNPE
                     GHAAVRAMQLALADGNVRPEEVDYVNAHGTSTELNDKTETAAIKKVFGDHARKLAISS
                     TKSMTGHLLGAAGGAELIATLLSMENKLIHPTINYETPDPECDLDYVPNTARPGEIRC
                     ALSNSLGFGGHNAVVVVKKFA"
     gene            11305..12024
                     /gene="rnc"
                     /locus_tag="P5079_05115"
     CDS             11305..12024
                     /gene="rnc"
                     /locus_tag="P5079_05115"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY29403.1"
                     /translation="MPRLETEVLERRIRLRFKNRKLIEEALTHKSFAIEKGAKAFNER
                     LEFLGDSVLSTVVAHYLFKRYPNDDEGRLSKLKSLLVSRPTLVVWAKELNLGDFLYLS
                     EGEEATGGRTRDSLLANVFEALLGAMFLDAGFQATQRFIVRRLSKKKRIVETDYKSKL
                     QEIIQKRYKIPPHYFVAKETGPDHDKTFLMEVRVRHRLLGQGTGRTKKEAEQAAAYQA
                     LKKIRSHRLAKKILADAGAAN"
     gene            12054..>12404
                     /locus_tag="P5079_05120"
     CDS             12054..>12404
                     /locus_tag="P5079_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY29404.1"
                     /translation="MNYFFTEDQQAIIETAREIAEKKIKPVREKYDREEHFPWDIVED
                     LRKADLFGVYIHPNYGGLGGGGFELVLVVEELSKVCGGTSLCLAATALGTFPIILFGN
                     PEQKKKYLPDIASGK"
BASE COUNT         2473 a         4114 c         3738 g         2079 t
ORIGIN      
        1 acggggctgt tggtggtgcc gctgcttttc gccggctggt ctttcaagat ttttcgcgcg
       61 gaccaatgtc tcgcgcaggc gaaaacggcg atggagacga acgacctcga tcgcgcctcg
      121 tcaaggatcg aggcgggtct gttctggaac ccccaggaag ccgaactgta ttccctgcgg
      181 agcgccttga aggcttttgc cggcgactgg gcggcggccg acgacaacgt ccgtaaaagc
      241 atccgcctct cgccgttcgt ctcccggttc catcgcgagg ccggagagat cgctcttcga
      301 cgcggccggc gggaggaagc cctgctttat tttgaaaccg cttcccggct tctcccgaga
      361 gacatcgcgc cctggctgag gatcgcgcag ctgcaggaag acgccggccg gctgccggcc
      421 gcggtggagg cctacgaacg cgtgttccgg ttcatggagg aaaaaccggc gagggacgcc
      481 ggctgggaaa cggaattgtc cgtcaaacta tcgaacctga aagagaaatt gacccatgcc
      541 ccgtaaaata aaggtcgccc acatcgtcac cttgctggag ttcggcggcg cgcagcaaaa
      601 caccctctac accgtcgaac acctggaccg caaccgtttc gaaccgctcc tcctctgcgg
      661 gcgcggcgcc tatctcgacg gggacgctga aaaaagcggc taccccacga tcttcatcaa
      721 cagcctggac cggcccctcc ggccgctgcg ggacatgctg gcctttctgg aaatattctt
      781 cgtactgaaa cgggaaaaac cggacatcgt ccacacccac tcctccaaag ccggggtcct
      841 cggccgctgg gcggccaaat gcgcgggggt accgttcatc gtccacacgt tccacgggtt
      901 cgggttcact tcaagacaga acatttggct ccgttggacg ttcatcctca ttgaaaagat
      961 atcggcctac gccgcccacg tcctcctcgc ggtctcccag gccaaccggg aggaggcgct
     1021 ccgtcacggc atcggccggc cggacaaata ccgtcttctc cgcagcggca tcgcccgccc
     1081 gcttttccag tccctctcgc gccggcccaa cgcccccgag gggcttccgc tcaccccgga
     1141 tttgcgcctc gtgacgacca tcgggccgtt caagccccag aaaaatctgt cggatttcgt
     1201 cgccatggcg gggcgtgtgc acgccaaaca cccggaagcg aggttcctga tcgtgggcga
     1261 cggggaactc cgccctctgt tggaacagga aatcgaacgg cacgggttga cggccgtttt
     1321 ttatcttccg ggctggcgga gggacatccc gcacattatg atgcgatcca acattttcgt
     1381 cctgacgtct ttgtgggagg ggctcccccg ggccctggtg gaagccatgt gcgccgcgct
     1441 cccctgcgtg gccaacgccg tggacggcgt caacgacatc gtcaacgacg gcgccaacgg
     1501 tttcctggtg ccgccccggc ggccggacct gaccgcggaa cgcgtgaacc accttctgga
     1561 gaaccccaac ttcgcctcgg agatgggcca tcgggcgcgt caatccatcg gcgacgaatt
     1621 cgacatcgac gagatggtcc gccgccagga agaactctac tccgccgctg tccatcctta
     1681 aactcgcccg ctccgttcct ccataaaaaa aataccgcgc gggcccgccc cccagggaga
     1741 gctccggcat gtcatttatg tttaggattt aacgggaaaa agcgaaacgg aacagcggat
     1801 tacttcgatt gggagcggcc ggccttgatc ttttcaaggc gcgcctggcg gttggcggcg
     1861 gagaatcgga gcgtccagga aaggcggtac atggtggtct tcacgtcgtc gtccccttcc
     1921 ttctgcttct tcatctgctg gatgatgctg cccacgctga ggttccagcg ggggatctgg
     1981 atgtccatgc cgtaggaggt gctgggcggg gacttttcgg cgtccgctga ggtggaggtg
     2041 aggtccgtgg tgacggtgaa actgccgccc aggttctgat tgagggtggc gaaggggaac
     2101 agccggaggt aattgttccg gtcgagcgga atcccgaccc cgatgcccgc ctgccagccg
     2161 tagagtaccc cgaaaatgtc cacggtgtcg tcctcggaca caatggtgcc gctggtgttg
     2221 gtgcgaagat agctggtgac gatggaggaa ttcatcatgg tgagcgtggg gccgacgctg
     2281 atggggagcg cccacccttt ttggttccgg gccggggtgc tgagcgggtc caccacgaag
     2341 ttgacccctt cgctcaacac cgaaaagccc atgtcgccct gaaaaccggc catgctcgaa
     2401 taattcgacg gcagatcgcc gcccattcct atgaaagcga agttgacggc cacgccggtg
     2461 tgcgtgctgg tgatggcgtt gaacacgccg gtatagccgc cgcccttcaa ggtgatgccc
     2521 ccggccttag ccttcagata gattaggtcg ttcgataatt ccgcccagtc ggtcaacagc
     2581 cgcatcatgg gaagcggcgg cggcaccacc tggaactgct gattttccgc ccggacgacc
     2641 ggaggcagtc ccgccaaaaa cagtccggcg cccatcatcg ccgctaaata tctcttattc
     2701 atttccgtat cccaaacggc tcaccgggtt gaacgccgtc ggcgcggccg cctccatggt
     2761 ggtcacgctg aacgtatatc gaaaccgcgt gctggaggat ccggtcccgg tgggcgtcac
     2821 caggacgttc atgcggacgc cggaatccgt tatcgtgccg cctcccacgc cggtgctgaa
     2881 gacgatgctg aaattgcctc ccgtgagcgt gcccgtgtag gggagccagg tcatgagggg
     2941 ggtggcgatg ttggtgatgg tgttcacggt gccgttggcg agggtgacgc cggtttggac
     3001 cgtgccgttc attttaatgg cggtgatgtc gcgggtgacg ttctttccgt tgctggtgtc
     3061 cctcacgcgc gtggtcttcc aggtggccgc tagaccggcg tttttcgaaa acggggaagc
     3121 ggtgcccatc accgcgagcg catcgccggc ggaatcttcg tagcggatct gctcggtggc
     3181 gttgctgtag actttgacgg tgtaataccc gctggaggcg tcgtaggcgg tgaaagcggc
     3241 ggcggaggcg atgtccacgt aataggtggc ccaggcgagt tcggagttgg cggaggcggc
     3301 ggcggaaacc gcggcgttgg ccacgctgcg ctgcacctcc aagtgggcgg ctttggcctg
     3361 cacctcggtt ttatgccggc cggtcgaaca accacacacg ccgaccagaa ggaaccccag
     3421 ggggaggata aaacgacgca tggagaaact ttaaaacacc cccgtttctt tgtcaagcgt
     3481 tttgcggggc ggcgctccgt tgacaccggt tccaaaattc aataaactca cgcctgtctt
     3541 tggaacggcc ctcgggccgg ttcccgcgga tctttcagtt ccggaggggg gacaacatga
     3601 aacttcgcgt ccacgaaatc ctggacgccg gtgtaatcga ccggaccgat accctgcccg
     3661 ccgccgacat acccttggat ttgccggaca accccgtctt gctggatccc gttgaaacca
     3721 aaatccacgc tgaaaaacta gaagaagaag tcctggtgtg gggggaggtt tcggcgaagg
     3781 tgcgcctgac gtgttcccgt tgtctggaac cgtacgagga agagccgctc ggcgggcggt
     3841 tcgaggttcg ggccccggcg cggcagaact tcgtggacgt gggcgaggag atccggcagg
     3901 ccctcctgct ggccatccct ccgaaacccc tgtgccgcgc gaactgcaag gggctgtgcc
     3961 cgaaatgcgg caagaatttg aaccagggcg tgtgcggctg tccttcccgg ccgggcgaat
     4021 cgcccttcgc caaactgcga gatttaaaaa tccaatagag aaaggctttc acgatggcaa
     4081 acccgaagaa aaaacatacc ccgatgcggc gcgacatgcg ccgcgcccag aactggcggt
     4141 tggaactcgg ctcgctctcc cgctgccctc actgcggcgg catgaggccg ccgcaccgcc
     4201 tctgccccgc ctgcggctac tacggcgccg agctcatcct tccgcccaaa gtcaaaaagg
     4261 ataagaaaga aaaatcctga ttaccggcgc cgaatttttc cgacggaaaa attcaaagat
     4321 gtccctccat ccatccgcgt gtccccctcg gaggcgttcg tgagcgtccg catcgccctg
     4381 gacgccatgg ggggagacct cggcctggcg gtgaacgtcg agggggccct tcaggccaaa
     4441 cgggaactcg gcgtggaggt caccctggtc ggcgaccagt cgctcctgga ggaggaactg
     4501 gaccggcaca acgcccgcgg ggaactggcc atcgtgcacg cccccacggt ggtgggcatg
     4561 cacgaaaaac cggccgaggc ctgccgcgcc aagaaagatt ccaccatcat gctctgcgcc
     4621 cagatggtcg ccgaaggccg ggcggacgcc tacgtctccg ccggcaactc cggcgccgcc
     4681 atgaccgccg ccctgtggca cctgcgccgc atccccggcg tgtcccggcc ggccatcgcc
     4741 accctctttc ccacgctcac cggctccacc gtcatcctgg acgtgggcgc caacgtggac
     4801 tgcaaaccca aacacctcgc ccagttcgcc ctcatgggca gcatcttcgc ccgctccgcc
     4861 ttcaacgtgg accagccccg cgtggggttg ctcaccatcg gcgaagagga aggcaagggg
     4921 aacctcctta cccaggagac ccacgagatg ctgaagaaaa atcctctcat caactacatc
     4981 ggccacgtcg aaggccgcga catcccggcg ggcgaggtgc acgtgttcgt ctgcgacggt
     5041 ttcgtgggca acgtggtgct caagttcggc gaagggctgg ccgcggcggt gatccacctc
     5101 atcaagacgg agatcaagaa acaccccctg gccatcttcg gccgatacct cctcaaaggc
     5161 gctttccgcg agatgaggaa acggaccgac ccgtcggaac acggcggcgc gccgctgctg
     5221 ggcgtcaacg gcgtggccat catcgcccac ggcggatcca acgccaaggc catcaagaac
     5281 gccctccgcg ccgcggcctt cttccacgaa aagggcgtca accggcagat ccacgaccag
     5341 atccatcaac tggacaccac ccagctcgtt ccgcagaaag cgaccgcctg accccatgac
     5401 cgggataaaa ttcctctcca ccggcgtcgg actgccggaa aacgtgctca ccaacgccga
     5461 cctggaaaaa atggtcgaga cctcggacca atggatcacc gagcgcaccg gcatcaaaac
     5521 gcgccgtatc gccagcgaca agcaagccac ctcggatttc tccatcgccg ccgcccggca
     5581 ggccctctcg cgcgccggca tcgccccgga aaccgtcgac gcggtggtgg tggccacctg
     5641 cacgcccgac catctcatgc cttcggtggc ctgcctcgtc caacgcgcgc tgggactcaa
     5701 gaacgccttc gccttcgacc tggaggccgc ctgctccggg ttcatcttcg gcctggcctg
     5761 cgtcaacggc ctcatgcgtt cgggcgcggc caagaccgtc cttctcatcg gcgccgacac
     5821 cctttccaaa ttcaccgact ggacagaccg cggtacctgc gtgctcttcg gcgacggcgc
     5881 cggcgcggcc ctcctgcagg cctccgaccg ggacgacaac ctcctcgccg tccacctcgg
     5941 gtccgacggc tccttcaccg acatcctgaa catccccggc ggcggatcgc gccatcccct
     6001 gcagcgcagc aagaacgccc cgttcaccac cggcgaagtc gcggggaact tcccgccgta
     6061 catccacatg gaggggaaag agatcttcaa aaacgcggtg ttgcgcatgg ccgaggcggc
     6121 ggacagcgcc ctgaagaagt gcggcaagac ctcggacgac ctgcgcctcc tcatcccgca
     6181 ccaggcgaat ttgcgcatca tcgacgccgt ggccaaacgg atgaaactgg gcgacgacaa
     6241 ggtttttcga aacgtgcaga agtacggcaa catgtcggcc gccaccaccg tcatcgccct
     6301 ggacgaggcc gtgcagggcg gcgtcgtcaa gcgcggggac ctggtggagc tcatcgcttt
     6361 cggcgggggc ctcacctggg gcgcggccgt cctgcgctgg tgaaatcgaa acacccgcgg
     6421 cgcccgccgc gcattgaact attccagcag gactgcgtcg ccggcatggc ccaacgcttg
     6481 aaggacggcg aaatcgacgt cgtcgtcacc tccccgccct acaacctcgg catccgctac
     6541 acccgctacc gcgacaaccg tccctatcac gactatctga gctggctgga atgcgtggcg
     6601 cgcggcatca aacgcgtgat gcgtcgcgac ggttctttct ttctgaacgt gggcagcacc
     6661 ccgcagagcc cctgggtggc tctggacgtg gcccagaaac tccgcggcct cttcgtcctg
     6721 cagaatcaga tcgtgtgggt gaaatccatc gccgtgcccg gccccgacgg ccgaaccacc
     6781 gccgtgggcc attacaaacc cgtcaacagc cggcggttcc tgcaccactg ctacgaattc
     6841 atcttccact tcaccaagac cggcgaggtg ccgctggacc ggctggccat cggcgtgccg
     6901 tatcaggaca agagcaacat cggccgatgg cggtccgcca cgctcgaccg ccgctgccgg
     6961 ggaaacacct ggttcatccc gtacaaaacc atccagtcgc gggcggacga ccgcccccac
     7021 ccggccacct tcccggtgga actggccgag atgtgcgtgc ggctgcacgg ccggcgcgcc
     7081 ggaatgaaag tgctcgaccc tttttcgggg ctcggttcca ccgcccgggc ggcccagcgg
     7141 ctgggcgcgg atttcgtcgg attcgagatc gatccggcct acgtacgcca gtcccggcgg
     7201 ctgttgacgg cggacagaaa aaaatgatcc cgcacctgga cggggcgacg gtcctgaccg
     7261 ccgcccagga cgcggtgaaa gccgtcaacc aagcggtggt gccttacctc tcgcgccaga
     7321 tcccgctcct ggacctgaaa cttcagtcca acaccatctg cgccgtggtt cacaatttgc
     7381 tggcgcgggg catctccgaa ctgaacaacg cctggcagct gcgggaaaag ggttccgccg
     7441 gcggggattt gggcgggccg gagaacatcg aggtggagat caagaccacc accgaccgcc
     7501 gcatcaaggg caaccgcatc aagctgaaga cgggctactt catctgcctg ttcctgtcca
     7561 agcccgcgcc ggaagggttg accctgcgcg aggtgcgctg cgggtacgtg cagcggcagg
     7621 actggcacgt gatgcccaag acccaattcg cctacatcaa gcccgaggtt atccagatga
     7681 tggaccgggt gtttcccgca acggaaaccg ctgtccctta ataaggaggg gaatccacat
     7741 gagcatcgcg ttcgtttttc caggacaagg gtcgcaggcc gtcggcatgg gcaaggaact
     7801 ggccgaccat tttcccgccg ccaaagcggt gttcgaccgg gccgcccggg tgttgggaca
     7861 ggagtatgtg gacgtcatct tctccggccc ggaggaaaaa ctgcggcaga cgaagttcac
     7921 ccagccggcg ctcttcatcg tgagcatggc ggccttggaa gtcgtcaaga cctcgccgct
     7981 caaacccgtc ttggccgccg ggcacagtct cggcgaatat tcggccctct gcgccgccgg
     8041 cgccttcgat ttcgagaccg gcctgaacct ggtgaaacgg cgcggggaag ccctgcagga
     8101 ggcctccgac aaaatccccg ggaccatggc cgccattgtc ggtctggaac gcgccaaggt
     8161 ggaggagatc tgcgcccagg ccggcgccca gggcgtatgc gaggcggtga acttcaactg
     8221 cccgggccag atcgtcctgg ccggcgcggg cgcggccatc gaggaggcgg tgcgtctggc
     8281 ccaggccgcc ggctcgccca aatccgtgcg gctcaacgtc tccggcccgt tccattcctc
     8341 gctgatgaaa cccgcggccg agaaaatggc cgcggtgctg gaattcgccc cgttcaacga
     8401 cgcggcgatc ccggtggtgg ccaactgcga cgcccaaccc acgcaaaaag ccgaggacat
     8461 caaggcgaaa ctgctcaaac agatcgacca tcccgtctac tgggaggaga cgcttcgcgt
     8521 catgttcgcg caaggcgttg acaccttcgt ggaggtgggg ccgggccggg tcctgagcgg
     8581 attactccga cgcaccgaca aaaccaagaa gttccggaac attgaggaca aaaaaagcgc
     8641 cgacgccctt ttggcccctg ccgtttcatc ctgaaatctg ctagaatacg ctgattttat
     8701 tggacgcttt gcaaccttat aaggagaccg ttcggacatg cgactgaaag atcaagcggc
     8761 actcatcacg ggcggcgccc agggcatcgg ccgggccatc gccgaaacct acgccaagga
     8821 aggctgtaaa atttccatct gcgacgtgaa cgaacccgtg gcccaagcca ccgcggaaga
     8881 gatcaagaag gcctacggcg tggacaccct cgccgccaag gtcaacgtga ccgccatcga
     8941 ggaatgcgag aaatgggtgg aggccaccgc gcaaaagttc ggacgggtgg acattctggt
     9001 gaacaacgcc ggcatcacca aggacaacct ggtgatgcgc atgagcgacg cggagtggga
     9061 cgcggtcatc gccgtgaacc tcaaaggcgt gttcaactgc accaaggccg tggtgcggcc
     9121 catgttcaag gcccgcgccg ggcgaatcgt caacatcgcc tccatcgtgg ggctgatggg
     9181 caacgccggg caggtcaact actcggccac caagggcggc gtgatcgcca tgaccaaaac
     9241 ctgcgcccgc gagttcgcca gccgcaacgt gctggtcaac gccatcgcgc ccggcttcat
     9301 ccgcaccgcc atgacggaca agctgaccga cgagcagaag aaaaaacttt cggagatgat
     9361 cccgctggaa cggctcggcg aggcgcagga cgtggccaac gccgcgctct tcctggcctc
     9421 cgcggaaagt tcctacatca ccggccaggt gatctccgtc aacggcggga tgtacatgta
     9481 gccccggggg cccggcggcc ccctgttccg attttcaacg ccggaaacct cggcgaaaaa
     9541 aacaaatggt gaaaagatca aggagtcttc cggcggggcg ccgttccggc cccacccccg
     9601 cgaaacgcct tcatcgtttc accacaccag gaggagtcac gtggcagacg acattcaaca
     9661 aaaagtgaaa gacatcatcg tggaacagct gggtgtggat gcggagcagg tcaaaccgga
     9721 agcgtccttc gtcaacgacc tcggcgccga ttccctcgac accgtggagc tggtcatggc
     9781 cctggaagaa gcgttcgaca tggaaatccc cgacgaggaa gcggaaaaga tccaatccgt
     9841 cggacaggcc gtcgactaca tcaaggccca caagaaatag ctctcgcgtc cggaccccgt
     9901 cgcggggttc ggtccgttcc cgtttcatcg taaaccgccc ggaggacggg gggactcctt
     9961 catagggatg cccccgtccc gggcggggaa cggccgccgc cgcgccgccg gacatctcgc
    10021 ttattttccg acaaacccca ggagaccctt tcgtgaagaa ccgcgtcgtc atcaccggca
    10081 tcggcatcgt cagccccatc ggcatcggca aggaacgtta ttgggacgct ctcaagaacg
    10141 gccgcaacgg tttcggccgc atcagctttt tcgaccccac cgtctatccc tgccacatcg
    10201 acgcggaggt ccgcgatttt cagccggaac agttcatcga aaagaagaag gcccggcgga
    10261 tggaccgctt cacccagttc gccatggccg gcgccaagat ggccgtggag gacgcccgac
    10321 tcgacctcac caagctgaac ctggaacgct gcggcgtcat cgtcggctcc ggcatcggcg
    10381 gcctgccgac catcgaggcc gaacattccg tcctcctgga aaaaggcccc aaacgcgtct
    10441 ccccgttcct catccccatg ctcatcacca acatcgcccc gggagagatc gccatcgaat
    10501 acgggttcac cgggcccaac tattcggtct cctcggcctg cgccacctcc aaccacgcgt
    10561 tgggggaggc gttccgtcat ctgcgctacg gcgacgccga ccttctgatc gccggcggca
    10621 ccgaaggcgc catcaccccg ctgggcgtgg ccgggttctg ccaggcccgc gcgctcacca
    10681 tctccttcaa cgacaacccc gccaaatcca gccgcccctt cgacaagaac cgctccggtt
    10741 tcgtcatggg cgaaggctgc ggcgtggtga tcatggaaac cctcgagcac gcccaggccc
    10801 gcggcgcgaa gatctacgcc gagttcgtgg gatacggcgc caccgacgac gcctaccaca
    10861 tcaccgcccc gaacccggaa ggccacgccg cggtgcgcgc catgcagctg gctctggccg
    10921 acggcaacgt gcggccggag gaggtggact acgtcaacgc ccacggcacc tccaccgaac
    10981 tgaacgacaa gaccgagacc gcggccatca aaaaagtctt cggcgaccac gcccgaaaac
    11041 tcgccatctc ttccaccaaa tccatgaccg gccacctgct gggggccgcc ggaggggcgg
    11101 aactcatcgc caccctcctc agcatggaga acaaactcat ccaccccacc atcaactacg
    11161 aaacccccga cccggaatgc gacctggact acgtgcccaa caccgcccgg ccgggggaga
    11221 tccgctgcgc gctctccaac tccctcgggt tcggcgggca caacgccgtc gtcgtggtca
    11281 agaagttcgc ctaacggccc ccctatgcca cgactggaaa cggaagtcct cgagcgccgg
    11341 atacgccttc ggttcaagaa ccgcaaactg atcgaggagg cgcttaccca caaatccttc
    11401 gccatcgaga agggcgccaa agcgttcaac gaacgcctgg aatttttggg ggacagcgtg
    11461 ctctccaccg tggtggcgca ttatcttttc aagcgttatc cgaacgacga cgagggccgg
    11521 ctgtcgaagc tgaagtccct tctggtgtcg cggccgacgc tggtggtgtg ggccaaggag
    11581 ttgaacctgg gggatttcct ctatttgagc gagggggagg aagccaccgg cggccgcacg
    11641 cgcgacagcc tgctggccaa cgttttcgag gcgctcctgg gcgccatgtt tctcgacgcc
    11701 gggttccagg cgacccagcg gttcatcgtg cggcgcctgt cgaagaagaa acgcatcgtc
    11761 gagacggact acaagagcaa actgcaggag atcatccaga aacgctacaa gatcccgccg
    11821 cactatttcg tggccaagga gacgggcccc gaccacgaca agaccttcct gatggaggtg
    11881 cgcgtccggc accgcctcct ggggcagggc accggccgca ccaagaagga ggcggaacag
    11941 gccgccgcct atcaggccct gaagaagatc cgcagccacc ggctggccaa gaagatcctg
    12001 gcggacgccg gcgccgccaa ctgaactgtt ttcaaacaca cggaggtaac cccatgaatt
    12061 atttcttcac cgaggaccag caagccatca tcgaaacggc ccgcgagatc gcggagaaaa
    12121 agatcaagcc cgtgcgtgaa aaatacgacc gcgaggaaca tttcccctgg gacatcgtcg
    12181 aggacctccg caaagcggac cttttcggcg tctacatcca ccccaattac ggcgggctgg
    12241 gcggcggcgg gttcgagctg gtgctggtcg tcgaagagct ttccaaggtc tgcgggggga
    12301 cgtccctctg cctggcggcc acggccctcg gcaccttccc catcatcctc ttcggcaacc
    12361 cggaacaaaa gaaaaaatat ctccccgaca tcgcctccgg caag
//
LOCUS       DAOTFD010000058        12314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3607_length_12314_cov_4.550861, whole genome shotgun sequence.
ACCESSION   DAOTFD010000058 DAOTFD010000000
VERSION     DAOTFD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_12314_cov_4.550861"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="P5079_05125"
     CDS             <1..885
                     /locus_tag="P5079_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY29405.1"
                     /translation="VVVERAGEVIPKVIKVLSEKRTGDERSVRPPEKCPACGGRIDKG
                     KEEDVIYRCLNPACPAQLEKSLLHFAGREALDIEGMGEAVAQELHTRKLVSDLADVFT
                     LKKSDLLGLEGFKDKKADNLLHAIEAAKSRSLDRFIYSLGIRDVGEKGALLLAERFGT
                     LEKLRNATAEELTAIHEVGPVMSESVVNFFRQPSVKKLLDKFKRLGVEPRPVPRTERP
                     ATLAGKTVVFTGELRSFSRAEAERLTRELGGNAVSSVSKNTSFVVAGEDAGSKFRKAQ
                     KIGVEIIDELEFRKRAGL"
     gene            915..1424
                     /locus_tag="P5079_05130"
     CDS             915..1424
                     /locus_tag="P5079_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRY29406.1"
                     /translation="MSSDFSTPPSSFKKPDNRCLKTASRLLKLRDRSRTEIEKLLLQR
                     GFGTEEVRETLRRLEEAGLLDDERAARFWIDKGRRAGHGTLRLRETLAQRDIPEPLIA
                     KALGGDERRDEVARARTVLERQSRRMGKLSPAVRARRLAAHLTRRGFSAECIEQLLER
                     FNTEPTESE"
     gene            1434..2990
                     /gene="purH"
                     /locus_tag="P5079_05135"
     CDS             1434..2990
                     /gene="purH"
                     /locus_tag="P5079_05135"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRY29407.1"
                     /translation="MSDLKRALLSVSDKTGLVELAHGLSEMGVEILSTGGTAKTLRQA
                     EIFAKEVSEVTGFPEILDGRVKTLHPKIFGGILAKREKKSHQQDAANHGIGPIDLVVV
                     NLYPFETACGDERLGEEDLLEYIDIGGVSLLRAAAKNHEDVAPVCDPSDYPAVLKELS
                     ESGRLGPDTRRRLAAKAFAHTAHYDSVIAAHFRQKLGAREYPTELSVGLRKQLDLRYG
                     ENPHQKAAFYQESGMKPWGVGGAKILQGKAISFNNYLDCDAAWRLVCSFQNPACVVVK
                     HNNPCGACEGETPADTFRRAYAADSLSAFGGIVSFNRAVDGETAQELSKYFLECVMAP
                     SFHDEAKSVLSKKQNLRLLEQPALLADPYEWDVRKISGGYLVQDLDTNPAAEQRVVSQ
                     RAPTAEEKLSLEFAWKVAKFVKSNAIVLARGRQTVGVGAGQMSRVYSLKIAVMKMQSQ
                     QGLAVASQPLVLASDGFFPFRDTLDEAAKIGVTAVIQPGGSVRDEESVTAANEHNMAM
                     LVTGVRHFRH"
     gene            3115..3420
                     /locus_tag="P5079_05140"
     CDS             3115..3420
                     /locus_tag="P5079_05140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRY29408.1"
                     /translation="MPEKKHQGWKDLVHGGLLAALLDEAMTRLVWQRVGAALTAEMTV
                     RYLAPALVGEKLKIRGEVLDDSRRLVLTRAEILKSDGTVVARAEGKTLKASARKKEG"
     gene            3422..6067
                     /gene="alaS"
                     /locus_tag="P5079_05145"
     CDS             3422..6067
                     /gene="alaS"
                     /locus_tag="P5079_05145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY29409.1"
                     /translation="MLTGNRIRESFLAYFEGGDRRHKRMPSDSLIPSSDPTLLFTSAG
                     MVQFKPYFLGQRVLPGNRAASSQKCLRTTDIERVGHTARHLTFFEMLGNFSFGDYFKK
                     DAVAWGWEFLVKEMKLPADRLYSTVFREDDEAFDLWKKLQPESKIVRMGEDTNFWNMG
                     PTGPCGPCSEILLDKGPEFCTCGAREKCRPENDCDRWMEIWNLVFTQFDRQENGTLTP
                     LPKKNIDTGMGLERLTAAVQGVSSNFETDIFQSLIRLTEEILGRSSHKKAGPERAPFR
                     LIPDHARAAAFMIADGILPSNEGRGYVLRRLIRRAVRHGRLAGRRDPFLSRLTGEVAE
                     QMREVYPDLRQKRENIAAIVKQEEERFFETLESGTERLEELLRAAKKSKQTRLSGVEV
                     FRLYDTYGFPPDMTREILQEQHMTYDEKEFEDAKTRAQDVARDAWKGSGQEDVQVYNE
                     LLKNIGPTRRAFYKDYGAGETEATVVALLQDQTRVKELPEGATGEIVLSDTPFYPEGG
                     GPVGDTGVLKVGKEIVAEVLDTQKPVEGLIVHRVRVKKALKESARLLAQVDMERRRAI
                     MRHHTATHLLHAALRRVLGTHVTQAGSIVTPDKLRFDYSHPGTPSAEELRLIEQDANK
                     SVLADIRHERKETSLAEAQAMGAMAFFGEKYGEKVFVVKFGGASTEVCGGLHTATTGE
                     VGLIKLTSESSIGAGVRRIEAVAGLKTLDYLNEMETALGQVSEKLKAPPKDLAVKIDK
                     LFQAQRQQEKEIGELRLRLAQGDGGGLAAPEAKDVSGIKCLLKAAAGMDEKSLRTLTD
                     RLRTKLPSGLVITATQNEEKTSFVVSFSEDLVKQGWHAGKVAQSIARKIEGRGGGRAD
                     FAQGGGKNALPLEKLFADLSQFVQK"
     gene            6094..6167
                     /locus_tag="P5079_05150"
     tRNA            6094..6167
                     /locus_tag="P5079_05150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6128..6130,aa:Arg,seq:ccg)
     gene            6223..6624
                     /locus_tag="P5079_05155"
     CDS             6223..6624
                     /locus_tag="P5079_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29410.1"
                     /translation="MRKKKSARPPVIGVDFDGTLCQIFKSNVPYIPKSVIRGKPNKNV
                     VEACRRYRRSGCRIVVYTSRWWGDYHWLKQWLDKYDVPYDDIVPGKFKADAFIDDTTV
                     NPSLDRDWEDRLCTILGKDLPRGKCPEGQKS"
     gene            6626..7075
                     /locus_tag="P5079_05160"
     CDS             6626..7075
                     /locus_tag="P5079_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004598189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HRY29411.1"
                     /translation="MIIAVIGQSSCPDSLAKTAESVGAEIARRGHVLVCGGMGGVMEA
                     ACRGAKRAGGVTLGILPGKDKKDANPYVDYPVLTAMSHARNAVIVRTADAVIAVGGRY
                     GTLSEIGLALSIDRPVYGLKTWSIRGVSPVKTPKEALDRIEARFKKS"
     gene            7104..7598
                     /gene="dtd"
                     /locus_tag="P5079_05165"
     CDS             7104..7598
                     /gene="dtd"
                     /locus_tag="P5079_05165"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRY29412.1"
                     /translation="MKALIQRVSAAGVSIENQPERPIGRGLVVFLGITASDTDKDIRW
                     LAEKILNLRVFPKPPAEAAAAPSDSEFDLAVADIGGEILLVSQFTLYADVRRGRRPDF
                     SRAAPPAQAQILYEKFVTRLRGLHPKLVTGEFGARMRVRIQNEGPVTLLIESPADAPA
                     VETA"
     gene            7595..9631
                     /locus_tag="P5079_05170"
     CDS             7595..9631
                     /locus_tag="P5079_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29413.1"
                     /translation="MTSPPPLQKFLDAFKKRAWLLKPLSPLARLDLRVWALVLILCCG
                     VSGVLFWFLRRPANVLFIEPLRAEFYHDQFLEWTLRAQPAALDWKLRKMKFNTWLERS
                     GERVVSVGDIRETVLRYDKKAGLWRARIPCPWNAPAGDYAIRVDTSALPVESLRLRAR
                     PLRVADRAFKPLPPGFGVLTLESIGPLRKLLAPDGTRKDGTALAEWAEFIGADAVVIQ
                     GAESGGYGKKLPAEFPWITQSKAAIKTVARECHKRGLKFGVYVMSYMVGGTPDKSPDY
                     DYGWIYEDGRMINGLEMRKRRGVSITDPRRIKDIINVARPWYDMEEVDIVGLDYIRPV
                     FGGYELVDAFVRDMPVTPPADWDKRSLQQRMTWLFTHRSAGGHNFIEQWYWYRAHRTA
                     QVVRAIKAGLGDKKPLWAFTLSWEKGWHHGQDPVMMRDAGIDIDAIMAYEADSRQFQG
                     FVKQWREYVRRKEVNLLVGDAVDWVLHQRTLNPAGPEDFYNRDILAVSQFHADGPVRG
                     VFVHDYVRATRGRLGPYSTLEWFLAGGAIITTTCRLNGALGYDLTLSLPDRAAAGETV
                     TGKLTLGRKRPAGTIRAHLYSAADVSMPDRDFELSPSTPAVAFTFRWVPDHTSAPRGH
                     RSFVAVRAERTAPSAERCQIHIMYFQGQRGNKTPEKPAPKNEGATENRDETKPK"
     gene            9645..10343
                     /locus_tag="P5079_05175"
     CDS             9645..10343
                     /locus_tag="P5079_05175"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRY29414.1"
                     /translation="MSFVLAMLLSNAARLFAGAPPKTDPAADRRNEMVRSQIERRGVK
                     DARVLNALRRIPRHLFVPEEMRSSSYDDEALPIGAGQTISQPYVVAAMTERLRLTGEE
                     RVLEIGTGSGYQAAVLAELAAQVYSVEIIPELADRARKTLSSLNYSNVHVRTGDGGKG
                     WPEKAPFDAVIVTAAPEDVPPALLEQLKMGGRLVIPVGPRENQDLVCFHKTPDGVRKE
                     ILFPVRFVPFVRGE"
     gene            10340..10753
                     /locus_tag="P5079_05180"
     CDS             10340..10753
                     /locus_tag="P5079_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29415.1"
                     /translation="MKKSLLMLLLLTAGGVTVRAEPPDPFASFEIPSSTKPASAEMSP
                     FVKELATTLNWPADQLQTLERRGFGRTEMATFVAIAKKTSKPWAELIKAREKGATLRQ
                     MTEEAGLVYNDVFRESRRAKQNVENRLKNTAASKQ"
     gene            10963..12270
                     /locus_tag="P5079_05185"
     CDS             10963..12270
                     /locus_tag="P5079_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY29416.1"
                     /translation="MKNFVLDTNVVLYDPNAIFSFEDNNVIIPIWVVEEIDRFKKDIN
                     DTGRNARELSRHLDALRKKGDITQGVPLESGGQLQVITSIPLSPDAPEFLKKESYDNL
                     ILATAFYLKKKNPPVIFITKDVNLRIKADALGIDTMDYETMRIEPEGLYTGWKSLTVP
                     ESLISDFYQSKKLDTAKLPKNNFHENEFVCLQGETNASQSALGIYRKGEVQLLPPLPP
                     AVWGVKPRNLEQQFALHLLLDDRIPLVTLTGKAGTGKTLLALAAGMAKTLDEGIYQRV
                     LVSRPIFPIGRDIGYLPGDVKEKLQPWMQPIFDNMEFLLHTAKNDKKGPRYNALIEQG
                     LLQIEALTYIRGRSIPNQYFIVDEAQNMTPHEAKTVITRVGEHTKIIFTGDIHQIDNF
                     YNDASHNGLSYIVEHLKAEGLAGHMTLTKGERSALAELASNLL"
BASE COUNT         2566 a         4118 c         3672 g         1958 t
ORIGIN      
        1 gtggtggtgg aacgcgccgg cgaggtgatc cccaaagtga tcaaggtcct ctccgaaaaa
       61 cgcaccggcg acgaacgctc cgtgcgcccg ccggaaaaat gcccggcctg cggcggccgg
      121 atcgacaagg gcaaggagga agacgtcatc taccggtgcc tgaaccccgc ctgccccgcg
      181 caactggaaa aaagccttct gcatttcgcc gggcgggagg cgctggacat cgaagggatg
      241 ggggaggccg tggcgcagga acttcacaca cggaaactgg tgtccgacct ggccgacgtc
      301 ttcaccttga agaaatccga cctcctcgga ttggaggggt tcaaggacaa aaaagccgac
      361 aacctcctcc acgccatcga ggcggccaag tcccgttccc tggaccgttt catctattcc
      421 ctcggcatcc gggacgtggg ggaaaaaggc gccctgctcc tagccgaacg gttcggcact
      481 ttggagaaac tccgcaacgc gacggcggag gagctgaccg ccatccacga ggtggggccg
      541 gtgatgtcgg aatcggtggt gaattttttc cgccagccct cggtgaaaaa actcttggac
      601 aagttcaagc ggttgggcgt ggaaccgcgg cccgtgcccc gaaccgaacg gcccgccaca
      661 ctggccggca aaaccgtggt gttcaccggg gaactccgct ccttctcccg tgccgaggcg
      721 gaacgcctca cccgcgaact gggcggcaac gccgtctcct cggtgtccaa gaacacctcg
      781 ttcgtcgtgg ctggggagga cgccggttcc aagttccgga aggcgcagaa aatcggcgtg
      841 gagatcatcg acgagctcga attcaggaaa cgggccggtt tgtgacccca aaaaccgcgg
      901 atatttagca taatatgtcg tccgattttt cgaccccgcc ctcgtccttc aaaaaaccgg
      961 ataaccggtg tctgaaaacc gcgtcgcgtc tcctcaaatt gcgcgaccgc agccggacgg
     1021 aaatcgaaaa gctccttttg cagaggggtt tcggaacgga ggaggtgcgg gagaccctcc
     1081 gccgcctgga agaggccggc ctcctcgacg acgagcgcgc cgcccggttt tggatcgata
     1141 agggccgccg cgcgggacac ggcacgctcc gcctgcgcga gacgctcgcc caacgggaca
     1201 tcccggaacc cctcatcgcc aaagcgctgg gcggcgacga acggcgcgac gaggtcgcgc
     1261 gggcccggac ggttctggaa cgtcaatccc gccggatggg aaaactgtcc ccggcggtcc
     1321 gggcacgccg cctggccgcc catctcaccc ggcgcgggtt ttcagcggaa tgcatcgaac
     1381 agctgctcga acgatttaac acggaaccga cagaaagcga gtgaccgtca aaaatgagcg
     1441 acctgaaacg cgccctcctc tccgtctcgg acaagaccgg cctggtcgaa ctggcccacg
     1501 gcctctcgga gatgggcgtg gagatccttt ccaccggcgg caccgccaag accctgcgcc
     1561 aagcggagat tttcgccaag gaagtctccg aggtcaccgg ttttccggag atcctcgacg
     1621 gccgcgttaa aacgctgcat cccaagatct tcggcggcat cctggccaaa cgggagaaaa
     1681 agtcccacca acaagacgcc gccaaccacg gcatcggccc catcgacttg gtggtggtga
     1741 acctctatcc tttcgagacc gcctgcggcg acgaacgcct gggagaggag gacctgctgg
     1801 aatacatcga catcggcggg gtttccctgc tccgcgccgc ggccaagaac cacgaggacg
     1861 tcgcgccggt gtgcgatccg tccgactacc ccgcggtgtt gaaggaactt tccgaatcgg
     1921 gccggctggg tcccgacacc cgccgacggc tggcggccaa ggccttcgcc cacaccgccc
     1981 attacgactc cgtcatcgcc gcccatttcc gccagaaact cggcgcgcgc gaatatccga
     2041 cggaactctc cgtggggctg cgcaaacaac tggacctgcg ctacggcgag aacccccatc
     2101 aaaaggccgc cttctaccag gaatcgggca tgaaaccctg gggggtgggc ggggcgaaga
     2161 tactgcaggg gaaagccatc tccttcaaca actatctgga ctgcgacgcc gcctggcggc
     2221 tggtctgctc gttccaaaac ccggcctgcg tcgtcgtcaa acacaacaac ccctgcggcg
     2281 cgtgcgaggg cgaaaccccg gcggacacgt tccggcgggc ctacgccgcg gacagcctct
     2341 cggccttcgg cggcatcgtg agcttcaacc gcgccgtgga cggggaaacc gcgcaggaac
     2401 tgtccaaata tttcctcgaa tgtgtgatgg cgccctcgtt ccacgacgag gccaaaagcg
     2461 tcctgtccaa gaaacagaac ctgcgcctgt tggaacaacc cgccctcttg gccgacccct
     2521 acgagtggga cgtgcgcaag atctccggcg gctatctggt gcaagatttg gacaccaacc
     2581 ccgcggccga acaacgggtg gtctcccaac gagcccccac ggcggaggag aaactgtccc
     2641 tggagttcgc ctggaaagtg gccaagttcg tgaaatccaa cgccatcgtg ctggcgcggg
     2701 gccggcagac ggtcggcgtg ggcgccggac agatgtcgcg ggtctattcg ctgaagatcg
     2761 cggtgatgaa gatgcagtcc cagcagggat tggccgtcgc ttcccaaccg ctggtgctgg
     2821 cctccgacgg ttttttcccg ttccgcgaca cgttggacga ggccgccaag atcggcgtga
     2881 cggcggtgat ccagcccggc ggcagcgtgc gcgacgagga aagcgtcacg gcggccaacg
     2941 aacacaacat ggccatgctg gtgacggggg tgcgtcattt ccgccattga aaaaacgcct
     3001 tcggtcctgc cggacctgga ggacgacggc cactgtttcg tctgcggcga acgcaacgcc
     3061 gacggactcc gccttcgctg gacggtgcgg gggaaagaga ccttcgccga gttcgtgccg
     3121 gagaaaaaac accaaggctg gaaagacctg gtccacggcg ggctcctggc cgcgctgttg
     3181 gacgaagcca tgacccggct cgtctggcaa cgggtggggg cggcgttgac ggccgaaatg
     3241 acggtccgtt acctggctcc ggccctcgtg ggggaaaagc tcaagatccg cggcgaggta
     3301 ttggacgatt cccggcggct ggtgctgacc cgggcggaaa tcctgaaatc cgacggtacc
     3361 gtggtggcgc gggcggaagg caaaacgctg aaggcctccg cgcggaaaaa agagggatga
     3421 catgctgaca ggcaaccgga tccgcgaaag tttcctcgcc tatttcgaag gcggcgaccg
     3481 ccgccacaaa cgcatgccgt cggactccct catcccctcc agcgacccca ccctgctgtt
     3541 cacctccgcc ggcatggtgc agttcaaacc gtatttcctc gggcagcgcg tcttgccggg
     3601 caaccgcgcc gcctcctcgc agaaatgtct gcgcaccacc gacatcgaac gggtcggcca
     3661 caccgcccgc cacctcacct tcttcgagat gctcggcaat ttctccttcg gcgattattt
     3721 caagaaggac gcggtggcct ggggctggga gtttttggtg aaagagatga aacttcccgc
     3781 cgatcggctc tattccacgg tgttccgcga ggacgacgag gcgttcgacc tctggaaaaa
     3841 actgcagccg gaatccaaga tcgtccgcat gggagaagac accaatttct ggaacatggg
     3901 ccccaccggc ccctgcggcc cctgttcgga gatactgctc gacaaggggc cggagttctg
     3961 cacctgcggc gcgcgggaaa aatgccgccc ggaaaacgac tgtgaccgct ggatggagat
     4021 ttggaacctg gtgttcaccc agttcgaccg ccaggaaaac gggaccctca cgcccttgcc
     4081 gaagaaaaac atcgacacgg ggatgggact ggaacgcctc accgccgccg ttcagggcgt
     4141 gtccagcaat ttcgagacgg atattttcca atcgctcatc cgcctcaccg aggaaatact
     4201 gggccggtcc tcgcacaaga aggccggtcc ggaacgggcg cctttccgcc tgatccccga
     4261 ccacgcccgc gccgccgcct tcatgatcgc ggacggcatc cttccttcca acgaaggccg
     4321 gggctacgtc ctgcggcggc tcatccgccg ggcggtgcgc cacggccggc tggcgggccg
     4381 gcgggacccg ttcctctccc gtctcaccgg cgaggtggcg gaacagatgc gggaagtcta
     4441 tcccgacctg cggcagaaac gggagaacat cgccgccatc gtcaagcaag aggaagaacg
     4501 cttcttcgaa acactggaga gcggcaccga gcgcctggaa gaacttctcc gggcggcgaa
     4561 aaaatccaaa cagacgcgtc tctccggcgt ggaggtgttc cgtctctacg acacctacgg
     4621 cttcccgccc gacatgacgc gcgagatcct tcaggaacaa cacatgactt acgacgaaaa
     4681 agaatttgaa gacgccaaga cccgcgccca ggacgtggcc cgcgacgcct ggaaaggatc
     4741 gggccaggag gacgtgcagg tctacaatga acttttgaaa aacatcggac cgacccgccg
     4801 cgcgttctac aaagactacg gcgccggcga gacggaggcc acggtcgtgg ccctccttca
     4861 ggaccaaacc cgggtcaagg agcttccgga aggagccacg ggagagatcg ttctgagcga
     4921 cacccctttc tatcccgaag gcggcggacc ggtgggagac accggcgttt tgaaagtcgg
     4981 gaaggagatc gtcgcggagg tgttggacac gcaaaaaccc gtggaagggc tcatcgtcca
     5041 ccgcgtgcgg gtgaaaaaag ccctgaagga gtcggcccgc ctcctcgccc aagtggacat
     5101 ggaacggcgt cgggccatca tgcggcacca caccgccacc catcttctgc acgccgccct
     5161 ccggcgggtc ctgggcacgc acgtcaccca ggccggctcc atcgtcaccc ccgacaagct
     5221 ccggttcgac tactcccacc ccggcacgcc gagcgccgag gaactgcgcc tcatcgagca
     5281 ggacgcgaac aagagcgtgc tggccgacat ccgccacgaa cggaaggaaa cctccctcgc
     5341 ggaggcccag gccatgggcg ccatggcgtt cttcggggag aaatacgggg aaaaggtgtt
     5401 cgtcgtcaaa ttcggcggcg ccagcaccga ggtctgcggc ggcctccaca ccgccaccac
     5461 cggggaggtc gggctcatca agctcacctc cgaatcctcc atcggcgccg gcgtacggcg
     5521 catcgaggcg gtggccggcc tcaaaacact cgactatctg aacgagatgg aaaccgcgct
     5581 gggccaggtg tccgagaaac tcaaggcccc gccgaaagac ctggccgtca agatagacaa
     5641 actcttccag gcgcagcgcc aacaggaaaa agagatcggc gagctccgcc tccgattggc
     5701 ccagggagac ggcggcgggt tggccgcccc ggaagccaaa gacgtctccg ggataaaatg
     5761 cctgctcaaa gccgcggccg gcatggatga gaaatccctc cgcaccctga cggaccgtct
     5821 ccgcacgaaa ctccccagcg ggctcgtcat caccgccacc cagaacgaag agaagacgtc
     5881 gttcgtggtc tctttcagcg aggacctggt caaacaaggg tggcacgcgg gaaaggtcgc
     5941 ccaatccatc gcccggaaga tcgagggccg cggcggcggc cgggcggatt tcgcccaggg
     6001 cggggggaaa aacgcgctgc ccctggaaaa gctcttcgcc gatttgtcgc agttcgtcca
     6061 gaaatagtac acttgcttcc gcccgatcgc tctgtgcctg tagctcaatt ggatagagtg
     6121 ttggcctccg gagccaaagg ttgtgggttc aagtcccacc aggcacgcag cgatcgggcg
     6181 cgttcgcgcc gttccgcata aaatcaaaaa attgaggccc tcatgagaaa aaagaaaagc
     6241 gcacggccgc cggtcatcgg cgtcgatttc gacggcaccc tgtgccagat attcaaaagc
     6301 aacgtcccct acatccccaa gagcgtcatc cgggggaaac ccaacaagaa cgtcgtggaa
     6361 gcctgccgcc gctaccgtcg gtccggatgc cgcatcgtgg tgtatacctc ccgctggtgg
     6421 ggcgactacc actggctcaa acaatggctg gacaaatacg acgtgccgta tgacgacatc
     6481 gtgcccggca aattcaaggc cgacgccttc atcgacgaca ccaccgtcaa tccgagcctc
     6541 gaccgggact gggaagaccg cctgtgcacc atcctgggca aggacctccc ccgcggcaaa
     6601 tgtcccgaag gacaaaaatc ctgagatgat catcgccgtc atcggacaaa gcagttgtcc
     6661 cgattctttg gccaaaacgg ccgaatcggt cggagcggag atcgcccggc ggggacatgt
     6721 gctggtctgc ggcggcatgg gaggcgtgat ggaggcggcc tgccggggag ccaaacgcgc
     6781 cggaggcgtc accctcggca tccttcccgg caaggacaag aaggacgcca acccgtacgt
     6841 ggactatccc gtgctcaccg ccatgagcca cgcacgcaac gccgtcatcg tgcgcaccgc
     6901 cgacgcggtc atcgccgtgg gcggccgcta cggcaccctc tcggagatcg gactcgccct
     6961 ctccatcgac cgtccggtct acggtctgaa gacctggtcc atccggggcg tttccccggt
     7021 caaaacgccg aaagaggcgc tggaccgcat cgaagcccga ttcaaaaaat cctgaccctt
     7081 cccgccgccc cgccccgtca aaaatgaagg ccctcatcca acgcgtcagc gccgccggcg
     7141 tttccatcga aaaccagccg gaacgtccca tcggccgggg gctggtcgtc tttctgggga
     7201 tcaccgcgtc ggacaccgac aaagacatcc gctggctggc ggaaaagatc ctcaatctgc
     7261 gcgtcttccc caagcccccc gccgaggcgg cggccgcccc ctcggacagc gaattcgacc
     7321 tcgccgtcgc cgacatcggc ggcgaaatac tgctggtctc gcagttcacc ctctacgccg
     7381 acgtgcggcg gggccggcgg ccggatttct ctcgggccgc gccgccggca caggcgcaaa
     7441 tcctttacga aaagttcgtg acgcgtctgc gcggcctcca ccccaaactc gtcaccggcg
     7501 agttcggcgc ccggatgcgc gttcgcatcc aaaacgaggg gcccgtcacc ctgctcatcg
     7561 aatcaccggc cgacgccccg gcggtcgaaa cagcatgacg tcgcctcctc ccctccagaa
     7621 atttctcgac gcttttaaaa aacgcgcctg gctcctgaaa cccctctccc cgctggcgcg
     7681 gctggatctc cgcgtctggg cactggtgct gatcctgtgc tgcggcgtca gcggcgtcct
     7741 tttctggttc ctccgccggc cggccaacgt gctgttcatt gaaccgctcc gggcggaatt
     7801 ttatcacgac caatttttgg aatggaccct ccgggcccaa cccgccgccc tggactggaa
     7861 actccggaaa atgaagttca acacctggct ggaacgcagc ggcgaacgcg tcgtctccgt
     7921 cggcgacatc cgggagacgg tcctgcgata cgacaaaaag gccggacttt ggcgcgcgcg
     7981 catcccctgt ccctggaacg ccccggccgg ggactacgcc atccgggtgg acacctccgc
     8041 cctgccggtg gaatcgctcc ggctccgcgc caggccgctc cgggtcgcgg accgcgcttt
     8101 caagcccctg ccgccggggt tcggggtgct gacgctggaa agcatcggcc cgctgcgcaa
     8161 actcctcgcc ccggacggca cccgcaagga cggcaccgcc ctggcggaat gggctgaatt
     8221 catcggcgcc gacgcggtcg tcatccaggg cgccgagtcg ggagggtacg gcaaaaaact
     8281 tccggccgag ttcccctgga tcacccaatc caaggccgcc atcaaaacgg tggcgcggga
     8341 atgccacaag cgcggcctga aattcggcgt atacgtcatg agctacatgg tcggcggcac
     8401 gccggacaaa tcgcccgatt acgactacgg atggatctac gaggacggcc ggatgatcaa
     8461 cggcctggag atgcgcaagc ggcgcggcgt ctccatcacc gacccgcggc ggataaaaga
     8521 catcatcaac gtggcccgcc catggtatga catggaagag gtggacatcg tcggactgga
     8581 ctacatccgc cccgtgttcg gcggttacga actggtggac gccttcgtgc gggacatgcc
     8641 ggtcaccccg ccggcggact gggacaaacg ttccctccag caacgcatga cgtggctctt
     8701 cacccaccgt tccgccggcg gacataattt catcgagcaa tggtactggt accgcgccca
     8761 ccggaccgcc caggtcgtgc gcgccatcaa ggccggcctg ggcgacaaga aaccgctctg
     8821 ggccttcacc ctctcctggg aaaaaggctg gcaccacgga caggacccgg tgatgatgcg
     8881 cgacgcgggg atcgacatcg acgccatcat ggcctacgaa gccgacagcc ggcagttcca
     8941 ggggttcgtc aaacagtggc gggaatacgt tcgccgaaaa gaggtcaacc tcctggtggg
     9001 cgacgccgtg gactgggtgt tgcaccagag gaccctgaac cccgccgggc cggaggattt
     9061 ctacaaccgc gacatcctgg cggtgtcgca gttccatgcc gacggtccgg ttcgcggggt
     9121 gttcgtccac gactatgtgc gggccacccg cgggcgcctc ggcccctact ccacgttgga
     9181 gtggttcctc gccggaggcg ccatcatcac cacgacctgc cggctcaacg gcgccctcgg
     9241 ctacgacctc actctctccc ttccggaccg tgccgccgcg ggggaaaccg tgaccgggaa
     9301 attgacgctc gggcggaaac gccccgccgg aacgatccgc gcccatctct acagcgccgc
     9361 cgacgtgtcg atgccggatc gtgatttcga actctccccg tccacccccg ccgtcgcgtt
     9421 cacctttcgc tgggtgcccg accacacctc cgctcctcgc ggacaccgct cgttcgtggc
     9481 cgtccgcgcc gaaagaacgg ctccgtcggc ggaacggtgc caaattcata taatgtactt
     9541 tcagggccag cgcgggaata aaacgccgga aaaaccggcg cccaaaaacg agggggcgac
     9601 cgaaaaccgg gatgagacca aacccaaata aaaaccccgt cctcatgagc ttcgtcctcg
     9661 ccatgcttct ttccaacgcc gcgcggcttt tcgccggcgc tccgcctaaa accgatcccg
     9721 ccgccgatcg acggaatgag atggtccgct cccagattga acgccgcggc gtcaaagatg
     9781 cccgggtgct gaacgccttg cgccggatcc cgcggcacct cttcgtcccc gaagaaatgc
     9841 gttcctcttc ctacgatgac gaagcccttc ccatcggcgc cggccagacc atctcccagc
     9901 cctacgtcgt ggccgccatg accgaacgac tccggctcac cggcgaggaa cgcgtgctcg
     9961 agatcggcac gggctccgga tatcaggcgg cggtactggc ggaactcgcc gcccaggtct
    10021 attccgtgga aatcattccg gaactggccg accgggcccg gaagaccctc tcttctttga
    10081 actattccaa cgtccacgtg cgcaccggcg acggcgggaa gggctggccg gaaaaagcgc
    10141 cgttcgacgc cgtcatcgtc accgccgcgc cggaagatgt cccacccgct ctcctggagc
    10201 agttgaaaat ggggggccgg ctggtgatcc cggtgggtcc gcgggaaaat caggacctgg
    10261 tgtgtttcca taaaacgccg gacggggtcc ggaaagaaat cctgttcccc gtccgtttcg
    10321 tgccgttcgt gaggggagaa tgaagaaatc ccttttgatg ctccttctgt tgaccgccgg
    10381 cggcgtcacg gtccgggccg aaccgccgga ccccttcgcg tcctttgaaa tcccctcgtc
    10441 gaccaaaccg gcgagcgccg agatgtcgcc gttcgtgaaa gaactggcga ccacgttgaa
    10501 ttggccggcc gaccaactgc agacgctgga acgccgcggg ttcggccgca ccgagatggc
    10561 gacgttcgtc gccatcgcaa aaaaaacgtc gaaaccgtgg gccgaactca tcaaggcgcg
    10621 ggaaaagggc gccaccctcc ggcaaatgac ggaggaggcg ggcctggtct acaacgacgt
    10681 cttccgcgaa tcccgccgcg ccaaacagaa tgtggagaac cgcctgaaaa ataccgccgc
    10741 ctcgaaacaa tgagccccga ccgtcccatt cgatcccgcc gccggccctg acatatcccc
    10801 gacgacccgc tccgtcccag acgcaccgcc gaccgagacc cctttgtcgt ccgtgcacaa
    10861 tccgacgtcc gatttaattc aaggctcaac cgcttttcct ttattccctg ttcattattt
    10921 tctaagatgc ttttttagac tccggaggag ggacccgtgg ccataaaaaa tttcgtgctg
    10981 gacaccaacg tcgttctcta cgaccccaac gccatcttca gcttcgagga caacaacgtc
    11041 atcatcccca tctgggtcgt ggaggagatc gaccggttca agaaggacat caacgacacc
    11101 ggccgcaacg cccgggaact ttcgcgccac ctggacgccc tccggaaaaa aggcgacatc
    11161 acccaaggcg tgccgctcga gagcggcggg cagctgcagg tcatcacctc catcccgctt
    11221 tccccggatg cgccggagtt cctcaagaaa gaatcttacg acaacctcat cctcgccacc
    11281 gcgttctacc tcaaaaagaa aaatccgccg gtaatcttca tcaccaagga cgtcaacctg
    11341 cgcatcaaag ccgacgcgct gggcatagac accatggact acgagaccat gcgcatcgaa
    11401 ccggaaggac tgtacacggg gtggaaatcg ctgaccgtgc cggaatccct catctcggat
    11461 ttttatcagt ccaagaaact cgacacggcg aaattgccga agaacaactt ccacgagaac
    11521 gaattcgtct gcctgcaggg ggagacgaac gcgtcgcaga gcgcgctggg gatctaccgt
    11581 aaaggcgaag tccaactcct gccgccgctg ccgccggcgg tgtggggggt gaagccgagg
    11641 aacctggaac aacagttcgc cctgcacctc ctgctcgacg accgcattcc gctggtgacc
    11701 ctcaccggga aagccggcac ggggaaaaca ctgctcgctt tggccgccgg gatggcgaag
    11761 accctggacg agggcattta ccagcgggtg ctggtttccc ggcccatctt tccgatcggc
    11821 cgggacatcg gctacctccc cggcgacgtc aaagaaaaac tgcagccctg gatgcagccg
    11881 atcttcgaca acatggagtt cctcctgcac accgccaaaa acgacaagaa agggccgcgc
    11941 tacaacgccc tcatcgaaca gggactgctg cagatcgagg cgctcaccta catccgcgga
    12001 cgcagcatcc cgaaccagta tttcatcgtg gacgaggcac agaacatgac accccacgag
    12061 gcgaagaccg tcatcacccg cgtgggagag cacacgaaga tcattttcac cggggacatc
    12121 caccagatag acaatttcta caacgacgcc tcccacaacg gactttccta catcgtcgaa
    12181 cacctgaaag ccgaagggct ggcgggacac atgaccctga ccaaagggga gcggtccgcc
    12241 ttggccgagt tggcgtccaa cctcctgtaa gatgccgcct ctcgaatcgc aaccggacca
    12301 ggaccgtcgg aaaa
//
LOCUS       DAOTFD010000059        12142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3674_length_12142_cov_3.889054, whole genome shotgun sequence.
ACCESSION   DAOTFD010000059 DAOTFD010000000
VERSION     DAOTFD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3674_length_12142_cov_3.889054"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5079_05190"
     CDS             complement(<1..320)
                     /locus_tag="P5079_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29417.1"
                     /translation="MGGDARSCSVLLAGVDDLRVPAVASALAKFKKIPFQDAAVLARH
                     AWGLVGQDLSEPEALELKAGLEKAGVRARIVPSGALRPLIPARTISHLELNAEALRFA
                     AEDKT"
     gene            complement(310..2403)
                     /locus_tag="P5079_05195"
     CDS             complement(310..2403)
                     /locus_tag="P5079_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29418.1"
                     /translation="MPNKFILRWGAPTVVAFVTAVVFFPVLKNGFVNWDDAANFLQNL
                     SYRGLGWPQLRWMWTTFHMGHYIPLSWMTLGLDYLLWGMNPFGYHLTNLLLHAANATV
                     FYFLATRLLRLGFRGDSSEGAGDISWAAVFAALLFSVHPLRVESVAWITERRDVLSGF
                     FYLLSIYYHVRAFEENETGGVSPHRRWSLFFYGLALLSKSVAVTLPLTLVLLDIYPLR
                     RLGGADGWGWSPLARRVWQEKGPYVLLALPIAYVTVLAHRDIKAPLEYFGLLFRAGLV
                     FYQMVFYLAKTVWPSGLSPLYELPLNLESPPGGYFLCAGGVVAVSVSLLVFFRRLPAV
                     GVAWWHYVLALLPMTNFLLIAPYRAADRYTYLPCLSWALLAGAGLVWWSRRSKPGRGA
                     LLFWGAAGLVLFFGVRTWGQIPVWRDSNSLWTHVLRVEKKSAVAYDVLGRMFLDEGDP
                     VRAAGFFQKALELRPGFGFANANMARILQEAGRPHDASAYYQRALASLPNDFTLLNGY
                     ATCLMELNRSAEAKEFLGRAIAVRPTAAEPHYNLGMLLAGEGRDVEALAAWRTALDLD
                     PADVDVRVQIGLLLSRKGDLIGAVSHYRAALDLNPASLEAHYNLGMLLKQGADWRTAF
                     PHFRKALEINPRLPQVWLELGDLYRKSGEIDEAANAYRQALALNERFQAAAVALRSLQ
                     KKAGRASSRGAHDGR"
     gene            complement(2461..2961)
                     /locus_tag="P5079_05200"
     CDS             complement(2461..2961)
                     /locus_tag="P5079_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29419.1"
                     /translation="MPKEPELKEVLHILRKEPYVSAIGKLREMGVLHKHVIQTELGEW
                     VAYKLFGGQRMGSAHPVYDLVTPSGKTVKARTHYKEEMRTAPWSAVGQKHADIYIIFL
                     LKEDVGLEKALGGTRQELQASGALRLERKHGPRLHWTMMPELTPEKAKELGAADVTAL
                     QQWKSH"
     gene            complement(3011..4144)
                     /locus_tag="P5079_05205"
     CDS             complement(3011..4144)
                     /locus_tag="P5079_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY29420.1"
                     /translation="MSETQRFVFFLSVVLAVWTAMHLYVFSRLWTLPRLNSPAAHKTI
                     LLAGFFLWSAYFLGRFLAHRGYVSLAGFLEVAGAVWMGILFLALAFLFFADVATGFGW
                     LFASGVPAVRSAALLLAVFFSVFALVQGLRPPVVKDIEVPLAGLPAALEGKVLVQLTD
                     LHLGTLLRDRWLRRILRQVESLRPDMVAVTGDLVDGDVSQVEPLLPLLRELRAPLGVW
                     AVTGNHEIYAGRERSLRLLEAAGYRVLTDSAMEAAPGLVMGGVDDLSARRQFGVVGDP
                     LQKALGSRPNGAVIFLCHSPLLEARAAELGAGLMLCGHTHNGQIWPFGYIVRLFYPRI
                     AGRFTVGNMEMLVSRGAGTWGPPMRLFQRGEIVRVTLRSKAAS"
     gene            complement(4239..5477)
                     /locus_tag="P5079_05210"
     CDS             complement(4239..5477)
                     /locus_tag="P5079_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HRY29421.1"
                     /translation="MPKIYLIDVTNRDGVQTSRICLSKLQKTIINLYDSQMGIFQSEF
                     GFPVTHHETNYLNANIELAEKGVFGDLRLSGWIRAIEEDVEETFKHVPRIKYLNLSIS
                     TSDQMIMHKFKGKLDHDSIISEMTDAVKLARKKGALAVGVNAEDASRTGMDYLIRFAQ
                     AARDAGADRVRYCDTLGYDTPVTIYNRIKQLAEAVKCPIELHCHNDLGLVVANSVMGA
                     LAANDAGVDAYINTCVNGIGERAGNADLVAVILAIKHGSGIEGKYQLDEKINLKMAWK
                     ICKYASYAFGVPIPINQPGVGSNAFAHESGIHADGALKDRRNYELYEYEELGRGEPEV
                     IETGRQITVGEYSGIKGFRNVYDKMEIAFKDDAEASRILELARYANVHNQKPLVDEEM
                     KFIAQHSDIARKIMTMAPLS"
     gene            complement(5654..7240)
                     /locus_tag="P5079_05215"
     CDS             complement(5654..7240)
                     /locus_tag="P5079_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29422.1"
                     /translation="MFLAAEVVLRIFYPQPDLPVWSPESLEIIQDNQRAFFEKTFRDG
                     LWRLVQVPRKNAYTPPQEFLFDKPAGSARILVVGESSSVSLGRSLRQLLELAGLPDIA
                     EVMNCGSPAGTLEDVGRRLDESLRYSPDVVVLLFGHNVFYRAPSLNSLSFRIGAFAGG
                     SRIVRWILHAKMEQAINDPERWGEFEDFLRRSVRRARRRNVAIVLCTVPSNLWYPPGV
                     AGWELYDARFMEAGYLHLSGKRREAIALLEDLVARRSTALWHFQLADWQHEEGNDRRA
                     LDHLTEALNLDPEIFRATDRTNAIIRRVAIESGVGLLDIEARLKKAVPDGIPGWLDFE
                     DNQHLVGSGTRWAASSLTGLFVQQGRFPADTRRIWEAYSERYARDEIFPLERYLRGAA
                     FWAQRMENANARAIPYKVAAAYRENPREVLRVSRALLGRDWWAAEVLPRWRAILLLQV
                     SEGLWRAGERDLSFRLAARAAEWDDAWEEPLLQMGKCHLRLGNRDEARRCFERARRRN
                     HERVEVAFFLRRLRSEANGS"
     gene            complement(7342..8337)
                     /locus_tag="P5079_05220"
     CDS             complement(7342..8337)
                     /locus_tag="P5079_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29423.1"
                     /translation="MGDKRGVLMGDPTTFWRTAGDNPHARTRWGVRKFVRRSRAVEQW
                     HRLARTLLELGVEIGVLPAAGAPGLLFPSSAGFLAGADSATPFSQKRYHLSRLPNRTA
                     ENAVISAFLRAMGFSVQAFPQRFGGGSDFLSSEGCFLFSSGVIRRPRFVFSKGWPPFR
                     RCYGFQSAPSALDALAESARGKKVLPVTLIRETHFSGNTCLCPFGKNREFLMAYLPAL
                     SESSRARLKEQFGERLLGLSEPDGEGFAANAFQVDVDEPVLLLPHTVSEALQAQVRER
                     GVRPVLVDVSEFFQKAGGAVASLIGDLGPLPTDDAELSPGAVEFRRENSYPALFA"
     gene            complement(8488..8862)
                     /locus_tag="P5079_05225"
     CDS             complement(8488..8862)
                     /locus_tag="P5079_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29424.1"
                     /translation="MKKKKFSSQDTNRPAAASPAPAPASSSTTTPKAAKTPKGSIQID
                     YPLEGEILTSGCYTFRLGASPTDRVEVSIDGKDWLPCRECVGYWWFDWSGYGSGAHSL
                     EARIPSGKRYLKSKLRQFTVLI"
     gene            complement(9153..9992)
                     /locus_tag="P5079_05230"
     CDS             complement(9153..9992)
                     /locus_tag="P5079_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HRY29425.1"
                     /translation="MNTLSTEKRKMVVGALVEGNSIRATVRMTGVAKKTVMRILCEVG
                     KACWEYQQKSLVNLPCKRIECDEIWSFCYAKDRNLPEGKRDKWGYGSVWTWTAICADT
                     KIVPCWFIGQRDIHASNAFMRDLADRVSDQVQITTDGHNTYLQAVLGAFGRNANYGQI
                     VKRYRAMPGMTEGKYSPGECVSSYSMHVSGNPDADHISTSCVERQNLTMRMSMRRFTR
                     LTNGFSKKIQNLQWAVSLHFMNYNFCRIHSSLRVTPAMAAKVTNKVWEIEDVLALAYG
                     ISK"
     gene            complement(10262..10828)
                     /locus_tag="P5079_05235"
     CDS             complement(10262..10828)
                     /locus_tag="P5079_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29426.1"
                     /translation="MSHKIQIKLGQFEFSAEGDEATVKDQYARFMSFVDKTTQGNPAA
                     LVEKATPVKNENGDKAEPIDNKASGNVSNVDYEKIFSKDPTGVSLKIHPDGKAGDDLS
                     QARYSMLLILYGYKNVFGKEMVSAVGTSASMKKSGIGFRRLDVVADGLYSDNLINKTG
                     QRRGVQYNITNKGSNEAEKIIREMRIKY"
     gene            complement(11305..>12142)
                     /locus_tag="P5079_05240"
     CDS             complement(11305..>12142)
                     /locus_tag="P5079_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29427.1"
                     /translation="GVAGGAADIHRAYGEDLEPAKRDYPVLVARLSLAWGSVAKVAPG
                     LAGAVGSRVLKELLALRDRLSPDDAAGDQRKALETLERLGASLPTTPSALSLLPAVQL
                     ADAFGPAVEAPGFWEGIAERGGETVLDSAGRAWPTVSVLVSDKTSTELKRHLEKRQGE
                     LRAQGRPSAKVESLLAAWNAGRVFVFSRDELAARDALTADGLIDVAKLLGRFDLPVSD
                     DHVIRIITRDPALWWTKGLPASRQELVEFLLLLTKDLYFIATPAHIEEYLAAQKVLAI
                     QA"
BASE COUNT         2740 a         3806 c         3297 g         2299 t
ORIGIN      
        1 gtcttgtctt cggcggcgaa acgcagggcc tcggcgttca attccagatg ggagatcgtt
       61 cgggccggga tcaacggacg caacgcgccg gacggaacga tccgggcgcg caccccggcc
      121 ttctccaggc cggctttcag ctccagcgcc tccggctcgg aaagatcctg ccccaccagg
      181 ccccaggcat ggcgcgccag gacggcggcg tcctggaacg ggattttttt gaacttggcc
      241 agggccgacg ccaccgccgg cacccgaagg tcgtcgacac cggccaagag caccgaacaa
      301 ctccgcgcgt caccgcccat cgtgcgcccc tcgggacgat gcacgtcccg cctttttctg
      361 caaactcctc aaggcgaccg ccgccgcctg aaatcgttca ttcaacgcca acgcctgacg
      421 gtaggcgttc gccgcctcgt cgatctcccc cgacttccga tacagatcgc caagctcgag
      481 ccagacctgg ggcagtcgag gatttatttc cagagccttt cggaaatgag ggaacgctgt
      541 cctccaatcc gccccttgtt tcaagagcat ccccaaattg tagtgcgcct ccaaagacgc
      601 ggggtttaaa tccaaagccg ctcggtaatg ggaaacagcc ccgatcaaat cgccctttct
      661 cgacaacagc agaccgatct gaacgcggac gtcgacgtcg gcgggatcca aatcgagcgc
      721 cgtccgccac gccgccaaag cctcgacatc ccgaccttcg cccgccagaa gcatgcccag
      781 gttataatga ggctccgcgg cggtcggccg gaccgcgatg gcccgcccca aaaattcctt
      841 ggcctccgcc gagcggttca attccataag acaggtcgca tatccattga gaagcgtaaa
      901 atcgttcgga agggacgcca gcgcccgctg ataatacgcc gaggcatcgt gcggacggcc
      961 ggcctcttga aggatccggg ccatgttggc attggcgaaa ccgaaccccg gccttaattc
     1021 cagcgccttt tgaaaaaaac cggccgcacg gaccgggtcc ccttcgtcga gaaacatcct
     1081 ccccaacaca tcgtaggcca cggcggactt tttttccacc ctcagaacat gcgtccaaag
     1141 ggaattggaa tcgcgccaaa ccggaatttg tccccacgtg cgaaccccga aaaagagaac
     1201 gagcccggcc gcgccccaga aaagaagggc cccccgcccg ggttttgaac gacgactcca
     1261 ccaaaccagc cccgccccgg ccaacaatgc ccaggacagg caagggagat acgtgtatct
     1321 atcggcggca cggtaaggag cgatcaaaag aaaattcgtc atgggaagca acgcgaggac
     1381 gtaatgccac caagccaccc ccaccgcggg aagacgccga aaaaagacga ggagggacac
     1441 ggaaaccgcc acgacacccc ccgcacaaag gaaataaccg ccgggcggac tctccagatt
     1501 cagcggcaat tcatacaggg gcgataaccc cgacggccag actgtctttg caagataaaa
     1561 aaccatctga tagaaaacga gtcccgcccg gaagagaaga ccaaaatatt ccaacggggc
     1621 cttgatgtcc cggtgcgcca acacagtgac ataggcgatg ggcaacgcca acaggacata
     1681 cggtcccttt tcctgccaga cccggcgggc caaaggcgac cagccccaac catcggctcc
     1741 tcccaaacgt cttagaggat agatatccaa caaaaccaag gtcaagggga gggtgaccgc
     1801 gacggatttg gacaacaacg ccaacccata aaaaaacaac gaccatcgcc gatgcggaga
     1861 tacccccccc gtctcgtttt cctcgaacgc cctcacgtga taataaatcg acagcagata
     1921 gaagaaaccg gaaagaacgt cccttcgttc ggttatccag gccacggatt ccacgcggag
     1981 cggatggacg gaaaacagca gcgcggcgaa aacagccgcc caggaaatgt ccccggcccc
     2041 ctccgaagaa tcgcctctaa accccagacg cagaaggcgg gtcgccagaa aataaaagac
     2101 cgttgcgttt gcggcgtgaa gaagcagatt cgtcagatga tacccgaaag ggttcatgcc
     2161 ccaaaggaga taatccaaac ccagggtcat ccacgacagg ggtatgtaat ggcccatatg
     2221 gaacgtggtc cacatccaac ggagttgcgg ccagccgaga ccgcgatagg aaaggttttg
     2281 gaggaagttg gcggcgtcgt cccaattgac gaaaccgttt ttcaaaaccg ggaagaaaac
     2341 gaccgccgtc acaaacgcca ccacggtcgg cgcaccccac cgaagaatga acttattagg
     2401 cataccggga acgatcaaaa ggaccctccc ccgacgaacc ggagagtcac gaggaaaata
     2461 tcaatggctt ttccattgtt gcaacgcggt cacgtccgcc gcccccaact ccttcgcttt
     2521 ttcgggcgtc aactccggca tcatggtcca atgcaggcgc ggcccgtgtt tccgctccaa
     2581 gcgcaaggcg ccgctcgcct gaagttcctg ccgggtgccg ccgagggcct tttccagacc
     2641 gacgtcctct ttcaatagga aaataatgta gatgtccgcg tgtttctggc ccaccgccga
     2701 ccacggagcg gtgcgcatct cctctttgta atgcgtccgg gccttgacgg tctttcccga
     2761 cggcgtcacg agatcgtaga cgggatgcgc ggaacccatg cgttgcccgc cgaagagttt
     2821 ataggccacc cactccccca gttcggtctg aatgacgtgc ttgtgcagca cgcccatctc
     2881 ccgaagttta ccgatggccg agacgtacgg ttctttccgc aaaatatgca ggacttcctt
     2941 caactcaggc tctttcggca tattcctctc tccccgatgc gttattttcc ccacttgtcc
     3001 ctcgtcgaaa tcaggaagcg gccttgctcc tcaaggtcac gcgcacgatc tctccccttt
     3061 gaaaaagccg catgggcggc ccccacgtgc ccgctccgcg ggaaaccagc atctccatgt
     3121 ttccgacggt aaaacggccc gcgatccgag gataaaaaag acggacgatg taaccaaacg
     3181 gccaaatctg cccattgtgc gtatgtccgc aaagcatcag acccgccccc aactccgccg
     3241 cccgcgcctc caggagcggg ctatggcaca ggaagatcac ggccccgttc ggccgcgacc
     3301 ccagcgcctt ctggagaggg tcgccgacga ccccgaactg ccgtcgcgcc gaaaggtcgt
     3361 ccacccctcc catgaccagc cccggagccg cctccatcgc cgaatccgtc aaaacacggt
     3421 acccggcggc ttccagcagc cgaaggctcc gttcgcggcc ggcgtatatc tcatggttcc
     3481 ccgtcaccgc ccacaccccc aacggcgccc gaagctcccg cagcaacggc aacagcggct
     3541 ccacctgcga cacgtcgccg tcgacgaggt cccccgtcac cgccaccatg tccggccgca
     3601 gggattccac ctgacgaaga atccggcgca gccaacggtc ccggagcaag gtgcccaaat
     3661 gaaggtccgt caactgcacc aggaccttgc cttccagcgc cgccggaagg cccgccagcg
     3721 ggacctcgat gtctttgaca accgggggac gcagaccctg aaccagcgca aaaaccgaaa
     3781 agaaaacggc caggagaagg gccgccgaac gcaccgccgg gaccccggag gcaaacagcc
     3841 atccgaaacc cgtcgccaca tcggcgaaga agaggaaagc caacgccaaa aacaagatcc
     3901 ccatccaaac ggcgccggcg acttccagaa atcccgcgag ggaaacgtag ccccggtgcg
     3961 ccaagaaacg gcccaggaaa tacgccgacc aaagaaagaa gccggcgagc aagatggttt
     4021 tatgcgctgc cggagagttc aggcgcggga gggtccacag gcgggaaaaa acatagagat
     4081 gcatggcggt ccataccgcc agcacgacgg acagaaaaaa cacgaaccgc tgggtttcgc
     4141 tcaacgggac ctcatggaaa gagggcgatg gtcccccgga tgaaacgcgc accgcccgga
     4201 ggaggggcgg agagggacga attaccggaa aaagaacctc acgacaacgg cgccatcgtc
     4261 atgatcttcc gggcgatgtc cgaatgctgg gcgatgaact tcatttcctc atccaccagc
     4321 ggcttctggt tatgcacgtt ggcatagcgc gccaattcga ggatccgaga agcttcggcg
     4381 tcgtccttga aagcgatctc catcttatcg tagacgttgc gaaacccctt gatgcccgaa
     4441 tactcgccga cggtgatctg acggccggtc tcgatgacct ccggctcgcc gcgtcccaac
     4501 tcctcgtatt catagagctc gtagttgcga cggtccttga gcgccccgtc ggcatggatg
     4561 cccgactcgt gcgcaaaagc attggagccg acgcccggct gattgatggg gatggggacg
     4621 ccgaaggcgt aggaagcgta tttgcagatc ttccaggcca tcttgaggtt gatcttctcg
     4681 tcgagttggt atttcccctc gatgccgctg ccgtgcttga tggccaggat gacggcgacg
     4741 aggtcggcgt tgccggcgcg ctcgccgatg ccgttgacgc aggtgttgat ataggcgtcc
     4801 accccggcgt cgttggccgc cagcgccccc atgacggaat tggccaccac cagccccagg
     4861 tcgttgtgac agtgcagttc gatggggcac ttgaccgctt cggccagctg tttgatgcgg
     4921 ttgtagatgg tcaccggggt atcgtatccc agcgtgtcgc aatagcggac gcggtcggcc
     4981 cccgcgtccc gcgcggcctg ggcgaagcgg atcaaatagt ccatgcccgt gcgcgaggcg
     5041 tcctcggcgt tgactccgac ggcgagggcg ccctttttcc gggccaactt cacggcatcc
     5101 gtcatctcgg agatgatgga gtcgtggtcc agtttcccct tgaacttgtg catgatcatc
     5161 tggtccgagg tggagatgga caggttcaga tacttgatgc ggggcacgtg tttgaacgtc
     5221 tcctccacat cctcttcaat ggcgcggatc caaccggaca ggcggaggtc cccgaagacc
     5281 cctttttcgg ccaactcgat gttggcgttc agataattgg tttcgtgatg ggtgacggga
     5341 aacccgaact ccgactgaaa aatccccatc tgggaatcgt acaggttgat gatggttttt
     5401 tgcagtttgg acagacagat gcgggaggtt tgaaccccgt cgcgattggt gacatcgatc
     5461 aagtagattt tcggcatgac tttctccctc tttcggtcgt tccggcgaag atgctccgtg
     5521 ggacaaaaac agcccttccg ggccggaggc cgcctcctaa aaaagatatt ttagcaaacc
     5581 atgggaaacg ctgtcttcaa aaatcttggg cccggcgcgc gaaacgggaa gcggcggcga
     5641 cggagcgcgg agattaagac ccgttcgcct ccgaccgtaa cctgcgcaga aagaacgcca
     5701 cctccaccct ctcgtggttc cgccgacgcg cccgctcgaa acagcggcgc gcctcatcac
     5761 ggtttcccaa ccggaggtgg catttcccca tctgcagcag gggctcctcc cacgcatcgt
     5821 cccattcggc ggcgcgggcc gcgagccgga acgacaaatc cctctctccc gcgcgccaaa
     5881 gcccctcgct cacctgcaac agcaggatgg ccctccagcg gggcaaaacc tcggcggccc
     5941 accaatccct ccccaacagc gcccgagaca cccggaggac ttcccgcgga ttttcccggt
     6001 aggccgccgc cactttatag gggatcgcgc gcgcgttcgc gttttccatt cgttgcgccc
     6061 agaatgccgc gccccgcaga taccgctcca acggaaaaat ttcatcgcgg gcataccgct
     6121 ccgaataggc ctcccatatc cgccgggtat cggccgggaa acgtccttgc tgcacgaaca
     6181 accccgtcag ggaagacgcc gcccaacggg tccccgaccc gaccagatgc tggttgtcct
     6241 caaaatccag ccaccccgga atgccgtcgg gaacggcctt cttcaggcgg gcctcgatgt
     6301 ccaggagccc cacgccggat tcaatggcca cgcgccggat gatcgcgttc gtccggtccg
     6361 tggcgcggaa aatctccgga tcgagattca acgcctcggt caaatgatcc agggcccgac
     6421 ggtcgttccc ctcctcgtgc tgccaatccg ccagctgaaa gtgccagagc gccgtgctcc
     6481 gccgcgcgac cagatcctcc agaagggcga tggcttcccg acgtttcccc gagaggtgga
     6541 gatacccggc ctccatgaaa cgggcgtcgt acagctccca gcccgcaacg ccgggcggat
     6601 accaaaggtt cgacggaacc gtgcatagaa cgatcgcgac gtttcgccgg cgagcccggc
     6661 gcaccgaacg ccgcaggaag tcctcgaatt cgccccaacg ttcgggatcg ttgatcgctt
     6721 gttccatttt cgcatgcagt atccatcgaa cgatgcgact gccgcccgcg aacgcgccga
     6781 tacgaaaaga cagggaattc agggaggggg cccggtagaa aacgttgtgc ccgaaaagga
     6841 ggacgacgac gtccggtgag tatcggagcg actcgtccaa gcgccggccg acatcctcca
     6901 gggtgccggc ggggctgccg caattcatca cctcggcaat atcgggaagc ccggcgagct
     6961 caagcaactg ccggaggctc ctccccaggg acaccgagct ggactccccc acgaccagga
     7021 tccgcgccga ccccgccggc ttatcgaaga ggaactcctg cggaggcgta taggcatttt
     7081 tccgcggaac ctggacgagc cgccacaggc cgtcccgaaa ggtcttttca aaaaaggcgc
     7141 gctggttgtc ttgaatgatc tccagggatt ccggcgacca gacggggagg tccggttggg
     7201 gataaaaaat ccgcagcacg acctcggcgg ccagaaacac gatcgccaaa aaaaggaccg
     7261 tcccgatgga aaggacccat cgtctcctga aaaaccgtcc cgccggcgcc ggggacgggg
     7321 gaggtgtcct ctgatccgct gtcatgcaaa aagcgccgga tagctgtttt cacggcggaa
     7381 ttcgacggcc cccggggaca actccgcgtc gtccgtgggc aacggcccca gatcgccgat
     7441 gaggctcgcc accgcgccgc cggctttctg gaaaaattcc gaaacatcca cgaggacggg
     7501 ccgcacgccg cgctcccgga cctgcgcctg aagcgcctct gaaaccgtgt ggggaaggag
     7561 gaggaccggc tcgtcgacgt ccacctggaa agcgttggcc gcgaacccct caccgtccgg
     7621 ctcggacaac cccagcagac gctcgccgaa ctgttccttc aatcgcgccc gggaactctc
     7681 cgagagggcc ggcaggtagg ccatgaggaa ctcccggttc ttgccgaaag ggcaaagaca
     7741 ggtattgccg ctgaaatgcg tctcgcggat aagagtcacc ggaagcacct ttttcccccg
     7801 cgccgattcc gccagcgcat cgagggccga aggagcgctc tggaacccat aacagcgccg
     7861 gaacggcggc cagcccttcg aaaaaacgaa acgcggccgg cgaataacgc cggaagaaaa
     7921 aaggaaacag ccctcggacg acaagaaatc ggagccgccg ccgaaacgtt gagggaacgc
     7981 ctgcaccgaa aaacccatgg cccggaggaa cgcggagatg accgcatttt cagcggtccg
     8041 attcggaagg cggctcagat gataccgctt ctgcgaaaaa ggggtggccg aatccgcccc
     8101 ggccaaaaaa ccggcgctgg atggaaacaa cagaccgggg gcgccggcgg cggggaggac
     8161 gccgatctcc actcccaatt ccaagagggt ccgcgcgagc cgatgccact gttccacagc
     8221 acgggaacgt cggacgaatt tacggacccc ccagcgagtg cgcgcatgcg ggttgtcccc
     8281 cgccgtcctc cagaacgtcg tggggtcccc catcaaaacg ccgcgcttat ctcccatgtt
     8341 ttcccttgtc ccccatgctc atgaaaatat cgccatccga tttcctttcc ctcgaaacgc
     8401 tcccccgaaa cagaaccgcc gccggaggga gttcatccct cgggcggcgg attcctcaaa
     8461 aaactcccgc cggcggcaaa gccgccgtca aatcagaacg gtgaactgac gcagtttgga
     8521 cttaagatac cgtttgccgg acggaatccg ggcttccagg gaatgggccc cggacccgta
     8581 tccggaccag tcgaaccacc agtaaccgac gcattcacgg cacggcagcc agtccttgcc
     8641 gtcgatggaa acctccaccc gatcggtggg agaagcgccc agacgaaatg tataacaacc
     8701 gctggtgagg atttccccct cgagcggata atcgatctgg atggaaccct tcggtgtctt
     8761 ggcggctttg ggagtggtcg tggaggaact ggccggtgcc ggagccggcg atgcggcggc
     8821 cgggcggttc gtatcttgag aactgaattt tttctttttc ataacaaacg cctccaaaaa
     8881 ggttttgcct attttagcac ttttcggaga tttcggcaca gcttcgccgc ggccgccata
     8941 aaaacaaaag cgcccgctcc attaaagcgg gcgcctctct cccccgagag atctttaaaa
     9001 acaagggcca taacacaagc gactccctcc ggttcgctca ggtacggccc ttaaatcggt
     9061 gtttcgactt ccattaaaag aatacccgtt ctctgtaaac tgccttttaa cagattgtaa
     9121 cgaagttgta aaaataaggg tgtggtagtg ggctactttg aaatcccata ggccaatgcc
     9181 aaaacatctt cgatctccca gaccttgttc gtcacctttg ccgccattgc aggggtgacg
     9241 cgcaaggacg agtgaatcct acagaaatta tagttcataa agtgcaagga tacggcccat
     9301 tgaaggttct gaatcttttt agagaagccg tttgttagcc gggtgaagcg gcgcatgctc
     9361 atacgcatgg tgagattctg ccgctccacg caagacgtcg aaatatggtc tgcgtccggg
     9421 ttcccgctta cgtgcatgct gtaggaactg acacattccc caggactata tttcccctca
     9481 gtcattccag gcattgcccg atatcgcttc acaatctgcc cgtagttcgc attgcgaccg
     9541 aatgccccaa gaacggcttg caggtaggta ttgtggccgt ccgtggtgat ttggacctga
     9601 tcggaaacgc ggtccgcaag gtcacgcata aaggcattgg aggcgtgaat atccctttga
     9661 ccaataaacc agcaagggac tatcttggta tccgcacaaa tcgccgtcca cgtccaaacg
     9721 cttccatatc cccatttgtc ccgcttgcct tcgggaaggt tcctgtcttt ggcgtaacag
     9781 aaagaccata tctcgtcgca ctcgatacgc ttgcagggaa ggtttacgag ggacttctgt
     9841 tgatattccc aacaggcctt tcccacttcg cagagaatcc gcataacggt cttcttggca
     9901 acacctgtca tgcggacggt agcccgtata gagttaccct ccaccaaagc ccctacaacc
     9961 attttccgct tttccgtcga aagtgtattc attgtgctca cctcctccct ataagtatac
    10021 ttgaccgctc aagcattgtc aagagatatt tttatgctta accgctaaag gacttgacat
    10081 tatgatgttt gtgggctata ctagatatac agtttaagaa tcaataaagt ttagcaggtg
    10141 gggagtgcct tccgtcccgg tcaaaggttg ggttaggcac tccccttcgt tttaagcggg
    10201 cctcccacta acttaggccc ttcttatctt ctcataagat accccccatc aaagaataaa
    10261 atcagtattt aatgcgcatt tccctgataa ttttttcagc ctcgtttgat cctttattcg
    10321 ttatattgta ttgaactccc cgtcgttgtc cggttttgtt gattagatta tctgaataca
    10381 gcccgtccgc aacaacatcc aaacgcctaa atccaattcc agactttttc attgaggccg
    10441 aagtccccac agccgaaacc atctcttttc caaaaacatt tttatatcca taaagaataa
    10501 gaagcatgga atagcgtgct tggcttaaat catcacctgc tttcccatct gggtgaattt
    10561 ttagtgaaac accagtcggg tcttttgaaa atattttttc gtaatctacg tttgaaacgt
    10621 tcccgcttgc tttattgtcg ataggctccg ctttgtcccc attttcattt tttacgggag
    10681 ttgctttctc caccagcgcc gcaggatttc cttgtgttgt cttatcaaca aaactcataa
    10741 atcgagcgta ttggtctttt acggtagcct cgtcaccttc cgccgaaaac tcaaactgcc
    10801 ctagttttat ttgtatttta tgagacatat gccaccccca tgtgctatat ataggactac
    10861 aatacaattg tagtcagtat actggcatat gtcaaggctt tattttattt tttgttgctg
    10921 gaaatgacta tttgcttatt ttctgttttt ccaacgtgat tctgctgcct tcctggctat
    10981 ttctttccgt cggcgggatg acaatttctc tgctcgcgcc cggccccccc ggagaccccc
    11041 gagacggccc aaagcaacag cagcagcgtt tttcccttct ggtgttatag gcatttctat
    11101 tgtggcagga tcagtagtgg cttgttttac aatagaagcc gctagctgat taatgtcggt
    11161 gggtttctta atgcttctgc gctcatgcat acttaaatta taggcgatgc acaaaaaatg
    11221 tcaagggcta tttatcggaa gtgcgttata ttcgttttca aagtagccca ctaccaaggg
    11281 tgtggccgat gggccgagcc gagcttaggc ctggatggcc aacacttttt gagcggcgag
    11341 atattcctcg atatgggccg gcgtggcgat gaaatacaaa tctttcgtca acagaaggag
    11401 gaactccacc agttcctggc gggaagcggg cagacccttc gtccaccaaa gagcgggatc
    11461 gcgggtaatg atccggatga catgatcgtc cgaaacgggc aggtcgaacc ggcccaggag
    11521 tttcgccacg tcgatgaggc cgtcggccgt gagggcgtcc cgagcggcca gttcgtcccg
    11581 ggagaaaacg aagacacgcc ccgcgttcca ggccgccagc agactctcca ccttggcgga
    11641 gggacggcct tgagcccgga gttccccctg ccgtttctca agatgccgtt tcaattccgt
    11701 cgaggttttg tcgctgacga ggacggagac ggtgggccag gcgcggccgg ccgaatccag
    11761 aacggtttcc ccgccccgct ccgcgatccc ctcccaaaaa cccggcgctt ccacggccgg
    11821 accgaacgcg tcggccagtt gcaccgccgg cagaagagag agggccgacg gtgtcgtcgg
    11881 caacgacgcc cccaaccgtt ccagcgtctc caaggccttg cgctggtccc ccgcggcgtc
    11941 atcgggcgac agccggtcgc ggagagccag aagttccttc aaaacccgcg aaccgacggc
    12001 gccggccagt ccgggggcga cttttgccac cgagccccac gcgagagaga ggcgggcgac
    12061 gagaaccggg tagtctcgtt ttgcgggctc caaatcttca ccgtaagcgc gatggatatc
    12121 cgccgccccc ccggccacgc cc
//
LOCUS       DAOTFD010000060        11961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3740_length_11961_cov_4.817487, whole genome shotgun sequence.
ACCESSION   DAOTFD010000060 DAOTFD010000000
VERSION     DAOTFD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_11961_cov_4.817487"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..2240)
                     /locus_tag="P5079_05245"
     CDS             complement(390..2240)
                     /locus_tag="P5079_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRY29428.1"
                     /translation="MADEKFDAIVVGAGPAGVSAAVTLARAGLNTVVLERGEYPGAKN
                     VQGAVLYTRNLDDIVPGFWKDPQAALERPITEEKYWVMSDDSAAQFGFKSDRWRREPH
                     NCYTIIRVNFDRWYSKKAEEAGAQLFCGVTVSDAIKKDGKVVGIKTSEGDELYADAVI
                     ACDGVNSMLAQQVGLMDEWRSDEVALGVKEVLALPREKIEDRFCLEKDEGSTIEMFGS
                     ITRGMLGYGFLYTNKESLALGVGCKLSHFQKTCIKPYELLEQLKAHPILRRLLAGSKT
                     LEYSAHLIPEGGYHSLPPLYADGFLVAGDAAQMVNAAHREGSNLAMTAGKLAAETVIE
                     AKKAGDFSAKTLSAYQKKLEKSVVLPDLYLHRDLETLAEKTGHPAHLVAVCQPGPLLM
                     TTLILHPHLGKTFGSAGAPMNTDAERGFLSDFARTMGEPYIDLLTTALGSRVPKGLPG
                     EGRAIYDGRLQVLGFYILGMPQHFDNLKKLLTDLRDGNEETASRQMAFYQWYNYSHDF
                     PASFLRDTYKKVFVRNELIRGNLTVGERKIGVKDYPASVPIWALGGTQDNIAPCGQAI
                     GHVELIEGVAPADRLTLCCDAGHMGLFRSRKVLDLHYSRIIRFLLERSDR"
     gene            complement(2265..2636)
                     /locus_tag="P5079_05250"
     CDS             complement(2265..2636)
                     /locus_tag="P5079_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29429.1"
                     /translation="MAKILVADDEADVRELVKYALERDQHQVLETSSGSETLELLKKE
                     TVDLLILDVMLPGMDGYTLQLQLAQDKKTRFIPVIVLTALHPSKSMFAKFSQVREFVP
                     KPFDPMELAGLVQRTLASPVK"
     gene            complement(2697..3302)
                     /locus_tag="P5079_05255"
     CDS             complement(2697..3302)
                     /locus_tag="P5079_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRY29430.1"
                     /translation="MTSDVLVLNRQFYAIHVASWRRALTLLYLDHANVVDEEYRTYSF
                     ADWREMSRTLRQHPAGFVTTPSFRIAVPEVIALKIYDRLPVSEVKFTRRNIYEHYGYR
                     CCYCGKELPTDMLNLDHVVPRSRGGATDWSNIVTSCIPCNLKKADRLPKEAGLKLLLP
                     PTRPKWKGSASLVFRANFRVRQSWQKFIDNVYWNTELEEGR"
     gene            complement(3406..3633)
                     /locus_tag="P5079_05260"
     CDS             complement(3406..3633)
                     /locus_tag="P5079_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRY29431.1"
                     /translation="MAMKRLCRLFFPKNLIREPLVYRMAKECGVTFDIRETKIMADGG
                     TLLLALDGAEDSLGRAAEWLKAKGVRVEAAE"
     gene            complement(3638..3910)
                     /locus_tag="P5079_05265"
     CDS             complement(3638..3910)
                     /locus_tag="P5079_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRY29432.1"
                     /translation="MALVRIPSPLRHLTRKQSVVSASGGTVEEVFAALEKSCPGVREK
                     IIDETGQVRRFINVTLNGEDIKLKDGVKTPVSENDELRVVPAIAGG"
     gene            complement(3972..5435)
                     /gene="gatB"
                     /locus_tag="P5079_05270"
     CDS             complement(3972..5435)
                     /gene="gatB"
                     /locus_tag="P5079_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRY29433.1"
                     /translation="MASFEPVIGLEVHVQLKTKSKVFCGCPTDFGAEPNSQICPVCCG
                     YPGVLPVLNRSAVEGLVRTALALDCRVNSRSVFARKQYFYPDLPKNYQISQYELPLAE
                     HGHLDISTEAGPRRVRIHRIHLEEDAGKLLHAIGSRALDYSLVDLNRTGIPLMEIVSE
                     PDMHTAEEAAAYMEGLRAVLRYLGVSDCDMEKGFMRCDANVSLRPAGTTGLGTKAEVK
                     NMNSFRGVRDALAYEIERQTDLLEAGQKIVQETRLWDAQKGSTHSMRSKEEAHDYRYF
                     PEPDLVPIELSESFLDELRRSLPELPSVRRTRLEKERGLSGYDAGVITAEKPLADYFE
                     QALAAFEEPLRREAAKPLANWLTTELLGRLHAAQKGIEESPISSRRLAALVRLVLKGT
                     VSGKTAKTVFDDMFAGGGDPETIVEQKGLTQIEDEVLIGRWADEAIAGQPKSAQDVKA
                     GKAAALGALVGAVLKKSGGRANPQTVQRLLKQKLGAS"
     gene            complement(5451..5795)
                     /locus_tag="P5079_05275"
     CDS             complement(5451..5795)
                     /locus_tag="P5079_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29434.1"
                     /translation="MKYRRVFPSTVLLLFSACAAPAPKRAPGEAVSVRPVSMTTLSGR
                     VFRRVKFGGYLEFQSKSGDLYVLEGKTAKRIRPFLFGGDEGLPVTVTGFPLENVSHRA
                     GPVFEVVDYQWQ"
     gene            complement(5792..6250)
                     /locus_tag="P5079_05280"
     CDS             complement(5792..6250)
                     /locus_tag="P5079_05280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY29435.1"
                     /translation="MKKTPAGRRKKVPGEAVEVSAGGLVLGDGKLLMVKVRNLEGRVV
                     WTFPKGHLEKGETAEDAALREVREETGRRCRLRRPFGKVHYEFYRNGKHVRKTVHWFL
                     MEPLETLGEPDPEEVLDCRWFSWPESAEVAVYPSDKKILARLKKSSEVKK"
     gene            complement(6247..6867)
                     /locus_tag="P5079_05285"
     CDS             complement(6247..6867)
                     /locus_tag="P5079_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRY29436.1"
                     /translation="MDETKLKTLKRYFLTGLFVLIPVAFTVVTVFWLIGTLDGLLGGM
                     VRGLMGFHIPGLGIVMTVGLILLVGMLMSNLLFKQMFDLFEEALLRVPGVRVIYKTVK
                     ALTETFSPENQKSFRNVVLVEYPHPGSFSLGFVTGQTVLTVDGASQKHAVVYVPTNHF
                     YLGNTLVVPESKVLATTLSVQDGIQLVLSSGAGFPDQLLRRGLEQK"
     gene            complement(6860..8329)
                     /gene="gatA"
                     /locus_tag="P5079_05290"
     CDS             complement(6860..8329)
                     /gene="gatA"
                     /locus_tag="P5079_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRY29437.1"
                     /translation="MNLHELSARQIAEQVGARKVSAEEVTNAFLARIAAWDPRVKAFL
                     SVLKDTAVAQARAVDEQVRRGEKAGPLAGVPVAVKDNMCVRGTRTTCSSKILENFTAN
                     YDATVIAKLRAAGAVFIGKTNLDEFAMGSSTENSAFFTTRNPWNTDYIPGGSSGGSAA
                     AVAARLAPLALGSDTGGSIRQPATCCGVVGMKPTYGLVSRFGLIAFASSLDQIGPFAW
                     NIADAAALLGVIAGHDPHDSTSVNRSVPDYLPALTAGVKGLTAGLPEEYFRGGLDPEV
                     ERAVEESIRVLEKAGMRFEKISLPHTDAAVSVYYILAPSEASANLARFDGVRYGTRAP
                     EATTLLEQYELSRARGFGPEVKRRIMLGTYALSSGYYDAYYLKAQKVRTLIKRDFDDA
                     FQKVDLILTPTAPTPAFRIGERTDDPLRMYLSDIFTISCNLAGLPGLSMPCGFSSKGL
                     PLGLQILGKPFGEEQVFSAAAAYEQATDWNRIPPEPSRG"
     gene            complement(8326..8616)
                     /gene="gatC"
                     /locus_tag="P5079_05295"
     CDS             complement(8326..8616)
                     /gene="gatC"
                     /locus_tag="P5079_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRY29438.1"
                     /translation="MALTEKDVKHVARLARLSLSDEERERLRGQLERILDYMKNLEKH
                     ATGDVPPTSHVAPLSNVWRPDEVRRWEDVESILANAPERENSYFKVKKVIEP"
     gene            complement(8591..9985)
                     /locus_tag="P5079_05300"
     CDS             complement(8591..9985)
                     /locus_tag="P5079_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY29439.1"
                     /translation="MRETLKSATLFLGLVGFAAWPLWAQNRKAPDPLTDETVAVLKGY
                     VRIDTANPPGRELRAAEYLRGLLEREDIPSEIFVSSPGRANLLARLKAEKPTQPPLLL
                     IHHMDVVPADPAEWSIDPFAAKERDGYLWGRGALDSKITGALHLMTLFQLKREDVRLN
                     RDVLLLAVADEEAGGRWGSQWMVENHWDRMKPGFVIDEGGFGLIDVFSYDPVPVYACA
                     VDEKKVLWLKLTANGRSGHGSVPNTDNPIDVLRKALARMEQTFIRFGGRTPEPVRDLE
                     RAVPHPRKSPLLEAVRHNSLSVTSFQSWSGAAATPTVNVIPDRAVATLDCRLLPGEDE
                     QDLLAKLRRAVDDDRVRIEVTVQTRVTSDYRDYRSPLYDGLVRAVRRHDEKAVVAPFL
                     MPGATDSRFFRAKGVRCYGLVPLVLTEEEYLLMHASDERLPLDRLEKARRIMYDWVKN
                     FCLETDDGVDGKRR"
     gene            complement(9988..>11961)
                     /locus_tag="P5079_05305"
     CDS             complement(9988..>11961)
                     /locus_tag="P5079_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY29440.1"
                     /translation="KERLERMLGHGYPGMWAGTFHAFGAWFLRHEAGRLGYPRSFIIY
                     DSEDQRSLVNKCIRDLKLKTSKGTDSTVAWLIAMAKDTLQDYTRITCKLDFDPGPVIA
                     LYEERKKAYGAFDFGDLLKVPGEMLQSMPEVKGRYQSLFRYLLVDEYQDTNMAQYVML
                     MGLVNSEQNICVVGDDDQSIYSWRGANVRNILEFERDYPGAAVVKLEQNYRSTETILA
                     AAWKIIRRNKFRKEKRLWTDQAGGLPVRFQEFANELEEARAVARETARLLAAGEVVRR
                     DVAVFYRTNAQSRVLEDAFRRENIPYSVVGGVRFYERAEVRDVLAFLRVLLNPADSVS
                     LKRILNVPPRGIGKTTVQALEKYALAHGVSFYESIVQSARVPDLTSAARGNLQKFLDI
                     LRPLAVKMAVTGAAAMVQAVLEETGCWDHWQEQTDDDPEAAYRLDNLQELVNAAKEFE
                     ESSEDRSTALFLERVSLVSDIDGWNDADGSATFMTVHLAKGLEFPAVFMTGMEEGLFP
                     IGESAFDEEELEEERRLCYVGMTRARRHLFLTAAASRKIYGKSHWNVPSRFVQEAELA
                     SVPAPAPGRSEEYGAVVGAFDPDELNRAALPTPGGLRPLQVGMRVRHPSFGEGRIAGR
                     SGAGENLKVTVDFDSGMRKTIVARYASFEILKG"
BASE COUNT         2137 a         3721 c         3835 g         2268 t
ORIGIN      
        1 gtttgggttg ttgtgagggg aatataaccc acccgaggcg gcttgtcaag ggaaaatgtt
       61 gcactgcaac aaaatttttg tgcgaattgc ggattcgtta aataatatat taattcttat
      121 atataaagat aattctagtt tcgttttccg ccattttatt cttgctttat gtcgcagcgt
      181 gacatcccct ggagccctca cggggggttt ccggggacgg gaaattcccg gactacttat
      241 cctgcaatgg ttcttggtga gcggaggcgc aagcggcagt cggaacacct gagcttcagc
      301 ggaagtgcag cgcaacatcc ggcatttcag aaccaccaat aaaactttac atgaaaccgg
      361 tgcatcctgg caacgattaa aaacgttcct taccggtcgc tgcgctcgag aagaaaacgg
      421 atgatccggc tgtagtgcag gtcgagcacc ttgcgggaac ggaacaatcc catgtgaccg
      481 gcatcgcagc aaagggtgag cctgtctgcg ggggcgaccc cctcgatgag ttccacgtgg
      541 ccgatggcct gcccgcaggg ggcgatgttg tcctgcgtgc cgcccagcgc ccatatgggc
      601 accgacgcag ggtagtcctt cacgccgatc ttccgttccc cgacggtcag gttgccgcgg
      661 atcagctcat tgcggacgaa gaccttcttg taggtgtcgc gaagaaagct cgccgggaag
      721 tcatggctgt agttgtacca ctggtaaaag gccatctggc ggctggcggt ttcctcgttg
      781 ccgtcccgga ggtccgtgag gagttttttc aggttgtcga aatgctgggg catacccagg
      841 atgtaaaacc ccaggacctg cagccggccg tcatagattg cgcggccttc gccgggcaga
      901 cctttgggga cccggcttcc cagggccgtg gtcagcaggt cgatgtaggg ctcgcccatg
      961 gtcctcgcga aatcggagag gaacccccgt tcggcgtcgg tgttcatggg ggcgcccgcc
     1021 gagccgaagg ttttccccag gtgcgggtgg aggatgaggg tcgtcatcag cagcggcccc
     1081 ggctggcaga cggccacgag gtgcgcgggg tgtcccgtct tctccgccag ggtctccagg
     1141 tcgcgatgga gatacaggtc gggcaggacc acggattttt ccagcttttt ctgataagcg
     1201 gagagcgtct tggcggagaa gtcgcccgcc tttttggcct cgatgacggt ctcggcggcc
     1261 agcttgccgg cggtcatggc caggttggac ccttcgcggt gggcggcgtt caccatttgg
     1321 gcggcgtcgc cggccacgag gaacccgtcg gcgtagagcg gcggcaggga atgatatccc
     1381 ccttccggga tgaggtgcgc cgaatattcc agcgttttgg acccggcgag caggcggcgg
     1441 aggatggggt gggccttcaa ctgttccagc agttcgtagg gtttgatgca cgtcttctgg
     1501 aaatgcgaca gcttgcatcc cacgccgaga gccagggact ccttgttggt ataaaggaaa
     1561 ccgtagccca gcatgccgcg cgtgatggaa ccgaacatct cgatggtgct gccctcgtcc
     1621 ttttcgaggc agaagcggtc ctctatcttt tcccggggca gggccaggac ctctttgacg
     1681 cccaacgcca cctcgtcgga gcgccattca tccatgaggc cgacctgctg ggccagcatg
     1741 gagttcacgc cgtcgcaagc gatgaccgcg tcggcataga gctcgtcgcc ctcgctggtc
     1801 ttgatgccca cgaccttgcc gtccttcttg atggcgtcgg acacggtgac gccgcagaaa
     1861 agctgggccc cggcctcctc ggcttttttg gagtaccagc ggtcgaagtt gacgcggatg
     1921 atggtgtagc agttgtgcgg ctcgcggcgc caccggtcgg acttgaaacc gaactgggcg
     1981 gcggagtcgt cggacatgac ccaatatttc tcctcggtga tgggccgttc cagcgcggcc
     2041 tgcgggtcct tccagaaccc gggcacgatg tcgtccaggt tgcgggtata gagcaccgcg
     2101 ccctgaacgt tcttggcgcc gggatactcg ccccgttcca gcaccaccgt gttcagtccg
     2161 gcgcgcgcca gggtgacggc ggcggaaacg ccggccgggc cggcgcccac cacgatcgcg
     2221 tcaaatttct cgtcggccat gttgtctctc cttctgtcgg aaaattattt tacgggggag
     2281 gcgagggtgc gctgcaccag cccggccagt tccatcggat cgaacggctt ggggacgaac
     2341 tcgcgcacct gcgaaaactt ggcgaacatg cttttactgg ggtggagggc ggtgagcacg
     2401 atcaccggga tgaagcgcgt cttcttgtcc tgcgccagct gcagctggag agtgtatcca
     2461 tccatgccgg ggagcatgac gtcgaggatc aacaggtcca ccgtctcctt cttcaaaagt
     2521 tccagcgtct cggagccgct gctggtctcg agcacctggt gttgatcgcg ttccagggcg
     2581 tatttcacca gttcgcgcac gtccgcttcg tcatccgcta ccaatatttt ggccatctct
     2641 ccgcctcgtt caaacgatat tgtcccgcgt agtgtatcaa atccgccgcc ggccgcctat
     2701 ctcccctctt ccagctccgt gttccaatac acgttgtcga tgaacttctg ccacgactga
     2761 cgcacgcgga aattggcgcg gaaaacaagg gaggcgctgc ctttccattt cggccgcgtg
     2821 ggagggagca agagtttcag ccccgcctct ttcggcaggc ggtcggcttt tttcaggttg
     2881 caggggatgc aggaggtgac gatgttcgac caatccgtgg ccccgccgcg gctgcgcggc
     2941 accacgtggt ccaggttcag catgtccgta ggcagctcct tgccgcaata acagcaacgg
     3001 tagccgtaat gttcgtagat gttccgccgg gtgaatttca cctcgctcac cggcagccgg
     3061 tcgtaaatct tcagcgcgat cacctccggc acggcgatgc ggaaagacgg cgtggtgacg
     3121 aaccccgccg ggtgctgccg cagcgtgcgg gacatctcgc gccaatccgc gaaggaatac
     3181 gtgcggtatt cctcgtccac cacgttggcg tggtcgaggt acagcagggt cagcgcccgc
     3241 cgccacgagg cgacgtggat ggcgtagaac tgccggttca agacaaggac gtccgaggtc
     3301 ataaaattca ggctctctcc cctctccttc agtggagctc tggaagctga aaaacccatc
     3361 ccccaaaaat cgtcaggaac aaccgccgcc gaaacgactc aaaacctatt ctgccgcctc
     3421 cacccggaca ccctttgcct tcagccattc ggccgcgcgc cccagggaat cttcggcccc
     3481 gtccagcgcc aaaagcaggg tcccgccgtc ggccatgatc tttgtttcac ggatgtcgaa
     3541 cgtcacgccg cattccttgg ccatgcgata gaccagcggc tcacggatga ggtttttcgg
     3601 gaaaaaaaga cggcacagcc gtttcatcgc catcccctca tccgccggcg atggcgggca
     3661 ccacgcgaag ctcgtcgttt tctgaaaccg gggtttttac cccgtctttc agcttgatgt
     3721 cctcgccgtt gagagtgacg ttgatgaacc ggcgcacctg tccggtctcg tcgatgattt
     3781 tttcccgcac gccggggcag gatttttcca gcgccgcgaa aacctcttcc accgttccgc
     3841 cggaagcgga caccacggac tgtttccggg tgagatggcg cagcggcgac gggatgcgga
     3901 cgagggccaa aacggtctct cctttttccg gaaattctac cggagctccg gggacgggtt
     3961 caagggggct ctcacgaggc gccgagtttc tgtttgagga gccgctgaac ggtctgcggg
     4021 ttggcgcggc cgccggattt tttcagaacc gcccccacca gcgcgcccag ggccgcggcc
     4081 ttgccggcct tcacgtcctg cgcggacttg ggctggccgg cgatggcctc gtcggcccac
     4141 cgaccgatga ggacttcgtc ctcgatctgt gtgagacctt tttgctcgac gatggtttcc
     4201 gggtccccgc cgccggcgaa catgtcgtcg aagacggtct tcgccgtttt tccggaaacg
     4261 gtgcctttca aaaccagccg caccagggcc gccaggcgcc gggacgaaat gggggattcc
     4321 tcgatgcctt tttgggcggc atggaggcgg cccaacagct cggtggtgag ccaattggcg
     4381 agcggcttcg ccgcttcccg acggagaggt tcctcgaacg cggcgagggc ctgttcgaaa
     4441 tagtcggcga gcggtttctc cgcggtgatc acgccggcgt cgtatccgga gaggccccgt
     4501 tccttctcca agcgggtccg gcgcaccgac ggcagttcgg ggaggcttcg tctcagttcg
     4561 tcaagaaaag actcggagag ttctatcggg acgaggtccg gttccgggaa atagcggtag
     4621 tcgtgcgcct cttccttgga gcgcatggaa tgggtggacc ccttttgcgc gtcccacagg
     4681 cgggtctcct gcacgatctt ctggccggct tccagcaggt ccgtctgccg ttcgatctcg
     4741 taggcgagcg cgtcgcgcac gccgcggaaa gagttcatgt tcttaacctc cgccttggtg
     4801 ccgagaccgg tggtgccggc gggccggagg gacacgttgg cgtcgcaccg catgaacccc
     4861 ttttccatgt cgcaatcgga aacgccgagg taacgcagca cggcgcgcag gccttccata
     4921 taggcggcgg cctcctcggc ggtgtgcatg tccggctcgg agacgatctc catcagcggg
     4981 atgccggtgc ggttgaggtc caccaaggaa taatccaggg cccggctgcc gatggcgtgg
     5041 agaagtttgc cggcatcctc ttccagatgg atccggtgga tgcggacgcg ccgcgggccg
     5101 gcctccgtcg aaatgtcgag atggccgtgt tccgccagcg ggagttcgta ctgggagatc
     5161 tgatagttct tcggcaggtc gggatagaaa tattgttttc gcgcgaacac ggaacggctg
     5221 ttcacgcggc agtcgagcgc cagggcggtg cgcaccagcc cctccacggc cgaacggttg
     5281 agcacgggca acacgccggg atagccgcag cacacggggc agatctggct gttgggctcg
     5341 gcgccgaaat ccgtcggaca accgcagaag accttggact tcgtcttcag ctggacgtgc
     5401 acttccagcc cgatgaccgg ctcaaaagac gccacgggaa cctccccggt tcattgccat
     5461 tgatagtcca ccacttcgaa caccggcccc gcccgatggg aaacgttttc cagagggaac
     5521 cccgtcacgg tgacgggcaa cccctcgtcc ccgccgaaga ggaacggccg gatccgtttg
     5581 gccgtcttgc cctccaacac atagaggtcg ccggatttcg attggaactc cagatagccg
     5641 ccgaacttga cgcggcggaa gacccggccg gagagggtgg tcatgctcac ggggcggacg
     5701 gacaccgcct cgcccggcgc ccgtttcggc gccggcgcgg cgcaggcgga aaagagaagg
     5761 aggacggtgg agggaaaaac gcgccggtat ttcatttctt tacctcgctc gatttcttca
     5821 aacgcgccag gatcttcttg tccgacggat acaccgccac ctccgccgat tcgggccacg
     5881 agaaccaacg acagtccaat acctcttccg gatccggctc ccccagcgtt tcgagaggtt
     5941 ccatgaggaa ccaatggacg gtcttacgga cgtgtttccc gttccgataa aattcataat
     6001 gcacttttcc gaaaggacgc ctcagacggc agcgccggcc ggtctcctcg cggacttccc
     6061 gcagggcggc gtcttcggcg gtctcgcctt tttccagatg ccccttggga aacgtccaga
     6121 cgacgcggcc ttccaggttg cgcaccttca ccatcaggag tttcccgtcg ccgagcacga
     6181 ggccgccggc cgacacctcc acagcttccc ccgggacttt tttccgccgg cccgcgggcg
     6241 tctttttcat ttctgttcca ggccccggcg gagtaactgg tcggggaacc ccgcaccgct
     6301 ggaaagcacc agctggatgc cgtcctgcac cgacaaggtc gtggccagaa ctttggattc
     6361 cggcaccacc agcgtgttgc cgagataaaa atggttggtg gggacgtaca ccaccgcgtg
     6421 tttctgagag gcgccgtcga cggtcaacac ggtctgtccg gtgacgaacc ccagcgaaaa
     6481 actgcccgga tgcggatatt ccaccagcac cacgtttcgg aaagatttct ggttctccgg
     6541 cgagaacgtc tcggtgagcg ctttcacggt cttgtagatg acccgcacgc cgggcacgcg
     6601 gaggagggcc tcctcgaaaa ggtcgaacat ctgtttgaac agaaggttcg acatgagcat
     6661 gcccaccagg aggatgagtc ccacggtcat gacgatcccg agaccgggga tgtggaaccc
     6721 catcagtccc cgcaccatgc cgcccagaag gccgtcgagc gtgccgatga gccagaacac
     6781 ggtaacgacc gtgaacgcca cggggatgag gacgaagagg ccggtcagga aataccgttt
     6841 cagcgtcttc agtttcgtct catccacggg acggctccgg cgggatcctg ttccaatcgg
     6901 tggcctgttc gtaagcggcc gcggcggaaa aaacttgttc ttccccgaac ggtttcccca
     6961 ggatctgcag gccgaggggc agccccttgg acgaaaaccc gcagggcatg gaaaggcccg
     7021 gcagccccgc gaggttgcag gagatggtga agatgtcgga aaggtacatc cgcagaggat
     7081 cgtcggtgcg ttccccgatg cggaacgccg gggtgggcgc ggtgggagtc aggatcaggt
     7141 ccaccttttg aaaagcatcg tcgaagtcgc gcttgatgag ggtgcgcacc ttctgggcct
     7201 tgaggtaata ggcgtcgtag tatccggagg agagggcgta ggtaccgagc atgatgcgac
     7261 gtttcacttc cggcccgaat ccccgggcgc gagagagttc gtactgttcc aaaagggtgg
     7321 tcgcctcggg ggcgcgggtg ccgtaacgca cgccgtcgaa ccgggccagg ttggcgctgg
     7381 cctccgacgg ggccaggatg taatacaccg acaccgccgc gtcggtgtgg ggcagtgaaa
     7441 tcttctcgaa ccgcatcccc gccttttcca gcacccggat ggattcctcc accgcccgtt
     7501 cgacttccgg gtccagcccg ccgcggaaat attcctccgg caggccggcg gtcagccctt
     7561 tgacgccggc ggtcagcgcc gggagataat ccggcacgga gcggttcacg gaggtggaat
     7621 cgtgcgggtc gtgcccggcg atgacgccca gcagggcggc ggcgtccgcg atgttccagg
     7681 cgaacgggcc gatctggtcg agagaagagg caaaggcgat gagaccgaac ctcgacacca
     7741 aaccgtaggt cggtttcatg ccgaccacgc cgcaacaggt ggcgggctgg cggatggacc
     7801 cgccggtgtc ggaaccgaga gccaacggcg ccaggcgggc ggccacggcc gcggcgctgc
     7861 cgccggagga cccgccgggg atgtagtccg tgttccaggg gtttcgggtg gtgaagaacg
     7921 ccgagttttc cgtggaagaa cccatggcga actcgtcgag gttggtcttg ccgatgaaaa
     7981 cggcgccggc cgcgcgcaac ttggcgatga cggtggcgtc gtagttggcg gtaaaatttt
     8041 ccaggatctt ggaggaacag gtggtccggg tgccgcggac gcacatgttg tccttcacgg
     8101 ccacgggcac gccggccagg ggaccggcct tctcgccgcg gcgcacttgc tcgtccaccg
     8161 cccgggcctg ggccacggcg gtatctttca agacggaaag gaacgctttg acgcgcgggt
     8221 cccacgcggc gatccgcgcc aagaaggcgt tggtcacttc ctcggcggac accttgcggg
     8281 cgccgacctg ttcggcgatc tgccgggcgg aaagctcgtg gaggttcatg gttcgatgac
     8341 ttttttcact ttgaaatagg aattttcccg ttcgggggcg ttggccagga tggactccac
     8401 gtcctcccag cggcggacct cgtccggacg ccacacgttg gacagaggcg ccacatgcga
     8461 ggtgggcggc acgtccccgg tggcgtgttt ctccaggttc ttcatgtagt ccaggatgcg
     8521 ctccaactga ccgcggagcc gttccctttc ctcgtcggaa agggagagcc gcgccaggcg
     8581 cgccacgtgc ttaacgtctt tttccgtcaa cgccatcgtc tgtctccagg cagaaatttt
     8641 tcacccaatc atacatgatt cgacgcgcct tctccaaccg gtcgagcggc aagcgctcgt
     8701 cggaggcgtg catgaggaga tattcctcct ccgtcagcac caacggcacc aggccgtaac
     8761 accggacccc cttggcgcga aagaaccgtg aatccgtggc gccgggcatc aggaacggcg
     8821 ccacgacggc cttctcatcg tggcggcgga cggcccgcac cagaccgtcg tagagcggag
     8881 accggtagtc ccggtaatcg ctggtgacgc gcgtctgcac cgtgacctcg atccggacgc
     8941 ggtcgtcgtc cacggcgcgg cggagtttcg ccagaaggtc ttgttcgtcc tccccgggca
     9001 ggagccggca gtccagcgtg gccaccgccc ggtcgggaat gacgttgacc gtgggggtgg
     9061 cggcggcgcc gctccaggac tggaaggagg taaccgacag ggagttgtgc cgcaccgcct
     9121 ccaacagcgg cgattttcgc ggatgcggca ccgcgcgctc cagatcgcgc accggttcgg
     9181 gcgtgcggcc tccgaaacgg atgaaggtct gttccatccg ggccagggct ttgcgcagga
     9241 catcgatggg gttgtcggtg ttcggcaccg agccgtgccc ggaccgcccg ttggccgtca
     9301 gtttcagcca cagcaccttt ttctcgtcca cggcgcaggc gtacacgggg acggggtcgt
     9361 aggaaaagac atcgatgagt ccgaaaccgc cctcgtcgat cacgaaaccg ggcttcatcc
     9421 tgtcccaatg gttctccacc atccactggg aaccccagcg gccgccggcc tcctcgtcgg
     9481 ccacggccag gagaaggacg tcccggttca gacggacgtc ctcccgtttc aactggaaga
     9541 gggtcatgag gtgcagagcg ccggtgattt tactgtcgag ggcgccgcgg ccccagagat
     9601 agccgtcgcg ttccttggcg gcgaagggat cgatgctcca ttcggcggga tcggcgggca
     9661 ccacgtccat gtggtggatg agcaacaagg gcggttgcgt cggtttttcc gccttcaacc
     9721 gggccagcag gttcgcccgg ccgggcgacg acacgaaaat ctcgctgggg atgtcctccc
     9781 gttccaacaa gccgcgcaga tactccgcgg cgcggagttc ccggcccggc gggttggcgg
     9841 tatcgatgcg gacgtatcct ttcaacacgg cgacggtctc gtcggtcaac gggtccgggg
     9901 ctttccggtt ttgcgcccac agaggccacg cggcgaaacc gacgaggccg aggaacaggg
     9961 tcgcggattt taaagtttcc cgcatgttca tcctttcagg atctcgaacg aggcgtagcg
    10021 cgccacgatg gttttgcgca tgccgctgtc gaaatccacc gtgaccttga ggttttcccc
    10081 cgcgccggac cggcccgcga tgcggccctc cccgaaagaa ggatgtctca cgcgcatgcc
    10141 cacctgcagg ggccggagcc cgccgggggt cgggagggcg gcgcggttga gttcgtcggg
    10201 atcgaacgcg ccgaccaccg cgccgtattc ctcgctgcgg cccggcgcgg gcgccggcac
    10261 ggaagccagc tccgcctcct gcacgaaacg ggacggcacg ttccaatggg acttcccgta
    10321 gattttgcgc gaagcggcgg cggtgaggaa aagatggcgc cgggcgcgcg tcatccccac
    10381 ataacagagg cggcgttcct cctccagctc ttcctcgtcg aaagccgact cgccgatggg
    10441 gaacaacccc tcctccatgc cggtcatgaa aacggccgga aattccagcc ctttggccag
    10501 gtggacggtc ataaacgtcg ccgagccgtc ggcgtcgttc cagccgtcga tgtccgaaac
    10561 gagggacacc cgctccaaaa acagcgccgt ggaccgatcc tcggaactct cctcgaactc
    10621 cttggcggcg ttcaccagct cctggaggtt gtcgagccgg taggcggcct cggggtcgtc
    10681 atcggtctgt tcctgccaat ggtcccaaca gccggtctcc tcgaggacgg cctggaccat
    10741 ggcggcggcg ccggtgacag ccattttgac cgccagcggc cggaggatgt ccaaaaattt
    10801 ctgcaggttg ccgcgcgcgg cgctggtcag gtccggcacc cgcgccgact gcacgatgga
    10861 ctcgtaaaag gagacgccgt gggccaacgc gtatttttcc agagcctgca cggtggtctt
    10921 gccgatgccg cggggcggga cgttaaggat gcgtttcagg ctgacggagt ccgcggggtt
    10981 caagagaacg cgcagaaaag ccagcacgtc ccgcacctcc gcccgttcgt aaaatcggac
    11041 cccgcccaca acgctgtacg ggatgttctc ccggcgaaaa gcgtcctcga gcacgcgcga
    11101 ctgggcgttg gtgcgataga acacggccac gtcgcgccgg acgacctcgc cggccgccaa
    11161 caggcgcgcg gtctcccgcg ccacggcccg cgcctcctcc agttcgttgg cgaactcctg
    11221 aaaccgaacc ggcaggccgc cggcctggtc cgtccaaagc cgtttctcct tgcggaactt
    11281 attgcgccgg atgatcttcc aggcggcggc gaggatggtc tcggtggacc ggtaattctg
    11341 ctccagcttg acgacggcgg cgccgggata gtcccgctcg aattccagga tgttgcgcac
    11401 attggcgccg cgccaggaat aaatggactg gtcgtcgtcg cccaccacgc agatgttctg
    11461 ctccgaattc accaggccca tgagcatgac gtactgggcc atgttggtgt cctggtattc
    11521 gtccaccagg aggtagcgga agagcgactg gtagcggccc ttcacctcgg gcatactttg
    11581 aagcatctcg cccgggacct tgagcaggtc gccgaagtca aaggcgccgt aggccttctt
    11641 gcgctcctcg tagagcgcga tgaccggtcc ggggtcgaag tccagcttgc aggtgatgcg
    11701 ggtatagtcc tgcaaagtat ccttggccat ggcgatcagc caggccacgg tgctgtccgt
    11761 gcccttggag gtcttgagct tgaggtcgcg gatgcacttg ttcacgagcg agcgctggtc
    11821 ttcgctgtcg tagatgatga acgagcgcgg gtagcccagg cgcccggcct cgtggcgcag
    11881 gaaccaggcc ccgaaggcgt ggaaggtgcc agcccacatg ccggggtagc cgtggcccag
    11941 catgcgctcc aggcgctcct t
//
LOCUS       DAOTFD010000061        11857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_3793_length_11857_cov_4.156075, whole genome shotgun sequence.
ACCESSION   DAOTFD010000061 DAOTFD010000000
VERSION     DAOTFD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_11857_cov_4.156075"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="P5079_05310"
     CDS             <1..409
                     /locus_tag="P5079_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29441.1"
                     /translation="DFGFLFLYNYHFTPRQVSVTLPFPGGPRNFHLPSKGRLWMEPLS
                     AMILPLNLPLAKDVRFLHATAEPLDLRLGRKKMEVVFSSLPGRTVEGALALPKKPRAV
                     KAGARKLPVRWNRGVLSLSFKAETPETALTVAL"
     gene            441..2888
                     /locus_tag="P5079_05315"
     CDS             441..2888
                     /locus_tag="P5079_05315"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HRY29442.1"
                     /translation="MKAPRFPFKTPYGSFSDDGKSFIITRPDTPRPWVNIMSTGTYGV
                     AVSQGGSGYSWLNHASLNRITRWNQDLIRDDWGRFIYVRDDQSGKFWSVGWQPVKAAM
                     DKYECIHGVGYTIINSEKDGLQTQWLVFVPHDEPLEIWRLRVKNLSRKPRRLSLWTYM
                     EWNLGESPDWHREFHRTFIETAFRDDKIILAKKRLSPLKNAKGQGWNRTWDHCAWHAS
                     SVPARDLCGDKEAFLGRYGSLEAPAALQTGHYVGKTTHKWDDGIASLCVPLSLKAGEE
                     RSLLFTVGAADTDPQALIKARQFMKFDEVDRVWHRTETFWDKYLSAFPVSTPDPAFNL
                     LTNTWLKYQALSARLWGRTAYYQTGGAYGFRDQLQDSQVFLPLEPDRTREQIRLHAAH
                     QFSDGTVYHWWHPLSEEGHPSNYSDDLLWLPFVMVNYLKETAQWSLLWENVPFAKRPQ
                     EFRSHASSLYDHAVRAIEKSLTRFSSRGLPLIGEGDWNDGLSATGTAWKGESVWMGHF
                     LYGVLNDFAELIDHAVGNKALPAKEKSRARTYRARAARLKKALNRHAWDGQWFWGATT
                     DDGKLLGSHKSKEGRIWLNCQTWALLKGVVDAPARKKAMLAALDKHLYGPHGPLLLAP
                     AYSVPDETVGYLTRYSPTTRENGGIYFHAAVWALQMECHLGRARKAWDLYKRMNPVLR
                     SAADPELYRCEGYVTPGNVDGPGSPTPGRGGWTWYTGSAAWLYRISTEWFLGIRPEWD
                     GLRVRPCLPPEWDKAEATRLFRGGIYRIRFQRNSRLAAGAQRILLNGEKIAGDVLPAT
                     HRRHNEVTVLVGPAPRT"
     gene            2961..4007
                     /locus_tag="P5079_05320"
     CDS             2961..4007
                     /locus_tag="P5079_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29443.1"
                     /translation="MSNSEKSILVVDDDATILAACADSLMIDGYQVDIAESGEKALQM
                     IPKKHYDMVVTDLMMLEVDGLGVLQAAKKNSPQTEVILLTAYGRVDSAVEAMRLGAYD
                     YLTKPFDPHKLDVSIKRAFEHQTLLRELSGLKEILRLYDATKTLSTIRDQQELLETLA
                     KFAAEITNSSGVSIMTVSPDGKELVVTATHGTRHGILLGKSIPLNRERVSRLNSDALK
                     TLDSLDVRKYFQMESVPGFSDITSSLSIPIFFKDRLEAIMNLSRTGAENLPFREDELR
                     LITIFTAQAGYAIENSRLMESLQTKSKQDVFSWLLETCQKIQSLPAAQSLPPSEKEKI
                     EATIEQCRHALGKS"
     gene            complement(4115..5533)
                     /locus_tag="P5079_05325"
     CDS             complement(4115..5533)
                     /locus_tag="P5079_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY29444.1"
                     /translation="MKIGTKVSLMTSLLIVLIVTGISGVLFYSEKKVLVAEIQNRHLT
                     LLNTFRQAAEESLATKDDILLLNYMKLLRKTEGLSAAMVLKRDGAVLAHTDTARWGMA
                     LTDAATREILSADGPRQRMASDEQGRPVMDMVLPVGPASPEVLVRIAFSKEFIDGEIR
                     AAMRQHEKRILLIAAAALLFGLGLSLALAQTFLRPIRRLTEATAILGKGKLDHTITVD
                     SKDELGALARDFNVMAARLLELDQMKKDFVSSVTHEFRSPLHSTGMYLGLFQKGGAGE
                     LTEKQLSYLKVIEQNTVRLSRFVDDLLDTAKIERGKLEVVKQEFSLAELLKDVQAMFQ
                     PNADQRKISYELPTAEGLPKLMGDPDRTRQILTNLLSNAFKFTPEGGRIAVGVAVKDG
                     FAAVSVQDSGLGIPADQLEKIFDKFEQVKGAPRLTGQPKGTGLGLAIVKGLVELQGGK
                     ISVTSELQKGSCFTFTVPIVNS"
     gene            complement(5544..7967)
                     /locus_tag="P5079_05330"
     CDS             complement(5544..7967)
                     /locus_tag="P5079_05330"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29445.1"
                     /translation="MWEDAPEGIRERTLRFSLDVIGLARGLMADPVARRLADQVLRSA
                     TSIGANVEEAHGSETRREFARGMTIAKKEARETFYWLTLISKSGLLNGRSIDAMLKES
                     DELLRILSVIVRKTVKVLIFTLFVFRISSLVSAASTSFDFLNIGAGARVMGMGGAGAA
                     TSGDSLSAAWNPALVGALDRHDVSFSHVRWLEEFRHSAFAYGGPLSARWNAGVHALYF
                     SRADIQGYDDGGQATDRLDVSDLAAAFTVAHDIGPWFGGGLGRTHVGVSAKYLREDLD
                     VTDAAGYAFDVGLKATPYNRSQKWLVDRVQVGAGLLNWGPRIKFDREKEDLPLIYRAG
                     VSVRGWAKSLTVSTDYEKPRADPGSVRFGAEYLVFNSLALRGGYRWALKDNLRTLQNG
                     FHVGMGVQLGNAWFDYAYVPFLKLDDTHFLTVRFAFGSNVRLEAVEQRLAEHYLAAQR
                     AYVRKDYAAAQKEIDRVLLVDPAHAGARRLAEENQKQMKGLAVEKQLATGKRKLAIGD
                     YAVAMESFQSVLNVFPQHEEASRLLAESRTHVETQKVHRRDALIKQGEDFYEQGNYSA
                     AIDLWEKVLLLDPANAAVTASLEKARAKQEEQKLAAAAAEERKRLEEGWAFFKDKKYE
                     KAQRVAEEALKKNPTSEGAGVLLSQANGRLADGLLVEGRRQFQAGQMQKAFAVLKQAN
                     ELDADNQIVLDLLQKTRESLLRTGREEALNLYEKQKYDEALEKLEFLLTVDPSSTDLA
                     RYRDEINRKRQEGRRAEAERLNREALKAYDEGDLSRAVKNWKKVLELVPDHANAKRNL
                     ERALQQLKE"
     gene            complement(7997..9283)
                     /locus_tag="P5079_05335"
     CDS             complement(7997..9283)
                     /locus_tag="P5079_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY29446.1"
                     /translation="MRFYQGDPASGGTLFHTSTITLPLTAGGSAVVATTWSASSAGSY
                     DLYVQVDGGGLIDESDETNNKAFKTVSVVQLTPRLTQSTPTDGALGIFPTAPLVFQFN
                     KSMSAATLTGAFSLRAVLDNEGSSVNELVTGTLAYDDSRKQAVFTPSAFLKDNHRYEA
                     TWSAAAQDALGFPMTPVSLSFQTAASAQSRNVVLASDRLTKVVFEPGAVASSYYVQVD
                     VSPPANAAVESAANKTLQDKDAFSVPVSATLRRLVLYQGGAAVSPSFAADVRLTIPYA
                     DDGRGYVAGTNPPVKEENLSMYWLNETDGLWVRLPESVVDAVQNTVTSRVPHFSYFVL
                     MGLGASDLSRAYAYPVPFRPNTGLGHARIKFTGLSPQCVIRIYGVSGEWVKTIEERDG
                     DGFNDTWDASDVPSGTYLYIIDNGAERKTGQLVIVK"
     regulatory      10280..10451
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            10542..>11857
                     /locus_tag="P5079_05340"
     CDS             10542..>11857
                     /locus_tag="P5079_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29447.1"
                     /translation="MPSGFKKYLIPIALLLLAARVPLSGEEVIIIDPDPIIIIEPDPQ
                     PNPDPPPPPPDPEPDPPPITDPVVVLETGTVTFAFESHGNFYSEIKRFLDWMGVSYEP
                     WPLWSALREFGGECLKDRGHHSGGGWHGHGHHNNNDPPLPETETHTLDLLPLVNSAWQ
                     GGIIKFELILTCRLDEHTPDNIVLNLTYKQPVLRVSDGSVVQMPVDVRMDPSHVNTYT
                     HREFITMSLELPEGYNPREINRLTVRITQIDGQAVEPPIRSSPQNFSFTDRDWDHKPE
                     LRLKFRYSRLKPYLAHVGTGQFTVKGFMNTGEEFSTEYSQLFIRSNGHHVVCDRKGRA
                     QVVVAVTTQTAPLPLTVQEDQSGDIERERIALRNLLKTAGEGYHFGPEGTVFQQPVEI
                     TIPYDPEKLNGVPETDLKVFYWNKVEKIWEPLISRVDTVAHTVTAEV"
BASE COUNT         2425 a         3835 c         3362 g         2235 t
ORIGIN      
        1 cgatttcggg ttcctgttcc tctacaatta ccacttcacc ccgcgccagg tctcggtgac
       61 actgccgttt cccggcggcc ccaggaattt ccatctgccg tcgaaaggac gcctgtggat
      121 ggaacccctg tcggcgatga tcctgccgct gaacctccct ctcgccaagg acgtccggtt
      181 cctgcacgcc accgcggaac cgctcgacct ccgactcgga cggaaaaaga tggaggtcgt
      241 cttctcgtcg cttcccggcc gcacggtgga aggggcgctg gcccttccca aaaaaccgcg
      301 cgcggtgaag gccggcgccc ggaaactccc cgtccgctgg aaccgcggcg tcctgtccct
      361 ctcttttaaa gccgaaacgc cggaaacggc gttgacggtc gccttatgat ttgaaccacc
      421 cgaaaaaagg agaaccttcg atgaaagcac cccgtttccc gttcaaaacg ccctacggct
      481 ctttttccga cgacggaaaa agtttcatca tcacccggcc ggacacccct cgcccctggg
      541 tcaacatcat gtccaccggc acctacggcg tggccgtgtc ccagggcggt tccggctact
      601 cctggctgaa ccacgcctcc ctcaaccgca tcacccgctg gaaccaagac ctcatccgcg
      661 acgattgggg ccgcttcatc tacgtgcgcg acgaccagtc cggcaaattc tggtccgtgg
      721 gctggcaacc cgtcaaggcc gccatggata aatacgagtg catccacggc gtggggtaca
      781 ccatcatcaa ctcggagaag gacggcctcc agacgcaatg gctggtcttc gtgccgcacg
      841 acgagccgct ggagatctgg cgcctgcggg tgaaaaacct ctcacgcaaa ccccgccggc
      901 tcagcctgtg gacctatatg gaatggaacc tcggcgaatc gcccgactgg caccgcgagt
      961 tccaccgaac cttcatcgag accgctttcc gcgacgataa gatcatcctc gccaagaaac
     1021 gtctctctcc cctgaaaaac gccaagggcc agggctggaa ccgcacctgg gaccactgcg
     1081 cttggcacgc ctcgagcgtg ccggcccgcg acctttgcgg cgacaaggag gccttcctcg
     1141 gccgctacgg gtccctggag gcgcccgccg ccctccaaac cggacattac gtggggaaaa
     1201 ccacccacaa atgggacgac ggcatcgcca gcctctgcgt gccgctttct ctgaaagccg
     1261 gggaagaacg cagtcttctt ttcaccgtcg gcgccgccga caccgacccg caggccctca
     1321 tcaaggcccg gcagttcatg aagttcgacg aggtggaccg cgtgtggcac cgcaccgaga
     1381 ccttctggga caaatatctc tcggcgttcc ccgtctccac gccggacccg gcgttcaacc
     1441 tgctcaccaa cacctggctg aaataccagg cgctctcggc ccggctgtgg ggccgcaccg
     1501 cctattacca gaccggcggc gcctacgggt tccgcgacca actccaggac agccaggtgt
     1561 tccttcccct ggaaccggac cgcacgcggg aacagatccg cctgcacgcc gcgcaccagt
     1621 tttccgacgg caccgtctac cactggtggc acccgctgtc cgaagagggc catccgtcga
     1681 actattccga cgacctcctc tggctgccgt tcgtgatggt gaactacctc aaggagaccg
     1741 cccaatggtc cctgctgtgg gagaacgtgc cgttcgccaa acggccacag gaattccgca
     1801 gccacgccag ctccctctac gaccacgcgg tgcgcgccat cgaaaagtcc cttacccgtt
     1861 tcagctcccg cggcctgccc ctcatcggag aaggcgattg gaacgacggc ctctccgcca
     1921 ccggcaccgc ctggaaaggc gaaagcgtgt ggatgggtca tttcctctac ggcgtcctca
     1981 acgacttcgc cgaactcatc gaccacgcgg tgggaaacaa ggccctgccc gccaaagaaa
     2041 aaagccgcgc ccggacgtat cgcgcccgcg ccgcccgact caaaaaggcc ctcaatcgcc
     2101 atgcctggga cggtcagtgg ttctggggcg ccaccaccga cgatggaaaa ctcctcggca
     2161 gtcacaaaag caaggagggc cgcatctggc tcaactgcca gacctgggcc ctcttgaaag
     2221 gcgtggtgga cgcgccggcg aggaagaagg cgatgctcgc cgccctggac aaacatctct
     2281 acggccccca cgggccgctc ctgctcgccc ccgcctactc cgtgccggac gagaccgtcg
     2341 gctacctcac acggtattcg cccaccaccc gggagaacgg cggcatctat ttccacgccg
     2401 ccgtgtgggc cctgcagatg gaatgtcatc tcggccgggc gcgcaaggcc tgggacctct
     2461 acaaacgcat gaatccggtc ctccgctccg ccgccgatcc cgaactctat cgttgcgaag
     2521 gatacgtcac ccccggcaac gtcgacggcc ccggttcgcc cacccccggc cgcggcggct
     2581 ggacgtggta caccggctcg gcggcgtggc tctaccgcat ctccacggaa tggttcctcg
     2641 gcatccgccc ggaatgggac ggtctgcgcg tacggccctg cctgccgccc gaatgggaca
     2701 aggccgaagc cacgcgcctc ttccgcggag gcatttaccg cattcggttc caacggaaca
     2761 gccgcctggc cgccggcgcc caacgcattc tcctgaacgg ggaaaagatc gccggcgacg
     2821 tcctgccggc gacccaccgc cggcacaacg aggtcacggt gctggtggga ccggcgccca
     2881 ggacctgagt tttttggagt tcaccgcgaa acggcggatt aaaaaggagg cgctgcgcgc
     2941 gcatcggcag cggaaaaccc atgtctaatt ctgaaaaaag catcctcgtg gtggacgacg
     3001 acgccaccat tctcgccgcc tgcgccgact cgctgatgat cgacggctat caggtggaca
     3061 tcgccgaaag cggcgaaaag gccctccaga tgatccccaa gaaacactac gacatggtgg
     3121 tgaccgacct catgatgctg gaagtggacg ggttgggcgt gctgcaggcc gccaaaaaga
     3181 actcacccca gacggaagtg atcctgctca ccgcctacgg acgggtggac tccgcggtgg
     3241 aagccatgcg gctgggcgcc tacgactatc tgaccaagcc tttcgatccc cacaagctgg
     3301 acgtctccat caaacgggcc ttcgaacacc agaccctgct gcgggagctc tccggcctca
     3361 aagagatcct ccggctctac gacgccacca agacgctctc caccatccgc gaccagcaag
     3421 agctccttga gaccctggcg aagttcgccg ccgagatcac caattcctcc ggcgtctcca
     3481 tcatgaccgt ctcccccgac ggaaaagaac tcgtcgtcac cgccacccac ggcacgcggc
     3541 acggcatcct tctggggaaa tccatcccgc tgaacaggga acgcgtcagc cgcctcaact
     3601 cggacgcgct gaaaaccctg gactccctcg atgtccggaa atattttcag atggagtcgg
     3661 ttccgggctt ttccgacatc acctccagcc tctccatccc catcttcttc aaggaccggc
     3721 tggaagcgat catgaacctt tcccgcacgg gagcggagaa ccttcccttc cgggaagacg
     3781 aactccggct catcaccatc ttcacggcac aggccggata tgccatcgag aactcgcgtc
     3841 tgatggagtc cctgcagacg aaatcgaaac aggacgtttt ttcctggctg ctggagacct
     3901 gccaaaaaat ccagtccctg cccgccgccc agtccctgcc tccctcggag aaggaaaaga
     3961 tcgaggccac catcgagcag tgccgccacg ccctgggaaa aagctaatcg gccgcgccgg
     4021 ttttccctgt aaaaacacct caccgcccag gccgcaacgg cctgggtttt ttactaccgt
     4081 ttatttttgg atttgacgcc gaagacggac gtcttcagct gttgacgatg gggaccgtga
     4141 aggtgaaaca actgcctttt tgaagctcgc tcgtcactga aatctttccg ccctgcaact
     4201 ccaccaaccc cttgacgatg gccagcccca ggccggtgcc cttgggctgc cccgtcagcc
     4261 gcggcgcccc tttcacctgc tcgaacttgt cgaaaatctt ctccaactga tccgccggga
     4321 tgcccagtcc cgagtcctgg accgaaacgg ccgcaaaacc gtctttcacg gccaccccga
     4381 cggcgatccg gccgccctcc ggagtgaact tgaaggcgtt gctcaaaagg ttggtgagaa
     4441 tctgccgcgt gcggtccggg tcgcccatca gtttcggaag tccttcggcc gtggggagct
     4501 cgtaggagat cttccgctgg tccgcgttgg gctgaaacat cgcctgaaca tccttgagga
     4561 gctcggccaa ggaaaattcc tgcttgacca cctccagctt cccgcgctcg atcttggccg
     4621 tgtccaacag atcgtcgacg aaccggctca aacggaccgt gttctgctcg atcaccttca
     4681 aataggacaa ctgtttctcg gtgagctcgc cggccccgcc tttctggaac aggcccagat
     4741 acatgccggt ggagtggagg ggggaccgga attcatgcgt cacgctggag acgaaatcct
     4801 tcttcatctg gtccagttcc agaagccggg cggccatgac gttgaaatcc cgcgccaaag
     4861 cgcccagttc gtccttggaa tccaccgtga tggtgtggtc cagctttccc ttgccgagga
     4921 tggcggtggc ctcggtgagc cggcgaatgg gccggaggaa ggtctgcgcc agggccaggg
     4981 agagccccag cccgaagagg agggcggcgg ccgcgatgag caggatgcgt ttttcgtgct
     5041 ggcgcatggc ggcgcggatt tcaccgtcaa tgaattcctt ggaaaaagcg atgcgcacca
     5101 gcacctcggg cgacgccggt cccaccggca ggaccatgtc catcacgggc cggccctgct
     5161 cgtcggacgc catgcgttgc cgcgggccgt ccgccgacag aatctcccgg gtggcggcgt
     5221 ccgtcaaggc catgccccag cgggcggtgt cggtgtgggc cagcacggcg ccgtcgcgtt
     5281 tgagcaccat ggcggcgctg agcccttcgg ttttccggag gagtttcatg tagttgagga
     5341 gcaggatgtc gtctttggtg gcgagggact cctccgccgc ctggcggaag gtgttcaaca
     5401 gggtgagatg ccggttctgg atctccgcca ccagcacttt cttttccgaa taaaaaagaa
     5461 cgccgctgat cccggtcacg atgagcacga tgagaaggct cgtcatcagg ctgactttgg
     5521 tgccgatctt catggggttt cctttactcc ttcaattgct gcaacgcccg ttccaaattg
     5581 cgtttcgcgt tggcatggtc cgggaccagt tccagcacct tcttccagtt cttcaccgcc
     5641 cgggaaaggt caccttcgtc gtaggccttg agggcctctc ggttcaggcg ttccgcctcg
     5701 gcgcggcggc cttcctggcg tttgcggttg atctcgtccc gatagcgcgc aagatccgtc
     5761 gatgatggat ccaccgtcaa caaaaattcc aacttttcca gcgcctcgtc gtatttctgt
     5821 ttctcataca aattgagggc ctcttcgcgg ccggtgcgga ggagggactc ccgcgttttt
     5881 tgcagcaagt ccagcacgat ctggttgtcc gcgtcaagct cgttggcctg tttcaacacg
     5941 gcaaaagcct tctgcatctg cccggcctgg aactgccggc ggccttccac caaaagcccg
     6001 tccgccaacc ggccgttggc ctgcgagagc aggaccccgg ctccctcgct ggtgggattc
     6061 tttttaagcg cctcctcggc cacgcgttgc gccttttcgt atttcttgtc cttgaaaaac
     6121 gcccagccct cttccaggcg tttccgttcc tcggcggcgg ccgccgccag cttctgttcc
     6181 tcctgtttgg cgcgcgcttt ttccagagaa gccgtcaccg ccgcgttggc gggatccaag
     6241 aggagcacct tctcccacaa gtcgatggcc gcggagtagt tgccctgctc ataaaaatcc
     6301 tcgccctgct tgatgagggc gtcccgacgg tggaccttct gggtttccac atgcgtccgg
     6361 ctctccgcga ggagccggga ggcctcctcg tgttgaggga aaacgttcag gaccgactgg
     6421 aagctctcca tcgccaccgc gtaatcgccg atggccaatt tccgtttgcc cgtggccaac
     6481 tgcttttcca ccgcgagccc tttcatttgt ttctggttct cctccgccaa ccggcgggcg
     6541 ccggcgtggg ccggatccac cagcaacacc cggtcgatct ctttctgcgc ggcggcatag
     6601 tccttgcgga cataggcccg ctgggccgcc aggtaatgtt cggccaaccg ctgttccacg
     6661 gcctcaagac ggacgttcga gccgaacgcg aaccgtacgg tgagaaaatg ggtgtcgtcc
     6721 agtttcagga agggcacata ggcgtaatcg aaccaggcgt tgccgagttg gacgcccatc
     6781 cccacatgga aaccgttttg cagggtgcga aggttgtctt tcagggccca gcgatagccc
     6841 ccgcgcagcg ccagcgagtt gaacaccagg tattcggcgc cgaaccggac ggagcccgga
     6901 tcggcccggg gcttttcata gtcggtggaa acggtgaggc tcttcgccca gccgcgcacc
     6961 gaaacgcccg cccggtaaat caacggaagg tcctcctttt cccggtcgaa ctttatccgc
     7021 ggcccccagt tcaacaagcc ggcgccgacc tgaacccggt cgacgagcca tttctgcgaa
     7081 cggttgtagg gggtggcctt aagtcccacg tcgaaggcgt atcccgcggc gtcggtaacg
     7141 tccaaatcct cgcgcagata tttggcggaa acacccacgt gggtccgtcc cagcccgccg
     7201 ccgaaccacg gcccgatgtc gtgggccacg gtgaacgccg cggccaggtc cgaaacgtcc
     7261 aggcggtccg tggcctggcc gccgtcgtcg tatccctgaa tgtcggcgcg tgaaaaatag
     7321 agcgcatgga cgcccgcgtt ccaacgggcc gacaggggcc cgccgtaggc aaacgcgctg
     7381 tgccggaact cctccagcca gcgaacgtgg gaaaacgaca cgtcgtggcg gtcgagcgcc
     7441 ccgaccaaag ccgggttcca tgcggcggac aatgaatctc cggaggtggc cgctcccgcg
     7501 ccgcccatgc ccatgacccg cgcccccgcg ccgatgttga gaaaatcgaa agacgtagat
     7561 gccgcagata caagagacga aatacgaaat acgaaaaggg tgaaaatcaa aaccttaacg
     7621 gtcttccgga caatgacgct caatatgcgc aggagctcat cggattcttt cagcatggca
     7681 tcgatcgaac gaccgttaag caagccgctt ttggaaatca gcgtcagcca ataaaatgtc
     7741 tcgcgggcct ccttcttagc gattgtcatg ccgcgagcga actccctccg ggtttccgag
     7801 ccatgggcct cctccacgtt cgcgccaatg gaggtcgccg atcgcaacac ttgatcggcc
     7861 aaccgtcgag cgacgggatc ggccatcagc cctcgcgcca gtcctataac atcgagcgaa
     7921 aaacgcaatg tcctctcccg tatcccctcc ggggcgtctt cccacatttc gtattttcta
     7981 tctcgtgttt tgtatctcac ttaacgatca ccagctggcc cgttttccgt tcggcgccgt
     8041 tgtcgatgat gtagagatac gtgccgctcg gcacgtcgga agcgtcccag gtgtcgttga
     8101 acccgtcgcc gtcgcgctcc tcgatcgttt tgacccattc cccggaaaca ccgtagatcc
     8161 ggatgacgca ttggggggaa agtccggtga atttgatccg cgcatggccg agtccggtgt
     8221 tgggccggaa cggcacggga taggcatagg cgcgggacag atcggaggcg ccgagtccca
     8281 tgagcacgaa gtaggaaaaa tgcggaacgc gcgaggtgac ggtgttctgg acggcgtcca
     8341 caacggattc cggcaggcgc acccagagac cgtcggtctc attcagccag tacatggaca
     8401 gattctcttc cttgaccggc gggttcgtgc ccgccacata gcctcgaccg tcgtcggcat
     8461 aagggatggt caaccggaca tccgcggcaa acgaaggaga aaccgccgcc ccgccctggt
     8521 aaagcacaag gcgacgcaat gtggcgctca ccggaacgga gaaagcgtct ttgtcctgta
     8581 gtgttttgtt cgccgcggac tcgacggcgg cgtttgcggg aggagaaaca tccacctgaa
     8641 cataatagga agaagccacc gcaccaggct cgaatacaac cttcgtcaac cgatcggagg
     8701 cgagcaccac gttgcgactc tgcgcactgg ccgccgtttg aaaagacagg gaaacgggcg
     8761 tcatgggaaa ccccaaagcg tcctgcgccg cagcagacca tgtggcctcg tagcgatggt
     8821 tgtctttcag gaaagccgac ggtgtaaaca ccgcctgttt tctggaatcg tcgtaggcga
     8881 gagtccccgt taccaattcg ttgacagaac tcccttcgtt gtcgaggacg gcccgcaggg
     8941 aaaacgcgcc cgtcagcgtg gcggcggaca tggatttgtt gaattggaag acgagcggag
     9001 cggtggggaa aatccccaac gcgccgtcgg tcggcgtgga ttgcgtcaat cgcggcgtca
     9061 gttgcacgac agaaacggtt ttgaaggcct tgttgttggt ctcgtccgat tcgtcgatga
     9121 ggccgccgcc gtccacctgc acatagaggt cgtaggatcc cgccgatgag gcgctccagg
     9181 tggtggccac cacggccgaa ccgccggcgg tcagcggcaa ggtgatggtt gaggtgtgga
     9241 acaaagtccc gccggaggcc gggtcgccct gataaaaccg cacgacggtg tcctggggac
     9301 gataagtccg ataatagagt gtataactcg aaggtgaaac aagccaattg gggcccacgg
     9361 tggaacggta aaaaactgtc gagtttttgg ctacccccgt atcagcccat atcccatatc
     9421 cattggtatc atccaaatca ctttcaacac aaagccaata cgttgaaccc gctgtcaatt
     9481 caagtggcgt tgtcaaagga atatcatgcc attgcggagt tgaaatgtcc gaataatttg
     9541 tattgaaatt tgccgtcgca agaacagctc cgccgaccgc cgtgggccga tcattccccg
     9601 catcatttct gatattaact cgaatagaat cgccgagaat ccctttatta taaatttgga
     9661 gacctacttg gctcaaataa aatgatgcgg tactgatcat tttttgtcca agccaccaac
     9721 ccagtggact aacaatggct ccggttgaga caacaatccc gtttgagcct gtccccactt
     9781 gggttgcatg ggcgacaatt ggcgtggaaa caacttggtc gtggtatgcg tcgccggtgt
     9841 tgcgaacggt cgccgtcaac gtgacggcca cggtgggctc gggagaggtc tcggatacct
     9901 ggatgtcgga ggattgggaa accagatccg gcaaggccgc cgagggaagc ggacaaaacg
     9961 ccgccaaact cactataatc gcgccgaaaa atcgcctcat atccaataag tatacccgca
    10021 gtgaggtctt tgacaagggg gattcgtttc cttagcccct ctatcctctc atcggtctag
    10081 ggaaaaccct caaaaacggc aaaaaaagaa caaaaaacgg ccttaattct gccgactcat
    10141 ttccgtcgac aaaatgccgg ttttcccgtt ctctgaaagt cttgacaaaa gacggttccg
    10201 gggttaaatt tagagtgatc gataaatccc tcgttgaggg accttgtcgg cggtccgttt
    10261 gaaacggatt gccggatgta ccattaaggg gcaaaccacg tatccggggt gtgccggagc
    10321 tccgtcttct cggacggacc gaaagggtgg gacgcaaagc cacggggcta aagcctgtga
    10381 tgcacaggac agctggctgg gttgccgaaa tagcgaagtt gatcctgggc tatgctcgcc
    10441 gggttgccga aacaagatac gcgccgtctt tcgccgtcgt tacggcgaaa aaacggttgg
    10501 cgtatcgttc ggtgactcgg tttttgttta ggagcgtaaa aatgcccagt gggtttaaaa
    10561 agtatctgat ccccatcgcc cttctcctcc tcgccgcgag ggtccctctc agcggcgaag
    10621 aagtcattat tatcgatccc gatcccatca tcatcatcga acccgatccc cagccgaacc
    10681 ccgatccgcc tccgccgccg cccgatccgg aaccggaccc gccgcccatc accgaccccg
    10741 tggtggtgct ggaaaccggc accgtcacct tcgcgttcga aagccacggc aatttctatt
    10801 cggagatcaa acgatttctc gactggatgg gcgtgagcta cgaaccctgg cccctctggt
    10861 cggcgctgcg cgagttcggc ggcgaatgcc tcaaggaccg cggccatcat tccggcggtg
    10921 ggtggcatgg acacggccat cacaacaaca acgatccgcc cctgccggaa accgaaacgc
    10981 acaccttgga cctcctgccc cttgtcaact ccgcctggca gggagggatc atcaaatttg
    11041 aactcatcct gacttgtcgt ctggacgaac acacgcccga caacatcgtg ctgaacctga
    11101 cctacaagca gcccgtgttg agagtctccg acggcagcgt cgtccagatg ccggtggatg
    11161 tgcgaatgga cccctcccat gtgaacacct acacgcaccg agagttcatc accatgagtc
    11221 tggaattgcc ggagggatac aacccccggg agatcaaccg tctcaccgtg cgcatcaccc
    11281 agatcgacgg ccaggcggtg gaaccgccca tccgttcctc cccgcaaaat ttcagtttca
    11341 cggacaggga ctgggaccac aaaccggaac tgcggctcaa gttccgctat tcccgtctca
    11401 aaccctacct ggcccacgtg ggcaccggcc agttcaccgt caaagggttc atgaacaccg
    11461 gagaggagtt ttcgacggaa tattcccaac tcttcatccg ttccaacggg catcacgtgg
    11521 tgtgcgaccg gaaaggccgc gcccaggtgg tggtggcggt gaccacccag acagcgcccc
    11581 tgccgttgac ggttcaggaa gaccaatccg gcgacatcga acgggagcgc atcgccttac
    11641 ggaacctcct gaaaacagcg ggggaggggt atcacttcgg tccggaaggc acggtgttcc
    11701 agcaacccgt ggagatcacc attccctacg atccggagaa gctgaacggg gttcccgaaa
    11761 ccgacctgaa ggtcttttat tggaataagg tggaaaaaat ctgggagccg ctgatctcgc
    11821 gcgtggacac cgtcgcccac acggtcaccg ccgaggt
//
LOCUS       DAOTFD010000062        11360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4012_length_11360_cov_4.974878, whole genome shotgun sequence.
ACCESSION   DAOTFD010000062 DAOTFD010000000
VERSION     DAOTFD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4012_length_11360_cov_4.974878"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..632)
                     /locus_tag="P5079_05345"
     CDS             complement(111..632)
                     /locus_tag="P5079_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRY29448.1"
                     /translation="MSAVLRGRGLSGRILSSVRLARELGRIRRHKTVVFTNGCFDLLH
                     VGHLRVLNWAKRRGDILVVALNSDASVRRLKGARRPLLPLKDRAKLMASLRPVDYVTF
                     FPEDTPAALIRRLRPDVLVKGGDWSTDKIIGREHVRKIVRVPLAPGRSTSNTIETILK
                     RYGKGEGPVTGDR"
     gene            complement(629..1234)
                     /locus_tag="P5079_05350"
     CDS             complement(629..1234)
                     /locus_tag="P5079_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY29449.1"
                     /translation="MAMNRSGKMREKIVALLWESSRTLRDLASESADIERAAAAMARC
                     FRRKGKVVSFGNGGSAADAQHLAAELCGRFERERKGLPAVALTTNTSAITAIANDYDY
                     DQIFVRQVEGFVGRGDVAVAISTSGNSANVLAAAKLAKKRGAFIVAWTGRSGGKLKSV
                     ADVCLRVPSDRTARIQEGHLALLHTLCSLVEEDLFPRTKRR"
     gene            complement(1238..2614)
                     /gene="accC"
                     /locus_tag="P5079_05355"
     CDS             complement(1238..2614)
                     /gene="accC"
                     /locus_tag="P5079_05355"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRY29450.1"
                     /translation="MFKKILIANRGEIAVRIIRACRELGIATVAVHSDVDRDALHTRW
                     ADESVCIGPGPAAESYLNIPAIISAAEITGSDAIHPGYGFLSENTHFADVCETCHITF
                     IGPSKDAIRKMGDKAEARKIMENYGVPVIPGSDGCVDPNNPKLLKIAKKIGFPVIVKA
                     KAGGGGKGMRVVQDPAFLKEAILTAQNEAKSAFGNGDVYLEHYLTAPRHIEVQVLADS
                     HENVVSFPERDCSVQRRHQKLIEESPSTCIDDGLRKKMGKAARKAARAVKYCTVGTVE
                     FLYDEKSKDFYFMEMNTRIQVEHPVTEMVTGVDLIKEQIKTAAGEEIPFDTDDINIRG
                     HAIECRINAEDPANGFLPSPGKITNLILPGGPGVRCDTHIYPGYVVPTFYDSLLAKLI
                     VLTRKDRGEGRPQAIQRMERALREFHIDGVKTTIPFHREVLRNEAFLRGDVRTDFVEK
                     HLAIRTAA"
     gene            complement(2728..3174)
                     /locus_tag="P5079_05360"
     CDS             complement(2728..3174)
                     /locus_tag="P5079_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY29451.1"
                     /translation="MTDETTPSLQVKALVDFAKELELEEVVWEREGRRIAFKRRAAPP
                     APAAPAAASAVPANGNGKSKSPKTKDILSPIVGTFWRGLSKDRPPLVVEGGQVTAGQR
                     LAQVEAMKVQKDVIADVNGKISKILVQNGKPVEYGQKLFEIEVDPA"
     gene            complement(3174..3731)
                     /gene="efp"
                     /locus_tag="P5079_05365"
     CDS             complement(3174..3731)
                     /gene="efp"
                     /locus_tag="P5079_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY29452.1"
                     /translation="MIDTADIGNGTVFEHDGAVYQVVWFQHHKPGKGGAMLRVKLRNM
                     RTGATIERTFKSGERFKEVSLTRRPKQFLYAEGDNFTFMDMETYDQITVSRAQLGDSA
                     RFLSENMEVQAAYIDEEFLGLDLPASVTLKVTSTVPGVKGDSVSNMVKPATLENGMEI
                     QVPLFVKEGDAIRVDTRTSEYVERL"
     gene            complement(3741..4844)
                     /locus_tag="P5079_05370"
     CDS             complement(3741..4844)
                     /locus_tag="P5079_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRY29453.1"
                     /translation="MTLQMEKRVSVGRPTSEIFRRRRRALSELLQRKKWDAYFFSGMA
                     DLYYLTGFMADGFYGLFTAEGETWLFASALLAGQVRDSTQGCRLVVGRRLSKALRSLM
                     GKHGLKRVGFDSEQLMYRLGAILVREGLRPGENPAAELRAVKAEEELACVRRACQLTA
                     DGIEFAKKRLKPGMTEKGLAAELEMFFYRRGADKAAFDLVVAMGPHTALPHHLPGPSR
                     LVKNQPVILDIGCMVGGYRADLTRTLYYGKIFPLFRRVYRTVQAAQHEGIARVKPGMT
                     GGRIDAATRGVISRAGYGRYFIHSTGHGVGLDIHEAPWIRPKSGSVLSPGMVLTIEPG
                     IYLPGRFGVRIEDTLRVTDTGSEILTRPSKGKM"
     gene            complement(4906..5997)
                     /gene="aroB"
                     /locus_tag="P5079_05375"
     CDS             complement(4906..5997)
                     /gene="aroB"
                     /locus_tag="P5079_05375"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRY29454.1"
                     /translation="MKTVPVKLFAGRGYFIKVGAPLRDLGRNLRPFGLSRTALVVSDR
                     PVARRYAPVLLRGLRQAGFRPALAVVPSGEAAKNLDTVRRLYREGVTARLDRKSAVVA
                     LGGGVVGDMAGFMAATYLRGLPLIQCPTTLLSMVDSSIGGKTGVDLPEGKNLVGAFWQ
                     PKLVWMDLSVLKTLPPREWRTGIAEVVKYGVIADPDILALLEKVDLAVFRRRPDLVED
                     IVARSARIKARVVAQDENDTRGQREMLNLGHTFGHAIEAVTRYGRYTHGEAIAVGMCA
                     AARLAGRMGIFSKENVPRLEALLKRWGLPCRVRRPLPRRKILAAMARDKKAYAGRFRF
                     VVPVRWGEAKSLSDFPRSALDKVLTEIGI"
     gene            complement(6292..6795)
                     /locus_tag="P5079_05380"
     CDS             complement(6292..6795)
                     /locus_tag="P5079_05380"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRY29455.1"
                     /translation="MNIVLTGFMGSGKSAVGKILADRLKWTFYDTDVLIEKEAGVAIS
                     TIFAKKGEPAFRDMETRTVQLLGLLDKCVIATGGGIPLRPQNMDELERNGKVFFLRAR
                     PETLLERLRDGIDTRPLLKGKDPLLAVEELLRSRQKAYARCHHVIDTDGLSAEEVAEK
                     IIGLMFL"
     gene            complement(6829..7401)
                     /locus_tag="P5079_05385"
     CDS             complement(6829..7401)
                     /locus_tag="P5079_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY29456.1"
                     /translation="MPTGPRIQTHRLELIAGNRDIVQAEIGDRAAFARLLDAEIPAGW
                     PLFSNDEQTRSFALRFYETSPDADGWCKWYFLLKDEPFGRRVAVGSGGFWGPPSADGT
                     VEFGYAILSDYQGRGYATEAVNGLVGWAFGRPQVSRVIADTFRQRPAAIRVLEKNRFV
                     FVGPGAKPGTVRYELPRAVFDDVWQRHSHL"
     gene            complement(7417..8580)
                     /gene="aroC"
                     /locus_tag="P5079_05390"
     CDS             complement(7417..8580)
                     /gene="aroC"
                     /locus_tag="P5079_05390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRY29457.1"
                     /translation="MIRYLTAGESHGKCLTAIVEGVPAGLKITEDFINHQLSRRQYSF
                     GRGERLQYIEKDKVRIASGVRWGETLGSPIALVVENKDWENWEKIMSLSEADRSEKFR
                     LVRPRPGHADLAGVLKFDRRDTRDILERASARETAARTAVGTVCRRLLEEFGIKIYSY
                     VTEIGGIVAHLKGLSVEAAFELAEKSPVRCPDKHAEEKIVDAIKKAKDEGDTLGGIYT
                     VIVTGCPPGLGSHTQWDLKLDALLAQAIVSIQAHKGVEFNHGFEMTRKRGSDLHDEIF
                     HDAARGFYRKTNNAGGVEGGMTNGEPIVLQAAVKPLASLRKPLKSVNILTKEEMSAEI
                     VRSDVCPVASAAIVGEAVAATVLANAVKEKFGGDSVHEMKDNHARYLDHLRNF"
     gene            complement(8577..9518)
                     /locus_tag="P5079_05395"
     CDS             complement(8577..9518)
                     /locus_tag="P5079_05395"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRY29458.1"
                     /translation="MGKLDLRKIFENVSEDTRKLGVVGYPLGHTFSPAMHTAAIKALG
                     LRAEYKAIPVEPDQWDDFLRQARELPLFGFNVTVPYKEKAASLCGSKLDRVAIFLGAA
                     NTVLNTNEGWRGFNTDAEGFSRDLEELEISVDGKNVVLLGAGGASRAVYGSFYERREK
                     PKSIFVYDCDVAKATGMLKSFKKATGGWEGQEIDTTLPAFPLPMTQISNAEELRERIG
                     QSHLLINATPVGLKPGESPVPTEWLHGGLTVYDLIYHHETELVREARKKGGRAFGGLG
                     MLVRQGARSFEIWFDQKPDVDVMMKTVENELKERNAQ"
     gene            complement(9634..11013)
                     /locus_tag="P5079_05400"
     CDS             complement(9634..11013)
                     /locus_tag="P5079_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRY29459.1"
                     /translation="MRRRSPPIRPNKDGLWGEGKRIFESLRVPSGWQVEIYFQRERSR
                     EFAWSAGRPDDTVSSWSQGMGVRAVHRGRQGFASACEISSGAARRLWDQARAAAAFSP
                     ADARRTFYAPRPGEDEPAAEPGDDDLFRGSPGEVRDRLAFWEKRALAKDRRLKKVLKL
                     SLSETRGESALFSSMGVAVREPYGDASFSAEVLGEWKKDVQVGWDWSASRSRQSLDVE
                     RVLAGACGQAVGAFGAKALFSGTWPVVFHPKAGVEFLELLSDALGADAVQHGRSFFVG
                     RRGKRVGSSAVTLVDDGRYPGGLATARFDDEGCPTRETATVSGGVLREFLYDHYTARK
                     ENRLSTGNGNRSGVDGPPSPSASNFYLKPGGVPAAELMRDTARAFVVEDVIGMHMADG
                     VSGDFSVGAGGFLWRNGRRVHAVRGATLAGNLLEMLNGVDAVADDLTWYGAVGAPTFR
                     VKGLSVGGA"
     gene            complement(10979..>11360)
                     /locus_tag="P5079_05405"
     CDS             complement(10979..>11360)
                     /locus_tag="P5079_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29460.1"
                     /translation="DSYVLLYRRRWGILLFLCAILFFYSGFQNMAHQMSLEKPSDYLD
                     LRAAYRCFWTQQYKASIARCQEILKRDKNDVHAWSLLGASWSALGRKDQARKAWEQVL
                     RIDPEHPAGKSVLFEKKEPAHPSQ"
BASE COUNT         2091 a         3910 c         3209 g         2150 t
ORIGIN      
        1 cccgcaggcc ctcgcgggcg cgatttaaat tggcgtccaa gacgcggaac acccgcggcg
       61 ggtcggggtt cgggtgtcgc cggacaaccg cgtttttccg ttttctcggg tcaccggtca
      121 ccggttaccg gtccctcacc ctttccatac ctcttcaaaa tcgtttcaat cgtgttgctc
      181 gtcgagcggc ccggcgccag cggcacccgc acgatctttc ggacatgttc ccggccgatg
      241 attttatccg tcgaccaatc gccccccttc accagcacat cgggccgcag gcggcggatg
      301 agggccgccg gggtatcctc cggaaaaaac gtcacataat ccacgggccg gagagaagcc
      361 atcagtttcg cccggtcttt caacggcaac aacggccggc gcgcgccctt caaacgacgc
      421 acggaagcgt cgctgttgag cgccaccacc aggatgtctc cccggcgctt ggcccagttc
      481 aacacgcgca gatgtcccac gtggagcaga tcgaaacagc cgttggtaaa aaccacggtc
      541 ttgtgccggc ggattctccc gagttcccgg gccaaccgta ccgacgacaa aatgcgaccg
      601 gacaggccgc gccccctcaa aacggcgctc atctcctctt tgtccggggg aaaaggtcct
      661 cttcgaccag cgaacaaagc gtgtgcaaca gcgccaaatg cccctcttgg atgcgggcgg
      721 tgcggtcgga agggacgcgg aggcagacat cggcgacgga cttcaatttt ccgccggacc
      781 ggccggtcca ggcgacgatg aaagcccccc gcttcttcgc cagcttcgcc gccgccagca
      841 cgttggcgga attcccggag gtggagatgg ccaccgccac atcgccccgg cccacgaacc
      901 cttccacctg acggacgaag atttgatcgt agtcgtaatc gttggcgatg gccgtgatgg
      961 cggaggtatt ggtggtcagc gccacggcgg gaagaccttt ccgctcccgt tcgaaccgtc
     1021 cgcacaattc cgcggccaaa tgctgggcgt cggcggcgga gccgccgttg ccgaaagaca
     1081 ccaccttgcc cttccggcgg aaacaccggg ccatcgccgc ggcggcgcgt tcgatatcgg
     1141 ccgattcgct cgccaaatcc cgcaacgtcc ggctcgactc ccacagaaga gcgacgatct
     1201 tttcccgcat tttcccggac cgattcatcg ccatggctca ggccgccgtc cggatggcca
     1261 gatgtttttc gacgaaatcg gtacgcacgt cgccgcgcag gaaggcctcg ttccgaagca
     1321 cttcccgatg aaacgggatg gtggtcttca cgccgtcgat gtgaaactcc cgcagggcgc
     1381 gttccatgcg ctgaatggcc tggggccggc cttcgccgcg gtctttccgc gtcagaacga
     1441 tcagcttggc cagcaggctg tcgtagaacg tggggaccac gtatcccgga taaatgtgcg
     1501 tgtcgcagcg aacccccggg ccgccgggca ggatcaggtt ggtgatcttc cccggagaag
     1561 gcaagaaacc gttggccggg tcctcggcgt tgatgcggca ttcgatggcg tgaccgcgga
     1621 tattgatgtc gtcggtgtcg aaggggatct cctcgccggc ggcggtcttg atctgttcct
     1681 tgatgaggtc cacgccggtg accatctccg tgaccggatg ttccacctgg atgcgcgtgt
     1741 tcatctccat gaaataaaag tccttggact tttcgtcata caggaactcc accgtgccca
     1801 cggtgcaata tttcacggcg cgggcggcct tgcgggcggc cttgcccatc tttttacgaa
     1861 ggccgtcgtc aatacaggtg gacggcgact cctcgatgag tttctggtgc cggcgttgca
     1921 cggaacagtc gcgttccgga aagctgacca cgttctcgtg ggaatccgcc agcacctgca
     1981 cctcgatgtg tcgcggcgcg gtgagatagt gttccaggta cacgtccccg ttgccgaaag
     2041 cggacttggc ctcgttctgc gccgtgagga tggcctcctt caggaaggcc gggtcctgca
     2101 ccacgcgcat ccccttcccg ccgccgccgg ccttggcctt gacgatgacg ggaaagccga
     2161 tcttcttggc gatcttcagc agtttgggat tgttcgggtc cacgcagccg tcggaaccgg
     2221 ggatcaccgg cacgccgtaa ttctccatga tcttgcgggc ctcggccttg tcccccattt
     2281 tgcggatggc gtccttggac ggcccgatga acgtgatgtg gcaggtctcg cagacgtcgg
     2341 cgaaatgcgt gttctccgag aggaaaccgt agccgggatg gatggcgtcc gatccggtga
     2401 tctccgcggc ggaaatgatg gccgggatgt tcaaatagct ttcggcggcc gggccggggc
     2461 cgatgcagac cgactcgtcg gcccagcggg tgtggagggc gtcccggtcc acgtccgaat
     2521 ggacggccac ggtggcgatg ccgagttccc ggcaggcccg gatgatgcgg acggcgattt
     2581 cgccgcggtt tgcgatcagg atttttttaa acatagctcc ccttctctaa aaaatagccg
     2641 atgcgatgtc ctctgcggcg tctgagggcg atgcgttttt tgggacccgg cggcagacgg
     2701 cgaagcgggt ggtgccccgt cggggcacta ggcgggatca acctcaattt caaaaagttt
     2761 ttgcccgtat tccacgggtt ttccgttctg gaccagtatc ttggaaatct ttccgttcac
     2821 gtcggcgatg acgtctttct gcaccttcat ggcctccact tgggcgagcc gctggccggc
     2881 ggtcacctgg ccgccttcca ccaccagcgg cggccggtcc ttggagagtc cccgccaaaa
     2941 cgtgcccacg atgggcgaca ggatgtcttt ggtcttcgga gatttactct tcccgttgcc
     3001 gttggcggga accgcggagg ccgcggcggg cgcggccggc gccggcgggg cggcccggcg
     3061 cttgaaagcg atccggcggc cctccctctc ccacaccacc tcttccaact ccagctcctt
     3121 ggcgaaatcc accaaggcct tcacctgcag ggacggggtc gtctcgtcgg tcatcagagc
     3181 ctctccacgt attcgctggt gcgggtgtcc acccggatcg catcgccttc cttgacgaag
     3241 agcggcacct gaatctccat gccgttctcc agggtggcgg gcttcaccat gttggacacc
     3301 gaatccccct tgacgccggg aacggtgctg gtcaccttca gcgtgacgct ggcgggcaga
     3361 tccagcccca gaaattcctc gtcgatatag gccgcctgga cctccatgtt ttcggacagg
     3421 aaccgcgccg aatcgcccag ctgcgcgcgg gacacggtga tctggtcgta agtctccatg
     3481 tccatgaagg tgaaattgtc gccttcggca taaaggaact gtttcggccg acgggtgagg
     3541 ctgacctcct tgaaccgctc gcccgacttg aaggtgcgct cgatggtggc cccggtgcgc
     3601 atgttgcgga gtttcacgcg cagcatggcg ccgcccttgc cgggcttgtg atgctggaac
     3661 cacaccacct ggtaaaccgc cccgtcgtgc tcgaaaaccg taccgttgcc gatgtccgcc
     3721 gtgtcaatca tggatgtccc tcacattttc cccttcgatg gccgggtcag tatctccgaa
     3781 cccgtgtccg tcacccgcag agtgtcttcg atgcgcacgc cgaaccgccc cggaagataa
     3841 atcccgggct ctatggtcaa caccatcccg ggactcagaa cactgccact tttggggcgg
     3901 atccacggcg cctcatggat gtccaacccg acgccgtgac ccgtgctgtg gataaaataa
     3961 cgcccatatc cggcccggga aatgaccccg cgcgtggccg catcaatccg gccgccggtc
     4021 atccccggtt ttacgcgcgc tatcccctcg tgttgggccg cttggaccgt gcggtacacc
     4081 ctccgaaaca gagggaaaat tttaccgtaa taaagggtcc tcgtcaagtc ggcccgatac
     4141 ccccccacca tgcagccgat atccaatatc acgggctgat ttttgaccag ccgggagggg
     4201 ccggggaggt ggtgcggcag ggcggtgtgc ggccccatgg ccaccaccag atcgaaagcc
     4261 gccttgtccg ccccccggcg gtagaaaaac atctccaact ccgcggccaa gcctttttcc
     4321 gtcatccccg gtttaaggcg ttttttggcg aactcaatgc cgtccgccgt caattggcag
     4381 gcccgacgca cgcaggccag ctcctcctcc gccttgaccg ccctcagttc ggccgccgga
     4441 ttctcccccg gccgcaggcc ttccctcacc aaaatggcgc ccaatcggta catcaactgt
     4501 tccgaatcga acccgacccg cttcagaccg tgctttccca tcaaacttcg gagcgccttg
     4561 gacaaccgcc ggcccaccac cagccggcat ccctgcgtgc tgtcccgcac ctgcccggcc
     4621 aacagggcgg acgcgaagag ccaggtctcc ccctccgcgg taaaaagtcc gtagaacccg
     4681 tccgccatga atcccgtcag gtaatacagg tcggccatcc cgctgaaaaa ataggcgtcc
     4741 catttcttcc tctgcaggag ttccgacagc gcccggcggc gtcgacggaa aatctccgac
     4801 gtcggccggc cgaccgaaac gcgcttttcc atctgcaacg tcatggaatg tcttcctgcc
     4861 gaacggcgca atttttcatg ggagacggcg cggggagggg accggtcaaa tcccgatttc
     4921 ggtcagaacc ttatcgagcg ccgaccgggg aaaatccgag agggacttgg cctcgcccca
     4981 ccgcaccggc accacgaaac ggaaccggcc ggcataggcc tttttgtcgc gggccatggc
     5041 ggcgaggatc tttcttctag gaagcggccg gcgcacgcgg cagggcagcc cccaccgctt
     5101 caaaagggcc tccagccgag gcacgttctc tttggaaaaa atccccatcc ggccggccag
     5161 ccgcgcggcc gcgcacatcc ccacggcgat ggcttcgccg tgcgtgtatc ggccgtaacg
     5221 cgtcaccgcc tcgatggcgt gaccgaacgt gtgtcccaga ttgagcatct cccgctggcc
     5281 gcgcgtgtcg ttctcgtcct gcgccaccac ccgcgccttg atgcgcgccg aacgcgcgac
     5341 gatgtcctcg acgaggtcgg gccggcggcg gaacaccgcc aagtccacct tttccaaaag
     5401 cgccaggatg tccgggtcgg cgatgacgcc gtatttcacc acctcggcga tgccggtgcg
     5461 ccattcgcgg ggcggcaggg tcttcaacac ggatagatcc atccacacca gtttcggctg
     5521 ccagaacgcc cccaccaggt tcttgccttc cggcagatcc accccggttt tgccgccgat
     5581 ggaggaatcc accatgctca acagcgtggt cgggcactgg ataagcggca gtccgcgcag
     5641 atacgtggcg gccataaaac ccgccatgtc gcccaccacg ccgccgccca gcgccaccac
     5701 cgcgctctta cggtccagcc gcgccgtcac gccttcgcgg tagagccggc gcaccgtgtc
     5761 caggtttttg gccgcctcgc ccgacggcac cacggccaac gccggccgga acccggcctg
     5821 gcgcaatccg cgcaggagaa ccggcgcata acgccgcgcc acggggcggt cgctcaccac
     5881 caacgccgtg cggctcaagc cgaaaggcct caggttccgc cccaaatcac ggagcggcgc
     5941 gcccactttg atgaaatacc cgcgccccgc gaacagtttc accggaacgg tcttcacagg
     6001 caccgcctgc tttcgcttaa aaccatatta ttttccctcg ccttcaaacg aattttccat
     6061 cacccctttg cgagcgtttt gaaagaggcc tctttcggga gacggcgggc cttgctgttc
     6121 gaacccacat catggctgtt gatggggacg agttcaaggc cgcccgaaaa tgtccgactg
     6181 aatgctcgca ggggcaccct tccttttcgg ccgcttttct tcaggtgcgc gaagaaaagc
     6241 gacatttcaa aagaaacgca cctgttttta aaaattattt aaaaaaccaa actacaaaaa
     6301 catcaaacca ataatcttct ccgccacttc ttcggcgctc agaccgtcgg tatcgatgac
     6361 gtgatggcag cgggcatagg ccttctgccg cgaacgcaaa agctcctcca ccgccaacag
     6421 cgggtctttg cccttcaaca acggccgtgt gtcgatcccg tccctcagac gttccaacaa
     6481 agtctccggc cgggcgcgca gaaaaaaaac tttgccgttc cgttccaact cgtccatgtt
     6541 ctgcggccgc agcggaatgc cgccgcccgt ggcaatgacg catttgtcca aaagtcccag
     6601 cagctgcacc gtgcgggtct ccatgtcgcg gaaagcgggc tcgccctttt tggcgaagat
     6661 cgtggagatg gcgacgccgg cctctttttc gatcaggacg tcggtgtcgt agaacgtcca
     6721 cttcagccgg tcggccagga tcttgccgac cgccgacttg ccgctcccca tgaaacccgt
     6781 caacacgatg ttcatgcccg ccccctcaat gatcccggcg aaaaccggtc acagatgcga
     6841 gtgccgctgc cacacgtcgt cgaaaaccgc gcgcggcagt tcgtaacgga ccgtgccggg
     6901 tttcgccccc ggcccaacaa aaacgaatcg attcttttcc agcacccgga tggcggcagg
     6961 ccgctgccgg aatgtatcgg cgatcacgcg ggacacctgc ggacggccga acgcccaccc
     7021 caccagcccg ttcaccgcct ccgtggcgta cccccgtccc tgataatccg acaggatggc
     7081 gtaaccgaac tccacggtgc cgtccgccga cggcggcccc caaaaacccc cgcttcccac
     7141 ggcgacccgc cggccgaacg gttcgtcctt cagaaggaaa taccacttac accagccgtc
     7201 ggcatcgggt gaggtctcgt agaacctcag ggcgaaagac cgggtctgct cgtcgtttga
     7261 aaacagcggc cagcccgcgg ggatctccgc gtccaaaagg cgggcgaaag cggcccggtc
     7321 gccgatctcg gcctggacga tgtcccggtt gccggcgatc aattccagtc ggtgcgtttg
     7381 aatgcgcggt ccggtcggca tgcgaaacgg tatcttctaa aagttcctca ggtgatcgag
     7441 ataccgggcg tggttgtcct tcatctcgtg caccgaatcc ccgccgaact tttccttcac
     7501 ggcgttggcc agaacggtgg cggccaccgc ttcgcccacg atggcggccg acgccacggg
     7561 acacacgtcc gaccgcacga tctcggcgga catctcctct ttggtgagga tgttcacgga
     7621 cttaagcggc ttccgcaggg aggccagcgg cttcaccgcc gcctgcagca cgatgggttc
     7681 cccgttggtc atgccgccct ccacgccgcc ggcgttgttg gtcttccggt agaaaccgcg
     7741 agcggcgtcg tggaatatct cgtcgtgcag gtcggaaccc cgcttacggg tcatctcgaa
     7801 cccgtggttg aactccacgc ccttgtgcgc ctggatggag acgatggcct gcgccaggag
     7861 cgcgtccagt tttaagtccc actgggtatg gctccccaat cccggcggac aaccggtgac
     7921 gatcacggtg tatatgccgc cgagggtatc gccctcgtct ttggccttct tgatggcgtc
     7981 gacgatcttt tcctccgcgt gcttatcggg acagcgcacc ggcgactttt ccgccaactc
     8041 gaacgccgcc tccacggaca gtcccttgag atgggcgacg atgcccccga tctcggtgac
     8101 ataggaataa atcttgatac cgaactcctc caggagccgg cggcacaccg tgcccacggc
     8161 cgtgcgggcg gcggtctccc gcgcggaggc ccgctccaga atgtcccgcg tatcgcggcg
     8221 gtcgaacttc agcacaccgg ccaaatccgc gtgacccggc cgcggacgca ccaatcggaa
     8281 cttctccgac cggtccgcct cggacagaga catgatcttc tcccagttct cccagtcctt
     8341 gttctccacc accagggcga tgggcgagcc gagggtctct ccccagcgca cgcccgaggc
     8401 gatgcgcacc ttgtcctttt cgatgtattg caggcgctcc ccgcggccga aactgtactg
     8461 gcgccgcgaa agctgatggt tgatgaaatc ttccgtgatc ttcagtcccg ccggcacgcc
     8521 ttcgacgatg gccgtgaggc atttgccgtg ggattcccct gccgtcaagt agcgaatcac
     8581 tgtgcgttcc tctctttcaa ttcgttttcc actgtcttca tcatcacatc cacatccggt
     8641 ttctgatcga accaaatctc gaacgatcgg gcgccctggc gcaccagcat gccgagcccg
     8701 ccgaaagccc ggccgccttt tttcctggct tctctcacca attccgtttc gtggtggtaa
     8761 atcaaatcgt acaccgtcaa acccccgtgc agccactccg tggggaccgg cgattcgccc
     8821 ggtttcagcc ccaccggcgt ggcgttgatg aggagatgtg attgtccgat tctttccctc
     8881 aactcttccg cattggaaat ctgcgtcatc ggcaaaggaa acgccgggag ggtcgtgtcg
     8941 atttcctggc cctcccaccc tccggtcgcc tttttaaaac ttttcaacat ccccgtcgct
     9001 ttagcgacat cgcaatcata gacaaaaatg ctcttgggct tttcccgtcg ctcataaaaa
     9061 gagccgtaaa cggcgcggga ggcgccgccg gcccccagca atacgacatt ttttccgtcg
     9121 accgaaattt cgagttcttc caaatctcga gaaaaaccct cggcgtccgt gttgaagccc
     9181 cgccacccct cgttcgtgtt caagaccgtg ttggccgccc ccaaaaaaat ggccacgcgg
     9241 tccaattttg acccgcacag ggacgccgcc ttctctttat acggcaccgt cacgttgaaa
     9301 ccgaacaacg gcagttccct tgcctgtcgg aggaaatcgt cccactggtc cggttccaca
     9361 gggatggcct tatactccgc cctcagtccc agcgctttga tcgccgccgt atgcatcgcc
     9421 ggcgagaacg tgtgtccgag cggatatccc accacgccca gcttccgcgt gtcctctgac
     9481 acgttctcga aaatcttacg caggtccagc ttccccatgt ctttattcct tttaagactc
     9541 ctcagcccac tcgatggggc gtcgtcccaa cctctgttat ttaaagaggg acggccccga
     9601 aagccctgcg ggggaacggc ccccggcact ttcttacgct ccgccgacgg aaagtccttt
     9661 cacgcgaaac gtgggcgcgc ccacggcgcc gtaccacgtc aaatcgtccg ccaccgcgtc
     9721 caccccgttc agcatctcca aaagattccc ggccagggtc gctccccgca ccgcatggac
     9781 gcgccggcca ttgcgccaca ggaaaccgcc ggcccccacg gaaaaatctc cggacacccc
     9841 gtccgccatg tgcatcccga tgacgtcctc caccacgaac gcccgggccg tgtcccgcat
     9901 cagctccgcc gccggcacgc cgcccggttt cagataaaaa ttgctggccg acggcgacgg
     9961 cggcccgtcc acaccggacc ggttgccgtt gccggtgctg agacgatttt ctttccgggc
    10021 ggtgtagtgg tcgtacaaaa attcgcgcag aactcccccc gacaccgtcg ccgtctcccg
    10081 cgtggggcac ccttcgtcgt cgaagcgggc ggtggcgagg cctcccggat aacggccgtc
    10141 gtccaccaac gtcacggcgg aggaaccgac ccgtttcccc cttcgtccga cgaaaaacga
    10201 gcggccgtgc tgcaccgcgt ccgcgccgag cgcgtcggac aggagttcca aaaattccac
    10261 ccccgccttg ggatggaaca ccaccggcca tgttcccgaa aaaagcgcct tcgccccgaa
    10321 agcgcccacg gcctgtccac aggcgccggc cagcacccgc tccacgtcca gggattgcct
    10381 ggaacgggac gcgctccagt cccatcccac ctgcacgtct tttttccact cgcccaacac
    10441 ctcggcggag aaagaagcgt cgccgtaggg ttcccggacc gccacgccca tgctgctgaa
    10501 cagggcggac tccccgcgcg tctcgctgag acttaatttc aaaaccttct tgagccgacg
    10561 atccttcgcc aacgcccgtt tctcccaaaa agccagccgg tctcgaacct ccccgggaga
    10621 cccgcggaac aggtcgtcat ccccgggctc cgccgccggc tcgtcctctc ccggacgggg
    10681 cgcataaaac gttcgccgcg cgtccgccgg cgaaaatgcc gccgccgcgc gggcctggtc
    10741 ccacagccgc cgcgccgccc cggatgaaat ctcgcaggcg gacgcaaacc cttggcggcc
    10801 gcgatgaacg gcgcgcaccc ccatcccctg gctccaggag gacaccgtgt cgtccggccg
    10861 gccggccgac cacgcgaatt cgcgcgaccg ctcgcgttgg aaatagatct ccacctgcca
    10921 gcccgacgga acacggaggg attcgaaaat ccgttttccc tccccccaca aaccgtcttt
    10981 attgggacgg atgggcgggc tccttcttct caaaaagaac gcttttcccg gcgggatgct
    11041 cgggatcgat ccgcaacacc tgctcccagg ccttgcgtgc ctggtccttg cgccccagcg
    11101 ccgaccaaga cgcccccaac agcgaccagg catggacgtc gtttttgtcc ctcttcaaaa
    11161 tctcctggca gcgggcgatg ctggccttgt attgctgcgt ccaaaaacac cggtaagccg
    11221 cccgaaggtc cagataatcc gacggttttt ccagcgacat ctgatgcgcc atgttctgaa
    11281 accccgaata aaaaaacagg atggcgcaga ggaacaacag gatcccccac cggcggcgat
    11341 aaagtaaaac gtaactgtcg
//
LOCUS       DAOTFD010000063        11183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4113_length_11183_cov_4.545471, whole genome shotgun sequence.
ACCESSION   DAOTFD010000063 DAOTFD010000000
VERSION     DAOTFD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_11183_cov_4.545471"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="P5079_05410"
     CDS             <1..2340
                     /locus_tag="P5079_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY29461.1"
                     /translation="LFFTDLTRNPYFTQIVLLQAGVCLIVLLFVALAVRRGGFVLFPT
                     RLDVPLAVLFLWAVVTWGWSWWTHPPFRLPVWNEGKRVLLFLVWNGLAAYGISARLKS
                     EEWDSRFRRIFYVVGVIAGGYGVLQYMGRDFIWATALNPYNGRPVSTFGNPNFLSSYV
                     MMLLPLSLMDGFRAKTAGKRLFFGAVFLLFAAAVVCTMTRSSWIGSVAAVAVFLWFAR
                     RSLWSRRRWLGLVFGAAFLAIVFWPSSPLAGKSQRPVDRLRELVAGVTKEKSYGSWHQ
                     RLMIWSCAWDMVKERPVVGKGFGCFELFYPFYQGRYLSDRVFTVFRTHANNAHNVILE
                     IWSQAGTVGMGLFFWLCLLSAVFVWKRMPALSEEGGLELGAVFAAVVGMMVDNFFGNV
                     SVFFAVPAFLFWWLMGWLGRTVSPPETRTFLVRRPLVLAALLAATLVSAGGAVSAYRA
                     WRAEIAYFSGFKKSKQGDVRGAIEATERSHSYRRWEVNNNYELANAYARQARWAMDHK
                     LAQESRVFSEKALWAYAEALSANAGYDEIYFNRAAVLNQMERIPEAVLNYRMALLINP
                     LSVEAYRALGNLFLTKEGYFTGAEDLYQRALFYFPRDKEFWNNLGYVYTRLKRYEKAQ
                     DAYANALKLDLQFVLAAKNLQFTVKALGQTDHPLFAVPRLWGEVERAVAAKRWEEALR
                     AGRKLEALVPENTRVRLMLADVLAQSGGHAGAMDQYRLVLSWEPGNWEARRGLARVSV
                     LAGRKAEAVRMFRELLAERPGDRDVTDALRGLGESVLSK"
     gene            2431..3159
                     /locus_tag="P5079_05415"
     CDS             2431..3159
                     /locus_tag="P5079_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY29462.1"
                     /translation="MSGHSRWAGIKHKKGAIDAKRGKIFTKIVRELAVAAKTGGGNPE
                     NNPRLRKAMDDAKNANMPMDNVKKAIARGTGEIPGVTYEEVTYEGYGPGGAAVFLTGT
                     TDNRNRTTSEIRKIFSSHGGNLGESGCVAWMFQPKGQILVEKKAWPDEEKLMGMAIDA
                     GSEDFQSDEESYEIITSPADFEKVRGVLTAAQVPLAAAEQTLLPQNYVPLSGSAAEQM
                     LALMDDLENHDDVKEIYASCDIKE"
     gene            3248..3772
                     /gene="ruvC"
                     /locus_tag="P5079_05420"
     CDS             3248..3772
                     /gene="ruvC"
                     /locus_tag="P5079_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY29463.1"
                     /translation="MKVLGIDPGMATTGWGVVEKGRDQRCRAVRYGCILTPAATPMVE
                     RLQALARGLQRLIDDEKPDVAAVEELFFAKDSRTAANVGQARGVILYVLSQRGLPVHE
                     YNPRNVKMALTGYGGADKSQMQKMVQGLLSLKEMPKPDDAADALAIALCHLNTQRFAD
                     GPAGAVRPKTGERP"
     gene            3769..4389
                     /locus_tag="P5079_05425"
     CDS             3769..4389
                     /locus_tag="P5079_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRY29464.1"
                     /translation="MIASLRGTLLEKNAEGAVLEVAGVGYSVLLSETALAELPPLGEE
                     VLLSIAESVGMYGGGVTLYGFLRVEEKRLFNVLRENVPGTGAKKALELMDKALKSLPD
                     FRRAVLEKDTRALVSLFGFTRKTAEKMVAGLDGKMESLPISRGAGPAGMASSFDETIQ
                     ALVALGYKEPAARQAAQTARAAVGSEAAAKDLVREALRHLSGRGGL"
     gene            4410..5447
                     /gene="ruvB"
                     /locus_tag="P5079_05430"
     CDS             4410..5447
                     /gene="ruvB"
                     /locus_tag="P5079_05430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRY29465.1"
                     /translation="MSEKKPDAAALTPGALPDEETLDVTLRPQALDEFIGQNKLKENL
                     KVFIQAARKRNEPLDHCLFSAPPGLGKTTLAHIIAKELGVNLRQTSGPVLERVGDLAA
                     ILTSLGEGDVFFIDEIHRLNHAVEEALYPAMEDFALDIIIGQGPSAKTIKLSLPRFTL
                     VGATTRAGLLTGPLRERFGIMANLDFYEEGDLAAIVERSSRILSAKTEPAAAKEIARR
                     SRGTPRIANRLLRRVRDFADVRQDGRITPEITRGALEALEVDALGLDAVDRKILSALI
                     DKFGGGPVGIDTIAVAVSEERDTIEDVYEPYLIKAGFLLRTPRGRVVTPLAFSHLGKT
                     PPKSFKQSDLL"
     gene            5532..5747
                     /locus_tag="P5079_05435"
     CDS             5532..5747
                     /locus_tag="P5079_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRY29466.1"
                     /translation="MPNPGKTLVLWGGVIALLGGLLHLLPKIPVLGRLPGDFMIKREN
                     FTFYFPLATSLLISVLLSLLLRFLGKK"
     gene            5744..6910
                     /locus_tag="P5079_05440"
     CDS             5744..6910
                     /locus_tag="P5079_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRY29467.1"
                     /translation="MMFWFGPSFVRFLRNSVVLGTMLAVAFGSLRGGSRPPETIRIGI
                     MTETASVTMGPGAFLVREGADGKEHRLSWGDKFEVRVAGHGVLLGTKLLSGPVVVQPL
                     TPDAFPILNGRSYHGGLEFRRAPSGRLTVINELPLDDYVKGILIHETSPVWPFEALKV
                     QAVISRSYALNTRGRHGKEGYDLCDEIHCQVYGGRDSEREIPNRAVEETRGEVLTHEG
                     KIIKAVFHSCCGGSTENSENIWEGPALPYLKAVRCRWCRRSPRYKWVAEVRLDSAARK
                     LAAGGSDVGNIRMLRVLSRSRSGRAYRVRVAGSKGAAELSANAFRILLDGRVVRSTFW
                     TAVSQTRDSWRFDGKGWGHGVGLCQWGTKAMADAGRNYKTILHYYYRNVKLERR"
     gene            6997..8088
                     /gene="queA"
                     /locus_tag="P5079_05445"
     CDS             6997..8088
                     /gene="queA"
                     /locus_tag="P5079_05445"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRY29468.1"
                     /translation="MGFAEVPKERIAQSPLLRRDEARLMVLDRKTSTLAHRKFFEILE
                     FFRPGDVLVLNDVRIFPARLFGRKTTGGKVTMLLLRRCAGGPSGERWTALLTPSLKDG
                     TAVLLEEGVSARVEGKNETGEYVLSFSRDMVSYAERRGAVPLPPYIRRPAPVPEEVDR
                     RYYQTVYAQSGFDVSSSPSAPAAVGAVAAPTAGLHFTEDLLEKIRRRGVSVAALCLKV
                     GWGTFRPIGGGDYRQHRMLPEEYFVPEGTASAVNEARRAGSRVWAVGTTAVRALESAA
                     APGEPESGASARRHVVRAGGGETDLFIHPGYRFRVVDALITNFHIPGYTPLLMAAAFA
                     GADFLRQAYDEALRKDYRFYSYGDAMVIL"
     gene            8085..>11183
                     /locus_tag="P5079_05450"
     CDS             8085..>11183
                     /locus_tag="P5079_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29469.1"
                     /translation="MKRIPAFAGARFRRFVGVMGFFLIGGPAGPLAAAPKGKVPPPRM
                     PTTLEEMLSTVGISTSDVGLRADNLLQPYIPSVAAETLVRPMENVRKIQDWAEETLED
                     QASPSDFLNSAGRILEDRPAPTPLRPAGGFLLEKSEDVPEWLRVPLNDFLVVLSSATV
                     AVNEVNSVFSPEERAEWDGFVEELFSDSEKTSPEEPAGGETSVARRMRRVESLGRVDR
                     TEYLRAAQGIARALETLKKKLYAARSRWLEVSTLRFRTPAGWVTLGGVEDDMYTAADD
                     DVLLLDFGGDDVYRGRPASGDGRVSVLLDFSGNDSYAATVPHALGAGHWGVGLLCDLA
                     GEDVYQGARFSQGCGVFGAGVLVDEGGGDLYNALQVSQGAAFWGYGFLSDASGDDAYR
                     VDRMGQGFSGIGGLGLLADGRGNDQYLAGYSRADPREAGVFQTLSQGFSIGLRDYAGG
                     GVGALLDGAGHDFYKVDYFGQGASYWFSFGLLYDRDGRDTYVSRRYSQGAGVHSSAGL
                     LMDVRGDDTYLAWGGAQGLGWDFGVGELLDEQGNDIYSADWGAQGNGGKRGLGFFVDN
                     AGSDIYYVKKEHGQGFGDWDAESGAAGFGLFADISDGADRYPDPSREGGVWNGERWGV
                     GADLSSGALRGLSLRRDVPRFVDASAAQEKEERNVLEEKFARALASKNAEWKTAEMLD
                     LAGHWGLDTATPERARRWLLAASTGTMFPAVTASLDCRQTSGYMVQEEIYLRFGAVGL
                     DALSAHLAAMSEEAVASSTASARTKRFGVYYLGLTASTRPVAYLDGQLASSAWRDRAS
                     AVLALGRIHDARLRGLAAELAGFLGKKWMSGKEKVEFEEWVKRVGYRDRLKLIFNRQP
                     LPPAVFEKLMERSPVVKGSEWEKQWARFLWKERKFLAKEAEARSALFPSASLTGKIVA
                     LLSDGDPSVRQAAAVALGWMGDKTLESSARGDEAEGVVGDRLGQSLADPSAKVRMAAA
                     AAFAAMSPSSDRVLEAVFASSVPVARAGALEASALRKSPFLDDLLKAALKDADWSVRW
                     RAL"
BASE COUNT         1881 a         3200 c         3951 g         2151 t
ORIGIN      
        1 ctcttcttca ccgacctcac ccgcaatccc tatttcaccc agattgtcct cctgcaggcg
       61 ggggtctgcc ttatcgtttt gctgttcgtg gcgctggcgg tgcgccgggg gggcttcgtt
      121 ctttttccca cgcggctgga cgtcccgctc gcggtgctgt tcctctgggc cgtggtgacg
      181 tggggatggt cgtggtggac ccatccgccg ttccgcctgc cggtatggaa cgaggggaag
      241 cgggtgctcc tgttccttgt gtggaacggc ttggcggctt acgggatctc ggcccgtttg
      301 aaaagcgagg agtgggattc ccggttccgg cgcatctttt acgtggtcgg cgtcatcgcc
      361 ggagggtacg gcgttctgca atacatggga cgcgatttca tctgggccac cgccctcaat
      421 ccctacaacg gccgaccggt ctccacgttc ggcaacccca acttcctctc gtcctatgtg
      481 atgatgttgt tgccgttgag cctgatggac gggttccggg cgaagaccgc ggggaagagg
      541 ctttttttcg gggcggtgtt ccttcttttc gccgcggccg tcgtgtgcac catgacccgt
      601 tcgtcctgga tcgggtcggt ggcggcggtg gcggtcttcc tttggttcgc ccgccgatcg
      661 ctgtggtccc gccgccgttg gctggggctg gtcttcgggg cggccttcct cgccatcgtt
      721 ttctggccca gcagcccgtt ggccggaaag tcgcaacggc cggtggaccg tttgagggaa
      781 ctggtggcgg gcgtgacgaa ggagaagtcc tacggatcct ggcaccagcg gctcatgatc
      841 tggtcctgcg cctgggacat ggtgaaggag cgacccgtcg tcggcaaagg gttcgggtgt
      901 ttcgagctgt tctatccgtt ttaccagggc cggtacttgt ccgaccgggt cttcaccgtt
      961 tttcgaaccc acgccaacaa cgcccacaac gtgatcctgg agatatggtc ccaggccggc
     1021 acggtgggga tggggctttt tttctggctc tgtctgctga gcgccgtgtt cgtatggaaa
     1081 cggatgccgg ccttgtccga ggaggggggg ctggagttgg gggcggtgtt cgccgcggtc
     1141 gtcgggatga tggtggacaa ttttttcggg aacgtgtccg ttttcttcgc ggtgccggcg
     1201 ttcctgttct ggtggctgat ggggtggctg gggcggacgg ttagcccgcc ggaaacgagg
     1261 acgtttctcg tgcgccggcc gctggtgttg gcggccctgt tggcggcgac gctggtttcg
     1321 gcgggcgggg cggtgtcggc ctaccgcgcc tggcgggcgg agatcgccta tttcagcggg
     1381 ttcaaaaagt cgaagcaggg ggacgttcgc ggcgccatcg aggccaccga acggtcccac
     1441 tcgtaccgtc gatgggaagt gaacaacaac tatgagctgg ccaacgccta cgcccggcag
     1501 gcgcgctggg ccatggatca taaactggcg caggagtcgc gggtgttctc cgagaaggcc
     1561 ctgtgggcct acgcggaggc gttgtcggcc aacgccggat acgacgagat ctatttcaac
     1621 cgcgccgcgg tgttgaatca gatggagagg atcccggagg cggtgttgaa ttaccggatg
     1681 gcgctcctca tcaatcccct ttccgtggag gcctaccggg ccctggggaa cctttttttg
     1741 acgaaagagg gttatttcac cggggcggag gacctttatc aaagggcgct cttttatttc
     1801 cccagggaca aggagttttg gaacaacctc ggttacgtgt acacgcgcct gaaacggtac
     1861 gaaaaagcgc aggacgccta cgcgaacgcg ttgaagctgg acctgcagtt cgtgttggcc
     1921 gccaagaacc tccaattcac ggtgaaggcg ctgggacaga cggaccatcc gctgttcgcc
     1981 gtgccccgtc tctggggtga ggtcgaacgg gccgtggcgg cgaagcggtg ggaagaggcg
     2041 ttgagggccg gcaggaaact ggaggcgctg gtgccggaaa acacccgcgt gcgcctgatg
     2101 ctggcggacg tgttggccca gagcggcggg cacgccggcg cgatggacca atatcggctc
     2161 gtcctctcct gggaaccggg gaattgggag gcccgccggg gactggcccg ggtctccgtc
     2221 ctggccggcc gaaaagccga agccgtccgg atgttccggg aacttctggc cgaacggccg
     2281 ggagacaggg acgtgacgga cgctcttcga ggcctggggg aatccgtcct ctcgaaataa
     2341 gcgcgccgga aggtcgtgtt tacagtgaaa atcttatctt gtagaatctt ttcgcgcatt
     2401 tttaactcga aaaattagag gagaaaaagc atgtcgggtc attcacgttg ggcaggcatt
     2461 aaacacaaga agggcgctat cgacgcaaag cgcggcaaaa ttttcaccaa gatcgtccgg
     2521 gaactggcgg tggcggccaa gacgggcggc gggaacccgg agaacaaccc ccgtctgcgc
     2581 aaggccatgg acgacgccaa aaacgccaac atgccgatgg acaacgtcaa aaaagccatc
     2641 gcccgaggca ccggcgagat cccgggcgtg acgtacgaag aggtcaccta tgagggctac
     2701 ggccccggcg gcgcggccgt ttttctgacc ggcaccaccg acaaccgcaa ccgcaccacc
     2761 tccgaaatac ggaagatttt ttcctctcac ggcggcaacc tgggggagtc cgggtgcgtg
     2821 gcgtggatgt ttcagcccaa gggccagatc ctggtggaga agaaggcctg gccggacgaa
     2881 gagaagctga tggggatggc gatcgacgcc ggcagcgagg attttcagtc ggacgaggag
     2941 agctacgaga tcatcacctc tccggcggat tttgaaaaag tgcgcggtgt cttgacggcg
     3001 gcccaggtgc cgctggccgc ggcggaacag accctgttgc cgcaaaatta cgtgccgctg
     3061 agcgggtccg cggccgaaca gatgctggcc ctgatggacg atctggaaaa ccacgacgac
     3121 gtcaaggaga tctacgccag ctgtgacatc aaagaatagg gatgagggcg gggacgggcg
     3181 cgcggggaaa tgaaaacgag gtttgttttt tgacgccgcg cccgaaagct ccggccgcgt
     3241 tgttccgttg aaggttttgg ggatcgatcc cggcatggcc accaccgggt ggggcgtcgt
     3301 ggagaagggg cgggaccagc ggtgccgcgc ggtgcgttac ggctgcatcc tcactccggc
     3361 ggccacgccc atggtggaac gccttcaggc cctggcccgg ggactccagc gattgatcga
     3421 cgatgagaaa ccggacgtcg cggcggtgga ggagcttttt ttcgccaagg acagccgaac
     3481 ggcggccaac gtgggccagg cgcgcggcgt catcctgtat gtcctcagcc agagaggtct
     3541 cccggttcat gaatataacc ctcgcaacgt caaaatggcg ctgaccggat acggcggcgc
     3601 ggataaatcc caaatgcaga aaatggtgca gggtcttctt tcgttgaagg aaatgccgaa
     3661 acccgacgat gccgccgacg cgctggccat cgccctgtgt catctgaaca cccagcggtt
     3721 cgccgacggt ccggcggggg cggttcggcc gaagacgggg gagcgcccgt gatcgcgagc
     3781 ctgcggggaa cgcttctgga aaagaacgcc gagggcgcgg tgctggaggt ggccggcgtg
     3841 ggatactccg tcctgctgag cgaaacggcg ctggccgaac ttccgccttt gggagaggag
     3901 gtgctcctct ccatcgccga atcggtgggg atgtacggcg gcggcgtgac gctctatggg
     3961 ttcctccgcg tcgaggagaa aaggcttttt aatgtgctcc gggagaacgt gcccggcacg
     4021 ggcgccaaga aggccttgga gttgatggac aaggccttga aatctttgcc cgatttccgt
     4081 cgggcggtat tggaaaagga cacgcgggcg ctggtgtctc tgttcggttt cacccgcaag
     4141 acggccgaaa aaatggtcgc cgggctcgac ggcaagatgg aatccctgcc gatctcccgg
     4201 ggggccggcc cggcggggat ggcgtcgtcg ttcgacgaaa cgatccaggc cctggtggcg
     4261 ttgggctaca aggaaccggc ggcccggcag gccgcccaga cggcccgcgc cgccgtcggc
     4321 tcggaggccg ccgccaaaga tcttgtccgc gaggccctcc ggcacctgtc cgggcgcggc
     4381 ggattatgaa aggaaaacca gacgaatcga tgtccgaaaa aaaaccggat gccgcggccc
     4441 tgacccccgg cgcgttgccc gacgaggaaa cgctggacgt cacgctccgg ccgcaggcgc
     4501 tcgacgagtt catcggccag aacaagctga aggagaacct aaaggttttc attcaggcgg
     4561 cgcgcaaacg gaacgagcct ttggaccact gccttttttc cgcgccgccc ggcctcggca
     4621 agaccacgct ggcccatatc atcgccaaag agttgggggt gaacctccgg cagacgtcgg
     4681 gtccggtgct ggagcgggtg ggggacctgg ccgccatttt gaccagtctc ggcgaagggg
     4741 acgtgttctt catcgatgag atccaccggt tgaaccatgc ggtggaggag gctctgtatc
     4801 ccgcgatgga ggatttcgct ctggacatca tcatcggcca ggggccgtcg gctaagacca
     4861 tcaagttgtc tctgccgcgg ttcaccctgg tgggcgccac cacccgcgcc gggctcttga
     4921 ccgggccgct gcgggaacgg ttcggcatca tggccaactt ggatttttac gaggaagggg
     4981 acttggcggc catcgtggaa cgctccagcc gcatcctgtc ggccaagacc gaaccggcgg
     5041 cggcgaaaga gatcgcccgc cgttcgcgcg gcacgccgcg catcgccaac cggctcctgc
     5101 ggcgcgtgag ggatttcgcc gatgtgcgcc aggacggccg catcacgccg gaaattacgc
     5161 gcggggcctt ggaggccctg gaagtggatg cgctcgggct ggacgcggtg gatcggaaga
     5221 tcctttccgc cttgatcgat aaattcggcg gcggaccggt gggcatcgac accatcgccg
     5281 tggccgtcag cgaggagcgc gacaccatcg aggacgtgta tgaaccctat ctgatcaagg
     5341 cggggttcct cctccgcacg ccccgcggcc gcgtggtgac gcccctggcg ttttcccatc
     5401 tcggcaagac gccgcccaaa tcttttaaac agtccgatct gctgtgacga cccggcggtc
     5461 gggcgagtca tgagagagat ttgaccgaat cgcctcgccc ccgttcctct cagcagttcg
     5521 ccaaaaaaac tatgccgaat cctggaaaaa cgctcgtctt gtgggggggc gtcatcgcct
     5581 tgctcggcgg tctcctccat ctgcttccga aaatccccgt cctcggccgc ctgcccggcg
     5641 acttcatgat caagcgggag aacttcacgt tctatttccc gctcgcgaca tccctcctga
     5701 tcagcgttct cctgtccctc ctcctccggt tcttagggaa aaaatgatgt tttggtttgg
     5761 gccctccttc gtccgtttct tgcgaaactc cgttgtgttg gggacgatgc tggcggtggc
     5821 gttcgggagc ctccggggcg gttcccggcc gccggagacg atccgcatcg gcataatgac
     5881 cgaaaccgcc agcgtgacga tggggccggg ggcgtttttg gtgcgcgaag gggcggacgg
     5941 gaaggaacac cgtctttcct ggggcgataa attcgaggtg cgcgttgccg ggcacggcgt
     6001 ccttttgggg acgaaacttc tttccggccc cgtcgttgtg cagccgttga cccccgatgc
     6061 gttcccgatc ctcaacggcc ggtcctatca tggcgggttg gaattccgcc gggccccttc
     6121 gggacgattg acggtcatca acgagttgcc cctcgacgat tacgtgaagg gcatcttgat
     6181 ccatgagacg tccccggtgt ggccgttcga ggcgttgaaa gttcaggcgg tcatcagccg
     6241 gagttacgcc ctgaacacgc gcggccggca tggcaaagaa ggatacgatc tttgcgacga
     6301 gatccattgc caggtctatg gggggcggga ttcggaacgt gaaatcccca accgcgccgt
     6361 ggaggagacg cggggggaag tgctcaccca tgaggggaaa atcatcaagg cggtttttca
     6421 ttcgtgttgc ggcggatcga cggaaaattc ggagaacata tgggaagggc cggccctgcc
     6481 gtatttgaaa gcggtgcgct gccgctggtg ccggcgaagc ccgagatata agtgggtcgc
     6541 cgaggtccgc ttggacagcg ccgcgcggaa actggcggcc ggcgggtcgg acgtgggaaa
     6601 tatccggatg ctgcgggtgc tctcccggtc acgttccggc cgggcctacc gggtgcgcgt
     6661 ggcgggctca aagggggccg cggaactgag cgcgaacgcg ttccggatcc ttttggatgg
     6721 ccgcgtggtg cggagcactt tttggacggc ggtgtcccag accagggatt catggcggtt
     6781 cgacggcaag gggtgggggc acggcgtcgg cctttgtcag tgggggacca aggcgatggc
     6841 ggatgcgggg agaaattata agaccatatt gcactattat taccgcaacg tgaaactgga
     6901 gagacggtga gttcgctgaa aggtctcggg gagccggaga aacaggatcc gccgacggcc
     6961 ggggacgcgc ttcttgccgt ttccggcggc cgcacgctgg gttttgcgga ggtcccgaaa
     7021 gagcgcatcg cgcagtcccc gctcttgcgg agagacgagg cgcgcctgat ggtattggac
     7081 cggaaaacat cgacgttggc gcaccggaag ttctttgaga tcctggaatt tttccggccg
     7141 ggggacgtgt tggtcttgaa cgatgtgcgc atttttccgg cgcggctctt cgggcgcaag
     7201 accaccgggg gcaaggtgac gatgctcttg ttgagacgtt gtgcgggcgg accgtcgggg
     7261 gaacggtgga ccgcgctcct caccccctcg ttgaaagacg ggacggcggt ccttctggag
     7321 gaaggggttt ccgcccgggt ggaagggaaa aacgaaaccg gggagtatgt cctttctttt
     7381 tcccgggaca tggtttccta cgccgagcgt cgcggggcgg tgcctctgcc gccgtacatc
     7441 cgccggccgg cgcccgtgcc ggaggaggtg gaccgccggt attatcagac ggtgtatgcc
     7501 caatccggtt tcgatgtctc ctcgtcgccg tcggcccccg ccgcggtggg ggccgtggcc
     7561 gcgcccaccg ccgggctcca tttcaccgaa gaccttctcg aaaaaatccg ccggcggggc
     7621 gtgtcggtgg ccgcgttgtg tctgaaggtg ggctggggca cgttccggcc cattgggggc
     7681 ggcgattacc ggcaacatcg gatgttgccg gaggaatatt tcgttccgga aggaacggcc
     7741 tcggcggtga acgaggcccg ccgcgccggt tcccgggtgt gggccgtggg gaccaccgcg
     7801 gtccgagcgc tggaatccgc cgcggcgccg ggcgagccgg aaagcggggc gtcggcccgg
     7861 cgccatgtcg tgcgggccgg cgggggcgaa acggacctgt tcatccatcc gggataccgg
     7921 tttcgtgtcg tcgacgccct cattaccaat ttccatatcc ccggatacac gcccctcctg
     7981 atggccgcgg cgtttgccgg cgcggatttt ctgcggcagg cctacgacga ggcgctgcgc
     8041 aaggactacc gattttattc ttatggagat gcgatggtga ttttgtgaaa cggattccgg
     8101 ctttcgcggg ggcgcgcttt cgacggttcg tcggcgtcat gggctttttc ctgatcgggg
     8161 ggccggccgg cccgctcgcc gccgccccga aggggaaggt ccctccgccg aggatgccga
     8221 cgacgctgga agagatgttg tccaccgtgg ggatctccac gtcggacgtc ggccttcggg
     8281 cggacaatct gctccagccc tacatccctt cggtcgcggc ggagacgctc gtccgtccga
     8341 tggagaacgt gcggaaaatt caggattggg ccgaggagac attggaagat caggcctccc
     8401 cgtccgactt tttgaacagc gcgggccgga tcctcgaaga tcgtcccgcc ccgaccccgc
     8461 tccggccggc cgggggcttt cttttggaaa agtccgaaga cgtgccggag tggctgcgcg
     8521 tgccgctgaa cgattttttg gtcgttctgt cttccgcgac ggtggccgtg aacgaagtca
     8581 attccgtttt ttcgccggaa gaacgcgccg agtgggacgg tttcgtcgag gaacttttca
     8641 gcgattccga aaaaacgtct ccggaagagc cggcgggagg agaaacctcc gtggcccgcc
     8701 ggatgagacg ggtggagagt ttgggtcggg tcgatcgaac cgaatacctt cgagcggctc
     8761 aggggatcgc ccgggcgttg gaaacgttga agaaaaagtt gtatgcggcg cgaagccgct
     8821 ggctggaggt gtcgactctg cgtttccgca cgccggcggg gtgggtgacg ctcggcggcg
     8881 ttgaggacga catgtatacc gccgccgacg acgatgtcct ccttctcgat ttcggcgggg
     8941 acgacgtgta tcgcggccgg cccgcctccg gcgacggccg ggtgagcgtg ctgttggatt
     9001 tttccggcaa cgactcgtac gcggccacgg ttccccacgc gctgggcgcc ggacattggg
     9061 gcgtcgggct cttgtgcgac ctggccggag aggacgtcta ccagggggcg cgtttttctc
     9121 aaggctgcgg cgtgttcggc gccggcgtgt tggtcgacga gggcggcggc gacctgtaca
     9181 acgccctgca agtttcgcaa ggcgcggcgt tctggggata cggcttcttg tcggacgcct
     9241 cgggagacga cgcgtatcgc gtggaccgga tgggacaggg tttttccggc atcggcggtt
     9301 tgggactgct ggcggacggg cgaggcaacg atcaatatct ggccggatat tcgcgcgccg
     9361 acccccgcga ggcgggggtg ttccagaccc tctcgcaggg tttttccatc gggctccggg
     9421 actacgccgg cgggggggtg ggcgcgctgc tcgacggggc cggccacgat ttttataagg
     9481 tggattattt cggccagggg gccagttact ggttttcttt cggcctcctc tacgatcgcg
     9541 acggccgcga tacgtacgtc agccggcgat acagccaggg ggcgggggtg cattcttccg
     9601 ccgggttgtt gatggatgtg cggggcgacg atacctatct ggcctggggc ggcgcgcagg
     9661 ggttgggctg ggatttcggc gtgggcgagt tgctcgatga acagggcaac gacatctaca
     9721 gcgcggattg gggggcccag gggaacggcg gcaagagagg gctggggttt ttcgtcgaca
     9781 acgccggttc ggacatctac tatgtgaaaa aagaacacgg ccaggggttc ggcgattggg
     9841 acgcggaatc cggcgcggcc gggttcggcc tgttcgcgga tatttccgac ggcgcggacc
     9901 ggtatccgga cccgtcccgg gaaggggggg tgtggaacgg cgaacgatgg ggcgtggggg
     9961 cggacctttc cagcggagcg cttcggggtc tgagcctgcg gcgggacgtg ccgcggttcg
    10021 tcgacgcctc ggcggcccag gaaaaggagg agcgaaacgt cctggaagaa aagttcgccc
    10081 gtgcgttggc ttcgaagaat gcggaatgga agaccgccga gatgttggat ctggccggac
    10141 attggggact cgataccgcc accccggagc gggcgcgccg gtggcttttg gcggcctcca
    10201 cggggacgat gtttcccgcc gtcacggctt cgttggattg tcggcaaacc tcgggataca
    10261 tggtgcagga ggagatttac ctgcggttcg gcgccgtcgg tctcgatgcg ttgtcggccc
    10321 acttggcggc catgtccgag gaggcggtcg cctcctccac cgcgtcggcc cggacgaaac
    10381 gattcggcgt ctattatttg ggactgacgg cttccacccg gccggtcgct tacctggacg
    10441 ggcagctggc ttcttcggcg tggagggacc gcgcctcggc ggtgttggct ctcggccgaa
    10501 tccacgacgc gcgcctccgc ggacttgccg ccgagttggc ggggttcctg gggaaaaaat
    10561 ggatgagcgg caaggagaaa gtcgaattcg aggagtgggt gaaacgcgtg gggtatcggg
    10621 accggctgaa gttgattttt aaccggcagc cgctgcctcc ggcggttttt gagaaactca
    10681 tggaacggtc cccggttgtg aaagggtccg aatgggagaa gcaatgggcg cggttcctct
    10741 ggaaggaacg gaaatttttg gcgaaagagg cggaggcgcg ttccgcgctt tttccttccg
    10801 catcgttgac gggaaagatc gtcgcgctgc tgtcggacgg cgatccgtcg gttcgccagg
    10861 cggcggcggt ggcgttgggg tggatggggg acaagactct ggagtcctcg gcgcggggag
    10921 acgaggccga gggggttgtt ggcgaccgtt tgggtcaatc gctggcggat cccagcgcca
    10981 aggtgcgcat ggccgcggcg gcggctttcg ccgccatgtc gccctcgtct gaccgcgtgc
    11041 tggaagcggt tttcgcgtcg tccgttccgg tggcgcgcgc cggggcgttg gaggcgtcgg
    11101 ctctccgcaa gagcccgttt ctggacgacc ttttgaaggc cgcgctgaag gacgccgact
    11161 ggtctgtgcg ctggcgcgcc ctg
//
LOCUS       DAOTFD010000064        11016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4197_length_11016_cov_5.024815, whole genome shotgun sequence.
ACCESSION   DAOTFD010000064 DAOTFD010000000
VERSION     DAOTFD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_11016_cov_5.024815"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="P5079_05455"
     CDS             <1..820
                     /locus_tag="P5079_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29470.1"
                     /translation="LLFVGGVLAGSVRPAAAAPIAEEAAFDRLLVSWWKYQSLLTQPQ
                     RIGPGHLRMEATRDFLETPPLRKKRILEEVSSDWLRAFEATKASRPELLLAEITWARG
                     GWLWQVRRDEKTGEARMRLVDQWDDTRLPAMLDAVRKDKWFLFLGGQSVSGGKVGSSV
                     GFNMRLGTTLFRDKYDLSFLFNKTSTGPEPKTKATAYGVLGRRLFRLPNQWGYNLGGQ
                     ITAIKSSGFKAENEVGAVGGINYYLPKGTWDITLSLGDEGTRTLVIGYSIFFRS"
     gene            1270..2514
                     /locus_tag="P5079_05460"
     CDS             1270..2514
                     /locus_tag="P5079_05460"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY29471.1"
                     /translation="MSAGVLLGLLLGELAARHLCPETANGLRINKYMESERGKFCRYD
                     DTLGWAGKPGAEDRFEWADCVSRVRQNAYGFRGPAYPFARTARPRFVALGDSFVWGFG
                     VNEDELFTRRMEERSRSPVEVVNLGVSGYGTDQEYLLWQKTGRNWRPDHVLLFVTFVT
                     DLYDNVSPQRYGYPKPSAVLAAHNEVRLIPQDARQADLSRNAVRETTVPLARPHWQAA
                     LRRSDLLCLALRTLLRFDRRHFLETHGFVPAMCAGHDWETELFRDPPSEETRRLWLVL
                     AGLAKKLNEDVRRSGADLTVVLVPSVIQVYPKSWEKAVQNAPADFRLDPVLPARLLRE
                     VAEYSGARMVDLTPSLRAAARHRRVYCPLNGHWSSDGHRVVADALLNEFGLARPVSVF
                     FPRRKDSTFVSRLFFRRSPRPS"
     gene            2705..4096
                     /gene="glmU"
                     /locus_tag="P5079_05465"
     CDS             2705..4096
                     /gene="glmU"
                     /locus_tag="P5079_05465"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRY29472.1"
                     /translation="MTVPAVLVLAAGEGTRMKSSLPKVLHEAAGEPLLEHVFRAAAPL
                     RGPVGVVVGHGAERVRERYAGRCLFFLQKRRLGSGDAVKSAAAWLKRRGGDVVVLCGD
                     APLIRPGTLKRLTAVHRREKNAVTLLTARVSDPFGYGRIVRDAVGRPRAIVEHRDATP
                     EQRRIDEINSGMYCFRAKDLRAALKRLRPDNEKKEYYLTDAVAFLADRGRPVGALCLD
                     SEEEVLGVNRRSELAAADKILRRRTLEKLMAAGVTVVDPDSTYVDAGVRVGRDTVLYP
                     QTFLKGRTAVGRGGRIGPFSFLQDCRVGDDAELRAVFAYGSVVGPGARVGPFTHLRPG
                     TRIGAGARVGNFVETKNSRVGPKSKVSHLSYVGDAVLGADVNVGAGAITCNYDGFRKH
                     RTHIGSGAFVGSNANLVAPVRVGRGAIVGAGSTIVRDVPADALALERATEIVKKGWAR
                     RKRRELARIKAAE"
     gene            4127..5068
                     /locus_tag="P5079_05470"
     CDS             4127..5068
                     /locus_tag="P5079_05470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRY29473.1"
                     /translation="MSAGIKLFTGNAHRDLAKEIAKFLKVPLGEAMVGRFPDGEVDVK
                     IYENVRGADCFVIQPTSCPANDNLMELLLLVDALRRASAWRVTAVIPYYGYGRQDRKA
                     EPRVPISAKLVANLLSAAGTHRVLTMDLHAGQIQGFFDIPVDHLYANPVLIEYFKKKK
                     LINDKVVVVSPDAGGVERARAFAKRLKTGLAIIDKRRLSPHEAAVMHVVGEVRGRVAL
                     IIDDLVDTAGTLVKAAQALKDSGATRVLAAAAHGVLAGPAIERLNASPVEELVITNSI
                     PLDGKSTERLKVLSVAPILGEAIKRIHDERSISELFV"
     gene            5326..6072
                     /locus_tag="P5079_05475"
     CDS             5326..6072
                     /locus_tag="P5079_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRY29474.1"
                     /translation="MQTVELEAKLREITTRNALRLLRDSGEVPAVVYGGKKGPHPASV
                     NAKKMTEILKSQGTNVVFSLKLGKDSETVLLKDLQRDPISRKIVHVDFQRISLTEKIE
                     VNVPIHVKGEAPGVKLSGGILEHIQREVRVRCLPTAIPSAVEVDVSGLQINQGLKMKD
                     VKFPEGLEVLSDPELIVVNIVAPTEFKEETPAAAAAAVPGATEPEVIAKGKKPEEGAE
                     GAAPAAPGKEGAAKAPAAPAAKSPAGKEGK"
     gene            6091..6642
                     /gene="pth"
                     /locus_tag="P5079_05480"
     CDS             6091..6642
                     /gene="pth"
                     /locus_tag="P5079_05480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRY29475.1"
                     /translation="MQIVVGLGNFPAAYHRTPHNIGRLAVEALARRWRLEWVEKKDVL
                     DAAVREGRAVLVAPRSYMNTSGPAVLWARNRWKAPLEDVWVVCDDFTLPWGRLRIRRD
                     GRSGGHNGLESVIQSLGSTAFPRLRVGVGPVPEGMDPKDFVLGKQPRERLEKLAEQAA
                     DALEAAMDEGLESAMNRFNAARK"
     gene            6671..7648
                     /locus_tag="P5079_05485"
     CDS             6671..7648
                     /locus_tag="P5079_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY29476.1"
                     /translation="MKKMTLVVLMLAAAPVWSAPKAVHNPQKAQKKMRPQAPQSPLQK
                     AMNDLKSPDVALRRQAAEDLGTLRDAHATPALVVALSDEDAVVRSSASRALGLMRTPD
                     GVKELTRVLSTDPDAQVRQSAAVALGFIGDPQSVGPLTATLKDAEMGPRFAACQALAI
                     MKSPAAVPALKEALNDPSPQYKRTVIQALGDIGDVSAAPAVRPYIDDKDAQLQAAAIH
                     SLGKMKDTASASDLKKMLKKKTSSPELKMASAQALALQGNPAGRSAALEVLQDGTMDR
                     RVRLQAVRALAAMKDPSLAGPLKKLAEKEQDASVKNAVQSFLESLPPAR"
     gene            7659..8387
                     /locus_tag="P5079_05490"
     CDS             7659..8387
                     /locus_tag="P5079_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29477.1"
                     /translation="MRKQNMKRRFSLAALAAAGVFVFAALPLRAESGLVTWLVDTPTA
                     EIVDHYGYNLGFRLYSGGGVLTKGTFGVFPRLNVGFGLDAEKFIGADTVDLNPPTLNA
                     KFRLFDGKRYLPALALGYDGQGYFYDHSTDKYLQREKGLYLVGGEEVFTPGLTLTGGA
                     NIYDFSDDRIYAFAGVNYVYENFLGVVFELDNVHGKPRTNRMNAGVRCFVTPSVSVDV
                     SGRDLWAASRKAERIVVVNYIGSF"
     gene            8412..9449
                     /locus_tag="P5079_05495"
     CDS             8412..9449
                     /locus_tag="P5079_05495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRY29478.1"
                     /translation="MTTTETAVFKGFDFEKPVMDLEQQIQELQAIVKQNGVVGITDEI
                     RALQDRCEELKKEIYGGLTPWQRVQIARHPRRPYSLDYIEKIFGEFHELHGDRHFSED
                     KAIVGGVGFLDEMPVLVVGHQKGRTLQDSMARNFGMPHPEGYRKALRLMRLAARFSLP
                     IVTFIDTAGAYPGIGAEERGQATAIAENLREMAEIPVPIVCCIIGEGGSGGALAIGVG
                     NRLLMLENAWYSVISPEGCASILYRDAAKAPEAARALKLTATDLKELGIIDELVPEPL
                     GGAHRDADVTAANLKAALKKHFEDLLPLSPEKLLDQRYEKFRAIGHFQETQSKSLKPA
                     RRKTNKASASS"
     gene            9483..10535
                     /locus_tag="P5079_05500"
     CDS             9483..10535
                     /locus_tag="P5079_05500"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRY29479.1"
                     /translation="MSKKIVELLGKDAENLLKYKAKVPAANLHLPGPDFVDRVWALSD
                     RPAPVLRSLQAMFGTGRLAGTGYLSILPVDQGIEHSAGASFSPNPDYFDPENIVKLAI
                     EGGCNAVASTFGVLGSVARKYAHKIPFLAKINHNEFISYPNRYDQIMFGTVKEARDMG
                     CVAVGATIYFGSPESARQIIEVSQAFQMAHELGMATVLWCYLRNPAFKTAEKDYHTSA
                     DLSSQANHLGVTIQADIIKQKLPTNNGGYKALKFGKTHDKVYSDLSSDHPIDLTRYQV
                     LNCYMGRCGLINSGGESKGASDLAEAVTTAVINKRAGGTGLISGRKAFQRPMKDGAAL
                     LQSIQDVYLDKEVTIA"
     gene            10641..>11016
                     /locus_tag="P5079_05505"
     CDS             10641..>11016
                     /locus_tag="P5079_05505"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415175.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRY29480.1"
                     /translation="MTVLKVSELQKERAEFEPVLPALLRLGPASVSVRLGKATHSVAD
                     QETVRSLFPKTYGLPVVSFVKGKNPALRQKSVKVGVVLSGGQAPGGHNVLAGLLDGLK
                     KANPKNKLIGFLGGPSGILNNQW"
BASE COUNT         2055 a         3435 c         3652 g         1874 t
ORIGIN      
        1 cctgctgttc gtggggggcg ttctggccgg aagcgtccgt cccgccgcgg ccgcgccgat
       61 cgcggaggag gccgctttcg accgcctcct ggtctcttgg tggaaatatc agtccctcct
      121 cacgcagccc cagcggatcg ggccgggcca tctccgcatg gaggccacgc gggattttct
      181 ggagaccccg ccgctccgaa aaaaacgaat cttggaagag gtgtcgtcgg actggctccg
      241 cgcgttcgag gccacgaagg cgtcccggcc ggaacttttg ttggcggaga ttacctgggc
      301 gcgcggagga tggctctggc aggtccgccg ggacgaaaaa accggcgagg cgcggatgcg
      361 cctggtggat cagtgggacg acacccgtct tccggcgatg ctcgacgccg tccggaaaga
      421 caaatggttt ctgttcctcg gcggccagtc ggtgtccggc ggaaaagtcg gttcctccgt
      481 cgggttcaac atgcgactgg ggaccacctt gttccgcgac aaatacgacc tgtcgttcct
      541 tttcaacaag acctccacgg ggcccgaacc caaaaccaag gccaccgcgt acggcgtttt
      601 ggggcgacgt ctgttccgcc tgcccaacca atggggatac aacctgggcg gccagatcac
      661 cgccatcaaa tcgtccggat tcaaggcgga aaatgaggtt ggcgcggtgg gaggcatcaa
      721 ttactatctc cccaaaggga cgtgggacat caccttgagc ttgggagacg aggggacccg
      781 cacgctggtg atcgggtatt ccattttctt tcggtcgtga cagagaccga tccttgaact
      841 tttttgggtt cggacggacc gtttttcgac ggtcgaagtg agaagtgggg ggcctgcggc
      901 tcctcgaaca gatagcatcg gttcataaaa cgtgggaagc gtaaggccgc gcggcgggcc
      961 ttgcgctttt tttgtgggag ggacgggggg gaagaaagtt catcgggcgg ggttgtttta
     1021 ggaaatggcg ggggcgtgag gacagccaaa ccgtccggcc ttgggccggc gggcggacac
     1081 ccgaaacgag gcgaaggggt gtcccggggc ggaccttgcg tcttttcttt tgcgggacgg
     1141 aggaagggat ccatcgagaa aaagacggct ggagaacgaa gaatgggcca cgcagcgcca
     1201 ccgcgcaaaa aaaacgtttc cggaccgccg ccggcgggac ccggccggat ccttcgaccc
     1261 gcggcctttg tgtcggccgg cgtcctgctc gggcttcttc tcggcgagct cgccgcccgg
     1321 catctttgtc ctgaaaccgc caacggcctc cgcatcaata aatacatgga gtcggagcgg
     1381 gggaagtttt gccgatacga cgacaccttg ggctgggccg gaaagccggg agcggaagac
     1441 cgtttcgaat gggcggactg tgtctcccgg gtgaggcaga acgcttacgg tttccggggc
     1501 ccggcttacc cgttcgcccg gaccgcccgc ccgcgtttcg tggcgctcgg cgattccttc
     1561 gtgtgggggt tcggcgtcaa cgaggacgaa ctttttaccc gtcggatgga ggagcggagc
     1621 cgctcgccgg tggaggtcgt caacctgggc gtgtcgggat acggcacgga ccaggaatat
     1681 ctgctgtggc aaaagacggg ccggaactgg cggcccgacc atgtgctgtt gttcgtcacg
     1741 ttcgtcacgg acctgtatga caacgtcagc ccgcagcgtt acggataccc caaaccctcc
     1801 gcggtcctgg ccgcgcacaa cgaggtgcgg ctgatccccc aggacgcgcg gcaagccgat
     1861 ctttcccgga acgcggtccg ggaaacgacc gtgccgttgg cgcgtcccca ttggcaggcg
     1921 gcgttgaggc gcagcgatct cctttgcctg gcgctccgga cgctgcttcg gttcgaccgg
     1981 cggcattttc tggagaccca cggtttcgtc ccggccatgt gcgcgggcca cgactgggag
     2041 acggaacttt ttcgggatcc cccttcggaa gagacccgcc ggctgtggct ggtcctggcg
     2101 ggtttggcga agaaactcaa cgaggacgtg cggaggagcg gggctgacct cacggtggtc
     2161 ctggtcccgt ccgtgatcca ggtgtatccg aaatcgtggg aaaaggcggt gcaaaacgcc
     2221 ccggcggatt ttcggttgga tccggtcctt cccgcccggc tgttgcggga agtggccgaa
     2281 tacagtgggg cgaggatggt ggaccttacg ccctccctgc gcgcggccgc ccggcaccgg
     2341 cgggtgtact gccccctgaa cggccattgg agcagcgacg ggcaccgggt ggtggcggac
     2401 gcgttgctga acgaattcgg cctcgctcgg cccgtctcgg tgttttttcc gcggaggaag
     2461 gactccacgt tcgtttcccg gctgttcttt cgccgatcgc cgcgaccgtc ctgaacacaa
     2521 accgtttccg gttccacttt tcccattcgg cacgcagacg aggcaccgtc ttccccaacg
     2581 ctaaatggaa tattgtcagc cggcgcaata ggtggtagaa tagcgcgccg ggaaagagca
     2641 gtcgtacgca cgtccaaacc gtcgtcccga tgcaggaact ctcataaaaa ctggacacac
     2701 cctgatgaca gtcccagctg tccttgtttt ggccgcgggg gaaggcaccc gcatgaagtc
     2761 ttccctgccc aaagtgctgc acgaggcggc gggagaaccc ctgttggaac acgtttttcg
     2821 cgcggccgcg ccgttgcggg ggccggtggg cgtggtggtg ggccacgggg cggagcgggt
     2881 gcgggaacga tacgccggcc ggtgtttgtt ctttttgcag aagaggcggt tggggtccgg
     2941 cgatgccgtg aaatcggcgg ccgcctggtt gaaacgtcgc ggcggggacg tggtcgtcct
     3001 ttgcggcgac gcgccgctga tccggccggg aaccctgaag cgcctgaccg cggtccatcg
     3061 gcgggaaaag aacgccgtga ccctgctgac cgcgcgggtg tcggaccctt ttggatacgg
     3121 acggatcgtg cgggacgccg tcggccgccc ccgggccatc gtggaacacc gcgacgccac
     3181 gcccgaacag cgccggatcg acgagatcaa ttcgggcatg tactgtttcc gggcgaaaga
     3241 cctgcgggcg gccctgaaac ggctgcggcc ggacaacgaa aaaaaagagt attatctcac
     3301 cgatgccgtc gcttttttgg cggaccgggg ccggcccgtg ggggccttgt gcctggactc
     3361 ggaggaggaa gtgttgggtg tgaatcgtcg gagcgagctg gccgcggcgg acaagatttt
     3421 gcggcgccgc acgctggaaa aattgatggc ggccggggtg acggtggtgg atccggacag
     3481 cacctacgtg gacgccggcg tgcgcgtggg ccgggacacc gtcctctatc cgcagacgtt
     3541 tttgaaaggc cgcaccgcgg tgggccgcgg cgggcgcatc ggtcccttca gctttttgca
     3601 ggattgccgc gtgggcgacg acgcggaact gcgcgcggtg ttcgcctacg gcagcgtggt
     3661 ggggcccggc gcccgcgtgg ggccgttcac gcacctgcgg cccggcacgc gcatcggcgc
     3721 cggtgcgcgg gtggggaatt tcgttgagac caagaacagc cgcgtggggc ccaagagcaa
     3781 ggtgagtcac ctgtcctacg tgggcgacgc cgtcctcggc gccgacgtga acgtgggggc
     3841 cggggcgatc acctgcaact acgacgggtt ccgcaagcac cggacgcaca tcggatcggg
     3901 cgcttttgtg gggtccaacg ccaatttggt ggcgcccgtc cgcgtgggcc gcggcgccat
     3961 cgtgggggcg ggatccacca tcgttcgcga cgtccccgcc gacgcgttgg cgctggaacg
     4021 cgccaccgag atcgtcaaga agggctgggc ccggagaaaa cgccgggagt tggcccggat
     4081 taaagccgcc gaataggccg caaaaaacaa gggaggaacg cttcgcatgt ccgctggcat
     4141 aaaactgttc accggtaacg cgcaccgcga cctggccaag gagatcgcca aattcctgaa
     4201 ggtgcctctc ggcgaggcga tggtggggcg ttttcccgac ggcgaagtgg atgtgaaaat
     4261 ttatgagaac gtgcgcggcg cggactgttt cgtgatccag cccaccagct gccccgccaa
     4321 cgacaatttg atggaactcc tgctgctggt ggacgcgctc cgccgcgcct cggcctggcg
     4381 ggtcaccgcg gtgatcccct actacggcta cggccggcag gaccgcaagg ccgaaccgcg
     4441 ggtgcccatc tcggcgaaac tggtggccaa cctcctttcc gccgccggca cgcaccgcgt
     4501 gctcaccatg gacctgcacg ccggccagat ccaaggtttc ttcgacattc ccgtggacca
     4561 tctttacgcg aacccggtgt tgatcgaata tttcaagaag aagaaactca tcaacgacaa
     4621 ggtggtggtg gtctccccag acgccggcgg cgtggagcgg gcgcgggcct tcgccaaacg
     4681 attgaagacg ggcctggcca tcatcgacaa acgccgcctc tcgccgcacg aggcggcggt
     4741 gatgcacgtg gtgggcgagg tgcggggtcg ggtggcgctc atcatcgacg atctggtgga
     4801 caccgccggc acgctggtga aggccgccca ggcgctgaag gattccggcg ccacgcgggt
     4861 gctggccgcc gccgcgcacg gggtcctcgc cggaccggcc atcgaacggc tgaacgcctc
     4921 cccggtggag gagctggtca tcaccaattc catcccgctg gacggcaagt ccaccgagcg
     4981 gctgaaggtg ttgtccgtgg cccctatttt gggagaggcg atcaagcgga tccacgacga
     5041 acgctccatc agcgaactgt tcgtctgacg gtttttgcag taacgagagg acccgtctcc
     5101 gagaggggac ctcgggtcgc ccaggaacag ggacgacacc gagcgatcgg tccctttccg
     5161 gaaataggga cgaacccgta tccctaatct acggacaaga accgtgcctc gcggcacgag
     5221 cggcagatcc ctttccgcgc tttccgacag gcaagcccct cacctggcgg cgaggcaaac
     5281 tgggacgata cccagcggca tcgagggaaa aaaaggagaa gagtcatgca aaccgttgag
     5341 ttggaagcaa aactgcgcga gataacgacc cggaacgccc tgcgacttct gcgggacagc
     5401 ggcgaggtcc cggccgtggt gtacggcggc aaaaaggggc cccatccggc gagcgtcaac
     5461 gccaagaaga tgacggagat cctcaagtcg cagggcacga acgtggtctt cagcctgaag
     5521 ttgggcaagg attcggagac cgtccttctg aaggacctcc agcgcgatcc catcagccgc
     5581 aagatcgtgc atgtggattt ccagcgcatc tcgctgacgg agaagatcga ggtcaacgtg
     5641 ccgatccacg tcaaaggcga ggcgccgggc gtgaaactgt ccggaggcat tttggaacat
     5701 atccagcgcg aagtgcgcgt gcgttgtctg cccacggcca tcccgtcggc cgtggaggtg
     5761 gacgtgtccg gtctgcagat caaccagggc ctgaagatga aggacgtgaa gttccccgaa
     5821 ggcctggagg tcttgtccga tcccgaactg atcgtggtca acatcgtggc gcccaccgag
     5881 ttcaaggaag agacgcccgc ggcggcggcc gcggcggtgc ccggtgcgac cgaacccgag
     5941 gtcatcgcca aaggcaagaa acccgaggag ggcgcggaag gcgccgcccc ggcggccccc
     6001 ggcaaggaag gggcggcaaa agccccggcc gcgccggccg ccaaatcccc cgccggcaaa
     6061 gaggggaagt aaccgcttcc ctcagcgccc ttgcagatcg tcgtcgggct gggaaatttt
     6121 cccgccgcct atcaccgcac cccgcacaac atcggccgtt tggccgtgga ggcgttggcc
     6181 cgccgctggc gcctggagtg ggtggaaaag aaggacgtgc tcgatgcggc ggtgcgggaa
     6241 ggccgcgcgg tcctcgtggc gccccgttcc tatatgaaca ccagcggtcc ggcggtgctg
     6301 tgggcccgaa accgctggaa ggcgccgctg gaggatgtgt gggtggtctg tgacgatttc
     6361 acgttgccct gggggcggtt gcggatccgg cgcgacggcc gctccggcgg gcacaacgga
     6421 ttggagtcgg tgatccagtc cctggggtcg acggcgtttc cgcggctccg cgtcggcgtg
     6481 gggccggtgc cggaagggat ggacccgaaa gattttgttc tcggaaaaca gccccgcgaa
     6541 cggctggaga agctggccga acaggcggcg gacgcgttgg aagcggcgat ggacgagggg
     6601 ttggaatccg ccatgaaccg gttcaacgcg gctcggaagt aaatctcatc cggtggaggt
     6661 cgttgtttcc atgaaaaaga tgacactcgt tgttttgatg ctggcggcgg cgccggtttg
     6721 gtcggcgccg aaagcggtgc ataatcccca aaaagcccag aagaaaatgc ggccccaggc
     6781 gccgcagtcg ccgctgcaga aggcgatgaa cgatttgaaa tcccccgacg tggccttgcg
     6841 ccgccaggcg gcggaggatc tcggcacgct gcgggacgcc cacgccaccc cggcgctcgt
     6901 cgtggcgttg tcggacgagg acgcggtggt gcgttccagc gcgtcccggg ccctgggcct
     6961 gatgcgcacc ccggacggcg tcaaagaatt gacgcgcgtg ttgtccaccg atcccgacgc
     7021 ccaggtgcgg cagtcggcgg cggtggcgct ggggttcatc ggcgatcccc agtcggtggg
     7081 acccctcacc gccaccctca aggacgccga gatgggaccc cgtttcgcgg cctgtcaggc
     7141 gctggcgatc atgaagtccc cggcggcggt gccggcgttg aaggaggcgc tcaacgatcc
     7201 gtctccccaa tacaaacgga ccgtgatcca ggccctgggg gacatcggcg acgtttcggc
     7261 cgcccccgcc gtgcggccgt acatcgacga caaggacgcc cagctccagg cggcggccat
     7321 ccattctttg gggaagatga aggacaccgc gtccgcgtcg gacctgaaaa aaatgctgaa
     7381 gaagaagacg tcgtcccccg aactcaagat ggcttcggcc caggcgctgg cgttgcaggg
     7441 gaatccggcc ggccgttccg cggcgctgga ggttctgcaa gacgggacga tggaccggcg
     7501 cgtccgtctg caggccgtgc gggccctggc ggccatgaaa gacccgtccc tggccggacc
     7561 gttgaagaag ctcgcggaga aggaacagga cgcctccgtc aagaacgcgg tgcagtcttt
     7621 tctggaaagc cttccgccgg cccgttgagg gggatgacat gcgaaaacag aacatgaaac
     7681 gccgtttctc tttggccgcg ctggcggcgg ccggcgtctt cgttttcgcc gcgctgccgc
     7741 tccgcgcgga gtccggactc gtcacatggc tggtggacac ccccaccgcc gagatcgtgg
     7801 accattacgg ctacaacctg ggtttccggc tttattccgg cggcggggtg ctcaccaaag
     7861 gcaccttcgg ggtctttccc cggctcaacg tggggttcgg actggacgcg gaaaaattca
     7921 tcggggccga caccgtggac ctcaacccgc ccacgctcaa cgccaagttc cgcctcttcg
     7981 acggaaaacg gtatctgccg gcgctggcgc tgggctacga cggccagggc tatttctacg
     8041 atcactccac cgacaaatac ctgcagcggg agaaggggct ctacctggtg ggcggcgagg
     8101 aggtcttcac gcccggcctc acgctgaccg gcggcgccaa catctatgat ttttccgacg
     8161 acaggattta cgccttcgcc ggggtcaact acgtctacga gaacttcctg ggcgtggtct
     8221 tcgaactgga caacgtccac ggcaaacccc gcaccaaccg gatgaacgcc ggggtgcgct
     8281 gtttcgtcac ccccagcgtg tccgtggacg tctccggccg cgacctgtgg gcggcgagcc
     8341 gcaaggccga acgcatcgtg gtggtcaatt acatcgggag tttttgaccc gaatcccaca
     8401 ccctctcgat tatgacgacg acggaaacag cggttttcaa ggggttcgat ttcgagaagc
     8461 cggtgatgga cctggaacag cagatccagg aactgcaggc catcgtcaag cagaacggcg
     8521 tggtcggcat caccgacgag atcagggccc tgcaggaccg ctgcgaagag ttgaagaagg
     8581 agatctacgg cgggctcacg ccgtggcagc gcgtccagat cgcgcggcat ccgcgccggc
     8641 cgtactccct ggactacatc gagaagattt tcggggaatt ccacgaactg cacggcgacc
     8701 ggcatttttc agaggacaag gccatcgtcg gcggcgtggg gttcctggac gagatgcccg
     8761 tcctggtggt gggccatcaa aagggccgca ccctgcagga ttccatggcg cggaatttcg
     8821 gcatgccgca cccggaggga taccgcaagg ccctgcgtct catgcggctg gcggcccggt
     8881 tcagcctgcc catcgttact ttcatcgaca ccgccggggc ctatcccggc atcggggcgg
     8941 aggagcgcgg ccaggccacg gccatcgccg agaacctgag ggagatggcc gagatccccg
     9001 tgcccatcgt ctgctgcatc atcggcgagg gcggttccgg cggggcgctc gccatcggcg
     9061 tggggaaccg gctcctcatg ctggagaacg cctggtattc ggtcatctcg ccggaaggat
     9121 gcgcctccat cctctaccgc gacgcggcca aggcgccgga ggccgcccgg gccctgaaac
     9181 tcacggcgac ggacctgaag gaactgggca tcatcgacga actcgtgccg gaaccgctgg
     9241 gcggcgcgca ccgcgacgcg gacgtgaccg cggccaacct caaggccgcc ttgaaaaaac
     9301 actttgaaga tttgctgccg ctttctccgg aaaaattgct cgaccagcgc tatgagaagt
     9361 tccgcgccat cgggcatttc caggagacgc agtcgaagtc gttgaagccc gcccgtcgaa
     9421 agacgaacaa ggcttccgcg tcctcataaa acacgacatc agaacgggag aacatccaat
     9481 ccatgagcaa gaaaatcgtt gagcttctcg gcaaagacgc cgaaaatttg ttgaagtaca
     9541 aggccaaagt cccggccgcg aacctgcatc tgccggggcc ggatttcgtg gaccgcgtgt
     9601 gggcgctttc cgaccgtccc gcgccggtgc tccgcagctt gcaggccatg ttcggcacgg
     9661 gccggctggc cggcacgggg tacctctcca tcctccccgt ggaccagggc atcgaacatt
     9721 ccgccggggc gtcgttctcc cccaacccgg actatttcga cccggagaac attgtcaagc
     9781 tggccatcga aggcggctgc aatgccgtgg cttccacctt cggggtgctg gggtccgtgg
     9841 cgcgcaaata cgcccataag attcccttcc tggcgaagat caaccacaac gagttcatca
     9901 gctatcccaa ccgctacgac cagatcatgt tcggcaccgt caaagaggcg cgcgacatgg
     9961 gctgcgtcgc cgtgggggcc accatctatt tcggttcccc ggaatccgcc cggcagatca
    10021 tcgaggtgag ccaggccttc cagatggcac acgaactggg catggccacc gtgttgtggt
    10081 gctacctgcg caacccggcc ttcaagaccg cggagaagga ctatcacact tcggcggacc
    10141 tctccagcca ggccaaccat ctgggcgtca ccatccaggc cgacatcatc aagcagaagc
    10201 ttcccaccaa caacggcggc tacaaggccc tcaagttcgg caaaacgcac gacaaggtct
    10261 actccgacct ttccagcgat catcccatcg acctcacccg gtatcaggtg ttgaactgct
    10321 acatgggccg ctgcggactc atcaattcgg gcggcgagag caaaggcgcc tccgacctgg
    10381 ccgaggcggt caccaccgcc gtcatcaaca aacgcgcggg cggcacgggc ctcatctccg
    10441 gacggaaagc cttccagcgg cccatgaagg acggcgcggc ccttctgcag tccatccagg
    10501 acgtttacct ggacaaagaa gtcacaatcg cttaatcgac cttaggaacg gaatcggggg
    10561 ccggggatca gggacggaaa taattttttc cctgttaccc ggcccccggc accggctccc
    10621 cctttccagc ggagatcccc atgacggttt taaaagtttc cgaacttcaa aaagagcgcg
    10681 ccgaattcga gcccgtgttg cccgcgctgt tgcgcctcgg cccggcctcg gtgtcggtcc
    10741 ggttgggcaa ggccacccac agcgtggccg accaggagac cgtccgctcc ctttttccga
    10801 agacctacgg gttgccggtg gtgtcgttcg tcaaagggaa gaaccccgcc ctcagacaaa
    10861 aatccgtcaa agtcggcgtg gtcctttcgg gcggacaggc ccccggtgga cacaatgtgc
    10921 tcgccggcct tctcgacggc ctcaagaagg ccaaccccaa gaacaagctc atcggttttc
    10981 tcggcggtcc ttccggcatc ctcaacaacc aatgga
//
LOCUS       DAOTFD010000065        10890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4266_length_10890_cov_4.599631, whole genome shotgun sequence.
ACCESSION   DAOTFD010000065 DAOTFD010000000
VERSION     DAOTFD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4266_length_10890_cov_4.599631"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="P5079_05510"
     CDS             <1..685
                     /locus_tag="P5079_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29481.1"
                     /translation="VGGRTVEEDLRFHLGGADLIRVPVQGGGSSQFFADSNADLEFEG
                     EGNNAILRIAWRPGDGLHYIFKAGTGDFELRIPSGPATNEIVNGPPGRIWGAEVGWTL
                     MPDSPVTPALAVSLGYTRADYSMARLKSGSAEPVPVNQRFSLEEWQGAVCVSNRWGRV
                     EPYGGLRMMRHRSTLKDLDAAESVSGVRVGLAPYGGVKFEIFSREALVLEVSGLDETA
                     VTAGIAVGF"
     gene            996..1547
                     /gene="rplJ"
                     /locus_tag="P5079_05515"
     CDS             996..1547
                     /gene="rplJ"
                     /locus_tag="P5079_05515"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRY29482.1"
                     /translation="MPSVRKEREASVALLTKAFEDVQGLVVSEYTGMKTPELNELRSK
                     LRPLKGECRIVKNTLVQIALKNSGIEKFGEYFTGPSALVIQRGDAVTSLKVVIDFSKQ
                     HENLKVRAGYLNGQILSAAELKAVAALPPRIVLLSQLAGGLQGPLSRFHGVLSASLRN
                     LATALDQVSKKKAAAAPAGGGAA"
     gene            1629..2024
                     /gene="rplL"
                     /locus_tag="P5079_05520"
     CDS             1629..2024
                     /gene="rplL"
                     /locus_tag="P5079_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRY29483.1"
                     /translation="MAKCTIDDVLESIDEWSVLNLNKLVKGLEEKYGISAAPAMAMGA
                     VMPAAAGAAPGAASAEEKTEFSVVLVSAGDKKIQVIKAVRELTGLGLKEAKDLVEGAP
                     KPVKAGLTKEQAEEMKKKLAESGGTVEVK"
     gene            2106..5909
                     /gene="rpoB"
                     /locus_tag="P5079_05525"
     CDS             2106..5909
                     /gene="rpoB"
                     /locus_tag="P5079_05525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY29484.1"
                     /translation="MKKNSFARIDSVIELPPLLEMQLSSYEEFLQKDVPPEERKIQGL
                     QAAFLDVFPITSSDETLQLEFMHYSLGEPRYTVEEAISKDSSCSAQLRAWLRLVQKQP
                     GGKPKVLTEQEVYICDLPLMTKTATFVINGVERVVVSQLHRSPGVIFEEDEEKKISSY
                     GKKLYFGRLIPYRGAWVEFEFDLNNALYVRIDKKRKIAATTFLRAMGIETDADILSLF
                     YETETLALERVKPEEVIGRISARDVVDTSSGEVVLEVNREITREVFLRLQDRKVKTVD
                     VLKLDPQLNDVTIRNTLLKDTIRTRKEAIHAIYRVVRAQEFIVPEQAEAYIDNMLFKS
                     IRKYDLTKVGRFKINRKLMPFFEALAARKDFKFEIPNDRRRTLMREDIIATIQYLILM
                     NNEVAVFEHGKNVCKIEMDDIDHLGNRRIRSVGELLENQIRAAISQMARLVREKMNVQ
                     ENLQQMTPRTIVNASPVVAAVRRFFGSSQLSQFMDQTNPLAEMTHKRRLSALGPGGLH
                     RKRAGFEVRDVHHTHYGRLCPIETPEGPNIGLITSLACYARINQYGLIESPYRKVEKG
                     RVTDQIEYLTADKEDTYVIAQANAPLDKNGRFATDLVSCRYRADYPVKAPEDIDYMDI
                     SPVQVVSISAALIPFLEHDDANRALMGSNMQRQSVPLLFPEKPLVGTGVEHRVARDSG
                     ALEIARRSGVVLTVSGDQIVVWSEEGKPGVDIYTLRKYLRSNQDTCVNCVPLVKRGQR
                     VRKGDVLADGPGTQDGDLALGRNLLVAFMPWEGYNFEDAILLSERLVRNDIFTSIHIT
                     EFQVEARDTKMGAEEITRDIPNVGADSLVNLDDSGIIRVGADVVPGDILVGKVAPKGE
                     QPTTPEERLLKVIFGKKAEDVMDASLRVPPGVTGKILATKIFVRKEKLPRKEENRKVR
                     ELDAKLESMLEEIRSDRKDRFAVVEQAYEAGKLSKAEVNERKEWYKALSDRLEQEAKK
                     QIARDKENMKVGDELPVTVNKVVKVYIASKRKVQVGDKLAGRHGNKGVVAKILPPEDM
                     PYMPDGTPIDVVLSPLGVPSRMNVGQLLETMLGWAAHVLDAQMVTSVFDGATEEQIND
                     MVRQAKQKLREKGVPEEYLPTDDCRIQLYDGRTGEPFENKVTIGYMYMLKLAHLVEDK
                     IHARSTGPYSLITRQPLGGKAQFGGQRFGEMEVWAIEGYGAAHTLQEFLTVKSDDVQG
                     RTKMYEAIIRGEIVSEPGVPESFRVLVRELQSLGLNVELLRLEGGKSSTASKKAAAKE
                     AVAKE"
     gene            5919..10055
                     /gene="rpoC"
                     /locus_tag="P5079_05530"
     CDS             5919..10055
                     /gene="rpoC"
                     /locus_tag="P5079_05530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY29485.1"
                     /translation="MVTLFTERKRESSKKKSPVGMNFSNFDSMRLTIASPEQINSWSF
                     GEVRKPETINYRTFRPERDGLFCERIFGPVRDWECNCGKYKYIKYKGVICDRCGVEVT
                     ESKVRRERMGHIELAVPVAHVWFLRKSPSRIGTLLNLKLSDLERVVYYARYIVLEDYK
                     DADGKVRYKARQLFSDEECQKAREETRGRIRVGIGAGAIRELLEQVEVKKEADQVRKR
                     LKETDSEAERSRLIKRLRILDGFARSNTRPENMIMTVLPVIPPDLRPLVPLEGGRFAT
                     SDLNDLYRRIINRNNRLKHIESLRAPEVMVHNEKRLLQEAVDALLENGVHGKVVVGAG
                     NRPLKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKLHQCGLPKEMALELFK
                     PFIIRELMKKENVTLRAAKRMLERVKPEVWDILEQVTKKHPVMLNRAPTLHRLGIQAF
                     EPVLIEGKAIQLHPLTCTAFNADFDGDQMAVHVPLSLEAQLEARLLMLARNNILSPAS
                     GRPIAVPSQDMVLGCAYLTKQKLGVPGEGKVFSGPEEVISAYQQQQVDLHARIKVRGI
                     TPLNEDAGNDTPAKWKHYTTVGRIIFNEVIPPELGFVNTAVSKKELVVLVDRCYRELG
                     NHRTVVLLDAVKKLGYRYATMAGLSISISDMHIPKIKSELIKKARAEIKEIERQSKAG
                     VITDSERYNKIIDLWTHVTDQISDIMFDDMKKTELATYKKGDAKFNSVFMMADSGARG
                     SRQQIRQLAGMRGLMQKPQKKITGAVGEIIESPVISNFREGLTVLEYFISTHGGRKGL
                     ADTALKTADAGYLTRRLVDVAHDIVVMSDDCGTINGIRIGAIRSGEDIIEPLDERLVG
                     RFTLDNVVNPLTDELLIKAGEMVTAAKAKEIVAAGIDRMRIRSVLTCEAEFGVCAKCY
                     GLNLATGRLVEVGEAVGVIAAQSIGEPGTQLTLRTFHIGGTASRVIQKSQVAAQKGGT
                     VKFMNLRTIRNREGQLLCVTRNAVLSLREAGGQEKEHYDVPYGARLKVSDGKKAEPGE
                     ILAEWDPYSLPIISEYEGTAKLHDVIEGITMHEEKNRITGLIERVIIEHRAEQLHPQV
                     VVSKGGKKEASYPLPVDTNIVVQDGDGIQPGDVLAKIPQEVSKTKDITGGLPRVAELF
                     EARKPRNAAVISEIDGIVKLGMTQRGTMKVTVTNEETGLARDYSIPQGKHLVVYEGDR
                     VGVGESLTDGAINPHDILRVRGAKEVQEFLVNEIQEVYRLQGVGMNDKHIEIIVRQML
                     SNLLVTKSGSTDSLVGEIVSKGRFSRENREAAESDGEPAEAQPVLLGITKASLSSESF
                     ISAASFQETTRVLTDAASAGQVDGLRGLKENVIIGHLIPTGTGFYARTQAKVPPAAAE
                     "
     gene            10070..10456
                     /gene="rpsL"
                     /locus_tag="P5079_05535"
     CDS             10070..10456
                     /gene="rpsL"
                     /locus_tag="P5079_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006632197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY29486.1"
                     /translation="MPTINQLVTSGRESVTKKTKAPALEKSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLSTGVEVTAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIIRGPL
                     DATGVADRKQGRSKYGAKRPKQAEKK"
     gene            10466..>10890
                     /locus_tag="P5079_05540"
     CDS             10466..>10890
                     /locus_tag="P5079_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29487.1"
                     /translation="MPRKELKPRERRGMPDPDYKYQSVLAARFIGRLNFCGKKMTGER
                     IFYTALKLIQDKMKEEPLAVFTRAIENVRPLLEVRPRRVGGATYQVPQEVRPERGARP
                     LHHFGVLAHPFAAVRGTVRHRQLQALVAELDVLLNVVARA"
BASE COUNT         2354 a         3192 c         3453 g         1891 t
ORIGIN      
        1 cgtcggcgga cggaccgtcg aagaagactt gcggttccat ctcggcggcg ccgacctgat
       61 ccgggtcccg gtgcagggcg gcggttcgtc gcagttcttt gcagacagca acgccgacct
      121 ggagttcgag ggcgaaggca acaacgcgat actgcggatc gcctggcgac ccggcgacgg
      181 cctgcactac atcttcaagg ccggcacggg ggacttcgaa ctgcggatcc cgtcgggccc
      241 ggcgacgaac gagatcgtca acggcccgcc cggccggatc tggggcgcgg aagtgggctg
      301 gacgctgatg cccgattccc cggtgacgcc tgccctggcc gtgtccttgg gttacacgcg
      361 cgccgattat tcgatggcgc ggttgaaatc cggttcggcc gagccggtcc cggtgaacca
      421 gcggttctcc ctggaagagt ggcagggcgc cgtctgcgtc agcaaccgat ggggacgcgt
      481 ggagccgtac ggcggcctcc gcatgatgcg ccaccggagc acgttgaaag atttggatgc
      541 cgcggaaagc gtgagcggcg tgcgggtggg gcttgcgcct tacggcggcg tgaagtttga
      601 aattttttca agagaggccc tcgtgctgga ggtgtccgga ctggacgaga cggccgtgac
      661 ggcgggaatc gccgtcgggt tttgacagat tttcatagtg gctgttcgcg gcagagacag
      721 taggggtcgc acgtcgacat aagcgacagg caaataagag gtcagggttg aaggttgtaa
      781 gtccggatca caacacgacg ccttcagaga ttgacctgca acttacagct tcatcgctga
      841 ctttttatgg tcgccctacc gaggcgcgtt gaagtaacca ctcttcatcg tacttcctga
      901 ccataacgaa agtcttaaaa gagacggacc cggagttgat cgggccccgt cgcgggaagt
      961 ttgtttattt ttaaaaggga cgggaattcg gatctatgcc cagcgtaagg aaagaacgag
     1021 aagcatcggt cgcccttctg acgaaggcct ttgaggacgt ccagggcctg gtcgtgtcgg
     1081 aatacaccgg catgaaaacg ccggagctga acgagctgcg gagcaagctt cgtcccttga
     1141 agggcgaatg ccgcatcgtc aagaacaccc tggtgcagat cgccctgaag aacagcggca
     1201 tcgagaagtt cggcgagtat ttcacgggcc cttcggcgtt ggtaatccaa cgcggggacg
     1261 ccgtgacctc gctgaaagtg gtgatcgatt tttccaagca gcacgagaac ctcaaggtgc
     1321 gggccggcta tctcaacggc cagatcctga gcgcggcgga actgaaggcg gtggcggcgc
     1381 ttccgccccg gatcgttctt ctgtcgcagt tggcgggggg gctgcagggc ccgctctcgc
     1441 gtttccacgg cgttttgagc gcatcgttgc ggaacctggc gaccgcgctg gaccaggtgt
     1501 ccaagaaaaa agccgccgcg gcgccggccg ggggcggggc ggcctgacac gtttttggta
     1561 ggggagtggc agcaagctga aagctgggcg ggaaaagctt tggcgaaatt tcaaggaggt
     1621 tgacttaaat ggcaaagtgt acgatcgatg atgtgttgga gtccattgac gaatggagcg
     1681 tgttgaacct caataagctc gtcaagggat tggaagagaa atacggtatc tccgcggctc
     1741 cggccatggc gatgggcgcg gttatgcccg cggccgccgg cgcggctccc ggtgcggctt
     1801 ccgccgagga aaagaccgaa ttcagcgtcg tgctcgtttc cgcgggggac aagaagatcc
     1861 aggtcatcaa ggccgtccgc gaattgacgg gactgggcct gaaagaggcc aaggacctgg
     1921 tggagggcgc acccaaaccg gtgaaagccg gtctcaccaa ggaacaggcc gaggagatga
     1981 agaaaaagct cgccgagtca ggcggaacag tcgaggtcaa gtaaacggaa gacaagattg
     2041 ccgtttccgt ttgccgtcgt cgctgtttgt cggtaccgcc actttaactc gggagagctt
     2101 gtcgaatgaa aaagaattcg tttgcgcgca tagattcggt cattgagctt ccgcctcttt
     2161 tggagatgca gttgagctcc tacgaggagt ttttgcagaa ggatgttcca ccggaggaac
     2221 gcaagatcca gggccttcag gccgcgttcc tcgacgtgtt ccccatcacc agctccgacg
     2281 agacgttgca gctggagttc atgcattatt ccctggggga gccgcgttac accgtggaag
     2341 aggccatcag caaggacagc tcctgttcgg cccagttgcg cgcgtggttg cgcctggtgc
     2401 agaaacagcc cggcggcaaa cccaaggttc ttaccgagca ggaagtctac atctgcgacc
     2461 tgccgctgat gaccaaaacc gccacgttcg tcatcaacgg cgtggaacgc gtggtggtga
     2521 gccagctgca ccgttccccc ggcgtcatct tcgaggagga cgaggagaag aagatctcgt
     2581 cctacggtaa aaagctttac ttcggccgcc ttatccccta ccgcggtgcc tgggtggagt
     2641 tcgagttcga cctgaacaac gccctctacg tgcgcatcga caagaaaagg aagatcgccg
     2701 ccaccacgtt tttgcgggcg atgggcatcg agaccgatgc ggacatcctc tccctctttt
     2761 atgagaccga aaccctcgcg ttggagcgcg ttaagccgga agaagtgatc ggccgcatct
     2821 ccgcccgcga cgtggtggac accagctcgg gtgaagtggt gctggaagtc aaccgcgaga
     2881 tcacccgcga ggttttcctc cgcctccagg accgcaaggt caagacggtg gacgtcctga
     2941 aactggaccc gcagctcaac gatgtcacca tccgcaacac cctcctgaaa gacaccatcc
     3001 gcacgcgcaa ggaagccatc cacgccatct accgcgtggt gcgcgcgcag gagttcatcg
     3061 ttcccgaaca ggcggaagcc tacatcgaca acatgctttt caagtccatc cgaaaatacg
     3121 acctcaccaa agtgggccgg ttcaagatca accgcaaact catgccgttc ttcgaggcgc
     3181 tggccgcccg gaaagatttc aagttcgaaa tccccaacga ccgccgccgt accctgatgc
     3241 gggaagacat catcgccacc atccaatatc tcatcctgat gaacaacgag gtcgcggtat
     3301 tcgaacacgg gaagaacgtc tgcaagatcg agatggacga tatcgaccat ctcggcaacc
     3361 ggcgcatccg ctccgtgggg gaactcctgg agaaccagat ccgcgccgcc atctctcaga
     3421 tggcccgcct ggtgcgggag aaaatgaacg tgcaggagaa cctccagcag atgacgccgc
     3481 gcaccatcgt caacgcgtcg cccgtggtgg cggccgttcg ccggttcttc ggttcgagtc
     3541 agctttcgca gttcatggac cagaccaacc cgctggccga aatgacgcac aaacggcgtc
     3601 tctcggcgct ggggcccggc ggattgcacc gcaaacgcgc cgggttcgag gttcgcgacg
     3661 tgcatcacac gcactacggc cggctgtgtc ccatcgagac gccggaaggt ccgaacatcg
     3721 gattgatcac atccctggcg tgctacgcgc gcatcaacca gtatgggctg atcgagtccc
     3781 cttaccgcaa ggtggagaag gggcgcgtga ccgaccagat cgaatatctg accgccgaca
     3841 aagaggacac ctacgtcatc gcccaggcga acgccccgct cgacaagaac ggccgtttcg
     3901 ccacggacct cgtgagctgc cgctatcggg cggactatcc ggtgaaggcg cccgaggaca
     3961 tcgattacat ggacatctcg ccggtgcagg tggtctccat ctccgcggcg ctcatcccct
     4021 tcctggaaca tgacgacgcc aaccgcgctc tcatggggtc caacatgcaa cggcagtccg
     4081 tgcccctcct ctttccggag aagcctctgg tgggaacggg cgtggaacac cgcgtggcgc
     4141 gcgattcggg cgcgttggaa atcgcccgcc ggtccggcgt ggttctgacc gtttccggcg
     4201 accagatcgt ggtgtggagc gaagagggga aaccgggggt ggatatctat accctgcgga
     4261 aatacctgcg ttccaatcag gacacctgcg tcaactgcgt gccgctggtg aagcgtgggc
     4321 agcgcgtgcg caaaggcgac gtcttggccg atgggccggg cacgcaggac ggcgatctgg
     4381 ccctgggccg gaacttgctg gtggctttca tgccgtggga gggatacaac ttcgaagacg
     4441 ccatcctgct ctccgaacgg ctggtccgga acgacatctt tacctccatc cacatcaccg
     4501 aatttcaggt ggaggcgcgc gatacgaaga tgggcgccga agagatcacg cgggacatcc
     4561 ccaacgtggg ggcggactcc ctcgtcaacc tcgacgacag cggcatcatc cgggtggggg
     4621 cggacgtcgt ccccggcgac attctcgtcg ggaaggtggc gcccaaaggg gaacagccga
     4681 ccacgccgga ggaacggctt ctgaaggtaa tcttcggcaa gaaggccgaa gacgtgatgg
     4741 acgcttccct tcgcgtgccg ccgggcgtga cgggtaagat cctggccacg aagatcttcg
     4801 ttcgcaagga aaaacttccg cgcaaggaag agaaccggaa ggtgcgcgag ttggacgcga
     4861 aattggagtc catgctcgaa gaaatccgtt ccgaccggaa ggaccgtttc gccgtggtgg
     4921 aacaagccta cgaggccggc aagctgagca aggcggaggt caacgagagg aaagagtggt
     4981 acaaggcgtt gtccgaccgg cttgaacagg aagccaaaaa acagatcgcc cgcgacaagg
     5041 aaaacatgaa agtgggcgac gagcttcccg tcacggtcaa taaggtggtc aaagtttata
     5101 tcgcttccaa gcggaaggtg caggtcggcg acaaactggc cggccggcac gggaacaaag
     5161 gcgttgtggc gaagattctt ccgcccgaag acatgcccta catgcccgac ggaacgccga
     5221 tcgatgtggt gttgtctccg ctcggcgtcc cctcccggat gaacgtgggc cagttgctgg
     5281 agaccatgct cggctgggcc gcgcacgtgc tggacgccca gatggtgacg tcggttttcg
     5341 acggggccac cgaggaacag ataaacgaca tggtgcgcca ggccaagcag aaactgcggg
     5401 aaaaaggcgt ccccgaggag tatctgccga cggacgactg ccggatacag ctttacgacg
     5461 gtcgcacggg cgaaccgttc gagaacaagg tgaccatcgg ctacatgtac atgttgaagc
     5521 tggcgcatct ggtggaagac aagatccatg cccgttccac cggcccgtat tcgctcatca
     5581 cccgtcagcc gttgggcggc aaggcgcagt tcggcggaca gcggttcggt gagatggagg
     5641 tctgggccat cgagggatac ggcgcggccc atacgctgca ggaatttctg acggtgaaat
     5701 ccgacgatgt ccaggggcgc accaagatgt acgaggccat catccgggga gagatcgttt
     5761 ccgagccggg cgtgccggag tcgttccggg tgctcgtgcg ggaacttcag tctttggggt
     5821 tgaacgtgga actcctccgg ctcgaaggcg gaaaatcgtc gacagcttcc aagaaggccg
     5881 cggccaaaga agccgtggcg aaagagtagg agagggccat ggtgacgttg tttaccgaac
     5941 gcaaaaggga atcctccaaa aagaagtcgc cggtcggaat gaatttcagc aatttcgact
     6001 ccatgcggct caccatcgcc agcccggagc agatcaacag ctggagtttc ggcgaggtgc
     6061 gcaagcccga gaccatcaac taccgtacgt tccgtccgga gcgcgacggc cttttctgcg
     6121 agcggatatt cggccccgtg cgcgactggg aatgcaactg cggcaagtat aaatacatca
     6181 aatacaaggg cgtcatctgc gaccggtgcg gcgtggaagt gacggaatcg aaggttcgcc
     6241 gcgaacgcat ggggcacatc gagctcgccg tccccgtggc gcacgtgtgg ttcctccgca
     6301 agagcccctc ccgtatcggc accctgctca acttgaaact ttccgatctt gaacgcgtcg
     6361 tctattacgc ccggtacatc gtcctggagg attataagga cgcggacggc aaggtgcgtt
     6421 ataaagcccg ccagctcttt tccgacgaag aatgccaaaa ggcccgagaa gagacccgcg
     6481 gccgcatccg cgtcggcatc ggcgccgggg ccatccggga acttttggaa caggtggagg
     6541 tcaaaaaaga ggccgaccag gtgcgcaaac gcctgaagga gacggactcg gaggcggaac
     6601 gcagccggct catcaagaga ctgcggatcc tcgatggttt tgcccgcagc aacacgcgtc
     6661 cggaaaatat gatcatgacg gtgctgccgg tcatcccgcc ggacctccgt cccctggtgc
     6721 cgctggaagg cggccggttc gccacatcgg acctgaacga tctttatcgc cgcatcatca
     6781 accgcaacaa ccgtctcaag cacatcgagt ccctgcgtgc gccggaagtg atggtgcaca
     6841 acgagaaacg cctcctccag gaggcggtgg acgcgctgtt ggagaacggc gtgcacggca
     6901 aggtggtggt gggcgccggc aatcgtccgc tgaaatccct ttccgatatt ttgaaaggga
     6961 aacaggggcg cttccgtcag aaccttctcg gtaaacgcgt ggactattcg ggccgttctg
     7021 tgatcgtggt ggggccgaac ctgaaacttc accagtgcgg gctgccgaag gaaatggcct
     7081 tggaactttt caagcccttc atcatccggg aattgatgaa aaaggagaac gtgacccttc
     7141 gggccgccaa gcgcatgttg gaacgcgtga agcctgaggt gtgggacatt ttggaacagg
     7201 tgacgaagaa acatccggtg atgctgaacc gggcgcccac gctgcaccgc ctgggcatcc
     7261 aggccttcga accggtgctg atcgagggca aggccatcca gctccacccg ctcacctgca
     7321 cggctttcaa cgccgacttc gacggcgacc agatggcggt gcacgtgccg ctgtcgctgg
     7381 aagcgcagtt ggaagcgcgg ctactgatgc tggcgaggaa caacatcctc tcgcccgcct
     7441 ccggccggcc gatcgcggtg ccctcccagg acatggtctt ggggtgcgcc tatctcacca
     7501 aacaaaagct gggtgttccc ggcgaaggga aggttttttc cggccccgag gaagtcatct
     7561 cggcgtatca acagcaacag gtcgatctcc atgcccgcat caaggtgcgc ggcatcacgc
     7621 cgctcaacga ggacgcgggg aacgacacgc cggccaagtg gaaacattac accacggtgg
     7681 ggcggatcat cttcaacgaa gtgattcctc cggagctcgg gttcgtcaat acggccgtgt
     7741 ccaaaaagga attggtcgtt ctggtggacc ggtgttaccg cgaactgggc aatcatcgga
     7801 cggtcgtgct tctggatgcc gtcaaaaaac tcggctaccg ttacgccacg atggcgggac
     7861 tttccatctc catctccgac atgcacatcc ccaagataaa aagcgagctg atcaagaagg
     7921 cccgcgcgga gatcaaggag atcgagcgcc aatcgaaggc cggcgtgatc acggactccg
     7981 agcggtacaa caagatcatc gacttgtgga cgcacgtgac cgaccagatc tccgacatca
     8041 tgttcgacga catgaagaag acggaactgg ccacctataa aaagggcgac gcgaagttca
     8101 actcggtctt tatgatggcc gattcgggcg cccgcggaag ccgacagcag atccgccagc
     8161 tggccggcat gcgcggactg atgcagaagc cccaaaaaaa gatcaccggc gccgtcggtg
     8221 aaatcatcga gtctcccgtc atctccaact tccgcgaagg gctgacggtg ttggaatact
     8281 tcatctccac gcacggcgga cgtaaaggtc tggccgatac cgccctgaag acggccgatg
     8341 ccggctacct gacgcggcgg ctggtggacg tggcccacga catcgtggtg atgtcggacg
     8401 attgcggcac catcaacggc atccgcatcg gggccatccg ctccggcgag gacatcattg
     8461 agccgttgga cgagcgtctg gtcggccggt tcaccttgga caacgtggtc aacccgctga
     8521 ccgacgaact tctcatcaag gcgggcgaga tggtcaccgc cgccaaggcg aaggagatcg
     8581 tggccgccgg aatcgaccgc atgcgcatcc gttccgtcct cacctgcgag gccgagttcg
     8641 gggtttgcgc taaatgttac ggactgaatt tggcgacggg tcgtttggtg gaagtgggcg
     8701 aggcggtcgg cgtgatcgcg gcccagtcca tcggcgaacc cggcacgcag ctcacgctgc
     8761 ggaccttcca tatcggcgga accgccagcc gcgtgatcca aaagtctcag gtggccgcgc
     8821 aaaagggcgg caccgtgaag ttcatgaacc tccgcaccat ccgcaaccgg gaaggacaac
     8881 tgctttgcgt tacgcgaaac gcggtgcttt ccctccggga agcgggcggt caggagaagg
     8941 aacattacga tgttccctac ggcgcccggc tgaaggtgtc ggacgggaag aaggcggagc
     9001 ccggcgagat cctcgccgag tgggaccctt actccctccc catcatttcg gaatacgagg
     9061 gcacggccaa actgcacgac gtcatcgaag gcatcacgat gcacgaggaa aagaaccgca
     9121 tcacggggct gatcgagcgg gtcatcatcg agcatcgcgc ggagcagctt catccccagg
     9181 tggtggtgtc gaagggcggc aagaaagagg ccagctatcc tctgccggtg gacaccaaca
     9241 tcgtcgttca ggacggcgat gggattcagc ccggcgacgt cttggccaag ataccgcagg
     9301 aagtctcgaa aaccaaggac atcaccggcg gcttgccccg cgtggcggaa ctcttcgagg
     9361 cgcgaaaacc ccggaacgcg gcggtcatct ccgagatcga cggtatcgtt aagttgggga
     9421 tgacgcagcg cggcaccatg aaggtcaccg tgaccaacga agagaccggc ctggcccgcg
     9481 actacagcat cccgcaggga aaacacctcg tggtgtatga aggcgaccgc gtgggggtag
     9541 gcgaatcgct caccgacggc gccatcaatc cgcacgatat cctgcgcgtg cgcggcgcca
     9601 aagaggtgca ggagttcctc gtcaatgaaa ttcaggaagt gtaccgcctt cagggcgtgg
     9661 gcatgaacga caaacacata gagatcatcg tccgtcagat gctgtccaac ctgcttgtca
     9721 cgaaatccgg aagcacggat tccctggtgg gtgagatcgt cagcaagggg cggttctcgc
     9781 gcgaaaaccg ggaggcggcg gagagcgatg gggagcccgc cgaggcgcag ccggtgctct
     9841 tgggcatcac gaaggcctcg ctgtcgtcgg aatcgttcat ttccgcggcg tcgtttcagg
     9901 aaaccacgcg ggtgctcacc gacgcggctt ccgccggcca ggtggacggc ttgcgcggtc
     9961 tgaaagagaa cgtcatcatc gggcatctga tcccgacggg cacgggtttc tacgcgcgca
    10021 cccaggcgaa agtcccgcct gcggccgccg aatagcggag gtcttcgaaa tgccgacaat
    10081 caatcaactc gtcacttccg gccgggaatc ggtgacaaag aagacgaagg ccccggcgct
    10141 ggagaagtca ccgcagcgtc gcggcgtgtg tacccgcgtc tataccacca cgccgaagaa
    10201 gcccaactcg gccctgcgca aagtggcgcg tgtgaaactt tcgacggggg tggaggtcac
    10261 cgcctacatc ccgggggtgg ggcacaatct tcaggaacac tcgattgtgc tcgtccgcgg
    10321 cggccgcgtg aaggatctgc cgggagtgcg ctaccacatc atccgcgggc cgctcgacgc
    10381 caccggggtg gcggaccgca agcagggccg ttccaaatac ggcgccaaac ggcccaaaca
    10441 ggccgagaag aaataagggg ttgccatgcc acgaaaagaa ctgaaaccgc gcgaacgaag
    10501 agggatgccg gacccggact ataaatacca gtccgtcctg gcggcgcgct tcatcggccg
    10561 gttgaatttt tgcgggaaga aaatgacggg cgaacggatt ttttataccg ccctgaagct
    10621 cattcaggac aaaatgaaag aggagccatt ggccgtattc acccgcgcca tcgagaacgt
    10681 ccgtcctctg ctggaggtgc gtccccggcg tgtgggcggc gccacatacc aggtgccgca
    10741 agaagtccgg cccgagcgcg gcgcgcgccc gcttcaccac ttcggcgttc ttgcgcatcc
    10801 cttcgcggcc gtccgcggga ccgtgcggca tcgccagctt caggcgcttg tagccgaact
    10861 cgacgtgctg ctcaatgtcg ttgcccgtgc
//
LOCUS       DAOTFD010000066        10746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4332_length_10746_cov_4.371808, whole genome shotgun sequence.
ACCESSION   DAOTFD010000066 DAOTFD010000000
VERSION     DAOTFD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4332_length_10746_cov_4.371808"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /gene="pnp"
                     /locus_tag="P5079_05545"
     CDS             <1..1954
                     /gene="pnp"
                     /locus_tag="P5079_05545"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY29488.1"
                     /translation="SRLTDRTIRPLFPEHMRLDVQITSLALSSDGVNDTDIPAMLGAS
                     AALSVSDIPWGGPIGAVRVGRVEDRFVLNPTLEERAASTLELVVGGKKGAIMMVESGS
                     HELSEELLLEALELAQKEIDRLCDFQMEFVKKIGKPKFEAPLPAENADLVKRVEDAAR
                     PKYKEAVRIAEKTAREKFIDGIKEKVQADLAEAFPEQSGVIGGVLDRIEYEEARRLIL
                     DDKRRTDGRGFEDVRPITCETGVLPRTHGSALFTRGQTQALATVTLGSPSDQQIMDEL
                     QGEYKEHFLMHYNFPGFSTGEPKPERGPGRREIGHGALARRALLPLLPPQEEFAYTIR
                     IVSDIMESNGSSSMASVCGGSLALFDAGVPLQRPVAGIAMGLVKEGDRTAVLTDIMGM
                     EDHLGDMDFKVAGTDAGVTALQMDIKIEGISIAIMKQALEQAKRGRMHILGIMSQALS
                     APRPDLSPYAPRMVVVQIPVDKIGALIGPGGKNIRRIIEESGASVDVEDDGKVYITSV
                     DQTSMATAKGMVEELTAEVEVGKIYKGKVVRIMPRLGAFVEVMAGKDGLVHISQLDVQ
                     HVERVEDVVKVGDEIEVKCIEIDNQGRVNLSRKAVLKPGSELEGGRPQRFGGDRDRGD
                     RGDRGGGRGGFRGGRDRDRGPRRDRY"
     gene            1989..2474
                     /gene="accB"
                     /locus_tag="P5079_05550"
     CDS             1989..2474
                     /gene="accB"
                     /locus_tag="P5079_05550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRY29489.1"
                     /translation="MTDVKPNGKKKALEAQELQYYYDLMIAEDLLELEVREKDFYLRL
                     SRGRPASPAAAPAVVPSAPAAAAAASAADNVPPEQNQFHSIASPLAGVFYRSPSPQSG
                     PFVKEGDKVALNQVLCIVEAMKVMNEIRADRPSVIRKILVENGKPVAAGQNLFLVDPL
                     P"
     gene            2500..2700
                     /locus_tag="P5079_05555"
     CDS             2500..2700
                     /locus_tag="P5079_05555"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY29490.1"
                     /translation="MTNEIGRVAGDVWRYLERNGETSALRLRSALKVSQSQLFLALGW
                     LSREEKILLVTDEKGYRVSLKR"
     gene            2711..3487
                     /locus_tag="P5079_05560"
     CDS             2711..3487
                     /locus_tag="P5079_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY29491.1"
                     /translation="MTFLQSAVLGMVQGITEFLPVSSDGHLALFQLFLSVEPSLAFDV
                     AVHVGTLAAMVLFFGKDFVLLAKGLFAADPSIRDLERRRLGYIVLASVPTAAIGFSLK
                     SVVESSIVSLGAAGVGFLLTAGLLFGGERLARREGTPLAFTPWWHALVLGIAQGLAVW
                     PGLSRSGATIAVALALGWKWEEAGRFSFMMAVPAVAGATLLSARDILVLPKGPVLTGV
                     LVAFVAGLAALALLMRFLAARKLWPFAVYCAALGIISLIL"
     gene            3517..5775
                     /locus_tag="P5079_05565"
     CDS             3517..5775
                     /locus_tag="P5079_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRY29492.1"
                     /translation="MEAYFRSSSKAQLKVRRRQEAIGVALIVLGVLFFYSVLFPAGVW
                     GRHIKGGLGYLFGWAKSLFPLWMAYVGYRIAISKPWPNYAFRLTLWGSVFFFFCTLSS
                     LFGQAAYKLNYGGAVGLFGSNFLIRLLGVPGAWILTLIALGLTACGLLRLSPMEVFEK
                     VSERLRKDIQEWRQTRQQKVVRKPPVVKPRPLVISTDSKSSAAVAPAPEKTESPAPRP
                     VPASVPNIVPPTAEEPADVKKPVKVKAVKEEKSEPAPAPVPAAPPKPAYPGYQLPPAE
                     LLMDSKAQSVQQSESDLVAKAQLLEQTLANFGVTARATDIHPGPVITRFDLEPAPGVK
                     ISSIVNLADDIALAMKATRVRVLAPIPGKGAVGIEIPNPKSVTVTLKEIVESAPYQSS
                     KSLLTFVLGKTTSGEPTVADLAPMPHLLVAGATGSGKSVFIHSLIMSILFRAAPDKVK
                     FLLIDPKRLELPTYDGIPHLYDPRTKPEDAQVITQPKEAAKALARLVKVMEHRYEVFA
                     KANVRNIEGFNEKRLAAGQPPEFYIVVIIDELADLMLVATREVEDAIQRLAQMARAVG
                     IHLVLATQRPSVDVITGVIKANLPARVSFRVASQTDSRVILDTIGAESLVGSGDMLYL
                     PAGAPAPIRLQGAFVSEKEVESVVNFTRKQGRPQYEDIFAALAMAEAEQEDASTRTEL
                     CEALRLVLERRRVSQDLLKSQFGSSARATNILSLLEVHGFIHKPEGTNRWEIYFDKIE
                     DYLRAAESSNGE"
     gene            5783..6460
                     /locus_tag="P5079_05570"
     CDS             5783..6460
                     /locus_tag="P5079_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY29493.1"
                     /translation="MTASKLFLPSVLLASAVGLQAAAPKTDDVLARMKKAESDILTLS
                     FRFTQTADITLTGEKQKISGSAAFRKPDKFRIEHLSPQAQTVVSDGKTLWFYNPARNQ
                     VLVDSWANWTRSAGFPKGLLPYQTATDDLRERYDIVCESEVREKKRTGAVLRLTPKES
                     GPWTYTFRLWVDLDTGVPYRTELQTQGVNSVTDVTDVRVNPPLKESVFVFDVPKDAEI
                     LGGVPGQ"
     gene            6472..7257
                     /locus_tag="P5079_05575"
     CDS             6472..7257
                     /locus_tag="P5079_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRY29494.1"
                     /translation="MDIPSTSDSNRKETGLGEALRMRRLQRNLTIEFISQETRIQPRL
                     IKAMEEEDWKVFPARVYLEGFLRKYAALLGLDSADMMRRLAQSLGQPEKPLAIQPEVV
                     RKEDPVKETEDSSRRSGGILILFGILLVAGVYFHNSGMTKSVFSRARVSPSPPPEPVA
                     APVAAPLSGHKFVVRAKQSVWTRVIVDGRVKFEGILPPGTEKLWTGESEFRLVTGYPA
                     AVDVFADEEPLSVIEGGNAKLFRWPRDRSVPVSVNKSTGTLAP"
     gene            7254..8630
                     /gene="rimO"
                     /locus_tag="P5079_05580"
     CDS             7254..8630
                     /gene="rimO"
                     /locus_tag="P5079_05580"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRY29495.1"
                     /translation="MNVSARDPLAFVVLGCAKNQVEAETLASRLAQDGWKTTSDLSKA
                     STVVVHTCGFLESARDEALGAVARIRKTAPRAFLVLSGCFAQYLNGKRPQGVDALLGT
                     GQLDRLPALLSAGVQKRTVCSSPGGLHDASRPRPLRQGQLSAYVRVSEGCDRRCSFCI
                     IPQLRGAQKSRDPRDIEREVRGLLARGVREISLVSQETTAYGRDVSPSSDLPALLGRL
                     LRLPDLRWLRLLYTHPAGITGPLVRLLAEEEKLCGYLDMPLQHVSERMLRLMRRDYDQ
                     KSTMRLLERLRKEVPHLTLRTTFIVGFPGETERDFRQMRDVVAAGTFDHAGFFAYSPE
                     PRSPSARFPGRLPEEEMRERMDRILEAQTQVMRRKNESRIGQGVEALVETGPDGRWQA
                     RGLHQAPEVDGGIRLARWPMRSGFYNVRLTGTAGIDLTGRLAGDLRPRCQPVRSRSCV
                     EERSAVPA"
     gene            8627..9247
                     /gene="pgsA"
                     /locus_tag="P5079_05585"
     CDS             8627..9247
                     /gene="pgsA"
                     /locus_tag="P5079_05585"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRY29496.1"
                     /translation="MNLPNQLTLARLISLIPFMICAQAENVWAQTTALLIFIGAGVTD
                     LVDGYIARKHNLVTRLGVFMDPLADKLIITAAFITFANIPILHISAWMVILIVSREFV
                     ITGLRSLAAAHGQTMAADDGGKFKTTVQTTTVITTLVVLIIRTGLKDFSGIGGLEHPL
                     MSGWRASAMHVVEWIPYWLVFIATLFSLSTGISYLRKNKSLLQESV"
     gene            9247..9747
                     /locus_tag="P5079_05590"
     CDS             9247..9747
                     /locus_tag="P5079_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRY29497.1"
                     /translation="MILGRWMVLAVASVGFSSFLPYRLVPFAKWKGGGFIGSLAGWAL
                     LMALPNVGGVYWAATVLILASCVPVCHYAEKFLGNHDDPRIIYDEVAGMWVSALFLPK
                     DVWTLALAFVFFRLFDVLKGPWGRWASRAPGGWGVAGDDLVAGLLANVFVRLSLSLFP
                     QFLVPR"
     gene            complement(9876..>10746)
                     /locus_tag="P5079_05595"
     CDS             complement(9876..>10746)
                     /locus_tag="P5079_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29498.1"
                     /translation="YGILVWMGYLEIFRGLMFVVLFLLVVSWREMHGCRPGDDAPAAR
                     LLFSFSAAAGVLLLRKSLRLDATHYGFYLLAPALVLFAAFLFRFLPEHLRRRGSPSPC
                     HRFGMTAFLILAALFHWRLSLHGYRSKPHRLETPRGTFLLPKDEYATVRAALDYFQGR
                     DEGEFRLAVVPEGSFFNYFLDSVPVFYFNASLPDLVNTPEREDRFIRDFRDKDIKYVL
                     ITSRWASEYGAPIFGRDYLQRGFHHIRKRYRPVAQFGAPLFNPDEAFGALLLEKIPAS
                     PQNNPPPARPTTS"
BASE COUNT         1908 a         3403 c         3465 g         1970 t
ORIGIN      
        1 cagccggctg accgaccgca ccatccgtcc gctcttcccg gaacacatga ggctggacgt
       61 gcagatcacc agcctggcct tgtcgtccga cggggtgaac gatacagaca tcccggccat
      121 gctcggcgcc agcgcggcgt tgagcgtgtc cgacatcccc tggggcggcc ccatcggcgc
      181 cgtccgcgtg ggacgcgttg aagaccgttt cgtgctcaac cccaccctcg aagagcgtgc
      241 cgccagcacg ctggaactgg tggtgggcgg caaaaagggc gccatcatga tggtggagtc
      301 cggcagccat gagctcagcg aagaactcct cctggaggcc ctggagctgg cccagaagga
      361 gatcgaccgg ctgtgcgatt tccagatgga gttcgtcaaa aagatcggca agccgaagtt
      421 cgaagcgccc ctcccggcgg agaacgccga cctggtgaaa cgcgtggaag acgcggcccg
      481 gcccaaatac aaggaggccg tgcgcatcgc cgaaaagacc gcccgcgaaa agttcatcga
      541 cggtatcaag gaaaaagtcc aggccgacct ggccgaggcg ttcccggaac agtccggcgt
      601 catcggcggc gttctggacc gcatcgaata cgaggaggcc cggcgactga tcttggacga
      661 caagcgccgc accgacggcc gcgggttcga ggacgtccgg cccatcacct gcgagaccgg
      721 ggtcctgccg cgcacgcacg gctccgcgct cttcacgcgc ggccagacgc aggccctcgc
      781 caccgtcacg ctgggctctc ccagcgacca gcagatcatg gacgagcttc agggcgagta
      841 caaggagcat ttcctcatgc actataactt ccccgggttc agcaccgggg aacccaaacc
      901 ggaacgcgga ccgggccggc gcgagatcgg ccacggcgct ctggcgcggc gggccctcct
      961 gccgctcctg ccgccgcagg aagagttcgc ctacaccatc cggatcgtgt ccgacatcat
     1021 ggaatccaac gggtcctcct ccatggccag cgtctgcggc ggttctttgg cgctgttcga
     1081 cgccggcgtg cccctgcaac ggccggtggc gggcatcgcc atgggcctgg tgaaggaagg
     1141 ggaccgcacc gcggtcctca ccgacatcat gggcatggaa gaccacctgg gcgacatgga
     1201 cttcaaagtg gccggcaccg acgccggcgt caccgccctg cagatggaca tcaagatcga
     1261 aggcatctcc atcgccatca tgaaacaggc gttggaacag gccaagcggg gacgcatgca
     1321 catcctcggc atcatgagcc aggcgctctc cgcgccccgg ccggacctgt ccccgtacgc
     1381 tccgcgcatg gtcgtggtgc agatccccgt ggacaagatc ggcgccctca tcggacccgg
     1441 cgggaagaac atccgccgca tcatcgagga gtccggcgct tccgtggacg tcgaagacga
     1501 cggcaaggtc tacatcacca gcgtcgacca gacctccatg gcgaccgcca aaggcatggt
     1561 ggaagaactc accgccgagg tggaagtggg caagatatac aaaggcaagg tcgtccgcat
     1621 catgccgcgc ctgggcgctt ttgtcgaggt gatggcgggc aaggacgggc tggtgcatat
     1681 ttcccaactc gacgtgcagc acgtggagcg cgtggaagac gtggtcaaag tgggcgacga
     1741 gatcgaagtg aaatgcatcg agatcgacaa ccagggccgc gtgaacctct ctcgcaaagc
     1801 ggtgctcaag ccggggtccg aactggaagg cggccggccg cagcgtttcg gcggcgaccg
     1861 cgaccggggc gaccgcggtg accgcggagg cggccgaggg ggattccgcg gcggacgtga
     1921 ccgcgatcgg ggcccgcgcc gcgaccgtta ctgattcatt ccgtacggac gttgtttgag
     1981 gaggccggat gacggacgtt aaacccaacg gcaagaaaaa agcgttggaa gcgcaggaac
     2041 ttcagtacta ttacgacctc atgatcgccg aggacttgct ggagctggaa gtccgtgaaa
     2101 aggacttcta tctccggctg tcccgggggc ggcccgcttc tccggcggcc gccccggccg
     2161 tcgttccgtc cgctcccgcc gcggcggcag cggcgtcggc cgccgacaac gtccctcccg
     2221 aacagaacca attccattcc atcgcctcgc ccctggcggg ggttttctac cgttccccgt
     2281 cgcctcagtc cggccccttc gtgaaggagg gggacaaggt ggcgttgaac caggtgctct
     2341 gcatcgtgga ggccatgaag gtgatgaacg agatccgcgc cgatcggcct tccgttatcc
     2401 gaaaaatcct cgtggagaac gggaaaccgg tcgccgccgg acagaacttg ttcctggtgg
     2461 acccgctgcc ctgagccgtc gggggaagga cgttttggca tgacgaacga aattggccgg
     2521 gtggccggcg acgtgtggcg ttacctggaa cggaacgggg agacctccgc gctgcggctg
     2581 cgttccgcgc tgaaagtgtc gcagagccag ctcttcctgg ccctcggctg gctgtcgcga
     2641 gaagaaaaga tcctcctcgt gaccgacgaa aaaggttacc gggtttcgct gaagagatag
     2701 atcccctgcg atgacgttcc ttcagtcggc cgttctgggg atggttcagg ggatcacgga
     2761 attcctgccc gtttcatcgg acgggcacct ggcgctcttt caattgttct tgtccgtcga
     2821 gccttccctg gcgttcgacg tggcggtcca tgtgggcacg ctggcggcca tggtcctctt
     2881 tttcggcaag gacttcgtcc tgctggcaaa gggtctcttt gccgcggatc cgtccatccg
     2941 ggacttggaa cgtcgccggc tgggctacat cgttctggcg agcgtcccca cggccgccat
     3001 cggcttttct ttgaaatccg tggtggaatc ctccatcgtt tccctcggcg ccgcgggcgt
     3061 cggcttcctg ttgaccgccg gcctgctttt cggcggcgaa cgtctggccc ggcgggaggg
     3121 gacgcccctg gcgttcacgc cgtggtggca tgcgttggtc ttgggaattg cccagggact
     3181 ggcggtgtgg ccgggccttt cccggagcgg ggccaccatc gcggtggcgt tggcgctggg
     3241 ctggaaatgg gaggaggccg gccggttcag cttcatgatg gcggttccgg cggtggcggg
     3301 ggccacgctg ctttccgcga gggacatcct ggtccttccg aaggggccgg tgttgacggg
     3361 cgtgctggtg gccttcgtgg ccgggctggc ggcgctggcg ctgctgatgc ggtttttggc
     3421 ggcacgtaaa ctttggccgt tcgcggtcta ttgcgcggcc ctgggcatca tctccttgat
     3481 tctgtaaccc aagaaaggaa aaggacaaga gggactatgg aagcctattt cagatcgtca
     3541 tcgaaagcac aactcaaggt tcgccggcgg caggaagcca tcggcgtggc tctcatcgtt
     3601 ttgggcgtcc tgtttttcta ttccgttctc tttccggcgg gcgtgtgggg ccggcacatc
     3661 aagggggggc tcggatacct tttcgggtgg gccaagtccc tgttcccgtt gtggatggcg
     3721 tacgtgggtt accgcatcgc catttccaag ccgtggccca attacgcgtt ccggctcacg
     3781 ttgtgggggt ctgtcttctt tttcttctgc acgttgtcga gcctcttcgg gcaggcggcg
     3841 tacaagctca attacggcgg tgcggtgggg ctgttcgggt cgaacttcct gatccgcctc
     3901 ctcggcgtgc ccggcgcgtg gatactcacg ttgatcgccc tcgggttgac ggcctgcgga
     3961 ctgctgcgcc tgtcccccat ggaagtgttt gaaaaggttt cggagcgcct ccggaaagac
     4021 atccaggaat ggcggcagac gcgccagcag aaagtggtgc gcaagccgcc cgtggtgaaa
     4081 ccccggccgc tggtgatttc gacggacagc aagtcctcgg ccgcggtcgc gcccgcgccg
     4141 gaaaagacgg agagtcccgc gcctcgtccg gtgcccgcct cggttcccaa catcgtcccg
     4201 ccgaccgcgg aagagccggc cgacgtgaaa aaaccggtca aggtcaaagc cgtgaaggaa
     4261 gaaaaatccg aaccggcgcc ggcgcctgtc ccggccgccc cgcccaaacc ggcgtacccg
     4321 gggtaccagc tcccgcccgc ggaattgctg atggattcca aggcccagtc ggtgcagcag
     4381 tcggagagcg acctcgtggc caaggcccag ttgttggaac agacgctggc caatttcggc
     4441 gtcaccgccc gggccacgga catccatccc ggcccggtga tcacccggtt cgacctggaa
     4501 cccgcgccgg gcgtcaaaat cagttccatc gtcaatttgg ccgacgacat cgccctcgcc
     4561 atgaaggcca cgcgcgtgcg cgtgctcgcc cccatcccgg gcaagggcgc ggtcggcatc
     4621 gagatcccca accccaaatc cgtcaccgtc accctgaaag agatcgtcga gagcgcaccc
     4681 tatcaatcca gcaagtcgct cctgacgttc gtcctgggga agaccacctc cggggaaccc
     4741 accgtcgcgg acctggcgcc catgccgcat ttgctggtgg ccggcgccac ggggtcgggt
     4801 aaatccgtgt tcatccattc gctcatcatg tccatccttt tccgcgccgc gcccgataag
     4861 gtgaagttcc tgctcatcga ccccaaacgc ctggagctgc ccacctacga cggcatcccg
     4921 cacctctacg atccgcgcac caagcccgag gacgcgcagg tgatcaccca gccgaaagag
     4981 gccgccaagg ccctggcccg cctggtgaag gtgatggaac accgctacga agttttcgcc
     5041 aaagccaacg tgcgcaacat cgagggtttc aacgagaaac gtctggccgc cggccagccg
     5101 ccggagttct atatcgtggt gatcatcgac gagctggcgg acctgatgct ggtggctacc
     5161 cgcgaggtcg aagacgccat ccagcgcctg gcgcagatgg cgcgcgccgt gggcatccac
     5221 ctggttctgg ccacgcagcg gccgtcggtg gacgtgatca ccggcgtcat caaggccaac
     5281 ctgcccgccc gcgtgtcgtt ccgcgtggcg tcgcagaccg attcccgcgt cattttggac
     5341 accatcggcg ccgagtccct cgtcggttcc ggcgacatgc tctatctgcc ggccggcgcg
     5401 ccggcgccca tccgcctcca gggggccttc gtcagcgaga aggaagtgga gtcggtggtg
     5461 aacttcaccc gcaaacaggg ccggccccag tacgaggaca tcttcgccgc cctggccatg
     5521 gccgaggccg agcaggagga cgcctccacc cgcaccgaac tgtgcgaggc gctccggttg
     5581 gtgttggaac gccgtcgggt gtcgcaggac cttttgaaat cccaattcgg ttcttccgcg
     5641 cgcgccacca acatcctctc gctccttgaa gtgcacgggt tcatccataa accggaaggg
     5701 accaaccgtt gggagatcta cttcgacaag atcgaggact atcttcgggc ggcggagagc
     5761 tcgaacgggg agtagcctcc tcatgacggc gtcgaaattg ttcctgccct ccgtcctgct
     5821 ggcgtccgcc gtcggactgc aggcggccgc cccgaaaacc gacgatgtcc tggcccgcat
     5881 gaagaaggcc gagtccgaca tcctcaccct ttcgttccgg ttcacccaga ccgccgacat
     5941 cacgctcacg ggggaaaaac agaagatttc cggcagcgcc gcgttccgga agcccgataa
     6001 gttccgcatc gagcatcttt ctccgcaggc gcagaccgtt gtttccgacg ggaaaacgtt
     6061 gtggttttat aaccccgcgc ggaaccaggt tctggtggac agctgggcca actggacccg
     6121 gtccgccggg ttccccaagg gtctcctgcc gtatcagacc gccacggacg accttcggga
     6181 gagatacgac atcgtttgcg agtcggaggt gcgcgagaaa aaacggaccg gggcggtgtt
     6241 gaggttgacg cccaaggaat ccggcccgtg gacgtacacg ttccgccttt gggtggacct
     6301 cgacaccggc gttccgtatc gcaccgagct gcagacccag ggggtgaact ccgtgacgga
     6361 cgtgacggac gtccgggtca atcccccgtt aaaagaatcc gtttttgttt ttgatgtccc
     6421 gaaagacgcg gaaatcctgg gcggcgtgcc cggtcaatag gcgaaaaaat tatggatatc
     6481 ccatccacat ccgattccaa tcgaaaagag acgggcctcg gcgaggcgct gcggatgcgc
     6541 cgactgcaga ggaatttgac catcgaattc atcagccagg agacccgcat ccagcctcgc
     6601 ctcatcaagg ccatggagga ggaggactgg aaggtctttc cggcgcgggt ctatctggag
     6661 gggttcctgc gaaaatacgc cgccttgctc ggcctggatt cggcggacat gatgcgccga
     6721 ctcgctcaat ccctcggcca gccggagaaa cctctggcca ttcagccgga ggtggtgcgg
     6781 aaagaggatc cggtcaaaga gaccgaagac tcctcccgtc gatcgggggg gatattgatt
     6841 ttgttcggga ttttgctggt ggcgggcgtt tatttccaca attcgggcat gacgaaatcc
     6901 gtgttttcca gggcccgcgt ctcgccctcg ccgccgccgg aaccggtcgc ggcgcccgtg
     6961 gccgcgccgt tgagcggaca taagttcgtg gtgcgggcca agcagtccgt ctggacgcgc
     7021 gtgatcgtgg acggccgggt gaagttcgag ggcatcctgc cccccggcac cgaaaaactt
     7081 tggaccggag agagcgaatt ccgtctggtg acgggctatc cggcggcggt ggacgttttt
     7141 gccgatgagg agccgctctc cgtcatcgag ggcggcaacg ccaagctctt ccgctggccg
     7201 cgggaccggt ccgtcccggt ttcggtcaat aaatcgacgg gcaccctcgc gccgtgaacg
     7261 tctccgcccg ggatccgttg gcgttcgtgg tcctcggctg cgcgaaaaat caggtggagg
     7321 ccgagaccct cgcttcccgg ctggcgcaag acggctggaa gaccacctcc gatctttcca
     7381 aggcctccac ggtggtcgtc catacctgcg gatttttgga gagcgcccgg gacgaggccc
     7441 tgggggccgt cgcccgcatc cggaaaaccg ctccccgggc gttcctcgtg ttgtccggct
     7501 gtttcgccca atacctcaac ggcaagagac cccagggagt ggacgccttg ttgggcaccg
     7561 ggcagttgga ccgtctcccc gcactccttt ccgccggcgt tcagaaacgc acggtgtgtt
     7621 cgtctcccgg tggcctgcac gacgcctcgc ggccccggcc cctgcgtcag ggacagttgt
     7681 ccgcctatgt gcgggtttcc gaaggatgtg accggcgctg ttctttttgc atcattccgc
     7741 aactgcgggg cgcccagaaa agccgcgatc ctcgcgacat cgaacgggaa gtccgcggcc
     7801 tgctggcccg gggcgttcgg gagatttcac tcgtttcgca agagaccacg gcctatgggc
     7861 gcgatgtgag tccctcgtcc gatcttccgg ccctgctcgg gcggttgttg cgtctcccgg
     7921 acctccggtg gctgcggttg ctctacacgc acccggcggg catcaccggg ccgctggtcc
     7981 gtcttctggc ggaagaggaa aagctttgcg gatacctgga catgccgctg cagcatgttt
     8041 ccgagaggat gctgcgcctc atgcggaggg attacgacca gaagagcacg atgcggctgc
     8101 tggaacgttt gcggaaggag gttcctcact tgaccttgcg caccacgttc atcgtcggct
     8161 ttcccggaga gacggagagg gatttccggc agatgcggga tgtcgtggcg gcgggaacgt
     8221 tcgatcacgc gggattcttc gcctactcgc ccgagccgcg ttccccctcg gcgcgttttc
     8281 cgggccgtct gccggaggag gagatgaggg aacggatgga ccgtattttg gaggcacaaa
     8341 cccaggtgat gcgccgaaaa aacgaatccc gaatcggcca aggcgtggaa gcgctggttg
     8401 agacgggccc ggacggccgt tggcaggccc gcggcctcca tcaggccccg gaagtggacg
     8461 gcggaatacg tttggcccgg tggccgatgc ggtccgggtt ctacaacgtc cgcctcaccg
     8521 gaaccgcggg gatcgatttg accggccgtc tcgccgggga ccttcgtccc cggtgtcagc
     8581 ccgtgcgttc ccgttcctgc gtcgaagagc ggtcggcggt gcccgcatga atctcccaaa
     8641 ccaattgacg ctggcccggc tcatctccct catcccgttc atgatctgcg cccaggcgga
     8701 gaacgtctgg gcccagacca ccgctctgct catcttcatc ggcgccgggg tcaccgacct
     8761 ggtggacggc tatatcgccc gcaaacataa tctggtcacc cggctgggcg tctttatgga
     8821 tcctttggcg gacaaactca tcatcaccgc cgctttcatc accttcgcca acattcccat
     8881 cctgcacatc tcggcctgga tggtcatcct catcgtcagc cgggaattcg tcatcacggg
     8941 cctgcgttcg ctggcggccg cccatggcca gaccatggcc gccgacgacg gggggaagtt
     9001 caagacgacg gtgcagacca ccacggtcat caccacgctg gtggtcctca tcatccgcac
     9061 gggcctcaag gatttttcgg gcatcggcgg actcgaacat cccttgatgt cgggctggcg
     9121 ggcgagcgcc atgcatgtcg tggaatggat cccttactgg ctggtgttca tcgccaccct
     9181 cttttccctc agcaccggca tcagctacct gcgaaaaaac aagtccctcc tgcaagaaag
     9241 cgtttgatga ttctcggccg ctggatggtt ctggccgtgg ccagcgtcgg gttctccagt
     9301 ttcctgccct accgcctcgt ccccttcgcc aaatggaaag gcggagggtt catcggcagt
     9361 ctcgccggct gggcgttgtt gatggctctt ccaaatgtcg ggggggtgta ttgggcggcg
     9421 acggtgttga tcctggcgag ttgcgtgccc gtgtgtcatt acgccgaaaa attcctcggc
     9481 aaccacgacg acccccgcat catatacgac gaggtggccg gcatgtgggt ttcggcgctg
     9541 ttcctgccga aggatgtctg gaccttggcg ctggcgttcg ttttcttccg gctcttcgac
     9601 gttctcaagg ggccgtgggg ccggtgggct tcccgcgcgc cgggcggctg gggagtggcc
     9661 ggcgacgacc tcgtggccgg gcttcttgcc aacgtcttcg tccgcctttc cctttccctc
     9721 ttcccgcaat tcctggtccc ccgataatcc gcgatcctcg tgcgccggag gaacgttttt
     9781 gccggcgcgg tgactttttt ccccgcttcg ttccctcgaa aaacaacaca cgtgtcttgt
     9841 tcgagcgaaa tcgagaacga aacggttgcc ggccgtcagg aggtcgtcgg tcgggcgggc
     9901 ggcgggttgt tttgagggga ggccggtatt ttctcgagca ggagggcgcc gaaggcctcg
     9961 tcggggttga agagcggggc accgaactgg gccaccggcc ggtaacgttt tcggatgtgg
    10021 tggaacccgc gctggagata gtcccggccg aagatgggag cgccgtactc gctcgcccag
    10081 cgggacgtga tgaggacgta tttgatgtcc ttgtcccgga agtcgcggat gaaccggtct
    10141 tcccgttcgg gcgtgttcac caggtccggc aatgaagcgt tgaagtagaa aacagggacg
    10201 gagtcgagaa agtagttgaa gaaagaccct tccggcacga ccgccaggcg gaactcccct
    10261 tcgtctcgcc cttgaaaata atccagcgcc gcccggaccg tcgcgtattc gtctttcggc
    10321 agcaggaacg tgcctctcgg cgtctcgagc cggtggggct tggaacggta cccgtgcagg
    10381 gagagccgcc agtggaagag ggcggccaag atcaggaagg ccgtcattcc gaatcggtgg
    10441 catggagagg gcgagccccg tcggcggaga tgttcgggaa ggaaacggaa gaggaacgcg
    10501 gcgaagagga ccagcgccgg cgccagtagg tagaaaccgt agtgggtggc gtccaggcgc
    10561 aaagattttc gaagcaggag gacgcccgcc gccgccgaga aactgaagag aagccgggcg
    10621 gccggggcgt cgtcccccgg tcgacagcca tgcatttccc gccaggaaac caccagaaga
    10681 aacagaacca cgaacatgag tccgcgaaaa atctccaaat accccatcca cacgaggatc
    10741 ccgtag
//
LOCUS       DAOTFD010000067        10487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4462_length_10487_cov_4.320073, whole genome shotgun sequence.
ACCESSION   DAOTFD010000067 DAOTFD010000000
VERSION     DAOTFD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_10487_cov_4.320073"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="P5079_05600"
     CDS             complement(<1..1266)
                     /locus_tag="P5079_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRY29499.1"
                     /translation="MNFPIPNPLKRIVLGAAAVGLFFSGAARAEDSLVADFDAASPET
                     FAFKDEKGSVLNVSAAYTDPKKGKYTGIRYKIVSGGWGGWGIALKGFDASGFKYLALD
                     LRGDRGGETFEIGLRDKGGQEKKFPIAQFTEISPDWKEVLIPLAQFTGINLSALDNVN
                     IGLNEDHGAGKVSLDNVRFTGARESAAGDLSAAPTLANRTLVDGFERTTPADAYQVFQ
                     GDDSTLNLTSSRIVHDGDYAMEMEYQFSTTRPWGSWVSAQRNLRDPLDWTGVQEVKIW
                     AKGDGSENALRFRFVEADGETWEYTDSEILRLTKWTLLTMPVSAFTLVEPTKTDGQRG
                     LSAVRAYHIDILSPTARSSTGAKTTAGRIQLDQLYVLGERVKAVQAAPPAAAPGRAAG
                     LSNVDFSVLAYTEYFFTPEEKSQVNHYAKL"
     gene            complement(1263..3320)
                     /locus_tag="P5079_05605"
     CDS             complement(1263..3320)
                     /locus_tag="P5079_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY29500.1"
                     /translation="MTPTLSSAQSRPSVSAEDAQYLTDLARDTWACLDALVHPQTGLP
                     YDNNDPARKYTSVSNIGLYAADLAGAVELGLLPRDEARKRAEKLLASLEKFKHWKGFC
                     QSWNNLETLSPSPEDPWISVLDSGNLAGGLLVIKQAFPELRDRAGKYLDAMDWSAFYD
                     PATHTLYGGANMTTGALNKDWKLNLLGSDSRLASFLAIGAGKIAPAHWDTLSRGMETR
                     YGLDYLAPGWQGGGLFMQYISGLFVDEEGTLPGLSAARFAYVQMLHAQLIQSPAWGWS
                     ASDSPKDGYLGWQAIQDKIVTPHASALPLRFFPRECVANLRRLEQSGARAPTMVNGTP
                     KKYGFRDALDVTTAQVTDTYLMLDQSMLFLSIVNALKDGAIYKYAAKDPSVQKARERI
                     ADYQGKNLRREEFWKELASLAPRGLTLSLRTLETTTDFAPGEKTTVPVFSVGKADPAS
                     RLLWRVMDAKTGRYGPRGETSWPAAEIRFKSPAADGLYLFQAAIFGPGGRAATEDSFT
                     FRVSPPGAAARKEPVEDLPAVSWQDHGANWRARPPVAGDDWTVNTAPAARAGATKVGP
                     VVQLTFSFPKGEWVEMGQNLVDPSRLKRITFLHRCSGTPARLEIKFVDTDGTVFGQVR
                     PLRPAADWQAAVVDLSRLKHFWGGNGVLNTAEGLKLFFAVSGPDGGNGALEIKSLQGH
                     FEK"
     gene            complement(3373..4662)
                     /locus_tag="P5079_05610"
     CDS             complement(3373..4662)
                     /locus_tag="P5079_05610"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRY29501.1"
                     /translation="MKRSFLAAATLFILGGFSASPARAAVVTIDDFNSGKFQNALGNA
                     AGAWGDPSDKTAGCNAALDDKVFYGDAGLSLRLDYDVESSKQNVAIPTNVSVQNPAQV
                     RQDVFNGYFSLLKQFDARPYRYLVFWVKGDTKRGYTRSFKVELKDPQNSAGVLVDTVT
                     DRWQKIVLPLEKFTAIKDWSRLKEFVIVFDGNLVTRKEGTLYLDDLQFTDTLGEPART
                     GTAQRRAHKAAFMKKPPNLNTSLQDWPESAYVELGKGKEFLESGAVTNSADLDAWFAV
                     SWDKDFLYVAVDVSDNDVLNRNAGEHIWKDDLVELYIDPTDEGLTWGSDRYFQLGFSP
                     DSADGGPVRWAWFQKRAPADDEVKSVIKKYADGYQMKLAVAWNFLGLRPAPGQTIGFS
                     LAVHDRDTQDRTPEAKLNWDMKPASKTLFHLGRLTLK"
     gene            complement(4659..5603)
                     /locus_tag="P5079_05615"
     CDS             complement(4659..5603)
                     /locus_tag="P5079_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29502.1"
                     /translation="MGKTQGAPSKFSSVWTNIRIPLFHYSTIPLFLTISLFHYFTISP
                     SFAAFEPLGYGAAAKGMGGAYSALAEDGSAAYWNPAGLAFVKAPHITSAYEDLYGLGL
                     LRYTGMGYTQPKLGGGTASFQYLRLQTVGEASFFTYAENTYLLAYGRHLYGPLFGGVA
                     TRFYSANSEQKASAIGYDAGLMFSPSLEDRFRLSVALQDANEPRLRWNGGATDVLAPT
                     LRTGALLRLGPVSDFTAEHSRRRREKSCYRAGMAHHLMGRRFVLRAGVHKTVGQDSWN
                     LALGGGLSFKRLRLDYAWDHHETLGDSQTFSLSFRFGP"
     gene            complement(5639..7558)
                     /locus_tag="P5079_05620"
     CDS             complement(5639..7558)
                     /locus_tag="P5079_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRY29503.1"
                     /translation="MRRRTTLFLLGITLLAAAPLGATVIDTFDNTSFWASFADSAGAT
                     ASTGVVAGADGNAVQLTYDLTHGGFAGLTGTVTSQNLISEGANAVKFQVRATGVANTL
                     ELKFIDTDSSTTTDTCDKMDYKQLLNPDGAWREITVPFSDFKLFPDGNKSFNVGSAQR
                     LGIVVTKDNGTTGAGTVYVDDFRLCKLAGAASTIDSFETANTNARGLGATFFSDGVSG
                     GYAIYVTSQAVTGSKALELHYSIAAGTGRYAGIAEVLGGLVAANGNERLEFYAKGAAG
                     NEPLKIEIWHTNDTAIVSRSLSSYGTITTSYQKFSIPLSDFTGLDLKSITQIKLIFTD
                     GAGSGVVYLDDVAVVSPETETKNLRMLDDFQLPYSQIPYITASHRDASLTPSTLPDTT
                     PIGAQPDNRVLRLDYELKASTETPWVVLERKLRPNLLAEPALRFRFAGTGGNNDLEVK
                     LTDSDGTVYLRKFVAVTNTNGAWKTASVPVTDFGLNASGTDGILTLTDIVSIVFGVAR
                     GEKKSGTFTLDLVESAPGEAFRKDGVGAVLRSVSVPDNPFSPNDDGIKDTARFVLALS
                     RNARITLRIYSLRGVPLKTFDAGELAAGDHTLVWDGIDADGRRVANGLYLFHLEAEGS
                     NGDKDFFKEIIGVMR"
     gene            complement(7560..8558)
                     /locus_tag="P5079_05625"
     CDS             complement(7560..8558)
                     /locus_tag="P5079_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29504.1"
                     /translation="MMNSKMVNGEMGKTQKTQSKFSPVWTSFRFPLVLAISLFHYFTI
                     SLSFAAFKDPGYGARPAGMGNAFTAVADDVNAALFNPAGLAQIPSRQMAFSHAKPFTG
                     LDDVNLNLNFFSAVLPVGDLGSCGVAWSNLVAPVYRENTVTLSAASSLNRWLPDMKPY
                     LAVGVNVNALQNRFDLDDRTAGDSVFANGRSENAVAFDLGLYMKPDPDRLPGLTLGAM
                     GKSLNQPGLGLVETEQLKRELAGGAAYSFEKLTLSADLVRRDDVDTWRAGVESWFRQQ
                     TLGLRAGANSTAASAGFTVLFRAGRQAGIFLDYAYSIPFQVEDTAGSHRLALGLVF"
     gene            complement(8565..>10487)
                     /locus_tag="P5079_05630"
     CDS             complement(8565..>10487)
                     /locus_tag="P5079_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29505.1"
                     /translation="TRAGYAWVDNFNDTAEPSAFGFNSSTYIAGGSGTDLTHTYNSSN
                     YGFAPSGFQSIWTAGTISPTDAAVSLNMDGTTGNSLGVDIRGCDKLFFNVKGEGAANT
                     NLGVGIDRDSVGGKIHPSTVTLTTSWQSVTKTISEFGLDSTTDITKVEFWFINSNAAK
                     GPPINNTVSQTIYVDDIVFYDSIAPTAPSALADNGTAVADRHVFTSGSHTLTFTAPTA
                     GADETLERVFFAHDGNSGGTSWYIVYDATDTTSAPHSFTWDTSSLAEGTSYQVLIGAM
                     DVAGNQSTLAKSNIILLGTDTTAPTLTHTAVTEAYVNQPVAISISISDNKHAGPATLY
                     YRAKGTTTYTAASFDIGHPYPAAYAGSVTIPAAAMTSAGLEYYIETSDLINTTRFASA
                     AAPQTVTVKTNAQTSVGTTGGSVTLPDGNASDGETFVSIPEGALAQTVVVTIAAKNVA
                     AAPDGTTVGRQYALAAYEFGPSGTTFLKPVTLTLLYSDADQNGFVEDPATGLNTAIAE
                     TELRVFVHDGFQWQNLGGTVDAAKNTVTAKTMHFSVFGVFPGAATKDQLRPPQKIITP
                     GASPGVNDQLTFGGGADGAKIEIFDVTGRSIRRLENLTTWDGHDNRGDLAESGVYLYQ
                     AEIDGQKFSGTVTVAR"
BASE COUNT         1780 a         2967 c         3697 g         2043 t
ORIGIN      
        1 gagtttggcg tagtggttca cctggctctt ttcttccggc gtgaagaaat actcggtgta
       61 ggccagcacg ctgaaatcca cgttgctgag acccgcggcg cggccggggg cggcggccgg
      121 cggggcggcc tgcacggctt tgacgcgttc ccccaacacg tagagctggt ccagctggat
      181 gcggcccgcg gtggtcttgg cgccggtgct ggaacgcgcc gtggggctga ggatgtcgat
      241 atgataggcg cgcaccgcgg acaacccccg ctggccgtcg gtcttcgtgg gctccaccag
      301 ggtgaaggcg gagaccggca tggtgagcag ggtccatttg gtgagccgca gtatctccga
      361 atccgtatat tcccaggttt cgccgtcggc ctccacgaac cggaagcgca aggcgttttc
      421 ggaaccgtct cccttcgccc agatcttcac ttcctgcacg ccggtccagt ccaacggatc
      481 gcgcaggttg cgctgggcgc tgacccagga cccccacggg cgcgtggtgg agaactgata
      541 ttccatctcc atggcgtaat cgccgtcgtg cacgatgcgc gaagaggtga ggttgagggt
      601 ggagtcgtcg ccctggaaca cctggtaggc gtcggcgggg gtggtgcgtt cgaaaccgtc
      661 caccagggtg cggttggcca gggtgggggc ggcgctcagg tctccggcgg cggactcgcg
      721 ggcgccggtg aatcggacgt tgtccaggga caccttcccg gcgccgtggt cctcgttgag
      781 gccgatgttg acgttgtcca gggcggaaag gttgatcccc gtgaactggg cgagcgggat
      841 gagcacttct ttccagtcgg gcgaaatctc cgtgaactgc gcaatgggga actttttttc
      901 ctggccgccc ttgtccctca gcccgatctc gaaggtttct ccgccgcggt ccccccgcag
      961 gtccagggcg agatatttga acccgctggc gtcgaaccct ttgagggcga tcccccagcc
     1021 gccccagccg ccggagacga tcttgtagcg gatgccggtg tatttcccct ttttcgggtc
     1081 ggtgtaggcc gcgctgacgt tcagcacgct gcctttctcg tccttgaacg cgaatgtttc
     1141 cggggaggcg gcgtcgaaat cggcgacgag ggagtcctcc gcccgcgcgg cgccggagaa
     1201 aaacaggccg acggccgcgg cgccgaggac gattcgtttg agggggttcg ggatcgggaa
     1261 gttcattttt caaaatgtcc ttgcagggat tttatttcca atgcgccgtt gccgccgtcc
     1321 gggccggaga cggcgaaaaa gagtttcagg ccttccgccg tgttgaggac gccgttgccg
     1381 ccccaaaaat gtttgaggcg ggacaggtcc accaccgcgg cctgccaatc cgccgccggg
     1441 cggaggggcc gcacctgtcc gaacacggtg ccgtcggtat cgacgaattt gatctccaaa
     1501 cgggcgggcg tgccggaaca gcgatggagg aaggtgatcc gtttgaggcg ggaaggatcg
     1561 accaggttct ggcccatctc cacccactcg cctttcggga aagaaaaagt cagttgcacg
     1621 acggggccga ccttcgttgc tccggcccgg gcggccgggg cggtgttcac ggtccaatcg
     1681 tcgccggcga cgggcggccg ggcgcgccag ttcgccccat ggtcctgcca ggagacggcc
     1741 ggaaggtcct ccacgggttc cttgcgcgcc gccgcgccgg gcggagacac gcggaacgtg
     1801 aaagaatctt cggtggccgc gcggccgccg ggaccgaaga tcgccgcctg gaagagatag
     1861 agcccgtcgg cggccggaga cttgaatcgg atctcggcgg ccggccagga ggtttccccc
     1921 cggggcccgt agcggccggt tttggcgtcc atcacgcgcc agagaagtcg agaggcgggg
     1981 tccgccttgc cgacggaaaa aaccgggacc gtcgtctttt caccgggagc gaaatccgtc
     2041 gtcgtttcga gggtccggag cgagagggtc agcccgcggg gagcgagaga ggcgagctct
     2101 ttccagaatt cttcccgccg caggttcttt ccctggtaat cggcgatccg ttcgcgggct
     2161 ttctgaaccg aagggtcttt cgccgcgtac ttgtaaatgg cgccgtcctt cagcgcgttg
     2221 acgatgctga ggaagagcat ggactggtcc agcatcagat aggtgtcggt cacctgggcg
     2281 gtggtcacgt cgagcgcgtc ccggaaaccg tattttttcg gcgtgccgtt caccatcgtt
     2341 ggggcgcgcg cgcccgactg ttccagccgg cgcaggttgg ccacgcattc ccttgggaaa
     2401 aaacggagcg gaagcgccga ggcgtgcggc gtgacgatct tgtcctggat ggcttgccag
     2461 cccagatagc cgtccttggg cgaatccgag gcggaccagc cccacgccgg cgactggatc
     2521 agttgcgcgt gaagcatttg gacgtaggcg aaccgggcgg ccgagaggcc ggggagcgtg
     2581 ccctcttcgt ccacgaacag gccggagatg tattgcatga acagtccgcc gccctgccag
     2641 ccgggcgcaa gatagtccag cccgtagcgc gtttccatgc cgcgggagag cgtgtcccaa
     2701 tgggccgggg cgattttgcc ggcgccgatg gccagaaaac tggccaggcg ggaatcgctt
     2761 cccagcaaat tgagcttcca gtccttattc agggcgccgg tggtcatgtt ggcgccgccg
     2821 tagagcgtgt gagtggcggg atcgtagaac gccgaccagt ccatggcgtc caggtatttc
     2881 cccgcccggt cgcgcagttc ggggaaggcc tgcttgatga ccagaaggcc gccggcgagg
     2941 ttgccggaat cgagcacgga aatccaaggg tcttccgggc tcggcgagag cgtctcgaga
     3001 ttgttccaac tctggcagaa ccctttccag tgcttgaatt tttccaaaga ggccagtaat
     3061 ttttccgccc gtttcctcgc ctcgtctcgc ggcagaaggc ccagttccac ggcgcccgcc
     3121 aggtcggcgg cgtagaggcc gatgttggag acggaggtgt attttcgggc ggggtcgttg
     3181 ttgtcgtagg gcaggccggt ctgtgggtgg accagggcgt cgagacaggc ccaggtgtcg
     3241 cgggccaggt cggtcaggta ttgcgcgtcc tcggcggaga cggacggccg cgactgagcc
     3301 gacgaaaggg tcggggtcat gatcaggagg cctcccagaa atccacgaaa aaatcttccc
     3361 gccgtcatgg cgctatttca gggtcagccg acccaaatgg aagagggtct tacttgccgg
     3421 tttcatgtcc caattgagct tggcttcggg cgtgcggtcc tgggtgtcgc ggtcgtgcac
     3481 ggccagggag aaaccgatgg tctggcccgg cgcggggcgc aggccgagaa aattccaggc
     3541 gacggccagt ttcatctgat agccgtccgc gtattttttg atgacgcttt tgacctcgtc
     3601 gtcggcgggg gcgcgtttct ggaaccacgc ccatctcacg ggcccgccgt cggccgagtc
     3661 gggagagaac cccagctgaa agtaacggtc gctgccccag gtgagcccct cgtcggtggg
     3721 gtcgatgtag agttccacca agtcgtcttt ccagatgtgt tcgccggcgt tccggttcag
     3781 cacgtcgttg tcggacacgt ccacggccac gtacaggaaa tccttgtccc aggaaacggc
     3841 gaaccaggcg tcgaggtccg ccgaattcgt caccgcgccc gattccagga actctttccc
     3901 cttgcccaac tcgacgtagg cggattcggg ccagtcttgg agcgaggtgt tcaggttggg
     3961 cggttttttc atgaaagcgg ctttgtgcgc ccgccgctgg gcggttccgg tccgcgccgg
     4021 ttcgccgagg gtgtccgtga actggaggtc gtccaggtag agggtgcctt ccttgcgggt
     4081 caccaggttg ccgtcgaaga cgatgacgaa ctctttgagg cgggaccaat ccttgatggc
     4141 ggtgaatttt tccagcggca ggacgatctt ttgccagcgg tccgtcacgg tgtccaccag
     4201 gacgccggcg ctgttctgag ggtccttcag ctccacttta aaggaacggg tgtatccgcg
     4261 tttcgtgtcg cccttcaccc aaaagaccag gtagcggtaa ggccgggcgt cgaactgttt
     4321 caagagcgag aagtagccgt tgaagacgtc ttgacgcacc tgggccgggt tctgcaccga
     4381 aacgttggtg gggatggcca cgttctgttt cgaactctcc acgtcgtagt ccaggcggag
     4441 ggaaaggccg gcgtcgccgt aaaagacttt gtcgtcgagc gcggcgttgc atccggcggt
     4501 tttatccgaa gggtcgcccc aggcgccggc ggcattgccg agggcgttct ggaactttcc
     4561 gctgttgaag tcgtcgatgg tcacgacggc cgcgcgggcg ggactcgcgg agaacccgcc
     4621 gaggataaac agagtcgctg cggcaaggaa cgaacgtttc atgggccgaa ccggaaggaa
     4681 agagaaaagg tttgggaatc ccccaaggtc tcgtggtggt cccaggcgta atccagtcgg
     4741 aggcgtttga agctcaggcc gccgccgagg gcgaggttcc agctgtcttg gccgacggtc
     4801 ttatgcacgc ccgcccgcag gacgaaccgc cggcccatca gatggtgcgc catcccggcc
     4861 cggtagcagg atttctcacg ccgccgtcgg gaatgttccg cggtgaaatc gctcaccggc
     4921 cccaggcgga ggagggcccc ggtgcggagc gtgggggcca gaacgtccgt ggcgccgccg
     4981 ttccagcgca ggcgcggctc gttggcgtcc tgcagggcca cgctgagccg gaaacggtct
     5041 tcgagggagg ggctgaacat cagtccggcg tcgtatccga tggccgaggc cttctgttcg
     5101 gagttggccg aataaaagcg cgtggcgacg ccgccgaaaa gagggccgta caggtgccgg
     5161 ccgtaggcca gcagataagt gttttccgcg taggtgaaga aactggcttc tcccacggtc
     5221 tgaaggcgga gatactggaa gctcgcggtt cccccgccga gtttgggctg cgtgtagccc
     5281 atgccggtgt aacggagcag gccgagccca tagaggtcct cgtaggcgct ggtgatgtgc
     5341 ggggccttga cgaaggcgag cccggcgggg ttccagtagg ccgcggaacc gtcctcggcc
     5401 agggccgagt acgcgcctcc catgcctttg gcggcggcgc cgtatcccag cggttcgaac
     5461 gcggcgaaag atggtgaaat ggtgaagtag tgaaatagtg aaatggtgag aaatagtgga
     5521 atggtggaat agtggaatag tggaatacgg atatttgtcc aaacggagga aaatttggat
     5581 ggggcccctt gggtttttcc catttcacca ttcacttgct gccaccattc accgccgctc
     5641 accgcatcac cccgatgatt tccttgaaga aatccttgtc gccgttggaa ccttccgctt
     5701 ccaggtggaa gaggtagagt ccgttggcca cgcgccgtcc gtcggcgtcg atgccgtccc
     5761 agaccagggt gtggtccccc gccgccaact cgccggcgtc gaaggtcttg agcggcaccc
     5821 cgcgcaggga atagatccgc agggtgatgc gcgcgttgcg ggagagggcg agaacgaatc
     5881 gcgccgtgtc cttgatgccg tcgtcgttgg gagagaacgg gttgtccggt acgctcacgc
     5941 tccggaggac ggcgcccacg ccgtctttgc ggaacgcctc tccgggggcg gattccacga
     6001 ggtcgagggt gaacgtgccc gattttttct ccccccgcgc cacgccgaag acgatggaga
     6061 cgatgtcggt gagggtgagg atgccgtcgg tgccgctggc gttcagtccg aaatcggtca
     6121 ccggcacgga ggcggttttc caggcgccgt tggtgttggt gacggcgacg aacttgcgga
     6181 gatagacggt gccgtcgctg tcggtgagtt tcacttcgag atcgttgttc cccccggtgc
     6241 cggcgaaccg gaatcgcaac gcgggttccg ccaagaggtt cggccggagc ttgcgttcca
     6301 gcaccaccca gggcgtttcg gtggaggcct tcagttcgta gtccaggcgc agcacgcggt
     6361 tgtccggctg ggcgccgatg ggggtggtgt cggggagggt ggagggcgtg agcgaggcgt
     6421 cccggtggga ggcggtgatg tagggaatct gggaataggg cagttggaaa tcgtccagca
     6481 tgcggaggtt tttcgtctcc gtttcagggc tgacgacggc gacgtcgtcg aggtagacga
     6541 cccccgagcc ggccccgtcg gtgaagatga gcttgatctg ggtgatggat ttcaggtcca
     6601 atccggtgaa atcggagaga gggatggaga atttctggta gctggtggtg atggttccgt
     6661 aagaggagag ggaccgcgag acgatggcgg tgtcgttggt gtgccagatc tcgatcttga
     6721 gcggttcgtt gccggcggcg cctttggcat agaactccag gcgttcgttg ccgttggccg
     6781 ccaccagccc gcccaacacc tcggcgatgc cggcgtagcg gccggtgccg gcggcgatgg
     6841 aatagtggag ttccagggcc ttgctgccgg tgacggcctg ggaggtgacg tagatggcgt
     6901 atccgccgct cacgccgtct gaaaagaagg tggcgccaag cccgcgggcg ttggtgttgg
     6961 cggtctcaaa ggagtcgatg gtcgaggcgg cgccggccag tttgcagagg cggaagtcgt
     7021 ccacgtacac ggtgcccgcg ccggtggtgc cgttgtcttt ggtgaccacg atgcccagcc
     7081 gctgggcgga cccgacgttg aaggacttgt tgccatcggg gaaaagcttg aaatccgaga
     7141 acggcacggt gatctccctc caggcgccgt cggggttgag aagctgtttg tagtccatct
     7201 tgtcgcaggt gtcggtggtg gtgctgctgt cggtgtcgat gaatttcagt tccagggtgt
     7261 tcgccacgcc ggtggcgcgc acctggaact tcacggcgtt ggcgccttcg gagatgaggt
     7321 tctggctggt gacggtgccg gtgaggccgg cgaacccgcc gtgggtgagg tcgtaggtga
     7381 gctgcacggc gttgccgtcg gccccggcga ccacgccggt ggaggcggtg gcgccggcgc
     7441 tgtccgcgaa ggaggcccaa aaggaggtgt tgtcgaaggt gtcgatcacc gtggcaccga
     7501 gcggcgcggc ggcgaggagt gtgatcccta aaaggaagag cgtcgtccgg cggcgcatgt
     7561 cagaacacca gtcccaacgc caaccggtgg gaaccggcgg tgtcttccac ttggaagggg
     7621 atggaatacg catagtcgag gaatatcccg gcttgccggc ccgcgcggaa gaggacggtg
     7681 aaccccgccg aggcggcggt gctgttggcg ccggcgcgca agcccagggt ctgctgccgg
     7741 aaccaactct ccactccggc ccgccaggtg tccacatcgt cccgtcggac caggtccgcc
     7801 gagagcgtga gtttctcgaa actgtaggcc gcgccgcccg ccagctcgcg tttcagctgt
     7861 tcggtctcca ccaggccgag gcccggctgg ttaagggatt tccccatggc gcccaacgtc
     7921 aggcccggca ggcggtccgg gtcgggtttc atataaagac ccagatcgaa cgccacggcg
     7981 ttttccgagc ggccgttggc gaagaccgaa tcgccggcgg tccgatcgtc cagatcaaaa
     8041 cggttctgca aagcgttcac gttcaccccg acggccagat acggtttcat gtccggaagc
     8101 cagcggttca gggacgaggc ggcggagagg gtcacggtgt tctcgcggta gaccggggcc
     8161 accaggttgg accaggccac gccgcagctg ccgaggtccc ccaccggcag cacggcggaa
     8221 aaaaaattga ggttcaggtt gacgtcgtcc aggccggtga aaggtttggc gtggctgaag
     8281 gccatctggc gggaagggat ctgggcgagt ccggcggggt tgaacagcgc ggcgttgacg
     8341 tcgtccgcca cggcggtgaa ggcgttgccc atgccggccg gccgggcgcc gtaccccggg
     8401 tccttaaaag cagcaaagga gagtgaaatg gtgaaatagt gaaatagtga aatggcgagg
     8461 actagtggaa aacggaaact tgtccaaaca ggggaaaatt tggattgagt cttctgggtt
     8521 ttgcccattt caccattcac cattttacta ttcatcatta tttattaccg cgccacggtc
     8581 accgtgccgc tgaatttctg gccgtcgatc tcggcctggt agagataaac cccgctctcc
     8641 gccaggtctc cccggttgtc gtgcccgtcc caggtggtga ggttttccag ccggcggatg
     8701 gaacggccgg tgacgtcgaa aatctcgatt ttggccccgt cggcgccgcc gccgaacgtc
     8761 agctggtcgt tgacccccgg cgacgcgccg ggcgtgatga tcttttgcgg cggccggagc
     8821 tggtcctttg tggcggcgcc cgggaacacg ccgaaaacgg aaaaatgcat ggttttggcg
     8881 gtgacggtgt tcttggcggc gtccacggtg cctcccaggt tctgccactg gaagccgtcg
     8941 tgcacgaaca ccctgagttc ggtttcggcg atggcggtgt tcaggccggt ggcgggatcc
     9001 tccacgaaac cgttttgatc cgcatcggaa taaaggagcg tgagggtgac cggtttcaga
     9061 aaggtggttc cgctgggtcc gaactcatag gcggccaagg cgtattgccg tcccacggtg
     9121 gtgccgtcgg gggcggcggc gacgttcttg gccgcgatgg tcaccacgac cgtctgcgcc
     9181 agcgcgcctt cgggaatgga gacgaacgtt tccccgtcgg aagcgttgcc gtccggcagg
     9241 gtgacggacc cgccggtggt ccccacgctg gtttgggcgt tggttttcac cgtcacggtc
     9301 tgcggcgcgg cggcgctggc aaaacgggtg gtgttgatga ggtcgctggt ctcgatgtaa
     9361 tactccagcc cggcgctggt catggcggcg gccgggatgg tcacgctgcc ggcgtaggcg
     9421 gcgggatagg ggtggccgat gtcgaaggag gcggcggtgt aggtggtggt tcctttggcg
     9481 cggtaataga gcgtggcggg tccggcgtgt ttattgtccg aaatggaaat ggaaatcgcg
     9541 accggctggt tgacgtacgc ttcggtgacg gccgtgtggg tgagcgtcgg cgcggtggtg
     9601 tcggtcccca ggaggatgat gttggatttc gccaaggtgg attggttgcc cgccacatcc
     9661 atggcgccga tgagcacttg gtaggaagtc ccctcggcga gggaagaggt gtcccaggtg
     9721 aaactgtggg gggcggaggt ggtgtcggtg gcgtcgtaga cgatatacca gctggtcccg
     9781 ccggagtttc catcgtgggc gaagaagacg cgttcgaggg tctcgtccgc tccggcggtg
     9841 ggcgcggtga aggtgagggt atggcttccg ctggtgaaga cgtgtcggtc ggccaccgcg
     9901 gtgccgttgt ccgccagggc ggaaggcgcg gtgggggcga tactgtcgta gaaaacaata
     9961 tcgtccacgt aaatcgtttg ggaaacggtg ttgttgatgg gagggccctt ggcggcattg
    10021 ctgttaatga accaaaactc cactttagta atgtctgtcg tgctgtccaa tccgaactcg
    10081 ctgatggttt tggtcacaga ctgccaggag gtggtgagcg tgacggtgct gggatggatt
    10141 tttccaccaa cggaatctcg atcgatacct acgccgaggt tggtgttggc cgctccttcc
    10201 cctttgacgt tgaaaaagag tttgtcgcac ccgcgaatgt ctacccccaa tgaattgcca
    10261 gtggtcccgt ccatgttgag ggacactgcg gcgtcggttg gcgagatggt tcctgcggtc
    10321 caaatgctct gaaatccact gggggcgaac ccgtagttgg aactgttata ggtatgagtg
    10381 agatcggtcc cggagccgcc agcgatgtag gtgctactgt tgaacccgaa ggcactgggt
    10441 tcggcggtgt cgttgaagtt gtccacccag gcgtagccgg cgcgcgt
//
LOCUS       DAOTFD010000068         9909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4827_length_9909_cov_4.185610, whole genome shotgun sequence.
ACCESSION   DAOTFD010000068 DAOTFD010000000
VERSION     DAOTFD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_9909_cov_4.185610"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1826
                     /locus_tag="P5079_05635"
     CDS             159..1826
                     /locus_tag="P5079_05635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29506.1"
                     /translation="MLKERPGKIPAAPLLLAGAAAAWLAVNPTGDFPLNDDWAFASPV
                     RHLLQDNRLVLSDWTSPLAFPHLLTGFLLSKIFGFSHTVLRLSTLLWGVLAVYFLNRL
                     LRDEGVSPAPRRLACGLLAFHPVAFVLSFTYHTDVPFLALALGAIYFYLRADRTGNAR
                     DEIFASLLACGAALTRQIGLMIPLSATLWSLWRSYRSAGSLTATFSSLRWGRLARFWA
                     IPAFGVALFYAWFLHVHGPTWAHTVYVTQGTLRHAAHFLPFATETLKRTLDSLLYTAL
                     FAVAYFFIPAERSQRRSLGLDALFLAAAAALTVYLLRRGLFPYLENVYHPRGLGTMTV
                     YEGNRWKPAFLWEQVWLWKTHTALAAVSLWALLRRHLPRRPTETEVKMLVFAVPLAAA
                     TLVGAKYFDRYLLEWHLVFLLFFSVRFQKTRVPLLAWGTLLIWAVVSVAGTQDYFAWN
                     RAKWELGRVAARTLHLQPREIANGFDWNAHWKYESNMAELKKRKPLQEIGEWEWETME
                     PIRGVISYSPHLLPMEQRRMEKTYFSLLSPRGAVLYFYGRDRRPETP"
     regulatory      1877..2079
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2172..3014
                     /locus_tag="P5079_05640"
     CDS             2172..3014
                     /locus_tag="P5079_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRY29507.1"
                     /translation="MIRRLRLIATMSAVALLGARALAEARERRYVSLAPSTTEILFAL
                     GVDDELAGVSLLCEHPPAARTKPKVGSFSRPDVEKILSLRPDIVFCTGTEQAAAAANL
                     KKLGLTVYVSNPTSFQELFDSIQGIGRRIDRQRQADTLIAAMRGEINRVTAEVEQVPP
                     DKRPKVFVEFWHTPLMTAGKGSFVDELIHLAGGINIAGDLGAPYGNISAEEVIRRNPD
                     FIFLTSMDGKNNVRLVKESWGWDKISAIVNDRVYGDIDPDLVLTPGPRLVDGLRELHR
                     RLYP"
     assembly_gap    3187..3196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3197..3957
                     /locus_tag="P5079_05645"
     CDS             <3197..3957
                     /locus_tag="P5079_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY29508.1"
                     /translation="LQAILRNPLAEPYLLGTSSGAGLGAILAVVLGLGQAALPPAAFL
                     GALVSAALVYGIARQGYRIPVPSLILSGVVVSVAFSGIIVFVVTLSANEAVHGLLWWL
                     WGSLDVYDAKLLFYVCLLVLFGCATIFLFSQDLNALSIGEEDAVHLGIRAETIKTVMY
                     LLTSLLTAGLVCATGIIGFVGLIVPHMMRLVVGPNHKILIPASCLAGAAFLVGCDTLA
                     RTAFAPAEVPIGVVTAVIGAPIFIVLLKTRQRVP"
     gene            3954..4751
                     /locus_tag="P5079_05650"
     CDS             3954..4751
                     /locus_tag="P5079_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29509.1"
                     /translation="MSVLLKADQVWGGYSQAPVVKGVSLEIRRGEFVGLIGPNGSGKS
                     TLLKLMTRVLSPQKGTVSYEGQNIADMALKDLARKTAFVPQDTWVHFPFTVEEVVLMG
                     RVPHLRRLQSETKKDFSIADDALTLTGARHLKGQEIDKLSAGDRQRVIVAKALAQEPE
                     LLFLDEPTSHLDIGHQVRLLDMLRDLNREKKLTVLIVLHDLNLAGEYCDRLLLLEDGR
                     LFREGAPREVLTYANIETVYKTVVVVKENPISSKPYVILASGKQLRR"
     gene            4748..5332
                     /locus_tag="P5079_05655"
     CDS             4748..5332
                     /locus_tag="P5079_05655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY29510.1"
                     /translation="MRLVLIRHGETAGNRRRQYIGVTDQDLNARGFSQARNLRRRFHR
                     LKVDDVYSSDRRRAARFARLVFPGRRVRTTAGLREMDFGVMEGLTYRDGMAKYPKAYG
                     NWLTDPARHRLPRAERMADFKKRVLRALKDIVASQKNGTVAVVSHAGPIRLMINRLVR
                     ENFWGTRVPPAGVHLIDFRKGRGKLVPVRGKSHG"
     gene            5325..>5565
                     /locus_tag="P5079_05660"
     CDS             5325..>5565
                     /locus_tag="P5079_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRY29511.1"
                     /translation="MGKITFVLGGARSGKSRYAAGRIKNRNKKTAFVATGQALDGEMR
                     RRIAVHKKERPSHWTTFEEPLRLPSVLKKVVNGFDY"
     assembly_gap    5566..5575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5576..5841
                     /locus_tag="P5079_05665"
     CDS             <5576..5841
                     /locus_tag="P5079_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRY29512.1"
                     /translation="LTLWVSNMLLRRFSCETIQKDLAEVLRLLKKGKAASVVVANEVG
                     LGIVPANKLARNFRDVAGRVNQQVAEKADRVIFMVSGIPWRIK"
     gene            5841..6896
                     /gene="cobT"
                     /locus_tag="P5079_05670"
     CDS             5841..6896
                     /gene="cobT"
                     /locus_tag="P5079_05670"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRY29513.1"
                     /translation="MDKLNSVIGNIPEPDQRLFEKTQKRLDNLTKPVGSLGRLEELAR
                     LIVSMTGREKPSLPNKVIFTLAADHGVTEEGVSAYPKEVTPQMVYNFLRGGAGINVLA
                     AHVGARVVVADVGVAQDLIPHPNLVIQKIGYGTRNMANGPAMTRNEAVAAVEAGVAIF
                     ETEYEKGVDIVGTGDMGIGNTTASSAITAVITGAAVKTVTGRGTGIDEGRLKNKVDII
                     EKALTVNKPAPADALEVLSKVGGFEIGGLAGVILAAAARKVPVMLDGFISGAAALIAC
                     GLAPKAKHYLIASHCSVERGHKILLDHLGLGPLLDLDLRLGEGTGAALGIGLAEAAVK
                     ILTQMATFEEAGVSGKS"
     gene            6899..7645
                     /gene="cobS"
                     /locus_tag="P5079_05675"
     CDS             6899..7645
                     /gene="cobS"
                     /locus_tag="P5079_05675"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRY29514.1"
                     /translation="MNSFLAALRFLTVIPVKSSWTKDPNGSRTLIYFPVVGWLLGLAL
                     AVLITRLSRFSGQGVAPAMVVVVALVVLTGGLHLDGLADTFDALLSWKSKDEMLSILR
                     DPHVGVMGILGLLCVLLLKIAFLSWVSVPHQTAALVLMCVLGRWGLVWSLFLFPYARP
                     EGKAKAFAEGINRKIFFGATLVALSLILFFWQWRGLIAGFAAALSSFLVGHAAKKKLG
                     GITGDTLGAGCELTETTVLLSVCLLERIPL"
     gene            7642..8316
                     /locus_tag="P5079_05680"
     CDS             7642..8316
                     /locus_tag="P5079_05680"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HRY29515.1"
                     /translation="MNEEPVASSWSGGKDSCLACYKALRAGHPIGYLLNFISRESRRA
                     CFHGLEADLIKLQAERVGIPLVQKEVSPDMKKYEEEFKEAVSGLRARGVKKMVFGDIY
                     LLEHQNWIERVCGELRVEAMEPLWNAPPEDVLNEFLDLGFKAVIVSCKADVLGKEFLG
                     RVLDKPVIDEFKARGVCPCGENGEYHTLVVDGPMFKKGIRIMESETVLKDGFWKHWFW
                     DIKKYQ"
     gene            8458..>9909
                     /locus_tag="P5079_05685"
     CDS             8458..>9909
                     /locus_tag="P5079_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY29516.1"
                     /translation="MRKTQRLTAVFGLCFGIIGNAMGAGYEMQPIIITASRVGENPGD
                     MTRKVDVITHNDLETSAARDLSDVLTKLTSVNISHYGGVAGTKNVRMRGGTAQQVLVM
                     VDGRPINNPRDGSVDLNTVPLDNVDRIEVLHGPASSLYGSQAMGGAVNVITKTPLPGK
                     PVTEISTRYGSFSNSLHRLSHGAQISRFGYQINGELAGTRGFREHGGYKSQDLNGKWT
                     YDLAARHKISLNLGANGDRADLPGEITAVDLDDEQRSVKNFADLAWNAELNDRIGVSA
                     RVYDNFDRLEFRENTAGALWDTAYEKTVHRTWVQGIDGNLRSRPFDADTVMVGVTFQR
                     NKNESMRTGTHDYSVRAGYLENHLELFDRLKLSGSVRVDDYSTFGSKTNPSAGFLFKF
                     TPRYRFRGLYSRSFRAPTFNDLYWPDTGWTKGNPNVRPEKGATKEVGIDNDLTDDLSV
                     SATYFRNDFKDLINWAPDGAVWTPSNVDAAEMDG"
BASE COUNT         1961 a         3072 c         2989 g         1867 t
ORIGIN      
        1 ctccgcgcga agatctatcc cacagagaag caaacgttct acgtgcggca cggggacatc
       61 tttccgctcc tctgtctcct cctgtcggcg ggagggatga tccttttcct gggcgcccgg
      121 gggcgccagt ggtggaagga gcgtcgtcgg tgaatccgat gttgaaagaa cgtcccggta
      181 aaataccggc cgccccgctc ctgctggccg gcgcggccgc ggcgtggctg gccgtcaacc
      241 cgacgggcga tttcccgctc aacgacgact gggccttcgc cagcccggtg cgccatctcc
      301 ttcaagataa ccgtctcgtt ctttccgatt ggaccagccc gctcgcgttt ccacatctcc
      361 tcaccggctt tcttctgtcc aagattttcg ggttcagcca caccgtcctg cgcctcagca
      421 ccctcctctg gggcgttctg gctgtttact tcctcaatcg ccttctccgg gacgaaggcg
      481 tgtctcccgc gccgcgacgg ctggcctgcg gcctgttggc cttccatccc gtcgccttcg
      541 tcctgagttt cacctatcat acggatgtcc cgttcttggc gctggccttg ggagccatct
      601 acttttacct tcgagcggac cgcacgggga acgcgcggga tgaaattttc gcttcccttc
      661 tggcctgcgg agccgctctg acccgccaga tcggactgat gatcccgctt tccgccacgc
      721 tctggagcct gtggaggagt tatcgttccg ccggctcgct cacagccaca ttttcctcgc
      781 tccgatgggg acgtctcgcc cgcttctggg ccattccagc gttcggggtg gccctctttt
      841 acgcctggtt cctgcacgtg cacggcccca cgtgggcgca caccgtgtac gtgacccagg
      901 gcaccctccg acacgccgcc catttcctcc cgttcgccac ggaaaccctg aagagaacgc
      961 tggactcgct cctttatacg gcgttgttcg ccgtcgcgta tttcttcatc ccggccgaac
     1021 gctcccagcg gcgttccctg gggctcgatg cgctcttcct tgccgccgca gcggcgttga
     1081 cggtttatct ccttcggcgc ggcctttttc cctatctgga aaacgtctat catccgcgcg
     1141 gcctcggcac catgacggtg tatgagggga accgctggaa accggctttt ctttgggaac
     1201 aggtgtggct gtggaaaacc cacaccgccc tcgccgccgt gtcgctgtgg gctctgctga
     1261 gacgccacct tccgcgccgg ccgacggaaa ccgaagtaaa gatgctggtc ttcgccgtgc
     1321 cgctggccgc ggccaccctg gtgggcgcca aatatttcga ccgttacctt ttggaatggc
     1381 atctcgtctt cttgctcttc ttcagcgtcc gttttcaaaa aacgcgggtc cccctgttgg
     1441 cctggggaac ccttttgatc tgggccgtcg tttccgtcgc cggcacacag gattatttcg
     1501 cctggaaccg cgccaaatgg gaactcggcc gggtggccgc ccgcacgctg cacctccagc
     1561 cccgcgaaat cgccaacggg ttcgactgga acgcccactg gaaatacgaa tccaacatgg
     1621 cggaactgaa aaaacggaaa cccttgcagg agatcggcga gtgggaatgg gagaccatgg
     1681 aacccatccg cggcgtgatc tcctactcgc cccatcttct tccgatggag cagcgccgca
     1741 tggagaaaac ctacttttcg ctcctgtctc cgcgaggcgc ggtcctctat ttttacggaa
     1801 gggaccgccg gccggagacg ccctaagcga cgtttttctt gacacgcttc ggcgtgggta
     1861 ttacaatgag tctgcaacaa atcataacgg cgcaggcgct cgagagagct taaaggggaa
     1921 ttccgcgctt ttgtcgaagg acaaaagcca atggaaacgg tcccgccgct gtagaggatg
     1981 acgaaattcg aaacggccat tgccccgcga ggggcgagaa ggcccgaaaa gtaggacgat
     2041 tcctcagtca gaagacctgc ctgcgccttc aacgatgtcg caagaacatc gccgaacatg
     2101 ggttcctgga aaaccgggac cgaacccctg gcctatgcgt gccggggttt tttattttta
     2161 tggggagtcc tgtgatcaga cgtttacgcc tcatcgcaac gatgtcggcc gtcgcccttc
     2221 tgggggcgag ggcgttggcc gaggcccgcg aacggagata tgtctccctg gcgccgtcca
     2281 ccacggaaat attgttcgcc ctgggagtcg acgacgagct cgcgggggtc agcctgctct
     2341 gcgagcatcc gcccgcggca cggacaaaac ccaaggtggg gtcgttcagc cggccggacg
     2401 tggaaaaaat tttgtccttg cggccggaca tcgtcttctg caccgggacg gaacaggcgg
     2461 ccgccgcggc gaatctcaaa aaactcgggc tgacggtcta cgtcagcaat ccgacgagct
     2521 tccaggaact gttcgattcg atacagggga tcggccggcg gatcgacaga caacggcagg
     2581 ccgacacgct gatcgccgcg atgcgggggg aaatcaaccg ggtcaccgcg gaggtcgagc
     2641 aagtcccgcc cgacaaacgg ccgaaagtgt tcgttgaatt ctggcacacc cccctgatga
     2701 cggcggggaa aggctctttc gtggacgaac tcatccatct ggccggcggg atcaacatcg
     2761 ccggcgatct cggcgccccc tacggcaaca tcagcgccga ggaagtcatc cgacgcaacc
     2821 ccgacttcat cttcctgacc tccatggacg gaaagaacaa cgtgcggctc gtcaaggaga
     2881 gctggggctg ggacaagata tcggcgatcg tcaacgaccg cgtctacggc gacatcgatc
     2941 ccgacctggt cctcaccccc ggtccgcggc ttgtcgacgg cctgcgcgaa ttgcatcggc
     3001 ggttatatcc gtgaaaattc ccccgaagca caaaatctcc ctgttcgtcc tcttgctgct
     3061 ggccgcggcg gtgacgggcc tcgtcaaggg cgcggtggag atccccgtct ccgaactgct
     3121 cctgccggaa aaccgacaac tcctttcgct ccggctgatg cgcgttctgg cggggatcgc
     3181 cgccggnnnn nnnnnncgct ccaggccatc ctccgcaacc ccctggccga accctatctt
     3241 ctgggcacct ccagcggcgc cggattgggc gccatcctcg ccgtggtgct gggcctgggc
     3301 caagccgcgc tcccgccggc ggcgtttttg ggggccctcg tcagcgccgc cctggtctac
     3361 ggcatcgcgc gtcagggata tcggattccc gttccatcgc tcatcctctc cggagtggtg
     3421 gtgtcggtgg ccttttccgg gatcatcgtc ttcgtggtca cgctttcagc caacgaagcg
     3481 gtccacgggc tgctgtggtg gttgtgggga agcctggacg tgtatgacgc caaactcctt
     3541 ttttatgtct gcctcctggt cctgtttggc tgcgcgacca ttttcctgtt ctcacaggac
     3601 ctcaacgcgc tcagcatcgg ggaggaagac gccgtccatc tggggatccg ggcggagacc
     3661 atcaaaaccg tgatgtatct cctgacgtcc ctgctgaccg ccggactcgt ctgtgcgacg
     3721 gggatcatcg ggttcgtcgg actgatcgtc ccgcacatga tgcggctggt ggtcgggccc
     3781 aaccataaga tcctgattcc cgcgtcttgt ctggcgggag ccgcctttct ggtggggtgc
     3841 gacaccctcg cccgcacggc cttcgcgccg gcggaggtcc ccatcggcgt ggtcaccgcc
     3901 gtcatcggcg cgcccatttt catcgtcctc ctgaagaccc ggcagagggt gccgtgagcg
     3961 tcttgttgaa ggcggaccag gtctggggcg gctactcgca agcgcccgtg gtcaaagggg
     4021 tgtccctcga aatccgtcga ggagagttcg tggggctgat cgggccgaac ggttccggaa
     4081 aatccaccct cctgaaactc atgacgcggg tgctgagtcc ccaaaagggg accgtctctt
     4141 acgaaggtca aaacatcgcc gacatggccc tgaaagacct cgcccgcaag acggccttcg
     4201 tcccccagga cacctgggtc catttcccgt tcaccgtgga agaagtggtg ctcatgggcc
     4261 gggtgcccca cctgagaaga cttcaatccg aaacgaagaa ggatttttcg atcgcggacg
     4321 acgccctgac gttgaccggc gcccggcacc tcaaaggaca ggagatcgac aaactgagcg
     4381 cgggagaccg tcagcgcgtc atcgtcgcga aagcgctggc ccaggaaccg gaactcctgt
     4441 tcctggatga acccacctcg catctggaca tcggccatca agtgcgattg ctcgatatgc
     4501 tgcgggactt gaaccgagag aagaaactca ccgtcctcat cgtgctgcac gacttgaacc
     4561 tcgccggtga atattgcgac cggctcctgt tgctggaaga cggccgcctc ttccgggagg
     4621 gcgcgccccg ggaggtgttg acgtatgcca acatcgagac ggtctataaa accgtcgtgg
     4681 tggtgaagga gaaccccatt tcttcaaaac cgtacgtgat cctcgcgtcc gggaaacagc
     4741 tgcgccgatg agactggttc tcatccggca cggcgaaacc gccgggaacc gccgccggca
     4801 atacatcggc gtcacggacc aggatttaaa cgcacgcggc ttttcacagg cgcgaaacct
     4861 tcgccggcga tttcacaggc tgaaagtaga cgacgtctac tccagtgaca ggcggcgggc
     4921 ggcgcgtttc gcccgcctcg tgttcccggg ccggcgcgtg agaacaacgg ccggcttgag
     4981 ggaaatggac ttcggcgtca tggagggact gacgtaccgc gacggcatgg cgaaataccc
     5041 caaggcctac ggaaactggc tgacggaccc cgctcgccac agactgccgc gcgcggaacg
     5101 gatggccgat tttaaaaaaa gggttttgag agccctgaaa gacatcgtcg cctcccagaa
     5161 gaacgggacc gtggcggtgg taagccacgc cgggccgata cggctgatga tcaacagact
     5221 ggtgcgagaa aatttctggg gaacgcgcgt gcctcccgcc ggagtccacc tcatcgattt
     5281 caggaaaggg cgggggaaac tcgtccccgt ccggggaaag agccatgggt aaaatcacgt
     5341 tcgtcctggg tggtgcacgg agcgggaaaa gccgctatgc cgccggccgg atcaaaaacc
     5401 ggaacaaaaa aacggccttc gtcgccacgg ggcaggcgct cgacggcgaa atgcgccgcc
     5461 ggatcgctgt ccacaaaaag gaacgcccct cccattggac aacctttgag gagccgttgc
     5521 gcttgccttc cgtgctgaaa aaagtcgtga acgggtttga ttatgnnnnn nnnnngtctg
     5581 acactgtggg tttccaacat gttactgcgt cgtttttctt gcgaaacgat ccaaaaggac
     5641 ctcgccgagg tcctgcggct attgaaaaaa ggaaaagccg cgtcggtggt cgtggccaac
     5701 gaggtggggt tgggcattgt cccggccaac aaactggcga gaaatttcag ggacgtcgcc
     5761 ggccgtgtga atcaacaggt cgcggaaaaa gcggaccggg tgatcttcat ggtttcggga
     5821 atcccgtgga ggattaaata atggacaaat taaacagcgt catcgggaac attcccgaac
     5881 cggaccaacg cctgtttgaa aaaacgcaaa agcgcctgga caacctgacc aagcccgtcg
     5941 gcagtctggg ccggttggag gaattggccc ggttgatcgt gagcatgacc ggccgtgaaa
     6001 aaccgtcttt gccaaacaag gtcatcttta cgctggcggc ggatcacggc gtcaccgagg
     6061 aaggcgtgag cgcctacccg aaagaagtga ccccgcagat ggtctacaac ttcctgcggg
     6121 gcggggccgg catcaacgtg ctcgccgccc acgtcggcgc gcgcgtggtg gtggcggacg
     6181 tgggtgtggc gcaggatctg atcccgcatc cgaacctcgt cattcagaaa atcgggtatg
     6241 gcacccgaaa catggcgaac gggccggcca tgacgcgaaa cgaggcggtg gccgccgtcg
     6301 aagcgggggt ggcaatattt gaaaccgaat acgaaaaagg cgtggacatc gtcggcacgg
     6361 gtgacatggg gatcggcaac accacggcct ccagcgccat caccgcggtg atcaccggag
     6421 ccgccgttaa gaccgtcacg ggacgtggaa ccggcataga cgagggacgc ctgaaaaaca
     6481 aagtcgacat catcgaaaag gctttgaccg tgaacaaacc cgcccccgcc gacgcgctgg
     6541 aggtgctgtc gaaagtggga ggttttgaga tcggcggact cgccggcgtg atcttggcgg
     6601 ccgccgcccg aaaagtcccg gtgatgctgg acggcttcat ctcgggcgcg gccgcgctca
     6661 tcgcctgcgg gctggcgccg aaagcgaaac attatctgat cgcctcccac tgttccgtcg
     6721 aaagaggcca caaaatcctt ttggaccatc tgggcctcgg cccgctgctg gatttggacc
     6781 tgcggctggg agaaggcacg ggcgccgcgc tggggatcgg cttggccgaa gcggccgtca
     6841 aaatcctcac gcaaatggcc accttcgagg aagcgggcgt ttccggaaaa tcctaaaaat
     6901 gaacagtttc cttgcggccc tgcggttttt gacggtcatc ccggtaaaaa gttcttggac
     6961 aaaggacccg aacgggtccc gaacgttgat ctatttcccg gtggtcggat ggctcctggg
     7021 tctcgcgctg gccgttctca tcacgcgcct gtcccgtttt tcgggccaag gcgtcgcgcc
     7081 ggccatggtg gtggtggtcg cgctcgtcgt cttgacgggc gggctccatc tcgacggcct
     7141 ggcggacacc tttgacgcgc tcctgagctg gaagagcaag gacgagatgt tgtccatcct
     7201 gcgcgaccct cacgtcggcg tcatggggat cttggggttg ctgtgcgttc tgctgcttaa
     7261 gatcgccttc ctctcctggg tgagcgtccc ccaccagacg gcggcgcttg ttttaatgtg
     7321 cgtcctgggc cgctggggac tggtgtggtc gctctttctt ttcccttacg cccggccgga
     7381 gggaaaggcc aaggcgtttg ccgaggggat caaccggaaa atatttttcg gagccaccct
     7441 ggtcgccctc tcgctcatcc tgtttttctg gcaatggcgg gggctgatcg ccggcttcgc
     7501 ggcggcgctt tcctctttcc ttgtcggcca cgccgcaaag aaaaaactcg gcggcatcac
     7561 cggcgacacc ctgggagcgg gatgcgaact gaccgaaacg accgtcttgt tgagcgtctg
     7621 cctcctggaa aggatccccc tatgaacgaa gaacccgtcg cctcgtcctg gagcgggggg
     7681 aaagacagtt gtttggcctg ttacaaggcg ctccgcgcag gacaccccat cggctatctt
     7741 ttgaacttca tttcccggga atcccggcgt gcctgtttcc acggcctgga ggcggaccta
     7801 ataaaactgc aggccgaacg ggttgggata cccctcgttc aaaaagaggt cagtccggac
     7861 atgaaaaaat acgaggaaga attcaaagaa gcggtgtccg gcctgcgagc gcgcggcgtc
     7921 aagaaaatgg ttttcggcga catctacctc cttgagcacc aaaactggat cgagcgcgtc
     7981 tgtggagagc tgagggtcga agccatggaa ccgctctgga acgctccgcc ggaagacgtg
     8041 ctcaacgaat tcctcgatct tggattcaag gcggttatcg tcagctgcaa ggcggacgtg
     8101 ctggggaagg agttcctcgg ccgcgttctc gacaagcccg tcatcgacga attcaaggcg
     8161 cggggcgtct gcccctgcgg agaaaacggc gagtatcaca ccctggtcgt tgacgggccg
     8221 atgttcaaga agggcatccg catcatggaa agcgaaacgg tcctcaagga cggtttctgg
     8281 aaacactggt tttgggacat caagaagtac caataaaagc agaagaaaac cgaacgggcg
     8341 aaggaagtcc tgatgcgatc tcaggcactg ttccgcaacg gtaaaccgtc gaaacccgac
     8401 gggagtccgg tccagcgtcc gttccggtaa gtactctcga gaaaagagga aaataccatg
     8461 agaaagacac aaaggctcac ggccgttttc ggcctgtgct tcggcatcat cggcaacgcg
     8521 atgggggccg gctacgagat gcaacccatc atcatcaccg cttcccgcgt cggcgaaaac
     8581 cccggcgaca tgacgcggaa agtggatgtc atcacccaca acgacctgga gacctccgcg
     8641 gccagggact tgtcggacgt tctgacgaag ctcacgtccg tgaacatcag ccattacggc
     8701 ggggtggccg gcaccaaaaa cgtccgcatg cgcggcggca cggcgcagca ggtgctggtg
     8761 atggtggacg gccgccccat caacaacccg cgggacggat cggtggacct gaacaccgtg
     8821 cccctggaca acgtggaccg catcgaggtg ctccacgggc cggcttccag cctctacggg
     8881 tcgcaggcca tgggcggagc ggtgaacgtg atcaccaaaa cgcctcttcc cggaaaaccc
     8941 gtcacggaga tctccacgcg gtacggctcc ttcagcaact ccctgcaccg tctctcccac
     9001 ggcgcgcaaa tatcccggtt cggttatcaa atcaacggcg agttggccgg gacgcgcggt
     9061 ttccgcgaac acggcggcta taaatcgcag gacctgaacg gaaaatggac ctacgacctc
     9121 gccgctcgtc ataaaatctc cctgaacctc ggcgccaacg gcgaccgggc ggacctgccg
     9181 ggtgagatca ccgcggtcga cctggacgac gaacaaagaa gcgtcaagaa cttcgccgac
     9241 ctggcgtgga acgcggagtt gaacgaccgg atcggcgtct ccgcgcgggt ttacgacaac
     9301 ttcgaccgtc tggagttccg cgaaaacacg gccggcgccc tctgggacac cgcctatgaa
     9361 aaaaccgtcc atcggacctg ggtccaaggg atcgacggca acctccgcag ccgtccgttt
     9421 gacgccgaca ccgtcatggt gggcgttacc ttccagcgga acaagaacga aagcatgcga
     9481 acgggaaccc acgactactc tgtccgcgcg ggatatctgg aaaaccacct cgaacttttc
     9541 gaccgcctga aactgagcgg aagcgtccgc gtggacgatt actccacctt cgggtccaaa
     9601 acgaatccga gcgcgggctt cctcttcaag ttcacgcccc gctatcggtt ccgcggcctg
     9661 tacagccggt cgttccgtgc gcccaccttc aacgacctct actggcccga cacgggctgg
     9721 accaagggca accccaacgt tcggccggaa aaaggcgcga ccaaggaagt ggggatcgac
     9781 aacgacctca ccgacgacct gtccgtcagc gccacgtatt tccggaacga cttcaaggat
     9841 ctcatcaact gggccccgga cggcgcggtg tggaccccct ccaacgtgga cgccgccgaa
     9901 atggacggt
//
LOCUS       DAOTFD010000069         9864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4853_length_9864_cov_4.181976, whole genome shotgun sequence.
ACCESSION   DAOTFD010000069 DAOTFD010000000
VERSION     DAOTFD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_9864_cov_4.181976"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="P5079_05690"
     CDS             complement(<1..625)
                     /locus_tag="P5079_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY29517.1"
                     /translation="MQSFADVFKIPELRRRVLFTLGIVAIYRVGAAIPIPGINAEALQ
                     QLFAQQSNTLLGFLDIFSGGALGRLSIFSMGIMPYINSSIIMSLLQGAHVIPYLDRLA
                     KEGEMGRKKLNQITRYFTLLLGLIQSFGLTTLIMRIPLPGDVPVVKDPNAMFIFVTVV
                     TLTTGTIFVMWLGEQITEFGIGNGISLIIFSGIVESLPSAVKNMFVDV"
     gene            complement(632..1090)
                     /gene="rplO"
                     /locus_tag="P5079_05695"
     CDS             complement(632..1090)
                     /gene="rplO"
                     /locus_tag="P5079_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRY29518.1"
                     /translation="MKLNTLRPAPGSRHRRKIVGRGEGSGHGGKGSTRGFKGQTARSG
                     DKAIHPGFEGGQNPLIRRIPKRGFNNKTFRRAFCVVNVGDLEAVFEAGTAVAPDLLRS
                     AGLANDKGPVKILGDGDLTKKFSVSAHAFSGSAKKKIEQAGGSTEILKAK"
     gene            complement(1121..1615)
                     /gene="rpsE"
                     /locus_tag="P5079_05700"
     CDS             complement(1121..1615)
                     /gene="rpsE"
                     /locus_tag="P5079_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRY29519.1"
                     /translation="MPRIDASTLNLKETVVAINRVAKVVKGGKRFSFNALVVVGDQAG
                     HVGAAMGKAKEVQAAIQKAVHQGKKNLIKVPLIGGTIPHEIIGTFGAGKVFLKPAAPG
                     TGVIAGGSVRAVLEAVGIKDILTKSLRTPNPFNVVYATLEALSALRTKEEVARLRNKE
                     VSAL"
     gene            complement(1646..2011)
                     /gene="rplR"
                     /locus_tag="P5079_05705"
     CDS             complement(1646..2011)
                     /gene="rplR"
                     /locus_tag="P5079_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRY29520.1"
                     /translation="MSVKTPAELQHFRKIRGRRTIFGTLERPRLSVYRSSKHIYAQVI
                     DDVNGRTLVAASTLDTAIAKEKKSMATVEAAKAVGKLLAERAKEKTIQKVVFDRGGRT
                     YHGRVKALADAAREGGLQF"
     gene            complement(2008..2580)
                     /gene="rplF"
                     /locus_tag="P5079_05710"
     CDS             complement(2008..2580)
                     /gene="rplF"
                     /locus_tag="P5079_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002794624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRY29521.1"
                     /translation="MSRLGKKPVPIPDKVKVAVNGRQVQVDGPLGKLTRTLPDGIGAQ
                     LQDKAVLVTRRDDTTQQKSLHGTLRKILLNMVDGVSKGFTKELRIEGVGYRAQQTGNK
                     ISFALGFSHNKEFEVPSGIKVAIDAKQTGLTVQGADKQLVGQVAAEIRKLKPPEPYKG
                     TGIRYADEHVRRKAGKAAVGATGGVGGAKK"
     gene            complement(2594..2989)
                     /gene="rpsH"
                     /locus_tag="P5079_05715"
     CDS             complement(2594..2989)
                     /gene="rpsH"
                     /locus_tag="P5079_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRY29522.1"
                     /translation="MTTDPIGDMFTRLRNANSARHERVDMPSSNLKKDIAHLLKEEGY
                     IADYKILTERRQKILRVFFKYMPDKSRVMQGVRRVSRPGLRIYRGCDDLPRVSGGLGM
                     AIISTSKGLLSDKQSRQQKLGGEVLGYIW"
     gene            complement(3018..3203)
                     /locus_tag="P5079_05720"
     CDS             complement(3018..3203)
                     /locus_tag="P5079_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRY29523.1"
                     /translation="MATYAAMAKMRKVQKFATRFRNRCRLCGRPRAFIRDFGLCRICF
                     RKLALRGEIPGVVKASW"
     gene            complement(3215..3790)
                     /gene="rplE"
                     /locus_tag="P5079_05725"
     CDS             complement(3215..3790)
                     /gene="rplE"
                     /locus_tag="P5079_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRY29524.1"
                     /translation="MTEKNNGYVPRLRTAYFQAVVPELMKRLSLTNPFQVPRLKKVVI
                     NMGVSDARENVKSIDVAADDLGVITGQKPEVRRAKKSISNFKLRQGMPIGVRVTLRGA
                     RMWEFFDRLVNIAMPRLRDFQGLNPHKGFDGRGNYNLGLNEQYVFPEINLDKSDKARG
                     MNITVVTSADKDASARELLTLLGMPFKRDSK"
     gene            complement(3804..4115)
                     /gene="rplX"
                     /locus_tag="P5079_05730"
     CDS             complement(3804..4115)
                     /gene="rplX"
                     /locus_tag="P5079_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRY29525.1"
                     /translation="MLHIRKKDKVVVLAGKDKGKQGDVVEVDLGKGRALVGKVNFVKR
                     HTRPTQTEPGGIREKEAYVPLSRLMLVCPKCTHPTRVKFDSLSDGTKARVCRNCGEMI
                     V"
     gene            complement(4115..4483)
                     /gene="rplN"
                     /locus_tag="P5079_05735"
     CDS             complement(4115..4483)
                     /gene="rplN"
                     /locus_tag="P5079_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRY29526.1"
                     /translation="MIQERSILRVADNSGARRVRVFRMLGGSRRRYAFLGDIVVCSVQ
                     DALADSTIKKGDVVKCVIVRTRKEKRRADGSYVRFDDNAGVLIDVNGEPRGTRIFGPI
                     ARELRERNYLKIISLAPEVV"
     gene            complement(4480..4794)
                     /gene="rpsQ"
                     /locus_tag="P5079_05740"
     CDS             complement(4480..4794)
                     /gene="rpsQ"
                     /locus_tag="P5079_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRY29527.1"
                     /translation="MTSPQPAEQRTKRKNLEGWVVSDRMDKTRVVKVLRRLRHALYEK
                     VLARTTKVHAHDEKNEAHVGDRVELEETRPLSKLKRWRVVKILEKARVGEAAAQSAEE
                     KA"
     gene            complement(4791..4994)
                     /gene="rpmC"
                     /locus_tag="P5079_05745"
     CDS             complement(4791..4994)
                     /gene="rpmC"
                     /locus_tag="P5079_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018581627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRY29528.1"
                     /translation="MAKKEKIDYRELSQDELRQRLVEAREKLFELRFQSTTAPLKNPH
                     LVSTTRREIARLLTVIKQKGVRV"
     gene            complement(5005..5418)
                     /gene="rplP"
                     /locus_tag="P5079_05750"
     CDS             complement(5005..5418)
                     /gene="rplP"
                     /locus_tag="P5079_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRY29529.1"
                     /translation="MLMPTRVKYRKSHRGRLKGRAKRGNTVAFGQYGLQALESHWITA
                     RQIEAARVTIMRYLKKGGKVWVRVFPDKPVSKKPAETRMGKGKGNPEFWVAVVRPGRV
                     MYELEGVTPEVAKEVLTLASHKLPIRCQVLMREEF"
     gene            complement(5650..6273)
                     /gene="rpsC"
                     /locus_tag="P5079_05755"
                     /pseudo
     CDS             complement(5650..6273)
                     /gene="rpsC"
                     /locus_tag="P5079_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(6350..6697)
                     /gene="rplV"
                     /locus_tag="P5079_05760"
     CDS             complement(6350..6697)
                     /gene="rplV"
                     /locus_tag="P5079_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRY29530.1"
                     /translation="MQAIAHSRFARYSYRKVGQVLGLIRGKAVPQAMALLPWVPRVAR
                     VLVEKTLRSAVANAGKGANPAGLRVSEAWVNHGPVLKRVRPMAMGGRAIYKRKTCHLT
                     IVVSDENKGGKNK"
     gene            complement(6713..7000)
                     /gene="rpsS"
                     /locus_tag="P5079_05765"
     CDS             complement(6713..7000)
                     /gene="rpsS"
                     /locus_tag="P5079_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRY29531.1"
                     /translation="MSRSTSKGPYVDQRLLGKIQKMKQSGEKKVIKTWARASMITPDF
                     VGFTLAIHNGKKFLPVFVSDQMVGHRVGEFAPTRFFKGHGAAHTKEATALT"
     gene            complement(7079..7918)
                     /gene="rplB"
                     /locus_tag="P5079_05770"
     CDS             complement(7079..7918)
                     /gene="rplB"
                     /locus_tag="P5079_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRY29532.1"
                     /translation="MPMKTFKPYTPSRRFIAVEDFSEISKKGPEKSLLEPLRKKGGRN
                     NTGMIMVRHHGGGHKRHYRVVDWKRDKAGVPAKVAAIEYDPNRTARLALLHYKDGEKR
                     YILQPAGLKVGDTVLSGPAAEIRPGNAMPLANLPIGTFVHNVELVPGRGGQMVRSAGA
                     AAQLMSRDNGFAQLKLPSGEIRMVPEICYATIGQVGNAEHENLTIGNAGRSRHMGVRP
                     HVRGTAMNAVDHPHGGGRGRSKGNNQPRSPWNQPAKGYKTRSPKLHDWMIVRRRSDAA
                     EQI"
     gene            complement(8008..8289)
                     /gene="rplW"
                     /locus_tag="P5079_05775"
     CDS             complement(8008..8289)
                     /gene="rplW"
                     /locus_tag="P5079_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRY29533.1"
                     /translation="MSVLRRPIVTERSTAMKEQNKYVFEVDPGATKGQVKEEVEAYFK
                     VDVLRVNTALMPGKMRRRMGPRGGYRSDWKKAIVTIKAGQEIKFAEPTA"
     gene            complement(8293..8958)
                     /gene="rplD"
                     /locus_tag="P5079_05780"
     CDS             complement(8293..8958)
                     /gene="rplD"
                     /locus_tag="P5079_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRY29534.1"
                     /translation="MPLSMDVLNVKGEKVSSVDLPEAVFGVKGSPALVHEVINAYLAN
                     RRRGTHSTKTRGEVSGGGVKPWKQKHTGRARSGSIRSPLWRHGGVTFGPKPRSYYQSV
                     PEAKRRLALKMILTDLVKDGRMKVVDAVSVPEPKTKHVAAMVKKLNAPGRTVMVLDQI
                     EPVFLRAARNMAGLRLCRVENLNSYDALQAEQLIFSKPALDKIVQRLNGDLAPAGPAA
                     GKE"
     gene            complement(8989..9801)
                     /gene="rplC"
                     /locus_tag="P5079_05785"
     CDS             complement(8989..9801)
                     /gene="rplC"
                     /locus_tag="P5079_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006691179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRY29535.1"
                     /translation="MEEQKTEVTATPPPADPAAQPAPANPVAPAEAPPTKSILGRKIG
                     MTRIYRPNGEFVPVTVVEAGPCPILQVKTVDSDGYAALQVGFGDAKEKNVNKALKGQF
                     AKAQVKPVRWVREIRLPKVEGFRPGQVIRVTNFSEGDVLDVSGYSKGKGFAGVVKRHR
                     FRGGPKTHGQSDRWRAPGTSGGQRPQRVFKGIRGPGHMGVDWVTVQRLTVEKVDADRN
                     LLLLKGSVPGPLDTCLTIRPTTRPMKAKKVPPAPKAKKAAKQPAAAKAPAKK"
BASE COUNT         1767 a         3225 c         2683 g         2189 t
ORIGIN      
        1 acacgtccac gaacatgttc ttgacggccg acggcaaaga ctccacgatg ccggaaaaga
       61 tgatcagcga gatgccgttg ccgatgccga attccgtgat ctgttccccc agccacatga
      121 cgaagatcgt tccggtggtc aacgtgacca cggtgacgaa gatgaacatc gcgttggggt
      181 ccttcaccac cggcacatcg ccgggaaggg ggatccgcat gatcagcgtg gtcagcccga
      241 aggactggat caaccccaac aacagagtga aatagcgcgt gatctggttg agcttcttgc
      301 ggcccatctc cccttccttg gccaaacggt ccagataagg aatcacatgg gccccctgca
      361 ggaggctcat gatgatggag gagttgatgt agggcatgat ccccatggaa aagatcgaca
      421 accggccgag cgccccgccg gagaaaatat ccaaaaagcc gagcagcgtg ttggactgct
      481 gagcgaacag ctgttgcaac gcctcggcgt tgatgcccgg gatcgggatg gcggccccca
      541 cccggtagat ggcgacgatc ccgagggtga acaaaacgcg ccgacgcagt tcggggatct
      601 taaaaacgtc ggcgaacgat tgcatgaaaa actatttcgc cttcagtatt tccgtcgaac
      661 cgccggcctg ttcgatcttc tttttggcgc ttccggaaaa ggcgtgcgcc gagacgctga
      721 atttcttcgt caggtcgccg tcgcccaaaa tcttcaccgg gcccttgtcg ttcgccagcc
      781 ccgccgatcg caggaggtca ggcgccacgg cggtgcccgc ctcaaagacc gcctccagat
      841 cgcccacatt gaccacgcaa aaggcacgcc gaaacgtctt gttgttgaaa ccccgcttgg
      901 ggatgcggcg aatgagcgga ttctgtccgc cttcaaaacc ggggtggatc gccttgtctc
      961 cggatcgggc ggtctgtccc ttgaacccgc gggtagaccc ttttccgccg tgaccgctgc
     1021 cttctcctcg gccgacgatc ttccgacggt gccgggagcc gggggcgggg cgcaacgtgt
     1081 taagtttcat catctcgaac tccccttata attctccgcg ctacagcgcg gatacttcct
     1141 tgttgcgaag acgggccact tcctctttcg tgcgcagggc ggacagcgct tcgagcgtgg
     1201 catagaccac attgaacggg ttcggcgtcc tcaaggattt cgtgaggatg tccttgatgc
     1261 ccaccgcctc caaaaccgcg cggacgctgc cgccggcgat aacgcccgtg ccgggggcgg
     1321 cgggtttcag aaaaaccttg ccggcgccga aagtgccgat gatttcatgc ggaatggtcc
     1381 cgccgatcag cggcaccttg atcaaattct ttttcccctg atgtaccgcc ttctgaatgg
     1441 cggcctgcac ttccttggcc ttgcccatgg ccgcgcccac atgccccgcc tggtctccca
     1501 ccaccaccag cgcgttgaag gaaaaccttt ttcctccttt gaccactttg gcgacacggt
     1561 tgatcgccac caccgtctcc ttcagattca acgtcgaagc atcaattctg ggcacaccgc
     1621 ctccttaaaa ggattaaaaa ccgttttaga actgcagacc gccctcgcgg gcggcgtcgg
     1681 ccagcgcttt cacgcggccg tgatacgttc gaccgccacg gtcgaacaca actttctgga
     1741 tagtcttctc cttggcccgt tcggcgagga gtttcccgac ggctttcgcc gcctcgacgg
     1801 tcgccatgga ttttttctcc ttggcgatgg ccgtgtccaa ggtggacgcc gccacgagcg
     1861 tgcggccgtt gacatcgtcg atgacctggg cgtagatatg tttcgacgac cgataaacgg
     1921 agagccgcgg acgctccagc gtgccgaata tcgtccgccg gccgcggatc ttcctaaaat
     1981 gctgcaactc tgcgggcgtt ttaacgctca tttcttggcc cctcccaccc cgccggtggc
     2041 gccaaccgcc gccttgccgg ccttgcgccg gacatgctcg tcggcatagc ggatccccgt
     2101 ccctttatag ggttcgggcg gtttcagttt tcgtatctcg gcggcgacct ggccgaccaa
     2161 ctgcttgtcg gcgccctgga ccgtcagccc cgtctgcttg gcgtcaatcg cgaccttgat
     2221 cccgctgggg acctcgaatt ccttgttgtg agaaaaaccc agcgcgaatg aaatcttgtt
     2281 gcccgtctgc tgggcgcgat accccacgcc ttcaatcctc agttctttcg taaatccctt
     2341 ggaaacgccg tccaccatgt tcaacaggat cttccgcagg gtcccgtgca gggatttctg
     2401 ctgggtggtg tcgtcgcggc gggtcaccaa cacggccttg tcctgcagtt gggcgccgat
     2461 gccgtccggc agggtccgcg tcagttttcc cagcggacca tccacctgga cctggcggcc
     2521 gttgaccgcc accttgacct tgtctggaat cgggaccggt ttttttccca aacgactcat
     2581 cgaatgtcgc tccttaccag atatatccga gcacttcgcc gcccagcttt tgctggcggg
     2641 attgcttatc ggacaacagc cccttggaag tggagatgat cgccattccc aggccgccgc
     2701 tgacgcgtgg aagatcgtca cagccgcggt atatccgcag gcccggccgg gaaacccgcc
     2761 gcacaccctg cataacccgc gatttatccg gcatatactt gaagaaaacg cggagaatct
     2821 tctgacggcg ttccgtcaga atcttataat cggcgatata gccctcctct ttcagtaggt
     2881 gggcgatatc ctttttcagg tttgaggaag gcatatccac ccgctcgtgg cgggcgctat
     2941 tggcattcct caaccgggta aacatgtcgc ctatcggatc ggttgtcatc tttctctgtc
     3001 ctccaaaaaa acgcggctta ccagctcgcc ttgacgacgc cggggatctc ccccctcaac
     3061 gccagtttcc gaaagcaaat acggcacaat ccgaaatcgc gaatgaacgc ccgcgggcgg
     3121 ccgcagagcc ggcaacggtt acgaaaacgg gtggcgaact tttgcacctt cctcattttt
     3181 gccatggcgg cataggtggc cattgtcgtc tcctttactt actgtcccgc ttgaacggca
     3241 tcccgagaag cgtcagaagc tcccgggccg aagcatcttt gtccgcggag gtcacgacgg
     3301 tgatgttcat cccgcgcgcc ttgtccgatt tatccaagtt gatctccgga aaaacatact
     3361 gttcgttcaa ccccaggttg tagtttccac ggccgtcgaa gcccttgtgc ggattcaatc
     3421 cctgaaagtc gcgcaggcgg ggcatggcga tgttgacgag ccggtcgaag aattcccaca
     3481 tcctcgcccc gcgaagcgtc acccgtacgc cgatgggcat gccttgcctc agcttgaagt
     3541 tggagatgga tttcttagcc cggcgcacct cgggtttctg gccggtgatc acccccaaat
     3601 cgtcggcggc gacgtcgatg gatttcacgt tctcccgggc gtccgaaacg cccatgttga
     3661 tgacgacctt cttcaaccgc ggcacctgga acgggttcgt cagagacagg cgcttcatca
     3721 gctccggaac cacggcctga aaatacgccg ttctcaaccg cgggacatat ccgttgtttt
     3781 tttccgtcat ttttccttgc ccctcagacg atcatttctc cgcagtttcg gcacacccgg
     3841 gccttggtgc cgtcggagag ggaatcaaat tttacccgcg tgggatgcgt gcacttggga
     3901 cagaccagca tcagccggga gagcggcaca taggcctctt tttcccggat gcccccgggt
     3961 tccgtctggg tcggccgggt atgccgcttg acgaaattca ccttgccgac gagagcgcgg
     4021 cccttcccca ggtccacctc caccacatct ccctgtttcc ccttgtcctt gccggccaga
     4081 acgacgactt tgtctttttt ccgtatatgc aacatcagac gacctcgggt gccaacgaaa
     4141 tgattttcaa atagttgcgt tcgcgcagtt cccgggcgat ggggccgaaa atacgcgttc
     4201 cccgcggttc cccgttgacg tctatcagca cgccggcatt gtcgtcgaac cgcacgtagg
     4261 agccgtccgc gcgccgcttc tccttgcggg tgcgcacaat gacgcatttg acgacgtccc
     4321 cctttttgat ggtggaatcg gccagggcgt cttggacgga acagaccacg atgtcgccta
     4381 aaaacgcgta gcgccggcgc gacccgccca gcatgcggaa cacccgcacc cggcgggcgc
     4441 ccgagttgtc cgccacgcgc aatatgcttc gctcctggat catgccttct cctccgccga
     4501 ttgggcggcc gcttcaccca ccctcgcctt ctccaaaatc ttcacgacgc gccaccgttt
     4561 caacttcgaa agcgggcgcg tctcctcgag ctccacgcga tcgccgacgt gcgcctcgtt
     4621 cttctcatcg tgagcgtgca ctttcgtggt gcgggcgagc accttctcat aaagtgcatg
     4681 cctcaggcgg cgaagaacct tgacgacccg cgttttgtcc atgcggtcgc tgaccaccca
     4741 cccctccaaa tttttccgct tcgtccgctg ctccgctggt tgaggcgatg tcacacgcgc
     4801 acccctttct gtttgatcac tgtcaaaagc cgtgcgatct cgcgccgcgt ggtcgaaacg
     4861 aggtgcggat tcttcaacgg cgccgtggtg ctctggaacc gcagttcgaa aagtttctca
     4921 cgcgcttcca ccagacgctg gcggagctcg tcttgagaca actcccggta atcgatcttt
     4981 tcttttttgg ccatgggtcg ccctctagaa ttcttcccgc atcaaaacct ggcagcggat
     5041 cggcaattta tgggaggcca gcgtgaggac ctctttggcc acctccggcg tgacgccttc
     5101 cagttcatac atcacgcggc cgggccgcac cacggccacc caaaactccg gattcccctt
     5161 cccttttccc atccgcgttt ctgcgggttt cttgctgacg ggtttgtcgg gaaacacgcg
     5221 cacccacacc ttgccgccct tcttcaggta gcgcatgatg gtgacgcggg cggcctcgat
     5281 ctggcgcgcg gtgatccaat gcgattccaa cgcctgaaga ccgtactgtc cgaaagcgac
     5341 ggtgtttccg cgtttggcgc ggcctttgag acggccgcga tgggacttgc gatatttgac
     5401 gcgcgtcggc atcaacatgc cgtcttactc cttcaagtcc gaacgacgac ggagcgtctc
     5461 gtcctcggat ttctcaatgt ctttcaagac cttctcctcg agcacctcgt cctccggccc
     5521 cgccggctcc accggcaaaa tacccgcgtc ttccgccgca ttggcggttt ccgccgcctg
     5581 tttggcgagc tcttccatct tctctttctc gatcaaacgg acgtcctcca tcaattcggc
     5641 gtcggttttc ttgaaaagtt ccttccggaa aatccagacc ttcacgccga tgcgccccat
     5701 ggtgatgtga gcctcggtga atccataatc gatatccgcg cggaaggtct gcagaggcac
     5761 gcggccctcc ttcaaccatt ccgtgcgggc gatctccgcc ccgcccagcc ggccggccac
     5821 ctggaccttg atgccgaggg cgccgccctg catggcccgc tcgatcgact ttttcatggc
     5881 ccgacgaaaa cccacctgct tttccaactg catcgccacg ccctcggcca ccagttgggc
     5941 gtccaattcc ggccgcttga tctccatcac gttgatggtg gtcttccggc ccgtcatcgc
     6001 ctccacggcg gtgcgaaggc cttcgatgtc ggcccctttc ttcccgatga cgatgccggg
     6061 ccgggcggtg aaaacattga cgcggagata gttccccgtc cgctcgatcc cgatcttgga
     6121 aacagccgcc agtttcagcc ggtccttcaa aaacgttcgg atcttgtggt cttcctcgat
     6181 caacgcgggc atctcgcgca tgttgaacca gcgggagtcc caatccttga tgtatccgag
     6241 gcgaattcct tttggatgaa ctttttggcc cataaatcaa agtgcgaagc gggaggtggg
     6301 aagcggaaag caacagttct tactttgccc gccgcgcctt tccgacttct tacttatttt
     6361 tgcccccttt attttcgtca ctgaccacga tcgtcagatg gcatgtcttc ctcttataga
     6421 tggcacggcc gcccatggcc atcgggcgga cgcgtttcag aacgggcccg tgattgaccc
     6481 aggcctcgga aacgcgaagc ccggccggat tggccccttt gcccgcgttg gccaccgcgc
     6541 tccgaagcgt cttttccacc aaaacccggg ccacgcgcgg cacccacggc aaaagcgcca
     6601 tcgcctgcgg cacggctttg ccgcggatca gtccgagcac ctgcccgacc ttccgataag
     6661 agtaacgcgc aaacctggaa tgagcgatcg cctgcatgtc tatcccttcg aattacgtga
     6721 gggccgtggc ctctttggtg tgggcggccc cgtggccctt gaaaaaccgc gtcggcgcga
     6781 attcacccac ccggtgtccg accatctggt cggacacgaa caccggcaaa aactttttcc
     6841 cgttgtggat cgccagcgta aaaccgacga aatccggagt gatcatgctg gcccgggccc
     6901 aggtcttgat cacttttttc tctcccgact gtttcatctt ctggattttc cccagaagtc
     6961 gttggtcgac gtaaggacct tttgaagtgg aacggctcat gtttttctaa actcctgatt
     7021 taaggttcaa ggtggagggc ccggaacgac ttcgggcttt cgacctttat ggactgtcct
     7081 aaatctgttc cgccgcgtcc gagcgccgac ggacgatcat ccaatcgtgc agtttcggcg
     7141 agcgggtctt atagcccttg gcgggctggt tccacgggga ccgcggctga ttgttgcctt
     7201 tcgatcggcc gcggccgcca ccgtgcggat ggtccacggc gttcatggcg gtgccgcgca
     7261 cgtgcggccg gactcccatg tgccgcgacc ggcccgcgtt gccgatggtc aaattttcat
     7321 gttccgcgtt ccccacctgc ccgatggtgg cgtaacaaat ctccggaacc atacggatct
     7381 cccccgaagg gagcttcaac tgggcgaacc cgttgtcgcg cgacatcaac tgggccgccg
     7441 ccccggcgga ccggaccatc tgaccgccgc gcccgggaac cagttccacg ttgtgcacga
     7501 acgtgccgat gggaaggttc gccaaaggca tcgcgttgcc cggccgaatc tccgcggccg
     7561 gaccggaaag gacggtgtct cccaccttca gccccgccgg ttgcaggatg taccgttttt
     7621 cgccgtcctt gtaatgaagc aacgccagcc gggcggtgcg attcggatcg tattcaatgg
     7681 ccgccacctt ggccgggaca ccggccttgt ccctcttcca atccaccaca cgataatgcc
     7741 tcttatggcc gccgccgtgg tggcgcacca tgatcatgcc ggtgttgttg cggccgcctt
     7801 ttttccggag cggctcaagg agggactttt ccggtccctt cttgctgatc tccgaaaaat
     7861 cctcgacagc gatgaaccgt cgggaggggg tatagggctt aaatgttttc atcggcataa
     7921 tattaaccca aatctgaaat ctgaaataaa aaatacgaaa gaaaccctgt tgcctttatt
     7981 tcgtatttca tatcccgtca ttcgtattta cgctgtcggt tcggcgaact ttatttcctg
     8041 cccggccttg atcgtcacga tcgctttctt ccaatcggaa cgatatccgc cgcgcgggcc
     8101 catccgacgc cgcatcttcc ccggcatgag agcggtattg acgcgcagga cgtccacctt
     8161 aaaataggcc tccacctctt ctttcacctg ccccttggtg gcgccggggt ccacttcaaa
     8221 aacgtatttg ttctgttcct tcatcgcggt ggaccgttcc gtcacgatgg gccttcgcaa
     8281 aacgctcatg gcttattcct tcccggcggc cgggcccgcg ggggcgaggt caccgttcag
     8341 ccgctgcact atcttatcca acgccggctt gctgaaaatc aattgttccg cctggagcgc
     8401 gtcgtaactg ttcaggttct ccacccggca gagacgcagg ccggccatat tgcgcgccgc
     8461 gcgcaggaac accggttcga tctgatccag gaccatcacc gtgcggcccg gggcgttcaa
     8521 ttttttcacc atggcggcca cgtgtttcgt cttcggttca ggcacggaga ccgcgtccac
     8581 cactttcatg cggccatcct tcaccaggtc cgttaaaatc atcttcaacg ctaagcgacg
     8641 cttcgcttcc gggaccgact gataataact gcgcggtttg ggaccgaacg tgacaccgcc
     8701 gtgtctccac agcggcgaac ggatggaacc cgaccgcgca cggccggtat gtttttgttt
     8761 ccagggtttc accccgccgc cggacacctc tccccgcgtt ttggtggagt gtgtcccgcg
     8821 acgacggttg gccaggtacg cgttgatgac ttcgtgcacc agggccggag atcccttaac
     8881 cccgaaaacc gcctccggca gatcgacgct ggagaccttt tcacctttca cgttcagaac
     8941 atccatgctc aaaggcatct tatttccgtc ccttcgctcg tccgtcaact atttcttcgc
     9001 gggcgccttg gcggccgccg gttgcttggc ggcttttttt gccttcggcg ccggaggaac
     9061 ctttttggct ttcatgggcc gggtcgtcgg gcgtatcgtc agacacgtgt ccaacgggcc
     9121 cggcaccgat cccttcaaca aaaggaggtt ccgatcggca tccacttttt cgacggtaag
     9181 ccgctgaacg gtcacccaat cgacgcccat atggccgggc ccccgtatcc ctttaaaaac
     9241 tctttgcggc cgctgcccgc cgctcgtccc cggagcgcgc catcggtccg actgaccgtg
     9301 cgtcttcgga ccgccgcgga accgatgccg tttcacgacg ccggcgaacc ccttcccctt
     9361 ggaatacccg gagacgtcca gcacgtcccc ctcggaaaaa ttcgtcaccc ggatgacttg
     9421 tccgggccga aaaccctcca ccttcgggag gcggatctcg cgcacccacc ggacgggctt
     9481 cacctgggcc ttggcgaact ggcccttcag cgccttattg acgtttttct ccttggcgtc
     9541 tccgaaaccg acctgcaacg cggcgtagcc gtcgctgtcg acggtcttga cctggagaat
     9601 gggacacggc cccgcctcca ccaccgtgac gggcacgaac tccccgttcg gtcgatagat
     9661 gcgcgtcatg ccgatcttcc gccccaaaat gcttttggtg ggcggggcct ccgccggggc
     9721 caccggattc gccggggcgg gctgggccgc cgggtcggcg ggaggaggcg tcgctgtgac
     9781 ttcggttttc tgttcttcca tggcctggtc ttagagttta atctccacgt ccacgccggc
     9841 cggcagatcc aaccgcatca tctc
//
LOCUS       DAOTFD010000070         9857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4860_length_9857_cov_4.390431, whole genome shotgun sequence.
ACCESSION   DAOTFD010000070 DAOTFD010000000
VERSION     DAOTFD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4860_length_9857_cov_4.390431"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="P5079_05790"
     CDS             complement(<1..633)
                     /locus_tag="P5079_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29536.1"
                     /translation="MNRTVLGGLGKWGRARIARRTDGEGVTLLILEPTLVCNSRCSAC
                     YNLNRLNPPPVVQITLAEIESVARALPHLAVLSLSGGEPTMHPCLSAVVEVFERHCRP
                     LAVNLPTNGVNPAQVAAAVEGVCGATRAQVVVHLSVDGIGEEHDRIRGVPGNFESLRD
                     TRARLRELQKHCPHLRLSVNTCLSARNRGSYRDILAWVEREWPEIEAHSVS"
     gene            complement(626..2323)
                     /locus_tag="P5079_05795"
     CDS             complement(626..2323)
                     /locus_tag="P5079_05795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29537.1"
                     /translation="MDGNSRRGGLPRMTGPVVLGGVLLLFLALASRMPSLPGPHYDEL
                     YNVSQATEFVLGLPLTTPWSVSFGGRDWPIAMRQPYDINTFIIYVGIGALKVFGVKVA
                     GLRITGVLTAALLLFLFYKLWRLWFSEPVALVSVVLAAASPAVLIWGRTAFFTDLFFS
                     LVFAHLSLLCASLWYKKGDVRMFYAACFLCAFELNISVRGLAHVVAYPVAFFLLVPRS
                     RWPRWGQILRGAGAGVLAAVVPLGLNAAAGWPSAVSLLGGLSSPTVGGVDNWNILNNV
                     AIRCGQFSAVLRGVWPTESIGGTAVPNPVILPVFLAAAAACLLDLFSKRSPPRPILKL
                     VLFLPAGMLFLTGFTPSNLGPIHLMAVWPYPALILGIVLGSLMSGEWTAGRPERWRRM
                     GKIGAAAVFAALLAFEGRVIGDYFRTLSKTGGDGYWSDSVYELARYLDAEKINQPICL
                     TWGIGMNVYFLTEGRVHPIEGHRCRLGGNLTLRDRYVRWVKEPGHHFVSITPDFESQG
                     VTYGYYKLFDDVLAGLGKRKVVVKTIKTRRGQDWFFVCRVEDEPPPARSRGTRGANE"
     gene            complement(2310..3968)
                     /locus_tag="P5079_05800"
     CDS             complement(2310..3968)
                     /locus_tag="P5079_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29538.1"
                     /translation="MTRMMKWGEVVFLLGTLLVFFAVAGRSLHLPGLYYDEVYNSRHA
                     MEFVSGLPLTTPWSVNFRGMDWPLASQQPYDINTVDVYAGILAMKIFGVSVAGIRTAG
                     VLTAGLLLVLFYALWRVWFSKTIAVVSVILAAMSPTILILSRFAFYEDLFFSLIFAHL
                     SLLCASLWYKKDDLRMFYAACFFCAFELNVSMRGLAYVASFPAAFFLFVPKARRPRFS
                     KILIGLGVGVLAALIPLALNAAAGGTSAAFLLGSLFSPTMDGVNNCSVLSNLAVRGVQ
                     FSEMLRGVGPTESIGGTAVPNPVILPVFLAAAAACLLDLFSKRSPPRPILKLVLFLPA
                     GMLFLTGFTPSNLGPIHLMAVWPYPALILGIVLGSLMSGEWTAGRPERWRRMGKIGAA
                     AVFAALLAFEGRVIGDYFRTLSKTGGDGYWSDSVYELARYLDAEKINQPICLTWGIGM
                     NVYFLTEGRVHPIEGHRCRLGGNLTLRDRYVRWVKEPGHHFVSITPDFESQGVTYGYY
                     KLFDDVLAGLGKRKVVVKTIKTRRGQDWFFVCRVEDGKDADGRK"
     gene            complement(3965..5425)
                     /locus_tag="P5079_05805"
     CDS             complement(3965..5425)
                     /locus_tag="P5079_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29539.1"
                     /translation="MKIFLVDPPFEIQDSASGNPSLKWVLNVVPSMGLGYLAAVAERA
                     GHEVRVVDGTLGLSDRELDRLVSDFSPDVVGFTSTTPAFRGARRLAKRVRELFPGRLI
                     VLGGAHVSALPEEALASGCFDVGVVGEGEHTFSELLESYGRDRDFDRRKIRGIVYRED
                     GVVKRTSPRPLMDDLDDLPFPARHLFPPLSKYSPTPASYRRLPVGIVMTSRGCPSRCA
                     FCDRSVFGETYRRRSPENVVAEVEALLRDFGAREIRFFDDTFTLSPHWIRRFISLLKE
                     RRLQFPWTCLSKVRGVSRELFEGMKEGGCWQVLFGLESGDPEMLRLLGKGNTVEDNGA
                     AVRAAHEAGLSVRGDFVIGAPGDSLEKMERTFRHAVDLKLEYAHFNKFVPYPGTELFR
                     QMRAAGKPVDFDAGSSSILDHDAIFHVPAGLDRESYRKWLNGVYGRYYLRPSYLFRRA
                     VALRSWDEFLGQVRGGVSILGLGVSRKMRSASLGNR"
     gene            complement(5508..6404)
                     /locus_tag="P5079_05810"
     CDS             complement(5508..6404)
                     /locus_tag="P5079_05810"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRY29540.1"
                     /translation="MKNRSARLRSRVRRIAAAWSVVALLVPAMRARASGDFVWRQESN
                     NVFGAGETLLYVVKYGLIPAGFATLEVRSVDTVDGRPAYYIMSRARTNKAMDLVFKVR
                     DKNESWMDVQSLCSLMASQQIREGLYKRTTITAYNHPRGRFTYVKRRKGKETITEGGI
                     PAFVQDVLSSLYYIRTRNLEVGKKYSFDANSGGKTWPLTVNVQEIEKVRVAAGKFNCF
                     HLEPILAGEGIFQQEGRLEVWVTADERKMPVLLRSRVMIGAFDAELVEFSTTGGSNII
                     KEKGLDEVEQEEEPAAAPIPKN"
     gene            complement(6421..6972)
                     /locus_tag="P5079_05815"
     CDS             complement(6421..6972)
                     /locus_tag="P5079_05815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF013394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY29541.1"
                     /translation="MGRWRVFMGFLAGAVYVYFSHVASWQRMAAGLTVALLGLLLRAW
                     AAGYLEKGKRLAQDGPYSYWRHPLYAGSFFMAAGFCLAGTGSGRTWHGFLLWLVFFAL
                     FFVVYPRRIREEEASLEKYFGDAWRAYVKRTMRFLPRLIPLKRENPDGFLLGRYRKNG
                     EYNAALGYLAGAGLLLLKLKLGF"
     gene            complement(7076..8164)
                     /locus_tag="P5079_05820"
     CDS             complement(7076..8164)
                     /locus_tag="P5079_05820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29542.1"
                     /translation="MPRIGIALRNFSLLTGVSRCVAELARRLPEQGYEPFLLTRRLPK
                     ESVGDLAVETVPWLPLSSWFRAASFDWFARRRLAARGVSLVHGHGDLTRQDVLSVHNC
                     DAAAAHYVPDGRRPAAGVEYVRKRQFTDEGSRVIVANSDMVRRDLSEFYGVRPEKIRR
                     VYLGVDLQRFHPDLRVSARAGLLKRARWPEETAVVVSVMSGDPAKRNFGLLSRAVSLL
                     AERRPAALCVVGNADWKTDDSARRLHEKGRLLAVPATLRVQDYFAAADVFMLPAFYEE
                     FGLTVLEALASGCPCAVSRRCGAAELISHGENGFVFDDVRNPEEAVHALEEALSPKRE
                     RVLCRRSVERYSWDNHAREIVELYRGLL"
     gene            complement(8173..9048)
                     /locus_tag="P5079_05825"
     CDS             complement(8173..9048)
                     /locus_tag="P5079_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY29543.1"
                     /translation="MDARHRLGYFLEYLLVRIIHGTLSALPRPAGSVLGRVLGRLIGG
                     LFPSRTRLAVSNMTQAFPDASPVQIRRWAAEGWESLGQAAWEFARFGASEKKYLEFVK
                     LEGVEHIRTSYRKGKGAILFTAHYTNWELTSGMVALSGCPVGVIARRMKNPYVNDFLT
                     RLRSRLQVRVFMHKQAVQESIRWLKKGNLLGVLFDQRITDGGVSVPFFGRPAHTTTLP
                     ALLALRLGCPLHPVHCWREDDRLRIHIGPPVDVSGLRPVEEDIRRLTERLNGVVEEWV
                     RRRPPMWLWIHNRWK"
     gene            complement(9015..>9857)
                     /gene="lpxK"
                     /locus_tag="P5079_05830"
     CDS             complement(9015..>9857)
                     /gene="lpxK"
                     /locus_tag="P5079_05830"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRY29544.1"
                     /translation="HPRQPFGDQQRFVAGEGDGKNTVRDAALAGDEPLLIAERLPGVP
                     VVVGADRARAARAAVADHSADCLVMDDGFQHLRLHRDRNLLCVDALSDPAVFSRPFGA
                     LLPAGPLREPPSGLRRADLVFLTKANLSSADQIERWRSFFRRQAPDVPVVPVRYELSF
                     YRRPSGELVAGDDIQGRAVLAFTGLARPESFEAALRRSSADVFCARFPDHHFFTAAER
                     EGVLQRAERENRRIITTEKDAQRLPPDFPCLVAKLDWRPDPADLERKGADFEWTRVIV
                     SAIS"
BASE COUNT         2205 a         3333 c         2754 g         1565 t
ORIGIN      
        1 ggaaacgctg tgcgcctcaa tctccggcca ttcccgctcg acccacgcga ggatgtcccg
       61 gtaggaaccg cggttccgcg ccgacaggca ggtgttgacg ctgaggcgca aatggggaca
      121 atgcttctga agttccctca aacgggcccg cgtgtctcgc aacgattcga aattccccgg
      181 cacgccccgg atccggtcgt gttcttctcc gatgccgtcc accgaaagat gcaccaccac
      241 ctgggcccgg gtggcgccgc acacgccctc cacggccgcg gcgacctggg ccggattaac
      301 gccgttggtc ggcaaattga cggccaaggg ccggcaatgg cgttcgaaca cctccaccac
      361 cgctgacagg cacggatgca tcgtcggttc tccgccgctc aacgagagga cggcgagatg
      421 aggcaacgcg cgcgccacgg actcgatctc cgccaaagtg atttggacga cgggcggagg
      481 gttgaggcgg ttcagattat aacaggcgga acaacgggaa ttgcaaacca gcgtcggctc
      541 caaaatcaac agggtgacac cttccccgtc ggttcggcga gcgattcttg cccgtcccca
      601 ctttcccaat cctcccagga ccgttctatt cattcgcgcc ccgcgtccct ctcgaccggg
      661 ccggcggcgg ctcgtcttcc acccgacaga caaaaaacca atcctgcccc cggcgggtct
      721 tgatggtctt cacgaccacc ttgcgcttcc ccagtcccgc caagacatca tcgaacaatt
      781 tatagtaccc atacgtgact ccttggctct cgaaatccgg cgttatggag acaaaatgat
      841 ggcccggctc tttaacccat ctcacataac gatcacgcag ggtcagattc ccccccaagc
      901 gacaccgatg cccttcgatc ggatggaccc gcccctccgt caaaaaataa acgttcatgc
      961 cgatccccca agtcaagcag atgggttgat ttattttttc ggcgtccaga taacgggcca
     1021 gctcatagac gctgtccgac caatacccgt cgccgcccgt cttcgacaac gtccggaaat
     1081 aatcgccgat cacgcgcccc tcgaacgcca ggagcgccgc gaacaccgcc gccgccccga
     1141 tcttccccat ccgccgccat cgttcgggcc gacccgccgt ccactccccg ctcatcagcg
     1201 accccaaaac gatccccagg atcaaggccg gatacggcca aaccgccata agatggatgg
     1261 gccccaggtt ggacggggtg aaaccggtga ggaacagcat ccccgccggc aaaaaaagaa
     1321 ccagtttcag gatcggcctc ggcggactcc tcttcgaaaa gaggtccagc agacacgccg
     1381 ccgcggccgc caggaaaacc ggcaggatga ccggattggg cacggccgtc cccccgatgg
     1441 attccgtcgg ccaaacaccc cgcagcaccg ccgaaaattg cccgcaccgg atcgccacgt
     1501 tgttcaaaat gttccaattg tcgacccctc ccaccgtcgg agaagacagc ccccccagca
     1561 acgaaacggc ggaaggccag cccgccgcgg cgttgagacc gagcgggacg acggccgcca
     1621 gaacgccggc ccccgcgccg cgcaggatct ggccccatcg gggccaacgg gaccggggga
     1681 ccaggaggaa gaaggccacg ggataagcca ccacatgggc caatccgcgc acggaaatgt
     1741 tcagttcgaa cgcgcagagg aaacaggccg catagaacat cctcacgtcg ccctttttat
     1801 accacagcga cgcgcacaac aggctcagat gcgcgaagac aagactgaaa aaaagatccg
     1861 taaaaaaagc cgttcggccc caaatcaaaa cggcggggct ggcggcggcc aaaaccacgg
     1921 agacgagggc caccggctcg gaaaaccaaa gacgccacaa cttatagaag aggaacagca
     1981 ggagcgccgc cgtcaacacc cccgtgatcc gcaaacccgc caccttgacc ccgaaaacct
     2041 tcagcgcccc gatcccgacg taaataatga acgtgttgat gtcatagggt tgccgcatgg
     2101 cgatcggcca gtcccgtccc ccgaacgaca cggaccaagg cgtggtcagc ggaagcccca
     2161 ggacaaattc cgtagcctgc gacacgttgt agagctcatc gtaatgcggg cccggcagag
     2221 aaggcatccg gctggccagc gccaggaaaa gaagcagcac gcctcccaac acgaccggcc
     2281 ccgtcatgcg cggcagaccg cccctccggc tatttccgtc catcggcatc cttcccgtct
     2341 tccacccgac agacaaaaaa ccaatcctgc ccccggcggg tcttgatggt cttcacgacc
     2401 accttgcgct tccccagtcc cgccaagaca tcatcgaaca atttatagta cccatacgtg
     2461 actccttggc tctcgaaatc cggcgttatg gagacaaaat gatggcccgg ctctttaacc
     2521 catctcacat aacgatcacg cagggtcaga ttccccccca agcgacaccg atgcccttcg
     2581 atcggatgga cccgcccctc cgtcaaaaaa taaacgttca tgccgatccc ccaagtcaag
     2641 cagatgggtt gatttatttt ttcggcgtcc agataacggg ccagctcata gacgctgtcc
     2701 gaccaatacc cgtcgccgcc cgtcttcgac aacgtccgga aataatcgcc gatcacgcgc
     2761 ccctcgaacg ccaggagcgc cgcgaacacc gccgccgccc cgatcttccc catccgccgc
     2821 catcgttcgg gccgacccgc cgtccactcc ccgctcatca gcgaccccaa aacgatcccc
     2881 aggatcaagg ccggatacgg ccaaaccgcc ataagatgga tgggccccag gttggacggg
     2941 gtgaaaccgg tgaggaacag catccccgcc ggcaaaaaaa gaaccagttt caggatcggc
     3001 ctcggcggac tcctcttcga aaagaggtcc agcagacacg ccgccgcggc cgccaggaaa
     3061 accggcagga tgaccggatt gggcacggcc gtccccccga tggattccgt cggcccgaca
     3121 ccccgcagca tttcagaaaa ttgcacgccc cgaaccgcga ggttgctcaa gacggagcaa
     3181 ttgttgacac cgtccatggt cggagagaac aaactcccca gcaaaaaagc ggcggacgtt
     3241 cccccggccg ccgcattgag cgcgaggggg atgagggccg ccagaacgcc gactcccaga
     3301 ccgatgagga ttttggaaaa cctcggtcgg cgggctttcg gcacaaaaag aaaaaaagcc
     3361 gccggaaaag acgccacgta ggccagcccc cgcatcgaga cattcagttc gaatgcgcaa
     3421 aagaagcagg ccgcataaaa cattcgcagg tcgtcctttt tataccacag cgacgcgcac
     3481 aacaggctca gatgcgcgaa gatgaggctg aagaacaaat cctcatagaa cgcgaaacgg
     3541 ctcaatatta aaattgtcgg actcatggcc gccaaaatga cggaaacaac ggcgatggtt
     3601 ttggagaacc agacgcgcca caacgcgtag aaaaggacca gcaggaggcc cgccgtcaac
     3661 accccggccg tccgaatacc ggccacgctc acgccaaaaa tcttcatcgc gaggatcccg
     3721 gcatagacat ccacggtatt gatgtcgtac ggctgttgcg aggcgagcgg ccaatccatt
     3781 ccccggaaat tgacggacca cggggtagtc aatgggagcc cggaaacgaa ttccatggca
     3841 tgtctcgaat tataaacctc gtcgtaatag agccccggga gatgaaggga acgtccggcc
     3901 acggcgaaaa acacgagaag cgtgccgagc aaaaaaacga cctcccccca cttcatcatg
     3961 cgcgtcatcg attccccagc gaagcggacc tcatcttacg cgaaactccc agcccaagaa
     4021 ttgaaacgcc gccacggacc tgaccgagaa actcatccca acttcgaagg gccaccgccc
     4081 tccgaaacaa ataggagggt cgcaggtaat acctcccgta aaccccgttg agccatttcc
     4141 gatacgattc ccgatccaaa ccggccggca catgaaagat ggcgtcgtga tccaagatac
     4201 tgctgctccc cgcgtcgaaa tcaaccggtt tcccggccgc ccgcatctgc cgaaaaagct
     4261 ccgtccctgg atacgggacg aacttattga aatgggcata ctccagtttc aaatcgaccg
     4321 cgtggcggaa cgtccgctcc atcttttcca aactgtcccc gggggctccg atcacgaagt
     4381 ccccccgcac gctgagcccg gcctcgtgcg ccgcccgaac cgccgcccca ttgtcctcga
     4441 cggtattccc cttccccagg agacgcagca tttccggatc cccgctttcc aagccgaaca
     4501 gaacctgcca gcaaccgccc tccttcattc cctcgaacag ttcccgggag acgcctctga
     4561 ctttgctcaa acaggtccat gggaactgca gccggcgttc tttcagaagc gaaatgaagc
     4621 ggcggatcca gtgagggctc aacgtgaacg tgtcgtcaaa aaaacggatt tcccgggcgc
     4681 cgaaatcgcg aagaagcgcc tccacctccg ccacgacgtt ttcagggctc cggcgacgat
     4741 aagtttcccc gaacaccgac cggtcgcaga aagcgcaccg ggaggggcat ccccgactcg
     4801 tcatcacgat ccccaccggc aaccggcgat aggacgccgg ggtcggcgaa tatttcgaca
     4861 gcggcggaaa aagatgccgc gccgggaacg gcaaatcgtc gaggtcgtcc atcaacgggc
     4921 ggggcgacgt cctttttaca acgccgtcct cccggtagac aatgccgcgt attttccgtc
     4981 ggtcaaagtc ccgatcccgc ccgtaggatt caaggagttc gctgaaggta tgctcccctt
     5041 cccccacgac gccgacatcg aaacaaccgg acgcaagcgc ctcttccgga agagcgctca
     5101 cgtgcgctcc gcccaggacg atcaaccggc ccggaaagag ctcccgaacc cgttttgcca
     5161 gccgccgagc cccccggaaa gcgggggtcg tggaggtgaa cccgacaacg tccggcgaaa
     5221 aatcggaaac gagccggtcc agctcccgat ccgaaaggcc gagcgtgcca tcaacgaccc
     5281 gcacctcatg gccggcccgc tccgccaccg cggcaagata tcccagcccc atagacggga
     5341 cgacgttcaa cacccatttc aagctggggt taccggaggc ggaatcctga atttcaaaag
     5401 gcgggtccac taaaaatatt ttcatgggtg ggaattcctg tgatcgaaaa accttttcaa
     5461 gcaaacgtgc cgacggcgaa acagcgacgg ttcccggcgg ggatgcgtca atttttcggg
     5521 atcggcgcgg cggccggctc ctcttcctgt tcgacctcgt ccagcccctt ctccttgatg
     5581 atgttcgaac cgcctgtcgt tgaaaattcc acgagttcgg cgtcgaacgc gccgatcatg
     5641 acgcgggacc gcaggagcac cggcattttc cgctcgtccg ccgtcaccca gacctccagc
     5701 cgcccctcct gctggaaaat gccttcaccg gcgaggatcg gctccaaatg gaaacagtta
     5761 aatttcccgg ccgccacccg caccttttcg atctcctgca cgttcaccgt caaaggccac
     5821 gtctttccgc cggaattggc gtcgaaactg tatttttttc cgacctccag gttcctcgtg
     5881 cggatgtaat acaggctcga caggacgtcc tgcacaaacg ccgggatgcc gccctcggtg
     5941 atcgtttcct ttcccttccg ccgcttgacg taggtgaagc ggccccgggg atggttgtag
     6001 gccgtgatgg tggtgcgttt gtaaagtcct tcccggatct gttgcgaagc catcagcgaa
     6061 cacaggctct ggacgtccat ccaggactcg ttcttgtcac gcaccttgaa cacaaggtcc
     6121 atcgccttgt tggtgcgcgc ccgggacatg atgtaatagg ccggacgacc gtcgacggtg
     6181 tccaccgacc gcacctcgag cgtcgcgaac ccggccggga tcaatccgta tttcacgaca
     6241 tacaataagg tctcccccgc cccgaacacg ttgttggatt cctgccgcca cacgaaatcg
     6301 ccggaagccc tggccctcat ggccgggacg agcagcgcga cgaccgacca cgcggcggcg
     6361 attcgccgaa cgcgggaccg aagacgggcg gatcgatttt tcacgtcgtg gggagccccc
     6421 ctaaaaccca agtttcaatt tcaaaaggag gagtcccgcc ccggcgagat atcccagagc
     6481 cgcgttgtat tccccatttt tccgataacg ccccaaaaga aaaccatccg ggttttccct
     6541 tttcaacggg atgagccggg gcaaaaaccg catggtcctt ttgacgtagg cgcgccaggc
     6601 gtcgccgaaa tatttttcga gggaagcctc ctcttcgcgg atgcgccgcg gatagacgac
     6661 aaaaaacaac gcgaagaaaa ccagccacaa gaggaaaccg tgccatgtcc ggccggaacc
     6721 ggtcccggcc aggcaaaacc ccgccgccat gaagaaactg cccgcgtaaa gcgggtgccg
     6781 ccaataagaa tacggaccgt cctgcgccag ccgttttccc ttttccagat agccggcggc
     6841 ccacgcccgg agcaaaagcc ccaggagcgc gaccgtcaag ccggccgcca tccgctgcca
     6901 agacgccacg tgggaaaaat aaacgtacac ggctccggcc aaaaatccca taaacacgcg
     6961 ccaacgtccc attttcacat cctccttaaa cccattttcg aaatttttcg gaggcggcca
     7021 ttcccggccc gccggcccga cgacattcta ttataaataa gagggccgct ccatctcaca
     7081 ggaggcctcg atagagctcg acgatctccc gcgcatggtt gtcccaggaa tatctctcca
     7141 cggaacgacg gcagaggacc ctctccctct tcggcgagag cgcctcttcc agagcatgga
     7201 ccgcctcctc cgggttccga acgtcatcga acacgaaccc gttctccccg tgcgaaatca
     7261 gctccgccgc cccgcaccgg cggctcaccg cgcagggaca gccgctcgcc aatgcctcca
     7321 aaacggtcag gccgaactcc tcataaaaag caggcagcat gaaaacgtcc gcggcggcga
     7381 aataatcctg aaccctcaag gtcgccggga cggccagcag ccggcctttt tcatggaggc
     7441 ggcgcgccga atcgtccgtc ttccagtcgg cgttccccac cacgcacaag gccgccggcc
     7501 gccgttcggc caagagggac accgcccggg agagcagccc gaaattcctt ttcgcgggat
     7561 cgcccgacat caccgacacg acgacggcgg tctcctccgg ccagcgcgcc cgcttcagca
     7621 gccccgcccg cgccgagacg cgcaaatcgg gatgaaaccg ttgcaggtcc acgcccagat
     7681 agacgcgccg gattttttcc ggccggacgc cgtagaattc cgacaggtcc cggcggacca
     7741 tgtcggagtt ggcgacgatg acgcgactgc cctcgtcggt gaactgtctt ttccggacgt
     7801 attccacacc cgccgccgga cggcggccgt ccggcacgta atgcgccgcg gcggcatcgc
     7861 agttgtggac ggagagaacg tcctgccggg tcagatcgcc gtgcccgtgc accagagaga
     7921 ccccgcgggc ggcgagacgg cggcgcgcga accagtcgaa agaagcggca cggaaccaag
     7981 acgacagggg cagccagggg acggtctcga ccgccagatc cccgacggat tctttcggca
     8041 gacgccgcgt caagaggaac ggttcatagc cctgttccgg caggcggcgg gccagttccg
     8101 ccacgcaccg gctcacgccg gtgagtaacg agaaatttcg gagagcgatc ccgatgcgtg
     8161 gcatcaagcg ggttatttcc agcggttatg tatccagagc cacatgggag gacggcggcg
     8221 gacccattct tccaccacgc cgttcaggcg ttcggtcaaa cgccggatgt cctcctccac
     8281 gggccgcagt ccgctcacat ccacgggcgg gccgatgtgg atgcgcaagc ggtcgtcctc
     8341 ccgccaacag tggaccggat ggagcggaca tcccaaccgc agggccagga gcgccggcag
     8401 ggtggtggta tgcgccgggc ggccgaaaaa aggaacggag acgccgccgt cggtgatgcg
     8461 ctggtcgaag agcaccccca acaggttccc cttcttcaac cagcggatgg attcctgaac
     8521 ggcctgtttg tgcatgaaga ccctcacctg cagccgggaa cggagacggg tcaggaaatc
     8581 gttcacgtag ggattcttca tccgccgggc gatgacgccc acgggacagc cggagagggc
     8641 caccatgccg gaggtcaact cccaattggt gtaatgcgcg gtaaagagga tcgccccttt
     8701 gcctttccgg taggacgtcc ggatgtgttc aaccccctcc agcttgacga attccagata
     8761 ttttttttcc gaggccccga aacgggcgaa ctcccaggcg gcctgcccga gactttccca
     8821 gccctccgcg gcccatcgac ggatctggac cggtgaggcg tccgggaagg cctgggtcat
     8881 atttgaaacg gccaaccggg tacgggacgg aaagaggccg ccgatgagcc ggccgagaac
     8941 ccttcccaac acgcttccgg ccgggcgggg caaggccgac aacgttccat ggatgatccg
     9001 gaccagaagg tactctagga aatagccgag acgatgacgc gcgtccattc gaaatccgct
     9061 cctttccgtt ccaaatccgc cgggtcgggc cgccagtcca gtttcgccac gaggcaaggg
     9121 aaatccggcg gcaggcgctg ggcgtccttt tccgtggtga tgatgcgacg gttttcccgt
     9181 tcggcccgtt gaaggacacc ttccctttcg gcggccgtga agaagtggtg atcggggaaa
     9241 cgggcgcaaa acacgtcggc ggaggaccgc cgcaacgcgg cctcgaacga ttccggccgg
     9301 gccagtccgg tgaacgccag gacggcccgc ccttggatgt catcgccggc gacaagttct
     9361 cccgaaggcc gccggtaaaa cgaaagttcg tagcggaccg gcaccacggg gacgtcgggc
     9421 gcttgccgcc gaaagaaaga acgccagcgt tcgatttgat cggccgaaga caggttggct
     9481 ttcgtcaaaa agaccaggtc cgcccggcgc aggccggagg ggggttcgcg caacggtccc
     9541 gccggcagga gggcgccgaa cgggcgtgaa aaaaccgccg ggtccgaaag agcgtccacg
     9601 cacaacagat tccggtcccg atggagccgg agatgttgaa aaccgtcgtc catcacaaga
     9661 caatccgccg aatggtccgc caccgcggcg cgggccgcgc gggcccggtc cgccccgacg
     9721 accacgggca cccccggcag ccgttcggcg atcaacagcg gttcgtcgcc ggcgagggcg
     9781 gcgtccctca ccgtgttttt accgtcgccc tcgccggcga cgaaccgctg ttgatcgccg
     9841 aacggctgcc gggggtg
//
LOCUS       DAOTFD010000071         9854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4862_length_9854_cov_4.248801, whole genome shotgun sequence.
ACCESSION   DAOTFD010000071 DAOTFD010000000
VERSION     DAOTFD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4862_length_9854_cov_4.248801"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>9854
                     /locus_tag="P5079_05835"
     CDS             <1..>9854
                     /locus_tag="P5079_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRY29545.1"
                     /translation="TSIRAAFVEGYLDPETREEYAEALAWLKQVHPTLEDQLTFGGEL
                     LEKIHLLEGLGLTPQQIAATEFKQAEIKAVVRQKAEGRISKAVKAALDELEKSNFALW
                     LKLTNNGKYLGREISEDKVKKALGDTKVRRAARVASRVAESLSAGLEAAAGLSGAAEV
                     VKRVNRRTPKKKAAEPKKETAPRKVKTAETPAPVVSTPVKPEQTAETPAMDVSAPVEV
                     VAEQEPAAQPRKRTKAAKTVKAPTAPTITSPGTAAEPALSPINRSVFMAVSQMDDGIQ
                     VIAHDQVLKDLEARVQARLKELPKNQSPGTVKTVLKLFYAVALLHEVLASAGLPHEQI
                     VQAGFDLENFTPQKGVTVKSLIDAAKTLLSPETAAPEETVPSETATEETPAEAEALVE
                     AQPEQAAPAQIKTKSFASHKRAGNEVTVMVHKDVKMDAAARTRLILHEVLASAGLSHK
                     AIDAAGFSADGNAATQGKDSQELIALAKTLMTQAGKKAADVSFFGDEFIENVWAPVLN
                     ALFLLKGSKARTWKSFLSSLKKLGFANADKLDKARSVNAFLKLLSFDTLPLDVTQLVL
                     KRVGFNLAEIEDFVELKILQEIEQELGVKVKVQVVKGKPAAAKPVKAEKPAVVKKEAP
                     AAAKPAEDPWAAMAVERIPTVDEMYAAKMARKTAEAVFAFLQAQQGPVTLAHVRDAQL
                     GVGPKTLAKLQPLVKKAEAPKAVEKKPEAKPKKVAPKKTPTAAPSIDDQIAKLDAQIT
                     DKESELAELRDRQETLEETLQELQDRRWEMEKAAEGGSAFTPLFKKIDVVRGAPEMPV
                     MVTKDGTMLVPEFLMEKWREQEKLAPKDADGKPAKGYSLKERLVRELLQQDFKTRLAL
                     KLQELGPTATPAEREKAAQGILQNHPVQQISPVLLGVVPAQAASNEDEALKMARVILR
                     DFYKYGSSLAGVQILAPHQEQVELIRATLKKTLTRPAVKKALQETLEEQKAKKLAKEL
                     EVPGGKAGADPRPKMTSGGAKGAYTKLLNTIAAIETMLKSVDSDPNLDDMGKIAKNVA
                     VTFADYLGRREAAELLMISLVNDFADPLRALAGKRIRVGYYARAETTPDDQKGYFRYT
                     FTVSDRQTMHRSQAGRTAVSLRVEEELHISPIEGELRREETLTDVPRTLYPEWFDWSN
                     LEVEENGVWKRVQLEALPGTNARALKEYKGLRTILELQGRGIWFNTRIFGNHSLLMRM
                     EVANLLVGQPVLGKVHDLLSLGREWFYDPKLLRAVDWTAKGNTAVFKALAAALGVKAD
                     TVEDLAEKIGEALVPVILPQDLYEKLAALIPIETERGELKSKKAPEKKFEEALAEYGK
                     TGAKTTAPAASKDDQKKLADAVRAKEQESDDLQAELAEAQAELDSLKSQRQQLVSARK
                     KDVSAFGPAIFEQQVWLPILGALSQILKANDEVRRSLVESLRRLNLNAAADALDKDLA
                     PEALNQALRFDQLPFEIVRTVLDIVGYEILSLGAAEKTLEDVRKQIERALGMKIAVGL
                     VRTKQFAAETPTAKTESVELVAPAVEEKTPKTEETTPTAPETLPAADAQPLNINDATV
                     EQLAAVLKQTRAVVAYNKVAERIYAHVREKGALTSAQDLIDAHLGIGQKALEKLAQAI
                     RFGSATTPEQPVEPVEEVPETAQPPAEEPAAEETVIGPLGDLIDKLTGRTPTPFATPE
                     SLKGREKKIYEAVWSVLPEEFVSSPALMDSIVPLTQLIGALLKPEYPKLSQVELNAIV
                     AKMVEMGARKAEPPAVMSAEEAKKIMRTAVLRLAMNIQMRLNTVKDAAQKDLASQKIS
                     RGLRGYMEKYYSQLLLQDSQAENPVEKILARMQADTASKGMPELLRDAIRQLHDEWAA
                     AAKFGMEQLQGVNPKTKPSLFQLIGIRRLIANRRTLLADVMGTGKTLQVLLSIMNSPD
                     IQDTLIVAPLGGIETWVDEIIKRLKGPRDIVVLASDYQRIPSADDYTVTHHSGEEAAK
                     FVDQWIAELESGKTTGRKKFVMINYDQLKILEERRPGILERLKPHGLVIDEAHNIKNI
                     KETVIRTQLIQKIQSSYLALVTGTPIENVAQDAEIYLTMIFRDPQIEARVFADSSFEN
                     FREFWAAFRKRSTQPAMLAELNRIMNAVMVRRESEDVLIGLPAKVETNYILNLENATL
                     EQWDGDRLVETSPLAAGKEAFDVQVHLIQWLMGHTWTKTGRRLNTEDPALDLSKIWTP
                     EEFQKWNAFFEQQLATNKKFAAALPKDNAISLLTRVNQAFNDPELMGLKADSIKFAAL
                     KAIIEAKLKKGEQVIIYSNYTTITKKIKALFSPKLVSYVDGSVPMTKRFDQIRRFQNG
                     DSRLFLATVDAASEAIDLSRASAVLFFDKPWKPSTVSQGATRPYRWGNKNATLEFGTL
                     AVRWGQTDDSASTTLLRALGRPAKGTDAYVEQILHEKRLEERHTLQSGLPIEWPHRPD
                     AAAASAERVAARSISPQGQAGAPAMTLEDLRQKVEIVRIDKTMDWKKAKLEVDAVRRS
                     HVGREFLVNSIKANFVGRWNISLILRDKENGDIIGYVVGVPRGKRKELQTLYPDMDSR
                     TTLFVEEAVHTRMPPEIRELAPQLLDEELAKLAQSLGYKQIVRISKEAAESSPLVTSA
                     PEQVSTAVQTQALIEDFWATFKEVMSLAAVNAMVRREAEVSLRRIGYGNLADLLSAGE
                     KTVEELQSLVDISQLSQEVLFRVKEIAGFKRILRVEEGSEAIEDKLVREQINPYFYEN
                     GRSYLSEGKNFDALRSFMATLELRPAETTLWLALADTLYALGLKRSLKTASKLAFGEN
                     PLPATPEELRGIFDGLLAELGEKYPTEPAVDLLGYAAGPAFDELSQREDLTAEETAAL
                     VEVTQLRQGKKDIQQIISYNELVTIKEIPVDVALRVYSYINARRRVGKPVTSLGALID
                     LEGMTAPRLLKLMELFYVPGAELEDAMWRVRYEKDQAKAKYAEAEGAENLQRRQAFEA
                     AKGELRRLLALNGVVSAVQNRSLRERRGALGRAFDHLFGVLVKADPVVNTVKAALGLA
                     AEKKQPLVLLPEETAERFAELQDELAPLTDHYRTLRETYVLKNNYRLSTADLRALTAE
                     LEKPVPLTLRDGTTVERSLIDRLEDLAKAYPEISLELMEMLDVVLFYAYQQGTTPSPD
                     AVSKLTEFSRAVMDYQKKAAPAKAAPELPEFHKESKPANTSYSDALIADQEEVGLVKL
                     SKAMEKEWGQVKLLIIDRLAGKNVPKDILAMLKTAKFKREAKEPKPWSAVVGGRIAWA
                     GAYVTEDRRLYIRGHFLQALSKEELADLIANTLLASMEETAAPAQQPAAT"
BASE COUNT         2322 a         3157 c         2960 g         1415 t
ORIGIN      
        1 accagcatcc gggccgcgtt cgtggaggga tatctcgacc ccgaaacgcg cgaggaatac
       61 gccgaagccc tcgcctggct gaaacaagtc caccccacac tggaagacca gctcacgttc
      121 ggcggtgaac tcttggaaaa aatccacctc ctcgaagggc tggggctgac accgcaacag
      181 attgcggcaa cggagttcaa acaggcggaa atcaaggccg tcgtgcggca aaaagccgag
      241 gggagaatct cgaaagcggt caaggccgcg ctggacgaac tggagaaatc caatttcgcg
      301 ctctggctca agctcaccaa caacggcaaa tatctgggcc gggagatttc ggaagacaag
      361 gtgaaaaaag ccctcggcga caccaaagtc cgccgggcgg cccgcgtcgc ctcccgcgtc
      421 gccgaatccc tgagcgccgg tctcgaagcg gcggccgggc tgagcggcgc cgcggaggtg
      481 gtgaaacgcg tcaaccgccg gaccccgaag aaaaaagcgg cggaaccaaa aaaggaaacg
      541 gctccccgaa aagtcaaaac cgcggaaacg cccgcgcccg tcgtttcgac ccctgtgaaa
      601 ccggaacaaa cggcggaaac gcccgcgatg gacgtctcgg cgcccgtcga ggtggtggcc
      661 gaacaggaac ccgcggcgca gccgaggaag cggaccaagg ccgccaaaac cgtcaaggcc
      721 ccaaccgccc cgacaataac ctcgccggga acggccgcgg aaccggcgct ctcgccgatc
      781 aatcggagcg ttttcatggc ggtctcccaa atggacgacg gcatccaggt catcgcccat
      841 gaccaggtcc tgaaggattt ggaggcgcgc gtgcaagctc ggctgaagga actacccaag
      901 aaccaatctc ccggcaccgt caaaaccgtt ctaaaactct tctacgccgt cgccctcctg
      961 cacgaggtcc ttgcctccgc cggcctcccg cacgaacaga tcgtccaagc cggtttcgac
     1021 ttggaaaatt tcactcccca aaaaggcgtc accgtcaaat cgttgatcga cgcggccaaa
     1081 acccttcttt cgcccgaaac agcggccccc gaagaaaccg ttccatccga aacagcaacg
     1141 gaagaaaccc ccgctgaggc cgaagccctc gtcgaagccc agccagaaca ggcggcgccg
     1201 gctcagataa agacgaagtc tttcgccagc cacaaacggg ccggcaacga agtcacggtg
     1261 atggtgcaca aggacgtgaa gatggacgcc gccgcccgga cgcggctgat ccttcacgag
     1321 gtgctggcct cggcgggcct gtcgcacaaa gccatcgatg cggccggatt ctccgccgac
     1381 ggcaacgcgg ccacccaggg gaaggactcc caggaattga tcgccctggc caaaacgctg
     1441 atgacccaag ccggcaaaaa agccgccgac gtctccttct tcggcgacga gttcatcgag
     1501 aacgtctggg cgccggtcct gaacgccctc ttcctattga agggatccaa agcccggacc
     1561 tggaaatcct tcctctcctc cctgaaaaaa ctcggttttg caaacgcgga caaactggac
     1621 aaggcgcgtt ccgtgaacgc tttcctcaaa ctcctctcgt tcgacacgct gccgttggac
     1681 gtcacgcaat tggtcctgaa acgggtgggg ttcaacctgg cggagatcga ggatttcgtc
     1741 gaactgaaga tcctccagga aatcgagcag gaactgggcg tgaaggtgaa agtccaggtc
     1801 gtcaagggga aacccgccgc ggcgaaaccc gtgaaagccg aaaaaccggc ggtggtgaaa
     1861 aaagaagctc ccgcggcggc gaaaccggcc gaggatccgt gggcggcgat ggcggttgaa
     1921 cgtatcccca ccgtggacga gatgtatgcc gccaagatgg cccgcaaaac cgcggaggcg
     1981 gtgttcgcgt tcctgcaggc tcaacaggga ccggtcaccc tggcccacgt gcgagacgcg
     2041 caactgggcg tgggccccaa gaccctcgcc aaactccagc ccctggtgaa aaaggccgaa
     2101 gcgccgaaag ccgttgaaaa gaaaccggaa gccaaaccga aaaaagtcgc gcccaagaaa
     2161 accccgacgg ccgcacccag catcgacgac cagatcgcca aactcgacgc gcagatcacc
     2221 gacaaagagt cggaactcgc cgaactccgc gaccggcagg aaacccttga ggaaaccctt
     2281 caggaactgc aggaccgccg ctgggagatg gaaaaagccg cggaaggcgg gagcgccttc
     2341 actccgctgt tcaagaagat cgatgtggtg cgcggagcgc cggagatgcc ggtgatggtg
     2401 acgaaagacg gcacgatgct ggtgccggaa ttcctcatgg agaaatggcg cgaacaggaa
     2461 aaactggcgc ccaaggatgc ggatgggaaa ccggcgaaag gctattctct gaaggagcgc
     2521 ctggtccggg aacttcttca acaagatttc aaaacgcgtc tggccctgaa actgcaggaa
     2581 ctggggccga ccgccacgcc ggcggaacgc gaaaaagccg ctcaaggaat cctgcaaaat
     2641 cacccggttc aacaaataag ccccgtcctg ttgggggttg tccccgctca ggctgcctcc
     2701 aacgaggacg aggcgctcaa gatggcccgg gtgatcttgc gggatttcta caagtacggc
     2761 agttcgctgg ccggcgtcca gatactggcg ccgcaccagg aacaggtgga actgatccgc
     2821 gccacgctga agaagaccct gacccggccg gcggtcaaga aagcgctcca ggaaacgctg
     2881 gaggagcaaa aggcaaagaa actcgccaag gaactggaag tccccggcgg caaggcgggg
     2941 gcggatccgc gtcccaaaat gacgagcggg ggggccaaag gggcctacac caaactgctc
     3001 aacaccatcg cggccatcga gacgatgctg aagtccgtgg acagcgaccc gaacctggac
     3061 gacatgggga agatcgcgaa gaacgtggcg gtcaccttcg ccgactacct cggccggcgc
     3121 gaagcggcgg aactgctgat gatttcgctg gtgaacgatt tcgccgaccc gctgcgggcg
     3181 ctggccggca aacgcatccg cgtcggctac tacgcccgtg cggaaaccac cccggacgat
     3241 cagaaagggt atttccgcta tacgttcacg gtctcggacc gtcagaccat gcaccgcagc
     3301 caggccggcc gcacggcggt gagtctgcgg gtggaggaag aactgcacat ctcgcccatc
     3361 gaaggcgagc tgcgccggga agagacgtta acagatgtcc cccgcacgct gtatccggaa
     3421 tggttcgact ggagcaacct ggaagtggag gagaacggcg tctggaaacg ggtgcagttg
     3481 gaggccctcc ccggcacgaa cgcccgcgcg ttaaaggaat acaaaggact gcggaccatc
     3541 ctggaactgc aaggccgcgg catctggttc aatacgcgga ttttcggaaa ccacagcctg
     3601 ctgatgcgga tggaagtggc caacctcctg gtcggtcagc cggtgctggg caaagtgcac
     3661 gacctgctct cgctgggccg ggaatggttc tacgacccga aactgctgcg ggcggtggac
     3721 tggacggcca aaggcaacac ggcagtgttc aaggccctgg cggcggcctt gggcgtgaaa
     3781 gccgacacgg tggaagacct ggcggaaaaa atcggagagg cgctggtccc ggtgatcttg
     3841 ccgcaggacc tctacgaaaa actggccgct ctgatcccca tcgagacgga gcggggggaa
     3901 ttgaagagca agaaagcccc cgagaaaaaa ttcgaagaag ccctggcgga atacggcaag
     3961 accggcgcga aaacgaccgc cccggcggcc tccaaggatg accagaaaaa actggcggac
     4021 gccgtccgcg ctaaggagca ggaaagcgac gacctgcagg ccgaactcgc cgaagcccag
     4081 gcggagctgg attccctgaa atcccagcgg caacaactgg tctcggcgcg gaaaaaagac
     4141 gtctcggctt ttggtccggc catcttcgaa cagcaggtat ggctgccgat cctgggggcg
     4201 ctgtctcaga ttttgaaagc caacgacgag gtgcggcgat ccctcgtgga atccctgcgc
     4261 cgactcaacc tgaacgccgc ggccgacgcg ctggacaagg acttggcgcc ggaagccctc
     4321 aaccaggcgc tgcggttcga ccaactgccg ttcgaaatcg tccggacggt gctggacatc
     4381 gtcggttacg aaatcctctc gctcggcgcc gcggaaaaaa cgctggaaga tgtacgcaaa
     4441 cagatcgagc gcgctctggg catgaagata gccgtggggc tggtgcggac aaaacaattc
     4501 gccgccgaga cccccacggc gaaaaccgag tccgtcgaac tcgtcgcgcc ggccgttgaa
     4561 gagaaaaccc cgaaaaccga agaaacgacg ccgacggcgc cggaaacgct cccggccgcc
     4621 gacgcccagc cgctcaacat caacgacgcc actgtcgaac agctggccgc ggtgctgaaa
     4681 cagacgcgcg ccgtggtcgc ctacaacaaa gtggccgaac ggatttacgc ccacgtccgg
     4741 gaaaagggcg ccctcaccag cgcccaggac ctgatcgacg cccacctcgg catcggccaa
     4801 aaagccctgg agaaattggc ccaggcgatc cgctttggtt ccgcaacgac accggagcag
     4861 ccggtcgaac ccgtcgaaga agtcccggaa accgcccagc cgccggcgga ggaacccgcc
     4921 gccgaggaaa ccgtcatcgg tcccctgggc gacctgatcg acaaactcac gggccggacg
     4981 cccacgccct tcgccacgcc ggaaagcctg aaaggtcgcg agaagaagat ctacgaggcc
     5041 gtgtggagcg ttctgccgga agaattcgtc tcctctccgg ccctgatgga ttccatcgtt
     5101 ccgttgactc aactcatcgg cgctctgctc aaaccggaat atccgaaact ctcccaggtc
     5161 gaactgaacg ccattgtggc caagatggtc gaaatgggcg cccgaaaagc cgagccgccg
     5221 gcggtgatga gcgccgagga ggcgaaaaaa atcatgcgga ccgccgttct gcggctggcc
     5281 atgaacatcc agatgcgcct gaacacggtc aaagatgctg cccaaaaaga cctggcctcc
     5341 caaaaaatca gccgcggact ccgcgggtac atggagaaat actattcaca gctgctcctg
     5401 caggattccc aggccgagaa ccccgttgaa aaaatcctcg cccggatgca ggccgacacc
     5461 gcctccaaag gcatgccgga actcctccgc gacgccatcc gccagctcca cgacgaatgg
     5521 gccgccgcgg ccaagttcgg catggaacag ctccagggag tgaaccccaa aaccaaaccc
     5581 agcctgttcc agctgatcgg catccgacgg ctcatcgcca accgccgcac cctcctggcc
     5641 gacgtgatgg gcacaggaaa gaccctgcag gtcttgctct ccatcatgaa cagcccggac
     5701 atccaggaca ctctcatcgt ggcgccgctg ggcggcatcg agacctgggt ggacgaaatc
     5761 atcaaacggc tcaagggccc gcgggacatc gtggtactgg cctccgatta tcagcgcatc
     5821 cccagcgccg acgactacac cgtcacccac cacagcggcg aggaggcggc gaagttcgtg
     5881 gatcaatgga tcgcggaact ggagtccggc aagaccaccg gccgcaaaaa gttcgtgatg
     5941 attaattacg accaactgaa gattttggag gagcgccggc ccggcatcct cgaacggctc
     6001 aaaccgcacg gcctggtgat cgacgaggcc cacaacatca agaacatcaa ggagacggtg
     6061 atccgcaccc agctgatcca gaagatccag tcgtcctacc tggcgctggt gacgggcacg
     6121 cccatcgaga acgtggccca ggacgcggag atctacctga ccatgatctt ccgtgacccg
     6181 cagatcgagg cgcgcgtgtt cgcggattca tccttcgaga atttccgcga gttctgggcg
     6241 gcgttccgca aacggtcgac ccagccggcc atgctggcgg aactgaaccg catcatgaac
     6301 gccgtgatgg tgcggcgcga gtcggaagac gtgctgatcg gcctgcccgc caaagtggag
     6361 acgaactaca ttctcaacct cgaaaacgcc acgctggaac aatgggacgg ggaccgtctg
     6421 gtggagacct ccccgctggc tgccggcaag gaggcgttcg acgtccaggt ccatctcatc
     6481 cagtggttga tggggcacac atggacgaag accggccgcc ggctgaacac ggaggacccc
     6541 gcgctggacc tctcgaagat ttggacgccc gaggagttcc agaaatggaa cgccttcttc
     6601 gaacagcagt tggccacgaa caagaagttc gccgccgccc tgcccaagga caacgccatt
     6661 tcgctgttga cccgcgtcaa ccaggctttc aacgatccgg aactgatggg gctcaaggcg
     6721 gactccatca agttcgccgc cttgaaagcc atcatcgaag ccaaactgaa gaagggcgaa
     6781 caagtcatca tctactccaa ctacaccacc atcaccaaga agatcaaagc gctgttcagc
     6841 cccaagttgg tgtcctacgt cgacggctcg gtgccgatga cgaaacggtt cgaccaaatc
     6901 cgccggttcc aaaacggcga ctcccgcctg ttcctggcca cggtggacgc cgcctccgaa
     6961 gccatcgacc tgagccgcgc cagcgccgtc ctcttcttcg acaaaccctg gaaaccctcc
     7021 acggtgagcc agggagccac gcggccctat cggtggggga acaagaacgc cacgctggaa
     7081 ttcggcacgt tggccgtccg ctggggacag accgacgatt ccgcgtcgac gacgctcctg
     7141 cgcgccctgg gtcggccggc caaaggcacg gacgcctacg tggaacagat cctgcacgag
     7201 aaacgtctgg aagaacgtca caccctgcaa tcgggcctgc cgatcgaatg gcctcaccgg
     7261 cccgacgccg ccgcggcctc cgccgaacgc gtggcggccc gttccatctc accgcagggc
     7321 caggcgggcg cccccgccat gacgctggaa gacctccggc aaaaagtgga gatcgtccgc
     7381 atcgacaaaa ccatggattg gaaaaaagcc aaattggaag tggacgcggt gcggcgcagc
     7441 cacgtcggcc gcgagttcct cgtcaactcc atcaaggcga atttcgtcgg ccgctggaac
     7501 atctcgctca tcctgcggga caaggagaac ggcgacatca tcggctacgt ggtgggcgtg
     7561 ccgcgcggca aacgcaagga acttcagacc ctttatcccg acatggacag ccggaccacc
     7621 ctgttcgtcg aagaagcggt ccacacccgc atgccgccgg agatccgcga actggcgccg
     7681 cagttgctgg acgaagagct ggccaaactc gcccagagcc tgggctacaa acagatcgtc
     7741 cgcatctcca aagaagccgc ggaatcgagc ccgctggtca ccagcgcgcc cgaacaggtc
     7801 tcaaccgccg tccagaccca ggcgctcatc gaggatttct gggcgacttt caaagaggtg
     7861 atgtccctgg ccgcggtcaa cgccatggtg cggcgcgagg cggaggtctc cctgcggcgc
     7921 atcggttacg gcaacctggc ggacctgctg agcgccggcg aaaaaacggt ggaagagctg
     7981 caaagcctcg tggacatctc ccaactttcc caggaggtgc tcttccgggt caaagaaatc
     8041 gccggtttca aacggatcct gcgggtcgaa gaagggtccg aagccatcga ggacaaactc
     8101 gtccgcgagc agatcaaccc ctatttctac gagaacggcc gttcctatct cagcgaaggc
     8161 aaaaatttcg acgccctgcg gtcgttcatg gcgaccctgg aactccggcc ggccgaaacg
     8221 actctctggc tggcgctggc cgacacgctc tacgccctgg gcctgaaacg gtccctgaag
     8281 acggcgtcga aactggcgtt cggcgaaaac ccgctcccgg ccacccccga agagctgcgc
     8341 gggattttcg acggccttct ggccgaactc ggggaaaaat atcccacgga accggcggtg
     8401 gacctgttgg gctacgccgc cgggcccgcc ttcgacgaac tgagccaacg ggaagacctg
     8461 accgcggaag aaacggcggc cctggtcgaa gtgacccaac tgcgtcaagg caagaaggac
     8521 atccagcaga tcatcagcta caacgaactc gtgaccatta aggaaatccc ggtggacgtg
     8581 gcgttgcggg tctattccta tatcaacgcg cggcgccgcg tcggcaaacc ggtgacctcc
     8641 ctcggcgccc tgatcgatct cgaaggcatg accgcgccgc gcctcctgaa actgatggaa
     8701 ctcttctacg tgcccggcgc cgagttggaa gacgcgatgt ggcgggtgcg ttacgaaaag
     8761 gaccaggcca aggcgaaata cgccgaggcc gagggggcgg aaaacctcca gcggagacag
     8821 gcgttcgagg cggccaaagg cgaactgaga cggctcctgg ccctcaacgg ggtcgtttcg
     8881 gccgtgcaga accgatccct ccgggaaaga cgcggcgcgc tgggacgcgc cttcgatcat
     8941 ctcttcggcg tcctcgtgaa agccgacccg gtggtgaaca cggtgaaagc ggcgctgggg
     9001 ctggcggcgg agaagaaaca gccgctcgtc ctcctgccgg aagaaaccgc cgaacgattc
     9061 gcggaactgc aggacgaact ggcgccgctg acggaccatt accggaccct gcgggaaacc
     9121 tacgttttga agaacaatta ccgtctctcc acggcggatc tccgcgccct gacggccgag
     9181 ttggagaaac cggtgcctct gacgctccgc gacggcacga ccgtcgagcg gtccctcatc
     9241 gatcggttgg aagacctggc caaggcgtat ccggaaatca gcctggaact catggagatg
     9301 ttggatgtgg tcctgttcta cgcctaccaa cagggcacga cgcccagccc ggatgccgtc
     9361 tccaaactga ccgaattcag ccgagccgtg atggactacc agaaaaaagc cgctccggcc
     9421 aaagccgctc cggagctccc cgaatttcac aaggagtcga aacccgccaa cacgagttat
     9481 tccgatgcgc tcatcgccga ccaggaagag gtcggcctcg tgaaactcag caaagcgatg
     9541 gaaaaagagt ggggacaggt caaactgctc atcatcgacc ggctcgccgg caaaaacgtt
     9601 cccaaagaca tcctggccat gctcaagacc gccaaattca aacgggaggc caaagagccg
     9661 aaaccctggt ccgcggtggt gggcggccgc atcgcctggg cgggcgcgta cgtcaccgaa
     9721 gaccgccggc tctacatccg cgggcacttc ctccaagccc tttccaagga agaactggcg
     9781 gacctcatcg ccaacacgtt gttggccagc atggaagaaa ccgccgcccc cgcccaacaa
     9841 cccgccgcga ccaa
//
LOCUS       DAOTFD010000072         9825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4879_length_9825_cov_4.106448, whole genome shotgun sequence.
ACCESSION   DAOTFD010000072 DAOTFD010000000
VERSION     DAOTFD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_9825_cov_4.106448"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..231)
                     /locus_tag="P5079_05840"
     tRNA            complement(159..231)
                     /locus_tag="P5079_05840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Thr,seq:ggt)
     gene            complement(300..725)
                     /locus_tag="P5079_05845"
     CDS             complement(300..725)
                     /locus_tag="P5079_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29546.1"
                     /translation="MRGAGPLLIKPKEQIGFILPAVVTVLLTVLVSYVIISVLRYAAV
                     STLQMNGASERVLERFIADTKIFVWIFWGLAGGLTLSALLWSWWFSFNLFGPLRRLEN
                     ELLSALKNEKKHVELRTRKSDALHPLGELLHQFLTRSKP"
     gene            complement(856..1611)
                     /locus_tag="P5079_05850"
     CDS             complement(856..1611)
                     /locus_tag="P5079_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRY29547.1"
                     /translation="MLRVIWERLKQGHRTIAYPKAPPALPERYRGCPVLSPEKCGADC
                     DRCVSVCPTQAIEKKGNSLRLDLGRCLFCGDCEEACSSGAIRFGQDHRLAVRNREDLM
                     MEGRALRLAESLDRKARRLFGRSLKLRQVSAGGCNACEADVNVLNTVVFDLGRFGIQF
                     VASPRHADGLLITGPVPANVRLALQKTYAAVPAPKIVIAVGACAVSGGPYYDHADVLS
                     GRSDLPPVDLFVPGCPPHPVTILDGLLRLLDRL"
     gene            complement(1619..3139)
                     /locus_tag="P5079_05855"
     CDS             complement(1619..3139)
                     /locus_tag="P5079_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRY29548.1"
                     /translation="MPAQTLLLRNGQAAARRAIPVLPFSAFRETLSSEVSAGGRLASL
                     FAWEEKPGRATLSAVVARGKEGVLSLFSTEVGDRYPSLTPECPQAHLFERELAEQWSV
                     VPDGHPWLKPVRFHSSYSSRPGTRDNAVVAEPLPGRTPFFRVEGDEVHEVAVGPVHAG
                     IIEPGHFRFQCHGEKVLHLEISLGYQHRGVERALLGGPTTRTISYMETMAGDTTVGHA
                     AAYCGVVEALAGVTAPPSAQMWRGVALELERLANHTGDLGALAGDVGYLPTAAYGGRL
                     RGDFLNMTAMLCGNRFSRGLLRPGGVAMRADGILTERLLEKLEAAFRDVRGAVSLLWD
                     TPSVMARFEETGVVSGEQAESLGLVGPPARAAGLVRDVRTEFPWGPYLAQPVPIAVQS
                     SGDVWARAAVRWTEIRRSVEFLRQVLSRPMTGSLREPMRPLAPDSVAVTLVEGWRGEI
                     CHTALTDPAGRFLRYKIVDPSFHNWMGLALALRGQEISDFPLCNKSFNLSYSGHDL"
     gene            complement(3143..4618)
                     /locus_tag="P5079_05860"
     CDS             complement(3143..4618)
                     /locus_tag="P5079_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY29549.1"
                     /translation="MIAALVLLPLLAGLAALLVRRDGPRRTVLLLTAFVHLGLSASAW
                     RRAPAPLWNGWLALDPAGRLFLGVTSLLFAVAAVYAVGFLAREEKGKQKDADEAGAFF
                     LNGPEAIFVGCLLFFFSAMTFVTVCQHFGLLWVAVEATTLASAPLIYFHRNHRSLEAV
                     WKYLVVCSVGIALALVGNLFLAVAAAPNGGPAVPLVLSEILNQAGHLNVPWLRAALVF
                     FFVGYGTKMGLAPMHTWLPDAHSESPSVVSALLSGALLNCAFLALWRAAQTGVAAGQA
                     AFVQDLFLILGLASLAVAAVFILGQTDFKRLLAYSSVEHMGVIAFGMGIGGSGVFAAL
                     LHSVNHSLVKGMLFLLAGNILAAYRTKAVAQVRGVSRLLPVTGPLWLAGFFAISGMPP
                     FGTFLSELLIVKAGLDSGRWGVTGVFLLLLTLVFIGMSRCFLNMAQGRPDGNGLVSPT
                     KEKRIAFLPSLFLGALSLWLGLTVPGFLQRLLQETAARWGG"
     gene            complement(4621..5259)
                     /locus_tag="P5079_05865"
     CDS             complement(4621..5259)
                     /locus_tag="P5079_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29550.1"
                     /translation="MSAWDQALWVAFILLNFLLLGTSRLSVCVRVLSAQGFLLAALPW
                     LSSSAITGHTVVLSAASALLKGLVFPWFFMRALDAAEVRREVEPYVGFGASLFVGLAA
                     LGFCVWLARALPHPSAVSNVALPASLFALMTGLFLIVSRKKAITQALAYLVMENGATA
                     LGMALLHWEPLVVELGILLDLFAAVFVMGIAVYHINREFDHIDTDRLNYLKD"
     gene            complement(5256..6173)
                     /locus_tag="P5079_05870"
     CDS             complement(5256..6173)
                     /locus_tag="P5079_05870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRY29551.1"
                     /translation="MADGSMTNVFLAAVLSPLLLGVINRTKAFFAGRKGVPLGQAYYD
                     AAKLLRKGMVLSRTTTPVFWLGPVAGLAAMTSALLFVPLGGLPAAVSFPGDWILLAGL
                     LALARFLTVLSALDTGSSFEGMGASREAFFASLTEPVFLSALAALSLKGGASSLNDLC
                     AFASTLFAENLPAAVLVAAALFIVYLTENARLPVDDPNTHLELTMIHEVMVLDHSGPL
                     FGYVVYGTAVKLWIFGSIVVSVCPLFRAASAPFGVLVALAGLLAVAVLTGVVESVMAR
                     LRLLRVPLLIAGAGVSAVVGAILLTGVGR"
     gene            complement(6178..8148)
                     /locus_tag="P5079_05875"
     CDS             complement(6178..8148)
                     /locus_tag="P5079_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY29552.1"
                     /translation="METLLAALLGILLSAVFGAISDRLPGRWRRLAPAGLVVSSLLGL
                     LPVLKTLLQGEISAFRLPWGVPGGSFSLLLDPLSAFFLLFVLGLSSLAATYAVEYLEA
                     GRTGFFWFFFGVLTVSMSMVTLARNAVLFLVAWEIMTLASFFLVTFEDEHLPVRRAGW
                     FYLAASHVGTAFLLLLFVLLGRGTPVLDFDLFSFPAAETLEAGVLFALALVGFGVKAG
                     FVPLHIWLPEAHPAAPSPVSALMSGVLIKMGIYGLVRVLGFWSVPSVAWGKILILVGL
                     SSGILGVLFALAQHDLKRLLAYHSVENIGIIALGLGLGVLGKSLNQPLLAVWGMGGGL
                     LHVANHAMFKSVLFLGAGAVARTTGTRDMDRLGGLLRKMPWTGGCFLVGAAAITGIPP
                     LNGFVSEFLIFCGAFHGLKAGGLPALFSYSVVMGLGLIGGLAAACFAKAFGAVFLGEP
                     RNGAEHAAREAGPLMRWPMGILAAVCVLVGLFPGVVVEALSPLLRQVSGLSPADFSPI
                     KETMTGTLEVVSRIAFVFLAGVLGLWGFAAWRARRGAVAKSVTWDCGFAAPSPRMQYS
                     ASSFAQPFTDFFRGVLRTRFPPTVLRDLFPTESALHTKTPDVFHEELYRPLFGRLKET
                     LLHGRRLQHGRLQLYVLYIVATLVALLVWSRG"
     gene            complement(8246..8740)
                     /locus_tag="P5079_05880"
     CDS             complement(8246..8740)
                     /locus_tag="P5079_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY29553.1"
                     /translation="MGNGTQEIGVASVDLKEAVSVENVLLGMGGGKKKDALEALFAFC
                     VRSGGLSPSQLKAALSGIFKREKVGTTGLGNGIAVPHVDVEGLKEPLVALGVSPKGIP
                     FESLDGEPAKLILLILSPAEDVSLRRKLVAQFCRLGRDPYFVGSILDAPSPKAVVDIL
                     RSEL"
     gene            complement(8805..9122)
                     /locus_tag="P5079_05885"
     CDS             complement(8805..9122)
                     /locus_tag="P5079_05885"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY29554.1"
                     /translation="MAFRIKADFLRALAHPTRLAVIEYLKKGDSSVGRMVGELGIEQS
                     ALSKHLGILRQAGILAARQEKANVYYSVKDRDIYEMLRSIAQILRKKLRESEKILSSL
                     GRI"
     gene            complement(9378..9451)
                     /locus_tag="P5079_05890"
     tRNA            complement(9378..9451)
                     /locus_tag="P5079_05890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9417..9419),aa:Gly,seq:tcc)
     gene            complement(9723..9796)
                     /locus_tag="P5079_05895"
     tRNA            complement(9723..9796)
                     /locus_tag="P5079_05895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9760..9762),aa:Arg,seq:acg)
BASE COUNT         2286 a         3320 c         2809 g         1410 t
ORIGIN      
        1 tcgtccctac gttcacgtgc ggcttcgtcc tctcaaattt cggtttcgac atgatcacaa
       61 ccctccttaa ggtttttcag acttcaaccg ccccgtcggc tgcggggacc acggccaaaa
      121 acgcggcgcc gttcgcttcg caaaaaacga caagagagag ctggggaccg ggattgaacc
      181 ggcgacctcc tccttaccaa ggaggtgctc taccaactga gctaccccag cgaaatcaaa
      241 cttcccgaag ggtgagaatt accacatttc gcaccggctg taaagcctca aaccgaacgc
      301 tacggcttgg accgggttaa aaactgatga agcagttccc ccaggggatg gagagcgtcc
      361 gacttgcgcg tacgcagttc cacgtgtttt ttttcgttct tcaaagcgga gagaagctcg
      421 ttctccaggc gccgcaacgg cccgaacagg ttaaaggaga accaccacga ccacaacagg
      481 gcggacagcg tcaaaccgcc ggccaggccc cagaatatcc agacgaaaat cttggtgtcg
      541 gcgatgaacc tctccaggac ccgctcgctc gcgccgttca tctgcagggt gctcaccgcc
      601 gcgtagcgga gcacggaaat gatgacgtag ctcacgagca ccgtcaacag gacggtcacc
      661 accgccggca ggatgaatcc gatctgttcc ttcggtttga tcaacaacgg ccccgcgccg
      721 cgcatacgcc gactcctgtg agtgggattt taccgtaacg ccgtcttccc gccggacgac
      781 aaatccgcct caactatagc acaaaattcg ggggataact accgcgcgcg gaagcgggaa
      841 aaccgtccgg tgggattaca acctgtcgag gaggcgaagc aacccgtcca ggatggtcac
      901 gggatggggc gggcaacccg gcacaaagag gtccaccggg gggaggtccg accggccgga
      961 gaggacgtcc gcatggtcgt aatacggccc tccggaaacg gcgcaggccc ccacggcgat
     1021 gacgatcttc ggcgcgggga ccgccgcata ggtcttttga agggcgagcc gcacgttcgc
     1081 ggggacaggg ccggtgatca aaaggccgtc ggcatggcgg ggagacgcca cgaattggat
     1141 cccgaaccgt cccaggtcga acaccacggt attgaggacg ttgacgtcgg cctcgcaggc
     1201 gttgcatcca cccgcgctca cctggcgcag tttgagggaa cgtccgaaaa gccgccgcgc
     1261 cttccgatcg agggattccg ccagccgcaa ggcccggccc tccatcatca ggtcttccct
     1321 gttcctcacg gccagccggt ggtcttgccc gaagcggatg gccccggacg aacacgcttc
     1381 ttcacagtcg ccgcagaaga ggcaccgtcc caggtccaaa cgcagggaat tccctttttt
     1441 ctcgatcgcc tgcgtcgggc agaccgagac gcaccggtcg cagtccgcgc cgcatttttc
     1501 cggcgaaagg accgggcaac cgcggtaccg ttccggcagg gccggaggcg ctttcggata
     1561 ggcgatcgtc cgatggccct gtttcagtcg ttcccagatc actcggagca tgacgtcctc
     1621 acaaatcgtg tccgctgtag gacaggttga aacttttgtt gcacaacggg aaatccgaga
     1681 tctcctgacc gcgaagcgcc agcgccaacc ccatccagtt atggaaggac gggtccacga
     1741 tcttatagcg caggaaccgg ccggcgggat cggtcaacgc cgtatggcaa atctctcccc
     1801 gccagccttc caccagagtc accgccaccg agtccggcgc caacggccgc atcggctccc
     1861 gcagcgatcc ggtcatcggc cgagaaagaa cctgccgcag gaattcgacc gaacgccgga
     1921 tctcggtcca ccgtacggcg gcccgcgccc aaacgtcacc ggacgattga acggcgatgg
     1981 gcaccggttg ggcgagataa gggccccaag ggaattccgt ccgcacgtcc cgcacgagcc
     2041 cggcggcccg ggcgggcggg cctaccagtc ccaacgactc ggcctgttcc ccggaaacca
     2101 cgccggtctc ctcgaaacgc gccatcaccg acggggtatc ccacagaagg gacaccgctc
     2161 cccggacatc ccgaaaggcg gcctccaatt tttccaacaa ccgttccgtc aaaattccat
     2221 cggccctcat cgccacgccg ccgggccgca gaagaccgcg ggaaaagcgg ttgccgcaca
     2281 acatcgccgt catgttcaaa aaatcgccgc gcaggcggcc gccataggcg gcggtgggca
     2341 gatatcccac gtccccggcc agggccccca gatcgcccgt gtggttggcc aggcgctcca
     2401 actccaaagc cactccccgc cacatctggg ccgacggcgg cgccgtcacc ccggccaacg
     2461 cctccacgac accgcaataa gcggcggcat ggcccacggt ggtgtcgccc gccatcgttt
     2521 ccatgtaaga gattgtccgg gtcgtcgggc cgccgaggag ggcccgttcg accccgcggt
     2581 gctgataccc caacgaaatc tccagatgca ggactttctc gccgtggcac tggaaccgga
     2641 aatgtcccgg ttcgataatc cccgcgtgca ccggccccac ggccacttca tggacctcgt
     2701 ctccctcgac gcgaaagaac ggggtgcggc ccggcaacgg ttccgcgacg accgcgttgt
     2761 ccctggttcc gggccgtgac gaataagacg aatggaaacg gacgggcttc agccaaggat
     2821 ggccgtcggg aacgaccgac cactgctcgg ccagttcccg ctcgaacaga tgcgcctggg
     2881 gacattcggg cgtgagcgag ggataacggt cccccacctc cgtcgaaaag agggacagga
     2941 ccccttcctt tccgcgggcc accacggccg acagggtcgc ccggcccggc ttttcctccc
     3001 aggcaaaaag ggaggccagc ctcccgccgg cggaaacctc cgacgaaagc gtttccctaa
     3061 aagcggagaa cggaaggacc gggatcgcgc gccgggcggc ggcctggccg ttacgaagaa
     3121 ggagcgtctg cgccggcatg gatcagcccc cccaccgagc cgccgtctcc tgcaagagac
     3181 gttggagaaa ccccgggacc gtcaaaccga gccaaagaga gagcgccccc aaaaacaggg
     3241 acggcaggaa agcgatcctt ttttccttcg tgggcgaaac aagtccgttc ccgtcgggcc
     3301 gcccctgcgc catattcaaa aaacaccggg acattccgat gaaaacaagg gtcaacaaaa
     3361 gaaggaacac ccccgtgacg ccccaccgac cgctgtcgag ccccgccttg acgatcaaga
     3421 gttcgctgag gaacgtcccg aaaggcggca tgccgctgat ggcaaaaaaa cccgccagcc
     3481 agaggggccc cgttacgggc aaaaggcgcg acaccccgcg cacctgcgcc accgccttgg
     3541 tgcgataggc cgccaggatg ttgccggcca gcaaaaagag catccctttc accagggaat
     3601 ggttcacgct gtgcaggagc gcggcgaaaa cgcccgaacc gccgatcccc atgccgaagg
     3661 cgatgacccc catatgttcc acgctggagt aggccagcaa ccgtttgaaa tccgtctgtc
     3721 ccaaaatgaa gacggccgcc accgcgaggg acgccagtcc caatatcagg aacaggtcct
     3781 gcacgaacgc cgcttggccg gcggccacgc ccgtctgcgc cgcccgccac aaagccagaa
     3841 aagcgcagtt cagcagcgcc cccgacaaaa gcgccgacac caccgacggc gattcgctgt
     3901 gggcgtcggg cagccaggtg tgcatgggag ccaggcccat cttggtgccg tatcccacga
     3961 aaaagaagac cagcgcggcc ctcagccagg gaacgttcag gtggccggct tggttcagga
     4021 tctccgacaa caccaacggc acggccggcc cgccgttcgg cgccgccgcg acggccagaa
     4081 acaggttccc caccagcgcc agcgcgatgc ccacggaaca caccaccaaa tatttccaca
     4141 ccgcctccaa cgaccggtgg ttccgatgga aatagatgag cggcgcgctc gccagcgtgg
     4201 tggcctccac cgccacccag aggagcccga aatgctggca gaccgttacg aaggtcatgg
     4261 ccgaaaaaaa gaagagcagg catcccacga agatcgcctc ggggccattc aggaagaaag
     4321 cccccgcctc gtcggcatct ttttgttttc ccttctcttc gcgggccaag aatcccacgg
     4381 cgtagacggc cgcgacggcg aagagaaggc tcgtcactcc gaggaaaagc cggccggcgg
     4441 ggtccaacgc cagccatccg ttccagagag gggccggggc ccgccgccac gccgacgccg
     4501 acaaccccag gtgaacaaag gccgtcagaa gcaacaccgt ccggcggggc ccgtcccgcc
     4561 ggacaagaag ggccgccagg cccgccaaca aaggaagaag aaccagcgcc gcgatcatcg
     4621 tcaatccttc aggtagttca accggtcggt atcgatatgg tcgaactccc ggttgatgtg
     4681 gtacaccgcg atccccatca cgaacacggc cgcaaacaag tcgagcaata ttcccagttc
     4741 caccaccagc ggttcccaat ggagcagggc catgcccagc gcggtggcgc cgttttccat
     4801 caccagatag gccaacgcct gcgtgatggc tttcttccgg ctgacgatca gaaacaatcc
     4861 cgtcatcagg gcgaaaaggg aggcgggcaa ggccacgttg gacaccgccg agggatgagg
     4921 caacgcccgc gccagccaga cgcaaaaccc caaagccgcc aggccgacga acagggaggc
     4981 tccgaaaccc acataaggtt ccacctcgcg ccgcacctcg gccgcgtcga gcgcacgcat
     5041 gaaaaaccag gggaacacga ggcccttcaa caacgcgctg gccgcggaaa gaacgacggt
     5101 atgtccggtg atcgccgacg acgaaagcca cggcagggcg gccaagagga acccctgggc
     5161 cgaaaggacg cgcacgcaaa cgctcaagcg gctcgtcccc aaaaggagga aattcaacag
     5221 aatgaacgcc acccacaagg cctgatccca ggcgctcatc gccccacccc cgtcagcaga
     5281 atcgcgccca ccaccgccga gaccccggcc ccggcgatca acaacggcac gcgcagcagc
     5341 cgcaaccgcg ccatgaccga ttccacaacg ccggtgagca ccgccaccgc cagcaatccc
     5401 gccagggcca ccagaacccc gaacggcgcc gaggcggcgc ggaacagcgg gcagaccgac
     5461 accacgatgg agccgaaaat ccataatttc accgccgtcc cgtacacgac gtatccgaac
     5521 agcggcccgc tgtggtcgag caccatcact tcgtggatca tcgtcagctc caaatgggtg
     5581 ttcggatcgt ccacgggcag acgggcgttc tccgtcagat agacgatgaa cagggcggcg
     5641 gccaccaaca ccgcggccgg aaggttctcc gcgaacagcg tcgaggcaaa ggcgcacaaa
     5701 tcgttcaaag aagacgcccc ccccttcaac gacagggccg ccagggcgga caggaacacc
     5761 ggttccgtca gggaggcgaa aaaagcctcc cggctggccc ccatcccctc gaagctggat
     5821 cccgtgtcca acgcggacaa cacggtcaag aaacgggcca aagccaggag gcccgccaac
     5881 aagatccagt cccccgggaa ggacaccgcc gccggcaatc cgccgagcgg gacgaacaga
     5941 agggccgagg tcatcgccgc cagtccggcc acgggtccca accaaaagac cggcgtggtg
     6001 gtccggctca acaccattcc tttccgcaac aacttcgccg catcgtaata agcctgcccc
     6061 aacggcacgc ctttcctccc cgcgaaaaaa gccttggtgc ggttgatcac tcccaacaaa
     6121 agcggggaga ggaccgccgc cagaaaaaca ttcgtcatcg aaccgtcggc catcccgtta
     6181 ccctctcgac cagaccagca acgccaccag agtggccacg atatagagga cgtagagttg
     6241 aagccggccg tgctgcagcc gacggccatg gaggagggtc tccttgagcc gcccgaagag
     6301 gggccggtaa agttcctcgt gaaagacatc gggggtcttg gtgtgcaggg cgctttccgt
     6361 cggaaacagg tcgcgaagaa cggttggagg gaaccgcgtc cgcagcacgc cgcggaaaaa
     6421 atccgtgaag ggctgggcaa aagaagaggc ggaatattgc atgcggggcg acggggcggc
     6481 gaaaccgcaa tcccacgtga ccgatttggc gacggccccg cgccgggccc gccacgcggc
     6541 aaacccccac aacccgagga cccccgccaa gaagacgaag gcgattcgag aaacgacctc
     6601 cagagtcccc gtcatcgttt cctttatcgg cgaaaaatcc gccggggaca gaccggacac
     6661 ctgccggaga aggggcgaga gcgcctccac cacgaccccg gggaacagtc ccaccagaac
     6721 acaaacagcc gccaaaatcc ccatgggcca ccgcatcagg ggtcccgcct cgcgcgccgc
     6781 atgttccgcc ccgttcctcg gttcacccag gaaaacggcg ccgaaggcct tggcgaaaca
     6841 ggcggcggcc aacccgccga tgagccccaa ccccatgacc acggaatacg agaacaacgc
     6901 cggaagcccc ccggctttca acccgtggaa agcgccgcaa aaaatcaaga actcgctcac
     6961 gaaaccgttc agcggcggga tgccggtgat ggccgccgcc ccgacgagga aacaaccgcc
     7021 cgtccacggc atcttcctca gaagtcctcc gagccggtcc atgtcgcggg tgccggtggt
     7081 gcgggccacc gcccccgctc ccaagaagag cacgcttttg aacatcgcgt gattggccac
     7141 gtgcagcagg ccgcctccca tcccccaaac ggccaagagg ggctgattca gactcttccc
     7201 caagacaccc aatcccaggc ccagggcgat gatgccgata ttctccacgc tgtggtaggc
     7261 caacaggcgt ttcaagtcgt gctgggccag ggcgaaaaga acgcccaaaa ttccggacga
     7321 gagccccacc aggatcaata tcttccccca cgccacggag ggcacgctcc aaaatcccag
     7381 cacgcgcacc agaccataga tgcccatctt gatgagcacg cccgacatca aggccgaaac
     7441 ggggctgggc gcggcggggt gggcctccgg caaccagata tgcagcggga cgaaccccgc
     7501 cttcacgccg aaaccgacga gcgccaacgc gaacagcacc ccagcctcca acgtctccgc
     7561 cgccgggaag gaaaaaagat cgaagtccaa gaccggcgtt ccgcgcccca acagcacaaa
     7621 gagcaggagg aggaaagcgg tgcccacgtg ggacgccgcc aggtagaacc atccggcgcg
     7681 ccgcacggga aggtgctcgt cctcgaacgt caccaagaaa aaggaagcca gtgtcatgat
     7741 ctcccacgcc accaggaaca acacggcgtt ccgcgccagc gtcaccatcg acatggaaac
     7801 ggtcaacacg ccgaagaaaa accaaaaaaa gccggtccgg ccggcttcca aatattccac
     7861 cgcgtaggtc gccgccaagg aggacagtcc caaaacgaac aggaggaaaa aagcggacaa
     7921 ggggtccagc agcagagaga aagaccctcc cggcacgccc cacgggagcc ggaacgcaga
     7981 aatttcaccc tgcaacagag ttttcagaac gggaaggagg cccaggaggg aagaaacaac
     8041 aaggccggcc ggggccagcc gacgccaccg gcccggcagg cgatccgata tcgcgccgaa
     8101 aacggcggag aggagtatcc ccaggagtgc cgcaagaaga gtttccactt attcagcccg
     8161 cctcaacgaa aggtctcgcc acggaggaaa acagaagctc ctctcgctca ttcgcccacg
     8221 ccgatttcaa atggaacaga gggagttaca gttcgcttcg aaggatatcg acaacggctt
     8281 tgggggacgg agcgtcgagg atgctgccga caaaataggg atcgcgaccc aggcggcaga
     8341 actgcgccac cagtttccgc cgcagggaca catcttcggc gggcgacaaa atgagcaata
     8401 tcagcttggc gggttccccg tcgagtgatt caaaggggat gcctttgggg gagaccccca
     8461 gggcgacgag gggctctttc agaccttcca cgtccacatg ggggaccgcg atgccgttcc
     8521 ccaaccccgt ggtgcccact ttttcccgct taaaaatacc ggacagcgcc gctttcagct
     8581 gggagggcga aaggccgccc gaccggacac aaaacgcgaa caacgcctcc agcgcgtcct
     8641 tctttttccc cccgcccatc ccgaggagga cgttctctac cgacacggct tctttcaagt
     8701 ccaccgacgc cacaccgatt tcctgcgttc catttcccac cggacgactc cctcccgcaa
     8761 tttttgcgat agatgtctaa cccgaccgaa aaccttggaa gttttcaaat ccttccaaga
     8821 ctgctcaaaa ttttttcact ctctctcaac ttcttgcgca atatctgcgc gatggaccgc
     8881 agcatctcgt aaatgtcccg gtctttcacc gaataataga cgttcgcctt ttcctggcgg
     8941 gcggccaaga tccccgcctg ccggaggatc cccagatgtt tcgaaagggc cgactgttcg
     9001 atgcccagct cgccgaccat ccggccgacg ctggaatcgc ccttcttcag atattcgatg
     9061 accgccaaac gggtgggatg ggccaaggcg cgcaaaaaat ccgccttgat tcggaatgcc
     9121 aaactgccgg caggaacttt ccccttaatg ctcatatgct cattatatca tattccaaac
     9181 acccgagaac cgtccccgcc caaaaagacc ccctcgttca agaggcggcc gggcggcccc
     9241 catcccatcc gacaaaccgg cgctatgcct ggcggcattg aagatcaagc tcgaaccgtc
     9301 aaattttggg caaaaaaatc cgcctttccg tcgagccaca ccttcaactt gtggccacag
     9361 aaaagcggac tggagattgg agcgggcgat gggaatcgaa cccacgtccg aagcttggaa
     9421 ggcttctatt ctaccattga actacgcccg ctaaaatgtt gtcactcgtc gaacatggtt
     9481 cgccgaaact atgttgaaac taggcaaccc cgcgcgattg tcatcgaatg ccgaaggcat
     9541 tcggaagggc gaaagcgggg cgaccgcaaa agctgaattt ttgttactgg cctctccgca
     9601 gagcgacagg ggccagaggc gccgccggac ggcgaccacc cctcagggct gtcccagcgt
     9661 tttgggtgag cgcttcgcaa cgaaccctgt tgggacgccc ggtttttccc ttttaaatta
     9721 aacgacccca gtaggatttg aacctacggc ctcttgcttc gtaggcaagc gctctatcca
     9781 actgagctat ggggtcaaaa atcgtcggat tcgagaattt atctg
//
LOCUS       DAOTFD010000073         9779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4906_length_9779_cov_4.305738, whole genome shotgun sequence.
ACCESSION   DAOTFD010000073 DAOTFD010000000
VERSION     DAOTFD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_9779_cov_4.305738"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1151
                     /locus_tag="P5079_05900"
     CDS             192..1151
                     /locus_tag="P5079_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRY29555.1"
                     /translation="MEPIDKNHPLARDLVLDELFDLTLYQRLQKITTGAPAKILAELI
                     PVETTHVNFWKSFFGLEINELNLLRKIKMALIVTACRLFGDTAIHMVLEGIEIYGVRK
                     YLAVWESYKDRPLGDAVRNVLNDEFGHEDEIVSQMTERKIDPEKIRTIFLGFNDGLVE
                     ILGAVSGFFAAFAETSLVLMAALSVAVAGSISMAAGVFVSSSSEYEVEKINEGKARFL
                     GQGTRAGDESGSPIRSAVIVGISYFIGAMIPVFPVFFGTQTVWYSLATAGVMIILVSL
                     VLSFLSGMRIRKRIFMNLVIISLAVGISYLIGMAVKNIWGISV"
     gene            complement(1170..2795)
                     /locus_tag="P5079_05905"
     CDS             complement(1170..2795)
                     /locus_tag="P5079_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29556.1"
                     /translation="MIHETSPKIRPELKGLFFPSGPVFDGAAVILALVSFGMSWVETL
                     VNQDSHHWGFMYSQALDLNRGLVPYKDVLIAYGVLTSRIQGFFLTVMGERLVSIGAAT
                     GLFYSTTLLLSYAVFRKFLTKPFALFSVLLVFLVHPYVIYPWSNYYAYPFELAALLVL
                     LGGFRSSVGGFFSGVLVGLAVLCRFSAAVSLVPPFVLFFAVDYAVRREQRNEILTTAL
                     FWSAGLFLPLAVFWGFLAHHRAVGEFLRQNSVMSREWREMGSFRLFLRFCWNVLSART
                     LPVRDVRSWLFYAVFIGNLVTVFHFLKKRGSVKDGSSGEGSLLLFSLAAVFGFMNSIH
                     IYEVFRLVNGASLGIGVLVWVVFSAYPGFGKRGKWLAWWSSIAVVCLLAGNLVFARNS
                     STFTPWTWEKMTGSGTARIQTGVFRGKRVSRAYREFYGDIATRLSAVDRSCPIVNVTA
                     DPLPVLLCDLPRLQRTPLYVHQMERPERLDEVRRLIGLRRVVLAGYFPLEVPGYRLLY
                     GAPWPEDVPWFDDEGLALFISVPEWALPPEPPS"
     gene            complement(2883..4784)
                     /gene="asnB"
                     /locus_tag="P5079_05910"
     CDS             complement(2883..4784)
                     /gene="asnB"
                     /locus_tag="P5079_05910"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY29557.1"
                     /translation="MCGIVGKIVWTPGGSVPKELLGKMAERLRHRGPDEGGIWAEGPV
                     GLAMRRLKVIDLASGQQPMSNEHCPHHGRTGTLRLIYNGEIYNFQSLRTELESRGHRF
                     RTASDTETILHAYEEFGEEAWDRLNGMFALALYAEREKTLTLVRDRMGVKPLYYRSTG
                     RSFSFASEIKALLEDPETSREWDLQALGEFFSLRYVPTPRSIFREIRKLEPGHVLKIR
                     DGRTEKRRYWTFSPPPAPRRPLSSYLEQLDSLLKESVRTHMVSDVPVGVFLSGGLDST
                     TVAAYVHETGRRLKSFTIYFPNESFSERREAALAAERYGLEHNEMEVHADLREAVDKL
                     SEVFDEPFADSSAIPVYYLSRFARSQVTVALSGDGGDELFAGYPTYIADRLSAYYRRF
                     PGMVHRLLLAMSRCLPVSYERISFDYRVKAFLHGARRAQPHAHFGWQEMLTPEEKSAM
                     VTPEVWREMGRHEADESFSAAYDEAGARNELEKMLYVDQRTHLLDEYLVKVDRVSMAH
                     SLEVRVPLLDRSLVEFAAGIPPAYKLSGFTTKYILRRMMRNRLPKEVVSMKKKGFSPP
                     LAHWLAGEWLPWAKDRLSPANLRKTGVLDPALPQKLLQEHADRKRDHYRRLWVLLCFM
                     SWFDKYGRK"
     gene            4881..5588
                     /locus_tag="P5079_05915"
     CDS             4881..5588
                     /locus_tag="P5079_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HRY29558.1"
                     /translation="MRPVEKHSWGLTTEKAEDLQRTLSARLTRQTPFKDAGDVRTVAA
                     VCVEQKKNDAPVSAAAAVFRLPDFQLLESSIVTEKPGKLFPYVPGLLSFRETPLFLKA
                     LKAVTDADVLLVPGHGLAHPRRFGIACHLGLLMDRPSIGCADEKIFGDFEEPPPGLEG
                     AYTLLRDGDEMIGAALRTRPNTAPLFVSPGHKMGVLMAVDIVMNCVRGNRAPEPLRLL
                     RQLLREQPPARGRKGKR"
     gene            5585..6430
                     /gene="amrB"
                     /locus_tag="P5079_05920"
     CDS             5585..6430
                     /gene="amrB"
                     /locus_tag="P5079_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY29559.1"
                     /translation="MKHLSDDRPARLAGTWYPADPQEIAGYLSDAAEEKDAVGALCPH
                     AGWTYSGKVAGAVYSRLPRADTFVLLGANHYGRGWPLSVYPSGAWQTPAGVLPVDADF
                     AAQLLEACPLLKADAAAHTLEHSLEVQTPFIAHRFPGTKIVPILLHDYSLSACRALAE
                     ALQKTVRNGKNRVILIASSDMSHVGEDYGQRPPKGMTAPAFALAQDEKALDRLRALDD
                     QGLLDAVTKNSISMCGAGPAAVMAAASRLLGAQKAETVMYRTSSDITKDTQSCVAYAG
                     VLATA"
     gene            6457..8178
                     /gene="polX"
                     /locus_tag="P5079_05925"
     CDS             6457..8178
                     /gene="polX"
                     /locus_tag="P5079_05925"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRY29560.1"
                     /translation="MLNKSFTDLLNEMAVLLELNQDNVFRIRAYQTAAQAVAALTSDI
                     ASLTREQLLEVSGIGKGIADKIEEFVKTGHVKEHDDLKEKFPAGLLDLLNVSGLGPKR
                     ARLLYDELKIDSFDKLKKAAAAGRLQKIPGFGEKIEQNILKGLHLAEQARSRMLLWEA
                     RRLVNDVLLQLRGVPGLLEIEPAGSLRRGKETVGDLDILCASRNPDALIERFTHLPSV
                     ERVLAAGATKSSVLLSGGVQCDLRVVAAGSFGAALQYFTGSKEHNVALRERALRMGYT
                     VNEYGLYRVSDKKQAKPVAGKTEQDIYKKLGLSFIPPELRENRGEIAAAEKNRLPELL
                     EESDVKGCFHNHTDLSDGTSSLEDMADAAEKKGWEWYAVGDHSQSLKVAGGMAPAAVR
                     KKIEKIRALNKKRRVCRLLSASEVDILADGRMDYADDLLAEIDVVTGSIHSGFHQSEE
                     QITGRLVKAMENPHVDCIAHLSGRLLGRREPYAFNTDKVLQVAERTGTALEINGQPDR
                     QDLTDINALAAKKRGIPMAVSTDAHSTEQLNYMSIAVTVARRAWLEKSDILNTKTYPE
                     LLEWLKD"
     gene            8190..8570
                     /locus_tag="P5079_05930"
     CDS             8190..8570
                     /locus_tag="P5079_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29561.1"
                     /translation="MRQTIFAVFLSGLLWPGLGQLYNRDFLKGFVLIGLTLLFGLSLF
                     LGAGLEIANRLPADLSTFDMTQARALSQELLKSNSGFFFTFNLLTMITWLYAVVDAYL
                     GAKERLSPPTPTDEPPPADDSDSE"
     gene            8641..9288
                     /locus_tag="P5079_05935"
     CDS             8641..9288
                     /locus_tag="P5079_05935"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRY29562.1"
                     /translation="MRGLDRRAAALGVPGAVLMENAGRAVADAVGTILNPPPASRIAV
                     FCGGGNNGGDGLVAARHLADAGFQPRVALALPPERFQGDALFHWKKLLDRKIPYAVFQ
                     SLQKLLEFLGDFSAAVDALLGTGFHGAPREPFRSLIVFLNGSSAPVTAVDVPSGLDAD
                     TGRVDDVAVRADLTVTLGLPKRGLVKKSARPWTGLLRVADIGFPPELTRDFLRRP"
     gene            9334..9567
                     /locus_tag="P5079_05940"
     CDS             9334..9567
                     /locus_tag="P5079_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRY29563.1"
                     /translation="MVTGLVLVRLNAGKEKQTLKAIKETKGVAHVSAVYGRWDLVVDL
                     EAEDMAHMTQIVVEKIRAIPGVNSTETLVTTAI"
BASE COUNT         2001 a         3193 c         2909 g         1676 t
ORIGIN      
        1 aaaggcgcat ccgcaaacgc ccgctgcgcg ggatatgcga ccactgcggc ggagagcttc
       61 aactgcgcat ccggggccgc gatacgcgga tcgtgtgcaa aaactgccac ctgccgccgg
      121 acaactgccg ctgccgcata aaaagatagt tctccccaac cgacgcccgc ctcttccgac
      181 aaggaggtcc catggaaccc atcgataaaa accatcccct cgcgcgcgac ctggtcctgg
      241 atgaactctt cgacctgacc ctgtatcagc ggctccaaaa gatcaccacc ggcgccccgg
      301 ccaagatcct ggcggaactc atcccggtcg aaaccacgca tgtcaatttc tggaaatcct
      361 tcttcggtct ggaaatcaac gaactgaacc tgctccgcaa gatcaaaatg gccctcatcg
      421 tgacggcctg ccgccttttc ggcgacaccg ccatccacat ggtgctggaa ggcatcgaga
      481 tctacggggt gcggaaatat ctcgccgtgt gggaaagtta caaggaccgg ccgctgggag
      541 acgccgtccg caacgtcctc aacgacgagt tcggccacga ggacgagatc gtctcgcaga
      601 tgacagaacg gaagatcgat ccggaaaaga tccggacgat cttcctggga ttcaacgacg
      661 gcctggtgga gattctgggc gcggtgagcg gcttcttcgc cgccttcgcc gagaccagcc
      721 tggtgctgat ggcggccctc agcgtggcgg tggccgggtc catctccatg gccgccgggg
      781 tcttcgtctc ttccagttcg gaatacgagg ttgaaaagat aaacgaggga aaagcccgct
      841 tcctggggca gggaacgcgc gcgggcgacg aaagcggctc gccgatccgc tccgccgtca
      901 tcgtggggat cagctatttc atcggggcga tgatcccggt gttccccgtc tttttcggga
      961 cgcagacggt gtggtattcg ctggcgacgg cgggcgtgat gatcatcctg gtttcgttgg
     1021 tgctgagttt tctttcgggg atgcggatcc gaaaacgcat attcatgaac ctggtcatca
     1081 tctcgctggc ggtgggtatt tcctatctca tcgggatggc ggtgaaaaac atctggggga
     1141 tttcggtcta aacagccgcc tcctcccaat caggacggcg gctccggcgg cagcgcccat
     1201 tccggaacgg aaatgaagag cgccagccct tcgtcgtcga accaggggac gtcctccggc
     1261 cagggcgcgc cataaagaag tctgtatccg ggaacctcca gcggaaaata ccccgccaaa
     1321 acgaccctcc tcaaaccgat cagtcgtcgg acctcgtcga gtctttccgg acgctccatc
     1381 tggtggacgt acaggggcgt ccgttggagc ctcggcaaat cgcaaagcag cacgggaagg
     1441 ggatcggccg tcacgttcac gatcgggcag gaacggtcga cggccgacaa cctcgttgcg
     1501 atgtccccgt aaaattcgcg atacgcccgg ctcacccgtt ttccccgaaa aacacctgtt
     1561 tggatccggg cggttcctga ccccgtcatt ttttcccagg tccacggggt gaacgtggag
     1621 ctgtttcgag cgaacaccaa attgcccgcc agaagacaga ccaccgcgat tgacgaccac
     1681 cacgccagcc atttccctct cttgccaaaa ccgggatagg ccgaaaaaac aacccacacg
     1741 aggaccccga tccccaacga agcgccgttc accaaacgga acacctcata gatatggatg
     1801 gaattcataa acccgaaaac ggccgcgagc gaaaacagca gcaggctccc ttcgccggag
     1861 gacccgtctt tgaccgaccc tctctttttc aaaaaatgaa acaccgtcac cagattcccg
     1921 atgaaaacgg catagaacaa ccacgaccgc acgtcccgga cggggagcgt ccgggccgag
     1981 agaacattcc aacaaaacct taaaaacagc cggaaagacc ccatctcccg ccattcgcgc
     2041 gacatcacgc tgttctgccg gagaaattct ccaaccgcgc gatggtgtgc cagaaagccc
     2101 caaaatacgg ccaagggcag aaacagcccc gccgaccaga acaacgccgt cgtcaggatt
     2161 tcgttccgct gttcccgacg gaccgcgtag tcgacggcga aaaacaggac aaacggcggc
     2221 acgagcgaca cggccgcgga gaaccggcaa aggaccgcca accccacaag gacgcccgaa
     2281 aaaaaaccgc ccaccgatga acggaaaccg cccagcaaca ccagaagagc cgccagctcg
     2341 aacggatagg cgtaatagtt cgaccaggga tagatgacgt aggggtggac cagaaaaacc
     2401 agaagaacgg agaaaagcgc gaagggtttc gtcaaaaact tcctgaaaac cgcgtaggag
     2461 agcaacagcg tcgtcgaata aaagaggccc gtcgccgcgc cgatggaaac cagacgctct
     2521 cccatcaccg tcaggaaaaa accctgaatg cggctcgtca gcaccccgta ggcgatcaaa
     2581 acgtccttgt aggggaccaa tcccctgttc agatccaggg cctgtgaata catgaaaccc
     2641 caatggtggc tgtcctgatt caccagggtc tcgacccagc tcatcccgaa actgacgagg
     2701 gcgaggatca ccgccgcccc atcgaagacg ggaccggaag ggaaaaagag ccccttgagt
     2761 tcggggcgga ttttcgggga cgtttcgtgg atcacggcgt tgtccatcgg gcgagcggat
     2821 tcgcctcctg tctcaaggcc tctcctcttt aagaagacga ccggcgggaa aacggggggt
     2881 aactattttc ttccgtattt atcgaaccag ctcataaagc acaagaggac ccacagccgg
     2941 cgataatggt cgcgcttccg gtcggcgtgt tcctgcagca gtttctgtgg aagcgccggg
     3001 tccaacacgc ctgtttttct caggttagcc ggcgacagcc ggtctttcgc ccacggcagc
     3061 cattcgccgg ccagccagtg ggccagcggg ggggaaaacc cttttttctt catggacacc
     3121 acttctttcg gcagccggtt ccgcatcatc cgccgcagga tgtatttggt ggtgaacccc
     3181 gagagcttgt aggccggcgg aatccccgcc gcgaattcca ccagggacct gtccagaagc
     3241 ggcacgcgca cttccagcga atgcgccatg ctgacgcggt ccactttcac caggtattcg
     3301 tccaacaggt gcgtccgctg gtccacgtag agcatttttt ccagctcgtt gcgggcgccg
     3361 gcctcgtcgt aggccgccga gaaactctcg tcggcttcgt gccggcccat ctcccgccaa
     3421 acctccgggg tgaccatcgc cgatttttcc tccggcgtca acatctcctg ccagccgaaa
     3481 tgcgcgtgcg gctgggcccg gcgcgcgccg tgcaggaagg ccttcacgcg gtagtcgaaa
     3541 ctgatccgct cgtaggatac cggcagacac cggctcatcg ccaggaggag gcggtgcacc
     3601 atgccaggga aacggcggta ataggcggac aggcggtcgg cgatgtaggt gggatacccg
     3661 gcgaaaagtt cgtcgccccc gtccccggag agcgccaccg tcacctggga ccgcgcgaac
     3721 cggctgagat agtagacggg gatggccgag gaatcggcga acggttcgtc gaacacctcc
     3781 gacagtttat ccaccgcctc gcgcaggtcg gcgtggactt ccatctcgtt gtgttcaagg
     3841 ccgtagcgtt ccgccgccag cgccgcctcg cggcgctcgg aaaaactctc gttcgggaaa
     3901 tagatggtga aacttttcag tcggcggccg gtctcgtgca cgtacgcggc cacggtggtg
     3961 gaatccaaac cgcccgacag gaaaacgccc accggcacgt cgctgaccat gtgcgtccgc
     4021 accgattctt tcagcaacga atccagctgc tccaagtagg aggaaagcgg ccggcgcggg
     4081 gcgggcggcg gagaaaacgt ccaatagcgg cgtttctccg tccgaccgtc gcggatcttc
     4141 aggacgtggc cgggttccag cttgcgtatc tcgcggaaga tggagcgtgg ggtggggacg
     4201 tagcggaggg agaaaaactc gcccagggcc tgcaggtccc actcgcgcga ggtttccggg
     4261 tcttccagca gcgccttgat ctccgaggca aaggaaaacg accggccggt ggaacggtaa
     4321 tacagcggct tgacgcccat cctgtcccgc acaagggtga gcgtcttctc ccgttcggcg
     4381 tagagcgcga gggcgaacat gccgttcagt cgatcccagg cctcctcgcc gaactcctcg
     4441 taggcgtgca ggatcgtctc cgtatcggaa gcggtgcgaa aacggtgtcc gcgggactcc
     4501 agttccgtcc gcagactttg gaaattgtag atctcgccgt tgtagatgag ccgcagcgtc
     4561 ccggtcctgc cgtgatgggg acagtgttcg ttggacatgg gctgctggcc ggacgccagg
     4621 tcgatgacct tgagccggcg catggccagc cccaccggtc cctcggccca gatcccgccc
     4681 tcgtccgggc cccggtgacg caggcgttcc gccattttcc caagcagctc tttgggaacg
     4741 gacccgcccg gcgtccagac tatttttccg acaatgccgc acatgggcaa ggttttatca
     4801 tttttctccc gggccaacaa cggcacgcct cgacggcacg gcgacaccgg aaacaaactt
     4861 ttatattgta aaatcacgcc atgcggcccg tggaaaaaca ctcctgggga ctgacgacgg
     4921 aaaaggcgga agaccttcaa cggacgcttt ccgcccggct cacccggcaa acgcctttca
     4981 aggacgccgg agacgtccgc acggtggcgg cggtgtgcgt cgaacagaaa aagaacgacg
     5041 cccccgtctc cgcggccgcc gcggtgttcc gcctgccgga tttccagctc ctcgaatctt
     5101 ccatcgtcac cgagaagccg gggaaacttt tcccgtatgt ccccggcctg ttgtcgttcc
     5161 gtgaaacacc gctgttcctg aaagccctca aagccgtcac ggacgcggac gtcctgctgg
     5221 tgccgggaca cggactggcg catccgcgcc ggttcggcat cgcctgccac ctcgggctgt
     5281 tgatggaccg cccgtccatc ggctgcgccg acgaaaaaat tttcggcgat ttcgaggagc
     5341 cgccgcccgg gctggaaggc gcttataccc tcctgcggga cggcgacgag atgatcggcg
     5401 ccgcgttgcg cacccggccg aacacggccc cgttgttcgt ctcccccggc cacaaaatgg
     5461 gcgtgctgat ggccgtggac atcgtgatga actgcgtgcg ggggaaccgt gcgccggaac
     5521 cgctgcgctt gctgcggcaa ctcctccgtg aacaacctcc ggcccgagga cgaaagggga
     5581 aaagatgaaa cacctttccg acgaccgccc cgcccgactg gccgggacat ggtatcccgc
     5641 cgaccctcaa gagatcgccg gctatctctc cgacgccgcc gaagagaagg acgcggtcgg
     5701 cgcgctctgc cctcacgcgg gctggaccta ttccggcaaa gtggcgggcg cggtctattc
     5761 ccgtctgccc cgggccgaca ccttcgtcct cctgggcgcg aaccactacg ggcgcggctg
     5821 gcccctgagc gtttatccgt cgggcgcctg gcaaacgccg gccggcgtcc tccccgtgga
     5881 cgcggatttc gccgcccagc tcctggaggc ctgtcccctt ctgaaagccg acgccgccgc
     5941 ccacacgctg gaacattccc tcgaagttca gacgccgttc atcgcccaca ggttccccgg
     6001 aaccaagatc gtccccatcc tcctgcatga ttactccctc tccgcgtgcc gcgccttggc
     6061 ggaggccctg caaaaaaccg tgcgcaacgg aaaaaaccgg gtcatcctca tcgcctcgtc
     6121 ggacatgtct cacgtggggg aggattacgg ccagcggccg cccaaaggga tgaccgctcc
     6181 ggcgttcgcc ctggcacagg atgaaaaagc cctggaccgt ctccgggctc tggacgacca
     6241 ggggctgctc gacgcggtga cgaagaactc catctccatg tgcggcgccg ggccggcggc
     6301 ggtgatggcc gccgcctccc gcctcctggg cgcccaaaaa gccgaaaccg tgatgtaccg
     6361 cacctccagc gacatcacca aggacacgca gagctgcgtc gcctacgccg gcgttctggc
     6421 gacggcttag ccttcccgaa tttctataat ggaatcatgc tgaacaagag tttcaccgac
     6481 ctgctcaacg agatggcggt cctgctggaa ctgaaccagg acaacgtctt ccgcatccgc
     6541 gcctaccaaa cagccgccca ggcggtggcg gcgttgacgt cggacatcgc ctccctgacg
     6601 cgagaacagc tcttggaggt gagcggcatc ggcaaaggca tcgccgacaa gatcgaggaa
     6661 ttcgtcaaga ccgggcacgt caaggagcac gacgacctga aggaaaaatt cccggccggc
     6721 ctgctggact tgctgaacgt ctccggtctc ggccccaaac gcgcccgact gctttacgac
     6781 gaactgaaaa tcgattcgtt cgacaagctc aaaaaagccg ccgccgcggg ccggcttcaa
     6841 aagatccccg gtttcggcga aaagatcgaa cagaacatcc tcaaggggct ccacctcgcc
     6901 gaacaggcca ggagccggat gctcctgtgg gaggcgcggc ggctggtcaa cgacgttttg
     6961 ctccaactcc ggggcgtccc cggccttctg gagatcgagc cggccgggtc cctgcgccgg
     7021 gggaaagaaa cggtgggaga cctcgacatc ctctgcgcct cccgcaaccc ggacgccctc
     7081 atcgaacgtt tcacccattt gccgtcggtg gaacgggtct tggcggcggg ggcgaccaag
     7141 tccagcgtgt tgctctccgg cggagtgcag tgcgacctgc gcgtggtggc ggcggggtct
     7201 ttcggcgccg cgcttcaata tttcaccggt tccaaggaac acaacgtcgc cctgcgcgaa
     7261 cgcgccctgc ggatgggcta cactgtcaac gaatacgggt tgtaccgcgt ttccgacaaa
     7321 aaacaggcga aacccgtggc ggggaaaaca gaacaggata tctacaagaa actcggcctg
     7381 tctttcatcc cgccggaact gcgcgaaaac cgcggggaga tcgcggccgc cgaaaaaaac
     7441 cgtctgcccg aactactgga ggaatccgat gtgaaaggct gtttccacaa ccacaccgac
     7501 ctttcggacg gcacctcctc cctggaagac atggccgacg ccgccgagaa aaaaggctgg
     7561 gaatggtacg ccgtgggcga ccattcccaa tccctgaagg tggcgggcgg catggcgccg
     7621 gccgcggtgc ggaaaaaaat cgagaaaata cgcgccttga acaagaaacg ccgcgtctgc
     7681 cgcctgctca gcgcctccga ggtggacatc ctcgccgacg gccgcatgga ttacgccgac
     7741 gacctcctgg cggaaatcga cgtggtgacg ggttccatcc acagcgggtt tcaccaaagc
     7801 gaggaacaga tcaccggccg cctcgtgaaa gccatggaga acccgcacgt ggactgcatc
     7861 gcccacctca gcggccgcct gctgggacgg cgggaaccct acgcgttcaa cacggacaaa
     7921 gtcctgcagg tcgccgaacg caccgggacc gcgctggaga tcaacggcca gccggaccgg
     7981 caggatctca ccgacatcaa cgccttggcg gcgaaaaaaa gagggatccc gatggccgtc
     8041 agcaccgacg cccactccac cgaacagttg aactacatgt ccatcgccgt caccgtggcg
     8101 aggcgggcat ggctcgaaaa atcggatata cttaacacca aaacctatcc ggaactcctg
     8161 gaatggttga aggactgagg ggggaatcca tgagacagac catcttcgcc gtttttcttt
     8221 cggggctgct gtggccggga ctcggccagc tctacaaccg ggacttcctg aaaggattcg
     8281 tcctcatcgg gctcaccctg ctcttcggcc tctctctctt cctcggcgcg gggctggaaa
     8341 tcgccaaccg cctgcccgcc gacctttcca cttttgacat gacgcaggcc cgcgccctgt
     8401 cgcaggaact gctgaaatcc aactcggggt tcttcttcac cttcaacctg ttaaccatga
     8461 tcacctggct ctacgccgtg gtggacgcct acctgggagc caaagaacgc ctgtcgcccc
     8521 cgacaccgac ggacgagccc ccgccggcgg acgattccga ttccgaataa acgacccgcc
     8581 cgcgcctccc ggtcgcgcct ccggccgccg acggcctatc gttgcatcgg cgtggagccg
     8641 atgcggggat tggaccggcg cgccgccgcg ctcggcgtgc ccggcgcggt gctcatggaa
     8701 aacgccggcc gcgccgtggc cgatgccgtc ggcacgatcc tgaacccgcc gccggcctcc
     8761 cgcatcgccg tgttctgcgg cggcgggaac aacggcggcg acgggctggt ggcggcgcgc
     8821 catctggcgg acgccggctt tcagccccgc gtcgccctcg ctcttccgcc ggaacgtttt
     8881 cagggggacg ccctcttcca ctggaagaaa ctgctcgacc gaaaaatccc ctatgccgtt
     8941 tttcaatccc tccaaaagtt gttagaattt ctcggggatt tttcggcggc ggtggatgcc
     9001 ctgctgggca cgggtttcca cggcgcgccg cgcgaaccct tccgttccct gatcgtcttc
     9061 ctgaacggct cgtccgcgcc ggtgacggcg gtggatgtgc cgtccggcct cgacgcggac
     9121 acgggccgcg tcgacgacgt ggcggtgagg gccgacctga cggtgacgct gggcctgccc
     9181 aagcgggggc tggtcaagaa aagcgcgcgg ccgtggaccg gtctcctgcg ggtggccgac
     9241 atcgggttcc ctccggaatt gacgcgggat ttcctccgcc ggccgtgacg ggcggacgtc
     9301 tggttttgaa actttcgtag gaggtagaaa aacatggtga caggtctggt gttggtacgg
     9361 ctcaacgcgg gaaaagagaa acaaacgctc aaagccatca aggagacgaa gggggtggcc
     9421 cacgtctcgg cggtctacgg tcgatgggac ctggtggtcg atctggaagc cgaagacatg
     9481 gcccacatga cgcagatcgt ggtggaaaag atccgcgcca tcccgggtgt caattccacg
     9541 gaaactcttg tcacgaccgc catttagaat cgtccaccga aatccgttcg ccggccggcg
     9601 ccccgcggcg cccgcgccgt tcttttccaa aattcgcgtc gcgttcttct ggggcctcct
     9661 tatgaccttg gccatcccgc ttccctctca tgccgtcgcg gtaaaacgct tgccgaacgg
     9721 ccttcgctac gtcgtcctcg aagaccacgc cgccccggtg gtgtccctgc agatctacg
//
LOCUS       DAOTFD010000074         9733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_4937_length_9733_cov_4.460116, whole genome shotgun sequence.
ACCESSION   DAOTFD010000074 DAOTFD010000000
VERSION     DAOTFD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_9733_cov_4.460116"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1142
                     /gene="mdh"
                     /locus_tag="P5079_05945"
     CDS             195..1142
                     /gene="mdh"
                     /locus_tag="P5079_05945"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRY29564.1"
                     /translation="MRRKITVVGAGNVGATLAQRLAEKELADVVLLDIPQTEGMPAGK
                     ALDILQAGSVYGYDTKLTGATDYAAAEGSDIVVITAGVPRKPGMSRDDLLSTNAKIVK
                     AVSEQVARSSPNAVVIVVSNPLDAMAYVALRTTRFPEERVLGMAGVLDSARMAVFVAQ
                     ALDVSVEDVRPMVLGGHGDTMVPLPRYTTVAGVPVTELLSKEKIDAIVQRTRDGGAEI
                     VKLLKTGSAYYAPSASVVAMVESILKDKKRILPCAAYLNGEYGVRGVFVGVPVKLGAR
                     GREEVLQLNLTVEERVALQKSVDAVRELVEAMQRLGVAG"
     gene            1277..1507
                     /locus_tag="P5079_05950"
     CDS             1277..1507
                     /locus_tag="P5079_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29565.1"
                     /translation="MTTETIQCGKRVYMFESRITKNGHKYVMITEKSSGRLSKVMVFH
                     DHFDIFCKAIQRAMQAEPTPVVPAETEKAFSH"
     gene            1734..2420
                     /locus_tag="P5079_05955"
     CDS             1734..2420
                     /locus_tag="P5079_05955"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRY29566.1"
                     /translation="MATVRTATSDEYGTKILDFLHYASIESSQIGGVALASVVPPLDG
                     VFLDAVKKYFKTTPLVVGPGVKTGMNILYDNAAEVGADRIVNALAAFDRLGGPCIVVD
                     FGTATTFDCVNRKGDYLGGAIAPGPLLAAASLWEKTAKLPLLGAFRKPPSALGKNTHD
                     SMSSGLFYGYIGLTEELLRQLKKEVGGEPKIMATGGLAGLLAPYIKAIKKVDPDLTLE
                     GLRLVWERNL"
     gene            2570..2950
                     /locus_tag="P5079_05960"
     CDS             2570..2950
                     /locus_tag="P5079_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29567.1"
                     /translation="MQAKNILVVDDDVSNTDIVKLILESHGHRVSVSYSGKDAMQKVV
                     QVRPDLAVLDVKMPDVDGYAVCKFIKGHPALSPVKVILLTSQDLEKNVRNAEEAGADA
                     FLEKPCSVEKLIETVNRLLALRPA"
     gene            complement(2919..3476)
                     /gene="def"
                     /locus_tag="P5079_05965"
     CDS             complement(2919..3476)
                     /gene="def"
                     /locus_tag="P5079_05965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY29568.1"
                     /translation="MAAHRIILFPDPLLKQPSRPVDRADAAAAQLLTDLTDTLHRFPG
                     VGLAAPQIGVLQRAVVVDITRSTRREKKPPRHHGLIALLNPRLLRREGELVFREGCLS
                     VPDFLGNVRRAAWIEVEGMNARGETVKLDTDGFEAVALQHEMDHLDGVLFLDRVENVK
                     TDLFRRKNYAPRPPASTPAATPEAG"
     gene            complement(3495..4073)
                     /locus_tag="P5079_05970"
     CDS             complement(3495..4073)
                     /locus_tag="P5079_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRY29569.1"
                     /translation="MPRRLCYNQNMPYYLLTAAGPDKAGIVAAAAEILYELGCNLEDS
                     AMTRLRGEFAILLIFTAPAKTSLTELRRRLQRLEKSHGLTVQLKPMKPRETLEPPRPQ
                     RLCLVTVYGADKPGIVYRVTRLLSKNGVNITDVTTHRTAAGTAKGAAGYILYLEGELA
                     GADTDRLQGLLQDLGRELGVTVALKPVDAEPL"
     gene            4113..6158
                     /locus_tag="P5079_05975"
     CDS             4113..6158
                     /locus_tag="P5079_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY29570.1"
                     /translation="MDKSFVDRLRRAVGRGNVLTDPASLLVYSYDAATDKALPGAVVK
                     PHDTNEVAAVVRLAAEAGVPFVARGAGTNLCGGSIPADGGLVIHLSGLNRILRIDPIG
                     RRALVEAGVVNLHLQNALAPHGLFYAPDPASQKACTLGGNAATNAGGPHCLKYGVTSH
                     HVLGLELVLPDGRVVNTSVADGGYDLTGLFIGSEGTLGIITRMEVRLLPLPEAVQTLL
                     ASFPSLEDAVRAVTAIVSAGIVPSTLEVMDGVTLRAVEAFVHAGYPVEAGAVLLVELD
                     GPAARIAEDSARVREFVRRNRGGDVREARDEGERQKLWEGRRGSYPAMARLAPNVLVE
                     DGVVPRTRLPEAVRRIRAVADRENLRMGLIAHAGDGNLHPNIVFDERDRAQTERVRAA
                     GHEMMRVCVELGGSISGEHGIGLGKREAMRRLFTPETLEVFRALKDAFDPRRLCNPDK
                     IVPPPAAAGEEKSEAPSPVDLPDNGVLSAESVPRVRSLLASACAAGRKVVLCGSGRSA
                     GPTDGDVLALRLDGLTGVVEHDRDNFTLTVRAGMPLKDVRAYLTPFGQYLHLSGEGTL
                     GGVLSSCPAGKESVRDQIIGLKAVLADGRLVSFGGKVIKNVAGYDAAKLFIGAWGTLG
                     AVVEVTLRLFPSPALEKIPSAPAEDFSWMASPLHRRVKQAFDPAGVLLPPPGRGATK"
     gene            6155..7480
                     /locus_tag="P5079_05980"
     CDS             6155..7480
                     /locus_tag="P5079_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRY29571.1"
                     /translation="MKDADKPVLEPRWAELSSKKDEAGYEAALHCNRCGFCTSFCPTY
                     LATGDEGRSPRGRNQTFRALLEGRLKDPVQGGDLFDTCLLCGICTSVCFAEVPTAKLM
                     SAAREKVMQRAGEPWLMRGLLRSLLPRPRLFEFFLQVLFWGKRLGVSRALNRLGVLGL
                     LDRRLAAAEELTDSVPRRFLRDLLPNAPPDVDVVQFVACGPSYAMPAVGEATHRLVRA
                     AGKSVGRVDNVCCGLPALSLGDSAAARVLAEKNIAELEKYPRAVVLADDSSCAATLKE
                     YPSLFEEGEWRRRAEAVSRRTKDLSEWLAENRPTGTPLPPTVVTYHDPCKARYGQKIV
                     DAPRALLAATPGVDYRELPEADQCCGGGGTYSFLQPEISRQVLSRKIRNIESTGAAVV
                     LTSAVSCLLQIRAGLKWTGSRVRAFHLSEFLSPPPASDETGFFGPKTGL"
     gene            7678..>9733
                     /locus_tag="P5079_05985"
     CDS             7678..>9733
                     /locus_tag="P5079_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29572.1"
                     /translation="MTFDWAYRSKSIARKSVALFIVTAFVFTTVSEPWAQSSFWEQQR
                     SSRQNLYKQTDPQTAEAKALLSSVSWPKEAAHPLFGSFKLSSQLGSILETHLAASSEL
                     EPEKIRPLTLYHIQDAHGVYGAQYNASQIIDGLAAAFKEQDQPKAPLLVCQEGGLKSV
                     NTDWLSSFPFPEIKREVAHAHLRMGNLTGEEYLALVRLPGFVKLRGVENKDLYFDNLD
                     SRRNTIEARLQASKYIASLQARMNVVKENIYPSSLLDLDRKFRAYSDKKITFVQYLQE
                     LARRSSSPISRGEFPNVHATLLLSDMESVLDLDALREERTTLVDVMAEHLSLDSLKEL
                     VAKASEMRLGHVNPRVFYQGLVSIAGLLRQKGVVVPTDNLRQYVAYLELMERVNHEQL
                     LLETDQLKKRLLEHYAYNDQIWRLVNLDQRLQQEADLWGLAMGPDQFAGYRKEGPQDF
                     IGVEKYLLAQERSLGLSPVPVSEPVNLQPWLEKRPLIHRYYEFALERNLVLVQNALNA
                     MKETGASRAVLVAGGFHTQGMTDLLRQMGVNYVVIQPRFEAYQPVAEDQLLQISYSYD
                     FSESVGSVREQNRLDITGPLAQDLSKQIALQESIDGVVSMVLSRFGNERAANAEALEK
                     WWAEARGIIPIDVRRAIELEKGKSRQVVLFGRAAGEPVALVYDFRNKIMVLIGENSDL
                     SDA"
BASE COUNT         1762 a         2973 c         3178 g         1820 t
ORIGIN      
        1 cccctatgcc gaactctacg aggcccggcc gacgccgccc cctcctcgac ggtaaaatcg
       61 aatccgtgac cggtttgacg agtttgccga aacgtgttca aaatcaccga taaaatgcgg
      121 tgaaatattg tatactgcct ccgtttgggg cagatactta tagtcaacgg tttttgaggg
      181 gtcggggagg atttatgcgt cggaagatca cggtggtggg ggccggcaac gtcggggcga
      241 ctttggccca gcggttggcg gaaaaagagc tggcggatgt ggttcttctg gatatccccc
      301 agaccgaggg gatgccggca gggaaagctc tggatatcct tcaggcgggg tccgtgtacg
      361 ggtacgacac gaagctcaca ggggccaccg actacgccgc cgccgagggg tccgatatcg
      421 tcgtcatcac cgccggggtc ccgcgcaagc cggggatgag ccgggacgac ctcctctcca
      481 ccaacgccaa gatcgtcaaa gccgtgtcgg aacaggtggc ccgctcctcc cccaacgcgg
      541 tggtgatcgt ggtgtcgaac ccgctcgacg ccatggccta cgtggccctc cgaaccacgc
      601 gttttccgga agagcgggtg ttgggaatgg cgggggtgct cgactccgcg cgcatggccg
      661 ttttcgtggc gcaggcgctg gatgtttccg tcgaggatgt gcggcccatg gtgctggggg
      721 gccacgggga caccatggtg cctctgccgc gctacaccac ggtggccggg gtgccggtca
      781 cggaactttt gtccaaggaa aagatagacg ccatcgtgca gcgcacgcgc gacggcggcg
      841 ccgagatcgt caaactgttg aagaccggct ccgcctatta cgcgccctcg gccagcgtgg
      901 tggccatggt ggaatccatt ttgaaggaca agaaacgaat tcttccctgc gccgcttacc
      961 tcaacggcga atacggcgtg cggggggtgt ttgtcggcgt cccggtgaaa ctgggtgccc
     1021 gcggccgcga ggaggtcctt cagctcaatt tgaccgtcga ggaacgcgtg gccttgcaga
     1081 agtcggtgga cgcggtgcgg gaattggtgg aggccatgca acgtctgggg gtggccggct
     1141 gacggaggga ctgccgggat ttttgagggg gcggttaacc gcgggggaac agggcaggaa
     1201 tcgacagggt ggccggttgg cgataggagg gagttggaag gcgggtcaga gttcatccgg
     1261 cgggagatga ggaacaatga ccactgaaac catacagtgc gggaaacggg tgtatatgtt
     1321 cgaatcgcgc atcaccaaga acgggcacaa atacgtcatg atcacggaaa aaagctcggg
     1381 ccgattgtcg aaagtgatgg tgttccacga ccatttcgac attttctgta aggcgatcca
     1441 gcgcgccatg caggccgaac cgacgccggt ggtccccgcc gagacggaaa aagcgttcag
     1501 ccactaacgg ggcgggcgcg gccggacgcc gggccgcgcg gtctgcgggg attgatgtcg
     1561 gcgtctaaaa gtgaaatcat atcgcacggg ccggaacatc catgccggcc cctgtcctat
     1621 cggaatccat ttcgcatgct gctgacaata gatatcggca acacccagat cacgctcggt
     1681 gtttttcgca ccgaccaggg ccggcccctc aagggggtcg tctcttctgg cgcatggcca
     1741 cggtccgcac cgccacctcc gacgaatacg gaaccaagat cctcgatttc ctgcattacg
     1801 cctccatcga atcgtcccaa atcggcggcg tggccctggc cagcgtggtg ccgccgctgg
     1861 acggcgtttt cctggacgcc gtgaagaaat atttcaaaac caccccgctc gtggttggcc
     1921 ccggtgtgaa aaccgggatg aacatcctct acgacaacgc cgccgaggtg ggggccgacc
     1981 gcatcgtcaa cgcgctggcg gccttcgacc gtttgggcgg gccgtgcatc gtggtggatt
     2041 tcggcaccgc caccactttc gattgcgtca atcggaaagg ggattatctg ggcggcgcca
     2101 tcgcccccgg ccccctgctg gccgccgctt ccctgtggga aaaaaccgcc aagctgcccc
     2161 tcctcggcgc cttccggaaa ccgccttccg ccctgggcaa aaacacccat gacagcatgt
     2221 cctccggcct tttctacggc tacatcggcc tgaccgagga gcttctccgc cagctcaaaa
     2281 aggaggtggg gggggaaccg aaaattatgg ccaccggggg gttggccggt ctcctggccc
     2341 cttacataaa agccattaaa aaggtggatc cggacctgac tttggagggg ctccgactcg
     2401 tttgggagcg caacttatga cgaataacgt ggttttttgt ggataaactt gtgagtaaag
     2461 gccataaaaa cgaaaaataa tcggtttaaa gacaaaaagg cctttatttt gattttttct
     2521 cttttaggga tccgagtcga ggtccgcccg tcggcggagg gatgatttga tgcaggcgaa
     2581 aaacatcctc gtggtggacg acgatgtgtc caacacggac atcgtcaagc tcattttgga
     2641 gagccacggg caccgtgtca gcgtttccta cagcggcaag gacgcgatgc aaaaagtcgt
     2701 tcaggtgcgg ccggacctgg cggtgttgga cgtgaaaatg ccggacgtgg acggctacgc
     2761 ggtgtgcaag ttcatcaagg ggcatccggc gttgagcccg gtcaaggtga tccttttgac
     2821 gtcgcaggat ttggagaaga acgttcggaa cgccgaggag gccggggcgg atgctttttt
     2881 ggagaagccc tgcagcgtgg agaagctgat cgaaacggtc aaccggcttc tggcgttgcg
     2941 gccggcgtag aggccggcgg ccgaggggcg tagttcttcc gtcgaaaaag atccgtcttg
     3001 acgttctcca cgcggtccag gaacaaaacc ccgtccaggt gatccatctc gtgttgcagc
     3061 gccaccgctt caaacccgtc cgtgtccagt ttcaccgttt ctccccgggc gttcatccct
     3121 tccacctcga tccaggccgc ccgccgcacg ttccctaaaa aatccggaac agacagacat
     3181 ccctcgcgga aaaccagttc tccttcccgc cggaggaggc gggggttgag cagggcgatg
     3241 agcccgtgat gacggggagg ctttttctcc cggcgggtcg aacgggtgat gtccaccacc
     3301 accgcccgtt gcaacacccc gatctggggc gccgccagcc ccacgccggg gaaccggtga
     3361 agggtgtcgg tgaggtcggt gagcagttgg gccgccgccg catcggcgcg gtccacgggg
     3421 cggctgggct gtttgaggag agggtcggga aagaggatga ttcggtgtgc ggccacgggc
     3481 acccccgggg gctcttaaag gggctcggcg tccaccggtt tgagcgccac cgtcacgccc
     3541 agttcgcgcc ccaggtcctg cagaagaccc tggagccgat ccgtgtccgc cccggcgagt
     3601 tccccttcca gatagaggat gtatcctgcc gcgcctttcg ccgtcccggc ggcggtgcgg
     3661 tgggtggtga cgtcggtgat gttgaccccg ttcttcgaca gaagccgggt gacccgatag
     3721 acgatgcccg gcttgtccgc cccgtagacc gtcacaagac agagacgttg aggccgcggc
     3781 ggctccagcg tctcccgggg tttcatgggt ttgagttgca cggtcaaccc gtgcgatttt
     3841 tccagccgct ggagccggcg gcgaagttcg gtgagggacg ttttggccgg cgccgtgaag
     3901 atcagcagga tggcgaactc gccgcgcagg cgcgtcatgg cggagtcttc caggttgcag
     3961 ccgagttcgt aaaggatctc ggcggcggcg gccacgatgc cggctttgtc cggtccggcg
     4021 gcggtgagca ggtaataggg catgttttgg ttataacaca accgccgcgg catttcaaga
     4081 cgctgcaata ttctgtaaaa tggaagacgg acatggataa atctttcgtc gaccgattgc
     4141 gccgggccgt gggacgggga aacgttttaa cggatcccgc ctccctcctg gtttattctt
     4201 acgacgccgc caccgacaag gctctgccgg gcgcggtggt gaaacctcac gacacgaacg
     4261 aggtggcggc ggtggtgcgt ctggccgccg aggcgggggt tccttttgtg gcgcgcgggg
     4321 ccggcaccaa cctctgcggc ggttccatcc ccgccgacgg cgggctggtc atccatctct
     4381 ccggactcaa ccggatcctc cggatcgacc cgatcggccg ccgcgcgctc gtcgaggccg
     4441 gcgtggtgaa cctgcacctt cagaacgcgt tggcgccgca cggtcttttc tacgcccccg
     4501 acccggccag ccagaaggcc tgcaccctgg gcggcaacgc cgccaccaac gccggcgggc
     4561 cgcactgtct caaatacggc gtcacctcgc accacgtgct gggattggaa ctcgtgttgc
     4621 ccgacggccg cgtggtcaac acctccgtcg ccgacggcgg atatgacctc accgggctgt
     4681 tcatcgggtc cgagggcacg ctcggcatca tcacccggat ggaagtccgt cttctgcctc
     4741 tgcccgaggc ggtgcagacc ctgttggcgt cgttcccgtc cctggaggac gccgtccggg
     4801 ccgtgacggc catcgtttcg gcggggatcg tccccagcac gctggaggtg atggacggcg
     4861 tcaccctgcg ggcggtggag gcgttcgtcc acgccgggta cccggtggag gcgggggcgg
     4921 tgctgctcgt cgaactggac ggcccggcgg cgcggatcgc cgaggactcc gcgcgcgtcc
     4981 gggaatttgt tcgtcggaac cggggggggg acgtgcgcga ggcccgggac gagggggagc
     5041 gtcagaaact ctgggaaggc cgtcggggtt cgtatcccgc catggcgcgc ctggcgccca
     5101 acgtgctggt ggaggacggg gtggtgccgc gcacccgcct gcccgaggcg gtgcggcgga
     5161 tccgcgccgt ggccgaccgg gaaaacctcc gcatggggct catcgcccac gccggggacg
     5221 gcaacctgca tcccaacatc gttttcgacg agcgcgatcg cgcgcagacc gagcgggtcc
     5281 gcgccgccgg ccatgagatg atgcgcgtgt gcgtggagtt gggcgggtcc atctccggcg
     5341 aacacggcat cggcctgggc aagcgcgagg ccatgcgtcg gctttttact cccgaaacct
     5401 tggaggtgtt ccgcgctctc aaggacgcct tcgatccgcg ccgcctgtgc aatccggaca
     5461 agatcgtccc tcctccggcc gccgccggag aggaaaaatc agaggccccg tctcccgtgg
     5521 atttgccgga caacggcgtc ctctcggcgg agagcgtgcc gcgggtgcgg tctctgctgg
     5581 cgtcggcgtg cgcggcggga aggaaggtcg tcctgtgcgg gtcgggccgc tccgccgggc
     5641 cgacggacgg cgacgtcttg gccttgcgtt tggacggctt gacgggcgtg gtggaacacg
     5701 accgggacaa tttcacgttg acggtccggg cgggcatgcc gctgaaggac gtgcgcgcct
     5761 atttgacgcc gttcggacaa tatctccatc tttccggcga agggaccttg ggcggggtgc
     5821 tctcctcctg tcccgcgggc aaggagtcgg tgcgggacca gatcatcgga ctcaaagcgg
     5881 tgttggccga cggacggctc gtttcttttg gagggaaagt gatcaagaac gtggccgggt
     5941 acgacgccgc gaaactgttc atcggcgcct ggggaaccct gggcgcggtg gtggaggtga
     6001 cgctccggct ctttcccagc ccggcgctgg aaaaaattcc gtccgcgccc gcggaagatt
     6061 tttcttggat ggcctcgcct ctccatcgac gggtgaaaca ggctttcgat ccggcgggag
     6121 ttcttctgcc tccgcccggt cggggagcga ccaaatgaaa gacgccgaca agccggtcct
     6181 ggaacctcgt tgggcggaac tctcctccaa gaaagacgag gcgggctacg aggccgctct
     6241 ccactgcaac cggtgtgggt tctgcacctc gttctgcccg acgtatctgg ccacgggcga
     6301 cgaggggcgt tctccccgcg ggcgcaacca gaccttccgc gccctgctgg aaggacgcct
     6361 gaaagatccg gtgcagggcg gggacctgtt cgacacctgc cttctctgcg gcatctgcac
     6421 ctcggtgtgt tttgcggaag tgccgacggc caaactgatg tcggcggcgc gggagaaggt
     6481 gatgcagcgg gccggggagc cgtggctcat gcgggggctt cttcgctccc ttctgccgcg
     6541 tccccgattg ttcgagtttt tcctccaggt ccttttttgg gggaaacggc tcggcgtcag
     6601 ccgcgccttg aacaggctcg gggtgcttgg tctgttggac cgccgtttgg ccgccgccga
     6661 ggagctgacg gattccgtcc cgcgacggtt tctgcgggat ctcttgccga acgccccgcc
     6721 cgacgtggac gtcgtccaat tcgtggcctg cgggccgagc tacgcgatgc cggcggtggg
     6781 ggaggcgacg caccggctgg tgcgcgcggc gggaaaatcc gtcggtcggg tggacaacgt
     6841 ttgttgcggg ttgccggccc tgagtctggg cgattcggcg gcggcccggg ttttggcgga
     6901 aaagaacatc gccgaactgg aaaaatatcc gcgggccgtc gtcctggcgg acgactcgtc
     6961 ctgcgccgcc accctgaagg agtatccgtc cctttttgaa gagggggaat ggcgtcgtcg
     7021 cgccgaggcc gtgtcccggc ggacgaagga tctgagcgaa tggctggcgg agaaccggcc
     7081 gacggggact ccccttcctc cgacggtggt gacctaccat gacccgtgca aagcccgcta
     7141 cggccagaag atcgtggacg cgccgcgggc ccttctggcc gccacgcccg gggtggatta
     7201 ccgggaactg cccgaagccg accaatgttg cggcggcggg gggacctaca gttttctgca
     7261 gccggaaatt tcccgacagg tcctgtcccg gaagatccgg aacatcgaat ccaccggggc
     7321 ggcggtggtg ctgacctcgg ccgtttcctg cctgttgcag atccgcgccg gtctgaaatg
     7381 gaccggttct agggtgagag cgtttcattt gtcggagttc ctttcgcccc ctccagcgtc
     7441 cgatgagacg ggttttttcg ggcctaaaac gggcctttaa cccccgtttc aacttgttta
     7501 caatcaattt acatctcatt aatgagttag acccccctat agaagtatag tagtaacaga
     7561 gaacccaaac cgaccaaaag aattcaagcg cttgaatata atgaagcttt taagatataa
     7621 tttacgtagc cgttacaatc gtgggttctc taagttacgc ctgagggaga gggagccatg
     7681 accttcgatt gggcctatcg gtccaaatcc atcgcccgaa aatccgtcgc gctcttcatc
     7741 gtgacggcgt tcgtgtttac cacggtctcc gaaccctggg cgcaatcctc cttctgggaa
     7801 cagcagcgtt cctcccgcca aaacctctac aagcagaccg accctcagac cgccgaagcc
     7861 aaggcgctcc tctccagcgt ttcctggccc aaagaagcgg cgcacccgct gttcggttct
     7921 ttcaagctct cctcccaact ggggtcgatt ctcgagacgc atctggcggc ctcttccgag
     7981 ttggaaccgg aaaaaatccg acccctcacc ctctatcaca tccaggacgc gcacggcgtc
     8041 tacggcgccc aatacaacgc ctcccagatc atcgacgggc tggcggcggc tttcaaggag
     8101 caggaccagc ccaaagcccc tctgttggtc tgtcaggaag gcggactgaa gagcgtcaac
     8161 accgattggc tgtcttcctt cccgttcccg gagatcaagc gcgaggtggc ccacgcccat
     8221 cttcgcatgg ggaacctgac cggcgaggaa tacctggcgc tcgtccgctt gcccggcttt
     8281 gtgaagttgc gcggcgtgga gaacaaggac ctgtatttcg acaacctcga ttcccgccgc
     8341 aacaccattg aggcccgcct gcaggccagc aaatacatcg cttccctcca ggcgcgcatg
     8401 aacgtggtga aggagaacat ctacccgtcc tctctgctcg atctggaccg gaagttccgc
     8461 gcctactccg acaagaagat caccttcgtc caatacctcc aggaactggc ccgccggtcc
     8521 tcgtcgccga tcagccgggg ggaattcccc aacgttcacg ccaccctcct gctgtcggac
     8581 atggagtccg tcctcgacct cgacgcgctc cgggaggaac ggaccacgct ggtggacgtg
     8641 atggcggagc acctcagtct ggattccctc aaggaactgg tggccaaggc ctcggagatg
     8701 agactggggc acgtgaaccc ccgcgtgttc tatcagggcc tggtctccat cgccggcctg
     8761 ttgcggcaga aaggcgtggt cgttcccacc gacaacctcc gccaatacgt ggcctatctg
     8821 gaattgatgg aacgggtcaa ccacgagcaa cttcttttgg aaacggacca gctgaaaaaa
     8881 cgtctcctgg aacattacgc gtacaacgat cagatctggc ggttggtgaa cctggaccag
     8941 cggctccagc aggaagccga tctctgggga ttggccatgg ggccggatca gttcgccggc
     9001 tatcgcaagg aggggccgca ggatttcatc ggagtggaaa aatatctgtt ggcgcaggag
     9061 cgttccctcg gcctgtcccc ggtgccggtt tccgaacccg tcaacctgca gccctggttg
     9121 gagaagcggc cgctcatcca ccgctattac gaattcgccc tggaacgaaa cctggtgctg
     9181 gtgcagaacg cgttgaacgc catgaaggag acgggcgctt cccgcgcggt gctggtggcc
     9241 ggcgggttcc acacccaggg catgaccgat cttctgcggc agatgggtgt gaactacgtg
     9301 gtcatccagc cgcgtttcga ggcctatcag cccgtggccg aggaccagct cctccaaatc
     9361 agctacagct acgatttttc cgagtccgtc ggcagcgtcc gggagcagaa ccggctggac
     9421 atcacgggcc cgctcgccca ggacctctcc aagcagatcg ccctgcagga gagcatcgac
     9481 ggcgtggtca gcatggtgct ctcccggttc ggcaacgaac gggccgccaa cgccgaggcg
     9541 ctggaaaaat ggtgggccga ggcgcgcggg atcattccca tcgatgtccg ccgggcgatc
     9601 gaactggaga aggggaagtc ccggcaggtg gtcctcttcg gccgggccgc cggcgagccg
     9661 gtggcgctgg tctatgattt tcgcaacaag atcatggtcc tgatcggcga gaacagcgac
     9721 ctctccgacg cca
//
LOCUS       DAOTFD010000075         9581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5039_length_9581_cov_4.704073, whole genome shotgun sequence.
ACCESSION   DAOTFD010000075 DAOTFD010000000
VERSION     DAOTFD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5039_length_9581_cov_4.704073"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1458
                     /locus_tag="P5079_05990"
     CDS             118..1458
                     /locus_tag="P5079_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRY29573.1"
                     /translation="MVSALQRGDFSGTTDPGPDDALGHLGQALESLRRHVSQKENETV
                     TLWKLVEEVNTGRHPEEILDRIYESFHRLIPYNRIGASVLDDSGLSVTSFWARSDSPE
                     TKLPVGYSAPLEGSSLKGILETGRPRILNDLRAYLREHPRSHSTHLIVEEGMRSSLTC
                     PLAAQGKPLGFLFFSSREADAYRTAHVELFQFIAGQISLILEKGRTLAQVSELSELKS
                     RFLGIATHDLRHPLSVLLIYLAHMKNNGFGPIPEKALKYTELAEKSCERMLELINNLL
                     DVNAIIKGQLTLSPREVNIAEFLQGLHAANALLAESRKYKLTLAVEKDIPPVSLDPHR
                     IGQVIDNLLSNAMKYSPQNTTVAVAARKDGDELLIVVSDQGPGLSPEDISRLFVEYGR
                     GPTKAQRDEKSTGLGLAICKRIVEAHGGRIWAASRPGAGATFSFTLPLKPRSRK"
     gene            complement(1520..2137)
                     /locus_tag="P5079_05995"
     CDS             complement(1520..2137)
                     /locus_tag="P5079_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY29574.1"
                     /translation="MTWAWEGVMLGLATGPYCLTACAPMLVPYVLASVEGRRRRGLLT
                     LGEFLTGRFLAYVLFGAAAGFFSGRFSGTLPARGVAAAVFGAGVLMLLYSLPKTFPTV
                     RMCAHMNAFPGLRRLPLALGFLAGINICPPFLAGVARVLESGSAGFGGVYFAAFFAGT
                     SVYLLPLAAALPFTHIKRLQSIGTLACGVAGLWFMGTGVVGLLKG"
     gene            complement(2134..3285)
                     /locus_tag="P5079_06000"
     CDS             complement(2134..3285)
                     /locus_tag="P5079_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY29575.1"
                     /translation="MKRAENAKSFRGSFLLAFLLAAALSTAALWREEGEFLTAARLGW
                     ACFVGAIAFLIFYTGRISRWRALFFIVSAWAFVAYFKAFIFGLGNNAFFSPKIQEVPF
                     CHIAIASSFLNALYQQYLAFRSGSWGLWGPLSLGVLWLGVTLAIGQGWCSWACYYGGL
                     DEGFGRVLKKPLFRDFRLPERMRDLPAAVLLVMLFVSLSALLPVFCLWVCPLKVTTAF
                     LDTERATRLIQLAVFSISGALSLVLLPLALKKRVFCGLLCPFGAWQAFWGRINPFRVS
                     IDGDKCTGCGRCLKVCPTFVLNDGTLQNQTVSAYCNRCGECVDACSTGALSYTVWGRS
                     LPVRPGFPGELADVRVFFVMSALGVGGAVGGVYVPDLMSRIFSWLAGAP"
     gene            3384..5525
                     /locus_tag="P5079_06005"
     CDS             3384..5525
                     /locus_tag="P5079_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29576.1"
                     /translation="MDFLIFWIGLYSLLAQTILLRELAFLFPGNELALASTFAAWLFW
                     TAVGASHPPDPKTRTRFPLHLGLLALTVPATDLLIRLSRLFLPPGRPAGLFFTVTLPF
                     VTLAAAGWLFGKTFSANVVLAEKNGGRNAPGRTYFLEGLGALAGGGLSLLFFDLGNSS
                     LGVLSVSGATLAALNFFLFRRAAPKTTSLFLCLGLLFAAGARPLDNGTRRAQWRRYPL
                     VATRETPYAHAAVARWKADLLFFENGSISAQWPDPERYERLAHWPLLCHPNPRRILVL
                     GLPGALAAGEILKHPVDALDIVLTDKRAWDLFRGPLDGDDPGPRRDERIRFHFTDPRT
                     WIKEHPRTYDVILQSLPEPQNISGNRLFTEEFFSAARQGLEENGLLSFSLASSDNYLS
                     PETLFANASILRAVEKSFPFQELLPGAALTVLAANREILLDPQALAARHHQRRLETTA
                     LHPKTFEWTLQPERRNLLRLRLDDLRHVVPNSDLFPVAIFLTWRVWLTKFVSSPDLLG
                     VVAAMVLLALLLKKAARHWKDPATLPENSTLFAVGFAAMSLEIVLLYVFQAASGALYW
                     QIGILTAAFMGGLSAGSFCGFRRSGPPPNGAALGRATVLMAGLCALTAVELDRLPSFT
                     APLPAAMALLSAGGFLVGWTFSRASSGRTEKAGGFYSADLWGAALGVFLTGTFLSPLL
                     GHAGALFVSTGLLFLSLVVSKVFSALRKRRS"
     gene            complement(5488..6969)
                     /gene="amrB"
                     /locus_tag="P5079_06010"
     CDS             complement(5488..6969)
                     /gene="amrB"
                     /locus_tag="P5079_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY29577.1"
                     /translation="MKRIPAIFCLTVWGAGLWAGEARPPAVAGQFYPSSPGALKETVD
                     ALLSKAPSHELRGDVVALWVPHAGYVYSGAVAAEAFKCLSKEVRTVVLLGPSHHVWVK
                     SAAVYGEGRFKTPLGEVDVDEELAGRLLGLSPLFERLPSAHAREHSLEVQLPFLQRGL
                     SKFKILPILTNVEDPAQARRLGAALAEALRGRKAVLVISSDLSHYPTREVARRADETF
                     LRAAGRMDPEYLRLTSQVTLGRGEEQLQTTACGQGALMTGICAAKALGADSLRVLRYV
                     NSGEIPEIGDPNRAVGYAAAVFVNAGTAVPREFVLKPADKAALLSAARRSIGKALDGG
                     AYAPEAMAENAELNLPAAVFVTLQERGRLRGCIGTTTPQSSLWEGVRYFARAAAFDDP
                     RFPPVQKGELDELHIEISVLSVPERVKDASVVQPGRHGVIVRRKDRMGLFLPTVWEQL
                     PDKAMFLSELCSQKAGLPEDCWEDPEVELSVFTTDVFEEPKRP"
     gene            complement(6987..8231)
                     /gene="amrS"
                     /locus_tag="P5079_06015"
     CDS             complement(6987..8231)
                     /gene="amrS"
                     /locus_tag="P5079_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRY29578.1"
                     /translation="MTGTFHPPRHRTAATYLGCLAGLLTSLATLVLLSGFPPPGTFAA
                     LFPSTNPRVAEARYWRTACAGVQCGLCPFHCFLPEGARGRCRVRMNVGGRLKTLVQNS
                     PVSVHIDPIEKKPVFHLLPGTGIYSIATVGCNLACDFCQNWEISQAYPEQIKDGVVVP
                     REVRFAGRRQGRPTFELRQEAVQTMTPAEVVRAARDTGCRSIAYTYSEPVVFFEYVLE
                     TAKRAKENGLRNVMVSAGYISPGPLAELAPYLDVVKIDLKGFDEGYYRGTVGGELRFV
                     LRTLLELKKLGVLTEIVNLVVPTLNDKEEDFDRLSRWVRENLGEETPVFFSRFTPQYK
                     ATHLPATPLETLERAYASARRAGLKFVYLGNVPGHPAESTYCPRCGGALVRRHGYAVL
                     ENRIGKDGTCPDCHKKIPGRWG"
     gene            complement(8404..8988)
                     /locus_tag="P5079_06020"
     CDS             complement(8404..8988)
                     /locus_tag="P5079_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY29579.1"
                     /translation="MRMKQLAVILFLAGFLGTARAADTYKIDPAHSSIGFSVKHLVIS
                     TVKGNFTDYAGTILFNEKDVTKSSVKVVIKSASINTGIAARDNHLRSPDFFATEKYPE
                     LTFESKRVEKRPEGYAAMGTLTMRGVSKEVVLPFTLSGPITDPWGKSRIAAESGLTVN
                     RQDYGVSWSQKLDNGGLVVSDEVKIELAVEAVKE"
     gene            complement(9070..>9581)
                     /locus_tag="P5079_06025"
     CDS             complement(9070..>9581)
                     /locus_tag="P5079_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29580.1"
                     /translation="FGQALRTATRGYQSVAVPGTQTLEIRAPGFYTGLYLHKDPGPMP
                     VEEIARLAVSIHDIEGGVYLPVEAAPPGQVFRRGSQTGMLLFQCEFPQKGRYLLDAVY
                     PADVAGPKAEILLLHEALQNNRSDIAAGIIFCLVFCGAGTYVMLRARRQAARDRESAQ
                     VPSVKKKQR"
BASE COUNT         1907 a         3350 c         2658 g         1666 t
ORIGIN      
        1 tgatgtcggc cctcgcccgc gccgttgacc cctctcctga aaacgtcggc cgtggcgagg
       61 gaagaacgcg cttgatatac taatcccatg gaccctcgga ttccaaaata catcgacatg
      121 gtatcggccc tgcagcgggg cgatttttcc gggacgacag accccggccc ggacgacgcg
      181 ctcggacacc tcggccaggc cctggaatcc ctccgccggc atgtgtcgca aaaggaaaat
      241 gagaccgtga ccctctggaa actcgtggag gaggtcaaca ccggccgcca tccggaagaa
      301 atcctcgaca gaatctacga atcgttccac cgcctcatcc cctacaaccg catcggagcg
      361 tccgttctgg acgactccgg gctgagcgtg acatccttct gggcgcgttc cgactcgccg
      421 gagaccaaac tccccgtggg atactccgcg ccgttggagg gcagcagcct caaaggcatc
      481 ctcgaaacgg gccggccgcg catcctcaac gatctccgcg cctacctgcg ggaacacccc
      541 cggtcgcact ccacccattt gatcgtcgaa gaggggatgc gttccagcct cacctgcccc
      601 ctcgcggccc agggcaagcc cctcgggttc ctttttttct caagccggga agcggacgcc
      661 tatcgcacgg cccacgtgga gctcttccag ttcatcgccg gtcagatttc cctcatcctt
      721 gaaaaaggga ggaccctcgc ccaggtgtcc gaactgagcg agctgaaaag ccgcttcctc
      781 ggcatcgcca cgcacgacct gcgccacccc ttgagcgtcc tgctcatcta cctggcccac
      841 atgaagaaca acggattcgg tcccatcccg gaaaaagcgc tgaagtacac ggagctggcg
      901 gaaaaatcct gcgaacggat gttggaactc atcaacaacc tcctcgacgt caacgccatc
      961 atcaagggcc agttgacgct gtccccgcgc gaagtgaaca tcgccgaatt cctccaggga
     1021 ctccacgccg ccaacgccct cctcgccgaa tcacgaaaat acaaactgac cctcgccgtg
     1081 gaaaaagaca tcccgcccgt ctccctggac ccgcaccgca tcggccaggt gatcgacaac
     1141 ctcctgtcga acgccatgaa atattccccg cagaacacca ccgtcgccgt ggccgcccgg
     1201 aaagacgggg acgagctcct catcgtcgtt tcggaccagg ggcccggact ctcgcccgag
     1261 gacatctccc gccttttcgt ggaatacggc cggggaccga ccaaagctca gcgcgacgaa
     1321 aaaagcaccg gtttggggct ggccatctgc aaacgcatcg tggaggccca cggcgggcgg
     1381 atctgggcgg cgagccggcc cggcgccggc gccacctttt ctttcaccct ccctctgaag
     1441 ccccgttccc gaaaataaaa atcccgcctt tcggcggggt ccggtgattt caacgccggc
     1501 gcggcgcgaa cggacgatgt caccccttca ggagtcccac cacccccgtt cccatgaacc
     1561 acagccccgc cacgccgcaa gccaacgtcc cgatggactg cagccgtttg atgtgcgtga
     1621 acgggagggc cgccgccagc ggcaaaagat acacgctggt cccggcgaaa aatgccgcga
     1681 aatagacccc gccgaatccg gccgatccgg attccagcac gcgcgccacg ccggccagga
     1741 acggcggaca gatgtttatc cccgccaaaa aacccagcgc cagcgggaga cgacgaagcc
     1801 cgggaaacgc gttcatgtgc gcgcacattc gaacggtggg gaacgttttc gggagggaat
     1861 acaaaagcat caagacgccc gccccgaaaa cggccgccgc cacgccgcgg gcggggagcg
     1921 tccctgaaaa acggccgctg aaaaatcccg cggcggcgcc gaaaaggacg taggccaaaa
     1981 aacggcccgt caaaaattcg ccgagggtga gcagccctct ccgccggcgc ccctcgacgg
     2041 acgccaacac gtacggcacc aacatcggcg cgcaggccgt caggcagtaa ggacccgtcg
     2101 ccaaccccaa catgaccccc tcccacgccc aagtcacgga gcgcccgcca gccatgaaaa
     2161 aatccgagac atcagatccg gcacatacac gcctcccacc gccccaccca ccccgagcgc
     2221 cgacatcacg aaaaacacgc gcacgtccgc cagttccccc ggaaaacccg gacggacggg
     2281 caacgaccgc ccccagacgg tgtaggaaag ggcgcccgtc gaacacgcat cgacgcattc
     2341 cccgcagcga ttgcaatagg cgctgaccgt ctggttttga agcgtgccgt cgttcaacac
     2401 gaaggtcgga cagactttca ggcatcggcc gcagcccgtg catttgtcgc cgtcgatgct
     2461 gacgcgaaac ggattgatcc ttccccaaaa agcctgccag gccccgaacg ggcagagcag
     2521 cccgcaaaaa acgcgtttct tcagggccag cggcaacagg accagactca acgctccgga
     2581 aatggaaaaa accgccagct gaatgagccg cgtcgccctc tccgtatcca agaaagccgt
     2641 cgtcaccttc aacgggcaga cccaaagaca gaacaccggc aacaaggcgc tcagcgaaac
     2701 gaaaagcatc accaacaaca cggcggccgg gaggtcccgc atcctctccg gaagacggaa
     2761 atcccggaac aacggctttt tcaaaacacg gccgaatccc tcatccaggc cgccgtaata
     2821 gcaggcccag gaacaccatc cctgtccgat ggcgagcgtc acccccagcc agaggacgcc
     2881 caaagagagc ggcccccaca atccccacga accgcttcga aacgccagat actgctgata
     2941 gagcgcgttg aggaaggacg aagcgatggc gatatgacaa aacggcacct cctgaatctt
     3001 cggagagaaa aaagcgttgt tccccaaccc gaaaatgaac gccttgaaat acgccacgaa
     3061 ggcccaggcg gaaacgatga agaagagcgc gcgccaccgc gatatccggc cggtataaaa
     3121 gatcaggaaa gcgatcgcgc ccacaaaaca cgcccatccc aaccgggccg ccgtcaggaa
     3181 ttccccttcc tcacgccaca gcgccgccgt ggaaagcgcc gcggccagca ggaaggccaa
     3241 caaaaagctg ccccggaacg atttcgcgtt ctccgccctt ttcatctttc agcttctcca
     3301 aaaacagctc ataccgcctt cattttagaa aaaaaccggc cgccgcactg gaacgccgcc
     3361 cccgtttgga ctaaaatgat tttatggatt tcctcatctt ctggatcggt ctttattccc
     3421 tgcttgcgca aacgattctc ctgcgggaat tggcgttcct ttttcccgga aacgagctgg
     3481 ctctggcgtc gacgttcgcc gcatggcttt tctggacggc ggtcggggcg tcccaccccc
     3541 cggaccccaa aaccaggaca cggttcccgc tccacctggg cctcttggcg ctcaccgttc
     3601 cggcgacgga ccttttgata cggctctccc ggctcttcct gccgccgggc cgtccggccg
     3661 gcctgttttt caccgtcacc ctgcccttcg ttacgctggc ggccgcgggc tggctttttg
     3721 ggaaaacttt ctcggccaac gtcgtcctcg ccgaaaaaaa cggggggcga aacgcgccgg
     3781 gacggaccta ttttctggag gggctcgggg cgttggccgg ggggggcctc tccctgcttt
     3841 tcttcgacct gggaaattcc tctctcggtg tcctttcggt ttccggagcg acattggccg
     3901 ccttgaattt tttcctcttc cgccgagccg cccccaaaac gacgtccctc ttcctgtgcc
     3961 tcggacttct cttcgcggcc ggcgcccggc cgctggacaa cgggacccga cgcgctcagt
     4021 ggaggagata tccgcttgtg gcgacgcggg aaacgcccta cgcccacgcc gccgtcgccc
     4081 gctggaaagc ggacctgctc ttcttcgaaa acgggtccat ctcagcccag tggcccgacc
     4141 ccgaacgata cgaacggctg gctcattggc cgctcctctg tcatccgaac ccgcgccgca
     4201 tcctggtcct cggcctgccg ggggcgttgg ccgccggaga aatactcaaa caccccgttg
     4261 acgcgctgga catcgttctg acggacaagc gcgcgtggga cctcttccgc ggaccgctcg
     4321 acggcgacga tcccggcccc cggcgggatg aacggatccg gttccatttc accgacccgc
     4381 ggacctggat caaggaacat ccccgcacct acgacgtcat ccttcaatcc ctgcccgaac
     4441 cgcagaacat ctccggcaac cggctcttca ccgaagaatt tttcagtgcc gcgcggcaag
     4501 gattagagga gaacggactg ctttcgttct ccctggcctc ctctgacaat tatctctctc
     4561 cggagaccct cttcgccaac gccagcatcc ttcgggccgt cgaaaaatcc ttcccgtttc
     4621 aggaactcct ccccggcgcc gccttgacgg tcctggcggc gaaccgggaa attctcctgg
     4681 atccgcaggc cctggcggcc cgacatcatc agcgacggct tgaaacaacg gccttgcatc
     4741 ccaaaacgtt cgaatggacg cttcaaccgg aacgccgaaa cctcctccga ctccgactgg
     4801 acgacctccg gcacgtcgtc cccaacagcg accttttccc cgtcgccatc ttcctcacgt
     4861 ggcgcgtctg gctgaccaag ttcgtttcgt ccccggacct tttgggggtg gtggcggcga
     4921 tggtcctgtt ggcgctcctt ttaaaaaaag ccgctcgcca ctggaaggac cccgccacct
     4981 tgccggaaaa ttcaacgctc ttcgccgtgg ggttcgccgc catgtccctg gaaatcgtcc
     5041 tcctctatgt ttttcaggcc gcctccggcg cgctctattg gcagatcgga attctgacgg
     5101 cggctttcat gggcgggctg agcgcgggca gtttctgcgg cttccggcgg agcgggcccc
     5161 ctccgaacgg cgcggcgctg ggccgggcga ccgttcttat ggcgggactc tgcgccttga
     5221 ccgccgtcga actggaccgg ctcccgtcgt tcacggcgcc gctaccggcg gcgatggctc
     5281 tcctgtcggc cggcggattc ttggtcgggt ggactttttc gcgggcctcc agcggaagaa
     5341 cagaaaaagc cggcgggttc tattccgccg acctctgggg cgccgccctg ggcgtttttc
     5401 tgacggggac attcctctcc cctctcctgg gacacgcggg ggcgctcttc gtgtcgacgg
     5461 gacttctttt cctatcgctc gtcgtctcta aggtcttttc ggctcttcga aaacgtcggt
     5521 cgtaaaaacg ctcagctcca cctccgggtc ctcccagcaa tcctccggaa gtcccgcctt
     5581 ctgcgagcac aattcggaca ggaacatcgc cttgtccggc aactgttccc aaaccgtcgg
     5641 caaaaacagg cccatccggt cctttcgccg cacgatcacc ccgtgccgtc ccggctgaac
     5701 aacggaggcg tccttcactc tctccggcac ggacaaaacg gatatctcga tgtgcagctc
     5761 gtccagttcc cctttctgca ccggcgggaa acgcgggtca tcgaaggcgg cggcccgggc
     5821 gaaataccgg accccctccc acaggctgga ttgcggggtc gtggtgccga tacatccccg
     5881 caaccggccc cgttcctgga gggtgacgaa caccgcggcc ggaaggttca actccgcgtt
     5941 ctccgccatc gcctccggcg cgtaggcgcc gccgtcgaga gctttcccga tggaccgtcg
     6001 agccgccgac agcagggccg ccttgtcggc cggtttcaaa acaaactccc gcggaacggc
     6061 ggtccccgcg ttcacaaaaa ccgcggcggc gtatcccacc gcgcggttcg ggtcgccgat
     6121 ctcagggatt tcgccggaat tgacgtatcg cagcacccgc aggctgtcgg cccccagggc
     6181 cttggccgcg caaatccccg tcatgagcgc cccctgtccg caggccgtgg tttgcaattg
     6241 ctcctcgccg cgccccaacg tgacctgaga cgtgagccgg agatattccg gatccatccg
     6301 ccccgccgct cgcaaaaacg tctcgtccgc ccgccgggcc acctccctcg tcggatagtg
     6361 cgacaaatcg gaagagatga cgaggacggc tttccggccg cgcaacgcct cggcgagggc
     6421 cgcccccagc cgtcgcgcct gcgcggggtc ctcgacattc gtcagaatcg gaagtatctt
     6481 gaatttcgac aatcctcgct ggaggaacgg gagctgcact tcgagggaat gctcccgcgc
     6541 gtgcgccgaa ggcagcctct cgaacagagg cgacaggcca agcagtcggc cggccagttc
     6601 ctcgtccacg tccacctcgc cgagcggcgt tttgaaacgc ccttcgccgt agaccgccgc
     6661 cgatttgacc caaacatggt gagacggacc gagaaggaca acggtccgga cctctttgct
     6721 caggcatttg aaagcttccg ccgccaccgc gccggaataa acgtatccgg cgtgcggcac
     6781 ccacaaggcg acgacgtcgc ccctcaactc gtgcgacggc gccttcgaga gaagtgcgtc
     6841 gaccgtttcc ttcaaggcgc cgggagagga cggatagaac tgccccgcca ccgcgggagg
     6901 acgcgcctcc cccgcccaca aacccgcccc ccacaccgtc aaacaaaaaa tcgccgggat
     6961 ccttttcatc gtctcccctc cttttctcaa ccccatcgac cgggtatttt cttgtgacag
     7021 tccgggcatg tcccgtcttt tccgatccgg ttctccagaa ccgcatagcc gtgccgccgc
     7081 acaagggctc ctccacaccg gggacaatag gtggattcgg ccggatggcc gggcacattt
     7141 cccagataga cgaatttcag tccggcgcgg cgggccgagg cgtaggcccg ctccagggtc
     7201 tccagcggag tcgccggcag atgggtggcc ttgtattgcg gggtgaatct ggaaaagaac
     7261 accggcgtct cttccccgag gttctcccgg acccaccggg aaagccggtc gaaatcctcc
     7321 tctttatcgt tcaacgtcgg caccaccagg ttgacgatct ccgtcagcac gccgagtttt
     7381 ttcaattcca acaacgtccg caagacaaac cgaagctctc cccccaccgt cccccggtaa
     7441 tatccctcat cgaaaccctt caggtcgatt ttaaccacgt cgagataggg cgcgagctcg
     7501 gccaacggtc ccggactgat gtatccggcc gataccatca cgttccgcaa cccgttctct
     7561 ttggcccgct tggcggtttc caggacatat tcaaagaaaa cgaccggctc cgaatacgtg
     7621 taggcgatgg aacgacaccc cgtgtcccgc gcggcccgga cgacctccgc cggggtcatc
     7681 gtctggaccg cctcctgccg caactcgaac gtcggccggc cctgcctccg ccccgcgaac
     7741 cgcacctccc tcggcaccac gaccccgtct ttgatctgtt ccggataagc ctgagaaatt
     7801 tcccaattct ggcagaaatc gcaggcgagg ttgcagccga cggtggcgat ggaataaatg
     7861 cccgttcccg gcaagagatg gaacacgggt ttcttttcga tggggtcgat gtggacggac
     7921 acggggctgt tctggacgag ggttttcaac cggccgccga cgttcatccg gacgcggcaa
     7981 cggccccggg ccccttccgg caaaaaacaa tggaacggac acaggccgca ttggacaccg
     8041 gcgcaggcgg ttctccaata acgtgcctcc gccacgcggg gattcgttga gggaaacagg
     8101 gcggcaaacg tccccggagg tggaaatccg gagagcaaga ccaacgtcgc cagagacgtc
     8161 aaaaggcccg ccagacaccc caggtaggtc gccgcggtgc gatggcgcgg cggatggaag
     8221 gttcccgtca ttttttcggt tctccgaccg gcgagagcgt acgggggaaa tttcgtttct
     8281 ccctgcccat actatatcac atccccggcc tctcccccgg ccggccccgt tgacgggcca
     8341 tcgcccgcaa acgaaaaggc cggcgacgga tgtcgccggc ctgtctgact ttttatccgc
     8401 ccgtcactcc ttgacggctt ccaccgccag ctcgatcttc acctcatcac tgaccaccag
     8461 gccgccgttg tccagcttct ggctccagga aactccatag tcctgccggt tgaccgtcaa
     8521 ccccgattcg gcggcgatgc gcgacttgcc ccaggggtcc gtgatgggcc cggacagggt
     8581 gaacggcagc accacttctt tcgaaacccc ccgcatcgtc agcgtgccca tcgccgcata
     8641 cccctcgggc cgcttttcca cccgcttgct ctcgaacgtc agttcgggat acttctccgt
     8701 ggcgaagaaa tcgggcgagc ggagatggtt gtcgcgggcc gcgatgccgg tgttgatgct
     8761 ggccgacttg atgacgactt tcacggagga tttcgtgacg tccttctcat tgaaaaggat
     8821 cgtgcccgca taatccgtga agttcccctt gaccgtggat atgaccaaat gtttcacgga
     8881 gaagccaatg gacgaatggg ccggatcgat cttataggtg tcggcggccc gggccgttcc
     8941 caaaaatccc gccaaaaaca aaatcaccgc cagctgtttc atacgcatga acgcatctcc
     9001 tttgaccttc ctttccttca ccgattaaaa cgccctcttc cgggaaaagt tcccggaacg
     9061 ggaacttcgt caacgctgtt tcttcttgac cgacggcact tgagccgact cccggtcgcg
     9121 cgccgcctgc cggcgggcgc gaagcatcac atacgtcccc gccccgcaga acacaagaca
     9181 gaagatgatc ccggccgcga tgtccgacct gttgttttga agcgcctcgt gcaacaggag
     9241 gatctccgcc ttggggccgg ccacgtcggc cgggtagacg gcgtccaaca gataccggcc
     9301 cttctggggg aattcgcact ggaacaagag cattccggtc tggctccctc gtcggaaaac
     9361 ctgccccgga ggggccgcct cgacgggcag atagacaccg ccctcgatat catggatcga
     9421 caccgccagg cgggcgatct cttcaacagg catggggccg ggatccttgt gcagatacaa
     9481 gccggtgtaa aaaccggggg cgcggatctc cagggtctgc gtgcccggca ccgcgaccga
     9541 ttgataaccg cgcgtcgcgg tgcgcagcgc ctgcccgaac g
//
LOCUS       DAOTFD010000076         9544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5065_length_9544_cov_5.061123, whole genome shotgun sequence.
ACCESSION   DAOTFD010000076 DAOTFD010000000
VERSION     DAOTFD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_9544_cov_5.061123"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="P5079_06030"
     CDS             <1..1838
                     /locus_tag="P5079_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29581.1"
                     /translation="CQRTWTVNEVALAAAYAGEAEELKIRKEALEKQVADTERTLREE
                     TILGQERANVLSAEIEEHREKIEAQKHEAEKAFREKQSRFMEEISALTQSVRRIESDT
                     ALSLRLQEEKKKALEEDVSAARRVWAQKHKEKSEQWLPVIGRLRGEVELLEKTFAGEQ
                     ARFEAEKQKAQETLAALEKEEAHWREKTAAVQAEREKQEKEERRRFDEQCGKLSEELR
                     AKQQEASDRLKAREEELQRLNAMLVEEEQRESGAVQQEAARYQGEVSELEGKVRDLQL
                     RLRTQESEFADKLAPLQKRRSEIEMELAMKSASLKSEELRFQREAEKNRRPLEQKVRE
                     LERKLAEEEGSLQSQVKAKESLLKTLQTRLQLRLDRSEAERNRRAQQTDLVRQDLSQA
                     FQQLRARLEAGEQQQRQELETRVRALAELERALQKQKDMARETKEAVQSQMEQEKQAL
                     EMMCADLERRFSEKRETWERLLAEKDAQVRGMVEELESSDRNLQRENELRQRWTGWTN
                     EAVAQIRQMAAQTLDRLDDRKGHLGELLQKGIDAYSQGRHVDALTILARCIEIEPTYE
                     SAWQYRVLCLEALGMADQAREAARMVLQINPGNDIFKKWLEKTDK"
     gene            1878..4883
                     /locus_tag="P5079_06035"
     CDS             1878..4883
                     /locus_tag="P5079_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29582.1"
                     /translation="MVEDKSKDPKLPPPPGGLPRPPLPPLPGGGAPRSPLGAPPRPPL
                     PMAPTAPPAPAVSEERFREMESRSKELEETRKTLEAKLADMEKRLTEEHEKVMTAAMR
                     SKEEAALSAKVENSLKDIQERLRREKREQEMESARVAAEARIAELERRMAEERETWVA
                     TLKNQMAQRDVESRQIEDTFSTRLVEFERKWSDEKSRLEQAVQQKAEEVEKLKQTHAL
                     EMERTLNEWEKRLSTALSEREAFAQQMAELQKRAEQERALFQSAREEREKEMILLKAN
                     YALAENQRKSDLERLQKELAALSEDRNLARQEAVKYSEELQRLREQSAAAEKSQTEAQ
                     AQNVIALEGLKKELSDTRSAREHLQREAEDRARQIQELKDGLSAVENVRAGLVAQNEA
                     LRREQEQAGQQVFRLEQTVASKEKEMESLRNASAALQSRVVESEKELEGKNQRLEFLR
                     GETAAKEALQAKLQEEMQMKHSLQTSLSRAENDLSEAQRFVRTLEERLLAEKNELSKL
                     REEAALWRSTREQAEDKLRQAEQRAGSAVLRQQELESQLGEKERLLKSAQEEVRRLAE
                     RLSSMDNDRRVNEERLSELRLEMAERLGEARTRFDAQVAQSQEAVRTLEGELAAAIQQ
                     REGFQRDWQKAADDARRWNQQLMEQESQRKLLEDRLAQLRQELIQRGEEARSRFEPQL
                     TQAQEVIRTLQDNVSKLMQEKESYERQARKAEEEVARSSQRVQELEAEKRLTDERLNR
                     LREETATLEITLRDKIESEYRGLAERAEMEQRQVHQWAQETVRSAEQKLREQASRGQQ
                     KIQALQSSMAEDLAAFDKELAALRAERTQLVHERDSVVESLRKSDEKFQESYQRWDQE
                     RALLRESLKEAEKSLQELRSRAESDRRAWEEDFQRREKSSTETISTQWEERVKMLQAH
                     VEDERRLWTEKLKTLDENVARLEGEKRRFLDQRNEEISALRKQYENELENLRQALMRR
                     PRGLFGSVGAWLNQPVIEVNLGARDKRRE"
     gene            4880..6592
                     /locus_tag="P5079_06040"
     CDS             4880..6592
                     /locus_tag="P5079_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29583.1"
                     /translation="MTMSLSMEHIRQKWLKRLGEAAQDFGSAEEDLSEVLMPDMEPPP
                     KPTPILFRRSHGGVLPRETGHLEEGFHALRESLAQTKLQRQQAETELEKIRKDYVAAD
                     GRINELAMNLAALEEKKAQLEKSLASLQDALRTRDEENTVLRGETAHLQATIAGKDKS
                     LEEMRALQKELEAQLEELRAHMAGSAKESERAYAEAERRVAESAAVIREKTARIEELE
                     RELSTDSEESRQDFQDALEKKETEIRLLSENIKRLSQQNKDMLAALQKFQTSGGLADP
                     SGMAPAAEPDAAAREKIESLELALASRDRMMTDQEKLIADKQKTIEELFRKVRELDAC
                     AAGRPADSSETANLTKQVELLLEENKKWRKEAEEKAGFSRETSMRAESLAAENAALSD
                     RLKAAESSSADRASLESKLADQEKLFERKFEDWQVRISEKERRLGEVEKAKVELEAEL
                     KARRDEFEKNVRLCDELRMKNEELETQLRLLVMNSEAIDRMEKNLRETFRLMTPGGTT
                     FPIPRPRDEQPAEQRPGFFGRLLNWWRAPLVKIPKKSSTAAPAPAVQRIQQNLKARRN
                     VKPQ"
     gene            6589..8238
                     /locus_tag="P5079_06045"
     CDS             6589..8238
                     /locus_tag="P5079_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29584.1"
                     /translation="MSREPSPSLLKRIWDRILSWLGLADDAQAGAAAAEQERLTLIVQ
                     QHEAELLRLRRLLSDTQSSLQRSEQERNKSLDTLNDREIRLEKEKSRAAVAGVLIKDY
                     DIELKKIRREKLTQQRIFEDKIHALLEKLNELQRSLPTAPSANLPSPVVAKSLALEKQ
                     ALQQELHETQEKLALLEDKLDRETSARLDVQKQSASLQSGSAELQEKFRAAESALWNE
                     KTKTEALEKEKTVLQKQVMELQKTLEAVKNVDSVQEPASVSQEVIALRERVQALDEAL
                     RSARAAAAGGGGGPAPVSDEELSPIPHGVQMELDTLRSALEQERARWEEERLRTEAAL
                     EEEKRRADKETRLRRTLEERTTALSRETEKWRSTHEEARKQWEDERKRLLEQWDKEKE
                     HRLEELEMHRQAEDRAGSLLEEIETLRKTLEKERASWANERTDLQQRQERDRREWGER
                     LDRAAKLRGDPGELEEIRKGFEQSRAVWEKERGDLTARWEKEREEWRERLEAAAGSSS
                     AAGGFTATQEEVDRLLGELDALTAENAFLKDQLGWEESKPS"
     gene            8277..9404
                     /locus_tag="P5079_06050"
     CDS             8277..9404
                     /locus_tag="P5079_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29585.1"
                     /translation="MTNPPRPQLNISAKLDTRLRGTLVARVQTAQLLKMGEPDIAKLI
                     KEIESDPLFLKMLYPPKPDWKIVRFQPHPRTSLSHAFYELNDQLLPQDTPADVVQLLE
                     NRRGLREKIRGMGPEAFERYFLRADENLSPEEAGEACGLTPEEVRQVQDFLLEFSVHA
                     EFFDRSSKSVPGIRVVRLGRVQMTDDDVFIEWNSPHLARGRYEIQYDRMEELIRKGLL
                     APEEKRHLKALIHRLELVNWRQSALYRIVYLLCYTQRDYLAQRDTMKRRSITQRRMAR
                     QLSVAPSTVNRAVQGRSLILPWGEELLLEELFCSRKELCLDALDALDAADKDFKDRTD
                     NELRERLKKSLGFTVPRRTVNTYRRQLQSRSPSSSGSGEVP"
BASE COUNT         2184 a         2596 c         3389 g         1375 t
ORIGIN      
        1 actgtcagcg cacgtggacg gtcaacgagg tggcgctggc ggccgcctac gcgggggaag
       61 ccgaagaatt gaaaatccgc aaggaggcgt tggaaaaaca ggtcgccgac accgaaagga
      121 ctttgcgcga ggagacgatc ctgggacagg agcgggccaa cgtcctgtcg gccgagatcg
      181 aggaacatcg agaaaagatc gaggcccaga aacacgaggc ggagaaagct ttccgggaga
      241 aacaatccag attcatggag gagatctcgg cgttgacgca gagcgtgcgc cgcattgagt
      301 cggacacggc gttgagcctg cggttgcagg aggagaagaa aaaagccctg gaggaggatg
      361 tgtccgccgc ccgccgggtc tgggcgcaga aacacaagga gaagtcggaa cagtggctgc
      421 ccgtcatcgg ccggctgcgc ggagaggtcg aacttctgga gaagaccttc gccggcgaac
      481 aggcgcgttt tgaggcggaa aaacagaaag cgcaggagac tttggcggcg ctggaaaagg
      541 aggaggcgca ctggcgggaa aagacggccg ccgtgcaggc cgaacgggag aaacaggaga
      601 aggaggagcg gcgtcgtttc gacgagcagt gtgggaaatt gtccgaggag ctccgcgcca
      661 aacagcagga ggcctccgac cggttgaagg cccgcgaaga ggagctccag cggttgaacg
      721 cgatgctggt ggaggaggaa cagcgggaat cgggcgcggt tcagcaggag gccgcccgtt
      781 atcagggaga ggtgtcggaa ctggagggga aggtgcggga ccttcagctg cggttgcgga
      841 cgcaggaatc cgaatttgcc gacaagctgg cccccctgca gaagcgccgc agcgagatcg
      901 agatggagct ggccatgaag tcggcttcct tgaaatccga ggaactccgc tttcagcgtg
      961 aggccgaaaa aaaccgtcgt cctctggaac agaaggtgcg ggagttggag cggaagctgg
     1021 ccgaagagga gggcagcctt cagtcccagg tgaaggccaa ggagagcctg ctcaaaaccc
     1081 tgcagacgcg gttgcagttg cggttggatc gcagcgaggc cgaacggaac cgccgcgctc
     1141 agcagacgga cctggtccgc caggaccttt cacaggcatt ccagcaattg agggcgcgtt
     1201 tggaagcggg cgagcaacag caacgccagg agttggagac ccgggtccgc gccctggcgg
     1261 aattggaacg ggccctgcag aaacagaagg acatggcgcg cgaaaccaag gaggccgtcc
     1321 aatcgcagat ggagcaggag aaacaagccc tggaaatgat gtgcgccgat ctggaacggc
     1381 ggttctcgga aaaacgggag acctgggaac gacttttggc ggagaaggac gcgcaggtgc
     1441 gggggatggt ggaggagttg gagtcgtcgg acaggaacct gcagagggaa aacgaactgc
     1501 gccagagatg gacggggtgg accaacgagg ccgtggcgca gatacgccag atggccgccc
     1561 agacgttgga tcggttggac gacaggaaag gccatctggg agaacttttg caaaaaggga
     1621 tcgacgccta ttcgcagggg cggcatgtgg acgcgttgac gatcttggcc cgttgcattg
     1681 agatagaacc gacgtacgag tcggcttggc agtaccgcgt cctgtgcctg gaggcgctgg
     1741 ggatggcgga tcaggcgcgg gaggccgccc ggatggtgct gcagatcaac ccgggcaacg
     1801 atattttcaa aaagtggctg gagaaaacag acaagtagat ttaaacttgc tttgccgaga
     1861 aaaaaaggag cttagccatg gttgaagaca aatcgaagga cccgaagttg ccgccaccgc
     1921 cgggaggact gccgcgtcct cccttgccgc cgcttccggg cggcggcgcc ccgcggtcgc
     1981 cgttgggggc acctccccgg ccgccgttgc cgatggcccc gacggctccc ccggccccgg
     2041 ccgtttcgga ggagcggttc cgggagatgg aatcgcggtc caaagaactc gaagagacgc
     2101 gcaaaacgct ggaggctaag ctggcggaca tggagaagcg tctcaccgag gagcacgaaa
     2161 aggtgatgac ggcggccatg cggagcaagg aggaggccgc cctctccgcc aaggtggaaa
     2221 acagtctgaa ggacatccag gaacggctgc gccgcgaaaa acgcgagcag gagatggagt
     2281 ccgcccgggt tgcggcggag gcgcgcatcg ccgaactgga acgacgcatg gccgaggaac
     2341 gcgagacctg ggtcgccacc ctgaagaacc aaatggccca gcgcgacgtg gagtcccggc
     2401 agatcgagga cacgttctcc acgcggttgg tggagttcga gaggaaatgg tccgacgaga
     2461 agagccgtct tgagcaggcc gttcagcaaa aagccgagga agtcgaaaag ctgaaacaga
     2521 cccacgccct ggaaatggaa cggacgctca acgaatggga gaagcgcctc tcgacagcgc
     2581 tgtcggagcg ggaggcgttc gcccagcaga tggccgaact tcagaaacgc gcggaacagg
     2641 aacgtgcgct gtttcagtcg gctcgggagg aacgggagaa ggagatgatc ctgctgaagg
     2701 cgaactacgc cttggcggaa aatcaacgca agtccgatct ggaaaggctg cagaaggagc
     2761 tggcggcgct ttcggaagat cggaacctgg cccgtcagga ggccgtgaag tattccgagg
     2821 agcttcagcg gctgagagaa caatcggcgg cggcggagaa gagccagacc gaggcccagg
     2881 cccagaacgt catcgccctg gaggggctca aaaaggaact ctcggacacg cggtccgctc
     2941 gggagcatct ccaacgcgag gcggaggacc gcgcccggca gatacaggag ctgaaggacg
     3001 gattgtccgc cgttgaaaac gtccgcgccg ggctcgtggc tcagaacgaa gccctgcggc
     3061 gtgaacagga acaggccggc cagcaggtgt tccggttgga acagaccgtc gcgtcgaaag
     3121 agaaggagat ggagtccctg cggaacgcct cggcggcgct gcagtcgcgc gtcgtggagt
     3181 cggaaaagga actggagggg aaaaaccaac ggctggagtt cttgcgcggc gagaccgcgg
     3241 cgaaagaggc gctccaggcg aagcttcagg aagagatgca gatgaaacat tcgctccaga
     3301 cctcgttgtc ccgcgcggag aacgatcttt ccgaggccca gcggttcgtc cgcaccttgg
     3361 aagagcgtct cttggcggaa aagaacgaat tgtccaagtt gagggaggag gcggcgttgt
     3421 ggaggtcgac gcgggaacag gcggaggata aattgcgcca ggccgaacag cgggccggca
     3481 gcgcggtcct ccggcagcag gaactggaaa gtcagttggg ggagaaggag cggctcttga
     3541 agtccgcgca ggaggaggtc cggcgtttgg cggaacggct gtcctccatg gacaacgacc
     3601 gccgcgtcaa cgaggaacgt ctctcggagc ttcgcctgga aatggccgag cgcctcgggg
     3661 aggcccggac ccgtttcgac gcccaggtgg cgcaatcgca ggaggccgta cgcaccctcg
     3721 aaggcgagtt ggccgcggcg atccagcagc gggaagggtt ccagcgggat tggcagaagg
     3781 cggcggacga cgcccgccgc tggaatcagc aattgatgga acaggagtcg cagcggaagc
     3841 tcctcgagga ccgtttggcg cagctccggc aggaactcat tcagcgggga gaagaggcgc
     3901 ggtcccgctt cgagccgcaa ctgacgcagg cgcaggaggt catccggacg cttcaggaca
     3961 acgtctcgaa gctgatgcag gagaaggagt cctatgagcg tcaggcccgc aaggcggaag
     4021 aggaagtggc gcgttcttcc cagcgcgttc aggaactgga ggcggaaaaa cgcctgacgg
     4081 acgaacgctt gaatcgtctg agggaagaga cggcgacgct ggaaattacc cttcgagaca
     4141 agatagaatc ggaatatcgg gggctggcgg agcgtgccga aatggagcag cgccaggtgc
     4201 accagtgggc gcaggagacc gttcggtcgg cggagcagaa gttgcgcgag caggcctctc
     4261 gcgggcagca gaagatccag gcgctgcagt cttccatggc cgaagacctt gccgcgttcg
     4321 acaaggaact cgccgcgctg cgcgcggaga ggacccagct ggtgcacgag cgggactccg
     4381 tcgtcgagtc gctccgtaaa tcggacgaga agttccagga gtcctatcag cgatgggacc
     4441 aggaaagggc gttgttgagg gaatccctga aagaggcgga gaaatccctg caggaactcc
     4501 ggtcccgggc ggaatccgat cgccgggcgt gggaagagga cttccagcgc cgcgagaaga
     4561 gttcgacgga aaccatatcc acccaatggg aggagcgcgt gaagatgctt caagcgcacg
     4621 tggaggatga gcgccggctc tggacggaaa aattgaagac gctcgacgag aacgtggccc
     4681 ggctggaggg ggagaaacgt cgtttcctcg accagcgcaa cgaggagatc tccgcgctga
     4741 ggaaacagta tgagaacgag ctggagaatc tgcggcaggc gctgatgcgc cgtccgcggg
     4801 ggcttttcgg gagtgtcggc gcctggctca accagccggt gatcgaggtc aacctgggcg
     4861 cgcgcgataa aaggagagag tgaccatgtc gttgtcgatg gagcatatcc gtcaaaagtg
     4921 gctgaaacgc ctcggtgagg cggcgcagga tttcgggtcg gcggaggagg acctttccga
     4981 agtcctgatg ccggacatgg agccgccccc caaacccacc ccgatccttt tccggcgttc
     5041 ccacgggggg gtcttgccga gggaaacggg ccatttggag gaggggttcc atgcgttgag
     5101 ggaatccctg gcccagacca aactgcagcg gcagcaggcg gagacggaac tggagaaaat
     5161 ccgcaaggac tacgtggcgg cggacggacg catcaacgag ttggcgatga acttggcggc
     5221 gttggaggag aagaaggctc agctggagaa gagtttggcg tctttgcagg acgccctccg
     5281 gacgcgcgac gaggagaaca ccgttctgcg cggggagacc gcgcacctgc aggcgaccat
     5341 cgccggcaag gacaagtccc ttgaagagat gagggccctg cagaaagagc tggaggcgca
     5401 gctggaagag ttgcgcgccc acatggcggg gtccgccaag gagagcgagc gggcctatgc
     5461 cgaggcggaa cgccgggtgg ccgaaagcgc ggcggtgatc cgggaaaaga ccgcccgtat
     5521 cgaggaactg gaacgggaac tttcgacgga ttccgaggag tcccggcagg attttcagga
     5581 cgctttggag aaaaaggaga cggaaatccg tctcctgtcc gaaaacatca agcggctcag
     5641 tcagcagaac aaggacatgc tggccgccct tcaaaaattc cagacgtccg gcggtctggc
     5701 ggacccgtcg gggatggcgc cggcggcgga accggacgcg gcggcccggg agaagatcga
     5761 gtcgctggaa ttggcgttgg cctcccgcga caggatgatg accgaccagg agaagttgat
     5821 cgcggacaag cagaagacca tcgaggaact gttccggaag gtgcgcgaac tcgacgcctg
     5881 cgcggccggc cgtcccgcgg actccagcga gacggcgaat ttgaccaagc aggtcgaact
     5941 cctcctggag gagaataaga agtggcggaa agaggcggag gagaaggcgg ggttttcccg
     6001 ggaaacgtcc atgcgggccg agtctttggc ggcggagaac gcggcccttt cggaccggct
     6061 gaaggcggcg gagtcctcgt cggcggatcg cgcgtcgttg gagtccaagc tggcggacca
     6121 ggaaaaactt ttcgaacgga agttcgagga ctggcaagtc cgcatcagcg aaaaagagcg
     6181 gcgcctcgga gaggtggaga aggcgaaagt ggagcttgag gcggaactga aggcgcgccg
     6241 cgacgagttt gagaaaaatg tccgtctgtg cgacgagctc cggatgaaga acgaagaatt
     6301 ggaaacgcag ttgcgcctgc tggtgatgaa cagcgaggcg atcgaccgga tggagaagaa
     6361 cctgcgggag acgttccgcc tcatgacccc gggcggcacg acgttcccca tcccgcgccc
     6421 ccgggacgag caacccgcgg agcaacgtcc cggtttcttc gggcggctgc tgaattggtg
     6481 gcgcgccccc ctggtgaaaa tccccaaaaa atcttcgacc gcggcccccg cgccggcggt
     6541 gcagcgtatc cagcagaacc tgaaagcccg gagaaacgtt aaacctcaat gagccgggaa
     6601 ccttccccgt ccctcctgaa aaggatatgg gatcggatac tgtcctggct gggactggcg
     6661 gacgacgccc aggcgggcgc ggcggccgcc gaacaggaac gcctgaccct catcgtccag
     6721 cagcacgagg cggagcttct ccggctgcgg cgtctcctgt cggataccca gagcagtctt
     6781 cagcggtccg agcaggaacg aaacaaatcc ctcgacaccc tgaacgaccg ggagatccgg
     6841 ttggagaagg agaagtcccg cgccgccgtg gccggagtcc tcatcaagga ttacgacatt
     6901 gagttgaaaa agatacgccg ggaaaaattg acccaacagc gtatttttga agacaagatc
     6961 cacgccctcc tcgaaaaact gaacgaactc cagcggagtc tgcccaccgc gccgtccgcc
     7021 aacctgccgt ccccggtcgt cgccaagtct ctggccctgg aaaaacaggc gctgcagcag
     7081 gaactccacg aaacccagga gaagctggcg ctcctggagg ataaactcga ccgcgagacc
     7141 tccgcgcgac tggacgtgca aaaacaatcg gcgagcctgc agtccggttc cgcggaactc
     7201 caggaaaaat tccgtgccgc cgaatccgcc ctgtggaacg agaagaccaa gacggaggcc
     7261 ctggagaagg agaagaccgt tctgcagaaa caggtgatgg aactgcagaa gacgctggag
     7321 gcggtgaaga acgtggacag cgtgcaggag ccggcctcgg tctctcagga agtgatcgct
     7381 ctgcgggaga gggttcaggc gctcgacgag gccctgcggt cggcgcgggc cgcggcggcg
     7441 gggggcggtg gagggccggc gcccgtttcg gacgaggaac tttcgccgat cccccacggg
     7501 gttcagatgg agttggatac cctgcggtcg gctttggaac aggagcgggc tcgatgggag
     7561 gaggaacgcc tgaggaccga ggcggcgttg gaggaagaga agcggcgggc ggacaaggaa
     7621 acccgtctgc gccgcacgct ggaggagcgc accacggccc tttcccggga aaccgagaag
     7681 tggcgttcga cgcacgaaga ggcgcggaaa cagtgggaag acgagagaaa gcggcttttg
     7741 gaacagtggg ataaggagaa agaacaccgc ttggaggagt tggaaatgca ccgtcaggcg
     7801 gaggaccggg cggggtctct gttggaggag atcgagacgc ttcggaaaac gctggagaaa
     7861 gagagagcct cctgggcgaa cgagcggacc gacctccagc aacggcagga acgggaccgg
     7921 cgcgaatggg gggaacggct cgatcgtgcg gccaagctgc gcggcgatcc gggagagctg
     7981 gaggagatac gcaaggggtt cgagcaatcc cgagccgtct gggagaaaga gcggggggat
     8041 ttgacggccc gctgggaaaa ggaacgggag gagtggcgcg aaaggctgga ggcggcggcc
     8101 ggttcctcgt cggcggccgg cgggttcacg gccacgcagg aggaggtcga ccgtctgttg
     8161 ggagaactgg atgcgttgac cgccgagaac gcttttttga aggaccagtt ggggtgggag
     8221 gagtcgaaac cgtcatgaaa gccggggtgc cgcgtgtccg ttaaaggggg tctcgcgtga
     8281 ccaatccgcc ccgtccgcag ttgaacattt cggcaaaact agacacccgc ctgcgcggca
     8341 cgctggtggc gcgcgttcag acggcccaac tgctcaagat gggcgagccg gacatcgcca
     8401 aactcatcaa ggagatcgag tccgacccgc ttttcttgaa aatgctttat ccgcccaaac
     8461 ccgactggaa gatcgtccga tttcaacccc atccccgcac cagcctgtcg cacgctttct
     8521 acgaactcaa cgatcagctc ctgccgcagg acacgcccgc cgacgtggtt caactcctgg
     8581 aaaacaggcg gggtctccgc gagaagattc ggggaatggg accggaggcg tttgagcgct
     8641 atttcctccg cgccgacgaa aatctgtcgc cggaggaggc gggggaggcc tgcgggttga
     8701 ccccggagga ggttcggcag gtccaggatt tcctcctgga attctccgtg cacgccgagt
     8761 ttttcgaccg ctccagcaaa tcggttccgg ggatccgcgt cgtccgtttg gggcgggtcc
     8821 agatgacgga cgacgacgtt ttcatcgaat ggaactcgcc ccacctggcg cgcgggcgct
     8881 acgagattca atacgaccgg atggaggagc tgatccggaa gggccttctg gcgccggagg
     8941 aaaaacggca cctgaaagcc ttgatccacc gtctggaact cgtcaattgg cggcaaagcg
     9001 cgctctaccg catcgtctac ctgctgtgtt atacccagcg cgattatctg gcccagcggg
     9061 acaccatgaa gcggcggtcc atcacccagc ggcggatggc gcgtcagttg agcgtggcgc
     9121 cttccaccgt caaccgcgcc gtgcaggggc ggagcctaat cctgccgtgg ggggaggagc
     9181 ttctcctgga ggagctgttc tgttcgcgca aggagctgtg tttggacgcg ctcgacgccc
     9241 tggacgccgc ggacaaggat ttcaaggaca ggacggacaa cgaactgcgc gagcggctga
     9301 aaaaaagtct ggggttcacc gtcccgcgcc ggaccgtcaa tacctaccgg cgccagctcc
     9361 agtcccggtc gccgagctcc tcggggtccg gcgaggtccc ttgatttaat ggtcgcttat
     9421 tgttacttta atgaaccggc gcgagttgtt ctggagcgaa atcgccgccg ggcctaaaat
     9481 gatcaaggtc caccgtctca acggcacgga aataaccgtc aacgcggaat tgattgaaac
     9541 cgtg
//
LOCUS       DAOTFD010000077         9526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5081_length_9526_cov_4.336290, whole genome shotgun sequence.
ACCESSION   DAOTFD010000077 DAOTFD010000000
VERSION     DAOTFD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5081_length_9526_cov_4.336290"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="P5079_06055"
     CDS             <1..935
                     /locus_tag="P5079_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HRY29586.1"
                     /translation="RNESEGDVKLGTGGRDVKKDVGGISFLQGLNALVEDKTTKVVLL
                     VSKPPYPDVLKKILAEIKKVKKPVVSIFLGGDTAGLRKAGVHAFDTLEEAALAAVSLS
                     GGGKTDKIAAQLAARDKKLESLAKAEAKRLAKGQKFCRGLFSGGTFCSEALLILSEML
                     GDVHSNIPLKPGLKLQNSMKSVANTVIDMGEDEFTVGRPHPMIDYSLRNKKILEEANA
                     SETAVILLDVVLGYGSTPQPLEDILPALQEAFAVVRKNRRGLSVVCSVTGTDKDPQNR
                     SKVVQGLEKAGCLVQPSNAAACRLAGRIVKSANV"
     gene            948..2348
                     /locus_tag="P5079_06060"
     CDS             948..2348
                     /locus_tag="P5079_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRY29587.1"
                     /translation="MAMKNLFDKELKVLNIGLASFKATLDENKTSAVHVDWKPPVDVP
                     KEVMAVIHKNRKTIGQANEKAAKIILAGKPALIGLEKAGDVVPGMKKNLILHAGPPIT
                     WDRMCGPMRGAVMGALMYEGLAKTADEAKKLASSGKIEYSPCHEHQSVGPMAGIISFS
                     MPVFVVKNEEYGNLAFATMNEGLGKVLRYGAYGDDVLQRLKWMETALYPVLKKALARL
                     TRIDLKNLIAQALHMGDELHNRNRAATSLLLRLLAPAVLRTMEDPETSAKVLEFVNGN
                     DHFALNLSMAASKATLDPARNIPHSSVAVVMARNGTDFGVQLAGTGNAWFTGPAPVPD
                     ALFFPGYTKADANPDIGDSAITETNGLGGFAIAAAPAIVQFVGGSAADAANYTLQMYE
                     ITAAENNAYQIPGLNFRGTPTGIDVVRVVEKGIFPFIDTGVAHKEPGIGQVGAGVLSA
                     PAEPFKKAVEGLAKTL"
     gene            2423..3079
                     /locus_tag="P5079_06065"
     CDS             2423..3079
                     /locus_tag="P5079_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRY29588.1"
                     /translation="MKKILTPEEAVENIHDGAIIMAGGFMCCGQALRLADALLAKGSK
                     DLTLITNDAGFPDGGMGKLIVAGRVKHLIASHIGLNPAAGQKMNSGEMRVDLVPQGTL
                     VERIRCGGAGLGGFLTPTGVGTAVEEGKKKIKVDGRDYLLETPLKADFGFIKANVCDT
                     MGNGFLAKSTKNFNIVMAMASQHTILETDRLVKRGELDPEQVNLPGVFINAIAHEGKK
                     "
     gene            3076..3738
                     /locus_tag="P5079_06070"
     CDS             3076..3738
                     /locus_tag="P5079_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRY29589.1"
                     /translation="MSAAKDLKRERIARRIVRELPDNALVNLGIGMPTLVANYLPKDK
                     TIFLHSENGFIGLGPHPEKGKEDKDIVNAGGAPVTIMPGGCFFDSALSFSIIRGGHVD
                     VTVLGALEVDEEGNLANYMIPGKMVPGIGGAMDLVTGAKKVIIAMEHVNKYGAPKILK
                     KCTLPLTAKKEVDIIVTDMAFIRVTQNGLVLEEIAEDTTVEEVVQNTGAKLVVHPKIL
                     EF"
     gene            3779..4591
                     /locus_tag="P5079_06075"
     CDS             3779..4591
                     /locus_tag="P5079_06075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY29590.1"
                     /translation="MNNNEFIDLMTKSKMYDLTQPLSVHTPPWPSYIPLSVQYFKRIA
                     GAHMGQGANGQVITTSNHVGTHMDGEIHFHASGRSIGQVPMSEWIGPGAVVDISKEMG
                     DYDLYDPDLLMKKVEIKKNDILLINTGYHRYAWYEKDSDEVRYFVKHPGPGPKFHEWC
                     LDMKFKWIGVDCGSADHPMNTIIRNWHPKLFIEAEKKLKKNYKKSWDEMFPPEIYYQV
                     MHLKLFPKGIVHAENIGGEIEKIGNKRVYVGFFPLKGMELESAMGRCVAWLP"
     gene            4636..5526
                     /locus_tag="P5079_06080"
     CDS             4636..5526
                     /locus_tag="P5079_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRY29591.1"
                     /translation="MPIAHEFDYHKPKTLPEAVKLLAEYRGKAKPLAGGTDLIVWLKG
                     GLEKPEAVVDIKALRELGRIVWKKDEALIGALITFSELIDNADVRKKLPVLWEASRTV
                     ASVGVRNRATLAGNICSAVPSLDSAPALLVYDAVVHLQGPRGKRQMPLKDFFMGPKKT
                     ALAPGELVTGVAVRIPKKKSGGCYAKLGRYAGEDLAQVGLAVLALADNTYRVAHCAVG
                     PVARRAEKIEGLLNGKRLSDILLEEAVRLVDKEISPISDIRSSKEYRLHMTKVMLRRA
                     LWAAVNRLAGSGPVAGSSLI"
     gene            5537..6010
                     /locus_tag="P5079_06085"
     CDS             5537..6010
                     /locus_tag="P5079_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY29592.1"
                     /translation="MKNISIQLKLNGVRRTLEVEPSDVLLDVLRDKLGVKSPKVGCDR
                     GDCGSCTVFMNGKTVRSCLVLAVEADGADVVTLEGISKNGPTRLQKEFVKRNSFQCGF
                     CAPGVILSAEEALKKHPRASDEEIKEHIAGNLCRCTGYKPILDAIKTVASGKASK"
     gene            6025..8421
                     /locus_tag="P5079_06090"
     CDS             6025..8421
                     /locus_tag="P5079_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY29593.1"
                     /translation="MQKLSQKLEELTVPPISVGPAGGHRVVGKSVERIDGWEKVSGAA
                     KFLEDLDFGPNLLYAALVESPVAHAKIKKIDVRDALKVPGVVKVVTGKDFPYKFGLYM
                     KDRYVFAYDRVRFVGEQVAAVIAWSPKAALRGAAKVKVDYEELPIVLDPVRAIEKNAT
                     LIHPDLGNYPHVPWFFPKADTNIAHWRKTRKGDWEKGFREADAVLEDTYFVPRYAHCA
                     IEVHAAVGLYDMSGRLTVWTASQSPFTQRNVFAEALAPLGISHKDVRVITPYVGGGFG
                     GKAGVSMEIIGAALAMTVKGHPVKVLWNRAQEFCNTYQRQGVVAKIKMGVKKNGKITA
                     LEHSLYWDAGAYVEYGANVVNAAGLSATNPYRVDNVKIDSICIYTNLPPGGPYRGFGY
                     SEFSFGIESHVTRMAQKIGLDPVEFRRRNHIKEGDTLPYGAHMNPNGLRESIDKVAAE
                     IMWGSKAKSDDPKKAVGKSLVLFWKSPAMPPNAASSAFLKFNEDGSINILISGMEIGQ
                     GLLTAMAQIASEILSVPVAKIRVETPDTDRNPYEWQTVGSHVTWGCGNAVKKAALDAR
                     DQIFELVQRALHLEKDTLYLEDERVKCRTKPDFELSFASFVIAGIQTEDHTFKGGPIM
                     GRGVFMPEFASAIGDPETGQGGHPNVHYTTGAAGLILEVDKETGKMKIRKVALAVDVG
                     KAINPDLIRGQVVGGLLQGFATVLYEDMRYSEKGKLLNPNFSDYKIPTSLDVPEEIVP
                     IIVEVPQPDGPFGARGVGEHTMIPTASLVANAVEDALGVRIKSMPITAEKVALTVSKG
                     "
     gene            8469..>9526
                     /locus_tag="P5079_06095"
     CDS             8469..>9526
                     /locus_tag="P5079_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRY29594.1"
                     /translation="MSGTQQGHGNTPTPGHAGPEKIKGKDFAGADMSQRDLSWVNLSH
                     GSLKGVNFSRSILVHAHLDYADIAETDFSESNLTHADFKNTRLRRVTAKKAEMAAVQF
                     NGGRVEGGDFSGAHLQGSNWEFVECKGTDFSRSNMAGANLLRGTFDQCDFSEARLVGA
                     DLSESRLKNTKMAGADLTAANLEGAELKGVDFRDARVSGARFWSAHGLSKEQKTFIVE
                     NGGSLYSPRMLKLIQCARFFKTNVYAQIGVLAFLLGLTVFGYRYSENPHHRNNRWVMD
                     KAGKYLNRGDHDKALALYRLLLQRPGQENPLQIYSSMIQTYFQMNRFDKILEVYAEAV
                     RSTDAPGALADIELELAN"
BASE COUNT         2094 a         2790 c         2958 g         1684 t
ORIGIN      
        1 cgcggaacga gtccgagggt gacgtaaaac tggggaccgg cggccgggac gtcaagaaag
       61 acgtgggcgg gatctcgttc ctgcaggggt tgaacgcttt ggttgaggat aagaccacga
      121 aggtggtttt gctggtgtcc aaaccgccct atccggacgt tctgaaaaaa attctggccg
      181 aaatcaagaa agtgaaaaag ccggtggtgt ccatcttcct cggcggcgat accgccggac
      241 tccgcaaggc cggggtccat gctttcgaca cgctcgaaga ggcggcgctg gcggcggtgt
      301 ccctctccgg cggagggaaa accgataaaa tcgcggcgca gttggcggcg cgcgataaaa
      361 agctggagtc cttggccaag gccgaagcga agaggctggc gaaagggcaa aaattttgcc
      421 gcgggttgtt cagcggcggc acgttctgca gcgaggcgtt gctgatcctc tcggagatgc
      481 tcggcgacgt tcattccaac atcccgctga aaccgggact gaaactgcag aactccatga
      541 agagcgtggc caacaccgtc atcgacatgg gggaggatga gttcaccgtc ggccgcccgc
      601 atccgatgat cgattattcg ctgcgcaata aaaaaatcct ggaggaggcg aacgcctccg
      661 agaccgcggt catcctcttg gacgtggtgc tgggatacgg ttcgaccccg cagcctctgg
      721 aggatatcct gcccgccctc caagaggcgt tcgctgtcgt tcggaaaaac aggcgaggcc
      781 tgtcggtcgt gtgttccgtg acgggaacgg acaaggaccc gcagaaccgg tcgaaggtcg
      841 ttcagggcct ggaaaaggcc ggctgcctcg ttcagccgtc caacgccgcc gcctgccgcc
      901 tggcggggcg gatcgttaaa tccgcgaatg tctagggggc gatgaccatg gccatgaaaa
      961 atttgttcga caaggaactg aaagtcctca acatcggatt ggcgtccttc aaggcgacgc
     1021 tggatgagaa caagacctcc gccgtgcacg tggactggaa accgcccgtg gacgtcccca
     1081 aagaggtgat ggcggtcatc cataagaacc ggaaaaccat cgggcaggcc aacgagaaag
     1141 cggcgaagat cattttggcc gggaaaccgg cgctcatcgg attggaaaaa gccggggacg
     1201 tcgtccccgg catgaagaag aatctgatcc tgcacgccgg tccgcccatc acctgggacc
     1261 gcatgtgcgg tcccatgcgc ggcgcggtga tgggggcgct gatgtatgag gggctcgcca
     1321 agaccgccga cgaggcgaaa aaattggcgt cgtccggcaa gatcgaatat tcgccgtgcc
     1381 atgagcacca gtccgtcggg cccatggcgg gaatcatctc cttctccatg cccgtgttcg
     1441 tcgtcaagaa cgaggaatac ggcaacctgg ccttcgccac catgaacgag gggctcggca
     1501 aggtgctgcg ttacggcgcc tacggcgacg atgtgcttca gcggctcaaa tggatggaaa
     1561 cggctctcta tcccgtcctg aaaaaagccc tggctcggtt gacgcggatc gacctgaaga
     1621 acctcatcgc ccaggccctt cacatggggg acgaacttca caaccgcaac cgcgcggcga
     1681 cctcgttgct cctgcgcctc ttggccccgg cggtgcttcg caccatggag gatccggaga
     1741 cctccgccaa agtgctggag ttcgtcaacg gcaacgacca ttttgcgttg aacctctcca
     1801 tggcggccag caaggccacc ctggatcctg cccgcaacat cccgcacagc agcgtggcgg
     1861 tggtgatggc ccgcaacggt acggatttcg gcgtgcagct ggcggggacc ggcaacgcct
     1921 ggttcacggg ccccgcgccc gtgccggacg cgctgttctt ccccggctac accaaggcgg
     1981 acgccaaccc cgacatcggc gacagcgcca tcaccgagac caacgggctc ggcgggttcg
     2041 ccatcgccgc cgccccggcc atcgtgcagt ttgtgggagg cagcgccgcc gacgccgcca
     2101 attacaccct gcagatgtac gagatcaccg ccgccgagaa caacgcctac cagatccccg
     2161 gactgaattt ccgcggcacc cccaccggta tcgacgtggt gcgggtggtg gaaaaaggga
     2221 ttttcccgtt catcgatacc ggcgtggcgc acaaggaacc gggcatcggc caggtggggg
     2281 cgggcgtgct gagcgcgccg gcggaaccgt tcaagaaggc cgtggaggga ttggcaaaaa
     2341 ccttgtgagc gggtcgcgat ccggggaaaa cggagccccg gcgttttttg ctaagatttg
     2401 acgctctgga gataggaacc tcatgaaaaa aatactgacc cccgaagaag ccgtcgaaaa
     2461 catccacgac ggcgccatca tcatggccgg cggtttcatg tgctgcggcc aggccctgcg
     2521 tctggcggac gccctgctgg ccaaggggtc gaaagacctc accctcatca ccaacgacgc
     2581 ggggttcccc gacggcggca tgggcaagct catcgtggcc ggccgcgtga aacacctcat
     2641 cgcttctcac atcgggctca atcccgccgc cggccagaag atgaattcgg gtgaaatgcg
     2701 cgtggacctc gtgccgcagg ggaccttggt ggaacgcatc cgttgcggag gggcggggct
     2761 gggcgggttt ctgaccccca ccggcgtcgg caccgccgtg gaagaaggca aaaaaaagat
     2821 caaggtggac gggcgcgatt acctcctgga aacgccgctc aaggcggatt tcgggttcat
     2881 caaggccaac gtctgcgaca ccatgggcaa cggatttttg gccaaatcca cgaagaattt
     2941 caacatcgtg atggccatgg cgtcccagca cacgatcttg gagaccgacc gtctggtgaa
     3001 acgaggcgaa ttggatcccg aacaggtcaa cctgcccggc gtcttcatca acgccatcgc
     3061 tcacgagggg aagaaatgag cgccgccaaa gatttgaaac gcgaacgcat cgcccgccgg
     3121 atcgtccggg agctgcccga taatgccctc gtgaatttgg gcatcggcat gcccaccctg
     3181 gtggcgaact acctccccaa agacaagacg atcttcctgc actcggagaa cgggttcatc
     3241 gggttggggc ctcacccgga aaagggcaag gaggacaagg acatcgtcaa cgccggcggc
     3301 gcgccggtga ccatcatgcc cggcggctgt ttcttcgatt ccgcgctctc cttctccatc
     3361 atccgcggcg gccacgtgga cgtcaccgtt ctgggggcct tggaggtgga cgaggagggg
     3421 aacctcgcca actacatgat tcccggcaag atggtgcccg gcatcggcgg cgccatggat
     3481 ctggtcaccg gcgccaagaa ggtcatcatc gccatggagc acgtcaacaa atacggcgct
     3541 ccgaagatat tgaagaagtg caccttgccg ctcaccgcca aaaaagaggt ggacatcatc
     3601 gtcaccgaca tggccttcat ccgcgtgacc cagaatggac tcgtcctgga ggagatcgcc
     3661 gaggacacca ccgtggaaga agtggtgcag aacacgggag cgaaactggt cgtccacccg
     3721 aaaattttgg agttttaggc cccgtttggg gttctttcga cataaaaagg agcttcccat
     3781 gaacaacaat gaattcatcg acctgatgac caaatcgaag atgtacgacc tcactcagcc
     3841 gctgagcgtg cacaccccgc cctggccgag ctatatcccg ctttccgtcc agtattttaa
     3901 gcgcatcgcc ggcgcccaca tgggacaggg ggccaacgga caggtcatca ccaccagcaa
     3961 ccacgtgggc acgcacatgg acggcgagat ccatttccat gcctccggcc ggtccatcgg
     4021 gcaggtgccc atgtcggaat ggatcggtcc cggcgcggtg gtggacatct caaaggaaat
     4081 gggggattac gacctctacg acccggattt gctgatgaaa aaagtcgaga tcaaaaagaa
     4141 cgacatcctc ctcatcaaca ccggatacca tcgctacgcg tggtatgaga aggactccga
     4201 cgaggtccgc tacttcgtga aacatccggg ccccggcccc aagttccatg aatggtgtct
     4261 ggacatgaaa ttcaagtgga tcggcgtgga ctgcggttcg gccgaccacc ccatgaacac
     4321 catcatccgt aactggcatc ccaagctctt catcgaggcg gagaaaaagc tcaagaagaa
     4381 ctacaagaag tcctgggatg agatgtttcc gccggagatc tattatcagg tgatgcattt
     4441 gaagcttttc cccaaaggca tcgtgcatgc ggagaacatc ggcggcgaga tcgagaagat
     4501 cggcaacaaa cgcgtgtacg tgggattttt cccgctgaag gggatggaac tggaatccgc
     4561 catgggccgc tgcgtggcct ggctgccctg acggcccctg tccgttttgc gcaaaaaccg
     4621 taagaggaga cgctcatgcc catagcgcac gagttcgatt atcacaagcc caaaacattg
     4681 cccgaagcgg tgaaactgct cgccgaatac aggggcaagg cgaaaccttt ggcgggcgga
     4741 acggatttga tcgtctggct caaagggggc ctggaaaaac ccgaggccgt cgtggacatc
     4801 aaggcgctgc gggaactggg ccggatcgtc tggaaaaagg acgaagccct catcggggcg
     4861 ctcatcacct tcagcgagct catcgataac gccgacgtcc gaaaaaaact cccggtgctg
     4921 tgggaggcga gccgcacggt ggcgtcggtc ggcgtgcgta accgcgccac cttggcgggc
     4981 aacatatgtt cggcggtgcc ctcgctcgac agcgccccgg ctctgctggt ctacgacgcc
     5041 gtcgtccatc tccagggccc cagaggaaaa cggcagatgc cgttgaaaga ttttttcatg
     5101 ggtcccaaga agaccgctct tgccccgggc gagcttgtca ccggggtggc cgtacgcatt
     5161 ccaaagaaga agagcggcgg ctgctacgcg aagctcggcc gttacgccgg ggaagacctg
     5221 gcgcaggtgg ggttggccgt cctcgcgctg gcggacaaca cctaccgcgt ggcccactgc
     5281 gccgtggggc cggtggcacg ccgggcggaa aaaatcgagg ggctcttaaa cgggaagcgc
     5341 ttgagcgaca ttctcctgga agaggcggtt cgtttggtgg acaaggagat cagccccatc
     5401 tccgacatcc gttccagcaa ggaataccgt ctccacatga cgaaagtgat gttgcggcgg
     5461 gcgctttggg ccgccgtgaa ccgcctggcc gggagcgggc cggtggccgg ctcgagcctg
     5521 atttaagggg gatcccatga aaaacatttc aatccaattg aaactgaacg gcgtccggcg
     5581 gacgctcgag gtggagccca gcgacgtgct gttggacgtt ctgagggaca agctcggcgt
     5641 gaagagcccc aaggtggggt gcgaccgggg agactgcggc tcctgcaccg ttttcatgaa
     5701 cggaaagacc gtgcgcagct gtctggttct ggcggtggag gccgacgggg cggacgtcgt
     5761 cacgttggag gggatctcca aaaacggtcc cacgcggctg cagaaggagt tcgtcaagcg
     5821 caattcgttc cagtgcggtt tctgcgcgcc cggcgtgatt ctttccgccg aagaggcgct
     5881 gaagaaacat ccgcgcgcct cggacgagga aatcaaagaa cacatcgccg gcaacctgtg
     5941 ccgttgcacg ggctacaaac ccatcctcga cgccatcaag accgtggcgt cgggcaaggc
     6001 gtccaagtag gtccgggggc catcatgcaa aaactttcac agaagctgga agaactgacc
     6061 gtgccgccga tttccgtggg cccggccggc ggccaccgag tggtcgggaa gtccgtcgag
     6121 cgcatcgacg gctgggagaa agtttccggc gccgcgaagt tcctggaaga cctggatttc
     6181 gggcccaacc ttctctacgc cgccctggtg gagagccccg tggcgcacgc caagatcaag
     6241 aagatcgacg tcagggatgc cttgaaggtg cccggggtgg tgaaggtcgt cacggggaaa
     6301 gatttcccct ataagttcgg cctctacatg aaggaccgct atgtcttcgc gtatgaccgc
     6361 gtacgcttcg tcggggagca ggtggccgcc gtcatcgcct ggagtcccaa ggccgccctg
     6421 cgcggcgccg ccaaggtgaa agtggactac gaggaactcc cgatcgtcct cgatccggtg
     6481 agagccatcg aaaaaaacgc caccctcatc catcccgatc tgggcaacta cccgcacgtg
     6541 ccgtggtttt ttcccaaggc cgacaccaac atcgcgcatt ggcggaagac ccggaagggg
     6601 gattgggaaa aagggtttcg ggaggcggac gccgttctgg aagacactta ttttgttccg
     6661 cggtatgccc actgcgccat cgaggtgcac gcggcggtgg ggctttacga catgtccggg
     6721 cggctcaccg tctggacggc gtcccagtcg ccgttcacgc aacgcaacgt cttcgccgag
     6781 gcgctcgccc ctctcggcat ctcgcacaag gacgtgcgcg tgatcacgcc gtacgttggc
     6841 ggcgggttcg gcggcaaggc cggcgtgtcc atggaaatca tcggcgcggc cctcgccatg
     6901 acggtgaaag gccacccggt gaaggtgctc tggaaccgcg cccaggagtt ctgcaacacc
     6961 tatcaacgtc agggcgtggt ggccaagatc aagatgggcg tgaagaagaa cggcaagatc
     7021 accgccctgg aacattccct ctattgggat gccggcgcct atgtggaata cggcgccaac
     7081 gtggtgaacg cggccggcct ttcggccacc aacccctacc gggtggacaa cgtgaagatc
     7141 gattccatct gcatctacac caaccttccg ccgggcgggc cctatcgcgg attcggctat
     7201 tccgaattca gtttcggcat cgagtcacac gtcacgcgga tggcgcagaa aatcgggttg
     7261 gaccccgtgg agttccgtcg acgaaaccat atcaaggagg gggataccct gccctacggc
     7321 gcgcacatga accccaacgg ccttcgcgaa tccatcgaca aggtggccgc ggagatcatg
     7381 tggggttcca aagcaaagag cgatgatccg aaaaaagcgg tgggcaagag cctggtcctt
     7441 ttctggaagt cgcccgccat gccgcccaac gccgcctcct ccgcgttctt gaaattcaac
     7501 gaagacggaa gcatcaacat cctcatctcc ggcatggaga tcggccaggg actgctgacc
     7561 gccatggcgc agatcgcctc cgagatattg tcggtgccgg tggccaagat ccgggtggaa
     7621 acgcccgaca cggaccgaaa cccgtacgaa tggcaaacgg tcggttcgca tgtcacctgg
     7681 ggatgcggca acgccgttaa aaaggccgct ttggacgccc gggatcagat attcgaattg
     7741 gtgcagcgcg cacttcatct ggagaaagac accctgtatc tggaagacga gagggtgaaa
     7801 tgccgcacca agcccgactt tgaactgtcg ttcgccagtt tcgtcatcgc cgggattcag
     7861 acggaagacc acaccttcaa aggcggtccc atcatgggtc gcggtgtctt catgcccgaa
     7921 ttcgcctcag ccatcggcga tccggagaca ggccagggcg gacaccccaa cgtgcactac
     7981 accacggggg cggcgggact gattctggag gtggacaagg agacgggcaa gatgaagatc
     8041 cgcaaggtgg ccctcgccgt ggatgtcggc aaggccatca acccggatct gatccgcggc
     8101 caggtggtgg gaggtctgtt gcaggggttc gccaccgtgc tctatgaaga catgcggtac
     8161 agcgaaaagg ggaaactttt gaaccccaac ttctccgatt acaagatacc cacctccctg
     8221 gatgtgccgg aagaaatcgt gcccatcatc gttgaggtcc ctcagccgga cggtcctttc
     8281 ggggcgcgcg gggtcgggga acacaccatg atccccacgg cttcgctggt cgccaacgcc
     8341 gtcgaggacg ccctgggggt gcgcatcaaa tccatgccca tcaccgccga aaaagtggcg
     8401 ctgaccgtca gcaaggggta agggcgcgtc gtccggggcg gtcaaactca catgagaaag
     8461 gaatttcgat gagcggaacc cagcagggtc atggaaacac tcccacgccc ggccacgcgg
     8521 gcccggagaa gatcaaaggc aaggattttg ccggcgcgga catgtcccag agggatcttt
     8581 cttgggtcaa tctgtcccac ggttccctga aaggggtcaa tttttcacgc agcattttgg
     8641 ttcacgccca cctggactat gccgacatcg cggagacgga tttttccgag agtaacttga
     8701 cccatgccga ttttaagaac acccgtttga ggagggtaac agcgaaaaaa gcggagatgg
     8761 cggcggtcca gttcaacggc ggccgcgtcg agggggggga tttttccggc gcgcatctgc
     8821 aggggtcgaa ttgggaattc gtggagtgca agggaacgga tttcagccgc tccaatatgg
     8881 ccggcgcgaa tttattgcgc ggcacgttcg accagtgcga tttcagcgag gcccgccttg
     8941 tcggcgccga tctgtccgaa tcccgattga aaaacacaaa aatggccggg gccgacctca
     9001 cggcggccaa tctggagggg gccgaactta aaggggtgga tttccgagat gcgcgggttt
     9061 ccggcgcccg cttttggtct gcacacggac tttcgaaaga gcaaaagacg ttcattgtgg
     9121 aaaacggcgg gagtctttat tctccccgga tgttgaaact gatccaatgc gcccgatttt
     9181 ttaagaccaa cgtgtatgcg caaataggag tccttgcctt tctcctcggc ctgacggttt
     9241 tcggatatag atattcggag aacccacatc atcgaaacaa caggtgggtg atggacaaag
     9301 cgggaaaata tttgaatagg ggcgaccacg acaaagccct cgccttgtac cgtcttttgc
     9361 tgcagcgtcc cggccaggaa aatcccctgc agatatattc atccatgatt cagacgtatt
     9421 ttcaaatgaa ccggttcgac aaaatactgg aagtttatgc ggaagcggtc cgaagcacgg
     9481 atgccccggg ggcgctggcc gacatagaac ttgaactggc caattt
//
LOCUS       DAOTFD010000078         9505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5101_length_9505_cov_3.937354, whole genome shotgun sequence.
ACCESSION   DAOTFD010000078 DAOTFD010000000
VERSION     DAOTFD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5101_length_9505_cov_3.937354"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="P5079_06100"
     CDS             <1..273
                     /locus_tag="P5079_06100"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HRY29595.1"
                     /translation="LLLRRFRRNRRGFEVRRYVVTGGETAFRFVRLLGRGFWRVADRV
                     ERGLPLCVEDIQRSPRRRPCWMVLKPGGFGSETALIKSVRRLQRVL"
     gene            263..817
                     /locus_tag="P5079_06105"
     CDS             263..817
                     /locus_tag="P5079_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRY29596.1"
                     /translation="MFSDFFTYGRVLWEAGHLHVTSGNMSVKKGSVIYVTRTGSSLAM
                     LTARDIVPVNLDNDLRDKNASSETPVHRAIYRALPGIGAVVHSHPPYATALSFDMDEV
                     RTIDSDSLFIPVIPVLKDCPYAEGSACVASGFPRTLDSHKVALVRGHGAFAVGDSLAQ
                     CAARLTMMENQCRLVFFRRLLERR"
     gene            852..1397
                     /locus_tag="P5079_06110"
     CDS             852..1397
                     /locus_tag="P5079_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRY29597.1"
                     /translation="MNKKERLEKIFRMRVGQAEIPDWQFRKYLLLFFVSAAFVFLWTP
                     TRWLIRSWHDFKTFDGLTAPDLSPAVPALGGASGLPGEALLSRDTDRGATAAKTPRAV
                     VFRLLAPAAKSVFLGGSFNGFDARKHALSRRPDGVWEITLDLTPGRYLYKFKVDGRWE
                     LDPTNPDKTPQPRSSSILAIQ"
     gene            1409..3439
                     /locus_tag="P5079_06115"
     CDS             1409..3439
                     /locus_tag="P5079_06115"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HRY29598.1"
                     /translation="MSFGKAVIACALLFSASPLLAMGRARRVPPPGSSSTSPVVSPVP
                     VVEAPSALESVGVVWVPSTEEEVLSGAEFIRANPSFRATVVLPERLFSSEPEKMWPAF
                     QIISSAVVQGQLEPVLDISGNLPLALIQDTELARVSSGPAAALPLRYAWPDDVIGQIA
                     DAQLAFKKRWRRPAHTFRMPAGVFTGTETPMLEKMGIRGVLLTEGPGRPGYFKGLPLG
                     VVRTTVFPDRPQMQALWLSSWISGKGSPVADAAFAGGSTAGGPEVSFLSPVKFTALSA
                     LPAFQGALAAKTTFHWTLISEMLSSAEVFPIWPDAEEPPPDFSPWIGEPEENNAWELL
                     GMTRRAVENYKNSGVANVKALDMAMREIHNAERGEYFYYFGNDYDSSRDPDLEREFLA
                     TLAQVYRLMGQSIPPALLQSFAAATPRVTEQEAGDTTFVRQGSVLRWTDPAKDDRGPG
                     DYFYPTAAEFPPGAWDILSFEMIEQEKTVSFVFEFSAMANPKNAPYGFSGPLVDVYID
                     INHLPGAGNESLLSGRPGLVETQNAWEYALSLDGWGVGVYQYGSGASNRRLAHLPVRK
                     VAVSTVRVDVPKALLRGSPDRWRCAVVVMGHEPGEVSPTARDVPDRPMRVLSEPGPRE
                     FGGAWAGTAYAASDREAPPFIDLIVPPQQTQSQVLSIYKQGREVVLPFLSPE"
     gene            3499..4941
                     /locus_tag="P5079_06120"
     CDS             3499..4941
                     /locus_tag="P5079_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY29599.1"
                     /translation="MCAGLCAAAPQEPEPSLNSRAAGYQEMFVRVAAAVKPCVVNITT
                     LQDGSDGIFSDDEFDYFPYPEDDFFGAPPSSRRPADPSRRRYQGMGSGVLISTDGYIL
                     TNEHVVHGALSMEVTVLSPVEKVYVGRVAGLDSRTDLAVIKINPKEKLPCVELADSDK
                     VRVGDWALAVGSPFGLEQTVTAGIVSAVRQSLTIEGHTYSDLFQTDAAINRGNSGGPL
                     MNLDGRLIGINTAIYAPTGVFSGVGFAIPSNRAREVMTQLIEKGRVVRGWMGVEIIPL
                     DDVLAGQFGVPDARGVVVNDVLPDSPAAAAGLRRGDVLRKFNGQEVLSLESLTAVVGR
                     TPPKTTVEVVFLRDGKTERTGLLTGEMPPDPSDRAVLPPQEAPRRARTPTGTWGGARW
                     GDLTPALARKYALPRDRKGVVALRVEADGLVERIGLLPGDLVVSINRRESPDVETFVR
                     MMNEVSLKEGVLLDVNRRGRWLYLSHREGE"
     gene            4946..5866
                     /locus_tag="P5079_06125"
     CDS             4946..5866
                     /locus_tag="P5079_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29600.1"
                     /translation="MDARVLLVEDDPELMEFVRFMLEQEKYRVITASDGEDGLNKGRT
                     ERPDIILLDVLLPKIHGFEVCERLRQDPATCLIPIIMVTSLTAIKDRLTGIKLGADEY
                     VSKPFEPVELLARVENLIKRTRQNVAANPLTGLAGDVAFQQELHRRLQEKTEFSVAYA
                     DANGLSDYNKLYGFEKGDGIIRLLGTIMRSAAVELGNRNDMVVHLGGDDFALISTPAR
                     AEVIAARILENVEYLMPMQYDEKERERGYAFLHESAGKEKKHPFLTFSIGLVDVMPDL
                     YQHHAQILDRARAALKEAKSKGGNQLARLT"
     gene            5880..6872
                     /locus_tag="P5079_06130"
     CDS             5880..6872
                     /locus_tag="P5079_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HRY29601.1"
                     /translation="MNIQNRLIAAFFAMLGVLLAGVLGYHFIEGWNLFDALYMTVITL
                     ATVGYGETNPLSPTGRMFTIFLIFGGIGLLTYAFSTFTAFLVEGELGEALRRRRMQKK
                     IDSISNHYIVCGGGRTGSYIIDEFLKVKTPFVVIDLNPDEAAALSQRGVLVLTGDASH
                     DDVLRQAGIDRARGLITCLPTDKDNMFVVVSAKGINPRLRVVAKSVDKGVREKFFRGG
                     ADSVVSPNFIGGLRMASGMLRPAVVSFLDTMLRDTESTLRVSEATVGGRAAGKTLEET
                     VHRLGLDLVVLAVRAASGQDYAFNPKPKTRLVQDDVVVVMGPVDEVHKLQQALA"
     gene            complement(6891..9155)
                     /locus_tag="P5079_06135"
     CDS             complement(6891..9155)
                     /locus_tag="P5079_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29602.1"
                     /translation="MTRRDIHLLLWFGGGSLLLFFLPLFAGETHFSGDILYSFHPWFT
                     YAAQNIQSGEFPLWNPFSACGESFIGNPQIMLFYPGALFFWTFPFPVGWTSFLAFHQW
                     LLFTATGLAVRSFYKTNLRTTSVLLAAVSMTWGGFVTVLWEFPGAVASLPFLLLFFIL
                     GLTRRWLSFSLSLSLLLLAGYTPFAYYAVLLGAAGMGVQVFFRRGDRRSPDWKTLLFW
                     SAALGAGIAACAPQILSTWEAARHSLRAMISPSEARNYLLTPIFLIKFLVPEIFDKVA
                     LPFQSHAFGAEFWPVQRNWLNTFFIGTLPLLLALAALTDFRKRKNLFWGAVILFSAAM
                     AMGWDPVFSAARTLIPGFRYMTHFSHLILLTVVSLVFLAADGGEAPARKPLVFWGLWG
                     GAFLLVIALAAFPTCRLFTLKKLLGTALLSPAQDRHVAGAAAKTAVILLGAGALALCP
                     ARVRGWGFVFLTLAELRFFAIGFHPTVSMDFFKEPVTLQEKLQAAPGRYAVDPETVRG
                     PKFMAGETLAAGYQSLRQALYPNIQLPFRIRSTWGYEVFPEKNFSEFRRNIPSDNLGG
                     APARFLGIDHVLSTRPLPPPAELLARAPHALLYSFDDPLPLVGWVPLSRKIADPQERL
                     SFLSSPWDPTKEVILEEEESVSAGIAPVPGKPLRRLKERSSYLEVAGEGAGWVVRGET
                     FHSGWNVYVNGQHRRLMRANHAFQAAKTPPGGWTAVFLYRPKMFRRGLGIGLAALLGL
                     GFGAVLFMRRTALR"
     gene            complement(9160..>9505)
                     /locus_tag="P5079_06140"
     CDS             complement(9160..>9505)
                     /locus_tag="P5079_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29603.1"
                     /translation="FLNVLGETEPGCSVKADDKSMSVDSEGRFTGRLYLREGKNRFVF
                     ASRDGSGNEVKTERVVYRLLPGQKGKFQKAQRPEEEKEEEGRAKSGALTSIAVGLLTI
                     GTIVAIFLLIIQ"
BASE COUNT         1800 a         2803 c         3042 g         1860 t
ORIGIN      
        1 ctccttttgc ggcgtttccg acggaaccgt cggggctttg aggtgcggcg ctacgtcgtg
       61 accggcggcg aaacggcgtt ccggttcgtc cgtctgctgg ggcgcggctt ctggcgtgtg
      121 gcggaccgcg tggaacgggg cctgcctctg tgcgtggaag acatccagcg ctctccgcgc
      181 cgccggccct gttggatggt tctgaaaccc ggcggttttg ggagtgaaac ggcgttgata
      241 aaaagtgttc ggaggcttca gcgtgttctc tgattttttc acgtacggac gtgtgctttg
      301 ggaggcggga catctccacg tcacctccgg caatatgtcg gtgaagaagg ggagcgtcat
      361 ctacgtcacc cgtaccggca gttccctggc gatgctgacg gcccgggaca tcgtgccggt
      421 caatttggac aacgatctgc gcgacaagaa cgcctcttcg gagacgcccg tgcatcgcgc
      481 gatctaccgg gcgttgccgg gcatcggcgc cgtggtccat tcgcacccgc cctacgccac
      541 cgcgctgtcg ttcgacatgg acgaggtgcg gaccatcgat tcggacagtc tttttattcc
      601 cgtcatcccc gtgctgaaag actgccccta cgccgagggg agcgcttgcg tggcttccgg
      661 gttccctcgg acgctggatt cgcacaaggt cgcgctggtc cgcggccatg gcgcctttgc
      721 cgtgggcgac agcctcgcgc agtgcgcggc gcgcttgacg atgatggaga atcagtgccg
      781 cctggttttt ttccggcggc ttttggagcg ccgctgagag gcggcggcat aatcgaaggg
      841 aggtggcggc gttgaacaaa aaagagaggc tcgagaaaat attcaggatg cgtgtggggc
      901 aggcggaaat acccgattgg cagttccgga agtatcttct cctttttttc gtttcggcgg
      961 cctttgtttt cctttggacg cccacgcgat ggctgatccg cagttggcac gattttaaaa
     1021 cgttcgacgg tctcaccgct ccggacttgt ctccggcggt ccccgcgctc ggaggggcct
     1081 ccggtttgcc gggggaagcg cttctgagcc gggataccga ccggggcgcg acggccgcca
     1141 aaacgccgag agccgttgtt tttcgcctgt tggctcccgc cgcgaagtcc gttttcttgg
     1201 gggggagttt caacggtttt gatgcccgaa aacacgcgct ctcccgccga ccggatgggg
     1261 tctgggagat cacgctcgat ttgactcccg gccggtatct ctataaattc aaagtggatg
     1321 gtcgttggga acttgatccc accaaccccg ataagacccc acaacctcgt tcgagttcca
     1381 tcctcgccat ccagtaggga tgtctagagt gtcgttcggt aaagcggtca tcgcctgcgc
     1441 tttacttttt tccgcatctc cgctcctcgc catggggcgc gctcggcggg tgccgccccc
     1501 cggttcctcc tcgacctccc ccgtcgtctc tccggtcccc gttgtggaag ctccttccgc
     1561 cctggaaagc gtcggtgtcg tttgggtccc ctccaccgaa gaggaagtcc tttcgggggc
     1621 cgagttcatc cgggccaacc cgtctttccg cgccacggtg gttttgccgg aacggttgtt
     1681 cagcagcgaa ccggaaaaaa tgtggccggc ttttcagatc atttccagcg ccgtggttca
     1741 gggacaattg gagccggtcc tggatatttc agggaacctg ccgctggcgc tgatccaaga
     1801 cacggagttg gcccgggtct catccggccc ggcggcggcc ttgccgctgc gctatgcctg
     1861 gccggacgac gtcattggcc agatcgccga tgcccaattg gcgttcaaga agaggtggcg
     1921 ccgtccggcg catacgttca ggatgcccgc cggggtcttt accgggacgg agacgcccat
     1981 gttggagaag atggggatcc ggggagtcct cctgacggag ggccccggcc gccccggcta
     2041 ttttaaaggt ttgccgctcg gcgtcgttcg gaccacggtt tttccggacc gcccgcagat
     2101 gcaggctttg tggctttcct cctggatctc cggaaaaggg tcgccggtcg ccgacgccgc
     2161 gtttgcggga ggctcgacgg ccggcggacc ggaggtgtcg tttttgtctc cggtgaaatt
     2221 caccgccctc tccgctttgc ccgcgttcca gggagcgctg gccgccaaga cgacgttcca
     2281 ttggacgttg atttcggaga tgttgtcttc ggccgaggtg tttcccattt ggccggacgc
     2341 ggaggaaccc ccgcccgact tttccccttg gatcggagaa ccggaagaga ataatgcctg
     2401 ggagctgttg ggcatgaccc ggcgcgccgt tgagaactat aaaaattcgg gggtggcgaa
     2461 cgtaaaggcg ctggatatgg ccatgcgcga gatccataac gccgaacggg gggagtattt
     2521 ctattatttc gggaacgact acgattcgtc ccgggatccc gacttggaac gggaattctt
     2581 ggcgacattg gcccaggtgt atcgcctcat gggacagagc atccccccgg cccttttaca
     2641 aagttttgcg gccgccactc cccgcgtcac ggaacaagag gcgggcgaca ccacgtttgt
     2701 gcggcaaggc tctgtgttgc gctggacgga ccccgccaaa gacgatcgtg gtccggggga
     2761 ttatttctat cccacggccg ccgaatttcc gccgggcgct tgggacatcc tttcgttcga
     2821 aatgatcgag caggaaaaga ccgtttcttt tgtattcgaa ttttcggcca tggccaaccc
     2881 caagaacgcc ccttacggtt tcagcggccc gttggtggat gtttacatcg acatcaacca
     2941 tctgccgggc gcggggaacg aatctttgct ctcgggtcgg cccggcctgg tcgaaactca
     3001 gaacgcctgg gaatacgcct tgagcttgga cggctggggt gtcggcgttt atcaatatgg
     3061 ttccggcgcc tccaatcgtc ggttggcaca tctgccggtt cggaaagtgg cggtctccac
     3121 cgttcgcgtg gatgtcccca aggccctgct ccgcggcagt ccggaccgat ggcgatgcgc
     3181 ggtggtcgtc atgggacatg agccggggga ggtctccccc accgcgcggg atgtgccgga
     3241 ccgccccatg cgcgttttga gcgagccggg accgcgtgag ttcggcggcg cctgggccgg
     3301 caccgcttac gccgcctccg accgcgaggc cccgccgttc atcgatctca tcgttccgcc
     3361 ccagcagacg caaagccagg tcctcagcat atacaagcag gggcgggaag tcgttctccc
     3421 gttcctgtct ccggagtgaa cgtgtttttg gggaacactc cggcgcggcg gtcgattttt
     3481 tccggaggag gtcttttgtt gtgcgccggg ctttgcgccg ccgcccccca ggagccggag
     3541 ccttctctga attccagggc cgcgggatac caggagatgt tcgtccgggt cgcggccgcc
     3601 gtcaaaccgt gcgtggtcaa catcaccact ctccaggacg gctcggacgg cattttttcc
     3661 gacgacgaat tcgattattt tccttatccg gaggacgatt ttttcggcgc tcctccatct
     3721 tcccgtcgtc ccgccgatcc ttcccggcgg cgctaccagg gaatgggctc cggcgtcctc
     3781 atcagcaccg acggctacat cctgacgaac gaacacgtgg tccacggggc cttgtccatg
     3841 gaagtgacgg tcctttcccc cgtcgaaaag gtctatgtcg gacgggtggc cggactcgac
     3901 tcccgcacgg atctggccgt gatcaaaatt aatcccaaag aaaaacttcc gtgcgtcgag
     3961 ctggcggatt cggacaaggt ccgcgtgggc gattgggccc tggcggtggg cagcccgttc
     4021 ggactggaac agacggtcac ggccggcatc gtctcggccg tccgccaatc cctcaccatc
     4081 gaaggacaca cctacagcga tttgttccag acggacgcgg ccatcaaccg gggaaattcc
     4141 ggagggccgt tgatgaactt ggacggacga ttgatcggca tcaacaccgc catctacgcc
     4201 cccacgggcg tgttctccgg ggtggggttc gccattccgt cgaaccgggc ccgggaagtc
     4261 atgacccagc tcatcgaaaa aggacgggtg gtgcgcggct ggatgggcgt cgagatcatt
     4321 ccgctcgacg acgtcctggc cggccagttc ggcgtccccg atgcccgggg ggtcgtggtc
     4381 aacgacgtct tgccggattc tcccgccgcg gcggcggggc ttcggcgcgg ggacgtcctc
     4441 cgaaagttca acggacagga agtcctttcc ctggaatcgt tgaccgccgt cgtcggccgc
     4501 acgccgccca agaccacggt ggaggtcgtt ttcctgcgtg acggaaaaac ggagcggacg
     4561 gggctcctca cgggcgagat gccgcccgat ccgtccgacc gggcggtttt gcctccccaa
     4621 gaggcgcccc gccgcgcacg cactcccacg gggacgtggg gtggggcccg gtggggggat
     4681 ttgaccccgg ccctggcccg gaaatacgcc ttgccccggg atcgcaaggg ggtggtcgcc
     4741 ctccgcgtcg aggcggacgg gttggtcgaa cggatcggcc tgctgccggg ggatctggtc
     4801 gtttccatca accgaaggga atccccggat gtggaaacat ttgttagaat gatgaacgag
     4861 gtttcactga aggaaggtgt gctgttggat gtgaatcggc gtggccgctg gctttatctc
     4921 agtcaccggg aaggagaata accgcttgga cgcccgcgtt ttgttggtgg aggacgatcc
     4981 cgaactgatg gagttcgtcc gcttcatgct ggagcaggaa aaataccgcg tcatcaccgc
     5041 ctccgacggc gaggatggtc tcaataaggg gcggacggaa cgccccgaca tcatcctcct
     5101 ggacgtcctg ctgcccaaga ttcatgggtt cgaggtgtgc gaaaggttgc ggcaggatcc
     5161 cgcgacctgc ctgatcccca tcatcatggt gacctccctc acggccatca aagaccgcct
     5221 gacgggcatc aagttggggg ccgacgaata cgtctcaaaa ccttttgaac cggtggaact
     5281 gctggcccgc gtggagaacc tcatcaaacg gacgcggcag aacgtggcgg ccaacccgtt
     5341 gacggggctg gccggtgacg tggcttttca gcaggaactt catcgccggc tccaggaaaa
     5401 aacggaattt tccgtggcct acgcggacgc caacgggctg agcgactata acaaactcta
     5461 cggtttcgag aagggggacg ggatcatccg ccttttggga accatcatgc gcagcgccgc
     5521 ggtggagttg gggaatcgga acgacatggt ggtccacttg gggggagacg atttcgccct
     5581 catcagcacg ccggcgcgcg cggaagtcat cgcggcgcgc atcttggaga acgtggaata
     5641 cctcatgccc atgcaatacg acgagaagga aagagaacgt ggctacgcct tcctgcatga
     5701 aagcgccggg aaagagaaga agcatccctt tttgacgttc tccatcggct tggtggatgt
     5761 gatgcccgac ctataccagc atcacgcgca gatattggat cgcgcccgcg ccgccctgaa
     5821 agaggccaag tccaagggcg gcaatcagct cgcccgcctt acttgaaaaa atcccttacg
     5881 tgaacattca aaacaggttg atcgcggcgt tcttcgcgat gctgggcgtt ttgctggccg
     5941 gcgtcctggg ataccatttc atcgaggggt ggaacctctt cgatgccctg tatatgacgg
     6001 tcatcaccct cgccacggtg ggatacggcg aaacgaatcc actgtcgccc accggacgga
     6061 tgttcacgat tttcctgatt ttcggcggga ttggccttct gacctacgct ttttcgacgt
     6121 tcacggcatt tttggtggaa ggagaattgg gggaagccct acggaggaga cgcatgcaaa
     6181 aaaagattga ttccatctcc aatcattaca tcgtttgcgg cggcggtcga acggggagct
     6241 atatcataga tgaatttctg aaggtcaaga cgccgttcgt ggtgatcgac ctgaaccccg
     6301 acgaggcggc ggcgctctcc cagcgggggg ttctcgtgtt gacgggcgac gccagccacg
     6361 acgacgtgct ccgtcaggcc ggcattgacc gggcgcgcgg cctcattacc tgcctgccga
     6421 ccgacaagga caacatgttc gtggtcgtca gcgccaaggg catcaatcct cgcctgcggg
     6481 tcgttgcgaa gtccgtggac aagggggttc gggaaaagtt tttccggggc ggagctgatt
     6541 ccgtggtgtc ccccaacttc atcggtggtc tgaggatggc ttcgggaatg ctgcgcccgg
     6601 ccgtcgtttc tttcctggat accatgcttc gggatacgga atcaacgttg cgcgtttcgg
     6661 aggcgaccgt gggcggccgc gcggccggaa agactttgga agagaccgtg caccgtttgg
     6721 ggttggacct cgtcgtcctg gcggtccggg cggcttcggg acaggattat gcttttaatc
     6781 caaaacctaa aacaagactg gtccaggacg atgtggtggt cgtcatgggg ccggtcgacg
     6841 aggtccacaa gctgcaacaa gcgttggcgt gaccgtttcc agaagatctt tcaacggagc
     6901 gcggtccgtc gcatgaacag gaccgccccg aaccccaacc ccaacagcgc cgccaggccg
     6961 atccccaacc ccctccggaa catcttcggc cgatagagaa acaccgccgt ccagccgccc
     7021 ggcggcgttt tcgccgcctg gaacgcatgg ttggcccgca tcaatcggcg gtgttgtccg
     7081 ttcacgtata cgttccatcc ggaatgaaat gtttccccgc gcacgaccca gcccgcgcct
     7141 tccccggcga cttccaaata agaagacctt tcctttaggc gccggagcgg ttttccggga
     7201 acgggggcga ttccggccga tacgctctct tcctcctcca ggatcacctc ttttgtcggg
     7261 tcccacggag acgaaagaaa cgacaatcgt tcctgcggat cggcgatttt tcgggagagg
     7321 gggacccagc cgaccaacgg caagggatcg tcgaaagaat agagaagggc atgcggggcg
     7381 cgggcgagga gttccgccgg cggcggcagg ggccgggtgc tgaggacgtg gtctattccg
     7441 aggaaacgcg ccggcgcccc gccgagattg tccgacggga tgtttctccg gaattcggag
     7501 aaattttttt ccggaaagac ctcatacccc cacgtggaac gtatgcggaa aggcagctgg
     7561 atgttgggat agagcgcctg tctcaggctc tgataccccg cggccaatgt ctcgcccgcc
     7621 atgaatttcg ggccgcgcac ggtttcggga tccacggcat accgccccgg tgcggcctgc
     7681 agcttctcct ggagagtcac gggttccttg aaaaagtcca tcgagacggt cgggtggaag
     7741 ccgatcgcaa aaaatcgaag ttcggcgagc gtcagaaaaa caaatcccca gccgcggacc
     7801 cgggcagggc aaagggcgag agcccccgcc ccgagaagaa taacggcggt ttttgccgcc
     7861 gcccccgcga cgtgcctgtc ctgagcgggg gacagaaggg ccgtgccgag gagctttttc
     7921 aatgtgaaca gccggcaagt cgggaaggcg gccagggcga tcacaagcaa aaaagcgccg
     7981 ccccacagtc cccaaaagac caggggcttc cgcgcggggg cttctccgcc gtccgcggcc
     8041 agaaagacca gcgacacgac ggtcaggagg atcagatggg agaaatgcgt catgtagcgg
     8101 aacccgggga tcagcgtgcg ggcggcgctg aaaacggggt cccatcccat ggccatggcc
     8161 gccgagaaca ggatgacggc gccccaaaag aggttctttc gtttacggaa atccgtcagc
     8221 gccgccagtg cgagcaggag agggagggtc ccgatgaaaa aagtgttgag ccagttccgt
     8281 tgcaccggcc agaactccgc gccgaacgcg tgcgattgga agggcagggc gaccttgtcg
     8341 aatatctccg gaacgaggaa tttgataagg aagatgggcg tcaataaata gtttcgcgcc
     8401 tcggaagggc tgatcatcgc gcggagggag tgtcgtgcgg cttcccaggt cgacaggatc
     8461 tgaggggcgc acgcggcgat ccccgcgccg agggcggcac tccaaaagag gagggtcttc
     8521 cagtcgggag agcggcggtc cccccggcgg aaaaaaacct gaacccccat cccggccgcc
     8581 ccgaggagga cggcatagta ggcgaaagga gtgtagccgg ccagaagaag cagggagagg
     8641 ctcagtgaaa aactcagcca tcgccgtgtc agcccgagga tgaagaaaag gaggaggaac
     8701 ggcagggagg cgacggcgcc cgggaattcc cacaagaccg tcacaaaacc tccccacgtc
     8761 atcgagacgg cggccagaag aacgctcgtc gtacggaggt tcgtcttgta aaaggatcgg
     8821 accgccagcc cggtggccgt gaagaggagc cattggtgga acgccaggaa actcgtccag
     8881 ccgacgggaa aggggaaagt ccagaaaaac agggcgccag gatagaacag catgatttgc
     8941 ggattgccga tgaaactttc tccacaggcc gagaacggat tccaaagggg aaattcgccg
     9001 ctttggatgt tttgggcggc ataggtgaac caggggtgga aggaataaag gatgtcccct
     9061 gaaaaatgtg tttctccggc aaaaaggggg aggaagaaga gcaacaggga acctcccccg
     9121 aaccacagga ggaggtggat gtctcggcgc gtcattcggt cattggatga tgagcaaaaa
     9181 gatggcgacg atggtgccga tggtgagcaa tccgacggcg atggaggtga gggccccgct
     9241 cttggcgcgt ccctcttctt ctttttcctc ctccggccgt tgcgctttct ggaatttccc
     9301 tttttggccc ggcaataaac ggtagaccac gcgttccgtc ttgacctcgt tgccgctgcc
     9361 gtcccgcgag gcgaagacga agcggttctt gccttcccgg agatagagac gtcccgtgaa
     9421 acgcccttcg gaatcgacgg acatcgactt gtcgtccgct ttcacggagc aaccgggttc
     9481 cgtttcgccc agcacgttca ggaat
//
LOCUS       DAOTFD010000079         9502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5105_length_9502_cov_3.979782, whole genome shotgun sequence.
ACCESSION   DAOTFD010000079 DAOTFD010000000
VERSION     DAOTFD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5105_length_9502_cov_3.979782"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1748)
                     /locus_tag="P5079_06145"
     CDS             complement(54..1748)
                     /locus_tag="P5079_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29604.1"
                     /translation="MATPPPSSPMTDSAPRVKKASSLLRRTAFVLLTLLPLAAYRHWT
                     VQDLPKQGLHYDVALVGVNSLGLYRGFTPNDRRDNDLFVGPAPLMFNRYNGAVECYLG
                     ALPALFVFGATPYALNLAGVSWGFVFLLLCGLAGWVWFENRWAAPVVLTLLGTLPSFA
                     AFSRIGLYAGTVHGSLFAAAVLFFLIYWKNPGKNWAFPAAAVFMGLALGSRTLMVWYF
                     AALALTAAMNAPLRRRIAELPAPHKIFSAAALCSGPLLVFLANARHGWFTFGFFSKFS
                     LHSRSGVDNLAYGANLLERGREFLTLWNASAWTNINRPNRLAAALLIAAVLLTIVDLI
                     SRKKNLPRPPAAGLWPLLVCLLVFALSPFTPTFLDVHHLSALYCPAALLLASPLFGPP
                     AGPGKKYVRFLFAAGLLAYASVNVSLFRQFQPHRNVHGGTEVKWDVMTEVIAWLQKQG
                     IHRLGLGDTGISDPFVFLAGGGIQADEIFHAYYFEDAREKREGLLRQRLRQEKTGFYL
                     FREPEIERIPFHDRFLEIVASEGKRITPEARFPAPDGTPVFVVYRVEDKPPPRERV"
     gene            complement(1765..3153)
                     /locus_tag="P5079_06150"
     CDS             complement(1765..3153)
                     /locus_tag="P5079_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29605.1"
                     /translation="MKIALLITPTLFKRCPLIGVGSLTAYLRSLAHDIVPIDLNIATD
                     YPRDAEEGAWQEHSPFVTEYIASNQPMFQKWAEEILSRSPGLIGFSLWNTTLRTSLEL
                     ARHLKKLDDGPILVFGGPQVNFAGPPLLEQPGVDILVTGEGERPLAALAERLERGEEL
                     PGCPGTIAKNDAGRIVQGPPSPEIENLDTLPHADYTDLPLKKYYLSNTLPLSFNRGCQ
                     RRCTFCNIHNDWKSFRSRSARHIFGEMKTQKSRHPDITHFEIDGAALNQDMRQTLDLC
                     DLIIADDFHIGWGGQAFIRPEMGEEAVMEKMARAGCRNLNYGLESGSQKVLNILRKGF
                     TVEHAEKCLRAAHKQGIALGLNVIIGTPGETEEDFQETLSFLSRNRDYLEMLGTPSEL
                     HLSQGTEMTRHPDHFGITPGPTAAEYNWRAGDNTREVRLRRVKEFREFVEREKIQSKH
                     PFQWIKTDVPKN"
     gene            complement(3240..4184)
                     /locus_tag="P5079_06155"
     CDS             complement(3240..4184)
                     /locus_tag="P5079_06155"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY29606.1"
                     /translation="MPPSSPAGPQEKIRRQVAAANLLRLQSLETVLAEFQRAGMPVVL
                     LKGAALLLTAYDHPGDRPMTDVDLWLPFHPVSTAVKMLGRLGYGAVPGRPGAFVHPGD
                     DNDRLDVEHDIWFLNAGDTEMYDRGVETTMGRLSVRTLCPEDAFLYAVAHGLIHHGQW
                     QNKWGEDLRRLTALPKFDWDAVLDRGERWGLTEALLETFTLVPGLAERTTEQRCAELL
                     ARRSSCWKLFLLRRLAATPISGKGHFLRLLMQPRRGRRCSLLFKQLFPSPAFLKQRHP
                     RLPLPAAYALRPFFLFGRFLEFSCRFSAGPRSSGVWRF"
     gene            complement(4190..5713)
                     /locus_tag="P5079_06160"
     CDS             complement(4190..5713)
                     /locus_tag="P5079_06160"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29607.1"
                     /translation="MAKTDTRSQKIRRLIPTLAVCLWLAGPARAARIHPQAGSTAATF
                     LKIAPGARAAAMGEAFGALADDASALYWNPAGILQLRHKEFTAMHDESFQDIRYGFVG
                     YAQPWGERRAWGVSASGLFLQNDLERRSGVGESVPETPLTPSEGTFGADDTSLAVSGA
                     FYPWRALAFGATLKGIRQRIDDEKAAGAALDLGALYQIPKKPVRVALAFQNIGPGMRF
                     VQRHYKLPFNVKVGGAWQAHPKVDVTLDVNQPIDNYTRFHTGLEYRIFRFFALRAGYK
                     YRLHGNELGEVSGLTAGSGFRFGAAGLDYAFNPAGDLGDTHRLSFTLRFGENPARTPA
                     AVPRKAEQVPAEMEPPREEKIAFRLKLTSLPAATAESPSHRVEAEANTPADLYHLTFQ
                     TVVRSTGSARLESVESPVMDEFPVPAGPVYKMISFHHTFPGGLRKMDAAFRVKKSWLK
                     AVAVAAADVRLARVEGPRRFLKPTRITGENDDYVSFAASMDREGTLAILARPKPLKK"
     gene            complement(5724..8342)
                     /locus_tag="P5079_06165"
     CDS             complement(5724..8342)
                     /locus_tag="P5079_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY29608.1"
                     /translation="MNFFERYQRQRQAAAVLVALLQLTPARAGDWPVFKRDVTRAGHA
                     REQAYPSLSVAWVYDNIQGEVRSSPVVANGRVFIGGRDGGVWAFHPDTGQILWQYSTD
                     GYVDASPAAANGYVYVPSCDGRLYAFAETDGTEPLWTYGTGSQDMSSPAVYQERVYWA
                     TGYPNRQLLAVHARSGELVWQADLSINSLSSPALEDGVLAVGVNNGTIRAFNASDGSP
                     QWAYQTTGRIEFSSLAGDGQGYFYAVAGGDDPHLYAFNARTGQLKTGYPVSLVDISPM
                     GYGKPATETSSVVVADGKVYVAMTGKTGPSTVTLQLFCRNTADGTQAWAPQNLGSAPE
                     GLGYASTPAIANDVLYIGSGDGKLYMFNAVDGTSLGSIALGSAIVSSPAVANGKVFVG
                     TLAGHVYALQAERVAAISSPADGAVVGGTVNISGVVANPAFENYKVEYGVGAAPDTWT
                     AIGEVHTAAVPTNGVLESWDTSALTPQTYTVRLSVAASGAGAASAKSYVTLAIDASYA
                     RQYIVAAAGGTLAANDGTGLAVAAASLNQSDFITLSKPGGVPSGTVPESAAATNVVRQ
                     VAFDSPLTLFLTPASLTVPYQDADVLGLDTAQLRLFYLDGASWKLLENSSVNTSSKTV
                     TGLISQPGYYRILENRLVGTDQDFVYISPDGAEVHVTKGTFSQPDSVTVTKHTAGEYI
                     TAQTPANAQAMPAAWEFKTGKPDTTFAKLVTIKVPYDPSLLGTTPETRLRMYLWYPSE
                     KIWKIVNGSTVEPALRKVTVQVSHFSVYRAMAYLATGVLLEKDKVYTYPNPARGDTVT
                     FKTALGDDAVLTIDVYNVAGERVARLTNSGTAGNVVETVWDVRDVASGVYVYRVEAKG
                     LAGGKADVTKKLAILH"
     gene            complement(8362..>9502)
                     /locus_tag="P5079_06170"
     CDS             complement(8362..>9502)
                     /locus_tag="P5079_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY29609.1"
                     /translation="VFTAVAPPAPPIGSGWPQSLGTGTIADAIQTMPVTDGVSIYVGS
                     NNGKFYALNPDGTPKWTVPFDAGASVLGTPVAGFEPNYIYVVTSGGRLLKVKDTGAAG
                     STASQDGGWIRDIGAVGSAQYVLTSSTNIYVGTSSNQMRKLRAGDGTDVWPAPPQLLG
                     QVNGTVAVDETTPGVNELWVGDAGGGVSRIKTNDGTILSSFTSVGNVPINSDPLILAG
                     YSYAPNNKDFLYITAGSRVYLRNRFNLKTSTDTWSDYDTGGAEITTSVEVDGFFEGST
                     PHLYFGTQDGRLIKLDGLTGSEIWSFQAPAAIESTPFFNEDDGYVYFGCNDGKVYAIR
                     YSDKLQKPNWPYNAGAAVRSSLSMSSGNLLVGSDDGKLHVLDITP"
BASE COUNT         1597 a         2842 c         3338 g         1725 t
ORIGIN      
        1 cgttttaagg cgggggccgg tcccgtcgaa ccatcttttc aagagagcgg tcttcatacg
       61 cgttcccgcg gcgggggctt gtcctcgacg cgatagacga cgaacaccgg ggtgccgtcg
      121 ggagccggaa aacgcgcctc gggggtgatc cgtttcccct cggaagcgac gatttccagg
      181 aaacggtcgt ggaacgggat gcgttcgatc tccggctcgc ggaagaggta gaaaccggtc
      241 ttttcctggc gcagtctttg gcggagaagc ccctcgcgtt tctcccgggc gtcttcaaaa
      301 tagtaggcat ggaatatttc gtccgcctga atgccccctc cggccaaaaa aacaaaaggg
      361 tcgctgatcc cggtgtctcc cagtccgagg cggtggatgc cctgtttttg aagccaggcg
      421 atgacctccg tcatgacgtc ccatttcacc tccgtcccgc cgtgcacgtt gcggtggggt
      481 tggaactgcc ggaaaagaga aacgttgacg gacgcatagg ccagcagtcc cgccgcgaag
      541 aggaaccgga catatttttt ccccggtccc gccggcggac cgaacagcgg ggaggccagg
      601 agcagggcgg cgggacagta caacgccgac aggtggtgca cgtcgaggaa cgtcggcgtg
      661 aaaggcgaaa gggcgaagac gagcaggcag accagcagcg gccagagtcc ggcggcggga
      721 ggacggggaa ggtttttctt gcgggaaatc agatcgacga tcgtcaggag caccgccgcg
      781 atcagcagag cggccgcgag gcggttcggg cggttgatgt tggtccacgc cgaggcgttc
      841 cacagggtca ggaattcccg gccgcgttcg aggaggttgg cgccgtaggc cagattgtcg
      901 accccgctcc gtgaatgaag ggagaatttg gaaaagaaac cgaaggtgaa ccatccgtgg
      961 cgcgcgttgg ccaagaagac cagcagcggt ccggagcaga gggcggcggc ggaaaagatc
     1021 ttgtgcggcg cgggcagttc ggcgatccgc cgacggagcg gcgcattcat ggcggcggtc
     1081 agcgccaggg cggcgaagta ccacaccatc agcgtgcggc ttcccagcgc caggcccatg
     1141 aaaacagccg ccgcgggaaa cgcccagttt ttccccgggt ttttccagta aataaggaaa
     1201 aacaacacgg cggcggcgaa gaggcttccg tgcaccgtcc ccgcgtacag cccgatgcgg
     1261 ctgaacgccg cgaaactcgg caaggtcccc agcagggtca gcacgaccgg cgccgcccag
     1321 cggttttcga accagaccca tccggccagc ccgcaaagga ggaggaagac gaatccccag
     1381 gaaacaccgg cgaggttcag ggcgtagggg gtggcgccga agacaaacag ggcggggagc
     1441 gctcccaggt aacattcgac cgcgccgttg tatcggttga acatcaaagg ggccgggccg
     1501 acgaacaggt cgttgtcgcg ccggtcgttc ggggtgaaac cgcggtaaag tcccagggag
     1561 ttgacgccca ccagggccac gtcgtaatgc agtccctgtt tcgggagatc ctgaacggtc
     1621 caatgccggt aggccgccag gggcagcaag gtcaaaagaa cgaacgcggt ccgacggagg
     1681 agggacgacg cttttttgac gcgcggggcg ctgtcggtca tcggggagga tggcggtggt
     1741 gtcgccatga agggtttccg cggctcaatt tttcgggacg tccgttttta tccattgaaa
     1801 gggatgcttc gactggattt tttcccgttc cacgaattct cggaactctt tgacgcgccg
     1861 gaggcggact tcgcgggtgt tgtcccccgc ccgccagttg tattccgcgg cggtgggccc
     1921 cggggtgatg ccgaaatggt cgggatgccg ggtcatttcc gtgccctggg agaggtggag
     1981 ttcgctgggg gtcccgagca tctcaagata gtccctgttg cgcgacagga aggagagggt
     2041 ctcctggaaa tcttcctcgg tctcccccgg ggttccgatg atcacgttca gtccgagggc
     2101 gatcccctgt ttgtgcgcgg cgcggaggca cttttcggcg tgttccaccg tgaacccctt
     2161 gcgcaggatg ttgagcactt tctgggagcc gctctccagg ccgtagttca aattgcggca
     2221 gccggcgcgg gccatttttt ccatcaccgc ctcctccccc atttccggcc ggatgaaggc
     2281 ctgtccgccc cagccgatgt ggaaatcgtc ggcgatgatc aggtcgcaca ggtccaatgt
     2341 ctggcgcatg tcctggttca gcgcggcgcc gtcgatctcg aaatgggtga tgtccggatg
     2401 gcgtgatttc tgggttttca tctcgccgaa aatatggcgg gcgctgcggg aacggaaact
     2461 tttccagtcg ttgtggatgt tgcagaacgt gcatcgccgc tggcagccgc ggttgaacga
     2521 gaggggcagc gtgttggaaa gataatattt tttcaagggc aggtcggtgt agtcggcgtg
     2581 cggcagcgtg tcgaggtttt cgatctccgg gctggggggg ccctggacga tgcgccccgc
     2641 gtcgttcttt gcgatcgtgc ccggacagcc ggggagttcc tccccgcgtt ccaaccgttc
     2701 ggcgagggcc gccaggggac gttcgccttc ccccgtcacc aggatgtcca cccccggctg
     2761 ttccagcagg ggcggtcccg caaaattcac ctgcggcccg ccgaaaacga ggatggggcc
     2821 atcgtcgagt tttttgaggt gccgcgccaa ttccaagctc gtccgcagcg tggtgttcca
     2881 gagactgaaa ccgatgagcc ccggcgaacg ggagaggatc tcctccgccc atttttggaa
     2941 catgggttgg ttggacgcga tgtattccgt cacgaaaggg gagtgttcct gccaggcccc
     3001 ctcctcggcg tcgcgggggt agtccgtggc gatgttcaaa tcgatgggga cgatgtcgtg
     3061 ggcaagggac cggagatacg ccgtcaagga accgaccccg atgagcgggc atctcttaaa
     3121 gagggtcggc gtgatcagga gcgctatttt catgggtcgg acggttcctt ctgggactca
     3181 cagtcctggg acggtcggtt ttttccggcg gtgaacatcg gaatgatacg taatccgttt
     3241 caaaagcgcc agacgccgga cgaacgcggt cccgctgaaa agcggcagga gaattctaaa
     3301 aaccggccga aaaggaaaaa gggacgtaac gcataggcgg cggggagggg cagacgggga
     3361 tggcgttgtt tcaggaaagc gggcgatggg aacagttgtt tgaacaacaa agaacatcga
     3421 cgcccccggc gcggttgcat aaggagccgc aggaagtggc ctttgccgga gatcggcgtg
     3481 gcggccagtc gccgcaggag gaaaagtttc cagcaggagg aacgccgggc gaggagctcg
     3541 gcgcatcgtt gctccgtcgt ccgctcggcg aggccgggga cgagggtgaa cgtttccagg
     3601 agggcttcgg tcaaccccca gcgctcgccc cggtccagaa cggcgtccca gtcgaacttc
     3661 gggagggcgg tcaggcggcg caggtcttcc ccccacttgt tctgccactg gccgtggtgg
     3721 atcaagccgt gcgccacggc ataaaggaag gcgtcctcgg ggcacagcgt ccggacggac
     3781 aggcggccca tcgtcgtttc aacgccgcgg tcgtacattt cggtgtcccc ggcgttcagg
     3841 aaccagatgt cgtgttccac gtccagccgg tcgttgtcgt cgccgggatg gacgaaggct
     3901 cccggacgtc cgggcacggc gccataaccc agcctcccca gcattttcac cgccgtcgac
     3961 accggatgga acggaagcca gaggtcgacg tccgtcatgg gccggtcgcc gggatggtcg
     4021 taggccgtca gaaggagggc cgctcccttg agcaggacga cgggcatccc ggcgcgttga
     4081 aattccgcca acacggtttc gagggactgc agacgaagga ggttggccgc cgccacctgg
     4141 cgtcggattt tttcctgcgg gccggccggg gaggacgggg gcatgggatt tattttttga
     4201 ggggtttcgg ccgggccagg atggccaggg tgccttcccg gtccatcgac gcggcgaaac
     4261 tcacgtagtc gtcgttctct ccggtgatgc gcgtgggttt gaggaaccgc cgcgggcctt
     4321 ccacccgcgc caggcgcacg tccgcggcgg cgacggcgac ggccttcaac caggattttt
     4381 tcacccggaa ggccgcgtcc atttttcgca gcccgccggg gaaggtgtgg tggaaggaga
     4441 tcattttata cacgggcccc gccgggacgg ggaattcgtc catcaccggc gactcgacgg
     4501 attccaaccg cgcggacccg gtgctccgca cgacggtttg gaaggtcaga tggtagagat
     4561 cggccggcgt gttggcttcg gcctccacgc ggtgggacgg cgattcggcc gtggccgcgg
     4621 ggagcgaggt cagtttcagg cggaacgcga tcttctcctc ccggggcggt tccatttcgg
     4681 cggggacctg ttcggctttc cgggggacgg ccgccggcgt gcgggccgga ttttccccga
     4741 aacgcagggt gaacgagagg cggtgcgtgt cgccgagatc cccggcgggg ttgaaggcgt
     4801 aatcgagccc ggccgcgccg aagcggaacc cgctgcccgc ggtcagcccc gacacctcgc
     4861 ccagttcgtt gccgtgcagg cggtacttgt atccggcccg aagggcgaag aaacggaaga
     4921 tccggtattc gaggcccgtg tggaaacggg tgtagttgtc gatgggctgg ttgacgtcca
     4981 gcgtgacgtc gactttgggg tgggcctgcc aggcgcctcc caccttcacg ttgaagggaa
     5041 gtttgtaatg gcgctggacg aacctcatcc ccggtccgat gttctggaag gcgagggcca
     5101 cgcggaccgg ttttttcggg atttgataga gcgctcccag gtccagcgcc gcgccggcgg
     5161 ctttttcgtc gtcgatccgc tggcggatcc ccttcagcgt ggcgccgaag gccagcgcgc
     5221 gccagggata gaaagcgccg ctgacggcca gcgaagtgtc gtcggcgccg aaggtccctt
     5281 ccgacggcgt gagcggcgtt tcggggacgg attcccccac gccgctgcgg cgttccaggt
     5341 cgttctgcaa gaacaggccg gaggcgctca cgccccaggc gcgccgctct ccccacggtt
     5401 gggcgtagcc gacgaaccca tagcggatgt cctggaacga ttcatcgtgc atggcggtga
     5461 attccttgtg ccgcagttgc agtatcccgg ccgggttcca atagagggcc gaggcgtcgt
     5521 cggccagcgc gccgaaggcc tctcccatgg cggcggcccg tgcgccgggg gcgatcttca
     5581 agaaggtggc cgcggtgctc cctgcctgcg gatggatgcg cgccgcccgg gccgggccgg
     5641 ccagccagag gcagacggcg agcgtcggga tcagccggcg gattttttga gaccgcgtat
     5701 cggttttagc catagtgtct tcctcagtgc aggatcgcca gcttttttgt gacgtccgct
     5761 tttcctcccg ccagcccctt cgcctccacg cggtagacgt acacgccgga agccacgtcg
     5821 cggacgtccc acaccgtttc gacgacgttg ccggcggtgc cgctgttggt gaggcgggcg
     5881 acgcgttccc ccgccacgtt atacacgtcg atggtcagga cggcgtcgtc gccgagcgcg
     5941 gtcttaaagg tgacggtgtc cccccgggcc gggttgggat aggtgtagac cttgtccttt
     6001 tccaaaagga cgcccgtcgc caggtaggcc atggcgcggt acacggaaaa atggctcacc
     6061 tggacggtca ccttgcggag ggccggctcc accgtggacc cgttgacgat tttccagatt
     6121 ttctccgacg gataccagag gtacatccgc aggcgcgtct cgggcgtggt ccccagcagg
     6181 gaggggtcgt acggcacctt gatggtgacg agtttggcga aggtggtgtc cggtttgccc
     6241 gtcttgaact cccatgccgc cggcatggcc tgggcgttgg ccggcgtctg ggcggtgatg
     6301 tattcccccg cggtgtgttt ggtgacggtg acggaatccg gctgggagaa ggtgcccttg
     6361 gtcacgtgca cctcggcgcc gtcgggcgag atgtagacga aatcctggtc ggtccccacc
     6421 agccggtttt ccaggatgcg gtaatagccc ggctgggaga tgagccccgt caccgtcttg
     6481 gagctggtgt tgacggagga gttctccagc aacttccagg aggcgccgtc caggtagaag
     6541 agccggagct gcgccgtgtc gagcccgagc acgtcggcgt cctggtaggg cacggtgagg
     6601 gaggccggcg tcaaaaagag ggtgagcggg ctgtcgaagg cgacctgccg caccacgttg
     6661 gtggcggcgg cgctttcggg gaccgtgccg gacggcacgc cgcccggttt ggagagggtg
     6721 atgaaatcgc tttggttcaa cgaggcggcc gccaccgcca ggccggtgcc gtcgttggcg
     6781 gcgagcgtgc cgcccgcggc ggcgacgatg tattgccggg cgtaagaggc gtcgatggcc
     6841 agcgtcacgt aggatttggc gctggcggcg cccgcgcccg aggccgccac gctcagccgc
     6901 acggtgtagg tctggggcgt cagggccgag gtgtcccagc tttccagaac gccgttggtc
     6961 ggcacggcgg cggtatggac ctcgccgatg gcggtccagg tatcgggcgc cgcgccgacg
     7021 ccgtattcca ccttgtaatt ttcgaacgcc gggttcgcca ccacgccgga gatgttcacg
     7081 gttcccccca ccacggcgcc gtcggcggga gaggaaatgg ccgccacccg ttccgcctgc
     7141 agcgcgtaca cgtgaccggc cagggtgccg acgaagacct tgccgttggc caccgccgga
     7201 ctgctgacga tggccgaacc cagagcgatg gatccgagcg aggtgccgtc gaccgcgttg
     7261 aacatgtaga gctttccgtc gccggagccg atgtagagca cgtcgttggc gatggcgggc
     7321 gtggaggcgt accccaggcc ttccggcgcg ctgccgaggt tttgcggcgc ccacgcctgg
     7381 gtgccgtcgg cggtgtttcg gcagaaaagt tgcagcgtca ccgtgctggg gccggtcttt
     7441 ccggtcatgg ccacgtacac ctttccgtcg gccaccacca ccgaggaggt ctccgtggcg
     7501 ggtttgccgt aacccatggg gctgatgtcc accagcgaga cgggatagcc ggttttcagc
     7561 tggccggtcc gggcgttgaa ggcatagagg tgaggatcgt ccccgccggc cacggcgtag
     7621 aaatagccct ggccgtcgcc cgccaaggag gagaattcga tccggccggt ggtttgatag
     7681 gcccattgag gagaaccgtc ggaggcgttg aaggcgcgga tggtgccgtt gttcacgccg
     7741 acggccagca cgccgtcctc cagcgccggg gaggagaggc tgttgatgga caggtccgcc
     7801 tgccaaacca gttctcccga ccgggcatgc accgcgagga gctgtcggtt gggatagccc
     7861 gtggcccaat agacccgttc ctggtacacc gccggggagg acatgtcttg gcttccggtg
     7921 ccgtaggtcc aaagcggctc ggtgccgtca gtttcggcga aggcgtacag ccggccgtcg
     7981 caggagggca catagacgta tccgttggcc gcggccgggg aggcgtccac gtagccgtcg
     8041 gtggagtatt gccagaggat ctgtccggtg tccggatgga aggcccacac gccgccgtcc
     8101 cgcccgccga tgaacacgcg gccgttggcc accaccggag aggagcggac ctccccctgg
     8161 atgttgtcgt agacccacgc caccgagagg ctggggtagg cctgttcgcg ggcgtggccg
     8221 gcccgggtga cgtcgcgctt gaacaccggc cagtcgccgg cccgtgccgg cgtgagttgc
     8281 aggagggcga cgaggaccgc cgccgcttgc cgctgtcttt gatatctttc gaaaaagttc
     8341 attttatccc ccgctctttt cctacggcgt gatatccaac acgtgcaatt tcccgtcgtc
     8401 ggatcccacc aggaggtttc cgctgctcat gctgagcgag ctgcgcaccg cggcgccggc
     8461 gttgtaaggc cagttcggtt tctggagttt gtcgctgtag cggatggcgt acaccttgcc
     8521 gtcgttgcat ccgaaataga cgtagccgtc gtcctcgttg aagaaaggag tggactcgat
     8581 ggccgccggc gcctggaacg accagatctc cgatccggtg aggccgtcca atttgatcag
     8641 ccggccgtcc tgcgtgccga aatagaggtg cggcgtgctc ccctcgaaga acccgtccac
     8701 ttcgacggac gtggtgatct ccgcgccgcc ggtgtcgtag tccgaccagg tgtcggtgct
     8761 ggtcttgagg ttgaaccggt tgcgcaggta gacccgggat ccggcggtga tgtagaggaa
     8821 atccttgttg ttcggcgcat agctgtatcc ggcgaggatc aacgggtccg agttgatggg
     8881 gacgttgccc accgaggtga aagaggacag gatggtgccg tcgttggtct tgatacggga
     8941 gacgccgccg ccggcgtcgc ccacccacag ttcgttcacg ccgggcgtgg tctcgtccac
     9001 cgccacggtg ccgttcacct gtcccaggag ctggggcggg gccggccaga cgtcggtgcc
     9061 gtccccggcg cgcagtttcc gcatttggtt ggaggaagtg ccgacgtaga tgttggtgct
     9121 gctggtgagg acgtactgcg ccgagccgac cgcgccgatg tcccgaatcc agccgccgtc
     9181 ctgcgaggcg gtggagccgg cggcgccggt gtctttcacc ttcaggagcc gtcctccgga
     9241 ggtgacgacg tagatgtagt tgggctcgaa acccgccacc ggcgtgccca ggaccgaggc
     9301 cccggcgtcg aacggcacgg tccacttcgg ggtgccgtcg ggattgagcg catagaattt
     9361 gccgttgttg gagccgacgt agatgctgac gccgtcggtg accggcatcg tctggatggc
     9421 gtcggcgatc gtgccggtgc ccagggactg gggccacccg ctcccgatcg ggggcgccgg
     9481 cggcgccacc gcggtgaaca cc
//
LOCUS       DAOTFD010000080         9481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5123_length_9481_cov_4.234352, whole genome shotgun sequence.
ACCESSION   DAOTFD010000080 DAOTFD010000000
VERSION     DAOTFD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_9481_cov_4.234352"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2365
                     /gene="pheT"
                     /locus_tag="P5079_06175"
     CDS             <1..2365
                     /gene="pheT"
                     /locus_tag="P5079_06175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRY29610.1"
                     /translation="SPEELAALLRRQGFDVASITRFGGDVQNVVTAKVESVQKHPNAD
                     RLSLCKVFDGKNRLDVVCGAPNVAAGQTVPLARVGARVAGGLEIKRAVIRGQESHGML
                     CSARELGLGDDHGGILILPEDVPLGKDAAAVLAMGDAVLDVEVTPNRPDALSHWGLAR
                     EIAAALKKKVRLPEMRISKAKRDPSLVKIEETTLCTRYVGRALEGVKVSPSPLWMKLR
                     LERCGIRSINNVVDITNYVLLEMGHPLHAFDRDKLKEGRVVVRRAAEGETLLCLDGVT
                     RSLGNHLVIADAAAPTALAGVIGGEPTAVHTGTGRLLLESAHFQAAAVRRARMALAVS
                     TESSYRFERGTDWDMADLASRRAAHLILQIAGGTLLAELDARGKKPPEKKAVKARPAR
                     INLLLGFPIAPAEIKKTLERLGFVVKGSPARFSVLPPMHRHDVKEEADVAEEIARMTS
                     YDKVPTRIRTAPQTPHDPSRENSLVRLSGDFLAAQGFWEAKNYGLLSRAAWEKCRTLP
                     DEATVELANPMSLSGELLAPALLPLLIGNLQTNARRGNKNVRLFECARTFRQADGKIL
                     EPLCLAWVAEGDAQAPHWTHRPRPLDIWDAKAWAKGLFSAWGLDGIEFRPGPGHPFLH
                     PAENLSIHLGGVNVGYLGKLHPRRAEEWELPQNVYAAELDLSFVAAKQPKTSKFAGLP
                     KHPAVFRDFSVVFPEKTPWTAIADRLARHTFVDEIELFDVFKDATLPTGHHSLAFRIA
                     FRDPERTLTDAEVGEIQKSLLADVQSAFGASLRLVGAKNTSSPGENM"
     gene            2362..3114
                     /locus_tag="P5079_06180"
     CDS             2362..3114
                     /locus_tag="P5079_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29611.1"
                     /translation="MNPSGNGTRLRFPFSTARRAGTFVLLLLVLATSGCVYLRLLEIK
                     NQLREFNSNFDTIGDKDLLIQFKKPVLLARDISYLIGAEPLSITLRAKDITWYYEFEI
                     IRSTPLAKPLPLDKLSLSFLLRGGKLVSLTVPENFLRFFPRQMFLETIRQASNAEVNK
                     LRKTVHARLQLSPETDEQLPDIKETVQLLGPPLQKTQEGRLQYYIYRYRIVDCKRDVP
                     IIAKLGFSKRGTFRRLIVLWDTSTLDAEYIRS"
     gene            3092..4282
                     /locus_tag="P5079_06185"
     CDS             3092..4282
                     /locus_tag="P5079_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRY29612.1"
                     /translation="MPNTSGHKKSPVSFLLPLLWLGLPFSSPVFLCAADPLPERPFLK
                     KLSPADWPSFSDDDGLKDLAESCRQSLSYYNTLPPDREFLFGNDRLSVSHMIDTFETL
                     LTLLGDSSPDFRAALEKQFDVYRADGPAPSRDVTFTAYYEHTLDASLSPTAEYRFPLY
                     RRPPDLMELPATSAGGITVGRKNETGEFVPYYSRAEIDTEKILADRGLEIAWAKDPLE
                     IYFLQVQGSGWLQLPRGDRLRIRYQANNGHPYRSIGTFLIERGLLPKENFSRAVMAAY
                     LRNHPDQRQDILNQNPRYVFFEIDRGPQSRHALGSLRVPLTAKRSIATDPQLFPKGAL
                     AWMEIEGDDPVRRFVLNQDEGGAIKGPGRVDYFVGEGPEAETYAQRFYRRGKLYLLVK
                     KKRS"
     gene            complement(4298..5119)
                     /locus_tag="P5079_06190"
     CDS             complement(4298..5119)
                     /locus_tag="P5079_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRY29613.1"
                     /translation="MRVVAVDAAWKTVRRHVAPAPPPAHLGPALQKLFRAWRTKPLKY
                     LTVASKGIWEPAERRSFWKRLRFLAERVWVISDVELAFEGAFTPLRGRPGRSRGILLS
                     AGTGSIAFGRDERGRAARAGGMGPRRGDEGSGYWMGREYLKRRCAARRGYRSGEVARV
                     AAGAKEVLRRARRDQASRKIVREAHRHLARLAFRLAEKLHFGRRIPMSLAGGLFEDVR
                     FRKGFWRTLKRTGRGKVFVCAPSRREPARAAAQWGPFLKGTIEKPFGYELPPRGD"
     gene            complement(5164..6753)
                     /gene="nagZ"
                     /locus_tag="P5079_06195"
     CDS             complement(5164..6753)
                     /gene="nagZ"
                     /locus_tag="P5079_06195"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRY29614.1"
                     /translation="MTVNELIGETLVFGIPGTRVGEEDIRLFRETHAGGLILYRINFV
                     SPAQIRRLITDLETALERRLLVTVDHEGGRVVMFSEGVTVFPDGLAVSLGGRPADARR
                     QGEIEGRELRRLGMDVNFAPVADVLTETHSPNIGIRSYGMDVRRVAAFVAARVRGMRS
                     AGLSACAKHFPGLGPAARDPHLDLPVIRCGWKEMRSKHLVPFEAAIGAGAEMIMSSHP
                     LYPRLDPSPKTPATFSRRLMTDCLRGELGFEGVLASDDLEMGALRRLCPPGRAAVLAS
                     RAGHDLLLSCHKASAQKEIYRALVEAYGNGELSVRTLEDSVERVRRLKEKRSTRFSGG
                     ISPAEREGAILSARISRKAVRILPAGGASGRPKTVNSLTGAKGAAVLTPMSKGTKPNV
                     LIVFPRLSEVADIIMVEKELRREARFLRDTLKRVKERIRTMVLPLNPPASAIRRAAEA
                     AGRSDRTVYFCQDAHLYEGCKKMLAVLGRASRRLTVVLLRDPYDVALAPPRSAVVSSF
                     GYRACQIRACLEKLFGPGMER"
     gene            6836..7726
                     /gene="murQ"
                     /locus_tag="P5079_06200"
     CDS             6836..7726
                     /gene="murQ"
                     /locus_tag="P5079_06200"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRY29615.1"
                     /translation="MKDFSSLPTEQANPASRDLDRLPLKKLISLMNREDARPAQAVRR
                     VMPDIARAVGLIVGALKKGGRLFLVGAGTSGRLCVMEAAECPPTFNTPPEWVQALMAG
                     DKAAVFRSREGAEDNALEAERRIRRKLKAGDVTVGVAASGVTPFVLSALMETRRRRGH
                     TVLVTCNPNAGQRSLAEVVIAPAVGPEILAGSTRLKAGTACKMVLNMLTTLSMVRLGK
                     VYRHWMVDLQPKSLKLKARGTRLIQLLGRVSEKEAANYLKKARGRVKTAIVMARNGIS
                     RLEAVSRLRRRGGFLHKVLK"
     gene            7761..8966
                     /locus_tag="P5079_06205"
     CDS             7761..8966
                     /locus_tag="P5079_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRY29616.1"
                     /translation="MNQILRKSLFAALGLFLAGGVLYAQTESGLDVLESQNFSPLRGK
                     KVGLLCNHTALDRRGRPAAELFNRAPGVELKALFSPEHGFSGRLQHGQPIPDDYYAGV
                     PVYSLYGKTKRPTAEMLRDLDALVFDIQDVGARFYTYIATLGYALEEAARHGLEFYVL
                     DRPNPLGGDVAEGEPLAEDIRHFTAYYSIPTRHGLTVGEIAQWHNAHARLGAKLTVVR
                     MKGWKRKNLWKDTGRRFVPPSPNIRSPKAALLYCGIGAFEATNVSVARGTKTPFELFG
                     APWMDGPRLADRLNALALPGIKFKAARFTPRSDLYAGLSCGGVRLLVMDESAVRPVDV
                     FIHAVCLLRELHAAEFQPRWPEMPYVVGSRDFETLYKTGEPADQILQRLHAGAAEFLK
                     TRLPYLLYE"
     gene            8991..>9481
                     /locus_tag="P5079_06210"
     CDS             8991..>9481
                     /locus_tag="P5079_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29617.1"
                     /translation="MMVEWFAGGSRLTLADFSVLGALLTLMVLIGTFFRRQQRSTHDF
                     FLGGRKVPWWAACLSFVATEVSAVTIISVPAVAYMENWEYLQFFIGSAAARLIIAYLF
                     IPAFYRFDCTTIYQFLGHRFGTLTQAAASFFFFVTRLLGSGVRLMAACLAMSVLLGWD
                     IVPA"
BASE COUNT         1805 a         3179 c         2781 g         1716 t
ORIGIN      
        1 gtccccggag gaactggccg ccctgctccg tcggcagggg tttgacgtcg cctccatcac
       61 ccggttcgga ggggacgttc aaaacgtggt cacggccaag gtggagtcgg tccagaaaca
      121 tcccaacgcc gaccggctct ccctctgcaa agtcttcgac ggaaaaaacc gtctcgacgt
      181 ggtctgcggc gcccccaacg tggccgcggg acaaacggtc ccgctcgccc gcgtgggcgc
      241 gcgcgtggcg ggagggcttg agatcaaaag agccgtcatc cgcggccagg agtcccacgg
      301 aatgctctgt tccgcccgcg aactggggct gggggacgac cacggaggga tcctgatcct
      361 gcccgaagac gttccgctcg gcaaggacgc ggcggcggtg ttggccatgg gcgacgcggt
      421 gctcgacgtg gaggtgaccc ccaaccgtcc ggacgccctg tcccactggg gcctggcgcg
      481 agagatcgcg gccgccctga agaaaaaagt ccgtctgccg gagatgagga tctccaaagc
      541 caaacgggac ccgtccctcg tcaaaataga ggagacaacg ctttgcacgc ggtacgtcgg
      601 acgggcgctc gaaggggtga aggtctcccc gtcgccgctt tggatgaagc tgcgcctgga
      661 acgctgcggc atccgatcca tcaacaacgt ggtggacatc accaattacg tcctgctgga
      721 gatgggccat ccgctccacg cgttcgaccg ggacaagctc aaggaaggtc gcgtggtggt
      781 ccgccgggcc gcggaagggg agacgctgct ctgtctggac ggcgtcaccc gttccctcgg
      841 aaaccatctc gtcatcgcgg acgccgcggc cccgacggct ttggcgggcg tcatcggcgg
      901 agaaccgacc gcggtccaca cgggaacggg ccggctcctt ctggaaagcg ctcattttca
      961 agcggcagcc gtgcgccggg cccgcatggc ccttgccgtc agcacggaaa gctcctaccg
     1021 gtttgaacgg ggcaccgatt gggacatggc cgacctggcc agccgccggg ccgcccattt
     1081 gatcctccaa atcgccgggg ggacgctcct ggccgaactc gacgcccggg ggaaaaagcc
     1141 gccggagaaa aaagccgtca aagcgcggcc ggctcgcatc aacctcctcc tgggcttccc
     1201 catcgcgccg gcggagatca aaaagacgct ggaacgactg ggcttcgtcg tgaagggctc
     1261 gcccgcccgc ttttcggtcc tccctcccat gcatcgacac gatgtgaaag aagaagccga
     1321 cgtcgccgag gagatcgccc ggatgacgag ctacgacaag gtcccgacgc gcatccgaac
     1381 ggcgccgcaa acgccccacg acccctcccg ggagaactcg ctggtgcgcc tcagcgggga
     1441 ttttctggcg gcccagggtt tctgggaggc caagaactac gggcttctgt cccgcgcggc
     1501 atgggaaaaa tgccggaccc ttccggacga ggccaccgtc gaactcgcca accccatgtc
     1561 cctttccgga gaacttctcg ccccggccct cctgccgctg ttgatcggga accttcagac
     1621 gaacgcgcgg cgggggaaca agaacgtccg cctcttcgaa tgcgcccgga cgttccgaca
     1681 ggcggacggc aaaatcctgg aacctctctg tctggcctgg gtggccgagg gagacgcgca
     1741 ggcgccccac tggacccatc ggccccgtcc gctggacatt tgggatgcca aggcctgggc
     1801 gaaaggtctt ttttcggcgt gggggctgga cgggatcgaa ttccgtccgg gaccgggcca
     1861 tccctttctg catccggcgg agaacctgtc gatccatctc ggcggggtca acgtgggata
     1921 cctcgggaag ctccatcccc ggcgggcgga ggaatgggaa ctcccgcaga acgtctacgc
     1981 cgccgaactg gacctttcgt ttgtggcggc gaaacagccg aaaacatcaa aattcgccgg
     2041 gctgcccaaa catccggcgg tcttccgtga tttctccgtc gtcttcccgg aaaaaacccc
     2101 ttggacggcc atcgccgacc ggctcgcgcg tcatacattc gtcgacgaaa tcgaactctt
     2161 cgacgttttc aaagacgcca ctctgccgac cggacaccac agcctggcgt tccgcatcgc
     2221 tttccgcgat cccgaacgga ccctgacgga cgccgaggtg ggggagatcc agaaaagtct
     2281 gctggccgac gttcagtccg ctttcggcgc ctccctgcgc ctcgtcggcg ccaagaacac
     2341 atcctctccg ggagagaaca tgtgaatccc tccgggaacg ggacgcgcct tcgtttccct
     2401 ttctcaaccg cccgccgcgc cggaacgttc gtcctccttc tcctcgttct tgcgacctcc
     2461 ggctgcgtct acctgcgcct gttggagatc aaaaaccagc tccgcgagtt caactccaat
     2521 ttcgatacca tcggcgacaa agacctcctt atccaattca aaaaacccgt tctcctagcg
     2581 cgcgacatct cctacctgat cggggccgag ccgctcagca tcacgctgcg cgccaaggac
     2641 atcacctggt attacgaatt cgagatcatc cgctccaccc cgctcgccaa accgttgccg
     2701 ctggacaaac tctccctctc ttttcttttg cgcgggggga aattggtaag cctgacggta
     2761 cctgaaaatt tcctccggtt cttcccgcgc caaatgttcc tggaaacgat ccggcaggcc
     2821 tccaacgcgg aggtcaacaa actccggaaa accgttcacg cccgtctcca gctctcccct
     2881 gaaacggacg aacaactgcc cgacatcaaa gagaccgtcc aacttctggg gccgccgctc
     2941 caaaaaacgc aggaagggcg gctgcagtat tacatctacc gatatcgcat cgtcgattgc
     3001 aaacgggacg tccccatcat cgccaaattg gggttttcca aacggggaac gttccgccgc
     3061 ctcatcgtgt tgtgggacac ctccaccctg gatgccgaat acatccggtc ataaaaaaag
     3121 tcccgtttcc tttctcctgc ccctcctgtg gctgggcctc ccgttctcaa gccccgtctt
     3181 cttgtgtgcc gccgatcctc tccccgaacg ccccttcctg aaaaaattat cgccggcgga
     3241 ttggccgagc ttctcggacg acgacgggct gaaggacttg gccgaatcct gccgccaaag
     3301 cctctcctac tacaacacat tgcctccgga ccgggaattt ctgttcggca acgaccgtct
     3361 ttccgtttcc cacatgatcg atacgtttga aacgctgctg acgcttctcg gcgactcctc
     3421 tccggatttt agggcggcgc tcgagaaaca attcgacgtc taccgggccg acgggccggc
     3481 gccttcccgc gatgtcacgt tcaccgccta ctacgagcac acgctggacg cctctttgtc
     3541 cccgaccgcg gagtatcgct tcccgctcta tcgccgcccc cccgatttga tggaactccc
     3601 cgccacgtcc gccggcggga taacggtcgg acggaaaaat gaaacagggg agttcgtccc
     3661 ttactactcc cgggcggaaa tcgacacgga aaaaatcctg gcggaccggg ggctggagat
     3721 cgcctgggcc aaggaccctt tggagattta cttcttacaa gtgcaggggt cgggctggct
     3781 ccaattgccg cggggagacc gtctgcgcat ccgctatcag gccaacaacg gccatcccta
     3841 ccgttccatc ggcacgttcc tgatcgaacg aggtctcctt cccaaagaga atttcagtcg
     3901 ggccgttatg gccgcctacc tccgaaatca cccggatcag cggcaggaca tcctcaatca
     3961 aaacccccgg tatgttttct ttgaaatcga tcgcggcccc cagagccgcc atgccctggg
     4021 aagcctgcga gtgcccctca cggccaaacg ttccatcgcc acggacccgc aactatttcc
     4081 gaaaggcgcc ttggcgtgga tggagatcga gggggatgac cccgtgcgtc ggttcgtcct
     4141 caatcaggat gaaggggggg ccatcaaggg cccggggagg gtcgactatt ttgtgggaga
     4201 aggcccggaa gccgaaacgt acgcccaacg gttttatcgg aggggaaaac tttatctgtt
     4261 ggtgaagaaa aaaagaagct agcggctccg gaagggttca gtcccctcgc ggcggcagtt
     4321 cgtatccgaa cggtttctct atcgtccctt ttaaaaatgg cccccactgg gcggcggcgc
     4381 gggcgggttc ccgccgagac ggcgcgcaca cgaaaacctt cccgcggccg gtccgtttca
     4441 gcgttcgcca aaaacctttt ctgaaacgaa catcctcaaa caacccgccc gccaaactca
     4501 tcgggatccg tcgtccaaaa tggagtttct ccgcaaggcg gaacgccaga cgggccaggt
     4561 ggcggtgtgc ttcccgaacg atcttgcggc tggcctggtc tcgccgggcg cgccgcaaaa
     4621 cttccttcgc ccccgcggcc acccgggcca cctccccgga tcgatatcct cgccgggcgg
     4681 cacaacgtct cttcaaatat tcccttccca tccaataacc ggacccttcg tctccccgcc
     4741 gggggcccat tcctcccgcc cgggccgccc ggccccgttc gtcgcggccg aaggcgatgg
     4801 aaccggtgcc cgccgacaac aaaatgcctc tcgatcgtcc cggccggccc cgcaacgggg
     4861 tgaatgcccc ttcaaacgcc agttccacgt cggagatcac ccacaccctc tccgccaaga
     4921 agcggagtcg tttccaaaac gaccggcgtt cggccggttc ccaaatcccc ttggaggcca
     4981 ccgtcaaata tttcaggggc ttggtccgcc acgcccgaaa cagtttttga agtgcgggac
     5041 cgagatgcgc gggcggcggg gccggagcga cgtgccgccg gacggtcttc caggcggcat
     5101 cgaccgccac gactcgcatt cgagaaccgc ccacatccac accgacggaa taaatctctt
     5161 tcctcatctt tccattcccg gaccgaaaag tttctccagg catgcccgga tctggcaggc
     5221 acggtatccg aaagaggaca ccaccgcgct ccgcgggggg gccaacgcga catcgtaggg
     5281 gtcgcggagg aggaccaccg tcagacgccg agacgccctc cccagaaccg ccagcatttt
     5341 tttgcagcct tcgtacaggt gggcatcctg gcaaaaatag accgttcgat ccgatcttcc
     5401 ggcggcttcg gccgcccggc ggatcgccga cgccggcgga ttcaacggca aaaccatcgt
     5461 ccttattctt tccttgaccc ttttcaacgt gtccctcagg aaccgggctt ctcgacgaag
     5521 ctccttttcc accatgatga tgtccgccac ttcggaaagg cggggaaaaa cgatcaagac
     5581 gttcggtttc gttcccttcg acatcggagt gaggaccgcg gccccttttg cgcctgtcaa
     5641 agaattcacc gtcttcggcc gcccggacgc gccgccggcg gggaggatcc gaaccgcttt
     5701 tcgtgaaatc cgcgccgaca aaatcgcgcc ttcccgttcc gcaggggaaa tccctcctga
     5761 gaatcgggtg gaacgcttct ctttgagccg gcggacgcgt tccacgctgt cttccaaggt
     5821 tcggacggag agttccccgt tcccataggc ctccaccagc gcgcgataga tttctttttg
     5881 ggccgaagcc ttatggcaac tcaagaggag gtcgtggccc gcccgggacg ccaacaccgc
     5941 ggcccggccg ggaggacaca ggcggcggag cgcccccatc tccaggtcgt cgctggccaa
     6001 aaccccctcg aagcccaatt cgccgcgcaa acaatcggtc atcaaccgcc gggaaaaggt
     6061 ggccggcgtt ttcggggagg gatcgagtcg gggataaagc ggatgggagg acatgatcat
     6121 ttccgccccg gcgccgatgg ccgcttcgaa agggacgaga tgttttgaac gcatctcttt
     6181 ccatccgcac cgaatcaccg gcaaatccag atgcggatcc cgggcggcgg gacccagccc
     6241 ggggaaatgt ttggcgcagg cgctgagccc ggccgaccgc atgccgcgga cgcgggcggc
     6301 cacgaacgcc gccacacgcc ggacgtccat cccataggaa cggatcccga tgttggggct
     6361 gtgggtctcc gtcagcacgt cggcgaccgg cgcgaaattg acgtccatcc ccaaccgccg
     6421 gagttcgcgg ccctcgatct ctccctgacg gcgcgcgtcc gccggccgcc cgccgaggga
     6481 aacggccagg ccgtccggaa agaccgtgac cccttccgag aacatcacca cccggccccc
     6541 ctcgtggtcc acggtcacca gaagacgccg ttccaaggcc gtttccaagt cggtgatgag
     6601 ccgcctgatt tgggcggggg agacgaaatt gatccgatac aaaatcagcc cgccggcgtg
     6661 ggtctcacgg aagagacgga tatcctcctc cccgacccgt gtgccgggaa tgccgaaaac
     6721 cagagtttcg ccgatgagtt cattcaccgt catagcgttt taccatagca agtcgctcac
     6781 tttcccgcaa gcgagacagt ggacgcatca gcgacgaaag tgttatcctt cgtgcatgaa
     6841 agatttctcc tctctcccaa cggaacaggc gaaccccgcc tcccgggacc tcgaccgtct
     6901 cccgttgaaa aaactcattt ccttgatgaa ccgggaagac gcgcggccgg cgcaggccgt
     6961 gcggcgggtg atgccggaca tcgcgcgggc ggtcggccta atcgtggggg ccctgaaaaa
     7021 aggcggtcgg ttgttcctgg tcggagccgg gaccagcggc cggttgtgcg tgatggaggc
     7081 ggccgagtgc ccgcccacat tcaacacgcc tccggaatgg gtgcaagccc tcatggccgg
     7141 cgacaaagcc gcggttttcc gctcgcggga aggcgccgaa gacaacgccc tggaagccga
     7201 gcgacggatc cgccgaaaac taaaagcggg cgacgtcacc gtcggagtgg cggccagcgg
     7261 cgtcaccccg ttcgtcttat ccgcattgat ggaaacccgg cgccggcgcg gacacaccgt
     7321 cctggtgacc tgcaacccca acgccggaca acgttccctg gcggaggtgg tcatcgctcc
     7381 ggccgtcggg ccggaaatac tcgccggatc cacgcgactg aaagccggca ccgcctgcaa
     7441 aatggttctg aacatgctca ccacgctgtc catggtccgg ctcggcaagg tctaccggca
     7501 ttggatggtg gacctccagc ccaaatccct caaattgaaa gcccgcggca cacgccttat
     7561 ccaattattg ggacgggtct ccgaaaaaga ggcggcgaac tacctgaaaa aagcccgcgg
     7621 ccgcgtgaag accgccatcg tgatggcccg caacggaatc tcccgcctgg aagccgtttc
     7681 ccggcttcgc cgccgcggcg gttttctcca caaagttttg aaatgacccc ctgccgcacc
     7741 gccaccgatc ttccccgtcc gtgaaccaaa tcctccgaaa atccctcttc gccgccctcg
     7801 gcctctttct ggccggcggc gtcctgtacg cgcaaacaga aagcggcctg gatgtcctgg
     7861 aatcacagaa cttctccccg ttgcggggga aaaaggtcgg ccttctctgc aaccacaccg
     7921 cgctcgaccg gcgaggccgg ccggcggcgg aactgttcaa ccgggcgccg ggggtggaat
     7981 tgaaggccct tttttcgccg gaacacggtt tttccggacg actccagcac gggcagccga
     8041 tccccgatga ctattacgcc ggggttcccg tttatagtct ttatggaaag accaaacggc
     8101 cgacggcgga gatgctgcgg gatttggacg cgctggtctt cgacattcaa gacgtcggag
     8161 cccggttcta cacctatatc gccacgctgg gatacgcctt ggaagaagcc gcccggcacg
     8221 gcctcgaatt ctacgtcctg gaccgcccca atcccctggg gggagacgtg gccgagggag
     8281 aaccgctggc cgaagacatc cgccacttta cggcgtatta ttccatcccc actcggcacg
     8341 gtctgaccgt gggggaaatc gcccaatggc acaacgccca cgcccgcctc ggcgcgaagc
     8401 tcaccgtggt gcgcatgaag ggatggaaga ggaagaatct ctggaaagat accggccgtc
     8461 ggttcgtgcc gccctctccg aacatccgat cgccgaaagc ggcgctcctt tattgcggca
     8521 tcggggcttt tgaagcgacg aacgtttcgg tggcccgcgg gacgaaaact ccctttgaac
     8581 ttttcggcgc cccatggatg gacggacccc gcctcgccga tcggttgaac gcgctggccc
     8641 tgccggggat caagttcaag gcggcgcgtt tcactccccg aagcgacctt tacgcgggtc
     8701 tctcgtgcgg gggcgtccgt cttttggtga tggacgaaag cgccgtccga cccgtggatg
     8761 ttttcatcca cgccgtctgc ctcctccgcg agctccacgc cgcggagttc cagcctcgct
     8821 ggccggagat gccgtacgtg gtgggatcgc gggatttcga gaccctctat aaaaccgggg
     8881 aaccggcgga tcagatcctt caacgcctcc atgccggcgc ggcggaattc ctcaagacac
     8941 gcctccctta tctgctgtac gaatgaggtc cccgggaaaa ggatgaatcg atgatggtgg
     9001 agtggtttgc gggcggctca cggctgaccc tggccgattt ttcagtcttg ggcgcgctgt
     9061 tgacgctgat ggtgctgatc ggcaccttct tcagacggca acaacgttcc acgcacgatt
     9121 ttttcctcgg cggacggaag gtgccgtggt gggcggcctg tctctccttc gtggccacgg
     9181 aggtgagcgc ggtgaccatc atctccgtgc ccgcggtggc ctacatggag aactgggaat
     9241 acctgcagtt cttcatcggg tcggccgcgg cgcggctcat catcgcctat cttttcatcc
     9301 cggcctttta ccggttcgac tgcaccacca tctatcagtt cctcggccat cggttcggga
     9361 ccctgacgca ggccgccgcc tcgttcttct tcttcgtcac acggctgctc ggctccggcg
     9421 tgcggctgat ggcggcgtgc ctggcgatgt cggtgctgct ggggtgggac atcgtgccgg
     9481 c
//
LOCUS       DAOTFD010000081         9352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5215_length_9352_cov_4.915349, whole genome shotgun sequence.
ACCESSION   DAOTFD010000081 DAOTFD010000000
VERSION     DAOTFD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_9352_cov_4.915349"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="P5079_06215"
     CDS             <1..535
                     /locus_tag="P5079_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY29618.1"
                     /translation="LRRPLVKLPNLDRFLETVAARPGKEKARRGTNVPPRMAVLFGSE
                     KTGLSNRYLDRCHAVLTVPTTGKCPSMNLSQAVAVCCYEFSRGRKELGPSASSGDLGR
                     HRDALPTVEETRRLVEHVRRAFEAADFLSFLPPREQEKKIWRTFLQWKLRRADLALLH
                     GFLRFILKKVSGLSHGK"
     gene            525..1031
                     /gene="folK"
                     /locus_tag="P5079_06220"
     CDS             525..1031
                     /gene="folK"
                     /locus_tag="P5079_06220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRY29619.1"
                     /translation="MGNRTVTAVVGLGSNVGRRLDFLRRATRSLRGTSGVRVRRCSPV
                     YETSPVGPRQRKFLNAVVVLRTSLSPDSLLKTLKRIERELGRRRRRRWGPREIDLDLL
                     FFGALRKRTKSLTVPHPRWMARKFVLRPLSDVAPRFRDPVSGRPIKAFFRELTDPSQR
                     IRLYRVPV"
     gene            1137..1937
                     /gene="panB"
                     /locus_tag="P5079_06225"
     CDS             1137..1937
                     /gene="panB"
                     /locus_tag="P5079_06225"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRY29620.1"
                     /translation="MSKVTTATLLEMKKKGEKIAALTCYDASMARLLNGEEIDVILVG
                     DSVGNVKLGYDNTIPVTMEDMFHHVRAVRRGNSRALLVADMPFLSYENDPKEAVRNAG
                     RLVKEGGAEAVKVEGGLEVVPSIKALVQTNIPVMGHLGLTPQSVLRLGGYRVQGKTTE
                     DAEKMVTQARILEGAGVFALVLEAVPASLAKEISKKVAVPTIGIGAGLECDGQILVLD
                     DLLGLTDGPVPKFVKQYAALRSASSRAVKQFRDDVKKKKFPSPEQTYL"
     gene            1941..2876
                     /locus_tag="P5079_06230"
     CDS             1941..2876
                     /locus_tag="P5079_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY29621.1"
                     /translation="MKNLLAAVLPSIFRMSWILLGLFLLFVVLWGWYSSNLVITMERS
                     PLSVIPAAFGLAYEETTFPAPDGVVLKGWFVPAAKPSDTTIVLCHGWGADRANLLPST
                     VFLNQRGGYNLCYFDFRNHGESGGTRSSLGPLEIGDMEAALDYITRAKPEQTRRLGVY
                     GLSMGAAVALTVTARDPRIEAVVAESAFSSFNGVVARFARIFYGVPRLPFVPMTLFFV
                     RARLGIDPELSSPVRFIDRIAPRPVFLIQGDKDERMPPSEGAALFSRAKEPKTLWTIP
                     GADHGEAAEVAGKEYQDRLLQFYDGVFRKQTSLSR"
     gene            2873..3730
                     /gene="panC"
                     /locus_tag="P5079_06235"
     CDS             2873..3730
                     /gene="panC"
                     /locus_tag="P5079_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRY29622.1"
                     /translation="MKIIRSPKQMQKTAWRWRAAGRSVGLVPTMGCFHEGHLSLFRRA
                     RRENDIVVVSVFVNPAQFGPREDFAHYPRPWKRDRRLAGKCGVDVVFHPSAKALYAKG
                     HQTWVDVEELSRPLCGAFRPGHFRGVATVVAKLFQLVQPARAYFGRKDFQQLRIVQKM
                     AADLNFPVRVVPCPTVRERDGLAMSSRNAYLSAEQRRRAAGIPRALQSVGEVIKFSSQ
                     KPISPTVILRKARALLRRIPGARPDYVELVDPRSLRPLKKVAGRALVATAVRLGRARL
                     IDNRLVGRE"
     gene            3825..4637
                     /gene="cdaA"
                     /locus_tag="P5079_06240"
     CDS             3825..4637
                     /gene="cdaA"
                     /locus_tag="P5079_06240"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRY29623.1"
                     /translation="MPLMELLTTLWSKYLIHVADILLVTYIFYRLLLLIRGTRAVQVL
                     RGVVVLAVATFLVQNVFRLPTLSWLFRTFWLAWAVVLAVVFQPELRSVLAQLGSRRLG
                     RLLFPEQLNFINEIISALREASQNRLGMLIVLEQETGLRNFIETGTLINGEVSRDMLL
                     TIFQPRTILHDGAVIVREDRLVAAGCVLPLSNDPDISRILGTRHRAAIGITEISDAVV
                     LVVSEETGVVSVARNGRLDRDVNLDELRDRLRDFYKSMQERGLIRKSGPRDA"
     gene            4639..4785
                     /locus_tag="P5079_06245"
     CDS             4639..4785
                     /locus_tag="P5079_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29624.1"
                     /translation="MNWWRENWQLKLVAFALALFLWIVLYLNPPETDIHSVSWPPHVV
                     ETGP"
     gene            4805..6163
                     /gene="glmM"
                     /locus_tag="P5079_06250"
     CDS             4805..6163
                     /gene="glmM"
                     /locus_tag="P5079_06250"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRY29625.1"
                     /translation="MRKLFGTDGIRGVAGTWPLTPEFVRRLGVVAAKKMRGLSGKPAC
                     LVVRDTRRSGPGLQKALTRGLSAGGLRVYDGGVLPTPAVSALVPRHGFCAGAVLSASH
                     NPAEFNGIKFFGPSGTKLPDALELAMERELPVSPGRMGAVSVPAGSAASSFIRAEDEY
                     LGFLRSAWPLKSDLSGLRLVMDGANGATHRVAGRLFRSLGAEVSALAVSPNGWNINRD
                     CGTLHPQRLAREVLRRRAHVGVAFDGDGDRAMFVDERGRVCDGDAVLLVTSRALKARG
                     RLKKNLVAITVMSNLGLKRAFSALDIDIVETPVGDRYVWQALRDTGAVLGGEPSGHVI
                     FREFLPTGDGLLTALQVLAVLRSSGSPLSALTSLFVRYPQVLVNVKVREKRPLESVRP
                     FWGDVQTVKKSLGEDGRIFVRYSGTEPLLRIMMEGPSQDRIRGYADSLVASARRAGLA
                     SS"
     gene            6183..6917
                     /locus_tag="P5079_06255"
     CDS             6183..6917
                     /locus_tag="P5079_06255"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRY29626.1"
                     /translation="MPLLGVNIDHVATLRQARRVNVPDIQAAAEAAIAGGADNITVHL
                     REDRRHIQDHDVAQLRKNLKVRLNLEMAVTEEMIRVARLVIPHNVCLVPEQRQELTTE
                     SGLDVQAQVSRLRSATKKIKSKGIIVSLFVDPDPVQIQAAKQAGADAVEIHTGAYANA
                     KDAVGRALELERIQSAAQLARDLGLILNAGHGLNYENVRSIAQISGMNELNIGFSILA
                     RAVFVGLEPAVREMKKLINGPAAREE"
     gene            6923..8803
                     /gene="glmS"
                     /locus_tag="P5079_06260"
     CDS             6923..8803
                     /gene="glmS"
                     /locus_tag="P5079_06260"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY29627.1"
                     /translation="MCGIVGYIGGAEASGVLLDGLRRLEYRGYDSAGMATLTDGAFQL
                     RRSVGKLAALESLLGKEPLQGKIGLGHTRWATHGHPSEDNAHPHTDCTGRIVIVHNGI
                     IENYAGLKAELKAQGHRFSSQTDTEVVVHLLEETLKEKTSGRFGKNGSGLDLKTFLSA
                     VRDTLGRLQGAYALGILCSDLPDVLVGARKDCPLILGLGEDEFLLASDVPAILGKTRR
                     AVFLEEGEIAVLTGRSYRVLRQETGEEVQKKVSEIRWDALQAEKSGYRHFMIKEIFEQ
                     PRSVEDTLRSRLELDDWKYYLGNVNISAEQIRALRRISIVACGTSWHAALVGKLLLEK
                     WAKVVCEVDMASEFRYREPLLGPEDLLVVISQSGETADTIAALRYAKERGAHTLAICN
                     VVGSTIAREAHGKIYTHCGPEIGVASTKAFTGQLVVLYLLALYGSFVRGTLPREEAEQ
                     IMEQLLSLPRKIVEVLRLDKKIAALAKKFHQSADFLYLGRNLNYPIALEGALKLKEIS
                     YIHAEGYPAGEMKHGPIALIDEDMPVVAIATRSGVYDKLVSNIEEVKARGGTVIALAS
                     EGDDAIAAHADHVLVVPAVPEFLSPCINVVPLQLLAYHIAVLRGCDVDQPRNLAKSVT
                     VE"
     gene            8835..>9352
                     /locus_tag="P5079_06265"
     CDS             8835..>9352
                     /locus_tag="P5079_06265"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRY29628.1"
                     /translation="MRFFRSLRWNIAIFTLLAALSAAGTFLPQKHDSPERVRFFLSVH
                     PAVGPFLDRLGFFDLYHSAGYVGLLGLLALNVLVCKLRGARNLLSSPIPPTSAPQTVV
                     DVESPLPLLSALNRARGALSARHYRLREERAEGETRLWAFRGLVQRWGDALLHLSVLL
                     ILAGAAYGAAIGA"
BASE COUNT         1588 a         2849 c         3072 g         1843 t
ORIGIN      
        1 gttgaggcgg cccctggtga aactgccgaa tctggatcgt ttcctggaaa cggtcgctgc
       61 ccgccccgga aaagaaaagg cgcgaagggg gacgaacgtc cctccgagga tggcggtact
      121 gttcggttcg gagaaaaccg gtctgtcgaa ccggtatctg gatcggtgtc acgccgtttt
      181 gaccgtgccg acgaccggga agtgcccttc catgaatttg tcccaggcgg tggcggtgtg
      241 ttgttatgaa ttttcccgag ggcggaagga gttgggaccg tcggcctcgt ccggcgatct
      301 cggccggcat cgggacgccc tcccgacggt ggaggagacg cggcggttgg tggaacatgt
      361 gcggcgggcc ttcgaggcgg cggacttcct ttcgtttctg ccgcccaggg aacaggagaa
      421 gaagatatgg cgcacttttc ttcagtggaa actccgccgc gccgacctgg cgctgctgca
      481 cggtttcctg cggttcatcc ttaaaaaggt ctccggtctc tcccatggga aataggacgg
      541 tgacggcggt ggtggggttg gggagcaacg tcggccgtcg gctggacttt cttcgccggg
      601 ccacgcgatc cctgcgggga acgagcggcg ttcgcgtgcg ccgttgttcg ccggtgtatg
      661 agacgtctcc cgtggggccg cgccaaagga agttccttaa tgccgtcgtc gtgcttcgga
      721 caagtttgtc gcccgactcc cttttgaaaa cgttgaaacg gattgaaagg gagctgggcc
      781 gccgccgccg tcgccggtgg gggccgaggg aaatcgatct ggacctttta tttttcggcg
      841 ccctgcgaaa gaggacgaag tccctgaccg tgccgcaccc gcgttggatg gcgcgaaaat
      901 tcgttttgcg gccgctctcg gacgttgctc ctcggttccg cgatcccgtc tccggccgtc
      961 cgataaaggc gtttttccga gaattgacag acccgagcca aaggattaga ctgtatcgag
     1021 tacctgtttg agcgccggcc ttcacgcgct gggaccctcc caagggcctg agacgagggt
     1081 gaacggcccg ccgccggacg aggccgcccg atccgtttcc gaccaaggag ttttccatgt
     1141 ccaaagtgac caccgccacg ctgctggaaa tgaaaaagaa aggcgaaaag atcgccgctt
     1201 tgacgtgtta cgacgcgtcc atggcccgtc tcttgaacgg ggaggagatc gacgtgatcc
     1261 tcgtcgggga ttccgtcgga aacgtgaaac tcggatacga caacaccatt cccgtgacga
     1321 tggaggacat gttccatcac gtgcgggccg tgcgacgggg caacagccgg gcgctgttgg
     1381 tggcggatat gccttttttg tcctatgaga acgaccccaa ggaggccgtc cgtaacgccg
     1441 gccggttggt gaaagagggc ggggcggagg cggtgaaggt ggaggggggg ctggaagtgg
     1501 tgccttccat caaggcgttg gtgcagacca acatccccgt gatgggccat ctgggtttga
     1561 cgccgcagtc tgtcctccgc ctcggcgggt accgcgttca gggaaagacg accgaggacg
     1621 cggaaaagat ggtcactcag gcccgcatcc tggaaggggc gggcgtcttc gcgctggtgt
     1681 tggaggcggt gccggcctcc ctggcgaagg agatctcgaa aaaagtcgcc gtgcccacca
     1741 tcgggatcgg cgcggggctg gaatgcgacg gacagatcct cgtgctcgac gaccttctcg
     1801 gcttgacgga cgggccggtc cccaagttcg tgaaacaata cgccgccctg cgttccgcct
     1861 cttcccgcgc ggtcaaacag ttccgcgacg acgtgaagaa aaagaagttc ccatcccccg
     1921 aacagaccta cctttgatgc gtgaaaaatc ttctggccgc cgtccttcct agtatctttc
     1981 gcatgagctg gatattgctc gggctgtttc ttttgttcgt ggtgttgtgg ggttggtatt
     2041 cctccaacct cgtcatcacc atggaacgtt ctccattgag cgtcatcccg gccgcgttcg
     2101 ggctggccta tgaggagacg acgtttcccg caccggacgg cgtggtgctc aagggatggt
     2161 tcgtcccggc ggccaagccg tcggatacca cgatcgtcct gtgtcacggc tggggggcgg
     2221 accgggccaa cctcctgccg tccaccgttt tcctcaatca gcgcggggga tacaacctgt
     2281 gctatttcga tttcaggaac cacggcgaga gcggcgggac gcggtcttcc ctcggccccc
     2341 tcgagatcgg ggacatggag gcggcgctgg attacatcac gcgggccaag ccggaacaga
     2401 cgcgccgtct gggagtctac ggcctctcca tgggtgcggc cgtggctttg acggtgaccg
     2461 cgcgggatcc ccgcatcgag gcggttgtcg cggaaagcgc tttctcgtcc tttaacgggg
     2521 tggtggcccg cttcgcccgg atcttctacg gcgttccgcg cctccccttc gttccgatga
     2581 cgctgttctt cgtccgcgcc cggctgggca tcgacccgga gctttcctcg ccggtgcggt
     2641 ttatcgaccg aatcgctccg cggccggtgt tcctgatcca gggggacaag gatgaacgga
     2701 tgccgccctc ggagggagcg gcgctgttct cccgcgcgaa agagcccaag accctttgga
     2761 cgatcccggg cgcggaccac ggcgaggcgg ccgaggtggc cgggaaggaa taccaggacc
     2821 gccttcttca attttacgat ggagtttttc gcaagcagac ctcgctgtcc cgatgaagat
     2881 catccgttct ccgaagcaga tgcagaaaac ggcctggcgc tggcgtgcgg ccggccgctc
     2941 cgtcgggctt gtgcccacca tggggtgttt ccacgagggg catctctctc ttttccgccg
     3001 cgcccgccgg gagaacgaca tcgtcgtcgt ctctgttttc gtcaatcccg cgcagttcgg
     3061 tccgcgggag gattttgcgc actacccgcg cccctggaaa cgcgaccgac gcctggccgg
     3121 aaaatgcggg gtggacgtcg ttttccatcc gtcggcgaag gcgctctatg ccaagggcca
     3181 tcagacctgg gtcgacgtgg aggaactttc ccggccgctc tgcggggctt tccgtccggg
     3241 ccatttcagg ggggtggcca cggtggtggc caaactcttc caactggttc agccggcccg
     3301 cgcctatttc ggtcggaaag atttccagca gctccgcatc gttcagaaga tggcggcgga
     3361 cctgaatttt cccgtacgcg tcgtgccgtg tcccaccgtg cgcgagcggg acggactggc
     3421 gatgtcgagc cgcaacgcct acttgtccgc cgaacagcgt cgccgggcgg cgggaattcc
     3481 ccgcgcttta cagtccgtgg gggaagtgat aaaattttcg tctcaaaaac ccatatcgcc
     3541 gacggtgatt ttgcggaagg cccgcgccct gcttcgccgc attcccggcg cccgaccgga
     3601 ctatgtggaa ctggtggatc cgcgttccct gcggcccctt aaaaaggtcg ccggccgcgc
     3661 cttggtggcc acggcggtcc ggttgggacg ggcccggctc atagacaacc ggctggtggg
     3721 aagagagtag accgcatttt taagtctccg gtgaggagga tgttcgtcgg tcatccgcac
     3781 ggttttttca gcgccgcgcc gccgaccttc agaaaacgca cgtgatgccc ctgatggaat
     3841 tgctgaccac gctgtggagc aaatatctca tccatgtcgc cgacatcctg ttggtgacct
     3901 atatcttcta ccggcttttg ctgctcatcc gcggcacgcg ggccgttcag gtgctgcgcg
     3961 gcgtggtggt gctggcggtg gcgacgttcc tggtgcagaa cgtcttccgt ttgcccaccc
     4021 tgagctggct cttccgcacc ttctggctgg cgtgggcggt ggttctggcc gtggtgttcc
     4081 agccggaact ccgttcggtg ctggcccagc tgggcagccg ccggctcggc cggctcctgt
     4141 tccccgaaca gttgaatttc atcaacgaga tcatcagcgc cctccgggag gcctcccaga
     4201 accgcctcgg catgctcatc gtcctcgaac aggagaccgg ccttcggaat ttcatcgaaa
     4261 cggggaccct catcaacggc gaggtgagcc gggacatgct cctgaccatt ttccagccgc
     4321 gcaccatcct gcacgacggc gcggtgatcg tccgggagga ccggctggtg gcggcgggct
     4381 gcgtgttgcc tctttccaac gacccggaca tctcccgcat cctcggcacg cggcaccgcg
     4441 ccgccatcgg catcacggaa atctccgacg cggtggtatt ggtggtcagc gaggagacgg
     4501 gggtggtgtc cgtggcccgg aacgggcgcc tcgaccgcga cgtgaacttg gacgaactgc
     4561 gggaccggtt gagggatttc tacaagtcca tgcaggaacg cggactgata cgtaaatcgg
     4621 gcccgcggga cgcttgatat gaactggtgg agagagaatt ggcagttgaa actggtggcg
     4681 ttcgcgttgg cgctcttctt gtggatcgtc ctctatttga acccgcccga gacggacatt
     4741 cattctgttt cgtggccgcc gcacgtggtc gagaccgggc cctagttttt tccggatagc
     4801 tccgatgcgg aaactttttg ggacggacgg cattcgcggc gtggcgggca cttggccgct
     4861 gaccccggag ttcgtccgcc ggctgggcgt cgtcgccgcg aaaaagatgc ggggactgag
     4921 cgggaagccc gcctgtctcg tggtccggga cacgcgccgt tccggcccgg ggctgcagaa
     4981 ggcgttgacg cgggggttgt cggccggtgg ccttcgggtc tacgacggcg gcgtccttcc
     5041 gaccccggcg gtgtcggcgc tggttccgcg acacggcttt tgtgccgggg cggtcctgtc
     5101 ggcatcccat aatccggcgg aattcaacgg catcaagttt ttcggccctt ccggcaccaa
     5161 attgcccgac gccctcgaac tggcgatgga gagggaactc ccggtttctc cgggacgcat
     5221 gggggccgtg tcggtcccgg cggggagcgc cgcatcgtct tttatccggg ccgaggatga
     5281 atatttgggg ttcctccgtt cagcctggcc gctgaaatcg gatttgtcgg gtctccgcct
     5341 ggtgatggac ggcgccaacg gggccacgca tcgggtggcc ggccggcttt tccgttcgct
     5401 gggggccgag gtgtccgccc tcgccgtctc cccgaacgga tggaacatca atcgcgactg
     5461 cgggaccctg catccccagc gattggcgcg ggaagtcctt cgccgccggg cgcatgtggg
     5521 ggtggccttc gacggcgacg gcgaccgggc catgttcgtg gatgagcggg ggcgcgtctg
     5581 cgacggcgac gcggtgctcc tcgtgacgtc ccgcgcgctg aaagcccgcg gccggctgaa
     5641 gaaaaacctc gtcgccatca ccgtcatgag caacctcgga ttgaaacgcg ctttttcggc
     5701 actcgacatc gacatcgtgg agacgccggt cggcgaccgt tacgtttggc aggcgcttcg
     5761 ggataccggc gccgtcctgg ggggggagcc gtccggacat gtcatcttcc gggaatttct
     5821 cccgaccggc gacgggctcc tcaccgcttt gcaggtgttg gccgttctcc gttcgtcggg
     5881 gagtccgttg tcggcattga cgtccctttt tgtccgttat ccgcaggtcc tcgtcaacgt
     5941 gaaggtgcgg gagaagagac ctttggagtc cgtccgtccg ttctggggcg atgtgcagac
     6001 cgtgaagaag agtctcggcg aggacggacg gatcttcgtc cgctattccg ggacggaacc
     6061 gctcctgcgc atcatgatgg aggggccgtc ccaggaccgg atacggggtt acgcggattc
     6121 tttggtggct tccgcccgcc gcgccggatt ggcgtcgtcg taatataccg tcggagggaa
     6181 aaatgccgct tttgggagtg aacatcgacc atgtggccac gttgcgccaa gcgcggcgcg
     6241 tcaacgtgcc ggacatccag gccgccgccg aggccgccat cgccggaggc gccgacaaca
     6301 tcaccgtgca cctccgcgaa gaccgccggc atatccagga ccacgacgtg gcccagcttc
     6361 gtaaaaatct gaaggtgcgg ctcaacctgg aaatggcggt gacggaagag atgatccggg
     6421 tggcgcggct ggtgataccg cacaacgtct gcctggtgcc cgaacaacgc caggagctga
     6481 cgacggagag cgggctggac gttcaggcgc aggtttcccg gcttcgctcg gcgacgaaaa
     6541 agatcaagag caaagggatc atcgtcagtt tgttcgtcga tccggacccc gtccagatac
     6601 aggccgccaa acaggcgggg gccgacgcgg tggaaatcca taccggcgct tacgccaatg
     6661 ccaaggacgc cgtcggacgc gcgttggaac tggaacggat ccaatccgcc gcccaactgg
     6721 cgcgcgacct cggattgatc ctgaacgcgg ggcacgggtt gaactatgaa aatgtcaggt
     6781 ccatcgcgca gatttccggc atgaacgaac tgaacatcgg attttccatt ctggcccgcg
     6841 ccgtcttcgt cggattggag ccggcggtcc gcgaaatgaa aaaactcatc aacggccccg
     6901 cggcgaggga ggagtaaccg cggtgtgcgg catcgtcggt tatatcggcg gcgcggaggc
     6961 cagcggcgtc cttttggacg gccttcggcg tctcgaatac cgcggttacg attccgccgg
     7021 catggccacc ctcacggacg gcgctttcca gctccgccgc agcgtcggca agttggccgc
     7081 gctggagtcc ctgctgggca aggaaccctt gcagggcaaa atcggcctcg gccatacccg
     7141 atgggccacc cacggccacc cttccgaaga caacgcccac ccgcacaccg actgcaccgg
     7201 ccggatcgtt atcgtccata acggcatcat agagaactat gcgggcctca aggcggaact
     7261 caaggcccag gggcaccgat tttcttccca gacggacacg gaggtcgttg tccacctttt
     7321 ggaggagacg ctcaaggaaa agacatcggg ccgtttcggg aagaacgggt cggggctcga
     7381 tttgaaaacg ttcctgtcgg cggtgcgcga cacgttggga cgccttcaag gggcctatgc
     7441 cctcggcatc ctgtgttcgg acctgccgga cgtcctcgtc ggcgcgcgga aggactgccc
     7501 gctcatcctg ggattgggag aggacgagtt tttgttggcg tccgatgtgc cggcgatcct
     7561 cggcaagacg cgccgggcgg tttttctgga agaaggcgag atcgccgttt tgacgggccg
     7621 ctcctaccgc gtgctccgcc aggagacggg cgaagaggtt cagaaaaaag tttcggagat
     7681 ccgctgggat gccctccagg cggaaaaatc cggataccgg cacttcatga tcaaggagat
     7741 ttttgagcag ccgcggagcg tggaggatac tttgcgcagc cggctggaac tcgacgactg
     7801 gaaatattac ctcgggaacg tgaatatctc cgccgaacag attcgagccc tgcggcgcat
     7861 cagcatcgtg gcctgcggta cgtcctggca tgcggcgttg gtggggaaac tgctgttgga
     7921 gaaatgggcc aaggttgtct gcgaggtgga tatggcgtcg gagttccgct accgggagcc
     7981 ccttctgggc ccggaggacc tgttggtggt gatatcgcag tccggcgaga ccgccgacac
     8041 catcgcggcc ctgcggtacg ccaaggagag gggcgcgcac accctggcca tctgcaacgt
     8101 ggtcggttcc accatcgccc gtgaggcgca cggcaagata tacacccact gcggcccgga
     8161 gatcggggtg gcctccacca aggctttcac gggccagctg gtggttctct acctgctggc
     8221 gctttacggg tccttcgtcc gcggcacgtt gccgcgggaa gaggccgaac agatcatgga
     8281 acagctgttg tcccttccgc ggaagatcgt ggaagtgctg cgcctggaca aaaagatcgc
     8341 cgcgctcgcc aaaaaattcc atcagagcgc ggatttcctc tatctcggcc gaaacctcaa
     8401 ctaccccatc gccctcgagg gcgctctgaa actgaaggag atatcctaca tccacgccga
     8461 gggttacccg gccggcgaga tgaaacacgg gcccatcgcg ctcattgatg aggacatgcc
     8521 cgtggtggcc atcgccaccc ggtcgggcgt gtacgacaag ctcgtgtcga acatcgagga
     8581 ggtcaaggcg cgcggcggca ccgtcatcgc gctcgcctcg gaaggagacg acgccatcgc
     8641 ggcgcatgcc gaccacgtcc ttgtcgtgcc cgccgtgcct gaatttttgt cgccctgcat
     8701 caacgtggtc cctctgcaac tcttggccta ccacatcgcc gtcctgcgcg gctgcgatgt
     8761 ggaccagccc cgcaacctgg cgaaatcggt gacggtggaa taggcgccaa cggccgcaac
     8821 gcgtttcccg ttccatgaga tttttccgat ccctgcgatg gaatatcgcg atcttcacgc
     8881 ttctggccgc gctttcggcg gcgggtacgt tcctgccgca gaaacacgat tcgcccgaac
     8941 gagtgcgatt ttttttgagc gtccaccccg ccgtgggtcc gttcctggac cggctgggct
     9001 ttttcgatct ttaccattcg gccgggtatg tcgggctgct cgggttgctg gccctcaacg
     9061 tgctggtttg caaacttcgc ggagcgcgga acctcctgtc gtctccaatc cctccgacct
     9121 cggcccctca gacggttgtc gatgtcgagt cgccgctgcc gctcctttcc gcgctcaaca
     9181 gggcaagagg agcgttgtcg gcccgtcact atcgacttcg cgaagagagg gccgaggggg
     9241 agacgcgctt gtgggcgttc cggggtttgg ttcagcggtg gggagacgcc ctgttgcatc
     9301 tcagcgttct gctgatcttg gcgggcgcgg cctatggcgc tgcgatcggg gc
//
LOCUS       DAOTFD010000082         9345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5222_length_9345_cov_4.142842, whole genome shotgun sequence.
ACCESSION   DAOTFD010000082 DAOTFD010000000
VERSION     DAOTFD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_9345_cov_4.142842"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>9345
                     /locus_tag="P5079_06270"
     CDS             <1..>9345
                     /locus_tag="P5079_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29629.1"
                     /translation="LWVKHDLTLEKIAQSVSRTVSFQALLEVAARDMASAVELSRTGR
                     FFEMWDDVRHARSFLMAAQNPDKLTILPDGRIAATAAWQTVLSDFCDEAGHLRFGEFP
                     VDSPQWVVLHYLLGLDLEMARLAASGDIVSALLASPEKRTRFENVLDVAGILKEHNSE
                     IPMGLVRQLSRFLEDDVAYKNLELFSTVKQTFPFIIDVFNADGVAKEVYRDLLKEYLR
                     ADGSLDMGKVFAVEGGLIMFLSKVAVGYFSDDLAAILSRTPFFEKHRAEALKNPAEYL
                     EKLMLLGRIARGNLELGLMEAKYFDEFDKLGEKLGMLVGLSNLMHGIVGGNLTVVERF
                     REIFSAFADSRGSLDVGKIISSEQHFGAFAELIHMSGKNHAVAFYVSRHPVFQKVMSQ
                     KTYEDNLDEIEMELAEVLDGEGKVVKYTEALRGFTDPSTGKLLLEDLLSDADATAAFK
                     ILVGVAARNMAVARWLLQTGWLEKQKRTKDALQNLEKISEVLPDVVDDRGRLKEQYAQ
                     PLKRFAPGAWWPWRRDRTLDLRRVLTDANRAELFVVLARLSNLEPDSASYIARKAGRF
                     LRKFPQLFPKLAGMLGVYRIGGMAPSDLPFVAQMDTALSRSIVSLEKLGRIMPAYASS
                     LKREVAGVVQGDTQKADNFNLIWDILPQVVDEKGNVTAELQVVFKEFADPSGRLLMEK
                     VIGRRLDTLKFMTYYKVALVDLAGAEKLAKGRLIDRYVDPSQLDIDWRWKDMREATVG
                     GLSALAEASPALLDDLLTTGKIKNLVGILGRSERLSLLAGWLRGTRGILEKDGAVSPM
                     YRDIFKDFTDAQGRLNLNDLVTHPAKVYAMIALINLLKLPVEHGPRKGPRFLALATWL
                     VAQPLFQEVLKDEDRAVNLLVLCRSDAFPQVVDGEGKIKREYRRALKKWVVYTDGGFN
                     FSRVLDSLAETRVAAGMMDIAQDITGLAEQISADLAFDKLAGNLEWAKKFEDVAGFLP
                     YFFREDGVLRRYPEVFRGYLDFDSKLDLSRVFKDTVSFQTFYTLCICSMWNVDFVHYL
                     RSTGWLEKMSKDAAVSEVLMHFWKLKRRVGADGTVRREWRGVLAPFLDAQGRVDVSRL
                     AGDQKEQYYLMALIFLAEADKDLAEEAEAAGILRDVFTDWFVEHTEKMGLETEPEIIE
                     EKGETFVGLVALLAKVYGSNPAFARMILSNAEMLEGFLKKEDLLADLSVMQQVFERVL
                     DGEGRVKKAYRKGLSVLCDEDGRVSFPDEVSVAHYFVALAEGITVNRAFVERLADDGF
                     AQSIMRDVRMSKRLREVFPALLEIVDETGALRPEMAGVFGGFLTDGRPDLGKVVGSEA
                     RVSAFKTMVNVVKDNPALARRLAAGDFLGDVLDQHSSRLRPLLSVSSVLGKVCDEAGR
                     VTVEGLGFFCDQTGKINWDKVLSSDKTARLFVALAEIALDEKKAGVSVLGLLEGVLRW
                     DTVLESEKALMGFCREVAPNLLDWYKDNPEILGAIPTLVENGLEYSMLTDSYDFVPLD
                     RELAERLFDHLRDIGRAPPQRRGFQVAYTHMISAASNKSISREQSRPLLSRLLQSKTE
                     QEYYDFLRDELVPRIRENLARERPGSPFLNADWKRLLVLGAVGWDIPGGRRYTMDLDK
                     VVSRVEFVLDHLGVNRDDTAEYLLGLVYALNLRFYTPATIQMFPPGFHRLHEFGMYPW
                     NTGAYWGRFIVGNLLALPSEIAHATGYELDRDLEMIKEAEIQDRLGAVNILRKHSHLA
                     FENASEYVVQATRFSQEILLKWAEGALPPAAEESLLKALKGLVTLERYTQIVSAQKNL
                     LSSEIDRNVLLAMLSPSEVYSLGKLLAKDELSLEDAVVEFRRQFIVHVREKAEIEIDQ
                     AVGVMAMSSEKVRGVKDVVMKPYEFYDESHEVAERITQDVFVKLVLTLSQANIDINIL
                     PYMNAKAMEWVLSKTFQRTDTDWRAVVVQLEQMDVKMVRQWLAELVEEGIVTEDESAS
                     KRVASFEELVLLAVRNGVSLSSALWDAFKKLPPGDLVVGLMDLAAAPAAVASTVTPPT
                     GGAKSAWNAAGMFGLGVVTGTLACAATLLLFGNPLGALLSVLGLHHLVQSVLILMATG
                     RLLPERELVAEAEQLADLTEPPSLKNGFRVAQTDPDGTIRIYKPVFKRLPAVVQSAFI
                     RHERKHGQVRAWSQRFGIFKNAFVEEMAAGVLDNLPSLGGIRSKIKSRSAVFFKRFQK
                     PAFREASTLERDKVFDPLIKVATKTDKVRLIEPNSEEFQRLDPGGVYLTKGANGESTK
                     VYEVTIPSFKMREQAKDNGLSPPWEIVCFADPRHNAVYYFTHRQNYLEMLSDEEHAVV
                     ASAIAKRSRAQIDKVRDLDSVAPVPQISDLRLSYLRFKRALGGPFRGVYAIFDAARQM
                     IRGDVEFKPFLRKERKDFILSMVWFAVSAALAITVPWLIGQMLDVSSLHEVGKAVPSL
                     VPVKVGGVSMGASTGLLILTFGLFLANALDTWQERVSTWVSGRMEARILRNFVNHFVD
                     QISGKSMGFFRKRKSVEIAARIRDMSHIIFKNVHIKLYLPNNIIMLVSSTAMLFLMDF
                     KIAVIVLFTMTTLALLSTLFGKRLEDLDEEVTDSETGLVALASDLFSNMETMKIFNIR
                     PQLMAHFAKRNEALTKVRLKKERTWSYYSAFSTTFSQALTNVSIILIGCWSLFFYGHP
                     SVGEIVAMEAYALSVNSAVEAFSSNWVAFREARGKTKRIMNMLNAVPEIQDNPDAVEV
                     GELQGHVEFRNVNFSYVSGNKVLKNVSFEVKPGQKVAFVGGTGSGKTTVMRLLCRLYD
                     PSAGDILVDGHNLKDVKFTSLADQMAIVPQNSALFDGTVRQNLLFIKPDASEAEMADA
                     LRRAKAEFLFDEEMFPDGLDTEIGEGGSRFSGGQKQRIAIARAVLRNPKVLLLDEATS
                     ALDNESERLVQEGLNDLMKGRTTFVVAHRLTTIMDADVIHFLEKGRIVESGTHAELMA
                     LRGRYYQMWMAGNKAPVKQSEGASKKVGGAVVPATEQEALEEEAEESERLDGATEHTE
                     EAFGIEGEEEDEETEESDDRAKWPVVAWASALVGGAVGVLAFSAWGPLGLLLAAPFSV
                     GPVIQRVFAMANWTRNWNGLGLSARLSAVEELQGWAGVGNVAVEGKPLWNGART"
BASE COUNT         1833 a         2531 c         3030 g         1951 t
ORIGIN      
        1 agctgtgggt caaacacgac ctcaccctgg agaagatcgc ccaaagcgtt tcacgaacgg
       61 tttctttcca ggcccttctg gaggtcgccg cccgcgacat ggcatccgcg gtggagcttt
      121 ccagaacagg ccgctttttc gaaatgtggg acgacgtgcg ccatgcgagg agctttttga
      181 tggcggcgca aaacccggac aagttgacga tccttcccga tggtcgcatt gcggccacgg
      241 ccgcgtggca gaccgttctg tcggatttct gcgatgaggc ggggcatttg cggttcggcg
      301 aattccctgt ggactcgccg caatgggtgg ttttgcacta cctgctcggg ttggatctgg
      361 aaatggcccg gctcgccgcg tccggggata tcgtctccgc cctgttggct tctcccgaaa
      421 agaggacgcg ctttgagaac gtcttggacg tggccggcat cctcaaggaa cacaactcgg
      481 aaatccccat gggattggtg aggcagttaa gtcggtttct ggaggacgat gtcgcctata
      541 agaacttgga actgttttcc acggtgaagc agacatttcc gttcatcatc gacgttttta
      601 acgccgacgg cgtggcgaaa gaagtatacc gggacctttt gaaggagtat ttgagggcgg
      661 acggttcgct ggacatggga aaggttttcg ccgtggaggg cggcttgatt atgttcctgt
      721 ccaaggtggc ggtgggatac ttcagcgacg atctggcggc gatcctgagc cggaccccgt
      781 tttttgagaa gcatcgtgcc gaggccctga aaaaccccgc ggaatacttg gaaaaactga
      841 tgctgttggg acggatcgcc cgcggcaatc tggagttggg gctgatggaa gccaaatatt
      901 tcgacgagtt cgacaaactg ggcgaaaagc tggggatgtt ggtggggttg tccaacctga
      961 tgcacggcat cgtgggggga aacctcaccg tcgttgaaag atttcgggag atattttcgg
     1021 cttttgcgga ttcccggggg tcgctggacg tcggcaaaat catttccagc gagcagcatt
     1081 tcggggcgtt cgccgaactc atccacatga gcggcaaaaa ccacgccgtc gccttctatg
     1141 tctcccgaca cccggtgttt cagaaggtca tgtcgcaaaa aacgtatgaa gacaatttgg
     1201 acgagatcga aatggaactg gcggaggtgc tggacggcga aggaaaggtc gttaaataca
     1261 cggaggcgtt gcggggtttc accgacccgt ccaccggcaa actcttgctg gaggacctcc
     1321 tgtcggacgc ggatgccacg gcggcgttta aaatcctggt gggggtcgcc gcccgcaata
     1381 tggccgttgc ccgctggctt ttgcagaccg gttggctgga aaaacaaaaa cggaccaagg
     1441 acgccctgca gaatttggaa aagatctcgg aggtcttgcc cgatgtggtg gatgatcggg
     1501 gccgcctgaa agaacaatat gcccagccct tgaaaaggtt tgctccgggg gcgtggtggc
     1561 cgtggcggcg ggatcggacg ctcgatctcc gccgggtgtt gacggacgcg aatagggcgg
     1621 agttgttcgt ggtgctggcc cgcctctcca atctcgaacc ggactcggcc tcttacatcg
     1681 cccgcaaggc cggacgtttc ctgaggaaat tcccgcagct tttcccgaaa ctggccggca
     1741 tgctgggcgt ctatcgcatc ggcgggatgg ctccgtccga cctccctttc gtggcgcaga
     1801 tggacacggc tctctcgcgt tccatcgtca gcctggaaaa gttgggaagg atcatgccgg
     1861 cttacgcctc gtccctgaag agggaggtcg cgggagtcgt tcagggagac acccaaaaag
     1921 ccgacaactt caatctgatt tgggacattt tgccacaggt ggtcgatgaa aaggggaacg
     1981 tgaccgccga gttgcaggtc gtttttaagg agttcgccga tccttcgggg cgtttgttga
     2041 tggaaaaagt gatcggccgc cggctggaca ccctcaaatt catgacctat tacaaggtcg
     2101 cgttggtgga cctcgccggc gccgaaaaat tggcaaaagg acgcctgatc gatcgttacg
     2161 tggatccgag tcagttggac atcgactggc gctggaagga tatgcgcgag gcgacggttg
     2221 ggggattgtc cgccttggcc gaggcgagcc cggcgctttt ggatgatctg ttgacgacgg
     2281 ggaagatcaa gaacctcgtc ggaatcctgg gccggtcgga acgactctct ctcttggcgg
     2341 gctggctccg gggaactcgc ggaatcctag aaaaggacgg cgccgtcagc ccgatgtacc
     2401 gggatatctt taaggatttc accgacgccc aggggcgttt aaacctgaac gatcttgtga
     2461 cccaccccgc caaggtctat gccatgatcg cgctcatcaa cctgttgaag cttcccgtcg
     2521 aacatggccc ccgcaagggt ccgaggttcc tcgctttggc gacctggctg gtggctcagc
     2581 cgctcttcca ggaagtcctg aaagacgagg accgggcggt gaacctgctg gtgttgtgcc
     2641 gttccgacgc ttttccccag gtggtggacg gcgaagggaa gatcaaaagg gaataccggc
     2701 gcgccctgaa aaaatgggtg gtgtataccg acggaggttt taatttttcc agggtattgg
     2761 acagtctggc cgagacccgg gtcgccgccg gcatgatgga tatcgctcag gacatcacgg
     2821 ggctggcgga gcagatttcg gccgatctgg ccttcgacaa actggccggc aatttggaat
     2881 gggcgaagaa attcgaggac gtcgccgggt tcctccctta ctttttccgt gaggacggcg
     2941 tcctgcgccg ttatccggaa gtgttcaggg gttatctgga tttcgacagc aaactcgatc
     3001 tctcgcgggt ttttaaggac acggtctctt ttcagacgtt ctataccctt tgcatctgtt
     3061 ccatgtggaa cgtggatttc gtccattatc tgcgctccac cggctggttg gaaaaaatga
     3121 gcaaggatgc cgcggtgtcg gaagtgctga tgcatttttg gaagttgaaa aggagagtgg
     3181 gggcggatgg aacggtgcgc cgggagtggc ggggcgtcct ggcccctttc ctcgatgctc
     3241 aggggcgggt ggatgtgtcg cggctcgcgg gggatcagaa agaacaatac tatctgatgg
     3301 cgctgatctt cctggccgag gccgacaagg acttggccga ggaggcggag gccgccggga
     3361 tcctgcggga cgtgttcacg gactggttcg tcgagcatac ggaaaaaatg ggtttggaaa
     3421 cggagccgga aatcatcgag gaaaagggcg agacgttcgt cgggctggtc gccctgttgg
     3481 cgaaagtgta cggcagcaat ccggcgttcg cgaggatgat tttgtccaac gccgagatgc
     3541 tggagggttt cctcaaaaaa gaggatcttc tggccgattt gtcggtcatg cagcaggttt
     3601 ttgaaagggt cctggatggc gagggccggg ttaaaaaggc gtaccggaaa gggctctccg
     3661 tcctctgtga cgaggacggc cgggtgagtt tcccggacga agtctccgtg gctcattatt
     3721 ttgtcgcgtt ggccgagggg atcaccgtca accgcgcctt cgtggaacgt ctggcggacg
     3781 acggattcgc tcagtcgatc atgcgggacg tccgtatgtc caaacggttg cgcgaggtct
     3841 ttccggccct gttggagatt gtggacgaga cgggcgcgct ccgtccggag atggccggag
     3901 ttttcggcgg ttttctgacg gacggtcgac cggatctggg aaaggtggtc ggttcggagg
     3961 cgcgggtgtc ggcttttaag accatggtca atgtcgtcaa agacaaccct gcgttggcgc
     4021 ggcgcttggc cgccggcgac tttttggggg acgttttgga ccagcattcg tcccgcctgc
     4081 ggccgctctt gagcgtttct tccgttttgg ggaaagtatg cgacgaggcc ggccgggtca
     4141 cggtggaggg gctggggttt ttctgcgatc aaacagggaa gatcaattgg gacaaggtct
     4201 tgtcctcgga caagacggcc cggctgttcg ttgcgttggc ggagatcgca ctcgacgaaa
     4261 agaaggcggg ggtatccgtc ttggggcttc tggagggggt ccttcgctgg gacacggttt
     4321 tggaatccga aaaggccttg atgggatttt gccgcgaagt ggcgcccaac ctcctggatt
     4381 ggtataagga caaccccgaa atcctcggcg ccatccccac cctggtggag aacggcctcg
     4441 aatattccat gctgaccgac agttatgatt tcgtgccgct cgaccgtgaa ttggcggaga
     4501 ggctgtttga tcatttgagg gacatcggcc gagccccgcc tcagcgccga ggtttccagg
     4561 tcgcctacac ccacatgatt tccgccgcca gcaacaagtc catctccaga gaacagtccc
     4621 gtccgttgtt gtcccgcctg ttgcaatcca aaaccgaaca ggaatactat gattttctcc
     4681 gggacgaact tgtcccccgc attcgtgaga acctggcgcg ggagcgtcca gggtctcctt
     4741 ttctgaacgc ggactggaaa cgtcttttgg tcctgggcgc cgtgggctgg gacattcccg
     4801 gcggaaggcg atacaccatg gatctggaca aggttgtttc ccgcgtcgaa ttcgttctgg
     4861 accatttggg cgtcaaccgc gacgacaccg cggaatatct cctcggactg gtctacgccc
     4921 tgaacctccg tttttatacc cccgccacca tccaaatgtt ccctccgggg tttcaccgcc
     4981 tccatgagtt cggcatgtat ccctggaaca ccggcgccta ttggggacgg ttcatcgtcg
     5041 ggaatttact ggcgctcccg tcggaaatcg cccacgccac cggttatgaa ctggaccggg
     5101 acctggagat gatcaaggag gcggagattc aggaccgcct gggcgcggtg aacatcctcc
     5161 ggaaacattc gcatctggct tttgagaacg cctcggaata cgtcgtgcag gccacgcgtt
     5221 tcagccagga gattcttctg aaatgggcgg agggggcgct gccgcctgcc gcggaggagt
     5281 ctttgttgaa ggcgttgaag gggctggtca cgctggaacg atacacgcag atcgtgagcg
     5341 cccagaaaaa ccttctgagt tcggagatcg acaggaacgt cctcctggcg atgttgtcgc
     5401 cttccgaagt atacagtttg ggaaaactgc tggcgaaaga cgaattgtcc ctggaagacg
     5461 ccgtcgtgga attccgccgg caattcatcg tccatgtcag ggagaaggcg gaaatcgaga
     5521 tcgaccaggc ggtgggcgtg atggcgatgt cttccgaaaa ggtgcgcggc gtcaaggacg
     5581 tcgtgatgaa accgtacgaa ttttacgacg agtcccatga ggtggcggag cggatcaccc
     5641 aggacgtgtt cgtgaagctg gtcttgacgt tgagccaggc caacatcgac atcaacatcc
     5701 tcccctatat gaacgccaag gccatggaat gggtcttgtc caagacgttc cagcggaccg
     5761 atacggattg gcgggccgtg gtggtgcagt tggagcagat ggacgtcaaa atggttcgcc
     5821 aatggctcgc cgaattggtt gaggagggca tcgttaccga ggacgaatcc gcgtcgaaac
     5881 gggtcgcctc cttcgaggag ttggttctgc tggcggtgcg gaacggcgtt tctctctcgt
     5941 cggcgctgtg ggacgctttc aagaaactgc cgcccggcga tctggtggtc ggcctgatgg
     6001 atttggcggc ggcccccgcc gctgtggcgt cgacggtgac gccgccgacg ggcggcgcca
     6061 agtccgcctg gaacgccgcc ggcatgttcg ggctgggcgt ggtgaccgga acgttggcgt
     6121 gcgcggcgac gctccttctg ttcgggaatc ccctgggggc gctcctgtcc gttttggggt
     6181 tgcaccatct ggtccaatcc gtcctgatcc tcatggccac gggccggctc ctccccgaaa
     6241 gggaactggt ggccgaagcc gaacaactcg ccgatttgac cgaaccgccc tccctgaaga
     6301 acggattccg cgtcgcccag accgaccccg acggaaccat ccgaatttac aagcccgttt
     6361 ttaaacggtt gcccgccgtt gtgcagagcg cgttcattcg gcacgagcga aaacatgggc
     6421 aggtccgcgc ctggtcgcag cgtttcggca ttttcaaaaa cgcgttcgtc gaggaaatgg
     6481 cggcgggcgt tctggacaac ctgccgtcgc tgggcggcat acgatctaaa ataaaatcgc
     6541 ggtcggcggt gttttttaaa cggttccaaa aaccggcttt ccgggaagcc tccaccctcg
     6601 aaagagacaa ggtgttcgat ccgctcatca aggtcgcgac caagacggat aaggtccggc
     6661 tgatcgaacc gaattcggag gaattccagc ggctcgatcc gggaggcgtc tatctgacga
     6721 agggcgccaa cggcgaatcg accaaggtct atgaagtgac catccccagt ttcaagatga
     6781 gagagcaggc caaagacaac ggtctttcgc ccccgtggga gatcgtgtgt tttgccgacc
     6841 cccgccacaa cgccgtctat tatttcacgc accgacagaa ttatctggag atgctttccg
     6901 acgaggaaca tgccgtcgtc gcctcggcca tcgccaaacg gtcgcgggcc cagatcgaca
     6961 aggtgcgcga cttggacagc gttgcgcccg tcccccaaat cagcgacttg aggctttcct
     7021 acctccggtt taagcgggcg ttgggaggcc cgttccgcgg cgtgtatgcc atcttcgacg
     7081 cggcccgtca gatgatccgg ggcgacgtcg agtttaaacc gttcctgagg aaggaaagga
     7141 aagacttcat cctttcgatg gtctggttcg ccgtttccgc cgccttggcg atcaccgttc
     7201 cgtggctgat cgggcagatg ctcgacgtga gcagtcttca tgaggtggga aaggcggtcc
     7261 cttcccttgt tcctgtcaag gtggggggcg tttccatggg ggcgtcgacg gggcttctga
     7321 ttctgacttt cgggcttttc ctggcgaacg cgttggatac gtggcaggag cgggtcagca
     7381 cctgggtgag cggccgcatg gaggcgcgga tcctgcgcaa tttcgtcaac catttcgtcg
     7441 accagatttc ggggaaatcg atgggcttct tccggaagcg gaaatccgtt gagatcgcgg
     7501 cccggatacg cgacatgtcg cacatcatct tcaagaacgt gcacataaag ctgtatctgc
     7561 cgaacaacat catcatgctg gtctccagca cggccatgct gttcctgatg gatttcaaga
     7621 tcgccgtcat cgtgttgttc accatgacga cgctggcgct cctctccacg ttgttcggca
     7681 aaaggctgga agacctcgac gaggaggtga ccgattccga gaccggtctg gtcgccctgg
     7741 ccagcgacct gttttcgaac atggagacga tgaagatctt caacatccgg ccgcagctca
     7801 tggcccattt cgccaaacgg aacgaggcgt tgaccaaggt gcggttgaaa aaagaacgca
     7861 cctggtccta ttacagcgcc ttctccacca ctttttccca ggcgttgacg aacgtcagca
     7921 tcatcctcat cgggtgctgg agcctgttct tctacggcca ccccagcgtt ggagagatcg
     7981 tcgcgatgga ggcttacgcg ctgtcggtga actccgccgt ggaggcgttc agctccaact
     8041 gggtggcgtt ccgcgaggcg cgcggaaaga ccaagcgcat catgaacatg ctgaacgccg
     8101 tgccggagat tcaggacaat cccgatgccg tcgaggtggg ggagttgcag gggcatgtgg
     8161 agttccgcaa cgtcaatttc tcctacgtca gcggcaacaa ggtcctgaaa aacgtctctt
     8221 tcgaggtcaa gccgggacag aaggtggcgt tcgtcggcgg caccgggtcc ggaaagacca
     8281 ccgtcatgcg cctcctctgt cggttgtacg accccagcgc cggcgacatc ctcgtggacg
     8341 gtcacaacct caaggacgtt aagttcacca gcctggccga ccagatggcc atcgtgccgc
     8401 agaacagcgc cctgttcgac ggcacggtga ggcagaacct cctcttcatc aagccggacg
     8461 cgagcgaggc ggaaatggcg gacgccctgc gccgcgccaa ggcggagttt ttgttcgacg
     8521 aggaaatgtt tccagacgga ctcgacacgg agatcggcga ggggggctcg cgcttctccg
     8581 gcggacagaa acagcggatc gccatcgccc gggctgtcct gcgcaatccc aaggtgctgc
     8641 tgttggatga ggccacctcc gcgctggaca acgagtcgga acggctcgtc caggaaggat
     8701 tgaacgattt gatgaaaggc cggaccacct tcgtggtggc gcaccggctc accaccatca
     8761 tggacgcgga cgtcatccat ttcctcgaaa aaggccggat cgtcgagtcg gggacacacg
     8821 ccgaactcat ggcgttgcgc ggccggtatt accagatgtg gatggccggc aacaaggctc
     8881 ccgtgaagca gtctgagggc gcctcgaaga aggtcggggg ggccgtcgtt cctgccacgg
     8941 agcaagaggc gctcgaggag gaggccgagg agtcggagag gctggacggg gccaccgaac
     9001 atacggaaga ggctttcggt atcgaaggag aggaggagga cgaggagacc gaggagtcgg
     9061 acgaccgcgc caagtggccg gtggtcgcct gggcgtcggc gttggtcggc ggggcggtcg
     9121 gcgtgctggc gttctcggcg tggggaccgc tgggacttct gttggccgcg ccgttttccg
     9181 tcgggccggt gattcagcgg gtttttgcga tggcgaactg gacgcggaat tggaacgggc
     9241 ttggactttc ggcgcgtttg tcggccgtgg aggagttgca gggctgggcc ggcgtcggga
     9301 acgtggcggt ggagggaaaa ccgctctgga acggcgcccg gacct
//
LOCUS       DAOTFD010000083         9310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5248_length_9310_cov_4.371367, whole genome shotgun sequence.
ACCESSION   DAOTFD010000083 DAOTFD010000000
VERSION     DAOTFD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_9310_cov_4.371367"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="P5079_06275"
     CDS             complement(<1..249)
                     /locus_tag="P5079_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29630.1"
                     /translation="MLKKRILVVDDDKDIRRLVENILMKDGFVTVGAESAADAFKKIQ
                     ASKPDLIILDLQLPDKDGFEVCKTLRGDSSTKYIPVVIL"
     gene            complement(335..1819)
                     /locus_tag="P5079_06280"
     CDS             complement(335..1819)
                     /locus_tag="P5079_06280"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY29631.1"
                     /translation="MKVTLRQKFTLFASLLVLVVASGVGVSLYLAERLYLVQRIQQSQ
                     IEMVQVLSQIGREALANNSELLLSSYLALVRRSRALSYAMVLAADGRILAHSNVILVG
                     QKPTDPITRKALETQSLLRQVMDVSGDQLIDLSLPIYVSGRRGATARVGYSQSATRQL
                     VDESLAAARRRIAFATAIALLFGLGGALLLAYYIARPIQHLRDGVREIGEGALHHRLE
                     VTSRDELGDLAEEVNIMAQKLEELDQMKQDFVSNVTHELRSPLTSLRGYVEFLLRGDA
                     GALNEEQTEQLIVVKNNAARLAKFIDNLLDVAKIEASKTELHPESVSLQKLCREMLVL
                     FRPQAHEKNIEFAGAVPENVSPVWADADKTSEILINLLSNAFKFTPPQGRVTLSASDE
                     GELVHVSVRDTGIGIPAASLQSVFNKFEQVKPTEGLVRKTKGTGLGLTIVKGFVEAQQ
                     GKVWLESQLNVGTTVHFTLPKFKPDAGHAFDDGESEEADLARPV"
     gene            2129..>9310
                     /gene="lon"
                     /locus_tag="P5079_06285"
     CDS             2129..>9310
                     /gene="lon"
                     /locus_tag="P5079_06285"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY29632.1"
                     /translation="MAIFGIAWWNLRRHSFWKKSFAAALTAVFVFTVVSTPLAEANFW
                     QERQRAHTDRRQEKQASLLASRSDSRLSNLPLAGLDLPEKSGAITETYWPPVSLKSDL
                     AQPLVIHLQDAHGVFAAQKNAADIVSHFLHDTPPVTLVAVEGAWGELNTRWFSSFPQE
                     DLRDSLAAVLLREGDISGEEYLAVTQPANGLSLFGVEDAELYFANNAAREETREDRRR
                     VLAYLRGLTARLDQLKPRVFNRTLLELDRLSAAHGTQTLPLKEYAQGLRRFWGDELPA
                     AYPNARKILELVSLEKNLDSARIEGERRRLVETLGRTAPEDTLSALLSAAMDYRMGRL
                     SPRQYHRMLLSAAQTAGVPCPELDRFVRYLEKYETLDLGLLSAELENLTEALGARLAT
                     RPLEKRLIQADRWIALKEKLWSLEMSPQEWAVYPQRNTFDSWQTVETHLAEMEAAAGV
                     TVAASDEWVSLRPVIRRAEPLAERFFELALRRNNALVQNTLAEARRRKASRVILIAGG
                     FHTPGLARLLRQEGASYVVVRPRLDRDNAPFERAERRDTLHSFLQAVKTAHGALRTAS
                     RFQDLFGRQTLAAELLGLTALSLSPEETEKLADDWLSRLSSADDDTVANVAIFPRTFI
                     RRGQTYVLGAIQGRKARLPFVLWNEGTAQEPRLRALHADADIRRWMAEGPAEVSDLAV
                     EERRGLGVRLKRMWPHLLEKDDALPAFLGLPPDPTAKVETSASSARSADLPAPSAPRS
                     PRLSLGRALVIGGVVLAGAVIVSYLLFPGFFQNLLHALAAKSPVSLQLAGLFGTVIVT
                     AGGQTVDTYKTAYASDKDTQKQVLSQLIAEKTSAAHAAAEEIIQLLQAMDDKRAAELY
                     GELAPNQKKALLIAFDAHTIGRLLNGRAVLARTERLDALRTRRAELSVTLADMKRILA
                     DLKTGKRPSQELLKKTLAPQIEELEDDIAEHEDLLAEYSTLIDGNTIPRIGNNPPSLK
                     NQAETDKQNFQAELQDMKRRLQSLQRGEVSVDQIRKQMSDLEKHLALINDEINVFRYP
                     AISLTPAQNSERVEALIAAKIQEEVEFIQKTLYARTDGETKLNRIWDVFKGAQAVTES
                     IEFESGLVDLSAKAARDIADGVPAPAGLDKLPEYNSVLPHIYRSLRQRTNPAIAVSIP
                     TDKAKAPLNYLMARRLLEDARYRPTHKRQKILSLSFKNLPQGGEQAVERIKELLRAAH
                     QMGDTAIVLDLDIFPAFFLQRDVEALDNILGYWQGLPNPPPLVAFGSPNTQEVYLNHS
                     QFYPLVFSERQDLALSADTQRTLLGMEAAEIEKFNDIRIPAEIVSTLLKRVLSSGPFN
                     LAMAIRVLQEAASEAEARGRDELSENEVLKAVADLPAAENLPLEERFRHLAEQMPERA
                     RQIARQQLERLKRLPPNSPERGVITNYLENLVQIPWTKRSAEETDLGKVRDVLERSHF
                     GMKEAKEQVLDYIAERTLAAKKQGKIICWVGPPGVGKSTLAETVARATGREFVRVSLG
                     GVRDEAEIRGHRRTYIGAMPGIIVKGLQKSKSRNPVFLLDEVDKMSSDSHGDPVAALM
                     QVLDPKQNHEFQDHYMEVGVDLSEVMFILTANDLNTIPGPLLDRMEIVQLPGYTPNEK
                     IVIGRDYTLPRIAKTLGLTETQVRIPDPTAVVETLVDGYTQEAGARQLENRLRKILVK
                     VLHEWSLNRRSPVVLTPAKVAEYLGAPREGEKIREEDTVGEAVGLAWTPVGGEILYVQ
                     STLLDTGDAGRELSVDITGNLMDTMKESAALALSVARNKMRQWGLNAARFNGKRIFVH
                     VPQGSIPKDGPSAGITFVTSILSELSGRPVRSDVAMTGEVDAKGNVLPIGGLKEKLLA
                     ARKAGVKTVFVPLKNRGMLIDDVFKTVPELRGVLESRGRQTLFVPKMESDGEWKSFLQ
                     ELRRNRPKGLTRRDAEDGITLAGDRNAMREFLKRFPQLAGPLTYVLAEKVEDVLEQAL
                     LPSKSSETSPTPSAGSSRSRGAVSLPVLFVLAGLAALVLLLLPVWPILMDWTQSSLAF
                     AGTLSPAKMSVLTWPLLIGGVAVSNLGNMNAPAGAQPNVPEETKTQQLRSLLDAGTPD
                     GLKTAKTFLQTVWSYSQPLAGKLFDSFANLSRYRDRLLFAFDPQTLGRLLFATRLALR
                     EDYLEQRRSTVLKQKELLEKFAADLSQAESLSPKNRDAFLAARRTELLAFLEEDLKAT
                     ETEAARLRNILKQQKTAGRSPLQAPPGSFSPEEMLKHLNASIENAKKTMSLLKNDPKE
                     ALAGLQEQLKRIEEGLGREEREVLDLEFPQMSRDPEEQANLLDSYLYTRVIERMQEIL
                     KAFDPHKETRAHIQIVWDTYVANFYLHGLENNGLVRDISKSISREKGLTDVDELYRPA
                     TDTVVRILGQRTERALTLVAPSLATKGPLA"
BASE COUNT         1870 a         3126 c         2701 g         1613 t
ORIGIN      
        1 caggatgacg acggggatgt atttggtgga ggaatcgccc cgaagggtct tacagacctc
       61 gaacccgtcc ttgtccggca gctgcaggtc cagaatgatc aggtccggtt tggacgcctg
      121 gatcttcttg aacgcgtccg cggcgctctc cgccccgacg gtcacgaagc cgtccttcat
      181 caagatgttc tccaccagtc ggcggatgtc tttatcgtcg tctactacca ggatgcgctt
      241 cttcagcata ctttctcctc aaaggcctcg gacgtatgac gaatggtagg aaatttttcg
      301 gatgtttgta aaggacgacg cgggaagaaa aagttcaaac cgggcgggcc aggtccgcct
      361 cttcggattc gccgtcatcg aaggcgtgac cggcgtccgg cttgaatttg ggcagggtga
      421 agtgcaccgt cgtgccgacg ttcaactggc tttccagcca gaccttgcct tgttgcgcct
      481 ccacaaaccc cttgacgatg gtgagcccca gaccggtgcc cttggtcttc cggacgagcc
      541 cctcggtggg tttcacctgt tcgaacttgt tgaagacgct ctgcagagag gccgccggga
      601 tgccgatgcc cgtgtcccgc accgacacat ggacgagctc cccttcgtcc gacgcgctca
      661 aggtcacgcg accctggggg ggggtgaatt tgaaggcgtt gctgaggagg tttatcaata
      721 tctccgaggt tttgtcggcg tcggcccaca ccggggaaac gttctccggc acggcgccgg
      781 cgaactcgat gttcttctcg tgcgcctggg gccggaagag caccagcatt tcccggcaca
      841 gtttctgcag ggagaccgat tcgggatgca gctccgtctt gctggcctcg atcttggcca
      901 cgtccagcag gttgtcgatg aacttggcca gccgggcggc gttgttcttg accacgatga
      961 gttgttcggt ctgctcctcg ttgagggcgc cggcgtcgcc gcgcaggagg aactcgacat
     1021 atcccctcag cgaggtcaac ggggaacgga gttcgtgcgt cacgttggag acgaaatcct
     1081 gcttcatctg gtcgagttcc tcgagtttct gggccatgat gttcacctct tcggccagat
     1141 cgcccaactc atcgcgcgag gtgacctcca accggtgatg caacgcgccc tccccgattt
     1201 cccgaacgcc gtcgcggagg tgttgtatcg ggcgggcgat gtaataggcg aggagcaggg
     1261 cgccgcccag gccgaacagc agggcgatgg ccgtggcgaa ggcgatccgc cggcgggcgg
     1321 cggccagcga ttcgtccacc aactggcgcg tggcggactg ggaatacccg acccgcgccg
     1381 tggccccccg gcggcccgac acgtagatgg gaagggaaag atcgatcaat tggtcccccg
     1441 acacgtccat cacctgccgc aggagagact gggtctccag ggctttccgt gtgatgggat
     1501 ccgtcggttt ttggccgacc aggatgacgt tgctgtgcgc cagaatgcgg ccgtcggccg
     1561 cgaggaccat ggcgtaggaa agggcgcggg agcgccgcac caaagcgagg taactcgaaa
     1621 gaagaagttc gctgttgttg gcgagcgcct cgcgcccgat ctgggaaagc acctggacca
     1681 tctcgatctg gctctgttgg atgcgttgca ccagatagag ccgttccgcc aagtagagag
     1741 aaacgccgac gccggaggcg acgacaagga cgagcaggct ggcaaagaga gtgaactttt
     1801 gtcgaagagt aactttcaat gtattttcag tctaccagag ctttttgtca cccgtcaagg
     1861 gggaaattgt cacgcccgtc cggccaaaag atctcccggg cttcaaagga atatgtttac
     1921 atatatgtta caatttgttt attcttctat ataagaattt ggtctatcct tgtggcagcc
     1981 aaaaacccat ggatcccatg aacagaaaac atcgtttctc ccgttccttt ccgttccccg
     2041 gtctttccgg tttcacgtcc gttcgccccg aatgacctcc ttcccgtccc tcttctccat
     2101 gagtctcttt agcaatagcg acaaaaggat ggcaattttt ggaatcgcct ggtggaacct
     2161 ccgccggcat tctttctgga aaaaaagttt cgcggcggcg ttgaccgccg tcttcgtttt
     2221 caccgtggtg tcgacgcctt tggccgaagc caacttttgg caggaacgtc aacgcgccca
     2281 caccgaccgg cggcaggaaa aacaggcgtc tctcctggct tcccggagcg actcgcgtct
     2341 ttcgaacctc cccctggccg ggctggatct tcccgagaaa agcggggcga tcacggaaac
     2401 ctattggccg ccggtcagcc tgaaatccga tctcgcccaa ccgctggtca tccacctgca
     2461 ggacgcccac ggcgttttcg cggcgcagaa aaacgccgcc gacatcgtct cccattttct
     2521 gcacgacacc cctcccgtca ccctggtcgc cgtggaaggc gcctgggggg aactcaacac
     2581 ccgctggttc tcgtctttcc cgcaggaaga cctccgcgat tcactcgccg ccgtccttct
     2641 gcgcgagggg gacatctcgg gggaagaata tctggccgtc acccagccgg cgaacggtct
     2701 ttcgctcttc ggcgtggaag acgcggaact ctattttgca aacaacgccg cccgggagga
     2761 aacgcgggaa gaccgccggc gcgtgctggc gtatctgcgg gggttgacgg cccgcctcga
     2821 ccagctgaaa ccccgcgtgt tcaaccgaac cctgctggaa ttggaccgcc tctccgccgc
     2881 ccacgggacc caaacccttc ccctgaaaga gtacgcgcaa gggctgcgcc ggttctgggg
     2941 ggacgaactg cccgccgcct atcccaacgc ccggaaaata ctggagctgg tctccctgga
     3001 gaaaaacctg gactccgcgc gcatcgaggg cgaacgccgg cggctggtcg aaacgctggg
     3061 ccggaccgcc ccggaagaca cgttgtccgc ccttctctcc gccgccatgg actatcgcat
     3121 gggccgcctc agcccgcggc aataccaccg gatgctgctc tcggccgccc agacggccgg
     3181 ggtcccctgt ccggaacttg atcggttcgt ccgctatctg gagaaatacg agacgctcga
     3241 cctcggcctc ctgagcgccg aactggagaa cctcacggaa gctctcgggg cgcgcctggc
     3301 gacgcggcct ctggagaaaa gactcatcca ggccgaccga tggatcgccc tcaaggaaaa
     3361 gctttggtct ctggagatga gccctcagga atgggcggtc tacccgcagc ggaacacctt
     3421 cgactcctgg cagaccgtgg aaacccatct ggccgaaatg gaagccgcgg cgggggtcac
     3481 cgtcgcggct tcggacgaat gggtctccct gcggcccgtc atccgccggg cggaaccgct
     3541 cgccgaacgg ttcttcgagc tggccctccg gcggaacaac gccctcgtcc aaaacacgct
     3601 ggcggaagcg cgtcgtcgga aagcgtcccg cgtcattctg atcgccgggg gattccacac
     3661 gcccggcctc gcccgtctcc tgcgccagga aggcgcctcc tacgtggtgg ttcgcccgcg
     3721 tctcgaccgg gacaacgccc cgttcgaacg ggccgaacgc cgcgacaccc tgcattcctt
     3781 tctgcaagcg gtgaaaacgg cccacggcgc gctccgtacg gccagccgat tccaggacct
     3841 tttcgggcgg cagaccttgg cggcagaact cctgggtctg accgcgctct ccctttcgcc
     3901 ggaagaaacg gaaaaactgg cggacgactg gctctcccgc ctttcctcgg ccgacgacga
     3961 caccgtggcc aacgtcgcca ttttcccgcg gacgtttatc cgccgcggac aaacctacgt
     4021 gctgggcgcc atccagggaa ggaaagcgcg gcttcccttc gtcctttgga acgaaggcac
     4081 cgcgcaggaa ccccgtcttc gcgccctgca tgccgacgcg gatatccggc gatggatggc
     4141 ggaaggcccg gcggaggtct cggacctcgc cgtggaggaa cgccgggggc tcggcgtccg
     4201 tctcaaacgg atgtggcctc acctgctgga aaaagacgac gcgctccccg ccttcctcgg
     4261 cttgccgccg gacccgaccg caaaagtcga aacctccgcc tcgtccgccc gctcggccga
     4321 tcttcccgcc ccttccgcgc cccggtctcc gcgcctctcc ctgggccggg cgttggtgat
     4381 cggcggagtc gtcctcgcgg gggcggtgat cgtctcctat ctccttttcc ccggtttctt
     4441 ccagaacctt ctccacgcgc tcgccgccaa aagtcccgtc tccctgcaac tggcggggtt
     4501 gttcggcacc gtcatcgtca ccgccggcgg ccagaccgtg gacacttata aaaccgccta
     4561 cgcctcggac aaagacaccc agaaacaggt cctctcgcaa ctgatcgcgg aaaagacctc
     4621 cgccgcccac gcggcggcgg aagaaatcat ccagctcctc caagccatgg acgataaacg
     4681 cgccgccgaa ctctacggcg agttggcgcc gaaccagaaa aaagccctcc tcatcgcctt
     4741 cgacgctcac accatcggcc ggctgctcaa cgggcgggcg gttctggccc gcaccgagcg
     4801 attggacgcc ctccggacac gacgggcgga actctccgtg acgctggcgg atatgaaacg
     4861 gatcctggcc gacctgaaaa ccggcaagcg accctcccag gagctcctca aaaagacgct
     4921 ggccccgcag atcgaggaac tcgaggacga catcgccgaa cacgaggacc tcctcgccga
     4981 atacagcacg ctcatcgacg gcaacaccat cccgcgcatc ggcaacaacc cgccctctct
     5041 caagaaccag gccgaaacgg acaaacagaa ctttcaggcg gaacttcagg acatgaaacg
     5101 gcggctccag agtcttcaac ggggtgaggt ctccgtcgac cagatccgga aacagatgtc
     5161 ggacctggaa aaacacctgg ccctgattaa cgacgagatc aacgttttcc gctaccccgc
     5221 catctccctg acccccgcgc agaacagcga acgggtcgaa gccctgatcg ccgcgaagat
     5281 ccaggaagag gtggagttca tccaaaaaac gctctacgcc cggacggacg gcgagacgaa
     5341 attgaaccgc atctgggacg tcttcaaggg ggcgcaggcc gtcaccgaaa gcatcgagtt
     5401 cgaaagcgga ctggtggacc tgtcggccaa agcggcccgc gacatcgccg acggcgtgcc
     5461 ggcgcccgcc gggctcgaca agctgccgga atacaactcc gtgcttcccc acatttaccg
     5521 ctccctgcgc cagcgcacca accccgccat cgcggtgtcc atcccgacgg acaaagccaa
     5581 agccccgctc aactatctga tggcccggcg gttgctggaa gacgcccgtt accggccgac
     5641 ccacaaacgt caaaagatcc tttctctctc tttcaagaac ctgccgcagg gcggagaaca
     5701 ggccgttgaa cgcatcaaag agcttctgcg ggcggcccat caaatgggcg acaccgccat
     5761 cgtcctggac ctggacatct tccccgcttt cttcctccag cgcgacgtcg aggctctgga
     5821 caacatcctc ggctactggc agggactccc caacccgccg cccctggtgg cgttcggttc
     5881 ccccaacacc caagaggtct atctcaatca ttcccaattc tatcccctgg ttttcagcga
     5941 acggcaggac ctggctctct cggccgacac ccagaggacc ttgctgggga tggaagcggc
     6001 cgagatcgaa aaattcaacg acatccggat tccggcggaa atcgtctcca ccctcctgaa
     6061 acgcgtcctc tcgagcgggc ccttcaatct ggccatggcc atccgggtcc tccaggaagc
     6121 cgcctccgag gccgaagcgc gcggccgcga cgaactttcc gagaacgagg tgctcaaggc
     6181 cgtcgccgat ctccccgccg ccgaaaacct gccgttggag gaacgtttcc gccacctggc
     6241 cgagcagatg ccggaacggg cgcgccagat cgcccgccaa caactcgagc gcctcaaacg
     6301 tctgccgccc aacagtccgg aacggggagt catcaccaac tatctggaaa acctcgtgca
     6361 gatcccctgg acgaagcgat cggccgagga aaccgacctg gggaaggtcc gagacgtcct
     6421 ggaacgttcc catttcggca tgaaagaagc gaaggaacag gtgctggatt acatcgccga
     6481 gcggaccctg gccgccaaga agcaggggaa aatcatctgc tgggtcggcc cgccgggcgt
     6541 cggcaagagc accctggccg agacggtggc gcgggccacg ggccgggagt tcgttcgcgt
     6601 cagcttgggc ggcgttcgag acgaagcgga aatccgcgga caccggcgca cctacatcgg
     6661 cgccatgccg ggaatcatcg tcaaaggcct tcagaaatcc aagtcgcgca accccgtctt
     6721 cctcctggac gaggtggaca agatgtcgtc cgattcccac ggcgatccgg tggcggcgct
     6781 gatgcaggtc ctcgatccga aacagaacca tgaattccag gaccattaca tggaggtcgg
     6841 cgtggacctt tcggaggtca tgttcatcct caccgcgaac gacctcaaca ccattcccgg
     6901 cccgctgctg gaccgcatgg agatcgtgca actccccggc tacaccccca acgagaagat
     6961 cgtcatcggc cgggactata ccctgccccg gatcgcgaag accctcggcc tcacggagac
     7021 gcaggtgcgg atccccgacc cgacggccgt ggtggaaacc ctggtggacg gatacaccca
     7081 ggaggcgggc gcccggcaac tggaaaaccg cctgcggaaa attctcgtca aggtcttgca
     7141 cgaatggagc ctgaaccgac gttcccccgt cgtcctcacg ccggcgaaag ttgcggaata
     7201 cctcggcgcg ccgagggagg gggagaaaat ccgcgaggag gacaccgtcg gcgaagccgt
     7261 ggggttggcc tggaccccgg tcggcggcga aatactctac gttcaatcca cccttctcga
     7321 caccggcgac gccggcaggg aactttcggt ggacatcacc ggcaacctga tggacaccat
     7381 gaaggagagc gcggcgctgg cgctcagcgt ggcgcggaac aaaatgcgcc aatgggggct
     7441 gaacgccgcc cggttcaacg gaaaacggat tttcgtccac gtcccccagg gatccatccc
     7501 caaggatgga ccgtcggcgg ggatcacctt cgtcacctcc atcctctcgg aactttccgg
     7561 ccgcccggtc cgaagcgacg tggccatgac cggcgaggtg gacgccaaag gcaacgtgtt
     7621 gcccatcggc ggattgaagg agaaactcct ggccgcacga aaagccggcg tcaagaccgt
     7681 gttcgtgcca ctgaagaacc ggggcatgct catcgatgac gtcttcaaaa ccgtgccgga
     7741 actccgcggc gtcctggaat cccgcggacg gcagaccctc ttcgtcccca aaatggagtc
     7801 cgacggcgaa tggaaatctt tcctccagga actgaggcgc aaccgcccca aagggttgac
     7861 gcgccgcgac gccgaggacg gcatcaccct cgccggagac cgcaacgcca tgcgggaatt
     7921 cctgaaacgg ttccctcagc tggccggccc cctcacctac gtcctggcgg aaaaagtgga
     7981 ggacgtcctc gagcaggccc tgctcccctc gaaatcgtcg gaaacctcgc ccacgccttc
     8041 ggcggggtcc tcccgttccc gcggagccgt ctccctcccc gtcctcttcg tcctggcggg
     8101 cctggccgcc ctggttctcc tgctcctccc ggtttggccg atcctcatgg actggacgca
     8161 atcctccctc gccttcgccg gaaccctttc gccggcgaag atgagcgttc tgacgtggcc
     8221 cctcctgatc ggcggggtgg ccgtttcgaa tctggggaac atgaacgccc ccgccggcgc
     8281 gcagccgaac gtcccggaag aaacgaaaac acaacagctc cgctccctct tggacgccgg
     8341 cacccccgac ggcttaaaaa cggcgaaaac gttcctccag accgtgtgga gttacagcca
     8401 gccgctggcg gggaaactgt tcgattcctt cgccaacctc tcgcgctacc gggaccgcct
     8461 gcttttcgcc tttgatcccc agaccctcgg gagactcctc ttcgccaccc gactggccct
     8521 gcgggaggac tatctggaac aacgccgctc caccgtgttg aagcaaaagg aactcctcga
     8581 gaaattcgcc gcggaccttt cccaagcgga atctctgtct cccaagaatc gagacgcctt
     8641 cctcgccgca cgacggacgg aattgctggc gttcctggaa gaagacctca aagcgaccga
     8701 aacggaagcc gcccggcttc gcaatatcct caaacagcaa aagaccgccg gccgttcgcc
     8761 tctccaagcg cctccgggat ctttttcccc cgaagagatg ttgaagcacc tgaacgcctc
     8821 catcgaaaac gccaagaaaa cgatgtctct cctcaagaac gatcccaagg aagccttggc
     8881 ggggctgcaa gagcaactca aacgcatcga agaggggttg ggtcgggagg aacgcgaagt
     8941 gctggacttg gaattccccc agatgagccg agaccccgaa gaacaggcaa acctcctcga
     9001 ttcctatctc tacacgcgcg taatcgaacg catgcaggaa atcctcaagg ccttcgatcc
     9061 gcacaaggaa acccgggccc acatccagat cgtctgggat acgtacgtgg ccaactttta
     9121 tcttcacgga ctggaaaaca acggcctggt gcgggacatc tcgaaatcca tttcacgcga
     9181 gaaaggcctc accgacgtcg acgaactcta ccgtccggcc acggacaccg tggtccggat
     9241 attgggccaa cggaccgaac gcgccctgac gctggtcgcc ccgtccctcg ccaccaaagg
     9301 gcccctcgcg
//
LOCUS       DAOTFD010000084         9283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5266_length_9283_cov_4.345687, whole genome shotgun sequence.
ACCESSION   DAOTFD010000084 DAOTFD010000000
VERSION     DAOTFD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_9283_cov_4.345687"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..713)
                     /locus_tag="P5079_06290"
     CDS             complement(78..713)
                     /locus_tag="P5079_06290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRY29633.1"
                     /translation="MAKLVLVRHGESQWNLENRFTGWVDVPLSDRGREEARRAGGHIK
                     DIKFDKAFTSALKRAQETLAIMMDEIGQKNLPVEKDQALNERHYGALQGLNKKETAAK
                     YGDQQVHIWRRSYDVPPPADKTELNPDGRSESLKDTAARTLPYFNARIMPEVKAGKNI
                     LVAAHGNSLRSIVMDLDRLTKEQVLELNIATGAPILYEVDAAGKILGKKVL"
     gene            complement(759..2420)
                     /locus_tag="P5079_06295"
     CDS             complement(759..2420)
                     /locus_tag="P5079_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29634.1"
                     /translation="MAEKINSRRSLLLPVFFFLALLPFVQRPLFFDDAAFFSFAKGAL
                     ASPLRPYDFSADLSLPDQPVWKFGSFAEFTHPPLVGWALAVPLGLFGEKSAPLHVAVF
                     LVAALVFFVGRSLMADFTKRPFFAVLFFAASPAFFLTSLTLYAQMFYLLFYLLTLWSF
                     QSLAGWKRWLGCGACLALAALSHHQWPALVLILGIRALSMRGAMDGAWRSAWIGAAVF
                     VIFYGGWSLWETKVYGMPNFVSSYRVRAVSAGGYPWRAAFLPFVFMAAAVPGVFYSWF
                     FIFKRGRRLGLFPGILLPLAALFFASPWGGFSSGQSTALAVFLSTGFCFLIAAVLFLK
                     GADAKDRFLVGWFLVEYGFLQRYLVFPSGHHLMMLAFPAVLLSVRMAEMCGVGRKFFW
                     WAGGIMSALTLFLAAADYSQACVGPRVAKDFPAPTERSFYWGNVFGAYGHHLKAAGWK
                     PYDRRVPPRAGDYVLVPRHVNAQGPSAHLRQYPFYAVHKLTYDQKIPVRTCSMVHLAG
                     WYSASWGVLPYVFAAGPVEEFMLLQCGRPKDVSGGTGHGRIPRSF"
     gene            complement(2452..3837)
                     /locus_tag="P5079_06300"
     CDS             complement(2452..3837)
                     /locus_tag="P5079_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY29635.1"
                     /translation="MGRFRRGQKIANRILAGVFFLTASFISLLPPETLHANGFYRNGI
                     GARSVGLGGAFIPLGEGPLGSMGSNPAGLGYLTYPTLDVSLVGVARDGILGAASSDPD
                     GRMYGSASALPEVAWARPMNKDNDGFGVGLAVIPESLWSAQWQYADPAGGMDGQTSYG
                     SQRNAVRMTVVRGSAGVGFSFGSVLAVGGSLGWVYNETVMQGPYVFQSTATGVGGFKT
                     LMDLRTNGVGINGSGGLLLRIHPRVQFGGAYVSKTVIHGKGDAYGNASAQLNSLGGVF
                     AGADPLFHYDLETLLVLPARAGGGLAVLPHKKWLVSAQADWYNWADAFDQLDIDFLRG
                     TNAALNALSGSDRLNEVYPFKWENRVVVRGGLEFSPTRHARFRAGYAQGKSPVPEEYL
                     TPLWAFLPERSASVGAGYRSEQVKCDFAYQMDFARAQTIQRTRLLAGENAGQRLRTVA
                     HTIAITLSTYF"
     gene            complement(3847..5034)
                     /gene="tyrS"
                     /locus_tag="P5079_06305"
     CDS             complement(3847..5034)
                     /gene="tyrS"
                     /locus_tag="P5079_06305"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY29636.1"
                     /translation="MNTDMKKILRGAQKVISETELAAKLKKGKPLRIKLGVDPTAPDL
                     HLGHTVSLTKLRQFQDAGHLVVFIVGDYTARIGDPSGRSETRPPMSPADIEKNAKTYL
                     DQVFKILDSTKTEVRRNSEWLAPLFANQGAAAGGNILSLLSHHTVQQLTERDDFAKRL
                     KEGIPISLLELLYPLMQGYDSVAIRADVELGGTDQLFNLLMGRQMQKDAGQEPQVVMT
                     LPLLEGTDGVKNMSKSYGNTVALNDASADMFGKIMAVSDDLMWKYYELLTDEEIPPLK
                     ALHPMEAKQRLAEQVVRRYHGGAAAQEARNAFVHVFSRRATPEKVSEHRLSRSPMPAA
                     ELLVESGLAPSKNEARRLLQQGAVELAGRRVNGDEAVSVAEPVLVKVGKRKFLKILPP
                     AGS"
     gene            complement(5031..5666)
                     /locus_tag="P5079_06310"
     CDS             complement(5031..5666)
                     /locus_tag="P5079_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29637.1"
                     /translation="MGMMLFGFVILAAVSAFAYFMRRRDDVLRFQAQILSVRFALETL
                     LRRRRELAGTWCSLCEERELMPDHIRRVRQALGRMAEAEQKGGNEFFPAGRLEDEKKL
                     SRLIRETYVHFLEQMPDDAAPRDFFRQYLQSLTRLEKEVSDAQELYNDCALVYNNALA
                     GWVGRLLRRWERWRPRPMISLLEPGAARHGGALRPGAAAAPAAPPIKDVNQ"
     gene            complement(5685..6791)
                     /gene="mnmA"
                     /locus_tag="P5079_06315"
     CDS             complement(5685..6791)
                     /gene="mnmA"
                     /locus_tag="P5079_06315"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRY29638.1"
                     /translation="MSRTALSQVVVAMSGGVDSSVAAALLQEEGHRVIGVTLRLLGGE
                     TAFGCCGSTRDIEDAKAVCARLGIPHYVLDFSDVFEEQVRDPFVRSYLKGETPNPCID
                     CNRYVKFDALFERALRLGADRVATGHYARIALVKKKAGAIYELRRAVDLKKDQSYVLH
                     HLGQKQLARLLFPVGELSKAEVRGEARRLKLRVADKPDSQEICFVPNADTAAYLRRCA
                     SDSERLRPGEIRDTAGRLLGNHRGVALYTRGQRHGLGLAAGKPMYVVDLRPESNTLVV
                     GTDEETLSPRFSVRDLHWTADSAPGRSFRAKVQIRSRHTAMACRVTVSAETGEVELNE
                     PQRAVTPGQSAVFYAGDKVIGGGTISSAVLSSAD"
     gene            complement(6788..8017)
                     /locus_tag="P5079_06320"
     CDS             complement(6788..8017)
                     /locus_tag="P5079_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRY29639.1"
                     /translation="MHTIYLDYNATTPMRPELLEAVRSYSGLEGDFGNPSSLHTAGQR
                     ARAALERAREQVAGFLGASDVSEIVFTSGGTEADNLAIQGAAFAHRDAGRHIITSSVE
                     HSAVMKTCRFLETQHGFEVTYLPVDRFGRVSPEDLRKALRRDTVLVSLMAANNEIGTV
                     EPLMELGAVCRERGVLFHSDAVQAAGRLPIDVKTWPVDMLAVSGHKIYAPKGIGALWL
                     RRGTRLQALQYGGSHEKNRRAGTENVAGAAALGLACELAGKELEGEVKMLRTLRDRLE
                     QGILARIPLTSVNGHPTERLCNTTHIAFECVEGESLLLALDQAGFSLRRPELPGICVS
                     TGSACSAGLLEPSHVLSAIGVPPERLHGCVRFSLGHYNTQQDVDQVLEIMPPIVEKLR
                     SLSPLWDEKRRGARSAG"
     gene            complement(8138..>9283)
                     /locus_tag="P5079_06325"
     CDS             complement(8138..>9283)
                     /locus_tag="P5079_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRY29640.1"
                     /translation="DMIGLSGLLVKSAQQMAVTAEDLKAAGIAVPLLLGGAALTEKFT
                     AVKIAPAYGAPAVYARDAMGGLEAANRLMGDREAFLERLKGGQEKLRAASPVSVSVPP
                     EKGSAPISRRYDPPVPPDLKLHVAEKFSVEEIFEHINPMMLYGKHLGLKGRLQKLLEA
                     KDETAVKLHGQVTALQDEILTEKYLLPKAVWRFYPAQSDGDRLILYRDIYGREAAAAF
                     DFPRQQAGERLCLSDFVLPKSAGAMDYVALFVVSVGGRATEQSAVWRERGDYFKSHAL
                     QAVAIESAEALAELLHEKIREMWGIDAGGLSLQEKFQAKYRGLRVSFGYPACPDLADQ
                     ARLFQLLQPEDKIGVRLTENFMMDPEASVSAMVFHHPQARYFSVLES"
BASE COUNT         1969 a         3102 c         2562 g         1650 t
ORIGIN      
        1 acctgaaaag ctggatgccc caggctcttc aggagtatga agcctccccg ttccgctgag
       61 cgccgggccg gcgcttctta aagaaccttt ttgcccagta tctttccggc ggcgtccacc
      121 tcatagagga tgggggcgcc ggtggcgatg ttgagttcca acacctgttc cttggtgagc
      181 ctgtccaaat ccatcacgat ggaacgcagg ctgttaccgt gggcggccac caggatgttc
      241 ttccccgcct tcacttcggg catgatcctc gcgttgaaat agggcagggt gcgcgccgcc
      301 gtgtccttaa ggctctcgct gcggccgtcc gggttcaact cggtcttatc cgccggaggc
      361 ggcacgtcgt agctgcgccg ccagatgtgc acctgctggt ctccgtattt cgccgccgtt
      421 tcctttttgt tcagaccctg cagcgcgccg taatgccgct cgttcagggc ctgatccttc
      481 tcgacgggca gattcttttg tccgatctcg tccatcatga tggccaaggt ttcctgggcg
      541 cgtttcagcg ccgaggtgaa cgccttgtcg aacttgatgt ccttgatatg gccgccggcc
      601 cggcgggctt cctcgcggcc gcggtccgat aacgggacgt ccacccaacc ggtgaaacgg
      661 ttctccaaat tccattgcga ttcgccgtga cgcaccaaca caagtttagc catacgcttc
      721 ccccgaaatt gaagtttgaa tggtgaacat tatcaaattt aaaaggaacg tggaatgcgc
      781 ccgtgaccgg ttccgccgga aacgtctttt ggccgtccgc actgaagcaa cataaactct
      841 tccaccgggc ccgcggcaaa gacgtaggga agaacgcccc agctggcgga ataccatccg
      901 gccaggtgca ccatggaaca tgtccgaacg gggattttct gatcgtaggt caatttatgt
      961 acggcgtaga acgggtattg tctcaaatgg gccgacgggc cctgcgcgtt gacatgccgt
     1021 ggcacgagga catagtcccc ggctcgcgga gggacccttc gatcgtacgg cttccacccc
     1081 gccgccttga gatgatgtcc gtaggcgcca aagacgttcc cccaataaaa agatctctct
     1141 gtgggtgccg ggaagtcctt ggcgaccctc ggtccgacac acgcctggct gtaatccgcc
     1201 gccgccaaaa acagcgtcag cgcgctcatg atccccccgg cccaccagaa aaatttccgc
     1261 ccgaccccac acatctccgc catccggacc gacaggagga cggctggaaa cgccaacatc
     1321 atcaggtgat gtccgctggg aaaaaccaga tatctctgta aaaaaccgta ttccacgagg
     1381 aaccatccga ccaggaaccg gtccttcgcg tccgctcctt tcaaaaacag gactgccgca
     1441 atcaaaaaac agaagcccgt cgacaaaaaa accgccaaag ccgtggattg tccagaggaa
     1501 aatccccccc aggggcttgc aaagaacaat gcggccaacg gcaaaagaat ccccgggaag
     1561 aggcccagcc gccggcccct cttaaaaata aaaaaccacg aatagaaaac accggggacc
     1621 gccgcggcca taaaaacaaa aggcagaaac gccgcccgcc acggataacc gcccgccgaa
     1681 acggcccgga ccctgtaact cgaaacaaag ttgggcatac cgtaaacctt cgtttcccaa
     1741 agggaccaac ctccgtagaa aatcacgaaa acggccgcgc cgatccaagc cgaacgccac
     1801 gccccgtcca tcgcccccct cattgaaagc gccctgatcc ccaagatcaa cacaagcgcc
     1861 ggccattgat ggtggctcag cgcggccaag gccaaacacg ccccgcaccc cagccaccgc
     1921 ttccatcccg cgagagactg aaaactccaa agcgtcagaa gataaaagag caaataaaac
     1981 atctgcgcat aaagcgtaag gctcgtcagg aaaaaagcgg ggctggcggc aaaaaacaga
     2041 acggcgaaaa atggccgctt cgtaaaatcc gccatcaacg aacggcccac gaaaaacacg
     2101 agcgccgcca caaggaatac ggccacatgc agcggggcgc tcttttcacc aaaaagaccc
     2161 aacggaaccg ccaacgccca tccgacgagc ggtgggtggg tgaattcggc gaatgacccg
     2221 aacttccaga ccggctggtc cggcaaagac aaatccgccg agaaatcgta gggcctcaac
     2281 ggcgaagcca aagccccctt ggcaaaggaa aaaaaggccg cgtcatcgaa gaacaacggg
     2341 cgctgaacga acggcaggag ggccagaaag aaaaagacgg gcaaaaggag ggaccgccga
     2401 ctgtttattt tctccgccac cgacgtcaga aacatcatct atggggaagc gctagaaata
     2461 cgtggagagg gtgatggcga tggtatgggc cacggtccgc agccgctggc cggcgttttc
     2521 gccggccagg agcctggtcc gctggatggt ctgtgcccgc gcaaaatcca tctgataggc
     2581 gaaatcgcat ttcacctgtt ccgagcgata cccggcgccg acggaagccg accgttccgg
     2641 caaaaacgcc cacaacggcg tgagatattc ttccggcacg gggctcttgc cctgcgcata
     2701 gccggcgcga aaccgggcgt gccgggtggg actgaactcc agcccgcccc gaaccacaac
     2761 gcggttctcc catttgaacg gatagacctc attcagccgg tcggaaccgg acagagcgtt
     2821 cagcgcggcg ttggtccccc gcaaaaaatc gatgtccaac tgatcgaagg cgtccgccca
     2881 gttataccaa tccgcctgcg ccgagacgag ccacttttta tgggggagca cggcgagccc
     2941 gccgccggcc cgcgccggaa gaaccagcaa cgtttccaaa tcgtaatgaa acagcgggtc
     3001 cgcccccgcg aaaaccccgc ccagggaatt caactgcgcg gaggcgttcc cgtaggcatc
     3061 gcccttcccg tggatgaccg ttttgctcac gtacgcgccg ccgaactgca cgcgcggatg
     3121 gatccgcaac aacagccccc cgctgccgtt gatcccgacg ccgttcgtcc tcaaatccat
     3181 caacgtcttg aacccgccca cccccgtggc cgtggactga aacacgtacg gtccctgcat
     3241 cacggtctcg ttatagaccc atcccaaact ccctcccacg gcaagaacgg aaccgaagga
     3301 aaaacccacg cccgcggaac cgcgcaccac cgtcatccgg acagcattcc gctgggaccc
     3361 gtaggaggtt tgaccgtcca tccctcccgc cggatcggcg tattgccatt gcgccgacca
     3421 caaagattcc gggatcaccg ccagaccgac cccgaacccg tcgttgtcct tgttcatggg
     3481 ccgggcccag gccacttccg gcaaggccga cgccgagccg tacatccggc cgtcgggatc
     3541 ggaagaggcc gcgcccagaa tgccgtcgcg ggccaccccg acgaggctca cgtcaagcgt
     3601 gggatacgtc aggtatccca gcccggcggg gttcgatccc atggacccca gcggtccttc
     3661 ccccagggga atgaaagccc ctcccaaccc gacggaacgg gcgccgatgc cgtttcgata
     3721 gaaaccgttg gcgtgaagcg tttctggcgg aaggagggaa atgaaggacg cggtaagaaa
     3781 aaaaacgccg gcgaggattc ggttggctat tttttgtcct ctccggaaac gacccatttc
     3841 aaaaaatcaa cttccggccg ggggaaggat tttgaggaac ttccgtttgc ccacctttac
     3901 caggaccggc tcggccaccg acaccgcctc gtccccgttc acccggcgac cggccaattc
     3961 cacggccccc tgctggagaa gtcgccgcgc ctcgttcttg gaaggcgcca aacccgactc
     4021 caccaggagt tccgccgccg gcatcggaga ccgggacagg cggtgttccg acaccttctc
     4081 cggcgttgcc cgtcgggaaa aaacatggac gaacgcgttt ctcgcctcct gagccgccgc
     4141 accgccgtga tagcgccgaa cgacctgctc cgccagccgc tgcttcgcct ccatgggatg
     4201 gagcgccttc agcggcggaa tctcttcgtc ggtcaaaagc tcatagtatt tccacatcaa
     4261 atcgtccgat acggccatga tctttccgaa catatccgcc gaggcgtcgt tgagggccac
     4321 ggtgttgccg taggacttgg acatattctt gacgccgtcg gtgccctcca ggagcggcaa
     4381 ggtcatcacc acctgaggtt cctggccggc gtccttctgc atctgccgtc ccatcaagag
     4441 attgaaaagc tggtcggtgc cgcccaactc cacgtcggcc cggatcgcca ccgagtcgta
     4501 gccctgcatc aacggataca acagttccag gagcgaaatg ggtatcccct ctttcagcct
     4561 tttggcgaaa tcgtcccgct ccgtcaactg ctgcacggta tgatgcgaaa ggagggacag
     4621 gatgttgccg ccggccgccg cgccctgatt ggcgaaaagc ggcgccagcc attccgaatt
     4681 ccgccggact tcggtcttgg ttgaatccag aattttaaat acctggtcga ggtaggtctt
     4741 cgcgttcttt tcgatgtcgg ccgggctcat gggcggacgc gtctccgaac ggccggaagg
     4801 gtctccgatc ctcgcggtgt aatcgccgac gatgaaaacg acgaggtgtc ccgcgtcctg
     4861 gaactggcgc agcttggtga gagataccgt gtgtccgagg tgcagatccg gcgcggtggg
     4921 gtccaccccc aatttgatcc ggagaggttt ccctttttta agtttggcgg ccagctcggt
     4981 ctcggaaatg actttttgcg cgccgcgcag gatttttttc atgtcggtgt tcattggttc
     5041 acgtccttta tcggcggcgc ggccggggcc gccgcggcgc ccgggcgaag agcgccgccg
     5101 tgccgcgcag cgcccggttc caggagagag atcatcggcc gggggcgcca cctctcccac
     5161 cgccgcaaca accggccgac ccaccccgcc agcgcgttat tgtagacgag ggcgcagtcg
     5221 ttgtaaagct cctgagcgtc gctgacctct ttctccaacc gcgtcaaaga ttggagatat
     5281 tgacggaaaa aatcccgggg ggcggcgtcg tcgggcatct gctccaaaaa atggacatag
     5341 gtctcccgaa taagccggct gagtttcttc tcatcctcca gccggccggc gggaaaaaac
     5401 tcattccccc ccttctgctc cgcctcagcc atgcgtccga gcgcctgccg gacgcgacgg
     5461 atgtgatccg gcatcaattc gcgctcttcg cagagggaac accatgtccc agccaactcc
     5521 cggcgacggc gcaggagggt ttccagggca aaacgcacgg aaagtatctg tgcctgaaac
     5581 cgcaacacgt cgtcgcgccg ccgcatgaaa taggcgaacg cgctcaccgc cgccaaaatg
     5641 acaaagccga acagcatcat ccccacggga ccgtctcctt ttccctaatc ggccgacgac
     5701 aggacggcgg acgatatcgt ccccccgccg atcaccttgt ctccggcata aaaaaccgcc
     5761 gactgtcccg gcgtcaccgc acgctgaggc tcgttaagct cgacctcgcc cgtttcggcc
     5821 gacacggtca cccggcacgc catcgccgtg tgccgggacc gtatctgcac tttggcccgg
     5881 aacgacctcc ccggcgccga atccgccgtc cagtgcaaat ccctcaccga aaaacgaggc
     5941 gacagcgttt cctcatccgt tcccaccacc aacgtgttgc tctccggcct caaatccacc
     6001 acatacatgg gcttccccgc cgccaacccc aacccatgac gctgtccccg cgtgtagagc
     6061 gccacgccgc ggtggttccc cagcaaacgg ccggcggtgt cccggatctc tcccgggcga
     6121 agccgttcgg agtcggatgc gcatcgacgg agataagccg ccgtgtccgc gttcggaacg
     6181 aaacaaattt cctgactgtc gggcttgtcc gccacgcgca gtttcagccg gcgggcttca
     6241 ccccgcacct ccgccttgga aagttcaccg acggggaaaa ggagtctggc cagttgtttc
     6301 tgccccaaat ggtggagcac ataggactgg tcttttttca ggtccacggc gcggcgcagc
     6361 tcatagatcg cccccgcctt ttttttcacc aaggctatgc gcgcgtaatg cccggtggcc
     6421 acccggtccg cccccaaccg gagcgccctc tcgaaaagag catcgaattt cacataacgg
     6481 ttgcagtcga tgcacggatt gggcgtctcc cctttcagat aggaccgcac gaagggatcc
     6541 ctcacctgtt cctcaaaaac gtcggaaaaa tccaggacgt agtgcggaat ccccagccgc
     6601 gcacagaccg ccttggcgtc ctcgatgtcc ctcgtggagc cgcaacagcc gaaggccgtc
     6661 tctcccccca gcagacgcag cgtcaccccg atgacacgat ggccttcttc ctgcaaaaga
     6721 gcggccgcca cggaggaatc cacccctccg gacatggcca ccaccacctg agaaagggcc
     6781 gtccgactca tcccgccgac cgtgcgccgc gccgcttctc gtcccacagg ggagacaaac
     6841 tccgcagctt ctccacgatg ggaggcatga tttccaggac ctggtccacg tcctgctgcg
     6901 tattataatg tccgagggaa aagcgcacgc atccatggag acgttccggg ggaacgccga
     6961 tggccgataa cacgtgcgac ggctccaaca ggccggccga acacgcggac cccgtcgaaa
     7021 cacagatccc cggcaactcc ggtcgacgca gggaaaaacc ggcttggtcc agcgccaaca
     7081 ggaggctctc cccttccacg cactcgaaag cgatgtgggt cgtgttgcag agccgttccg
     7141 tggggtgccc gttgaccgag gtcaacggga tcctcgccaa tatcccctgt tccaaacgat
     7201 cgcgcagagt ccgcaacatt ttcacttccc cctcgagttc cttccccgcc aattcacacg
     7261 ccagcccaag cgccgcggcg ccggccacgt tctccgtccc ggcgcggcgg tttttctcat
     7321 gcgagccgcc atactgcaac gcctgcagcc gcgtgccgcg gcgaagccag agcgcgccga
     7381 tccccttggg cgcgtagatc ttgtgtccgg aaacggccag catgtccacg ggccacgtct
     7441 ttacatcgat cggcagtctc cccgccgcct gcaccgcgtc ggaatggaac aataccccgc
     7501 gctcgcggca gacggccccc aattccatca acggttccac cgtgccgatt tcgttgttgg
     7561 cggccatgag actgaccagg acggtgtccc gtcgcaacgc ctttctcaga tcctccggag
     7621 aaacccggcc gaaccggtcc acggggaggt aggtgacctc aaaaccgtgc tgcgtttcca
     7681 ggaaccggca ggttttcatc accgcgctgt gttccaccga actggtaatg atgtgccggc
     7741 cggcgtcacg atgggcgaac gccgccccct ggatggccag gttatcggcc tcggttcccc
     7801 cggaggtaaa aacgatttcc gaaacatcgg aagcgcccaa aaaaccggcc acctgttccc
     7861 gcgcccgctc caacgcggca cgggcccgct ggccggccgt gtggaggctg gacggattcc
     7921 caaaatcgcc ctccagtccc gaataggacc gcacggcttc caaaagttcc ggccgcatgg
     7981 gcgtggtggc gttatagtcg agataaatcg tatgcaccgg gggtcctttt caatcgtcga
     8041 gttcgcgggg ggtccggcag gctacggctt ctttggaaat tttaacactt ttcccacaaa
     8101 gaatattcgt ccggagcgga aatcagcgga acgtcaatca ggactccaat acgctgaaat
     8161 accgggcctg ggggtgatga aaaaccatcg ccgacacgct ggcctccgga tccatcatga
     8221 aattttccgt caaccggacg ccgattttgt cctccggctg aaggagttgg aaaagtctgg
     8281 cttggtccgc aaggtcgggg caggcgggat acccgaaact gacccggagc ccgcgatatt
     8341 ttgcctgaaa cttctcctgg agggaaagcc cccccgcgtc gatgccccac atctccctga
     8401 ttttttcatg cagaagctcc gccaacgctt cggcgctctc gatggccacc gcctgaagcg
     8461 cgtgggattt gaagtaatcc cccctttccc gccaaacggc ggactgttcc gtggcccggc
     8521 cgccgacgga aaccacgaag agcgccacat agtccatcgc gccggcggat ttcggcagaa
     8581 cgaaatcgct gagacacagg cgttcgcccg cttgttgccg tgggaaatcg aatgcggcgg
     8641 ccgcctctct cccatagata tctcggtaaa ggatcaggcg atctccgtcg gactgagcgg
     8701 gataaaaccg ccacacggct ttcggcaaca gatatttttc ggtcaatatc tcgtcctgca
     8761 aagccgtcac ctgcccatgc agtttcaccg cggtctcgtc cttggcctcc aacagtttct
     8821 gaagccggcc tttcagtccc aaatgtttcc cgtacagcat catcgggttg atgtgctcga
     8881 aaatctcttc gacggaaaac ttctccgcga cgtgcagctt caaatccgga ggcaccggcg
     8941 gatcgtatcg gcgcgatatc ggcgcggatc ctttttccgg gggaacggaa accgagaccg
     9001 gcgaggcggc cctcagtttt tcctgccctc ccttcaggcg ttcgaggaac gcttccctgt
     9061 cccccatcaa acggttggcc gcctccaggc cgcccatggc gtctcgggca taaaccgccg
     9121 gggcgccgta agccggagcg atcttcaccg cagtaaactt ttccgtcaac gcggcgccac
     9181 ccagcaacag cggcacggcg atccccgcgg ccttcaagtc ctccgcggtc accgccatct
     9241 gctgggccga tttcaccaga agccccgaaa gaccgatcat gtc
//
LOCUS       DAOTFD010000085         9273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5272_length_9273_cov_4.071708, whole genome shotgun sequence.
ACCESSION   DAOTFD010000085 DAOTFD010000000
VERSION     DAOTFD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5272_length_9273_cov_4.071708"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="P5079_06330"
     CDS             complement(<1..1388)
                     /locus_tag="P5079_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY29641.1"
                     /translation="MLHENLNQMLADAVKQSGDAAAFLFKDEPILYRDLDDKIRRCAA
                     GLAALGIGKDDTFGIVLRNCPEFVIAFFALSRLGAVAVPVNFLEKGERLGYIFHDAGV
                     KGCLTAREFLGNVREAQKRVPTLKHLFLKTAGHDLPAFDSLLHQGDPPPEHPVAPNDL
                     AMLIYTAGTTGSPKGVMLTHKNFVANVESCRKAIELSSADRFMCVLPMFHSFAWTTNV
                     LLPLRLGAGTVIIESLLPFEPVLHTIWKHNVNVFCGVPPIFSTLTQKVKGLKAMALRL
                     INPVRLVVSGAAPLPAGVQKKFEETFGTPLLEGYGLTEAAPVVSLNPLHGARKDGSVG
                     LPLPDVRVKIVDDEERLLPDGQVGEICVKGDNVMAGYYKRPQETREAFTKDGWLKTGD
                     LGLLEADGYLRIVDRKKDLIIVKGLNVYPQEVETVLRSHPAVDEAAVVGIYDSESGDE
                     TVKAFVTLKRDAA"
     gene            complement(1390..2190)
                     /locus_tag="P5079_06335"
     CDS             complement(1390..2190)
                     /locus_tag="P5079_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY29642.1"
                     /translation="MTPLLRAENLSMEFAVTGGVFRRRLGAVRALHNVSFSLNEGESL
                     GLVGESGSGKSTLGRILCRLHRPTSGRLFWDGRPMEDLPPLEWASGIQMIFQDPAASL
                     NPKLSVETILAEPLGLRARVEKKEPPRGAARRDAVRELLSSVGLPTDILSHYPHQFSG
                     GQKQRLAIARALAVRPRVLVADEPVSAVDLSLQAQILNLLSDLKEQYRLTTLIISHDL
                     TVVAHLADRLLVLKDGETMETGGTAEILAETRHPYTRALLDAVPPLFR"
     gene            complement(2187..3164)
                     /locus_tag="P5079_06340"
     CDS             complement(2187..3164)
                     /locus_tag="P5079_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29643.1"
                     /translation="MADPLLKIENLSVDYRRHGKRVHAVRDVSLELNEGETLGVVGES
                     GCGKSTLAMAVLRLLPDRDSRIPGGKIFFRSQDLLALDAEPLRRIRGGQIAVIFQDPF
                     SALNPVLTVGDQLEESMEVHGGGRDRAKTLSLLERVRLPDPERIYRAYPHQISGGQRQ
                     RACLALAIANRPALLIADEPTTALDVTLQKEILDLLDGLRRDLKMAILFITHNMGLVS
                     QRTDRLAVMYAGEIVERGKTADVMSAPLHPYTAALLRSLPRLHKSAGKLPIIPGQPPD
                     LSAVPGGCPFHPRCPRVFDPCAEHIPALLPHEGRSVSCHLYHSPVAAGR"
     gene            complement(3168..3998)
                     /locus_tag="P5079_06345"
     CDS             complement(3168..3998)
                     /locus_tag="P5079_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29644.1"
                     /translation="MWSLTLQRFRKNKLALAGSAILALLVLLALLAPVLVAMDPLQQD
                     VVQRLRPPSWSHWMGTDDLGRDVFSRVLFGTRISLVVGIVAVGLALLIGTSAGLVAGF
                     YGGWVDTLFMRLIDVLLCFPAIFLILMVIAFLEPNILNVVVIIGLTSWMGLARLVRGE
                     CLSLRERDFMSAARALHLSTPRLLFVHLLPNVASPVLVAATLGVGGAILTESALSFLG
                     LGVQPPTASWGNILAVGKDYIHIAWWLSLFPGLAILVTVLAFNLLGEGLRDVLDPRMD
                     "
     gene            complement(4103..5080)
                     /locus_tag="P5079_06350"
     CDS             complement(4103..5080)
                     /locus_tag="P5079_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29645.1"
                     /translation="MPAYFAKRLLFLVPLLVGITLVTFGVMQLAPGKPTDMVTDMNVK
                     ASAEAKERLIKLYGLDKPWHVQYWDWLKRFARLDFGRSFRDDRPVLRKIGERLPATLL
                     LNVSSLLLIFLIALPIGILSAVKQDSLWDKSLTVFVFIGFSVPTIWLALLAMITFGLK
                     LGWLPISGLRSLTYDEMSLGAQCLDLARHLALPVLVSAFTGLAGLSRYVRNGLLEVIR
                     QDYIRTARAKGLPENRVILKHALRNTLIPVVTLVGLLLPDLIGGSFIFETIFAYPGMG
                     RLGFDAIMARDYPVIMGVGTIAALLTLVGNFLADVAYAYVDPRIRYQGK"
     gene            complement(5086..6750)
                     /locus_tag="P5079_06355"
     CDS             complement(5086..6750)
                     /locus_tag="P5079_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HRY29646.1"
                     /translation="MSDGKQTLGSTLRRIVFCSTPVALSSLLFLSACRSDSIPQPAAS
                     ATDAAPATGDAYVEASIADASYLNPLLASDSASGNICSLVYNGLVKYDKDLRLVGDLA
                     ESWEVRSGGTEILFHLRPDVRWHDGAPFTADDVRFTYQRLTDPAVKTPYGVDFELIRR
                     VDVPDPHTVRVVYKEPFVPALESWGMGIVPKHIFSAGDFNTHPANRRPIGTGPYKFLE
                     WKTDEKIVLTANPDYFEGRPRLDRYIFRIIPDSSVQFLELRQQSIDSMALTPDQFKAY
                     PEFFRHYNKYRYPAFAYTYLGFNLENPLFRDRRVRRAVALALDRSEIIDGVLLGVGRP
                     ATGPFPPTSWAFNPDVADIPFDPVKAKALLATAGWTDTDGDGWLDRGGQKFEFTLMTN
                     QGNKPRELCALILQNHLAKIGIRVQVRILEWSSFLHNYVDKKRFDAVILAWSLSRDPD
                     QYSIWHSSQRKEGQYNFVGYANREVDMLLERGRREFDFEKRRALYHRMHAILAEDLPY
                     IFLYYPDSLPTIHTRFVGPEVAAAGIGWNFREWFVPKSLQKYRYAS"
     gene            complement(6747..8051)
                     /locus_tag="P5079_06360"
     CDS             complement(6747..8051)
                     /locus_tag="P5079_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY29647.1"
                     /translation="MKRRILCLILLTMMPLLKTQAATTSAKKMPNGLLWIHRRVTHNQ
                     IVAFHLFFPGGSSLEPAAKAGITALTASVMFKGTVSRSALRIAQEAESLGAALDSQSA
                     NDYWEVSGQSVAENFNGLFDLFQDVLLHPSFPAEEFAKEKAAHLNDIRAKKEHIFEVA
                     YDRLQKETFGDHPYARPEEGTESSVGSLTRDDLVDWHRTTVSPNGAVLVTVGNLPAKK
                     MESLLRRAFAGWTAAGETPAAPGAPVYNNAPVSAEEPHPFEQSYLMLAYPAPGVDDPS
                     YPAVKVLNAYLGAGMSSPLFQAVREQGSLAYEVSSFYGSRKWGSAFVIYAGTDPSTLN
                     DAERRMAHVLKNILQRPLTPEELADTQRYIRGHYMMDHQTNSRMAWYLGWWEMLEKKH
                     EHDARYPADVAAVSVEDVRRAARTIFSRPAVRVVIRSQKKKS"
     gene            complement(8085..>9273)
                     /locus_tag="P5079_06365"
     CDS             complement(8085..>9273)
                     /locus_tag="P5079_06365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY29648.1"
                     /translation="LQIYVRCGAVNETDASAGVSHFLEHMIFKGTDRLDAGKIAQVVE
                     RRGGSINAATGTEMTHYYVDMPSDSLDEAFEVLTESVLRPAFPPEELEKERRVILEEI
                     KRRNDNPESDLWDAFLETLYRETPYRRQVIGTAASIAAMPRDTLFRQHHDYYVPENMV
                     VVVAGDLRRATVEKKIKLAFGAVPARTAPARPPLVEPAPSNTVLKFISRPAQQAHVAV
                     GFLGPTLQDPEQVAMDVLATALGGGQSSPLYQILREQKRAVWSVGASFLTHAGSGVFG
                     IFAECPPEKARSLSNEIYFILDDALGNGLPPEALARAKAQLRSAWLFSQETYHGQAAQ
                     WGFYTILDRPQLARNYLKELDKVTLADLHRLLLACFQRRELSGAVLLPETSGGDAPRP
                     AAP"
BASE COUNT         1612 a         2581 c         3460 g         1620 t
ORIGIN      
        1 gcggcgtcgc gtttcagggt gacgaacgct ttgacggttt cgtcgccgct ttcgctgtcg
       61 tagatcccca ccacggcggc ctcatccacc gcgggatgcg aacggaggac cgtctccact
      121 tcctgcgggt acacgttgag ccccttgacg atgatgaggt ccttctttcg gtccacgatg
      181 cggaggtatc cgtcggcctc gaggagtccg aggtcgccgg ttttcagcca accgtccttg
      241 gtgaaggcct cgcgggtctc ctgggggcgc ttgtagtagc cggccatgac gttgtccccc
      301 ttgacgcaga tctcccccac ctgtccgtcg gggaggagcc tttcctcgtc gtccacgatc
      361 ttcacccgca cgtcggggag gggcagtccc accgagccgt ccttgcgcgc gccgtgcaag
      421 gggttgagcg acaccaccgg ggcggcttcg gtgaggccgt atccttccag gagcggcgtg
      481 ccgaacgtct cttcgaattt tttctgcacg ccggcgggca ggggcgcggc gccggacacc
      541 accagccgca ccgggttgat gagccggagg gccatggcct tgaggccttt caccttctgc
      601 gtgagcgtgg aaaagatggg cggcacgccg cagaagacgt tgacgttgtg tttccagatg
      661 gtgtggagca ccggctcgaa cggcaggagg gactcgatga tgacggtgcc ggcgccgagc
      721 cgcaggggca gaaggacgtt ggtggtccag gcgaaggagt ggaacatggg gaggacgcac
      781 atgaaccggt cggcggagga cagctcgatg gccttgcggc aggattcgac gttggcgacg
      841 aaatttttat gtgtgagcat gacgcccttg ggggagccgg tggtgccggc ggtgtagatg
      901 agcatggcca ggtcgttggg cgccacgggg tgttccggcg gaggatcgcc ttggtgaagc
      961 aggctgtcga aggccggcag gtcgtggccg gccgttttga ggaacaggtg tttgagggtg
     1021 gggacgcgct tttgggcctc ccggacgttg ccgaggaatt cgcgcgcggt gaggcagcct
     1081 ttcaccccgg cgtcgtggaa gatatagccg agcctctcgc ctttttccag gaaattgacg
     1141 ggcacggcca ccgcgcccag gcggctgagg gcgaaaaagg cgatgacgaa ctccgggcag
     1201 ttccgcagga cgatgccgaa ggtgtcgtcc ttgccgatgc ccagcgcggc cagccccgcg
     1261 gcgcagcggc ggatcttatc gtcgaggtcc cgatagagga tcggttcgtc cttgaagagg
     1321 aaggcggcgg cgtcgcccga ttgtttcacg gcgtcggcca gcatttggtt caggttttcg
     1381 tggagcatgt cagcggaaga ggggcgggac ggcgtccaac agcgcccggg tgtagggatg
     1441 ccgcgtttcc gccaatattt cggcggtgcc gccggtttcc atcgtctcgc cgtctttcag
     1501 caccaggagg cggtccgcca ggtgggcgac cacggtgagg tcgtggctga tgatgagggt
     1561 ggtgaggcgg tattgctctt tcaagtcgga cagcaggttc aggatttgcg cctggaggga
     1621 aaggtcgacg gcggagacgg gctcgtcggc caccagcacg cgcggccgga cggccagcgc
     1681 ccgggcgatg gccagccgct gtttctgtcc gccggagaac tggtgcgggt aatgggacag
     1741 gatgtcggtg gggaggccca cggaggagag gagttcccgg acggcgtcac gccgcgcggc
     1801 gccgcgcggc ggttcttttt tttcgacgcg ggcgcggaga ccgaggggtt cggcgaggat
     1861 cgtttccacg gaaagttttg ggttgagcga ggccgccggg tcctggaaga tcatttggat
     1921 gccggaggcc cactccagcg gcggcaggtc ttccatggga cggccgtccc agaaaaggcg
     1981 gccgctcgtg gggcggtgga ggcggcagag gatgcggccg agggtgctct tgccgctgcc
     2041 cgattccccg acgaggccga gggattcccc ctcgttcagg ctgaaggaaa cgttatggag
     2101 ggcgcgcacg gcgccgaggc ggcgccggaa cacgccgccg gtgacggcga actccatgga
     2161 aaggttttcg gcgcggagca ggggcgtcat cggccggccg ccacggggga atggtagagg
     2221 tggcaggaaa cgctgcggcc ttcgtgcggg agcagcgcgg gaatatgttc ggcgcagggg
     2281 tcgaacacgc gcgggcagcg cgggtggaac ggacaaccgc cggggacggc gctcaggtcc
     2341 ggcggctggc ctgggatgat gggcagtttt ccggcgcttt tgtgcaggcg gggcagggag
     2401 cggagcaggg ccgcggtgta cggatgaagc ggggcggaca ttacgtcggc ggtcttcccc
     2461 cgctcgacga tctcgccggc gtacatcacg gccaggcggt cggtgcgttg ggacaccagc
     2521 cccatgttgt gggtgatgaa gaggatggcc atcttcaggt cgcggcggag gccgtcgagc
     2581 aggtcgagga tctccttctg cagggtgacg tccagcgcgg tggtgggttc gtcggcgatg
     2641 agcagggccg gccggttggc gatggccagg gcgaggcagg cccgctggcg ttgtccgccg
     2701 gaaatttgat gggggtaggc gcggtagatg cgttccggat cgggcaggcg cacgcgttcc
     2761 agcagggaca gggttttggc gcggtcccgt ccgccgccgt ggacctccat cgattcctcg
     2821 agctggtcgc cgacggtcag caccggattg agggccgaaa aggggtcctg gaagatcacg
     2881 gcgatctgtc cgccgcggat ccgccggagg ggttcggcgt cgagggcgag gaggtcctgg
     2941 gatcggaaaa atatctttcc gcccgggatt cggctgtccc ggtccggcag gaggcggagc
     3001 accgccatcg ccagggtgga cttgccgcag ccggattctc cgaccacgcc gagggtttcc
     3061 ccctcgttca gttccaacga cacgtcgcgc acggcgtgga cgcgtttccc gtgccggcgg
     3121 tagtccaccg ataaattttc gattttcagc agggggtcgg ccatcggtca atccatgcgg
     3181 ggatcgagca cgtcgcgcag gccttcgccg agcaggttga aggcgagcac ggtgacgagg
     3241 atggccaggc ccggaaagag ggacagccac cacgcgatgt ggatgtagtc cttgccgacg
     3301 gcgaggatgt tgccccagga ggcggtgggc ggctggacgc cgaggccgag gaaggaaagg
     3361 gccgactcgg tgaggatggc cccgcccacg cccagggtgg cggccaccag caccggcgag
     3421 gccacgttgg gcaggagatg gacgaaaagg aggcgcggcg tggagagatg cagcgcccgc
     3481 gccgcggaca tgaagtcccg ttcgcgcagg ctgagacatt ccccccgcac caggcgggcc
     3541 agccccatcc aggaggtgag gccgatgatg accaccacgt tgaggatgtt gggttccagg
     3601 aaggcgatca ccatgaggat gaggaagatg gcggggaagc aaaggaggac gtcgatgagg
     3661 cgcatgaaga gggtgtccac ccagccgccg tagaacccgg cgacgagtcc ggcggaggtg
     3721 ccgatgagga gggccagccc gacggccacg atgccgacga ccaaagagat ccgcgtgccg
     3781 aagaggaccc gggagaacac gtcgcggccg agatcgtcgg tgcccatcca gtgcgaccac
     3841 gacggcgggc ggaggcgctg gaccacgtcc tgctgcaggg gatccatggc caccagcacg
     3901 ggcgccagga gcgccaacag caccaacagc gcgaggatgg cgctgccggc cagggccagt
     3961 ttgttcttcc ggaaccgctg aagggtgagg gaccacatat cagaaatctg aaatccgaaa
     4021 gacgagatgc gaaatggaaa acaaaaagtc ggaacgcata acgccttttt cgtatttcgt
     4081 attttgtatt tcgtatttca tgtcatttcc cttggtaacg gatgcgcggg tcgacgtagg
     4141 cgtaggccac gtcggcgagg aagttcccca cgagcgtgag gagggcggcg atggtcccca
     4201 cgcccatgat gacgggatag tcgcgggcca tgatggcgtc gaaacccagc cggcccatgc
     4261 ccgggtaggc gaagatggtt tcgaagatga aactgccgcc gatgaggtcg ggcaggagga
     4321 ggccgacgag ggtgacgacg gggatgaggg tgttgcgcag ggcgtgtttg aggatgacgc
     4381 ggttttccgg cagtcccttg gcccgcgcgg tgcggatgta atcctgtcgg atgacctcca
     4441 gcagaccgtt gcgcacgtag cgcgagaggc cggccagccc ggtgaacgcc gagacgagca
     4501 ccgggagggc caggtgccgg gccaggtcga ggcattgggc gccgagggac atttcgtcgt
     4561 aggtgagcga gcgcaacccc gagatgggca gccagccgag cttgagcccg aaggtgatca
     4621 tggccaggag cgccagccag atggtcggca cggaaaaacc gatgaagacg aacaccgtga
     4681 gcgacttgtc ccacagcgaa tcctgtttca ccgccgagag gatgccgatg ggcagggcga
     4741 tgaggaagat gagaaggagc gaggagacgt tcaacaggag cgtggccggg agtcgttcgc
     4801 cgatcttccg cagaaccggg cggtcgtcgc ggaaggatcg gccgaaatcg agacgggcaa
     4861 aacgtttgag ccagtcccag tactgcacgt gccagggttt gtccagcccg taaagtttga
     4921 tgagccgttc cttggcctcg gcggaggcct tcacgttcat gtcggtcacc atgtcggtgg
     4981 gtttgcccgg cgccagctgc atgacgccga aggtgaccag cgtgatgccg acgaggagcg
     5041 ggaccaggaa aaggagccgt ttggcgaagt aggcgggcat ggccgtcagg aggcgtatcg
     5101 gtatttctgc agggatttgg gcacgaacca ctcgcggaag ttccagccga tgcccgccgc
     5161 ggccacctcg ggcccgacga accgtgtatg gatggtgggt agggaatcgg ggtaatagag
     5221 gaagatgtag ggaaggtctt ccgccaggat ggcgtgcatg cggtgataga gcgcccggcg
     5281 tttttcgaag tcgaactccc gccggccccg ttccaggagc atgtcgacct cgcggttggc
     5341 gtagcccacg aaattatact gtccctcctt gcgctggctg gaatgccaga tggaatattg
     5401 gtccggatcg cgggagaggc tccaggcgag gatgacggcg tcgaaccgtt ttttgtccac
     5461 gtagttgtgg aggaaggagg accattcgag gatgcggacc tggacgcgga tgccgatctt
     5521 ggccaggtgg ttttggagga tgagggcgca aagttcgcgc ggtttgttgc cttggttggt
     5581 catgagggtg aactcgaatt tttgtccgcc gcggtcgagc caaccgtcgc cgtcggtgtc
     5641 ggtccagccg gccgtggcca gcaaggcctt cgccttcacg ggatcaaaag ggatgtccgc
     5701 cacgtccgga ttgaacgccc aggaggtggg cgggaacggc ccggtggcgg gccggcccac
     5761 gcccagcagg acgccgtcga tgatctcgga gcggtcgagc gcgagggcga cggcccgccg
     5821 cacgcgcctg tcgcggaaca gcgggttttc caggttgaac cccaggtagg tgtaggcaaa
     5881 cgcggggtag cggtatttgt tgtagtggcg gaagaattcg ggataggcct tgaactggtc
     5941 cggcgtgagc gccatggagt cgatggattg ctggcggagt tcgaggaact gcaccgaact
     6001 gtcggggatg atgcggaaga tgtagcggtc gaggcgcggc cggccctcga aatagtcggg
     6061 gttggcggtc agcacgatct tctcgtcggt cttccattcc aggaacttgt aggggccggt
     6121 gccgatgggg cggcggttgg ccggatgcgt gttgaagtcg ccggcggaga aaatgtgttt
     6181 ggggacgatg cccatgcccc aggactccag cgccggcacg aaaggttcct tgtagaccac
     6241 gcgcacggtg tgcgggtccg gcacgtccac ccggcggatg agttcgaagt ccacgccgta
     6301 gggcgttttg acggcggggt ccgtgaggcg ctggtaggtg aaccgcacgt cgtcggcggt
     6361 gaacggcgcg ccgtcgtgcc agcggacgtc ggggcggaga tggaagagga tctccgtgcc
     6421 gccggaccgg acctcccagg actcggccag gtcgccgacg agccgaaggt ccttgtcgta
     6481 tttcaccagc ccgttgtaaa ccagggagca gatgttgccg gaggcggagt ccgaggccag
     6541 caaggggttg agataggagg cgtccgcgat ggaggcttcc acataggcgt cgccggtggc
     6601 gggggcggcg tcggtggccg aggcggccgg ctgggggatg gaatcggacc ggcaggcgga
     6661 gagaaacagc aaggaggaca gagcgacggg ggtcgagcaa aagacgatcc ggcgcagggt
     6721 cgagcccagc gtttgtttcc cgtcgctcat gactttttct tttgcgaccg gatgacgacg
     6781 cgcaccgcgg gccgggagaa aatagtccgc gccgcccgcc gcacgtcctc caccgaaacg
     6841 gccgccacgt cggcgggata gcgggcgtcg tgttcgtgtt ttttttcgag catctcccac
     6901 cagcccagat accacgccat gcggctgttg gtctggtggt ccatcatgta atgtccccgg
     6961 atgtaacgtt gggtgtcggc cagttcctcc ggagtgaggg gccgttgcag gatatttttc
     7021 aggacgtgcg ccattcggcg ttcggcgtcg ttcagcgtgg aggggtcggt gccggcgtag
     7081 atgacgaagg cgctgcccca tttgcgcgat ccgtaaaagg aggagacttc ataggcgagg
     7141 ctgccctgtt cccgcaccgc ctgaaaaaga ggcgagctca tgcccgcgcc gaggtaggcg
     7201 ttcaagacct tgacggcggg ataggagggg tcgtccaccc cgggggccgg gtaggccagc
     7261 atgagatagg actgttcgaa cgggtgcggt tcctcggccg agacgggcgc gttgttgtag
     7321 acgggagccc cgggggcggc gggcgtttct ccggcggccg tccacccggc gaaggcgcgg
     7381 cgcagcaggg attccatttt tttcgccggg aggttcccca ccgtcaccag cacggcgccg
     7441 ttgggcgaga cagtggttcg gtgccaatcg acgaggtcgt cgcgggtgag ggaaccgacg
     7501 gaggattccg tcccttcttc cggccgggcg tagggatggt cgccgaaggt ctctttttgg
     7561 aggcggtcgt aggccacttc gaagatgtgt tccttcttcg cccggatgtc gttgaggtgc
     7621 gcggcctttt ccttggcgaa ttcctcggcg gggaaggagg ggtgcagcag gacgtcctgg
     7681 aaaaggtcga aaagcccgtt gaaattttcc gcgaccgact ggccggacac ctcccaatag
     7741 tcgttggcgc tctgggagtc gagggcggcg ccgagggatt cagcttcctg cgcgatacgc
     7801 agggcggaac gcgagacggt gcctttgaac atcaccgagg cggtcagcgc ggtgatgccg
     7861 gctttggcgg ccggttcgag cgaggagccg ccggggaaga aaagatggaa cgccacgatc
     7921 tggttgtggg tcacgcggcg gtggatccac aacagaccgt tgggcatttt tttcgccgag
     7981 gtcgtggcgg cctgtgtctt cagtaagggc atcatcgtca gcaggatcag acagagaatc
     8041 cgtcgtttca cggagccgtc cgttttaaga acgccgccgc cgggtcacgg cgcggccggc
     8101 cgcggggcgt cgccgccgga ggtttccggc aggagcaccg cgccggaaag ttcccggcgc
     8161 tgaaaacagg ccaggagcaa ccggtgcagg tcggccaacg tgaccttgtc cagttctttc
     8221 aggtaattcc gcgccagttg gggacggtcc aatatggtgt agaaacccca ttgagccgcc
     8281 tggccgtgat aggtttcctg ggagaaaagc caggcgctgc gcaactgcgc tttggcgcgg
     8341 gccagggctt ccggcgggag gccgttgccg agggcgtcgt cgaggatgaa atagatttcg
     8401 ttggacaggg aacgggcttt ttccggcgga cattcggcga aaatgccgaa cacgccggag
     8461 ccggcgtggg tgaggaaact ggcgccgacc gaccacacgg cgcgtttttg ctcgcgcaat
     8521 atctgataga gcggcgagct ttgtccgcct cccagcgccg tggccagcac atccatggcc
     8581 acctgttccg ggtcttggag ggtggggccg aggaacccca cggccacgtg ggcctgctgg
     8641 gccggccggg aaatgaattt gagcacggtg ttcgacgggg ccggctccac gagcggcggt
     8701 cgggcggggg cggtccgggc cgggacggcg ccgaacgcga gtttgatctt tttttcgacg
     8761 gtggcccgtc gaaggtcacc cgccacgacc accaccatgt tctccggcac gtaataatcg
     8821 tggtgctggc ggaagagcgt gtcgcgcggc atggcggcga tgctggcggc ggtgccgatg
     8881 acctgtcgac ggtagggcgt ttcacggtag agggtttcca ggaaggcgtc ccagaggtcc
     8941 gattcggggt tgtcgttgcg gcgtttgatc tcttccagga tgactcggcg ttccttttcc
     9001 agttcttccg gcgggaaagc ggggcggagc accgactcgg tcagcacctc gaaggcctcg
     9061 tcgaggctgt cggagggcat gtccacgtaa tagtgcgtca tttcggtgcc ggtggcggcg
     9121 ttgatggatc cgccgcggcg ttccaccacc tgcgcgatct tcccggcgtc gaggcggtcc
     9181 gttcccttga agatcatgtg ttcgaggaaa tgcgagactc cggcggaggc gtcggtctcg
     9241 ttgacggcgc cgcagcgcac gtagatctgc agg
//
LOCUS       DAOTFD010000086         9261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5279_length_9261_cov_3.891810, whole genome shotgun sequence.
ACCESSION   DAOTFD010000086 DAOTFD010000000
VERSION     DAOTFD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5279_length_9261_cov_3.891810"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="sat"
                     /locus_tag="P5079_06370"
     CDS             <1..527
                     /gene="sat"
                     /locus_tag="P5079_06370"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395084.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRY29649.1"
                     /translation="DGLFLHPLVGATKEDDVPAAVRMRCYETLLKNYFPAGRTLLAVN
                     PANMHYAGPREALFHALVRKNYGCTHFIVGRDHAGVGNYYGPYDAQKIFSNFAPDELD
                     ITPLFFDNAFWCRRCAGMSSEKTCPHAPEDRLFLSGSRLREMLQRGERPPAEFSRPEV
                     IDILLESFSKQTRS"
     gene            695..>9261
                     /locus_tag="P5079_06375"
     CDS             695..>9261
                     /locus_tag="P5079_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY29650.1"
                     /translation="MPKPRLFFPVSFVRATSLVTLIVFVFSTLAVPYSEASFWSERRR
                     AAENIVKESDSPSAVLAQLPRVASRDLSALSALPPALKSVVDASSVGDSDLPPPAAAP
                     WMRSLLSSYGEVRDAFRAPQEKSWVVLLEDLHTSTEAQENISGLIRRIADEFPASSPS
                     TEGLTIGVEGAWGDFDLAGHRLFADPEARRPVCDTLLNSGVLTGAEHAALTVEHPVRL
                     LGLEDPALYRENIQAYKDSRATAPAFEAISKQYGRLLDQTKKNHYSAALLGFDGEAIA
                     YSRGSRSLGQFIETMRGTVLDDAWMRRYPTAAGFRQVLALEKSLDFQRVERERNEIIS
                     GLVESLSDKELAEFTASALAYRLGEITYTAFYTGLRRLGEAHGVSWTDYPALDDYFRY
                     IYLAARLDRDRLYREVDEWRDELYGRLLTTPEEKRIHSLSEDFVLVTKLAAFSLNSAE
                     WGRACQRHDVFASLPKRFEEWGGSSTDASAWEKQLESFEKFYRIAERRNAALVDTLAR
                     SQRRGPPAVQLMVLGGFHSEAVKEILRGRGMSWVVIRPNVTRAGDAPHYMDLFARART
                     PLEKLFAGDALFLRSPLALSREVPTGYAHLRVTEQGLYVSLSTAKTLLKTLREMGAST
                     PSQRRVALQSVVARLQEAARRFDALGDKLVLRLEGTEGGTLTFSVQYEGPRLEQRQSV
                     VLRVQAGGTEPAGALKMTVGEDPVFLTPTKGLRQVFQRGMREVKSFWNRIVRPAFRLK
                     QLGPYPLKEKIHHQVYVGQDPTTLEPVVLKAPDKKIPGFQQAPAETLLGREARVLDFI
                     QKNAPPEERSSFPKLLTDGVRIDEPTRLPYIAMEFVDGQTLDDVVTKGQIEPEERLGL
                     AAEILRALAILHSIGVVHNDFKPTNVMVTRNGEPRIKLIDFEISAVKGLSLEGDAMAA
                     QIGAIPYMAPERLGRGPSTSEADVYAAGVVLYELLTGRLPFDFWLAESEDDYSFWLDW
                     MMKERAKTPKFLEDDDPRFPPEIKALLKRMLAMDPQDRPTASEAVSLMEGLSVTARKS
                     MPPPAAFAETAGRRGATLRWLMPLGMWLAKKIGVEFKELPAGADYRTLSPESRARFDK
                     EYGAKVAWALEETAVSLLTVALQFLPGFSMHLVLAYASVRILFVLLHVPDWTGPPTLK
                     NFYDQLGAPLIVSLVPAAAFGLAHVVSLPVSLVFAFNILLGFLAHRVVNLGVYEGWRA
                     FPARPASLFSFGSRTAFELEMDAARLSARKIPSRVLELIQTSEPIGRGAEACVYRIPE
                     MPSIVARVPVQMADEMRGAPFGHLWAKRLLEEMRQGRKCDDFVKNAAGAAIDEKNVRP
                     LLNGIIASRDFTSSVDLDLIRFDDFVGPLARKYKSGVRLTDSELRVMNRYLLVKLYPV
                     TLKDYIFKHEDSQWSAPVDVKDNEELRKFPNVARDMARWGAVQFNTYQPGDTLMDLLT
                     AARGGFNRYLAEDVPQSAFDELARTIHDLVEMGYGWDPYPANILYDKQSRRFRLIDVR
                     KAGRFPAGYGSQLFVSGIGPMLGKISRSQGDADMEKIMKKILSAVTGAGFDPKQVHER
                     VEGILKDIGLSSERAVSLRLYAQKMTAEQPRRIPVAIPARSLGSETPATATAKRFDDL
                     YKTDWKRPLENLVEQVREATAGVNRARMTLLDLGCGDGAGGSLELARMGFGRSIHVDA
                     SHTAVERFKGKLGMSSPHVELREADLREVLRGLGDSSVDVVHAHLSLQYFSWRDTVEL
                     FSEIRRVLRPGGVLVFKVRSLDADRKGFVRIVNAGERDFYQRPDGRLIRLFSEQDVWD
                     VLSLARLETVSAPIQVSVPGWDDRPWVATARKPVGRYPTTPGAIFSISALHVGKLTTL
                     LWRVAQETVRLGIPLDDLFAELGRDWPGQSEDFERLKKMLARKLTAATIAQLEKFGDV
                     LLVPVMAKLLSAYLGRKEIKAKDLSPEEAEIYEAVSAGFARAQRDLESVGINKMDVRI
                     HEGTELFHGPDEYMMAPFIVNPDGTAVFHVNRILIDALMWLDGKKRDEYIELLVQREI
                     TLQKYLKEGLSFDEAHDKILKNTGQLELIAYVELYQRTKLSERGKVLKHFDALKRVFG
                     EQRFQEYLPGILILARNTKNLDFLFLHTLPELRWRSDDLWPSLVRAGRVADPVYLFTP
                     GLRARVANPQTVKEGETWAELAKVAASAGENSTVLLQMLESLVSMEPSILGENPFKKV
                     IHSPDDLLAAAKSLADALNKYDSISLRKFLAKIRFEFVWASVPAGLLSSREDVQKSIE
                     FTLNILSARANNPVSALGADEWRRKSSDFLRQLYAIANNSDDLSRIAEEAVKLQATSF
                     GKKLMAEHDGRIVMGLIRSPDDLGKLAASAPMLDEACELWGFETLVGWIRPLNDWHNP
                     STRSIDIVTTLDRWLARAARYNKLHRAWGDTRKDRRTLLQIQSLFLRKVADVDSEVDG
                     LFENAAELKRYFRDEYIASLFLKVISKSADIKERHDDVLRLCNAWKKTGVPLSELWPI
                     WEKLVSPQNAKEQIRYLEGNVGAVSLVAKNVFEQDLFVPDYQHHNYFTALVDYDVSGN
                     ADLLTLRSHLLAKKYAEARDILGRSANAAALALRAISENHFNDIISQTSDLILENTHH
                     GLMKNMKLGNMLWLTGDPLSDNNSLVALAKKRPLSPWTLDSSIEMLEHLGVRLTRNEH
                     REILSSLGFRTYKVRGNEVIVPTVFNTFTSAYRGSAEVDNVKASYLTYAMTTAFLHYA
                     NKGGENAKNGETSERVTGVNALHEFSEPALYYEGRTGERRVDSSVSLAFSYPEENGIL
                     PDYARKRVYRLDGGHLPHYVIQDLLKQQYAERRAKQNVFKVFRMIAEGKLSRTGLGRA
                     ENFVRAELKRLRGEAKLSEEYMEYLFGG"
BASE COUNT         1920 a         2417 c         2851 g         2073 t
ORIGIN      
        1 ccgacggcct gttcctccac cccctggtgg gggccaccaa ggaggatgac gtgccggcgg
       61 cggtgcgtat gcgatgctac gagacgcttt tgaaaaatta ttttcccgcc ggacggaccc
      121 tgctggcggt gaacccggcg aacatgcatt acgccggccc ccgggaggcg ctgttccacg
      181 cgttggtgcg gaagaactac ggttgcacgc attttatcgt cggaagggac cacgccgggg
      241 tggggaatta ttacggcccg tacgatgcgc agaaaatatt ttcaaatttc gctccggacg
      301 agttggacat cacgccgctg tttttcgaca acgccttctg gtgccgccgc tgtgcgggca
      361 tgtcgtcgga aaagacctgc ccccatgcgc cggaagaccg gctctttttg tcgggaagcc
      421 gtctccggga aatgcttcag cggggggaac ggccgcccgc cgaattctcc cgccccgaag
      481 tcatcgacat tttattggag agtttttcaa aacaaacccg ctcctgagtt ccccgccgtt
      541 tcgcccgccc ctctctcgcc catcgatcca tgaacccaga gggatttctg gggaagatgt
      601 tacatatccg ttacaactct ctcgctaaaa attaaattca aacgctttaa atatagctag
      661 ttacaaatag tctaagacac aaattacatt atagatgcct aaaccacgtc ttttctttcc
      721 ggtttcattc gtcagggcga caagtctcgt caccctgatt gtttttgttt tttccaccct
      781 cgccgtccct tacagtgaag cttcgttctg gtcggaacgc cgccgggccg ccgaaaatat
      841 cgtcaaagag tcggactctc cctccgccgt gttggcgcaa ttgccgcggg tggcctcgag
      901 ggatctttcc gctctttccg cgctgccgcc ggccctcaaa tccgtcgtcg atgcctcatc
      961 ggtgggggac tcggacctgc ctccgccggc cgccgccccg tggatgcgga gtctgttgtc
     1021 gtcttacgga gaggtccgtg acgctttccg ggctccgcag gagaaatcct gggtggtgtt
     1081 gctcgaggat cttcacacca gcaccgaggc gcaggagaac atctcgggcc tcatccgccg
     1141 gatcgccgat gaattcccgg cctcctcccc gtcgacggaa ggactgacga tcggcgtcga
     1201 aggcgcctgg ggggacttcg atctcgccgg tcaccggctt ttcgcggatc cggaggcgcg
     1261 ccgcccggtc tgcgacactc tgctgaattc cggcgttttg accggcgccg aacacgccgc
     1321 gctgacggtg gagcaccccg tgagactgct cggattggaa gaccccgctc tttacaggga
     1381 aaacatccaa gcctataagg attcccgcgc gacagcccct gcctttgaag caatttcgaa
     1441 acaatacgga cgccttctcg atcagaccaa gaaaaaccat tattccgccg cgcttttggg
     1501 attcgatggg gaggcgatcg cgtattcgcg cggaagccgt tctctcggtc agttcatcga
     1561 aacgatgcgg ggaacggtct tggacgacgc ctggatgcgg cgttatccca cggcggccgg
     1621 gttccggcag gttttggcgt tggagaaatc cttggatttt cagcgggtgg aacgggaacg
     1681 gaacgaaatc atttccggcc tcgtcgaatc cttgagcgac aaggaattgg cggagttcac
     1741 cgcttccgcc ttggcctatc ggctgggcga aatcacctac accgcgttct acacgggact
     1801 gcgacggttg ggcgaggcgc acggtgtctc atggacggac taccccgctc tggacgacta
     1861 ctttcgttat atctatctcg ccgcccggct ggacagggac cggctgtatc gggaagtgga
     1921 tgagtggcgc gacgagcttt atggccgtct gctgacgacc cccgaagaaa aacggataca
     1981 ttccctctcc gaggacttcg tcctcgtcac aaaactggcg gctttcagcc tgaactccgc
     2041 cgaatggggg cgggcgtgtc agcggcatga cgtgttcgct tcccttccga aacgtttcga
     2101 ggagtggggc ggctcctcga cggatgcgtc ggcatgggaa aaacagctgg aatccttcga
     2161 gaagttttac cggattgccg agaggcgcaa cgccgcgttg gtggacacac tggcgcgttc
     2221 ccagcggcgg ggtccgccgg cggtgcaact gatggtcctg ggagggtttc attcggaagc
     2281 ggtgaaggag atcctgcggg ggcgggggat gtcgtgggtg gtgatccgtc cgaacgtcac
     2341 gcgggccggc gatgcgccgc attacatgga tctcttcgcc cgagcccgga cgccgctgga
     2401 aaaacttttt gccggtgacg ccctcttcct gcgcagcccc ttggcccttt ctcgtgaggt
     2461 ccccaccggc tatgcccatt tgcgggtgac ggaacaaggg ctttatgtgt cgttgtccac
     2521 cgccaagacg cttctgaaga ccctgcgcga gatgggcgcg tccacccctt cacaacgccg
     2581 cgtcgcattg cagtcggtcg tggcacgttt gcaggaggcc gcccgccggt tcgatgccct
     2641 gggcgataaa ctggttttac gtttggaggg gaccgagggc gggacactga ccttttctgt
     2701 ccaatacgaa ggtcctcggc tcgaacaacg tcaatccgtg gtcctccgtg ttcaggcggg
     2761 ggggacggaa cctgccggag ccctgaagat gaccgtggga gaggatcctg tctttttgac
     2821 gccgacgaaa ggacttcgac aggtctttca gcggggaatg cgggaggtca agagtttttg
     2881 gaaccgcatc gtccggccgg cgttccgttt aaaacagctg ggcccctatc cgctgaagga
     2941 aaaaattcac caccaggttt atgtcgggca ggacccgacg acacttgagc cggtcgttct
     3001 gaaggcgccg gataagaaaa tacccggttt ccagcaggcg ccggccgaaa ccctcctcgg
     3061 gcgggaagcg cgtgtgctgg atttcataca gaaaaatgcg cctccggagg agcgctcctc
     3121 cttcccgaag ctcctcacgg acggggtccg gatcgatgaa ccgacccggt tgccgtatat
     3181 cgccatggag ttcgtggacg gccagacatt ggacgatgtt gtcaccaaag gacaaatcga
     3241 gccggaagaa cggttgggct tggccgccga gattctaagg gcgttggcga ttctccattc
     3301 catcggcgtg gtccacaacg atttcaagcc gacgaacgtc atggtgactc ggaacggcga
     3361 accacggatc aagctgatcg actttgaaat ttccgcggtg aaggggcttt cgttggaggg
     3421 tgacgccatg gccgcccaaa tcggggccat tccctacatg gccccggaac gcctgggacg
     3481 ggggccgtcc acttcggaag cggatgtgta tgccgccggc gtggtgctct atgagctgtt
     3541 gaccggacga cttcccttcg atttctggct ggcggaaagc gaagacgact actcattctg
     3601 gctggattgg atgatgaagg agcgggcgaa aacgccgaag ttcctggagg acgacgaccc
     3661 ccgctttcct ccggagataa aagcgcttct gaaacggatg ctggctatgg atccccaaga
     3721 ccgtccaacc gcatcggaag ctgtttcact tatggagggc ctgtcggtga cggccaggaa
     3781 aagcatgccc ccgccggccg cttttgcgga gacggccggt cgacgcggcg ccaccctgcg
     3841 ctggctgatg ccgctgggga tgtggctggc caagaaaata ggggtcgagt tcaaggagtt
     3901 gcccgccggc gcggattacc ggactctgtc gcccgaatcc cgcgcccggt tcgataagga
     3961 atacggcgcc aaagtcgcgt gggcgctgga ggaaacggcc gtctcccttt tgaccgtggc
     4021 gttgcagttc ctgcccggct tttccatgca tcttgtgctt gcctacgcgt cggtgaggat
     4081 cctcttcgtt ctgctgcatg tgccggactg gaccggcccg ccgaccctca aaaactttta
     4141 tgaccagttg ggcgccccgc tcatcgtttc ccttgtcccc gcggctgctt tcggtttggc
     4201 ccatgtcgtc tccctccccg tgtccctcgt ttttgcgttc aatatccttc ttggatttct
     4261 ggcccatcgt gtcgtcaacc tgggggttta cgaggggtgg agggcttttc ctgcccgccc
     4321 ggcaagtctc ttctcgttcg ggtctagaac ggcttttgag ttggaaatgg acgcggcccg
     4381 cctctcggcc cgcaagatcc cttcccgggt gctggaactt atccaaacca gtgagccgat
     4441 cggccgaggg gcggaggcgt gtgtttatcg gattccggag atgccgagca tcgtggcgcg
     4501 tgtccccgtg caaatggcgg atgaaatgcg gggcgctcca ttcgggcatc tttgggccaa
     4561 gcgattgctg gaagaaatgc gccaggggag gaaatgcgac gattttgtga agaacgcggc
     4621 cggcgccgcc atcgacgaaa aaaatgtccg accccttttg aacggaatca tcgcctcgcg
     4681 cgacttcaca tcttccgtcg atctggacct catccggttc gatgacttcg tggggccgct
     4741 tgcccgcaaa tacaagtccg gcgtccgatt gacggattcg gagcttcggg taatgaaccg
     4801 ctatcttctg gtgaaactgt atcccgtgac cctgaaagat tacatattca aacacgaaga
     4861 cagccaatgg tcggcgccgg tggacgtcaa ggacaacgag gagttgagga agtttccgaa
     4921 cgtcgcgcgc gacatggccc gttggggagc ggtgcagttc aatacttacc aacccggcga
     4981 cacccttatg gatctcctga cggccgcccg ggggggattt aatcgctatc tggccgaaga
     5041 cgttccacag tccgctttcg atgaattggc tcgcacgatc cacgatttgg tggaaatggg
     5101 ctacggctgg gatccgtatc cggcgaatat cctgtacgac aagcagagcc ggcgtttccg
     5161 gttgatcgat gtgcggaaag cggggcggtt cccggcggga tatgggagcc agctgttcgt
     5221 cagcgggatc ggccccatgt tggggaagat ttcaaggtcg cagggcgatg cggatatgga
     5281 aaagatcatg aaaaagatcc tctcggctgt gacgggggcg ggtttcgatc cgaaacaggt
     5341 gcatgaacgc gtggagggga ttctgaagga catcgggctg agttcggaac gggccgtgtc
     5401 gctgagactt tatgcgcaga agatgacggc ggaacagcct cggaggatcc cggtggccat
     5461 ccctgctcgt tccttgggat cggagacgcc ggctaccgcc acggcaaaaa gattcgacga
     5521 tctctataaa acggattgga agaggcccct cgagaacctc gtggaacagg tccgggaggc
     5581 gaccgccggg gtaaaccgcg cccgaatgac gttgctcgat ctgggatgtg gcgacggggc
     5641 gggggggtct ctggagttgg cgcggatggg tttcgggcga agcattcacg tggatgcttc
     5701 gcataccgcc gtggagaggt tcaaaggcaa gttggggatg tcgtctcccc atgttgagtt
     5761 gcgagaggcc gatctccggg aagttttgcg cggtctcggc gattccagtg tcgatgtggt
     5821 gcatgcccat ctctccctcc agtatttttc gtggagagac accgtcgagc ttttcagcga
     5881 gatccgccgg gtccttcggc cgggcggcgt cctcgtcttc aaggtgcgtt ccttggatgc
     5941 cgatcggaaa ggttttgtga gaatagtgaa tgcgggggag agggatttct atcagcgtcc
     6001 ggacggccgt ctcatccggc tgttttcgga acaggacgtg tgggatgttt tgtcgttggc
     6061 ccggctggag acggtatccg cgcccatcca ggtctcggtg ccgggatggg atgaccggcc
     6121 ttgggtggcg accgcacgaa aacccgtggg gcgatatccg acgacccccg gggccatctt
     6181 ctcgataagc gctcttcatg tggggaaact gacgactctg ttgtggcggg tggcccagga
     6241 aaccgttcgg ctggggattc cgctggatga tctgtttgcc gagctggggc gggattggcc
     6301 cggacaatcg gaagattttg agcggctgaa gaaaatgttg gcgagaaaat tgacagcggc
     6361 gacgatcgca cagctggaaa agttcggcga tgtcctgctt gttccggtga tggcgaagtt
     6421 gttgagcgcc tatctcggcc ggaaggaaat caaagcaaag gatttgagtc ccgaagaggc
     6481 tgaaatttat gaggcggtgt cggccggttt tgccagggcg caaagagatt tagagtccgt
     6541 cggcattaat aaaatggatg tgcgaatcca cgaggggacg gaacttttcc acgggccgga
     6601 tgaatatatg atggcgccgt tcatcgtgaa cccggacggg acggctgttt tccatgtcaa
     6661 taggattttg atagacgctt taatgtggct cgacgggaag aagcgggatg aatatatcga
     6721 gctgttggtc cagcgggaaa tcaccctcca gaaatacctg aaagagggcc tttctttcga
     6781 cgaggcgcac gataaaatat taaaaaacac cggacaattg gaattgatcg cgtatgtcga
     6841 gttgtatcaa aggaccaaat tgtccgaacg ggggaaggtg ctgaaacatt ttgacgcgct
     6901 aaagcgcgtc tttggtgaac aacgttttca ggaatatttg ccgggaatcc tgattttggc
     6961 cagaaatact aaaaatctgg actttttgtt cttacatacc ttgcctgaat tacgatggag
     7021 gtcggatgat ctttggcctt ctttggtccg cgccggcagg gtggcggatc cggtttatct
     7081 ctttacgccc ggcttgcgtg cccgagttgc caatccccaa accgttaagg agggagagac
     7141 gtgggctgaa ttggccaaag tggccgcttc cgcgggggag aattcgactg ttttgttgca
     7201 aatgttggag tccctggttt caatggagcc cagtatcctt ggggaaaatc cattcaaaaa
     7261 agtgattcac tcgccggatg atcttttggc ggcggcgaaa agccttgcgg atgccctgaa
     7321 taaatacgat tcgatttcgt tgcgaaaatt tttggcaaaa atacggttcg aatttgtttg
     7381 ggcaagcgtc ccggcggggc tcctatcgtc ccgggaggat gttcaaaaga gtatcgaatt
     7441 tacgttgaac attctgtccg ctcgtgccaa taacccggtt tcggcgttgg gggctgatga
     7501 gtggcgcagg aaatcctccg attttttgcg acagctttac gctatagcga acaacagcga
     7561 cgatttgtcc cggatagcag aagaagccgt aaagttgcag gcgacctctt tcggtaaaaa
     7621 actcatggcg gagcatgacg ggcgtattgt tatggggctg atacggtctc cggacgactt
     7681 ggggaaattg gcggcgtcgg cgcccatgct ggatgaggca tgtgaactgt gggggttcga
     7741 gaccttggtc ggatggatca ggccattgaa tgattggcat aatccatcga ctcgttcaat
     7801 agacattgtg acgacgttgg accgatggct ggctcgtgct gcgcgctaca acaaacttca
     7861 ccgggcgtgg ggggatactc gaaaagacag gcggacgctt ctgcagatcc agagcctatt
     7921 tctgaggaag gttgcggatg tcgattcgga ggtggacggt ctttttgaaa atgcggcgga
     7981 gttgaagcgt tattttcgag acgaatatat tgcctcgtta tttttaaaag tcatctcgaa
     8041 atcggccgac ataaaagaac gccacgacga cgttctgcga ttgtgcaatg cctggaaaaa
     8101 aacaggggtg ccgctctccg agctttggcc gatatgggaa aaacttgttt ctccgcagaa
     8161 cgccaaggaa caaatccgtt atttagaggg caatgtcggc gccgtttcgt tggtcgctaa
     8221 aaatgtattc gagcaggacc tgtttgttcc ggattaccag catcacaact attttacggc
     8281 cttggtggat tatgacgttt cgggcaatgc cgatctgctg acgcttcgaa gtcatttgct
     8341 ggctaaaaaa tatgccgaag ccagggatat tttgggccgg tccgcgaatg cggcggctct
     8401 cgcgcttcgg gcgatatcgg aaaatcattt taacgacatt atttcccaaa cgtctgattt
     8461 gatccttgaa aatacacatc acggactcat gaaaaatatg aagttgggca acatgctttg
     8521 gctgaccggc gatcctttgt ctgataacaa ttccctggtg gctttggcca aaaagaggcc
     8581 gctctcccct tggacgctgg actcaagcat tgagatgctt gaacatttgg gggtccggct
     8641 gacgagaaat gaacatcggg aaattttgtc gagtttggga ttccggacct ataaagtgag
     8701 gggaaatgaa gtgatagtgc ccacggtgtt caatacgttc accagcgcct atcgggggtc
     8761 ggcggaggtg gacaatgtca aggcgagtta tctgacctat gccatgacga cggcgttcct
     8821 gcattacgcc aacaagggtg gtgaaaacgc taaaaatggg gagacgtcgg agagggtcac
     8881 cggggtaaac gcgttacatg agttttcaga accggctttg tattatgagg ggcgtaccgg
     8941 tgaacgaaga gtggattcca gcgtttctct ggcattcagc tatcctgaag agaacggcat
     9001 cttgccggat tatgcccgta aacgggtgta tcgtcttgac ggaggacact tgccacatta
     9061 cgtcattcag gacctgctga aacaacaata cgcggagcgg cgggccaagc agaatgtatt
     9121 taaggtgttt cgcatgatag cagaagggaa attgagtcgg acgggacttg gaagagcgga
     9181 aaattttgtg cgcgcggaac tgaaacggtt gaggggcgaa gccaaactga gcgaagaata
     9241 tatggaatat ctgttcggtg g
//
LOCUS       DAOTFD010000087         9229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5316_length_9229_cov_3.927077, whole genome shotgun sequence.
ACCESSION   DAOTFD010000087 DAOTFD010000000
VERSION     DAOTFD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5316_length_9229_cov_3.927077"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3620)
                     /locus_tag="P5079_06380"
     CDS             complement(<1..3620)
                     /locus_tag="P5079_06380"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HRY29651.1"
                     /translation="MRRLVSCTMALVMLTENAAFLHSSEGNLWKQRRDALEKAAAKQT
                     LQSVPAVSNTASESQLYAQLAPSAAQTVVPEITAGLDQMAVSQALSSAQKTSLHGTEV
                     PRWLKSIVGAYGNVQEIRLPAQTRPSRYVVHLLDVHEAYEGQINMAQLMEQLAASTGD
                     KPSAPLRVGVEGASGGFDFQPYRHFASPAVVRSVSDLMVKEGLLSGAEFFGMTTQRPV
                     RLWGVEKKDSYLENVRAFKDSLASQSDDDATLREAAAAADKLKKQFFPPDLAELDRNM
                     TAFAQGQIGLGKYIRYLSAKADPSRMGTNLKVFLQALRLEEKLNLKQVEAERTKLIER
                     LVESLTKGNLEQLVKTSLAYRMGRSSYREYYGYLQDVCAAHAVPIKEYPQLDTYIQYV
                     LLAEKIQREPLLEELQAMENDVVYRSVASEAQRDLVAVSRDLRLAEKLNTFSLSNDDW
                     MKLDGRRQTIARLPVRLEKWGSVPSDLSEKWNSLIRHHEAFYRAAQKRNQDMVDNLLA
                     GRETDSSAVSLLVAGGFHSQGIRDLLLKKNIGYIALTPKLSQVKGLPHYLEPFSAEKT
                     PLDKLFAGERLFLKKALRGANEPLMGTPSDALQFTGFFAASSVAETIGEMLRERLGDA
                     LQPGETVALDLDDLAKMVGPDLAAAIREEFSNFEKAADEFPAPLAGLKLTPRITLVPY
                     ADRPGLREVSLKVSLERTGAEPLVFNIAYRFESIEALSGEEKAAKLSFGTDRRTFVWS
                     PATPSTLEEALAQLDALYDQARQWLTQKIAGFSSTAEEKANAETTFVAAAPDHRDAII
                     HDVAVFTAAGVVAEGHRGAVLGLTDKAEIARRKDAADGYAKRTAEAMFYARDARVFVH
                     VAEGIGRDEVQESFVGSEVINPKGLNEQHIIVDVIEGTNATASNNGGKSRAEIGAAQS
                     GGMMAIVSGSGIEALGNAPDVYADQLYSAVPPQYRAAVQALVEKNLEREDEAGLEEVL
                     QAIADVNNLTINDLEFVIMQRAREGNRMAALKAIQSRFPGMTLTQIGDGTVAHGLLAT
                     LGRKPGAGKHKVMWTVGGSAEAFMNLAVAAAVPTGVGALRVYSKKVNKTAAGEEAKNL
                     AQRYAFDEAEKKELRADRKDADAIIAGQKLFTVTDVAGTVDAEFAFITDNGVFNVPGV
                     QEIRPGVFRVHVLRIIEGKAAIVAPLPVLRSVLQLASENQLHDGDLVVLGVADGQLTE
                     MTT"
     gene            complement(3754..4407)
                     /gene="coaE"
                     /locus_tag="P5079_06385"
     CDS             complement(3754..4407)
                     /gene="coaE"
                     /locus_tag="P5079_06385"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRY29652.1"
                     /translation="MSPSSKRTKKTSLSGRRRLVVGLTGGIGSGKSTVLRMFRRLGCA
                     TADADAIAHRALEPGGPTYARVKRLFGPEIVGKDRQLNRARIADLVFRAPSLRRRLEH
                     LVHPIVLRELDRRIRRVKKGVLVLDIPLLFEGGYQSFVDTTAVVWCSRARRLRRLRRR
                     GKFSLPDIRRRMDSQIPLSQKRHRAAWVIDNNGALSQTRLTVERMVRLFKKLLKERL"
     gene            complement(4404..6533)
                     /locus_tag="P5079_06390"
     CDS             complement(4404..6533)
                     /locus_tag="P5079_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY29653.1"
                     /translation="MKAEWVQYEPVPAPPLFTNLRWGKVLMLPVGWAKAAYKWFTSGA
                     PGQVSPAKLLDYYNCPQKYKYVRVDKKPQRPSPHLSFYKALHGAIVEFHKTLQSNPTA
                     AANDLLAQYQSLWDPDGFANPEEEKLFYDRGLQALERYFALHHGKKPPDELRVPVEVE
                     ISGIPMKTTVDAVEKITPFSDFAADDLVPDAFKKELKAQRPVDGRMRTALGASADALE
                     ALSPDPLVSALNALLVSPDLHAHVDAGSLKVPRDIAPLLAEARALAADGKVPEETRLR
                     TLNKWLLWSSYRRGLRKTEHWRLTFFKTGQRVLSEASLTADLKSQMVYLMVRTKWPKV
                     RLDAQYYYLIADIRVAFKMKQEHMDDAREVIEEASRGIGSEEFEPKATHLCGWCDFQL
                     LCPHWHGYVTDRKRFRLSYSKMSCYLNCPRNYKFIYVDRVAPQPRNFFSIGTSLHNTM
                     EEFYMYDGIFKQPSFDYLVKLFVDNWLTAGYKDDADEKKFFESGMKQIRQYYISEVNG
                     KFRKAWAVEPFFELPVGEKAVMVGFQDRIQKNDDGTYEVLDYKTEPKVRTQAEVDEDL
                     QLTVYYWACLETFKITPARLTLLFTQFDQRVTTTRTPEHIPKLFDHVNKVVDTILNDK
                     EFAPRINKYCTGCDHLKGCPKEQEILEGAKAGKFLHKIDPQDVKPAPPAGGPLEPDEE
                     AEQEKQAEKNLSLLALEKDKEQPQPRG"
     gene            complement(6556..7335)
                     /locus_tag="P5079_06395"
     CDS             complement(6556..7335)
                     /locus_tag="P5079_06395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY29654.1"
                     /translation="MVINRPLSFRSLPPTIRGLIVLNGVVYLVQLFGGSRLVALLGLS
                     PARVLQDLWLWQLTTYMFLHGGFFHFLLNMYVLWAFGREIERQWGSWSFLWYYLLCGW
                     GAGVFNVLFSPTSPLPSIGSSGAIYGVLVAFAVMFPQSIIYLYFVIPFRAWQMVLMFA
                     AIEFLAGFSSAATGVANLAHLGGMATGYVYLKGSSFRWQVGRFLKNLRQTVTKKRADG
                     PALHDLDSEVDRILDKILKQGVNSLSREEQELMRRYSKMKK"
     gene            complement(7336..>9229)
                     /gene="polA"
                     /locus_tag="P5079_06400"
     CDS             complement(7336..>9229)
                     /gene="polA"
                     /locus_tag="P5079_06400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRY29655.1"
                     /translation="RFEFNSFLDEVAAQEPAARKKPLKVEILRTEKDLHAAVSTFRDS
                     KELGIGVVAIGPSALHAHIVGLALSAGPGAAYYLPVGHSYLGCPAQLPLSSVRDNLSE
                     VLANEKIHKTGHNLKFISLALHRHGVPFQGLGFDILVASYCLNPARVAQNLNALCRDL
                     LGEDLPALPAAEGKKGAKETPPDQMEIPILAEHAAAEAEAALRLRKKMGPLLREKDLH
                     ALYFDIELPLVSVLARMEETGIKIDVPYLKGLAREFAAEIAEHEKTIYTLAGETLNLN
                     SPKQLAVLLFEKLKLPVIRRTKTGYSTDEEVLQKLSALHPLPAKLVEYRELSKLKSTF
                     IDALLQLVDPADGRIHTSFNQAVTATGRLSSSDPNLQNIPVRTPHGRRIRQAFVADKG
                     GLLLSADYSQIDLRVLAHVSQDPVLCEAFRHGEDIHAATARELFGLKKGETPTEDQRR
                     VAKTVNFGIVYGQTGFGLAQQIGIPVAEAQRYIDRYFARYAGVHRWIEKILEQARRDG
                     HVTTLLHRIRYLPEIKAPNAAVRQFAERTAMNTPIQGTSADIIKVAMRDLAEWLQTEK
                     HRTRMLLQVHDELLFEVPREEFDAVAAPIRRKMEEAVRLDIPLVVDLKKGVNWFEMEK
                     IPAEKK"
BASE COUNT         1601 a         2672 c         3065 g         1891 t
ORIGIN      
        1 gtggtcattt ccgtgagctg gccgtccgcc acaccgagca ccaccagatc gccgtcgtgg
       61 agctggtttt cggaggcgag ctggagcacg gagcgcagga cgggcagggg agcgacgatg
      121 gccgccttgc cctcaatgat gcgcagcacg tgcacgcgga acacgccggg ccggatttcc
      181 tgcacgccgg gcacgttgaa cacgccgtta tccgtgatga aggcgaattc cgcatcgacc
      241 gtgcccgcga cgtcggtgac ggtgaagagc ttctggccgg cgatgatggc gtccgcgtct
      301 ttccggtcgg cgcgcagctc tttcttttcg gcctcgtcga aagcgtagcg ttgcgccaga
      361 tttttcgcct cttcgccggc ggccgtctta ttgacttttt tggaatacac tcgcagcgcg
      421 ccgacgcccg tgggcacggc cgcggccacc gccagattca tgaacgcctc ggcgctgccg
      481 cccaccgtcc acatcacctt gtgtttgccg gcgcccggct tgcggcccag ggtcgccaag
      541 agaccgtgag ccacggtgcc gtccccgatc tgcgtgaggg tcattccggg gaaacgggac
      601 tgaatggctt tcagggcggc catgcggttg ccttcgcggg cccgctgcat gatcacaaat
      661 tccaagtcgt tgatggtcag gttgttgacg tcggcgatgg cttggagaac ttcctccaag
      721 ccggcctcgt cctccctctc cagatttttc tccaccaagg cctgcaccgc ggcccggtat
      781 tgagggggca cggcgctgta aagttggtcc gcgtacacgt cgggcgcatt gcccagggcc
      841 tcgatgccgg aaccgctgac gatggccatc atgccgcccg actgggccgc cccgatctcc
      901 gcgcgcgatt tgccgccgtt gttgctggcg gtggcgttgg tgccttcgat cacgtccacg
      961 atgatgtgtt gctcgttcag gcctttcgga ttgatgacct cggaaccgac gaaactttcc
     1021 tgcacctcgt cccggccgat gccttcggcc acgtggacga agacccgggc gtcccgggcg
     1081 tagaacatgg cctccgccgt gcgtttggcg taaccgtcgg cggcgtcttt ccgccgggcg
     1141 atctcggcct tgtccgtgag ccccagaacg gcgccgcggt ggccttccgc caccacgccg
     1201 gccgcggtga aaacggccac gtcgtggatg atggcgtcgc gatggtccgg cgcggcggcg
     1261 acgaaggtcg tttcggcgtt ggctttctcc tccgcggtgg acgagaaacc ggcgatcttc
     1321 tgggtcagcc actgtcgggc ctggtcgtac agggcgtcca gttgggccag ggcctcctcc
     1381 agggtgctgg gggtggccgg ggaccacacg aacgtccgcc ggtcggtccc gaaggaaagt
     1441 ttggcggctt tctcctcgcc ggaaagcgcc tcgatggatt caaaacgata ggcgatattg
     1501 aagacgaggg gttccgcgcc ggtccgttcc aggctcacct tgagggagac ttcccgcaac
     1561 cccggccggt ccgcgtaagg gacgagggtg atccggggcg tcaacttcaa accggcgagc
     1621 ggcgcgggaa attcgtcggc cgctttttca aaattggaaa actcctcccg gatggcggcg
     1681 gccaggtccg ggccgaccat cttggcgagg tcatccaggt ccaacgcgac ggtttctccc
     1741 ggttgaaggg cgtcccccaa ccgttcccga agcatctccc cgatggtctc ggcgaccgag
     1801 gaggccgcaa agaacccggt gaattgcaaa gcgtccgagg gggttcccat gaggggctcg
     1861 ttcgcgcccc gcaaggcttt cttgaggaaa aggcgttcgc cggcgaagag tttgtcgagc
     1921 ggggtcttct cggcgctgaa cggttcgagg tagtggggca gtcctttcac ctgggaaagt
     1981 ttcggagtga gagcgatgta tccgatgttc tttttgagca ggaggtcgcg gattccctgg
     2041 gagtggaatc cccccgccac caggagggag accgccgagg aatccgtttc gcggcccgcc
     2101 aaaaggttgt ccaccatgtc ctggttccgt ttctgggcgg cccgatagaa ggcctcatgg
     2161 tgacggatca gggagttcca tttctccgac aagtccgacg ggacggagcc ccatttttcc
     2221 aggcggaccg gcagacgggc gatggtttgc cggcggccgt ccagtttcat ccagtcgtcg
     2281 ttggaaaggg agaaggtgtt cagcttctcg gccagacgga ggtcccggga cacggcgacc
     2341 aggtcccgct gggcctcgct ggcgacggag cggtaaacga cgtcgttctc catggcctgg
     2401 agttcttcca gcagcggttc ccgctggatc ttttccgcca gtagcacata ttggatgtac
     2461 gtatctaatt gaggatattc ctttatagga acggcatggg ccgcgcagac gtcctgcaga
     2521 tagccgtaat attcccggta agaggagcgc cccatgcggt aggcgaggct ggttttgacg
     2581 agttgttcca gattcccctt ggtgagggac tccaccagcc gttcgatgag tttggtgcgt
     2641 tccgcctcca cctgtttgag gttgagtttt tcctccagcc gcagggcctg caggaacacc
     2701 ttgaggttcg ttcccatgcg ggagggatcg gctttggcgg aaagatatcg gatgtatttg
     2761 cccaggccga tctgcccctg ggcgaaggcg gtcatgttcc ggtccagttc cgcaaggtcc
     2821 ggcgggaaga attgtttttt cagtttatcg gcggcggcgg ccgcttcccg aagggtcgcg
     2881 tcgtcatcgg actgggaagc cagggaatcc ttaaaagcgc ggacgttctc cagataggaa
     2941 tccttctttt ccacgcccca gagccggacg gggcgctggg tggtcatgcc gaagaattcc
     3001 gcacccgaca ggagcccctc tttcaccatg aggtcggaca ccgaccggac cacggcggga
     3061 gaggcgaaat ggcgataggg ttggaaatcg aaaccgccgg acgccccttc caccccgacc
     3121 cggagcggtg cggagggctt gtcgccggtg ctggccgcga gttgttccat gagttgggcc
     3181 atgttgatct ggccttcgta ggcctcgtgc acgtcgagca ggtggacgac gtagcggctg
     3241 gggcgggtct gggccggcag gcggatctcc tgcacgttgc cgtaggcccc gacgatggat
     3301 ttcaaccagc ggggcacctc cgtgccgtgg agcgaagttt tctgggcgga agaaagcgct
     3361 tgagaaaccg ccatctggtc cagtccggcg gtgatctccg ggacgaccgt ttgggcggcg
     3421 gaaggcgcca gttgcgcgta aagttgggat tccgaggcgg tgttggaaac ggcgggaacg
     3481 gattgaaggg tctgtttggc ggcggctttt tcgagggcgt cgcggcgttg tttccagagg
     3541 ttgccttcgc tggagtgcag gaacgcggcg ttctcggtca acatcaccaa cgccattgta
     3601 caagacacaa ggcggcgcat gtcccccctc cgaaccttcc gttcaatcgc tttaattagc
     3661 gttgattggc gcattgttgt tattagtata ggtgtgttgc aaaaagttta ccggaaaaac
     3721 ttgtaaattt attgtaacgg atttgtgacg cgtttacaac cgttctttta agagtttttt
     3781 gaagagtcgg accatccgtt cgacggtaag gcgcgtttgg gagagggcgc cgttgttgtc
     3841 gatgacccag gccgcgcgat ggcgtttttg cgagagcggt atttgggagt ccatccgtcg
     3901 gcggatatcg gggagggaga attttccccg ccgccgcaac cggcgcagac ggcgcgcgcg
     3961 ggaacaccac accaccgccg tggtgtccac gaacgattga tatcccccct cgaacagcaa
     4021 ggggatgtcc aggacgagga ctcccttctt cacgcgacgg atgcgccggt ccagttcacg
     4081 gaggacgatg ggatgcacga ggtgttccag ccgccgccgg agggacgggg cgcggaagac
     4141 gaggtcggcg atccgggccc ggttcagttg gcggtctttt ccgacgatct ccggaccgaa
     4201 gagccgtttc acccgcgcgt aggtcgggcc gccgggttca agggcccggt gggcgatggc
     4261 atccgcgtcg gcggtggcgc aaccgagccg acggaacatc cgaagcaccg tggatttgcc
     4321 ggaaccgatg ccgccggtga gccccaccac caggcgacgg cgccccgaga gggatgtttt
     4381 tttcgtgcgt ttcgacgagg ggctcatccg cgcggttgag gttgttcttt gtctttttcc
     4441 agcgccagaa ggctcaggtt cttctcggcc tgtttctcct gctcggcctc ctcgtcgggc
     4501 tccagcggcc cgccggccgg cggggcgggt ttgacgtcct ggggatcgat cttgtgcagg
     4561 aatttgccgg ctttggcccc ctcgaggatc tcctgttcct tgggacagcc cttaaggtgg
     4621 tcgcatccgg tgcagtattt gttgatcctc ggggcgaact ctttgtcgtt caggatggtg
     4681 tccaccacct tgttgacgtg gtcgaagagt ttcggaatgt gttccggcgt gcgcgtggtg
     4741 gtcacgcgct ggtcgaactg ggtgaagagg agcgtgaggc gcgccggggt gatcttgaac
     4801 gtctcgaggc aggcccaata atagacggtc agctggaggt cctcgtccac ctccgcctgg
     4861 gtccgcactt tgggttcggt cttgtagtcc agcacctcgt aggtgccgtc gtcgttcttc
     4921 tggatgcggt cctggaaccc caccatcacg gctttttcgc cgacgggcaa ctcgaagaaa
     4981 ggctccaccg cccaggcttt ccgaaacttg ccgttcacct cgctgatgta atattggcgg
     5041 atctgtttca ttcccgactc aaagaacttt ttctcgtccg cgtcgtcctt gtatccggcg
     5101 gtcagccagt tatcgacgaa cagtttcacc aggtaatcga aagacggctg tttgaagatg
     5161 ccgtcgtaca tgtagaactc ctccatggtg ttgtggagac tggtcccgat ggagaaaaaa
     5221 ttccgcggct ggggggccac gcggtccacg tagatgaact tgtagttgcg gggacagttg
     5281 aggtaacagc tcatcttgga ataggagagg cggaaacgct tccggtccgt cacgtacccg
     5341 tgccaatggg gacagaggag ctggaaatcg caccagccgc agagatgggt ggccttcggc
     5401 tcgaactcct cgctgccgat gccccgactc gcctcctcga tgacctctcg ggcgtcgtcc
     5461 atatgttcct gtttcatctt gaacgcgacc cgaatatccg cgatcagata gtaatactgc
     5521 gcgtccagcc ggaccttcgg ccatttcgtc cgcaccatga ggtagaccat ctgcgattta
     5581 aggtcggcgg tgaggctggc ctcggagagg acgcgctggc ccgtcttgaa aaaagtaagg
     5641 cgccaatgct cggtcttgcg caggccgcgc cggtaggaag accagagaag ccatttgttg
     5701 agcgtgcgga ggcgtgtctc ctccgggact tttccgtcgg ccgccagggc ccgggcctcc
     5761 gccaacaacg gggcgatgtc gcgggggact ttaagacttc ccgcgtccac gtgggcgtgc
     5821 aggtcgggcg agaccagcag cgcgttcagg gcggagacca gcgggtccgg ggagagggcc
     5881 tcgagggcgt ccgccgaagc ccccagcgcg gtccgcatcc gcccgtcgac ggggcgttgg
     5941 gccttcagtt cttttttgaa tgcgtccggg accaggtcgt ccgccgcgaa atcggagaaa
     6001 ggggtgatct tttcgacggc gtccacggtc gtcttcatcg ggattccgga aatctccacc
     6061 tccaccggca cccgcagttc gtccggcggc ttcttgccgt gatgcagggc gaagtaacgt
     6121 tccaacgcct gaaggccccg gtcatagaaa agtttctcct cctcgggatt ggcgaaaccg
     6181 tccgggtccc acagcgactg gtattgggcc agaagatcgt tcgcggcggc ggtgggattg
     6241 gactgcaggg tcttgtggaa ctccacgatg gcaccgtgaa gggccttata aaaggacagg
     6301 tgcgggctgg gccgttgggg ttttttgtcg acgcggacgt atttgtattt ctgcggacag
     6361 ttgtaataat ccagcagttt cgccggcgac acctggccgg gcgcgccgga ggtgaaccat
     6421 ttgtaggcgg ccttcgccca gccgacgggc aacatcaaga cttttcccca ccgcaggttc
     6481 gtgaagagcg gcggcgcggg gacgggttcg tattggaccc attcggcttt catggcgtct
     6541 ctccctcggc gaggttcact tcttcatctt cgaataacgg cgcatcagtt cctgctcttc
     6601 ccgagaaagg ctgttgaccc cctgcttgag gatcttgtcc aggatgcggt ccacctcgga
     6661 atccaggtcg tgcagggccg gtccgtcggc gcgttttttg gtaaccgtct gccggaggtt
     6721 cttcaagaaa cgcccgacct gccagcggaa ggaactcccc ttcagataaa cgtagcccgt
     6781 cgccatgccg cccaaatggg ccaggttggc cacgccggtg gcggcgctgg aaaaacccgc
     6841 caggaactcg atggcggcga acattaaaac catctgccag gcccggaacg ggatgacgaa
     6901 ataaagatag atgatgctct gcgggaacat gaccgcgaac gccaccagca cgccgtagat
     6961 ggccccggag gaaccgatgc tgggaagggg cgaggtggga ctgaacaaca cgttgaagac
     7021 gccggcgccc caaccgcaga ggagatagta ccacaggaaa gaccatgagc cccactggcg
     7081 ttcgatctcg cgtccgaagg cccacaacac gtacatgttc agaagaaaat ggaaaaaacc
     7141 accgtggagg aacatgtagg tggtcaactg ccacagccag aggtcctgca acacccgcgc
     7201 cggagacagg cccaacaggg cgacgagccg gctcccccca aagagctgga ccagatagac
     7261 gacgccgttg aggacgatga gcccgcggat cgtgggcggc agggaacgga acgagagggg
     7321 ccggttgatc accatctatt tcttctccgc cggtattttt tccatttcaa accagtttac
     7381 accctttttc aggtccacca caagggggat gtccaaccgg acggcttcct ccattttccg
     7441 tcggatgggc gcggccacgg cgtcgaactc ttcccgcggc acctcgaaca ggagttcgtc
     7501 gtgcacctgg aggagcatgc gggtgcggtg tttctccgtt tggagccatt ccgccaggtc
     7561 ccgcatggcc accttgatga tgtcggcgct ggtgccctgg atgggggtgt tcatggcggt
     7621 gcgttcggcg aactggcgca cggcggcgtt gggcgccttg atctccggca ggtagcggat
     7681 gcgatgcagg agggtggtga cgtggccgtc ccggcgggcc tgctccaata tcttttcgat
     7741 ccaccggtgg acgccggcgt agcgcgcgaa ataacggtcg atgtatctct gcgcctcggc
     7801 cacggggatg ccgatctgct gggccaggcc gaacccggtc tggccgtaga cgatgccgaa
     7861 gttgacggtt ttggccaccc gccgctggtc ttccgtcggc gtttccccct ttttgagccc
     7921 gaagagttcc cgggcggtgg cggcatggat gtcttcgccg tgccggaacg cctcgcagag
     7981 caccgggtcc tgggagacgt gcgctagcac gcggagatcg atctgcgaat aatcggcgga
     8041 gagaagaagg ccgcccttgt cggcgacgaa ggcctggcgt atccgccggc cgtgcggggt
     8101 gcggacggga atgttctgca gattggggtc gctgctggag agccgccccg tggcggtgac
     8161 ggcctggttg aagctggtat ggatgcggcc gtcggccggg tccaccagtt gaaggagcgc
     8221 gtcgatgaac gtggatttca gtttggagag ttcccggtat tccaccagtt tggcgggcag
     8281 agggtgcagc gcggagagtt tctgcagcac ctcctcgtcg gtggaatagc cggtcttggt
     8341 gcggcggatg accggcagtt tcaatttttc gaagaggagg acggccagtt gtttcgggga
     8401 gttgaggttg agcgtttcgc cggccagggt atagatggtc ttttcgtgtt ccgcgatctc
     8461 ggcggcgaac tcgcgcgcga gccctttgag gtacggcaca tcgatcttga tgccggtctc
     8521 ctccatgcgc gccagaacgg agaccagcgg cagttcgatg tcgaagtaaa gcgcgtgcag
     8581 gtccttttcc cggaggagcg gtcccatttt ttttcgcaga cggagggcgg cttcggcctc
     8641 ggcggcggcg tgttcggcca ggatcgggat ctccatctga tccggcgggg tttctttggc
     8701 cccctttttg ccttcggccg cgggaagggc gggcaggtct tcccccagga ggtcacggca
     8761 aagcgcgttg aggttctgcg ccacgcgggc cgggttgaga cagtaggagg cgacgaggat
     8821 gtcgaacccc agtccttgaa agggaacccc gtgccgatgg agcgccaggg aaatgaattt
     8881 cagattgtgg ccggttttgt ggattttttc gttcgccaga acctcggaca gattgtctcg
     8941 gacggacgaa agcggaagtt gggccgggca gccgagatag gaatggccca cgggcaaata
     9001 ataggccgcg ccggggccgg ccgacagagc caggccgacg atgtgggcgt ggagcgcgga
     9061 cgggccgatg gcgacgaccc cgatgcccag ttctttggag tcccgaaacg ttgagacggc
     9121 cgcgtggaga tctttttcgg tccggaggat ttcgaccttc agcggttttt tccgggcggc
     9181 gggttcctgc gccgccacct cgtccaggaa cgaattgaat tcgaaccgg
//
LOCUS       DAOTFD010000088         9140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5383_length_9140_cov_5.132746, whole genome shotgun sequence.
ACCESSION   DAOTFD010000088 DAOTFD010000000
VERSION     DAOTFD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5383_length_9140_cov_5.132746"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1699)
                     /locus_tag="P5079_06405"
     CDS             complement(302..1699)
                     /locus_tag="P5079_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29656.1"
                     /translation="MKKYLALLTVLAFTTLQSGWAGALGLKSISSPDGSIEISGQQAS
                     NETDFAPADDHRGSVVTRVRLGVNMDVTDDVRGRIEAVRACDPAGANVLYGNGKPETV
                     ANEEANIGIQNAYLDIDGLLGLDSFRVGRQYVGRPGDLIVYFGPLNDDILNVTALDSL
                     SITKKWWKLDITGVTGKIVDNTGVPGVTDTNADVAGDQNVSWITLNSDTLIPDMKVPL
                     EFGVYQYTNTGATAATNDNMTLAVYDLRAGYTLMDGAVNLGAEYAMNGGQRNGAGPGG
                     SNVDNKGTALLFNAMYDDKEVGWGAHLKYADASGDDPTETNSTSKDSSFRDIASDFRF
                     GEILSNSNRLATACPLGSGPGLDTGNFGCGLNVINIGGYYKLPVGNNKLTALADYYMV
                     SVNEVANSIDDGVGTEIDLTLQYAHSKDVTGAIGYATFSPDKGFINQYAANANLSDDP
                     VTKLFAKLMVKWGGQ"
     gene            complement(1956..2636)
                     /locus_tag="P5079_06410"
     CDS             complement(1956..2636)
                     /locus_tag="P5079_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29657.1"
                     /translation="MTNPVTPHRDAITQRIERGARWIFSHWQQVLLALAALAVAAALT
                     IFVIYNLRKLRTLAWEQLSSAQALAARGQRAEALQTIDQLLASTRTGPLVTQAHLFKG
                     DILLLEGKAADALQAYREADRQATTPALRALAAAGLGLAYEQNSQWTEAEDAYRRFIK
                     DFPEHFLTARTYESLGRIQTLQKKWGDAQGTFERLVTLYPTTLWARNAQDYLNYIKAN
                     LPAPPQNK"
     gene            complement(2664..2740)
                     /locus_tag="P5079_06415"
     tRNA            complement(2664..2740)
                     /locus_tag="P5079_06415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2704..2706),aa:Arg,seq:cct)
     gene            complement(2915..4612)
                     /locus_tag="P5079_06420"
     CDS             complement(2915..4612)
                     /locus_tag="P5079_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29658.1"
                     /translation="MTGLPPGGRPRPAFRKLALQRIFLALLGLTLSFALLEIGLRLGG
                     WGTLYFQNHPARTDIDDKKTYRILCLGESTTVVPSGASYPLQLEKILNDRDTGLRFIV
                     VNRGFAGATTSQLLDRLEYDLNKYRPDMCITMMGINDEETRPETERGHPVGDFIGSSR
                     TYKFVRFVGASLFARIRRSDWDRKIEMPPIEPSPEKTADRDALVETAYDHLRKEEFGR
                     AEKAFLQALQQAPDDPAALEGLGETYRLQEKFPEAENVFRKMLRQNPNDERALLALAK
                     NYAAQEKPADAQEAFREVLRRNPGQKGARLGLARAFRDQGRLSEAEKNIREVLRRDPA
                     DTNARVELAKNYQAQGRNAEAEKLFREIIGRNRDRKNFFPALLDFFKTETTFLETLIL
                     QPNNAAVHLGLARHYCGQGKFEAAEKVCRDFLRRDPLSVLPKRFLAVIYLQTGRLDMA
                     AELWEEINRRLDYPDVTKENYRKLRATLAARDIRYVCVQYPMRPIAPLKKIFESTAGI
                     TFVDNESVFKQAVQREGSSPIFSDQFAGDFGHATPRGNRLLAENVADAVLRALRPDQ"
     gene            complement(4609..6294)
                     /locus_tag="P5079_06425"
     CDS             complement(4609..6294)
                     /locus_tag="P5079_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29659.1"
                     /translation="MSPPTLPPNAPLPRSSPAPEPLGHAGRLIALLCVSAFLILSFWN
                     FGAPGLDDDEAVIGVTTGWIDLSPILHSFSFSHLPLMGYSNHAAMMCYWIFPFFKLLN
                     TPFLALRLSSLLFGLAALLLTFQLSRRLFGRSVALLTLGLLAVHPNFLIGSRLGAHHG
                     ILLCLFSTSSLLFFHRWWTDRRGSDFGLGFLCLGLGLSTFTYFLFWIGAIAATALLFA
                     KKLFPPFRARWKTLCAAGIVSFSVGAWPLLAAFHRQGMAEKVLEYISSSEQSQSRLTT
                     FAVFSPRKSFRVLGDLLTGADLRTAYWNDVSFWDFHARAASYLLALALAFFWISRRSL
                     FDKKHARPPASALGLLSVIFFDALLNSYQPSGKYGDHFLHLLPIVLLFTAAGLGDLFN
                     AALRPGTRALGLALAGGGLALSAMTVNVEYLFYLQKTGGVDFASGAVHDLADWVQVKG
                     YRSLVENEPPRLTMKLWFLTSKKFETQQFTGHALFGQAPRNYPSPAENFDALILDWKK
                     SGRSGHALSPSALKNLSSWRDARGRKLKLAAVFREKHGRPAYLVYEPRPPKQP"
     gene            complement(6345..6869)
                     /locus_tag="P5079_06430"
     CDS             complement(6345..6869)
                     /locus_tag="P5079_06430"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRY29660.1"
                     /translation="MPKKTDLKDFIRDIPDFPKKGILFKDVTPLLADAGAFQETVDRM
                     AAALKGLPVKKVVAIESRGFIFGAAVAQKSGLGFVPVRKKGKLPAKTLSVTYDLEYGT
                     DTLEMHADALAPGEKVAVVDDVLATGGTARAAAELVERAGAKPEILLFLIELKFLDGR
                     KKLDGRPMKAILDY"
     gene            complement(6905..7735)
                     /locus_tag="P5079_06435"
     CDS             complement(6905..7735)
                     /locus_tag="P5079_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY29661.1"
                     /translation="MPDSLDSVALYFRGIKRLPETDKEAMMVLWKRCKKGDKAAAKRL
                     VEANLRLVIPIAKKYYRTGVDFLDLIEEGNLGLMHAVEKFDPRRGFRFSTYAAYWIEQ
                     AVRRALDEQSKTIRIPPHALESLRKWMREWDRLHSALGRDPTLQEMADRLNLTARQLK
                     SVLDAHEASHSVGSLDTPLDDEENLFIRDIVSNKQEQLPDQILSMLRTNDELIKALEH
                     IGDRERRILDMRFGLSGKERMTLEEAGRKLRLSRERVRQLEERGLMRLKRVAQRMGLL
                     "
     gene            complement(7782..8063)
                     /locus_tag="P5079_06440"
     CDS             complement(7782..8063)
                     /locus_tag="P5079_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRY29662.1"
                     /translation="MSSVRRRYRIFGRVQGVGFRYFCLETARTLALRGWVRNCPDGSV
                     ETEAEGPAADLASFAERLQTAHSWARVERMETVEMSPRNDTTEDFEITG"
     gene            complement(8068..8748)
                     /locus_tag="P5079_06445"
     CDS             complement(8068..8748)
                     /locus_tag="P5079_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29663.1"
                     /translation="MRKSSLLIAVLGGTLMLFPPPGRADEAYNLDAQSLPGYTGHISA
                     PSAKTLSQHQSSIGLHRFLLGLAYGWPRDTEFGVMFELREVTPVTPFTRDTYRARAPY
                     FSFHAKHRFLREERHHVDAAVGVWRHTYYAVFSPGTWFGRYRLEGGPSLRRRTDDTHQ
                     IGSFVAFSRTTTFQRLFVDYDSQTELAGLGWRYLLSDAIRLDLLLTGLGRHEDFFDRF
                     IFGLSIAR"
     gene            complement(8757..8829)
                     /locus_tag="P5079_06450"
     tRNA            complement(8757..8829)
                     /locus_tag="P5079_06450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8794..8796),aa:Pro,seq:cgg)
     gene            complement(8864..>9140)
                     /locus_tag="P5079_06455"
     CDS             complement(8864..>9140)
                     /locus_tag="P5079_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY29664.1"
                     /translation="EGIPVPFCGRRARFPRGPFVFACRFGAPVIPGVVLKEKPGVYRA
                     VVGDPLWPSGRGPEAVKELLEKLALVLEKYISRYGEQWYRFEPVWAD"
BASE COUNT         1662 a         2432 c         3223 g         1823 t
ORIGIN      
        1 cggtcgttga ggatgatgag gcgacccgat ccttcgatgg cggtgccgcg gaggcggtaa
       61 tccagcccca tgttgagaga ggcgaactcc gcccggccgc cggacggcag gagtccgagg
      121 aggagcagga gcgcgctgcc gcagggaagg gtcttcagac gtgttttcat gggcggtggt
      181 attctagcat cgttggacga ggcgggcaag attggaaaag agaacccccc tctccggcgg
      241 atggccgaag aggggggtcg ttgaaacgtt ctttcgctct gagcgaaagc gggtcagccg
      301 attattggcc gccccacttc accatcagtt tggcgaagag tttggtcacc ggatcgtccg
      361 acaagtttgc gttggccgcg tactggttga tgaagccctt gtccggagag aaggtggcgt
      421 acccgatcgc tcccgtgacg tccttggaat gggcgtattg gagggtcagg tcgatttcgg
      481 tgcccacgcc gtcgtcgatg ctgttggcga cttcgttcac cgagaccatg tagtagtccg
      541 ccagggcggt gagcttgttg ttgcccacgg gcagcttata gtaaccgccg atgttgatga
      601 cgttcagacc gcaaccgaag ttgccggtgt cgagtccggg accggagccg agagggcagg
      661 cggtcgccag gcggttgctg ttgctcagga tctcgccgaa gcggaaatcc gaagcgatgt
      721 cgcggaacga gctgtccttg gaggtggagt tggtttccgt gggatcgtcg ccggaggcgt
      781 ccgcgtattt caagtgcgct ccccagccca cttccttatc gtcatacatg gcgttgaaga
      841 ggagggcggt tcccttgttg tcgacgttgc tgccgccggg gcccgcgccg ttcctctggc
      901 cgccgttcat ggcgtattcc gcgccgaggt tgacggcgcc gtccatgagg gtgtatcccg
      961 cgcgcagatc atagaccgcg agggtcatgt tgtcgttggt ggccgcggtg gcgccggtgt
     1021 tggtgtactg gtagaccccg aattccagcg gcactttcat gtcggggatg agggtgtccg
     1081 agttcaaggt gatccaggac acgttctggt ctccggccac gtccgcgttg gtgtcggtga
     1141 cgccgggcac gccggtgttg tccacgattt ttccggtgac gccggtgatg tccagtttcc
     1201 accatttttt ggtgatgctc aacgagtcga gcgccgtcac gttgaggatg tcgtcgttga
     1261 ggggcccgaa ataaacgatg aggtcccccg gacgtccgac gtattgacgg ccgacgcgga
     1321 agctgtccag gccgagcaag ccgtcgatgt ccaagtaggc gttctggatg ccgatgttgg
     1381 cttcttcgtt ggcgacggtc tccggctttc cgttgccgta gagcacgttg gctccggccg
     1441 gatcgcaggc gcgcaccgct tcgatgcgtc cgcggacatc gtcggtcacg tccatgttga
     1501 cgcccaagcg gacgcgcgtc accacggaac cgcgatggtc atcggccggt gcgaagtcgg
     1561 tttcgttgct ggcttgctgc ccgctgattt cgatggatcc atcgggcgag gagatgctct
     1621 tgagtcccag cgcgccggcc caaccggact gcagggtggt gaaggccaac acggttaaga
     1681 gtgccaagta tttcttcatt tcgggttatt cccccttttg atttttggga gcggacctct
     1741 gcggctaccc ccccgtctag gcgacagaaa gaaaaatgcc tctttctgcg ggaaatcccc
     1801 cccctgaggg aggaatttca gaataggctt agacgtccgc ccgtgttgct gtaaaaaaac
     1861 cgagttctga tggaacattt tccatcataa actcggcgtg attctctcat ctgcaaagaa
     1921 tattaacagg tgaaagtggg attgtcaaca aaaaattatt tattttgtgg cggtgccggc
     1981 aggttggctt ttatgtaatt gagataatcc tgggcgtttc gggcccagag cgtggtgggg
     2041 taaagggtga ccaggcgttc aaaggtgcct tgggcgtctc cccatttttt ttgaagggtc
     2101 tgtatccggc cgagggactc gtaggttcgg gcggtgagga aatgttccgg gaaatccttg
     2161 atgaaccggc gatatgcgtc ttccgcctcg gtccattggg aattctgctc gtacgccagt
     2221 ccgagcccgg cggccgccag ggctcgcagg gcgggcgtgg tggcctggcg gtcggcttcc
     2281 cggtaggctt gaagggcgtc ggcggctttc ccctccagga ggaggatgtc tcctttgaag
     2341 agatgggcct gggtcacgag ggggccggta cgggtggagg cgaggagttg gtcgatggtt
     2401 tgcagcgcct cggcgcgttg gccgcgggcg gccaaggctt gggcggagga gagctgttcc
     2461 caggcgaggg tgcgcagttt gcgcaggttg tagatcacga agatggtcag cgcggccgcc
     2521 acggccagcg cggccagggc cagcagtacc tgttgccagt gggagaagat ccaccgagcg
     2581 ccgcgttcga tgcgttgggt gatggcgtct ctgtgcggag tgacggggtt ggtcatcttg
     2641 tctgaaatcc tttcatcgaa atttggcgcc cccgggcgga attgaaccac caaccttctc
     2701 cttaggacgg agccgctcta tccaattgag ctacgggggc gaaatatcca tatcacgagc
     2761 tccctgtcgc tcgaccatcg aggttgtgac ggggacgtga cagccgttga cgcctcttcc
     2821 cgcttacccg gccgaggtgg cggaagagca aggtgagaat tgtagcagat ttggtttgaa
     2881 ccggagggcg gccggaggat cgccccgttg ggatctattg atcgggacgg agggcgcgaa
     2941 gaacggcgtc cgccacgttt tcggccagga gccgattgcc gcggggcgtg gcatggccga
     3001 aatccccggc gaactgatcg ctgaaaatgg ggctggaccc ttcccgttgg accgcctgct
     3061 tgaagaccga ctcgttgtcg acgaacgtga tccccgccgt cgactcgaat attttcttga
     3121 ggggcgcgat ggggcgcatg gggtattgaa cgcaaacgta tcggatgtcc cgcgcggcaa
     3181 gcgtggcgcg cagttttcgg taattttctt tcgtgacgtc cgggtagtcg aggcgccggt
     3241 tgatctcttc ccagagttca gcggccatgt cgagccggcc ggtctgcaaa taaatgacgg
     3301 caaggaatcg tttgggaagg acggaaaggg ggtcccttcg gaggaaatcc cggcagactt
     3361 tttcggcggc ctcgaatttg ccttgtccgc agtagtgccg ggccagtccg agatgcacgg
     3421 cggcgttgtt cggctgcagt attagggttt cgaggaaggt cgtctccgtt ttgaaaaaat
     3481 cgagcaaggc gggaaagaag ttttttcggt cgcggtttcg gccgatgatt tctcgaaaca
     3541 gtttctccgc ctcggcattc cgtccctgcg cttggtaatt cttggccagt tcgacgcggg
     3601 cgttcgtgtc cgccgggtcg cggcgaagga cttctcggat gtttttttcc gcctcggaaa
     3661 gcctgccctg gtcgcggaac gcgcgggcga gtccgaggcg cgcgcccttt tggccgggat
     3721 tccggcggag gacctctcgg aacgcctcct gtgcgtcggc cggtttttcc tgggcggcgt
     3781 aatttttagc cagcgccaaa agggcgcgtt cgtcgttcgg gttttgtcgg agcatttttc
     3841 gaaaaacgtt ttcggcttcg gggaattttt cctggaggcg gtaggtctct cccagcccct
     3901 ccagggcggc ggggtcgtcc ggggcctgtt ggagggcctg gaggaaggcc ttttccgccc
     3961 ggccgaattc ttcttttcgg agatggtcgt aagccgtttc gacgagggcg tcccggtcgg
     4021 cggttttttc gggagaaggt tctatcggag gcatctcgat ctttcggtcc cagtccgacc
     4081 gtcggatccg ggcgaagagg gaggcgccga cgaaccggac gaatttatag gttcgggacg
     4141 agccgatgaa atctccgacc ggatgtcccc tctccgtctc cggccgggtt tcctcgtcgt
     4201 tgatccccat catggtgatg cacatgtctg gccgatattt gttcaggtcg tattccaggc
     4261 ggtccaacag ttgggaggtg gtcgccccgg cgaacccacg gttaacgacg atgaaccgga
     4321 ggccggtgtc tcggtcgttc aatatttttt caagttgcag aggataggag gccccgctgg
     4381 ggaccaccgt ggtggattcc cccaggcaga ggatgcgata tgttttttta tcgtcgatgt
     4441 cggtccgggc gggatgattt tgaaaataga gggttcccca tccgcccagt cggagtccta
     4501 tctccaggag ggcgaaactc agggtgagcc ccaggagcgc caaaaaaatc ctttgcagcg
     4561 cgagtttgcg gaacgccggc cgagggcggc cgcccggagg aaggccggtc atggttgttt
     4621 cgggggccgg ggttcgtaga cgaggtaggc cggccggccg tgtttttccc ggaagacggc
     4681 ggccagcttc agtttccgtc cgcgggcgtc gcgccaggag ctgaggtttt tcaaggcgga
     4741 tggagagagg gcgtgtcccg agcgtccgga tttcttccag tccaggatga gggcgtcgaa
     4801 attttcggcg ggactggggt aattgcgggg cgcttggccg aagagggcgt ggccggtgaa
     4861 ttgttgggtc tcgaatttct tcgacgttaa aaaccacaac ttcatggtga gccggggcgg
     4921 ttcgttctcc acaagggagc ggtaaccctt tacctggacc caatccgcca ggtcgtggac
     4981 ggctcccgag gcgaaatcca cgcctccggt tttctgaagg tagaaaagat attcgacgtt
     5041 gacggtcatg gcggagaggg ccagtccgcc gccggcgagg gccaggccga gggcgcgggt
     5101 tccggggcgg agggcggcgt tgaacaaatc gcccaggccg gcggcggtga acaggaggac
     5161 gatggggagg agatggagga aatggtcgcc gtatttcccg gagggttgat aagaattaag
     5221 gagggcgtcg aaaaaaatga cggagaggag ccccagggcc gaggcgggcg gcctcgcgtg
     5281 ttttttatcg aagagggaac gccgggaaat ccagaagaag gcgagagcga gagcgaggag
     5341 gtaagaggcg gccctggcgt gaaaatccca gaaagagacg tcgttccaat aagccgtccg
     5401 caagtccgcg ccggtcaaga ggtcccccag gacccgaaag ctcttccggg gagaaaagac
     5461 ggcgaacgtc gtgaggcggg actgcgattg ttcgcttgag gaaatgtatt ccagcacttt
     5521 ttcagccatg ccttgacggt ggaacgcggc cagaaggggc caggcgccga cggaaaagga
     5581 gacgattccg gcggcacaga gggtcttcca tcgcgcccgg aacggcggga acagtttttt
     5641 tgcgaaaagg agggcggtgg cggcgatcgc cccgatccag aagaggaaat aggtgaaggt
     5701 ggacaggccc agtccgaggc agagaaatcc caggccgaag tcggacccgc ggcggtccgt
     5761 ccaccagcgg tggaaaaaga gaagagagga ggtggaaaaa aggcagagca gtatgccgtg
     5821 gtgggcgccc aggcgggacc cgatcagaaa attgggatgc acggcgagga ggccgagcgt
     5881 cagcagggcc acggaccggc cgaagagccg gcgcgagagc tggaaggtga ggagcagggc
     5941 ggccagtccg aacaggaggg aggaaaggcg gagggcgagg aagggggtgt tgaggagctt
     6001 gaagaacggg aatatccagt agcacatcat ggccgcgtgg ttggaatagc ccatcagggg
     6061 aagatgggag aacgagaagg aatggaggat cggggaaagg tcgatccagc cggtggtgac
     6121 cccgatcacc gcctcgtcgt cgtcgaggcc gggggcgccg aagttccaga aagagaggat
     6181 caggaaggcg ctgacgcaga gcagggcgat gagtcgcccg gcgtgaccca gaggttccgg
     6241 ggccggggag gaacggggga gcggggcgtt gggcggcagg gtcgggggtg acacaaaaga
     6301 ccgtggggac tcgccgatgg agcggatatc ccgcatggag gggatcagta gtcgaggatc
     6361 gctttcatgg ggcggccgtc gagttttttc cgaccgtcga ggaatttgag ttcgatgagg
     6421 aagaggagga tttccggttt ggcgccggcg cgttccacca gttcggccgc ggcccgggcg
     6481 gtgccgccgg tggcgaggac gtcgtccacc accgcgactt tttcgccggg ggcgagggcg
     6541 tcggcgtgca tttccagggt gtcggtgccg tattccaggt cgtaggtgac ggagagcgtt
     6601 ttggcgggca gtttcccctt tttgcggacg gggacgaacc cgaggcccga tttttgggcg
     6661 acggcggcgc cgaagatgaa gccgcgggac tcgatggcca ccactttttt tacggggagt
     6721 cctttcaggg ccgccgccat ccggtcgacg gtttcctgaa aagcgccggc gtcggcgagc
     6781 agcggggtga cgtctttgaa gaggatcccc ttcttgggaa aatcggggat gtcgcggatg
     6841 aaatccttga ggtctgtttt tttcggcatg ggggcgggcc ctttcggaat tttaggaaga
     6901 ggagtcagag gaggcccatg cgctgggcga cgcgtttgag ccgcatgagg ccgcgctctt
     6961 ccagctggcg gacgcgttcc cgggagaggc gcagtttgcg acccgcctcc tccagggtca
     7021 tgcgttcctt gccgctgagg ccgaaacgca tgtcgagtat ccgtcgttcg cggtcgccga
     7081 tgtgttcgag cgccttgatg agctcgtcgt tggtgcggag catggagagg atctggtcgg
     7141 ggagttgctc ctgtttgttg gagacgatgt ctcggatgaa aaggttctcc tcatcgtcga
     7201 gcggcgtgtc gaggctgccg acgctgtggg aggcctcgtg ggcgtccagg acgctcttga
     7261 gttgccgggc ggtgaggttg agccggtcgg ccatttcctg gagggtgggg tcgcggccga
     7321 gggcgctgtg gaggcggtcc cactcccgca tccatttgcg cagggattcc agggcgtgcg
     7381 gcgggatgcg gatggtcttg gattgttcgt cgagggcgcg gcgcaccgcc tgttcgatcc
     7441 aataggcggc gtaggtggag aagcggaatc cgcggcgggg gtcgaacttt tccaccgcgt
     7501 gcatgaggcc gaggttcccc tcctcgatga gatcgagaaa atccacgccg gtgcggtaat
     7561 attttttggc gatggggatg acgaggcgga ggttggcctc gaccagccgt ttggcggcag
     7621 ccttgtcgcc ctttttgcag cgtttccaaa gaaccatcat cgcttcctta tcggtttccg
     7681 gcaggcgttt gatgccgcgg aaatagaggg ctacggaatc gaggctgtcg ggcatggcga
     7741 gtgtgcagat tctccttctg aaaagggtcg gccggtgttc gtcagccggt gatctcgaaa
     7801 tcttcggtgg tgtcgttccg gggagacatc tccaccgttt ccatccgctc cacgcgggcc
     7861 cacgaatgcg ccgtttgcag ccgctcggcg aacgacgcca ggtcggccgc cgggccctcc
     7921 gcttccgtct ccacggagcc gtcggggcag ttcctgaccc agccgcgaag agccagggtc
     7981 cgggcggttt caagacagaa ataacggaac ccgacgccct ggacccgccc gaaaatccga
     8041 taccgccgcc gcaccgagga catgggctta acgtgcgatg gagaggccga agataaaacg
     8101 gtcaaaaaaa tcctcatgcc gtcccaggcc ggtcagcagg aggtcgagcc ggatggcgtc
     8161 ggagagcaga tagcgccagc cgaggcccgc gagttccgtc tgtgaatcgt aatcgacgaa
     8221 cagccgctgg aacgtggtgg tccggctgaa cgcgacgaac gagccgatct gatgcgtatc
     8281 gtcggtccgg cgtcggaggg acgggcctcc ctccaggcga taacggccga accaggtgcc
     8341 cggcgaaaaa acggcgtagt aggtatggcg ccacacgccg acggcggcgt ccacgtggtg
     8401 acgttcctcc cgcaggaacc ggtgtttggc gtggaaactg aagtagggcg cccgggcgcg
     8461 ataggtgtcc cgtgtgaacg gggtgaccgg cgtgacttcg cgcagttcga acatgacgcc
     8521 gaactccgtg tccctgggcc atccgtaggc caggccgagg aggaaacggt gcagtccgat
     8581 ggaggactga tgttgggaca aggtctttgc cgacggggcc gagatgtggc ccgtgtatcc
     8641 cggcaggctt tgggcgtcga ggttgtaggc ctcgtcggcc cggcccggcg gcgggaagag
     8701 catcagcgtt ccgcccaaga cggcgatgag taaagagctt ttccgcaccg gggatctcgg
     8761 ggtgcaggga tttgaaccct ggacctcttg gtcccgaacc aagcgcgcta ccagctgcgc
     8821 tacaccccgg gacccacggt gcggtgatgt tgcaaaatcg tttttaatcc gcccagaccg
     8881 gctcaaagcg ataccactgt tcgccgtagc gggagatata tttttcaaga acaagggcca
     8941 acttttcaag tagttccttg accgcctcgg ggccccggcc ggaaggccac aacgggtcgc
     9001 cgacgacggc gcggtagacg cctggttttt ccttcaggac cacgcccggg atgacggggg
     9061 cgccgaaccg gcaggcgaac acgaacggtc cccgcggaaa ccgcgcccgc cggccgcaaa
     9121 aggggaccgg gatcccttct
//
LOCUS       DAOTFD010000089         9071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5437_length_9071_cov_5.731921, whole genome shotgun sequence.
ACCESSION   DAOTFD010000089 DAOTFD010000000
VERSION     DAOTFD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_9071_cov_5.731921"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="P5079_06460"
     CDS             complement(<1..1113)
                     /locus_tag="P5079_06460"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRY29665.1"
                     /translation="MPLEKIDDFRWRIPKRGDMRVDGLIYADEKMLAHIVDEKAAEQV
                     ANVACLPGIVGRSLAMPDIHWGYGFPIGGVAATAVEDGVISPGGIGYDISCGVRLLGT
                     RLTLKEVERKLERLADGLFHNVPSGVGVGGKLRLSAQEIRKVFRKGARWAVENGFGDG
                     DDLDTCEDGGFLDTADPELPSERAVKRGLDQLGTLGSGNHFLEIQRVERVFDPVIANT
                     FGLFEGQIAVMVHTGSRGCGYQICEDSLAEMQTAVRKYGLSLPDRQLSCAPLSSPEGR
                     NYFAAMSAGANYARANRQVITHWVRETFVSILGASPRDLNMEVVYDVCHNIGKIEEHV
                     VDGQKRKLCVHRKGATRAFPAGRPEVDAIYGIPPTVA"
     gene            1258..2268
                     /locus_tag="P5079_06465"
     CDS             1258..2268
                     /locus_tag="P5079_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRY29666.1"
                     /translation="MSVNFFLAVVVILFTVGFNFVNGFHDAANVVATMISTRAFQPET
                     ALIIAATAEFIGPFIFGTAVAKTIGKDIISPEAITVTVILASLIGAIAWNLLTWYFGL
                     PSSSSHALVGGLGGAVLAGYGWHHLYLKGFLAIILVLITSPIIGLIAGYIFQKILIFF
                     SRGFSPKLNETYKKLQIVSALGLALSHGANDTHKSTGIITMTLVSLGILDSFHVPIWV
                     ILVCAPAMGLGTAFGGWKIIKTVGNKIYKLRPIHGFAAQAASGSVVLAAGLLGGPVST
                     THVMASSIAGVGSAERFKAVRWNTAYDIVIAWFITIPAAALMSALMYYLFSMISHFAK
                     GA"
     gene            2272..2886
                     /locus_tag="P5079_06470"
     CDS             2272..2886
                     /locus_tag="P5079_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRY29667.1"
                     /translation="MGLRDFFFPPKRDFVKMLQDQANKTCEGMKALYEYVQDPTKEKG
                     DQVVRLEEEADEMRRILVEELNKSFVTPFDREDIYSLSRTVDDMVDYAKSTVEEMDLF
                     GIKTNEHLKRMAEGLYNAAKDIAAAIPCLMKHPQTCGHHIVRAKKAENFVEHRYREAL
                     ADLFKSSDVVEILKCREIYRHLSNAADRGDEAANIIGDIMVKTT"
     gene            complement(2966..3388)
                     /locus_tag="P5079_06475"
     CDS             complement(2966..3388)
                     /locus_tag="P5079_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRY29668.1"
                     /translation="MPPPFEFFDHTADIGLIARGRDLPELFLNAARGLNALVLEPSAS
                     PPVTQKENIRLKSSDRDALLVQWLNELIFRLGVRRQVLWTARAFSVGDGVLVAAAEWE
                     TFDPARRRVLREVKSATYHELEIVEILGGWEARVVLDV"
     gene            complement(3470..5989)
                     /gene="gyrA"
                     /locus_tag="P5079_06480"
     CDS             complement(3470..5989)
                     /gene="gyrA"
                     /locus_tag="P5079_06480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY29669.1"
                     /translation="MADITLNKQGTLFDRIAPRNIEEEMKTSYINYAMSVIVGRALPD
                     VRDGLKPVHRRILYTMDEMGLAHNKPYKKSARVVGDVMGKYHPHGDTAIYDTVVRMAQ
                     EFSMRHVLVDGQGNFGSVDGDPAAAMRYTEVRLAAISQEMLGDIDKNTVDFGPNYDGS
                     LSEPLVLPAKLPNLLVNGSAGIAVGMATNIPPHNLSEVCDGAIAYIDNDQVTNADLYK
                     IMKGPDFPTAAIITGREGIKDYFETGRGSIHIRAKAEIEDIKGGKQAIIVDELPYQVN
                     KAQLLETIAELVRDKKIEDISDIRDESDRDGMRVVIEIKRDGNSQVVLNQLYKFTQME
                     TSFGVIMLALVDGRPRVLSMREMLHHYVEHRRTVVVRRTQYELKKAEDRAHILEGLRI
                     AIDNLNEIIKIIRQSKDVETARNALVERFKLSRVQAQAILDMRLHQLTALERKSLEDE
                     YLELIKTIARLKGLLADVRKIYGVIKEELKEIKEKYGEKRRTQITAAAADVEIEDLIT
                     QEDVVVTFSHAGYVKRLPIDTYKAQKRGGRGITGMTTKDEDFVEQLFVSNTHEYLQLF
                     TNRGRVYSIRVYEIPEAGRASRGKAAVNLVQLRPDEKITSAIPIKSFEEAKGKETALI
                     MCTRNGTVKKTVLSEFSDIRKSGIIAINLQEGDVLVDVKHGDDKCEVFIGTKDGMSIR
                     FPMEQIRPIGRSGMGVRGIRLAKGDQVVGMEVTTPGTRQTLITACENGYGKRTELSEY
                     RDQNRGGGGVITIKTSDRNGAVVGIKLVTNDNDIMFMTEKGMTVRVHAKDLSVIGRNT
                     QGYRLIRMEEGDKLASIAPVVVEDESDEDSSDSGAKSKDSK"
     gene            complement(6207..8750)
                     /gene="gyrB"
                     /locus_tag="P5079_06485"
     CDS             complement(6207..8750)
                     /gene="gyrB"
                     /locus_tag="P5079_06485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRY29670.1"
                     /translation="MGAKVEEKPVTRASGSSGDYDASKIQVLEGLEAVRRRPAMYIGS
                     TGAPGLHHLVYEVVDNSIDEVLAGYAKNVDVIIHEDNSITVQDDGRGVPVDPMKDVKD
                     PKLKNKSALEVVMTVLHAGGKFDHKAYKVSGGLHGVGVSCVNALSDWLDVEVYREGKK
                     YTQSYKRGKPTGDVKPLGKTDERGTRTTFHPDPEIFGDIKYSFETLANRLRELAFLNP
                     GTHINIQDERDDKQHAFCYEGGLMEFVKFMNAHKNPLHKDPIYLKKDKDDIIVEVALQ
                     YNDSYNEQIYSFVNNINTIEGGTHLSGFRSAMTRAINDYIKKHELTKGKDISITGDDT
                     REGLTAVISVKVPNPQFEGQTKTKLGNSEVEGLTKSIVGDILSTFFEENPNTASSICG
                     KIITASEAREAARKARELTRRKGALDGASLPGKLADCQERDPAKSEIFLVEGDSAGGS
                     AKQGRNRAFQAILPLKGKILNVEKSRLSKMLTNDEIRTMITAIGCGVGATAGEDGINL
                     DKLRYHKIVIMTDADVDGAHIRTLLLTFFFRQMRPLLENGFVYIAQPPLYKVKKGKKE
                     MYVETETEMDEWLLQEGLDSLEIATYIKGKPGPKLDTAKLKNAFKAITDVDILRKRLH
                     KKGVEWANFLEFKKKNAFPLYRVEDDNGVRFLYTDKEAKKWREEFVKNHKAKLQQELA
                     ASGEAATGSAGVEEEDISGYMKELLELKKMDALVVKLKEMGFDMLAEEPVKEEKPGKD
                     KPVPLYRAKIKDEEEKDLFSVNELFETIKGAGRQGATIQRYKGLGEMNPEQLWETTMN
                     PESRKMLQVKLEPNNSEAEDVFTILMGDKVEPRRQFIESHAAEVQNLDI"
     gene            complement(8775..>9071)
                     /locus_tag="P5079_06490"
     CDS             complement(8775..>9071)
                     /locus_tag="P5079_06490"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRY29671.1"
                     /translation="NYAVFEIWDRLLGVQATRAKAVGLQGRRLCVEVDSSARLHDLML
                     RKRTLLRKLNEHFGTRPVISDIILQLAQNPSVRRTMTFREKSGTRRPVHKKDPR"
BASE COUNT         1628 a         2702 c         2680 g         2061 t
ORIGIN      
        1 cgccacggtg ggcgggatgc cgtagatcgc gtcaacctcg ggccggccgg ccggaaaagc
       61 gcgcgtggcc cctttccgat gaacgcacag tttccgtttt tgcccgtcca cgacgtgttc
      121 ttcgatcttt ccgatgttat ggcaaacgtc gtaaacgacc tccatattca aatctcgcgg
      181 ggacgctccc aaaatggaga cgaatgtttc ccggacccag tgggtgatga cctgtcggtt
      241 ggcgcgggca tagttggctc ccgcggacat ggcggcgaaa tagttccgcc cctccgggga
      301 ggacagcggc gcgcaagaga gttgcctgtc cggcagggac aaaccgtatt ttcggaccgc
      361 ggtctgcatt tcggccaaag aatcctcgca tatctggtac ccgcacccgc gcgagccggt
      421 gtggaccatc accgcgatct gtccttcgaa aagtccgaac gtgttcgcga tcacgggatc
      481 gaagacccgc tccacccgct ggatttccaa aaaatgattg ccgctgccca gcgtgccgag
      541 ctggtcgagt ccgcgtttca cggcccgctc gctggggagt tccgggtcgg cggtatccaa
      601 aaaaccgccg tcctcgcaag tgtcaaggtc gtcaccgtcc ccgaaaccgt tctccaccgc
      661 ccaccgggcc ccttttcgga aaacctttct gatttcttgc gcgctcaagc gcagtttgcc
      721 gccgacgccc accccggacg gcacgttatg aaaaagcccg tccgccaagc gctccagttt
      781 cctttcgact tctttcagcg tcagtcgggt gcccaaaagg cggacgccgc aggagatgtc
      841 gtagccgatg ccgcccggcg agatcacgcc gtcctccacc gccgtggcgg cgaccccgcc
      901 gatggggaac ccgtatcccc agtggatgtc cggcatggcc agcgaacgtc cgacgatgcc
      961 cggcagacag gccacgttgg ccacctgctc ggcggccttc tcgtccacga tgtgcgcgag
     1021 cattttttcg tcggcataga tgaggccgtc cacacgcatg tcgccccgtt tcgggatccg
     1081 ccagcgaaaa tcgtctattt tttccaatgg catggccgcc ggagtcccac acccaagccg
     1141 atgtcggttt gtattgaaaa taccatatgt ttcatcgctc cttttcttga cagttggatt
     1201 tgagactact agaatctttc gctatcctaa ttcgcctcgc gggagcgttc cttggacctg
     1261 tccgtcaatt tttttctggc cgtcgtcgtc atccttttca ccgttggctt taatttcgtc
     1321 aacggtttcc atgacgccgc caacgtggtg gccaccatga tctccacgcg cgccttccag
     1381 ccggaaaccg ctctcatcat cgccgccacc gcggaattca tcggcccgtt catcttcggc
     1441 accgccgtgg caaaaaccat cgggaaagac atcatctccc cggaagccat caccgtcacg
     1501 gtcatattgg cctctctcat cggcgccatc gcgtggaacc tcctgacatg gtacttcgga
     1561 ttgccgtctt cgtccagtca cgccctggtc ggcggactgg gaggagccgt tttggccggg
     1621 tacggatggc accatctcta tctcaagggt tttcttgcca ttattctcgt cctcatcact
     1681 tcgccgatca tcggcttgat cgccggctac atttttcaaa aaatacttat tttcttttcc
     1741 cgcgggtttt caccgaaact gaacgagact tacaaaaaat tgcagatcgt ctccgccctt
     1801 ggattggcgc tctctcacgg tgccaacgac acgcacaaaa gcacgggcat catcacgatg
     1861 acgttggttt ctctcggcat actggacagc ttccatgtcc ccatctgggt catccttgtg
     1921 tgtgcgcccg caatgggtct tggaacggcg ttcggtggct ggaaaatcat aaaaaccgtc
     1981 gggaacaaaa tctacaagct ccgtcccatc catggcttcg ccgcgcaggc agcctcgggc
     2041 agcgtggtac tggccgccgg tctgttgggc ggccccgtct ccacaacgca cgtcatggct
     2101 tcttccatcg ccggcgtcgg cagcgcggaa cgtttcaagg ccgtccgttg gaacaccgct
     2161 tacgacattg tgatcgcctg gttcatcacc attccggccg cggcgctcat gtccgcgctg
     2221 atgtactacc ttttttccat gatttctcac ttcgccaagg gggcttaaga tatgggactt
     2281 cgcgattttt tctttccgcc gaaacgcgac ttcgtcaaaa tgcttcagga ccaggccaac
     2341 aaaacctgcg aagggatgaa ggcgctttac gagtacgtgc aggaccccac caaggaaaaa
     2401 ggcgaccagg tggtccggct ggaggaggag gcggacgaga tgcgccgcat cctggtggaa
     2461 gaactgaaca aatccttcgt gacgccgttc gaccgggaag acatttacag tctctcccgc
     2521 acggtggacg acatggtgga ctacgccaaa tccaccgtgg aagaaatgga ccttttcggc
     2581 atcaagacga acgaacacct caaacggatg gccgaaggcc tctacaacgc ggccaaagac
     2641 atcgcggccg ccatcccctg cctgatgaaa cacccccaga cctgcggaca ccacatcgtg
     2701 cgcgccaaga aagcggagaa ctttgtggaa caccgctatc gcgaggccct ggcggacctc
     2761 ttcaaaagtt cggacgtggt ggagatattg aaatgccgcg aaatctaccg ccacctttcc
     2821 aacgccgccg accgcggcga cgaggccgcc aacatcatcg gcgacatcat ggtgaaaacg
     2881 acctgacccg gaacgcgacc ttcttcgata aaaaaacagg cctctttcgg ggcctgtttt
     2941 tttatcgccg atccgaagtt ttaatctaca cgtccaacac cacccgcgcc tcccaccctc
     3001 ccaaaatctc cacgatctcc agttcatgat aggtggcgga tttcacttcc cgcagaacgc
     3061 gccgccgggc cgggtcaaag gtttcccatt cggccgccgc aaccaacacc ccgtcgccca
     3121 ctgaaaacgc ccgggcggtc cacagcacct ggcgccggac ccccaaccgg aagatgagtt
     3181 cattcaacca ttggaccaac agggcgtccc ggtcggagga tttgagacgg atgttttcct
     3241 tttgggtaac ggggggcgaa gccgatggct ccaaaacaag ggcgttcagc ccacgggcgg
     3301 cgttgaggaa aagttccgga aggtcgcggc cgcgggcaat caggccgatg tcggcggtgt
     3361 ggtcgaaaaa ttcaaacggc gggggcacgg gaggcgtcat gccgcggcaa agaaaaaacg
     3421 acagccggaa cgccggccgc ccccgagagg ccggagcgga cggccgaccc tatttcgaat
     3481 ccttcgactt tgccccggaa tccgaggaat cctcgtccga ttcgtcttcc accaccacgg
     3541 gggcgatgga ggcgagcttg tccccctcct ccatgcggat gaggcggtag ccctgcgtgt
     3601 ttcggccgat gacggacagg tccttggcgt gcacgcggac ggtcatgcct ttttcggtca
     3661 tgaacatgat gtcgttgtcg ttggtgacga gcttgatgcc caccacggcg ccgttgcggt
     3721 cggaggtctt gatggtgatg acgccgccgc cgccgcggtt ctgatcgcgg tattccgaaa
     3781 gttcggtgcg cttgccgtac ccgttctcgc aggcggtgat gagcgtctgg cgggtgcccg
     3841 gcgtggtgac ttccatgccc accacctggt cgcccttggc caggcggatg ccgcgcacac
     3901 ccataccgga acggccgatg ggccgtatct gttccatcgg aaagcggatg gacatgccgt
     3961 ccttggtgcc gatgaacacc tcgcacttat cgtcgccgtg tttcacgtcc accagcacgt
     4021 cgccttcctg caggttgatg gcgatgatgc cgctcttgcg gatgtcggag aactccgaca
     4081 ggacggtttt cttcaccgtg ccgttgcggg tgcacatgat gagcgcggtc tctttccctt
     4141 tcgcttcctc aaacgatttg atggggatgg cggaggtgat cttttcgtcc ggtcggagtt
     4201 ggaccaggtt caccgccgct ttgccgcggg aggcccggcc ggcttcgggg atttcataca
     4261 cccggatgga gtagacgcgt ccccggttgg tgaacaactg cagatattcg tgcgtattcg
     4321 agacgaacaa ctgttcgacg aaatcctcgt ctttcgtcgt catgccggtg atgccgcgcc
     4381 cgccgcgttt ctgcgcctta taggtgtcga tgggcaggcg tttcacgtag cccgcgtggg
     4441 agaaggtcac caccacgtct tcctgggtaa tgaggtcctc gatctccacg tcggcggcgg
     4501 cagcggtgat ctgcgtgcgc cgtttctcgc cgtatttctc cttgatctct ttcaattctt
     4561 ccttgatgac gccgtaaatt ttccgcacat ccgccagcag gcctttcagt cgggcgatgg
     4621 tcttgatcaa ttccagatat tcgtcttcca gggacttcct ctccagggcg gtcagttggt
     4681 gcaggcgcat gtcgaggatg gcctgggcct ggacccggga gagcttgaac cgttcgacca
     4741 gggcgttgcg ggcggtctcc acgtccttgg actgccggat gatcttgatg atctcgttga
     4801 ggttgtctat ggcgatccgc aagccctcca aaatatgggc gcggtcttcg gcttttttga
     4861 gttcgtactg cgtgcggcgc accacgaccg tgcggcggtg ctccacgtaa tggtgcagca
     4921 tctcgcgcat ggaaaggacc cggggacggc cgtccaccag cgccagcatg atgacgccga
     4981 aggaggtctc catctgggtg aacttataga gctggttgag caccacctgg gagttgccgt
     5041 cgcgcttgat ctcgatgacc acgcgcatgc cgtcgcggtc ggactcgtcg cggatgtcgg
     5101 agatgtcctc gattttctta tcgcgcacca gttcggcgat ggtctccaaa agctgggcct
     5161 tattgacctg atagggcaat tcgtccacga tgatcgcctg cttgccgccc ttgatgtcct
     5221 cgatttcggc cttggcgcgg atatggatgg atccgcggcc ggtctcgaaa tagtctttga
     5281 tgccttcccg gccggtgatg atggcggcgg tggggaaatc ggggcccttc atgatcttgt
     5341 agaggtcggc gttggtgact tggtcgttgt cgatataggc gatggcgccg tcgcacacct
     5401 cggagaggtt gtgcggcgga atgttggtgg ccatgcccac ggcgatgccg gcagagccgt
     5461 tgacgagcag attggggagt ttcgccggca aaaccagcgg ttcggagagg gagccgtcgt
     5521 agttgggccc gaaatccacg gtgttcttgt cgatgtcacc cagcatctcc tgggagatcg
     5581 cggccaggcg gacttccgtg taccgcatgg cggccgccgg gtcgccgtcc acggaaccga
     5641 agttgccctg cccgtccacc aggacgtggc gcatggagaa ctcctgcgcc atgcgcacga
     5701 ccgtatcgta gatggccgta tcgccgtggg gatggtattt acccatgacg tcgccgacca
     5761 cgcgggcgga cttcttatag ggtttgttgt gggcgaggcc catctcgtcc atggtgtaga
     5821 ggatgcggcg gtggacgggt ttcagtccgt cgcgcacgtc gggcagggcg cggcccacga
     5881 tgacgctcat ggcatagttg atgtaggagg tcttcatctc ctcctcgata ttgcgcggag
     5941 cgatgcggtc gaacagcgtg ccctgtttgt tcagagtgat gtcagccatt gtttctcccc
     6001 aaaactactg ttttaaaatt ttcatcgcgg agttataaaa acatgccgca ataacgggct
     6061 tgatacaaaa aataattaac ataccaacca ccacaaagat gccgaacccc gaccagaaca
     6121 aaattgcatt tctccgagtt gccttcccat cttgggcacg caagaaaagt cgccgggatg
     6181 aaggggcgcg aagccccttc aaaaaatcag atgtccaggt tctggacttc ggcggcgtgg
     6241 ctctcgatga actggcgccg gggctccacc ttgtcgccca tgaggatggt gaacacgtcc
     6301 tcggcctcgg aattgttggg ctccagtttg acctgcagca tcttgcgcga ttccgggttc
     6361 atggtggttt cccagagttg ttccgggttc atctccccca gacccttata gcgctggatg
     6421 gtggcgccct gccggcccgc ccccttgatg gtctcaaaaa gctcgttgac ggaaaacagg
     6481 tccttttcct cttcgtcctt gatcttggca cggtaaagcg gcacgggctt gtccttgccc
     6541 ggtttttcct ccttgaccgg ttcctccgcc agcatgtcga aacccatctc cttcagtttc
     6601 accaccagcg cgtccatctt tttcagttcc agcaactcct tcatgtatcc gctgatgtct
     6661 tcctcttcca cgccggcgct gccggtggcg gcctcgccgg aggcggcgag ttcctgctgg
     6721 agcttggcct tgtgattctt aacgaactcc tcgcgccatt tcttcgcttc cttgtccgtg
     6781 tagaggaacc ggacgccgtt gtcgtcttcc acacgataga gcgggaaagc gttcttcttc
     6841 ttgaactcca gaaaattcgc ccactccacg cctttcttgt gcaggcgttt gcgcaggatg
     6901 tccacgtcgg tgatggcctt gaaagcgttc ttgagtttgg cggtgtccag tttgggtccg
     6961 ggcttcccct tgatgtaggt ggcgatttcc agggagtcga ggccctcctg caggagccac
     7021 tcgtccatct ccgtttccgt ctcgacgtac atctcctttt ttcccttctt cactttatag
     7081 aggggcggct gggcgatgta gacgaagccg ttctccagga gcgggcgcat ctgccggaag
     7141 aaaaacgtca gcaggagcgt gcggatatgg gcgccgtcca cgtcggcgtc ggtcatgatg
     7201 acgatcttgt ggtaacggag tttgtcgagg ttaatgccgt cctcgccggc ggtggcgccg
     7261 acgccgcagc cgatggcggt gatcatggtg cggatctcat cgttggtgag catcttgctg
     7321 aggcgggact tctccacgtt cagaatcttt cccttgagcg gcaggatggc ctggaaggcc
     7381 cggttccgtc cctgtttggc ggacccgccg gcggagtcgc cttccaccag gaagatctcc
     7441 gatttcgccg ggtcgcgttc ctggcaatcg gcgagttttc cgggaaggga cgcgccgtcc
     7501 aaagcgccct tgcggcgggt gagttcgcgg gccttgcggg ccgcctcgcg cgcttcggag
     7561 gcggtgatga tcttgccgca gatggaggag gcggtgttgg ggttctcctc gaagaaggtg
     7621 ctgaggatgt cgcccacgat ggacttggtg agcccttcca cctcggagtt tccgagtttg
     7681 gtcttggtct ggccttcgaa ctgcgggttg ggcaccttca cggagatgac ggcggtgagt
     7741 ccctcgcggg tgtcgtcgcc ggtgatggaa atgtcctttc ccttggtcag ctcgtgtttc
     7801 ttgatgtaat cgttgatggc gcgggtcatg gcggagcgga accccgagag gtgagtgccc
     7861 ccctcgatgg tgttgatgtt gttgacgaag gaatagatct gttcgttgta gctgtcgttg
     7921 tactgcaggg cgacctcgac gatgatgtcg tccttgtcct tcttgaggta gatggggtct
     7981 ttgtggagcg ggttcttgtg ggcgttcatg aacttcacga actccatcag gccgccttcg
     8041 taacagaacg cgtgctgttt gtcgtcccgc tcgtcctgga tgttgatgtg cgtgccgggg
     8101 ttcagaaaag ccagttcgcg caggcggttg gccagcgtct caaaggaata tttgatgtcg
     8161 ccgaagatct cggggtcggg gtggaaggtc gtgcgggtgc cgcgttcgtc ggttttgccg
     8221 aggggcttca cgtctccggt gggtttgccg cgcttgtagc tctgggtgta ttttttcccc
     8281 tcgcggtaaa cttccacgtc cagccagtcg gacaacgcgt tgacgcagga cacgccgacg
     8341 ccgtgcaaac cgccggagac cttgtaggcc ttatggtcga atttgccgcc ggcgtggagc
     8401 acggtcatga cgacttccaa agcggacttg ttcttgagtt tggggtcctt gacgtccttc
     8461 atggggtcca cgggcacgcc gcggccgtcg tcttgaacgg tgatggagtt atcttcgtgg
     8521 atgatgacgt ccacgttctt ggcgtagccg gcgaggacct cgtcgatgga gttgtccacg
     8581 acctcataca ccaggtgatg gagcccgggg gccccggtgg agccaatgta catggcgggg
     8641 cggcggcgga cggcttcgag cccctctaaa acctgtattt ttgacgcgtc gtaatcgccg
     8701 gaactgcccg atgcgcgagt caccggtttc tcctcaactt tggctcccac aagaaaacct
     8761 cctcaaaaaa acgttcaccg cggatctttt ttatggaccg gccggcgcgt tccgcttttc
     8821 tcgcggaagg tcattgtccg tcggacggac ggattttgcg ccaactgtag aattatatca
     8881 gatatgacgg gccgtgtgcc aaaatgctcg ttcaattttc tgagcagggt ccgttttcgc
     8941 agcatcaagt cgtgcaggcg ggcgctggaa tccacctcca cgcagaggcg gcggccctgc
     9001 aggcccacgg ccttggcccg cgtggcctgg acgcccaaca ggcggtccca tatctcgaaa
     9061 acggcgtagt t
//
LOCUS       DAOTFD010000090         9009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5487_length_9009_cov_4.538865, whole genome shotgun sequence.
ACCESSION   DAOTFD010000090 DAOTFD010000000
VERSION     DAOTFD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5487_length_9009_cov_4.538865"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="P5079_06495"
     CDS             <1..856
                     /locus_tag="P5079_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29672.1"
                     /translation="ARAEFLRETKDENWGDGLFDYRKYTGGVETEYSYSRQIGGRFAY
                     DFYTLDFPNYRSLESSQDSTLARELAGQDVLNSNNHLFTLSGWAPLPSAVRMELTAYY
                     NRRDYGEQPVVDASGSLTGTDRSDTNLTAAVDFARPWALGDSVRLVSGLQVGYAATDS
                     NQNRYDARKTVFLRDYYDYTQMSVGPQITAGLANLPWMLTASGNYSVRNYSDRPIQDA
                     NGDYLSADTEVTNITTSLGMTYALNRNFKARAVATLGWSDSNQKYEKVFVYNYKIANY
                     LFGFSYEY"
     gene            846..7202
                     /locus_tag="P5079_06500"
     CDS             846..7202
                     /locus_tag="P5079_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY29673.1"
                     /translation="MNIKRPLRVCRNAVVRGFVALGWLAAFSVAALPLRAGQIEGQVD
                     YDGVLTPSGFSLRVVISTPSSNGGQELNRVSLSTAAFPASYSFSGLTENTTYWVWAFL
                     DIDDNLNPNDAEPMGGMGPFPFFLPEPTYVGTVAESTKTYNFNLYDRGVISVNLTNTS
                     AQSGVIILNAHPEPFVQGDENRVRRIYAGPGYYELEGLLPGVNYKVESYIDKSPFNNQ
                     WDPLEDKGESTEQVTVSTGTPPAWYGPWDVTITSANSQATGGAPNGIELRDEFGSSYQ
                     ALVAGSTSTRLRLTVMDDYWRPTISTKATDVTFNVWGPSGNYAVDLSTNAGAFHFSTG
                     TKQISIISASVSTSPYIQFVAPTDEGWYNLEAWADDFPETGNSRWAWYNFRVLSVGSG
                     INITSFRTDSGTPEVDNAIPLTPNWDGSNDAMVLEFTSPSDLQWECVISTVNFDVNAE
                     VALRPENVVARQGGWGWGGTNSVWWDGYNQNDDWRKVPNGTYYVRVQAAGGAVVSPVK
                     FVNVETGLLGGVVKSGGSPVADVRVDCWGPNSGGYAVTDANGMFKIAGLTIGSYYTLN
                     FSKAGYATTRLENKQASGGSGNNLGDITLGAGGTLQVNVAISTPPLYDVWGNVSVHDS
                     NWINTGWGNVHVRAGTTTADNGRWYQDENNKDPEYATYTVVGMAPGDYTVEFDLPQYG
                     CQTYPSLVTLGVNTTTYISFTATRKANVSGALTLPAPYAGNGGCSTCGDWISVDAVKS
                     GSDQPSAWGGGNIMPGATSTTFRVDGLDPGTYVLRAYARGYKSQTKTITVGATDLTAD
                     FDTFQTGGIITGKVTVIGNSVGLGSQTSGGWTGGCGEGKFSVNVNAWSPNTYMGSYQQ
                     ICVDESASSSEANIDMRGLDDGLYYIWTWLPGFQIEPPEMPKRVTVENGAGTANITFR
                     QLSGVLNLSVSLPAGHPNPALVSYQLQSYGGGDSTSKSGVLGAGGTAQVTGLGTGFYS
                     LNLSDAYSGLKKRAGLTVTNGRTTSLAVDMSVATKRIKGSVAVNGTISLITRSTDSLP
                     TTVNVSSATGLYKHSTFLQYDTQAMQFNEMHAPRIQVFSLPLSKHSSSPPDPYFEANA
                     STGDACIGLVASTTTDACFDFGGFVQGMYLVKVQEDLDPLPTNTNCPDCEQPGKPDLA
                     TTDKTVFINDANVTGVGLKVTNGVKLSGTISRPAGDTDTTVRTFTIKLRREDNSAVWM
                     ATAATTGVGTAAYEIPHLGEGDYNLELQEGDPFTYLPNAPAPKYTLAPKRITVGSSDL
                     VQNLTLLQAGAIVGKLRDADSNTLITSRNIEQFIPDGFYINANANPWVPGGYGWTEWD
                     MSHIKISSVTNQFAIYRLLPGNVFDVTARMENKSGGFSMSSAAQGKKAYSPVTKSGVR
                     VAAGQTVDIGVLDLKQGVSVTGTVTDKNGTPLSNILVVARPSLQEGGSSWDSQIQAFT
                     DADGKYTLMGIDRSQLYYDIIASPRFSSGDGSFFNQMSGIKYGEERIRMVDVTDDARR
                     AGVNFSLTEASGMLVGRVVTVDGGTMSTPFGDSSRDENNRKAAVMLHLAGVPLADNPL
                     GEIEEETDVMGNFQVNGLKPGSYTVRIISIGYASAKKNIVIQAGSNDIGTITLQKGAT
                     VSGKITKPNGSYPNSEEAGMVVGVDEDFEEFNFGNVETNETTKEVTGYKITGFKPGVT
                     YALLMVAEEGNDIVLGKTGLTFASVTDNKTINLVYRPVTPAVYASQSRSGSIYTLRFF
                     CTQKLRNLTADDNDLTKIVTLAAGGGQILYSSSTIGSSRDTISVVYSAPANERSFRLR
                     LSFSTIKEDPDTGSNFIFAKEFKFYAGARRMRIVQIANLMGGEAMLQGDPTGSNYISG
                     AFEVDRSSRVEVGVVSAASLDDLSAAPAAGPLAPAARAQSVKDASLHLGPEAYPSPEL
                     YSAVQMAPAVSPFSAFYDIFLPAGVNHQLKKQALLSLKYDDSVTDTAKLNVYYYDPNN
                     NVFLIEKEDRVVDAVNKTITVGVNHASTFVVLNNDAAIVTGNTYNGTEILIHNFPNPF
                     NLKPKTVNLSKSATTPSATVDGTMIRYALPAGKAGTVKFEIYNVAGELVRTITETAPT
                     GGNFYYTEWNGRNDGGKQVASGVYLGRFTINGGDERFFKMAVLK"
     gene            7292..8164
                     /locus_tag="P5079_06505"
     CDS             7292..8164
                     /locus_tag="P5079_06505"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29674.1"
                     /translation="MFASNKNTGTTGAQFLKIGAGARPVGMGEAFTALADDVNAVAWN
                     PAGLGKLTSPEFTAMHTQWFQDSDYEFVAAAYPSKWGTFGIGMASFAVDKIEKRLADT
                     DVPDGTFESKDAAYTLSYGLGLGEDWAAGVNAKYLRQSLDGRSATGLAADLGGLWRTP
                     HRPLSLGLTVRHLGSEVKFVDEGDPLPLTITLGASYKLFQDRLSVGLDLRQPKDNDLQ
                     YGVGTEYVQPCFWDLKGSLRAGYNSAGTDPTDELTGISIGAGLAWRSWGFDAAWVPYG
                     VLGQTFRYAFLVKF"
     gene            8288..8980
                     /locus_tag="P5079_06510"
     CDS             8288..8980
                     /locus_tag="P5079_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRY29675.1"
                     /translation="MAVLYDFQRLVSEARRAARWGADALEIRADCFPRSSLKPEILRP
                     ALALVREETSLPLIFTLRSRAEGGRLPRWFGEQNRLTLFRAVFPEVTVVDVELSAGDI
                     VRNVVTESHKRGRYVILSAHNFSRTPSDRELRLLSKKARRLGGDIFKVAAKVTTREEM
                     ERLMGFCRQLPFRYKVFLPMGPLGTETRLQGLFWGSALSYGYVDAPGATGPVALKSIV
                     AAHHALRRRPPA"
BASE COUNT         1770 a         2906 c         2634 g         1699 t
ORIGIN      
        1 cgcccgggcg gagttcctgc gcgaaaccaa ggacgagaac tggggcgacg gcttgttcga
       61 ttaccgcaaa tataccggcg gggtggagac cgaatacagc tattcccggc agatcggcgg
      121 ccggttcgcc tacgatttct acaccctgga tttccccaac tatcggtcgc tggagtccag
      181 ccaggattcc accctggcgc gggaactggc cggtcaggac gtgctgaaca gcaacaacca
      241 tttgttcacc ctgagcggct gggcccccct gcccagcgcc gtccgtatgg aattgaccgc
      301 ctactataat aggagggact acggggaaca gcccgtggtg gacgcctccg gcagcctcac
      361 cggaaccgac cgctccgaca ccaacctcac cgccgcggtg gatttcgccc gcccctgggc
      421 cctcggcgac agcgtccgcc tggtcagcgg cctgcaggtg ggatacgccg ccaccgattc
      481 caaccagaac cgatacgacg cccgtaaaac ggtttttctg agagattatt acgactacac
      541 ccagatgtcc gtgggtccgc agatcaccgc cgggctggcc aatctgccct ggatgttgac
      601 cgcctcgggg aattacagcg tccggaacta ttccgaccgg cctatccagg acgccaacgg
      661 cgactacctc tccgccgaca cggaagtgac gaacatcacg acgagtctcg gcatgacgta
      721 tgccctcaac aggaatttca aggcccgcgc cgtggccacc ctcggttgga gcgactccaa
      781 ccagaaatac gaaaaagttt tcgtgtacaa ttacaagatt gccaactacc tctttgggtt
      841 ctcgtatgaa tattaagcgt cctcttcgcg tctgccggaa tgccgtcgtc cgcgggtttg
      901 tcgccctggg atggctggcc gcgttttccg tggccgcttt gcctctccgc gccggacaga
      961 tcgaggggca ggtggattat gacggggttc ttaccccgag cgggttttcc ctccgcgtgg
     1021 tgatctcgac cccctccagc aacggcggac aggaacttaa ccgggtgagc ctctccaccg
     1081 ccgcgttccc ggcctcctat tcgttctccg gccttacgga gaataccacc tattgggtat
     1141 gggcgttcct cgatatcgac gacaatctga accccaacga cgctgaaccg atggggggca
     1201 tggggccctt cccgttcttc ctgccggagc cgacatacgt cggcacggtg gcggagtcca
     1261 cgaaaaccta taacttcaac ctttatgacc gcggggtcat ctccgtcaac ctcaccaaca
     1321 ccagcgcgca gtccggggtc atcattctga acgcccatcc ggagcctttc gtgcaaggcg
     1381 acgagaacag ggtccgccgg atctacgccg gtccggggta ttacgaattg gaggggctgt
     1441 tgcccggggt gaactataaa gtcgagtcgt atatcgacaa atcgcctttc aataatcaat
     1501 gggatcccct ggaggataaa ggggaatcga cggaacaggt cactgtctcc accggcacgc
     1561 cgccggcgtg gtatgggccg tgggacgtca ccatcaccag cgccaattcg caggccaccg
     1621 gcggcgcgcc caacggcatc gaactgcgtg acgaattcgg cagttcctac caggccctgg
     1681 tggccggctc cacctccacg cgcctgcgtt tgaccgtgat ggacgattat tggcggccca
     1741 ccatctccac caaggccacg gacgtgacgt tcaatgtgtg gggccccagc gggaactacg
     1801 ccgtcgatct ttccaccaac gccggagcgt tccatttttc cacggggaca aaacagatca
     1861 gcattatctc ggccagcgtc agcacttccc cgtatattca gttcgtggcg ccgaccgacg
     1921 agggctggta taacctggag gcctgggccg atgattttcc cgagacgggc aattcccgct
     1981 gggcctggta caatttccgg gtcctttccg tcggcagcgg catcaacatc acctctttcc
     2041 gcaccgattc cggcaccccc gaggtggaca acgccatccc gctgaccccc aactgggacg
     2101 gctccaacga cgccatggtg ctggagttta cgtccccgtc ggacctgcag tgggagtgtg
     2161 tgatatcgac cgtgaatttc gatgtcaatg ccgaggtggc cctccggccg gagaacgtgg
     2221 tggcgcggca gggcggctgg gggtggggcg gaaccaactc ggtgtggtgg gacggctaca
     2281 accagaacga cgattggcgc aaagtgccca acggcaccta ttacgtgcgg gtgcaggcgg
     2341 ccggcggagc cgtcgtttcg cccgtgaaat ttgtgaacgt ggaaaccggg ctcctcggcg
     2401 gtgttgtcaa gtcgggcggc tcccccgtgg cggatgtccg ggtggattgc tggggcccca
     2461 acagcggcgg ctatgccgtt accgacgcca acggcatgtt caagatcgcc gggctcacca
     2521 tcggcagtta ttacacgttg aatttttcca aggcgggtta cgccaccacg cggctggaga
     2581 acaagcaggc gtcgggcggt tcgggcaata atttgggaga catcacgctg ggcgccggcg
     2641 gcaccttgca ggtgaacgtc gccatcagca cgccgccgct gtacgacgtg tggggcaacg
     2701 tgtccgtgca cgattcgaac tggatcaaca ccggctgggg caacgtccac gtccgcgccg
     2761 gcaccaccac cgccgacaac ggccggtggt atcaggacga gaacaacaag gaccccgaat
     2821 acgccaccta cacggtcgtc ggcatggcgc cgggagacta cacggtcgaa ttcgatttgc
     2881 ctcaatacgg atgccagacg tatccctccc tcgtgaccct gggggtcaac accacgacct
     2941 acatctcttt cacggccacg cgcaaggcca acgtgagcgg ggcgttgact cttccggccc
     3001 cctacgccgg caacggcggg tgttccacct gcggcgactg gatctcggtg gatgccgtca
     3061 agtccggttc cgaccagccc tccgcctggg gcggcggcaa catcatgccg ggcgccacct
     3121 ccaccacgtt ccgggtggac ggcctggatc cgggtaccta tgtgttgcgc gcctatgccc
     3181 gcgggtataa atcgcagacc aaaaccatta cggtcggcgc caccgatctt accgccgatt
     3241 tcgacacctt ccagaccggg gggatcatta ccggaaaagt gacggtcatc gggaacagcg
     3301 tcggactcgg gagccagacc tccggcggct ggaccggtgg ctgcggcgag ggtaaatttt
     3361 ccgtgaacgt caacgcctgg tcgcccaaca cctatatggg gtcctatcag cagatctgcg
     3421 tggacgaaag cgcctcctcc tcggaggcga acatcgacat gcgcggattg gacgacggcc
     3481 tctactacat ctggacctgg ctgcccgggt tccagatcga gccgcccgaa atgcccaaac
     3541 gggtcacggt cgagaacggc gcggggacgg ccaacatcac tttccggcaa ttgtcgggcg
     3601 tcctgaacct gagcgtctcc ttgccggccg gccatcccaa ccccgccctg gtgtcctacc
     3661 agctgcagag ttacggcggc ggggactcaa cgagcaaatc gggcgtcttg ggcgcgggcg
     3721 ggacggcgca ggtcaccggc ctgggcacgg gtttttattc gctgaatctg tccgacgcct
     3781 acagcggctt gaagaagagg gccgggttga ccgtcaccaa cggccgcacc acctcgctgg
     3841 ccgtggacat gtccgtcgcc accaaacgca tcaaggggtc ggtggccgtg aacggcacca
     3901 tttctttgat aacgcgaagc acggactctt tgcccaccac ggtcaacgtc agttccgcca
     3961 cgggcctcta taaacattcc actttccttc aatacgatac gcaggccatg cagttcaacg
     4021 aaatgcacgc cccgcgcatc caggtgtttt ctctgccgct ttccaaacac agcagcagtc
     4081 ctccggatcc ctatttcgag gccaacgcct ccaccggcga tgcctgcatc gggctggtgg
     4141 cctccaccac caccgacgct tgtttcgatt tcggcgggtt tgtgcagggg atgtatctgg
     4201 tgaaggtcca ggaggacctc gaccctctgc cgaccaacac caactgtccg gattgcgaac
     4261 agccggggaa acccgatctg gccaccaccg acaaaacggt tttcatcaac gacgccaacg
     4321 tcaccggcgt gggcctgaag gtgaccaacg gggtgaaact ttccggaacc atcagccggc
     4381 cggcggggga tacggacacg acggtccgta ccttcaccat caaacttcgc cgggaagaca
     4441 actccgccgt gtggatggcc accgcggcca ccaccggcgt cggcaccgcg gcttatgaga
     4501 tcccgcattt gggcgaaggg gactacaacc tggaacttca ggaaggcgat ccgtttacgt
     4561 atctccccaa cgcgcccgcc cccaaatata cgctggcccc gaaacgcatc accgtcggct
     4621 cttccgacct ggtgcagaac ctcacgctcc tgcaggccgg ggccatcgtc ggaaaattgc
     4681 gggacgccga ctccaacacc ctcatcacct cgcgcaacat cgaacagttc attcccgacg
     4741 ggttctacat caacgccaac gccaacccct gggtccccgg cgggtacggc tggaccgagt
     4801 gggacatgtc ccacatcaag atctcctccg tcaccaacca gttcgccatc tatcggctcc
     4861 tgcccgggaa cgtgttcgac gtgaccgccc gcatggagaa caagagcggc ggcttcagca
     4921 tgagttcggc tgcccagggc aaaaaggcct attctcccgt caccaagtcc ggggtgcgtg
     4981 tcgccgccgg tcaaacggtg gacattggcg ttctcgatct gaaacagggc gtcagcgtca
     5041 ccggcaccgt cacggacaag aacggaacgc ctctgtccaa catcctggtg gtggcgaggc
     5101 cgtcgcttca agagggcggt agcagctggg attcccagat acaggctttt accgacgccg
     5161 acggcaaata cacgttgatg gggatcgacc gcagccagct ttattacgac atcatcgcct
     5221 ccccgcggtt cagttccggc gacgggagtt tctttaacca gatgtccggt atcaaatacg
     5281 gggaggagcg catccgcatg gtggatgtga ccgacgacgc ccgccgtgcc ggcgtcaact
     5341 tcagtcttac cgaggccagc ggcatgctgg tggggcgcgt cgtcacggtg gacggcggca
     5401 ccatgtccac ccccttcggc gacagcagcc gcgacgagaa caaccgcaag gcggcggtca
     5461 tgctccacct ggcgggcgtc cccctggccg acaacccctt gggcgagatc gaggaagaga
     5521 ccgacgtcat ggggaacttc caggtcaacg gtttgaagcc gggttcctat accgtgcgca
     5581 tcatctccat cggatacgcc tcggccaaga aaaacatcgt gattcaggcc gggtccaacg
     5641 acatcggtac catcacgctc cagaaaggcg ccacggtcag tggaaagatc accaaaccca
     5701 acggcagcta tccgaacagc gaagaggccg gcatggtggt cggtgtggac gaggatttcg
     5761 aggagttcaa cttcggcaac gtcgagacca atgagaccac caaggaggtc accgggtata
     5821 agatcaccgg tttcaagccc ggcgtgacct acgcgctcct tatggtggcc gaggagggga
     5881 acgacatcgt gttgggcaag acggggctca ccttcgcttc cgtcaccgac aataagacca
     5941 tcaacctggt ttaccgaccc gtgacgcccg cggtgtatgc cagccagtcg cggtcgggca
     6001 gcatctacac cctccgcttc ttctgcacgc agaagttgcg caacctcacc gccgacgaca
     6061 acgatctgac caagatcgtc acgttggcgg ccggcggcgg gcagatcctc tattccagca
     6121 gcaccatcgg cagttcgcgc gataccatct ccgtcgtcta ttcggcgccc gccaatgagc
     6181 ggtcgttccg cctccgtctc tcgttctcca ccatcaagga agatccggac acgggttcca
     6241 atttcatctt tgcgaaagag ttcaagttct acgccggcgc gcggcgcatg cgcatcgtgc
     6301 agatcgccaa cctcatgggc ggcgaggcca tgctgcaggg cgaccccacc ggaagcaact
     6361 acatctccgg agcttttgag gtggaccgct cttcccgcgt ggaggtgggc gtggtgagcg
     6421 ccgcgtccct cgacgatttg tccgccgcgc cggccgccgg gccgctcgcg cccgccgccc
     6481 gcgcccagag cgtgaaagac gcctctctcc acttgggtcc cgaggcctat ccctccccgg
     6541 aactctattc cgcggtgcag atggctcccg ccgtgagtcc cttcagcgcg ttctatgaca
     6601 ttttcctgcc ggccggggtg aaccatcaat tgaaaaaaca agccctgctt tcgttgaaat
     6661 acgatgattc cgtcacggac accgccaagt tgaacgttta ctattatgat cccaacaaca
     6721 acgtgttcct catcgaaaaa gaggaccgcg tcgtggacgc cgtcaacaag accatcaccg
     6781 tcggcgtcaa ccacgcctcc accttcgttg tgctcaacaa cgacgcggcc atcgttacgg
     6841 gcaacaccta caacggcacg gagatactca tccataactt ccccaacccg ttcaatctga
     6901 aaccgaagac ggtgaacctc agcaagtccg ccaccacccc gtcggccacc gtcgacggga
     6961 ccatgatccg ttacgccctc ccggccggga aggcgggcac cgtgaaattt gagatctaca
     7021 acgtggcggg ggaactggtc cgcaccatca ccgaaaccgc tccgaccgga gggaatttct
     7081 attacaccga gtggaacggc cggaacgacg gcggcaaaca ggtggcctcc ggcgtctatc
     7141 tcggccggtt cacgatcaac ggcggcgatg agcgcttctt taagatggcg gttctgaaat
     7201 gaaaaaaagt ttaaggttta aagtggaagg tttaaagcgg gccgcaatgg ctttggcggt
     7261 tacttttaac tttacacttt taacctttaa cgtgtttgct tcaaacaaaa acaccggcac
     7321 caccggggcg cagttcctca agatcggcgc cggcgcccgg cccgtgggca tgggggaggc
     7381 cttcaccgct ctcgccgacg acgtcaacgc cgtggcgtgg aaccccgccg gattggggaa
     7441 gttgacctcc cccgagttta ccgccatgca cacccaatgg ttccaggact cggattatga
     7501 gttcgtggcc gcggcgtatc cctcaaaatg ggggactttc ggcatcggca tggccagctt
     7561 tgcggtggac aagatcgaaa aacggctcgc cgacacggac gttcccgacg ggacgttcga
     7621 atccaaggac gccgcttaca cgctttccta tgggcttggc ttgggtgagg attgggccgc
     7681 cggcgtcaac gccaaatatt tgcgccaaag cctggacggc cggtccgcca ccgggctcgc
     7741 ggcggacctc ggcggcctct ggaggacccc gcatcggcct ctgtccctcg gacttaccgt
     7801 gaggcatttg gggtcggagg tgaagttcgt ggacgaggga gaccctctgc cgttgacgat
     7861 caccctgggc gcctcttata aactgtttca ggaccgcctt tcggtcgggt tggatctccg
     7921 ccaacccaag gacaacgatt tgcagtacgg cgttgggacg gaatatgtcc agccctgttt
     7981 ctgggacctg aagggcagcc tgcgcgcggg atacaactcc gccggtaccg atcccaccga
     8041 cgagctgacc gggatctcca tcggcgccgg attggcctgg cgcagctggg gtttcgacgc
     8101 cgcgtgggtc ccctacggcg tcctcggcca gacgttccgt tacgccttcc tcgttaagtt
     8161 ttaatctcct atcgaacatc tttccgttgg cgaaacccgc tggcttttcc cgtccgttct
     8221 ccctgtttga ccgatgagcg cccgtcgtcg accgccgttg cccggtctca atcggcgtcc
     8281 gttggccgtg gccgttcttt acgatttcca acgcctcgtt tcggaggcgc gccgggccgc
     8341 ccggtggggg gccgacgccc ttgagatccg cgccgattgc tttccgagga gttcgctcaa
     8401 gccggaaatc ctgcgcccgg cgctcgccct cgttcgagag gaaacgtcgc tcccgttgat
     8461 cttcaccctc cgttcccgcg ccgaaggcgg tcgactgccg cgttggttcg gagaacagaa
     8521 ccggttgacc ctgttccgcg cggtgtttcc ggaagtgact gtggtggatg ttgaactcag
     8581 cgccggcgac atcgtccgaa acgtcgtcac cgagtcccat aaacgcggaa ggtacgtgat
     8641 cctctccgcc cataattttt cgcggacgcc ctcggatagg gaactgcgtc ttctttcgaa
     8701 aaaggcccgg cgtttgggag gggatatctt caaggtggcg gcaaaagtca ccacacggga
     8761 agaaatggaa cgtttgatgg gtttttgccg tcagttgccg tttcggtaca aggtttttct
     8821 ccccatgggc cctctcggca cggagacgag gttgcagggg ctcttttggg gctccgctct
     8881 gagctacggc tatgtggacg ctcccggggc gacgggaccc gtcgccctga aaagcattgt
     8941 cgcggcccat cacgctctgc gtcgtcgtcc ccccgcataa ccctcgatac aaaaagtggt
     9001 atactaacc
//
LOCUS       DAOTFD010000091         8975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5508_length_8975_cov_4.814013, whole genome shotgun sequence.
ACCESSION   DAOTFD010000091 DAOTFD010000000
VERSION     DAOTFD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_8975_cov_4.814013"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5079_06515"
     CDS             complement(<1..194)
                     /locus_tag="P5079_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRY29676.1"
                     /translation="MSTHEVHLEVFEGPLDLLLHLIQKDDLDIYDIPVSQITSEYLAY
                     MDMMKDLKLDVAGEFLVMAAT"
     gene            complement(208..1194)
                     /gene="trpS"
                     /locus_tag="P5079_06520"
     CDS             complement(208..1194)
                     /gene="trpS"
                     /locus_tag="P5079_06520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY29677.1"
                     /translation="MTKKIILSGIRPSGKLHLGNYWGALSRWLELQNNSAYQCYFMVA
                     DLHALTTGYDRTADLRSNTEEMVLDWLAAGLDPAKSVLFRQSDVAEHAELSLLLSMIT
                     PLGWLERIPTYKEQLRELAEREITTHGFLGYPVLQAADILVYKADAVPVGEDQLSHLE
                     LCQEVARRFNRLYGPVFSEPKALLGETPKVPGLDARKMSKSYGNSVELSDSPEILQKK
                     VSQMYTDPKKIRANDHGHPEGCVVFALHKIYNPEYVAREAECREGRIGCVACKKHLLS
                     LMDPALRPFRERRAALEGDRGAVWKVLEEGNAKARQAARRTLDEVKRAMGLA"
     gene            complement(1265..2482)
                     /locus_tag="P5079_06525"
     CDS             complement(1265..2482)
                     /locus_tag="P5079_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29678.1"
                     /translation="MADKKNILNRLPALWAGRFRALGRLAEERGLTLCLVGGCVRDLM
                     LAVPPLDWDAVVQGPAGGLVQESARLFKAEVVQHPSFLTYTLLFADGTALDVATARSE
                     TYPHPAALPVVEPSSLAEDFARRDFTVNAMAVHLSPGRWGRLEDPFDGRKDLRRGLVR
                     VLHAKSFMDDPTRLFRAARYAGRNGWTLDGATLRLFLEAIKQDGPALLSRARRRTELE
                     RLLGEHEPRPALKMLWKSGLWGYWNKDWRWSPFYAKWLSAASSSGLAPAERVLFRLMV
                     LCRFRGAEEARRDLMALEFPRNTVESVTHAVHLREAIAGEKFASLVSSPKTAAAVKAF
                     LRASLRRPAALRRWERSVPLLGGEDLRELGFRPGPVYQKIFESLRRARWEGRVRNRAD
                     EVRHVIDNFSRNQ"
     gene            complement(2472..4067)
                     /locus_tag="P5079_06530"
     CDS             complement(2472..4067)
                     /locus_tag="P5079_06530"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRY29679.1"
                     /translation="MPPRRTRPKDGLAPPAPIDPAGDAGFSEAYLVLRDRLLKTKKTS
                     SSAGDGLLADAVSLNEKTLRTVWYEGRFERRGLRTVDGRRVGLVTPGEWNLDEGPDFR
                     GAEIRLEDRLVRGDVEIHLLDKGWKSHGHHEDPHYDNVVLHVVLQTSRVPAPAVTRRG
                     MAVPTVVLGDRLPHPWLEVVQKFDAAGYPFNSHCGLGDCGRKVHLAKYDLLRHLLSCA
                     GDGRVLLKAGSLGPVDGTSSLADVFYRRLLESAGYHRNKRGMLALAGRLRWKTLKDAV
                     SSAGPSRRALLEALLFSFSGLLPETAPASDPETADLLRGMLSLRERVEPALPRELVSP
                     DRESNDSGAWVFRGVRPHNFPCRRVSGTAALLDRFFARLRETPQEDVALLDFGRAGPD
                     AFFVAGEGYFGRRAVWGGKKFRHPAALIGEDRAQAMWVNVYLPLALRVARRRGDAKLE
                     KELHARYHRLRLSEPNGAARLMAHRLFGAERVRRFRLNGEFEQQGLLQIFQDFCDTKP
                     WACRHCVFPQTLDMSLAEMEHGR"
     gene            complement(4069..4452)
                     /locus_tag="P5079_06535"
     CDS             complement(4069..4452)
                     /locus_tag="P5079_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29680.1"
                     /translation="MLDEVRSDSVVQAPPGGLRKSWFHSAEGDLYVWLNGQNDVRSFQ
                     LSFFAGGEERTECWVEWDGRQRRVGTVDSREVDSRPHDIRSPMVELSEAPRKRIIEQA
                     AEFLDREGGNLPEGLRTFVRKKVAA"
     gene            complement(4458..5126)
                     /locus_tag="P5079_06540"
     CDS             complement(4458..5126)
                     /locus_tag="P5079_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRY29681.1"
                     /translation="MSTCLEQDINCPCGEEFDARLWSSVNTQTDPALRDEILAGQLNV
                     VKCPRCRRFIYAERFVLYHDPSVELMVFVYPAAYASEEERWRKKTEEDYKSVLGAVNE
                     KERLPYGALSVFGLDRLVELLRREQAAADEEAVFESLAPSLSLQIGCLGSSVARENAL
                     PSRLPLSGDATDSPEDRLREGLRVLLAANDRLASYAAAAERLARRAFDPAGLLSGHSQ
                     KKGG"
     gene            complement(5169..6725)
                     /locus_tag="P5079_06545"
     CDS             complement(5169..6725)
                     /locus_tag="P5079_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29682.1"
                     /translation="MAPLANPLSALERSDTPWNLAESFLEAGETAAVEKFHARLEGPK
                     KLDTARRVASLLELYRGNYGPARDRLREISGQDEWCLSRGKYLDGLLAASDDFVETGS
                     EHFRIRTSPNDAFLAEYVQRALENSHSKMAEVFHLEPSSGVVVEIYPTETRFSRASTL
                     SPETLERSGAIGICKFRRLMVLSPQATPMGYRWLDALSHEYVHFLINEISGTLCPLWL
                     HEGVARYYETAWRRAGPFEHTPAAETQLARAAQKQPDDPSALVRFQRMEPSMVYLDNQ
                     EQVSLAFTEVSDAVAYLLEEFSPEKLFELLAAFRKYPREEAFERMLGMSEEDFEEAWR
                     DSLKDRSWVTSRGAMDQKISLRPMDETEFLGPDVRGHIRLGDRLRQQDQMAAALVEYK
                     KALEREPDNGVALLKTARVFLALGNESEAEEALRHAVKKNPAYVTPYAVLGGVLSEQG
                     RYEEAQPVLEEGLEINPFHPELHRALGLIAVDIGHFSAARKSLELALRLNPNDSELRE
                     VLRRMPKESR"
     gene            complement(6835..>8975)
                     /locus_tag="P5079_06550"
     CDS             complement(6835..>8975)
                     /locus_tag="P5079_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29683.1"
                     /translation="FLNYKINDGPVMKTEWEWGSAGRAEKAANGDFDHVWSLRELGLR
                     AGDTVRYHLSAKDNNAVSGPSESQSAAFLLRVASYEEEHERMERELAEAQKELLGLLA
                     EETLLRENLNAAPVDWEGLDRSQAALRHRTAAQAKRFDALVQKIRTDPLMDEAAAIEY
                     EGVARNLTELERDAMARAEEAVRSRQKESAAPAMDEIVSELERLSLLAEDAQQARRMR
                     DVLARQDDMTRAAENLLDELSSRPAGAFSDEEMKRWAETMAEIARLTREISEQIQKMP
                     KELPEDFVNQPGVKNLRFDEVQSAAEELRRALSAGSSAGALAAAKQLLDRLKEIQKNL
                     EDSARQSPGSSSWYGDELMGDIGKRQKELQGLIERQEKIKSGTDALSRSALAGLLERQ
                     KTLMGEVSVDLDRWDPRFREFQERFSSSVEPVFRLQNMARFQRAEALLRSAREELGRR
                     DLQNTPAGLEELHSKLQEVEGALREEKLRLTLEDGGKPSANGTARTLRPAELPQETRR
                     VYELLEGLEKTAGELGGDLDRQIRKLRTPPRESEFLSSDERKSAQELSSAQRMLKDDT
                     SVLRQDLRELARKTATLSPRLDRRLFLAAQAMTEAADRLEEESLRKAADAEETALRYL
                     RETEQEMQSAAQNAAGMESGAGRSPAGSIRVLSGSGAAGARLEPVRIPRAEDYRPPRE
                     FREELLKSMREKYPEIYEDIVQDYYEHWAK"
BASE COUNT         1620 a         2984 c         2749 g         1622 t
ORIGIN      
        1 gtggccgcca tcaccaggaa ctcgccggcc acgtcgagct tgaggtcctt catcatgtcc
       61 atgtaggcca ggtattccga ggtgatctgg gagacgggga tgtcgtaaat gtcgaggtcg
      121 tctttttgga tgagatgcag caggagatcg agcggcccct cgaagacttc cagatggact
      181 tcgtgcgtgc tcaccggccc ctctccatca ggccagcccc atggcccgct tgacctcgtc
      241 cagggtgcgg cgggcggcct ggcgcgcctt cgcgttcccc tcttccagga ccttccacac
      301 ggcgccccgg tcgccctcca gcgcggcccg tctttcacgg aaaggacgca gcgcgggatc
      361 catcagggag agaagatgtt ttttacaagc cacacagccg atgcggcctt cccggcattc
      421 cgcctcccgc gcgacgtatt ccgggttata gattttatgc agggcgaaaa cgacgcagcc
      481 ttccggatgg ccgtggtcgt tggcccgtat cttcttgggg tcggtgtaca tctgcgacac
      541 ttttttctgc aggatttccg gggagtccga cagttccacg gagttcccgt aggatttcga
      601 catctttcgg gcgtccaggc ccggcacctt cggagtctcc cccaagagag ccttgggttc
      661 ggaaaaaacc ggaccgtaga gccggttgaa ccgccgggcg acttcctggc acagttccaa
      721 atgggacaat tggtcttccc ccaccggcac ggcgtcggct ttgtagacga ggatatccgc
      781 ggcctgaagg accggatagc cgagaaaacc gtgcgtggtg atctcgcgtt cggcgagttc
      841 ccgcaactgt tccttatacg tcgggatgcg ttccagccat cccaaggggg tgatcatgga
      901 cagaagcagg gaaagctcgg cgtgttccgc cacgtccgac tgacggaaga gcacgctctt
      961 ggcggggtcg agtccggcgg ccagccaatc gagcaccatc tcctcggtgt tcgatctcag
     1021 atcggcggtg cggtcgtagc cggtggtcag ggcgtgcagg tcggcgacca tgaaatagca
     1081 ctgataggcg gaattgttct gcaattccag ccagcgggaa agcgcccccc aatagttgcc
     1141 gaggtgcaat ttacccgaag gccgaatccc cgacaagatg atcttttttg tcatgtcccc
     1201 tttttcgctt tcgcccgcgc gccgaaacgg cgcgacgccg gcgtcgattt atgacggaaa
     1261 aatcctattg attccgagag aaattgtcaa tgacgtgccg cacctcgtcc gcccggtttc
     1321 tgacgcggcc ttcccagcgc gcccgacgga gagattcgaa tattttctga tagaccgggc
     1381 ccggccggaa accgagctcc cgcaggtctt ccccgcccaa cagggggacg gaacgttccc
     1441 agcgccgcag ggcggcgggc cggcggagcg acgcccggag gaacgccttg acggcggccg
     1501 ccgttttcgg cgacgatacc agggaggcga atttctcccc ggcgatcgct tcgcgcaaat
     1561 gcacggcatg ggtcaccgac tccacggtgt tcctggggaa ttcaagggcc atcagatcgc
     1621 ggcgggcctc ctcggcgccg cggaagcggc agaggaccat caagcggaac aggacccgct
     1681 cggcgggggc caggccgctc gacgacgccg ccgacagcca tttcgcgtaa aagggagacc
     1741 agcgccagtc cttgttccaa tatccccaga ggccggattt ccaaagcatt ttcagggcgg
     1801 ggcggggctc gtgttcgccc aggaggcgct ccagttccgt ccgtcgccgg gcgcggctca
     1861 gaagggccgg gccgtcctgt tttatcgcct ccaagaacaa ccggagggtg gcgccgtcca
     1921 gcgtccaacc gttgcgaccg gcataacggg ccgcgcgaaa gagccgggtg gggtcgtcca
     1981 tgaaactttt cgcgtgcaag acccgcacca ggccccggcg caggtctttc cgcccgtcga
     2041 aggggtcctc caagcggccc caccggccgg gggaaaggtg gacggccatg gcgttcaccg
     2101 tgaaatcgcg ccgggcgaaa tcctccgcca ggctggacgg ttccaccacc ggaagggccg
     2161 ccggatgggg ataggtctcg gaccgggcgg tggccacgtc cagcgcggtg ccgtcggcaa
     2221 agaggagggt gtaggtcagg aaactcgggt gctggacgac ctcggcctta aaaaggcggg
     2281 ccgactcctg caccaacccg cccgccggcc cctgaaccac cgcgtcccag tccaagggag
     2341 gcacggcgag catcagatcc cgcacgcatc cccccaccag gcaaagcgtg aggccgcgtt
     2401 cttccgccaa ccggcccaga gcgcggaagc ggccggccca gagcgcggga aggcggttca
     2461 agatgttctt cttatcggcc atgttccatc tccgcgaggg acatgtccag cgtctgcggg
     2521 aacacgcagt gccggcaggc ccacggtttg gtgtcgcaaa aatcctgaaa aatctgaagg
     2581 agtccctgct gttcgaactc cccgttcagc cggaaccgcc ggacgcgttc cgcgccgaag
     2641 agccgatgcg ccatcagacg ggcggcgccg ttgggttcgc tcagacgcag gcggtgatat
     2701 cgggcgtgga gttccttctc cagtttagca tctccgcggc gccgtgccac gcgcaaagcc
     2761 agcggcagat agacgttgac ccacatggcc tgcgcccggt cttcgccgat gagggcggcg
     2821 gggtgacgga actttttccc tccccacacg gcgcggcggc cgaaatagcc ctcgccggcg
     2881 acgaaaaaag cgtccggccc cgcgcggcca aaatccaaga gagcgacgtc ctcctgcggc
     2941 gtttcccgaa ggcgcgcgaa gaaccggtcc aacagcgccg ccgtcccgga gacgcgccgg
     3001 caggggaagt tgtgcggccg gacgccgcgg aaaacccacg ccccggaatc gttcgactcc
     3061 ctgtccggcg aaacgagctc ccgaggcaac gccggttcga cgcgttcgcg gagcgagagc
     3121 attccacgga gcaggtccgc cgtctcaggg tccgacgcgg gcgcggtctc cggcagcagg
     3181 ccggaaaaag aaaacaggag ggcctccaaa agcgctcggc gggaaggccc ggcggaggac
     3241 accgcgtctt tgagggtctt ccaccgcaac cgtccggcca gggcgagcat gccgcgcttg
     3301 ttcctgtgat accccgcgct ttccaagagc cgccgataaa acacatcggc gagagaactc
     3361 gtcccatcga caggaccgag agaacccgcc tttaaaagga cgcggccgtc gccggcgcag
     3421 gaaaggagat gccgcaacag gtcgtatttc gccagatgga ctttccggcc gcaatcgccc
     3481 aggccgcaat ggctgttgaa cggatacccg gccgcatcga atttctgcac gacctccagc
     3541 caggggtgcg ggagccggtc gcccagaacg acggtcggca cggccatccc ccggcgggtg
     3601 accgccggcg ccgggacgcg cgacgtttga agcaccacat gcaggaccac gttgtcgtaa
     3661 tgcgggtcct cgtggtggcc gtgcgatttc caacccttgt cgagcagatg gatttcgacg
     3721 tccccgcgga ccaggcggtc ttccaggcgg atctccgctc cccggaaatc cggcccctcg
     3781 tccaagttcc attcgcccgg ggtcaccagt ccgacgcgcc ggccgtcgac ggtgcgcagc
     3841 ccgcgccgct caaagcgacc ctcgtaccaa accgtgcgga gcgtcttctc gttcaaagag
     3901 accgcgtccg cgagaagtcc gtccccggcc gaagaggatg tctttttcgt cttaagcagc
     3961 cggtcccgca gaacgagata ggcctccgaa aaaccggcgt cgccggccgg gtcgatgggc
     4021 gcgggcgggg cgagtccgtc tttcggtcgc gttcggcgag gcggcatctt acgccgccac
     4081 tttttttcgg acgaacgtcc ggagcccctc cggcagattc ccgccttcac gatccaaaaa
     4141 ttcggccgcc tgctcgatga tcctctttcg cggcgcctcg gacaactcca ccatcgggga
     4201 acggatgtcg tggggccggg aatccacctc ccgggaatcg acggtgccga cgcgacgttg
     4261 gcggccgtcc cattcgaccc aacattccgt ccgctcctcc ccccccgcga aaaaactcag
     4321 ctggaacgac cggacatcgt tctgcccatt gagccacaca tagaggtctc cttcagccga
     4381 atggaaccaa ctctttctga gcccgccggg cggcgcttgg acaacggagt cggaacggac
     4441 ttcatcgagc atcgaggtta tccgcctttc ttttgggaat ggccggacaa caggccggcc
     4501 gggtcgaaag cgcgccgggc caggcgttcc gccgcggccg cgtaggacgc cagccggtcg
     4561 ttggcggcca gaagcacgcg cagcccttcc cggaggcgat cttcgggaga gtccgtggcg
     4621 tcgcccgata acggcagacg cgagggaaga gcgttttccc gcgccaccga ggaaccgaga
     4681 cagccgatct ggagagaaag cgacggggcg agagattcga acaccgcctc ctcgtcggcc
     4741 gcggcctgtt cccggcgaag cagttccacc agccggtcca atccgaaaac cgagagcgcg
     4801 ccgtagggga gccgttcttt ctcgttgacg gcgcccaaca cggatttata gtcctcctcg
     4861 gtttttttcc gccatctctc ttcctcggag gcataggcgg cgggatagac gaaaaccatc
     4921 aactccacgg aaggatcatg ataaaggacg aaccgctccg cgtagataaa ccggcggcag
     4981 cgcgggcatt tgaccacatt cagttggccg gccaggatct cgtcacgcag ggccgggtcc
     5041 gtctgggtgt tgacggaact ccagagacgg gcgtcgaatt cctcgccgca ggggcagttt
     5101 atgtcttgtt cgaggcaggt ggacatgggg cgctcatcct tggaggattt tccggaaaaa
     5161 ccggcccttc aacgggactc cttcggcatt ctccgcaaaa cctcgcggag ctcactgtcg
     5221 ttgggattca ggcgcaacgc caattccagg gacttccggg ccgcggagaa atggccgata
     5281 tcgacggcga tcagcccgag ggcgcggtgg agttccgggt ggaagggatt tatttccagc
     5341 ccctcctcca ggaccggctg ggcctcctcg taacggccct gttcggagag aacaccgccc
     5401 aacacggcgt acggggttac gtatgcggga tttttcttga cggcgtgccg cagcgcctcc
     5461 tccgcctcgg attcgttccc caaggccagg aaaacccggg ccgttttcaa caacgccacc
     5521 ccgttgtccg gctcgcgttc aagggctttt ttatattcca ccagagccgc cgccatttga
     5581 tcctgctggc ggagccggtc ccccagccgg atgtggccgc gcacgtccgg ccccaggaac
     5641 tccgtttcat ccatgggccg taacgaaatt ttctggtcca tggcgccgcg ggaggtgacc
     5701 caagaccggt ccttcaaaga atcccgccac gcctcttcga aatcctcctc gctcatcccc
     5761 agcatccgct cgaacgcctc ctccctcgga tacttccgaa aagcggcgag cagttcgaaa
     5821 agtttttccg gggagaactc ctccagcaga taggccacgg cgtcggacac ctcggtgaac
     5881 gccagcgaca cctgttcctg attgtccaga tagaccatgg acggctccat gcgttgaaac
     5941 cggacgagcg cggacggatc gtcgggctgt ttttgggccg cccgggccag ctgcgtctcc
     6001 gccgccgggg tgtgttcgaa cgggccggcg cgccgccacg ccgtctcgta ataacgcgcg
     6061 acgccctcgt gcagccacag cggacagagc gtgcccgata tttcgttgat gagaaaatgc
     6121 acgtattcat gggacaacgc atccagccag cgatacccca tcggcgtcgc ctgcggcgaa
     6181 aggaccatca ggcgccggaa cttgcatatg ccgatggcgc cggaacgttc cagggtctcc
     6241 ggcgacaggg tggacgcccg ggaaaaccgc gtctccgtcg gatagatctc gacgaccacc
     6301 cccgacgacg gttccagatg gaacacctcc gccattttgg aatgagagtt ttccagggcg
     6361 cgctggacgt attccgccaa aaaggcgtcg ttcggcgacg tccgtatgcg gaaatgttcc
     6421 gaacccgttt ccacgaaatc gtccgaggcg gcaagcaggc cgtcgaggta tttcccccgg
     6481 gagaggcacc actcgtcctg tccggaaatt tcccgcagac ggtcccgggc cggaccgtag
     6541 ttcccccggt agagctccag gagggaggcg acgcggcggg cagtgtcgag ttttttaggg
     6601 ccctccaggc gtgcatggaa cttctccacc gcggccgttt cccccgcctc caaaaaggat
     6661 tccgccaggt tccagggcgt gtcgcttcgt tccaacgcgc tcaacgggtt ggccaacggg
     6721 gccactccga acgacgcacc gtttttgggg gaggacagga gggtcgccgg caagaaacag
     6781 gcggcgaaaa aaagtcccgc gcctcggcgc cgggagttcc cggtgacaaa gcgatcattt
     6841 tgcccaatgt tcataatagt cctgaacgat gtcttcatag atctccgggt atttttcccg
     6901 catagatttt aacaattctt cgcgaaattc gcggggcgga cgataatcct ccgcccgtgg
     6961 aatacggacc ggctccagac gcgccccggc agcgccgctt cccgacagca cccggatgga
     7021 cccggccggg gagcggccgg cccccgactc catcccggcg gcgttctgcg cggccgactg
     7081 catctcctgt tccgtctccc ggagatagcg aagcgccgtt tcctccgcgt ccgcggcctt
     7141 tcgcaaagac tcctcctcca gccggtccgc cgcctcggtc atggcctggg ccgccaggaa
     7201 aaggcggcgg tccaaccgag ggctcagcgt ggcggtcttc cgggccaact cccgcagatc
     7261 ttgccgcaga accgacgtat cgtccttcaa catcctttgc gccgacgaaa gttcctgggc
     7321 ggatttcctc tcatcggacg acaagaattc cgactcccgc ggcggggtcc gaagtttccg
     7381 gatctggcgg tccaggtcgc cgcccagttc ccccgccgtc ttctccagtc cttccagcaa
     7441 ttcatagaca cgccgcgttt cctgcggcaa ttcggcggga cggagagtcc gggccgtccc
     7501 gttggcggac ggttttcctc cgtcctccag cgtcaagcgg agcttttcct cgcgaagggc
     7561 cccttccact tcttgaagtt tggaatggag ttcctccagc ccggcgggcg tgttctggag
     7621 atccctccgg cccaactcct cccgggccga tcgcaagagg gcctcggcgc gctgaaaccg
     7681 ggccatgttc tgcaaccgga agacgggctc caccgacgag gagaagcgtt cctggaactc
     7741 ccgaaagcgc gggtcccaac ggtcgagatc gaccgacacc tcccccatga gcgtcttttg
     7801 tctttccagc aatcccgcca gcgccgaccg cgagagggcg tcggtgccgg actttatttt
     7861 ttcttggcgt tcgatcaatc cctgaagttc cttctgtctt ttgccgatgt cccccatcag
     7921 ttcgtcgcca taccaggaag aactcccggg agattgacgc gccgagtcct cgaggttctt
     7981 ctggatctct ttcagccggt cgaggagttg ctttgcggcc gccagggccc cggcggagga
     8041 gcccgccgag agcgcccggc gcagttcctc cgcggccgac tggacttcgt cgaaacggag
     8101 gttcttcact cccggctggt tgacgaaatc ctccggcagt tctttcggca tcttttggat
     8161 ttgttcggag atctcccggg tcaaccgggc gatctccgcc atcgtctcgg cccagcgttt
     8221 catttcctcg tcggaaaacg cgccggccgg ccgcgaggag agctcgtcga gcaggttttc
     8281 cgcggcgcgc gtcatgtcgt cctgccgcgc cagcacgtcc ctcatccgcc gggcctgttg
     8341 cgcgtcctcc gccaaaaggg aaagccgttc gagctcggac acgatctcgt ccatcgccgg
     8401 agcggccgat tctttttgcc gggaacggac ggcctcttcc gcccgggcca tggcgtcccg
     8461 ctccaactcc gtcaaattcc gggcaacccc ctcgtattcg atggcggccg cctcgtccat
     8521 cagcggatcg gtccttatct tttggaccag cgcatcgaac cgtttcgcct gagcggccgt
     8581 ccgatgacgc aacgccgcct ggctcctgtc cagtccctcc caatccaccg gggcggcgtt
     8641 caggttttcg cgcagaagcg tttcttccgc caaaaggccc aggagttcct tctgggcctc
     8701 cgccaactca cgttccatcc gttcgtgttc ctcctcatag gaagccacgc ggagaaggaa
     8761 cgcggcgctt tgggactcgg acgggccgga aacggcgttg ttgtccttcg cggaaaggtg
     8821 ataacgcacc gtgtctcccg cgcgcaggcc gagttccctc aacgaccaaa catggtcaaa
     8881 gtccccgttc gcggcttttt ccgcccgccc cgcggaaccc cactcccatt cggttttcat
     8941 gaccgggccg tcgtttatct tgtaattcaa aaaga
//
LOCUS       DAOTFD010000092         8920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5543_length_8920_cov_4.150254, whole genome shotgun sequence.
ACCESSION   DAOTFD010000092 DAOTFD010000000
VERSION     DAOTFD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5543_length_8920_cov_4.150254"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>8920
                     /locus_tag="P5079_06555"
     CDS             <1..>8920
                     /locus_tag="P5079_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRY29684.1"
                     /translation="SVVGSLADGGFGATKGIGRALARMLPPGAQIDLVTHSRGGIVGD
                     LMTLDWSDAADAVSGVNHYEVQLSTFETFDLLEYSSVTPASDLPVTGLSNGLHYWRVK
                     AADAAGNATSFASTRSFVVDASTPMVVDLQSGDDTWYLSDPGAAFNVDFEDIGSGLTT
                     AQYTVDESPGLAGGEGDIVPWTDIFTATGIASYAADWSLDFNALPEGPHYVSVRAFDR
                     VHFSTVATDVFYIRKDTSAPSFVDLQSGDTTWRSLSGTAYNVDFQDPGSGLSFIQYSV
                     RLSTEGAGTLVKDWTDIAAPPVNQTTYATDWPVDFVSLQETVTNYVSVRAVDNLGHST
                     TVYDAFFVLKDVTPPTLSDNQLDDVAWHRADPGADYDVDFNDAGGSLLSHVDYTVAGA
                     AGLAGGEGDILPWTNIALNVNTTYYDALWGVDFAALPEGQHYISARAVDFAGNVQVLP
                     DAFFVRKDTTPPSVTVGGGLSGGDTTLRSVNDGTYDVDFADAGGSLLSSFEIKASTMA
                     GGTGPDLVGYTTVLSGISSDTYTTNWSLPSAVFNALLAGDVTNYITVRVFDLAGSSTT
                     QTDAFFVKKDTAAPSFVNNMAGGDALWRRADPGAVYNVDFQIGGIARLDRVQYAAASG
                     PGLPLGDGDLIGWTDIAGPNLDQSSYAADWSVDFAALKSSFNWISVRAISNSASVSTL
                     ADAFIIRKDTESPAVTDLQSGDLVYRNAAGTVYNVDFADALSLLTTAQYTVASSSDLP
                     GGTGNVVPWTDIFTSAGVASYTTDWAVNFSSLHENATNYVSVRVYDVAGSSALMKDAF
                     FVLKDTTPPTYTDNQTGDDTWRRANTGLYAVEFHDTGGGRLSYFQTKITTSPSQSSQL
                     VDDWRTVQSGLATNDLTAAWALAETSFGLLPAGTSYVHVRVYDAAGNVCVPVLVNAFS
                     VRKDTQAPTFVNGEAEGDNTWRADAKTGGYNVDASDVLSAVHTYQFAVSSSPALPGGD
                     GNVIPWTTIAGPALNQPLYSTDWAVNFTALRETVTNYVSVRAWDMAGTTGTLVDAFRI
                     LKDTTPPAVTNHMPAGSDAVWRTANPGAVYNVDFVDTGGSRLSKFQIKVTSGPSQTGT
                     VAADWTDVSSGLAADDFSAEWSLPEAVWSALLQGPTNHVSVRAFDVAGNTSTVSTDAF
                     KVFRDTTTPQITDNQGGDNTWYASDPGAIYDVDFADQESLLTTAEYRLSTSAAQGNPV
                     TSWLAIFTSTGRTLYTDPWGPDFSAAVDGQNWVSVRAQDKAGNPVSQDDVFYLRKDSL
                     SPTVTNNESGGDLTWRKEARAGGYNVDFYDTGAGVAVASYTVYDQTGRTGAQIIPWTT
                     LVDLSGSPQSSYTVDWAVDFALLASGTGYVSVRLADSLGHGTTVNDVFFVRKDTTPPN
                     ALNTVAAAEGSSAGEIVLTWTAPGDDGATGQAASYVVKSKTAQFADQNDFTAANTYSQ
                     SWTPVAQGAGENKTVTGFTEGTTYYFAVEAVDKAGNQAALSNLTGNSAPARRVAPAAI
                     GDLAAVTGDYPGEVTLGWTAVGDDNTSGTAAGYLVKYATYTITAGNFDSVSTYSQSIP
                     PKAAGQTENLLVGGLTAGTTYYFAVKAYDDAQPWANDGGLSNVVSTRAAPSGPAAGMI
                     AYGEGTQAAPRFDEWTGSAWQGSERTAQTAQATLTWSTLKSAAVHRNRKILGTLSSAG
                     NLYVQTWNGDTDTWTSLFNTSNASMSSGYKGYDIAFEQNSGRALIAYYNGTPGRVAYR
                     VWSSTADALVDGPTDFVLGTYSGAVSWVRLVPRPGTNDILLVTKDVNNDVYAARWNGS
                     SFVNGREVTTSGVSAAYPAFDGAWETQSGRALVLYGLGTNLSYDIFSTTQGAWVATGA
                     AGPAIAAGTAVQWVRLASDPISNRIGMTCIDNGSDWNAGVWSGSAWGALPAEDASADS
                     NATRITDLAWEKDTGKLVVAAVDSATPAQAFDYITWQNNTWSANPGSAVADTFNLGNG
                     SDLLWLELIPDPNTNKTILKGISANRTVATRNWTGSGWTDGTHHETNASTTTRECASL
                     ALDMHDTVIPSFVSETPVNADWQKDNSRLYNVDVVDAGGSKLTQIESKIATGAGQTGT
                     VVQDWTTQVSGINADSYVTDWPLTSGTFNLIPENQTVYVSLRVSDAAGNQKTANDLIT
                     LKKDASAPSITDNQSGDDTWRNADPGNIYNVDFNDTGGSNFPATPSGAQYAVASAPGF
                     PGGAGDIISWTNIPAVIGQSFTSDWGLNFSLLSPGTNYVSVRAYDTAGSTTVLPDVFY
                     VKKDTVPPAAVADLAAVPGNVKGQIVLSWTAPGDNGSSGNNGNGSYLVKYSSQGSIND
                     GNFSAAAPWPQTWVPVAAGQQESHVITGLTAGTTYYFALKTYDKVPNVALISNSPSGR
                     PGTDEILINEIYASGGAGQDWVEFYNASSNSKLLDGWTLVYNNGHLSAPGTDSVVWTG
                     TGADSISSGAIRKIALSADIAGTAPHYVVFKDSVPSTVDVVQWPALPAGTALARIYDG
                     HPRWEIDPTPTSDARNAISTGPVKINEADYTALTQEFIELYNTSGSTVSLTDCVLRSK
                     ANVRFVFDRVVMPNGFSAVLYPSVDKLGTSYTDAFGAAGLDSAADFLVLENAAGQVLD
                     RVGWQTASNYVHYDSQAQPASLPDAAAGGIVSPNVLGRKPSEGYDTDLDAADFGTLAS
                     PSLGGRNNTDSLAAANALDYPGDATVLPRRFKVALTLGENSAGGEVDTVWFVRTGGAT
                     DNDSPHIYRLDALGYDRSSTVLQSATFTGTQFTDLDGHPLVNGAVYKMILNSDVASGR
                     APQLLRAGLTYDASVHAVAGSTLSVVSTVNNGTLEPLLKITLLNTGSSGGNSLELERL
                     NVRFKDNGGTDLTTPNAAALFSSLRLVRDSTVGQTGLYEAAFDTATLVDLPDTSFNLV
                     GGGQSLEVTDPNSAQALVAAGETATFFLVAELADDASLQSPNTFRALTDLDADASFRE
                     SVSDVPQTPAAVDAMTTPVFTAVAPPAKM"
BASE COUNT         1622 a         3216 c         2630 g         1452 t
ORIGIN      
        1 gagcgtcgtc gggagcctgg ctgacggcgg gttcggagcg acgaagggga tcggacgggc
       61 gctggcgcgc atgctcccgc ccggcgccca gatcgacctg gtgacccact cgcgcggtgg
      121 catcgtcggc gacctgatga cgctggactg gtcggacgcc gcggacgcgg tttccggcgt
      181 gaaccactat gaggtccaac tttccacgtt tgaaacgttc gatctcctcg aatacagttc
      241 cgtcactccg gcctccgacc tccccgtgac cggattaagc aacggtctcc attattggcg
      301 cgtgaaggcg gcggacgcgg ccggcaacgc cacctctttc gcctcgacgc gcagtttcgt
      361 ggtggacgcc tccacgccca tggtcgtcga ccttcaatcc ggcgacgaca cctggtatct
      421 ctccgacccc ggggcggcgt tcaacgtgga ctttgaggac atcggctcgg gcctgaccac
      481 cgcccaatat accgtcgacg aatcccccgg actggccggc ggggagggag acatcgttcc
      541 ctggacagac atctttaccg ccacgggcat cgcctcttac gccgccgact ggtctctgga
      601 cttcaacgcc ctgcccgaag gtccgcatta cgtgtcggtc cgcgctttcg accgcgtcca
      661 tttctccacg gtggcgaccg acgtctttta catccggaaa gacacctcgg ccccgagctt
      721 cgtcgacctc cagtccgggg acaccacctg gcggagcctt tccggaacgg cctacaacgt
      781 ggatttccag gaccccggtt ccggactgag tttcatccaa tattccgtcc gtctctccac
      841 cgaaggcgcc ggcacgctgg tgaaagactg gacggacatc gccgcgccgc ccgtgaacca
      901 aacgacctac gcaaccgact ggccggtgga tttcgtttcg cttcaggaga ccgtcaccaa
      961 ctacgtatcg gtgcgggccg tggacaacct ggggcacagc accacggtgt atgacgcctt
     1021 cttcgtactg aaagacgtga ccccgcccac cctctccgac aatcaattgg acgacgtcgc
     1081 ctggcaccgc gccgaccccg gggcggacta cgacgtggac ttcaacgatg ccggcggctc
     1141 gcttctgtcg cacgtggact acaccgtcgc cggcgccgcc ggcctggccg ggggagaggg
     1201 ggacatcctt ccgtggacga acatcgctct gaacgtcaac accacctact acgacgccct
     1261 gtggggcgtc gatttcgccg ctcttccgga aggccagcac tacatctccg cgcgcgcggt
     1321 ggacttcgcc ggcaacgtcc aggtcctgcc ggacgccttc ttcgtcagaa aagacaccac
     1381 cccgccctcc gtcaccgtcg gcggaggcct ttccggcgga gacaccacgc tccgcagcgt
     1441 caacgacggg acctacgacg tggacttcgc cgacgccggc gggagcctgc tgtcctcttt
     1501 cgaaataaaa gcgtccacga tggccggcgg gaccggcccg gacctggtcg ggtataccac
     1561 cgtcctcagc ggcatttcgt cggacacgta cacgaccaac tggtccctgc cttccgcggt
     1621 gttcaacgcg ctgctggccg gagacgtcac caactacatc accgtccgcg tctttgacct
     1681 ggccggttcc agcacgacgc agaccgacgc cttcttcgtg aaaaaagaca ccgccgcccc
     1741 gtcgttcgtc aacaacatgg ccggagggga cgccctttgg cggcgggcgg accccggcgc
     1801 cgtctacaac gtggacttcc agatcggcgg catcgcccgg ctcgaccgcg tccagtacgc
     1861 cgccgcctcc ggccccggac ttccgctcgg agacggcgac ctcatcggct ggaccgacat
     1921 cgccggaccg aacctcgacc agtcctccta cgccgccgac tggtccgtgg atttcgcggc
     1981 gctcaaatcg tctttcaact ggatcagcgt ccgagcaatc tcgaactccg cttccgtcag
     2041 caccttggcg gacgccttca tcatccgcaa agacacggag tcgccggcgg tcaccgacct
     2101 gcagagcggc gacctcgtct accgcaacgc ggccggaacc gtttacaacg tggactttgc
     2161 ggacgcgctt tccctcctca ccaccgcgca atacaccgtg gcctcctctt cggacctgcc
     2221 gggcgggacg gggaacgtcg tcccctggac ggacatcttc acgtcggccg gggtggcgtc
     2281 ctacaccacc gactgggcgg tcaacttctc ttccctgcat gaaaatgcca ccaactatgt
     2341 ctccgtgcgt gtctacgacg tggccggctc ctccgcgctc atgaaagacg ccttcttcgt
     2401 tttgaaggac accaccccac ccacctacac cgacaaccag acgggagacg acacctggcg
     2461 gcgcgccaac acggggctct acgccgtcga gttccacgac accggcggag gacggttgtc
     2521 ctatttccaa acaaagatca ccacctcccc gtcgcagagc agccaattgg tggacgattg
     2581 gcgcaccgtg cagtccggtc tggccacgaa cgatctcacc gccgcctggg cgctggcgga
     2641 aaccagcttc ggcctccttc ccgccgggac cagttacgtg cacgtccgcg tctacgacgc
     2701 cgccgggaac gtgtgcgtgc cggtgctggt caacgccttc tccgtccgca aagacaccca
     2761 ggcgccgacg ttcgtcaacg gggaagcgga gggagacaac acgtggcgcg ccgacgccaa
     2821 aaccggggga tacaacgtgg atgccagcga tgtcctgtcg gcggtgcaca cctatcaatt
     2881 cgccgtctct tcctctcccg ccctgccggg aggggacggg aacgtgatcc cctggacgac
     2941 gatcgccggc cccgcgctca accagcccct ttattccacc gactgggccg tcaatttcac
     3001 cgccctgcgg gaaacggtca ccaattacgt ttccgtccgc gcctgggaca tggccggcac
     3061 caccggcacg ctggtggacg ctttccggat actgaaagac accacgccgc cggcggtgac
     3121 caaccacatg cccgccgggt cggacgccgt ctggcgcacc gccaaccccg gcgccgtcta
     3181 caacgtggat ttcgtcgaca ccggcggcag ccgcctttcc aaatttcaga tcaaggtcac
     3241 ctccggaccc tcccaaaccg gcaccgtggc ggcggactgg acggacgtct ccagcggttt
     3301 ggcggccgac gatttttcgg ccgaatggtc cctgcccgag gccgtctgga gcgcgctcct
     3361 gcaagggccc accaaccatg tctccgtgcg ggccttcgac gtggccggca acacctccac
     3421 ggtctccacg gatgccttca aggtcttccg cgacacgacc acgccccaga tcaccgacaa
     3481 ccaagggggg gacaacacct ggtatgcctc ggacccggga gccatttatg acgtggattt
     3541 cgcggaccag gaatcgctgt tgacgacggc ggaataccgc ttgtccacct cggccgccca
     3601 ggggaacccg gtgacgagct ggctggcgat tttcacctcc acgggaagaa ccctttatac
     3661 ggacccctgg ggcccggact tctcggccgc cgtcgacggc cagaactggg tctccgtgcg
     3721 cgcgcaggac aaggccggca atcccgtctc gcaggacgac gtcttctacc tgaggaaaga
     3781 ctccctctcc ccgacggtga cgaacaacga atccggcggc gacctgacgt ggcgcaagga
     3841 ggcgcgggcc ggcggttaca acgtggactt ctacgacacc ggcgcggggg tggccgtcgc
     3901 ctcctatacg gtgtatgacc agaccgggcg gaccggcgca caaattattc cgtggaccac
     3961 cctcgtcgac ctcagcggct cgccgcagtc ctcctacacg gtggactggg ccgtggactt
     4021 cgccctcctc gccagcggca ccggctatgt ctcggtgcgc ctggcggact cgctcgggca
     4081 cggcacgacg gtcaacgacg tctttttcgt tcggaaggac accacgccgc ccaacgcgct
     4141 caacaccgtg gccgcggccg agggctcttc cgccggtgaa atcgttctga cctggaccgc
     4201 ccccggcgac gacggcgcca ccgggcaggc cgcctcctac gtcgtgaaat ccaagacggc
     4261 ccaattcgcc gaccaaaacg acttcaccgc cgccaacacc tattcgcaat cctggacgcc
     4321 ggtcgcgcag ggggcggggg agaacaaaac cgtcacgggg ttcaccgaag gaacgaccta
     4381 ttatttcgcc gtcgaggcgg tggacaaggc gggcaaccag gccgcgctga gcaatctgac
     4441 gggcaactcg gcgccggcac gtcgtgtggc gccggccgcc atcggcgact tggcggcggt
     4501 gaccggcgac tatccgggcg aggtgaccct gggatggacc gccgtcggcg acgacaacac
     4561 cagcggcacc gccgccgggt acctcgtgaa atacgccacg tacaccatca ccgcggggaa
     4621 tttcgattcc gtctccacct attcccaatc catccccccg aaagcggccg gccaaacgga
     4681 aaatctgttg gtgggcggac tcaccgccgg aaccacctat tatttcgcgg tcaaggccta
     4741 tgacgacgcc cagccgtggg ccaacgacgg gggcctctcc aacgtggtct ccacgcgcgc
     4801 cgccccttcc gggccggcgg ccggcatgat cgcctacggc gaaggcacgc aggccgcgcc
     4861 gcgcttcgac gaatggacgg ggtccgcctg gcaggggtcg gaacgcaccg ctcagaccgc
     4921 ccaggccacc ctcacctgga gcaccctcaa atcggccgcc gtccaccgca accggaagat
     4981 cctcggcacc ctctcttccg ccggcaacct ctacgtgcag acctggaacg gcgacacgga
     5041 tacctggacc agcctcttca acaccagcaa cgcctcgatg agttcgggat acaaaggcta
     5101 cgacatcgcc ttcgaacaga attccggccg ggcgctgatc gcctattaca acggcacccc
     5161 gggccgggtg gcctaccgcg tgtggtcctc cacggccgac gccctggtgg atggcccgac
     5221 ggatttcgtt ctcggcacct actccggcgc ggtcagctgg gtgcggctcg tccctcggcc
     5281 ggggaccaac gacatcctgc tggtcaccaa ggacgtcaac aacgacgtgt acgccgcccg
     5341 atggaacggc tcctccttcg tgaacggccg ggaagtgacg acctccggcg tgtcggcggc
     5401 gtacccggct tttgacggcg cctgggagac gcagagcggc cgcgccctgg tgttgtacgg
     5461 actgggcacc aatttaagct atgacatttt ctcgaccacc caaggggcgt gggtcgccac
     5521 gggcgccgcg ggacccgcca tcgccgccgg aaccgccgtc caatgggtgc gtctggccag
     5581 cgaccccatc tccaaccgca tcggcatgac ctgcatcgac aacggctccg actggaacgc
     5641 cggcgtctgg tcgggcagcg cctggggcgc tctgccggca gaagacgcct cggcggactc
     5701 caacgccacc cgcatcaccg atctggcgtg ggagaaagac accggcaaac tggtcgtcgc
     5761 cgcggtggat tccgccaccc ccgcccaggc gttcgactac atcacctggc agaacaacac
     5821 ctggtcggcg aaccccggct ccgcggtggc cgacaccttc aacctcggca acggctcgga
     5881 cctcctgtgg ctggaactca tccccgaccc caacaccaac aagacgatcc tgaaaggcat
     5941 ctccgccaac cgcaccgtgg ccacgcgcaa ctggacgggc agcggctgga cggacggcac
     6001 ccaccacgag accaacgcct ccaccaccac gcgcgaatgc gcctccctgg ccctggacat
     6061 gcacgacacc gtgataccga gtttcgtgtc ggaaaccccc gtcaacgccg actggcaaaa
     6121 agacaacagc cggctttaca acgtggacgt ggtggacgcg ggcggatcga agctgacgca
     6181 aatcgaatcg aagatcgcca ccggcgccgg acagacgggc accgtggtcc aggactggac
     6241 gacgcaggtc tccggcatca acgccgattc ctacgtcacg gactggcctt tgacctccgg
     6301 aacgttcaac ctcatccccg aaaaccagac ggtgtatgtc tcgctgcgcg tctccgacgc
     6361 cgccggcaat caaaaaaccg ccaacgacct tatcactctg aagaaggatg cctcggcgcc
     6421 gtccatcacg gacaaccagt ccggggacga cacctggagg aacgccgatc ccggaaacat
     6481 ttacaacgtt gatttcaacg ataccggcgg ttccaatttc ccagccacac cgtccggcgc
     6541 ccaatacgcc gtggcctccg ccccgggctt cccgggagga gccggtgaca ttatttcctg
     6601 gacgaacatc ccagccgtca tcggtcagtc cttcacgagc gattggggac ttaacttttc
     6661 gcttctatcg ccgggaacca actatgtctc cgtgcgcgcc tacgacaccg ccggctcaac
     6721 caccgtgttg ccggatgtct tctacgtgaa gaaggacacc gtcccgccgg cggcggtggc
     6781 cgatctcgcg gccgttccag gcaacgtgaa agggcagatc gtcctctcct ggacggcccc
     6841 cggagacaac gggtcttccg gcaacaacgg gaacggctcc tatctggtga agtattcttc
     6901 ccaggggtcc atcaacgacg ggaacttctc cgccgccgcc ccctggccgc agacgtgggt
     6961 ccctgtcgcc gccggccaac aggagtccca cgtcatcacc ggattgaccg cggggacgac
     7021 gtactatttc gccctcaaaa cctacgacaa agtccccaac gtcgctctca tttccaattc
     7081 gccctccggc cggccgggga cggacgagat cctcatcaac gaaatttatg cctccggcgg
     7141 cgccgggcag gactgggtcg agttctacaa cgccagttcg aattccaaac tcctcgacgg
     7201 ctggacgctg gtgtataaca acggccatct ctccgccccg ggaacggatt cggtggtctg
     7261 gaccgggaca ggggccgatt ccatctccag cggcgccatc cggaaaatcg ccttgagcgc
     7321 cgacatcgcc gggaccgccc cgcattacgt ggtgttcaag gattccgttc cctccacggt
     7381 ggacgtggtg caatggccgg cgctgccggc cggcaccgcc ctggcccgga tctacgacgg
     7441 ccacccccgc tgggagatcg accccacacc cacctccgac gcgcggaacg ccatcagcac
     7501 cgggccggtg aagatcaacg aggcggacta caccgcgctc acccaggaat tcatcgagct
     7561 ttacaacacg tcgggttcca ccgtttcgtt gacggattgc gttctgcggt caaaagcgaa
     7621 cgtgcgattt gtgttcgatc gggtggtgat gccgaacggc ttctccgccg tgctgtatcc
     7681 gtccgtggac aaactgggaa cttcgtatac ggacgccttc ggcgccgccg ggctggacag
     7741 cgcggcggac ttcctcgtgc tggaaaacgc cgccgggcag gtcctggacc gcgtgggctg
     7801 gcagaccgcc tccaattacg tgcactacga ctcccaggcg caaccggctt ccttgcccga
     7861 tgccgccgcg ggagggatcg tcagccccaa cgtcctgggc cgaaaaccgt ccgaaggata
     7921 cgacaccgac ctggacgcgg cggatttcgg cacgctcgcg tccccgagcc tcggcggacg
     7981 caacaacacc gattccctcg ccgccgccaa cgctctggac tatcccggcg atgccacggt
     8041 cctgccgcgg cggttcaagg tcgccctgac cctcggcgaa aattccgccg gcggcgaggt
     8101 ggacaccgtc tggttcgtcc gcacgggggg ggcgacggac aacgattccc cgcacatcta
     8161 ccgtctcgac gcgctcggct acgaccgttc ctcgacggtc ctgcaaagcg ccacgttcac
     8221 cggaacgcag ttcacggacc tggacggcca tccgctggtg aacggcgcgg tgtacaagat
     8281 gatattgaac tccgacgtgg cttccggccg ggccccccaa ctgctccggg ccggcctcac
     8341 ttacgacgcc agcgtccatg cggtggcggg gtccaccctg tcggtcgtgt ccaccgtcaa
     8401 caacggcacg ctggaaccgt tgctcaaaat caccctgctc aataccggct cttccggagg
     8461 caactccctc gaactggaac ggctcaacgt ccggttcaag gacaacggcg gcacggacct
     8521 gaccactccg aacgcggccg ccctgttctc ctcgctgcgt ctggtgcggg attccacggt
     8581 cggccagacg ggcctctatg aggccgcctt cgacaccgcc actcttgtcg acctgccgga
     8641 cacctcgttc aacctggtcg gcggcgggca atccctggag gtgacggacc cgaactccgc
     8701 ccaggccctc gtggcggcgg gcgaaacggc cacgttcttc ctggtcgcgg aactggcgga
     8761 cgacgccagc ctgcagtccc cgaacacctt ccgggcgttg acggacctgg acgccgacgc
     8821 gtctttccgc gaatccgttt ccgacgtccc tcagacgccc gccgccgtgg acgcgatgac
     8881 gacgccggtg ttcaccgcgg tggcgccgcc ggcgaagatg
//
LOCUS       DAOTFD010000093         8804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5642_length_8804_cov_4.239799, whole genome shotgun sequence.
ACCESSION   DAOTFD010000093 DAOTFD010000000
VERSION     DAOTFD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5642_length_8804_cov_4.239799"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>8804)
                     /locus_tag="P5079_06560"
     CDS             complement(<1..>8804)
                     /locus_tag="P5079_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRY29685.1"
                     /translation="VLRESDIPATDQHLYNVDDWATLLDGLFKTYRFTADDKTMDRLR
                     EILPHVYAELKNLNEPDDLPNVLSTYIEPVTGRLFPYHRELMFDPEIGYRADRNLMKK
                     VSLAVFYALELQDRMDRAEAKGQPLTQQEKDAWLKSRMDSDLRLFAVAAREHRGEPVD
                     RADQEAYLRLLRSRTATWNRFMFRHLTSSEKAAYRKAAREVVERFFPGLSSLADINAG
                     LQSGVMVDGRPQEISELGDLKQESLYRSLMAFAVARNMTPRQMVEYMGMVQRTFLPEV
                     ERYLKDAGMSELAQLPDSDRLRFLNKWIHELQAKQARADVSPADKAELDQTIRKLRNI
                     IEIELQNERSNMVFATTLFWADQFMRDRGYRDPRAALTDEDRARLGANFKDFIDKLVQ
                     LRESPVAEKYVRMQLQIPQNVAINWLSTDAQGMLSAITLDWMKEKWTMAEVTAHLNNV
                     IYAAENLTDEFEKYAAAVGLPQSFDTHVSDPLERVRVWGELAARTRMLEKVLTLEIEN
                     GALRRNGRKVGSLADITGEDLSYVMDKLNRVYDAAQKNGISLEDGEGQYFYELLHGLR
                     QDMDFVSLDLGINGEIERFFADYAAAKRASRNAGAALLREYATQANLPEWMSPAERKL
                     FFYYITGHADGLPNREGLMHRKVMDGDAVFKAAKYMRLRGFTEQDLVAEEKAKMYLQA
                     IYMLLHTQDGVPRELSKSDLSSYLNMVTRGLNSLEEIGLLFKYDSISEGMEEQNAAAL
                     GGQMPRLSKAQRFGLGSRMLQLGQGANEIRAWFRDMFKIQRAAREGLGDFLAPQALSA
                     LIEPFRRNKPELNDYLEGYTRDRFNSTAREDVFLMDLGEEAGLLRRVLLTHLTTEGEL
                     PSKERTNQIMEWWRDLEGRLRKERNLDAGAVVAYQQEFAELMAQAKQTFMDLLAPAMY
                     ERNPQGNLHDDLQKEVARMEEFLAPRLIDYLVRYEARQKAVKLLLDESARERSVLEPI
                     QDRLKAERAARVERYRAELQNEAAKKTALLELDRLARTSYGMNLDEGEKAYYLKKMKE
                     LGYTPEEVLFRFIHLKDLVAQTMYGRTELTEQEKGVVAAFADDIFDRFGFMSAGDRQA
                     MEKGLGLTRDEAVARDVAKIRRQLDYAVKIRGLYREMGLPISESKSRSLAADAIAGAV
                     ELPDIRQWLEFANLVRDIGEAHGLPRDRDTLDDLLFSSDIFKLGLADLPTKEIVRTPI
                     DEKQLEAQIVAEMARMGIELTPYQLPALVQRMKERRISPVALRMYGEKMQRVERMMEE
                     ARRADAEKSVVDDMTKAPRGYYDLNLGMRKKWVLALLDHLNPNTKTHIGIVDILGPGY
                     DAKILEKRMGRPIFAMYSQMVRDELMRRLPESLWQRNWSEFEKWRYNAWKQKGWGNIQ
                     NIMALGAIAGTVKFAFLLFQKFFGHRKMSKEQRKELTLSWSEVLFFTMRTVITATLLT
                     GGVVLSLNFSYFNAARLTLFVVVTWYMTTIVADKYILLPIIWIGAHVFEPFKKLQTKI
                     TGKESSFIHLHRRKDIMEAGRIPDSTPLKLILSDFVSFDKEEPSMIKKNSRIEYDFIL
                     QRQTIPLLIEDYLRAKKAGGQQNLYYNMVLQSRGSGPRGDATDKEHEIIKHFLKERLE
                     ELLTAKELSVILRRDVSEEEAAELAAAVKRRTYIFLRRTPVKKPFMQYVVHRWFMETD
                     EEYQNVTDPLEQELNVDPVNNPDGTVDKSKRVPMPLEDVLFGDPSDPRDLRNLRQRNI
                     ASHPDPMHREREGEFDLTLTYDMGNEVDYDGLLRMAATAADERNEAYMYRCKMVFNDV
                     YSSLHTFIMKYFPLDMLNFMMEAQQYLFKSVRAPGKYMYRDRDYYRKLLKTQAYEKIW
                     PFYTRLKSTNDVLPKSEDEVHTILAGKMAYVGDVDMSEDPQYFYLQEAARDETKWSKI
                     FDWPQLFVFRLAPSVRDHPWMTAFKVGWYPAFAIAGLLGASMTYFVWIPILVGLASAL
                     TVGLFLFKPASRKLRAMEASGKMWIPRMSPGHMYESNMVLRGQITEPIWFLQLAVTFI
                     AMLRPGNIEVHFPFLGEILFTFLMILLTMPKLFPSIAALLFGKKLPGYDEKDPEANKP
                     RKIDWLLRIPRAIGYVLLGNLEFSASQSYTTKVVRNPFIKIMQIKDTAKIVYYHIFRP
                     NDPPPPAPWIPASLGDKSPNYLKYLWFFRWPFAVGLAVVMITILPLLTSYGTIRLIGM
                     GGALVLAAAFHIPRAFDPKLKSSPLLNRVGTAVSSALIAVLLAYVGLHAQYLNTVLPF
                     DSMLRAATIFVSSFLFAPARTWLEGKEWRDMFGEKWGRRAVRWGAYTLGFVVLASSIL
                     GIFFPTDCATWVSAVFAGPIPMGDGSASLYAWWLPMQENLRLPWFFVVFFTSILVSSV
                     VIGVVATVAGVISGIAVGLRNVLSYVLSGDIRHELAKLSKGNLNVLDDIFAYAFMSPA
                     NRKKKRDFLQEEAKKKQAAGELRQTIDRHLSSKGQPEKAEPPASPAETSAPLVGKKAD
                     EQTGTKVETPEEEPKKTTTEAPSAGKPSPAVGKKSGEPVGAKVEEPEKEEPKKETTAV
                     VSAADKPVAAQALPLVTPHPDADKKPAGSLAKKIYLALGALVLAAAMMVSAWLGRPTG
                     ENRNPMPLPPQQTIETTAPAPRAPVLPPAAKPSVSAEQQARPPAAPVVPTAVAVPQDT
                     AASLRAERDNFLSRAKDQVLLEKMEESALSDYEDFIQAAARENGLDPDVIRAIILQES
                     RGKAGLVSKRGAHGLMQLLSRFFGGQDLKEPKRNIEKGSAFYRSLLATYNNGRRTPLA
                     AEELAWAAYNAGPTTVNLLLEATGDPRSATLQDIFDDLPLETQRHQVGILHFLVQLKG
                     GAADIPSELDAKVMKPGQAKKSAPKASPNRFVRAKGLFATGLVSLTAGAASMSLMAGA
                     AVWLGAGVWAPFALLVPLSLALSALARSHRTRTAALMLSGVYPVLVGG"
BASE COUNT         1590 a         2702 c         2717 g         1795 t
ORIGIN      
        1 gcctcccacc aagaccgggt aaacgcccga caacatcagc gccgcggtcc gcgtccgatg
       61 ggaacgcgcc aacgcgctga gcgccaggga gagcgggacc aggagagcga agggcgccca
      121 cacaccggcg ccgagccaca ccgccgcccc ggccatcaaa gacatcgacg ccgcgccggc
      181 ggtcagcgag accaagcccg tcgcgaacaa gcctttcgcc cgaacgaagc ggttgggcga
      241 ggccttggga gccgatttct tcgcctgacc cggtttcatc accttggcgt ccagctcgga
      301 cgggatatcc gccgccccgc ctttcagctg aaccaggaaa tgcaggatcc cgacctgatg
      361 cctctgcgtt tccaacggga ggtcgtcgaa gatgtcctgc aacgtggcgg accgcggatc
      421 gcccgtggcc tccaagagga ggttgacggt ggtggggccg gcgttgtaag cggcccacgc
      481 cagctcctcg gcggccagcg gcgtgcggcg gccgttgtta taggtggcca acaagctccg
      541 atagaaggcg gagcctttct cgatattcct cttcggttcc ttcagatctt gtccgccgaa
      601 gaacctggac agcaactgca tcaggccatg cgcgcctctc ttcgaaacga ggccggcctt
      661 gccgcgcgat tcctgcagga tgatggcccg gatcacatcc ggatccagac cgttctcccg
      721 ggccgcggct tgaatgaaat cctcgtaatc cgacagggcg ctttcctcca ttttttcgag
      781 gaggacctgg tccttggccc gggacaggaa attgtcccgt tcggcccgca gggacgccgc
      841 ggtgtcctgt ggaacggcga ccgcggtggg aacaacgggg gccgccggcg gccgcgcctg
      901 ttgctcggcg gaaacggacg gtttcgccgc cgggggaagg accggggctc gcggggccgg
      961 cgcggtcgtc tcgatggtct gttgcggcgg cagaggcatg ggattccggt tttcgcccgt
     1021 cgggcggccc agccaggcgg aaaccatcat ggcggccgcc aacacaaggg cgccgagggc
     1081 caggtaaatc ttcttggcga ggctcccggc cggtttcttg tcggcatcgg gatgcggcgt
     1141 cacaagggga agcgcctgtg ccgcgaccgg cttatccgct gcggaaacaa ccgccgttgt
     1201 ctccttcttg ggctcttcct tttccggttc ctcgaccttc gcgccgaccg gttcgcccga
     1261 ttttttaccg accgccggcg acggttttcc cgccgagggt gcctccgtcg ttgtcttctt
     1321 gggttcttcc tccggcgttt cgactttcgt tccggtctgt tcgtccgcct tttttccgac
     1381 caacggcgcc gaagtctcgg cgggcgatgc ggggggctcg gctttttccg gctgtccttt
     1441 cgaactcaag tgtcgatcta tcgtctgacg caactcgccg gcggcctgct ttttctttgc
     1501 ttcctcctgc aaaaagtcgc gtttcttttt ccggttggcg ggcgacatga acgcgtaggc
     1561 aaaaatgtcg tcaagcacgt tcaggttccc cttggagagt ttggccagtt cgtggcggat
     1621 atcgccgctc aacacatagg ataacacgtt ccgcagcccc accgcgatgc cactgatgac
     1681 gcccgcgacg gtggccacca cgccgatgac gacgctgctg accaggatgg aggtgaagaa
     1741 gaccacgaag aaccagggga gccgcaggtt ctcctgcatg ggcagccacc aggcgtacag
     1801 actggccgac ccgtcgccca tggggatggg cccggcgaac acggcggaga cccaggtggc
     1861 gcaatccgtc gggaagaaga tgcccaaaat gctggaggcc aggacgacga accccagcgt
     1921 atacgccccc caccgcacgg cgcggcggcc ccatttctca ccgaacatgt cccgccattc
     1981 cttcccctcc agccaggtgc gggccggcgc gaaaaggaag gaggaaacga aaatggtcgc
     2041 ggcgcggagc atggagtcga acggcagcac ggtgttgaga tattgagcat ggagaccgac
     2101 ataggccaac aagacggcga tcaacgcgct ggacacggcc gtgccgaccc tgttcagcag
     2161 tggactgctt ttcagtttgg gatcgaacgc ccgcgggatg tggaaagccg ccgccaacac
     2221 cagcgcgccg cccatgccga tcagccggat ggtcccgtaa ctggtcaaca gcgggaggat
     2281 ggtgatcatc accaccgcca gaccgacggc gaacggccat cggaaaaacc agaggtattt
     2341 aaggtagttg gggctcttgt cgcccaggct ggccgggatc cacggcgccg gcggcggcgg
     2401 atcgttcggc cggaagatgt ggtaatagac gatcttcgcg gtgtccttga tctgcatgat
     2461 tttgatgaag gggttccgca ccactttcgt ggtgtaggac tgggatgcac tgaattccag
     2521 gttccccaac agtacgtacc cgatggcccg gggaatgcgc aacaaccagt cgattttcct
     2581 gggtttattg gcctcggggt ccttctcgtc gtagccgggc agctttttcc cgaacaacaa
     2641 agcggcgatg ctcgggaaaa gtttcggcat ggtcagaagg atcatcagga acgtaaagag
     2701 gatctcgccc aggaacggga agtgcacctc gatgttgccg ggcctgagca tggcgatgaa
     2761 ggtcacggcc agttgcagga accaaatggg ctcggtgatc tgcccgcgca ggaccatgtt
     2821 ggactcatac atgtgaccgg gagacatgcg cgggatccac atcttccccg acgcctccat
     2881 ggcgcgaagt ttccgactgg ccggtttgaa caggaacagc ccgacggtca gggccgaggc
     2941 aagaccgacc agaatcggaa tccagacgaa ataggtcatg gaggcgccca agagccccgc
     3001 gatggcaaaa gccggatacc aacccacctt gaacgccgtc atccacgggt ggtcccggac
     3061 ggacggagcc agccggaaca cgaacaactg gggccagtcg aagatcttcg accatttggt
     3121 ctcgtctcgg gcggcctcct ggagataaaa atactgcggg tcttccgaca tgtccacgtc
     3181 gcccacgtag gccatttttc ccgccagaat ggtatggacc tcgtcttcac tcttcggcag
     3241 aacatcgttg gtcgatttca gccgagtgta gaaaggccag atcttctcat acgcctgcgt
     3301 tttgagaagt tttcgataat agtcccggtc gcggtacatg tatttgccgg gagcgcgcac
     3361 ggacttaaag agatattgct gggcctccat catgaaattg agcatgtcca acgggaaata
     3421 cttcatgatg aacgtatgca gggaggagta gacgtcgttg aacaccatct tgcaccggta
     3481 catgtaggct tcgttgcgtt catccgcggc ggtggcggcc atgcgcaaaa gtccgtcgta
     3541 gtccacctcg ttccccatgt cgtaggtgag cgtgaggtcg aactccccct cccgctcgcg
     3601 gtgcatcggg tccggatgag aagcgatgtt ccgctgacgc aagttcctca ggtcgcgcgg
     3661 gtcggagggg tcgccgaaga ggacgtcttc gaggggcatg ggcacccgct tcgatttgtc
     3721 cacggtgccg tcgggattat tgacggggtc cacgttcaat tcctgttcca ggggatcggt
     3781 cacgttctgg tattcctcgt ccgtctccat gaaccagcgg tggaccacgt attgcatgaa
     3841 gggcttcttg acgggggtgc gccgcaggaa gatgtaggtg cgccgcttga cggccgcggc
     3901 cagctccgcc gcctcctcct cgctcacgtc gcgccgcagg atcacgctca actctttggc
     3961 ggtgagcagt tcttcgaggc gctccttcaa gaaatgcttg atgatctcat gctccttatc
     4021 ggtggcgtcg ccccggggcc cggaaccgcg gctctggagg accatgttgt agtaaaggtt
     4081 ctgctggccg ccggcctttt tggcgcggag gtaatcctcg atcaggagcg ggatggtctg
     4141 acgttgaagg atgaaatcgt attcgatccg cgaattcttc tttatcatcg acggctcttc
     4201 cttgtcgaaa gacacgaagt cgctcaggat aagcttgagc ggcgtggaat ccgggatccg
     4261 gccggcttcc atgatgtcct tccggcggtg caagtggatg aaggacgact ccttgccggt
     4321 gatcttggtc tgcagttttt tgaagggctc gaacacgtgg gccccgatcc agatgatggg
     4381 gagaaggatg tatttatccg ccacgatggt ggtcatgtac cacgtcacca caacgaacag
     4441 ggtcaggcgg gcggcgttga aatacgagaa gttcagcgac agcaccacgc cgcccgtcag
     4501 caacgtggcg gtgatgaccg tccgcatcgt gaagaagagc acctccgacc aggagagggt
     4561 cagctctttg cgctgttcct tgctcatctt ccgatggccg aagaattttt ggaaaaggag
     4621 gaaggcgaat ttcacggtgc cggcgatggc gcccagcgcc atgatgttct ggatgttccc
     4681 ccaccccttc tgtttccacg cgttgtagcg ccatttttcg aactcggacc agttcctctg
     4741 ccagagggat tccgggaggc gcctcatcaa ttcgtcgcgg accatctggc tgtacatggc
     4801 gaagatgggc cggcccatgc gtttttcaag gatcttggcg tcgtacccgg ggcccaagat
     4861 gtcgacgatg ccgatgtgcg tcttggtgtt ggggttgagg tgatccaaga gcgccagaac
     4921 ccatttcttc cgcatcccca ggttcaagtc gtaataaccg cgcggggcct tggtcatatc
     4981 gtcgacgacg gatttctccg cgtcggcgcg gcgggcctcc tccatcatcc gctccacgcg
     5041 ctgcatcttt tcgccgtaca tccgcaacgc cacggggctg atgcggcgtt ccttcatccg
     5101 ctgaaccagg gccggcagct gatacggcgt cagctcgatg cccatccggg ccatttcggc
     5161 gacgatctgc gcctccagtt gtttctcgtc gatgggcgtc cgcacgatct ccttggtcgg
     5221 caggtcggcc aggcccagct tgaaaatatc ggaagagaag agcaggtcgt ccagcgtgtc
     5281 ccggtcccgc ggcaggccgt gggcctcgcc gatatcccgg accaggttcg cgaactccag
     5341 ccattgacgg atgtccggca actccaccgc gccggcgatg gcgtcggccg ccagggagcg
     5401 ggacttggat tcggaaatgg gcagccccat ctcgcggtaa aggcctcgga tcttcacggc
     5461 ataatcaagc tgtcggcgga tcttggccac gtcccttgcc accgcctcgt cccgggtcag
     5521 gcccaacccc ttctccatcg cctggcggtc accggcgctc atgaaaccga accggtcgaa
     5581 aatgtcgtcc gcgaacgcgg ccaccacgcc tttttcctgt tccgtcagtt ccgtccggcc
     5641 gtacatggtc tgggccacga gatccttgag gtggatgaac cggaacagca cctcctccgg
     5701 cgtatagccg agttccttca tttttttgag gtaataggcc ttttctccct cgtccaggtt
     5761 catcccgtag ctcgtccgcg ccaaccggtc caattccagc aaagcggtct ttttggcggc
     5821 ctcgttctga agttccgccc ggtagcgttc cacgcgcgcc gcgcgttccg ctttcagccg
     5881 gtcctggatc ggttcaagga ccgacctctc ccgggccgac tcgtccaaca ggagcttgac
     5941 ggctttctgg cgcgcctcgt aacggacgag gtaatcgatg aggcgcggag ccaggaactc
     6001 ctccatgcgc gccacttctt tctgcaagtc gtcatgcagg ttcccctgcg ggttccgctc
     6061 gtacatggcc ggggccagca agtccatgaa ggtctgcttc gcctgcgcca tcaactcggc
     6121 gaactcctgc tgataggcga cgacggcgcc ggcgtccaga ttccgctcct tccgcagacg
     6181 tccttccagg tccctccacc attccatgat ctgattggtc cgttctttgg agggcagttc
     6241 cccctccgtg gtgaggtggg tcagaaggac gcggcggagg aggccggcct cctcgcccag
     6301 gtccataagg aagacgtctt cgcgggcggt ggagttgaaa cggtcgcggg tatacccctc
     6361 gagataatcg ttcagttccg gtttgttccg acggaaaggt tcgatgaggg cactgagggc
     6421 ctgcggggcc agaaaatcgc cgagcccttc gcgggccgcc cgctggatct tgaacatgtc
     6481 gcggaaccac gcccggatct cgttggcccc ctggcccaac tggagcatgc gggagcccaa
     6541 accgaatcgc tgcgccttgc tcaacctcgg catctggccg cccaacgccg cggcgttctg
     6601 ttcctccatc ccctcgctga tgctgtcgta tttgaacagc agtccgatct cctcgaggga
     6661 gttcaggccg cgggtcacca tgttcagata ggaggaaagg tcgctcttgg agagttcccg
     6721 cggaacgccg tcctgggtat gcaggagcat atagatggcc tggaggtaca tcttggcttt
     6781 ctcctcggcc accaagtcct gttcggtgaa cccgcggagc cgcatgtatt tggcggcctt
     6841 gaagaccgcg tcgccgtcca tcaccttgcg gtgcatcagc ccttcgcggt tcggcaagcc
     6901 gtccgcatga ccggtgatgt aatagaagaa gagtttgcgt tcggccggcg acatccattc
     6961 gggcaggttg gcctgggtcg cgtattccct caggagcgcc gcccccgcgt tgcgcgacgc
     7021 gcgtttggcc gcggcgtaat ccgcgaagaa gcgttcgatc tccccgttga tgcccaggtc
     7081 cagggaaacg aagtccatgt cctgccgcag accgtggagc agttcataga aatattgtcc
     7141 ttcgccgtct tccagagaga tcccgttctt ctgagcggcg tcgtagacgc ggttcagttt
     7201 gtccatcaca taggacaggt cctccccggt gatgtcggcg agggagccca ctttccggcc
     7261 gttgcggcgc agggccccgt tctcgatctc cagggtgagg actttttcga gcatgcgcgt
     7321 gcgagcggcc agttcgcccc acacccggac gcgttccagc ggatcggaaa catgggtatc
     7381 gaacgactgg ggcaggccga cggccgcggc gtatttctca aattcatccg tgaggttctc
     7441 ggcggcgtag atgacgttgt tcaggtgcgc cgtcacctcc gccatcgtcc acttttcctt
     7501 catccagtcc aaggtgatgg cgctcaacat gccctgggcg tccgtgctca accaattgat
     7561 ggcgacgttc tgggggatct gcagctgcat acggacgtat ttttcagcga cgggagattc
     7621 gcgcagttgg acgagcttgt cgatgaaatc cttgaaattg gcacccagcc gggcccggtc
     7681 ctcgtcggtc aacgccgcgc gcggatcgcg gtagcccctg tcccgcatga actggtccgc
     7741 ccaaaaaagc gtggtggcga aaaccatatt gcttctctcg ttctgcagtt cgatctcgat
     7801 gatgttccgc aacttgcgga tggtctggtc cagttcggcc ttgtccgccg gagacacgtc
     7861 ggcccgagcc tgtttggcct ggagttcatg gatccacttg ttcaggaagc ggaggcgatc
     7921 ggaatcgggc agttgggcta gttccgacat gccggcgtct ttgagataac gttccacctc
     7981 cggcaggaac gtgcgctgca ccatccccat atattccacc atctggcggg gcgtcatgtt
     8041 gcgggccacg gcaaacgcca tgagcgaccg gtagaggctt tcctgtttca ggtcgcccag
     8101 ttcgctgatt tcctgcggac gaccgtcgac catcacgccg ctctgcaggc cggcgttgat
     8161 gtccgccagc gaggagaggc cggggaagaa ccgctccacc acctcgcggg cggccttgcg
     8221 atacgccgcc ttttcgctgg aggtcaaatg gcggaacatg aaacggttcc atgtggcggt
     8281 gcggctgcgc aggagccgga ggtaggcctc ctggtcggcc cggtccaccg gttcgccccg
     8341 gtgttcccgg gcggccacgg cgaagaggcg caggtcggaa tccatccggg acttcaacca
     8401 ggcgtccttc tcttgctgcg tcagcggctg gcccttggcc tcggcccgat ccatcctgtc
     8461 ctgaagttcc agggcataga acaccgccag cgagaccttc ttcatcaggt tccggtcggc
     8521 ccggtagccg atctccggat cgaacatcaa ttcccgatga tacgggaaca accgcccggt
     8581 gaccggctcg atataggtgg acagaacgtt gggcagatcg tcgggctcgt tgagattttt
     8641 caattccgca tacacgtgcg ggaggatttc acgcagacgg tccatggttt tgtcgtcggc
     8701 ggtgaaacgg taggtcttga acagaccgtc caggagcgtc gcccagtcgt ccacgttata
     8761 caggtgctga tcggtggccg ggatatcgga ttcccgaagg accc
//
LOCUS       DAOTFD010000094         8608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5803_length_8608_cov_4.619432, whole genome shotgun sequence.
ACCESSION   DAOTFD010000094 DAOTFD010000000
VERSION     DAOTFD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5803_length_8608_cov_4.619432"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="P5079_06565"
     CDS             <1..1252
                     /locus_tag="P5079_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY29686.1"
                     /translation="GPRFWRGAGRWAARLFLVSAAAQFALSPLLIHYFHRLSFVGLVS
                     NMFAVPWAAVCLSLGVALFGVDVLFGNGFPTTFLAAVTEKAVLGLWRGVAFFAGWPGA
                     EMVISWNNGQVFALAAAVSVTLVVLAARRRGGKWVLAVWAVALPFFWAGGRPSDRALT
                     VTWLDVGLGDAVVVVSPSRRVTVIDGGSSASAAHRLVPYLRSRGIARIDRLILTHADP
                     PHAGGLRAVLKEFPVGEFLCGAATWRSSVWAEGRELIEDRGVPHRFVEANDAWEEDGV
                     RWRLLAPVPSDPPDPDSQGLVVHLEFGETSALLTADVPVSLQRRLAGVPPSRLTVMQW
                     PHHGRAFPDFDFLAAKNPRWFVVSGDRPKGFVESFSLGGSVRSTALHGSLEWRSDGIE
                     SRLRSLRPPPTDVFRAVVPRVPPL"
     gene            1279..1629
                     /gene="acpS"
                     /locus_tag="P5079_06570"
     CDS             1279..1629
                     /gene="acpS"
                     /locus_tag="P5079_06570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRY29687.1"
                     /translation="MASFSLGVDIVEVSRISRLVRQPRFLRRIYSRRELDYCRGKKNA
                     AQHYAVRFAAKEAVYKALGRARLAHKDISVRNAPSGKPTVELHGPFKSLENKVTISLS
                     HTAAYAVAVALYRA"
     gene            1640..2542
                     /locus_tag="P5079_06575"
     CDS             1640..2542
                     /locus_tag="P5079_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRY29688.1"
                     /translation="MFFLRPKRLRPWLSPRRPTAHKGDFGHVLVLAGSRGMTGAARLC
                     AAGALRAGAGLVTLGVPKSQLPAAARVAPWEAMTLPLPENADGTFRETAFREVEKFVR
                     RRKVTAVALGPGLRVRPGTARLVRKCVSGLEVPVVLDADGLNVLSAFGWPATRRAPLI
                     LTPHPGELARLTGRPVAPDVSSRLTAAGRLARQKEAVCVLKGHRTVVSDGRRGGVNTT
                     GNPGMATGGTGDVLCGLIAALIAQTRGKDLKERMWRAALTGVYVHGLAGDLAARRKTR
                     ISLTAGDVLESLPDAFRKTFGPKI"
     gene            2542..3747
                     /locus_tag="P5079_06580"
     CDS             2542..3747
                     /locus_tag="P5079_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRY29689.1"
                     /translation="MKTPLPRMDQDDRRWWKTAVVLRRKIHRYPELGRREFRTTALIA
                     GTLKAARIPFRRIRPTGIVAWLEGRRGGPCVALRADMDALPLDEAVRWPGRSRRPGVM
                     HACGHDAHTAMLLATALKLARRRDRLAGTVKFFFQPDEEASGGATLLVRQGALSSPQV
                     DAVFGLHVNPRLPSGTVGVKAGPLMASVDRFTLTIRGEGGHGAYPHEGRDAVVIASHA
                     VQALQTLVSRQVDPVEPVVVSVGVIEGGRRFNILAEEVKLVGTVRTLSDEWRRKIPAY
                     MKKTVFGVVRSLGGRCRLEYEALGDSVVNDPSMAEVARSAAVKALGLRRVISLDRPSM
                     GGEDFSEYMRRTPGCYVYLGSGADARSRKPWHHPAFFLHDESMAWGIRYLSVLAEESL
                     RRLTANFRR"
     gene            3753..3962
                     /locus_tag="P5079_06585"
     CDS             3753..3962
                     /locus_tag="P5079_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29690.1"
                     /translation="MLSTLIGFIVACVSLWGMWRWRTDLWMVLRGFLPLCFFLGGVIA
                     MVIGISRLTGDDKHDKLKHLEKTPS"
     gene            3991..4992
                     /gene="thiL"
                     /locus_tag="P5079_06590"
     CDS             3991..4992
                     /gene="thiL"
                     /locus_tag="P5079_06590"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRY29691.1"
                     /translation="MAGLGQVRRLGEWGLLRRFLPDLARRVSRRVTVGPGDDAAVVRL
                     DGGLWAVTTDMLVEGVHFRTAWTDGRDLGHKALAVNLSDLAAMGDVEPGFGVVSLGIS
                     PRTPVDYVDNFYRGLRVLARRHRFDIVGGDTVRADRIVVSIAAVGRFRGGAVPVRRDG
                     ARRGDALLVTGTLGDAAAGLEILSGRGASVPAGVNRFLTRRLLRPQPRLELVRRLLAQ
                     GRLTGLIDSSDGFWRSVRILCGMSGVGAVVHAERLPLSTALRRWAAVRRKDPLDLALV
                     GGEDYELIFTAGPAAARRMERAGLARIVGSVVSRRQGLTVLENLKSRRIPRGFEHFG"
     gene            4994..5488
                     /gene="tsaE"
                     /locus_tag="P5079_06595"
     CDS             4994..5488
                     /gene="tsaE"
                     /locus_tag="P5079_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY29692.1"
                     /translation="MKKTPAFRCETHSEKESLAVAKTLGHLLRPGDWIALIGELGTGK
                     TVFAQGLAEALGCREPARSPTFVLIQTYRPKSGGRATMHHVDLFRLPPGEIPCLAWED
                     LLSDDAVTVVEWAEKAMALWPPFCLPVHIDHLGEDRRAFRFYSVGDRSAEIVRALKSK
                     KAVS"
     gene            5488..6273
                     /gene="tsaB"
                     /locus_tag="P5079_06600"
     CDS             5488..6273
                     /gene="tsaB"
                     /locus_tag="P5079_06600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRY29693.1"
                     /translation="MKLLALETIGTQMGACLWEDLRPRGFKTEKGGDNLMPLLDTLFR
                     KAAWSPADLDAVAVDVGPGRFTGIRIGVTAARTLGQVLQKPVVEVNSLEAMAAQGAGW
                     EAGKSFVWKFPHDLLCVLSEALHDEIYMAVWRFHRLADRGRTGAPERASHTFGHHFKL
                     VHAPSLFPFGRFLSFFQSCFQQQTLLFVGPGAHRYRKSLQGAFKSRARFGPALENVDV
                     RWVAALGSEKFFRREVKSFGQVQPLYLRPSYVEERLGQNTAQN"
     gene            6298..6756
                     /gene="rimI"
                     /locus_tag="P5079_06605"
     CDS             6298..6756
                     /gene="rimI"
                     /locus_tag="P5079_06605"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRY29694.1"
                     /translation="MAISDRRLTCVFDGLAASDVPALAEIEKLSSPSPWTSAQIEVEL
                     KKPVSCFLTAREEGRPVGFGGFWLVEGEAQLATLAVHPSRRRRGIGGELLRRLCGAAR
                     DRGARFMSLEVRVGNAAALRLYGAQGFVETSRRPAFYEGKETAVLMEKKL"
     gene            6753..>8608
                     /locus_tag="P5079_06610"
     CDS             6753..>8608
                     /locus_tag="P5079_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29695.1"
                     /translation="MKRRSPQRAFLLFVLGCAAALCFSAPAAASTPGREDAYHLYLRA
                     LLNERFGKLEEALAEYEKVATLDPSASFVHEAMSAVALRLSQMDKALAEAEKVAQLEP
                     VEAHSYLLIGRVRLARGELVAARASFDKALAIAPDNAEALLYSAHIRMTGQPAEAIAL
                     YNRFLENNPDSLEARVRIAELQQRLGDLDAAAATWKRILDANPSDFSAHLALAHIYEI
                     RQSTSDALAEYEVCRALNPQDAGILLRLGELYFRFGDSAHALDAFREAFGRAPDDPTV
                     NFWLALLAEERKDWKEASQRMAVVAKSNPDSSVLFRQAYYDSQAGDGRRAIRSLEKLH
                     KKDPENPEFLYYLALAYEDIHEPRSAVRWMEKTLAVAPDNADAHFHAAVNWDLLKKFD
                     RAEPHLRRALEINPAHAAALNYLGYSWADRGLHLPQALEYIQEAVRLDPENGAFLDSL
                     GWVYFKMDRFAEAEGVLARATARIDDAVVWEHYGDTLEKLGKTAEAVRAWQEGLFLEP
                     KNAALRKRLKGKNTGRVLPATAARRLLKRVEGNFRQLASLGGFLRIGGRDQNRPFQFQ
                     GLFTYARPSQFRFEILGPFYVPQFLLVRNADGFRWIPRDSPYAVGQSQEDWLR"
BASE COUNT         1250 a         2744 c         2976 g         1638 t
ORIGIN      
        1 cggcccgcgt ttttggagag gcgcggggag gtgggcggcg cggctgttcc tggtgagcgc
       61 ggcggcccag ttcgcgcttt ccccgctcct gatccactat ttccaccggc tgtcgttcgt
      121 gggcttggtg agcaacatgt tcgccgtccc ctgggcggcg gtgtgcctgt ctctcggcgt
      181 ggcgctgttc ggcgtggacg tgttgttcgg aaacggtttt ccgacgactt ttctggccgc
      241 cgtcacggaa aaggcggtcc tgggattgtg gcgcggggtg gcttttttcg ccggttggcc
      301 cggggcggag atggtcattt cctggaacaa cgggcaggtg ttcgccctgg ccgcggcggt
      361 ctccgtgacg ctggtggtcc tcgccgcgcg ccgtcgcggg ggaaaatggg ttttggccgt
      421 ctgggccgtc gccttgccgt tcttctgggc ggggggccgc ccgtcggaca gggcgctcac
      481 cgtcacctgg ctcgacgtcg ggttggggga cgcggtcgtg gtggtctctc cgtcgcgccg
      541 tgtcacggtg atcgacggcg gttcctcggc ctccgcggcg caccgcctgg tcccctatct
      601 gcgctcccgc ggcatcgcgc ggatcgaccg gctcatcctg acccacgccg atccgccgca
      661 cgccggcggg ctgcgggcgg tcctgaagga atttccggtg ggcgagttcc tctgcggcgc
      721 ggccacgtgg cggagttccg tgtgggcgga aggacgggaa ctcatcgaag acagaggagt
      781 gccgcaccgg ttcgtcgagg ccaacgacgc gtgggaggag gacggcgtgc gttggcgcct
      841 gctggctccg gtcccttcgg atccgcccga ccccgactcc cagggattgg tggtccatct
      901 ggaatttggg gagacctcgg ccctgctgac ggcggatgtg ccggtgtctc tgcagcgccg
      961 cctggccggc gtcccgccct cccggttgac ggtgatgcaa tggcctcacc acggccgggc
     1021 gtttcccgat ttcgatttct tggccgcgaa aaatcccaga tggtttgtcg tttccggaga
     1081 ccggccgaaa gggtttgtgg aatcattttc attgggcggg tcggtgagga gcaccgccct
     1141 tcacgggtcc ctggaatggc ggagcgacgg catcgaatcc cgcttgcgct ccctccgtcc
     1201 gccgcccacg gatgtttttc gcgccgtggt cccccgcgtt ccgcccctgt aacgcaaaaa
     1261 atttgataaa ataaccgaat ggcgtctttt tcgctcggtg ttgacatcgt cgaggtctcc
     1321 cgcatctccc gtctcgtccg ccagccccgc ttccttcgga ggatctattc caggagggaa
     1381 ttggattact gccggggcaa gaaaaacgcc gcacagcatt acgcggtccg tttcgccgcc
     1441 aaagaggcgg tgtacaaggc gttggggcgg gcgcggctgg cccataagga catctcggtg
     1501 cgcaacgcgc ccagcggcaa gccgacggtc gaactccacg gtccgttcaa atcactggaa
     1561 aacaaggtca ccatctccct ctcccacacc gcggcctacg cggtggcggt ggctctttac
     1621 cgggcctgag agtcggattt tgttctttct ccgtccgaaa cggcttcgtc cctggctgtc
     1681 tccccgccgc ccgacggcgc acaaggggga tttcggccac gtgttggtgc tggccgggtc
     1741 gcgcgggatg acgggcgccg cccggttgtg cgcggccggg gcgctgcgcg ccggcgccgg
     1801 tcttgtcacc ctgggcgtgc cgaaaagcca gcttcccgcg gcggcgcgcg tggctccctg
     1861 ggaagcgatg acgctcccgt tgccggaaaa cgccgacggc acctttcgcg agaccgcttt
     1921 tcgggaggtc gagaagttcg ttcgccggcg gaaggtcacg gccgtggcgt tggggccggg
     1981 cctccgggtg cgtcccggaa cggcgcggct ggtgcggaaa tgtgtgagcg ggctggaggt
     2041 gcccgtcgtg ctggacgccg acggactcaa cgttttgagc gctttcggtt ggccggcgac
     2101 gcggcgggcc ccgttgattt tgacgccgca tcccggcgag ctggcccggt tgaccggtcg
     2161 tcccgtggcg ccggacgttt cgtcccgcct gacggccgcc ggccggctgg ctcggcagaa
     2221 ggaagccgtg tgcgtcctga aaggccatcg caccgtcgtg tccgacggtc gccgcggcgg
     2281 cgtgaacacc acgggaaatc ccggtatggc caccggcgga acgggcgacg tgttgtgcgg
     2341 cttgatcgcc gcgctcatcg cccagacgcg gggcaaagat ttgaaagaac ggatgtggcg
     2401 ggccgccttg acgggggtgt acgtgcacgg tttggccggc gacctggccg cccggcgcaa
     2461 gacccggatc tccttgacgg ccggcgacgt tctggaaagt ctccccgacg ctttccggaa
     2521 gacgttcgga ccgaagattt gatgaagacc ccgctgcccc gcatggacca agacgaccgc
     2581 cgctggtgga agaccgccgt tgtcctccgc cggaagatcc accgttatcc cgaactgggc
     2641 cggcgggagt tccgcaccac ggcgctgatc gccggaacat tgaaggcggc gcgcatccct
     2701 tttcgccgca tccgccccac ggggatcgtg gcgtggctgg aggggcgccg gggcgggccg
     2761 tgtgtggcgc tgcgcgccga catggacgct ttgccgttgg acgaggcggt gcgctggccg
     2821 ggccggtcgc gccggccggg cgtgatgcac gcctgcggcc acgacgcgca caccgccatg
     2881 ctgctggcga ccgctctgaa actggcgcgt cggcgggacc ggctggccgg caccgtcaaa
     2941 tttttttttc aaccggacga ggaggcttcc ggcggggcga ccctcctggt ccgccagggc
     3001 gccttgtcct ccccccaggt ggacgcggtg ttcgggcttc acgtcaaccc ccgcctgcct
     3061 tccggcaccg tcggcgtcaa agcggggccg ctcatggcgt cggtggaccg gttcaccctg
     3121 acgatccgcg gcgagggggg gcacggcgcc tacccccacg agggccgcga cgccgtcgtg
     3181 atcgcttcgc acgccgttca ggcgctccag accctggtgt cccggcaggt ggacccggtg
     3241 gagccggtgg tggtgagcgt cggcgtcatc gagggcggcc ggcggttcaa tattttggcg
     3301 gaggaagtca aactggtcgg caccgtgcgc acgttgtcgg acgaatggcg ccggaagatc
     3361 cccgcgtata tgaagaaaac ggttttcggg gtggtccggt ccctgggcgg ccgatgccgg
     3421 ctggagtatg aggcgctcgg ggattccgtc gtgaacgatc cgtcgatggc cgaggtggcg
     3481 cggagcgcgg cggtgaaggc gctcgggctc cggcgggtga tctcgttgga ccgcccgtcc
     3541 atgggcggtg aggatttttc cgaatacatg cgtcggaccc cgggctgtta cgtctatctg
     3601 ggcagcggcg ccgacgcccg ttcccggaaa ccttggcatc atcccgcttt tttcctccac
     3661 gacgaatcca tggcgtgggg gatccgttac ttgtcggttt tggcggaaga gtcgttgcgt
     3721 cggctcaccg cgaattttag gaggtagcca tcatgttaag cactctgatc ggattcattg
     3781 tggcgtgcgt cagcctgtgg gggatgtggc ggtggcggac cgatctgtgg atggtcctgc
     3841 gcggtttcct gccgctctgc ttctttctgg gaggcgtgat cgccatggtc atcgggatct
     3901 cccgtctgac gggcgacgac aagcacgaca aactcaaaca cttggagaag actccttcct
     3961 aacccggccc ggtcggatcg aggatcgcgc atggccggac tcggccaggt ccgtcggttg
     4021 ggggagtggg gacttctccg gcgttttctg ccggacctcg cccgtcgtgt ttctcggcgg
     4081 gtgacggtgg ggccgggcga cgacgccgcg gtggtccggc tcgacggcgg attgtgggcc
     4141 gtcaccacgg acatgctggt ggaaggggtc catttccgca ccgcctggac cgacgggcgg
     4201 gacctgggtc acaaggcgtt ggcggtcaat ttgtcggatt tggcggccat gggggacgtg
     4261 gaacccggtt tcggggtggt gtccctgggg ataagccccc ggacccctgt ggattatgtg
     4321 gataatttct accggggact ccgggttttg gcccgccggc accgcttcga tatcgtcggc
     4381 ggcgacacgg tgcgggccga ccggatcgtg gtctccatcg cggcggtcgg gcggttccgg
     4441 ggcggcgccg ttccggtgcg gcgtgacggt gcgcggcggg gggacgcgct tctggtcacg
     4501 gggacgttgg gcgacgccgc cgcagggctc gagatcctgt cgggccgggg ggcgtcggtc
     4561 ccggccgggg tcaatagatt tttgacacgc cggttgttgc ggccccaacc gcggctggaa
     4621 ctggtccgcc gtctgctggc ccagggccgg ctcaccgggc tgatcgattc gtcggacggt
     4681 ttctggcggt cggtgcggat tttgtgcggg atgtcggggg tgggggcggt ggtccatgcg
     4741 gagcggctgc ctctgtcgac ggcgctccgg cgctgggcgg ccgttcgccg gaaagacccc
     4801 ctcgacttgg ctttggtggg cggcgaggat tacgagctca ttttcacggc ggggccggcc
     4861 gcggcgcggc ggatggaacg ggccggcctg gcccgcatcg ttggttccgt cgtctcgcgc
     4921 cggcaaggac tgacggtgtt ggaaaatctc aaatcacgga ggatccctcg tgggttcgaa
     4981 catttcggtt gacatgaaga agacgcccgc ttttcgctgc gagacccatt cggaaaaaga
     5041 gtccctggcc gtggccaaga ccctcggcca cctcctccgt cccggggatt ggatcgccct
     5101 tatcggcgaa ctgggcaccg gaaaaacggt tttcgctcag ggactggcgg aggcgctggg
     5161 ctgccgcgag ccggcccgca gccccacctt cgtcctgatc cagacctaca ggcccaagtc
     5221 gggcggccgg gcgaccatgc atcacgtgga cctcttccgg cttccgccgg gcgagatccc
     5281 ctgcttggcc tgggaggacc tcctctccga cgacgccgtc accgtggtgg agtgggcgga
     5341 gaaggccatg gccctgtggc cgccgttctg tctgcccgtg cacatcgacc acttgggcga
     5401 agaccgccgg gcgttccgtt tctattcggt gggggaccgg tcggcggaga tcgttcgggc
     5461 gttgaaatcc aaaaaggcgg tgtcctgatg aaacttctgg cgttggaaac catcggtacc
     5521 caaatgggcg cgtgtctgtg ggaagacctg cgtccgcgcg gttttaagac ggaaaaaggc
     5581 ggggacaacc tcatgccttt gttggacacc ttgttccgaa aagccgcctg gtcgcccgcc
     5641 gacctggacg cggtggcggt ggacgtgggc ccgggccggt tcacgggcat acgcatcggc
     5701 gtcaccgcgg cccgcaccct cggccaggtc cttcagaaac ccgtggtgga ggtgaacagt
     5761 ttggaggcca tggccgccca gggggcggga tgggaggccg ggaaatcctt cgtgtggaag
     5821 tttccgcacg accttctctg cgtgttgtcg gaggccctgc acgacgagat ctacatggcg
     5881 gtgtggcggt tccaccggct ggcggaccgc ggccgcaccg gcgcgccgga acgggcctcc
     5941 catacgttcg gacaccattt caaactggtg cacgctccct cgctctttcc gttcggtcgg
     6001 ttcttgtcct tcttccagag ttgttttcag cagcagaccc tcctgttcgt ggggccgggg
     6061 gcgcaccgct accgaaaaag tctgcagggc gcgttcaagt cccgggcgcg gttcggaccc
     6121 gccctggaga acgtggacgt ccgttgggtg gcggcgctgg gttcggaaaa gttcttcagg
     6181 cgcgaggtga agtctttcgg gcaggtccag ccgctctatc tccgtccttc ctacgtggaa
     6241 gagcgcctcg gtcaaaatac cgcccaaaac tgagccgtcg tcggtttcac gcgtcccatg
     6301 gcgatttccg atcggcggct cacctgcgtt ttcgacgggt tggcggcctc cgatgtcccg
     6361 gctctggcgg agatcgaaaa actttcctcg ccttccccct ggacgtccgc gcagatcgag
     6421 gtggaactca aaaaaccggt gtcctgtttt ctgaccgcgc gagaagaagg ccgtccggtc
     6481 ggcttcggcg ggttttggct ggtggagggg gaagcccagc tggccaccct ggcggtccat
     6541 ccgtcccggc gacggcgggg catcggcggc gaactccttc ggcggctgtg cggggccgcg
     6601 cgggaccgcg gcgcccgttt catgagtttg gaagtccgcg tcggcaacgc ggccgccctc
     6661 cgtctatacg gagcccaggg gtttgtcgag acgtcccggc gtcccgcctt ctacgaggga
     6721 aaagaaaccg ccgtgttgat ggagaagaaa ttgtgaaacg ccgcagtccc caaagagcct
     6781 tccttctttt tgtcctcgga tgcgcggcgg cgctctgttt ttcggcgccc gcggcggcct
     6841 ccacgcccgg ccgggaagac gcgtatcacc tgtatctccg cgccctgttg aacgagcgtt
     6901 tcgggaagct ggaggaggcg ctggcggagt atgaaaaagt cgccaccctc gatccgtcgg
     6961 cctcgttcgt gcacgaggcc atgtccgccg tggccctgcg gctcagtcag atggacaagg
     7021 ccctggccga ggcggagaag gtcgcccagt tggagcccgt ggaagcgcat tcctacctgc
     7081 tcatcggccg cgtccggctg gcccgggggg aactggtggc cgcgcgcgcc tcgttcgaca
     7141 aggccttggc catcgctccc gacaacgccg aggcgctcct ctattccgcc cacatccgca
     7201 tgaccggcca gcccgccgag gccatcgcgc tctacaaccg tttccttgag aataatcccg
     7261 attccttgga ggcgcgcgtg cgcatcgccg aactgcaaca gcgtttgggc gatctggacg
     7321 ccgccgcggc cacgtggaag aggattttgg acgccaaccc gtcggatttc tccgcgcacc
     7381 tggccctggc ccatatctat gaaatccggc agtccacgtc cgacgccctt gccgaatacg
     7441 aggtttgccg cgcgctgaac ccgcaggacg cggggattct cctgcggctg ggagaacttt
     7501 attttcgttt cggggattcc gcccacgcgc tcgatgcctt tcgagaggcc ttcggccgcg
     7561 cgccggacga ccccacggtc aatttttggc tggcgttgct tgccgaagaa cgcaaggatt
     7621 ggaaagaggc ctcccagcgc atggccgtgg tggcgaaatc caatccggat tcgtccgttc
     7681 ttttccgcca ggcctattac gacagccagg ccggagacgg ccggcgggcc atccgttctc
     7741 tcgaaaaact gcacaagaag gaccccgaga accccgagtt cctctattac ctggcgctgg
     7801 cctatgaaga catccatgag ccgcgttcgg ccgtgcgctg gatggaaaaa acattggcgg
     7861 tcgccccgga caacgccgac gcccactttc acgccgccgt caattgggat ctgctgaaaa
     7921 aattcgatcg ggccgaaccc catctccgcc gggcgctgga gatcaatccc gcccacgccg
     7981 ccgcgctgaa ttacctcggc tacagctggg ccgaccgcgg ccttcatctg ccccaggcgt
     8041 tggaatacat ccaggaggcg gttcggctcg accccgaaaa cggcgccttc ctcgattcgc
     8101 tgggctgggt ctatttcaag atggaccggt tcgccgaagc cgaaggcgtc ctcgcgcggg
     8161 cgaccgcccg catcgacgac gccgtggtgt gggaacatta cggcgacacg ctggaaaaac
     8221 tcgggaaaac cgccgaggcc gtccgcgcct ggcaggaggg gctcttcctt gaacccaaga
     8281 acgccgccct ccgaaaacgg ctcaagggga aaaacaccgg ccgcgtcctc ccggcgaccg
     8341 cggcccgccg cctcctgaaa cgggtggagg gcaacttccg ccaactcgcc agcctcggcg
     8401 gctttctgcg gatcggcggc cgcgaccaaa accggccctt tcagtttcag gggttgttca
     8461 cctacgcccg tccgtcgcag ttccggttcg agatcctggg gccgttctat gtccctcagt
     8521 tccttctcgt tcggaacgcc gacggcttcc ggtggattcc gcgcgattcg ccttacgcgg
     8581 tgggacagtc ccaggaagac tggctccg
//
LOCUS       DAOTFD010000095         8604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5808_length_8604_cov_4.486958, whole genome shotgun sequence.
ACCESSION   DAOTFD010000095 DAOTFD010000000
VERSION     DAOTFD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_8604_cov_4.486958"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="P5079_06615"
     CDS             <1..1244
                     /locus_tag="P5079_06615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRY29696.1"
                     /translation="VLESKAGAKMRLLWTCDKYGKPVPPRGVQKPRFTRDWRDVVNDP
                     HVDIVVELIGGYEPARTLVLAALKSRKHVATANKALLAKYWGEMSVAAQQSGALLYFE
                     AAVGGGIPVIQGINEGLAANRIEKIYGILNGTTNYILGRMTEDGMDFAAALKGAQEAG
                     FAEADPTFDIEGIDAAHKLAVLASLASGAWIRLEDVTREGISSLDVWDVHFARERLGM
                     VTKLLGIVELGKDRFSARVHPTLIPGTHPFANVPREYNAIFVHGDAAGDVMFYGKGAG
                     QMAASSAVVSDVIYLARQVANGTAGQIPYVTYNPAVQLKPIPFDQLQCRHYLRFTTVD
                     RPGVLAQITRFLARQKVSISSVHQEDTESALGAAVRGGVPIVVLTHKAPEGRVREAAR
                     NIDRLAVVRRKTVHLRIEDSF"
     gene            1252..2316
                     /gene="thrC"
                     /locus_tag="P5079_06620"
     CDS             1252..2316
                     /gene="thrC"
                     /locus_tag="P5079_06620"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRY29697.1"
                     /translation="MSYQGIIEKYRKHLPVSAATPVVSLKEGNTPLIPVPRLARWAGA
                     PGLRLFVKYEGLNPTGSFKDRGMTMAISKALEEKSRAVVCASTGNTSASAAAYASRAG
                     LRCVVLIPAGAIALGKLSQAVMHGAEVYSVAGNFDDALEMVKKVSATGKLTLVNSINP
                     YRIEGQKTGAFEIVDDLSDAPDFQFMPVGNAGNITAYWKGYKEYSDLKKASRRPKMMG
                     YQAEGAAPIVRGEPVKSPQTIATAIRIGNPASWKQAEAARDESGGRIDSVTDDEILSA
                     YKALASQEGVFCEPASAASVAGLKKYAARDDAFARSAATVVCILTGHGLKDPDRAMAV
                     MPAPKSVEPRADAILKELGL"
     gene            2379..2765
                     /locus_tag="P5079_06625"
     CDS             2379..2765
                     /locus_tag="P5079_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29698.1"
                     /translation="MPAVKEKNSLKKFLSSSTPYLLLAGFFYAAGFVSALREAGWDAI
                     SWSRFAVLFPTVLLVVGFLNRRRRSRRLAFFLGVAGAAASVAILWALGAHLDHPADVP
                     WGVFGRAGGLYLGLTFAGYFQFRADR"
     gene            2770..4116
                     /locus_tag="P5079_06630"
     CDS             2770..4116
                     /locus_tag="P5079_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRY29699.1"
                     /translation="MKIMVQRISDIVWGPPMLVLLVGTGLLLTVVLRGLQFRLLPKAL
                     YLAFWVRHEEGASGDVSHFQALMTALAATVGTGNIAGVATAIALGGPGALFWMWVTGL
                     VGMATKYSEALLGVKYRVTESDGTMSGGPMHYIARGLGVQWLGTVFALCAAVAAFGIG
                     NMVQANSVAHAVHDALRVPTYVTGLVLAVATALVLIGGIQSIARTSCWMVPVMCVVYM
                     LGGGVILVMNADKIPQVLALIFRDAFTGTAAAGGFGGASVMLAVRYGVARGVFSNESG
                     LGTAGFAAAAAKSSHPALQALVSMTQTFLDTLVVCSVTGLVILVTGAWTQPDAAGRLL
                     TGSPLTMAAFSTGLPGHWGGWVVTIGLVLFAYSTLIGWSYYGEKAFVYLFGLRVVRPY
                     RIVFCVAVFFGAMTELTMVWNLADIANGLMALPNLIGLLCLLPVVLKESRHSWGES"
     gene            4139..4450
                     /locus_tag="P5079_06635"
     CDS             4139..4450
                     /locus_tag="P5079_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRY29700.1"
                     /translation="MTEQNPAVPPQELSIEMDDATANGVYSNLALITHSETEFVLDFI
                     FHSPQPPKARVRSRIITNPAHMKRFLLALQDNISRYEARFGPIRTSGQPPADNKVGFF
                     H"
     gene            4468..5760
                     /locus_tag="P5079_06640"
     CDS             4468..5760
                     /locus_tag="P5079_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRY29701.1"
                     /translation="MNGPPRVELLCVGSELLSGKVNTHAAAVAGRLAEVGLALSREVT
                     VGDDPAEMEKVFRESWRRAQVVLCTGGLGPTFDDLTRDVWAKVTHRPLRFQKPLMDDI
                     RNKFRRRRWPMPKENRRQTYVLAGAAVMQNPYGTAPGQVLVAGGKVLALFPGPAREFF
                     PMMEGKFLPFLRTRLPPRTRKTKIYRLFNFSESAVDERLDGLRRRSRAPGKIEVTWGI
                     LAQRFIIDVKVTVAGPAPAAVESALARIHSAVLAEFGGDVYGFGEETLQECVGRDLRR
                     RKETVSTAESCTGGLVAEKLTRVPGSSDYFMQGSVTYSNRSKRRLLGVRQETLRRHGA
                     VSAECAREMAAGCRRRAGTDWALALTGVAGPSGGTPQKPVGLVYIALAGKRGVSVTEH
                     RFSGTREDIRERSALTALDRLRRALQKKRSGGRSGTSG"
     gene            5764..6540
                     /gene="rsmA"
                     /locus_tag="P5079_06645"
     CDS             5764..6540
                     /gene="rsmA"
                     /locus_tag="P5079_06645"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY29702.1"
                     /translation="MRQKWGQNFLVDKNTAERIVESLAPFSGPVIEIGPGKGILTGLL
                     AERTSDLTAVELDRDLAAGLAQGWPKVRVVAEDFLEWPFPAGPGPVAFIGNLPYSAAN
                     AILRKVLDWTGWDRAVFMVQKEVADRIVAAPCSRDYGILTLAVQGKAEAESLFDVPPR
                     CFQPRPQVTSTVVRLKRWPASKIRDEKKFFRAVRAAFGQRRKTIGNSLSRVLGLEKNA
                     VEDCLKRAGFDVVRRAETFTLEEFNRLAEILPENGQGQRQ"
     gene            complement(6558..7781)
                     /locus_tag="P5079_06650"
     CDS             complement(6558..7781)
                     /locus_tag="P5079_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY29703.1"
                     /translation="MEIKAAQILKNESVRVYFDPILSVKKKSLIGLSEVGCGVDPETG
                     ADIPANALLRLDASPGTRLQMDRLLRKKALETFKGLPGKSSDLLLFVRLDTAIIDSNV
                     VGSGHLSGLVRDLGLDPNNIVIELAASGVPDTSALKEFIQTYRRHGFLIALNEVGSGH
                     SNMNRLMLARPDLLKIGSGLTKNIDRDYYCREIFTALTHLSKKLGALIVATGMEREEE
                     AVSALGLGADMIELGLRLSPDGDPAETRSLLEGTMDRIGAGLKDTLVRKNSDRREQAR
                     GHEKLVTEILRGLNKSSREHFDAQLAEVLEKHPSLECAYILDRNGVQVTETVFMKGKT
                     YKKKSLFAPAKRGADHSAKEYFYLLNDAFVNRFTTEPYISLASGSLCLTVSSLFRDAA
                     NQEFVFCADIPLESA"
     gene            complement(7895..>8604)
                     /locus_tag="P5079_06655"
     CDS             complement(7895..>8604)
                     /locus_tag="P5079_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY29704.1"
                     /translation="VIEAKDDYNISRGFLFTYAIPYTHTGVKWDKAWGDAFTTMLGVV
                     NGWDNLQDNNKGKTLHAGVGLLPKEKISLTVSGTYGPEQTTPVPPTPSTEKNSRSVVD
                     TILKVSPTDKLTLIANHDWGIEEGLVSAQADNTTYNWQGLALYAVYGFTDDTSAALRW
                     ETFDDEGELSNQSRLGLNQPVVLHSLTATLQRKHNGVIYRLEYRQDDATRKVFFDNKT
                     LPDDTQSTVGAQIIYAF"
BASE COUNT         1444 a         2626 c         2822 g         1712 t
ORIGIN      
        1 ccgtgttgga gagcaaggcg ggggcgaaga tgcgcctgct gtggacgtgc gacaaatatg
       61 ggaaacccgt cccgccgcgc ggcgtccaga agccccgctt cacgcgcgac tggcgcgacg
      121 tggtgaacga tccccacgtg gacatcgtgg tggaactgat cggcgggtac gagccggcgc
      181 gcaccctggt gctggccgcg ttgaaatcga ggaaacacgt ggccaccgcc aacaaggcgc
      241 tcctggcgaa atattggggg gagatgagcg tcgcggccca gcaatcggga gcgctcctct
      301 atttcgaggc cgccgtcggc ggagggatcc cggtcattca aggcatcaac gaggggctgg
      361 cggccaaccg catcgaaaag atctacggta tcctcaacgg caccaccaac tacatcctcg
      421 gccgcatgac ggaggacggc atggatttcg ccgccgccct gaaaggcgcc caggaggccg
      481 ggttcgccga ggccgacccc accttcgaca tcgaagggat cgacgccgcc cataagctgg
      541 cggtcctcgc ctccctggcc agcggcgcct ggatccgcct ggaggacgtg acgcgcgagg
      601 ggatctcctc cctcgacgtg tgggacgtcc acttcgcgcg cgaacggctc gggatggtga
      661 ccaaactgtt gggcatcgtc gagcttggga aagaccggtt ttccgcgcgc gtccacccca
      721 cgctcattcc gggcacccat cctttcgcca atgtgccgag ggaatacaac gcgatcttcg
      781 tgcacggcga cgccgccggc gacgtcatgt tctacggcaa gggggccggc cagatggcgg
      841 cttccagcgc cgtcgtttcc gacgtcatct acctggcacg ccaggtggcc aacggcaccg
      901 cgggacagat cccgtacgtg acctacaatc ccgccgttca attgaagccg atccccttcg
      961 accagctcca gtgccgtcat tacctgcggt tcaccacggt ggaccggccg ggcgtcctgg
     1021 cgcagatcac ccggttcctg gcccggcaga aagtttccat ctcctccgtg caccaggaag
     1081 acacggagtc ggcgttgggg gcggccgtcc gcggcggcgt gcccatcgtg gtcctcacac
     1141 acaaagctcc cgaggggagg gtgcgcgagg ccgcgcggaa catcgaccgt ctcgccgtcg
     1201 tccggcggaa gaccgtccac ctccgcatcg aggactcgtt ttaaccgccc catgagctat
     1261 cagggaatca tcgaaaaata ccggaagcac cttcccgtct ccgccgccac gccggtggtg
     1321 tcgctgaaag aaggcaacac cccgctcatt cccgttcccc ggttggcccg atgggccggc
     1381 gcgcccggtc tccggctctt tgtgaaatac gaggggttga accccaccgg gtccttcaag
     1441 gaccgcggca tgaccatggc catttcaaaa gcgttggagg aaaaatccag ggccgtggtg
     1501 tgcgcttcca ccggcaacac ttccgcctcg gcggccgctt acgcgtcgcg ggcggggctc
     1561 cggtgcgtgg tgctgatccc ggccggcgcc atcgccctcg ggaaactctc ccaggcggtg
     1621 atgcacggcg ccgaggttta ttccgtggcg gggaatttcg acgacgccct ggagatggtg
     1681 aagaaagtga gcgccaccgg gaaactgacg ctggtcaact ccatcaaccc ctatcgcatc
     1741 gaggggcaaa agaccggggc gttcgagatc gtggacgatt tgtccgatgc gccggatttc
     1801 caattcatgc ccgtcgggaa tgccggcaac atcaccgcct attggaaagg ttacaaggaa
     1861 tattccgacc tgaagaaggc atcccggcgg ccgaagatga tgggatatca ggccgaaggg
     1921 gcggccccca ttgttcgagg ggagcctgtg aagtcccctc agaccatcgc caccgccatc
     1981 cgcatcggca accccgcgtc ctggaaacag gccgaggccg cccgcgacga gtccggcggg
     2041 cgcatcgatt cggtcaccga cgacgaaatc ctctcggcgt ataaggcgtt ggcgtctcag
     2101 gaaggggtct tctgcgaacc cgccagcgcg gcttccgtcg ccggcttgaa aaaatacgcc
     2161 gcccgggacg acgcgttcgc ccggtccgcg gccacggtgg tgtgcatcct caccgggcat
     2221 gggttgaagg acccggaccg cgccatggcc gtgatgccgg ccccgaaatc cgtcgaaccg
     2281 cgcgccgacg ccatcctgaa agaactcggg ctgtgatttt ttccgcccgt taagcgcccc
     2341 gttcggtttc agcagagatc ctgaccagaa gcccctgaat gcccgccgtt aaagaaaaaa
     2401 attccctcaa aaagtttctt tcctcatcga ccccctatct tctcctggcg ggttttttct
     2461 atgcggccgg ttttgtgtcg gcgctccggg aggccgggtg ggacgccatt tcgtggagcc
     2521 gcttcgccgt tttgttcccc accgttcttt tggtcgtcgg tttcctcaat cgccgccgcc
     2581 gatcgaggcg tctcgctttt tttctcggcg tggccggggc cgcggcttcc gtcgcgatcc
     2641 tgtgggcttt gggggcgcac ctggaccatc cggcggacgt tccctggggc gtcttcggcc
     2701 gtgcgggggg gctctatctg gggctgacct tcgccggcta cttccaattt cgggccgacc
     2761 ggtagagaca tgaaaatcat ggtgcagagg ataagcgata tcgtctgggg gccgcccatg
     2821 ctggttctgc tggtgggcac gggtctcctt ctgacggtgg ttttgcgggg gctccagttc
     2881 cgcctgcttc ccaaggccct gtatttggcg ttttgggtga ggcacgagga aggggcctcg
     2941 ggcgacgtga gtcatttcca agcgctgatg accgctctgg cggccaccgt cggaaccgga
     3001 aacatcgccg gcgtggccac cgccatcgcc ttgggagggc ccggagcgct tttttggatg
     3061 tgggtcacgg gactggtggg catggccacc aaatattccg aggccctgtt gggcgtcaaa
     3121 taccgcgtga cggaatccga cggcaccatg agcgggggac cgatgcacta catcgcccgg
     3181 ggcctcggcg tccaatggct gggaacggtt ttcgcgctgt gcgccgcggt ggcggccttc
     3241 gggatcggca acatggtgca ggccaactcc gtggcccacg ccgtccacga cgcgctccgc
     3301 gtcccgacct atgtgacggg acttgtcttg gcggtggcca ccgcgctggt gctgatcggg
     3361 ggcatccagt ccatcgcccg aacatcgtgc tggatggtgc cggtgatgtg cgtggtctat
     3421 atgctggggg gcggtgtgat actggtaatg aacgcggata aaataccgca ggtgttggcg
     3481 ctgatttttc gggacgcttt caccggaacg gcggccgccg gcggtttcgg cggggcctcc
     3541 gtcatgctgg ccgtgcggta cggggtggcc cgcggcgttt tctccaacga gtcgggcctc
     3601 ggcaccgcgg gtttcgcggc cgccgccgcc aagtccagcc acccggccct tcaggccctc
     3661 gtctccatga cacagacgtt tttggatacc ctcgtcgtct gttccgtcac cggtctcgtc
     3721 atcctggtca ccggcgcctg gacgcagccg gatgccgccg gccggcttct caccggttcg
     3781 cccctcacca tggccgcttt ttccaccggc cttcccggcc actggggcgg ctgggtggtg
     3841 accatcggac tggtcctttt cgcctattcc acgctcatcg gctggtccta ttacggcgaa
     3901 aaggcgttcg tctatctttt cggactccgc gtcgtccggc cttaccggat cgttttctgc
     3961 gtcgcggtct ttttcggggc gatgacggaa ctgacgatgg tttggaattt ggccgacatc
     4021 gccaacggcc tcatggcctt gccgaacctg atcggtttgc tgtgtctgct gcccgtggtg
     4081 ttgaaagagt cgcggcactc ttggggcgaa tcttaaaaac agaaaagggg aggacgatat
     4141 gactgaacaa aacccggcgg tcccgccgca ggagctttcc attgagatgg acgacgctac
     4201 ggccaacggc gtctattcga atctggcgct catcacgcat tctgagacgg agttcgtgct
     4261 cgacttcatt ttccattcgc cgcagccccc caaggcccgg gtgcgctccc gcatcatcac
     4321 caaccccgcc cacatgaaac ggttcctgtt ggctttgcag gacaacatct cccgctatga
     4381 ggcccgcttc ggcccgatcc ggacctccgg ccagccgccg gccgacaata aggtcggttt
     4441 tttccactga ggcacgtccc cctcgttatg aacgggccgc ctcgggttga gttgctttgc
     4501 gtgggcagcg agctgctgtc cggcaaggtc aacacacacg ccgccgccgt ggccggccgt
     4561 ctggcggagg tggggctggc cctttcgcgc gaggtcaccg tcggggatga tcccgccgaa
     4621 atggagaagg tctttcggga gtcctggcgg cgggcccagg tggtcctttg cacgggagga
     4681 ctcgggccga ccttcgacga cctcacgcgc gacgtttggg ccaaggtgac ccaccgcccg
     4741 ttgcgcttcc aaaaaccgct catggacgac atccgaaata aattccgccg ccggcggtgg
     4801 cccatgccga aggaaaaccg gcgacagacc tatgtgttgg ccggcgccgc ggtgatgcag
     4861 aacccttacg gcaccgcccc gggccaggtg ctggtggcgg gggggaaggt cctcgccctt
     4921 tttccgggtc cggcccggga gttttttccc atgatggagg gaaaattttt gccgttcctc
     4981 cggacgcgcc tcccgccgcg gactcgcaag acgaagatat accggctctt caacttctcc
     5041 gaatcggccg tggacgagcg attggacggc cttcgccgac ggtcgcgcgc gcccgggaaa
     5101 atcgaagtca cctggggcat cctcgcccag cggttcatca tcgacgtgaa ggtcacggtg
     5161 gccggtcccg cgccggcggc ggtggaatcc gcgttggcga ggatccattc cgccgtcctg
     5221 gcggagttcg gcggggatgt ttatgggttc ggcgaggaga cgcttcaaga atgcgtcggc
     5281 cgggacctca ggcgacggaa ggagaccgtg tccacggcgg agtcgtgcac gggcgggttg
     5341 gtggccgaaa aattgacccg cgttcccggc agttccgatt atttcatgca ggggtccgtg
     5401 acctattcca accggagcaa aaggcggctc ttgggcgttc ggcaggaaac gctccggcgg
     5461 cacggcgcgg tttcggcgga atgcgcccgg gagatggcgg cggggtgccg tcgccgtgcg
     5521 ggcaccgatt gggccttggc tctcaccggc gtcgccggcc cttccggcgg taccccgcag
     5581 aaacccgtcg gtctggtgta catcgccttg gcggggaaga ggggggtctc cgtcaccgaa
     5641 catcgatttt ccgggacgcg ggaggatatc cgcgaacgga gcgcgctcac ggcgctggac
     5701 cggcttcgcc gggcgttaca gaaaaaacgg tccggtggcc ggtccgggac ttccgggtga
     5761 acgatgcggc aaaagtgggg acagaacttt ctcgtcgata agaacacggc cgaacggatc
     5821 gttgaatcgc tggccccgtt ttcagggccg gtcatcgaga tcggtcccgg caaggggatc
     5881 ctcaccgggc ttttggccga gcggacgtcc gatctgaccg cggtggagtt ggacagggat
     5941 ctggcggcgg ggctggcgca gggatggccg aaggtccgcg tggtggcgga agattttttg
     6001 gagtggccgt tccccgccgg gccgggtccg gtcgctttca tcggaaacct cccctactcg
     6061 gcggccaacg cgatcttacg caaggtcttg gactggaccg ggtgggaccg ggcggtgttc
     6121 atggtgcaga aagaggtggc ggaccggatc gtcgcggcgc cgtgcagtcg ggattacggg
     6181 attttgacct tggcggtgca gggaaaagcg gaggcggagt ctctgttcga tgtgccgccg
     6241 agatgttttc agccgcggcc gcaggtcacc tccaccgtgg ttcgtctaaa gagatggccg
     6301 gcctcgaaaa tccgggatga aaagaaattc ttccgcgccg tccgcgcggc cttcggtcaa
     6361 agacggaaga ccatcggcaa cagcctctcg cgcgtgctgg gactcgaaaa aaacgccgtg
     6421 gaggattgcc tgaaacgcgc cggattcgac gtcgtccgcc gcgccgagac gtttaccctc
     6481 gaggagttca accgcctggc ggaaatcctg ccggaaaacg gccagggaca acgccaatag
     6541 ggggtgtccg gccagggtca cgccgactcc agcgggatgt cggcgcagaa aacgaattcc
     6601 tggtttgccg catcccggaa aagacttgaa acggtgagac acagactgcc ggaagcgagg
     6661 gagatgtagg gctcggtggt gaaccggttg acgaaggcgt cgttgagaag ataaaagtat
     6721 tctttggcgg agtggtcggc cccccgtttc gccggggcga agaggctttt tttcttgtac
     6781 gtttttccct tcataaacac cgtttcggtc acttgaacgc cgtttctgtc caggatgtag
     6841 gcgcattcca gggaagggtg tttttcgagc acctcggcca gttgggcgtc gaaatgttcc
     6901 cgagacgact tgttcagccc tcgtaaaatc tcggtcacga gtttttcatg tccgcgggct
     6961 tgttcccggc ggtcgctgtt tttcctgacg agagtgtcct tgagtccggc cccgatccgg
     7021 tccatggttc cttccaggag ggagcgggtc tccgccgggt cgccgtcggg cgaaagccga
     7081 agacccagct cgatcatgtc cgcgcccagc cccagggcgg acaccgcctc ctcttcccgc
     7141 tccatccccg tcgcaacgat cagcgctccc aattttttcg acaggtgggt caacgcagtg
     7201 aaaatctccc ggcagtaata atcccggtcg atgttctttg tgaggccgct cccgatcttg
     7261 agcaggtcgg gccgcgccag catcagccgg ttcatgttgg aatgcccgga tccgacttcg
     7321 ttgagggcga tgaggaaccc gtgccggcgg taggtttgta tgaattcctt cagggccgag
     7381 gtgtcgggga cgccggaggc cgcgagttcg atcacgatgt tgttcgggtc gaggcccagg
     7441 tcgcgcacga ggcccgacag gtgtcccgag ccgaccacgt tggaatcaat gatggcggtg
     7501 tcgaggcgga cgaaaagaag gaggtcgctg ctcttccccg gaagtccttt gaaggtttcc
     7561 agggcttttt ttcggaggag tcggtccatc tgcaggcggg tgccgggaga cgcatccagg
     7621 cggaggaggg cgttggccgg gatatcggcc cctgtttcgg gatccacccc gcacccgacc
     7681 tcgctgagtc cgatgagaga ctttttttta acggaaagga tggggtcgaa atagacccgg
     7741 acactttcgt ttttcaggat ttgagcggct tttatctcca tgggatcccc tgatccggtt
     7801 tccccggcct cccgaaagaa ttgcgccgtc ggaagaccgg ggagttcttt ttcgtctccc
     7861 ggcttttcgg tcgggagacg gcggagcgtc atgcctaaaa cgcgtagatg atctgtgccc
     7921 ccacggtgct ctgcgtgtcg tcgggcaagg tcttgttgtc gaaaaagacc tttcgggtgg
     7981 cgtcgtcttg gcggtattcc agccgataaa tcaccccgtt gtgtttgcgt tgaagcgtgg
     8041 cggtgaggga atgcagcacc accggctggt tcagccccag ccgagactgg ttggaaagtt
     8101 cgccctcgtc gtcaaacgtt tcccagcgca gggcggccga ggtgtcgtcc gtgaagccgt
     8161 agacggcgta gagggccagg ccctgccaat tgtaggtggt gttgtccgcc tgcgcggaga
     8221 ccagtccctc ttcgatgccc cagtcgtggt tggcgatgag ggtcaacttg tccgtcgggc
     8281 tcactttcag gatggtatcg accacgcttc ggctgttttt ttcggtgctg ggggtgggcg
     8341 ggacgggggt cgtctgttcc gggccgtagg ttccgctcac cgtcagggat attttttctt
     8401 tcggcaggag acccacgccg gcatggaggg tttttccctt gttgttgtct tggaggttgt
     8461 cccagccgtt cacgacgccc agcatggtgg tgaaagcgtc tccccaggcc ttgtcccatt
     8521 tcacgccggt gtgggtgtag gggatggcgt aggtgaacag gaagcctcgg gagatgttgt
     8581 agtcgtcctt ggcctcgatc acct
//
LOCUS       DAOTFD010000096         8597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5813_length_8597_cov_4.232030, whole genome shotgun sequence.
ACCESSION   DAOTFD010000096 DAOTFD010000000
VERSION     DAOTFD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5813_length_8597_cov_4.232030"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1123)
                     /locus_tag="P5079_06660"
     CDS             complement(218..1123)
                     /locus_tag="P5079_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY29705.1"
                     /translation="MIDTPAQDIPGDQEKKYAAKSSVMAAVGLTGLKIAVGLSTGSLG
                     ILAEAAHSGLDLAAAAVTWWAIHVAGKPADRDHRYGHGKVENLSALFETLLLLITCGW
                     IIHSAADRLISRNVEIEVNVWSFLVMFVSIAVDVSRSRLLFRAAKKYNSQALEADALH
                     FSTDIWSSAVVILGLFCVKLSEWLNGYEFLHYADAVAAIVVALIVVKVSVQLGWRTVS
                     ALLDTAPVGMEEQIVRAVERIPGVKNCHQVRLRPSGPVLFVDLHVLVDGRQSIKKAHA
                     LTEEIEQCIQKIVPHSDITVHPEPG"
     gene            1334..2485
                     /locus_tag="P5079_06665"
     CDS             1334..2485
                     /locus_tag="P5079_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRY29706.1"
                     /translation="MSTAEKPIQELIREHRASLPTPYYLIDEPRLLENLKKIRYVRER
                     SGAKSVLALKCFSTWAVFDLMRQYMDGTTSSSLYEARLGYEKFGKEVHAYCVAFSDED
                     VRAVRPFASKVIFNSVSQLKRYAPSLKGLPLGLRVNPGVSHSDFDLADPARRYSRLGA
                     TARAEIEEAAPLISGAMFHCNCENDDFKKFGEILDHIGRGHEPLLRRLEWVSFGGGIY
                     FTKEGYPLDAFCDRLSEFSRRFGVQVYLEPGETAVTRSGFFVTKVLDIVRNEIDIAIV
                     DGAVEPHMLDLLIYRTDAKIEIPQRGGHTYMVAGRTCLAGDVFGTYTFPEKLEVGSLV
                     PFADAAGYSMVKKNWFNGVQMPAIVVRRLDGRVEKVKEFTYEDYLLNLS"
     assembly_gap    2521..2530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2531..>3331
                     /locus_tag="P5079_06670"
     CDS             <2531..>3331
                     /locus_tag="P5079_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRY29707.1"
                     /translation="TAHKCAQNNDILGDICIASRRVKKCDKIIESIKRKDNIKNKSGK
                     IFSRQIDAKDVPALSRLIEETKSSIVINLGTAYVNMSVLEACINTGAAYIDTAIHEEP
                     DKVCEDPPWYANYEWKRRDRCKAKGVTAILGAGFDPGVVNAYVALALKHHFDKIDTID
                     ILDVNAGSHGKFFATNFDPEINFREFKKVWTYIDRKWVSKPCHSEKWIYDFPVVGKQP
                     VYLTGHDELHSLSKNVKANSIRFWMGFSDHYINCFTVLKNVGLLSEKTV"
     assembly_gap    3332..3341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3342..3683
                     /locus_tag="P5079_06675"
     CDS             <3342..3683
                     /locus_tag="P5079_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRY29708.1"
                     /translation="LKACLPDPSSLAPNYTGYTCIGDLIKGEKNGKHKELFIYNSCDH
                     AACYREVEAQAISYTAGVPPVAAAMLVVKGTWDAKTMVNVEELDPDPFLKILDKIGLP
                     TKIEDRTPVSA"
     gene            complement(3711..5843)
                     /locus_tag="P5079_06680"
     CDS             complement(3711..5843)
                     /locus_tag="P5079_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29709.1"
                     /translation="MKLTSLKFRVPDAGARSRTFLWGLTAVLVAAACLAAFFPALHND
                     FVSWDDPDNFLNNPRYRGLGRGQLLWIFTATHMGHYIPVTWLTLAADHALWGMNPFGY
                     HLTSLLFHMANALLVLSILRPLLRLSTEPLGIDVRPNPREMIGLGTAALFFAIHPLRV
                     ESVVWISERRDVVSGFFYLSSLLCYLRYAQTDERTRSGRGWWYGASVGLFLLMVLSRA
                     NNFTLLATLFLLDFYPLRRLRWPPTAATRGVLFEKIPYVLLVLLALASLTVSQNRIGS
                     LQTDVEVMNATMAQTFFGLVFHIKKTFLPLHLAPVYGAVRAGGLAEFLWLNGGLLAAL
                     TAFLVWMRKKWPAAMAAWLHFIVTLLPPHLSGRANSITNDRYGYLASLSASVILLAVL
                     KPVWENNPVSGSPPPGRKTLLVLLSAALLTGLGTLTFRQCRVWRDSETLWRHTVAADV
                     HSPIPYHNLAVTLQRKGQLPQAVSLYTEGLRLFPEAPLLYYGRAGTDLASGKIDEAKK
                     RLRQAVELNPSYGKAYQKLGDIALSLGDLRSAYEHYTKALRLRPTAGRIHSNLGYVCL
                     KLGRNEEAERHLREALQRDPDDAEALNNLGILLLSQGRWPEAEEIFRRGLANSPDSYE
                     LHTHLGNTLLRMGRVSEGWEHLEHALRLNPRSKGAHFYAGAALMDMGRFPEAEKHFEA
                     ALDLDPHFEAARQQLKKFPSAKRGPPGR"
     gene            complement(5850..6998)
                     /locus_tag="P5079_06685"
     CDS             complement(5850..6998)
                     /locus_tag="P5079_06685"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRY29710.1"
                     /translation="MVTQALLFSLAAYPLVLSGRPLAHRWFEFMGVSAGQIGPQKVYF
                     DILLYAVILSLTRNCLSGFFSGIGRTRIVMAASLTAMTVNVLLNYVLIFGKFGLPAMG
                     IRGAAYGTILGGAAGLAMLAAKYFSPAIRTEYRVAESFRFDGHVAGKLLRFGYPSGLE
                     FFLNLLAFNALVLTFHAHDPATATAATIVFNWDMVSFVPLVGVEIGVTSLVGRFMGAG
                     EPETAHRSVMSGLKMGLVYSTVILILFVGFPEELVAVFRPGVPDPTYLRAVPTAVFMV
                     RMASLYVLVEAMIVVFFGALRGAGDTFWAMAMSVSMHWLMVLLLSVGLRALGWSPETA
                     WSVIVFGFLLFSYFVYRRYHGGQWKNIRVVQPEPALPAADVFHEPADL"
     gene            complement(6992..7273)
                     /locus_tag="P5079_06690"
     CDS             complement(6992..7273)
                     /locus_tag="P5079_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29711.1"
                     /translation="MNDVAERPPEKGGVREMIALALPMVVSQACDTVMIFTDRLFLSR
                     LSPELMNAAMGGGLTAFMMMTFFLGLTGYATALVAQYFGREGKNTARGW"
     gene            complement(7421..7828)
                     /locus_tag="P5079_06695"
     CDS             complement(7421..7828)
                     /locus_tag="P5079_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29712.1"
                     /translation="MDKITAMVVDDYESVRNLFKRMSRGLGFELIPVEDGFTALNLLQ
                     STPVDVIFLDVRMPRIDGLETLRRIKKMRPTLPVYMMTGSELDYVLNELSKMGAEGFL
                     FKPFELREVSAILEKVAGPLNKRVGGDLPPKNP"
     gene            complement(7844..>8597)
                     /locus_tag="P5079_06700"
     CDS             complement(7844..>8597)
                     /locus_tag="P5079_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY29713.1"
                     /translation="DKLQEAQENLIQSERLAAIGRISSSLAHEINNPLVAVLGNAQLL
                     LTEIPEDNPWRKDVEVIERCALTCKRLLHEVLGFARRDDFKLVGTNVHWIINKALMLT
                     QQRLANRNVQVEKMLMSDSPLVQASEPHLEQVFVNLIVNAQQAMPNGGTLKIHTYHIP
                     AVASSDPDAPPAPDLIAVEFKDTGTGIIKENLKKILEPFFTTKKPGEGTGLGLSVCND
                     IVKKHHGHLVIESEGKGMGSTITVRLPLSDKK"
BASE COUNT         1732 a         2552 c         2700 g         1593 t
ORIGIN      
        1 cgcgccgaag gcagcggcac ttgcgccgag gcgctctatc cgggttctcc ggaagtctgc
       61 ggttcgcaat tgcgctgcaa gatcgaggcg acctactctt cctgacgggg ggcccgccgg
      121 tccggcgatc gaaggtttaa aagagaacgc cgtttcctta cgacaaggaa acggcgttct
      181 ctttttgaaa cgagggccgt cgcgcggccg atcccgctca tcccggttcg ggatgcacgg
      241 tgatgtcgga gtgggggacg attttttgga tgcattgttc gatctcttcc gtcagggcat
      301 gggctttttt tatgctctgg cggccgtcca ccaacacgtg gaggtcgaca aaaaggacgg
      361 gcccggaggg ccgcagccgc acctgatgac agtttttgac ccctgggatc ctctccaccg
      421 cccggacgat ctgttcttcc attcccacgg gcgcggtgtc cagcagggcg ctcacggtcc
      481 gccagccgag ctgcacgctg accttcacaa cgatcaacgc caccacgatg gccgccaccg
      541 cgtcggcgta gtgcaggaat tcgtaaccgt tcagccattc gctcagcttc acgcagaaaa
      601 ggccgaggat gaccacggcg gaactccaga tgtcggtgct gaaatggagg gcgtcggcct
      661 ccagggcctg gctgttgtat tttttcgccg cgcggaagag caagcgggaa cgggagacgt
      721 ccaccgcgat ggagacgaac atcaccagga acgaccagac gttgacctca atctcgacgt
      781 tcctcgaaat caagcggtcc gcggcgctgt ggatgatcca tccgcaggtg atcaggagca
      841 gcagcgtttc gaacaacgcg gagaggttct ccactttgcc gtgaccgtaa cggtgatcgc
      901 ggtccgccgg tttgccggcg acgtggatgg cccaccacgt cacggcggcg gcggcgaggt
      961 ccaggccgga gtgggccgcc tcggccagaa tgccgaggct cccggtcgac aggccgaccg
     1021 cgatttttag gccggtcagg cccaccgcgg ccatcacgga acttttggcc gcgtattttt
     1081 tttcctggtc gcccggaatg tcctgtgccg gagtgtcgat catcccgcaa atatacaaaa
     1141 ttcggcgggc gcggtcgccc ggggcgattt ttttgatcga cgaggggcgg gctcgccggc
     1201 ccgccgattg cgaggtcgcg atcgccgtcg aaaaaaacgg cgttgaaaaa ataaaatctg
     1261 atataattca cgcgcagcat cgacgcgtcg gcggcgcgcg ttcgccgccg aaaatttttt
     1321 cgccgaggtc gtgatgtcca ccgccgagaa gcccatccag gaactcatcc gcgagcaccg
     1381 cgcgtcgctt ccgacgccct attacctcat cgacgagccc cgcctcctcg aaaacctgaa
     1441 aaaaatacgc tacgtccgcg aacggtccgg cgccaagtcg gtgctggcgc tgaaatgttt
     1501 ctccacctgg gccgttttcg acttgatgcg ccaatacatg gacggcacca ccagcagttc
     1561 gctgtacgag gcccggttgg gatacgagaa gttcggcaaa gaggtgcacg cctactgcgt
     1621 ggctttttct gacgaggacg tgcgcgccgt ccgcccgttc gcttcgaagg tcatcttcaa
     1681 ctccgtctcg cagctcaaac gctacgcccc gtcgctgaag gggctgccct tggggctgcg
     1741 cgtcaatccc ggggtgagcc attcggactt cgacctggcg gaccccgccc gccgttactc
     1801 ccgcctgggg gccaccgccc gcgccgagat cgaggaggcc gctcccctca tcagcggggc
     1861 catgttccat tgcaactgcg agaacgacga tttcaagaag ttcggcgaga tactggacca
     1921 tatcggccgc ggccacgaac cgttgctgcg ccggttggag tgggtgagtt tcggcggcgg
     1981 catctatttc accaaggagg ggtatccgct ggacgcgttt tgcgaccggc tctcggaatt
     2041 ttcccgccgt ttcggcgtcc aggtctacct ggagccgggc gagaccgccg tcacccggtc
     2101 cggtttcttc gtcaccaagg tcttggacat cgttcgcaac gaaatcgaca tcgccatcgt
     2161 ggatggagcg gtggaaccgc acatgttgga tttgttgatt taccgaacag atgctaaaat
     2221 cgaaatcccg cagaggggcg gtcacacgta catggtggcc gggcggacgt gtctggccgg
     2281 cgacgttttc ggcacctaca cgtttccgga gaagttggaa gtgggcagtc tcgtcccgtt
     2341 tgccgacgcg gcgggttaca gcatggtgaa gaagaactgg ttcaacggcg tgcagatgcc
     2401 cgccatcgtg gtgcggcggc tggacggccg cgtcgagaag gtcaaagaat ttacctacga
     2461 ggattatctc ctcaatttgt cctaaggagg tattggtcag tgaaaaagaa cgtgctcctc
     2521 nnnnnnnnnn caccgcccac aagtgcgcgc agaacaacga catcctgggg gatatctgca
     2581 tcgcttcgcg cagagtaaaa aaatgcgaca agatcatcga gagcatcaaa cgcaaagaca
     2641 acatcaagaa taaatccgga aagatttttt cacggcagat cgacgccaag gacgtgcccg
     2701 cgctctcccg cctgatcgag gagacgaagt cctccatcgt catcaacctg ggcaccgcct
     2761 atgtgaacat gtcggtgctg gaggcctgca tcaacaccgg cgccgcctac atcgacacgg
     2821 ccatccacga ggaaccggac aaggtctgcg aggacccacc ctggtacgcc aactacgaat
     2881 ggaagcggcg ggaccgctgc aaagccaagg gcgtcaccgc gattctgggc gcggggttcg
     2941 acccgggcgt ggtcaacgcc tacgtcgcgc tggcgctgaa gcaccacttc gacaagatag
     3001 acaccatcga catcctggac gtcaacgccg ggagccacgg gaagttcttc gccaccaact
     3061 tcgatccgga gatcaacttt cgcgagttca agaaggtctg gacctacatc gaccggaaat
     3121 gggtgagcaa accgtgtcac tcggagaagt ggatctacga ttttcccgtc gtgggcaaac
     3181 agcccgtcta cctcaccggc cacgacgagc tccactccct gtccaagaac gtcaaagcca
     3241 acagcatccg cttctggatg gggttcagcg accactacat caactgcttc accgtcctta
     3301 agaacgtcgg gctcctgtcg gagaagacgg tnnnnnnnnn nctcaaggcc tgtctgccgg
     3361 acccgtcttc cctggcgccg aactacacgg gctacacctg catcggcgac ctgatcaaag
     3421 gcgagaagaa cggcaagcac aaggagctct tcatctacaa ctcctgcgac catgccgcct
     3481 gctaccgcga ggtggaggcc caggccattt cctacaccgc cggcgtgccg ccggtggcgg
     3541 ccgcgatgct cgtggtgaag gggacgtggg acgccaagac catggtcaac gtggaggaat
     3601 tggaccccga cccgttcctg aagatactgg acaagatcgg actgcccacc aagatcgagg
     3661 accggacgcc cgtctcggcc taggcgggaa agggcggccg gagggcgggg ctaccgtccc
     3721 ggcggtccgc gtttggcgga agggaacttt ttgagttgct gccgggccgc ttcgaagtgc
     3781 gggtccaggt cgagggcggc ctcgaaatgt ttttccgctt cggggaaacg gcccatgtcc
     3841 atcaaggcgg ccccggcata gaaatgcgct ccttttgagc ggggattgag ccgcagggcg
     3901 tgttccagat gctcccaccc ttccgacacg cgtcccatcc gcagcagggt gttccccaga
     3961 tgggtgtgca gctcgtagga atccggggag tttgcaagtc cccgcctgaa tatttcttcc
     4021 gcttccggcc accttccttg tgataacaac agaatcccca ggttgttgag cgcctcggcg
     4081 tcgtcggggt cccgttgaag cgcctcccgc aggtgccgtt ccgcctcttc gttccgtccg
     4141 agtttcagac agacataccc cagattggaa tgaatgcggc ccgcggtcgg cctcagtcgc
     4201 agggctttcg tgtagtgttc gtacgcggaa cggaggtcgc cgagggagag ggcgatgtct
     4261 cccagtttct gataagcctt gccgtaggag gggtttagct caaccgcctg ccgcagtcgt
     4321 ttcttcgctt cgtcaatttt tccagaagcc aagtccgtgc ccgcccgccc gtaatagagg
     4381 aggggggctt cggggaacag gcggagtcct tcggtgtaga gggagaccgc ctgcgggagt
     4441 tgccctttcc tttgcagcgt cacggccaga ttgtggtatg gaatcggaga atgcacgtca
     4501 gcagcgacgg tgtgtcgcca gagcgtttcc gagtctcgcc acacccggca ttgacggaac
     4561 gtcagcgtgc ccaggccggt cagcagcgcc gccgagagaa ggacgaggag cgttttcctg
     4621 ccgggagggg ggcttcccga aacggggttg ttctcccata ccggtttcaa taccgccagg
     4681 aggatgacgg aggcggacaa cgaggccaga taaccgtaac ggtcgttcgt gatggagttg
     4741 gcccggcccg acagatgcgg tggcagaaga gtgacgatga aatggagcca ggccgccatc
     4801 gccgccggcc atttttttct catccagacg aggaacgccg tgagcgcggc caacaagccg
     4861 ccgttcagcc agaggaattc cgccaacccg ccggctcgga ccgcaccgta gaccggggcg
     4921 aggtggagcg gcaggaacgt tttttttatg tggaaaacaa gaccgaaaaa tgtctgcgcc
     4981 atcgtggcgt tcatcacttc gacgtccgtc tggagggagc cgatgcggtt ttgtgaaacg
     5041 gtgaggctgg cgagggcgag aaggacgaga aggacgtagg ggattttttc aaagaggacg
     5101 ccccgggtcg cggcggtcgg cggccagcgc aagcggcgga ggggatagaa atcgagcagg
     5161 aagagagtgg ccagcagagt gaaattgttc gcgcgcgaca ggaccatcaa caggaacagg
     5221 cccacggagg ccccgtacca ccatccccgt ccgctgcgcg ttctttcatc cgtttgggcg
     5281 tatcggagat aacagagcaa ggacgagaga tagaaaaaac cggagacgac gtcgcggcgt
     5341 tcggagatcc agacgacgga ttccactcgg agaggatgga tggcgaagaa caacgcggct
     5401 gtcccgagtc cgatcatctc ccggggattc gggcggacat cgatgccgag cggttccgtt
     5461 gagagacgaa ggaggggtct cagtattgaa aggacgagga gcgcgtttgc catgtgaaaa
     5521 agaaggctgg tgaggtggta gccgaacggg ttcattcccc acagcgcgtg gtcggcggcg
     5581 agggtgagcc aggtgaccgg gatgtagtgt cccatgtgag tggccgtgaa tatccaaagg
     5641 agctggcccc ggcccaggcc gcggtatcgg ggattgttga ggaaattgtc cgggtcgtcc
     5701 caactcacga aatcgttatg cagggcggga aaaaatgccg ccaggcatgc cgcggcgacc
     5761 aacacggccg tcagccccca taaaaaggtc cgggagcggg ccccggcgtc gggaacacgg
     5821 aacttgaggg atgtgagttt catcgagggc tagaggtccg ccggttcgtg aaagacgtcc
     5881 gccgccggga gggccggttc cggttggacg acgcggatgt ttttccattg gccgccgtgg
     5941 tatcgccgat agacgaaata ggaaaagagg aggaaaccga aaacgatcac gctccaggcc
     6001 gtttccggag accatcccag ggcgcgcagt cccacggaga ggaggaggac catcaaccaa
     6061 tgcatggaaa ccgacatggc catggcccag aacgtgtcgc cggcgccgcg caacgcgccg
     6121 aaaaagacga cgatcatcgc ctccaccagc acgtacaggg acgccatgcg caccatgaac
     6181 accgccgtgg gaacggcgcg gaggtacgtc gggtccggaa cgccgggccg gaacaccgcg
     6241 accagttctt cggggaagcc gacgaacagg atcaggatga ccgtcgaata gaccagcccc
     6301 attttcaatc cggacatgac cgagcggtgg gcggtttccg gctcccccgc gcccatgaag
     6361 cggcccacca gactggtcac cccgatctcc actccgacga gggggacgaa agagaccatg
     6421 tcccagttga aaacgatggt ggccgccgtg gcggtggccg ggtcatgggc gtggaaggtc
     6481 agcaccaggg cgttgaaggc cagcaggttc aagaaaaact ccagcccgct cggatagccg
     6541 aagcgaagca gttttccggc gacatggccg tcgaaccgga aggattccgc cacgcggtat
     6601 tcggtccgaa tggccggact gaaatatttc gccgccagca tcgcgagtcc cgccgccccg
     6661 cccagaatgg tgccgtaggc cgctccgcgg atgcccatgg ccggcaggcc gaacttgccg
     6721 aagatgagga cgtagttcaa taagacgttg acggtcatcg cggtcagcga ggcggccatc
     6781 acgatgcggg tccgtccgat gccggaaaaa aaaccgctga ggcagttccg ggtcagactg
     6841 aggatcaccg cgtagagcaa tatgtcgaaa tacaccttct gcgggccgat ctgtcccgcg
     6901 ctcaccccca tgaattcgaa ccagcggtgg gccagcggcc ggccggacag gacgagcgga
     6961 taggcggcga gagagaacag cagggcctgc gtcaccaccc gcgcgcagta ttttttcctt
     7021 cccgcccgaa atactgcgcc accagcgccg tggcgtatcc cgtgaggccg aggaaaaaag
     7081 tcatcatcat gaaggcggtc aggccgccgc ccatggcggc gttcatcaat tccgggctca
     7141 gccgcgacag gaacaggcgg tccgtgaaga tcatgaccgt gtcgcatgcc tgggaaacca
     7201 ccatcggcag cgccagggcg atcatctcgc gcactccgcc tttttccggc ggccgttctg
     7261 caacgtcgtt caatcgtcct cccggacagg ggccctctgt gatgggatcg tttcggatgt
     7321 ccccggacgc gcgtccggga aaggttcccg aggatgctag attctatgaa atacggaggc
     7381 ggaaagtcgg aggggagggg tgcggcgcgg gggggacggg ctagggattt ttcggcggga
     7441 ggtcgccgcc cacgcgcttg ttcagcggtc cggcgacttt ttcaaggatg gccgacactt
     7501 cccggagttc aaaaggtttg aacaggaacc cctccgcgcc cattttggac agttcgttca
     7561 agacgtaatc cagttcggac cccgtcatca tgtagacggg gagggtcggc cgcatttttt
     7621 ttatgcggcg gagcgtttcc aggccgtcga tgcgcggcat gcgcacgtcc agaaaaatca
     7681 cgtccaccgg ggtggactgc agcagattca gcgccgtgaa accgtcctcc accgggatca
     7741 gctcgaaccc cagcccccgg ctcatgcgtt tgaaaagatt gcggacgctc tcgtagtcgt
     7801 caaccaccat cgcggttatc ttatccatgg ccgtccgtcc cccctatttt ttatccgaaa
     7861 gtggcagccg cacggtgatc gtggagccca ttcccttccc ttcgctctca atgaccaggt
     7921 gtccgtggtg ttttttcacg atatcattgc agaccgaaag gcccagcccc gttccttccc
     7981 ccggtttttt cgtggtgaag aacggttcca gtattttttt gaggttctcc ttgatgatcc
     8041 ccgtgcccgt gtccttgaac tccacggcga taaggtccgg ggcggggggg gcgtccggat
     8101 cggagcttgc gaccgccggg atgtgatagg tgtggatttt cagcgtgcct ccgttcggca
     8161 tggcctgttg ggcgttgacg atgaggttca cgaagacctg ttccaggtgc ggttcgctgg
     8221 cctggacgag cggggagtcc gacatcaaca tcttttccac ctggacgttc cggttggcca
     8281 gccgctgctg cgtcagcatc agcgccttgt tgatgatcca gtggacgttg gtgcccacca
     8341 gcttgaaatc gtcccggcgc gcaaaaccca gcacctcatg cagaagccgt ttgcaggtca
     8401 aggcgcaccg ctcgatgacc tccacgtctt tccgccaggg attgtcctcg ggtatttccg
     8461 tcaacagcaa ttgggcgttc cccagcacgg cgaccagcgg gttgttgatc tcgtgggcca
     8521 ggctgctgga gatcctcccg atggcggcca accgttccga ttggatgaga ttttcctgcg
     8581 cctcctgcag tttgtcg
//
LOCUS       DAOTFD010000097         8521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5881_length_8521_cov_4.618946, whole genome shotgun sequence.
ACCESSION   DAOTFD010000097 DAOTFD010000000
VERSION     DAOTFD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5881_length_8521_cov_4.618946"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6116)
                     /locus_tag="P5079_06705"
     CDS             complement(<1..6116)
                     /locus_tag="P5079_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY29714.1"
                     /translation="MLKAVAGALSGTFLFSVVLSPVAEANFWQERRESLRRARADGDP
                     HAQYAQLPASLPAADSLLPSIERLPSLPISADRQPVVGGGAFLPGSLPASLASLPAAF
                     GELKKVALPSGKSRGWVVLVQDVHDVSSAQANIAGVLDHLSAASRGSASLLVGIEGVS
                     GGFDLEKFRRLPDASSRRAISEYLLRAGLLTGPEHFGLTAPRAARLWGVEETSLYDRN
                     VRAYQQSLPHRKKARFLQSRWETVLAGARDQKLPPALAEYHRRRMDYVNGRTTPVDYV
                     RALSRLGRPSEGLANVGRFLTVLAVEDALDFSRVEKERTDLIELLSGELSDRDLADLL
                     RESLAYRLGQISYGRYYAYLRDVARRAGIDLESLPAFKDYVRYVLVSEKIDQGGLLEE
                     VGRWESEIVSAQKPSAEARAFLELTDDVLALSKLWSNNLNPREWGRLDERFAEIERVP
                     ARLSAFEAGGADVSAPDDAAWREMLGVFSEFYRAAAERNGVMAERFLAEFDRSVSSAG
                     ADPAFRTAVLVVGGFHAEELQKLLLRRGYACATVTPCVKEVADGTRYLSVFAGGRTPL
                     DRLMLGDRLYLNPPSALAARMPFGFGYLKETVSRTFAALYAGLVAVRGGADLETLRQN
                     LEKELGVRLTVQAVGEASDDYAHFTVRFNDEISFDMVFGRRGTPGEEKVKDEKNHFKE
                     MQLSPANMVDGEVGDFAVYLAPRPMGMFRTILKVVRRHSEKILTGVTFTALLAAFAAF
                     GWWVLGWLTSEVPMMASLQQMFVQPLAYVFRGFMTVSDIGSLSPYVSYVLIAARIGMA
                     VVLLGSLGLAAWLVRRLSAFLRVFQRGWRRALAVPLLAGALVAGITSAGHTALDRFWI
                     PVQQFSNGAQVRLMNIREDQRPMVLRTLEQLPPSFVRGVPSVLMSPLEEGVGGWYETD
                     TRLMALSDDNDAAYQRTTVAHEAGHRDFFGFDEGLAGEWRALHERGKSPGDYVRYYAS
                     LDEDEDYADTAGFYVSDPWKVFDRASRSEVMQGKLVFVARRFVSIASDGSARVRLFGP
                     GGVFVDVPIKVTEGRIDYADLAAAYQAASAGWKVHVSDEEGGAGLSEDTRSPATALAQ
                     GMLPQNFSRLQAFNQKYGKVVGLHVDPAFGRSDKMGRALAVLERALRTLKIDPAAGPV
                     FVHLAESGAHINGQELSARLDGSSSRKALGKEIRRLLSSPEGRATNTIWREALALKKQ
                     ARPQVEELLSVFEKQNQAKLGDPAWAQKYKWAKIAAYDLVETYEESISPGLEFGAEEL
                     AGAIGDLDDMLKEARKLLRDMEQTVPLTGREKGHLAVVGVLSVLGAVFWGFLGAVFVA
                     PLRGLIRLFRRKTRTTDGVLPEAAFDGTAFNGKSAPPRRSSPVPGFDLLWSLSRGIVR
                     VIGGPVKKTVELVLSPYEAVHKKMSAPRAPPKPGSRLWKNIFYAVLSTTVYAISYVTI
                     EGYTKSPEFSNINSLTFAFGREAISLFFAGALLVHGLIFGAKNYRNKIQKLEWKADIR
                     PLLLVSLAKFAVVTPLIYIAYRISSAQTLALLGLASPVFVGIYIGLLLKFPALKKHLG
                     DATKPHLSLGIFISFLVVVSGVALMVFAPGAGNPVIKGSVLVLGIVAINSLIAVFMKK
                     LTHQVNRLILTFLNYVMAVPIIAAVSFTGLFGNIQWSMLMAVFSVWQIWLAGLGIVLG
                     LIFYLKSMKHGSAFFTTLLVDAWDPLITGVFTCLFLDRVTTRFEVAAFLMIFSGVSYI
                     TSRETKLNKGHQYEEAVVAQEGGEEEDVKFPFYRRLLSRIGRALFPPKARERWGVVSA
                     RVAMPFSRLARLFSKKEAAVSAAGGPATVEARAPPPRGLKRIFSAVTDRISLKTLKGV
                     GYALLSGSFYALGYVLLEDYSHMSENVAPLSVAFIRWAMSAIVGVGVAGAWALFSKKE
                     NVKKAFRLDWKKSVRWLIYLNIFTTLLVPFQFLAFRYTTAQVIMILDIAIPLFTGFFV
                     WFMLKSRRLTKWFGLERPGKSTWRSVLGQVAVIGGAVLVMLVGGAEAGGAKTMALMGN
                     LFALVSVVGTAVEFIATERISRR"
     gene            complement(6577..6873)
                     /locus_tag="P5079_06710"
     CDS             complement(6577..6873)
                     /locus_tag="P5079_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRY29715.1"
                     /translation="MSSRVLNEKAPAAVEKTTDSTRSSSGWKTVLFNCNCHTFHEVAL
                     QLTKAIRCTYEKGMHYANLVHYTGCAVVYSGARERCEAVAGILEDIGLRVKVEQ"
     gene            complement(6915..8303)
                     /locus_tag="P5079_06715"
     CDS             complement(6915..8303)
                     /locus_tag="P5079_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRY29716.1"
                     /translation="MDEQQKGASQLNLFSIGEQNKITHVIKRDGRLVAFDKKKIANAI
                     FQAAVSVGGNNRDLADSLADQVAAMMYHTYPHRATPTVQEVQDLIEKVLIENGHARTS
                     KAFILHRIEQQRVASQREQHIVAEDNIPYKVLWKVFAWNVDHECETVEKLNRHMERGT
                     WGQLILDAERLYHEEIQKVAAAILDRPKTRLIIVAGPSSSGKTTTTTKISEKLKEKNL
                     SFVLMNLDNYFRNLEEHPKDEYGDYDFETPQALDLKLINDHLAQLMEGKTIQMPIYNF
                     KTGLREEKTESFRLEKNQILLIDSLHGLYEEMTKSVPADNKFKFYIEAMCQLKDAKGE
                     FVRWADLRLLRRMVRDNWHRSYDPVRTVGHWHYVRRSEMRYIVPFIHTVDYVFNGALP
                     FEIPYHKKYMFKYFDKILATFRNDPKKWDAFMRAQRVHDVLEPLNTPATDDDVPKNSL
                     LREFIGGSSYSY"
BASE COUNT         1768 a         2716 c         2528 g         1509 t
ORIGIN      
        1 cggcgggaaa tcctctcggt ggcgatgaac tcgacggccg tccccaccac ggagacgaga
       61 gcgaacaggt tgcccatcaa cgccatggtc ttcgccccgc cggcctccgc cccgccgacc
      121 agcatcacca gcacggcgcc gccgatcacc gccacctgcc ccaaaacgga acgccacgtg
      181 gatttgccgg gccgttccag gccgaaccat ttggtgagcc gcctggattt cagcatgaac
      241 cagacgaaaa aacccgtgaa gagcgggatg gcgatatcga ggatcataat gacctgggcg
      301 gtggtgtacc ggaaagccaa gaattggaac gggacgagga gcgtggtgaa aatgttgagg
      361 tagatcagcc aacggacgga ttttttccag tccagccgga aagccttttt gacattctct
      421 tttttggaga acagggccca cgccccggcc acgcccacac cgacgatggc cgacatggcc
      481 cagcggatga aagccacgga aaggggcgcc acgttctccg acatgtggga ataatcttcc
      541 agcaggacat agccgagggc gtagaaagaa ccggaaagga gggcatatcc cacgcctttc
      601 aacgtcttga ggctgatcct gtccgtgacg gcggaaaaga tccgtttgag tccccgggga
      661 ggcggcgcgc gggcttcaac ggtcgccggt ccgccggccg ccgacaccgc ggcttccttt
      721 ttagagaaga gccgcgccag gcgggagaac ggcatcgcca cccgggcgga gacaacgccc
      781 cacctttccc gggccttcgg cgggaacaag gcccgaccga tccggctcaa caaccgccgg
      841 taaaagggga acttcacgtc ctcttcttcc ccgccctcct gcgccacaac ggcttcctca
      901 tattgatgtc ccttgttcag cttcgtttcc cgcgaggtga tgtagctcac gccgctgaag
      961 atcatcaaga aagccgccac ttcgaacctg gtcgtcaccc tgtccaaaaa caggcaggtg
     1021 aacacgccgg tgatgagagg gtcccatgcg tcgaccaaca gcgtggtgaa aaaagccgat
     1081 ccgtgcttca tgctcttcag ataaaagatc agccccaata caatacccag cccggccagc
     1141 cagatctgcc aaacggaaaa aacagccatc agcatcgacc attggatgtt cccgaacaag
     1201 cccgtgaagg acaccgcggc gatgatggga acggccatga catagttcag gaacgtgagg
     1261 atgagacggt tcacctgatg cgtcagcttc ttcatgaaaa cggcgatgag ggagttgatc
     1321 gccacgatgc ccaacaccag cacactgccc ttgatgaccg ggttgccggc gccgggagcg
     1381 aacaccatca gcgccacgcc cgagaccacc accaaaaaac tgatgaaaat acccagggac
     1441 aaatgcggtt tcgtggcatc ccccaggtgt tttttaaggg ccgggaactt cagcaacaac
     1501 ccgatgtaaa ttccgacgaa cacgggagac gccagcccca agagcgccag cgtctgcgcg
     1561 gagctgatgc ggtaggcgat gtagatcaac ggcgtgacca cggcgaactt ggccaaactc
     1621 accagcagca acggccggat gtcggccttc cattccaatt tctggatctt gttcctataa
     1681 tttttcgccc cgaagatcag gccgtgcacc aggagcgccc cggcgaagaa cagggagatg
     1741 gcctcccgcc cgaaggcgaa cgtcaaggaa ttgatgttgc tgaattccgg ggatttggta
     1801 tagccttcga tggtgacgta ggaaatcgcg tacacggtcg tggagagaac ggcgtagaag
     1861 atgttcttcc ataaccggct ccccggtttc ggcggcgccc gcggcgcgga catcttcttg
     1921 tgcaccgcct cgtaggggga caggaccagt tccaccgtct ttttcaccgg gccgccgatg
     1981 acccggacga tccccctgga cagggaccag aggagatcaa aaccggggac aggggaggac
     2041 cggcggggcg gggcggattt gccattgaac gccgtgccgt cgaaagcggc ctccggcaag
     2101 accccgtccg tcgtccgcgt cttccgacgg aacaaccgga tcaacccgcg aagaggggcg
     2161 acgaacaccg cccccaagaa tccccaaaaa acggcgccaa gcacggacaa aacacccacc
     2221 accgccaggt gtcccttctc gcggccggtc aacgggacgg tctgttccat gtcccgcaac
     2281 agtttccggg cctccttcag catatcgtcc agatcgccga tggccccggc caactcctcc
     2341 gcgccgaact ccaggccggg agaaatcgat tcctcgtagg tttccaccag atcatacgcc
     2401 gcgatcttgg cccatttgta tttctgggcc caggcgggat cccccaattt cgcctggttt
     2461 tgtttttcaa aaacggagag gagttcctcc acctgcggcc gcgcctgctt cttcagcgcc
     2521 agggcctctc gccatatcgt gttggtcgct cggccttcgg gcgagctcaa gagccggcga
     2581 atctccttcc ccagcgcctt ccgcgaggag gaaccgtcaa gccgcgcgga aagttcctgc
     2641 ccgttgatgt gagcgccgga ctccgccagg tgcacgaaaa ccggaccggc cgccgggtcg
     2701 atcttcaacg tccggagcgc ccgctccaac accgccagcg ccctccccat cttgtccgac
     2761 cggccgaaag cgggatccac atgaagaccg accactttcc cgtatttctg attaaaggcc
     2821 tgcagccggc tgaaattctg aggcagcatc ccctgcgcca acgcggtcgc cggcgagcgg
     2881 gtgtcttcgg agaggccggc gcccccctct tcatccgaca cgtggacctt ccatcccgcg
     2941 gaggccgctt gataggccgc cgccaaatcg gcgtagtcga tccgcccctc cgtcactttg
     3001 atcgggacat cgacgaacac gccgcccggg ccgaagagcc gcacccgggc gctgccgtcc
     3061 gacgcgatcg aaacgaaccg ccgagccaca aacaccagct tcccctgcat cacctccgac
     3121 cgcgacgccc ggtcgaacac tttccacggg tcagacacat aaaagccggc ggtgtcggcg
     3181 taatcctcat cctcgtccag ggaagcgtag taccggacgt aatccccggg ggattttccc
     3241 cgttcatgca acgcccgcca ttcccccgcc aacccctcat cgaacccaaa aaaatcccga
     3301 tggcccgcct cgtgcgcaac cgtcgtccgc tgataggccg cgtcgttgtc gtcggaaagg
     3361 gccatcaacc gggtgtccgt ctcataccaa ccgcccaccc cctcttccag gggcgacatg
     3421 agcacggacg ggacgccccg cacgaaggag ggcggcaact gttcaagggt ccggagaacc
     3481 atcggcctct ggtcctcccg gatgttcatc aggcgcacct gggcgccgtt ggaaaattgt
     3541 tgcacgggga tccagaaccg gtccaacgcg gtgtggccgg ccgaggtgat ccccgccacg
     3601 agcgccccgg ccaaaagcgg cacggccaag gcccggcgcc agccgcgctg aaacacccgg
     3661 agaaacgccg acagccgccg gacgagccag gccgccaacc cgagggaacc caggagcacc
     3721 acggccatgc cgatccgggc cgcaatgaga acgtaggaga catagggaga gagagaaccg
     3781 atgtccgaaa ccgtcatgaa gccccgaaag acgtaggcca acggctgaac gaacatttgt
     3841 tggagagacg ccatcatcgg cacttcggag gtgagccacc ccaggaccca ccaaccgaac
     3901 gcggcaaacg ccgccaggag cgcggtgaac gtcacgccgg tcagtatctt ttccgaatga
     3961 cgccgcacca ccttgagaat cgtgcggaac atgcccatgg gccgcggcgc cagatacacc
     4021 gcgaagtcgc ccacctcgcc gtccaccatg ttggcggggc tcaactgcat ttccttgaaa
     4081 tgattcttct cgtccttcac tttttcttcg cccggcgtgc cccggcggcc gaaaaccatg
     4141 tcaaaagaaa tctcgtcgtt gaaacgcacg gtgaaatgag cgtaatcgtc cgatgcttcc
     4201 cccaccgctt gcacggtcag ccggaccccg agctctttct ccaggttctg ccggagggtt
     4261 tccaggtccg ccccgccgcg aaccgccacc aggccggcat acagcgccgc gaacgtccgg
     4321 gacaccgttt ccttcaggta tccgaacccg aagggcatcc gggcggccag ggccgacggc
     4381 gggttgagat agagacggtc gcccaacatc agccggtcga gcggggtccg gccgcccgcg
     4441 aaaaccgaaa gataacgcgt gccgtcggcg acctccttca cgcagggggt cacggtcgcg
     4501 caggcgtagc ctcgccggag caggagcttc tgcaactcct cggcatggaa tccccccacc
     4561 accaacaccg ccgtgcggaa cgccggatcg gcgccggccg aggagacgga acggtcgaac
     4621 tcggccagga accgttccgc catcacgccg ttccgttccg cggcggctcg ataaaattcc
     4681 gaaaaaacgc cgagcatttc ccgccacgcc gcgtcatccg gggccgaaac atcggcgccg
     4741 ccggcctcaa acgcggacag gcgggccggg acgcgttcga tctccgcgaa ccgttcgtcg
     4801 agccggcccc attccctcgg attcaaatta ttggaccaca acttcgaaag cgccaggaca
     4861 tcgtcggtca actcaaggaa cgcgcgcgcc tcggccgacg gtttctgcgc ggacacgatc
     4921 tccgattccc accgccccac ctcctccagc agcccgccct ggtcgatctt ttcgctgacc
     4981 aggacatagc gcacatagtc tttgaacgcg ggaagcgatt ccaggtcgat ccccgcccga
     5041 cgcgccacgt cgcgcagata ggcataataa cggccgtagg agatctgccc gagacggtag
     5101 gccaggcttt cccgcagcag gtcggcgagg tcccggtcgg aaagctcccc gctcaaaagt
     5161 tcgatgaggt ccgtcctctc cttctccacg cgggagaagt ccagcgcgtc ttccaccgcc
     5221 aacaccgtca ggaaccgccc gacgttggcg agcccttcgg aaggacgacc cagccgcgac
     5281 agggcccgca cataatccac gggcgtcgtg cggccgttga cgtaatccat ccgccgccgg
     5341 tgatattccg ccaacgccgg cggcagcttt tggtcccgtg ctcccgccaa aacggtttcc
     5401 caccgggact ggagaaaccg cgcctttttc cgatgaggca ggctttgctg gtaggcccgc
     5461 acgttccggt catacaagga cgtctcctcg acgccccaga gccgcgcggc gcgcggcgcg
     5521 gtcaggccga aatgttcggg tccggtcagc agcccggcgc ggaggagata ttcggaaatg
     5581 gcccgccgcg aggaagcgtc gggcaaccga cggaacttct cgagatcgaa accgccgctg
     5641 acgccttcga tccccaccag cagggaagcc gatccgcgcg aggccgctga aagatggtcg
     5701 agcaccccgg cgatgttggc ctgcgccgag gagacgtcgt gcacgtcctg gacgagcacc
     5761 acccagcccc gggattttcc cgacggcaac gcgacttttt tcagttcacc gaaggcggcg
     5821 ggaagcgagg cgagggaggc cggaagagaa ccgggaagga acgccccccc gccgaccacc
     5881 ggctgacgat cggcggagat cggcagggac ggaagacgtt caatgctcgg gagaagcgaa
     5941 tcggcggcgg gaagggacgc ggggagttgg gcgtattggg cgtggggatc gccgtcggcg
     6001 cgggcgcggc gaagggactc gcggcgttcc tgccagaaat tggcttccgc caccggcgag
     6061 agcaccacgg aaaaaaggaa cgtccccgac agggcgccgg ccacggcctt gagcaacgaa
     6121 cgacgacttc tggcgatcca aaacccgata atgagggagc ccctttgaaa tctatttggc
     6181 tacaagatag atactaacgg tagagaacaa ttgttacgtt aataatttgt aaccgtattg
     6241 taacaatagt ccttttccac gagagggcca attttcaagg gaagtgctgc ggaagacagc
     6301 gggaggaagc aaaacagcgc gtcgatgcat cgaacagcga caacccgggc gcggagggcg
     6361 gacgggagat tccagcccgc ccccgaaaaa ttataagccc cggaaccgca gggcagcgat
     6421 gaggccgggg cttattgaaa tcgggtgttt ccccctgcgg cgtgacatct caagaataaa
     6481 gttttgcgcc aaatggaacg ttaacggatt gtaacggatt tgtaaattaa atagagggcg
     6541 agggaaggac tgttttttga aaaaatgggg tcggcgctac tgttccactt tgacgcgaag
     6601 cccgatgtct tccaaaatgc ccgccaccgc ctcgcagcgt tccctcgcac cggaataaac
     6661 cacggcgcaa ccggtgtagt gcaccaggtt ggcgtaatgc atccccttct cgtaggtgca
     6721 gcggatggct ttggtgagtt gcagggccac ttcgtggaag gtatggcagt tgcagttgaa
     6781 gaggacggtc ttccagccgg aagacgagcg ggtggagtcg gttgtttttt cgacggcggc
     6841 gggggctttc tcgttcaaaa cgcgggaaga cacgtctttc ctcctcgttc cccggccgcc
     6901 ggccgacgga cggctcagta gctgtaggaa ctcccgccga tgaactcccg caaaagggaa
     6961 ttcttcggca catcgtcgtc ggtggcgggg gtgttgagcg gctcgagcac atcgtgcacg
     7021 cgctgggcgc gcatgaaggc gtcccatttc ttggggtcgt tgcggaacgt ggccagtatc
     7081 ttgtcgaaat acttgaacat gtattttttg tgatacggga tctcgaaggg cagcgccccg
     7141 ttgaacacgt agtccacggt gtggatgaac ggcacgatgt agcgcatctc gctgcggcgc
     7201 acgtagtgcc aatggccgac ggtgcgcacc gggtcgtaac tgcggtgcca gttgtcgcgc
     7261 accatgcggc gcaacagacg caggtccgcc cagcggacga attccccctt cgcgtccttg
     7321 agctggcaca tggcctcgat gtagaacttg aacttgttgt cggccggcac gctcttggtc
     7381 atctcctcgt acagaccgtg gaggctgtcg atgagcaata tctggttctt ctccagtcgg
     7441 aacgattcgg tcttctcctc ccgcaggccg gtcttgaagt tgtagatggg catctggatg
     7501 gtttttccct ccatgagctg ggccaggtgg tcgttgatga gtttcaagtc cagcgcctgg
     7561 ggggtttcga aatcatagtc gccgtattcg tccttgggat gctcctcgag gtttcggaaa
     7621 taattgtcga ggttcatcag gacgaaactg aggtttttct ccttcagctt ctcgctgatc
     7681 ttggtggtgg tggtggtctt gccggaactg gacggccccg ccacgatgat cagccgcgtc
     7741 ttcggacggt ccaggatggc cgccgccact ttctgaatct cctcatgata aagccgttcc
     7801 gcgtcgagga tgagctgccc ccaggtgccc cgctccatgt gccggttgag cttctccacg
     7861 gtctcgcatt cgtggtcgac gttccaggcg aacaccttcc aaaggacctt gtaggggatg
     7921 ttgtcctccg ccacgatgtg ctgttcgcgc tgggaggcga cccgttgctg ttcgatgcga
     7981 tggaggatga aagccttgga ggtgcgggcg tggccgttct cgatgaggac tttctcgatg
     8041 aggtcctgga cttcctgcac ggtgggggtg gcccggtgcg ggtaggtgtg atacatcatg
     8101 gccgcgacct ggtcggccag actgtcggcc aggtcgcggt tgttcccgcc gacgctgacc
     8161 gccgcctgaa agatggcgtt ggcgatcttc ttcttatcga aggccacgag tcggccgtcg
     8221 cgcttgatca cgtgcgtgat cttgttctgt tcgccgatcg aaaacagatt cagttggctg
     8281 gcgccttttt gttgttcgtc catgatgttg aaccctctct cgtcttattc ggaacacacg
     8341 attcaaccgc ttgagcgggc cccgacccca tcaaagagcg gagcggccgg accccctgtt
     8401 tcaagaccag agcagattat taaagaaaaa ttatatcgcg atttcaacct aattttcatt
     8461 ttttcccaca aaaaccctcc cgcgcccgcc acgcccccaa ccccgctgcc gtcccggcag
     8521 g
//
LOCUS       DAOTFD010000098         8437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5960_length_8437_cov_4.248270, whole genome shotgun sequence.
ACCESSION   DAOTFD010000098 DAOTFD010000000
VERSION     DAOTFD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_8437_cov_4.248270"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /gene="nuoL"
                     /locus_tag="P5079_06720"
     CDS             complement(<1..1332)
                     /gene="nuoL"
                     /locus_tag="P5079_06720"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY29717.1"
                     /translation="MLDNVYLIPLLPLAAAFLIIAFGRWLPLEGAWIALAASAYGFGH
                     ALLLLSGLYSTHLPLPMEGLNGRYFEQAVTWFNTGVFRMELGVLVDGLSVMMLLVVTS
                     VSLLVQIYSLGYQRGKPRFGRYYAYLSLFSGSMIVLVLANNFLQFFIGWELVGLCSYL
                     LIGFEFERPEAAYAGRKAFITTKLGDLGFYIGLLLLFSTVGTFNFAILKHEHVAAGLI
                     SPEWATVICLLLFCGAVGKSAQLPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVAR
                     LDFLFALSAPAMAVVAWVGCLTALVAASSALVSNDIKRVLAYSTISQLGYMMLALGVG
                     NTYAGMFHLTTHAFFKALLFLGAGSVIHAVHTNDIWRLGGLSKKMPLTFVTVSCATLA
                     IVGFPGFSGFFSKEAVLEAVHAAGQPALFVLASFTAFLTAFYMCRLFFLTFL"
     gene            complement(1336..1641)
                     /gene="nuoK"
                     /locus_tag="P5079_06725"
     CDS             complement(1336..1641)
                     /gene="nuoK"
                     /locus_tag="P5079_06725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRY29718.1"
                     /translation="MTLQAYLLVGAALFVIGLFGVLARRNVLTVLMSIELIFNAANLN
                     LVAFNRFAHPGQPWGQGFALFIIAIAAAEAVVGLALVLALYRNFRTVLTENFDLLKG"
     gene            complement(1638..2132)
                     /locus_tag="P5079_06730"
     CDS             complement(1638..2132)
                     /locus_tag="P5079_06730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY29719.1"
                     /translation="MIEMAVFALLAVLTIVPAALVVTVKNIFHAALFLVLSLTGVAGF
                     FAMLGADFLFVVQILLYAGGITVILLFVVLLSGSPKDWLIRQMNEQWLGALAASAVFV
                     AVLAGLFQYLPGPESISAVGPTTELLGVLLLREMVLPFEAVSLVLLAALVGAVLFTRR
                     GEGK"
     gene            complement(2129..2647)
                     /locus_tag="P5079_06735"
     CDS             complement(2129..2647)
                     /locus_tag="P5079_06735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRY29720.1"
                     /translation="MFDYFCAIGNMGWAFVKGLGVTFKYLFSPAVTVQYPYEKLTPSE
                     LFRGALAFHPDLCISCDMCVRACPSNCISLEAKRNEQTKKKDLQWYRIDFAKCNYCRL
                     CEEICPTKPKSVHHSREFELTFTSRNDFVVEWRPERPQPKALEPGQEWVKFLTQGRPP
                     GRRRAKSPEGKK"
     gene            complement(2651..>3680)
                     /gene="nuoH"
                     /locus_tag="P5079_06740"
     CDS             complement(2651..>3680)
                     /gene="nuoH"
                     /locus_tag="P5079_06740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRY29721.1"
                     /translation="VARKGWPWWVPDFVRLIVPCSLILIFVQLVPVFVLWWELKVAAH
                     IQVRLGPMHVGLWHGWAQPLADGLKLLLKEDIVPAGADRWVHFLAPIVAFVPAYVCYA
                     PIPFGADMAAADLDIGVLFVLGLSGLTVIGLLMAGWGSNNKYSALGGLRAAAQVVSYE
                     VPRVISVLPVVMWAGSLSLSQIMAAQSGLWGGVIPKWFIFYPVIGQVSFLIYLICAVA
                     ETNRVPFDIAEAESELVSGFHTEYSGMKFAFFFMAEYAYVFLGSALGAVLFLGGAEPL
                     PLMRWLPTYVVFFGKTMFLVFVFIWFRWTFPRLRVDRLMDFCWKFLLPWSLVNVLLAG
                     LVFLVRWG"
     assembly_gap    3681..3690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3691..3883)
                     /locus_tag="P5079_06745"
     CDS             complement(<3691..3883)
                     /locus_tag="P5079_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29722.1"
                     /translation="MSFQTPWAVLGPAVPSQTPEGIRRRYQRSVLLVLFLLGAVVLAS
                     VWLGEPAIRFEALMSRLSEL"
     gene            complement(3880..4989)
                     /locus_tag="P5079_06750"
     CDS             complement(3880..4989)
                     /locus_tag="P5079_06750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY29723.1"
                     /translation="MERLSETEMLLNMGPQHPSTHGVLRVVLKLDGEVVTGAMPDVGY
                     LHRGVEKLSENRLYPQVIVLTDRDDYLCAMLNNWAYCLAVEQLLKVEVPARAEYIRVL
                     VGELNRIASHLVLIGTFGIDIGAFTPFLYAFGREREMILDLLDQVSGARITYNYIRIG
                     GVMADLPAGWTEKAGKFVAHMRACLKEYDDLLTYNPIFLDRTRGVGILSKESAVSYGV
                     AGPNLRASGVPLDLRKMAPYGVYGKFDFEMALGKNGDCFDRYLVRRNEIEQSLRIIEQ
                     ALEALPAGDILGKVPKANIRPPAGEAYAHIEGARGWLGVYLISDGSLQPYRMHVRAPS
                     FISLAVLQEILKGWKVADVVAILGSIDIVLGEVDR"
     gene            complement(5067..5600)
                     /locus_tag="P5079_06755"
     CDS             complement(5067..5600)
                     /locus_tag="P5079_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY29724.1"
                     /translation="MDDAQLVERIAERFGEAREVPLPDPKWLRPDAPQVKVPAERVAE
                     VARYLKNELGFDLLDFLTAVDWLKENRFEMVYCFTKTAEPKSRLFLKADLPREGEPGL
                     PSLAGLYKAADWQEREVFDLFGIRFEGHPHLKRILLWEGYPGHPLRKDYVHVPDKYDN
                     GSEIGLPKSAVPSGEHK"
     gene            complement(5601..6113)
                     /locus_tag="P5079_06760"
     CDS             complement(5601..6113)
                     /locus_tag="P5079_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRY29725.1"
                     /translation="MGIVLEKLPVVCHKVPGGELVLTTADFFIDWARQSSIWPLTFGL
                     ACCAIEMMAAYAARFDFNRFGVIPRPTPRQADCLIISGTVTKKMAPAIERLYDQMPEP
                     RFVISMGACANCGGPYYDSYSVAKGVDKVIPVDVYIPGCPPRPEALHNAVLVLQKKIE
                     DSFVAGSRVK"
     gene            complement(6129..6482)
                     /gene="ndhC"
                     /locus_tag="P5079_06765"
     CDS             complement(6129..6482)
                     /gene="ndhC"
                     /locus_tag="P5079_06765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRY29726.1"
                     /translation="MLRQYFHILLFALIGLVFGVMLIVVSSLVRLRGGRKDLTSYECG
                     MEAVGSPWISPNIRFYVFALFFVIFDAEVLFIYPWAVQFKTLGVVGYVEVVIFVAILF
                     LGLIYAWKKDALKWE"
     gene            complement(6476..7192)
                     /locus_tag="P5079_06770"
     CDS             complement(6476..7192)
                     /locus_tag="P5079_06770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY29727.1"
                     /translation="MTEPVSTGERIREMFSGLAPEYDRFNAWASLGLHRFWRRALAGQ
                     IHAGSRVLDLGAGTGALGAEILSRWGKGSGGGVTAVDFSLEMLTRGRRNNPPDDRLSA
                     VQARGESLPFAAGRFDFVVSAFVLRNLQRAGVLEAVFREAYRVLRPSGRLIFLDLRSP
                     EGTLMKWGHRIYLNTMVPAVGRVLFRDRWPGDYLKDTIRELLSEDRIRERFESAGFGQ
                     VRGQPLWGGVAGLFDGEKAC"
     gene            complement(7274..7453)
                     /locus_tag="P5079_06775"
     CDS             complement(7274..7453)
                     /locus_tag="P5079_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29728.1"
                     /translation="MADTLVVVSKIKKVVKEQGLRTGGDYIEALSKKVEAIITESVNK
                     VKADNKKKTLGAEDL"
     gene            complement(7497..8129)
                     /locus_tag="P5079_06780"
     CDS             complement(7497..8129)
                     /locus_tag="P5079_06780"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRY29729.1"
                     /translation="MPDKPDLKVPDSAVPRLAQYHHALMEMAEGVASSDDLARVTGFS
                     AAQIRKDLTYFGQFGRPGFGYSVPDLKQALSRILGTDKAWSVAVVGTGKALSVFGDLD
                     RFRRHGFRVTACFEKEPDKNGAWDDVPVYPMDKLGDVVRRENIRMAVLAVSEGAAQRT
                     AEILAESGVQAILNLTPARLQLPERVAVRPMDLAMELEKLSYRSGRLGDR"
BASE COUNT         1912 a         2710 c         2435 g         1370 t
ORIGIN      
        1 caaaaacgtg aggaaaaaaa gacgacacat gtagaaagcg gtcaggaacg cggtaaacga
       61 cgccaacacg aaaagggccg gctgaccggc ggcgtgcacc gcctccaaca ccgcctcctt
      121 gctgaaaaac cccgagaacc cggggaaccc cacgatggcc agcgtggcgc acgaaaccgt
      181 cacgaacgtc aacggcattt tcttcgacaa cccgcccaac cgccagatgt cgttggtatg
      241 caccgcgtgg atcacgcttc cggcgcccag gaacaacaga gccttgaaga acgcgtgcgt
      301 ggtgagatgg aacatgccgg cataggtgtt gcccacgccc aaggcgagca tcatgtatcc
      361 gagctggctg atggtggaat acgccagcac ccgcttgatg tcgttggaca ccagcgcgct
      421 ggaagccgcc accagggcgg tcagacagcc gacccacgcc accacggcca tggccggggc
      481 ggacagggcg aagaggaaat cgagacgcgc caccaggaac acgcccgccg ccaccatggt
      541 ggcggcatgg atgagcgcgg acaccggcgt gggcccttcc atggcgtccg gcagccagac
      601 atgcaggggc aactgggccg atttccccac cgccccgcag aacaacagga gacagatgac
      661 cgtggcccat tcgggagaga tcagccccgc ggcgacgtgc tcgtgtttca atatggcgaa
      721 gttgaacgtt ccgacggtgg aaaagagaag gagcagtccg atgtaaaacc ccaggtcccc
      781 cagcttggtg gtgatgaacg ccttgcgtcc ggcataggcc gcctcgggcc gctcgaactc
      841 aaaaccgatc agcagatagg agcagaggcc caccagttcc caaccgatga agaactgcag
      901 gaaattattg gccaggacca gcacgatcat ggatcctgaa aaaagcgaga gatacgcgta
      961 gtaacggccg aaccgcggct tgccgcgctg atatccgagg gaatatatct gcaccagcaa
     1021 cgacaccgac gtcaccacca gcaacatcat cacggaaagg ccgtccacca gcaccccgag
     1081 ttccatgcgg aacacgccgg tgttaaacca cgtgaccgcc tgttcaaaat aacgcccgtt
     1141 caacccttcc atcggcagcg gcagatgggt cgaatagagg ccgctcaaca ggagcagcgc
     1201 atggccgaac ccataggcgc tggccgcgag ggcgatccag gccccctcca acgggagcca
     1261 gcggccgaag gcgatgatca agaacgcggc cgccaggggc aacaggggga tcagatagac
     1321 gttatcgagc atccgtcagc ctttcaacag gtcgaagttt tccgtcaaca cggtcctgaa
     1381 attccggtac agggccaaca ccagcgccag acccaccacc gcctccgccg ccgcgatggc
     1441 gatgatgaac agagcgaaac cctgccccca cggctgcccc ggatgagcga aacgattgaa
     1501 ggcgacgagg ttcaggttgg cggcgttgaa aatgagttcg atggacatga ggaccgtcag
     1561 gacgttccgc cgggccaaaa cgccgaaaag cccgatcacg aaaagcgccg cgccgaccag
     1621 gagataggcc tgtagggtca tttcccctcc ccccgccggg tgaacaggac ggcccccacc
     1681 aaggccgcca gcaacaccag cgacaccgcc tcaaaaggca acaccatctc cctcaacagc
     1741 aacacgccga gcaattccgt ggtgggcccc accgcggaga tcgattccgg cccgggcaga
     1801 tactgaaaaa ggccggccaa aaccgccacg aacaccgccg aggccgccag cgctcccagc
     1861 cactgttcgt tcatctgccg gatgagccag tccttggggc ttccggacaa cagcaccacg
     1921 aacagcaaga tcaccgtgat gccgcccgcg tagagcagga tctgtaccac gaagaggaaa
     1981 tccgcgccga gcatggcgaa gaagccggcc accccggtca acgaaaggac caggaacagc
     2041 gccgcgtgaa aaatgttctt gaccgtcacc accaacgccg cggggacgat ggtcaaaacg
     2101 gccaggaggg cgaaaaccgc catctcgatc atttcttccc ctccggtgat ttcgcccgac
     2161 gccgccccgg cggccggcct tgggtcagga acttgaccca ttcctggccg ggctccagcg
     2221 ctttcggctg gggacgttcc ggcctccatt cgacgacgaa atcgttgcgg ctggtgaacg
     2281 tcaattcgaa ctcacggctg tgatgcacgg acttgggctt ggtgggacag atctcctcgc
     2341 agagccggca gtaattgcat ttggcgaaat cgatgcggta ccactggagg tctttcttct
     2401 tggtctgctc gttccgcttc gcctccaggg agatgcagtt ggacgggcag gcgcgcacgc
     2461 acatgtcgca ggagatacaa aggtccggat ggaacgccaa cgcgccgcga aaaagttccg
     2521 agggcgtcag cttctcatac ggatattgca ccgtgacggc gggagaaaaa agatatttga
     2581 acgtcacgcc cagccccttt acgaaggccc accccatgtt cccgatggcg caaaaataat
     2641 cgaacatgcg tcagccccag cgcaccagga acaccaggcc ggccaacagc acgttgacca
     2701 gcgaccacgg cagaaggaat ttccagcaga aatccatgag gcggtccacc ctcaaccggg
     2761 ggaacgtcca gcggaaccag ataaagacga acaccaggaa catggtcttc ccgaaaaaca
     2821 ccacataggt cggcagccag cgcatcagcg gcaacggctc cgccccgccc aggaacagga
     2881 ccgcccccag cgcgctgccg aggaacacgt aggcgtattc ggccatgaag aaaaaggcga
     2941 acttcatgcc ggaatattcc gtatggaacc cggagaccag ttccgactcc gcctcggcga
     3001 tgtcgaacgg cacccggttg gtctccgcca cggcgcagat gagatagatc aggaacgaga
     3061 cctggccgat gaccgggtag aaaatgaacc atttggggat gaccccgccc cacagccccg
     3121 actgggccgc catgatctgc gaaagagaga ggctccccgc ccacatcacc accggcagga
     3181 ccgaaatgac ccgcggcacc tcataggaca ccacctgggc cgcggcccgc aggccgccga
     3241 gagcggaata tttgttgttc gatccccacc ccgccatcag aaggccgatc accgtcaggc
     3301 cggacaatcc cagcacgaac aacacgccga tgtccagatc ggcggcggcc atgtccgcgc
     3361 cgaacggaat gggcgcgtaa cacacgtagg ccggcacgaa cgcgacgatc ggcgccagga
     3421 aatgcaccca ccggtcggcc cccgccggca cgatgtcctc cttcagcagg agcttgagac
     3481 cgtcggccaa gggctgggcc cacccgtgcc acagccccac gtgcatcggg cccaaccgca
     3541 cctggatgtg ggcggcgacc ttgagttccc accacagaac gaaaacgggc accagttgga
     3601 cgaagatcag gatcagcgaa cacggcacga tcagccgcac gaaatccggg acccaccacg
     3661 gccagccctt gcgggcgacg nnnnnnnnnn cgagttcgga caatcgagac atcagcgcct
     3721 cgaagcggat cgccggttcg cccagccaca cgctggccag aaccaccgcg cccaacagga
     3781 acaacacgag gaggacggac cgctgatacc gacggcggat cccttccggc gtctgactgg
     3841 gcaccgccgg gcccaggacc gcccagggcg tctggaaact caccggtcca cctcccccag
     3901 aacgatgtcg atggaaccga ggatggcgac cacgtcggcc actttccatc ctttcaatat
     3961 ctcctggagc acggccaggc tgatgaaaga cggggcgcgg acgtgcatgc ggtagggttg
     4021 cagactgccg tcggaaatca ggtacacgcc gagccagccg cgcgcgccct cgatgtgggc
     4081 gtaggcctcg cccgccgggg gccggatgtt cgccttcggc actttgccga ggatgtctcc
     4141 cgcgggaaga gcctccagcg cctgttcgat gatgcggagg gactgttcga tctcgttgcg
     4201 ccggacgagg tagcggtcga aacagtcgcc gttctttccg agggccatct caaaatcgaa
     4261 ttttccgtac acgccgtagg gagccatctt ccgcaggtcc agcggcacgc cgctggcgcg
     4321 gaggttgggc ccggcgacac cgtaagaaac ggcgctctcc ttggaaagta tcccgacccc
     4381 cctggtgcgg tcgaggaaaa tggggttgta ggtcaacagg tcgtcgtatt ccttcaggca
     4441 ggcgcgcata tgggccacga acttcccggc cttctcggtc cagccggcgg gcaggtcggc
     4501 catcacgccg ccgatgcgga tgtagttgta ggtgatgcgg gcgccgctca cctggtccaa
     4561 aagatcgagg atcatctcgc gttcccggcc gaaggcgtag aggaacgggg tgaaggcgcc
     4621 gatgtcgatc ccgaacgtgc cgatcaggac caggtgcgaa gcgatccggt tcagctcccc
     4681 caccagcacg cggatgtatt cggcgcgggc cgggacctcc accttcagga gctgttccac
     4741 cgccaggcaa taggcccagt tgttgagcat ggcgcagagg taatcgtccc ggtcggtaag
     4801 gacgatgacc tggggataga gccggttctc cgaaagtttc tccacgcctc ggtgcaggta
     4861 gcccacgtcc ggcatggcgc cggtgaccac ctcgccgtcc agtttcagca ccacccgcag
     4921 cacgccgtga gtggacgggt gctgaggccc catgttgagc agcatctcgg tctccgagag
     4981 gcgctccatc ttggtctcga acgagggccc ggaaaggatg gaatcggccg tggcgttgat
     5041 gacgccggcg ggcgggttca gcgggttcat ttgtgttctc ccgacggaac cgcggatttc
     5101 gggagaccga tctcgctgcc gttgtcgtat ttgtccggca cgtggacgta atccttgcgc
     5161 agcggatggc cgggatatcc ctcccacaac aggatccgtt tcaggtgcgg atggccctca
     5221 aagcggatcc cgaaaaggtc gaagacttcc cgttcctgcc agtcggcggc cttataaaga
     5281 ccggccaacg acggcaggcc gggctcccct tcgcgcggca aatccgcctt caggaaaaga
     5341 cggctcttcg gttcggcggt cttggtgaaa caatagacca tctcaaaacg gttttccttc
     5401 agccaatcca cggcggtgag aaaatccaac aggtcgaaac ccagctcgtt tttcaaatag
     5461 cgggcgacct ccgccacacg ttcggcggga accttgacct gcggggcgtc gggacggagc
     5521 catttcgggt cgggcagcgg gacttcccgc gcctcgccga aacgttccgc gatcctttcg
     5581 acgagctggg cgtcgtccat ttatttcacc cggctgccgg cgacgaaaga gtcttcgatc
     5641 ttcttctgca ggacgaggac ggcgttatgg agggcttcgg ggcggggcgg acagccgggg
     5701 atgtagacat cgacgggaat gaccttgtcc acccctttcg ccacggaata ggaatcgtag
     5761 taaggaccgc cgcagttggc gcaggcgccc atggagatga cgaaacgcgg ttcgggcatc
     5821 tggtcgtaaa ggcgttcgat ggccggggcc atctttttgg tgacggtgcc ggaaatgatc
     5881 aggcagtcgg cctggcgcgg cgtgggccgc gggatcacgc cgaaccggtt gaaatcgaaa
     5941 cgggcggcgt aggccgccat catctcgatg gcgcagcaag ccaaaccgaa ggtcagcggc
     6001 cagatgctgg attgccgagc ccaatcgatg aaaaaatccg cggtggtgag gaccaattcc
     6061 ccccccggca ccttgtggca gacgaccggc agtttttcga ggacgatgcc catcttaaga
     6121 cacccgactc attcccattt caacgcgtct ttcttccagg cgtagatcag tcccaggaaa
     6181 aggatggcca cgaagatcac cacttccaca taccccacca cgccgagcgt cttgaactgg
     6241 accgcccacg gatagatgaa gaggacctcg gcgtcgaaaa tcacgaagaa caaagcgaag
     6301 acgtaaaagc ggatgttggg cgagatccac ggactgccca ccgcctccat gccgcactca
     6361 tacgaggtca ggtctttgcg gccgccgcgc aggcggacca acgaggaaac gacgatcagc
     6421 atcacgccga agaccagccc gatgagggcg aacaaaagga tgtggaaata ctgtctcaac
     6481 atgctttttc tccgtcgaaa agtcccgcca cgccgcccca aaggggctgc ccgcggactt
     6541 gtccaaaacc ggccgattcg aaccgttccc ggatccgatc ctccgacaac agctcgcgga
     6601 tggtgtcctt cagataatcc ccgggccatc ggtcgcggaa gaggacccgc cccaccgccg
     6661 gaaccatcgt gttcaggtag atccggtggc cccatttcat caacgtcccc tccggggaac
     6721 ggaggtccaa gaagatcaga cggccggaag gccgcaaaac ccgataggcc tcccggaaaa
     6781 ccgcctccaa aacgccggcc cgctgcagat tcctcaacac gaaggccgac accacgaaat
     6841 cgaaacggcc ggcggcgaag gggagggact ctcccctcgc ctgaaccgcg gaaagccggt
     6901 cgtccggcgg attgtttcgg cggccgcggg tcaacatctc cagactgaag tcgaccgccg
     6961 tcaccccgcc gccggaccct tttccccagc gcgacaagat ctctgcgccg agggcgcccg
     7021 tcccggcgcc gaggtccagc acgcgggatc cggcgtggat ttgccccgcc aaagcgcgcc
     7081 gccagaaccg atgcaacccc agactggccc aggcgttgaa ccggtcgtat tcgggcgcca
     7141 acccggaaaa catttcccgg atgcgttctc ccgtcgaaac cggttcagtc acccggcccc
     7201 ccgtacaaaa aaccatcctc ccgttccccg gaaggaacgg gagtcctcgc gaaaaaagtt
     7261 cgaaaaacgc ggattaaagg tcttccgcgc cgagggtctt tttcttgttg tccgccttca
     7321 ccttgttcac ggattcggtg atgatggctt ccactttttt ggagagagcc tcgatgtaat
     7381 caccgccggt gcgcagaccc tgttccttca caaccttctt gatcttgctg acaactacga
     7441 gcgtatctgc catggtgtct cctttttatt aggaatgatt accgcaacaa aacgttttat
     7501 cggtccccga gacggccgga ccgataggag agtttttcca gctccatcgc caagtccatc
     7561 ggacggaccg ccacccgttc cggcaattgc aaccgcgccg gagtgaggtt caagatcgcc
     7621 tgaacgccgg attccgccaa aatctccgcg gttctctgcg ccgcgccctc ggacaccgcc
     7681 agcaccgcca tccgaatgtt ttcccgacgg acgacatccc ccaatttatc catcggatag
     7741 accggcacat cgtcccacgc gccgttttta tccggctctt tttcaaaaca cgccgtcacg
     7801 cggaaaccat gccgccgaaa ccgatccaaa tccccaaaaa ccgacaacgc tttccccgtc
     7861 cccaccacgg ccacggacca ggccttgtcc gttcccagta tccgggaaag ggcttgcttc
     7921 aaatccggaa cgctgtaacc gaaccccggc cgaccgaact gtccaaaata cgtcaaatct
     7981 ttgcgtatct gcgccgcgct gaacccggtc actcgcgcca aatcgtccga agaagccacc
     8041 ccttcggcca tttccatcaa cgcatgatgg tattgggcca gcctgggaac cgcggaatcc
     8101 ggaactttaa ggtcaggctt atcaggcatt atataggtat ggttttttca aggagcgaac
     8161 aggcctatta gtgaaaggaa tcacgaactc atccggccca ttcgctgggg aattaaatca
     8221 cttttatcgt tgttttgtca aatattctaa aaaaagtctt ttttatggat ttaacggttc
     8281 atgtctttta aagcggtcat cgttggggaa tccccacaga cggcgcaatc gcggcgacgc
     8341 atgacgcgct gttcacgaaa aaccattttt tcgaggtcca acaacaacag ccggtccacc
     8401 agaagatccg gcatcttcaa aatccatttg atgatct
//
LOCUS       DAOTFD010000099         8433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_5962_length_8433_cov_4.514443, whole genome shotgun sequence.
ACCESSION   DAOTFD010000099 DAOTFD010000000
VERSION     DAOTFD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_8433_cov_4.514443"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5079_06785"
     CDS             complement(<1..320)
                     /locus_tag="P5079_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29730.1"
                     /translation="MEVGLDSFFPLFVNFVLPILAGLVYFAMARYVHHIGPMRTLITG
                     QLTYQGAYWGFLFFGIYLASRPLQILAPHPWPLIVNNVREFFMIALFGPAARLAEALR
                     KRGVP"
     gene            complement(485..1123)
                     /locus_tag="P5079_06790"
     CDS             complement(485..1123)
                     /locus_tag="P5079_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29731.1"
                     /translation="MGKKLWSVFLATVMAAVFSAAFVRAEEGEIFVTDVLVVDNNASV
                     TLNDAIQIRDVKVNKSGDRITLKFPEYVGKSGRAFPQVTVQSKKAYDNIVEAISSGQP
                     SKDKAKTIKFRVEDPQILRSPSRRANVEVTFNDAISMTLGVLKSKREGQPYWIGYPGR
                     RNAETGKYVNQVVIINPKLKKAVEQAILDKFERALSEQGESGGEEGGADSER"
     gene            complement(1130..2320)
                     /locus_tag="P5079_06795"
     CDS             complement(1130..2320)
                     /locus_tag="P5079_06795"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRY29732.1"
                     /translation="MVFAITLFHYFTTSPAFAAFKDYGWGARPSAMGGAYTAISNDAE
                     GTLYNPAGLGRLESRQLSLMYAKPYAGLDLNAGSDGTTSLGMNYLAAVSPLGRFGSLG
                     LTWANFNVSGMYQEDTYALSYGRGLHDLLWPEKDLKVFLGFNVKSLSHKYKLDDRTRQ
                     TEATSSASPFRGGTAQSALSLDMGLLVQPLESFSLGLSGRNLNQPDVGLQQKDIVPRD
                     YRLGFAWTSGDLAFFEDLTPSLEISYRKPSGNDADLRMHFGVESWFGFHTYALRLGGN
                     DRELTFGGGWNKEFSRFGLQLDYAFVWSTLVEDNSGTHRITLSLRQGVAPSGDESMAD
                     VEEVPVEEAPPVHPPARTVKPEQTSPESLQKRPPRRYAPQIEDRPEKESAPAKPIQRT
                     RPRR"
     gene            complement(2538..8402)
                     /locus_tag="P5079_06800"
     CDS             complement(2538..8402)
                     /locus_tag="P5079_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY29733.1"
                     /translation="MTANTSSHITQIQAFNDDGAGGVKTDVSAPVFSLANEPTGLTLS
                     DVYSSSVTLTWGVNDNSSSTRYEVSLSPDGFTSYFSTPVVLAAGLTANTTAIVGLMEG
                     TTYSFRVRAYNGDDVASNFSATTNTVTQTIAPTAVTDLAASVTNATLGAVTLTWTAPS
                     DLPGGVNVSKYIVRYATFTSASVGGTDTWWGLATDVRGEPAPAVPSSTELFVLSGLPG
                     GQSLTFHVRSTDTANNLSAVDSGTEQTVSLPAHVVISKLRYGSGGLDFVELYNPTANP
                     IDLQTLPLNFHRRTSAGGDSNVPLTFVNPTIPSKGFFLIAPDDTVTGGVSPDATTGGA
                     DLYSGSNSVYICSSATALTDIIDVLATGSGTDTNFVDFGDPNAPARIGNPASGQGLMR
                     LPSNGVGHSTDTNKWQSDFVSTATPVAHNSLSGAEPDQGAPAAISDLTALAGAEGIIS
                     LSWTSPGDDGVSGGISGGAFILKYTTMGIINDFSSPPSPAATVNLSTTAAAGSAQVYV
                     LSDLIRGASYWFAIAAQDELANQSVWTSSGTNPAVNTQAFAVAYDAAPSSPTAVTIQS
                     RGPTFVQLTWTAPTPDPGDITGYEIYYDTDAAGAPYDGTGATDGASPISLGSVTNYVL
                     TGLQINATYYIALKTVDAGPHVIRSDFSSEVSTYTELALPGAPATFTGAAQSNTSIWW
                     ECSLVAGATSYELQTSTGGVVVSLAHPTTAYLQTGLTANTSSYIAQVQTRNEAGAGGI
                     TPAAGTPVYTLANQPTALTLSNVHASSVTLTWNANGNSPSTRYEVSLSSDGFTSYFST
                     PVALSANLTANTTALLGLAESTAYSFRVRAYNGDDIPGTMSAATNTVTQTLAPNAVTD
                     LAASVTDAFLGAVTLTWTAPSDAPSGAAASKYIVRYATFTAASVGGANTWWSLATDVP
                     GEPTPAAPSSGELFTLSGLPSGQSVTFHIRSTDTANNLSAVDSGTEQTVSVPAHIMFS
                     QVKRGGTAASSFVELYNPSNSSVNLSAGSVYLHRRSNAGTDVGSRIQALTGTIPAKGF
                     YLIADSTDATSGVALDFEWDTAGDTLSLANVTLYLSQGLLPLIDVIDVLAMGNPSTDV
                     NFIDFGDANAPVRLSTPTSNLAAVRRPAGGAGHATDSNKWQSDFLNNQTPNPRNTSSP
                     AEPNDPPTAPVVTYPNGGESFIQGNGITVTWTAATDPESATLYYDVDYSLNNGGAWVD
                     ISSGVAATSLAWTLPMTTSIQALVRVRAWDGVQFGNFDQSDANFSIVTDTIPPAAISD
                     LAAVAGSAEGQIALSWTSPGDDDISGTLTGGEFLVKYSSVQIIRAADFASPPFAHTAL
                     AISTSGVNNGNAQYRLISNLTPGSTYWFAIKTRDEQLGNYSVWNSSGDNASVNTLAWA
                     AAPDLPPGAPVISAVAAVNRQISVTWSPPSPDPGDIASYTVLWATYSTFNGAGEPNVS
                     STSVSSPALSTAVPANNGYPYYFKVQAVDGPLGGAQAGSASAISAEARPRIRPPTGVA
                     GAHTGSSVNLSWTASPDAGVSNCGGYNLYRSTVSGTGYAYLANLAAAAVSYVDSSSIS
                     ATQTYYYVLRTSDTDSATLAGYAESADSNEAAAQSDVTAPLIAHSPVSFLIFNGSAPL
                     TLSAVAVDYRDAARTLAGTVQSMTLYVGLSTAATAYTTVSFSGSGFGTSVASGTAAVS
                     VDFLRQAASFGGFSYYLTATDGVNTAVSPPAPSVNIVTVDSPAGRDVGPEGGVVTTPG
                     GFVEVVITTNALTGTVSITANPLDSATLPIGLAADGIPIRDSYTGRPLTSGAFGPDGL
                     TFRVAVKIRLKYLDTDGDGYPENPPGGNAYELPNTADFHVNNLKIFWLSGGRWVLMGG
                     KREGDRIVVNTTHFSTYALFPAGASGVKTAERFVTPNHDGVNDYATFGTDAVRVTIYD
                     MTGKEMWSATGDGLTAIQWPGTDSDGNVLESGAYIYKAEGSGGSNTHGVVVIAK"
BASE COUNT         1569 a         2608 c         2746 g         1510 t
ORIGIN      
        1 ggaaccccgc gcttgcgcag cgcctcggcc agtcgcgccg ccgggccgaa cagagcgatc
       61 atgaagaact cgcggacatt gttgacgatc agcggccagg ggtgcggcgc caggatctgc
      121 agagggcggg acgccagata gatgccgaag aaaaggaaac cccagtacgc gccctgatag
      181 gtgagttggc cggtgatgag agtgcgcatg ggtccgatgt gatgcacgta tcgcgccatg
      241 gcgaaataca ccaatcccgc caggatgggc agcacaaagt tcacgaacag cggaaaaaac
      301 gaatccaatc cgacctccac gatgtcaacc gcctgatgtt cccgccgaca acccgccgaa
      361 actctctccc aaacccggcg gaccggacca ctccattttg ccaaaaaccg actgaaaatt
      421 ccggttaaaa caaaggcgtc cctttttaac gggacgcctt gaaaagaatc ccacgccggc
      481 ccgatcagcg ctcggagtcc gctcctccct cttcgccgcc gctctccccc tgctcgctca
      541 aggcgcgctc gaacttgtcg aggatggcct gttccacggc ctttttgagt ttggggttga
      601 tgatgaccac ctggttgacg tatttgcccg tctccgcatt gcgccggccc gggtaaccga
      661 tccaataggg ctggccttcg cgtttgctct tcaaaacgcc gagggtcatg gagatggcgt
      721 cgttgaatgt cacttccacg ttggcgcggc gggaggggct gcggagtatc tgcgggtcct
      781 ccacgcggaa tttgatcgtt ttggctttat ccttggaggg ctggccggag gagatggcct
      841 ccacgatgtt gtcgtaggcc tttttcgact gcaccgtcac ctgcgggaag gcccggccgc
      901 tcttgcccac gtactcgggg aacttcaggg tgatccggtc gccgctcttg ttcaccttga
      961 cgtcgcggat ctggatggcg tcgttcagcg tgacagacgc attgttgtcc accaccaaaa
     1021 cgtcggtcac gaaaatctcc ccttcctccg ccctcacgaa cgcagcggag aaaaccgctg
     1081 ccatcaccgt tgccaaaaaa acgctccaca gtttctttcc cacgtgttgt tacctcctcg
     1141 gtcttgtcct ctgaatcggt tttgccggcg cggactcttt ttccggcctg tcctcgattt
     1201 gcggggcata ccggcgcggc ggccgttttt gaaggctttc cggagacgtc tgttccggtt
     1261 tcaccgtccg tgccggcgga tggacgggcg gcgcttcctc gacggggacc tcttcgacgt
     1321 ccgccatgga ttcatccccg gacggagcca ctccctgccg aaggctcagc gtgatccggt
     1381 gcgtgccgct gttatcctcc accagggtgg accacacgaa cgcgtaatca agctgcaacc
     1441 cgaaacgcga aaattccttg ttccagccgc cgccgaacgt cagctcccgg tcgttcccgc
     1501 ccagccgcag ggcgtaggta tggaaaccga accaggactc cacgccgaaa tgcatgcgca
     1561 gatccgcgtc gttgcccgac ggtttacgat aggatatctc cagtgaaggc gtcaagtctt
     1621 caaaaaacgc cagatcgccg ctcgtccagg caaaacccag ccggtagtcc cgcgggacga
     1681 tgtccttctg ttgcaggccg acgtccggct ggttgaggtt gcggccggag agccccagag
     1741 aaaacgattc cagcggctga accaaaagac ccatatccag cgagagggcc gactgggccg
     1801 tgccgccgcg gaacggggaa gcggacgatg tggcctcggt ctggcgggtg cggtcgtcca
     1861 gcttgtattt atgcgacagg gatttgacgt tgaatcccag gaacaccttc aggtccttct
     1921 cgggccagag aagatcgtgc aacccgcgac cgtaggaaag ggcgtaggtg tcttcctgat
     1981 acatcccgct cacgttgaaa ttcgcccagg tgaggccgag cgacccgaac cggcccaacg
     2041 gcgacaccgc cgccaaatag ttcatcccca acgaggtggt gccgtcggac cccgcgttca
     2101 ggtcgagccc ggcatacggt ttggcgtaca tgagggacag ctgtcggctc tccaaccggc
     2161 ccaaccccgc cgggttatac agggtcccct cggcgtcgtt ggaaatggcc gtgtacgccc
     2221 cgcccatggc ggaaggccgc gcgccccagc cgtagtcctt gaacgccgca aaagcgggtg
     2281 aagtggtgaa atagtggaat agtgtaatgg caaataccat cgaaacacag tggaatgggg
     2341 aaatagtggg atggtgacat agtgaaataa ggacgcgata cgacgccgtc cggacaaaag
     2401 aaatggcgcc ggcaaaatta cacttcaatt ttgccttacc cacttctctt atttcgccgt
     2461 tcactattcc gctattcact ctttttcacc attcgcttct tctatctttt tccgttactt
     2521 cactatttca ctactcacta tttagcaatc accaccacgc cgtgcgtgtt ggatccgccc
     2581 gatccctcgg ccttatagat gtaagcgccg ctctccagga cgttcccgtc cgaatccgtg
     2641 cccggccact ggatggccgt caggccgtcg ccggtggcgg accacatttc cttgccggtc
     2701 atgtcgtaaa tcgtcacccg cacggcgtcg gtgccgaagg tggcgtagtc gttcacgccg
     2761 tcgtggttgg gcgtgacgaa ccgttccgcc gtcttcaccc cggacgcgcc ggccgggaaa
     2821 agagcgtatg tggaaaaatg tgtggtgttc accacgatgc ggtccccttc gcgtttgcct
     2881 cccataagca cccagcggcc gccggagagc cagaagatct tcaggttgtt cacgtggaaa
     2941 tccgccgtgt tcggcagttc ataggcgttc ccgcccgggg gattttccgg ataaccgtcg
     3001 ccgtcggtat cgaggtattt aagacggatc ttcaccgcca cgcggaacgt gagtccgtcc
     3061 ggcccgaacg cacccgaggt gagcggccgg ccggtgtagg aatcccggat gggaataccg
     3121 tcggccgcca gaccgatggg gagggtggcg gaatccaagg ggttggccgt gatggacacg
     3181 gtgccggtga gggcgttggt ggtgatgacc acctccacga atccgcccgg cgtggtcacc
     3241 acgccgcctt ccggcccgac gtcccggccc gcgggggaat ccaccgtcac gatgttcacc
     3301 gacggcgccg ggggcgacac ggcggtgttg accccgtcgg tggcggtgag gtagtagctg
     3361 aacccgccga aagaagcggc ctgccgcaag aaatccacgg acaccgcggc ggtgccgctg
     3421 gccacggagg tgccgaaccc cgatccactg aaagacacgg tggtgtaagc cgtggcggcg
     3481 gtggaaaggc ccacatacag cgtcatggac tgaacggtgc cggccagcgt ccgggcggcg
     3541 tcgcgataat ccaccgccac ggcgctcaac gtgagaggcg cgctgccgtt gaagatgagg
     3601 aaggagacag gcgaatgcgc gatgaggggc gcggtcacgt ccgactgcgc cgcggcctcg
     3661 ttggaatcgg cgctttccgc ataaccggcc agggtggcgg agtccgtgtc ggaggtgcgc
     3721 agcacataat agtaggtctg cgtggcggag atggacgagg aatcgacgta gctgacggcc
     3781 gccgccgcga ggttggccaa atacgcgtaa ccggtcccgc tcacggtgga ccgatacagg
     3841 ttatacccgc cgcagttgga gacgccggca tccggcgaag ccgtccacga caggttcacg
     3901 gacgaccccg tgtgcgcccc cgccacgccg gtcggcggcc ggatacgcgg ccgcgcctcg
     3961 gcggagatcg cgctggcgga accggcctgc gctccgccca gcgggccgtc gacggcctgg
     4021 accttgaaat aatagggata cccgttgttc gcgggaacgg cggtgctcag agccggagag
     4081 gaaacgctgg tcgaactcac attgggttct cccgccccgt tgaaagtgga ataggtggcc
     4141 cacagcacgg tgtaagaggc gatgtcgccg ggatcgggcg acggcggcga ccacgtcacg
     4201 gagatctgcc ggttgacggc ggcgacggcc gaaatgaccg gcgcgcccgg cggcagatcc
     4261 ggcgcggccg cccaggccag cgtgttcacc gaggcgttgt caccgctgga gttccacacg
     4321 gaatagttcc ccagctgctc gtcgcgcgtc ttgatggcga accaatacgt ggaccccggc
     4381 gtcaggttcg agatcaaccg gtattgcgcg ttcccgttgt tcaccccgga ggtggagatg
     4441 gcgagggcgg tgtgggcgaa cgggggcgaa gcgaaatcgg cggcgcggat gatctgcacg
     4501 gaactgtatt tgacgaggaa ctcaccgccg gtgagggtgc cgctgatgtc gtcgtcgccc
     4561 ggcgacgtcc acgacagggc gatctgcccc tcggcggatc ccgccaccgc cgccaggtcc
     4621 gaaatggccg cgggcgggat ggtgtccgtg acgatggaaa aattggcgtc ggactggtcg
     4681 aaattgccga actgcacgcc gtcccaggcc cgcacgcgca ccaaagcttg aatggaggtg
     4741 gtcatgggca acgtccaagc cagagaggtg gccgccacgc ccgaggagat gtccacccac
     4801 gccccgccgt tgttcaagga atagtccacg tcgtaataca gcgtggcgct ttcggggtcg
     4861 gtggcggcgg tccaggtgac ggtgatgccg ttgccctgaa tgaaggactc cccgccgttc
     4921 ggataggtca cgacgggcgc ggtgggaggg tcgttcggct ccgccggaga agaggtgttc
     4981 cgcgggttgg gcgtctgatt gttcaaaaag tccgactgcc acttgttgga gtccgtggca
     5041 tggcccgccc cgcccgccgg tcgacggacg gccgccaggt tgcttgtcgg ggtggacagc
     5101 ctcaccggcg cgttggcgtc gccgaaatcg atgaaattaa cgtccgtact gggattcccc
     5161 atcgccagaa cgtcgatcac gtcgatcagg ggcagcagcc cctgcgaaag atagagcgtt
     5221 acgttcgcca gcgacaacgt atctcccgcc gtgtcccatt cgaaatccaa cgccacaccc
     5281 gaggtggcgt cggtggaatc ggcgatcaga taaaaacctt tcgccgggat ggtgccggtc
     5341 agcgcctgga tccgactgcc gacgtccgtc cccgcgttgc tccgacggtg cagatacacg
     5401 ctgcccgcgc tgagattgac gctgctgttg gaagggttat acagctccac gaaactcgag
     5461 gccgccgtcc ccccgcgttt cacctggctg aacatgatgt gcgccggcac gctcaccgtc
     5521 tgttccgtcc ccgaatccac cgccgaaagg ttgttggccg tgtccgtgga acggatatgg
     5581 aaggtcaccg attgcccgct gggtaatccg ctgagggtaa aaagttcgcc ggaagaaggc
     5641 gcggccggtg tcggctcgcc ggggacatcc gtggccaaac tccaccacgt atttgccccc
     5701 cccacgctgg ccgcggtgaa ggtggcgtaa cgcacgatat acttggaagc ggcggcgccg
     5761 gaaggagcgt ccgacggggc ggtccaggtg agcgtcaccg cccccagaaa agcgtccgtc
     5821 accgaggccg ccaaatcggt caccgcgttc ggagcgaggg tctgcgtcac cgtgttcgtg
     5881 gcggccgaca tcgtcccggg aatgtcgtcc ccgttgtagg cccgcacccg aaaactgtag
     5941 gccgtggact cggccagacc cagcaacgcc gtggtgttgg cggtcaaatt ggccgacagc
     6001 gccacgggcg tggagaaata ggaagtgaac ccgtccgaag acagggagac ttcataccgc
     6061 gtcgaaggac tgttgccgtt ggcgttccaa gtcagcgtca cgctggaagc gtgcacattg
     6121 gacaatgtca gggcggtcgg ctgattcgcc agggtataga ccggcgtgcc ggccgccggc
     6181 gtgattcccc ccgccccggc ctcgttgcgc gtctgcacct gggcgatata ggaagaggtg
     6241 ttcgccgtca gccccgtctg cagatatgcg gtggtgggat gcgccagcga aaccacaacg
     6301 ccgcccgtgg acgtctgcag ttcgtaggaa gtggcccccg ccaccaagct gcattcccac
     6361 caaatggagg tgttcgattg agccgccccc gtgaaggtgg ccggcgcccc gggcagggcc
     6421 agctccgtgt aggtgctcac ttccgaagaa aaatcactcc ggatgacgtg cggtcccgcg
     6481 tccacggtct tcagagcgat gtaataggtg gcgttgatct ggaggccggt gaggacgtag
     6541 ttcgtaacgc tgccgagact gatgggggaa gccccgtccg tggcccccgt accgtcgtaa
     6601 ggcgcgcccg cggcgtcggt gtcgtaataa atctcataac cggtgatgtc gccgggatcg
     6661 ggcgtcggcg ccgtccacgt gagctggacg aacgtggggc cgcgactctg gatggtcacc
     6721 gcggtggggc tggagggcgc cgcgtcgtag gccaccgcaa acgcctgggt gttcaccgcc
     6781 gggttcgtcc cgctggaggt ccacaccgat tggttcgcca gttcgtcctg cgcggcgatg
     6841 gcaaaccaat aactcgctcc gcggatcaag tcgctcaaca catacacctg ggcggagccg
     6901 gcggccgccg tggtggaaag gttcaccgtg gcggccggcg acgggggaga ggagaaatcg
     6961 ttgatgatgc ccatggtggt gtatttgagg atgaacgccc cgccggaaat gccgcccgag
     7021 acgccgtcgt cccccggcga agtccacgac agggaaatga tcccttccgc cccggccagc
     7081 gccgtcaggt ccgagatggc cgccggagcg ccctggtccg gttccgcccc cgacaaactg
     7141 ttgtgggcca cgggggtcgc cgtggaaacg aaatcgctct gccacttgtt ggtgtcggtg
     7201 ctgtgcccca cgccgttgga cggcaggcgc atgagccctt gtccgctggc gggattgccg
     7261 atacgcgccg gggcgttggg atcgccgaaa tccacgaagt tggtatccgt gccgctgccc
     7321 gtggccagca cgtcgatgat gtccgtcaga gcggtggcgg aactacagat atagacggag
     7381 ttgctccccg aataaaggtc ggcgccgccg gtggtggcgt ccggcgacac accgccggtg
     7441 acggtgtcgt ccggcgcgat caggaaaaac cctttcgacg ggatggtcgg attcacgaac
     7501 gtcaagggca cgttggaatc cccgccggcg gaggtgcggc ggtggaaatt caaaggcagc
     7561 gtctgtaaat cgatggggtt cgccgtcggg ttatacaact cgacgaaatc caggccgccg
     7621 gaaccatacc gcagcttgct gatcaccacg tgcgccggga gggacaccgt ctgttccgtc
     7681 ccggaatcca ccgcggagag attgttggcg gtgtccgtgg aacggacatg gaacgtcagc
     7741 gactgtccgc cgggcaggcc gctgagcacg aacagttccg tggaggacgg caccgccggg
     7801 gcgggttcgc cgcgcacgtc cgtggccaat ccccaccacg tgtccgttcc ccccacgctg
     7861 gccgaagtaa aggtggcgta tcggacgatg tattttgaaa cgttcacgcc gcccggcagg
     7921 tccgagggag cggtccaggt cagagtcacg gcgccgaggg tggcgttggt caccgaagcg
     7981 gccaagtccg tcaccgcggt cggcgcgatg gtctgcgtca cggtgttggt ggtggccgaa
     8041 aagttcgagg cgacatcgtc gccgttgtag gcccgcaccc ggaaggaata ggtcgttccc
     8101 tccatcagcc ccacgatggc cgtggtgttg gcggtcagtc cggccgccaa aaccaccggc
     8161 gtggagaaat aggaggtgaa tccgtcgggc gacaaagaaa cttcgtagcg cgtggaggag
     8221 ctgttgtcgt tgacgcccca cgtcaacgtc acgctggagg aatagacatc ggacagcgtc
     8281 aggcccgtcg gctcgttcgc cagggagaac accggcgccg agacgtccgt ctttaccccc
     8341 cccgcgccgt cgtcgttgaa cgcctgtatc tgcgtgatgt gggacgaggt gttcgccgtc
     8401 aaatcggttt cgacgtagaa ggcggtcggg tcc
//
LOCUS       DAOTFD010000100         8371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6012_length_8371_cov_3.729197, whole genome shotgun sequence.
ACCESSION   DAOTFD010000100 DAOTFD010000000
VERSION     DAOTFD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_8371_cov_3.729197"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4384)
                     /locus_tag="P5079_06805"
     CDS             complement(<1..4384)
                     /locus_tag="P5079_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY29734.1"
                     /translation="MKKGLAGHIGGILILALLFSPMTAWGKGTVRLTTTANGDGTVTR
                     KANGASATKVTGASYLEIGSNPSPRAECDGSPNTDGGLGDDIDYNDDGVLDGPCSTVN
                     RYHGRTVSFAEWDITSLPSNATLLNVWVNYSAQESSSTFQFQFKDMSLQPTNLANTAD
                     QLLTDIEGGTAFSGSTNVPSGAFPFPSTFTLNSTARADLSAIVSAQSVTWWALGMATS
                     AAGTAPSVYASAPRNIINSYDAGQSPVPTLVVEYSTPPTPATSLTGIAVGATSITWRF
                     MDNAAGFSEDTGTYLQDGSETILVDMGVQPGAGLVTQSVLPLTPNTQYTMHIHQYNEA
                     GASSSTARSTWTYAAQPTNTTVLEVYATSATISWNANDNPNGTPYLVQVTTDPGGNFS
                     AFITTTSTTTTNPSGGVVTFQVAGLIPNTDNYIRVRARNAATPSVPTPFDATVMATPG
                     VASPGLAAFGAIGTFNITAQWTPGGNPYPADNVYYQAQRASDANFTVINDTQPASPTA
                     NTLSANFEGLQRNTTYWFRVRAFDASMIASSWLVLGGTMTAVSPVTSPIHEAVHQTSM
                     SFKWSDLNNTINSPISYRVELSSVSFASGVIFSSATTSAATPSTTITSTVSAPALVPN
                     TTYYARIFALNSIGAPSAQTNVDSPRGWATNPVPADTFELDGTPWEDFINVKWSNNVG
                     LNNSLLTRYRIDASTDSNFSVYTSTSVSPTWVPPASPVYAGSVSSLFSNTTYYLRIIA
                     TGYNGTNSEPLYITTTTRPRPPTSPVITEVGITSVTVSWQSNNNGPNTVYRVQASSEP
                     GYTVFDTSYTVNTWATFLNLSTNTAHQFRVDVVTIHGDTPTFINESTSTLANIPANVA
                     NPYSNMAATSLRVSWNTNTNPSGTNYLVEATSNTTGLFDGNAPIQGATVTTTYRDFST
                     LPNTIYYFRVLAINDDAIKTDFLTIGSTVSRAAQPSITGFTNTSTSLTPVWSTNNNPA
                     DTQYFIENATNAHNSGWTTNNFWQDTALSTNTGYAFQVKARSRDGVETAYSVKWTTRT
                     LAAVPPMPTVSNGAGSRYALDISFSPGTNPPGDPNATQFAVQVLDGSEEPSHLYMYLN
                     SPYGGTPNEFLLDGSSTNPPSTIWKTLAEWNNGVIVATGLVSGRDYTFGILARNGNSG
                     AIIPSLPNSGIPTAPSLGNNAMTGSGAPAIYLDSRGYRHDDGLEHWFNGSVVPFTAKG
                     SFHYHYKFTQNTSSNADDLTEPGWNGKISTQTGTNLANDLSDGEPTQSSTQPVSGFKA
                     TSEGQWVLYVLGDAYLAAPPLADGLPHPNPQFPAQFFLKLDLSSPTVTLKAQYAAGNP
                     YPIENGVGSADSTPYFYWDEPANGTVGAISPIVGYAVSFSSNVADNPPMSQTAPEWIG
                     NTRNYQVPTPQPYGTYFFKVRALDTAGNWTDPPAEFEYVHNPDTQLPVIVNVNMGVTI
                     IIPGSNVYVSVSVNVVVNVTFSEAM"
     gene            complement(4659..6032)
                     /locus_tag="P5079_06810"
     CDS             complement(4659..6032)
                     /locus_tag="P5079_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY29735.1"
                     /translation="MTQAKPAKILVVDDEPNVGMIFHRVLGELGYDVVSAANGMECLR
                     VLKKQEPDVVFLDLRMPGMDGVETLRRLRETHPHLPVLIMTAYQTVTSAVECMKLGAY
                     DYLIKPLDTEHLKSIVKQALALGEISQRTPAARQTAAKETPLVQDVVAEGPEMKRALA
                     LVEKVAPTDLTVLILGESGTGKEVIAREIHQRSPRRSQPFIVVDCAALPESLIESELF
                     GYEKGAFTGADTSKPGKFESAQNGTLFLDEIGNLPLAIQAKLLRFLQEPTIERLGGRK
                     GPLTLNVRIVAATNADLDAAVARGTFREDLFHRLKVFLLQLPPLRERGPEDLKALVNR
                     IVTSFGRQFGKTGLSLAPETLELLEHYRWPGNIRELQNALRSAALLADNVILPTHVPI
                     SIQSHSKPVPSPKENVMLNDIVQKVEKEYILTNLQRFQGDKLRVAEALGMDVATLSKK
                     LKEYGIE"
     gene            complement(6029..7117)
                     /locus_tag="P5079_06815"
     CDS             complement(6029..7117)
                     /locus_tag="P5079_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29736.1"
                     /translation="MTSAAAPRILIVDDELISRELCLDFLQDGGYADVDTAQNGQEAL
                     ERLEKKSFQLVLSDINMPVMDGLAFLKASRPRYPRTEIILMTAFGGLQSALEALRFGA
                     YDYITKPFTQDALLASVRRCLEKQMLSEQLRSAQDELIQKEKLAAVGSMAGWLAHRMR
                     NPLNVILMCSQYLKSKFPEKDEKHDVSMAIEDKVKILDKMTRDFIEFSRSYTPNLRPE
                     DINSFLDKAAANIASRCKIQEVSLDKKYAAGLPPVPIDKDLLEEVVNNLLDNALEAME
                     GPGAIGLKTERAPDGVVVEISNSGAPVPEDVRERIFEPFFTTKERGTGLGLAIARRVV
                     ESHGGKITLVSEPATAFRLQFPLEKTAP"
     gene            complement(7193..7873)
                     /locus_tag="P5079_06820"
     CDS             complement(7193..7873)
                     /locus_tag="P5079_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY29737.1"
                     /translation="MKKKILVVDDDVQLCQLASDILEEHGYQALTANNTDEGFKKLYE
                     ARPDLIILDVWLPSIGGLEFCRQVRLDEQTRHIPIIMLTVQDKEMDKVMGLEMGADDY
                     MTKPFGQRELLARIKALFRRFDRAMPEAKLLRSGDLLIDMDMHVVKVKNRSVPMTPKE
                     FDLLVTLIQNHRKAMNRQNLLTSVWGYDAPATTGTIDVHIRHLRRKLGSHGKKIKTIL
                     GFGYRFDG"
     gene            complement(7888..>8371)
                     /locus_tag="P5079_06825"
     CDS             complement(7888..>8371)
                     /locus_tag="P5079_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY29738.1"
                     /translation="ADLNNFKSFNDKYGFLRGDQAIKLAAQCILSAVEAGGGEGPSFV
                     GHIGGDDFVLVAHPDRVQKICDDIIGRFDSMIPNLYDESDRRQGHIMAKNRQGQVVQT
                     PLMGIAMVIVNSHDREFEHPGEISSLASELKTYAKSFGKSVHVTERRKPPEERPPTTL
                     "
BASE COUNT         1392 a         2229 c         2967 g         1783 t
ORIGIN      
        1 ccatcgcttc gctgaaggtg acgttcacca cgacgttcac gctgacggag acgtagacgt
       61 tgctccccgg gatgatgatg gtgacgccca tgttgacgtt cacgatgacg ggtagttggg
      121 tgtcggggtt gtggacgtat tcaaactccg ccggcggatc ggtccagtta ccggcggtgt
      181 ccagcgcccg gaccttgaag aagtaggtgc cgtagggctg aggcgtcggg acctgatagt
      241 tgcgcgtgtt gccgatccat tccggcgcgg tctgcgacat gggcgggttg tcggccacgt
      301 tggaggagaa gctgacggcg tagcccacga tggggctgat ggcgccgacg gtgccgttgg
      361 cgggttcgtc ccagtagaag tagggggtgc tgtccgcgga accgaccccg ttttcgatgg
      421 gatagggatt cccggcggcg tactgcgcct tgagggtgac ggtgggcgac gacaagtcca
      481 gtttcaggaa gaactgcgcc gggaactgcg ggttgggatg cggaagcccg tccgccaacg
      541 gcggagccgc caggtacgca tcgccgagca catagaggac ccattgtccc tcggaggtcg
      601 ctttgaagcc ggagacgggt tgggtggaac tttgcgtggg ttcgccgtcg ctcaagtcgt
      661 tggcgaggtt ggtccccgtt tgtgtggaaa tctttccgtt ccagccgggt tcggtcaaat
      721 cgtcggcgtt ggacgaggtg ttctgggtga atttgtaatg gtagtggaaa gaccctttcg
      781 ccgtgaaggg aacgacgctc ccgttgaacc agtgttccag accgtcgtcg tggcgatagc
      841 cccgagaatc cagatagatg gccggggcgc cggaacccgt catggcgttg ttgccgagac
      901 tcggggcggt ggggatgccg ctgttgggga gcgacgggat gatggcgccg ctgttgccgt
      961 tccgcgccag gatgccgaag gtgtagtccc gtccggagac cagccccgtg gcgacgatga
     1021 cgccgttgtt ccattccgcc agcgttttcc agatcgtgga gggcgggttg gtggacgaac
     1081 cgtccaggag gaactcgttg ggggtccctc cgtaggggga gttcagatac atgtagaggt
     1141 gggagggttc ttcggaaccg tccaggactt gcacggcgaa ttgagtggcg ttcgggtccc
     1201 ccggtgggtt cgttccgggc gaaaacgaga tgtcgagggc gtagcgggaa ccggcgccgt
     1261 tgctcaccgt gggcatgggc ggcactgccg ccagggtgcg ggtggtccac ttgacggagt
     1321 aggcggtctc caccccgtcc cggctgcggg ctttcacctg gaacgcgtag ccggtgttgg
     1381 tgctcagggc ggtgtcctgc cagaagttgt tggtggtcca accgctgttg tgggcgttgg
     1441 tggcgttttc aatgaaatac tgtgtgtccg ccgggttgtt gttggtgctc cacaccggcg
     1501 tcaaggaggt ggaggtgttg gtaaacccgg tgatgctcgg ctgggcggcc cgggagacgg
     1561 tgctgccgat ggtcagaaaa tcggttttga tggcgtcgtc gttgatggcc aacacgcgga
     1621 aataataaat ggtgttggga agggttgaga agtccctgta ggtggtggtg acggtggcgc
     1681 cctggatggg cgcgttgccg tcaaagagcc cggtggtgtt gctggtggct tccaccaggt
     1741 agttggtgcc gctgggattg gtgttggtgt tccaggagac gcgcaggctg gtggcggcca
     1801 tgttgctgta cgggttggcg acgttcgccg ggatgttggc cagcgtggag gtggactcgt
     1861 tgatgaacgt gggggtgtcc ccatggatgg tgacgacatc cacccggaac tggtgcgcgg
     1921 tgttggtgct gaggttgagg aacgtggccc aggtgttgac ggtgtaggag gtgtcaaaca
     1981 ccgtgtagcc cggctcggaa ctggcctgca cccgatagac cgtgttcggc ccgttgttgt
     2041 tgctctgcca ggacacggtg acggaggtga tgcccacctc ggtgatgaca gggctggtcg
     2101 gcggccgcgg ccgggtggtg gtggtgatgt agaggggttc gctgttcgtg ccgttgtagc
     2161 cggtcgcgat gatgcgcagg taataggtgg tgttggagaa gagggaggaa acgctccccg
     2221 cgtagaccgg agacgcgggc ggaacccacg tgggcgagac cgaggtggag gtatagaccg
     2281 aaaagttcga atcggtcgag gcatcgatcc ggtagcgggt caacagggag ttgttcagcc
     2341 cgacgttgtt ggaccatttg acgttgatga agtcctccca gggcgtgccg tcgagctcga
     2401 acgtgtccgc ggggacgggg ttcgtcgccc accctctcgg ggagtccacg ttcgtctggg
     2461 cggaaggggc cccgatgctg tttaaggcga agatgcgcgc gtaataggtc gtgttgggaa
     2521 ccagggccgg ggccgaaacg gtggacgtga tcgtggtcga aggcgtggcc gcgctcgtcg
     2581 tggccgacga aaatatcaca ccggaagcga agctgaccga agacagctcc acccggtagg
     2641 agatcgggga gttgatggtg ttgttgaggt cggaccattt gaacgacatg ctggtttgat
     2701 ggaccgcctc gtggatgggg ctggtcaccg gggaaaccgc ggtcatggtg ccgcccagga
     2761 ccaaccagct ggaggcgatc atggaggcgt cgaaagcgcg cacgcggaac caataggtgg
     2821 tgttgcgttg caatccttca aagttggcgc tcaacgtgtt ggcggtgggc gaggccggtt
     2881 gggtgtcgtt gatcaccgtg aaattggcat cggaagcgcg ctgcgcctga taatagacgt
     2941 tgtcggccgg gtacgggttg ccgccggggg tccattgggc ggtgatgttg aacgtgccga
     3001 tggcgccgaa ggcggccagg cccggcgagg ccacgcccgg ggtggccatg acggtggcgt
     3061 caaacggcgt gggcacgctg ggggtggccg cgttacgcgc ccgcacgcgg atgtagttgt
     3121 ccgtgttggg gatgagtccc gcgacctgga acgtcaccac cccgcccgac gggttcgtgg
     3181 tggtggtgga ggtggtggtg atgaaggcgg agaagtttcc gcccggatcg gtggtcacct
     3241 gcactagata aggagtgccg ttggggttgt cgttggcgtt ccaggaaatg gtggcgctgg
     3301 tggcgtagac ctccaggacc gtggtgttcg tgggttgggc ggcgtaggtc caagtggacc
     3361 gggccgtgct gctggaagca ccggcttcgt tgtattgatg gatgtgcatg gtgtattggg
     3421 tgttcggcgt cagaggcagg acgctttggg tgaccaatcc ggctccgggc tgcacgccca
     3481 tatcgaccaa gatggtttcg gagccgtcct ggagataggt gccggtgtct tccgagaacc
     3541 cggcggcgtt gtccatgaac cgccaggtga tggaggtggc gcccacggca atgcccgtga
     3601 ggctggtggc cggggtgggc ggcgtgctgt attccaccac cagcgtgggc accggggact
     3661 ggccggcatc ataggaattg atgatattac ggggggcgct ggcgtaaacc gacggggccg
     3721 tgccggcggc gctggtggcc atgcccaagg cccaccacgt gacggactgc gcggacacga
     3781 tggccgacag gtcggctctg gccgtggaat tgagagtgaa ggtgctgggg aacgggaagg
     3841 cgccggacgg gacgtttgtg gaaccactga acgccgtgcc gccttcgata tcggtcaaca
     3901 gttggtcggc ggtgttggcg agattggtcg gttgcagaga catgtctttg aactggaatt
     3961 ggaaggtgct gctggattct tgggcggaat aattgaccca cacgttcaag agggtggcat
     4021 tcgaggggag ggaggtgatg tcccattccg caaaggaaac ggtgcggccg tgataacggt
     4081 tgacggtgga acagggaccg tcaaggacgc cgtcgtcatt gtagtcgatg tcgtcgccca
     4141 gcccgccgtc ggtgttggga gagccgtcgc attcggcgcg ggggctgggg ttggagccga
     4201 tttctaaata ggaagccccg gtgacttttg tggcggaggc gccgttggct ttacgggtga
     4261 cggtgccatc cccgttggcg gtggtcgtaa ggcggaccgt cccttttccc caagcggtca
     4321 tgggcgaaaa gagaagggcg agaatcaaaa tgccgccgat atggcccgcc agcccctttt
     4381 tcacggtttt tccccttttc ttgccaaaaa tcagcgcata atccgcgctc acctagtttt
     4441 catgcaacac aacggccacc acatccgatg gctttatttt gacccctgtc ttcaaatggt
     4501 tcgtggaaca taaaaagaac atcgccgggc acgatcccgt gccgaccgcg gatcgtcccg
     4561 atcccgagat tcacctcaac gacaaaaaca gcgccaggaa acgcgtaaaa tttactttat
     4621 gcaacggaac gacccgtccc gcggcccccc tcggagcgtc attcgattcc atactccttc
     4681 agcttcttgg aaagcgtggc gacatccatg cccaacgctt cggccacccg gagtttatcc
     4741 ccctgaaaac gttgaaggtt ggtgaggatg tattcttttt ccactttctg gacgatgtcg
     4801 ttcagcatga cgttttcttt gggagacggg accggcttgg agtgcgattg gatgctgatg
     4861 ggcacgtgcg tgggaaggat gacattgtcc gccaggagcg cggcgctccg aagggcgttc
     4921 tgcagttccc ggatgtttcc cggccagcgg taatgttcca ggagttccaa ggtctccggc
     4981 gccaggctga ggcccgtttt tccgaattgc cggccgaaac tggtgacgat gcggttcacc
     5041 agcgctttta aatcctccgg accgcgttcc cgcagcggcg gcagttggag gaggaagact
     5101 ttcagacgat ggaacaggtc ttcgcggaac gtgccgcggg ccacggcggc gtccaggtcg
     5161 gcgttggtgg ccgccacgat gcggacattg agcgtgaggg ggccctttcg ccctcccagg
     5221 cgttcgatgg tgggttcttg caggaaccgc aggagtttgg cctggatggc gagagggagg
     5281 ttcccgatct cgtccaggaa aagggttccg ttctgggccg attcgaactt gccgggcttg
     5341 gaggtgtcgg cgccggtgaa ggcccctttt tcatagccga acagttcgct ctcgatgaga
     5401 gattccggga gggcggcgca gtccaccacg atgaacggct gcgaccggcg cgggctccgc
     5461 tgatgtattt cccgggcgat gacttctttg cccgtgccgc tctcccccag gatcagcacc
     5521 gtgagatcgg tgggagcgac tttctccacc aacgccagcg cccgtttcat ctccggccct
     5581 tcggcgacca cgtcctgcac cagcggggtt tctttggccg cggtctgtcg cgcggccggc
     5641 gtccgctggg agatttcccc caacgccagc gcctgcttta cgatggattt gagatgttcg
     5701 gtgtcgagcg gtttgatgag gtagtcgtac gcgcccagtt tcatgcattc caccgcggac
     5761 gtgacggtct gatacgcggt catgatcagc accggcaggt ggggatgcgt ctccctcagc
     5821 cgccgcagcg tctccacgcc gtccatcccg ggcatgcgga ggtccaggaa gaccacgtcc
     5881 ggttcctgtt ttttcagcac gcggaggcat tccatgccgt tggcggcgga gacgacgtcg
     5941 tagcccagtt cccccagcac gcggtggaag atcatgccta cgttgggttc gtcgtccaca
     6001 acgaggatct tggcgggttt ggcctgggtc atggcgcggt tttctccagc gggaattgaa
     6061 gacggaacgc cgtggccggc tcggagacga gggtgatttt ccccccgtgg ctttccacca
     6121 cgcgtcgggc gatggcaagg cccagtcccg tgccgcgttc cttggtcgta aaaaacggtt
     6181 cgaagatgcg ttcccgcacg tcctccggga cgggagctcc ggagttcgat atttcgacga
     6241 ccaccccgtc cggcgcccgt tccgttttca ggccgatggc gccggggccc tccatggctt
     6301 ccaaggcgtt gtcgagcagg ttgttgacca cttcttccag gaggtccttg tcgatgggca
     6361 ccggcggcaa tccggcggcg tatttcttgt cgagagacac ctcctggatc ttgcagcggg
     6421 aagcgatgtt cgccgcggcc ttgtcgagga aggagttgat gtcttccggc cggaggttgg
     6481 gcgtgtagga gcgggagaat tcgatgaagt cgcgcgtcat cttgtcgagg attttgactt
     6541 tgtcctcgat ggccatggag acgtcgtgtt tttcgtcttt ttccgggaac ttcgatttca
     6601 gatattggga gcacatcagg atcacgttga gcggattgcg catgcggtgg gcgagccagc
     6661 cggccatgga acccaccgcg gccagcttct ccttttggat gagctcgtcc tgggccgacc
     6721 gcagttgttc ggaaagcatc tgtttctcca gacagcgccg caccgaggcc agcaaggcat
     6781 cctgggtgaa gggtttggtg atgtagtcgt aggcgccgaa acgcaacgcc tccagggcgg
     6841 attgcagtcc gccgaaggcg gtcatgagga tgatctccgt tcgggggtat cggggccgcg
     6901 aggccttcag gaaggccagc ccgtccatga cgggcatgtt gatgtcggaa aggaccagtt
     6961 ggaaagattt tttttcaagc cgctccagcg cttcctggcc gttttgggcg gtgtccacgt
     7021 cggcgtagcc gccgtcctgc aagaagtcga gacagagttc ccgggagatg agctcgtcgt
     7081 caacgatcag gatgcggggc gcggcggcgg aggtcatcgg cgcggggttt aggtggtcgg
     7141 ccggcggctc tggggtcttc gccgccggac agcggcgggg atctcgcgga ggtcagccat
     7201 cgaaacggta tccgaacccg aggatggttt tgatcttttt tccgtgggat cccagtttcc
     7261 gccgcagatg gcggatgtgc acgtcgatgg tgccggtggt ggcgggggcg tcgtaccccc
     7321 acaccgaggt gaggaggttc tgccggttca tcgccttgcg gtggttctgg atgagcgtca
     7381 ccaggaggtc gaattctttg ggcgtcatgg ggacgctccg gttcttgact ttgacgacgt
     7441 gcatgtccat gtcgatcagc agatcgcccg atcggaggag tttcgcctcc ggcatggcgc
     7501 ggtcgaaacg gcggaacagg gccttgatgc gcgccaggag ttccctctgg ccgaagggtt
     7561 tggtcatgta gtcgtccgcc cccatctcca gccccatcac cttgtccatc tccttgtcct
     7621 gcacggtgag catgatgatg gggatgtgac gcgtctgttc gtccagccgc acctggcggc
     7681 agaattccag cccgccgatg gacgggagcc acacgtcgag gatgatgagg tccgggcggg
     7741 cctcgtaaag tttcttgaag ccctcgtcgg tgttgttggc ggtgagggcc tgatagccgt
     7801 gttcctccaa aatgtccgat gccagctgac acagttgaac gtcgtcgtcc accaccagga
     7861 ttttcttttt cacggtccct cgctctccta taacgtcgtc ggcggtcgtt cctcgggagg
     7921 cttgcggcgt tccgtgacgt gcacgctctt cccgaaggat ttggcatagg tcttcagttc
     7981 gctggccagg gaactgattt cgccgggatg ttcgaattcg cggtcgtggc tgttcacgat
     8041 gaccatggcg atgcccatca gcggcgtctg gacgacctgg ccctggcggt tcttggccat
     8101 gatgtggccc tgccgccggt ccgactcgtc gtaaaggttg gggatcatgg aatcgaaacg
     8161 gccgatgatg tcgtcgcaga ttttttggac ccggtccgga tgggcgacca ggacgaagtc
     8221 gtcgcctccg atgtgcccga cgaacgaggg gccttccccc ccgcccgcct cgacggcgga
     8281 gaggatgcac tgggccgcca gtttgatggc ctggtcgccg cgcaggaaac cgtatttgtc
     8341 gttgaaactc ttgaagttgt tgaggtcggc g
//
LOCUS       DAOTFD010000101         8350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6035_length_8350_cov_4.899458, whole genome shotgun sequence.
ACCESSION   DAOTFD010000101 DAOTFD010000000
VERSION     DAOTFD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_8350_cov_4.899458"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="P5079_06830"
     CDS             complement(<1..1364)
                     /locus_tag="P5079_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29739.1"
                     /translation="MTGREAGEERPKAGSCEVALVQCPGWGRDCPPYAIAALSAYLRA
                     REIGVRGFDLNNALYHGVPETYRKLWDDKDYYVFWENPSRIAQLVGENPALVDRSVRD
                     ILDSGARVVGFTTHTTSFLLSLELARRVKKEDPSRVVVFGGPQCSRAQAGRHFVKEPC
                     VDAVCLLEGEEVLVDVVRQVRRRGSLDGPIPGVLMRVDDRPVDAGDRPLLEDMDGLPI
                     PDYSDFAEGIVGKKYRQPERLEIFDSRGCVRQCHFCSEWQFWRRFRSMSGDRIFREMT
                     LQMSRYPGVDYFYFIGSLLNGDMRALSRLCDLILENGVKVRFAGQAIVRPDMDRDFLR
                     KLARAGCEWLGVGIESGSERVLKNMNKHFTVANAEQVLKDAHEAGIGLQINILFGPPT
                     ETREDFQETLSFLRRVRPNIDTVLASQSFTVVDKGTMLNTHPEKFGITGVDHHLFWES
                     EDGS"
     gene            complement(1361..3349)
                     /locus_tag="P5079_06835"
     CDS             complement(1361..3349)
                     /locus_tag="P5079_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY29740.1"
                     /translation="MSWPVCRTETLSQGLPPGHCVGFLPKTTRWSGEVRLLGDVVVPS
                     GAVLEVDPGTQIRTAEKPLWKYKFLRSGDRGQLQGNDLAFGRLICWGRLVVRGTTEKP
                     VRFLPEGKTWGGIVLLGRARADMEWVELSHAARAGVLCHDRAHLRVQGGKFSGSETGL
                     SAAGFGSVSVTDSSFFSNGRGILSYDFAAVLAEGDYFFENQWGVRADDASFARMERCS
                     FRNNSEAGLLGQGRAWIDLNTNAFEGHEKIAVFLQDKSHGHARGNRFLHNAVGYEVLD
                     DAHLVSDDDKIGPGSSVGVRGLRRARLRFRRAFLHGHLKEAALQTQDGVRAEMTACRF
                     WENGTAISSQGKSRLRLRECEFQKNASAGKLQDRSVVSVVRSRLSGNKTGLEFCGGAR
                     GSLEENEFRAQSAAALDLAAASRVLCRRNRFDENGVALELKETSRAAMSGNHFLRQGS
                     HGVLVRHRAAVRGRGNLFEDNAQAGVQGLDNARLLLAGNTFQANAVGVGIGPWVAADL
                     RRNVFQGQRREGVSSSSEAPVVVKKNIFSKNATGIGHFEKDRALVKKNSFLKNSYAAG
                     TCGGTAFPRWEENRCVDNGRGLVCRDRSGPFVQKNYFEGADNVALLCVGESVPRVRKN
                     RFVGNKIAVATGASSRPGLDSNRWEKNGTETMEWRGEE"
     gene            complement(3394..4542)
                     /locus_tag="P5079_06840"
     CDS             complement(3394..4542)
                     /locus_tag="P5079_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29741.1"
                     /translation="MKKPNATVKEFIYPKLDRWKDSGLARWPVFTADDEVLRELHFEL
                     TYRCNLKCRICDLWGQERQDSTKVSLEMGADEIKRFVRGSKKLSRLEVLVLSGGEPLL
                     KNDAVELVDFFTAHSPSASIGILSNLSNEELIRARLDEIFSRCAPRLWVGTSMDGIGP
                     VHDKMRGAPGSWDRMVKTIAFLRERYPSVDVSITYTMTPHNYRELTAVHDWAEAHKCG
                     FGAQYVVQKEGTEVFRWTRAQLGEVSRQVDALVDRLCRQADAVGHIYRDEADKLKWLW
                     ARLYYWRTLVDYGFEPKRYFPPCLAGRRYAMLDPWGNVSFCSPHKKKPVGNVRETPFD
                     VLWENDKARRLREHIDAGRCDCWLMCTANPVIDRLLALALPSPVPVAP"
     gene            4852..6447
                     /locus_tag="P5079_06845"
     CDS             4852..6447
                     /locus_tag="P5079_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29742.1"
                     /translation="MKMVRPLLRRHIVSLALRSSVFRSLFASFYAFLLLLLKRRAAAG
                     ALKTLYLRQGLLEPEWIPGASDIDTLWVCPKPDISGTHTGRLLRRLAQLKKLFPFLGE
                     SQVVDEAELSAYLRHGDAKRRTAVQWKPLHGQPLPPGPPTDPAGWEWPWDCWAQAFDA
                     HELLLQCYFSEEDGAATRRLNARKHLLDVHRWFFAMDSGAPPPTREETAHKISARSAA
                     EADPEKSWALPLLQSADLPLGKFPQVLARTMTCLRRRGDNIVSRFPSGKNAPPPPAGR
                     PHDDAWERQAWEKRFRALRERDGEGISGLAWDTVRRATILLRDDLPEDAAAELLKTLR
                     HFKKGDRGLRGPLTVVPEQLFRLRQWSIAEGTPFGPYGLRTDIPAGTSAAWRPSTRPL
                     LRNHFCGQIGLPKISPPPAPVLDRLARQTAADWLLNRRTYPLAALSSPPLYVWQSLLM
                     PALSARLYLFAGIASDPHDLEGALLLCRETFPEDKPWWDALLRTLEVEEPAWDGRAVL
                     ETMEREAAGFEKTAGALLEGLGG"
     gene            complement(6427..7671)
                     /locus_tag="P5079_06850"
     CDS             complement(6427..7671)
                     /locus_tag="P5079_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY29743.1"
                     /translation="MPRKRKPHVLFLMPFLPSPPVDGGKKRVHTILRLLSDRYDFTVL
                     SFINKQEQVWDVPALKKFCKAVYPVLLSPAPVPAAEELFLPEIVKSCYSPAMAASLQT
                     LLRESPPDLVHIEFVQMAYYRRWIRDVPVVFTEHDISNISFFRSYFREMTGWRRWGRV
                     VEWMRLVKYQMDACRGLDGVVTLTPPDTEKLRSFLPDLRVQCIYTGVDLKHFSPGRQN
                     GTSPREAEAHLVYVGHYMHYPNEDAILYFCREIFPLIEKLCPIVHLWVVGSGVTKAVE
                     DLQKNPRIHVTGTVKDVKPYLEKADVFVAPLRLGEGIKGKILEAWAMRLPVVTTPVGA
                     RGLQARPEEHYLLGRNPREFAEKTARLIFDRVLRDRLIRNARKLVETTYDWDRLADQV
                     DDFYKEIMENYRRKKALPAQSL"
     gene            complement(7676..7819)
                     /locus_tag="P5079_06855"
     CDS             complement(7676..7819)
                     /locus_tag="P5079_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29744.1"
                     /translation="MALPTDWRVPVRRGVAVWKLTREVAAIFLRRWTELVLRGAGFRR
                     PRD"
     gene            complement(7832..>8350)
                     /locus_tag="P5079_06860"
     CDS             complement(7832..>8350)
                     /locus_tag="P5079_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29745.1"
                     /translation="AIRFQRCEGEVLRTGDLVFLAALAGGPAVGYVVHRLLWKSRRGP
                     SWRYRTMGDWNFSGDSADGIMVGRVTEIRRRERRFPWAGGRVRIGSLGFLVFAKLLRS
                     ADWVFGNIYRRGFVAVLGVSLRGRGPLRLLARGFLLCLVPVLDGGRDFYWRAVLRLSD
                     GFFEALAPAAAD"
BASE COUNT         1586 a         2873 c         2429 g         1462 t
ORIGIN      
        1 ttggagccgt cctccgattc ccagaacaag tgatgatcca cgccggtgat cccgaatttt
       61 tccggatggg tgttgagcat cgttccctta tccaccaccg tgaacgactg gctggccagg
      121 acggtgtcga tgttcggccg cacgcgccgg aggaacgaca gggtttcctg gaaatcctcg
      181 cgggtctcgg tcggcggccc gaacagaatg ttgatctgca ggccgatgcc ggcctcgtgg
      241 gcgtccttca ggacctgctc ggcgttggcc acggtgaaat gcttgttcat gtttttcagg
      301 acacgttccg agccgctctc gatgccgacg cccagccact cgcagccggc gcgggcgagt
      361 ttccggagga agtcccggtc catgtccggc cggacgatgg cctgccccgc gaaccgcacc
      421 ttgactccgt tctccaggat caaatcgcac aagcgcgaga gggcgcgcat gtcgccgttg
      481 aggagcgacc cgatgaaata aaaatagtcc accccgggat aacggctcat ctggagcgtc
      541 atctcccgga agatgcgatc cccgctcatc gaacggaacc gccgccagaa ctgccattcg
      601 ctgcagaaat ggcactgccg gacgcacccc cggctgtcga agatctccag ccgttccggc
      661 tggcggtatt ttttcccgac gattccctcc gcgaaatcgg aatagtccgg gatgggaagt
      721 ccgtccatgt cttccagcag ggggcggtcg ccggcgtcca cgggacggtc gtccacgcgc
      781 atcaagacgc cggggatggg gccgtcgagg gaaccccgcc gccttacctg gcggaccacg
      841 tccacaagga cctcctcccc ttccagcagg cacacggcgt ccacgcacgg ctccttgacg
      901 aaatgccggc cggcctgggc gcgggaacac tgcggcccgc cgaacacgac gacgcgggac
      961 gggtcctctt ttttgacgcg ccgggccaat tccagactca agagaaacga ggtggtgtgc
     1021 gtggtgaaac cgacgacgcg cgcgccgctg tccaggatgt cccggaccga ccggtcgacg
     1081 agggccgggt tttcgccgac gagctgcgcg atgcggctcg ggttttccca gaacacgtaa
     1141 tagtccttgt cgtcccacaa cttgcgatag gtctcgggaa cgccgtggta gagcgcgttg
     1201 ttcaaatcga agccccgcac gccgatctct cgcgcgcgca gatacgccga caacgccgcg
     1261 atggcgtacg gcggacagtc ccgcccccag ccggggcact ggaccagcgc cacctcacag
     1321 gaaccggctt tcggccgctc ctcgcccgcc tctcttcccg tcattcctct ccccgccatt
     1381 ccatggtttc ggttccgttt ttctcccacc ggttggaatc cagccccggc cgggacgagg
     1441 ccccggtggc caccgcgatc ttgttcccga cgaaccggtt cttcctcacc cgcgggacgg
     1501 actcgccgac gcacaggagc gcgacgttgt ccgccccctc aaaatagttc ttttgcacga
     1561 acgggcccga acggtcccgg cagaccagcc cccgtccgtt gtccacgcag cggttttcct
     1621 cccagcgcgg aaaagccgtc cctccgcacg tgccggcggc ataggagttc ttcaagaaac
     1681 tgtttttttt caccagggcg cggtcctttt cgaagtgtcc gatgccggtg gcgttcttgg
     1741 aaaaaatgtt tttcttcaca acgaccggag cctcgctgga ggaactcacc ccctcgcggc
     1801 gctggccctg aaacacgttg cgccggaggt ccgcggcgac ccacgggccg ataccgacgc
     1861 cgacggcgtt cgcctggaag gtgttgccgg ccagcagcag ccgggcgttg tccaaccctt
     1921 gaacgcccgc ctgggcgttg tcctcgaaca gattcccccg gccccgcacc gccgcgcggt
     1981 gccgaacgag aacgccgtgc gacccctgcc ttaaaaaatg attccccgac atcgccgccc
     2041 gcgacgtctc tttcaactcc agagcgaccc cgttctcatc gaaacggttc cggcggcaga
     2101 ggaccctcga cgcggcggcc aggtccagcg cggcggccga ctgggcccgg aactcgtttt
     2161 cctccaaaga accgcgggcc cccccgcaga actccaggcc cgttttgttt ccggacaacc
     2221 gggaacggac gacggagacg acggagcggt cctgcaactt gccggcggag gcgttcttct
     2281 ggaactcaca ctcccgcaaa cgaagacggc ttttcccctg agaggagatc gccgtcccgt
     2341 tctcccaaaa acggcacgcc gtcatctcgg cgcggacgcc gtcctgggtc tgaagcgccg
     2401 cctctttcaa atgcccatgg aggaacgcgc gccggaaccg aagccgggcg cggcgcaagc
     2461 cccgcacgcc gacggaagac cccggaccga tcttatcgtc gtcggacacc agatgggcgt
     2521 cgtccaggac ctcatatccc accgcgttat gcagaaacct gttgcctcga gcgtggccgt
     2581 gggacttgtc ctgtaggaaa acggcgatct tttcgtgacc ctcgaaggcg ttggtgttga
     2641 ggtctatcca ggcccgcccc tgtcccagaa gtcccgcctc gctgttgttc cggaaactgc
     2701 atcgctccat ccgcgcgaag gaggcgtcgt cggcgcggac cccccactgg ttctcaaaaa
     2761 aatagtcccc ctccgcgaga acggcggcga agtcgtaact caatatcccg cgcccattgg
     2821 aaaaaaaaga actgtccgtc accgagacgc tgccgaaccc ggccgcggag agaccggtct
     2881 cgcttccgct gaacttccct ccctgcaccc gcagatgcgc gcggtcatgg cacagaaccc
     2941 cggcccgcgc ggcatgggac aactccaccc attccatgtc ggcccgcgcc cggcccaaaa
     3001 ggacgatgcc gccccacgtt ttgccctccg gcaggaaccg aaccggcttc tccgttgtgc
     3061 cccggacgac gaggcgtccc caacaaatca agcggccgaa ggcgaggtcg ttgccctgga
     3121 gctgtccgcg gtcgccggaa cggaggaatt tgtatttcca aagaggcttt tccgccgtac
     3181 gtatctgggt tcccggatcg acctcgagaa cggcaccgga gggcaccacc acgtctccca
     3241 ggagacgcac ctccccgctc cagcgggtcg tcttgggcaa aaaaccgaca cagtggcccg
     3301 ggggaagtcc ctgggaaaga gtctccgtgc gacaaaccgg ccaggacaag ggctcctcga
     3361 cgctcttgaa aatccccccc cgcgccatgt ccgtcaagga gcgaccggaa cgggcgacgg
     3421 cagcgccaag gcgagcagac ggtcgatcac cggattggcc gtgcacatca gccagcagtc
     3481 gcaccgaccg gcgtcgatat gctccctcaa ccgacgggcc ttgtcgttct cccacaaaac
     3541 gtcgaacgga gtctccctca cgttccccac gggttttttc ttgtgcgggg aacaaaaaga
     3601 aacgttgccc cacggatcga gcatggcgta tcgccggccg gccaaacacg gaggaaagta
     3661 gcgtttcggt tcgaaaccgt agtccaccag cgtccgccaa taataaagcc gcgcccacag
     3721 ccatttcaat ttgtcggcct cgtcccgata aatgtggccg acggcgtccg cctgtcgaca
     3781 caaccgatcc acgagggcgt ccacctgccg ggacacctcc cccaactggg cccgcgtcca
     3841 gcggaacacc tccgtccctt ctttttggac gacgtattga gcgccgaagc cgcacttatg
     3901 agcctcggcc cagtcgtgga ccgccgtgag ctcccggtaa ttgtgcggcg tcatcgtgta
     3961 ggtgatgctg acgtccaccg acgggtagcg ttccctcaag aaggcgatgg tcttcaccat
     4021 ccggtcccag gaccccggcg cgccccgcat tttgtcgtga acggggccga tgccgtccat
     4081 ggaggtgccg acccacaaac ggggagcgca tcgggaaaat atctcgtcga gccgggcgcg
     4141 gatgagctcc tcgttggaaa ggttgctcag gatgccgatg gacgccgacg gtgaatgcgc
     4201 ggtgaaaaaa tcgacgagct ccacggcgtc gttcttgagc agaggctctc cgccgctcaa
     4261 gacgaggacc tcgaggcggg acagcttctt cgatccgcgg acgaaccttt tgatctcgtc
     4321 ggcccccatc tccagcgaaa ccttcgtgct gtcctgccgt tcctgtcccc aaagatcgca
     4381 gatccggcat ttcaagttgc accggtaggt caactcaaag tgcagttccc gaaggacctc
     4441 gtcgtcggcc gtaaagacag gccatcgagc aagaccggag tctttccaac ggtccagttt
     4501 cggatagata aattctttaa cggtggcgtt tggttttttc attggaaatc aaccggtcgg
     4561 cacggacggc agaggcgaga aaataacgcg tcgtatcgga aaaatctcgg cctaaaaaac
     4621 ttgaaagctg gcttgaacat gggttcccct tagttacaat agcgccccaa aaagccagct
     4681 cccaaacgtg ctcgtatcgc tgaaaaatgc gggtttattg tactatgccc cccactcctt
     4741 gtcaaccttg cgcgaatatc attttcatgc taatttctag agtcgaaact gatttcgcat
     4801 tgccggattt ccttgtattt ttctcccgtt ccgggtatcc tttctccaga catgaaaatg
     4861 gtccgacccc tcctccggcg tcacatcgtt tccctcgcgt tgcgttcgtc cgtttttcgt
     4921 tccctgttcg cttcgttcta cgcattcctc cttctccttt tgaagcgccg ggccgccgcc
     4981 ggcgccctga aaactctcta tcttcgccag ggactcctcg aaccggaatg gatcccgggc
     5041 gccagcgaca tcgacaccct atgggtatgt ccgaaaccgg acatctccgg gacgcacaca
     5101 ggccgcctcc tacgccggct ggcgcaactc aagaaactgt tccccttcct gggagaatcc
     5161 caggtggtgg acgaagccga actttccgcc tatcttcgtc acggcgacgc caaacgccgg
     5221 accgccgtcc aatggaaacc cctccacggc caaccgctgc cgccgggacc gccgacggat
     5281 ccggccggct gggaatggcc ctgggattgt tgggcgcagg cgttcgacgc ccacgaactc
     5341 ctcctgcaat gctatttttc cgaggaagac ggcgcggcga cccgtcggct gaacgcccgc
     5401 aaacacctgc tcgatgtgca ccgctggttc ttcgccatgg attccggcgc cccgccgccg
     5461 acccgggaag aaaccgccca taaaatctcc gcccggtccg ccgccgaggc ggaccctgaa
     5521 aaaagctggg ctcttccgct ccttcagtcc gcggacctcc ctttggggaa attcccccag
     5581 gtcctggcgc gcaccatgac gtgcctgcgc cgccggggag acaacatcgt ctcacgtttt
     5641 ccgtcgggaa aaaacgcccc cccgccgccg gcaggccgcc cccacgacga cgcctgggaa
     5701 cggcaggcgt gggaaaaacg gttccgtgcc ctgcgggaac gggacggcga ggggatctcc
     5761 ggcctggcct gggacacggt gcgccgggca acgatcctgt tgcgggacga cctccccgag
     5821 gacgcggcgg cggaacttct gaaaacactg cggcatttca aaaaggggga ccgcggactg
     5881 cggggccccc tcacggtggt cccggaacaa ctcttccgtc tccgtcaatg gagcatcgcc
     5941 gaaggaaccc ctttcgggcc ctacggtctc cggacggaca tccccgccgg gacgtcggcg
     6001 gcgtggcggc cgtcgacgcg cccgctgctc cgaaaccatt tttgcggcca gatcggcctg
     6061 cccaaaatat cgccgccgcc cgcgcccgtg ctcgaccggc tcgcccgcca aacggccgcc
     6121 gattggctcc tcaaccgacg gacctatcct ctcgcggccc tgtccagccc gccgctctac
     6181 gtgtggcaat ccctcctgat gccggctctt tcggcccggc tctatctgtt cgccggcatc
     6241 gcaagcgacc ctcacgacct ggagggcgcc ctgcttctct gccgggagac gtttcccgaa
     6301 gacaaaccct ggtgggacgc gctgctgcgg acgttggagg tggaggagcc cgcgtgggac
     6361 gggcgggcgg tgctggagac gatggaacgg gaggcggccg gcttcgaaaa aacggccggg
     6421 gcgctgttag agggactggg cgggtaacgc ttttttccgt cgataattct ccattatttc
     6481 cttgtaaaaa tcgtccacct ggtcggcgag ccggtcccaa tcgtaggtcg tctccaccaa
     6541 cttccgcgcg ttccgtatca accggtcgcg caggacccgg tcgaaaatca gccgcgccgt
     6601 tttttccgcg aactcccgcg gattccgtcc caagaggtaa tgctcctccg gccgggcctg
     6661 cagcccgcgg gcgcccacgg gcgtggtcac caccgggagc cgcatggccc aggcctccaa
     6721 tattttcccc ttgatcccct cccccagccg caacggcgcc acaaaaacgt ccgccttctc
     6781 cagatagggt ttgacgtcct tgaccgttcc ggtcacgtgg atccgcgggt tcttctgcag
     6841 gtcctccacc gctttggtca cgccggagcc gacgacccac agatggacga tggggcagag
     6901 cttttcgatg agcggaaaga tctccctgca aaaatagagg atggcgtcct cgttgggata
     6961 atgcatgtaa tggcccacgt aaaccaggtg cgcctccgcc tcccggggac tcgtcccgtt
     7021 ctggcggccc ggcgaaaaat gtttcaggtc cacccccgtg tagatgcatt ggacccgcag
     7081 gtccggaaga aaacttctca acttctccgt gtccggaggc gtcaaggtca ccacgccgtc
     7141 cagcccccgg caggcgtcca tctggtattt caccagccgc atccactcca cgacccttcc
     7201 ccaccgccgc cagcccgtca tctcccggaa atacgaccgg aaaaaagaga tgttgctgat
     7261 gtcgtgttcc gtaaagacga ccgggacatc ccttatccac cgccgataat acgccatttg
     7321 gacgaattcg atgtgcacca ggtccggcgg agattcccgc agcagcgtct gcagggaagc
     7381 cgccatggcc ggggaataac agctcttgac gatctcggga aggaaaagct cctccgccgc
     7441 cgggaccggc gcggggctga gcaggacggg atagaccgct ttgcagaatt ttttcagcgc
     7501 gggcacgtcc cagacctgct cctgtttgtt gatgaaggaa agcaccgtga agtcgtatcg
     7561 gtcggagagg agccggagga tggtatggac gcgcttcttg ccgccgtcga cgggcggcga
     7621 gggcaggaac ggcatcagga aaaggacgtg gggcttgcgc ttgcgcggca tccgctcagt
     7681 cccgcggccg gcgaaacccc gcgccgcgca ggaccagctc ggtccagcgt cgcaggaaaa
     7741 tggcggcgac ctcgcgcgtc aatttccaga ccgcaacgcc gcgccgcaca ggaacgcgcc
     7801 agtccgtggg aagggccaag gtttttctcc gtcagtcggc ggccgcgggc gccaacgcct
     7861 caaagaagcc gtcggagagc cggaggaccg cccgccaata aaaatcgcgc ccgccgtcca
     7921 acacggggac gagacacaaa agaaaaccgc gcgccaacag gcgcaggggg ccgcggccgc
     7981 gcagcgaaac ccccaggacc gccacgaatc cccgacggta aatgttcccg aaaacccaat
     8041 cggccgaccg caggagtttc gcaaaaacga ggaatcccaa actcccgatt ctcacccggc
     8101 cgcccgccca cgggaaacgt ctttcccgcc gccggatctc cgtgacccgc cccaccatga
     8161 tgccgtcggc ggagtcgccg gaaaaattcc aatcccccat cgtccgatag cgccacgacg
     8221 ggcccctccg gcttttccac aacagccggt ggacgacgta gcccaccgcg ggaccgccgg
     8281 ccagcgccgc caggaagacg aggtcccccg tccgcaggac ctccccctcg caacgctgaa
     8341 aacggatggc
//
LOCUS       DAOTFD010000102         8336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6049_length_8336_cov_4.129815, whole genome shotgun sequence.
ACCESSION   DAOTFD010000102 DAOTFD010000000
VERSION     DAOTFD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6049_length_8336_cov_4.129815"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="P5079_06865"
     CDS             complement(<1..2247)
                     /locus_tag="P5079_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29746.1"
                     /translation="MYATETAFWSQRRQAADKLKDKPTLDQAVLAADEPSRAGLSSEQ
                     YQLLAQLPRATDFSLGVTENVSIATLGAEPSARLKPQDLQKAAAQAKDSPTWLSTLVL
                     PFGSIRDIYLSPQPGAPLVIHIQDAHEIEEAQKNMAAMIQGLRQERGINLVGLEGAQG
                     AFAVDSFRGYADPEILKGMADYFLKEGYIGGAEYAGLTSPEPPILWGMEDTALYESNI
                     QAFKDSIKYKPVVHTFLSEMKTAVAALKEEVYSPDLKEFDKHFNAYKGHGESLGAYVR
                     YLLSVSDDGRRGYTNLHLLINALDWEDSLDFKRVEKERLELVEMLAKRLSDEQLTYLV
                     QQSLLYRMGRMGYGDYYRFLRNLCRQNDIPLDNFTQLSTYITYVLLAEQINRNELLVE
                     LSRLEEAAQNGLAGTSNQRRLAAVNRHLMVLEKLTSHNMTMSDWLYYFDKKVDILGVV
                     AETRSLASAAGLTVTVNVPDNFVNVIQPYEQFCQYATQRNNAIVENMLSKMKQEKVPT
                     SILVAGGFHSDGLTQILRQKGVSYVVVMPKITEVPKESHYLDVFAHDPLPLEKMFSGD
                     TIYLAARRLMQSDPTVIDDRLRSFQGLAALLPGILEVARKAQESGVGVEELEQVLQAA
                     GEKVGRRFKTVGALSAHIHKKTRAGFFNLASVSITNDKGNERRVTAFICSKEKMEAAK
                     PYLEKAGITVAHQAEIEIDGQKYVFALSSTAPGFAPLRAFVDIFKKLSVSVVAAVGEK
                     EDEVKKAAL"
     gene            complement(2622..3539)
                     /locus_tag="P5079_06870"
     CDS             complement(2622..3539)
                     /locus_tag="P5079_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY29747.1"
                     /translation="MKIFRLWIVLCLAPSALFAAAEVEALFRRDAKAYDPQYAEMTVV
                     FLDVGQGDSTFIKTPNGRTVLIDGGGTPAWSDLDFEPGREVVVPFLEERGVLKLDYVV
                     ISHPHGDHIAGLLEVFRRVPVKNLVDPALEAEEPEYSELLKLAKKNKTEYVVVGEGDL
                     LPIDPALEVRVLSPPRDYVYPDLNNSSCVLRITYRNVSFLLTGDIEEDAENRLAREYK
                     NKLRSQILKVPHHGSRTSTSLRFLDLVKPETGIVMVGRGNLFGHPHPQSLSRFRARNI
                     LLYRTDNDGNVTVRTDGLHYKVTTAREAQ"
     gene            complement(3548..4432)
                     /locus_tag="P5079_06875"
     CDS             complement(3548..4432)
                     /locus_tag="P5079_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY29748.1"
                     /translation="MSGIRRRMRARHLLPLLILAATVVPSAWARQELVVTYLDCGQGD
                     SILLQTPDNKSILIDAGPLEASEGLDAGRDAIAPYLREQGIRRLDVVVFSHPHMDHIG
                     GFKYILKNFSVGYVYDVGYSYPSPDYKDVLSLIQKKNIKYSVARSGDTLDWGPSLKVR
                     VLGPPRELPWDNPNDNSMLIQVQHGKVKFLFTGDIEKEAEFEAVIRHGPTLESQIFKA
                     PHHAANTSSTKEFLDHVRPETVVVSCGRRNRFGHPNKKIIGRYEDYGLRVYRTDLQGH
                     IRVISDGKKYRVEAKALP"
     gene            complement(4432..5376)
                     /locus_tag="P5079_06880"
     CDS             complement(4432..5376)
                     /locus_tag="P5079_06880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29749.1"
                     /translation="MSEKFKVKSSKGLLLLALNLTLSTFNCFATEGAGTVAVPFLRMA
                     VGPRAAGMGEAFTAVSDDSTALFWNPAGLGGVRRNEVYASHQTWLEDARLSHAAFARP
                     VGRLGTFGVGLAYVGSGVLEGRNESDVVTGNFDMTDLAVTLGAGRPFFNFLQAGVNVK
                     MIQQTIADEKGSGFAADAGALWRIPLPEQVVVWGVSVNNVGGKIGPGEKADLPTAAHV
                     GVCDRLLRETVLLSVEGVLPQDTDFKVNAGAEFLVGGFLALRGGYRFRQDDVSGVDGL
                     SAGFGLRYSQKQDYFFDYSFSGQGDLGASHRVSLTLRF"
     gene            complement(5373..6611)
                     /locus_tag="P5079_06885"
     CDS             complement(5373..6611)
                     /locus_tag="P5079_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRY29750.1"
                     /translation="MAALLAALLAVGISSLRAGDLKVTYVDVGVGDAIHIQTPAGKNY
                     LIDGGDTSDPNTPPTLDDTTQDFCPLVKYLLDNNIRTLDFILKTHDDSDHYRGLQALL
                     GTKTTSGGSNVFSVGAVVFSSDTAVLTNLSSHPETKSVRVSSGTRLETLPDLAGIWDP
                     ALTGLVLYPRSPTSDPSTNSGSVVVKFTYDRLSFLFTGDAETADETAIRADFSSATLA
                     STVLKVAHHGSRTGSSSAFLDAVTPQVAVVSVAANSYGLPDAEILKRLTDKGAAVYRT
                     DQQGHVSVVTNGSAFAVYTAYVSSQTVTDLRTVSSRPSVHVYPNPAPGKTSPAKTTVV
                     YELNGVVDEVRVAVYSFNGELVRSFDGVTRHIGSNYFDWDLKNGEGEAVVNGLYYVQV
                     EAKAGGSSLFGRTKMAVLRP"
     gene            complement(6702..6962)
                     /locus_tag="P5079_06890"
     CDS             complement(6702..6962)
                     /locus_tag="P5079_06890"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HRY29751.1"
                     /translation="MAKSAKKSGGTTVMHVTVDRIEDKRTAVVTVLGGGQMFLPVKLF
                     SPGLREGQIFDVTWAPNREAEQTLRSEIMKLQGALLKRSRKR"
     gene            complement(6955..8016)
                     /locus_tag="P5079_06895"
     CDS             complement(6955..8016)
                     /locus_tag="P5079_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRY29752.1"
                     /translation="MTSKPLHAIRERIEATEAQHLAPQAQLAIRTRGRQKPDEECPVR
                     TCYQKDRDRILHSTAFRNLKFKTNVFLSTSGEKFRTRLTHVLEVSQLGRTICRALGLN
                     EDLAEATALGHDLGHTPFGHVGEHVLHRLTGGKFYHSQQSLRVVDYLEKNGKGLNLTH
                     EVRDGILKHAKGSKTMRNFDGTGAFGKPVTLEGQVIQYADWLAYVNHDVDDAVAMGVV
                     KINDFPQDALRLLGLRHSERVGSMVRAVIEASRDLKQVRLSPAVLEATEAVRDYLYKK
                     VYTRPEIADVLKQSEIVVAALYEHYWKNFDEILREWPWIEPEGKEKAVADFVAWQSDY
                     ACLERYEKIRGKGPVTSHG"
     gene            complement(8016..>8336)
                     /locus_tag="P5079_06900"
     CDS             complement(8016..>8336)
                     /locus_tag="P5079_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29753.1"
                     /translation="LEGVLVAVPEPLSPVEADKIKRTYERPALKQIVAGSEAGVQVGL
                     ADENGDTLALGVIQKFDFSERHIEIWTPLALGREDKVKILQFGTLRLTLDGREDGSVD
                     AGKF"
BASE COUNT         1584 a         2542 c         2513 g         1697 t
ORIGIN      
        1 caacgcggcc ttttttacct catcctcctt ctcccccaca gcggcgacga ccgagacgct
       61 gagcttttta aagatgtcca cgaacgcccg caggggggcg aagccgggag ccgtggaaga
      121 cagggcgaaa acgtatttct gaccgtcgat ctcgatttcg gcctggtgcg ccaccgtgat
      181 gccggctttt tccaggtaag gtttggcggc ctccattttt tctttggagc agatgaaggc
      241 ggtgacgcgt cgttcgttgc ccttgtcgtt ggtgatggac acgctcgcca ggttgaagaa
      301 tccggcgcgg gtcttcttgt ggatgtgggc gctcaacgcc cccacggttt tgaatcgccg
      361 gccgaccttc tcccccgccg cctgcaggac ctgttccagt tcctccaccc caacgccgct
      421 ctcctgggcc ttccgcgcca cttccagtat ccccggcaag agggcggcca atccctggaa
      481 cgaccggagg cggtcgtcga tgacggtcgg atcgctctgc atcaatcggc gcgcggccag
      541 gtagatggtg tcgccggaga acatcttctc caacggcagg ggatcgtggg cgaacacgtc
      601 cagatagtgg ctttccttcg gcacctcggt gatcttgggc atcaccacca cgtaggagac
      661 tcccttctgc cgaaggatct gcgtgaggcc gtcggaatgg aaaccgccgg ccaccaggat
      721 ggaagtgggg accttttcct gtttcatctt cgacagcatg ttctccacga tggcgttgtt
      781 tcgctgggtg gcgtattgac agaactgttc gtacggttgg atgacgttga cgaaattgtc
      841 cggaacgttc acggtgaccg tcagtccggc agccgacgcg agggagcggg tctccgcgac
      901 cacgccgagg atgtccacct ttttgtcgaa gtaatacagc cagtccgaca tggtcatgtt
      961 gtgcgaagtc agtttttcca acaccatcag gtggcggttc accgccgcca ggcgtctctg
     1021 gttcgaggtg ccggccaggc cgttctgcgc ggcctcctcc aagcgggaca gttccaccaa
     1081 aagttcgttg cggttgatct gttccgccaa gagcacgtag gtaatgtagg tgctcaactg
     1141 ggtgaagtta tcgagaggga tgtcgttctg ccggcagagg ttccggagga agcggtagta
     1201 atcgccgtag cccatgcggc ccatgcggta aagcaggctc tgttgcacca aataggtcag
     1261 ttgttcgtcg gaaaggcgct tggccagcat ctccaccagt tccaggcgct ctttttccac
     1321 ccgtttaaaa tcgagggagt cctcccaatc cagcgcgttg atgaggaggt gaaggttggt
     1381 gtagccccgc cgaccgtcgt ccgacaccga caacagatac cgcacgtagg cgcccaggct
     1441 ttccccatgc cctttgtagg cgttgaagtg tttgtcgaac tccttcaggt ccggagaata
     1501 cacctcctcc ttgagggcgg ccaccgcggt cttcatctcc gaaaggaacg tgtgcaccac
     1561 cggcttgtat ttgatggaat ccttgaaggc ctggatgttg ctctcgtaga gcgcggtgtc
     1621 ttccatgccc caaaggatgg gcggttcggg cgaggtgagg ccggcgtatt ccgcgccgcc
     1681 gatgtagccc tccttcagga aatagtcggc cattcccttg aggatctccg gatcggcgta
     1741 gccccggaac gaatccacgg cgaaggcccc ttgggctcct tccaaaccga caaggttgat
     1801 gccccgttcc tggcggaggc cctggatcat ggcggccatg ttcttctgcg cctcttcgat
     1861 ctcgtgcgcg tcctgaatgt ggatgacgag cggcgcgccc ggctggggag agaggtagat
     1921 gtctcgaatg gaaccaaacg gcaaaacgag ggtggacagc caggtggggg aatctttggc
     1981 ctgcgccgcc gctttttgga ggtcttgcgg cttcaggcgg gccgacggct ccgcgccgag
     2041 ggtggcgatg gaaacgtttt cggtgacgcc caaactgaaa tcggtggcgc gggggagttg
     2101 ggccaacaat tggtattgtt cggaggaaag accggcacgg gacggctcat ccgccgccaa
     2161 gaccgcctgg tccagggtgg gtttgtcttt cagtttatcg gccgcctgac ggcgctggga
     2221 ccagaacgcc gtctcggtgg catacaccgt taaagcgctt gaatagaaat agacaaaaga
     2281 aaaagcgacg caaaataccg acttccaacc ccttctcaca cgggtcagga accggcgttt
     2341 cggtcgccct aaaatcgctt gtggtgtacc cgaacaaacc aacgccgtct cgcggcgttt
     2401 atctgtccgg atttcctctc cagacatttc ggtttgtctc ccctctccca aggaaattac
     2461 accgatctat ggcacctctg ttactacttc tttacaccta tagtatcaat gatatattcc
     2521 tttaagtcaa gggctttttt attatttttt tatggatttt ccccctcgtc aaagggggtg
     2581 gaaaacgatc aaaaaacggc aaattttagg gttttgggcc tttattgggc ttcccgggcc
     2641 gttgtgactt tatagtggag gccatcggtg cgcacggtca cgtttccgtc gttgtccgtc
     2701 cgatacagga gaatgttgcg ggcgcgaaaa cgggacagag attgcgggtg gggatggccg
     2761 aaaaggttcc ccctccccac catgacgatg cccgtctccg gtttcaccag atccagaaaa
     2821 cgcagggagg tggaggttcg ggacccgtgg tgagggacct tcaagatttg cgacctgagt
     2881 ttgttcttgt attcccttgc caggcggttc tccgcgtcct cttcaatgtc ccctgtaaga
     2941 aggaacgaca cgttccggta ggtgattcgt aacacacagg agctgttgtt caaatccgga
     3001 tacacgtagt cccgcggcgg cgagagcacc cggacttcca acgcggggtc gatgggcaac
     3061 aggtctccct cgccgacgac cacgtattcc gttttgtttt tcttggccaa cttcaaaagc
     3121 tcgctgtatt ccggctcctc cgcttccaac gccggatcca ccagattctt caccggcacc
     3181 cggcggaaca cctccagcag gccggcgata tggtcgccgt gcgggtggga gatgaccacg
     3241 taatcgagct tcaacacccc gcgctcctcc aggaacggca ccaccacctc ccggccgggt
     3301 tcgaagtcca ggtcggacca ggccggcgtt ccgccgccat cgatgagcac ggtgcggccg
     3361 ttgggcgtct tgatgaaggt ggaatccccc tggccgacgt ccaaaaaaac cacggtcatc
     3421 tcggcatatt gggggtcgta ggctttggcg tcccggcgga aaagggcttc gacctccgcc
     3481 gcggcgaaaa gagcggaggg ggccaaacac aggacaatcc agaggcggaa tatcttcatg
     3541 agctctctca aggcaacgcc ttggcctcca cccggtattt tttaccgtcg gaaatgacgc
     3601 ggatgtgccc ctggagatcg gtgcggtaga cgcgcaggcc gtaatcctca taccggccga
     3661 tgatcttctt gttgggatgc ccgaagcggt tccggcggcc gcaggaaacg accaccgtct
     3721 ccggtcgcac atggtccaag aactccttgg tggaggaggt gttcgcggcg tggtggggcg
     3781 ccttgaatat ttggctttcc agggtcgggc cgtgccggat caccgcctcg aactccgcct
     3841 ccttttcgat gtcccccgtg aaaaggaact tcactttccc gtgttgaacc tggatgagca
     3901 tggagttgtc gttggggttg tcccagggca gttcccgagg cgggcccagg accctcactt
     3961 taaggctcgg tccccaatcg agcgtgtcgc cgctgcgcgc cacgctgtat ttgatgttct
     4021 ttttctgtat cagggacagg acgtccttgt agtccggcga gggataggaa taccccacgt
     4081 cgtagacgta gccgacggag aaatttttaa ggatgtattt gaatccgccg atgtggtcca
     4141 tgtgcggatg agaaaaaacg accacgtcca gacggcggat gccctgttcg cggagataag
     4201 gagcgatggc gtcgcggccg gcgtcgagtc cttcgctggc ctccagcggc cccgcatcga
     4261 tgaggatgga tttattgtcg ggcgtttgaa gaaggatgga atcgccctgg ccgcagtcga
     4321 ggtaggtgac gaccaactcc tgcctggccc aggcggaagg aacaaccgtc gccgccagga
     4381 tcaggagtgg gagaaggtgc cgcgcccgca tgcgacgacg aatcccgctc atcagaacct
     4441 cagagtcaga ctgacgcgat gactcgcgcc gagatccccc tgcccgctaa aagaataatc
     4501 aaaaaaatag tcctgtttct gggaatagcg gagtccgaac ccggcggaca gcccgtccac
     4561 cccggagacg tcgtcctgcc ggaaccggta tccgccgcgg agggccaaaa atccgccgac
     4621 caagaactcc gcgccggcgt tgaccttgaa atccgtgtcc tgagggagaa ccccctccac
     4681 ggaaaggagg acggtctccc ggagcagccg atcgcacacg cccacatgcg cggccgtggg
     4741 gagatcggcc ttttctcccg gcccgatctt tccgcccacg ttgttgaccg acacccccca
     4801 gacgacgacc tgttccggca gagggatccg ccagagcgcg ccggcatcgg cggcgaaacc
     4861 cgaacctttc tcgtcggcga tggtctgctg gatcattttg acgttcaccc ccgcttgcaa
     4921 aaaattaaag aacggacggc cggcgccgag cgtgacggcc aagtccgtca tgtcgaaatt
     4981 gccggtgacg acgtcggatt cgttccgccc ttccagcacc cccgacccca cgtaggccaa
     5041 ccccaccccg aacgtcccca gccgcccgac cggacgggcg aaagcggcgt ggctgagacg
     5101 ggcatcctcc agccaggtct gatgcgaggc atacacctca tttcttctaa cgccgccgag
     5161 gccggccggg ttccagaaaa gcgcggtgga atcgtccgac accgcggtga acgcctcgcc
     5221 catgcccgcg gcgcgcgggc cgacggccat ccgtaaaaac ggaacggcga ccgtgccggc
     5281 cccttcggtg gcaaaacagt taaaagttga aagtgtcaag ttcaaagcaa gcaagagcag
     5341 cccctttgaa cttttaacct taaacttttc actcatggcc gcagcaccgc catcttggtc
     5401 cggccgaaaa gggaactgcc gccggccttg gcctccacct gcacgtaata aagcccgttc
     5461 accaccgctt ctccctcgcc gttcttcaga tcccaatcga aatagttgga accgatgtgg
     5521 cgcgtgacgc cgtcgaagga acggaccaat tcgccgttaa aggaatagac ggcgacgcgc
     5581 acctcgtcca ccacgccgtt cagctcgtag accaccgtgg ttttggcggg gctggtcttt
     5641 cccggcgccg gattggggta cacatgcacc gacggccgag aggagacggt cctcaaatcc
     5701 gtcaccgtct gagaagacac ataggcggtg tagaccgcga acgcgctgcc gttggtgacg
     5761 acggaaacat ggccctgctg gtccgtgcgg tagaccgcgg cacccttatc cgtcagccgt
     5821 ttgaggattt cggcgtccgg gagaccgtag ctgttggccg ccaccgaaac aacggccacc
     5881 tgcggcgtga cggcgtccag aaatgcggaa gaagaaccgg tgcgggatcc gtggtgggcc
     5941 accttcaaca ccgtggaggc cagggtggcg gaggaaaaat cggcccggat ggcggtctcg
     6001 tccgcggtct cggcatcgcc ggtaaagagg aaactgagac ggtcgtaggt gaacttcacg
     6061 accaccgaac cgctgttggt gctgggatcc gacgtgggag agcgcggata gaggaccagg
     6121 cccgtcaggg cggggtccca gattccggcc aagtccggca gggtctccag ccgggtgccg
     6181 gaggaaaccc gcacggactt cgtctccgga tgcgaggaca gattggtcag gacggcggtg
     6241 tccgacgaaa agacgaccgc cccgacgctg aagacgttcg atccgcccga ggtcgttttc
     6301 gtgcccagaa gggcctggag gccccggtag tggtcgctgt cgtcgtgggt cttcaggatg
     6361 aagtcgagcg tgcggatgtt gttgtccaaa aggtatttga cgagggggca gaaatcctgg
     6421 gtggtgtcgt cgagggtggg gggggtgttg gggtcggagg tgtcgccgcc gtcgatgagg
     6481 tagttcttcc cggccggggt ttggatatgg atggcgtcgc cgacgccgac gtccacgtag
     6541 gtcaccttca gatcgccggc ccggagggat gaaatcccga ccgccagcaa cgccgcgagc
     6601 aacgcggcca cgcgcccaca cccccgcctc tggagtttcc ttttcctacc catcgatccc
     6661 atcatacctc attcaagcgt ttgaagaaaa gacgaaacgt cctatctctt ccgcgagcgt
     6721 ttcaggagcg ccccctgcaa cttcatgatc tccgaacgca acgtttgttc cgcctcgcgg
     6781 ttcggcgccc aggtgacatc gaagatctgt ccctcccgca ggcccggtga aaaaagtttg
     6841 acgggcagga acatctggcc gccgcccaac accgtcacca ccgcggtgcg tttatcctcg
     6901 atcctgtcca cggtgacatg catcaccgtc gtcccgccgc tttttttcgc gcttttagcc
     6961 atggctggtc acaggcccct ttccacgaat tttctcgtat cgctccaggc aggcgtaatc
     7021 cgactgccac gccacgaaat ccgccaccgc tttttccttc ccctccggct cgatccacgg
     7081 ccattctcgg aggatctcgt cgaaattttt ccagtaatgc tcgtacagcg ccgccacgac
     7141 gatctccgac tgcttgagca cgtcggcgat ctcgggacga gtgtacactt ttttatagag
     7201 atagtcccgc accgcctcgg tggcttccag caccgccggg ctcaggcgca cctgtttcag
     7261 gtcccggctg gcctcgatga ccgcccgcac catggagccg acgcgctccg aatgccgcag
     7321 accgagcaac cggagggcgt cctgcggaaa atcgttgatc ttcaccacgc ccatggccac
     7381 ggcgtcgtcc acgtcgtggt tcacgtaggc cagccagtcg gcgtactgga tgacctgccc
     7441 ctccagggtc acgggcttgc cgaaggcgcc ggtgccgtcg aagttcctca tcgtcttgga
     7501 ccctttggcg tgtttcagga tgccgtcgcg cacctcgtgg gtcaggttga gtcctttgcc
     7561 gtttttctcc agatagtcca ccacccgaag agactgctga gaatgataga acttgccccc
     7621 ggtgagcctg tgcaggacgt gttctccgac gtgcccgaag ggcgtgtgcc ccagatcgtg
     7681 ccccagcgcg gtggcctcgg ccaggtcctc gttgagcccc agcgcccgac agatggtccg
     7741 gccgagctgg gagacctcca acacgtgcgt gaggcgcgtg cggaacttct cccccgaggt
     7801 ggacaaaaaa acgttggtct tgaatttcag gttgcggaag gcggtggaat ggaggatccg
     7861 gtcccggtct ttctggtagc aggtgcgcac ggggcattcc tcgtcgggtt tttgccggcc
     7921 gcgcgtccga atggccagct gcgcctgcgg cgccaaatgc tgggcctccg tggcctcgat
     7981 gcgttcacgg atggcgtgaa gcggtttcga cgtcattaga attttcccgc gtccacagag
     8041 ccgtcctctc ggccgtcgag ggtgaggcgg agggttccga actgaaggat cttcacctta
     8101 tcctcccggc ccagggccag gggggtccat atctcgatgt gcctttcgga aaaatcgaat
     8161 ttctggatga cgccgagcgc cagcgtgtct ccgttctcgt cggccagtcc cacctggaca
     8221 cccgcctccg agcccgccac aatctgcttg agggccggac gctcgtaggt ccttttgatc
     8281 ttgtccgcct caacggggga caagggttcc gggaccgcca ccaggacccc ctccag
//
LOCUS       DAOTFD010000103         8280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6099_length_8280_cov_4.644377, whole genome shotgun sequence.
ACCESSION   DAOTFD010000103 DAOTFD010000000
VERSION     DAOTFD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6099_length_8280_cov_4.644377"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5079_06905"
     CDS             complement(<1..340)
                     /locus_tag="P5079_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRY29754.1"
                     /translation="MTRESFPRMSPSPGEFRRLLLQWYERNGRHDLPWREKPTPYAVL
                     VSEFMLQQTTVRTVLPYFQRFMKEFPTVKELARAPLDRVLEVWSGLGYYARARNLHAA
                     AQKIVKDFKGH"
     gene            509..1015
                     /locus_tag="P5079_06910"
     CDS             509..1015
                     /locus_tag="P5079_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29755.1"
                     /translation="MKKTFFLIFPATGLLFLSGPRCGAEESAATAVETASPALTAPLV
                     EKPADDRLASALRDLEDGNWRRAQRKAKSVLAGNKKNSQAWLVWGRSCLSAGKYKKAT
                     KRFQKALRYDPRFAEAYFWRARAFESWGRTDEAMNEYQAAFHADPAMESARSAWKNLQ
                     GPAPSSEE"
     gene            1063..1146
                     /locus_tag="P5079_06915"
     tRNA            1063..1146
                     /locus_tag="P5079_06915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1097..1099,aa:Leu,seq:cag)
     gene            1229..1687
                     /gene="smpB"
                     /locus_tag="P5079_06920"
     CDS             1229..1687
                     /gene="smpB"
                     /locus_tag="P5079_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRY29756.1"
                     /translation="MKVIARNRRASFRYHILETFDGGLVLTGPEVKSLRAGEVSLEDG
                     FAHMENGEVFLWNVHIQPYKQGSLHVQQNATRSRKVLLHKTETKKILGKLTIKGLTLV
                     PLELYFSDSGYAKVKLGLAKGKTGPDKRQDLKKKDVQREMRREFSGKHRV"
     gene            1689..1988
                     /locus_tag="P5079_06925"
     CDS             1689..1988
                     /locus_tag="P5079_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY29757.1"
                     /translation="MNARAFAAFGHIPGIGPSMAMDLWRLGLRRADDLKNRNAETLYE
                     KLCDLDGRRLDRCVLYAFRCAVYYVSRQKHDPKLLKWWSWKEPGCNPRLPRGPKP"
     gene            2033..2122
                     /locus_tag="P5079_06930"
     tRNA            2033..2122
                     /locus_tag="P5079_06930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2067..2069,aa:Ser,seq:cga)
     gene            2340..3833
                     /locus_tag="P5079_06935"
     CDS             2340..3833
                     /locus_tag="P5079_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29758.1"
                     /translation="MRVLFVALGAENLAFSLLAPLLEKAGHTVDLAFSASLFNDQVIS
                     LPTISRLFNDDHSIHDKAREFRPDVVCFSAFSMNHRRLLKIAGSLRESTGAKTIFGGI
                     HPSAVPELVVREPAVDYVCVGEGETALPMLLERLGRGALSAPSPNILFKSPTGEILRG
                     PQLPLHEDLDSLPGFEKDIWRDYLRLDKCYMTISSRGCPIRCTFCANDLLSSLDGRKS
                     PHFRQRSAEHFLNELKTAKRKYGITFVNIEDDAFTLDAGWLRKFSDSYRREIGVPFAC
                     LSHPLFLDEERIRLLKEAGCVSVDIGVQSVDESVRKKWLNRPEKNWHIERAVQLLEEA
                     RIPYVLDHMLGIPSETPESQERAWQFYGKLNPSRINTFWLTYYPGTEIQRIALTDKYL
                     TAEEADKINRGHLGPYYGVDVKLPAPVRRLYRSYLLLLHLSPLLPRWIKRKIGAARIA
                     NLPEGALKTLDFLAQWICFHRHPTADYRRYAAQFWNNFRIFWRRKGG"
     gene            complement(3874..4722)
                     /locus_tag="P5079_06940"
     CDS             complement(3874..4722)
                     /locus_tag="P5079_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29759.1"
                     /translation="MWEAHRSCNYRCPYCFWDPHWGKQDDSRRPAGDWIGCWGRVEEK
                     YGPLKISVVGGEPLIFPECDKLFQRLTARSFVALVSNLSPSLTSIDKLLAVVTRKNFM
                     VTASFHPHFAVFDDFAAKARLLKDAGCLESILIVAYPPQLEDIQEPMRRLESMGIGVN
                     IRTFKGEYGGRDYPACYTEEERSFLRPLLNQKSVSYNLEKRTTRGRLCYAGKYYAMVD
                     SQGKVYRCGADAQNCLGNIFDPNFELLSAPARCPSEWCACRTFQYLVEEYDKLASSGG
                     GPAKIK"
     gene            complement(4830..6008)
                     /locus_tag="P5079_06945"
     CDS             complement(4830..6008)
                     /locus_tag="P5079_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HRY29760.1"
                     /translation="MKWASAVSERPRLERAVQECAASLKEQMGGASVDFVMAFVSVYF
                     MERCFDVNALVMDALPCNVFIGCSAAGIIGAGREVENRPGLSLMAASLPKVVVKPFAL
                     RDEDLPDLDDPPRRWEALVGVKAAENPHFVLLSDPFSVRLDNFLGGMDYAFPKAVKIG
                     GVVSGALAPAQSALYLNSMCQRSGLVGVSFVGDLTMDPLVAQGCRPIGRPMTVTDCQE
                     NILLELEKRPPLQVLQDLVDSLDPNDQELAKHSLFLGIIMDPRKETYGKGDFLIRNII
                     GADMKKGILAVGTRLRKGQGVQFHLRDARAAAQELQTLLSSHGPSKDKIAGALLFSCL
                     GRGEYLYGRRDHDSQMFQRTLGQKPLGGFFCNGEIGPVGASTYLHGYTSCFALLRPKW
                     "
     gene            complement(6128..7381)
                     /locus_tag="P5079_06950"
     CDS             complement(6128..7381)
                     /locus_tag="P5079_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRY29761.1"
                     /translation="MDFRQRLVSFLGLLVLLGLCWVFSNNRSKVNWRLVAWGMALQFV
                     FALIVLKTAPGMWVFARLNDAVNALLGFTREGTTFLFRSYLTGRVESPLVNFTFDVLP
                     TIVFFSSLMTVLYHVGVMQVIVKIFAVAMQRTMKTSGAETLSTSANIFVGQTEAPLVV
                     KPFVADMTMSELMAVMVGGFANTAGGVLAAYVGMLRDVFPDIAGHLIAQSVMSAPAAL
                     VCAKIIFPETETPRTTGNTKMSVEKIDANALDAAARGATEGLQLAFNVAAMLLAFIAL
                     IGMLNAGLRALGGWTGMPSLSLELLFGYAGWPLAWVMGVPTADCFIIGKLIGVKTVVN
                     EFVAYLQLADLLKNGVPLSYRSVVIASYALSGFANFSSIAIQIGGISAIAPSRRHDLA
                     KLGLRAMFVGAIATFTTATIAGLLL"
     gene            complement(7608..7982)
                     /locus_tag="P5079_06955"
     CDS             complement(7608..7982)
                     /locus_tag="P5079_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29762.1"
                     /translation="MAGKKKILIAEDERAIAEFVEALLSLNGYETAIAGNGREAVEKA
                     RSFFPDMVLMDIMMPKLSGLDATKILRDDPAFKNTVIMMLTSLTQMGDAEKAFEAGAN
                     DYLGKPFEAERLLQKVKKHLGE"
     gene            8097..>8280
                     /locus_tag="P5079_06960"
     CDS             8097..>8280
                     /locus_tag="P5079_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRY29763.1"
                     /translation="MGGTPLLSIFASLLFLAIIFFGRGVRFYTDWLWFVDVGYLNVFW
                     TEFKTRLALGGSLAAAG"
BASE COUNT         1732 a         2614 c         2411 g         1523 t
ORIGIN      
        1 catggccctt gaaatccttg acgatttttt gcgccgcggc gtgcagattc cgggcgcgcg
       61 cgtaatatcc cagccccgac cacacctcca gaacacggtc cagcggcgcc cgcgccagct
      121 ctttcaccgt tggaaattcc ttcatgaacc tctggaaata cggaaggacg gtcctcacgg
      181 tggtctgttg gagcatgaac tccgacacca acaccgcgta cggcgtcggc ttttcccgcc
      241 acggcagatc atgcctgccg ttcctttcgt accattgcaa aaggagtctc cggaactcgc
      301 ccggggacgg gctcatccgg ggaaacgatt cccgcgtcat ttttttcatg gaaaaattct
      361 accttttttc gcggcgttcc cgaacggcgg gccgattgcc tcccccgatg gcaaatggta
      421 tatttacagg ccgttgggaa aagcgcgcgg gactggggat ttttccatct cctcgacatt
      481 tcaccttcag gggacatcag gtccatacat gaaaaaaacg ttctttctca tttttccggc
      541 gacagggctc cttttccttt ccggcccccg gtgcggcgcc gaggaatcgg ccgcgacggc
      601 cgtggaaacc gcctcccccg ccttgacggc ccccctcgtc gaaaaaccgg ccgacgaccg
      661 tctcgcgtcg gccttaagag acctggagga cgggaactgg cggcgggccc aacggaaggc
      721 aaagtccgtt ctggcgggca acaaaaagaa ctcccaggcg tggctggtct ggggacgctc
      781 ctgtctctcc gccggaaaat acaaaaaagc caccaaacga tttcaaaaag ccctgagata
      841 cgatccccgt tttgccgagg cctatttctg gcgggcgcgg gcgtttgaat cctggggccg
      901 caccgacgaa gccatgaacg aatatcaggc cgctttccat gccgaccccg ccatggaatc
      961 ggcccggagc gcctggaaga acctgcaggg gccggccccg tcgtcggaag aatagggaac
     1021 gtccttttcg aactttcaaa aaaccgtttt tgtcccggcg ttgcgcgggt ggtggaactg
     1081 gcagacacgt aagcctcagg agcttatggc cgcaaggcta tgcgggttca actcccgccc
     1141 cgcgcagtgc ccggacagga caccttccga ccctctctct tcgaccacgg gcggccgttt
     1201 tttcaaggaa tgaacgaccg gaaaatccat gaaagtcatc gcgcggaaca gacgggcatc
     1261 gtttcgttat cacatcctcg aaacctttga cggcggcctg gtgctcaccg ggcccgaggt
     1321 caaatccctc cgcgccggcg aggtcagcct cgaggacggc ttcgcgcaca tggaaaacgg
     1381 cgaggtgttc ctttggaacg tgcacatcca gccctacaaa cagggctctc tgcacgtgca
     1441 acaaaacgcc acccgcagcc ggaaagtgct ccttcacaaa accgagacga agaagatcct
     1501 cggcaaactg accatcaagg ggctgaccct ggtccccctg gaactttatt tctccgactc
     1561 gggctacgcc aaggtgaaac tggggctggc caagggaaag accggcccgg acaagcggca
     1621 ggacctcaaa aaaaaggacg ttcagcggga gatgcggcgg gaattttccg ggaaacatcg
     1681 ggtctgactt gaacgccagg gcctttgcgg ctttcggaca catcccgggc atcggccctt
     1741 ccatggcgat ggacctgtgg cgactgggcc tgcggcgggc ggacgactta aaaaaccgca
     1801 acgccgagac gttgtatgaa aaactgtgcg acctcgacgg ccgacgattg gaccgttgcg
     1861 tcctttacgc gtttcgttgc gccgtctact acgtgtcccg gcaaaagcat gacccgaaac
     1921 ttttgaaatg gtggagctgg aaggagccgg gttgcaatcc ccggctcccg cgaggtccga
     1981 aaccctgaca cagttttttg gagtgccggt ccgcgcggcc ggcgcctcca ccggagggat
     2041 gggagagtgg ttgaatccgc cgcactcgaa atgcggtgta ccttaaccgg taccgtgagt
     2101 tcaaatctca ctccctccgc cattttgcct cgggcgtttt tttcgccaca accggacaag
     2161 acgccccgat cacgtccttg tccgacggaa aacgacgcgg ggccgctccg cccccattcg
     2221 cggccgaaac aaacgcgcct cccggttttt taagcggaca aacgaaaaaa gaaatgcacc
     2281 caaccacgcc gacccgcctg gacaatttca gttttcccgg cgcggcctcc ccgaattaaa
     2341 tgcgcgtcct gttcgttgcg ctcggcgccg aaaatctcgc cttcagcctc ctcgcccccc
     2401 tgttggaaaa agccggccac acggtggacc tggcgttctc ggccagcctg ttcaacgacc
     2461 aggtcatttc cctgccgacg atctcccgcc tcttcaacga cgaccattcc atccacgata
     2521 aagcgcggga attccggccg gacgtcgtct gcttttcggc gttttccatg aaccaccggc
     2581 gattactgaa aatcgccgga tccctgcggg agtcgacggg ggcgaaaacg attttcggcg
     2641 gcattcaccc ctccgcggtg ccggaacttg tcgtccgcga gccggccgtg gactatgtct
     2701 gcgtgggcga aggggagacg gcgctgccga tgctgctgga acgtttgggc cgcggagcgc
     2761 tctcggcccc ctccccaaac attctgttca agtcgccgac gggggagatc ctccgcggcc
     2821 cgcaactccc attgcatgag gacttggact cccttccggg cttcgagaag gatatttggc
     2881 gggattacct gcgtttggac aaatgctaca tgacgatctc ctcccgcggc tgtcccatcc
     2941 gatgcacctt ctgcgccaac gacttgctct cctccctcga tggaaggaaa tcccctcatt
     3001 tccgacaacg aagcgcggaa cacttcctga acgaactaaa aacggccaaa cggaaatacg
     3061 ggattacctt cgtcaatatc gaagacgacg ccttcactct ggacgcgggc tggcttcgga
     3121 aattttccga ttcctaccgc cgggagatcg gcgtcccctt cgcctgcctg tcccacccgc
     3181 tgttcctgga cgaagaaagg atacggctcc tcaaggaagc cgggtgcgta tcggtggaca
     3241 tcggcgtgca gtcggtcgac gaaagcgtcc gaaaaaaatg gctcaaccgg cccgagaaga
     3301 attggcacat cgagcgggcc gtccaacttt tggaagaggc gcgcatcccc tatgtgttgg
     3361 accacatgct gggcatcccc tcggaaaccc ccgagtcgca ggaacgcgcc tggcaattct
     3421 acgggaagct caacccgtcc cggatcaaca ccttctggct cacgtattat cccggcacgg
     3481 aaatccaaag aatcgccctg acggacaagt atctcacggc ggaggaggcg gacaaaatca
     3541 accgcggcca cctcggcccc tactacggcg tcgacgtgaa actcccggcg cccgtccgcc
     3601 gattatatcg gagctatctc ctgcttctgc acctcagccc cctgctcccg cgctggatca
     3661 aacggaagat cggggcggct cggatcgcga atttaccgga aggggcgctg aaaaccctcg
     3721 atttcctcgc ccaatggatc tgctttcatc gccatccgac cgccgactat cggcgatacg
     3781 ccgcgcaatt ttggaataat ttcaggattt tctggcgccg caaggggggc taaaacaggg
     3841 gaacgcccgg cggcgcgggc gcacccgttc aagtcacttt attttggccg gcccgccgcc
     3901 ggagctcgca agtttatcgt attcctccac gaggtattga aacgtccggc aggcgcacca
     3961 ctcactgggg cagcgcgccg gtgcggaaag cagttcgaaa ttcggatcaa aaatatttcc
     4021 caaacagttt tgggcgtccg cgccgcaccg atagaccttg ccctgcgaat ccaccatggc
     4081 atagtatttt ccggcgtagc acaaccgtcc ccgggtggtt cgtttttcga ggttatagga
     4141 aacgcttttt tgattcagca gaggccgcag gaacgaccgc tcctcctccg tataacacgc
     4201 cggatagtcc cgcccgccgt attccccttt gaacgttcgg atgttgaccc cgatccccat
     4261 cgactccaac cggcgcatgg gctcctgaat gtcctccaac tgcggggggt aggccacgat
     4321 caatatcgac tccagacaac cggcgtcctt gaggagcctc gccttcgcgg cgaaatcatc
     4381 gaaaaccgca aaatggggat ggaagctggc ggtcaccatg aaattttttc gagtgacaac
     4441 ggccaacagc ttatcgatgg acgtgaggga cggagagagg ttgctgacga gcgcgacgaa
     4501 actccgggcc gtgagccgct gaaagagttt atcgcattcc gggaaaataa gcggttcacc
     4561 gccgacgacg gatatcttca gcggcccgta tttctcttcc acccggcccc aacatccgat
     4621 ccagtcgccc gccggacggc gggaatcatc ctgctttccc caatgcggat cccagaagca
     4681 gtaagggcac cggtagttgc acgaccggtg cgcctcccac atgaaatgaa tcctgtctct
     4741 tacggcaaac ggcatatcgg cgccagacgc acgatcggct gttaaaaaac ggccggcacg
     4801 gttctacggc gcccgcccaa cggcggacac taccacttgg gacgcaggag ggcgaaacaa
     4861 ctggtatagc cgtgcaaata cgtgctggcc cccacgggac cgatctcgcc gttgcagaaa
     4921 aaaccgccca acggtttctg gcccagcgtc cgctggaaca tctgcgaatc gtgatcccgc
     4981 cgtccgtaga ggtattcccc ccgtcccagg caggaaaaaa gaagggcccc ggcgattttg
     5041 tctttcgaag ggccgtgcga agacaacaac gtctggagct cctgcgccgc cgcgcgggca
     5101 tcccttaaat ggaactgcac cccctggcct tttcgcaggc gcgtccccac ggcgaggatc
     5161 cctttcttca tgtcggcccc gatgatgttt cggatgagga aatccccctt gccgtaggtc
     5221 tctttgcgcg ggtccatgat gatgccgagg aacagcgagt gtttcgcgag ctcctgatcg
     5281 ttcggatcga gggagtccac caggtcctgc agcacctgga gcggcggccg tttctccagt
     5341 tccaggagga tattttcctg gcaatcggtg acggtcatgg gacggccgat ggggcggcaa
     5401 ccctgggcca cgagggggtc catcgtcaaa tcgccgacga acgacacccc caccagtccg
     5461 gaccgctgac acatggaatt cagatacagc gcgctctggg cgggcgccaa agccccgctg
     5521 accacgccgc cgattttgac ggctttcgga aaagcgtaat ccatgccgcc gaggaaattg
     5581 tccagacgca cagaaaacgg gtcggacaga agaacgaaat gcgggttctc ggcggccttc
     5641 acgcccacca gcgcttccca ccgtcgggga ggatcgtcca ggtccgggag gtcctcgtcc
     5701 cgcaaggcga acggtttgac aaccaccttc ggcagggagg cggccatcag gctgaggccg
     5761 ggacggttct ccacttcgcg cccggcgccg atgatgcccg ccgcggaaca accgatgaaa
     5821 acattgcagg gcagagcgtc catgacgagc gcgttgacgt cgaaacaccg ctccatgaaa
     5881 taaacggaga caaaggccat gacgaaatcc acggacgccc cccccatctg ttccttgagg
     5941 gaggccgcgc attcctgcac ggcccgttca agacgcggtc tttcggaaac ggccgaagcc
     6001 catttcatag acgcccttcc atggaaagtt ttattaacaa aatgggatga aacttcctac
     6061 aacgggaaat tcgaggcgcg gcgggcgggc cgaaaagcaa accgcccggg cgaggtccga
     6121 aacgcggcta aagcagcagg ccggcgatgg tggcggtggt gaaggtggcg atggccccca
     6181 cgaacatcgc cctcagcccc aacttggcca aatcgtgccg ccggctcggc gcgatggcgg
     6241 agatgccccc gatctgtatg gcgatggagg agaaattggc gaagccggac aaggcgtaac
     6301 tggcgatcac cacggaccgg taggacagcg gaacgccgtt cttcaggagg tccgccagct
     6361 gcaggtaggc cacgaactcg ttgacgacgg tcttcacgcc gatgagtttt ccgatgatga
     6421 aacaatcggc cgtcggcacg cccatcaccc aggccagcgg ccaccccgcg taaccgaaaa
     6481 gcagttccag cgacagggac ggcatcccgg tccagccgcc caaggcgcgc aggccggcgt
     6541 tcaacatgcc gatgagcgcg atgaaagcca gcagcatcgc ggccacgttg aaggccagtt
     6601 ggagcccctc ggtggcgccc cgcgcggcgg cgtccagcgc gttggcgtct atcttctcca
     6661 cgctcatctt cgtgttcccc gtcgttcgcg gcgtctccgt ttcggggaag atgatcttcg
     6721 cgcacaccag cgccgccggg gccgacatga cggactgggc gatgaggtgg ccggcgatgt
     6781 ccggaaaaac gtcccgcagc attcccacgt acgccgccag cacgccgccc gcggtgttgg
     6841 cgaatccgcc caccatcacc gccatcagct cgctcatggt catgtccgcc acgaacggtt
     6901 tcaccaccag cggcgcctcc gtctggccga cgaagatgtt ggcggaggtc gacagggtct
     6961 cggcgccgga agtcttcatg gtcctctgca tggccacggc gaatatcttc acgatgacct
     7021 gcatgacgcc cacatgatag aggaccgtca tcagcgagga gaagaagacg atggtgggga
     7081 gcacgtcgaa cgtgaaattg accagcggcg actccacgcg gcccgtgagg taggagcgga
     7141 acaggaaggt ggtgccctcg cgcgtgaacc ccagcagggc gttcaccgcg tcgttcaggc
     7201 gggcgaagac ccacattccc ggggcggtct tcagcacgat gagcgcaaaa acgaactgca
     7261 gggccatgcc ccaggccacc agccgccagt tcaccttgga acggttattg gaaaaaaccc
     7321 agcagaggcc gagcagcacc agaagcccaa gaaaagaaac gagccgttgg cggaaatcca
     7381 gcggcgtgtg ttcggcctgg atgcggcgga cacggtcggc cacgacggcg gacggggcgg
     7441 cggtcgtcga aacggagacc tcggcggaag ccgcggaggc cgcgggaggg gaccacacat
     7501 aggcgaccgc ggctccgatc acgcccagga gaaaaaaaac gacgccggcc agcagcgttc
     7561 ttttcaaacg cggggacggc gtccggacgc ggtcagacaa caacggatca ctcgccaagg
     7621 tgttttttga ctttttgcag gagccgttcg gcctcgaaag gtttgcccag gtaatcgttg
     7681 gcccccgcct cgaacgcctt ctcggcgtcc cccatctggg tgagcgaggt cagcatcatg
     7741 atgacggtgt tcttaaaagc cggatcgtcg cggaggatct tggtggcgtc cagaccgctc
     7801 agtttcggca tcatgatgtc catgaggacc atgtccggaa agaaagaccg ggctttctcc
     7861 acggcctccc ggccgttccc ggcgatggcg gtctcataac cgttcaggct gagcagcgcc
     7921 tcgacgaatt ccgcgatggc gcgctcgtcc tcggcgatca gaatcttctt ttttccggcc
     7981 atgggcacat cttaccacct tgacggcgaa aaagtaaaga tgtttacggc ctctcaaaat
     8041 tcacgtatga tggtaccctg gcgacgccgg ttcctatttc gccgaggagg tcagccgtgg
     8101 gcggaacccc gttattgagc attttcgcct ctttgctttt cctggccatc atttttttcg
     8161 gccggggagt gcggttctac acggactggc tctggttcgt cgacgtcgga tacctgaacg
     8221 ttttctggac ggagttcaag acgcgcctgg cgctcggcgg ctccctggcc gcggcgggtg
//
LOCUS       DAOTFD010000104         8260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6117_length_8260_cov_3.725411, whole genome shotgun sequence.
ACCESSION   DAOTFD010000104 DAOTFD010000000
VERSION     DAOTFD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6117_length_8260_cov_3.725411"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1291
                     /locus_tag="P5079_06965"
     CDS             <1..>1291
                     /locus_tag="P5079_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29764.1"
                     /translation="PAEIKKRNSKNPNSAINRGLWTPMMMDVPGGVHRGAGFKFNDGR
                     IWQAVTDGLMIPMDAQYDGLLSDEMRQKRDEVRKMLLAEGIVSEAELDAVMLVPLHNP
                     SADPSQMALTVSDDREQVLHNRTLSFTGSDGTVYRLKGGGAASHLEEGNVELASNESN
                     GKRYAFNQGTLGGSHNFWGGIGYDAARKSAEMENELVEGLGQLATRPDVQSAIDLFPW
                     LSDIYSNPIVSFRPLMVPAVQTANAVPESGYDVSPAGVETLRKLNAGLVRGGNLQNVV
                     WIKNPAGKLKEMYPNIADLIERQRFFLYSSAEGRTLEMDARLNSSADYRTQILLSLFR
                     RIGIEVQGNSLVKDGRSLTKEDLSNEVLRAALVGFSLHLANLQSQELGATGGPAGRPS
                     DTRNSFAGTFFEDVDTLREMRRTNGFTPGDNENVKDYL"
     assembly_gap    1292..1301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1302..>8260
                     /locus_tag="P5079_06970"
     CDS             <1302..>8260
                     /locus_tag="P5079_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRY29765.1"
                     /translation="MTESYSSVHNHLYGLAEMFKAKGRFAALLRDQQDLLQWEFVCET
                     LLWFAHVNYVDGEIIDAYLDPLVGKDNEKIGNLLEILPDMSTWQQGRALFLMHMAMKR
                     GRLTEKQGRDLTDWIGRNWEADNKKTLRRMMVLTLGGIASTSYVAQLRGRVSEILESI
                     QREPVGEESWEEQLSGDVAETLLMFPGTRGFDKAFAALSAQEREKILQAYQGVEVESE
                     FDKIMAQMAPGYVRRIKNLDPQQRAAELALVRGEAVSAVPTEGLAAMLSRLDAHLGGY
                     SRDELRNVRSVKELSPEVYGALSAFYRDVTGEQGPVREAFESYLKRTSISDMLSDRVL
                     NAVKLALTEAPVGKTETVWANVNQLQKHVDAQNLSGRQMAGALSAGLQKMFGEAVNEA
                     VTQVKEIFRKKGHDPETFQFAVFHSGSVARGEGVKGSDVDSWVVYDEDGSDASVEFAN
                     ALAEEITQRMAKRGVRASPILRGFRRGAALADEFEGVSEISELMDMTVNTAHPEMLQT
                     LKAIRKQISEAEDVTEKFLRQLKEALARPLPTVAGQEKGLMRNIQIFLWWKRLQQGVP
                     DALRFGDVIRELVAKRDITESQGQRLAEAFDRFLDRRNGRPVDDAPLAVAYARAMDVI
                     KSNIASQAAGERAPSATSEEIEVVSLADAILDKLDEIMPAAAGVTVRPADSALTLPLL
                     RRLERGPAWLTFFTVPLIRLLLAPVVEAVFTLPIAALSLLGFSRTADRLTARFLGLHG
                     DMTEFQKQKRQMGIDWITRGTAVGAILGAGFAVAGLVFGMPLPEFFQVLTSAGVGMRV
                     LAATLLGGLIGNIFGGHALYNAVDLLKGWGASLTVGRTVSGRGVPVEEELLAGKEKYG
                     DVLDALVRVLDVSIAGAKDEATAETIRTLANALSEWKNPYTPAAEVEKLGIKIRETLP
                     KLLFSDGLPSRVRQVFQEAKSPEELGTKLEVVLVELLRPTQVARADAFPAKTQEPLRN
                     LDEQAPETEAERLERTMGVVPVAVEVEPLSVPIWRTLEADEKGMAQEKIRILAAPEDF
                     NGGYQNFKRQNAGRPQKILLTKTLSEDMLGYAHPGPSASLADLGRLPAEPEKIRQLGI
                     RDYILASETKNGTNAVVRLGSLNGRPVAVKAYYTRPDLDALGRENEMLRRDYRGAKAA
                     ELLGVGPRVHGVYTDSNGRQYLVTDVVAGDFLLNMQAAVSPATIVEYVDMMRRLSDAG
                     LTPKDVQFLVSADGHIRLIDAAELFYEDGRTSPVPVHQNPDVLRTMNVMLAMLPSDGD
                     RARARGLLGEKYPLLMPTAAPQKLFLSRFLGPSLYGTVQWGPLLQGVILQHKAWAEVL
                     RSVFASLPPTPNNDVLRELFEEIVAKKEMSRETAAGQVLALLAESADPLILPLGVVEA
                     VFQVFRTAENSLAPAQELSLDQLGAGKDFKKKYADLLAALGDVSLRVVRGPPIAGVST
                     DDFVTFTEEGYTVVHENTLAQWIGGEVPADLGKLKIEKEKRQPLLEDTLTALMHEALE
                     KLIITGAQRAGLTIEQVHMVVEALGFRQNNHDDSQKHRDSYLSFEAKAERFLMIQRAM
                     LNAEEGIAWDKLLQFIDAKIAESGHGADFDRVKHLGWPEWKMVAVRSILFEMAERETG
                     DKQKAEGKGLPAPYVLPAELLAETVPEGFYENLHNRVFGSRLQVVSVSPADTAGLAAP
                     AVTAVVSFAHAAAAEVVENDRAGALYREFWRLYLRAHPQGPVAEQGKRIAEIVRSAEI
                     HEVGGAGYRFYEVRDVSGQLRIYVGGKTGVILGVSFNKAEQDAVIASVVKKVQSHLDH
                     MTGETFERIVRYEQAPQCNEMLAKDEKLGVEFQRVTAEGKKEKAAPAQRPAAEPAREA
                     PRGEAAGMMAEFTAAMSAVASGAAGEWDISRLASADVEKLERARGIISRILAALDAGD
                     ADVARLLSAPGQGRWMRTYLVEIEKALYIQLKLIFPFGAGKFVVSQAWIPGYEAGNEV
                     ILSKPMTIEDAVVAARKKYLAEKERPAAPVRPVVAVPAVPVQKPAAAPVQQKPRMSAE
                     AMVAEYNAALDVIAPRVGGQRDVSRLVTADVKDLERGWKILVAILDAVDGGDAEVLRL
                     VRAPARMQAYLKEIEKVLYVKLGVVSRWEGGQFVVVQTWKTEMREGEEMIYSAPMTLD
                     EAVAAARKVYERSVSLPASAPAVQPAPLRLADLQTFDDLFRYLEGLGGRDVNGMEVKD
                     IIQIIQGVRGGTLPAKLVTRGGQDPNGPFYGLRDKVVELMDRESPRPYNLVHNVTALV
                     VTVGAGLLAAYLGVDLPSWFEWMLGVYTGLMGLHLLAGGWVRRLGWRARLESPEAKVN
                     ARLEQLVRSYYGA"
BASE COUNT         1642 a         2171 c         2827 g         1610 t
ORIGIN      
        1 ccggccgaaa ttaaaaagag gaacagcaag aaccccaact ccgccatcaa ccgcggtctc
       61 tggacgccga tgatgatgga cgtgccgggc ggcgtgcatc gcggggccgg tttcaagttt
      121 aatgacggac gcatttggca ggccgtgacc gacggtctca tgattcccat ggacgcccaa
      181 tacgacggcc ttctttccga cgagatgcgg caaaagaggg acgaggtccg aaaaatgctc
      241 cttgcggaag ggatcgtctc ggaggcggag ctggacgcgg tgatgctggt gccgttgcat
      301 aatccgtcgg ccgatccttc ccagatggcg ctgaccgtga gcgatgaccg cgagcaagtg
      361 ctccacaacc ggaccctgtc gttcaccgga tccgacggga ccgtctatcg gctgaaaggg
      421 ggaggggcgg cttctcattt ggaggagggg aacgtcgaac tggcctccaa cgaatccaac
      481 ggcaagcgat acgcgttcaa tcagggaacc ctcggcggtt cgcacaattt ctggggcggc
      541 atcggctacg acgccgcccg gaaaagcgcg gagatggaga atgagcttgt tgagggactt
      601 ggacaacttg ccacccggcc ggatgttcaa tcggccatcg atcttttccc gtggctgtcg
      661 gacatctatt cgaatcccat cgtgtcgttc cggccgttga tggtgccggc cgtgcagacc
      721 gccaacgccg ttccggagag cggttatgac gtttccccgg ccggagtcga gacgctgcgc
      781 aagctgaacg ccggtttggt acgagggggg aaccttcaga acgttgtttg gattaaaaac
      841 ccggcgggca aactgaagga gatgtatccc aacatcgccg acttgatcga gaggcagcgg
      901 ttcttccttt attccagcgc cgaggggcgc accttggaga tggacgcccg gctgaattcc
      961 tccgccgatt atcgcacgca gatattgttg tcgctgttcc ggcggatcgg catcgaggtt
     1021 caaggcaaca gtttggtgaa ggacggccgg tctctgacaa aagaagacct atcgaacgag
     1081 gtccttcgcg cggccctggt gggtttttcg ctgcatttgg ccaacttgca aagccaggaa
     1141 ttgggggcca ccggaggccc cgccggccgt ccttccgaca cgcggaattc gttcgccggc
     1201 acgttttttg aagatgtgga cacccttcga gaaatgcggc ggaccaatgg attcaccccc
     1261 ggggacaacg agaacgtgaa agactatctg gnnnnnnnnn naatgacgga gtcctactcc
     1321 tccgtgcaca accacctcta cgggcttgcg gagatgttca aggcgaaggg gcggttcgcg
     1381 gcccttttga gggatcaaca ggacttgttg cagtgggagt tcgtctgcga gaccctcctg
     1441 tggttcgctc atgtgaacta cgtggacggc gaaatcatcg atgcgtattt ggacccgctg
     1501 gtcggcaagg acaatgaaaa aatcggcaac cttctggaga tattgccgga catgtccacg
     1561 tggcagcagg ggcgggcgtt gttcttaatg catatggcca tgaaacgagg gcgattgacg
     1621 gaaaaacaag gccgggacct gaccgattgg atcggacgca actgggaggc ggacaacaaa
     1681 aagacgttgc gccggatgat ggttctgacg ttgggcggga tcgcctccac ctcctatgtg
     1741 gcccaacttc gggggcgtgt gtccgagata ctggagtcaa tccaaagaga gcccgtcggg
     1801 gaggagtcgt gggaggaaca gttgagcggg gatgtggcgg agactcttct catgttcccc
     1861 ggcactcgcg ggtttgacaa ggctttcgcc gcgctgagcg ctcaggaacg ggaaaaaatc
     1921 cttcaagctt accagggggt ggaggttgaa agcgaattcg acaagatcat ggcgcagatg
     1981 gcgccggggt atgtgaggcg catcaaaaat ctcgatccgc aacagcgggc ggcggaactg
     2041 gcgcttgttc gcggagaagc cgtttcagcc gtcccgacgg aggggcttgc ggcgatgttg
     2101 tcccggctcg atgcgcattt gggcggatac tcccgggatg agctgcggaa tgtccgctcg
     2161 gttaaagaac tttcgccgga agtctacgga gcgttgagcg cgttttaccg cgacgtgacg
     2221 ggcgaacagg ggcccgttcg ggaggctttc gaaagttatc tcaagcgcac ctcgatatcc
     2281 gatatgttgt ccgaccgggt cttgaacgcg gtgaaactcg cgttgacgga agcccctgtc
     2341 ggaaagacgg aaaccgtctg ggctaacgtc aaccaactcc aaaaacacgt ggatgcgcag
     2401 aacctgagcg gccgacaaat ggcgggggca ttgagcgccg gcctgcagaa gatgttcggt
     2461 gaagccgtca acgaggccgt gactcaagtg aaagagatat ttcggaaaaa gggccacgac
     2521 ccagagacct tccaattcgc ggtgtttcat tccggttccg tcgctcgagg ggagggggtc
     2581 aaggggtccg acgtggattc atgggttgtt tatgacgagg acggttcgga cgcttccgtg
     2641 gaattcgcca atgctttagc ggaggagata acgcagcgga tggcgaagcg aggcgtgaga
     2701 gcttctccca tcctgcgcgg ttttcgccgg ggggctgccc tcgccgatga attcgagggt
     2761 gtcagcgaga tcagcgagct tatggacatg acggtcaaca ccgctcatcc ggaaatgctc
     2821 caaacgttga aagcgatcag gaagcagatt tccgaggcgg aagatgtgac ggaaaagttt
     2881 ctccggcaat taaaggaggc cctcgcgaga cctttgccca ccgtcgctgg acaggaaaag
     2941 ggtctcatgc gcaacatcca aatcttcctc tggtggaagc ggctgcagca gggggtgccc
     3001 gatgcgcttc ggtttggcga cgtgatccgc gagttggtcg ctaagcggga tatcacggaa
     3061 agccagggcc agcggctggc ggaggcgttc gacaggtttt tggacagacg caacggacgg
     3121 ccggtggatg acgctcccct tgccgtggcg tatgcgaggg caatggacgt catcaaatcg
     3181 aacatcgcga gccaagcggc gggggaacgt gcaccttcgg cgacatcgga agagatcgaa
     3241 gtcgtgtctt tggcggatgc cattctcgat aaacttgatg aaatcatgcc tgcggcggcc
     3301 ggagtaacag ttcggccggc tgattcggca ttgaccctgc cccttcttcg ccggctggaa
     3361 cgcggcccgg cgtggctgac gttcttcact gttcccctca tccgcctctt gctggcgccc
     3421 gtggtggagg cggttttcac tcttcccatc gccgctttgt ctttgctggg tttctcccgc
     3481 accgccgacc gcctgacggc ccgcttcctc ggcctgcacg gcgacatgac cgaatttcag
     3541 aaacagaaac ggcagatggg catcgactgg atcactcgcg ggacagcggt gggggcgatc
     3601 ctgggggcgg gttttgcggt ggcggggttg gttttcggta tgccgttgcc cgagttcttc
     3661 caggtgttga cctcggcggg cgtggggatg cgggtgttgg cggccacgct gttgggcgga
     3721 ttgatcggca atatttttgg cgggcacgct ctttataacg cggtggacct cctcaaagga
     3781 tggggtgcct ctttgaccgt tgggaggacg gtctcgggta ggggagttcc cgtggaggag
     3841 gagttgttgg cggggaaaga gaaatacggc gacgttctgg atgcgctcgt ccgggttttg
     3901 gacgtgtcga tagcgggggc gaaggatgag gctaccgcgg aaaccatccg cacgttggcc
     3961 aatgcgttga gcgaatggaa gaacccttac acgccggcgg cggaagtgga aaagctgggg
     4021 attaaaatca gggagacgct ccccaaactg ttgttctcgg acgggcttcc gtcgcgcgtt
     4081 cgacaggtgt ttcaggaagc gaagagcccg gaggaactcg ggacaaagct ggaagtcgtt
     4141 ttggtggaat tgttgaggcc cacccaagtg gcccgggcgg atgccttccc ggcaaaaacg
     4201 caggagccgt tgcggaacct tgatgaacag gcgccggaaa ccgaggccga gaggttggaa
     4261 cggacgatgg gcgtcgttcc cgtcgccgtc gaagtggagc cgctttcggt cccgatctgg
     4321 cggaccctgg aggcggatga gaagggcatg gcccaggaaa aaatccggat tctcgccgcg
     4381 ccggaagact tcaacggcgg atatcagaat ttcaagcggc aaaacgcggg tcgtccgcag
     4441 aaaattctcc tgacgaagac attgtcggaa gatatgttgg ggtatgccca cccgggcccg
     4501 tccgcatcgc tggccgatct tggtcgattg ccggcggaac cggaaaaaat ccggcagttg
     4561 ggcatccgcg attacattct ggccagcgaa accaaaaacg gcaccaacgc cgtcgtccgg
     4621 ctcggctcgc tgaacggtcg gcccgtggcc gtgaaggcct attacacccg gcccgacctg
     4681 gacgcgttgg ggcgggaaaa cgagatgttg cggcgggact accgcggcgc caaggcggcg
     4741 gagttgttgg gcgtggggcc gcgtgtgcac ggtgtgtata cggattccaa tggccggcaa
     4801 tatcttgtga ccgatgtcgt ggcgggcgat ttccttctga atatgcaagc cgccgtttcg
     4861 ccggcgacca tcgtggaata tgtggacatg atgcgtcgac tctcggacgc cggtttgacg
     4921 cccaaagacg tgcagttcct ggtctcggcc gacgggcaca tccggttgat cgacgcggcg
     4981 gaactatttt acgaagacgg gcgaacgtcg cccgtgcccg tgcatcaaaa ccccgacgtg
     5041 ctccggacga tgaacgtgat gctggcgatg ttgccgagtg acggcgatcg cgcccgggcg
     5101 cgcggcctgc tcggcgagaa atatcccttg ttgatgccga ccgcggcccc gcaaaagctt
     5161 ttcttgtcgc ggtttctcgg cccgtcgttg tatggcaccg ttcagtgggg cccccttctg
     5221 caaggggtca tcctgcagca taaggcctgg gcggaggtgc tgcgctcggt gttcgcctcg
     5281 cttcctccca cgccgaacaa cgatgtcctg cgggaattgt tcgaggagat cgtcgccaaa
     5341 aaggaaatga gccgcgaaac cgccgccggg caggttttgg cgttgctggc ggaatccgcc
     5401 gacccgctca ttctgcccct cggcgtcgtt gaggccgttt ttcaggtgtt ccggacggcg
     5461 gaaaacagtc tggcgcccgc gcaggaattg tcgttggacc agctgggggc cggcaaagat
     5521 ttcaagaaaa agtacgcgga tttgttggcc gcgctgggcg acgtgtcatt gcgcgtggtg
     5581 cgcgggccgc ccattgcggg cgtgtccacc gacgatttcg tcacgttcac ggaagaaggt
     5641 tacaccgtcg tccatgagaa caccctcgcc caatggatcg gcggcgaagt ccccgccgac
     5701 ctcggcaagt tgaaaatcga aaaagagaag agacagcccc tcctcgagga taccctgacc
     5761 gcgctgatgc atgaggcatt ggaaaaactc atcatcaccg gcgcccagcg tgccggattg
     5821 accatcgaac aagtgcatat ggtggtggag gcgctcgggt tccggcagaa caaccatgac
     5881 gattcacaga agcatcggga cagctacctc agtttcgagg cgaaagcgga gcggttcctt
     5941 atgattcaac gggcgatgtt gaatgcggag gaggggatcg cctgggataa actgttgcag
     6001 ttcatcgatg ccaaaatcgc ggagtccgga cacggcgcgg attttgaccg ggtcaaacat
     6061 ctgggttggc ccgagtggaa gatggtcgcg gtgcgatcca tcctttttga aatggcggag
     6121 agggagacgg gagataaaca gaaagcggaa gggaaggggc ttccggctcc ttacgtcctg
     6181 ccggccgaat tgcttgccga aactgttccg gaaggttttt acgaaaacct gcacaatcgt
     6241 gtgttcggca gccggctgca ggtggtgtcg gtatcgccgg cggatacggc cggtttggcg
     6301 gcgccggccg tcacggcggt ggtgtccttt gcccacgcgg ctgcggcgga agtggttgaa
     6361 aatgaccggg ccggggcgtt gtatcgggaa ttctggcggc tctatctgcg ggcgcatccc
     6421 cagggccccg tggccgagca gggcaaacgg atcgcggaaa tcgttcgcag tgcggagatc
     6481 cacgaggtgg gcggggccgg gtatcgtttc tatgaggttc gggacgtcag cggtcagttg
     6541 aggatttatg tggggggaaa gacgggtgtc atcctggggg tgtccttcaa taaggcggaa
     6601 caagacgccg tcatcgcttc ggttgtcaaa aaagtccagt cccatctcga ccacatgacg
     6661 ggggagacgt tcgagcggat cgtgcggtat gaacaggccc ctcagtgcaa tgagatgctg
     6721 gccaaggacg agaaattggg tgttgaattt cagcgggtga ccgcggaggg aaagaaagag
     6781 aaggcggctc ccgcccaaag acccgccgcc gaaccggcgc gggaagcgcc tcggggggag
     6841 gcggcgggga tgatggccga attcaccgcg gccatgtcgg cggtggcttc gggcgcggcg
     6901 ggcgaatggg atatttcccg tctggcgtcg gccgacgtcg agaagttgga gcgcgcccgg
     6961 ggcatcatct cgaggatatt ggcggctctt gacgcggggg atgccgacgt ggcccgcctc
     7021 ttgagcgcgc cgggccaggg ccggtggatg cggacgtatt tggtcgagat cgagaaagct
     7081 ctctacatcc agttgaaact tatatttcct ttcggagcag ggaagttcgt tgtgtcccag
     7141 gcgtggattc cgggttatga ggccgggaac gaggtgatcc tctcgaaacc catgacgatt
     7201 gaagacgccg tggtcgcggc ccgaaagaaa tatctggcgg agaaagagag gccggcggcg
     7261 ccggtccggc cggtggttgc cgtcccggcg gtgccggttc agaagcccgc cgccgcgccg
     7321 gttcaacaga aaccgcggat gagcgccgag gcgatggtcg ccgaatataa cgcggcgttg
     7381 gatgtgatcg cgcctcgcgt gggtggccag cgggacgttt cccgtcttgt gacggcggac
     7441 gtgaaggatc tggaacgcgg atggaaaatc ctggtcgcga tcttggacgc cgtcgatggc
     7501 ggagatgccg aggtgctccg cctggtgagg gcgccggcgc ggatgcaggc gtatttgaag
     7561 gagattgaga aggtccttta cgtcaaattg ggcgtcgtct cccggtggga ggggggacag
     7621 ttcgtcgttg tgcaaacctg gaagacggag atgagagaag gggaggagat gatatactcg
     7681 gcgccgatga cgttggacga ggccgtggcg gcggcgagaa aggtctatga acggagtgtg
     7741 tctcttccgg cgtcggcccc cgcggttcag ccggcaccgc tgagattggc ggaccttcag
     7801 accttcgatg atttgttccg gtatttggaa gggctcggtg gccgggacgt caacgggatg
     7861 gaggtcaaag acatcatcca gattatccaa ggggtccggg gggggacgtt gcccgcgaaa
     7921 ctggtgaccc gaggcgggca ggaccccaac ggtcctttct atggattgag ggacaaggtg
     7981 gtcgaattga tggatcgcga gtcgccccgg ccctacaacc tggtgcacaa cgtgacggcg
     8041 ctggtggtga ccgtcggagc gggactcctg gcggcttatc ttggtgtgga tcttccttct
     8101 tggttcgagt ggatgctggg cgtgtatacg gggttgatgg gactgcatct tttggccgga
     8161 ggttgggtgc gtcgcttggg ctggcgcgcg cggctggaaa gccccgaggc caaggtgaat
     8221 gcccggttgg aacaactggt gcgttcctat tacggcgcca
//
LOCUS       DAOTFD010000105         8246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6131_length_8246_cov_4.758515, whole genome shotgun sequence.
ACCESSION   DAOTFD010000105 DAOTFD010000000
VERSION     DAOTFD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_8246_cov_4.758515"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="P5079_06975"
     CDS             complement(<1..1839)
                     /locus_tag="P5079_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY29766.1"
                     /translation="MVLHSMKCVHNREDLCAAPKAPRAGRRRSRWRAAAGGALLAAVA
                     VLPVSAYIDPGSAGLPFQTLGPFLGMLAALFAAGLVFLRTTWGRFRKPFYRLLLLLAC
                     LLLLSGLIFHILKPRMTPPSKVIVIGMDGLDHRLLKTWMEDGTLPHFSAVAGKGVFLP
                     LETVSPPQSPVAWCTFATGANPGVHGVFDFLQRTPKTYYPHLTLLKTAVNSSPWNTVP
                     FEKVRAAPAFWELTTAKKIPTVVVRHPVTFPPEKVEGRMLSGLGVPDLLGGLGRYTFY
                     TVPPFEKPADFRGELVFLKSAGALWTGDIPGPKNPSSQKSVSVPFTLSPRAAPQKSLR
                     LTVEKKSYDVEESGWSPWVRVSFRPGTLKKIHGLCRFYLASLSPLRLYQTPLNFDPEE
                     PAFPISYPSNYSKKLAESIGPYYTLGMDEDTNALSDEVISDEAFLEQCGQIMDQRDAM
                     LREELKRFESGLLMCVYDTPDRIQHMFWRAVDETHPLHGRSAAQREAVRGIYRRLDET
                     LGHVLPFVDDKTLLVVLSDHGFASFRRAVHLNRWLVDNGYLVLKDPRTAPEHFFTGVD
                     WAKSKAYSLGLGGGIYINLQGREPAGIVAPAEADKLAEELMAKIQNL"
     gene            1878..2963
                     /gene="hemE"
                     /locus_tag="P5079_06980"
     CDS             1878..2963
                     /gene="hemE"
                     /locus_tag="P5079_06980"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRY29767.1"
                     /translation="MSFPKKPDERILKESLFLKACRRERTDRAPLWLMRQAGRFMKFY
                     RELRAKVSLRELCRRPDLSAEVTVEAVRRLGVDAAIIFSDLLLPVEPLGFRLEYTAGE
                     GPRIHPPLRRASDLARLKNVDVEESLGYVAAAVRRARAALPSDVPLIGFSGAPFTLAA
                     YLIEGGGSRDFVRTKSFMHEEPRAWHRLLRRLSRTAADLLNLQIRAGAQAVQVFDTWA
                     GCLSPDHYRRFVLPHSRALFSRLDSSVPVIHFATNAGGFLEALLEAMPRENVRRPVIG
                     LDWRVDIAQARRRLGRAAVMGNLDPAVLLSPRANIRREAEKVFASAGARPGYIFNLGH
                     GVLPQTPEENVRYLVDVVKEISSGKRK"
     gene            2968..3912
                     /gene="hemH"
                     /locus_tag="P5079_06985"
     CDS             2968..3912
                     /gene="hemH"
                     /locus_tag="P5079_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRY29768.1"
                     /translation="MTDKERSSVDGRAVLVLAYGGPEKKEDIGPFLDNVLQGRPLPPE
                     RRRELAGYYERLGGVSPVTVLTPHLARELGETFRREGTETAVYVAMRHGKPSIPEVVR
                     RMAEAGVRRAVGVVLSVFRSEFSWDGYLAAVDEAVRALGPAAPTVRYVEPWHEEALFI
                     EAQADRIEEVKKDWPADRLARARWIFTAHGIPCAADPSSGYSQQFARSAELLARRLGV
                     RDYVLAYQSRGGRPQDPWLGPDLQDVLNAPSDSKDVLVVPLGFLSDHMEVLYDLDVKA
                     QRVAAAAGLRFARAKTVGTHPAFLRLLAGRIGQTWGKF"
     gene            3916..5379
                     /gene="hemG"
                     /locus_tag="P5079_06990"
     CDS             3916..5379
                     /gene="hemG"
                     /locus_tag="P5079_06990"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRY29769.1"
                     /translation="MTSPRVLVVGAGVGGLSAAVHLDELARSRNFPVELRLLEAAPRA
                     GGVVSTERRDGFLLEHGPDNFITEKPWGLDLCRRLGLEPEIIGTRREFARSFVVWGGK
                     LIPTPEGFYLLAPSRWMPMITTPLFSWRGKIRMAAELWIPRAKGRDDESLASFVRRRF
                     GGEALERMAQPMVAGIYAADPETLSLRATFPKFLQWESEHGSVLGAMLAARRRKTVVS
                     ARGPRYGIFVTLRDGLGRLTEELQRRLPAGALRLDCAVTALRRAGGRWRVATADGREE
                     TADAVCLALPAVSSAPLLHSLDPALADALRSVPYNASAVIHFAYRRSAVRHPLDGFGF
                     VVPEAENRPLSACTFSHVKYEGRAPAEHALLRVFVSGPRSGPLLEKSDAELTETVRKS
                     LEKLLGIKENPLFSRLARHAVSLPQYTLGHLDRTAVVEDRVRKWPGLALAGNAFSGVG
                     IPDCVRSGEAAARSIFEMLNGGSPAERASALPTGALG"
     gene            5376..6587
                     /locus_tag="P5079_06995"
     CDS             5376..6587
                     /locus_tag="P5079_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY29770.1"
                     /translation="MKNGPVPGERPALPELSDDQAAVLRAALDWYGKSDKQVLTIGGY
                     AGTGKTTLIGILRNELARRRKDLRVALAAYTGKASRVLESKIRDAFADFSGDYVGTLH
                     GLIYSRRLEEHGRLVGWRRRPAIDYDFIIVDEASMVDEEIWRDLLSFGKRVVAVGDHG
                     QLPPIDGKFNLMESPDLRLLRIHRQAAKNPILRLSQQIRENGVLPEQPSPPEIRFLDR
                     GSEEAGRFLEDVFAAAGKDTLVLCGSNQTRVGLNRRIRESRGFEGPSPERGDRVICLR
                     NNWEAYPRPICNGMLGTVTRMEPVLPDASVFSAVVAMDGEDAPYEGYISGRCFDQSRC
                     GESLSKRENFEGSPVDYFDFGYALTVHKAQGSEAPAVAVFVENFWSGETLKRWLYTAV
                     TRARERLIIIR"
     gene            6601..>8246
                     /gene="malQ"
                     /locus_tag="P5079_07000"
     CDS             6601..>8246
                     /gene="malQ"
                     /locus_tag="P5079_07000"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRY29771.1"
                     /translation="MRAKNNEGKDAEVYRALLETPSGKNWERLGLARRSGVVAPLFSV
                     YSADSVGIGELADLDRLADWCRATGMSLLQLLPMNDVGFDFQPYSAQSSFALEPMYLS
                     FKRLTGVRLSPFLEDIAALKGRFPTGPGRVNYGIKGAKLGLLWEIFRVSHRKGLASFE
                     KFVEQNRFWLHDYAVFKTLKEVFGQSGWESWEEPFRRKDPAALERFAQEHEQQIEFQQ
                     WLQWQLYEQFRAAKRYARKQGVFLIGDIPFLVSRDSADVWANQSYFKLHLASGAPPDM
                     FFATGQRWGMPPYDWSRIEEHRYDYVVEKLRYAEHFFDFYRIDHVVGIFRVWTIPLSE
                     PAETGGLNGVFDPKDENEWEEHGRTLLRVMIDNTRMIPCAEDLGVVPKCSNKVLEELG
                     IPGMDVQRWTRDYEKTFDFKAPEAYRSVALATVGTHDTSSLRGWWGFEAGTIDEMLFR
                     RKCASRNISYDEVRDRLFDMSRAAHGRLRWRPEVTDAETLLRIIDRREEEARDFISLY
                     KEACDEKERFLAFVGLDRPADSPGDAAFVRAALQKAGDSIA"
BASE COUNT         1394 a         2647 c         2751 g         1454 t
ORIGIN      
        1 caggttttgg attttggcca tgagttcttc cgcaagcttg tccgcctccg ccggggccac
       61 gatgcccgcc ggttcccgcc cttggaggtt gatgtagatc ccgccgccca gccccagcga
      121 atacgccttg gatttcgccc agtccacgcc ggtgaaaaaa tgctccggcg cggtccgggg
      181 atccttcagg acaagatagc cgttgtccac cagccagcgg ttcaggtgca ccgcccgtcg
      241 gaacgaggcg aaaccgtggt ccgataacac caccagcagg gtcttgtcgt ccacgaaagg
      301 caggacgtgg ccgagggtct catcgagccg gcggtagatg ccccgcaccg cttcccgctg
      361 ggccgccgaa cggccgtgga gcggatgcgt ctcgtccacc gcgcgccaga acatgtgctg
      421 gatccggtcg ggggtgtcgt aaacgcacat caacaggccc gattcgaaac gtttcagctc
      481 ctcccggagc atggcgtcgc gctgatccat gatctggccg cattgttcca ggaacgcttc
      541 gtcggaaatc acctcgtcgc tcagggcgtt ggtgtcctcg tccatgccca gcgtgtagta
      601 agggccgatg ctttcggcca gttttttgga atagttcgac gggtagctga tggggaaggc
      661 cggttcttcc ggatcgaaat tgagcggcgt ctggtagagc cggagggggg agagggacgc
      721 cagatagaaa cggcagaggc cgtggatttt tttcagcgtg cccggccgga aggagacccg
      781 gacccacggg ctccagccgg attcttcgac gtcgtaggat tttttttcga cggtgaggcg
      841 caggcttttt tggggcgcgg cccgcggcga gagcgtgaag gggacggaaa cggatttttg
      901 ggaggagggg tttttcgggc ccgggatgtc gcccgtccag agggccccgg cggatttcag
      961 gaacaccagt tctccccgga aatcggcggg tttttcgaag ggggggacgg tgtagaacgt
     1021 gtaccggccc agcccgccga gcagatcggg cacgcccagg ccggagagca tgcggccctc
     1081 cactttttcc ggcgggaacg tcaccggatg ccgcaccacg accgtgggta tttttttggc
     1141 cgtggtgagc tcccagaagg ccggcgccgc ccggaccttt tcgaagggga ccgtgttcca
     1201 cggcgaactg ttcacggcgg ttttcaggag cgtcaggtgg gggtagtagg ttttcggcgt
     1261 ccgttggagg aaatcaaaca ccccatgcac gcccgggttg gctcccgtgg cgaaggtgca
     1321 ccacgccacc ggcgactgcg gcggcgaaac ggtctccagc ggcaggaaca cccccttccc
     1381 ggccaccgcc gaaaaatgcg gcagcgtccc gtcctccatc cacgttttca gcagacgatg
     1441 gtcgagtccg tccatcccga tcacaatgac tttcgaagga ggggtcatcc tgggttttag
     1501 gatatggaat attaagcccg ataacaagag gagacacgcc aggagcagga ggagccggta
     1561 aaaaggtttg cggaaccggc cccacgtcgt ccggagaaaa accagaccgg ccgcgaaaag
     1621 ggccgccagc attcccagga acgggcccag cgtttggaac ggcagcccgg ccgagccggg
     1681 gtcgatgtac gcggagaccg gaaggacggc gacggccgcc aggagggccc cgccggccgc
     1741 ggcccgccat cgggagcggc gccggccggc ccgcggcgct ttcggagcgg cgcaaaggtc
     1801 ttctcggtta tgcacgcatt tcatagaatg aaggaccata tcttagcaaa gtggaacggg
     1861 agaagtcgga cgaagaaatg agcttcccaa aaaagccaga cgaacggatc ctgaaagaga
     1921 gccttttcct gaaagcctgc cggcgggaac ggaccgaccg cgcgcctctc tggctgatgc
     1981 gccaggcggg acggttcatg aaattttacc gggaactgcg ggccaaggtc tccctgcggg
     2041 aactctgccg gcggccggac ctttcggcgg aggtgacggt ggaggccgtg cggcggttgg
     2101 gggtggacgc cgccatcatt ttttcggacc tgctcctgcc cgtggaaccg ttggggttcc
     2161 ggttggaata caccgccggc gaagggccgc ggatccatcc gccgcttcga agggcttctg
     2221 acctcgcgcg cttgaaaaac gtggacgtgg aggaatcgtt gggatacgtg gcggcggcgg
     2281 tccggcgggc ccgcgccgcg cttccgtcgg acgtcccgct catcggtttt agcggcgcgc
     2341 cgttcaccct ggccgcctat ctgatcgaag gcggggggtc gcgcgatttt gtccgcacga
     2401 aatccttcat gcacgaagag cctcgggcct ggcatcgact tctccgccgg ttgtcgcgca
     2461 cggcggccga tctgttgaac cttcaaatcc gggccggcgc tcaggcggtc caggtgttcg
     2521 atacctgggc cggctgcctc tctcccgacc attaccgccg gttcgtcttg ccgcacagcc
     2581 gggccctttt ttcccgcctc gattcgtccg tgccggtgat ccatttcgcc accaacgccg
     2641 gcggatttct ggaggcgctc ctcgaagcca tgccgcggga aaacgttcgt cgtccggtga
     2701 tcggtctcga ttggcgcgtg gatatcgccc aggcgcgccg gcgtctgggc cgcgccgccg
     2761 tgatggggaa cctcgacccg gccgtgctgc tgtctccccg cgcgaacatc cggcgggagg
     2821 cggaaaaagt ttttgcctcc gccggcgccc ggcccggata tattttcaac ctgggccacg
     2881 gcgtcctgcc gcaaacgccc gaagagaacg tgcggtatct ggtggacgtg gtgaaagaga
     2941 tttcatccgg gaagagaaaa taaaaacatg accgacaagg agaggtcgtc ggtcgacggc
     3001 cgggccgtcc tcgttttggc ctacggcggt ccggagaaaa aagaggacat cggtcctttt
     3061 ctggacaacg tccttcaggg gcgccccctc ccgccggaac gccgccggga actggccggt
     3121 tactacgagc ggctgggcgg cgtttccccc gtcaccgttt tgacgccgca cctggcgcgg
     3181 gaactggggg aaaccttccg gagggaaggg accgagacgg cggtgtacgt ggccatgcgg
     3241 catggaaaac cgtccatccc cgaggtcgtc cggcggatgg cggaggccgg ggtgcgccgc
     3301 gcggtgggag tggtcctttc cgtcttccgg tcggagttca gctgggacgg ctacctcgcc
     3361 gcggtggacg aggcggtccg ggcgctgggg cccgcggctc cgacggtccg ttacgtggaa
     3421 ccttggcatg aagaggccct gttcatcgag gctcaggcgg accggatcga agaagtgaaa
     3481 aaagactggc ccgccgaccg gctggcccgg gcgcgatgga ttttcaccgc ccacggcatc
     3541 ccgtgcgccg ccgacccgtc ttcgggatat tcacagcagt tcgcccgctc ggcggagttg
     3601 ctggctcggc gactgggcgt tcgcgactac gtcctggcgt atcagagtcg cggcggccgg
     3661 cctcaagatc cctggctcgg gcccgatctc caggacgtcc tgaacgcccc gtccgattcg
     3721 aaagacgtcc tcgtcgtccc cctcgggttt ctttccgacc acatggaggt gctctacgat
     3781 ttggacgtca aagcccaacg cgtcgccgcc gccgccgggc tccggttcgc ccgcgccaaa
     3841 accgtcggca cccaccccgc tttcctgcgt ctcctggccg ggcgcatcgg ccagacgtgg
     3901 gggaaatttt aaaacatgac ctctccccgc gtgctcgtcg tgggtgccgg cgtcggcggc
     3961 ctgtccgcgg ccgttcacct cgacgagctc gcccgctccc gaaactttcc cgtggagctc
     4021 cggctgctgg aggccgctcc ccgcgccggc ggcgtcgtct ccaccgagcg ccgcgacggt
     4081 tttcttttgg agcacggacc cgacaatttc attacggaga aaccgtgggg tctcgacctt
     4141 tgccggcggt tgggactgga accggagatc atcggcaccc ggcgggagtt cgcgcggagt
     4201 ttcgtggtgt ggggcgggaa attgatcccg acgccggaag gtttttatct gctggcgccc
     4261 tcccgatgga tgccgatgat caccacgccg cttttttcct ggcgggggaa gatccgcatg
     4321 gccgccgagc tgtggatccc gcgcgccaaa ggacgggacg acgaaagcct cgcgtccttc
     4381 gtccgccgcc ggttcggcgg agaagcgttg gagcgcatgg cccagcccat ggtggccggc
     4441 atctacgccg ccgacccgga aacgctgagc ctgcgcgcca cgttcccgaa atttttgcag
     4501 tgggagtcgg aacacggcag cgtcctcgga gccatgttgg ccgcccgccg ccggaagacc
     4561 gtcgtctcgg cccgcgggcc gcgttacggg attttcgtca ccctgcggga cgggctcggc
     4621 cgcctgacgg aagaacttca gcggcgactg cccgccggcg ccctgcgttt ggactgcgcg
     4681 gtgacggcgc tccgccgggc cggcggacgg tggcgggtcg ccaccgccga cggccgggag
     4741 gaaacggcgg acgccgtgtg tctggcgctg ccggccgttt cctcggcgcc gttgctccac
     4801 tcgttggacc cggcgctggc cgacgccctg cggtccgttc cctacaacgc ctcggcggtc
     4861 attcatttcg cctatcgccg gtcggccgtg cggcatccgc tcgacgggtt cggtttcgtg
     4921 gttccggaag cggaaaaccg gcccctgtcg gcctgtacct tcagccatgt gaaatacgag
     4981 ggccgcgccc cggccgagca cgccctcctg cgcgtttttg tgagcggccc gcggtcggga
     5041 ccgctcctgg aaaaaagcga tgcggaattg acggagaccg tccggaaatc tctggaaaaa
     5101 ctgctcggaa taaaagaaaa tcctcttttc agccgcctcg cgcggcatgc cgtctcgttg
     5161 ccgcagtata ccctcggtca tctggaccgc acggcggtcg tcgaggaccg cgtgagaaaa
     5221 tggcccggcc tcgccctggc ggggaatgcg ttttccggcg tgggaatccc ggactgcgtc
     5281 cggagcggcg aggcggccgc ccggtcgatc tttgaaatgc tgaacggcgg ttccccggcg
     5341 gaacgagcct ccgcccttcc gaccggggct ctcggatgaa gaacggaccc gtccccggcg
     5401 agagaccggc gttgccggaa ctgagcgacg accaggccgc cgtccttcgg gccgcgctgg
     5461 attggtatgg gaagtccgac aaacaggtcc tgaccatcgg cggttacgcc ggcaccggca
     5521 agaccacgct gatcggtatt ttacggaacg aactggcgcg ccggcggaaa gatctgcgcg
     5581 tggcgctggc ggcctacacg ggaaaggcca gccgcgtcct ggaatccaag atccgcgacg
     5641 ccttcgcgga tttttccggc gattatgtgg gaaccctcca cgggctcatc tacagccggc
     5701 ggctggagga acacggccgc ctcgtcggct ggcgccgccg ccccgccatc gattacgatt
     5761 tcatcatcgt ggacgaggcc tccatggtgg acgaggagat ctggcgcgac ctgctgtcgt
     5821 tcggaaagcg cgtcgtcgcg gtcggcgacc acggccagct gccgcccatc gatgggaaat
     5881 tcaacctcat ggagtcgccg gacctgcgcc tgttgcgcat ccaccggcag gcggcgaaaa
     5941 atcccatcct gcggctgtcc cagcagatcc gcgagaacgg ggtcctgccg gaacaaccgt
     6001 cgcccccgga gatccgtttc ctcgacaggg gttcggagga ggcgggccgt tttctggagg
     6061 acgtgttcgc cgccgccgga aaggacacgc tcgtgctgtg cggctccaac cagacccgcg
     6121 tgggcctgaa ccggcgcatc cgcgaatcgc ggggtttcga ggggccctcg ccggaacgcg
     6181 gcgaccgggt gatctgtctc cgcaacaact gggaggctta tccccgtccc atttgcaacg
     6241 gcatgctggg gacggtgacc cggatggagc cggtcctgcc ggatgcctcc gttttttcgg
     6301 cggtggtggc gatggacggc gaggacgccc cctacgaggg ctacatctcc gggcggtgtt
     6361 tcgaccagtc ccgttgtggc gagtcgctgt cgaagcggga gaattttgag ggcagccccg
     6421 tggattattt cgatttcggc tacgccctca ccgtgcacaa ggcccagggc agcgaggcgc
     6481 cggccgtggc ggtctttgtg gagaattttt ggtcgggaga gacgttgaag cgctggctct
     6541 ataccgccgt cacgcgggcc cgcgaacggc tgatcatcat tcgctagggg ggagactgac
     6601 atgagggcaa agaacaacga ggggaaggac gcggaggtct accgggccct gctcgaaaca
     6661 ccgtcgggga aaaactggga acgcctcggc ctcgcccgcc gcagcggcgt ggtggcgccg
     6721 ctgttttccg tgtactcggc cgacagcgtg ggcatcggcg agctggcgga cctggaccgc
     6781 ctcgccgact ggtgccgcgc cacgggcatg agcctcctcc aactcctgcc catgaacgac
     6841 gtcggtttcg attttcagcc gtacagcgcc cagtcctcct tcgcgctgga acccatgtac
     6901 ctctcgttca aacgcctcac gggggtgcgc ctctcgccgt tcctggagga catcgcggcg
     6961 ctgaaggggc gtttccccac cggccccggc cgcgtgaatt acggcatcaa aggcgcgaaa
     7021 ctggggctgc tctgggagat attccgggtc tcccaccgga aagggctcgc ttctttcgaa
     7081 aagttcgtgg agcagaaccg gttttggctg cacgactacg cggtcttcaa gacgttgaag
     7141 gaagtgttcg gacagagcgg ctgggagtcc tgggaggagc ctttccggcg gaaagacccc
     7201 gcggccctgg aacgtttcgc gcaggaacac gaacagcaga tcgagttcca gcagtggctg
     7261 caatggcagc tctacgaaca gttccgcgcg gccaaacgct acgcgcgcaa gcagggcgtt
     7321 ttcctcatcg gagacatccc cttcctcgtc tcccgcgaca gcgccgatgt gtgggccaac
     7381 cagtcctact tcaaactcca tctggcctcc ggcgccccgc ccgacatgtt cttcgccacc
     7441 ggccagcgct ggggcatgcc gccttacgac tggtcccgca tcgaggaaca ccgctacgac
     7501 tacgtggtgg agaaactccg ctacgccgaa cattttttcg atttctaccg catcgaccac
     7561 gtggtcggca tcttccgcgt gtggaccatc ccgctcagcg aaccggccga gaccggcgga
     7621 ttgaacgggg tgttcgaccc caaggacgag aacgaatggg aggaacacgg acgcaccttg
     7681 ctgcgggtga tgatcgacaa cacccgcatg atcccctgcg ccgaggacct gggggtggtg
     7741 ccgaaatgtt ccaacaaggt cttggaggaa ctcggcatcc ccggcatgga cgtgcagcgc
     7801 tggacgcgcg attacgaaaa gaccttcgac ttcaaggcgc ccgaggccta ccgctccgtg
     7861 gccctggcga cggtgggcac ccacgacacc tcctccctgc gcggctggtg gggcttcgag
     7921 gccgggacca tcgacgagat gcttttccgg cggaaatgcg cctcgcggaa catctcctac
     7981 gacgaggtgc gcgaccgcct tttcgacatg agccgcgccg cccacggccg cctgcgctgg
     8041 cggccggagg tgacggacgc ggagaccctg ctgaggatca tcgaccggcg cgaggaggag
     8101 gcgcgcgatt tcatctccct ctacaaggag gcctgcgacg aaaaggaacg attcctcgca
     8161 ttcgtcggcc tcgaccggcc ggccgattcg ccgggcgacg ccgccttcgt ccgggccgcg
     8221 ctccaaaaag ccggcgattc catcgc
//
LOCUS       DAOTFD010000106         8147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6226_length_8147_cov_4.093302, whole genome shotgun sequence.
ACCESSION   DAOTFD010000106 DAOTFD010000000
VERSION     DAOTFD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6226_length_8147_cov_4.093302"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="P5079_07005"
     rRNA            complement(<1..931)
                     /locus_tag="P5079_07005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1468..1824)
                     /locus_tag="P5079_07010"
     CDS             complement(1468..1824)
                     /locus_tag="P5079_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HRY29772.1"
                     /translation="MNLDEAMQAHVNWKMRLREYCRPNRREELDPAAVGKDNRCALGL
                     WIHGEGEGKYGNSSEFKNLVTAHAEFHRAASQVVELVNGGKVEEGLKILDDPQSTFRS
                     KTTEVIGLLSKLKKIQ"
     gene            1998..2552
                     /gene="rfbC"
                     /locus_tag="P5079_07015"
     CDS             1998..2552
                     /gene="rfbC"
                     /locus_tag="P5079_07015"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRY29773.1"
                     /translation="MRFLKTKLNGICLIEPLVHEDSRGFFFESYSQRDFEANGIQEHF
                     VQDNISRSRKGTWRGLHYQLPPHQQGKLVRVMEGEVFDVVVDIRRGSPTFGQWFGTPL
                     SAENKRALYVPPGFAHGFCVVSEVALLTYKCTALYSPLAERAIRWNDPKIGIKLPLEP
                     DPALISPKDKNAPSLDEAEISPFF"
     gene            complement(2559..3458)
                     /locus_tag="P5079_07020"
     CDS             complement(2559..3458)
                     /locus_tag="P5079_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY29774.1"
                     /translation="MKLGIFHFELLYNVLQDMHFLYNEEEMAEMVLTRISQALNAEAG
                     TIFRTEKDGTLYPLASYGVSKENLRKLTFKSGVGVVGWVAQYMQPVKVDRPETDPRFF
                     GTVDSASGFKTRCIIASPIIVRNELIGIIEFLNRRDGMFVLPDLELISMVGRQVGIAM
                     INARVIKERDDLLAFSEAVQGSLAAGLLVVDGQGKISKINRRAIDILGLGNTSSICGE
                     NAATTLIFALPLLKAIEETAVNSQPQTRQETTIRIQGREARIGYSTVPVIAKNGEKLG
                     VALLFQDISLWQPPAAKSTDEKN"
     gene            complement(3475..4386)
                     /locus_tag="P5079_07025"
     CDS             complement(3475..4386)
                     /locus_tag="P5079_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY29775.1"
                     /translation="MSVVVVGSVALDSVRTPFGERVNALGGSATYFSYSASFFTDVKL
                     VGVVGEDFPKEHLDLLRKKKVDLAGLQVVPGATFRWSGYYEYDLNEAKTLETHLNVFE
                     QFRPKLPDNYKDADCVFLANIDPDLQIDVLHQVRNPKLVAADTMNLWISTKKARIVEL
                     CRQIDILILNDGEARQLTGKPSLIKAGNEIRSWGPKYVVIKKGEHGVFLFCKDKFYAA
                     PAYPLEEVYDPTGAGDTFAGGMVGYLNKMGNKLDDRTMHEALFYGTVMASYNVENFSL
                     ERLRKLTPSDISQRVRKLRDMTSVQSA"
     gene            4635..5570
                     /locus_tag="P5079_07030"
     CDS             4635..5570
                     /locus_tag="P5079_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY29776.1"
                     /translation="MGLLRPLAIYYQTKMKLAKLRIHLLPRRGDRVLDVGSGDGPTPA
                     ADVLCDRFVGDDAERTAPLKLDRPFVSGDVQDLPFADGAFDFVYCSHLLEHTTDPARA
                     IAELQRVAKAGYIEAPSEYLEKAAKSTAAHLWFVRQENRTLVFSPKPAGTLDTLVNVV
                     FDELLMYKDDLYTAFHWSRFYDLFNIRFVWRGTIPHRIEGKARPADAFLKGKESLPSA
                     ERIARLREMFKAARAGDSAALSLAKGFLKKLIRLYYATGKKFDHLDLLACPVCKKPLQ
                     RSTELQRFTCVSCRTWYPWIEGVPCLIRAAAQDLA"
     gene            5570..7000
                     /locus_tag="P5079_07035"
     CDS             5570..7000
                     /locus_tag="P5079_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRY29777.1"
                     /translation="MSLRQRITRNATFKVLGEAGGRLAGMLFYILFARWLGAQEFGRY
                     SFALSYAALFAIVVDLGTNTIITRELARDRSLTGEFVPRLNALKTFSSVLALVLIGVS
                     LLFFSYDLGTKKLVLAMGLFVIGTGLLEYFCAVLSGLERMGREAFLKTANKLLVLAGG
                     AAGFFLYRTLASTVAGLLLGMGLSLVLGYGFLFRQGAPAALRWNWPWAKKLLAQSLPL
                     LASWVFWNFYDNQDIVLLSFLGRPMNEVGWFAAAMKLLDVFRGVPVLMAGAFFPVLAN
                     FARTDPDHFTRLARFLMRWIVAVALPLAAGTAMLAGPIVAFIYGADFLFSARILTIAV
                     WAAVGIFINHVFINLLVALDMQNKTIVGAAVAAGTNLLLILVLVPWGGLLGAAAALPL
                     SEAVYLVINSHFTHKRVTVFDGPFWRYLLKPVAATALMAATLWFLPSWNVFILAAVGA
                     AVYAAALLALRGVPTKVSALADPATI"
     gene            7005..7925
                     /locus_tag="P5079_07040"
     CDS             7005..7925
                     /locus_tag="P5079_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29778.1"
                     /translation="MTAATLSIVIPNYNGRELLERCLASIHKNPPACPFDVVVVDNAS
                     SDGSADAVRRLFPQVRLIANPDNRGFGPAANQGLRGAAAPYLFLLNSDTEVTPGALDT
                     LVQGMEQNRDVAVMAPEFTDGRGALIQMSWGWHSLFWGELPQKFFSPKALQKSAFRRW
                     LVKRLQTRPKRTPIVCGAGLLLRRQALDEIGLLDERFVMYFEESDLCLRAWKNGWAVL
                     FYPRAKVVHHLGKSSGETPGKLSLVYRQSQLYYYDKHGTALQRRLVRLYLRLKFWRIV
                     LYKIFPRLCSDPDYYSQMDAVLKGTTRVSL"
     gene            7922..>8147
                     /locus_tag="P5079_07045"
     CDS             7922..>8147
                     /locus_tag="P5079_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29779.1"
                     /translation="MNNRSLIALDARMAEHSGIGSYIRGLVTHFSPEQKDRLVLLGDP
                     HQLSRYGRPVISAHESIYGVWEQWAMPGRLR"
BASE COUNT         1519 a         2549 c         2294 g         1785 t
ORIGIN      
        1 gggcccccgt caattccttt gagtttcaac cttgcggccg tactccccag gcggctcact
       61 taatgcgtta gcgacggcgc ggaaggggtc gatacctccc acacctagtg agcatcgttt
      121 acagctggga ctaccagggt atctaatcct gtttgctccc ccagctttcg tggatgagcg
      181 tcaatagtgg tccagtgacc tgctttcgcc atcggtgttc ctcctgatat ctacgcattt
      241 caccgctaca ccaggaattc cagtcacctc tcccacattc aagtccggca gtatccattg
      301 gcctctcccg gttgagccag gagctttcac aacagactta ccgaaccacc tgcccacgct
      361 ttacgcctag taattccgag taacgctcgc caccttcgtc ttaccgcggc tgctggcacg
      421 aagttagccg tggcttattc gcagagtacc atcaaaacgg gctcttcacc cgtccatttt
      481 tcccctgcaa aaggagttta caatccgaag accttcgtcc tccacgcggc atcgctgcgt
      541 cagggtttcc cccattgcgc aaaattcccc actgctgcct cccgtaggag tatggcccgt
      601 gtctcagtgc cattgtggcc ggtcgtcctt tcagaccggc tacccgtcgt cgccttggtg
      661 ggccgttacc ccgccaacta gctgataggc cgtgaactcc tcttcaagcg tcccttgcgg
      721 aactttagcc tcgtccccat gcggggacgt ggtcttacgc ggtattagcg ccggtttccc
      781 ggcgttatcc ccctctcaaa ggaagattgt tcacgtattg ctcacccgtc tgccactaac
      841 ttcggcgatg aactgccgaa gcccgttcga cttgcatgtg ttaggcatgc cgccagcgtt
      901 cactctgagc caggatcaaa ctctccgtta aattcgcacg ccggccacga cccttgcgga
      961 ccgggacacg acgcaaaaat tttggagtgt tttttaatcg gctctgaatt gagggttgtc
     1021 catgcggaca atccttgtgt gttttcacac caaactactg tcgtttgtgc aaaaggctcc
     1081 gagggctacg gacgccccaa accacggggc gctcacccgc tcgcgccttt tgactcatca
     1141 ggaatgtcaa agaacaatcg tagcgcgaaa aaaacaaacc cagaagggcg aaaaactccc
     1201 ccttcccaag agaccccttc ggtctctctt ggcaccggca acatcggccg gctcgcacta
     1261 cctatgagga tttgccccaa accccgtatg gtttagggga tccgaaacag attctagcag
     1321 agccctctgg aacatgtcaa cacttttttt gaattttttt tattttttta tccgccgctt
     1381 ttgggcgtcg gtccatgccc cgacttccgc ttattctccc gacggaaaaa cagaccggcg
     1441 ttgccggccg gcggatggcc gccgccctta ttggatcttt ttcaatttag acaacagtcc
     1501 gatcacttcc gtggttttgc tgcggaaagt cgattgcggg tcatccagga ttttcaatcc
     1561 ctcctccacc ttgcccccgt tgacaagttc aacaacctgg gacgcggccc tgtgaaactc
     1621 ggcatgggcg gtcaccagat ttttaaattc ggaagagttc ccatacttcc cctccccttc
     1681 cccatggatc cacaatccca aagcacagcg gttgtccttg cccaccgcgg ccgggtccaa
     1741 ttcctcccgt cgattcggcc tgcaatactc cctcaaccgc attttccagt tcacgtgcgc
     1801 ctgcatcgct tcatccaggt tcatcggtta ttcctccctc gtcgaaaact ctgccccctc
     1861 attaaactta ttttatcatt ttctaggaaa cgccgcccgc aaaaaatccc ttgccggtct
     1921 ttccgttcgg cgttataatc tcccacatct gtttttatcc gtttatcttg tgcgacccgg
     1981 aggagtttcc ctcggaaatg agatttctaa aaacaaaact gaacggcatc tgcctcattg
     2041 agcccctggt ccacgaggat tcccgtggat tttttttcga gtcctacagc cagcgggatt
     2101 tcgaggccaa cggcatccaa gagcacttcg ttcaagacaa catcagccgg tcccgcaaag
     2161 gcacctggcg cggactgcat taccagctcc ctcctcacca acagggaaaa ctcgtccggg
     2221 tcatggaagg ggaggtcttc gacgtggtgg tggacatccg tcggggatct ccgaccttcg
     2281 gccagtggtt cgggaccccc ctctccgccg agaacaaacg ggccctctat gtgccgccgg
     2341 ggttcgccca tgggttctgt gtggtgagcg aggtcgccct tcttacctat aagtgcaccg
     2401 cgctgtattc ccccctggcc gagcgggcca tccgatggaa cgatcccaag atcgggatca
     2461 agctccctct ggagccggat ccggccctca tctcccccaa agacaaaaac gccccgtcgt
     2521 tggacgaggc ggaaataagt ccgttttttt aatccggatc agtttttttc gtcggtggac
     2581 ttggcggcgg gcggttgcca aaggctgatg tcctgaaaaa gcaacgccac tcccaacttt
     2641 tctccatttt tggcaatcac cggcaccgtt gaatagccga tacgggcctc ccggccttga
     2701 atgcgaatgg tcgtctcctg gcgggtctgg ggctgagaat tgacagcggt ctcttcaatg
     2761 gccttcagca gcggcagggc aaaaatgagc gtcgtggctg cattttcgcc gcagattgac
     2821 gatgtgtttc ctagtccaag gatatcgatg gcacggcggt tgatcttgct gattttcccc
     2881 tggccgtcca ccaccagcag ccccgccgcg agactcccct gaaccgcctc tgaaaaagcc
     2941 aacaaatcgt cccgttcctt gatgacccgg gcgttgatca tggcgatgcc cacctggcgg
     3001 cccaccatgg agatcagttc cagatccggc aagacgaaca tgccgtcgcg gcggttcaaa
     3061 aactcgatga taccgatgag ttcgttgcgg acgatgatgg gagaagcgat gatgcagcgc
     3121 gtcttgaacc cgctggcgct gtccaccgtg ccgaaaaacc gcgggtccgt ctccggacgg
     3181 tccactttca ccggctgcat gtattgcgcc acccatccca ccacccccac gccggactta
     3241 aacgtgagct tgcgtagatt ctccttggaa acgccgtagg aggccagggg gtagagcgtt
     3301 ccgtcctttt cggtacggaa gatggttccc gcctcggcgt tgagcgcctg cgagatgcgc
     3361 gtcaagacca tctccgccat ttcctcctcg ttgtagagga agtgcatgtc ctggaggacg
     3421 ttgtacagca gttcgaaatg gaagatgccg agtttcacgt ttttcccttc cccgtcaggc
     3481 ggactgaacg gaggtcatgt cgcgcaactt gcgcacgcgt tgggagatat ccgacggggt
     3541 aagttttctg aggcgctcca gcgagaaatt ttccacgttg tagcttgcca tcacggtgcc
     3601 gtagaaaagc gcctcatgca tggtcctgtc gtccagcttg ttccccatct tgttgagata
     3661 tccgaccatc cctccggcaa aggtatcgcc ggcgcccgtc gggtcataga cctcctccag
     3721 cgggtaggcc ggggcggcgt aaaacttgtc cttgcaaaag aggaagacgc cgtgctcccc
     3781 cttcttgatc accacgtatt tcggccccca ggagcggatc tcgttcccgg ccttgatgag
     3841 gctgggtttg ccggtcaact gccgcgcctc gccgtcgtta aggatgagga tgtcgatctg
     3901 gcggcagagt tccacgatgc gcgccttttt ggtggaaatc cagaggttca tggtgtcggc
     3961 ggccaccagt ttggggttgc gcacctgatg gagcacgtcg atctgcaggt cgggatcgat
     4021 gttggccagg aaaacacagt cggcgtcctt gtaattgtcc ggcagtttgg gacggaactg
     4081 ttcgaaaacg ttaaggtgcg tctccagggt cttggcttcg ttgaggtcgt attcgtaata
     4141 gcccgaccag cggaacgtgg cgcccggcac cacctgaaga ccggcgaggt ccaccttctt
     4201 tttgcgcagg agatccagat gttccttggg gaaatcctcc cccaccacac cgaccagttt
     4261 cacgtcggtg aaaaagctgg ccgaatagga gaaataggtg gccgaaccac ccagcgcgtt
     4321 gacgcgctcg ccgaacgggg tgcgcacgga atccaaagcc acggaaccga caacgacgac
     4381 gctcatggtt tatacctctc attttcctga aagattttcc cgggaacagg ggccgaaact
     4441 tcgcccgtcg aattgtagca aaaaagagaa aatcgtcgga gggttttccg ccgcgaagag
     4501 gccggcgccg ctccggatct ccggcaaaaa cagctatgaa aaaaaagtgc gtcttgttac
     4561 aatagaatgc tttgggccgt ctctctgacg gcgtttttaa aatcctacgc cctccctcaa
     4621 ctacaaggag acacatggga ctgcttcgcc ctctggcgat ctactatcag acgaagatga
     4681 aactggccaa actgcggatc catcttctgc cgcggcgcgg cgatcgcgtg ctggacgtgg
     4741 gcagcgggga cgggcccacc ccggcggcgg acgtgctctg cgaccgtttt gtgggcgacg
     4801 acgccgaacg caccgctccc ctgaaactgg accgtccctt cgtctccgga gacgtgcagg
     4861 acctgccttt cgcggacggg gccttcgatt tcgtgtattg cagtcacctg ttggaacaca
     4921 ccacggaccc ggcgcgggcc atcgccgaac tccaacgcgt ggcgaaagcc ggatacatcg
     4981 aagccccttc ggaatacctg gagaaagccg ccaaaagcac cgccgcccat ctgtggttcg
     5041 tgcgccagga gaaccgcacg ctggtctttt ccccgaagcc cgcggggacg ctggacactc
     5101 tcgtcaacgt ggtcttcgat gaacttctga tgtacaagga cgacctctac accgccttcc
     5161 actggtcgcg gttctacgat ttgttcaaca tccggttcgt ctggcgcgga accatccccc
     5221 accggatcga aggcaaggcg cggccggcgg acgccttttt gaaggggaag gaatccctgc
     5281 cgtcggcgga acgcatcgcc cggctgcggg agatgttcaa ggcggcccgg gccggggact
     5341 ccgccgcgct gtccctggcg aaagggttcc tcaagaaatt gatacgcctt tattacgcca
     5401 ccggaaaaaa attcgaccac ctcgacctcc tcgcctgtcc cgtttgcaaa aaacccctgc
     5461 aacgttcgac ggaactccag cggttcacct gcgtctcctg ccgcacgtgg tacccctgga
     5521 tcgagggcgt tccctgcctg atccgcgcgg cggcgcagga cctcgcgtag tgagtctacg
     5581 acagcgcatc acccgcaacg ccaccttcaa agtcctgggc gaagccggcg gccggctggc
     5641 cgggatgctt ttctacatcc tgtttgcgcg ctggctgggc gcccaggaat tcggccgtta
     5701 ttccttcgcg ctctcctacg ccgcgctgtt cgccatcgtg gtggacctgg gaaccaacac
     5761 catcatcacg cgggaactgg cgagggaccg ttccctcacc ggagagttcg tgccgcggtt
     5821 gaacgcgttg aagacttttt cctccgtatt ggccctggtc ctcatcggcg tgagcctcct
     5881 gttcttctcc tacgacctcg gcaccaaaaa actggtgctg gccatggggc tgttcgtgat
     5941 cggcacgggg cttctggaat atttttgcgc ggtgctttcc ggcctggaac ggatgggacg
     6001 ggaggcgttc ctcaaaaccg ccaacaaact gctggtcctg gcgggcggcg ccgcgggttt
     6061 cttcctttac cgcacgttgg cgtccacggt ggcgggtctc ctgctgggaa tggggctgag
     6121 cctcgtgctc gggtacggat ttttgttccg acagggcgcg ccggcggcgc tgcggtggaa
     6181 ctggccctgg gcgaaaaaat tgttagctca gagcctgccc ctgctggcgt cgtgggtgtt
     6241 ttggaatttc tacgacaacc aggacatcgt cctcttgtcc ttcctggggc ggcccatgaa
     6301 cgaggtgggg tggtttgcgg cggcgatgaa actgctcgac gtgttccgcg gagtcccggt
     6361 gttgatggcc ggggcgttct tcccggtgct ggcgaacttc gccaggaccg atccggacca
     6421 cttcacgcgg ctggcccggt tcttgatgcg ctggatcgtg gcggtggccc tgccgctggc
     6481 ggccggcacg gcgatgttgg cgggccccat cgtggcgttc atctacggcg cggacttttt
     6541 gttcagcgcg cgcatcctga cgatcgccgt gtgggccgcg gtgggcatct tcatcaacca
     6601 cgtcttcatc aacctgctgg tggctttgga catgcagaac aaaaccatcg tgggggcggc
     6661 ggtggcggcg ggaacgaacc tcctgctgat cctggtgctg gtgccgtggg gcggcctgtt
     6721 gggggcggcg gcggcgctgc ccctgagcga ggcggtgtat ctggtcatca actcccattt
     6781 cacccacaaa cgcgtgacgg tttttgacgg cccgttttgg cggtatctcc tcaaacccgt
     6841 cgcggccacg gcgttgatgg ccgctaccct gtggttcctt ccctcctgga acgtgttcat
     6901 cctggccgcg gtgggcgcgg cggtctatgc cgccgcgctc ctggcgcttc gcggcgtgcc
     6961 cacgaaagtg tccgccctgg ccgacccggc cacgatctga tcccatgacc gccgcgacgc
     7021 tctccatcgt cattcccaac tacaacggcc gggaactcct ggagcggtgc ctggcgtcca
     7081 tccacaaaaa cccgcccgcg tgtccttttg acgtcgtcgt ggtggacaac gcctcctcgg
     7141 acggcagcgc cgacgcggtg cggcgtctct tcccgcaagt cagactcatc gccaacccgg
     7201 acaaccgggg gttcggaccg gcggccaacc agggcctgcg cggggcggcc gcgccttacc
     7261 tttttcttct caacagcgac acggaggtga cgccgggcgc cctggacacg ctggtgcagg
     7321 ggatggaaca gaaccgcgac gtcgcggtca tggcgccgga attcaccgac gggcgcggcg
     7381 cgctcatcca gatgtcgtgg ggatggcatt ccctgttctg gggcgagttg ccgcagaaat
     7441 tcttttcgcc gaaagccctg caaaaaagcg ccttccggcg ctggctggta aaacgcctgc
     7501 agacgcggcc gaaacggacg cccatcgtct gcggcgcggg gctcctgctg cgacgccagg
     7561 ccctggacga aatcggactg ctcgacgaac ggttcgtcat gtatttcgag gagtccgacc
     7621 tgtgcctgcg cgcctggaaa aacgggtggg cggtgctctt ttatccacgg gcgaaggtgg
     7681 tccaccatct ggggaaatcc tccggcgaaa ccccgggcaa gctttcgctt gtctatcgac
     7741 aaagccagtt atactactac gacaaacatg ggaccgccct gcagcggcgg ctggtgcggc
     7801 tttatctgcg gctgaaattc tggcgcatcg tcctctacaa gatcttcccg cgcctctgtt
     7861 ccgaccccga ctattacagt caaatggacg ccgtgctcaa aggcacgacg cgggtctccc
     7921 tatgaacaac cgctccctca tcgccttgga tgcccgcatg gcggagcatt ccggcatcgg
     7981 cagttatatc cgcgggctgg tgacccattt ttcgccggaa cagaaggacc ggttggtcct
     8041 tctgggcgat ccgcaccaac tgagccgtta tggacgaccg gtcatctccg cccatgaatc
     8101 catttacggg gtgtgggaac aatgggcgat gccgggccgg ctgagga
//
LOCUS       DAOTFD010000107         8142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6229_length_8142_cov_4.421664, whole genome shotgun sequence.
ACCESSION   DAOTFD010000107 DAOTFD010000000
VERSION     DAOTFD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_8142_cov_4.421664"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="P5079_07050"
     CDS             complement(<1..708)
                     /locus_tag="P5079_07050"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRY29780.1"
                     /translation="MRMTLSEIAKLIGGAVAGDGNVVIEGAASLAEATEKEISFLGNA
                     KYTGQLKATRAGAVILPPGVDPGGKNAVVVKNPPYGWARVLELLEKERLRHPTGIHPT
                     AVIAKTARLGAGVTVGAHTIIEENAQVGDNTVIYPHCYIGRDSRVGKNGLLYPRVVIR
                     ERVTVGDRCIFQPGVVLGADGFGFALHEGRHYKIPQVGTLEIGDDVEIQANSTVDRAA
                     TGKTRIGRGTKIDNLVQV"
     gene            complement(722..1438)
                     /locus_tag="P5079_07055"
     CDS             complement(722..1438)
                     /locus_tag="P5079_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRY29781.1"
                     /translation="MKKTLIALPLLGFIALSSHVSALEIPLGRGGSGGGSTVGYVDME
                     AVFQEYPETQKAKEEYYRELADKRAVLADREKELADLKEQLSILRATLKEMQAGMAQA
                     AAAASTETVTENSEETSTDTAAGEPPAKSLAMDLGISSGSITAVNETLVQREKALAEK
                     ETGLEEARRQAVQSLKEFEERRSLQIFGKLYSALVQLAEEQGISLVVDKNSILYGQNA
                     MDLTEKLRRRVRGLPDDAAK"
     gene            complement(1528..3855)
                     /gene="bamA"
                     /locus_tag="P5079_07060"
     CDS             complement(1528..3855)
                     /gene="bamA"
                     /locus_tag="P5079_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRY29782.1"
                     /translation="MTPSPVSRLRLRLGAACLLAVTLLFLKPMRLPALETRQMIAEVT
                     VEGNQSVKKRTVLGKVKIRKGDVVVDPAFRADVDRLLETGLFDDVQVALEDAPGPTDA
                     QGLARVRVIFRVKERSIIKRVDFKGNLKLPARKFRDELASKPGEPHDRFKVAQDVQKI
                     LSIYRDEGYADAKVEYFTSLDPKKRKTILTFFMTEGDRVMIKAVEVVGNRTVSAGKIR
                     RLMKKTRRKKVFKEDSLREDLDEVKKFYQNKGFLEVSLSPPARDFNEDHTRVSLTLNI
                     VEGRRYRVGQFTFEGATLFDQKTLRKAITLKSGKLYRQDRMDESLQNIQNLYADKGYL
                     RAEIVPNNITQAFDDDNGQVDIRFDITESSVVYVDRIYIDGNTYTKEQVIRREILLKE
                     GDVFAAGRVRRSVEKIYNLGFLDDVQVDVQQPRSPTRADIVFSVMEGKPGILSAGAGF
                     SSVDGLLGTLQLQHINLFGRAQRLNLLWEFGERKQNYEIGWTDPWFLGKRMSFGVSVF
                     DTVRRRPFGTDSTAYREGRRGFSLQLGPRITEQLSLIHNYSYEEVRIYDIDSEHINDI
                     SPSEDITSSLTNGIVLDTRDNIFDASRGMRNSASVQVAGGPLGGDMNFYKPQLSSALY
                     VPTFWKFVFSLAGRGGYVRHFAPSKEVPISERFVMGGVDTVRGYDFGEIGPEDYGRVM
                     MVFNAEYKFPIVQERNRTILQGAFFADAGGSWSHADQVDLRIGRQENQMRSGVGFGIR
                     FKTPVFPIRLDWGYGLNHRPGEQISQFYFTIGNIF"
     gene            complement(3852..8090)
                     /locus_tag="P5079_07065"
     CDS             complement(3852..8090)
                     /locus_tag="P5079_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY29783.1"
                     /translation="MRDAKWAGIALPSLTVSLQGHPGRWEGETSFLNGRLLFSQEPLP
                     DPSTPTEEVRSLWTLRANGLEMGEWARLNRWGNISGTVSGKARLRKGPAFDLRTLEAD
                     GFLKVDRFAWGVHKETAPISGRLSFKKGQLLIQDLSNLFRLNASFSPEKTVLSELELS
                     LPSGMTLSANGTVEGPERTLSLQASVKRMPVRDLPMLLERYPEISGSIDFDGRLSGTA
                     AAPVFTGTGDLNVLKLRRTSDPVDAHTDVHWTNEEFALSPWRVGSAWEGQLRWADRHG
                     WDARLKAVDGDARFLGDLILLDAPLEGKINGDFHLNVSQKGVSNWEGTADVTWTRGKW
                     GSLPFDKVRGAAKISGGVLSLKGFDVSQPSGSVHLEGALPLGKKTTVPSSFTAQFKHF
                     VLPSLTLDGELHVSAGDTTVTGRPHGEFRSPNFWVNGIGLGSASGKFQWDGTDLRLAE
                     LRIGEDLRAEVSLNPGQRSLRGQLLVQEAEMSAWAPRWGMAHPLLRSGRVNAVLNWEG
                     PWKAPRNTFRITWKNAAWTGAPFDISMKGSWDGQRVELEDIHAGPSGQPASLAGRGTV
                     SFDAEKRSAPQIDLAVNLTGFPVHHLFGAQPDWKLPVDGRFTWRGPLTRPVLSGTFSG
                     GENNFGNFMLRKWQGTVQWQNKELQFSDFTVETPDGFFRLKNGSRILMTQAGQGHFQM
                     LGDMRNVHLGPLTFFGGMELAGQWKNSPVPRIDCNLRALSLWVNQHRFDQDLARLSWS
                     GRELRFTPVPGTLQQLAGAVHFNQWPQLHFENLTLTELGQRRFWVQGEIGPQRWEFDV
                     EGLGLDAETVIGLADMDFPVDGSLNVRVKGKGSPQAPEIEGDIIGEKGRFGRLPFDLA
                     TAHVYWNGSLLEISHLEAARKGRYLLKGGGRIPVSSDESETAKGMSFSVQIANGDMAV
                     VQDLWPDCAVAKGSFHGELRLRPGVGGMQTSGHLVVENAEMKARKYFREITDLNARLL
                     IRDNRLVFENLSARVGHGRLLIRGDLGLDGFDITDYNLTAESLGRPGISIEIPQLAVP
                     PGPLLKRFSLLRESLENVSRGSPMVYLALTGPDGEQTLRGTIILEDTQFTYPPAKKVA
                     LKSGSWYYDFWNELNWDVLFKTADDTWYRNEFVNVQVKGNVRVFGPRKDIRAAGRIDT
                     SRGVISYLGQSYQIKQGGFEVVTDTRAAVADRATVPYLWTEADRAAIILDRGVPTPDT
                     ITMIVDRAPLGELQPRFLSRNNPGWSSDQVAKKTLGLSEDQFTPLERDQLLRAGLVQL
                     LGSTAGPLASRIAQRFGIDILYPIYEPEDSQLVQTPARATPVPRGDILNLLQGTGASA
                     GMQLSNRVFGLYKFKMDQMQNQLFFRDEIELVYRIRGNLHLRASTELDTEKLLGQPPN
                     RQAILENQWRFGQPKPPAPKDKNQPTPPSGQPTR"
BASE COUNT         1355 a         2402 c         2712 g         1673 t
ORIGIN      
        1 gacctgcacc aggttgtcga tcttcgtgcc gcgtccgatg cgggtcttcc ccgtggcggc
       61 gcggtccacg gtgctgttgg cctggatctc cacgtcgtcg ccgatttcca gggtgcccac
      121 ctgcgggatc ttgtaatggc ggccttcgtg gagggcgaaa ccgaacccgt ccgccccgag
      181 caccaccccc ggctggaaaa tgcagcggtc tccgacggtg acgcgttcgc ggatgaccac
      241 gcggggatag aggaggccgt ttttccccac gcggctgtct cggccgatat agcagtgcgg
      301 atagatcacg gtgttgtcgc cgacctgggc gttctcctcg atgatcgtgt gggcgccgac
      361 ggtcacgccg gcgccgaggc gggcggtctt cgcgatgacg gccgtgggat ggatgccggt
      421 cggatggcgc aggcgttcct tttccaaaag ttccagcacg cgggcccagc cgtagggagg
      481 gttcttgacg acgaccgcgt ttttcccgcc ggggtccacg ccgggcggga ggatgaccgc
      541 cccggcgcgc gtggccttga gctgcccggt gtatttggcg ttgccgagga acgagatctc
      601 tttctccgtg gcttcggcca ggctggcggc gccctcgatg acgacgttcc cgtcgcccgc
      661 cacggcgcct ccgatcaatt tcgcgatttc cgataacgtc attcgcatgg aaagccgctc
      721 cttatttggc cgcgtcgtcc gggaggccgc gcacgcgccg ccgcagtttt tcggtcagat
      781 ccatggcgtt ctggccgtag aggatgctgt tcttgtccac caccaggctg atgccctgtt
      841 cctcggccag ttgaacgagc gcgctgtaga gtttcccgaa gatctgcagg ctgcgccgtt
      901 cctcaaattc tttcagggat tgcaccgcct gccggcgggc ctcttccagc ccggtttctt
      961 tttcagccag ggccttctcc cgttgcacca gggtttcgtt gacggccgtg atggaaccgg
     1021 aactgatgcc caggtccatg gccaggctct tggcgggcgg ttccccggcg gcggtgtcgg
     1081 tggaggtttc ttcgctgttt tccgtgacgg tctcggtgga ggccgccgcg gccgcctggg
     1141 ccatgccggc ctgcatctcc ttcagggtgg cccgcaaaat ggagagttgt tccttcaggt
     1201 ccgccagctc cttttcccgg tcggccagga cggcgcgttt gtcggccagt tcccggtaat
     1261 attcctcctt ggccttttgt gtttcgggat attcctggaa aacggcttcc atgtccacgt
     1321 aaccgacggt gcttccgccg cccgacccgc cgcggcccag agggatttcc aaggcggaga
     1381 cgtgcgagga cagggcgatg aatcccagca ggggaagcgc gatgagcgtt tttttcattg
     1441 ttttcttttt attgagtggc ttggcggccg tcgaacgcaa ggcactattt tacacaaact
     1501 ccggcgcggt gtcgagcgtc cggacgctta aaaaatgttt ccgatggtga aatagaactg
     1561 gctgatctgc tctcccggcc ggtggttcag gccgtagccc cagtcgaggc ggatcgggaa
     1621 aacgggcgtt ttgaagcgta tgccgaaccc cacgccggaa cgcatctggt tctcctggcg
     1681 gccgatgcgg aggtccacct ggtcggcgtg gctccagctt ccgccggcgt ccgcgaagaa
     1741 ggcgccctgg aggatggtgc ggttccgttc ctggacgatg gggaacttgt attccgcgtt
     1801 gaagaccatc atcacgcgcc catagtcctc cgggccgatc tctccgaaat cgtagccgcg
     1861 cacggtgtcc acgccgccca tgacgaagcg ttccgagatg ggcacttcct tggacggggc
     1921 gaagtgccgg acgtaaccgc cgcggccggc gaggctgaag acgaacttcc agaacgtcgg
     1981 gacatagagg gcgctggaaa gctggggttt gtaaaagttc atgtcccctc ccagcggtcc
     2041 gccggccacc tgcacagagg cgctgttccg catgccgcgg gaggcgtcga agatgttgtc
     2101 gcgcgtgtcg agcacgatgc cgttggtgag gctggaggtg atgtcctccg acggggagat
     2161 gtcgttgatg tgttcggaat cgatgtcgta gatgcggacc tcttcgtagc tgtagttgtg
     2221 gatgagagaa agctgttcgg tgatccgcgg tccgagttgg agggagaatc cgcgccggcc
     2281 ttcccggtag gcggtgctgt ccgtgccgaa ggggcgtcgg cgcacggtgt cgaacacgct
     2341 gacgccgaag ctcatgcgtt tgccgaggaa ccacgggtcg gtccagccga tctcgtaatt
     2401 ttgtttgcgt tcgccgaact cccacaggag gttcagccgc tgggcccggc cgaagaggtt
     2461 gatgtgctgg agctgaaggg tgccgaggag cccgtccacg ctggaaaaac cggcgccggc
     2521 ggagaggatg cccggtttgc cctccatcac ggagaagacg atatcggcgc gcgtgggaga
     2581 acgcggctgt tgcacgtcca cctgcacatc gtccagaaaa ccgaggttgt atatcttttc
     2641 cacggagcgg cgcacccggc cggcggcgaa aacgtccccc tctttcagca ggatctcccg
     2701 gcggatcacc tgttccttgg tgtaggtgtt cccgtcgatg tagatgcggt ccacgtagac
     2761 cacggaactt tcggtgatgt cgaagcggat gtccacctgg ccgttgtcgt cgtcgaaggc
     2821 ctgggtgatg ttgttgggaa cgatctccgc ccgcaggtag cccttgtcgg cgtaaaggtt
     2881 ctgaatgttc tgaagggact cgtccatgcg gtcctggcgg tagagtttcc cgctcttcag
     2941 ggtgatggcc ttccggaggg tcttttggtc gaagagggtg gcgccttcaa aggtgaactg
     3001 gcccacgcgg taccgccggc cttcgacgat gttcagcgtg agagagaccc gggtgtggtc
     3061 ctcgttgaag tcccgggccg gcgggctgag ggagacctcc aggaacccct tgttctgata
     3121 gaacttcttg acctcgtcga ggtcttcccg caggctgtcc tccttgaaca cttttttgcg
     3181 gcgggtcttt ttcatcaggc ggcgtatctt gccggcggag acggtcctgt tgccgacgac
     3241 ctcgacggcc ttgatcatca cccggtcgcc ctctgtcatg aagaacgtca ggatggtttt
     3301 gcgttttttg ggatcgagcg atgtgaaata ttcgaccttg gcgtcggcgt acccttcgtc
     3361 ccggtagatg ctgaggatct tttggacgtc ctgggccacc ttgaaacggt cgtggggttc
     3421 tccgggtttg gaggcgagtt cgtcgcggaa tttgcgggcc ggcagtttga ggttcccttt
     3481 gaaatccacc cgtttgatga tggagcgttc tttcacacgg aagatgacgc gcacccgggc
     3541 caggccctgg gcgtcggtgg gaccgggggc gtcctccagg gccacctgaa cgtcgtcgaa
     3601 gaggccggtt tccaggagac ggtccacatc ggcccggaag gcgggatcca ccaccacgtc
     3661 gcccttgcga attttgactt tgccgaggac ggtgcgtttc ttcaccgact ggttcccttc
     3721 caccgtcact tcggcgatca tttgccgggt ttccagggcc gggaggcgca tcggtttcaa
     3781 aaagaggagg gtgacggcca gaaggcaggc cgcgccgagg cgaaggcgga gacgggagac
     3841 gggagacggg gtcatcgtgt tggttggccg gagggagggg tcggctggtt tttgtccttt
     3901 ggcgcgggcg gtttcggctg gccgaaacgc cactggtttt ccaggatggc ctggcggttg
     3961 ggcggctggc ccaggagctt ttcggtgtcg agctccgtgc tggcgcgcag gtggaggttc
     4021 ccccggatgc ggtagacgag ctcgatttcg tcgcggaaaa aaagctggtt ttgcatttgg
     4081 tccattttga atttgtagag tccgaagacg cggttgctca gctgcatgcc ggcggaggcg
     4141 ccggtgccct gaagcaggtt gaggatgtct ccccgcggca cgggggtggc gcgggcgggc
     4201 gtctgtacca gctggctgtc ttcgggttca tagatgggat ataggatatc aattccgaag
     4261 cgttgggcaa tacggctggc gagcggtccg gcggtggagc cgaggagttg gacgagcccc
     4321 gcgcgcagca gctggtcccg ttccagcgga gtgaactggt cttcggacaa tcccagcgtc
     4381 ttcttggcga cctggtcgga actccatccg gggttgttgc gggagagaaa acggggctgg
     4441 agttcgccga gcggggcgcg gtccacgatc atggtgatgg tgtcgggcgt ggggacgccc
     4501 cggtcgagga tgatggccgc ccggtccgct tccgtccaga gataggggac ggtggcgcgg
     4561 tcggccacgg cggcgcgggt gtccgtcacc acttcaaacc cgccctgttt gatctggtag
     4621 gattggccca gatagctgat gacgccgcgg gaggtgtcga tccgtccggc cgcgcggatg
     4681 tctttgcggg gaccgaacac ccgcacgttt cccttgacct ggacgttgac gaactcgttg
     4741 cggtaccagg tgtcgtcggc ggttttgaag agcacgtccc agttcaactc gttccagaaa
     4801 tcgtaatacc atgaaccgct cttcagcgcc acctttttcg ccggcgggta ggtgaattgg
     4861 gtgtcttcca gaatgatcgt gccgcggagc gtctgttcgc cgtccggacc ggtgagggcg
     4921 agatagacca tcggactgcc gcgggagacg ttctccaggg attcgcggag gagagagaat
     4981 cgtttgagaa gggggccggg cggcacggcc aactggggta tctcgatgga aatgccgggg
     5041 cggccgaggg attccgccgt gaggttgtaa tccgtgatgt cgaaaccgtc cagcccgaga
     5101 tctccccgga tgaggagtcg cccgtggccg actcgggcgg aaaggttttc gaacaccagc
     5161 cggttgtctc ggatgagcag tcgggcgttg aggtcggtga tctcccggaa atatttgcgg
     5221 gctttcatct ccgcgttctc caccacgagg tgtccgctcg tttgcatgcc gccgacgccg
     5281 gggcggaggc ggagttcgcc gtggaacgat cctttggcga cggcgcagtc cggccagagg
     5341 tcctggacga cggccatgtc cccgttggca atctgcaccg agaaggacat ccccttggcg
     5401 gtttccgatt cgtcggaaga gacggggatc cttccgccgc ctttcagaag gtagcgtcct
     5461 ttccgagccg cttccaaatg gcttatttcc agcagggagc cgttccagta aacatgggcg
     5521 gtggcgagat cgaagggcag ccgcccgaac cggccttttt caccgatgat gtccccctcg
     5581 atctccgggg cctggggaga gcctttgccc ttgacccgga cgttcagcga accgtcgacg
     5641 gggaaatcca tgtccgccaa gccgatgacg gtctccgcgt ccaggccgag cccttccacg
     5701 tcgaactccc agcgttgcgg cccgatctcg ccttgcaccc agaaccggcg ctggcccagt
     5761 tcggtcaagg tcaggttttc gaagtgcagt tgcggccact ggttaaaatg aaccgcgccc
     5821 gccagctgtt gcagggtccc cggaaccggc gtgaaacgca attcccggcc gctccaggag
     5881 aggcgggcca agtcctggtc gaagcggtgt tggttcaccc agagagacaa ggcgcgcagg
     5941 ttacaatcga tccgggggac gggcgaattt ttccattgac cggccagctc catcccgccg
     6001 aaaaaagtta acggtcccag gtgcacgttc ctcatgtccc cgagcatttg aaaatggccc
     6061 tgcccggcct gtgtcatcaa tatgcggctg ccgtttttca ggcggaagaa cccgtcgggc
     6121 gtttccacgg tgaagtccga aaattgcagt tccttgttct gccactgaac ggtcccctgc
     6181 catttccgca acatgaaatt tccgaaattg ttttcgccgc cggagaacgt tccggagagc
     6241 acggggcggg tcaaggggcc ccgccaagtg aaccggccgt ccaccggcaa tttccagtcg
     6301 ggctgggcgc cgaaaagatg gtggacgggg aaccccgtca aattcacggc cagatcgatc
     6361 tggggcgcgg aacgtttctc ggcatcgaac gacaccgtcc cccttcccgc aagggaggcg
     6421 ggttggcccg atggcccggc gtggatatct tcgagctcca cccgctggcc gtcccagctt
     6481 cctttcatcg agatatcgaa cggcgcccca gtccacgcgg cgtttttcca agtgattcgg
     6541 aaggtgttgc gcggcgcttt ccagggaccc tcccaattca ggacggcgtt gacgcgtccc
     6601 gagcgcagaa gcggatgggc catcccccat cgcggggccc acgccgacat ctcggcttcc
     6661 tgaacgagga gttgcccccg cagggaacgt tgtccggggt tgagggagac ctccgctcgg
     6721 agatcctcgc cgatgcggag ttccgcgagg cggaggtcgg tcccgtccca ttggaatttc
     6781 ccggacgcac ttcccaggcc gatcccgttc acccagaagt tgggcgaacg gaactcgccg
     6841 tggggccgtc cggtgaccgt cgtgtcgccg gcggaaacat gcagctcccc gtccaaggtg
     6901 agggacggaa ggacgaagtg tttgaattgc gccgtgaaag aggaggggac cgtcgttttt
     6961 ttccctaggg gcagggctcc ctccaggtga acggagcccg aaggctggga gacgtcgaac
     7021 cccttcagac tcaaaacacc acccgaaatt tttgcggcgc cacggacctt gtcgaacgga
     7081 agggaacccc atttcccgcg tgtccaggtg acatcggccg tcccctccca gttcgaaact
     7141 cccttttggg agacgttgag atggaaatcc ccgttgatct ttccctccag gggcgcgtcg
     7201 agcaggatca aatcgccgag aaaacgggcg tccccgtcca cggcttttaa tcgggcgtcc
     7261 caaccgtgtc ggtccgccca acgcagttga ccctcccacg ccgagcccac gcgccacggg
     7321 gagagcgcga attcctcgtt tgtccagtgc acatccgtgt gggcatccac ggggtcgctc
     7381 gtgcggcgca gctttaagac gttcaaatct cccgttcccg taaaaaccgg cgcggccgcc
     7441 gttccggaca ggcggccgtc gaaatcaatg gagcctgaaa tttccggata acgttccagc
     7501 agcatgggca ggtctcggac gggcatgcgt ttgacggagg cctgcaggga gagggtccgt
     7561 tccggtcctt ccaccgtgcc gttcgccgag agggtcatgc cgctgggcag ggacaactcc
     7621 agttccgaca ggacggtttt ttcgggagag aaggaggcgt tcagtcggaa aaggttgctg
     7681 aggtcttgga tgaggagctg tccctttttg aacgagagcc gtccggagat gggagctgtt
     7741 tccttgtgga cgccccaggc gaaacggtcc actttgagga agccgtccgc ttccagggtg
     7801 cggagatcga aggcggggcc tttccggagg cgtgctttgc cgctcaccgt gccggagatg
     7861 tttccccacc ggttcagtcg cgcccattcc cccatctcga ggccgttcgc ccgcaaggtc
     7921 cacagggaac ggacttcttc cgtcggtgtg gaggggtccg ggagcggttc ctgggagaaa
     7981 aggagccgcc cattgaggaa agaggtctcc ccctcccacc ttcccgggtg accttggaga
     8041 gagaccgtca gggagggcag cgcgatgccg gcccatttgg catcgcgcaa cgaaacgtgc
     8101 gcggtcgttt catgggccgt cgggggaccg gtcacgcgtg cg
//
LOCUS       DAOTFD010000108         8094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6278_length_8094_cov_4.304018, whole genome shotgun sequence.
ACCESSION   DAOTFD010000108 DAOTFD010000000
VERSION     DAOTFD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6278_length_8094_cov_4.304018"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="P5079_07070"
     CDS             <1..446
                     /locus_tag="P5079_07070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY29784.1"
                     /translation="EYLGHPGAVAVVPVLDDRSADPRLLLVRQFRYPVGELTLELPAG
                     KLDGREAPVRCVRRELEEETGYTARRVWKMLSYWPTPAFANEIIHVFGASGLRRGTYS
                     PDEDEIIEPVVLRLSRVLEMIRRGRLRDSKTIVGLLAFSRWMKKS"
     gene            660..1721
                     /locus_tag="P5079_07075"
     CDS             660..1721
                     /locus_tag="P5079_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRY29785.1"
                     /translation="MSFTGKCGLALFFAMAVSPVFAAVAGDIIINEIMYNDYGSGTDW
                     IEIYNSSTSNIDLTGWKVWDGDGTTWHGLTFTQGGSTISAGGYAIVAENATTFLSGHA
                     ASAGVGSWIVIDSGGLTLGQSGTDTVALSTAGTGSTVISSVTYGVASPWPTGVEGYSI
                     ERKVFAGTDSDPTNWVLGPSSGTPAALNVSGGGSAVTTEISCINGVDDDGDGMVDCAD
                     GDCAADTACVVPGATNSTAKVLEVDDSRNPFSPYDKDSAYRLGRILFNVYAADVIKTI
                     RIVNVRGESVRMLINNDMGPSNASYTGVAGGLVTWDGRDDEGNLVPVGIYIVFLEGVN
                     PGTGDRVTARDTIAVGRPF"
     gene            1837..2220
                     /locus_tag="P5079_07080"
     CDS             1837..2220
                     /locus_tag="P5079_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY29786.1"
                     /translation="MVTQSDGEFDFERLDVYQRAVSFTNDLFRFCNNLPFKVQSSLGD
                     QLRRASLSVCNNIAEGSDKASSKEKIVFYNYALSSMRECVPCMTVAFDQNLLSGEKLN
                     EFREKCLIICRMLKRLIQSVERRGK"
     gene            2217..3848
                     /locus_tag="P5079_07085"
     CDS             2217..3848
                     /locus_tag="P5079_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY29787.1"
                     /translation="MKPIKIPQKYSLALLVSLQLLTFNIKLLHASFEEKPVSARAAGM
                     GEAMAAVSGDANSFFYNPAGFRFSQHKELLTNYTRLFDVPDLPYQNFAVGLPTQKYGA
                     WGLAYGQFGPSEYREQEFLLSHSVLLSPAAAFGYSIRREGLKLKRYGSTAAWGLDLGV
                     VGQLHPKVKGGFAVKNLNHPNYGSSSEGPSQEMRAGVAFKALPGFLTSFDAVKPAEGA
                     LSYRGGEEIQISRVLALRFGVETAPNRFSGGLGLRFKIFHLDYAFLTHPFLHDQQHVT
                     LSLWWGGEEELQVAEAAPKARRAKKKALPKKLMEGSAQKVDINAASAEELQALPGIGG
                     TMAERIVEYRQQKGRFQRDTDLLNVPQFQRRIFIRVRPYLIVGPGDGKSAVPVPEPSP
                     AVVPPPAPEPAPVEEEMPEEEPEQPAEEEEKPAPAAVPAPVPAPASPPVLPSSPPAPE
                     PVPSPAVPVEPAPPAEEVPASEPETRAAPTGGLDVNSASAAQLERIGFSSVQAKNIVR
                     YRQRKGSFSSVNDLRNVPGVSGRTLDAVRGRLSAE"
     gene            3883..6168
                     /locus_tag="P5079_07090"
     CDS             3883..6168
                     /locus_tag="P5079_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY29788.1"
                     /translation="MPPPRRFHSSGFACLWAAGLLAGFSVFSAAEEMESAGEVTDPPA
                     LSEPVRASSAPPVDLPPSLDEDQDSEEGASVDTGQKGDEDPLTELSEHPLDLNKAKID
                     DLIVLPGVDRPMAQNIVRERRRRKRFNAVDELTDVSGIDVAFLERIREYIVVEPLIEK
                     GKRAFFSGDTRARMRNVNPRGSSFFNTPSQFQNNTYVYNRTRMRFGEQSQAGWLAKRP
                     SVGMPLTPENNQRLSLFKFSVQESDAGWFDKVVIGNYTLCHGQGLIFYDGLGEYVRPA
                     KVKARGAKQDFTSGANEYHKGVVVDGRVGFFDLEAFVSQKDLDFEVDSASGVVKADIN
                     SLRQGLGDLQDEEAVVNNDTVTERLYGGRVACALPGNTKVGATAYESHYSKVFRSDRT
                     TYSDAHIFQGNKNVMAGVDFDSFYKSLNLYGEAARSRSSGPGMPHKTGTAWTVTPMLR
                     LTPFVLWSSFFDYDADFFTRHGKGVSFAIVGAPEELTTNQRGVQMGLEYRGKKYTNRT
                     NYYIATFPDAMGNGDNSDPVQASQGRQIYFENLYKVTRPVELYFRWQRTEQDFFPTLE
                     LNGASTGRRRQLLKDVQKFRYQVTWTASSQVRYRVRYETRYEDVPEYPTRAFGHLVMG
                     DVKYKPTRDLTLNARVYFFDSPTASLTTGVEEIWDKTVYYRLAGAMNSLKGDPGTRFY
                     LIAKQRLGRDLSVWMKYDVNYRPGKTRSIERTVSDQESAAFGSTRQGFHMQVDYKWGN
                     SGRLKEDTEYRTLSDEFTSQE"
     gene            6223..7500
                     /locus_tag="P5079_07095"
     CDS             6223..7500
                     /locus_tag="P5079_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29789.1"
                     /translation="MKRRTLLVLTMTAALSGAGRLWGETYLGVREFGDAVCGAGARSV
                     AMGSTGLARARQAAVFRMNPAVLNDLEKREISVTPGPVFVTEKFVEASSETRFRNQEE
                     LQINEVTVALPLKSNRLWFAVGASPAYDFNYEEAYTLYSSTGSPSSDIRLKDSGVLWA
                     VTPAISVGLSDTFSLGVAYDFWTGQEKLGVTRFQYNAAYAVDSHEKADYSGGAARVGF
                     RWKASDKFSFGAMYQPSGKLTRDFDFINSTAPEKNRSGSDTWDMPAQWGFGISYFLEG
                     EHATEAVMEVRQTLWGAVKVDGQKVNKLAATKPIFQETSGYMFSGAGAVPLAPRSYSD
                     VTEIRVGVEHTLTEQWQIRFGFHHLPYFADRTVEATYFTGGAGFQPNESWAWSLAGEF
                     GKRDFKGENLFFDVTRRVDETFRRFLLTGSFRW"
     gene            7678..>8094
                     /locus_tag="P5079_07100"
     CDS             7678..>8094
                     /locus_tag="P5079_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRY29790.1"
                     /translation="MRLSGCFKLLTFNFKLAVVGTALLAFNVKPASAAVVLNEVVYDP
                     SGSDTGLEWIELYNNGSSAVDLAGWTIQLDQNGVWTDTTTSVSATLAGLIAPQSFFLI
                     EDTQDASKQPADLLLPVGDTLGMGNASSKAEGLRLLN"
BASE COUNT         1616 a         2261 c         2576 g         1641 t
ORIGIN      
        1 gcgaatatct tggccatccc ggcgcggtgg cggtggttcc ggtgctggac gaccggtcgg
       61 ccgatccgcg cctgttgctg gtgcgacaat tccgttatcc ggtgggggaa ctcacgctgg
      121 aactgcccgc cgggaaactg gatggccgtg aggcgccggt gcgttgcgtg cggcgcgagt
      181 tggaggagga gaccggctac accgcccggc gcgtgtggaa aatgctttcc tactggccca
      241 cgccggcttt tgccaacgag atcatccacg tcttcggcgc ctccggcctg cgccgcggga
      301 cgtatagtcc cgatgaggac gagatcatcg aaccggtcgt cctgcgcctg agccgggtgt
      361 tggaaatgat ccgccgcggt cgccttcgcg attccaagac catcgtcggc ctgttggctt
      421 tctcgcggtg gatgaaaaag agctgaactt cgggcgaagg gaattgtaaa atgggcttgt
      481 tcagtcgctt gaaaggatat gggatgaatc ggaccaaagg attgcatgct cgggtttttc
      541 aaatcaaaac cgctgaggct ccgctgaaat catgtgggga cgacgacaat gggaccgtca
      601 ttcccgcgcg ttttatgcgg ggattcagaa agggggaggt ccgggggggc cggcgtgtga
      661 tgtccttcac tgggaaatgc gggctggccc tatttttcgc catggcggtt tctccggttt
      721 tcgcggccgt cgccggggac attatcatta atgagatcat gtataacgat tacggttcgg
      781 gaacggactg gatcgaaata tacaacagtt ccacctcgaa tatcgatttg acgggatgga
      841 aagtttggga tggcgacggc accacttggc atggcctgac atttactcag ggaggttcga
      901 ccatttcggc gggcggatac gctattgttg ccgaaaatgc caccactttt ttatcgggcc
      961 atgcggcttc tgccggtgtc gggagttgga tcgtcatcga ttcgggtggt ctgacgttgg
     1021 gtcagtccgg caccgatacg gttgctctca gcacggcggg gacggggtcg accgtgatca
     1081 gttccgttac ttatggggtc gcgtctccct ggccgaccgg ggtggaggga tattcgatcg
     1141 agcggaaggt ttttgccggg acggacagcg atccgaccaa ctgggtgttg ggtccttcca
     1201 gcggcactcc ggcggctttg aatgtttccg ggggcggcag cgccgtgaca acggaaatca
     1261 gctgcatcaa cggggtcgat gacgacggcg atggaatggt ggactgcgcc gacggcgatt
     1321 gcgccgcgga cacggcctgc gttgttccag gggccaccaa ttccaccgcc aaggttttgg
     1381 aagtggacga ttcccgcaac cccttctcgc cctacgacaa agattccgcc taccgcctcg
     1441 gccgcatcct cttcaacgtc tacgccgccg acgtcatcaa gaccatccgt atcgtgaacg
     1501 tccggggcga atccgtccgc atgctcatca acaacgacat gggcccgtcc aacgcctcct
     1561 ataccggcgt ggcggggggg ctggtcacct gggacggccg ggacgacgag gggaacctgg
     1621 tgccggtggg catctatatc gttttcctcg aaggggtcaa tccgggcacg ggagaccgcg
     1681 tcaccgcccg agacaccatc gcggtgggaa gaccgttctg aaaacggttt aaagttgaaa
     1741 gtttaaggtt taatggggaa agacggagga gtcagtttaa aattggaagt gaaaagtcaa
     1801 aagggggaag caagaagaat agcgtaaagg caatgtatgg tcacgcagag cgatggcgaa
     1861 tttgattttg aaagactcga cgtttatcag agagctgtca gtttcactaa cgatcttttc
     1921 cgattctgta ataacctgcc gtttaaagtg caaagttctt tgggggatca attaaggcgc
     1981 gcatcattgt cggtttgcaa taatattgca gaaggcagtg ataaggcgag ttcgaaagaa
     2041 aaaattgtgt tttataatta cgcgctcagc tcgatgagag aatgcgtgcc ttgtatgacg
     2101 gttgcattcg accaaaatct tctttctggc gagaagttga atgaatttcg tgaaaaatgt
     2161 ctgattattt gccgtatgtt aaaacgttta attcaatctg ttgaaaggag ggggaagtga
     2221 agccaattaa aattcctcaa aagtattcac tcgctttgct tgtctccctt caacttttaa
     2281 cctttaacat taaacttctg catgcttctt tcgaggaaaa gcccgtttcc gcccgcgccg
     2341 cgggcatggg cgaggccatg gccgccgttt ccggcgatgc caacagtttt ttctacaacc
     2401 ccgccgggtt ccgtttctcc caacacaagg aattgctgac caactacacc cgccttttcg
     2461 acgtgccgga cctgccttac cagaatttcg ccgtgggcct gccgacccag aaatacgggg
     2521 cgtggggcct ggcctacggc cagttcgggc cgagcgaata ccgcgagcag gagttccttc
     2581 tgtcgcacag cgtccttttg agtcccgccg ccgctttcgg gtattccatc cgccgcgagg
     2641 gactgaaact gaaacggtac ggttccaccg ccgcgtgggg gttggacttg ggcgttgtgg
     2701 gccagcttca tccgaaggtg aagggcggtt ttgccgtcaa gaatttgaac catcccaact
     2761 acgggagttc ctccgagggg ccgtcgcagg agatgcgtgc cggggtggcg ttcaaggcgt
     2821 tgccgggttt cttgacctct ttcgacgcgg tgaaaccggc cgagggggcg ctttcctacc
     2881 gcggcggcga ggagattcag atctcccgcg tgttggcgct tcggttcggt gtggagacgg
     2941 ccccgaaccg tttttccggc gggctgggcc tgcgtttcaa gattttccat ctcgactacg
     3001 ccttcctgac gcaccctttc ctgcacgacc agcagcacgt gacgctgtct ttgtggtggg
     3061 gcggcgaaga ggaactccag gtggcggagg cggctcccaa ggcccgccgg gcgaagaaga
     3121 aagccctgcc gaagaaactc atggaggggt ccgcccagaa ggtggatatc aacgcggctt
     3181 ccgcggagga attgcaggca ttgccgggca tcggcggcac catggcggaa cggatcgtgg
     3241 agtatcgcca gcagaagggg cggtttcaac gggacacgga cctgttgaac gtcccccagt
     3301 tccagcggcg catttttatc cgtgtgcggc cttatcttat cgtcgggccg ggcgatggga
     3361 aatcggcggt cccggtcccc gaaccgtccc cggccgttgt tcctccgccg gccccggagc
     3421 cggccccggt tgaggaggaa atgccggaag aggaaccgga acagccggcg gaagaggaag
     3481 aaaaacccgc tccggcggcc gttcccgcgc cggtgccggc gcccgcctcg ccgcctgtgt
     3541 tgccttcgtc gccgccggcc ccggaacccg tgccgtcccc ggccgtcccc gttgagcccg
     3601 cgccgccggc ggaggaagtt cccgcttccg aaccggagac ccgcgccgct cccacggggg
     3661 gattggatgt caactcggcc tcggccgctc aactggagcg gatcggtttt tcgtccgtgc
     3721 aggcaaaaaa catcgtgcgc tatcgccaac ggaagggttc gttttcgtcg gtgaacgacc
     3781 ttcgcaacgt gccgggggtc tccggccgga ccttggacgc cgtgcgcgga cgcctctccg
     3841 cggagtaaca agagccctgt gcggaaagca ggagacctgt tcttgccccc gccccgccgt
     3901 ttccattcca gcggttttgc gtgtttgtgg gccgccggcc tcctggccgg tttttccgtc
     3961 ttttccgccg ccgaggagat ggagtcggcc ggagaagtga cggacccgcc ggctctttcg
     4021 gaaccggtcc gcgcctcgtc cgccccgccc gtggatctgc cgccttcctt ggacgaagac
     4081 caggacagcg aagaaggggc ctctgtggac accggtcaaa agggggacga ggatcccttg
     4141 acggaactct cggaacatcc cctcgatctg aacaaggcaa agatcgacga cctcatcgtt
     4201 ttgcccgggg tggaccgccc catggcccag aacatcgtgc gggaacggcg ccgccgcaag
     4261 aggttcaatg ccgtggatga actcaccgac gtttccggta tcgacgtggc gtttttggaa
     4321 cggatacggg agtacatcgt ggtggaaccg ctgattgaaa aagggaaacg cgcgtttttc
     4381 tccggcgaca cgcgcgcccg catgcgcaac gtgaacccgc gcggctcgtc tttcttcaac
     4441 accccttcgc agttccagaa caacacctac gtctacaacc gcacccggat gcgcttcggg
     4501 gaacagtccc aggcggggtg gctggccaag cggcccagcg tggggatgcc gctcacgccg
     4561 gaaaacaatc agcggctgtc cctgttcaag ttttccgtcc aggaatccga cgcggggtgg
     4621 ttcgataagg tcgtcatcgg caactacacg ctctgtcacg ggcagggcct cattttttac
     4681 gacgggctcg gcgaatacgt gcggccggcc aaggtgaagg cccgaggcgc caaacaggat
     4741 ttcaccagcg gcgccaacga ataccacaag ggcgtggtgg tggacggccg cgtcgggttt
     4801 ttcgacctgg aggccttcgt ttcccagaag gatctggatt tcgaggtcga ctccgccagc
     4861 ggcgtggtga aggccgacat caactctctg cgccaggggc tcggcgacct gcaggacgag
     4921 gaggccgtcg tgaacaacga caccgtgacg gaacgcctct acggcggccg ggtggcctgc
     4981 gctctccccg gcaacaccaa ggtcggcgcg acagcctacg aatcccatta cagcaaggtg
     5041 ttcaggtccg atcgcaccac ctacagcgac gcgcatatct tccaagggaa caagaatgtc
     5101 atggccggcg tggatttcga ttcgttttat aagagcctca acctgtacgg cgaagccgcc
     5161 cggtcccgct ccagcgggcc cggcatgccg cacaagaccg gcacggcgtg gaccgtcacc
     5221 cccatgctgc ggctgacgcc gttcgttctt tggagcagtt ttttcgacta cgacgcggat
     5281 ttcttcaccc ggcacggcaa gggcgtttct ttcgctatcg tgggggctcc ggaagaactg
     5341 accaccaacc agcggggcgt gcagatggga ctggaatacc gcgggaaaaa atacaccaac
     5401 cgcacgaact actacatcgc cacgttcccc gacgccatgg ggaacggcga caacagcgat
     5461 cccgtccagg cctcccaggg gcggcaaatc tatttcgaga acctctataa ggtgacgcgg
     5521 ccggtcgaac tttacttccg ttggcagcgc acggagcagg atttttttcc gacgttggaa
     5581 cttaacggcg ccagcaccgg ccgccgccgg cagttgctga aagacgtcca gaaattccgc
     5641 tatcaggtca cctggaccgc ctcgtcgcag gtgcggtatc gggtgcgcta tgaaacgcgc
     5701 tatgaggacg tcccggaata cccgacccgg gccttcggcc acctggtgat gggggacgtc
     5761 aagtataagc ccacgcgcga tctgacgctg aacgcccgcg tctatttctt cgattcgccg
     5821 acggccagcc tgaccaccgg cgtggaggag atctgggaca agaccgtgta ttaccgtctc
     5881 gccggcgcca tgaacagttt gaagggcgat ccgggtaccc ggttctattt gatcgccaaa
     5941 cagcggttgg ggcgggactt gtcggtctgg atgaaatacg atgtcaatta ccggcccgga
     6001 aaaacgagga gcatcgaaag gaccgtgtcg gatcaggaaa gcgcggcgtt cggctccacc
     6061 cggcaggggt ttcatatgca ggtggactac aaatggggca actccggccg gttgaaggaa
     6121 gacacggaat accggacgct ttccgacgag ttcacgtccc aggaatgaac gcggagcgga
     6181 tttgaacggt tgtttgcgta aaaaaaaatt cgaggtgcgg ctgtgaaaag acgaacactt
     6241 ttggttttga cgatgacggc ggccctgtcc ggcgcgggcc ggctgtgggg ggagacgtat
     6301 ctgggcgtcc gggagttcgg cgacgccgtt tgcggcgccg gcgcgcgcag cgtggccatg
     6361 gggtccaccg gcctggcccg cgcccgtcag gcggcggtgt tccgcatgaa cccggcggtg
     6421 ttgaacgatc tggagaaacg ggagatctcc gtgacgcccg gcccggtgtt cgtgacggaa
     6481 aaattcgtgg aggcttccag cgagacccgc ttccgcaatc aagaggaact gcagatcaat
     6541 gaagtgacgg tggcgttgcc gttgaaatcg aaccggttgt ggttcgccgt gggggcgtcg
     6601 cccgcgtacg atttcaatta cgaggaagcc tatacgctct attcgagcac cgggtcgcct
     6661 tcctcggaca tccggctgaa ggattcgggg gtgttgtggg cggtgacccc ggcgatttcc
     6721 gtcggccttt ccgacacgtt ctcgttgggg gtcgcctatg atttctggac gggacaggag
     6781 aagctcgggg tgacgaggtt ccagtataac gccgcctacg cggtggattc ccacgaaaaa
     6841 gccgattaca gcggcggggc ggcgcgcgtg gggttccgct ggaaagcctc ggataagttc
     6901 agttttgggg cgatgtatca gccttccgga aaactgacgc gcgatttcga tttcatcaac
     6961 tccaccgccc cggaaaaaaa ccgatcgggg agcgacacgt gggacatgcc ggcgcaatgg
     7021 gggttcggga tttcttattt cctcgaggga gagcacgcca ccgaggcggt gatggaggtg
     7081 cgccagaccc tgtggggggc cgtcaaggtg gacggccaaa aggtgaacaa gcttgccgcc
     7141 acgaaaccga tattccagga gacatccggt tacatgtttt ccggcgcggg cgctgtgccg
     7201 ctcgcgccgc ggagttacag cgacgtgacg gagatccgcg tgggggtgga acatacgctg
     7261 accgagcagt ggcagatccg gttcgggttt catcacctgc cgtatttcgc cgaccgcacc
     7321 gtggaggcca cctacttcac cggcggcgcg gggttccagc cgaacgaaag ctgggcctgg
     7381 tctctcgccg gcgagttcgg caagcgggat ttcaaggggg agaacctgtt tttcgacgtg
     7441 acccgccgcg tggatgagac gttccggcgg ttccttttga cgggaagttt ccggtggtaa
     7501 ccgtagaaaa agttaaaagt gaaaggttga aagtttaaag aaaaaaacgg gttttaaaag
     7561 acggtttgaa aatcaaaagt tgaagttttg ggattgaaaa ggtgaatgat agtggggaaa
     7621 aagttaagag ttgaaaacat aaagggtaaa gaaaaaacgt tcggaggcgt attcgccgtg
     7681 cgcttgtccg gctgctttaa acttttaact tttaacttta agctcgccgt ggtcgggaca
     7741 gcgcttttag cgttcaacgt taaaccggct tccgccgccg tggtgctcaa cgaggtcgta
     7801 tacgacccca gcgggtccga cacggggttg gagtggattg agctttataa caacggttcg
     7861 tcggcggtgg acctggcggg gtggacgatt cagctcgatc agaacggcgt ctggacggac
     7921 acgaccacgt cggtgtcggc gacgttggcc gggctcatcg ccccgcagtc ttttttcctc
     7981 atcgaggaca cgcaggatgc ctccaaacag ccggccgatc ttttgttgcc ggtgggagac
     8041 acgctcggca tgggcaacgc cagctccaag gccgaagggc tgcgtctgtt gaac
//
LOCUS       DAOTFD010000109         8040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6343_length_8040_cov_4.641954, whole genome shotgun sequence.
ACCESSION   DAOTFD010000109 DAOTFD010000000
VERSION     DAOTFD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6343_length_8040_cov_4.641954"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="P5079_07105"
     CDS             <1..1210
                     /locus_tag="P5079_07105"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29791.1"
                     /translation="DGRGGEGPRHPLLPRLLAVVFLLDLGLRLLPTKTFCYRGWEALT
                     VNLEFPKLHFSEGPFKRHKTYSNPRTYGDLSALSNLPDRRHYRNETFVTDEFGYRNPP
                     GLTRQEPVSAVLLGDSFVAGAGNSDEFVLPAALRKKSGVVAYNAAGDLPRSLQDILAP
                     VEAAGLREGWVIYEHLERVEKPFKLEGPRERWKIRFWKKFFPLLGRENGLWARQIVSH
                     RFAVSPVKILSAKFLKAFSDDRFFPNSYAKSTVQKRLVTGDVFLFQPQEIEKFHTDRS
                     ALVAPWKTFFLSLSEELARRNLKLMVLLVPEKYTVYEPLLGRGTAAGEKPLYLDALGR
                     ELKAGGLPTVNLAPLFRQKAAELASKKIYLYWPDDTHWTPLGISLAAGEISKILAPDP
                     AGPAARLERK"
     gene            1232..2758
                     /locus_tag="P5079_07110"
     CDS             1232..2758
                     /locus_tag="P5079_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29792.1"
                     /translation="MADIEELVRRHDQYQFEIKLGYDLNKNIRRDVYKVETFFFLPYN
                     LDVNESTYSKKAFYRDLLLYIRFKTPDFPLSSLTNPDNPRSPLTKIAQKIDDALKSPT
                     AAHWDSLGYEIKLLGCVLKSSLRDHINFIEKQFRRFGPGKEQRLELECAPLLNEYLLR
                     TRTAAQSYRAFREKLKSTAVPAASYATYLFLDEYISLLIEGRTHQLLQFLYRAPTTAC
                     PKGTLEKLTDLIREEVDYRSGMGYPSVAKSEDDNEAFVYRLGILKKFAAGILHLDVRT
                     TEEGYGLQQIALAIGAGIAMVFATSIAFYYQRAYGTLSLSFFVALVISYMFKDRLKAA
                     CQSYLQKILSKNLFDQSTDIYDQFNGEKIGLCRENFHFAKEAHLDPLAVRLRNRDHVT
                     EIENEWRSEKIIHYAKEIVLFSRKLLKNQSRKTALTDILRFNVRNFLLKMDEPKTDLF
                     VLEEGKSVVRQGVHVYNVNVVIKFTSGREVRYERFRLVLTRNGIRRIEPIGSEVVSAG
                     "
     gene            complement(2747..3607)
                     /locus_tag="P5079_07115"
     CDS             complement(2747..3607)
                     /locus_tag="P5079_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY29793.1"
                     /translation="MNPFSLPFMQTALAASLLAGTTLSLLGTFILARRQVFSGLAVSQ
                     LAALGTVVGVLVGLHYGASLLALAFVGAGMFFIARFSKIREAPSETWVAGLYVLGAGL
                     AVMVLSKAPRGESETMGVFFGNILTLTNREIWESLVMTVLSAGILWVWFPRWVWLSFD
                     PISAEVSGLRVDGWNLLFYSLFAVVMTFSIHILGVLLAFAYLFLPAAAGFLGVRRMRT
                     LFVFVPSAAVGLTVAGFWLSFRWDFPTGPFLAVLMAIFVVLMRVEASLIRKKSRPTAV
                     LDGGKEGSSG"
     gene            complement(3604..4356)
                     /locus_tag="P5079_07120"
     CDS             complement(3604..4356)
                     /locus_tag="P5079_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY29794.1"
                     /translation="MNESAHIRFEGADLGYNKTAVLKDVTCRIGQGEVIGMVGPNGSG
                     KTTFLRTLLGLVPPLGGTVKVDRSRRFAYVPQLEEVNLFWPLTVRETVCLAQRSKRAF
                     GRVSEEECARVDNAMRKVGISPIADMLFRETSGGQRQKTMLAQALSQEPDVLLLDEPT
                     RGLDVVAERDFLKIVEGLRSKKLTLFIVSHTLHIPLNYTEKILLFHAGRVVEATPDEL
                     LSTKVLDTIYGVPFFHHNDSGIRWAGPKRGGE"
     gene            complement(4432..5358)
                     /locus_tag="P5079_07125"
     CDS             complement(4432..5358)
                     /locus_tag="P5079_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRY29795.1"
                     /translation="MKNWMGLLLGAVLAAPGSLLAAKPLNVFTTTADLADVVRAVGGE
                     RVRVESLSKGYQDAHFVEAKPSLIVKLMSADLFIQTGLELEAGWATVLVQGSRNSRIQ
                     LGAPGYLDASSAVKPLEVPQNPDRAMGDVHPGGNPHYMLDPENVRLVAREIAKRLSSL
                     SPADSAFFRERLGNFEVRLEENLRKWKERMEPFRGARFVSYHRNLPYFADRFGLISVG
                     EVEPKPGIPPTAAHTADLIGRMKAEKVGLIITQPYYEDRSPASIAKQTGATVVVFSMA
                     PSGEPGGSDYFSNMDANIESVRRALAAAAGTK"
     gene            complement(5456..6727)
                     /locus_tag="P5079_07130"
     CDS             complement(5456..6727)
                     /locus_tag="P5079_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29796.1"
                     /translation="MSHRGLSTFVISVVLFLGNSFVSAQENASWMRTQTLSNALNPNI
                     SVIGDFVGTAGPRESEGSNRFALREAELGFQANVDPYARADVFAGWHEGEALELEEAY
                     VTLLALPLALQARGGKFRANFGRLNMIHPHELPQVDQPLVLERYLGGDGLNSAGFEVS
                     RVFGPFGLFTEFSYAVLNDLGGAHEHGEEAGQTVFVNARDGNGNLIVDGNGTPVEIPV
                     TVREEEEPVKRSVRNFAHVGRVRFYRDMTDTANVELGLSGVLHQPEGHEQRRMAGADV
                     TFRWKPLRRGLYRSFLWRTEGVYSTRKLEAVSDLSGAVTAPEMEVDRRGFYSYVELQP
                     ARRWRFGLRGDYVEDPEAKDEVLRKADGTTFQVSRSITRAISPVVTFTLSEFNRFRAQ
                     YQCKRLPDRETENIGYLQWTTILGPHGAHPF"
     gene            complement(6811..7074)
                     /locus_tag="P5079_07135"
     CDS             complement(6811..7074)
                     /locus_tag="P5079_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29797.1"
                     /translation="MKRFLSAALVAAFVLSFPLHSRMHASDTDRGPHVCRVCSLGNAQ
                     HAAPAPSDFGVRLVPLHALEIGEAVGLSPESTCRAFPRAPPPC"
     gene            complement(7141..7749)
                     /locus_tag="P5079_07140"
     CDS             complement(7141..7749)
                     /locus_tag="P5079_07140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY29798.1"
                     /translation="MKRSWFFSWALALLVLPVAEDLSAGGTWSKEPGIYAVFETSQGK
                     IVCKLMPDKAPKTVDNLVGLAEGTKQFTDMKSNKLVKRPFYDGLIFHRVIPNFMIQGG
                     DPLGVGSGGPGYRFEDEIAPGLGFDKPGRLAMANAGPGTNGSQFFITVAPTPWLNGRH
                     TIFGQVVEGQSVADAIANVPRDGQDRPQTPVTIKKLKIVRVK"
     gene            complement(7781..>8040)
                     /locus_tag="P5079_07145"
     CDS             complement(7781..>8040)
                     /locus_tag="P5079_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY29799.1"
                     /translation="AGKKGEELYRADCAMCHEHHKMGLRRIGPSAEEMAGKPVKVLQS
                     VIENGAEGSSMPAFHRDVGGPLSDKDVESLVKFIRSVREEK"
BASE COUNT         1746 a         2695 c         2175 g         1424 t
ORIGIN      
        1 tgatggacga ggtggagaag gtcctcggca tcccctgctc ccccgcctcc tggcggtggt
       61 cttccttctg gacctgggcc ttcggctcct ccccacaaaa acgttctgct accgcgggtg
      121 ggaagcgctc accgtcaacc tggagtttcc aaaactgcat ttttcggaag gaccgttcaa
      181 acgccacaaa acctattcca atccccgcac ctacggggac ctctccgcgc tctccaacct
      241 gccggaccgg cggcactacc ggaacgaaac cttcgtcacc gacgaattcg gatatcggaa
      301 cccgccggga ctcacccggc aggaaccggt ctccgccgtg ctcctcgggg actcgttcgt
      361 ggccggcgcg gggaattcgg acgaattcgt gctgcccgcg gccctgcgga aaaaatccgg
      421 cgtcgtggct tacaacgccg ccggggatct gccccgctcc ctgcaggaca tcctcgcccc
      481 ggtcgaggcc gccgggttgc gggaaggctg ggtgatctac gaacacctgg aacgcgtgga
      541 gaaaccgttc aaactcgaag ggccccgcga acgctggaaa atccgctttt ggaaaaaatt
      601 cttcccgctc ctcggccggg agaacgggct ctgggcccgg cagatcgtct cgcaccggtt
      661 cgccgtctcc ccggtgaaga ttctgtcggc caaattcctc aaggccttct ccgacgaccg
      721 gtttttcccg aactcctacg ccaagtccac cgtacagaaa cgcctggtca ccggcgacgt
      781 tttcctgttc cagccgcagg agatcgaaaa attccacacc gaccggtcgg ccctcgtcgc
      841 cccgtggaaa accttcttcc tttcgctctc ggaggaactg gcccggcgaa acctgaaatt
      901 gatggtcctg ctggtgccgg aaaaatacac ggtctacgaa ccgttgctcg gccgggggac
      961 cgccgccggc gaaaaacccc tctatctcga cgcgttggga cgggaactta aagccggcgg
     1021 actgccgacg gtcaacctcg cgccgctgtt ccgccaaaaa gccgcggagc tggcctcgaa
     1081 aaagatctat ctctactggc ccgacgacac ccactggacc cccctgggca tctctctcgc
     1141 cgccggggag atatcaaaaa tactcgcccc cgaccccgcc ggaccggccg cgcggcttga
     1201 aaggaaataa ggaaaggcat agaatctgcg catggcagac atcgaggaat tggttcgccg
     1261 acacgatcaa taccaatttg aaataaaact cggatacgat ctcaacaaga acatccgccg
     1321 ggacgtttac aaggtagaaa ccttcttttt ccttccctac aacctcgacg tcaacgagtc
     1381 cacctactcc aaaaaagcct tctaccggga cctgctcctc tatatccgct tcaagacgcc
     1441 cgacttcccg ctgagttcgc tcaccaaccc agacaatccc cgttctccgc tgacaaagat
     1501 cgcccaaaag atcgacgatg ccctgaaatc gcccaccgcg gcgcactggg acagcctcgg
     1561 gtacgaaata aaactactgg gttgcgtcct gaaaagctcc cttagggacc acatcaactt
     1621 catcgaaaaa caattccgcc gtttcggccc cggcaaggaa cagcgcctgg aacttgagtg
     1681 cgcccccttg ctgaacgaat atctcctccg gacccgcacc gccgcgcaat cctaccgcgc
     1741 tttccgggaa aaactcaaat ccaccgccgt cccggccgcc tcctacgcca cctacctctt
     1801 cctcgacgaa tacatcagcc tgctcatcga aggacggacg caccaacttc tgcagttcct
     1861 ctaccgggcg ccgaccacgg catgccccaa gggaaccctg gaaaaattga cggacctgat
     1921 acgggaggag gtggactacc gaagtgggat gggctatccg tccgtggcca aaagcgaaga
     1981 cgataatgag gcgttcgttt accggctggg gatacttaag aaattcgcgg ccggcatcct
     2041 gcacctggac gtgcgcacca cggaggaggg gtacggcctg caacagatcg cgctggccat
     2101 cggcgccggt atcgccatgg tcttcgccac gtccatcgcc ttctactacc agcgggctta
     2161 cggtaccctg tccctctcct tcttcgtggc gctggtgatc agctacatgt tcaaggacag
     2221 gctcaaggcc gcctgccaga gctatctgca gaaaatactt tcaaaaaacc tattcgacca
     2281 gtccaccgac atctacgacc agtttaacgg agaaaaaatc ggcctttgcc gggaaaactt
     2341 ccacttcgcc aaagaagcgc atctggaccc cctggcggtc cggctccgca accgcgacca
     2401 cgtcacggag atcgagaacg aatggcggag tgaaaaaatc atccactacg cgaaggagat
     2461 cgtcctcttt tcgcggaagc tgctgaaaaa tcaatcccgc aaaaccgccc tcaccgacat
     2521 cctccggttc aacgtgcgaa acttcctgct gaagatggac gagccaaaaa cggacctctt
     2581 cgtccttgag gagggaaaga gcgtcgtccg gcagggcgtt cacgtgtaca acgtcaacgt
     2641 ggtcatcaag ttcacctccg gcagggaagt ccgctacgag cgcttccgcc tggtgctcac
     2701 gcgcaacggc atcaggcgga tcgaaccgat cggctcagag gtcgtatcag ccggatgatc
     2761 cttccttccc gccgtctagg accgccgtcg gacgcgattt ttttctgatc aaactcgcct
     2821 ccacccgcat caacaccaca aaaatcgcca tcaggaccgc caaaaatggt ccggtgggga
     2881 agtcccaccg gaacgacagc caaaaccccg ccaccgtcag tcccaccgcg gccgatggca
     2941 cgaagacgaa caacgtccgc atccggcgca cgccgaggaa tccggcggcg gccggcaaaa
     3001 acagataggc gaaggccagc agaacgccga ggatgtggat ggaaaacgtc atcacaacgg
     3061 caaaaagcga atagaaaaga aggttccatc cgtccacgcg cagccccgac acctcggcgg
     3121 aaatcggatc gaaggaaagc catacccagc gggggaacca aacccacaaa atccccgcgg
     3181 acaggaccgt catgaccagg gactcccata tttcccgatt cgtcagcgtc aggatgtttc
     3241 cgaaaaaaac gcccatggtc tccgactccc cgcgaggcgc cttggacaag accatcacgg
     3301 ccagccccgc gcccaaaacg tagagtccgg cgacccacgt ttccgacggc gcctcgcgaa
     3361 tcttcgagaa ccgcgcgatg aagaacatcc ccgctcccac gaacgccagc gccaagagcg
     3421 acgccccgta gtgcagaccg accaagaccc ccaccaccgt ccccagggcc gccagctgcg
     3481 aaaccgccaa ccccgaaaac acctgccgcc gggccaggat aaacgtcccc aacagagaaa
     3541 gcgtggtgcc cgccaacagg ctggccgcca acgccgtctg catgaacgga agggagaacg
     3601 ggttcattcg ccccccctct tcggcccggc ccagcggatg ccgctgtcgt tgtggtggaa
     3661 aaacggcaca ccgtatatcg tatccaggac cttcgttgaa agcagttcgt ccggcgtggc
     3721 ctccaccacg cggccggcat gaaacaagag gattttttcc gtgtagttca gcggaatatg
     3781 caacgtgtgg ctcacgatga aaagcgtgag cttcttcgaa cggagacctt ccacgatctt
     3841 caggaagtcc cgctccgcca ccacgtcgag tccccgggtc ggttcgtcca acaaaagcac
     3901 gtccggttcc tgggaaagcg cctgggccaa catggtcttt tgtctttgac cgccggaagt
     3961 ctcccggaaa agcatgtcgg cgatgggaga gatgccgact ttccgcatgg cgttatccac
     4021 ccgggcgcat tcctcctcgc tcacgcgacc gaaagcccgc ttcgaccgct gcgccagaca
     4081 gacggtctcc cgcacggtca gcggccaaaa caaattgacc tcctccaatt gggggacgta
     4141 ggcgaaacga cgggaacggt ccaccttcac cgtcccgccc aatggcggga ccaacccgag
     4201 aagcgtccgg agaaaagtgg tcttccccga tccgttcggt ccgaccattc cgattacctc
     4261 cccctgtccg atacgacagg taacgtcttt gaggaccgcg gtcttgttgt aaccgagatc
     4321 ggctccctca aatcggatat gggctgattc gttcacaggc aacgacctcc tcgatttctt
     4381 tcggaaaccc ctcgtttcaa acgcggagag gcggcgtccg cagagacaag tctacttcgt
     4441 tccggcggca gccgccagcg cccggcgcac cgactcgatg ttggcatcca tgttcgagaa
     4501 gtaatcggac cctcccggct cgccggacgg cgccatggaa aaaacaacca ccgttgcccc
     4561 cgtttgtttg gcgatggaag ccggcgaacg atcctcgtaa tacggctggg tgatgatcag
     4621 ccccaccttt tccgccttca tccggccgat caagtccgcc gtgtgcgccg cggtcggcgg
     4681 gatgcccggt ttgggctcga cctcccccac cgaaatcagt ccgaatcggt cggcgaaata
     4741 cgggagattt ctatgatagc tgacgaaccg ggccccgcgg aacggctcca ttcgctcttt
     4801 ccacttccgg aggttttcct ccagacggac ctcgaaattg ccgagacgtt cccggaaaaa
     4861 cgcggaatcg gccggcgaca agctgcttaa tcttttcgcg atttcccggg cgaccaatcg
     4921 cacgttttcc ggatccagca tgtaatgcgg gttgccgccg ggatggacgt cccccatggc
     4981 gcggtccggg ttctgcggca cttccagggg cttgaccgcg gaggatgcat ccagatagcc
     5041 cggcgcgccc aactgtatcc gcgagttacg cgatccctgg accagcaccg tcgcccaccc
     5101 cgcctccaac tccaacccgg tctgaatgaa caggtccgcg gacatgagct tgacgatcag
     5161 agacggtttc gcttccacga aatgcgcgtc ctggtaccct ttggaaagac tttccacccg
     5221 cacccgttcc ccgccgaccg cgcgcaccac atcggccaga tccgccgtgg tggtgaagac
     5281 gttgagcggt ttggcggcca agagcgagcc cggcgcggcc aaaaccgccc ccaacaacaa
     5341 tcccatccag tttttcataa agattctccg tttttaaaat tgtgccggtc ccccgcccgc
     5401 cgttcgaggg acgggaagcc ggcgcctcag tccaattgaa acgtcgccct gtcgattaga
     5461 acggatgcgc gccgtgagga ccaaggatcg tggtccattg caagtaaccg atgttctccg
     5521 tctcccggtc gggaagacgt ttgcattgat actgcgcccg gaaccggtta aactcgctca
     5581 gggtgaacgt caccaccggc gaaatcgccc gcgtgatgga acgggaaacc tgaaaggtcg
     5641 tcccgtcggc cttgcggagg acctcatcct tcgcctcggg atcctccaca tagtcgcccc
     5701 tcaagccgaa ccgccaacgc cgcgccggct gcagttccac ataggaataa aaaccacgcc
     5761 ggtccacttc catctccgga gccgtcaccg cgccggacag gtccgacacc gcctccagct
     5821 tacgggtgga ataaacgcct tccgtcctcc acaagaaaga ccggtaaagc ccccgccgaa
     5881 gcggcttcca tcggaaggtc acgtccgcgc cggccatacg gcgctgctca tgcccctccg
     5941 gctggtgcag cacccccgac aagcccaatt ccacgttggc ggtgtccgtc atgtcccgat
     6001 agaaacgcac gcgccccacg tgcgcaaaat tgcgcacgga gcgtttaacc ggctcctctt
     6061 cttcacgcac cgtcacgggg atttccaccg gggtcccgtt tccgtccacg atgaggttac
     6121 cgttcccgtc gcgggcgttc acgaaaacgg tttgacccgc ctcctccccg tgctcatggg
     6181 ccccacccaa atcgttgagc acggcgtaag aaaactcggt aaaaagaccg aaaggtccga
     6241 acacgcgact gacctcgaag ccggcgctgt tgagcccgtc cccgcccaaa taacgttcca
     6301 acaccagggg ctggtccacc tgcggcagtt cgtgcgggtg aatcatgttg aggcggccga
     6361 agttggcccg gaatttcccg ccgcgcgcct gcaacgccaa cggcagggcc aacaacgtca
     6421 cgtaggcctc ctccaattcc aaagcctccc cctcgtgcca accggcaaag acgtccgccc
     6481 gggcgtaagg gtccacgttc gcttgaaaac ccagctccgc ctcgcgcagg gcgaaccggt
     6541 tgctgccttc ggactcccgg gggccggcgg ttccgacgaa gtcgccgatg acgctgatgt
     6601 tgggattgag cgcgttggac aacgtctgcg tgcgcatcca ggacgcgttc tcctgggccg
     6661 aaacgaaaga attccccaaa aacagaacaa ccgaaataac gaacgtcgat aatccgcgat
     6721 gggacatggc acacctcctg tgaaatggat gacgagcggc gatcggcccg ctcaggaata
     6781 atgatcagat gccgggattt tcggaaagga tcagcaggga ggaggtgcgc gcggaaacgc
     6841 ccggcacgtc gattccgggg agagaccgac ggcctctcct atttccaacg catggagcgg
     6901 aacaagacgg acgccgaaat ccgatggggc cggcgcggcg tgttgcgcgt tccccaggct
     6961 gcaaacacga cagacatggg ggccccgatc cgtatcggac gcgtgcatcc ggctgtggag
     7021 cggaaaagag aggacgaacg ccgccaccag cgcggcggag agaaaacgtt tcatgtcacc
     7081 tcatcatggc acattttaaa cgccgccgtc aaaaccgccg gacggccggg gacgacggat
     7141 ttatttcacc cgcacgattt taagtttttt gatggtcacg ggcgtctgcg gacggtcctg
     7201 cccgtcgcgc ggcacgttgg cgatggcgtc cgccacggac tggccttcca ccacctggcc
     7261 gaagatggtg tggcgtccgt tcagccacgg cgtgggcgcc acggtgatga aaaactggga
     7321 accgttggtc cccggcccgg cgttggccat ggccagccgc cccggcttgt cgaaacccag
     7381 ccccggcgcg atctcgtctt cgaaccggta ccccggcccg ccgctcccca ctccgagagg
     7441 gtctcctccc tggatcatga aattgggaat gacgcggtgg aagatgagcc cgtcgtagaa
     7501 aggccgcttc accagcttat tgctcttcat gtcggtgaac tgtttggtgc cctccgccag
     7561 gccgaccaga ttgtcgacgg tcttgggggc cttgtccggc atgagtttac agacgatctt
     7621 cccctggctc gtctcgaaca cggcgtaaat gcccggctcc ttggaccacg tgccgccggc
     7681 cgacaggtcc tccgccaccg gcaaaaccaa caacgccaaa gcccagctga aaaaccaaga
     7741 acgtttcata ttcgtctcct tgattcgatt tgtgaaaatc ctatttttct tcccgaacgg
     7801 accggatgaa cttcaccagc gactcgacgt ctttgtcgct caacggaccg cccacgtccc
     7861 gatggaacgc cggcatggag gaaccttcgg cgccgttctc aatgacggat tgcaaaacct
     7921 tgaccggttt ccccgccatc tcttcggccg aaggaccgat gcgacgcagc cccattttat
     7981 gatgttcatg gcacatggca caatcggccc ggtaaagttc ctcccctttc ttgcccgccc
//
LOCUS       DAOTFD010000110         7956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6438_length_7956_cov_3.938868, whole genome shotgun sequence.
ACCESSION   DAOTFD010000110 DAOTFD010000000
VERSION     DAOTFD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6438_length_7956_cov_3.938868"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="P5079_07150"
     CDS             complement(<1..184)
                     /locus_tag="P5079_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY29800.1"
                     /translation="MKKTTEKPSLRISLAQINCAVGDLAGNAARILEYAEWAGRQEAD
                     VVVFPELSLTGYPPEDL"
     gene            complement(211..1443)
                     /locus_tag="P5079_07155"
     CDS             complement(211..1443)
                     /locus_tag="P5079_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY29801.1"
                     /translation="MPRPKAALPALDPSEELELLHQITHIINSPLDLKVILQETVSLV
                     SRLTKADACFLYLHDPSESNLVLSASKTPHPGEVGTLRLRLGEGLTGWVAQHRVPLAI
                     AEKAHEDPRFKFFHDLPEDAYEAFLSVPILIREKVIGVINVQHRKPHLYPERTIKVLT
                     TISRQVSGAIENARLYEETKRKAQAIQTLSAVSHTVSSPKYLQETLQLIVTMTASLLG
                     SKICSVMLLDEETQELKIAATQSLSDAYRNKPPIKVGQSVSGQATIQKVPIAVRDVRK
                     DRRFGFPEIAAAEGLVSLLSVPMLFKDKVIGVINTYSAEEYVFSKEDVSVLQSVANQC
                     ASAIEHTRLLTEKLAAQEALESRKLVERAKSALMRKRGVSEPEAFREIQKQSMDRRKS
                     MKEIAEAILLAEELEGKR"
     gene            1752..1949
                     /locus_tag="P5079_07160"
     CDS             1752..1949
                     /locus_tag="P5079_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRY29802.1"
                     /translation="MKGKVKWFNASKGYGFITQESGEDVFVHYSSIQSEGYKSLNEGQ
                     AVEFEATKGPKGLQATNVRAI"
     gene            2305..3945
                     /locus_tag="P5079_07165"
     CDS             2305..3945
                     /locus_tag="P5079_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29803.1"
                     /translation="MMAIWDDGQRLVAFLAVSFIVSAGLPVGAEERMQVRLEKASGES
                     FEPLRTEEVCGAYFPSYYDWRKAALKNNLNVVSKMTATKTAVVVITDAPSPPASEEMF
                     VFEARKAAAAAGANLLCVKPGGRAAEGKMKFDLYRLQFEGKPVPADFIRLLAATSFHD
                     DSYLQSLLEEWIQKTRTVDMNPPPRPSPVVPTLPAAVASPVVPVPPPAKAEDKKEPAA
                     SAVSLPLPVEQVPPPAKEKGEKEAPVGKKEGAGSADKKSSSGGEKKEERKGEPGGPLP
                     TILPGSPVPEMSLSKEDLPLPSADPGRTEQGKVAVSTASPEPRPVEEVPGASSSAPVP
                     SAAVPPSDPAARKTEAPRLKAVGAGCLSMLVPGLGQRAAGDKAKAAVVFATQVGLLTG
                     SAMLGDHIKKNVDRGLSDVYWYRVSKYVAAAYWVSQSAATTVRLWPKKEKTPPDVNGA
                     LARSFVLPGWGLIYSGYYETGFNVMALEGFVLYWGTKSELPKKERLNLVAFSHWGQVV
                     LTGVLAKYKAAGLRAALVPADDGAGVVFVLQKRFGREE"
     gene            3942..4346
                     /locus_tag="P5079_07170"
     CDS             3942..4346
                     /locus_tag="P5079_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRY29804.1"
                     /translation="MKLNELFDLSPGKVEDLRQRVARLGINLAEVEEEFTRGGGKGGQ
                     KINKTANRVVLHYRPLQIVVRCQRERRRSLNRFLALRELIDHIEMRVSPRTSQRLKEH
                     ERRRRQKDRRRRRGERAGDKAGLPETGEGISG"
     gene            complement(4343..5827)
                     /locus_tag="P5079_07175"
     CDS             complement(4343..5827)
                     /locus_tag="P5079_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRY29805.1"
                     /translation="MRKPTGFLTFNRELPHDASPRERLRHWNEFHEPMPEEKLRKQGA
                     RCMDCGVPFCHTGMLLNGMTTGCPLNNLIPEWNDLVFRGLWKDALDRLHKTNNFPEFT
                     GRVCPAPCEGSCVLGINEPPVTIKNIESSIIEEGFDAGWVVAEPPSLRTGKRVAVVGS
                     GPAGLACAAQLNRAGHWVTVFERADRIGGLLMYGIPNMKLDKRTVTRRVDLLTAEGVN
                     FLTNTEVGKNYAAEKLLKEFDAVVLCTGATRPRDLPVPGRRLDGIHYAVEFLHANTKS
                     LLDSGLRDGNYISAKGKDVVVIGGGDTGTDCVGTALRHGCRSVVQFEILPQPPDRRPP
                     DNPWPQWPKILKTDYGQEEAAALQGSDPRNYLIQTKEFLGDPGGRVKEVRTVRVEWKK
                     DEKGRFSPSEIPGSEKIRPAQLVLLAMGFLGPEDGLLKQLGVPQDERSNIRADDGRFH
                     TGVPKIFAAGDARRGQSLVVWAIHEGRAAARECDRFLMGRTQLP"
     gene            complement(5896..>7956)
                     /locus_tag="P5079_07180"
     CDS             complement(5896..>7956)
                     /locus_tag="P5079_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRY29806.1"
                     /translation="NRTKTLCTLRGLLDLTPSTQPVPLEEVESVEDICKRFKTGAMSY
                     GSISQEAHEALAVAMNRIAGKSNTGEGGEDPARFVPDPNGDSRKSAIKQVASGRFGVT
                     SLYLVNAQEIQIKMAQGAKPGEGGQLPGHKVFPWIAKTRHSTPGVGLISPPPHHDIYS
                     IEDLAELIHDLKNANPRARISVKLVSEVGVGTIAAGVAKAHADVVLISGYDGGTGASP
                     QSSIKHAGLPWELGLAETHQTLLLNNLRSRVVVETDGQLKTGRDVVVAALLGAEEFGF
                     ATAPLVVLGCVMMRVCHQNTCPVGVATQDPQLRKKFAGDPAHVVNFMRFIASEVREYM
                     AQLGFRTVNEMVGRADRLAMRRAVDHWKAKGLDYSQILYQPPVPPEVGRYCQTAQDHG
                     LDKSLDRQVLLGLCAPALERREPVTASLPVQNKNRVVGTLVGSELTRRYGAQGLPDDT
                     IRVHFTGTAGQSFGAFLPRGMTFLVEGDTNDYLGKGLSGGKIIVFPPKDSTFAPEETV
                     IIGNVAFYGATGGEAYIRGVAGERFCVRNSGARAVVEGVGDHACEYMTGGHVVVLGRT
                     GRNFAAGMSGGVAYVLDDTGDFPLRCNQRMVDLEPLDAEDIKEVEAMVRRHAEWTKSA
                     LAWRLLADWEKTAAKFIKVMPKDYKRMITAIKRVHASGLAGEEAVMAAFEANARDVSR
                     VGGN"
BASE COUNT         1343 a         2369 c         2635 g         1609 t
ORIGIN      
        1 cgaggtcttc cggcgggtac ccggtgagag agagttcagg aaacacgacc acgtcggcct
       61 cctgccgccc cgcccattcg gcgtattcca ggatgcgggc ggcgttcccg gcgaggtcgc
      121 cgacggcgca gttgatctgc gccagggaga tccgaaggga cggtttttcc gtcgtttttt
      181 tcatgggatt tcggcggggc ggccccggaa ttacctcttg ccttccagtt cttccgccag
      241 caggatcgcc tcggcgatct ctttcatgct cttgcgccgg tccatgctct gtttctggat
      301 ctcccggaac gcttccggtt cgctgacccc gcgtttccgc atgagggcgc ttttggcgcg
      361 ctccaccagt ttgcgggact ccagggcttc ctgggcggcc agtttttccg tgagcagtcg
      421 cgtgtgttcg atggccgagg cgcattggtt ggccaccgat tgcaggacgg acacgtcctc
      481 cttgctgaaa acgtattctt ccgccgagta ggtgttgatc acgccgatga ccttgtcctt
      541 gaaaagcatg ggcaccgaga ggagcgaaac caacccctcg gcggcggcga tctccgggaa
      601 accgaaacgc cggtccttcc ggacgtcccg gacggcgatg gggactttct ggatcgtggc
      661 ctgtccggag acgctctggc ccaccttgat gggcggcttg ttgcggtagg cgtccgagag
      721 ggactgcgtg gccgcgatct tgagttcctg ggtctcctcg tcgagcaaca tcacggagca
      781 gattttcgag cccaggaggg aggccgtcat ggtgacgatg agttggaggg tttcctgcag
      841 atacttgggg gaggagaccg tgtggctcac cgccgacaag gtctggatcg cctgggcctt
      901 gcgtttcgtc tcctcataga gccgggcgtt ctcgatggcg ccgctcacct ggcgggagat
      961 ggtggtgagc accttgatgg tccgctcggg atagaggtgc ggtttacgat gctgcacgtt
     1021 gatgaccccg atgacctttt cccggataag gatcgggacg gagaggaagg cctcgtaggc
     1081 atcctcgggc aagtcatgga aaaatttgaa gcgggggtcc tcgtgcgctt tttcggcgat
     1141 ggccaggggg acccggtgct gggccaccca gccggtgagc ccttcgccga gccggaggcg
     1201 gagcgttccg acctcccccg ggtggggcgt tttggaggcg gaaaggacca ggttcgattc
     1261 gctcggatcg tgcaggtaga ggaaacaggc gtccgctttg gtcaggcggc tcaccaggga
     1321 gacggtctcc tggaggatga cctttaaatc gagaggcgaa ttgatgatgt gcgtgatctg
     1381 gtggaggagc tccagttcct cggacggatc cagcgcgggg agggctgctt tggggcgggg
     1441 catggggcgc aagaactcct ttgtgaggga tgtgattctt tgccgatgac cttccgacga
     1501 cactaattta tcaacttttg agacggaggt caaaatcttc ttcgtcggga aaagaaccct
     1561 tgactattcc gtcggatatg tcataatgac ggtgtaagat tggagtttgg cttcaacgtt
     1621 atcggtgtaa tgagtcttgc atgaaaccgc gtgggcctac atcccaagcg gtctttttta
     1681 tgcggtcggc attagctgtt gcgggagatc cagcttcaaa atctgtctta ctgtaggatg
     1741 gtgagtttca catgaagggt aaagtgaagt ggttcaacgc ttccaagggt tatggtttca
     1801 tcacgcagga gagcggcgaa gacgtgttcg tgcactattc gtccatccag tcggagggct
     1861 ataaatcctt gaacgaagga caggctgtcg aattcgaggc caccaagggt ccgaaagggc
     1921 ttcaggcgac caacgttcgc gcaatctgat ccgttcgtaa aacgattggg gggtccccgt
     1981 caaagaaatt tgacggggac ctttcgtttt tagtcggaaa tgttaagctt taagcgcttg
     2041 gcggggcctc cggccccgaa ccccggccgg actttctttg cgcgtccaaa gatagcccgg
     2101 gcaaagaaac gacgcccccg cggccttccc tcttcctttt tccggcgacc gcgaacttgc
     2161 ctcccccagc ggcctatcgg gaggttcgga catcgcgtct ccccatcccc ggaaaaagtc
     2221 atcggttaaa gaggcctcca ggggggaaca agagagccaa ggaacttgaa taataacggc
     2281 gacacgaaat ttcggaaatc ttggatgatg gcgatttggg acgatggcca acggctggtc
     2341 gcgttcctcg ccgtctcttt tatcgtttct gccggcctgc cggtcggtgc ggaagaacgt
     2401 atgcaggtcc gcctggaaaa ggcttcgggc gagtccttcg agcctctccg aacggaggag
     2461 gtctgcgggg cgtattttcc ttcctactac gattggcgca aggccgcgct gaaaaacaac
     2521 ctcaacgtcg tttcgaagat gaccgcgaca aaaacggccg tcgtcgtcat cacggacgcc
     2581 ccctccccgc cggcgtccga ggaaatgttc gttttcgagg cccgtaaagc cgccgcggcg
     2641 gccggagcca acctgttgtg cgtcaagccg ggcggtcgag ccgccgaggg gaagatgaaa
     2701 tttgatctct accggcttca gttcgagggg aaaccggtcc cggcggattt tatccggctg
     2761 ttggccgcca cctctttcca cgacgactcc tatcttcagt cgttgctgga ggaatggatc
     2821 caaaagacgc ggaccgtgga catgaacccg ccgccgcgtc cctccccggt cgtccctact
     2881 ttgccggccg cggtggcctc gcctgtggtg ccggtcccgc cccccgcgaa ggccgaagac
     2941 aaaaaagagc cggccgcctc ggcggtctct cttcccttgc ccgtcgagca ggttcctcct
     3001 ccggcaaagg agaaggggga gaaagaggct ccggtcggaa aaaaagaggg ggcgggctcg
     3061 gcggataaaa agagttcttc cggcggagag aaaaaggagg aaagaaaggg ggagccgggc
     3121 ggtccgttgc cgacgatttt gccgggttcc ccggtcccgg agatgtctct ttccaaagag
     3181 gatcttccct tgccttccgc cgatcccggt cggacggaac agggaaaggt ggcggtgtcc
     3241 accgcttccc ccgaaccccg accggtggaa gaggtcccgg gggcgtcctc gtcggcgccc
     3301 gtcccctcgg ccgctgttcc gccgtcggat ccggccgccc ggaagaccga ggcgcctcgt
     3361 ctcaaggcgg tgggagccgg atgtctgtcc atgttggtgc cgggattggg ccagcgggcg
     3421 gcgggagaca aggccaaagc cgcggtcgtc tttgcgacgc aggtgggcct tctgaccggc
     3481 agcgccatgc tcggcgacca catcaagaag aacgtggacc ggggattgag cgacgtctat
     3541 tggtatcggg tttcgaaata cgtcgccgcc gcgtattggg tctcgcagtc cgccgccacc
     3601 acggtgcggt tgtggccgaa aaaagagaaa acccctccgg acgtgaacgg ggccctggcg
     3661 cgttcgttcg ttttgcccgg atggggtttg atctacagcg gctactatga gacggggttc
     3721 aacgtgatgg cgttggaggg gttcgtcctg tattggggga ccaaatcgga actccccaaa
     3781 aaagaacggc tgaatttggt cgctttctcc cattgggggc aggtggtgtt gacgggggtt
     3841 ctggcgaagt acaaggccgc cggtctgcgt gcggcgctgg tgccggcgga cgacggcgcg
     3901 ggcgtcgtgt tcgttctgca aaaacgtttc ggacgggaag aatgaaattg aacgagttgt
     3961 tcgacctgtc tcccggcaaa gtggaggatc tgcgtcaacg cgtggcgcgg ctcggaatca
     4021 atctggcgga ggtggaagag gagttcacgc gcggcggggg gaaaggcggg caaaagatca
     4081 acaagacggc caaccgggtg gtgctccatt accgtccctt gcagatcgtg gtgcggtgtc
     4141 agcgggaacg gcggcgctcc ctgaaccgtt ttttggcgtt gagggaactg atcgaccaca
     4201 tcgaaatgcg ggtctctccc cggacgtcgc aacggctgaa ggaacatgag cgccggcgtc
     4261 gtcagaagga ccgccgccgg cggcgggggg aacgggccgg cgacaaggcg ggacttccgg
     4321 aaacggggga ggggatttcc ggttaaggga gttgtgtgcg acccatgagg aagcggtcgc
     4381 attcgcgggc ggcggcgcgg ccttcgtgga tggcccacac caccaggctt tggccgcggc
     4441 gggcgtcccc cgcggcgaaa attttcggca ccccggtatg gaaccgcccg tcatccgccc
     4501 ggatattgga acgttcgtcc tgcggcaccc ccagttgttt caggaggccg tcctccgggc
     4561 ccaagaatcc catggccaga agcaccagtt gtgccggtcg tattttttcc gatcccggaa
     4621 tttccgacgg actgaaacgg cccttctcgt cttttttcca ttcgacccgt acggtgcgga
     4681 cttctttcac gcgtccgccg gggtccccca aaaattcctt ggtctggatc aaataattcc
     4741 gcgggtcgga cccttgtagc gcggcggcct cctcctggcc atagtcggtt ttgaggattt
     4801 tcggccattg gggccagggg ttgtccggag ggcgtcggtc gggcggttgg ggcaggatct
     4861 cgaactggac cacgcttcgg catccgtgcc gcagcgccgt tccaacgcag tccgtgccgg
     4921 tatcgccgcc gccgatcacc accacgtctt tccctttggc cgagatgtag ttcccatccc
     4981 gaaggccgct gtccaaaagg ctcttggtgt tggcgtggag gaattccacg gcataatgga
     5041 tgccgtccag tcggcggccg ggaacgggga ggtcgcgggg ccgggtggcg ccggtgcaaa
     5101 ggacgacggc gtcgaattcc ttcaggagct tttcggccgc gtagtttttt cccacctcgg
     5161 tgttcgtcag gaaattcaca ccctccgcgg tcaggagatc cacccgccgg gtcacggtgc
     5221 gtttgtccag tttcatgttg gggatgccgt acatcaagag accgccgatg cggtcggcgc
     5281 gctcgaagac cgtgacccaa tggccggcgc ggttgagttg cgcggcgcag gccagccccg
     5341 ccggacccga ccccaccacc gccacccgtt tgcccgtgcg cagggagggc ggttcggcaa
     5401 cgacccatcc cgcgtcgaaa ccctcctcga tgatggaaga ttcgatgttc ttgatggtca
     5461 ccggcggctc gttgatgccc agcacgcagg acccttcgca gggggcgggg cagacgcggc
     5521 cggtgaactc ggggaagttg ttcgttttgt gcagacggtc cagggcgtcc ttccagaggc
     5581 cgcggaaaac caggtcgttc cattcgggga tgaggttgtt gagggggcat ccggtggtca
     5641 tcccgttcaa gagcatgcct gtatggcaaa aaggcacgcc gcagtccatg cagcgggcgc
     5701 cctgtttccg gagtttttct tcgggcatgg gctcatggaa ctcgttccag tggcggaggc
     5761 gctcccgcgg actggcgtcg tgcgggagtt cccggttgaa tgtcagaaac cccgtcggtt
     5821 ttcggatgcc cagcggggtc gggccgggct ggcccgtttc ggttttatga ggtctcatgg
     5881 ccgttccctc tccggtcagt ttccgcccac gcgggagacg tcccgcgcgt tcgcttcaaa
     5941 ggccgccatc acggcttcct cgcccgccag ccccgaagcg tgtacgcgtt tgatggcggt
     6001 gatcatgcgt ttgtagtcct tcggcatcac cttgatgaac ttcgccgcgg tcttctccca
     6061 gtcggcgagc agtcgccagg ccagggcgct cttggtccat tcggcatggc ggcggaccat
     6121 cgcctccact tccttgatgt cttcggcgtc gagcggttcc aggtccacca tgcgttggtt
     6181 gcatcggagg ggaaagtccc cggtgtcgtc gagcacatag gccaccccgc ccgacatccc
     6241 cgccgcgaaa ttccggccgg tccggccgag cacgaccacg tggccgccgg tcatgtattc
     6301 gcaggcgtgg tcccccacgc cttccaccac ggctctcgcc ccgctgttgc gcacacaaaa
     6361 acgctccccg gccacgccgc ggatgtaggc ttcgccgccg gtggccccgt agaaggccac
     6421 gttgccgatg atgacggttt cttccggcgc gaacgtggag tccttcggcg ggaaaacgat
     6481 gatcttcccg ccggagagcc ccttgcccag gtaatcgttg gtgtctccct cgacgaggaa
     6541 agtcatgccg cgcggcagga aggcgccgaa actttgtccg gcggtccccg tgaagtggac
     6601 gcggatggtg tcgtcgggca ggccctgggc tccgtaacgg cgggtcagct cgctgcccac
     6661 gagggtgccc accacgcggt tcttgttctg gacaggcagg gaagccgtca ccggttcccg
     6721 ccgctccagg gcgggggcgc agagtcccaa cagcacctgc cggtccaggc ttttgtcgag
     6781 cccgtggtcc tgggcggtct ggcaatagcg gcccacctcc ggcgggacgg gcggttggta
     6841 gaggatttgg gaatagtcga gccctttggc tttccaatgg tccacggccc ggcgcatcgc
     6901 cagtcggtcg gcccggccga ccatctcgtt gacggtgcgg aaccccagtt gggccatgta
     6961 ctcgcgcact tccgaggcga tgaaccgcat gaagttcacc acgtgggccg gatcgccggc
     7021 gaattttttc cggagttggg ggtcctgcgt ggccacgccc acgggacagg tattctggtg
     7081 gcagacccgc atcatcacgc agcccagcac caccagcggc gcggtggcga acccgaactc
     7141 ctcggcgccc aacaacgcgg ccaccaccac gtcccggccg gtcttgagtt gtccgtcggt
     7201 ctccaccacc acccggctgc ggagattgtt gaggagcagg gtctgatggg tctcggccag
     7261 tcccagttcc cacggcaacc ccgcgtgctt gatgctgctt tggggggagg cgccggtgcc
     7321 gccgtcgtag ccgctgatga gcaccacgtc ggcgtgcgcc ttggccacgc cggcggcgat
     7381 ggtccccacg cccacttccg acaccagctt cacgctgatg cgggcccggg ggttggcgtt
     7441 cttgaggtcg tggatgagtt cggccaggtc ctcgatggaa tagatgtcgt gatgcggcgg
     7501 cggcgagatg agtccgacgc ccggcgtgga atgccgcgtc ttggcgatcc acggaaaaac
     7561 cttgtgtccc ggcagctggc cgccttcgcc gggtttggcc ccctgcgcca tcttgatttg
     7621 gatttcctgg gcgttgacca ggtagaggct ggtgacgccg aaccggccgg aggccacctg
     7681 cttgatggcg ctcttgcgtg aatccccgtt ggggtccggc acgaaccggg ccgggtcttc
     7741 cccgccttcg ccggtgttgc ttttaccggc gatgcggttc atggccacgg ccagcgcctc
     7801 gtgcgcctcc tgactgatgg agccgtagga catcgcgccc gtcttgaacc gtttgcatat
     7861 gtcttcgacg gactccacct cctccagcgg gaccggctga gtggaaggcg tgaggtccaa
     7921 gaggccgcgc agggtgcaga gggtttttgt cctgtt
//
LOCUS       DAOTFD010000111         7955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6439_length_7955_cov_4.198608, whole genome shotgun sequence.
ACCESSION   DAOTFD010000111 DAOTFD010000000
VERSION     DAOTFD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6439_length_7955_cov_4.198608"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5079_07185"
     CDS             complement(<1..550)
                     /locus_tag="P5079_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29807.1"
                     /translation="MTHAAHAPRRRLTVSSLFDHLTPETLWAASLGGLNLLTRRMGDE
                     SRYRWARRISRTARWVAPGKWYAVESNLALVNRWAGAHFQTHKVFENFVLTLSDFLSG
                     AKPVVHVEGREKAEAARAVGKGAVFLTSHLGHWELGGRILADWGWPVTAVYQPYRSAA
                     MHDFIHKRRSPGLRYLAVGKGAA"
     gene            complement(547..1734)
                     /locus_tag="P5079_07190"
     CDS             complement(547..1734)
                     /locus_tag="P5079_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY29808.1"
                     /translation="MSALKKRVLFLYIAISSGHQRAAEALMGAVSRLNPHIVCHGIDT
                     IAETYPIAGRFIGRMYLEMLKYTPQIWEFLYDNPAVEGTTRELREVMGAMNTRKIYRL
                     LKRYRPRCLVCTQAVPLSVLANLKRRGRIKLPLVGVVTDFDAHSYWLSRHVDLYLVAT
                     DEIKRKMVREGIKEHRVAVTGIPIDPHFAVPGNKVDERDRLGLHPDKPTVLVTGGSRG
                     LGPLEDIVSSLRRAMPSLQVIAVCGHNTHARDILQERFGNDRHVRVFGFTRNLPRFMD
                     AADLLVSKPGGMTCAESMAKGLPLVMIRPIPGQEERNARYLLKHGAAARAQTIGELVS
                     TVQNLLKNREHLRQIAGRAKALAKPDAAPEAAEAILKILREPLTSRPAPHDRARTHSS
                     AAV"
     gene            complement(1752..2258)
                     /locus_tag="P5079_07195"
     CDS             complement(1752..2258)
                     /locus_tag="P5079_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY29809.1"
                     /translation="MTITNDEARWLYKALKQIEFLKFCSIGEIEELAQNLVKKTFKTG
                     ETIVRQGDPGDYLFLLYSGEVSIQARSAISKRHELTRLGAGNYFGEMALVSREPRNAT
                     VVATQDSEAFLLYAAEFRKLLDKNQDLARTLEDLVARRKAARELELRRSYQQSQALLS
                     RLKRWLGF"
     gene            complement(2535..2606)
                     /locus_tag="P5079_07200"
     tRNA            complement(2535..2606)
                     /locus_tag="P5079_07200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2572..2574),aa:Pro,seq:ggg)
     gene            complement(2661..3050)
                     /locus_tag="P5079_07205"
     CDS             complement(2661..3050)
                     /locus_tag="P5079_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY29810.1"
                     /translation="MVLPFPPEKEYFSIGEAGRLTGTKPYILRYWESKFGLVRPARRE
                     SGQRKFTRQDVETIARIKELLYEKRFTIEGAKKYLRDLARRGPVQMSLELNETSAAVD
                     TLRDVKKDITGLLKELKKRSFSSAGSS"
     gene            complement(3224..3481)
                     /locus_tag="P5079_07210"
     CDS             complement(3224..3481)
                     /locus_tag="P5079_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY29811.1"
                     /translation="MNKADLILSVSNVVSTLTESKAAVNRVFDSMRKALQEGDKVVVQ
                     GFGSFHVVMRKSKPARNPRTGEPVTIPPRRRIKFKMAKDLL"
     gene            complement(3478..4551)
                     /locus_tag="P5079_07215"
     CDS             complement(3478..4551)
                     /locus_tag="P5079_07215"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRY29812.1"
                     /translation="MKKKSPDLPSPENERIAVIGGGSWGATLANLLAQKGHAVSVWEF
                     LPETVKHLRETRTLKVLPQLRLHPSVEVTHDLPAALREKRLVVMAVPSAHVRATCRAI
                     QEGNVFPPNAWAVSVTKGIENDSLKRMSEIIAEEIPAAKNRIAVLSGPSHAEEVAKSV
                     PTAVVVAGPDRFAERVQKIFNTDTFRVYSSTDFIGVELGGAIKNIYAIATGICDGLGL
                     GDNTKAALMTRSLNEMIRLGMACGARGLTFFGLSGMGDLIVTCGSRHSRNRFIGEKIG
                     RGESLKQALSEMTMVAEGVPTAKSAYQLAQRFHLELPIVNEVYRCLYEDKPARRALQD
                     LLTRPVAGEMWQVENLFKKGMTL"
     gene            complement(4532..5149)
                     /gene="plsY"
                     /locus_tag="P5079_07220"
     CDS             complement(4532..5149)
                     /gene="plsY"
                     /locus_tag="P5079_07220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRY29813.1"
                     /translation="MTALAPAGLWLVACYLVGSLPTGYLLGRFLKGIDIRQVGSRNPG
                     ATNVFRSVGKTAGIATLLIDIFKGWVVTWLSLRFFPGEFLPLLGGLAAVAGHSWSPFL
                     SFRGGKGVATSAGVFFALLPTPTLAALTVFAVVFGLSHYVSAGSLAGAVALPSVALWL
                     AGWGPRSGLALLVAALIVVKHIPNIRRLLKGEELAVRFTHEEKKP"
     gene            complement(5229..6554)
                     /gene="der"
                     /locus_tag="P5079_07225"
     CDS             complement(5229..6554)
                     /gene="der"
                     /locus_tag="P5079_07225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRY29814.1"
                     /translation="MEKQPALPRATVVLVGRPNVGKSTLFNRLCRKRRALTSPIPGTT
                     RDWIEGAVLWGGRPFRVVDTGGYAPGDDDILASVRVKVEELVRAAQVAVWVLDGQEGL
                     TPADRAVGRWLRRHAAHVLVAVNKLDDQKKNIHSSEFFSLGFQKVIPLSASHGRNINQ
                     LLDEIVPFLPEKDDRPSAAPDRGRFAIVGRPNVGKSSLLNRLLGEERAIVSNQPGTTR
                     DTLDTALDWNGKKFLFMDTAGLRAKKSRAVGLEGLTRLMTEKALEDCQAALLLLDAPD
                     GILEGDAAIARLIHEKNRACVVAVNKWDAVAEKNRAAAWFKERLAGDMPFLGFAPLLF
                     VSAKEGTNVPHLMEQLWAARTEFLRSYDKDELESFFWTEIQNRPYSHHGKKLVFRGAE
                     QVADAPPTFVLHTSLSDEDIHFSYLRHLENVFRRRFQALGTPLLFRFRR"
     gene            complement(6557..7093)
                     /locus_tag="P5079_07230"
     CDS             complement(6557..7093)
                     /locus_tag="P5079_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY29815.1"
                     /translation="MTLANKITLLRLLLTPVFVIGLLEGARGWPTALFILSVLTDMAD
                     GLAARLKKEKTTLGGLLDPAADKLLLVTTYLVFAHLGRVPLWIFVVIFSRDLFIVLGW
                     SLIFILTRKILITPRWLGKSTTFMEMSTAVLLLLSPAHPLVDVFLWITIALAGASLAD
                     YTRLGMQKMNEMDAVPAR"
     gene            complement(7090..>7955)
                     /gene="hflX"
                     /locus_tag="P5079_07235"
     CDS             complement(7090..>7955)
                     /gene="hflX"
                     /locus_tag="P5079_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRY29816.1"
                     /translation="FEQQTGGARGGIGTRGPGERKLEVDQRHIRERIRRIKREIEHVR
                     LQRDLQRQNRRRIPLPQVALVGYTNVGKSTLLNFLTRSDGIYADDKLFATLDPTARRV
                     KLPGGRAALFVDTVGFIQKLPHHLVAAFHATLEELNNADLLVCVVDAADPAWTAHLRV
                     VQEVVKSLRADHLPQIVVFNKADLLKNTDRAARQREGRLLLSAVTGEGMDAFLSAVES
                     RLDRPLLEKEFVLPYRRRDLLSVIYHTGRILSETPAVRGTRLRVRIDAQNWGHIQKEL
                     RPDAKPARTAS"
BASE COUNT         1350 a         2216 c         2882 g         1507 t
ORIGIN      
        1 cggcagcccc tttcccgacg gccagatagc gcaatcccgg ggaacggcgt ttgtggatga
       61 aatcgtgcat cgcggcggaa cggtagggct gatacaccgc ggtgacgggc catccccaat
      121 cggccaggat gcggccgccc agttcccaat ggccgaggtg cgaggtgagg aacacggccc
      181 ctttgcccac ggcgcgggcg gcctcggctt tctccctccc ctccacgtgt acgacgggtt
      241 tggcgccgga cagaaaatcg gagagggtca ggacgaaatt ctcaaagacc ttgtgtgtct
      301 ggaaatgggc gccggcccac cggttgacca aggccaggtt tgattctacc gcataccact
      361 tccccggcgc aacccatcgg gcggtccggc tgatgcgccg ggcccagcga tagcggctct
      421 catcgcccat gcggcgagtc aacaggttga gccctccgag ggaggcggcc cagagcgttt
      481 ccggggtcag gtggtcaaag agcgaagaaa ccgtgagccg gcggcgcggc gcgtgtgcgg
      541 cgtgcgtcac accgcggccg aggaatgcgt tcgggcccgg tcgtgcggcg ccgggcggga
      601 ggtcagcggc tccctcaaaa tcttcaggat cgcctcggcc gcctcggggg cggcgtcggg
      661 tttggcgagc gccttggcgc gaccggcgat ctgccggaga tgctccctgt tcttgagcag
      721 gttctgcacg gtgctgacga gttccccgat ggtctgcgcg cgcgccgcgg cgccgtgctt
      781 gagcagatag cgggcgttcc gttcctcctg gccggggatg ggacggatca tcaccagtgg
      841 gagccccttg gccatgcttt cggcgcaggt catgccgccc ggcttggaaa cgaggaggtc
      901 ggcggcgtcc atgaagcggg gcaaattgcg cgtgaacccg aacacccgca cgtggcggtc
      961 gttgccgaaa cgttcctgga ggatgtcgcg ggcgtgggtg ttgtgtccgc agacggcgat
     1021 gacctggagg gacggcatgg cgcggcgcag actggagacg atgtcttcca gcggaccgag
     1081 tccgcggctt ccccccgtca ccagcacggt gggtttgtcc ggatgcaggc ccaggcggtc
     1141 ccgttcatcc accttgttcc cggggacggc gaaatggggg tcgatgggga tgccggtcac
     1201 cgccacgcgg tgttccttga tgccttcccg caccatcttc cgtttgatct cgtcggtggc
     1261 cacgaggtag aggtccacgt ggcgggagag ccaatagctg tgggcgtcga aatcggtgac
     1321 cacgccgacc agcggcagtt tgatgcgtcc gcgccgtttc aggttcgcca gcacgctgag
     1381 gggcacggcc tgggtgcaga cgagacagcg cgggcggtag cgtttgagga gacggtaaat
     1441 tttccgtgtg ttcatggcgc ccatcacttc gcggagttcg cgcgtggtgc cttccacggc
     1501 aggattgtcg tagaggaact cccatatctg cggggtgtat ttcagcatct cgagatacat
     1561 gcggccgatg aagcggccgg cgatgggata ggtctcggcg atggtgtcga tgccgtggca
     1621 gacgatgtgc gggttgagcc gggagacggc gcccatgagc gcctcggcgg cgcgttggtg
     1681 tccgctggaa atggcgatgt agaggaagag gacgcgtttt ttcagagcgg acatttttta
     1741 cgggccgggt ttcagaatcc gagccagcgt ttcaggcggg agaggagggc ctgggattgc
     1801 tgatagcttc ggcggagttc cagttctcgg gcggccttgc gccgggccac gaggtcttcg
     1861 agggtccgtg ccaagtcctg gttcttgtcc agcagtttcc ggaattcggc ggcgtagagc
     1921 aaaaaggcct ccgagtcctg ggtggccacg acggtggcgt tgcggggttc gcgggagacg
     1981 agggccattt cgccgaaata gttccccgcg cccaggcggg tcagttcgtg ccgtttggaa
     2041 atggcggagc gggcctggat ggagacttca ccggaataga ggaggaaaag gtaatcgccc
     2101 gggtctccct ggcgcacgat ggtttcgccc gtcttgaagg tttttttgac caggttctgg
     2161 gcgagttcct cgatctcccc gatggaacag aatttcagga actcgatttg tttgagcgcc
     2221 ttgtagagcc agcgggcttc atcgtttgtg atggtcatga gacgtttttc ggcgaaaaaa
     2281 cagccgtcga aacggcctcc ttcacagagt aggctgcgtg gttcatggcg gctccatttt
     2341 tgccggctgt ccagtcgggg cggtggggat tcccttcggg cctgcccgcg gcacacggcg
     2401 ccccaggacc tccgcggctc ctccgccttc gtggagcggc cgcccgctcc actcaataaa
     2461 cggttgcaga gagcaaccgg tggccccccg cgcacccaag tcgccgggcc gcggtttttt
     2521 ttcgagccac tccatcgggg cggtggggat tgaacccacg acctctcgca ccccaagcga
     2581 gtgcgctacc actacgctac gccccggtag cgtggctcga aaaaatcgat cccctcgggc
     2641 agcgtggagc cggtaaaatt ttaagacgaa cccgccgacg aaaaacttcg tttcttcaat
     2701 tctttgagaa ggcccgtgat gtcttttttg acatcccgca gggtgtccac cgccgcggag
     2761 gtctcgttga gttccaggga catctggacc ggtccccgtc gggccaggtc gcgcaggtat
     2821 tttttggcgc cctcgatggt gaaccgtttt tcgtagagca gttccttgat gcgcgcgatg
     2881 gtctccacgt cttgacgggt gaacttccgt tggccggatt cgcgccgcgc cggccggacg
     2941 agcccgaatt tggactccca atagcggagg atataaggtt tcgtgccggt caggcggccg
     3001 gcttcaccaa tggaaaaata ttctttttcc ggcgggaatg ggaggaccat acgaaaacat
     3061 attcaaacat aaaggacggg tcgaagtcaa agaaaggaag gggcgtcggt catggttttt
     3121 gtgttggcca caaaacccgt tcgctctccc atgggtcacc gagtgacggt catggttttt
     3181 gtgttggcac acaaaaacca ttccctcgcg gcccctcgct cggtcacagt aaatctttgg
     3241 ccatcttgaa tttgatgcgg cggcgcggcg ggatggtgac cggctcgccg gtgcgggggt
     3301 tgcgcgccgg tttgcttttc cgcatgacga cgtggaaact gccgaacccc tgcacgacca
     3361 ccttgtcccc ctcctgcagg gccttgcgca tggaatcgaa aacgcggttc accgcggcct
     3421 tggattccgt gagggtgctg accacgttgg agacgctgag gatcaggtcg gccttgttca
     3481 tagggtcatt ccttttttga agaggttttc cacctgccac atttcgccgg ccacgggccg
     3541 cgtgaggagg tcctgcaggg cccgccgggc cggtttgtct tcgtagaggc agcgatagac
     3601 ttcattgacg atggggagtt ccaggtggaa tcgttgagcg agctgatagg cgctcttggc
     3661 ggtggggacg ccctcggcga ccatggtcat ttccgagagg gcttgtttca aggattcccc
     3721 gcgtccgatt ttttcgccga tgaaccggtt gcgggaatgg cgcgagccgc aggtgacgat
     3781 gaggtcgccc atgccggaga ggccgaagaa ggtgagcccg cgggcgccgc aggccatgcc
     3841 gaggcggatc atttcgttga ggctgcgggt catgagggcg gctttggtgt tgtctcccag
     3901 gcccaggccg tcgcagatgc cggtggcgat ggcgtagatg ttcttgatgg cgccgcccag
     3961 ttccacgccg atgaagtcgg tgctgctgta gacccggaaa gtatcggtgt tgaaaatttt
     4021 ctgcacgcgt tccgcgaaac ggtccggtcc ggcgacgacc acggcggtgg ggaccgattt
     4081 ggcgacctct tccgcgtggg acggcccgga aaggacggcg atgcggtttt tcgccgccgg
     4141 gatctcctcg gcgatgatct cggacatgcg tttcagggaa tcgttctcga tgcccttggt
     4201 gacgctgacc gcccaggcgt tcggcgggaa gacgtttcct tcctggatgg cgcggcaggt
     4261 ggcgcggacg tgggccgaag gcacggccat gaccacgagg cgtttctccc gcagggccgc
     4321 ggggaggtcg tgggtcacct cgacggaagg gtgcaggcgc agctgcggca ggactttcag
     4381 ggtgcgggtt tcccggagat gtttgacggt ttcggggagg aattcccaaa cggacacggc
     4441 gtgccccttt tgggccaaga ggttggccag agtggcgccc caactgccgc cgccgatgac
     4501 ggcgatgcgc tcgttttccg gagaggggag gtcagggctt tttttcttca tgggtgaacc
     4561 ggacggccag ttcttcgcct ttcagcaggc ggcggatgtt gggaatgtgc ttgacgacga
     4621 tgagcgcggc caccagaagc gcgaggccgg accgggggcc ccatccggcg agccacagcg
     4681 ccacggaggg cagggcgacg gcgccggcca gagaacccgc cgaaacataa tgggagaggc
     4741 cgaagaccac ggcgaacacc gtcaacgcgg ccagggtggg cgtcgggagg agggcgaaga
     4801 agaccccggc ggaggtggcc acgccttttc cccctcgaaa agagaggaag ggagaccagc
     4861 tgtgtccggc cacggcggcc agcccgccca aaagcggcag gaattccccc gggaaaaacc
     4921 gcagggacag ccaggtgacg acccagccct tgaagatgtc gatgaggagc gtggcgatgc
     4981 cggccgtttt gccgacggac ctgaaaacgt tggtggcgcc ggggtttcgg gaacccactt
     5041 ggcggatgtc gatgcctttc aggaagcgcc ccaggaggta tccggtgggt agggacccga
     5101 cgagatagca ggcgacgagc cagaggccgg cgggcgcgag ggcggtcata tccgtggagc
     5161 ggccgaaaaa atcattttca taggacaaga aatagcattt ccgaaaggtt ttgtctcttg
     5221 ggaggaggtc atcggcggaa tcggaacaac agaggcgtgc ccagcgcctg gaaccggcgg
     5281 cggaaaacgt tttcgaggtg gcggaggtag gaaaaatgga tatcctcgtc ggaaaggctg
     5341 gtgtggagga cgaaggtggg cggggcgtcc gcgacctgtt cggcgccgcg gaagacgagt
     5401 ttctttccgt ggtgggaata cggccggttc tggatctccg tccagaaaaa ggactcgagc
     5461 tcgtctttgt cgtaggagcg caggaactcg gtacgggccg cccagagttg ctccatgagg
     5521 tggggcacgt tggtgccttc cttggcggag acgaagagga ggggagcgaa ccccaggaag
     5581 ggcatgtccc cggcgagccg ttccttgaac cacgcggcgg cgcggttttt ttccgcgacg
     5641 gcgtcccatt tgttgacggc cacgacgcag gcgcggtttt tttcgtggat gaggcgggcg
     5701 atggcggcgt ccccctccag gatgccgtcg ggggcgtcga ggagaaggag ggcggcctgg
     5761 cagtcctcca gggctttttc cgtcatgagg cgggtgagcc cctccagccc cacggcccgg
     5821 gattttttcg cccgcaggcc ggcggtgtcc atgaagagga attttttccc gttccagtcc
     5881 agggcggtgt cgagggtgtc gcgggtggtg cccggctgat tggagacgat ggcgcgttcc
     5941 tctcccagga ggcggttgag cagggaggat tttcccacgt tgggccggcc gacgatggcg
     6001 aaccggccgc ggtccggcgc ggcggacggg cggtcgtctt tttcgggcag gaacggaacg
     6061 atctcatcga gcagttggtt gatgttgcgg ccgtgactgg ccgaaagagg gatgaccttc
     6121 tggaacccca ggctgaagaa ttccgacgag tggatatttt ttttctggtc gtccagttta
     6181 ttgaccgcga caaggacatg ggcggcgtgt cggcggagcc agcggcccac ggcgcggtcc
     6241 gccggggtga gcccctcctg gccgtcgagc acccacaccg cgacctgcgc ggcgcggacc
     6301 agttcctcca ctttgacccg gacggaggcg aggatgtcgt cgtcgcccgg ggcgtagccg
     6361 ccggtgtcga ccacgcggaa cgggcggccg ccccacagga cggcgccctc gatccagtcg
     6421 cgcgtggtgc cggggatggg cgaggtcaag gcgcggcgtt ttcggcagag gcggttgaag
     6481 agggtggatt tccccacgtt gggccggccg acgagaacga cggtggcgcg gggcagggcc
     6541 ggttgttttt ccatggtcag cgggccggaa cggcgtccat ttcgttcatt ttttgcatgc
     6601 cgagccgtgt gtagtccgcc agagaggcgc cggcgagagc gatggtgatc cagaggaaaa
     6661 catcgaccag gggatgcgcc ggagacagca ggaggaggac cgcggtggac atttccatga
     6721 aggtggtgct tttgccgagc caccgcgggg tgatcaggat cttcctggtc aggatgaaga
     6781 tgagactcca tcccagaacg atgaacaggt cgcgtgaaaa gatgacgacg aagatccaca
     6841 gggggaccct gccgagatgc gcgaagacga ggtaggtggt caccaggagc aatttgtcgg
     6901 ccgcggggtc gaggagtccg ccgagggtgg ttttttcttt cttgagccgg gcggccagcc
     6961 cgtcggccat gtccgtgagg acggaaagga tgaagagggc ggtgggccag ccgcgggccc
     7021 cctcgaggag cccgatgacg aacacggggg tgaggaggag gcgcaaaagg gtgatcttgt
     7081 tggcgagggt cacgaggcgg tccgggccgg tttggcgtcg gggcggagtt ctttctggat
     7141 atgcccccaa ttttgggcgt cgatgcggac gcgcaggcgc gtgccccgga cggcgggggt
     7201 ttcggagagg atgcgtccgg tgtggtagat gacggaaagg aggtctctcc ggcggtaggg
     7261 gaggacgaat tctttttcca gcagaggccg gtcgaggcgg gattccacgg cggagaggaa
     7321 ggcgtccatg ccttcgcccg tgaccgcgga gaggaggagc cgtccctcgc gttgccgcgc
     7381 ggcgcggtcg gtgtttttga ggaggtcggc cttgttgaac accacgatct gcgggaggtg
     7441 gtccgcccgg agcgatttga cgacctcctg caccacgcgg aggtgggcgg tccaggcggg
     7501 gtcggcggcg tccaccacgc agacgagcag gtcggcgttg ttgagttcct ccagggtggc
     7561 gtggaaggcg gcgaccaggt ggtgcggaag tttctggatg aaaccgacgg tgtccacaaa
     7621 aagggcggcg cgtccgccgg ggagtttcac gcggcgggcg gtggggtcca gcgtggcgaa
     7681 gagtttgtcg tcggcgtaga ttccgtcgga gcgggtgagg aaattgagca gggtggattt
     7741 gcccacgttg gtgtagccga cgagggccac ctgggggagc gggatgcgcc ggcggttttg
     7801 gcgctgcagg tcccgctgga ggcggacgtg ttcgatctcg cgtttgatgc ggcggatgcg
     7861 ttcccggatg tggcgctggt ccacctccag tttgcgttcg ccgggtccgc gggtgccgat
     7921 gcctccccgc gcgccgccgg tctgttgttc gaagc
//
LOCUS       DAOTFD010000112         7892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6505_length_7892_cov_4.609800, whole genome shotgun sequence.
ACCESSION   DAOTFD010000112 DAOTFD010000000
VERSION     DAOTFD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_7892_cov_4.609800"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1127)
                     /locus_tag="P5079_07240"
     CDS             complement(198..1127)
                     /locus_tag="P5079_07240"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY29817.1"
                     /translation="MTGALVIFTGLFLSLKSSGFAGNGSASAQFLKLPTDTRGAAMGE
                     AFGAAATGAASQFWNPAGLVHADALDVNFTHVVMLDDMAYSSLGAATGLKNNGAIALQ
                     LSGLTQPAIDVYDVMESRTGSTITPSDQMIAIGLAKRIKRVPVGVNFKWLQSSLGAAN
                     ASGLAGDAGILIESIKNMRFGIGLQNFGQKIKYDAAEEPLPLKVYVASAYARPKIFMA
                     VAQLESTRENSLVAKTGIETKFQIEKSIISLRGGISSENVETGGLAMLRLGFGVAIKY
                     INVDYTWAPVGELGDIHWIGMGFRYGGTNSTKP"
     gene            complement(1139..3505)
                     /locus_tag="P5079_07245"
     CDS             complement(1139..3505)
                     /locus_tag="P5079_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29818.1"
                     /translation="MPHSLFRTGTDNFLISRVSHRVHPQKILRFYRPLLLFATLLLST
                     INIFPAEIPSGHTEVFVLFNTHTQNEYDAVLLRLRKLGAIIAKKLPPTAVCLYIDNKT
                     LNSFPYPAETILNTSHEIPSDFTQKLQDKIAIAAARAWNAKFNRKREHPPGSPNPLIN
                     DMILEPQFSDGQTGIFQAPYNVDLSQTSEFMMGSVAVSIIFVESGPESILDYEDWDSA
                     REQTVINKIKAGLNWYAGKYPDAHLSYQYEYHYGRTDPAYQVAWEPIFHDGEFCWCWI
                     NEIMMSLGITDDPYSNDRNNVRTYNRGLRDRYASDWAFTIFVVDSVNDVDGQFDDGMF
                     AFSYPGGSYMVMTYDNDGWGIDAMDVVMAHETGHIFWAWDEYSAAGKSANDKSGYLNY
                     PNGNNEVGGVINETTCIMRGNSSALAGNTICSYTKGQIGWVDSDGDTIPDIIDTAAIN
                     VGGAEGGLGVTNVNATVTATMQPLVENRNTQNLSGNDVTIRKIADLRWRLGSGTYQSP
                     IFDTGSTEVEKKFSVLTGNLADGQYLLDVKMTDSLGHVTTTQKTVDIMSKATINIAAI
                     GETAGSIPTADGTINYTLPAGTFQTAKTIEMKFPQSVPLGKLPYTDWSKTGIGLELTT
                     DNGEQPQKSIHIVQSYSPTYDSVMLAPTKFAYYDPNTSYWHILPTAINRTSRKITWET
                     DHLSIFQLLLLAIPTSTENVIASPNPFRKSQGHTEITFSRLPEFSTVRIYTPLGGRVK
                     EISAFGSPPMAIWDGKNENGEAVTSGTYLFHVEGGAGKGKNGRLVIIR"
     gene            3928..4569
                     /locus_tag="P5079_07250"
     CDS             3928..4569
                     /locus_tag="P5079_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY29819.1"
                     /translation="MSRPSGDADRRLVRAGRELLPRTGLSGLNVRRLADRAGVNLGMF
                     HYHFKTKRVFVRQVLQEIYEEFFRGFSLETSAEGPAIERLRRALQILGRFSRDNRALV
                     LCLLADGVRGEAEALRFAKHNLHRHVSILVKLIVRAQVAGDLPKVPPPALLGFLMGGV
                     AVPNLVAGLLERQTGGGLKELPLRLITSGLLSDESVDRRVEWALAAFAKRRRP"
     gene            4566..5933
                     /locus_tag="P5079_07255"
     CDS             4566..5933
                     /locus_tag="P5079_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY29820.1"
                     /translation="MRRFVGAALCGVLAWGFPAGPAGAQDSLRLGLRETEERALKNSE
                     SLRAAERELAAARARERGQRAPLFPQVSLDGSFRYVSEVAAFRPAAGGPEVKLGDNEN
                     YSVGPTLSWLMWDSGGSRNRRRSASARRRAKENEAEAARRNVLLEARLDYFQVQLALE
                     QVRLLADSLALAEAQYKDISLKQKAGAVSRLDALSAHQEVLSRQRQLRQAQTELSVAL
                     RTLLARTRPETPVDPSVTGPVDGLGRWPSLAETPTLLLGLEDLSVTRGRLEPAVAAAP
                     DTEHPQRMALLQTAESARRAARALAGGGGPVLQFSARTSLDYPNGPKLETIHQNTVGL
                     SARLPLFEAGKSKREAEEQNEQAAALEARRDEAAVELDRAWRQAHDQIKGLTAQLEIA
                     RRSVDETEELARLTYDSYTAGRSSFIEVQSANLRALEAKVVAARTEVQILMQLAIVDG
                     LSRKG"
     gene            5938..6663
                     /locus_tag="P5079_07260"
     CDS             5938..6663
                     /locus_tag="P5079_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY29821.1"
                     /translation="MKKKILIGLLAVAAGIAAWRLSSPREFFYAGTVEATEVDVSARV
                     ASPVAAYEVFEGAEVSEGQTLVRLSGEDIRLAKEQTERDFQRAKKLYEGGSVSQEVFD
                     RLRFLRDDAALRAGWCEVRSPLAGTVLRVYHEAGEQAMPGTKLLTLADLREVWAYVYV
                     PQPLLARISLGQEVEGLLPETGRALKGTIRFIRSEAEFTPKNVQTREERTRLVYGVKV
                     VFPNPERVLKPGMTVEVRLPRGK"
     gene            6679..7410
                     /locus_tag="P5079_07265"
     CDS             6679..7410
                     /locus_tag="P5079_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29822.1"
                     /translation="MFLGVEVRDLHKKLGAQRALAGFSSDFSAGALHGVIGPDGAGKT
                     TLLRLLAGLLRPDAGEIVFTDGGRPAPFASVRPTLAYMPQQQSLYPDLTCSEHLDFFR
                     DLYRLPKDVYRERRGKLLHLTRLEPFQSRRAGQLSGGMYKKLGLMCALLQSPSAVLLD
                     EPTNGVDPVSRREFWELLYGLVERKTLVVVSTAYMDEAERCGVVHLMDAGRRISSGEP
                     RALLEKEGASGFDEIFRRRAAEARR"
     gene            7407..>7892
                     /locus_tag="P5079_07270"
     CDS             7407..>7892
                     /locus_tag="P5079_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29823.1"
                     /translation="MNGPGVAVEVSGLTVRFGDFTAVDDVSFRVARGEIFGFLGANGA
                     GKTTTIRTLCGLLAPSAGRVRVAGLGFEDGGRAIKARVGYMSQRFTLYNDLTVRENLA
                     FAAGLRKMEEGVLARRTRELFAFVGFDRPMTELARDLPGGVKQKLALAVTLLHEPEIL
                     FL"
BASE COUNT         1497 a         2138 c         2541 g         1716 t
ORIGIN      
        1 tctccgaaaa aacggctcgg tcaaggcggc cttaaggggg gggtgaagaa accgtcagcg
       61 cccacggcgg ggaggcgttg atgagccgaa gttaaaaagg gtgtcggaat tgacagttga
      121 cgcttgtcgc gttattgcag gatgcgttgc tgaacctggc aactgtcaag acaggcatct
      181 gtccttgcag tttctgctta aggttttgtg gaattggttc caccgtatct aaagcccatc
      241 ccgatccaat ggatgtctcc gagttccccc accggcgccc aggtataatc gacattgata
      301 tattttatgg cgacaccaaa cccaagtctc aacatcgcta ggcctcctgt ttcgacattt
      361 tcgcttgaga tgccacctct taatgagata atagattttt ctatctgaaa ttttgtttcg
      421 atacctgttt ttgccacgag ggagttctct cgtgtgcttt caagttgtgc cacggccata
      481 aatattttcg gcctggcata ggcgcttgcg acgtatactt ttagcggtaa tggttcttcg
      541 gccgcgtcgt atttaatttt ctgtccaaag ttttgcaacc cgattccaaa ccgcatattt
      601 tttatggatt cgattaaaat tcccgcgtca ccggccaggc cggaggcgtt ggccgctccg
      661 aggctcgatt gcagccattt aaagtttaca ccgaccggga cccttttaat tcttttggcc
      721 aggccaattg caatcatctg gtcgcttggc gtaatggtgg agccagtccg actttccatg
      781 acgtcgtaaa cgtcgatggc aggttgggtt aatcccgaga gttgcagggc tatcgcccca
      841 ttatttttca acccagtagc cgcccccaga gacgaatacg ccatatcatc taacataacc
      901 acgtgcgtaa aattgacgtc caacgcatcg gcatggacta gaccagcggg gttccagaat
      961 tgagaagccg ctcccgtggc ggccgcaccg aaagcttcgc ccatagctgc tccacgcgta
     1021 tcggtcggaa gttttaaaaa ttgcgcagag gccgagccgt ttccggcgaa cccactgctt
     1081 ttcaaggaca aaaaaagtcc tgtgaaaatt accagcgcgc ctgtcatctt tatttgactc
     1141 atcgaataat caccaagcgc ccgttcttgc cttttcctgc tccgccctcg acatgaaaca
     1201 gatatgtccc ggatgttaca gcttcgccat tttcattttt cccgtcccat atcgccatcg
     1261 gaggggagcc aaaagcgctt atttctttga cacggccacc cagcggtgta taaatccgca
     1321 cggtggagaa ttcaggtaat ctcgaaaatg ttatttcggt gtgtccctgg gattttctaa
     1381 atggattagg agatgcgatg acattctcag tgcttgttgg tatggccaac agcaagagct
     1441 gaaatattga gaggtgatcc gtttcccagg tgattttgcg ggatgttctg tttatggctg
     1501 ttggcaatat atgccaatac gatgtgttcg gatcataata agcgaatttc gtcggagcga
     1561 gcatcaccga atcataggta gggctatagg attggacgat gtggatcgat ttttggggtt
     1621 gttcgccatt gtcggtggtt agctctaggc cgatgccagt ttttgaccag tccgtatacg
     1681 gcaatttgcc gaggggcacg gattgtggaa atttcatctc aatcgttttt gcagtctgga
     1741 atgtgccggc cgggagcgtg taattgatag tcccatcggc tgtgggaatc gagcctgcag
     1801 tttctccaat agcagcgata ttgatcgtcg ctttgctcat aatatcgact gttttttgag
     1861 tagttgttac atggccaagg gaatccgtca tttttacatc aaggaggtat tgcccatcgg
     1921 ctaaattgcc ggttaatacg gaaaattttt tctccacttc tgtggatcca gtatcaaaaa
     1981 tcggggactg ataggttccc gagccaagcc tccagcgcag atcggctatt tttcgaatag
     2041 tcacatcatt cccggagagg ttttgcgtgt tccgattttc taccagaggc tgcattgttg
     2101 ctgttactgt cgcgttgacg ttggtgacgc ccagcccccc ttctgcaccc ccaacattga
     2161 tcgccgcagt atcgataatg tccgggattg tgtctccgtc agagtctacc catccaattt
     2221 gtcctttcgt gtaagagcaa atggtattcc ccgcaagagc actggagttt cccctcatga
     2281 tgcaggttgt ttcgtttatc acacccccga cctcattatt gccgtttgga taattcagat
     2341 agccgctctt gtcgtttgcc gattttccgg ccgccgaata ctcgtcccac gcccagaata
     2401 tgtgccctgt ttcgtgggcc ataacgacat ccatggcgtc aatgccccag ccgtcgttgt
     2461 cgtatgtcat aaccatatag cttccgccgg gatatgaaaa tgcgaacatc ccgtcatcga
     2521 attgcccatc gacgtcattg acgctatcga caacaaatat tgtgaatgcc caatcggagg
     2581 catatctgtc ccgtagtccc cggttgtatg tccgtacgtt gtttcgatca ttgctatagg
     2641 ggtcgtccgt tatccccagg gacatcataa tctcgtttat ccaacaccag cagaattccc
     2701 catcatgaaa aatcggttcc caggcaactt gataggccgg atcagttcgc ccataatggt
     2761 attcatactg ataggacaag tgcgcgtcgg ggtatttccc cgcgtaccaa ttaagcccag
     2821 ccttgatttt gttgatgacc gtttgttccc gtgcactgtc ccagtcttcg tagtccaata
     2881 tcgactccgg gccgctctcg acaaaaataa tgctgacagc tacggacccc atcatgaatt
     2941 cgcttgtttg cgaaaggtcc acattgtagg gggcttggaa aattcccgtt tggccgtctg
     3001 agaattgtgg ctcaagaatc atgtcgttta ttagcgggtt cggcgagccc ggcggatgtt
     3061 ctctttttct gttgaatttc gcattccatg cccgtgcggc tgctatcgca attttgtctt
     3121 gaagcttctg tgtgaagtct gacggtattt catgggatgt gttcaatatg gtttcagcgg
     3181 ggtaggggaa gctgttcagt gttttgttat caatatacag acagacggca gtgggtggaa
     3241 gtttcttggc gatgattgcc cccagtttgc gcaagcggag gaggacagca tcgtattcgt
     3301 tttgtgtatg tgtgttaaaa agaacgaaaa cctcggtgtg cccggaaggg atttcggcgg
     3361 ggaaaatatt tattgttgag aggaggagcg tggcaaaaag caggaggggt cggtaaaatc
     3421 gcaatatttt ttggggatgc acgcgatgag atacgcggct aatcagaaaa ttgtcagttc
     3481 ctgtcctaaa tagagagtgc ggcatatatg acaatcttat caaattagtc atgcaatgcg
     3541 ataaagggtt aaggaaaaac aggaccaggg ggggtgacgg aacagattcc aagaaacgct
     3601 caggtctgtt cgacaaaacg gccccatgcc tgacggcaaa ggtcaattgg gcggtggcgg
     3661 tcgatgtggg cttctcgacg cgtcaaaaag cgacttgctc gaagaagaat ggggaggcga
     3721 ttcttttgcg gggaggacga caatttcggg ggtgcgcagt gaattgggca acgacgcttt
     3781 tccacaaaca gggcccgtgt ctagcgacac ggacgtatca ctttggggcc tgacgcgctc
     3841 cagcggagcg acgacccgtc aggccttgac aaaatagtta tacgaatgta taattataca
     3901 ggagtataaa aacagcagga ggcgcctatg tccagaccgt ccggagacgc cgaccgccgc
     3961 ctcgtccgcg ccggccgcga actccttccc cgaaccggcc tttccggctt gaacgtccgc
     4021 cgtttggccg accgcgccgg ggtgaacctg gggatgttcc actatcattt caagaccaaa
     4081 cgcgtgttcg tgcggcaggt gcttcaagag atctacgagg agttcttccg ggggttttcc
     4141 ctggagacct ccgcggaggg gccggccatc gagcggctcc gccgcgccct gcagatcctc
     4201 ggccggttct cccgcgacaa ccgcgccctc gtcctctgcc tgttggccga cggcgtaagg
     4261 ggagaggccg aggccctgcg gttcgccaaa cacaacctgc accggcacgt gagcatcctg
     4321 gtgaagctca tcgtccgggc ccaggtggcc ggcgatctgc cgaaagtgcc gcccccggcg
     4381 ctgctggggt tcctgatggg cggggtggcg gtgcccaacc tggtggcggg tctgttggaa
     4441 cggcagaccg gcggggggct gaaagagttg cccctgcggc ttatcacgtc agggttgttg
     4501 tccgatgagt cggtggaccg gcgggtggag tgggccctgg cggcgttcgc caaacggagg
     4561 cggccgtgag gcggttcgtc ggcgcggcgc tgtgcggcgt cctcgcttgg gggttcccgg
     4621 ccggcccggc gggcgcccag gactcgctgc ggctcggttt gcgggagacc gaggaacggg
     4681 cgctgaagaa ttccgagtcg ttgcgggcgg cggaacggga actggccgcg gcccgcgccc
     4741 gggaacgcgg ccagcgcgcg ccgcttttcc cccaggtttc tctcgacggt tccttccggt
     4801 acgtcagcga ggtggctgcg ttccggccgg cggccggcgg gccggaggtg aaactggggg
     4861 acaacgagaa ctattccgtg ggcccgacgt tatcgtggtt gatgtgggat tcgggcggtt
     4921 cgcggaaccg ccggcgcagc gcgtcggcgc gtcggcgcgc caaggaaaac gaggccgagg
     4981 cggcgcggcg caatgtgttg ttggaggccc ggctggacta ttttcaagtg cagttggccc
     5041 tggaacaggt gcggcttttg gccgactcgc tggcgctggc cgaggcgcaa tataaggata
     5101 tatcgctgaa acagaaggcg ggggcggtga gccggctgga cgcgttgtcg gcccatcagg
     5161 aggtgttgtc gcgtcaacgg cagttgcggc aggcccagac ggagttgtcg gtggcgttgc
     5221 ggacgctcct ggcccgcacc cggcccgaaa cgccggtgga cccgtcggtg acgggccccg
     5281 tggacgggtt gggccggtgg ccgtcgctgg cggaaacgcc cacgctgttg ttgggattgg
     5341 aggatttgtc ggtcacccgc ggccggctgg agccggcggt ggcggcggcg ccggacacgg
     5401 agcatcccca acggatggcg ctgttgcaaa cggcggagtc agcgcgccgg gcggcccggg
     5461 cgttggcggg cggcggcggg ccggtgcttc agttctccgc ccggaccagc ctcgattatc
     5521 ccaacggtcc gaagctggag accattcacc agaacaccgt cggactgagc gcccggcttc
     5581 cgctgtttga ggcggggaaa tcgaaacgcg aggccgagga acagaacgag caggccgcgg
     5641 ccctggaggc ccggcgcgac gaggccgctg tggaactgga ccgcgcctgg cggcaggcgc
     5701 acgaccagat caagggattg accgcccagc tggagatcgc gcggcgctcc gtggacgaaa
     5761 cggaggagct ggcccggctc acctacgatt cctacacggc gggccggtcc agtttcatcg
     5821 aggtgcagtc ggccaacctg cgggccctgg aggcgaaggt ggtggcggcc cggaccgagg
     5881 tgcagatttt gatgcagctt gcgatcgtgg acggattgtc caggaaggga tgaggccatg
     5941 aaaaagaaaa ttttgatcgg actgctggcc gtggcggcgg ggatcgcggc gtggcggctt
     6001 tcgagcccgc gcgaattttt ctacgcgggc acggtggagg ccacggaggt ggacgtgtcg
     6061 gcgcgggtgg cgtcgccggt ggcggcctac gaggtgttcg agggggccga ggtgtcggag
     6121 gggcagaccc tggtgcgtct ttccggcgag gacatccggc tggccaagga acagaccgag
     6181 cgcgacttcc agcgggcgaa gaagttgtac gagggcggga gcgtgtctca ggaggttttc
     6241 gaccggctgc ggttcctgcg ggacgacgcg gccctgcggg ccggctggtg cgaggtccgt
     6301 tcgccgctgg ccgggacggt gttgcgcgtc taccacgagg cgggggagca ggcgatgccg
     6361 gggaccaaac tgctcaccct ggcggacctg cgcgaggtgt gggcgtacgt gtacgtgccc
     6421 cagccgctgt tggcgcggat ctccctcggc caggaggtgg agggactgct gccggaaacg
     6481 ggccgcgcgc tgaaagggac catccggttc atccgctcgg aggcggagtt cacgcccaag
     6541 aacgtgcaga cgcgcgagga gcgcacccgg ctggtgtacg gcgtgaaggt ggtgttcccc
     6601 aacccggagc gggtcctgaa gcccgggatg acggtggaag tgcggctgcc gcgggggaaa
     6661 tgagccgtcg gagaaaccat gttcctcggc gtcgaggtcc gcgaccttca taagaagctg
     6721 ggcgcccagc gggcgctggc ggggttttcg tcggattttt ccgccggcgc gctgcacggc
     6781 gtgatcggcc cggacggcgc gggcaagacc acgctgctcc gcctgctggc cgggctgctg
     6841 cgtcccgacg ccggcgagat cgtgttcacc gacggcggcc ggcccgcgcc gttcgcgtcg
     6901 gtaaggccca cgctggccta tatgccgcaa caacagagcc tctatcccga cctcacctgc
     6961 tcggaacacc tcgatttttt ccgcgacctc tatcgcctgc cgaaagacgt gtaccgcgaa
     7021 cggagaggga aactcctgca tctgacgcgg ctggaaccgt tccagtcgcg ccgggcgggg
     7081 cagctctccg gcgggatgta caaaaaactc ggactgatgt gcgcgctgct ccagtcgccc
     7141 tcggcggtgc tgctggacga gccgaccaac ggcgtggacc cggtgagccg gcgggagttc
     7201 tgggaactgt tgtacgggct ggtggaacgg aagaccctgg tggtggtgag caccgcgtac
     7261 atggacgaag ccgagcgctg cggcgtggtg catctgatgg acgccggccg gcggatctct
     7321 tccggcgagc cgcgcgccct gctggaaaaa gagggcgcct cggggttcga tgagatcttt
     7381 cgccggcggg cggcggaggc gcggcgatga acgggccggg cgtggcggtg gaggtttccg
     7441 gcctgacggt gaggttcggc gatttcaccg cggtggacga cgtttctttc cgcgtggcgc
     7501 ggggagagat cttcgggttc ctgggcgcca acggggccgg caagaccacc accatccgga
     7561 cgctttgcgg cctgctggcg ccgagcgccg gccgggtgcg cgtggccggg ctggggtttg
     7621 aggacggcgg ccgggccatc aaggcccgcg tgggatacat gtcgcagcgc ttcacgctgt
     7681 acaacgattt gaccgtgcgg gagaacctgg cttttgccgc ggggctccgg aagatggagg
     7741 agggggttct ggcgcgccgc acgcgggaac tcttcgcgtt cgtcggcttc gaccggccga
     7801 tgacggaact ggcgcgggac ctgccgggcg gcgtgaaaca gaagctggcg ttggcggtga
     7861 ccctgctgca cgagccggag atcctttttc tg
//
LOCUS       DAOTFD010000113         7848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6554_length_7848_cov_4.514179, whole genome shotgun sequence.
ACCESSION   DAOTFD010000113 DAOTFD010000000
VERSION     DAOTFD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6554_length_7848_cov_4.514179"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="P5079_07275"
     CDS             <1..483
                     /locus_tag="P5079_07275"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRY29824.1"
                     /translation="ALATITWKIVLPGTVASTMGAALCYYIALWGGKVFVERFHGYLG
                     FSWGDIERMSHRMRTSGVAATIFFMRALPIVPLSLVSMVCGVADVPSRTFLVWTVLGT
                     IPRCYILGILGWQLGSSAMAWARGVNRFESVISLTIVLAVFGGIFYLRRRLRKDVQEG
                     "
     gene            complement(491..2236)
                     /locus_tag="P5079_07280"
     CDS             complement(491..2236)
                     /locus_tag="P5079_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRY29825.1"
                     /translation="MDEKSTQKKGVAVMELAMEMALTDDILDKIAAKHGPRMACDAAM
                     STSVGGIGPLLRERVIAQAEKGVNVVGVSLLYETVWRQGLHNWNHLFLERRTVGDAIR
                     SVLTDTGITLTLALYDKTQVTVKVWKTAYGEAPVYFLDCPDIANVVYPCQEDSPEKTP
                     DPTWWADQQRLKQSWLVGRGALALAKTLNFKPDVIVLSETPTLFVHHRLIKDEFQKDP
                     LFDGTRYIFNDHTPLEYAHPIWPIHTLRQLNMDPEIYSPFLAGSGDHAHVDITRLLVA
                     TAEGVFGVAKKHGVVMRAMPSLKDYASKINSITNGVSEAIWQNKVFSFADKLSDADLL
                     AAKEKLKESFIGWLWRRAGLWPVWVRVARKKAFLLWTRRITSYKRLDILQRVLLDPQM
                     RKRFLATDIVLIVGGRIYQRDNVSEKMVYGLVELLNEDQELGDRIIFLDNYNVWEAPK
                     LFHAADGAIMLADDGREASATGFMKAQMNGGLIIANSDGAVPEFVHFLDQSPADKTNG
                     FGVSYVNGQPDPGSFMAALENFDAVYKNPNRRAAMMRSALVVTPQVSVRRTVDDTIAF
                     YNQVIAGGRKVPATK"
     gene            2379..3797
                     /locus_tag="P5079_07285"
     CDS             2379..3797
                     /locus_tag="P5079_07285"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRY29826.1"
                     /translation="MKTRVEKDSLGSRNVPAEAYYGIQTLRAMENFPVSGWRFPRGFI
                     AALGLVKKAAAESNADMGLLDEKTGRAVARAAGELAEGKFDADFPVDIFQTGSGTSAN
                     MNANEIVAHRANELLGGAKGAKSPVHPNDHVNMGQSSNDVIPTALHVAALRLLRQDLL
                     PALEALWKSLEKKQREFDGIVKTGRTHLQDAVPVRLGQEFGGYAAQMRQAIRRLKNCE
                     LPLAELAFGGTAVGTGLNADPAFVSAVVEKISHATGLPFVGAANRFEALAARDAAVET
                     SGALRSLAVSLMKIANDLRWLSSGPRCGLGEIELPALQPGSSIMPGKVNPVIPESAAM
                     VAAHVIGADAAVAVAGQSGNFELNVMQPLIVYHLLTSLELLGNAARLLAEKCVAGIKA
                     NRAVCEAYVEKSPAMATALAGRIGYDKAAALAKEALRTGKTVRQLALEKKLFSKAELD
                     EILNGRRLTEPGIPGKKKTRGK"
     gene            3860..5437
                     /locus_tag="P5079_07290"
     CDS             3860..5437
                     /locus_tag="P5079_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29827.1"
                     /translation="MVAGAFFGAAALFPYLYGYLHQDAGWVFTGIVRLVGDNASYLAW
                     AKQAQEGFALFAVPYTTEPQPRVLFLPFFLLVGWAARLTGLALPLCMNLFYAAGIGFF
                     LWALWRWLKATGASPAVRGLAFFLALFSSGFGAFLDRVAAEFPGLSPLARFPTGTPGI
                     YGAADLWSFDFITFANVAHYPVAPVSLGLLLLSLAWMSRGEEKNNPWAAGVTAGVLFL
                     VHPYDAAIAWGLVWMFPFVQRIPLKRAVSALMIFYGAALPAAVYLLWMMGANPLLREY
                     SRSLHALTPSLVSYLVGLGPTFLLALAGAWCIYRRGERGGIPTLVWALAALGALGLPL
                     TARFKLMHGAHIAFCFLAARGVAAVMEWTGTRAMGKTAALIGIVLLFGAGGTSNARLW
                     KENMENVSRHWLPYYLPRPLEEVLGHLDRNAEPYKTVLTSVNLGLFIPAWTGCKTYAG
                     HWDQTLHREFKEREIADFFSSEASVESRWEFLKTHGISYLLLPLAHRGAMTGRGGEEK
                     MFEKEFDNGYYHVYKVR"
     gene            5418..>5674
                     /locus_tag="P5079_07295"
     CDS             5418..>5674
                     /locus_tag="P5079_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29828.1"
                     /translation="MSTKSVDRLALIGILAAGTAALGLLKFFPDGYFHDDAIYWLLSG
                     SLAKGHYVLPNDPAGGAVVNYLPGYPLALLLVRWTGHFTAAR"
     assembly_gap    5675..5684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5685..7268
                     /locus_tag="P5079_07300"
     CDS             <5685..7268
                     /locus_tag="P5079_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29829.1"
                     /translation="RLLSWASTVLAGFLAWRIFRSAFGPKTGLWALAWFVFQPISVRY
                     AGTVLTEPLWDLLMLVCFWIHWEGPRDSRWADAALALSAGYACLVRPTGALLVAALML
                     DAFSRGSFRRAAYGVAAVAVFQGGLSLWTTVHGQGASSFLGQWRFALQGAPISAFVST
                     VGENLKYYRDIVVFMGVFPLGKWSAVMEGTFVKLPLTVAVWGAVLWGAWRVFQSRHRA
                     LAVFFFLNALLMAAWLRPDPRYLMPALFGILFFMVAAVARRPALGEPPELDRRWKRAV
                     CLAGIVLAVLTTGRGVVSAFRGAGPRLPQETLAWIEDHLPSDAGLAGSFASTVYLHVG
                     CRGTDFPRTYVPEDFLSVLLDRGMTHLLVDEGGTTLAALPDVNDWTPVARWMATHPGW
                     YRSLARIPGDKKIVYAIAVDRETYRKAYDLYQEGLRAMKDNRFDLAETYFQKTVETLP
                     DMPGAADRLAQLAFMRGDTRAAEKLLLESLKTWPENILARHHLFQLRGGNDPAEEAEL
                     RKLSRRHSYWPLLRPYSKK"
     gene            7375..>7848
                     /locus_tag="P5079_07305"
     CDS             7375..>7848
                     /locus_tag="P5079_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRY29830.1"
                     /translation="MAGETFLSRQGYEKLRQDLAGLKERRQNLAREIGEAAEKGDLRE
                     NAEYHAAKEEQQKVVRRIDDLEAKLRNSRIIEEMNVQAGEVRIGCSVRIREAKSGEEF
                     KYTLVDATEANGCLWIMPGSHRNPVVRHRLHESGKYLEIAKEDLPQGEAVACPVPK"
BASE COUNT         1336 a         2185 c         2626 g         1691 t
ORIGIN      
        1 gccctcgcca ccatcacctg gaagatcgtg ctgcccggga ccgtcgcctc gacgatgggc
       61 gcggcgctgt gttattacat cgctctgtgg ggcggcaaag tcttcgtcga gcggttccat
      121 gggtacctgg gtttttcctg gggagacatt gagcggatga gccaccgcat gaggacctcg
      181 ggcgtggcgg ccacgatctt tttcatgcgc gccctgccca tcgtgccgct gtctttggtg
      241 tccatggtct gcggggtggc ggacgtgccg tcgcggacat tcctcgtctg gacggtcttg
      301 gggacgatcc cccgctgtta catcctgggc attctggggt ggcagttggg atcgagcgcc
      361 atggcttggg ccaggggcgt gaaccggttc gaatccgtga tttcgttgac gatcgtgctg
      421 gcggtgttcg gcgggatctt ttatcttcgt cggcgtctgc ggaaggacgt ccaggaaggg
      481 tagggcggaa ctacttcgtc gcggggacct tgcggcctcc ggcgatgacc tggttgtaaa
      541 aggcgatcgt atcgtcgacg gtgcggcgca cgctcacctg cggagtcacc accagggctg
      601 accgcatcat ggcggcccgc cggttcggat ttttgtagac cgcgtcgaag ttctccaacg
      661 ccgccatgaa ggaaccggga tcgggctgtc cgttcacgta ggaaacaccg aacccgttgg
      721 ttttgtccgc cggcgactgg tccaggaagt ggacgaactc cggcacggcg ccgtccgagt
      781 tggcgatgat gagcccgccg ttcatctggg ccttcatgaa cccggtggcc gaagcctccc
      841 ggccgtcatc ggcgagcatg atggcgccgt ccgcggcgtg gaacagtttc ggcgcctccc
      901 acacgttgta attgtccagg aagatgattc ggtctcccag ttcctggtcc tcgttcagca
      961 gttccaccag tccatacacc atcttttccg aaacattgtc ccgctgatag atgcgtcctc
     1021 cgacgatgag aacgatgtcc gtggccagga aacgtttgcg catctgcggg tcgaggagca
     1081 cccgttggag gatgtcgagt cgtttgtagc tggtgatgcg ccgcgtccag aggaggaacg
     1141 cttttttgcg ggccacgcgc acccacaccg gccacagccc ggcccggcgc cacagccagc
     1201 cgatgaagga ctccttgagt ttctccttgg cggcgaggag gtcggcgtcg gaaagtttgt
     1261 cggcgaagga aaacaccttg ttctgccaga tggcctcgct gacgccgttg gtgatggagt
     1321 ttatcttgga ggcgtagtcc ttcagggacg gcatggcccg catgaccact ccgtgttttt
     1381 tcgccacgcc gaagacccct tccgccgtgg ccaccaacag gcgggtgatg tccacgtggg
     1441 catggtcgcc cgatccggcg aggaacggcg aatagatttc cgggtccatg ttcaactgcc
     1501 gcagggtgtg gatgggccag atgggatggg cgtattccag aggcgtgtgg tcgttgaaga
     1561 tgtagcgggt gccgtcgaag agcgggtctt tttggaattc gtccttgatc aggcggtggt
     1621 ggacgaaaag ggtgggcgtt tcactcagga cgatcacgtc cggtttgaaa ttgagcgttt
     1681 tggccagggc cagcgcgccc cgtcccacca gccagctttg tttcaggcgt tgttggtcgg
     1741 cccaccaggt cgggtccggt gttttctcgg gggagtcctc ttggcaggga tagaccacgt
     1801 tggcgatgtc ggggcaatcg agaaaataga cgggggcctc cccgtacgcg gttttccaga
     1861 ccttcacggt cacctgggtc ttgtcgtaga gggcgagggt gagggtgatc ccggtgtccg
     1921 tcagaacgct ccggatggcg tccccgacgg tccgccgttc gaggaacagg tggttccaat
     1981 tgtgcagccc ttggcgccac acggtctcgt acaggaggga aactccgacc acgttgacgc
     2041 ccttttcggc ctgggcgatg acgcgttctc gcaggagagg accgatgccg ccgaccgacg
     2101 tggacatggc cgcgtcgcag gccatccgag gaccgtgctt ggcggcgatt ttgtcgagga
     2161 tgtcgtccgt gagggccatc tccatggcca actccatcac ggcgacgcct tttttctggg
     2221 tggatttttc gtccatgaga aaagcatcat actatacccg cgctcggtct gtcaaaaaaa
     2281 tgaagggatc aagtgggggc ggacgcgtcg ggcggcggag aaattgataa aatgggatgc
     2341 gccggtgcgg ttcgtgacgg cgaataaaag gagtccctat gaagacacgc gtcgaaaagg
     2401 attctctggg cagcaggaac gtgcccgcgg aggcgtacta cggtattcaa acgctccgcg
     2461 ccatggaaaa ttttccggtg agcggctggc ggttcccgcg cgggttcatc gccgcgctgg
     2521 ggctcgtcaa gaaggccgcc gccgagagca acgcggacat gggactgctc gatgaaaaaa
     2581 cgggccgggc cgtggcccgc gccgccgggg aactggccga ggggaagttc gacgccgatt
     2641 tccccgtgga catttttcag acgggatccg gcacctccgc caacatgaac gccaacgaga
     2701 tcgtcgccca ccgggccaac gagctcctgg ggggagcgaa gggagcgaag tctccggtac
     2761 atcccaacga ccacgtgaac atgggacagt cttccaacga cgtcatcccc accgccctcc
     2821 atgtggcggc gttgcggttg ttgcggcagg acctgttgcc ggcgctggaa gccctgtgga
     2881 aatcgctgga gaagaagcag cgggagttcg acggcatcgt caagacgggc cgaacgcacc
     2941 tccaggacgc ggtgccggtg cgcttggggc aggagttcgg cggctacgcg gcgcagatga
     3001 gacaggccat tcgacggctg aagaactgcg agctgccgct ggcggaattg gcgttcggcg
     3061 gcaccgccgt gggaacggga ttgaacgccg atccggcgtt cgtgagcgcg gtcgtcgaaa
     3121 aaatttcgca cgcaacgggg cttccgtttg tgggcgcggc gaaccgtttc gaggcgctgg
     3181 ccgctcggga cgccgccgtg gagaccagcg gcgctctgcg ttccctggcc gtgagcctga
     3241 tgaagatcgc caacgacttg cgctggttgt cgtcgggtcc tcgctgcggc ctgggggaga
     3301 tcgaactgcc cgcgcttcag cccgggagtt ccatcatgcc ggggaaagtg aaccccgtga
     3361 ttccggagtc ggccgccatg gtggcggcgc acgtgatcgg cgcggacgcg gccgtggcgg
     3421 tggcgggcca gtccggcaat ttcgagctga acgtcatgca gccgctcatc gtttatcatt
     3481 tgctcacttc cctggaactt ctcgggaacg cggcgcgcct tctggccgaa aaatgcgtgg
     3541 cgggcataaa ggccaatcgt gccgtgtgtg aggcctatgt ggagaaaagt ccggccatgg
     3601 ccacggccct ggccgggcgc atcggctacg acaaggcggc cgcgctcgcc aaggaagccc
     3661 ttcggaccgg caaaacggtc cggcaactcg ccctggaaaa gaaacttttc tccaaagcgg
     3721 agttggacga gatcctcaat gggcgccgtt tgacggaacc gggcatcccc gggaagaaaa
     3781 agacgcgggg aaaataaatt ttcaccggcc gccgatgcag gcctcggtaa aacggttact
     3841 ggcacatccg cggtttcctt tggtcgccgg cgcctttttc ggggcggcgg cgctcttccc
     3901 ctacctgtac ggatatctcc atcaagacgc gggatgggtg ttcaccggca tcgtccgtct
     3961 cgtcggagac aacgcctcct atctggcctg ggccaagcag gcccaggagg gtttcgccct
     4021 cttcgccgtg ccgtacacca cggaacccca gccccgcgtc ctcttcctgc cttttttctt
     4081 gctggtcggc tgggcggccc gactgaccgg cctcgctttg ccgttgtgca tgaacctttt
     4141 ttatgcggcg gggatcggtt ttttcctgtg ggctttgtgg cggtggttga aggcgacggg
     4201 tgcttctccg gccgtgaggg ggctggcctt ttttctggcg cttttttcca gcggttttgg
     4261 cgccttcctg gatcgcgttg cagcggaatt tcccggcctg tctccgctgg cgcggtttcc
     4321 gacgggaacg cccggcattt acggtgcggc cgacctgtgg tctttcgact ttatcacctt
     4381 cgctaacgtg gcgcattatc ccgtggcgcc ggtctctctg gggttgctgc ttctgtcgtt
     4441 ggcgtggatg agccgagggg aggaaaagaa caatccctgg gcggccgggg tgacggccgg
     4501 agtccttttt ctggtgcatc cgtacgacgc cgccatcgcc tggggcctcg tgtggatgtt
     4561 tccgttcgtt cagcggattc cgttgaaacg cgcggtttcg gccctgatga ttttttatgg
     4621 ggccgctctc ccggccgcgg tctatctcct ttggatgatg ggcgccaacc cgcttttgcg
     4681 cgaatactca cgctcgttgc atgcattgac cccgtcgctg gtttcttatc tcgtcggttt
     4741 gggtccgacc ttccttctcg cgttggcggg cgcgtggtgc atttatcgtc ggggggaacg
     4801 gggtggaatc ccgacgttgg tgtgggcgtt ggcggcgttg ggagcgctcg gactgccgtt
     4861 gacggcgcgt tttaaattga tgcatggcgc gcacatcgct ttttgcttcc tggccgcgcg
     4921 gggagtggcg gcggtcatgg aatggacagg gacgcgggcg atgggaaaaa ccgcggccct
     4981 catcggaatc gttctcctgt ttggagcggg cggaaccagc aatgcgcggc tttggaagga
     5041 aaatatggaa aacgtgtctc gccattggct cccctactac ctgccgcgtc cgcttgaaga
     5101 agtcctgggg catttagacc ggaacgccga gccatacaag acggtgttga cgtccgtcaa
     5161 cctcggtctt ttcatacccg catggacggg atgcaagacg tatgcggggc attgggacca
     5221 gacgttgcat cgcgaattca aggaacggga gattgcggat ttcttttcct cggaggcatc
     5281 ggtcgaaagc cgctgggaat tcttaaaaac gcatgggatc tcctatctcc ttctgccgct
     5341 ggcgcatcgg ggggcgatga ccggccgggg gggagaggaa aaaatgtttg aaaaagaatt
     5401 cgacaacgga tattaccatg tctacaaagt ccgttgaccg tctcgcgttg atcggcatcc
     5461 tggcggcggg gacggccgct ctgggtctct tgaaattttt ccccgacggc tatttccatg
     5521 acgatgccat ctattggctc ctctccggtt ctttggcgaa aggacattac gtcctgccga
     5581 acgatccggc gggcggggcg gtggtgaact atttgccggg ctatccgctc gcgttgctcc
     5641 tggtgcggtg gacggggcac ttcacggcgg cgcgnnnnnn nnnncggctg ctttcttggg
     5701 catcgacggt gttggcgggt tttttggcgt ggagaatatt tcggagcgcg ttcggtccga
     5761 aaaccggact ctgggccctg gcctggttcg tgtttcagcc gatctccgtt cgctatgccg
     5821 ggaccgtgct caccgagccg ctgtgggatc tgttgatgtt ggtttgtttt tggatccatt
     5881 gggaaggacc gcgcgattcc cggtgggcgg acgcggcgtt ggcgctgtcg gcggggtacg
     5941 cctgtctggt gcggccgacg ggggcgctcc tggtggcggc cttgatgctc gacgcttttt
     6001 cccgcgggtc gttccgccgc gccgcatatg gggtggcggc ggtggcggtg tttcaggggg
     6061 gactttccct gtggaccacg gtccatggac agggagcgag ttctttcctc ggccaatggc
     6121 gattcgcgct tcagggggcg ccgatctcgg cgttcgtttc gaccgtcggc gagaacctga
     6181 aatattatcg agacatcgtt gttttcatgg gggtgtttcc tctcgggaaa tggtcggcgg
     6241 tcatggaggg gacattcgtc aaattaccgt tgacggtggc cgtttgggga gcggtcttgt
     6301 ggggtgcctg gcgtgttttt caaagtcgcc accgcgccct ggccgtcttt ttctttttga
     6361 acgccctgtt gatggcggct tggctccggc cagacccgag gtatctgatg ccggcgctgt
     6421 tcgggatcct gtttttcatg gttgccgccg tggcccgtcg tccggcgttg ggagaaccgc
     6481 cggaattgga tcgacgatgg aagagggcgg tttgtctggc gggcatcgtt ttggcggtgc
     6541 tgacgaccgg ccgcggcgtc gtgtccgctt ttcggggggc cggaccgcga ttgccgcagg
     6601 aaacattggc ttggatagag gaccatttac cgtccgacgc tgggctggcc ggttctttcg
     6661 ccagcacggt ttatctgcac gtcggctgtc gcggcacgga tttcccacgc acctacgtcc
     6721 cggaagattt tctatcggtc ctgttggacc gtgggatgac tcatttgttg gtggacgagg
     6781 ggggaacgac gttggccgcg ctgcccgacg tgaacgattg gacgcccgtg gcccggtgga
     6841 tggcgacgca tcccggctgg tacaggtcgc tggcacgtat accgggcgac aagaaaatcg
     6901 tttacgccat cgcggtggac cgggaaacct atcgcaaagc ctacgatctc taccaggaag
     6961 ggcttcgggc catgaaagac aatcggtttg atcttgcgga aacgtatttt caaaaaaccg
     7021 tggaaaccct gcccgatatg cccggtgccg ccgaccggct cgctcagctt gcgttcatgc
     7081 ggggggacac gcgggcggcg gaaaaattgc tcctggaaag cctgaaaacc tggccggaaa
     7141 atattttggc gcgccatcac ctcttccaac tgcgcggcgg gaacgacccc gcggaggagg
     7201 ccgagttgag gaagctttcc cggcgccatt cctactggcc cctgctccgg ccgtactcaa
     7261 agaaataatc ccgctttttc caaaaaagca aataaacgag ttgtttttag gggatttgct
     7321 ataataaatc cttcgtatag ttactattac taatagataa caatggaggt tgccgtggca
     7381 ggggaaacgt ttttgtcgcg acaggggtat gaaaaattac gccaggattt ggcgggcttg
     7441 aaggaacgac gccagaattt ggcccgcgag atcggcgagg cggcggaaaa gggcgatctg
     7501 cgggaaaacg cggagtatca cgcggccaag gaggaacagc agaaggtggt ccgccgcatc
     7561 gacgacctgg aggccaaact gcgcaactcc cgcatcatcg aggaaatgaa cgtgcaggcc
     7621 ggcgaggttc gtatcggctg ttcggtgcgc atccgggagg cgaagagcgg ggaggaattc
     7681 aaatataccc tggtggatgc gacggaggcc aacggctgct tgtggatcat gccgggttcg
     7741 catcggaatc cggtggtgcg gcatcggctg cacgagagtg gcaagtacct cgagattgcc
     7801 aaagaggact tgccgcaggg cgaggccgtt gcatgtcccg ttcccaag
//
LOCUS       DAOTFD010000114         7811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6592_length_7811_cov_4.435534, whole genome shotgun sequence.
ACCESSION   DAOTFD010000114 DAOTFD010000000
VERSION     DAOTFD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_7811_cov_4.435534"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>7811
                     /locus_tag="P5079_07310"
     CDS             46..>7811
                     /locus_tag="P5079_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29831.1"
                     /translation="MTAGKAAGTVVEVGKAISVGTRLMNAVKIGLFMGAFNAGNLLLQ
                     SISSGHAPSVTQLAAAFGVGFLMGMVFGSVSSRVSSAWVSNIRSQFVNYLRNPLTFMA
                     SAVNTAGSLAIFNATPFGAAFNYVMAIAAGKDFGWAQYFGSISQGMQFGLTMGPFFAM
                     AMPAQAISRGELMTAFTEIGPTRTLLRSLFGGSVTDALAWTPLAARGGLMGQIGQWLH
                     MTEMVGVYHIASVVGGAIGGLFDRLLGTTNFMDSPEGPVGLFASTLANLSFFLVPTAM
                     ATRTEVTTKDTVSYLEGGLKKLETSRTVLEAEGEGNLLVVENGSARIVKPGEADYAAC
                     RSELAKAERPTTGTEAAEAGRTEAAREMRPAEEMKTPETGKVEGAAGERASLPEMSRP
                     ETSLEITASRPEAAKLREAELPAGVREVAAKAKAEAAPRARVVVEITEGLKFLAGRTV
                     REAMTPAKVNEVLAKRAGEVVEIGGTLFTVDRGLTTLAKFAKMTAGPNGEEVMRGILT
                     GEIKEGRMMNEVTVTRGLADMARAEMTVSQSPLRAFLSSSLESGAVKRTEEGYELKVS
                     RESLPKELRDILPEKGLGEIRLEIDARTGELKLMADIQGVETVGQFLENFYKQPGNDE
                     SGHGAESAKKAIEELKTQLVDGVTFKAELALDAQGRIREGTLTARGEFNKIVKENAPI
                     REGKGGQLAAKYNLTLEVKIQFKAGGEVKLTYRVIADQEKMNDANFCKDLKEALGKET
                     AERLDALVGGEGNLGKIRELSFEVDAKGNVVKDSLRVVMDHADVQLVEGALVAGTLGF
                     LRLARSEGGNVIDIEGPVTLRQGSEGKFELESIKFAVKEGKSLNDLIKGLLGKRQKPG
                     VDLEMGILLEELLRQTGLDLNSAKFELFRGRDGEKKLYVEVNGKILEAYSVVGRAAGV
                     RLADIRETQQSLDLVRLELREANVLDRGRLESKSRYLEARLDALYGRKSLESWSLHRM
                     EQRWMSREADREISARRTQLEDKSLAPEKKAAVAREMQVWEARRSELSKWRVLLGSRS
                     AKTAYEIGTFKATRAQVRAECDKKIGELRTTIREARGKGDFVSVRRAEADLKAERERR
                     SGLRRLSWKEADAMRVAMEQTTLLSRLPLDKLEAARRQELQEGMTKTDVERYEQELAA
                     AEKAGDRRRVELLRDKIEMSRAMQELMELALEPSRAAENPAEYFGAIRQAIDIISGVI
                     TRRMEARGLKADIFADLKSQDRQVHNRAMEKLLKEVFGIEGGDTTKLNFAQRFAIEFK
                     VMESVYGKNPLFLGKFPLQAVLFVNCLVGRIGSLKTAGGKSAVFTMVFGDMALRGKLR
                     GKVGELIVDKYAEIEKYTDKDYPPLAAAFGVKYVDGAKYYHTRNYKELSEIYRSAEFE
                     GESGVVMVYDSHSRGFLELTARADNPFDLNSTLNNVSARGVDEADVPALSRLAFIESR
                     SNVRATEAEVQNVEGLLRNFETHLGLTDALLKRLDGQGAKALEGLKVEGAEGTRYVDR
                     EAFEALRYSEEKVFTVYDGKIQASKGLIEALRGPAEAKTRYSTQQVENALRALYDIAT
                     HQDAKVDILTGEIVRYDEKGVMQKGVIDGSLTYNISMTLLKIRSLDAQGVQHGVDRLN
                     VRISDAHAEATLSQIFSRSADGQLLTFGGSATLDVAGEIARVIFDALPIEIEGSDFVK
                     DYLTNPSKLAPEIKDVSDPSAQVSEALSLIRKAVAEKQGALIGVGDVMKQKMLLAEIL
                     IKIGGESSEAVAKLLSDTVGKSMSEVLLEARARGMKIAERIDVIDGSLTPYKSPEQIA
                     ESSGRIVKDGKVIQEGRIVISNLSGLRAISYKGEVHMAIIDAHRMTYGELLQALGRVE
                     RSDFTAKSERLIMIDSREMGFVLREMIKADTYFRQKGVADGLFEGRKATREKFEAFIK
                     GVTPESLAKGLDRLGKPLTREQLRDLVQVATEFRTLLEKSESIQFKSSVETLTKILKE
                     PLARMRALDRDFVSGLEREILSKSEDFSSEMSAGEFRDPIRMGKDGFLQAANFAISMW
                     ARVAAESGSKVARAEAKLRISEIQRAIDQVDATFANAERVERTFASTPESVYGRPAQH
                     VAGVLASLSRFIMPVEGRGFAQTQALAKAPKTVLEAQAALKEMGIQEGTPEFARVTDS
                     LQARPADQKGVPPLSYGDGTLTFAGKALMLSIQQFGGLSEEDRTAVTGLVTGVLGVAV
                     TLPSEATAWAAEAAMSLYGAGVLPLENFTGNTVNEMVGYGAMAGLLAKMGAPVGADQL
                     KINLRSSNPYGEFAKAIAFAGKTAGKEYRGVVKYFRDMVLYGERLTAAQRLAPGKGFK
                     WVVQLPAKIGLGWNELRMTSRVSGSQLFKVALGLQARGLADMPAVRALAEMSFSLWTG
                     RATRQEKSSPKITTAELVRNLRAVTGMLADLKTPAAVLALRGRITLKDMWEAPVRQKG
                     FAKIKGMLDQVKGALGKAKPAEVPDYTDSARRISYLSAKLSQTPFTMLQKSALGMATY
                     WGAHFALGLVTGGASTLLTLGTVALSFALNWGINRIALWWTVSRRLGESQIQSQAALL
                     EKAAGRMQPIIERTSKDKSFQSVVDHFYADFSAVKVKYDTLTGSTVAPELA"
BASE COUNT         1635 a         1946 c         2771 g         1459 t
ORIGIN      
        1 aaacttgcgc gtttgggcgc gtttgggaac gaaatccggc tggccgtgac cgcggggaaa
       61 gcggccggga ccgtggtcga ggtgggcaaa gccatttcgg tgggaacgcg tcttatgaat
      121 gccgtcaaga tcggcttgtt catgggcgct tttaacgcgg ggaacctctt gttgcagagt
      181 atttccagcg ggcacgctcc ttctgttacc cagttggcgg cggcgttcgg agtggggttc
      241 ttgatgggca tggtgttcgg gtcggtttcc tcgcgggtgt cctcggcgtg ggtttccaat
      301 atccgaagtc agttcgtcaa ctatctgcgc aacccgctta cctttatggc cagcgcggtg
      361 aacaccgcgg ggtccttggc catcttcaac gccacgcctt tcggggccgc gttcaattac
      421 gtgatggcca tcgcggccgg caaggatttc ggatgggcgc aatatttcgg cagcatttcc
      481 caaggcatgc agttcggttt gacgatgggt ccgttcttcg ccatggcgat gccggcgcag
      541 gccatcagcc ggggagaact gatgacggct ttcacggaaa tcggcccgac ccggaccctc
      601 ctgagatccc tctttggcgg gagcgttacc gacgccttgg cgtggacgcc gttggcggcc
      661 cgaggcgggt tgatggggca aatcggccag tggctgcaca tgacggaaat ggtcggcgtt
      721 tatcatatcg ccagcgtggt gggcggcgcc atcggcggcc tgttcgatcg tcttttgggg
      781 accacgaatt ttatggattc tcccgagggc cccgtcggtc ttttcgcctc gaccctggcc
      841 aacctgtcgt tcttccttgt tccgaccgcc atggcgacgc gcaccgaagt gacgaccaag
      901 gacacggtga gctacttgga gggcggttta aagaaattgg aaacttcgcg gacggtcttg
      961 gaggccgagg gcgaagggaa cctccttgtg gttgaaaacg gttctgcgcg gattgtgaaa
     1021 cccggcgagg cggattacgc ggcttgtcgg agcgaactgg cgaaggccga gcggccaacg
     1081 acggggaccg aggccgccga ggcgggcagg acggaggccg cccgggaaat gcgtccggcg
     1141 gaagagatga agacgccgga aacggggaaa gtggaagggg cggcggggga acgggcgtcc
     1201 ttgcctgaaa tgtcccggcc ggagacttcg cttgagatca ccgcttcccg gccggaagcc
     1261 gcgaagctcc gggaggcgga attgcccgcc ggggtgcggg aagtggcggc caaggcgaaa
     1321 gccgaggcgg cgccgcgggc ccgggtcgtt gtggagatca ccgaaggctt gaaattcttg
     1381 gccggccgga cggtgcggga agccatgacc ccggcgaaag tcaacgaagt gttggcgaag
     1441 cgcgcgggag aggtggtgga gatcggcggc acgctcttta cggtggacag agggctgacc
     1501 acgctggcga agttcgcgaa gatgaccgcc ggtcccaacg gcgaagaggt gatgcgcggc
     1561 attttgacgg gcgagatcaa agagggacgg atgatgaacg aggtgacggt cacccgggga
     1621 ttggcggata tggcccgggc ggagatgacc gtttcgcaaa gccccctgcg cgcgttcctg
     1681 tcgtcctccc tggaatcggg agcggtgaag aggacggaag aggggtatga attaaaagtg
     1741 tcgcgggaga gtctgccgaa agaattgcgg gatatcctgc ctgaaaaggg gctgggcgag
     1801 atacggttgg agatcgacgc ccgcaccggc gaattgaaac tcatggcgga cattcaggga
     1861 gtggagaccg tcgggcagtt cctggagaat ttctataaac agccggggaa cgatgaatcc
     1921 ggtcatgggg cggaatcggc gaagaaagcc attgaggaac tcaaaaccca attggtggac
     1981 ggggtcactt tcaaggcgga actggcattg gatgcgcagg gacgcatacg agagggaacg
     2041 cttacggctc gcggggaatt caacaagatc gtgaaggaaa acgcgccgat ccgcgaagga
     2101 aaaggcggcc aactggcggc caaatacaat ttgacgttgg aagtgaagat ccaattcaag
     2161 gccggcggcg aagtcaagct gacctatcgg gtcatcgccg accaggaaaa gatgaacgac
     2221 gcgaatttct gcaaggactt gaaggaggcg ctcggaaaag agaccgcgga acggctggat
     2281 gcgttggtgg ggggcgaagg gaatctgggc aagatcagag aactttcttt tgaggtggat
     2341 gccaagggca atgtggtgaa ggactccctc cgggtggtta tggaccatgc cgatgttcag
     2401 ttggtggaag gcgcgttggt ggccggaacc ctcggcttcc tgcggctggc ccggtccgag
     2461 ggcggcaacg ttatcgatat cgaagggccg gtcacgctgc gccaagggtc ggagggaaaa
     2521 tttgagttgg aaagcatcaa gttcgccgtc aaagaaggaa aatccctgaa cgacctgatc
     2581 aagggattgc tcggcaaacg ccagaagccg ggggtcgatc tggagatggg gatcctcctg
     2641 gaagagttgc tgcggcagac ggggctggac ttgaacagcg cgaaattcga gctgttccgc
     2701 ggccgggacg gcgagaaaaa gctttacgtg gaggtcaacg ggaagatcct ggaggcgtat
     2761 tccgtcgtcg gtcgggcggc aggggtgcga ttggccgata tccgggagac gcagcagagc
     2821 ctggatctcg ttcgcctgga acttcgtgaa gccaatgtgc tggatcgcgg ccgcctggaa
     2881 tcgaagagcc gctatctgga agcgcggctg gacgcgcttt acggcagaaa atccctggag
     2941 agctggtcgc tccatcggat ggaacagcgg tggatgagcc gggaagcgga ccgggagatc
     3001 tccgcccgca gaacccagct ggaagacaag agtttggccc ctgaaaagaa agccgccgtt
     3061 gcgcgggaaa tgcaggtctg ggaggcgcgg cggagcgaat tgtcgaaatg gcgggttttg
     3121 ctggggtcgc gttccgccaa gacggcgtat gagatcggga cgtttaaggc gacgcgggcc
     3181 caggttcgcg cggaatgcga taagaagata ggggaacttc ggacgacgat ccgcgaggcc
     3241 cggggcaagg gagatttcgt ttccgtccgt cgtgcggagg ccgatctgaa ggccgagcgg
     3301 gaacggcgca gcggtttgag gcgcctgagc tggaaagaag ccgacgccat gcgggtcgcc
     3361 atggaacaga cgaccctcct cagccggctc ccgttggata aactggaggc ggctcgccgg
     3421 caggagttgc aggaggggat gaccaagacg gacgtggaac ggtatgagca ggaactggcc
     3481 gccgcggaga aggcgggcga tcgccgccgg gttgagttgt tgcgtgacaa aatcgagatg
     3541 tcgcgggcca tgcaggaatt gatggaactg gctttggagc cttcccgcgc cgccgaaaat
     3601 cccgcggaat actttggcgc catacgacag gccatcgata tcatctccgg ggtcatcacg
     3661 cgccgcatgg aggcgcgcgg gctcaaggcg gatatttttg cggacttgaa gagccaggat
     3721 cgccaggtgc ataaccgggc gatggaaaaa cttctgaaag aagtcttcgg aatcgaaggc
     3781 ggcgacacga cgaagctgaa cttcgcccag cgattcgcca tcgagttcaa ggtgatggag
     3841 tctgtctacg gcaagaaccc gctcttcctg ggtaagttcc cattgcaagc cgtgttgttc
     3901 gtcaactgtt tggtgggtcg gatcggatcg ttgaagacgg cgggtggaaa atccgcggtt
     3961 ttcaccatgg tgttcggcga tatggccctg aggggcaaac tgcgcggcaa agtgggcgag
     4021 ctgatcgtcg ataaatacgc cgaaatcgag aaatacacgg acaaggacta tccgcctctg
     4081 gcggcggcgt tcggcgtgaa atacgtggac ggtgcgaaat attaccacac gcggaactac
     4141 aaggaacttt ccgagatcta tcgcagcgcc gaattcgagg gcgaatccgg cgtggtgatg
     4201 gtgtatgaca gccattcgcg cgggttcttg gagctgacgg cccgggcgga caatccgttc
     4261 gatttgaatt ccaccctgaa caatgtcagc gcgcgcggtg tggacgaggc tgacgtgccg
     4321 gccctttccc gcttggcctt tatcgagagc cgttccaacg tgagggccac ggaagcggag
     4381 gtgcagaacg tcgaagggct cctgaggaat tttgaaacgc atttgggatt gaccgacgcc
     4441 ctgttgaaac ggttggacgg acaaggcgcc aaggcgttgg agggcttgaa ggtggagggg
     4501 gccgagggaa cgagatatgt ggaccgggag gcctttgaag cgctccgtta cagtgaagaa
     4561 aaggtgttta cggtttatga cggaaaaatc caggcgtcca agggattgat cgaggcgctg
     4621 cggggaccgg cggaagcgaa aacccggtat tccacgcaac aggtggaaaa tgccttgcgg
     4681 gcgctctatg acatcgccac gcatcaggac gccaaagtcg acatccttac gggcgagatc
     4741 gtgcggtacg atgaaaaagg cgtcatgcag aagggcgtca tcgacggctc tttgacatac
     4801 aacatttcca tgaccctcct gaagatccgg tcgctggacg cccaaggggt ccagcatggc
     4861 gtcgaccgcc tgaacgtgcg gatatcggat gcgcacgcgg aagcgacgtt gtctcagatc
     4921 ttcagccggt cggcggacgg gcagctcctg acgttcggcg gttcggcgac cttggacgtg
     4981 gccggggaga tcgcccgcgt catctttgac gcccttccga tcgaaatcga agggtcggac
     5041 tttgtgaagg attatctgac caatccgagc aagcttgcgc cggaaataaa agacgtgagt
     5101 gatccgagcg ctcaggtgtc tgaggcgctg tccctcatcc ggaaagccgt ggcggaaaaa
     5161 cagggcgcct tgatcggcgt gggcgacgtg atgaagcaga agatgttgct ggcggagata
     5221 ttgatcaaaa tcggcgggga aagttcggag gcggtcgcca aattgctttc ggacacggta
     5281 gggaaatcca tgagcgaggt cctgttggag gcccgggcgc gcgggatgaa gatcgctgaa
     5341 cgcatcgatg tgatcgacgg ctcgttgacg ccgtataagt cgccggagca aatcgcggaa
     5401 tcttccgggc gcatcgtaaa agacggcaag gtgatacagg aaggccggat cgttatctcg
     5461 aatttgagcg gcttgcgcgc catcagctat aagggcgaag tgcacatggc catcatcgat
     5521 gcgcatcgga tgacctacgg tgagttgttg caggccctgg gccgtgtgga acggagcgat
     5581 tttacagcca aatccgagcg gctcatcatg atcgattccc gggaaatggg ttttgtgctg
     5641 cgggaaatga tcaaggcgga cacctatttc cggcagaaag gggtggcgga tggtttgttc
     5701 gaagggcgga aagccacccg ggaaaaattc gaggcgttca tcaagggagt gacgcccgaa
     5761 tctctggcca agggattgga ccgtctcggg aaacccttga cccgggaaca gttgcgggat
     5821 ctggtccagg tggcgacgga gttccggacc ctgcttgaaa agtccgaatc catccagttc
     5881 aagtcctcgg tggaaacgtt gaccaagata ttgaaagagc cgttggcccg gatgcgggct
     5941 ttggaccgcg acttcgtttc gggcttggaa cgggaaatcc tgtcgaagag cgaggacttc
     6001 tcgtcggaga tgtcggcggg cgagttccgc gatcccatcc gtatgggcaa ggacgggttc
     6061 ctgcaggcgg ccaatttcgc gatatcgatg tgggcccggg tggcggccga gagcggcagc
     6121 aaggtggcgc gtgcggaggc gaagctgcgc atctcggaga tccagcgggc gatcgatcag
     6181 gtggacgcca cgttcgccaa tgccgaacgg gtggagcgga cattcgcttc cacacccgag
     6241 agcgtttatg gaaggcccgc gcagcacgtg gcgggggtct tggcgtctct gagccggttc
     6301 atcatgccgg tggaaggccg gggattcgct cagacacagg ctctggcgaa ggctccgaaa
     6361 acggttctag aggcgcaggc cgccctgaaa gagatgggaa ttcaggaggg gactccggaa
     6421 ttcgcccgcg tgacggattc tctgcaggcg cggccggccg atcagaaggg ggtgcccccg
     6481 ctctcctacg gagacggaac cctgaccttc gcggggaaag cgttgatgtt gtccattcag
     6541 caatttggcg gtctttccga ggaggaccgg acggcggtga cgggtctcgt gaccggcgtg
     6601 ttgggggtcg cggtgacgtt accgagcgag gcgacggcct gggcggcgga ggcggcgatg
     6661 tctctctatg gggcgggagt cctgccgttg gaaaatttca ccggcaacac ggtgaatgag
     6721 atggtcggtt atggcgcgat ggccggtttg ttggcgaaga tgggagctcc tgttggtgcg
     6781 gatcagttga aaattaatct gagatcgtcg aacccttatg gggagttcgc caaggcgatc
     6841 gctttcgcgg ggaaaacggc cggtaaggaa tatcgaggtg tcgttaagta tttcagggac
     6901 atggtgctct acggggaacg gctgacggcg gcgcagagac tggctccggg caaagggttt
     6961 aagtgggtgg tgcagttgcc ggcgaagatc ggtcttggat ggaatgagtt gaggatgaca
     7021 agccgtgtct ccgggtccca gttgttcaag gtggccctcg gtttgcaggc ccgcgggttg
     7081 gcggacatgc cggcggtgcg ggctttggcg gagatgtcct tctccctctg gacggggcgg
     7141 gccacccggc aggaaaaatc ttcccccaag atcacgacgg cggagctcgt tcggaacctg
     7201 cgcgcggtga cggggatgct ggcggatctg aagacccccg ccgccgtgct ggcgttgcgt
     7261 gggagaatta cactcaagga tatgtgggaa gcgccggtgc gccagaaagg ttttgcgaag
     7321 atcaaaggga tgctggatca ggtgaaaggg gccctgggga aagcgaagcc ggcggaagtt
     7381 ccggattaca ccgattccgc tcgtcggatt tcctatcttt cggcgaagct gagtcagacg
     7441 ccgttcacca tgctgcagaa gagcgcgctg gggatggcga cgtattgggg ggcgcatttc
     7501 gctctggggc tcgtgaccgg cggcgccagc accttgttga cgcttggaac cgtggcgctc
     7561 tcgtttgcgc tgaactgggg catcaaccgg attgcgctct ggtggacggt cagtcgtcgt
     7621 ctgggtgaat ctcaaatcca gagccaggcg gctttgctgg agaaggccgc cggaaggatg
     7681 cagccgataa ttgaaaggac ttcgaaagat aaatcgttcc aaagcgtggt cgaccatttc
     7741 tacgcggatt tctccgcggt gaaggtgaaa tacgacacgt tgacgggctc gacggtggcg
     7801 ccggagctgg c
//
LOCUS       DAOTFD010000115         7802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6603_length_7802_cov_4.991997, whole genome shotgun sequence.
ACCESSION   DAOTFD010000115 DAOTFD010000000
VERSION     DAOTFD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6603_length_7802_cov_4.991997"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5079_07315"
     CDS             complement(<1..535)
                     /locus_tag="P5079_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRY29832.1"
                     /translation="MSAEIVPRFYRVSNDPWVVRLVARLDWGLLGAVLALSLMGLMFI
                     FSATAHGGQSYAYLSRQFTALLVGLAGATLIVFLPYQVLLTYYRWIYLISLAFLIGVL
                     VFGVKLRGSKSWFDLQFIYFQPVELTRLALTVALAAYADFHLRELREWRGLIVPFVLT
                     GLHIGAGRSVFLHGPSGS"
     gene            complement(532..2292)
                     /gene="mrdA"
                     /locus_tag="P5079_07320"
     CDS             complement(532..2292)
                     /gene="mrdA"
                     /locus_tag="P5079_07320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY29833.1"
                     /translation="MTDNRPHVDVLADWRLKAITIFVSACFAVLGLRLFQLQILRGSE
                     MARLAEMNRTQIIPLRAPRAVVMDRHGDVLLENAPNFSLFYSAQALPEKAHRNVEDDV
                     LRWFPAMEPLLKRKFAEARLTGKMTRISADIPRPVALALIEKRLSLPGVSVVVEPKRR
                     ARYGSLCSHVLGYVDEISSAELERLREEGYRMGQLVGRMGIERVYENILRGEDGGIQF
                     EMDAGGRHVHVMRRIAPRPGPLATLTIDRRLQEAVEQGLKASETKKGAAVVVDPRSGA
                     VLALASSPDFDPSASVIPYLTDPGLPLFNRAIQGTYPPGSVFKIVDAAAALFDADWDI
                     KQTFNCPGKFYVGSKEFGCWGVHHVKDFMGAVAWSCNVYFYNLGIRTGPDAIERMARA
                     FGFAEKTGIDLTSETAGFIPGRAWKKEYRKVPWFDGDTANLAIGQGAVAATPLQVAMM
                     VSSVANGGQLWKPFVMDSVVDRSGRVLFKQSPVLRRTVEMPQRIWERLGLAMQGVVTV
                     GTGRGVHRPDLVVGAKSGTAQNPHGREHAWFAAYAGRPGEPASLVVVVCVENGGRGSA
                     AAGPVARAAINAAFPLEKKT"
     gene            complement(2289..2798)
                     /gene="mreD"
                     /locus_tag="P5079_07325"
     CDS             complement(2289..2798)
                     /gene="mreD"
                     /locus_tag="P5079_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRY29834.1"
                     /translation="MSPWLFGFSSFLVGWALQLVLLGIAFPASATPQWLMLSALALGG
                     MGKTDLAQTLGLLWGLSLDVFGVSLFGSQGFLLMLTGYVAGRASRQLNAEKPATQLTL
                     AFLGSFFFMAGLFVLEYFFRSLTAPRHFSFWTAFFEFALNLALAPLVFLFWRKWLELW
                     SRMEKERAS"
     gene            complement(2848..3669)
                     /gene="mreC"
                     /locus_tag="P5079_07330"
     CDS             complement(2848..3669)
                     /gene="mreC"
                     /locus_tag="P5079_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRY29835.1"
                     /translation="MLQRHLSEFLVALYTVFCLGCLSWSADSLVRTLKHTCLYLLSPS
                     LPVISQMETWGTFGRNMSRMIRLDDKYRRNETKWERDKLDELRLAALADENQRLSALL
                     GVSPFPDYSSRASRVLARDANDWFHSILIQKGLRHGVKLSDPVLTVQNERLALVGQVV
                     ELYERTCRVLLITDPLSAVSARLSRTGEEGAVEGQGMNRLVMHYLLSDSDVRVGDEVV
                     SAGLGHVFPEGVLLGTVVSVEGGSDESFKRVYLKPAVRISRLQEVLLLSRRSEGK"
     gene            complement(3774..4808)
                     /locus_tag="P5079_07335"
     CDS             complement(3774..4808)
                     /locus_tag="P5079_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY29836.1"
                     /translation="MFNYIFSLFSNDMGIDLGTANTLVYVKGQGIVLREPSVVAIERD
                     SKRALAFGLEAKKMLGKTPANIFAVRPLRNGVIADFEVTQEMIKYFIRRVHNRRSLLH
                     PRVVIGIPSGITEVEKRAVRESAEQAGAREVYLIEEPMAAAIGAEMPISEPSGNMIVD
                     IGGGTTEVAVISLGGMVVSKSLDVAGDEMDEAIVQYFRRKYNLLIGETTAEDVKIRIG
                     SVFPLPEEQTLDVKGRDQVTGLPKTITITSEEIRQALSEPLKLIIEVVKSTLEETPAE
                     LSADLVDRGIVLAGGGSLLRGFPELLSQETELPVTRAADPLTCVALGCGRYLEELDNM
                     KRYQRVLLSS"
     gene            complement(4899..5249)
                     /gene="rplQ"
                     /locus_tag="P5079_07340"
     CDS             complement(4899..5249)
                     /gene="rplQ"
                     /locus_tag="P5079_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY29837.1"
                     /translation="MKTHAGRKLSRPTGARMSLLRNMAASLFRHEQMRTTYPKAREVS
                     RFAEHVLAVAKTKTLTAYRQVGADIPDKEVRRKIFDVLVPRYQTRPGGCTRIHRLGRR
                     LSDGAEVAVIKLIQ"
     gene            complement(5305..6276)
                     /locus_tag="P5079_07345"
     CDS             complement(5305..6276)
                     /locus_tag="P5079_07345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRY29838.1"
                     /translation="MMTFGLVMPQKLETDTATLTPRYGKFTAGPFERGYGHTIGHSLR
                     RILLSSLEGAAVTAVRIKGAQHEFSSLKGVREDVINIILNLKQLRLKLYSSGPETLHL
                     KVKKEGEVKAKEIEANSNVEILTPDLVVANVDAGGDLDMEIEVSRGRGYISADRLRQE
                     GQPIGTIPTDALFSPVTKVHYEVENARVGQMTDYDKLIMEIWTDGSVTPADALAYAAK
                     IMKDSLSGFITFEEKEEPKAAPAPVADESTRTKELLSQPVDIIELSVRASNCLKAAKI
                     KTIGELVTKTEEELLNFKNFGKKSLDEIRDRLKELELHLGMKVGGGN"
     gene            complement(6363..7052)
                     /gene="rpsD"
                     /locus_tag="P5079_07350"
     CDS             complement(6363..7052)
                     /gene="rpsD"
                     /locus_tag="P5079_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY29839.1"
                     /translation="MARYIGPVCKVCRREGVKLFLKGERCSTKCVIDKTHGGAALPPG
                     QHAHRRAGKPTEYSKRLREKQKARRLSGVLERQFRRYFKVASGQKGNTGENLLRILET
                     RLDNVVRRLGFAASLAGARQQVLHGHVHVNGRCINIPSYQTKPGDRISLIDRLRGNFY
                     VKRSLEHAVQRGLPTWLELEGNLAEVVKKSSEAGALEGVALAGKVNMWPAREEMSYPV
                     NEQFIVELYSK"
     gene            complement(7159..7599)
                     /gene="rpsK"
                     /locus_tag="P5079_07355"
     CDS             complement(7159..7599)
                     /gene="rpsK"
                     /locus_tag="P5079_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRY29840.1"
                     /translation="MAEANEPKKTEKPAASKPAGAPAKVRKKIWKDIAVARVYIQSSF
                     NNTIVNITDERGNCIVWGTAGSAGFKGTKKGTPFAAQVTADVAAKKAVALGVKQVSVF
                     VKGPGSGRETAIRALQAAGLVILSIRDVTPIPHDGCRPPKPRRV"
     gene            complement(7639..>7802)
                     /gene="rpsM"
                     /locus_tag="P5079_07360"
     CDS             complement(7639..>7802)
                     /gene="rpsM"
                     /locus_tag="P5079_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY29841.1"
                     /translation="RHLIDIGTYRGLRHRRNLPVRGQRTKSNARTRRGKRKTVGASRA
                     QAPAAAATA"
BASE COUNT         1562 a         2466 c         2309 g         1465 t
ORIGIN      
        1 cgcttccact gggcccgtgc aggaacacgc tgcggccggc gccgatgtgc aggccggtga
       61 ggacgaacgg gacgatgagc ccgcgccatt cccgcagctc cctcaaatga aaatcggcat
      121 aggcggccag cgcgacggtg agggcaaggc gcgtcagctc caccggctga aaataaataa
      181 actgcaggtc gaaccaggac ttgctgccgc gcaatttgac tccgaaaacc aaaacgccga
      241 tcaagaacgc gaggctgatg aggtatatcc aacgataata ggtcaggagg acttggtagg
      301 gaaggaacac gatgagcgtg gcgccggcca gccccaccag cagggccgtg aattggcggc
      361 tgaggtaggc gtaggattga ccgccgtggg cggtggcgga aaagatgaac attagcccca
      421 tcaacgacag cgccaacacc gcgcccaaaa gtccccaatc caggcgggcg accagccgca
      481 ccacccacgg gtcgttggaa acccgataga acctcgggac gatctcggcg ctcacgtttt
      541 tttctccaag ggaaacgccg cgttgatcgc agcccgcgcc acgggaccgg cggcggcgga
      601 ccctcggccg ccgttttcga cgcacaccac caccacgagg gacgccggtt cccccggacg
      661 gcctgcgtag gcggcgaacc aggcgtgttc cctcccgtgc gggttctgcg ccgtccccga
      721 tttggcgccc acaaccaggt cgggtcgatg aacgccgcgg ccggtgccga ccgtgacgac
      781 gccctgcatg gcgagtccca gtctctccca gatgcgctgg ggcatttcga ccgtccgccg
      841 gagaacgggc gattgcttga agaggacgcg tccgcttcgg tccacaacgc tgtccatcac
      901 gaagggcttc cacagctggc ccccgttggc caccgacgac accatcatgg ccacctgcag
      961 cggcgtggcc gccacggccc cctgaccgat cgccaggttg gcggtgtctc cgtcgaacca
     1021 ggggactttc cgatattcct tcttccaggc gcggccgggg atgaacccgg ccgtttccga
     1081 cgtcaaatca atcccggttt tctcggcgaa gccgaaggcc cgcgccatcc gttcgatggc
     1141 gtccggcccg gtgcgaatcc ccaggttata gaaatagacg ttgcacgacc acgccacggc
     1201 gcccatgaaa tcctttacgt ggtgcactcc ccaacagccg aattccttgg accccacata
     1261 aaatttcccc ggacaattaa aagtctgctt gatgtcccag tcggcgtcga agagggccgc
     1321 ggcggcgtcg acgatcttga aaaccgagcc cggcgggtag gtcccctgaa tggcccggtt
     1381 gaacaacggc aggccgggat ccgtcagata ggggatgacg ctggcggacg ggtcgaaatc
     1441 cggagaactg gccagcgcca acaccgcccc ggaacgcggg tccaccacca cggcggcccc
     1501 cttcttcgtc tcggacgcct tcaacccctg ctcgacggct tcctgcaggc ggcggtcgat
     1561 ggtgagcgtg gcgagagggc cggggcgagg agcgatccgg cgcatgacgt gcacgtggcg
     1621 gccgccggcg tccatctcga actggatgcc gccgtcttcg ccccgcagga tattttcgta
     1681 gacgcgttcg atgcccatcc ggccgaccaa ctggcccatg cggtaccctt cttccctcag
     1741 gcgttccagc tcggccgagc tgatctcgtc cacgtatccc agcacgtggc tgcagaggga
     1801 gccgtaacgg gcgcggcgtt tcggttccac gaccacgctg acgccgggca acgaaagccg
     1861 tttttcaatc agagccaagg ccaccgggcg cgggatgtcc gccgaaatgc gggtcatttt
     1921 tcccgtcaaa cgggcctcgg cgaatttgcg cttaagcaac ggttccatgg cggggaacca
     1981 ccggaggacg tcgtcttcga cgttccgatg ggctttttca ggaagggctt gggcggaata
     2041 aaaaagagaa aaattgggtg cgttctccag caaaacgtcg ccgtggcggt ccatcaccac
     2101 cgcgcgcggc gcgcgaaggg gaatgatctg cgtccggttc atttcggcca aacgcgccat
     2161 ctccgaaccc cgcaggatct gcaactgaaa caggcgcaag cccagcaccg cgaaacaagc
     2221 cgacacgaag atcgtgatgg ctttcagtcg ccaatccgcc aacacatcca cgtgcggccg
     2281 attgtcggtc acgaggcccg ctccttttcc atccggctcc agagttcaag ccacttcctc
     2341 cagaaaagga agacgagcgg agccagggcg aggttcagtg cgaattcaaa gaaagcggtc
     2401 cagaacgaaa agtgccgcgg ggccgtcaac gaacggaaaa aatattccaa gacgaacagc
     2461 cccgccatga agaaaaaact gccgaggaac gccagggtca gctgggtcgc cggtttttcg
     2521 gcgttcagct gccgagaggc gcgccccgcg acatagcccg tcagcatcaa caggaaaccc
     2581 tgggagccga acaggctaac gccgaaaaca tcgagagaaa ggccccacaa aagccccagg
     2641 gtctgcgcca agtcggtctt tcccatgccc cccaaggcca aggccgaaag catgagccat
     2701 tgcggcgtgg ccgaggccgg gaacgcgatg ccgagcagga ccaactgcag cgcccagccg
     2761 acgaggaagc ttgaaaatcc gaacagccag ggactcatcg gcccccccca aaaaaaacgt
     2821 tttcctctgt cgaacgccgc cggcgcgtca tttgccctcc gaacggcgag acaagaggag
     2881 cacctcttgc aggcggctga tcctgaccgc cggcttcaga tagacccttt taaaactctc
     2941 atcgctcccc ccctccacag acaccaccgt cccgaggagg accccctccg ggaacacgtg
     3001 tcccaggccg gcgctcacca cctcgtcccc cacgcgcaca tcgctgtcgg acaagaggta
     3061 atgcatgacc aggcggttca tcccctgccc ctcgacggcc ccctcttccc cggtacggga
     3121 aagccgggcc gacacggccg acagaggatc ggtgatcaac aacacacggc aggtgcgctc
     3181 gtaaagctcg acgacctgcc ccaccagggc gagtctttca ttctggacgg tgagcacggg
     3241 atcggaaagt ttcaccccat ggcggagtcc cttctgtatc aaaatgctgt ggaaccaatc
     3301 attggcgtcc cgggccagaa cacggctcgc ccgggaagaa taatcgggaa acggcgacac
     3361 gccgagaagg gcggagagcc tttggttctc gtccgccaaa gccgccaaac gcagctcgtc
     3421 cagcttatcg cgttcccatt tggtttcgtt ccgacggtat ttgtcgtcca ggcggatcat
     3481 gcgggacata ttgcggccga aagtccccca cgtttccatt tggctgatca ccgggaggct
     3541 gggggagagg aggtacaggc aggtgtgctt gagcgtgcgg acaagggaat ccgccgacca
     3601 ggagagacaa cccaggcaga aaaccgtgta gagagctacc aggaattcgg acaaatgacg
     3661 ttgaagcaaa gaagcctccg cttcacaaaa tcccgcgagg gacccgggaa gctacacgag
     3721 gggagacgcc ggagaagaac cgaggggggg tgttcgacga gaaccggacc ctgtcaggaa
     3781 gaaagcagga cgcgctggta acgtttcatg ttatccagct cttccaagta ccgtccacaa
     3841 cccaaggcca cgcaggtgag ggggtcggcg gcgcgggtga ccggcaattc ggtttcctgg
     3901 gacaaaagct ccggaaatcc gcgcaaaaga ctgccgccgc cggccaaaac gatgccgcga
     3961 tccaccaaat cggcggacaa ttccgccgga gtttcctcca aggtggactt gaccacctcg
     4021 atgatgagct tgagcggttc ggacaacgcc tggcgtatct cttccgacgt aatggtgatg
     4081 gtcttgggca agccggtgac ctggtcccgc cccttcacat ccagagtctg ttcctccgga
     4141 agcgggaaga cgctgccgat acggattttc acatcctccg ccgtggtctc cccgatcaga
     4201 aggttgtact tccgtcgaaa atattgcacg atggcctcgt ccatttcgtc gcccgcaacg
     4261 tccagcgact tggagaccac catccctccg agagaaatga ccgccacctc cgtggtgccg
     4321 ccgccgatat ccacgatcat gttgcctgaa ggttcagaaa tcggcatttc ggcaccgatg
     4381 gcagcggcca tcggttcctc gatcaaatac acctcgcggg cgccggcctg ttcggccgac
     4441 tcccgcacgg cccgcttttc tacttccgtg atgccggatg gaatcccgat cacgacgcgc
     4501 gggtggagca ggctccggcg gttatggacc cgccggatga agtatttgat catctcctgt
     4561 gtaacttcga aatccgcaat cacgccgttc cgcagaggcc gaacggcgaa gatgttggcc
     4621 ggggttttcc ccaacatctt tttggcctcc aggccgaaag ccagcgcccg tttggagtcc
     4681 ctctcaatgg ccaccaccga cggttcacgg aggacaatgc cctgtccctt gacgtagacc
     4741 agggtattgg ccgtgccgag atcgatgccc atgtcgttgg agaagaggct gaagatgtag
     4801 ttaaacatgg gactcgtcga tggaaggacg ggagaccggt ttatccggaa cggcggggcc
     4861 actctttagc aagacatccc ccttaagagc gtgcaatcct actggatcag cttaatgacg
     4921 gccacttcgg ccccgtcgct caggcggcga ccgaggcggt ggatgcgggt gcatcctccc
     4981 gggcgcgtct gataccgagg aaccaggacg tcaaaaatct tccgccggac ctctttgtcc
     5041 gggatatccg cgccgacctg ccgataggcg gtcaacgttt ttgttttggc aacggccaaa
     5101 acgtgttcgg caaaacgcga cacctcccgc gctttaggat aggtggtgcg catctgttcg
     5161 tggcggaaaa ggctggccgc catgttccgc aaaagagaca tccgcgcgcc cgtgggccgg
     5221 gagagttttc gtcccgcatg cgttttcatg ttgttcgtcc gctcctttcc cgaagtccgt
     5281 cggcggcgac cgggcccgac ccggtcaatt tcccccgccg accttcatgc cgagatgaag
     5341 ttccaattct ttcagccggt cacggatttc atcgagggat ttcttcccga aattcttgaa
     5401 gttgagaagt tcctcttccg tcttcgtcac cagttcaccg atggtcttga tcttggcggc
     5461 cttcaggcaa ttggaggcgc ggaccgaaag ttcgatgatg tccaccggct gggacaaaag
     5521 ctccttggtg cgggtgcttt cgtcggcgac gggggccggg gcggccttgg gctcttcctt
     5581 ctcctcgaag gtgatgaaac cggagagaga atccttcatg atcttggccg cataggccag
     5641 ggcgtccgcc ggcgtcaccg atccgtccgt ccatatttcc atgatcagct tgtcgtagtc
     5701 ggtcatctgt cccacgcggg cgttttccac ttcgtaatgc accttggtga caggggagaa
     5761 gagggcgtcc gtcggaatgg tgccgatggg ctggccttcc tggcgcagcc ggtccgccga
     5821 gatgtagccc cggccgcggc tgacctcgat ctccatgtcc aggtcgccgc ccgcatccac
     5881 gttggcgacc accagatccg gcgtcaagat ttcgacgttg ctgttggctt cgatctcctt
     5941 ggccttaacc tccccttctt tcttcacctt cagatggagg gtctcgggac cggaagaata
     6001 gagtttcagg cgaagttgtt tgaggttgag gatgatgttg atgacgtcct ccctcactcc
     6061 cttgaggctg gagaattcat gctgggctcc cttgatgcgg accgcggtca ccgccgcgcc
     6121 ctccagggag gagagcagga tgcggcgcaa ggaatggccg atggtgtggc cgtaaccccg
     6181 ctcgaacggt cccgcggtga attttccgta gcgaggcgtc aatgtggccg tgtcggtctc
     6241 cagcttctgc ggcattacca atccaaatgt catcatgtgt caacttcctc cttgaatgtc
     6301 aacacccgtc cttcaaaacc gtcgttcttg cctgaaaagg accggcgaaa agatatcttc
     6361 tattatttgc tgtacaactc cacgatgaac tgctcgttca ccggatagga catctcctcc
     6421 cgggcgggcc acatgttcac tttccccgcc aaggcgaccc cctcgagcgc ccccgcctcc
     6481 gatgatttct tcacgacctc ggccaggttc ccctccagct cgagccaggt gggaagcccc
     6541 cgctgaacgg catgctccag ggaccgtttg acgtagaaat tgccacgcaa ccggtcgatc
     6601 agggagatgc ggtcccccgg tttcgtttga taggacggga tgttgatgca gcggccgttc
     6661 acgtgcacgt gcccgtgcaa cacctgctgg cgcgcccccg ccaaagaagc ggcaaaaccc
     6721 aggcggcgca ccacgttgtc caggcgggtc tccaaaatcc tgagaaggtt ctctccggtg
     6781 ttcccttttt gcccggaagc gaccttgaaa taacgccgga actgcctctc cagaactccg
     6841 gaaagacggc gagccttctg cttttcgcgc aggcgcttgc tgtactcggt gggcttcccc
     6901 gcccgacgat gggcgtgttg accgggcggc agagcggcgc cgccgtgcgt cttgtcgatg
     6961 acgcacttgg tggaacagcg ctcccccttc aaaaacagtt taacgccttc gcggcggcag
     7021 actttacaga caggtcctat gtatcgagcc acgcatgtct ccttatttta aaagttcaaa
     7081 gtgaaaagtt tgaagtcaga agtcgaatcg ttttcgaccc aaaacctcac acttccgact
     7141 tcttccccga aacttgtctt agacccttct cggtttgggc ggacggcacc cgtcgtgggg
     7201 gatgggcgtc acgtcccgga tggaaagaat caccaacccg gccgcctgaa gagcgcggat
     7261 ggccgtttcg cggccggagc cgggcccctt gacgaacacg gacacctgtt tcacgcccaa
     7321 cgccaccgct tttttggcgg cgacatcggc ggtcacctgg gcggcgaagg gggtcccttt
     7381 cttggtccct ttgaagcccg cgcttcccgc cgttccccaa acgatgcagt tcccccgctc
     7441 atcggtgatg ttgacgatgg tgttgttgaa cgaggattgg atatagacgc gggcgaccgc
     7501 gatgtccttc caaatctttt tccggacctt ggccggggcg ccggccggct tggaggccgc
     7561 cggcttttct gttttcttag gttcgttggc ttccgccata aatcaccgcc ttctggattt
     7621 ttcgaaaaag gactgccgtt aggccgtcgc ggccgcggcc ggggcctggg cgcgcgacgc
     7681 gcccaccgtt tttctctttc cgcgccgggt gcgggcattg gacttggtgc gctgtccgcg
     7741 caccggcagg ttgcgccgat gacgcaggcc ccgataggtg ccgatatcga tcaggtggcg
     7801 ga
//
LOCUS       DAOTFD010000116         7735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6677_length_7735_cov_4.430078, whole genome shotgun sequence.
ACCESSION   DAOTFD010000116 DAOTFD010000000
VERSION     DAOTFD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6677_length_7735_cov_4.430078"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5079_07365"
     CDS             <1..886
                     /locus_tag="P5079_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRY29842.1"
                     /translation="EFDDASNPEFLREGTAVRDFLKPDRIVIGVESKRAETALRQLYA
                     PLKAPLLVTDIKSAELIKHSSNSFLAMKISFINAVANVCERVGADVTRVAEGMGHDIR
                     IGRAFLNAGVGFGGSCFPKDLRAFINMAERVGYDFELLRAVQKINEGQQAWVVERLKK
                     CLWNLKHKTIAVWGLAFKPDTDDLRNAPALEIIERLQKEGCRIRAYDPVAADKAKTLL
                     KGVFFAKNPYEAAKGADAVVVVTDWQQIKRVDLKKVKSCVNTPVFLDGRNVFDPAAVS
                     NLGFQYHGVGRIPPAPSR"
     gene            complement(1023..2819)
                     /locus_tag="P5079_07370"
     CDS             complement(1023..2819)
                     /locus_tag="P5079_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HRY29843.1"
                     /translation="MNILGISCFYHDAAAALLKNGVLVAASEEERFSRKKHDSGFPIN
                     AIQFCLRKGALKMGDVDYVVFYEKPFRKFERLMMSILGTYPRSGAVFRESMTAWLGDK
                     LWLKSLIQERLGLPSEKILFMEHHLSHAASALFCSPFKEAAIMTVDGVGEWATATLGV
                     GHARWGEDEKNEITLFSELRFPQSVGLFYSAFTAFLGFEVNEGEYKVMGMAPYGEPRY
                     EDKVRKLLRLHEDGSFELDLSYFSYHYHTQKTISAKFIELFGEPRDPKARFVTVKTSL
                     FDDPVPPTRAELEKNQYYADIAASIQKVTEDIVIRMANHLHKRTGLKKLCLAGGVALN
                     SVANYRLLTETPFKEIYIQPAAGDAGGALGAALYVYHVCMGNRRRFILEHAYWGETFS
                     EETIASFLQENNIKNERIDDEESLMDRVVESLTAGKVVGWYQGAFEWGPRALGNRSIL
                     ADPRRAEMKDTVNIKIKFREPFRPFAPSVLSEKASNYFDLPHPEANYPARFMLVVSPV
                     KEDKRAAIPAVTHADGTGRLQTVVKETNPRYHRLIEKFGEATGVPVVLNTSFNLKGEP
                     IVTTPENAFNTFSKSGMDALVMGNRLLTKAEG"
     gene            complement(2824..3057)
                     /locus_tag="P5079_07375"
     CDS             complement(2824..3057)
                     /locus_tag="P5079_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29844.1"
                     /translation="MNDLLRSIWARWKKIAFAIGVFQTRLFFIMFYYVCLFPFAVALR
                     FFGDPLGLKLSPSSAWRKRTTRDTSLRHLRKQS"
     gene            3206..3730
                     /locus_tag="P5079_07380"
     CDS             3206..3730
                     /locus_tag="P5079_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRY29845.1"
                     /translation="MPDINEKTYQVLELISRDKELSQRDLATRSGLSLGMVNLLLKRL
                     IQTGYIKSAALNRKKMSYLLTPKGLSEKTSRSYQYLQRSIRTFQEARFRIENMIKKMI
                     SEGHRNFVILGEGEIADVVDVILSGYRSSGLIFRRSKEGNPPFETHEVVLDCRWGRKD
                     GAVGVSVLEEILKS"
     gene            3889..4881
                     /gene="gmd"
                     /locus_tag="P5079_07385"
     CDS             3889..4881
                     /gene="gmd"
                     /locus_tag="P5079_07385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRY29846.1"
                     /translation="MKRALITGITGQDGSYLAELLLEKGYEVHGMTRRSSVENFERIQ
                     HIQSKINLIQGDLSDQSSLDEAVKIIQPDEVYNLAAQSFVPTSWNQPVFTGFATGIGA
                     TRMLEAVRKHKPDARFYQASSSEMFGKVREVPQTENTPFYPRSPYGVAKVYAHYITVN
                     YRESYNMFACSGILFNHESPRRGLEFVTRKVTNTAAKIKLGLAKELCMGNLDAKRDWG
                     FAGDYVQAMWLMLQQPKADDYVVATGETHTVRELVDIAFSHVGLNPKDYVVVDPKFIR
                     PAEVDLLIGNPAKAKKALGWTPKVTFAELIRLMVDADIRLLKRQAAADAMGINR"
     gene            4903..5709
                     /locus_tag="P5079_07390"
     CDS             4903..5709
                     /locus_tag="P5079_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29847.1"
                     /translation="MIKSPLNLLRRLLQNRYMIQNMVVRDIRARYVGSFLGLFWTLIH
                     PLSQLVIYYFVFAVVLKVRLGPEFGGTHFAVWLIAGLLPWMLFAEVVGRSPNALMEQA
                     GLIKKTVFPSEILPFCHLSAALVNHFIALILLVTYISFFDAGLTARMSLLAPYLIFMG
                     LFAVGLSWFLSSLNVFLRDVGQVLGVALQIWFYLTPVIYPQNAIPSKYLFWLKLNPMY
                     HAVDGYRMALLGRVLPDTAGLLFLIASALSAFLIGGLLFKKLKPSFADVL"
     gene            5728..6975
                     /locus_tag="P5079_07395"
     CDS             5728..6975
                     /locus_tag="P5079_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29848.1"
                     /translation="MNAVELNGLSKIYRLYPSPKHRLWELLSMNRKVLHNDFYAVNDI
                     SLAVKKGETVGIVGQNGSGKSTLLEMIAGVLRPSKGAVHVNGRVSALLELGAGFNAEF
                     TGRENVYMNGALAGFNKKEMDVRLPEIESFAEIGEFIDQPVKTYSSGMFVRLAFSSAI
                     NMEPDILVIDEALAVGDVYFQHKCFSKLREFKEKGATLLFVSHDAVAVKGLCDRVVLL
                     ENGRLLKDGAPDETLDFYNGLITQKEQDKILQELDDSGRLRTRSGNFRAKIETVEIFG
                     ETGTLSKVFHVGENVEIRCRVRFHDEIVSPTIGIALRDRVGNEIFGTNSYHLSPYSGV
                     VKKDETLTARFRLPVNLGIGNYSLSVAVHSQDAHTVENYDWLDRATVLQIVPGTNSFF
                     VGSTYLPASLAWGKKDFNGKTKT"
     gene            6983..>7735
                     /locus_tag="P5079_07400"
     CDS             6983..>7735
                     /locus_tag="P5079_07400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY29849.1"
                     /translation="MTSQNSQSLDEIKRKIEESRIATNTLKYKFPVKLLNAPVGKSAA
                     GTVPNLSGNALTRAFNYIAEYGAGMAAGVKRFVKKIPVVNVVAQKTYHLLKREWWRGV
                     FGNIKNFPVVGECLWWAYMLLKTPQKLTQIFAHFQMLEKHVDDLEKQVNEQLMLIEKM
                     NRELADFCAEKFVTIDDDMYLALEREFRGPREMIRQRVSGYLPYLKGAGSTTQNPILD
                     IACGRGEWLEILKEHRLIAQGVDSNSSFVRQCR"
BASE COUNT         1799 a         2246 c         1970 g         1720 t
ORIGIN      
        1 ggaattcgac gacgcctcta atccggaatt cttgcgcgaa ggcacggccg tgcgtgattt
       61 cctcaaaccc gaccgcatcg tcatcggcgt ggaatccaaa cgggccgaaa cggccctccg
      121 tcaactctac gctcccctca aggcgccgct cctggtgacc gatatcaaaa gcgccgagtt
      181 gatcaaacac tcctccaact cgttcctggc catgaagatc tccttcatca acgcggtggc
      241 caacgtctgc gaacgcgtcg gagccgacgt cacccgcgtg gccgaaggga tgggccacga
      301 catccgcatc gggcgggcat ttctgaacgc cggggtgggg ttcggcggat cgtgttttcc
      361 gaaggacctg cgcgccttca tcaacatggc cgaacgtgtg gggtatgatt tcgaactgtt
      421 aagggccgtt cagaagatca atgagggaca gcaagcgtgg gtggtggaac gtctgaaaaa
      481 gtgcctatgg aacctgaaac acaagaccat cgccgtctgg ggattggctt ttaagccgga
      541 cacggacgat ctgcgcaacg cccccgcgct ggaaatcatc gaaagattgc agaaagaagg
      601 ctgtcgtatc cgcgcgtacg accccgtggc ggctgacaag gcgaaaacct tgctgaaggg
      661 ggttttcttc gccaaaaatc cctatgaggc ggccaaaggg gccgatgcgg tggtcgtggt
      721 cacggattgg caacagatca aacgggtgga tttgaagaag gtcaaatcgt gcgtcaatac
      781 gccggtgttt ctcgacggcc gaaacgtctt cgatcccgcc gccgtttcaa atctgggatt
      841 tcagtatcac ggcgtcggcc gcatcccgcc ggccccgtcc cgctaaaact ccaccgccgc
      901 ccccgcgctt gtcatccccg tcccaacctc aatcagaaga gggacggccc cgctgagttt
      961 agtggttggg gccgcatgcc tccatcgggg atccagaagc tttcccacga tttaccccgg
     1021 tcttacccct ctgccttcgt taacagccgg ttccccatca ccagcgcgtc catcccgctc
     1081 ttggaaaagg tattgaacgc gttttcaggt gtcgtcacaa tcggctcccc tttcaaattg
     1141 aaggaggtgt tcaagaccac gggcacccct gtggcctcgc cgaatttttc gatcagccgg
     1201 tgatagcggg ggttcgtctc cttgaccacg gtctgcagcc ggcccgtacc gtccgcgtgc
     1261 gtcaccgccg gtattgccgc ccgtttgtct tctttgacgg gagacaccac gagcatgaac
     1321 cgcgccggat aattcgcctc cgggtgggga agatcaaagt agttcgacgc cttttccgat
     1381 aacaccgagg gagcaaaggg acggaacggc tcgcggaact tgatcttgat gttgacggtg
     1441 tccttcatct ccgcccgccg gggatccgcc aaaatggatc gattacccaa ggcccgcggg
     1501 ccccactcga aagctccttg ataccacccc accactttcc ctgccgtcaa cgattccacc
     1561 acccggtcca tcaaactctc ctcgtcgtcg atgcgctcat tcttgatatt gttttcttgc
     1621 aggaaggagg cgatcgtctc ctcgctgaag gtttcacccc agtaggcgtg ctccagaatg
     1681 aaccggcggc gattccccat gcacacgtga tagacataga gcgcggcccc caaagccccg
     1741 cccgcgtccc cggcggccgg ctgaatatag atctccttga acggcgtttc cgtcaataac
     1801 cggtagttcg ccacggaatt cagcgccaca cccccggcca ggcacagctt cttcagaccc
     1861 gtccttttgt gcaaatggtt cgccatccga atcacgatat cttccgtcac tttctggatg
     1921 ctggccgcga tgtccgcata atattgattt ttctccaatt ccgcacgcgt cggaggcacc
     1981 ggatcgtcga acaaactggt tttgacggtg acgaaccggg ccttcgggtc gcgtggctct
     2041 ccaaagagct cgatgaattt ggcgctgatc gtcttttgcg tgtgatagtg gtagctgaaa
     2101 tagctcagat ccagctcgaa actgccgtcc tcgtgcaagc gcaacagttt tcggaccttg
     2161 tcttcgtaac gcggctcgcc gtagggcgcc atgcccatca ccttgtattc gccttcgttc
     2221 acctcgaatc ccagaaaagc cgtaaaggcg gagtagaaaa gccccaccga ctgcgggaag
     2281 cgcagctctg aaaaaagggt gatctcgttt ttctcgtctt ctccccagcg ggcatggccc
     2341 acgccgagcg tggccgtggc ccactcgccc acgccatcca ccgtcataat ggcggcttcc
     2401 ttgaaaggag aacaaaataa ggcgctggcc gcatgagaga gatgatgttc cataaaaagg
     2461 atcttttcag acggaaggcc gagacgttct tgtatcaagg atttcagcca aagcttgtct
     2521 cccagccagg ccgtcatgga ttcacggaac accgcccccg aacgaggata ggtgcctaaa
     2581 atggacatca tcaaacgttc gaatttccgg aaaggctttt cgtaaaaaac aacgtaatcc
     2641 acatcgccca tcttcagggc tccttttcgc aggcaaaact ggatggcgtt gatggggaaa
     2701 ccggaatcgt gcttttttcg ggaaaaccgt tcttcttcgg aggccgcgac caacaccccg
     2761 tttttcaaaa gggccgcggc ggcgtcgtga taaaaacagc tgattcctag aatattcatg
     2821 gcgtcaggat tgtttccgca ggtgccggag ggaagtgtcg cgcgtggttc gtttgcgcca
     2881 ggccgaggag ggggataact tcaagcccag cggatcacca aaaaatcgca gtgcgaccgc
     2941 aaaggggaac agacaaacat aataaaacat gataaaaaaa aggcgggtct gaaaaacgcc
     3001 gatggcaaaa gcgatttttt tccagcgtgc ccaaattgag cgcaataaat cgttcattcg
     3061 gtcttcacct tccctcgata tattaaatta gcaaatccgg atatatttca gcaaagcatt
     3121 tgactttagc gtgatttttt gatttaataa tacttcaccg cgtgacgcgg tttttttaat
     3181 aatctcagtg ttcaaaaatt gaacgatgcc ggatatcaac gaaaaaacat accaggttct
     3241 cgaactcatc tcccgggaca aagaactgtc tcagcgggat ttggccacgc gttccggcct
     3301 gtcgttgggg atggttaatc ttcttcttaa acgcctcatt cagactgggt acatcaaatc
     3361 ggcggccctg aatcgcaaga aaatgagtta cctgctcacg ccaaaaggct tgtccgaaaa
     3421 aacgtcccgc tcctatcagt atcttcaacg ctccatccgc acttttcagg aagctcgatt
     3481 ccgcatcgaa aatatgatca aaaaaatgat ttccgaaggc caccgtaatt tcgtcatcct
     3541 cggagagggt gaaatcgccg acgtcgtgga cgtcatcctt tccggttacc gaagttccgg
     3601 actcatcttt cgccgatcca aagaagggaa cccgccgttt gaaactcacg aggtcgtttt
     3661 ggattgccgc tggggccgga aagacggcgc cgtcggcgtg tcggttttgg aagaaatttt
     3721 aaaatcttag cccgattgaa aatgtgttcc gtcggacacg ccaaactatt ttgttcctgg
     3781 attccggcct aaaacatacc gcaatggcgg ccattttttc gtcgtccccg catggcttta
     3841 gcggggatcc agtggttttt aactgaaaag taaaaggaga accttaaaat gaagcgagcg
     3901 ttgatcacgg gcatcacggg gcaggacggc tcctatctgg cggaacttct cctggaaaag
     3961 ggttatgaag tccacggcat gacgcgccgc tccagcgtgg aaaattttga acggatccag
     4021 cacattcagt cgaagatcaa cctcatccag ggcgacctct cggaccaatc ctccttggac
     4081 gaagctgtca aaatcattca gccggacgaa gtctataacc tcgccgccca atccttcgtc
     4141 cccacctcct ggaaccagcc ggtcttcacc ggtttcgcca ccggcatcgg cgccacccgc
     4201 atgctggaag ccgtccgcaa acacaaaccc gacgcccggt tctatcaggc ttcctcgtcg
     4261 gaaatgttcg gcaaagtacg cgaagtcccg caaaccgaga acacgccgtt ttatccccgc
     4321 agcccctacg gcgtggccaa ggtttatgcc cactacatca cggtcaacta ccgcgaaagc
     4381 tacaacatgt tcgcctgctc gggaatcctc ttcaaccatg aatcgccccg ccgcggcctg
     4441 gagttcgtca cccgtaaagt tacaaacacc gcggccaaga tcaagctggg cctggccaag
     4501 gaactgtgca tgggcaacct cgacgccaaa cgggactggg gctttgccgg cgactatgtt
     4561 caagccatgt ggcttatgct ccaacagccc aaagcggacg attacgtcgt cgctaccggc
     4621 gaaacccata ccgtgcgcga actggtggac atcgcctttt cccatgtggg acttaatccc
     4681 aaagattacg tcgtcgtaga cccaaagttc atccgccccg ccgaggtaga cctcctcatc
     4741 ggcaatccgg ccaaagccaa aaaagccctg ggctggactc cgaaagtcac tttcgcggaa
     4801 ctgatccgcc tgatggtgga cgccgacatc cgcctcttga aacgccaagc ggccgccgac
     4861 gctatgggga tcaatcgtta agagaggaaa atgccggtct ctatgatcaa atcgcctctc
     4921 aacctcctgc gccggcttct gcagaaccgg tacatgatcc agaacatggt cgtgcgggac
     4981 atccgcgcac gttacgtcgg ttcgtttttg gggttgtttt ggaccctcat ccacccgctc
     5041 tcccaactgg tcatctatta tttcgtcttc gccgtcgtcc ttaaggtgcg cctgggaccc
     5101 gaattcggcg gcacccattt cgccgtctgg cttatcgccg gccttttgcc ctggatgctg
     5161 ttcgccgagg tggtcggccg ttcccccaac gccctcatgg aacaggccgg tctcatcaaa
     5221 aaaacagttt tcccttcgga aattctccct ttctgccacc tctcggccgc cttggtcaat
     5281 catttcatcg ccctgatcct actggtcacc tatatctcat tttttgatgc ggggctcacc
     5341 gcccgcatgt ccctccttgc gccctacctg atttttatgg gacttttcgc cgtgggcctg
     5401 tcgtggttcc tttcctcgct gaacgtgttc ctgcgcgacg tgggccaagt ccttggggtg
     5461 gcgctccaaa tctggtttta tttgaccccc gtcatttatc cccagaacgc catcccatcg
     5521 aaatatctct tctggctgaa attgaacccc atgtaccatg ccgtcgacgg gtaccgcatg
     5581 gcactcctgg gccgcgtttt acccgacacc gccggcttgc tgttccttat tgcctcagca
     5641 ctctcggctt ttctcatcgg cggattgctc ttcaaaaaac taaaaccctc cttcgcggac
     5701 gtcctgtaaa ggggaaagta agaccgaatg aacgccgtcg aactcaacgg gctttccaaa
     5761 atctatcgtc tctacccatc tcccaagcat cgcctatggg aactcttgtc catgaaccgc
     5821 aaggtcttgc acaacgattt ttacgcggtc aatgacatct cgctcgccgt caaaaaaggg
     5881 gaaaccgtcg gcatcgtcgg gcagaatggc agcggcaaaa gtactctgct ggaaatgatt
     5941 gccggcgtcc ttcgcccctc aaagggggcg gtgcacgtca acggccgggt ctcggccctc
     6001 ctcgaattgg gcgccggctt taacgcggaa ttcacaggcc gcgaaaacgt gtacatgaac
     6061 ggcgccctcg ccggattcaa caagaaagaa atggacgtcc gactgccgga gatcgagtcc
     6121 ttcgccgaaa tcggcgagtt catcgaccag ccggtaaaaa cctactccag cggcatgttc
     6181 gtgcgcctgg ccttctcctc ggccatcaac atggaaccgg acatcctcgt catagacgaa
     6241 gccctggccg tcggcgatgt ttatttccaa cataaatgtt tttcaaagtt acgggaattt
     6301 aaagaaaagg gggccacgtt gcttttcgtt tcccatgatg ccgtggccgt taaaggcctt
     6361 tgcgatcggg tggtgttgct ggaaaacggc cggcttctca aagacggcgc ccccgacgag
     6421 acgttggact tttacaacgg tctgatcaca caaaaagagc aggacaaaat cctccaagaa
     6481 ctggacgaca gcgggcgctt gaggacgcgc tccggcaatt tccgggccaa aatcgaaacc
     6541 gtcgaaatct tcggggaaac cgggaccctc tcgaaagtgt ttcacgtggg ggagaacgtt
     6601 gaaatccgat gccgcgttcg atttcacgat gagatcgttt ctcccaccat cgggattgcc
     6661 ctgagggacc gcgtcggaaa cgaaattttc ggcacgaaca gttaccacct ttctccctac
     6721 agcggcgtgg tgaagaaaga tgagacattg acggcccgct tccgcctccc cgtcaacctg
     6781 ggaatcggca actattccct cagcgtggcc gtgcattccc aagacgccca taccgtcgaa
     6841 aactacgact ggctggaccg cgcaacggtc ctgcaaatcg tccccgggac aaactcattt
     6901 tttgtgggtt cgacctatct cccagcctca ttggcatggg gaaaaaaaga ctttaacggg
     6961 aaaacgaaaa cctgaggaaa atatgacgtc ccaaaattcg caatctcttg atgaaattaa
     7021 acggaaaatt gaagagtcca ggatcgcgac gaatacgctc aaatacaaat ttccagtgaa
     7081 gcttttgaac gctcccgtcg gaaaaagcgc cgccgggacg gtccccaatc tctcgggcaa
     7141 tgcgctgact cgcgccttta attacatcgc cgaatacggg gcggggatgg cggccggcgt
     7201 caagcgtttc gtcaaaaaga ttcctgtcgt taatgtggtg gcgcaaaaaa cataccatct
     7261 tctgaaacgt gaatggtggc ggggagtgtt tgggaacatc aaaaactttc ctgtcgttgg
     7321 ggagtgcctt tggtgggcgt acatgctgct aaaaacaccc caaaaactaa cacaaatatt
     7381 cgcgcacttt caaatgctcg aaaaacacgt cgacgatcta gaaaaacaag tcaatgaaca
     7441 gttaatgctc attgaaaaaa tgaatcggga attggcagat ttttgcgctg aaaaattcgt
     7501 tacgattgac gatgacatgt atctggccct tgaacgcgaa ttcagaggtc cccgggaaat
     7561 gattcggcaa cgggtttctg gctacctgcc ctatttaaaa ggcgccggat cgacgactca
     7621 aaacccgatc ttggatatcg cttgtggtcg aggagaatgg ctggaaatat taaaggagca
     7681 tcggctgatt gcacaaggcg ttgactccaa ttcttctttc gttcggcagt gccgc
//
LOCUS       DAOTFD010000117         7704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6717_length_7704_cov_4.924304, whole genome shotgun sequence.
ACCESSION   DAOTFD010000117 DAOTFD010000000
VERSION     DAOTFD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6717_length_7704_cov_4.924304"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="P5079_07405"
     CDS             <1..445
                     /locus_tag="P5079_07405"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRY29850.1"
                     /translation="HGNLHTPVPGLREFPPPDRPPVNIVFQTYHAMVGIGMGLIGLSL
                     LGLFLWWRGALFVHRPWLFALFILSALGPQAANQLGWFSAEMGRQPWLVYGLLRTADG
                     LSKNVAAGQVLFSLFLFSAVYLLLGAAFLFLLLREIHHGPDERTA"
     gene            445..1461
                     /gene="cydB"
                     /locus_tag="P5079_07410"
     CDS             445..1461
                     /gene="cydB"
                     /locus_tag="P5079_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRY29851.1"
                     /translation="MDLNLVWYLLLGALFTGYVLLDGYDFGVGALHLFSEGEEERRTL
                     MNAVGPVWDGNEVWLVAGGGALFAAFPAVYAAVFSGFYPAVMLLLFALIARAVALEFR
                     GKLPAARWKKFWDAAFFAGSALAPFLLGVAAGNLALGLPLNAAGEFAAGVPFLLRPYA
                     LLIGALALSLSTLHGALYLLLKTDGPLRQKAHRWARKAFLLFLVLYVLATASTLLFVP
                     RFVAPLLEHPALWLVPLVTITALAVLFRALRRDRPLIALLASGTVIAGLMALFGLGLF
                     PHLVHSVPSPENSLTVYNAASSPKTLAIMLRIAALGLPPALAYTVVVHWIFRGKVKLD
                     PSGY"
     gene            1485..2987
                     /locus_tag="P5079_07415"
     CDS             1485..2987
                     /locus_tag="P5079_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29852.1"
                     /translation="MGPAPTSPDSRRTPPPFRRPEHRFSRLLTLAPILFLALFALDNL
                     RAPTLVHPEAYGRIVFYGTSAGRGLRGELEKIFDYRKGESYFFGEKIYRARLLNYAFD
                     LLDAKSLRFLNAFAPYGARSASHFLLTALAVLSLFFAFKSLRPGLPASSAALLAAFPA
                     TFPQLLVQNGFYFRPAKILSFFTAAFLFLVWTKLRTTDFKRLLQDKKKFILWLISVGL
                     LGLLTTADEQAIVMVAVFLLLMIAVSFRTRNFLPVLSFLLPALYFAVHTLFIDPWLAA
                     KFAGLKLDGGYPNPLRLFTLAPGLLTAALSVSLYQLQAVFGDPLDSGRWALAALALAL
                     FLLLGRRVFENRRRTKNAEPLGDILRPPLALLACLFAAFYAMTVRHPPIVAWPDLTRG
                     GYYYLPLALCFYLCFVHVLHRLGFLGKPANRVLLACVLILWAVGNFLAAASARQVIAT
                     GHLGAAVELQARMRKDLARPVDDLERAATSYPPGERLFLHWYRTHVGKTF"
     gene            2968..3843
                     /locus_tag="P5079_07420"
     CDS             2968..3843
                     /locus_tag="P5079_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29853.1"
                     /translation="MSEKLSERRLIFRLLYRPDSPVLLGLLLAAALAADTFLHAVGRP
                     DPRPAPHADITALWERTLRIAPQSALAHINLGRTFLDRGDPDRARRHFESAVAIDPHD
                     ATHWMLLGFARYGMNDPDGAVRAFQEALRVDPGMAMVHYNWGLLLERQGRTAEAMDHF
                     RRAETLDPSILEFSLLIAERSVRQKQFREAVARYENVRQKKPEQLGAHDYLHWGLALG
                     ALGADAEARPLLQRALALRPTLPDELNRTGVTLANQGNWTEAAACFEKALALRPDHPD
                     ALAHRRRALKKIPKP"
     gene            3907..4074
                     /locus_tag="P5079_07425"
     CDS             3907..4074
                     /locus_tag="P5079_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HRY29854.1"
                     /translation="MVLTRTATAVLLGALAGYLWYRLVGCASGACPITSNPYLSTLYG
                     ALMGLLIATSR"
     gene            4126..4722
                     /locus_tag="P5079_07430"
     CDS             4126..4722
                     /locus_tag="P5079_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY29855.1"
                     /translation="MLSEPALIEKSKQGDAEAFSSLIRRYEDKIYNLAHHVCASAPAE
                     ADDIYQETFLSAYKKIGQFRADSQLGTWLYRIAANLCWMRLRKKRREPFVPILDLPDA
                     GEHRHTGAPLDKMKSHADDPAKLAQKKELRQTVERALEELPVEYRLVAVLSDIEGLPN
                     DEIARILNLSLPAVKSRLHRARLFLRDRLSPLVKGHAS"
     gene            4719..4964
                     /locus_tag="P5079_07435"
     CDS             4719..4964
                     /locus_tag="P5079_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRY29856.1"
                     /translation="MKCRSLLRFLSDYVDGDLPTATARRIGDHNRVCPTCRRVIQTLK
                     TAVLCLKNTPRLHAAARARRALRRKIAALRRPGPPRT"
     gene            5335..5595
                     /locus_tag="P5079_07440"
     CDS             5335..5595
                     /locus_tag="P5079_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29857.1"
                     /translation="MSVKKFAFILIAGLSLGAPLRADQAVVESAVYVTRSGKSYHHDG
                     CRFLSWSKKRISISDAKKQYKPCSYCLPDLNLAQAPHHADRN"
     gene            5769..5975
                     /locus_tag="P5079_07445"
     CDS             5769..5975
                     /locus_tag="P5079_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRY29858.1"
                     /translation="MIKLNVGSWDRWLRLGVGVFIVSLAFWGPQTPWAWVGLVPVLTG
                     LFRFCPLYTVCGISTDKKSPSDGR"
     gene            5981..6622
                     /locus_tag="P5079_07450"
     CDS             5981..6622
                     /locus_tag="P5079_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY29859.1"
                     /translation="MPLLQERERDIIRKKLEELSGPVKLLLFKENVFCETCGVAEQLL
                     GELAALSDKLQTAVYNRLIDDQKAAEYGVERVPALVLEGPKGGRVRFFGIPGGYEFAT
                     LLEGLKDVAGGRADLAPHTRQKLSGLTTPVHIQVFVTSSCPFCPMAVRTAHKLAVEFP
                     NITADMVQAEEFPELSQKYNVSGVPKVVINDKVEFVGAQPETSFADAILRAVA"
     gene            7114..>7704
                     /locus_tag="P5079_07455"
     CDS             7114..>7704
                     /locus_tag="P5079_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29860.1"
                     /translation="MTPPPLDKKDYRFHRVADCAILLAGGAHFFYIFLFLFLKIPLLP
                     LVNVFSTLIWIACFFLNRTGLRKTGIFLGFGEILLHAAITVVLVGWPAGFHVYIICLA
                     PFIFFIPFPKRTPQLLLLLFIAVVYGGLFLYGIWNPPRYAVPDLVLWTLGCYNVAVLF
                     LSLGLMALYYEKAVAEAELAHAIAHAGGQPLTMKLSE"
BASE COUNT         1237 a         2943 c         2064 g         1460 t
ORIGIN      
        1 gcacggcaac cttcatactc cggtgccggg cctgcgcgag ttccccccgc cggaccggcc
       61 ccccgtgaac atcgtgttcc aaacctacca cgccatggtg ggcatcggca tgggcctcat
      121 cggactcagt ctgctgggcc tgttcctgtg gtggcgcggc gcgctcttcg tccaccgtcc
      181 ctggcttttc gccctgttca tcctctcggc cctgggcccg caggcggcca atcaactcgg
      241 ctggttctcc gccgaaatgg gccgccagcc gtggctggtg tacgggctcc tccgcaccgc
      301 cgacggcctc tccaaaaacg tcgcggcggg gcaggtcctt ttctccctct tccttttctc
      361 cgccgtctat ctcctgctgg gcgcggcctt cctcttcctc cttctgcgcg agatccacca
      421 cggcccggac gaaaggaccg cctgatggac ctcaacctcg tgtggtatct cctgctcggc
      481 gcgctgttca ccggatacgt cttgctggac ggctacgatt tcggcgtggg ggccctgcac
      541 ctcttttccg agggcgaaga agaacggcgc accctcatga acgccgtggg accggtgtgg
      601 gacggcaacg aggtgtggct ggtggccggc ggtggggcgc tgttcgcggc gtttccggcg
      661 gtgtatgccg cggtgttctc cggtttctac cccgccgtga tgctgctcct cttcgcgctc
      721 atcgcccggg ccgtggccct ggaattccgc ggcaaactgc ccgcggcgcg gtggaaaaaa
      781 ttctgggacg ccgccttttt cgctggaagc gccctggcgc ccttcctctt gggcgtggcc
      841 gcgggcaacc tggccctggg cctgccgctc aacgccgccg gcgaattcgc cgcgggggtc
      901 ccgttcctcc tgcgccccta cgccctgctc atcggagcgc tggccctgtc tctctccacg
      961 ctccacggcg ccctctacct cctcttaaag accgacgggc cgctccgaca aaaagcgcat
     1021 cgctgggcgc ggaaggcctt cctcctcttc ctcgtcctgt atgtcctcgc caccgcgtcc
     1081 acgctcctct tcgtgccgcg tttcgtggcg ccgctcctgg aacaccccgc gctgtggctc
     1141 gtcccgctcg tcaccatcac cgcgctcgcc gtgctcttcc gcgcgctccg ccgggaccgg
     1201 cccctcatcg ccctgctcgc ctccggcacg gtcatcgccg gcctcatggc cctcttcggc
     1261 ctcgggctgt tcccccacct ggtccactcg gtcccctccc ccgagaacag cctcaccgtg
     1321 tataacgccg cctcctcgcc caaaacgctg gccatcatgc tgcgcatcgc cgcgctgggc
     1381 ctgccgccgg ccctggccta caccgtcgtg gtgcactgga tcttccgcgg caaagtcaaa
     1441 ctcgacccct ccggctactg accgcgccca ctcccggcgt ccccatgggc cccgccccca
     1501 cctcccccga ttcccgccgc acgcccccac cgttccgccg gccggaacac cgcttctccc
     1561 gactgctcac gctcgccccg attctcttcc tggccctctt cgccctggac aacctccgcg
     1621 cccccacgct ggtccacccg gaagcttacg gacgcatcgt gttttacggc acctcggcgg
     1681 gccgcgggct ccgcggggaa ctggaaaaaa tcttcgatta ccggaaaggc gaaagttatt
     1741 tcttcgggga gaaaatatat cgcgccagac tgctcaacta cgccttcgac ctgctcgacg
     1801 ccaaatcgct gcggttcctc aacgccttcg ccccctacgg cgcccggagc gcctcgcatt
     1861 tcctcttgac ggccctcgcc gtcctctctc tcttcttcgc cttcaaatcc ctccggcccg
     1921 gtctccccgc ctcctccgcc gccctcctgg cggccttccc cgccaccttc ccccaactcc
     1981 tggtccaaaa cggcttctat ttccgaccgg ctaagatcct ctccttcttc accgcggcgt
     2041 tcctcttcct ggtctggacg aaactccgca caaccgattt caaacggctc cttcaggaca
     2101 aaaaaaaatt catcctctgg ctgatttccg tgggtctcct cggccttctc accaccgccg
     2161 acgagcaagc catcgtgatg gtcgccgtgt tcctcctgtt gatgatcgcc gtctcttttc
     2221 gaacgaggaa tttcctgccc gttctgagct ttcttcttcc cgccctctat ttcgccgtcc
     2281 acacgttgtt catcgacccg tggctcgcgg cgaaattcgc cggcctaaag ctcgacggcg
     2341 gctatccaaa cccgctccgg cttttcaccc ttgcgcccgg tctgctcacg gccgccctgt
     2401 ccgtctccct ctatcaactg caggctgtct tcggcgaccc gctggacagc ggccggtggg
     2461 ccctggcggc cctggcgctg gccctgttcc ttctgctcgg ccggcgcgtg ttcgagaacc
     2521 ggcgccgcac aaagaacgcc gagccgctcg gcgacatcct ccggccgcca ctggccctgc
     2581 tggcgtgtct tttcgccgcg ttctacgcca tgaccgtccg gcatccgccg atcgtcgcct
     2641 ggcccgacct cacccgcggc ggctactact acctgcccct ggcgctgtgt ttctaccttt
     2701 gtttcgtcca cgtcctccac cgtctcggat tcctgggaaa acccgcgaac cgcgtgctct
     2761 tggcgtgcgt gctgatcctg tgggccgtcg gcaatttcct cgccgccgcc tccgcccgcc
     2821 aggtcatcgc gacgggacac ctcggcgccg cggtcgaact ccaggcgcgg atgcgcaaag
     2881 acctcgcccg ccccgtcgac gatctcgaac gggccgccac ctcctatccg ccgggcgaaa
     2941 gactgttcct gcactggtat cgaacccatg tcggaaaaac tttctgaacg ccgcctgatc
     3001 ttccggttgc tgtatcgccc cgactcgccg gtcctgctgg gcctcctgct cgcggcggcg
     3061 ctcgcggccg acacgtttct ccacgccgtg ggccggcccg acccgcggcc cgcgccgcac
     3121 gccgacatca cggccctctg ggaacgcacc ctccgcatcg ccccgcaatc cgccctcgcg
     3181 cacatcaatc tgggccgcac ctttctggac cgcggagacc ccgaccgcgc ccggcgccat
     3241 ttcgaaagcg ccgtcgccat cgacccccac gacgccaccc attggatgct gctcggcttt
     3301 gcccggtacg ggatgaacga ccccgacggc gcggtccggg cgttccaaga agccctgcgg
     3361 gtcgaccccg gcatggccat ggtccattac aactgggggc tccttctcga acggcaaggc
     3421 cgcacggcgg aagcgatgga ccatttccgc cgcgccgaga cgctcgaccc cagcatcctg
     3481 gaattctccc tgctgatcgc cgagcggtcg gtccggcaga aacagtttcg ggaagccgtc
     3541 gcccgctacg aaaatgtccg tcagaaaaaa ccggaacagc tcggcgccca cgattacctc
     3601 cactggggac tggcgctcgg cgccttgggc gcggacgccg aagcccggcc cctcctccaa
     3661 cgcgctctgg ccctgcgccc gaccctgccc gacgaactca accgcaccgg cgtgacgctg
     3721 gcgaaccagg ggaactggac cgaggcggcg gcgtgtttcg aaaaagccct ggccctccgg
     3781 cccgaccacc cggacgccct cgcccaccgg cggcgggccc tcaaaaaaat cccgaaaccc
     3841 tgaccccctt cccgaatcct tttcacgcct ccgcgcatcc aaagaaggaa acggaggtga
     3901 aaacccatgg tcctcacacg aacggcgacc gccgtgctcc tgggcgcgct ggccgggtat
     3961 ctgtggtacc ggctcgtcgg ctgcgcctcc ggcgcctgcc ccatcacgtc caacccgtac
     4021 ctcagcacgc tctacggcgc cctgatggga ctcctcatcg ccacctcccg ctgagcggct
     4081 ttttcttccg ggcccaccgg acttttctgc tagaataaat ccgtcatgct gtccgaaccg
     4141 gcgctcatcg aaaaaagcaa acagggcgac gccgaggcct tctcctccct catccgccgc
     4201 tacgaggaca agatctacaa cctcgcgcac cacgtctgcg cctccgcccc ggccgaggcg
     4261 gacgacatct accaggaaac cttcctctcc gcgtacaaga agatcggcca gttccgcgcc
     4321 gattcccagc tgggcacctg gctctaccgc atcgccgcca acctctgctg gatgcgcctc
     4381 cggaaaaaac ggcgcgaacc gttcgtcccc atcctggacc tgccggacgc cggggaacac
     4441 cgccacaccg gggcgccgct ggacaaaatg aagtcgcacg ccgacgaccc cgccaaactg
     4501 gcccagaaaa aagaactccg ccaaaccgtg gaacgcgccc tggaagagct ccccgtcgaa
     4561 taccggctgg tggcggtgct cagcgacatc gaaggcctcc ccaacgacga gatcgcccgc
     4621 atcctgaacc tcagcctgcc ggcggtcaaa tcccggctgc accgggcgcg gctgttcctg
     4681 cgggaccgcc tcagcccgct cgtgaaggga cacgcctcat gaaatgccgc tcgctcctcc
     4741 gcttcctttc ggactacgtg gacggcgacc tgccgacggc gacggcccgc cgcatcggcg
     4801 accacaaccg cgtgtgcccc acctgccgcc gcgtcatcca aacgctcaag accgcggtgc
     4861 tctgcctgaa gaacaccccc cgcctccacg ccgccgcccg cgcgcggcgg gccctgcgcc
     4921 gaaagatcgc cgccctccga agacccggcc ctcctcgcac gtaaaaaccc gccgcctccc
     4981 acggcgcgcc cggccccgcg ccgcgcaagc tttttggccg gccccctccc gcaccgaaaa
     5041 aattttttaa aaaatccttg aaattttccg gggaggcgtg tatactgatg cacgttttac
     5101 ttcccggggc agcgagttac ccaagacttc ctgcgcgccg aaacccggaa agcggaacga
     5161 tgagctggtt tttcggtttt cgtgtttggc gcagcaacag ggcagcgatc gtttagttca
     5221 ttccgactaa catccgctaa aactacacca acgccaacga accgccactc gcacacgcgc
     5281 aacccttttt gttgcgctac gccgggtttt atctatctat ccttggaggt cgtcatgtct
     5341 gtcaagaagt tcgctttcat cctcatcgcg ggactctccc tcggggcgcc gctccgcgcg
     5401 gaccaggccg tcgtcgagtc ggcggtctac gtcacccgca gcggcaaaag ctaccaccac
     5461 gacggatgcc gattcctgtc ctggagcaaa aaacgcatct ccatttccga tgcgaaaaaa
     5521 caatacaaac cttgctccta ctgcctgccg gacctcaatc tggcgcaggc gccgcatcac
     5581 gccgaccgga actaattaac accccccccc cccccccccc cccgcccgag gcgaccccca
     5641 cccgcctccg ggccgaagcc gtttcccccc aaaaagccgc gaatcctttc ggtccaccca
     5701 ggcatccaaa gagtgaaggg accggacccc ctcgtcggcc ctcgtccaag gaaaaaagga
     5761 gaatcgtcat gatcaaactc aatgtcggct cgtgggaccg ctggctccgc ctcggggtcg
     5821 gggtcttcat cgtgtctttg gcgttctggg gaccccaaac gccgtgggcg tgggtcgggc
     5881 tggtgcccgt tctcacgggg ctgttccgtt tctgtccgct ttacacggtc tgcggcatct
     5941 ccacagacaa aaaatccccc tcggacggga ggtgagcgcc atgcctcttc tgcaagaacg
     6001 tgaacgcgac atcatccgga aaaaactgga ggagctctcc gggccggtga aactcctcct
     6061 cttcaaggaa aacgtcttct gcgaaacctg cggggtggcg gaacaactgc tgggcgaact
     6121 ggcggcactg tccgacaaac tgcagaccgc ggtctacaac cggctcatcg acgaccaaaa
     6181 agcggcggaa tacggcgtgg aacgcgtccc ggccctggtc ctggaaggac ccaaaggcgg
     6241 ccgcgtgcgg ttcttcggca tcccgggcgg ctacgagttc gccaccctgc tcgaagggct
     6301 caaagacgtg gccggcggcc gggccgacct cgccccccac acgcgccaga aactcagcgg
     6361 gctcaccacc ccggtgcaca tccaggtgtt cgtcacctcc agctgtccgt tctgtcccat
     6421 ggcggtgcgc accgcgcaca agctggcggt ggagtttccg aacatcaccg ccgacatggt
     6481 ccaggcggag gagttcccgg aactgtccca aaaatacaac gtcagcgggg tgcccaaggt
     6541 ggtcatcaac gacaaggtgg aattcgtcgg cgcgcagccc gagacctcct tcgccgacgc
     6601 catcctccgc gcggtagcct gaggggttgt cagcgctctg cgctgccgat ggccccaaag
     6661 ctatgcgtgg gacgtgacga gtcttcactc ctctggagtg ccgatcgtcc caaagccctg
     6721 cgtgggacgt gaggggtctt cactcctctg gagtgccgat cgtcccaaag ccctgcgtgg
     6781 gacgtgacgg gtcttcactc ctctcccgac ctacataccg ggacgttatc tagcgacaac
     6841 gtggagcgcg tcaggtcctc aaagtgatac gtccgtgtcg ctagacacgg gccctgtttg
     6901 tggaaagggc gggaccgcca cctccccatt tccctttgcc gccagacatg gggctgtttt
     6961 gtcgcacagc cttgggcgtt ccccgaagtc cgctccgtgc cccccctgtt tttgctccgc
     7021 cctgtctgtg ccgtctcact tatttgttaa gattgtccct ggcatgccac taaaaaacag
     7081 accgtcgact tttcccgggc gttcgaacca accatgaccc ctccacctct cgacaaaaaa
     7141 gattaccgtt tccaccgcgt cgccgactgc gccattctcc tggccggggg cgcccatttc
     7201 ttctacatct tcctgttcct cttcctgaaa atccccctct tgcccctcgt caacgttttc
     7261 agcacgctca tctggatcgc ctgtttcttc ctcaaccgca ccggactccg caaaaccggc
     7321 atcttcctcg gcttcggcga gatcctgctc cacgcggcca tcaccgtggt gctggtgggg
     7381 tggccggccg ggttccacgt ctacatcatc tgcctggcgc cgttcatctt cttcatcccg
     7441 ttccccaaac ggaccccgca actcctcctg ctgttgttca tcgccgtcgt ctacggcggc
     7501 ctgttccttt acggcatctg gaatcctccc cgctacgccg tccccgacct ggtcctctgg
     7561 acgctcggat gctacaacgt ggcggtgctc ttcctctccc tggggctcat ggcgctctac
     7621 tacgaaaaag ccgtcgcgga ggcggaactg gcccacgcca tcgcccatgc cggcggccag
     7681 ccgttgacaa tgaaactctc cgaa
//
LOCUS       DAOTFD010000118         7636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6801_length_7636_cov_4.074528, whole genome shotgun sequence.
ACCESSION   DAOTFD010000118 DAOTFD010000000
VERSION     DAOTFD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6801_length_7636_cov_4.074528"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="P5079_07460"
     CDS             complement(<1..2254)
                     /locus_tag="P5079_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY29861.1"
                     /translation="MGPTTFTDKKIRYFAAMFLVSAIALAAAGGVYYSFLKKNLFNKK
                     KNDLSVIADLKVNQLASWHRERLNDAEMLSRDVLLREVLWSYRTSPTPQKETRLREWM
                     AALQETYRYMNVFLLDPRGQLLLAGPGTSRYSERYHAPFARDAAAVGRVAVMDIHQDE
                     GDEEGDRAIHIGLFIPIWRSGRVLGLVAIQVDPAIVVYPLLQSWPTVSPTGEILLVRP
                     EGEEVVVLNELRRRKDAALALKFPLTDTGLPFVAAVRGQEGIFQGLDYNGRRVLAALR
                     KVPSTPWFLVAKWDEEEVYADLFRQRYLLAVGVVLGIAFAGGGLMALWRRRQIRYYRL
                     ALELERERLVLVKHYDYLAKYTNDIMLLVERDSRRIMEVNDRAVAAYGYSREELLGLR
                     LQDILAPDMRPLMPKRVENIFRENGSRFESRHRRKNGETFDVEVSARPIEIEGKGYFQ
                     AILRDIGERKAAEAALRESEEKYRELTEQIHDIVYSVDPAGTITYISPQVARYGYTPG
                     DLMSHGFLEFIVPEDRETVLRDFKETMATGVEHPTMVRILGRDGQAHWIEERGRLRRD
                     ASENVTGIVGVIRDITESRRAEAALRESEERYRTLASIASEGIMIHRGGEICEANQVF
                     AELAGFSSPQELIGKEGLRMVRFTPESEKKIRDHMRTGTTETYDLELVHPSGRLVPVE
                     TRGRDITFQGRPARLIYMRDITERKQAETALRESQRRFEQTTEESREMVWEVDAAGRT
                     IAFEPAFPVEP"
     gene            2352..3572
                     /gene="glgA"
                     /locus_tag="P5079_07465"
     CDS             2352..3572
                     /gene="glgA"
                     /locus_tag="P5079_07465"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRY29862.1"
                     /translation="MKALFLTNEYPPHIYGGAGIHVEYLTRELAKRIGVEVRCFGEQD
                     FSKGSLRAKGYAPPTAAFKKCDPRLKSPLAAFANGIAFNADPTKAHLVHCHTWYSHFG
                     GLLAKMAYGLPLVITTHSLEPLRPWKREQLGRGYDLSTWVEDTALRAADAVIAVSRGM
                     KTDILRLFDVDEKKIRVIHNGIDVEEYRARKNPATLRKYGIDPAAPYVLFFGRITRQK
                     GIIHLVNAVKYLSPKTQVVLCAGQPDTPEIQREMEQSVRKVQKKRTNLRWIREWLDTR
                     AKIEIYSHAAAFCCPSIYEPFGIINLEAMACGVPVVGSAVGGIKEIVEHGKTGYLVPL
                     AQQKQSPFEALSPDRFARDLAAPLNRLLADDALRDRLGRASRRRVEQFFSWKSIARQT
                     LELYKTLIRRGKNG"
     gene            3565..5613
                     /locus_tag="P5079_07470"
     CDS             3565..5613
                     /locus_tag="P5079_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HRY29863.1"
                     /translation="MADKKSKKKNPAAHQAARPAALPPSPFVIENVRPAVDGGRYPVK
                     REAGETLAVTATVFKEGHDAVVVLLKYREKYGDKTWRETEMTCVNPGLDVWQGKFLLE
                     KNTRYEYTVEAYTDFYRSWLKDTLKKFDAGQDVASELLEGRHFLETEQKRLPKSDYLE
                     RVLAGLKSARNQGDQLKIFSEDRLLEFTKTCSERTDASVLEPPLEVIADRVQARFAAW
                     YEMFPRSQGKTAGKSGTFKDAEARLKDIQAMGFDVLYLPPIHPIGVSHRKGPNNSLKA
                     GPDDPGSPWAIGNAHGGHFAVEPGLGTLADFEKFEKACRAHGIEIALDFAINCSPDHP
                     YVKEHEEWFFKRPDGSIKYAENPPKKYEDIYPLNFYAADGHWRPLWEEMKKILDFWIG
                     KGVRIFRVDNPHTKPVFFWKWLIEDIQREHPDVLFLAEAFTRPPMMKMLAKAGFSQSY
                     TYFTWRQFKQELTDYHTELTQSEMKDYFRGNLFANTPDILPLILQEGGRPAFKSRFVL
                     AATLSSVYGIYSGFELCENKALPGKEEYADSEKYEFKARDWDKPGNIKDLIAKVNRIR
                     RENPALQHYKNLRFYRTDNDNVLFYGKATDDKKNVVLVAVNLDPYRAQETTLHIPIAE
                     FGIKPDETYQLQNLLAGDRLLCKGDKIIVRLDPKDEPAHIYQLNRWMKREQDFDYFGM
                     "
     gene            complement(5720..>6061)
                     /locus_tag="P5079_07475"
     CDS             complement(5720..>6061)
                     /locus_tag="P5079_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29864.1"
                     /translation="DKKGVNMNVYYVASAELFGLLSDKEQEKIMPTERTYEAMGITDF
                     TLPTMYQWVRSKEGLKRTLHSFCGNHYLGSGQAHKVLEEAGVHAEGQLKAILEYARMA
                     EKTYRPGKKGK"
     assembly_gap    6062..6071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6072..>7636)
                     /locus_tag="P5079_07480"
     CDS             complement(<6072..>7636)
                     /locus_tag="P5079_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29865.1"
                     /translation="KSKALDGHPTPLTPFIRIATGASGVGVGASIGLAFGAMDTYGAK
                     APWVHMVEGEGGMTPGRVHEAMAACATSQLYNVVMHVDWNQASIDSNHVCAENGQPGD
                     YVQWTPMELAYLHDWNVIYVPDGKDFRQVFAAQKLANSLTNKMPTAVVYRTVKGWRYG
                     IEGRSSHGAGHKFCSDGYYATLKEFEDAFSVSFPRFSGNPAVEAETEKCFYDTLLVFR
                     AVLEKEKETTAYLGRCIADSKARLDKQSRTVRADAPRIGEIYKSDVKPETPPAGLTPD
                     PGKSITLRDVMGDTLNALNKISGGGIFAASADLMGSTSVSNINKGFPQGFYNAEKNPG
                     SRALSLGGICEDGMGAVMAGLASFGNHIGAISSYAAFIAALEHIAARLHSIGQQSRKQ
                     LTGEPFKPFIMINAHAGVKTGEDGPTHADPQPLQLLQENFPKGFVITLTPWDPQEIWP
                     LMIAALKARPAVICPFVTRPAESVPDRKALGLPPAHVAAKGVYAIRTADPKAKQYNGT
                     VVLQGNGVASVFV"
BASE COUNT         1509 a         2556 c         2157 g         1404 t
ORIGIN      
        1 cgggctcgac tggaaaagcg ggctcaaagg cgatcgtccg gccggcggca tccacttccc
       61 acaccatttc ccggctttct tcggtggtct gctcgaaacg tctctggctc tcccgcaggg
      121 ccgtctcggc ctgttttctt tccgtgatat cgcgcatgta gatgagtctc gcgggacgac
      181 cctggaaagt gatgtcccgt ccccgggttt cgacggggac gaggcgtccg ctcggatgga
      241 ccagttccag gtcgtatgtt tccgtggtgc cggtgcgcat atggtcccgg atcttctttt
      301 cggactccgg cgtgaaacgg accatcctta atccttcttt gccgatgagc tcctgcgggc
      361 tcgaaaaacc cgccaattcg gcaaacacct gattggcctc gcagatctcc cctccccggt
      421 gaatcatgat cccctcggaa gcgatggagg ccagagtccg atagcgttcc tcgctctccc
      481 gcagggccgc ctccgcccgc cggctttcgg tgatgtcccg gatgacgccg acgatgcccg
      541 tcacgttctc cgaagcgtcc cgccggagcc ggccccgctc ctcgatccaa tgggcctggc
      601 cgtcgcggcc gaggatgcgc accatcgtgg gatgttcgac ccccgtggcc atggtctcct
      661 tgaaatcacg gaggaccgtc tcccgatctt cgggcacgat gaattccagg aaaccgtgcg
      721 acatcaggtc gccgggcgtg tacccgtaac gcgccacctg cggactgatg taggtaatgg
      781 tccccgcggg gtccacggag tagacgatgt cgtgtatctg ctccgtcagc tcgcgatatt
      841 tttcctcgct ctcccgcagg gcggcctccg cggcctttct ctcgccgatg tcccgcagga
      901 tggcctgaaa ataccctttc ccctcgatct caatgggccg cgcgctgacc tcgacgtcga
      961 aagtttcccc gttcttgcgg cgatgccggc tttcaaaacg cgaaccgttt tcccgaaaaa
     1021 tgttctccac ccgtttcggc atgagaggcc gcatgtcggg cgccaatatg tcctggagac
     1081 gcagacccaa caactcctcc cgggaatatc cgtaggcggc caccgcccgg tcgttcactt
     1141 ccatgatcct ccggctgtcc cgttccacga ggagcatgat gtcgttggtg tatttggcga
     1201 ggtaatcgta gtgtttcacc agcacgaggc gctctcgctc caattccagc gcaaggcggt
     1261 aatatcgtat ctgccggcga cgccacaggg ccatcagccc gccgccggca aacgctattc
     1321 ccaaaaccac gcccacggcc aggagatacc tctgccggaa caaatcggca tagacctcct
     1381 cttcgtccca cttggccacc aagaaccagg gggtcgacgg cactttccgc aacgccgcca
     1441 agaccctcct gccgttgtag tccagcccct gaaaaatccc ttcctggccc cgcaccgccg
     1501 ccacaaaggg caacccggtg tccgtcaggg gaaatttcaa cgccagagcg gcgtctttcc
     1561 gacggcgcaa ttcattgagc accaccacct cttccccttc cggccgcacc aggaggattt
     1621 ctccggtcgg gctgaccgtg ggccaggatt gaagcagggg atagaccaca atggccggat
     1681 ccacctggat ggcgacaaga cccagaacgc ggccgctcct ccaaatcggg atgaaaagtc
     1741 cgatatggat ggcgcgatcc ccctcttcgt ctccctcatc ctggtggatg tccatgaccg
     1801 ccacccggcc gaccgccgcc gcgtcgcgcg caaagggcgc gtgataccgt tccgaataac
     1861 ggctggttcc cgggccggcc aacaggagtt gcccccgggg gtccaggagg aaaacgttca
     1921 tataccggta cgtctcctgg agagcggcca tccactcccg cagacgcgtt tccttctgcg
     1981 gagtagggga ggtccgatac gaccacaaga cctcccgcag caggacgtcg cgggacagca
     2041 tttcggcatc gttcaggcgt tcccggtgcc acgacgccaa ctggttcact ttcaggtcgg
     2101 cgatgacgga gaggtcgttc tttttcttat tgaagaggtt cttcttcagg aaggaataat
     2161 aaacgccgcc ggcggcggcg agggcgatgg ccgaaaccag aaacatcgcg gcgaaatatc
     2221 ggattttttt atcggtgaaa gtcgtcggac ccatgggctc cctcaggcgt ccattatacg
     2281 tcttatcttt ttatggtggc ccgtgtttta ttttttttcc gcaaagagaa tgttataatt
     2341 ttacccacac catgaaggca cttttcctaa ccaacgaata tcccccgcac atctacggcg
     2401 gcgccggcat ccatgtggaa tatctgaccc gggaactggc caaacgcatc ggggtggagg
     2461 tccgctgttt cggcgagcag gatttttcaa aaggctccct gcgggccaag ggatacgccc
     2521 cgcccaccgc cgccttcaaa aagtgcgacc cccggctcaa gtccccgttg gccgccttcg
     2581 ccaacggcat cgccttcaac gccgacccga cgaaggccca cctcgtccac tgccatacct
     2641 ggtacagcca ttttggcgga ctcctggcga aaatggccta cggcctcccg ctggtgatca
     2701 ccacccactc cctggaaccg ctccggccgt ggaaacgcga gcagctgggc cggggctacg
     2761 acctctccac ctgggtggag gacaccgcgc tccgcgcggc cgacgccgtc atcgccgtct
     2821 cccgcggcat gaaaaccgac atcctccggc tcttcgacgt ggacgaaaaa aagatccggg
     2881 tcattcacaa cggcatcgac gtggaggaat accgcgcccg gaaaaatccc gccacgctcc
     2941 ggaaatacgg catcgacccg gccgccccct acgtcctctt cttcggccgc atcacccgcc
     3001 aaaaaggcat catccacctg gtcaacgccg tcaaatacct ttctcccaaa acacaggtcg
     3061 tcctctgcgc cggccagccg gacaccccgg agatacaacg ggagatggaa cagagcgtcc
     3121 gaaaggtcca gaaaaaacgg acgaacctcc gctggatccg cgaatggctg gacacccgcg
     3181 ccaagatcga gatctattcc cacgccgccg cgttctgctg cccgtccatt tacgagccgt
     3241 tcggcatcat caacctggag gccatggcct gcggcgtgcc ggtggtgggc agcgcggtgg
     3301 gcggcattaa ggagatcgtg gaacacggaa aaaccggcta cctggtgccg ctggcccagc
     3361 agaaacagag ccccttcgag gcgctctcgc ccgaccggtt cgcccgcgat ttggccgcac
     3421 cgctgaatcg cctcctggcg gacgacgcgc tccgcgaccg gctgggccgc gccagccgcc
     3481 gacgcgtgga acaatttttc agctggaaga gcatcgcccg gcagaccctg gaactctata
     3541 aaacccttat ccgacgagga aaaaatggct gacaaaaaat ccaagaaaaa aaatccggcg
     3601 gcccaccaag ccgcccgccc cgcggccctt ccgccgtcgc cgttcgtcat tgaaaacgtc
     3661 cggcccgcgg tcgacggcgg ccggtatccc gtcaagcgcg aggccggaga gaccctcgcc
     3721 gtcaccgcca cggtgttcaa ggaaggacac gacgccgtcg tcgtcctgct caaatatcgg
     3781 gaaaaatacg gggacaaaac ctggcgggaa acggaaatga cctgcgtcaa cccggggctg
     3841 gatgtctggc agggcaaatt cctcctcgaa aagaacaccc gttatgaata caccgtcgag
     3901 gcctacaccg atttttatcg ttcctggctc aaagacacct tgaagaaatt cgacgccggc
     3961 caggacgtgg ccagcgaact gttggaaggc cggcatttcc tggaaaccga acaaaaacgt
     4021 cttccgaaga gcgactacct ggaacgcgtg ctcgccggcc tgaaatcggc caggaaccag
     4081 ggcgaccagc tgaagatctt ctccgaagac cgcctcctgg aattcacgaa aacctgttcc
     4141 gaacgcacgg acgcctcggt gctggagccc cccctggaag tcatcgccga ccgcgtccag
     4201 gcccgtttcg ccgcctggta tgagatgttt ccccgctccc agggaaaaac cgccggcaag
     4261 agcggcacct tcaaggacgc cgaggctcga ctgaaggaca tccaggccat ggggttcgac
     4321 gtcctctacc tgccgcccat ccaccccatc ggcgtctccc accgcaaggg acccaacaac
     4381 agcctcaagg cgggtcccga cgaccccggc tcgccctggg ccattggcaa cgcgcacggc
     4441 gggcatttcg ccgtggagcc cggcctcggc acgctggcgg atttcgagaa attcgaaaaa
     4501 gcctgccgcg cccacggcat cgagatcgcc ctcgacttcg ccatcaattg ttcccccgac
     4561 catccctacg tgaaggaaca cgaggaatgg ttcttcaaac ggccggacgg ctccatcaaa
     4621 tacgccgaga acccgccgaa gaaatacgag gacatctacc cgctgaactt ctatgcggcc
     4681 gacggccact ggcggcccct gtgggaggaa atgaagaaga tcctcgattt ctggatcggc
     4741 aaaggcgtgc gcatcttccg ggtggacaac ccccacacga agccggtgtt cttctggaaa
     4801 tggctgatcg aagacatcca acgggaacat cccgacgtgc tgttcctggc cgaagccttc
     4861 acccggcccc ccatgatgaa aatgctcgcc aaggccggct tctcccagtc ctacacttac
     4921 ttcacctggc ggcagttcaa gcaggagctc acggattacc acacggagct gacccagtcc
     4981 gaaatgaagg actatttccg cggcaacctg ttcgccaaca cccccgacat cctgccgctc
     5041 atcctccagg aaggcggccg gcccgcgttc aagtcgcggt tcgttctggc ggccacgctg
     5101 tcgtcggtgt acggcatcta cagcggcttc gagctctgtg agaacaaggc cctgccgggc
     5161 aaggaggagt acgcggactc ggagaaatac gagttcaaag cgcgggattg ggacaaaccg
     5221 ggcaacatca aggacctcat cgccaaggtg aaccgcatcc gccgagagaa ccccgccctg
     5281 caacattaca agaacctcag gttctaccgg accgacaacg acaacgtcct cttctacggc
     5341 aaagccaccg acgacaaaaa aaacgtcgtt ctggtggcgg tgaacctgga tccctaccgc
     5401 gcccaggaga ccaccctcca catccccatc gcggagttcg gcatcaaacc cgacgagacc
     5461 tatcaactcc aaaacctgct cgccggagac cgcctcctct gcaagggcga caagatcatc
     5521 gtgcggctcg acccgaaaga cgaaccggcc cacatctacc aactcaaccg ttggatgaag
     5581 agagaacagg acttcgatta tttcggaatg taatcccgtc cttcgataac aaaaagaaag
     5641 cccgtccccg cttgcgcaag cggggacggg ccttctcgtt tatcggacgg ccgcaggcgg
     5701 aacgacggag aggccggtct tatttcccct tcttgccggg tcggtacgtt ttttccgcca
     5761 tccgggcgta ctccagaatc gccttcaact gcccctcggc atggaccccc gcctcttcca
     5821 acaccttgtg cgcctgcccg ctgcccaggt aatggttccc gcagaaagag tgcagcgtgc
     5881 gtttcaaccc ctctttggac cgcacccatt gatacatcgt cggcagggta aagtccgtga
     5941 tgcccatggc ctcgtaggtc cgctccgtgg gcattatttt ttcctgctcc ttgtccgaca
     6001 gcagcccgaa caattccgcg ctggcgacgt agtagacgtt catgttcacg ccttttttat
     6061 cnnnnnnnnn ntcacaaaca cgctcgccac gccgttgccc tgcagaacca ccgtgccgtt
     6121 gtactgcttg gccttggggt cggcggtccg gatggcgtaa acgcccttgg cggccacgtg
     6181 ggcgggcggc aggccgagcg ccttgcggtc cggcacgctc tccgccggac gggtcacgaa
     6241 gggacagatc accgccggac gcgccttcaa ggcggcgatc atcagcggcc aaatctcctg
     6301 cgggtcccac ggcgtcagcg tgatcacgaa ccctttcgga aaattctcct gcaggagctg
     6361 cagcggctgc ggatcggcgt gcgtgggccc gtcctccccg gtcttcacgc cggcgtgggc
     6421 gttgatcatg atgaaaggct tgaagggttc tcccgtcaat tgtttgcggg actgctggcc
     6481 gatgctgtgc agccgcgcgg cgatgtgctc cagcgccgcg atgaacgcgg cgtaggacga
     6541 gatggcgccg atgtggttgc cgaaactggc caaccccgcc atcaccgcgc ccatgccgtc
     6601 ctcgcagatg ccgccgagcg aaagcgcccg cgagccgggg tttttctccg cgttgtaaaa
     6661 gccctgggga aatcccttgt tgatgttgct tacgctggtg gagcccatca ggtccgccga
     6721 ggcggcgaag atgccgccgc cgctgatctt gttcagcgcg ttcagggtgt cgcccatcac
     6781 gtcgcgcagg gtgatgctct tgccggggtc cggcgtcagg ccggccggcg gggtttccgg
     6841 cttcacgtcg ctcttgtaga tttcgccgat ccggggagca tccgcccgca cggtgcggct
     6901 ctgtttgtcc agccgcgcct tggagtcggc gatgcaacgg ccgagatacg ccgtggtctc
     6961 cttctccttc tccagcacgg cgcggaacac gagcagggtg tcgtagaaac atttttccgt
     7021 ttccgcctcc accgccgggt tcccggagaa ccgagggaac gacacggaga acgcgtcctc
     7081 gaactctttc agcgtggcgt aataaccgtc ggaacaaaac ttgtgtcccg ccccgtggga
     7141 cgaccgcccc tcgatgccgt aacgccaccc cttcacggtg cggtacacca cggcggtggg
     7201 catcttgttg gtcagggaat tcgccagttt ctgcgccgca aagacctgcc ggaaatcctt
     7261 gccgtccggc acatagatca cgttccaatc gtgcagatag gccagttcca tgggcgtcca
     7321 ctgaacgtag tcccccggct gcccgttctc ggcgcagacg tggttggagt cgatggaagc
     7381 ctgattccag tccacgtgca tcaccacgtt gtagagctgg gaggtggcgc aagcggccat
     7441 cgcctcgtgc acgcggccgg gcgtcatgcc gccctcgccc tccaccatgt gaacccaagg
     7501 cgccttggcg ccgtaagtat ccatggcgcc gaaagccaac ccgatggagg cgcccacgcc
     7561 cacaccgctg gcgccggtgg cgatgcggat gaacggggtg agcggggtgg gatggccgtc
     7621 cagcgctttc gacttc
//
LOCUS       DAOTFD010000119         7619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6817_length_7619_cov_4.338049, whole genome shotgun sequence.
ACCESSION   DAOTFD010000119 DAOTFD010000000
VERSION     DAOTFD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6817_length_7619_cov_4.338049"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3540
                     /locus_tag="P5079_07485"
     CDS             <1..3540
                     /locus_tag="P5079_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY29866.1"
                     /translation="VYGTSFTFSWSGGGNPGVTPYFVDVSTRADYSPIGYSSETTSAS
                     LSLTGLAGETTYYARVWARNGEGVYTSTVSLSTATQGVAPGVVSTLTTAQIPGHGVHL
                     AWSAPGDDDDAGTAAYYSLRYSSTVIITDEATWLNASVATAAPLSVTLPVPSSAGTPQ
                     QMDITNLLVDSTYYWSLRAVDELGQMSPVYATAVSTWITNLVPSAPTTVSPGNVVGST
                     VTYSTITATDITWGAAADPDADAVTYTLEYTTNNGVSWVPLTSTPTASPYAWPLPPDE
                     TNEARLRIRTVDPYAAASGWTESTYNFAIVVPDTIAPQAVNDFAASPGTADGTVNLFW
                     TSTGDDGNTKSLSPGSKFVIEHTTNAAYVAWSTTTAQVVISTSGVGPGASLAYTVSSG
                     LLAGSSHYFRLWAMDEAGNPGALSNGATAQAMDAIPPSVGVVEPPHTGYRNTLTALSG
                     TALDTSPGSGLSRVRIQLIDNSNGQYWNGGVWGGAAYLSTTSFAGDVWGYNGGLPTLS
                     VHGTSYTVHAVADDYWGRVTTATTRFTADLQAPAKINNLSALNNGYSGQITLTWTVPS
                     DDTGDGTLSPASYRVAFSSTANFVPGAFDAQTLRGLNNSGNVGNTHSYIVTGLTDGAT
                     YYFRIKSLDAAGNGSAVSDEVTAPNAFPGDLIGPNIVTTLATPVNVSPVGSVRLTWSV
                     PRDDGPYPVPASFDVRASTSAITNANFAAIGNSTVIAIDSTTVGVGDTVTATVTGLTV
                     GKLYYFGIKSYDYFGNVSGINDPIQALSIASDPAAPAAISPLRAETGSTEGAVKLAWS
                     VPNDDDGDPASGPPLTYDIRYATGPFGEAAFGSLSASSRLVSNGGASVGSEVSFSVTG
                     LTPGGTYYFRIKSIDDVNQTSPVDLTSPQAQAVAKDLADTAIKGNVTQANGTPIHLVL
                     VEAMAVGSETVLASARSNAGGEWRLDGLTAGALYTVRVSWTVDEVTSSAFRENIRSGT
                     SGVNFNLEINIQLATINGTVALARSAVPSGAYGAPVAGVGAAYVEIYQQGRKIGEIST
                     DVQGRYVVPNLLPGRYAVRAFNGLSYSELQDVTLAEGQVLTVQFKFDLLPEDKVYFYP
                     NPARNQVVIRFESSVQPLEAQALVFDIAGALVREFPGSESVRVGAEVSWTWDLSNAQG
                     EPLASGVYLVQVKVHDPATNRRTGIIKKLAVIR"
     gene            3606..4877
                     /locus_tag="P5079_07490"
     CDS             3606..4877
                     /locus_tag="P5079_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRY29867.1"
                     /translation="MKGKTTTALFLLFAITLAPHSAAWAARLASPQALVTDSAGASRT
                     SYSSTERITLSVKVQNVSPGAGSIVFTFIVRDPNGSQRLFQTGNSAPATVTGQAGASL
                     RGVPVSQFYTTPGNYTLAVRAVLDGETAEASAQFTVLSPVITLAYPPDGARDLIDQPL
                     VFRWVSSGATKYRISVDDDASFYNALFMADSFSSLFTYPSNPPDIKQRLAGGQVYYWK
                     VEGMDFAGNVVARTQIPFSFSLKTQAMQSNSRDLAVVGLEKVNENIPGMPGALPIAVT
                     VKNQGGKTEANIPVNLFADGASVPSSPKKLNSLEPGRTERLVFPARPPEAGRTLVVKA
                     ILDFFDDNVQNNSMTVQFQGEERRTRRLTPAEMWDILRRFISDPQIVGELEGYSCSEV
                     SSQGMSQNELSELVESVRGGKSRVGSAGLTP"
     gene            4895..5944
                     /locus_tag="P5079_07495"
     CDS             4895..5944
                     /locus_tag="P5079_07495"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRY29868.1"
                     /translation="MRKKFWVGSAALLFFAAAPLFARDVAGVITQIAGAVYLKEAGSL
                     SYRQAKKGEFVYEGTTLKTGRGDRAAISFVSGTEVRINEETTYIIRPTHPSRRGQGND
                     TSLKQGRMWFKVLRKQSKFQIRTPVAAVSVRGTEGDVNYSQGNMHAACYEGSFEVSGR
                     SGEDGDEGDTGGDDASGDGGSPGVTVGVGQLAVVTQGNPPEVRTYERKDNWQDRFPSG
                     GKGTIKLVPEKTEADRKAAFKITLTAYDAGNRKDTSLNAGVTLYADHDDIEFSATGQS
                     WARGPYGHRVSEGAAEFWVRGTKPGYVNVNAQTEGYDGVPARFMIAKPPQRELNVTVE
                     TEKGETKQLKLKFHR"
     gene            5997..>7619
                     /locus_tag="P5079_07500"
     CDS             5997..>7619
                     /locus_tag="P5079_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29869.1"
                     /translation="MIPVRRRSDGWFRCSVRLAAAGFFLFCGARLRAADTGQFQLLLA
                     APDPVVAGEQVQFQVIAINQGTEIWRARRFYFQAEVYDANRKYVGQTERLRGMRDVNP
                     GESAVSSLSFSFPVTASGKFYYRMYLVNEDQRIMESDFKPFTVRERAILLPRPPPVAL
                     GGNIVASYRNESGPNDYVGNLSVNMVGRYKDRSFLFNAYTISDRGDPIDFYTVLLNYY
                     GPQVTAGLGDVSPAFSPLSLYGQGMRGGTAETKWQAGWLGGGITLVGARTATAEEGSS
                     TTDGTFRRMLYGAKNEFYLPGRVTLRMNYVNTLDVQQSLDIPGPTLRPVDDRLAGGGL
                     AWEAVPGLKFEGEYQSSSYEADKLSTAPAVTDGAWRGAVGWESDRMNFNGYVQRTGPD
                     FVALGAPNATKDRFTYDAALSVSPFHWMSAYGSFNKYRDNLDDDPAVVTTHQQIVNSG
                     LSFNLPSMTGLNFGYSVNTARGEPRTAQDNQTLTTSFGVSQTWIGQTLFVTYQTSDFE
                     DKTQSSVAFSSGSKTNTDTYGTSLNLSFGSRVT"
BASE COUNT         1382 a         2403 c         2506 g         1328 t
ORIGIN      
        1 gtgtacggca cgagtttcac cttcagttgg agcggaggcg gtaatccggg ggtgacgccg
       61 tatttcgtcg atgtgtccac tcgggcggat tatagcccca tcgggtattc ctcggaaacc
      121 acctcggctt ctctgtcgct caccggcctt gcgggtgaaa cgacctatta tgcccgcgtg
      181 tgggcccgga acggcgaggg ggtctacacc tccacggttt ctctgtccac cgcgacgcaa
      241 ggcgtggcgc cgggcgtggt ttccacgctg accacggccc aaatccccgg ccacggcgtg
      301 cacctggcct ggtcggcccc cggcgacgac gacgatgccg ggaccgcggc ttattacagc
      361 ctgcggtatt cctccacggt gatcattacg gacgaggcca cctggctgaa cgcgtccgtg
      421 gccaccgcgg ctccgctgtc ggtaacgttg cccgtgccgt cctcggccgg aacgccgcaa
      481 caaatggaca tcaccaactt actggtcgat tccacctatt actggtccct gcgggccgtg
      541 gacgagctcg gtcagatgag cccggtctat gccacggcgg tgagcacctg gatcaccaac
      601 ctggttccgt ccgcgcccac gacggtttcg ccgggcaacg tggtgggctc caccgtcacc
      661 tacagcacca tcacggccac cgatatcacc tggggggcgg cggccgatcc cgacgccgac
      721 gcggtcacct acaccctcga atacaccacg aacaacggcg tttcctgggt gcccctgacc
      781 agcacgccca ccgcctctcc ctacgcctgg cccctgccgc cggacgagac caacgaggcg
      841 cggctgcgga tccggacggt ggacccctac gcggccgcct cgggctggac ggaatccacc
      901 tataatttcg ccatcgttgt tcccgacacc atcgcgcccc aggcggtgaa cgatttcgcc
      961 gcctcgccgg gaaccgccga cggcaccgtg aaccttttct ggacgtcgac gggggacgac
     1021 gggaacacca agagtttgtc gccggggtcc aagttcgtga tcgaacacac caccaacgcc
     1081 gcctatgtgg cgtggagcac cacgaccgcg caggtcgtca tctccaccag cggcgtcggg
     1141 ccgggggcgt ccctggccta cacggtttcg tccggtcttt tggccggatc cagccactat
     1201 ttccggctct gggcgatgga tgaggcgggc aatcccggcg cgctctccaa cggcgccacc
     1261 gcccaggcca tggacgccat cccgccgtcc gtcggcgtgg tggaaccgcc ccacacgggg
     1321 tatcggaaca cgttgacggc gctgtccggc acggcgttgg acacctcgcc ggggtccggc
     1381 ctgtcgcgcg tgaggataca gctcatcgac aacagcaacg gccagtattg gaacggcggg
     1441 gtctggggtg gggcggcgta tcttagcacg acatcgtttg cgggcgatgt ctggggttac
     1501 aacggcggtc ttccgacctt aagcgtgcac ggcaccagtt acaccgtcca tgccgtggcg
     1561 gacgattatt ggggccgcgt caccaccgcc accacccggt tcacggccga tcttcaggcc
     1621 ccggccaaga tcaataattt atccgcgttg aacaacgggt attcgggcca gatcaccctc
     1681 acctggacgg tccccagcga cgacaccggc gacggcacgc tgtcgccggc ctcctaccgg
     1741 gtggcgttct cctccacggc gaacttcgtt cccggcgctt tcgacgccca gacgctgcgc
     1801 ggtttgaaca acagcggcaa cgtggggaac acgcacagtt acatcgtcac cggtcttacc
     1861 gacggcgcca cgtattattt ccgcataaag tcgttggacg cggcgggcaa cggaagcgcc
     1921 gtcagcgacg aggtcacggc gccgaacgcg ttccccggcg accttattgg gcccaacatc
     1981 gtgaccaccc tggccactcc ggtgaacgtc agcccggtgg gttcggtgag gctgacctgg
     2041 tcggtgcccc gggacgacgg tccctatccc gtgccggcga gtttcgacgt gcgcgcctcg
     2101 acctcggcca tcaccaacgc caatttcgcg gccatcggca attcgacggt catcgcgatc
     2161 gattcgacga ccgtgggcgt gggcgacacc gtcacggcca cggtgacggg gctgaccgtc
     2221 ggaaaactgt attattttgg catcaagtcc tacgactatt tcgggaacgt cagcggcatc
     2281 aacgatccca tccaggcgct gtccatcgcc tccgacccgg cggcgccggc ggccatctcg
     2341 ccgctgcgcg cggagacggg ttccaccgaa ggggccgtca agctggcttg gtccgtgccg
     2401 aacgacgacg acggggaccc ggcctccggg ccgccgctga cctacgacat ccgttatgcc
     2461 accggcccgt tcggcgaggc ggcgttcggt tcgctgtcgg ccagttcccg gctcgtgtcc
     2521 aacggcggcg cctcggtggg gagcgaggtg tcgttctccg tcacggggct caccccgggc
     2581 gggacctatt atttccgcat taaatccatc gacgacgtga accagacttc tccggtggac
     2641 ctgaccagtc cgcaggcgca ggccgtggca aaagacctgg cggacaccgc catcaagggc
     2701 aacgtgacgc aggccaacgg cacgcccatc cacctggtgc ttgtggaggc catggcggtg
     2761 gggtccgaaa ccgtgctggc ctcggcccgg tccaacgccg gcggcgaatg gcgtttggat
     2821 ggcctgaccg ccggggcgct ttacaccgtc cgggtcagct ggacggtgga cgaggtgacc
     2881 tccagcgcgt tccgggagaa catccggtcc ggcaccagcg gcgtgaattt caacctggaa
     2941 atcaacatcc agctggccac catcaacggc acggtggcgt tggcccggtc ggcggtcccc
     3001 tcgggggcgt atggcgcccc ggtggcgggg gtcggcgccg cctatgtgga gatctatcag
     3061 caggggcgca agatcggcga gatctcgacc gacgttcagg gacggtacgt cgtccccaac
     3121 ctgctccccg ggcgctacgc ggtgcgcgcc ttcaacggcc tgagctacag cgaactgcag
     3181 gatgtgacgt tggccgaggg acaggtcctc acggtgcagt tcaagtttga cctgctgccg
     3241 gaagataagg tttactttta tcccaacccg gcccgaaacc aggtggtaat ccgttttgaa
     3301 agttccgtgc agccgctgga ggcgcaggcc ctggtgttcg acatcgcggg ggcgctggtt
     3361 cgtgagttcc cggggagcga atcggtgcgg gtcggggcgg aggtttcctg gacgtgggac
     3421 ctctccaacg cgcaggggga accgctggcc tcgggcgtgt atctggtcca ggtgaaagtc
     3481 catgacccgg ccacgaaccg tcgaaccggg atcatcaaaa aattggcggt catccggtag
     3541 tgaaacggtg gaagggtgaa atgggaaacg gacaaaaaaa cagtgtaaag tcgaaggtcg
     3601 gagggttgaa gggaaaaaca acgacggcgt tgtttttact cttcgctatt acactcgctc
     3661 cccattccgc ggcctgggct gcgcgtttgg cctcgcccca ggccctggtg acggattcgg
     3721 cgggagcctc ccggaccagt tattcctcca cggaacggat caccctttcg gtaaaggtgc
     3781 agaatgtttc gccgggcgcc ggaagcatcg tgttcacgtt catcgtgcgc gatcccaacg
     3841 gcagtcaacg cctgtttcag acaggcaact cggccccggc gaccgtgacg ggccaggccg
     3901 gcgcctcgtt gcggggcgtg ccggtgtcgc agttctacac cacgccgggg aactacacct
     3961 tggccgtgag ggcggtcttg gacggcgaga ccgcggaggc ctccgcgcaa ttcacggtgt
     4021 tgagtccggt catcaccctg gcctatccac ccgacggcgc gcgcgacctc atcgaccagc
     4081 ccctggtgtt ccggtgggtt tcttccgggg cgacgaaata ccggatctcc gtggacgacg
     4141 acgcctcttt ttacaacgcc cttttcatgg cggattcgtt ctcgtcgctc ttcacctacc
     4201 cctcgaaccc gccggacatc aagcaacgcc tggcgggggg gcaggtctac tactggaaag
     4261 tggagggaat ggatttcgcc ggcaacgtgg tggcccgcac gcagatcccg ttcagtttca
     4321 gcctgaagac gcaggccatg caatccaact cgcgcgatct ggccgtggtg ggattggaaa
     4381 aggtgaacga gaacatcccg gggatgcccg gcgccctccc catcgcggtg acggtgaaga
     4441 accagggggg gaagaccgag gccaacatcc ccgtgaacct gttcgccgac ggcgcctccg
     4501 tgccgtcctc gcccaagaag ctcaacagtc tcgagccggg caggacggaa cggctggtgt
     4561 tcccggcgcg gccgccggag gccgggcgga ccctggtggt gaaagccatc ctggatttct
     4621 tcgacgacaa cgtgcagaac aattccatga cggtccaatt tcagggggag gaacgccgga
     4681 cgcggcggct gacgccggcg gagatgtggg acatcctgcg ccgattcatt tccgatcccc
     4741 agatcgtggg cgagttggag ggttattcgt gctccgaagt ttcctcccag gggatgagcc
     4801 agaacgagct gagcgagctg gtggaatcgg tgaggggcgg caaatccagg gtggggtccg
     4861 cggggctgac gccgtgagag tggggacgat gacgatgcgg aaaaaatttt gggtggggtc
     4921 ggcggccttg ctgtttttcg cggcggcgcc gctgttcgcg cgggacgtgg cgggcgtgat
     4981 cacgcagatc gccggggcgg tgtatttgaa ggaggccggg agcctttcct atcggcaggc
     5041 caaaaaaggc gagttcgtgt atgagggcac caccctgaag accggccgtg gcgaccgggc
     5101 ggccatctcg ttcgtgagcg gcaccgaggt gcggatcaac gaggagacca cgtatatcat
     5161 ccggccgacg cacccgtccc gccgcgggca ggggaacgac acgtcgttga aacagggccg
     5221 tatgtggttc aaggtgttgc ggaaacagag caaattccag atacgcaccc cggtggcggc
     5281 ggtgtccgtc cgcggcacgg agggcgacgt gaactattcg caggggaaca tgcacgcggc
     5341 ctgttacgag gggagtttcg aggtgagcgg acgttccgga gaggacggag acgaggggga
     5401 cacgggggga gacgacgctt ccggggacgg agggtcgccg ggcgtgacgg tcggcgtcgg
     5461 ccagctggcg gtggtgaccc agggcaatcc gccggaggtg cgcacctacg aacgcaagga
     5521 caattggcag gaccgtttcc cgtccggcgg aaaagggacg atcaagctgg ttccggaaaa
     5581 aaccgaggcg gaccgcaagg cggcgttcaa gatcacgctg accgcctatg acgccggcaa
     5641 tcgcaaggac acctcgttga acgccggcgt gacgttgtat gccgaccacg acgatattga
     5701 gttttccgcc acgggccaga gctgggcgcg ggggccttac ggccatcgcg tgagtgaagg
     5761 ggccgccgaa ttttgggtcc gggggacgaa acccgggtac gtgaacgtca atgcgcagac
     5821 ggaggggtac gacggcgttc ccgcgcgctt catgatcgcc aagccgccgc agcgggagct
     5881 gaacgtaacg gttgaaacgg aaaaagggga aacaaaacag cttaaattga agttccaccg
     5941 ataaaccaac acgattgaag aacaacacgg agctgcccac acgacaaaag aatgatttga
     6001 ttccggtccg acgccgttcg gacggatggt ttcgctgttc cgtccgtctg gccgcggccg
     6061 gttttttcct tttttgcggg gcgcgcctgc gcgccgcgga taccgggcag ttccaactgc
     6121 ttctggccgc gcccgacccg gtggtggccg gcgaacaggt gcagttccag gtcatcgcca
     6181 tcaaccaggg gacggagatc tggcgcgccc ggcgtttcta ttttcaggcg gaggtctacg
     6241 acgccaaccg gaaatacgtg gggcagaccg aacgcctccg cgggatgaga gacgtcaacc
     6301 ccggggagtc ggcggtctcc tccctgtcct tcagttttcc cgtcaccgct tccgggaagt
     6361 tctactaccg catgtatctc gtcaacgagg accagcgcat catggagagc gatttcaagc
     6421 cgttcacggt ccgcgagagg gccatcctcc ttccgcggcc gccgcccgtg gccctggggg
     6481 gtaacatcgt ggcctcctat cgcaacgaaa gtggccccaa cgactatgtg ggcaaccttt
     6541 ccgtcaacat ggtcggccgc tacaaggacc ggtcgttcct tttcaacgcg tacaccatca
     6601 gcgatcgcgg ggaccccatc gatttctaca ccgtgctgtt gaactattac gggccccagg
     6661 tgacggcggg gctgggggac gtgtccccgg cgttttcgcc gttgtccttg tatgggcagg
     6721 gcatgcgggg cggcacggcc gaaaccaaat ggcaggccgg ttggctcggc ggggggatca
     6781 ccctggtggg cgcccggacg gcgacggcgg aggaaggcag ttccacgacg gacggaacgt
     6841 tccgtcggat gctctatggg gccaagaacg agttctatct gccgggccgc gtgacgttgc
     6901 ggatgaacta tgtgaatacg ttggacgtgc agcagtcctt ggatattccc ggcccgaccc
     6961 tgaggccggt ggacgaccgt ctggcgggcg gcggtttggc gtgggaagcg gtccccggtt
     7021 tgaagttcga gggggaatac cagtccagtt cctacgaggc cgacaagctc tccacggcgc
     7081 ccgcggtgac ggacggcgct tggcgcgggg cggtgggctg ggagagcgac cggatgaatt
     7141 tcaacggcta tgtgcaacgg acgggcccgg atttcgtcgc cctcggcgcc cccaacgcca
     7201 ccaaggaccg gttcacctac gacgccgcct tgtcggtgtc gccgttccac tggatgagcg
     7261 cctacggcag tttcaataaa taccgggaca atctggacga cgacccggcg gtggtcacca
     7321 cgcaccagca gatcgtgaac agcgggttgt cgttcaacct gccgagcatg accgggctga
     7381 acttcggcta ttccgtcaac acggcccggg gcgaaccacg gaccgcccag gacaaccaga
     7441 cgctgacgac ctccttcggg gtgtcgcaga cttggatcgg ccagacgctt ttcgtgacgt
     7501 atcagacctc cgacttcgaa gacaagaccc agtccagcgt ggctttcagc tccggttcga
     7561 agaccaacac cgacacctac ggcaccagcc tcaacctgtc tttcggcagc cgggtgacc
//
LOCUS       DAOTFD010000120         7590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_6866_length_7590_cov_4.086397, whole genome shotgun sequence.
ACCESSION   DAOTFD010000120 DAOTFD010000000
VERSION     DAOTFD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6866_length_7590_cov_4.086397"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5079_07505"
     CDS             complement(<1..642)
                     /locus_tag="P5079_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRY29870.1"
                     /translation="MTPKQAELQKMGQDYQYGFSDPTDKYVFQSAKGLTRGIVESISD
                     LKDEPAWMREFRLKSLDIFLKKPMPTWADTEALNTIDFDNIHYYMKPAERQSRDWNDV
                     PESIKRTFDRLGIPEAERKFLAGVTAQYESEVVYHSIRKDLEKLGVVFLGMDEGLKKH
                     PDLVKKYFGTIIPPADNKFAALNSAVWSGGSFVYVPRGVNVAIPLQAYFRINSE"
     gene            complement(674..1444)
                     /gene="sufC"
                     /locus_tag="P5079_07510"
     CDS             complement(674..1444)
                     /gene="sufC"
                     /locus_tag="P5079_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRY29871.1"
                     /translation="MTDTTPLFQIKDLHVRVEGKKILNGVTLTVKPGEIHAVMGPNGS
                     GKSTLAYTAMGHPRYVVESGEVLLRGENILSLKPHERAIRGLFLAFQYPVAVPGVSVA
                     NFLRTAMKAVRKQDIAVKEFRKELKEKMAWLDMASSFASRYLNDGFSGGEKKRLEILQ
                     MALLSPSMAILDETDSGLDIDALRIVSDGINRVASPERGMLVITHYQRLLNYVKPHVI
                     HVLAGGRIVRTGGPELALLLESKGYEGIEQEAAQGASA"
     gene            complement(1459..1953)
                     /locus_tag="P5079_07515"
     CDS             complement(1459..1953)
                     /locus_tag="P5079_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY29872.1"
                     /translation="MKITAMQEYGLRCILQLAARKPSTSHMTVREIAKQEHLTPVYVA
                     KILVTLRRGGLVKSIRGVNGGYALSRPPREISAARVLAVLGKVDLGKNLCKRFPGMSS
                     HCTHSGDCRIRPLWYVLTQYIYNFLDRLTLDQLIQEEAGVSREIRRLQNQNPLPAPKT
                     PALV"
     gene            2118..3302
                     /locus_tag="P5079_07520"
     CDS             2118..3302
                     /locus_tag="P5079_07520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY29873.1"
                     /translation="MKIKKMQDLDLKNKRVLVRVDYNVPTKDGRVTDDTRIQASLPTL
                     RHLLAAGARVILMSHLGRPKGQVTPKYSLSPVAKHLGQVLGNPVEFASDCLGPAAASA
                     AQNLPPGGILVLENLRFHPEEEKNDENFAKSLASLGEVFVQDAFGAVHRAHASTSAVT
                     RFLPSGAGFLLQKELQFLEQIREKPAKPYVALVGGAKVSDKIEVLEKLLAKLDTLAIG
                     GAMAYTFLSAMGVPVGKSLVEKDKTDLAKSLLDKAQKKNVTILLPRDHMVVKAIEDEA
                     SAKYTDDQAIPEGLIGVDVGPKTVQRLLAPIGSAKTIFWNGPMGIFEVARYSKGTTDV
                     AKLAAEAAGRGTVVVIGGGDSVAAVQAAGLADKITHISTGGGASLEFLEGKDLPGVAA
                     LA"
     gene            3326..3781
                     /gene="secG"
                     /locus_tag="P5079_07525"
     CDS             3326..3781
                     /gene="secG"
                     /locus_tag="P5079_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY29874.1"
                     /translation="MQAVVLTLHVIVCLLVILVVLIQSGKGAGLSGVFGGGGGDALFS
                     APSGSSFLRKLTTSLAVAFFLTSLTLTYLAARRGFRTVTRGPWGQGQRIPVQPGQPLV
                     PGMPMMPAVPAAPGQVAGPTAGAANVPTVPVKGKTVQKNKTQSPAAPKK"
     gene            3814..3895
                     /locus_tag="P5079_07530"
     tRNA            3814..3895
                     /locus_tag="P5079_07530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3848..3850,aa:Leu,seq:gag)
     gene            4057..4602
                     /locus_tag="P5079_07535"
     CDS             4057..4602
                     /locus_tag="P5079_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29875.1"
                     /translation="MKNFLILAALAILGSVLYFKFLAVPYGISSAAPLGDFDKLDSYL
                     TQKLNLEKQPLQTLDLPGAEPSDRMFEYADTQQKYQIRETVILFTDKAGGLQGVSGAF
                     VTPPAHDHQYRSKIQRFMRKYWDKVAGGIPVFTPTAFEREKKRLVGARADFVVGPVRG
                     TWIKSNEALTLQFISLRRKEK"
     gene            complement(4661..5413)
                     /locus_tag="P5079_07540"
     CDS             complement(4661..5413)
                     /locus_tag="P5079_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRY29876.1"
                     /translation="MKSTAPSSFSFERWRGWGRRALQFFFPRTCAACRNDVPLSSPYA
                     LCADCWSKVPVWEGLICETCGVPLPDGGRHCYLCRRIRRSFHTCRSAALYEGVMKTCL
                     WLLKYQGKDFLAEPLGRFLAEKCSSFPEMSGVEGVLPVPLYFWRKRRRGYNQSELLAT
                     VFCRETSFPLMTGVLRRRKPTRTQTELGREERFANVENAFEVEKPENVRGKNLLLIDD
                     VCTTGATLDACARALKTAGARRVGALTVARQI"
     gene            complement(5379..>7590)
                     /locus_tag="P5079_07545"
     CDS             complement(5379..>7590)
                     /locus_tag="P5079_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29877.1"
                     /translation="GQIKVGVRNTTGAPLSSEVWVNEIHLSTARQKVGVAKRFSGDFT
                     LPKWATFGGTYKKVDRNFQTLTSPVTNQDRTDDSAYLNFNRLSILPLTFSGSRSETIT
                     PAAVRTGDSALVSVLEEGRVTTRQGSGKGELILPYLPTLGFGYEKSITDSNLLKRIDD
                     KDTYSGSLNYTLPFRLDLMPTRYATLRPLPDSVSLTYRRTNYFLSFYPEKKEEELSVS
                     SGNVTTQKNAIFANVRTVEFSDDWSGRASFTPWAGLSINPNYAWKKVKEQRRFTEKDL
                     NIAPDFRAAKDYDKSLSQTQGVSASWRLLRWLEPRFSYSVSGTETNVLPSVSSPTAYN
                     LKSLDRTADGEASWTFSARELIPNFRPVQSLTINNSFRIQDGDTYENVGRTFTDWQKI
                     QLVQLQRIPTVSTGAVASTGTVAGKSTKVKRQSGRPIFGLLRPLDIRNPEAFRKQVTI
                     RNTYRSSGNWSPLDWLSVPRLLQPIKTFRLTATVTNTDEHSETTETIRDAVTIIWPDL
                     IYSLRDTEKFFHLERWMTNSQANLRTNRKRTETFKVQFTQEDSLTTDYRFTLWRRYDL
                     FSSYGRTDSFDRDLRTNVLNSVGRGHNYSTQVGMTFGSWRFTPSAAYSTSFSFDGAGK
                     TLTDLTTRTYGLKSRFDKSYPDGFRMPFSRRVFGKVNRFTVDSNLTFERKQSSLNVER
                     DNTDVYTASLSGEFEIAQNFRWSLGSAGSYNKNREKKDENIITYEINSTLVIQF"
BASE COUNT         1359 a         2211 c         2471 g         1549 t
ORIGIN      
        1 ttccgagttg atacggaaat acgcctgcag ggggatggcc acgttgacgc cccggggcac
       61 gtacacgaac gagccgcccg accacaccgc cgagttgagc gcggcgaact tgttgtcggc
      121 cggcgggatg atggtgccga aatatttctt caccaggtcc gggtgtttct tcaggccttc
      181 gtccatgccc aggaagacca cgcccagctt ttccaggtcc ttccggatgg agtggtagac
      241 cacctccgac tcgtattggg cggtgactcc ggcgaggaat ttccgttccg cctccgggat
      301 gcccagacgg tcgaaggtgc gtttgatgct ttccggcacg tcgttccagt cgcggctctg
      361 acgctcggcg ggtttcatgt agtagtggat gttgtcgaag tcgatggtgt tgagcgcctc
      421 ggtgtcggcc caggtgggca tgggtttttt caggaagatg tccagggatt tgaggcggaa
      481 ctcccgcatc cacgccggct cgtccttgag gtcggaaatg gactcgacga tgccccgcgt
      541 caatcctttg gcggattgga agacatattt gtccgtcggg tcgctgaacc cgtactggta
      601 gtcctgcccc atcttctgca actcggcctg tttgggcgtc atgggcccct ctttttctgt
      661 cccctccggc aatttaggcc gacgcgcctt gcgccgcctc ctgttcgatc ccttcgtatc
      721 ctttcgattc cagaagcagc gcgagttccg gtccgccggt gcgcacgatg cgtccgccgg
      781 ccaggacgtg gatgacgtgc ggcttcacgt agttcagcag ccgctggtaa tgcgtgatca
      841 ccagcatgcc ccgttccggc gaggccacgc ggttgatgcc gtcggaaacg atccggaggg
      901 cgtcgatgtc cagccctgag tccgtctcgt ccaggatggc catggacggc gacaggagcg
      961 ccatctgcag tatctccagg cgcttcttct cgccgccgga aaaaccgtcg ttcaagtaac
     1021 gggaggcgaa ggaggaagcc atgtccagcc acgccatctt ctccttcagt tccttgcgga
     1081 actctttgac ggcgatgtcc tgtttgcgga ccgccttcat ggccgtacgc aggaagttcg
     1141 ccacgctcac ccccggcacc gccaccggat attggaacgc cagaaagagg ccccggatcg
     1201 cccgttcgtg cggtttaagg gaaaggatgt tctctccccg taaaaggacc tctcccgact
     1261 ccacgacata gcggggatgg cccatcgcgg tgtaggccag ggtgctcttg ccggaaccgt
     1321 tgggacccat cacggcgtgg atctcgcccg gcttgacggt gagggtgacc ccgttgagga
     1381 ttttcttccc ctccacgcgc acgtggaggt ctttgatctg gaaaagcggg gtggtgtcgg
     1441 tcacgagggg tctccttctc aaaccagcgc cggcgtcttg ggcgccggca acgggttttg
     1501 gttctgtagt cgccggattt cacgggaaac accggcttct tcctggatga gttggtccag
     1561 cgtcaggcga tcgaggaagt tgtaaatgta ttgggtcagc acgtaccaga gcggccggat
     1621 acggcagtcg cccgagtggg tgcaatggga ggacatgccg gggaaccgtt tacagaggtt
     1681 cttgccgaga tccactttgc cgaggaccgc cagcacccgg gcggcggaga tttcccgggg
     1741 cggccggctc aacgcatagc cgccgttgac gccgcggatg cttttcacca gaccgccgcg
     1801 ccgcagcgtc accaggatct tggcgacgta gaccggcgtg aggtgttcct gcttggcgat
     1861 ctcccgcacc gtcatgtgcg aggtcgaggg cttgcgcgcg gccaactgca ggatgcagcg
     1921 cagaccgtac tcttgcatgg cggtgatttt catggggtcc ccgtccttgt ttgaggattt
     1981 ttatggtgcg tctcctcagt ttatcgaaat atgacaaatt agtcaagtat cggcgtccgg
     2041 gtcgggaagc tttacaggaa cgccgtttct ttatagaatc cttcccttct attgtcatct
     2101 ccaaggagcc ggttctcatg aagatcaaga agatgcagga tttggacctc aaaaacaagc
     2161 gcgttctggt gcgcgtggat tacaacgtcc ccaccaagga cggtcgcgtg accgacgaca
     2221 cccgcatcca ggcctccctc cccactctcc ggcatctcct ggccgccggg gcgagggtca
     2281 tcctcatgtc ccacttgggt cggcccaaag ggcaggtgac cccgaaatat tccctgtccc
     2341 ccgtggcgaa acatctcggg caggtcttgg gcaacccggt ggagttcgcc tcggactgtc
     2401 tcggccccgc cgccgcgtcc gccgcccaga acctcccgcc gggtgggatc ctcgtgcttg
     2461 agaatctccg gttccatccg gaagaggaga agaacgacga aaatttcgcc aaatccctgg
     2521 cgtctctcgg cgaggtgttc gtgcaggacg ccttcggcgc cgtgcatcgc gcccacgcca
     2581 gcacctcggc ggtcacccgg ttcctcccct ccggcgccgg attccttttg cagaaagagc
     2641 tgcagttcct ggaacagata cgcgaaaaac ccgccaaacc gtacgtggcc ctggtgggcg
     2701 gcgcgaaagt gtccgacaag atcgaggtgc tggaaaaact cctcgccaag ttggacaccc
     2761 tcgccatcgg cggcgccatg gcctatacgt tcctctccgc catgggcgtt cccgtcggaa
     2821 agtccttggt ggagaaagac aagaccgacc tggccaaatc gcttttggac aaggcccaga
     2881 agaaaaacgt gacgatcctc ctgccgcgcg accacatggt ggtgaaggcc atcgaggacg
     2941 aggcttcggc caagtatacc gacgaccaag ccatccccga ggggttgatc ggtgtggacg
     3001 tcggccccaa gaccgtgcag cgccttctgg cgccgatcgg ttccgccaag acgatcttct
     3061 ggaacgggcc catggggatt ttcgaggtgg cgcggtattc caaaggcacc accgacgtgg
     3121 ccaagctggc ggcggaggcg gccggccgcg ggaccgtggt ggtcatcggc ggcggcgaca
     3181 gcgtggccgc ggtgcaggcc gccggcctgg ccgacaagat cacacacatc tccacgggcg
     3241 gcggcgcgtc gttggagttc cttgaaggca aggacctccc cggcgtcgcg gcgctggcct
     3301 agaccggaca gggacggagg aaaccatgca agcggttgtt ttgacgttac acgtgattgt
     3361 ctgtttattg gtgattctgg tggtccttat ccagtcgggc aaaggggccg ggttgtcggg
     3421 ggtgttcggc ggcggaggag gcgacgctct cttctcggcg ccgtcgggat cgtctttcct
     3481 tcggaaactc accaccagcc tggcggtggc ttttttcctg acgtcgttga cgttgaccta
     3541 tctggccgcg cggcggggat tccggacggt cacgcggggc ccgtggggcc agggccagcg
     3601 gattcccgtg caacccggcc agcccctggt tccgggaatg ccgatgatgc cggccgttcc
     3661 ggcggccccg gggcaggtgg ccggacccac ggcgggcgcg gccaacgtgc cgacggtgcc
     3721 cgtcaagggc aagaccgtcc agaaaaacaa gacgcaaagc ccggcggcgc ctaaaaagta
     3781 aagatttttt cccgcctttt cagtgcaaac cgtgcgcggg tggcgaaact ggcagacgcg
     3841 tacgtttgag gggcgtatgg ggtaaccctt gcgggttcaa gtcccgcccc gcgcacggtt
     3901 tgcataccga gatactccaa aagacattct gttcaaaatc aaatcgaccg ggggccgccc
     3961 ggggagcgat atttttgtgt tgatttaagg agggaatgga cttaaactta aagcagcggg
     4021 tctatgggga tatgagagtc aggaaagagg ccggacatga agaattttct gatactcgcc
     4081 gccctggcga tcctgggatc tgtgctctac ttcaaattcc tggcggtgcc ttacggcatc
     4141 tcctccgcgg ctcccttggg cgatttcgat aaactggatt cctacctcac gcagaaactg
     4201 aatctggaaa aacaaccgct tcaaaccctg gacctccccg gcgcagaacc ttccgaccgg
     4261 atgttcgaat acgccgacac gcaacaaaaa tatcaaatcc gtgagacggt gatacttttt
     4321 accgataaag ccggggggct tcagggggtc agcggcgcct tcgtcacccc tccggcccac
     4381 gaccaccagt accgctcgaa gatccagcgg ttcatgcgga aatattggga taaggtggcc
     4441 ggagggatcc ccgtttttac gcccacggcg ttcgaacggg aaaagaaacg cctcgtcggc
     4501 gcgcgtgcgg atttcgtcgt gggccccgtc agggggacct ggatcaaaag caacgaagca
     4561 ctgaccctgc aattcatttc cctgcgccgg aaagaaaaat aagtttcaaa tccccgccca
     4621 tcagttccgt attcgaaacc tctcccccac gtgcaagacg ttatatctgg cgggccacgg
     4681 tcagcgcgcc gacgcgccgg gccccggccg tttttaaggc gcgggcgcag gcatcgagcg
     4741 tggccccggt ggtgcagacg tcgtcgatga ggagcaggtt tttccccctc acgttttccg
     4801 gtttttcaac ctcgaaggcg ttctccacgt tggcgaatcg ttcctcgcgg ccgagttccg
     4861 tctgtgtgcg cgtcggtttc cgtcgacgca ggacccccgt catcaaaggg aaggaggttt
     4921 ctcggcagaa aacggtcgcc agcagttccg attggttgta ccccctccgg cgctttcgcc
     4981 aaaaatagag cggaacgggg agcaccccct ccaccccgct catctccggg aaagaggaac
     5041 atttttccgc caggaaccgg ccgagcggtt cggccaggaa atccttcccc tgatatttca
     5101 ggagccagag gcaggtcttc atcacgcctt cgtagagggc ggcgcttcgg caggtgtgaa
     5161 aggaccggcg gatccgccgg cagagataac agtgcctccc cccgtcgggc aggggaacgc
     5221 cgcaggtctc acagatgagg ccttcccaca ccggcacctt ggaccaacag tcggcacaga
     5281 gggcgtacgg cgaactcagc ggcacatcgt tccggcaggc cgcacaggtc ctgggaaaga
     5341 aaaactggag cgcccgccgg ccccagcccc gccatcgttc aaaactgaat gacgagggtg
     5401 ctgttgattt cataggtgat gatgttttcg tccttcttct cgcggttctt attgtaactg
     5461 ccggcgctgc cgagggacca gcggaagttt tgggcgatct cgaactcgcc cgacaggctg
     5521 gcggtgtaca cgtcggtgtt gtcccgctcc acgttcaggg acgattgttt gcgttcaaac
     5581 gtgaggttgg aatccaccgt gaaacggttc accttgccga acaccctccg gctgaacggc
     5641 atgcggaagc cgtcgggata ggatttgtcg aagcggctct tcaggccgta ggtccgggtg
     5701 gtgaggtcgg tgagggtctt tccggccccg tcgaaggaga aactggtgct gtaggcggcg
     5761 ctgggcgtga agcgccagga gccgaaggtc atgcccacct gcgtggagta gttgtgcccg
     5821 cgccccacgc tgttcaggac gttggtgcgc aggtcgcggt cgaaactgtc ggtgcggccg
     5881 taactggaga aaagatcgta ccggcgccag agggtgaaac gataatcggt ggtcagggag
     5941 tcttcctggg tgaactgcac cttgaacgtc tcggtgcgtt ttcggttggt gcggaggttc
     6001 gcctgggagt tggtcatcca gcgttcgagg tggaagaact tctccgtgtc gcgcagggag
     6061 tagatcaggt cgggccagat gatggtgacg gcgtcgcgga tggtctcggt ggtttcggag
     6121 tgctcgtcgg tgttggtgac ggtggcggtg agacggaagg tcttgatcgg ctgcaacagt
     6181 cgcggcacgg agagccagtc gagcgggctc cagttgccgc tggaacggta ggtgttgcgg
     6241 atggtcacct gtttccggaa ggcttcgggg ttgcggatgt ccagcggccg gagcaggccg
     6301 aagatgggcc ggccgctctg gcgtttcacc ttggtggatt ttcccgccac cgtgccggtg
     6361 ctggcgacgg cgccggtgct gaccgtgggg atgcgctgca gttggacgag ctggattttt
     6421 tgccaatccg tgaacgtcct ccccacgttt tcataggtgt cgccgtcctg gattcggaaa
     6481 ctgttgttga tggtgagcga ctgcaccggc cggaagttgg ggatgagttc ccgcgcgctg
     6541 aaagtccagg aggcctcgcc gtcggcggtg cggtcgaggc ttttgaggtt gtaggccgtg
     6601 ggcgaggaca ccgacggcag gacgttggtc tccgtgccgg agacggaata gctgaagcgc
     6661 ggctccagcc agcgcagcag ccgccaggag gcggagaccc cctgggtctg ggaaagggat
     6721 ttgtcgtagt ccttggcggc gcggaaatcc ggggcgatgt tcaggtcctt ctccgtgaag
     6781 cgccgctgtt ccttgacctt cttccaggcg tagttcgggt tgatgctgag gccggcccag
     6841 ggcgtgaaac tggcgcgtcc cgaccagtcg tcgctgaatt cgacggtgcg cacgttggcg
     6901 aagatggcgt ttttctgcgt ggtcacgttc cccgaagaga cgctcaactc ctcctctttc
     6961 ttctcgggat agaacgacag gaaataattc gtccgccgat aggtgaggga gacgctgtcc
     7021 ggcaggggcc gcagcgtggc gtagcgggtg ggcatcaggt ccagccggaa cggcagggta
     7081 tagttcaaac tgccgctgta ggtgtccttg tcgtcgatgc gcttgaggag gttggagtcg
     7141 gtgatggatt tctcgtaccc gaacccgagg gtgggcaggt aggggaggat gagttctccc
     7201 ttgccgctgc cctggcgcgt ggtgacgcgg ccctcttcga gcacggagac caacgccgag
     7261 tcccccgtgc ggaccgcggc cggggtgatg gtttcggacc ggctgccgct gaaggtgagc
     7321 ggcaggatgc tcaggcggtt gaaattcaga taggccgagt cgtcggtgcg gtcctggttg
     7381 gtgacgggcg aggtgagcgt ttggaagttg cggtccactt ttttgtaggt gccgccgaag
     7441 gtggcccatt tcggaagggt gaagtctccg gagaaacgct tggccacgcc gactttctgg
     7501 cgcgccgtgg agaggtggat ttcgttgacc cacacctcac tggagagggg cgcgcccgtg
     7561 gtgttgcgca ccccgacctt gatttggccg
//
LOCUS       DAOTFD010000121         7462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7017_length_7462_cov_4.266235, whole genome shotgun sequence.
ACCESSION   DAOTFD010000121 DAOTFD010000000
VERSION     DAOTFD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_7462_cov_4.266235"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="P5079_07550"
     CDS             <1..267
                     /locus_tag="P5079_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRY29878.1"
                     /translation="RRPEDQGRAGRPQLSDLRESGAIEQDADVVAAIFREEVYKRDDP
                     DLKGKAKLFILKQRNGPIGDVDLNFLEEYTLFVDPAPSEDEPFS"
     gene            284..1459
                     /gene="alr"
                     /locus_tag="P5079_07555"
     CDS             284..1459
                     /gene="alr"
                     /locus_tag="P5079_07555"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY29879.1"
                     /translation="MTVPSPETNPLPSAASFFRPTWADIDVAAFRHNLRTMAGCLAPG
                     VKTIVVLKANGYGHGAVPLARAAAAESGVSLWGFGVSSIEEGLLLRESGFRHPVLILG
                     SLFPFENFVPALEHDLIPAVASLAAARALGGIATERGRRADVHVKVDTGMGRIGVSPQ
                     AAAQIVDAVLENRSLHLEGIFTHLAHAESEEDTQRQLDVFQAVAEEARRKKGEPLLAH
                     AANSAATFLYPRSHGDAVRPGLSLYGIYPNESLRARVSLQPVMTWKTRIVFLKTVKGG
                     TAVSYGATFRTSRPSRLATLPVGYADGYRRILSNRAFVLVKGRRCPVLGRVTMDQIVV
                     DVTDVPAVDVGEEVVLLGRQGKDAVTAEELAGWAETIPYEIVCGVSVRVPRRYVNVS"
     gene            1486..2274
                     /locus_tag="P5079_07560"
     CDS             1486..2274
                     /locus_tag="P5079_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29880.1"
                     /translation="MFHNPVAFVGRRLLGWARTLGGVVVLVQKTVYWLFKARPDVRNT
                     AIQMNEIGVRSFPVTSLTALFTGMVLALQTGYSFRKVFNEPLYVGTVVGLSLLKELGP
                     VLTALVVAGRVGAAIAAELGTMNVTEQIDALYTLGTNPVRYLVVPRFLACLVMVPLLT
                     VYADLIGILGGFGIAHWRLGIPAPVFWDEINAIGLEDGFHGLIKSVAFALIIVITACY
                     KGIRCQGGAEGVGQATTSTVVVSMVMVLVSDYFLSSLLVSLGVG"
     gene            2271..3032
                     /locus_tag="P5079_07565"
     CDS             2271..3032
                     /locus_tag="P5079_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29881.1"
                     /translation="MIRLQGVHKAFGANRVLRGVDLEIPEGETLTIIGGSGTGKSVTL
                     KLMVGLLRPDHGRIWVDDTETTFLNEDKLFEEQKKFGFLFQGAALFDSLTVAENILFG
                     VRNLRPELLANARGVVEQKLGFVGLKPEVALLKPAELSGGMKKRVGLARAIAHEPKYI
                     LYDEPTTGLDPIMSDVINELILSLQERLKVTSIVVTHDMKSTYKVSNRIAMLHEGRIV
                     AEGTPREIQSSENPLVKQFITGSSQGPIQMAVRHA"
     gene            3029..4387
                     /locus_tag="P5079_07570"
     CDS             3029..4387
                     /locus_tag="P5079_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY29882.1"
                     /translation="MNVETRVGAFTMAGLAVFAGGILILGDVQFRDRYDLNVYFQNAE
                     GLPDKGPVKVAGVEVGKVKKITLEGGRARVEVSLDTDVKVHADAEASIAATGLIGTKY
                     LDLSLGSPEAPLLKEGDTLQGQPGFSFDDVMKELRQFFKEDPEDGSLSENFRRTMANL
                     RRVTDSLNVALGQQGKELTEIVQNIRSLSEHAKNVSANLDEVTEQHKADLKTMLTKFR
                     SVSERLDDLLAKVQNGEGTMGKLVNDEQMGKDLKQTFTDVRQAAKEAQAILGRIAMID
                     VYWDYRQRYDFEDNQYRADLGLRIVPRPGKFYFIQGNNLGAREDRKLAGNDIERKNTI
                     TGVMGKEFGPFTLYGGAIRSTAGGGMRFRPLFMSQKWNRRFELEAEAYNFGRDEVVQG
                     LKMDDPVYSAGARFLVADPWLWVGGQVEDIAERKNFNANLNVTFKDEDVAYLLGLVGL
                     AN"
     gene            4404..4919
                     /locus_tag="P5079_07575"
     CDS             4404..4919
                     /locus_tag="P5079_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRY29883.1"
                     /translation="MMREARIYSLAQVGGQCVLVLEEVDGPRLLPIWIGLYEGGSIGM
                     ALAGQKFPRPLTHDLLLEAVGQLGAEVEKIVITELKESTFYAQIHVKRDKEAIVLDAR
                     PSDSVALAVRQPCPLYIDEKVFEACPELLKPISEDEVQDFKKKLESMTPEDFFRDLKK
                     KNEGEGGGAEK"
     gene            4923..6299
                     /gene="radA"
                     /locus_tag="P5079_07580"
     CDS             4923..6299
                     /gene="radA"
                     /locus_tag="P5079_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRY29884.1"
                     /translation="MKTKGASRTVFRCQNCGDAQPKWLGQCPSCNQWNTLVEEKEAPP
                     DRPARRLTEFSSAVTKLSDVTVEKAQRKVTGLGEFDRILGGGVWPGSMVLLGGAPGIG
                     KSTLMMQVAGRLASRDYPVLYVSGEESLGQVKGRATRLNIVNDAVLLLSETNLENILE
                     VVRQNQPKALVLDSIQTVYKSDLAGAPGSVGQVRECAAELLHLAKGIGITVFVLGHVT
                     KEGDLAGPRVLEHIVDTVLYFEAERQQVHRILRAFKNRFGPTNEIGVFEMTGGGLAEV
                     SNPSELFLGEHEAGVPAGTAVIAAMEGTRPLLVEVQSLVSRTLFGMPRRQVAGVDYNR
                     AALLIAVLDKRCGLNLESQDVYVNVTGGLQVREPSADLGIAVAIASACLEKPAPPKTV
                     WLGEVGLGGELRAVPQLGDRLQEAAKLGFTRAVVPRAALKGVRPPSGLELSPVVTLTE
                     ALEFVSRR"
     gene            6346..7386
                     /locus_tag="P5079_07585"
     CDS             6346..7386
                     /locus_tag="P5079_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29885.1"
                     /translation="MLINLIRLLVIIVGPVIGYMQISPDAKGILIGVAAAVLIIAVEI
                     VIDKVALDALISGVIGAIVGLVSAQLLNWVVYQMDNPRIYELLQKYSLLIHVVFAYLG
                     LAIFVRKRSELELLDRDLIVKGSKKKLSNTHVVDTSVLIDGRIADVCETKFLGGTLVV
                     PRFVLSELQHIADSSDSNRRARGRRGMEILSRLQENPDVPVKIFDKDFADIKEVDSKL
                     VALSKDLGAKILTTDFNLNKIASLQGVVVLNINDLANALKPVVLPGESMSVFVVKEGK
                     EREQGVGYLDDGTMVVVEDGRRFIGHKLNVMVTSILQTSAGRMIFTKSKDRAPAPSQE
                     TPAPSGPPPAEG"
BASE COUNT         1328 a         2249 c         2518 g         1367 t
ORIGIN      
        1 cgccgtccgg aggaccaggg ccgggcgggc cggccccagc tttccgatct gcgggaatcc
       61 ggcgccatcg agcaggacgc cgacgtggtg gccgccattt ttcgcgagga ggtctacaag
      121 cgggacgacc cggacctgaa aggcaaggcc aaacttttca tcctcaagca acgcaacggt
      181 cccatcggcg acgtggactt gaacttcctc gaggaataca cgctcttcgt ggatccggcg
      241 ccgtccgagg acgagccgtt ttcctgaacg acggacgccg ggcatgacgg ttccttcgcc
      301 ggaaacgaat ccgttgccgt ccgcggcgtc ttttttccgg cccacctggg ccgacatcga
      361 cgtggcggcg ttccggcata acttgaggac gatggccggc tgcctggccc ccggcgtgaa
      421 gacgatcgtg gtgttgaagg ccaacggtta cggtcacggg gcggtgccgc tggcccgcgc
      481 ggccgcggcg gagtccggcg tttccttgtg gggcttcggc gtctccagca tcgaggaggg
      541 gcttctgttg agggagtccg gtttccgcca tccggtactc atcctgggga gcctgttccc
      601 gttcgagaat ttcgttccgg cgttggagca cgacctcatc ccggcggtgg ccagcctggc
      661 cgcggcgcgg gcgttggggg gcatcgccac ggaacgcggc cgtcgcgccg acgtgcacgt
      721 gaaggtggac accgggatgg ggcgcatcgg cgtttcgccg caggccgccg cccagatcgt
      781 ggacgcggtg ctggaaaacc gctcgctgca cctggaaggg atctttaccc atttggccca
      841 cgcggaatcc gaggaggaca cccagagaca gctcgacgtg ttccaggcgg tggcggagga
      901 ggcccgacgg aagaaggggg agcctctcct ggcccacgcc gccaactcgg ccgccacgtt
      961 cctgtatccg aggtcccacg gcgatgcggt acggcccggc ctgtcgttgt acggcattta
     1021 tccgaacgag tcccttcggg cgcgggtgtc cctgcagccc gtgatgacgt ggaagacgcg
     1081 gatcgttttt ttgaagacgg tgaagggcgg aacggcggtc agttacggcg ccacgttccg
     1141 caccagccgc ccctcccgct tggccaccct gcccgtggga tatgccgacg gataccggcg
     1201 gattctttcc aaccgcgcct tcgtgctggt gaagggccga cgctgccccg tgctggggcg
     1261 ggtgaccatg gaccagatcg tcgtggacgt gaccgacgtg ccggccgtgg atgtgggcga
     1321 ggaggtcgtg ttgctgggtc ggcaggggaa agacgccgtg acggcggagg aactggccgg
     1381 ctgggcggag accatcccct atgagatcgt ctgcggggtg tcggtgcgcg tgccgaggcg
     1441 ctacgtgaac gtttcatgaa agggcgaatg tgaagaggaa ccgccgtgtt tcataatccc
     1501 gtcgcgttcg tcggccgccg cctgctggga tgggcgcgga cgttgggcgg ggtcgtcgtc
     1561 ctcgtccaaa agacggttta ttggctgttc aaggcccgtc cggacgtgcg caacacggcc
     1621 atccagatga acgagatcgg ggtgcgttcc ttcccggtca ccagcctcac ggcgctgttc
     1681 accggcatgg tgctcgcctt gcagaccggg tattcgttcc ggaaggtttt caacgagccg
     1741 ctctacgtgg gcaccgtggt ggggctgtcg ctcctaaagg aactgggccc ggtgctgacg
     1801 gcgctggtgg tggccggccg ggtgggcgcg gccatcgccg ccgagctcgg caccatgaac
     1861 gtgacggaac agatagacgc cctgtatacc ttgggcacca accccgtgcg gtacctggtg
     1921 gttccgcggt tcctggcgtg cctggtgatg gtgccgctgt tgacggtgta cgcggatttg
     1981 atcggcatcc tcggcgggtt cggcatcgcc cactggcggc tggggatccc cgccccggtc
     2041 ttttgggatg agatcaacgc catcgggctg gaggacgggt tccatggatt gataaagtcc
     2101 gttgcttttg ctctcattat cgtcatcacc gcttgttaca aggggatacg ctgccagggc
     2161 ggcgccgagg gcgtgggtca ggcgaccacc agcacggtgg tggtgtccat ggtgatggtg
     2221 ctggtgagcg actacttttt gtcgtcgttg ttggtgtcct tgggcgtcgg atgatccgcc
     2281 tgcagggggt gcacaaggcg ttcggcgcca accgcgtgtt gcgcggggtg gacctggaga
     2341 tcccggaggg ggagaccctc accatcatcg gggggtccgg caccggcaaa agcgtgaccc
     2401 tgaaactgat ggtgggcctg cttcggccgg accacgggcg catctgggtg gatgacacgg
     2461 aaaccacctt cctgaacgaa gacaagctct ttgaagaaca gaagaagttc gggttcctgt
     2521 tccagggggc ggcgctgttc gactcgttga cggtggcgga gaacattttg ttcggcgtgc
     2581 ggaacctccg gccggaactc ctggcgaacg cgcgcggcgt ggtcgaacag aagctggggt
     2641 tcgtgggttt gaaacccgag gtggcgctgc tgaaaccggc ggagttgtcg ggcggcatga
     2701 agaaacgcgt cggcctggcc cgcgccatcg cccacgaacc gaaatatatt ttatacgacg
     2761 agccgacgac cgggctggac cccatcatgt cggacgtgat caacgaactg atcctctcgc
     2821 tgcaggagag gctgaaggtg acgtccatcg tggtcacgca cgacatgaag tccacctaca
     2881 aggtgtccaa ccggatcgcc atgctccatg agggccgcat cgtggcggag gggacgccgc
     2941 gggagatcca gtcttcggag aatccgttgg tgaaacagtt catcacgggg tcgtcccagg
     3001 gccccataca gatggcggtg aggcacgcat gaacgtcgaa acaagagtag gcgcttttac
     3061 catggccggc ctggctgttt tcgccggcgg catcctcatc ctcggcgacg ttcaatttcg
     3121 ggaccggtac gacctcaacg tctatttcca gaacgccgag gggctgccgg acaagggccc
     3181 cgtgaaggtg gccggcgtgg aggtgggaaa ggtcaagaag atcacgctgg aggggggacg
     3241 ggcccgcgtg gaggtgtctc tggacacgga cgtgaaggtg catgccgacg ccgaggccag
     3301 catcgccgcc accggcctca tcggcacgaa atacctggac ctttccctcg gctctcccga
     3361 ggcgcccctc ctcaaggagg gggacaccct tcagggccag cccggcttca gtttcgacga
     3421 cgtgatgaag gagctgcggc agtttttcaa ggaggacccg gaggacgggt cgctcagcga
     3481 gaatttccgc cggaccatgg ccaacctccg ccgcgtgacc gattccttga acgttgctct
     3541 ggggcagcag ggcaaggaac tgaccgagat cgtccagaac atccgcagcc tctccgagca
     3601 cgccaagaac gtgtcggcca acctcgacga ggtcaccgag cagcacaagg ccgacctgaa
     3661 aacgatgctg accaaattcc gctccgtttc cgagcggttg gacgaccttc tggcgaaagt
     3721 ccagaacggc gaagggacga tggggaaact cgtcaacgac gagcagatgg ggaaagacct
     3781 caagcagacc ttcaccgacg tgcgtcaggc ggcgaaggag gcccaggcta tcctcggccg
     3841 catcgcgatg atcgacgtgt attgggacta tcgccagcgc tacgatttcg aggacaacca
     3901 gtaccgcgcc gacctcggtc tgcgtatcgt gccgcggccg ggaaaattct atttcatcca
     3961 ggggaacaac ctcggcgcgc gggaggaccg caagctggcc ggcaacgata tcgaacgcaa
     4021 gaacaccatc accggcgtga tgggcaagga gttcgggccc ttcaccctct acggcggcgc
     4081 catccgctcc accgccggcg gcgggatgcg tttccggccg ttgttcatgt ctcagaaatg
     4141 gaaccggcgt ttcgagctgg aggccgaggc ctacaatttc ggccgcgacg aggtggtcca
     4201 gggcctcaag atggacgacc ccgtctactc ggccggcgcg cgtttcctgg tggcggaccc
     4261 gtggctgtgg gtgggtggtc aggtggagga tatcgccgag cgcaagaatt tcaacgccaa
     4321 cctgaacgtg acgttcaagg acgaggacgt cgcctatctg ttggggctgg tcggtctcgc
     4381 caattgacgg ggaggggaac cgcatgatga gggaagcgcg catttattcg ttggcccagg
     4441 tcggcggcca atgcgtcttg gtgctggagg aggtcgacgg cccgcggctg ttgcccatct
     4501 ggatcggact ttacgagggc ggctccatcg gcatggccct ggccggacag aagtttccgc
     4561 gcccgctcac ccacgacctg cttttggaag cggtggggca gctcggcgcc gaggtggaga
     4621 agatcgtcat cacggaattg aaggagagca ccttttacgc gcagattcac gtcaagcggg
     4681 acaaagaggc catcgtcctc gacgcccgcc cgtcggattc cgtggccctc gccgtccgcc
     4741 agccgtgccc gctgtacatc gacgagaagg ttttcgaggc ctgccccgaa ctgttgaagc
     4801 ccatctccga ggacgaggtt caggacttca agaagaagct ggagtccatg acgccggaag
     4861 attttttccg tgacttgaag aaaaagaacg aaggggaagg cggcggggcg gaaaaataac
     4921 gcatgaagac gaagggcgcc tcccgcaccg tttttcgttg ccagaactgc ggcgacgccc
     4981 agcccaaatg gctgggacag tgcccttcct gcaatcagtg gaacaccctg gtggaggaaa
     5041 aagaggctcc ccccgaccgg ccggcccggc ggttgaccga gttctcctcc gccgtcacca
     5101 agctgtccga cgtgacggtg gaaaaggccc agaggaaggt caccgggttg ggcgagttcg
     5161 accggatcct cggcggcggc gtgtggcccg ggtccatggt gcttctcggc ggcgcgccgg
     5221 gcatcggcaa gtccaccctg atgatgcagg tggcgggccg gctggcctcc cgcgattacc
     5281 cggtgctcta cgtttccggc gaggagtcgc tgggccaggt gaaggggcgc gccacccgcc
     5341 tgaacatcgt caacgacgcg gtgctgctgt tgtcggagac gaacctggag aatattttgg
     5401 aggttgtgcg gcagaaccag ccgaaggcgc tggtgctgga ttcgatccag acggtgtaca
     5461 agtcggacct ggccggtgcg ccgggctcgg tgggccaggt ccgcgaatgc gccgccgaac
     5521 tgctgcacct ggccaaaggg atcggcatca cggtcttcgt tctgggacat gtgaccaaag
     5581 agggggatct cgccgggccg cgcgtcctgg agcacatcgt ggacacggtc ctctatttcg
     5641 aggccgagcg ccagcaggtg caccgcatcc tccgggcttt caagaatcgg ttcgggccga
     5701 ccaacgagat cggcgtcttc gagatgaccg gcggcggcct ggcggaggtt tccaaccctt
     5761 cggaactttt tttgggggaa cacgaggccg gcgttcccgc gggcacggcg gtgattgcgg
     5821 ccatggaagg cacccgcccc ttgttggtgg aggtgcagtc gctggtctcc cgtacgcttt
     5881 tcggaatgcc ccgccggcag gtggcggggg tggattacaa ccgggcggcg cttttaatcg
     5941 ccgtcctgga caagcgctgc ggtttgaacc tggaatcgca ggacgtgtac gtcaacgtca
     6001 ccggcggact gcaggtccgt gaaccgtccg ccgacctcgg catcgcggtg gccatcgcca
     6061 gcgcctgtct ggagaaaccc gcgccgccca aaaccgtttg gctgggcgaa gtggggctgg
     6121 gcggggaact ccgcgccgtt ccgcagctcg gcgaccggct tcaggaggcg gcgaaactgg
     6181 gtttcacgcg ggctgtcgtc ccgcgggcgg cgttgaaagg ggtccgtcct ccgtccggat
     6241 tggagctttc ccccgttgtg acgctcacgg aagcgttgga gtttgtatcc cggcgataga
     6301 tatcgggaag tccgggaact tttgaggaag agaaggtaat caatcgtgct cattaatctc
     6361 atacgtcttc ttgtcatcat cgtcggcccc gtcatcggtt atatgcagat atcccccgat
     6421 gccaagggca ttcttatcgg tgtagcggcg gctgtgttga tcatcgcggt ggagatcgtc
     6481 atcgacaagg tcgcgctgga tgccctcatc tcgggcgtca tcggcgccat cgtgggtctg
     6541 gtgtccgcgc agctcctgaa ctgggtcgtc tatcagatgg acaacccgcg tatctacgaa
     6601 ctgctgcaaa aatattcgct cctcatccac gtggtcttcg cctacctggg cctggccatc
     6661 ttcgttcgga aacgttccga gctggaactg ttggaccggg acctgatcgt caaagggtcg
     6721 aagaagaagc tttccaacac gcacgtggtc gacacctcgg tcctcatcga cggccgcatc
     6781 gccgacgtct gcgagacgaa attcctcggg ggcacgctgg tggtgccccg cttcgtgctc
     6841 tcggaactgc agcacatcgc ggattcctcc gactccaacc gccgcgcccg cggccggcgc
     6901 gggatggaga tcctttcccg cctgcaggaa aaccccgatg tccccgtcaa gatattcgac
     6961 aaggactttg cggacatcaa ggaggtcgac tccaaactcg tcgccctctc caaggacttg
     7021 ggcgccaaga tcctcaccac ggatttcaac ctgaacaaga ttgccagcct tcagggcgtc
     7081 gtggtgctca acatcaacga cctggccaac gccctgaagc ccgtggtgct ccccggcgaa
     7141 agcatgagcg tcttcgtggt gaaggagggc aaggagaggg aacagggcgt cggatatctc
     7201 gatgacggca ccatggtggt ggtggaggac ggccgccggt tcatcgggca taaactgaac
     7261 gtgatggtca cctccatcct gcagacctcc gccggccgga tgatcttcac gaaatccaag
     7321 gaccgggcgc ccgctccgtc acaggaaacg ccggccccgt ccggcccccc gccggcggaa
     7381 ggctgacccc cgccctttcc ggatgcctgt ttccgccgtc gttcttgcgg ccggccgggg
     7441 cgcccgcttc ggcggtccgc cc
//
LOCUS       DAOTFD010000122         7445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7043_length_7445_cov_4.720433, whole genome shotgun sequence.
ACCESSION   DAOTFD010000122 DAOTFD010000000
VERSION     DAOTFD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_7445_cov_4.720433"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="P5079_07590"
     CDS             <1..320
                     /locus_tag="P5079_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY29886.1"
                     /translation="EESQGTAVEVSDGEIFEAQGILARTAGVFAEPSAAAALAGLVAL
                     KKQGLVANKERVVCLVTGNGLKDVQSVLERLGEPPRVEPRWEAVQEVLGALSQKRDTA
                     VIE"
     gene            345..1274
                     /gene="argF"
                     /locus_tag="P5079_07595"
     CDS             345..1274
                     /gene="argF"
                     /locus_tag="P5079_07595"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRY29887.1"
                     /translation="MKKDLLSIEDLTQVEIVSLLESTRALKAQRGPVRDLQGKSLGMI
                     FQKPSTRTAVSFAVAMYELGGLPLFLNAQDLQLKRGESLGDTAKTLSRYLSGIMIRAN
                     KHSEVEELAKHATLPVINGLTDKEHPCQVLGDLFTILEVFKLKDPRQLKNYRVAYVGD
                     GNNVAQSWMLAAGLLGMELVVCSPEGYLPEKEFIARASAVCGKSGGKVLYEKDPKTAV
                     AGAAVIYTDVWASMGKDEEHDRRKQVFSSYQVNETLLQNAGKNAVVMHCLPAHRGEEI
                     TDGVMDGPRSVVFDQAENRLHVQKAVLLQYLKK"
     gene            1364..1873
                     /locus_tag="P5079_07600"
     CDS             1364..1873
                     /locus_tag="P5079_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRY29888.1"
                     /translation="MIRDLDGVRPEIHDAAFVHEAAVLVGRVRLGADSSVWPCAVLRG
                     DLEEIVVGDRTNIQDGAVLHTDRGYPTTLGSGITVGHGAILHGCRVKDNCLIGMGAVL
                     LNGCVVEEDSMVAAGALLPPGLRVPQGHLAVGVPAKVIRPLEREEVRRIHENAALYVE
                     NIKRYAARP"
     gene            1955..3013
                     /locus_tag="P5079_07605"
     CDS             1955..3013
                     /locus_tag="P5079_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29889.1"
                     /translation="MSNEAFLKNIEINDREMRERKIRLDSKPLLLGLILTNWCNLNCI
                     MCGKLRHSNSRTLSRQELAKIMELLPYLQSIEWQGGEFFQLEHVKEFFTGLQPFSQIK
                     HHITTNGLLLDPDWIELFFRLKTELFFSIDAPTASVYERIRRGGRFETLIRKLELLVG
                     REKRLGRTLRRTLGAVVMRSNYRCLAGFGDFAKEYGFQELVVSPVMQFEDEENIFLRA
                     DADVDKELAAARASLEEQFKGTPVALKWLLPARTGPVSTVDVPAAEGRKEAFFCPLPW
                     KSLFVCVDHRGDIFPDCWCTTPVGNIGVDGFLDVWNNEKMQAYRRGVAEGGMSLCSAR
                     CRAGHSVDMKARLVSPLL"
     gene            3039..3770
                     /locus_tag="P5079_07610"
     CDS             3039..3770
                     /locus_tag="P5079_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29890.1"
                     /translation="MKRPTAGTVAWVYRVLLKRFGPRGWWPVTPPGGGRPLYAKKNTT
                     GRLTAPQQFEVAVGTILTQNTAWTNVEKALVELGRAGLLSSRALRRAPLAKLYRAIRS
                     SGYFRQKSRRLRDFCRFLAVCWGDRLDRFLDRPTAIVRRELLGLKGVGPETADSILLY
                     AGRHPVFVVDAYTRRFGRRFGFFRTDDYQTVQGFFAERTRPAAAYYREYHALLVELGK
                     NYCRAAPRCAGCPLRLRCATGRPAR"
     gene            3778..5454
                     /locus_tag="P5079_07615"
     CDS             3778..5454
                     /locus_tag="P5079_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY29891.1"
                     /translation="MTTNNQSSEYLFSAIRGLEHRASALTQITEFVRCIEDIGQVLDK
                     LLKKVMELAGADAGVIALADSGSSEFKFIAAAWAGISPTEAAARENVFKAVRMDITEG
                     VIGQVYKTGDPQVLSKVSENSRFRKDISDLVRYEIKNLIAVPLTIDDRRLGVLELCNK
                     KPDGSAFTNEDLALVVALSNQIALVMEAHKLREQSGRQVNQLGLLLKSLEIVNSSLAL
                     DVVLDNLMGMGMQLIGAEAASILLMDEELGQLYFAAATGAKKEEMKRIYLKKGEGIAG
                     WVADRGEVLLIPDVSKDQRFSKKADKASGFMTKSILAVPLKTEGKLVGVAEAINKKDG
                     SEFNETDAQMLSTLASYGAMAIQKAQLYQDVSELFMATIRALADAIEAKDATTRGRAE
                     RIRKLAVALAEEMKLGEKETKDIELAALMHDVGKIAVPDSILNKNENLTEEEYRTLMR
                     VPMIGAEILSPIKQLRGAVPVIRHANERWDGKGYPDRLSGSNIPQGSRVLAVANTFDA
                     MTTDRPYRQGLPDDVALKEMASYAGVQFDPACVEAFIRAYRKGKFKGITR"
     gene            5474..5800
                     /gene="cutA"
                     /locus_tag="P5079_07620"
     CDS             5474..5800
                     /gene="cutA"
                     /locus_tag="P5079_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRY29892.1"
                     /translation="MDRFIVVLITASSSAESARLSKALVKERLAACVNIVPGLRSVYR
                     WKGRIESSAERLLIAKTRRSKLPQLIRRVRQLHSYSVPEIIALPMLAGHADYLRWIAA
                     SLVPEK"
     gene            5898..6407
                     /locus_tag="P5079_07625"
     CDS             5898..6407
                     /locus_tag="P5079_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HRY29893.1"
                     /translation="MEPIAEKDFVCRGDGAGRMVWEGKEYRDCPGGHLPAGGVRPELL
                     ALLNALQARVGAALVLTSGYRCEAHNRYSWACLARDGDAAGVSRRSLHRSGAAADFYV
                     EGVRDISKYRTFAEELRRTAAASPGKTVWTRVYGAEEVRDPDNRHDYPYIHVHLLGVP
                     RRPLEPASD"
     gene            complement(6404..>7445)
                     /locus_tag="P5079_07630"
     CDS             complement(6404..>7445)
                     /locus_tag="P5079_07630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY29894.1"
                     /translation="SFENTFRRLLPRVVLAAAFFHIAPPSLSASQAEIACVGDVMMAY
                     HLKPVLKRKGPHYPFDKIRALLKKSDFVFGNLENPIGTTGEEYPDKEYIFRMEPAHAA
                     ALWEAGFRVMSLANNHILDFGPEALAETGRHLYDQGIASCGAGKNIEEARKPAVVMVR
                     GKRIAFLAYSLTFPIAFSAGPSTPGTAFADEKLVREDIAHAKKYCTWVIVSFHWGEEY
                     TYLPSDLQRKLAHTAIDAGADAVIGHHPHVAQGLERYKKKIIAYSLGNFVFGTRNPNA
                     VEGLVLKLKLRTGHSPRAEILPVLVQNTKTGFQTRPLKKAALRKFIHRLKQLSAELDT
                     SLRVKKGRVLLP"
BASE COUNT         1372 a         2152 c         2492 g         1429 t
ORIGIN      
        1 tggaggagag ccaagggacg gccgtggagg tcagcgacgg ggagattttc gaggcgcagg
       61 gcatcctggc gcggacggcc ggcgtgttcg ccgaaccctc cgccgcggcg gccctggccg
      121 ggctggtggc cctgaaaaaa caaggtcttg tcgcgaacaa ggaacgcgtc gtctgcctcg
      181 tcaccgggaa cggactgaag gacgtccagt cggttttgga acgattgggc gagccgccgc
      241 gtgtcgagcc gcgctgggaa gcggtccagg aagtgctggg cgctctctcg caaaagcggg
      301 ataccgccgt catcgagtaa attcggtttt ttgaggaggt tgtcgtgaaa aaggatttat
      361 tgtccatcga ggatctgact caagttgaaa tcgtttccct gctggagtcc acccgcgccc
      421 tcaaggcgca gcgcggcccg gtccgggacc tgcaggggaa atccctgggg atgattttcc
      481 agaaaccgtc cacccgcacc gcggtgtcgt tcgccgtggc catgtacgag ctgggcggcc
      541 tgccgctctt cttgaacgcc caggacctgc agttgaagag gggtgaatct ctgggcgaca
      601 ccgccaagac cctgtcgcgc tacctgtcgg gcatcatgat acgcgccaac aagcattccg
      661 aggtggagga gctggccaaa cacgcgaccc tccccgtcat caacggactc accgacaagg
      721 aacatccttg ccaggtgctg ggcgatctgt tcaccatcct ggaggtcttt aaattgaagg
      781 acccccgcca attgaagaat tatcgggtgg cctacgtcgg cgacggcaac aacgtggccc
      841 agtcgtggat gctggccgcc gggctgctcg ggatggaatt ggtggtttgc tcgccggaag
      901 ggtatctgcc ggaaaaagaa ttcatcgccc gggcgtccgc cgtgtgcgga aaatcgggcg
      961 ggaaggtcct ctatgagaag gacccgaaaa cagcggtggc gggggcggcg gtgatctaca
     1021 ccgacgtgtg ggcgtccatg gggaaggacg aggaacatga ccggcgcaag caggtgttca
     1081 gctcctatca ggtgaacgag acgcttttgc agaacgccgg gaaaaacgcg gtggtgatgc
     1141 attgtctgcc ggcgcatcgg ggcgaggaga tcaccgacgg cgtcatggac ggcccccgct
     1201 ccgtcgtgtt cgaccaggcg gaaaaccgcc tgcacgtgca gaaggccgtt ctgctccagt
     1261 atttgaagaa ataaatcaaa cggatgtcgc atcccggcat cgagtttaat ttgtcccgag
     1321 gagaagtccc gacgagggat cgccgtcgcc cgcgaaggac cgtatgatcc gcgacctgga
     1381 cggtgtccgg ccggaaattc atgacgcggc gttcgtccac gaggcggctg tcctcgtcgg
     1441 ccgggtccgg ctcggggcgg attcttccgt gtggccctgc gcggtgctgc ggggggacct
     1501 ggaggagatc gtggtggggg accggaccaa catccaggac ggcgcggtgc tccacacgga
     1561 ccgcggttac ccgaccaccc tcggcagcgg catcacggtg ggccacgggg ccattttgca
     1621 cggatgccgc gtgaaggaca actgtctcat cggcatgggg gccgtcctcc tgaacggctg
     1681 cgtggtagag gaggattcca tggtggcggc cggggccctg cttccgcccg gtctccgcgt
     1741 gccccagggg cacctggcgg tcggcgttcc ggccaaggtg atccggcccc tcgagcggga
     1801 ggaggtgcgg cgcatccatg agaacgccgc cctctacgtc gagaacatca agcgatacgc
     1861 ggcccggccg taagaatttt cgcgggtttc actgttcttt aaaaatcttt tctgggccgt
     1921 cttttttcca gaagaaggaa tcgggcaacg gggaatgtcg aacgaagcgt ttctgaagaa
     1981 catagagatc aacgatcgcg aaatgcgcga acggaagatc cggctggatt ccaaaccgct
     2041 gctcctcggc ctgatcctga ccaactggtg caatttgaac tgcatcatgt gcggaaagct
     2101 ccgccacagc aactcgcgga cgctctcgcg gcaggaactg gccaagataa tggaattgct
     2161 cccgtatttg cagtccatcg agtggcaggg gggcgagttt tttcagctgg aacacgtgaa
     2221 ggaattcttc accgggctgc agccgttctc tcagatcaag caccatatca cgacgaacgg
     2281 cctgctgttg gacccggact ggatcgaact tttttttcga ttgaagacgg aactgttttt
     2341 ttccatcgac gcgccgacgg cttccgtgta cgagcgcatc cgccgcggcg ggcgttttga
     2401 aacgctgatt cggaagctgg agctgctggt gggccgggag aagcgtctcg gccggaccct
     2461 ccggaggaca ctcggcgcgg tcgtcatgcg gtccaactac cgatgtctcg ccgggttcgg
     2521 ggatttcgcg aaggaatacg ggtttcagga actggtcgtt tcgccggtga tgcagttcga
     2581 ggacgaggaa aatatttttt tgcgcgccga tgcggacgtc gacaaggagc tcgccgcggc
     2641 ccgcgcgagc ctggaagagc agttcaaggg cacgccggtc gccctgaaat ggttgttgcc
     2701 ggcccggacc gggcccgttt cgaccgtcga tgttccggcc gcggaggggc ggaaggaggc
     2761 gtttttctgt ccgctgccct ggaaaagcct tttcgtctgt gtggaccacc gcggggacat
     2821 ttttccggat tgttggtgca ccacgcccgt cggcaacata ggagtggacg gctttttgga
     2881 tgtttggaat aatgaaaaaa tgcaggccta ccggcgcgga gtagcggagg gggggatgtc
     2941 cctctgctcg gcccgctgtc gggccgggca ctcggtggac atgaaggccc ggctggtctc
     3001 gccgctgctt tgaatcgttc ggacgacgga gtgccgcgtt gaaaagaccg accgcgggga
     3061 cggtggcctg ggtctatcgg gttcttttga aaaggttcgg cccgcgcgga tggtggccgg
     3121 tgacgccgcc gggggggggc cgtccccttt acgcgaaaaa gaacacgacg ggccggctca
     3181 cggcgccgca acagttcgaa gtggccgtcg gaacgattct gacccagaac acggcttgga
     3241 cgaacgtgga gaaagcgctg gtggaattgg gccgcgcggg gcttttgtcc tcccgggccc
     3301 tccggcgcgc gccgttggcg aaactttacc gcgcgatccg ttcgtccggc tatttccggc
     3361 agaaatcgag gcgtctgagg gatttctgcc gtttcctggc cgtgtgctgg ggagaccgcc
     3421 tggaccggtt cttggatcgt ccgacggcga tcgtgcgtcg ggaacttttg gggctcaagg
     3481 gcgtggggcc ggagaccgcc gattccattt tgctttacgc cggccgccac ccggtgttcg
     3541 tggtggatgc gtatacgcgc cggttcggcc gccggttcgg ttttttccgg accgacgatt
     3601 accagaccgt tcaggggttt ttcgcagaac ggacgcggcc cgcggcggcg tattatcgcg
     3661 aataccacgc gcttttggtg gagttgggga aaaactattg ccgcgccgcg ccgcgctgcg
     3721 ccgggtgccc gctccggctc cggtgcgcta ccggccgccc ggcccgatga aaaatttatg
     3781 acgaccaaca atcagagcag cgaatacctt ttctccgcca tccgcgggct ggagcaccgc
     3841 gcctccgccc tgacccagat cacggagttc gtccgttgca tcgaggacat cggccaggtg
     3901 ttggacaagc tcctgaaaaa ggtgatggaa ctggccggcg ccgacgccgg cgtcatcgcc
     3961 ctggccgatt ccggcagcag cgagttcaag ttcatcgccg cggcctgggc cgggatctcc
     4021 ccgacggagg cggcggcccg cgagaacgtt ttcaaggccg tccgcatgga tattacggag
     4081 ggggtcatcg gacaggtcta caagaccggc gatccgcagg tcctctccaa agtctcggag
     4141 aattcccgtt ttcgaaaaga catctccgac ctggtcagat acgagataaa aaacctcatc
     4201 gccgtcccgc tcaccatcga cgaccggcgg ctgggcgtcc tggagctctg caacaagaag
     4261 ccggacggat cggcgtttac caacgaggac ttggcgttgg tcgtggccct gtccaaccag
     4321 attgcgctgg tgatggaggc ccacaagctg cgcgaacaat ccggccgcca ggtgaaccag
     4381 ctcggcctgc tgttgaagtc cctggagatc gtgaactcca gcctggcgct ggacgtcgtt
     4441 ttggacaatc tgatgggcat ggggatgcag ctgatcgggg cggaggcggc gagcatcctc
     4501 ctgatggacg aggagttggg gcagctctat tttgccgccg ccacgggcgc caagaaggag
     4561 gagatgaaac ggatctatct gaaaaaaggc gaggggatcg ccgggtgggt cgcggaccgg
     4621 ggcgaggtgc tgctcatccc cgatgtttcc aaggaccagc gtttctccaa aaaggcggac
     4681 aaggcctcgg ggttcatgac gaaatccatt ttggcggtcc cgctcaagac ggaggggaaa
     4741 ctggtcggcg tggcggaagc catcaacaag aaggacggca gcgagttcaa cgagaccgac
     4801 gcccagatgt tgagcacgct ggcgtcctac ggcgccatgg ccattcaaaa ggcgcagctc
     4861 tatcaggacg tgagcgaact cttcatggcg accatccggg ccctggcgga cgccatcgag
     4921 gccaaggacg ccaccacccg cggccgtgcg gaacggatac ggaaattggc cgtggcgctc
     4981 gccgaggaga tgaaactggg cgaaaaagag acgaaggaca tcgaactggc cgcgttgatg
     5041 cacgacgtgg ggaaaatcgc cgtgcccgac agcatcctca acaagaatga gaacctcacg
     5101 gaggaggaat accgcaccct gatgcgcgtg ccgatgatcg gcgcggagat cctctctccc
     5161 atcaaacagc ttcgcggcgc cgttcccgtc atccggcatg ccaacgaacg ttgggacggg
     5221 aaagggtatc cggaccggct cagcggttcg aatattcccc aggggtctcg cgtcctggcg
     5281 gtcgccaaca cgttcgacgc catgaccacc gaccggccct atcgccaggg tctccccgac
     5341 gacgtggctt tgaaggagat ggcgtcttat gccggcgtgc agttcgaccc cgcctgcgtg
     5401 gaggcgttca tccgcgccta tcgcaagggg aagttcaagg gaatcacccg ctgacccgtc
     5461 cgggagggga tccgtggatc gttttatcgt cgttctcatc accgcttcct cgtccgccga
     5521 gtccgcgcgc ctttcgaagg ccctggtgaa ggaaagactg gccgcctgcg tcaacatcgt
     5581 gcccggcctc cggtccgtgt atcggtggaa agggcggatc gaaagttccg ccgaacggct
     5641 tttgatcgcc aaaacacggc gatcgaagct ccctcaattg atccgccgcg tccgccaatt
     5701 gcattcctat tccgttcccg agatcatcgc ccttcccatg ctcgccggcc acgcggatta
     5761 cctgcgttgg atcgccgcgt cgctcgttcc cgaaaaataa cggcccgttc ccggaaagaa
     5821 acgtgttgaa gaaccggttt ttcccgtggg tgatgacgct ttccgcgttt ctcttgtccg
     5881 cgacggcggt ttttcccatg gaaccgatcg ccgaaaagga ttttgtgtgc cgcggcgatg
     5941 gtgcggggag gatggtttgg gaggggaagg aatatcgcga ctgccccggc ggtcatttgc
     6001 cggccggcgg cgtccgtccg gagctgcttg ccctcctgaa cgcgctgcag gcgcgcgtcg
     6061 gggcggcgtt ggtgttgacg tccggttacc ggtgcgaggc gcacaaccgc tattcgtggg
     6121 cgtgcctcgc cagggacggc gatgccgccg gcgtcagccg gaggagtctc catcggtccg
     6181 gcgcggccgc ggatttttat gtggaggggg tccgggacat ttcaaaatac cggacgttcg
     6241 cggaagagtt gcggcggacg gccgcggctt cgccggggaa gacggtgtgg acgcgcgtct
     6301 atggggccga agaggtgcgc gaccccgaca accggcacga ctacccgtac atccatgtcc
     6361 atctgcttgg ggtaccgcgc cgaccgctgg agccggcctc ggattagggg aggagaaccc
     6421 gtcctttttt gacccgcagg gaggtgtcga gttcggcgct gagttgtttc agccggtgga
     6481 tgaatttccg cagcgccgct ttttttaggg gccgggtttg aaaccccgtt ttggtgtttt
     6541 ggacgagcac ggggagtatt tccgcccggg gggaatgacc ggtccggagt ttcagcttga
     6601 ggaccaaccc ttcgacggcg ttcgggttcc ttgtcccgaa gacgaaattc ccaaggctgt
     6661 aggcaatgat ctttttcttg taacgttcca gcccctgggc cacgtgcgga tggtggccga
     6721 tgaccgcgtc cgcccccgcg tcgatggccg tgtgagcgag ttttcgctgc aggtcggagg
     6781 ggaggtaggt gtattcctct ccccagtgga acgacacgat gacccaggtg cagtattttt
     6841 tcgcgtgggc gatgtcctcg cggacgagct tttcgtcggc aaaggccgtc ccgggggtcg
     6901 acggaccggc cgagaaggcg atggggaacg tgagcgagta ggccagaaaa gcgatgcgtt
     6961 tcccgcggac catcaccacg gcgggtttgc gggcttcttc gatatttttc ccggcgccgc
     7021 aggaggcgat cccctgatcg tagagatgcc ggcccgtttc cgccagcgcc tccggtccga
     7081 aatccaggat gtggttgttg gccaggctca tcacgcggaa gccggcttcc cagagcgcgg
     7141 cggcgtgggc cggttccatg cggaagatgt attccttgtc cgggtactcc tcgcccgtcg
     7201 tgccgatggg gttttccagg tttccgaaga cgaagtcgct ttttttcaga agggcgcgga
     7261 tcttgtcgaa cggatagtgc gggcctttcc gtttcagcac aggtttgaga tggtaggcca
     7321 tcatcacgtc tcccacgcag gcgatttccg cctgggaggc cgagagcgac ggcggggcga
     7381 tgtggaaaaa cgcggcggcc agaaccaccc gggggagtag ccgccggaag gtattttcga
     7441 aagaa
//
LOCUS       DAOTFD010000123         7353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7181_length_7353_cov_4.244039, whole genome shotgun sequence.
ACCESSION   DAOTFD010000123 DAOTFD010000000
VERSION     DAOTFD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_7353_cov_4.244039"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="P5079_07635"
     CDS             <1..531
                     /locus_tag="P5079_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY29895.1"
                     /translation="GRMGVFQILFEWISSGRNIYTIGDGNVLFQFIHVDDLLNAYMLL
                     LKKEQTGIFNVGADRFGTLREALERLVRYAGTSSRVKALPVHPTIAALRLLDFLRLSP
                     LAPWHYLTYHTPFFVDSRPLLQLGWKPVYSNDEMLRESYEWYLAHRRSREELSHLSPH
                     CRPVKERILWALKKMS"
     gene            1029..1100
                     /locus_tag="P5079_07640"
     tRNA            1029..1100
                     /locus_tag="P5079_07640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1061..1063,aa:Val,seq:tac)
     gene            1223..1300
                     /locus_tag="P5079_07645"
     tRNA            1223..1300
                     /locus_tag="P5079_07645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1258..1260,aa:Asp,seq:gtc)
     gene            1508..1795
                     /locus_tag="P5079_07650"
     CDS             1508..1795
                     /locus_tag="P5079_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HRY29896.1"
                     /translation="MPDLSLPWRHPLPGETLAGVVDDYFSHIGVITFTLRKPLQVGDQ
                     IHVRGHTTDLTQEVVSLQIEHDSVKEAAVGKGVGIKVDDKCRRGDFVYLVE"
     gene            1814..2458
                     /locus_tag="P5079_07655"
     CDS             1814..2458
                     /locus_tag="P5079_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRY29897.1"
                     /translation="MSLNKGSVVSIAKRLLPGFILGAALVVAVLSARQSSWTAPFQVP
                     SFRQQGPASAPVLLVEFSDFECANCAKARIAVHELLERQKGRVRLQFRHFPLKTHRWS
                     SMAALAADCAGVQGRFWEYHDLLFENQKEWAKADDARGFFIEYARRTGLDMERFRQDL
                     AGGRWMPLVQNDLEDGKSRGVNATPTFFIGARRLVGESQLRAYGDQFVEIEGPK"
     gene            2455..2949
                     /locus_tag="P5079_07660"
     CDS             2455..2949
                     /locus_tag="P5079_07660"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRY29898.1"
                     /translation="MNLRALTENRGFPWMAAAARVAAGGPLAFSGFMKLMSPPEELAL
                     TMEAYRILPFSLIPPFARLMPWGELFVGAYLVVGFFIRLSGTLAAGFYCMFLTALLSA
                     VVRGISLNECGCFGQGGPHLSGWGAITLDAFLLLFALAALLDRRHYLSLDRLHLRQKG
                     PLPE"
     gene            2946..4070
                     /locus_tag="P5079_07665"
     CDS             2946..4070
                     /locus_tag="P5079_07665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY29899.1"
                     /translation="MTKNIPLIDLHPTHDPLEKQMVAAFKSVYERNAFVLGAEVQSLE
                     KAFARLCGVKEGIGVSSGTAALTLTLRALGIGPGDEVLTTPFSFFSTASCIAYVGARP
                     VFADVEEDTLNLDPAKLEALRTDKTKAVLPVHLFGHPARMDAIRRFAQKHGLLIVEDA
                     CQAHGAKYLDRSVGGWGDAACFSFYPSKNLGGLGDGGLVVTDNAELAGRLRVLRNCGR
                     RDVPYEHSELGYVERLDNMQAAFLLVKLKFLGRWNAERKKLVRCYEEGLAGTPVHPLP
                     SLPEAAPVYHLFTVRTPRRDDLQKYLTGQGIGTGICYKTPLHLQKAFAYLKGRRGDFP
                     VAEKAAEEVLSLPLYAGLSPAAVRRVCAEIRRFFSRSSNG"
     gene            complement(4048..5256)
                     /locus_tag="P5079_07670"
     CDS             complement(4048..5256)
                     /locus_tag="P5079_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29900.1"
                     /translation="MSSSSDPQKIPTRLLVLLAAAGTALRIAYVVFTWGDIAHPDLSS
                     FTHPAGLMTHLFDTSPREPVFVWWLWPLLKSGLSSTPALRLVTLFWFLPTFFLFHAVA
                     RRYLGKTAVLVVLAAYAFLPAQIHSDSLGLRQTLEGTGLLLLFLTIARTDTFSRKALF
                     ITAGAATLLILTRINYLASVGLLLAAAAFRLKTPRPLLAVLPAIFLLLFHFQNNKSKW
                     GDPLFSVNLHSYWFSNLEYIGQPGFPATQDEWQKAPYKPGLTFREWAFQRHRFSEFVK
                     ETAMGLPRCLWVFYERIYFSYKLPLAVKWGFLVLYVAGFLLALFKKGWRFLPFGLVCL
                     ILPYSFISHVFWAGRFFVPFSPLVLLLAVHTGEEIVFLTKNKRSPIELFRRPLSKPAA
                     DDGLTRSKTG"
     gene            5327..6586
                     /locus_tag="P5079_07675"
     CDS             5327..6586
                     /locus_tag="P5079_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRY29901.1"
                     /translation="MCAAAAGIFLFSGCFRNPVTHRREAKLISEEAERRIGQESQSRL
                     IEEYGEFKSPVMREYVAALGNKLAAVSDRARLPFEFTILDTDMINAFAVPGGYIFVTR
                     GLLEELTAEAELAAVLGHEIAHVCAWHSVNMIEKQMGYGTLAALGAIVSGIQLGPEAM
                     VMVAQTADLFTSLYLLGYSRDYELEADRVGLRYAISAGYDPRSAITFFEKLRELEKQE
                     GEDKWEPYLRSHPPTEDRIKQADAYIGRMNVSDRDLTEGVEKYQDLKDYLPHLSPEEK
                     GRTEGQKFIQPAWKVSLEIPGGWVWEGRSRSALAVFREARGGGWGELRRRLIGPRSDA
                     TLFAKRVAGEKKWTFIQGRSVLYPSGYGYLGQYYGAGTLGGYFQLRVLFVTRGTEGYY
                     LVCAAPPEKFANYLIPFEQILRSFRLE"
     gene            6663..7058
                     /locus_tag="P5079_07680"
     CDS             6663..7058
                     /locus_tag="P5079_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29902.1"
                     /translation="MARRRILIIDDDDAFRTIVREALGKQGYEIREAANGREALAQVG
                     QFQPELVLLDVMMPDMDGVTLCRELRAVPTMKDVPVLMVSALGDNQTTNDALLFGATD
                     YVVKPVELAQLRAKIDKAFQESENRRRMR"
     gene            7187..7259
                     /locus_tag="P5079_07685"
     tRNA            7187..7259
                     /locus_tag="P5079_07685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7220..7222,aa:Phe,seq:gaa)
BASE COUNT         1481 a         1927 c         2393 g         1552 t
ORIGIN      
        1 ggaagaatgg gcgtctttca gatcctgttt gaatggattt catcgggacg gaacatttat
       61 accatcggag atgggaatgt actttttcag ttcatacatg tcgacgattt gctgaatgcc
      121 tacatgctat tgctgaagaa agaacaaacg gggattttta acgtgggggc cgatcgattt
      181 ggcaccttgc gggaagcgtt ggaacgcctc gttcggtatg ccgggacgtc ttctcgggtg
      241 aaagccctcc ctgtccaccc gaccatcgcc gcccttcgac tgttggactt tctgcggctg
      301 agcccattgg ccccctggca ttacctgacg tatcatacgc cgtttttcgt tgacagccgg
      361 cctttgctgc aattgggatg gaaacctgtc tactccaacg atgaaatgtt gcgtgaaagc
      421 tatgaatggt atctggccca tcggaggagt cgagaggagt tgtcgcattt gtcgccccat
      481 tgccggccgg tgaaagaaag aatcctttgg gcgttgaaga aaatgtcgtg aggccgcgtt
      541 tgtcgaagat ggcctttttt ctgaaaaaga atctcatccc tctggcggat tgagcgaaga
      601 ggggtttaac gacttcagcg caagggaatt tgaccgttca atgaaatctg ctacaatcat
      661 cgagcgcagg acgacttctc gcaattcaca gggagggcgt cgcctgactg ctgatggaaa
      721 cggcccgttt tttccggatg tgtggttttt tcggtctttg acaggttctt tgagtcggtc
      781 tcggccggac tcgcgctcgg agatttttaa ttccgcgagt tggggcgcat tggcaaaaga
      841 cgatgccaaa aggggagagg gggcaaggca cggggaccgg aaatttcgga tacgtttttc
      901 ttcctgtgat ggcggtttgc gcaatccttc ggaagacggc cggaaggggc gatttttcaa
      961 tcgacgtaaa cggcccgaga gggataagcc tgaattcgaa aatgcagttg tatcgttcag
     1021 cgatattcgg gcggttagct cagcggtaga gcattgccct tacaaggcag gggtcagagg
     1081 ttcgaatcct ctaccgccca gatttacagc agggctgagt tctgaggaat gggggccgac
     1141 ggcaggtcgg agccgattaa tttttttgtc cccacgcagc gattcgggga cctacggcgc
     1201 gcagaggcgc ggctgttccg taggggtcgt ggtgtagctt ggttaacacg tcgccctgtc
     1261 aaggcgaaga ccgcgggttc aaatcccgtc gaccccgcca cttttaaact cgaaatggcg
     1321 cagaaacaac cgggaaagga ggatgtccat gccaaaacgc aaggccagta aaaagaaaac
     1381 cgtcgcgaag aagaaaaaat ccattcctcg agggaagtca gccaagaaat cttccgctaa
     1441 gaaagcacct cccaaaacaa aaaaaacgaa aaaggcttct gcaaaacccg tttcctcgag
     1501 ggggggattg ccggacctca gccttccgtg gcgtcatccg ttgccgggcg agacgttggc
     1561 gggcgtcgtt gatgactatt tttcccacat cggagtgatc acctttactc tccgaaaacc
     1621 cctgcaggtg ggggaccaaa tccacgtgcg cggacacacg acggacttga cccaggaagt
     1681 cgtctccttg cagatcgagc acgactcggt caaggaggcg gcggtcggta aaggggtcgg
     1741 catcaaagtg gacgataaat gccgtcgcgg ggatttcgtt tacctcgtcg aataaaccca
     1801 ccctcccttc tccttgtcgc tgaataaagg atcggtcgtt tcaatcgcga agcggcttct
     1861 gccgggtttt atcctcggcg cggcgctggt tgtcgccgtt ctgtcggccc ggcagagttc
     1921 ctggacggcg ccgttccagg ttccgtcgtt ccggcagcaa gggcccgcct cggccccggt
     1981 gttgttggtg gaattctccg atttcgaatg cgccaactgc gccaaggcgc ggatcgccgt
     2041 gcatgagttg ctggaacggc agaagggccg tgtgcggctg cagttccgcc attttccgct
     2101 caaaacgcac cggtggtcgt ccatggcggc cttggccgcc gattgcgccg gtgttcaggg
     2161 gcggttctgg gaatatcacg atcttttgtt cgagaaccaa aaggaatggg ccaaggccga
     2221 cgacgcccgg ggatttttca tcgaatacgc ccgccggacg gggctggata tggaaaggtt
     2281 ccggcaggac ctcgcgggcg gccgttggat gccgctcgtg cagaacgatt tggaggacgg
     2341 gaagtcccgc ggcgtcaacg ccaccccgac ttttttcatc ggcgcccggc gtttggtggg
     2401 ggaatcccag cttcgggcct acggcgacca gttcgttgaa atcgaggggc cgaaatgaat
     2461 cttcgggcgt tgaccgagaa ccgggggttc ccctggatgg cggcggcggc tcgtgtggcg
     2521 gccggcggcc ccctggcgtt ttccgggttt atgaaactta tgtcgcctcc ggaagaactg
     2581 gcgttgacca tggaagcgta tcgtatcctg ccgttttccc tcatcccgcc gttcgcccgt
     2641 ctcatgccgt ggggggaact tttcgtcggc gcctatctcg ttgtgggatt ctttatccgt
     2701 ttgagcggta ccctggcggc gggtttttat tgcatgttcc tgacggccct cctttccgcg
     2761 gtggtgcgag gcatttcctt gaacgaatgc ggctgtttcg gccagggggg gcctcatttg
     2821 agcggatggg gggcgatcac gctggatgcg ttccttttgc tcttcgcgct cgcggcgttg
     2881 ctggatcgcc gacactatct gagcttggat cgactccatc ttcgacagaa aggccccctt
     2941 cccgaatgac aaaaaatatc cccttgatcg atctccaccc gacgcacgat ccgctggaaa
     3001 aacagatggt ggcggccttt aagtccgtct atgaaaggaa cgcgttcgtc ctgggggcgg
     3061 aagtccaatc gctcgaaaag gcgttcgcgc ggctttgcgg ggtgaaagaa gggatcgggg
     3121 tctcctccgg gaccgcggct ttgacgttga ctcttcgggc gctgggcatc ggcccggggg
     3181 acgaggtcct caccacgccc ttctcctttt tttccacggc ctcctgcatc gcctacgtgg
     3241 gggcgcggcc ggttttcgcc gacgtggagg aagacacgct gaatctggat ccggcgaaat
     3301 tggaagctct ccggacggac aagaccaagg ccgttttgcc ggtgcatctc ttcgggcatc
     3361 ccgcgcgcat ggacgccatt cggcggtttg cccaaaagca tgggctcctc atcgtggaag
     3421 acgcctgtca ggcgcacggg gccaagtatt tggaccgttc cgtggggggc tggggggatg
     3481 cggcctgttt cagcttctat ccgtcgaaaa atctgggcgg tctgggggac ggcggcctgg
     3541 tcgtcacgga caacgcggaa ctggcgggcc ggctccgggt gcttcgcaac tgtggccgcc
     3601 gcgatgtgcc gtacgagcat tcggaactgg gctatgtgga gcgtttggac aacatgcaag
     3661 ccgcgttcct attggtgaag ctgaagtttc tcggccgatg gaacgccgaa cgcaaaaaac
     3721 tggtccgttg ttatgaggag gggctggccg ggacgccggt gcaccctctg ccgtctttgc
     3781 cggaggcggc gccggtctac catcttttca ccgtgcgcac gccgcgccga gacgaccttc
     3841 agaaatattt gacgggccag gggatcggca cggggatctg ttataaaacg ccgctgcatc
     3901 tgcagaaggc gttcgcgtat ctgaagggcc ggagggggga tttcccggtg gcggagaagg
     3961 ccgcggagga agttttgtcc ctgccgctct atgccggtct ttccccggcg gcggtgcgcc
     4021 gggtgtgtgc cgagatccgg cggtttttca gccggtcttc gaacgggtga ggccgtcgtc
     4081 ggccgccggt tttgagagag gccgacggaa caattcaatc ggactccgtt tgttttttgt
     4141 gagaaaaacg atttcctcgc cggtgtggac cgccagcagc aacacgagcg gagagaaagg
     4201 gacgaagaac cttcccgccc aaaagacgtg actgatgaag ctgtaaggca aaatcagaca
     4261 gaccagcccg aacggcaaga aacgccatcc ctttttaaac aaagccaata aaaatccggc
     4321 gacatagaga accaagaatc cccatttgac ggccagcgga agtttgtagg aaaaatagat
     4381 ccgttcgtag aagacccaga ggcagcgggg aaggcccatc gcggtttctt tgacaaattc
     4441 agaaaaacgg tggcgctgaa aggcccattc cctgaacgtg aggcccggtt tgtagggcgc
     4501 tttctgccat tcgtcctgcg tggctgggaa gcccggctgg ccgatatact ccagattcga
     4561 gaaccagtaa ctgtgcaggt tgacggaaaa aagagggtct ccccatttgc ttttgttgtt
     4621 ctgaaagtga aagaggagaa ggaaaatcgc ggggaggacg gcgaggagcg ggcgcggcgt
     4681 ttttagtcga aaggccgccg cggccagcag gagtcccacg gacgcgagat agttgatgcg
     4741 cgtcaggatg agaagggtgg ccgccccggc cgttatgaaa agagcttttc gggaaaacgt
     4801 gtccgtgcgg gcgatggtca gaaagagcaa aaggagtccc gtcccttcca gcgtctggcg
     4861 cagccccagg gagtcggaat ggatctgggc gggaaggaac gcgtaggcgg ccaggaccac
     4921 caggacggcc gtttttccaa gatagcgtcg cgccaccgcg tggaacagga agaacgtcgg
     4981 aaggaaccag aacagcgtga ccagccggag cgccggcgtg gacgacaggc cggatttcaa
     5041 caggggccag agccaccaca cgaacaccgg ttcccgcggg gaggtgtcga acaggtgggt
     5101 catgagaccg gccggatgcg tgaaactgga aaggtccgga tgggcgatgt ctccccacgt
     5161 gaagaccacg taggcgatac gcaacgccgt gccggcggcg gccagaagca ccaggagccg
     5221 ggtcgggatt ttttgaggat ccgaggaaga tgacatgggt gcccagaggt taacattttg
     5281 gaatggcttt ctccacggtt ttcgccgtgg ggatcggcgg tgtttcgtgt gcgccgcggc
     5341 ggccgggatt ttcctgtttt cggggtgttt ccggaacccc gtgacgcatc ggcgggaagc
     5401 caaactcatt tccgaagagg cggaacggcg catcgggcag gagtcccaga gccgactgat
     5461 cgaggaatac ggggagttca agagcccggt gatgcgggag tacgtggctg ctttggggaa
     5521 caagctggcc gccgtgagcg accgcgcccg gctgccgttc gaattcacga tactggacac
     5581 ggacatgatc aacgcgtttg ccgtgcccgg cggctatatt ttcgtgacgc gcgggttgtt
     5641 ggaagagctg acggccgagg cggaactggc ggcggtgttg ggacacgaga tcgcccacgt
     5701 ctgcgcgtgg cattccgtga acatgatcga gaagcagatg ggctatggga cgctggcggc
     5761 gttgggggcc atcgtgtccg gcatccagct cgggcccgag gccatggtga tggtggccca
     5821 gacggcggat ctattcacca gtctctatct cctcggctac agccgggatt atgagttgga
     5881 ggcggaccgg gtggggttgc gatacgcgat atcggccggc tacgatccgc ggtcggcgat
     5941 cacgttcttc gagaagttgc gggagttgga gaagcaggag ggggaagaca agtgggaacc
     6001 ctatctgcgt tcacatccgc cgacggaaga ccgcatcaaa caggccgatg cctacatcgg
     6061 ccggatgaac gtatccgacc gggacctcac ggagggcgtg gaaaagtatc aggacttgaa
     6121 ggactatttg ccgcatttgt cgccggagga aaaaggccgc acggaagggc agaaattcat
     6181 tcagcccgcc tggaaggtgt cgttggagat cccgggcggt tgggtgtggg aagggcgcag
     6241 ccggagcgcg ctggcggtgt ttcgcgaagc gcgcggaggg gggtgggggg aactgcggcg
     6301 ccgcctcatc ggtccacggt ccgacgcgac gctcttcgcc aaacgcgtcg cgggggaaaa
     6361 gaagtggacg ttcattcagg ggcgatcggt gctgtatcct tcgggctacg gttatctcgg
     6421 ccagtattac ggtgcgggga ccttgggcgg ttatttccaa ttgcgcgtgt tgttcgtgac
     6481 ccgcgggacc gaggggtact atctggtttg cgccgctccg ccggaaaaat tcgccaatta
     6541 tttgatcccg tttgagcaga ttctgcgttc tttccgtctg gaatgagccg ccttgcgtgg
     6601 tttggggcca cttgctatac tgctttggct tgtgacgggg aaaacttccg gatttgaaaa
     6661 ttatggctcg ccgccgcata ctcattattg acgatgacga cgcttttcgg acgattgtcc
     6721 gcgaggcgct tggaaaacag gggtatgaga tccgcgaggc ggccaacggg agggaagccc
     6781 tcgcccaggt gggacagttt cagcccgaac tggtcctgct ggacgtgatg atgcccgata
     6841 tggacggggt caccctttgc cgggaactcc gcgccgtgcc gacgatgaag gacgtgccgg
     6901 tcctcatggt cagcgccctg ggggataacc agacgaccaa cgatgcgttg ttgttcgggg
     6961 ccacggatta cgtggtcaag cccgtggaac tggcgcagtt gagggcaaag atagacaaag
     7021 ctttccagga atcggagaac cgccggcgaa tgcgttgagt tttccgctga agtgccgccc
     7081 ccttgtccaa gacaacaagg acaggcatta tctcaaatag ccctttcgca aagctttcag
     7141 gacggtggtc atgccagagg catgaaaacc accaccgaat ttaagtgccg aggtagctca
     7201 gttggtagag caacggtctg aaaaaccgtg tgtcggcggt tcaattccgt ccctcggcac
     7261 ttttgttttt ttaggggccc tatgttctgg aaattgctcg ccgatgtcgt gaccgtcctc
     7321 catcttttgt gggtcgtcgg ggtgcttttg ggg
//
LOCUS       DAOTFD010000124         7305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7237_length_7305_cov_4.183724, whole genome shotgun sequence.
ACCESSION   DAOTFD010000124 DAOTFD010000000
VERSION     DAOTFD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7237_length_7305_cov_4.183724"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3265
                     /locus_tag="P5079_07690"
     CDS             <1..3265
                     /locus_tag="P5079_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29903.1"
                     /translation="EAEIAKRKAEDEAKAKAARPAEVEAKAPTAELSPETEMEIQDYL
                     NDRVEREKITVRLLDLTENYIRGQIAAERDEAQRAKLETFYAGALKMVAQDRQYLKDF
                     NNPEWLERQIQRDQSKLATSKKMRKATREAVERRVALFQKQLGLLRGERPGPEVEPEV
                     ARPPEGEMDVPTKADRKRAGKKTAAAGGQRGAVSIEMLLVLSGAGALVLLAVPVAKIV
                     GIVLVAKVGVAIVVGAALAAGVYYAVKHASSWWSAARDYAGKALGRVSKAKAVVSDAW
                     RSGLKLFRAKTTTGPRKYSKQDLQQRMEQRMAALQKEGADVSALAKEATPSAKKTGEA
                     VTFRGVMGTAWAAVYGRKSWLMYQLRGYRIYEFSELARALDDYVLQNREPLAKLYPYD
                     ENQLSAEVPVIVNIHWSASGPRAEVLPINGVVALPGEKRVHPEYLVRQGEGHNATALD
                     GTVAVDAKLFGRRAIPGHFHNHGDLTPTVQDVVTGKHIGVVLVKNAQGIVQAGLYQWV
                     AEKGKPSVVRTHATLQKEPVVKDFGVFFAEGMGPRSEKLYLDDMDADFMQVVLKAMYE
                     NEDLPSILERLGISSEKEYKRFSVMVRGAAGAGVAGTEGTLAVRDIPNFVRLSVSMAF
                     YLEGKPAVPAYILQARTDEILRKLNLDWISSQEAMERLARLRSDSAGFQAAIYEGALR
                     TMPQDLTRPAFLAAVLQRPETFAQLSSSQQQQVLEYLARALLIPTVDAALGASNIQVR
                     ISSVPMGARSRIRVNEAVSMENGEEKVFRTVDVLLQTSTVSGESRLFDYLTSLAHELS
                     HLALWQGSRYPAGEGFEPEDVEAITAVYMREVLRGTVLAGLSGNDEKEFLDHLIKHSA
                     GDEGVLQLWNLRRLAGALTEQYPDKSQRRAFLHWLFRTATVARLNLRQIAADLVADLP
                     SRRWALPRVMATNGDSFVRGGVVNEGLLFLMNRCFEEERALNENDRQLRLVILGNVMS
                     PADLSVLKLALEKYPALNEAWNNGRGGRLLLLEKDAYGYTTDKNEIDMGILFAVMPKI
                     LNLDQRVLQMGLSIFTDREDLWVNNFRQIVVHLISGTTLAKGLLDAIAHMQIFDIQA"
     gene            3397..4716
                     /locus_tag="P5079_07695"
     CDS             3397..4716
                     /locus_tag="P5079_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY29904.1"
                     /translation="MKSKALFTFWVVLGACPLLGGDTPFVALETDALVEKWESGASDG
                     DRRAYVTALRTLFERAEAAGDSGDMFLLASRLGELDPAQARWTSLREKALGELAFPSE
                     KLTRSVGASYEARLRGAERRMRTMCSAGQRVSLLDRWRMHRYRGLQKRLWREERLRVM
                     RAARAAAARTPVDRDQCDRRLAKTERRIEKLTRRSNRYRQGLEERGLGETAWRIDSPD
                     KWAAAVELQSARYAVGRFLRWEKRYWADYYGAPGKLLSEIPLSADPISIPIPKAKKER
                     TVALRPASTGFERPRFVSGPSARLTPPAAGPSPAPNPVLPDDYIVQPGDTLYSIARAL
                     YGDGSAWRNIYERNRDAILRGLLGTGLRLRLGESPVREESSGRSAGPTAASSGHVRLY
                     RAQSGETLPQLAGRLYGDPGRWGDIYAANQERLSRGLLEKGMLLVIP"
     gene            4727..6436
                     /locus_tag="P5079_07700"
     CDS             4727..6436
                     /locus_tag="P5079_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29905.1"
                     /translation="MDNTRGPIDKKPTLTSWAWQTPEDIFADTQRILQEGDMLLSLQR
                     KGMRMEEEIAHLRRRLSELQTQARSQEEVRDEERKRREEEVGALSQKYERIVLDYRQE
                     LETLRKVLEHDKGVFEKQFEGARGAWRQEQAVELEKLRQAYERELEEHRAGMGRLEAE
                     RRREKKTAEDVLSAAQNESRRLAETVAAKEKKIAELQAGLEEFRKKVVRESVPGPEEK
                     GRPSAVPVPRTEPSVGRGDLGADFKELWQEERAQWKTIFEKEKELQSKWLDNVLQDHQ
                     AVLEERRHFAEALKIFSQGVAEWGRREADAALRPKKESGSWRPTPSAGFGAMFFALCL
                     GVAGTMLLRRSDVPAGVYSVPTIHAGGVVSDGKKVWFSDWSDGRFFVAPSIDPAAALS
                     AGRASEGYHPVSLRLVGGQLWSLDAWGKTLHRHNPKPPFEIVESKPFGPVAAPSDFAW
                     DGKSFWILDKDARQLLRFSPEELTRPQNVVSLPADWQLELLDFWEGDFWSYDVRSLRV
                     KRFRVQPELRLVDEFQLPGAPQWLSGLNVSDGFLWVLSEKSQTIQRWSLKKLALRKLL
                     GAS"
     gene            6511..>7305
                     /locus_tag="P5079_07705"
     CDS             6511..>7305
                     /locus_tag="P5079_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRY29906.1"
                     /translation="MAAVCLCCFAAASAFGWGGWGHERINGNCVRGLPSPLREYFASK
                     KAVLRKHASDPDAWKYDDADEAHYHYLDIDALEPYPFNDFPRDLEEAYETYGEDRVKT
                     AGELPWRIEAYVQTLTEDFSDHRWKDAVFTASVLGHYVADLHQPLHVTENHDGQLTGN
                     EGIHSHFESGLVERYGDGMVLQVSSAVYVRDVFDFCMKAVRSGYPLVEEIMEADTSAG
                     QRAGGNEERYYAELHRAVGKAAERRMGEASSGLASLIYTAWVNAKKP"
BASE COUNT         1425 a         2040 c         2455 g         1385 t
ORIGIN      
        1 cgaggcggag attgcgaagc ggaaggcgga ggacgaggcc aaggcgaagg cggcgcgccc
       61 ggctgaagtc gaagcgaagg cgccgacggc ggagttgtcg ccggaaacgg agatggaaat
      121 tcaggattat ctgaacgacc gcgtggagag agaaaaaatc accgtccggc ttttggattt
      181 gacggagaat tatatccgcg gacagatcgc cgcggagagg gatgaggcgc aacgggcgaa
      241 gttggaaact ttttacgccg gggcgttgaa aatggtggcg caggaccggc agtatttgaa
      301 ggatttcaat aacccggagt ggctggaacg gcagatccaa cgggaccagt caaaactggc
      361 caccagcaaa aagatgcgga aggcgacgcg cgaggcggtc gagaggcgtg tggccctctt
      421 tcagaaacag ttgggcctgc tccgcggcga acggccgggg cccgaggtgg agccggaagt
      481 ggcccggccg ccggaagggg agatggatgt tccgacgaag gcggacagaa agcgggcggg
      541 gaaaaagacg gcggccgccg gcggacaacg cggcgctgtt tccattgaaa tgctcctggt
      601 tttgtccgga gcgggggcgc tggttttgct cgcggtgccc gtcgccaaaa tcgtggggat
      661 agtccttgtg gcgaaggtcg gcgtggcgat tgtcgtcggc gcggccttgg ccgccggtgt
      721 ctattacgcg gtcaagcacg cgtcttcgtg gtggtccgcc gcgcgagatt acgcggggaa
      781 ggccctcggc cgcgtttcga aggcaaaggc cgtcgtttcc gatgcttggc ggtccggtct
      841 gaaacttttc cgcgccaaga cgacgacggg ccccagaaaa tacagcaagc aggacctgca
      901 gcagcgtatg gaacagagga tggcggcgct gcagaaagag ggggccgatg tctcggcgtt
      961 ggcaaaagag gcgacgccca gcgcgaaaaa gacaggagag gccgtcacct tccgaggggt
     1021 catggggacg gcttgggcgg cggtctacgg acggaaaagc tggcttatgt atcaattgcg
     1081 gggataccgt atctatgagt tctcggaact cgcccgggcg ttggacgatt acgttttgca
     1141 gaacagggag ccattggcca agttgtatcc ctacgatgaa aatcagttga gcgcggaagt
     1201 gccggtcatc gtcaatatcc actggagcgc ctccggcccg cgggcggaag tgcttcccat
     1261 caacggcgtt gtggccctcc cgggagagaa gcgtgttcac cccgaatacc tggtcaggca
     1321 gggcgaggga cataacgcca ccgctctgga cgggaccgtg gccgtcgacg cgaaactgtt
     1381 cggtcggcgc gccatcccgg gccacttcca taatcacggc gatttgacgc cgaccgtgca
     1441 ggacgtggtc accggaaaac acatcggcgt ggtgctggtc aaaaatgcgc agggcatcgt
     1501 ccaggcgggg ctgtatcagt gggttgccga aaaggggaaa ccgtccgtcg tccgcacgca
     1561 cgccaccctg cagaaggagc ccgtggtgaa ggatttcggc gtgttcttcg ccgaggggat
     1621 ggggccgcgc agtgagaaac tctatttgga cgatatggac gcggatttca tgcaggtcgt
     1681 tttgaaggcc atgtatgaga acgaggattt accctccata ttggaacgtc tcggcatttc
     1741 ttcggagaag gaatataaac ggttcagcgt catggttcgc ggggcggccg gcgccggggt
     1801 ggccgggacg gaaggaactt tggcggttcg ggacatcccc aatttcgtca ggttgtccgt
     1861 ttccatggcg ttctatctcg aaggcaaacc ggccgtcccg gcgtatatcc tccaggcccg
     1921 gaccgacgag atactccgga aactgaacct ggattggatc tccagccagg aagccatgga
     1981 acgtctggct cgcctgcgtt ccgactcggc cggctttcag gcggccattt atgagggggc
     2041 cctccggacg atgccgcagg acctgacccg gccggcgttc cttgccgccg ttctgcagcg
     2101 cccggagaca tttgcccaat tgtcctcgtc gcagcagcaa caggttttgg aatacctggc
     2161 ccgcgcgctt ctcattccca ccgtggatgc cgctctcggg gcgtccaaca tccaggtccg
     2221 gatctcctcc gtgccgatgg gggcccgctc ccgcatccgg gtgaacgaag cggtgtcgat
     2281 ggagaatgga gaagaaaagg ttttccgcac cgtcgacgtc cttctgcaga cctccacggt
     2341 ttccggggaa tcgcggttgt tcgactatct cacctccctc gcgcacgagt tgtcccactt
     2401 ggctctctgg caggggagtc gttatccggc cggggaaggt tttgagccgg aggacgtgga
     2461 ggccatcacc gccgtctata tgagggaagt cctccggggc acggttctgg ccggactgag
     2521 cggcaacgac gagaaggaat tcctcgatca tctcatcaaa cattccgctg gcgacgaggg
     2581 ggtcctccag ttatggaacc tccgccggtt ggcgggggcg ttgacggagc aatatcccga
     2641 caaatctcag cgccgggcgt tcctgcattg gctcttcagg acggccacgg tggcgcggct
     2701 gaatctgcgg cagatcgccg ccgatctggt ggccgacctt ccgtcgcgtc ggtgggccct
     2761 cccgcgcgtg atggccacga acggcgattc gttcgtgcgc ggcggcgtgg tcaacgaggg
     2821 actcctgttt ctgatgaacc gttgcttcga ggaggaacga gccctcaatg agaatgaccg
     2881 gcaactgcgt ctcgtcatcc tcggcaacgt gatgtctccc gcggacctgt ccgtgctgaa
     2941 actggcgttg gaaaaatacc cggcccttaa cgaagcgtgg aacaacggcc gaggaggacg
     3001 actcctgctc ctggaaaaag acgcctacgg atacaccacc gacaagaacg agatcgatat
     3061 gggcatcctc ttcgccgtca tgccgaaaat cctcaatctg gatcaacgcg tgctgcagat
     3121 gggtctcagc atcttcaccg accgtgaaga cctctgggtt aataatttcc gacagatcgt
     3181 cgtccatctc atctccggaa cgacgttggc caaagggctc ctggacgcca tcgcccacat
     3241 gcagattttc gacatccaag cctgattccc ttccttttca ttccttaaac ccgccgtttt
     3301 ccctcttttt ccttggatgg aagtttgttc ttcccgcccc ttgaccggaa cggaagccgg
     3361 ggttacactt gataaaagta gtaaccatct ctaaaaatga aatccaaagc gcttttcacg
     3421 ttctgggtcg tgctgggggc gtgccccctt ctcggggggg ataccccttt cgtcgctttg
     3481 gagacggacg ctttggtgga gaaatgggag tcgggggcga gtgacggtga tcgtcgggcc
     3541 tatgtcacgg ccttgcggac gctttttgag cgggcggagg cggcggggga cagcggcgat
     3601 atgtttttgc tggcttcccg gctgggcgaa ctcgatccgg cgcaggcgcg ttggacatcc
     3661 ctgagagaaa aggctcttgg agaattggct tttccgtctg aaaagttgac gaggagcgtg
     3721 ggggcttcct acgaggcgcg cctgaggggg gccgagcggc ggatgcggac gatgtgttcg
     3781 gccggccagc gcgtgtcgct gctggatcgc tggcggatgc accgttatcg gggtctccaa
     3841 aaacggcttt ggcgggaaga acgcctccgt gtcatgcggg ccgcgcgcgc cgcggccgcc
     3901 cggactcccg tcgaccggga ccaatgtgac cgtcggttgg cgaaaacgga acgccgtatc
     3961 gaaaaattga cgcgccggtc gaaccgctat cggcagggct tggaagagag gggactgggg
     4021 gagactgcct ggagaataga ttccccggac aaatgggccg ccgccgtcga actgcagagc
     4081 gcccgctatg cggtcggccg gtttctccgg tgggaaaaga gatattgggc cgattattac
     4141 ggggcgccgg ggaaactttt gtcggaaatt ccgttgtcgg cggacccgat ttcgatcccc
     4201 atccccaaag cgaagaagga gcgaaccgtt gcgcttcgcc ccgcgtcgac cggttttgaa
     4261 cggccgagat tcgtctcggg accgtccgcc cggttgacgc cgccggccgc ggggccttct
     4321 ccggcgccga acccggttct gccggacgat tacatcgttc agccgggaga caccctttac
     4381 agcatcgcgc gggcgttgta tggagacggt tccgcttggc ggaacatcta cgagaggaac
     4441 agggacgcga tcttgcgcgg tctgttgggg acggggctcc gtctccgcct gggcgaatcg
     4501 cccgtccggg aggagtcgtc gggaagatcg gccggcccga cggcggcgtc ttccggccat
     4561 gtccggcttt accgcgcgca atccggcgag accttgcccc aactggcggg ccgcctttac
     4621 ggcgatccgg gccgctgggg agacatctat gcggccaatc aggagcggct ctcgcgcggt
     4681 cttttggaaa aagggatgct tttggtgatc ccgtagaggg cggagaatgg acaacacacg
     4741 cggtcccatc gataaaaaac cgacgctgac gtcctgggcg tggcagacgc cggaggatat
     4801 tttcgctgat acgcagagaa ttctgcagga gggggacatg ctcctcagcc tccagcgtaa
     4861 aggcatgcgc atggaggagg agatcgccca tctccgccgg cggttgagcg aactccagac
     4921 gcaggcccgg tcgcaggaag aggtccgcga cgaggaacga aaacgccggg aagaagaggt
     4981 gggggctctc agtcagaaat acgaacgcat cgtgctcgat tatcgccagg agctggaaac
     5041 gctccggaaa gtgttggaac acgacaaggg ggtttttgag aaacagttcg aaggggcccg
     5101 cggggcttgg cgccaggagc aggcggtgga actggagaag cttcgtcagg catacgagcg
     5161 ggaactggag gagcaccggg cggggatggg acggttggag gcggagcgcc gccgcgagaa
     5221 gaaaacagcg gaggacgtcc tctcggccgc acagaacgag agccgccgtc ttgcggaaac
     5281 ggtggccgcc aaagaaaaga agatcgccga acttcaggcg ggactggagg aattccgcaa
     5341 aaaggtcgtt cgggagtcgg ttcctgggcc ggaggaaaaa ggccggccgt cggccgttcc
     5401 tgtcccaaga accgaaccct cggtcggacg gggggatctc ggcgcggatt tcaaggaact
     5461 ttggcaggaa gaacgcgctc agtggaagac gattttcgaa aaagaaaagg agctgcagtc
     5521 caagtggctg gacaatgttt tacaggacca tcaggccgtc ttggaggaac gacggcattt
     5581 cgcggaggcc ctgaaaattt tttctcaggg agtggcggag tgggggcgcc gcgaggcgga
     5641 cgccgctttg cggccgaaga aggagagtgg gtcctggcgt cccacgcctt cggcgggatt
     5701 cggggcgatg ttttttgcgc tctgtctggg agtcgccggg acgatgctcc tccggcggtc
     5761 ggacgtgccg gccggcgtct actccgtgcc gacgatccat gccggcggcg tcgtttccga
     5821 cggaaaaaag gtctggttca gcgactggtc cgacggccgg ttcttcgtcg ctccctcgat
     5881 cgatccggcc gcggcgcttt ccgccggccg ggcctcggag ggatatcatc ctgtttcttt
     5941 gcgtttggtc ggcgggcaat tgtggtcgct ggacgcctgg gggaaaacgc tccaccgcca
     6001 taacccgaaa cccccgtttg agatcgtgga atcgaaaccg ttcggcccgg tcgccgcgcc
     6061 cagcgatttc gcatgggacg ggaaaagttt ttggatactg gacaaggacg cgcgccagct
     6121 tctgcggttt tctccggagg agttgacccg gccgcagaac gtggtgtcct tgccggccga
     6181 ctggcagctg gaactcctgg acttttggga aggggatttc tggtcctacg acgttcgttc
     6241 tctgcgggtg aaacggttcc gggtccaacc cgaactccgg ctcgtcgacg aattccaact
     6301 tcccggggcg ccccagtggc tctccggcct gaacgtttcg gacggctttc tgtgggtgct
     6361 cagtgaaaaa tcccagacga tacagcgttg gtccctgaaa aaactggcgt tgcgtaaact
     6421 cctcggcgcg tcttgaccct tgtcgccgcc gtccctcccc cggcccggat gacctctccc
     6481 gaacatcgtt tttcgttttt tcgaaaagcc gtggcggccg tctgtttgtg ctgtttcgcg
     6541 gcagcttccg cgttcgggtg gggagggtgg gggcatgaac gcatcaacgg caactgcgtg
     6601 agagggctcc catcgccttt gcgggaatac ttcgcctcga agaaagccgt tctgcggaag
     6661 cacgcctcgg acccggacgc ctggaaatac gacgacgccg acgaggccca ttatcactac
     6721 ctcgacatcg atgccctgga accctacccc ttcaacgatt ttccgcgaga cctggaagag
     6781 gcctacgaga cctacggcga ggaccgcgtg aaaacggccg gggagctccc ttggcgcatc
     6841 gaggcttacg tccagacgct cacggaagat ttttccgacc accgctggaa ggacgctgtt
     6901 ttcaccgcgt ccgtcctggg gcattacgtg gccgatttgc atcagcctct gcatgtcacc
     6961 gaaaaccacg atggccaact gacggggaac gaggggatcc attcgcattt cgagagcggc
     7021 ctggtcgagc gttacggcga cggaatggtg ttacaggtgt cttccgccgt gtatgtgagg
     7081 gatgtttttg atttctgcat gaaggcggtg cggtccggtt atccgttggt ggaggagatc
     7141 atggaggccg atacgtcggc ggggcagcga gcggggggga acgaagaaag atattacgcg
     7201 gaacttcacc gggccgtggg taaagcggcg gagcggagaa tgggagaggc ttcgtccggc
     7261 ctcgcttcgc tgatctacac ggcctgggtc aacgccaaaa aaccg
//
LOCUS       DAOTFD010000125         7278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7281_length_7278_cov_4.343486, whole genome shotgun sequence.
ACCESSION   DAOTFD010000125 DAOTFD010000000
VERSION     DAOTFD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7281_length_7278_cov_4.343486"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /gene="rodA"
                     /locus_tag="P5079_07710"
     CDS             <1..838
                     /gene="rodA"
                     /locus_tag="P5079_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRY29907.1"
                     /translation="APMTLVLLYSAGAPGGVLLSVAVFGFLALGIPLASTYFKLVGDR
                     SQDSWLWGGMRSVFLERRAFFLFWSGVCLSLAGGWWFLRKWRVFIPSLTLFCSLAVVV
                     SGVAGSFAVKKALKDYQRKRLIAFVDPSVDPLSAGYNILQSEIAIGSGRFFGKGFLGG
                     SQTQLGFLPEKHTDFIFSLLAEEWGFIGALIILLLYFFVVWRSFEIALAARERFGRYL
                     AVGLGTIFAFTGIVNIGMVMGLMPVTGLPLPFVSYGGSSLVGAYLAVGLLLSIHLRRY
                     VL"
     gene            898..2325
                     /locus_tag="P5079_07715"
     CDS             898..2325
                     /locus_tag="P5079_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRY29908.1"
                     /translation="MKKEIIVNHTPEEIRVAVMEDGRLVEYLIERPALGMAKLVGNIY
                     KGRIENVLPGISSAFVNVGYEKNAYLYVTDVVSDAKERHIEKMLQRGDPIMVQVAKEA
                     IGTKGMKVTMDISLPGRYLIYMPKSEHLGVSKNIEDAAERDRLRRAIEKTNPIGGVIV
                     RTEAEGAEERELAHEMRYLMRLWENIQKKYESTAPGALIHRELGLTFQVARDLLTDNA
                     HIFLVDSQEEFKDLRGFVEMLAPELADRVQHYTGRTPIFQAFGVEKELSQLRHQRINL
                     PSGGYIIIQEAESLCAIDVNTGKFIGHKSQEETVTATNLEAAAEVARQLRLRNIGGII
                     VIDFIDMRRRRNQHKVVEALQHATRNDRAKIKILPITRLGLVEMTRERRRESLLSLMT
                     EPCRECDGSGRVLSRESLYLKIQSEIEDLTKGRTDGTLKVSVPPVVGDFLRERQERLE
                     KNLQRSIEVQIDPTLPWEDYRILIE"
     gene            2804..>7278
                     /locus_tag="P5079_07720"
     CDS             2804..>7278
                     /locus_tag="P5079_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29909.1"
                     /translation="MLDRLIRDAEFGRFISGEESSEEVQRRRRAVSRLLSVSGHRYKI
                     HADGRKVWFKGGRVSRIENEPILQESGQVSRRDTTEMLYDEHGNLTSYRTETRNADGE
                     RLCVQWAGRYGPSPGGKYQLQGYIETRWDALGNASRLERSQVRWDPAGRAAAYRETET
                     DPWGRQTVREMSGIRYDSNGRPTSYRERIIGSDGGTQERWRVETRYDRLDGREEAVSF
                     YREIRGTADGGGDEVEWSAEKYDRLGRVRAWTEKTTDSSGGVTLCRRSDIEFDRNGNV
                     TGFEENENGPAGSRRRIWSDGVYDRWGRVVSYREIIVGGDQNVHRRWGGAEYNAKNEL
                     LSFAEELTDARGRTTQSRWKALEYQTGFVRDYQETTRVDDGGEVSHRWRGRHDAGGRV
                     TGFVDERIDPEGNREVSERNGITYDRAGRLAGFHEARTDNFGNKTIRTWTAQRYDAYD
                     RVLDYEEMETGAFGEERKRVWKNAQYDAQGRLASYEEDVRVDDRWIQRRWRADAYDSF
                     GQVTVFHEETSDPLCDETFRYWQGRYDREGRLVFSREENVEGTGGKTFVEWSARSFDP
                     RGRVTAYREVFDGGLGRSVKEWSGSYDDRGRLAASREEREDVSGMRTITDLKQIQYDS
                     RGRMTDSFQTLRCSDRPSLFTEVRLRGRSYNEQGQVTAGIEETMIFGRDEGHEINFVI
                     TKKIEDVRQGAGGLIACVERTETVGVDDAGRSIRREERSEIKYDDGLTRQRNIQRAYG
                     EDGAVGLETIREVRCLERARDLQGRVVLQRDEITDDAAPSEKMIVVRRNFWGAEGTLD
                     GYEELSESRGQTVRRRVSETAYDAAGRLVAGKETVVRPESASVRTETRKSKISYDIFG
                     RAVSWEENTEIFGPGLAHSMRSVKESTFDECGRTVSQSEEIHRGGDVFLKRSDYHAFD
                     GFGRAVSFSETGSDENGPFQTTRSGIRYDSFGRVSGYVEEGISSGAWKKRTWAVLEFD
                     ERDREQRTMEEGFGDSGSYRLETEIEGVNTLGQTTGRLEKGWSAAEGDFERRFSDMRY
                     DSAGRVQEYDLLKRAVGEKTVSHWGGLGYDEKGRSLGYMEVGDVWENGEFARRFENQW
                     RVRGFSSENGMVSDSEQIEYREYRGGEIEKQIRTRKNAAYDPLGRLTGYEDTETSERT
                     EAGETQTATVLRRRENIGYYESSGRDGGLWPGRVKGYQETLVDRTAVHHVTQTRVEEI
                     QYDADGRQTASRTFSAGKGLDDGSADEPSTAGWIEKVKKFFLKWSAVAESRVVQENVS
                     GGFVDRPLADLTEDLPVDGFILTRRLDTRYDVQGRPTEWMEVSASAAAAEKAAVSLVQ
                     VGYQGDSRRLAFYSARQEEGTKVQHIFKDGFSYGNSAQAGSFRECVFEGDRLRFLGKE
                     FDWRAASWEAKSCFMDELSAGRREVEGAVQITRYDDVRYDAQGQMLDSCGVRTTRGVV
                     LVAADGEADLAVTLDQMRDDEARRIDAEIDVELDREKKSLDEAAQEVWDKKLQAM"
BASE COUNT         1498 a         1928 c         2467 g         1385 t
ORIGIN      
        1 cgcgcccatg accctggtcc tgctctattc ggcgggggcg cccggcggcg tgcttctgtc
       61 ggtggcggtg ttcggcttcc tggcgctggg catccccctg gcctccactt atttcaagtt
      121 ggtcggtgac cggtcccagg attcctggct gtgggggggg atgcggtctg ttttcctgga
      181 gcgccgcgcg ttcttcctgt tctggagcgg cgtctgtctg tccttggcgg ggggatggtg
      241 gttcctgcgg aagtggcgcg tgttcatccc gtcgctcacg cttttctgtt cgttggcggt
      301 ggtcgtttcc ggcgtggcgg gctccttcgc ggtcaaaaag gccctcaagg attaccagcg
      361 taagcggctc atcgcgttcg tggatcccag cgtcgatccg ctcagcgccg ggtacaacat
      421 ccttcaatcc gagatcgcca tcggatccgg aaggttcttc ggtaaagggt tcctgggcgg
      481 tagccagacg cagttggggt ttcttccgga aaaacacacg gacttcattt tctcgctgtt
      541 ggccgaggag tggggattca tcggcgcgtt gatcatcttg ctcctttatt ttttcgttgt
      601 ctggaggtct ttcgagatcg ccctcgccgc ccgggaacgg ttcggccgct acctcgccgt
      661 cgggctcggc accatttttg ctttcaccgg aatcgtgaac atcggcatgg tgatgggatt
      721 gatgccggtc accggcctgc cgctcccctt cgtttcctac ggaggttcgt cgttggtggg
      781 ggcgtatctg gccgtcgggc tgctgctgtc gatccatctc cgacgttacg tcctctgaac
      841 gggacgcgtc ggcgcggttc caatgaaaat tcacgcgtta tcacatcgga ggagaaaatg
      901 aaaaaggaaa tcatcgtcaa tcacacgccg gaggagatcc gcgtggccgt catggaggac
      961 ggccgcctgg tggaatacct catcgagcgt ccggccctcg gtatggccaa actggtcggc
     1021 aacatctaca aggggcgcat cgagaacgtc ctgcccggca tctccagcgc tttcgtcaac
     1081 gtcggctacg agaagaacgc ctatctctac gtcacggacg tcgtctccga cgccaaggag
     1141 cggcacatcg aaaagatgct gcagcggggg gaccccatca tggtgcaggt ggccaaggag
     1201 gccatcggca ccaagggcat gaaggtcacc atggacatct ccttgcccgg ccgctatctc
     1261 atctacatgc ccaagagcga gcacctgggc gtctccaaga acatcgaaga cgccgctgag
     1321 cgcgaccgtc tccgtcgggc catcgagaag accaacccca tcggcggcgt catcgtgcgc
     1381 accgaggcgg agggggcgga ggagcgggaa ctcgcccacg agatgcggta cctcatgcgc
     1441 ctctgggaaa acatccagaa aaaatacgaa tccaccgcgc cgggcgccct catccatcgg
     1501 gaactcggcc tcaccttcca ggtggcgcgc gacctgctca cggacaacgc ccacatcttc
     1561 ctggtggatt ctcaggagga gttcaaggac ctgcgggggt tcgtcgagat gctcgcgccc
     1621 gagctggccg accgcgttca gcactacacc ggccgcaccc ccatcttcca ggccttcggc
     1681 gtggaaaagg agctcagcca actgcgccac cagcgcatca atcttccgtc gggcggctac
     1741 atcatcatcc aggaggccga gtccctctgc gccatcgacg tcaacaccgg caagttcatc
     1801 ggccacaagt cgcaggagga gaccgtcacc gccaccaacc tggaggcggc cgcggaggtg
     1861 gcccggcagc tgcgtctgcg caacatcggc ggcatcatcg tcatcgactt catcgacatg
     1921 cgccgccgcc gcaaccagca caaggtggtg gaggcgctcc agcacgccac ccgcaacgac
     1981 cgcgccaaga tcaagatcct gcccatcacg cgcctcggcc tcgtcgaaat gacgcgcgaa
     2041 cggcgccggg agtccctcct ttccctcatg accgaaccct gccgtgaatg cgacggcagc
     2101 ggccgtgtgc tctcgcgcga gtcgctctat ctcaagatcc agagcgagat cgaggacctc
     2161 accaagggcc gcaccgacgg caccctcaag gtctcggtgc cgccggtggt cggcgacttc
     2221 ctgcgggaac gccaggagcg tttggaaaaa aacctccagc gctccatcga ggtccagatc
     2281 gaccccaccc tcccgtggga ggattaccgc atcctcatcg agtagttttc cctcctggga
     2341 gggcggacgg attccgccct cccaggggcg ctgtctcctt ccgaaatccc ggttcgccga
     2401 gataaagaaa atttgtcata attgtcaaag tggtatatga gggaacgtga ggcctgcgag
     2461 gcggcctcgc gttttttgtt tttgcgggac aggataaccc tccatccccg atggccatgg
     2521 tggatatgtg aaccggatga gtttggcccg cctaaaaaaa atcgtcgccg gctttgtcgg
     2581 tttttccatt attttcacgt ctctcccgag acccccgctc ttcgctgctt ccccccgaaa
     2641 caaagaaacg aaacataaaa catacgggaa ttattcggtc gagtcgcttg aatcctctgc
     2701 gttttcgccg gaacaattgg agaaaagcat cgaaaggcag gaggctttcg tgtctttcca
     2761 caacgagcgc ctttctcagg ggaaaaggga ggatcccgcg gcgatgttgg acaggcttat
     2821 acgcgacgcg gagttcggga gattcatctc cggagaggaa tcgtcggagg aggtccaacg
     2881 ccgccgaagg gcggtgtccc ggctcctgtc ggtttctggg catcgatata agattcatgc
     2941 ggatggtcga aaggtatggt tcaagggggg ccgggtcagc cggatcgaga atgagccgat
     3001 ccttcaggaa tccggacagg tctcccggcg cgataccacg gagatgcttt acgacgaaca
     3061 cggaaattta acgtcctata ggacggaaac gcgcaacgcg gatggggaga ggctgtgtgt
     3121 ccagtgggcc ggccgttacg ggccgagtcc gggcggcaaa tatcagctgc agggttatat
     3181 agagacgcga tgggatgctc tgggaaatgc gtcccggctt gaacggtcgc aggtccggtg
     3241 ggacccggcc ggccgtgcgg ccgcgtatcg cgagacggag accgatccgt gggggcggca
     3301 aacggttcgg gagatgtcgg gcatccgcta tgattccaac ggccggccaa cgtcttatcg
     3361 ggaaaggatc atcgggtctg acggcggcac gcaggagcgg tggcgggtgg aaacgcgtta
     3421 cgaccgtctt gacggtcggg aagaggcggt ttccttttat agggaaattc gggggacggc
     3481 ggatggcggg ggcgatgagg tggaatggtc ggccgaaaaa tacgacaggc tcggtcgcgt
     3541 ccgggcctgg acagaaaaga cgaccgattc ctccggcggc gtcactcttt gccggcggag
     3601 cgatattgag ttcgaccgga acggcaacgt gacggggttc gaagagaacg aaaacggccc
     3661 agccgggagc cgtcgcagga tatggagcga cggggtatat gaccggtggg gccgcgtggt
     3721 ctcttacagg gaaattatcg tgggaggcga ccagaacgtc caccgtcgat ggggcggggc
     3781 cgaatataac gcaaaaaacg agttgttgtc tttcgcggag gagttgaccg atgcccgcgg
     3841 acgaacaacg cagagtcggt ggaaagcatt ggaatatcaa accgggttcg tccgggatta
     3901 tcaggaaacg acgcgcgtgg atgacggagg agaggtctct caccgttggc gaggccggca
     3961 cgacgccggc ggacgagtga cggggttcgt ggatgaaagg atcgacccgg agggaaatcg
     4021 ggaggtcagc gaaaggaatg ggataaccta cgaccgggcc ggccggttgg cgggttttca
     4081 tgaggcaaga acggacaatt tcggaaacaa aacgatccga acatggacgg cccagaggta
     4141 cgacgcgtac gaccgggtcc tcgattatga agagatggaa accggggcct ttggcgagga
     4201 aagaaaaagg gtctggaaaa atgctcaata cgacgctcag gggaggctcg cctcttacga
     4261 ggaagatgtg cgggtggacg atcgctggat ccaacgtcga tggcgggcgg acgcgtacga
     4321 ttcgttcggt caagtcaccg tgtttcatga ggaaacgagc gatcccttgt gtgacgaaac
     4381 gttccgttat tggcaggggc gatacgatcg ggagggacgg ttggttttca gccgtgaaga
     4441 gaacgtggag gggacggggg gtaaaacgtt cgtcgagtgg tccgcccggt ctttcgatcc
     4501 ccggggacgg gtgacggctt atcgggaggt cttcgacgga ggcctgggga gatcggtgaa
     4561 ggagtggtcg gggtcttacg atgatcgcgg acggttggcc gcttctcggg aagagaggga
     4621 ggacgtctcc gggatgagaa cgatcacgga ccttaagcag atccaatacg attcgagagg
     4681 gcgcatgacc gattcttttc aaacgctccg atgttccgac cggccgtccc tttttaccga
     4741 ggtgcgcctc cgggggcgtt cctataacga acaggggcag gtcaccgccg gcatcgagga
     4801 aacgatgatt ttcggcaggg atgaggggca tgaaatcaat tttgtgatca caaaaaaaat
     4861 cgaggacgtc cgccaggggg cgggagggtt gatcgcttgt gtcgagcgga cggagaccgt
     4921 gggggtggac gacgccggcc gctctatccg tcgtgaggag agaagcgaaa tcaaatacga
     4981 cgacggcctg acgcggcaga gaaacattca gcgggcctac ggggaagacg gcgcggtcgg
     5041 cttggagacg atacgggaag tgcgttgcct ggaacgggcg cgggaccttc aggggcgggt
     5101 cgttttgcag cgggatgaaa tcaccgatga cgcggcccct tccgaaaaga tgattgttgt
     5161 tcggaggaat ttctggggag cggaggggac gttggacggg tacgaggaac tttcggaatc
     5221 gcggggacag acggtccgtc ggcgggtgtc cgaaacggcc tacgatgcgg cggggcggct
     5281 tgtcgccggc aaggagaccg tcgtgcggcc ggaatcggcg tccgtccgca cggagacccg
     5341 gaaatcaaag atctcctacg acattttcgg gcgggcggtt tcttgggaag aaaatacgga
     5401 gatttttggt ccggggctgg cgcattccat gcgcagcgtc aaagagtcga cgttcgacga
     5461 atgcgggagg accgtttctc agtcggagga aatccaccgc ggcggggacg tttttctgaa
     5521 gaggtcggat tatcacgctt tcgacgggtt cggacgtgcc gtttcctttt cggaaacggg
     5581 cagcgatgaa aacgggccgt tccagacgac ccgttccggc atccgctacg attcgttcgg
     5641 tcgggtttcc ggttatgtcg aggaagggat ctcctccggc gcctggaaaa agaggacgtg
     5701 ggccgtcctt gagttcgatg agcgggatcg cgagcaaagg acgatggaag aggggtttgg
     5761 ggattccggg agttatcggc tggaaaccga aatcgaaggg gtcaacacct tgggacaaac
     5821 caccggccgg ttggaaaaag gctggtcggc cgcggaaggg gattttgaac gccggttttc
     5881 ggatatgaga tacgactccg ccgggcgagt gcaggaatac gatctgttga agcgcgccgt
     5941 cggggaaaag accgtctccc attggggtgg gctgggctac gacgagaagg ggcggtctct
     6001 gggatacatg gaggtggggg acgtgtggga aaacggcgag ttcgcccgtc gttttgaaaa
     6061 ccaatggcgc gtccggggtt tttcatcgga gaacgggatg gtctcggact ctgaacagat
     6121 cgaataccgg gaatatcgag gaggagaaat cgaaaaacaa atccggaccc ggaaaaatgc
     6181 cgcgtatgat ccgctcggtc gcttgacggg gtatgaggac acggaaacga gcgagaggac
     6241 ggaagccggg gaaactcaaa cggcgaccgt cctccgccga cgggagaaca tcggatatta
     6301 tgagtcgtcg ggacgggacg ggggcctctg gccggggagg gtgaaggggt atcaggaaac
     6361 cttggtcgac cggaccgccg ttcatcatgt aacacagacg cgcgtggagg agatccaata
     6421 cgatgccgac ggccgacaga cggcttcacg gactttttcg gcgggaaaag gtctggacga
     6481 cgggtcggcg gacgaaccgt cgacggccgg ctggatcgaa aaagtgaaga aattcttttt
     6541 aaagtggtcg gcggtcgcgg agtcgcgtgt cgtccaggaa aacgtttccg gcgggtttgt
     6601 cgacaggccc ctcgccgatt tgaccgagga tcttccggtt gatggtttca tattaacccg
     6661 ccgcctcgac acgcggtatg acgttcaggg gagacccacg gaatggatgg aggtgtcggc
     6721 gtcggccgcc gcggcggaga aggccgcggt ttcgctggtt caggtgggtt atcagggaga
     6781 ttcccgccgg ttggcgtttt attccgcccg ccaagaagag gggacgaagg ttcagcatat
     6841 attcaaggac ggttttagtt acggcaattc cgcgcaggcc ggttcgttcc gggaatgcgt
     6901 tttcgaaggc gaccgtttgc ggtttttggg aaaagaattc gattggcggg ccgcgtcctg
     6961 ggaggcaaag tcctgcttta tggatgagct ctctgcgggg cggcgggagg tcgagggggc
     7021 ggtccaaata acccggtatg acgatgtccg ttacgatgcg cagggccaga tgctggattc
     7081 ctgcggcgtc cggaccaccc gcggcgtggt gctggttgcc gccgacgggg aggcggattt
     7141 ggccgtcacg cttgaccaga tgcgggacga cgaagcgcgg cggatcgatg cggaaatcga
     7201 tgtcgagctc gatcgggaga aaaagtcttt ggatgaggcc gctcaagaag tgtgggataa
     7261 aaaacttcag gccatgag
//
LOCUS       DAOTFD010000126         7258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7314_length_7258_cov_4.687075, whole genome shotgun sequence.
ACCESSION   DAOTFD010000126 DAOTFD010000000
VERSION     DAOTFD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7314_length_7258_cov_4.687075"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="P5079_07725"
     CDS             complement(<1..1711)
                     /locus_tag="P5079_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY29910.1"
                     /translation="MTRIQPDDLYRTLAEATLDKVYIVDRNGVVLHANTSAAQCFSTK
                     PAELVGKSLSVLCPPEKSEKYLKSIREVFETGRPLAIGLSDALHGNEVGEQSFLSPVK
                     NKKGRIAAVLVTSSGDGRDARAAEALRESGMLYQDLANNPSAGVYRIRVRHLSPWSAP
                     EAPPYVYEFVNDRYCELTGIPAEKHYSDPTVTLRQIHPDEYENWAALNERANRTGEPF
                     FWEGRVVVKGQLRWFHYESRSRSLPNGDLLWTGILLDITERKQLESAVELIAKKYSTV
                     FDNVSDGMWIIDLRGRIVEVNDSYCRMSGYRRSELIGMHIKKLEGLESAAAIAAHMKK
                     IIECGQDRFETRHRRKDGSHIALEVVTLSMALEGGRIAMLARDITKRKRAEEELRNSE
                     QRYRGTLNLLGDMIHVVDAGLNVVLANQVLRQLNRRLGLREDFIGENLFKVYPFLTAK
                     VRREYEKVIATGEPLVTEELNRIAGAEFSTETRKIPIFEGGRVKQVLTVMRDVSERLR
                     AQKNQERLQGEILSALTRQQIMIGQTLHDSLGQLLTGISYMVKGLEHDLKRGKPPAAK
                     ETAR"
     gene            complement(2156..2226)
                     /locus_tag="P5079_07730"
     tRNA            complement(2156..2226)
                     /locus_tag="P5079_07730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2192..2194),aa:Gln,seq:ctg)
     gene            complement(2521..2826)
                     /locus_tag="P5079_07735"
     CDS             complement(2521..2826)
                     /locus_tag="P5079_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRY29911.1"
                     /translation="MVTAVVLINVERAQVNEVAEALLELKGISEVYSVGGRYDLVAVI
                     RASDNEQLAALVTREMLKLKGISKTETLIAFRAYSRHDLESMFSVGMEEVPPSKKRA"
     gene            complement(3015..3653)
                     /locus_tag="P5079_07740"
     CDS             complement(3015..3653)
                     /locus_tag="P5079_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29912.1"
                     /translation="MIQGRKNPVTPRPFSGPLGVFLCLSLLLGPQVSFSGTLSPISRT
                     VIGLHLGMSETAFLKYHRAELVSQDPEFRQKVYTLRYITDTDVRQVTVNFYNKKLLKL
                     DLEFTDSFVRTVEWFELVKRFNAKYGVSRITQMETPPHITDLLEWDDSRTHLVLRHVW
                     QPDASAGELRSVYRLSYVDADYYVETLGVRAPMARLPQEGSFSSDSSYYTRD"
     gene            complement(3730..5460)
                     /locus_tag="P5079_07745"
     CDS             complement(3730..5460)
                     /locus_tag="P5079_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRY29913.1"
                     /translation="MVAPKNEPENSSGKAAKAAASDFIRDIIDEDLRSGRNGNRVHTR
                     FPPEPNGYLHIGHAKSICLNFGIARDYNGKCNLRFDDTNPAKEDQEYIDSIKQDVQWL
                     GFDWEGREFYASDYFEQLYRWAEQLIQKGKAYVCDLTGDEVRQYRGTLKEPGRESPFR
                     NRSVEENLDLFRRMRAGEFPDGARTLRAKIDMAHPNLNMRDPVMYRILRATHHRTGDR
                     WCIYAMYDWAHGQSDSIEKITHSICTLEFEDHRPLYEWFIRELGIPAPRQIEFARLNL
                     TYTVMSKRKLLELVKGGFVKGWDDPRMPTLSGLRRRGYTPESIREFARMIGVTKSNST
                     VDFAALEYCLRQDLNKRAPRRMAVLRPLKVVIDNYPEGKSEELDCVNNPEDAAAGTRR
                     VPFSKTLYIEQDDFREEPPPKFFRLSPGREVRLRYAYFVKCVGVVKDPVTGTVTEVHC
                     TYDPATRGGDSPDGRKVKATLHWVAAGHAVPVEARLYENLFLTPDPDDVPEGKDYKDN
                     LNPRSLEVLAPCYVEPSLKSAKPGEKFQFERLGYFCADRDSTPERTVFNRTVTLQDAW
                     AKIEKKQRPA"
     gene            complement(5463..6950)
                     /gene="gltX"
                     /locus_tag="P5079_07750"
     CDS             complement(5463..6950)
                     /gene="gltX"
                     /locus_tag="P5079_07750"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY29914.1"
                     /translation="MKTRVRFAPSPTGFLHIGGARTALYNWLFARNTGGTFILRIEDT
                     DETRSTQESVDAIFKGMRWLGLDWDEGPLSASDPNAHSGDHGPYFQMQRLDGYRAAAD
                     RLIAEGKAYPCYCTAEEVDAMREKALKEKRNPRYDGTCRRLTEAQRRERAAQGRTASV
                     RFSAPPEGSVEFDDLIRGPMKFDNEQIEDFILLKTSGVPTYNFACVVDDHAMEITHVI
                     RGDDHLSNTPRQVLAYRALGWTPPVFAHLSMILGADGSRLSKRHGATSVEEYRQAGYL
                     PEAVLNYLALLGWATSDSQDIFGKEELVAKFDVGRCSKSPAVFDPNKLIWMNGEYLRK
                     MPAKELAVRARPFVASAGLLDDAGLAARAAELEVAVAMEQEKVKLLTDVPKRIDIFFK
                     DVAYDPEAVEKVLKKPDAAAVLEDAIKTFQALEPFTAASTEEACKALAARRGVKNGAV
                     FHPVRVAVSGRTQGPSLFHMLELLGKQKSLERIQKTLQTFFKNAG"
     gene            complement(6973..>7258)
                     /locus_tag="P5079_07755"
     CDS             complement(6973..>7258)
                     /locus_tag="P5079_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRY29915.1"
                     /translation="WKRQKIENLAILLKGALAAEGMVGLKMNLKEEDLGKIVELLPAL
                     KNPTISRLTLPGWIAIEVIMAEKDVKQRLPALKRAGAEGIIEYPLNKVIY"
BASE COUNT         1267 a         2310 c         2163 g         1518 t
ORIGIN      
        1 tgcgcgccgt ttctttggcg gcgggcggtt tccccctttt aaggtcgtgt tccagccctt
       61 tgaccatgta ggagatgccg gtcaggagct ggccgagact gtcgtggagc gtctgtccga
      121 tcatgatctg ttgacgcgtc agcgcgctca atatctcccc ttgcaggcgc tcctggtttt
      181 tctgcgcccg caggcgttca ctgacgtccc gcatcacggt caggacctgc ttgacccgcc
      241 ccccctcgaa gatggggatc ttgcgcgtct ccgtcgaaaa ctcggccccg gcgatgcggt
      301 tcagctcctc ggtcacaagc ggctcccccg tggcgatgac tttttcgtat tcgcgccgca
      361 ccttggccgt caagaacgga tagaccttga ataaattttc cccgatgaag tcctcccgaa
      421 gccccagccg ccggttcagt tggcgcagca cctggttcgc aagaacgacg ttcaaaccgg
      481 catcgaccac gtgtatcatg tcccccagca aattgagcgt gcccctgtag cgctgttcgg
      541 aattgcgcag ttcctcctcg gcgcgcttgc gtttggtgat gtcccgcgcc agcatggcga
      601 tccgcccccc ctccagggcc atggacagcg tcaccacctc cagggcgatg tgcgaaccgt
      661 ctttccgtcg atgccgggtc tcaaaacggt cctgcccgca ttcgattatc ttcttcatat
      721 gggcggcgat ggccgcggcg gactcgagtc cctccagctt cttgatatgc atgccgatca
      781 actcgctccg ccgatagccg gacatccggc agtaggaatc gttcacctcg acgatccttc
      841 cccgaaggtc gatgatccac atcccgtcgg aaacgttgtc gaacacggtc gagtattttt
      901 ttgcgatcaa ctcaaccgcc gattccaact gtttccgttc ggtgatatcc aaaaggatgc
      961 ccgtccaaag caggtcgccg ttcggcaggc tcctggaacg cgattcataa tggaaccatc
     1021 gcagctggcc cttcaccacc acgcggcctt cccagaaaaa aggctccccg gtccgattgg
     1081 cgcgttcgtt cagggcggcc caattttcat actcgtccgg atgtatctgg cgcaaagtga
     1141 ccgtcggatc cgaatagtgt ttctcggcgg ggatcccggt caattcgcag tatcgatcgt
     1201 tgacgaattc atagacataa gggggcgcct cgggcgcgct ccacggagaa aggtgtcgga
     1261 cgcggatccg gtaaacgccc gcgctcgggt tgttcgcgag gtcctgatag agcatcccgc
     1321 tctcccgcaa cgcctccgcc gcccgcgcgt cccggccatc gcccgacgac gtcacgagga
     1381 cggccgcgat tcgccccttt ttatttttaa cgggcgacaa gaaggactgc tcgccgactt
     1441 cgttcccgtg caacgcgtcg gacaggccga ttgccagcgg ccggccggtt tcgaaaacct
     1501 cgcggatgga cttgagatat ttctccgact tctcgggtgg gcacaacacg gagaggcttt
     1561 tgccgacaag ttccgccggc ttggttgaaa aacattgggc cgccgacgtg ttggcgtgaa
     1621 ggacgacgcc gttccggtcg acgatgtaaa ctttatcgag cgtcgcctcg gccagcgtcc
     1681 gatagagatc gtccggttga atccgcgtca cggtttttcc ggctccgact ttggaaaggg
     1741 tccccgtcat ttacggaatc cagggcatcg gcccatggcc ccgggacgtc ccgcgtccga
     1801 ggaggaagaa aagcggtttc cctctgttcc ggctctccgc gggaacgctc ccgtcgtcat
     1861 acaaatcaaa aggctccccc cgggggagcc tttcacttta acatcgcttg gttttcgata
     1921 gaacgccctc gccatccctt cactccccca cttcgtcaga tccgttatcg aactccattt
     1981 cttcgaaaaa aacagaaggg gcccgaagga acccttctgt tttgacccgt caggagggcg
     2041 gcccctcctc gggggaacga agtcccgctt ccttcgggac gtattcgccg cccatccgcc
     2101 gcaatccctc gattccatca cgaaccggag agatttaagg aggggttcgg aaattctgcc
     2161 ccgctaggat ttgaacctag actaagtggt tcagagccac tcgtgctacc gttacactac
     2221 ggggcaatat cggcggctcc catgccgcgc cgtctcacgg ctcaccgtgc ttctggctcc
     2281 gcagacatcg cttcgccgag ctccacgatt cgcccgtcag gaagggctcc gccctccctt
     2341 gacgccaacg ctgaccctcc caaacaggag gaaatcgttt cagaaactct tatccgatat
     2401 atcggggaca acacgcgttt ccgtcacggt ttgcgcgtta tcgcgctccg cccatttccc
     2461 ggcaaaacct gcccggcaaa cggcaccgcc ggcccttcgg attcgcctcc ccagaccccc
     2521 tcaggcgcgt ttttttgagg gagggacttc ctccatccca acggaaaaca tcgattccaa
     2581 atcgtgacgg gaataggccc gaaaggcgat cagcgtttcc gtcttcgata tccccttcaa
     2641 cttcagcatc tcccgggtca ccagcgcggc cagttgctca ttgtctgacg cgcggatcac
     2701 ggccaccaaa tcgtaacggc cgcccaccga atagacctcg gaaatgcctt tcagctccag
     2761 caaagcttcg gccacctcgt tcacctgcgc ccgctccaca ttgatcaaga caacggccgt
     2821 caccatatcg ttcactcctc tgagaatgtc cgtcccggcg attttaccag tttttttctc
     2881 cggggggcaa tcctcgcccc tccggcccac gcccccccct ttttcgccca accggttccg
     2941 gcggcaaatg tccggcataa aaaatatccc cgggcgccga gccccggggt tccgtttccc
     3001 ttcagtggtt gaaattagtc gcgggtgtag taggaggaat ccgaggagaa cgaaccctct
     3061 tgaggcaggc gggccatggg ggcccgcacg ccgagcgtct ccacgtaata gtcggcgtcc
     3121 acgtaactga gtcgatacac ggagcggagt tcgccggcgg aggcgtccgg ctgccacaca
     3181 tggcgcaaca ccagatgcgt gcggctgtcg tcccattcca acaggtcggt gatgtgcggc
     3241 ggggtttcca tctgggtgat gcggctcacg ccgtatttgg cgttgaaccg cttcaccagt
     3301 tcgaaccatt ccaccgtccg gacgaaggaa tcggtaaatt ccaggtccag cttgaggagt
     3361 ttcttgttgt aaaaattgac ggtcacctgg cgcacgtccg tgtcggtgat gtagcggagg
     3421 gtgtaaacct tttggcggaa ctcggggtcc tgggacacca gttcggcccg atgatattta
     3481 aggaaggccg tctcgctcat gccgagatgc aggccgatga cggtgcggct gatggggctg
     3541 agggtgccgg aaaaggagac ctgagggccg agaagaaggc tcaggcataa aaaaacgccg
     3601 agcggccctg aaaacggtct cggcgttacg gggttctttc taccttgaat cacgatagca
     3661 taatagtatg atgcggaacc gaattttgtc aagggttttt taaaaaaatc cgggaagacg
     3721 gtggatcgct caggcgggcc gctgtttttt ttcgattttg gcccaggcgt cctgcagggt
     3781 gacggtgcgg ttgaaaacgg ttctttccgg cgtgctgtcc cggtccgcgc agaaatagcc
     3841 cagccgctcg aactggaact tttcccccgg tttggcgctt ttcagggaag gttccacgta
     3901 gcagggggcc aacacctcca gggagcgcgg gttgaggttg tccttgtagt ctttcccctc
     3961 gggcacgtcg tccgggtcgg gcgtaaggaa gaggttctcg tacaaccgcg cctccacggg
     4021 cacagcgtgg ccggccgcca cccagtgcag agtggccttc accttgcggc cgtcgggcga
     4081 atccccgccg cgggtggccg ggtcgtaggt gcaatgcacc tcggtcaccg ttccggtgac
     4141 cgggtccttc accacgccca cgcatttgac gaaataggcg taccgaagac gcacctcgcg
     4201 gcccggcgac agacggaaga atttgggcgg cggctcctcc cggaaatcgt cctgttcgat
     4261 gtagagcgtt ttcgagaacg gcacccggcg cgtccccgcg gcggcgtcct ccgggttgtt
     4321 cacgcagtcc agttcctcgc tctttccttc cggatagttg tcgatcacca ccttcagggg
     4381 ccgcaacacc gccatgcggc gcggcgcacg cttgttgagg tcctgccgga gacagtattc
     4441 cagcgcggcg aaatccacgg tgctgttgct cttggtcacc ccgatcatgc gggcaaactc
     4501 acggatggac tccggcgtgt atccccgccg gcgcaggccg gagagcgtgg gcatgcgcgg
     4561 gtcgtcccac cccttcacga atcccccctt caccagttcc agcagtttgc gcttgctcat
     4621 cacggtatag gtgaggttga gccgggcgaa ctcgatctgc cgcggcgcgg ggatgcccag
     4681 ttcccggatg aaccactcgt agagcggccg gtggtcctcg aactccagcg tgcagatgga
     4741 atgggtgatc ttctcgatgg aatcggactg gccgtgggcc cagtcataca tggcataaat
     4801 gcaccatcgg tcgccggtgc ggtggtgcgt ggcgcgcagg atgcggtaca tcaccgggtc
     4861 gcgcatgttg aggttgggat gggccatgtc gatcttggcg cgcagggtgc gggccccgtc
     4921 ggggaactcc cccgcccgca tgcggcggaa aaggtcgagg ttctcctcca cggaacggtt
     4981 ccggaacggg ctctcccggc cgggctcctt gagcgtgccc cggtattggc gcacctcgtc
     5041 gccggtcagg tcgcagacgt aggccttgcc cttttggatg agctgttccg cccaccgata
     5101 aagctgttca aaataatccg aggcgtagaa ctcgcggccc tcccaatcga accccagcca
     5161 ttggacgtcc tgcttgatgg agtcgatgta ttcctggtct tccttggcgg ggttggtgtc
     5221 gtcgaaccgg aggttgcatt tgccgttgta gtcgcgggcg atgccgaaat tgaggcagat
     5281 ggacttggcg tggccgatgt ggagatagcc gttgggttcc ggcggaaaac gggtgtgtac
     5341 ccggttcccg ttccggccgg aacggaggtc ctcgtcgatg atgtcccgga tgaaatccga
     5401 ggcggcggct ttcgccgctt ttcccgagct gttttcaggt tcgttcttcg gcgcgaccac
     5461 ggtcagcccg cgttcttgaa aaaagtctgc agcgttttct ggatcctctc cagacttttt
     5521 tgttttccca aaagttccag catgtggaag aggctgggcc cctgcgtgcg gccggacacc
     5581 gccacccgca cggggtggaa caccgccccg ttcttcacgc cccgccgcgc cgccagcgct
     5641 ttgcacgcct cctcggtgga ggcggcggtg aacggctcca acgcctggaa cgtttttatc
     5701 gcgtcctcca gcacagcggc cgcgtccggt ttcttcagca ccttctccac ggcctccggg
     5761 tcgtaggcca cgtccttgaa aaaaatgtcg atgcgtttgg gcacgtcggt gaggagtttc
     5821 accttctcct gttccatggc caccgcgacc tcgagttccg cggcgcgcgc cgccaggccg
     5881 gcgtcgtcca gcagccccgc cgacgccacg aacggccggg cccggacggc cagctctttg
     5941 gccggcatct tccgcaggta ttcgccgttc atccaaatga gtttgttggg atcgaagacg
     6001 gccgggcttt tgctgcaccg ccccacgtcg aacttggcga cgagttcctc cttgccgaaa
     6061 atatcctggc tgtcggaggt ggcccagccc agcagggcga ggtagttcaa aacggcttcg
     6121 ggcagatacc ccgcctgccg gtattcctcc acgctggtgg cgccgtgccg tttggaaagg
     6181 cggctgccat cggccccgag gatcatcgac aggtgcgcga acacgggggg cgtccagccg
     6241 agggcccggt acgccagcac ctgacgcggc gtgttggaaa gatggtcgtc cccgcggatc
     6301 acgtgggtga tctccatggc gtggtcgtcc accacgcagg cgaaattata ggtgggcacg
     6361 ccggaggtct tcagaaggat gaaatcctcg atctgctcgt tgtcgaactt catggggccg
     6421 cggatgaggt cgtcgaactc cacggaaccc tccggcggcg cgctgaaccg cacgctcgcc
     6481 gtgcgcccct gggccgcgcg ttcccggcgc tgcgcctccg tgagccgccg gcaggtgccg
     6541 tcgtaccgcg ggttcctctt ttccttgagc gccttctccc gcatggcgtc cacctcttcg
     6601 gcggtgcagt aacacgggta ggccttgcct tcggcgatga gccggtcggc ggccgcccgg
     6661 taaccgtcca gccgctgcat ctggaaatac gggccgtggt ctcccgaatg cgcgttcggg
     6721 tcggaagccg acagcggacc ttcgtcccag tccagcccca gccagcgcat tcccttgaag
     6781 atcgcgtcca ccgattcctg cgtggagcgc gtctcgtcgg tgtcctcgat gcggagaatg
     6841 aaggttcctc ccgtgttccg ggcgaagagc cagttgtaaa gcgctgtgcg ggcgccgccg
     6901 atgtggagaa accccgtcgg cgacggggcg aaacgaacgc gcgttttcag gcgagattcc
     6961 gttcggagaa ggtcaataga tgactttgtt caggggatat tcgatgatgc cttcggcgcc
     7021 ggcgcgcttg agcgcgggga gccgctgttt cacgtccttc tcggccatga tcacctcgat
     7081 ggcgatccag ccgggcagcg tcaggcgcga gatggtgggg ttcttcaacg ccggaaggag
     7141 ctccacgatc ttgccgaggt cctcctcttt caggttcatc ttcaacccca ccatgccctc
     7201 ggccgccaag gcgcccttca gcaggatggc gaggttctcg atcttctgac gtttccac
//
LOCUS       DAOTFD010000127         7257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7316_length_7257_cov_3.474452, whole genome shotgun sequence.
ACCESSION   DAOTFD010000127 DAOTFD010000000
VERSION     DAOTFD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7316_length_7257_cov_3.474452"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2846
                     /locus_tag="P5079_07760"
     CDS             <1..2846
                     /locus_tag="P5079_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY29916.1"
                     /translation="PFLPAWPVFSRGASYYWIGGLSFVFIFAAFFALWRLKWVPGIAL
                     LLLGGFSLGASRYFQTTDLTNPHHISHLAGDSYDKAVILRGTIIQDPDYRERYVNVTL
                     QPEEVVLDPKKPEERIRLFGNTGLVRATIYPNVGNYYWRVSYGDRVELAMPLMKTRDL
                     TNPSGFNFGRYLRARGVYATAPPWGIRQPGHIRYLGRGRVSFLVKLSLDLKLNLLSSI
                     RRTMPYPESAFLGGVTLGLRAGLPQKIKLQFQETGVGHVLAVSGLHVGFVHALLLMIC
                     RVFRIHRRISYFVIVFGLIVFAIITGASPATQRAVLMSSIGQAIYTFGGLGIRGSTVM
                     TIPLAGMILLLLNPTIAPDGSFVLSIVAVWSLAHLSGAMVRLLEIFGRGWKFLAGLFF
                     MLVWTGIAVLDAAAFTRAPAWISLGLVSAAAVAAAFYLDRKYPMPGVSLFVLPGWFTG
                     LAAAQGAILLGMTFPLSAMFFQRYSVSGTYANFIAIPLIGFIVQLGLLAGLLELIFTS
                     VGLPAVGTYIALCINASNWYLCQLFLLVTAFFSKHFPYPYVPMPSVSQRVIFYVLVMG
                     FVFWKPLTQWAQSLLEWARTPRRRWAVVGVFTLLIGSIAARTTAFSEMHGKMRVLFFD
                     CGHGNSILIRTPHGKAILIDGGPSGRGGWNTGETVLAPVLAKFNVKNLETVVLTSPRE
                     SQGGGLPFVLDHFTVNKIFSTLDGRYFSPRMTFGQFLRALGDRYLINNRHDKESHEAF
                     TMMHDLFVMDVFRAKMEKASAGDVLYEEEAGGKKLILRALWPLAAPLRNTKDDVGNNS
                     MVLKLTYGEVSFLFVTQLRQEGEWALLDAAGDELKSDWLLVPANGNIEASSPEFLEKV
                     APKNAVMQTGYVGWRDRRRYHVATKQLDALVERYTRRGITLYRTDVSGAVTVTTDGED
                     SQSETVLPVLPTISAPLSPSDREARDKDLEIFL"
     gene            2846..3799
                     /locus_tag="P5079_07765"
     CDS             2846..3799
                     /locus_tag="P5079_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY29917.1"
                     /translation="MSKKAWTIGFIALLAVIVWVFWFVEYRGEAGRLSALPEKAMHVV
                     FLDVGYGDCAWIRTPNGENFLIDAGPGTVGRLHDFAQTLYYSTANVKISLGEYLASQG
                     VRRLRAVFVSHSHFDHWGGLNALLSEGKISIGAVYESTSSLSTPAYDKFKTLLSEKGV
                     PVIGLFAGQDVAVGDPAVSLKVLGPRHAYLDTGYDLANRSLILRLTMGDVSVLFPGDI
                     GEEAEFDVLEVGELLRSTVLKISTHASRHATSTPFLDGVDPSIAVISLDTPNPFGTPH
                     DDMLQKLIDRNIEYYRTDVDGDVTFVTDGKKWRVSAAKRAG"
     gene            3837..4424
                     /locus_tag="P5079_07770"
     CDS             3837..4424
                     /locus_tag="P5079_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29918.1"
                     /translation="MNRPRSWCWGLYGFMVFWALGFSFLVWRHLQIEARRVFVEQHKT
                     LLHHAAVNLSDVLASEELLLAQASVRRLAGDPGARYAVLSDRSGRPAAADGALPSAEQ
                     EKILVGRMAAGAEARVYEVAAVPAFFEFSESVWVRGERWGALRWGVGAEDLSSTSRRQ
                     AFRIFLTWMWVVLVGAAPVFFAARRLERGDGGESA"
     gene            4421..5272
                     /locus_tag="P5079_07775"
     CDS             4421..5272
                     /locus_tag="P5079_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29919.1"
                     /translation="MKRWILPAALFAFLVFFVERVEGNARSQEVSLEGGSSGELYDLM
                     DKARSHAEQGRWFWACEAWQEALKNFPDADTARRRLFEALGTAEKKMADRGLPARERF
                     YFETLRCYLLLDNASLWGRWQRLEKDPRRAEEARWFLWGPSADFYRECAAAVGWKEAA
                     ELYGRGWKEYQDKRWRAAAAALRESVRTRSDHFLAAQCLALCEARLSKDEGPGLPAPL
                     WAGERYAGPLAGLVSAAENAFREGRWSLALAWMKNAPPSSLEAGVRERVRRAARRLEW
                     MFASRRP"
     gene            5377..>7257
                     /locus_tag="P5079_07780"
     CDS             5377..>7257
                     /locus_tag="P5079_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY29920.1"
                     /translation="MATPSAVGRKQVKDILTEAKLVSADDLNKAIEEAKKQDRPLQQM
                     VVDMKLADKVDVLQALSKEWRNKAVDLAEMEIDPDVVKIIPEATARRHHSLPFAKEDS
                     VLLVAMADPRDFFVSEDIHLRTGFEVQGYLAMPEDILRELDKVYGVAGANKASELMKG
                     VTEKATEAAGEGGEMKLEKFEEKTDVTEVDASAPEVEKIVNAIILAALQQKASDIHVE
                     PFEDPAGKNSRLLVRYRVDGFLREAGFQVPWSFRNAVMAKIKIMTSSMNITERRIPQS
                     GRIQVMAKGSPIEFRVEIVPTVYGESCVMRILDRKAVQVDIHRLGFLPDTLDRFLGLL
                     KGVGGKKNFGLCLVCGPTGSGKSTTLYAALNHINRPDIKILTAENPVEYNLDGIVQVQ
                     VNPDLKLGEDKCFDFATALRSFLRLDPDVIMVGEIRDQETAHIAMEAAMTGHLVFSTI
                     HTNDSPSSISRITDMGVPAFMVATTMKCVLAQRLCRRICKDCKTPHAPTLDEIAAFKA
                     NNLEIPAGAQLYSGKGCDTCGGSGFKGRCGIHELLVVDDVVRTATLKDVNADNIRNAA
                     MLESPQKMRIITQDGLMKALKLSDIQAKAIHELQLQRLTQLERLKIDEEYLELIKKIE
                     YYK"
BASE COUNT         1243 a         2111 c         2333 g         1570 t
ORIGIN      
        1 tgcccttcct gccggcgtgg ccggtttttt ctcggggggc ttcttattat tggataggcg
       61 ggctttcctt tgtttttatt ttcgcggctt ttttcgcgtt gtggcggctg aagtgggttc
      121 ccggtatcgc tttgcttctt ctgggtgggt tcagtttggg ggcttcccgc tattttcaga
      181 ccaccgacct caccaaccct catcacattt cccatttggc aggcgattcc tacgacaagg
      241 cggtgatcct ccgcgggacc atcatccagg accccgacta tcgggagcgc tacgtcaacg
      301 tcacccttca gccggaggag gtggtgttgg atccgaagaa accggaggag cgtatccgtc
      361 ttttcgggaa caccgggctt gtccgcgcaa cgatctatcc caatgtgggg aactattatt
      421 ggcgtgtttc ctacggcgac cgggtggaac ttgcgatgcc gctgatgaaa acgcgggacc
      481 ttaccaaccc gtcgggtttt aatttcggcc gctatctgcg cgcccgggga gtttacgcca
      541 ccgcgcctcc gtggggcatt cgtcagccgg gccacatccg ctatctgggg cgcggccgcg
      601 tcagctttct ggtgaagctg tctctcgatt tgaaactcaa ccttttaagt tccatccgtc
      661 gcaccatgcc gtatccggaa tcggcatttc tgggaggggt gacgctgggc ctgcgggccg
      721 ggttgccgca gaaaataaaa ctccagttcc aggagaccgg ggtggggcac gtgctggcgg
      781 tgtccggact gcacgtgggg ttcgtgcatg cgttgttgtt gatgatctgc cgggtttttc
      841 gtatccatcg gaggatttcc tatttcgtca tcgtcttcgg cctgatcgtg ttcgccatca
      901 tcacgggcgc ttcgccggcc acgcagcggg cggtattgat gtcctccatc ggccaggcca
      961 tctatacgtt cggcgggctg gggatccgcg gctccacggt gatgaccatt cctctggcgg
     1021 gaatgatcct gctgttgttg aacccgacca tcgcgccgga cggcagtttc gtgttgtcca
     1081 tcgtggcggt gtggtctctg gcgcatctgt cgggggccat ggtgcggttg ctggagatat
     1141 tcggccgggg gtggaaattt ctggcggggc tttttttcat gttggtgtgg accggcatcg
     1201 cggtgctcga cgccgccgcc tttacccggg cgccggcctg gatatccctt ggccttgtct
     1261 cggcggcggc cgtggccgcg gctttttatc ttgaccggaa atatcccatg cccggagtca
     1321 gccttttcgt cctgccgggt tggttcacgg gtctcgccgc cgcccagggg gccatacttc
     1381 tggggatgac gttcccgctt tcggcgatgt tttttcaacg ctactccgtt tcggggacgt
     1441 acgccaactt catcgccatc ccgctcatcg ggttcatcgt gcagttggga cttttggcgg
     1501 gattgctgga actcattttc acatcggtgg gcctcccggc ggtggggacg tacatcgccc
     1561 tctgcatcaa cgcttccaat tggtatctct gccagctctt tttgctggtg accgctttct
     1621 tctcgaagca tttcccttac ccctatgttc cgatgccttc cgtgagccaa cgcgtcattt
     1681 tctatgtcct tgtgatgggg ttcgttttct ggaaaccgtt gacgcagtgg gctcagtcgc
     1741 ttttggagtg ggcccgcacc ccgcgccgcc gatgggcggt tgtcggcgtt tttacgctac
     1801 tgatcggttc cattgcggcg cggaccaccg ccttttccga aatgcacgga aaaatgcggg
     1861 tgctgttctt cgattgcggc cacggcaatt ccatcctcat ccgcacgccg cacgggaagg
     1921 cgatcctcat cgacggcggg ccttcgggcc ggggcggatg gaacacgggg gagaccgtcc
     1981 tggctcctgt gttggcgaag ttcaacgtga agaacctgga aacggtggtg ttgacctcgc
     2041 cgcgcgaaag tcagggcggc gggctgccgt tcgtcctcga tcatttcacc gtcaataaga
     2101 ttttttcaac gctggacgga cggtatttct ctccccgcat gacgttcggc cagttcctcc
     2161 gcgcgttggg ggaccgctac ctcatcaaca accggcacga taaggagtcc catgaggctt
     2221 tcaccatgat gcacgatctt tttgtgatgg atgtttttcg tgcgaagatg gagaaggcgt
     2281 cggccggaga tgtcctctac gaggaagaag cgggcggcaa aaagctgatt ctgagggccc
     2341 tgtggccttt ggcggcgccg ttgcgcaaca ccaaggacga cgtggggaac aactccatgg
     2401 tgttgaagtt gacctacggt gaggtctctt tcctgtttgt gactcagctt cggcaggaag
     2461 gggagtgggc cttgctggac gccgccgggg acgaattgaa gtcggactgg ctgttggtcc
     2521 ccgccaacgg gaacattgag gcgtcttcgc cggagttcct ggaaaaggtc gccccgaaga
     2581 acgccgtgat gcaaacgggc tacgtgggct ggcgcgaccg ccggcggtat catgtggcca
     2641 ccaaacagtt ggacgccctg gtggaacgct atacgcggcg gggcatcact ctttaccgca
     2701 ccgatgtttc cggggcggtg accgtcacca ccgacggaga ggattcccaa agcgaaacgg
     2761 tcctccccgt cctcccgacg atttccgccc ccctgtcccc atcggacagg gaggcgaggg
     2821 ataaggatct ggagatattc ctttgatgtc gaagaaagcc tggacgatcg ggttcatcgc
     2881 gctgttggcg gtgatcgttt gggttttttg gtttgtcgaa tatcggggtg aggcggggcg
     2941 cttgtccgcc ttgcccgaaa aagcgatgca cgtggtgttc ctggacgtgg gatacgggga
     3001 ttgcgcctgg atacgcacgc cgaacgggga gaatttcctc attgacgccg gcccggggac
     3061 cgtcggacgg ttgcacgact tcgcccagac gctgtattac tcgacggcga acgtgaagat
     3121 ttctttgggg gagtatctgg cctcccaggg cgtgcgtcgt ctccgggcgg tgttcgtcag
     3181 tcattctcat ttcgaccatt ggggaggact gaacgcgctc ctttcggaag ggaagatatc
     3241 gatcggcgcc gtctatgaat ccacgtcgtc gctgtccacg cccgcttacg acaaattcaa
     3301 gacgctgttg tcggaaaagg gggtgccggt gatcggcctt tttgccgggc aggacgtggc
     3361 ggtcggtgat cccgccgttt ccctgaaggt cctgggcccc cggcacgcgt atttggacac
     3421 ggggtatgat ctggcgaacc ggtccttgat cctgagattg acgatggggg atgtctccgt
     3481 tctgttcccc ggcgacatcg gagaggaggc ggagttcgac gtgctggaag tcggcgagct
     3541 cttgcgttcc acggtgttga aaatttccac ccacgccagc cggcatgcca ccagcacgcc
     3601 ctttttggat ggcgtcgacc cttccatcgc cgttatttca ctggatactc ccaacccctt
     3661 cgggaccccc cacgatgaca tgctgcagaa actgatcgat cggaacatcg aatattaccg
     3721 cacggatgtt gacggggacg tcacctttgt caccgacgga aagaaatggc gcgtttccgc
     3781 cgccaagcgg gccgggtgag gtcccgccct cgtctgtcgt ctccccgcgt tctgctatga
     3841 accgtccccg ttcctggtgt tggggcctgt atggtttcat ggtgttctgg gccctcggct
     3901 tttccttcct tgtgtggaga cacctgcaaa tcgaggcgcg ccgtgttttc gtggaacaac
     3961 acaagaccct cctccatcac gccgccgtga acctgtccga cgttttggct tccgaggaac
     4021 ttcttctggc gcaggcttct gtccgtcgct tggcggggga cccgggggcg cgttacgccg
     4081 ttttgtccga ccgttccggc cggccggccg cggccgacgg ggctctgccc tcggcggagc
     4141 aggaaaaaat ccttgtgggc cggatggcgg ccggggccga ggcgcgcgtt tacgaggtgg
     4201 ccgccgtccc ggcgttcttc gaattttccg agagtgtttg ggttcgcggg gagcgatggg
     4261 gggctttgcg gtggggggtg ggcgccgagg atctgtcgtc gaccagccgc cgtcaggcgt
     4321 ttcggatatt tttgacgtgg atgtgggtgg tcctcgtcgg cgccgcgccg gtgtttttcg
     4381 cggcgcgtcg gcttgaaagg ggcgatgggg gagagagcgc gtgaaacgtt ggattctgcc
     4441 cgccgcgctg ttcgcatttc ttgtgttttt cgtcgaaagg gtggagggga atgcccgctc
     4501 tcaggaggtc tcgttggagg gggggtcctc gggggaactg tacgatctga tggacaaagc
     4561 ccgctcccac gcggaacagg gccgttggtt ttgggcgtgc gaggcttggc aggaggcgtt
     4621 gaaaaatttt ccggacgcgg acaccgcccg tcgccggttg tttgaggcgc tgggaacggc
     4681 ggagaagaaa atggccgacc gcgggcttcc cgcccgggag cggttttatt tcgaaacgct
     4741 ccggtgttac ctgcttctgg acaacgcctc gttgtggggg cgctggcagc ggttggaaaa
     4801 agaccctcga cgggcggagg aggcccgttg gttcctgtgg gggccgtcgg cggatttcta
     4861 tcgggaatgc gcggccgccg tcggctggaa agaggcggcg gaactctacg gccggggttg
     4921 gaaggagtat caggacaagc gctggcgggc cgccgccgcg gcgttgaggg aatccgttcg
     4981 gacgcgctcg gatcatttcc tggcggccca atgtttggcc ctttgcgagg cgcgcctctc
     5041 aaaggacgag ggtccgggcc ttcccgcgcc gctttgggcg ggggaacggt acgcgggccc
     5101 gctggccggg ctcgtctcgg cggcggagaa cgcgttccga gaagggcgat ggtccctggc
     5161 gttggcgtgg atgaagaacg cgcccccgtc gagcctggaa gcgggcgtcc gggagcgggt
     5221 gcggcgggcc gcccggcggc tggagtggat gttcgcttcc cgccggccct gagaaaggtc
     5281 tggaagttcc gccgttctcg tgttagaata caagaagtgc gcgcgccggg ctgcggcgcc
     5341 gccccaatag gaacactctg aggagggtcg aaggaaatgg ctaccccgtc cgccgtcggt
     5401 cgaaaacagg tcaaggatat ccttacggag gcgaagctcg tttccgccga tgacctgaac
     5461 aaggccatcg aagaggcgaa gaaacaggac cgtccgctcc agcagatggt cgtggacatg
     5521 aaactggcgg ataaagtgga cgttctccag gcgctctcca aggaatggcg caacaaggcg
     5581 gtggacctcg ccgagatgga gatcgacccc gacgtggtca agatcattcc ggaagcgacc
     5641 gcccgccggc atcactccct gccgttcgcc aaggaggatt ccgttctcct ggtggccatg
     5701 gccgaccccc gcgacttttt cgtttccgaa gacattcacc tgcgcactgg tttcgaggtg
     5761 cagggctacc tcgccatgcc ggaggacatc ctccgcgagc tcgacaaggt gtacggcgtg
     5821 gccggggcca acaaggcttc cgagctcatg aagggcgtca ccgaaaaggc caccgaggcc
     5881 gccggcgagg gcggcgagat gaagctggag aagttcgagg aaaagaccga cgtcaccgag
     5941 gtggacgcct ccgcccccga ggtggagaag atcgtcaacg ccatcatcct cgccgccctg
     6001 caacagaagg cctccgatat ccacgtggaa ccctttgaag accccgccgg aaaaaattcc
     6061 cggctgctcg tccgttatcg cgtcgacggt ttcctccgcg aggccgggtt ccaggtcccc
     6121 tggagtttcc gcaacgccgt gatggcgaag atcaagatca tgacctcgtc catgaacatc
     6181 accgaacgcc gcattccgca gtccggccgc atccaggtga tggccaaagg cagtcccatc
     6241 gagttccggg tggagatcgt gccgacggtc tacggcgagt cctgcgtcat gcgtatcctc
     6301 gaccgcaagg ccgtgcaggt ggacatccac cggctggggt tcctccccga tacgctggac
     6361 cgtttcttgg ggctgttgaa aggcgtgggc ggaaaaaaga atttcggact ctgtctcgtc
     6421 tgcggcccga ccggttccgg caaatccacc acgttgtacg ccgcgctcaa ccacatcaac
     6481 cggccggaca tcaagatcct taccgccgaa aaccccgtgg aatacaacct cgacggcatc
     6541 gtgcaggtgc aggtgaaccc ggacctgaaa ttgggagaag acaagtgttt cgacttcgcc
     6601 accgcgctcc gctccttcct gcgcttggat ccggacgtga tcatggtggg cgaaatccgc
     6661 gaccaggaga ccgcccatat cgccatggag gccgccatga ccggtcactt ggtgttctcc
     6721 accatccata ccaacgattc tccctcctcc atctcccgta tcacggacat gggcgtgccg
     6781 gctttcatgg tggccaccac gatgaaatgc gttctggcgc agcggttgtg ccggcgcatt
     6841 tgcaaagatt gcaagacgcc gcatgccccc acgctcgacg agatcgcggc gttcaaggcc
     6901 aacaacctgg agatcccggc gggggcccag ctctattccg gcaaggggtg cgatacctgc
     6961 ggcggctcgg gttttaaagg ccgctgcggc atccatgagc tgctcgtggt ggacgacgtg
     7021 gtccgcacgg ccacgttgaa agacgtgaac gcggacaaca ttcgcaacgc ggcgatgctg
     7081 gagtcccctc agaaaatgag gatcatcacc caggacggcc tgatgaaggc cctcaagctc
     7141 agcgacatcc aggcgaaagc catccacgaa ctacagctcc agcgccttac gcagcttgag
     7201 cgtctgaaga tcgacgagga atatctcgaa cttatcaaga agatcgaata ttacaaa
//
LOCUS       DAOTFD010000128         7177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7426_length_7177_cov_3.587475, whole genome shotgun sequence.
ACCESSION   DAOTFD010000128 DAOTFD010000000
VERSION     DAOTFD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7426_length_7177_cov_3.587475"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="P5079_07785"
     CDS             <1..415
                     /locus_tag="P5079_07785"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY29921.1"
                     /translation="GVGKKLVGKILGRLEEEGFQLMALRMVRPTQALMEEFYAEHKGK
                     PFFPPFLRFVTSGPLVVTAWAGENVVSRVRQIIGATNSPEAASGTLRRAWGTDNRRNL
                     VHASDSPASAARETAFFFTPQDLAPYDPDNWTKVG"
     gene            437..862
                     /locus_tag="P5079_07790"
     CDS             437..862
                     /locus_tag="P5079_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29922.1"
                     /translation="MSGDKRIVLIADDEPDSLELLKLLLERQNHDVITATNGREAIER
                     VIQFQPDLVLLDIMMPEIDGLAVCRHIKENDLLKRIKVILFSAKEKEKAQGDTTAKHV
                     GADAFLPKPLDPELLASVTAKMLAQQDREGPKLANKLSL"
     gene            923..2173
                     /locus_tag="P5079_07795"
     CDS             923..2173
                     /locus_tag="P5079_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY29923.1"
                     /translation="MSASRKPVELQFWVMPNAGFETRHILRHLLDRFHKTHPNIRVTP
                     EVFPWSVAWDRLMNVIKDGRGPLCPDVFQIGSTWTCTLAFLGALRELTPRLGEIDRDN
                     FIPTVFESCYLPHTRKLYALPWFIDLRVLYYRRDVLRAAGLGPEALETWDSFRRSCRL
                     LSKKYRRKNLYTLALSLQKGEVLIHDLAPWIWGAGGSFLTADEKHASFHREDSLEGLR
                     CFFSLMEDGSIPLFGRDRFSSGNFFSGHAAFQFSGAWPLNSAFKKGHPLYQPEVAENF
                     GAVPFPLGPAGRFTFLGGSHLAVSAMSRHPEESWSLVRFLSTPEAQLNHARAVGTLPC
                     RASEIPELFGEYPDIGRVFLESLTYARILPQIPTLGTVERVFSRCAMDVVKMIVDKGC
                     TPAKLQNRMADAALETNYILSLYG"
     gene            2166..3698
                     /locus_tag="P5079_07800"
     CDS             2166..3698
                     /locus_tag="P5079_07800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY29924.1"
                     /translation="MADLPSKTGRRSRAEFDFDTSDFCDFPVTHWRSVLPELHGVFER
                     AFFSLRRHSSFDMGAVFLFDPEAQLFRTAKIISGGEMLDGEEEMPLPPKSPMWRLIRR
                     ETDFLVLGPPDNAAFLPLAAFGTFLGALRVDRGRARKPFTRTERDFLVRFADALSTAM
                     WRVNYAVHDRLRDNQLRTFSEVSVLIHQSLRLKKLLEDVARKIVKNLGMDRVKIFLVD
                     RKRKSLQGELGYTLSEGLVDITDEKYPLKADGNPLVDALVGNPRNPMLTAFRQPVRYI
                     PLRARNEVMGVFMVDNLLSQQEISQDELRLLETLAGQLGLAVKNAELYEGVEELSITD
                     ELTRLYLPRYFKERLEQECQRSARADGSFAICMLDVDRFKNINDTYGHPIGDRVLQEL
                     AKQLRAASRQIDVVCRYGGDEFIILLPNTKAEQALQFAQRLRLRVRDVQIPLPHEQAL
                     QVGVSIGIAVYPEHSRQAEELIRLADTALYGAKSGGRNDARLYSPDARRQAPEINDVQ
                     PS"
     gene            3710..5152
                     /locus_tag="P5079_07805"
     CDS             3710..5152
                     /locus_tag="P5079_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29925.1"
                     /translation="MPDLSGRPRTDQRPSRWSKSILALSGGLAAVVLVEMGLRLGGFV
                     YQMKPERFNADALSRGGDVRVLCLGESTTQGAYPEHLQALLDNSGTPLKFVVIDKGIS
                     GTNSGIILMFLENLLDTYRPDLVLTMIGINDVGWSVPLKDGAGRIDRWFHRFRNLRIS
                     KLYRIATGERAGGASRRNVVVPKRTHDEFVPMSPITEKAPRTTQTSMTDYLNSGELFQ
                     QEGAWAEAEACFRNALKIDPKNELPHRFLGHLYERQRKYRKAIACFKNILELKTQGDP
                     AYIHRCLGSLYDKEKDYGNALVHYKKAVEINPTDEFSQVALVRFLYNRNARKEAYAGL
                     RKARDLNPTSLLLNSEFLKMKQYARPEKPKRETALSPYRAVTIRNYTKIRDIVLSRGA
                     RLVCVQYPMRPLRPLEEIIGKKQNVFYVDNELSFREAVRRDGYDIYFTDKFGGDFGHC
                     TVRGNRLLAENIVRVILHEVYPGRPSGTKP"
     gene            5165..5935
                     /locus_tag="P5079_07810"
     CDS             5165..5935
                     /locus_tag="P5079_07810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRY29926.1"
                     /translation="MGLIQTVKNIAKKSSALRKIHLKLHVSRQILSATGPRWELIRLF
                     HTVRPYTMVSNESLRNVYELAWDVERRGLAGAFVECGVWKGGCSAVMAYVRQKAGSRR
                     PQWLFDSFEGLPEPIPADGAKAEEYSGNRTSGRMLPVGQLVASIDDVREVFFKRLKLD
                     EAGVRIRKGWFQDTLPAAREEVGNIALLRLDGDWYESTRVCLENLYDRVVSGGYVVID
                     DYGHWEGCQKAVDEFFKARDIRVDLKTVDYSARYFKKP"
     gene            6287..>7177
                     /gene="rho"
                     /locus_tag="P5079_07815"
     CDS             6287..>7177
                     /gene="rho"
                     /locus_tag="P5079_07815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY29927.1"
                     /translation="MNAATLSKSTMADLVKLATEMKLASVSGLRKQELIAKILEAQGR
                     NSGVIIDQGVLEVLQDGFGFLRSPDYNYLPGPDDIYISPSQIKKFGLRKGDTVSGQVR
                     PPKDQERFFALLQVHSINGTSVEKVMTRSLFDNLTPLYPQKRLVLETKKDEINMRVLD
                     LVAPIGKGQRGLIVAAPRTGKTIILQKIANAISANHPEVYLIVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPADRHVQVAEMVIEKAKRQVEHGTDVVILLDSITRLARAYNTVTP
                     SSGRVLSGGLDSNALQRPKRF"
BASE COUNT         1511 a         2252 c         2078 g         1336 t
ORIGIN      
        1 cggcgtgggg aaaaagctcg tcggaaagat cctcggccgc ctggaagagg aaggttttca
       61 gctgatggcc ctgcggatgg tgcgtccgac gcaggcgctg atggaggagt tctacgccga
      121 acacaaaggt aaaccgttct tcccgccctt cctgcgcttc gtgacctccg gcccgctggt
      181 ggtcacggcc tgggcggggg aaaacgtggt gagccgcgtg cggcagatca tcggcgccac
      241 caattccccg gaggccgcct ccggcacgct ccgacgggcg tggggcacgg acaaccgccg
      301 caacctggtg cacgcctccg attccccggc cagcgcggcc cgggaaacgg cgttcttctt
      361 taccccgcaa gatttggcgc cctacgaccc tgacaactgg acgaaagtcg gttaattcag
      421 aaaacgaggt catcgcatga gcggagacaa acgcatcgtc cttatcgccg acgacgaacc
      481 ggactccctg gaacttctca agctccttct ggaacggcag aaccacgacg tcatcaccgc
      541 caccaacggc cgcgaagcca tcgaacgcgt catccagttc cagccggacc tggtcctttt
      601 ggacatcatg atgccggaga tcgacgggtt ggccgtgtgc cggcacatca aagaaaacga
      661 tttgctgaaa cgcatcaagg tcatcctctt cagcgccaag gaaaaagaaa aagcccaggg
      721 ggacacgacc gccaaacacg tgggcgccga cgctttcctc cccaaaccgc tcgatcccga
      781 actgctcgcg tccgtcaccg ccaagatgct ggcccagcag gaccgcgaag gtcccaaatt
      841 ggccaacaag ctcagcttgt agacggcgac ccctcttccg gcccgccctg aatccggcgc
      901 ccccggccgg tcccaaaaaa acatgagcgc ttcccgaaaa cccgtcgaac tccagttttg
      961 ggtgatgccc aacgcggggt tcgaaacccg ccacatcctg cggcatctgt tggatcgctt
     1021 tcataaaacc cacccgaaca tccgcgtgac gccggaggtg ttcccctggt cggtggcgtg
     1081 ggaccgcttg atgaacgtca ttaaggacgg tcgtggtccg ctctgtccgg acgtcttcca
     1141 gatcggcagc acctggacat gcaccctggc cttcctcggc gccctgcgcg agctgacccc
     1201 gcggttgggg gagatcgacc gggacaattt catccccacg gtctttgagt cctgctatct
     1261 gccgcacacc cgcaaactct acgccctccc ctggttcatc gacctgcgcg tgctttatta
     1321 ccgccgggac gttctgcgcg ccgccggctt ggggccggaa gcgttggaga cctgggactc
     1381 gtttcggcgc agctgccggc tcctttccaa aaaataccgt cgaaaaaacc tctatacgtt
     1441 ggccctctcc ctgcagaagg gggaggtgct catccacgac ctggccccct ggatctgggg
     1501 cgccggggga agtttcctca ccgccgacga aaaacacgcc tcgttccatc gcgaggactc
     1561 cctcgagggg ctgcgctgct tcttctcgct gatggaggac gggagcatcc cgttgttcgg
     1621 ccgggaccgg ttctcttccg gcaatttttt cagcggccac gcggcgttcc aattctccgg
     1681 cgcctggccg ttgaacagcg ccttcaaaaa aggccatccc ctgtaccaac ccgaggtggc
     1741 ggagaatttc ggcgccgtgc cttttccgct cggcccggcc ggccgcttca cattcctggg
     1801 agggagccat ttggctgttt ccgccatgag ccgccatccg gaagagagct ggtccctggt
     1861 gcgttttctc agcactccgg aagcccagtt gaaccacgcc cgggccgtgg gcacgctccc
     1921 ctgccgcgcg tcggagatcc cggaactctt cggggaatac ccggacatcg ggcgggtttt
     1981 ccttgaatcc ctcacctacg cgcgcatcct gccgcagatc cccacgctgg gcacggtgga
     2041 acgggtcttc agccgttgcg ccatggacgt ggtcaaaatg atcgtcgata aggggtgcac
     2101 gccggccaag ctgcagaacc ggatggccga cgccgccctg gaaaccaatt acatcctgtc
     2161 cctctatggc tgaccttccc tccaaaaccg gccgccggtc ccgtgcggaa ttcgacttcg
     2221 acaccagcga cttctgcgac ttccccgtca cgcattggcg gagcgtcctg ccggagctgc
     2281 acggggtgtt cgaacgggcc tttttcagtc tccgccgcca ttcgtcgttc gacatgggcg
     2341 cggtgttcct tttcgacccg gaagcccaac tcttccgcac ggccaagatc atcagcgggg
     2401 gggagatgct ggacggagaa gaggagatgc ccctgccgcc gaagtccccc atgtggcggc
     2461 tcatccgccg tgaaacggat ttcctggtcc tcggcccgcc ggacaacgcg gcctttttgc
     2521 ctctggcggc gttcgggacc ttcctcggcg ccctgcgggt ggaccgcggg cgggcccgga
     2581 aaccgttcac ccggacggag agggatttcc tggtccggtt cgccgacgcc ctttccaccg
     2641 cgatgtggcg cgtcaactac gccgtgcacg accgcctccg ggacaaccag ctccgcacct
     2701 tcagcgaggt gagcgtcctc atccaccaat cgctccgcct taaaaaactc ctggaagacg
     2761 tggcccgcaa gatcgtcaaa aacctcggca tggaccgggt gaagatattc ctggtggacc
     2821 ggaaacggaa gtccctgcag ggcgaactcg gctacaccct ctcggaaggc ctggtggaca
     2881 tcaccgacga gaaatacccg ctgaaagccg acgggaatcc cctggtggac gccctcgtcg
     2941 gcaatccgcg caatcccatg ctgaccgctt tccgccaacc cgtccgctac atccccctgc
     3001 gcgcccgcaa cgaggtcatg ggcgtgttca tggtggacaa cctcctcagc cagcaggaga
     3061 tctcccagga cgagctacgg ctcctggaga cgctcgccgg acaactggga ctggccgtga
     3121 agaacgccga actctacgaa ggggtggagg aactctccat caccgacgaa ctgacgcggc
     3181 tctatctgcc gcgctacttc aaggaacgac tggagcagga atgccagcgg tcggcgcgcg
     3241 ccgacggttc gttcgcgatc tgcatgctgg acgtggaccg attcaagaac atcaacgaca
     3301 cctacggcca ccccatcggg gaccgcgtgc tccaggaact ggccaaacag ctccgggccg
     3361 cctcccgcca gatcgacgtg gtgtgccgtt acggcgggga cgagttcatc atcctgttgc
     3421 cgaacaccaa ggcggaacag gccctgcagt tcgcccaacg ccttcgcctg agagtgcggg
     3481 acgtgcagat ccctctgccg cacgaacagg cgctgcaggt gggggtgagc atcggcatcg
     3541 cggtgtatcc ggaacattcc cgccaggccg aggagctcat ccggctggcc gataccgcgc
     3601 tttacggggc caagtcgggc ggacgtaacg atgcccggct ttattcgccc gacgctcggc
     3661 gccaggcccc cgaaatcaac gacgttcaac cgtcgtaacg cccccgacca tgccggatct
     3721 cagcggacgc ccccgaacgg atcagcgccc ctcccggtgg tcgaaatcca ttcttgcgct
     3781 gtcgggcgga ctggcggcgg tcgttctggt cgaaatgggg cttcgcctcg ggggtttcgt
     3841 gtatcaaatg aaacccgaac gattcaacgc ggacgccctt tcacggggag gagacgtacg
     3901 cgtcttgtgt ctaggggaat cgacgaccca gggggcctat ccggaacatc tgcaagctct
     3961 gctggataac agtggaacgc ctctgaaatt tgtcgttatc gataaaggaa tcagcggcac
     4021 gaattcgggg atcatcctca tgtttttgga aaatcttttg gacacgtaca ggcccgattt
     4081 ggtccttact atgatcggta tcaatgacgt cggttggtcc gtcccgttga aggacggcgc
     4141 gggaagaatc gatagatggt tccaccggtt tcggaatctc cgaatttcca aattgtatcg
     4201 aatcgccaca ggagaaagag cggggggggc gagccggcga aatgtcgtgg ttcccaaaag
     4261 gacgcacgat gagtttgtcc ccatgtcccc gatcaccgag aaggccccga ggacgacaca
     4321 aacatcgatg accgactatt taaattcggg ggaactgttc cagcaggagg gggcctgggc
     4381 cgaggcggaa gcttgtttca ggaatgctct gaagatcgat ccaaaaaacg aattgcccca
     4441 tcgttttctt ggccatcttt atgaaagaca gcgtaaatac cgaaaagcaa tcgcgtgttt
     4501 caagaatatc ctggagttaa agactcaggg agatccggcc tatatccacc gatgcctcgg
     4561 ctcgctgtat gacaaagaga aagattacgg caacgctctt gtccactaca aaaaagccgt
     4621 agaaatcaac ccgaccgatg aattctccca agtggcgctc gtccgattcc tctacaaccg
     4681 aaacgcccgc aaagaagcat acgctggtct cagaaaagcg cgggatctga accccacgag
     4741 tcttttgctg aactctgagt tcctcaaaat gaaacaatat gcgcggccgg aaaaacccaa
     4801 gagggagacg gctctgtctc cctatcgcgc cgtcacgatt cgaaactata ccaagatacg
     4861 cgacatcgtc ctgagtcgag gagctcgtct tgtgtgcgtt caatatccga tgagaccgct
     4921 gcgaccgctg gaagaaatca tcgggaaaaa acagaatgtt ttttatgtgg acaatgagct
     4981 cagttttaga gaggccgtcc ggagagacgg ttatgacata tactttacag acaagttcgg
     5041 cggagatttc ggtcattgca ctgtccgagg gaaccgtctg ctggccgaaa acatcgtccg
     5101 ggtcattctc cacgaagtgt atccaggccg tccgagcggc acgaaaccgt aaaggagata
     5161 ttccatgggt cttatccaaa ccgtcaaaaa tatcgcgaag aagagcagcg ccctccggaa
     5221 aatccacctg aagctgcatg tctcccggca gatcctgtcg gccaccggcc cgcgctggga
     5281 actgatacgg cttttccata cggtccggcc gtatacgatg gtcagcaacg agagtctccg
     5341 taacgtgtat gagctggcgt gggacgtgga acgccgcgga ctggccgggg cttttgtgga
     5401 gtgcggggtg tggaaaggcg gctgttcggc cgtgatggcc tacgtgcggc agaaggccgg
     5461 ttcccggcgg ccccagtggc tcttcgattc gttcgaggga ctgcccgaac ccatccccgc
     5521 ggacggggcg aaagcggagg aatattccgg aaaccggacg tctggaagga tgctccccgt
     5581 gggacagctc gtggcttcca tcgacgacgt ccgggaggtt tttttcaagc ggctcaagct
     5641 ggacgaggcg ggtgtgcgca tccgaaaagg atggttccag gacacgctcc cggccgcccg
     5701 cgaagaggtg ggaaacatcg ccctcctccg cctcgacggc gattggtatg aatcgacccg
     5761 ggtgtgtctc gagaacctct acgacagggt ggtgtccggg gggtacgtcg tcatcgacga
     5821 ctacggccac tgggagggct gtcagaaagc cgtggatgaa tttttcaaag cgcgggatat
     5881 ccgggtcgac ctgaaaacgg tggattactc cgcccggtat ttcaaaaaac cgtaaagccg
     5941 ctcccggggc ccgggacgcc acaaaaaatc ttgacacccg aatcgccttt agtataataa
     6001 gggtgaaatc gcccaccagc gacgcttctt cagcggatcc ccgaaaccgg ttttttctca
     6061 aatacattgt ctgtcgggac tttacctctg ctccccgcgg cgcgctcggt gaatctgaaa
     6121 aatccctgca tcctggaagt tcgttccctg tcgcattttc ccttttgagt cggtagcgct
     6181 ttaaaaaaat ccttacctaa tagccatcac caccccaacg ctggaggaat tcacaatgga
     6241 gcaacaggta caacaaccca ccgcacaacc ccacaacggg ccggaaatga acgccgccac
     6301 gttgagtaaa tccaccatgg cggaccttgt caaactggcc acggagatga agctggcgtc
     6361 cgtttccggc ctcaggaaac aggagctgat cgccaagatt ttggaggccc agggccgcaa
     6421 ctccggcgtc atcatcgacc agggcgtgct ggaggtcctc caggacgggt tcgggttcct
     6481 ccgcagcccg gattacaact atctgccggg tccggacgac atttacattt ctccctccca
     6541 gatcaagaag ttcggcctgc gcaaaggaga cacggtctcc ggccaggtgc gaccgcccaa
     6601 ggaccaggag cgttttttcg ccctgctcca agtgcattcc atcaacggca ccagcgtgga
     6661 gaaggtcatg acgcggtcgc ttttcgacaa cctgaccccg ctgtatccgc agaaacgcct
     6721 cgtgctggag acgaagaagg acgagatcaa catgcgggtc ctcgacctgg tggcgcccat
     6781 cggaaagggg caacgcggac tgatcgtggc cgccccgcgc accggcaaaa ccatcatcct
     6841 tcagaaaatc gccaacgcca tctccgccaa ccatccggaa gtctatctga tcgtcctcct
     6901 catcgacgaa cgccccgaag aggtcacgga catgcagcgg agcgtgaagg gggaagtgat
     6961 ctcctccacg ttcgacgagc cggccgaccg gcacgtgcag gtggccgaga tggtgatcga
     7021 gaaggccaaa cggcaggtgg aacacggcac ggacgtggtg atcctgctgg actccatcac
     7081 ccgtctggcg cgcgcctaca acacggtgac gccctccagc ggccgggttc tctccggcgg
     7141 actggattcc aacgccctgc agcggccgaa acgcttt
//
LOCUS       DAOTFD010000129         7172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7438_length_7172_cov_5.177884, whole genome shotgun sequence.
ACCESSION   DAOTFD010000129 DAOTFD010000000
VERSION     DAOTFD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7438_length_7172_cov_5.177884"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="P5079_07820"
     CDS             <1..445
                     /locus_tag="P5079_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY29928.1"
                     /translation="DEPTAGVSPEARARFWELIRDVAGAGKTVFVTTHYMDEAEQCGR
                     IALMRAGRIIALDTPAGLKRSAFPEPLLELTPPAAVSPGWADRLRKNTAVALLVPYGM
                     RFHAALRPPADWSALAGDLPKGTTHRRIAPSLEDVFLRLVEGGTG"
     gene            445..1563
                     /locus_tag="P5079_07825"
     CDS             445..1563
                     /locus_tag="P5079_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29929.1"
                     /translation="MDAFRWDRALSIARKEVRHIRRDPFTLAAGLGIPVLLVLFFGFV
                     MDFDVRDIRLLVQDADRSRASRELLELFRASGHFDARSADPVADPAALLAREKAKAVL
                     VIPPGFGRDRDRGVPARAQVLLDGADNSTAGVILSYVAGIQQAAARRGAGADAAEPVE
                     VRTRFLFNPELNSRWFVVPGLAAVVIGLLAVLLTALTVAREWENGSMELLLSTPVRPL
                     EILVGKLAPYVALGMGGVLLVYLTARFVFDVPFEGSHALFFSASFLFLGAALGQGLLI
                     SVTTRQQPLAMQLGIVTGLLPSLLLSGFVFPIESMPLFFRALTALLPVRWYMTVTRGI
                     FLRGAGPADLWVPLTVLTAMSLALSALALKKFKTDLEP"
     gene            1560..2660
                     /locus_tag="P5079_07830"
     CDS             1560..2660
                     /locus_tag="P5079_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY29930.1"
                     /translation="MNPTLVAFVRKELLQSLRDVRMRAVIFVLPVVQLTLFGLALSTE
                     VRNIRLATDFSPLDVLSRRVAEACLATGWFVPAARGAGDPFHWVRAGGADAGMVSPRG
                     GLARAVERGPATLQLLVDATNTIRARSIEVYFKTVLGGVLRETSPGPARASPVLLEPR
                     VLYNPSMRTAVFLVPGVLSMILCVVTVILTSMSMAREKEMGTFETLVSAPLSNTEILL
                     GKTLPYVLLGVADAVIVLTVAVFGFRVPMQGPVWQVLLAAVVFVFSTVAAGTLISTVA
                     RNQQQAMMGSFLFLFPAMLLSGIMFPLENMPRALGWIAYLNPLKYFVVLMRNIMLKGG
                     DAWVVGGNLLELAALGMVLSWLAARRFHQTLN"
     gene            2677..3006
                     /locus_tag="P5079_07835"
     CDS             2677..3006
                     /locus_tag="P5079_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY29931.1"
                     /translation="MANVNEIRDRGFELAGVLEYQEGAVVSRTLLERKTGTVTVFAFD
                     EGQGLTEHTSLSEAMVYVLDGEADISLAGKTHRLKAGEMLLMPANVPHALRAVKKFKM
                     LLILLRP"
     gene            complement(3013..3963)
                     /locus_tag="P5079_07840"
     CDS             complement(3013..3963)
                     /locus_tag="P5079_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29932.1"
                     /translation="MNKVFGPVPSRRLGRSLGINNIPPKICSYNCVYCQVGRTSDLSP
                     ERKPFYPPEELFAEVRTRLKTLRDAGEPVDALTFAPDGEPTLDENFGRAVDLLKTLGV
                     KVAVLTNASLLFREDVRNELARADWVCLKVDTVDEKLWRRVNRPAPAVTLSSLLDGMR
                     AFSKIYRGELVTDSMLLAGYNDDPPGVRKLAGFLRELKPKKAYLSVPTRPPAEQSVKT
                     PSTAAVENACRLFQEALGPAAECPGHEEDSPLAYTGDVERDVLDMAAVHPVREDQLRG
                     YLERAGKDWDLIVRLLDAGQLLEKTHDGKKFYLKKLPRVS"
     gene            complement(4102..5535)
                     /locus_tag="P5079_07845"
     CDS             complement(4102..5535)
                     /locus_tag="P5079_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY29933.1"
                     /translation="MDVLLVNTNRMKPPIAPLAFDYLGGTLKTAGHRARLLDFGLAED
                     PAQAARRAFAGSRPDVVALTFRNSDDCYFASGASFVDVLRNDVELLRQHHDGPLLVGG
                     GGFSVLPELLLDHAGADYGVVGDGETALLEFLRFRRGDIAAEAVPGLVHRRRGVWRRN
                     PVRPADLAAPGLSARDTVDNAAYFRRGGQAGVETKRGCPGRCVYCADPPIKGRSVRRR
                     RPDDVVSEVRNLLAQGIDHLHFCDSEFNLPADHAEDVCRALIDGGVAPKIRWFAYAAP
                     QPFTPDFAALLRRAGCAGVNFGADSASDPMLKRLGRDHSADRLAETARLCRKNKITFM
                     YDLLIGGPGETPDTARETLRAVQKMEVDRVGVAVGVRVYPGTPLAEQIKREPALRAGL
                     RGDPESLAPAFYVSPALGADPFDLVRTLTGNDDRFLLPSGKDEKDYNYNDNTVLARAV
                     EAGHRGAYWDILRRLKQGLPPGAVGRD"
     gene            5920..6669
                     /locus_tag="P5079_07850"
     CDS             5920..6669
                     /locus_tag="P5079_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29934.1"
                     /translation="MSIELEKILRPVDHPMEPFPPKFMTLADGSEMVVRQVGREDMPS
                     LLPHVAQLMLVERDQYDIVASRVYAELLSYYTHRVQDEYVLVAQIDGELAAIVNGRLV
                     NPEVGMSYHTLAFRRGLRVGAHAFAAKMEYHIDILKQKEVLIVAESPIGFRRWMIEYK
                     LEKRFHIPHELGGVPSWALTKELFDAARGSLIVGRRPVPGKLLKKAQEAILPPSDPPK
                     PSKFLMAATRAKNDPTGTSLMLQRWKQEGRG"
     gene            6671..>7172
                     /locus_tag="P5079_07855"
     CDS             6671..>7172
                     /locus_tag="P5079_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRY29935.1"
                     /translation="MRNVYVAGLGLTAFSRLEFPLVELAGYPAMQAMKDAGVDRVDHL
                     YVANMGGSRLNRQTGLASAVVDSLSLTPAGAESIENGPASGASALKQGYMAVASGLHD
                     VVMVVGAERMREVNNLEATDFVATLTHPLAEYIYGVTLPSLAGMFTRLYMEKYGVTSR
                     HLAMVAV"
BASE COUNT         1074 a         2200 c         2580 g         1318 t
ORIGIN      
        1 ggacgaaccc accgccgggg tgtctccgga ggcgcgggcg cggttctggg aactgatccg
       61 cgacgtggcc ggcgccggca agaccgtttt cgtgaccacc cattatatgg atgaggcgga
      121 gcagtgcggc cgcatcgcgc tcatgcgcgc gggccgcatc atcgcgctgg atacaccggc
      181 cggactgaag agatcggcgt tccccgaacc cctgctggaa ttgaccccgc cggccgctgt
      241 ttctcccggc tgggccgacc ggctgcggaa aaacaccgcc gtggcgctgt tggtgccgta
      301 cggcatgcgg ttccacgcgg cgctccgtcc gccggccgac tggtccgccc tcgccggcga
      361 cctgccgaaa ggcaccacgc atcgccgcat cgccccgtcg ctggaggacg tttttctgcg
      421 gctggtggaa ggagggacgg gctgatggac gccttccgtt gggaccgggc gttgtccatc
      481 gcccgcaaag aggtgcggca catccggcgc gacccgttca ccctggcggc cgggctcggc
      541 attccggtgt tgttggtgct gttcttcggg ttcgtgatgg atttcgacgt gcgggacatc
      601 cggttgttgg tgcaggacgc cgaccggagc cgggcttcgc gggaactcct cgaacttttc
      661 cgggcttccg gccatttcga cgcgcggtcg gcggacccgg tggccgaccc ggccgccctg
      721 ctcgcccggg agaaggccaa ggcggtgctg gtgattccgc cggggttcgg ccgggacagg
      781 gaccgcggcg ttccggcgcg ggcgcaggtg ctcctggacg gcgccgacaa ctccaccgcc
      841 ggcgtgattt tgtcgtatgt ggcgggcatc cagcaggccg ccgcccgccg cggggccggc
      901 gcggacgcgg ccgaacccgt ggaggtgcgg acgcggttcc ttttcaaccc ggaactgaac
      961 agccgctggt tcgtggtgcc ggggttggcg gcggtggtca tcggcctgtt ggcggtgttg
     1021 ctgaccgcgc tgaccgtggc gcgggaatgg gagaacgggt ccatggaact gctgttgtcc
     1081 acgccggtgc ggccgctgga gatcctggtc ggcaaactgg cgccttacgt ggcgctgggc
     1141 atgggcgggg tgctgttggt gtatctcacc gcgcgttttg tgttcgacgt gccgttcgag
     1201 ggaagccacg cgctcttctt ctccgcgtcg ttcctttttc tcggcgcggc gctggggcag
     1261 ggactgctca tctccgtcac cacgcggcag cagccgctgg ccatgcagtt gggcatcgtc
     1321 accggcctgt tgccgtcgct gcttctgtcg gggttcgtgt tccccatcga gagcatgccg
     1381 ctctttttcc gcgcgctgac cgccttgctg cccgtgcggt ggtatatgac ggtgacgcgg
     1441 gggatcttcc tgcgcggcgc cgggccggcg gacctgtggg ttccgctgac ggtgttgacg
     1501 gcgatgagcc tggcgctgtc ggcgctggcg ctcaaaaaat tcaagacgga cctggaacca
     1561 tgaaccccac gctcgtcgct ttcgtgcgca aggaactgct gcaaagcctt cgggacgtgc
     1621 gcatgcgggc cgtgatcttc gttttgccgg tggtacagtt gaccctcttc ggactggcgc
     1681 tctccacgga agtgcggaac atccggctgg cgacggactt ttcgccgttg gacgtccttt
     1741 cccgccgggt ggcggaggcg tgtctggcca cgggctggtt cgtgccggcg gcgcggggcg
     1801 ccggcgatcc gttccattgg gtgcgcgccg gcggcgccga cgcggggatg gtgtctccgc
     1861 gcggcgggct ggcgcgggcg gtggaacgcg gccccgcgac cctgcaactg ttggtggacg
     1921 ccaccaacac tattcgggcg cgcagcatcg aggtttattt caagaccgtc ctcgggggcg
     1981 ttctgcgtga gacgtcgccc gggccggccc gggcgagccc ggttttgctg gaaccgcgcg
     2041 tgttgtacaa cccgtccatg cgcaccgcgg tcttcctggt gcccggcgtg ctttccatga
     2101 tcctctgcgt ggtgacggtg atactgacca gcatgtccat ggcgcgcgaa aaggagatgg
     2161 gcacgttcga gacgctggtg tcggcgccgc tttcgaacac cgagatcctt ctggggaaga
     2221 cgctgcctta cgtgctgttg ggcgtggcgg acgcggtgat cgtgctgacg gtggcggtgt
     2281 tcgggttccg cgtgcccatg cagggacccg tgtggcaggt gttgctggcg gcggtggtgt
     2341 tcgtgttcag cacggtggcc gccggcacgc tcatctccac ggtggcccgg aaccagcagc
     2401 aggccatgat ggggagtttc cttttcctgt ttcccgccat gctgttgtcc ggcatcatgt
     2461 tcccgctgga gaacatgccg cgggcgctgg gctggatcgc gtacctcaac ccactgaagt
     2521 acttcgtcgt gctgatgcgc aacatcatgt tgaagggcgg ggacgcctgg gtggtgggcg
     2581 gcaacctgct ggaactggcg gcgctgggga tggtcctctc ctggcttgcc gccagacggt
     2641 tccaccagac gttgaactga aaaaaggaga ggcgctatgg cgaacgtgaa tgagatacgg
     2701 gaccgcggct tcgagttggc cggggtgttg gagtatcaag aaggggcggt ggtgagccgc
     2761 accctgttgg agcggaagac gggcacggtg acggtgttcg ctttcgacga gggacagggg
     2821 ttgaccgaac acacctcgct gtcggaggcg atggtgtacg tgctggacgg agaggcggac
     2881 atctcgctgg ccgggaagac gcaccgcctg aaggccgggg agatgctgct gatgcccgcc
     2941 aacgtgccgc acgcgctgcg cgcggtgaag aagttcaaga tgcttctgat cctgttgcgg
     3001 ccgtgagggg cctcaggaga cgcgggggag ttttttcagg tagaactttt ttccgtcgtg
     3061 cgttttttcg aggagttgtc cggcgtcgag gagccggacg atgaggtccc agtcttttcc
     3121 cgcccgttcc agatagccgc gcaactggtc ttcccgcacc gggtggaccg cggccatgtc
     3181 gagcacgtca cgttccacgt cgccggtgta ggcgagcggg gaatcctcct cgtggccggg
     3241 acactccgcc gccgggccga gcgcctcctg gaagaggcgg caggcgtttt cgaccgcggc
     3301 cgtggagggg gtcttcacgg attgttcggc cggcggccgc gtggggacgg agaggtaggc
     3361 cttcttcggt ttcagttcgc gcaggaaccc cgccagtttg cgcacgcccg gtgggtcgtc
     3421 gttgtacccg gcgaggagca tgctgtccgt gaccagttcc cctcggtaga tcttcgagaa
     3481 cgcccgcatt ccgtccaaaa gggacgataa cgtgaccgcc ggcgccggcc ggttgacccg
     3541 ccgccagagt ttttcatcca ccgtgtccac tttcagacag acccagtccg cccgggccag
     3601 ctcgttccgg acgtcttccc ggaaaaggag ggaggcgttg gtgagcaccg ccactttgac
     3661 gccgagcgtc tttaaaaggt ccacggcgcg gccgaaattt tcgtccaggg tcggttcgcc
     3721 gtcgggagcg aaggtgaggg cgtccaccgg ttcgccggcg tcgcgcaggg tcttgagccg
     3781 ggtccttacc tccgcgaaga gttcttcggg cggatagaac ggtttccgtt ccggcgaaag
     3841 gtcgctggtg cggcccacct ggcagtagac gcagttgtag gagcagattt tcggcgggat
     3901 gttgttgatg cccagactcc tccccagccg ccgcgacggc accggtccga acaccttgtt
     3961 cattgttttc cgtcccttcg atgacgttct ctcggaatca ttttactttg acgggccgaa
     4021 gatgtccaac ccctcgatcg gtcgaacgtg gccggggcgc ctcccgcgcc gttggcgcgg
     4081 acgggcggtc cggagggagg gtcagtcgcg gccgacggcg ccggggggga ggccctgttt
     4141 gaggcggcgg aggatgtccc aataggcgcc gcggtgtccg gcttcgacgg cccgggcgag
     4201 gacggtgttg tcgttgtagt tgtagtcctt ttcgtctttg ccggacggaa ggaggaaccg
     4261 gtcgtcgttg ccggtgagcg tgcgcaccag gtcgaagggg tccgcgccga gggcggggga
     4321 gacatagaac gccggggcga ggctctccgg atctccgcgg aggcccgccc gaagggccgg
     4381 ttcgcgtttg atctgttcgg ccagcggggt gcccggatag acgcgcacgc ccacggccac
     4441 tcccacgcgg tccacctcca tcttctgaac ggcgcggagg gtctctcggg cggtgtccgg
     4501 ggtttcgccc ggtccgccga tgaggaggtc gtacatgaag gtgattttgt tctttcggca
     4561 gagccgggcg gtctcggcga ggcggtcggc ggagtggtcc cgtccgagcc gtttcagcat
     4621 ggggtcggag gcgctgtccg cgccgaagtt gacgccggcg cagccggcgc gccgcaggag
     4681 cgcggcgaaa tccggggtga acggttgcgg ggcggcgtag gcgaaccagc ggatcttcgg
     4741 ggccacgccg ccgtcgatga gggcgcggca gacgtcttcg gcgtggtccg ccggcaggtt
     4801 gaactcgctg tcgcagaaat ggagatggtc gatgccctgc gccagcaggt tccgaacctc
     4861 ggagaccacg tcgtcgggcc ggcgccggcg gacggagcgt cctttgatgg gcggatcggc
     4921 gcagtacacg caccgaccgg gacaaccgcg cttggtctcc acgccggcct ggccgccccg
     4981 gcggaaatac gccgcgttgt ccacggtgtc ccgcgcggag agccccgggg cggcgaggtc
     5041 cgcgggccgg acggggttgc gcctccagac gccgcggcgg cggtgaacca ggccgggcac
     5101 cgcctcggcc gcgatgtcgc cccgtcggaa gcggaggaat tcgaggaggg cggtctcgcc
     5161 gtcccccacc acgccgtagt ccgccccggc atggtccagc aggagttccg gcagcacgga
     5221 gaaaccgccg ccgccgacga gcagggggcc gtcgtggtgt tggcggagga gttcgacgtc
     5281 gtttcgaagg acgtcgacga acgacgcccc ggaggcgaaa tagcagtcgt cgctgttgcg
     5341 gaaggtgagg gcgaccacgt ccggccggct ccccgcaaac gcccgccgcg cggcctgggc
     5401 ggggtcctcg gcgaggccga agtcgagcag gcgcgcgcgg tgccccgcgg ttttcagcgt
     5461 gccgcccaga taatcgaacg ccaacggcgc gatgggcggt ttcatgcggt tggtgttgac
     5521 gagaaggacg tccatgggtt tattttggca gattttttcg aaaaccgccc gggattatcg
     5581 cgcatggaga aaatcggccg cgccggctaa aggccgttgc gggttttaaa gggtccgtct
     5641 ttgttttccg acgaaacagt ccttccgcca tcgcgaggcc tgtttggggg ccgtggccct
     5701 ttcgcaggag ctcaaggacc gaatccggcc agaggccgaa ggagcgatct cttccttcac
     5761 tcaacagggg attgcttcgt cgaaaaaacg cgactcgcaa tgacaggcgc tgtgaaatct
     5821 aggacaggtt ctggggagag cccttgtccg gggggcgatt tgggtgtatg cttgagtacc
     5881 gtttttttcc acgtcccctc atcttccagg aggcttccga tgtcgatcga acttgagaaa
     5941 atactgcgtc cggtggacca ccccatggaa ccgttccctc ccaaattcat gacgttggcc
     6001 gacgggagcg agatggtggt gcgccaggtg gggcgggagg acatgcccag tctgctgccg
     6061 cacgtggccc agctgatgct ggtggaacgg gaccaatacg acatcgtggc cagccgggtg
     6121 tacgcggaac tcctgtccta ttacacccac cgggtgcagg acgaatacgt gctggtggcg
     6181 cagatcgacg gcgaactggc cgcgatcgtc aacggccgcc tggtgaaccc cgaggtgggg
     6241 atgtcctacc ataccctggc ttttcgccgc ggactgcggg tgggggcgca cgccttcgcc
     6301 gccaagatgg agtaccacat cgacatcctc aaacaaaaag aggtgttgat cgtcgcggag
     6361 tcgcccatcg ggttccggcg gtggatgatc gaatataaac tggagaagcg gttccatatc
     6421 ccgcacgaat tgggcggggt cccctcctgg gcgctcacca aagagctgtt cgacgccgcc
     6481 cggggcagcc tcatcgtcgg ccgccgtccg gtgccgggaa aactcctgaa gaaggcgcag
     6541 gaggcgattt tgcctccctc cgatccgccg aaaccgtcga aattcctgat ggccgccacg
     6601 cgcgccaaga acgaccccac cggcacctcc ctcatgctgc agcgctggaa acaggaaggg
     6661 cggggctgat atgcgcaacg tctatgtggc cggcctcggc ctgacggcgt tcagccggct
     6721 ggagttcccg ctcgtcgagc tcgccgggta cccggcgatg caggcgatga aggacgccgg
     6781 ggtggaccgg gtggaccatc tgtacgtggc caacatgggc ggcagccggc tgaaccgcca
     6841 gacgggactg gccagcgcgg tggtggacag cctcagcctg acgcccgccg gggcggaatc
     6901 catcgagaac gggccggcgt cgggggcgtc ggccctcaag cagggctaca tggcggtggc
     6961 ctccggcctg cacgacgtgg tgatggtcgt cggcgcggaa cggatgcgcg aggtgaacaa
     7021 cctggaggcc acggacttcg tggccacctt gacccatccg ctggcggaat acatctacgg
     7081 cgtcacgctg ccgtccctgg ccggaatgtt cacccggctg tacatggaaa aatacggggt
     7141 gacctcccgc cacctggcca tggtggcggt ga
//
LOCUS       DAOTFD010000130         7131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7503_length_7131_cov_5.164924, whole genome shotgun sequence.
ACCESSION   DAOTFD010000130 DAOTFD010000000
VERSION     DAOTFD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_7131_cov_5.164924"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="P5079_07860"
     CDS             <1..1131
                     /locus_tag="P5079_07860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY29936.1"
                     /translation="DAKPELHKTPFRAVFIVGQKMADLHLRNLSLYSPHTPFVLFFPN
                     VRHLKQIRLEKHTLNRLRKKNILNDLYTLASRILVTTRDDADTIQKEVSLFLHSDVLP
                     SAGDGPADWLKGFVRAMPPRREAHVTKKLVSIIILCHNDLHYLKQCVDSIKKYTHVPY
                     ELIFVDNASEDDSQRYLRSIKGAKVIINPVNLAFAKGNNQGAQIANGEYILLLNADVV
                     VTEGWLDRLLRCAESDPSIGLVGPCTNSATKVQTVVAPYKSIKDLPRFAMAHSLKNDS
                     VWEDVQRLIAFCLLIKREVIQKVGLLDERFGPGGYEDYDYCLRVNQAGYKIKLCKDVY
                     IHHFGGKGYSGMDYDRLRDVNREIFIEKWCRKSLEFLELIRY"
     gene            1165..2313
                     /locus_tag="P5079_07865"
     CDS             1165..2313
                     /locus_tag="P5079_07865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRY29937.1"
                     /translation="MTEAASLLPSFKTDCRHFRPDRPCAPHKSRGVVCRDCPVYDPVS
                     RRVLLLKTGAVGDVLRTTALLPALKEKHPNAFVTWVAHPAAFPLFDGSPHLDRLLPFS
                     WETLGILRRERFDLLLSLDFDPAVAAWVPEIRADEKRGFVYSDGLLTALNEEARGWVD
                     MSLNDRLKRANRRTYPDWMFSLCGFSRAERYEPQWTVSPEEKSLASRFFSKLRSAGRK
                     KIVGLNTGAGRRWQQKKWPEKNWIEFIRLLQKEKDILPVLLGGPEERGRNRLLKKKFP
                     RLLDTGCDNSLRGFGALVNHCDAVVTGDTLAMHIAIALKKRVIALFGPTSSAEIELYG
                     RGEKLHRDWDCLPCYLPRCTRSPHCMEALSPKTIADAVRRQIGLLPSR"
     gene            2310..4280
                     /locus_tag="P5079_07870"
     CDS             2310..4280
                     /locus_tag="P5079_07870"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29938.1"
                     /translation="MKMPQFPADRSLPLRAAAWLALAAGFFLYLPTISFEFLWDDTYF
                     IVQNPFLREPEHLWTVFSHFVSKHVLPVPGGARPMWVSSLVWDYSLWGLNPAGFHFTN
                     VLLHAMNCALAVLLAHRILGGARPALAVGVAFAAHPLHVEPVAAVSFRTDLLCLLFLQ
                     LACLCYWPKDAGEPQAPRGRSLRRFLPYPASLLFFIAALLSKETAAALPLLLTTGDYL
                     RYPARTALKRLALVLPFVFLVAAFYAHHKGRFDYKLSDAVQSVASLTGETLDHVLLRP
                     AAAWAEEPRPPAAPPPSTEGLVYRAPLVNACTMGNVFARYVVKLVFPLSLSADYTVAI
                     RTSPASPVVAGSLLLFFGGLALFFIGRRRDPALSWGLAWFYIFLIPVSNVVRIVNLMT
                     DRYLYIPSLGFLVFGAALFRQAERRADTWPRYGRTLKRLLWAAALLLLAFWTFQTLLY
                     SQAWRDEVSFYTKTLESSPANFRPWYNAGLAFQRRDRWEKALVYYRQAAERAPRRKEI
                     YVNMGRCYEALGDTDAAETEYRHALRVDPGDAAALLNLGNLQRQRGRLPEAADYYRRA
                     MDAAPRYSLAAAHLAETYRAMNQLDKAAEICGHLLDWEPANGRAYQLLGEIYIAQGDR
                     ARAKDLWRLFLKKNPDSPLRNHFDRLIRETHS"
     gene            4277..5761
                     /gene="tilS"
                     /locus_tag="P5079_07875"
     CDS             4277..5761
                     /gene="tilS"
                     /locus_tag="P5079_07875"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRY29939.1"
                     /translation="MTPLLPHKKSRETAVDLYPEFRAALGALLPSRKSSLLIACSGGP
                     DSMALWDLAERWEEETGGRVVLGHINHGLRGRASDADERFVRAESSRRHTPFFSAAVP
                     VKAFARREGRSLEDAARVLRYRALARLARAAGCPVVAAAHTLDDQAETVFLHLLRGTG
                     PLGLAGMPPSSPWPVPNDDTTPEHESLRRPRLLRPLLSISKSRLRAYLKSRRIPFRVD
                     ASNDSPVFLRNRIRPILKKWEALRPGLRRRIARLSEIQRDEEAHWTSRWRREFPSLFR
                     RKNAALRLEPARFKRYDRCEQRRLLRAAFRRLDFDGLEKTRALALSPGPTGRVSVPGG
                     WIEKKGRALVFRPYTPKGPSATTAAPRPQRLSVPGRISFRDAWKARHWEIRAEPVKRL
                     PRDWRRDPLRVYVDADRLGATLFCRTWRPGDRFQPLGLAGRKKLQDFFVDEKLPRQTR
                     KETPLLENSNGIVWVVGHRIADAVKLTPRTRRLLKLEARRELSA"
     gene            5758..6375
                     /gene="hpt"
                     /locus_tag="P5079_07880"
     CDS             5758..6375
                     /gene="hpt"
                     /locus_tag="P5079_07880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRY29940.1"
                     /translation="MKSTLQDLGPRFHGWPKRDIERVLVSETAIKRCVRRLGEQISRD
                     YEGQKIILVSVLKGSVVFMADLLREINIPCAVDFMAVSSYVGSETSGVVRVTMDLRES
                     PEGKNIILVEDIVDTGLTLAYLKKNLLTRRVKSLRVCALLDKPDCHRVPVSLNYMGFS
                     IPNEFVVGYGLDYAERYRSLPHIAVLKREVYKPSSGEERSKSSGG"
     gene            6381..>7131
                     /locus_tag="P5079_07885"
     CDS             6381..>7131
                     /locus_tag="P5079_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRY29941.1"
                     /translation="MKGNGRNLLLWLLVFAGVLFFIQSARTARLGEEEIAYSKFKRFL
                     HEGRVMDVRVREDLIRGQYRGEDSKPHLFKTVPLNDPNLVTDLEQAGVERFSGEIDRG
                     WLNAFLVNALWIGLFFFLWWFIIIRQMQGGGKQALAFGRSRAKLQSNKKQKVTFNEVA
                     GCDEAKEELQEIIEFLKEPAKFQKLGGKIPKGVLLFGAPGTGKTLLAKAVAGEAGVPF
                     FSSSGSDFVEMFVGVGASRVRDLFEQGRKNAP"
BASE COUNT         1265 a         2561 c         1980 g         1325 t
ORIGIN      
        1 gacgccaaac cggaactcca caagacgccg ttccgcgccg tcttcatcgt cggccagaaa
       61 atggcggacc tccacttgcg caatctttcc ctctacagcc cccacacgcc gttcgtcctg
      121 ttttttccca acgtccgcca cctgaaacag atccgcctcg aaaaacacac gctgaaccgg
      181 ctgcgcaaaa agaacatcct gaacgacctc tacaccctcg ccagccgcat cctggtgacc
      241 acccgggacg acgccgacac catccaaaaa gaagtcagcc tgttcctgca ttccgatgtc
      301 ttgccctcgg ccggagacgg cccggcggac tggctgaagg ggttcgtccg ggccatgccg
      361 ccgcgccggg aagcgcacgt gacgaaaaaa ctcgtgagca tcatcatcct ctgccataac
      421 gacctccatt acctcaaaca gtgcgttgac agcatcaaga agtacacgca cgtgccctat
      481 gagctcatct tcgtcgacaa cgcctcggaa gacgactccc agcgatatct gcgctccatc
      541 aagggcgcca aggtcatcat caatccggtc aacctcgcct tcgccaaggg gaacaaccag
      601 ggcgcgcaga tcgccaacgg cgaatacatc ctcctgctca acgccgacgt ggtggtcacc
      661 gagggctggc tggaccgcct gctccgctgc gccgaaagcg acccgtccat cgggctcgtc
      721 ggcccctgca ccaacagcgc cacgaaggtg cagaccgtgg tggcccccta caagtcgata
      781 aaggacctgc cgcggttcgc catggcccac tccctcaaga acgattccgt ctgggaagac
      841 gtccaacgtc tcatcgcctt ctgcctcctc atcaaacggg aggtcatcca aaaggtcggt
      901 cttctggacg aacgttttgg accggggggg tatgaggact acgactactg cctccgagtg
      961 aaccaagcgg gctacaaaat aaaactctgc aaggacgtct acatccacca tttcggcgga
     1021 aaaggctatt ccggcatgga ttacgaccgg ctccgcgacg tcaaccggga aatcttcatc
     1081 gagaagtggt gccggaaatc gctggaattc ctggaactca tccgttacta gcccacctcc
     1141 tcccgcaacc gcgtcaggat cgccgtgacg gaagccgctt ctttgctccc ctctttcaag
     1201 accgattgcc gtcattttcg gcccgaccgg ccttgtgcgc cccacaaatc acggggggtc
     1261 gtctgccggg actgccccgt ctacgacccc gtttcccggc gggtcctcct cctgaaaacc
     1321 ggggccgtcg gcgacgtcct acgcaccacc gccctccttc cggccctgaa ggaaaagcat
     1381 cccaacgcgt tcgtgacctg ggtcgcgcac cccgcggcct tccctctctt cgacggctcg
     1441 ccccatctcg accgcctcct ccccttttcc tgggaaaccc tcgggatcct tcgccgggaa
     1501 cgttttgacc tgctgctgtc cctggacttc gatccggcgg tggccgcctg ggtcccggag
     1561 atccgcgccg acgaaaaacg cgggttcgtc tattccgacg gcctgttgac cgccctcaac
     1621 gaagaggccc gcggctgggt ggacatgagc ctcaacgacc gactcaagcg cgccaaccgg
     1681 cgaacctacc cggactggat gttctccctc tgtggatttt cccgggcgga acgatacgaa
     1741 ccgcaatgga ccgtctctcc cgaagaaaaa tccctggctt cccggttttt ctcgaaactc
     1801 cggtcggccg gccggaaaaa gatcgtcggc ctcaacaccg gcgccggccg acgatggcag
     1861 cagaagaaat ggccggaaaa gaactggatc gaattcatcc gcctcctcca gaaagagaaa
     1921 gacatcctcc cggtgctcct cggcggaccg gaggaacgcg gccgcaaccg gctcttgaag
     1981 aaaaagttcc cccggctgct cgataccggc tgcgacaact cccttcgggg tttcggcgcc
     2041 ctcgtgaacc actgcgacgc ggtggtcacc ggagacaccc tggccatgca catcgccatc
     2101 gccctgaaga aacgcgtcat cgccctgttc ggccccacct cctccgcgga gatcgaactt
     2161 tacgggcggg gcgaaaaact ccaccgggac tgggactgcc tcccctgcta cctcccccgg
     2221 tgcacccgct ccccccactg catggaggcg ctttccccaa aaaccatcgc cgacgccgtt
     2281 cgccgccaga tcgggctcct gccctctcga tgaaaatgcc gcagttcccc gcggacagat
     2341 cgctcccgct gcgcgccgcc gcgtggctgg ccttggccgc cgggtttttt ctctatctgc
     2401 cgacgatttc ctttgaattc ctctgggacg acacctattt catcgtccag aatccgttcc
     2461 tccgggaacc cgaacacctt tggaccgtct tctcccattt cgtctcgaag cacgtccttc
     2521 cggtgcccgg cggcgcgcgg ccgatgtggg tgagcagcct ggtctgggat tattccctct
     2581 gggggctgaa ccccgcgggg ttccatttca ccaacgtcct cctccacgcg atgaactgcg
     2641 ccctggcggt cctcctggcc caccggatcc tcggcggcgc acggccggcc ctggccgtcg
     2701 gcgtcgcctt tgcggcgcat cctctgcacg tggaacccgt ggccgcggtc agcttccgaa
     2761 ccgacctcct ttgccttctt ttcctccaac tggcttgtct ctgctattgg ccgaaagacg
     2821 ccggggagcc gcaggccccc cgcggtcgtt ctctccggcg ttttcttccc tatccggcct
     2881 cgctcctctt tttcatcgcc gcgctgttgt ccaaggaaac cgcggcggcc ttgcccctcc
     2941 tgctgacgac gggggattat ctccgctatc cggcaagaac cgccctgaaa cgcctcgcgc
     3001 tcgtcctacc tttcgtgttc cttgtcgccg ctttctatgc ccaccacaaa ggacggttcg
     3061 actacaaact ctccgacgcc gtccaatccg tcgcctcgct gaccggagaa accctggatc
     3121 atgtcctgct gcgcccggcg gcggcctggg cggaagagcc ccgcccgccc gccgccccgc
     3181 ccccgtccac cgaaggactt gtctaccgcg ccccgctcgt caacgcctgc accatgggaa
     3241 acgtcttcgc ccgttacgtc gtcaaactcg tcttccctct ttccctttcc gccgattaca
     3301 cggtggccat ccgcacctct cccgcctctc ccgtggttgc gggctcgctc ctcctctttt
     3361 tcggcggact ggctctcttc ttcatcggcc gccgccggga tccggccctc tcctgggggc
     3421 tggcctggtt ttatatcttc ctcattccgg tctccaacgt ggtccgcatc gtcaacctca
     3481 tgacagaccg ctacctttat atcccgtccc tcggtttcct ggtcttcggc gccgccctct
     3541 tccgtcaagc ggaacgccgc gcggacacct ggccccgtta cggccggaca ctgaaacggc
     3601 tcctgtgggc cgccgccctt ctccttttgg cgttctggac gttccagacg ttgctgtatt
     3661 cccaggcctg gcgcgacgaa gtttccttct acacgaaaac gttggaatcc tcgccggcca
     3721 atttccgccc ttggtacaac gccgggctgg ccttccagcg cagggatcgg tgggagaaag
     3781 ccctggtcta ctaccgccag gcggcggaac gggccccgcg gagaaaagaa atctatgtaa
     3841 acatgggccg ctgttacgag gccctcggcg acaccgacgc cgccgaaacc gaataccggc
     3901 acgccctgcg cgtggacccc ggggacgccg cggcgctctt gaacctcggc aacctccaac
     3961 gtcagcgcgg acgcctcccc gaggccgcgg actattatcg ccgcgccatg gacgccgccc
     4021 cccgctattc cctggcggcg gcccacctcg ccgaaaccta tcgggccatg aaccagctgg
     4081 acaaggccgc cgagatctgc ggacacctgc tcgactggga accggcgaac ggccgcgcct
     4141 accagctgct cggcgaaatc tatatcgctc aaggagaccg cgcccgcgca aaggacctgt
     4201 ggcgtctgtt tttgaaaaaa aatcccgact ctcccctgcg caaccatttc gatcgattga
     4261 ttcgagagac ccattcttga cccctcttct tccccataaa aaatcgcgcg agacggcggt
     4321 ggacctctac ccggagttcc gggcggccct gggcgccctt cttccctccc gaaaatcctc
     4381 tcttctgatc gcctgttccg gcgggccgga ctccatggcg ttgtgggatt tggcggagcg
     4441 ctgggaggag gaaaccgggg ggcgcgtcgt cctcggtcac atcaatcacg gactgcgggg
     4501 ccgcgcctcg gacgccgacg aacggttcgt ccgggccgag tccagtcgcc gacacacccc
     4561 ctttttttcc gcagccgtgc cggtcaaggc tttcgcccgg cgcgaaggcc gttccctgga
     4621 agacgccgcc cgcgtcctcc gttatcgcgc cttggcgcgt ctggcgcgcg ccgccggctg
     4681 tcccgtcgtg gccgccgccc acaccctgga cgaccaggcg gaaacggtgt tccttcacct
     4741 cctccgcggc accggccccc tcggtctggc cggcatgccg ccctcctctc cctggccggt
     4801 cccgaacgac gacacgacgc cggaacacga gtccctccga agaccccgcc tcctccgccc
     4861 gctcctctcc atttccaaat cgcggctccg agcctatctg aaatcccgcc gaatcccctt
     4921 ccgcgtcgac gcctccaacg attctcccgt cttcctgaga aaccgtatcc gaccgatcct
     4981 gaaaaaatgg gaagctctcc gccccggact tcggcggcgg atcgcccgcc tgtccgagat
     5041 acagcgggac gaggaggccc actggacgtc ccgctggcga cgcgaattcc cgtctctttt
     5101 ccgacggaaa aacgccgccc tccgccttga accggcccga tttaaaagat atgataggtg
     5161 cgaacagcgg cggctgctcc gcgccgcatt ccgccgcctg gacttcgacg gcctggaaaa
     5221 gacccgcgcc ctggcgttgt cgccgggacc caccggccgc gtttccgttc cgggcggatg
     5281 gattgaaaaa aaagggcgcg ccctcgtttt tcggccttac actccgaaag gtccctccgc
     5341 cacgacggcc gcgccgcggc cgcaacggct gtccgtcccg ggaaggattt cttttcggga
     5401 cgcctggaaa gcgcggcact gggagatccg ggccgaaccc gtcaaaaggc ttccgcgcga
     5461 ctggcgacgc gacccgctcc gcgtgtatgt ggacgcggac cggctgggcg caacgctctt
     5521 ctgtcggacc tggcgaccgg gggaccggtt ccagccgctg ggccttgccg gccggaaaaa
     5581 acttcaggat ttttttgtgg acgagaagct tcctcgtcaa acccgaaagg agacgccgct
     5641 cttggaaaac agcaacggca tcgtctgggt cgtgggccat cgcatcgccg acgccgtgaa
     5701 attgacgccc cgcacccgcc gcctcctcaa actcgaagcg cgccgggaac tttccgcgtg
     5761 aaatcgacgt tacaggattt gggaccccgg ttccacggct ggccgaaacg cgacatcgag
     5821 cgcgtcctcg tttccgaaac ggccatcaaa cgatgcgtcc gccggctggg cgaacagatc
     5881 tcccgcgact acgaggggca gaagatcatc ctggtctcgg tgctcaaggg gagcgtcgtc
     5941 ttcatggccg acctccttcg ggagatcaac atcccctgcg ccgtggattt catggccgtc
     6001 tcctcctacg tgggctcgga gacctccggc gtggtgcgcg tcaccatgga cctgcgcgag
     6061 agcccggagg ggaagaacat catcctcgtg gaagacatcg tggacaccgg cctcaccctg
     6121 gcctatttga aaaagaatct gctgacgcgt cgcgtgaagt ccctgcgcgt ctgcgccctg
     6181 ctggataagc ccgattgcca ccgcgtgccg gtgtcgctca actatatggg cttttccatc
     6241 cccaacgaat tcgtggtggg ctacggactg gattatgccg aacgctaccg cagtctgccg
     6301 cacatcgcgg tcctcaagcg ggaggtctac aaaccctcct ccggcgagga gcggtcaaaa
     6361 tcgtcaggag gctgaaacac atgaaaggca acggacggaa ccttctcctc tggctcctcg
     6421 tgttcgcggg agtcctcttc ttcattcaaa gcgcccgcac ggcgcggctg ggcgaggagg
     6481 agatcgccta ctccaaattc aaacgcttcc tgcacgaggg ccgcgtgatg gacgtccgcg
     6541 tccgcgaaga cctcatccgc ggccagtacc gcggcgaaga cagcaagccg cacctcttca
     6601 aaaccgtgcc gctgaacgat cccaacctgg tcaccgacct cgaacaggcc ggggtggagc
     6661 ggttctccgg agagatcgac cgcggctggc tcaacgcctt cctagtgaac gccctctgga
     6721 tcggcctttt cttcttcctc tggtggttca tcatcatccg gcagatgcag ggcggcggca
     6781 agcaggccct ggcgttcggc cggtcccgcg ccaaactgca gtcgaacaag aaacagaagg
     6841 tcacgttcaa cgaggtggcc ggctgcgacg aggccaagga ggaactgcag gagatcatcg
     6901 agttcctgaa ggagccggcc aagttccaga aactcggcgg caagatcccc aagggggtgc
     6961 tcctcttcgg cgcgcccggc accgggaaaa cgctcctggc caaggccgtg gccggggagg
     7021 ccggcgttcc gttcttctcc tcctcgggtt ccgatttcgt ggaaatgttc gtgggcgtgg
     7081 gcgcctcgcg cgtgcgcgac ctgttcgagc agggccgcaa gaacgccccc t
//
LOCUS       DAOTFD010000131         7131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7505_length_7131_cov_4.521905, whole genome shotgun sequence.
ACCESSION   DAOTFD010000131 DAOTFD010000000
VERSION     DAOTFD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7505_length_7131_cov_4.521905"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="P5079_07890"
     CDS             <1..1132
                     /locus_tag="P5079_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRY29942.1"
                     /translation="ARFYMTTWLNQDVEASFRLQSINLWGMEGNNTLVTRYPSADGGP
                     WVENVYIHMPNFAWKKVNLTVGRQPIAYGDGLIVSDDELGFNAIRAQMDFFRSRLDVD
                     MFTAKVTEGFGEDDFDLNGVMLGTNREHNRWELSWVQEQNNTAGAYVRPTTTVTANDV
                     TREFYDLRLFGDLKDAYYKLELALQRGNADLATNESIRLKGMGQKLELGAQTDTARWG
                     RFGVRAVYAVGSGDDAGTPSVDEAFRPTFARRWDGLQRTGYGTHFGGTLSDAYAGATP
                     FSPTATGLPLGFSGIKTMGLGIFSTQGVLWTGSVDYYTFNSLTKPTGLNDLGTEIDAS
                     IIYRYTGFVTFKLGAAYFFPGEVYGHTASRVTRYTAETHVHF"
     gene            complement(1170..1475)
                     /locus_tag="P5079_07895"
     CDS             complement(1170..1475)
                     /locus_tag="P5079_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY29943.1"
                     /translation="MSEIRWEAERIKKRRLELRYSQKQLAEKIGTTQGAVAQMETGIR
                     SPSATVLPRLAKALRVSVDYLLVGEAAEMLDVGGLDAGDRHLLRRFRDFLLWEKKGG"
     gene            complement(3168..3542)
                     /locus_tag="P5079_07900"
     CDS             complement(3168..3542)
                     /locus_tag="P5079_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29944.1"
                     /translation="MKKTAGIIVGLMLWGSSSLWADRLCDRCAQERQSRMVEKKIDRL
                     TQKLKLSGEQQDVIRRILRSEAEKKAELKKEASMRLDALHEETQTEIKKTLASEQQSR
                     FEAIWEQSQDKKAKGRRSCCHK"
     gene            complement(3695..4360)
                     /locus_tag="P5079_07905"
     CDS             complement(3695..4360)
                     /locus_tag="P5079_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29945.1"
                     /translation="MDDPKRILQRLSAGLCLLAAGCAGINVIEDKALEITPARNFSEN
                     VKSIKVRVWKLEKDAQAEYKFDKEVLETVERVLKGKGYGVEIVWDDPFSYGDRNDERF
                     QYRGYIHVLNDERFLSEDTAFLEIANTWEERLEDRVRTHTGDVEVGHLEDETGHRIAT
                     EYRPQTTTREYTVHHYKSSANASLFYGDRKAIYDRYASVQADNLLQVTEAVLREIPKR
                     KRE"
     gene            complement(4538..4614)
                     /locus_tag="P5079_07910"
     tRNA            complement(4538..4614)
                     /locus_tag="P5079_07910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4578..4580),aa:Met,seq:cat)
     gene            complement(4680..5291)
                     /locus_tag="P5079_07915"
     CDS             complement(4680..5291)
                     /locus_tag="P5079_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY29946.1"
                     /translation="MKKFLKSMFTGLALLGLAAPTHAKEPRIGQKAPNFTLQDVRGQK
                     RSLSDFKGQYVVLEWTNPDCPFVRKHYDSGNMPALQKEMTAKGVVWLAIASSAKGKQG
                     YYEPSAWEKILREKKSAPTALLRDPAGTVGRMYGAKTTPHMFVIDPEGLLLYKGAIDD
                     KPSTDPDDVATAKNYVRAALEEALSGKPVTTSDTSAYGCSVKY"
     gene            complement(5316..6170)
                     /locus_tag="P5079_07920"
     CDS             complement(5316..6170)
                     /locus_tag="P5079_07920"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRY29947.1"
                     /translation="MFLKKTLSRAVLFFSALGFSAAPAGTEGPHVRAEFFSDVRSVRP
                     GEKFWTAVRLRMDRGWHTYWKYPGDSGMPTRLRWTLPPGLTAGDVRWPCPIKFTASSL
                     VSYGYKDEVWLLVPLEAAAALPEGRVPLEVRVDWLECADICVPGRADLRLELPVQNAP
                     SDKDASLMKSFALALGRLPAELSPEKVTVTEGPRDVHVRLSLPEPPPGTPAPVFYPAT
                     AATAEKLSEPRIRRERDAVILRFAKKTPSEPVPAVAGVLVFVPEGEAPPRGFEFQKKL
                     EQAAPRRR"
     gene            complement(6177..6635)
                     /locus_tag="P5079_07925"
     CDS             complement(6177..6635)
                     /locus_tag="P5079_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29948.1"
                     /translation="MKKFRNALLLAAALALGLSFALRAADPVPDGAVKKSYPTGELMS
                     EYSYLYGKLDGLSLDYYKNGQVMYEWNYRRGKLHGVSRAYSMKGKLMASWSYKNGVLH
                     GKSVEYHDNGKKKSYVVYRQGQKVFIKTFNEKGKLLQKEDRRKLPAPTAG"
     assembly_gap    6837..6846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6919..>7131)
                     /locus_tag="P5079_07930"
     CDS             complement(6919..>7131)
                     /locus_tag="P5079_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRY29949.1"
                     /translation="SIAILSVQLLLDWLSVGASWNEDPWTYRINFPGTLGDHNWTLRL
                     PVSLEELQGLPVNGVIREINRASGRV"
BASE COUNT         1395 a         2277 c         1909 g         1540 t
ORIGIN      
        1 cgcccgcttt tacatgacca cctggctcaa ccaggacgtg gaagccagtt tccgcctgca
       61 gtccatcaac ctctggggca tggaaggcaa caacaccctc gtcacgcgct atccctcagc
      121 cgacggcggc ccttgggtcg agaacgtcta catccacatg cccaacttcg cctggaaaaa
      181 agttaacctc accgtcggcc gccagcccat cgcctacggc gacggcttga tcgtctccga
      241 tgacgaactc gggttcaacg ccattcgagc gcaaatggat ttctttcgca gccggttgga
      301 tgtggacatg ttcacggcca aggtcacgga gggattcggg gaggacgatt tcgacctcaa
      361 cggcgtcatg ctcggcacca accgcgagca caaccgctgg gaattgagct gggtgcagga
      421 acagaacaac accgccggcg cctacgtgcg ccccaccacc accgtcaccg ccaacgacgt
      481 cacgcgggaa ttctacgacc tccgcctctt cggcgattta aaagacgcct attataagtt
      541 ggaattggcc cttcaacgcg ggaacgccga tctggcgacc aacgaaagca tccggttgaa
      601 ggggatgggg cagaaactgg aattgggcgc ccaaacggac acggcccgct ggggccgctt
      661 cggcgtccgc gccgtctatg ccgtcggctc gggcgacgac gccggcaccc ccagcgtgga
      721 cgaggctttc cgtccgacct ttgcccggcg gtgggacggc ctgcaacgga ccgggtacgg
      781 cacccatttt ggcggcaccc tgtccgacgc ctacgccggc gcaacgcctt tttcgcccac
      841 cgccaccggt ttaccgctcg gattttcggg gatcaagacc atggggctgg gaatcttcag
      901 cacccaggga gtcctctgga ccggctcggt ggactactac accttcaact ccctcaccaa
      961 accgaccggg ttgaacgatc tgggaacgga aatagacgcc agcatcatct accgctacac
     1021 gggttttgtg accttcaaac tcggagccgc ctacttcttc cccggcgagg tctacggcca
     1081 cacggcctct cgggtcaccc gctacaccgc cgaaacccac gttcacttct aactgatttt
     1141 taccggtccg acggagaggg aaaacctccc tacccgccct tcttttccca cagcaaaaaa
     1201 tcgcggaatc gcctcagcag gtgccgatct cccgcatcca gcccgcccac atccaacatc
     1261 tccgcggcct cgcccaccaa aagataatcc accgaaaccc gaagcgcttt cgccaatcgc
     1321 ggcaggaccg tggccgaggg ggaacggatc cccgtctcca tctgggcgac cgccccctgg
     1381 gtggttccga tcttctcggc cagctgtttt tgactgtaac gcagctccag ccgcctcttt
     1441 tttatacgtt ccgcttccca tcgtatctcc gacacagccc acctccgccc cgggtgtatt
     1501 acattatgta attatatatt actaaatgta atacacctcc tccagcgggg gaggacaccg
     1561 aacggcccat gcccctcaaa ggccggcgaa atgcccgcca cgccgccact cgccgaacgt
     1621 caatcccaag accaagaccc aacgggccgt tcaccccctt cacgctccgc ctggaggggg
     1681 atattgcccg aacctgagcc gttccggaaa catcttgtca cattccattg cgcagacccc
     1741 tcccttgtcg cgcaaatgca gttccgaacg atccccagag acatcacccc cgaagcccct
     1801 ccaaggcctt aaagcaacaa aaacgccagc gcgacaaggt cgcaatttga gcctatcaac
     1861 acagaacctc ctccaagaga aggtctcgag aaacacctcc aaactcagac tccccgcgct
     1921 ctccgcatta ctcgaggacc ggcggcgaga tacggaaggg tccgcacaaa aaactcccgt
     1981 cgctctctca gcccaagaaa agacacgccc aatcattcgc cccaaagggc aagacgctcg
     2041 gaattcgcca aaatgacacc agaaaaacct ccacagccca ccccagccca gccccatatt
     2101 aacttccgat aatacatatt atgttaagtt acgacgggga agagaccgtc gccctttcat
     2161 ctatattttg tataagattt tatctttaaa tcgtttatta tcaacatttt taagtcatga
     2221 atttattctt tgtattagct aattattcct ggcactcgat ttttggcaaa aatttttgag
     2281 aatttcccac ccgcgccatt ttcaatgatt ttttacgatt tgaacctctc tcaaagttgc
     2341 caactttcat gcgactttaa aacaccataa aatgggttgc ggtgatccgt gcgtcttcca
     2401 gggtcaccca aggccttctc gcccttcctt ataaaagacc atgagacgac caccccgtcc
     2461 caacccccgt tttttgccaa ctttccaagg gtccaccctc tccccgaaaa gaccataaag
     2521 caccctcatc ccttccagcg gttccacccg cccggcccgc tcccaaaatc gcctcccttc
     2581 aaaaacacca cgagccgaca acccttgaaa tgccaacaaa atcttgattt caaaaaagac
     2641 cacgaagcgc tccggctcct ttcatcgact tttaaattcg cctctaaaaa tagagtcgaa
     2701 tttaagggta tcggaggccg tccaggcgat tttaggcccc taaaatcgca tttttgtgcg
     2761 ttttccgtcg agaaaaccct tcaaattcca gtttcaacct cctcccgttt cggaaaaagg
     2821 cgaagcgcgc cgccgcggtt ctggatccgg ggctgtcgca ctccagattt tttggaaaac
     2881 gcgtcccctg aaatccgatg gggcgctttt cttttggaaa tacccggacc cgccgccacg
     2941 ttgcgaacct ttggggccct gttttttatt ttgcgatcaa acaaattcca aaaccgtgga
     3001 atttcctggc gatggcgcgg cctcaaacac ccgacacgca cacaaaaaaa tgcccgccgc
     3061 gcgatgacgc cgccggcggg cataaaaaaa ccgactcccg gaaggaatcg gtggtctcaa
     3121 caacaggaat accgctgctg ttctacgaac ggggccgcag gacggatcta cttatgacag
     3181 caagaacggc ggcctttcgc ttttttgtcc tgggactgtt cccagatcgc ctcaaaccgg
     3241 gactgctgct ccgatgcaag cgtcttcttt atctccgtct gggtctcttc gtgcaacgcg
     3301 tcgagccgca tggaggcctc tttcttcagc tccgccttct tctccgcctc gctccgcaat
     3361 atccgccgga tgacatcttg ctgctcccca gacaacttca atttctgcgt caaccggtcg
     3421 attttcttct ccaccatccg ggattggcgt tcctgggcgc accggtcgca caatcggtcg
     3481 gcccagaggc tcgacgaccc ccacagcatc aatcccacga tgattccggc ggtcttcttc
     3541 acgttatgtc ctttcttcgg cttattggct gtcggttgat tcgaagggat gacggttccc
     3601 cgaggccctc cggagcgatc gttcccgaat cgaaatatct cggggttctc ggtccttcgc
     3661 ggtgttccgc gcctcccgag gaccctgtcg tcggctattc ccttttcctt ttcggtattt
     3721 cccgcaacac cgcctcagtg acctgcagca gattgtccgc ttgaaccgag gcgtaccggt
     3781 cgtagatggc tttcctgtcg ccatagaaca gcgacgcgtt ggcggacgac ttgtagtggt
     3841 ggaccgtgta ttcccgcgtg gtcgtttgcg gccggtattc cgtggcgatg cggtggcccg
     3901 tttcgtcctc gaggtggccg acctccacat cgccggtgtg ggttcttacg cggtcctcca
     3961 accgctcctc ccatgtgttg gcgatttcaa gaaacgccgt gtcttccgaa aggaatcgct
     4021 cgtcgttgag cacatggatg tagccgcggt attgaaacct ctcgtcattt cggtccccat
     4081 aggagaaggg gtcgtcccaa acaatctcca ccccatagcc cttccccttc agtacccgct
     4141 ccacggtctc caggacctcc ttgtcgaact tgtattcggc ctgagcgtcc ttctccagtt
     4201 tccacacccg aacctttatg gatttcacgt tttcggaaaa attccgggcg ggggttattt
     4261 caagggcctt gtcctcgatg acgttgatgc cggcgcaacc ggccgccagc aggcaaaggc
     4321 cggccgacag gcgctgaaga attcgcttgg ggtcatccat cttttcccct ccgttttata
     4381 tcccgcccga tgtgtcgatt ttccagcatg gtggtttgga tttccgatga gacagcccgc
     4441 aacggccgag gcccgctccc agccgacatc ccttgttcga gcggacacga acgaagcccg
     4501 tgtttccctg cggaagtttt tggcgtgggg ttaaatttgg tagcggggga gaggatcgaa
     4561 ctctcgacct cgcgcttatg aagcgcgcgc tctaaccagc tgagctaccc cgccgtcagg
     4621 ttgggctatt ataccttact gcggcgggaa gttcctagcc ctctcccggt cccctggtct
     4681 cagtatttga cggagcagcc gtaggcggag gtgtcggacg tggtcaccgg ttttccggac
     4741 agggcctctt ccagggcggc gcgcacgtaa ttcttagccg tggcgacatc gtccgggtcc
     4801 gtgctgggtt tatcgtcgat ggcgcccttg tagaggagca ggccctcggg gtcgatcacg
     4861 aacatgtggg gcgtggtttt ggcgccgtac atccgtccca cggtgcccgc cggatcccgc
     4921 aacagggcgg tgggagccga ctttttctcc cggaggatct tttcccacgc cgacggctca
     4981 tagtagcctt gcttgccctt ggccgacgag gcgatggcga gccacaccac gcctttcgcc
     5041 gtcatttctt tctgcagagc gggcatgttc cccgaatcgt aatgcttcct gacgaagggg
     5101 cagtccgggt tggtccactc cagaacgacg tattggccct taaaatcgct cagggacctc
     5161 ttttggccac ggacgtcttg cagggtgaag ttcggggctt tctggcctat tctgggctct
     5221 ttggcgtgtg ttggggcggc caatcccaac agcgcgaggc cggtgaacat acttttgagg
     5281 aattttttca tggggtcccc ccttcttctt ttctattaac gccggcgggg agcggcttgt
     5341 tccaattttt tttggaactc gaaccctcgc ggaggcgctt ccccctccgg cacgaagacc
     5401 agcacgccgg ccaccgccgg gacgggttcc gacggcgtct tcttggcgaa acgcaggatg
     5461 accgcgtctc tttcccgccg tatccggggt tcggacagtt tctcggcggt ggcggcggtg
     5521 gcgggataga aaaccggcgc cggcgtcccc ggcgggggtt ccggaaggga gagccgcacg
     5581 tgaacgtcgc gggggccttc cgtgaccgtg accttctccg gagaaagttc ggccgggagc
     5641 cggccgaggg cgagggcgaa cgatttcatc agcgacgcgt ccttgtcgga cggcgcgttt
     5701 tggaccggca gttccagccg cagatcggcg cggccgggga cacagatatc ggcgcattcc
     5761 agccaatcca cgcgcacctc cagcgggacc cggccttcgg ggagagcagc cgccgcttcc
     5821 agggggacca acagccagac ctcgtccttg tacccgtagc tgacgaggga cgaggccgtg
     5881 aatttgatcg gacagggcca ccggacgtcg cccgcggtga gtcccggggg caatgtccag
     5941 cgcaggcgcg tgggcatgcc ggaatctccg ggatacttcc aataggtgtg ccagccgcgg
     6001 tccatccgca ggcggacggc ggtccaaaac ttttctcccg gccggacgct gcggacgtcg
     6061 gaaaaaaatt ccgcccggac atgcggcccc tccgtgcccg ccggggcggc ggagaaaccg
     6121 agggcggaga agaagagaac ggcccgggag agggtctttt tgagaaacat gtgtcgtcag
     6181 ccggccgtcg gcgccggaag ttttcggcgg tcttccttct ggaggagttt ccccttctca
     6241 ttgaacgttt tgatgaaaac tttctggccc tgccggtaga cgacgtagga ctttttcttg
     6301 ccgttgtcgt ggtattccac ggatttgccg tgcaacacgc cgttcttgta ggaccaggag
     6361 gccatgagtt tgcccttcat ggagtaggcc cggctcacgc cgtggagttt gccgcggcga
     6421 tagttccatt cgtacatcac ctggccgttc ttgtagtagt ccaagctgag gccgtcgagt
     6481 ttgccgtaga ggtagctgta ctcggacatg agttcgccgg tggggtagct tttcttcacc
     6541 gcgccgtcgg ggacggggtc ggccgcccgc agcgcgaagg acagtcccag cgccagcgcg
     6601 gccgccagga gcaaggcgtt ccgaaatttt ttcatggttc ctcctcattt tccccttgaa
     6661 atttttcgaa cggggattaa actaacatta cggacacttc gagtgcccgt caagctggtc
     6721 cacttcaatg gacctcaagc cccgtctctg acggggcttt ttttatccgg cgggcggccg
     6781 gtccgcttcc cgactcatat ccgttccgag gcgcggttga tctcgcggat gacgccnnnn
     6841 nnnnnntcca cttcaatgga cctcaagccc cgtctctgac ggggcttttt ttatccggcg
     6901 ctcaggccgc cgccccgctc agacacgccc cgaggcgcgg ttgatctcgc ggatgacgcc
     6961 gttcaccggc aacccctgca actcttccag cgagacgggc agccggagcg tccaattgtg
     7021 gtcgccgagg gtaccgggaa aattgatccg gtaggtccag gggtcctcgt tccaggacgc
     7081 gcccaccgag agccaatcca gcaggagttg gacgctgagg atggcgatgg a
//
LOCUS       DAOTFD010000132         7000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7705_length_7000_cov_3.861771, whole genome shotgun sequence.
ACCESSION   DAOTFD010000132 DAOTFD010000000
VERSION     DAOTFD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7705_length_7000_cov_3.861771"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="P5079_07935"
     CDS             complement(<1..1049)
                     /locus_tag="P5079_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY29950.1"
                     /translation="MKFRFRLVQALLCAGFSAVLLRLGYLQLWCHDDLFLRARNQSQR
                     CIREAPRRGPILDRQGRVLAESVRVGSCYADPRLISRVDDVARRLSPILKMPVSAIAA
                     KIRKAPGSFVWLARHLPVEQAQALEKANLLGVGLQWEYKRFYPNGDLASHLLGYVGGE
                     GQGLSGTERIFNDSLLDTRPPRHALRDGRGTGLSLPSEETEGKPGGTYVRLTIDRTIQ
                     YLAEKELEWGMKRSRAKTGMIVVQDPHTGEILAAASRPNLELTAKTLSHVSEIQIPAV
                     HAVFEPGSTFKVVLAAAALEEGTVKPGETFYCENGEWRYRDLTIHDHSREKILTFAQV
                     IERSSNIGTAKVALR"
     gene            complement(1124..1390)
                     /locus_tag="P5079_07940"
     CDS             complement(1124..1390)
                     /locus_tag="P5079_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29951.1"
                     /translation="MIVPVGLGVVVMTFLATAWFEIQAVRWGYKAQKVRQEIDDLSKK
                     EETLDQRLQQNLSLSRLDALARTKFGLQAPGPAQIVLLTDPGDR"
     assembly_gap    1602..1701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2036..2866)
                     /gene="rsmH"
                     /locus_tag="P5079_07945"
     CDS             complement(2036..2866)
                     /gene="rsmH"
                     /locus_tag="P5079_07945"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY29952.1"
                     /translation="MEALRPRPGGGFLDATVGFGGHAADILARSSPDGRLWGIDRDPE
                     ALSVAESRLAVFGVRVRLARGNYDRAGELFPDVPGGTWDGILADLGVSSLQLLTPERG
                     FSFQREGPLDMRMDPAQPETAASYVERVNVEELEERLVEAGETRFAGKLARLLKSRLF
                     SSTLDLAAAVARTVPRRGRTHPATRVFMALRLAVNGEMESLKRFLTNAPAWLGPGGRL
                     AVITFHSLEDRVVKRFYKETPGFSSMKSVTKSPLIPTWEERKRNPRSRSAKLRVFEKR
                     "
     gene            complement(2935..3360)
                     /gene="mraZ"
                     /locus_tag="P5079_07950"
     CDS             complement(2935..3360)
                     /gene="mraZ"
                     /locus_tag="P5079_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRY29953.1"
                     /translation="MFLGGEYQHTLDGKRRVFIPARLRSSVARFVLTRGLEGCVSLYT
                     EGAWKKLLEKLQNLPVANKSQARAFKRLLISGATLEEVDGQGRLLVPESLGRYAGIRK
                     DVMIIGMDTHIEIWSSEKWNKYRRSAESYTNRIAEQIDL"
     gene            complement(3769..4185)
                     /gene="rnpB"
                     /locus_tag="P5079_07955"
     ncRNA           complement(3769..4185)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5079_07955"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4220..5104)
                     /gene="rnhC"
                     /locus_tag="P5079_07960"
     CDS             complement(4220..5104)
                     /gene="rnhC"
                     /locus_tag="P5079_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRY29954.1"
                     /translation="MREQRPIAHGRQLVCEGDAEKATVNVYFGKQGVSVVLGGKEGEL
                     KRMLESVRNEVLTSFGRPADLRTGSSSAVPLPPPPWIGTDESGKGDFFGPLVAAGFYA
                     GETEEKALRALGVKDSKLLSDKRIMEISDELLKGFDRKSFSVVELDPEPYNRLYQQFV
                     TEGKNLNHLLAWGHARAIENILEQKECPTVIADKFGDEAFLRSKLLRGTRDRRIRLIQ
                     IPRAEQNLAVAAASILARRRLLEWSRSAERTWGQVFPKGASPAVEAAARQFVAKHGKE
                     NLRKVAKIHFKTAERVAP"
     gene            complement(5231..5962)
                     /locus_tag="P5079_07965"
     CDS             complement(5231..5962)
                     /locus_tag="P5079_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRY29955.1"
                     /translation="MNDKIASLIKLQDFDSQLDALAQSKARLAPEKENIQSQIQSLAS
                     ALEEAKKTLTQTQVDRKNLELDVDTKEQAIRKFGNELNSVKSNDAYKALLSQIDEAKK
                     AKSQAEDQVLEMMEKIEKLQRDSKEQDKRFQQDKAAFEKQIADLDAEEQRLQGEWDQK
                     KKERDDYFQTLPDDVRTPYEYRTRGKKRDAVVAPIGVNNTCGGCRTKLPPSVVNDVMK
                     GKDLISCETCSRILYIPAKAPAATA"
     gene            complement(6005..6076)
                     /locus_tag="P5079_07970"
     tRNA            complement(6005..6076)
                     /locus_tag="P5079_07970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6042..6044),aa:Val,seq:cac)
     gene            complement(6111..6183)
                     /locus_tag="P5079_07975"
     tRNA            complement(6111..6183)
                     /locus_tag="P5079_07975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6148..6150),aa:Ile,seq:cat)
     gene            complement(6235..>7000)
                     /locus_tag="P5079_07980"
     CDS             complement(6235..>7000)
                     /locus_tag="P5079_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY29956.1"
                     /translation="LSLDTQIRNLEEAILKDKLQLIKANLRLVVSIAKKHVGTNLELS
                     DLIQEGGLGLIKAVEKFEWKRGFKFSTYATWWIRQSINRAIADQARTIRIPVHMKEII
                     SKLSKVSRKHRQELGREPTIEEYSRGLRLSPEKVRQILKIMQEPISLTTPIGEEEDSS
                     LEDFLEDKGGPTPTKSANEILRQKEVERALVTLSEREAEIIRLRFGIGTGYPRTLEEL
                     GRIFNVTRERVRQIEAKAIRKLRHPSRSRLLREYLD"
BASE COUNT         1270 a         2253 c         1935 g         1442 t
ORIGIN      
        1 aaccgcaggg ccaccttggc cgtgccgatg ttggacgacc gctcgatgac ctgggcgaag
       61 gtcaggatct tttcccggga atggtcgtgg atggtcagat cgcgatatct ccattcgccg
      121 ttctcgcagt aaaacgtctc gccgggtttc accgtgccct cttcgagcgc cgcggccgcc
      181 aacaccacct tgaacgtgga ccccggctcg aaaaccgcat ggaccgccgg gatctgtatc
      241 tcactcacat ggctcaacgt cttcgccgtc aactccaggt tcggccgact cgccgccgcc
      301 agaatctcac cggtatgggg atcctgcacc acgatcattc ccgtcttggc acgggaacgt
      361 ttcatccccc actccagttc cttctcggcc agatactgga tcgtccggtc gatggtcagg
      421 cggacatacg tgccgcccgg cttcccttcc gtctcttccg aggggagaga aagacccgtc
      481 ccgcggccgt cccgcagagc atggcggggc gggcgagtat ccaacaagga gtcgttgaat
      541 atccgctccg ttcccgataa gccctgcccc tcccccccca cgtacccgag cagatgggag
      601 gcgaggtccc cgttgggata aaacctctta tattcccact gcaaccccac ccccaggagg
      661 ttggcctttt ccaaggcctg agcctgttcc accggcagat gccgcgccag ccagacgaac
      721 gacccgggcg cctttcgtat cttggccgcg atggccgaca ccggcatctt caggatgggc
      781 gacaaccgcc gggccacgtc gtccacgcgc gagatcaacc gcggatcggc gtagcaggat
      841 cccacacgca ccgattcggc caacacgcgt ccctggcggt ccaaaatggg accccgccgg
      901 ggcgcttcac gtatgcaacg ctgggattgg tttctggctc gaaggaaaag gtcgtcatga
      961 caccacaact gaaggtagcc gagacgcagg agcaccgcgg aaaaaccggc acagaggagg
     1021 gcttgtacga gacgaaatcg aaacttcata gtaacaagag cggaccctta aaatagcccg
     1081 gcggaccgag cgccgccgaa ctccgcatca atggggattg ccattatcta tcgccggggt
     1141 ccgtcagcag gacaatctgg gcagggcccg gcgcctgcag accgaacttg gtccgcgcca
     1201 gagcatcgag ccgcgaaagg gagagatttt gctgcaggcg ctggtcaaga gtttcctctt
     1261 tcttgctcag gtcgtctatt tcctgccgca ccttctgcgc cttgtatccc caccgcaccg
     1321 cctgtatctc aaaccaggcg gtcgccaaaa acgtcatcac cacaacaccc agcccaaccg
     1381 gaacaatcaa cgacacttgt ttccagaagc gcacccgcat ctcccctcat tgacgattcg
     1441 accccccgca cagaggagtc gttagcggat aaaaaaccgt gtccaggacg agagagggag
     1501 aaggaagcct cctccctctc cgtcccggac gatgtcgttc gtgttgtcaa attaagggcg
     1561 ggtatcccgc cgagagagat acccacgccc ccaaaattcc annnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn ntccgctctt ttcgaatctc cgttggggcg cccccaaaag
     1741 ccgccttcgt ggcggttcgc ttcccaccac ccgggccgtc ttcgcggcct gacggccgct
     1801 tcacccaaac cggacgacga tgaccgagaa acctcccttg ggctccttcc catcgccgtc
     1861 gtctttcaaa ttaagggcgg gtatcccgcc gagagagata cccacgcccc caaaattcca
     1921 gtgtcacttg ggctgtccca acgctgggag atccgctctt ttcgaatctc cgttggggcg
     1981 cccccaaaag ccgccttcgt ggcggtccgt cgagcccacc gtcgacacac ttcacctacc
     2041 gtttttcaaa gaccctcaac tttgcactac gacttcgagg attccgcttt ctttcttccc
     2101 acgtcgggat caacggtgat ttcgtcacac ttttcatgct cgaaaatccg ggagtttcct
     2161 tgtaaaaccg cttcaccacc ctgtcttcca acgaatggaa ggtgatcacc gccagccggc
     2221 cgccgggtcc caaccacgcc ggcgcattcg tcaaaaatct cttaagactt tccatctccc
     2281 cattcaccgc caaccgcagc gccatgaaca cccgcgtggc cgggtgggtt cttccccgtc
     2341 gaggcactgt tcgggccacc gccgccgcca aatccagcgt gctcgagaac agccggctct
     2401 tcaacagacg ggccagtttt cccgcgaacc gggtttcgcc cgcctccacc aaccgttctt
     2461 ccaactcctc cacgttcacc cgctccacat aggaagcggc cgtctccggc tgggcggggt
     2521 ccatgcgcat gtccagcggc ccctcccgct gaaagctaaa accccgttcc ggggtcagca
     2581 attgcagcga ggagacgccg aggtcggcga ggatgccgtc ccatgtccct cccgggacat
     2641 ctgggaacaa ctcccccgcg cgatcgtaat tgccgcgggc cagccggacc cgaacgccga
     2701 acaccgccaa ccgcgattct gcgaccgaca aggcttccgg gtcacggtcg atcccccaca
     2761 accgcccgtc gggcgaggag cgggcgagga tatccgccgc atggccgccg aaaccgacgg
     2821 tcgcgtcgag gaatcccccc ccggggcgag gcctcaaggc ctccaccgcc tcctttaata
     2881 ggaccgggac atgctcaaca gggactgtca aagatcaaaa aaccgcgttt cgtgctacag
     2941 atcgatctgt tcggcgatac gattggtgta actctcagcg gaccgccggt atttgttcca
     3001 tttctccgaa gaccaaatct cgatgtgtgt atccatcccg atgatcatca cgtcttttcg
     3061 tattccggcg taacgcccta acgattcggg gaccagcagg cgcccctgcc catccacctc
     3121 ctccaacgtg gctccagaaa tcaaaagtcg cttgaacgcc cgcgcctggc ttttgttggc
     3181 gacgggcagg ttctgcagtt tctccaataa ttttttccat gccccctccg tatagagaga
     3241 cacacaccct tccaagcccc gcgtcagcac aaaacgcgcg acggagcttc tcaggcgcgc
     3301 cggaataaag acgcggcgct tcccatcgag agtatgttga tactcgccgc ccaagaacat
     3361 tcttcagtgt cccccacatt tctacactat tcaccacagt tcaccacttt ccaccaccgt
     3421 ggtcttaaat atacattgtc ccctttttcg tgtcaagcgt tttttaagat ttttttaaat
     3481 atttttcggg gagagaggcg cggggtttta tcgacggaga aaatgccggg gcggggagcg
     3541 gatccgtttt ttattaaaat atttttcaac accgatgcgc gtttcttgcg cgccatcggc
     3601 atcgcggccg aaacttgacg ggtgccacga ccgcggccgc cccccctccg cgaaaaccgg
     3661 ccaaggccgc cgggacgcgc ggcactcccc aaaaaaacgg atgagtgaaa cgggcggggc
     3721 aaaaaaatag ggccgtcgga tggtattccc attcgacggc ccgtaggcgc ctgacgacaa
     3781 cctgtacacc gagttctgtg cccgccctcg ttggacgggg tggatcattt ctctaggccc
     3841 ggactttcgt ccggggacct gggccccgcc ttgcggcggg actcctgcgg tctacctgag
     3901 gctttgggac cggagccgtc cccctttccg ccgaagcgga aggcgcctca atttgacctt
     3961 actcccggtg gggtttgccc gctccctccc ttgcgggaaa gaggcgtgag ctcttacctc
     4021 acgttttcac ccttactcca cccgaaggcg gagcggtttg ttttctgtgg cactgtccgt
     4081 acctgcgcgc cggcaggccc cgcacgttat gcggcaccgt tctcccgtgg agctcggact
     4141 ttcctctccg ttcctattca acggagcgat cctccagttg ccgttacaaa gagccgacga
     4201 ccgaaaaacg ggtgttccgt tacggcgcca cccgttcggc ggtcttaaag tgtattttag
     4261 caacttttcg caggttttcc ttcccgtgtt ttgccacgaa ctgtctcgcc gcggcttcca
     4321 ccgccggcga agcgcccttg gggaacacct gaccccacgt ccgctcggcg gaccgggacc
     4381 attccaacag ccgccgacgc gccaggatgg aggcggccgc caccgccaaa ttctgttccg
     4441 cccgggggat ctgtatcaac cgtatccgcc ggtcgcgggt gccgcgaagg agcttggacc
     4501 ggaggaacgc ctcgtccccg aatttgtcgg cgatcaccgt ggggcattcc ttttgttcca
     4561 atatattctc gatggcccgg gcatgccccc aggccagcag gtggtttaaa ttcttccctt
     4621 ccgtgacgaa ctgctgatag agccgattat acggctccgg atcgagttcc accacggaga
     4681 aggactttcg atcgaaccct ttcaacagct cgtcgctgat ctccatgatc cgcttatccg
     4741 acaacagctt gctgtctttc acgccaaggg cccgcagggc cttctcttcc gtctcgcctg
     4801 cgtaaaaacc agcggccacc aaggggccga aaaagtcccc cttgccggat tcatccgtgc
     4861 cgatccacgg cggcggaggg aggggcacgg cggaggagga ccccgtccgg aggtccgccg
     4921 gccggccgaa ggacgtcaac acctcgtttc gcacggactc caacatccgc ttcagttccc
     4981 cctctttgcc gccgaggacg acgctgacgc cctgtttccc gaagtaaacg ttcaccgtgg
     5041 ccttttcagc atcgccttca caaacgagct gtcggccgtg agcaatgggt ctttgttccc
     5101 gcacaacgag gccacggctt tcacaggccc ggacgaaacg ggacgctgcg gttgcaagcc
     5161 gatctttggg ggaggcggga tccggggaag cgggtgtggg ttgcacgaag aggccggcag
     5221 gccggggacg ttaggcggtg gcggcgggcg cctttgccgg aatatagaga atgcgggaac
     5281 aggtctcgca ggagatcaaa tccttgcctt tcatcacatc gttgacaacg ctgggcggga
     5341 gcttggtgcg gcagccgccg caggtgttgt tgacgccgat gggggcgacc accgcgtcgc
     5401 gctttttgcc gcgcgtgcgg tattcgtagg gcgtgcggac gtcgtcgggc agggtctgaa
     5461 aatagtcgtc gcgctccttc ttcttctggt cccactcccc ctgcaggcgt tgttcctcgg
     5521 cgtccaggtc ggcaatctgt ttctcgaacg cggccttgtc ctgttggaac cgtttgtcct
     5581 gttccttgga atccctctgg agtttctcta tcttttccat catctccagc acctggtcct
     5641 ccgcctggga cttggctttt ttggcctcgt cgatctgaga gaggagcgcc ttatacgcgt
     5701 cgttgctttt cacggagttc agctcgttgc cgaacttgcg gatggcctgc tccttggtgt
     5761 ccacgtcgag ttcgaggttc ttgcggtcca cctgggtctg ggtgagggtc ttcttggcct
     5821 cctcgagggc ggaggcgagg gactgtatct gcgattggat gttttctttt tccggagcga
     5881 ggcgggcttt ggactgcgcc agggcgtcca actgggaatc gaagtcttgg agttttatca
     5941 acgatgcgat tttgtcgttc acttcatcaa cctttccaca gccgcgacgc tcccatcccc
     6001 cgtgtgggcg gtacagggtt tgaacctgtg gcccttcgcg tgtgaggcga atgctctacc
     6061 gctgagctaa ccgcccgcac gggagacgga acttgcgtca aattcaaagt tgggccctgg
     6121 tggatttgaa ccaccgacct gcccgttatg agcgggatgc tctaaccgct gagctaaggg
     6181 cccgaaaatc cgcgggccgc cggccgaacc cggcggggca ggctactatt gtactcagtc
     6241 cagatattcc cgcaacaggc ggctgcggga cgggtgccgc aatttccgga tggccttcgc
     6301 ctcgatctgg cggacgcgtt cccgcgtgac gttgaaaatc ctccccagtt cctccagggt
     6361 gcgcgggtat ccggtgccga tgccgaaccg caggcggata atctccgcct cgcgttcgct
     6421 gagcgtcacc agggcccgct ccacttcctt ctgccgcaat atctcgttgg ccgacttggt
     6481 cggcgtcggg ccgcccttgt cctccaggaa atcctccagg gaggaatcct cctcctcgcc
     6541 gatgggcgtg gtgagggaga tgggctcctg catgatcttc aaaatctgcc gcactttttc
     6601 cggggaaagc cgcaggccgc gcgagtattc ctcgatggtg ggttcccgac ccagttcctg
     6661 ccggtgtttg cgcgacacct tggacagctt ggagatgatc tccttcatgt gcaccgggat
     6721 gcggatggtg cgcgcctggt ccgcgatggc ccggttgatg gactgccgga tccaccaggt
     6781 ggcgtaggtg gagaacttga acccccgttt ccattcgaac ttctcgaccg ccttgatgag
     6841 ccccaggccg ccttcctgga tcaggtcgga gagttccagg ttggtgccca catgtttctt
     6901 ggcgatggac accaccagtc tcaggttggc cttgatgagc tggagcttgt ccttgaggat
     6961 ggcctcctcg aggttgcgga tctgggtgtc gagggacagg
//
LOCUS       DAOTFD010000133         6925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7796_length_6925_cov_4.450073, whole genome shotgun sequence.
ACCESSION   DAOTFD010000133 DAOTFD010000000
VERSION     DAOTFD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_6925_cov_4.450073"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /gene="mfd"
                     /locus_tag="P5079_07985"
     CDS             <1..2517
                     /gene="mfd"
                     /locus_tag="P5079_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRY29957.1"
                     /translation="KLGGPQPAENAPATLLTYLREGGALFESVLPPEAVFDWTGPRYR
                     HADLGPADKQEPFQSPPPLGAPGTPARMNLLADTVRDWMKDGWRLYVFCHNQGEQERL
                     EEILLEQDRRFAAFLDEERISFPLGPLTRGFLDPANKRAVLANGELFGRTRRRLRLPK
                     FGGGKTLSGIVELSPGDYVVHERFGIGRYKNMERVEAGGVEADYLRLEYKGGDRVFVP
                     LFEFRQVQKFIGTDGKKPRLSSLDTSSWERTKQEVQASVAQMAAELLERAARRAAAAG
                     ISFPPDSHMEKEFGASFLYELTPDQAKAIEEMKSDMVRPRPMDRLVCGDVGYGKTEVA
                     LRAAFKAVVNNRQVAVLVPTTILAEQHGRNFRERFADYPVAIAVLSRFATAAEQKRVI
                     ADVRRGVIDIVIGTHRLLSPDIVFKNLGLVVIDEEHRFGVKQKERLMAFRDVVDVLAL
                     SATPIPRTLAASMGGVKSLSVIETPPEGRLPIATHVGAFDEKIVAAAVEHELRRGGQV
                     FYVHNRVETIEERRRWLEKSLADHGLTPKIALAHGQMSGAQLEKTMWDFLHKKYGLLI
                     STSIIESGLDIPSVNTLIVEEAEDFGLAQLYQLRGRVGRERQKAYCYLFYSPGSALTE
                     EARKRLTALKEFASLGSGFRLALRDLEIRGAGNLLGAQQHGFVNAVGIDLYGQLLTEE
                     IRRQKGGTPDKPALPEPAIELPVSAYLPEDYLPSETERIAFYRRILDAAASQLHALRE
                     ELEDRCGRLPEPAERLFEVAALRLTAREKRVTKISQTKKGLEFRFHNSVTFAPALLDD
                     LPRKHGETFSFLPGPPFGMRVAAPAENVTEWARNFLSTLG"
     gene            2536..3429
                     /locus_tag="P5079_07990"
     CDS             2536..3429
                     /locus_tag="P5079_07990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY29958.1"
                     /translation="MFIVRKSPRFVAFLAAAALLSSCGKTGPVLARVNSDPITETDFQ
                     QELASRGLAQEQYLGTLPGRKEMLELLVRRRVVLAEAKRAAFLQRPDVKKKLADMDKE
                     FEKQRREARDRVLIGDFFRYLQERDLKITDTEVRDFYERENEVKASHILLSDPETAKK
                     MRQRLLNGESFEELAKKYSEDPATGRQGGSLGYIIRGTLVPEFENALFALRVGEVSDV
                     VASPYGHHLIKKTQERKLSALPFEQTAERIRAVVEKQKFQAWIDKAKSHYKIFTDLDR
                     LSKMKLSEEPAAGGPSAGPAN"
     gene            3475..4707
                     /locus_tag="P5079_07995"
     CDS             3475..4707
                     /locus_tag="P5079_07995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY29959.1"
                     /translation="MNLKISLALVTASSLVLAGTPLPAATVVNRTIATVNGEAVLLSE
                     FDKNFNSFLEQQKNMLPAEQLTPEWEKETKKKILEQMIDDKILLQEAKKRKVRVSQRE
                     LEGGVIQIKARFLPDAGRRELDALVQKALANRPPDDQTPADINSIDLAGLWQELSKSK
                     PDAVKESESKFKEELVKEGITEKKFQDRIRDQLSVVQLTQQEVRGRTKPPADEAAKEL
                     FEKINQVMQGKEVKNLDPEDEADLESMSKFFAAQTGERVKARHILLRVDKDASFKDKS
                     AARRKLADLRAKIVAGADFSEMAQKFSDDKGSAVRGGDLGAFMRGQMVPPFEKAAFSL
                     PVGQVSDIVETDFGFHLILVDEKRAAAKLRYEDVEDDLKEYLFRVNAQKTFEEYVQQL
                     RKTASIKVLIDFDEAPKK"
     gene            4688..5740
                     /gene="pdxA"
                     /locus_tag="P5079_08000"
     CDS             4688..5740
                     /gene="pdxA"
                     /locus_tag="P5079_08000"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRY29960.1"
                     /translation="MKPPKNRGPRRTTVQRPFLALTTGDPAGIGPTVSALGAASPDLR
                     HRCRPLLVGNAWVLTHFCRLPSLKINPIADLSDYRDAPGVVNVLHVPHPAISSLVPGR
                     PQKIAGESAVLSLRTAAALALRGQVCGVVTGPVSKESFKAAGVPFPGHTELLAALCGA
                     PRVEMLLAAGPLKTLLLTRHIPLKDVPGRITTETIVSAVSFTDGRWRKMSAGRRLRWG
                     VCGLNPHAGDNGLLGAEEKRAVVPAVKRLRRRGLPVEGPFSADAVWAKHAAGAFDIVL
                     SLYHDQGMIPLKTLYPNAVVNITVGLPWVRTSPGHGTAFDLARRSPPFKNADPSATLA
                     AARWALALSQKHPPLF"
     gene            5974..>6925
                     /locus_tag="P5079_08005"
     CDS             5974..>6925
                     /locus_tag="P5079_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY29961.1"
                     /translation="MEPRRPFLSKKSRALALAAAFAATLSLPLAAYDERSRFSLGVEG
                     GLALPAAPSDFSQNYKAGADLGGRIKQDLNERWSIAASISNQKHSQKEMVSQSLVTQP
                     VLLLGLYSFTRTPSWTPYAALGLGVSRNTREVFARTLDWTKFSAAAGLGLEYNVSPLN
                     TFGIEVLYRYFTRSDPTDKNFQTITVAAVVNFYLPDSWIPEKPRKSLVPTAPLEIAAP
                     IVQEESSLQIQAQEELNQVQSDIAGKKIPPILFETGKAVILATSYETLDVVGTILRRY
                     PQFSISVEGHTDSVGSDEANLTLSQARAEAVRAYIIQNFSL"
BASE COUNT         1349 a         2467 c         2038 g         1071 t
ORIGIN      
        1 aaactgggcg ggccacaacc cgccgagaac gccccggcca ccctccttac ctatttaagg
       61 gaaggcggcg cgctgttcga atcggtcctg ccgcccgaag ccgtcttcga ttggaccggc
      121 ccgcgttacc gacacgccga cctcggcccg gcggacaagc aggaaccttt ccagtccccg
      181 ccgccgctgg gcgcgcccgg cacgccggcg cgaatgaacc ttctggccga caccgtgcgc
      241 gactggatga aggacggctg gcggctgtat gtcttctgcc acaaccaggg cgaacaggaa
      301 cgcctggaag agattttatt ggaacaggac cgccgcttcg cggcgtttct ggacgaggag
      361 aggatctcct tcccgctggg cccgctaacg cgggggttcc tcgaccccgc caacaaacgg
      421 gcggtgctgg ccaacggcga gctcttcggc cggacgcgtc ggcgtctgcg cctgcccaaa
      481 ttcggcggcg gaaaaaccct gtccggcatc gtggagctct ctcccggcga ctacgtggtg
      541 cacgaacggt tcggcatcgg gcggtacaag aacatggaac gcgtggaggc gggcggggtg
      601 gaggccgact atttgcggct ggaatacaaa ggcggcgacc gggtcttcgt gccgctgttc
      661 gagttccgcc aggtgcagaa gttcatcggc accgacggaa aaaaaccgcg cctctcctcc
      721 ctcgacacct cctcctggga acgcaccaaa caggaggtgc aggcctcggt ggcgcagatg
      781 gcggcggaac tgctggaacg cgccgcgcgc cgggccgcgg ccgcgggcat ctcttttccg
      841 cccgactcgc acatggaaaa ggagttcggc gcctcgttcc tttacgagct caccccggac
      901 caggccaagg ccatcgaaga gatgaagagc gacatggtgc ggccgcggcc catggaccgg
      961 ctggtctgcg gcgacgtggg ctacggcaag accgaagtgg ccctgcgcgc cgccttcaaa
     1021 gcggtggtga acaaccgcca ggtggcggtg ttggtgccca ccaccatcct ggccgaacag
     1081 cacggccgca acttccgcga acggttcgcc gactaccccg tggccatcgc ggtgctctcg
     1141 cggttcgcca ccgcggccga acagaaacgc gtcatcgccg acgtgcgccg cggcgtcatc
     1201 gacatcgtca tcggcaccca ccggctcctt tcgcccgaca tcgtcttcaa gaacctcggc
     1261 ctggtcgtca tcgacgagga acaccgcttc ggcgtgaagc agaaagaacg cctcatggcc
     1321 ttccgcgacg tggtggacgt gctggcgctg tcggccacgc ccatcccgcg gaccctcgcc
     1381 gcctccatgg gcggggtgaa gtccctctcc gtcatcgaga ccccgccgga agggaggctc
     1441 cccatcgcca cccacgtggg cgcgttcgac gagaagatcg tggccgccgc ggtggaacac
     1501 gaactgcggc gcggcgggca ggtgttctac gtgcacaacc gcgtggagac catcgaggaa
     1561 cgccgccgct ggctggaaaa gtccctcgcc gaccacggcc ttacccccaa gatcgccctg
     1621 gcccacggcc agatgagcgg cgcccaactg gagaagacca tgtgggactt cctccacaag
     1681 aaatacggcc tgctcatctc caccagcatc atcgagtccg ggctggacat cccctccgtc
     1741 aacaccctca tcgtcgaaga ggcggaggat ttcggcctgg cccaactcta tcagctccgg
     1801 ggccgcgtgg ggcgcgaacg ccaaaaggcg tactgctacc ttttctactc gccgggttcc
     1861 gccctcaccg aggaggcccg caaacgcctc accgcgctca aggagttcgc ttcgctgggg
     1921 tccgggttcc ggctggccct gcgcgacctg gagatccgcg gcgcgggcaa cctcctcggc
     1981 gcccagcagc acgggttcgt caacgccgtg ggcatcgacc tctacggcca gctgctcacc
     2041 gaggagatcc gccggcagaa gggcggaacc ccggacaaac ccgccctgcc cgaacccgcc
     2101 atcgaactgc cggtgtccgc gtacctgccc gaggactatc tgccgtcgga gacggaacgc
     2161 atcgcctttt accgccgcat cctggacgcc gccgcctccc agctccacgc cctgcgggaa
     2221 gaactggaag accgctgcgg ccggctgccg gaacccgccg aacggctctt cgaggtggcc
     2281 gcgctgcggc tcaccgcccg ggagaagcgc gtcaccaaga tcagccaaac gaagaaaggc
     2341 ctggagttcc ggttccacaa cagcgtcacc ttcgcccccg ccctgctgga cgacctcccc
     2401 cgaaaacacg gggagacctt ctccttcctg cccggcccgc cgttcggcat gcgcgtggcg
     2461 gctccggcgg agaacgtgac ggaatgggcc agaaacttct tgtcaacgtt gggctaattc
     2521 gatagactga gcgctatgtt tatcgtccgt aaaagtccgc gtttcgtcgc gttcctggcc
     2581 gccgccgccc tgttgtcgtc ctgcgggaag acgggccccg tgctcgcccg cgtcaactcc
     2641 gaccccatca ccgaaaccga ttttcagcag gaattggcct cccgcggact ggcgcaggaa
     2701 cagtatctgg ggacgctgcc gggccgcaag gagatgttgg aactgctggt gcgccggcgg
     2761 gtggtgctgg cggaagccaa acgcgccgct ttcctgcaac ggccggacgt gaagaaaaaa
     2821 ctggcggaca tggacaagga atttgaaaaa cagcgccggg aggcccgcga ccgcgtgctg
     2881 atcggcgatt tcttccggta cctgcaggaa cgcgacctca agatcaccga cacggaggtg
     2941 cgcgatttct acgagcggga gaacgaggtg aaggccagcc acatcctgct gagcgacccc
     3001 gaaacggcga agaaaatgcg ccagcgcctc ctcaacggcg aatccttcga ggaactcgcc
     3061 aagaaatatt cggaagaccc cgccaccggc cggcagggcg gcagcctggg ctacatcatc
     3121 cgcggcacgc tggtgcccga attcgagaac gccctcttcg ccctgcgcgt gggcgaggtg
     3181 tcggacgtgg tcgcctcccc ctacggccac cacctcatca aaaaaacgca ggaacgcaaa
     3241 ctttccgccc ttccgttcga gcagaccgcc gaacgcatcc gcgccgtggt cgaaaaacag
     3301 aagttccagg cctggatcga caaagcgaag tcccactaca agatcttcac cgacctcgac
     3361 cggctgagca agatgaaact gtcggaagag ccggcggccg gcggaccgtc ggccggcccg
     3421 gccaactgac cgcctctccc gattttttcc gataactcta aggaggtaac acccttgaat
     3481 ctcaaaattt cccttgcgct ggtcaccgcc tcgtcgctcg ttctggcggg gacgcccctg
     3541 cccgccgcca ccgtcgtcaa ccgcaccatc gccaccgtca acggcgaggc ggtgctgctc
     3601 tcggaattcg acaagaactt caattccttc ctcgaacagc agaagaacat gctgccggcg
     3661 gaacagctca ccccggaatg ggaaaaagag acgaaaaaga agatcctgga acagatgatc
     3721 gacgacaaga tccttctgca ggaggccaaa aagcgcaagg tgcgcgtgag ccagcgcgag
     3781 ctggaaggcg gcgtcatcca gatcaaggcg cgtttcctgc ccgacgcggg ccggcgcgaa
     3841 ctggacgccc tggtacaaaa agccctcgcc aaccgcccgc cggacgacca gacccccgcc
     3901 gacatcaaca gcatcgacct ggcgggactt tggcaggaac tctccaagag caagcccgac
     3961 gcggtgaaag aatcggaaag caagttcaag gaggagctgg tcaaggaagg catcaccgag
     4021 aaaaaattcc aggaccgcat ccgcgaccag ttgagcgtgg tgcagctcac gcagcaggaa
     4081 gtccgcggcc gcaccaagcc ccccgccgac gaggccgcca aggaactctt tgagaagatc
     4141 aaccaggtga tgcagggcaa ggaagtgaag aacctcgacc cggaagacga agcggacctg
     4201 gaatccatgt cgaaattctt cgcggcccaa accggcgaac gcgtgaaggc ccggcacatc
     4261 ctcctccgcg tggacaagga cgcctccttc aaggacaaat ccgccgctcg gcgcaaactg
     4321 gcggacctcc gcgccaaaat cgtcgccggc gccgacttct ccgaaatggc ccagaaattc
     4381 tccgacgaca agggcagcgc cgtccgcggc ggggacctgg gcgccttcat gcgcggccag
     4441 atggtccccc ccttcgaaaa agccgccttc agcctgccgg tcggccaggt gtccgacatc
     4501 gttgagacgg acttcgggtt ccacctcatc ctggtggacg aaaaacgcgc cgccgccaaa
     4561 ctccgctacg aagacgtgga ggacgacctc aaggaatacc tcttccgcgt caacgcgcaa
     4621 aaaacgttcg aagaatacgt ccagcaactc aggaagaccg cttccataaa ggtcctcatc
     4681 gactttgatg aagcccccaa aaaatagggg accccgccgc acaaccgtcc aacgcccttt
     4741 cctcgccctc accacgggcg atcccgccgg gatcggcccc acggtgtcgg ccctcggcgc
     4801 cgcctcgccg gacctgcgcc accgctgccg gccgctgctc gtcggcaacg cctgggtcct
     4861 gacgcatttt tgccggcttc cctcccttaa aatcaatccc atcgccgacc tgtcggacta
     4921 tcgcgacgcc cccggcgtcg tcaacgtgct gcatgtgccg cacccggcca tctcctcgct
     4981 cgttcccggc cggccgcaaa agatcgccgg agaaagcgcg gtactgtccc tccgcaccgc
     5041 cgcggccctg gccctgcgcg gacaggtgtg cggcgtggtc accggaccgg tctccaaaga
     5101 atccttcaaa gccgccgggg tcccgtttcc cggccacacc gaactgctgg ccgccctctg
     5161 cggcgcgccc cgggtggaga tgctcctggc cgccggcccg ctcaaaaccc ttctgctgac
     5221 gaggcacatc cccctcaaag atgttccggg ccggatcacc acggaaacga tcgtctccgc
     5281 cgtgtcgttc acggacggcc ggtggcggaa aatgtcggcg ggccggcggc tccgctgggg
     5341 cgtgtgcggg ctcaacccgc acgccggcga caacggcctt ctgggcgcgg aagagaagcg
     5401 cgccgtcgtt ccggcggtga aacgactccg ccgccgggga ctcccggtgg aaggcccctt
     5461 ctcggccgac gcggtgtggg ccaaacacgc ggccggggcc ttcgacatcg tcctctcgct
     5521 ctaccacgac caggggatga tccccctcaa aacgctctat cccaacgccg tggtcaacat
     5581 caccgtgggc ctgccgtggg tgcgcacctc gccggggcac ggcacggcgt tcgacctggc
     5641 ccgccgctcg cctcccttca agaacgcgga cccctcggcc accctggccg cggcccgctg
     5701 ggcgctcgcc ctctcacaga agcacccgcc tctcttctga actcccaaag gcctcccgcc
     5761 ctcgaccctg gtgcagcgtt tcttgggcac cctgcacaaa ggaggacacc ccggcgaagt
     5821 aaacaatccg attgcttcac ttcgttcgca atgacgacaa aaggcccgcc ggacaatttt
     5881 tcgaaacccc ttcagggcgt ttcgagggag atgacatccc ctcaaacagg ggttccttgc
     5941 caaacaccgt ctgaatttgg cacaatcgaa acgatggaac ctcgacggcc tttcctctcg
     6001 aaaaaatcgc gcgccctcgc cctggcggcc gcgttcgccg cgacgctctc ccttccgctc
     6061 gccgcctacg acgagcgttc ccgcttttcc ctcggggtcg aggggggcct cgccctgccg
     6121 gcggcgcctt ccgatttttc ccagaactac aaagccgggg ccgacctcgg cgggcgcatc
     6181 aagcaggacc tgaacgaacg ctggtccatc gccgcctcca tctcgaacca aaaacattcc
     6241 caaaaagaga tggtctccca gagtctcgtc acccagcccg tcctcctgct gggcctgtat
     6301 tcgttcacgc gcacccccag ttggacgccg tacgccgccc tggggctcgg cgtgagccgc
     6361 aacacgcgcg aggtgttcgc ccgcacgctg gactggacca agttctccgc cgccgccggg
     6421 ctggggctgg aatacaacgt ctccccgctc aacacgttcg gcatcgaggt gctgtaccgt
     6481 tatttcaccc gttcggaccc caccgacaag aatttccaga ccatcacggt cgcggcggtg
     6541 gtgaatttct atctgccgga ttcctggatc cccgaaaaac cccgcaaatc cctcgtgccc
     6601 accgcgcccc tggagatcgc cgcccccatc gtccaggagg aatcctccct gcagatccag
     6661 gcgcaggagg agctgaacca ggtccagtcg gacatcgcgg gcaagaagat cccgcccatc
     6721 ctctttgaga ccggcaaagc ggtcatcctg gccacctcct atgagaccct cgacgtcgtc
     6781 ggcaccatcc tgcgccgata tccccagttc tccatcagcg tggaaggcca caccgacagc
     6841 gtcgggtcgg acgaagccaa cctcaccctc tcccaggcgc gggccgaagc cgtgcgcgcc
     6901 tacatcatcc agaacttctc cctgc
//
LOCUS       DAOTFD010000134         6890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7846_length_6890_cov_3.267740, whole genome shotgun sequence.
ACCESSION   DAOTFD010000134 DAOTFD010000000
VERSION     DAOTFD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7846_length_6890_cov_3.267740"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="P5079_08010"
     CDS             <1..963
                     /locus_tag="P5079_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY29962.1"
                     /translation="GIKGYIATTFKETGQEIDVRVQLRPEDRAQTDDIRRLTIRAPSG
                     VMVPLAEVASLELARGPSEIKHLDQQRAILLTANVLKRSVADVIQDVQGLLDTYQTLS
                     DYNITLTGESAQIKESFGGLSIAMVMSVLLIYMIMAAEFESFGQPLIIMTTVPLCVVG
                     VALTLFVTRIPVSAPVILGTIILGGLVVNNGIILIDFMNALREEAGVTDLREVVMRGG
                     ATRLRPIVMTMLVSILGVLPMALGLSEGSELSSPMASVTFGGLLVSACLSLFVIPWLY
                     FNFERWQLRNAPQEESVTVESAPTDESGGDAASHSTGTDTINSP"
     gene            990..4385
                     /locus_tag="P5079_08015"
     CDS             990..4385
                     /locus_tag="P5079_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY29963.1"
                     /translation="MSSTDTDPSPSSGTSLCSLAITRPIATLMVFIAASLLGSFSIFH
                     LPVDLMPNSGAGSLTVFIGVRGGLPPEDIESLVTKIVEEAVSATSKLQSLMSVSRKDR
                     SVTTLIFEPGTNVAFSALEVQERLAKVRGKLPKDIEKPIVAHYEENDYPIMIVALTSD
                     KYTPEMLRDMVDNTLKPQLMRIDGVGNIEIGGGRQRKILVEFDQQRLEAYQLPIRQII
                     GQLGENNLNLLTGKTKDERNSYYVRTMGQFQTMDDIKKMTVAVTKEGSRVRVGDIAEV
                     RDFYLEPDSYARLNKKPTVSVYIQKESQTNTIRTAKKINETLKKFQENLDPEITLTIV
                     SNQAIFIQSAISNVTTALWQGLLLTALILWVFIRDIKHTAIILVSIPLSVLLTFGLMY
                     LCKIGINVMTLSGIALGIGMLVDNSIVVLESVVEAKEHFFKHHPDVSEVPRTVSDPLI
                     SEASQKMTLPILASTLCTVIVFIPILFINKQVQILYTGLALTVTFSLVVSFFVALTAV
                     PLLSALIPMRREEPGAKHFDIHELWYEYISEKPFPERLDRWMDSLKNVFTRTVHFLKH
                     PPVEAGPAEEPPEARPEGGGEPEPSRRARLKRHLLLFVAHYVSPLFKSPMRLYRNLMS
                     AAVRYRYQMLFWIFVAFLVSGFVYTQFLEKDFMGSTEPNEFIIFVELPAGAKLDISDQ
                     VVAEVEKILSDTPEIQKVVKTAAARVEGWSSKIYVTLVPQAERTRPVQDIINDLRPRV
                     AEIGAVYDTFIYFSEPESSKELLVDVYGFDYNVLRDVAVGIAKRLQNVKGLTDIKLRY
                     KPGRPEVKIMVDKDRAALFGFTVKDIAETLHAQIRGLRATYFYTATEQIETVARLQEK
                     HRKTLEDLNSLTLMTNDGTIVPLQQVSTLEFSLTPSEIWRKDKQRMIQVSSNREKLAL
                     STAVKKSAVHLKGMDVPQNYYYQFGGDYQQMIKNEQEFKFAFLIMVGLVYIVLACLFE
                     SYAQPLLIMLTVPLAIISVVPVLYFSGTSVTMGTYLGLIMLGGLVVTNAIILLDRLNA
                     IQPGENLLRAVMLVGKQRLKAITLVQLTTILDLLPMIWDKSESAGLWSPLAISVVGGI
                     TGATILTLFVIPGCFLMIKDYENYKNRAEYPYSEEEIPVPVSE"
     gene            complement(4466..4840)
                     /locus_tag="P5079_08020"
     CDS             complement(4466..4840)
                     /locus_tag="P5079_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29964.1"
                     /translation="MLKRILRILVPALVIGSAAAAAYYFYHRPAEEQSDETQAAVSQE
                     DRSPEAVAKDQATEQRRRLEETLRGAEAARRLAGDARPDLSPGVVNPVENLQRTFQTI
                     DEINRINRFNREMKEKRTPSRP"
     gene            complement(4886..5131)
                     /locus_tag="P5079_08025"
     CDS             complement(4886..5131)
                     /locus_tag="P5079_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29965.1"
                     /translation="MRDKNWTLMKINFIFKIFGWIAAALGVVFFFIILIGGGTPEAPR
                     ATSILALVLGFFYFVFFYTVAEVLRLLMDIEANTRKE"
     gene            5600..6280
                     /locus_tag="P5079_08030"
     CDS             5600..6280
                     /locus_tag="P5079_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29966.1"
                     /translation="MLLFFSTSGLNADRPYSTTRPPKTAFSSPDSQKETTASTDTSKG
                     TQWRKKSRVRPGGPLFTSPETGAATENKSKFVPGKAANAPRADTVQGTSAQKENPEAQ
                     WRINPGDRRWETWRELPPSQQIDLWRDLTLEEKNRYWDAMNVHERATLWTKMDPEEKE
                     RCWDRLPETDRRALCDNMPPKERALWSPRLAANTGSVGEDKGAAKFKPAKPLPGYQPK
                     SNYTEEDE"
     gene            6439..>6890
                     /locus_tag="P5079_08035"
     CDS             6439..>6890
                     /locus_tag="P5079_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY29967.1"
                     /translation="MSEKKKILIVDDDSRITDLLSDFCGDHGYEVKTLNDSRQVVSTA
                     MAWRPDLITLDLEMPERTGLEVLQEIRGKLQENQTLLDSETNLVIKAAAEKRQALLHQ
                     DLANMERRIAIESREHALRSRQKTEPLAVLKTFLVSLKGDEAAIQNRA"
BASE COUNT         1520 a         2232 c         1863 g         1275 t
ORIGIN      
        1 ggcatcaagg gatacatcgc cacgactttc aaggaaaccg gccaggagat cgacgtccgc
       61 gtgcagctcc gtccggagga ccgggctcag accgacgaca tccgtcggct caccatccgg
      121 gcccccagcg gcgtcatggt gcccctggcc gaagtcgcga gcctggaact ggcccgaggc
      181 cccagcgaga tcaagcacct cgaccaacaa cgcgcgatcc tgctcaccgc gaacgtgctc
      241 aaacgcagcg tggcggacgt cattcaagac gtgcagggac tcctcgacac ctatcagacc
      301 ctcagcgatt acaacatcac cctcaccggc gagagcgccc agatcaagga gtccttcggc
      361 gggctctcca tcgccatggt catgtccgtc ctgctcatct acatgatcat ggccgccgag
      421 ttcgagtcct tcggccagcc gctcatcatc atgaccaccg tgccgctctg cgtcgtcggg
      481 gtggcgctta ccctgttcgt cacgcgcatc ccggtgagcg cccccgtcat cctcggcacc
      541 atcatcctcg gcggactggt ggtcaacaac ggcatcatcc tcatcgactt catgaacgcc
      601 cttcgggagg aagcgggggt cacggacttg cgggaggtcg tcatgcgggg aggcgccacc
      661 cgcctgcggc ccatcgtcat gaccatgctg gtgtccatcc tcggcgtcct tcccatggcg
      721 ctggggctgt ccgaaggatc ggaactctcg tctcccatgg cctcggtgac cttcggcgga
      781 ctgctggtgt ccgcctgcct ctctctcttc gtcatcccct ggctttactt caactttgag
      841 cggtggcagc ttcggaacgc tccccaagag gaatcggtca ccgtcgaatc cgccccgacg
      901 gacgaatccg gaggagacgc cgcttcccat tccacgggga cagacaccat caactctcct
      961 tgactttttc ttaaaccgac cgcacgccca tgtcctccac cgacaccgac ccctcccctt
     1021 cttccggcac gagtctttgc agtctcgcca tcacccgccc catcgcgacc ctgatggtct
     1081 tcattgccgc ctcgttgctg gggtcctttt ccatcttcca cctgcccgtg gacctgatgc
     1141 ccaattccgg cgccggctcg ttgacggttt tcatcggggt gcgcggcggt ctgccgccgg
     1201 aagacatcga gtccctggtg acgaaaatcg tcgaagaggc ggtcagcgcc accagcaaac
     1261 ttcagagcct catgtcggtg tcccgaaagg accgctccgt caccacgctc atcttcgaac
     1321 cggggaccaa tgtggccttt tccgccctgg aggttcagga acgcctcgcc aaggtgcgcg
     1381 ggaaactccc caaagacatc gaaaaaccca tcgtcgccca ctacgaggaa aacgactatc
     1441 ccatcatgat cgtggcgctc accagcgaca agtacacacc ggagatgctg cgcgacatgg
     1501 tggacaatac cctcaaaccc cagctgatga ggatcgacgg tgtcggcaac atcgagatcg
     1561 gcgggggacg gcagcggaaa atcctggtgg aattcgacca gcagcggctg gaggcctatc
     1621 agctccccat ccggcagatc atcggtcaat tgggggaaaa caacctcaac ctgttgaccg
     1681 ggaaaacgaa agacgagcgc aactcctact acgtccgcac catgggccag ttccagacca
     1741 tggacgacat caagaagatg acggtggcgg tgaccaagga ggggtcccgt gtgcgcgtcg
     1801 gcgacatcgc ggaggtccgc gatttctacc tggaaccgga cagttatgcc cgtctcaaca
     1861 agaagccgac ggtctccgtc tatatccaga aggaatccca gaccaacacc atccgcaccg
     1921 caaaaaaaat aaacgagacg ctcaaaaaat tccaggagaa cctcgacccg gaaatcaccc
     1981 tcaccatcgt ctccaatcag gccatcttca tccaaagcgc catcagcaac gtcaccaccg
     2041 ccctttggca ggggctcctc ctgacggccc tcatcctgtg ggttttcatt cgggacatca
     2101 agcacacggc cattatcctc gtctccatcc cgctctcggt gcttctgacc ttcggcctga
     2161 tgtacctgtg caaaatcggc atcaacgtca tgaccctgtc cggcatcgcc ctgggcatcg
     2221 gcatgctggt ggacaactcc atcgtggtgt tggaaagcgt cgtggaggca aaggaacatt
     2281 tcttcaaaca ccaccccgac gtctcggagg tcccgaggac agtctccgat cccctgatca
     2341 gcgaggccag ccaaaaaatg acgctcccca tcctggcctc gacgctgtgc acggtgatcg
     2401 tgttcatccc catcctcttc atcaacaaac aggttcaaat tctctacacc ggcctggcgc
     2461 tgacggtcac cttctccctc gtggtttcgt tctttgtggc gctcaccgcc gtcccgttgc
     2521 tgagcgcgct gatccccatg cgccgggagg agccgggggc gaagcatttc gacatccacg
     2581 aactctggta cgaatatatt tccgaaaaac cgttccccga aaggctggac cgctggatgg
     2641 acagcctgaa aaacgttttc acgcgaacgg tccatttcct caaacatccc ccggtggagg
     2701 ccggccccgc ggaggaaccg cccgaagccc gaccggaggg gggaggagaa ccggagccct
     2761 cccggcgagc gcgcctcaaa cggcatttat tgcttttcgt ggcgcattac gtctccccgc
     2821 tgttcaaaag cccgatgagg ctttatcgga acctcatgtc ggccgcggtg cgctaccgtt
     2881 atcaaatgct cttttggatt tttgtcgcct ttctcgtctc ggggttcgtc tacacccaat
     2941 tcctggaaaa agatttcatg ggctcaacgg aaccgaacga gttcatcatc ttcgtggaac
     3001 tgccggccgg agccaaactg gacatctccg accaggtggt ggccgaggtg gaaaaaatac
     3061 tgagcgatac gccggagatc cagaaagtgg tgaagaccgc ggcggcgcgg gtggaaggct
     3121 ggtcgtccaa gatctacgtc accctggtgc cgcaggcgga acgaacccgg cccgtgcagg
     3181 acatcatcaa cgatctccgc ccccgcgtgg cggagatcgg cgccgtgtat gacactttca
     3241 tctacttctc cgaaccggag agctccaagg aacttctggt ggacgtatac gggttcgact
     3301 acaacgtgct gcgggatgtg gccgtgggca tcgccaaacg gctgcaaaac gtcaaggggt
     3361 tgacggacat caaacttcgc tacaaaccgg gccggccgga ggtgaagatc atggtggaca
     3421 aggaccgcgc cgccctcttc ggcttcaccg tcaaggacat cgccgagacg ctccacgcgc
     3481 agatccgcgg cctccgcgcc acttatttct acaccgccac cgagcagatc gaaaccgtgg
     3541 cccggctgca ggaaaagcat cgcaagacgc tggaagacct caactccctc accctgatga
     3601 cgaacgacgg caccatcgtc ccgctgcagc aggtcagcac cctcgaattc tccctcacgc
     3661 ccagcgaaat ctggcgcaaa gacaaacagc ggatgatcca ggtcagctcg aaccgggaaa
     3721 aactggcgct cagcaccgcc gtgaaaaagt cggcggtgca tctgaaaggg atggacgttc
     3781 cgcagaacta ttactaccaa ttcggcggcg actaccagca gatgatcaag aacgagcagg
     3841 aattcaagtt cgccttcctc atcatggtgg ggctggtcta catcgtcttg gcctgcctgt
     3901 tcgagtccta cgcccagccc ctgctgatca tgctgacggt gcccctggcc atcatctcgg
     3961 tggtgccggt gctctatttc tcaggcacca gcgtgaccat ggggacgtat ctcggcctca
     4021 tcatgctcgg cggactggtc gtcaccaacg ccatcatcct gctggaccgc ctgaacgcga
     4081 tccagccggg cgagaacctc ctgcgcgccg tcatgctcgt cggaaaacaa cgcctgaaag
     4141 cgatcaccct ggtgcagctg accaccatcc tggacttgtt gccgatgatt tgggacaaga
     4201 gcgaatcggc cggtctctgg tcgccgctgg ccatctccgt cgtcggcggc atcaccggcg
     4261 ccacgatatt gacgcttttc gtcatccccg gctgttttct catgatcaag gactacgaga
     4321 actacaaaaa ccgggcggaa tacccctact cggaggagga aatcccggtc cccgtttccg
     4381 aatgatttat ccgtgacaaa agaaaaaggg cggatcgttt ttaaaaacga tccgcccttt
     4441 taaatttccg gcagaagttc cgcgatcagg gccgacttgg ggtacgcttc tccttcattt
     4501 cacgattgaa ccggttgatg cggttgatct catcgatggt ttggaaggtc cgttgaagat
     4561 tctcaacggg gttaacgacc cccggggaca ggtccgggcg ggcatccccg gcaagacgcc
     4621 gggccgcctc cgccccgcgc aaggtctcct ccaaccgccg ccgctgctcc gtcgcctggt
     4681 ccttggccac cgcttccggc gaccgatctt cctgggaaac cgcggcctgc gtttcgtccg
     4741 actgttcctc ggccggccta tggtagaaat aataggcggc cgccgcggcc gatccgataa
     4801 caagcgcggg gacgagaatc cggaggattc tcttaagcac gggaggttat ctgcctttcc
     4861 cccgaacggg ggacgtgaag gggaattatt ccttgcgcgt gttggcttcg atgtccatca
     4921 acaaacgaag gacttcggcc accgtgtaga agaagacaaa ataaaagaag cccagaacca
     4981 gcgccaagat ggacgtggcc cgcggcgctt ccggcgtgcc gcctccgatg aggatgatga
     5041 agaagaacac cacgcccaac gcagcggcga tccaaccgaa aatcttgaag atgaagttaa
     5101 tcttcattaa cgtccagttc ttatctctca tgctgatgat tcctcctcac agtgggaacg
     5161 cgggcggcgc ccggttcgta ttcaagcggt taagtaacca ttgaaagcca atgatcttaa
     5221 ccggcaaaga atttataaca aaccctgcgc cctgtcaagt cttattcacc cctcgaaaat
     5281 ctctcttcac gttttccggg aaacgtcttc cccggcaaaa cgaggactct cgggcccgga
     5341 ggaggcccct cccgccctgc ggtcccaaca caagaagcgg agccggcccg accttcccca
     5401 gagacccctt cttcctgaac gcggtgggaa acaaggatga gcgacgaaga ggccccccgg
     5461 tccccggctt tccaccgttc cccacaacat gtccacaggt tacaaacaac ctattccgcc
     5521 gttatcccca aaaaagcttg ccaaacggct ttcgccgctt taaactagtg ctatgccaac
     5581 tcgcttgggg tctattctgt tgcttctgtt ctttagcaca tcgggtctga acgccgaccg
     5641 cccctattcc accacccgtc ctcccaaaac ggctttctcc tcgcccgact cgcaaaaaga
     5701 aaccaccgcc tccaccgaca cgtcaaaggg gacccaatgg aggaaaaaaa gccgggtccg
     5761 tcccggaggt cccctcttta cctccccgga aacgggcgcc gccaccgaaa acaaaagcaa
     5821 attcgttccg ggcaaagccg ccaacgcgcc ccgggccgac acggtccaag gaacctccgc
     5881 ccaaaaagaa aatccggaag cccaatggcg aatcaatccg ggggatcggc gctgggaaac
     5941 atggcgggaa ttgcctccca gccagcagat agacctttgg cgggatttga ccttggaaga
     6001 aaaaaatcgt tactgggacg ccatgaacgt gcacgagcgg gccacgctct ggaccaagat
     6061 ggacccggaa gagaaagagc ggtgttggga ccggctccct gaaacggacc gacgggcgct
     6121 ttgcgacaac atgccgccca aagaacgggc tctttggagt ccgcggctgg cggcgaacac
     6181 cggaagcgtc ggggaggaca agggggccgc taaattcaag ccggccaaac ctctcccggg
     6241 gtatcagccg aaatccaact acacggagga agacgagtag ccggccctcc ggagtcccta
     6301 aaacacgccc gggcgaaccc ctccggttac aataatttta ccgatccgtt tccagacagt
     6361 tgcaggttgt taaggccttt ttcggttctc cggggatagg cttaaaatgg cgcaatagag
     6421 cggggggcgg cgatatttat gtccgaaaaa aagaaaatcc tcatcgtcga tgatgattcg
     6481 cgcatcaccg acctattgtc cgacttctgc ggcgaccacg gctatgaggt caaaaccctg
     6541 aacgacagcc ggcaggtcgt ctcaacggcc atggcgtggc ggccggacct catcacgctc
     6601 gacctggaaa tgcccgaacg caccggcctg gaggtcctcc aggagatccg cggcaagctc
     6661 caggaaaatc agaccctgct cgacagcgag accaacctgg tcatcaaggc ggccgcggaa
     6721 aaacgccagg ccctgctgca ccaggacctg gccaacatgg aacgccggat cgcgatcgaa
     6781 tcccgcgagc acgcgctgcg cagccgccag aaaaccgagc cgctggcggt gttgaaaacg
     6841 ttcctggtct ccctgaaggg cgatgaggcg gccatccaga accgggccaa
//
LOCUS       DAOTFD010000135         6834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_7946_length_6834_cov_3.838177, whole genome shotgun sequence.
ACCESSION   DAOTFD010000135 DAOTFD010000000
VERSION     DAOTFD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7946_length_6834_cov_3.838177"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6834
                     /locus_tag="P5079_08040"
     CDS             <1..>6834
                     /locus_tag="P5079_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY29968.1"
                     /translation="HLVIDGSTLTVQPGDGLVLNGNWTEQSGGVFSPAVGSTVAFTGT
                     SVISMLPGSSFYHLLVDGAVDVKDATDVTIRGNLTLNSGVFTVTNSTVSVAGHIPTGG
                     GSLGHGANSTYVFNGAANQTIACPDFRNIVSQNNGNTVYFPDLSNVYGDFTLMGGGMA
                     DFNNRNVSFYGSFERYGTPVNLATATFVGGSNAQFNCNDPFLSDLVVAKNSGAALSAG
                     DGMNLDGDFLVQGGTFVPGAYVHSLAGNFIVTGGFLETDEGKFVFDGSGDQALSWTGG
                     NLVLCDVDVNGGASVTQTGGGLLISTLTVNAGGFYNGSNQGTRIDGGINNSGNLIGLI
                     ATFESSNTKVINSTTTPFSLTAVQVQEGVLQSNVALTVPGGFGVSAGSTFTAVNSTFV
                     HTLTGLSNSGKFSVTGGTVTFNGAGTNDVFAGDNTFSHLLLNMGAGGLLQVLSGSLNV
                     DGNFTLAGGTFTLNDATHTFAGNFTQMLGTTLTANGSTIKFDGSETKSFSAMGPFYNV
                     VVANTGGDLALEGDVDINGSLIIESPSGSIDWSDAGRTVRLAGDFIDNNNFSQAVDSK
                     LIMDGDACQHVGGSSTNMLVNFEINSSSTVEILTAKTFENVAILGGTMTLAVSNINVR
                     GDWNHTGGGFLPNGSTVTFTGGVPMNGQSRTVQSVSGSAFNHFSVNATTAIAITSDLG
                     VTGNFTLLGGTVTLDGYGLEVGGDWTESAAVRFRSAGGTVTFRGGGAQSVTQSPSSAF
                     GTLEHANNSNLVANSDLNIGGNFYPNTGSFDTNGFEVRLKGDFLTPSGTFNPSGTWVF
                     QGSGAQNVAQDVFDNVDIQTGGTLVFTNPNGLKTNDAGGTGHLTIGPAATVGFGGNDL
                     EVAGNMLSSGTISGVGLTTFSGAANQSVWLTPSSSLGYTYVNKTGGLLTATTGLTFLD
                     SLNIVNGGFDLGLGTHEVYGFGYIGSGGPSTLNVSSAAVFFHENVSVTNGSTLILPDS
                     VSGGKLALASGKTLQVASGEFVSQSVYNMVGSTQSGVYVNFVVQSGSTINVSGMTLGQ
                     LGDNGLEIQSGAKVAELNNVNYREFKPGSKGLYLNCELVSPATFTAHDFDSSITTNVY
                     LADNIFPAYIYMQGATGVSSGPAREYDPHNRVFWGVPTEPSAPAALAASATSLQWSWT
                     NNSQDFEGTKISSMSSGENYLLSAGTSTTSQYEYILSTNTLYTRYIYAYSPIGESLRV
                     STQAYTWAALPGGSASTFTAVGATILTVNWTDGGNPAGTTYIADISTGPFPNSYADNK
                     SSTTLNTYAVFSGLDPITVYYAQVRAVNHGGVETGELPLGSTQTENAVIWTGASSTSS
                     LASDVDNWLSNARPVNDVRVIFNAPGSVQANCQWDLPEVSIMELRLEGTFNGTVTLTS
                     SMTATGDVVLDAGTFDSNNRNMSVGGDWGVNGALYNSGGGGAILFNGVSSQTADLDYP
                     LPIVRSENAAGLTLGSSAGTMQVGGLGVEPGAQLALASGKTLLISGNFSSSGTFNNNG
                     STVSFGGTGSVWAVAGTTFACVSVNAGTLSAANALDVNGHLRISGGVFNAGAFNHALA
                     GDLIQTGGTFDPQTSTFTFDAPAGDQNIVSLPGGTSFYHLVIDRPSAFQYVNGSTVSV
                     LGDLTVASGLVSVDELRLKGDFNWYSDSGSGYLVGSRVVFDGGAPQTFRTTYSGSVGA
                     GDLWEFANTSPSGVTFDLSGGTVPYFKDITIAAGTKVTVAAGEIVLGGNATSRWANYG
                     TFDGSGSTVSFTSPGTVELLAGAQFAHVKLETQSNVLASTAMAVSGNFTVLNGTFTAG
                     DHVHSITGHLNLGGRFDLGESTLTVTGNTTVSAAGELNLSSGVGNFTGPLTVSGVLRL
                     PAASPAAVLKPVGGVTLASGAKFEAASVGSSTPTVTAGSAYSFVVSGATVDVNGLILK
                     NVSNAGLTLSADTRVDSFSNVRFDAPAGTTALLLQHASGTFNFTNLWFNDPNIAANIS
                     APNHVGTNGSSITVSGYGVQAGPLFENDPNNVIHWSNFDNVGPTSSVTYPVNGGYVTA
                     LSALTGTAADTPETGSGFSKVEMALRRLSDGYWWNGSTDFDQGVPYFALTSAQDGAFD
                     SNSEIWTYPMPTLSAGASYYAVSRASDTWRAESPLSAGVTFYFDDGAPQTGLLRPDGA
                     YQGSALTTLSGTASDSGFGLNTVQVRVRRLAGLNPYWNAALQNFDDALNADSAWFSAS
                     STGAWETWQSTQSLVFLVTGTTYTVEAKSRDLAGNWDAITSTGTFMYDATPAFSVLLL
                     PGNDTRWRALPVVSGTAADLHSPLSGVGVQ"
BASE COUNT         1266 a         1989 c         2140 g         1439 t
ORIGIN      
        1 cacctggtca ttgacgggtc cacgctcacg gttcagcccg gcgacggtct tgttttgaac
       61 ggcaactgga cggagcaatc cggcggtgtt tttagtcccg ccgtcggttc caccgtggcg
      121 ttcaccggaa ccagtgtgat ctccatgttg cccggctcgt ctttctatca ccttttggtc
      181 gatggggcgg tggatgtgaa agacgccacg gatgtgacca ttcgggggaa cttgacgttg
      241 aattcgggcg tttttacggt gaccaactcg acggtttctg tggcggggca cattcccacc
      301 ggcgggggaa gtctcggcca cggagccaat tcgacgtacg tcttcaacgg cgcagccaac
      361 cagaccatcg cctgccccga tttcaggaac atcgtctctc aaaacaacgg aaacacggtc
      421 tatttcccgg acctttcgaa tgtttatggg gattttacct tgatgggcgg cggcatggcc
      481 gatttcaaca atagaaacgt ctcgttttat gggagtttcg agcggtatgg gacgccggtt
      541 aatttggcga ccgccacgtt cgtgggcggc agcaacgccc agttcaattg caacgatccg
      601 ttcttgtccg acctggtggt ggctaagaac agcggcgccg ccttgtcggc cggcgacggg
      661 atgaacctcg acggcgactt cctcgttcag ggcggcacgt tcgtgcccgg cgcctatgtc
      721 cattcgctgg cggggaattt catcgttacc ggcggtttcc tggagacgga cgaggggaaa
      781 tttgttttcg acggatccgg cgaccaggcg ttgtcttgga cgggcggcaa tctggttttg
      841 tgcgatgtgg acgtcaacgg gggggcgagt gtgacccaga cgggcggtgg tctgttgatc
      901 agcactctga ccgtgaacgc gggtggtttc tacaatggat ccaaccaggg gacccggatc
      961 gatgggggca tcaataattc cggcaatctg atcgggctta tcgccacgtt cgagtcaagc
     1021 aacacgaaag tcatcaattc gacgacgacc ccgttttctc tgacggcggt gcaggtgcag
     1081 gagggggttt tgcagtcgaa cgtggccttg acggttccgg gcggtttcgg cgtgagcgcg
     1141 gggagcacct ttacggcggt gaacagcacg ttcgtgcaca ccttgaccgg tctcagcaac
     1201 agcggaaagt tctccgtgac gggggggacg gtgacgttca acggcgccgg cacgaacgat
     1261 gttttcgccg gcgacaatac gttcagccac ctcctcctca acatgggggc cggcgggctg
     1321 cttcaggtcc tgagcggttc tttgaacgtg gacggcaact tcacgctggc cgggggaacg
     1381 ttcacgctga acgacgccac gcacaccttc gccggcaatt ttacccagat gctcggcacc
     1441 acgctgaccg ccaacgggag caccatcaaa ttcgacgggt cggaaacgaa gagtttttcg
     1501 gccatggggc cattttataa cgtggttgtg gcaaacacgg gaggcgacct ggcgttggag
     1561 ggagacgtgg acatcaacgg ttctctgatc atcgagtctc cgagcggaag catcgattgg
     1621 tcggatgccg gccgcacggt ccgtttggcg ggtgatttca tcgacaacaa caatttttcg
     1681 caggcggtcg attcaaagtt gatcatggac ggggatgcct gtcagcacgt gggcggctca
     1741 agcacgaata tgctcgtcaa cttcgagatc aattcgtcca gcaccgtgga aatcttgacg
     1801 gcgaaaacgt ttgaaaacgt cgccatcctt ggcggaacaa tgactttggc ggtttcaaat
     1861 atcaacgttc ggggagattg gaatcatacg ggcggcggct tcctgccgaa tggaagcacg
     1921 gtgacgttca cgggcggcgt cccgatgaat gggcagtcgc ggaccgtcca atccgtttcg
     1981 gggagcgcat tcaatcattt ttcggtcaac gcgacgacgg ccatcgccat aacgagcgat
     2041 ttgggcgtga ccggcaattt cacgctcctc ggcggcacgg tgaccctgga cgggtacggg
     2101 ctggaggtgg ggggcgattg gacggagagc gcggcggtcc ggttccggtc cgccggggga
     2161 acggtgacgt ttcgaggcgg cggcgcgcaa tctgtcacgc agtctccttc cagcgctttc
     2221 ggcacgttgg aacacgccaa caactccaac ctcgttgcga actctgattt gaatattgga
     2281 gggaacttct atccgaacac cggttcgttc gacaccaacg gattcgaggt gcgcttaaaa
     2341 ggcgatttcc tgacgccgtc ggggacgttt aaccccagcg ggacatgggt ttttcagggc
     2401 agcggagcgc agaatgtcgc gcaggacgtg ttcgacaacg tcgatattca aaccggcggc
     2461 acccttgttt tcacgaaccc caacggattg aagaccaatg atgcgggcgg cacggggcat
     2521 ttgaccatcg ggcccgcggc gacggtcggg ttcggcggca acgacctgga ggtcgccgga
     2581 aatatgctca gttccggcac gatctcaggg gtggggctca ccaccttttc gggggcggcc
     2641 aaccaatccg tctggctgac gccttccagc tcccttggtt atacctacgt caacaagacc
     2701 ggtggtcttt tgaccgcgac caccggtctc acgttcttgg attcgctcaa catcgtcaac
     2761 ggcggtttcg atctggggct cggcacgcac gaggtctacg ggtttggata catcggcagc
     2821 ggcgggccca gcacgctgaa cgtttccagc gcggccgtct ttttccatga gaatgtttcg
     2881 gtgaccaacg gttccacctt gattttgccg gacagcgtct cgggggggaa gcttgctttg
     2941 gcttccggga agacccttca agtcgcttcg ggggagttcg tgtcccagtc cgtctacaat
     3001 atggtcggca gcacgcaaag cggagtgtac gtcaatttcg tcgttcagtc cggctccacc
     3061 atcaacgtgt cggggatgac gctgggacaa ttgggggaca acgggcttga aatccagagc
     3121 ggggcaaagg tggcggaatt gaacaacgtg aattaccggg agttcaaacc gggcagcaag
     3181 gggctgtatt tgaactgtga acttgtgtcg cccgccacgt tcaccgcgca tgattttgat
     3241 tcctccatca ccacgaacgt ctatctggcc gacaacatct tccccgccta catttatatg
     3301 cagggcgcca ccggtgtttc ttccggcccc gccagggaat acgatcccca caaccgcgtg
     3361 ttttggggag ttccgacgga gccgtccgcc cccgcggcct tggcggcttc cgccacgtcc
     3421 cttcagtggt cttggaccaa taattcgcag gattttgagg gcaccaagat atcgagcatg
     3481 tcttccggcg aaaattatct cctgtccgcg ggcacatcga ccaccagcca atacgaatac
     3541 atcctttcga caaacactct gtatacgcgg tacatttacg cctacagccc catcggcgaa
     3601 tcgttgcgcg tgagcaccca ggcctatacg tgggcggctc tcccgggggg aagcgcgtcc
     3661 actttcaccg cggtgggcgc caccattctg accgtcaatt ggacggacgg cggcaacccc
     3721 gccggaacca cctatatcgc ggacatctcc acgggaccgt tccccaacag ctacgccgac
     3781 aataaaagct ccacaacgct gaatacctac gcggtgttct ccggcttgga ccccatcacc
     3841 gtctattacg cccaggtgcg ggccgtcaac cacggcggcg tggagaccgg cgagttgccg
     3901 ctggggtcca cccagacgga gaacgcggtg atctggaccg gggccagtag cacctccagc
     3961 ctggcgtcgg acgtggacaa ctggctgagc aacgcccggc ccgtgaacga tgtgcgcgtg
     4021 atctttaacg ctcccggcag cgtccaggcc aactgtcagt gggatttgcc ggaggtcagc
     4081 atcatggaat tgcggctgga ggggacgttc aacggtaccg tgaccttgac ctcctccatg
     4141 accgccaccg gcgatgtggt tttggacgcc ggcacgttcg acagcaacaa ccggaacatg
     4201 tccgtgggcg gtgactgggg ggtgaacggg gctctctata actcgggcgg cgggggggcg
     4261 atcctcttca acggcgtctc gtcgcagacg gcggacctcg attacccctt gccgatcgtg
     4321 cgttctgaga acgccgcggg cctgacgttg ggttcgtccg ccggaacgat gcaggtgggg
     4381 ggcctcgggg tggaaccggg tgctcaattg gccttggctt ccgggaagac cctgttgatc
     4441 agcggaaatt tttcctccag cggcaccttc aataacaatg gttccacggt gtctttcggc
     4501 ggaaccggga gcgtctgggc tgtggcgggg acgacgttcg cgtgtgtttc ggtaaacgcc
     4561 gggactctgt cggcggccaa cgcgttggac gtcaacgggc acctgcgcat ttccggaggc
     4621 gtcttcaacg cgggcgcttt caatcacgcg ttggcgggtg atttgatcca gaccggcgga
     4681 actttcgatc cgcaaacgtc cacgttcact ttcgacgccc ccgcgggaga tcaaaacatc
     4741 gtttctctgc ccggcgggac cagtttttac cacttggtga tcgatcgtcc atcggctttc
     4801 caatatgtca acgggtcaac cgtgtcggtt ctgggggatt tgaccgtcgc cagcggtttg
     4861 gtatcggtcg acgagttacg gttgaaaggt gattttaatt ggtatagcga ttccggaagc
     4921 ggttatcttg tggggtcccg tgtggttttc gacggcggcg ccccgcagac gttccgcacg
     4981 acctattccg gaagcgtggg ggccggcgac ctttgggagt tcgccaacac ctccccgtcc
     5041 ggggtcactt ttgacttgtc gggcggcacc gtcccctatt tcaaggacat caccatcgct
     5101 gccgggacga aggtgacggt cgccgccggg gagatcgttt tggggggcaa tgcgaccagc
     5161 cgctgggcca actacgggac gttcgacggg agcggatcga ccgtttcttt tacgagcccg
     5221 ggaacggttg aactcctcgc cggcgcgcag tttgcgcacg tgaaactgga aacgcaatcg
     5281 aatgttttgg ccagcacggc catggccgtg agcggaaatt tcaccgtgtt gaacgggacc
     5341 tttacggcgg gcgaccacgt gcattccatc accggtcatt tgaatttggg aggtcggttc
     5401 gatttgggcg aaagcacgtt gaccgttacc gggaacacca cggtttctgc cgccggtgaa
     5461 ttgaacctca gttcgggggt gggcaacttc accggaccgt tgacggtgag cggtgtcctg
     5521 cgtctgccgg cggcgagtcc ggcggccgtg ttgaagcccg tggggggcgt gacgctcgct
     5581 tcgggggcca aattcgaggc ggcctcggtg ggttcctcga cacccacggt gacggcggga
     5641 tcggcctatt cttttgtggt ttccggcgcc acggtggacg tgaacggatt gatcctgaag
     5701 aacgtcagca acgccggatt gaccctttcc gccgataccc gcgtggattc gttcagcaac
     5761 gtgcggttcg atgcgccggc cggaaccacc gccttgctgc ttcaacatgc ttccggcacg
     5821 ttcaatttca ccaatctgtg gttcaatgac cccaacatcg ccgccaacat cagcgcgccc
     5881 aaccatgtcg gcaccaacgg cagcagcatc acggtgagcg gttacggtgt gcaggccggc
     5941 ccgcttttcg agaatgaccc caataacgtc atccactgga gcaacttcga taacgtcggg
     6001 ccgacgtctt ccgtgacata tcccgtgaac ggcggttatg tgaccgcgtt gtccgccctc
     6061 acgggcaccg ccgcggacac gccggaaacc ggatccggtt tcagcaaggt ggagatggcc
     6121 ctccggcgat tgagcgacgg ttactggtgg aacggatcga cggatttcga ccaaggcgtt
     6181 ccgtatttcg ccctcacgtc ggcccaggac ggcgcgttcg attcgaacag cgagatatgg
     6241 acgtatccca tgccgacgct gtcggccggc gcctcctatt acgcggtgag ccgcgctagt
     6301 gacacgtggc gggcggaaag cccgctgtcc gccggcgtca ctttttactt cgacgatggc
     6361 gcgccgcaga cgggactcct tcgccccgac ggcgcctacc aggggtctgc gttgacgacc
     6421 ctgtccggca cggcgtcgga ttcggggttc gggttgaaca ccgtccaggt gcgtgtgcga
     6481 cggctcgccg gcctgaatcc ctattggaac gccgctttgc agaatttcga cgacgcgctg
     6541 aacgcggatt cggcttggtt cagcgcctcc agcaccggcg catgggaaac gtggcaatcc
     6601 acccaatccc tcgtcttcct ggtgacaggg accacttata ccgtggaggc caagtcgcgg
     6661 gatttggcag gaaactggga tgccatcact tcgaccggca cgttcatgta tgacgcgacg
     6721 ccggcgttct cggttctact gctgccgggc aatgatacgc ggtggcgcgc gcttccagtc
     6781 gtttcgggga ccgcggccga tcttcattcg ccgctctccg gcgtcggcgt tcag
//
LOCUS       DAOTFD010000136         6751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8067_length_6751_cov_3.886649, whole genome shotgun sequence.
ACCESSION   DAOTFD010000136 DAOTFD010000000
VERSION     DAOTFD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_6751_cov_3.886649"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="P5079_08045"
     CDS             complement(<1..1681)
                     /locus_tag="P5079_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRY29969.1"
                     /translation="MRITFRIITPLLLVLTLVACVFAYFQVRHERDRQREELDRRARL
                     IAESLQETVEPLVAGGRRLELKRLVERFGNRERLAGVAVYDAEGKPLAVTTSLSVALA
                     ATPAPLVGAPGGLVDSSRFETLGNREMHVYVLPLSAQGNVSGSLVLLHDVSYIRTQLF
                     RIWRQAFLRVLIQMFLIALVTLLIIRWSVVDPITQMAEWMKKLRAGEEAEPSALPKAA
                     IFAPIAKEVTTFARHLSAAKMAAEEEARLRQAAESLWTPDRLKEHVRTKLGGKPLFVV
                     SNREPYMHVRRGNKIEYFVPAGGLVTALDPVLRSCGGTWIAHGAGDADWEGLDAQNRL
                     RVPPEDPLYTLKRVALTKEEEDGYYYGFSNDGLWPLCHIAHTRPIFRSEDWAHYQRAN
                     QKFARAVLEELENIEEPCILIQDYHFALLPRFLKVLRPDARIALFWHIPWPNPEAFGI
                     CPWQKELLFGMLGADLIGYHTQFHCNNFLDTVDRTLETRIDWEHFAVNKEGHTTLVKP
                     FPISVAFSDPGPSVPSEEDRKNLRETLLKQVGVKSAKHLGVGVDRLDYTKGI"
     gene            complement(1743..1913)
                     /locus_tag="P5079_08050"
     CDS             complement(1743..1913)
                     /locus_tag="P5079_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29970.1"
                     /translation="MSKEEKRGVKNTNEPTLPVPAAESGSVPVVRLMLAAFLCGGLLI
                     VLVVLKHVFKLF"
     gene            complement(2229..3482)
                     /gene="murA"
                     /locus_tag="P5079_08055"
     CDS             complement(2229..3482)
                     /gene="murA"
                     /locus_tag="P5079_08055"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY29971.1"
                     /translation="MDAFLIEGGRKLKGSVRISGSKNAALPCLFASLLTDDVCVIENV
                     PDLRDIRTAVALLQQLGKKVVRKNSRVEIGPARRLQHHASYDLVRRMRASVVVMGPLL
                     ARLGKARVSLPGGCSIGARPINFHLQAFKKLGAAIRVEEGYVEATAPVLRGRRIRFPF
                     PSVGATENALMAAVLAEGKTVLLNAAREPEIQDLAECLRSMGARIHGHGTGTIVIDGV
                     PSLHGTRHVVIPDRIEAGTFLAAAAITRGRLLLRRVNPAHLSAVLNALRRAGLRLEVG
                     RSEILAAWTKPLRPVSLKTRVYPGFPTDMQAQWMALMATVNGKSRITETIFENRFLHA
                     QELLRMGAAIDIRGAAAVIEGGRRLSGCPLMVSDLRAGAALVLAGLAAQGRTKVLRVY
                     HLDRGYERMERKLRAVGARIRRIAQ"
     gene            complement(3496..3972)
                     /locus_tag="P5079_08060"
     CDS             complement(3496..3972)
                     /locus_tag="P5079_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HRY29972.1"
                     /translation="MTLAKHLPGVRVTAVDISRKALRVAKKNAVQNGVAGKIRFVHGD
                     LLAPLRASAFLNAVVANLPYVARKDFSTLAPELRYEPRRALAGGPDGLSLIRRLIPQA
                     MEKLKPTGWLFLEVGHGQSRKVRAELRRAGFEDVAIKTDMAGIERFLAARKPKERV"
     gene            complement(3969..4370)
                     /locus_tag="P5079_08065"
     CDS             complement(3969..4370)
                     /locus_tag="P5079_08065"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29973.1"
                     /translation="MTTARPSTISEAYAESVRRLTERRVPDASTEAGELLATAIPCRR
                     LELPLLGREPMSRRQHASLRRLLRQRCRRKPLAYILGEWTFADVRLRLNRDVLIPRPE
                     TEELFEFVLKTLRSPRRATSPTSAPDPAAWP"
     gene            complement(4367..5428)
                     /gene="prfA"
                     /locus_tag="P5079_08070"
     CDS             complement(4367..5428)
                     /gene="prfA"
                     /locus_tag="P5079_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRY29974.1"
                     /translation="MNALYEELEKRYHDLQNALARSAEQGASSQAAAREFKKIEPMYQ
                     KAKTLRQISAEMDKLLPLKSSSDKEMQELAALELETLGTQKARLEQELDDFLHPADPA
                     DNRNVIIEIRAGTGGNEAALFAGDLFRMYTRYAAKRGFQAEVYSSHPTGLGGYKEIIF
                     GLQGDGAFRIFKHERGVHRVQRVPVTEASGRIHTSTATVAVLPEVEEIEVEINPADLR
                     IDTYRSSGAGGQHVNRTDSAVRITHLPTGTVVACQDERSQIKNRARAMSILRSKIYEQ
                     QREQAESERRDLRRSQVGTGERSEKIRTYNFPQDRITDHRANQNFHNLPAIMDGEMGE
                     IVDAMIAFERARKKAEAGT"
     gene            complement(5647..5838)
                     /gene="rpmE"
                     /locus_tag="P5079_08075"
                     /pseudo
     CDS             complement(5647..5838)
                     /gene="rpmE"
                     /locus_tag="P5079_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(5879..>6751)
                     /locus_tag="P5079_08080"
     CDS             complement(5879..>6751)
                     /locus_tag="P5079_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY29975.1"
                     /translation="EWTARPKVIVTADYASVRESADGTGPQVSDVVAGCLLALKSETG
                     DQYEVAFPDARTGSVRKADAAPWDAYLTKAKPTQESVVATAKRFMGVPYFWGGTSAKA
                     LDCSGFTKTVYFLNGVQLPRDASQQVYVGDSLDTSSEIDLQPGDLLFIPDAHLRFTDY
                     DFPVSWLRGKRFISSGYGMRRHPIFRYRGFHKGWDMPRPYGYPVKAAREGRVVFAGWR
                     GAYGRLIIIKHPGGAVTWYGHLSQMKVSAGDRVKKSQLIGKVGSSGNATGPHLHFEVR
                     DRFGNSLNPRKFLF"
BASE COUNT         1183 a         2106 c         2136 g         1326 t
ORIGIN      
        1 taatgccttt ggtgtagtcc aggcggtcca cgccgacccc gagatgtttg gcggacttga
       61 ccccgacttg cttcaggagc gtctcgcgga ggttcttccg gtcttcctcg gagggcacgc
      121 tcggccccgg atcggaaaag gcgacgctga tggggaacgg tttcaccagc gtggtgtggc
      181 cttccttgtt cacggcgaaa tgttcccaat cgatgcgcgt ttccagagtc cggtccacgg
      241 tgtccagaaa attgttgcag tggaactgcg tgtgatagcc gatgaggtcc gcccccaaca
      301 tcccgaaaag gagctctttt tgccagggac agatgccgaa ggcctcggga ttgggccaag
      361 ggatgtgcca gaacagggcg atccgcgcgt ccggacggag caccttcaag aaacggggca
      421 aaagggcgaa gtgatagtct tggatcagga tgcagggctc ttcgatgttt tccagttcct
      481 ctaggacggc gcgggcgaat ttttggttgg ccctttgata atgggcccag tcttccgatc
      541 ggaagatggg gcgggtgtgg gcgatgtgac agaggggcca aagtccgtcg ttggaaaatc
      601 cgtaatagta accgtcttcc tcctccttgg tgagggccac gcgtttcagc gtatagagcg
      661 gatcttccgg gggaacccga agccggttct gcgcgtccaa tccctcccag tcggcgtccc
      721 ccgccccgtg ggcgatccat gtcccgccgc aggagcggag gaccgggtcc aacgccgtca
      781 ccaatcctcc ggccgggacg aaatattcta ttttgttgcc ccgccggaca tgcatgtagg
      841 gttccctgtt ggaaacgacg aaaagcggtt tccccccgag tttggtccgg acatgttcct
      901 tgagccggtc gggggtccaa agagattccg ccgcctgcct caaccgggct tcttcttccg
      961 ccgccatctt ggccgccgag aggtggcggg cgaaggtcgt cacctccttg gcgatggggg
     1021 cgaaaatggc cgccttcggc aaggccgacg gttcggcctc ttccccggcg cggagtttct
     1081 tcatccattc cgccatctgg gtgatgggat cgacgacgct ccagcggatg atgagcaagg
     1141 tgaccagggc gatcaggaac atctggatca gcacgcgcaa gaaggcttgc cgccatatgc
     1201 ggaacaattg cgtccgtata taggagacgt cgtgcaacaa gacgagcgac cccgaaacgt
     1261 tcccttgggc gctcaacggt aggacgtaca cgtgcatctc ccggttcccc agggtttcga
     1321 atcggctgga atccacgagt ccgccgggcg ctccgacgag gggcgccggg gtggccgcca
     1381 aggcgacgga aaggctcgtg gtcacggcga gcggtttccc ttcggcgtcg tagaccgcca
     1441 cccccgccaa ccgttcgcgg ttcccaaacc gttcgacgag gcgcttcaat tcgaggcggc
     1501 ggccgccggc gacgagaggt tcgacggttt cctgcagact ttccgcgatg agccgcgccc
     1561 gccggtccaa ttcctcccgt tgccggtccc tttcgtgacg cacctggaaa taggcgaaca
     1621 cgcacgccac aagagtcagc acgaggagta acggagtgat gatccggaac gtaatgcgca
     1681 tcaggtcacc tcatcgccga agggaacgaa cgtggacttc tgaaatgatc ctgctccggc
     1741 cgctaaaaca acttgaagac gtgttttaga acgaccagga caatcaacaa tccaccgcac
     1801 aggaatgccg ccaacatcaa ccgcaccacg ggcaccgaac ccgactcggc cgccggaaca
     1861 ggcaatgtcg gttcgttcgt gttcttgacg ccccgcttct cttctttgga cataacgccc
     1921 cctgtgtttt tgaaacggcc catcggccgt tccgtcgttt acgaagagga ggcgtcctct
     1981 gttttaaacg ataacgccac cccgacgttg tcacgggagc ggaaaaaaac aaaaaaccgc
     2041 ccccccgact ctgcgggatg gcggcccgac catccaaagt cctccgggaa atacttccgg
     2101 ttcccggacc ccttgtgatg ttactgtcta aagtatatcc cctgcggagc ggggcgacaa
     2161 tccctttttc ggtttcgtat gggcgggtgg cgggcgatcc gcggagagcg cggggggcga
     2221 gaaacgcgtt attgggcgat tcggcggatg cgggcgccga cggcccggag tttgcgttcc
     2281 atccgttcgt agccgcggtc caaatgatag acgcggagga ctttcgtccg gccctgcgcg
     2341 gccagccccg ccagcaccag cgccgccccc gcccgcaggt cggagaccat caggggacag
     2401 cccgaaagac ggcgcccccc ctcgatcacg gcggcggcgc cccggatatc gatggccgcc
     2461 cccatgcgca ggagttcctg ggcgtgcagg aagcggtttt caaagattgt ttccgtgatg
     2521 cggcttttgc cgtttaccgt ggccatgagc gccatccatt gcgcctgcat atccgtcggg
     2581 aaacccggat agacgcgggt cttcagagag accggccgca ggggtttcgt ccaagcggcc
     2641 agaatctccg aacggcccac ctccaaccgc agcccggccc gccggagagc gttcagaaca
     2701 gcggacaaat gggcggggtt cacccggcgc aaaaggagac gtccccgggt gatggccgcc
     2761 gccgccagaa acgtgccggc ctcgatgcgg tccggtatca cgacatgccg cgtgccgtgc
     2821 agggaaggaa ccccgtcgat gacgatggtc cccgtgccgt gaccgtggat ccgggcgccc
     2881 atggagcgca ggcattcggc cagatcctgt atctccggtt cccgggccgc gttgagcagg
     2941 acggtcttcc cctcggcgag gacggccgcc atcagagcgt tctccgtcgc tcccacgctg
     3001 gggaaaggga atcggatccg ccggccgcgg aggaccggcg ccgtcgcttc cacataccct
     3061 tcttccacgc gaatggcggc gcccagtttt ttgaacgcct gcaggtggaa attgatgggg
     3121 cgggcgccga tggaacagcc tcccggcagg gacacccggg ccttgcccaa tcgcgccagc
     3181 agcggtccca tcaccaccac gctcgcccgc atgcgccgaa ccaggtcgta ggaggcgtga
     3241 tgttggagcc gccgggccgg gccgatctcg acgcgtgaat tctttcggac gacttttttc
     3301 cccagttgct gaagcagggc caccgcggtg cggatgtccc gcaggtccgg cacgttctcg
     3361 atcacgcaga cgtcgtcggt caacaacgag gcgaagaggc acggcagggc ggcgtttttg
     3421 gaaccggaaa tgcgcactga ccccttgagc ttccgccccc cttctatcag gaacgcgtcc
     3481 agtttcagca tgtttttaaa ccctttcctt cggttttcgg gcggccagga accgttcgat
     3541 tcccgccatg tccgttttaa tcgcgacgtc ctcaaatccg gctcgacgca gttcggcgcg
     3601 cactttccgg gactggccgt ggcccacctc cagaaagagc cagccggtcg gtttcagttt
     3661 ttccatcgcc tgtgggatga gacggcggat gagggagagc ccgtccggcc cgccggccaa
     3721 cgcccgtcgc ggttcataac ggagttccgg cgccaaggtc gaaaaatctt tccgggccac
     3781 gtacggcagg ttggccacca ccgcgttcag gaaggcgctc gcgcgcagcg gggccaataa
     3841 atcgccgtgc acgaagcgga tctttcccgc cacgccgttt tggacggcgt tcttcttggc
     3901 cacccggagg gctttccggg agatgtccac ggcagtcacg cggacgcccg gaaggtgttt
     3961 cgccagcgtc acggccaagc agccggatcc ggtgccgatg tcggcgacgt ggcgcggcgc
     4021 ggagacctca gcgttttcag aacgaactcg aaaagctctt ccgtctccgg cctcgggatg
     4081 agcacgtccc ggttgagccg gagccggacg tccgcgaaag tccattcgcc gaggatatag
     4141 gccagcggtt tccggcgaca gcgttggcgc agcagacgac ggagcgaggc gtgttggcgg
     4201 cgactcatcg gttcccggcc gagcaggggc agctccagcc gacggcaagg gatcgccgtg
     4261 gcgagcagtt cccccgcctc cgtggaggcg tcgggaaccc gtcgttccgt caaccggcgc
     4321 acggactcgg cgtaggcctc cgagatcgtc gacggccggg ccgtggtcac gtcccggcct
     4381 cggccttctt gcgggcccgt tcgaacgcga tcatcgcgtc cacgatctcc cccatttctc
     4441 cgtccatgat cgcgggaagg ttgtggaaat tctggttggc gcggtggtcg gtgatccggt
     4501 cctggggaaa attgtaggtg cgtatctttt cactccgttc gcccgtgccc acctgggagc
     4561 gccgaaggtc ccggcgctcg ctctcggcct gttcccgctg ttgttcatag atcttggatc
     4621 gcaggatgct catggcccgg gcgcggttct ttatctggct gcgctcgtcc tggcaggcca
     4681 ccacggtgcc ggtgggcagg tgggtgatcc gcaccgccga gtccgtgcgg ttcacgtgct
     4741 gtccgccggc gccgctggag cggtaggtgt cgatacgcag gtcggcgggg ttgatctcca
     4801 cttcgatctc ctccacttcc ggcaacaccg ccaccgtggc ggtggaggta tggatgcggc
     4861 cgctggcttc cgtcaccggc acgcgctgca cgcggtgcac cccccgttcg tgtttgaaga
     4921 tgcggaaagc cccgtccccc tgaaggccga aaatgatttc tttgtacccg cccagtcccg
     4981 tgggatggct ggaatagacc tccgcctgga agccgcgttt ggcggcgtag cgggtataca
     5041 tgcggaaaag gtcccccgcg aacagggcgg cctcgttgcc gccggtgccg gcgcggatct
     5101 cgatgatgac gttgcggttg tcggccgggt ccgccggatg aaggaaatcg tcgagctcct
     5161 gttccagccg ggccttctgg gttccgaggg tctccagttc cagcgcggcc agttcctgca
     5221 tctctttgtc cgacgatgat ttcagcggca ggagtttgtc catctccgcc gagatctgtc
     5281 gcaaggtttt ggctttctga tacatgggtt cgatcttttt gaattccctg gccgcggcct
     5341 gggacgacgc cccttgttcg gccgaacggg ccagggcgtt ctgaaggtcg tggtatcgtt
     5401 tttccagttc ttcgtagagt gcgttcattg tcggggccgg tcaaaaaaaa tacgccccgg
     5461 agcgaggcgt atttttttca aaagaggaag gcgctagcgg ccggcgggtt tggcggattt
     5521 gcttcccttg ggcggcggcg cggtgacgcg cggcgcgttg cggaggatgc gtttcgtcgt
     5581 cttcgtgacc ttgggcgccg cctgtttggt ggcggctttg cgaaccacgg tcttccctcc
     5641 ggttttggca tagcgttttt caaaacgttc catacgtccg gcggtgtcca acagtttctg
     5701 gcggccggta tagaagggat ggcagttgga acagatgtcc agccgcattt gcggccgggt
     5761 ggaccgggtc acaaaggtgt tgccgcaggc gcaggtgacc gtcgctattt gatattgggg
     5821 atgaatgcct tctttcataa gagtcctttc aatggggaac gcgttccgta agggtatatt
     5881 aaaacaaaaa ttttctcgga tttaaagaat ttccgaagcg gtcccggacc tcgaaatgga
     5941 ggtgcggccc ggtggcgttg ccgctgcttc ccactttgcc gatcaattgg ctctttttca
     6001 cccggtcccc cgccgacacc ttcatctggg agaggtggcc gtaccacgtc acggccccgc
     6061 ccggatgttt gatgatgatc agccggccgt aggccccccg ccagcccgca aaaacgaccc
     6121 gcccttcccg ggccgccttc accggatatc cgtagggccg gggcatgtcc caccctttgt
     6181 ggaaaccgcg gtagcggaaa atggggtggc ggcgcatgcc gtagcccgac gagatgaagc
     6241 gtttcccccg cagccacgac accgggaagt cgtaatccgt gaaacgcaga tgggcgtcgg
     6301 ggatgaacag cagatccccc ggctgaagat cgatctcgct ggaagtgtcc agcgaatcgc
     6361 cgacgtacac ctgctgactc gcatcgcgcg ggagctggac cccgttaaga aagtagacgg
     6421 tctttgtgaa acccgaacag tccagggctt ttgccgatgt tccgccccag aagtatggca
     6481 cgcccatgaa gcgcttcgcc gtggccacaa cggattcctg tgtcggcttc gcctttgtca
     6541 ggtacgcatc ccacggcgca gcgtcggcct tgcgcacaga gcccgtgcgc gcatccggga
     6601 atgccacttc atactgatcg cccgtttcgc ttttcagggc aagcaggcac ccggcaacga
     6661 catcgctcac ctgcggaccc gtcccgtccg ctgattcccg caccgatgca tagtcggcag
     6721 tgacgatgac cttcgggcgg gcggtccatt c
//
LOCUS       DAOTFD010000137         6725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8110_length_6725_cov_5.107346, whole genome shotgun sequence.
ACCESSION   DAOTFD010000137 DAOTFD010000000
VERSION     DAOTFD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_6725_cov_5.107346"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="P5079_08085"
     CDS             <1..1300
                     /locus_tag="P5079_08085"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRY29976.1"
                     /translation="QWKEITPAFMESYRNTGGFDIIQSGRTKIETPEQFDQTKKNLTG
                     NGLSALVVVGGDDSNTNAGLLAEYFKAQDIPLSVIGVPKTIDGDLKNEHVETSFGFDT
                     ATKIYAELAGNICRDVNSARKYWHFVRLMGRSASHITLEVGLKVQPNIVLVGEEVLAH
                     RMTLAQVVDGMADVVVRRAARGKNFGVALVPEGLIEFIPEMKELISALNDVLAENEAA
                     LAALGGFADKRDKVLTLLPEALGGLLRSLPPGIQSQLMLDRDPHGNVTVSQIETERLL
                     AEMLQTRLAEMKKAGAYAGKFAAITHFFGYEGRCGAPSNFDANYCYALGFNASLLALN
                     GCSGYLSSVRGLTGPASRWDCGGIPLTMMMNIERRKGKEKPVIQKALVKLEGAPFKAL
                     RKNREDWAIHDRYLFPGPIQFFGPSSVTDLPTATLRLEQG"
     gene            1323..1736
                     /locus_tag="P5079_08090"
     CDS             1323..1736
                     /locus_tag="P5079_08090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY29977.1"
                     /translation="MHELTYRVSYADTDKMGVVYYSNYLVLFERGRTELLREAGARYR
                     DVEENLKTYLPAVEAHLEFQAPARYDDLLVILTRVSELGKASVTFQYEVLSSEPRRLL
                     VKGHTRHLFVNERWKPIRVPEQLKTLLSHHTAPFS"
     gene            1738..3678
                     /locus_tag="P5079_08095"
     CDS             1738..3678
                     /locus_tag="P5079_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29978.1"
                     /translation="MARPARNVLEQPNPGAPFLVWLTAALFVALALVPDEKLVRLKLL
                     VFELGLFSFVLLWALRGVWRARLEISLPLPLWGVAAWAAYVGLRAACSDYPALAEGEL
                     RRVLLCLAAFWASAQAALQPRGREILWRAWGVGTALVSLYGVLQRSGGVGRLAVPSME
                     RVMGSFGNPIFFGAYLAVSIPLLWALCRETKGRERIFWSVSLGLALAALYLTRTRAAW
                     IGLAVGLGSAVFLSWKRKKYAAVVLLFLVAAGGLFVSRTKSVWLRDQAHLLIWRDTLK
                     LWLAHPAAGVGPGEFHIHFPSVAGPDLKAKWPPNQFIVNYAHNEFLQVLSETGVLGLG
                     LWLGLFALFYRPFFSRRDSGKDGTPVPPKETALFCAVTAGLVQSFFSVDPRFSVSAGT
                     LFFLLGQSVPPAGVPVKETSWNSTLVRKLAATAVLLWVSGVVAVERPGAGWGVNLGGF
                     YQLRNAAGRPKGSVFPEEMRAGFLTRLLHPYAAQRRRASAPDFFDERLLHPEQTIADL
                     EALGRRYPSEPAVFEKLGYAYAKEMRTRGADGRPVLRPEMADKAVKAYQRAAELDPAR
                     AGVYNNLGNIFYTRGDQRRAMEQWAKAVQADPNQLDVRLNLGKVYYLNGRLKEAVEQF
                     RKVLELQPGNDEATVYLKKMVE"
     gene            3715..4152
                     /locus_tag="P5079_08100"
     CDS             3715..4152
                     /locus_tag="P5079_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29979.1"
                     /translation="MLWPVLLWILGLFLVAWVFYNAWENINMPAGTSSTGRRLRALSG
                     DRVRSLGELLIANWLFKRKIPFAYDKAVSFSLREPPLRPDFLLDDRHCFIEFLGKEAD
                     RAARDQKEKLYSEKGWKVVWVTPEDLDRLNEVLSPLTSQPAGV"
     gene            4145..5317
                     /gene="rlmD"
                     /locus_tag="P5079_08105"
     CDS             4145..5317
                     /gene="rlmD"
                     /locus_tag="P5079_08105"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRY29980.1"
                     /translation="MSDAAGRLCRHFGVCGGCAFQDKPYGEQLAAKEQIVRHVVEPFR
                     PERFLPIRPSPQAYFYRNKMEFAFGGVLGGPALLGLREKGRFNRIVSLEECLLLSPEV
                     PALLESVRRWADEEKVPTYHLRAHKGFLRYLAVREGKNTGERMVHLVTAPGELPRDSF
                     LAALKRSGVPVTSAVWSVNEALSDVAYGKVSAVLSGSGSITEELLGRRFRISPTAFFQ
                     TNTRGAEILYKIVAEALGGGAETLFDFYCGSGAIGLCCADAGRRVIGVEVHAPAVEDA
                     RLNAREQGADRAEFHAADAAAFVRLPEWMSLWGSPSTAAVFDPPRTGLSAPVRELLVT
                     RPLERWIYVSCNPEALARDLPALAGTYRLESVQPVDLFPHTEHVETVLLFHRRPPA"
     gene            5347..5919
                     /locus_tag="P5079_08110"
     CDS             5347..5919
                     /locus_tag="P5079_08110"
                     /inference="COORDINATES: protein motif:HMM:NF020398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HRY29981.1"
                     /translation="MKSQNDKALKEWAVVERALREGWQVFVLAGEGVAEESGAPAWTE
                     EEFFICKTYDRQTPEALKEKFHEWPYDAERLRAPQGELNIESYARVDSLVKVADLEAL
                     KRVLPHTVWSESHLRKSFESRRPLYLAFLRCYRTPHPLRVSPPTEQEAPSSPWLTLKE
                     PLSPTNFYPVFSPEVFAEKKKKVLDSLNGA"
     gene            5909..>6725
                     /locus_tag="P5079_08115"
     CDS             5909..>6725
                     /locus_tag="P5079_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY29982.1"
                     /translation="MALDEKLLSQLNPPQRQAVLHGEGPLLILAGAGSGKTRVITYRI
                     AQLLSEGVPAWNILAVTFTNKAAAEMRRRVDDLTGGLGRDVWISTFHSFCAQFLRAEG
                     RAVGLEPHYVIYDDADQRMLVKECLRELNLDETKHKPGQIVELISRAKDDLLDADSYA
                     IHALAQNDPFRQRVATVYAVYQQKMAKANALDFGDLILRTVLALRDNEPLREKYQRRL
                     RHVLVDEYQDTNHAQYLLTKYLVAPPKNICVVGDDDQSIYSCRGANVRNILEFE"
BASE COUNT         1175 a         2091 c         2143 g         1316 t
ORIGIN      
        1 ccaatggaag gagatcaccc ccgccttcat ggagtcctac cgcaataccg gcgggttcga
       61 catcatccag tcgggacgga ccaaaattga gacgcccgaa cagttcgatc aaaccaagaa
      121 aaatctgacc ggcaacgggc tgtccgccct ggtggtggtg ggcggggacg actccaacac
      181 caacgccggc ctcctggccg agtatttcaa ggcgcaggac atcccgttga gcgtcatcgg
      241 cgtgcccaag accatcgacg gcgatttgaa gaacgagcat gtggagacct ctttcggttt
      301 cgacaccgcc accaagatct acgcggaact ggcggggaac atctgccgcg acgtgaattc
      361 ggcccggaag tattggcact tcgtccgcct gatgggacgg agcgcctccc acatcaccct
      421 cgaggtgggc ctgaaggtcc agccgaacat cgtgctggtg ggcgaagagg tgctggctca
      481 tcggatgacg ctggcccagg tggtggacgg catggcggac gtcgtcgtgc gacgcgccgc
      541 ccgggggaaa aatttcggag tggcgctcgt ccccgaaggg ttgatcgagt tcatccccga
      601 gatgaaggaa ctcatctcgg ccttgaacga cgtgctggcc gaaaacgagg cggccctcgc
      661 cgccctcggc ggattcgcgg acaagaggga caaggtgctg accttgttgc ccgaggccct
      721 cggcggcctc ctgcgctcgt tgccccccgg catccagtcc cagttgatgc tggaccgcga
      781 cccgcacggc aacgtcaccg tgtcccagat cgaaacggag cggctgctgg cggagatgtt
      841 gcagacgcgt ttggccgaga tgaaaaaggc gggagcctat gccgggaagt tcgcggccat
      901 cacacatttc ttcggctacg agggccgctg cggcgcgccc tccaacttcg acgccaacta
      961 ctgctacgcc ctcggtttca acgcctccct gctcgccttg aacgggtgct ccggctatct
     1021 gtcgtcggtg cgaggcctca cgggtcccgc ctcccgctgg gactgcggcg ggatcccgct
     1081 gaccatgatg atgaacatcg aacgccgaaa agggaaagag aaaccggtga tccaaaaggc
     1141 cctggtgaag ctcgaagggg cgcccttcaa ggccctgcgg aagaaccggg aagactgggc
     1201 gatccacgac cggtacctgt tccccggccc catccagttc ttcggaccgt cgtcggtgac
     1261 ggatctgccg accgcgacgc tccggctgga acagggatag attttttttc ggacccttct
     1321 tcatgcatga gctgacctac cgcgtgtctt acgccgacac ggacaaaatg ggcgtggtct
     1381 actattccaa ctacctggtg cttttcgagc ggggccgcac cgaactcctg cgcgaggcgg
     1441 gggcccgcta tcgcgacgtg gaggagaatc tgaagaccta ccttccggcg gtggaggccc
     1501 atctggaatt ccaggcgccg gcccgatacg acgacctttt ggtcatcctc acccgggtat
     1561 cggaattggg caaggccagc gtcaccttcc aatacgaggt gctttcttcc gagccgcgcc
     1621 ggctcctggt gaaggggcac acccggcatc tttttgtgaa tgaacgctgg aaacccatcc
     1681 gcgttcccga acagcttaaa acgctccttt cccaccacac cgcgcccttc tcttgacatg
     1741 gcccgtccgg cccgaaacgt cctcgaacag ccgaaccccg gagccccctt cctggtttgg
     1801 ctgacggcgg ctcttttcgt cgccttggcc ctcgtgcccg acgaaaaact ggtccggctc
     1861 aaactcctgg tgttcgagct gggtttgttt tcttttgtgc tcctgtgggc tctccggggc
     1921 gtgtggcggg cgcggttgga aatctccctg ccgctgccgt tgtggggcgt ggcggcgtgg
     1981 gcggcttacg tcggtctccg cgccgcgtgc agcgattatc ccgcgctggc cgagggggaa
     2041 ctgcgccggg tcttgttgtg tctggccgcg ttctgggcgt cggctcaggc cgctttacag
     2101 ccgcgcggcc gggagattct ctggcgggcc tggggcgtgg gcacggccct cgtctcactg
     2161 tatggcgtgt tgcagcgcag cggcggggtc ggccgtctgg cggtaccgtc gatggaacgc
     2221 gtgatgggca gtttcggcaa ccctattttt ttcggcgcct atctggcggt ttccatcccg
     2281 ctgttgtggg cgttgtgccg cgagacaaaa gggcgggaaa ggatcttttg gtccgtgtcc
     2341 ctgggcctgg cgctggcggc gctctatttg acgcggaccc gcgcggcctg gatcggattg
     2401 gccgtcggcc tggggtcggc ggtctttttg agttggaaac gaaaaaagta tgcggccgtc
     2461 gttttgttgt tcctggtcgc cgccggcggt cttttcgtca gccggacgaa gtctgtctgg
     2521 ttgcgcgacc aggcccatct cctcatttgg agggacacgc tcaaattgtg gctcgcgcat
     2581 ccggcggccg gggtggggcc gggcgagttc cacattcatt tcccgtcggt ggccggcccc
     2641 gacctgaaag ccaaatggcc cccgaaccag ttcatcgtca actacgccca caacgagttc
     2701 ctgcaggttc tgtccgagac gggagtcctc gggctgggtc tgtggctcgg tctgttcgcc
     2761 ttgttctatc ggccgttttt ctcgcgacgg gacagcggca aggacgggac acccgttccg
     2821 cccaaggaga ccgcgctctt ctgcgccgtc accgccggct tggtccagtc ttttttttcc
     2881 gtggatccgc gtttttccgt cagcgccggc acgctctttt ttctgctggg ccaatcggtc
     2941 ccgcccgccg gcgttccggt gaaagagacg tcgtggaatt cgaccttggt gagaaaactg
     3001 gcggccaccg cggtcctttt gtgggtgtcg ggcgtggtgg ccgtcgagcg accgggggcc
     3061 gggtggggcg tgaacctggg gggtttttac cagctgcgga acgcggccgg gagaccgaaa
     3121 gggtccgttt tccctgaaga gatgcgggcc ggtttcctga cccgcctctt gcatccctac
     3181 gccgctcagc gtcgacgcgc gagcgccccg gattttttcg atgaacgtct tctccatccc
     3241 gaacagacca ttgccgatct ggaggcgttg ggccgacgct atccctcgga accggcggtt
     3301 tttgagaagc tcggctatgc ctacgccaaa gagatgcgta cccgaggggc cgacggccgg
     3361 cccgtgctgc gtccggagat ggccgacaag gcggtaaagg cctatcagcg ggcggccgaa
     3421 ttggatcccg cccgggccgg cgtctacaac aatctgggga acatatttta tacgcggggc
     3481 gaccaacgcc gcgccatgga acagtgggcg aaagcggtcc aggccgatcc caatcagttg
     3541 gacgtccgcc tgaacctggg gaaggtctat tatttgaacg ggcggctcaa agaggccgtc
     3601 gaacagtttc ggaaagtgct ggaacttcag cccggcaacg acgaggccac ggtttatctc
     3661 aagaagatgg tagaataatg aggatggaac gaacgaaaaa gaaggaacgt tgagatgctc
     3721 tggccggtgc tgttgtggat tttgggactg tttctcgtcg cgtgggtctt ttataacgcg
     3781 tgggagaaca tcaacatgcc cgccggaacg tcgtccacgg gccgccgcct ccgcgccctt
     3841 tccggcgacc gcgtgaggtc cctgggggaa ctcctcatcg ccaactggct tttcaaacgc
     3901 aagatcccgt tcgcctacga caaggcggtg tctttttccc tccgggagcc tccccttcgg
     3961 ccggatttcc tcctcgacga ccgccattgt ttcatcgaat ttttaggcaa ggaggcggac
     4021 cgggccgccc gcgatcaaaa agaaaaactg tattcggaaa agggatggaa ggtcgtttgg
     4081 gtgacgccgg aagatttgga ccgcctcaac gaggtgttgt ccccgctgac gtcccagccg
     4141 gccggtgtct gacgccgccg gccgcctctg ccgccatttc ggcgtctgcg gcgggtgcgc
     4201 ttttcaggac aagccttacg gcgagcaact cgccgccaag gaacaaatcg tccgtcacgt
     4261 ggtcgaaccg ttccgtccgg aacggttcct gccgatccgg ccctccccgc aagcgtattt
     4321 ttatcggaac aagatggagt tcgccttcgg gggcgtcctt ggcggtccgg cgttgttggg
     4381 cctgcgtgaa aaaggccggt tcaacaggat cgtcagcttg gaggaatgcc tcctgctgtc
     4441 gcccgaagtc ccggccctgc tggaatccgt gcgccgctgg gcggacgagg aaaaggtgcc
     4501 gacctatcac cttcgggcgc acaaaggttt tttacgctac ctggcggtgc gcgaggggaa
     4561 aaacaccggc gagcggatgg tccatctggt cacggcgccg ggcgaactgc cgcgcgattc
     4621 ttttttggcg gccctgaaac gttccggggt tcctgtcacc tcggcggtgt ggagcgtgaa
     4681 cgaggctctt tccgacgtgg cctatgggaa ggtctccgcg gtgttgtccg gttccggatc
     4741 catcacggag gagttgctgg ggcggcggtt ccgcatctcg ccgacggcgt tttttcagac
     4801 caatacccgc ggcgctgaaa tactttataa aatcgtggcg gaggccctgg gcggaggggc
     4861 ggaaacgttg ttcgactttt attgcgggtc cggggccatc gggctgtgtt gcgcggacgc
     4921 cggccggcgg gtcatcggcg tggaggtcca cgctcccgct gtggaggacg cccgcctcaa
     4981 cgcccgtgaa cagggcgccg accgggcgga gttccacgcc gccgacgccg ccgcgttcgt
     5041 ccgcctgccg gagtggatgt ccctctgggg aagcccctcg accgccgccg ttttcgatcc
     5101 gcctcgcacg gggctttccg ctccggtgcg ggaactcctc gtcacgcgcc cgttggaacg
     5161 ctggatctac gtctcgtgca atcccgaggc cttggcgagg gacctgcccg ccctcgccgg
     5221 gacgtatcga ttggagtcgg tccagccggt ggaccttttt ccgcataccg aacacgtgga
     5281 gacggtgctc cttttccatc gacgaccgcc cgcgtaacgg agaacaagag aatcaggagg
     5341 ttttttatga aatcgcagaa cgacaaggcc ctgaaagaat gggcggtggt cgaacgcgcc
     5401 ctccgggaag gatggcaggt gttcgtgttg gccggcgagg gcgtcgccga ggagtccggg
     5461 gcgcccgctt ggacggagga ggaattcttc atctgcaaaa cctacgaccg gcagacgccg
     5521 gaggcgctga aggagaagtt ccacgaatgg ccgtacgacg ccgaacggct ccgcgctccc
     5581 cagggggaat tgaacatcga atcctacgcc cgggtggatt ccctggtgaa agtggccgat
     5641 cttgaggcgc tcaagagggt cctgccgcat acggtgtggt cggaatcgca tctgcggaaa
     5701 agttttgagt cgcgaagacc tctgtacctg gcgtttttgc ggtgctatcg cacgccccat
     5761 cccctgcgcg tgtctccgcc gaccgaacaa gaggcgccgt cgtctccctg gttgacgctg
     5821 aaggaacctc tctcccccac gaacttctat ccggtgttct cgccggaggt gttcgcggag
     5881 aaaaagaaaa aggtgctgga ttcgttgaat ggcgcttgat gaaaaacttc tgtcccagtt
     5941 gaacccgccc cagcggcagg cggtgctgca cggcgaaggc ccgctgttga tcctagccgg
     6001 cgccggatcc ggaaagaccc gggtcatcac ctaccgcatc gcccagctcc tgtcggaggg
     6061 ggtgccggcg tggaacatcc tggccgtcac gttcaccaac aaggcggcgg cggagatgcg
     6121 ccgccgggtg gacgacctca ccggcggatt gggccgcgat gtgtggatat ccacgttcca
     6181 ctccttctgc gcccagttcc tccgcgccga aggccgggcc gtcggcctgg aaccccacta
     6241 cgtgatctac gacgacgccg atcagcgcat gctggtgaag gaatgcctgc gcgaactgaa
     6301 cctggacgag accaagcaca aaccgggcca gatcgtcgag ctcatctccc gcgccaagga
     6361 cgacctcctc gacgccgact cctacgccat ccacgccctc gcccagaacg acccgttccg
     6421 ccagagggtg gccaccgtgt atgcggtcta tcagcagaaa atggccaaag ccaacgccct
     6481 ggatttcggc gacctcatcc tccgcaccgt tctggccctg cgcgacaacg aaccgctgcg
     6541 ggaaaagtac cagcgtcgtc tgcggcacgt gttggtggac gaataccagg acaccaacca
     6601 cgcccaatac ctcctcacca aatacctcgt cgcgccgccc aagaacatct gcgtggtggg
     6661 cgacgacgac cagtccattt attcctgccg cggcgccaat gtgcgcaaca tcctggaatt
     6721 cgagc
//
LOCUS       DAOTFD010000138         6691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8171_length_6691_cov_3.927969, whole genome shotgun sequence.
ACCESSION   DAOTFD010000138 DAOTFD010000000
VERSION     DAOTFD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8171_length_6691_cov_3.927969"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2185
                     /gene="lon"
                     /locus_tag="P5079_08120"
     CDS             <1..>2185
                     /gene="lon"
                     /locus_tag="P5079_08120"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY29983.1"
                     /translation="LERAAGTVELENRSEVTPQDIKTAHAALQDQGAAAGVAIEERYR
                     AMIEEMPDQVRNTALKQLERLRRLSPHDAEREVVLNYLDNLTQISWTVRTEDETDLGK
                     VQNALEASHYGMKEAKEQILEYIVERTLAAKKQGKIICWTGPPGVGKSTLAETVAAAM
                     GRKFVRVSLGGVRDEAEIRGHRRTYIGAMPGLVVKALQQAGSMNPVLLLDEVDKMSSD
                     FRGDPVAALMQVLDAQQNHDFQDHYMEVGVDLSETMFILTANDLSKIPAPLRDRMEVI
                     QLPGYTPDEKIAIGRDYALPRLMKDLGITPEKASIPDTDALIRTVVENYTREAGARQL
                     ESRLRKILVKALHEWSLRKDAPISLTPEKVLRYLGQPLETDVMRDEDTVGEVMGLAWT
                     PAGGDILFVQASVYETGGRGPLSVDITGNLQGVMTESAGLALNVARRRLAQMGWNAEQ
                     LIGKRIFIHVPQGGIPKDGPSAGVTFVTAILSELTGRPVRRDVAMTGEVDAKGNVLAI
                     GGLKEKLLAARKAGVKTVFVPAANKDALEGVFKSAPELRGIVEQNGRHTLLVPKTSST
                     KDWAGLLKQIKAKGPGIAVTEKKIGSWEGVELAGTPDQIRNLLVHFPQIAPGPLRYVL
                     AEKIDDVLDQALITAEPPADSDQPRSPPAAGKRSERGTATVDALLVLSGLLLLGSLTL
                     IAVGSFVPWLVGFLLDTTLSLPLLQWSLVVSSPFLLVTAGIAVLI"
     assembly_gap    2186..2195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2196..>6691
                     /locus_tag="P5079_08125"
     CDS             <2196..>6691
                     /locus_tag="P5079_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29984.1"
                     /translation="TVYLAHTLFAVMKKRRALGWLLAFFLGGQLIVDKLCDWLTSYLL
                     PNNFTGGKFQNSLSPPFPIPFPKAQPDVDAPIADVREFSARAGRNAFWENETERAITK
                     NGGYAQIVDHDNGRPVTRIEKLLRSVKAEGGGVARVWLFRDMRDMDELFETVNGVPVR
                     MRPEVFEVTRAMLDAQRRTGVKILPSLFDFHILNSVRSETRGTPSGLLTTPVGEHPEF
                     FTAIGRDRMLDLFRPWMKEFAAQFDLIDLFNEPDNVRPALTSGVAYNPHVMLFLQTFA
                     ALAHEHGGKITVGVDKMGRLHRYQMIIRPDLGDVYSVHLYGYKPGQLPPAGSLGVGTA
                     PIWITEIGASTPEEVAAFTREAKEKGYRGILFWPQDGFTHEGYGQTLRDMEQTESVAG
                     PSLTDQPGVSASPPLSLGTNLFGRLLAAIRLMGETRPPATRAPPTRTAERGSARITAL
                     LFLSGLTLAAFILAGHLPASFADLGHISLSPLTVSASGILLGGFLVLLVRFLRMTKPD
                     AAYITPRAGRNYWKHKFLALAFGFFLIFGPFSAGVAAPTPTPSSDNAAVAAQLEKLPR
                     LSAKPQVSLGDINEKLTALSKISMPTIRGNLMFLDHVVDVLQALSQDGALQKKLIKTD
                     PAHLGRIFYSMERMSAEWDKQIFIHVHQAPAAEKDEARRKARQILVDDFYAKHFQPLL
                     SFWEQWHQENDKYHHLSCLLGRSLAYAVNMGAPYADSLTDAQLQKLLQDPQVVMGLYW
                     NLDTPKHNDLVNPAASPTHPFTQALRRQIGPEWQEFLLKAIPATNQQVAAITNGETDI
                     MDYGNDIFALLEEAEKLPKDRPLAELFLKKAVLNVSLTDFLRRRMTPEQWNDPQKRVQ
                     LINNARGLLAMMSAFVGPEYVAKTGEVSAFASYPAITFGSSHRYAIDLSRIGHEAVHQ
                     LDRAMWFILNDPSMNDYWAWGFGDHTLNIPSRFDRYLLKNFYPPPLTPLVDVPLLASE
                     METSHVLEFVARKNNKTIADVLADVRKRSPEAVAQDLLRWVRDENIAEVEYVVNQQVG
                     ISAAYGRRANSDFTDPSSVFEIPKVVKRTTTLKSLYQHRLQNILAGMRLAQESSSPQE
                     AYLAIMRKIYEQPKNMTYAEFVNAIGTANTQDVRDLLSKHFKDRNYTYEKLIKDVASR
                     THPLGKKVVEIGMGRDTVGVEGQIDRSGAGYMLAGLIYAIAGNDHAKAEELQRNLSHF
                     ETLLHAFDGLELPKVSANNVSPDPEQPFYRTAPPYVAGQAAHLTAPAHGVSASSYKGR
                     SQTAQVRRLYTPQKVPSRHAQTKVPSSWNAALVVGGLVLAFGLFKFGWPALFSLLLMP
                     GRLWYVLMARPGWPTLTPSMQAEFKNKLQATLGSRFGRVEIFDFPGETRSTLSLLKSP
                     YAQLTPLAYVTPSAAEGEGDILYLHRSVAFALSDYNDKTSTPNVLLRSLTRAVAETVL
                     LHELSQHGRRESAGILAQFGWGRKLRPFVSPKLFLARLIVNLADVPQLVWARVGALRP
                     IKTP"
BASE COUNT         1358 a         2285 c         1879 g         1159 t
ORIGIN      
        1 tattggaacg cgccgccggg acggtggaac tcgaaaaccg gtccgaggtg acgccccaag
       61 acataaaaac ggcccacgcc gccctgcaag accagggggc cgccgccggc gtggcgatcg
      121 aagagcgcta ccgcgccatg atcgaagaga tgccggacca ggtccgcaac accgccctca
      181 agcaactgga acgcctgagg cgtctttcac cccacgacgc cgaaagggaa gtggtgctga
      241 actatctcga caacctcacg cagatctcct ggaccgtgcg gaccgaggac gagacggact
      301 tgggcaaggt gcaaaacgcg ctggaagcca gccattacgg gatgaaagaa gccaaggaac
      361 aaattttgga atacatcgtc gaacgaaccc tggccgcgaa gaaacagggg aaaatcatct
      421 gctggaccgg gccgcccggc gtcggcaaaa gcacgctggc ggaaacggtg gccgccgcca
      481 tgggacgcaa attcgtccgc gtcagtttgg gcggggtccg cgacgaggcc gagatccgcg
      541 ggcaccgccg cacctacatc ggcgccatgc cgggactggt cgtcaaggcc ctgcaacagg
      601 ccggcagcat gaaccccgtc ctcctcttgg acgaagtcga caagatgtcg tccgatttcc
      661 gaggcgaccc cgtcgccgcc ctcatgcagg tgctcgacgc ccaacagaac cacgatttcc
      721 aagaccatta tatggaagtg ggcgtggacc tctcggaaac gatgttcatc ctcaccgcca
      781 acgatctctc gaagattccc gctcccctga gagaccgcat ggaggtcatc caactgccgg
      841 gatacacgcc ggacgaaaaa atcgccatcg gacgcgatta cgccttgccc cgcctgatga
      901 aagacctggg catcaccccg gaaaaagctt ccatccccga caccgacgcg ctcatccgga
      961 ccgtggtgga gaactacaca cgggaggccg gagcgcggca gttggaaagt cgactccgaa
     1021 agatcttggt gaaggcgctg cacgagtgga gccttcggaa agacgcgccg atctcattga
     1081 caccggagaa ggtgctccgc tatctgggac aacctctgga aaccgacgtg atgcgcgacg
     1141 aagacaccgt gggagaggtg atggggctgg cctggacacc cgccggcgga gacatcctct
     1201 tcgttcaggc ctccgtctat gaaaccggcg ggcgcggccc gctttcggtg gacatcaccg
     1261 gaaacctgca aggcgtgatg acggaaagcg cggggctggc cctcaacgtg gcccggcgac
     1321 ggctcgccca aatgggatgg aacgccgaac aactgattgg gaaacggatt ttcatccacg
     1381 tgccccaggg cggcatcccc aaggacggtc cttccgccgg ggtgaccttc gtgacggcca
     1441 ttctctccga actgaccgga cgccccgtcc gccgcgacgt ggccatgacc ggcgaggtgg
     1501 acgccaaggg gaacgtgttg gccatcggcg gcttgaaaga gaaacttttg gcggcgcgga
     1561 aagccggggt caaaaccgtg ttcgtccccg ccgccaacaa ggacgccctg gagggggtct
     1621 tcaaatcggc ccccgaactg cgcggcatcg tcgaacagaa cggccgccac acccttctgg
     1681 tgccgaagac aagttcgacc aaggattggg ccgggctgct gaaacagatc aaggcgaagg
     1741 ggcccggcat cgccgtcacc gagaaaaaaa tcggctcatg ggaaggggtg gaactcgccg
     1801 gaacgcccga ccagatccgg aacctccttg tccattttcc gcagatcgcc cccggccccc
     1861 tgcgttacgt gctggcggaa aaaatcgatg acgtcctcga tcaggccctg atcaccgccg
     1921 agcccccggc ggattccgat cagccccgtt cgccgccggc cgccggcaaa aggtccgagc
     1981 ggggcacggc caccgtcgac gccctcctgg tcctctccgg actgcttctc ctgggatcgc
     2041 tcacactgat tgccgtagga tcgttcgtgc cttggctcgt cggtttcctt ctggacacca
     2101 ccctgtccct cccgcttctc cagtggtctc ttgttgtttc atccccgttc ctccttgtaa
     2161 cggccggaat cgcggtcctg atcatnnnnn nnnnncacgg tctatctggc ccatacgctg
     2221 ttcgcggtca tgaaaaaacg ccgggccctg ggctggttac tggcgttctt tctgggaggc
     2281 caactgatcg tcgacaaact gtgcgactgg ctgacctcct atctcttgcc caacaatttc
     2341 acggggggaa aattccagaa ctcgttatcg ccgccctttc cgatcccctt ccccaaggcc
     2401 cagccggacg tcgatgcgcc gatcgccgac gtgcgggaat tctcggcccg cgccggacgg
     2461 aacgctttct gggagaacga aacggaacgc gccatcacaa aaaacggcgg ttacgcccaa
     2521 atcgtcgacc acgacaacgg ccggccggtc acgcgcattg aaaaactgct tcgctccgtc
     2581 aaggcggagg gggggggcgt ggcccgggtg tggctcttcc gcgacatgcg cgacatggac
     2641 gagctgttcg aaacggtcaa cggcgtccct gtccggatgc ggccggaggt cttcgaggtc
     2701 acccgcgcca tgctcgacgc ccagcggcgc accggcgtga agatcctgcc ctcccttttt
     2761 gatttccata ttctcaacag cgtccggtcg gagacccggg gaacgccgtc cgggctgctc
     2821 acgacgcccg tgggggaaca tccggaattt ttcaccgcga tcgggcggga ccggatgctc
     2881 gaccttttcc gtccctggat gaaagaattc gccgcgcagt tcgacctcat cgatctgttc
     2941 aacgaaccag acaacgtgcg gccggcactc acctccggcg tggcctacaa cccgcacgta
     3001 atgctgttcc tgcaaacctt cgcggctctg gcccacgaac acgggggaaa gatcaccgtt
     3061 ggggtggaca aaatggggcg gctgcaccgc taccagatga tcatccgccc ggatttgggc
     3121 gatgtctaca gcgtccatct ttatggctac aagcccggcc aactgccgcc cgccgggtcg
     3181 ctcggcgtgg gcacggcgcc gatctggatc acggaaatcg gcgcttccac cccggaggaa
     3241 gtggccgctt tcacccggga agccaaggaa aaaggctacc gcggcatcct cttctggccc
     3301 caggacggct tcacccatga aggttacggg cagacgcttc gcgacatgga acagaccgaa
     3361 tccgtcgccg gcccgtcttt aacggaccag cccggggttt ccgcctcgcc gccgttgtcc
     3421 ctggggacga acctcttcgg acggctgctt gccgcgatcc gcctcatggg agaaacccgc
     3481 ccgcccgcca cccgcgcacc gccgacgcgc accgccgaac gcggcagcgc ccggatcacc
     3541 gcgctcctgt tcctctccgg tctgacgctg gccgctttca ttctggccgg gcatctgccg
     3601 gcgtccttcg ccgacctcgg gcacatttcc ctttctcctc tcaccgtcag cgcctcgggc
     3661 atcctcttgg gagggtttct cgtcctcctc gtccggttcc tccgcatgac gaaaccggac
     3721 gccgcgtaca tcacgccgcg ggcgggacgg aattattgga aacataagtt ccttgcgttg
     3781 gcgttcggat tttttctgat tttcggcccc ttctccgccg gcgtcgccgc ccccaccccc
     3841 acgccgtcgt cggacaacgc ggcggtcgcc gcccagttgg agaaacttcc gcgcctctcg
     3901 gcgaaacccc aggtttccct gggagacatc aacgaaaagc tcaccgcgtt gtcgaagata
     3961 tccatgccga ccatccgggg caacctcatg ttcctggacc acgtggtcga cgtcctccag
     4021 gctctgtccc aagacggagc ccttcagaaa aaactcataa agacggaccc cgcccacctc
     4081 ggacgcattt tttacagcat ggaacggatg tccgcggagt gggacaagca gatattcatc
     4141 catgtccatc aggccccggc cgcggaaaag gacgaagccc gccggaaagc ccgccaaatt
     4201 ctcgtcgacg atttttacgc caagcacttc cagcccctcc tttcattctg ggagcaatgg
     4261 catcaagaaa atgacaagta tcaccacctc tcctgcctcc tcggccgctc cctggcctat
     4321 gcggtcaaca tgggcgcccc gtacgcggat tctctgaccg acgcccagtt gcaaaaactc
     4381 cttcaggatc cccaggtggt gatgggtctc tactggaacc tggacacccc gaaacacaac
     4441 gacctcgtca atccggccgc ctctcccacg cacccgttca ctcaggcgct gcgccgccag
     4501 attgggccgg aatggcagga gtttcttctg aaagccattc ccgccaccaa ccaacaggtc
     4561 gccgccatca cgaacgggga aaccgacatc atggattacg gcaacgacat cttcgccctc
     4621 ttggaggagg cggaaaaact gcccaaggac aggccgttgg ccgaactttt cctgaaaaaa
     4681 gcggtcctca acgtgtctct caccgatttc ctccgacggc gaatgacgcc ggaacaatgg
     4741 aacgacccac aaaaacgcgt ccagctgatc aacaacgccc gcggacttct cgccatgatg
     4801 agcgccttcg tcggccccga atacgtcgcc aaaaccggcg aggtcagcgc cttcgccagc
     4861 taccccgcca tcaccttcgg cagttcgcac cgctacgcca tcgacctttc ccgcatcggc
     4921 catgaggccg tccatcagct cgaccgggcc atgtggttca tcctcaacga cccctccatg
     4981 aacgactact gggcctgggg gttcggagac cacaccctca acatcccgtc ccggttcgac
     5041 cgctaccttt tgaagaactt ctacccaccg ccgctcaccc ccctggtgga cgtccctctt
     5101 ctggcctcgg agatggaaac gagccacgtg ctggagttcg tggcccgcaa aaacaataag
     5161 accatcgccg acgttttggc ggacgtgaga aaacgctccc cggaagccgt cgcccaggac
     5221 ctcctccgct gggtgcgcga cgagaacatc gccgaggtgg aatacgtcgt caaccaacag
     5281 gtcggcattt ccgccgccta cggacgccgg gcgaactccg atttcacgga tccctcatcg
     5341 gtctttgaga taccgaaggt cgtgaaacgg accaccacgc tgaaatccct ttatcaacac
     5401 cgcctgcaaa acatcctggc gggcatgcgg ctcgcccagg aatcctcctc gccgcaggaa
     5461 gcctacctcg ccatcatgcg caagatctac gaacagccca agaacatgac ttacgcggaa
     5521 ttcgtgaacg ccatcggcac cgccaacacc caagacgtgc gcgacctcct gtcaaaacac
     5581 tttaaagacc ggaactacac ctacgaaaaa ctcatcaagg atgtcgcttc ccgcacccat
     5641 cccctcggca aaaaagtcgt ggagatcggg atgggacgcg acaccgtcgg ggtcgagggg
     5701 cagatcgacc ggtccggcgc gggatacatg ctggcggggc tcatctatgc catcgccggc
     5761 aacgatcacg ccaaggccga ggaacttcag cgtaacctga gccactttga aaccctcctg
     5821 cacgccttcg acggtctcga actgcccaaa gtgagcgcca acaacgtttc accggacccg
     5881 gaacaaccgt tctatcggac ggcaccgcct tatgtcgccg gacaggcggc tcacctgacg
     5941 gcgccggccc atggggtctc cgcctcttcc tataaaggaa ggagtcaaac ggcgcaggtc
     6001 cgacggcttt atacccctca aaaagtcccg tcacgtcacg cgcaaaccaa agtgccctct
     6061 tcctggaacg ccgccctggt ggtgggggga ctcgtcctgg ctttcggcct cttcaagttc
     6121 ggctggccgg cgctcttctc cctgctgttg atgcccggcc ggctctggta tgtcctgatg
     6181 gcccggccgg gttggcccac gctcaccccg tccatgcagg ccgagttcaa gaacaaactg
     6241 caggcgaccc tcggctcccg attcggacgc gtggagatct tcgatttccc cggcgaaacc
     6301 cgaagcaccc tgtcgcttct gaaatctccc tacgcgcaac tgacgccgtt ggcctacgtg
     6361 acgccttccg cggcggaagg cgaaggggac atcctttatc tccaccgcag cgtggcgttc
     6421 gctttgtcgg actacaacga caaaacctcc acgccgaacg tcctcctccg ctccctgacg
     6481 cgcgccgtgg cggaaaccgt tctcctccac gaactctccc agcacggccg acgggaaagc
     6541 gcggggatcc tggcgcagtt cggatggggc cggaaattaa ggccgttcgt ctccccgaaa
     6601 ctcttcctgg cgaggctcat cgtcaatctg gccgacgtgc cgcaactcgt ttgggcccgg
     6661 gtgggggccc tgcgccccat caaaaccccg g
//
LOCUS       DAOTFD010000139         6593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8331_length_6593_cov_4.284185, whole genome shotgun sequence.
ACCESSION   DAOTFD010000139 DAOTFD010000000
VERSION     DAOTFD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_6593_cov_4.284185"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..704
                     /locus_tag="P5079_08130"
     CDS             153..704
                     /locus_tag="P5079_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29985.1"
                     /translation="MLFASDAGGETFHRGRWRTVTRRATGCGTRQGCSSRNGTFLIGG
                     EESGGLSINGHVPEKDGILACLLAAEIVAVNKKPLRKILDALQKEVGSFCTKRLNFYL
                     PHDKMVALREKLRENPPTRFGGLTVRRIVETDGHKFILTDGCWIGVRLSGTEPVVRVY
                     LEAHDQEKLKVLEKAGRVLAGVP"
     gene            788..2161
                     /gene="eno"
                     /locus_tag="P5079_08135"
     CDS             788..2161
                     /gene="eno"
                     /locus_tag="P5079_08135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY29986.1"
                     /translation="MILKKSDRRSNSRSSSSRPPARSSAKISNIRAREILDSRGNPTL
                     EVDVLLMDGVLGRAAVPSGASTGIHEALELRDGDPKRYMGKGVLKAVGHVTRELAPAL
                     QGKDPCDQRGLDELMLKLDGTETKSKFGANAILGVSLAVARAAAKSRRVPLHEHIRNA
                     FGVRSDEFLFPVPLMNILNGGAHADNNVDLQEFMVAPVCGGSFREALRAGAEVFHALK
                     GVLKKSGYNTGVGDEGGFAPNLKSHEEALLVILRAIEQAGYQPGKDVCLALDCAANEY
                     FENGRYRLEGESVVKDRSAAEMVEFYGQWVQKYPIVSIEDGLAEDDWEGWKQLTDKLG
                     SRVQLVGDDLFVTNVKRLQDGIRKGVANAILVKVNQIGSLSETVDAVMKAKEAGYATV
                     ISHRSGETEDSFIADLALALNAGQIKTGSASRSERIAKYNQLLRLEESLGKKAKFPGL
                     SAFPRAR"
     gene            2232..2570
                     /locus_tag="P5079_08140"
     CDS             2232..2570
                     /locus_tag="P5079_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRY29987.1"
                     /translation="MSPFFRRHFVWIILGVVLLGWLLSSGGIRNYFLRLREYRRLDAR
                     RRETKQRVAEKQVRLNKAMKDESFLELEARRHLGLVRPDEIEYRFVASTGASPSDQQM
                     IKTFPAPNNP"
     gene            2642..3079
                     /locus_tag="P5079_08145"
     CDS             2642..3079
                     /locus_tag="P5079_08145"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRY29988.1"
                     /translation="MLVEPDIPWNTGNVGRTCLGWGAELHLVGKLGFSLEDRYLKRAG
                     LDYWPSVKLFVHPDAGEFFRDFPPGNAFFLSARAKKPFWRARIRPGAALVFGSETQGL
                     PTSLRKRFRSRFYRIPFTGPIRSFNLSTAAGMVLGEAVRQNSR"
     gene            complement(3292..3858)
                     /locus_tag="P5079_08150"
     CDS             complement(3292..3858)
                     /locus_tag="P5079_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRY29989.1"
                     /translation="MKKIAILFGIIAVFLLMPGCVGVLYYNRLVSLHEAVGAGWAQVE
                     NVLQRRYDLIPNLVNTVKGYAKHEKELFEEVTRLRSQWGEAKTPAEKVRASGGLEAAL
                     SRLLLVAERYPDLKANQNFLALQDELAGTENRIAVERMRYNEAVRNYNILTRRFPSNV
                     VAGLTGFETSDNYFKAETEAKAAPPVQF"
     gene            complement(3887..4600)
                     /locus_tag="P5079_08155"
     CDS             complement(3887..4600)
                     /locus_tag="P5079_08155"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY29990.1"
                     /translation="MMTPENLAQELKNIYGDNLVSVVLFGSAATGEHVDKKSDYNVLV
                     LLTRADLAALRLFAAPARRWVKDGNPLPLFFDERHLRRSLDAFPIEFADIRDGRRVLH
                     GADVFVNIPINEELLRLELENELKGKLLQLKQRYLLVAGDAQGLKSLMTESLSTFLNL
                     FRNALRLFEPVPPVQKMAALGALAKHLPLDAAVFKEIDDLRRKGKGTEAGLENIFERY
                     VTAVEAVVEAVDKHIQSKK"
     gene            complement(4666..5388)
                     /locus_tag="P5079_08160"
     CDS             complement(4666..5388)
                     /locus_tag="P5079_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRY29991.1"
                     /translation="MPTSFRRFLFLSAPGFLLLSTALAGTWPAPRGMLNDFAGIFPPE
                     KSGELEDRLTELKEKTGAEVAVVTVSEIPGGDVDGAATDLFAAWGVGQEGKNNGVLIL
                     CSIEQRRVRIEVGYGLEGLLPDALCGRIMDQHMIPWFRRGDIPQGLIDGAAAVAEVIA
                     KDAGATLRHAPAAAPAEEEIPRWVWFVILLALLIASGFLRRRYGAGPYGGGFYGGGFS
                     GGGGGGGFGGGFSGGGGAGRSW"
     gene            5494..5655
                     /locus_tag="P5079_08165"
     CDS             5494..5655
                     /locus_tag="P5079_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29992.1"
                     /translation="MADVLCPKCNEKGNVVVKKSRTDKGDEMKYFECKKCRSLWTNSA
                     DIPNLPVEG"
     gene            5698..>6593
                     /locus_tag="P5079_08170"
     CDS             5698..>6593
                     /locus_tag="P5079_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY29993.1"
                     /translation="MSRKSSSGAAEVGMPFLRLSPRWCFLLYACLAGLCLVLHNRMVD
                     SAATDARRTLHAQVMANTAAAPLQYRVVTFYAAEGVQRLADALGVDDFFHGSYLLLRG
                     LATFFSAVLLHRFLAVYFPLPVALLGTVLAFAWLPLTYINYYMQETDPLNMIFFLLGY
                     ELIRRRKDAALAGLLPLAMLNRETPVLLPLVWLLYRWDELPAGRLLLKFFGLCAAAFG
                     PYFGLRFLFGHHGAYAEFNDLALNLKSVNSYLYFFLLFGALAVPAIRGFSGKPKFLRR
                     SALFLPFFLVFHFVIPIFQETRL"
BASE COUNT         1155 a         2034 c         2022 g         1382 t
ORIGIN      
        1 cgggtgatcc tgcgcccgcg cttgccgcgc ttcgcatcgt ggctcgattc gctccgtttc
       61 gactcggctg ttaccgcttt cccgggcgca tgttgctcga ggaacgagcg gcagatggat
      121 gcggacctcg tgtagcctcg cgggggtttg atgtgctttt cgcgagcgat gctggcggcg
      181 aaacgtttca tcgcgggcgt tggcgcacag tcactcgccg cgccaccggc tgcggcacca
      241 ggcaaggatg ctcctcccgc aacggcacct tcctgatcgg cggagaggaa tccggcgggc
      301 tgtccatcaa cgggcacgtg ccggaaaagg acggcattct ggcgtgtctt ttggcggcgg
      361 agatcgtggc ggtgaacaag aagccgctgc ggaagatttt ggacgcgctg cagaaggaag
      421 tggggagttt ctgtaccaag cggctcaatt tttacctccc gcacgataag atggtggccc
      481 tgcgggaaaa actccgcgaa aatcctccca cgcggttcgg cgggctcacg gtccgccgca
      541 tcgtcgagac cgacggccat aagttcatcc tgaccgacgg ctgctggatc ggcgtccgtc
      601 tgtccggcac ggaacccgtg gtgcgggtgt atctggaagc ccacgaccag gaaaaactga
      661 aagttctgga aaaggccggc cgcgtactgg ccggcgtgcc ttagcgccgg cccggctcag
      721 agccggaccc ggcgcccctc gtcgttcgtc cgcacgattt taaatccata ttaaaggagt
      781 cgtttccatg atattgaaga aaagcgatcg ccgttccaat tcccgttcga gctccagccg
      841 gccgccggcc cgcagttccg ccaagatatc caacatccgc gcccgggaaa tcctcgattc
      901 ccgcggcaac cccacgttgg aggtggatgt tctcctgatg gacggcgtgc tcggccgcgc
      961 ggccgttccc tccggcgctt ccaccggcat tcacgaggcc ctggagttga gggatggaga
     1021 ccccaaacgc tacatgggga aaggcgtttt gaaggcggtg ggccacgtta cgcgggaact
     1081 ggcgccggcc ttgcagggga aagacccgtg cgatcagcgg ggtttggacg agctcatgct
     1141 gaaattggac ggcacggaga cgaaatccaa gttcggcgcc aacgccatcc tgggtgtttc
     1201 gttggcggtg gcgcgggcgg cggcgaaatc gcggcgcgtg ccgctccatg agcacatccg
     1261 taacgctttc ggcgtccgct ccgacgagtt cctctttccc gtgccgctga tgaacattct
     1321 caacggcggc gcccatgccg acaacaacgt ggacctgcag gaattcatgg tcgcgccggt
     1381 ctgtggcggg tcttttcgtg aggccttgcg cgccggcgcc gaggtgttcc atgccttgaa
     1441 gggcgtcttg aagaaatccg gctacaacac cggggtcggc gacgaggggg gattcgctcc
     1501 caacttgaag tcccacgagg aggccctgct ggtcatcctt cgcgccatcg agcaggccgg
     1561 ttatcagccc gggaaggacg tctgcctggc cctggactgc gccgcgaacg aatatttcga
     1621 gaacggtcgt taccgcctgg aaggcgagtc cgtcgtgaaa gaccgctccg ccgccgaaat
     1681 ggtggaattc tacgggcagt gggttcagaa atacccgatc gtttccatcg aggacgggct
     1741 cgccgaggac gattgggaag gttggaaaca gctgaccgac aagctgggat cccgggtcca
     1801 gctggtgggg gacgacctct tcgtcacaaa cgtgaaacgc ctgcaagacg gcatccggaa
     1861 gggcgtggcc aacgccatcc tggtgaaagt gaaccagatc ggttccttga gtgagaccgt
     1921 ggacgcggtc atgaaagcca aggaggcggg gtacgccacc gtcatctccc atcgttccgg
     1981 cgagaccgag gattctttca tcgccgactt ggccctggcg ttgaacgcgg gccagatcaa
     2041 gaccggttcc gcctcgcgtt ccgagcggat cgccaaatac aaccagctcc ttcgcttgga
     2101 ggaaagcctg ggcaaaaagg ccaaattccc cggcctgtcg gcttttccgc gcgcccgcta
     2161 gtttccgtta cggtccggcg cccggtggat gtcggtgacg ccggccctct ttccggccgg
     2221 agaagatagg aatgagtccg tttttccgtc gtcatttcgt ctggatcata ctgggggttg
     2281 tccttctcgg gtggcttttg tcgagcggcg ggatccgcaa ttatttcctg cgcctgcggg
     2341 aatatcgccg gctggacgcc cgccggcgtg aaaccaaaca gcgcgttgcc gagaaacagg
     2401 tgcgcctgaa caaggccatg aaagacgaat ccttcctgga attggaggcc cgccggcacc
     2461 tggggctcgt gcggccggac gagatcgaat accggttcgt cgccagcacc ggcgcctctc
     2521 cgtccgacca gcagatgata aagacgtttc ccgccccgaa taatccctga gccccgtcat
     2581 gtccgtccgt cgaaaaaaaa cgaaaaaaaa cccttgcggg gcggcggctc cgtttcgggt
     2641 agtgttggtg gagcccgata tcccctggaa cacggggaat gtggggcgaa cctgtctggg
     2701 gtggggtgcg gaactgcatt tggtggggaa actcggcttt tccttggaag accgctatct
     2761 gaaacgcgcc ggcttggatt attggccgtc ggtaaaactc ttcgtccatc ccgacgcggg
     2821 ggaatttttc cgcgattttc cgccgggaaa cgcttttttt ctttccgccc gggcgaaaaa
     2881 gccgttttgg cgcgcccgta tccgtcccgg cgccgccctg gtgttcggtt ccgagaccca
     2941 ggggctgccg acatccctcc ggaaacggtt ccggtcccgg ttctatcgca tcccgttcac
     3001 cgggcccatc cgttccttta atctttctac cgcggccggg atggtcctcg gtgaggccgt
     3061 gcggcagaac tcgcgctgac tcccccgctg tccaaatcgg aaacaaggcc attttgtcgt
     3121 tcaggcggat ggaatttctt gtagcgccgt tcccgcctcc gtgtttctgg agacggtccc
     3181 cgctcgttga aggggaaagc gggaatccgg atccgaagca atccctgtcg acggctaacc
     3241 gggctcccgg cgagaagcat gccgggacga cacatccgtg cagagtttag gttaaaactg
     3301 caccgggggg gccgctttgg cctccgtctc cgctttgaaa taattgtccg acgtttcgaa
     3361 gcccgtcagt ccggccacca cgttcgacgg gaagcggcgt gtgaggatgt tgtaattccg
     3421 caccgcttcg ttgtatcgca tccgttccac cgcgatgcgg ttttcggtcc ccgccagttc
     3481 gtcctgcagg gcgaggaagt tctggttcgc tttcaggtcc ggatagcgtt ccgccaccag
     3541 cagcaggcgg gagagggcgg cttccagtcc gcccgaggcc cgcacctttt cggcgggggt
     3601 cttggcttct ccccactggc ttcggagccg cgtcacttcc tcgaagagct ctttctcatg
     3661 tttggcgtag cccttgaccg tgttcaccag gttggggatc aggtcgtagc gccgttgcag
     3721 gacgttctcc acctgggccc accccgcccc cacggcttcg tgcagggaca ccaggcggtt
     3781 gtaatacagc acgcccacgc agccgggcat gagaaggaaa acggcgatga ttccgaacag
     3841 gattgcgatt tttttcatga agaggtcctc cttgagggat gaaaattcat ttttttgatt
     3901 ggatgtgctt gtccaccgct tcgacgacgg cctccacggc cgtgacatac cgctcgaaaa
     3961 tgttttccag cccggcttcg gttccttttc ccttccgccg gaggtcgtcg atttccttga
     4021 agacggcggc gtccaagggc aggtgtttcg ccagcgcgcc cagggccgcc attttctgaa
     4081 ccggcggcac cggttcgaac agccgcagcg cgttccggaa gaggttcagg aacgtggaga
     4141 gggattccgt catcaaggat ttcagccctt gggcgtcccc cgcgaccagg agataccgtt
     4201 gcttcagttg gagcagtttc ccttttagtt cgttctccag ttccagacga aggagttcct
     4261 cgttgatggg gatattgacg aaaacgtcgg cgccgtgcag gactcggcgg ccgtctcgga
     4321 tgtcggcgaa ctcgatcgga aaggcgtcca aggagcggcg gagatgccgc tcgtcgaaga
     4381 agagcggcaa gggattgccg tctttcaccc aacggcgggc tggggcggca aagagacgca
     4441 gggccgccag gtcggcgcgc gtgagaagga cgaggacgtt gtagtcggat tttttatcga
     4501 cgtgttctcc cgtcgccgcc gagccgaaca gaaccacgct gaccaggttg tccccgtaga
     4561 tgtttttcag ttcttgggcc agattttccg gagtcatcat gagatcttct ctttcagggc
     4621 cgtcgtccga agacgagagc gcgcttcctt ataaattatc aaattttacc agcttcgtcc
     4681 ggctccgccg ccgccggaga acccaccgcc gaacccgccg ccgccccctc cgccggagaa
     4741 ccctccgccg taaaacccgc cgccgtaagg tcccgccccg tatctccttc tgaggaatcc
     4801 cgaagcgatg agcaaggcga ggaggatgac gaaccaaacc cagcggggga tttcttcttc
     4861 cgccggtgcg gcggccgggg cgtggcggag cgtcgctccc gcgtccttgg cgatgacctc
     4921 cgcgaccgcc gccgcgccgt cgatcagtcc ctgggggatg tctccccggc ggaaccaggg
     4981 gatcatgtgc tggtccatga tccggccgca gagggcgtcc gggaggaggc cttccagccc
     5041 gtaacccact tcgatccgga cccggcgctg ttcgatggaa caaaggatca agaccccgtt
     5101 gtttttccct tcctgtccga ctccccacgc cgcgaaaagg tccgtcgccg ccccgtccac
     5161 gtcgccgccg ggaatttcgg acaccgtgac cacggccact tcggcgcctg ttttttcctt
     5221 caattcggtc agccggtctt ccaactcgcc ggatttttcc ggcggaaaga tcccggcgaa
     5281 atcgttcagc atccctcgcg gggcgggcca ggtcccggcc agggccgtcg acaggagtag
     5341 gaaccccggg gcggagagaa aaaggaatcg acggaacgaa gtcggcatga acctattgta
     5401 gcgaaacggg ggccgttccg acaggcttcc cccggcctct cgaatgtttt agaatacgct
     5461 ccggttgaaa aaaacggctc cgggaggtct cctatggctg acgtgttgtg tccgaaatgc
     5521 aatgagaagg ggaatgtggt ggtgaagaag tcccgcacgg acaaggggga cgaaatgaaa
     5581 tattttgaat gcaagaaatg ccgaagtctc tggaccaatt ccgccgacat tcccaacctg
     5641 cccgtcgaag ggtgacgccc gccttgtttc aggtttttcg gcttccgaaa agtccgtatg
     5701 tcgcgtaagt cctcttccgg cgccgccgaa gtcggcatgc cgttcctccg tctttccccg
     5761 cgctggtgtt tcctcctcta cgcctgcctc gccgggctgt gtctggtcct gcacaaccgg
     5821 atggtggact cggccgccac cgacgcccgc cgcacgctcc acgcgcaggt gatggcgaac
     5881 accgccgcgg cgccgctgca ataccgcgtg gtcacgttct acgccgccga gggggtccag
     5941 cgcctggcgg acgccctcgg ggtggacgat ttcttccacg gttcctatct gctcctgcgg
     6001 ggtctggcca cttttttttc cgccgttctc ctgcaccgct tcctggcggt ctatttccct
     6061 ctgccggtcg cgttgctggg gaccgtcctc gccttcgcct ggcttccgct gacctacatc
     6121 aactactaca tgcaggaaac ggaccccctg aacatgatct ttttcctttt gggttacgaa
     6181 cttatccgtc gacggaaaga cgcggcgctg gcggggcttc tgcccctggc catgctgaac
     6241 cgcgagacgc ccgttttgtt gccgctggtg tggcttctct atcgatggga cgagctgccg
     6301 gcggggaggc tcctcctgaa attcttcgga ctgtgcgccg ccgccttcgg tccctatttc
     6361 ggactgcgct tccttttcgg ccatcacggg gcctacgcgg aattcaacga cctcgccctc
     6421 aatctgaaga gcgtgaactc gtatctttat tttttcctgt tgttcggggc gctcgcggtg
     6481 ccggcgattc ggggattttc cggaaaaccg aaatttcttc gacggtcggc gttgttcctg
     6541 ccgttcttcc tcgtctttca tttcgttatc cccattttcc aggagacgcg tct
//
LOCUS       DAOTFD010000140         6575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8357_length_6575_cov_4.372853, whole genome shotgun sequence.
ACCESSION   DAOTFD010000140 DAOTFD010000000
VERSION     DAOTFD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8357_length_6575_cov_4.372853"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..262
                     /locus_tag="P5079_08175"
     CDS             26..262
                     /locus_tag="P5079_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRY29994.1"
                     /translation="MDEPVIVEKSPVVLELDAGTYHWCACGRSKKQPFCDGSHKVTAF
                     HPVAFTLTEKRKVVLCRCKHTKNPPFCDGTHKTL"
     gene            complement(447..2756)
                     /locus_tag="P5079_08180"
     CDS             complement(447..2756)
                     /locus_tag="P5079_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY29995.1"
                     /translation="MPDTPTEKHPSPRTGPRSWRLLLAVLLGLLHAGSARLLADEPAG
                     PWTIGGRVLEKKYGRPLAGARLFVEENKSQRAAADAQGAFCLEVPGPGRYHLAVLHED
                     LQNAPPDVVVVSPEQPRVTVVLQREIRPPALPDIVVTAERHKDKTSKRVITGEELSNV
                     AGSAGDPIKALQALPGITSGNDSSGDPAIRGSAPHDNLYYVDGMPVGYLFHMGDFYSI
                     FNGDLVEDFSIFPSAFGPEYRSANGAVIDVALREPRRDKFVTTLNVSTLESDVLFEGP
                     TAKDQAFYLGARRSYLDLLLPKTGSLDSGIDYVQFPKFYDYQGKYVWRLSSRNTLSLM
                     GSGAEDESSLKIREDSDIAAHDPALAGDFSSLVRYHSQGISLTSLITPALTNKLIVGG
                     LLTGFDLQAPIGYVRLNFHDLFVNEQLTITPDDAHEISLGGEFFNEAGSISADIKYEA
                     SSDFDPGRTDFTSASRKTYRKNLYSNAYAVHAKDRWQLFPVLMLGIGGRASYDDYLGG
                     LYLEPWLGSEYNLTEKTLLTAAWGRYHQTPEGEEMVDVFGNEDLTHIRSEHTVAGIGQ
                     TLDHGWGWKLDVYYKELRELVVPDDTEIFVNRGRGTAKGAEFLLKKKQTTRWAGWVSF
                     SQAESTRRNDLTGQEINFKYDQPSLANLVGQYDLTEKWTLSGHWRYHSGSPYTPIVGR
                     TYDGVRYHPVYAEVNSARFPDYHRLDIRLAYHSRKDKRLDSWTVYFDIINAYNQRNIG
                     GYSYNADYSSKKAVHQLPFMPAFGFTMRF"
     gene            complement(2771..4435)
                     /locus_tag="P5079_08185"
     CDS             complement(2771..4435)
                     /locus_tag="P5079_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY29996.1"
                     /translation="MEPPRPPLSLLERLPRHGPLLTGLVTLAVFWPCLQNGFVHWDDG
                     ANFLDNPAYRGFSWPQLRWMFTSFHLGHYIPLTWMSFSLDHTLWGMNPAGYHLTNVLL
                     HAANAVLFYFLAERLFRLSPGRPPAETAGAVAAASLAAALFFSLHPLRVESVAWVTGR
                     KDLLAGFFILLSSLLYLRHASRGTAGRYIACLGLFLFALLSKGVSLVLPFVWLALDFF
                     PLRRLTDGPSLRRCLKEKIPFLALSTVFAAVALAGARFSGAAMPLTEYDPQQRLGEFF
                     FSSLFYLHKTLLPFSLSPLYPLADDLRLNVSSALLHGLVFALLTVLAFLYRKKAPAGI
                     ALWTVYLAFLAPTSGLIQSGPQTVADRYSYLSCFGWALLAGGAVRARFPQGRGRRILA
                     LLPALGLFSLLSVLSHKQIGVWKDSQTLWSHVLTVTPPSSIAHLGYGMALEETGEVDR
                     AEEHYRKALALRPTFDLALRRLAGLRLRKGEWRQAAGLYQLSLAFQPNDAEALNNLGY
                     SLLRLHMPVEAAACLSRALALMPDSEKIKHNLHLAHAAGRRPAEKR"
     gene            complement(4453..>6575)
                     /locus_tag="P5079_08190"
     CDS             complement(4453..>6575)
                     /locus_tag="P5079_08190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY29997.1"
                     /translation="PPDGFPFTGAFVMNCPKCKKNMEEVYSPQGVLIDACPACAGVWF
                     DRNEISFFVKEPKRLHQEITAPAGRTSTPHPCPRCRDPFLQEQGLFSNEIKVDVCPKC
                     SGVFLDADELEKINAVLGRAVKSGKVARALPVGLQDLADRIHRPLAAAATLPLQTLPN
                     LGLRSLGVFGVLSGLVFLAFFFLAEVARWQPHTAAAMALGFLFLQYLLSPLLMDWTLR
                     WTMSLRWVDFTELPPALQQFVSRVCAEENVKIPRFGIINDGTPNAFTYGHTPNNARIV
                     VTRGLMEILTEGELNAVVAHELGHAVHWDIAVMTLAAAVPILLYYLFRVLWNLAAKAS
                     SSRGKDSAKGAAPLYVAALAAYLIYLVSEFIVLYLSRVREFWADRFAGEKTRNPNELA
                     MSLVKIAYGLAGGEEKKGKQPERTGRLDTVQSLSIFERKGALALAAMSLSQGNKLSLS
                     SVVAAMQWDLWNPWAGYYEIQSTHPLPAKRIAALSHQSMTYGKMPLVEFNREKPESYW
                     DEFLVDVLMTFLPALSVLAGLGALWAAGLLFETDKLRLFMQLLFLFGFGSFLKTLFSY
                     RFDFFPDLRVADLLKNVKVSRVRGVPCRLHGKIIGRGVPGLIWSEDLVLQDDSGYIFL
                     DYRQPLGLWELLFGLFRAGGLVGQTVTADGWYRRGPVPYVELHRLILNNGDTKTCYVY
                     AVKWTVSVLLMVLGAALSVAAFLG"
BASE COUNT         1263 a         1724 c         2423 g         1165 t
ORIGIN      
        1 agcttttttc taaggaggac atgtcatgga tgagccggtg attgttgaga agagccccgt
       61 ggttcttgaa ctggacgcgg ggacgtatca ttggtgcgct tgcggtcgtt ccaagaagca
      121 gcctttttgc gacgggtcgc acaaagtgac ggcgtttcat cccgtggctt ttacgctcac
      181 cgaaaagaga aaagtggttc tgtgccgatg caaacacacc aagaacccgc ctttttgcga
      241 cggcacacat aagacgctgt aagagaaagt ccgctgtggc ggagaggggt tgttgagctg
      301 ggggtcttct tgtttcacgg gtgttttccg gaaataggcc ggggatcccc gttcttcgat
      361 tcaaaacaga ccgaaacatc cggattcaat ccaaacaaga aaaagttacg gcccggcgtt
      421 acatgaaaac accggcgggg cgtgttttag aaccgcatgg tgaagccgaa agcgggcatg
      481 aacggcagtt gatgcaccgc tttcttggag gagtagtcgg cgttgtagga atagccgccg
      541 atgttccgct ggttgtaggc gttgatgatg tcgaaataga cggtccagct gtccaggcgc
      601 ttgtctttcc ggctgtggta ggcgagccgg atgtccaggc gatggtaatc cggaaagcgg
      661 gcggagttga cctccgcgta gaccggatgg tagcggacgc cgtcgtaggt cctgccgacg
      721 atgggtgtgt agggcgagcc gctgtggtag cgccaatgcc cgctcagggt ccatttttcc
      781 gtgaggtcgt attgaccgac gaggttggcc agggagggct ggtcgtattt gaaattgatc
      841 tcctggccgg tcaggtcgtt gcggcgcgtg gattcggcct gggaaaaaga gacccagccc
      901 gcccatcgcg tggtctgttt cttcttcaga aggaactccg cccctttggc cgtcccccgg
      961 ccccggttga cgaaaatctc cgtgtcgtcc ggcaccacca actcccgtaa ttccttgtaa
     1021 tagacgtcca gtttccagcc ccagccgtgg tcgagcgtct gtccgatgcc ggcgacggtg
     1081 tgttccgagc ggatgtgggt gaggtcttcg ttgccgaaga cgtccaccat ctcttcgccc
     1141 tcgggcgttt ggtgataccg cccccacgct gcggtgagga gcgttttttc cgtgaggttg
     1201 tactcgctcc ccagccaggg ttccaagtag agccctccca gataatcgtc gtagctggcg
     1261 cgccctccga tgccgagcat aaggacggga aacaactgcc accggtcctt ggcgtggacg
     1321 gcgtaggcgt tgctgtagag attctttctg taggttttcc gggaggcgga ggtgaaatcc
     1381 gtgcggccgg ggtcgaagtc ggaggaggct tcgtatttga tgtcggcgga aatggaaccc
     1441 gcttcgttga agaattcgcc gcccaggctg atctcgtgcg cgtcgtcggg ggtgatggtg
     1501 agttgttcgt ttacgaagag gtcgtggaag ttgagacgga cgtagccgat gggggcctgg
     1561 aggtcgaatc ccgtgaggag tccgccgacg atgagtttgt tggtgagggc gggggtgatg
     1621 agggaggtga gggagatccc ctgggaatgg taccggacga ggctggaaaa atctccggcc
     1681 agggcggggt cgtgggcggc gatgtcggaa tcctcccgga tcttgaggga actctcgtcc
     1741 tcggcgccgc tgcccatcag cgagagggtg ttccgggagg agagccgcca gacgtatttg
     1801 ccctggtagt cgtagaactt ggggaattgc acgtaatcga tgccggaatc caggctgcct
     1861 gtttttggaa ggagcaggtc caggtagctg cgccgggcgc cgagatagaa cgcctggtcc
     1921 ttggccgtgg gaccctcgaa gaggacgtcc gattccaggg tgctgacgtt gagggtggtg
     1981 acgaacttgt cccggcgggg ttcccgaagg gccacgtcga tgaccgcgcc gttggcgctg
     2041 cggtattccg gcccgaaggc ggaggggaag atgctgaaat cctcgacgag gtcgccgttg
     2101 aagatgctgt agaaatcgcc catgtggaag agatagccca ccggcatgcc gtccacgtaa
     2161 tacaggttgt cgtggggggc ggaaccccgg atggccgggt cgccgctgga gtcgttgccg
     2221 ctggtgatgc cgggcagggc ttgcagggcc ttgatggggt ccccggcgct gccggcgacg
     2281 ttggagagtt cttcgccggt gatgacgcgc ttgctggtct tgtccttgtg ccgttccgcg
     2341 gtgaccacga tgtccggcag ggccggcggc cggatctccc gctgcagcac gaccgtgacc
     2401 cggggctgtt ccggcgagac aacgacgacg tcgggcgggg cgttttgcag gtcctcgtgg
     2461 aggacggcca ggtggtatcg gcccggtccc ggcacctcga ggcagaaggc cccctgggcg
     2521 tccgccgcgg cgcgttggga tttgttctct tccacgaaga ggcgggcgcc ggcgagcggc
     2581 cggccgtatt ttttttcgag gacgcggccg ccgatcgtcc agggaccggc cggttcgtcg
     2641 gccaggagcc gggcggaacc ggcgtgcaga aggccgagga gaacggcaag aagaagacgc
     2701 cacgagcgag ggcccgtacg aggggagggg tgtttctcgg tcggggtgtc cggcatgagg
     2761 agacgagagg ttaacgtttt tccgccgggc ggcgtccggc ggcgtgcgcg agatgcaggt
     2821 tgtgtttgat tttttccgaa tccggcatca gcgccagcgc ccgggaaaga caggccgcgg
     2881 cttcgacggg catgtgaagg cggaggaggc tgtagcccag gttgttgagc gcttccgcgt
     2941 cgttcggctg aaaagcgagg gagagctgat acaggccggc ggcctggcgc cattcccctt
     3001 tgcggagtcg gagcccggcg agccgccgga gggccaggtc gaaggtgggc cggagggcca
     3061 gggcttttcg gtagtgttcc tccgcgcggt ccacttcgcc ggtttcttcc agggccatgc
     3121 cgtatcccag atgggcgatg gaggaggggg gtgtgacggt cagcacatgg gaccaaagtg
     3181 tctgcgaatc tttccagacg ccgatctgtt tatggctgag aaccgagaga agggaaaaaa
     3241 gaccgagagc gggaaggagg gccaagattc gtcggccgcg gccctggggg aaccgggccc
     3301 gcacggctcc gccggccagg agggcccagc cgaaacacga gaggtagctg taccggtcgg
     3361 cgacggtctg cgggccgctc tggatcaggc ccgaggtggg cgccagaaaa gcgaggtaga
     3421 cggtccagag ggcgatcccc gccggcgctt ttttccgata gaggaacgca aggacggtca
     3481 gaagtgcgaa gacgaggccg tgaagaagcg cggacgaaac gttgaggcgg aggtcgtccg
     3541 ccaggggata gaggggcgac agggagaagg ggaggagcgt tttgtgaaga tagaagaggg
     3601 aactgaagaa gaactcgccc agccgttgtt gcgggtcgta ttcggtcagc ggcatcgccg
     3661 ccccggaaaa gcgcgccccg gccagcgcca cggccgcgaa caccgtcgac aacgccagaa
     3721 aggggatctt ctctttgagg caacgccgga gagacggacc gtccgtgagg cggcggagcg
     3781 gaaagaaatc cagcgccagc cacacgaagg gcaggaccaa actgacgccc tttgaaagga
     3841 gggcgaaaag aaacagaccc aggcaggcga tgtatcgtcc ggcggttcct cgggaggcgt
     3901 gtcggaggta aagcagcgag gagagcagga tgaaaaagcc ggccagaaga tctttccggc
     3961 cggtgaccca ggccaccgat tccactcgga gaggatgcag ggagaaaaag agggcggccg
     4021 ccaacgaggc cgcggcgacc gctccggcgg tctcggccgg ggggcggccc ggcgaaaggc
     4081 ggaacagccg ctcggcgaga aagtaaaaca ggacggcgtt ggccgcgtgc agcaggacgt
     4141 tggtgagatg atagccggcg gggttcatcc cccagagggt gtggtcgagt gaaaaactca
     4201 tccaggtgag tgggatgtag tggccgaggt ggaacgaggt gaacatccaa cggagttgcg
     4261 gccaggaaaa tccgcggtag gcggggttgt ccagaaaatt ggcgccgtcg tcccagtgaa
     4321 cgaacccgtt ttggaggcac ggccagaaga ccgcgagggt gacgaggccg gtgaggagcg
     4381 gcccatggcg ggggaggcgt tccaagaggg agaggggcgg tctcggcggt tccatggacg
     4441 tcttggagga ggtcagccga ggaaggcggc gacgctgagg gcggcgccga ggaccatcag
     4501 gaggacggag accgtccatt tgacggcgta cacgtagcac gtcttcgtgt cgccgttgtt
     4561 caggatgagc cggtgcagtt ccacgtaggg caccggcccg cggcggtacc agccgtccgc
     4621 ggtgaccgtc tggccgacga gtcccccggc gcggaaaagg ccgaacagaa gttcccatag
     4681 tcccagcggc tggcggtagt ccaggaaaat gtagccggag tcgtcctgga ggaccaggtc
     4741 ttccgaccag atgagcccgg gcacgccgcg gccgatgatc ttcccatgca ggcggcaggg
     4801 cactccgcgc acccgggata ccttgacgtt tttcagaaga tcggcgacgc ggaggtccgg
     4861 gaagaaatcg aaccggtagg aaaaaagggt cttgaggaaa ctgccgaaac cgaacaggaa
     4921 cagaagctgc atgaagagcc ggagcttgtc cgtctcgaag agcaatcccg cggcccagag
     4981 ggcgcccagg ccggccaaga cggagagcgc ggggaggaac gtcatgagaa cgtcgacgag
     5041 gaactcgtcc caataggatt cgggtttttc ccggttgaat tccaccagcg gcattttgcc
     5101 gtaggtcatg gactggtggg aaagcgcggc gatgcgtttg gccggcagcg gatgggtgga
     5161 ctggatctcg taatagccgg cccacgggtt ccagaggtcc cattgcatgg ccgccaccac
     5221 gctggagagg ctgagtttgt tgccttgaga cagggacatg gcggccaggg cgagggcgcc
     5281 cttgcgttcg aagatgctga gggactggac ggtgtcgagc cggccggtgc gctcgggctg
     5341 ttttcccttt ttctcttcgc cgcccgccag gccgtaggcg atcttcacca gcgacatggc
     5401 cagttcgttg gggttgcggg tcttttcgcc ggcgaaccgg tcggcccaga actcgcgcac
     5461 gcgggaaaga tagaggacga tgaactcgga cacgaggtag atgaggtagg cggccagggc
     5521 ggcgacgtag agtggggcgg cgcctttggc ggaatccttg ccgcgcgagg aggaggcctt
     5581 ggcggccagg ttccataaca cgcggaaaag atagtagagg aggatgggga cggccgcggc
     5641 gagggtcatg acggcgatgt cccagtggac ggcgtggccg agttcgtgcg ccaccacggc
     5701 gttcagttcg ccttcggtga ggatctccat gaggccgcgg gtgacgacga tgcgggcgtt
     5761 gttgggcgtg tggccgtaag tgaaggcgtt gggggtgccg tcgttgatga tgccgaaacg
     5821 ggggattttg acattttcct ccgcgcagac gcgggagacg aattgctgca gggcgggggg
     5881 cagttcggtg aagtccaccc agcggagcga catggtccag cgcagggtcc agtccatgag
     5941 gaggggcgaa aggaggtatt gaaggaaaag gaatcccagg gccatcgccg ccgcggtgtg
     6001 cggttgccac cgggccactt cggccagaaa gaagaaggca aggaagacca gtccggagag
     6061 cacgccgaac acgccgaggg aacggagccc gaggttcggc agggtttgga gggggagggt
     6121 cgcggcggcc gcgagaggcc ggtggattcg gtcggccagg tcctggagtc cgaccgggag
     6181 ggcccgggcg actttgccgg atttgacggc ccggccgaga acggcgttta ttttttccaa
     6241 ttcgtccgcg tccaggaaaa cgccggaaca tttgggacag acatccacct tgatttcatt
     6301 ggagaagagg ccttgctcct gcaggaacgg gtcgcggcag cgggggcagg ggtggggcgt
     6361 ggaggtccga ccggccgggg cggtgatttc ctggtgcagg cgtttcggtt ccttgacgaa
     6421 aaaggagatt tcgttccggt cgaaccacac gccggcgcag gcgggacagg cgtcgatgag
     6481 cacgccctgc gggctgtaga cctcttccat gttttttttg catttgggac agttcatgac
     6541 gaacgctcct gtaaaaggga aaccgtccgg cggcg
//
LOCUS       DAOTFD010000141         6549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8406_length_6549_cov_5.055590, whole genome shotgun sequence.
ACCESSION   DAOTFD010000141 DAOTFD010000000
VERSION     DAOTFD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8406_length_6549_cov_5.055590"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="P5079_08195"
     CDS             complement(<1..677)
                     /locus_tag="P5079_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY29998.1"
                     /translation="MPGQILLDDEWHSLNFVIGRPFFSLFFAQGEGANAIPQNIYSGL
                     LLQTLGGSELLLRMPSLLLGLLALIVFPPLVEKAFNRVVAVVFAYLLAFSPVLTFYSR
                     LCRPYAAVCFFGFLSFLFMVLWLRRGKAAFLYGYAFSGFIAMYHHLYAAVPVLTPLLC
                     LSLLKLLPPGRRPAMKSAQMPSTRMMMRAAAILIVLGTVFILPPQLQNPWWQRIVGVG
                     GFDIPKFV"
     regulatory      1114..1180
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1212..>1476
                     /locus_tag="P5079_08200"
     CDS             1212..>1476
                     /locus_tag="P5079_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HRY29999.1"
                     /translation="MEKWIYLAVGSVAGGFARYLLSGAVHSKWGSGFPYGTLAVNLCG
                     CFLIGLLNGLAEEKFLLNPHARLLLMTGFCGAFTTFSTIMLESS"
     assembly_gap    1477..1486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1606..1950
                     /locus_tag="P5079_08205"
     CDS             1606..1950
                     /locus_tag="P5079_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HRY30000.1"
                     /translation="MRIPAEGKLLRIFIGEADKWRGQPLYEAIVHLAKKEGMAGATVL
                     KGFLGFGCKSHMHTAKILRLSEDLPIVIEIVDSEEKIAAFLPHLDEMVREGLITLEKA
                     NVILYRADGAKS"
     gene            2073..3296
                     /locus_tag="P5079_08210"
     CDS             2073..3296
                     /locus_tag="P5079_08210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY30001.1"
                     /translation="MPFQKLGLHPDLIRAVKALGYVEPTPIQREAIPLGMQGVDVMGC
                     AQTGSGKTAAFVLPILHRLLTHPKPGLRVLVLVPTRELADQVQSMFRECGRFTHFRVA
                     MVIGGVGYHDQIRAVKEGAQIMVATPGRLLDHLTQRAFNLTHVDHLVLDEADRMFDMG
                     FLPDIRSILSYVPHERTTLLFSATLVPEVERIAAFALKNPQRVEIARPGTTAEGISQV
                     LYPVVQSQKMDLLVTLLKTTEMRSVLIFCRTKFGADRLAHFLETQGHPAEVLHSNRTQ
                     GQRTTAMTNFRKGRSRILVATDIAARGIDVHGISHVINYDVPLHPEDYVHRVGRTARA
                     YGVGDAITLMDTEEQAAVTAIERFLGVVFPRAMVPDFPYRYPPRLVAPKYMTPWQRQA
                     GRRAKFSRRPGFRRS"
     gene            complement(3387..5684)
                     /locus_tag="P5079_08215"
     CDS             complement(3387..5684)
                     /locus_tag="P5079_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY30002.1"
                     /translation="MADPHALETDPVCGMKVDPARAAATYAHAGKEYYFCNPRCRDRF
                     RADPEKYRTPPSREIPGTGKNIPHVCPMHPEIRRLGPGDCPLCGMALEPASGAAEKNP
                     EGEEMSRRFWIGSALAVPVVFLAMGPMFFPSMATIVSPVFSHWGQFLLATPTVFWAGA
                     PFFWRAWRSIVSRRPNMFTLIALGTGAAYGYSVATLLFPGFLAQAVGPAHGALPVYFE
                     AASVITVLVLLGQVLELKARARTGAAVRALLELSPKSARVVRDGREFDIDLAEVRPGD
                     VLRVRPGEKVPVDGAVLEGQGGVDESLLTGESLPVGKKSGDRLIGGTVNGSGSFLFRA
                     EHVGRETVLARVVQLVEEAQRTRAPVQRLADEVSAYFVPSVLAVSLLTFGAWAFFGPQ
                     PRLLFALVNAVAVLIVACPCALGLATPMSVMVAVGRGAVAGVLVRDAEALELLGEADT
                     LLLDKTGTLTVGRPRLRTIVLAPGETETGVLRLAAGLEKASEHPLAGALTSAAAERGI
                     ALPAVSDFEARSGKGVVGRIDGRAVAVGSEALFDELGMNTALWRGKFEEARQAGQTVL
                     GVSVDGRWAGILGVADPLKEGTAEAVAHLKRLGLRVVMLTGDHRATAEIVARQSGVDE
                     VFAGLLPGQKKETVERFQAEGRIVIMAGDGVNDAPALAQAQVGMAMGSGADVALESAG
                     ITLLKGDLGGIVRALNLSRATRRNIRQNLFFAFLYNSLGVPVAAGILYPFFGWLLSPM
                     VASAAMSLSSVSVIANALRLKNVKL"
     gene            complement(5738..6487)
                     /locus_tag="P5079_08220"
     CDS             complement(5738..6487)
                     /locus_tag="P5079_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30003.1"
                     /translation="MKRFSSVPFVVWIGSALLLLGAAQGLALRAPRAWPPFDRPVLEK
                     YALSDMTGVLLGLRRAAADLAFIQMLQYYAGGHEEEHGEEDGHVHVGGEGSADLHPEI
                     RLTVFPSLREYVLRIASLDPYFHYAYLFGAGALAFNLNRSDEAFDVLRRGLERDPAFW
                     QFRLYVGAIAYRQSAQIEKVISLLEEALRYPDCPTMLQNILANIHVKIGNYQRAAEIY
                     LLMARTSRDQSYAEQAQKKLLELRRRHGVVS"
BASE COUNT         1365 a         2173 c         1906 g         1095 t
ORIGIN      
        1 acaaacttgg ggatatcgaa tcccccgacc ccgacgatcc tctgccacca cggattttgc
       61 aactggggcg gcaaaatgaa caccgtcccc aggacgatca ggatcgcggc ggccctcatc
      121 atcatgcgcg tcgacggcat ttgagcgctt ttcatggccg gccggcggcc gggcgggagc
      181 aacttcagca gcgacagaca gagcagcggg gtcaaaacgg gaacggcggc gtacagatga
      241 tgatacatgg cgatgaaccc ggagaaagcg tacccataca gaaaggccgc tttgccccgg
      301 cgcagccata acaccatgaa aagaaacgac aaaaaaccga aaaaacacac cgccgcgtac
      361 ggcctgcata atctcgaata gaacgtcagc acgggggaga acgccaacag ataggcgaag
      421 accaccgcga cgacgcgatt gaatgctttc tccaccagcg gcgggaagac gatgagggcc
      481 agaaggccca ggaggagcga gggcatccgc agaagcaatt ccgacccgcc caaggtctgc
      541 agaagaagac cactgtaaat attctggggg atcgcgttgg ccccttcccc ttgcgcaaaa
      601 aagagagaga agaaaggccg gccaatgacg aaattcaacg aatgccattc atcgtccaga
      661 aggatctggc ccggcaaatc atgaagccgc cagaaaacac cgatcgccat cgcggggatg
      721 aatggacatc cccataggaa atatcttcgg aaggggctat ccggcattgg ggaattcaca
      781 gcccgtttca tcggcatgtc ggggatcgac ggggccaaag tggatcgtcg gcttcaaaac
      841 catctcaaat tgacatccct cttgcaaaga cagtcatctt cgattcccgg gcgaacctcc
      901 cgggggcgtt tcctccgccc gcgcaggccc ctccgactta tttttaaacc atcccaagta
      961 tacgccgccg cccccgtttg tcaagaaaaa gtatcgtcgc tgtttgttga cggagtttta
     1021 ttaaaacctc tccccgacag accctgtttc ccaaaaatcc gccgacggcc ggcccgagaa
     1081 acgaacttgg catgacgcaa aaatttttgt ttactctctc acggcgatgg ggttcgccgt
     1141 aaccgccccg ctcgaggggg ctgatcactc ctaccgatca acgggtggga gtttttttat
     1201 gtgagggtcc catggagaaa tggatctatt tggccgtcgg cagcgtggcg ggcgggttcg
     1261 cccgctacct cctgtcgggg gcggtgcaca gcaagtgggg gtcgggtttc ccctacggga
     1321 cgctcgccgt caacctctgc gggtgtttcc tcatcggcct cctcaacgga ctggccgagg
     1381 aaaaattcct tttgaacccg cacgcccggt tgctcctcat gacgggtttc tgcggcgcct
     1441 tcaccacttt ctccaccatc atgctggagt cctcgannnn nnnnnngacg gagaaccgct
     1501 gctggccggc ctgaacgcgg ccctcagtct cgtcgtcgga tttgccttgt ttcgcctggg
     1561 aacgttcctg gtggagatca tttaactttc ggaggccttg aaatcatgcg aataccggcg
     1621 gaagggaagt tgctgcggat attcatcgga gaagccgaca agtggcgcgg ccagccgctg
     1681 tatgaagcca tcgtccatct ggccaaaaaa gaggggatgg cgggcgccac ggtcctcaaa
     1741 gggttcctcg ggttcggctg caagagccac atgcacaccg ccaagatatt gcggctgtcc
     1801 gaggacctgc ccatcgtcat cgagatcgtc gacagcgagg agaagatcgc ggcgttcctg
     1861 ccgcacctcg acgagatggt tcgggaaggg ttgatcaccc tcgaaaaagc caacgtcatc
     1921 ctgtaccggg ccgacggggc gaaaagctag gcgatttttc cgtctccccc cctctcgccg
     1981 ccaaacgggc ctctttattc ccctgcggca acttcaatat gttaatatcg ttgagaccga
     2041 agcctaaaat cgccgttcaa ggataagaca tcatgccatt tcagaaactg ggcctccatc
     2101 cggacctgat ccgcgccgtg aaggcgctgg gctacgtgga acccacgccc atccaaagag
     2161 aagccatccc cctgggaatg cagggggtgg acgtgatggg ctgcgcccaa accggcagcg
     2221 gcaaaaccgc ggcgttcgtc cttcccatcc tccatcgatt attgacccat cccaaacccg
     2281 gtctccgggt gctggtgctg gtgccgaccc gcgaactggc cgaccaggtt cagtcgatgt
     2341 tccgcgagtg cggccgtttc acacatttcc gcgtggccat ggtgatcggc ggcgtgggat
     2401 accacgacca gatccgcgcg gtgaaagaag gggcccagat catggtggcc acccccggcc
     2461 ggctcctcga ccatctgacg caacgggcct tcaacctgac gcacgtggac catctcgtgc
     2521 tcgacgaagc cgaccgcatg ttcgacatgg gattcctgcc cgacatccgc tccatcctga
     2581 gttacgtgcc gcacgaacgg accacgctgc tcttctcggc cacgctggtc cccgaagtgg
     2641 aacggatcgc cgcctttgca ttgaaaaatc cccagcgcgt ggagatcgcg cggccgggca
     2701 ccaccgccga aggcatcagc caggttctct atcccgtcgt ccaatcacaa aagatggacc
     2761 tcctcgtcac cctgctgaag acgaccgaga tgcgatccgt cctcatcttc tgccggacga
     2821 aattcggcgc ggaccgcctg gcccatttcc tggaaaccca gggacatccg gcggaggtgc
     2881 tgcattccaa ccgcacccag ggacaacgga cgaccgccat gaccaacttc cgcaagggcc
     2941 ggtcccggat attggtggcc accgacatcg ccgcgcgcgg gatcgacgtg cacggcatca
     3001 gccacgtgat caactacgac gtcccccttc acccggagga ttacgtgcac cgggtgggcc
     3061 ggacggcgcg ggcctacggc gtgggagacg ccatcacgct gatggacacc gaagaacagg
     3121 ccgcggtgac cgccatcgaa cggttcctcg gcgtggtttt tccccgagcc atggtccccg
     3181 atttcccata ccgctaccca ccccggctgg tggcgccgaa atacatgacc ccctggcaac
     3241 gacaggccgg ccgacgggcc aaattctcgc gccggcccgg attccgccgc tcctaataaa
     3301 aagatccggc gggagtcccg tcggatcttt ccattcgcac cgccctcctt tatcatcgac
     3361 gcccctcgcg gcggtttttt atcctcttac aatttcacgt tcttcagccg caacgcgttg
     3421 gcgatgacgc tgacggaact gaggctcatg gcggcgctgg cgaccatggg gctgagcaac
     3481 catccgaaga acggataaag gattcccgcc gccacgggca cgcccagact gttgtaaaga
     3541 aacgcgaaga agaggttctg gcggatgtta cgccgggtgg cccggctcag attcagcgcc
     3601 cggacgatcc ctcccaggtc gcctttcagc agggtgatac cggcgctctc caaggccaca
     3661 tcggccccgg accccatggc catccccacc tgagcctgcg ccagggccgg cgcgtcgttg
     3721 acgccgtccc ccgccatgat cacgatccgt ccttccgctt gaaaccgttc gaccgtctct
     3781 tttttctgac ccggcaaaag gccggcgaaa acctcgtcca ccccggactg gcgggccacg
     3841 atctccgccg tggcccgatg gtcgccggtc agcatcacca cgcgcaaccc caaccgtttc
     3901 aaatgggcca ccgcctcggc ggtcccctcc ttcaacggat cggccacgcc cagaatcccc
     3961 gcccaccggc cgtccaccga cacccccagg accgtttgcc cggcctgccg ggcctcctcg
     4021 aatttccccc tccagagcgc cgtgttcata ccgagctcat cgaacagcgc ctcgctcccc
     4081 accgccaccg cccttccgtc gatccgaccg accacccctt tgccggaacg ggcctcaaaa
     4141 tcagacaccg ccgggagcgc gatccccctc tccgccgcgg ccgatgtcaa cgcgcccgcc
     4201 aacggatgtt cgctggcttt ttccaggccg gcggccagcc gcagcacccc ggtctccgtc
     4261 tctcccggcg ccaggacaat cgtccggagg cgcggccggc ccacggtgag cgttccggtc
     4321 ttatccaaaa ggagcgtgtc cgcttccccc aggagttcga gcgcctccgc atccctcacg
     4381 aggacgccgg cgaccgcgcc gcgcccgacg gcgaccatca ccgacatggg cgtggccaac
     4441 cccaaggcgc agggacaggc cacgatcaac accgccaccg cgttgaccag cgcgaaaagg
     4501 agtctcggtt gagggccgaa gaaagcccac gcgccgaacg tcaacagcga aaccgccagg
     4561 acggacggga cgaagtaggc cgacacctcg tcggccagac gctggaccgg cgcgcgggtc
     4621 cgctgcgcct cctccacgag ctgaacgacc cgggccaaca ccgtctcccg gcccacgtgt
     4681 tccgcacgga acagaaaaga accggaccca ttgacggtgc cgccgatcag ccggtccccg
     4741 gacttttttc ccaccggcaa ggactccccg gtcagcaggg attcatccac gccgccctgt
     4801 ccttccagga ccgcgccgtc caccggcact ttctctcccg gccggacccg caggacatcg
     4861 cccggccgca cctccgccag atcgatgtcg aattccctcc cgtcgcgcac gacgcgcgcg
     4921 gatttcggcg acagctccag cagagcgcgg acggccgccc cggtgcgggc gcgcgccttc
     4981 aactccaaca cctgtcccaa cagcaccagc accgtgatca ccgaagccgc ctcaaagtag
     5041 accggcaaag cgccgtgcgc gggaccgacc gcctgggcca gaaaaccggg gaaaaggagc
     5101 gtcgccacgc tgtaaccgta cgccgcaccg gtccccaacg cgatgagcgt gaacatattg
     5161 ggtcgccgac tcacgatgga acgccacgcc cgccagaaaa acggcgcccc cgcccaaaac
     5221 accgtcggag tggccagaag gaattgcccc caatgggaaa aaaccggcga gacgatcgtc
     5281 gccatcgatg gaaaaaacat cggccccatg gcgagaaaaa cgaccggaac cgccagggcc
     5341 gatccgatcc aaaaacgacg gctcatctcc tccccttccg ggtttttttc cgccgccccc
     5401 gaagcgggct ccagcgccat gccgcagagg gggcagtccc cgggcccgag ccgacggatt
     5461 tccggatgca tgggacaaac gtgggggatg tttttccccg tgccggggat ttctcttgaa
     5521 gggggggtgc gatatttttc ggggtcggcc cggaatctat cgcgacagcg cgggttgcag
     5581 aaataatatt cttttccggc gtgcgcatag gtcgcggcgg cgcgggcggg gtccaccttc
     5641 atcccgcaaa cgggatcggt ctcgagcgcg tgtgggtcgg ccatgggaga gcctctggaa
     5701 cgtcttcgcg tgcggatctt aaagaatgcg gggaatctta ggatacaacg ccgtgtcgac
     5761 ggcgaagttc caggagtttc ttctgggcct gttccgcgta ggattggtcg cgggaggtgc
     5821 gcgccatgag gagatagatt tccgcggcac gttgataatt cccgattttt acatggatgt
     5881 tggcgaggat gttctgcagc atggtggggc aatcgggata acgcagggcc tcttccagga
     5941 gggagatcac tttctcgatc tgcgccgact ggcggtaggc gatggcgccg acataaagac
     6001 ggaattgcca gaaggccggg tcccgttcca atccgcggcg caagacatcg aaagcctcgt
     6061 cggagcggtt gagattgaac gccagcgctc ccgcaccgaa cagataggcg taatgaaagt
     6121 aggggtccaa ggaggcgatg cgcaggacat actcccgcag ggacggaaaa accgtcaaac
     6181 ggatttccgg gtggaggtcc gccgagcctt ccccgcccac atggacgtgt ccgtcctcct
     6241 ccccgtgctc ctcctcatgg ccgccggcat aatattgaag catctggatg aacgccagat
     6301 cggcggccgc gcggcgcagt cccaggagga cgccggtcat gtcggacagg gcatattttt
     6361 ccaagacggg acggtcgaag ggcggccagg cacggggggc acggagggcc aggccctggg
     6421 cggctcccaa caacaggagc gccgacccga tccagacgac aaaagggacg gacgaaaaac
     6481 gtttcatgac ggcggctcag aactcttttc gggaaaacaa cgccagggcc aggcagaaac
     6541 aggccgcgg
//
LOCUS       DAOTFD010000142         6478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8542_length_6478_cov_3.793866, whole genome shotgun sequence.
ACCESSION   DAOTFD010000142 DAOTFD010000000
VERSION     DAOTFD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8542_length_6478_cov_3.793866"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="P5079_08225"
     CDS             <1..1567
                     /locus_tag="P5079_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30004.1"
                     /translation="LFIANIDDNSVHMVDVARMEILAKITGVSCPQRLIIHPGRRWVY
                     VIDNYLDRFHVIDRVHRRLVKSIFPGDDPSGIAFDSRGKCYLANFWSNDFAVVDLETE
                     EVEERVALRGAAPHSLWFDPHHRKMFVTNGSSNGILVMDPDTGNVVDQLVMPDGAYGG
                     PLTVNRARGRLYVADDWRSGVGIYPLDTADPGYQTPHHERKFISLPGAHPSGIAAAPG
                     DRLCVPFVKGDRLTLALVDQEQEAVEAEIDLGAGLQTGGVAVNERKKVAYVADYSGAR
                     LIAVDLKRKQVCGAVSLERQPSAVAVHPESGLIYVCNPGSNSVAVVADDPLETLGFID
                     VGAAPSAIAFSPSKARLYVTNSGDGTVSVVNELSHRVVETVPVGEDARQLVVENDNLY
                     VASAARGRISTLRDTFRLAPSFNNDRSASDTAFRFREAYGYPNPARGTNPVLRAQVGL
                     ADAVQFSIYDVSGALVHEAAAPAAVLTENGYVYDYAWDVSSVASGVYLVSLCAKKAGE
                     TDIRKTFKVAVLK"
     gene            1564..2532
                     /locus_tag="P5079_08230"
     CDS             1564..2532
                     /locus_tag="P5079_08230"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30005.1"
                     /translation="MRDTKYEIRNTKKCLTITAAISAACFVFCISDFVFAAPTGATLL
                     KIGVGARAVGMGSAYTAVADDVTAIHWNPAGLSRLSQRQLAAMHSEWLSESRYDFIGV
                     AVPSAHGSWGASALYLSQGGLERRDLSGTAGGRFNAYDAAAAVSYSRALSARLSAGAA
                     VKYVRQGIDAAASSGYAADVGLQMRLRRGVRAGLAVQNLGSTLRFESEEYRLPLSVSG
                     GVAVSFLKGILLAADVRQAIYESRTEFNLGTEYTHGLLSLRAGYQALLAGRETLSGKS
                     AGFSAPFAGGMGGGIGFRLSSYRLDYALVPYGELGSAHRLSLSTRF"
     gene            2620..2919
                     /locus_tag="P5079_08235"
     CDS             2620..2919
                     /locus_tag="P5079_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30006.1"
                     /translation="MDTILVVFSALSLLALGIITVYLVRTLDQVRRTARSLEILSDNL
                     NGELSKVQKVTAAAAEAADLFTGGVGKSITLAAAFVRGFLKMKRKFSGTADEAGK"
     gene            2957..3238
                     /locus_tag="P5079_08240"
     CDS             2957..3238
                     /locus_tag="P5079_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRY30007.1"
                     /translation="MSENNKGGDTLIAFLLGGLVGAGIAFLFAPRAGKETRRRLQHWL
                     EDMEDKGQDLLEEGRELLQEGKEVVQEKTEKIKRVIDSGRKAWDEHTKN"
     gene            3268..3810
                     /locus_tag="P5079_08245"
     CDS             3268..3810
                     /locus_tag="P5079_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY30008.1"
                     /translation="MSAEPKNAGPGPWSRNLWRRLFRDEVFRDQMRFFRKVILFRGLS
                     DRLLSRLVHTIMEKTYAEKDLIFQEGDVGRAMFIVAEGRVRLFQNGKKNGPPETIAEI
                     GPGEFFGEMVLLDELPRSASAEALESTRLYILYKSNFDGLLTDAPDVASKVLHTLARL
                     LSARLRRENFNSTSVNYPGR"
     gene            3833..4927
                     /locus_tag="P5079_08250"
     CDS             3833..4927
                     /locus_tag="P5079_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY30009.1"
                     /translation="MNESKLIRFLLVALGVFLAAKFFYAVRVVLAPFVLAAVFAFVMN
                     PVVRFMELRGVRRSSAVMLLYAALTVGFVALAYWAAVVLWADLPKLRYQLPAYLQQFN
                     AAAVQLGGLLSEKCPWLVDKKAVEHGASQLVLYAQNALQAAPHYVATIATIAIYFILV
                     PFVSYYLLKEGKTFFQGLLNACPGRWMEKYLSLFYEIDAVLGNYLRGIFFEAILVGVL
                     ATVALFLLGIPYAALVGAVVCVGNIVPYLGPILGSAVGAAVAFFQFNNVVAPIKVLLA
                     FAAIKFVDDWFFQPFIMRRSVDLHPVIVILSLLCGGELAGFWGLLLGVPVVCIVHEVA
                     KVFSAWSLAESRTRKMPREVWEAAGRPWIV"
     gene            5306..5381
                     /locus_tag="P5079_08255"
     tRNA            5306..5381
                     /locus_tag="P5079_08255"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5339..5341,aa:His,seq:gtg)
     gene            complement(5360..>6478)
                     /locus_tag="P5079_08260"
     CDS             complement(5360..>6478)
                     /locus_tag="P5079_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRY30010.1"
                     /translation="GSCPVLGYNYDSEKKTLEVNKEEADQIVDIFQTYLKNKSLNLTA
                     RSINQKGYHMKTWITFGKKLKRGGRPFNKANLHWLLRNPLYLGKIRYKGHLYDGEHQG
                     FLSEKLFNAVNKILKGNQNGTVDRERRKDTGHNFMLKGIIRCGNCGSAMTPTFAYNKN
                     KKTFHYYRCVSALKGDKECTGKYVSAPELERFVIDRIKFLADNKEVMEGIITKSRDSN
                     IEILPIKLREKSNLAAEMGKVDLNAQNLVGILAEEGPDSQRKKYVLEELDKINASKIQ
                     LTERWEKLNKEIQALEERLIEADVIRNNLKNFQNVFQRLTPMEQKELVQLMVKEVVIN
                     HTNSQVQLTLRALPEMDWGVAQSGQCSISVKNGVGNGD"
BASE COUNT         1109 a         1975 c         1913 g         1481 t
ORIGIN      
        1 actgttcatc gccaacatcg acgacaacag cgtgcacatg gtggacgtgg cgcgcatgga
       61 gatcctcgcc aagatcaccg gcgtctcctg tccccagcgc ctcatcatcc atccgggccg
      121 ccgctgggtc tacgtcatcg acaactacct cgaccgcttc cacgtgatcg accgcgtcca
      181 tcgccggctc gttaagtcca ttttcccggg ggacgacccg tcgggcatcg cgttcgattc
      241 ccgcggcaag tgttatctcg ccaatttctg gtccaacgat ttcgccgtgg tggacctgga
      301 gaccgaggag gtggaggagc gggtggcgct ccggggggcc gcgccgcaca gcctttggtt
      361 cgatccccac caccgcaaga tgttcgtcac caacggctcc agcaacggca tcctggtgat
      421 ggacccggac accggcaacg tggtggacca gctcgtcatg ccggacggcg cctacggcgg
      481 cccgttgacc gtgaaccgcg cccgcggccg gctctatgtg gcggacgatt ggcgcagcgg
      541 cgtggggatc tatccgctcg ataccgccga tcccgggtac cagacgccgc accacgaacg
      601 caagttcatc tctttgccgg gggcgcaccc gtccggcatc gccgccgccc cgggggacag
      661 gctctgcgtg ccgttcgtga agggggaccg gctcaccctg gccctcgtgg atcaggaaca
      721 ggaagccgtg gaagccgaga tcgatctggg ggcgggcctg cagaccggcg gcgtggcggt
      781 gaacgaacgg aaaaaggtgg cgtacgtcgc cgactattcg ggcgcgcggc tcatcgccgt
      841 ggatttgaaa cggaaacagg tctgcggcgc cgtctccctg gaaaggcagc ccagcgccgt
      901 ggccgtccac ccggaatcgg gactcatcta cgtctgcaat cccggctcca attccgtggc
      961 cgtggtcgcc gacgatcctc tggaaacgct ggggttcatc gacgtcggcg ccgcgccctc
     1021 ggccatcgcg ttctctccgt ccaaggcgcg cctgtacgtc accaactccg gcgacggcac
     1081 cgtttccgtg gtcaacgagc tgagccaccg cgtggtcgag accgtccccg tgggcgagga
     1141 tgcccgccag ctcgtggtgg agaacgacaa tctctacgtg gccagcgccg cccgcggccg
     1201 catctccacc ctgcgggaca ctttccggct ggccccgtcg ttcaacaacg accggtccgc
     1261 ctccgacacc gccttccgtt tccgtgaagc ctacggctac cccaacccgg ccaggggaac
     1321 caaccccgtt ctgcgcgcgc aggtgggcct ggccgatgcg gtccagttct ccatctacga
     1381 cgtttccggg gccctggtcc acgaagccgc cgccccggcc gccgtgttga cggaaaacgg
     1441 gtatgtgtat gactacgcgt gggacgtctc ttcggtggcc tcgggcgtgt atctggtgtc
     1501 cctctgcgcc aaaaaggccg gcgaaacgga catacggaaa acgttcaaag tggcggtttt
     1561 aaaatgagag atacaaaata cgagatacga aatacgaaaa agtgcctgac gatcaccgct
     1621 gcgatatcag cggcctgctt cgtattttgt atttcggatt ttgtctttgc cgctccgacc
     1681 ggcgccactc tcctgaagat cggcgtgggg gctcgggccg tgggtatggg gtccgcctac
     1741 accgccgtgg ccgacgacgt cacggccatc cactggaatc ccgcggggtt aagccggctg
     1801 tcccaacggc agttggcggc catgcattcc gagtggctgt ccgagtcgcg gtacgatttc
     1861 atcggggtgg ctgtcccgtc cgcccacggt tcctgggggg ccagcgcctt gtatctgtcc
     1921 cagggcggcc tggaacgccg cgacctctcc ggaacggccg gcggccgctt caacgcgtac
     1981 gacgccgcgg ccgcggtgtc gtattcccgc gccctgtccg cccgtttgtc cgccggagcc
     2041 gccgtgaaat atgtccggca ggggatcgat gccgccgcct cgtcgggcta cgccgccgat
     2101 gtgggcctgc agatgcgcct gcggcgcggc gtgcgcgcgg gattggccgt gcagaatctc
     2161 gggtccaccc tccgtttcga gtcggaggaa taccgcctgc cgctgagcgt ttccggcggc
     2221 gtcgccgttt ctttcttgaa ggggatcctc ctggcggcgg acgtgcgcca ggcgatttat
     2281 gagagtcgca ccgagttcaa cctgggcacc gaatacaccc acgggctcct ttccctgcgg
     2341 gccggctatc aggccctcct cgccggccgg gagacgctct ccggaaaatc cgccggtttc
     2401 tccgcgccgt tcgccggtgg aatgggcggc ggcatcgggt tccgcctgtc gtcctaccgg
     2461 ctggattacg ccctggtgcc ttacggggaa ctcggctcgg cgcaccgcct ctccctctcc
     2521 acgcggtttt aaacggctgt ctcttgctcc ctccgaccgg atataggata atgaacttat
     2581 ccgtccggtt ttttcatacg ccctaaagga gtcgctctca tggatacgat acttgttgtt
     2641 ttttcggccc tctccctgct ggccctggga atcatcaccg tttatctcgt tcgcacgctc
     2701 gaccaggtcc gccgcaccgc gcgctcgctg gaaatattgt ccgacaatct gaacggggaa
     2761 ctgtccaagg ttcaaaaagt cacggcggcc gccgcggagg cggccgacct cttcacgggc
     2821 ggggtgggaa aatccatcac cttggccgcc gcgttcgtgc ggggtttcct gaagatgaag
     2881 agaaagtttt ccgggacggc cgacgaggcc gggaaataac gcttttagat ttggaatatc
     2941 acagaggagg ttcgtcatga gcgagaataa caagggaggg gacaccctca tcgcgttttt
     3001 gttgggtggg ttggtgggcg cggggatcgc gttcctgttt gcgccgcggg cgggcaaaga
     3061 gacgcgccgc cggctgcagc attggctgga agacatggag gacaagggcc aggaccttct
     3121 tgaagaggga cgtgaactcc tgcaggaagg caaagaggtc gttcaggaaa aaactgagaa
     3181 gataaagcgc gtcatcgaca gcggccgcaa ggcctgggac gagcatacga agaattgatt
     3241 tccctgtaag accccgcgac gtcttccatg agcgctgaac ccaagaacgc cgggcccggc
     3301 ccgtggtccc ggaacctgtg gcggcgtctt ttccgcgacg aggtttttcg ggaccagatg
     3361 cggttcttcc gcaaagttat ccttttccgc ggcctttccg accgcttgtt gtcccgtctg
     3421 gtgcacacca tcatggaaaa gacctacgcc gaaaaggacc tcatcttcca ggagggcgac
     3481 gtggggcggg ccatgttcat cgtggccgaa ggacgcgtgc ggctctttca gaacgggaag
     3541 aagaacggcc cgccggaaac catcgccgaa atcgggccgg gcgaattctt cggggagatg
     3601 gtcctcctcg acgagcttcc ccggtccgcc tccgccgagg cgctggagtc cacccggctt
     3661 tatatcctgt ataaatccaa tttcgacggc cttctcaccg acgctccgga cgtggcttcc
     3721 aaggtgctcc acaccctggc ccgcctcctc agcgcccggc ttcgccggga aaatttcaac
     3781 tccacctccg tgaactaccc cggccggtaa ccgcgttcct tcccgttccg caatgaacga
     3841 atccaagctc atccgtttcc tcctcgtggc gctcggcgtt ttcctggcgg cgaaattctt
     3901 ttatgccgtg cgggtggtgc tggccccgtt cgttttggcc gccgttttcg ccttcgtgat
     3961 gaaccccgtg gtgcggttca tggaactgag gggagtccgt cgctcctccg ccgtcatgct
     4021 cctctacgcg gcgttgacgg tggggtttgt ggcgctggcc tattgggccg ccgttgtttt
     4081 gtgggccgac ctgcccaagc tgcggtatca gttgccggcc tatttgcagc agttcaacgc
     4141 cgccgcggtc caactcggcg ggctcctgtc cgaaaagtgc ccgtggctgg tggacaaaaa
     4201 agccgtggaa cacggggcca gtcagctcgt cctctatgcg cagaacgctc ttcaggcggc
     4261 gccgcattac gtggccacca tcgccaccat cgcgatctat ttcatcctgg tgccgttcgt
     4321 ctcctattac ctgctcaaag agggtaaaac gttttttcag gggctcctga acgcctgccc
     4381 cggccggtgg atggagaaat atctcagtct tttctatgag atcgacgcgg tgttggggaa
     4441 ttatcttcgg gggatctttt tcgaggccat cctcgtcggc gttttggcca ccgtggccct
     4501 cttcctgttg ggaattccgt acgccgcgct ggtgggagcg gtggtctgcg tcggcaacat
     4561 cgttccgtat ttggggccca tcctgggcag cgcggtcggc gcggccgttg ctttttttca
     4621 gttcaacaac gtcgtggcgc ccatcaaggt cctcctcgcc ttcgctgcca tcaagttcgt
     4681 ggacgactgg ttctttcaac cgttcatcat gcggcggagt gtggaccttc acccggtcat
     4741 cgtcatcctg tcgctgttgt gcggcggaga gttggccggt ttttggggcc ttttgctggg
     4801 cgttcccgtg gtctgcatcg tccacgaagt ggccaaggtt ttttccgcct ggtccctggc
     4861 cgaatcccgc acgcgaaaaa tgccccgaga ggtgtgggag gccgccggcc gcccgtggat
     4921 tgtctgaaaa aattcagtat tatttctctt ggcctactcc ctgagcgcta ggccgctagg
     4981 catggtaaag ggactgcgaa gggtgcgaca gaaggcgggt atcgccctga gagatttcaa
     5041 gcactttgtg cgaacctcag gcccctgcgc tccgagcagg ggtcagcggt gtccgcgcgg
     5101 tggcggcgta gctttacggt gggtatagct cagcggttag agcgtccgca gtggaacgcc
     5161 ggccactttc aaggggccgt gcacagatag gcaggcatcc tctccacaga ggcaggggag
     5221 ccgacccgtg gcccggttcc gggcccagcg tttctatact cggcacgcgt tactggattt
     5281 tttgttactc agcactgtct ttacggtggg tatagctcag cggttagagc gtccggctgt
     5341 ggcccggaag gtcgtgggtt caatccccat tacccacccc atttttgacg ctaatcgaac
     5401 actgtcctga ctgggcaact ccccagtcca tttcgggcag ggcgcgtaga gtgagctgga
     5461 cttggctatt ggtgtggttg atgacgacct ctttcaccat tagctgaacc agctcctttt
     5521 gttccatcgg tgtcagtcgt tggaagacgt tctggaagtt cttgaggttg ttccggatca
     5581 catccgcttc gatcaatctc tcctcgagtg cctgtatttc tttgttgagc ttctcccatc
     5641 gttccgtcaa ctgaatcttg ctggcattga ttttgtccaa ttcttccaaa acatactttt
     5701 tccgctgaga atcgggcccc tcttcagcca atattcccac caaattttgc gcgtttaggt
     5761 cgaccttgcc catctctgcg gccaggttgc tcttctcgcg gagttttatg ggcaggatct
     5821 cgatgtttga atcccggctc tttgtgatga tgccttccat gacctcttta ttgtcagcca
     5881 gaaattttat tcgatcaata acaaaccgtt ccaattcagg cgctgaaaca tatttcccgg
     5941 tgcattcctt gtcccctttt agggcggaca cacacctgta ataatgaaat gtcttcttgt
     6001 tcttgttata ggcaaacgtg ggcgtcatgg cgcttccgca gttgccacat cgaatgattc
     6061 ccttcaacat gaaattgtgg ccggtgtctt ttcttctttc ccgatctacc gtcccattct
     6121 ggttaccttt aagtatttta ttcaccgcgt taaaaagttt ttcgcttaag aagccttggt
     6181 gctctccatc atagaggtgg cccttatacc gaattttccc gagatagagg ggattcctga
     6241 gtagccagtg caaattggct ttattgaaag gccgcccgcc tcttttgagt tttttgccaa
     6301 aggtgatcca cgttttcatg tgataacctt tttgattgat gctacgggct gtcaagttga
     6361 gcgacttatt tttaaggtag gtctgaaata tgtcaacgat ctggtccgcc tcttctttgt
     6421 tgacttcgag agtttttttc tctgaatcgt agttgtatcc caacacagga cagcttcc
//
LOCUS       DAOTFD010000143         6447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8591_length_6447_cov_4.248436, whole genome shotgun sequence.
ACCESSION   DAOTFD010000143 DAOTFD010000000
VERSION     DAOTFD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8591_length_6447_cov_4.248436"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="P5079_08265"
     CDS             <1..1445
                     /locus_tag="P5079_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRY30011.1"
                     /translation="HGYGLVMGPVNEVTSEGLPLLWARDIPPKHDPEFPIVRPEIYFG
                     ERANEYIIVNTKSKELDYPAGDQNVYTHYQGAGGVPLSGFLRKVAFVFRFGTLKILLS
                     RDITPESRLLFRREITERLRAIAPFFLYDNDPYPVLADGRIFWIVDGYTWSDRFPYAQ
                     PIRGRLNYIRNSVKAVVDAYNGTVSFYLADAEDPVVQCYNRVFPGLLKPLAEMPASLK
                     SHLRYPQDLFEVQSAVYARYHMTNPQVFYNQEDLWTTPDELVEGNETPMEPYYTIMRL
                     PGEAKAEYVLLLPFTPRQKSNLIAWMAARSDDPHYGQILVYRFPKEKLIYGPMQIAAR
                     IDQDAQISQQFTLWGQRGVQVLRGNLLVIPVKNSLLYIQPVYLQAERGKIPELRRVIV
                     AYEERIAMRETLNEALQDIFSGWTPAPASPTTAGPRAPPAADLSGIGKDAWTIFQRGQ
                     ERLQKGDWTGYGRAMEELADVLRRLKDSSR"
     gene            1567..1881
                     /locus_tag="P5079_08270"
     CDS             1567..1881
                     /locus_tag="P5079_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRY30012.1"
                     /translation="MSLLLEFSLTPLGKGESVGKHVARVLDIVDKSGVPYRVHSMGTV
                     LEGDWETVFGVVKKCFRRMQKDCPRVILTLKADGRKGRAGRIAGKVAGLEKRLGRRLT
                     KD"
     gene            1901..2395
                     /locus_tag="P5079_08275"
     CDS             1901..2395
                     /locus_tag="P5079_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY30013.1"
                     /translation="MTLYLIDGYNLIRSADFLAAGPLRDQRERLIRLIEQQHPQGSAR
                     HRVTVVFDGRDDVSSPSRAGSVEVLFSRGRHGGEPLDAGTADALIKERVDRLAHPRDS
                     VVVTDDKALQKWVRGAGAKVLSCRQFLAAAKKNAPAAGPAPNPPAEDGEDITEELKRL
                     WNLR"
     gene            2392..3105
                     /gene="sfsA"
                     /locus_tag="P5079_08280"
     CDS             2392..3105
                     /gene="sfsA"
                     /locus_tag="P5079_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRY30014.1"
                     /translation="MKFQPPLREGVLIRRYKRFLADVRFSDGETVTALCPNTGSMKSC
                     CEPGRTVLLSYHPERGRKYPYTWEMIRMERSWVGVNTALPNSLVERAVRYGMIPELIS
                     YTKVRREVRTGESSRLDFVLEGAPGLCYMEVKNVSLEERGEARFPDAVTERGRRHLNE
                     LMRLQREGHRAVMFFLVQRDDVSLFRPADDIDPAYGQALRNAAAAGVEVLAYRAQVSP
                     QGLRWGELIPKDLSLPPSH"
     gene            3122..3409
                     /locus_tag="P5079_08285"
     CDS             3122..3409
                     /locus_tag="P5079_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRY30015.1"
                     /translation="MFDKMKQLMELQKKAREMQAAMDALRVEKTGADGKIRLVMNGNF
                     RVESISIDESFLSPSQKRPLEEALTRLVTETAEEIRQRSASQALGMMKGVL"
     gene            complement(3433..4557)
                     /locus_tag="P5079_08290"
     CDS             complement(3433..4557)
                     /locus_tag="P5079_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY30016.1"
                     /translation="MIKKITLTITAFLLISPLFAAPKVVVLAVDGASENVIRDLSRRK
                     SIPHLTKLFQQGWYGSINMGGGLSPTKWMTVFSGCPVGIHGVASWEINGESLSSNHCR
                     VKRVWEILSDHGYHVGVFQVLATSPADPIRGVLLTDRWTSFDRPAMERGFPPEILPRL
                     ARHFENNVRGLATRDEVVLMRTQETAALQLLLELDEEEAFRLIVFYLRSVDEASHGVW
                     KKDTEIVGGDLRVKPGGELEKAYLHADAAAERVMGLASPEDYVFFLSDHGFSDDLFYS
                     EEVPAGRGRHSAEGIFFLKGPGIRPGRLSKTIDGLALVPTMLDIFQVPASEQMPGESL
                     LSAGKKLLRVKTYTKTISPHTPAPYSAEERKKLEQLNYLK"
     gene            4573..6246
                     /locus_tag="P5079_08295"
     CDS             4573..6246
                     /locus_tag="P5079_08295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30017.1"
                     /translation="MKKRLHPLLLAFLLPVALGASFVGRPCFVDDSYHLLMAKGLLEH
                     PLRPYDFKADDAGPNNPGWEYGRPPRMVNPPLHHYLLALFLKAGGDRIWLARLGFLLL
                     SGLSALLIFLLSQRFMVPPLPATVFAVLTPAFWLSSYSLLIDPTMLLFFLAALLFWID
                     GLRKNSTPLLVAAGLSMGAACLTKYTAALVVPLAFLWWLLRPREERRFRPLLFLGLPF
                     LLLGLWSLWNVHTYGAPHLLASSKRVVQEFHWTNLLVFLTFFSGVFLFPLFAWLWDLR
                     LARRPFLAAAGLTLILFALLAGPWGGFSMDQALLVSLLAGGGVLFFQLVAQTVVARAL
                     PSDVFLLLWLFLNSIQMIFIMGWFAGRYYLTVLPPAVFLFYRALQRRWPHAPGALRRF
                     STLIEALLFLSGLALATADHLQATARRRIVDELERDGWTDAGRRCFALGDSFTGDYLK
                     AAGWTTAFPETRFQHGDLVLKNDVVMPAWWFRPEKDRFRPLKIYEYASRFPLRVMDNQ
                     GAAGFYASVWGALPFSFSRSPLERYVLLEYAGEAPSESPRRALPHRAGI"
     gene            6262..>6447
                     /locus_tag="P5079_08300"
     CDS             6262..>6447
                     /locus_tag="P5079_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30018.1"
                     /translation="MENTPSGNSHTILIADDDRVAQQTLETLLTGIGYRVLVAHNGEE
                     ARDRIREFRPDLCVLDID"
BASE COUNT         1220 a         2199 c         1812 g         1216 t
ORIGIN      
        1 cgcacgggta cggcctggtg atgggcccgg tcaacgaggt gacgagcgag ggactgcccc
       61 tcctgtgggc gcgggacatc ccgccgaaac acgaccccga gttccccatc gtgcggccgg
      121 aaatctattt cggcgaacgc gccaacgagt acatcatcgt caacaccaaa tccaaggagc
      181 tcgattaccc cgccggggac cagaacgtgt acacgcacta ccagggcgcc ggcggcgttc
      241 ccctgagcgg cttcctgcgc aaagtggcgt tcgtgttccg cttcggcacg ctaaagatac
      301 tcctctcgcg cgacatcacc ccggaaagcc ggctcctttt ccgacgggag atcaccgaac
      361 gcctccgcgc catcgcgccc tttttcctct acgacaacga tccctacccc gtcctggccg
      421 acggacggat cttctggatc gtggacggct acacctggtc cgaccggttc ccctacgccc
      481 agcccatccg gggccggctc aactacatcc ggaactccgt caaggcggtg gtggacgcct
      541 acaacggcac cgtctcgttc tatctggccg acgcggagga tcccgtcgtg caatgctaca
      601 accgcgtctt cccaggcctc ctcaagccgc tggccgagat gccggcgtcc ctgaaaagcc
      661 acctgcgcta cccgcaggat ctgttcgaag tgcagtccgc cgtgtacgcc cgctaccaca
      721 tgaccaaccc ccaggtcttc tacaaccagg aggacctctg gaccacgccg gacgaactcg
      781 tcgaggggaa cgaaacgccg atggaacctt attacaccat catgcgcctt ccgggcgagg
      841 cgaaagcgga gtacgtcctc ctgcttccat tcaccccgag acagaaaagc aacctcatcg
      901 cgtggatggc ggcccggagc gacgacccgc actacggcca gatcctggtg taccggttcc
      961 ccaaggaaaa actgatctac ggccccatgc agatcgcggc gcgcatcgac caggacgccc
     1021 agatctccca gcagttcacg ctctggggcc agcgcggcgt gcaggtgctg cgcgggaacc
     1081 tgctggtgat cccggtcaaa aattccctgc tctacatcca gccggtgtat ctgcaggccg
     1141 aacgcggaaa aatcccggaa ctccgccgcg tcatcgtcgc ctacgaggag cgcatcgcca
     1201 tgcgcgaaac gctgaacgag gccctccagg acatcttctc cggctggacg ccggcgccgg
     1261 cgtcgcccac aaccgccggc ccccgcgccc cgccggccgc cgatctgtcc gggatcggaa
     1321 aagacgcctg gacgattttc caacgcgggc aggaacgcct gcagaagggc gattggaccg
     1381 gatacggccg cgccatggaa gaactggccg acgtcctccg gcggctcaag gactcctccc
     1441 gctgaaagac ttcgtctttc ttatgcaagg gttccagaaa aaaaagcatc gcttcttcca
     1501 gcgaacggat cggcgcgcgg cggcgtttgt cgatgtgatc cgaatgcgta aaccctcaga
     1561 ggatttatga gcctgcttct cgaattcagt ctcacccccc tcggcaaagg cgaaagcgtg
     1621 ggcaagcacg tcgcccgcgt gctggacatc gtcgacaaga gcggcgtccc ctaccgggtg
     1681 cattccatgg gaaccgtcct ggaaggggac tgggaaacgg ttttcggcgt ggtgaagaaa
     1741 tgtttccgcc ggatgcagaa agactgcccc cgcgtcatcc tcaccctcaa agcggacggc
     1801 cgcaaaggac gcgccggccg gatcgccggc aaagtggccg gcctggaaaa acgactgggc
     1861 cggcgactca cgaaagattg acgatccctc ccgatcgcgc gtgacgctct acctcattga
     1921 cggttataac ctcatccgtt cggcggactt tctcgccgcc gggccgctcc gcgaccagcg
     1981 ggaacggctg atccgcctca tcgaacaaca acatccgcag ggaagcgccc gtcaccgcgt
     2041 gaccgtggtc ttcgacggcc gggacgacgt ctccagcccc tcccgggccg ggtccgtgga
     2101 ggtgctcttt tcccgcgggc ggcacggcgg ggagcccctg gacgccggca ccgccgacgc
     2161 gctcatcaaa gaacgcgtgg accgcctcgc ccacccgcgg gactcggtgg tggtcaccga
     2221 cgacaaagcc ctccagaagt gggtgcgcgg cgccggagcg aaggtcctct cctgccggca
     2281 attcctcgcc gccgcgaaaa aaaacgcccc cgccgccggg ccggcgccga acccgccggc
     2341 cgaagacggc gaggacatca ccgaggagtt gaaacgcctg tggaacttga gataaagttc
     2401 caaccgccgc tccgggaagg cgtcctgatc cgacgctaca aacgttttct ggcggacgtg
     2461 cgtttcagcg acggcgaaac cgtcaccgcc ctgtgtccca acacggggag catgaagtcc
     2521 tgctgcgagc cgggccggac cgtgttgctg agttatcacc cggaacgggg ccgaaaatat
     2581 ccttatacct gggagatgat ccgcatggaa cgctcctggg tcggcgtcaa caccgccctg
     2641 cccaattcgc tggtggaacg ggcggtgcgt tacggaatga ttccggaact gatcagctac
     2701 accaaagtcc gccgcgaggt acgtaccggc gagagcagcc gtttggattt cgtcctggaa
     2761 ggcgcgccgg gcctttgcta tatggaagtg aagaacgtga gcctggaaga gcgcggcgaa
     2821 gcccggttcc ccgatgcggt cacggaacgg ggacggcggc acctgaacga actgatgcgg
     2881 ctgcaacggg aagggcaccg cgccgtgatg ttcttcctcg tccaacggga cgacgtctcc
     2941 ctcttccggc cggccgacga catcgacccg gcctacggac aggccctccg aaacgcggcc
     3001 gccgccggcg tggaggtcct ggcgtaccgg gcgcaggtct ccccccaggg gctccgctgg
     3061 ggcgaattga ttcccaaaga tttgtctttg cccccatccc attaaaaaag acggtgaccc
     3121 catgttcgac aaaatgaaac agttgatgga acttcaaaaa aaagcgcggg agatgcaggc
     3181 cgccatggac gccctccggg ttgaaaaaac cggcgccgac ggtaaaatcc ggctggtgat
     3241 gaacggcaat ttccgcgtgg aatccatttc cattgacgaa agctttctct ccccctcgca
     3301 aaaacggccc ctggaagaag ccctcacccg cctcgtcacc gaaaccgccg aagagatccg
     3361 ccagcgatcg gcctcccagg ccctggggat gatgaagggg gttttgtaaa ggagaaaccg
     3421 gccggaaccg ctttatttca gataattcag ctgttccagc tttttccgct cttcagcgct
     3481 gtagggcgcc ggcgtgtggg ggcttatcgt tttcgtgtat gtcttgaccc ggagcaattt
     3541 tttgcccgcc gacaagagcg attcgcccgg catctgctca gaagccggaa cctggaatat
     3601 gtccagcatc gtcggaacaa gagccaaacc gtcaatcgtc ttggaaagac gccccggcct
     3661 gatccccggc ccctttaaga aaaatatccc ctccgccgaa tgccgcccgc ggccggcggg
     3721 gacttcttcc gaataaaaaa gatcgtccga aaacccgtgg tcggacaaga aaaaaacata
     3781 gtcctccgga gacgccaacc ccatcacccg ttctgccgcc gcatccgcat ggagataggc
     3841 cttctccaat tcaccgcccg gtttcacacg caggtcgccc ccaacgatct ccgtgtcttt
     3901 tttccaaaca ccgtgagacg cctcgtccac gctcctcaag tagaacacga tcaaacggaa
     3961 cgcctcctcc tcgtccaact ccagcaacag ctgaagcgcg gcggtttcct gcgtgcgcat
     4021 caaaacaacc tcatcccggg tcgccaatcc ccgaacattg ttttcaaaat gtcgcgcgag
     4081 ccgaggcagg atttccggcg gaaatcctcg ttccatcgcc ggcctgtcga aggacgtcca
     4141 ccgatcggtc aaaagaaccc cccgaatcgg gtccgccggg ctcgtcgcca gaacctgaaa
     4201 aacaccgaca tggtatccat gatcgctgag gatttcccaa acgcgtttca cccggcagtg
     4261 attgctggaa agactctctc cgttgatttc ccatgaggcc actccgtgga ttccaacggg
     4321 acagccgctg aaaacggtca tccatttcgt gggagaaagt ccccccccca tgttaatgga
     4381 accataccat ccctgttgaa aaagttttgt aagatgagga atgcttttcc ggcgcgaaag
     4441 gtcgcgaatc acgttttcgc tggccccgtc gacggccagc accacgacct tcggcgccgc
     4501 aaaaagcggc gagatgagga gaaacgccgt aatggtcaac gtaatttttt ttatcatgcc
     4561 gtattctagc aaatgaaaaa acggctccat cccctcttgc tggcgttttt gctgccggtg
     4621 gcgctgggcg cttctttcgt cggccgcccc tgtttcgtgg acgacagcta ccacctgctg
     4681 atggcgaaag gtctgctgga acaccctctc cgcccctacg atttcaaagc cgacgacgcc
     4741 ggcccgaaca accccggctg ggaatacggc cggcccccgc gcatggtgaa ccctccgctc
     4801 caccattatc ttctggcgct tttcctgaaa gccggcggag accggatctg gctggcgcgc
     4861 ctgggtttcc tccttctctc cggcctgagc gccctcctga ttttcctgct ctcccagcga
     4921 ttcatggtcc cccccctccc ggcgacggtc tttgccgtgt tgacgcccgc tttctggctt
     4981 tcgagttatt ccctgctgat cgacccgaca atgctcctgt ttttcctggc cgcgctgctg
     5041 ttctggatcg acggcctcag gaaaaattcc acgccgctgc ttgtcgccgc cgggttgtcg
     5101 atgggcgccg cctgcctgac gaaatacacc gccgccctcg tcgtccccct ggctttcctg
     5161 tggtggttgt tgcgcccaag agaagaacgt cgtttccgcc ccctcctctt cctcggcctt
     5221 cccttcctcc tcctcggcct ctggagcctc tggaacgtgc acacctacgg ggcgccgcac
     5281 ctcctcgcct cctccaaacg cgtggtgcag gagttccatt ggacgaatct gctcgttttc
     5341 ctcactttct tttccggcgt gttccttttt ccgctcttcg cctggctttg ggacctccgc
     5401 ctcgcccgtc gaccttttct cgccgccgcg gggctgacgc tgatcttgtt cgctctcctc
     5461 gccggtccct ggggcggctt ttcaatggat caagccctcc tggtgtccct tctggcggga
     5521 gggggcgttc tgtttttcca gctcgtcgcg caaaccgtcg tcgcccgggc gctcccctcg
     5581 gacgtcttcc tgctcctgtg gcttttcctg aattccatcc aaatgatctt catcatgggc
     5641 tggttcgccg gccggtacta cctcaccgtc ctgcctcccg cggtcttcct gttctatcga
     5701 gcgctccagc gccgctggcc ccacgcccca ggcgcgctgc ggcgtttctc gacgctcatc
     5761 gaggcgcttc tctttctgtc cgggttggcg ttggcgacgg cggatcatct tcaggccacg
     5821 gcgcggcggc gcatcgtcga cgaactggaa cgggacggct ggaccgacgc cggacgacgc
     5881 tgtttcgccc tcggagactc tttcacgggg gactatttaa aagccgccgg ctggaccacg
     5941 gcctttccgg aaacgcgatt tcaacacggg gacctcgtcc tcaaaaacga cgtggtgatg
     6001 ccggcgtggt ggttccgccc ggaaaaagac cgattccggc cgctcaaaat ctacgagtac
     6061 gcctcccgtt ttcctctccg cgtgatggac aaccaagggg cggccggttt ttacgcctcg
     6121 gtgtggggcg ccctcccgtt cagcttttcg cggagccccc tggaacgtta cgtcctcctg
     6181 gaatacgccg gggaggcccc gtcggagtca ccgcgccgcg cgttgcccca tcgggccgga
     6241 atttagtaga atggacacgc catggaaaac accccctccg gcaattccca caccatcctc
     6301 atcgccgacg acgaccgggt cgcccaacag accctggaaa ccctcctgac gggcatcggg
     6361 taccgggtcc tcgtggccca caacggggag gaggcccgcg accggatccg ggaattccgt
     6421 cccgacctct gcgtcctgga catcgac
//
LOCUS       DAOTFD010000144         6412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8647_length_6412_cov_4.782759, whole genome shotgun sequence.
ACCESSION   DAOTFD010000144 DAOTFD010000000
VERSION     DAOTFD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8647_length_6412_cov_4.782759"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="P5079_08305"
     CDS             <1..1158
                     /locus_tag="P5079_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRY30019.1"
                     /translation="LVKWAKMKGLALLGSGDCTHPQWMVELKKFLKPASGRGIYEYDD
                     VKFLLTGEVSCQYTRAGRTYRIHHIVFVPNFSTADRLNDSLARHGDLETEARPTVSLT
                     AEDFVKAVLDVSPECLVVPAHPWGLQYSLFSQQTGYEQLADAYGGQADNVRVMETGLS
                     CDPADVRRFAQADGRTLISNSDATHPFHLGREANIFDCPIDYKDIAAVLLKNDRARFL
                     GTVELFPEADRFYWKGHKACQKRAASRREADEKCPACGKPFAPAEADRMDDLADRTRE
                     QAAPQAEPFHRVVPLHEIIAEVLGFQPDAETVQKQYHQITTQMGPELEILLNWPEDRL
                     RKQLPLRVAESVLAVRRGEVTFEPGYDGVPGKARIKLPEGLTQETGQLKLF"
     gene            1174..1773
                     /locus_tag="P5079_08310"
     CDS             1174..1773
                     /locus_tag="P5079_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY30020.1"
                     /translation="MLVGAVILAAGHSERMGRPKALLEWEGDTFLGTIGRRLRRAGVS
                     EVSVVLGRDAETILKGWRRANERVLINRRPEDGQISSLKLALRELAVDPAPEAVLVCL
                     ADTPLVRVETYARLISAWAGSQGCLVVPRFNGKRGHPLVLDHRFWHETQLAPLEQGLH
                     WVTHRHPESLRDVDVDDGGVTADIDTPGDYQKALSPAGR"
     gene            1873..2352
                     /locus_tag="P5079_08315"
     CDS             1873..2352
                     /locus_tag="P5079_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30021.1"
                     /translation="MEKILLENKKDILTGMTFAIKELRGSYERRPIEEIYGHNEIYFK
                     AHAANLLSGDFQPLVDFADMITRKRALDRFRLHELLRASFSFKKVLYPLLVQQCWEDR
                     NELVKSIVALDRSVDRFVVNLSQAFVHYAKDYLARDPLEFPVWLENVWRRVSSEETN"
     gene            2402..3415
                     /locus_tag="P5079_08320"
     CDS             2402..3415
                     /locus_tag="P5079_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRY30022.1"
                     /translation="MKTMLRGKDWIETADWSVEELETVLDVAADLKRRFSLNEAHRIL
                     QDKTLFMMFFEQSTRTRNSTEAGMTQLGGHAHDLTPDKMQISHGETPKDTGMVLSRYG
                     HGIAIRNCFYKVGNKYIREVANYASVPVISLQCDVDHPCQTIADLMTIREKFGTNLRG
                     LKVAASWTYAPAYARPLSVTQGLITLLPRFGVDVTLAHPKEFYFMPHVMEKARAFAKQ
                     HGTKFEVVNEMDDSFKDAVVCYPKSWGCHQYLDDQKVPEAERKNVGMDLLGKYKSWVC
                     SEKRMKLAKKEAVYMHPLPADRGSEVEDAVIDGPQSIVFDQAENRLHTVKAIQALTMG
                     GRP"
     gene            3445..4467
                     /locus_tag="P5079_08325"
     CDS             3445..4467
                     /locus_tag="P5079_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30023.1"
                     /translation="MAEKKKSLLLAALAAVGALIVSAAVLYGLERSARTRIVDALIYR
                     DSELPALRFEPIPGARGELEGVPVHLNGRGLRDREIPVARPPAVKKRIVVLGDSITFA
                     RGVPPESAFPKKWEQFLGPSYEVINAGVCAYNGEQELAFFDVRLADLKPDLVVLALTP
                     DDFEDVPPTRFHFPRLKNFLRERSAFARSLMERSYRKRFARSTVPSGLAYAASPVRAP
                     KSGARKVAGEKPVDVRDAILARARGLLEKAGKLAETRQLRLVVLYVPRLDRINTDPAD
                     VERREALRRSSAELRRPFWDAFDALTAGLITENLLDLQNPYLSEKGHATLAQFIADRA
                     RREGIL"
     gene            4464..5405
                     /gene="arcC"
                     /locus_tag="P5079_08330"
     CDS             4464..5405
                     /gene="arcC"
                     /locus_tag="P5079_08330"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRY30024.1"
                     /translation="MSKTIVIAIGGNSLIRDAQHQTVKDQYDAVCETARHMVDLVEKG
                     HRLIITHGNGPQVGFILLRSEYSRGFLHEVPIDSIVADTQGAIGYQIQQAIENEMWRR
                     QVRRQVVTVVTQVLVNSDDPAFQKPTKPIGPFYKKEQAEERIKKDGWNMVEDAGRGWR
                     RVIASPKPVRIIESHAVKLLVKEGVIVISAGGGGIPVIRDKDGILRGTAAVIDKDYAS
                     ALLAKEIGADLFVISTGVEQVCLNFKKPDQKNLSRLTKEEAKRYLAEGHFAAGSMKPK
                     IEACLQFLESGGKEALITSPEKIKEALDGKTGTLIVA"
     gene            5429..>6412
                     /locus_tag="P5079_08335"
     CDS             5429..>6412
                     /locus_tag="P5079_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY30025.1"
                     /translation="MKSRILGFECLSCGEQADWGRIRYRCAKCGSNLNVVYDYAALKK
                     KMSPRRWSERPADLLRYRELLPLEKEATLPLSVGGTPLFQSGPLSEKIGHKALYLKDE
                     TRNPSASFKDRASSVVLLRAREERAAVVAAASTGNAGSSLACLAAAGGQASVIFIPKT
                     APKPKVAQLQVFGARVLAVNGNYDAAFDLCAEVCEKSGWYNRNTGLNPFTREGKKTVS
                     FEIFEQLGGRRPDWVLVPTGDGNIISGVWKGFRDLLSLGWIDRLPRLAAVQAEGSNAV
                     ARAWRTRQPEGPGKKWVYSVEKISATTRADSISVDLPRDAVGALRAVEESHG"
BASE COUNT         1254 a         1941 c         2123 g         1094 t
ORIGIN      
        1 ctcgtcaaat gggcgaagat gaaggggctg gccctcctgg gcagcggcga ctgtacccat
       61 ccccaatgga tggtggaact gaagaaattt ttgaaacccg cctccggacg gggcatctac
      121 gaatacgacg acgtgaaatt cctcctgacc ggggaggtgt cctgccagta cacccgcgcc
      181 ggaaggacct atcgcatcca ccacatcgtc ttcgtcccga atttttccac cgcggaccgg
      241 ctcaacgatt ccctggcccg gcacggcgac ctggagacgg aggcgcggcc caccgtcagt
      301 ctcacggcgg aggatttcgt gaaggcggtg ttggacgtct ctcccgaatg tctggtggtg
      361 ccggcccacc cgtgggggct gcagtattcg ctcttttctc agcagaccgg ttacgagcaa
      421 ctcgcggacg cctatggcgg ccaggcggac aacgtccgcg tgatggagac ggggctcagc
      481 tgcgatccgg cggatgtgcg gcgtttcgcc caggcggacg gccgcaccct catctccaac
      541 tccgacgcca cgcacccgtt ccacctcggc cgcgaggcga atatcttcga ctgccccatc
      601 gactataagg acatcgcggc cgttttgctg aagaacgacc gcgcgcggtt cctgggcacc
      661 gtggagctct ttccggaggc ggaccgtttc tattggaaag gtcacaaggc ctgccagaag
      721 cgcgccgcct cccggcgaga ggccgacgag aaatgtcccg cctgcggcaa accgttcgcc
      781 ccggccgagg cggaccgcat ggacgacctc gccgaccgga ccagggagca ggccgcgccc
      841 caggcggaac cgttccaccg ggtggtgccg ctgcacgaga tcatcgccga ggtgctgggg
      901 ttccagccgg atgcggaaac cgtgcagaaa caataccacc agatcaccac gcagatgggg
      961 ccggaactgg agatcctttt gaactggccc gaggaccggc tccgcaaaca gctgccgctg
     1021 cgcgtggcgg agagcgtctt ggccgtccgc cgcggcgagg tgacgttcga gccgggctac
     1081 gacggagtgc ccggcaaggc gcgcattaaa ttgcccgagg ggttgacgca ggaaacgggc
     1141 cagttgaagc ttttttgaaa gtttgaggcg gcgatgctcg tcggcgcggt gatcctggcg
     1201 gcgggccatt cggagcgcat gggccgcccc aaggcgctcc tggaatggga aggcgacacg
     1261 ttcctcggaa ccatcggccg gcgcctgcgc cgggccgggg tgtccgaagt gagcgtggtg
     1321 ttgggccgtg acgcggaaac gattctgaag ggctggcgcc gggccaacga acgcgtgctg
     1381 atcaaccgcc gtccggagga cggacagatt tcgtctttga aactggcgtt gcgggagctt
     1441 gccgtcgacc ccgcgccgga agccgtgctg gtgtgtctgg ccgacacgcc cctggtgcgc
     1501 gtggagacgt atgcgcggct gatctcggcc tgggccggtt ctcaaggttg cctggtggtg
     1561 ccgcggttca acggaaaaag ggggcacccc ctggtgttgg accaccggtt ttggcacgag
     1621 acgcagttgg cgccgctgga gcaggggctg cattgggtga cgcaccggca tccggaatcc
     1681 ctgcgggacg tggacgtgga cgacggcggc gtgacggcgg acatcgacac gcccggggac
     1741 tatcaaaagg cgttgtcgcc cgccgggcga tgacgcgggc cgggcggcgg aattttcgta
     1801 acgagaagga cggggcggcg ctgcggtaag cttgagaacg aggcgggggc gggaaccatg
     1861 ggaacagaaa gcatggagaa gattcttctg gagaacaaaa aggacatcct caccgggatg
     1921 acgttcgcca tcaaggagtt gcgcggaagc tacgaacgcc ggcccatcga ggagatctac
     1981 gggcacaacg agatctattt caaggcgcac gccgcgaacc tgttgagcgg ggattttcag
     2041 ccgctggtgg atttcgccga catgatcacg cgcaaacggg ccctggaccg gttccgcctg
     2101 cacgaactgc tccgcgccag cttcagtttt aagaaggtgc tctacccgct cctggtacag
     2161 caatgctggg aggatcggaa cgaactggtg aagtccatcg tggcgctgga ccggtccgtg
     2221 gaccgtttcg tcgtgaacct gtctcaggcg ttcgtccatt acgccaagga ttacctggcc
     2281 cgcgatccgc tggagttccc ggtgtggctg gagaacgtct ggcgccgggt gtcgtcggaa
     2341 gagacgaatt aggatttaaa gaagtcccat tgatcgaaac cttcaaggag gagacaagga
     2401 aatgaaaacg atgctgcgag gcaaggattg gattgaaacg gcggactgga gcgtggagga
     2461 gcttgagacg gttctggacg tggcggcgga tttgaaacgg cgcttttctt tgaacgaagc
     2521 ccaccggatc ctgcaggaca agaccctgtt catgatgttt ttcgaacagt cgacgcgcac
     2581 ccggaattcc accgaggcgg gcatgaccca gctgggcggc cacgcgcacg atttgacgcc
     2641 cgacaagatg cagatctccc acggcgaaac gcccaaggac acgggcatgg tgctctcccg
     2701 ctacggccac ggcatcgcca tccgcaactg cttttacaag gtcggcaaca agtatatccg
     2761 cgaggtcgcc aattacgcca gcgtgccggt gatcagcctt cagtgcgacg tggaccatcc
     2821 ctgccagacc atcgccgacc tcatgaccat ccgggaaaag ttcgggacca acctgcgcgg
     2881 cctgaaagtc gcggcctcct ggacgtacgc accggcctac gcgaggccct tgtccgtcac
     2941 ccaggggctc attacgctcc ttccccgctt tggcgtggac gtgaccttgg ctcaccccaa
     3001 ggagttttat ttcatgccgc acgtcatgga aaaggcccgg gctttcgcga aacagcacgg
     3061 caccaaattc gaagtcgtca acgagatgga cgattccttt aaagacgcgg tcgtctgcta
     3121 cccgaagtcc tggggttgcc accagtatct cgacgaccag aaggtgcccg aggcggaacg
     3181 gaagaacgtt gggatggacc ttctcggtaa atacaaaagt tgggtctgca gcgagaagcg
     3241 catgaagctc gccaaaaagg aagccgtcta catgcaccct ctgccggccg accgcggttc
     3301 cgaggtggaa gacgccgtga tcgacggtcc gcagtccatc gttttcgacc aggcggagaa
     3361 ccgtctccac acggtgaagg ccatccaggc cctcaccatg ggcggccgcc cgtaggaaga
     3421 ggaacgggaa caaccggcgg ccccatggcc gaaaaaaaga aatcgctgtt gttggccgcg
     3481 ctggcggcgg tgggggcgct gatcgtttcg gcggcggtgt tgtacggcct cgaacggtcg
     3541 gcccgaaccc gcatcgtcga cgcgctcatc taccgcgaca gcgagctccc ggccctgcgg
     3601 ttcgagccga tcccgggcgc tcggggagaa ttggaagggg ttccggtgca tttgaacggc
     3661 cgagggctgc gcgacaggga gatccctgtc gcgcgccctc cggccgtgaa aaaacgcatc
     3721 gtcgtgttgg gggattccat caccttcgcg cggggcgtcc cgccggagtc cgctttcccg
     3781 aagaaatggg agcagttcct gggcccttcc tatgaagtga tcaacgccgg cgtgtgcgcc
     3841 tacaacggcg aacaggaact ggcgtttttc gacgtccgtt tggcggactt gaaaccggac
     3901 ctggtcgtgc tggcgctgac gccggacgat ttcgaggatg tgccgcccac gcggttccat
     3961 tttccgcggc tcaagaattt cctgcgggaa cgttcggcct tcgcgcgttc cttgatggaa
     4021 cggtcctatc ggaaacggtt tgcccggtcg acggtgccgt ccggactggc gtacgcggcc
     4081 tctccggtcc gtgcgccgaa aagcggcgcc cgaaaggtgg ccggcgaaaa gccggtcgac
     4141 gtccgagacg ccatcctggc ccgcgcccgc ggtctcctgg aaaaagccgg aaaactggcc
     4201 gagacccggc aacttcgtct ggttgtcctc tatgtgccgc ggctcgaccg gatcaacacc
     4261 gacccggccg acgtcgagcg ccgggaggcc ctccgccgga gttcggcgga actccggcgg
     4321 ccgttctggg acgcctttga cgcgctcacc gccggcctca tcacggagaa ccttttggat
     4381 ctgcaaaacc cgtatttgag cgagaaaggg cacgcgacgt tggcacaatt catcgccgac
     4441 cgcgcccgga gggaagggat attatgagca agacaatcgt gatcgccatc gggggcaact
     4501 ccctgatccg cgacgcacaa catcagaccg ttaaggacca atacgacgct gtttgtgaga
     4561 cggcgcggca catggtggat ttggtggaga agggccaccg gctcatcatc acccacggca
     4621 acggacccca ggtgggattc atcctcctgc gttccgaata ttcccggggg ttcttgcacg
     4681 aggtcccgat cgattccatc gtggcggata cccagggggc catcggctac cagatccaac
     4741 aggccatcga gaacgagatg tggcggcggc aggtccgccg gcaggtggtc acggtggtca
     4801 cccaggtgct ggtgaactcc gacgacccgg ctttccagaa accgacgaaa cccatcgggc
     4861 ctttttataa aaaggaacag gcggaggaac ggatcaagaa ggacggttgg aacatggtgg
     4921 aagacgccgg ccgcggctgg cggcgcgtca tcgcctcgcc caaaccggtc cgcatcatcg
     4981 agagccacgc cgtcaagttg ttggtgaagg agggggtcat cgtgatctcg gccggcggcg
     5041 gcgggatccc cgtcattcgc gacaaggacg gcatcctccg cggaaccgcc gcggtgatcg
     5101 acaaggacta cgcctcggcc ctgttggcca aagagatcgg cgccgatctc ttcgtgatct
     5161 ccaccggcgt ggaacaggtg tgtctcaatt tcaaaaaacc ggatcagaaa aacctctccc
     5221 gcctcacaaa agaggaggcc aaacggtacc tggccgaggg gcatttcgcc gccggctcca
     5281 tgaaaccgaa gatcgaggcg tgtctgcagt tccttgagtc cggagggaaa gaggcgttga
     5341 tcacctcccc cgaaaagatc aaggaagcct tggacggcaa gacggggacg ctcatcgtcg
     5401 cctagggccg gcgccgggcg gagaacctat gaaatcgcgg atcctgggat tcgaatgtct
     5461 ttcctgcggc gaacaagccg actggggccg gatccgctat cgctgcgcca aatgcggttc
     5521 caacctcaac gtcgtctacg attacgcggc gttgaaaaag aagatgtctc cgcgccgctg
     5581 gagcgaacgt ccggcggacc tgctccgtta tcgggaactt ctgccgctgg aaaaagaggc
     5641 gacgttgccg ctgtccgtcg gaggaacgcc gttgtttcaa tcggggccgc tgagtgaaaa
     5701 gatcgggcac aaggcgctct acttgaaaga cgagacgcgg aacccgtccg cctccttcaa
     5761 agaccgcgcc agcagcgtgg tccttctgcg ggcccgggag gagcgggcgg cggtggtggc
     5821 cgccgcgtcc acggggaacg ccgggtcgtc cctggcctgc ctggcggcgg ccggcggcca
     5881 ggccagcgtc attttcatcc cgaagacggc gcccaaaccg aaggtggcgc agttgcaggt
     5941 gttcggcgcg cgtgtcctgg cggtcaacgg aaattacgac gcggccttcg acctctgtgc
     6001 cgaggtgtgt gagaagtccg gctggtacaa ccgcaacacc ggcctcaacc cgttcacgcg
     6061 cgaaggcaaa aagaccgtgt cgttcgagat cttcgaacag ctgggcggcc gccggccgga
     6121 ctgggtcctc gtgcccaccg gcgacggcaa catcatttcg ggcgtgtgga aagggttccg
     6181 ggaccttctg tcgttgggct ggatcgaccg gcttccgcgt ttggcggcgg tgcaggcgga
     6241 aggatcgaac gctgtggcgc gggcctggcg gacgcgtcaa ccggaggggc cggggaagaa
     6301 atgggtttac tccgtggaaa aaatatcggc caccacccgg gccgattcca tctcggtgga
     6361 tctgccgcgc gacgccgtcg gcgccctgcg ggccgtggag gagagccacg gg
//
LOCUS       DAOTFD010000145         6383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8699_length_6383_cov_4.722345, whole genome shotgun sequence.
ACCESSION   DAOTFD010000145 DAOTFD010000000
VERSION     DAOTFD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_6383_cov_4.722345"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5079_08340"
     CDS             <1..262
                     /locus_tag="P5079_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30026.1"
                     /translation="GPTYITYLLNEDANVTITLFDLLGYKVREWEFSAGSLGGRAGPN
                     TYSWDGANEGGAKVAAGGYILRIEVRGSKGTTTVIRKIGIIH"
     gene            346..1296
                     /locus_tag="P5079_08345"
     CDS             346..1296
                     /locus_tag="P5079_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRY30027.1"
                     /translation="MAAALVLLFLPACRTTGSKPAVREVRQGGFRYINPLLECENFSA
                     SGFRELRPFLRKIEAAVAGVLRSQRATHVSIYFRDLNNGPWFGIHEDEQFSPASLLKV
                     PILMTLLHQAETRPELLSLELPYVSADGDENVNQHHKPAEEVELGKSYPVDELLRRMI
                     VYSENNARPLLLKVLDTDEFLRLLDRLGIAVPMKEGVFPDDYMDIKDYASFFRILYNA
                     TYLRKENSEKALAILAQSDFREGLVADLPPDVLVAHKFGERRHNNLNQLHDCGIVYFP
                     GHPYLLGVMTRGKKFDELARVIAELSRLVYDEVNRQYGRP"
     gene            1448..2098
                     /locus_tag="P5079_08350"
     CDS             1448..2098
                     /locus_tag="P5079_08350"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRY30028.1"
                     /translation="MINRDKISDKTIGRLSQYRRFLNLLHENKVKNVFSYQIANSAGV
                     TSPQVRRDVMLLGYNGNPAKGYNVEALMECLAHYLDTPGGEGVVLVGFGNLGRALISY
                     FSKQRPNLSIVAVFDNDPALIGRVIHGVRCCSMEDFASVAQQNNVRVGILAVPADQAQ
                     LTADLMVAAGINGILNFALTTLQVPPHVYVERVDITTSVEKVVFFARKIRQERTLP"
     gene            2095..2568
                     /locus_tag="P5079_08355"
     CDS             2095..2568
                     /locus_tag="P5079_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY30029.1"
                     /translation="MNAESIGLLLDQYPGAGRDQLIPILQDIQERYGYLSKEAVVVIS
                     RRLNIPSSKIYGVATFYNQFRFLAPGKYHVQLCRGTACHVKGSAALLEEVKKELKVGP
                     GQTTPDGLFSLEVVACIGACGLSPVISVNGEFHAKVKTGGVKRIFDQYRAREKGN"
     gene            2570..4537
                     /locus_tag="P5079_08360"
     CDS             2570..4537
                     /locus_tag="P5079_08360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY30030.1"
                     /translation="MAADNPNDIAAPLAPDWVRDVLTGAALSPAAAENLALLRRHVLA
                     RPAVFVGTGTCGLGAGAQKTLDKAKAFFADRKLAADFIEVGCIGLCAMEPLLDVQLPG
                     KARLSFQEVTPQNAERILDEVLQGRVPREQLLGQHRPPKGSPLAVWEGVSFLDEHPFF
                     APQMRWVLANCGIIDPARINEYIAHGGYAAFAKTVGSLTPAELCDQVESSGLRGRGGG
                     GFPTGKKWKFALAQKADQKYLICNADEGDPGAFMDRAVIEGDPHRLLEGMAIAAYGIG
                     ATKAYVYIRAEYPLAIQRLKDAISQAKAYGFLGRNILDSGMDLDILIKMGAGAFVCGE
                     ETALMQSIEGKRGMPQPRPPYPAIKGLFGKPTVINNVETLANLPALMAMSPAKFASVG
                     TAKSKGTKVFALSGKVERTGLVEVAMGTPLRQIIFDIGGGITDGKKFKAVQIGGPSGG
                     CIPEQHLGIEIDYESLKTVGAMMGSGGLVVMDEGTCMVDLAKFFMDFIQRESCGKCIP
                     CREGTRRLLEILNRISEGRRRETGAEALERFKGVMYMNRLAEVIRDTSLCGLGQTAPN
                     PILSTLRWFRDEYEAHVYDRRCPAGVCAGLVHYEIDPVKCKGCTLCAKNCPSGAILGA
                     VKTPHEILMDKCIGCGSCRVLCKFDAVTVRS"
     gene            4553..6274
                     /locus_tag="P5079_08365"
     CDS             4553..6274
                     /locus_tag="P5079_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY30031.1"
                     /translation="MIEVNGQKIECKPEDTVLTALQRAGIFVPTLCYLPDLFANGACR
                     MCVVEVEGQRNLIPSCAFPVAENMKVSTHSPRVLRARKTIIELLCSNHPDDCLYCGRN
                     GSCELQRLTELLGVRERRYTGERSRHRPDVSNPSIARDPAKCILCGRCVRVCETTQSV
                     SAIDFIGRGSRTKIGTAFDAGLNVSSCINCGQCIKVCPTGALSEQSSLKEVHNALADP
                     HMTVVVQHAPSVSVTLAEEFGMKPGVDVNGVMVTALRRLGFDKVFDTSFSADLTIMEE
                     ASEFAHRLTRGGVLPMFTSCSPGWIKFVEQFYPKFLPNLSTCKSPQQMMGAVVKSYFA
                     KQAGLDPHKIFSVSVMPCTAKKFEAQRPEMLFNGLPDVDAVLTTRELAQMIRMRGLDL
                     NALEPDTADTPFGSRSTAGKIFGASGGVMEAALRTAHHLITGKELEALELKAIRGMDG
                     LKEASLRVGDVSVNVAVVSGLGNARKLMEEIASGARPNLHFVEVMTCPGGCVNGGGQP
                     YNASPEAVRARMQALYRIDREEKVRTSHGNKSVQRLYEEFLEKPCGKKSHQLLHTRYH
                     AREEVAL"
BASE COUNT         1191 a         2244 c         1907 g         1041 t
ORIGIN      
        1 cgggccgacc tacatcacct accttctgaa cgaggatgcc aacgtcacca tcaccctctt
       61 cgacctgttg ggatacaagg tgcgcgagtg ggagttctcc gcgggctcgc tcggcggccg
      121 cgccggcccc aacacctatt cctgggacgg cgccaacgag ggcggcgcca aagtggcggc
      181 cggcggatac atcctccgca tcgaggtgcg gggctccaag ggcaccacca ccgtcatccg
      241 gaagatcggg atcatccact gatccgaccc atcggccggc gggagggggg acgtttggcg
      301 catcgggagg gacgtttttt tcgaggacgg agaaaccggc gttggttggc ggccgcgctg
      361 gttcttcttt ttctgccggc ttgtcggacg accgggtcca agcccgccgt ccgagaggtg
      421 cgccagggcg ggttccgcta catcaacccg ctgttggagt gcgagaattt ttcggcgagc
      481 ggctttcgcg agctccggcc gttcctgcgg aagatcgagg cggcggtggc cggcgtcctc
      541 cgaagccaac gcgccaccca cgtttccatc tacttccgcg acctcaacaa cggcccgtgg
      601 ttcggcatcc atgaggacga acagttctcg cccgccagcc tgctcaaggt ccccatcctg
      661 atgaccctcc tgcaccaggc ggagacccgc cccgagctcc tttccctgga actgccctac
      721 gtctccgccg acggcgacga gaacgtcaac cagcaccaca agccggcgga ggaggtggaa
      781 ctcggaaaga gctatcccgt cgacgaactc ctgcggcgga tgatcgtcta ctccgagaac
      841 aacgcccgcc cgctgctgtt gaaggtgctg gacaccgacg agttcctccg gcttttggac
      901 cgcctcggca tcgcggtccc catgaaggag ggggtgttcc cggacgacta catggacatc
      961 aaggactacg cgtctttctt ccgcatcctg tacaacgcca cctacctgcg caaggagaat
     1021 tcggaaaaag ccctggcgat cctcgcccag agcgatttcc gggagggtct ggtggcggac
     1081 ctgccgccgg acgtcctggt ggcgcacaag ttcggcgaac gccgccacaa caacctcaac
     1141 cagctgcacg actgcggcat cgtgtatttt cccgggcacc cctacctgct cggggtgatg
     1201 acgcggggga aaaaattcga tgaactggcg cgggtcatcg ccgagctttc ccggttggtg
     1261 tacgacgaag tgaaccgtca atacggccgc ccctgacccg cccgtccggc ggtcgatcac
     1321 ggaccgccct cctccgcctg ttcgaaatat tccgccgaag ctccctcatt gaaaaatagt
     1381 gcttgacaag cctctcgagg tttattaatg ttattgtgaa aaaaataaca gcgttaaaaa
     1441 gataacgatg attaatcgcg ataagatctc tgacaagacc atcggccgac tcagtcagta
     1501 ccggcgattt ttgaacctgc ttcacgagaa taaagtcaag aacgtctttt cttatcaaat
     1561 cgccaactcg gccggagtca cctcccccca ggtccgccgc gacgtcatgc ttctgggtta
     1621 caacggcaac ccggccaagg gatataacgt ggaagcgttg atggagtgcc tcgcccatta
     1681 tctggacacc ccgggcggcg agggggtcgt gttggtgggg ttcggcaacc tggggcgggc
     1741 gctcatctcc tatttttcga aacagcggcc caacctgtcc atcgtggcgg tgttcgacaa
     1801 cgatcccgcc ctcatcggcc gggtcatcca cggcgtgcgc tgttgttcca tggaggattt
     1861 cgcgtccgtc gcgcagcaga acaacgtccg cgtgggcatc ctggccgtcc ccgcggacca
     1921 ggcccagctc accgccgacc tcatggtggc cgccggcatc aacggcatcc tcaatttcgc
     1981 cttgaccacg ctccaggtcc ccccgcacgt ctatgtggaa cgggtggaca tcaccacctc
     2041 cgtcgagaaa gtcgtttttt tcgcccggaa gatccggcag gaaaggacac tcccatgaac
     2101 gcggaatcca tcggcctcct cctcgatcaa tatcccggcg ccggccgcga ccagctcatc
     2161 cccattctgc aggacatcca ggaacgctac ggctacctct ccaaggaggc ggtcgtcgtc
     2221 atcagccgcc ggctgaacat cccgtccagc aagatctacg gcgtggccac cttctacaac
     2281 cagttccggt tcctggcgcc gggaaaatac cacgtccaac tctgccgcgg caccgcctgc
     2341 cacgtcaagg gctccgccgc cctcctcgaa gaggtgaaga aggagctcaa ggtggggccg
     2401 ggccagacca cccccgacgg cctcttcagc ctcgaggtgg tggcctgcat cggggcgtgc
     2461 ggactgtccc ccgtgatttc cgtcaacggc gagttccacg ccaaggtcaa gacgggcggc
     2521 gtcaaaagga tattcgacca ataccgcgcc cgggaaaagg ggaactgaca tggccgccga
     2581 taacccgaac gacatcgccg cccccctcgc cccggactgg gtccgcgacg ttttgacggg
     2641 cgccgccctc tcccccgcgg cggccgagaa cctcgcgctt ctccgccggc acgtcctcgc
     2701 gcggccggcg gtgttcgtcg gcaccgggac gtgcggcctg ggcgccggcg cccagaagac
     2761 gttggacaag gccaaagctt tttttgcgga ccggaagctc gccgccgatt tcatcgaggt
     2821 gggctgcatc ggcctctgcg ccatggaacc gctcctcgac gtgcagctcc ccggcaaggc
     2881 ccggctcagc tttcaggagg tcacgcccca gaacgcggaa cggatcctcg acgaggtcct
     2941 tcaggggcgc gtcccccgcg aacaacttct cggccagcac cggccgccga agggttctcc
     3001 gctcgcggtg tgggagggcg tgtccttcct cgacgaacat ccctttttcg cgccgcagat
     3061 gcggtgggtc ctggccaact gcggcatcat cgaccccgcc cgcatcaacg aatacatcgc
     3121 ccacggcgga tacgccgcct tcgccaagac cgtcggttcc ctcaccccgg cggaactctg
     3181 cgaccaggtg gaatcctcgg ggctccgggg ccgcggcggc ggcgggttcc ccaccggcaa
     3241 gaaatggaag ttcgccctgg cccagaaggc cgaccagaaa tacctcatct gcaacgccga
     3301 cgaaggcgac cccggcgcgt tcatggaccg cgccgtcatc gagggcgacc cgcaccggct
     3361 cctcgagggc atggccatcg ccgcctacgg catcggcgcc accaaggcct acgtgtacat
     3421 ccgggcggaa taccccctgg ccatccagcg gctcaaggac gccatctccc aggccaaggc
     3481 ctacgggttc ctgggacgga acatcctgga cagcggcatg gatctggaca tcctcatcaa
     3541 aatgggcgcc ggcgccttcg tctgcggcga ggagaccgcc ctcatgcaga gcatcgaagg
     3601 caaacgcggc atgcctcagc cccggccgcc ctaccccgcc atcaaagggc tcttcggcaa
     3661 acccaccgtc atcaacaacg tggagacgct cgccaacctg cccgcgctga tggccatgtc
     3721 tccggccaag ttcgcgtccg tgggcaccgc caaatccaag ggcaccaagg tgttcgccct
     3781 ctccggcaag gtggaacgca ccgggctggt ggaggtggcc atgggcaccc cgctccggca
     3841 gatcatcttc gacatcggcg gcggcatcac cgacggaaag aagttcaagg cggtgcagat
     3901 cggcggcccc tccggcggat gcatccccga acagcacctg ggcatcgaga tcgactacga
     3961 atccctcaag accgtgggcg ccatgatggg gtccggcggg ctggtggtca tggacgaggg
     4021 cacctgcatg gtggacctgg ccaagttctt catggatttc attcagcggg agagctgcgg
     4081 caagtgcatc ccctgccgcg aaggcactcg gcggctgctg gagattttga accgcatctc
     4141 cgagggccgc cgccgcgaga cgggcgccga ggcgctggag cgtttcaagg gcgtgatgta
     4201 catgaaccgc ctcgcggagg tcatccgcga caccagcctc tgcggcctgg gccagaccgc
     4261 ccccaacccc atcctcagca ccctgcgctg gttccgcgac gagtacgagg cccacgtcta
     4321 cgaccgccgc tgccccgcgg gcgtctgcgc ggggctggtc cactacgaaa tcgacccggt
     4381 caagtgcaag ggctgcaccc tctgcgccaa gaactgtccc agcggcgcca tcctcggcgc
     4441 cgtgaagacg ccgcacgaga tattgatgga caaatgcatc ggctgcgggt cctgccgcgt
     4501 cctctgcaag ttcgacgccg tcaccgtccg ttcctaagga gatttcgcca tgatgatcga
     4561 agtcaacgga caaaaaatcg agtgcaaacc ggaggacacc gtcctcacgg cgctccagcg
     4621 cgcgggcatc ttcgtgccca cgctctgtta cctgcccgac ctcttcgcca acggcgcctg
     4681 ccgcatgtgc gtggtggagg tggaaggcca gcgcaacctc atccccagct gcgccttccc
     4741 cgtggcggag aacatgaagg tctccaccca ctcgccgcgc gtgctccgcg cccgcaagac
     4801 catcatcgag ctcctctgca gcaaccatcc ggacgactgc ctctactgcg gccgcaacgg
     4861 cagctgcgag ctgcagcggc tcaccgagct cctgggcgtc cgcgaacggc gctacaccgg
     4921 cgaacgcagc cgccaccggc ccgacgtctc caacccgtcc atcgcccgcg acccggccaa
     4981 gtgcatcctc tgcggccggt gcgtgcgcgt ctgcgaaacg acgcagagcg tctcggccat
     5041 cgatttcatc ggccggggca gccgcaccaa gatcggcacc gctttcgacg ccgggttgaa
     5101 cgtctccagc tgcatcaact gcggccagtg catcaaggtg tgccccaccg gggcgctgtc
     5161 ggaacagagc tccctcaagg aggtacacaa cgccctggcg gacccccaca tgaccgtcgt
     5221 ggtccagcac gccccgtcgg tgtcggtgac cctggcggag gagttcggca tgaaaccggg
     5281 cgtggacgtc aacggcgtca tggtgaccgc cctccggcgg ctggggttcg acaaggtgtt
     5341 cgacacctcc ttctccgccg acctcaccat catggaggag gccagcgagt tcgcgcaccg
     5401 gctcacccgc ggcggcgtcc tgcccatgtt caccagctgc tccccggggt ggatcaagtt
     5461 cgtcgaacag ttctatccga agttcctgcc caacctctcc acctgcaaga gcccccagca
     5521 gatgatgggc gcggtggtga agagctattt cgccaaacag gccggactgg acccgcacaa
     5581 gatcttcagc gtctccgtca tgccctgcac cgccaagaag ttcgaggccc agcggcccga
     5641 gatgctcttc aacggcctgc ccgacgtgga cgccgtcctc accacgcgcg aactggccca
     5701 gatgatccgc atgcgggggc tggacctgaa cgccctggaa cccgacaccg ccgacacccc
     5761 cttcgggtcc cgcagcaccg cggggaagat cttcggcgcc tccggcggcg tcatggaggc
     5821 cgctctccgc accgcccacc acctcatcac cggaaaggaa ctggaggccc tcgagctcaa
     5881 ggccatccgc ggcatggacg gcctcaagga ggcctccctc cgggtcggcg acgtctccgt
     5941 gaacgtggcg gtggtcagcg gactgggcaa cgcccgcaag ctgatggagg agatcgcctc
     6001 cggcgcgcgc cccaacctgc atttcgtcga agtgatgacc tgccccggcg gctgcgtcaa
     6061 cggcggcggc cagccctaca acgcctcccc cgaggccgtt cgcgcgcgca tgcaggccct
     6121 ctaccgcatc gaccgcgagg agaaagtccg cacctcccac ggcaacaaga gcgtccagcg
     6181 gctctacgag gaattcctgg agaaaccttg cggcaagaag agccaccagc tcctccacac
     6241 ccgctaccac gcgcgggaag aagtggcgct gtaaaaagga gagaccatga tcgaaaacgc
     6301 ccccaccgtc ctcctcgtcg acggcgacgt cgagttccag aactggctgg agacccgctt
     6361 caccgaaaag gggttcgccg tcc
//
LOCUS       DAOTFD010000146         6379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8706_length_6379_cov_4.716003, whole genome shotgun sequence.
ACCESSION   DAOTFD010000146 DAOTFD010000000
VERSION     DAOTFD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_6379_cov_4.716003"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6379
                     /locus_tag="P5079_08370"
     CDS             <1..>6379
                     /locus_tag="P5079_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30032.1"
                     /translation="YAPTRQLMNGTTTFDIYFRTAKSSHNVTASGSYTAFIADKVGVK
                     YTSPAALQMVFPGETAVPGSGTGLHGLPDLNGDNSDGVESFIAGTPIPVEVRLVDQFF
                     NVVSAGVMPQVEVVNDDSNSNSLEESPASLTGGTRTFNVTIRTANSVLDGVADHWGVT
                     VQEQPVTFGYTSSTRAITLQSDTAQNLLIFAPGQTFAPGSTTGKGGVIGSLTAGTAYN
                     LTVRAVDQYNNRDLDATHNVTLDTTDPWDNENIPAPFTSIGLFQGGGVFPNWRPLTAM
                     SPMDITVSTAAGGYLTPQTVSIPQVKADTTTVKLQLLIPGETAVPGSSTGKATVLGSF
                     TAGSEFTVTVRTVDNNWNVVTTTHPWVKINTFWNSDFSPSDANYNWDGSALYSANYGV
                     GLTSVPIGGSGEATFGVTLKYAGGANSHHLTAGADALPGVTSDSPNFTVVPAALSGLQ
                     IVVPGETVAPGKESGQVGKREASLPTAWTAGSPANVTVNAVDPYWNKVSASSTVYLMT
                     GYDLVLNPAGDPNLTAKVSTQTLVGGTTVFPAVMVTRASGKTIAVMDQLPSAEPSGIA
                     NYTQGVTGNSLPFSVNANVSKRLHVVLPGQTLAEGKPPYGGAGGRNSSPSDIAAGQNY
                     SVTVYLTDFYYNRKTTSSVAGQTTLRVTTTDPNDGNDPDVTQSIPIGSDNTNFMHVFQ
                     TAGNWTLTAQDVTGDGSSHSDSVSSAFNVIAATYPAGTQKFLLVMPGETHLPGTVVGK
                     TGAMEDFIAGMPRNVTILGVDQYSNIIQDNPGVQLNTNDGFAAFGTLTPPVSLGSATT
                     NVTFKAARVTSTQLGATQNGSGTTYPGVNYTAVSISTITVKPATANRLLVLAPGETAV
                     PGSSYGRQGYPDADYNNSADGDPLVDPGLIDKFIAGTQYNFNVKATDDYYNLVSTASA
                     SIKFETDDPFDALPAEQNLTGGATIFAHTFVSASPSGWTVTVSTGAGSTNLYTSTTSA
                     KIVVRGAPSSKLQVLLPGETAVPGSSTGKAGSPDANGDNADGIQSLVAGTTIQVTVKG
                     VDTNFNVDAAALASVHMITADPWDDEPPANLPLVNGTTVFSVLFVTTGAHTVTVSDQD
                     GTALDSTSSAAMPVVAGAANRLLVVLPGETHAPGRPPYEAGGMDALPTANPVGGRVGT
                     AANYNWTAGISTTVRVMATDKYWNLASDSRQVKLTPGDNSPLISIATQSLVGGSTAFS
                     VALYTAHVATGTAYDFLAFDDSLVKFTPAVYRTTNVLVLPDTGNTRMRVLADDLQRAA
                     GTTSGVSGSVSDKTAGIPFSVTVDVTDQYGNMVNFAPTVRVTAADDAFAPNLPRNVPL
                     TGGTSSFMVTLVTERTDDSGPPNEATTPSSSRLQVYNITYGSVTVNVEVDDDQSKRRL
                     LLLLPGETAVPGSLAAGGKIGTPSTFSAGQNINVTIRAVDDYFNRVENNNPNVTLTSN
                     DPFAAMPLINGQLMSNGQYTQDIFLQTANTAPGWVLTSTSTDPEYHVPDTASPVPIQA
                     ANLEKLQVILPGETAVPGSTTGKVGTRSVQRANEPFYVQVRAVDPFHNVISTINTDTV
                     RVTTKDPFDADPGVFSLSGGQVAAPGVPVTLFVATDTIAGGFQEIYARGESLNIGTST
                     CTALAVNPSTNTNQIQLQVLVPGETAVPGSSAGLPSAGQIGSAASKDVGDTFTVMVRF
                     TDSKFNKIRQGMGLALPQIVASSDDPFDLEPIAANLDASGTRTFNFSFQKENLAPGWR
                     IYVSTYSGSPITVPSDLSSYIVTYSTTADRLQLLLPGETAVPGSGPNATYPDARGRAG
                     VPDKDGDNGLDGDAETGNIDRFVAGVPATVTVRVTDRFYNLKPLAAPLVKIQTSDPFD
                     AHPGNKTLTGGATTFVVTFLTNTLEPNATSWWVMASTASGVNYATDTVTGVSVTAAPP
                     TRLQVLVPGETAVPGSSTGKTGEPDYVPGGAKDYFVAGMIFPVTVRLVDDNFNREVAL
                     GNYSATPKLSADDDYDTIDLASYTVLGAGLYQFAYATLNTRNEPGDNMPDVPRDLGWR
                     ITATDIQAIPYAAGVSTYVPVRAGDAAKLMVYVRGEQHVEGNLAGGGRSGSPDAEMAG
                     STFPVTALVTDVAFNRVTSDNGTSITLTCDDAFATLPGAQTVVQGSTTFNVW"
BASE COUNT         1156 a         2361 c         1909 g          953 t
ORIGIN      
        1 tacgcgccca cgcggcagtt gatgaacggc accaccacct tcgacatcta tttccgcacc
       61 gccaagtcgt cccacaacgt caccgccagc ggcagctaca ccgccttcat cgcggataag
      121 gtgggggtga aatacacctc gcccgcggcg ttgcagatgg tcttccccgg cgagaccgcg
      181 gtgcccggtt ccggcaccgg ccttcacggt ctaccggacc tgaacggcga caactccgac
      241 ggtgtggaga gtttcatcgc cggcacgccc atccccgtgg aggtgcggct ggtggaccag
      301 ttcttcaacg tggtgagcgc cggggtcatg ccgcaggtgg aggtggtcaa cgacgacagc
      361 aactccaatt cgctggaaga gtctcccgcc agtctcaccg gcggcacccg caccttcaac
      421 gtcaccatcc gcaccgccaa cagtgtgctg gacggagtgg ccgaccattg gggcgtcacc
      481 gtgcaggaac aacccgtcac gttcggatac acctcctcca cccgcgccat caccctgcag
      541 tccgacacgg cccagaacct gttgatcttc gcgccggggc agaccttcgc gccgggttcc
      601 accaccggca agggcggcgt catcggttcc ctcaccgcgg gcaccgccta caacctcacg
      661 gtgcgggccg tggaccaata caacaaccgc gacctggacg ccacccataa cgtgaccctg
      721 gacaccaccg acccgtggga caacgagaac atccccgcgc ccttcacctc catcgggctt
      781 ttccagggcg gcggcgtgtt ccccaactgg cggccgctca ccgccatgtc gcccatggac
      841 atcaccgtgt ccaccgccgc gggcgggtat ctcacgccgc agactgtcag tattccgcag
      901 gtgaaggccg acaccaccac cgtgaagctc cagctcctga tccccggcga gaccgcggtg
      961 cccggatcct ccaccgggaa ggccaccgtg ctggggtcct tcaccgccgg gtccgaattt
     1021 accgtcacgg tgcgcaccgt ggacaacaac tggaacgtcg tcaccaccac gcacccgtgg
     1081 gtgaagatta acacgttctg gaactcggat ttcagcccgt cggacgccaa ctacaactgg
     1141 gacggttccg cgctctattc ggccaactac ggcgtggggc tcaccagcgt gcccatcggc
     1201 ggcagcgggg aggccacctt cggcgtcacc ctcaaatacg ccggcggcgc caacagccac
     1261 cacctcacgg ccggggcgga cgcgttgccc ggcgtcacct ccgactcgcc gaacttcacg
     1321 gtggtgcccg ccgcgctcag cggcctgcag atcgtggtgc ccggcgagac cgtggcgccc
     1381 ggaaaagaat ccggccaggt cggcaaacgg gaagccagct tgcccacggc ctggaccgcc
     1441 ggatccccgg ccaacgtcac cgtcaacgct gtggacccgt actggaacaa ggtctcggcc
     1501 agcagcacgg tgtacctgat gacggggtat gacctggtgc tcaaccccgc cggcgacccc
     1561 aacctcaccg ccaaggtctc cacccagacg ctggtggggg ggaccacggt gttcccggcg
     1621 gtgatggtga cgcgcgcttc cggtaaaacc atcgcggtca tggaccagct tccctccgcg
     1681 gaacccagcg gcatcgccaa ctacacccag ggcgtcacgg ggaacagttt gccgttcagc
     1741 gtgaacgcca acgtgtccaa gcggctgcac gtggtgctgc ccggccagac gctggccgaa
     1801 ggaaagccgc cctacggcgg cgccggcggc cgcaacagct ccccgtcgga catcgccgcc
     1861 ggacagaact actccgttac cgtgtacctc acggatttct attacaaccg caagaccacc
     1921 agctcggtgg ccggtcagac caccctgcgc gtgaccacca ccgatcccaa cgacgggaac
     1981 gatccggacg tcacccagtc catccccatc gggtcggaca acaccaattt catgcacgtg
     2041 ttccaaacgg ccggcaactg gaccctcacc gcgcaggacg tgacgggcga cggctcgtcg
     2101 cattccgaca gcgtgtcgtc ggcgttcaac gtgatcgcgg ccacctatcc ggcgggcacg
     2161 cagaagttct tgctcgtcat gcccggcgag acccacctgc ccggcaccgt cgtcgggaag
     2221 accggcgcca tggaggactt catcgccggc atgccccgca acgtgacgat cctcggtgtg
     2281 gaccagtatt cgaatatcat tcaagataac ccgggggtgc agttgaatac caacgacggg
     2341 ttcgccgcct tcggcacgct cacaccgccg gtgtccctgg gttcggccac caccaacgtc
     2401 accttcaagg cggcccgggt cacctccaca cagctgggcg ccacgcagaa cgggtccggg
     2461 accacctatc ccggcgtcaa ctacaccgcg gtgagcatca gcaccatcac ggtgaaaccc
     2521 gccaccgcca accggctgtt ggtgctggcg ccgggcgaga ccgcggtgcc gggctcctcc
     2581 tacggccgac aagggtatcc ggacgccgac tacaacaaca gcgccgacgg cgatcccctg
     2641 gtggaccccg gcttgattga caagttcatc gccggcaccc agtataattt caacgtcaaa
     2701 gccacggacg attactacaa cctggtgtcc accgcttcgg cttccatcaa gttcgagacg
     2761 gacgacccgt tcgacgccct cccggcggaa cagaatctca ccggtggcgc caccatcttc
     2821 gcccacactt tcgtgagcgc cagcccgtcg ggatggaccg tcacggtctc caccggcgcg
     2881 gggtccacca acctgtatac ctccaccacc tcggcgaaga tcgtggtgcg gggcgccccg
     2941 tcctccaagc tgcaggtgtt gctgcccggg gagaccgcgg tgccgggttc cagcaccggc
     3001 aaggccggct cgccggacgc caacggggac aacgcggacg gcatccagtc cctggtggcc
     3061 ggcaccacca tccaggtgac ggtgaaaggc gtggacacca atttcaacgt ggacgccgcc
     3121 gccctggcgt cggtgcatat gatcaccgcc gacccgtggg acgacgagcc gccggccaac
     3181 ctgccgctcg tcaacggcac caccgtgttc tccgtgctgt tcgtgaccac cggggcccac
     3241 accgtcaccg tgtccgacca ggacggcacc gcgctggaca gcacctcctc ggccgccatg
     3301 ccggtggtgg cgggtgcggc caaccggctg ttggtggtcc tgcccggcga aacccacgcc
     3361 cccggccggc cgccctacga ggccggcggt atggatgctc ttccgaccgc caaccccgtt
     3421 ggcggacgcg tggggaccgc cgccaactac aattggaccg ccggcatctc caccaccgtg
     3481 cgggtgatgg ccacggacaa atattggaac ctggcctccg acagccgcca ggtgaaactg
     3541 acccccggcg acaacagccc gctcatctcc atcgccaccc agtcgctggt gggggggagc
     3601 accgcgttct cggtggcgtt gtacaccgct cacgtggcca cgggcacggc gtatgatttc
     3661 ctggcgttcg acgattcgtt ggtgaagttc accccggcgg tgtaccgcac caccaacgtg
     3721 ctggtgctgc ccgacaccgg caacacccgc atgcgggtgt tggccgacga cctccagcgc
     3781 gccgccggca ccaccagcgg cgtgagcgga agcgtgtcgg acaagaccgc cggcatcccc
     3841 ttctcggtca cggtggacgt cacggaccag tacggcaaca tggtgaattt cgcgcccacg
     3901 gtgcgcgtca ccgccgccga cgacgcgttt gcgcccaacc tgccgcggaa cgttccgctg
     3961 accggcggca ccagttcctt catggtgacg ctggtgacgg aacgcaccga cgacagcggc
     4021 ccgcccaacg aggccaccac gccttccagc agccgtctgc aggtgtacaa catcacctac
     4081 ggctcggtca ccgtcaacgt cgaggtggac gacgaccagt ccaaacggcg gcttctgctg
     4141 ttgttgccgg gcgagaccgc ggtgcccggt tctctggccg cgggcggaaa gatcggcacg
     4201 cccagcacct tctccgccgg ccagaacatc aacgtcacca tccgcgccgt ggacgattac
     4261 ttcaaccgcg tggagaacaa caaccccaac gtgacgttga cctccaacga cccgttcgcg
     4321 gccatgccgc tcatcaacgg ccagctcatg agcaacggcc agtacactca ggacatcttc
     4381 ctgcaaaccg ccaacaccgc ccccggctgg gtcctcacct ccaccagcac ggatcccgaa
     4441 tatcacgttc cggacaccgc cagccccgtg cccatccagg cggccaacct ggaaaaactg
     4501 caggtcattc tgccgggcga gaccgcggtg ccggggtcca ccaccggcaa ggtgggaacg
     4561 cggtcggtgc agcgggccaa cgagccgttc tacgtgcagg tgcgcgcggt ggacccgttc
     4621 cataacgtga tctccaccat caacaccgac accgtccgcg tgaccaccaa ggacccgttc
     4681 gacgccgacc ccggcgtgtt ctctctcagc ggcggccagg tggcggcgcc gggggtgccg
     4741 gtgacgctct ttgtggccac cgacaccatc gccggcgggt tccaggagat ctacgcccgc
     4801 ggcgaaagct tgaacatcgg caccagcacc tgcaccgccc tggccgtgaa ccccagcacc
     4861 aacaccaacc agatccaact gcaggtgttg gtgcccggcg agaccgcggt gcccggctct
     4921 tccgcgggac tgccctccgc cgggcagatc ggttccgccg cgtccaagga cgtgggcgac
     4981 accttcaccg tgatggtgcg gttcacggat tccaagttca acaagatccg gcagggcatg
     5041 ggcttggccc tgccgcagat cgtcgcctcc tccgacgacc cgttcgatct ggagcccatc
     5101 gccgccaacc tggacgccag cggcacccgc accttcaatt tcagtttcca gaaggagaac
     5161 ctggcgcccg gctggcgcat ctacgtttcc acttactccg gttcgcccat cacggtgccg
     5221 tcggacctgt cgagctacat cgtgacctac tccaccaccg ccgaccgtct gcagttgttg
     5281 cttccgggcg agaccgcggt gccggggtcc ggccccaacg ccacctatcc cgacgcccgc
     5341 ggccgggccg gcgtgccgga caaggacggc gataacggcc tcgacggcga cgccgaaacg
     5401 ggcaacatcg accggttcgt ggccggggtg ccggccaccg tgaccgtgcg cgtcaccgac
     5461 cggttctaca acctcaagcc cttggccgcg ccgctggtga agatccagac cagcgacccg
     5521 ttcgacgccc atcccggcaa caaaacgctg accggcggcg ccaccacctt cgtggtgacg
     5581 ttcctcacca acacgctgga acccaacgcc acctcgtggt gggtgatggc gtccaccgcc
     5641 agcggcgtca attacgccac ggacaccgtc accggagtga gcgtgacggc cgccccgccc
     5701 acgcggttgc aggtgttggt gccgggcgag accgcggtgc ccggttcctc caccggcaag
     5761 accggcgagc cggattacgt gcccggcggc gccaaggact atttcgtggc ggggatgatc
     5821 ttcccggtga cggtgcggct ggtggacgac aatttcaacc gcgaggtggc gctgggcaac
     5881 tacagcgcca cgccgaaact ctcggccgac gacgattacg acaccatcga cctggcctcc
     5941 tacaccgtgc tgggggccgg cctctaccag ttcgcctacg ccacgttgaa cacccgcaac
     6001 gagccgggcg acaacatgcc cgacgtgccg cgggacctcg gctggcgcat caccgccacc
     6061 gacatccagg ccatccccta cgccgccggc gtgtccacct acgtgccggt gcgggcgggc
     6121 gacgccgcca aactgatggt gtatgtgcgc ggcgaacaac acgtggaagg caacctcgcc
     6181 ggcggcggcc ggagcggctc gccggacgcc gagatggccg ggtccacgtt cccggtgacc
     6241 gcgctggtca ccgacgtggc gttcaaccgg gtcaccagcg acaacggcac ctccatcacc
     6301 ctcacctgcg acgacgcttt cgccaccctg ccgggggcgc agacggtggt tcagggaagc
     6361 accaccttca acgtgtggc
//
LOCUS       DAOTFD010000147         6377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8709_length_6377_cov_4.198671, whole genome shotgun sequence.
ACCESSION   DAOTFD010000147 DAOTFD010000000
VERSION     DAOTFD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8709_length_6377_cov_4.198671"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="P5079_08375"
     CDS             complement(<1..196)
                     /locus_tag="P5079_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30033.1"
                     /translation="MGSDRNSAFSEKTRSVLVVRLSSLGDIILAAPVFENIKAAFPSA
                     RLSVLVKKNYAEVLRGMPAVD"
     gene            complement(201..1487)
                     /locus_tag="P5079_08380"
     CDS             complement(201..1487)
                     /locus_tag="P5079_08380"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRY30034.1"
                     /translation="MLILYSVLILLFWPLVVLGMVWRYGLRRTLRGLPERFLPPRRPS
                     VHPSPLVWVHAASVGEVRAVEPWLRGFFSRFETLRVGLTTTTVAGKELAVKLFPTAEV
                     RLAPVDLAACVRGLLEAWRPAALVLVETELWPNWIQTAADLNVPLLLINGRISDRSLK
                     SYLLLRWFWGPLLSRFAVVGAQSPVHAERFARIGTEREKIVVTGNLKYDLPLRDAASR
                     PALFRKYGLQENDRVWVCGSLHPPETEFVLQVRNGLLKEGVEVKLVIAPRHLESVPGI
                     VRSLEKENVPYSLRSQFSASAAPEMSTPVMVLDTIGELPEVYGLASWVFVGGSLVPRG
                     GQNLLEPARWGAPVLFGPHMENFREMAAMFLEQRAAVRVADGDGLLSEMRALAADRDR
                     SRRLGEAARRAADSQRGAVERNLDLLAKILPLRSEG"
     gene            complement(1481..2158)
                     /locus_tag="P5079_08385"
     CDS             complement(1481..2158)
                     /locus_tag="P5079_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY30035.1"
                     /translation="MLPRWWDSVLMWGVSWLGWGVLQFIGRTSRIERHFEPRAEELFR
                     SGRPLIYALWHRFQLLSIYIHRNEDILVLVSRSRDGELVARPLRRMGFRTARGSSSRG
                     GGAALLEVLGDLESGRRVVFTPDGPRGPLGAVQPGVVAAAQKTGVPVLPVAWAGRRVK
                     ALSSWDRFMIPYPFGRYQVVYHAPVDIAPEEAGGEEKIRRALDAAARLAEELLSEKTR
                     PAGKTIC"
     gene            complement(2203..2811)
                     /locus_tag="P5079_08390"
     CDS             complement(2203..2811)
                     /locus_tag="P5079_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30036.1"
                     /translation="MKKIKKFRIKARPSFVLRGLRALMENAQPTPELEKAVESEGENA
                     VALYATAAVYETVDRAKSSPWMEPLWTAEPETGAVPVAVTFFVATIGPGLEEELAAAL
                     TRGEGLRSQILTALGEESAEQAANFLTRLVGDEAKEEACELSPRFNVTVPELQRQILL
                     LLGSDKIQIQMDNVGHLSPRFTKIGHLLWWPPSKRRKPTEKK"
     gene            complement(2872..3774)
                     /gene="secF"
                     /locus_tag="P5079_08395"
     CDS             complement(2872..3774)
                     /gene="secF"
                     /locus_tag="P5079_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRY30037.1"
                     /translation="MEFFKTPNFDYISKRRIFFGLSGVLAVATVLSLVIKGPNFSIDF
                     TGGTLIQGFFKNPEPLEKVRAALDGAQLGGAELQSVPFHNAVIIRYKSGELGKEQMAS
                     QVTQALAKAFPDNPFEIERVEFVGPVVGQHLVKQAFYAILFSLLGIVIYVAFRFKNWI
                     WGFSGVFALAHDVFLTVGFMSITGREMSLTAIAALLTLAGYSINDTIVIFDRIRENLR
                     ARRKEPLDVLINRSCNETLSRTIITSLTVFMVLLSLLFFGGEVIRDFAAALTFGVVVG
                     SYSTIFIATPMVYLWQMKRSRPLR"
     gene            complement(3776..5209)
                     /gene="secD"
                     /locus_tag="P5079_08400"
     CDS             complement(3776..5209)
                     /gene="secD"
                     /locus_tag="P5079_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRY30038.1"
                     /translation="MPKVYWKALLVFVALVVGGIYLFPTFQWYRYSQADQEKMRIRRH
                     PLVEKILNLGLDLQGGIHLVLELKTDKLPDDKPETVREALDRAMEIIRNRVDQYGLAE
                     PLIVRQGEKWVVVQMPGVKDRDQAKSLIGRTALLEFRIVEMGEAIGPLQAKARELGLG
                     PENLRPGNMPKELTDMLPPGTELLPGKEENFYLVRATPEMTGSLLTNARVEMGGQYSG
                     LPVVSLEFSPEGAKRFADVTEANVQRQLAIVLDGIVQSAPVIRTRIPDGRAIIEGNFT
                     PEDAKLLKTVLQAGSLPAPLEIVEERTVGPTLGEDSIRAGVMACLIGLGLIFIFMMVY
                     YKWSGLLANAALVLNLFLLLGAMAAFHATLTMPGIAGIILTIGMAVDANVLILERIRE
                     ELRLGKTPRLAIDQGYDKAFSAILDGNLTTVIAALFLFQFGTGPVKGFGISLMLGLIV
                     SMFTAIIVTHTFYELLLMYKPIQRLSV"
     gene            complement(5215..5595)
                     /gene="yajC"
                     /locus_tag="P5079_08405"
     CDS             complement(5215..5595)
                     /gene="yajC"
                     /locus_tag="P5079_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRY30039.1"
                     /translation="MGIFAGMAAAPTLLCADSASPAAKPLGGGLVGVLPWVLIFVVFY
                     FLLIRPQQKQAQQQKKMIDALKKGDRILTQGGFYATVSAVKGKVLEVKLNDEVKVLVA
                     KSAVSQVVAGDPAQDIETPKPAVS"
     gene            complement(5610..>6377)
                     /locus_tag="P5079_08410"
     CDS             complement(5610..>6377)
                     /locus_tag="P5079_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY30040.1"
                     /translation="NTRTPTGGNPILVQFQSIAVAKKRCAAAEALGELGDQKFKYVLQ
                     LARRDRDPRVRDAVAIGLARLGDKTELIVFHSALNDKDDGVRLAAMEAVAKIAAPESQ
                     SRILAGLKDPSPEVRSAALRGLQRLKNPGVWRDVAALLRDENAMARESAAETLEIFQI
                     KEAVGPLKLLLEDENGYARAAAAKSLGRLGDRNGVDVLKSFMVSSDTDLCVRALQGLA
                     VFPDESEWGALARYLENAEDRVRLAAAVGIWKKGGLR"
BASE COUNT         1276 a         2043 c         1968 g         1090 t
ORIGIN      
        1 cgtccaccgc cggcataccg cgaaggactt ccgcgtaatt ttttttcacc agcaccgaga
       61 ggcgggccga agggaaggcc gccttgatgt tctcaaaaac cggcgcggcc aggatgatgt
      121 cgcccagcga cgacagccga acgacgagga cgctccgcgt cttttccgaa aaagccgaat
      181 tccgatccga tcccacgggc tcagccctcc gaacgaagcg gcaggatttt cgccaacaga
      241 tccaaatttc tctccacggc gccgcgctga gagtccgcgg cgcggcgggc ggcctctccc
      301 aaccggcggg aacggtcgcg gtcggcggcc aaggccctca tttccgacaa cagtccgtcc
      361 ccgtccgcca cccgaaccgc cgcccgttgt tccaaaaaca tcgcggccat ttcgcggaag
      421 ttctccatat ggggcccgaa gaggacgggc gctccccagc gcgccggttc caaaaggttc
      481 tgtccccccc gagggaccaa gctgccgccg acgaacaccc acgacgccag gccatagact
      541 tccggcaact cgccgatggt gtccagcacc attaccggcg tcgacatttc cggcgcggcc
      601 gaagcggaga attgagagcg cagggaatac gggacgtttt ccttttccag gcttcgcacg
      661 atgccgggga cgctctccaa atgccgcggc gcaatgacca gcttcacctc caccccctcc
      721 ttcaaaaggc cgtttcggac ctgaaggacg aattcggtct cgggaggatg gagactgccg
      781 cagacccaca cacggtcgtt ctcctggaga ccgtattttc ggaacaaagc cggacgggaa
      841 gcggcgtccc tcagaggaag gtcgtactta agattgccgg tcacgacgat cttctccctc
      901 tccgtgccga tacgggcgaa ccgctccgcg tggaccgggc tctgggcccc cacgacagcg
      961 aaccggctca acagcggccc ccagaaccat cgaagcagga ggtaactttt cagggaccgg
     1021 tcggaaatgc gcccgttgat gaggaggagc gggacgttga gatcggcggc ggtttggatc
     1081 cagttcggcc aaagttcggt ctccaccaac accagggcgg cgggacgcca ggcctcgagg
     1141 agtccccgca cgcacgcagc gaggtccacg ggcgcgaggc gcacctccgc cgtcggaaaa
     1201 agtttaaccg ccagttcttt cccggcgacg gtggtggtgg tcagtccgac gcgcaaggtc
     1261 tcaaaacggc tgaaaaaacc gcgcaaccac ggttccaccg cccggacttc cccgacggac
     1321 gccgcgtgca cccagaccag cggcgacgga tggacggagg ggcggcgggg aggcaaaaaa
     1381 cgttccggca ggccgcgcaa cgtccggcgc agaccgtaac gccagaccat gccgagaaca
     1441 acgagcggcc agaacagcaa aatgagaacg gaatagagga tcaacatatc gtttttccgg
     1501 cggggcgggt cttttcggag agcaactctt ccgccagccg tgccgccgca tcgagagccc
     1561 ggcggatttt ttcctccccg ccggcttctt ccggagcgat gtcgaccgga gcatgataaa
     1621 cgacctgata ccgcccgaag ggatacggga tcatgaaccg gtcccaggaa gacagcgcct
     1681 tcacgcgcct gccggcccag gccaccggaa gcacggggac gcccgtcttc tgggccgccg
     1741 ccacgacacc gggctggacg gccccgaggg gtccgcgggg cccgtccggc gtgaagacga
     1801 cgcgccggcc ggactccaaa tcccccaaaa cctccaacag ggccgccccg ccgcctcggc
     1861 tggaggaccc ccgggcggtg cggaacccca tccgccggag cggccgggcg accaattcgc
     1921 cgtcccggct ccggctcacc aagaccagaa tatcttcgtt ccgatggata tagatcgaca
     1981 ggagttggaa acggtgccag agggcgtaaa tgagcggacg gccggaccga aacaactctt
     2041 ccgcccgggg ctcgaaatgc cgttcgatgc gggacgttcg gccgatgaac tggagcactc
     2101 cccaacccaa ccaggagacg ccccacatca acacactgtc ccaccaacgc gggagcatgg
     2161 gtcttcagcg ggaggacgcc ggcgggaaag ccggtcggaa tttcatttct tttccgtcgg
     2221 tttgcgtcgt ttggagggag gccaccagag gaggtggccg atcttggtga agcggggcga
     2281 gagatggccg acgttgtcca tttgtatttg aattttgtcc gagcccagca ggagaaggat
     2341 ctggcgctga agttccggga ccgtgacgtt gaaacgcggg gagagttcgc aggcctcctc
     2401 tttggcctcg tcgccgacga ggcgggtgag gaaattggcc gcctgttcgg cgctctcctc
     2461 gccgagggcc gtcaagatct ggctccgaag cccctcgccc cgggtcaaag cggcggccaa
     2521 ctcctcctcc aaacccggcc cgatggtggc gacgaagaag gtcacggcca cagggaccgc
     2581 ccccgtctcc ggttcggccg tccagagcgg ttccatccat ggcgacgatt tggcgcggtc
     2641 gacggtctca tagaccgcgg cggtggcgta gagagcgacg gcgttctccc cctccgactc
     2701 cacggccttt tccagttccg gcgtgggctg ggcgttttcc atcaacgcac gcagtccgcg
     2761 caagacgaaa gaaggacgcg ctttaatgcg gaattttttg atctttttca tttggaattt
     2821 cgtggcgcgc gagcccgccg gcggcgcatg aaaagaagcg tggtcagcga atcaccgcag
     2881 cggacgggac cgtttcatct gccacagata gaccatgggg gtggcgatga aaatggtgga
     2941 ataggacccc accaccacac cgaaggtcag ggccgcggcg aaatcacgga tcacctctcc
     3001 gccgaaaaac aggagcgaca gaagcaccat gaacaccgtg agggaggtga tgatggtccg
     3061 cgagagcgtc tcgttgcagg aacggttgat gagcacgtcc agcggttcct tgcgccgggc
     3121 ccggaggttt tcccggatgc ggtcgaagat gacgatggtg tcgttgatgg aataaccggc
     3181 cagcgtcagg agggcggcga tggcggtgag agacatctcg cgtcccgtga tggacatgaa
     3241 cccgacggtc agaaacacgt cgtgcgccaa cgcgaacacg ccggaaaatc cccatatcca
     3301 gttcttgaac cggaacgcca cgtagatgac gatgccgagc agggagaaga ggatggcgta
     3361 gaacgcctgt ttcaccaggt gttgcccgac caccggcccc acgaactcca cccgctcgat
     3421 ctcgaagggg ttgtccggaa acgccttggc cagcgcctgc gtcacctggg aggccatttg
     3481 ttccttgccc agctcgccgg acttatagcg gatgatgacg gcgttgtgga acggaacgct
     3541 ctgaagttcg gcgccgccca gttgggcccc gtccaacgcg gcccggacct tctccaacgg
     3601 ttccgggttc ttgaaaaaac cctggatcag ggtcccgccc gtgaaatcga tgctgaagtt
     3661 gggccccttg atcacgaggg acaggacggt cgccacggcc agaacgcccg agagcccgaa
     3721 aaaaatccgg cgtttgctga tgtaatcgaa gttgggggtt ttaaagaatt ccatattaga
     3781 cactcagcct ttgtatgggt ttgtacatca acaacagttc gtagaaggtg tgcgtgacga
     3841 tgatggccgt gaacatggaa acgatgagac ccagcatgag gctgatgccg aaccccttca
     3901 ccgggccggt gccgaactgg aagaggaaga gcgcggcgat gacggtggtc aaatttccgt
     3961 cgaggatggc ggagaaggcc ttgtcgtagc cctggtcgat ggccaggcgg ggcgtcttcc
     4021 ccagccggag ttcctcccgg atgcgttcca agatgagcac gttggcgtcc accgccatgc
     4081 cgatggtgag gatgatgccg gcgatgccgg gcatggtgag cgtggcgtga aaagcggcca
     4141 tggcccccag gagcaggaaa aggttcagca cgagcgccgc gttggcgagc aggccggacc
     4201 atttatagta gaccatcatg aaaataaaga tcagtccgag cccgataagg caggccatga
     4261 cgccggcgcg gatggagtcc tctccgaggg tcgggcccac ggtgcgttcc tccacgatct
     4321 ccagcggggc cgggagggag ccggcctgca gtaccgtctt gaggagtttg gcgtcttccg
     4381 gagtgaaatt cccctcgatg atggcgcggc cgtccgggat gcgggtgcgg atgaccggcg
     4441 ccgactgcac gataccgtcc aaaacgatgg ccaactgccg ctgaacgttc gcctcggtca
     4501 cgtccgcgaa ccgcttggcc ccttccgggc tgaactccag ggaaaccacc ggcagccccg
     4561 aatactggcc tcccatctcc acgcgggcgt tggtgagcaa ggaaccagtc atctccgggg
     4621 tggcgcggac gagataaaaa ttctcctcct tgccgggcag aagctccgtc cccggcggca
     4681 gcatgtccgt caattctttc ggcatgttgc cggggcggag gttctccggc cccaggccca
     4741 gctcgcgcgc cttggcctgg aggggcccga tggcctcgcc catctccacg atgcggaact
     4801 ccaaaagcgc cgtgcggccg atgaggcttt tggcctggtc gcggtctttc acgccgggca
     4861 tctgcaccac gacccacttt tcaccctggc gcacgatcag cggttccgcc aaaccgtatt
     4921 ggtccacgcg gttacggatg atctccatgg cccggtccag ggcctcgcgg acggtctccg
     4981 gtttatcgtc gggaagcttg tccgtcttga gttccagaac caggtggata ccgccttgca
     5041 ggtccaggcc gaggttcaag atcttttcca ccaaaggatg ccggcggatg cgcatctttt
     5101 cctggtccgc ctgggaatac cggtaccact ggaaggtcgg gaagagataa atccccccca
     5161 ccaccagggc gacgaaaacc aacagggctt tccaatagac tttcggcatg ataattaaga
     5221 aacggccggt ttcggcgttt caatgtcctg ggccgggtcg ccggccacca cctgggacac
     5281 cgcggacttg gccaccaaca ccttgacctc gtcgttgagc ttcacttcca aaacctttcc
     5341 tttgacggcg ctgacggtgg cgtagaaacc gccctgagtc aggatgcggt cgcctttttt
     5401 caaggcgtcg atcatcttct tctgttgttg cgcctgtttc tgctggggac ggatgagcag
     5461 aaaatagaaa accacgaaaa tcaggaccca cggcaacacg ccgacgagtc cgccccccaa
     5521 aggtttcgcc gccggagaag ccgaatcggc acagagcaag gtcggcgccg ccgccatgcc
     5581 cgcaaaaatt cccatccatt tttgatgaat caccgtaacc ctcccttttt ccatatcccc
     5641 acggcggcgg ccaatcggac ccgatcctcc gcgttttcca aataacgggc gagagcgccc
     5701 cactccgact cgtccggaaa cacggccaat ccctgcaagg cccggacgca caaatccgta
     5761 tcgctgctga ccatgaaact tttcaacacg tccacgccgt tgcggtcgcc gaggcggccg
     5821 agggatttgg cggcggcggc gcgggcatag ccgttctcat cctccaagag cagcttgagc
     5881 gggcccacgg cttcctttat ctgaaaaatc tccagcgtct ccgcggcgga ttcccgcgcc
     5941 atggcgttct catcgcgaag gagcgcggcc acatcgcgcc acacgcccgg attcttcagc
     6001 cgctgcaacc cgcgcaaggc ggcggagcgg acctccgggg aggggtcctt caaccccgcc
     6061 aatatccgcg actggctttc cggcgcggcg atcttcgcca ccgcctccat ggccgccagc
     6121 cgcaccccgt cgtccttgtc gttgagggcg gaatgaaaga cgatcagttc cgtcttgtca
     6181 ccgagccggg cgaggccgat ggccaccgcg tcgcgcacgc gcgggtcccg gtcgcggcgg
     6241 gccagttgga gcacgtattt aaatttctga tcacccagct cgcccagggc ctcggccgcg
     6301 gcgcaccgtt tcttggccac cgcgatggac tgaaactgaa ccaagatcgg gttcccgccc
     6361 gtcggcgtgc gcgtgtt
//
LOCUS       DAOTFD010000148         6374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8718_length_6374_cov_5.027061, whole genome shotgun sequence.
ACCESSION   DAOTFD010000148 DAOTFD010000000
VERSION     DAOTFD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8718_length_6374_cov_5.027061"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(768..1076)
                     /locus_tag="P5079_08415"
     CDS             complement(768..1076)
                     /locus_tag="P5079_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30041.1"
                     /translation="MNIGFKNPFRDRRLLFVGHKAAIDDFVAVWRMAAIVEAAFCPLR
                     KFIPNPLRSQLPFKLRECKHDVTEQPTGRGRSVETLSHRYIADVMGLQNFDQLVKIAR
                     "
     gene            complement(1936..2284)
                     /gene="ssrA"
                     /locus_tag="P5079_08420"
     tmRNA           complement(1936..2284)
                     /gene="ssrA"
                     /locus_tag="P5079_08420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2304..2639)
                     /locus_tag="P5079_08425"
     CDS             complement(2304..2639)
                     /locus_tag="P5079_08425"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY30042.1"
                     /translation="MISDQRHHHRVSFFVGAGVSSNLEGSDSVRAVIRDISVSGLRIE
                     TLAKFEEGRTVYLDFNVAGRFQFTKMPAVVKRVYRHGGGFLLGLMMQDGTDRRRVRQA
                     LTYVMENAL"
     gene            complement(2636..3106)
                     /gene="tadA"
                     /locus_tag="P5079_08430"
     CDS             complement(2636..3106)
                     /gene="tadA"
                     /locus_tag="P5079_08430"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRY30043.1"
                     /translation="MSAEKDAKRFMRLALREARKAARADEVPVGAVLVRDGRVLAADR
                     NRIRARRDPTAHAEMLVLRAAARRLKNERLGDTILYTTVEPCPMCAGAIVLARVREVV
                     FGTADPKAGAGGSLIDILQHPQLNHRVRVSGGFYGQEARRQLRRFFRKKRALKK"
     gene            complement(3103..3588)
                     /locus_tag="P5079_08435"
     CDS             complement(3103..3588)
                     /locus_tag="P5079_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HRY30044.1"
                     /translation="MSDGPRFLADAMLGRLARWLRLLGYDTAYDPGAADEELLHRALR
                     EGRVLLTGDGGISRRKSLRLLLLRQKTLEDQLRRVMAEFRLRPKEELFFSRCPVCNEP
                     LAPAPKEKLRERVPERVFERQDDFYRCAACEKIYWQGSHSRKVREFVKRLENEGGGEG
                     R"
     gene            complement(3585..4829)
                     /locus_tag="P5079_08440"
     CDS             complement(3585..4829)
                     /locus_tag="P5079_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRY30045.1"
                     /translation="MSPYALFFLAALLLIHAVDVFSRWLNSRSLDPRLPEEFQGVFGA
                     EAYRRSQEYERTKTRFDVLQSSAGLAVLLVFWFVGGFERLDAWVRGWALSEVLTGLSY
                     IGLLFLANEAVSLPFEVYRTFVIEERFGFNKTSPRLFAADRLKSYGLAALIGGPLLAL
                     ILFFFGWAGDAAWAWAWGAAAVFRVLLQLVGPTWIMPLFNKFTPLPDGELRDAIFRYA
                     ASVGFPLRNIFVMDGSKRSSKTNAFFSGFGKNKRIALFDTLIDKHAVEELVAVIAHEI
                     GHYKRGHVWKGMALSLANYGLLFYLLSLFVRAPVLFEAFGVTRASVYVGLLLFGICYA
                     PVSFLLAIATQAFSRRNEYQADRYAAETVADRNALVRALKTLSVSNLSNLTPHPLFVF
                     LHYTHPPVLRRIAALRAAPSAG"
     gene            complement(4854..6107)
                     /locus_tag="P5079_08445"
     CDS             complement(4854..6107)
                     /locus_tag="P5079_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY30046.1"
                     /translation="MPKSEAFPEIEETSWKRPLWALLLMQFLAMLGMSQFLPFLPLYV
                     QSLGVREAAEAASWSGLLFGIGFLFAGFMAPVWGYMADRWGRKRMVIRATLGSGLALC
                     FMAYARDVNDLLILRILHGSLGGFVAANTALVATLVPELRLGYAMGALQSMTMLGLIL
                     GPFVGGFLVDWWGGFRYVILFSAMLVLVGALVVAVMVPDDKIRLKEGIHLSIFENLRF
                     VISSPMLLTASGIQFVVQVALMGIQPVLALLIQELHVSERYVGSLTGVIFGITGVTTL
                     IGAFALGRHGDKRGAIPVLQGCLLGAALVYIPQGFVTGVLSLLLLRAALGFFVGGIQP
                     ATQSLVARGAPLERRAGVLGISFSGTLFGNALGPIIGGAVAGFAGLRAPFLLTAILLL
                     IAWGISRRYMVRREDMEPVLEETAL"
     gene            complement(6110..>6374)
                     /locus_tag="P5079_08450"
     CDS             complement(6110..>6374)
                     /locus_tag="P5079_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY30047.1"
                     /translation="SAIHDAGLSVPGHVAVVGFDDLEMPALTFRVPSLTTVRQPIYEI
                     AKEGTESLILLVEGRASAPRQKVFSSQLIVRSTCGSSLKKTKG"
BASE COUNT         1395 a         1989 c         1902 g         1088 t
ORIGIN      
        1 gatattcggg aaaggcaagg ggacagttcg acggctttca aaagaggcca ccaatttccc
       61 tgtatcgaac cggggaatgg ggctggacga ctcaacatcg tccggccctt tttggttgtc
      121 cgaaccctgc accgaaacag cggctaccgc gcgaagcgcg gaggcggctg aacccactgg
      181 atttggacag ttgccgtcga gcgagaactg gtagtcaaaa tcaattgtga ttgtcttttt
      241 ggaatagcgg attttttcga tgcctcggcg aggggcttat cagccccact ccataaaccg
      301 gagcacggcc aaaccctttt caacccaatg cgcgcgcaca gaggtggttt ctatttacca
      361 ccctcaccct gctcccttcc gcaagagctt cagggacgga ggattggcca gcggcctttg
      421 ccgcctcctc cctccagggc gagggtgaaa aagacaaaag gagatttcta attatgaaaa
      481 acaaaactga aaacccaacg gcaaaaaaac tgacccgttg cgctgtttat actcgcgttt
      541 ccacagacca acaagcggaa gtggagttta actcctgcga ggcacaggaa gaccgcatcc
      601 gctcctttat ttccagccaa caggatttca tcctttcaaa ggtttactcc gatcccgggt
      661 acactggcgc gaataccgac cggccgggac ttcagcggtt gctaaccgac gtgaaagcgg
      721 gcctcgttga tatggtcttc acctacaaaa ttgaccgcct tacacggtca ccgcgcgatt
      781 tttaccagtt gatcgaaatt ttggaggccc atcacgtcgg ctatatatcg gtgactgagc
      841 gtttcgacac ttcgaccccg gccggtcggt tgctccgtaa catcatgctt acattcgcgc
      901 agtttgaacg ggagttggct tcggagcgga ttcgggataa atttgcgcaa cgggcaaaac
      961 gcggcctcta caatggcggc catccgccat acggctacaa aatcatcaat ggccgccttg
     1021 tgcccgacaa aaagaaggcg acggtcacga aatggatttt tgaaacctat gttcaaaccc
     1081 gttccctccg ccaagttcaa aaccttctag cgggaaaagg tgtcctctca aaaactggcc
     1141 ggatgcatgg caattctttc ctttggcatc tgctacggaa ttctgttgtg accgggaggg
     1201 ttccatacaa aggacagttt tatccgggtc agcacccggc cattgtttca gaggaactat
     1261 ttaatcacgt tcaaaaacta ctgacagagg aaccccgccg agcgccccag ccgtatcaac
     1321 atcttccttt cgccgggctc attcattgcg aggagtgcgg ctcgaccatg agcccgtgcc
     1381 acacgaacaa ggtgaacaag tccggcccca gaaaatatta ttactaccga tgcacctccg
     1441 tgacgcatca cggctggggc gcttgctcca cccgccaaat cagcgcggag cgttttgaag
     1501 acatcatcca caaaaatttg ctccgcgttt ccttggacgg cgactattta aaaaaccatg
     1561 ttttcgccct ccaaaacaaa acccgccacc atgaggtgac gggaatcgaa ccggaaggaa
     1621 aatacgcgga tttgaccgcc gaaaatcttc aaaaatcact gcaaaacttc ttaaaggcgt
     1681 gcgcgcgcaa aacggggatt gaaaagactt tggccgttcg ccgaggcatc gaaaaaatcc
     1741 gctattccaa aaagacaatc acaattgatt ttgactacca gttctcgctc gacggcaact
     1801 gtccaaatcc agtgggttca gccgcctccg cgcttcgcgc ggtagccgct gtttcggtgc
     1861 agggttcgga caaccaaaaa gggccggacg atgttgagtc gtccagcccc attccccggt
     1921 tcgatacagg gaaattggtg gaggtggcgg gaatcgaacc cgcgtccgaa agtgtgtcga
     1981 ccaggccgct acaggcttag cctggagttt gtctcgcggg aaggttgtct ccagacggaa
     2041 ccgccttccc gccagttccg ttgttgtctc gtcctacgcc gacgaaacgc gcggcgcggg
     2101 accagccggc tgtcggcgcc cgttccctcc agccggcatc aagggacgga cgtcacggcg
     2161 tattaggccg cgagggccaa ctgttcgttg gcagttgtgg ttttgccggt ttttttacga
     2221 agctccgacg acttcggcct gcaaccaggg tctcctgcac ttccgtcgaa cccatttcac
     2281 ccccaaaacg gtcgcttccg atatcagagg gcgttctcca tcacgtaggt gagggcttgg
     2341 cgcacgcggc gccggtccgt gccgtcctgc atcatcagcc ccagcaggaa ccctccgccg
     2401 tggcgataga cccttttgac caccgccggc atcttcgtga actggaaccg gccggccacg
     2461 ttgaaatcca gatagaccgt gcggccttcc tcgaacttcg ccaacgtctc gatccgcaag
     2521 ccggagacgc tgatgtcgcg gatgacggcc cggacgctgt cgctcccctc gaggttggag
     2581 gagacgccgg cgccgacgaa aaaactgacc cgatggtggt gccgctggtc cgatatcatt
     2641 ttttcaaagc ccttttcttc ctgaaaaacc gacgcaactg ccggcgggcc tcttgtccat
     2701 agaacccgcc ggaaacacga acgcgatggt tgagctgggg atgctgcagg atgtcgatca
     2761 gagagccccc ggcgccggcc ttgggatccg ctgtcccgaa cacgacctcg cgcacgcgcg
     2821 ccagaacgat ggccccggcg cacatggggc acggttcaac cgtggtataa agtattgtat
     2881 cacccaaccg ctcgtttttc aaccgccggg cggccgcgcg caacaccagc atttcggcat
     2941 gggcggtggg gtcgcggcgg gcgcggatgc ggttgcggtc cgcggccaaa acccggccgt
     3001 cccgcaccag cacggcgccc acggggacct cgtccgcccg ggcggccttc cgcgcctccc
     3061 gcagggcaag ccgcatgaaa cgtttcgcgt ctttttccgc gctcatcgtc cttcccctcc
     3121 cccttcgttc tccagccgct tcacgaactc ccgcaccttc cgggaatggc tcccttgcca
     3181 gtatatcttc tcacaggccg cgcagcgata gaaatcgtcc tgcctctcga acacacgctc
     3241 cggcacgcgc tcccgcagtt tctccttggg cgccggcgcc agcggctcgt tgcagacggg
     3301 gcagcgggag aaaaaaagtt cctccttcgg gcggagccgg aactccgcca tcacgcgccg
     3361 aagctggtcc tccagggtct tctgtctcag gaggaggaga cggagacttt tccttcggct
     3421 gatccccccg tcgccggtga gcaggacccg cccttcccgg agcgcccggt ggaggagctc
     3481 ttcgtcggcg gccccgggat cgtaagcggt gtcgtatccc agaagacgca gccagcgggc
     3541 cagccggccc agcatggcgt ccgccaggaa acgggggccg tcgctcatcc cgccgatgga
     3601 gcggcccgga gggcggcgat cctccgcagg acgggcggat gcgtgtaatg caggaagacg
     3661 aagaggggat gcggcgtgag gttgctcagg ttggagacgg agagcgtctt caaggcgcgg
     3721 acgagggcgt tccggtcggc gacggtttcg gccgcatagc ggtcggcctg gtattcgttc
     3781 cgccgggaaa acgcctgggt ggcgatggcg aggaggaacg agaccggcgc gtaacaaatg
     3841 ccgaaaagca gaagccccac gtacacggac gcgcgcgtca ccccgaacgc ctcaaagagg
     3901 accggggcgc ggacgaacaa cgagaggagg tagaaaagca ggccgtagtt cgccaggctc
     3961 aacgccatcc ccttccacac gtgccccctc ttgtagtgac cgatctcatg ggcgatgacc
     4021 gcgaccagtt cctccaccgc gtgcttgtcg atcaacgtgt cgaagagggc gatccgtttg
     4081 ttcttcccga accccgagaa gaaggcgttc gtcttggaag agcgtttcga gccgtccatg
     4141 acgaagatgt tccgcagagg gaaacccacc gaggcggcgt agcggaagat ggcgtcccgg
     4201 agttccccgt cgggcagcgg ggtaaatttg ttgaaaagcg gcatgatcca cgtgggcccg
     4261 acgagttgaa gaagaacgcg gaagacggcg gcggcccccc acgcccacgc ccaggcggcg
     4321 tcgccggccc atccgaaaaa aaagaggatc agcgccagca aaggaccgcc gatcagcgcg
     4381 gcgaggccgt aactcttcag ccggtccgca gcgaacagac gcggggaggt cttgttgaaa
     4441 ccgaaccgtt cctcgatgac gaaggtccgg tagacctcaa aagggaggga gacggcctca
     4501 ttggcgagga aaagaagtcc tatataggaa aggccggtca ggacctccga cagcgcccac
     4561 ccccggaccc acgcgtccag ccgctcgaac ccgccgacaa accaaaagac cagcagcacc
     4621 gccaacccgg cggaggattg aaggacgtcg aaacgggtct tcgtcctctc gtattcctgg
     4681 gaacgacggt acgcctcggc gccgaaaacg ccctgaaact cctctgggag acgcgggtcg
     4741 agggaacggc tgttgagcca acggctgaag acgtcgacgg cgtggatcag caggagggcg
     4801 gcgaggaaga agagggcgta gggcgacatg gcgggcaaaa gggcgccggt gtgttaaagg
     4861 gccgtttcct ccagcaccgg ttccatatcc tcccgtcgaa ccatgtaccg gcgggaaatg
     4921 ccccaggcga tcaaaagcag gatcgccgtc aggaggaaag gcgcccgcag gccggcgaag
     4981 cccgccacgg ccccgccgat gatgggcccc agcgcgttgc cgaagagggt gcctgaaaaa
     5041 gagatgccca gcacccccgc gcggcgttcc agcggcgcgc cgcgggccac caggctctgc
     5101 gtggcgggct gaatgcctcc cacaaaaaac ccgagcgcgg cgcgcagcag caacaaggat
     5161 agaacgcccg tgacgaaccc ctgcgggata tagaccagcg ccgcccccag aaggcatccc
     5221 tggaggaccg ggatggcgcc gcgtttgtcg ccgtgtcggc ccaaagcgaa cgcgccgatg
     5281 agcgtcgtca cgcccgtgat gccgaagatc acgcccgtca aactgccgac atagcgctcg
     5341 gaaacatgca gttcctggat gaggagcgcc aacacgggct ggatgcccat cagcgccacc
     5401 tgaaccacga actgtatccc cgaagccgtc aacagcatcg gcgaggagat cacgaaccgg
     5461 aggttctcaa aaatgctgag atggatcccc tccttgaggc ggatcttgtc gtcagggacc
     5521 atcacggcca ccaccagggc gccgacgagc accaacatcg ccgagaagag gatcacgtaa
     5581 cggaacccgc cccaccaatc cacgagaaaa ccgcccacga acggccccag gatgaggccc
     5641 agcatggtca tggactgaag cgcccccatg gcgtaaccga gacggagttc cggcaccagc
     5701 gtggccacca acgccgtgtt ggcggcgacg aaaccgccga gactgccgtg caatatcctc
     5761 aggatgagga ggtcgttgac gtctcgcgcg taggccatga aacacagcgc cagccccgaa
     5821 ccgagcgtgg cgcggatgac catccgcttc cggccccagc ggtcggccat gtagccccac
     5881 accggggcca tgaatccggc gaaaaggaat ccgatgccga agagaagacc gctccaagaa
     5941 gccgcctccg ccgcctcccg gacgcccagg gactggacat acagcggcag gaacggcaga
     6001 aactgcgaca tccccagcat ggccaggaac tgcatgagga ggagcgccca caaggggcgc
     6061 ttccaagacg tctcttcgat ctcggggaag gcttcggact tcggcacgat cagccttttg
     6121 tttttttgag ggaggacccg caggtgctgc ggacgatgag ctggctggaa aaaactttct
     6181 gccggggcgc gctcgcccgg ccctcgacca ggaggatgag cgactcggtg ccttccttgg
     6241 cgatttcgta gatgggctgc cggaccgtgg tgagggacgg cacgcggaag gtgagcgcgg
     6301 gcatctccag atcgtcgaac cccaccaccg ccacgtggcc gggcaccgag aggccggcgt
     6361 cgtggatggc ggag
//
LOCUS       DAOTFD010000149         6353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8762_length_6353_cov_3.989520, whole genome shotgun sequence.
ACCESSION   DAOTFD010000149 DAOTFD010000000
VERSION     DAOTFD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_6353_cov_3.989520"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="P5079_08455"
     CDS             complement(<1..1030)
                     /locus_tag="P5079_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30048.1"
                     /translation="MKLKEIVHRWWSEVSTLFRRLVQSVDGTSLSSARFPFGDGWSAP
                     SFVKVVTNSTRQTGASFYFFARLLVVSVLIAQLATGAWADAPGVLNYQGKLTNQDGSP
                     ITATPTMEFRIYDASTGGTLLYTYGSLTVPVDNGVFNVQIGSNTAVSGGAIPKTVFQG
                     GTDRWLEIVVAGETLSPRERLTAAPYALAVATNSVGANEIVNGSVAIGDLNVASVETI
                     FLSTGTPQTITATKTFSGATLLGLPNPPPQADAAASKAYVDAQVTGSGGWSRDSGNSE
                     VELSNVGDQVGIGTSAPDRKLHIASTNDQLVIEDTDAGTDYKKWMLKADGATMELVTA
                     DDGFNNFQA"
     gene            complement(1248..1874)
                     /locus_tag="P5079_08460"
     CDS             complement(1248..1874)
                     /locus_tag="P5079_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30049.1"
                     /translation="MSKEAGLLKISEIAEKGSVLPSTIRHYTDLGLLQYAATTDGGHR
                     LYHEQETLTQLARIKRLAARGLSLPDIKAELTGKGKKRILVVDDEQEVCELVNDALKD
                     RHPFDIRIVHDGFSAGRILADFIPDLVILDLMLPGLDGFHVCRQIRADQMLLGAKILA
                     VTGYDTPENTRKILDAGADKLLTKPLDTRELLKAVEELLNITMTVRAV"
     gene            complement(2583..3548)
                     /gene="dusB"
                     /locus_tag="P5079_08465"
     CDS             complement(2583..3548)
                     /gene="dusB"
                     /locus_tag="P5079_08465"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRY30050.1"
                     /translation="MKPSAVPPVRLGPVLLDGPFVLAPMAGVSDPPFRRLCRRNGASL
                     VCAEMVSANALHFKDKRSQRMLEVFPDEHPLSLQVFGSDPDRLAKAAKMAAAAGADIV
                     DLNCGCPVPKVMRTGAGLALTKDEKNLARCLEKMVSAVNVPVTAKFRLGARRGRNDAV
                     RLARLAESCGVAAVTVHARWKDAGHSGPPDLEALHDVAAEVRIPVLGNGGVQTAADAR
                     RMIETGCVGVMVGQAAMGNPLIFRELWEGEKAKPFSERGVFDLLLEHARMNVAYFGEK
                     LGIVRLRKVMGAYIKNFPQAATFRHRAYQVETLSELVELLNQVRP"
     gene            complement(3556..4617)
                     /gene="tsaD"
                     /locus_tag="P5079_08470"
     CDS             complement(3556..4617)
                     /gene="tsaD"
                     /locus_tag="P5079_08470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY30051.1"
                     /translation="MTDSKDLRVLGIETSCDETAAAVVEGGRRILSNVIASQVAIHQP
                     YSGVVPELASRAHVGNIQTVVQEALKGFGGRGQFPFPKTLPVDVIAATMGPGLVGSLL
                     IGKIAAEMFAWAYQKPLVGVNHLEGHVLSVLPANPDLHPPFLALVTSGGHTELVHVKN
                     YGQFVVLGRTRDDAAGEAFDKVAKALELGYPGGPIIDRLAAKGRATAIPFPRPFLGSG
                     WDFSFSGLKTAVIYHLEKNPGVKESKKKVADVCASFQAAVIDVLVGKTLAAAEHLGLK
                     NIVVTGGVAANSALRTAFLNRAKKQKIHMASPLLCTDNAVMIAVAGYYKYQKSPHKKF
                     MPHALVVDSTLCIQDWHCH"
     gene            complement(4731..5729)
                     /locus_tag="P5079_08475"
     CDS             complement(4731..5729)
                     /locus_tag="P5079_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30052.1"
                     /translation="MGTEGKSEPPFQPWWKEAFDGGPSFTWDLHNLCNYRCSYCWWEQ
                     ADQWQYFDKKDHPIPWQAWLRPWSRMNEKYGRCRLHVLGGEPLLYPGVGDLFAELSKH
                     HYLYVATNLSSSLEELKKMTARWDASAIYLNASFHPEFAAPDLFLKKLLYLRSLGFTP
                     SAAMVSYPPHIPQMTRIRELLAKEGIPITFQVFQGKYQGKSYPESFSVEERALLDLEP
                     GTRPAGEGQPVAPVEQPSLKGKPCCAGWFYANVKADGEVFRCGGTGPGMEAEARLGNL
                     FGDDFELLPAPAPCPARHCFCQENIFLLEAQEKKQPEFLKAWARKTNAARGAKVGG"
     gene            complement(5989..>6353)
                     /locus_tag="P5079_08480"
     CDS             complement(5989..>6353)
                     /locus_tag="P5079_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30053.1"
                     /translation="ALLRRLLPSRKAGLSGDFVKDSAWLQRTTRDLLAANGLQDGQVL
                     KSFNQEREMDGIRWLEDTLEMDYPAGFNSARFLKQLGSVLPKKGYRLVRNDKTDKKWI
                     LELGASEDVVFQKLVFHI"
BASE COUNT         1281 a         1945 c         1809 g         1318 t
ORIGIN      
        1 cggcctgaaa gttgttgaag ccgtcgtcgg cggtgaccag ttccatggtg gccccatcgg
       61 ctttgagcat ccacttctta taatcagtgc ccgcatcggt gtcctcgatg acaagctgat
      121 cgttggtcga tgcgatatgg agtttgcggt ctggagcgga ggtccctata cccacctggt
      181 cgccgacatt gctcagttcc acctcgctgt tgccgctgtc ccggctccac ccgcccgacc
      241 ccgtcacctg ggcatccaca taggccttgg aggccgcggc atccgcctgc ggcggcgggt
      301 tgggaagacc gaggagggtg gccccgctga aggttttggt ggcggtgatg gtctgcggcg
      361 tgcccgtgct gaggaagatg gtttccacac tggccacgtt caaatcgccg atggccacgc
      421 tgccgttcac gatctcgttg gcgcccacgc tgttggtggc caccgccaga gcgtacggag
      481 ccgcggtgag gcgttcgcgc ggcgagaggg tttcccccgc cacaacgatt tcgagccagc
      541 ggtcagtgcc cccctgaaaa acggtcttgg ggatggcccc cccggaaacc gcggtattgg
      601 aaccgatctg cacgttgaac accccattgt ccacgggaac cgtcaaagaa ccgtacgtat
      661 agagcagggt tcccccggtg gaggcgtcat aaatgcggaa ctccatggtc ggggtggccg
      721 tgatcggcga tccgtcctgg ttggtcaatt tgccctggta attgagaacg cccggggcat
      781 ccgcccaggc gccggtggca agttgcgcaa taagaacaga gactactagt aacctcgcga
      841 aaaaataaaa agaagcgccg gtttggcgcg tcgagttcgt gacaactttc acgaaggagg
      901 gcgcactcca cccgtcgccg aacgggaagc gcgcactcga aagagacgtt ccgtccacgg
      961 actgaaccaa ccgacggaac agcgtcgaga cttccgacca ccaacgatga acgatctctt
     1021 tcaatttcat ggcgataccc tcctgcgatt tagatgcatg ttcggcgaat gatgaaaatc
     1081 cgatgacact ttccgaaaac cgaacgtttc ggacggcggt gagacaaaaa aaacgggaca
     1141 agggcgatga agccccttcc cgtttgcgat tttttcctcg tgaatcagac tcggcttctt
     1201 cttcacgatg aactccttgg ctaccgaatg tttttaagat tccccttcta aacggcccgg
     1261 accgtcatgg tgatgttgag caattcctca acggccttga gcaactcccg cgtgtccagc
     1321 ggcttggtca acagtttgtc cgcccccgcg tccagaatct tgcgggtgtt ctccggcgtg
     1381 tcgtagccgg tgacggccag gattttcgcc cccagcagca tctggtccgc gcggatctgg
     1441 cggcacacat ggaaaccgtc caggccggga agcatcaggt ccagaatgac caggtccgga
     1501 atgaaatccg ccaggatccg tccggcggag aacccgtcgt gcacgatgcg gatgtcgaaa
     1561 ggatggcggt ccttcaacgc gtcgttaacg agctcgcaca cctcttgttc gtcgtcgaca
     1621 accaggatgc gcttcttccc tttgccggtc aattccgcct tgatgtccgg cagggagagc
     1681 ccgcgggcgg ccaaccgttt gatgcgggcc aactgggtca aggtttcttg ttcgtgatag
     1741 agtcgatgac ctccgtcggt ggtggcggca tactgcaaaa ggccgaggtc ggtgtaatgg
     1801 cggatggtgg agggcaacac gctccctttt tccgcaatct cactgatttt gaggaggcct
     1861 gcttctttcg acatcttttt tgtcctttcg gaaccgtcac ttattactac aagagaatcc
     1921 tgaaaaaaaa cacctgccct cttcaagcgc tttaattggc tttgaatttt ttcccatttg
     1981 gaccgatttg tcaacgggaa aaaacccttt tttcatgaaa aagaacgcgc actcataacc
     2041 atcaaccgtg ctgcgaactt catgttttaa acaataaaaa atgcggaact ttacggttcc
     2101 gctttaaagt taacccctta aacgcctttt gtcaagggtt tttttattca tccccccgga
     2161 tgtgcgattg tgcgttattt tcgatatttc acccccccct cgttccatac caatcctcgg
     2221 tttaagcata tcgggtggtg actatccctt ggttgcacgt atcttctcga ccccctttcc
     2281 cttcctacaa acagggatca tccctagcgg caggggggcg ctcgaagaat aactgtattg
     2341 ctcgagcgga gcgaagtcga tcccaacctt gcctggaaag aattaacgcg ggatcgtccc
     2401 tcttcagaca gtggttggga cgagaaccga gaaggctaaa atcgggttcc ttgttgtcca
     2461 ggaatgctcg cccatagggt tctcttcgaa cggttccatc gggctttcag attccccgaa
     2521 aaacaattcc cggtgaacgt gcgcggaagg agcggcggcg cgtggcgccg tcgagagtgt
     2581 cttcaggggc ggacttggtt caacagctcg accaattccg aaagggtttc cacttgatag
     2641 gccctgtgac ggaacgtcgc cgcttgggga aaatttttga tgtaggctcc catcactttc
     2701 cgcaatcgca cgatgccgag tttctctccg aaatacgcca cgttcatccg cgcatgttcc
     2761 aacagcagat caaaaacccc gcgctcggaa aatggtttgg ctttctcccc ctcccacaat
     2821 tcccggaata tcaaagggtt tcccatggcg gcctggccca ccatcacgcc gacgcatccg
     2881 gtctcgatca tccgtcgggc gtcggcggcg gtttgcaccc cgccgttgcc cagcacggga
     2941 atcctcactt cggcggccac gtcgtgcaac gcctccaagt cgggcgggcc ggaatgaccg
     3001 gcgtctttcc agcgcgcgtg gacggtcacg gcggccaccc cgcaggattc cgccaagcgc
     3061 gccagccgga cggcgtcgtt gcggccccgc cgcgcgccga gacggaactt ggcggtgacg
     3121 ggaacgttca ccgccgacac catcttttca agacatcgcg ccaggttctt ctcgtccttg
     3181 gtcagggcca gccccgcccc cgtacgcatc accttgggca cgggacagcc gcagttcaag
     3241 tccacgatgt ccgccccggc cgcggccgcc atcttcgccg ccttcgccag gcgatccggg
     3301 tcggaaccga acacctgcag actgagcgga tgctcgtcgg gaaacacctc cagcatgcgt
     3361 tgagagcgct tgtccttgaa atgcagggcg ttggcggaca ccatttcggc gcacaccagg
     3421 gacgccccgt tgcggcggca gagacggcga aacggaggat cgctgacccc cgccatggga
     3481 gccaacacga acggcccgtc gaggagcacc ggaccgaggc gcacgggagg gacagcggaa
     3541 ggtttcaagg acaggtcagt ggcagtgcca gtcctggatg cagagcgtcg aatccacgac
     3601 cagcgcgtga ggcatgaact tcttgtgggg cgatttctga tatttgtagt atccggccac
     3661 ggcgatcatc acggcgttgt ccgtgcagag cagcggggaa gccatgtgga tcttctgttt
     3721 tttggcgcgg ttcaagaaag cggtccgcaa cgccgagttg gcggccacgc cgcccgtcac
     3781 cacgatgttc ttcagaccca gatgttcggc ggcggccagg gtttttccga ccagaacgtc
     3841 gatcaccgcg gcctgaaaac tggcgcagac gtcggctact tttttcttgg actccttcac
     3901 gccggggttc ttttcgagat ggtagatcac cgcggtcttc aagccggaga aggagaagtc
     3961 ccagccggat ccgaggaacg gccggggaaa ggggatggcc gtggcgcggc ccttggccgc
     4021 caagcggtcg atgatggggc cgccgggata tcccagctcg agggctttcg ccaccttgtc
     4081 gaaggcctcg ccggcggcgt cgtcacgcgt gcggccgagc accacgaact gtccgtagtt
     4141 cttcacgtgc accagttccg tgtgtccgcc cgaggtcacc aacgccagaa acggcggatg
     4201 cagatcgggg ttggccggca aaacggagag cacgtggcct tccaaatggt tcacccccac
     4261 cagcggtttc tggtaggccc aggcgaacat ctcggcggcg atctttccga tgagcaacga
     4321 tcccaccagg ccgggtccca tggtggccgc aatcacgtcc accggcaagg ttttcgggaa
     4381 agggaactgg ccccgcccgc cgaacccctt gagggcctcc tgcaccacgg tctggatgtt
     4441 gcccacatgc gcccggctgg ccagttcggg caccacgccc gagtagggtt gatggatcgc
     4501 cacctgagag gcgatcacgt tcgataaaat ccgccgaccg ccttccacca ccgccgccgc
     4561 ggtctcgtcg caggaggttt ctattcccaa aacgcgcagg tcttttgaat ccgtcatctc
     4621 ggccgcccct ccgggtgatt tgtcgcgcta ttacaaaacg tcattgtagc aattttcgac
     4681 gatgcgggga gcgtgaaaag ccgcggcggg cccggcggcg gaatcaactc tcaaccgccg
     4741 accttggcac cgcgagcggc atttgttttc cgcgcccacg cttttagaaa ctccggctgc
     4801 tttttttcct gggcctccaa caagaatata ttctcctggc agaaacaatg ccgggcggga
     4861 cacggcgccg gcgccggcag aagttcaaag tcgtcgccga agaggttccc cagcctcgcc
     4921 tccgcctcca tgcccgggcc cgttccgccg catcgaaaga cctccccatc cgccttcacg
     4981 ttggcgtaaa accagcccgc gcaacagggc ttccctttca gggagggctg ttccacggga
     5041 gccaccggtt ggccctcccc cgcgggacgg gtcccgggtt ccagatcaag caaagccctt
     5101 tcctcgacgc tgaaactttc cggatagctt ttcccctggt atttcccctg aaaaacctga
     5161 aacgtgatcg ggatcccctc cttggctagg agttcccgga tccgtgtcat ctgcggaatg
     5221 tgcggcggat aggaaaccat ggcggccgac ggagtaaaac cgagacttct caaatacaaa
     5281 agtttcttca agaaaagatc cggcgcggcg aactcggggt ggaaactggc gttgagatag
     5341 atcgccgagg cgtcccaccg ggcggtcatt tttttcaact cttccagact gctggacaga
     5401 ttggtggcga cataaaggta atggtgtttc gaaagttccg caaaaagatc gccgacgccg
     5461 ggataaagga ggggttcccc gccaagcaca tgcagccggc agcgcccgta tttttcattc
     5521 atgcggctcc acggcctcag ccacgcctgc cacgggatgg gatggtcttt tttgtcgaaa
     5581 tattgccact gatctgcctg ctcccaccag caataactgc atcggtaatt gcagaggtta
     5641 tgcaaatccc aggtaaaaga cggcccccca tcaaacgctt ccttccacca cggctggaac
     5701 ggcggttcgc ttttcccttc ggttcccata agcagtcgac ttccctcgtc tttcgtgatc
     5761 ttaaaccgcg ttggctcggg attgcttctc tttttcaacc ggccaaagct cccgtcccca
     5821 tcgatcaaga ccccaacggc gccgatcgtg cacgcatccc cttagtggaa gcgtgcgccg
     5881 atgacaccgt cggaagatag acggaaactc cgaccgacgc cgcaaaaacg gcgcccacgg
     5941 cgaaacgatt tttcgacttt ccaaacgaat ggggatcggg ccggcggctc agatatgaaa
     6001 aaccagtttc tggaaaacga cgtcctcgga ggcgcccagt tccagtatcc atttcttgtc
     6061 ggtcttgtcg ttccgaacaa gccggtagcc tttctttgga aggacggacc cgagctgttt
     6121 caggaaacgg gccgagttga aaccggcggg atagtccatt tccagcgtgt cttccagcca
     6181 acggatcccg tccatttctc tttcctgatt gaaggacttc agcacctggc cgtcctgcag
     6241 gccgttggcc gccaaaagat cgcgggtcgt cctctggagc caggcggaat ctttcacaaa
     6301 atccccggag agcccggcct tgcgggacgg aaggagccgc cgcaggagcg cgg
//
LOCUS       DAOTFD010000150         6350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8768_length_6350_cov_4.302621, whole genome shotgun sequence.
ACCESSION   DAOTFD010000150 DAOTFD010000000
VERSION     DAOTFD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_6350_cov_4.302621"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="P5079_08485"
     CDS             <1..1216
                     /locus_tag="P5079_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30054.1"
                     /translation="DVIGGSESYTFQLANLLSFFADVDILTTTAKDHFSWKNHFPEGI
                     EVVSDHLRIRRFTVDFERTQHWFELNRILLNGVPMLDFAFMEPAVKQNYVSEIRKIPA
                     GFQEEWMRQQGPYSSALLDYLKNHADYDHYLFMTYLYPTTYFGVDQIAGPKKIHIIPT
                     LHDEVPAYLNIMQKYARLKFLFLTRAESRLAQRIFHSEIRFDIIGYGLPDRFASIKPF
                     QSPDPYILYAGRLDPSKGVEKLYEYFKRFVKKHPQLKLYTIGDGPLKEEKHPNIRYMG
                     FVDETQKLALMGGALSFVHPSSFESLGIVLLESFMMGTPGLVNKRCEVLNDHILESGA
                     GFSYDTYEGFENSLMALLDDSTLRDKLGSNARKYYLERYSVETAQSKLAQILGTIGSQ
                     GEVSNNSATARP"
     gene            1213..2304
                     /locus_tag="P5079_08490"
     CDS             1213..2304
                     /locus_tag="P5079_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY30055.1"
                     /translation="MKRTVLINGRFLGQPVTGVQRYAREVLSVMGAMPDNPFHLVLAC
                     PNRPKGESFGNVEILNDASPLPGAMWQQIRLPWIIQKTKADLLWSPCNIGPLFAENHV
                     VTIHDASVFAHPECFSPLFRAYYRAAWKSLGRRARLIITDSNFSKNELIRYRIAKPEK
                     IRVVYAGHREMDASAKPQPIVDGPYVLTVGSRDPRKNLGRLLQAWEKLGTSKKNRRLI
                     VAGGGAASFSSEKIGKVPSDVVFTGHVPDEKLAALYAGADAFLFPSLYEGFGMPPLEA
                     MSSGIPVAVSDIPVLREMCGNAAVYVNPTDPDDIRHGLHEILSDTDLRRRLVEKGRAQ
                     VGSFTWDKTAAGILNTFREILNEGENKRT"
     gene            2301..3320
                     /gene="gmd"
                     /locus_tag="P5079_08495"
     CDS             2301..3320
                     /gene="gmd"
                     /locus_tag="P5079_08495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRY30056.1"
                     /translation="MTKTALITGVNGQDGAYLAQLLLEKGYQVFGAIRSPAEMKLDNL
                     ETLGVMNDVKIIPFELLDYANIFRVVEKVNPDEVYNLAAMSFIAMSFEEPFLTSEVTG
                     MGPLRLLEAIRTVNPKIRFFQASSSSMFDQTQKPPQIESTSFHPKNPYAVAKLFAHWT
                     TVNYRETYGMFACSGILFNHESPLRGLEFVTRKITHAAARIKKGLQQDLRIGNLDVQR
                     DWGYAKEYVDAMWRMLQQPQPEDFVIGTGETHSVREFVEAAFKAVDITITWSGNGEGK
                     IGKNAKTGDALITIDPQFFRPTEVDNLVADYSKARRLLGWSPKTQFNDLVKLMVDHDL
                     KKLPE"
     gene            3329..4891
                     /locus_tag="P5079_08500"
     CDS             3329..4891
                     /locus_tag="P5079_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30057.1"
                     /translation="MLKKLFLFFGRHPEPKAKNPVIFLHAAQEYPSPALRMTLPEVAP
                     HPVKKFWQSQFYAKNFTRIALWGLGLWAILAYLRHYRIEGDTVALVNGVSAMWSCMKQ
                     GHWAGCPGVLHFPLLQQLPALFLTALGLNKNIILRLLVLLNLALFLQMMRSSWRNLAS
                     SSQLLANSFVFVLLAGPLLWYARSSFGEMLATFCTLNLVMSFLTQTSPAHIFIWFLLA
                     GISKETAFPFLLLLSMAAWSLKRGTQPRYFRRHPLAILIPALVVVLTLNTSLNYFRFA
                     SPINTFLLNTPVLRVPNWEIQWSFFAGIWLSPNGGVLFFWFPMFTMLVLCLWTAAKTV
                     FGDSVVSGKKLLTRLAPAIAVLLTLFGLTWGFSHWFAPMGWVALGPRLLLPWLPAVAY
                     LMLSAYPGDVEKVILTLFENRNRAIAVYIAMVFCTLPQFVILFKPSLLDVFFLPDKNC
                     PRIADVMENVDYYYACINHLLWFKKPLFLKCYSVFFGNLPIFLLSAFSAVNILALLPK
                     WGKNTSAREPIS"
     gene            4913..6328
                     /locus_tag="P5079_08505"
     CDS             4913..6328
                     /locus_tag="P5079_08505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30058.1"
                     /translation="MLSVIIPIYNERHTLREILRRVCLVDIPKQIILVDDASTDGTRR
                     TLQTMEKDISDWTNRYPKNKFLFVYQPRNRGKGAAIREGISRATEPFTIIQDADLEYD
                     PNEYPKLLEPLLRGQADVVYGSRFSGTHRRVLFFWHSLGNKFLTFLSNAFSNLNLSDM
                     ETCYKVFRTDIIKGIPLRSERFGFEPEITSKIAKLGCRIFEVPIDYYGRTYAQGKKIG
                     LKDAFQALYVILKYWLIDDLYTTESAGLRTLRIMEGAGKYNDWLFKQCEPYLGNRVLE
                     MGSGIGNITNFLLDKDLVFATDISDSHVKELEREFGHFPNVRVVQLDMSDPATKVGIK
                     DKIDTIISMNVLEHIENDGQALKNCHELLPKGGKLVLLVPAHQSLYCNMDRNIEHKRR
                     YDLKPLQKQLEDVGFKIQMGHHLNVLGAIGWFVNGRLLRRQIVPSRQLRLFDFFVRFL
                     AYEKRFKPSFGLSILIVAEKI"
BASE COUNT         1511 a         1731 c         1503 g         1605 t
ORIGIN      
        1 agatgtgatt ggggggtcag agagctacac ctttcaattg gcgaacctcc tcagtttctt
       61 cgccgacgtt gacatcctga caaccacggc caaggatcat ttttcctgga aaaaccattt
      121 cccggaaggg atcgaggttg tttcggacca tctccgcata cgccgtttca ccgtggattt
      181 cgaaagaacc cagcattggt tcgaattgaa ccgcatcctc ctcaacggcg tgcccatgct
      241 cgatttcgct ttcatggaac ccgccgtcaa acaaaattac gtttcggaga tcaggaaaat
      301 accggccggt ttccaggaag aatggatgcg gcaacaaggc ccttattctt cggccctgct
      361 ggattatctg aaaaaccatg ccgactatga ccattacttg ttcatgacat acctttaccc
      421 cacaacttac ttcggcgttg atcaaattgc cggcccgaaa aaaatacata tcatccccac
      481 actccacgac gaagtcccgg cctatctgaa catcatgcag aaatacgccc ggctcaaatt
      541 ccttttcctc acacgagccg aaagtcggct ggcacagcga atcttccatt ccgaaatccg
      601 cttcgatatc atcggttacg gtctgccgga ccgcttcgct tcaattaagc ccttccaatc
      661 cccggatccc tacatccttt atgccggacg gctggacccc tccaaggggg tggaaaaact
      721 ttatgagtat ttcaaacggt tcgtaaagaa acatccccaa ttaaagctct acaccatcgg
      781 cgacggcccc ctcaaggagg aaaaacatcc caatatccgt tacatgggct ttgttgacga
      841 aacgcaaaaa ctggcgttga tggggggggc attgtcattc gttcacccct cgtcatttga
      901 gagcttggga atcgtccttc tcgaatcgtt tatgatggga acgccagggt tagtgaacaa
      961 acgctgcgag gtgttgaacg accacatcct cgaatccggt gccggattct cctacgatac
     1021 gtacgaggga tttgaaaatt cgctgatggc gttgctcgat gattcgacgc ttcgtgataa
     1081 gctaggcagc aatgcgcgca aatactacct ggagcgctat tcggttgaaa cagcccaatc
     1141 gaaactagcg cagattttgg gaaccatcgg cagtcaaggg gaagtttcga acaattcagc
     1201 aacagcccgc ccatgaagag aacagtcctt attaacggcc gattcctcgg gcaacctgtt
     1261 accggcgttc agcgttacgc gcgagaggta ttgtccgtca tgggggcaat gccggacaat
     1321 cctttccacc ttgttttggc atgtcccaat cgtccgaagg gggaatcttt tggcaatgtc
     1381 gaaattctta acgatgcttc cccgttgccc ggagcaatgt ggcaacaaat ccgccttccc
     1441 tggattattc aaaagacaaa ggcggacttg ctgtggagcc cctgcaatat agggcctctt
     1501 ttcgccgaaa accatgtcgt caccatccat gacgcatcgg tgttcgctca tcccgaatgc
     1561 ttctccccgc tgttccgcgc ctactaccgg gccgcctgga aatcattggg caggagggcg
     1621 cgtcttatca tcacggattc gaatttttca aagaatgaat tgatccgcta tcggattgcg
     1681 aaacccgaaa aaatacgtgt agtatatgcc ggacaccggg aaatggatgc ttccgccaag
     1741 ccccaaccga ttgttgatgg tccgtatgta ttgaccgtcg gctcacggga tcccagaaaa
     1801 aacctgggcc gcctcctgca ggcatgggaa aaactgggaa cttctaagaa aaacagaaga
     1861 ctaatcgtgg ctggtggggg agcggcgagc ttttcctcgg aaaaaatcgg gaaggtccca
     1921 tccgatgtgg tttttaccgg ccatgttcct gatgagaaac tggccgcttt gtacgcgggg
     1981 gcggatgctt ttctgttccc gtccctctat gagggcttcg gcatgccgcc gttggaggcc
     2041 atgtcttctg gcattccggt tgccgtctcc gacatccccg ttttgcgcga aatgtgtggg
     2101 aacgccgccg tttatgtcaa tccgacggat cctgacgaca tccgccacgg cctccacgaa
     2161 attttgtcgg ataccgattt aagacggcga ctcgtcgaaa aaggccgcgc gcaggtcggc
     2221 tcttttacgt gggataagac tgccgccggc atactgaata cttttagaga aattttaaat
     2281 gagggggaaa acaaacgaac atgacgaaaa ccgctctgat cactggggtg aacggccaag
     2341 acggcgccta tttggcgcag ctattgttgg agaagggcta tcaagtcttc ggcgccatcc
     2401 gaagcccggc ggaaatgaaa ctcgacaacc tggaaacgtt gggggtaatg aacgacgtca
     2461 aaatcatccc tttcgagctt ctggattatg ccaacatatt ccgggtggtg gaaaaagtga
     2521 acccggacga ggtctacaac ctggccgcca tgagcttcat cgccatgtcg ttcgaagaac
     2581 cgttcctcac gtcggaagtc accggcatgg ggccgttgcg tcttttggaa gccatccgaa
     2641 cggtcaatcc caaaatcagg ttttttcagg cctcttcaag ctccatgttc gaccaaaccc
     2701 aaaaaccgcc tcagattgaa tcaacctcgt tccatcccaa aaatccctat gccgtggcca
     2761 agctctttgc ccattggact accgtaaatt atcgagaaac gtacgggatg ttcgcctgtt
     2821 cgggaatact gttcaaccac gagtctccgt tacgcggctt agaattcgtc acacgaaaaa
     2881 tcacccatgc cgccgcgcga atcaaaaaag gacttcaaca ggatttgcga ataggcaatc
     2941 tggacgtcca gcgcgattgg ggctacgcca aggaatacgt ggacgccatg tggcgtatgc
     3001 tgcaacaacc gcaaccggaa gattttgtca tcggtaccgg cgaaacccat tccgtccggg
     3061 aattcgtgga ggcggctttt aaagcggtgg atataacgat tacctggtca ggaaacggcg
     3121 aggggaaaat cgggaaaaac gccaaaaccg gagacgcttt gataaccata gaccctcagt
     3181 ttttccgccc cacggaagtg gataacctcg tggcggatta ttccaaagcc cgtcgtctgt
     3241 tgggctggtc gccgaaaacc cagttcaacg atctcgtcaa actcatggtg gaccacgacc
     3301 tgaaaaaact gcccgaatga ttaatagaat gctgaaaaaa ttatttttgt ttttcgggcg
     3361 tcatcccgaa ccaaaggcga aaaatcccgt catttttctt catgcagcac aggaatatcc
     3421 ttcgccggca ctccggatga cactaccgga ggttgccccg catccagtca aaaagttctg
     3481 gcaatctcaa ttctacgcga aaaacttcac ccgaatcgcc ctatggggac tcggcctgtg
     3541 ggcaatcctc gcctacctcc ggcactatcg aatcgaggga gacacagttg ccctggttaa
     3601 tggtgtatcc gccatgtgga gctgtatgaa acagggccat tgggccgggt gtccgggagt
     3661 cctccatttc ccattgcttc aacagttacc ggctctcttc ctcacggcgc ttggcctgaa
     3721 caaaaacatc atcttgcgat tgcttgtatt attgaatctg gcactcttcc ttcaaatgat
     3781 gcgatcctcg tggagaaacc tggcttcatc ttcgcaactc cttgcgaatt cctttgtttt
     3841 tgttctcctc gccggtcccc tcttgtggta tgcgcgctct tctttcggtg aaatgctggc
     3901 gacgttttgc accctgaatc ttgtcatgtc tttcctaaca caaacaagtc ccgcccacat
     3961 tttcatctgg ttccttctgg ccggcatctc caaagaaacc gccttcccgt tcctccttct
     4021 cctgagcatg gcagcgtggt ctctgaagcg gggaactcaa ccacgttatt tcagacgcca
     4081 tcccttggct atccttatcc cggccctcgt cgttgtttta actctcaata cgagcttgaa
     4141 ttatttccgc ttcgcctctc ccatcaatac gtttcttctg aacactcccg tgctgagagt
     4201 cccgaactgg gaaatccagt ggagcttctt cgcggggata tggctttctc ctaatggagg
     4261 cgtgttgttc ttttggtttc ctatgttcac catgctcgtc ttgtgcctct ggacggcggc
     4321 aaaaacggtg tttggtgatt ccgttgtctc cggcaaaaaa cttctcaccc ggcttgctcc
     4381 ggcaattgcc gtgttactca cgcttttcgg tctcacctgg ggattctccc actggttcgc
     4441 tcctatggga tgggtggcgt tgggtccccg tcttctgctc ccctggttgc cggcggtcgc
     4501 ttatttgatg ctctccgcct atccgggcga tgtggaaaag gtcatcctta cgctttttga
     4561 aaataggaac cgggccatcg ccgtttatat cgccatggtc ttttgcacct tgcctcaatt
     4621 tgttattctt tttaagccgt ctttgcttga tgttttcttc ctcccggaca aaaactgtcc
     4681 gaggattgcg gacgtcatgg aaaacgtcga ttactattat gcgtgcataa accatctgct
     4741 ttggttcaaa aaacctcttt ttttgaaatg ttactcagtc tttttcggca acctgcctat
     4801 ttttttgttg tccgctttta gtgccgtgaa cattctcgct ctgctcccga aatggggaaa
     4861 aaatacatcg gcaagagaac cgatttcata aagagaaaag ggccaatgcg ccatgttgtc
     4921 cgtcattatc cccatatata acgaacgcca cactcttcga gaaatcctcc gaagagtctg
     4981 cctggtggat attcccaaac agatcatcct cgtggacgac gcttccaccg atggcacgcg
     5041 ccgaaccttg caaaccatgg agaaggacat ttctgattgg acaaaccgtt acccgaaaaa
     5101 caaatttttg tttgtctatc agccccgcaa ccgcggcaag ggcgcggcca tccgcgaagg
     5161 gatatcccgg gccacggagc ctttcaccat catccaagac gccgacctcg aatacgatcc
     5221 taacgaatat ccgaaacttt tggagcccct gcttcgcggc caagccgacg tcgtctacgg
     5281 atcacgcttc tccggcactc accgtcgcgt gctgttcttt tggcatagcc tgggcaataa
     5341 attcctcacc ttcctctcaa acgctttctc taacctgaac ctatccgata tggaaacttg
     5401 ttacaaggtc ttccgtacgg acattatcaa gggcatcccg ctccgctcag aacgtttcgg
     5461 attcgagccg gaaattactt ctaaaattgc caaactgggc tgccggatct ttgaggtgcc
     5521 tattgactat tacggtcgca cctatgctca aggtaaaaaa atcggactaa aggacgcttt
     5581 tcaggctttg tatgtgatcc ttaaatattg gctcatcgac gatctttaca ccaccgagtc
     5641 cgcgggactg cggacattgc gcatcatgga gggggcgggg aaatataacg attggctttt
     5701 caaacagtgc gagccttacc tgggaaaccg tgtattggag atgggctccg gtatcggcaa
     5761 catcaccaat tttttgctgg ataaggacct cgtgtttgcc actgatattt ctgattccca
     5821 tgtaaaggaa ttggaacgtg aattcgggca ttttccgaac gtgcgtgttg tgcagttgga
     5881 catgagtgat cccgccacca aggtgggaat caaagacaaa atagacacga tcatttcaat
     5941 gaacgtcctt gaacacattg aaaacgacgg gcaggcactg aaaaactgcc acgagcttct
     6001 gccaaaaggg ggaaaactgg tcttgcttgt ccccgcccac caatcccttt attgcaatat
     6061 ggaccgaaac atcgagcaca aacggcgtta cgacctgaaa ccgctccaaa aacagcttga
     6121 agacgtcggc tttaaaattc agatggggca tcatctcaat gtcctggggg ctatcggctg
     6181 gttcgtcaac ggccgtcttc tccgtcgaca aatcgttccc agtcgtcaac tacgtctttt
     6241 tgattttttc gttcgcttcc ttgcctatga aaaacgtttc aagccgtcct ttggcctgtc
     6301 tatattaatc gttgccgaga agatataatt taatcaaacc gcttccttgt
//
LOCUS       DAOTFD010000151         6342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8781_length_6342_cov_4.145061, whole genome shotgun sequence.
ACCESSION   DAOTFD010000151 DAOTFD010000000
VERSION     DAOTFD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_6342_cov_4.145061"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..869
                     /locus_tag="P5079_08510"
     CDS             243..869
                     /locus_tag="P5079_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRY30059.1"
                     /translation="MWLDYFRAFSNACSLAAAFISGLDPAIRKQVVDNLAAPQMLMRD
                     EVKALGQSLRQRVRGVIYGIDQYFAIYDSATPAAQTELLKALESQTPALVEKMRNEMF
                     TFDDLLALDSGALRVVFREVPLRTLATALMGAAVSTRDRVLTVLPSGAAEIIRQEIEM
                     NPVHSQKTIEDERKKIVQVVRRLIWDKKIVMPPRQKGGSRTPPQPARV"
     gene            885..1664
                     /locus_tag="P5079_08515"
     CDS             885..1664
                     /locus_tag="P5079_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY30060.1"
                     /translation="MDLMTIIGLISGAACVYYVMWQSGIAALLINTSAFILVFGGAFS
                     CILITYPWAIIRRLHHALRMVFVPPKMETASQIIQRLVRLAEQAKRGGVDTLEQELAG
                     LQDRFLSDGLRMVVNGLPPELIRENLEKEIAFTRVRHQQLANVFKTLGTYAPVFGLLG
                     TLIGVVRILQNLTDPQSLGSAMAIAVTTTFYGIFGTNFIFLPIAGKLNAHSDAEMLVK
                     EVMIEGILSVQAKEVPAIVSLKLQAYLSHRFRDAGLKPPSK"
     gene            1687..2406
                     /locus_tag="P5079_08520"
     CDS             1687..2406
                     /locus_tag="P5079_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRY30061.1"
                     /translation="MSGNASSRHKSADRIRADFAEGGVDTVNVWQTVYGDMMTNLMLF
                     FLMMFSLNMVGKEQFEKSAQAFKNTFSDNEPTLAKPAGDRPAKEVKEVKDLNQYLSKF
                     VKEEENVEVIEHGEGVRLRLPEPVLFDLGSAELKDEAKRVLGEIAKGLKNLPNRVVVE
                     GHTDDIPIGRGPYKSNWELSEARAENVANYLIYEEGVPPERVAVAGFGEFWPFVVNDS
                     DLNRALNRRIELLVIADEKEI"
     gene            2390..3001
                     /locus_tag="P5079_08525"
     CDS             2390..3001
                     /locus_tag="P5079_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRY30062.1"
                     /translation="MKKKFSLQADGTGEATQNIWVTTYGDMMTNLMILFMVMWTYTQF
                     LHKDIANPAMPMPNATKQQILRVELKKELQAVGTVLMTKRKVIVTLPSAVLFDPGSEE
                     LKATALEPLDRVADALAKSTAPIVVEGHTDDIPITHSHWRSNYELSAARAFSVIRYFT
                     EVKGLPPSRLAARGYGPYRPTVANDSDESRARNRRIEIHLWTS"
     gene            2998..4221
                     /locus_tag="P5079_08530"
     CDS             2998..4221
                     /locus_tag="P5079_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY30063.1"
                     /translation="MIPPKLFSTGLCLLFLSLAVSGLAPAAVEGKDPPALWDAADPSS
                     PAKTPSSQTADLKARLTAAQEEGDAALTFVLADLLSSLEPEQFRWENVRNEAGERLAG
                     RTAVPDLEPLFKKRLREAAKELQSAELMTEEAGSEAELEKEVAQFQKTAAQLKREADA
                     LAVKNTLLAEEEDGLIRQMQTEPSDELADRLKRVTQERKRNETERLAIDEKTARLEKR
                     LAESRDLLTFSRQRDSETGGRLLQTQTAYRTTKKDSAYFGLWKDMKTVSTKNKKAAAK
                     GKPPEAPPDTEDFSSETQGPPAPLDEVRPSQKKEMRPPAPPKPAAPAPKKPAVPAAPP
                     APPFLPAPSALPQPAVVHPVQEGETLPSLAQKYYGDPARWREIYEANAHAILRGVLEP
                     GQRLLIPQGEKRQTP"
     gene            4306..6159
                     /locus_tag="P5079_08535"
     CDS             4306..6159
                     /locus_tag="P5079_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30064.1"
                     /translation="MDQINKLNEQINDLWEKMRKPSASSLPPSASADFLSAERDLLRQ
                     TIAHLRRHFDEEKDRWQKLLAERDEAVQGLKEEKRWLDEQAARQRQELAALHSNLQSQ
                     LEVAAFEKTELDAELVRRIRQHEDELRLYQQQQQNLEAGHRQEVDRLQAALNRWKEEE
                     QTWHAMVEQKNQALAGLSQRINELELQRQKDRHEWDEGKRQLEKTHRDTAAQTEKEKT
                     ELKETLEVRDAEIENYKRLLQMSQEEIVDFTRVRHKLETELRAADEQIGRLQNEIANM
                     REAWEVERGHWRELWERERASREKWYDNMREWEDHLRREREDWLTQFRTEQSSREATA
                     KRMDAAVDRLGDTTARLVAPPPVTRAAASRRFAVSPVRVWGTAAVFVGLTALAVLLLR
                     ACGAPKEFNLPSPNISGLTVYGSDLWMSDWMNGRLLQASADRPDKATALGKPSATFHP
                     VSLRRFEGSLWSLDPWTRTLQEHALLPPFVARRAWPVLASTPVDFCWDGQGVWILDGG
                     AQILRRFPRGDFSVPDREVPLPADWKIIALDYADGAFWGYDKAAARLRRFVLSPTVSV
                     SAEYRLPVHQQPGSPLTALSVHFNELWTASEKSQTLTRWSRWALRRHKIRS"
BASE COUNT         1291 a         2171 c         1864 g         1016 t
ORIGIN      
        1 cgccagctga tgaccccacc ggaagcgccg cggcgacaga ttggcttcca cgtcagggat
       61 taacgccgtt cgtgatgtcc ccgcggccgt cggcggacgc gcgggccttc gccacgccga
      121 cttgccgccg cgacggagcg ctctggcgta gtcaggagtg gcttcggcta cgcaggcggg
      181 agcgcgtccc tccgcaaaca aaaaaagcgg gagccccgct gccggggctc ccgctggagt
      241 tgttgtggct ggattacttc cgagccttct cgaacgcctg ctccctggcc gccgccttca
      301 tctccggcct cgaccccgcc atccgcaaac aggtggtgga caacctcgcc gccccgcaga
      361 tgctgatgcg cgacgaggtg aaggcgctgg ggcagagtct ccgtcaacgc gtccgcggcg
      421 tcatctacgg catcgaccag tatttcgcca tctacgactc cgccacgccg gccgcccaga
      481 cggaactgct caaagcgctg gagtcccaga ccccggccct ggtggaaaag atgcgcaacg
      541 agatgttcac gttcgacgat ctcctggcgc tggattccgg cgccctccgc gtggtgttcc
      601 gcgaggtgcc tctccgcacg ctggccaccg cgctcatggg cgcggcggtg agcacccgcg
      661 accgcgtgct caccgttctg ccttccggcg ccgccgagat catccgccag gaaatcgaaa
      721 tgaacccggt gcacagccag aagaccatcg aggacgaacg caagaagatc gtccaggtgg
      781 tccgccgcct catctgggac aaaaagatcg tcatgccgcc gcggcaaaaa ggcggctcgc
      841 ggaccccgcc ccagccggcg cgggtctaaa ggggaaacca cgacatggac ttgatgacca
      901 tcatcggcct tatttccggt gccgcctgcg tttattacgt catgtggcag tccggcatcg
      961 ccgctttact catcaatacc agcgccttca tccttgtctt cggcggcgcg ttcagttgca
     1021 tcctcatcac ctacccctgg gccatcatcc gccggctcca tcacgcgctc cgaatggttt
     1081 ttgttccgcc gaagatggag acggccagcc agatcatcca gcggctcgtc cgcctggccg
     1141 aacaggccaa gcggggcggg gtggacaccc tggaacagga actggccggg ttgcaggacc
     1201 ggttcttgtc cgacgggctc cgtatggtgg tgaacgggct gccgccggaa ctcatccgcg
     1261 aaaacctcga gaaggaaatc gccttcaccc gggtgcgcca ccaacaactg gccaatgtct
     1321 tcaagaccct cggcacctac gccccggtct tcggattgct gggaaccctc atcggcgtgg
     1381 tccgcatcct gcagaacctt acggaccccc aatcgctcgg ttccgccatg gccatcgccg
     1441 tcaccacgac cttctacggc atcttcggca ccaacttcat cttccttccc atcgccggaa
     1501 aattgaacgc ccactccgac gccgagatgc tggtgaagga ggtcatgatc gagggcatcc
     1561 tgtcggtgca ggccaaggaa gtgcccgcca tcgtcagcct gaagctccag gcctacctct
     1621 cccatcggtt ccgggacgcc ggcctcaaac ctccctcaaa atagccgctg atttttcttc
     1681 ccatccatga gcggcaacgc ttcttcccgt cataaatcgg ccgaccgcat ccgggccgat
     1741 ttcgccgagg gcggcgtgga caccgtcaac gtctggcaga ccgtgtacgg cgacatgatg
     1801 accaacctca tgctcttctt cctcatgatg ttctccctca acatggtcgg taaggaacag
     1861 tttgaaaaat ccgcccaggc cttcaagaac actttttcgg acaatgagcc gactttggca
     1921 aaaccggccg gcgacaggcc ggccaaggaa gtaaaggaag tgaaagacct gaaccaatat
     1981 ttgtccaagt tcgttaagga agaagagaac gtggaggtga tcgagcatgg agaaggcgtc
     2041 cgcctgcgcc tgcccgaacc ggtcctgttc gatctgggga gcgcggaact gaaggacgag
     2101 gccaagcggg tcctcggcga aatcgccaag ggattgaaaa acctgcccaa ccgggtcgtc
     2161 gtcgaaggcc acaccgacga catccccatc ggccgcgggc cgtacaagtc caactgggag
     2221 ctctcggagg cccgcgccga aaacgtggcc aattacctca tctacgaaga gggcgtcccg
     2281 ccggaacggg tcgcggtggc ggggttcggc gagttttggc cgttcgtcgt caacgattcc
     2341 gacctgaacc gcgccctgaa ccgccgcatc gaactcctcg tgatcgccga tgaaaaagaa
     2401 atttagcctc caggccgacg gcaccggcga agccacccag aacatctggg tgaccaccta
     2461 cggcgacatg atgaccaacc ttatgatcct cttcatggtc atgtggacct acactcagtt
     2521 tttgcacaag gacatcgcca accccgccat gcccatgccg aacgccacca aacagcagat
     2581 cctgcgcgtg gaactgaaga aagaactcca ggccgtgggc accgtgctca tgaccaaacg
     2641 caaagtcatc gtcaccctgc cctccgccgt gctgttcgac ccgggaagcg aagaattgaa
     2701 agccaccgcc ctggaacccc tcgaccgcgt ggccgacgcc ctcgccaaat ccaccgcgcc
     2761 catcgtggtg gagggccaca ccgacgacat tcccatcacc cattcccatt ggcgcagcaa
     2821 ctacgagctc tccgccgccc gggcgttttc cgtcatccgc tacttcacgg aggtcaaagg
     2881 cctcccgcca tcccgcctgg ccgcccgggg ctacggcccc taccggccca ccgtggccaa
     2941 cgattccgac gagagccgcg cccgcaaccg ccgcatcgaa attcatctct ggacatcgtg
     3001 attccgccga aacttttttc caccggcctt tgcctgcttt ttctgtcttt ggcggtctcc
     3061 ggcctggccc cggccgccgt cgaggggaag gaccccccgg ccctgtggga cgccgcggat
     3121 ccctcctctc cggccaagac cccttcgtcg caaacggcgg atttgaaggc gcggctgacc
     3181 gccgcccaag aggaggggga cgccgccctg acgttcgtcc tggccgacct gttgtcgtcc
     3241 ctggaaccgg aacaattccg ttgggaaaac gttcggaacg aggccggcga acggctggcc
     3301 ggccgcaccg ccgttccgga cctcgaaccc ctctttaaaa aacgcctgcg cgaggccgcc
     3361 aaggaactcc aatccgcgga actgatgacg gaagaggcgg ggagcgaggc ggaactggaa
     3421 aaagaggtcg cccagttcca aaagaccgcc gcccagttga aacgggaagc ggacgcgctc
     3481 gccgtcaaaa acaccctgct ggccgaggaa gaagacggtt tgatccgcca gatgcagacc
     3541 gagccgtccg acgagctcgc cgaccggctg aaacgcgtca cgcaggaacg gaaacggaac
     3601 gagacggaac gccttgcgat cgacgagaaa accgcccggc tggagaaacg gctggcggaa
     3661 tcccgcgacc tcctgacctt ctcccgccag cgggactcgg aaacgggagg acgcctcctt
     3721 cagacccaga cggcctatcg cacgacgaaa aaagattccg cctatttcgg cctctggaaa
     3781 gacatgaaaa cggtttccac gaaaaacaag aaggccgccg caaaagggaa accgccggaa
     3841 gcccctccgg acacggaaga cttttcatcg gagacgcaag gtcctccggc ccctctcgac
     3901 gaggtccggc cgtcccagaa aaaagaaatg cgcccgcccg ccccaccgaa acccgcggcg
     3961 ccggccccga agaagccggc cgttcccgcg gccccgcccg caccgccgtt ccttccggcg
     4021 ccctccgctc tccctcagcc cgccgtcgtg caccctgttc aggaggggga gaccctgccg
     4081 tccctcgccc agaagtatta cggagacccc gcccggtggc gggaaattta cgaagccaac
     4141 gcccatgcca tcctgcgcgg cgtgctggaa cccggccaac gcctcctcat cccccaggga
     4201 gaaaaacgcc agaccccttg acattcgtct gatttcgcgt aaactttata cagacgccga
     4261 gacgtcggac ccgagagggc cccgcgtgtc ggcgttcttg attttatgga ccagatcaac
     4321 aagctgaacg aacagatcaa cgacctgtgg gagaagatgc ggaaaccgtc cgcgtcgtct
     4381 ctcccgccct ccgcctccgc cgacttcttg tcggcggaac gggacctcct gcgtcagacc
     4441 atcgcccatc tgcgacgcca tttcgacgag gaaaaagacc gctggcagaa gctccttgcc
     4501 gagcgggacg aggccgtgca gggcctcaag gaggaaaaac gctggctgga cgaacaggcc
     4561 gcccggcagc gtcaggaatt ggccgcgttg cattccaacc tgcagtccca gctggaagtc
     4621 gccgctttcg agaagaccga gctggacgcc gaactcgtcc gccgcatccg ccaacacgaa
     4681 gacgaactcc ggctctatca acagcagcaa cagaacctgg aggccggcca ccggcaggaa
     4741 gtggaccgtc tgcaggccgc cctcaaccgc tggaaagagg aagaacagac ctggcatgcg
     4801 atggtcgagc agaagaacca ggcgctggcc ggcctctccc agaggatcaa cgagctggaa
     4861 ctccagcggc agaaggaccg gcacgagtgg gacgagggca aacgccagct ggagaagacc
     4921 catcgcgaca ccgccgccca gacggagaag gaaaagaccg aactgaagga gacgctggag
     4981 gtccgggacg cggagatcga aaattataag cgcctcctgc agatgtccca ggaggaaatc
     5041 gtcgacttca cccgcgtccg gcacaaactg gagaccgaac tgcgcgcggc ggacgaacag
     5101 atcggacgcc ttcagaacga gatcgccaac atgcgcgagg cctgggaggt ggaacgcggg
     5161 cattggcggg agctttggga gagggagcgc gcctcgcgcg agaagtggta cgacaacatg
     5221 cgcgaatggg aagaccatct ccgccgggag cgcgaagact ggctgacgca gttccgcacg
     5281 gaacagtcct cgcgcgaagc caccgccaaa cgcatggacg ccgccgtgga ccggctgggg
     5341 gacaccaccg cccggctcgt cgcgccgcct ccggtgaccc gggccgccgc ctcccgccgt
     5401 tttgccgtga gcccggtccg ggtttgggga acggccgccg tgttcgtcgg cctaacggcc
     5461 ctggccgtcc tgctcctgcg cgcctgcggc gcgccgaaag aattcaacct cccctccccg
     5521 aacatttccg ggctcaccgt gtatgggagc gacctctgga tgtccgactg gatgaacggc
     5581 cgcctcctcc aggcttcggc cgaccggccg gacaaggcca cggccctggg gaaaccgtcg
     5641 gcgacgttcc accccgtgtc cctccgccgg ttcgagggaa gtctctggag cctggacccg
     5701 tggacgcgga cgctccagga acacgctctc ctgccgcctt ttgtcgcgcg gcgggcgtgg
     5761 ccggtcctcg cttcgacgcc cgtcgacttt tgttgggacg ggcagggcgt ctggatattg
     5821 gacggcgggg cgcagatatt gaggcggttc ccccgcggcg atttctccgt gcccgaccgg
     5881 gaggtcccgc tgcccgccga ttggaagata atcgctttgg actacgccga cggcgctttt
     5941 tgggggtacg acaaggccgc cgcccggctg cgacgttttg tcctttcgcc gacggtctcc
     6001 gtgtcggccg aataccggct gcccgtccat caacagcccg gttctccgtt gaccgccctc
     6061 agcgtccatt tcaacgagct gtggaccgcc agcgagaagt cccagaccct cacccgctgg
     6121 tcgcgctggg ccctccgccg tcacaaaatc cgctcctaaa aaaagccggc ccccctcttc
     6181 cttgacacgc cccgccgggt gtgttaaagt ataatttctc cgtcgggaaa caccccgggg
     6241 agaaaaatcc gtcgttgagc gaggaaaccc atggaaatgg aagtcacaga cgctaatttc
     6301 aatacggatg tcctgcaggc caaaacgcct gtgctggtgg at
//
LOCUS       DAOTFD010000152         6272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8929_length_6272_cov_3.933248, whole genome shotgun sequence.
ACCESSION   DAOTFD010000152 DAOTFD010000000
VERSION     DAOTFD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8929_length_6272_cov_3.933248"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="P5079_08540"
     CDS             complement(<1..1033)
                     /locus_tag="P5079_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HRY30065.1"
                     /translation="MSVSFLQPVFLWFLPLALAPLLLHWLTRSKPRPLAFSSLELLRE
                     SVRAHLARSRLMNFLLLLCRCLLLAALCLLFSRPVVHWGKIVDVDDAMTTVFLLDASA
                     SMGASHAGATSFDNAKACIRTFLENAGKDDRFGLVVFSETVEMSVLPTANKDRVAETA
                     SGAELSFRPTDVLPAFESAYQMLAAEREGPQAIVLLSDLAKNGWRRFLSQKAGGREFD
                     PRVRVLLAEMNPEGANAAVVSAAVRSGPQGGLIQGSFEAREWGASPGERRWGLSLNGA
                     TVTQGVLVSAEGGAPPQKFSLPERFLIAGGEAEIRLDPDALPADDRLFFRLERPAGFS
                     VLVVNGSPDV"
     gene            complement(1043..1954)
                     /locus_tag="P5079_08545"
     CDS             complement(1043..1954)
                     /locus_tag="P5079_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY30066.1"
                     /translation="MSEAPVPRPPLLDPLLVAKLATMELRARYVVEGALAGRHRSVRQ
                     GHSLEFTGHRSYTPSDEWRHIDWRVFAKTDRWVVREQQEETNLRAVVLMDVSRSMLFA
                     GPRRLPKIQYAALLSAALAYLLVHRRESVGLGFFNRGLMSFVPPRGGAEHLSRLFGLL
                     EAVSGDGGTDLGASLEEAGQRLPRRCLVVLVSDFLEPPESVLSAVRTLVTRKHEVVAL
                     QVLDPAERDLPFEGDFVFEDLETGAVLRANADELRGHYRRKMEERLQLTARAFHSAAV
                     DAHLFWTDAPLESGLSLFLQKRLARRT"
     gene            complement(1968..2966)
                     /locus_tag="P5079_08550"
     CDS             complement(1968..2966)
                     /locus_tag="P5079_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY30067.1"
                     /translation="MDKSAAQEKDSVLVDRLIEAYGKVRGQLSKIIVGQDAVIEQLMV
                     ALFARGHCVVVGVPGLAKTLLISSLARILDLQFRRIQFTPDLMPSDITGTDVLQEDPI
                     TRERTFRFAPGPVFANIVLADEINRTPPKTQSALLQAMQEYQVTVMGKTHPLPLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFLFQINIDYPSAEEEKEIVLQTTGGYAAELQKVLDA
                     EAILRLQEVIRKVPVAENVVEKTVALVRRTRPQSGAEEFVKDWISWGAGPRASQALIL
                     GAKARCILNGRFAVSWDDVLALCEPVLRHRLILSFRAEAEGVRVRDVIQRLLKNPS"
     gene            3755..5107
                     /locus_tag="P5079_08555"
     CDS             3755..5107
                     /locus_tag="P5079_08555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRY30068.1"
                     /translation="MRPSSTKVVLCGGVRTPIGHLAKSLAGFLPEDLMEIVIRGILQK
                     SSLYPHAVDGVLVGWVGQGSHAPNIARVALLKAGLPEKAHAFTIQANCVSSLESVASA
                     YRHIMMSEGDLYLAGGTESMSTFPYAIRGPRSHKSLRSLDAVKTNWAELLNTPDVCLT
                     DTTEEGLTDPVKKISMAATAEICAQMYSISREEQDAYAAETFRRALAAEKNGFYDTHT
                     VPVKKDGRTVLDRDEYPFLREGLVEKPQMFAKAPALFESGGFTMKDFYANFGEHILGK
                     TFEPGKSQGTVTLFNACARSDGAAGLVVASEERAKQLGLEILAEIKSWGFWGNNPAHM
                     GVSPVFSSAVALERAGLKFADLDHVELHEAFAATSLSIFKVGKEKHGQDWRKLWDSGR
                     LNPNGGSIPLGHPLAATGARLLLNLLYAMKQDPGARFGLVAACAAGGLGGAMVVEKYR
                     "
     gene            complement(5141..5308)
                     /locus_tag="P5079_08560"
     CDS             complement(5141..5308)
                     /locus_tag="P5079_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRY30069.1"
                     /translation="MALKKELLEILACPKCKGDLEYRESENRLLCQKCKLAYAIKDGI
                     PVMLIDEAKPI"
     gene            complement(5456..>6272)
                     /locus_tag="P5079_08565"
     CDS             complement(5456..>6272)
                     /locus_tag="P5079_08565"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRY30070.1"
                     /translation="NTLAAYGADLRRFLEHLHRHKKNPLRIPHAEITDYLWQRRQTLK
                     SASLFRLAESIKQFYHYLLAEELISEDPTARMSSPKLTHRLPRYLSEEEVGRLLNRVP
                     DGRESSVRFKAMLELMYAAGLRVSELVNLEEAQLDLDVGFVRAFGKGGKERIVPIHRR
                     AVQSVGAYLDVKRKKFPNAARYVFLSNRGKPLSRVAFWYQLRKWARDCGLKKPLHPHM
                     LRHSFATHLLGGGADLRSVQEMLGHADISTTQIYTHVEKDQLKRMHKQFHPRG"
BASE COUNT         1265 a         1997 c         1904 g         1106 t
ORIGIN      
        1 ccacgtccgg cgaaccgttg accaccaaca ccgaaaaccc cgccggccgc tccaagcgga
       61 aaaacaggcg gtcgtccgcc ggcaaggcgt ccggatccaa ccggatttcc gcctctcctc
      121 cggcgatcaa gaaccgttcg ggcagggaaa acttctgcgg aggagcaccg ccttccgccg
      181 acacaaggac cccctgggtc acggtcgctc cgttcagaga aaggccccac cgccgttccc
      241 cgggagacgc cccccactca cgcgcctcga aactcccctg gatcagtcct ccttgcggtc
      301 ccgaacgcac cgccgccgaa acaacggccg cgttggcgcc ctcgggattc atctccgcca
      361 ggagcacccg caccctcgga tcgaattccc ggcccccggc cttctgcgac agaaaccgcc
      421 gccaaccgtt tttcgccaag tcggacagga gcacaatggc ctgcggcccc tccctttcag
      481 cggccaacat ttggtaagcc gattcaaaag ccggcagcac gtcggtggga cgaaaagaca
      541 gctccgcgcc ggacgcggtt tcggcgacgc ggtccttgtt cgccgtcggg aggacggaca
      601 tctccacggt ttcggaaaac accaccagcc cgaaccggtc gtctttcccg gcattctcca
      661 aaaaggtccg gatgcacgct ttggcgttat caaaggaggt cgccccggca tggctcgcgc
      721 ccatggaggc ggaagcgtcc agcaagaaaa ccgtggtcat ggcgtcgtcg acgtccacga
      781 tcttccccca atggaccacg ggacgcgaga acaacaagca cagcgccgcc agcaggagac
      841 agcggcagag gaggagcagg aaattcatca ggcgggaccg cgccaagtga gcgcgcacgg
      901 attcccgcag aagctcgagg gagctgaacg ccaacgggcg cggttttgaa cgcgtgagcc
      961 aatggaggag gaggggcgcc aacgccaggg gcaggaacca aaggaaaacc ggttgcagga
     1021 aagaaacgct cataggcgtt cttcaggtcc gccgggccag tcttttctgc aagaaaaggg
     1081 agaggccgga ttccaacgga gcgtccgtcc aaaaaaggtg cgcatccacg gcggcggaat
     1141 ggaaggcgcg cgcagtcaac tgcagccgtt cctccatctt gcggcgataa tgcccgcgca
     1201 gttcgtcggc gttggcccgc aacacggccc ccgtctccaa atcctcgaaa acgaaatccc
     1261 cctcgaacgg gaggtcccgc tccgccgggt ccaggacctg cagggcgacg acctcatgtt
     1321 tccgggtcac gagcgtccgc accgccgaca acacggattc cggcggttcc agaaaatccg
     1381 acaccagcac caccaggcac cgccggggca atcgctggcc cgcctcctcc aaagaggcgc
     1441 ccaggtccgt ccccccatcg ccggagaccg cctccaaaag cccgaacaaa cgggacagat
     1501 gctccgcccc gccgcggggc ggcacgaacg acatcagtcc gcggttgaag aacccgagtc
     1561 ccacggattc ccggcgatgc accagcaggt aggcgagcgc ggcggaaagg agggcggcgt
     1621 actggatttt cggcaggcgc cgagggccgg cgaacagcat ggaacgcgac acgtccatca
     1681 ataccacggc ccggaggttc gtctcttcct gttgttcacg aacgacccag cggtcggtct
     1741 tggcgaacac gcgccagtcg atatggcgcc attcgtcgct gggggtatag gagcggtggc
     1801 cggtgaattc gagggaatgt ccctggcgga cactgcgatg gcggccggcg agggcgcctt
     1861 ccaccacgta acgggcccgc agttccatgg tggccagttt ggccaccagg agcggatcca
     1921 ggagaggagg tcgggggacg ggcgcctcgc tcataccgga agcgttgtca ggagggattt
     1981 ttcaggagac gctggatcac gtcgcgcacg cgaacgcctt cggcctcggc ccggaacgag
     2041 aggatgagcc ggtgcctcag gaccggttcg cacaaagcca aaacgtcgtc ccaggagacg
     2101 gcgaaacgtc cgttcaggat gcagcgcgcc ttggcgccca ggatgagcgc ctgagaggcg
     2161 cgcgggccgg ccccccagga aatccagtct ttcacgaatt cttccgcgcc gctctgcgga
     2221 cgcgtgcgcc gcaccaacgc cacggtcttc tccaccacgt tctccgccac cggcactttt
     2281 cggatcacct cctgcagccg caggatggct tccgcgtcca gcaccttctg caactccgcg
     2341 gcatagccgc cggtggtttg caggacgatt tccttctcct cttcggccga cggatagtcg
     2401 atgtttatct ggaacagaaa acggtccagt tgggcctcgg ggagcggata ggtcccctcc
     2461 tgctcgatgg ggttctgcgt ggccagcacg aagaacggca ggggcagcgg atgggtcttc
     2521 cccatcacgg tcacctggta ttcctgcatg gcctggagaa gggcggactg cgtcttgggc
     2581 ggggtgcggt tgatctcgtc ggccaggacg atgttggcga acaccgggcc gggcgcgaaa
     2641 cggaaagtgc gctcgcgggt gatgggatcc tcctgaagca cgtccgtgcc ggtgatgtcc
     2701 gacggcatga ggtcgggggt gaactggatg cggcggaact gcaggtccaa tatccgggcc
     2761 aagctggaaa tcaacaaggt cttggccagc ccgggcaccc ccaccaccac gcagtgaccg
     2821 cgggcaaaaa gcgccaccat caactgttcg atcaccgcgt cctggccgac gatgatcttg
     2881 gaaagttggc cgcggacctt cccgtacgcc tcgatgagcc ggtccacgag aacggaatct
     2941 ttttcctggg ccgccgattt atccatcgcg cctttcctcc ggtatttttc ctataaacgc
     3001 attgaatttt cgcttcgttt cagtataacg cttttccccc gtcctccgca actgtgattc
     3061 catcaagggt tccccaccct tgacgggggg ttccttttct ttgtacgcac aaagaaaagg
     3121 aacgaaaaga aatgcgcccc tgctcgccgt tcgctcaacg attttcggcg ccggcggaac
     3181 tcgcgggact caacgtccga cgacccgctc aaacatcctc cgacgaccgc gtcccgaaaa
     3241 tcgtttcggc tcataaacgg ctcaggggga ttatgaaacc ctttgcggga gcttcaggtt
     3301 tctttccctc ctgggaccct ggctgggccc tggggagaat caccccgcat cggttgtgcg
     3361 aaatgcggga attgatgagg agcgcaaacc tggcaaaccg cccaccactt aagaacacgt
     3421 cggagaccaa cctccccgaa gaatgccggt gatgtttttc agaccccctt aagggcgttt
     3481 tgaaggagga aattttcggt gctggaaggc cttggtgttc gagccctcat cagggccgtt
     3541 gatgagggca agttcaaggc cgcctaaaat tttcaactga tggcccgctg gggtgtcatt
     3601 tctttcggtc ccctttcttt ggacaagcaa agaaagggga cacaggtgca gggcggtatg
     3661 ccctgcgaat atttggccaa caacttccgc attgacaaca agcccttgcg gggatatctt
     3721 aaaaagaaag gctgatgaca tgacgggagg cgctatgcga ccgagttcga cgaaagtggt
     3781 gctgtgcggc ggtgtgcgga cgcccattgg gcacctggcg aaaagcctcg ccgggttcct
     3841 gcccgaagac ctgatggaga tcgtcatccg gggcattctg caaaaatcct ccctctatcc
     3901 ccacgcggtc gacggagtcc tggtgggctg ggtgggccag ggttcccacg cccccaacat
     3961 tgcccgcgtc gccctgctca aagcgggact gccggaaaaa gcccacgcgt tcaccatcca
     4021 agccaactgc gtctccagtc tggagagcgt ggcgtcggcc tatcggcaca tcatgatgtc
     4081 ggaaggggac ctctacctcg ccggcggcac ggaatccatg tccaccttcc cctacgccat
     4141 ccggggtccg cgcagccaca aatccctccg ttccctcgac gccgtgaaaa ccaactgggc
     4201 ggagctcctc aacacgccgg acgtctgcct caccgacacc accgaggaag ggctcaccga
     4261 ccccgtcaaa aagatctcca tggccgccac cgccgagatc tgtgcccaga tgtattccat
     4321 ttcccgggag gaacaggacg cgtacgccgc cgaaaccttc cggcgggcgc tggcggcgga
     4381 aaagaacggt ttttacgaca cccacaccgt gcccgtgaaa aaggacggcc ggaccgtcct
     4441 ggaccgcgac gaatacccct tcctgcggga agggctggtg gaaaaaccgc agatgttcgc
     4501 caaggcgccg gcgttgtttg aatcgggcgg gttcaccatg aaggactttt acgccaattt
     4561 cggcgagcac atcctgggca aaacctttga accgggaaaa tcccaaggga cggtcaccct
     4621 cttcaacgcc tgcgcccgct ccgacggcgc cgcggggctg gtggtggcgt cggaggaacg
     4681 ggccaaacag ttggggctgg agatattggc ggagatcaag tcgtgggggt tttggggcaa
     4741 caacccggcc cacatggggg tgtcgccggt gttcagttcc gccgtggcgc tggaacgcgc
     4801 gggactgaaa ttcgccgacc tggaccatgt ggaactgcac gaggcgttcg ccgccacctc
     4861 cttgtccatc ttcaaggtgg gcaaggagaa acacgggcag gattggcgga agttgtggga
     4921 ttcgggccgg ctcaacccga acggcgggtc catcccgctg ggccacccgc tggcggccac
     4981 gggggcgcgg ctgttactga acctgctcta cgcgatgaaa caggacccgg gcgcgcggtt
     5041 cgggttggtc gcggcctgcg cggccggagg gctcggcgga gcgatggtgg tggagaagta
     5101 tcggtaaacc ggcgggaaag gatatccccg agatcgtcgg ctagatcggt ttcgcctcgt
     5161 cgatcagcat caccgggatg ccgtctttga tggcataagc cagcttgcat ttctgacaca
     5221 gcagccggtt ctccgattcc cggtattcca aatccccctt acacttcggg caggccaaaa
     5281 tttccaacag ttcttttttc agcgccatcg tctcctcctc aaataatgaa cgtcatcccc
     5341 gcgaaggcgg gaacccagaa gcaatttttt ttgcccctcc gcctcccgcc tcgtcaaccg
     5401 agattcttag tcccccggcg acaattaaaa aattgggcga aagtgacgaa gtcgttcacc
     5461 ctcgcggatg aaattgtttg tgcatccgct tcaactggtc cttttccacg tgcgtgtaga
     5521 tctgggtggt ggagatgtcg gcgtgaccga gcatctcctg aacggaacgc aggtccgccc
     5581 ccccgcccaa gagatgcgtg gcgaacgaat gccgcagcat gtgcgggtgc aggggctttt
     5641 tcaagccgca gtcgcgcgcc cacttgcgga gttgatacca aaacgccacc cgcgacaggg
     5701 gcttgccgcg gttgcttaaa aaaacgtacc gcgcggcatt cggaaacttc ttccgcttca
     5761 cgtccagata cgcccccacc gactgaaccg cccggcggtg gatgggcacg atccgctcct
     5821 tgccgccttt cccgaaggcc ctcacgaacc ccacgtccaa atccagttgg gcctcttcca
     5881 ggttcaccag ttcgctcacc cgcaggccgg cggcgtacat caactccagc atggccttga
     5941 accggacgga ggattcccgt ccatcgggca cgcggttcaa gagccggccc acctcctcct
     6001 cgctcagata acgcgggaga cggtgcgtga gtttcggtga actcatccgg gccgtcgggt
     6061 cctcgctgat gagctcctca gcgagcagat aatgatagaa ctgtttgatg gattcggcga
     6121 gccggaaaag gctggcggat ttcagcgtct ggcggcgctg ccagaggtag tccgtgatct
     6181 cggcgtgcgg aatacggagc gggttttttt tgtgccggtg caggtgctcc aaaaaacggc
     6241 gcaggtcggc gccgtacgcg gccagggtgt tc
//
LOCUS       DAOTFD010000153         6247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8970_length_6247_cov_3.848676, whole genome shotgun sequence.
ACCESSION   DAOTFD010000153 DAOTFD010000000
VERSION     DAOTFD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8970_length_6247_cov_3.848676"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5079_08570"
     CDS             complement(<1..235)
                     /locus_tag="P5079_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRY30071.1"
                     /translation="MERTLMVGLQTGKSTADRRLPSLDELRRLIETAGGEVVDTVVQK
                     RAQRDPATLIGRGKAEEIAGLARQKTLDAVIFDD"
     gene            complement(260..1237)
                     /gene="miaA"
                     /locus_tag="P5079_08575"
     CDS             complement(260..1237)
                     /gene="miaA"
                     /locus_tag="P5079_08575"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY30072.1"
                     /translation="MSPIPILVGPTGSGKTAAGLELARRQGADVLSADSRQVYKHLSV
                     GTSKPEGAWRDDPDHPLKTYYAVDGVPHHLMDFLEPDQPFSAGRFARRAENLLAALAA
                     AERPALLVGGTGLYLRALVDGLAPLPDRNDDFRKTLLARAERNGRAAVHAELAKIDPD
                     AAAKIPPNNIARVVRALEVFHLTGQPLSRWQKEQTRPSTRTFAWFGLRWEKNELEKIL
                     EARCRRMVPGLLKETAALLEKGLPPDAPALQSLGYAAAIDVLNGKIPEEKFETDFFRE
                     TRLYVKRQLTWFRANPRIRWIDLAPPFKPERVAEKIQQLLRDASAAPGR"
     gene            complement(1276..2286)
                     /locus_tag="P5079_08580"
     CDS             complement(1276..2286)
                     /locus_tag="P5079_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY30073.1"
                     /translation="MNLRNHLRFAVIGCGRMGLRRMQSLVDNPRAELLWAVDVKDSAR
                     RTAAARHGCDTAARPDEAINDPAVDGVVVSVPNKWHRDIVLSALRRKKHVFCEKPLGR
                     DTAEARAMLAAAERSRVTLKVGSNLRYFPNVLKAKRLYDAGAVGRPLFLRSWIGHNGW
                     TQDGWYAVPRLSGGGPLLDNGAHLFDLLRWFLGEPVDAFGAAQPLPDGRSRVEDNAMA
                     VFHMKNGALASVHASWTQWAGYMRLELGGTRGALRIENSGPRCETVLVARSGKEKVFD
                     FSSQSPNSYQAEMDDYLARLLRKEQPRASGWDGLRAVQMARAVYRSALTGRRVRIEAP
                     PR"
     gene            complement(2283..3428)
                     /locus_tag="P5079_08585"
     CDS             complement(2283..3428)
                     /locus_tag="P5079_08585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY30074.1"
                     /translation="MKRPSSRSRDGFIPMAAPVMGPQELRYVSDVIKSGWISSEGRYV
                     SEFEKAFSRYCGVAHGVAVTSGTTALHLSLAVLGVGPGDEVLIPTLTHIAVPNAVKAV
                     GAVPVPVDSEPFTWNLDPALLERKISRRTRAVIVVHLYGHPTDMDPVRRFARKHGLFV
                     VEDAAESHGAQYKGRRTGSLGHLGCFSFYANKIIATGEGGMIVTDNARLARRARKLRD
                     QAYEKKRRFWHREHGFNYRMTNLQAAVGLAQLERIEQFIAARRRISALYNRLLRGVPG
                     LTPPPAAPWAASTHWMHALLVHKEFGLSRDRLMASLRRHGIDSRPFFYPVHLQPLYKK
                     ELGAHRCPVAESLSSIGMNLPTGSRMTEAEVRRIADTLLTLHRENSR"
     gene            complement(3425..5263)
                     /gene="mutL"
                     /locus_tag="P5079_08590"
     CDS             complement(3425..5263)
                     /gene="mutL"
                     /locus_tag="P5079_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRY30075.1"
                     /translation="MPQIQKLPEQLIRRIAAGEVIERPASVLKELLENALDAGARRIT
                     VEWAQAGRKKIRVTDDGAGIAPEQVALALERHATSKITDLEDLEKIRTFGFRGEALPS
                     IAAVSRFKLVSRTAEAAEGRSIECDGGKIVREGPCGAPAGTTVTVEDLFFCTPAREKF
                     LKSDTTERGLLLRTLEDVALAARDVAFDVITDGKELLSLRPAGPGADPAAALLERIKA
                     CWGADRFTALKPVHEVGRFMSVFGWISDVHAHQSTGRHQRFYVNNRPVLSRRLTHVLY
                     EAYRGGLMVGRHPAAVIFLSVDPTLVDVNVHPSKREVRLSHEEEAYGFLYHSLKKALS
                     TSAEMPSALAPAGRPAAPAAEKQTAPPHPPRHPESFKEKNLSLAESQAALFIQSPLKH
                     TPPEAAPPSVDNTLPETPSEDAAAPRPEDFRKTTPTPLMQMDGTYILARLGNDLYIFD
                     QHAAAERVLYEKFSENAKDQTPHRQVLLLPWLWEVSLETAAIVTDCLKDFERLGYRLE
                     PFGPQVFRVTAVPSVLGDSPKVRTLLEGLAEDLMSEAIPRRWDDILIRAACRGAVKAH
                     DPLQPSEMEKILKDLQLCRLPWSCPHGRPTFLRLSPDELAKKFRRI"
     gene            complement(5325..5759)
                     /locus_tag="P5079_08595"
     CDS             complement(5325..5759)
                     /locus_tag="P5079_08595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY30076.1"
                     /translation="MKTERRKHHRFPVIKDLAEPIDIFLMDEPARDLPAVLTNLSAGG
                     MSLMVFAHVSGDTRLKLVLNLPGLEGIELHGHVTWTSSKGDTTMLGVAFNHIPHDTVK
                     CINHMAEHYQDCELKLSFGLKDVCFRQCSYWPLCEKTVKLKH"
     gene            complement(5818..>6247)
                     /locus_tag="P5079_08600"
     CDS             complement(5818..>6247)
                     /locus_tag="P5079_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30077.1"
                     /translation="EVTDLADRLSGVKNHHAAVREWVRPDGRTELVLLHQILPGPADR
                     SYGVHVAQMAGLPRECVARARHILKTLEATHAVPGRRQKNDKPDGDPAQLPLFASHPV
                     LEELKGTDPNALTPLQALEILHRLVQDVASSQETPSKPQK"
BASE COUNT          978 a         1760 c         2348 g         1161 t
ORIGIN      
        1 cgtcgtcgaa gatgacggcg tcgagggttt tttgccgcgc gaggccggcg atctcctccg
       61 ccttgccccg gccgatgagc gtggccgggt cgcgctgggc ccgtttttgg acgacggtgt
      121 ccacgacctc gccgccggcc gtctcgatga gacgccggag ttcgtccaaa gacggcagac
      181 gccggtccgc ggtgcttttg ccggtctgga gaccgaccat cagggtgcgt tccatggcgg
      241 ctattttagc agtttctttt cagcggccgg gcgccgccga ggcgtcccgg aggagttgtt
      301 gtatcttttc ggcgacccgc tcgggtttga acggcggggc gaggtcgatc cagcggatgc
      361 gcgggttggc gcggaaccag gtgagctggc gtttcacgta gaggcgggtt tcgcggaaga
      421 aatcggtttc gaatttttcc tccgggattt ttccgttcag aacatcgatg gccgcggcgt
      481 aacccaggga ctggagggcc ggggcgtccg gcgggaggcc tttttccaag agggccgccg
      541 tttctttcag aaggccgggg accatgcgcc ggcagcgggc ctccaggatt ttttcgagtt
      601 catttttttc ccagcgcaat ccgaaccagg cgaaggtcct ggtcgagggg cgcgtctgtt
      661 ccttctgcca gcgcgagagc ggttgtcccg tgagatgaaa gacttccagg gcgcggacca
      721 cgcgcgcgat gttgttgggc ggaatcttgg cggcggcgtc cggatcgatt ttcgccaatt
      781 cggcgtgcac ggcggcgcgg ccgttccgtt cggcccgggc cagaagggtt ttccggaagt
      841 catcgtttcg gtcgggcaga ggggcgaggc cgtccaccag ggcgcgcaga tacagcccgg
      901 tcccccccac caggagggcc ggtcgttccg ccgccgcgag cgcggcgagg aggttttccg
      961 cccgacgggc gaaccggccg gcgctgaagg gttggtcggg ttcaaggaag tccatgagat
     1021 ggtgcggcac gccgtccacg gcgtaatagg tcttcagcgg gtggtccgga tcgtcccgcc
     1081 acgcgccctc gggtttggag gtgccgacgg aaaggtgttt gtagacctgg cgggaatcgg
     1141 cggagaggac gtcggctcct tggcggcggg ccagctccaa cccggcggcg gttttccccg
     1201 agcccgtggg gccgaccagg atggggatgg gagacatatc gattttcaat ggaagcgccg
     1261 tgccgcgggc ggaaatcagc gcggcggcgc ttcgatgcgg acccgccggc cggtgagggc
     1321 cgaccggtag acggcccggg ccatctgcac cgcgcgcaag ccgtcccagc cggaggcccg
     1381 gggctgttct tttcggagga gtcgggcgag gtagtcgtcc atctccgcct gataggagtt
     1441 gggcgactgg gaggaaaaat cgaagacttt ttctttgccc gagcgggcga cgaggaccgt
     1501 ttcacagcgg ggtccgctgt tctcgatacg gagcgcgccc cgggtgccgc cgagttccag
     1561 gcgcatgtaa cccgcccatt gcgtccagga ggcatgcacg gaggccaagg ccccgttctt
     1621 catatggaac acggccatgg cgttgtcctc cacccgggag cgaccgtcgg gaagaggctg
     1681 tgccgcgccg aaagcgtcga cgggttcacc caggaaccag cggaggaggt cgaagagatg
     1741 ggcgccgttg tcgaggagcg gacctccgcc cgagaggcgg gggacggcgt accagccgtc
     1801 ctgggtccag ccgttgtggc cgatccagga gcggaggaag agggggcggc cgacggcgcc
     1861 ggcgtcgtag agacgtttgg ctttgaggac gttggggaaa taccgcaggt tggaaccgac
     1921 tttcagggtg acgcgggaac gttcggcggc ggccagcatg gcccgggcct cggcggtgtc
     1981 ccggccgaga ggtttttcgc agaagacgtg ttttttccgt cggagagccg acaggacgat
     2041 gtcgcggtgc catttgttgg ggacggagac gacgacgccg tcgacggcgg ggtcgttgat
     2101 ggcttcgtcg ggccgcgccg ccgtgtcgca tccgtggcgc gcggcggcgg tccggcgggc
     2161 ggaatccttg acgtccacgg cccagagcag ttccgcccgc gggttgtcga cgagggactg
     2221 catccggcgc aggcccatcc ggccgcagcc gatcaccgcg aagcgaaggt ggttgcggag
     2281 gttcaccggc tgttttcccg gtggagggtg aggagggtgt cggcgatgcg gcggacctcg
     2341 gcctcggtca ttcggcttcc ggtggggagg ttcatcccga tggagctcaa cgattcggcg
     2401 acgggacagc ggtgcgcgcc gagttctttt ttgtagagcg gctggaggtg gacgggatag
     2461 aagaaaggcc gcgagtcgat gccgtgtcgg cgcagggagg ccatcagccg gtcccggctg
     2521 agcccgaatt ccttgtgcac gagcagggca tgcatccaat gggtgctggc ggcccagggg
     2581 gcggcgggcg gcggggtgag gccgggcacg ccgcgaagga gccggttgta gagggcggat
     2641 atccgtcgcc gtgcggcgat gaattgttcg atgcgttcca actgagccag ccccaccgcg
     2701 gcctgcaggt tggtcatgcg gtaattgaag ccgtgttccc ggtgccagaa tcggcgtttc
     2761 ttttcgtagg cttggtcgcg cagtttgcgg gcgcgccggg cgagccgggc gttgtcggtg
     2821 acgatcatcc ccccctcgcc ggtggcgatg attttgttgg cgtaaaaact gaaacagccg
     2881 agatggccga gacttccggt tcggcggcct ttgtattggg cgccgtggga ctcggcggcg
     2941 tcctccacca cgaagaggcc gtgcttgcgg gcgaaccgcc ggaccgggtc catgtccgtc
     3001 ggatggccgt aaagatgcac gacgatgacg gcccgggtcc gccgggagat ctttctttca
     3061 aggagggccg gatccaggtt ccacgtgaag ggttcgctgt cgacgggcac ggggacggcg
     3121 ccgaccgcct tgacggcgtt gggaacggcg atgtgagtga gggtgggaat gaggacctcg
     3181 tcgccggggc cgacgcccag aacggcgagc gacagatgga gcgccgtggt gccgctggtg
     3241 accgcgacgc cgtgcgccac gccgcagtag cggctgaacg ctttttcgaa ttcggaaaca
     3301 taacgtcctt cggacgagat ccagccgctc ttgatgacgt cggagacgta gcggagttct
     3361 tgggggccca tgaccggggc ggccatgggg atgaatccgt cgcgggaacg ggaggacgga
     3421 cgtttcatat ccgccggaac tttttcgcca gttcgtccgg ggaaaggcgg aggaacgtgg
     3481 gccggccgtg ggggcacgac cacggcaggc ggcagagttg gaggtccttc aggatttttt
     3541 ccatctccga aggttgcaaa gggtcgtgcg ctttgacggc gccccggcag gcggcgcgga
     3601 tgaggatgtc gtcccatcgg cgcgggatgg cctcggacat caggtcctcc gccagtcctt
     3661 ccaagagagt gcgcactttc ggggaatccc ccaggacgct ggggaccgcg gtgacccgga
     3721 agacctgcgg cccgaacggt tccagccgat atcccagccg ctcgaaatcc ttaaggcagt
     3781 cggtcacgat ggcggcggtt tcgagggaga cctcccagag ccaggggagc agaaggacct
     3841 gccggtgcgg ggtttggtct ttggcgtttt ctgaaaattt ctcatagagg acgcgctcgg
     3901 cggcggcgtg ctggtcgaaa atgtaaaggt cgttgcccag ccgggccaga atgtaggtgc
     3961 cgtccatttg catgagcggc gtgggcgtcg tcttgcggaa gtcctccggc cggggcgcgg
     4021 ccgcgtcctc ggagggcgtt tccgggaggg tattgtcgac ggacggaggg gccgcctccg
     4081 gcggcgtgtg tttcagcggg gactggatga agagggcggc ctgggactcg gccagggaca
     4141 ggtttttttc cttgaacgat tcgggatgac gcggaggatg gggaggagcg gtttgttttt
     4201 cggccgccgg cgccgcgggc cggccggccg gggccaacgc gctcggcatt tcggccgacg
     4261 tggaaagggc ttttttcagg gaatggtaga ggaacccgta ggcctcctct tcgtgcgaaa
     4321 gccggacctc gcgtttggac ggatgcacgt tgacgtccac caaggtcggg tccacgctga
     4381 ggaagatcac cgccgcgggg tggcggccca ccatcaaccc gccgcggtag gcctcgtaga
     4441 ggacgtgggt gagccggcgg ctgaggacgg ggcggttgtt cacgtaaaag cgttgatggc
     4501 ggccggtgct ttggtgcgcg tggacgtcgg agatccagcc gaagacgctc atgaagcggc
     4561 cgacctcatg gacgggtttc agggcggtga atcgatcggc gccccaacag gccttgatcc
     4621 gttcgaggag ggccgcggcg gggtcggcgc ccggcccggc cgggcgcaac gagagcagtt
     4681 ccttgccgtc ggtgatgacg tcgaaggcga cgtcccgggc ggcgagggcg acgtcttcca
     4741 gggtgcgcag gagaaggccg cgttcggtgg tgtcggactt gaggaacttt tcccgcgccg
     4801 gggtgcaaaa gaacaggtcc tccaccgtga cggtggtgcc cgcgggggcg ccgcaggggc
     4861 cttcgcggac gattttcccc ccgtcgcatt cgatggagcg gccctcggcg gcctcggcgg
     4921 tgcgggagac cagtttgaaa cgggagaccg ccgcgatgga aggaagcgct tccccgcgga
     4981 aaccgaaggt gcggatcttc tccaggtctt ccagatcggt gatcttggag gtggcgtgcc
     5041 gttcgagcgc cagcgccacc tgttccggcg cgatgccggc gccgtcgtcg gtgacgcgga
     5101 tttttttccg gccggcctgc gcccattcca ccgtgatgcg ccgggcgccg gcgtccaggg
     5161 cgttctccag aagttccttc aggacggagg cggggcgttc gatcacctcg ccggcggcga
     5221 tccgccgtat cagttgttcg ggaagttttt gtatttgagg catgtcgggc aataaaagga
     5281 gacgcggcgt tccggcggaa ggtgtccgaa aggagagagg aacgtcagtg tttcagtttg
     5341 accgtctttt cacacaacgg ccagtaggaa cattggcgga agcagacgtc tttcaatccg
     5401 aaggaaagtt tgagttcgca gtcctgatag tgttccgcca tgtggttgat gcatttgacc
     5461 gtgtcgtggg ggatgtggtt gaaggccacg ccgagcatgg tggtgtcgcc tttcgaactg
     5521 gtccaggtga cgtggccgtg gagttcgatc ccctccagcc ccggcaggtt gaggacgagt
     5581 ttgaggcggg tgtcgccgga gacgtgggcg aagaccatca gcgacatgcc gccggcggag
     5641 aggttggtga gcacggcggg caggtcccgc gccggttcgt ccatgagaaa gatatcgatc
     5701 ggttcggcca ggtcctttat gaccggaaaa cgatggtgtt tgcgtctttc cgttttcacg
     5761 tcacccctcg aaattgtcgc ttcggaaatt gtccatcttt catttgaaaa aggcaaatca
     5821 tttctgcggt ttggacggcg tttcttgtga agaggcgacg tcctggacca gccggtggag
     5881 gatctccagg gcttgcaggg gcgtgagggc gttgggatcg gtgcccttta attcctcgag
     5941 gacgggatgg gaggcgaaga ggggcagttg ggcggggtcc ccgtcgggct tgtcgttttt
     6001 ctgccgccga ccggggacgg cgtgggtggc ttcgagggtc ttgaggatgt gccgcgcgcg
     6061 cgccacgcat tcccgcggga ggccggccat ctgtgccacg tgcacgccgt aggaacggtc
     6121 ggccgggccg ggcaatatct ggtggaggag caccagttcc gtgcggccgt cggggcggac
     6181 ccattcccgg acggcggcgt ggtggttctt gacgcccgac aggcggtcgg ccaggtcggt
     6241 cacctcg
//
LOCUS       DAOTFD010000154         6246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_8972_length_6246_cov_4.407850, whole genome shotgun sequence.
ACCESSION   DAOTFD010000154 DAOTFD010000000
VERSION     DAOTFD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_6246_cov_4.407850"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /gene="mutS"
                     /locus_tag="P5079_08605"
     CDS             complement(<1..2353)
                     /gene="mutS"
                     /locus_tag="P5079_08605"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRY30078.1"
                     /translation="MSDPMPNDPSAAKTPASPEPADTVNLPDSKQTPLMRQYQALKTK
                     NPEALLFFRLGDFYELFYDDARKAAPLLEVVLTQRQGIPMCGVPHHALAGYLAKLLRL
                     GLRVAIAEQMEDPATTKGMVQRDVVRVVSPGTVLEDELLQAKQNNFLVAVWPDRAAKP
                     AGWGIAALDLSTGEFRLTAVPRDLDGHRLADELSRLDPREILWPASAGGALPSVGRPV
                     TPLDDRAFQPDSAERLLSRTLNVTSVAGFGLDRRHPSLPAAGAVLSYVEQNQPAALSV
                     VQPPRLYQLDDYLGLDDIALRHLDLYPSERTPPGNGGPRTLWDVLDHTATPMGGRKLK
                     WWLFHPLRDPEAIRRRQDGVEFFLEHGVQRRDQRDLLKETSDVERILARLTAGGANGR
                     DLSALRRTLQRMPDLRRLYADKTSLAGEPHPLESFLRSLAVPAELLALLEKSLADDPP
                     FRLTDGGVIRDGYSPALDDLRRLAKHGRSWISEMENQERQRTGIGSLKVGYTSVFGYY
                     LEVSRANLSKVPPEWIRKQTLVNAERFITPELKVQEDKILGAEEKSKALEAELFAEVR
                     TGVLQHRETLRALAEALAELDTLASLAEAAERGHYRRPRVIDGNIFRLEKARHPVVEK
                     SLASLGGTPFVPNDTLLDGQENQILLVTGPNMGGKSTYLRQVALLAIMAQMGSFVPAE
                     TAEIGVLDRIFTRIGAGDNLAAGASTFLLEMQEVSNILHNATPRSLVILDEVGRGTST
                     YDGVAVAWAVVEYLHRGEKNAGPKVLFATHYFELTDLADRLSGV"
     gene            complement(2346..2969)
                     /locus_tag="P5079_08610"
     CDS             complement(2346..2969)
                     /locus_tag="P5079_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY30079.1"
                     /translation="MTHPPLPPPGGTPDLPQKSPTTRRRLFWVSAAVLLVVGLFLLLQ
                     NLDFYWNLTRPALHKDIINKYAAVYKFDPLFVMALVKVESRFAETAHSHRGAVGLMQL
                     MPTTALDMAKRLGEKVTLAALNRPETNIRLGFHYLSLLRTEFKDDTVALLAAYNAGPA
                     NVRTWMKAGPLMLTTIPYLETRLFIEKVLNTYAWLKRFQKIKIFFRV"
     gene            complement(2966..3196)
                     /locus_tag="P5079_08615"
     CDS             complement(2966..3196)
                     /locus_tag="P5079_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HRY30080.1"
                     /translation="MIKFAAVLVLLGALLAVAIQNPHPVTVNFLAWKSMSTTLLVVVL
                     VGLLVGLVGGFFIGFRTGKRDRPIEEEEDDPE"
     gene            complement(3189..4568)
                     /gene="miaB"
                     /locus_tag="P5079_08620"
     CDS             complement(3189..4568)
                     /gene="miaB"
                     /locus_tag="P5079_08620"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRY30081.1"
                     /translation="MNPVIEMSVNPTPRARTAPPVPAGRPRFYIETFGCQMNAADSEE
                     MSRTLRRRGFIAADSPADADLFIINTCTVRQHAENRALSFLGRLRRWKDERPGRLLVV
                     AGCVAERLKKELPSRFSHLDGVVGAKSMDRFDTLVEKLLLPAEGPARLPDPGRAQVTA
                     HVTVMRGCNFSCSYCIVPSVRGRESHRPLKNILEEIREKTAEGTPEVLLLGQTVNSYR
                     GDGPAGTADFADLLRAVQKIDAVRRIRYMSPHPFYVNDRFLAALAECDKVCPHIHLPL
                     QSGSDRLLKRMRRNHTRADFLDRVRRLRRARPEILITTDLIAGFPGETDDDFNQTVSL
                     VRESAVDGAFCFKYSPRPGTPAAEFPDDVPETVKEERHAELLKIVTAHSERGIASLVN
                     TRQDILIEDVKSPGRDRTGRIVPVYEGRTRTCWKTFFTSSRNHRRGDIVPVQIAEAVG
                     RNLWGDTRD"
     gene            4718..5602
                     /locus_tag="P5079_08625"
     CDS             4718..5602
                     /locus_tag="P5079_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY30082.1"
                     /translation="MNAEGLQKIFFCLGLALFLSGCRAERLYYYPNAELYADPHNVGL
                     EYEIVNYPSLNGKTLYGLYFQTAGVPRGTIVHFHGNYGNVSNHFPLSVFLLKYGFDVL
                     IFDYQGYGASAGHPSPKSTVEDGVASVRYALSRRRTPEGGLGVFGQSLGAAVATVVAA
                     EEPAVDAAVLEAGFTTYRAIGKDVLKRSLLTWPFAYIAPPLFVGRRCDPLDHIGRISP
                     RPVLLIHGTKDRTIPLWMSRRLFERAKEPKTLWVVEGGEHLGYKRVAGTPYERAVSDF
                     FQRAFEEKQRRLRDNSSR"
     gene            complement(5580..>6246)
                     /locus_tag="P5079_08630"
     CDS             complement(5580..>6246)
                     /locus_tag="P5079_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30083.1"
                     /translation="AGHPSGRISLGYGFNDDTEFEELSLRPALHDLPADPAGYIPNSQ
                     LEMFHLLLRYNNDRGHVYVERLGLLDIVSLAPLDRWIKRPSWKFFTGWGNAKDTGRPP
                     DKGLHFDLNAGTGPTLETSMWKKETFYLLAESDLGVGGLFRRNYRWGVGGTAGCLVDV
                     TDAWRVVLEETYLRYPLGEVRSRTRTDLTQSFTLNRRTELRLILRRDGPWKETLLTAN
                     YYL"
BASE COUNT         1028 a         1777 c         2265 g         1176 t
ORIGIN      
        1 tgacgcccga caggcggtcg gccaggtcgg tgagttcgaa gtaatgcgtg gcgaagagca
       61 ctttgggccc ggcgtttttt tctccgcgat ggaggtattc caccacggcc caggccacgg
      121 ccacgccgtc gtaggtggag gtgccgcgcc cgacctcgtc caggatgacg aggctgcgcg
      181 gggtggcgtt gtgaaggatg ttggaaacct cctgcatttc caggaggaag gtggaggcgc
      241 cggcggccag gttgtctccg gcgccgatgc gggtgaagat gcggtccagc acgccgatct
      301 ccgccgtttc ggccgggacg aaggatccca tctgcgccat gatggccagg agggccacct
      361 ggcgcaggta ggtggatttg ccccccatgt tcggcccggt gacgaggagg atctggttct
      421 cctgtccgtc caggagcgtg tcgttgggca cgaacggcgt tccgccgagg ctcgccaggg
      481 atttttcgac cacggggtgg cgggcttttt cgaggcggaa gatgttcccg tcgatgacgc
      541 gggggcggcg gtaatggccc cgttcggccg cctcggccag ggaggcgagg gtgtccaact
      601 ccgccagggc ttcggccagg gcgcgcaggg tctcgcggtg ttggagcacg ccggtccgaa
      661 cctcggcgaa gagttcggcc tccagcgcct tggatttttc ctccgccccg aggatcttgt
      721 cctcctggac tttcagttcc ggggtgatga aacgttccgc gttcaccagg gtctgtttgc
      781 ggatccattc cggcggcact ttggagaggt tggcgcggga gacttccagg tagtaaccga
      841 agaccgaggt gtagccgact ttcaaggagc cgatgccggt ccgctggcgt tcctggttct
      901 ccatctccga tatccaggac cgaccgtgtt tggccagccg gcggagatcg tcgagtgcgg
      961 gcgagtaccc gtcgcggatg acgccgccgt cggtgagccg gaagggcggg tcgtcggcca
     1021 gggatttttc caacagggcg agcagttcgg ccggaacggc gagggagcgc aggaaggact
     1081 cgagcggatg cggttcgccg gcgagggagg tcttgtcggc gtagagccgc cggaggtccg
     1141 gcattctttg cagcgttcgt cggagggcgc tgaggtcccg tccgttggcg ccgccggccg
     1201 tgaggcgggc caggatccgt tcgacgtcgg aggtctcctt gaggaggtcc ctctggtcgc
     1261 ggcgctggac gccgtgttcg aggaagaact ccacgccgtc ctgtcggcga cggatggcct
     1321 ccggatcgcg cagcgggtga aagagccacc acttcagttt tcggccgccc atgggggtgg
     1381 cggtgtggtc gaggacgtcc cagagggtgc gcggcccgcc gttcccgggc ggggtgcgtt
     1441 ccgagggata caggtccagg tggcggaggg cgatgtcgtc gagccccagg tagtcgtcga
     1501 gctgatagag gcgcggcggc tggaccacgc tgagagcggc gggctggttc tgttccacgt
     1561 aggagagcac ggcgccggcg gccggcaggg acggatggcg gcggtcgagt ccgaacccgg
     1621 ccaccgaggt cacgttcaac gtgcgcgaaa ggaggcgttc ggccgagtcg ggctggaagg
     1681 cccggtcgtc caacggcgtc acggggcggc cgacggaggg gagcgcgccg ccggccgagg
     1741 cgggccagag gatctcgcgg ggatccaggc gggaaagttc gtcggccagc cggtggccgt
     1801 cgaggtcccg cgggacggcc gtgagacgga attctccggt ggaaagatcc agggcggcga
     1861 tgccccaccc ggcgggtttg gcggcccggt cgggccacac cgccacaagg aaattgttct
     1921 gtttcgcctg gagcagttcg tcttccagga cggtgccggg gctgaccacg cgcaccacgt
     1981 cccgttggac catccccttg gtggtggccg ggtcctccat ctgttcggcg atggcgaccc
     2041 gcagtccgag ccgcaaaagt ttcgccagat accccgccag ggcgtgatgc ggcacgccgc
     2101 acatggggat gccttgccgt tgggtgagga ccacctccag aagcggggcg gctttccggg
     2161 cgtcgtcgta aaagagttcg tagaaatcgc ccagccggaa gaaaaggagg gcctcggggt
     2221 ttttcgtctt gagcgcctga tattggcgca tcaggggggt ttgttttgag tccggcaggt
     2281 tcacggtgtc cgccggttcg ggggaagcgg gcgttttggc ggcggagggg tcgttcggca
     2341 tgggatcaga cacggaagaa gatttttatc ttttggaacc gtttcagcca ggcgtaggtg
     2401 ttgagcactt tctcgatgaa gaggcgggtt tccaaatagg ggatggtcgt cagcatcagc
     2461 gggcccgcct tcatccacgt ccggacgttg gcgggtccgg cgttgtaggc ggccaaaagg
     2521 gcgacggtgt cgtccttgaa ctccgtgcgc agaagggaga ggtaatggaa ccccaggcgg
     2581 atgttcgttt cgggacggtt caaagcggcg agggtgactt tctctcccaa ccgcttggcc
     2641 atgtccaagg cggtggtggg catcagctgc atcaacccca cggcgccccg gtgcgagtgg
     2701 gcggtctcgg cgaagcgcga ctccactttg acgagcgcca tcacgaagag cggatcgaat
     2761 ttgtagaccg cggcgtattt gttgatgatg tccttgtgca gcgccggccg cgtcaggttc
     2821 cagtaaaaat ccaggttctg aagcaaaagg aaaaggccga cgacaaggag aacggcggcc
     2881 gaaacccaga aaaggcgtcg gcgggtcgtc ggggattttt gagggaggtc cggggtccct
     2941 ccggggggcg gaagtggggg gtgggtcatt cggggtcgtc ctcttcttcc tcgatcggcc
     3001 ggtcccgttt tcccgtccga aatccgatga agaagccgcc gacgagcccc accagcaggc
     3061 cgacgagaac gacgacgagg agggtggtgg acatggattt ccatgccagg aaattcaccg
     3121 tcacggggtg agggttctgg atggccacgg ccagcagggc tcccaaaagg acgagaacgg
     3181 cggcgaattt aatcacgggt gtctccccag agatttcggc cgaccgcctc ggcgatttgc
     3241 acggggacga tgtccccccg ccggtggttt cggctcgacg tgaaaaaggt cttccagcag
     3301 gtgcgggtgc gtccctcgta gacggggacg atgcgtccgg tccggtcccg ccccggcgat
     3361 ttgacgtcct cgatcaatat gtcctggcgg gtattcacca gggaggcgat cccgcgttcc
     3421 gagtgggccg tgacgatctt caggagttcg gcgtggcgtt cctccttgac ggtttccgga
     3481 acgtcgtccg ggaactcggc ggcgggggtg ccgggccgcg gcgaatattt gaaacaaaaa
     3541 gcgccgtcga cggcgctttc ccggacgagg gagacggttt gattgaaatc gtcgtcggtt
     3601 tcgccgggga atccggcgat gaggtcggtg gtgatgagga tttccggccg cgcccgccgg
     3661 aggcgccgga cgcggtccag gaaatcggcc cgcgtgtggt ttcggcgcat gcgtttcagg
     3721 aggcggtccg agccggactg caggggcagg tggatgtgcg gacagacctt gtcgcattcg
     3781 gccagggccg cgaggaagcg gtcgttgacg tagaacgggt gcgggctcat gtagcggatg
     3841 cggcggaccg cgtctatttt ttggacggcg cgcaggaggt cggcgaaatc cgccgtgccc
     3901 gccgggccgt ccccgcggta ggagttgacg gtctggccga ggagcagcac ctccggcgtt
     3961 ccctccgccg ttttttcgcg gatttcctcc aagatgtttt tcagggggcg gtgggattcc
     4021 cggccccgca ccgacggcac gatgcaatag ctgcaggaaa aattgcagcc gcgcatgacg
     4081 gtgacgtgcg cggtgacctg cgcgcgcccc ggatccggga ggcgcgcggg gccctcggcg
     4141 gggaggagca gtttctccac gagcgtgtcg aaccggtcca tggatttggc gcccaccacc
     4201 ccatcgaggt gcgaaaagcg ggacggcagt tcttttttca ggcgttcggc gacgcatccg
     4261 gccaccacga ggagccggcc gggccgttcg tctttccaac gccggagccg gccgaggaag
     4321 gacaacgccc ggttctccgc gtgttgccgg acggtgcagg tgttgatgat gaaaaggtcg
     4381 gcgtcggcgg ggctgtccgc ggcgatgaat ccgcgccggc ggagggttcg cgacatctct
     4441 tcggaatcgg cggcgttcat ctggcagccg aaggtttcga tataaaagcg ggggcgtccc
     4501 gccgggacgg gaggcgcggt gcgggcgcgg ggggtcgggt tgacggacat ttcgatgacc
     4561 gggttcatat cctgttggag aagtctttag agttttttcg aagagggtct attctaccag
     4621 attctgggcc gggccgggga aggcgggtcg gcggattttc aggtcatcga gcaaaacgat
     4681 tttcccgccg gtcgaaataa agtataaaaa cggggtcatg aacgcggagg ggcttcaaaa
     4741 gatatttttc tgtctggggc tggcgctttt cctttcgggg tgtcgcgccg agcggctgta
     4801 ttattatccc aacgccgaac tctatgccga cccgcacaac gtggggctgg agtatgagat
     4861 cgtcaactac cccagtttga acgggaagac cctctacggc ctgtattttc aaacggccgg
     4921 cgtccctcgg gggacgatcg tccatttcca cggcaattac gggaacgttt cgaaccactt
     4981 tccgctttcg gtgtttttat tgaaatacgg gttcgatgtg ttgatcttcg attaccaggg
     5041 atacggcgcc tcggcggggc atccctcgcc gaaatcgacg gtggaggacg gggtggcgag
     5101 cgtgcgttac gcgttgtctc gaaggagaac gccggagggc ggtctggggg tgttcgggca
     5161 atctctgggg gcggcggtgg ccacggtggt ggcggcggag gaaccggcgg tggacgccgc
     5221 ggtgttggag gcggggttca ccacctaccg ggccatcggc aaggacgttc tgaaacggag
     5281 ccttctcacc tggccgttcg cttacatcgc cccgcctctc ttcgtcggcc gccgctgcga
     5341 cccgctcgat cacatcggcc ggatctcccc ccggcccgtc ctcctcatcc acggcaccaa
     5401 ggaccggacc atcccgctgt ggatgtcccg ccgtcttttt gagagggcga aggagccgaa
     5461 gacgttatgg gtcgtggaag gcggcgaaca tctgggatac aagcgggtgg cggggactcc
     5521 gtatgagcgc gccgtgtcgg atttctttca gcgggctttc gaggaaaaac agcgccggct
     5581 cagagataat agttcgcggt gagcaacgtc tccttccatg ggccgtcccg ccggaggatg
     5641 aggcgcagtt cggtcctccg gttcagggtg aaggactgcg tcaggtcggt ccgcgtgcgc
     5701 gaccgcacct cccccagagg gtaacgcagg taggtttctt ccagaacgac gcgccaggcg
     5761 tccgtcacat ccacaaggca tcccgcggtc cctcccaccc cccaccggta attccgtcgg
     5821 aacaatcccc ccacgcccag gtcgctttcg gccaggaggt agaaagtttc tttcttccac
     5881 atcgaggttt ccagcgtggg acccgtgccg gcgttgaggt cgaaatggag gcctttgtcg
     5941 ggcgggcgtc cggtgtcctt ggcgttgccc catccggtga aaaatttcca ggaaggccgt
     6001 ttgatccagc ggtccagcgg ggccagcgag acgatgtcca acaggcccag ccgttccacg
     6061 tagacgtggc cgcggtcgtt gttgtagcgg aggaggaggt ggaacatctc cagctggctg
     6121 ttggggatgt agccggccgg atcggcggga aggtcgtgga gggcgggtcg cagggagagc
     6181 tcctcgaatt ccgtgtcgtc gttgaaaccg taacccaggc tgatccgtcc ggacggatgg
     6241 ccggcc
//
LOCUS       DAOTFD010000155         6147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9156_length_6147_cov_4.893959, whole genome shotgun sequence.
ACCESSION   DAOTFD010000155 DAOTFD010000000
VERSION     DAOTFD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9156_length_6147_cov_4.893959"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /gene="ftsH"
                     /locus_tag="P5079_08635"
     CDS             <1..1512
                     /gene="ftsH"
                     /locus_tag="P5079_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY30084.1"
                     /translation="GKQAMAFGRSRAKLQTGKKQKVTFADVAGCDEAKEELQEIIEFL
                     KDPAKFQKLGGKIPKGVLLFGAPGTGKTLLAKAVAGEANVPFFSSSGSDFVEMFVGVG
                     AARVRDLFEQGRKSTPCLLFVDEIDAVGRHRFAGIGGGHDEREQTLNQLLVEMDGFDT
                     KEGVILIAATNRPDVLDPALLRPGRFDRQIAVPIPDLKGREQILRVHAKEIKLGPGVD
                     LSIIARRTPGFVGADIANLVNESALLASRRNKQWVEMKELEEAIDRVIAGPERKSRMM
                     SEKEKNIIAYHESGHTLIAKLLPTSDPVHKVSIIPRGPALGYTLQLPTEDRYLTTKSE
                     ILNRLCVLMGGRCAEQMMFNDVTTGAQDDLTKVTEIAHKMVCEYGMSEKMGPITYRKN
                     QQEMFLGRDLASAPPYSDHTAEAIDEEIKEIIASCQKKVSDLLKKNRPKLEALAKALI
                     EKEVLTGEEINRLVAGEPLDKPVSGLPGADPALPGEEAGPALGGAPEPAPRPT"
     gene            1569..2429
                     /gene="folP"
                     /locus_tag="P5079_08640"
     CDS             1569..2429
                     /gene="folP"
                     /locus_tag="P5079_08640"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRY30085.1"
                     /translation="MILRTRSKDLDLSHHTHLMGVVNVTPDSFSDGGKYFDAPAAVAH
                     AEELIRDGADLLDVGGESSRPGADPVPLDEELRRVIPVIQELARRHPSVPLSIDTTKA
                     EVARRAMRAGASLLNDISALRFDPGMAAVARETDVPVVLMHMQGNPRTMQTAPSYIDV
                     VREIGSFFRERLAFAEKTGIAADRLLLDPGIGFGKTLDHNLQILKNLRALAEIGRPLV
                     LGLSRKTFIARLLADGEKILSPQERSEGSLAGHLWAAAQGAHILRVHDVRGTRRALRV
                     WQAIARGLAG"
     gene            2487..3029
                     /locus_tag="P5079_08645"
     CDS             2487..3029
                     /locus_tag="P5079_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRY30086.1"
                     /translation="MFRRNYIVKPMLQLKYLLYGLGLVLVSGLCVYFIMDLAIVHSER
                     LSQLSQGEILSVQAAARVALLWALILLLLIVTVGTILIFHRLIGPVYAVEKVLRMVAD
                     GDLTVYMPLRKGDELREMAEAVEQMVGGIRRRLLHDKENALACQTKLRQIAEGNPSLK
                     AELNDIAAKLDRVGQEFRLG"
     gene            3158..4804
                     /gene="nadB"
                     /locus_tag="P5079_08650"
     CDS             3158..4804
                     /gene="nadB"
                     /locus_tag="P5079_08650"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRY30087.1"
                     /translation="MPPSPVVSDFLIIGSGIAGLSLALRAAQWGDVVLVTKLEIQESA
                     TAKAQGGLAVVVSPEDSFEQHIRDTLDAGAGLCREDVVRHVVVNAPARVQDLMQWGIR
                     FSETETHGPWEEPNFALGLEGGHSRRRILHVGDYTGRAIEEALVERVRENPRIRVFEY
                     HVAIDLITRRQLEKKDIPAQDNVCGGAYVLNVETGEIIPFLARGTALCTGGAGKVYLY
                     TSNPNIATGDGMAIAYRAGATLANMEFVQFHPTCLYHPKAKNFLISEALRGEGAVLKN
                     RAGERFVQKALPQAELAPRDLVSRAIDAELKRTGDDYVFLDISHRGADFIRRRFPSIY
                     ERCAAFDIDITAQPIPVVPAAHYFCGGVLTDIRGNTTLPRLAAVGETACTGLHGANRL
                     ASNSLPEALVFAHAVAETWQTLRDSPPPFPAGDVPPWVFALHPTSERVVIPHAWEEIR
                     RFMWNYVGIARTSLRLDRALQRIVLLENEIREHYDHAPVSPDLLELRNIAVVADTVIR
                     CALGRKESRGLHYNLDFPAPDDAGKKDSLISLSSDPAGHA"
     gene            4810..>6147
                     /locus_tag="P5079_08655"
     CDS             4810..>6147
                     /locus_tag="P5079_08655"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY30088.1"
                     /translation="MDADVIRIRGAREHNLKNITLDIPRNKLVVITGLSGSGKSSLAF
                     NTIYAEGQRRYVESLSAYARQFLELMEKPDVDLIEGLSPAIAIEQRTPSHNPRSTVGT
                     VTEIYDYLRLLFARIGAPHCPRCGREIRPQSASQIVTEVLKNHAGQKIQVLAPLISGR
                     IGTYEALFARLRKLGFARMRVDGQVADLHQKIKLNRYKKHTIEVVVDRLQVSPEGRER
                     VVDSVETALREAHGLVLLLPEGDKTGKGRLFSERHACAHCGLSLPEIEPRLFSFNSPY
                     GACADCDGLGVKLEVAEELIVPDTSLSVRDGALQAWANPVTTRTNRWKKSWAGYYVEI
                     LEGVCRRHHIPMDKPWRHMSRQHQDILLHGGVDHRSPWSSSDKEFEGVVRNLERRYRE
                     SESDFVKEAIRERFMRSRLCPTCRGARLKPEALAVSVAEKSIAEVARLWGVDSV"
BASE COUNT         1178 a         2212 c         1761 g          996 t
ORIGIN      
        1 gggaaacagg ccatggcgtt cggccggtcc cgcgcgaaac tgcagaccgg gaagaaacag
       61 aaggtgacct ttgcggacgt ggccggctgt gacgaggcga aagaggaact gcaggagatc
      121 attgagttcc tgaaagaccc cgccaaattc cagaaactcg gcggaaagat acccaaaggg
      181 gtgctcctct tcggcgcgcc ggggaccggg aagacgttgc tggccaaggc cgtggcggga
      241 gaggccaacg tgccgttctt ctcgtcctcc ggatcggatt tcgtggagat gttcgtgggc
      301 gtcggcgccg cccgcgtgag ggacctgttt gaacagggac ggaagagcac tccctgcctc
      361 ctcttcgtcg acgaaatcga cgccgtcgga cggcaccgct tcgcgggcat cggcggcggc
      421 cacgacgaac gggaacagac tttgaaccag ttgctcgtcg agatggacgg tttcgacacc
      481 aaggagggcg tcatcctcat cgccgccacc aaccgccccg acgtcctcga cccggcgctc
      541 ctgcggcccg gccgcttcga ccggcagatc gcggtgccca tccccgacct gaaggggcgg
      601 gaacagatcc tgcgcgtgca cgccaaggag atcaaactcg gccccggcgt ggacctcagc
      661 atcatcgccc gccgcacccc cggcttcgtc ggcgccgaca tcgccaacct ggtcaacgaa
      721 tccgccctgc tggcctcccg ccgcaacaag cagtgggtgg agatgaagga gttggaagaa
      781 gccatcgacc gcgtcatcgc cggcccggaa cggaaaagcc gcatgatgtc ggaaaaggaa
      841 aaaaacatca tcgcctatca cgagtccggc cacacgctga tcgccaaact cctgccgacc
      901 tccgatcccg tgcacaaagt ctccatcatc ccgcgcggac cggccctggg atacaccctc
      961 caactcccca cggaggaccg ctacctgacc accaaatccg agatcctcaa ccggctctgc
     1021 gtgctgatgg gcggccgctg cgctgaacag atgatgttca acgacgtgac caccggtgcc
     1081 caggacgacc tcaccaaggt cacggagatc gcccacaaga tggtctgcga gtacggcatg
     1141 tccgaaaaga tggggcccat cacctaccga aaaaaccaac aggagatgtt cctcggccgc
     1201 gacctggcct ccgccccgcc gtattcggac cacaccgccg aagccatcga cgaggaaatc
     1261 aaggagatca tcgcctcttg ccaaaaaaag gtgagcgacc tgttgaaaaa gaaccgcccc
     1321 aaactggaag cgctggccaa ggccctgatc gaaaaagagg tcctcaccgg cgaagagatc
     1381 aaccgcctcg tcgcgggcga gccgctggac aaacccgtgt ccggcctgcc cggcgcggac
     1441 cccgccctcc ccggcgagga agccggcccg gccctcggcg gcgccccgga acccgccccc
     1501 cggcccacgt aacggcaaat accgaaatac gagattttcg tgtttcggat cttagctttc
     1561 gtatttccat gattctccgc actcgatcaa aggacctcga cctctcccat cacacccatc
     1621 tcatgggcgt cgtcaacgtc acgccggact ctttctccga cggcggaaaa tatttcgacg
     1681 ccccggccgc cgtcgcccac gccgaagaac tgattcggga cggggcggac ctcctggacg
     1741 tgggggggga atcctcccgg cccggggccg atccggtccc tctggacgag gagctccgcc
     1801 gcgtgatccc cgtcattcag gagctcgccc gccgccaccc gtcggttccc ctctccatcg
     1861 acaccaccaa agccgaggtg gctcgccggg cgatgcgggc cggggcctcc ctcctcaacg
     1921 acatctcggc cctccggttc gaccccggca tggccgcggt cgcccgagaa acggacgtcc
     1981 ccgtcgtcct catgcacatg cagggcaatc cgcgcaccat gcagaccgcc ccttcctata
     2041 tagacgtcgt tcgggagatc ggttccttct tccgggaacg cctggccttt gccgaaaaaa
     2101 cgggaatcgc cgccgaccgg ctccttctgg accccggcat cggtttcggg aaaaccctcg
     2161 accacaacct gcagatccta aagaacctcc gcgcccttgc cgagatcgga cgaccgctgg
     2221 tgctcggcct gtcccggaaa accttcatcg cccgcctgct cgccgacggc gaaaagatcc
     2281 tgtcgccgca ggaacggtcc gaaggctccc tggccggcca tctctgggcg gcggcgcagg
     2341 gggcccacat ccttcgcgtg cacgacgtgc gcggaaccag acgggccctc cgcgtttggc
     2401 aggccattgc cagaggtctc gccgggtgat aaaatttgtc tcatctccgc cgcttttgat
     2461 ccacatattt ccctgagagg tcgcccatgt ttcgtcgaaa ctacatcgtc aaacccatgc
     2521 ttcaattgaa gtatctcctc tatggcctcg gcctggtcct cgtctccggc ctgtgcgtct
     2581 atttcatcat ggacctggcc atcgtccact cggaacggct ctcacagctg tcccagggag
     2641 agatcctgag cgtgcaggcg gccgcccgcg tggcgctcct ctgggccctg atcctcctgc
     2701 ttctcatcgt caccgtgggc accatcctca ttttccaccg cctcatcggg cccgtctacg
     2761 cggtggagaa agtcctccgc atggtggccg acggcgacct caccgtgtac atgcccctgc
     2821 ggaaaggcga cgaactccgc gaaatggcgg aggccgtgga acagatggtc ggcggaatcc
     2881 gacgccggct cctgcacgac aaggagaacg ccctggcctg ccagaccaaa ctccggcaga
     2941 tcgccgaggg caacccgtcc ctcaaggcgg aactcaacga catcgccgcc aagctggacc
     3001 gggtgggaca ggagttccgc ctcggctgat tcgtgaaaac acgaaatccg aaatacaaag
     3061 acgcgccgtc aatttcgtat ttcgtctttc gtgtttcgta ttcctaaact tctccgcgcc
     3121 tcccatcgaa aaatagcgtc atgagccgtt aaaaaatatg cctccatccc ccgttgtcag
     3181 cgatttcctc atcatcggca gcggcatcgc cgggctctct ctggcgctgc gggccgccca
     3241 atggggggac gtcgtcctcg tcaccaaact cgagatccag gaatccgcca ccgccaaggc
     3301 ccagggaggc ctggcggtgg tcgtctcccc ggaagattcc ttcgaacaac acatccgcga
     3361 caccctcgac gccggggccg gcctctgccg ggaggacgtg gttcggcacg tcgttgtcaa
     3421 cgccccggcg cgcgtccagg acctgatgca gtggggcatc cgcttctccg agaccgagac
     3481 ccacggcccc tgggaagaac ccaacttcgc cctgggattg gagggcggcc actcccgccg
     3541 gcgcatcctc cacgtgggcg actacaccgg gcgggccatc gaagaggcgc tggtggaacg
     3601 ggtgcgggag aacccgcgca tccgcgtctt tgaataccac gtcgccatcg acctcatcac
     3661 gcggcgacag ctggagaaaa aagatattcc ggcgcaggac aacgtctgcg gaggcgccta
     3721 cgtcctcaac gtcgagaccg gcgaaatcat ccccttcctg gctcgcggga cggccctctg
     3781 caccggcggc gccggaaaag tgtatctcta cacctccaat ccgaacatcg ccaccggcga
     3841 cggcatggcc atcgcctacc gcgccggagc caccctggcc aacatggagt tcgtccagtt
     3901 ccaccccacc tgcctttacc accccaaggc gaaaaatttc ctcatctccg aagccctccg
     3961 cggagagggc gccgtcctca aaaaccgcgc cggcgaacgt ttcgtgcaaa aagcgctccc
     4021 tcaggcggaa ctggccccgc gcgacctcgt cagccgggcc atcgacgccg aactcaagcg
     4081 gaccggcgac gactacgtct tcctcgacat cagccatcgc ggggcggact tcatccggcg
     4141 ccggttcccc agcatctacg aacgctgcgc cgccttcgac atcgacatca ccgcccagcc
     4201 catccccgtg gtgccggccg cccactactt ctgcggcggg gtcctcaccg acatccgggg
     4261 caacaccacc cttccgcgac tggccgcggt gggggaaacg gcctgcaccg gactccacgg
     4321 cgccaaccgc ctcgcctcca attccctccc ggaagccctg gtcttcgccc acgccgtggc
     4381 cgaaacctgg cagaccctgc gcgacagccc gccgcctttt ccggccggcg acgtgccgcc
     4441 gtgggtcttc gccctccacc ccacctcgga acgcgtggtg atcccccacg cctgggagga
     4501 gatccgccgg ttcatgtgga actacgtggg catcgctcgc acctcgctcc ggctggaccg
     4561 cgccctccaa cgcatcgtcc ttctggaaaa tgagatccgg gaacattacg atcacgctcc
     4621 cgtctccccc gacctgttgg aactccggaa catcgccgtc gtcgccgaca ccgtcatccg
     4681 ctgcgcgctg ggacgaaagg aatcccgcgg gctccactac aacctcgatt ttcccgcccc
     4741 ggacgacgcc ggaaagaaag actcgctgat ctccctttcc tccgacccgg ccggacacgc
     4801 ctgaccccca tggatgccga cgtcatccgc atccgcggcg cgcgggagca caacctcaag
     4861 aacatcaccc tcgatatccc ccgcaacaag ctggtggtca tcaccggcct gtccggatcc
     4921 ggcaaaagtt ccctcgcgtt caacaccatc tacgccgaag gccaacggcg ttacgtggag
     4981 agcctctccg cctacgcccg ccagttcctg gagttgatgg agaagccgga cgtggacctc
     5041 atcgaggggc tctctccggc gatcgccatc gaacaacgga cgccgtccca caacccccgc
     5101 tccaccgtcg gcaccgtcac ggagatctac gactatctcc ggctcctttt cgcgcgcatc
     5161 ggcgccccgc attgcccgcg ctgcgggcgg gagatccggc cccagtccgc ctcccaaatc
     5221 gtcacggagg ttctgaaaaa ccacgccggc cagaagatcc aggtcctggc ccccctcatc
     5281 agcggccgca tcggcaccta cgaggccctt ttcgcccggc tgcgcaagct gggctttgcg
     5341 cgcatgcgcg tggacggaca ggtcgcggac ctgcatcaaa agataaaact caaccgctac
     5401 aagaaacaca ccattgaggt ggtcgtggac cggctccagg tctcccccga aggccgggaa
     5461 cgcgtggtcg attccgtgga gaccgccctg cgcgaggcgc acgggctggt cctgcttctg
     5521 ccggaagggg acaagaccgg caagggccgc ctcttttcgg aacgccatgc ctgcgcccac
     5581 tgcgggctca gcctgccgga gatcgaaccc cgcctctttt ccttcaacag cccgtacggc
     5641 gcctgcgccg actgcgatgg actcggtgtc aaactggagg tggcggagga actgatcgtc
     5701 cccgacacct ccctttccgt ccgggacggg gcgctccagg catgggccaa ccccgtcacc
     5761 acgcgcacca accgatggaa aaaatcctgg gccggttact acgtcgagat cctggaaggc
     5821 gtctgccggc ggcatcacat ccccatggac aaaccctggc gccacatgtc ccgccaacat
     5881 caggacatcc tccttcacgg cggggtggac caccggtcgc cgtggtcgtc ctcggacaag
     5941 gagttcgagg gggtggtgcg gaatctcgag cggcgttatc gggaatcgga atccgatttc
     6001 gtgaaagagg ccatccgcga acgtttcatg cgcagccggc tctgcccgac ctgccgcggc
     6061 gcccgcctga aacccgaggc gctggccgtc tccgtggcgg aaaaatccat cgccgaggtg
     6121 gcgcggctgt ggggtgtgga cagcgtg
//
LOCUS       DAOTFD010000156         6056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9349_length_6056_cov_4.784203, whole genome shotgun sequence.
ACCESSION   DAOTFD010000156 DAOTFD010000000
VERSION     DAOTFD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9349_length_6056_cov_4.784203"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="P5079_08660"
     CDS             complement(<1..361)
                     /locus_tag="P5079_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30089.1"
                     /translation="MATFFKRKRRRTHTGPNKNEKKVLRGQEADRLVRTAGLMKDRFP
                     SVQRLTVQLDFVTPQQHLLDRQTRVFNPTDVCDFSVSCPGRCGGQGAFDLEAKIKSVI
                     DSRQARAEGSGTCAETLY"
     gene            complement(394..750)
                     /locus_tag="P5079_08665"
     CDS             complement(394..750)
                     /locus_tag="P5079_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY30090.1"
                     /translation="MGKKLYVGSLPYEITEDQLHAMFVAIAPVASVQLITDRNTGQSR
                     GFGFVEMATDEGAQAAIQKLNGSPSGSRTLVVNEARPQEKRGGGGGGRGHGGGGFGRG
                     GGGYGRGGGGGGRDRW"
     gene            1006..3390
                     /locus_tag="P5079_08670"
     CDS             1006..3390
                     /locus_tag="P5079_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRY30091.1"
                     /translation="MKKKPVAPKNPLGPEELRKMDAYWRAANYLSVGQIYLYDNPLLR
                     QPLRTEHIKPRLLGHWGTTPGLNFLYVHLNRLIKAHDLNMIFITGPGHGGPALIANTY
                     LEGTYSEYYPNVSRDADGMKQLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALAHAY
                     GAVFDNPDLLAACAVGDGEAETGPLATSWHSNKFLNPARDGAVLPILHLNGYKIANPT
                     VLSRLRRDELKSLFIGYGYTPHFVEGEDPKTLHRLMAATLDKAYAEIAAIQRAARGKK
                     SVSRPAWPMIVFRTPKGWTGPKEVDGKKTEGSWRSHQVPFSDLAQNPQHVKLLESWMK
                     GYKPEDLFDKDGKLRPDLAALAPKGIRRMGDNPHANGGILMKDLRMPDFRNYAVPVPR
                     PGHTEAEATRVLGTFLRDVMKLNRENNNFRVFGPDETASNRLGALFEATDKAWMADVL
                     REDDHLSPDGRVMEVLSEHLCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWL
                     KTTSKEIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKADVVRVYLPPD
                     ANTLLSVADHCLRSRNYINVIVAGKQPAPQFLDMGSAIKHCTNGIGIWEWAGTDDGGE
                     PDLVMACAGDVPTLETLAAVDMLRREFPDLKIRVVNVVDLMTLQPQSEHPHGLSDKDF
                     DALFTADKPIIFAYHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTSTPFDMVVRNDLD
                     RFHLVGDVVDRVPKLQRAAAHVKQTLRDKLIEHKEYIVKHGEDLPAIRDWKWPY"
     gene            complement(3478..4044)
                     /locus_tag="P5079_08675"
     CDS             complement(3478..4044)
                     /locus_tag="P5079_08675"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRY30092.1"
                     /translation="MLTDILQDVKAIFRNDPAARNFLETLICHTPLHAILIYRLAHFL
                     YGWRIPVVPRLLATLAKVWSGVEIHPAAAIGAGFFIDHGVGVVIGETAEIGVNCVMFH
                     NVTLGGTGKHSGKRHPSVKDNVLIGTNSVLLGPLTVGENSRVGANSFIIMHDVPPNCT
                     VGGTPAHILKRDGRRVDEPLPRTQFPAD"
     gene            complement(4095..5843)
                     /gene="ilvD"
                     /locus_tag="P5079_08680"
     CDS             complement(4095..5843)
                     /gene="ilvD"
                     /locus_tag="P5079_08680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRY30093.1"
                     /translation="MSKQAQGDINPGIDAVRRQGSRIVGEGMDKLPAAGLMKAAGVIG
                     SIEDRRKPFVTVINSYTTHIPGHAHLEQLGHLLERELKTLGYNVWYANVGGAVCDGVA
                     MGHFGMKYSLASRELITDQIETIVGAHPCDGWIGLGNCDKIVPGMYNAMVRLNVPAVY
                     VSGGPMLAGRDGTDLISIFEGVGRWAVKKISEGELAALADRACPGCGSCSGMFTANSM
                     NCLGEVIGLAVPGNGTVTAEVWADAEKTRTKLNPAREDLMRISARALKRCIEGNIRPR
                     DIVTKTALDNAFICDMAMGGSTNTVLHTLALASEAGIRYDLKRISALSARTPCLCKVS
                     PSRPEVHMEDVHAVGGIPAILKEVSKTGAGLDLSVPTVSGTLGDAVRTAPGPDGNVIR
                     KAAAPFSRTGGLVVFFGNLAPRGAVLKAAGVDPDMMRFEGPAVIFESQEEAFSGIING
                     KVKDGDVVVIRYEGPRGGPGMQEMLSPTAAIVGAGLKVALITDGRFSGGTRGLCIGHV
                     SPEAAAGGPVAALKKGDIIRIDAEKGRMDVLVKKAELARRLKKRKPFKSKIRHGWLAR
                     YAMHVTSADTGGVFNT"
BASE COUNT         1129 a         2032 c         1824 g         1071 t
ORIGIN      
        1 gatagagcgt ctcggcgcaa gtgccgctgc cttcggcgcg ggcctggcgg gaatcgatga
       61 cggattttat cttggcctcc aggtcgaaag ccccctgtcc cccgcaacgg ccgggacacg
      121 acaccgagaa atcgcagaca tcggtcggat tgaacacacg cgtctggcgg tccagcaggt
      181 gttgttgcgg cgtgacgaaa tccaactgaa ccgtcaaccg ttggacggaa gggaaacggt
      241 ctttcatcaa tccggcggtg cgcaccaagc ggtccgcctc ctgcccgcgc aggacttttt
      301 tttcgttctt gttgggtccg gtatgggttc tgcgtctttt tcgtttaaaa aacgtcgcca
      361 tattctatcg gacgatcctc ctttcgccgt caatcaccaa cggtcgcgtc cgccgcctcc
      421 accgccgcgg ccatatcccc cgccgccccg gccgaaacct ccgccgccgt ggcctcgccc
      481 gcctccgccg ccgccccgtt tttcctgggg acgggcctcg ttcaccacca gcgtgcggga
      541 acccgacgga ctgccgttca atttttggat cgccgcctgc gcgccctcgt ccgtcgccat
      601 ttcgacgaag ccgaacccgc gggattggcc ggtattgcgg tcagtgatca actgaacgct
      661 ggccaccgga gcaatcgcca cgaacatggc gtgcaactga tcctccgtta tttcgtaggg
      721 caaactgccc acatacaatt tctttcccat ttcacattct ccttaagtaa gtggcctgaa
      781 attaaaaagc cgcaaaggcc aatacctttt gcggcggtgt cggcttgaat tgcgcttcta
      841 tcaatatccc atattcactc cggagtgtca atatctattc cccccgccgg gcccgacggt
      901 gcaaaagcgg agaggaactc cgcggcccgg gacgggctcc ggaacaattc ccagccgttt
      961 cccgttaagg taaaataggg aggaaacccg acaaggagaa accgcatgaa aaagaaaccg
     1021 gtcgccccga agaacccgct gggccccgag gaattgcgca aaatggacgc gtattggagg
     1081 gccgccaact atctgtccgt cggccagatc tacctctacg acaaccccct cctccgccaa
     1141 cccctgcgga cggaacacat caagccgcgc ctgctcggcc attggggcac gacgcccggc
     1201 ttgaacttcc tctacgtgca cctgaaccgc ctcatcaagg cgcacgacct caacatgatc
     1261 ttcatcaccg gccccggcca cggcgggccc gcgctcatcg ccaacaccta cctggagggg
     1321 acctacagcg aatattatcc gaacgtctcg cgggacgccg acgggatgaa acaactcttc
     1381 aaacaattct cctttcccgg agggattccc agccacgtgg cgccggaaac gcccggttcc
     1441 atccatgaag gcggagaact gggatacgcg ctcgcccacg cctacggggc ggtgttcgat
     1501 aaccccgacc tcttggcggc ctgtgcggtg ggcgacggcg aagcggaaac cggtccgctg
     1561 gccaccagtt ggcattccaa caagttcctc aaccccgccc gcgacggcgc ggtccttccc
     1621 atcctgcacc tgaacggata caagatcgcc aaccccacgg tgctgtcccg cctgcgacgg
     1681 gacgaattga agagtctttt cataggctac gggtacaccc cccatttcgt cgagggggag
     1741 gacccgaaga ccctgcaccg gctcatggcg gccacgctcg acaaggcgta cgccgagatc
     1801 gcggccatcc agcgcgccgc ccgagggaaa aaatccgtat cccgtccggc ctggcccatg
     1861 atcgtcttcc gcacccccaa gggctggacg ggaccgaagg aggtggacgg aaaaaagacc
     1921 gaggggtcct ggcgttctca tcaagtcccg ttttccgacc tggcccaaaa ccctcaacac
     1981 gtcaaactct tggaatcctg gatgaagggc tataagccgg aggacctttt cgacaaggac
     2041 ggaaaactcc ggcccgacct ggcggcgctg gcccccaagg gaatccgacg catgggagac
     2101 aacccccacg ccaacggcgg catcctgatg aaggacctgc gcatgccgga tttccggaac
     2161 tacgcggtgc cggtgccccg gcccggacac accgaggccg aggcgacgcg cgtgctgggg
     2221 actttcttgc gcgacgtgat gaagctgaac cgggagaaca acaatttccg cgtgttcggg
     2281 cccgacgaga ccgcctccaa tcggctgggc gccctcttcg aagcgaccga caaggcctgg
     2341 atggccgacg tcctccggga ggacgaccac ctctcccccg acggccgcgt gatggaggtc
     2401 ttgagcgaac acctctgcca aggttggttg gaagggtatc ttctcacggg ccggcacggg
     2461 ttcttctcct gctacgaggc gttcatccac atcgtcgatt ccatgttcaa ccagcacgcc
     2521 aaatggttga agaccacgtc caaggaaatc ccttggcgaa ggcccatcgc ttcgctgaac
     2581 tatctgctca cctcccacgt ctggcggcag gaccacaacg ggttcagcca tcaggacccc
     2641 ggcttcatcg accacgtggt gaacaaaaaa gcggacgtgg tgcgcgtcta tcttccgccc
     2701 gacgccaaca ccctgctctc cgtggccgac cactgcctgc gcagccggaa ctacatcaac
     2761 gtgatcgtgg cgggaaaaca gccggcgcct caattcctgg acatgggctc ggccatcaaa
     2821 cactgcacca acggcatcgg catctgggaa tgggccggca ccgacgacgg cggcgagccg
     2881 gacctggtga tggcctgcgc gggcgacgtg cccacactgg agaccctggc ggccgtggac
     2941 atgctgcggc gggagttccc cgacctgaag atccgcgtgg tgaacgtggt ggacctgatg
     3001 acgctgcagc cccagagcga acatccccac gggctttccg acaaggactt cgacgccctt
     3061 ttcaccgcgg acaagcccat catcttcgcc taccacggct atccatggct catccaccga
     3121 ctcacctacc gccgaaccaa ccacaagaac ctgcacgtgc gcggctacaa ggaggagggc
     3181 accacctcca cgccgttcga catggtggtg cgcaacgacc tcgaccgctt ccacctcgtt
     3241 ggggacgtgg tggaccgcgt gccgaaactc cagcgggccg ccgcgcacgt caaacagacg
     3301 ctccgcgaca aactcatcga acacaaggaa tacatcgtca aacacggcga ggatcttccc
     3361 gccatccgcg actggaaatg gccttactga agcggttcgg aaacccgccg aaatagaacg
     3421 atccaccggt cctctcgaac cgacggcgga ttcccaaacc tcgtctgaac ggggagtcta
     3481 gtccgcgggg aactgcgtgc gggggagcgg ttcgtccacg cgacggccgt cccgtttgag
     3541 gatgtgcgcc ggcgtgccgc ccacggtgca gttgggcggg acgtcgtgca tgatgatgaa
     3601 ggagttggcg cccacgcggg agttctcccc caccgtgagc gggccgagca ggacgctgtt
     3661 ggtgccgatg aggacgttgt ctttgacgga ggggtggcgt ttgccgctgt gtttgccggt
     3721 gccgcccagt gtcacgttgt ggaacatgac gcagttgacg ccgatctcgg ccgtttcgcc
     3781 gatcaccacg cccacgccgt gatcgatgaa gaaccccgcg ccgatggcgg cggccggatg
     3841 gatctccacg ccggaccaga ccttcgccag ggtggcgagc aaccgcggca ccacggggat
     3901 gcgccacccg tagaggaaat gggccagccg atagatgagg atggcgtgca ggggcgtgtg
     3961 gcagatcagc gtctccagga aattcctggc cgccggatcg ttccggaaga tcgccttcac
     4021 gtcctgcagg atgtctgtca gcatgggtcc cctttcctca ggcagtataa cacatcaacg
     4081 catcggaggg acggttacgt gttgaacacg ccgccggtgt cggcgctggt gacgtgcatg
     4141 gcgtaccttg ccagccagcc gtggcggatc ttggacttga agggcttccg cttcttgagc
     4201 cgccgcgcca gttccgcttt tttcaccagc acgtccatcc ggcctttttc cgcgtcgatg
     4261 cggatgatgt cccccttctt cagggccgcc accggcccgc ccgccgccgc ctccggcgac
     4321 acatggccga tgcacaagcc gcgcgtgccg ccggaaaaac ggccgtccgt gatgagggcc
     4381 accttcaggc ccgcgcccac gatggccgcg gtggggctca gcatctcctg catacccggc
     4441 ccgccgcgcg gtccttcgta acggatcacc accacgtcgc cgtccttcac cttgccgttg
     4501 atgatgccgg agaaggcctc ctcctgcgac tcgaaaatga ccgcgggccc ctcgaaacgc
     4561 atcatgtccg ggtccacccc ggccgctttc agcaccgctc cccgcggggc caggttgccg
     4621 aagaacacga ccagtccgcc cgtccggctg aacggggcgg cggctttccg gatcacgttg
     4681 ccgtcggggc cgggggcggt gcgcaccgca tctcccagcg tccccgaaac cgtggggacg
     4741 ctcaaatcca gaccggcccc ggtctttgag acttccttca ggatggccgg gatgccaccg
     4801 acggcgtgca cgtcctccat gtgcacctcc ggacgggacg ggctcacctt gcagaggcac
     4861 ggcgtgcgcg ccgagagcgc gctgatccgt ttgaggtcgt agcggatccc cgcctcggaa
     4921 gccagcgcca gcgtgtgcag gacggtgttg gtggagccgc ccatggccat gtcgcagatg
     4981 aaggcgttgt cgagcgccgt tttggtgacg atgtcgcgcg gacggatgtt cccttcgatg
     5041 caacgtttca gggcccgcgc ggaaatccgc atcagatcct cccgggccgg gttgagtttc
     5101 gtacgggtct tctccgcgtc ggcccacacc tcggcggtga cggtgccgtt gccgggaacg
     5161 gccagcccga tcacctcgcc caggcagttc atggaattgg cggtgaacat acccgagcag
     5221 gaaccgcagc cggggcaggc ccggtccgcg agcgcggcga gctccccttc ggatatcttc
     5281 ttcaccgccc atctgccgac gccctcgaag atggagatga ggtcggtgcc gtcgcggccg
     5341 gccagcatgg ggccgcccga cacgtacacc gccggcacgt tgaggcgcac catggcgttg
     5401 tacatgcccg gcacgatctt gtcgcagttt ccgagcccga tccagccgtc gcagggatgc
     5461 gccccgacga tggtctcgat ctggtcggtg atgagctcgc ggctggcgag ggaatatttc
     5521 atgccgaaat ggcccatggc cacgccgtcg cacaccgcgc cgccgacgtt ggcgtaccac
     5581 acgttgtacc ccaaggtctt cagttcccgc tccagcaggt gccccagctg ttccaggtgg
     5641 gcgtggccgg ggatgtgggt ggtgtaggag ttgatcacgg tgacgaaagg tttccggcgg
     5701 tcttcgatgg acccgatgac gccggcggcc ttcatgaggc cggcggcggg cagtttgtcc
     5761 atcccctccc ccacgatgcg actgccctgc cgccgcaccg cgtcgatgcc gggatttata
     5821 tcaccttgcg cctgtttgga catgggattt tctcctgagt gtggtcgttc cagagattct
     5881 aggccgaagc ggcggtgttc ttggggaaca cgccccgctt ggctttggcg agaacgtcgc
     5941 ggaaatccga cgggaaatcg ttcttctgcg acagccagtc gatataggac ggatcgtggc
     6001 ggcagacttc cgtcaaggcc cgtccgcggt acttgccgaa gctgaagaag gcctcc
//
LOCUS       DAOTFD010000157         6025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9416_length_6025_cov_3.907538, whole genome shotgun sequence.
ACCESSION   DAOTFD010000157 DAOTFD010000000
VERSION     DAOTFD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9416_length_6025_cov_3.907538"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="P5079_08685"
     CDS             complement(<1..402)
                     /locus_tag="P5079_08685"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRY30094.1"
                     /translation="MNVAAWPFRGGAAWRGVFFLPSLLYGAAVRLRSSAYAAGFLETV
                     RAGVPVFCVGNMTTGGTGKTPVVIWLASFLRQKGRRPAVLSRGYGRRSKGNVIVSRGQ
                     GPLATVQDSGDEPFLIASLVDGLAVLVGSRRV"
     gene            complement(402..1418)
                     /locus_tag="P5079_08690"
     CDS             complement(402..1418)
                     /locus_tag="P5079_08690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRY30095.1"
                     /translation="MKILVIQLRRIGDVIVTLPVVSALRAQFPQARIDFLVEPAAAPV
                     VRGFPGVDDALVFEKRHFWRWAWDIRRRGYDWVLDFMNNPRTAQIALLSGAPVRAGFE
                     APLWGRVYTHRLPRPSVPQYAVQSKFDLLRALGLTPSVPALPEIRVTEEDFSPVGGWW
                     KEKSMDAYAERVAVMPAHRRPIRRWPAHKFSELIRLLNGKKDRAVILFGGPGEEGYIA
                     GLRAPFSERVFAAPVAGLRQAAALLSRCHVAVTNCSGLMHLAVAVGTPTLTVYGPTWP
                     ESWNPRVRPHRYVQTSGLRCIGCNRDECPYAHECLEWISAERVCGETESMLKERRAVP
                     AGGR"
     gene            complement(1435..2232)
                     /locus_tag="P5079_08695"
     CDS             complement(1435..2232)
                     /locus_tag="P5079_08695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY30096.1"
                     /translation="MIPAGFWWGAAALSALGVSARWNWWRPAAEGLPVLMYHKVGDPP
                     PGSRLKKLWVSVAKFRRQMEYLKTHGYQPATFAELAEDPARFSRPVVLSFDDGYLNNY
                     QNAFPLLREFGFRAVVFLVVDAIGKDNFWHDPRNEVRIPMLSREQILEMQAAGVEFGS
                     HTFSHPRLSRLDDAAARREIIESRRALEVLLGRAPVPFAYPYGDGQDEPRLWKIVEEA
                     GYRWACAIHQGKARPEKNPYSLRRIFVRGDDGLWDFHLNLTRGRARF"
     gene            complement(2229..2987)
                     /locus_tag="P5079_08700"
     CDS             complement(2229..2987)
                     /locus_tag="P5079_08700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30097.1"
                     /translation="MPRLSVILITHNEEKDLPRCLDSLKGVADEIVVVDSHSADRTRD
                     IARKYTDKVFERDFAGYGPQKQFALEQASGEWILNLDADECLTPGLAMEIRTYFQKGD
                     PVPNGFWIPYENYFLGRRLRFGGSFREHHLRLFRKSQGHYAGRVIHEGIAVSPPLGYL
                     NGRIRHESYRGFTEYLEKCNEYTRLIAGQKFAQGRRFAAWHHLRLPWEFFVRYVVKLG
                     FLDGQAGFVYAVLSAYYAWLKHVRLLDYERGEGI"
     gene            complement(2998..4104)
                     /locus_tag="P5079_08705"
     CDS             complement(2998..4104)
                     /locus_tag="P5079_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30098.1"
                     /translation="MERPRVLHLNTERTWRGGERQLFFLAKELQRRGYEQRVACRPGF
                     PLERFCRQAGLSVVPVSPWGEFDLWAAGRLRRAMRDEKIDILHAHTGHAVGLGALALR
                     RGGPSFIATRRVDFPLKKNFLSRWKYRRVDRLVAISGKVRDVLTEGGMDPARIPVVHS
                     GIEGADYPSAGDKARLRKEKGLPVEGIFVTQVAALVPHKDHATLLAAARRVCDKLPSL
                     RFFLLGDGELRSGLERKTRELGLSDNVVFLGHRMDALDYIALADAFVLSSVEEGLGTS
                     LLDALFIGVPVVATRAGGIPEIFGSPSAPELVPARAPDELADQLVRVLSDRAYAEQRV
                     TRGREIVKKFSVSAMADAYEKMYKELSRRKYGIT"
     gene            complement(4107..5168)
                     /locus_tag="P5079_08710"
     CDS             complement(4107..5168)
                     /locus_tag="P5079_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY30099.1"
                     /translation="MNILHVLDEPWDSALTDYALTLGSALKVRGHNVHFACRAGSYAL
                     REAEKRGFSRAILGSFLDFRRLLRRRDIELVNAHTGNGHAWAWWGALGRAMALVRTRG
                     DARPLKKNPAHSLIYRRTDAVIGASQCISAQYRSLFGGLADRLWTVYPGLDIPEGTEE
                     PDGPLRIALVGRLDPVKGQMHFLEAVQQLRPQLRDETFLIVGEEKNTPLEELKRLAEH
                     YHVSRWVSFAGRQTDIRSFMKTCHVGVIASVGSESLSRVCLEWMSVGRPVVATAVGCL
                     PELVSTGENGFLVPPGSPQAMASCFLTLIRQKGFRQDLGRRAHAEAKSRFGLDRFAAE
                     TEKVYEAARRHRWPVKGKN"
     gene            complement(5165..>6025)
                     /locus_tag="P5079_08715"
     CDS             complement(5165..>6025)
                     /locus_tag="P5079_08715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRY30100.1"
                     /translation="FWGWCREIRRRSFDVYVDLHDTLRSRLWGIFSGVPVRVRYDKRA
                     WPRRLLVWFKKKGPRPDGRVCDWYLESLGKMDVPVRSRRPELRFSEREGLGAAWKERL
                     GDGPIIGIAPGARHATKRWLPERFAAAADELAEKWPMLTGESQTPRVLILGGADEREA
                     AEKVLARLRAPALDTTGRTTVGELLGLVRRCALVLSNDSGVMHAAYAQHVPVVAVFGP
                     TVREFGFFPEPDCSEVLERGGLYCRPCSLHGGEGCPEGHFSCMRDLRAEDAVAAAKRL
                     LQRTRRTSAT"
BASE COUNT         1189 a         2087 c         1786 g          963 t
ORIGIN      
        1 cacccgacgg ctgcccacca agacagccag cccatcaacc aggctagcga tcaagaaggg
       61 ctcatcacca ctgtcctgaa ctgtggcaag aggaccttgc ccgcgactga caatgacatt
      121 tcctttactg cggcgcccat agccgcggct gagcacggcg ggtcggcggc ccttttggcg
      181 gaggaaagac gccagccaga tgacgaccgg ggtcttcccg gtgccgcccg tcgtcatgtt
      241 gccgacgcaa aagaccggga cgcccgcccg gacggtttcc agaaaaccgg ccgcgtaagc
      301 cgacgaacgc aaacgcaccg ccgctccata caaaagggac ggaaggaaaa agacgccccg
      361 ccacgccgcc ccgccgcgga acggccacgc agcgacgttc atcaccgacc gcccgccgga
      421 acggcgcggc gttccttcag catggattcc gtctccccgc agacccgttc cgccgagatc
      481 cattcgaggc actcatgcgc atagggacac tcatcgcggt tgcatccgat gcaccggaga
      541 ccgctggtct gcacgtatcg atggggccgg acccgggggt tccaggattc cggccaagtg
      601 gggccataga ccgtcaaggt cggcgttccc accgccacgg ccagatgcat caacccgctg
      661 cagttggtga ccgccacatg gcaccgggaa aggagcgccg ccgcctggcg cagaccggcc
      721 accggcgccg cgaacacccg ttccgagaag ggggcgcgca ggccggcgat atatccctcc
      781 tcccccggcc ccccgaacaa gatcaccgcc cggtcttttt tcccgttcag caaccggatc
      841 aactccgaga acttatgggc cggccagcgg cggatgggcc ggcggtgggc cggcatgacg
      901 gcgacccttt ccgcataggc gtccatggat ttttctttcc accagcctcc gacgggagag
      961 aagtcctcct ccgtgacgcg gatctccggc agcgccggga cggacggggt cagtcccagg
     1021 gcccgcagga gatcgaactt gctttggacg gcgtattgag gaacggacgg gcgcgggaga
     1081 cgatgcgtat acacccggcc ccagagcggc gcctcgaaac ccgcccgaac cggggccccg
     1141 ctgagcaggg cgatttgcgc cgtccggggg ttgttcatga aatccagcac ccaatcgtag
     1201 ccccggcggc ggatgtccca cgcccacctc caaaaatgcc gtttctcgaa gaccagggcg
     1261 tcgtccactc ccgggaatcc cctcacgacc ggggcggccg cgggctcgac gaggaaatcg
     1321 atccgggcct gcgggaactg cgcccgcagg gcggagacca cgggaagagt gacgatgaca
     1381 tcgccgatgc ggcgaagttg gatgacgagg attttcatga gacgccgggc ggggtcagaa
     1441 ccgggcgcgg ccccgggtca gattcagatg gaaatcccaa aggccgtcgt ctccccggac
     1501 aaaaatacgg cggagcgaat aggggttctt ttccggccgc gccttgccct gatggatggc
     1561 gcaggcccag cggtagccgg cctcttccac gatcttccag agacggggct cgtcctgccc
     1621 gtcgccgtag ggataagcga agggcaccgg cgcgcggccc aaaaggacct ccaacgcccg
     1681 ccggctctcg atgatttccc ggcgggccgc cgcgtcgtcc aaccgcgaaa ggcgcgggtg
     1741 gctgaacgta tgagacccga actccacacc ggccgcctgc atctcgagta tctgctcccg
     1801 cgaaagcatg ggaatgcgca cctcgttgcg gggatcgtgc caaaaattgt ctttgccgat
     1861 ggcgtcgacg acgagaaaaa cgaccgcgcg gaaaccgaat tccctcagga ggggaaaggc
     1921 gttctgataa ttgttcaagt aaccgtcgtc gaacgagagg acgacgggcc gggaaaaacg
     1981 ggcgggatcc tcggccaatt cggcgaacgt cgccggctga tagccgtgcg ttttcagata
     2041 ttccatctgc cggcggaact tcgccacgga cacccacaat ttcttcagcc gggatcccgg
     2101 cggcgggtcc ccgactttgt ggtacatcag cacgggaaga ccttcggccg ccggccgcca
     2161 ccaattccaa cgcgccgaaa cgccgagggc ggagagcgcc gccgcgcccc accaaaaacc
     2221 ggcggggatc atatcccctc ccccctctcg taatccagca gccgcacgtg tttcaaccag
     2281 gcgtaatacg cgctcaacac cgcgtagacg aacccggcct gtccgtccaa aaagcccagc
     2341 ttcaccacat aacggacgaa aaattcccag ggcaggcgga ggtggtgcca ggcggcgaac
     2401 cggcgcccct gggcgaattt ctgtccggcg atgagccggg tatactcgtt gcatttttcc
     2461 agatattcgg tgaacccccg ataactctca tgccggatcc ttccgttcaa atagccgagg
     2521 ggcggactca cggcgatgcc ctcatggatc acccggccgg cgtaatgtcc ctgcgatttc
     2581 cggaaaagcc tcaggtgatg ttcgcgaaac gagccgccga accgcaaccg gcggcccagg
     2641 aaataattct cgtaggggat ccaaaaaccg ttggggacgg ggtccccttt ctgaaaatac
     2701 gtgcggattt ccatggccag tccgggcgtc aggcattcgt ccgcatccag gttgagtatc
     2761 cattcgcccg acgcctgttc cagagcgaat tgcttttgcg ggccgtaccc ggcgaaatcc
     2821 ctttcgaaca ccttgtcggt gtatttacgg gcgatgtccc gcgtccggtc ggcggagtgg
     2881 ctgtccacga cgacgatctc gtccgccacg cccttcaggc tgtccaggca tcggggaaga
     2941 tccttttctt cgttatgggt gatgaggatg acgctcaaac gcggcataaa ccgtccctca
     3001 cgttattccg tatttccttc gtgaaagttc tttatacatt ttctcataag cgtcggccat
     3061 agcggaaacg gagaattttt tcacgatctc ccggccgcgc gtgacccgtt gttcggcata
     3121 ggccctgtcg ctcaaaactc tcacaagctg atcggccaac tcgtccggcg cccgggcggg
     3181 caccagctcc ggggccgacg gcgaaccgaa gatttcgggg atgcccccgg cccgggtcgc
     3241 caccaccggc acaccgatga acagcgcgtc cagcaacgac gtgccgaggc cctcctccac
     3301 ggaactcaac acgaacgcgt cggcaagggc gatatagtcg agcgcatcca ttcgatgtcc
     3361 taaaaacacc acgttgtccg agagacccag ttccctcgtt ttccgttcaa gtccggaccg
     3421 gagttccccg tcgcccagaa gaaaaaaccg gagggacggc agtttgtcgc agacacggcg
     3481 ggcggcggcc agcaacgtgg cgtggtcctt gtgcgggacc aacgccgcca cctgggtgac
     3541 gaaaatccct tccaccggaa gtcccttttc cttccggagt cgcgccttat cgccggccga
     3601 cggataatcc gccccctcga tcccgctgtg gacgaccggg atccgggccg ggtccatccc
     3661 gccttccgtc aaaacgtccc tcaccttgcc ggaaatcgcc accaggcggt ccacccggcg
     3721 atacttccag cgactgagga aattcttttt caacggaaaa tccacccggc gggtggcgat
     3781 aaaagacggg ccgccgcgcc gcagagccag cgctcccaat cccacggcgt gtcccgtgtg
     3841 cgcgtggaga atgtctattt tctcgtccct catcgctctc cgcagccgcc ccgccgccca
     3901 caggtcgaac tccccccacg gcgaaaccgg caccacggac aaaccggcct gacggcaaaa
     3961 acgttccagc gggaaccccg gccggcaggc cacccgctgt tcatagccgc ggcgttgaag
     4021 ttccttcgcc agaaaaaaga gctgccgttc ccctccccgc caggtgcgtt cggtattcaa
     4081 atgcaggacc cgcgggcgct ccatcgtcag ttcttcccct tcaccggcca ccgatgccgc
     4141 cgggcggcct cgtagacctt ttccgtttcc gcggcaaagc ggtccaggcc gaaacggctc
     4201 ttggcctcgg cgtgcgcccg acggcccaga tcctgccgga accccttctg ccggatcaac
     4261 gtcagaaaac agctcgccat ggcctgcggg ctgccgggcg gaacgagaaa accgttctct
     4321 cccgtggaaa ccagttccgg cagacagccc accgcggtgg cgacgacggg ccgcccgacg
     4381 gacatccatt ccaggcacac ccgcgagaga gactcgctcc ccaccgacgc gatgacgccg
     4441 acgtggcagg ttttcatgaa cgaccggata tccgtctgtc tccccgcgaa agagacccat
     4501 cgggaaacat ggtaatgttc cgccaacctc ttcagctctt ccagcggcgt gttcttctcc
     4561 tcacccacga tcaaaaaagt ctcgtccctc agttgcggcc gcaactgttg aaccgcctca
     4621 aggaaatgca tctgtccctt gaccggatcg agccgcccca ccagggcgat gcgcaagggg
     4681 ccgtccggct cttccgtccc ctccggaata tccagaccgg gatagacggt ccaaagacgg
     4741 tcggcgagcc cgccgaagag cgaacggtat tgggcggaaa tgcactgaga ggccccgatg
     4801 acggcgtcgg tccggcgata gataagcgaa tgcgccggat ttttcttcaa gggacgggca
     4861 tcgccgcgcg tgcggaccag ggccatcgcc cgtcccaaag caccccacca cgcccacgca
     4921 tggccgttcc ccgtgtgcgc gttcacgagc tcgatgtccc gtcgacggag cagtctcctg
     4981 aaatccaaaa aagagccgag gatcgcgcgg gaaaatcctc ttttttccgc ctcacgcaga
     5041 gcgtagctgc cggcgcggca ggcgaaatgg acgttgtggc cgcgcacttt cagggccgag
     5101 ccgagggtga gcgcatagtc ggtgagggcg gaatcccacg gttcgtctaa aacgtgcagg
     5161 atattcatgt cgccgacgtc cgccgcgtcc gctgcaacaa ccgtttcgcg gccgcgaccg
     5221 cgtcctccgc gcggaggtcc ctcatgcagg aaaaatggcc ttcggggcat ccctcgccgc
     5281 cgtgcaggga gcacggccgg cagtaaagcc ccccccgctc cagcacctcg gaacaatccg
     5341 gttccggaaa gaaaccgaat tcccgaacgg tggggccgaa aaccgccacc accggaacgt
     5401 gctgcgcgta ggcggcgtgc atcacgccgg aatcgttgga caacaccagc gcgcaccggc
     5461 gcaccagccc gagcagttct cccaccgtcg tccggcccgt cgtgtcgagc gccggcgcgc
     5521 gcaaccgggc caggaccttc tccgccgcct cgcgctcgtc cgccccgccc aggatcaaga
     5581 cccggggcgt ctgggattcc ccggtcaaca tcggccactt ttccgccagc tcatccgcgg
     5641 cggcggcaaa acgttccggc agccaacgct tggtcgcgtg tcgggccccc ggcgcgatgc
     5701 cgatgatcgg accgtcgccc agccgctcct tccacgcggc gcccagcccc tccctttccg
     5761 aaaagcggag ttccggacgg cggctccgaa cggggacatc cattttgccc agactttcca
     5821 ggtaccagtc gcagacccgg ccgtccgggc ggggcccctt cttcttgaac cacaccaaca
     5881 accgccgggg ccaggcgcgc ttatcgtagc ggacacggac ggggacaccg gaaaaaatcc
     5941 cccacagccg ggaacgcagg gtatcatgaa ggtccacata gacgtcgaac gagcggcggc
     6001 ggatctcgcg gcaccacccc caaaa
//
LOCUS       DAOTFD010000158         5992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9474_length_5992_cov_4.617484, whole genome shotgun sequence.
ACCESSION   DAOTFD010000158 DAOTFD010000000
VERSION     DAOTFD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9474_length_5992_cov_4.617484"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="P5079_08720"
     CDS             <1..523
                     /locus_tag="P5079_08720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY30101.1"
                     /translation="LMNKGRSASKESLFLWSADGVQRIRKRVRGFFSAPAVSSAVPPA
                     PAADPRRYPRLDLKLPILYRVLSGGVSRLPPEIQPCLLAQSTNVSPIGLCLDLAEEMA
                     PETVLALTIHVVDRREKFSAVGRVVWSKPSPQPGHFLTGLEFVVVKGENVKKEDHVRM
                     EALTEEYGSLIGT"
     gene            633..1763
                     /gene="bioB"
                     /locus_tag="P5079_08725"
     CDS             633..1763
                     /gene="bioB"
                     /locus_tag="P5079_08725"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRY30102.1"
                     /translation="MHSSRRSRPPRRPAKDFSALAVRLAEKSLRGSPLSRAEARRVLA
                     CTAQEMPALLSAVFRVRRAFFGRKVKLHFLANIQSGVCPEDCAYCSQSRVSKAPVERY
                     RLLPPRQILGAAENAVRWKASRLCLVASGRQFSQTDAVSLAGAIRTIKRRHPRLEICC
                     SLGFLPEPQARLLRDAGVEAYNHNLNTSERFYRSICSTHGYAERLETIARARAAGFHV
                     CSGAVFGMGETPDDVVRVAFHLRKLGVESIPVNFLIPVPGTPLEGRESLTPLLCLKIL
                     CLFRLVCPRSALRIAGGRELHLRSLQPLALFAANAMFVGDYLTTGGAAAQRDIEMIQD
                     MGFEILGGAPPEGKACVAGRVKVISSRKAFRWAGPSSAGAAS"
     gene            1760..2479
                     /locus_tag="P5079_08730"
     CDS             1760..2479
                     /locus_tag="P5079_08730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HRY30103.1"
                     /translation="MNVLVTGASRGIGERIARAFCKEGHRVLLNYHVDKERALRVRDA
                     LRAQGGEAEVCGGDVADPAAARALVKRTVELWGAIDGLVNNAGLCRDKTILKMTEEEW
                     RRVLDVNLSGTFWCLKFAAEEMARKKSGFIINIASLLALRGGVGCANYAAAKAGVVGL
                     TKSAARELGRFNVRVNAVLPGFHNTDMGAAAWAKNAEKILGEHVLGRLGDLDELGRFV
                     VFLSQQQSVSGQIFNFESRVV"
     gene            2496..3593
                     /locus_tag="P5079_08735"
     CDS             2496..3593
                     /locus_tag="P5079_08735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRY30104.1"
                     /translation="MDVQSVVVTGIGLMSPLGAGRDIGWKKLLDGVSGVRAGAAVCPL
                     SGSREPRAVQLALAAAEEAAREAGLPAGPRLGERWGCSVSASKPIVSEGLRWLPPEEV
                     PFAVARRFGVCGPVLNVSSACATGLHSVMTAAGWIREGRCDAVLAGSAESSLHPLYLA
                     GFSQMGVLSTRGTVRPFDRDRDGFVVGEGAAVFVLEREEPALRRGAEIYGRIAGWDFS
                     CDASHAVRFNSGGDRIAASLARALGRAGVSPRNVDYLNAHGTATLMNDELEVRALKAV
                     FGGASVPPVSSTKGATGHLLGATGAVELAFCLLALKTGRLPPTVNLSHPIAREFDFVP
                     NKGRPARLSTAVSLSFGFGGAIGAMVVQNPC"
     gene            3587..4765
                     /gene="bioF"
                     /locus_tag="P5079_08740"
     CDS             3587..4765
                     /gene="bioF"
                     /locus_tag="P5079_08740"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRY30105.1"
                     /translation="MLNLEKELKDLEDRNLYRVLRVMRSAPGARVTVEGEELLNFASN
                     NYLGLSADDRLKEAARSAIGEWGAGATASRLLGGNLELHERLERALADFKRTEACAVF
                     PSGYHANLGTLPALMGENDTVILDRLCHASLVDGARLSRARLRVFAHNDAVDLEKVLK
                     RAASSRGRRWIVTESVFSMDGDVAPLREIAALAARYGVQTYVDEAHATGVWGPDGRGL
                     VNELGLEKKLDVCMGTLSKALGGAGGFVCGRRDLVEWIHNRCRSFIYSTGLPPASAAT
                     ALKALEICLTEPDRRRRLFSSADRLRRGLSGFPRARSSSAAGIFESSRGPVVPFVIGS
                     EADALALSRSLWKKGIFVPAIRPPTVPKGTARLRFSVTAEHTEADVDLVLEAMRRHAG
                     "
     gene            4755..5468
                     /gene="bioD"
                     /locus_tag="P5079_08745"
     CDS             4755..5468
                     /gene="bioD"
                     /locus_tag="P5079_08745"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRY30106.1"
                     /translation="MRVSGRPSLGLSARSSGVFIIGTDTGVGKTMVGALLARSLREAG
                     VRVGVMKPYVSGGWGDVRALKSAAGLQEPLSVLAPAFFRYPLAPAASALRGFGPRPAA
                     WRGVLRAFRRLRRRFPFLVVEGIGGVLVPLEGRLTVGDLARRLKLPVWLVARPGLGTI
                     NHVLLSLEALRRRGVRVERVVLSGFSGRTAAEKTNPAMLRRLTDQKVFILPKVTANVR
                     RALVGRWSAEIKRKWKGAV"
     gene            5450..>5992
                     /locus_tag="P5079_08750"
     CDS             5450..>5992
                     /locus_tag="P5079_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY30107.1"
                     /translation="MEGRGLMARGNKADALAAWDKKYVWHPFTQQADWVKEEPVVVES
                     ARGWHFKDTRGRRYLDGVSSLWVNVHGHGEPALNAALRRQLKKMAHVTFLGLTHEPAV
                     RLARELVRLAPRGLTRVFYSDNGSTAVEVAMKMAFQYGAQREGHVRRSEFFALANSYH
                     GDTLGSVSVGGIGLFHKRFRP"
BASE COUNT          881 a         1783 c         2145 g         1183 t
ORIGIN      
        1 gctgatgaac aaggggcgtt ccgcctccaa agagagcctg ttcctttggt ctgccgacgg
       61 agtgcagcgg atccgtaaaa gagtgcgggg ctttttttcg gccccggcgg tttcttccgc
      121 tgttcctccg gcgccggccg ccgacccgcg tcgttacccg cggttggacc tgaaacttcc
      181 catcctttat agggttttga gcggcggggt ctcccgtctt cctcccgaga tccagccgtg
      241 cctgttggcc cagagcacca acgtgagccc catcgggctc tgcctggatt tggcggagga
      301 gatggccccg gagaccgtgt tggcgttgac catccacgtg gtggaccggc gggaaaagtt
      361 ctccgccgtc ggccgcgtgg tgtggtccaa accgtcccct cagccgggcc attttctgac
      421 cggcctggag ttcgtggtgg tgaaggggga aaacgtcaag aaagaggatc atgtgcggat
      481 ggaggcgctg accgaggaat acgggtccct gatcgggacc tgagtcgttg ccgacccttc
      541 tgggggacgt ttttgaatgt caaccgatga aaaataatac ggttgacatt ttttttggcc
      601 tgtagtaacg ttacttggaa atgcctgccg tcatgcactc atcccggaga agccgtcccc
      661 cccgccgccc ggcaaaggat ttttcggcac tggccgtccg gctggcggag aaaagcctgc
      721 gcggttcccc cctttcccgc gccgaggctc ggcgcgtcct cgcctgcacg gcccaggaga
      781 tgccggcgct tctgtccgcg gtgttccgcg tgcgccgcgc tttcttcggc cgtaaggtga
      841 aacttcattt cctggccaac atccagagcg gggtctgtcc ggaagactgc gcctactgct
      901 cccagtcccg cgtctccaag gcccccgtgg aacgataccg tcttttgccg ccccggcaga
      961 tcctcggggc ggcggagaac gccgtccgct ggaaggcctc tcggttgtgt ctggtggctt
     1021 ccggccggca gttttcccag accgacgccg tttctctggc cggagccatc cggaccatca
     1081 aaagacgtca tccgcgtttg gagatctgct gcagtctggg ttttttgccg gaaccgcagg
     1141 cgcggcttct ccgagacgcc ggcgtggagg cgtacaacca caacctgaac accagcgaac
     1201 ggttttatcg gagcatctgt tcgacgcacg gctacgccga gcgtttggag accatcgcac
     1261 gggcccgtgc cgcggggttc catgtctgca gcggcgcggt gttcggcatg ggggagacgc
     1321 ccgacgacgt cgtccgtgtc gctttccatc tcaggaaact cggcgtggag tccatcccgg
     1381 tgaacttcct gatcccggtg ccggggacgc ccctggaagg gcgagagagc ctgacgccgt
     1441 tgttgtgcct gaagatcctt tgtctgttcc ggctggtgtg tccccgatcg gcgttgcgca
     1501 tcgccggcgg ccgggagctc catctgcgga gccttcagcc gttggccctt ttcgccgcca
     1561 acgccatgtt cgtgggcgat tatctcacca ccggaggcgc ggccgcccag cgcgacatcg
     1621 agatgatcca agacatggga tttgagatct tgggaggggc gccgcccgaa ggaaaagcgt
     1681 gcgtggccgg gcgggtgaag gtgatttcct ctcgaaaagc gttccggtgg gccggtccct
     1741 cctcggcggg ggcggcctcg tgaacgtcct ggtgacgggc gcttcccgcg gcatcgggga
     1801 acgtatcgcg cgggcgtttt gcaaagaggg acaccgggtg ttgctgaact accatgtcga
     1861 caaggagcgg gcgctgcggg tgagggatgc cttgcgggcc cagggcgggg aggcggaggt
     1921 gtgcggcggg gacgtggccg atcccgccgc ggcccgcgcg ctggtgaagc gcacggtgga
     1981 actgtggggg gccatcgacg gtttggtgaa caacgccggc ctctgccggg acaagaccat
     2041 cctgaagatg acggaggagg agtggcgtcg ggtgctggac gtcaacttga gcggcacgtt
     2101 ctggtgtttg aaattcgcgg cggaagagat ggcgcggaag aagtccgggt tcatcatcaa
     2161 catcgcctct cttttggccc tgcgcggcgg ggtgggctgc gccaactacg cggcggccaa
     2221 ggccggggtg gtcgggctca ccaagtcggc ggcccgcgag ctgggccggt tcaacgtccg
     2281 cgtgaacgcc gtcctgccgg ggttccacaa cacggacatg ggcgcggcgg cctgggcgaa
     2341 gaacgcggaa aagatcctcg gggaacacgt gttgggccgc ctgggggatc tggacgaact
     2401 gggccggttc gtcgtttttc tttcgcaaca gcaatccgtc tccggacaga tcttcaattt
     2461 cgaatcccgc gtcgtttaaa tcccgactcg catttatgga cgtgcaatct gttgtggtga
     2521 cgggcatcgg cctcatgtcg ccgctcggcg ccggccggga catcgggtgg aagaagctgt
     2581 tggacggtgt ttccggcgtg cgggccgggg cggccgtttg tcccttgagc ggctcccggg
     2641 agccgcgcgc ggtacagttg gcgttggcgg cggcggagga ggcggcgcgc gaggccggcc
     2701 tcccggccgg tccgcggctc ggggagcggt ggggatgttc ggtcagcgcc agtaaaccca
     2761 ttgtttcaga ggggctccga tggcttccgc cggaagaggt ccctttcgcc gtggcccggc
     2821 ggttcggtgt gtgtgggccg gtgttgaacg tgtcctccgc ctgcgccacg gggctccatt
     2881 cggtcatgac cgcggcggga tggatccgcg aggggcgctg tgacgcggtg ttggccggtt
     2941 cggcggaatc ctctttgcac ccgctgtatc tcgccggttt ttcccaaatg ggagtcctct
     3001 cgacccgggg gacggtgcgc cctttcgacc gggaccggga cgggttcgtg gtgggggaag
     3061 gcgcggcggt ctttgtcctg gagcgggagg aaccggcgtt gcgccgcggg gcggagatct
     3121 atggacggat cgccggatgg gatttttcct gcgacgcctc ccacgccgtg cggttcaatt
     3181 ccggcgggga ccggatcgcg gccagcctcg cgcgggcctt gggccgtgcc ggcgtgtccc
     3241 cccggaacgt ggattatctc aacgctcacg gcaccgcgac gctcatgaac gacgaattgg
     3301 aggtccgcgc gttgaaggcg gttttcggcg gcgcgtccgt gccccccgtc tcctccacaa
     3361 aaggggccac gggacatctg ttgggcgcga cgggcgccgt tgaactcgcg ttttgtcttt
     3421 tggcgcttaa aacaggacgt ctgccgccca ccgtgaacct ttcccatccg atcgcccggg
     3481 aatttgattt cgttccgaac aaaggccggc cggcccgcct ctcgaccgcg gtttcactct
     3541 ccttcgggtt cggcggtgcg atcggcgcga tggtggttca aaatccatgc tgaacctcga
     3601 aaaagaactg aaggacctcg aagacaggaa cctctatcgc gttttacgcg tgatgcgttc
     3661 ggccccgggc gcccgggtga cggtggaggg cgaggaactg ttgaatttcg cctccaacaa
     3721 ttatctcggt ttgtccgcgg acgaccgtct caaggaggcc gcccggtccg ccatcggcga
     3781 atggggggcg ggggccaccg cttcccggct tttggggggc aacctggagt tgcacgagcg
     3841 cctggagcgg gccctggccg atttcaagcg caccgaggcc tgtgcggtgt tcccctccgg
     3901 ctatcacgcc aacctcggca ccctcccggc gttgatgggg gaaaacgaca cggtgatcct
     3961 ggaccggctc tgtcacgcct cgctggtgga cggggcgcgc ttgtcgcgtg cgcgccttcg
     4021 cgtgttcgcg cacaacgacg ccgtcgatct ggaaaaagtc ctgaagcggg cggcttcctc
     4081 ccgcggccgg cgctggatcg tgaccgaaag cgttttttcc atggacggcg acgtggcgcc
     4141 gttgcgcgag atcgcggcgc tggccgcgcg gtacggcgtg cagacctatg tggacgaggc
     4201 gcacgccacc ggcgtgtggg ggcccgacgg ccgcggcttg gtgaacgaac tcggcctgga
     4261 gaaaaaattg gatgtgtgca tgggcacctt gtccaaggcg ctgggggggg cgggggggtt
     4321 cgtctgcggc cgccgcgatc tggtggaatg gatccacaac cgctgccggt ccttcatcta
     4381 ttccacgggc ctcccgccgg cgtccgcggc cacggccctg aaagccctgg agatttgttt
     4441 gacggaaccg gaccggcgcc gccgcctttt ttcgtcggcg gaccggttgc gccgcgggct
     4501 gtccggtttt ccccgggccc gctcctcctc ggccgccggg atattcgagt cgtcccgcgg
     4561 gcccgtggtc ccttttgtca tcggttccga agcggacgcc ctcgcgctct cccggagttt
     4621 gtggaaaaaa ggtatctttg tcccggcgat ccgtcccccg acggtgccca aggggacggc
     4681 ccggttgcgt ttttccgtca ccgcggaaca cacggaggcg gacgtggacc ttgtgttgga
     4741 agcgatgagg cgccatgcgg gttagcggga gaccgtccct gggtctgtcg gcccggtcgt
     4801 ccggcgtttt tatcatcggg accgataccg gcgttgggaa gaccatggtg ggcgctctgc
     4861 tcgcccggtc tttgcgtgag gcgggggtcc gcgtgggggt gatgaaaccc tacgtcagcg
     4921 gcgggtgggg cgatgtgcgg gctttgaaga gcgcggccgg gctgcaagag ccgttgtccg
     4981 tcctcgcccc ggcgtttttt cggtatccgc tcgccccggc ggcgtcggcg ctgagaggtt
     5041 tcggtccgcg tccggccgcg tggagaggcg ttttgcgggc tttccggcgc ttgcgccggc
     5101 ggtttccgtt cctggtggtg gaggggatcg gcggggtgct ggtgcctttg gaagggcgcc
     5161 tcaccgtggg cgatctggcg cggcgcttga aattgccggt ttggctggtg gcgcggccgg
     5221 ggttggggac gatcaaccat gtgttgcttt ctttggaagc cttgcgccgg cgcggggtgc
     5281 gggtggaacg cgtggtgctg agcggttttt cggggaggac cgcggccgaa aagaccaacc
     5341 cggcgatgtt gcggcgattg acggatcaga aagtttttat actgccgaag gtgacggcaa
     5401 atgtccggcg ggcccttgtg gggcgctggt cggcggaaat caaaaggaaa tggaagggcg
     5461 cggtttgatg gcgaggggga acaaggccga cgcgttggcc gcctgggaca agaaatacgt
     5521 gtggcacccg ttcacccagc aggcggattg ggtgaaggag gagccggtcg tggtggagtc
     5581 ggcgcgcggc tggcatttca aggacacgcg cggccggcgt tatctggacg gcgtttcctc
     5641 gctgtgggtg aacgtgcacg gccacggcga acctgccttg aatgcggcgc tgcgccggca
     5701 actgaagaag atggcgcacg tcacttttct gggattgacg cacgaacccg cggtgcggct
     5761 ggcgagggaa ctggtgaggc tggccccgcg cggactgacg cgggtttttt attcggacaa
     5821 cggctccacc gccgtggaag tggccatgaa aatggctttt caatacgggg cgcagcgcga
     5881 ggggcacgtt cgccgatcgg agtttttcgc gttggccaac tcctaccacg gcgacacgct
     5941 ggggtcggtg tcggtgggag gcatcggcct tttccataaa agattccggc cg
//
LOCUS       DAOTFD010000159         5981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9498_length_5981_cov_4.403139, whole genome shotgun sequence.
ACCESSION   DAOTFD010000159 DAOTFD010000000
VERSION     DAOTFD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9498_length_5981_cov_4.403139"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="P5079_08755"
     CDS             <1..664
                     /locus_tag="P5079_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY30108.1"
                     /translation="FPPPSHILDRLWVLLRTDDTFLRGVLQSHLRLAMGALLAVPLAV
                     FLGLELGLHPVAARLLNPLIAVTYPIPKLAVFPLLMVVFGIGESSKVAIIAIGIFFLV
                     LLSTLQGCRRLFKEDYMDIVTVFRIPPGKRFFSIALRGSLPDILSGLKMGLGYGLVMV
                     VASEFTFSEDGIGFFIWRAWDQFRVVDIYCGLTVLSLSGFLIFAAVDQGTKRLKWYDQ
                     DA"
     gene            complement(652..888)
                     /locus_tag="P5079_08760"
     CDS             complement(652..888)
                     /locus_tag="P5079_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY30109.1"
                     /translation="MEDRLKKIVRSVFNLGPSGSLDGLVYKDHPRWDSLGHIQLILSL
                     EKEFRVKFSSEDVVSVRTLEDIASLLRKKGGQAS"
     gene            complement(900..2252)
                     /locus_tag="P5079_08765"
     CDS             complement(900..2252)
                     /locus_tag="P5079_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRY30110.1"
                     /translation="MNFIGNLGPLIDGKTGEVHEGGALKSRIRRRAAELAVEGILGGD
                     RVALAYGNSPELLIDLLALWERHACAIPLDPGLPASEVERVLQHAEARMLLSPGRTQR
                     LRDSSRREAGEALILYTSGTTALPKGIVHTFEGLASKFRALTENIEAASWKTVLCLLP
                     MHFGHGLICNCLWPWLASRGLVLFPSNDLEVFSRLGEAVDRYRADFFSTVPSIWRWVL
                     HTGAAAPRMKTLKRVHCASAPLDEPLWEQANAWTAGAPVVNVYGTTETAGWIAGTAPG
                     ERPEDGSVGGGWGCEFQVREAREGVGEVWARTDALMKGYLGRPEMTAAVLRAGWYRTG
                     DLGRLDDRGRLHLRGRLDDVINKAGLKIYPDDVEEVLKQCAGVADVCVFGEPHPLFGE
                     EVAAAVVLKGDALSRVQSWCDGKLAGFKIPRKWYPLKEIPRNERGKVSRRRVLELCRG
                     "
     gene            complement(2266..3708)
                     /locus_tag="P5079_08770"
     CDS             complement(2266..3708)
                     /locus_tag="P5079_08770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRY30111.1"
                     /translation="MLFNSVEFFFFLATVLFVYHTGPAVLKRPLLLAASYVFYMSWNP
                     AYGLLLFAVTLLSYAVGRGMESVRRHARILLWAGIGAHLAVMFIFKYYSFFSENVNWT
                     LGRLSLGWDAPAVEILLPIGISFYVFQSLSYMVDVYRRQVGAERNLWLYMLYISFFPQ
                     LVAGPIERASNLLPQLRKDHSFDKEGLRSGLVLIATGLFKKMVLAENLSILVSAAFFR
                     PETRGGLGLYLGALMARYMIYCDIAGYTDIALGCGRAFGIRLSKNFDRPFAARNISEY
                     WQRWHMTLSGWFRDYVFYSLAAGPLARFGVPVLVFVTFLLLGLWHGASWNFVIWGCVN
                     GGMVVVYQSTRGWREKFQVPAALRTAFTFLALVGLPTVFVVTKDVGQSLFAFKKIFGV
                     FSQAGDWWDIGWLFEGRNAVYLLSSAVFIGGMEIAQKLNEKEPVLETIGRWPAGRRRL
                     AYAVLGLVAVAAVGLIFFRQTNTAFVYFQF"
     gene            complement(3708..5252)
                     /locus_tag="P5079_08775"
     CDS             complement(3708..5252)
                     /locus_tag="P5079_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30112.1"
                     /translation="MTRRAARFLAFFGLLLLAAAGGLDNYLQFHNTLARGGRWIGSKL
                     RMAVHPVGSVEFMFTTLPLRRDVLNLGSCWGFQEVLSGAPVRPARVDFDFRCEDAFVV
                     FIFNRGEDRFEGLRLSSERSSDSGYFKATTEGMFLSRVPLAVPRWGGGWNRFSIFSVG
                     GGLEVRINDVSLGTVPARFETGQIGFRGSARPVWVDNIRVWDAEGKVLLRESFGHWKG
                     LRRRAVWAAGLGLLAGFLAWYGSRDWRWGRRISVLALFAAAQWLLSLANFYVPWRPDP
                     PRHEVDVQRARCRAPALFSEHTGYRGEDQLPENFSKNFSGGPAPGVFRILLLGTSQTA
                     GAGAHRVERAFVPRLKALLNERVVPGRRYECLNGSIAGVKAEEILKLYDGEWKSLLKP
                     DMVIFNLSPPGDNPDELMVPMIRFLEKNRGEGIRTILVCEARYVSRTKSRWNRYVKQL
                     GESFGVPVVDMHEHLLKHAADGFLWWDDSHFTPYAHGLVAERLREAVEAFLGDEKNGR
                     KARGRT"
     gene            complement(5249..5521)
                     /locus_tag="P5079_08780"
     CDS             complement(5249..5521)
                     /locus_tag="P5079_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY30113.1"
                     /translation="MKKGRKREWSVYVLRCGDGSFYTGIAKDVAARLEKHRSGKGAAY
                     TRTHLPVELIYQENKRTRSAALIREAGIKAMPRSKKEELVREARKR"
     gene            5727..>5981
                     /locus_tag="P5079_08785"
     CDS             5727..>5981
                     /locus_tag="P5079_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY30114.1"
                     /translation="MKFLVTGAGGMIGANLALHLAAEGHHVHALDDFSTGTRENLRDF
                     TGTVHAGDIRTFDYASLGKLDGIFHQGAITDTTVMDRNLML"
BASE COUNT         1243 a         2044 c         1652 g         1042 t
ORIGIN      
        1 cttcccgccg ccctcgcaca tcctcgatcg gttgtgggtc ctcctccgga ccgacgacac
       61 gttcctgcgg ggcgtcctgc aaagccacct gcggctggcc atgggcgcgt tgttggccgt
      121 ccccctggcc gtcttcctgg gactggaact gggcctgcac cccgtcgccg cgcggctcct
      181 caaccctctg atcgcggtga cttaccccat cccgaaactg gccgtcttcc cccttttgat
      241 ggtcgtcttc gggatcggcg aaagttccaa ggtcgccatc atcgccatcg gcattttttt
      301 cctggtcctg ttgagcacgc tccaaggctg ccggcgcctc ttcaaagaag attacatgga
      361 catcgtgacc gtcttccgga tcccgcccgg caaacggttc ttctccatcg ccctccgagg
      421 ctcccttccg gacatcctca gcgggctcaa gatgggcctg ggctacgggc tggtgatggt
      481 ggtggcctcg gaattcacgt tcagcgagga cgggatcgga tttttcatct ggcgggcgtg
      541 ggaccagttc cgcgtcgtgg acatctattg cggactgacg gtgctgtcgt tatcgggatt
      601 cctgatcttt gcggcggtgg accaggggac gaaacgcctg aagtggtacg atcaggacgc
      661 ctgaccccct tttttccgaa gaagcgaggc gatgtcctcc agcgttcgga cggagaccac
      721 gtcttcgctg gagaacttca cccggaattc cttctccaac gaaaggatga gttggatgtg
      781 ccccagcgaa tcccatcgcg gatggtcctt gtagaccagg ccgtcgaggc ttccggaggg
      841 gcccaggttg aaaacggacc ggacgatttt tttaagacga tcttccatgg actccatgtt
      901 cacccccggc acagctccaa aacccggcgt cggctcacct tgcccctctc gttccgaggg
      961 atctccttca acggatacca tttccgcgga atcttaaaac ccgccaactt cccgtcgcac
     1021 caactctgaa cgcgcgagag cgcgtccccc ttcaacacca cggccgccgc cacctcttcc
     1081 ccgaagagcg ggtggggttc gccgaaaacg cagacatccg ccacgccggc gcattgtttc
     1141 aacacctcct ccacgtcgtc cgggtagatt ttgagccccg ccttgttgat gacgtcgtcc
     1201 aggcgccccc gcagatggag ccgaccccga tcgtccaggc ggccgaggtc tccggtccgg
     1261 taccatcccg cccgaagaac cgccgccgtc atttccggcc ggccgagata ccctttcatg
     1321 agcgcgtcgg tcctggccca gacctccccc acgccctccc gggcctcccg aacctggaat
     1381 tcacatcccc atccgccgcc cacgctcccg tcctccggac gctcccccgg ggcggtgccg
     1441 gcgatccaac cggccgtctc cgtggttcca tagacgttga ccaccggggc cccggccgtc
     1501 caggcgttgg cctgttccca cagcggctcg tccagcgggg ccgaggcgca atggacccgt
     1561 ttcagcgttt tcatcctcgg cgccgcggcg ccggtgtgga gcacccaccg ccaaatcgac
     1621 ggcacggtgc tgaagaaatc cgcgcggtat cggtccaccg cctctcccaa ccgggagaaa
     1681 acctccagat cgttggacgg aaacaggacg agcccccggg acgcgagcca cggccagaga
     1741 cagttacaga tcagcccgtg gccgaaatgc atcggcaaaa gacaaaggac cgttttccag
     1801 gaagccgcct cgatgttctc cgtgagggcg cggaacttcg aggccaaccc ctcaaacgta
     1861 tggacgatcc ctttcggaag agccgtggtc ccggaggtat acaggatcag cgcctctccg
     1921 gcctcgcgac gggaggagtc ccgaagtcgt tgcgtccgtc cgggagacag cagcatccgc
     1981 gcttccgcgt gttgaagcac gcgctccacc tcggaagcgg gcagccccgg atccaacggg
     2041 atggcgcacg catgccgttc ccaaagggcc aggaggtcga tcaggagttc cggagaattc
     2101 ccgtaagcca acgcgacccg gtctccgccg aggatcccct cgacggccag ctccgcggcc
     2161 cggcggcgga tccgggattt cagcgcgccg ccttcatgga cctccccggt cttcccgtcg
     2221 atgagcggcc ccagattccc gatgaaattc atccggtccc ccatgtcaaa attggaaata
     2281 cacgaacgcg gtgttcgtct gccggaaaaa aatcaaaccg acggccgcga cggccaccag
     2341 cccgagaacc gcatacgcga gccggcggcg gccggcgggc cagcgcccga tcgtttccag
     2401 caccggttct ttctcgttta acttttgcgc gatctccatc cccccgataa aaacggccga
     2461 ggagagaaga tagacggcgt tgcgcccctc gaacaaccaa ccgatatccc accaatcccc
     2521 ggcttgggag aaaacgccga atatcttctt gaaggcaaaa agggactgcc ccacgtcttt
     2581 cgtcacaacg aaaaccgtcg gaagcccgac cagcgcaaga aatgtgaagg cggtccgcag
     2641 ggccgccggg acttggaatt tctcccgcca gccccgggtc gactggtaaa cgaccaccat
     2701 ccctccgttc acgcatcccc atatcacgaa attccacgag gcgccgtgcc agagtccgag
     2761 cagaaggaag gtgacaaaca ccaggaccgg cactccgaac cgggccagcg ggccggcggc
     2821 cagcgagtag aacacatagt ccctgaacca accggacagc gtcatgtgcc atcgctgcca
     2881 gtattcgctg atgttcctgg ccgcgaaggg cctgtcgaaa tttttgctca ggcggatccc
     2941 gaacgcccgg ccgcacccca acgcaatatc ggtatacccg gcgatatcgc aatagatcat
     3001 gtaccgcgcc atgagcgcgc cgagatacag cccgagcccg ccgcgggtct ccggccggaa
     3061 aaacgccgcc gacaccagga tcgacaagtt ttccgccaaa accatctttt tgaaaagtcc
     3121 cgtggcgatc aacaccaggc cgctccgcag cccctccttg tcgaaagaat ggtcttttct
     3181 caactgcggc agaaggttcg aggcgcgttc gatgggcccg gcgacgagct gggggaagaa
     3241 cgaaatatag agcatgtaca gccacaagtt gcgttccgcg cccacctgcc gccgataaac
     3301 gtccaccata taactcagag attgaaaaac gtaaaacgag atcccgatgg ggagaaggat
     3361 ctccacggcc ggggcgtccc agcccagact cagccgcccc aacgtccaat tcacgttctc
     3421 cgagaaaaac gaataatatt tgaaaatgaa catcacggcc agatgggcgc cgatcccggc
     3481 ccacagtaaa atccgggcgt gtcggcggac gctctccatc ccgcggccga cggcataaga
     3541 cagcagggtc accgcgaaaa gcaaaagacc gtacgcgggg ttccacgaca tataaaagac
     3601 gtaactcgcc gccagcaaca acggccgctt gaggaccgcg ggtcccgtgt ggtagacgaa
     3661 cagcacggtg gccaggaaga aaaagaattc cacgctgttg aacaacatca ggttctcccc
     3721 cgcgcctttc gcccgttttt ttcatcgccc aaaaacgcct ccaccgcctc ccggaggcgt
     3781 tcggccacca gcccatgcgc gtacggggtg aagtgcgaat cgtcccacca caagaaaccg
     3841 tccgcggcgt gtttcaataa atgctcgtgc atgtccacca ccggcacacc gaacgattcc
     3901 cccaactgtt tgacataccg gttccatcgg gatttggttc gggaaacgta ccgggcctcg
     3961 cacacgagaa tcgtgcggat cccctcgcct cggttctttt caaggaaccg gatcatggga
     4021 accatcagct cgtcgggatt gtcccccgga ggcgacaggt tgaaaatgac catgtcgggt
     4081 ttcaacaagg atttccattc cccgtcgtac agtttcaata tctcttcggc cttcaccccc
     4141 gcgatggacc cattgagaca ttcgtatcgc cggcccggga cgacccgctc attgagaagc
     4201 gccttcaacc gcggcacaaa ggcccgctcc acccggtggg cgccggcgcc cgccgtttgg
     4261 gatgtgccca acaaaaggat ccgaaaaacg cccggggcgg ggccgccgga aaaattttta
     4321 gaaaaattct ccggcaactg atcctccccc cggtagccgg tgtgctccga aaagagcgcc
     4381 ggagcccggc aacgcgcccg ttgaacatcg acctcatgcc ggggaggatc cggccgccac
     4441 gggacataaa agttggccag ggaaagaagc cattgggccg ccgcgaagag ggccaagacc
     4501 gaaatccgcc gcccccaccg ccaatcacgg ctcccatacc acgccaggaa cccggccaac
     4561 aatcccagac cggccgccca gacggcgcgg cggcgaagtc ctttccaatg gccgaacgat
     4621 tcgcgcaaaa gcactttccc ctcggcgtcc cagacccgga tgttgtccac ccacacgggc
     4681 cgggccgatc cccgaaaacc gatttgcccc gtttcgaacc gcgcggggac cgtccccagc
     4741 gaaacgtcgt tgatacggac ctccaaacct ccgccgacgg agaaaatcga aaaccggttc
     4801 caccctccgc cccatcgggg gacggccagc ggaacccggg aaaggaacat cccttccgtg
     4861 gttgctttga aataaccgct gtccgaggag cgttccgaac tgagccgaag gccctcgaaa
     4921 cggtcctccc ccctgttgaa aatgaagacg acaaaggcgt cctcgcatcg aaaatcgaaa
     4981 tccacccgcg cgggacggac cggcgcgccg gagagcactt cctgaaaacc ccagcaactt
     5041 cccagattga ggacatcccg ccggagaggg agcgtggtga acatgaactc cacggaaccg
     5101 accggatgca cggccatccg gagtttggac ccgatccacc gaccgccgcg ggccagggtg
     5161 ttgtgaaatt gaagatagtt gtccaatccg ccggcggcgg ccaacaggag caacccgaaa
     5221 aaagcgagga atctcgccgc ccgccgggtc atcgtttccg cgcctccctc accaactcct
     5281 ccttcttgct cctcggcatg gccttgatcc cggcttctcg gatcagcgcc gcggaccgcg
     5341 tccgcttgtt ctcctggtag atgagctcca ccggcagatg ggtccgggta taggccgccc
     5401 ccttcccgct ccgatgtttt tccagccgcg ccgccacgtc cttggcgatc cccgtgtaga
     5461 aggagccgtc cccgcaacgc aggacgtaga cgctccactc gcgtttccgg ccttttttca
     5521 tgcctcggat catcctcgtg tattttttgt ccatgacggt tcgccgttga acgggtactc
     5581 tcattctatc cattcccgcc gcgtcctgtc caggagaccg cttgtggccg ccggaagatt
     5641 tcataggcgt ggaaaacgcc cggccaaaca gctataattt tcctttgcgg cagctgtccg
     5701 aaaaataacg gtgcgggaga aatcacatga aattcctggt taccggggcc ggcggaatga
     5761 tcggcgccaa cctggccctc catctggccg ccgaaggcca tcacgtccac gccctcgatg
     5821 acttttccac cgggacgcgg gagaacctgc gcgatttcac aggaacggtg cacgccggag
     5881 acatccgcac cttcgattac gcctccctgg gaaaactgga cggcatcttt caccaggggg
     5941 ccatcaccga caccaccgta atggacagga acctgatgct g
//
LOCUS       DAOTFD010000160         5981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9499_length_5981_cov_3.831590, whole genome shotgun sequence.
ACCESSION   DAOTFD010000160 DAOTFD010000000
VERSION     DAOTFD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9499_length_5981_cov_3.831590"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5981)
                     /locus_tag="P5079_08790"
     CDS             complement(<1..>5981)
                     /locus_tag="P5079_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30115.1"
                     /translation="IWPQPYVYYLNLRSTDSASNFETTFSTRVFRIDRNPPVAGLTQP
                     ANASGFSLLTTISGTAGGNERGDMEFSGIDVSSISLQIYDVTDPHYFNGANFNNTTPQ
                     TLFAEVYVGDSSGTWFYQSPNLDSAVNGFQSGHLYRITSWATDKAGNTQTTYVSGVSS
                     NTFYFDNIKPSAGVLIPQQGKPYKSMPTLTGTATDDFSGVAEVQLNVNYLLAGDTWYW
                     TGVDFTSGAYYTAGVSVTNSSAQVTGWTYNPSALNTKLVSRREYRAYALARDVANNWQ
                     AVSATNTFLFDVDWGTATVTLPETDGVIKSTYAPHQATQIQGVAVEMPSHAFAGIERV
                     EIGIRRMPENTYWNGSSWVSSTWVVVNGSETWNYDEPDNPGWENDHLYYVYSRVLDLA
                     GNLSPATTRSFYFDTAQPQSFLIDPNDAFETSLAVLTGTARDTPGTNYTEAGLKDVKV
                     AIQINPGGIGNNWWNGSSGFTIDDGDFSDTGDTGWISASTDVAGNWILTGASTPTWVN
                     NTKYLVKVRSQDNVNNLSLPVTSMTFVFDNSSPTASVTFPVTGSTRSVVSAIQGTAAD
                     VPAVSGKVRIATVTVTVQQAGGDYFNGSIFTSGGPFDLDAVFTGYSSGTWSYDTATLV
                     SKLDSGNEYIVRAKAKDATGLVGPESSPVTFLWDVVRPTTTVTEPSYAGASKYYNTLP
                     TLSGGVSDGPFEGKAAGIADKSQIEVAVYDKTANQWWNGTSGYQPGKNWVQASTYVAG
                     AWQAYFSARVPAPPLTEGNTYLVQSRAKDSAAPANQGPPPNGADPADFVLGVDSVTFI
                     VDKTPPVSKMVRPASGVENVMNAAALVTLSGTVTDALAGFGSTPYEDNVTVSIQEYDP
                     VGLWWNGAFGGASTFTDTSENFYPVSSVNNNAVTVTTWTVAAPNLRHGYKYLIRVKSL
                     DQTLPAPNVETTLSSATVRYDIKAPTATLAVPSLTVLNSLAEITGTVQEEFGVAGVSV
                     TIRSTDAANSWWNGADFAGASAQYANATGLGTWVWDPGDTLRGKLVSGRQYEINVFAR
                     DVAGNVQAVTTKKTFTWDVTKPDSFATHPANNSYFMVGGLTLISGTAKDIIPVNAAVP
                     ELRVYLWEDDGDGEKETNDNPPLVYGDRWWNGSTWSTTETPLVPSVTPGASDPWPWQL
                     NTNLPGPNDMRNGWKHYIYSRAVDNAGNEEDLPANGGTSDRLFYFDNQAPATLTQQPS
                     EGQVINELTMVSGTASDNLNTKRVDIRIKDINDRYWDTSISNWRPLGSPGYPEIWNVA
                     TSSSSSPGAFTWTFTQVPNPWPNGATRIEVRAYDEAGNYAASYSTRNFTFDASRPLSV
                     TTMPWTTDILFSSFSVFAGTATDDLGGVPDNGISNVTLRIKRLPDGDYWYNSGSWLPG
                     ENWVLASGAGSGLDVPFTYTHAKFTDGSAWDTGKTYEVVSKAQDGAGNVQVVFTTHSF
                     IIDKSSPTTDTTNPPNTAAAIDEYYSDVKQLTLITGTAADAPAGVNKVYVSIYEEDTN
                     QFFNGNTFGAATEYWHPATVYPSTWTFSDPDLQDRFYTGRTYRVRAYGVDNIGNIEST
                     ATIAGNRFLHDEVEPLSAVTSPPPGASREPTLNFLQGTAADGLSGVDRVQFAVIIDWD
                     DSGGLAPTAGDYYYDPAVFSGITNSSFSWPVGTDKWIDADNTNWDYGFRDHGAQWVSG
                     KSYLIKSRTIDRSENTEKTGDITYSSFTIAKAAHHFKVIPQNTVFTAGTQQTIRVVAQ
                     AVDNSTAIAYVGQVAFEAVGGPEKAGSDIFQDGLPQDYRFEAGDMGEKDFTIRLRRAG
                     SRVINAWDPDGALEEPLVDTGTVNVSVNPEAADRVQVLMPNQIPAPGTVTGSTGTLAT
                     QTAGQNFVVTVRVTDQFYNLTLSSSPLVTLTMNDPFGSMVPNSFTASSQTIQTIFQTA
                     GAANRTVCATVTPLNSSTSSAITVNPYNPVNLQVLVPGETAVPGSTASGGKGKAGLPD
                     SDGGQSGAQPFVAGTTVTVTVNVTDAYWNLVPTVN"
BASE COUNT          937 a         1780 c         2124 g         1140 t
ORIGIN      
        1 ttgttcaccg tgggcaccag gttccagtac gcgtcggtga cgttcaccgt gacggtcacg
       61 gtggtgcccg ccacgaaggg ctgggcgccg ctctggccgc cgtcgctgtc cggcaaaccg
      121 gccttgcctt taccgccgct ggccgtggaa cccggcaccg cggtctcgcc gggcaccaac
      181 acctgcaggt tcaccgggtt gtacgggttc accgtgatgg cggaggaggt ggaggagttg
      241 agcggcgtga ccgtggcgca caccgtgcgg ttggccgcac cggccgtctg gaagatggtc
      301 tggatggtct ggctggaagc ggtgaagctg ttgggcacca tcgagccgaa cgggtcgttc
      361 atggtgagcg tcaccagcgg actcgacgac agcgtgaggt tgtagaactg gtcggtgacg
      421 cgcaccgtca ccacgaaatt ctgtccggcg gtctgggtgg cgagcgtgcc ggtgctgccg
      481 gtcacggtgc cgggggcggg gatctggttg ggcatcagca cctgcacccg gtcggccgcc
      541 tcgggattga cgctcacgtt gaccgtgccc gtgtcgacca gaggctcctc cagagcgccg
      601 tccgggtccc aggcgttgat cacccgcgag ccggcccggc gcagtcggat ggtgaagtcc
      661 ttctcaccca tatcgccggc ctcgaaacga tagtcctgcg gcaacccgtc ctggaaaatg
      721 tcggaaccgg ctttctccgg cccgcccacc gcttcgaaag ccacctggcc cacgtacgcg
      781 atggcggtgc tgttgtccac ggcctgggcc accacgcgga tggtctgctg ggtgccggcg
      841 gtgaacacgg tgttctgcgg gatcaccttg aagtgatgcg cggccttggc gatggtgaaa
      901 ctggaatagg tgatgtctcc ggtcttttcc gtattttccg aacggtcgat ggtgcggctc
      961 ttgattaaat aggacttacc ggacacccac tgggcgccgt ggtcgcggaa accatagtcc
     1021 cagttggtat tgtccgcgtc gatccatttg tcggtcccca ccggccagga gaaagaggag
     1081 ttggtgatcc cgctgaacac ggccgggtcg taatagtagt cgccggcggt cggcgccagg
     1141 ccgccgctgt cgtcccagtc gatgatgacg gcaaactgca cccggtccac gccggagaga
     1201 ccgtccgcgg cggtgccctg caggaagttg agcgtgggtt cgcgggaggc gcccggcggc
     1261 gggctggtca ccgcggacaa cggctccacc tcgtcgtgca ggaagcggtt gccggcgatg
     1321 gtggcggtgg actcgatgtt gccgatgttg tccacgccgt aggcccgcac acggtaggtg
     1381 cggccggtgt agaacctgtc ctgcaggtcc ggatcggaga aggtccaggt gctgggatac
     1441 accgtggccg gatgccaata ttccgtggcg gcgccgaagg tgtttccgtt gaagaactgg
     1501 ttggtgtcct cctcgtaaat ggacacgtag actttattca cccccgccgg ggcgtcggcc
     1561 gcggtgccgg tgatgagcgt cagctgtttg acgtcggaat agtactcgtc gatggccgcg
     1621 gcggtgttgg gcgggttggt ggtgtccgtg gtgggcgagc ttttgtcgat gatgaaactg
     1681 tgcgtggtga aaaccacctg cacgttgccc gcgccgtcct gcgccttgga gaccacctcg
     1741 taggtcttcc ccgtgtccca cgccgagccg tccgtgaact tggcgtgggt gtaggtgaag
     1801 ggcacgtcca aaccggaccc cgccccggag gcgagcaccc agttctcgcc cggcagccag
     1861 gaaccgctgt tgtaccagta gtcgccgtcg ggcagacgct tgatgcgcag cgtgacgttg
     1921 ctgatgccgt tgtccggcac gccgccgagg tcgtcggtgg cggtgccggc gaacacgctg
     1981 aaggacgaga acaggatgtc cgtggtccac ggcatggtgg tgacggacaa cggccgggag
     2041 gcgtcgaagg tgaagttacg ggtggaataa gaggcggcgt agttgccggc ctcgtcgtag
     2101 gcccgcacct cgatgcgcgt ggcgccgttg ggccacgggt tgggcacctg ggtgaaggtc
     2161 caggtgaacg ccccgggcga agaggagctg ctggtggcca cgttccagat ctcgggatac
     2221 cccggactcc ccagaggccg ccagttggag atggaggtgt cccagtaccg gtcgttgatg
     2281 tccttgatgc ggatgtccac ccgcttggtg ttgaggttgt ccgaggcggt gccgctcacc
     2341 atcgtcagtt cgttgatgac ctgtccctcg gacggctgtt gagtgagcgt ggcgggcgcc
     2401 tggttgtcga agtagaacag ccggtccgac gttcctccgt tggcgggcag gtcctcctcg
     2461 ttgccggcgt tgtccaccgc ccgcgaataa atatagtgtt tccagccgtt ccgcatgtcg
     2521 ttggggccgg gcaggttggt gttcaactgc cacggccacg ggtcgctggc gcccggcgtc
     2581 accgacggca cgaggggcgt ctccgtggtg ctccaagtgc tgccgttcca ccaccggtcg
     2641 ccgtagacca ggggcgggtt gtcgttggtc tccttctcgc cgtcgccgtc gtcctcccac
     2701 aaatacacgc gcagttcggg aacggcggcg ttcacgggga tgatgtcctt ggccgttccg
     2761 gagatgaggg tcaacccgcc caccatgaaa taggaattgt tggccggatg ggtggcgaag
     2821 gagtccggct tggtgacgtc ccaggtgaag gtcttcttgg tggtcaccgc ctggacgttg
     2881 ccggcgacgt cgcgggcgaa cacgttgatc tcgtactgcc gtccgctcac cagtttgccg
     2941 cggagggtgt cgccggggtc ccacacccag gtgcccagtc ccgtggcgtt ggcgtattgc
     3001 gccgaggcgc cggcgaaatc ggcgccgttc caccaggagt tggccgcgtc ggtggagcgg
     3061 atggtcacgc tcaccccggc cacgccgaac tcctcctgca ccgtgccggt gatctcggcc
     3121 agggagttca acacggtcaa ggacgggacg gcgagggtgg cggtgggcgc tttgatgtcg
     3181 taccgcaccg tggcggaaga aagagtggtc tccacgttgg gcgcgggcag cgtctggtcc
     3241 agggacttca cgcgaatcaa gtatttgtag ccgtgccgca ggttcggcgc cgccaccgtc
     3301 caggtggtca ccgtcacggc gttgttgttg acgctggaaa cgggatagaa gttttccgag
     3361 gtgtcggtga acgtggaagc gccgccgaag gcgccgttcc accacagccc cacggggtcg
     3421 tattcctgga tgctcaccgt cacgttgtcc tcgtaggggg tggagccgaa cccggcgagg
     3481 gcgtcggtca cggtgccgga gagggtgacg agcgcggcgg cgttcatcac gttctccacg
     3541 ccggaggccg gccgcaccat cttggagacg ggcggcgtct tgtccacgat gaaggtgacg
     3601 gagtccaccc ccagcacgaa gtccgcggga tcggcgccgt tgggcggcgg gccctggttg
     3661 gcgggcgcgg cggagtcctt ggcgcgcgac tgcaccaaat aggtgttgcc ctcggtgagc
     3721 gggggcgccg gcacccgggc gctgaaatac gcctgccacg cgccggccac gtaggtgctg
     3781 gcctgcaccc agttcttgcc cggctgatag ccggaggtgc cgttccacca ctggttcgcg
     3841 gtcttgtcgt acaccgccac ctcgatctgg gacttatcgg cgatgcccgc cgccttgccc
     3901 tcgaacggac cgtcggacac gccgccggag agggtgggca gggtgttgta atacttggac
     3961 gcgcccgcgt aggagggttc cgtcaccgtg gtggtgggcc gcaccacgtc ccacaggaag
     4021 gtcaccggcg aactctccgg accgaccagc cccgtggcgt ccttggcctt ggcccgcacg
     4081 atgtattcgt tgccgctgtc gagcttgctg acgagcgtgg cggtgtcgta actccaggtg
     4141 ccggaggaat aacccgtgaa caccgcgtcc aggtcgaacg gcccgccgga ggtgaagatg
     4201 ctcccgttga aatagtcccc gcccgcctgc tgcaccgtga cggtgacggt ggcgatacgc
     4261 accttaccgc tcaccgccgg cacgtccgcc gcggtgccct ggatggccga caccaccgaa
     4321 cgcgtggacc cggtgaccgg gaacgtcacg gaggcggtgg gcgagctgtt gtcgaacacg
     4381 aaggtcatgg aggtcaccgg cagggacagg ttgttgacgt tgtcctgcga gcgcaccttc
     4441 accaaatatt tggtgttgtt gacccaggtg ggggtggagg cgccggtgag gatccagttg
     4501 cccgccacgt cggtggaggc gctgatccag ccggtgtccc cggtgtcgct gaaatcgccg
     4561 tcatcgatgg tgaacccgct tgaaccgttc caccagttgt tgccgatgcc gccggggttg
     4621 atctggatgg ccaccttcac gtccttcagc ccggcctcgg tgtagttggt gccgggcgtg
     4681 tcgcgggccg ttccggtgag aaccgccagg ctcgtctcga acgcatcgtt gggatcgatg
     4741 aggaacgact gcggctgggc ggtgtcgaaa tagaaactgc gcgtggtggc cggcgagagg
     4801 ttcccggcca ggtccagcac ccgggaatag acgtaataga ggtggtcgtt ttcccagccg
     4861 gggttgtccg gttcgtcgta gttccaggtc tccgatccgt tcaccaccac ccaggtggaa
     4921 ctcacccaag agctgccgtt ccagtacgtg ttctccggca tccggcggat gccgatctcc
     4981 acccgttcga tgccggcgaa agcgtgggac ggcatctcca ccgccacgcc ctggatctgc
     5041 gtggcctgat gcggcgcgta ggtggacttg atgacgccgt cggtctcggg cagggtgacg
     5101 gtggcggtgc cccagtccac gtcgaaaagg aaggtgttgg tggccgaaac agcctgccag
     5161 ttgttggcca cgtcgcgcgc cagggcgtag gcgcggtatt cccgccgcga gaccagtttg
     5221 gtgttgagcg cggaggggtt gtacgtccat ccggtcacct gagcactgga gttggtgacc
     5281 gaaacgccgg cggtgtagta cgcgcccgag gtgaaatcca cgccggtcca gtaccaggtg
     5341 tccccggcca gaaggtagtt gacgttcagc tggacctcgg ccacgccgga gaagtcgtcg
     5401 gtggcagtgc cggtgagggt gggcatggac ttatacggtt tcccctgctg aggaatcagc
     5461 accccggcgg acggctttat gttgtcgaaa tagaaggtgt tggaggacac gccggagacg
     5521 taggtggtct gggtgttgcc cgccttgtcc gtggcccagg aggtgatgcg gtagaggtgc
     5581 ccgctctgga acccattgac agcgctgtcc aggttggggc tctggtagaa ccacgtgccg
     5641 gaggaatctc ccacgtagac ctcggcgaac aacgtctgcg gggtggtgtt gttgaaattc
     5701 gcgccgttga aataatgcgg atcggtgacg tcgtagatct gcagggagat gctgctgacg
     5761 tcgatgccgg aaaactccat atctcctcgc tcgttgcctc cggcggtgcc cgagatggtg
     5821 gtcaacaggc tgaacccgct ggcgttggcc ggctgggtga ggccggcgac cggcgggttc
     5881 cggtcgatgc ggaaaacacg cgtggagaac gtggtctcga agttgctggc cgagtccgtg
     5941 gaacgcaggt tcaggtaata cacgtagggc tggggccaga t
//
LOCUS       DAOTFD010000161         5965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9528_length_5965_cov_4.345516, whole genome shotgun sequence.
ACCESSION   DAOTFD010000161 DAOTFD010000000
VERSION     DAOTFD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9528_length_5965_cov_4.345516"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="P5079_08795"
     CDS             <1..628
                     /locus_tag="P5079_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY30116.1"
                     /translation="VMLDGESVRKRMLDAVRSGDPRLRGYACTALGILRGPDAMEALK
                     IALDDEDLAVRKSAVEGLARSADRADVLDALKLLLGERDPQLRLAVLAALLRINAEEA
                     LPSLELSLADPDPWVRFKTIKLTAQVRNGKTLDVLMRNYSNQDDAGRRAICDALADFP
                     GKDKVVVPFLEEKKGQAGGDERLTQSISKALQKIRDRKSGGRGGKKAS"
     gene            871..1698
                     /locus_tag="P5079_08800"
     CDS             871..1698
                     /locus_tag="P5079_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRY30117.1"
                     /translation="MLLTTEEFTELRDMIYAESGIYFKEEKTYFIVTRLTARMGALNC
                     ADVSEYIQYLKFDKSKAELYRLIDALTTNETFFYRDLNQIEGFATEVLPQIVKRKREM
                     GQRTIRLWSAGCSTGEEPYTLAIILHEYLKDLICWNIQLFATDISQRVLNACRIGTYP
                     PRSMKDVPEDIRERYFEKVDHTYKINFDIRKMVQFSMLNLSSRGLVRTMSNMDCIFCR
                     NVLIYFNDESANQVVNSFYDNLTPGGFLFLGHAESIHRYSAAFRMVKLRSSFVYKKE"
     gene            1710..2084
                     /locus_tag="P5079_08805"
     CDS             1710..2084
                     /locus_tag="P5079_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30118.1"
                     /translation="MTAKKILVIDDSPTMRSLHSYILKSGGFDVETAENGVMGIEKLY
                     SDPAIVLCVVDINMPKMDGIQFIRTIRGEEQYRDLPVIVVTSEEEEKDRRRGLEAGAN
                     IYMVKPTQPEKLVMHVKMLLGV"
     gene            2138..4222
                     /locus_tag="P5079_08810"
     CDS             2138..4222
                     /locus_tag="P5079_08810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRY30119.1"
                     /translation="MVKEFVNEFREHLENVDRSLLSLEKDPGNQSLVNEIFRAAHSIK
                     GGASYLNLARVIEFTHKLENILDNLRKGMLLVTPAVVDVLFHATDVLKQLINCFVQNG
                     NDEGVDIQKIMGELATIEGPGGVDTKKKKEKPAPEASSGGGSEESTVLDVFYSTGMQH
                     LDVLEAVLREAAAGKSGREQYDRLFRAFCGIKTASVVMQEQGLKDLADRAAQAADQLR
                     QGLLKESPAVLEFFQKILEVVHRHLERKRKDPSIRFDAASLLSEAQYFAPASGTVAGR
                     TEGGAEGAVRVQGEQTIRVGEGKLDHMMNLIAELIIVRNRFNILSKSDGGRRDVSLED
                     LGDATATFSRISNELQLLIMDIRMIPISTVFNKFPRMVRDFAKAASKKIELVIEGEQT
                     ALDKSVIEKIGDPLVHLVRNAIDHGIEPPGPRAKSGKSETGKVRLAARNEGENVVVEV
                     EDDGRGMDPEALKRSAIAKGLISEEKARSMSPEEAIDLIFEPGFSTAETVTDISGRGV
                     GMDVVKSNIKRLNGEITIKTEVKKGSLISIRLPMSLAIVKVLMTGVGNALYSFPLSSV
                     GMTVRVSAQDVFSLGAKGKAIHLHGKTVMVEPLSDLLGLPAGPSVNGKISIVLLAVAD
                     KQFGVLVDRFYEQQEIVVKPLEGLLNTPCISGSAVLGNGRICLVVDVVELMKLALLRH
                     EHFSKERGDPHV"
     gene            4215..4571
                     /locus_tag="P5079_08815"
     CDS             4215..4571
                     /locus_tag="P5079_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30120.1"
                     /translation="MSKRVLIADDSGAMLKIGAMVLGKLGLTVLTATDGNEAVQKAVA
                     EKPDIILLDAEMPELDGWGACMEIRKTMPTVPILMCTGHDLTGEEGQLKEAGANGFIS
                     KPYNPASMEEKLKPYL"
     gene            4952..5587
                     /locus_tag="P5079_08820"
     CDS             4952..5587
                     /locus_tag="P5079_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30121.1"
                     /translation="MEKKETFTTYEISQFCNVFVTTVANWIDEGKLSAYRTPGGHRRV
                     LRQDLLAFLEKYKMPIPEILLEVREKKLLIVDDNPQTLDMLQQALLKQNPGYLIRMVG
                     NGFDAGKEVAVFRPHLIILDIVLPGLDGLQVFQNLKKDPLTRNIHVIAITGHDEPSIR
                     EEFVKLGVDDYMEKPLNLERLTQRVKEILEKAAPLGPGNVAKPSPSSNEAV"
     gene            5646..>5965
                     /locus_tag="P5079_08825"
     CDS             5646..>5965
                     /locus_tag="P5079_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30122.1"
                     /translation="MNARQKITTSLLTHRRKRVVAWALFAAAFLAVGLRAETVPPEEL
                     FSHYLYFSSFSDALVEHARLLAERMIARRDLGPASRVIEAASNDGYLLQHYLRKGIPV
                     LGIEP"
BASE COUNT         1304 a         1553 c         1962 g         1146 t
ORIGIN      
        1 ggtgatgctg gatggggagt ccgtccggaa gcggatgctc gacgccgtgc ggtccgggga
       61 cccgcgcctt cgcggctacg cctgcacggc gttgggtatt ttaaggggcc cggacgccat
      121 ggaggcgctt aagatcgccc tcgacgacga ggacctggcg gtgcggaaat cggcggtgga
      181 ggggctggcc cgctcggccg accgggcaga cgtgttggac gcgttgaagc tgttgttggg
      241 ggagagggat cctcaattgc ggttggcggt gttggcggcc ctgttgagga tcaacgcgga
      301 ggaggctctt ccttccctgg aactctcctt ggccgatccg gacccctggg tgcgtttcaa
      361 aaccatcaag ctgacggccc aggtgcgcaa cggaaagacc ctcgatgtgc tgatgaggaa
      421 ttattcgaac caggacgacg ccggccgccg cgccatttgt gatgcgttgg cggatttccc
      481 ggggaaagac aaggtcgttg tcccgttcct tgaggagaaa aaagggcagg ccggggggga
      541 cgagaggttg acgcagagca tttcgaaggc cctgcaaaag atccgggatc ggaaaagcgg
      601 cggccgtggg ggaaagaaag cgtcttgagg ggggaagggt ccctgggcgg cttccccttc
      661 gctgccgacg gttttgaagg gacgtggtcc cttcggcggg acatttcgtt ttgggttcga
      721 tgacatgaaa aaatgcttga tcggcgccat tccggaagtg ctggccggtg ttggtcgtgc
      781 cggcgtcgtg ggggccgttg ctccacggcg cggggattct tctgcgcgcg gcgtggtctt
      841 tgagagggcc tcggcgggga cggggcgacg atgctcctga cgaccgaaga attcaccgag
      901 ctgcgggaca tgatctatgc cgagtcgggc atctatttca aggaggagaa gacctatttc
      961 atcgtgaccc ggttgacggc ccggatgggg gcgctgaatt gcgcggatgt gagcgaatac
     1021 atccagtact tgaaattcga caagtccaag gcggagctct atcggctgat cgacgccttg
     1081 accacgaacg agacgttctt ctatcgggat ttgaaccaga tcgaaggttt tgcgacggag
     1141 gtcctgccgc agatcgtgaa acggaagagg gagatggggc agcggacgat ccgcctgtgg
     1201 agcgcgggct gttcgaccgg agaggaaccg tacactctcg ccattatcct ccatgaatat
     1261 ctcaaggacc tgatctgctg gaacatccag ctgttcgcca ccgacatcag ccagcgggtg
     1321 ctgaacgcct gccggatcgg cacctacccg ccgcgttcca tgaaggacgt gccggaggac
     1381 atccgggaga gatatttcga gaaggtggac cacacctaca agatcaattt cgacatccgg
     1441 aaaatggtcc agttcagcat gttgaacctc tccagccggg gcctggtgcg gacgatgagc
     1501 aacatggact gcattttctg ccggaacgtg ctgatctatt tcaacgacga atccgccaac
     1561 caggtcgtca acagttttta cgacaacctg actcccggcg ggtttctgtt cctggggcat
     1621 gcggaatcga tccatcgcta cagcgccgcg ttcaggatgg tcaagttgag gtcgtcgttc
     1681 gtttataaaa aagaatgagg ggatgaaata tgacggctaa aaaaatcctg gtgatcgatg
     1741 attcgccgac gatgcgttct ctgcacagct acatcctcaa gtccggaggg ttcgatgtgg
     1801 agacggcgga gaacggcgtg atggggatcg aaaagctcta ttccgacccc gccatcgtcc
     1861 tttgcgtggt ggacatcaac atgccgaaaa tggacggcat ccagttcatc cgcacgatcc
     1921 gcggggagga gcaataccgg gaccttcccg tgatcgtggt gaccagcgaa gaggaggaaa
     1981 aggaccggcg gcggggcttg gaggcgggag ccaacatcta catggtcaag cccacccagc
     2041 cggagaagct ggtgatgcac gtcaaaatgc ttctgggcgt ctgacgcccg tccctgggag
     2101 gtgtgccatt agcaacgaaa tcggcaatga tccggagctg gtcaaggagt tcgtcaacga
     2161 attccgggaa catctcgaga acgtcgaccg aagcttgttg tcccttgaaa aggaccccgg
     2221 gaaccagagc ctcgtcaacg agatcttccg ggccgcccac agcatcaagg ggggggcttc
     2281 gtatctcaac ctggcccggg tcatcgagtt cacgcataag ctggaaaaca tcctggacaa
     2341 cctccgcaag gggatgctcc tcgtgacccc cgcggtggtg gacgtccttt tccatgcgac
     2401 cgacgtcctg aaacagctga tcaattgttt cgtccagaac ggtaacgacg agggcgtcga
     2461 catccaaaag atcatgggcg agctggccac catcgaggga ccgggcggcg tcgatacgaa
     2521 gaagaaaaaa gagaagccgg cgccggaggc gtcctccggg gggggctcgg aagaatcgac
     2581 ggtgctggat gtgttttatt cgacgggcat gcagcatttg gacgtgctgg aggcggtcct
     2641 gcgggaggcg gccgccggaa agagcggccg cgaacagtac gacaggctgt tccgcgcttt
     2701 ttgcggaata aaaacggcgt ccgtggtgat gcaggagcag gggctgaagg acctggccga
     2761 ccgggcggcg caggccgccg atcaactccg gcagggcctg ctgaaggaga gcccggcggt
     2821 tctggaattt ttccaaaaga tactggaggt ggtccatcgg catctggagc ggaagaggaa
     2881 ggacccgtcg atccggttcg acgccgcttc gctgttgagc gaggctcaat atttcgcgcc
     2941 cgcgtccggg accgttgcgg ggaggacgga ggggggggcg gagggggccg tccgcgtcca
     3001 gggcgagcag accatccgcg tcggcgaagg gaaactggac cacatgatga atttgatcgc
     3061 cgaattgatc atcgtgcgga accggttcaa catcctgagc aagtccgacg gcggccggcg
     3121 ggacgtttcc ctggaagacc tcggcgacgc gaccgcgacc ttctcccgta tttccaacga
     3181 actccagctg ttgatcatgg atatccggat gatcccgatc tccaccgttt tcaacaagtt
     3241 tccgaggatg gtgcgggatt ttgccaaggc ggccagcaaa aagatcgagt tggtgatcga
     3301 gggggaacag accgcgctgg acaagagcgt catcgagaag atcggggacc cgctcgtgca
     3361 cctggtccgg aacgccatcg accatggaat cgagccgccg ggaccgcgcg cgaagtcggg
     3421 gaaaagcgag accgggaagg tgcggctcgc ggcgcggaac gaaggggaaa acgtcgtcgt
     3481 ggaggtggag gacgacggcc gcggcatgga tcccgaggcc ctcaaacgga gcgcgatcgc
     3541 caaggggctg atttcggagg aaaaagcgcg gtccatgagc ccggaagagg ccatcgattt
     3601 gatcttcgag ccggggttca gcacggcgga gaccgtgacc gatatctcgg ggcggggcgt
     3661 cggcatggac gtggtcaaaa gcaacatcaa acgcctgaac ggggagatca cgatcaaaac
     3721 ggaggtcaaa aaggggagcc tcatctcgat ccggctgccc atgtcgctgg cgatcgtgaa
     3781 ggtgctgatg acgggcgtgg ggaacgcgct gtattccttt cccctctcct ccgtcggaat
     3841 gaccgtccgc gtttcggcgc aggacgtgtt cagcctgggg gcgaagggga aggccatcca
     3901 tctgcacggg aaaacggtca tggtggaacc gttgagcgat ctgctggggt tgccggcggg
     3961 gccgtccgtg aacggaaaga tatccatcgt cctcttggcc gtggccgaca agcagttcgg
     4021 cgtgctggtg gaccggtttt atgagcagca agagatcgtc gtgaaaccgc tggaggggct
     4081 gttgaacact ccctgcatca gcggttcggc ggtgttgggg aacgggcgga tttgtcttgt
     4141 ggtggacgtg gtggagctga tgaaattggc tctgttgcgg cacgagcatt tttcgaaaga
     4201 aagaggtgat cctcatgtct aagcgtgttc tgatcgcgga cgattccggc gccatgctga
     4261 agatcggcgc gatggtgctg ggcaagctgg gattgacggt gctgacggcg acggacggca
     4321 acgaggcggt tcagaaagcc gtcgcggaaa aaccggacat catcctgctg gatgccgaga
     4381 tgcccgagct tgacggctgg ggcgcctgca tggagatacg gaaaaccatg ccgacggtgc
     4441 cgatcctcat gtgtaccgga catgacttga ccggggagga ggggcagctg aaggaagccg
     4501 gggccaacgg gttcatttcg aaaccctaca acccggccag catggaggaa aaacttaaac
     4561 cgtatctctg accggaggcg cgaggaagaa gcgtcgagag aatccagggc cgtccggatt
     4621 tttaaagtaa attgatttcc agcggattga cgcggagacg gcctggccgg ttcgaggggg
     4681 gaacgcattc cgtctgtttt ttcaatcggc cgctccgggc catcggtttt tcggggtaaa
     4741 agactaaaaa acgaataatc cgaacaattt tccgaagggg cgttcaaatg aaaaatcatc
     4801 ccatcgggtt cctgccatcc cccaaaaatc ataagaaatc ccttgaaata aatcagttca
     4861 gctttatact taatagtagc aaaaccacaa ttatcaacaa aaacgattaa ttgacatctg
     4921 tcttgatatt tatagtttct aggaggggaa aatggaaaaa aaggaaacgt ttacgaccta
     4981 tgaaatcagt caattttgta acgtttttgt tacaaccgtt gccaattgga tcgatgaggg
     5041 gaaactttcc gcctatcgca ctcccggggg acatcgccgg gtgttgaggc aggatcttct
     5101 ggcgtttttg gagaaataca agatgccgat cccggagatt ctgttggagg tccgggaaaa
     5161 gaaactcctc atcgtggacg acaaccctca gaccctggat atgctccaac aggcgctgct
     5221 caagcagaac ccgggctatc tcatccgaat ggtgggaaat gggtttgacg cgggcaaaga
     5281 ggtggccgtt tttcggccgc atctgatcat cctggacatc gttcttccgg gtctggacgg
     5341 ccttcaggtc tttcagaacc tgaagaaaga cccgctcacg cggaacatcc acgtcatcgc
     5401 catcaccgga cacgacgaac cttccattcg ggaggagttc gtcaagttgg gggtggacga
     5461 ctatatggag aagccgctga acctggaacg tttgacgcag cgggtgaaag agattctgga
     5521 gaaggccgcg ccgctggggc cggggaatgt cgcgaagccg tcgccttcct cgaatgaggc
     5581 ggtgtagctg atttttggcg aggtcggagc acgccggagg ggaagagccg cctaacgcat
     5641 ttccgatgaa cgcgaggcaa aaaatcacca catctctgtt gacgcatcgc cggaaaaggg
     5701 tcgttgcctg ggcccttttc gcggcggcgt ttttggccgt ggggctccgg gccgaaaccg
     5761 ttccgcccga ggagttgttc agccactacc tttacttctc ctccttctcc gacgcgctgg
     5821 tggaacacgc ccgcctcctg gcggaacgga tgatcgcgcg gcgggacctc ggaccggcca
     5881 gccgggtgat cgaggcggcc agcaacgacg gctacctgct ccagcactat cttcggaagg
     5941 ggattccggt cctggggatc gagcc
//
LOCUS       DAOTFD010000162         5965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9529_length_5965_cov_3.842978, whole genome shotgun sequence.
ACCESSION   DAOTFD010000162 DAOTFD010000000
VERSION     DAOTFD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9529_length_5965_cov_3.842978"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5965
                     /locus_tag="P5079_08830"
     CDS             <1..>5965
                     /locus_tag="P5079_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY30123.1"
                     /translation="EKELAAVLKKTRALVAYNQVPAKIYAHIREKGPLASAQDLIAAR
                     LGIGKKAMEKLAPLVGFGPVAVAPAAETVPRLDAAALASLAHEVVQRLDDATRRALVE
                     EDAIETETDRGILIEKYLMSLVGNIRGPTAPLDDEDWALLYDAIVQESAVVVARTPAP
                     APEAPAALQLELFQSEEVSSTPAAPAAEQAAPKKLSKTVWISLAIATALGAFAYFYGL
                     ETVYQALLQLIPQGQAGAGLVGLAKLQASFFGFVSYTTYFLGNVIVPLTALAVLGRIF
                     VSGSLSQTWLGRKVAQPISSFLKNNRVSKGLGGLLQKGAAKSRVIAVLLHLPKEVGQT
                     LYGFAFEKGDRLFQIFLSLQTGVEPSLRGELSWSERAFVKADKGMGKIRLPKVVKGVA
                     LLLLLPALAAVGILGKRVMLKFYSTIGQTLIVGVLSSLFAVMGPTKGALLLVLLPGLF
                     AVGMVVRSVAAKQRPSWQKTVLIAFLLAVTIGGVPLILSANLGSSLSVLSTILNYNLL
                     DPLNTGWTVPYAHHGVLGAVIQLFSPFGIASSYMLAVIMTFVSGARKIRKAAKAANKP
                     VPGWVRAFWRSFRQPVHVILGRDEKPKEVSLWRASLETAFSPSKYGLSSLHAVGLEIE
                     IFKWLAGFLGPLNDVVLKIEDSSRVSVIGAGDQMLSTITRPLGFEVGDVVSYAPGTGG
                     LNLQDIQQIQGLKWSADRQARELDTLTRGLQGLREGTADAATRDQWQKLVKQAEQLQS
                     AQVERLAELQKQGVVKADTLNMLRASLERQRAPLRDATAALQRGGRLSETQAQTLGEV
                     LEMQKGQVEALQKQANELIARGEAGAPSFAQRISRSFRGVVDLFRPSPKETKEKPAVT
                     PPTGVAAEGQEPPTPFTAPPSVGEQPKERREPAATPSQERAVEPEPLQVSPPARRPAP
                     KTPADFMPHNVVATQRQMVTEIALAGEASADTMVARADAAAEAELGLVERRDSMGDGR
                     YEASRVNSDGTPRVHHGIDYAAEEGTPVMALAGGTAQFSYQPNGAGYRVSIYAADGTV
                     LKYFHLTENLPLSEQEMAQLKKGEFVQKTVKFGEVVGAAGRTGNADNPNIASHLHFEF
                     WRNGEPRNPFTDFLQPNGLNPFTGRVGQEGPAVAGQTPVVPRPEVSAEQKKAEAAAPE
                     RAIPKKYDPASLAADGRVTLAEALSGVSDVAPVRIREQEVKRQRSFFDLIRNRFTMDI
                     FASYRLSPGKLFREYEDPTRAAETTETPVANPVNNLLRQVSPSVSAESLVGLRRWEDR
                     QGRKETGRAPVRGAQAKPAASSAASLGMRVETAAAETARPAAAPRAVAPTTAQQVDRL
                     VQGLGTPSSVSRPTLPGLGQSPSPVFGPAARPASVGPSLLDMGDLHQPAAFLPAAAAS
                     PAFYPAHWQLDAVVQGWLSGEAVHCFIGEDGRVRLSIFQDANGNGVQDSNEKPLTETD
                     PLLLDHLVNAIGKMDGARIYLDGQTLVFADKLGREFTRLDLVKLAGQTERGKPTAEAT
                     KAMAILGFYAGLALQSTVNADGTVTLRILDENGKPVTTPQTVDLDTFLKTQVAISQLG
                     GTSEFLNDRTIVYRDANGKEYSRVDLVVLAGNSSKGAEAAAILGWYAGLSLNSTVTPE
                     GKVMMKIGGQTPVEVDLDTFLRTQTAISELAGSKEFLNDGTIVYRDRTGKGYSRVDLL
                     RLAGTSSKGAEAAAILGWYAGLALNSTVTPEGKVMMSIGGQTPVEVDLDTFLRTQAKI
                     SELAGAQAFLDDKSIVFKSGGKSYSRVDLLIASGKATPEGLKAAAVLGWYGGLALQST
                     MKDGKVFLQITDADGNRLPIPESVDLDTFLRMQVAISELGGAKEFLDDRTIVFKDRNG
                     QEFTRVDLVQMAGTPEGVQAAAILGWYAGLALNATITEDGKVRLNIGGAVPAEVDLDT
                     YLMTQAKISELAGAKAFINGTAIVFVDAAGREYSRLDLLTEAGKLTPEGTRAAAVLGW
                     YAGLALRCTVSGGRVTLHITDENGNALPTP"
BASE COUNT         1097 a         1846 c         1994 g         1028 t
ORIGIN      
        1 gaaaaagaac tggccgccgt tctgaaaaag acccgcgcgt tggtcgctta caaccaggtc
       61 cccgcgaaga tctacgccca tatccgtgaa aagggaccgc tggcttccgc ccaagacctg
      121 atcgccgccc ggctcgggat cggcaaaaag gccatggaaa aactggcgcc gctcgtcggt
      181 ttcgggccgg tcgccgtggc gccggcggcc gaaaccgtcc cgcggctgga cgcggccgcc
      241 ctggcgtcgc tggcccacga ggtggtgcag cgcctggacg acgcgacccg tcgcgccctc
      301 gtggaggagg acgcgatcga gacggagacg gaccgcggga tcctcatcga aaaatacctg
      361 atgagccttg tcggtaatat tcgcggtccc accgccccgc tggacgacga ggattgggcg
      421 ctcctgtacg acgccatcgt gcaggaatcg gccgtcgtcg tggcccggac gccggcgccc
      481 gcgccagaag cgcccgccgc cttgcagttg gaactgttcc aatcggagga ggtttcctcg
      541 acccccgccg cgccggccgc ggaacaagcg gcccccaaaa aactgtccaa gaccgtctgg
      601 atttcccttg ccatcgccac cgccctcggc gccttcgcct acttctacgg tttggagacg
      661 gtctatcagg cgctcctgca actcatcccc caaggacagg ccggcgccgg tttggtgggg
      721 ctggccaaac tgcaggcctc cttcttcggg ttcgtttcct acaccaccta tttcctgggg
      781 aatgtcattg tccccctgac cgctctggcg gtcttggggc gcatcttcgt cagcggttcc
      841 ctctcccaaa cctggctcgg ccgcaaggtg gcccagccga tctcttcttt cctgaaaaac
      901 aaccgcgttt cgaaggggct gggcgggctt ctgcaaaaag gcgccgccaa aagccgcgtt
      961 atcgccgttt tgctgcacct cccgaaagag gtcggtcaaa cactgtatgg tttcgctttt
     1021 gaaaaaggcg accggctctt ccaaatcttc ctttcgttgc aaaccggcgt cgaaccgtcg
     1081 ctgagagggg aactcagctg gagcgaacgg gctttcgtaa aagcggacaa gggcatgggc
     1141 aagatccgcc tgccgaaagt ggtgaaaggc gtggcgctcc ttctgctgtt gccggcgctg
     1201 gcggcggtgg gcattctggg caaacgggtc atgctgaagt tctattccac catcgggcag
     1261 acgctgatcg tcggagtctt gtcctccctg tttgcggtga tggggcccac caagggggct
     1321 ctcctcttgg ttctcctccc cgggttgttc gccgtcggaa tggtggtccg ttcggtcgcg
     1381 gcgaaacagc ggccttcttg gcagaagact gttctcatcg cgttcctgtt ggccgtgacc
     1441 atcggggggg tcccgctgat tttgagcgcc aacctcggct cctcgctttc cgtcctcagc
     1501 accatcctta actacaacct gctggatccg ctcaacaccg gctggaccgt cccctacgcg
     1561 catcacgggg tgttgggcgc cgtgatccaa ctgttctctc cgttcgggat cgccagttcc
     1621 tatatgttgg cggtcatcat gacgtttgtg tccggcgccc ggaaaatccg gaaagcggcg
     1681 aaggccgcga acaagccggt gccgggctgg gtgcgggcgt tctggcgttc gttccggcag
     1741 ccggtccacg tgatcctggg gagggatgaa aagccgaagg aagtctctct ctggagggct
     1801 tccctggaaa cggcgttcag cccgtcgaaa tacgggttgt cgagcttgca cgccgtcggc
     1861 ctggaaatcg agatattcaa atggttggcg ggatttctcg gtccgctgaa cgacgtggtg
     1921 ctgaagatcg aagactcttc ccgcgtgagc gtcatcggcg cgggcgacca gatgttgagc
     1981 accatcaccc ggccgctcgg ctttgaagtc ggcgatgtcg tcagttatgc ccccggcacc
     2041 ggcgggctca atctgcagga catccaacag atccaggggc tcaaatggtc ggccgaccgc
     2101 caggcgcgcg aattggatac gttgacccgg ggattgcaag gactccgaga gggaacggcc
     2161 gatgccgcca cccgtgatca gtggcagaag ctggtgaaac aggcggaaca gctccaatcg
     2221 gcccaggtgg aacggctggc ggagttgcag aagcaagggg tggtcaaagc ggacaccttg
     2281 aacatgttgc gcgcctcgtt ggaaaggcag cgggctcctt tgcgggacgc cacggcggcc
     2341 cttcagaggg gcggacggct ttccgagacc caggcgcaaa cgctgggcga ggtgttggag
     2401 atgcagaaag gccaagtgga ggcgctgcaa aagcaggcga acgaattgat cgcccgtggg
     2461 gaggccggcg ccccgagctt tgcccagagg atcagccgca gtttccgggg ggtggtggac
     2521 cttttccggc cgtcaccgaa agaaaccaaa gagaagcccg ccgtgacccc gccgacgggg
     2581 gtcgccgcag agggccaaga acccccaacg cctttcaccg cgccaccgtc ggtcggggaa
     2641 cagccgaagg agcggaggga accggccgcc acgccttccc aagagagagc ggtggaaccg
     2701 gagccgttac aggtgagccc gccggcccgc cggcccgccc cgaagacgcc ggcggatttc
     2761 atgccgcaca acgtggtcgc cacgcagaga caaatggtca cggagatcgc cttggccggg
     2821 gaggcgtccg cggacacgat ggtggcccgc gccgacgccg ccgccgaagc cgaactgggg
     2881 ctggtggaac gccgcgacag catgggcgac ggccgctatg aggcctcgcg ggtgaattca
     2941 gacgggacgc cccgggtcca tcacgggatc gattatgccg ccgaggaggg gaccccggtg
     3001 atggcgttgg ccggggggac ggcccagttc tcgtatcaac cgaacggcgc cggatatcgc
     3061 gtttccattt acgccgccga cggcacggtc cttaaatatt tccacctcac ggagaacctg
     3121 cctttgagcg agcaggaaat ggcccagttg aagaaaggcg agttcgtgca gaagaccgtg
     3181 aagttcggcg aggtcgtggg cgccgccggc cgcaccggca acgccgacaa ccccaacatc
     3241 gcgtcccatc tccatttcga attttggcgg aacggcgaac cccggaatcc cttcacggat
     3301 ttccttcagc cgaacggact gaacccattc acgggccgcg tgggccagga aggaccggcc
     3361 gtcgccggac agacccccgt cgttccgcgg ccggaagtct cggcggaaca gaaaaaagcg
     3421 gaggccgccg cgccggaacg ggcgatcccc aagaaatacg atccggcttc cctggcggcc
     3481 gacggtcgtg tgacgctggc ggaggctctc agcggtgttt cggacgtggc ccccgtgagg
     3541 atccgcgaac aggaagtgaa gaggcaaagg agtttcttcg acctgatacg gaaccggttc
     3601 accatggaca tctttgcatc ttaccgcctt tcgccgggga aactgttccg ggagtatgag
     3661 gatccgaccc gggccgccga aacgacggaa actcctgtcg cgaacccggt caacaatctt
     3721 ttgaggcagg tcagcccgtc cgtgtcggcg gagtcgctgg tcggtcttcg ccgctgggaa
     3781 gaccgtcagg ggcgtaagga aacgggccgc gcgccggtcc gcggcgctca ggcaaaaccg
     3841 gccgcttctt cggcggcatc cctggggatg cgcgtggaga ccgccgctgc tgaaacggcc
     3901 cggcccgccg ccgcgcctcg ggcggttgct cccaccacgg ctcagcaagt ggaccgcttg
     3961 gtgcaagggc tgggaacgcc ttcgtccgtc tctcgtccga ccctgcccgg cctggggcag
     4021 tccccgtctc cggttttcgg tccggcggcc cggcccgctt cggtgggtcc gtctttgttg
     4081 gacatgggtg atttgcatca gccggccgcg ttcctccccg cggccgcggc gtcgccggct
     4141 ttctatccgg cccactggca gttggacgcg gtggttcagg gttggctgag cggggaagcg
     4201 gtgcactgtt tcatcggcga ggacggccgg gttcgtctga gcatctttca ggacgccaac
     4261 gggaacgggg tgcaggactc caacgaaaaa cccctcacgg agacggatcc cctgctcctg
     4321 gatcatctgg tcaatgccat cgggaagatg gatggcgccc gcatctacct ggacggtcag
     4381 acgctggtct ttgccgacaa gctcggccgg gagttcacgc ggctggatct ggtgaaattg
     4441 gccggccaga ccgagcgcgg gaaacccacc gcggaggcga ccaaggccat ggccatcctc
     4501 ggtttttacg ccggattggc gctgcagtcc acggtgaacg ccgacgggac cgtgacgttg
     4561 cggattttgg atgagaacgg gaaaccggtg accacgccgc agacggtgga tctggacacg
     4621 ttcttgaaga cccaggtggc catcagccag ttgggaggga cgtcggaatt tttgaacgac
     4681 cgcaccatcg tttatcgcga cgccaacgga aaagaatact cccgcgtgga tctggtggtg
     4741 ctggcgggca actcctccaa aggcgcggag gcggcggcca tcctgggctg gtatgccggg
     4801 ctgtcgttga actccaccgt cacccccgaa ggcaaagtga tgatgaaaat cggcggccaa
     4861 acgccggtgg aggtggacct ggacacgttc ctgcgcacgc agacggccat cagcgagttg
     4921 gccggttcaa aggagttcct gaacgacggg accatcgttt atcgcgaccg caccggcaag
     4981 ggatattccc gcgtggactt gttgcggctg gcgggcacct cttccaaagg cgcggaggcg
     5041 gcggccatcc tgggctggta tgccgggctg gcgttgaact ccaccgtcac ccccgaaggc
     5101 aaggtgatga tgtccatcgg cggccaaacg ccggtggagg tggacctgga cacgttcttg
     5161 cgcacccagg cgaagatcag cgaactggcc ggcgcccagg cctttttgga cgataagagc
     5221 atcgtcttca agagcggcgg gaaatcctat tcgcgggtgg acctgctcat cgcttccggc
     5281 aaggcgacgc ccgaaggact gaaggccgcg gcggtgctgg gctggtacgg cgggctggcg
     5341 ctccagtcga ccatgaagga cgggaaggta ttcctgcaga tcaccgacgc cgacgggaac
     5401 cggttgccca tcccggaatc ggtggacctc gatacgttcc tgagaatgca ggtggccatc
     5461 agcgaactgg gcggggccaa agagttcctg gacgaccgca cgatcgtttt caaggaccgg
     5521 aacggccagg aatttacgcg ggtggatctg gttcagatgg cgggaacgcc ggaaggggtc
     5581 caagcggcgg ccatcctggg ctggtacgcg gggttggcgc tcaacgccac catcaccgag
     5641 gacgggaagg tgcggctgaa catcggcggc gcggtgccgg cggaggtgga cctggacacg
     5701 tatttgatga cgcaggcgaa gatcagcgaa ctggccggcg ccaaggcgtt catcaacggc
     5761 accgccatcg ttttcgtcga tgcggccggc cgggaatatt cgagactcga cctgctcacc
     5821 gaggccggga aactgactcc cgaaggaacg cgcgccgcgg cggtgttggg gtggtacgcc
     5881 ggactggcgc tgaggtgcac cgtgagcggc ggccgggtga cgctgcatat caccgacgag
     5941 aacgggaacg ctctccccac gccgg
//
LOCUS       DAOTFD010000163         5910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9641_length_5910_cov_4.450555, whole genome shotgun sequence.
ACCESSION   DAOTFD010000163 DAOTFD010000000
VERSION     DAOTFD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_5910_cov_4.450555"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1364)
                     /locus_tag="P5079_08835"
     CDS             complement(102..1364)
                     /locus_tag="P5079_08835"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRY30124.1"
                     /translation="MIQCARKLKQLPPYLFTRIKALTAQAYAKKLDVIDLGMGNPDVP
                     TPPHVVERLMDTVNNHPRTHRYPQAKGMPRFRKAVVKHMERRFGVGLDPDSEVLALVG
                     SKEGIAHLCAAYLNPGDVALVPVPAYPVHFNGVILAGGKVQPVPLRPENGYLPDYGKI
                     PRRVLERAKLIFLNYPNNPTAAVVEDLAFFKETVRFAKKNDLLVAYDNPYCEITFDGY
                     KAPSFLETPGARDVALEFHSFSKTYNMAGWRMGWVCGKKELLAPLEKFKSFVDYGVPT
                     FLQLAGAAALEGSQECVKQTVEVYRRRRDYFVNELNRMGWAVPMPKATMYVWAPLPEP
                     FRAMGSLAFAEKLVLETGIAVAPGVGFGQQGEGYVRFALVTHDNRFYDAALRIKKFFR
                     KHGALPARPAAAREERRPRPRPVEAETR"
     gene            complement(1407..1703)
                     /locus_tag="P5079_08840"
     CDS             complement(1407..1703)
                     /locus_tag="P5079_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30125.1"
                     /translation="MTEKVAVRALPRLAAVLFMGWGTLFFIKGMWDCFVGGPEANFYS
                     SRPWEFVTREQWFRFAGFEAFYGAAVFFLGRALWIFSKRLPAWREREKSSFEPI"
     gene            complement(1764..2039)
                     /locus_tag="P5079_08845"
     CDS             complement(1764..2039)
                     /locus_tag="P5079_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY30126.1"
                     /translation="MNKSQLILKISKDAGLRRAQSVRAVKALVGSIRETIKNGGKISL
                     TGLGTFKVKARKARPGRNPKTGETIAIPAGRKISFKPSLSLKKLIKA"
     gene            complement(2222..3310)
                     /locus_tag="P5079_08850"
     CDS             complement(2222..3310)
                     /locus_tag="P5079_08850"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRY30127.1"
                     /translation="MTKEKSSKNTSGVLRNPAKGKEFDAWDPKVLRRIDELVALADVP
                     GAVQDNRDLVREIIVTALKTHHSQIDRGDVKILSRSLREMRYGFRVFKEYRHLRKVTM
                     FGSARTPEGHAEYQQARQFARRMSKNGYMVITGGGPGIMEAGNEGAGPGKSFGINIRL
                     PFEQGANKYIATQPDLYIDCRFFFTRKLFFLKETSAAVFFPGGFGTLDEAMEVLTLVQ
                     TGKSDPMPIVFISPSGSRYWSDLKHYMEKHLLKTGKILPADLSLFYVTNSVSAAAAEI
                     LRFYKNYHSKRYVGDDLVIRLQRPLPPAGLKKITERFADICQKGGFHLGGALPEENEY
                     PDLPRLVFRFKRMRFGRLRQLIDVLNTY"
     gene            3467..5125
                     /locus_tag="P5079_08855"
     CDS             3467..5125
                     /locus_tag="P5079_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRY30128.1"
                     /translation="MRSVAAARPSPSVSSFPARILFLSALFLGAAASAPLLFAKDFAW
                     SQKNLRAGQVSRRGLRAGAINGKLYVVGGASAATPVDTVEEYDPGADAWSAKTPLLSA
                     KTEFGVAVIGNKLYVVGGDPLANPSLQMYDPLADAWTSKANMITPRKYLAVCTVNNKI
                     YAFGGSTSGGVTNLVEVYSPDIDGWSPKANLPVPRAHATACAYGNRIFLFGGEAVTDG
                     YVYNVDEYDTVADTWTTRSFLDARNDAVALTLEDKIYLFGGRKNANTLSTVEEYDPSQ
                     DRFTAKISLPAPRAQHAVAAVDNKVYVIEGGKSAVDEADSLVYRGILTGVPALSATGE
                     PHYTTDGLHPETGDSQTVFLFRIKYTDDDNEPPIAGYPRLHLRKGGAEIPGSPFSMTA
                     ANSADGVYTDGKIYSHALSGLTVGADYAHFFEAYDLNGSTAVGMLTTATAAPEVPEGI
                     TTRPPLQTDFHSTLYNNVFNPRQGGSCTIVFSVPQPGNVRLRLYDLLGREVRTLLDQP
                     EPAGDFQKIWDGRNADNSFVPPGLYLLHYDYPGGREVRKIAVKG"
     gene            5122..>5910
                     /locus_tag="P5079_08860"
     CDS             5122..>5910
                     /locus_tag="P5079_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HRY30129.1"
                     /translation="MKKRLAVFGLVLLSASALRAGQSGGAFLELPSSVRAGGMAETFT
                     ALADEPFGLNYNPAGIAFVPSPMASFLHQVYVQDVTAEFLGFVQPLGRVSLAAAPALL
                     SMKEEPVYDAQGAPTGATFQYEGTLLPGGVSYRKNNSAAGAVVKYYSEKIAGFSQNAT
                     TLDVGTLHRISNYRFGAAGQNLVGRLGDYDLPRTLRLGAAYEHKFVTVAVDAGRQLVQ
                     GKNIFSAGAEFAATAHAALRTGYRSQEFGGLSAGIGLKWPPLVVD"
BASE COUNT         1109 a         2012 c         1716 g         1073 t
ORIGIN      
        1 cgccttgctc tccaacacgg gcaggttggt ctgcaggagt ttggccaccc ccagcccgac
       61 ggtgccgcag ccgacgaggc cgatgttgac gacgtctttc ttcatcgggt ttccgcctcc
      121 acgggacgcg gtctcgggcg gcgctcctct cgggcggcgg cgggacgggc gggaagggcg
      181 ccgtgcttcc ggaaaaattt cttgatccgc agggcggcgt cgtagaaacg gttgtcgtgg
      241 gtgacgagcg cgaaccgcac atagccctcc ccctgctggc cgaacccgac gcccggcgcc
      301 acggcgatgc cggtttcgag caccagcttc tcggcgaagg ccagcgaacc catggcgcgg
      361 aaaggttccg gaaggggcgc ccagacgtac atggtggctt tcggcatggg cacggcccag
      421 cccatgcggt tcagctcgtt gacgaaatag tcgcggcggc ggcgatagac ctccaccgtc
      481 tgtttgacgc actcctgcga tccctccagg gcggccgcgc cggccagctg aaggaacgtg
      541 ggcacgccgt aatccacgaa ggacttgaat ttttccagcg gcgcgaggag ttcctttttt
      601 ccgcagaccc atcccatgcg ccagccggcc atgttgtagg tcttggagaa ggagtggaac
      661 tccaaagcca cgtcgcgcgc gccgggcgtt tccaggaaac tcggcgcctt gtacccgtcg
      721 aaggtgatct cgcagtaggg gttgtcgtac gccaccagaa ggtcgttctt cttggcgaaa
      781 cgcaccgtct ccttgaagaa ggcgaggtcc tccaccacgg ccgcggtggg gttgttgggg
      841 taattcagaa agataagttt ggcgcgctcc aaaacgcgcc gcgggatctt cccgtaatcg
      901 ggcagatagc cgttctccgg cctgagcggc accggctgca ccttcccgcc ggcgaggatc
      961 accccgttga aatgcacggg gtacgccggc accggcacca aggccacgtc gccggggttg
     1021 agataagcgg cgcaaagatg cgcgatgccc tctttggagc cgaccagcgc cagcacctcg
     1081 ctgtccgggt cgagccccac gccgaaccgc cgttccatgt gcttcaccac ggccttgcgg
     1141 aagcggggca tgcctttggc ctgcggatag cggtgcgtgc gcggatgatt gttcacggtg
     1201 tccatcagcc gctccacgac gtgcggcggg gtggggacgt ccggatttcc catccccagg
     1261 tcgatcacgt ccagcttttt ggcgtacgcc tgggccgtga gggccttgat gcgggtgaac
     1321 aaataaggag gaagctgttt gagtttacgt gcgcactgga tcatttttca attccgtttc
     1381 ccggcgccgc cgcggcgccg cacatgtcaa atcggttcga aggaactctt ctcccgttcc
     1441 cgccaggccg gcaggcgttt cgaaaaaatc cacaaagccc gtccaagaaa aaagacggcc
     1501 gccccgtaga acgcctcgaa accggcgaag cggaaccact gttcccgcgt gacgaattcc
     1561 caggggcggg acgaataaaa attggcttcc gggccgccga cgaaacaatc ccacatcccc
     1621 ttgataaaga agagagttcc ccaccccata aaaagaacgg ccgccaaacg cggaagggcg
     1681 cggactgcca ctttttcagt catcgatgga aaccctcaag gcccgacgtg gaaacttctc
     1741 cccccgcaaa cgcttccgga gggtcaggcc ttgatcagct ttttcagcga caggctcggc
     1801 ttgaaagaaa tctttcggcc cgccggaatg gcgatggttt ccccggtttt gggattgcgc
     1861 cccggccggg cctttcgggc cttcaccttg aacgtcccca gcccggtgag ggaaatcttc
     1921 ccgccgttct tgatggtttc ccgaatggac cccaccagcg ccttcacggc gcggaccgac
     1981 tgggcccggc gcaaaccggc gtctttggat atcttcagga tcaactgcga tttattcatg
     2041 agcacaacct ccggaaaatc gtcgcaccag ccttacaacc cgcgaccggt cctgtccata
     2101 attcgattta tatctatttt tagctgagaa aatgcatttt gtcaagggca tcaaaaacac
     2161 gggctttgcc gcggacagcc cccccggccg ccgcatttaa aggccccccc gaaaaagctc
     2221 cttaataggt attcaaaacg tcgatgagct gccgcagccg gccgaaccgc attctcttga
     2281 accggaagac gagccgcggg aggtccggat attcgttctc ttccggcaag gcgccgccga
     2341 gatggaaccc ccctttctgg cagatatcgg cgaaccgctc cgtgatcttt ttcaggccgg
     2401 ccggcggaag gggccgctgc aggcggatca ccagatcgtc ccccacgtaa cgcttggagt
     2461 gatagttctt gtaaaaacgg aggatctccg cggcggcggc ggacacggaa ttcgtcacgt
     2521 agaagaggct caggtcagcc ggcaatattt tcccggtctt caacagatgt ttctccatat
     2581 aatgtttcaa gtcggaccaa tagcggctgc ccgacgggct gatgaacacg atcggcatgg
     2641 ggtcggactt gcccgtctgc accagggtga ggacctccat cgcctcgtcg agggtgccga
     2701 accctcccgg gaaaaagacc gcggcgctgg tctctttcag gaaaaacagt ttccgggtga
     2761 agaagaaccg gcagtcgatg tagaggtccg gctgagtggc gatgtatttg ttggcgccct
     2821 gctcgaacgg aagccgaatg ttgatgccga aactcttccc cggaccggcg ccttcgttgc
     2881 ccgcctccat gatccccggc ccaccgcccg tgatcaccat gtagccgttc ttcgacatcc
     2941 gccgcgcgaa ttgacgggcc tgctggtatt cggcgtgccc ttcgggggta cgggcggacc
     3001 cgaacatcgt caccttccgc aaatgccggt attccttgaa cacccgaaac ccgtaacgca
     3061 tctcccgcag ggaacggctg aggatcttca cgtcgccgcg gtcgatctgc gagtggtgcg
     3121 tcttcaacgc ggtgacgatg atctcccgca ccaggtcgcg gttgtcctgc acggcgccgg
     3181 ggacgtccgc cagcgccacc agctcgtcga tgcgccggag gaccttcggg tcccacgcgt
     3241 cgaactcttt ccccttcgcc gggttccgaa gaacgcccga ggtgtttttg gaggatttct
     3301 ctttcgtcat gccggtttga ttttcccttt caaaaacgaa aaaacggcgc gcggacggat
     3361 cgcgaccgtt ctttttccat tatacacaat ttgcggccgc tcccctctcc cggcggcgac
     3421 ggaccacccc cggccggaat tggggcttat ttgctaatat cccctcatgc gctccgtcgc
     3481 ggcggcaaga ccgagcccct ccgtctcttc ctttccggca cggatactct ttttatccgc
     3541 gctgttcctc ggcgcggcgg cctccgcgcc cctcctcttc gccaaagatt tcgcttggtc
     3601 ccaaaaaaac ctccgcgccg gacaggtctc ccgacggggg ctccgggcgg gcgccatcaa
     3661 cggcaaactc tacgtcgtcg gcggggcctc cgccgccacg cccgtggaca ccgtggagga
     3721 atacgacccc ggcgccgatg cctggagcgc gaaaaccccc ctcctctccg ccaaaacgga
     3781 gttcggcgtg gcggtcatcg gcaacaaact ctacgtcgtc ggaggagacc ccctcgccaa
     3841 cccctccctg caaatgtatg accccctcgc cgacgcgtgg acatccaaag ccaacatgat
     3901 caccccccgg aaatacctgg ccgtctgcac ggtgaacaac aagatctacg ccttcggcgg
     3961 gtccacctcc ggcggggtga cgaacctcgt cgaggtctac tccccggaca tcgacggctg
     4021 gagcccgaag gccaacctgc ccgtcccgcg ggcgcacgcg accgcctgcg cctacggcaa
     4081 caggatcttc ctgttcggag gagaagccgt caccgacggc tacgtctata acgtggacga
     4141 gtacgacacg gtcgccgata cttggaccac ccggtctttt ctggacgccc ggaacgacgc
     4201 cgtcgcgctg accctggaag acaagatcta cctcttcggc ggacggaaaa acgcgaacac
     4261 cctttccacc gtggaggaat acgacccctc ccaggaccgg ttcaccgcga agatatcgct
     4321 ccccgcgccg cgggcccagc acgccgtcgc ggccgtcgac aacaaagtct acgtgatcga
     4381 gggcgggaaa agcgccgtcg acgaggcgga cagcctggtc tatcggggca tcttgaccgg
     4441 cgtgcccgcc ctgtcggcca ccggggaacc ccactacacc accgacggcc tgcacccgga
     4501 aacgggcgac tcccagaccg tcttcctctt ccgcatcaaa tacaccgacg atgacaacga
     4561 gccgcccatc gccggatatc cccgcctgca cctccgcaaa ggaggcgcgg aaatccccgg
     4621 cagccccttc tccatgaccg ccgccaattc cgccgacggc gtctacaccg acggcaaaat
     4681 ttactcccac gccctctccg gattgacggt cggcgccgat tacgcccatt tcttcgaggc
     4741 ctacgacctc aacggcagca ccgccgtcgg catgctcacc accgccaccg cggcgccgga
     4801 ggtgcccgag gggatcacga cccggccgcc cctccagacg gatttccact ccaccctcta
     4861 caacaacgtc ttcaatcccc gccagggcgg ttcctgcacc atcgttttca gcgtgcccca
     4921 gccggggaac gtgcggctgc gcctctacga cctgctcggg agggaggtcc ggaccctgct
     4981 ggaccagccc gaaccggccg gcgatttcca gaaaatatgg gacggccgca acgcggacaa
     5041 cagcttcgtt ccgccgggac tgtatctttt gcattacgac taccccggcg gccgcgaagt
     5101 gcgaaagatc gccgtcaagg gatgaaaaaa agactcgccg ttttcgggct cgttctcttg
     5161 tccgcctccg cgctccgtgc cggacagagc ggaggcgcgt tcctggaact cccctcctcg
     5221 gtccgcgccg gcggcatggc ggagaccttc accgcgctcg ccgacgagcc gttcggcctg
     5281 aattacaacc ccgccgggat cgctttcgtt ccttcgccga tggcctcgtt cctccaccag
     5341 gtttacgtgc aggacgtcac cgccgagttc ctcggattcg tccagcccct gggccgcgtt
     5401 tccctggccg ccgcgccggc ccttctctcc atgaaagaag aacccgtcta cgacgcccag
     5461 ggggccccca ccggcgccac gtttcaatat gaggggacgc tgttgccggg cggcgtctcc
     5521 tatcgcaaga acaattccgc cgcgggagcc gtcgtcaaat attactcgga aaagatcgcc
     5581 gggttcagcc aaaacgccac cacgctggac gtcgggaccc tccaccggat ctccaattac
     5641 cggttcggcg cggcgggaca aaacctcgtc ggccggttgg gcgattacga cctcccccgc
     5701 accctgcgtc tcggcgcggc ctacgaacat aaattcgtga cggtcgccgt cgacgccggc
     5761 cggcaattgg ttcaaggaaa aaacattttc agcgccggcg cggagttcgc cgccaccgcc
     5821 cacgccgcct tgcggaccgg ataccggtcc caggaattcg gcgggctgtc cgcggggatc
     5881 ggcctgaaat ggccgccgct cgtggtggac
//
LOCUS       DAOTFD010000164         5902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9657_length_5902_cov_3.713357, whole genome shotgun sequence.
ACCESSION   DAOTFD010000164 DAOTFD010000000
VERSION     DAOTFD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_5902_cov_3.713357"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5902
                     /locus_tag="P5079_08865"
     CDS             <1..>5902
                     /locus_tag="P5079_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30130.1"
                     /translation="EPGVMHKSGKGGGEYVGQKTTTSTTTLSDFDQYGRPHSTYTSYR
                     SSDGSWGWSRITNMVYNNLGLVVSQHEDYYSYTANVAKNTDQTVRGSKDVTFAYDALN
                     QLISTRDSNVHETSSTEQRGCTKFMNSWIGKVIKLVIDIVLTMTGVGILVVFAINFLW
                     TYIATGSLKMALIAGVMSAATAGVGASGLDTWVKVILNATIAVCGTYLATGSWKAALG
                     AGLGAAVGTALSSSIGGTTGAIVGGVVGGAVAFGVTYALTGSTRMAIIAALNAAIVVI
                     ADLFRETGKDSGGLFKTDVGKQVGGIGTFFKELFKSTSGAQFQTFMKEFIRGVISITV
                     EQLLPQDLKFMATALGALVSAAVGKMLVSMGGDAAKIGLGLMGGKEISWGGIIKDMAK
                     QTSVAVVGWAAGKWLGAGKKEGDNSFLVPIITSLASAVLSGEKLTFKYLAHMLAGGLV
                     KAMAEYNIVQMQNLFPEIALNSSINSMFGDWMRAPANVISSGLEKGAILKGDTVIVLR
                     PESEVTLPGGETLVFSEEGEYELPLDSEGRLDAESQGRSSDGRTIAAVVRKGDKEVLV
                     FSSEFERGVEEGSLEVTEEAQEAPMSSVGQAAGQSVETAEESAGEKKAAPAAGSATEG
                     EGGTRKWTVQGTLSGGQEFRMEFFTTNQKIVQQQFFVATTDFSSVEAVFIYNQQSQKF
                     EAYHENQKFNVYFNNQKLEVQVGRGDDGKLHLYLTVSRDEMAIQLKETFGQDFDTKQF
                     FAAIDELLAPGALDKGIMLQVDFNANGEMELSFTAKFSDLSKTAQAVLKAFNAVNAEG
                     NPLSLDDKVKIVYRDGKAYMDFNFKSSAADIKAALSQLVDQGVYTKEEAAKVQEHLDT
                     YLQQDSKALLDGHAQLTVKGEGIIELKVNASNLTALSKEAQTWLREAGTNEAKLVFTM
                     ARDKAGKLTVQSHMEFKVASENKATFFKFLASQDNAAVNKVADLFGKTFGVSNLLEDA
                     QNILSGVDLEIEADLQAMAGKDGTKKDDPLKIKAELLLNVLLQRIQAGVPINVNDALF
                     KTMVDAKLGDAKIKLVFSAAADQPILEDAKFSSSALPMLIAKCPPNSELHQTLAGMRK
                     FSQGKTQTIEMHRGTDGKQRFFVSLDVSELPAGVSVDSKGPMPTHGPVRLMWTAGAQR
                     PVLAAGGQVALHKEVVMKDFRLFEGMAGAPLQEIKVKVTEIVLGRDRLGRDVIQFILA
                     EPFAEGLPREDQTVSGQGNLAVNGFKGETKAKILGVTTIIATRDGKESWGAGYRLRLS
                     GDMELSRETEGYKYRVLQGAVVELMGGNIGVVDGNVRYEATRATEIRMANGSTLMLKK
                     AGDFVEMGAHGITDCTAGTVIRAADGKSQQILLGREGDSSAMRVRYESIGNQTAEITL
                     GDGSTLVLRAGEYVVMGAKGIEDFKAGVEIVSADKKYKQVLMERVGAAIRTRFEALTA
                     GAVISLSGGTYLILKGVGDSVVIGEKGFESWKGTVLVARGGVCSQIMMDHPALGKVTL
                     LLSETSTSQVLGGKFNVLMVVAGDGRQLQLEKAVEVDLTLSGLGEGKVGVYRDAKGNA
                     LFLEDTKVEQALKGRGNVKGVQVPTAKEGESQQLMFERTQSNAELNNLDVSILVATSR
                     RVDASGNAIGNTLFLETTTMAQALQGKGNVKGVQTPTAQKGEFRQVFFGTTQFNANLE
                     SLDTSAAAVSYRRVDAEGHALGNTVFLEKTTIAQALNGRGTAIGVQGPDGRRVMFVRA
                     QKEADLDALKYTAAAASYQRFNENGEAVGPVIFLTKTSVAQALKGIGSAMGVQSGQRQ
                     VMFAVTQWGVNLDAVNLRAAAASYQRVDGRGRPVGAAVFLTDTTAREALKGSGTAIGV
                     QNRDGQRVMFAKSQKTANLDNLDYDAGAASYQKFDGAGKPEGNAVFLQGTNIRDALRG
                     RGTAVGMETPDGRRVMFSAPQATENLNALNLQASAATYQLFKDQKPAGVLVLLTNTSI
                     EQALSGRGT"
BASE COUNT         1306 a         1450 c         1981 g         1165 t
ORIGIN      
        1 ccgagccggg ggtgatgcac aagagcggca agggcggcgg agaatacgtg gggcagaaga
       61 ccaccacctc cacgaccact ttgtccgatt tcgaccagta cggccgcccc cattcgacct
      121 acacctccta tcgcagttcc gacgggagtt ggggttggag ccgcatcacg aacatggtgt
      181 acaacaatct gggcttggtg gtttcccaac atgaagacta ttacagttac accgccaacg
      241 tcgccaaaaa taccgaccag accgttagag gctccaagga cgtaacgttc gcctacgacg
      301 cactcaatca actcatttcc actcgcgaca gcaacgttca cgagaccagc agcaccgaac
      361 agcgcggttg cacgaaattc atgaattcgt ggatcgggaa ggtcatcaag ctggtgatcg
      421 acatcgtgct gaccatgacg ggggtgggga tactcgtcgt tttcgccatc aatttcctct
      481 ggacctacat cgccacgggc agcctcaaga tggccctcat cgccggggtc atgtcggccg
      541 ccacggccgg ggtgggggcc tcggggctcg acacctgggt caaagtgatt ctcaacgcca
      601 ccatcgcggt gtgcggcacc tatctggcca cgggcagttg gaaggcggcg ttgggggcgg
      661 ggctcggggc ggcggtcggc acggcgcttt cttccagcat cggcggaacc acgggggcca
      721 tcgtgggcgg cgttgtgggc ggggcggtgg cgttcggggt cacctatgcg ctcaccggca
      781 gtacgcgaat ggccatcatc gccgcgttga acgccgccat tgtggtgatc gccgatcttt
      841 tcagggagac ggggaaagat tccggcggtt tgtttaaaac ggatgttggg aagcaggtcg
      901 gcgggatcgg caccttcttc aaggaacttt tcaagtcaac atccggcgct cagttccaga
      961 cgttcatgaa ggagttcatc cggggcgtca tcagcatcac cgtcgaacaa ctcctgccgc
     1021 aagacctgaa gtttatggcc accgccctcg gcgccctcgt ctcggccgcc gtcgggaaga
     1081 tgttggtctc catgggcggc gatgctgcga aaatcggcct gggcttgatg ggagggaagg
     1141 aaatttcctg gggaggcatc ataaaagaca tggccaaaca gaccagtgtg gcggtggtgg
     1201 gctgggcggc cgggaagtgg ctgggcgcgg gcaaaaagga gggggacaat tcgttcctgg
     1261 tgccgatcat caccagcctg gcctcggccg tcctctccgg ggaaaaactg actttcaagt
     1321 atctggcgca tatgctcgcg gggggcctcg tgaaggcgat ggcggagtac aacatcgtgc
     1381 agatgcaaaa cctgttcccg gagattgcgc tgaattcatc cattaattcc atgttcggcg
     1441 attggatgag ggctcccgcc aacgtgatct cttccggcct ggagaagggg gctatcctta
     1501 aaggggacac ggtgatcgtg ctgaggccgg aatcggaggt caccttgccg ggcggggaga
     1561 ctctggtgtt cagcgaagag ggggaatatg aattgccgct cgattcggag gggagactcg
     1621 atgcggaaag ccaggggcgt tcctccgatg gccgaacgat cgcggcggtg gtgcggaaag
     1681 gagacaaaga ggttctggtg ttttcgagtg aatttgaaag gggcgtcgaa gaaggctcct
     1741 tggaggtcac ggaggaggcg caggaagcgc ccatgtcctc cgtcggccag gcggccggcc
     1801 aatccgtcga aacggcggag gaatcggcgg gcgagaagaa ggccgcgccg gcggccggct
     1861 cggctacgga aggcgaggga gggacgcgca aatggaccgt gcagggaacg ctctcggggg
     1921 ggcaggagtt ccggatggaa ttctttacga ccaatcaaaa aatcgttcaa caacagtttt
     1981 ttgtggcgac aacggacttc agttccgtgg aagcggtttt catctataac cagcaatcac
     2041 agaagttcga ggcctatcac gaaaatcaga agttcaacgt ctatttcaac aatcaaaaac
     2101 tggaggtgca ggtgggccgg ggagacgacg gcaaactaca cctctatctg acggtttccc
     2161 gggatgagat ggcgatccag ttgaaggaaa ctttcgggca ggacttcgac acgaaacaat
     2221 tctttgcggc catcgatgag ttgttggcgc ccggggcttt ggataagggc atcatgctgc
     2281 aggtcgattt taatgccaat ggggaaatgg aattgtcgtt taccgctaaa ttttccgacc
     2341 tcagcaagac cgctcaggcg gtgttgaagg cgttcaacgc ggtgaatgcc gaagggaatc
     2401 cgttgagcct ggacgataag gtcaagatcg tttatcgcga cggcaaggcg tatatggatt
     2461 tcaacttcaa atcttccgcc gccgacatca aagccgcgct cagtcaactg gtggatcagg
     2521 gggtctatac caaggaggag gcggcgaagg ttcaggagca cctcgatacc tatctgcaac
     2581 aggattcaaa ggccctcctc gacgggcatg cccaattaac ggtgaaaggc gaaggaatca
     2641 tcgaattaaa ggtcaatgcg tccaatctca cggcgctctc caaggaggct cagacctggc
     2701 ttcgggaggc cgggacgaac gaggccaagc ttgtttttac catggcgcgc gacaaggccg
     2761 gcaaattgac ggtccagtcg cacatggagt tcaaggtcgc ctccgaaaat aaagccacct
     2821 tttttaagtt tttggcgagc caggataacg ccgcggtgaa caaggtggcg gacttgttcg
     2881 gaaaaacttt cggcgtctcc aatttgttgg aggatgccca gaatatcctg tccggcgtcg
     2941 atctggagat cgaggcggat cttcaggcca tggcgggcaa agacgggaca aagaaggatg
     3001 atccgctgaa gatcaaggcg gaattgctgt tgaatgttct cttgcagcgg attcaggccg
     3061 gagttcccat caacgtcaac gatgccctct ttaagaccat ggtggacgcc aagctggggg
     3121 atgccaagat caaactggtg ttcagcgccg ccgccgacca gccgatcctg gaggacgcga
     3181 aattcagctc ctccgcgctt cccatgctga tcgccaaatg ccctccgaac agcgagctcc
     3241 atcagaccct ggcggggatg cggaaattct cgcaggggaa aacccagacg atagaaatgc
     3301 accgggggac ggacggcaag cagaggttct tcgtctccct ggatgtgtcg gagttgcccg
     3361 ccggggtttc ggtcgattcg aaggggccca tgccgacgca tggtcccgtc aggcttatgt
     3421 ggacggccgg cgcgcaacgg cctgttctcg ccgcgggcgg ccaagtcgca ttgcataagg
     3481 aagtggtcat gaaggatttc cggctcttcg aggggatggc cggggccccg cttcaggaaa
     3541 taaaagtgaa agtcaccgaa atcgttttag ggcgggatcg cttgggtcgc gacgttattc
     3601 agttcatctt ggcggagccg ttcgcggagg ggcttcccag ggaagatcaa acggtctccg
     3661 gacaggggaa cctggcggtc aacggcttta aaggagagac gaaggcgaag atcttgggag
     3721 tcacgacgat catcgccacc cgggacggca aggaatcttg gggggcgggc tatcggctcc
     3781 gtctttcggg ggatatggag ctctcgcggg agacggaagg atacaaatac cgagttctcc
     3841 aaggcgccgt ggtcgaactc atgggcggca atatcggcgt tgtcgacgga aatgttcgat
     3901 acgaagctac ccgtgcgacg gaaatcagga tggccaatgg ctccacgttg atgttaaaga
     3961 aggccggtga ttttgtggag atgggggccc acggaatcac ggattgcacg gcggggacgg
     4021 tgatccgggc ggccgacggt aaatcccaac agatattgtt gggccgggaa ggggattcca
     4081 gtgcgatgag agtccgttat gagtccattg ggaatcaaac ggcggaaatc acattgggag
     4141 acggctcgac gctggtcctt cgggcgggtg agtatgtcgt gatgggggcg aaggggatag
     4201 aggactttaa ggccggggtg gagatcgttt ccgccgataa gaaatacaag caggttctga
     4261 tggagcgggt cggggcggcc attcggacgc ggttcgaggc gttgacggcc ggggcggtca
     4321 tttctctcag cggggggact tatctgatcc tgaagggcgt cggggattcc gtcgtcatag
     4381 gtgaaaaagg ttttgagtcg tggaaaggca cggtgctggt cgctcgcggc ggggtctgct
     4441 cccaaatcat gatggatcat cccgctttgg gaaaagtgac gttgttgctc tccgaaactt
     4501 cgacaagcca ggtgctgggt ggaaaattta atgtgcttat ggtcgttgcc ggggacgggc
     4561 gtcaattgca actcgaaaaa gcggtggagg tcgatttaac gttgtccgga ttgggcgaag
     4621 ggaaggtcgg cgtttatcgc gacgcgaagg ggaacgcctt gttcttggaa gacacaaaag
     4681 tcgaacaggc cctgaagggg cgggggaatg tgaagggcgt gcaggtgccg accgccaaag
     4741 aaggggagtc ccagcaattg atgtttgagc ggacgcagtc gaacgcggaa ttgaacaact
     4801 tggatgtttc catattggtg gcgacgtccc gaagggttga cgcttccggg aatgcgatcg
     4861 gcaatacgct gtttttggaa acgacaacaa tggcgcaggc cttgcagggg aaagggaatg
     4921 tgaagggcgt gcagacgccc acggcccaga aaggggagtt ccggcaggta ttttttggaa
     4981 cgacgcagtt caatgccaac ctggagtccc tcgatacgtc cgccgcggcg gtcagctatc
     5041 ggcgggttga cgcggaaggt cacgcgcttg ggaataccgt ttttctggaa aaaacaacga
     5101 tcgcgcaggc tttgaatgga aggggaacgg ccatcggggt ccaaggaccg gacggccgtc
     5161 gtgtgatgtt cgttcgtgcg cagaaagaag cggatttgga tgcgttgaaa tatacggccg
     5221 ccgcggcgtc atatcagaga ttcaatgaga atggggaggc ggtcgggccg gtcattttct
     5281 tgacaaaaac atccgtggct caggcgttaa aggggattgg ttcggccatg ggggttcaga
     5341 gcggacagcg gcaagtcatg tttgcggtga ctcagtgggg cgtgaatctg gatgccgtga
     5401 atctgagggc ggccgccgcc agttatcaac gggtggatgg tcggggccgg cccgtcgggg
     5461 cggccgtttt cttgacggat acgacagcga gagaggcttt gaaggggagt ggcacggcga
     5521 tcggggttca gaatcgggat ggccagcgtg tcatgttcgc caagtcgcaa aagaccgcga
     5581 acctggataa tctggattac gacgcggggg cggcttctta tcagaaattt gacggggcgg
     5641 gaaaaccgga agggaatgcg gtgttcttgc agggcacgaa tatccgcgat gctttgaggg
     5701 gccggggcac ggccgttgga atggaaacgc ccgatgggcg acgtgtcatg ttttcggctc
     5761 cgcaagccac cgagaatttg aatgcgttga acctgcaggc gtcggcggcc acttatcagt
     5821 tgttcaagga tcagaaaccg gcgggggtcc ttgttcttct gacgaacacc tcgattgaac
     5881 aggcgttgtc cggtcggggg ac
//
LOCUS       DAOTFD010000165         5886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9698_length_5886_cov_4.159321, whole genome shotgun sequence.
ACCESSION   DAOTFD010000165 DAOTFD010000000
VERSION     DAOTFD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9698_length_5886_cov_4.159321"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..598
                     /locus_tag="P5079_08870"
     CDS             365..598
                     /locus_tag="P5079_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30131.1"
                     /translation="MTKPLLERLAEKSLEAAKQLTDAKKDRERLLAELELTREESRRA
                     RRILREYDELLKERKKVQQKLEQMLKKLDGLMV"
     gene            605..904
                     /locus_tag="P5079_08875"
     CDS             605..904
                     /locus_tag="P5079_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRY30132.1"
                     /translation="MRTLAAEKIRVRIFNRDYDIDPGGLTPLEASQLAAYVDQKMHEI
                     STHLHIVDTQKVAVLAALNIAFELSQEKGAAEKALSEDNTKKIEDILASLEKALK"
     gene            957..2294
                     /locus_tag="P5079_08880"
     CDS             957..2294
                     /locus_tag="P5079_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRY30133.1"
                     /translation="MDLFAAGKPAEQTNPRNDPLALRMAPRDWNEFVGQNHLVGEGSL
                     LRRAVEADRLGSAVFFGPPGTGKTALARLIAYKTKAHVEHTNAVTIGVPEIRKILQTA
                     HERRRAQGRRSLLILDEIHHFNRSQQDALLPDVEKGVITLVGLTTENPFFYVNAALLS
                     RSTVFEFFPLKNEDLRQILRWSLENKERGLGDRKILLSPEAETHFLETSGGDARRVLN
                     ALELAVLSTAPSRDGAVALTLAVAEASTQKRAVRYDKSSDDHYDTISAFIKSLRGSDP
                     DAALYWMGKMLAAGEDPRFIARRLLIAASEDVGNADPLALLVANAAFDAVEKLGLPEG
                     RIPLAQAVTYVASAPKSNAAYLAGDAAAGEVRQGPRREVPPHLRDANLDGAARGHGRG
                     YKYAHDFPEHFVKQEYLPLWKKFYFPTDLGFEKKIKERLERLWPERKTEKDKP"
     gene            2308..2886
                     /locus_tag="P5079_08885"
     CDS             2308..2886
                     /locus_tag="P5079_08885"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRY30134.1"
                     /translation="MPSEEILRHKSFLRSFFRHAVRSLSAAKRRQESRAVIRRAGKLP
                     VFQRARTVAVTLSRPWEIDTGPFLALCRKRGKRVVVPVVFPAGRRLRFVDLEPHPVLR
                     KNVHGILEPAAPSRTVPLKKIDLMVVPGAAFTPQKHRLGSGGGYYDRVLENFPASKTA
                     GFAFSVQLAPRLPVEPHDRRVGVLVTPRRLFR"
     gene            3011..3805
                     /locus_tag="P5079_08890"
     CDS             3011..3805
                     /locus_tag="P5079_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRY30135.1"
                     /translation="MRILFVGDIMGRAGREAIQHFIPRLRQEHRLDWVVANAENAAGG
                     RGVTPPIAQTLFQSGIDVITMGNHTWDRPEIESILADDRVIRPANYPPILAGHGDAVF
                     QVGGKTLGIVQVMGRHTLAEIDCPFRTMDAVLGKMKADVVLVDMHAEATSEKEAMGWY
                     LDGRVAAVLGTHTHVQTADERILPGGTAYITDAGMTGPRDSVIGGKIEIALKRFLTGI
                     PLRVDVADGDAQFNGCVLDLDEKTGKARSIERLFLLMERAKAEKPA"
     gene            3813..4739
                     /locus_tag="P5079_08895"
     CDS             3813..4739
                     /locus_tag="P5079_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY30136.1"
                     /translation="MPDSRLKKYLDKKRRTVDAALRRCLPKHAALPPVVHEAMRYSVF
                     AGGKRLRPILCLAAAELCGGTAKRVMPTACALELIHTYSLVHDDLPAMDDDDFRRGLP
                     TSHKKFGEGNAVLAGDALLTFGFQLMAENARRGRARPAAVLDVVRTVAEAAGSRGMVG
                     GQAADLAMSGNGKNRNGEKTPDALRKKLDYIHRNKTAALIRASLRAGALLAGASPVRL
                     NALDAYGRSIGLAFQIVDDILDIVGDKKLLGKRGSDRDNDKLTFPALYGLEASRRMAR
                     REVRRAKNALSLFGPRARILNDLADYIVERQN"
     gene            4756..>5886
                     /gene="dxs"
                     /locus_tag="P5079_08900"
     CDS             4756..>5886
                     /gene="dxs"
                     /locus_tag="P5079_08900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRY30137.1"
                     /translation="MAVLDMIEKPQDLRNIRRELLPDLAKEIRQKIVDVTAQTGGHLG
                     SSLGTVELIIALHYCFDSPKDKFVWDTGHQAYAHKLLTGRRDRFHTLRQFGGVSGFPL
                     RAESEHDHFGVGHASTSISAALGYACARDILKQDHRVVAVANDGSLTGGMAFEALQNA
                     GILGTNLLVVLNDNQMFISHRVGAFGAFLAKLLTLGSVKRMEKKVERFLTRINFWGAY
                     LLRLARRIKVFLFPGVLFEEMGFGYIGPVDGHNLNQLIDVITAVKQLTGPRVLHVVTK
                     KGRGYEPAEQDPVRYHGVSRFDPETGDMVKLEAGPPSYTKIFGQALVKLAKDDPKVVA
                     ITAAMPDGTGLDLFRKEFPDRFFDVGLGEQHAVTFAAGLACGG"
BASE COUNT         1176 a         1977 c         1742 g          991 t
ORIGIN      
        1 ccacgtagga cgcgaacgcc agcgccgcca ggcccgcgac gaaacccgcg gtagcgccga
       61 aggaaaattt gacgcccagg gccgcgcctc ccaccgccaa agcgctgacc gcccagccgt
      121 aggtctgccg catgagcaac ggttttttat cctgtgctat cggtgtcgag gggggcatat
      181 tttatccgtc gtccattata aaactttctt cgcggcggtc aaagaccttt tgcttcaaaa
      241 gaatccgttg cggcgcgggc cctctcctcc ggcccgccgg ggccgatttt cccggcctcc
      301 ttgaaacttg ataaaaagct ttaacttttt gcttttcccg tccgttttgt gctaatttct
      361 cttcatgacg aaaccccttc tggagcgttt ggcggagaaa agtctcgagg ccgcgaaaca
      421 gctgacggac gcgaaaaaag accgggaacg tctgctggcc gaactggaac tgacgcggga
      481 ggaaagccgc cgggcccgac ggatcctgcg ggaatacgac gagttgctga aggaacgaaa
      541 aaaagttcaa caaaagcttg aacaaatgct taaaaagctc gatggtttaa tggtttaatt
      601 acccatgcgc acattggcgg cggaaaagat tcgggtgcgg attttcaacc gggactacga
      661 catcgatccc ggcgggttga cccccctgga ggccagccag ctggccgcgt atgtggacca
      721 gaagatgcat gagatctcca cccatctcca catcgtggac acccagaaag tggcggtgct
      781 ggccgcgctg aacatcgcct tcgaactgtc ccaggaaaaa ggcgccgccg aaaaggccct
      841 ttccgaagac aacaccaaaa aaatcgagga catcctcgcc tccctcgaaa aagccctgaa
      901 gtagtcccgc ctccgacacc ctatttttac tccccattag tttgtaaaat tttcccatgg
      961 acctctttgc cgccggaaaa cccgccgaac aaacgaaccc ccggaacgat ccgctggcgc
     1021 tccgcatggc gccgcgcgac tggaacgagt tcgtcgggca aaaccatttg gtgggggaag
     1081 gcagcctcct gcgccgcgcc gtggaggccg accggctcgg ttccgccgtc ttcttcgggc
     1141 ctccgggcac cggcaagacc gccctggccc ggctcatcgc ctacaagacc aaggcccatg
     1201 tggagcacac caacgccgtc accatcggcg tgccggagat ccgaaaaatc cttcaaaccg
     1261 cccacgaacg ccgccgggcc caaggccgcc gatccctgct catcctcgac gaaatccacc
     1321 acttcaaccg cagtcaacag gacgccctcc ttccggacgt ggaaaaaggc gtcatcacgc
     1381 tcgtcggcct gacgacggag aacccgttct tctacgtgaa cgccgccctc ctctcccgct
     1441 ccaccgtgtt cgaattcttc cctttgaaga acgaagacct ccggcaaatc ctgcgctggt
     1501 ccctggagaa caaggaacgc ggcctgggag acagaaaaat cctgctctcg ccggaggcgg
     1561 aaacgcattt cctggagacg agcggggggg acgcccgacg ggtgctcaac gccctggaat
     1621 tggccgtgct cagcaccgcg ccgtcccggg acggggccgt cgccctcact ctcgccgtgg
     1681 ccgaggcctc cacccagaaa cgggccgtcc gctacgacaa atccagcgac gaccattacg
     1741 acaccatctc ggctttcatc aaatcgctgc gcggttccga ccccgacgcc gccctctact
     1801 ggatgggcaa gatgctggcc gcgggggaag acccgcgctt catcgcccgc cggctgctca
     1861 tcgccgcctc cgaggacgtg ggcaacgccg accccctggc gctcctggtg gccaacgccg
     1921 ccttcgacgc cgtggaaaaa ctgggactcc cggaaggccg catcccgctg gcgcaggccg
     1981 tcacctacgt ggcctccgcg cccaaatcca acgccgccta cctcgccgga gacgccgccg
     2041 cgggggaggt ccgccaggga ccccgccgcg aagttccgcc gcacctgcgg gacgccaacc
     2101 tcgacggcgc cgcccgagga cacggccggg gttataaata cgcccacgat ttccccgagc
     2161 atttcgtcaa acaggagtac ctgccgcttt ggaagaagtt ctacttcccg accgacctgg
     2221 gttttgagaa gaagataaaa gaacgccttg aacgtctctg gccggaacga aaaacggaaa
     2281 aagacaagcc ctgagcggtt ccccgacatg ccctccgaag agatcctgcg acacaaatcc
     2341 ttcctgcgaa gcttcttccg ccacgccgtc cggtccctgt cggccgcgaa acgccgacag
     2401 gaaagccggg ccgtcatccg acgggccggg aagctgcccg ttttccaaag agcccgcacc
     2461 gtggccgtga ccctctcccg cccctgggag atcgataccg ggcccttcct cgccctgtgc
     2521 cggaaaaggg ggaaacgcgt cgtcgtgccg gtggtctttc ccgccggccg ccgtctccgc
     2581 ttcgttgacc tcgagccgca tcccgtcttg cgaaaaaacg tccacggcat tttggaaccg
     2641 gccgctcctt cccggacggt ccccctaaaa aagatcgatc tcatggtggt tccgggggcg
     2701 gccttcaccc cccaaaaaca tcgcctggga tccggcggcg gttattacga ccgcgtcctg
     2761 gaaaatttcc ccgcgtccaa aaccgccggt ttcgcttttt ccgtccagct ggccccgcgc
     2821 cttcccgtcg aacctcacga ccgacgggtg ggggtcctcg ttaccccgcg ccgtcttttc
     2881 cgataacggc ttttcgcccg ccctctctct tcgcccgtgg gaatattttg tataatttgt
     2941 ttctatttca atatgtaact agcgccaacc gattcattcc gtcacgttcg atagaaagac
     3001 gaggtttccg atgcggattt tgtttgtggg cgatatcatg ggccgggcgg gacgggaagc
     3061 catccaacac ttcatccctc gtctccgcca ggaacaccga ttggactggg tggtggccaa
     3121 cgccgaaaac gccgccgggg gaaggggcgt gaccccgccc atcgcacaga ccctcttcca
     3181 atccggcatc gacgtcatca ccatggggaa ccacacctgg gaccgtccgg agatcgagtc
     3241 catcctggcc gacgaccggg tgatccggcc tgccaactat ccgccgatcc tcgcgggcca
     3301 cggcgacgcg gtctttcagg tcggcgggaa aaccctggga atcgtgcagg tgatggggcg
     3361 ccataccctg gcggagatcg actgcccgtt ccgaacgatg gacgcggttt tggggaagat
     3421 gaaggcggac gtggtcctgg tggacatgca cgccgaggcg accagcgaaa aagaggccat
     3481 gggctggtat ctggacgggc gcgtggccgc ggtgctgggc acgcacacgc acgtgcagac
     3541 cgccgacgaa cgaatcctcc ccggcggcac cgcctacatc acggacgccg gcatgaccgg
     3601 cccgcgcgac agcgtcatcg gcggaaagat cgagatcgcg ctgaaacgct tcctcacggg
     3661 catccccctg cgcgtggacg tggccgacgg cgacgcccag ttcaacggct gcgttttgga
     3721 cctggacgag aagacgggga aagcgcgctc catcgaacgt ctgttcctcc tgatggaacg
     3781 agccaaggcc gaaaagcccg cctgaaccgc ccatgccgga ctcccgactg aaaaaatatt
     3841 tggataaaaa acggcggacc gtcgacgccg cgctgcgccg ttgtctgccg aaacacgccg
     3901 cccttccgcc ggtggtccac gaggcgatgc gttactcggt ctttgcggga ggcaaacggc
     3961 ttcgaccgat cctgtgcctg gcggccgccg aactctgcgg cggcacggcc aagcgggtca
     4021 tgcccacggc ctgcgcgctg gagctcatcc acacctattc gctggtgcac gacgatctcc
     4081 cggccatgga cgacgacgat ttccggcggg ggctgcccac cagccacaaa aagttcggcg
     4141 aaggcaacgc cgtcctcgcg ggcgacgcgc tgttgacgtt cgggttccag ctgatggccg
     4201 aaaacgcccg gcgggggaga gccagacccg ccgcggtgtt ggacgtcgtc cggacggtgg
     4261 cggaggccgc gggatcccgg gggatggtcg gcggtcaggc ggcggacctg gccatgtccg
     4321 gcaacgggaa gaaccggaac ggcgaaaaaa cgccggacgc cctgcgcaaa aaactggatt
     4381 acattcaccg caacaagacg gccgcgctca tccgggccag cctccgggcc ggcgccctcc
     4441 tggcgggggc ctccccggtc cggttaaacg cgttggacgc ctacggccga tccatcgggc
     4501 tggctttcca gatcgtcgac gatatcctcg acatcgtggg ggacaaaaaa ctcctcggca
     4561 aacgcgggtc ggaccgggac aacgacaagc tgacctttcc cgcgctctac gggctggagg
     4621 cctcgcgccg aatggcgcgg cgggaggtcc ggcgggccaa aaacgcgctc tccctcttcg
     4681 gcccccgggc gagaatcttg aacgacctgg ccgattacat cgtcgaacgt caaaactgaa
     4741 attcaggaga atcgcatggc tgtcttggac atgatcgaga aacctcagga cctgcggaac
     4801 atccgccggg aactgctccc tgatttggcg aaagagatcc gccagaagat cgtcgacgtg
     4861 accgcacaga ccgggggaca cctggggtcc agcctcggca ccgtggaact catcatcgcg
     4921 ctgcactact gcttcgattc gcccaaagac aagttcgtgt gggacaccgg gcaccaggcc
     4981 tatgcccaca aactgctgac gggccgacgg gaccggttcc ataccctgcg ccaattcggc
     5041 ggggtctcgg gcttcccgct gcgcgccgag tcggaacacg accatttcgg cgtgggacac
     5101 gcctccacct ccatctccgc cgcgttggga tacgcctgcg cgcgggacat cctgaaacaa
     5161 gaccaccgcg tggtggccgt ggccaacgac ggttccctca ccggcggcat ggccttcgag
     5221 gccctgcaga acgccggcat cctcggcacc aacctgctgg tggtgctgaa cgacaaccag
     5281 atgttcatct cgcatcgggt gggagccttc ggcgcctttc tggcgaaact cctcaccctg
     5341 ggcagcgtga agcgcatgga gaagaaggtc gaacggttcc tcacgcgcat caatttctgg
     5401 ggggcctacc tccttcgcct ggcccggcgg atcaaggtgt tcctgttccc gggggtgttg
     5461 ttcgaggaaa tggggttcgg ctacatcggc ccggtggacg gacacaacct caaccagttg
     5521 atcgacgtca tcaccgccgt gaaacagctc accggcccga gggttctcca cgtggtcacc
     5581 aaaaaaggcc gcggctacga accggccgag caggaccccg tccgctacca cggcgtttcc
     5641 cggttcgacc cggaaacggg ggacatggtc aaactggaag ccggaccgcc gtcctacacc
     5701 aaaattttcg gccaggcgct ggtgaaactg gccaaggacg atcccaaagt cgtcgccatc
     5761 acggcggcca tgccggacgg caccgggctc gacctcttcc gcaaggagtt ccccgaccgc
     5821 ttcttcgacg tggggttggg agaacagcac gcggtcacgt tcgcggccgg gctggcctgc
     5881 ggcggg
//
LOCUS       DAOTFD010000166         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9706_length_5883_cov_4.472032, whole genome shotgun sequence.
ACCESSION   DAOTFD010000166 DAOTFD010000000
VERSION     DAOTFD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_5883_cov_4.472032"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1242)
                     /gene="recF"
                     /locus_tag="P5079_08905"
     CDS             complement(106..1242)
                     /gene="recF"
                     /locus_tag="P5079_08905"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRY30138.1"
                     /translation="MFLRSLSLRQFRNYGRLDVEFDPRLNVFIGPNGSGKSNLMEAIA
                     VLATGQSHRGAELRHLLQWEKEGFAVKGAFEGEDEVLLEVRQKSGRPRQTLLNNAVQK
                     RLKDWVGRVPVVTFSPDDLSLVKGEPGGRRRLLNAVLCQVDGDYMETLQRYNKTLAER
                     NAALRQVQDHERPPASLEPWDASLLKLGASLSLARRIFLEKFQEYARRRHAALSSGRE
                     TVRLSYRPSFLLPSGEAEEVVAANRRRLQDLREGEIALGSTLIGPHRDEVEFFLGENL
                     ARAYASQGQMRTLALAVKLAEQDFLTARLKKQPLCLFDDMLSELDPDRRENLVEALTS
                     GTQCMVTMAAAAEWPRLMESLDGRNSCLFEVRDGKLTASAKRSF"
     gene            complement(1319..2428)
                     /gene="dnaN"
                     /locus_tag="P5079_08910"
     CDS             complement(1319..2428)
                     /gene="dnaN"
                     /locus_tag="P5079_08910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY30139.1"
                     /translation="MQVHCLKEELLRGVQLVQNAISPRSTLPVLSNILMESTGEGLRM
                     SSTDLEVGIRCFVKADVKTGGATTVPARTLGEFLRTLDDGQDLDIKVDESQKMEIRSG
                     KDRCVLMGLPKDDYPVLPEFSQERAFSVNQNTLREMIRKTSFAVSTDETRYVLNGVDF
                     IVDKGKITLVSTDGRRLAYVQRDCGDKKAVANAIIPTKAVNELARILTGDEKDGVVQI
                     GFTENQVTFNYKGTVVLSRLIEGHFPNFEQVIPKSHDSQIKFNTRKLLLATQRAAVGT
                     LEKGGSVRYSLTKGKLQISASAQGRVEVESELEAAYTGAPFAIAFNPAYLVDMFRVLE
                     TDDVLLELTTPLNPAVIRPVGDEHYKYVVMPMQLT"
     gene            complement(2721..4103)
                     /gene="dnaA"
                     /locus_tag="P5079_08915"
     CDS             complement(2721..4103)
                     /gene="dnaA"
                     /locus_tag="P5079_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY30140.1"
                     /translation="MIVSPTAAELWEDIANSLKTSLKQESFDLWIKPLSPLRWENGLL
                     TLQVPNHFYSDWVKKNCQDAIESALRQASGSEAAIAFEFAQEIETILKQEVARVDPVP
                     MPRAENAFTEEQFNPKYTFDTFVVGPSNRFAQAAAEAVAKDPGRAYNPLFIYGGVGLG
                     KTHILHAIGHHIRSSRPNARVLYISSEKFINEFIDAIRFERMKDFRAKYRSLDCLLID
                     DIQSLVGKDNSQEEFFYTFNSLYETQKQIVVCSDRSPKETRVEDRLRSRFEWGVIADI
                     QAPDLETRIAILRKKASAERIFVPDDVILYIASQIRSNIRELEGSLIRIVAFSSLTGT
                     PLTVDSARETLKDIVSAAEAVRPITIELIQEVVAKHFNLDIKDMRSKRRTDAIAFPRQ
                     IAMYLARTLTECSTTEIGENFGGKDHTTVMHACNKIKSKMSSDPYFLALVNKITQHIK
                     NSVTLDQLKS"
     gene            complement(4409..>5883)
                     /gene="uvrA"
                     /locus_tag="P5079_08920"
     CDS             complement(4409..>5883)
                     /gene="uvrA"
                     /locus_tag="P5079_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY30141.1"
                     /translation="KQVARQVLKEILSRLTFLSDVGLEYVTLDRASGTLAGGEAQRIH
                     LATQIGSGLVGVLYVLDEPTIGLHPRDNRRLLSTLEHLRNLGNTLVVVEHDEETIRAA
                     DWVVDLGPGAGVHGGEVVMSGPVPALLKSPDSLTGAYLRGEKQIPRPAQRRIPGKNWL
                     EIHGASQFNLKEIDARFPLGLFIGVTGVSGSGKSTLVQEVLYKAMARKLYNAKEEPGA
                     HKALRGMEHVDKVIVVDQTPIGRTPRSNPATYAGAFSDIRDLFAQLPEARRRGYKPGR
                     FSFNVKGGRCENCEGDGTLKISMQFLPDVYVKCEVCGGKRFNEDTLQIKYKGKSIADV
                     LAQSAEEAAVFFENVPPVARVLKTLVDVGMGYAAIGQPATTLSGGEAQRVKLATELCR
                     RATGRTLYILDEPTTGLHFADIEKLLGVLHRLVDNGNTVLIIEHNLDVIKTADWLIDL
                     GPEGGAGGGRIVAEGSPEKVAQVAASHTGHYLKKLLAAEK"
BASE COUNT         1069 a         1622 c         1939 g         1253 t
ORIGIN      
        1 cggcgtagtt ctcggggtta tagcccagct tggagagcca gtcggcaaaa agattcttcg
       61 ccggaacagg tcctgggggc aggttatggc cgtgcggttt tctcttcaaa aggacctctt
      121 ggccgaggcg gtcagttttc cgtcgcggac ctcgaacagg caggaattcc ggccgtcgag
      181 agactccatc aggcggggcc actcggcggc cgccgccatg gtgaccatgc actgcgttcc
      241 ggaggtcaac gcctccacca ggttctcccg gcggtccgga tccagttcgg aaagcatgtc
      301 gtcgaagagg cagagcggct gtttcttgag ccgcgcggtc aagaaatcct gttcggccag
      361 tttcaccgcc agcgccagcg tccgcatctg tccctgggag gcgtaggccc gggcgaggtt
      421 ctctcccaaa aaaaactcga cttcgtcccg gtgcggtccg ataagggtgg acccaagggc
      481 gatttccccc tcgcgcaggt cctgaagacg gcggcggttg gcggcaacga cctcttcggc
      541 ttctccggac ggcaggagga acgacggccg ataggacaaa cgcaccgtct cgcggccgga
      601 agagagggcc gcatgccggc gacgggcgta ttcctggaac ttctccaaaa aaatccgacg
      661 ggccaggctg agcgaagccc ccaatttcag gagagaggcg tcccacggtt ccaacgaggc
      721 cgggggcctc tcatggtcct gcacctgacg caaggcggcg ttccgttccg ccagcgtctt
      781 gttgtagcgc tggagggtct ccatgtaatc gccgtccacc tggcagagga cggcgttgag
      841 cagccggcgg cggccgccgg gttcgccctt gacgagggac aggtcgtccg gtgagaaggt
      901 gaccaccggg acgcggccga cccagtcttt gagacgtttt tggacggcgt tgttgagcaa
      961 cgtctggcgc ggccggccgg acttctggcg cacctccaag aggacttcgt cctctccttc
     1021 gaaagcgcct ttgacggcga atccttcttt ttcccattgg agaagatggc gcagttcggc
     1081 gccgcggtgg ctctgaccgg tggccagaac cgcgatggct tccatgaggt tggatttccc
     1141 ggaaccgttg ggtccgatga agacgttgag gcgcgggtcg aactccacgt cgagccggcc
     1201 gtagttgcgg aactgtctga gggaaagaga tctaaggaac acgagtgaca ggtgtcaggc
     1261 cgcgggcggc ggacgacggg aaaaccgctc ccggaccttc gacgccgaac acccgtcgtt
     1321 aggtcagctg catgggcatg acgacgtact tgtaatgctc gtcgcccacg ggccggatca
     1381 ccgccggatt gagcggggtg gtgagttcaa gaaggacgtc atccgtttcc agcacgcgga
     1441 acatgtccac gaggtaggcc gggttgaacg cgatggcgaa gggagcgccc gtgtaagcgg
     1501 cttccagttc ggattccacc tccacgcggc cctgggcgga ggcggatatc tgcagtttgc
     1561 ccttggtgag gctgtatcgg acggagccgc ccttttccag cgtgccgacg gcggcccgtt
     1621 gggtggccag aaggagttta cgggtgttga actttatctg ggagtcgtgc gatttgggga
     1681 tcacctgttc gaagttgggg aaatgcccct cgatgagacg ggacaggacg acggttccct
     1741 tgtagttgaa cgtcacctgg ttctcggtga acccgatctg gacgacgccg tctttttcgt
     1801 cgccggtgag gatgcgggcc agttcgttga ccgccttggt gggaatgatg gcgttggcca
     1861 cggctttttt gtcgccgcag tcgcgctgga cgtaagccag gcggcggccg tcggtggaaa
     1921 cgagggtgat cttgcctttg tccacgatga aatccactcc gttgagcaca tagcgggttt
     1981 cgtcggtgga gacggcgaag gaggtcttgc gaatcatctc acgcagggtg ttctggttga
     2041 cggagaaggc ccgttcctgg ctgaactccg gcaggacggg ataatcgtcc ttgggaaggc
     2101 ccatgagcac gcagcggtct ttccccgaac ggatctccat tttctgactc tcgtccacct
     2161 tgatgtccag gtcctggccg tcgtccaggg tgcggaggaa ttctcccagg gtgcgggccg
     2221 gaacggtggt ggcgccgccg gtcttgacgt ccgctttcac gaagcaacgg atccccactt
     2281 ccaaatcggt ggacgacatg cgcagtcctt cgccggtgga ttccatcagg atgttcgaca
     2341 acacgggcag ggtgctccgt ggcgaaatgg cattttggac cagttgaact cctcggagga
     2401 gttcttcttt caaacaatgg acttgcatgg atgtcttggc ctcccttgtt ctttgctcgt
     2461 caatggagcg ttataggatg aaatccccga tacacacagc gctacttctt taaaagagta
     2521 tttaaacatt agccgtcgta ttagtaggga ccgggacggc ggggataaac cccgtgaaac
     2581 ggccgaaagc tttttgaatc aacgactttt gaaattttgc gtggtcgaac aacctgtgga
     2641 aaatcggccg gtgtttttca cactgttcgc gatgtaacgg gatttcccca acctcggcga
     2701 cttatgcaga agttattcag ctacgacttc agctggtcca acgtgacgct gttcttgatg
     2761 tgctgcgtga ttttgttgac cagcgccagg aagtacgggt ccgaagacat cttggacttg
     2821 atcttgttgc aggcgtgcat gaccgtggtg tggtctttgc cgccgaaatt ctcgccgatc
     2881 tccgtggtgg aacattccgt cagcgtgcgg gccaggtaca tggcgatctg ccgcgggaac
     2941 gcgatggcgt cggtgcggcg cttggagcgc atgtccttga tgtccagatt gaaatgtttg
     3001 gccaccacct cctggatcag ctcaatggtg atgggccgca cggcttccgc cgcggagacg
     3061 atgtccttta aggtctcccg cgccgaatcc acggtgagcg gagtgccggt gagggaggag
     3121 aaggccacga tacggatgag gctgccttcc agttcgcgga tgttggaccg gatctggctg
     3181 gcgatataga ggatgacgtc gtccgggacg aagatccgtt cggccgaggc ctttttgcgc
     3241 aggatggcga tgcgggtctc caggtcgggg gcctggatgt cggcgatgac gccccattcg
     3301 aatcgggacc gcagccggtc ttccacccgc gtttctttgg gagagcggtc cgaacagacc
     3361 acgatctgct tctgggtctc ataaagggag ttaaaggtat agaagaactc ttcttgggag
     3421 ttgtctttcc ccaccaggga ctggatgtcg tcgatgagga ggcagtccag gctgcggtat
     3481 ttggcgcgga aatctttcat gcgttcaaaa cggatggcgt cgatgaattc gttgatgaat
     3541 ttttcggagg agatatagag gacgcgggcg ttgggtcggg aggaacggat gtggtgcccg
     3601 atggcgtgga ggatgtgggt ctttccgagc cccacgccgc cgtagatgaa gagggggttg
     3661 taggcccggc cggggtcttt ggccaccgcc tcggcggcgg cctgggcgaa gcggttggac
     3721 ggccccacca cgaaggtgtc gaaggtgtat ttggggttga actgttcttc ggtaaaggcg
     3781 ttctcggccc ggggcatggg gaccggatcg acccgggcga cctcctgttt caggatggtc
     3841 tcgatctcct gggcgaactc aaaagcgatg gcggcctcgc tgccggaagc ctggcggagg
     3901 gcggattcaa tggcgtcctg gcagtttttc ttgacccaat cggaataaaa gtggttcggc
     3961 acctgaaggg tcaggagtcc gttctcccag cggagggggc tgagaggttt gatccaaagg
     4021 tcgaaagact cctgtttaag ggaggtcttc agggaattag cgatatcttc ccagagctcg
     4081 gcggcggtcg gcgaaacgat cactttttgg gggctccccg atcaagttac ccacagactt
     4141 gcgcacaatt gtggataact cttgtgaatt atcttgatat gttttttact accatcaagt
     4201 gctatctttt agtgctgagt cgcgcaaaaa aaacccttac caggcgacat tcatcggctt
     4261 ttgcatggaa agggctggcg catctcaaat gtgtggatat cctaattcat caacgaaaac
     4321 ttgtcaagag tttttgaaaa aatttacaag aaacgatttt ccgtcggtgg atgttcgtcc
     4381 catttccaaa aaaaacagag tcccgtgatc atttttccgc ggcaagaagt tttttgaggt
     4441 aatggccggt gtgggaggcg gcgacctggg cgactttttc gggagagccc tcggccacga
     4501 tgcgtcctcc gccggcgccg ccttccgggc ccaggtcgat gagccagtcg gcggtcttga
     4561 tgacgtccag gttgtgttcg atgatgagca cggtgttgcc gttgtccacc aggcggtgca
     4621 ggacgccgag gagtttctcg atgtcggcga agtggaggcc ggtggtgggc tcgtccagga
     4681 tgtaaagggt gcggccggtg gcgcggcggc agagttcggt ggccagtttc acccgttggg
     4741 cctcgccgcc ggagagggtg gtggcgggct gaccgatggc ggcgtagccc atgcccacgt
     4801 ccaccagggt tttcaggacg cgcgcgacgg gcggcacgtt ctcgaagaat accgccgcct
     4861 cctccgccga ctgagcgagc acgtcggcga tggatttccc cttgtatttg atctgaaggg
     4921 tgtcctcgtt gaaccgcttg ccgccgcaca cctcgcattt cacgtacacg tccggaagga
     4981 actgcatgga gatcttcagg gtgccgtcgc cctcgcagtt ttcgcagcgg ccgcccttga
     5041 cgttgaagga gaagcggccg ggtttgtatc cccgccgccg cgcttcaggc agttgggcga
     5101 aaaggtcgcg gatgtcgctg aaggctccgg cgtaggtggc ggggttggag cggggcgtcc
     5161 ggccgatggg cgtctggtcc accacgatga ccttgtccac gtgttccatc ccccggagcg
     5221 ccttgtgggc gccgggctcc tccttggcgt tgtagagttt ccgggccatc gccttataaa
     5281 gcacctcctg cacgagggtg gatttcccgg agccggacac cccggtgacg ccgatgaaga
     5341 ggcccagcgg aaagcgcgcg tcgatctctt tcaggttgaa ctgggacgcc ccatggatct
     5401 ccagccagtt ttttcccggg atccggcgtt gagccggccg gggaatttgt ttttctccgc
     5461 gcagatacgc gccggtgagg gaatcaggag acttcaacag cgccggcacc ggcccggaca
     5521 tcaccacctc gccgccgtgc acgccggccc cgggacccag gtccaccacc caatccgcgg
     5581 cccggatcgt ctcctcgtcg tgttccacca ccaccagggt gttccccagg ttgcggaggt
     5641 gttccagggt ggagagcagg cgccggttgt cgcgcgggtg gaggccgatg gtgggctcgt
     5701 ccaggacgta gagcacgccc accaagccgg acccgatctg ggtggccagg tggatgcgct
     5761 gagcttctcc gccggccagg gtgccggagg cgcggtccag cgtgacgtat tccaggccga
     5821 cgtccgagag gaacgtgagc cgcgaaagga tctctttcag gacctgccgg gccacctgtt
     5881 tgt
//
LOCUS       DAOTFD010000167         5808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9878_length_5808_cov_3.840431, whole genome shotgun sequence.
ACCESSION   DAOTFD010000167 DAOTFD010000000
VERSION     DAOTFD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9878_length_5808_cov_3.840431"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="P5079_08925"
     CDS             <1..515
                     /locus_tag="P5079_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY30142.1"
                     /translation="ASFARNKGEVMCLNCDGGMMMNLQELQTIAHHKLPIKLFIFNND
                     GYLMIKHTQKAIVGGRYSGTDKKSGVSCPDYSALAKAFNMPAFQIRTWDDFDRIIPQV
                     QSATGPIICEVFMHPEQLFVPKLGLSIQKDGSLISPPLEDLSPFLPREELRRNMITPL
                     HPKSEKIESQ"
     gene            complement(560..1633)
                     /locus_tag="P5079_08930"
     CDS             complement(560..1633)
                     /locus_tag="P5079_08930"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY30143.1"
                     /translation="MNERADKLVREDCRRVLDGREKQLAALKGDTLFITGGTGFMGTW
                     LAEMLACLNDDFSFKTKVFLMARNTDKFQGVCPHLAGRKDMTLVKSDIRRLSELPKQT
                     HWVIHAAVTPDNRFHSSRPVETMSTVSEGIAAVIRAADPCSDFKKLLNISSGLVYGTQ
                     PWETERIPESHVGGPVMGNVASVYAEAKRYAEIYCAAARSQQKLPVVTARPFAFVGPY
                     QSLNTPWAINNFIHDALNAKIIRVLGDGKTVRSYLYGADMSFWLLRILVDGVSGQAYN
                     VGSPDGISLEQLAGLIAEQFDPQPEIRIQASVPAVTQRTRFVPDVTLAAEGLKLSVKT
                     PLKAAIKQTIEWNKLSRSTAVAV"
     gene            complement(1630..2646)
                     /gene="dmpG"
                     /locus_tag="P5079_08935"
     CDS             complement(1630..2646)
                     /gene="dmpG"
                     /locus_tag="P5079_08935"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HRY30144.1"
                     /translation="MNKILISDPTLRDGNHAANHQISVQQIAAYCDGADAAGVPIVEV
                     GHGNGIGASSLQLGESPIPDFDILRTAREHLPHSKLGIHVIPGFATIEKDLKPAIRAG
                     VDVVRVASHCTEADITERHIGFAREAGKTVFGVLMMSHMASKELLAEEAKKMESYGAE
                     GFIFMDSSGHFLPDDVTERVKTLLDVLEIPVGFHAHNNLGMAIANSIAAAKAGATMLD
                     GTARGFGAGSGNAQLEVLVAVLERLGFKTGIDLYKLLDLSDLADRELLKEIPTIRSVS
                     VVSGLAGVFSGFTKHVYRIAKQYEVDPRDVFFELGKRGVVAGQEDLIIEVASDLARKA
                     KASS"
     gene            complement(2643..3539)
                     /locus_tag="P5079_08940"
     CDS             complement(2643..3539)
                     /locus_tag="P5079_08940"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HRY30145.1"
                     /translation="MKKLKVAIIGSGNIGTDLLIKVMRSPHLECGAFIGRNLSSPGMA
                     KATAMGMNVSSESISYIESNPDCCELVFDATSAVDHQRHAPILRKLGKISIDLTPAQV
                     GQMCVPAVNMRECLSEDNVNMVTCGGQASIPVAYALAQSHSGIDYIEVVSSIASRSAG
                     PATRRNLDEYIHTTENGLRKFSGCRRTKAILNLNPAQPCVNMQTTVMAKVSNPKLDEF
                     TKIFQKIAQTIREYVPGYETVVPPTIENNRIFTMVRVRGLGDYLPAYAGNLDIINCAA
                     IAMAEEYAKNRRPALTEAGAPK"
     gene            3636..4415
                     /gene="rfbF"
                     /locus_tag="P5079_08945"
     CDS             3636..4415
                     /gene="rfbF"
                     /locus_tag="P5079_08945"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HRY30146.1"
                     /translation="MKVVLLAGGQGTRLREETEYRPKPMVNIGRWPVLWHIMKIYSHF
                     GFNEFVICLGYKGEMIKEYFLNYRMMANDFTIHLGKHPVAFHNGNNDLNWKVTLVDTG
                     PNAMTGARLKRIQPHIKEDTFMLTYGDGVADIDVRALVKFHRKHGKLATVTGVTPPSR
                     FGELVVRGDRVVEFSEKPQTRAGLISGGFFVLNTKVFDYLTADDDCIFEQKPLEELTK
                     NGQLMVRPHKGFWHCMDTLRDVHYLNSLWDKGDAPWKVWRW"
     gene            4409..5485
                     /gene="rfbG"
                     /locus_tag="P5079_08950"
     CDS             4409..5485
                     /gene="rfbG"
                     /locus_tag="P5079_08950"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRY30147.1"
                     /translation="MVNTQFWHGKRVFITGHTGFKGSWLCLWLHSLGAKVTGYSLKPP
                     TRPSLFELARVGKLVKSVHGDVRDGTHLKKVMSAARPEIAFHMAAQPLVRESYKNPIE
                     TYETNVLGTVNFLEAVRHATGLHAVVNVTTDKCYENREWVWGYREDEPMGGHDPYSNS
                     KACSELVTASFRSSFFNPKDYRRHQVALASARAGNVIGGGDWAEDRLIPDCIRAILKG
                     EKVLIRNPNSIRPWQHVLEPLNGYLTLAQKLHTDGPHYAEGWNFGPDESDAKPVLWIV
                     KRLCALWNNDAGYRIDRRKHPHEANYLKLDCSKAKTRLGWQPRWNLEYAIDHIVEWTE
                     SYVAQTDLQKVCLAQIKDYETTSS"
     gene            5518..>5808
                     /locus_tag="P5079_08955"
     CDS             5518..>5808
                     /locus_tag="P5079_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30148.1"
                     /translation="MDTALAHAKMEQILSKPHSSQPLRTFFSYAILTFLTVTLAAYFL
                     QLCRADISTSFYYCYGGDALFTQSLAKGIVENGWYLINPRLGAPQGQQLYDYP"
BASE COUNT         1359 a         1630 c         1469 g         1350 t
ORIGIN      
        1 gcgcctcctt tgcgcggaac aagggggaag tcatgtgctt gaattgcgac ggtggcatga
       61 tgatgaatct gcaggagctc cagaccatcg cgcatcacaa gcttcccatt aaactgttta
      121 ttttcaacaa cgatggctac ttgatgatca aacacacgca aaaagccatc gtcggcggac
      181 gttattccgg tacggataag aaatcggggg tcagttgtcc tgattattcc gccttggcca
      241 aggcattcaa tatgccggcg tttcaaatcc gcacgtggga tgattttgac aggattattc
      301 ctcaagttca gtccgccaca ggtcctatca tttgtgaggt attcatgcac cccgaacaac
      361 tttttgtgcc aaaactgggg ctttccatcc agaaagacgg ctccctgatc tcgccgccgc
      421 tggaagattt gtcacccttc ctcccccgag aagagctgcg gcgcaatatg attacaccgc
      481 tccatcccaa atcggagaag atagagtctc aatagctatt tttgagtggg ttttttagcg
      541 gaaagagtcg cggaaaatat tacaccgcaa cagccgtcga gcgagacaat ttattccact
      601 caatggtctg tttaattgcg gcttttaagg gagtcttgac ggaaagcttt aatccctcgg
      661 cggccagagt aacatccggc acaaacctcg tacgctgtgt caccgccgga actgaggcct
      721 gtatccgtat ttccggctga ggatcaaact gctcggcaat caagccggcc aactgctcta
      781 aactgattcc atcagggctg cccacgttgt aagcctgccc cgaaacaccg tccaccagta
      841 tccgcaacaa ccaaaaactc atgtccgctc catacaaata gcttcgcacc gtcttgccgt
      901 ctcccagcac ccggatgatt ttggcgttca aggcgtcgtg aatgaaatta ttgatggccc
      961 atggggtatt gagggactga tacggcccca caaacgcaaa tggccgagcc gtcaccaccg
     1021 gcagtttctg ctggctccgc gccgccgcgc aatagatctc ggcatagcgc ttcgcctcag
     1081 catacacgga ggcgacgttg cccatcaccg gccccccgac gtgtgattcg ggaatccgct
     1141 ccgtttccca gggctgtgtc ccataaacca aaccggaact gatgttgagg agttttttga
     1201 agtcggagca cgggtccgct gcgcggataa cggcggcaat cccttcggaa acggtgctca
     1261 tggtctccac ggggcgggag gaatgaaacc ggttgtccgg cgtgacggcg gcgtggatca
     1321 cccaatgggt ctgcttggga agttcgctga gacggcggat gtcggatttg acgagcgtca
     1381 tgtccttccg tccggccaga tgcgggcaga ccccctgaaa cttgtccgta ttgcgggcca
     1441 tgagaaaaac tttcgtcttg aaagaaaaat cgtcattcag gcaggccagc atttccgcca
     1501 gccacgttcc cataaaaccg gtgccgccgg tgatgaagag ggtgtctcct ttcaatgcgg
     1561 ccagctgttt ctctcggccg tcgaggacac gcctgcagtc ctcgcgaacc agtttgtctg
     1621 cgcgttcgtt catgacgatg cctttgcctt tctcgccagg tcgctggcaa cttcgatgat
     1681 caagtcttcc tgcccggcca ccacgcctcg ttttcccaac tcaaaaaaaa cgtcgcgggg
     1741 gtccacctcg tattgttttg caattcgata gacgtgcttg gtgaaaccag agaagacgcc
     1801 agccaaaccg ctcaccacac ttacggaccg gatagtcggg atttctttca acaattctct
     1861 gtccgccaga tcggacagat ccagtaattt ataaagatca atccccgttt tgaaaccgag
     1921 ccgttccaaa acagccacca acacctcaag ttgagcattg ccagaaccgg ccccgaatcc
     1981 ccgggcagtg ccatccaaca tggtggcccc cgccttggcg gcggcgatgg aattggcaat
     2041 ggccatcccc aagttgttgt gggcatggaa ccccacgggg atttcaagaa catcaagaag
     2101 cgttttgact cgttctgtca catcgtcggg caaaaaatgc ccggaagaat ccatgaatat
     2161 aaacccttcg gccccatagg actccatttt tttggcttcc tctgcgagga gttctttgga
     2221 ggccatgtgg gacatcatca atactccgaa aactgttttg ccggcctccc gggcaaaacc
     2281 gatgtgccgt tccgtgatat cggcttccgt gcagtgggac gccaccctta ccacatctac
     2341 tccggcccga atggccggct tgaggtcttt ttcaatggtg gcaaaccccg gaatcacatg
     2401 aatgcccaat ttcgagtggg ggagatgttc ccgggccgtc cgcaggatat cgaaatcggg
     2461 gataggtgat tctcccaact gcagggacga cgccccaatg ccgttgccat gtccgacctc
     2521 gacgatgggg acacccgcag cgtcggcccc atcacaatag gcggcaattt gctgaactga
     2581 tatttggtgg ttcgccgcgt ggttgccgtc gcgcaaagtg ggatcgctga tcaatatttt
     2641 gttcattttg gagcgcctgc ctctgtcagc gccggtcgcc ggtttttggc gtattcttcc
     2701 gccatggcaa tggccgcgca attgatgatg tctaagttcc ccgcgtaagc cgggagataa
     2761 tctcccaacc cgcgaaccct caccatggtg aaaatccgat tattttcgat ggtggggggg
     2821 accacggttt cgtatccggg aacatattcc cggatagtct gggcaatttt ttgaaatatc
     2881 ttcgtgaatt catccagttt agggttggac accttggcca ttaccgtggt ctgcatgttg
     2941 acacacggct gggcgggatt gaggttcaaa atggcttttg tccggcggca gccggaaaat
     3001 ttccgaagcc cgttttccgt tgtatggatg tattcatcca aattgcgccg ggtggccggc
     3061 ccggcactcc gagaggcgat gctggagacg acttcgatat agtcaattcc cgagtgactc
     3121 tgcgccaacg cgtaggccac gggaatggat gcctgcccgc cacaggtgac catgtttaca
     3181 ttatcttcgc taaggcattc ccgcatgttg acggcaggca cgcacatttg tccgacctgt
     3241 gctggcgtca aatcaatgga gatcttcccc agcttcctca aaatcggggc gtggcgctga
     3301 tggtccaccg cggacgtagc gtcaaaaacc agttcacagc agtccggatt actctcgatg
     3361 taggagatgc tctcgctgga aacgttcatc cccatggccg tagccttggc catgcctggg
     3421 gatgaaagat tgcggccgat gaacgcccca cactccagat gaggggaccg catcacttta
     3481 atcagcaggt ccgtcccgat gttcccgctg ccgatgatgg caaccttcag cttcttcatt
     3541 gctaaaaatt atagcaaacc gccccctttg ccgcacggca aatgttggtt ttatggtata
     3601 atttgaccaa ttacccctac aaaaggagag tccatttgaa agttgtgtta cttgccggcg
     3661 gacaaggcac ccgccttcga gaagaaaccg aataccgccc caaaccgatg gtgaatatcg
     3721 gacgatggcc cgttctttgg cacatcatga agatttacag ccatttcggt ttcaatgaat
     3781 tcgtcatctg cctgggatac aagggggaaa tgatcaagga atatttcctc aactaccgca
     3841 tgatggccaa cgacttcacc atccacctcg gcaaacatcc cgtcgcgttt cataacggga
     3901 acaatgattt aaattggaaa gtgaccctcg tggatacggg ccccaacgcc atgaccggcg
     3961 cccgcctgaa acggatacaa cctcacatca aagaggacac cttcatgctt acctacgggg
     4021 acggcgtcgc cgatattgac gtgcgtgcct tggtaaaatt tcaccgcaaa cacggaaaac
     4081 ttgccaccgt gaccggcgtc actccgccgt cgcgcttcgg tgaattggtg gtgaggggcg
     4141 accgggtcgt tgaattcagc gaaaagcccc aaacccgggc tggactgatc agcggcggct
     4201 tctttgtgct gaacacaaaa gtgtttgatt acctcaccgc cgatgatgac tgcattttcg
     4261 agcagaaacc cctcgaggaa ctgacaaaga acggccaact gatggtccgt ccgcacaaag
     4321 ggttctggca ttgcatggac actttgcgtg acgtgcatta cctcaactct ttgtgggaca
     4381 aaggcgacgc gccctggaag gtgtggagat ggtgaatacc caattttggc atggcaaacg
     4441 ggttttcatc accggtcata caggattcaa ggggtcttgg ctgtgcctct ggctccattc
     4501 cctgggagcc aaagtcaccg gttactcttt aaaacctccc acccgaccca gtctcttcga
     4561 gttggcaagg gttgggaaat tggtcaaatc cgtccacggt gacgtgcgtg acggcacgca
     4621 cctgaaaaaa gtcatgtccg cggcacggcc ggagatcgct ttccatatgg ccgcccaacc
     4681 cctcgttcgt gaatcctaca aaaaccctat cgaaacctat gagaccaacg ttctgggcac
     4741 tgttaatttt ctggaagccg tgcgccacgc aaccggactt cacgccgttg tcaatgtcac
     4801 caccgacaaa tgttatgaaa accgggagtg ggtctgggga taccgcgaag acgaaccgat
     4861 gggcgggcac gacccctatt ccaacagcaa ggcttgttcc gaactggtga ctgcctcttt
     4921 ccgcagttct tttttcaatc ccaaagatta tcggcggcat caggtggcgc tcgcatcggc
     4981 acgagcaggg aacgtcatcg gtggtggcga ttgggcggaa gaccgactga ttcccgactg
     5041 catccgcgcc attttgaagg gggaaaaagt cctgatacgg aatccaaact ccatcaggcc
     5101 gtggcaacat gttttggaac ctttgaacgg ctatctaacc ttagcgcaga aactacatac
     5161 agacgggcct cattacgcgg aaggctggaa tttcggtccc gacgagagcg acgcaaaacc
     5221 cgtgctttgg atcgtcaaac ggttatgcgc tctctggaac aacgacgccg gctatcgaat
     5281 cgaccgccgc aaacacccgc atgaggccaa ctatttaaaa ctggactgtt ccaaagccaa
     5341 aacccgcctc ggctggcagc ctcgctggaa tttggaatac gccattgacc atatcgtgga
     5401 atggacagaa tcttatgtcg cacaaactga tttacagaaa gtttgtttag ctcaaattaa
     5461 agattacgaa acaacttctt cctgatccca ttgccacttt ctttttccat aaatgaaatg
     5521 gacacagctt tggcgcacgc taaaatggaa caaattttat ctaaaccgca ctcatcccaa
     5581 ccactgcgca catttttttc ttatgccata ttgacctttc tcaccgtcac tcttgcggcc
     5641 tatttccttc aactctgccg cgctgatatt tcaacgtctt tctattattg ctatggagga
     5701 gacgcgttat ttacgcaatc gcttgcaaaa ggcatcgttg aaaacggctg gtacctgatc
     5761 aatccccgcc tcggcgctcc tcaagggcag caactttatg actaccct
//
LOCUS       DAOTFD010000168         5786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_9927_length_5786_cov_4.582621, whole genome shotgun sequence.
ACCESSION   DAOTFD010000168 DAOTFD010000000
VERSION     DAOTFD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_5786_cov_4.582621"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="P5079_08960"
     CDS             <1..1621
                     /locus_tag="P5079_08960"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRY30149.1"
                     /translation="LEYAEWAGRQEADVVVFPELSLPGYPPEDLVMRRHFQDDTRRAL
                     TELAKKMPAGPPALVGFTETAGTDLHNAAAVLFGGKIRAVYRKQRLPNYGVFDEKRYF
                     APGKELIFLKIRGVCAGITLCEDVWADDGPAPALAKQGAQVILNLSASPYHAGKQSLR
                     RRTIGEVVKKCGRPLVYCNMVGGQDELVFDGGSMAMDAHGRIVADAPQFEECLLTADI
                     PVPPATRFPKNIVPLSPAPAGTKIPIEPPPSAAPLSELDEIYSALVLGTRDYVKKNGF
                     KKAVLGLSGGIDSSLVACVAVDALGKENVIGVTMPSRFNSPETRSDAEILSRNLGIGF
                     HSVPIETVTEEFLKVLRPVFGDRPRDLAEENLQARVRGTLLMALSNKFGWMVLTTGNK
                     SEVSVGYSTLYGDTAGGFAVIKDIPKTLVYRLARRRNEKAGRQLIPQSVFDRPPTAEL
                     RPNQTDQDSLPPYELLDNIIRLYVEEDKGLGEIVKSGVGSEIAAKVLRLVDASEYKRR
                     QAPPGVKITPKAFGRDRRMPITNRYRPGQPN"
     gene            1640..2431
                     /locus_tag="P5079_08965"
     CDS             1640..2431
                     /locus_tag="P5079_08965"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY30150.1"
                     /translation="MDIKNKVILITGASSGIGWATAEALAREGARLALAARRQDRLDA
                     LAQTLHERHGTETFVVRTDVKRENEVRYMITETVKRFGRLDVLINNAGVLFMGQFLEM
                     PLVDMHAIMDTNYWSIVHAVKFAAPPMSKQGGGHIINIASGVSRRGLPFMAAYSASKY
                     ALAGLTEAVRLELAAQNIKFTTVYPGGVETEMPKSVDRKKLPPTYPDQRRWRISAERV
                     ARAVVKAVRKQPVEIFVPWWVGIGPWINLFSPALADRLIRRAEKI"
     gene            2438..3511
                     /gene="ychF"
                     /locus_tag="P5079_08970"
     CDS             2438..3511
                     /gene="ychF"
                     /locus_tag="P5079_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRY30151.1"
                     /translation="MDIGIVGLPNVGKSTLFNALTGAAAAASNFPFTTIDPNVGIVPL
                     PDERLNVLGKMFASEKVTPAGVRFVDIAGLVKGASQGEGLGNQFLSHIRAVDAVAHVV
                     RAFHDENVVNVLGKLDPLESADIIETELLLADLQQAQKLLDKLQGPARSGDKKAKERL
                     AQMEQIIKGFQEGKSARMQNLPEDVNREYEFLTGKPLLYVANVDERGGEPTLLAPLTE
                     RAKRENAKVVVLCAKIEAEIVQLAEEERASFYESAGIKQSGLAELAHAGKDLLDLICF
                     FTAGPKESRAWIIPKGTKAVKAAGKIHSDIERGFIRAEMYKYDDLVKLGSYKAVQEKG
                     LVSMEGKDYEMKDGDVVYFRFNV"
     gene            3531..5213
                     /locus_tag="P5079_08975"
     CDS             3531..5213
                     /locus_tag="P5079_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRY30152.1"
                     /translation="MSLALIATVCTLLFVAAYFLYGRLAARLFKLDPQTPTPAKVYED
                     GVDFVPAKASLLLGQHFSAIAAAGPIVGPILAGIWFGWLPALLWIVLGSIFFGAVHDF
                     SSLIGSVRHKARSVAEIVKDHIGPRAYKFFLAFIWLTLVYVIASFTDITSSSFAETQF
                     GGGVASSSALYLVIGVAMGFCLYRLKMPLWMATLLFVPLVGLSIWFGQAVPLRMPGLG
                     PCSPQMVWNFIILGYCFLASVIPMWILLQPRGYLGGFFLYGALLAGVVGVFLSGDRVQ
                     YPAFIGFTSLKGLPLFPMLFVTVACGACSGFHGLVSSGTTSKQVAREPDCHLVGYGGM
                     LLEGFVAVVALSTVMLLAPGDVSLSASPDRIYANGLSHFVEQFGIDPDFARSFALLAF
                     ATFIYDTLDVATRLGRYVLQELTGWKGWKGGAAATVLTLILPAYFVSATLTDAAGNAV
                     PAWKLFWTIFGTSNQLLAGLTLLGLTVWLKRTGKPWWMTAVPTVFMMTMTFWSLALTV
                     LPWLRRIFDGQIAWDVIPAVGLVLLALSLMLLWETFAVLRSPNVLETEKVSS"
     gene            5238..5690
                     /locus_tag="P5079_08980"
     CDS             5238..5690
                     /locus_tag="P5079_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY30153.1"
                     /translation="MNFSLKHTILLGWPILSVLLAFSVVSLAIFLQCWTLLRKCRGLV
                     NSVGQSDRKILSHALAVVEQRLAILGTIANAAPFVGLLGTVIGVIRAFHSISTATGSG
                     SGLTLVAGGISEALVSTAAGLAVAIPASMIFNYFTYQSQKIAQDAGLD"
BASE COUNT         1091 a         1933 c         1689 g         1073 t
ORIGIN      
        1 cctggaatac gccgaatggg cggggcggca ggaggccgac gtggtcgtgt ttcccgaact
       61 ctccctcccc gggtacccgc cggaagacct cgtcatgcgc cggcattttc aggacgatac
      121 ccgccgggcg ttgacggaac tggcgaaaaa aatgccggcc ggaccgccgg cgctcgtcgg
      181 tttcaccgag accgccggga ccgatctcca taacgccgcc gcggtccttt tcggcgggaa
      241 gatacgcgcc gtgtaccgaa aacagcgcct gcccaactac ggcgtgttcg acgaaaaacg
      301 ctatttcgcg ccgggcaagg aactgatctt tctgaagatc cgcggcgtct gcgccgggat
      361 caccctctgc gaggacgtct gggccgacga cggcccggcc ccggccctgg ccaagcaggg
      421 ggcgcaggtg atcctcaacc tctccgccag cccctatcac gccggaaaac agtccctgcg
      481 gcggcggacc atcggcgaag tggtaaaaaa atgcggccga ccgctggtct attgcaacat
      541 ggtgggcgga caagacgaat tggtcttcga cggcggcagc atggcgatgg acgcccacgg
      601 acggatcgtc gcggacgccc ctcaattcga ggaatgcctt ctgaccgcgg acatccccgt
      661 gcccccagcc acacgcttcc ctaaaaacat cgtccccttg agcccggcgc ccgccgggac
      721 gaaaatcccg atagaacctc ctccttccgc cgcgccgttg tccgaattgg acgaaatcta
      781 ttcggcgctg gttttgggaa cgcgcgatta cgtgaagaag aacgggttca aaaaagccgt
      841 cctcggcctg tccggcggta tcgattcctc cctggtggcc tgcgtggccg tggacgccct
      901 cgggaaagaa aatgtcatcg gagtcaccat gcccagccgg ttcaattcgc ccgaaacgcg
      961 ctccgacgcg gaaatcctgt ccagaaatct gggaatcggg ttccattccg tccccatcga
     1021 aaccgtcacc gaagagttcc tcaaggtcct tcgccccgtc ttcggcgacc gcccccgcga
     1081 tctggccgag gaaaacctcc aggccagggt gcgcggcacg ctcctgatgg ccctttccaa
     1141 caaattcggc tggatggtgc tcaccaccgg aaacaaatcg gaggtctccg tcgggtacag
     1201 caccctctac ggcgacaccg ccggcggctt cgcggtcatc aaggacatcc ccaagacgct
     1261 ggtctatcgg ctcgcgcggc ggcgcaacga aaaggcgggc cggcaactca tcccccagag
     1321 cgtcttcgac cgccccccca ccgcggaact gcgtccgaac cagaccgacc aggacagcct
     1381 gccgccttac gaactgctgg acaacatcat ccggctctac gtggaagagg acaagggcct
     1441 cggagaaatc gtaaaatccg gcgtgggatc ggagatcgcc gcgaaagtct tgcgcctggt
     1501 cgacgccagc gaatacaaac gccggcaggc cccgccgggc gtgaagataa cgcccaaagc
     1561 gttcggacgc gaccggcgca tgccgatcac caaccgctat cggcccggcc agccgaactg
     1621 atttgaaaag aacggaccca tggacataaa aaacaaagtc atcctgatca ccggcgcctc
     1681 cagcggcatc ggctgggcca ccgccgaagc cctggcccgc gaaggcgccc ggctcgccct
     1741 ggccgcccga cggcaggacc gcctcgacgc gctggcccaa accctgcacg agcggcacgg
     1801 cacggagacc ttcgtcgtcc gcaccgacgt gaaacgggaa aacgaggtcc gctacatgat
     1861 caccgagacc gtcaaacgct tcggccggct ggacgtcctc atcaacaacg ccggggtcct
     1921 cttcatgggg caattcctcg agatgcccct ggtggacatg cacgccatca tggacaccaa
     1981 ctattggtcc atcgtccacg ccgtcaaatt cgccgcgccg ccgatgtcca aacagggcgg
     2041 cgggcacatc atcaacatcg cttcgggcgt cagccgccgc ggactgccct tcatggccgc
     2101 ctacagcgcc tccaaatacg ccctcgccgg cctgaccgaa gccgtccgtt tggaactggc
     2161 cgcgcaaaat atcaaattca ccaccgtcta tcccggcggc gtcgagaccg aaatgccgaa
     2221 aagcgtggac cggaaaaaac tcccccccac ctaccccgac caacgccgct ggcgcatctc
     2281 cgccgaacgc gtggcccggg ccgtggtcaa agccgtgcgc aaacagccgg tggaaatctt
     2341 cgtcccctgg tgggtcggca tcggcccgtg gatcaacctc ttcagcccgg ccctggccga
     2401 ccgcctcatc cgccgcgcgg aaaagatcta agaccccatg gacatcggca tcgtcggcct
     2461 gcccaacgtc ggcaaatcca cgctgttcaa cgcgctcacc ggcgcggccg ccgccgccag
     2521 taattttccc ttcaccacca tcgaccccaa cgtgggcatc gtgcctctgc cggacgaacg
     2581 cctcaacgtc ctcggaaaaa tgttcgcgag cgaaaaggtc acgccggcgg gcgtccgttt
     2641 tgtggacatc gccggactcg tcaagggcgc cagccagggc gaggggctcg gcaaccagtt
     2701 cctctcccac atccgcgccg tggacgcggt ggcccacgtg gtgcgggcct tccacgacga
     2761 gaacgtggtg aacgtgctgg ggaaactgga cccgctggag tccgccgaca tcatcgagac
     2821 cgaactcctc ctggcggacc tccagcaggc gcagaaactt ctggacaaac tccagggacc
     2881 cgcccggtcc ggcgacaaaa aagcgaaaga gcgcctggcc cagatggaac agatcatcaa
     2941 ggggtttcag gaaggcaaat ccgcccgcat gcagaacctg ccggaagacg tgaaccgtga
     3001 atacgagttc ctcacgggca aaccgctcct ctacgtggcc aacgtggacg aacgcggcgg
     3061 cgagccaaca ctgttggctc ctttgacgga aagggcaaaa agagaaaatg ccaaagtggt
     3121 tgtgctgtgc gccaagatcg aggcggagat cgtccaactg gcggaagagg aacgggccag
     3181 cttttatgag tcggcgggaa tcaaacagtc cgggttggcg gaactggccc atgccggaaa
     3241 agacctgttg gacctcatct gtttcttcac ggccggcccg aaggagtcgc gcgcctggat
     3301 cattcccaaa gggaccaaag ccgtcaaagc cgccgggaag atccactccg acatcgaacg
     3361 cggcttcatc cgcgccgaaa tgtacaaata cgacgacctg gtgaagctgg gttcctataa
     3421 agccgttcag gaaaaaggcc tggtctccat ggaaggcaaa gactacgaga tgaaggatgg
     3481 cgacgtcgtc tattttcggt tcaacgtctg attcccgaag gcccgcttaa atgtcccttg
     3541 cgctgatcgc cacggtctgc actctcctct tcgtggccgc gtatttcctt tacggacggc
     3601 tggcggcgag acttttcaaa ctcgacccgc aaacccccac cccggcgaag gtgtatgagg
     3661 acggggtgga cttcgttccc gccaaagcgt cgctcttgct gggacagcat ttctccgcca
     3721 tcgccgccgc cggacccatc gtcggcccca tcctcgccgg gatctggttc ggctggctgc
     3781 cggccctgct gtggatcgtc ctcggctcca tcttcttcgg agcggtgcat gatttttcca
     3841 gcctcatcgg ttccgtgcgc cacaaggccc gctcggtggc cgaaatcgtc aaggaccaca
     3901 tcggtcctcg ggcgtacaag tttttcctgg cgttcatctg gctcaccctg gtgtatgtga
     3961 tcgcctcctt cacggacatc accagttcct ccttcgccga aacgcagttc ggcggcgggg
     4021 tggcgtcctc ctcggccctt tacctggtca tcggcgtggc catgggtttc tgtctctacc
     4081 gtctcaaaat gccgctgtgg atggccaccc tcctcttcgt gcctctggtg ggattgtcca
     4141 tctggttcgg ccaggccgtc ccgctgagga tgccgggcct cggtccgtgc tcgccgcaga
     4201 tggtgtggaa cttcatcatc ctcggttatt gttttctggc ctccgtcatc cccatgtgga
     4261 tattgctcca gccgcgcggt tatctcgggg gattcttcct ttacggggcg ctgctcgccg
     4321 gcgtggtggg cgttttcttg agcggcgacc gcgtgcagta tcccgccttc atcggattta
     4381 ccagcctgaa aggccttccg ctttttccca tgctcttcgt gacggtggcc tgcggcgcct
     4441 gttcggggtt ccacgggctg gtgagttccg gaaccacgtc caaacaggtg gcccgggaac
     4501 ccgactgcca tctggtggga tacggcggca tgttgctgga aggattcgtg gcggtggtgg
     4561 ctctctccac ggtgatgctg ctggcgccgg gagatgtttc gctctccgcc tcgccggacc
     4621 gcatctacgc caacgggctc agccatttcg tggaacagtt cggcatcgac ccggacttcg
     4681 cccggtcctt cgccctgctc gccttcgcca ccttcattta cgacacgctg gacgtggcca
     4741 cccgcctggg ccgttacgtg ctgcaggaac tgacgggctg gaaaggatgg aagggcggcg
     4801 cggcggccac cgtgctgacg ctgatcctgc cggcctattt cgtcagcgcg acgctgaccg
     4861 acgccgccgg gaacgccgtc cccgcctgga aactcttttg gaccatcttc ggcacgtcaa
     4921 accagttgct ggccgggctg acactcctgg ggctcaccgt ttggctgaaa cgcaccggga
     4981 aaccctggtg gatgacggcc gtccccaccg tgttcatgat gaccatgacc ttctggtcgt
     5041 tggcactgac ggtgctgccg tggctgaggc ggattttcga cggccaaatc gcatgggacg
     5101 tgatcccggc ggtggggctg gtccttttgg ccctgtccct gatgctgttg tgggaaacgt
     5161 tcgccgtgtt gcgctcgccg aatgtccttg aaacggagaa ggtttcttcg taaagtgtct
     5221 gtcggaggaa aaaacggatg aacttcagtc tcaaacacac gatcctactg ggctggccga
     5281 tcctgagcgt cctgctggct ttttccgtcg tgtcgctggc catttttctt cagtgctgga
     5341 ccctcctgcg gaaatgtcgg gggctggtca attccgtcgg ccaatccgac cggaagatac
     5401 tgtcccacgc gctcgcggtc gtggagcaac gtctcgccat tttgggcacc atcgccaacg
     5461 ccgcgccctt cgtcgggctg cttggaacgg tgatcggcgt gatccgggcc ttccattcca
     5521 tctccaccgc caccggctcc ggcagcggac tcacgctggt ggcgggcggc atctccgaag
     5581 cccttgtgag caccgccgcg ggactcgccg tggccatccc ggcctccatg atcttcaact
     5641 atttcaccta ccagagccaa aagatcgccc aggacgccgg gctggactga cgcttttccg
     5701 gccgccgatt tcgccatggc cacttcccat cacctcttca taaaaaaacg tctcttcacc
     5761 gagatcaaca tggtgccgtt catcga
//
LOCUS       DAOTFD010000169         5660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10231_length_5660_cov_5.922569, whole genome shotgun sequence.
ACCESSION   DAOTFD010000169 DAOTFD010000000
VERSION     DAOTFD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10231_length_5660_cov_5.922569"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="P5079_08985"
     CDS             <1..489
                     /locus_tag="P5079_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRY30154.1"
                     /translation="DREASGLVLFAKDAEAHRDLSLQFEERRVKKIYLAVAEGRLEKN
                     VTVDQPLRLFGSGRVGVDSRGKASTTVFHVKELFKNATFVEAEPLTGRRHQIRAHLYW
                     LGHPLLGETRYGKDFPVGGTSGLMLHGHRLTFLGPDKKPLTLRAEPSDFFLRVLDGLR
                     RS"
     gene            486..1085
                     /locus_tag="P5079_08990"
     CDS             486..1085
                     /locus_tag="P5079_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30155.1"
                     /translation="MKPPASASLALCLASPKEAAEFCRRAEKTAGLAGNILLTGYRNA
                     SLDTVPPQKWAGDVLAAGFAGALRPTLDPGDLLLAERVCSEDAGVLETSASLTRSIAT
                     ALREENIPYVTGTLLTVSRAVTGASKKRELRGKGFDAVDMESHGLLAHGRGRIAALRL
                     ISDRADETAASDYAAARDALSEKFSRVLGRVFDRLSRSS"
     gene            1082..2056
                     /locus_tag="P5079_08995"
     CDS             1082..2056
                     /locus_tag="P5079_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRY30156.1"
                     /translation="MKPGPVRTVPLGKIVATESPFRLTPLENRPSEILLESIRRVGLL
                     TPLWVKPAGPRYVLVAGFRRYAAARTLRFRTVPAIAFSNRSDAELLRWAAHDNAGARQ
                     LTLLEKLAQVRALRAFGVPQAELVKSDFPRLGFPPLPRFIDTLKKMETLGDGLLSYLS
                     EKDAPLRRLTSFFGLDRPAADLLARLAQSFRPGLNDLEAFARCLIETASRDGEKPSQL
                     LREIERAIPPQSPDRPSHETFSLFAAELFRRRFPTLSLHREKAARLLRRLRLPDFVKL
                     NWDPSFERPGVSLRAEIQNPQQWQELLRRLNRPGTAETFRRLLKLTDP"
     gene            2064..3227
                     /locus_tag="P5079_09000"
     CDS             2064..3227
                     /locus_tag="P5079_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30157.1"
                     /translation="MRRPPIKLFLVDESVRHRPNVCAFLRNYPRVPVKIVPAGQTIQA
                     LLTKKNISPDRTVYSLAEPKGKNLKKCQGMTEHYLCCQLHILDQSENCPFSCSYCFLQ
                     NYLSQSITAISMRFDPLFKEVQETVGAEPRRLFRIGTGELSDSLALDPLTGFSRKAVP
                     FFSSLPNALLELKTKSDRVEQLLNLHHGGRTVISWTLNPPAVARREERGAASPSARLE
                     AARKTAAAGYKIAFHFDPLIHYPGFEKDYALLVRQMFDAVPVEKIAWVSLGSLRYPPE
                     MQQTVLDRFPGSGIAVGEMVRGYDGKMRYFKPIRHRLYETLRAALEGGGAGLFLYFCM
                     ENAETWRKFLGWAPKNNADFDFRFARHLCRKFPGLMPAPPKPDDYRVAPDPAA"
     gene            complement(3232..3504)
                     /locus_tag="P5079_09005"
     CDS             complement(3232..3504)
                     /locus_tag="P5079_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30158.1"
                     /translation="MHYICIVRDTFKRHNTVELAASLAVIEQRLEKENRARALREKPG
                     EVLLVYYECLPTLAEAKKRKEHLLSASGIEEAQKWLQRWDRAEVID"
     gene            complement(3509..4828)
                     /gene="serS"
                     /locus_tag="P5079_09010"
     CDS             complement(3509..4828)
                     /gene="serS"
                     /locus_tag="P5079_09010"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY30159.1"
                     /translation="MLDIKLIRNQPETVRAGLQSRGGRYLPAFEELLSADAEWRKVNA
                     EADALRARRNQAADEIGRLKKEKKDIGGVLKEMEGVKATLRELEERAKALEGRVQESL
                     LNIPNLPDSSVPAGKEPSDNKVVREWGTKRTFAFSARDHQDVGEGLGILDFGRAAKLS
                     GARFALLVGEGARLERALIQFMLDLHTREHGYTELFPPFLVNRATMTGTGQLPKFAEE
                     LYSCPSDDLFLIPTAEVPVTNIFRDEVLEEAALPRAFCAYTACFRREAGSYGKDTRGL
                     IRNHQFNKVELVRFCRPEDSLAQLEILTGHAEEVLKRLNIPYRVMALCAGDLGFSSAK
                     TYDLEVWMPGDRSGGESGGRPNGAWREISSCSTFTDYQSRRIGIQMKRSDGPRALPHT
                     LNGSGVAVGRTMAAVLENYQEPDGGVLVPEALRPYLGTDRLKPAKYF"
     gene            4916..5542
                     /locus_tag="P5079_09015"
     CDS             4916..5542
                     /locus_tag="P5079_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30160.1"
                     /translation="MRTLRPPLRMLFLTAPLVLVAGLGAGAAEKPLSVSYEQVLTTAK
                     GSFVTKVWIKGDKKRVETADGREIDIVQGNTYVQIFPEQGFARRMPLAAAPSAPMPAA
                     ANKVSADGKTTTAAGTKPKNTDMVADLPCKLYELKSGEGVTRIWISDRLPFPLKWEEV
                     DPGAGKFSYEVKNLKFADDIPEEYFQVPSGFKIVNAEPDDLPPELKNP"
     gene            complement(5553..>5660)
                     /locus_tag="P5079_09020"
     CDS             complement(5553..>5660)
                     /locus_tag="P5079_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRY30161.1"
                     /translation="APMAYKDVENVMDVCDGAGLSKKVARLRPLAVVKG"
BASE COUNT         1053 a         1909 c         1636 g         1062 t
ORIGIN      
        1 gacagagaag ccagcgggct ggtccttttc gccaaggacg ccgaggccca tcgagacctc
       61 tccctccaat tcgaggaacg ccgggtaaaa aaaatctatc tggccgtggc ggaaggccgc
      121 ttggaaaaaa acgtgaccgt ggaccagccc ctccgcctgt tcggatcggg acgggtgggg
      181 gtagacagcc gcggcaaggc ctccaccacc gtcttccacg taaaggaact cttcaaaaac
      241 gccaccttcg tggaagcgga accactcacg ggtcgacggc accagatccg cgcccatctt
      301 tactggctcg gccatcccct ccttggggaa acgcgctacg ggaaggattt ccccgtcggc
      361 ggaacctccg ggctgatgtt gcacggccac cggttgacgt tcctcggacc ggacaaaaaa
      421 ccattgaccc tccgggccga gccgagcgat tttttcctgc gggttctgga cggtcttcgc
      481 cgatcatgaa accgccggct tcggcatctc tcgccctttg cctcgcctcg ccgaaagagg
      541 cggcggaatt ctgccgccgc gccgaaaaga ccgcggggct cgccgggaac atccttttga
      601 ccggataccg taacgcctcc ctcgacaccg tgcctcccca aaaatgggcg ggcgatgttc
      661 tggcggccgg gttcgccggc gccctgcgcc cgacgttgga cccgggagac ctcctcctgg
      721 ccgaacgcgt ttgttcggaa gacgccggcg tcctggagac ctccgcctcg ctcacgcgtt
      781 ccatcgcaac ggccctgcgg gaggagaaca tcccctacgt caccggaact cttctgaccg
      841 tgtcgcgggc cgtgaccggc gcttctaaaa aaagagagct ccgcgggaaa gggttcgatg
      901 ccgtggacat ggagtcccac ggcctcctcg cgcatgggcg gggccgaatc gcggccctcc
      961 ggctcatcag cgaccgggcc gacgagacgg ccgcatcgga ttacgccgcg gcccgggacg
     1021 ccctctccga aaaattcagc cgcgtcctcg ggcgcgtgtt cgaccggctt tcgaggtctt
     1081 cgtgaaaccg ggccccgttc ggaccgtccc tctgggaaag atcgttgcga ccgaaagccc
     1141 gtttcggcta accccgctgg aaaaccgtcc ttccgaaatc ttgttggagt ccatacgtcg
     1201 cgtcggccta ctgacgcctc tctgggtgaa acccgccggc ccgcgttatg tgctggtcgc
     1261 cgggttccgg cgttacgcgg cggcaagaac gttgaggttc cggaccgttc cggcgatcgc
     1321 tttctccaat cgttccgacg ccgaacttct ccgttgggcg gcccacgaca acgccggagc
     1381 gcggcagctc acgctgctcg agaaactcgc ccaggtgcgc gccctccgcg ccttcggcgt
     1441 cccacaggcg gaactcgtca aatccgactt cccccggctg ggtttcccgc cgcttccgcg
     1501 gttcatcgac accctgaaaa aaatggagac gctgggcgac ggcctgctct cttatctttc
     1561 cgaaaaagac gccccgctcc gccgtctcac ttcttttttt gggttggacc ggcccgccgc
     1621 cgacctcctt gcgcgcctgg ctcagtcgtt ccggcccggg ctgaacgacc tggaggcctt
     1681 cgcccgatgc ctgatcgaga ccgcctcgcg cgacggagaa aaaccgtctc agctcctccg
     1741 ggaaatcgag cgggcgatcc cgccgcaatc cccggatcgc ccgtcccacg agacgttctc
     1801 cctgttcgcc gccgaacttt tccgacggcg cttcccaacg ctttctctcc accgggaaaa
     1861 agccgcccgg ctcctgcggc ggctccgtct gccggatttc gtcaaactca actgggaccc
     1921 ctccttcgag cggcccgggg tctcccttcg ggcggagatc caaaatccgc aacagtggca
     1981 ggaactgctc cgccggctga accgcccggg aacggccgaa acgttccgcc gtctcctgaa
     2041 actgacggac ccctaagaac gtcatgcgtc ggccccccat caaattgttc ctcgtggacg
     2101 aaagcgtccg gcatcgaccc aacgtctgcg ccttcctccg gaattatccg cgcgttccgg
     2161 tgaaaatcgt cccggccggc cagacgatcc aggcgctgtt gaccaaaaaa aatatttccc
     2221 cggacagaac cgtctattcc ctggccgaac cgaaagggaa aaacctcaaa aaatgccagg
     2281 gcatgaccga gcattatctt tgttgccagc tccacattct ggatcaatcg gaaaattgcc
     2341 cgttctcctg ctcctattgc ttcctgcaaa actacctgtc gcaaagcatc acggcgatct
     2401 ccatgcggtt cgatccctta ttcaaggaag tccaagaaac cgtcggggcg gaaccgcggc
     2461 ggcttttccg catcggaacg ggggaattga gcgacagttt ggcgttggac ccgctcaccg
     2521 gtttttcccg gaaggccgtc cccttttttt cttccctccc caacgcgctc ctcgaactca
     2581 agacgaaatc cgaccgcgtg gaacagctcc taaacctgca ccacggaggg cgaaccgtca
     2641 tttcctggac gttgaacccg ccggcggtgg cgaggcgaga ggaacggggc gccgcctccc
     2701 cctcggcgcg gctggaggcg gcccgaaaaa ccgcggcggc gggctacaag atcgcttttc
     2761 atttcgaccc cctcattcat tatcccgggt tcgaaaaaga ttacgccctc ctggtgcggc
     2821 agatgttcga cgccgtcccg gtggaaaaaa tagcctgggt cagcctgggc tctctccggt
     2881 atccgccgga gatgcaacag accgttctgg accgttttcc cggatccggg atcgctgtgg
     2941 gcgagatggt gcgcggctac gacgggaaga tgcggtattt caagccgatc cgccaccgtc
     3001 tttatgagac gctgcgggcc gcgctggaag gcggcggggc ggggttgttc ctctattttt
     3061 gcatggaaaa cgcggaaacg tggaggaaat tcctgggatg ggctcccaaa aacaacgcgg
     3121 acttcgattt tcgattcgcc cgccatctct gccggaagtt cccgggcctg atgccggcgc
     3181 cgccgaaacc ggacgactac cgcgtcgccc cggatcccgc ggcctgaaga atcagtcgat
     3241 cacttccgcc cgatcccacc gctgcagcca cttctgcgcc tcttctattc cgctggcgga
     3301 gagcaggtgc tccttgcgct ttttcgcttc ggccaaggtc ggcaggcatt cgtaatagac
     3361 gagcagcact tctcccggtt tttcccggag ggcccgggct cggttttcct tttccaggcg
     3421 ctgttcgatc acggccaaac tcgccgcaag ctcgaccgtg ttgtgcctct taaacgtgtc
     3481 ccggacaatg cagatgtaat gcatggagtt aaaagtattt cgccggcttc agccggtcgg
     3541 tccccagata gggacgcagg gcttcgggga caagcacgcc gccgtccggc tcctgataat
     3601 tctccaagac cgcggccatg gtgcggccca ccgccacgcc cgaaccgttc agcgtgtgcg
     3661 gcagcgcccg gggcccgtcg gaacgtttca tctggatgcc gatgcggcgg gactggtaat
     3721 cggtgaaggt ggaacaggac gaaatctccc gccaggcgcc gttcggccgg ccaccggatt
     3781 ccccgccgga ccggtctccc ggcatccaca cctccagatc gtacgttttg gcggaagaaa
     3841 acccgaggtc tccggcgcac agcgccatca cgcgataggg gatgttcagc cgcttcaaaa
     3901 cctcttccgc gtgtcccgtg aggatttcca actgcgccaa cgaatcctcc ggccggcaga
     3961 agcggaccag ttccaccttg ttgaactgat ggttccggat gagcccgcgg gtgtctttgc
     4021 cgtaggaacc ggcctcccgt cggaaacagg cggtgtaggc gcaaaaagcg cgcggcaggg
     4081 ccgcctcctc cagaacctcg tcccggaaga tgttggtcac cggcacctcc gccgtgggga
     4141 tgaggaacag gtcgtcgctg gggcaggaat agagctcttc ggcaaatttg gggagctgcc
     4201 cggtgccggt catcgtggcg cggttcacca ggaagggcgg aaaaagttcg gtgtatccgt
     4261 gttcgcgtgt gtgcagatcc agcatgaatt ggatgagcgc gcgctccaac cgggccccct
     4321 ccccgaccaa cagggcgaac cgggccccgg acagtttcgc cgcgcggccg aaatccagaa
     4381 tgccgagtcc ctcccccacg tcctgatggt cccgagcgga aaacgcgaac gtccgcttcg
     4441 tcccccactc ccgcacaacc ttgttgtcgg acggttcctt ccccgcgggg acggaggaat
     4501 ccggaaggtt ggggatgttc aaaagacttt cctgaacgcg gccttcgagc gctttggcgc
     4561 gctcctccaa ttcccgaagg gtcgccttga cgccctccat ttccttgaga acgccgccga
     4621 tgtccttctt ctcttttttc agacgtccga tctcgtcggc cgcctgattg cgccgcgcgc
     4681 gcagggcgtc ggcctcggcg ttgaccttgc gccattcggc gtccgcggaa agaagctctt
     4741 cgaacgccgg cagataccgc ccgccgcggc tctgcaggcc cgcgcggacc gtttccggct
     4801 gattccggat gagttttata tcgagcatgg cccgctcata ttatcacaat taccgtcagg
     4861 attaaagccg acggacatct tgccatcctc ccgctaaaag agtaaatttt ccgtcatgcg
     4921 cactctccgc ccgcctctcc ggatgctgtt tttgacggcg cccctcgtcc ttgtcgcggg
     4981 actgggggcc ggcgccgccg aaaaacccct ttccgtctcc tacgaacagg tcctcaccac
     5041 cgccaagggc tctttcgtca ccaaggtgtg gatcaagggg gacaaaaagc gggtcgagac
     5101 ggccgacggc cgcgaaatcg acatcgtgca gggaaacacc tacgtccaga tctttccgga
     5161 gcaggggttc gcgcggcgga tgcctctggc ggcggcgccc tcggccccta tgccggccgc
     5221 ggccaacaag gtctccgcgg acggaaaaac cacgaccgcc gccggaacaa aaccaaaaaa
     5281 taccgacatg gtggccgacc tgccgtgcaa actctatgag ttgaagagcg gcgaaggggt
     5341 cacgcgcatt tggatatcgg accggctccc gttcccgctg aaatgggaag aggttgaccc
     5401 cggagcgggc aaattttcct acgaggtcaa aaacctgaag ttcgccgacg acatcccgga
     5461 ggagtatttc caggtcccct ccggattcaa gatcgtcaac gccgaacccg acgatcttcc
     5521 ccccgaacta aaaaacccct agcctttcgg cgctaccctt tgacgacggc gagggggcgc
     5581 agccgcgcca cttttttcga aagaccggcc ccgtcgcaga cgtccatcac gttctccaca
     5641 tccttatagg ccatcggcgc
//
LOCUS       DAOTFD010000170         5635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10287_length_5635_cov_3.301971, whole genome shotgun sequence.
ACCESSION   DAOTFD010000170 DAOTFD010000000
VERSION     DAOTFD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10287_length_5635_cov_3.301971"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5635
                     /locus_tag="P5079_09025"
     CDS             <1..>5635
                     /locus_tag="P5079_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30162.1"
                     /translation="VDQLMDSFDGLRMEFSNADGEVIGGMVFNKTSDGSIEVRKLDVT
                     STTDPETGETTTTVTEGAVIGKFTSDQLNSGSARLTFGVGADGKSVVSFSVDIVQNGA
                     RIGQLNIAISVDDTGKQTTSYAVNFVGKYAVELMMDALGVTTVDALMAKLDGVTVEAK
                     DAAGNVIGGYQFNVENGKVQILTLDGEEIVSFTADQLDLGNASLTIGMSSDGSTILAL
                     AIDIRQDGKVIGSFMINLVKGTDGIERLSYLVTFKGEMSLIYLMRALGINPEDPGART
                     ALFNKLDGSKLVISQAGSQVGGYGIDFDEASGTVKIYDLKKNDAGQWVLGAEIGSFNL
                     AEADWSTATISFGIGGDGEYTLGFSLEFKRNGQTVGTFNVSMAKDKDGIQRVNYAIGL
                     QGAFAREVLMKILGIDPDSAGANSELWEKLDGAEMIAYDKNGHVKGGYRLRVEGDNVY
                     VTMLKADPDDSTKAVETGEEFKFGLSSAQWTTASLTLGQSSDGSSLFGFSVDLLDSAG
                     NAFGTFGISMSEGDDGEQVVSYALTFKGQHALKILMEAMGVDSQEALMAALDDVQMVA
                     KDKDGNVLGGYAFHVDDNGTVHVMKLEADPNDPTKMVEGEELASFGLNDIDWKTATLT
                     IGRTSGGDTLFAFSASIRQNGRVIGTFTVSASEVDGRMVFSAAVTFTGEFALNLLMSI
                     LGVKSVDALMAKLDGVQMVVQNDAGEVTGGYEIDIVNGRVSIYALEADPEDSTKAVRR
                     AGGAIGTFAVSQINWASCSLTLGTAIGGDAVMAFSADVRAADGNKIGTFNVSMALDEK
                     GIMRLNYMLNFTGQYALDLLMDVLGVDTREELLEKLDGSNLVVKDGDGKIIGGYSVEV
                     ENGKVTIYKLNVSEAGVVNKDVMGPVLMSFDMGSIDWGTVTLSMGTSSDGEELFGFSA
                     DIRQNGRVIGRFSISIGGEEDQREFSFSLTFTGERARELFMKILGIDPAAAGAERQLW
                     AKLNGAEMVAYDKKGNVVGGYRLEVAGDTVHIIKLKADPKDSTKTVDDGEIGVFDMSN
                     VNWDTASFTIGQTGTGGAIMAFSVDIRDASGRALGIFSVGVTEDAAGERTVNYSMTFK
                     GAYAVELLMKVMGVTSREALVAALNGVQMVAKNKEGEVIGGYSFRVDADGTVHVMKLR
                     ADPNDNTKAVEDSELFSFNMGDIAWDTATFTVGRSSNESTIFAFSAALRRNGVEIGTF
                     SITSSENAQGQTVLNIAVTFTGQFALDLLMDILGVSTKAELVAKLDGAQMLVLDANGD
                     VTGGYEIDVTGNRIQIYRLKANPQDHSKAVRDGAAMMTFGLDQIQWATSTLTLGVSLG
                     GDAVLAFSADIHDAQGIKIGTFSISLTQDSKGYTQYNYLLSFTGAFATEMLMKVLGVT
                     TYAELMQKLDGSKLVAVGGDGKVLGGYEVDVNPDGVVTIYKLVAGANGEAVRAKDEAG
                     NEVALMRFDQGAIDWASATLTLGISSTGTTTFSFAADIKVGGELIGRFGVSMAGEGQK
                     QYLNFAVAFTGKTAFRLLMQILNITGAENPARVLQNMLKDVMMKFEREMEGDDPRTDE
                     VEGEDNKYTLIEELSLSDFLGIGTADELTFGFDEVSLTIGMNSKGQTVFAFTVDLLAA
                     RSGEKIGQISIAWDAGYAAFSYTFSLNLDSPVARHIFINRFMDILHVRTYDQLVQALS
                     NITFTDPNGNEVNLGDVIRGVIDGTVSGTFTFGVTSQGTAVFSFTLQLANGRQIQGVV
                     TGDMKLSTSYLVNGDVREVYSVDLLKKSAGGTFDVTSHYALHAGQIVQLPDESWGFVD
                     GHQLTEAEARGLNDKAQSLGAAITNLTYNKDTNEVLATASGPNGTTYTIGVRAGTGVK
                     FGRFIGAESCNFGAIDGVVADDEGNLSTKEVAIKAA"
BASE COUNT         1152 a         1668 c         1833 g          982 t
ORIGIN      
        1 acgtggacca gttgatggat agtttcgacg gattgcggat ggagttttcg aacgcggacg
       61 gcgaggtgat cggcggcatg gtgttcaata agaccagcga cggttccatc gaggtccgca
      121 agctggacgt gacctccacc accgatcccg agacggggga gaccaccacc acggtgacgg
      181 agggcgcggt catcggcaag ttcacctcgg accagctcaa ttccggcagc gcccggctga
      241 ccttcggggt cggggcggac ggcaaatccg tggtgtcgtt cagcgtggac atcgtgcaga
      301 acggggcccg catcggacag ctcaacatcg ccatctccgt ggacgacacc ggcaagcaga
      361 ccaccagcta cgcggtcaac ttcgtcggca aatacgcggt ggaattaatg atggacgctt
      421 tgggcgtcac caccgtggac gccctgatgg cgaaactgga cggcgtcacc gttgaggcca
      481 aggacgccgc cggcaacgtc atcggcggct accaattcaa cgtggagaac ggcaaggttc
      541 agatattgac tttggatggg gaggagatcg tttccttcac cgccgaccag ctcgacctcg
      601 gcaacgcttc gctaaccatc ggcatgtcgt ccgacggttc gaccatcctg gccttggcca
      661 tcgacatccg ccaggacggc aaagtgatcg gctcgttcat gatcaacctg gtgaagggga
      721 cggacggcat cgaacgcttg agttacttgg tgacctttaa aggagagatg tccctcatct
      781 atctcatgcg ggccctgggg atcaatccgg aggaccccgg ggcgcgcacc gcgctcttca
      841 acaagctgga cggttccaag ctcgtcatca gccaggccgg atcgcaggtc gggggctacg
      901 ggatcgactt cgacgaggcc agcggcaccg tgaagattta cgacctgaag aagaacgacg
      961 ccggtcagtg ggtgctcggc gcggagatcg gcagtttcaa cctggcggag gccgactgga
     1021 gcaccgccac catcagtttc gggatcggcg gcgacgggga atatacgctc ggtttctcgt
     1081 tggagttcaa gcggaacggc cagacggtgg gcacgttcaa tgtctccatg gccaaggata
     1141 aagacggtat tcagcgggtc aattacgcca tcggccttca gggggcgttc gcccgcgagg
     1201 tcctcatgaa gatcctgggc atcgacccgg attcggcggg cgccaactcg gaactgtggg
     1261 agaagttgga cggggcggag atgatcgcct acgacaagaa cggccacgtg aaaggcggct
     1321 atcggctccg ggtggagggg gacaacgtct acgtcaccat gctgaaggcc gatccggacg
     1381 attccaccaa ggcggtggag acgggcgagg aattcaagtt cggcctctcc agcgcccagt
     1441 ggacgaccgc ttccctgacc ctgggacagt ccagcgacgg gagttccctg ttcgggttca
     1501 gcgtggacct gttggatagc gccgggaacg cgttcggcac tttcggcatc agcatgtcgg
     1561 agggcgatga cggggagcag gtcgtcagtt atgcgttgac cttcaaggga caacacgcgt
     1621 tgaaaatttt gatggaggcc atgggcgtcg attcgcagga ggcgctgatg gccgctctcg
     1681 atgatgttca gatggtggcc aaggacaagg atgggaacgt cctgggcggc tatgcgttcc
     1741 atgtggacga caacggcacg gtgcatgtga tgaaactcga ggccgacccg aacgacccca
     1801 cgaagatggt ggaaggggag gaactggctt cgttcgggtt gaacgatatc gactggaaga
     1861 ccgccacgct gaccatcgga cgcacctccg gcggcgacac cctctttgcc ttttccgcct
     1921 ccatccgcca gaacgggcgg gtgatcggca ccttcacggt cagcgcttcg gaggtggacg
     1981 gccggatggt cttcagcgcg gcggtgacgt tcaccggcga gttcgcgttg aatctgttga
     2041 tgagcatcct cggggtgaaa tccgtcgatg cgctgatggc caaattggac ggcgtgcaga
     2101 tggtggttca gaacgacgcc ggggaggtca ccggcggcta tgagatcgac atcgtgaacg
     2161 gccgggtctc catctacgcc ctggaagccg atccggagga ttcgaccaag gcggtccgcc
     2221 gggccggcgg ggccatcggc acgttcgccg tgagccagat caattgggcc tcctgctccc
     2281 tcacgctggg caccgccatc ggcggcgacg ccgtgatggc tttctcggcc gacgtccgcg
     2341 ccgccgacgg gaataagatc ggcaccttca acgtctccat ggcgctggac gagaagggga
     2401 tcatgcggct gaactatatg ctcaacttca ccggccaata cgccctggac ctcctcatgg
     2461 acgtgctggg ggtggacacc cgggaagaac tgctggagaa gctggacggc tcgaatttgg
     2521 tggtgaaaga cggcgacggg aagatcatcg gcggttacag cgtggaagtg gagaacggca
     2581 aggtgaccat ctacaagctc aacgtctccg aggcgggggt ggtgaacaag gacgtgatgg
     2641 ggcctgtcct gatgagtttc gacatgggtt ccatcgattg gggcaccgtg accctcagca
     2701 tgggcacctc ttccgacggg gaggagctct tcgggttctc cgccgatatc cgccagaacg
     2761 gccgcgtcat cggccggttc tccatcagca tcggcgggga ggaggaccag cgggagttca
     2821 gcttctccct caccttcacg ggcgagaggg cccgggaact cttcatgaag attttgggaa
     2881 tcgacccggc cgcggcgggc gcggaacggc agttgtgggc caaactcaac ggcgcggaga
     2941 tggtggccta cgacaagaag ggcaacgtgg tgggcggcta tcggctggag gtcgccggag
     3001 acaccgttca tatcatcaaa ttgaaggccg atccgaaaga ttccaccaaa accgtggacg
     3061 acggggaaat cggcgtcttc gacatgagca acgtcaactg ggacaccgct tctttcacca
     3121 tcggccagac cggaacgggc ggagcgatca tggccttctc cgtggacatt cgtgacgcca
     3181 gcggccgcgc cctggggatt ttcagcgtgg gggtgaccga agacgccgcc ggggagcgga
     3241 ccgtcaacta ctccatgacg ttcaagggcg cgtatgcggt ggaactgctg atgaaagtga
     3301 tgggcgtcac cagccgagag gccctggtgg cggccttgaa cggcgtgcag atggtggcca
     3361 aaaacaagga aggcgaagta atcggcggct attctttccg ggtggacgcc gacggcaccg
     3421 tccatgtgat gaagctgagg gccgatccga acgacaacac caaggccgtg gaagattcgg
     3481 aactcttttc cttcaatatg ggcgacatcg cctgggacac cgccaccttc accgtgggcc
     3541 ggtcgtccaa cgagtccacg atcttcgcgt tctcggccgc gttgcgccgg aacggcgtgg
     3601 agatcggcac cttctccatc acctcctcgg aaaacgcgca gggccagacg gtgttgaaca
     3661 tcgcggtgac gttcaccggc caattcgccc tcgatctgct gatggatatt ttgggggtct
     3721 ccaccaaggc cgaactggtg gcgaagctgg acggcgccca gatgctggtg ttggacgcca
     3781 acggcgacgt gaccggcggt tacgagatcg acgtcaccgg aaaccgaata cagatttacc
     3841 ggctgaaagc caacccgcag gaccattcca aggcggtgag ggacggcgcc gcgatgatga
     3901 cgttcggcct cgaccagatc cagtgggcca cctccaccct caccctgggc gtgtccttgg
     3961 gcggcgacgc cgtgctggcg ttctcggcgg atatccacga cgcgcagggc atcaagatcg
     4021 gcactttctc catcagcctg acgcaggatt ccaaaggcta tacccaatac aactacctcc
     4081 tcagtttcac gggcgccttc gccaccgaga tgttgatgaa ggtcctggga gtgaccacct
     4141 acgccgagct gatgcagaaa ctggacggct ccaaactcgt ggcggtggga ggcgacggca
     4201 aggtcctggg cggctacgag gtggatgtga acccagacgg cgtggtgacc atctataaac
     4261 tggtggcggg cgccaacggc gaagcggtgc gggccaagga cgaagccggc aacgaggtgg
     4321 cgttgatgcg gttcgaccaa ggcgccatcg actgggcctc cgccaccctg accctcggca
     4381 tctcctccac cggaaccacc acgttctcct tcgccgcgga catcaaggtc ggcggggaac
     4441 tcatcggccg cttcggcgtg agcatggcgg gcgaagggca gaaacagtac ctcaatttcg
     4501 ccgtggcgtt caccggcaag accgctttcc ggctcctcat gcagatcctc aacatcacgg
     4561 gcgcggagaa ccccgcccgg gtcctccaga acatgctgaa ggacgtgatg atgaagttcg
     4621 agcgggagat ggagggggac gacccgcgga cggacgaggt ggagggcgaa gacaataaat
     4681 ataccctcat cgaggaactg tcgctgtcgg acttcctcgg catcggcacc gccgacgaat
     4741 tgacgttcgg tttcgacgag gtctccctga ccatcgggat gaacagcaag gggcagaccg
     4801 tcttcgcctt tacggtggac ctgctggccg cccgcagcgg agagaagatc ggacagatct
     4861 ccatcgcgtg ggacgcgggc tacgcggcgt tcagctacac cttctctctc aacctggaca
     4921 gcccggtggc ccggcacatc ttcatcaacc ggttcatgga catcctccac gtccgcacct
     4981 acgaccagct cgtccaggcg ctcagcaaca tcaccttcac cgatcccaac ggcaacgagg
     5041 tcaacctcgg ggacgtcatc cggggcgtca tcgatggaac cgtctcgggc accttcacct
     5101 tcggcgtcac ttcgcagggc accgctgtgt tctccttcac gctccagctg gccaacggac
     5161 ggcagatcca gggcgtcgtc accggggaca tgaaactttc gacgagctat ctcgtcaacg
     5221 gcgacgtgcg ggaagtctat tcggtggacc tgttgaagaa gtcggccggc ggcacgttcg
     5281 atgtgacctc tcactacgct ctccacgccg gacagatcgt ccagctgccg gacgaatcgt
     5341 ggggttttgt ggacggccat cagctcaccg aggcggaagc gagaggcctg aacgacaagg
     5401 cccaaagtct cggcgccgcc atcaccaacc tcacgtacaa caaagacacc aacgaagtcc
     5461 tcgccacggc gtcggggccc aacggcacca cctacaccat cggcgtccgg gccggcaccg
     5521 gcgtcaagtt cggccggttc atcggtgccg agagctgcaa cttcggggcc attgacggcg
     5581 tggtggccga tgacgagggc aacctgagta ccaaggaggt ggccatcaag gctgc
//
LOCUS       DAOTFD010000171         5628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10304_length_5628_cov_5.035528, whole genome shotgun sequence.
ACCESSION   DAOTFD010000171 DAOTFD010000000
VERSION     DAOTFD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10304_length_5628_cov_5.035528"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5079_09030"
     CDS             complement(<1..642)
                     /locus_tag="P5079_09030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY30163.1"
                     /translation="MKSRPFGKAGFDVSEIGFGSWGIGGTWWGRSIDDKSSLKALHRA
                     LELGITFFDTALVYGKGHSEQLIARAFREKKPTNVYVASKIPPKNNGWPARHDTLARQ
                     VFPKDWIVSCLDKTLRNLSLERIDLIQFHVWSDSWMREDEWWETVESLKKTGKIRLWG
                     VSINDHEPESALELVRSGRADSVQVIYNIFDQSPAEKLFPLCLEKNTAVIVRVP"
     gene            complement(639..1514)
                     /locus_tag="P5079_09035"
     CDS             complement(639..1514)
                     /locus_tag="P5079_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRY30164.1"
                     /translation="MIEATLEKDLSPYIKPVSLRLLSLSAGLLALTASLMAASVWFVK
                     KESLRSFSARSAKLTHWVQTNSQEIIDQHLQGLATIAQPFAAGAADAPAHFQPSAADI
                     LKKCPAFTAINHINQDFIIDDVHPLAANRNALRLDIKSRFDVLPVAHRAITHRKTSVT
                     DFIDLVQGGKGVILYEPIVREERWAGFIEGVFRLNDFNALFLEKFAGPDYNFTIIDET
                     NGREIYTSLPSAAVERSTPFDTYFTMRLGDRTWWVILHPKSPPLILLPLVFVLLLEIC
                     LGAFGLYWLWKSEHA"
     gene            complement(1775..2956)
                     /locus_tag="P5079_09040"
     CDS             complement(1775..2956)
                     /locus_tag="P5079_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3808 domain-containing protein"
                     /protein_id="HRY30165.1"
                     /translation="MTPRRLLPVLLPLLLLALPPGLRAAAASPFQDPEMARPIDDGLR
                     ALYDLDYEAARQNFTVLAEKFPDDPIGQYALTTTHWWELTNEFDEQNPALEKKFIEAA
                     ERTVVIAREKIRRGDPGGEAHLCLGGALGLMSRWDAIQGHWFRAYRHGKQAFNAQEKA
                     LKINPQLHDAGLGVGIFHYYTATLPRVVKLLAKLVFGGNKEQGLAEIRRAMTQGRFSR
                     TAAKLFLVGIYNNNEKNYTEALALVREGRQEFPRSSFFHFLEILTLENIGDWAAMRQE
                     AESFIRRVESGDFSYRKKYLHRAYFALGNSSLGEKKPAEAVAYYDRVIRDLPSEDRWV
                     TWTYLNRGKAHDLLGRREQALSDYRAVLQRRDVWGMHDQAEDYISRPYAGLPGRDSPI
                     K"
     gene            complement(2985..5129)
                     /locus_tag="P5079_09045"
     CDS             complement(2985..5129)
                     /locus_tag="P5079_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY30166.1"
                     /translation="MSELLIPNYLRAAVVNQRIYPPGSPIVERSVTQILQTLSAPLAE
                     SDRITFTSRQGKLFLKNKEIPDGASLCPYLDEHGLQSLSFLTGVTAAEISQLVILLSR
                     KKLPGGNAAEWLRSQQVTHVQVDKITMVELLEGEVVQKKVDQLFEGLRDFPGMLTSLR
                     ESYDMIDKIPDEAGKAKVQEHIARKLALTEPSLLRDMFENELPKHVEDSGLKTMVLNA
                     MTQDKIHDIFSEIGNWYRQIRAQTNSDFEVVEHLNKLKSFLGKLLNAPASKQVPFAVF
                     EEMLNEGLIDQIPAGVEKKPEEESLASQVDHLLERPGSALLEQPARDQLPFILKKLCD
                     VGLDDMSQRLVEKFVENFSQDVLLLRQMAAHTAKNLMDVLWANRKERLWKIIAERIEK
                     MADSERAGEVYKELADALSSMVHNFILHGRSPEAVRVLALLRRHGAEDNPLMPKRRET
                     ATQTLQQLAASLTDVLTEELLSDDKNRKEAAQDILAHFGENAVPCFIRLIKKSGDLRH
                     RRLAAGVLKYFGESAKSQLAAEFQIGNPTDVLLNILGILEDFLTPDLIPRFENFLHYP
                     DPTIRRRLIHLLARIPSPDASRLLTAFLDDGDESVLVEAVRAVGETRHHGAVEKLCNL
                     LKNGTTRQLEETCLALGHLGNDKALDALLDILEPKSGLFKKRAAVEETVRVRAAWAIA
                     QFKSVPARDALTRLLKDPNLQVQNIARTALAS"
     gene            5335..5550
                     /locus_tag="P5079_09050"
     CDS             5335..5550
                     /locus_tag="P5079_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30167.1"
                     /translation="MKPNERFNADIDGFEILRFNEKNYGLKFRLKGKKGGTGLVRLSK
                     DELALLVFTALHDILDIGVAPRFVKGK"
BASE COUNT         1017 a         1504 c         1940 g         1167 t
ORIGIN      
        1 cggtacgcgc acgatgaccg cggtgttttt ttcgaggcag agggggaaga gtttttcggc
       61 gggcgattga tcgaagatgt tgtagatcac ctgaacgctg tcggcccggc cggaccgcac
      121 cagttccagg gcgctttccg gttcgtggtc gttgatggaa acgccccaga gccgtatctt
      181 ccccgttttt ttcaaggact ccaccgtctc ccaccattcg tcctcccgca tccagctgtc
      241 cgaccagaca tggaattgga tgaggtcgat ccgttccagc gacaggttcc gtagggtttt
      301 gtccagacag gagacgatcc agtctttggg gaagacttga cgcgccaggg tgtcgtggcg
      361 ggcgggccag ccgttgtttt tcggggggat tttcgaggcc acgtagacgt tcgtgggctt
      421 cttttcgcgg aacgcccgtg cgatcaactg ttcgctgtgg ccttttccgt acacgagggc
      481 ggtgtcaaaa aaggtgatgc ccaactccag ggcccggtgg agggctttca gggaggactt
      541 atcgtcgatc gagcggcccc accacgtgcc gccgatgccc caggagccga acccgatttc
      601 ggagacgtcg aacccggctt ttccgaaagg ccgggacttc acgcgtgttc ggacttccag
      661 agccagtaga ggccgaaagc gcccaggcag atctccaaga gaaggacgaa gaccaggggc
      721 aggaggatca agggggggga tttgggatgg aggatcaccc accacgtgcg gtcgcccagg
      781 cgcatggtga aataagtgtc gaagggcgtg gaacgttcca cggcggcgga cggcagcgaa
      841 gtgtagattt cccggccgtt ggtctcgtcg atgatggtga aattgtaatc cggtccggcg
      901 aatttttcca ggaagagggc gttgaagtcg ttgaggcgga agacgccctc gatgaacccg
      961 gcccagcgtt cttcgcggac gatgggttcg tagaggatga cgccctttcc tccttgaacg
     1021 aggtcgatga aatccgtgac ggaggtcttc cggtgagtga tggcccggtg cgccacggga
     1081 agaacgtcga agcggctttt aatgtccagg cggagcgcgt tccggttggc ggcgaggggg
     1141 tggacgtcgt cgatgatgaa atcctggttg atgtggttga tggcggtgaa ggcggggcat
     1201 tttttcagga tgtccgccgc cgatggctgg aagtgcgccg gggcgtccgc cgcgcccgcc
     1261 gcaaacggct gggcgatggt cgcgagccct tggaggtgct ggtcgatgat ctcctgggaa
     1321 ttggtctgca cccaatgcgt gagtttcgcc gagcgcgcgc tgaaactgcg gaggctctct
     1381 tttttgacga accacacgga ggcggccatc agggaggccg tcaacgccag aagccccgcg
     1441 ctgagggaga gaaggcggag ggataccggt tttatatacg gagaaaggtc tttttccaga
     1501 gtggcctcga tcaagccgtc agctccttga acacaaaaac tcccctctcc ggaaacaggg
     1561 gaatcccgga aggcagaggt tgattgttcg gcataaagaa tcctcgcgag gactatttct
     1621 cgggggagag agtccgtcgg tcggcaaacg gtgaggccga gatcgtggga cggaggtgga
     1681 catgaatccc cacgttacga ctccttttgg attctagcat aagccggcgc ccccgggagg
     1741 agaaaatttt caaaacggag acctacggga cggtctattt tatgggggaa tcgcgccccg
     1801 ggaggccggc gtagggccgg gagatgtagt cctcggcctg gtcgtgcatg ccccagacat
     1861 cccggcgctg caggacggcg cggtagtccg acagggcctg ttcccggcgg ccgaggaggt
     1921 cgtgggcttt tccccggttg agataggtcc aggtgaccca gcggtcttcg gaagggagat
     1981 cccggatgac gcggtcgtag taggccacgg cttccgcagg ttttttctcg cccagggaac
     2041 tgtttccgag ggcgaagtag gcgcggtgaa ggtatttttt ccggtaggag aaatccccgc
     2101 tttcgacgcg ccggatgaaa ctttcggcct cctgccgcat ggcggcccag tcgccgatgt
     2161 tttcgagggt gaggatctcc aggaaatgga agaaggagct gcgcggaaac tcctgccggc
     2221 cctcgcggac cagcgccagc gcctcggtgt agttcttttc gttgttgtta taaataccga
     2281 cgaggaaaag tttggcggcg gtgcgggaaa aacgaccctg cgtcatggcc cgccggattt
     2341 cggccagacc ctgttccttg ttgccgccga agaccagttt cgcgaggagc ttcaccacgc
     2401 gggggagtgt ggcggtgtag taatggaaaa tccccacccc caggccggcg tcgtggagtt
     2461 ggggattgat cttcaaggcc ttttcctggg cgttgaacgc ctgtttgccg tggcgatagg
     2521 cccggaacca gtgtccttgg atggcatccc agcggctcat gagtccgagc gcgccgccca
     2581 ggcagaggtg ggcttcgccg cccggatcgc ctcggcggat cttttcgcgg gcgatgacga
     2641 ccgttctttc ggcggcttcg atgaattttt tttccagagc ggggttttgc tcgtcgaatt
     2701 cgttcgtcaa ttcccaccag tgggtggtgg tcagggcgta ttggccgatg ggatcgtccg
     2761 ggaatttttc ggccagcacg gtgaagttct gccgggcggc ttcgtagtcg aggtcgtaga
     2821 gggcccgtag cccgtcgtcg atggggcggg ccatctccgg gtcctgaaag ggggacgccg
     2881 cggccgcccg caggccgggg gggagcgcga gaagcaggag gggcaggagg acggggagga
     2941 ggcggcgggg agtcatcgat cggccgacgg aaagcgggga agggtcagga ggcgagggcc
     3001 gtgcgcgcga tgttttggac ctgcaggttg gggtccttca gaaggcgcgt gagggcgtcc
     3061 cgcgccggga cgcttttgaa ttgggcgatg gcccaggccg cgcggacgcg cacggtctcc
     3121 tcgacggcgg cccgtttctt gaaaaggccg gatttcggtt ccagaatgtc cagaagcgcg
     3181 tcaagggctt tgtcgttgcc gagatgcccc agggccaggc aggtctcttc caattggcgg
     3241 gtggtgccgt tcttcagaag attgcagagc ttctccacgg ccccgtggtg tcgcgtttcc
     3301 cccacggcgc ggacggcctc cacgaggacg ctctcgtcgc cgtcgtcgag aaaggccgtc
     3361 aggaggcgcg aggcgtccgg agacgggatc cttgccagaa ggtggatcag gcggcggcgg
     3421 atggtggggt ccggataatg caggaaattc tcgaagcggg ggatgaggtc cggcgtgagg
     3481 aagtcctcca aaatgccgag gatgttcagg aggacgtcgg tggggttgcc gatttggaat
     3541 tccgcggcca gctggctctt ggccgattcc ccgaaatatt tgagcacgcc ggcggccaac
     3601 cggcgatggc gcaggtcccc cgattttttg atgagacgga tgaaacaggg gacggcgttc
     3661 tcgccgaaat gggccaggat gtcctgcgcc gcctccttcc ggtttttgtc gtcggaaaga
     3721 agctcttcgg tgagcacgtc cgtcaggctc gcggcgagct gttgcagcgt ctgcgtcgcg
     3781 gtctcccggc gtttcggcat gagggggttg tcttcggccc cgtgccggcg gagaagcgcg
     3841 agcacccgga ccgcttccgg ggagcggccg tgcagaatga aattgtggac catggaagaa
     3901 agggcgtcgg ccagctcttt gtagacctcg ccggcgcgct cgctgtccgc catcttttcg
     3961 atgcgttcgg cgatgatctt ccaaaggcgt tccttgcggt tggcccacag gacgtccatg
     4021 aggtttttgg ccgtgtgggc cgccatctgc cgcaggagga gcacgtcctg ggagaaattc
     4081 tcaacgaatt tttccacgag acgctgggac atgtcgtcca gccccacgtc gcagagtttc
     4141 ttgaggataa agggaagttg gtcccgcgcc ggttgttcca acagggcgga gccggggcgc
     4201 tccagcaaat ggtccacctg ggaggcgagg gattcctctt cgggtttttt ctccaccccc
     4261 gcggggatct ggtcgatcaa tccctcgttc agcatctctt cgaagaccgc gaagggcacc
     4321 tgcttgctgg ccggcgcgtt caggagtttt ccgaggaagg acttgagctt gttcaggtgc
     4381 tccaccactt cgaaatccga gttggtctgc gcgcgtatct ggcggtacca gttgccgatc
     4441 tcgctgaaaa tatcgtgtat tttgtcctgc gtcatggcgt tgaggaccat ggttttgagt
     4501 ccggaatctt ccacgtgttt cgggagctcg ttttcgaaca tgtcgcgcag gagggagggc
     4561 tccgtgaggg ccagtttccg ggcgatgtgt tcctggacct tggcttttcc cgcctcgtcg
     4621 ggtattttgt cgatcatgtc gtaggattcc cgaagggaag tcaacatgcc ggggaagtct
     4681 cggaggcctt cgaagagctg gtccaccttt ttctggacga cctctccctc caggagttcc
     4741 accatggtga tcttgtccac ctgcacgtgc gtcacctgct gggaacggag ccattcggcg
     4801 gcgttaccgc cggggagttt cttccggctg aggaggatga cgagctggga gatctccgca
     4861 gccgtgacgc ccgtcaggaa actcaggctc tgaaggccgt gttcgtcgag atagggacag
     4921 agggaggccc cgtcggggat ttccttgttc ttcaggaaaa gcttgccctg ccgcgaggtg
     4981 aaggtgatcc gatcgctttc cgccaacggg gcggacaggg tttggaggat ctgcgtcacg
     5041 gagcgttcca cgatggggct gccggggggg tagatgcgct gattgacgac ggcggcccgc
     5101 aggtagttgg ggatgagcag ttcactcatg gagacagccc tttccggcga ttgaagatgg
     5161 gacaacctgt gccactcccg cggtccgcgc gagctcgtcg ggcggataaa aaaagattat
     5221 acataaattc ggcgcgccgt ccgcgcgcgc ggattgaatt tgaacggcgt ttctgataac
     5281 ctacgccaga cgccctgtcc gtcgccggcc ggggaacgat cgcaggaggc gcccatgaaa
     5341 ccgaacgaac gattcaacgc ggacatcgac gggtttgaga tactgaggtt caacgagaag
     5401 aactatggtt tgaagttccg gttgaaggga aagaaggggg gcaccgggct ggtgcgtctt
     5461 tccaaggacg aactcgcgtt gctggtgttc accgcgcttc acgacatttt ggatatcggg
     5521 gtggcgcccc ggttcgttaa agggaaataa aaaagcggcc ccgcgttcgg cgggaccgct
     5581 tttttaaacg tttttgagcg gcgcgaaaaa gaaagttacg gcgccggg
//
LOCUS       DAOTFD010000172         5596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10367_length_5596_cov_3.547374, whole genome shotgun sequence.
ACCESSION   DAOTFD010000172 DAOTFD010000000
VERSION     DAOTFD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10367_length_5596_cov_3.547374"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4379)
                     /locus_tag="P5079_09055"
     CDS             complement(<1..4379)
                     /locus_tag="P5079_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY30168.1"
                     /translation="MRINEVAPRQAVAQGGDWIELYVATTTLTDMQGWTISFLNGGPT
                     LKVSSITLTALTTAQAYVVIHLSTSTEATSPADENDGTGDTNLNGYWDIYIGSDTVNN
                     ADGLQGTDNVLVLRDNTGLVIDGMVYSNGNATWSGNTAANNLLQLLVSTGQWSGPASV
                     SAVAAERTDQCGVTYTGTTAGVGLHRDQFSTDNDNVGFRKSEWGVRLGLSTGAANADP
                     TDYYPPAAITDLDVTAQSASSLTLQWTVPSAGASTDTLKQYYVRYTSATLLGTVTDFF
                     AASNFTSNVTDFTAGNSTGIVVNGLLPATTYWFAVVAEDDGDAAAGNFKWGGLPTNLP
                     VSGLTSPDTEAPAAITDFVAVAGSTMCGAVNLTWTAPSDPPTGGAATKYIVRFATTSA
                     AEVGWSSSTWWAGATDVPLEPTPAVPGTAQAWTLSGLPSGVTCYFHIRSQDAVNISSS
                     QTNAEQYVYIQSHLLISQIKLGTAELDFVELYNPSTNTINLQGLPLYYHRRTNTGTDT
                     NVALTYVNTSIAPHSFFVFAPDSTVTGGVAPDATNTVDMYSGNSSIYISLSATELVNV
                     IDVLGTGTANSDPYIDYGDANAPTRFTPNPATGAGLIRRPAGGLGHATDTNKWTGDFM
                     STATPVARNSLSTPEPDPTAPAAVTNLFSAKGLSGGEINLYWTAPGDDGGSGAITGGQ
                     YDLRYATFSVSGGDTASWWGHPSVTSVLFSTTTSPGATETRTVVGLTTSTIYYFAIKT
                     KDESGNWSAIDDGAAVSQTNTPPKEASGGSYSALGANAVMAVDNFDDGDLTNLLGKGC
                     AIFEVSGATTTLTVTSSPAADMFGRRGYVLKVHYEEDGASWAGFWNGLQGIDLTGYRY
                     LSFWIRGEAGGEMVKVGLKNNGGQENPKPYIYEFLPGGITTSWRKVVIPFTAFGTLTS
                     FSNMDSIMFVIDEASSNGTVYIDDIVLGKNGAPGWIDNFSDGNTTTAFGAGNDVFAGG
                     AGTSMSRTIDGDGYRDSAVKVAWGGVTPFNNKPGWWISAQNNNASSPHQGLDARGTTG
                     LSFYVKGSPAGASFYVGVKDLSQAGWAIEKKVLLTDYLPGGVTGTYQEVRIPVTALAD
                     PDVHVEGLESLSLEPLTADATVYLDEFVFIDTAPPSAPFNLQDDGNAVANSHVFGSTN
                     ILTLQAARGDTDKSLESVRVEYSTDSGSTWVVAGRDYDVGNDTYTVTWYTTGLSTTAS
                     YDVRGVAEDVNGSTGVMTAFTGCSVVQDDTTPPAAVTNLFSAKGLNSGETYLYWTAPG
                     DDGTAGAITGGEYEIRYAAGEVGVGLDTTTWWNNAAGTSILFSTTTAPGSTESRTVVG
                     LTTSTTYYFAIKTKDETGNWSAIDALAASNSQNWSRPREGTAGSYPALGANGVLVLDQ
                     FDDGDTTNALGWAWGTYKESGGEVTISPWNDLGETFGRGGYSLKMNYTHNGSPSNKLL
                     VYGALNAADLSAYNYVSFWVMGST"
     gene            complement(4819..>5596)
                     /locus_tag="P5079_09060"
     CDS             complement(4819..>5596)
                     /locus_tag="P5079_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30169.1"
                     /translation="QKIFAQAALRDMLEPATDAEFAARSRGRMEALLSELTAQGMESG
                     VGEAEMLADLIANAGVEAYNGMMARPLRPAPAGKETKFRDEVRADVKTTFGVALEMTA
                     NMVKQTEGWSGQEQTGFSQIHRLAAHIDELSGAVVEVEYDERMQGLSEAMLLRELAKN
                     AGLDEAAVNELLGHLGKKIFVRVRKAGEKIDVAQVRGLLNVQNLDIFAMDAAHWVVPQ
                     EWLKSGVVRLLVMLAGDVVTNATQKLHEDIQRVQFIRIQA"
BASE COUNT         1064 a         1863 c         1550 g         1119 t
ORIGIN      
        1 gtcgagccca tcacccaaaa cgacacatag ttataggcgc tcaaatcggc cgcatttaac
       61 gccccgtaca ccaacaattt gttgctgggc gacccgttgt gggtataatt catcttcaaa
      121 ctgtatccgc cgcgcccaaa tgtctcgccc agatcattcc acggcgagat ggtcacttcg
      181 ccgcccgact ccttataggt cccccaagcc caccccaaag cgttcgtggt gtcaccgtca
      241 tcaaactgat ccaaaaccaa cacgccgttg gcccccaggg ccggataact ccccgcggtt
      301 ccttccctcg gacgggacca attttggctg ttgctcgccg ccaacgcgtc gatggcggac
      361 cagtttcccg tctcgtcctt ggttttaatg gcgaagtaat acgtggtgga agtcgtcaac
      421 cccaccacgg tgcggctctc cgtggacccc ggcgcggtcg tggtggagaa caggatggac
      481 gtccccgcgg cgttgttcca ccaggtggtg gtgtccaacc ccacacccac ctcgccggcc
      541 gcatagcgga tctcatactc tccgcccgtg atcgccccgg ccgtcccgtc gtcccccggc
      601 gccgtccaat acagataggt ctccccgctg ttcaaaccct tcgccgaaaa caaattcgtc
      661 acagcggccg gcggggtggt gtcgtcctgc accaccgaac atcccgtgaa cgccgtcatc
      721 acgcccgtgc tgccgttcac gtcttcggcc acgccgcgca catcgtagct ggcggtggtg
      781 ctcaaccccg tggtatacca cgtcaccgtg taggtgtcgt tgcccacatc gtagtcccgg
      841 ccggcgacga cccaagtgga gccgctgtcg gtggaatact ccacccgcac gctctccaga
      901 cttttgtccg tgtcccccct cgccgcctgc agggtcagga tgttggtcga accgaacaca
      961 tggctgttcg ccaccgcatt gccgtcgtcc tgcagattga acggcgccga cggaggagcc
     1021 gtgtcgatga acacgaactc gtccaaatac accgtcgcat ccgccgtcag cggctccagg
     1081 ctgagactct cgagcccctc cacatgcacg tccgggtccg ccaacgcggt caccgggata
     1141 cgcacttctt gataagtccc cgtcacgccg cccggcaggt aatccgtcag caacaccttc
     1201 ttttcgatcg cccatcccgc ctggctcaaa tctttcacgc ccacataaaa actggccccc
     1261 gccggcgatc ccttcacgta aaaactcaac cccgtcgttc cgcgcgcgtc cagcccctga
     1321 tggggactcg acgcgttgtt attttgcgct gaaatccacc atccgggttt attgttgaac
     1381 ggcgtcaccc cgccccaagc caccttgacg gcgctgtcgc gatatccgtc cccgtctatc
     1441 gtcctcgaca tcgacgtccc cgcgccgccg gcgaacacgt cgttgccggc gccgaacgcc
     1501 gtcgtggtat tgccgtcact gaaattatcg atccatcccg gcgccccgtt ctttccaaga
     1561 acgatgtcgt cgatatagac cgtcccgttg ctcgaggcct cgtctatcac gaacatgatg
     1621 ctgtccatgt tcgaaaaact cgtcagcgtc ccgaacgccg taaacgggat caccaccttc
     1681 cgccaactcg tcgtgatacc gcccggcaag aattcataga tgtagggttt gggattctcc
     1741 tggcccccgt tattcttcaa ccccaccttc accatctcgc cgcccgcctc gcctcgaatc
     1801 cagaacgata aataccggta accggtcaga tcgatcccct gcaaaccgtt ccagaacccg
     1861 gcccaggaag ccccgtcttc ctcgtaatgc accttcaaga catagcccct tctgccgaac
     1921 atgtcggccg ccgggctgct cgtcaccgtg agcgtcgtcg tcgcccccga cacctcgaaa
     1981 atcgcgcaac ctttcccgag cagattcgtc aggtccccgt cgtcgaaatt atcaaccgcc
     2041 atcaccgcgt tcgcgccaag ggccgaatag ctcccgccgc tcgcctcttt cggcggagtg
     2101 ttcgtctgcg agaccgcggc cccgtcatcg atcgccgacc agttccccga ctcgtccttc
     2161 gtcttgatcg caaaataata aatcgtcgag gtcgtcagcc ccaccaccgt ccgagtctcc
     2221 gtcgcccccg gcgacgtcgt cgtcgagaac aacacggacg tcacggacgg atggccccac
     2281 caggacgccg tgtcgccgcc ggaaacggaa aacgtcgcgt agcggaggtc atactggccg
     2341 cccgtgatcg ctccgctccc cccgtcgtcc cccggcgccg tccaatacag gttgatctcc
     2401 ccgcccgaca gccccttggc ggaaaaaagg ttggtgacgg cggcgggggc ggtggggtcg
     2461 ggctccggtg tcgacagcga attgcgggcc accggcgtgg cggtgctcat aaaatccccg
     2521 gtccatttat tcgtgtcggt tgcgtgaccc agcccgcccg ccgggcggcg gatcaatccg
     2581 gccccggtgg cggggttggg cgtaaagcgg gtcggtgcgt tggcgtcgcc gtaatcgata
     2641 tacggatcgc tgttggccgt gcccgtcccc aacacatcga tgacgttcac caattccgtg
     2701 gcgctcaaac tgatgtaaat ggagctgttc cccgaataca tatccacggt gttggtggcg
     2761 tcgggagcta ccccgccggt caccgtggaa tccggggcga acacgaaaaa agaatgtgga
     2821 gcgatggaag tgttgacata tgtcaacgca acgttggtgt ccgtccctgt attggttctt
     2881 cggtgataat agagcggaag cccttgcaaa ttgatggtgt tggtcgaagg gttatagagc
     2941 tcaacaaaat ccaattccgc agtccccaat tttatctggc tgatgaggag gtgcgactga
     3001 atgtagacgt attgttcggc gttggtctgc gacgaggaga tattgacagc gtcctgactg
     3061 cggatgtgga aataacaggt caccccggaa ggcagcccag acaacgtcca cgcttgggcg
     3121 gttccgggga ccgcgggcgt gggctccaaa ggcacatccg tggcccccgc ccaccacgtg
     3181 gatgaagacc accccacctc cgccgcggag gtggtggcaa accgaacgat gtattttgtg
     3241 gcggcgccgc cggtgggcgg atcggacggg gccgtccacg tgagattgac ggccccgcac
     3301 atggtgctgc cggccaccgc cacgaaatcg gtgatggcgg cgggggcctc cgtgtcgggg
     3361 ctcgtcaatc cggacaccgg cagattggtg ggcaaaccgc cccacttaaa attgccggcg
     3421 gcggcatcgc cgtcgtcttc cgccaccacc gcaaaccagt aggtggtggc cggcaacaac
     3481 ccgttgacga caatcccggt ggaattcccc gctgtaaaat cggtgacgtt gctggtgaaa
     3541 ttgctggcgg caaagaaatc cgtgaccgtg ccgaggaggg tggctgacgt gtagcggaca
     3601 taatactgtt tgagagtgtc ggtggatgcc ccggcggacg gcacggtcca ctgaagggtc
     3661 agagacgagg cgctctgtgc ggtcacgtcc agatccgtga tggccgccgg cggataataa
     3721 tccgtcggat cggcgttcgc cgcgccggtg ctgagaccga gccgaacccc ccactcgctt
     3781 ttacggaaac ccacattgtc gttgtccgtc gaaaactggt cgcggtgcaa cccgaccccc
     3841 gccgtagtcc cggtatacgt cacgccgcat tgatccgttc gttccgccgc gacggcggaa
     3901 acggacgccg ggccgctcca ctggccagtg cttacaagaa gctgaagcaa gttatttgcg
     3961 gcggtattcc ccgaccatgt ggcattgccg ttggaataga ccatgccgtc gatcaccaac
     4021 ccggtattgt cacgcaacac cagaacgttg tccgtcccct gaaggccatc ggcgttgttc
     4081 acggtgtcgc tgccgatgta gatgtcccaa tacccgttga ggttggtgtc accggtacca
     4141 tcgttctcat ccgccggact ggtggcctcc gtcgaggtgc tgagatggat aaccacgtag
     4201 gcctgcgcgg tggtcagagc ggtgagcgtg atgctggaaa ccttcaaggt cggtccgcca
     4261 ttaaggaaac tgatggtcca gccctgcatg tccgtgagag tggtggtggc gacataaagt
     4321 tcgatccagt cgcccccctg cgcaacggcc tgccggggcg ccacctcgtt gatgcgcacg
     4381 tcggacgcag aaaaggcctc ccggggaaga gcgataaagc tcaaacccaa gaggcctaaa
     4441 aaggcgatac ctcggcgtgc tcggcggaat aacattttaa gtgctgaaaa ctccggcgga
     4501 tggccgccgg cggttttcaa gcgcccattc ggagcgtggg ggccgttttc tctcctttat
     4561 tcaagcgctt caaatacctt ttttaaaaaa tccccgcgga gattcccaat tattccggca
     4621 acagacgttt ctattttacc acttttccat aaaaaaagtc ggtttttttc cacccgggcg
     4681 gcgtagttac aatattcctt aatataagat aaaacccctc cccttcaaaa caatctgaag
     4741 gggaggggcg gatcgggtct tgcggggaga gaggagacgc aagacccgat tcaaatctgt
     4801 gttttatcca cgtcctcctt acgcctggat gcggatgaac tgcacccgct gaatgtcctc
     4861 gtggagtttc tgcgtggcgt tggtgaccac gtcgccggcc agcatcacca acaaccgcac
     4921 cacgccgctc ttgagccatt cctgcggcac cacccagtgg gcggcgtcca tggcaaaaat
     4981 gtcgaggttc tgcacgttca gaagaccgcg cacctgggcg acgtcgatct tctcgcccgc
     5041 cttccgcacc cgcacgaaaa ttttcttgcc caggtgtccc agaagttcgt tgacggccgc
     5101 ctcatccaat ccggcgttct tggccagttc ccgcaaaagc atcgcttccg acaggccctg
     5161 catccgctcg tcgtattcca cctccacgac ggcgccggaa agctcgtcga tatgcgccgc
     5221 cagacggtgg atctggctga aacccgtctg ttcctggccg ctccatccct cggtctgttt
     5281 caccatgttg gcggtcatct ccagggccac gccgaacgtc gtcttcacgt ccgcccggac
     5341 ctcgtcgcgg aacttcgttt ctttcccggc gggcgcgggg cgcagcggtc tcgccatcat
     5401 gccgttgtag gcctccacgc ccgcgttggc gatcaagtcc gccaacatct cggcctcgcc
     5461 cacaccggac tccatgccct gcgccgtcaa ttccgacaac agcgcctcca tccggccgcg
     5521 ggaccgcgcc gcgaactcgg cgtccgtggc cggttccagc atgtcccgca aggccgcctg
     5581 cgcaaagatc ttctgc
//
LOCUS       DAOTFD010000173         5561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10479_length_5561_cov_4.632946, whole genome shotgun sequence.
ACCESSION   DAOTFD010000173 DAOTFD010000000
VERSION     DAOTFD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10479_length_5561_cov_4.632946"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="lon"
                     /locus_tag="P5079_09065"
     CDS             <1..1252
                     /gene="lon"
                     /locus_tag="P5079_09065"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY30170.1"
                     /translation="SLARSIARALGRNFVQMSLGGVRDEAEIRGHRRTYIGSLPGRIL
                     QSMRKAKSKNPVFLMDEVDKMGSDWRGDPAAALLEVLDPEQNHSFVDHYLDVAFDLSE
                     VMFITTANSLYSIPPSLQDRLEVIRFPGYTTEEKVAIAMKFLVPKQMRDHGLKPDQFR
                     VEESAVKAIIRDYTQEAGVRNLEREIAHLCRKAARRMVGDKKKSVRVGAADLRAFLGV
                     PKFLKERTSPNAIGVATGLAWTDHGGETLAVEVTVMPGKGKLILTGQLGEVMKESAQA
                     ALSYVRSLSRRLHIKKNAALKDHDMHIHIPEGATPKDGPSAGIALATAVASALTGRAV
                     RKDLAMTGEITLRGRVLPIGGFKEKVLAAYREGLQTVLYPQGNLKELEEIPRDIQKKL
                     KLVPVSTMDDVLAHALERHRGKIS"
     gene            1317..2453
                     /locus_tag="P5079_09070"
     CDS             1317..2453
                     /locus_tag="P5079_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRY30171.1"
                     /translation="MKKYYLITPGPTPVPPTVAAQSALPVLHHRTHEFGKLFEQVMEG
                     LRTVFQTKRDVMLITGSGTGAMESAVANLLSPGDKAIVASCGAFGNRWEKICVAFGAK
                     VVMLKEEWGQAVDPGKVRQALKENPDAKAVFFTHTETSAGTVNDVKAIAAVVAETAAV
                     SVVDAISGLAGQELRMDDWKLDVVVSGSQKGLMTAPGLAFAAVSEKGWKLVESSKNAR
                     FYNDYRLIKKSIPNKETPWTPAVSLVASLHEAIRALMEEGMENVWKRHLRLAKACRAG
                     MQALNLKLLSAAPCSVLTSAHLPESVSGEKLVDWLRDEHGVSIAGGQEHLKGKIVRLA
                     HMGYMDHFDLTIGLTALEWGLVKFGHKVDAGKAVKTAQETFLAG"
     gene            2473..4062
                     /gene="serA"
                     /locus_tag="P5079_09075"
     CDS             2473..4062
                     /gene="serA"
                     /locus_tag="P5079_09075"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRY30172.1"
                     /translation="MNMPIKILVSDPVSKEGLEPLLKNPMFSVEIKTGMTPQELLAHV
                     AEAQALLVRSETKVTAEVFAAAKQLRFVGRAGTGVDNIDLKAATAAGVVVANVPGGNT
                     ISAAEHALALMFSLARNVPAADASIRAGKWERSKFVGTELTGKTLGVLGLGRIGKEVA
                     TRAVGLNMRVLAYDPVGDDNWCRLARVTPVSFDDLLKQSDFVTVHVPLNPNTKGLLNR
                     DSLAKAKPGLRLINCARGGIVDEAALLEAIEKGQVAGAALDVFEKEPPKDNPLLQRPE
                     VIVTPHLGASTEEAQVKVAYELSLALIDFFEKGYAKHAVNLPSVDVAGQTQLLSYVPL
                     AYRLGLFLGQMVEGEIKSVSLAYSGELGRCQSALFTVTALAGILRSSDAKASPVNAAA
                     KAAEKGIQVKEEAKTEAKDYASLLEIEVATAQGAQRLAGTVYGRGDFRLVRINDLPID
                     IQPEGHLLVMSNDDKPGVVGHTGTVLAEGGINIAGLEVGRNKPGGVAVSVWSVDGAVS
                     ASVLAKVKKHPAVLSVRMVEL"
     gene            4107..4871
                     /locus_tag="P5079_09080"
     CDS             4107..4871
                     /locus_tag="P5079_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY30173.1"
                     /translation="MNPSSALRLENLTKVYRRSHLGRVKLSRGVENISVDIRPEEVFG
                     LLGLNGSGKTTTIKLILGLLFPTTGKVEVLGHPAGGMEAKRSIGYLPEVPYFYKYLSA
                     REVLFFYGRLSGVDAASLPRRVEEVLSTVRMAAHADRPMREYSKGMLQRIGLAQAMLH
                     DPRLLVFDEPVTGLDPLGLREMRELLLSLNKAGKTVFFSSHSISEVEKVCHRVGILVN
                     GRLARVVEQGEWAGKEGHLEEIFVETVSSGGAAEVF"
     gene            4871..>5561
                     /locus_tag="P5079_09085"
     CDS             4871..>5561
                     /locus_tag="P5079_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY30174.1"
                     /translation="MRSVLTIISYTFLQQFRNRLYLVVVFFGCVLLAVSLLFGAVAAD
                     QEVRVILDLGLAMTEFFGLLTAIFGAVTLVLEEMESRTIYLILTRPLPRGYYILGRFL
                     GLLSAVAASMLLMGVLHLLLLGLKGWEWDFRYFVCYPPMFFKVMVISSLALFFSLFSS
                     SAVSSVVFTFFFWVLGHFGPELKFLAQKNQNLPMTVGTKIFLFITPDLQMLNYRDFFH
                     VPGLSAAHVLPG"
BASE COUNT         1005 a         1748 c         1774 g         1034 t
ORIGIN      
        1 ctcgctggcc cgttccatcg cccgcgccct gggccggaat ttcgtgcaga tgtccctcgg
       61 cggcgtgcgg gacgaggccg agatccgcgg ccatcgccgc acctacatcg ggtccctgcc
      121 cggccgcatc ctccaatcca tgcgcaaggc caagtccaag aacccggttt tcctgatgga
      181 cgaggtcgac aaaatggggt ccgattggcg cggcgatccg gcggccgcgc tcctcgaagt
      241 gctggacccg gaacagaacc attccttcgt ggatcattac ctggacgtgg ccttcgattt
      301 gtcagaggtg atgttcatca ccacggccaa ttcgctgtat tccattccgc ccagccttca
      361 ggaccggctc gaggtcatcc gcttccccgg ctacaccacc gaggaaaaag tggccatcgc
      421 catgaagttc ctcgtcccca aacagatgcg ggaccacggc ctgaagcccg accagttccg
      481 cgtggaggaa tccgctgtca aggccatcat ccgggattac acccaggagg cgggtgtgcg
      541 gaacctggaa cgggagatcg cccatctctg ccgcaaggcg gcccggcgga tggtggggga
      601 caagaagaag tccgtgcgcg tgggggccgc ggacctgcgc gctttcttgg gcgtgccgaa
      661 atttttgaag gagaggacgt cccccaacgc catcggcgtg gccacgggac tcgcctggac
      721 ggaccacggc ggcgagacgc tggccgtgga ggtgacggtg atgccgggca aggggaaact
      781 catcctcacc ggccagttgg gcgaggtgat gaaggagtcg gcccaggcgg cgctgtctta
      841 cgtgcgctcg ctgtcgcgcc ggctgcacat caagaagaac gccgccctca aggaccacga
      901 catgcacatc cacatccccg aaggggccac gcccaaggac ggcccttccg ccggcatcgc
      961 cctggccacc gcggtggcct ccgcgttgac ggggcgcgcg gtgcggaaag acctggcgat
     1021 gaccggcgag atcacgctgc gcggccgcgt gctgcccatc ggcggattca aggagaaggt
     1081 gctggccgcc taccgggaag ggctgcagac cgttttgtat ccccagggaa acctcaagga
     1141 gttggaggag attccacggg acatccaaaa aaagttgaag ctggtgcccg tgagcacaat
     1201 ggatgacgtg ctcgcccacg cgttggagag acatcgcggc aaaatcagtt agccggcaga
     1261 accgctccct tccgcacaac accaattcac gaaaaattaa aggagatcca cacgccatga
     1321 aaaaatatta tctgatcacc cccggtccca cccccgtccc tccgacggtg gcggcacaaa
     1381 gcgcccttcc ggtcctgcac caccgcacgc acgagttcgg caaactgttc gaacaggtga
     1441 tggaggggtt gagaaccgtt tttcagacaa agcgggacgt catgctgatt acgggctccg
     1501 gcaccggggc catggaatcc gccgtggcca acctgttgtc tccgggagac aaggccatcg
     1561 tggccagctg cggcgccttc gggaaccgct gggagaaaat ctgcgtcgct ttcggcgcca
     1621 aggtcgtgat gctcaaggag gaatggggcc aggccgtgga tccgggaaaa gtgcgtcagg
     1681 cgctgaaaga gaatccggac gccaaagccg tgttcttcac ccacaccgag acgtccgccg
     1741 gaaccgtcaa cgacgtgaag gccatcgccg ccgtggtggc ggagacggcc gcggtgagcg
     1801 tggtggatgc catctccggt ttggccgggc aggaactgcg catggacgat tggaagctgg
     1861 acgtggtggt gtccggttcc cagaaggggc tcatgaccgc ccccggcctg gcgttcgccg
     1921 ccgtttcgga aaaagggtgg aaattggtcg agtcgagcaa gaacgcgcgt ttctacaacg
     1981 attaccggct catcaagaaa tccatcccca acaaagagac gccctggacc ccggcggtgt
     2041 cgctggtggc ctcccttcac gaagccatcc gcgccctgat ggaagagggg atggaaaacg
     2101 tttggaaacg gcacctgcgg ttggccaagg cctgccgcgc cggcatgcag gcgctgaacc
     2161 tgaaactcct ttccgcggcc ccctgttccg ttctgacgtc ggcccatctt ccggaaagcg
     2221 tgtcggggga gaaactggtg gactggctgc gcgacgagca cggggtgtcc atcgccggcg
     2281 ggcaggagca cctgaaaggg aagatcgtcc gcttggcgca catgggctac atggatcatt
     2341 tcgatctcac catcgggttg acggcgttgg aatggggact cgtcaagttc ggccataagg
     2401 tggacgccgg caaggcggtg aagacggccc aggaaacctt cctggctggc tgagcggggt
     2461 ccgcaaggag acatgaacat gccgatcaaa atattggtgt ccgatccggt gtccaaggaa
     2521 gggctggagc cgctcctgaa gaacccgatg ttctccgtcg aaataaagac ggggatgacg
     2581 ccgcaggaac tgctggccca tgtggcggag gctcaggcgc ttctggtgcg ttccgaaacg
     2641 aaggtgaccg cggaggtgtt cgccgcggcc aaacagttgc gtttcgtggg ccgggcgggc
     2701 acgggcgtgg acaacatcga cctgaaggcg gccacggccg ccggggtcgt ggtggccaac
     2761 gtgccgggcg gcaataccat ttcggcggcg gaacacgcgc tggcgctgat gttctccctg
     2821 gcgcgcaacg tgccggcggc cgacgcctcc atccgcgccg gaaaatggga acgttccaaa
     2881 ttcgtcggca cggagttgac ggggaaaacc ctcggcgtgc tggggctggg gcggatcggc
     2941 aaggaggtcg ccacccgcgc ggtgggcttg aacatgcgcg tgctcgctta cgaccccgtc
     3001 ggggacgaca attggtgccg gctcgcccgc gtgacgccgg tttccttcga cgatttgctc
     3061 aagcagagcg acttcgtcac cgtccatgtg cctttgaacc ccaacaccaa ggggttgctc
     3121 aaccgcgact cgctcgccaa ggcgaagccc ggcctgcgtc tgatcaactg cgcccgcggc
     3181 ggcatcgtgg acgaggccgc gctcctggag gcgatcgaaa aggggcaggt ggcgggggcc
     3241 gccctcgatg tcttcgagaa ggaaccgccc aaggacaacc cgctccttca gcgtcccgag
     3301 gtcatcgtca ccccgcacct gggcgcttcc acggaagagg cccaggtcaa ggtcgcctat
     3361 gaactttccc tggcgctcat cgacttcttc gaaaagggct acgccaaaca cgccgtcaat
     3421 ctgccgtcgg tggacgtggc gggtcagaca cagttgctgt cctacgtgcc cctggcctac
     3481 cgcctcggac tcttcctcgg ccagatggtg gagggggaaa taaaatccgt ctcgctcgcc
     3541 tattccggcg aactgggccg gtgccaatcg gcccttttca cggtgaccgc cctggccggc
     3601 atcctccgtt cgtcggacgc gaaggcgtcc ccggtcaacg cggccgccaa ggccgccgaa
     3661 aaagggatcc aggtgaagga ggaggcgaag acggaagcca aggactatgc ctcgctgctg
     3721 gaaatcgaag tggccacggc gcagggggcc cagcgtctcg ccggaacggt ctacggccgc
     3781 ggtgatttcc gcctcgtccg catcaacgac ctgcccatcg acatccaacc cgagggccat
     3841 ctcctggtca tgagcaacga cgacaagccc ggcgtggtgg gccacaccgg caccgtcctg
     3901 gcggagggag gcatcaacat cgccggtctg gaagtcggcc gcaacaagcc cgggggcgtc
     3961 gccgtttccg tctggagcgt ggacggcgcc gtttctgcgt ccgtcctggc caaggtgaaa
     4021 aaacatcccg cggttctgtc cgttcggatg gtggagttgt aaggggaaaa tcgtccggcc
     4081 gcgcgccggt tttgccgagt tcgcccatga atccgagttc cgcgcttcgt ctggagaatc
     4141 tcaccaaagt ttaccggcgg tcccatctgg ggcgcgtcaa actttcccgc ggggtggaga
     4201 atatctccgt ggatatccgc ccggaggagg tcttcggcct tttggggctg aacggttccg
     4261 ggaagaccac caccatcaag ctgatcctcg gtctcctttt ccctacgacg gggaaggtcg
     4321 aggtcctcgg ccacccggcc ggcggcatgg aagcgaagag aagcatcggg tacctgccgg
     4381 aagtccccta cttctacaaa tacctctcgg cgcgcgaagt cctttttttc tacggccgtc
     4441 tttcgggcgt ggacgccgcc tctctccccc gccgggtgga agaggtcttg tccacggtgc
     4501 gcatggccgc ccatgccgac cggcccatgc gggaatattc caaggggatg ttgcagcgga
     4561 tcgggctggc ccaggccatg ctgcacgacc cgcgcctcct tgtgttcgac gagcccgtca
     4621 ccggtctcga tccgctcggc ctgcgggaga tgcgggaact cctcctctcc ctcaacaagg
     4681 ccggcaagac cgtgttcttt tcctcgcaca gcatctccga ggtggaaaaa gtctgtcatc
     4741 gcgtgggcat tctggtgaac ggccgcctgg cccgcgtggt ggaacagggc gagtgggccg
     4801 gcaaggaagg gcatctcgag gagatcttcg tcgagacggt gtcgtcgggc ggcgccgcgg
     4861 aggttttctg atgcgttcgg tcctgacgat catctcttac actttcctcc agcagttccg
     4921 aaaccggctc tatctggtgg tggtcttttt cggctgcgtc ctcctggcgg tttctctttt
     4981 gttcggcgcg gtggcggccg accaggaggt ccgcgtcatc ctcgacctgg gcctggccat
     5041 gacggaattc ttcggtttgc tgacggcgat cttcggcgcg gtcacgctcg tcctggagga
     5101 gatggaatcg cgcaccatct acctgatcct gacccgcccc ctgccgcgcg gttattacat
     5161 cctcggccgg tttctcggtc tgctctcggc cgtggccgcc agcatgctcc tcatgggggt
     5221 tctccatctt ctccttctcg ggctcaaggg ttgggagtgg gacttccgct acttcgtttg
     5281 ctatcctccg atgtttttca aggtgatggt gatctcgtcc ttggcgctgt tcttctccct
     5341 gttctccagc tcggccgtct cgtcggtggt gttcacgttc ttcttctggg tgctcggtca
     5401 cttcggtccg gaattgaagt ttttggccca gaagaatcag aacctcccca tgaccgtcgg
     5461 cacgaaaatt ttcctcttca tcacccccga cctgcaaatg ctcaactacc gtgatttctt
     5521 ccatgtcccc ggcctttccg cggcccacgt gctgccgggc c
//
LOCUS       DAOTFD010000174         5553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10499_length_5553_cov_4.569116, whole genome shotgun sequence.
ACCESSION   DAOTFD010000174 DAOTFD010000000
VERSION     DAOTFD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_5553_cov_4.569116"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5553)
                     /locus_tag="P5079_09090"
     CDS             complement(<1..>5553)
                     /locus_tag="P5079_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY30175.1"
                     /translation="KLLSNTFYQQLLAQANDPALSEEVRRQATTAMGVLSSFSTQGKI
                     EGDQYKIDLSGRQRESMDLNEFLLLQDFILNLPSFQRLVDPRAADIFKGKNALELFEI
                     SGDPDADPFLRSEAQRIIGLAAFMAGMSTSVVDSDGDGVPDDVVIRLVQDGEARTFAL
                     SEDTRVTEMLMKDFMDAFATVLNLEEFQRLLNEGGDFTFTDRNGHKIQVPANAILAST
                     FFGNYGAITDQTLFDLFGVNRLRVDRDGDGVLDDTPLTRFDLLTLNDPRVQAVNSIVV
                     FLSLNRAMLEKETGLTINQIVQKVAQIQPLFREYVQTTAFDRDVKLTEGDFMFLLYLL
                     REAQARHTTLEEMLVEPRFRAEMTVRYQLFDSETMTQKNALRWGNKQAEILYEVEHAQ
                     QSLKLVEHFYDIRTAQENIALATAHLQELQRLREEASVRKIPSQVEHSRLLGRLDQQI
                     SETRQALTKAQDALRDATYAVKRMLRWPADKPVQFELGDEELARILREAGADRLAALR
                     RQAAEAKIEQYRAAVPERYRSQASIGGVLDILRAVGVKALGPLYAVNFVMSVGVFDKQ
                     RGPLRDAAVFAYARAIVEAEELKREQAAENEKASFSLADLDRRIASAEEALFEMEERI
                     ADVQRAVDRDEAPMADLAELYKNRFEARRQLEALRRERTRMAKKLVEYEGPYVPVQPP
                     KVGTGEKIVNGAVSVVRAAMNPFDLLLSPFRKGKDKARLEALKQEIVRQDAPPVALRD
                     AGVDQLFELARARNPLFRQAQAYEREREMVLKAQKAGAWPKINTVLRYSSSVDPGGLR
                     PQSFGDLFTSGQLSYGLNATWRVYDPSRGSRVLIEQMEVEKADLGANDIASQVYLTIA
                     WQLETLERLQDSLPRLQGELNNVNDRLAQARQDQEFNIFSDPELARLEYEKARLEDEV
                     KVARARIQTLKIEIKKTAGLTDADSLEFSDTYSIRDLHHRLALQRFDRYVQETEGRVE
                     MNAEGKKVVDGVADTVDDARQYLALLQARQFLAVARSADKRSPALAGMEERLRGMEAA
                     VASRDLSLKDKTAQLNALLGEIRGLVKDIPALAKDSNSLLLGELSAKLAELEAALADP
                     SADPLTRLARLSEYSVYLTAVTASLTPELKWKTREGAALGLLLTAWDKALMGRFDPFV
                     SQRIAIRTAEIYQLQAMVANKMEPIELNMDFLTLFSTGDKKTTGTSVGSYFRQNALNF
                     VSIPPKLIETVFSPMHKAFHASRIGRIPVIGALLRDFIPGLKSSEARKAAIEEYMALA
                     LRELSSAQQKGVELQNARSAAIAHYRASVELLNLTRTRKVEFAMLSPYATLEEQKDAS
                     NQENVRLKYERDAQRAQEEVGEAELALLALGITPESLPPVAAPAGPALPLSLSAGRQY
                     ARDTFNVRMAELNKRIEAARLKKAEASSTLGRFQLMAGGQSAGWKFLPFFRFQLFSRE
                     SSTPYAVEAQRAEYRAAEQAVESEVLTQAGKLQDYYARLVESRLKAQLYDMLAGGSLA
                     EAEQLAGLSSREIDALTARVQRDIFRSEAARARADFSTEAELLSALSVTSRSLLLQAL
                     PSPQALTDALRALQDATMNRPVDVSAHPEVRRAQEMVRAEQLRRMDVALKAFIPDINL
                     DFQQNDFNNVLEVHLNASWRIIGSGTAARKGMADAEIAALQEKERQIRYDIESRIAQN
                     REEMSETLFELARVDGELKDLCDSARMQKLMSDYREGRIQIGELTAHLSSIGRLMGER
                     AYQLVRLAGLYESISQVFGAHGAPLPPLSELLKVSPEAASMDNLLKELGRKAPETPTE
                     QPVVLPAEWGASRSRTVPKGERPANVAPVPLWTVSKITTVGTRESPEESPSPIDVPPV
                     PGTDDDDQP"
BASE COUNT          970 a         1733 c         1772 g         1078 t
ORIGIN      
        1 ggctggtcgt cgtcgtcggt gcccggcacc ggcggcacat cgatcggcga cggcgactcc
       61 tcggggctct cccgggtgcc caccgtggtg atcttcgaga cggtccacag cggcacggga
      121 gcgacgttgg ccggcctctc ccccttcggg acggtgcggg accgcgacgc cccccattcg
      181 gcgggaagga ccaccggctg ttccgtcggg gtctccggcg cctttcggcc gagttctttc
      241 aacagattgt ccatggaagc ggcctccgga gaaaccttca acagttccga caggggcggc
      301 aagggcgcgc cgtgagctcc gaagacctgg gaaatactct cgtaaagacc cgccaggcgg
      361 acgagctgat aggcccgttc ccccatcagc cggccgatgg acgagaggtg cgccgtcaat
      421 tcgccgatct gtatccgtcc ttcgcgatag tcggacatca acttctgcat gcgcgccgaa
      481 tcacagaggt ccttcaattc gccgtccacg cgcgccagtt cgaagagcgt ttcggacatt
      541 tcttcgcggt tctgggcgat gcgggactcg atatcgtaac ggatctgtcg ttccttttcc
      601 tgaagggcgg cgatctccgc atccgccatc cctttccgtg cggccgtgcc ggacccgatg
      661 atccgccagg aagcgttcag atggacctcc aaaacattat tgaaatcgtt ctgttggaaa
      721 tccaggttga tgtcgggaat gaaggccttg agggcgacgt ccatccgccg taactgctcg
      781 gcgcggacca tttcctgggc gcggcgcact tccggatggg cggagacgtc caccggccgg
      841 ttcatcgtcg cgtcctggag cgcgcgcagg gcatccgtca gcgcctgcgg gctgggcagc
      901 gcctgcaaca gcagggaccg ggaagtgacg ctcaacgcgg aaaggagctc cgcttccgtc
      961 gaaaaatcag cccgggcgcg ggcggcctcg gaacggaaga tgtcgcgctg gacgcgggcg
     1021 gtgagggcgt cgatctcccg agaggacagg ccggcgagct gttccgcctc ggccaacgaa
     1081 ccgccggcca gcatgtcgta aagctgggcc ttcagacggc tctccaccag ccgggcgtaa
     1141 tagtcctgca gtttgccggc ctgggtcagg acctccgatt ccaccgcttg ttcggcggcg
     1201 cgatattccg ccctctgggc ctcgacggcg tagggggtgg aagattcgcg gctgaacaac
     1261 tggaagcgga agaaaggaag gaatttccag cccgcggact ggccgccggc catcaactgg
     1321 aaacgtccca gcgtgctgga agcttcggcc ttcttcagtc gggcggcctc gatgcgtttg
     1381 ttcagttcgg ccatccgcac gttgaaggtg tcccgggcat attgacggcc ggcagacagg
     1441 gaaagcggga gagccggtcc ggcgggggcg gcgaccggag gaagggattc gggggtgatt
     1501 cccaaagcca gcagcgccag ttccgcctcc ccaacctctt cctgggcccg ctgcgcgtcc
     1561 ctttcgtatt ttaaacggac gttctcctga ttcgaggcat ccttctgttc ttccagcgtg
     1621 gcgtagggcg acagcatggc gaactccact ttgcgggtcc gggtaagatt cagaagttcg
     1681 acggaggcgc ggtaatgggc gatggccgcg ctgcgggcgt tctgcaactc gacccctttc
     1741 tgctgggcgg aggagagttc ccgcaaagcc agggccatat attcctcgat ggccgccttg
     1801 cgggcctcgc tcgacttgag gcccgggatg aagtcccgca agagcgctcc gatcacgggg
     1861 atccggccaa tccggctggc gtggaacgct ttgtgcatgg gcgagaaaac ggtttcgatc
     1921 agtttcggcg gaatgctcac gaagttcaac gcgttctgcc ggaagtagga accgacggat
     1981 gtccccgtgg ttttcttgtc cccggtggaa aacaaggtga gaaaatccat gttcaactcg
     2041 atgggttcca tcttgttggc caccatggcc tgcagctgat agatctccgc cgtgcgaatg
     2101 gcgatgcgtt ggctgacgaa cggatcgaaa cgtcccatca gtgctttgtc ccaggccgtc
     2161 agaaggagtc ccagcgcggc gccttcgcgc gtcttccatt tcagttccgg cgtcaaggaa
     2221 gcggtcaccg ccgtcaaata gaccgaatac tccgaaagcc gggccagtcg cgtcaaagga
     2281 tcggcggacg gatcggcgag ggccgcctcg agttccgcga gcttggcgga gagttccccc
     2341 aacaacaagc tgttggagtc cttggccagg gcggggatgt ccttcaccaa tcctcggatt
     2401 tcgccgagga gggcgttcaa ttgggcggtc ttgtctttta agctgagatc ccgggaagcc
     2461 acggcggctt ccatgccgcg gaggcgttct tccatgccgg ccagcgccgg gctgcgtttg
     2521 tccgcggaac gggccaccgc caggaattgt cgggcctgca agagcgccag atactggcgg
     2581 gcatcgtcga cggtgtcggc cacgccgtcc accactttct ttccttccgc gttcatctcc
     2641 acacggcctt ccgtttcctg cacgtaccgg tcgaaccgct gcaacgccag ccggtgatgg
     2701 aggtcccgaa tcgaatacgt gtccgaaaac tccaaagaat ccgcgtcggt caagccggcg
     2761 gtctttttga tctcgatctt caacgtttgg atccgcgccc gggcgacctt gacctcgtcc
     2821 tccaggcgag ccttctcgta ttccagtcgg gccagctccg gatcgctgaa gatattgaac
     2881 tcctggtcct gacgcgcctg cgcgagtcgg tcgttgacgt tgttcaattc cccctgcaga
     2941 cgcggcaggg aatcctgaag ccgttccagc gtctccaact gccaggcgat cgtcagatag
     3001 acctggctgg cgatatcgtt ggccccgagg tcggcctttt ccacctccat ctgttcgatg
     3061 agaacgcggc tgccgcgcga cgggtcgtag acgcgccagg tggcgttgag cccataggaa
     3121 agctgaccgg acgtaaacag gtccccgaac gactgcggcc gcaacccacc gggatcgacg
     3181 gaagacgagt agcgcaagac ggtattgatc ttgggccagg cgcccgcttt ttgggctttc
     3241 agcaccatct cccgttcccg ttcgtacgcc tgggcctgac ggaaaagcgg gttgcgcgcc
     3301 cgcgccaatt caaacagctg gtccaccccc gcatcgcgca gggccaccgg cggagcgtct
     3361 tgtcggacga tctcctgttt caaggcctcc agccgcgcct tgtccttgcc cttccggaac
     3421 ggggaaagca gcaggtcaaa aggattcatg gccgcgcgaa cgacgctgac ggcgccgttg
     3481 acgatctttt cccccgtgcc caccttcggc ggctgcaccg gcacataagg gccttcgtat
     3541 tccaccagtt tcttggccat gcgcgtccgt tcccgccgca acgcctcgag ctgacggcgc
     3601 gcctcgaagc ggttcttata aagttcggcc aggtcggcca tcggggcctc atcccggtcc
     3661 acggcccgct ggacgtcggc gatgcgttct tccatctcaa aaagggcctc ttcggcggag
     3721 gcgatgcgcc ggtccagatc cgccagggag aaggacgcct tctcgttttc cgccgcctgt
     3781 tcgcgtttaa gttcctccgc ctccacaatg gcgcgggcat aggcgaacac cgcggcgtcg
     3841 cgcagggggc cgcgttgctt atcgaacacg ccgaccgaca tcacgaagtt gacggcgtac
     3901 agggggccga gcgccttcac gccgacggcg cgaaggatgt ccagcacgcc gccgatggac
     3961 gcctggctac gataccgttc gggcacggcg gcgcgatatt gctcgatctt ggcttccgcc
     4021 gcctgccgac ggagcgcggc caggcggtcc gcgccggcct cgcgtaaaat cctcgccaac
     4081 tcctcgtctc ccagctcgaa ttgaacgggt ttatccgccg gccaccgcag catgcgtttg
     4141 acggcatagg tcgcgtcccg gagagcgtcc tgcgccttgg tcaaggcctg gcgggtctcg
     4201 ctgatctgct ggtcgagacg gccgagcagg cgggaatgct cgacctggct ggggatcttg
     4261 cggaccgagg cctcctcgcg cagacgttgc aattcctgaa ggtgcgcggt ggccagcgcg
     4321 atgttctcct gggcggtccg gatgtcgtaa aaatgttcca caagcttcag ggactgctgc
     4381 gcgtgttcca cctcgtacaa aatctccgcc tgtttgttgc cccatcgcaa ggcgttcttc
     4441 tgggtcatgg tctcggagtc gaacaactgg tagcgcaccg tcatctcggc gcggaagcgc
     4501 ggttccacca gcatctcctc cagcgtggta tgccgcgcct gggcttcgcg cagcagataa
     4561 agcaggaaca tgaaatcgcc ttccgtcagt ttcacgtccc tgtcgaacgc cgtcgtctgc
     4621 acgtattccc ggaacagcgg ctgaatttgg gcgaccttct gcacaatctg gttgatggtg
     4681 aggccggtct ccttttccag catggcccgg ttcagcgaca ggaagaccac gatgctgttc
     4741 accgcctgga cgcgcggatc attgagggtc aggaggtcga accgggtgag gggggtgtcg
     4801 tccaggacac cgtcgccgtc gcggtccacg cgcaggcggt tcacgccgaa aaggtcgaat
     4861 agggtctggt cggtgatggc gccgtagttg ccgaagaacg tgctggccag gatggcgttg
     4921 gcgggcacct ggatcttatg tccgttgcgg tcggtgaaag tgaaatcgcc gccctcgttg
     4981 aggagccgct ggaactcctc caggttcagg acggtggcga aggcgtccat gaaatccttc
     5041 atcaacatct ccgtcacgcg ggtatcctcg gacaacgcga aggtccgcgc ctcgccgtcc
     5101 tgcacgagcc gtatcaccac gtcgtcgggc acgccgtcgc cgtccgaatc caccacgctg
     5161 gtggacatgc cggccatgaa cgccgccagc ccgatgatgc gctgggcttc ggagcggagg
     5221 aacggatcgg cgtcggggtc gccggaaatt tcgaaaagtt caagggcgtt cttgcccttg
     5281 aatatgtcgg cggcgcgcgg gtcgacgagg cgctgaaaac tgggcaggtt gagtatgaaa
     5341 tcctgcagga ggaggaactc gttgagatcc atgctctcgc gctgccggcc cgacaaatct
     5401 attttatact gatccccctc gattttcccc tgggtggaga aggacgacag aacgcccatg
     5461 gccgtggtag cctggcggcg gacctcctcg gaaagcgcgg ggtcgttggc ttgtgcgagg
     5521 agctgctgat agaaggtatt ggagagaagc ttg
//
LOCUS       DAOTFD010000175         5520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10577_length_5520_cov_3.967795, whole genome shotgun sequence.
ACCESSION   DAOTFD010000175 DAOTFD010000000
VERSION     DAOTFD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10577_length_5520_cov_3.967795"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="P5079_09095"
     CDS             <1..700
                     /locus_tag="P5079_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30176.1"
                     /translation="VLLARLRRAVRRDPGRFQKLTFSYRQRMDFPTRAGLGRTRNDVY
                     ESLNQNTRSKDVRGYVVGSALVCNHYWGISASHDEYVKTGPSDRFHHQTTVLGLGYSP
                     LGKNQGRIWNYSVDPAVSFEKFHGLEPVAGVRYESFGYSIGPNFYYGLDWIRLGRQTW
                     FTLDRFKVGTAISDSEIAKFAVSAGVNLTAYATRYLKLSLGVNDAYTPHDGKHQLNLK
                     TGLSVRLVWADWHF"
     gene            complement(741..2639)
                     /locus_tag="P5079_09100"
     CDS             complement(741..2639)
                     /locus_tag="P5079_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRY30177.1"
                     /translation="MGDGLKTDHFDVLLDLLDVEREAEKEENKRALDRLGVEVREALG
                     KTVTGLRIEKEDVGVGGIPLLVLSRPPAGETLAPFHAMDQGDLVRLAFDDGAPPLDGT
                     LYDVDEYRVSVALNDWPAGRPAGRVRVDLLGSDATYQRMRRALEHVRGAGGRLGALRD
                     IFLGWKAPQSGAAGESAFFNPRLNAWQKDAVRRALAAKHVAVVHGPPGTGKTTVLVEF
                     VRQAVRDGDRVLASAPSNVAVDNMLEKLLDAGMDAVRLGHPARTLDSLRHATLMVRAA
                     EHPDHRYIRELDQERERLMVQAARKAERGAGLTPRDEKELHKKIQSLWREARKWERGL
                     EKGILQNAQVVLATHGGLGKSMLREKFDVAVLDEASQATEPLSWIPISLADKVVFAGD
                     PLQLPPTIYSPEAARGGLKTTLMERLSESLPQAMRTLLRVQYRMNKTIMGFSSERFYE
                     GKLEADESVRDHLAVHLPGVLENELTRGPLVYVDTAGTGFSEVWNELLDSRENEGEAA
                     LALRLWNELEAAGIKRRQAAIISPYAAQVKILKGRAPRGLEVGTVDGFQGREKEVVLV
                     SLVRSNEKGEVGFLGDTRRMNVAMTRARRLLVVIGDSATISQHPFYAAFLDYAEQNGS
                     YRSAWEWR"
     gene            complement(2861..4804)
                     /locus_tag="P5079_09105"
     CDS             complement(2861..4804)
                     /locus_tag="P5079_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY30178.1"
                     /translation="MSTFNAGSGSFKKRFLRVLSLLGAASLPICLAAQTKTLRVGVYQ
                     NSPKVFVDAKGAPQGVFVDVVNEIARAEGWKIEYVPDTWHELLRRLERNEIDVLVDVS
                     FSRARAEKFVLNNIFVLESWLDAYTRKDDRIESVQDLGKKRIAVLEGSVQEEYLREDI
                     RKYFDIDFTLFPYPTYPASLKALKSGEVDVMVASRFFYFSDAMDEGIVQAHVVLRPEN
                     LYFAFPKGVDPHRVAAFDRRIASMKNDPNSAYYRSLHRWLDRQPRVMIPRYIKWALAG
                     ITGLLLGVGLFAYLLRQQVKMRTLALRGSEQRYRTTLNSIGDMICVVEKDLRVLLANA
                     VLVDFARRQGLSPAVTGRTLSEVFPFLSPKIAEENRKVFETGKPIVTEEAIPGKEGEL
                     IVEAMVMPITVDGRVAQAVTVVRDITARKLAQRSEEWTQKEILNALTRQQALIGQTLH
                     DSLGQLLTGAAHMVKSLDADLRAERPVHAAETARIVEVLNKGVAETRSLSRMLYPVEL
                     EKGDLVSALKEFAEQTSGVYKLKCALRVEGDYQQKSELVNTHLYRIVQEAVHNAVSHG
                     KATEIEIVLAGKGGVASLVITDNGTGFDVDSVEGKGLGLKTIKYRANEVGGRLSIERN
                     SPRGMRVECHFPHQGVEGNQGRA"
     gene            complement(5032..>5520)
                     /locus_tag="P5079_09110"
     CDS             complement(5032..>5520)
                     /locus_tag="P5079_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY30179.1"
                     /translation="ALVAATEKLEKGGDVPPETLRQVVEFLRTYADKFHHGKEEAHLF
                     ALLEKKGVPISGCPLGALLHEHKTGRALVGAFAEAVEAYAKDRPSGRARLAESMKPLA
                     ELYPSHIWKEDYLLSPMTGKVLNEADQKEILDKFIAVEKELGEDLHRKWEQFAENLGK
                     SL"
BASE COUNT         1086 a         1871 c         1444 g         1119 t
ORIGIN      
        1 cgtgctcctg gcccgcctcc gccgggccgt ccgccgcgac ccgggccggt tccagaaact
       61 gactttcagc tatcggcaac gcatggattt ccccacgcgc gccggcctgg gacggacgcg
      121 gaacgacgtg tatgaatccc tgaaccaaaa cacccgttcc aaggatgtcc gcggctatgt
      181 cgtgggttcc gccctcgttt gcaatcacta ttgggggatc agcgcctcac acgatgaata
      241 tgtgaagacc ggccccagcg accggttcca ccaccagacc accgtcctcg ggctcggcta
      301 cagccccctg gggaagaacc aagggagaat ttggaactac tctgtcgatc ctgcggtaag
      361 ttttgagaag ttccacggac tggaacccgt ggcgggcgtg cggtacgaaa gtttcggata
      421 ttccatcgga ccgaatttct attacggcct ggattggatc cgtctcggtc gtcaaacatg
      481 gttcacgctg gatcgtttca aggtcggcac ggcgatctcc gattcggaaa tcgcgaagtt
      541 cgcggtttcc gcgggcgtca acctcaccgc ctacgccacc cgctacctca agctctccct
      601 gggggtcaac gacgcgtata ccccgcatga cgggaaacac caattgaacc tgaaaaccgg
      661 tttaagcgtc cgcctggtct gggcggactg gcacttctaa gaacccgtcg cccgcttgtc
      721 tcccgcaacc cgcgtgtttc ctaccgccac tcccaggcgc tgcggtaaga accgttctgt
      781 tcggcatagt ccaggaacgc ggcgtagaac gggtgctgcg aaatggtggc cgaatcgccg
      841 atgacgacca agagccggcg ggcgcgcgtc atggccacgt tcatgcggcg cgtgtccccg
      901 aggaatccca cctcgccctt ttcgttggac cgcaccagcg acacaagcac gacctccttt
      961 tccctcccct gaaatccgtc cacggtgccg acttccagcc cgcggggcgc ccggcctttg
     1021 aggatcttga cctgggcggc gtagggggaa atgatcgcgg cctgcctccg tttgatcccg
     1081 gcggcttcca actcattcca cagccggaga gccagggcgg cctcgccctc attttcccgg
     1141 ctgtccaaca gctcgttcca aacctcggaa aaacccgtgc cggcggtgtc cacatacacc
     1201 agaggcccgc gcgtcaactc gttctccaaa acccccggca gatgcacggc caaatggtcg
     1261 cgcacgctct catccgcctc cagtttcccc tcataaaatc gttccgagga aaaacccatg
     1321 atggtcttgt tcatacggta ttgcacgcgc agaagcgtcc tcatcgcctg gggcaggctc
     1381 tccgagagcc gttccatcaa cgtcgtcttc agtccgcccc gggccgcctc cggcgaatag
     1441 atcgtcggcg gcaactggag cggatccccg gcgaaaacca ccttgtccgc cagcgaaatg
     1501 gggatccagg acaacggttc ggtggcctga gaggcctcgt ccaacacggc cacatcgaac
     1561 ttctcccgga gcatgctctt cccgaggccg ccgtgcgtgg ccaggaccac ctgggcgttc
     1621 tgcaaaatgc ctttctccaa gccgcgctcc cacttgcgcg cttcccgcca caacgactgg
     1681 atctttttgt gaagttcttt ttcgtcacgg ggcgtcagcc ccgccccgcg ttccgccttg
     1741 cgcgccgcct gcaccatgag ccgctcccgc tcctggtcca gttcgcggat gtatcgatgg
     1801 tccggatgct cggcggcgcg caccatcaac gtggcgtgcc ggagcgaatc cagagtgcgg
     1861 gcgggatgtc ccaggcggac cgcgtccatc ccggcgtcca aaagtttttc cagcatgttg
     1921 tccacggcca cgttggacgg cgccgacgcc aaaacgcgat cgccgtcccg gaccgcctgc
     1981 cgcacgaact ccaccagcac cgtggtcttg cccgtgccgg gcggcccgtg caccaccgcg
     2041 acgtgtttgg ccgccaaggc gcgacgcacc gcgtccttct gccaggcgtt caatcgcgga
     2101 ttgaaaaaag ccgactcgcc ggcggcgccg gactgcggcg ctttccaacc caagaaaatg
     2161 tcccgcagcg cgcccaaccg gccgccggcg ccccgcacgt gttccagcgc ccgacgcatc
     2221 cgctgatacg tggcgtcgga acccagcaaa tccacccgca cgcgacccgc gggccgtccc
     2281 gccggccaat cgttcagggc cacggagacg cggtattcgt ccacgtcgta aagggtgccg
     2341 tccagcggcg gggcgccgtc gtcgaaagcg agccgcacca aatcgccctg gtccatggcg
     2401 tggaacggcg ccaacgtctc ccccgccggc gggcgcgaca gaaccaggag agggatcccc
     2461 ccgacgccca cgtcctcttt ttcgatgcgg agcccggtga cggttttccc cagcgcctcg
     2521 cgcacttcca cgccaaggcg gtccagcgca cgcttgttct cttccttctc cgcttcccgc
     2581 tcaacgtcga gcaggtccag aagaacgtca aaatggtcgg tcttcaatcc gtcgcccata
     2641 ccgtctcttt ccaaaaaaat aacccgtgcg aaaaccggca cgggggtcaa aaaaccgcct
     2701 gtcgttaaat catatctcaa atggccataa aaatgacacc ccccctccgc cgcgcgccag
     2761 aaaagagtgc tttttaaaaa agaaatcgat tttctggttt tgagtcatta cgcgttatcc
     2821 cagccatgtc aacctcgaca ccactcccat gtttcaaaaa ctaagcccgc ccctgattcc
     2881 cctcgacccc ctgatgcggg aaatggcatt ccaccctcat accgcgcggg gagttcctct
     2941 caatcgagag ccgcccgccg acctcgttgg cccgatattt gatggtcttc aggcccagtc
     3001 ctttcccttc caccgaatcc acgtcgaaac ccgttccatt gtcggtaatc accaacgacg
     3061 ccacgccccc cttccccgcc agaacgatct cgatctccgt ggcctttccg tgcgacaccg
     3121 cgttatgcac cgcttcctga acgatccggt aaagatgtgt gttgacaagc tcgcttttct
     3181 gttgataatc cccctccacc ctcagggcgc atttcagctt gtatacgccg ctcgtctgct
     3241 cggcgaattc ctttaacgcg gagacaaggt cccctttttc cagttcgacg ggatagagca
     3301 tgcgggagag gctcctcgtc tccgcgaccc ccttgttgag gacttccacg atgcgggcgg
     3361 tctctgccgc atgaaccggc cgctccgccc tcaagtctgc atcgagggac ttgaccatgt
     3421 gcgccgcgcc ggtcaggagt tgccccaggc tgtcgtgcag cgtctgcccg ataagggcct
     3481 gctgcctcgt gagggcgttg agtatctctt tttgcgtcca ctcctcggat cgttgcgcaa
     3541 gcttgcgtgc ggttatgtcc cgcaccacgg taacggcctg tgccacccgc ccatccaccg
     3601 ttatcggcat caccattgcc tcgacgatga gttccccctc cttcccggga atggcctcct
     3661 ccgtgacgat gggtttcccc gtttcaaaaa ccttccgatt ctcctccgcg atcttcgggg
     3721 acagaaacgg aaaaacctcg gacaacgtgc ggccggtcac ggcgggggac aggccttgcc
     3781 ggcgggcgaa atccaccaac accgcgttcg ccagcaagac ccgcaggtcc ttttccacca
     3841 cgcagatcat gtcgcctatg gaattgagcg tggtgcggta tcgctgttcc gagccgcgca
     3901 gagccaacgt cctcatcttc acctgctgcc gtaaaaggta cgcaaacaat ccgacgccca
     3961 acaacaatcc cgttatcccc gccagggccc actttatata gcgcgggatc atcacccgcg
     4021 gttgccggtc cagccaacgg tgcaacgacc ggtaatacgc cgagttcggg tcgtttttca
     4081 tcgaagcgat gcggcgatcg aaagcggcga cccgatgggg atcgacccct ttcgggaacg
     4141 cgaaataaag gttttccggc cgcaacacga cgtgcgcctg cacgatccct tcgtccatgg
     4201 cgtccgaaaa atagaaaaac cgggacgcga ccatcacatc gacctcgccg gactttaggg
     4261 cttttaaact ggcgggatag gtcggatagg gaaacagcgt aaaatcgatg tcgaagtatt
     4321 tccttatgtc ctcgcgcaga tattcctcct gcaccgatcc ttccagaacg gcgatcctct
     4381 tcttgcccaa gtcctgcacc gattctatgc gatcgtcttt ccgcgtatag gcatcgagcc
     4441 aggattccag aacgaagatg ttgttcagga cgaatttttc cgcccgggcc cgcgaaaagg
     4501 atacatccac cagcacgtcg atttcattcc gttccagccg gcggaggagt tcatgccagg
     4561 tgtcggggac atactctatc ttccaacctt ccgcgcgagc gatttcattg acaacgtcga
     4621 cgaatacccc ttggggagcc cccttcgcgt ccacgaaaac cttgggactg ttctgataga
     4681 cacccacccg caacgtcttt gtctgcgccg caagacatat cggcaaggat gccgccccca
     4741 ggagcgacag gacccgaagg aaccgttttt tgaacgagcc ggaaccggcg ttaaaagtcg
     4801 acatagaacc gacgtccttt cttttcatac ataaataaag gccggtccct gtcccgcggt
     4861 cgggaataga accagcctct ttattacggt ttacgcctgc actatgaaaa aaatgtcaca
     4921 acacagacag aaatccggat cccttattgc gccggattag aacctgttta cagcaagccg
     4981 cttcccccta tcaccaaaat actcccattt cccctggttc accacaacct actataatga
     5041 tttcccgaga ttttctgcaa attgttccca ttttcgatgg agatcttcgc ccagttcctt
     5101 ttccaccgca ataaatttat ccaggatttc tttttggtcc gcttcgttta gaaccttccc
     5161 cgtcatgggg gaaaggagat aatcctcttt ccaaatatgg ctggggtaga gttccgccag
     5221 gggcttcatg ctttcggcaa ggcgggcccg gccggacggc cggtctttgg cataggcctc
     5281 aacggcctcg gcgaacgcgc cgacgagcgc ccgcccggtt ttgtgctcat ggagaagggc
     5341 ccccagcgga catcccgata tcggaacccc cttcttttcc aataacgcga aaaggtgggc
     5401 ctcttctttc ccgtgatgga atttatccgc atacgtccgc aggaactcca ccacctgccg
     5461 cagggtttcc ggtggcacgt ccccgccctt ttccagcttc tccgtcgcgg cgaccagagc
//
LOCUS       DAOTFD010000176         5516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10592_length_5516_cov_3.934994, whole genome shotgun sequence.
ACCESSION   DAOTFD010000176 DAOTFD010000000
VERSION     DAOTFD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10592_length_5516_cov_3.934994"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5079_09115"
     CDS             complement(<1..591)
                     /locus_tag="P5079_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30180.1"
                     /translation="MMARWLESKRLALSLVFSFLTSLSWAGVWVVADFNSGNTPLNAG
                     PSSNISDIGSASHAFRTDSASEPAVGRRGYSLKITHSASGGSFLVLDFASPVNVSTYS
                     TLSFWVRGAVGSEKFQVSLKRNTAPNEPNFRPDIRNFLSVGVSTGWQKVTIPLRSLMA
                     DWVVNTSTALSDVTGVVFTVDAAVPTESFYIDDVLFH"
     gene            complement(641..1861)
                     /locus_tag="P5079_09120"
     CDS             complement(641..1861)
                     /locus_tag="P5079_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30181.1"
                     /translation="MRRLLFAAALLLPTTPLSSDELLNRACYDALKTFRVPNAHEFPC
                     DNTGRGGRTYTKATNIGLDIVSTLAAWETGLVSYKEAHAHIVKLLEGLESLKTYHGIF
                     PEYIKLENGRAVPDAQNGAVRYSSIDSAWLHFGLSLAAATLRKDAPTTAARADLLLKE
                     ADYSVFLHDGNRFLRHGLTVAAGNAPRVTDFWPYNYDNKNSEARVLVLFLTAAGKLPP
                     TVWDNMHYRAATVRGHTVAEGWKMSAFVEMVGNSYFDELRLAPKSFGASHLAYLKACL
                     QVAEEEGLTLFGWAPCFGPDDNYREYGLEKFSVATPYAAALLTTLNAFETTKNFKKML
                     EFLPVTDTPKPFPDALDSKTGEVVNTRALALDQNLLFHALVKDTTRKIVSRAEWHKEA
                     CRLLSSTDERHPLR"
     gene            complement(1861..2685)
                     /locus_tag="P5079_09125"
     CDS             complement(1861..2685)
                     /locus_tag="P5079_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY30182.1"
                     /translation="MVGVGTHPDPDLKEHYFSETFRGLRQALGRRSRCSLVVNPPPGR
                     AVDALILLAPTEDDPLLRRARRDNIPTVLVNGCSHGFPCVDLDNITAAFAAVSHLLRL
                     GHRRVGMINGKMETANGRDRYEGYRRALAAHGISLDETLVTEGRFSRAGGRTAMERLL
                     SLPHRPTAVFAANDHMALGALEAARHAGRRVPADVAVVGFDDIAAAASSSPSLTTVRQ
                     PLFQMAREAANLLAPNDPPARREGPGNPSSRRILLQGTLVVRKSCGAFPEKFFSAD"
     gene            complement(2780..4219)
                     /locus_tag="P5079_09130"
     CDS             complement(2780..4219)
                     /locus_tag="P5079_09130"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY30183.1"
                     /translation="MRQKIFLLRRSLLIPCLAAAAMVAGGAGPARASEGQYLPEKDQQ
                     ARLIRFPPAQHKKIDSALPEKKFLLQIASDTWGYFRDVTDRETGLPLDNVVIGSTYTK
                     VGSFTSTTNIGLYLMCVVSAVDFGFISSTEAVHRLRLVMESLNKLETWENQPYNFYDT
                     IDLTNSGKYVSSVDNGWLAAGLIVARQAYPDEFGAEMDMWLDRLDFSKLYDPRNAQLF
                     GGYDAGHGKHSENHYGLLCTEPRLTSYIAIAKGDVPREHWFRLFRTLPKDWDWQNQTP
                     RGVERKYSGWPVFNGHYRYGRIQFVPSWGGSMFEFLMPTLVLDEQRLAPEGLGLNNQR
                     ILSVQIHYALMRKRYPVWGMSPCAIPEETLGYREYGVTALGSKGYEDDGVVTPHATFL
                     ALAVDPAQAVKNLRRLAKIPGIYGEFGFYDSVNVRTGQVSPRLLCLDQGMSFIALANY
                     LNDGAVRKRFHSYPPIQEQEDLLTVEKFF"
     gene            complement(4533..>5516)
                     /locus_tag="P5079_09135"
     CDS             complement(4533..>5516)
                     /locus_tag="P5079_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRY30184.1"
                     /translation="ARTGRRAYTIPVDPEDPLVKKYIAIFTGPRKKEMEEALSRLTIY
                     RPMILKHLKEMNLPRELLYLPVIESGVKNTATSRAGAVGLWQFMSSTGRGYGLKINYW
                     LDERRDPEKSTAAGLRCLKDLHEWFDDWHLALAGYNRGVYGVERDMKFSRSPDFNKLA
                     ERKALPGETEHYVPKYMACVLIADDPAAYGLTVRELAPPAVDEVTLEKPLDLRVAAEA
                     AGVPEAVLQELNPTVRMWCTPKNEPNFPFRIPAGTRERFVMTLSRVKDWTPFSGEVKY
                     KVKKGDMLSRIAQRYKTSAREVQRLNRIKNPQFLRPGQVLIIRPGKGFRGN"
BASE COUNT          961 a         1613 c         1855 g         1087 t
ORIGIN      
        1 atggaacaac acatcgtcga tgtagaaact ttccgtagga acggcggcgt ccaccgtgaa
       61 tactacgccg gtgacgtcgc tcagggcggt gctggtgttg accacccagt ccgccatcag
      121 ggagcgcaag gggatcgtca ccttctgcca accggtggaa acgccgacgg atagaaagtt
      181 gcgtatgtcc ggccggaagt tgggttcgtt gggggcggtg ttgcgtttca gagagacttg
      241 gaatttttcc gatcccacgg cgcctcgcac ccagaaagag agggtactgt aggtggagac
      301 gtttacgggg gacgcgaaat ccagcaccag gaaactgcca ccgcttgcgc tgtgggtgat
      361 ctttaaagaa taaccccgtc ggcccacggc cggttcagaa gcgctgtcgg tccggaaggc
      421 atgggaagcg ctgccgatgt cggaaatgtt gctggagggg ccggcgttga ggggcgtgtt
      481 tccgctgttg aaatccgcca ccacccacac cccggcccag gacagagagg tgaggaaaga
      541 gaagacaagg gaaagcgcga gacgttttga ttccagccag cgagccatca tgaaggtcgg
      601 tgtggtcttg tcccctgttt tttgaggttc ccctcccccg tcatcgcagc ggatgccgtt
      661 cgtccgtgga actcagcaga cggcaggctt ccttgtgcca ctccgcccgg gacacgatct
      721 tgcgcgtcgt gtccttcacc agcgcgtgga acagcaggtt ttgatccaag gccaaggccc
      781 gggtgttgac cacttctccc gtcttggaat ccagcgcgtc tgggaacggt ttgggggtgt
      841 ctgtaacagg gaggaattct agcattttct tgaagttttt agtggtctca aacgcattca
      901 gagtggtcaa aagggcggcg gcgtaagggg tggcgaccga gaacttttcc aagccgtatt
      961 cgcggtaatt gtcgtccggc ccgaaacacg gcgcccagcc gaacagcgtc agtccttcct
     1021 cctccgccac ctgcaggcag gccttgagat aggccaggtg gctggccccg aacgatttcg
     1081 gcgccaaacg gagttcgtcg aaatagctgt tccccaccat ctccacgaag gcgctcattt
     1141 tccagccttc ggccacggtg tggccgcgga ccgtggcggc gcggtagtgc atgttgtccc
     1201 acaccgtggg cggcaatttc cccgccgccg tcaaaaacag caccagcacc cgcgcctcgc
     1261 tgttcttgtt gtcgtagttg taaggccaga aatccgtcac ccggggggcg ttcccggcgg
     1321 cgaccgtcag gccgtgccgg aggaaccggt ttccgtcgtg gaggaacacg gaatagtcgg
     1381 cttctttgag gagcaggtcc gcccgggcgg cggtggtggg ggcgtccttg cgcagggtgg
     1441 cggccgccag gctcagcccg aaatgcagcc aggcggagtc gatggaggaa tagcgcaccg
     1501 cgccgttttg cgcgtccggg accgcgcggc cgttttccag tttgatgtat tcgggaaaga
     1561 tgccgtgata ggtcttcagg gattccagcc cttccaacag tttcacgatg tgggcgtggg
     1621 cttctttata ggataccagg cctgtttccc aggccgccag ggtggagacg atgtccagcc
     1681 cgatgttggt ggctttggtg taggtgcggc cgccccggcc ggtgttgtcg caggggaact
     1741 cgtgcgcgtt ggggacgcgg aaggtcttca gggcgtcgta gcaggcgcgg ttcaggagtt
     1801 cgtcggaaga caggggtgtc gtcggaagga gaagggcggc ggcgaacagg agacggcgca
     1861 tcagtcggcg gaaaaaaact tttcggggaa cgcgccgcag gattttcgga cgaccagcgt
     1921 cccctggagg aggatgcgcc ggctcgaagg gtttcccggt ccctcccgtc tcgccggcgg
     1981 gtcgttcggg gccagaaggt tggccgcctc gcgggccatt tggaacagcg gctgccgcac
     2041 ggtggtgagg gacggggagg aggaggcggc ggccgcgatg tcgtcgaacc ccaccacggc
     2101 cacgtccgcg ggcacgcgcc ggccggcgtg ccgcgccgct tccagggcgc ccagggccat
     2161 gtggtcgttg gccgcgaaca ccgccgtggg gcggtgcggc aacgacagca ggcgttccat
     2221 ggccgtccgt ccgccggcgc gggaaaaacg gccttccgtc accagcgttt catccagcga
     2281 tatcccgtgc gcggccaggg cgcgccgata tccctcgtac cggtcccggc cgttggcggt
     2341 ctccattttg ccgttgatca ttcccacccg gcggtggccc aggcggagca ggtgcgacac
     2401 cgccgcgaac gccgcggtga tgttgtccaa atccacgcag gggaacccgt gcgaacagcc
     2461 gttcaccagg accgttggaa tgttgtcccg ccgggcacgc cgcaaaagcg ggtcgtcctc
     2521 ggtcggggcc aacaggatga gcgcatcgac ggcccgtccg ggcggcggat tgacgaccag
     2581 cgaacaacgg ctccggcggc ccaaggcctg gcgcaggccg cggaaagttt cggagaagta
     2641 gtgttctttc aggtcggggt cgggatgggt ccccacgccc accacgcggc gtcgccgtgc
     2701 ggcgggactg tttttgcgcg cgcgggaacg ttttttcgcg gggagagaca tgcgggcggg
     2761 ccgagaaggg aagaaggcgt tagaagaact tttccaccgt caacaggtct tcttgttcct
     2821 gtatgggcgg gtagctgtgg aaccgtttgc gcacggcgcc gtcgttcaga taattggcca
     2881 gcgcgatgaa actcatgccc tggtccaggc agagcaggcg cggcgagacc tgtccggttc
     2941 gcacgttcac gctgtcgtag aaaccgaatt cgccgtagat cccggggatc ttggccagcc
     3001 gccggaggtt cttcacggcc tgggccgggt ccaccgccag ggccaaaaag gtggcgtgcg
     3061 gggtcaccac gccgtcgtcc tcgtagccct tggaacccag cgccgtcacg ccgtattccc
     3121 ggtaaccgag cgtctcctcc gggatcgcgc agggagacat tccccacacg ggatatcgtt
     3181 tccgcataag ggcgtaatgg atctgcacgc tcaatatccg ctggttgttc agacccagtc
     3241 cttccggagc gaggcgttgt tcgtcgagga ccagggtggg catcaggaat tcgaacatgc
     3301 tgccgcccca ggaaggcacg aattggattc ggccgtagcg gtagtgtccg ttgaacaccg
     3361 gccatccgga atatttgcgt tccacgccgc gcggcgtctg gttctgccag tcccagtcct
     3421 tgggaagggt gcggaaaagg cggaaccagt gttcccgggg aacgtccccc ttggcgatgg
     3481 cgatgtagga ggtgagccgt ggttcggtgc acaggaggcc gtagtggttc tccgaatgtt
     3541 tgccgtggcc ggcgtcgtag ccgccgaaaa gctgggcgtt gcgcgggtcg tagagtttgg
     3601 agaaatccag tcggtcgagc cacatgtcca tctccgcgcc gaattcgtcc ggataggcct
     3661 ggcgcgccac gatgagaccg gccgccagcc agccgttgtc caccgacgac acgtatttgc
     3721 cgctgttggt caggtcgatg gtgtcgtaaa agttgtaggg ctggttctcc caggtttcca
     3781 gcttgttcaa gctctccatc accagccgga ggcggtgcac cgcctcggtg gacgagatga
     3841 aaccgaaatc caccgccgac accacgcaca tgaggtagag gccgatgttg gtggtggagg
     3901 tgaagctgcc cactttggtg taggtggacc cgatcaccac gttgtcgagc ggcaggccgg
     3961 tttcgcggtc ggtcacgtcg cggaaataac cccaggtgtc gctggcgatc tgcagaagga
     4021 attttttttc gggcagggcg gagtcgatct ttttgtgctg tgccggcggg aaacggatca
     4081 gccgcgcctg ctgatctttt tcggggagat actggccttc ggaggccctc gcgggaccgg
     4141 cccctccggc caccatcgcg gccgcggcga ggcaaggaat gaggagggac cgacggagaa
     4201 gaaatatttt ttgtcgcata agagcgctca ttttaggcaa tccggacggg aagttcaagg
     4261 ttgaaggtcc gccggttttt taccgtcgag gtctcatgaa agacggggct tccggccccg
     4321 tcccccggcc aggatttctt ggcccgtctc aagaaatcct ggacaaagaa agacgccccg
     4381 tcggcttccc ctctccgttt tttcggcggt cgcgaactca ccgtcctcga ccgcccgtcg
     4441 ggcggctcga acatcgcgac ctgtccgctc cgaaaaaaca gctcggtcat gacggccgaa
     4501 agggggtgtt agagagagcg gaaatcacag gatcagttcc cgcggaagcc tttgcccgga
     4561 cggatgatca gcacctgacc ggggcggagg aactgcgggt ttttgatgcg gttgagccgc
     4621 tggacctccc gcgccgaggt cttgtaacgt tgggcgatgc ggctgagcat gtcgcctttc
     4681 ttcaccttgt atttcacttc gccggaaaaa ggcgtccagt ctttcacgcg gctgagggtc
     4741 atgacgaagc gttcccgcgt gccggcgggg atgcggaacg gaaagttggg ttcgttcttc
     4801 ggcgtgcacc acatccgcac cgtggggttc agctcctgca gcaccgcttc ggggacaccg
     4861 gccgcctcgg cggccacgcg caggtccaac ggtttttcca gggtcacttc gtccacggcg
     4921 ggcggagcga gttctctcac ggtcagcccg taagccgccg ggtcgtcggc gatgagcacg
     4981 caggccatgt atttgggcac gtagtgttcc gtttctcccg gcagggcctt gcgttcggcc
     5041 agcttgttga aatcggggga gcggctgaac ttcatgtcgc gttccacgcc gtacacgccg
     5101 cggttgtagc ccgccagggc caggtgccag tcgtcgaacc attcatgcag gtctttcagg
     5161 cagcgcagtc cggccgcggt ggatttttcc gggtcgcgcc gttcgtccag ccagtaattg
     5221 atcttcagcc cgtaaccgcg gccggtggaa ctcatgaatt gccacagtcc caccgccccg
     5281 gcgcgggagg tggcggtgtt cttcaccccg ctttcgatca cgggcaagta gaggagttcc
     5341 cgcgggaggt tcatctcttt caggtgtttg aggatcatgg ggcgatagat ggtcaagcgg
     5401 ctgagggcct cttccatttc ttttttgcgc ggtccggtga agatggcgat gtattttttc
     5461 accaacggat cttcggggtc caccgggatg gtgtaggccc gtcgtcccgt ccgggc
//
LOCUS       DAOTFD010000177         5508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10618_length_5508_cov_3.226481, whole genome shotgun sequence.
ACCESSION   DAOTFD010000177 DAOTFD010000000
VERSION     DAOTFD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_5508_cov_3.226481"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..119
                     /locus_tag="P5079_09140"
                     /pseudo
     CDS             1..119
                     /locus_tag="P5079_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
     gene            complement(413..2083)
                     /locus_tag="P5079_09145"
     CDS             complement(413..2083)
                     /locus_tag="P5079_09145"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRY30185.1"
                     /translation="MNEKRKGQKGLNLKAATRLALLFAVLTFSVSARASFEPLPVGAR
                     AAGMGEAYTVVADDTLSLYYNPAGIIHVRRPELGTSYSRLFFGLDDKSEISRSFLGYA
                     QPLPHNWGAAGISYLHLGLSGLYSEDTIGLSFAREVQDKWNVGGSLKLLRKSFGSDQY
                     TDNAINVETGQALNAKDPLLAKGNSKSAVGLDLGAQYRLSKNYAFGLALRNINQPNTA
                     LNGSDKAPAVYALGISRWNRVSSLSLELANWKFANDQDYRLNFGGERWFRSGFGARAG
                     LAFGSRQFANLSLGGSYRMDGFQFDYALHYPLQGVEKTAGTHMVSLSYRFGKPEPDPV
                     EAQLNSEREARLRAEAELARLRQQLMELTTEKPVAAPATDVVDKAAQEALKQAEEEIQ
                     RLKSQPQPVSAAPVETPAPAPTAVAPTAPVAPKPVAPPPAKPRVSPDLLAQYSDALKF
                     YSQQTKSGAPLEERIATLQRTIDKYEGKGLDISSVYTELKKLKGEDTKTSDDYKLAVS
                     YYNRIVQQGGTSAEERILLLERIVKKYKPMGVNTSDIEKELETLKKKR"
     gene            complement(2139..>2474)
                     /locus_tag="P5079_09150"
     CDS             complement(2139..>2474)
                     /locus_tag="P5079_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRY30186.1"
                     /translation="KLKFFGLAMTLLLLQGDIFALKIYSRIFTPNGDGWNDQVVFEME
                     NPGLLPLKGEIFDLTGAKVDDMIPGPNSETTLKWNGKKSNVPVPSGIYIYQIELGGKI
                     TTGTVVVAQ"
     assembly_gap    2475..2484
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2624..5164)
                     /locus_tag="P5079_09155"
     CDS             complement(2624..5164)
                     /locus_tag="P5079_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY30187.1"
                     /translation="MRLWLRLATAWLVVTQATFAPFEPLIRSRFFGGMASVEAATTDS
                     MTLSVSVVQNPPAAITNLTALEGDLLFDPNREGALTLNWTEPESGAGSGIPATSYHVR
                     YQTTPNIEDDADFAAATVYTQTWVPSSPGNNRAEVITGLEPGATYYWAIKAQNMIALQ
                     GTWSRGSAGDGINDNNFAIAYDTAPPAPANLTATPGTGQNQLAWNPVNVADLDFYRIS
                     VDSVTPYDFADAYTLTVDSVSITYTHSGLTNGTTYYYRVTAVDKGAPTYAGNALESVP
                     SNTVYAVPGLTLPNAPELMSDLSNLTTTQIRWLLTDRASNEDALYVYSPPANRSPNLG
                     PLPGTGLMNAYTESGLTPNTLYQRYGEAANVVGSSFSARVDVYTLANPPVNTRVTAVS
                     TSSVFLAWDSNNNPPNTLYEVQYSKNATFTNPQQAGSARATSFTITDLQSNTNYWVRV
                     RAQNESGYTTVFDAIVSTRTLKMMDTVPPKAPAGVWAEWVNSSGGRKVKLHWRAVSEN
                     MDNTPFNDPADEPYKVYRTASVSTTMATLQGAPQVPSITTTAAAEIEMDISAGDRYYY
                     RVRAVDITGNESPDSLMIEAVGDGSKLNVVALADDDQSRLTIPVGIARNLLLKEINGL
                     GDDVLVRAVEMSSEEKGKVVKSVQFEAFRADTGESMKDFLIRPAEGTIMVGYEVQNGQ
                     VLQGAPTVRGVPGAPLTDASLASQQLSLFWHNGVEWVKVGGVVDTSNQTVSIRTSRLG
                     RYQIRQAVRVGPATLTRVYPQIFTPNDDGWNDKVIFEFENTALLPLKGEIFDLSGAKV
                     GDMVAGPNPDTSLMWDGKRGGQPVPGGVYVYQIEVGGETVTGTVVVAK"
     gene            complement(5239..>5508)
                     /locus_tag="P5079_09160"
     CDS             complement(5239..>5508)
                     /locus_tag="P5079_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30188.1"
                     /translation="YYLSSVSPRDLSMPVGKDVEAPPDPSYIILPKKELRVDANTIEP
                     FHVFLEMPKQEEYRGKRYVFLLQVELPGETAILNQIARIYVNTEK"
BASE COUNT          970 a         1601 c         1752 g         1175 t
ORIGIN      
        1 acgagcccta ttggtaccgg aactttcact acgagcacga gcgcgagcgc gggctggatc
       61 acgtggaaga ccttgcgtcg cccggcgtgt tgcgcttcga cctctcggcg ggtgaggccg
      121 cctggatctg gttcaagcgg gagaggacga gcgtgctgag ttcctcgaag cgttccaatt
      181 ccgtggaatc gccgacgagg aagtaggcgt cgagagaacc tttttggcgg gcgaggagga
      241 cctgcaggct ccgcacctgg gaaaccttct ccatgtcgtc gagagattcc ctgaaaatat
      301 tgacgacccg ggtcccccct ccgacggcca acatccccag ggccaatata atccccgcca
      361 tcacggtgaa aaacaagccg agttgtgtgc gaagccgcat tgaaccggca gtctacctct
      421 ttttcttcag agtttccagt tccttctcga tgtcggaggt gttcacgccc atcggtttgt
      481 atttcttgac gatccgttcc aggaggagga tccgttcctc ggcgctggtc cccccctgtt
      541 gaacgatgcg gttgtaatag ctcaccgcca gtttgtagtc gtcggaggtc ttggtgtctt
      601 cgcccttgag ttttttcagt tccgtgtaga cggaggagat atccaggcct ttcccctcgt
      661 atttatcgat ggtgcgctgg agcgtggcga tgcgttcctc cagcggcgcc ccggacttgg
      721 tctgttggga atagaacttg agggcgtcgc tgtattgggc cagaaggtcc ggcgagacac
      781 ggggtttcgc cggaggcggc gccaccggtt tcggcgcgac cggcgccgtc ggcgccacgg
      841 cggtcggggc gggggcggga gtttccaccg gggccgcgga gaccggctgg ggctggctct
      901 tcagccgctg gatctcctcc tccgcttgct tcagggcctc ctgggcggcc ttgtccacca
      961 cgtcggtggc cggcgcggcg accggttttt ccgtggtcaa ctccatcaac tgttggcgaa
     1021 gccgcgccag ttccgcctcc gcccgcaggc gggcctcgcg ttcggagttg agctgcgcct
     1081 ccaccgggtc gggttcgggt ttcccgaagc ggtaactcag cgacaccatg tgggtgcccg
     1141 cggtcttttc cacgccctgc agcggatagt ggagggcgta gtcgaactga aacccgtcca
     1201 tccgatagga gccgccgagg gagaggttcg cgaactgccg gctgccgaag gcgaggccgg
     1261 cccgggcgcc gaaaccgctg cggaaccagc gttcgccgcc gaaattgaga cgatagtcct
     1321 ggtcgttggc gaacttccag ttggcgagtt ccaaggagag ggaggagacc cggttccagc
     1381 ggctgatccc cagcgcataa accgccgggg ccttgtcgct gccgttgagg gcggtgttgg
     1441 gctggttgat gttccggagg gccagcccga aggcgtagtt tttgctcaag cggtattggg
     1501 cgccgaggtc gagccccacg gcgctcttgg agttgccttt cgccagaagc gggtccttgg
     1561 cgttcagcgc ctggccggtc tccacattga tggcgttgtc ggtgtattgg tcggaaccga
     1621 aacttttgcg caacaatttc aggctgccgc ccacgttcca tttgtcctga acttcccggg
     1681 cgaaggagag gccgatggtg tcttcggaat agaggccgga caggcccagg tggaggtagc
     1741 tgatcccggc ggcgccccag ttgtgcggga ggggctgggc atatcccaaa aacgaccggc
     1801 tgatctcgga tttatcgtcc aaaccgaaga acagccggct gtaagaagtt cccaattccg
     1861 ggcggcgcac atggatgatg cccgccggat tgtaatagag agaaagggtg tcgtccgcca
     1921 ccaccgtgta ggcctccccc atgccggcgg cgcgggcgcc cacggggagc ggctcaaacg
     1981 aggcgcgggc ggaaacggaa aacgtcagga cggcaaaaag aagggccagc cgcgtggcgg
     2041 ctttgagatt cagccctttt tgtcctttcc gtttttcgtt cactttttcc tctcgaatca
     2101 ggagtcgaca gtcgacttcc tgtttttcat caaaaaaatt attgcgccac cacgaccgtc
     2161 ccggtggtta ttttcccgcc gagctcaatc tggtagatat aaatcccgga cggcaccggc
     2221 acattgctct ttttcccatt ccacttcaac gtcgtttcag aattgggccc cggaatcatg
     2281 tcgtccactt tggcgccggt caaatcaaag atctcccctt tcaaagggag aagtccggga
     2341 ttttccattt caaacacgac ttggtcgttc cagccgtccc cattgggggt aaaaatgcgc
     2401 gaataaatct tgagcgcgaa aatatctcct tgtaatagta atagtgtcat ggccaaccca
     2461 aaaaatttta atttnnnnnn nnnncaggat ggatttagcc ataaaaattt taaaacgcgc
     2521 tgcaagcttc atgttttcct ccaaaaaaaa ttcacttcca ctctaagctt aaccctcgaa
     2581 acctaaaatt tccaggggtg aatgcaaata atttacaact ctgttacttc gccaccacca
     2641 ccgtgcccgt cacggtttcc ccacccacct cgatctggta gacgtagacc ccgccgggca
     2701 ccggttggcc gcctcgtttg ccgtcccaca tcagggaggt gtcggggttg gggcccgcca
     2761 ccatatcgcc cactttggcg ccggagaggt cgaaaatttc gccttttaag ggcagaaggg
     2821 cggtgttttc gaactcaaag atgactttgt cgttccagcc gtcgtcgttg ggggtgaaga
     2881 tttgcgggta gacgcgggtc agggtggccg gaccgacgcg gacggcctgg cggatctggt
     2941 agcggcccaa ccggctggtg cggatggaaa ccgtctggtt gctggtgtcc accacgccgc
     3001 ccaccttcac ccactccacg ccgttgtgcc agaagaggga cagttgttgg gaggccaggg
     3061 aggcatcggt gagcggcgcc ccgggcacgc cacggacggt cggcgctccc tgcagcacct
     3121 ggccgttttg gacctcatac cccaccatga tggtgccttc cgccggccgg atcaggaagt
     3181 ccttcatgga ctcgcccgta tcggcgcgga aagcctcaaa ctggacgctc ttcaccactt
     3241 tccccttctc ctcggacgac atctccaccg cccgcaccag gacgtcgtcg ccgaggccgt
     3301 tgatctcctt cagcaggagg ttgcgggcga tgccgacggg gatggtgagg cggctctggt
     3361 cgtcgtcggc cagggcgacc acgttgagtt tgctgccgtc gcccaccgcc tcaatcatga
     3421 gggagtcggg ggactcgttc ccggtgatgt ccaccgcccg aacacggtag taatagcgat
     3481 cgccggccga aatgtccatc tctatttcgg cggcggcggt ggtcgtgatg ctcggcacct
     3541 gcggggcccc ctgcagggtc gccatcgtgg tggacacgga cgccgtccgg tagaccttgt
     3601 acggctcgtc cgccgggtcg ttgaacgggg tgttgtccat gttctccgac accgcccgcc
     3661 agtggagctt gactttccgt ccgccggagg agttgaccca ttcggcccac acgcccgccg
     3721 gggccttggg cggcacggtg tccatcatct tgagggtccg ggtggaaacg atcgcgtcga
     3781 acacggtggt atagccggat tcgttctgcg ccctcacgcg gacccaataa ttggtgttgg
     3841 attgcaggtc ggtgatggtg aagctggtgg cccgcgccga accggcctgt tgcgggttgg
     3901 tgaaggtggc gttcttggag tactgcacct cgtacagggt gttgggcggg ttattgttgg
     3961 agtcccaggc caggaacacg ctggaggtgg acaccgccgt cacgcgggtg ttcacgggcg
     4021 ggttggccaa ggtataaacg tccacgcggg cgctgaagga actgcccacc acgttggcgg
     4081 cttcgccgta gcgctgataa agggtgttcg gggtgagacc ggactccgtg taggcgttca
     4141 tcagcccggt tccgggcagc ggcccgaggt tgggcgaacg gttcgccgga ggcgaataaa
     4201 cgtacaaagc gtcttcattg gaagcgcggt cggtcaacaa ccagcggatc tgtgtcgtcg
     4261 tcaggttgga caggtcggac atcagttccg gcgcgttggg cagggtcagt ccggggacgg
     4321 cgtagaccgt gttggacggc acgctttcca gggcgttgcc cgcatacgtc ggcgcgcctt
     4381 tgtccacggc ggtcacccgg taatagtagg tggtgccgtt ggtcaggccg gagtgggtgt
     4441 aagtgatgct gacggaatcc accgtgaggg tgtaggcgtc cgcgaaatcg tacggcgtca
     4501 cggagtccac agagatgcgg tagaagtcca ggtcggccac gttgaccgga ttccaagcca
     4561 actggttctg gcctgtcccc ggcgtcgcgg tcaggttggc gggcgcggga ggcgcggtgt
     4621 cgtaggcgat ggcgaagtta ttgtcgttga tgccgtcgcc ggccgatccg cgggaccaag
     4681 tcccttggag ggcgatcatg ttctgcgcct tgatggccca gtagtaggtg gctccgggtt
     4741 cgaggcccgt gatgacttcg gcccgattgt tgcccggcga actcggcacc caggtttggg
     4801 tatacacggt ggccgcggcg aagtccgcat cgtcttcgat gttgggagtt gtttggtatc
     4861 ggacatggta acttgtcgcc ggaatgcctg aaccggctcc ggattcgggt tcggtccagt
     4921 tcagcgtgag ggccccttcg cggttgggat cgaacaacag gtccccttcc agtgccgtca
     4981 ggttggtgat ggccgcgggc gggttttgga cgacggaaac cgacagagtc atcgagtcgg
     5041 tcgtggccgc ttccacggag gccatgcctc cgaagaaccg ggaccggatc aacggctcaa
     5101 aaggcgcaaa ggtggcctgc gtcaccacaa gccacgcggt ggcgaggcgc aaccaaagcc
     5161 tcatatgttc acgccctttc ggtttcactt tcagcctctc gtcaatcttc gacgtccctt
     5221 tccgtttcag tcatttgctc acttctccgt gttcacgtag atgcgggcga tctggttcaa
     5281 tatcgccgtt tcgcccggca actccacttg aagcaggaac acgtaacgct tgccgcggta
     5341 ttcctcctgt tttggcatct ccaggaagac atggaacggc tcgatggtgt tcgcgtccac
     5401 gcgcagttct ttcttcggaa ggatgatgta actcggatcc ggaggagcct ccacgtcctt
     5461 ccccaccggc atgctgaggt ctctcggaga gacgctcgac aggtagta
//
LOCUS       DAOTFD010000178         5501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10642_length_5501_cov_3.846676, whole genome shotgun sequence.
ACCESSION   DAOTFD010000178 DAOTFD010000000
VERSION     DAOTFD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_5501_cov_3.846676"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="P5079_09165"
     CDS             <1..1773
                     /locus_tag="P5079_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY30189.1"
                     /translation="MGSGGMVVMDEDNCMVDVARYFTEFTTSESCGKCTPCREGLNQS
                     LESLISVTKGLAEDKDLAGLQTLGEVIKDTALCGLGQTAPNPVLTTLKYFKDEYEDHI
                     HAKHCKAGVCNTLFLAPCENSCPLKINIPGFLTLFRDNRLEEAFELVLRENPLPASTG
                     RICHFHCRTRCRRRDLDEPISSGEAHRHIADEIYKRKKEKGILQKIIQEKLPSTGKKV
                     AVIGAGPSGLMAAFYLLRLGHDVVIYEAHAEAGGILRYGIPEYRLPKAVLAKEIDFIR
                     QLGAKFVFNKNVTPAEFAKIEKDFDAVYLALGAYQSVPPGIPGEDLSGVWYGTHFLEE
                     VSSGRRPRIGKKAVVVGGGNVALDAARTLWRLGSEVTIVYRREKADMPADENEIKEAE
                     EEGIKFVLFAAPQSVAGDAHRHVRALEVTKMKAGEYDLSGRRRPEKTAETVPIPCDTL
                     VFAIGEKVESGFLEKCGLPVRKNGTLLVDRYSLRTNRRKVFGGGDMVMGPATAVEAMF
                     DGKNAAEKIDEFLTGKDRRAALSKRFAYAQTVPATPSRQKRQTAKELFVPARRGNFKE
                     VSLGLSPAQAMAESCRCLRCDVKV"
     gene            1778..3559
                     /locus_tag="P5079_09170"
     CDS             1778..3559
                     /locus_tag="P5079_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY30190.1"
                     /translation="MTDELITLKIDGKEVQVAEGTTLLKACKQAGVDVPSLCYLDGIN
                     EEASCSVCMVEVKNARTLVRSCVTKATNGAEVSTNTERVRRARRINVELLLANHPQDC
                     FVCDRNETCELRRLAHNLGVRQVRFPKTSGPRWELDTSSPSLVRDPNKCVLCRRCISV
                     CNRVQTVNAIDATQRGRRTVVSTFMDMGLGSVSCVNCGQCLLVCPTGAIVETNATAGV
                     WQALSDPEKTVLVEVAPAVRASIGEEFGLPPGTRVTGKMVAALRKLGFNKVFDTQFTA
                     DLTIMEEGHELIERLTHKGVLPMITSCSPGWIKFAEHFYPRLMKNISSCKSPQQMFGA
                     VAKTYYAQKLGLDPRRVTVVSIMPCTAKKYEAKRPEMVAAFHFWKDKLHLKESDVFPD
                     VDYVLTTREAARMMKEAGIKFVDLPEETFDSPLGMSTGAAVIFGATGGVMEAALRTAY
                     EVVTKKKLERLDFGDLRGLKGIKTTEVDLNGTKIKVAVANSLGAARKLLDEIEEGKSP
                     YTFIEIMTCPGGCLGGGGQPIPTDTEVREKRKMAIYEEDRNMPIRKSHENPHVQQLYK
                     EFFGEPLSDKAHHLLHTAYAARPLYRR"
     gene            3679..3930
                     /locus_tag="P5079_09175"
     CDS             3679..3930
                     /locus_tag="P5079_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRY30191.1"
                     /translation="MEKCLGFVGIILEDRKKSAAAVNKVLSESGEQIVARMGLPHVRG
                     NHAVITLIVDATKDEVASLTARLGALPGLTVKSASAEVR"
     gene            3986..4480
                     /locus_tag="P5079_09180"
     CDS             3986..4480
                     /locus_tag="P5079_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY30192.1"
                     /translation="MDIEKTDIEDIRRNVEEICAPWEGRPGNLIMILHQIQNRYSYVP
                     RRISFEVAKRLSIPLARIYEVITFYNYFKLEPPGKNIVSVCMGTACYLKGAPSLVERL
                     GQELGIKPGESTRDKNFYLQVVRCLGCCGLAPVLTVGQKVYSNVRPEQVPGILAEYRQ
                     GAVV"
     gene            4477..>5501
                     /locus_tag="P5079_09185"
     CDS             4477..>5501
                     /locus_tag="P5079_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30193.1"
                     /translation="MNRDELKKMRDEASAREKKTWIRVGMATCGVAAGAQETFFALRE
                     ELKKQKLAVELKKCGCVGRCSAEPLVEVAVEGMPTVFYRHLTKDKVPQLVESHFLKKT
                     VLRDQAVPLEDPKQLRIVLRNCGVVDPDSIRDYIARDGYAALAKVLAEMTPEQVIEEV
                     KKSGLRGRGGAGFPTGLKWDLTRKVPSDQKYVICNGDEGDPGAYMDRGVLEGDPHSVI
                     EGMMIAGYAIGADKGFFYIRAEYPLAVERIEKAIKQCHANKLLGQNIMGSAFSFDLEV
                     RLGAGAFVCGEETALIASIEGRRGYPHPRPPYPSVKGLWDKPTCINNVETLANLPPIL
                     AKGAAWFS"
BASE COUNT         1154 a         1507 c         1834 g         1006 t
ORIGIN      
        1 atggggtccg ggggcatggt ggtgatggac gaagacaact gcatggtgga tgtggcccgc
       61 tattttacgg agttcacgac gtccgaatcg tgcggaaaat gcaccccctg ccgggaaggc
      121 ttgaaccagt ccctggaaag cctcatctcg gtgacgaagg gcctggcgga ggacaaggat
      181 ctggcgggac tgcagacctt gggcgaggtc atcaaggaca cggccctgtg cggcctgggg
      241 caaacggcgc ccaaccccgt tctcacgacg cttaaatatt tcaaggacga atacgaggac
      301 cacatccacg cgaaacactg caaggcgggc gtctgcaaca cgttgttttt ggccccgtgt
      361 gaaaacagct gtcctctcaa aataaatatc ccgggcttct tgacgctgtt ccgggacaac
      421 cggctggagg aggccttcga gctggtgttg agggaaaacc cgctccccgc ttccacagga
      481 cggatatgtc atttccattg ccggacgcgc tgtcgccggc gggacctcga cgagccgatc
      541 tcgtccggag aggcccatcg gcacatcgcc gacgaaatct ataagaggaa gaaggagaag
      601 ggcatcctcc aaaaaataat ccaggaaaaa ttgccctcca cgggcaagaa agtggcggtc
      661 atcggcgccg gccccagcgg cctgatggcg gcgttttacc tgctgcggct cgggcatgac
      721 gtcgtcattt acgaggccca cgccgaggcg ggcggcatcc ttcgttacgg gatccccgaa
      781 tatcgcttgc cgaaagcggt tttggccaag gagatcgact tcatcaggca gttgggggcc
      841 aagttcgtct tcaacaagaa cgtcactcca gcggagttcg ccaagatcga gaaggatttc
      901 gacgccgttt acctggccct gggggcctac cagagcgtcc ctcccggcat tcccggcgag
      961 gacctgtcgg gcgtttggta cgggacccat ttcctcgagg aggtttcttc ggggcgtcgg
     1021 cccaggatcg ggaaaaaggc ggtggtggtc ggcggaggga acgtggcgct ggatgccgcg
     1081 cggactttgt ggcgtttggg atcggaagtg accatcgtct atcggcgtga aaaggccgat
     1141 atgcccgccg acgagaatga aatcaaagag gcggaggagg aagggatcaa gttcgttctt
     1201 tttgccgcgc cgcagtccgt ggcgggggac gcgcacaggc atgtccgggc gttggaagtc
     1261 acgaaaatga aggcgggcga atacgacctg tcgggacgga ggcgtccgga gaagaccgct
     1321 gagaccgtgc cgatcccctg cgacacgctc gttttcgcca tcggtgaaaa agtggagagc
     1381 ggtttccttg aaaaatgcgg actcccggtg aggaagaacg ggacgctgct tgtggatcga
     1441 tattctctcc ggacgaaccg gcggaaggtg ttcggtgggg gcgacatggt catggggccg
     1501 gcgacggccg tggaggccat gttcgacggc aagaacgccg ccgagaagat cgacgaattt
     1561 ttgacgggga aggaccggcg ggcggccctt tcgaaacggt tcgcctatgc ccagaccgtc
     1621 ccggcgacgc cgtcccgcca gaagaggcag acggcgaaag agctgttcgt cccggcgagg
     1681 cgcggaaatt tcaaggaagt gtccctggga ctgtcgccgg cgcaggcgat ggccgagtcg
     1741 tgccgttgct tgagatgcga cgtgaaggtg tgaaatcatg accgatgaac tgatcaccct
     1801 gaaaatcgac ggcaaagagg tgcaggtcgc ggaggggacc acccttctga aggcctgcaa
     1861 gcaggccggg gtcgacgttc cttccctgtg ttacctggac ggcatcaacg aggaggcctc
     1921 gtgttcggtc tgcatggtgg aggtcaagaa cgcccggacg ctcgtccggt cgtgcgtgac
     1981 caaggccacc aacggggccg aggtctcgac gaacacggag agggtgcgcc gggcgcgccg
     2041 gatcaacgtg gaactgcttt tggccaacca cccgcaggac tgtttcgtct gcgacaggaa
     2101 cgagacatgc gaactgcgga gactggcgca caacctgggg gtccggcagg tccgtttccc
     2161 caaaacgtcc ggtccccggt gggaactcga cacctcctct ccgtcgctgg tgcgggaccc
     2221 gaacaaatgc gtcctttgcc ggcggtgcat ttccgtgtgc aaccgggtgc agacggtcaa
     2281 cgccatcgac gcgacccagc gcggccgccg cacggtcgtt tccacgttca tggacatggg
     2341 gctgggatcc gtcagctgcg tgaattgcgg gcaatgtctt ttggtctgcc cgacgggagc
     2401 gatcgtcgaa accaacgcta ccgccggcgt gtggcaggct ctgtccgatc cggaaaagac
     2461 cgtgctggtg gaagtggccc cggccgtccg ggccagcatc ggggaagagt tcggtctgcc
     2521 cccggggacc cgcgtcacgg ggaagatggt ggcggccttg aggaaactgg ggttcaacaa
     2581 ggtgttcgat acgcagttca ccgccgatct gaccatcatg gaagaagggc atgagctcat
     2641 cgagaggctc acgcacaagg gggtcctgcc catgatcacg tcctgttccc cgggatggat
     2701 caagttcgcg gaacattttt atccgcggct gatgaagaac atttcgagtt gcaaatcgcc
     2761 tcagcagatg ttcggggccg tggccaagac ctattacgcc cagaaattgg gcttggaccc
     2821 ccgccgggtg acggtggttt ccatcatgcc ctgcacggcc aaaaaatacg aggccaaacg
     2881 gccggagatg gtcgcggcgt tccatttttg gaaggacaag ctgcatttga aggagtcgga
     2941 cgtctttccg gacgtcgatt acgtcctcac cacgcgcgaa gcggcccgga tgatgaaaga
     3001 ggccggtatc aagttcgtcg atctgccgga ggagacgttc gacagccccc tggggatgtc
     3061 gacgggggcg gcggtcatct tcggcgcgac gggcggcgtg atggaggccg cgttgcgcac
     3121 ggcctacgag gtggtgacca agaaaaaact cgagcgtctc gatttcggcg atttgagggg
     3181 attgaagggc atcaagacga cggaagtcga cctgaacggc acgaaaataa aagtggccgt
     3241 tgccaacagc ctgggcgccg cccgcaagct tctggacgag atcgaggagg ggaagtcccc
     3301 ctatactttt atcgagatca tgacctgccc cgggggatgt ttgggcggcg gcggacagcc
     3361 gatccccacg gacaccgagg tccgtgaaaa aaggaaaatg gccatttacg aggaagaccg
     3421 gaacatgccg atccgtaaat cccacgagaa cccccacgtg cagcagttgt ataaggaatt
     3481 ttttggagaa cctctgagcg acaaggcgca tcatctgctg cataccgcct acgcggcccg
     3541 cccgctgtat cggcgataga ccgaaccgat acgttggcgg ggccaagggg acgcgtcttt
     3601 tcttaacgag aaataaagcg acccgatttt catttccacg gaaatggaaa tcggtttttt
     3661 atcggcgagg aggagatcat ggagaaatgt ctgggtttcg tggggatcat tctggaagac
     3721 agaaagaaat cggccgccgc cgtcaataag gtgctttcgg agtccggcga acagatcgtg
     3781 gcgcgcatgg ggctgccgca cgttcgggga aaccacgcgg tgatcacgct catcgtggac
     3841 gccacgaaag acgaggtggc gtccttgacc gcccgtttgg gcgcccttcc ggggcttacc
     3901 gtgaagtccg cctccgccga ggtgcgctga acccgtccgg cttcgggacg tcggcgtgaa
     3961 acagggatag gaagaagaca tccgaatgga tattgaaaag accgacatcg aggatatccg
     4021 ccggaacgtg gaggagatct gtgcgccttg ggaggggcgc ccgggcaatc tcatcatgat
     4081 cctccatcag atacagaacc gctacagcta cgttccccgt cggatatcct tcgaggtcgc
     4141 caaacggttg tccatccccc tggcccgaat ttacgaggtc atcaccttct ataactattt
     4201 caagctcgag ccgcccggca agaacatcgt ttccgtctgc atggggaccg cctgctattt
     4261 gaagggggct ccgtcgctgg tggagaggct ggggcaggag ttggggatta agccgggaga
     4321 gtccacccgg gacaagaatt tttatctgca ggtcgtgcgt tgcctgggat gttgcggcct
     4381 ggccccggtg ctcacggtgg ggcagaaggt ttacagcaat gtccgtccgg aacaggtgcc
     4441 cggcattctg gcggaataca gacaaggggc ggtcgtgtga accgggacga gctgaaaaaa
     4501 atgcgggacg aggcctccgc gcgggaaaag aaaacctgga tccgcgtcgg catggccacc
     4561 tgcggggtcg ccgccggcgc gcaggagacg ttttttgcgt tgcgggagga actgaaaaaa
     4621 caaaaactgg ccgtggaact caagaagtgc ggatgcgtgg gacgatgttc ggcggagccg
     4681 ttggtggaag tcgcggtgga ggggatgccc acggtgtttt atcggcacct caccaaggac
     4741 aaggtccctc aactggtgga gtcgcatttt ttgaagaaga ccgttttgcg ggatcaggcg
     4801 gtccctctgg aggaccccaa acaactccgc atcgttcttc ggaactgcgg cgtggtggac
     4861 cccgacagca tccgggatta catcgcccgg gacgggtacg cggcgttggc gaaggtgttg
     4921 gcggagatga cgccggaaca ggtgatcgag gaggtcaaaa agagcggtct gcgcggccgc
     4981 ggcggcgcgg gttttccgac cggcttgaag tgggatttga cgcggaaggt cccttccgat
     5041 cagaaatacg tcatctgcaa cggcgacgag ggcgatcccg gcgcgtacat ggaccgcggc
     5101 gtgttggagg gggacccgca ttcggtgatc gaaggcatga tgatcgcggg ttacgccatc
     5161 ggcgccgaca aaggtttttt ttacatccgc gcggaatatc cgttggcggt ggagcgcatc
     5221 gagaaggcca tcaaacagtg ccacgccaac aaactgttgg ggcagaacat catggggtcc
     5281 gccttttcgt tcgacctgga ggtgcggctc ggggcgggag ctttcgtgtg cggcgaggaa
     5341 acggccctga tcgcttccat cgaagggcgc cgcggatatc cgcatccccg tccgccgtat
     5401 ccgtcggtca aggggctttg ggacaagccc acctgcatca acaacgtgga gaccctggcc
     5461 aacctgccgc ccatcctcgc gaagggggcc gcgtggtttt c
//
LOCUS       DAOTFD010000179         5465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10763_length_5465_cov_4.397043, whole genome shotgun sequence.
ACCESSION   DAOTFD010000179 DAOTFD010000000
VERSION     DAOTFD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10763_length_5465_cov_4.397043"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="P5079_09190"
     CDS             complement(<1..737)
                     /locus_tag="P5079_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY30194.1"
                     /translation="MNRPTRWQILLNAYTPEKRNWDRIYPWLAYVLRPLSFPVAWALM
                     PFGVTANHVTFVTLLLGLGTCAAFAAGRPVTGAVLLILLNLFDCVDGDLARAQPPAKL
                     PVGKFYDQLVGNVWLSVYFFMGAGFLTGDGETFLLPSRMAVLGGATTLLKCLSLHTRQ
                     IFWNVLGPAWESQKSADPSKPKAHSTSWYYRLYYNVTDIQAQDPLVLFFALVGRLDVF
                     LALSFLALADEFVFVTLLCLRRARRMAG"
     gene            complement(734..2398)
                     /locus_tag="P5079_09195"
     CDS             complement(734..2398)
                     /locus_tag="P5079_09195"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY30195.1"
                     /translation="MTQDRPYLPDTPETLDDFYRQEDPWKTRSLYEKKIRYRFLKRLV
                     PLRLKKTLELACGEGDFTSFLRTRSTQVTAYDLSAVALDRARRKFPGISFETRDARAV
                     GVDDFARHDAVVWLDALNFMPHSESAQTLSRLAQSAESSGKFFLFSTRILPETRTDMS
                     YRPGYDFTTPQSFTRHVAEHFPNARSVPVQLHFDLRPAGDLSLLQKLAKLKMKILTKG
                     GLYPLVLFFVQKTWGLPFYQRLLSPFVIHLAITAESPVYYDKRLSEDVTDRLINRPAA
                     AMLVTRLIPTAITPNQITAVSFLLGIAAALFIATGGGAASAVGALLLELSIVVDCVDG
                     QLARAKGLTSEWGDIFDHTSDDATLLLICAAAGWALRHTVPAAGLVLLCLLAFSAVLH
                     LTIAQYFYSAEYASLVGQGAPEKIRTDRRRVQQMDADRPWSAASLFPKLLLRYYAFRL
                     WVIRAHTLFFNPARKKFTARPAEDRARRIVYHERQRGVLEWWKYGGVSSIALAFVVFC
                     LAHRLLALLLLLISAGHLFFCLLLIRQKKADRRYLEDISKTGDNRP"
     gene            complement(2395..3357)
                     /locus_tag="P5079_09200"
     CDS             complement(2395..3357)
                     /locus_tag="P5079_09200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30196.1"
                     /translation="MSPTTVSVNVLSWNGRDWLRPCLDSVLASLDAPGLNGKVLVIDN
                     ASCDGTRLVLDAYKGRIETLLLSENHGFTGGHNRGIRRALQQGAEWVALLNQDVRVEK
                     DWLLRLLEVAGKNPRAGILSPFQYDYDGLDLDPGFRRTLSLLPETAEGLISHRWNAPY
                     YVSDMAAGAALLIHRRVFEKIGVFDPAYFSYHEESDLYRRAMAAGAEIVLVPKSRIFH
                     WHTLRRRPLPAWLALINTRNYFRIILKNPNAPFAKNLLEAARTWWKCLRDSLTAKKHR
                     RTALLLWTGLADLPPRIPFIYWRRAAERWMIRAAGNNFSNRGNP"
     gene            complement(3332..4495)
                     /locus_tag="P5079_09205"
     CDS             complement(3332..4495)
                     /locus_tag="P5079_09205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY30197.1"
                     /translation="MRIHFFYYEFDPRVRGGMGGFAHVWQLAKNWRSLGHDVWVFSPA
                     TGPADEQSPTRLVRIPVIPLPYLRPLSAYLLSFLFGLFSSRTHRPDLIYAREMFSPLL
                     VLLARCSGCPLFLEINGDSFADHRRRGASALRRRWVEAIQSFNFTRAAKVFAVTEGLR
                     RTLIERHGLPGEKVVALPNGADTDLFFPRAAAECRKILSLPARGPVVGFVGTFYEYQG
                     LDILIQAAPAVLKKFPDALFLLAGDGVERAAWEDLAGRAGLKSRFLFTGQVPYEKVPL
                     YLNAMDVAVAPFRSFRGETSPLKIFDAWACAKAVVASDIPALASLKTGEEKWVAVPPD
                     DPAALAAALNALLSDPDRRERLGRAGFRHIQNGHRWQDVAREIIEHVAHDRLR"
     gene            complement(4492..>5465)
                     /locus_tag="P5079_09210"
     CDS             complement(4492..>5465)
                     /locus_tag="P5079_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY30198.1"
                     /translation="YFFLYILVSNLLTDKKQLKAFLWAGVVTALIVGVFAYAHIGKVT
                     RLYAPFDESIGAGTGESATLGGYLLIILCLSMTFFTQIPHLHAAFAGLALFVFLLPPF
                     VLTLSRASYVGLFAGLLTIVFFTRRRKILLLSLLAVAAAGAPIVSPGLYGAAQRRLQE
                     TFLTPASHLQVSGWNVGLETSAAQRINSWRYAFSLWLPKHPLLGHGVTGVGLVDAQYP
                     LILGETGVLGFVAFGWLLWTLLKHTHRIYRRSQSGLDRGLSLGLLAACAGLCTQAFTT
                     NTFIIVRIMEPFWFLAAVVMRLPSLSEPSVPATEPAGPPSLVPVGRP"
BASE COUNT         1085 a         1523 c         1976 g          881 t
ORIGIN      
        1 ccggccatcc gccgcgcccg tcggagacag aggagggtga cgaacacgaa ctcgtcggcc
       61 agagccagga aactcaacgc caggaaaacg tccaggcggc cgaccaaggc gaaaaacagg
      121 acgagcggat cttgggcctg aatgtcggtg acgttgtaat agaggcggta ataccaggag
      181 gtggagtggg ctttcggctt cgaggggtcc gcggattttt gcgattccca cgccggaccc
      241 agcacgttcc agaatatctg gcgggtgtgc agactcaggc atttaagcag ggtcgtcgcc
      301 ccgcccagaa cggccatccg ggacggaagg aggaaggtct ctccgtctcc cgtcagaaat
      361 cccgcgccca tgaagaaata aacggagagc catacgttgc ccacgagttg gtcgtagaat
      421 tttccgacgg ggagcttcgc cggcggctgg gcgcgggcca ggtcgccgtc cacgcagtcg
      481 aagaggttga ggaggatgag gaggacggcc cccgtcacgg ggcggccggc ggcgaaggcc
      541 gcgcaggtgc cgagccctag gagcagggtg acgaacgtca catggttggc cgtcacgccg
      601 aaaggcatga gggcccaggc gacgggaaat gacagcggcc ggaggacgta cgccagccag
      661 gggtagatcc tgtcccagtt ccttttttcg ggcgtgtagg cgttgagcag gatctgccag
      721 cgcgtgggcc tgttcatggg cggttgtccc ccgtttttga aatgtcttcc agatatcgcc
      781 ggtcggcttt tttctgtcgg atcagcagga gacagaaaaa gaggtgtccg gcggagatca
      841 ggagcagcaa aagggccagg aggcggtggg cgagacagaa gacgacgaag gcgagggcga
      901 tggagctgac gccgccgtat ttccaccact ccaggacccc gcgttgccgc tcgtggtaga
      961 cgatgcgccg cgcccggtct tcggcggggc gggcggtgaa ttttttccgg gcaggattaa
     1021 aaaagagcgt gtgcgcccgg attacccaga ggcgaaaagc gtagtagcgg aggaggagtt
     1081 tggggaacaa ggaggccgcg ctccagggcc ggtcggcgtc catttgttgc acgcggcgcc
     1141 ggtctgtgcg gatcttctcc ggcgcgccct gccccacgag ggaggcgtat tccgcggaat
     1201 aaaagtattg cgcaatggtg aggtggagca ccgcggagaa cgccaggagg caaaggagga
     1261 cgagccccgc ggccggcacg gtgtgccgga gggcccagcc cgcggcggcg cagatgagca
     1321 ggagcgtggc gtcgtccgag gtgtgatcga agatgtcccc ccattcggag gtgagccctt
     1381 tggcgcgggc gagttgtccg tcgacgcagt ccaccacgat ggagagctcg agcaggaggg
     1441 cgccgacggc ggaggcggcc ccgccgccgg tcgcaatgaa cagcgccgcc gcgatgccca
     1501 ggagaaaaga caccgcggtg atctggttgg gcgtgatggc ggtggggatg aggcgcgtca
     1561 ccagcatcgc cgccgccggc cggttgatga gccggtcggt cacgtcttcc gagagccgtt
     1621 tgtcgtaata gaccggggat tccgccgtga tcgccaggtg gatgacgaac ggcgaaagga
     1681 ggcgttgata aaaagggagg ccccaggttt tttggacgaa aaacaggacg aggggataga
     1741 gccccccctt cgtgaggatt ttcatcttca gtttcgccag tttctggagg agcgagaggt
     1801 cgccggccgg acggagatcg aaatgcagct gcaccgggac ggatcgggcg ttggggaagt
     1861 gctcggcgac gtgccgggtg aagctctgcg gcgtcgtgaa atcgtatccc ggccggtagg
     1921 acatgtccgt ccgcgtttcc ggcaggatgc gggtggagaa caggaagaat tttccggaag
     1981 actcggcgct ctgcgccaag cgggagagtg tctgcgcgct ctccgaatgc ggcatgaagt
     2041 tgagggcgtc cagccagacg acggcgtcgt gtcgggcgaa atcgtcgacg ccgacggcgc
     2101 gcgcgtcccg cgtttcgaag gagatgccgg gaaatttccg ccgcgcccgg tccagcgcta
     2161 cggcggagag gtcgtaggcg gtgacctgag tcgagcgcgt tcggaggaag gaggtgaaat
     2221 ccccctcccc gcaggccagc tccagcgttt ttttcaggcg caggggaacg aggcgtttca
     2281 agaacctata ccggattttt ttctcataca acgaacgcgt cttccagggg tcctcctggc
     2341 ggtagaaatc gtccaaggtt tcgggagtgt cggggagata ggggcggtct tgggtcatgg
     2401 gttcccccgg ttcgagaaat tatttcccgc ggcgcggatc atccaacgtt ccgccgcgcg
     2461 ccgccaataa atgaaaggga tcctcggcgg caggtccgcc aggccggtcc acaggagcag
     2521 ggccgtgcgg cggtgttttt tcgccgtcag ggagtcgcgg aggcatttcc accaggttcg
     2581 ggcggcttcg agcaggtttt tcgcaaaagg ggcgttcggg ttcttcagga tgatgcggaa
     2641 ataattccgg gtgttgatga gggcgagcca ggccggcaac ggccggcggc gcagggtgtg
     2701 ccagtggaag atgcggcttt tcggcaccag aacgatctcc gccccggcgg ccatggctcg
     2761 gcggtagagg tcggattctt cgtggtagct gaaataggcc gggtcgaaca cgccgatttt
     2821 ttcgaaaacc cggcggtgga tcaggagggc cgctccggcg gccatgtcgc tgacatagta
     2881 gggggcgttc caccgatggg agatgagtcc ctcggcggtt tccggcagga ggctcaatgt
     2941 ccgacggaag ccggggtcca ggtccaaccc gtcgtaatcg tattggaacg gggaaaggat
     3001 ccccgcgcgg ggattctttc cggccacttc caggagccgc aggagccagt ctttttccac
     3061 gcgcacgtcc tggttcaaca gggcgaccca ctcggcgccc tgttgaagag cccgccggat
     3121 gccgcggttg tgtccgccgg tgaacccatg gttctccgaa aggagaaggg tctcgatgcg
     3181 gcctttgtag gcgtcgagca cgagcctcgt gccgtcgcag gaagcgttgt cgatgaccag
     3241 gactttcccg ttgagcccgg gagcgtcgag ggacgccagc accgagtcga ggcacggcct
     3301 cagccagtcc cggccgttcc aggaaaggac gttaacggag acggtcgtgg gcgacatgct
     3361 cgatgatttc ccgggccacg tcctgccagc gatggccgtt ttggatatga cggaaccccg
     3421 cccggcccag ccgttcccgt cggtcgggat cggacaaaag ggcgttgagg gcggcggcca
     3481 gggcggcggg gtcgtccggc gggacggcga cccatttttc ttcgccggtt ttcaaggagg
     3541 ccagggcggg gatgtccgag gcgacgaccg ccttggcgca ggcccaggcg tcgaatatct
     3601 tcaacggcga cgtttccccc cggaacgacc ggaacggcgc caccgcgacg tccatggcgt
     3661 tcaggtagag aggcactttt tcatagggga cttggcccgt gaaaagaaaa cgtgatttca
     3721 gtccggcccg cccggcgaga tcttcccagg cggcgcgttc gacgccgtct cccgccagaa
     3781 ggaaaagggc gtccgggaat tttttcagga ccgccggcgc cgcctggatg aggatgtcca
     3841 gcccttggta ttcgtaaaag gtgccgacga acccgacgac gggcccccgg gccggcaggg
     3901 agaggatttt ccggcattcg gccgccgccc gggggaaaaa aaggtccgtg tccgcgccgt
     3961 tgggaagggc gaccactttc tcgccgggca gtccgtggcg ttcgatgagg gtgcgacgca
     4021 atccttcggt gacggcgaag accttcgccg cccgcgtgaa attgaaggac tggatcgctt
     4081 caacccatcg ccgccggagg gcggaggcgc cgcgccgtcg atggtctgcg aagctgtcgc
     4141 cgtttatctc caaaaacagc ggacagcccg agcatcgggc gagcaggaca aggaggggtg
     4201 agaacatttc ccgggcgtaa atcaggtccg gccggtgtgt ccgggaagaa aaaagaccga
     4261 acaggaacga aagcaggtag gcgctgagcg gccgcagata gggcagcggg atcaccggga
     4321 tgcgtaccag gcgcgtcggc gattgttcgt cggcgggacc ggtggccggc gaaaagaccc
     4381 aaacgtcgtg ccccagggaa cgccaattct tcgccagctg ccagacgtgc gcaaatccgc
     4441 ccatcccccc ccgcactcgg ggatcgaatt cgtagtagaa gaaatggatc ctcacgggcg
     4501 tccgaccgga acgagcgatg gcggaccggc gggttccgtc gccgggaccg acggctccga
     4561 aaggctcggg aggcgcatca ccacggccgc gaggaaccaa aacggttcca tgatgcggac
     4621 gatgatgaag gtgttggtgg tgaaggcctg cgtgcacagc cccgcgcagg cggccagaag
     4681 gcccagcgag agcccgcggt cgagaccgga ctgggaacgt cggtaaatgc ggtgggtgtg
     4741 tttgaggagc gtccagagaa gccaaccgaa agccacaaaa ccgaggacgc cggtttctcc
     4801 gaggataaga ggatactggg cgtccaccag tcccacgccc gtcacgccgt gtcccaggag
     4861 aggatgtttc ggcagccaga gggagaaggc gtaacgccag ctgttgatgc gttgcgccgc
     4921 ggaggtctcc agcccgacgt tccagccgga gacctggagg tgggacgcgg gggtcaggaa
     4981 ggtttcctgg agccgccgtt gggcggcgcc gtagagaccc ggggagacga tgggggcccc
     5041 ggcggccgcc acggccaaaa gggagaggag gagtattttc cgtcgacgcg tgaagaacac
     5101 gatggtcaga aggccggcga aaagaccgac gtaggaggcc cgcgacagcg tgaggacgaa
     5161 aggcggcaag aggaacacga agagggccaa accggcgaaa gcggcgtgca ggtgcgggat
     5221 ttgggtgaag aacgtcatgg agagacagag gatgatgagg aggtagccgc ccagggtggc
     5281 cgactcgccg gtgccggcgc cgatgctctc gtcgaacggc gcgtacaggc gggtgacttt
     5341 tccgatatgg gcgtaggcga acacgccgac gatgagggcg gtgacgacgc ccgcccacag
     5401 gaaagccttg agctgttttt tatcggtcag caggttggag accaggatat agagaaaaaa
     5461 atatt
//
LOCUS       DAOTFD010000180         5457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10791_length_5457_cov_4.110515, whole genome shotgun sequence.
ACCESSION   DAOTFD010000180 DAOTFD010000000
VERSION     DAOTFD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_5457_cov_4.110515"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5079_09215"
     CDS             complement(<1..511)
                     /locus_tag="P5079_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY30199.1"
                     /translation="MKHLVTGGGGFLGSHIARRLASGGSAVRLMDIREPRSPGPGTEF
                     IRASILDRNSVREALRGVDVVHHTASLVPVTKAGGNFFEVNVTGSRILAEEAVRAGVR
                     CFVHMSSTAVFGAPERVPLEEDSPMRPVEIFGRTKMEGEAVVGDICRRNGVPLIVLRP
                     RTVLGKGRMG"
     gene            complement(508..1575)
                     /locus_tag="P5079_09220"
     CDS             complement(508..1575)
                     /locus_tag="P5079_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY30200.1"
                     /translation="MKLDGSLILLTGALGWAGRGFLNAIFHGLPDCDPFRKPLNCRLR
                     CFVEPGQDLSVMGGSSREVENIVGDLRDPSDCDRFCAGARDAVLFHVAGVVHPRNVAE
                     FYSVNVDGTKNLLAAAARAGVRRVVAVSSNSPCGYNTSEGHFFDEEIPYRPYRHYGRS
                     KMEMEKVVRSFYDAGRMETVIIRPAWFYGPRQPPRQTLFFRMIQKGLVPLIGNGLNLR
                     SMTYIDNLCQGLLLAADSPHAAGQTFWIVDERAYRMEEIISTVERLLENEFHVACEHR
                     RIRLPGVVADMAGVLDAAFQSFGFYNQKIHVLSEMNKTIACSMRKAREVLGYRPTIAL
                     EEGMRRSLAWVFQAQGKRKLL"
     gene            2067..2705
                     /locus_tag="P5079_09225"
     CDS             2067..2705
                     /locus_tag="P5079_09225"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HRY30201.1"
                     /translation="MRCSRVCVLVSGGMDSAVLLSEALRRFRAVQPLYIRCGLRWERA
                     ELFHLRKFLSSLRPPRPSPLSIVEVTTRDIYGPHWSLSGPDVPGYRSRDEKTYLPGRN
                     ILLLSKAAVFCALRGLPRLWLGPLRGNPFPDARPAFLKALSVALRKGLDFPITIQAPF
                     AGLRKRDIVVRGRDLPLHLTFSCLNPRGLRPCGQCNKCAERDRGLKFFHPSK"
     gene            complement(2760..3449)
                     /gene="rplA"
                     /locus_tag="P5079_09230"
     CDS             complement(2760..3449)
                     /gene="rplA"
                     /locus_tag="P5079_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRY30202.1"
                     /translation="MSRRLLESKKLLEKGKKYSLAEAVAVLKKSATAKFDETVEVHIR
                     LGIDPKKSDQNVRGNVVLPHGLGRSKRVVVVAKGEKVKEAQAAGADEAGDAELVEKIS
                     KGWLDFDVLVATPDAMKDLSKLGKVLGPKGLMPNPKSGTVTFDLARIVKELKAGRIEF
                     KADDYGIIHGILGKASFPEKNIVENADALLNAVQAARPNTAKGPYIRTITLCTTMGPG
                     VGVQPEVVATK"
     gene            complement(3478..3906)
                     /gene="rplK"
                     /locus_tag="P5079_09235"
     CDS             complement(3478..3906)
                     /gene="rplK"
                     /locus_tag="P5079_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRY30203.1"
                     /translation="MAKKVKTQIKIQIPAGGANPAPPVGPALGQHGVNIMDFCKQFND
                     RTKSMEAGMVIPAVITVYDDRSFTFITKMPPVSALIKKAAGLAKASSEPNKTKVGKIT
                     RSQAEEVAKTKMPDLNANSLNAAVEMVKGTARSMGIEVDE"
     gene            complement(3931..4461)
                     /gene="nusG"
                     /locus_tag="P5079_09240"
     CDS             complement(3931..4461)
                     /gene="nusG"
                     /locus_tag="P5079_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY30204.1"
                     /translation="MGRAWYVIHTYSGHEDKVKSSLEKAVATQSLSGLINQVLIPTED
                     VVELKRNKKQVKKRKFFPGYILADMEINNETYWLVRNTAGVTGFLGGVKPVPLPDSEV
                     QHLLELVSAPSTTKPRPAVLFEKGENVRINDGPFRHFIGSVEDVNEDRAKLRVTVSIF
                     GRATPVELDYLQVEKL"
     gene            complement(4478..4657)
                     /gene="secE"
                     /locus_tag="P5079_09245"
     CDS             complement(4478..4657)
                     /gene="secE"
                     /locus_tag="P5079_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY30205.1"
                     /translation="MTDILRFFREAYDELKRVSWLSRTQMLASTWLVILLVIIFSIYV
                     GVVDYIIARMFGLLI"
     gene            complement(4847..4921)
                     /locus_tag="P5079_09250"
     tRNA            complement(4847..4921)
                     /locus_tag="P5079_09250"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4886..4888),aa:Trp,seq:cca)
     gene            complement(5153..5308)
                     /gene="rpmG"
                     /locus_tag="P5079_09255"
     CDS             complement(5153..5308)
                     /gene="rpmG"
                     /locus_tag="P5079_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY30206.1"
                     /translation="MAKERFVYMLACSECDNRNYHYVRGRRREKKLEMKKFCSSCGKH
                     TKHKETK"
BASE COUNT         1131 a         1755 c         1426 g         1145 t
ORIGIN      
        1 cgcccattct tcccttgccc aacaccgtgc ggggtcgcaa tacgattaaa gggaccccgt
       61 tcctccggca aatgtcaccg acgaccgctt ccccctccat cttggtacgc ccaaatattt
      121 ccacgggacg catggggctg tcttcctcaa gcgggacacg ctcgggcgcc ccgaaaacag
      181 ccgtggagct catgtggaca aaacaccgga cacccgcccg aacagcctcc tccgccaaaa
      241 tacgactccc tgtcacattg acttcaaaaa aattcccgcc tgcttttgtg accggcacca
      301 aagatgccgt gtgatggacg acatcaaccc cccgcagcgc ctccctgacc gaattccggt
      361 ccagaatgct tgcccgaatg aattcggttc ccggcccggg agagcggggc tcacgaatat
      421 ccataagacg aacagcgctg ccgcccgacg ccaaacgcct ggcgatatga ctccccaaaa
      481 aacctccgcc gccggtcacc agatgtttca taaaagtttc cgttttccct gggcctgaaa
      541 aacccaggcc aaactgcgcc gcattccctc ctcaagggcg atcgtcggtc gatatcccag
      601 aacctcgcgg gcctttcgca tcgaacaagc gatcgtctta ttcatctcgg ataatacgtg
      661 tatcttttga ttgtaaaatc caaaagattg aaaggcggcg tcaagcacgc cggccatatc
      721 cgcgacgacc ccgggcaaac gaattcgccg atgttcacat gcgacatgaa actcattttc
      781 caacaatcgt tccaccgtcg atattatttc ctccatgcga tacgcacgct cgtcgacaat
      841 ccaaaacgtt tgtccagcgg catgcggcga atccgccgcc agcaacaacc cctgacatag
      901 gttgtcaata taagtcattg acctgaggtt gagtccgttg ccgatgagcg gaacgagtcc
      961 tttctgaatc attcgaaaaa ataacgtttg gcggggcggt tgccggggac catagaacca
     1021 ggcgggccgg ataatgacag tctccattct tccagcatcg taaaaggacc ggacgacctt
     1081 ctccatttcc atcttggaac ggccatagtg ccgatagggc cgatacggga tttcttcgtc
     1141 gaaaaaatga ccctcgctcg tattgtagcc gcagggcgaa ttggaggaga ccgccacgac
     1201 gcgccggaca ccggcccggg ccgcggcggc gagaagattt ttagttccgt ccacgttgac
     1261 ggaataaaac tcggcgacat tccttggatg gacgacccct gcgacatgaa acaacacggc
     1321 atcgcgggcg ccggcgcaaa aacggtcgca gtcggaaggg tccctcaaat cgccgacgat
     1381 gttctccacc tcacgggacg aaccgcccat aacgcttaaa tcctgccccg gctcaacgaa
     1441 acacctcaaa cggcagttca acggttttcg gaatgggtcg caatccggaa ggccatgaaa
     1501 aattgcgttc aaaaaacctc ggcccgccca accgagggcg cctgtgagaa ggataagcga
     1561 accgtcaagc ttcatacagc gagcccgaac ggaaagcgcc tcaacccctt tgagacgtcc
     1621 ctgggggaca aggatcgccg aacgcatatt tcatgagcat ctgcgtcaaa aagttcccgc
     1681 atgcgatttt ccctcaattt aaacggactc cgggcaggtt gttcttttcg cgaccaacaa
     1741 gatcccgacg gaaatatccc cgcggggcag gcgtccctgc gttaccccac gccagacgat
     1801 gtgcgcgtcc gaaacgcgcc aaacgagtcc cactcgactt cgatgcgctc gcatccccga
     1861 agccgcccca aaaaccggac gccggaatcg gcaggggaca cgacggtcaa cgggatcccg
     1921 caggttaaac gcggccccgg ccgaatcgcc ccttgagagg aaagcataca tcttgataaa
     1981 aacgatatca acgaatcgac cgacatgcct cgcacgcggc accgaggcgt attcaacatg
     2041 cgtcgaaaaa ctgataaatt gttttcatga gatgttcccg cgtgtgcgtc ttggtcagcg
     2101 gcggaatgga cagcgccgtc ctcttaagcg aggccctccg gcgtttccgc gccgtccagc
     2161 cgctctatat ccgatgcggg ctgcgttggg aacgggccga actgtttcat cttcgaaaat
     2221 tcctctcctc cctccggccg ccgcgaccca gcccgctctc catcgttgag gtgaccacgc
     2281 gggacattta cgggccccac tggagtcttt ccggccccga tgtcccgggc tatcgctcac
     2341 gcgacgaaaa aacctatctc ccgggccgaa acatcctgtt gctctccaaa gccgcggtgt
     2401 tctgcgctct gcgcggcctt ccgcgccttt ggctgggacc gttgagagga aaccccttcc
     2461 cggacgcccg gccggctttt ctgaaagcat tgagcgtcgc cttgcggaaa ggcctggatt
     2521 ttcccatcac catccaagcc ccctttgccg ggctccgcaa gcgagatatc gtcgtccggg
     2581 gacgcgatct ccccctgcat ctgacgtttt cctgcctcaa tccccgaggg ctccggccct
     2641 gcggccaatg caacaagtgc gccgaacgcg accggggact gaagtttttc cacccttcca
     2701 aataaaaaag cccgtccttc cggacaggct ctttgcgaac tcgcaaccgc cgaaaaaacc
     2761 tacttcgtgg cgacgacctc cggctgaacc cccacgccgg gacccatggt ggtgcacaac
     2821 gtgatggtgc ggatgtaggg gcccttggcc gtattcggcc gggccgcctg aacggcgttg
     2881 aggagggcgt ccgcgttctc cacgatgttc ttctccggaa aagacgcctt ccccaggatc
     2941 ccgtgaatga tgccgtagtc atcggccttg aactcgatgc ggccggcctt cagttccttg
     3001 acgatgcggg ccaggtcgaa ggtcaccgtg ccgcttttcg gattgggcat cagccccttg
     3061 gggccgagca ccttgccgag cttggaaagg tccttcatgg cgtcgggcgt ggccacgagc
     3121 acgtcgaaat ccagccagcc cttggaaatt ttctccacca actcggcgtc ccccgcctcg
     3181 tcggccccgg cggcctgggc ctcctttact ttctcgcctt tggccaccac caccacgcgc
     3241 tttgagcggc cgaggccgtg cggcaggacc acgttcccgc gcacgttctg gtcggatttc
     3301 ttcggatcga tgccgaggcg gatatggact tccaccgtct catcgaactt ggccgtggcg
     3361 gatttcttga gcacagcgac cgcctccgcc agggaatatt tttttccttt ttccaaaagc
     3421 ttcttcgatt ccaacaatcg ccggctcaca gaatctcctt tatcggggat agaaaagcta
     3481 ttcgtccact tcgatgccca tgctgcgggc ggtgcccttg accatttcca cggcggcgtt
     3541 cagcgaattg gcgttcaggt cgggcatttt ggtcttggcg acctcttcgg cctgggaccg
     3601 ggtgatcttc cccaccttgg tcttgttcgg ctcggaactc gccttcgcca gcccggcggc
     3661 cttcttgatc agcgccgaca ccggcggcat cttggtgatg aacgtgaacg accggtcgtc
     3721 gtagaccgtg atgacggccg ggatgaccat tccggcctcc atggacttgg tcctgtcatt
     3781 gaactgcttg cagaaatcca tgatattgac gccgtgctgt ccgagggccg gtccgacggg
     3841 cggcgccggg ttcgccccgc cggcggggat ttgtatcttg atctgtgttt ttactttttt
     3901 agccatgggt tatccttctg ccgaaacgct ctaaagtttt tccacttgca aatagtccag
     3961 ttccacgggc gtggcgcggc cgaagatgga gacggtcacg cgcaatttgg cccggtcctc
     4021 gttgacgtcc tccacgctgc cgatgaagtg gcggaacggc ccgtcgttga tgcgaacgtt
     4081 ctcccccttc tcgaaaagaa cggcgggacg cggcttggtg gtggacggcg cgctgaccag
     4141 ttccaacagg tgctgcacct cggaatccgg taggggcacc ggcttcaccc cgcccaggaa
     4201 ccccgtgacc ccggcggtgt tccgcaccag ccaataggtc tcgttgttga tctccatatc
     4261 cgccaggatg tatcccggga aaaattttct tttcttcacc tgcttcttgt tgcgcttcag
     4321 ctccaccacg tcttccgtcg ggataaggac ctggttgatg agtccgctga gactctgcgt
     4381 ggccacggcc ttctccaggg aggatttcac cttgtcctcg tggccggaat acgtatgaat
     4441 cacataccaa gctcgcccca tcctcgaacc cctttcgcta gatcaacaat ccgaacattc
     4501 gggcgatgat gtagtccacc acgcccacgt aaatggagaa aatgatgacc aaaaggatca
     4561 ccagccacgt ggaagccagc atctgggtgc ggctcagcca cgagactcgc ttcaactcgt
     4621 cgtacgcttc acgaaaaaac cgcagaatgt ccgtcatacg aaaccgtccg ttgccccctg
     4681 ttccgaaaaa tcccccatcc cgggggagcc gataacgtga cgatggtttg ggttcatcaa
     4741 cgggttatcg cggaaaggcg taaaattata actcatcctg ccgaaaccgt caacgcgctt
     4801 tatgcacaac cggaacggga aaccgccgac cgttcgccca ccaaactggc aggagcggag
     4861 ggacttgaac ccccagtact ggttttggag accagtggtt taccagttaa ccgacgctcc
     4921 cgtaacaacc attgcgactt gagctgcccc aacgtttcgg ggatccgtgc tttaccggaa
     4981 ccccggatgg gacgctccca aactttcatt gaggagaaga gccgccccaa cgtttaaaaa
     5041 tgcagggacc gcccgatttt ttttgagacg ttcccacaaa tcaaccttcg gcccgcagcc
     5101 gggcatcggc caacggtccg ccgacacccg ccccgtccgc gatccgtcgt ttttatttcg
     5161 tctctttatg ctttgtgtgt ttcccgcagg aggagcagaa ctttttcatt tccagtttct
     5221 tctcccgacg gcggccccgc acatagtgat agttgcggtt gtcgcattcg ctgcaggcca
     5281 acatgtaaac aaaacgctct tttgccatat ccgtagtccg aaggttacgc ggaggaggcg
     5341 gcggtcaacc gcccgcccgt ctctcttttt ccccgctccc cgtcggctcc aacctcccgc
     5401 ctgtttttat tggacgatct cggtgacaac gccggcgccc accgtgtggc cgccttc
//
LOCUS       DAOTFD010000181         5422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10876_length_5422_cov_3.888578, whole genome shotgun sequence.
ACCESSION   DAOTFD010000181 DAOTFD010000000
VERSION     DAOTFD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10876_length_5422_cov_3.888578"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5350)
                     /locus_tag="P5079_09260"
     CDS             complement(<1..5350)
                     /locus_tag="P5079_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30207.1"
                     /translation="MRGVGLKLQKLAYLETLGVPVKEIQKDILAIPFPELVETVEELL
                     REKQPQSITQFSIRSRWEALRERKQQLSPADRVAAVVARLMVENINAGTDVGEIPQEL
                     FNELARRPSFLRILAYGLTPESIRRIVTSAPADLSGTEKAIRTALGEPLADGANWVDV
                     VRDTDVLMQEQENAGWDYLPAQINRIRDNARQAGRLQEPAAESLGKLLYQRALLRLNI
                     TYFQIKYGLYGATYGARDTDKKIRETLLNLATFSWEPGDGYHEFGGFRMGGFTKYPID
                     YGLNADDVLLDLTATQHGAFHQITSLEDLSGFYAELDSGLYAATPAYTPIPVRTSDIL
                     GAVPQANTGWAEDGSLLAASHNDGVTLQFSSVMQNIPVRQMLEEVEADLPDNVLELRD
                     AVSGLLGRLMGDLAPSSGNTQEDLYRTLEGIYLCLKEERARTANAARKRLADLKSKTR
                     AKADKLQAQIDKLEPTNPKRAALQAKLALLQEESLAQKKELEEKIAQLTFKAFKKLYT
                     TPTQDELRQGRDIPAPFKIGAVMDYNRVLERLAQNIQSFRKGKWEVQLDTYGRGPRRT
                     GFDYYYTGGDPVKMQFLAHVLYRLPAALLPKDAAWSGGLDPATAVNLLRSLPKNPQEL
                     MQRLPLSDAQKQQIGAFYGKLASAEAGDWLDRLEQPTQISDVEDADLRGLLEQLKREE
                     LKLLMERMRQGDRNVWAARQVPAAEKEAKADSLEWAGQYDAFQTEAPQKPAPVPQFVL
                     SESDEQAVQELLALIAQGPAGREEAAKRAQSRFGISKSALQEALLGFFPDEDDAQVYR
                     WPVPKPQGYALPASSGNIGKSVGDFFTEHITSNVLRELMAKHLQQRVSKTKHWLYKRM
                     PKAWQEVLQYDRLNGLKALFTQMAMWGKRFGAEDNLVMVAAMGDYFVSQQGRTLDLVI
                     GMKKPPEDERANEVWKQVMEKGWKTALLQRVRGLENVFLAQLDQLVDASLLSSQPLTV
                     ADLEGMAKTEDFGFLAGVSLADVADILALRLNVSIVPMDWLLEKGDYAGQSTDANTRG
                     KLLTRMVTEGILLSGPKFNEPAVMDLVPAENLLNRAAELVASAEAEARTAPEVARGRL
                     SLAHAILEQATRFVAMPADQREESVEERPPLEGPNGYIRYLQDRLAAAHTLLLRDTVR
                     GFFHKQSKALPEETEAPGWLGGVGDGSLEVLYEMAGIEKGRRPSAVTREEGMRIAQAL
                     EARVVTGESADRFAKYVAGVTSTFGKADGSIRPVSMKNNVLTTQSLSEETLTIDVRLV
                     DAPELLAEKHHRLAVLLPRDRTPEEERAGKKGRFYTVYFQSALLDMDPTIQGLVAKSI
                     VEEIKGISQGKSLFAASEEVQSRYKNQEEFLRQEVRRRAQGIVKVRFSEPDKIVVLPE
                     DAPSTGTAAEAGAETATDTGDVVEPELADEEEAEEEETGEEDLLEEPEKKDISPETPP
                     SLAPVTPEQVPQRLAQAAADYIKALDLPRVALVNDDGTEASKWMARVLAANGITVHVT
                     KNVSLPAFSGGLKTAGTYALGLYGDTLLGPQGDIFAGEKMATLRSRAAGLNSAYAMEW
                     DNAVSQDRIQDAPEDWDSRYLAGLETDPVLDLSVIRSGRVQMVVDLRKMSPASQKALR
                     QLFDRIGVHGQYLLEGGSDETLPNLLKKGKGAARLGVVFEEHAQRMTLVDADGQTLSS
                     DDVALLFARHLNGKISGRRIAKSPMDTKLLETLAERFGVELDEVSRPAGAEGNFVMDD
                     WRPWNDVLAQALLAAELAGTAGKPLSTVLQQLYEEMGEPNTRERAMDLPPEHRRRID"
BASE COUNT          790 a         1707 c         1777 g         1148 t
ORIGIN      
        1 tgtcgatgcg gcggcgatgt tcgggcggca ggtccatcgc tcgttcgcgg gtgttgggtt
       61 cccccatttc ctcatagagc tgttgcagca cggtggacag cggtttgccg gcggtccccg
      121 ccagttcggc cgccaggagc gcctgggcca gcacgtcgtt ccagggccgc cagtcgtcca
      181 tgacgaagtt cccctccgcg ccggccggcc gggagacctc gtccaattcc acgccgaaac
      241 gttcggcgag ggtttccagg agtttcgtgt ccatggggga tttggcgatt cggcgtccgg
      301 aaatcttgcc gttcaagtgc cgcgcgaaaa gcaacgccac atcgtccgaa ctcaacgttt
      361 ggccgtccgc atccaccagc gtcatccgtt gcgcgtgttc ttcgaacacc acccccaacc
      421 gggcggcccc cttccctttc ttcaagaggt tcgggagggt ctcgtccgag ccgccttcca
      481 ggaggtattg gccatgcacc ccgatgcggt cgaagagctg tcggagggct ttctgcgacg
      541 ccggcgacat tttccgaagg tccaccacca tctgcacccg gccggagcgg atgacggaca
      601 ggtcgagcac cgggtcggtt tcaagacccg ccaggtagcg ggaatcccaa tcttccggcg
      661 cgtcctgtat ccggtcctgg ctgacggcgt tgtcccattc catggcgtag gccgagttca
      721 gcccggcggc gcggctgcgc agagtggcca tcttttcgcc ggcgaagatg tcgccctgcg
      781 ggccgaggag ggtgtccccg taaagtccca aagcgtaggt gcccgcggtc ttcagaccgc
      841 cggaaaaagc cgggagagag acattctttg tgacgtggac ggtgatgccg ttggccgcca
      901 acacgcgggc catccatttg gaggcctcgg tgccgtcgtc gttcaccagg gccactcgcg
      961 gcaggtccag ggccttgatg taatccgcgg cggcctgggc cagccgctgc gggacttgtt
     1021 ccggcgtgac cggcgccagg gacggcgggg tctccgggga gatgtctttc ttttccggtt
     1081 cttccaggag gtcttcttcg cccgtctctt cctcctcggc ctcttcctcg tcggcgagtt
     1141 ccggttcgac gacgtctccg gtgtcggtgg ccgtttcggc tcccgcttcg gcggcggtcc
     1201 ccgtcgacgg ggcgtcttcg gggagcacca cgattttgtc gggctccgag aaacgaacct
     1261 tgacgatccc ctgcgcccgc cggcggactt cttggcggag gaattcctct tggttcttgt
     1321 agcggctttg gacctcttcg ctggcggcga agagggattt cccctgggag atgcctttga
     1381 tctcctccac gatggatttg gcgacgagtc cctggatggt ggggtccatg tccaacaggg
     1441 cgctctggaa atacacggtg tagaaacggc ccttcttgcc ggcgcgttct tcttccggtg
     1501 tgcggtcgcg cggcagcagg accgccagac ggtggtgttt ttcggcgagg agttcgggcg
     1561 cgtccaccag gcgcacgtcg atcgtcaggg tttcctcgga gagggactgg gtggtgagca
     1621 cgttgttctt catgctgacg ggacggatgg agccgtcggc tttcccgaaa gtggacgtca
     1681 cgccggcgac gtatttggcg aaccggtccg cggactcgcc ggtcaccacg cgcgcctcga
     1741 gggcctgggc gatgcgcatc ccttcttctc gcgtgaccgc ggacggccgg cggcctttct
     1801 cgatgccggc catctcgtag aggacttcca ggcttccgtc gcccaccccg ccgagccagc
     1861 cgggcgcttc cgtctcttcc ggcagagctt tggattgttt gtggaagaaa ccgcgcacgg
     1921 tgtcgcggag aagaagtgta tgggcggcgg ccagccggtc ctgcaggtat cggatgtacc
     1981 cgttgggccc ttccaggggc ggacgttctt caacggattc ttcccgttgg tcggccggca
     2041 tggcgacgaa gcgcgtcgcc tgttccaaga tggcgtgcgc caggctcaac cggccccggg
     2101 ccacttccgg cgcggtgcgc gcctcggcct ccgccgaggc caccagttcc gccgcccggt
     2161 tcaaaaggtt ttccgccggc accaggtcca tcaccgccgg ttcgttgaat ttcggcccgg
     2221 acaacaggat gccctcggtg accatgcgcg tgagcagttt cccgcgcgta ttggcgtcgg
     2281 tgctctggcc ggcatagtcc cccttctcca ggagccagtc catgggcacg atggagacgt
     2341 tcaatcggag cgccaggatg tcggccacgt cggcgagcga aaccccggcg aggaacccga
     2401 aatcttcggt cttggccatg ccctccagat cggcgaccgt taaaggttgg gacgagagga
     2461 gggaggcgtc caccaactga tcgagctggg ccaggaacac gttctccagt ccccgcaccc
     2521 gctgcaggag cgccgtcttc catccttttt ccatcacctg tttccagacc tcgttcgccc
     2581 gctcgtcttc cggcggtttt ttcatgccga tgaccaggtc gagcgtccgg ccctgctggc
     2641 tcacgaaata atcgcccatg gccgcgacca tcaccaggtt gtcctcggcg ccgaaacgtt
     2701 ttccccacat ggccatctgg gtgaacaggg ccttcagccc gttcagacgg tcatactgga
     2761 gaacttcctg ccaggctttg ggcatccttt tgtaaagcca atgtttcgtc ttgctcacgc
     2821 gctgttgaag gtgtttggcc atgagttcgc gcaggacgtt ggaggtgatg tgttcggtga
     2881 agaaatcccc cacggacttg ccgatgttcc cggaactggc cgggagggca tacccctgcg
     2941 gtttgggtac cggccagcgg tagacctggg cgtcgtcttc gtcggggaag aagccgagga
     3001 gggcttcttg gagcgcgctc ttggagatgc cgaagcggga ctgggcccgt ttggccgctt
     3061 cttcgcgccc cgccggcccc tgcgcgatga gcgccaggag ttcctgcacg gcctgttcgt
     3121 ccgattcgga gaggacaaat tgcggcaccg gggcgggttt ctgcggggcc tccgtctgga
     3181 aggcgtcgta ctggccggcc cactccagcg aatccgcctt ggcctctttt tcggcggccg
     3241 ggacctgtcg cgccgcccac acgttccggt cgccttggcg catgcgctcc atgaggagtt
     3301 tcagctcttc ccgcttgagt tgttccagaa ggccgcgcag gtccgcgtcc tccacgtcgg
     3361 aaatctgtgt cggttgttcc aggcggtcca gccaatcgcc ggcctcggcc gaggcgagct
     3421 tcccgtagaa agcgccgatc tgctgttttt gcgcatcgga caacggcagg cgctgcatca
     3481 gttcctgcgg gtttttcggg agggaacgca ggaggttgac ggccgtggcc ggatcgagcc
     3541 cgccgctcca cgccgcgtct ttgggcagga gcgcggccgg caggcggtac aggacgtggg
     3601 ccaggaactg catcttcacc ggatcgccgc cggtgtagta gtagtcgaat ccggtccgtc
     3661 gcggcccgcg gccgtacgtg tccagctgga cttcccattt ccctttccgg aaactctgga
     3721 tgttctgcgc caaccgctcc aacacccggt tgtaatccat gaccgccccg atcttgaagg
     3781 gggccggaat gtcccgaccc tggcggagtt cgtcctgggt gggggtggtg tagagttttt
     3841 tgaaggcctt gaaggtcagt tgggcgatct tttcctcaag ttcttttttc tgcgccaggg
     3901 actcctcttg caggagggcg agtttggcct gcagcgccgc ccgtttggga ttggtcggtt
     3961 ccagtttgtc gatctgggcc tgcagtttgt cggccttggc gcgcgttttg gatttcaggt
     4021 ccgcgagtct cttgcgcgcc gcgttggcgg tccgggcgcg ttcctctttg agacagaggt
     4081 agatgccttc cagtgtccga tagaggtctt cctgtgtgtt gccggacgag ggagccaggt
     4141 cgcccatcaa ccggcccagg agcccgctca ccgcgtcgcg caactccagc acgttgtcgg
     4201 gcaggtccgc ctcgacctcc tccagcatct ggcggacggg gatgttttgc atgacggagg
     4261 aaaactggag ggtgaccccg tcgttgtgcg aggcggccag cagagagccg tcctccgccc
     4321 agccggtgtt ggcctgcggc accgcgccga ggatgtcgct tgtccgcacg gggatggggg
     4381 tgtaggccgg cgtggccgcg tagaggcccg agtccaactc cgcatagaat ccggagaggt
     4441 cttccaacga ggtgatctgg tggaacgcgc cgtgctgggt ggcggtgagg tccagcaaaa
     4501 cgtcgtcggc gttcaggccg tagtcgatgg gatatttggt gaaaccgccc atgcggaacc
     4561 cgccgaactc atggtaaccg tcgcccggtt cccaggagaa cgtggccagg ttgaggagcg
     4621 tttcgcggat cttcttgtcg gtgtcgcggg cgccgtaggt ggcgccgtaa aggccgtact
     4681 tgatttggaa ataggtgatg ttgagccgca acagggcgcg ctggtagagg agtttgccca
     4741 gactttccgc cgccggttcc tgcaaccggc cggcctggcg ggcgttgtcc cggatgcggt
     4801 tgatctgcgc cggcagatag tcccatcccg cgttttcctg ctcctgcatc agaacgtcgg
     4861 tgtcgcggac aacgtcgacc cagttggcgc cgtcggccag gggttcgccg agagcggtcc
     4921 gtatcgcttt ctccgtgccg ctcaggtccg ccggcgccga agtgacgatg cggcggatgg
     4981 attccggcgt gaggccgtag gccaggatgc gcaggaagga gggccgtcgg gccagttcgt
     5041 tgaaaagttc ctgcggtatc tccccgacgt ccgtcccggc gttgatgttt tccaccatca
     5101 accgcgccac cacggcggcc acccggtccg ccgggctcaa ctgttgtttc ctctcccgca
     5161 gcgcttccca acggctgcgg atggaaaatt gggtgatgct ctgcggttgt ttttcccgca
     5221 gcagttcctc gacggtttcc accagttcgg ggaagggaat cgccaggatg tccttttgga
     5281 tttctttcac cggcacgccc agggtttcca gataagccag tttctgcagt ttgagtccga
     5341 ccccgcgcac tttttccggg ccctgcgcgg cgaatcccag gctttcccct tccggctccg
     5401 gagtcccttc tccttagtcc cc
//
LOCUS       DAOTFD010000182         5410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10911_length_5410_cov_4.181699, whole genome shotgun sequence.
ACCESSION   DAOTFD010000182 DAOTFD010000000
VERSION     DAOTFD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10911_length_5410_cov_4.181699"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /gene="ruvX"
                     /locus_tag="P5079_09265"
     CDS             complement(<1..359)
                     /gene="ruvX"
                     /locus_tag="P5079_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRY30208.1"
                     /translation="MIRLGLDWGRARIGVAVSDELGLMAHPVARVPFLSAEQVVEEII
                     RLCRERGASEIVVGLPLNMDGSEGESARSVRDWAAKIGSAAALPVVLWDERLTSWEAD
                     RRLREKGTSPLKKKEKR"
     gene            complement(438..1274)
                     /locus_tag="P5079_09270"
     CDS             complement(438..1274)
                     /locus_tag="P5079_09270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY30209.1"
                     /translation="MTQDIAACSEKGQPEGSAWVLRRYHAVSLILCGVMLGSVALFSR
                     DSEFFPTQPRYPYRIALTFDDGPHPAFTDRLLRVLSENKAVATFFVVGTQAEKYPVLL
                     RTLARQGHELANHTFHHRNLTTISEETLLQELGDTARLIEETTGKRCRYFRPPGGRYN
                     QGTLDLASHNGYRMILWTVFPRDHSAVSAQEVYDRVMASASDGGVVLLHSGMEGTLRI
                     LPKIIQDLRARGFEFLTISEMLHERISPRVLSSWYLPKTPPPALEDDTYAVAGTDLSV
                     DL"
     gene            complement(1285..1851)
                     /gene="thpR"
                     /locus_tag="P5079_09275"
     CDS             complement(1285..1851)
                     /gene="thpR"
                     /locus_tag="P5079_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRY30210.1"
                     /translation="MRLFVAIPPVSGSLGWVREVKDLLRPRLRGSRWVDEENVHVTLK
                     FLGEVPVERLDQVESVLSGVARSFSEFPLELKGLGAFPSVKRPQIVWAGVGEGSSSVV
                     SLAAAVEEALETAGFVREERTFHPHATLARIKEPVVRRESLWPPEWNTRRFSVFTVRN
                     FCLMESRLRPEGPVYQKGKNYLLGPRRG"
     gene            1991..4189
                     /locus_tag="P5079_09280"
     CDS             1991..4189
                     /locus_tag="P5079_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY30211.1"
                     /translation="MKSRFRLRLFLKVALLMVGLATVPLSIVGVQTLLSNREILQYEV
                     LRYHQSLAQSLSNKLDAQLANMEEKLRFIVNFLQSPDSSWQEKQTLLQSVVDASPQLA
                     IIAVVAQDGNEFIKVYSPSREPTLAAFPTLSSHADIPLFKKFMTSKERVFEVSGETED
                     PRFKAYYPFETSTGRHAIFISNSLDNFWKEITNTHIGVSGFAFLADKDGLVLAHPDSA
                     KTAQKTSVRSMPLVSSALAGNIGASEFADTAGKWVGAGAPVRRLGGAVVTLQPRDEAY
                     AAAQKSQRTAVLWLIVSAIIAAILAYVFARRLTRPILSLIKSARTFNLETGRFPDPVI
                     VKTNDEIRDLAETFNSMTQELKNYAALQVERMLAEKTKTEAIVFSISDGLIMTDHQGF
                     IEFINHRARQILDITTDYQSLLKKPLWDFLPHTEVLDVLWDITHNPEQKNLEMDLTST
                     GLRRVFAVSSEQVRTPKGEDIGIVIILHDITLEKEIEQMKDDFLHSITHDLRNPMASI
                     RGFLKFLMDGLGGPVTEQQKKMLETMDRASMRLLGMINDILDVAKLESGKMDLNLSET
                     DLQETSKHVLELLQSQASKKNIQLLIDSPPDLPTVQADPLLMERLLTNLVGNALKFTP
                     EKGKVTIELRDEPNRLCGAVVDTGEGIPPEFVDKIFDKFQQVTGQRKGGTGLGLTICR
                     YIVESHKGKIAATSEVGKGSRFHFWLSKGLMKTPSGEVVIGAAAAAEKPA"
     gene            4186..>5410
                     /locus_tag="P5079_09285"
     CDS             4186..>5410
                     /locus_tag="P5079_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY30212.1"
                     /translation="MISHGPMPNSRKKLLFIVLLSFSLLASVLIPPVPLQGAEALQEI
                     IVKPGDTLWGIANYYLKDPKRWPELLKYNKQLTNNPTVALPGMRLLVPVVLIKEQLRA
                     AELIYLLKDVRTRRKSSNEWKTAKVGMELHNEDGLRTMSESIARIKFPSGEVVSLNEN
                     SLVLIRPEKGEEYVQLMAGDVRARQSRVITAGGAQVTPLGPDSDYRTRVKLDKSELVL
                     VYRGRVDVTAQGKTVRVPEGFGSEIKPLMPPADPVPLPQIPLVQSADMPMPKNMDITV
                     KTQQTERDLVLAVEIPQTKDVGPKSPTTSAEARLQAKAVSRSHTIARYHIQVDDADNF
                     QNPIVDKYAHLSQKFNLLQLGLPNGTYWWRISFIDSLGMEGSFSEPKTFEVDLSPPNM
                     VITYPAEFQEFPWDEE"
BASE COUNT         1196 a         1757 c         1533 g          924 t
ORIGIN      
        1 tcgcgttttt ccttcttctt caacggggaa gtcccctttt ccctgagccg gcggtccgcc
       61 tcccaggagg tgaggcgctc gtcccacagg accaccggga gggccgccgc cgacccgatc
      121 tttgccgccc agtcccggac cgaccgggcg ctttccccct cgctgccgtc catgttgagg
      181 ggcaagccga ccacgatctc cgaagccccc cgctcccggc aaagccgaat gatttcctcc
      241 acgacctgtt ccgcggaaag gaagggcacc ctggcgacgg gatgcgccat gaggccgagc
      301 tcatcggaga cggccacccc gatccgggcc cgaccccaat caagtccaag gcggatcaaa
      361 acgaaaactc ctcgtcacgg aacaacggtc gtgaatatcc gaaactcagg aagggccggc
      421 gccgactcac cggagaacta taaatcgacg gaaaggtccg tgccggcgac ggcatacgtg
      481 tcgtcctcca gggccggcgg gggcgtcttg gggagatacc aggaagacag aacgcgggga
      541 ctgatcctct cgtggagcat ttctgaaatc gtcaggaact cgaaccctcg ggcgcggaga
      601 tcctggatga ttttcggcaa tatcctcaac gtcccctcca taccggaatg aaggaggacc
      661 acgccgccgt cggaggcgct ggccatgacc cgatcgtaga cttcctgcgc cgacaccgcc
      721 gagtgatcgc gggggaacac cgtccaaagg atcatgcgat atccgttatg ggaggccagg
      781 tccagggtcc cctggttgta ccgtccgccc ggtggacgga aatagcggca tcttttcccg
      841 gtggtttctt cgatgaggcg ggcggtgtcg cccaattcct gaagcagcgt ctcctcggaa
      901 atcgtggtca ggttcctgtg gtgaaaggta tggttggcca actcatgtcc ctggcgggcg
      961 agcgtgcgga gcagcacggg atatttctcc gcctgagtcc cgacgacgaa aaaggtggcc
     1021 acggccttgt tctcggagag cacgcggagg aggcggtcgg tgaaagccgg atgggggccg
     1081 tcgtcgaagg tgagggcgat gcggtaagga tagcggggct gggtggggaa aaattcgctg
     1141 tcccgggaaa acagggcgac gctgccgagc atgacgccgc aaaggatgag ggacacggcg
     1201 tgatagcgcc gcaaaaccca ggccgaacct tccggttgtc ccttttccga gcacgccgcg
     1261 atgtcttgtg tcatcccgtc cccctcaacc cctccgcgga cccagaaggt aattcttccc
     1321 cttttggtaa accggccctt ccggccgcaa acggctctcc atcagacaaa aattccgaac
     1381 cgtgaaaacg gaaaaacgcc gcgtgttcca ctccggcggc cagaggctct cgcgccgcac
     1441 gaccggttct ttgatgcggg cgagagtcgc atggggatga aacgtccgct cttcccgcac
     1501 aaaaccggcc gtttcgagcg cctcttccac cgccgccgcc aaggagacga cggaggaact
     1561 cccttcgccg acgcccgccc acacgatctg cggcctcttt accgacggga aagcgccgag
     1621 gcctttcaat tccaacggga actccgaaaa agaccgcgcc accccggaca gaaccgattc
     1681 cacttgatcc aggcgctcca cgggcacttc tcccagaaat ttcaaggtga cgtggacgtt
     1741 ttcctcgtcc acccaacggc tcccccggag gcggggccgg aggaggtcct tgacttcccg
     1801 aacccacccc agggaacccg aaaccggcgg gatggccaca aaaagcctca tccgcgccgt
     1861 ctccggacaa gccgacgttc cgcgcgcacg tttctaaaat agcatagacc cccggaaaac
     1921 gcaatgcgga cggctcgaat cctcaaaagg cattgcgccg agagggaagg tatattaaac
     1981 ttaaaatcta atgaagtcgc gattccggct ccgtcttttc ctaaaagtcg cgctgctcat
     2041 ggtgggtctg gccacggtgc cgctctccat cgtgggcgtt caaacgctcc tcagcaaccg
     2101 tgagatcctg cagtacgagg tcttgcggta ccaccaaagt ctcgcccaat ccctctccaa
     2161 caagctcgac gcgcaactgg cgaacatgga agaaaagctc cgtttcatcg tcaatttcct
     2221 ccaatccccc gattcgagct ggcaggaaaa gcagaccctc cttcagtccg tggtggacgc
     2281 cagcccgcag ctggccatca tcgccgtggt ggcgcaggac gggaacgaat tcatcaaggt
     2341 ctacagccct tcgcgggagc cgaccctggc ggcgttcccc acgctcagct cccacgccga
     2401 cattcccctc ttcaagaaat tcatgacgtc gaaggagcgc gttttcgagg tctccggaga
     2461 aaccgaagac ccccgcttca aggcctacta tccctttgaa accagcacgg gccggcatgc
     2521 catcttcatc agcaactccc tcgacaattt ctggaaggag atcaccaaca cgcacatcgg
     2581 cgtctccggg ttcgcgttcc tggcggacaa agacgggttg gtcctggccc atccggacag
     2641 cgccaaaacc gcccaaaaga cctccgtccg gtccatgccc ctggtgtcct cggccctggc
     2701 gggcaacatc ggggcctccg aattcgccga caccgccggc aaatgggtgg gggccggcgc
     2761 gccggtgcgc cgtctgggcg gcgcggtggt gacgctgcag ccccgggacg aggcgtatgc
     2821 ggcggcccag aaaagccagc gcacggccgt cctctggctc attgtttccg ccatcatcgc
     2881 cgccatcctg gcctatgtgt tcgcacggcg gctgacgcgg cccatcctct ctctgatcaa
     2941 atccgcccgg acgttcaatt tggagacggg ccgattcccc gatcccgtca tcgtcaaaac
     3001 gaacgacgag attcgcgacc tggccgaaac cttcaattcc atgacacagg aactgaaaaa
     3061 ctacgccgcc ctccaagtgg aacgcatgct ggcggaaaaa acaaagaccg aggccatcgt
     3121 cttctccatc agcgacggtc tcatcatgac ggaccatcag gggttcatcg agttcatcaa
     3181 ccaccgggcc cgccagatac tggacatcac gacggactac cagtcccttt tgaaaaaacc
     3241 gctctgggac ttcctgcccc acacggaagt gctggacgtt ctctgggaca tcacccacaa
     3301 tccggaacag aaaaatctgg agatggacct gacctcgacg ggtctccggc gggtgttcgc
     3361 ggtgtcctcg gaacaggtgc gcacccccaa gggagaagac atcgggatcg tcatcatcct
     3421 gcacgacatc acgctggaaa aagaaataga acagatgaag gacgacttcc tccattccat
     3481 cacccacgac cttcgcaacc ccatggcctc catccggggc ttcctcaagt tcctcatgga
     3541 cgggctcggc ggccccgtca ccgagcaaca gaaaaaaatg ctggaaacga tggaccgggc
     3601 cagcatgcga ttgctcggca tgatcaacga catcctggac gtggccaaac tggaatcggg
     3661 gaagatggat ctcaacctga gcgagaccga cctccaggag acctccaaac acgtgctgga
     3721 gttactgcag tcgcaagcct ccaagaagaa catccagctc ctgatcgatt cgccgccgga
     3781 cctgcccaca gtgcaggccg accctctcct catggagcgg ctgctgacga acctggtggg
     3841 caacgccctc aagttcaccc ctgaaaaagg gaaggtcacc atcgaactgc gcgacgaacc
     3901 caaccggctc tgtggagcgg tggtcgacac cggggaaggg atcccgccgg agttcgtcga
     3961 caaaatattc gacaagttcc agcaggtcac cggccaacgg aagggcggca cgggcctggg
     4021 gctgacgatc tgccgctaca tcgtggaaag ccacaaggga aagatcgccg cgacgtccga
     4081 agtggggaag ggatcccgtt ttcacttctg gctcagcaaa gggctgatga agaccccctc
     4141 cggagaagtc gtgatcgggg cggcggccgc ggcggagaaa ccggcatgat ctcgcacgga
     4201 ccgatgccga actcccgcaa aaaactcctc ttcatcgttc tcctgtcgtt ttctctcctc
     4261 gcttccgtcc ttattcctcc cgtccccctt cagggcgccg aggcgctcca ggaaatcatc
     4321 gtaaaaccgg gagacaccct ctggggcatc gccaactatt acctgaagga ccccaaacgc
     4381 tggccggaac ttctgaaata caacaagcag ttgaccaaca accccacggt cgccctgccg
     4441 gggatgcggc ttctggtacc ggtcgtcctc atcaaggaac aactgcgcgc ggccgaactg
     4501 atctatctgc tgaaggatgt ccggacccgt cgtaaaagca gcaacgaatg gaaaacggcg
     4561 aaggtgggga tggaactgca caacgaggac ggcctccgca ccatgagcga gtccatcgca
     4621 cggataaaat ttcccagcgg agaagtcgtc agcctgaacg agaattccct cgtgctgatc
     4681 cggccggaga agggcgagga atacgtgcaa ctgatggccg gcgatgtacg ggcgcggcag
     4741 tcgcgggtga tcaccgcggg gggagcgcag gtcacgccgc tcggcccgga ttccgactac
     4801 cggacgcgcg tcaagctgga caaatccgaa ctggttctgg tctaccgggg ccgggtggac
     4861 gtcaccgccc aagggaaaac cgtccgggtg cccgaagggt tcggctcgga aatcaaaccc
     4921 ctcatgccgc cggcggaccc cgtcccgctt ccgcaaatcc ccctcgtcca gtccgccgac
     4981 atgcccatgc cgaaaaacat ggacatcacg gtgaagacgc aacagacgga gagggacctg
     5041 gtgctggcgg tggaaatacc gcaaaccaag gacgtcggcc ccaaatcccc cacgacctcg
     5101 gccgaggccc gcctacaagc caaggcggtc tcccgaagcc acacgatcgc acgttaccac
     5161 attcaggtgg acgatgcgga caatttccag aatccgatcg tcgacaaata cgcccatctc
     5221 tcccagaaat tcaatctcct ccaattgggc ctcccgaacg ggacatactg gtggcgcatc
     5281 tctttcatcg attctctcgg gatggaaggt tccttttccg aaccgaaaac ttttgaggtg
     5341 gacctctcgc cacccaacat ggtgatcacc tatccggcgg aatttcagga atttccctgg
     5401 gacgaggaat
//
LOCUS       DAOTFD010000183         5408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_10919_length_5408_cov_3.714179, whole genome shotgun sequence.
ACCESSION   DAOTFD010000183 DAOTFD010000000
VERSION     DAOTFD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10919_length_5408_cov_3.714179"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /gene="argS"
                     /locus_tag="P5079_09290"
     CDS             <1..1367
                     /gene="argS"
                     /locus_tag="P5079_09290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRY30213.1"
                     /translation="RGEYARRPVSAEKPLLIEFVSANPNGPLHVGHGRGAALGDSLAR
                     IFRHLGYAVSTEYYVNDVGNQMRNLGVSVMWRILELRPDYLTAEERATVEGWNKEELY
                     KGDYVYDIALAVTGRYPKTVSRPEGIDFFRQRGIEHTLEMIRTELERFGVSMDEWFSE
                     GRLYEQNLVDKSLDTLRRRGFLKEEAGALWFLSTKFGDDKDRVIKRKDERPTYFASDI
                     AYHAHKFERGFGRLIDIWGTDHHGYVRRVKAAVEAMGNNPDDLTILLYQLVSLVRGGK
                     PVAMSTRAGEFVTLREVLDEVGKDACRFFFAMRSPDSQLEFDLDLAKKQAPENPVFYV
                     QYVHARCCSIFRESEKRGFFVEDTSRFAPPEELAAEERGLLVRLVSYPDVVEQCRRDM
                     SPHHLTTYLLALAGDFHRFYENCRVLDESRKVMLFRLALTDAVRVIIRSGLSLLGVGA
                     PETM"
     gene            1369..2097
                     /locus_tag="P5079_09295"
     CDS             1369..2097
                     /locus_tag="P5079_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30214.1"
                     /translation="MHKILIVDDDPAIRELVGRMLEKEGFSVQSVESAEDALRFLRMT
                     LFDLIVLDLNLPGISGMKMCEILKQDPATATLPVIMLTSASAERSKVQGLQTGADDYI
                     VKPPSPPELMARINALLRRVKYGGVPAKVLEAGGPEWTWTAMRRRSMGNRSSCVPRSS
                     SCWRSFWKRKAACCLGRPFSTPCGRTRLFPNIPWRSTSTICVKNLGPRRLACRPFPAS
                     AINSKAFELARAHLPPRRIVQTYP"
     gene            2238..2648
                     /locus_tag="P5079_09300"
     CDS             2238..2648
                     /locus_tag="P5079_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY30215.1"
                     /translation="MKKILVIDDDADMRLYVSSILQAQGFEVKSVETGCEAMSAMRNE
                     FFDLVVMDILLPDTNGLDLCKKVKEFNVSSMPVILLSSVTDDAVIRSGLEEKKADAFM
                     AKPPSPLELVRRALGLLNGAGSQGGALGRASTAI"
     gene            2840..4492
                     /locus_tag="P5079_09305"
     CDS             2840..4492
                     /locus_tag="P5079_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30216.1"
                     /translation="MILFYAALALLSMGRVLPHFQESAFTISPYDFGNNLWRFWWGPF
                     SFYNGLNPYFTQWQFHPFGAPLTVNFSLMDYFFTVPLSGLLNPVGLYNLTLTLTYVFT
                     GVLSFHLFYFFSKHDRGALLASVFVTFSPFKNYHVLVSGSEVAQTHWPILLLLAYFLI
                     YFNDTWGWKALWALPAVFPAIYHEQRTLMMAVLFLTVVTFVDIVEKAVRRDPVWKKRL
                     AKYLVFCAGAAVLSLPLLWEYLRVFGDWNGASSPGIHVGGWKQIFLWPAHYFLRFESG
                     NGELLFERNAVYLNVFAMGLAVLGALKARALAGRFGFIVGFFVFLGFALGYWSLSGLP
                     MVGYLKAPARYAFIVSLVVGLFLACGWRAFETMFRPKALRGVLYGALMVLFFVETNPL
                     FCGPFDIKKSAALEELRKNTERGAVLDLPFGIAPRVVGRFYYKSLLRATQHEKPVMNG
                     FIAWLDIATRKKAKAGPFHRAIAGCEFEGRCEAVGDEFWRKLATGWGVKYVICDNGFE
                     NDPLIARVRTSPVFERVASEAEFDLYVFKAAGKTRKKGSEGE"
     gene            4489..5268
                     /locus_tag="P5079_09310"
     CDS             4489..5268
                     /locus_tag="P5079_09310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30217.1"
                     /translation="MKKIVVFLPTLNECANLQWLLPRFEQASLPQFTLLVVDDDSRDG
                     TRDFLNSYKSHSFDLRLIVRRGDPGRGLAGQVAFAFAHKTGADVLVEMDADGSHDPCD
                     VASLMDGLGPGVGMVLGCRKNFLDKKGEQCHVFRKMMTIVSNAFVRRLFNVPYRDGNS
                     GFRCYSRDAIDRLVRISLKARGCEIVHETLFKARRSGIRIAERPIEFYARRSGVTTKK
                     FHDFVRVFAVCLLLKAGLWDRFCVFGRSVGGGALEENPSSV"
BASE COUNT         1000 a         1451 c         1696 g         1261 t
ORIGIN      
        1 aacgggggga atacgcccgc cggccggtgt ccgccgaaaa acccctcctc atcgaattcg
       61 tttccgccaa ccccaacggc cccctgcacg tggggcacgg ccgcggcgcc gccctgggcg
      121 acagtctggc ccggatcttc cgccatctcg ggtacgccgt ttccaccgag tattacgtga
      181 acgacgtggg aaaccagatg cggaacctcg gcgtgtccgt gatgtggcgg atcctggaac
      241 tgcggccaga ctacctgacg gccgaggaaa gggcgaccgt ggagggatgg aacaaggagg
      301 aactttacaa gggcgactac gtgtacgaca tcgccctcgc cgtgacgggg cgttacccga
      361 agacggtttc ccggcccgaa ggcatcgatt ttttccggca gagaggcatc gaacatacgt
      421 tggagatgat ccgcacggag ctggagcggt tcggcgtgag catggacgaa tggttttccg
      481 agggccggct ctacgaacag aacctggtgg acaagtcgtt ggacacgctg cgccggcggg
      541 gtttcctcaa ggaggaggcc ggcgcgctgt ggttcctttc cacgaagttc ggcgatgaca
      601 aggaccgggt catcaaacgg aaagacgaac ggcccaccta tttcgcctcc gacatcgcct
      661 accatgccca taagttcgag cggggcttcg gccgcttgat cgacatttgg ggcaccgacc
      721 accacggata cgtgcgccgg gtgaaggcgg cggtggaggc gatggggaac aaccccgacg
      781 atctgacgat cctcctttat caactggtga gcctggtgcg cggcgggaaa cccgtggcca
      841 tgtccacgcg ggcgggggag ttcgtcaccc tgcgcgaggt gctggacgag gtggggaaag
      901 acgcttgccg gttcttcttc gccatgcgca gcccggacag ccagttggag ttcgacctgg
      961 acctggccaa aaaacaggcg ccggagaacc cggtgttcta cgtgcaatac gtgcacgcac
     1021 ggtgttgttc catcttccgg gaatcggaaa agcggggttt ctttgtggag gacacctccc
     1081 ggttcgcccc tccggaggaa ttggccgcgg aggagagagg tctgctggtg cgcctggtct
     1141 cctatccgga cgtggtggag cagtgtcggc gggacatgtc gccgcatcac ctgaccacct
     1201 acctgctggc gttggctggg gatttccacc gtttttatga gaactgccgc gtgttggacg
     1261 agtcccgtaa ggtgatgctg ttccgtctgg cgttgaccga cgcggtgcgg gtcatcatcc
     1321 gcagcgggct ttcgcttttg ggcgtgggcg ctccggagac gatgtgatat gcacaagatc
     1381 ctgatcgtcg acgacgaccc ggccatccgt gaactggtgg gtcggatgct ggagaaagaa
     1441 gggttctcgg tccaatccgt ggaaagcgcc gaggacgccc tgcggttctt gaggatgacg
     1501 ctgttcgatc tgatcgttct ggacttgaac cttcccggca tctccggcat gaagatgtgc
     1561 gagatactca agcaggaccc cgccaccgcg acgctgcccg tgatcatgct gacctccgcc
     1621 agcgccgaac gatccaaggt gcaggggttg cagaccgggg cggacgacta catcgtgaaa
     1681 ccgccgtcgc cgccggagct gatggcgcgg atcaacgcgt tgttgcgccg tgtgaaatac
     1741 ggcggcgtgc ccgccaaagt gctggaggcg ggggggcccg agtggacatg gaccgccatg
     1801 aggcgtcggt ccatgggaaa ccgatcgtct tgcgtcccaa ggagttccag ttgctggcgc
     1861 tctttctgga aaagaaaggc cgcgtgttgt ctcggtcggc cattttcgac gccctgtggt
     1921 cggacgaggt tgtttccgaa cataccttgg aggtccacat caaccatttg cgtgaaaaac
     1981 ttgggaccgc gtcgtcttgc ctgcagaccc tttccggcgt cggctataaa ttcgaaggcg
     2041 tttgagctcg cccgggccca cctccctccc cgccgcatag tacaaacata tccataaatc
     2101 aatgcgtttt tgaatacgct gaagagggca ccggaggttt gagggtcttt tgagggcgct
     2161 ttgagggaat tttgagcttc ctcgggtatg ctttaagtgg aagtgcggca tcaagagcgg
     2221 cgcgtgggag aattgccatg aagaaaatat tggtcatcga cgacgacgcg gacatgcgtc
     2281 tctatgtgtc ttccatcctg caggcgcagg gcttcgaagt gaagtccgtt gaaacgggct
     2341 gcgaagccat gagcgccatg cggaatgaat tttttgatct ggtggtgatg gacatcctcc
     2401 tgccggacac caacggcctc gacctctgta agaaagtgaa ggagttcaac gtgtcgagca
     2461 tgccggtgat cttgttgtct tccgtcaccg atgatgccgt catccgaagc ggtttggagg
     2521 agaaaaaggc ggacgccttc atggccaagc cgccctcccc tctggaactt gtgcgccgtg
     2581 cgctcggcct gttgaacggg gccggttccc aggggggggc gttggggagg gcctccaccg
     2641 ccatttgaag tttccaacac cacgatcccc gccagaaatg tctaaagaca ttccacccaa
     2701 tcctgaccga gtcgaaatcg ataaaaccaa ccctgttcgt ttgagggaat acatcgaccg
     2761 tgaagggcat gtgaaacggt ggatttcttt cccttctcgg gcgatcttct tgagccccgc
     2821 tgtccgtcgg acgatggggt tgatattgtt ttatgcggcg ctggctcttt tgagcatggg
     2881 tcgcgtcctt ccacatttcc aggagagcgc cttcaccatc agcccttacg acttcgggaa
     2941 caacctttgg cggttctggt gggggccgtt ctctttttac aacggtctga atccttattt
     3001 cacccagtgg cagttccatc cgttcggggc cccgctcacc gtcaattttt cgctgatgga
     3061 ctatttcttc acggtgcctt tgtccggttt gctgaatccc gtcggcctct ataaccttac
     3121 gctcaccctc acttacgttt ttacgggtgt gctcagtttc catctgttct attttttctc
     3181 gaaacacgac cggggggccc ttttggcttc tgtttttgtc actttctctc cgttcaaaaa
     3241 ttatcatgtc ctcgtcagtg gttcggaagt cgcccagacg cattggccga tactccttct
     3301 cttagcgtat ttcctgatct attttaacga cacgtggggt tggaaggcgt tgtgggcgct
     3361 gccggctgtc tttccggcga tataccatga acagcgcacc ttgatgatgg cggtgctgtt
     3421 cctgacggtg gtgacgttcg tggatatcgt ggaaaaggcc gtgcgtcggg acccggtctg
     3481 gaaaaaacgg ctcgccaaat atctcgtttt ctgcgccggc gcggcagttt tgtctttgcc
     3541 tcttctgtgg gaatatttga gggtgttcgg agactggaat ggggcttcaa gtcccggtat
     3601 ccacgtcggc ggatggaaac aaatcttcct atggccggcc cattattttt tgaggtttga
     3661 atcggggaac ggggagctcc tcttcgagag gaacgccgtt tatctgaacg ttttcgccat
     3721 ggggttggcc gttttaggcg cgctgaaggc aagagctctt gccggacgct tcggttttat
     3781 cgtcggtttc ttcgtgttcc ttgggtttgc gctaggatac tggtctctgt ccgggcttcc
     3841 gatggtgggc tatttaaagg ccccggcccg ctacgctttc atcgtcagcc ttgtggtcgg
     3901 gcttttcctg gcttgtggat ggcgggcgtt tgagacgatg tttcggccga aggcgttgcg
     3961 tggggttctg tatggggccc tgatggttct ttttttcgtc gaaacgaacc cgcttttctg
     4021 cgggcctttt gacatcaaaa aaagcgcggc gctggaggag ctgcggaaga atacggaacg
     4081 tggtgcggtg ttggacctcc ccttcgggat cgcgccccgg gtggtcggcc ggttttatta
     4141 caaatctttg ttgcgggcca ctcagcacga aaagccggtg atgaacggtt tcatcgcctg
     4201 gctggacatc gcgacgcgaa aaaaagcgaa ggccggcccg ttccatcgcg cgatcgcggg
     4261 ctgtgagttc gagggacgat gcgaggcggt gggagacgaa ttctggcgga agctcgcgac
     4321 gggctggggc gtcaaatacg tcatctgcga caacgggttt gagaatgatc cgctgatcgc
     4381 cagggtccgg acgtcccccg ttttcgaacg ggtggcgtcg gaggcggagt tcgaccttta
     4441 cgtcttcaag gcggcgggaa agacgcggaa aaaggggtcg gagggggaat gaagaagatc
     4501 gtcgttttcc tgccgacctt gaacgaatgt gcgaacctgc aatggctttt gccgcgattc
     4561 gaacaggctt ctctgccgca gttcacgttg ttggtggttg acgacgacag ccgcgatggg
     4621 acccgcgact tcctcaattc ctacaaaagc cattcctttg accttcgcct catcgtgcgc
     4681 cggggcgacc ctggccgggg attggcggga caggtggcgt ttgcgtttgc gcataagacc
     4741 ggcgcggatg tgctggtaga gatggacgcc gacggttcgc atgacccttg cgacgtcgcg
     4801 tccttgatgg acggcctggg tcccggcgtc gggatggtgt tgggatgccg aaagaacttc
     4861 ctcgacaaaa aaggcgagca gtgccacgtt tttcgaaaaa tgatgaccat cgtttcgaac
     4921 gctttcgtcc gccggctttt caacgtacct tacagggatg gcaacagcgg tttccgctgt
     4981 tattcccgcg acgccattga ccgactggtt cgaatttctt tgaaggcgcg cggttgtgag
     5041 atcgtccatg aaacattgtt caaggcccgg cgatccggga tccggatcgc cgagcggccc
     5101 atcgagtttt atgcgcggcg gtccggtgtc acgactaaaa aattccatga ttttgtgaga
     5161 gttttcgcgg tgtgcctcct tttaaaagcg ggtctttggg accgtttttg cgtgttcggc
     5221 cggtccgttg gagggggggc gttggaggaa aatccgtcct ctgtttgaga tttgcaagtc
     5281 gaccggtccg aaatcgaaaa ttgggcgctt tttgcggggg agatcaaaaa aacccttgaa
     5341 attgggggga tgggcggtca aaccccctgt tcccggtgaa tgcggtccca gctgccctgt
     5401 atctattt
//
LOCUS       DAOTFD010000184         5320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11167_length_5320_cov_3.471225, whole genome shotgun sequence.
ACCESSION   DAOTFD010000184 DAOTFD010000000
VERSION     DAOTFD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11167_length_5320_cov_3.471225"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1062)
                     /gene="pheS"
                     /locus_tag="P5079_09315"
     CDS             complement(1..1062)
                     /gene="pheS"
                     /locus_tag="P5079_09315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY30218.1"
                     /translation="MSSLLNQLHSLRAQAQQDIAQADSLEGLETLRVRYLGRKDGQLT
                     KILQALPSLSPEEKKVLGREANEAKISLETALNEKKGAIEEKTLNAVLSAAAGQDLTL
                     PESPLSVGRRHPLAQVMDDIVGCFLRLGFNLAEGPEVETDFNNFTALNHPPDHPARDS
                     HDTFYVKNFRDGEGLPLLLRTHTSPVQIRHMQTHKPPLSIVAPGRVFRHEAVDATHSF
                     VFHQVEGLAVDVDISVADLKGVLGLFARHVFGSNVEIRFRPAYFPFVEPGIEVDISCT
                     LCEGKGCRVCKQTGWVEMLGAGMVNPNVFRAVGYDPDKVTGYAFGIGVERVAMFKYGV
                     DDMRLFFENDVRFLRQVSR"
     gene            complement(1166..1528)
                     /gene="rplT"
                     /locus_tag="P5079_09320"
     CDS             complement(1166..1528)
                     /gene="rplT"
                     /locus_tag="P5079_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRY30219.1"
                     /translation="MRVKSVVYTRQRKKKWFRRAKGAYATKKNRWRMVIQHLDKSMKA
                     MYRGRKDKKGDFRSVWIQRINAAARLNGLSYSRFMSGLKKAGVTLDRKILADMAMNDQ
                     GAFQQLTELVRTQATTPS"
     gene            complement(1518..1718)
                     /gene="rpmI"
                     /locus_tag="P5079_09325"
     CDS             complement(1518..1718)
                     /gene="rpmI"
                     /locus_tag="P5079_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRY30220.1"
                     /translation="MPKLKTHSGTKKRFRVTASGKVMYQLQGRRHLLIGMSSTRRRRF
                     RKDGMLYAVQAEKIKKLIPYAR"
     gene            complement(1728..2240)
                     /gene="infC"
                     /locus_tag="P5079_09330"
     CDS             complement(1728..2240)
                     /gene="infC"
                     /locus_tag="P5079_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRY30221.1"
                     /translation="MKPAEPRYNHQIRAQQVRLIDQDGTQLGIKSLDEALRVAADRGS
                     DLVEIAPSANPPVCKVLDFSKYRYEREKQRKEAKKHQKGGHVKEIRFRPKISEHDLNT
                     KIRAMQTFLENRDKVRISLVFHGREMEHRELGLRLIDKVKEQLAAVSSVESEPFQLGN
                     RLILMLAPKK"
     gene            complement(2307..4079)
                     /locus_tag="P5079_09335"
     CDS             complement(2307..4079)
                     /locus_tag="P5079_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30222.1"
                     /translation="MRWRLEWDFSLRGNLEAVKKPAPLLPAFLLFLYFVLTLSYFTSP
                     GYIVDGLADEAAGARWADELRNGLPFSLFRFNFHLYNGCLTRYLMMPFFYVFGTSWTI
                     VRLWPIVFGGLTLLVTYRFANAAFKPKAGLLALLFLVIHPAYLMGVKSGNYQVSYMTF
                     FSISSLYFLHRWWLTQKTIPLFLATFLLGLGLGTRFFFIWYGAAFGGVFLLALWDIRT
                     QLLPGVWNKLLKPLFIAGLGFLASTFLMWRTELRIDTPTLPLALQHLAASGHSPSISW
                     DVLHRAIGGFHLFLLGHRPYENFFQASPPGNSLYPAAFWAAFIFCALLPAFRKNLPSR
                     KTVLGLLSLFVVMQIMSVVSHVSRIQGTPRDSQYMFVFFPFPQIVLALAFSQLFALLR
                     ERRFLRGLTIVFLVSFCYGEARLLADYFHRMKETGAGRRFTDAVHDVGEWLRLHPTGR
                     RPVWVMSSDLADNLYCIDPRIKTTVIGSVTEHPLFLCREEDFFPLWEKRLSEQSDREL
                     LIVNDNCGYANYQKDFDLIKSWALQRRKKFGPVAQFRDKDGTVVFEAYLLELGEGEPS
                     RRTSPPGTAGTAPFQSPVDNKKTL"
     gene            complement(4135..>5320)
                     /gene="thrS"
                     /locus_tag="P5079_09340"
     CDS             complement(4135..>5320)
                     /gene="thrS"
                     /locus_tag="P5079_09340"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY30223.1"
                     /translation="LGPRLGLFTIQHEEGGSGFIYWLPKGAIVRRIAEDYLKDFLGAK
                     GYQFVYTPHVARIDLWKTSGHLDYYAENMYPPMKLENQDFQLKPMNCPGHILIYKSEL
                     HSYRELPLRLAEFGTVYRFERSGVLHGLMRVRGFTQDDAHIFCRPDQVEDEVVNIMQT
                     ITDVLKTFGFSDYALKLSTRPDKYSGTLEGWAHAENALKKALERMNLKYAVDPGEGVF
                     YGPKIDLKIKDSLGREWQCSTVQVDFNNPKRFNITYRDEKGGETEVFMIHRALFGSLE
                     RFFGVMIEHYAGAFPLWLAPVQAAVLPISDKFAAYAEEVQNRLKEAGLRVHKDDSASK
                     IGAKIREATLQKIPYLLVVGAREAENKTVSVRTREGKDLGAPPLSDMLKTMLEQVRNK
                     SA"
BASE COUNT         1060 a         1425 c         1781 g         1054 t
ORIGIN      
        1 tcatcgggag acctgcctca ggaaccgcac atcgttctcg aagaacaggc gcatgtcgtc
       61 cacgccgtat ttgaacatgg ccacgcgttc cacgccgatg ccgaaggcat atcccgtgac
      121 tttgtccggg tcgtacccca cggcgcggaa cacgttgggg ttcaccatgc cggcgccgag
      181 catctccacc caccccgtct gcttgcagac gcggcagcct tttccttcgc agagggtgca
      241 ggagatgtcc acctcaatgc cgggttccac gaacggaaag taggccggcc ggaaacggat
      301 ctccacgttg gacccgaaga cgtggcgggc aaagaggccc agcacgcctt tgaggtccgc
      361 cacggagatg tcgacgtcca cggcgaggcc ttccacttga tggaacacga aggagtgggt
      421 ggcgtcgacc gcctcgtggc ggaacacgcg gccgggggcc acgatggaga gcggcggttt
      481 gtgggtctgc atgtggcgga tctgcaccgg cgaggtatgg gtccgcaaaa gcagcggcaa
      541 cccttcgccg tcccggaaat tcttcacgta gaacgtgtcg tgggaatcgc gcgccgggtg
      601 gtcgggcgga tggttcaacg cggtgaaatt gttgaaatcg gtctccacct ccggcccctc
      661 ggcgagattg aagccgagac ggagaaaaca tccgacgatg tcgtccatga cctgcgcgag
      721 gggatgccgt cggccgacgg aaagaggcga ttccggcagg gtcaggtcct gtccggccgc
      781 ggcggacagg acggcgttca gggttttttc ttcgatggcc cccttcttct cgttgagggc
      841 ggtttcaagg gagattttgg cttcgttggc ttcccggccg aggacctttt tttcttccgg
      901 agagagggac gggagggcct ggaggatttt cgtgagttgg ccgtctttgc ggccgagata
      961 gcggacgcgc agggtttcca gcccttccag ggagtcggcc tgggcgatgt cctgttgggc
     1021 ctgggcgcgg agggaatgca gttggttgag gagagaactc atggaaaaac ggtcgttcgg
     1081 ggacgggagc gaatgggaat tcgggcgtgg gacagcggag ccatcgggag cgcggcccgt
     1141 ccgccgcgcc gggcggcgga cgacatcagg aaggggtcgt cgcctgggtg cggacgagtt
     1201 ccgtgagctg ttggaacgcc ccttggtcgt tcatggccat gtcggccagg atcttacggt
     1261 cgagggtcac gccggccttt ttaaggccgg acatgaagcg ggagtagctg aggccgttca
     1321 accgggcggc ggcgttgatg cgctgtatcc agacggaacg gaaatcgccc tttttgtcct
     1381 tgcggccgcg gtacatggcc ttcatggact tgtccaaatg ctgaatgacc atgcgccacc
     1441 ggttcttctt ggtggcgtag gcgcctttgg cccgccggaa ccatttcttc ttgcgttgcc
     1501 gtgtgtagac gacgctctta acgcgcatag gggatcagct ttttgatttt ttcggcctga
     1561 accgcgtaca gcatgccgtc cttacggaag cgacggcgcc gggtggagga catgccgatc
     1621 aacagatgcc ttcggccctg aagctggtac atcaccttgc cggaggcggt gacgcgaaaa
     1681 cgttttttcg tgccgctgtg ggtcttaagt tttggcatag agtttcccta ttttttgggg
     1741 gccagcatca gtatcagccg gttcccgagc tgaaacggtt cggattccac cgacgagacg
     1801 gcggcgagct gttccttcac cttgtcgatc agccggagac cgagttcccg gtgctccatt
     1861 tcgcggccgt ggaagaccag actgatgcgg accttgtccc ggttttccaa gaaagtctgc
     1921 atggcgcgga tcttcgtgtt caggtcgtgt tcgctgatct tcggccggaa ccggatttcc
     1981 ttgacgtgcc cgcccttttg gtgtttcttg gcttctttgc gctgtttctc ccgctcgtag
     2041 cggtacttgg agaaatccaa aaccttgcat acggggggat tggccgacgg ggcgatctcc
     2101 accaggtccg agcctcggtc cgccgctacc cgcaacgcct catccaaaga cttaatccca
     2161 agctgggtgc cgtcctgatc gatcaggcgc acctgttggg cgcggatttg gtggttgtat
     2221 ctcggctcag cgggtttgat gcagattcct cctaaaaaaa acagaggagg tcaatcctcc
     2281 tccgagggtg aagcgagaga atcctatcac aacgtttttt tgttgtcaac aggggattgg
     2341 aagggggctg ttccggcggt tccgggaggg gacgttcgcc gggagggttc cccctccccc
     2401 agctccaaca ggtaggcttc gaacaccacg gtgccgtcct tgtctcggaa ttgggcgacc
     2461 gggccgaatt tcttccgacg ttgaagagcc caggatttga tgaggtcgaa gtctttctga
     2521 tagttggcgt atccgcagtt gtcgttgacg atgaggagtt cgcggtcgga ttgttccgag
     2581 agacgttttt cccagagagg gaagaaatct tcttcgcggc agaggaagag cgggtgttcg
     2641 gtgacggaac cgatcaccgt ggtttttatc cgggggtcga tgcaatagag gttgtccgcc
     2701 agatcggacg acatgaccca gacggggcgc cggcccgtgg ggtggagacg gagccactcg
     2761 ccgacgtcgt ggacggcgtc cgtgaatctc cggcccgccc cggtctcttt catccggtga
     2821 aagtagtcgg ccaacaggcg cgcctcgcca taacagaaag agacgagaaa aacgatcgtc
     2881 agaccccgca aaaaccgacg ctcccggaga agggcgaaaa gttgcgaaaa ggccaacgcc
     2941 agaacgatct gcgggaaagg gaaaaagaca aacatgtatt gggaatcgcg cggggtgcct
     3001 tggatccggg agacgtggga gacgacggac atgatctgca tgacgacaaa aagtgagagc
     3061 agcccgagca cggtttttcg ggaaggaaga tttttccgaa aagccggcag gagggcgcaa
     3121 aaaataaaag ccgcccagaa ggccgcgggg taaagggaat tccccggagg ggacgcctgg
     3181 aagaagtttt catagggtcg atgccccagc aaaaaaaggt ggaacccccc gatggcgcga
     3241 tggaggacgt cccacgagat cgacggggaa tggccggagg cggccaggtg ttgcaacgcc
     3301 aggggaaggg tcggggtgtc gatccggagt tcggtgcgcc acatcaggaa cgtgctggcg
     3361 aggaacccca gcccggcgat aaagaggggt ttgaggagtt tgttccagac gccggggagg
     3421 agttgcgtcc ggatgtccca gagggcgagc agaaagacgc ctccgaaagc ggccccatac
     3481 cagatgaaga aaaaccgggt gcccagtccc agtcccaaaa ggaaggtcgc caggaacagc
     3541 gggatcgttt tttgggtgag ccaccaacga tggaggaagt aaagagagct gatggagaaa
     3601 aaagtcatgt aggagacctg gtaattcccg gatttcaccc ccatcagata cgcggggtgg
     3661 atgaccagga acagaagggc gaggagtccg gctttgggtt tgaaggccgc gttggcgaaa
     3721 cgatacgtca ccagcagtgt cagcccgccg aagacgatcg gccacagccg gacgatcgtc
     3781 caggacgtgc cgaaaacgta gaagaagggc atcatcaagt agcgggtgag gcagccgttg
     3841 tagagatgga aattgaaacg gaaaagggag aagggaaggc cgttcctcag ttcgtcggcc
     3901 cagcgggcgc cggccgcctc atcggctagg ccgtcgacaa tgtagccggg ggaagtgaaa
     3961 taggagaggg tgaggacgaa atagaggaag aggaggaacg cggggagaag gggcgcgggt
     4021 tttttgaccg cctcgaggtt ccctcggagg gagaaatccc actccaggcg ccacctcatg
     4081 tggatcgagg tcccggctgt ttcaccgatg cgggcattcg ccgtgggggt ggttttaagc
     4141 cgatttgttt cggacctgct caagcatggt ttttagcatg tccgagagag gtggggcgcc
     4201 gaggtctttc ccctcgcgcg tgcggacgga gacggtcttg ttctcggcct cgcgcgcgcc
     4261 caccacgagg agatagggga tcttctgcag ggtggcctcg cggatcttgg cgccgatttt
     4321 ggaggcggag tcgtctttgt gcacgcggag cccggcctct ttcagccggt tctggacctc
     4381 ctcggcgtac gcggcgaatt tgtcgctgat gggcaggacc gccgcctgca ccggcgccag
     4441 ccacaacggg aacgcccccg catagtgttc gatcatgacg ccgaaaaagc gttccaggga
     4501 gccgaacagg gcgcgatgga tcatgaaaac ttccgtctct ccgcccttct cgtcgcggta
     4561 agtgatgttg aaccgtttgg ggttgttgaa gtccacctgg acggtggaac actgccattc
     4621 ccggccgagg ctgtccttga tcttgaggtc gatcttcggt ccgtaaaaaa cgccttcgcc
     4681 gggatcgacg gcgtatttca gattcatccg ctccagggct ttcttgaggg cgttttcggc
     4741 atgcgcccat ccttccagcg tgccggaata tttgtccggg cgggtggaga gcttgagggc
     4801 ataatcggaa aaaccgaaag tcttcaagac gtccgtgatg gtctgcatga tgttgaccac
     4861 ctcgtcctcc acctggtcgg gccggcagaa gatgtgggcg tcgtcctggg tgaacccgcg
     4921 cacccgcatc agcccgtgca gcacgccgct gcgttcgaac cggtagacgg tgccgaactc
     4981 cgccaggcgg agcggcagtt cgcggtagga atgcagttcg gacttgtaga tgaggatgtg
     5041 gccgggacag ttcatgggtt tcagctggaa atcctggttc tccagcttca tgggcgggta
     5101 catgttctcc gcgtaatagt cgagatggcc ggaggttttc cagaggtcga tgcgggccac
     5161 gtggggcgtg tacacgaact gatacccttt ggccccaagg aaatccttga ggtaatcctc
     5221 ggcgatgcgg cgcacgatgg cgcctttggg gagccaatag atgaaccccg agccgccctc
     5281 ttcgtgctgg atggtgaaaa gcccgaggcg cggccccagt
//
LOCUS       DAOTFD010000185         5227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11442_length_5227_cov_3.991686, whole genome shotgun sequence.
ACCESSION   DAOTFD010000185 DAOTFD010000000
VERSION     DAOTFD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11442_length_5227_cov_3.991686"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..144
                     /locus_tag="P5079_09345"
     CDS             40..144
                     /locus_tag="P5079_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRY30224.1"
                     /translation="MAEAFLGHKEGETVVVKLAAGPVTYQVLKVSREI"
     gene            170..1114
                     /locus_tag="P5079_09350"
     CDS             170..1114
                     /locus_tag="P5079_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30225.1"
                     /translation="MKAFVAAAGPFALPVAEELVRACWGIETLFYDARSGADREAFEK
                     FAKQVSFPVRPVPGGDWEKFWQSLAAPSEVLLFLIGAVEPETPLRFSSVYSLQAGLLP
                     GQAGPDPLPWALWKGEPATGLTLYRRGARPYSGDLLFQTSCPIADSDDVGALEEKLKE
                     LAIRLVREFWESSTKAQLRPKESFAAAVVNPPWTGGAIPWANPTRDVHNFVRASARPG
                     AGAFTYLKGRKLTVRKTTPLAPRGEIVSCRPGEIFSYDPFRVCTGDGMLRLERVRWEG
                     EEEMDGTAFARTRAIEPGLMMGQDLEAAAPAVPSGNNA"
     gene            1127..1567
                     /locus_tag="P5079_09355"
     CDS             1127..1567
                     /locus_tag="P5079_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HRY30226.1"
                     /translation="MAEVIKKLKKNSSEEVWITVTDYKERELIDIRVYYRSIDDMEMK
                     PTKRGISIPLAKVAELLAAIEKINPFAKIGEPVGKIDKSETEMIYVTLKEFKGHKLVD
                     VRTYFRPQLGEELVPSQKGVTFRYDTAVLEEVKEALRRAQTMAK"
     gene            1657..2028
                     /locus_tag="P5079_09360"
     CDS             1657..2028
                     /locus_tag="P5079_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30227.1"
                     /translation="MNWIYWVVGLFWIIDGFIYFVFPAFARGMFMRAFEEGRYVAASL
                     GPAMTGVLLWSAAMSSPHAWTLRVLALLSLVQAAYLFRALRGTGPRLIEKIVTFPNRY
                     FRVWGLVLTLLGLFLIGIRPW"
     gene            2028..2660
                     /locus_tag="P5079_09365"
     CDS             2028..2660
                     /locus_tag="P5079_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRY30228.1"
                     /translation="MDPLGLFKGGKKPPERRQKRRSLRVFRRDRRLDRWFYGVALALA
                     AVLYFYHVPSRKSDLSPASQDYWLVGTSSGTFCSDPAALVRASVRETVDGDTLRVMWE
                     GRPRFLRYYGVDTPERDEPCYREATERNRQLAGNTVLLGFDGRREDSHGRLLAYVFTE
                     DGLFIDAQLVAEGWGRAWRRDGRFHEYVARLEENARAAGTGCLWERTGKR"
     gene            2657..2956
                     /locus_tag="P5079_09370"
     CDS             2657..2956
                     /locus_tag="P5079_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsJ"
                     /protein_id="HRY30229.1"
                     /translation="MKLFVDDWRSAPEGWETARSVPEAIERLRVGKVEEVSLDYVIGD
                     DFENNFSPVARFIADMPESQRPKRVIIHTSSSEGARLLRRILEGRVERVLGSYDA"
     gene            2946..3356
                     /locus_tag="P5079_09375"
     CDS             2946..3356
                     /locus_tag="P5079_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30230.1"
                     /translation="MTPEKDRPQTVLVVDDDPPMNELVKLVLETRGYRVLAVSDGKVA
                     VETIRSRRPDLVVLDVMLPEISGLEICEMVKGDPEFKDVKVVMLSAKSLNVDKLRARG
                     KGADAYLTKPVRLAELLDTVNGLLVPSGIPGAKP"
     gene            3353..3733
                     /locus_tag="P5079_09380"
     CDS             3353..3733
                     /locus_tag="P5079_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30231.1"
                     /translation="MKKEKPRVVIVEEENTFSGLAGYLPKFRYDVRALSFSEEAAGQA
                     AGWKPHVVVSNLPGAAALKICRGLRGGPGGPALRLLCLVGSRAEMEFLKAFQAEANAY
                     LVKPVDELAFTTQIDELFEERSAG"
     gene            3730..4539
                     /locus_tag="P5079_09385"
     CDS             3730..4539
                     /locus_tag="P5079_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRY30232.1"
                     /translation="MKPVSEPVKQRLYAVLEGGGADAASRAVGVSLMTLIVLNVLSVV
                     LETVESLQVAYGGLFRAFEVFSVVVFSAEYVLRMWSCTADERYARPVWGRLRFMLGPL
                     ALVDLAAILPFYLPLVMRLDLRVMRAVRLVRLFRILKMGRYSEALGVFRKIFWSKKEE
                     LLITVFAAGVMLVLVSSLMFFIENEAQPKVFSSIPAAMWWGVATLTTVGYGDIYPITT
                     AGKILGTLSAVFGIGMFALPAGILGSAFVEEMHRRREKSKICPHCGKEIEE"
     gene            4536..>5227
                     /locus_tag="P5079_09390"
     CDS             4536..>5227
                     /locus_tag="P5079_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30233.1"
                     /translation="MRRPFGKGSLRVGGALCATVLFAALTLRAASGQVAFEEGWKWYL
                     HRRSQNAPGEELLDILSTLKKKYTGTGVDISQVDREMGRYAPPSSAAPEDGFVDGWKW
                     YEHRKAAGAAPKELVDILETLKKKYEGSGMDLSRVERELAYYHFERSVVKPADGASTE
                     KDEPLSVPAAPARRPSAPPSAVSEWEQKDLSPDEALLRRCAADAASNDTTRRLKAVQE
                     LKGVRDPRAVDIL"
BASE COUNT          905 a         1469 c         1824 g         1029 t
ORIGIN      
        1 gacggaccca ccatagagga tccggacccg ctctccccgc tggccgaggc gttcttgggc
       61 cacaaggagg gagagacggt ggtcgtcaaa ctcgccgccg gcccggtgac gtatcaggtg
      121 ttgaaggtgt cgagggaaat ttgacgccga cgggccgcgg ggggagacga tgaaggcgtt
      181 tgtggcggcc gccgggccgt ttgctcttcc ggtggcggaa gaattggtgc gggcgtgttg
      241 gggaatcgaa accctttttt atgacgcgcg ttccggggcc gaccgggaag cgtttgagaa
      301 gttcgccaag caggtgtcct tccccgtccg tccggtcccc ggcggcgatt gggaaaaatt
      361 ttggcagagc ctggctgcgc cgagcgaggt cctccttttt ttgatcggcg ccgtggagcc
      421 ggagacgccg ctgaggtttt ccagcgtcta ctctctgcag gcgggtcttt tgccgggcca
      481 ggcggggccg gacccgctgc cttgggccct gtggaagggg gaacccgcca cgggactgac
      541 gctctaccgt cgaggggccc gtccctacag cggcgatctg ctgttccaaa cctcctgccc
      601 gatcgcggac agcgacgacg tcggcgcgtt ggaagaaaaa ctcaaggaac tcgccattcg
      661 cctggtgcgg gaattctggg agtcgtccac caaggcgcaa ctgcggccga aggagtcctt
      721 cgccgccgcc gtggtgaacc cgccttggac cggcggggcg atcccgtggg ccaaccccac
      781 ccgcgacgtc cacaatttcg tccgggccag cgcgcgtccg ggcgccggcg ccttcaccta
      841 tctcaaaggc cggaaactca cggtgcgcaa gaccacgccg ctcgccccca ggggggagat
      901 cgtttcttgc cgcccgggag agattttttc ctatgacccg ttccgcgtct gcaccggtga
      961 cgggatgctc cgtctggagc gcgttcggtg ggagggcgag gaggagatgg acggcaccgc
     1021 gttcgcgcgc acgcgcgcca tcgagcccgg gttgatgatg ggacaggatt tggaggcggc
     1081 cgctccggcg gtcccctccg gaaacaacgc ctgaaaggag caaaacttgg ctgaagtcat
     1141 caagaagctg aagaagaact caagcgaaga ggtctggatc accgtcaccg actacaagga
     1201 gcgcgagctc atcgacatcc gcgtctacta ccgttccatc gacgacatgg agatgaaacc
     1261 caccaagcgc ggcatcagca tccccctcgc caaggtcgcg gaattgttgg cggcgatcga
     1321 gaagataaat ccgttcgcga agatcggcga accggtcggc aagatcgaca agagcgagac
     1381 ggagatgatc tacgtcaccc tgaaggagtt caaggggcac aagctcgtcg acgtgcggac
     1441 gtatttccgg ccgcagctcg gcgaggaact cgttccgtct caaaagggag tgacgttccg
     1501 ctacgacacc gccgtcctcg aggaagtgaa ggaagcgctc cgtcgggcgc agacgatggc
     1561 gaaataggga cggtctcctc gggttcggca tctccttcgc cccggcggtt cgccgacggg
     1621 gattgtggcc tacggaacga gtgagaggag cgagcggtga attggatcta ttgggtcgtc
     1681 ggcctttttt ggatcattga cggtttcatc tattttgtct ttcccgcgtt cgcgcggggc
     1741 atgttcatgc gggcttttga agaaggccgc tatgtcgcgg cgagcctcgg cccggccatg
     1801 acgggtgtgc tcttgtggtc ggcggctatg tcgtcccctc atgcctggac gctgcgtgtg
     1861 ttggccctcc tctccctcgt ccaggcggcc tatcttttcc gcgccctgcg gggcaccggt
     1921 ccccgcctca tcgaaaaaat cgtgacattt ccgaaccgct atttccgcgt ctgggggctt
     1981 gtcttgaccc tcctgggcct cttcctcatt gggatccgac cgtggtgatg gatccgttgg
     2041 ggctcttcaa gggagggaag aaaccccctg aacgtcgtca gaaacggagg tcgttgaggg
     2101 ttttccggcg tgaccggcgg ctggaccgct ggttctatgg ggtggcgctg gccctggcgg
     2161 cggtcctgta tttctatcac gtgccgtcga ggaagagcga tctttctccg gcttctcagg
     2221 attattggct ggtgggcacc tccagcggca cgttttgctc cgaccccgcg gccttggtcc
     2281 gggcgtcggt ccgcgagacg gtggacggcg acacgctgcg cgtgatgtgg gagggccgcc
     2341 cgaggttcct ccgctactac ggtgtggaca cgccggaacg ggacgaaccg tgttatcggg
     2401 aagcgacgga acgcaatcgg caattggcgg ggaatacggt gcttttgggc tttgacggcc
     2461 gccgcgagga ctcgcatggc cggcttctgg cgtatgtttt taccgaagac ggtcttttca
     2521 tcgacgcgca attggtggcg gaggggtggg gtcgcgcttg gcggcgtgac ggccgatttc
     2581 atgaatacgt ggcgcggctg gaggaaaacg cccgagcggc cgggacgggt tgcctgtggg
     2641 agaggaccgg gaaaagatga aactttttgt ggatgactgg cgttcggctc cggaagggtg
     2701 ggaaacggcg cgcagcgtgc cggaagccat cgagcggttg agggtgggaa aggtggagga
     2761 ggtcagcctg gactacgtca tcggcgacga tttcgagaac aatttcagtc ctgtggcccg
     2821 cttcatcgcc gacatgccgg agtcccagcg cccgaaacgc gtgatcatcc acacctccag
     2881 cagcgagggg gcgcgtcttt tgcggcgcat cctggagggg cgcgtggagc gtgttctcgg
     2941 atcctatgac gcctgaaaag gaccgcccgc agaccgtcct ggtggtggac gacgatccgc
     3001 ccatgaacga actcgtcaag ctcgttctgg agacccgggg ttaccgggtc ctcgccgtct
     3061 ccgacggcaa agtggcggtg gagaccattc ggagccggcg gccggacctc gttgtgttgg
     3121 acgtgatgct gccggagata agcggtttgg agatttgcga gatggtgaag ggggacccgg
     3181 agttcaagga cgtcaaggtg gtcatgctct ccgccaaatc gctgaacgtg gacaagttgc
     3241 gggccagggg gaaaggggcc gacgcctatt tgacgaaacc cgtccgtttg gccgagctcc
     3301 tggatacggt caacggcctg cttgttccgt cgggaatccc gggggcaaaa ccatgaagaa
     3361 ggaaaaaccc cgcgtcgtca tcgtggagga ggagaatacg ttttccggat tggcgggtta
     3421 tctgccgaaa ttccgctacg atgttcgcgc gctgagtttc agcgaggagg cggccggaca
     3481 ggcggcggga tggaaaccgc acgtggtggt gtcgaacctc cccggggcgg ccgctttgaa
     3541 aatctgtcgt gggttgcggg gcggtccggg cgggccggcc ctccgcctcc tgtgcctggt
     3601 cggctcgcgg gcggagatgg aattcttgaa ggcgtttcag gcggaggcca acgcttacct
     3661 ggtcaaaccg gtggacgaac tggcgttcac cacccaaata gacgaattgt tcgaggagcg
     3721 ttccgccgga tgaaacccgt gtcggagccg gtcaaacagc ggttgtacgc ggttctggaa
     3781 gggggcggtg ccgatgcggc cagccgggcg gtcggcgtct ccctgatgac gttgatcgtg
     3841 ctgaacgtgc tgagcgtggt gttggagacc gtggagtctt tgcaggtggc ctacggcggt
     3901 cttttccggg cgttcgaggt tttttcggtc gttgttttca gcgcggaata cgtcctgcgg
     3961 atgtggtcgt gcacggcgga tgaacgctat gcgcggccgg tgtggggccg gctccggttc
     4021 atgctcggcc cactggcgtt ggttgacctg gcggcgatac ttcccttcta cctgccgctg
     4081 gtgatgcgtc tcgatctgcg cgtgatgcgc gccgtgcggt tggtgcggct gttccgcata
     4141 ttgaagatgg ggcgttattc ggaggcgttg ggcgtgttcc ggaagatttt ttggtctaaa
     4201 aaagaggaac ttctgattac ggtgtttgcg gccggggtga tgctggtgtt ggtgtcgagc
     4261 ctgatgttct tcattgagaa cgaggcgcaa ccgaaggttt tttcgagcat cccggcggcc
     4321 atgtggtggg gcgtggcgac cctgaccacc gtaggatacg gggacatcta tcccatcacc
     4381 accgcgggaa aaatattggg cacgctctcc gcggttttcg gtatcggcat gttcgccctg
     4441 cccgccggca tactgggctc cgcgttcgtc gaggagatgc atcgtcgtcg ggagaagtcg
     4501 aaaatctgtc cgcattgcgg aaaagagatc gaggaatgag gcgcccgttc ggaaagggat
     4561 ccctgcgcgt cggcggggcg ctgtgcgcga cggtgctgtt tgcggcgctg acgctccggg
     4621 ccgcttcggg acaagtggct ttcgaagagg ggtggaagtg gtacctccat cgtcgttcgc
     4681 agaacgcccc cggcgaggag ttgttggata tcctgtccac cctgaagaag aaatacaccg
     4741 gcacgggagt ggatatctcc caggtggacc gggaaatggg ccggtacgcc cccccgtcct
     4801 ccgccgcacc ggaagacggg ttcgtggacg gctggaaatg gtatgaacat cggaaggccg
     4861 ccggcgccgc ccccaaggaa ttggtggaca tcctggaaac gctgaagaaa aaatacgagg
     4921 ggtcggggat ggatctgtcc cgggtggagc gggaactggc gtattatcat ttcgagcggt
     4981 cggtcgttaa gcccgccgat ggagcgtcga cggaaaagga cgagcctctt tccgtccccg
     5041 ccgccccggc ccgccgtcct tccgcgccgc cctccgcggt ttcggaatgg gagcagaagg
     5101 acctttcccc cgacgaggcg ctgctccggc gatgcgcggc ggacgccgcc agcaacgaca
     5161 ccacccgtcg tttgaaggcc gtgcaggagt tgaaaggcgt gagggatccg cgggcggtgg
     5221 acatcct
//
LOCUS       DAOTFD010000186         5195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11537_length_5195_cov_3.980350, whole genome shotgun sequence.
ACCESSION   DAOTFD010000186 DAOTFD010000000
VERSION     DAOTFD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_5195_cov_3.980350"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="P5079_09395"
     CDS             complement(<1..981)
                     /locus_tag="P5079_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30234.1"
                     /translation="MPILSLYPAAARGGRLLCHRRRWAVLLAAFLIQDLYLPLWAEYR
                     LMDKSPEEDLQLTEPVSLPPEPPPEETLFHFDQQRWNKARDAIDRGEEPGAAPKKKEK
                     EAVAVSTAPVTPPPEEPGLSVELPYESGLSISGRKLIALKLKETRYKSAKRAQELNVN
                     KVQREFEMKQELQVRIKGKVGRKITVNVDFDDTKEDKRDISVVYQGDPDEFVQEAAFG
                     DITLSLPSTEFVSYNKQLFGVRGKLKYKRAQLMAIGSRTKGVTETKRFTGNTQFERKE
                     ISDTAYIQRRYYNLAFDTGHAPLQVGSEQIWLDNRVPTDNTPNTQPFTAED"
     gene            1547..2629
                     /locus_tag="P5079_09400"
     CDS             1547..2629
                     /locus_tag="P5079_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY30235.1"
                     /translation="MILPRYLLRQFFPPFFFGLALFSGVLLMDKLFDIVDLLVNKGVS
                     LSVSLRIFLYFLPTVLTLTVPMAVLLACLLTFGRLSEENEITAMRASGLSFTQILWPP
                     LAIALVLCAVLAPFNTLVAPRAINAFRSLYHEIVTTDPLIKIEPRQFINLRDFRLYVD
                     KAAPDRQSLQDVQVYQITTDFWQRIYARRGFAKVETNRLFLRLEDGQLERAPRGRSQD
                     MMHMQFMEYTLSVPFGGPESSRDQSWREMTMTQLHTEIQTRKKLGVPAGPVRAEWNLR
                     FAVAFSPLALAMLGIPLGITLQRGGRGVGFGASLAVVFAYYLLLILGLNLAEKDTLPA
                     LPSLWMANVFTMAVGVFLYRRRVLKS"
     gene            2626..3714
                     /locus_tag="P5079_09405"
     CDS             2626..3714
                     /locus_tag="P5079_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY30236.1"
                     /translation="MNRITKYFLEEFLQPLFVSYGALVILVLVAELMEQMDKFISGKA
                     GVWMVMQYLLSLLPMRSMEVLPVAVLLATLFSLGNLSRRQEITAAMSGGIHPWRCVKL
                     LLATGALLSVLTLALSEWVVPFANRHAKKIWKMDIRHLGSFRQTRFDNLTMAGQGGVF
                     YSIGTLDVEKGILEDLVVEWFENGRPRRQIQAEKAEWRPGEGWVFNNGVERIFSDDAF
                     ALATQTPFSEKTVALEASPEDLIPKEPEAEEMNYKQYNRHIRRLRRLGVSTRRQEVEL
                     HMKLAFPLTSFIVLLLGIPFGFQKTGGKVKAIGMALAVAFFYFGLMEVGRAVGQKSWC
                     PTILAAWLANLVFLGVGGWLFVRMRKLS"
     gene            3804..4148
                     /locus_tag="P5079_09410"
                     /pseudo
     CDS             3804..4148
                     /locus_tag="P5079_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(4277..>5195)
                     /locus_tag="P5079_09415"
     CDS             complement(4277..>5195)
                     /locus_tag="P5079_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30237.1"
                     /translation="GITTADGSNPPHLDAWCKKFLGFASVQSLSGTVSHSPSETTRTG
                     VNQLAIPVGGTSEYFLVEYRLRSSAGSYDKGLPMDGLAVWHIDDAIALNTTQLANNTV
                     NSPSQNGFNHLGIDLVEADGTAANPQNSDLGIGDAYADGQTFAAPYSNSFSGQPSGVT
                     IANIAGVGTASVNSSVAFIQATTALTVAKTVNYPNPAGDTKKYPVRAGAPAGTITTFV
                     AQFSRPVESSELQLDIYNLRGDRIKGISGPEITLKIGAGEPSSDYKWVYEYDWNGKND
                     SGDEIVSGVYLYRFKAQEEVKTGKVMIVR"
BASE COUNT          953 a         1499 c         1530 g         1213 t
ORIGIN      
        1 gtcctcggcg gtgaaaggct gggtgttggg ggtgttgtcg gtgggcacgc ggttgtcgag
       61 ccatatctgt tcggaaccca cctgcagagg cgcatggccg gtgtcgaacg ccaggttgta
      121 atagcgccgc tggatgtagg cggtgtcgga gatctccttg cgttcgaact gggtgttgcc
      181 ggtgaatcgt ttggtctcgg tcacgccctt ggtgcggctg ccgatggcca tgagctgggc
      241 gcgtttgtat ttgagtttgc cgcgcacccc gaaaagctgt ttgttgtagg agacgaactc
      301 cgtcgacggg agcgagaggg tgatgtcgcc gaaggccgct tcctgcacga attcgtcggg
      361 gtctccctgg tagaccacgg agatgtcgcg cttgtcctcc ttggtgtcgt cgaagtccac
      421 gttgacggtg atcttccgcc cgacttttcc cttgatgcgg acctgcaatt cctgtttcat
      481 ctcgaactcg cgctggactt tgttgacgtt gagttcctgg gcgcgtttgg cgcttttata
      541 ccgcgtctcc ttgagcttga gggcgatgag ttttcgcccg gagatgctca ggccggattc
      601 atacggcagc tccacggaga ggccgggttc ttcggggggc ggggtgacgg gggcggtgga
      661 aaccgccacc gcttcttttt ctttcttttt gggggccgcg ccgggttctt cgccgcgatc
      721 aatggcgtcg cgggctttgt tccagcgctg ttggtcgaaa tggaagaggg tctcttcggg
      781 agggggttcg ggcggcaggg aaaccggttc ggtcaactgc aaatcttctt ccggggactt
      841 gtccatcagc cggtattcgg cccagagcgg caggtagagg tcttggatga ggaaagcggc
      901 gaggaggacc gcccagcggc ggcggtgaca aaggagccgc cccccccggg cggcggcggg
      961 ataaagagac aatattggca caaataaaaa agacatccgg cgcattacaa gaagtccctc
     1021 atccctgtgt tttcatccca aggggtcgtc cgcgcccccg gaagatgtct ttcggcaacc
     1081 cagccgcctt aaaagaaagc tcttcaaaac atttaagagt caagatattt aagaatttcc
     1141 ttgatgtata ttcttaactc ctctttcttc gttaggcctg tggctttgcc agccgccaac
     1201 gcgggcatcc cttcccggag ccccccttgg ctgaccggcc ccccgcctgg gaagtccgtt
     1261 cctcgctttt cagaaggaac cgccccgaaa acggggcgaa tgaggcggag agcgtccttg
     1321 cctttcggca agtttgcccc tccaaaccag ggtgagtccc cgaaaatgga ggaaccccta
     1381 cggggtggga cacctcttca ggagggaccc cgccttccgc aaggtttttg tcgttttgtc
     1441 gctatgaaaa gcttatcccg ccgacctgtt tctgtcaata gaaacttcca agaagcttct
     1501 atgcaaagaa ctggccagtg ggaaagggga tttgtataat caccccatga ttttgccgcg
     1561 ctacctgctc cgccagtttt tccctccgtt ttttttcgga ctcgccctgt tttccggcgt
     1621 cctgctcatg gacaagctct tcgatatcgt ggatttgctg gtcaacaagg gcgtttctct
     1681 gtcggtttcc ctgcgcattt tcctctattt ccttcccacc gtgctgacct tgaccgtgcc
     1741 catggcggtg ttgttggcgt gtctgctgac cttcggacgg ctttccgaag agaatgagat
     1801 caccgccatg cgggcctccg gcctctcgtt cacgcagatc ctgtggcccc ccttggccat
     1861 cgccctcgtc ctctgcgccg tgctggcccc cttcaacacc ctcgtcgccc cccgggccat
     1921 caatgccttc cgcagccttt atcatgagat cgtcaccacg gatccgttga tcaagatcga
     1981 accgcggcag ttcatcaact tgcgggactt ccgtctctat gtggacaagg ccgcccccga
     2041 ccggcagtcc ctgcaggacg ttcaggtcta tcagataacg accgactttt ggcaacgcat
     2101 ctacgcccgc cggggtttcg cgaaagtgga gacgaaccgc ctcttcctcc gtctggagga
     2161 cggccaactg gagcgcgccc cccggggccg ttcgcaggac atgatgcata tgcaattcat
     2221 ggagtacacc ctttccgtgc cgttcggcgg tccggaaagc agccgcgacc agagctggcg
     2281 ggaaatgacc atgacgcaac ttcacaccga aatccagacg cgcaaaaaat tgggcgttcc
     2341 cgccgggccc gttcgggcgg agtggaacct tcggttcgcc gtggcgtttt cccccctggc
     2401 gttggcgatg ttggggatcc ccctcggcat caccctccag cgcggcggcc gcggcgtcgg
     2461 tttcggcgcc tccctcgccg tggtgttcgc ctactacctt cttctcatcc tggggctcaa
     2521 cctcgccgag aaggacaccc tgccggccct gccttccctc tggatggcca acgttttcac
     2581 catggccgtg ggcgtctttc tttaccgtcg acgggtcttg aaatcatgaa ccggatcacc
     2641 aaatatttcc tggaggagtt ccttcagccg ttgttcgtga gctacggcgc cctggtgatc
     2701 ctcgtcctgg tggccgaact catggaacag atggacaaat tcatctccgg caaagccgga
     2761 gtgtggatgg tgatgcagta ccttctttcc cttctgccca tgcgcagtat ggaggtcctg
     2821 ccggtggcgg tgcttttggc cacgcttttt tccctcggga acctgtcccg ccgtcaagag
     2881 atcaccgccg ccatgtccgg cgggatccat ccgtggcggt gcgtgaaact cctcctggcc
     2941 accggcgccc tcctcagcgt cctcaccctg gccctcagcg aatgggtggt cccgttcgcc
     3001 aaccgccacg ccaagaaaat atggaagatg gacatccgac atctcggctc gtttcgtcag
     3061 acccggttcg ataacctcac catggccggc cagggcggcg ttttttattc catcgggacc
     3121 ttggacgttg aaaaggggat cttggaggac cttgtcgtgg agtggtttga aaacggacgt
     3181 ccccggcgcc agattcaggc cgagaaagcc gagtggcggc ccggagaagg gtgggttttc
     3241 aacaacggcg tggaacgtat tttttcggac gacgcctttg ctttggcgac ccagacgccg
     3301 ttctcggaaa agacggtggc gctggaggct tccccggagg atttgatccc caaggaaccc
     3361 gaggcggagg agatgaacta caaacaatac aaccgccaca tccggcgctt gcgccggctc
     3421 ggcgtctcca cgcgccgcca ggaggtggaa ctccacatga aactggcgtt tcccctcacc
     3481 agcttcatcg tgcttttgtt ggggatcccc ttcggttttc aaaaaacggg cggcaaggtg
     3541 aaggccatcg gcatggcgtt ggcggtggcg tttttctatt tcgggctgat ggaggtgggc
     3601 cgcgccgttg gccaaaaatc ttggtgcccg acgatcctgg cggcgtggct ggcgaatttg
     3661 gtttttttgg gcgtgggcgg ctggttgttc gtgcggatga ggaaactttc ttaaaaagta
     3721 tgaagttgga aggtggaagt aagaagtaac gataagaaaa aagacgttcg gatgggcgga
     3781 gcattacata gaaatcaaaa cccatgaacg atctgaagga ccgtacaaaa gattttgcgt
     3841 ttgccgtttt ccggtcctgc gataaatcgc cgcaacgaca ggtttattgg atcatcggca
     3901 aacagttcat gcggtcggcc gcatccatcg gcgccaatta caggtcggct tgacgcggaa
     3961 aatcgaaggc ggatttcacc gcccaatagg cgattgtggt cgaagaggcg gatgaaacgg
     4021 tttattggct ggaactgctg gaggcgttgg aaagtcaaag cgagaagctt gttcgactaa
     4081 gaaacgaagc cagtcaactg attgctattt tcgcagctgc ccttaagaaa tcccgcttca
     4141 ataaatagct gtttggccac ttttcgcctt ttgtcgccga accacttctg attcaagaat
     4201 atccgtattt tatctcaacc gtttcccact ttttacttca aacttcgtaa ttgttcgtcg
     4261 cctgcactgc ttactcttat cggacgatca tcacttttcc cgttttgacc tcctcctggg
     4321 ctttgaaccg gtacagatag acccccgaaa cgatctcatc cccgctgtcg ttctttccgt
     4381 tccaatcgta ttcatagacc cacttgtaat ccgatgacgg ttcccccgcc ccgatcttca
     4441 gcgtgatctc cgggccggag atgcctttga tgcggtcccc ccgcagatta tagatgtcca
     4501 gctgaagctc cgaactctcc acgggccggc tgaactgggc cacgaaggtg gtgatggtcc
     4561 cggcgggcgc tccggcgcgc accggatact ttttcgtgtc gccggccgga ttggggtaat
     4621 tcaccgtttt cgccaccgtc aaggcggtgg tggcttggat gaaggccacg ctggaattga
     4681 cggaagcggt ccccacgccg gcgatgttgg cgatggtgac ccccgaaggt tgcccgctga
     4741 aagagttgga ataaggcgcg gcaaaggtct gcccatccgc gtaagcgtcc cctatcccca
     4801 ggtcgctgtt ttgcggattg gcggccgtgc cgtcggcctc caccaggtcg atccccagat
     4861 ggttgaagcc gttctgggag ggcgaattga ccgtgttgtt ggcaagctgg gtggtgttga
     4921 gggcgatggc gtcgtcaatg tgccacaccg ccagaccgtc catgggcagc cccttgtcgt
     4981 aacttccggc ggaggaccgc agacggtatt ccaccaggaa atattcgctt gttcccccca
     5041 ccgggatggc gagttggttg acgccggtcc gggtcgtttc cgagggcgag tgggagacgg
     5101 ttccggagag ggactggacc gacgcgaagc ccagaaattt tttacaccac gcgtccaaat
     5161 ggggcgggtt ggacccgtcc gccgtggtta tgccg
//
LOCUS       DAOTFD010000187         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11540_length_5193_cov_4.002530, whole genome shotgun sequence.
ACCESSION   DAOTFD010000187 DAOTFD010000000
VERSION     DAOTFD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11540_length_5193_cov_4.002530"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="P5079_09420"
     CDS             <1..622
                     /locus_tag="P5079_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRY30238.1"
                     /translation="LPAPDPEPPAHAAPLLGLLPGSRRGEIRRHLGLFLQTADRLTQD
                     LPNLRCALFAAPSIPDDFYNPLLARHPHRTFPLEIVREEHYAQRAQLDLALTCSGTAT
                     LENALLGIPMLVVYKMSWITYGLARALIRVEHIAMANILAGKKLVPELIQDQASPALL
                     AQEALRLLKDPQERLRVRRELLSLRQQLGGPGAADRAAAEIIQALG"
     gene            627..2357
                     /locus_tag="P5079_09425"
     CDS             627..2357
                     /locus_tag="P5079_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY30239.1"
                     /translation="MQTPVVRRLIHYFRGHLPMLTVALVAMAAVAALTAAAMWILKQV
                     VDKALTAGDLTALQEIVLLVVVLFAMKAVLSYVHDYLTAHVGQAIVRQLRDDVYAHLH
                     TLSMDFFTLNDSARLISRLTNDAQLVQNALTKIPVMIVRDGLTILCLTGFLLYLHWKF
                     ALLAFTILPVSGYLIARFGKSLRKTARLGQAKMSDLYLQIQETITGAPVVKAYLRENY
                     ERDRFRRENHAYFTITMKNARVESLSSPVMEFLGAIGMAAILWLGGLDVINGVWTTGA
                     FFAFVGSALSLYQPVKNFSRSNATLQLALAGAERIFEILDTQPTVMEKPGAPPLPPFT
                     TALKFENVNFHYRPDRPVLKNINVTVSKGEIVALVGPSGSGKTTMAALLLRFYDPVSG
                     RVAFDGHDLRDVSLAGLRGQIGLVTQETLLFNDTVRHNIGYAKANATEQELIAAARAA
                     NAWEFIEKLPQGLDTLVGERGVLLSGGQKQRLAIARAILKNPPILVLDEATSALDAES
                     ERLVQEAVEQLMKNRTVIVIAHRLATIKNADRIVVLEHGRVAEMGTHTELLSKKSLYH
                     RLYELQLLQE"
     gene            2365..3084
                     /locus_tag="P5079_09430"
     CDS             2365..3084
                     /locus_tag="P5079_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRY30240.1"
                     /translation="MPLPLTFSLISVFATLGGGWLLLGRKEWTLKYLWRFLAFGSGVI
                     FGITFLHLIPESWRLDSRWGAGSLLVAFVTFYALEEFVVAHHACGELTEDCHEHKFGY
                     GALVALFLHSLSDGLAMAFSFLSSTALGVGVSTAVIVHKFSDGMTLSSLFLNQGFSRK
                     KIWALTSVLSIATPLGLMIGLSATGLITPTVLAVLLGLAAGSFLYVAMADILPRLHHN
                     KDIHSWILFLVGIGLSALYPH"
     gene            3104..3304
                     /gene="thiS"
                     /locus_tag="P5079_09435"
     CDS             3104..3304
                     /gene="thiS"
                     /locus_tag="P5079_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRY30241.1"
                     /translation="MTIQVNGEKRQVPDGATVQSLLESLGMGAGRVACELNLNIVKRA
                     DFPSTRLKEGDSVELVQMIGGG"
     gene            3645..4445
                     /locus_tag="P5079_09440"
     CDS             3645..4445
                     /locus_tag="P5079_09440"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRY30242.1"
                     /translation="MNDKPLVIAGKEYTSRLIVGTGKYKTLPLMKEALTASGAEMVTV
                     AIRRVNLQDKSPESFWNHIPEGMTILPNTAGCYTAGDAVRIARLAREVLQTPLIKLEV
                     LGDTKTLLPDTQALLEATKQLVKEGFTVLPYTNDDPILAKRLEESGAAAVMPLAAPIG
                     SGRGVQNPLNIQFILETVTHVPVIVDAGVGTASDAAVAMELGVDGVLMNTAIADAQDP
                     VLMAKAMQQAVSAGRHAFLAGRMEKKIYAAPSSPTQGVVAPLTEKTSS"
     gene            4442..4972
                     /locus_tag="P5079_09445"
     CDS             4442..4972
                     /locus_tag="P5079_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30243.1"
                     /translation="MTDDLSMRSAVLRPVVLIAAALLWAAPGFAKPRSGVVSPDFDGK
                     PIGARYIGLGEIGAVATGMPESPVWNPASLNDLTMPMMSVDFDVARQSRLEDDVLLSD
                     VPLRGRKLTYLGFAATDGAFFYRPLASYKTRTVDNADPLNNFTETALSISQFGFSASC
                     TGPAAVFTVCMSAGIQ"
     gene            complement(4897..>5193)
                     /locus_tag="P5079_09450"
     CDS             complement(4897..>5193)
                     /locus_tag="P5079_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30244.1"
                     /translation="GGFYTTLMVGIDPRLKAGSCFFGAGGLQLGNNWVPYPAKDAAWA
                     ERWAKTLDPAWRLAQVKTPMNWCTGTNDFAYWMPALMHTVKTAAGPVQLALKPN"
BASE COUNT          934 a         1891 c         1470 g          898 t
ORIGIN      
        1 ccttccggcc cccgacccgg aaccgccggc ccacgccgcc cctctcctgg gcctcctccc
       61 cggcagccgc cgcggggaaa tccgccgcca tctgggcctc ttcctgcaaa cggccgaccg
      121 gctgacccag gacctcccga atctccgatg cgctcttttc gccgcccctt ccatcccgga
      181 cgacttttac aaccctcttc tggcccggca tccccatcgg accttcccct tggaaatcgt
      241 ccgggaagaa cattatgcgc aacgcgccca actggacctg gccctcacct gttccggcac
      301 cgccacgttg gaaaacgccc tgttgggcat ccccatgctg gtggtctata aaatgtcctg
      361 gatcacctac ggactggcgc gggccctcat ccgcgtggag cacatcgcca tggccaacat
      421 cctggcgggg aaaaagctcg tcccggaact catccaagac caggcctcgc cggccctcct
      481 ggcccaggaa gccctccggc tcctgaaaga tccccaggaa cgcctccgcg tgcggcggga
      541 actcctctct cttcgccaac aactgggcgg acccggcgcc gccgaccgcg ccgccgccga
      601 aatcatccag gccctgggat aactccatgc aaacgcccgt cgtccgccgc ctcatccatt
      661 atttccgggg ccacctcccc atgctgaccg tggcgctggt ggccatggcg gccgtggccg
      721 ccctcaccgc cgccgccatg tggatattga aacaggtggt ggacaaggcc ctcaccgccg
      781 gcgacctcac cgctctgcag gaaatcgtcc ttctcgtcgt ggtccttttc gccatgaaag
      841 ccgtgctctc ctacgtgcac gactacctta ccgcccacgt cggccaggcc atcgtccgcc
      901 aattgaggga cgacgtctac gcccacctgc acaccctctc catggatttc ttcaccttga
      961 acgattcggc gcgcctcatc tcgcgcctca ccaacgacgc ccaattggtc cagaacgccc
     1021 tcaccaagat ccccgtgatg atcgtccgcg acgggctcac catcctctgc ctcaccggct
     1081 tcctccttta cctgcactgg aaattcgcgc tcctggcgtt caccatcctg ccggtgtcgg
     1141 gctacctcat cgcgcgcttc ggcaagagcc tgcgcaagac cgcccggctg ggccaggcca
     1201 agatgtccga cctctatctg cagatccaag agaccatcac cggcgcgccg gtggtcaagg
     1261 cctacctgcg ggaaaactat gagagggacc ggttccgtcg ggaaaaccac gcctatttca
     1321 ccatcaccat gaaaaacgcc cgggtggaat cgctctccag tccggtgatg gaattcttgg
     1381 gcgccatcgg catggcggcc atcctctggc tgggcgggct ggacgtcatc aacggcgtct
     1441 ggaccacggg cgcgttcttc gccttcgtcg gcagcgcgct gtcgctctac cagccggtga
     1501 aaaatttctc gcgcagcaac gccaccctgc aactggccct ggccggcgcc gaacggatct
     1561 tcgagatact cgacacccag cccaccgtca tggaaaaacc gggggcgccg cccctgccgc
     1621 cctttaccac cgccttgaaa ttcgaaaacg tcaatttcca ctaccggccc gaccggccgg
     1681 tcttgaaaaa catcaacgtc acggtctcca aaggggaaat cgtggcgctg gtcggcccgt
     1741 cgggctccgg caagaccacc atggccgccc tcctcctgcg cttctacgac ccggtgagcg
     1801 ggcgcgtggc cttcgacggc cacgacctcc gcgacgtctc cctggccggg ctgcgcggac
     1861 agatcggact ggtcacccag gaaaccttgc tcttcaacga caccgtccgc cacaacatcg
     1921 gctacgccaa ggccaacgcc accgaacagg aactcatcgc cgccgcccgg gccgccaacg
     1981 cctgggagtt catcgagaaa cttccccagg gcctcgacac tctggtgggc gaacgaggcg
     2041 tgctcctctc cggcggacag aaacaacgcc tggccatcgc ccgcgccatc ctcaaaaacc
     2101 cgcccatcct ggtcctggac gaagccacca gcgccctcga cgccgagtcg gaacggctcg
     2161 tgcaggaggc ggtcgagcag ctcatgaaga accgcaccgt catcgtcatc gcgcaccggc
     2221 tcgccaccat caagaacgcc gaccgcatcg tggtgctgga acacggccgc gtggccgaaa
     2281 tgggcacaca caccgaactg ctcagcaaaa aaagcctcta ccaccggctt tacgagctcc
     2341 aactgttgca ggaatgaccg gcccatgccc ctcccgctga ccttttccct catctcggtt
     2401 ttcgccacgc tgggcggcgg ctggctcctt ctcggcagga aagaatggac gctgaaatac
     2461 ctctggcggt tcctcgcctt cggcagcggc gtcatcttcg gcatcacctt cctgcacctc
     2521 atccccgagt cgtggcggct ggactcccgc tggggcgcgg ggtccctcct cgtcgccttc
     2581 gtgacgttct acgctctgga ggagttcgtg gtggcgcacc acgcctgcgg cgaactcacc
     2641 gaggattgtc acgaacacaa gttcggctac ggcgcgctgg tcgcgctgtt cctgcacagc
     2701 ctctccgacg ggctggccat ggcgttctcg tttctgtcct ccacggccct gggggtgggg
     2761 gtctccaccg ccgtcatcgt gcacaaattt tccgacggca tgacgctttc cagcctcttc
     2821 ctcaaccagg gattctcccg aaagaaaatc tgggccctga cctccgtcct ctccatcgcc
     2881 acgccgctgg gactgatgat cggtctgtcc gccaccgggc tcatcacgcc caccgtgttg
     2941 gccgttctcc tggggctggc ggcgggaagt ttcctctatg tggccatggc cgacatcctg
     3001 ccgcgcctcc accacaacaa ggacatccat tcctggatcc tcttcctggt gggcatcggg
     3061 ctcagcgccc tctacccgca ctgacattca catgccggcg tcattgacca tccaggtgaa
     3121 cggggaaaaa cgacaggtgc cggacggcgc caccgtccag tcccttctgg aatcgctggg
     3181 gatgggcgcc ggccgcgtgg cctgcgaact caacttgaac atcgtcaaac gggcggattt
     3241 tccttcgacg cgactgaagg agggggattc cgtcgaactc gtccagatga ttggcggcgg
     3301 atgaccgagg cgagggagcc gaggaaatgg ttgtaaggcg cgagcctgaa aaccatgacc
     3361 gaggcgaggg agccgaggat gccgctcggc atggtccctg ttctgcttgg ccgcccggcc
     3421 aggcccctgt ttgtggaaag gggtcgggag ggaggaaacc cctcgcagcg ctttggggcc
     3481 tacggcggtt ccggaggaac cgaaaccccg caggtggttg taaggcgcga gcttgaaaac
     3541 catgaccttc ggcaccggcg ggacgccgcg gcgcccgcgg aaagcgcaaa cgcgccgaga
     3601 atagatttat gtccgttact caagtttgct agaatggagt tcttatgaac gacaaaccgc
     3661 tcgtcatcgc cgggaaagaa tacacgtcga gactcatcgt cggcacggga aagtacaaga
     3721 cccttccgct gatgaaagag gcgctgacgg cttccggcgc ggagatggtc accgtggcca
     3781 tccgccgcgt gaacctgcag gacaaatcgc ccgaatcgtt ctggaaccac atccccgaag
     3841 gcatgaccat cctccccaac accgccggtt gttacaccgc gggcgacgcc gtccgcatcg
     3901 cccgcctggc ccgggaagtc cttcaaacgc ctctcatcaa actggaggtg ctgggtgaca
     3961 ccaagaccct cctgcccgac acccaggcgc tcctcgaagc caccaaacaa ttggtgaaag
     4021 agggtttcac ggtgctcccc tacaccaacg acgaccccat cctcgccaaa cggctggagg
     4081 aatccggggc ggcggcggtg atgccgctgg ccgcacccat cgggtccggc cgcggcgtgc
     4141 agaacccgct caacatccaa ttcatactgg aaaccgtcac gcacgtcccc gtcatcgtgg
     4201 acgccggcgt ggggaccgcc tccgacgccg ccgtggccat ggaactcggc gtggacggcg
     4261 tgctgatgaa caccgccatc gccgacgccc aggacccggt gctcatggcc aaagccatgc
     4321 aacaggccgt gtccgcggga cgccacgcct ttctggcggg gcgcatggaa aagaaaatct
     4381 acgccgcccc ctcttcgccc acgcagggcg tggtcgcgcc tctcaccgaa aagacgtcgt
     4441 cttgaccgac gacctttcga tgcgctccgc cgttcttcgc cccgtcgtcc tcatcgccgc
     4501 cgcgctcctc tgggccgccc ccggttttgc caaaccccgg tcgggcgtcg tctcgcccga
     4561 cttcgacgga aaacccatcg gcgcccgcta catcggcctc ggggagatcg gcgcggtggc
     4621 caccggcatg ccggaatccc ccgtctggaa ccccgcctcc ctgaacgacc tgaccatgcc
     4681 catgatgtcg gtggatttcg acgtggcgcg gcagagccgg ctggaggacg acgtcctcct
     4741 gtccgacgtg cccctgcgcg gacgcaaatt gacctacctg gggttcgccg ccacggacgg
     4801 cgcgttcttc taccgtcccc tcgccagcta caagacccgc accgtcgaca acgccgaccc
     4861 gctcaacaac ttcaccgaga cggccctctc catcagtcag ttcggcttca gcgccagctg
     4921 caccgggccg gccgcggtct tcacggtgtg catgagcgcg ggcatccagt aggcgaagtc
     4981 attggtgccg gtgcaccagt tcatcggcgt cttcacctgc gccagccgcc aggcggggtc
     5041 cagcgtctta gcccagcgtt ccgcccaggc cgcatctttt gccgggtagg gcacccagtt
     5101 gttgcccagt tgcaggccgc cggcgccgaa gaagcaggag ccggctttca agcgcgggtc
     5161 gatgcccacc atcagcgtgg tgtagaagcc gcc
//
LOCUS       DAOTFD010000188         5146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11690_length_5146_cov_3.766254, whole genome shotgun sequence.
ACCESSION   DAOTFD010000188 DAOTFD010000000
VERSION     DAOTFD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11690_length_5146_cov_3.766254"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5146
                     /locus_tag="P5079_09455"
     CDS             <1..>5146
                     /locus_tag="P5079_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY30245.1"
                     /translation="GGGTFGAVTVSTQTMNQSRGVAWGDFDKDGDLDLLAGFYNGALS
                     YVTVFRYDGANTFVKSWWHQGTSEYNTSVSWMDYDNDGWLDIVVAQDMANQVRVYRNK
                     RDGSFAEVSMGVIGVLGINGNSVQAGDYDNDGDLDLLFGNYNNIQGPIIRRNDGDAAN
                     PRYVTSWNSAVAEPVRQAQWGDIDNDGDLDFALAKYNVPIEVYQNQGGSFAMIWQSTI
                     SEPTYSVAWGDYDKDGYMDLAVGNYGAANRIYRNAGNRTFLPVWQATATYNTRYVAWA
                     DVDNDGDLDLSVASYGGSKIVYKSNVAYYEGTNNKPFAPSSGFFSTYDGGSNRVRMRW
                     DDGADTETPAKGLYYNVQASSQTPVQDWNANLLPWRQASPFFGAYPHGFAVAVSTQPG
                     MDWKPFVEGTSVYWRVQTVDAGFSTSPWSSEYSTYVPNVPPTAPDLVSPLDGFTTNVM
                     TINFDWTDSTDTISGVAGYEFQHSTDAVFDVNDGTETLSFSVFQATDVPAETVYWRVR
                     AFDKAGNYGDWSGYRMCTRTPIEFRWVGPDFGYASSSVNWSPQGIPDNNDSVGFYPSS
                     NTYCYWDLWDVELSSLTIYSGYTSTVNVMTAGNTITLKEDFSQASGGMYTASAGFTLR
                     VGGDFLVSGGTVTTGPSDGGEVRFIDSATHNYNAPGVSLGNLFVSSGTVTLQSDLYLK
                     GNLNISSGTLSAGNHLIRVGHNWNQDATGGFDAGTSTVQFRRTDNAFSHSINLPAGTS
                     FYNLDVRTYSTVYVYMSSPVVVLGDLSISSGNLSSNGYTTTLHGNVYRSSNTYWGVST
                     SSVVFAGAADQSVTTDGGGTFDFYRVEVNKSPGSRVTLSAPASFNGGLTLSGGIFDSG
                     SSSHTLWGSIFSTQGNMDNQDSTWTLKGSNYQVLPSTVAWGTLVIDKYDAAIPATVTF
                     TMDGATPTINNLYVPYNSGGAATGLDISTNQAVSYVRIAGDLIVENTTHFRIEGSTIE
                     FTGPGESVIKSNGYGQVKAGNIIIDKTGGWSLWGSLGIDGNLVIENVNGFYLGDASCV
                     TTLSGSFLKNGGGVFSLAGGTIAFSGDTTHYLKLAASDALSRVDVNGGVLALMSTATV
                     NDDFNWNGGNIDLYSAATPDADLYVGGDWMQGCSAAGFYAGDGRTILNGTKEQILTGS
                     CGPAYMGNLVIRSTVTSNGAMIAEDFSLELGTYNATTFWYNQFVRGDFSQSPGTYFVS
                     GNSTFYFNGDAPQVIALQPGSSFYNFVSSGALVSAGSDLNIVGEFSLASGTFDTNGAT
                     VTLSRHMNYTNGAFDGTGLWVFNGANLVDQSVYVSTFDNVRVDKADGWALYFWNGLDA
                     DGDVLFSTNSIVDFDMHASTFRGDFIAYGTMTDLPGARFVGGVPAAIHTSSSTSAFQD
                     LTISKDSGVSVTATSPLWVAGSFYVQNGVFNAGDGLFAIKNDFIGSGGTFNPNNGTVR
                     LEGYETGGNTVVQLPAGSSFNFLIVEKEVMSTMSHTVYFNSAIDAGYLYVNNTMPTGF
                     LTLSPNDYAHTLSGDFYLGGSGSDVQMTSGDFTFDGIGTQHVGRLNGTTTLQMSTFTV
                     AGSSVAAISPIRVDNVWELSSGEFVPGTFTHLVNGAFYAASGVGFDPSTGTFQFMDSA
                     YVGALRYVQLGDAFQHFSNFEVNAASVVAQSDLNIRGTLDMEAGGGDPDSRFNPNGHQ
                     VNLGGDLKAYDGNSLVPSGTFYFDGGTVQAISNSMGADATLNHLVID"
BASE COUNT         1016 a         1453 c         1554 g         1123 t
ORIGIN      
        1 gggggaggga cgttcggcgc cgtcacggtc tccactcaga cgatgaacca gtcccgcggc
       61 gtggcctggg gggacttcga caaggacggc gacttggacc tgctggccgg tttttacaac
      121 ggggcgctta gttacgtgac ggttttccgg tatgacggag ccaatacgtt tgtgaaatca
      181 tggtggcatc aaggcaccag cgaatacaac acctccgttt cgtggatgga ttatgacaac
      241 gacgggtggc tggatatcgt tgttgcccag gacatggcca accaagtccg ggtataccga
      301 aacaagaggg acgggtcttt cgcggaagtg tcgatggggg tcatcggggt tttggggatc
      361 aatggaaact ccgtgcaggc cggcgactat gacaacgacg gcgacctgga cctcctcttt
      421 ggcaattaca acaacatcca ggggccgatc atcagacgga acgacggtga tgcggccaac
      481 ccgcggtacg tgacctcatg gaattccgcc gttgccgaac ccgtccggca ggcccagtgg
      541 ggcgacatcg acaacgacgg agacctggat ttcgccttgg ccaaatacaa tgtgccgatc
      601 gaggtatatc agaaccaggg cggctctttt gcgatgattt ggcagtccac catttcggag
      661 ccgacctact ccgtggcgtg gggcgattac gacaaggacg gctatatgga cctggccgtc
      721 ggcaattacg gcgccgcgaa ccggatttac aggaacgcgg gaaacagaac attccttcct
      781 gtctggcagg ccacagccac ctacaacacg cgttatgtgg cctgggcgga cgtggacaac
      841 gacggcgatt tggatttgtc ggtggccagt tatggaggca gcaagatcgt ctataagtcc
      901 aacgtggctt attatgaggg gacgaacaat aaacctttcg ccccttcctc cggctttttt
      961 tccacttacg atggtggttc caaccgcgtg cggatgcgct gggacgacgg ggccgatacc
     1021 gagacgccgg cgaaaggtct ctactacaac gtgcaggcgt cgtcgcagac gcccgtgcag
     1081 gattggaacg ccaatctgtt gccttggcgc caagcctcgc cgtttttcgg cgcttatccg
     1141 cacgggtttg ccgtcgccgt ttccacgcag ccggggatgg attggaaacc ttttgtcgaa
     1201 ggaacctcgg tgtattggcg cgttcaaacg gtggacgcgg ggttcagcac cagcccctgg
     1261 tcgtcggaat actccaccta tgtccccaac gtgcctccca ccgcgcccga tctggtcagc
     1321 ccgctggatg ggttcaccac caacgtcatg accatcaatt tcgactggac cgattccacc
     1381 gacaccatct cgggggtggc gggctacgag tttcagcatt ccacggatgc ggttttcgat
     1441 gtgaatgatg ggaccgaaac cctgtcgttt tcggtgtttc aggccaccga cgttcccgcg
     1501 gagaccgttt attggcgtgt gcgcgccttc gacaaggccg gcaattacgg cgactggagc
     1561 ggttatcgga tgtgcacgcg cacgcccatc gaattccgat gggtcggccc ggatttcggc
     1621 tatgccagca gcagcgtcaa ctggagcccc caggggattc ccgacaataa cgattccgtc
     1681 ggtttctatc cttccagcaa cacctattgc tattgggacc tgtgggacgt ggagttgtcg
     1741 tcacttacca tttattcggg atacaccagc accgtcaacg tgatgaccgc cggaaacacc
     1801 atcacgctga aggaggattt cagccaggcg tccggaggga tgtataccgc ttcggcgggt
     1861 ttcacgctcc gcgtgggggg cgacttcctt gtttccggcg gaacagtgac caccggtccg
     1921 tcggacggcg gggaggtccg gttcatcgat tcggcgacgc acaattacaa cgcgccgggc
     1981 gtctccctcg gtaatttgtt cgtcagttcc ggcaccgtaa cgctccaaag cgacctttat
     2041 ttgaagggca acctgaacat cagttcgggg accttgagcg cggggaacca tctcatccgg
     2101 gtggggcaca actggaacca ggatgcgacg ggggggtttg acgccgggac cagcaccgtg
     2161 cagttccggc gcacggataa cgcgttcagc cactcgataa atcttcccgc gggaacgtct
     2221 ttttacaatt tggatgtgcg gacgtactcc actgtctatg tgtatatgtc ctcccccgtc
     2281 gtcgttttgg gggatttgtc catcagttcg ggtaatttga gttcgaatgg ctacaccacg
     2341 acgctgcatg ggaatgtcta tcgctcgtcc aacacgtatt ggggcgtttc cacgagttct
     2401 gtcgttttcg ccggtgccgc cgaccagtcg gttacgacag acggcggagg cacgttcgac
     2461 ttctatcggg tggaagtcaa taaatcgccc ggatcccggg tgaccttgtc ggctccggcg
     2521 tcgttcaatg gagggttgac gttgagcggc ggcattttcg attccggaag ttcgagccat
     2581 acgttgtggg gaagtatttt ttccacgcag gggaacatgg acaaccagga ctccacctgg
     2641 accctgaagg ggagcaatta tcaggtcttg ccgtcgacgg tcgcctgggg gacactggtc
     2701 atcgataaat acgatgccgc cattccggcg acggtcacgt ttaccatgga cggcgccacc
     2761 cccaccatca ataatctcta tgtcccctac aacagcgggg gagcggccac gggtttggat
     2821 atttccacga atcaggccgt aagttacgtc cgtattgccg gcgatttaat tgttgaaaac
     2881 accacccatt tccgcatcga ggggtccacc atcgaattca cgggccccgg cgaaagcgtg
     2941 atcaaaagca atggttatgg gcaggtcaag gcgggcaata tcattatcga caagaccggc
     3001 ggctggtctc tttggggctc gttggggatc gacgggaacc tcgtcattga aaatgtcaac
     3061 ggtttctacc tgggagacgc ctcatgcgtg accacccttt cgggaagttt tctgaagaac
     3121 ggaggggggg tgtttagtct cgccggcgga acgatcgctt tttcgggcga cacgacccac
     3181 tacctcaaat tggccgcctc cgacgcgttg tcgcgggtgg acgtgaacgg cggcgtgctg
     3241 gcgctgatgt ccaccgcgac ggtgaacgac gatttcaatt ggaacggcgg caacatcgac
     3301 ctttattccg ccgccacccc ggacgcggat ctgtatgtgg gcggcgactg gatgcaaggg
     3361 tgttcggccg ccgggttcta tgcgggagac gggcggacga tcctgaacgg gaccaaggaa
     3421 cagatattga cgggcagctg cggtcccgcc tatatgggga acctggtgat ccgttcgacg
     3481 gtgacttcca acggggccat gatcgcggaa gatttcagtc tcgaattggg gacctacaac
     3541 gccacgacct tctggtataa ccagttcgtc cggggggact tttcacagtc gccgggcacc
     3601 tacttcgtct ccggcaacag cacgttttat ttcaacgggg atgctccgca ggtcatcgcg
     3661 cttcagccgg gttcatcttt ttataatttc gtgtcgtccg gggccctggt ctcggccggg
     3721 tccgatttga atattgtcgg cgaattttcg cttgcgtccg ggaccttcga cacgaacggc
     3781 gccacggtga cgttgagccg ccatatgaac tacaccaacg gcgcgttcga cggcacgggg
     3841 ctttgggtgt tcaacggggc gaatttggtc gatcagtccg tctatgtcag caccttcgac
     3901 aatgtccgcg tggacaaggc cgacggctgg gccctctatt tctggaacgg attggacgcg
     3961 gacggggacg tcctcttcag cacgaacagc atcgtcgatt tcgacatgca tgcgagcact
     4021 ttccggggag acttcatcgc ctacggaacc atgaccgacc ttccgggggc acggttcgtc
     4081 ggaggcgtgc ccgcggccat ccatacctcc agcagcacga gcgcgttcca ggacctgacc
     4141 atcagcaagg attccggcgt ttcagtcacg gccacatcgc ccctctgggt tgccggcagc
     4201 ttttatgttc agaacggggt gttcaacgcc ggggacgggc tgttcgccat caaaaacgat
     4261 ttcatcgggt ccggcggcac gtttaatccg aacaacggga ccgtccggct tgaaggatac
     4321 gagaccggcg ggaacaccgt ggtgcaattg ccggccgggt cttcttttaa tttcttgatc
     4381 gtcgaaaaag aagtgatgtc gacgatgtcg catacggtgt atttcaacag cgccatcgac
     4441 gccggttatc tctatgtcaa caacaccatg cccaccggtt ttctgaccct ctcgcccaac
     4501 gattacgcgc acaccctcag cggcgatttc tatctgggcg ggtccgggtc ggacgtccag
     4561 atgacctccg gggatttcac gttcgatggg atcggcaccc agcacgtggg gcgattgaac
     4621 ggcacgacga cgcttcagat gtccaccttc acggtggcgg ggtcaagtgt ggctgcgata
     4681 tcgccgatcc gggtcgataa tgtctgggaa cttagttcgg gggagttcgt ccccggcacg
     4741 ttcactcatc tggtcaacgg ggcgttctac gcggcctccg gggtggggtt cgatccgtca
     4801 accgggacgt ttcagtttat ggattccgcg tatgtggggg cccttcgtta cgttcaattg
     4861 ggagatgcct tccagcactt cagtaatttt gaggtgaacg ccgcgagcgt cgtggctcag
     4921 agtgacttga acattcgcgg cacactcgat atggaagccg gcggcggtga tcccgattcg
     4981 cggttcaacc ccaacggcca tcaggtgaac ttggggggag atcttaaggc ctacgatggg
     5041 aactccctgg tcccctccgg caccttctat tttgacggcg ggaccgtcca agccatctcc
     5101 aacagcatgg gagcggacgc caccctgaac cacctggtca ttgacg
//
LOCUS       DAOTFD010000189         5110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11801_length_5110_cov_4.910188, whole genome shotgun sequence.
ACCESSION   DAOTFD010000189 DAOTFD010000000
VERSION     DAOTFD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11801_length_5110_cov_4.910188"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="P5079_09460"
     CDS             <1..643
                     /locus_tag="P5079_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025824.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRY30246.1"
                     /translation="KGFPSLPAPSTLSGIVVMGGPMGVYDQSRFPFLKKELGYIRRCL
                     ERHLPALGICLGAQMIARALGARVYPSRQKEIGWYPVRLTAAGRRDPFFRESSGGKET
                     GRRIVFQWHGDTFDLPRRAELLATASRCRHQAFRCGGNVYALQFHLEVTPEMVRSWMK
                     APGAREELRLLGPRAAEDILRKMPRCRPQMHRTATAAFRAFSEIVKKRRETHE"
     gene            636..2588
                     /locus_tag="P5079_09465"
     CDS             636..2588
                     /locus_tag="P5079_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY30247.1"
                     /translation="MSRKEFRAALVVMPFQSVSTSNLGAAVLKSVLRRAGFSCDAHHF
                     NARFAEKTGLEFYERLSPLRVFPETIFAHALFGKGGTGEIDYPRRFISSLSSVDTFSR
                     ETLAEIGGAPKDFYRLLDRTVPETLEECFRSVDWSAYGLVGFSSVFNSHVASLALARK
                     IKEKFPRVKIVFGGSNVEGEMGWETLRAFEWVDYVVNGEGEAALVRLARALADGKTVV
                     DVPGVSFREGGRLCIDRRRAPLADLSRVPRPDYADYFSALRRTGLAGRLKPTLVFEGA
                     RGCWWGEKSHCLFCGHNGLAMKFRRKTPTRVLRELMHFMREYGVEQFQASENTVDMEF
                     PRKFFPRLAEELKRRRLEPTFFYEIRATLSKEQLAALAAAGARYLQPGIESFDTDLLR
                     KMRKGVTAIRNIQLLKHCRELDILPIYMMLYGFPMEKAGNYRTVMRLLPRLTHLTPPR
                     ALNRVRLDRFSPLQEDPRRWGFAAVRPNKMYDWVYPKSRVDIGKIAYYFQGKPRRRPP
                     VLDTYFEDIEKFVEKWKALYRRSFLVHRSVWNAVIVYDHRLGNFESGGLEFRVHRYEG
                     RWPARVLEFCDSARRWEELREHLLKAGRRENEGELRRLLSRMVRDGILLKEGGCYLSL
                     SLPYQTQGIELKDTILRLEKLRDLFE"
     gene            3048..3851
                     /locus_tag="P5079_09470"
     CDS             3048..3851
                     /locus_tag="P5079_09470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRY30248.1"
                     /translation="MLPLISVVVPVYNREKYVIQALKSVERQGYAPLEVLVIDDGSVD
                     RSLRRIQGRSWKFPLRILKLRRSAGPSVARRVGIKNALGTWIAFLDADDRWHPDKLSE
                     LMSRRTASTRFLFSDASLVSGAGKVLARKAMKKLQTRAYRKKIRSLWGVPFFPLTSTV
                     LVERRFLERAGSFDPQFRHVAEDVDVWFRLLRKLRPGGVFCSPRPLTCYRRHRGQTSY
                     FVRSPGVTTHQLWLQKRPGGGAEKEEKSLDAAMWWLKNGRDWLALTRDL"
     gene            complement(3858..4640)
                     /locus_tag="P5079_09475"
     CDS             complement(3858..4640)
                     /locus_tag="P5079_09475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30249.1"
                     /translation="MPKNSPRVSVIIPTRNSLRTLREALKSVFRQTYPFYEIVVVDDA
                     SRDGTARALAPMAQAGRLRYIKLRRPVGAAEARNIGIRATKTPFVAFLDADDLWRPEK
                     LAVQMRALEKTGALFCYSRYTVVGPAGRTLAHPTWRSFGGFPRIGLPLYPRTSTCVLS
                     RQIFRRVGVFDPIFKLRHDETDFFYRVRQKLGKRAFLYLSRPLVKYRFSPDRHSAYWK
                     TGARAAPFQIQRRFDLFHFFEKHPRAGHIFARSAYERAWRKG"
     gene            4735..>5110
                     /locus_tag="P5079_09480"
     CDS             4735..>5110
                     /locus_tag="P5079_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY30250.1"
                     /translation="MANRRAFDIVRRGGVVVLWLALAMVFPGRLLAAEAGSKKIKVFL
                     VGVDGGTWGILNGLVEAGAAPNFRRLMEAGTYGVLKSTEPIYSPVLWTSIATGLAPEK
                     HGITHFFKDPAHPVRQVNSSDRK"
BASE COUNT          979 a         1400 c         1652 g         1079 t
ORIGIN      
        1 gaaagggttt ccctctctcc cggccccgtc gaccctgagc gggatcgtcg tgatgggcgg
       61 tcccatgggg gtctacgacc agtcccgctt tcccttcctt aaaaaagaac tcggatacat
      121 ccggaggtgc ctggagaggc atttgccggc gctcggcatc tgtctggggg cccagatgat
      181 cgcccgtgcg ctgggggccc gggtctatcc cagtcggcaa aaagagatcg gttggtatcc
      241 cgtgcgactg accgcggccg gccgtcggga cccttttttt cgggaatcgt ccggcggaaa
      301 agaaacgggg cgtcggatcg tttttcagtg gcacggcgac accttcgatc ttccgcgccg
      361 ggcggaactt ctggccaccg cttcccggtg ccgtcatcag gcttttcgtt gcggcgggaa
      421 cgtttacgct ttgcagttcc atctggaggt cacgccggag atggtccgtt cctggatgaa
      481 agcgcccggc gcacgggagg agctccgtct tctgggtccc cgtgccgccg aggacatcct
      541 ccggaagatg ccccgctgtc ggccgcagat gcacaggacg gcgacggcgg cgttccgcgc
      601 gttttcggag atcgtgaaaa aacggcgcga gacccatgag tagaaaagaa ttccgcgcgg
      661 ccctcgtcgt catgccgttc caatcggtct ccacgagcaa tctcggggcg gccgtcctca
      721 aaagcgttct ccgtcgcgcc gggttctcct gcgatgccca tcatttcaac gcccgctttg
      781 ccgaaaaaac gggactcgaa ttctacgaga ggctttcgcc gctccgcgtg ttcccggaga
      841 cgatattcgc ccacgcgttg ttcgggaaag ggggaaccgg cgagatcgat tatccccgcc
      901 ggttcatttc ctctttgtcg tcggtggata ccttcagccg ggaaacgctg gcggaaatcg
      961 ggggcgctcc gaaagatttt taccggctgc tggaccggac cgttccggaa accctcgagg
     1021 agtgttttcg gtcggtggat tggagcgcct atggcctcgt cggtttttca agcgttttca
     1081 acagccatgt ggcgtccctc gcgttggccc gaaaaataaa ggagaaattc ccacgcgtca
     1141 aaatcgtttt cggtggatcg aacgtggagg gcgagatggg gtgggaaact ctccgggcct
     1201 tcgagtgggt cgactacgtt gtgaacgggg agggggaggc ggcgctggtt cgactggcgc
     1261 gcgcgttggc cgacgggaaa acggtcgtcg acgtgccggg ggtctcgttt cgcgagggag
     1321 gccgtctttg cattgatcgc cggcgcgcgc cgttggccga tctttcccgg gtcccccggc
     1381 ccgactacgc ggactacttt tcggctctcc ggcggaccgg gctggcgggc cgtctcaagc
     1441 ccacgctcgt tttcgagggg gcgcggggat gctggtgggg cgaaaagagc cactgtcttt
     1501 tttgcgggca taacgggctg gcgatgaaat tccgtcgaaa aacgcccacg cgggtgcttc
     1561 gcgaattgat gcatttcatg cgggaatacg gtgtcgaaca gttccaggcg tcggaaaaca
     1621 cggtggatat ggaatttccc cggaagtttt ttccgcggtt ggcggaggaa ctgaaaaggc
     1681 ggagattgga accgacgttc ttctatgaaa tccgcgccac cctctccaaa gagcaattgg
     1741 ccgccctggc cgcggcgggg gcccgctacc tgcagccggg gatcgaaagt ttcgacacgg
     1801 atttgttgcg gaagatgcgc aagggggtga cggccatccg caacatccag ctcctcaaac
     1861 actgccgtga attggacatc ctccccatct acatgatgct ctacggtttt ccgatggaaa
     1921 aggccgggaa ttaccggacg gtgatgcggc ttcttccgcg cctgacgcat ctcacgccgc
     1981 cgcgcgccct caaccgcgtc cgcctcgacc ggttcagccc gcttcaggag gatccccggc
     2041 gctgggggtt cgccgccgtc cgtccgaaca agatgtacga ttgggtctac ccaaaatcgc
     2101 gggtggacat cggaaagatc gcctactact ttcagggaaa accgcggcgg cggccgcccg
     2161 tcctcgatac gtattttgag gatatcgaaa aattcgtgga aaaatggaaa gcgctgtatc
     2221 ggcgaagttt tttggttcat cggtccgttt ggaacgccgt gatcgtttat gaccatcgcc
     2281 tggggaactt cgagtcgggc gggttggaat tccgcgtcca tcggtacgaa ggacgttggc
     2341 cggcccgggt gctggaattc tgcgattccg cccgccgctg ggaagaactg agggagcacc
     2401 tcctcaaggc ggggcggcgg gaaaacgaag gggaactccg gcggcttttg tcgaggatgg
     2461 tgcgggacgg gattttgttg aaggaaggcg gatgctatct ttcgttatcg ctgccctatc
     2521 agacgcaagg gattgaatta aaggacacga ttttacgatt ggaaaaacta agggatttgt
     2581 tcgaatgagg gataaaaaat cgatattttt aggcgtttga gagatatttc cgaagggggc
     2641 cccctcggtc aaagtgttgc atatcgagtg ttcaacaggg ggctggaact cggcggttcg
     2701 ttgaagacag ggtaaaatgg ttctcccgtc gcgttttgga aaagaaggcg ttaagttgct
     2761 tttcgaattt ggtttatagt atactggaat atccgcctag taaacgtaag gagatttgtc
     2821 atgcgtcgta aagtcagatc ctccaaaccc gccggaaaga agacctacaa gaaaccttcc
     2881 atcacaaagc atggacgatt gcacgtctac gcctccccgc ctttctgaaa ggaagtcttt
     2941 ttgagacggc gatgtctctt cgtccgccgg tcacgtaatt ttttccctcc cgcgtttttt
     3001 gaaatcgtcc gccgttccct accggttccc ctcccgtctt ttcctcaatg cttccgctca
     3061 taagtgtcgt tgtcccggtc tataaccggg aaaaatacgt cattcaggcg ttgaagagcg
     3121 ttgagagaca gggatacgct ccgttggagg tgctggtcat cgacgacggc tccgtggacc
     3181 gttctctgcg gcggatccag gggcgttcct ggaagttccc gctgcggata ttgaagctcc
     3241 gtcgctccgc cgggccttcg gtcgcccggc gggtgggaat aaaaaacgcc ctcgggacgt
     3301 ggatcgcttt cctagacgcc gacgaccggt ggcatccgga caaactctcc gaattgatgt
     3361 cgcggcgaac cgcctccacg cgtttcttgt tttcggatgc gagcctcgtc tccggcgcgg
     3421 ggaaagttct ggcgcggaag gccatgaaga agctgcagac gcgggcttat cgaaaaaaga
     3481 tccgctcgtt gtggggcgtt ccgttttttc cgttgacgtc gacggtcctg gtggaaaggc
     3541 gctttttgga gcgcgcgggt tcgttcgacc cgcaattccg gcatgtcgcg gaggacgtcg
     3601 atgtgtggtt ccgactcttg cggaagctca ggccgggcgg cgtgttttgt tccccgcggc
     3661 cgttgacgtg ctaccgccgg catcgagggc agacgtccta tttcgtgcgg tcccccggcg
     3721 tcaccacgca ccaactctgg ctgcaaaaga ggccgggcgg cggcgcggaa aaagaggaaa
     3781 aaagtctgga cgccgccatg tggtggctga agaacggcag ggactggttg gcgctgacga
     3841 gggacttatg aagcggatca gcctttgcgc cacgcgcgtt cataggcgga gcgcgcaaag
     3901 atgtggccgg cccggggatg cttttcgaaa aagtggaaca gatcaaaacg gcgctggatt
     3961 tgaaagggag ccgcccgggc ccccgttttc caataggcgc tgtggcggtc cggtgaaaaa
     4021 cgatatttga cgaggggccg agaaaggtag aggaacgccc gcttccccag tttttgccgc
     4081 acgcggtaaa aaaaatcggt ttcgtcgtgc cgcagtttga aaatcgggtc gaaaacgccg
     4141 acccggcgga aaatttggcg cgagaggacg caggtggaag tcctggggta aagagggagc
     4201 ccgatgcgcg ggaatccgcc gaaactccgc caggtgggat gcgcgagtgt ccggcccgcg
     4261 gggccgacga cggtgtagcg ggaataacag aagagcgccc cggttttctc cagggcgcgc
     4321 atttgaaccg cgagtttttc cggcctccag aggtcgtcgg cgtcgaggaa ggcgacgaag
     4381 ggcgttttcg tggcgcggat tccgatgttg cgcgcctcgg cggcgcccac gggccggcgc
     4441 agtttgatgt accggagccg tccggcttgg gccatcggag ccagggcccg ggcggtcccg
     4501 tcccgggagg cgtcgtccac cacgacgatc tcgtaaaaag gatacgtttg acggaagaca
     4561 cttttcagcg cttccctcaa cgtgcgaagg ctgtttcggg tggggatgat gacactgacg
     4621 cggggagaat ttttcggcat gagcgacgat ccgtttgaat tatatcatat catcgtccgg
     4681 cgtctttttt cggcatcgga ccgcgcagaa aaagggaccg aaagggaaac aacgatggca
     4741 aaccgtcgcg cattcgatat cgtccgccgg ggaggcgtcg tggttctatg gctcgctctg
     4801 gccatggttt ttccggggcg cctcttggcg gccgaggcgg ggtcgaagaa aatcaaggtt
     4861 ttcctggtcg gagtggacgg gggcacctgg gggatattga acggattggt ggaggccggg
     4921 gcggctccga acttccggcg tttgatggaa gccggcactt acggcgtctt gaagagcacc
     4981 gagccgatct attcgccggt gttgtggacc tccatcgcca cgggcctggc gccggaaaaa
     5041 cacgggatca cgcatttctt caaggatccc gcccacccgg tccgtcaggt caattcctcg
     5101 gaccgaaagg
//
LOCUS       DAOTFD010000190         5074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11898_length_5074_cov_3.552102, whole genome shotgun sequence.
ACCESSION   DAOTFD010000190 DAOTFD010000000
VERSION     DAOTFD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11898_length_5074_cov_3.552102"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..5030)
                     /locus_tag="P5079_09485"
     CDS             complement(129..5030)
                     /locus_tag="P5079_09485"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRY30251.1"
                     /translation="MTTVDVAQLEGDVVVLEKRGAPGNKLASPPAAVPVVSALHVGTL
                     VRWTRYLSSAHWKGVPFVGAVRDLLAQMPEVDRATKAGLLRWMRRAARGSSAEVLKEA
                     GRYAPLLSAVLGDYLERFVLRPMDAGSPEALPYRPTVAALEREAAIRLEGLAQQGLEG
                     MVSPGKRVIVRVSRAEGLLKNRDLAMAAVFKPQPDGSAVLYVHQRFMDALMAQDGRKR
                     DELVRALVERELSLQQLLMQGHGFDEAHQKISENPTQIQLIDFMKALVGVRLPVALEA
                     ASAEAMRFKPVKEALEVEAVKQLKALSDRGIEGVVAPEKRLWVKIFQETPASLRGAFF
                     PVAESDGSVTLYIHQGLMENLMSQSGRQLDDRVKLIVEHALAIQQLLNEGSTLSEAQA
                     LLATDPPAAVYTREPPAASSADPRLIGRELRVELLKLKELQEASPSAGTIFGGNRISR
                     DSYDYQMAVLLGKKMYQRGIVPRTGGGPGWMEAVLKGYKEARDADIAAGKKPVVAHKT
                     QGFKKPFVGQPLNDYVEVSAFFDQLVTRKMSLIGNSDGLLTAPGGYGTFDEIFDAWRA
                     GRPQVLLGREWEPLIEALYRSWEKFGLLDDNLRKFRPLVVDLTEEGLDQALDYVLSAP
                     RSEEPDLKDLPVKEVDRRGREIEKLLERYADWPPSIKVLGSRDIDLTKTPIGMASFIA
                     RWAADNGMPLQVGGPGPVTRLLGAVLRGVGKNDLLQGIFLNKEMDPVEVDRYFSPDRL
                     IKTDNSIVQKMFQVENARAYVVFPGKLGTLDVLSELLPQMQMGIISERPIVLVGRAFW
                     QPIKDEIVKMMGPRGLLLAGKEDLLQIVDTEEELQDILDTLKPKTPAQMKEEFLARQE
                     ELGRWLEEVSPSAAPAGVSPWWEEIVSVDDAGMGDVPRLLRQIFSDFYWIKVAAAARL
                     SVLMESPDFPRGAEWESLLSGFSPSQREELREWMDLLRTRSKAIAERAEEISKGEQEL
                     LASKLRAAGESVSMEQLQGLLREVQAWGVEQRERFIGGFEADGGLARRLAGWAKIEVG
                     RKDNAFRIGPTFGDAVPLRSIFWWQAVLDAAKWRGRFSGVTAAARRDQVYQYLSDISV
                     METRIEEYQQAKEQGADVLRLAARAANDAENPSEKMRVGSVIVSAAGKVVGAGFNEAP
                     AASGADFRHSGGDPRQKQFMPQDPEEYQRFYRSRYDWRRSPCAEKKAVLNALLRGHSL
                     EGATLYTNLYPCQHCSVFLQGTGIKTIVSPEYTDKEPSGEVIQAGLRHLHAPAAFDSI
                     PNDASRVLEARAEGEYMVDQQFVTMLRDLGASDAGHLPTAALVQTFASNPSLTKLVRF
                     FALRPAPSRPAVPPAPSAPLISRRAVLKGLGGGLVALAAFSPLELLAKALNDPAALVR
                     AAVKDGNLGAAYQKLSPYLADENIVHELIRVLVEKGDVDARRAASYVLTQLPKRTAAV
                     VLGYVRAMGDHEIALNAPLSIYAREAIEKNPELLPAVVEEGTRLGYLRGVFNLLMEVD
                     PAKARTWFNARYGDRFPLVQRLLGRFQENGGFQNPSAVELAAWKKEAADFLNGELKGA
                     SPPGKVLGGADGLFAVTNLLLAILLVQQERFALPPMAFSLPTCPRCSSTHPPQKLASS
                     GCSKSTGNGDRTAWSWRDRSFTT"
BASE COUNT          988 a         1788 c         1369 g          929 t
ORIGIN      
        1 gagacccgtc gcgtgtctgc cgacaacgtc gtctccgtcg atggcgtttc ggatcggcaa
       61 cgagagcagc gcttcgtggg gtcgcgactg aacgtggccc gcctgatact cccggccaag
      121 gagggcgatc atgtcgtgaa ggatcgatcg cgccatgacc acgctgtccg gtcgccgttg
      181 ccggtgctct tcgaacagcc gctcgaagca agcttctgtg gcgggtgagt acttgaacac
      241 cgggggcatg tcggcagcga aaaagccatc ggcggcaacg caaaacgttc ttgctgcacc
      301 aacaggatgg ccaaaagcag gttggtgacg gcaaaaagtc cgtcagcccc tcccaacacc
      361 ttgccgggcg gagaagcccc cttcaactcc ccattgagga aatcggccgc ctctttcttc
      421 cacgccgcca gttccaccgc cgaggggttc tggaaccccc cgttttcctg gaatcgcccc
      481 aacagccgct gaacgagggg aaaccggtcc ccgtaccgcg cgttgaacca cgtgcgggcc
      541 ttggccggat ccacctccat caacaggttg aagacccctc gcaaataccc gaggcgcgtc
      601 ccctcctcca ccaccgcggg cagcagctcg ggatttttct cgatggcctc tcttgcgtag
      661 atggagaggg gcgcgttgag ggcgatctca tggtccccca tggcgcgcac ataccccaaa
      721 acgaccgccg ccgtgcgttt ggggagctgc gtcaacacat acgacgccgc ccgacgtgcg
      781 tccacatccc ccttctcgac cagaacacgg atcaattcgt gaacgatgtt ttcatccgcc
      841 aggtacgggc tcagtttctg ataagcggct cccaggttgc cgtctttgac ggccgcgcgg
      901 accaaggccg ccggatcgtt cagcgccttg gccaaaagtt cgagcgggct gaacgccgcc
      961 aaagcgacga gcccgccccc caatcctttg agcaccgccc ggcggctgat caacggcgcg
     1021 gaaggcgccg gaggaacggc aggccgggac ggcgcggggc gcaaggcgaa aaatcgaacg
     1081 agtttcgtga gtgaggggtt tgacgcgaaa gtctggacaa gggcggcggt gggcagatgg
     1141 ccggcatccg acgcccccag atcgcgcaac atggtcacga attgctggtc caccatgtat
     1201 tctccttcgg cgcgggcctc cagaacacgc gaagcgtcgt tggggatcga atcaaaggcc
     1261 gccggggcat gcagatgccg gagaccggcc tggatcacct cgcccgaagg ctccttgtcg
     1321 gtgtattccg gcgacacgat ggtcttgatg ccggtcccct gcaggaacac cgaacaatgc
     1381 tggcagggat acagattggt gtacaaggtg gccccttcca gggagtgtcc ccgcaagagc
     1441 gcgttgagaa cggccttctt ttcggcgcag ggagaccggc gccagtcgta ccggctgcga
     1501 taaaaacgct gatattcctc cgggtcctgc ggcatgaact gtttctgccg cgggtcgcca
     1561 ccggaatgcc ggaaatccgc gccggacgcc gccggcgcct cgttgaaccc ggccccgacg
     1621 accttgccgg cggcggagac gatcaccgat ccgacgcgca ttttctccga tgggttctcg
     1681 gcgtcgtttg cggcgcgggc cgccaaacgg agcacatccg ccccctgttc ttttgcctgc
     1741 tggtattcct caatgcgggt ttccatgacg gagatatcgg acaagtattg gtagacctga
     1801 tcccgccggg ccgccgccgt aacacccgag aatcgccccc tccacttggc cgcgtccaaa
     1861 acagcctgcc accagaaaat ggatctcaac ggcaccgcgt cgccgaacgt cgggccgatg
     1921 cgaaacgcgt tgtctttccg ccccacctcg attttcgccc acccggccaa acgcctcgcg
     1981 aggccgccgt cggcctcaaa accgccgata aaccgttccc gctgttccac cccccacgcc
     2041 tgcacctccc gcaaaaggcc ctgcagttgt tccatcgata cggactcgcc ggcggcgcgc
     2101 agttttgacg ccaacaactc ctgttcccct ttcgaaatct cctccgcgcg ctccgcgatg
     2161 gctttgcttc tcgtgcgcag gagatccatc cactcccgga gttcttcccg ctgggaaggg
     2221 gaaaaaccgc tcaagagact ctcccactca gcccctctcg gaaaatccgg cgactccatc
     2281 aggacggaca accgggcggc ggccgccact ttgatccaat aaaaatcgct gaagatctgt
     2341 ctcaacagcc ggggcacgtc gcccatcccg gcatcgtcga cggaaacgat ctcttcccac
     2401 cacggagaga cgcccgccgg cgcggcggag ggagacacct cctccagcca ccggcccaat
     2461 tcctcttgcc gcgccaggaa ctcctctttc atctgcgccg gcgttttcgg tttcaacgta
     2521 tccaaaatgt cttgaagctc ttcctccgtg tccacaattt gaagaaggtc ctctttgccg
     2581 gccaaaagaa gaccgcgcgg ccccatcatc ttgacgatct cgtccttgat gggctgccag
     2641 aacgcccgcc ccaccaggac gatgggcctc tcggagatga tccccatctg catctgcggc
     2701 agaagctcag agaggacgtc cagggtcccc aattttcccg ggaaaaccac ataggcgcgg
     2761 gcgttctcca cctggaacat tttctgcaca atggaattgt cggtcttgat gagccggtcc
     2821 ggcgaaaaat accggtcgac ctccacggga tccatttcct tgttcaagaa tatcccctgc
     2881 aacaaatcgt tcttgcccac gccccgcaac accgccccca acaaccgcgt caccgggccc
     2941 ggcccgccca cctgcagagg catgccgttg tccgccgccc atcgggcgat gaacgacgcc
     3001 atcccgatgg gggtcttcgt gagatcaatg tcccggctgc ccaacacctt aatggacggc
     3061 ggccagtcgg cgtaacgctc cagcaatttt tcgatctccc tcccccgacg gtccacttct
     3121 ttcacgggaa ggtccttcag gtccggctcc tcggaacgcg gggcggagag gacgtaatcc
     3181 agcgcttgat cgagcccctc ctcggtcagg tcgaccacga gcggccggaa cttgcgcaaa
     3241 ttgtcgtcca acaacccgaa cttctcccag gaccgataaa gcgcctcaat gagcggttcc
     3301 cattccctgc ccaacaacac ctgcggacgg ccggcccgcc aggcgtcaaa aatttcgtcg
     3361 aacgtgccgt agccgcccgg ggcggtcaag aggccgtcgc tgttcccgat caacgacatt
     3421 ttccgggtga ccaactggtc gaaaaacgcg gacacctcca cgtaatcgtt gagcggctga
     3481 cccacgaacg gctttttgaa tccctgtgtt ttatgcgcca ccaccggttt cttgcccgcg
     3541 gcgatatcgg cgtcccgcgc ctccttgtag cctttcaaca ccgcctccat ccaacccggt
     3601 ccaccgcccg tcctcggcac gattccccgc tggtacatct ttttccccaa aagaacggcc
     3661 atttgatagt cgtaactgtc ccggctgatc cgattgcctc cgaaaatcgt cccggcggag
     3721 ggagacgcct cctgcaactc cttcagtttg agcaattcca cccgaagttc ccggccgatg
     3781 agccgcggat ccgccgacga cgcggccgga ggttcacgcg tataaacggc cgccggcgga
     3841 tcggtcgcca aaagcgcttg ggcctcggaa agggtgctcc cttcatttaa gagttgctgt
     3901 atcgccaacg catgctcgac gatgagcttg acccggtcgt ccagttgacg gccactttgg
     3961 ctcatcaggt tctccatcaa cccctgatga atatagagcg tcacggaccc gtccgactcc
     4021 gccacaggga agaaagcgcc ccgcaaagac gccggcgttt cctggaaaat cttcacccag
     4081 agtcgtttct ccggcgccac caccccctca atcccccggt ccgacaaggc cttcaattgt
     4141 ttcacggcct ccacttccaa cgcttctttc accggcttga accgcatcgc ctccgcggac
     4201 gccgcctcca gcgcgaccgg caaccggaca ccgaccagcg ccttcataaa atctatcaat
     4261 tgaatttgtg tgggattttc ggagatcttt tggtgggctt cgtcaaatcc atgtccctgc
     4321 atcaaaagct gttgaagcga caactcccgc tcgaccaaag cgcggacgag ttcgtccctc
     4381 ttccggccgt cctgggccat cagagcgtcc atgaaccttt ggtggacgta aagcaccgcc
     4441 gaaccgtccg gttgaggttt aaaaacggcg gccatggcca ggtcccggtt tttcaacagc
     4501 ccttccgctc gggacacccg cacgatcacc cgctttcccg gcgaaaccat tccctccagg
     4561 ccctgttgcg ccagcccctc caagcggatt gccgcctcac gttccagcgc ggccaccgtc
     4621 ggccgatagg gcaacgcctc cggagaaccc gcgtccatcg gccgaagcac gaacctttcc
     4681 aggtaatccc ccaacacggc cgacaggaga ggcgcgtacc gcccggcctc cttcaacacc
     4741 tcggcggacg atccgcgcgc tgcacgccgc atccatcgca aaagacccgc cttggtcgcg
     4801 cggtccacct ccggcatctg ggccaatagg tcccgaaccg cccccacgaa cggaaccccc
     4861 ttccaatgag cggacgacag ataccgcgtc catcggacca acgtccccac gtgcaaggcg
     4921 ctgacgaccg gcacagcggc cggcggcgac gccaatttgt tccccggggc gcccctcttc
     4981 tccaaaacca cgacatctcc ctccaactgc gccacgtcaa ccgtcgtcaa atctacttct
     5041 tcgtgcgtgc ccttctgcca tccctcttcc agcg
//
LOCUS       DAOTFD010000191         5063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_11938_length_5063_cov_2.881190, whole genome shotgun sequence.
ACCESSION   DAOTFD010000191 DAOTFD010000000
VERSION     DAOTFD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_5063_cov_2.881190"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5063
                     /locus_tag="P5079_09490"
     CDS             <1..>5063
                     /locus_tag="P5079_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30252.1"
                     /translation="FASPVLWIASAGMVFGYLFYFQGLRYGGATISSLLLEGWAPVAG
                     AIITCALSARLPLAGEIVSFLLILAGVSTLSSHLGRGPPAEKVKPVGTNGATRVKLGV
                     SIGTASVRVGYISVDDKGGILGEGDKCVIHSVSADIPRGANGLTDVAEAVRIAGNLLR
                     RSRLDVEAEDWKIVKGVGVSAPGAYLNDGSVYPGTVPNIPGLENIRLHEALQKEFGGD
                     FTVAPANVNNDGVLQGLVAASYYVRSHPDFEGRKIAVLVPGVGFGAGIYSVEEGEVAP
                     LPGPQQFYDVVVRNATPGETVVVRQGEKTEVRLARGGEPIIMDDIVSGRALELLAGVR
                     LGRSVTIHELNEMALEGDEAAREVFRIAGRALARFMIRMNTGTFKKGVVDYRPDTVGV
                     ELFLIGGAWMPTGGAREFLLEAAEEHLKEKGAAHLRIVRADEVRGMERLTGHLGVIGA
                     TLLAHETDGSVIFADRSPASRWRRFLQFLRLRAPPAPVPTVVKPEPVPAPAWSFYNQA
                     SVDRLQDLLTGRLRDRGADRPLVVAVDGEENVGRIQLAESLSLPARTKVIHLEDFAVP
                     LEEPSGDYATYVEQFNMPVPKQGWDWDELWEAIEQAARAGYEVILVEGIGVLNAERRD
                     WRFDVRVNVIADDRISRIFQERAGVGPVSRDPYYRFRYATRPNYDMVVENVHPGSSAV
                     PAAGAPLPVHTNGATRVKVGVDIGGTSLRVGFVSVDDEGRIVGQGEKCLVYAVSQKMP
                     RTETGLADGPEAVKWLGRMILEAVEEVEHAGWTVSRRLGVSAPGAYLEDGNVYPGTVR
                     NVPSLEKMKLYEALGAELGPDWDLSPASVNNDGVLQGQVAAAKYLETDRTFRDGKIVS
                     FVPGTGFGAGAYEVKDGVVLPLPGPQQLCDVVVGAASSGEPILVQEGESLVVRPAEGG
                     EPLSVENLVTGRALGLLGSAAFGRAVTGGELSRLAMDGDPRASRIFAHAGVALARFAM
                     KINSGDFEKTTVAHRPDTRGAAVFLAGGSWLLHGAGEKITLRVMRETLDQNGRGDIKI
                     VRIDELPGMEGLIPHLGILGAASLAPFDRDVPLAETAGAETPPLPAAGTEGEVSSAAS
                     RWFQRFVRSPRRLFSPWKTPAVPVSSAVEGPAPVDVSAPESAVPAADTAPPSVAWPVY
                     NAAALEKLRRFIGERLEQRGPAAARPLLVAIDGDSDVGKTEFSERLNLNARTKILHID
                     DFYHEVPPASGDYLAYVEQFDYPVLEEYWDFPAVAREVSQAMNSGLYDVVIVEGLKVL
                     KSELDDVRFDVRVNVTADMRTRDLFHRMKQRMGGEQVARRLTSYLEKYSKQYLDGERK
                     YDVVVDNSIPADFTEEDLLRLASQEDIGAAHELKGPPLAALRSLYERLLRLAETLGME
                     GVPLPESEELSEAPAVMPTEELTRRLDLLDRMERLRVGPETWHWAKDPLRLGRFVRMH
                     RDVLLALVDAVSRRFVAALGDREVALIRRAARNLSGAEFERLSRDGRQAVFRLRSSDG
                     GTVRVAVQAALGLPMHAFFADDGTHVGATIQGPPSRRAVFRRLADLASFRIFRRSAYT
                     ITLQPDVLRHPARLSRALQEEFLEIRLHRRGFSFARAHEQVHARLAAAGLEDDLPRQL
                     EEGKDRYAALVPELERTSGQLNETVAAARAAVDDRFRHNRSFLAALALFSESGGIEMT
                     EEGIVSSSPESADFAALLN"
BASE COUNT          823 a         1492 c         1797 g          951 t
ORIGIN      
        1 gttcgcgtcg ccggttttgt ggatcgccag cgccggcatg gtgttcggct atctgttcta
       61 ttttcagggg ttgcgatacg gcggcgccac catcagcagt ttgttgctgg aaggctgggc
      121 tccggtggcg ggcgccatca tcacctgcgc gctcagcgcg cgcctcccgt tggccgggga
      181 gatcgtctct ttcctgttga tcttggccgg cgtttccacc ctttcctctc atctcggccg
      241 cggcccgccc gcggaaaaag tcaaacccgt cggcaccaac ggcgccaccc gggtgaaatt
      301 gggggtgtcc atcgggaccg cctccgtgcg cgtggggtac atcagcgtcg acgacaaagg
      361 cggcatcctg ggggaggggg acaaatgcgt catccacagc gtgagcgcgg acatccccag
      421 gggagccaac ggtttgaccg atgtggcgga ggcggtgcgc atcgccggca acttgctccg
      481 acggagccgg ctggacgtgg aggcggagga ctggaagatc gtcaagggcg tgggggttag
      541 cgctcccggg gcctatttga acgacggcag cgtttatccc ggcacggtgc cgaatatccc
      601 cggactggag aatattcgat tgcacgaggc gttgcagaag gagttcggcg gcgattttac
      661 cgtggcgccc gccaacgtca ataacgacgg cgttctgcag gggcttgtgg cggccagcta
      721 ttacgtccgc tcccatcctg attttgaagg gcggaagatc gcggtcctgg tgccgggggt
      781 ggggttcggc gcgggaatat acagcgtgga agagggggag gtggctcccc tgccgggtcc
      841 gcagcagttt tacgatgtgg tggttcggaa cgccacgccg ggggaaaccg tggtggtgcg
      901 gcaaggcgaa aaaaccgagg tccgtctcgc ccgcggcggc gaaccgatca tcatggacga
      961 tatcgtgtcc ggccgggcgc tggaattgct ggctggcgtg cggctggggc ggtccgtgac
     1021 catccacgaa ttgaacgaga tggccttgga gggcgatgag gccgcccggg aggtttttcg
     1081 tatcgccggc cgggcgttgg cccggttcat gattcggatg aacacgggga cgttcaagaa
     1141 gggggtggtg gactatcgtc ccgacaccgt gggcgtcgag ctgttcctca tcgggggggc
     1201 ctggatgccc accggcggag cccgggaatt cctgctggag gccgccgaag aacatctgaa
     1261 ggaaaaaggg gccgctcacc tgcggatcgt ccgcgcggac gaggtccgcg gcatggagcg
     1321 gttgacgggt catctgggcg tcatcggagc gacgctcttg gcgcatgaga ccgacgggtc
     1381 cgtcattttc gcggaccgat cgcccgcttc tcggtggcgg cgtttcctcc agttcctccg
     1441 gctgagggct cctcccgcgc cggtgcccac cgtcgtcaaa ccggaacccg ttcccgctcc
     1501 cgcgtggtct ttttataacc aggcgtccgt ggaccggctt caggatcttc tgacgggacg
     1561 gctccgtgat cgtggggcgg accgtccttt ggtggtggct gtcgacggtg aggagaatgt
     1621 ggggcgaatc cagttggccg agagcctgtc cctgccggcg cgcaccaaag tgatacacct
     1681 cgaggatttc gccgtcccgc tggaagagcc ttccggggac tacgcaacgt atgtggagca
     1741 gttcaatatg cccgtgccga agcaggggtg ggactgggac gaactctggg aggccatcga
     1801 acaggcggca cgggccggtt acgaggtgat cctcgtcgag gggattgggg tgttgaacgc
     1861 ggaacgaagg gattggcgtt tcgatgtgcg cgtcaacgtc atcgccgatg atcggatcag
     1921 ccggatattt caagagaggg cgggcgtcgg tccggtttcc cgcgatccgt attatcggtt
     1981 ccgctatgcc actcgtccga attacgacat ggtcgtcgaa aacgtccatc ccggttcttc
     2041 cgccgtcccg gcggccggcg cgcctttgcc ggtgcatacc aacggggcca cgcgggtgaa
     2101 ggtgggcgtg gacatcggcg gcaccagcct gcgcgtgggt ttcgtcagcg tggatgacga
     2161 agggcgtatc gtggggcagg gcgaaaaatg cctggtctac gccgtttcac agaaaatgcc
     2221 gcggaccgag accgggctcg ccgacggtcc cgaggcggtg aaatggctcg gccggatgat
     2281 ccttgaagcg gtggaggagg tggagcacgc gggctggacc gtgtcccgac ggctgggcgt
     2341 gagtgcgccg ggggcctatc tggaggacgg caacgtctat cccggcacgg tgcgcaatgt
     2401 gccgtcgctg gaaaagatga aactttacga ggcgttgggg gccgagttgg ggccggactg
     2461 ggacctctct cccgccagcg tgaacaacga cggcgttctg cagggacagg tggccgcggc
     2521 caaatatctg gagacggaca ggacgttccg ggacggcaag atcgtctctt ttgtgccggg
     2581 caccggtttc ggcgccgggg cgtacgaggt aaaagacggc gtcgtcctcc ctctgccggg
     2641 acctcaacag ctttgcgacg tggtggtggg ggccgcgtcg tccggggaac ccatccttgt
     2701 tcaggagggc gagtccctgg tcgttcggcc cgccgaggga ggggaacctc tcagcgtgga
     2761 aaacctcgtc accggaagag cgctgggtct gttgggaagc gcggcgttcg ggcgcgcggt
     2821 gacgggggga gaattgagcc gtttggcgat ggacggagac ccgcgggcct cccgaatttt
     2881 tgcgcatgcc ggggtggcgc tggcgcggtt cgccatgaag atcaattccg gagatttcga
     2941 gaagaccacc gtggcccatc gtccggacac ccgcggcgcc gccgttttcc tggccggcgg
     3001 ttcctggctc cttcacggcg ccggcgagaa gatcaccctg cgcgtgatgc gggaaacgct
     3061 ggaccaaaac ggccgtgggg acataaaaat cgttcggatc gacgaattgc cggggatgga
     3121 gggattgatc ccccacctgg gcattttggg cgcggcgtcg ctggcgccgt tcgatcgtga
     3181 cgtgccgctc gcggaaaccg ccggagcgga aacgcctcct ttgccggcgg cgggaacgga
     3241 aggggaggtt tcctctgccg cttctcggtg gttccaacgg ttcgtgcgtt cacctcggcg
     3301 gctgttctct ccctggaaga cgccggcggt tcccgtgtcg tccgcggtcg aagggcccgc
     3361 gcccgtcgac gtttcggccc ccgaaagcgc cgtcccggcg gcggatacgg ccccgccctc
     3421 cgtcgcctgg ccggtctata acgcggcggc gcttgaaaaa ctgcggcggt tcatcggcga
     3481 acgcttggaa caaaggggtc ccgctgcggc tcggccgctc ctggtcgcca tcgacggcga
     3541 ctcggatgtg ggaaaaacag agttcagcga gcgcctgaac ctgaacgccc gcaccaagat
     3601 cctccatatc gacgattttt atcatgaggt gcctccggcg agcggggatt acctggcgta
     3661 tgtcgagcag ttcgattatc cggtcctcga ggaatactgg gattttccgg cggtggcgcg
     3721 ggaggtgtct caggcgatga attccggtct ttacgacgtc gtcatcgtgg agggattgaa
     3781 ggtgttgaag agcgaactcg acgacgttcg gttcgacgtg cgcgtgaacg tgacggccga
     3841 tatgcgcacc agggacctgt tccaccgcat gaaacagcgg atgggaggag agcaggtcgc
     3901 gcggcggttg acgtcctatt tggagaagta ttcaaaacag tatttggacg gtgaaaggaa
     3961 atacgacgtg gtggtcgaca acagtatccc ggcggatttc accgaggagg acctgttgcg
     4021 cctggcgtcg caggaggaca tcggggcggc ccatgaattg aaaggtccgc cgctggcggc
     4081 gttgcggtcg ctctacgagc ggctcctgcg gttggcggag acgttgggaa tggaaggggt
     4141 gcctttgccg gagtccgagg aactttccga ggcgccggcc gtgatgccga cggaagaact
     4201 gacgcgccgt ctcgacctgt tggatcgcat ggaacgcctc cgcgttgggc cggagacgtg
     4261 gcattgggcg aaagaccctc tgaggttggg acgtttcgtg cgaatgcacc gggatgttct
     4321 gctggcgctg gtggatgccg tctcccgccg ttttgtggcc gccctcggcg accgggaggt
     4381 ggcgttgatc cgccgggccg cgcggaacct gtccggggcg gaattcgaac gtctttcccg
     4441 ggatggccgt caggcggtct tccggctgcg ttcttcggac ggcgggaccg tccgcgtcgc
     4501 ggtgcaggcc gcgctgggcc taccgatgca cgctttcttc gccgacgacg ggacccacgt
     4561 cggcgccacc atccagggcc ccccttcccg ccgcgctgtt ttccggcggc tcgccgatct
     4621 ggcgtctttc cgtattttcc gacgcagcgc ctacaccatc acccttcaac ccgacgttct
     4681 ccgtcatccc gcccggctct cccgagccct ccaggaagaa tttttggaaa tccggctcca
     4741 tcggcggggt ttttctttcg cccgcgcgca cgaacaggtc cacgcccgcc tggccgcggc
     4801 cggcctggag gacgacctcc cccgccagct ggaagagggg aaagaccggt acgccgccct
     4861 cgttccggaa ctggagagga cctccgggca gttgaatgaa accgtcgccg ccgcccgagc
     4921 cgccgtggac gaccgcttcc ggcacaaccg aagtttcctg gcggcgctgg cgctcttcag
     4981 cgagagcggg gggatcgaaa tgaccgagga ggggatcgtc tcctcttctc cggaatcggc
     5041 ggatttcgcc gcgctcttga acg
//
LOCUS       DAOTFD010000192         5030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12045_length_5030_cov_4.454070, whole genome shotgun sequence.
ACCESSION   DAOTFD010000192 DAOTFD010000000
VERSION     DAOTFD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12045_length_5030_cov_4.454070"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5030)
                     /locus_tag="P5079_09495"
     CDS             complement(<1..>5030)
                     /locus_tag="P5079_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRY30253.1"
                     /translation="DQPQTPVQQMAEKAGDTAVAIAKASTPNVLETPLADVGEAAAEA
                     ISETKRLLFHISLTSEHKKAKKAQLAEIDKKERQLQDAIYKKHLDYFGRHPEWEKILQ
                     AARDQVRAELTAAREAYEKQKADAQKAGQTLPEKGLSVINPWGLMFFHDRFADRLGGA
                     QRKNIEVRGRDLTSGWRGVVHIVTSQQTPQSNADAKEAAEHFGIDAEEMKKLAGKEVG
                     TVELFHVLEFTPDIQGFAKWVAFKEDAEAAEDERAAHQVFFPWVPAWGPRYGLVFANP
                     QPLAEPKDAKGALGLFDWNPVQQAAEGETAQAGAEAGTPDEWETFLADWTTRLNEADR
                     KLKSTVRGSLKMVRYLAHQRGLEGAQARKIENDLLSLLQTAQSGVQERLTQLYREMGG
                     RQGAGAQDRIAISITPEGKVQSFPVKASLDSAASPLTEKKTANRLAVVSGGEMVLAFT
                     DQERVYVVSGDTLPLEKGEKVTFVTSLNMMGVGKKYVVLATKNGRVRRMAIDKIIPYA
                     KSKQRIFSFKEEGDEILSLTSTDGDQELLLISRNGQALRFHEKDVPEHVTEATQGVRG
                     IQLPDGDALVSMTATASPNQTALVLLEDGTAKRVKVDGFPFFDRNQIVYRTPRKWQEM
                     VGDRIISRSRVAGVYLVDDSDQILSVTQGRLVRKNVADLSVMTSPFDAASPFVDVPAG
                     EKVLMSAVIPRGEDTTGTSEQDLAQWQEKFQTAMREAQAAGREAANLWKERIERARKR
                     SFPAEEHPTKVISVKQGPERWADQIAGGLKTIETREWKDTTIVGKTVYLHVPKQRGDR
                     HGRIIGKFRVIDVVDLSNPDVFAALQSMHRVPYFEFGSRFGYVLGEIETLDEPMPYPG
                     GQRFFEFDEKVAEEAAQEEEEAQEEAAARKEAGEGPLGGGPTSGKAPGGDDPLVATGM
                     DYDLEAANEIRSALNTLDGLAKLKDGVKNGHILLESLGEYLDQALKQKAPADIASIEK
                     ALVEFAEATSEADPAKITAASQKIRELAAASEENWEFIEPLLSSWGIAPEAKEELLGD
                     RPATIEEKMAEKVQEKIAPKQRKTPTKKSSKPALYESLEVLRPPVMQTYKPIRRLRLV
                     DENGTATPLSFDGRSGDNLRLTLTVGGKKIAITSDEAEEVLLIAAGYMTPMKDNDPAQ
                     IDREHLPKVSSERNLMLGLPDVDPQTKQKRVPGLLDVLRKAGIDLPEDTPFYKNRATG
                     EVSEVFADVARFVTHYRTVKKQLEKSPKADKTPNVRIVPAAEEGEEEDAEREPIEMYV
                     SFNNAESLGRWGVYRAHSRPKFDGLLRPPVGLSGLSLNATATPEGWPKAQTESDPTKL
                     QVVSWHHPVTGAKKGIRLGSQYATQQDIKKYLKADLLGANVQEILDGAWETDEAYQKY
                     NAGYLDKKSYLKYVREVKDKIRPWAKRLGTDKDMRVIDLIRKKIAQMILSNNTADQQI
                     GIVFYLMDTMHARIGGESTSATEAVGITKWRADQLEFLSDFVLHWRFIGKSGMLWDDT
                     RQLTNPAVEDDYTIDPKKFELYYTDAVNKRVYEILKKRAEQGLQNLARSKKTAEQLVK
                     EGNESDIEVFSINADNVNDFLKQFGPFTAKYTRPFLAGMYARHRLSLLEKSLESAKKA
                     DLKKVIEGDQDKNTGQFVERDAKGKITEDRRGVLPFVGEKLGHRRVNKKAGDRVEITG
                     STERVNYV"
BASE COUNT          713 a         1502 c         1611 g         1204 t
ORIGIN      
        1 ccacgtagtt gacgcgctca gtggagcccg tgatttccac ccggtcgccg gcctttttgt
       61 tcacgcggcg atggccgagt ttttctccca cgaacggcag cacgccccgc cggtcctccg
      121 tgatcttgcc tttcgcgtcc cgttcgacga actgccccgt gttcttgtcc tggtccccct
      181 cgatcacctt cttgaggtcc gctttcttgg cggactccag agacttttcc aagagcgaga
      241 ggcggtgccg ggcatacatg ccggccagga acggccgggt gtatttggcg gtgaacggcc
      301 cgaactgctt caggaaatcg ttgacgttgt cggcgttgat ggaaaaaacc tcgatatcgg
      361 attcgttccc ttctttgacc agttgttcgg ctgtcttctt cgaacgcgcc aggttctgca
      421 ggccctgttc cgcccgtttt ttgaggatct catagacccg cttgttcacg gcgtcggtgt
      481 aatacagctc aaattttttg gggtcgatgg tgtaatcgtc ttcgacggcg gggttcgtca
      541 gttgccgcgt gtcgtcccac agcatgccgg acttgccgat gaagcgccag tgcaagacga
      601 aatcgctcag gaattccagc tggtcggcgc gccatttggt gatgcccacc gcttcggtgg
      661 cgctggtgga ttcgccgccg atgcgggcgt gcatggtgtc catgaggtag aacacgatgc
      721 cgatctgctg gtccgcggtg ttgttggaga ggatcatctg ggcgatcttc ttgcggatga
      781 ggtcgatgac ccgcatgtcc ttgtccgtcc cgagccgttt cgcccacggg cggattttgt
      841 ctttgacttc ccgcacgtat ttcaaataac tctttttgtc cagatagccg gcgttgtatt
      901 tctgataggc ttcgtccgtc tcccacgcgc cgtcgaggat ttcttggaca ttggcgccca
      961 agaggtccgc tttgaggtat ttcttgatgt cctgctgggt ggcgtattgg ctgccgaggc
     1021 ggatgccttt cttggcgccg gtgaccggat ggtgccagga aacgacctgg agtttggtgg
     1081 ggtcgctctc ggtctgggct ttcggccatc cttccggggt cgccgtggcg ttcagcgaca
     1141 gtcccgacag tcccaccggc ggtcggagga ggccgtcgaa tttgggtcgg gaatgggcgc
     1201 gatagacgcc ccagcggccc agggattccg cgttgttgaa cgagacatac atctcgatcg
     1261 gctcgcgctc cgcgtcctct tcttcccctt cctcggcggc ggggacgatc cgcacgttgg
     1321 gggtcttgtc ggccttgggg gatttctcga gctgtttctt gaccgtgcgg taatgggtga
     1381 cgaaccgggc gacgtcggcg aagacttcgg agacttctcc ggtggctcgg tttttataga
     1441 agggcgtgtc ttccggaagg tcgatgccgg ctttgcgaag gacgtccaaa aggccgggca
     1501 cgcgtttctg tttcgtctgc ggatccacgt ccggcaggcc cagcatcagg ttgcgttcgc
     1561 tggagacttt gggcagatgc tcccggtcga tttgggcggg atcgttgtcc ttcatgggcg
     1621 tcatgtagcc cgcggcgatg aggagcacct cctcggcctc gtccgaggtg atggcgatct
     1681 tcttgccccc gacggtgagg gtcagacgca ggttgtcgcc gctccggccg tcgaaggaga
     1741 gcggggtggc cgtgccgttc tcatccacca ggcggagccg acggatgggc ttgtaggttt
     1801 gcatgaccgg cgggcggagg acctcgaggg attcgtagag agccggcttg gaggattttt
     1861 tcgtcggcgt ttttcgttgt ttgggggcga tcttctcctg gaccttttcc gccattttct
     1921 cctcgatggt ggccggccgg tctcccagga gttcctcttt cgcttccggc gcgatgcccc
     1981 aggaggagag gagcggctcg atgaactccc agttctcctc gctggcggcc gccagctctc
     2041 ggatcttctg ggaggccgcg gtgatcttgg ccgggtccgc ctcggaggtg gcttcggcga
     2101 actccaccag ggctttttcg atggaggcga tgtccgccgg cgctttctgt ttcagcgcct
     2161 ggtccaaata ttcacccagg gattccagga ggatgtgccc gttcttgacg ccgtctttga
     2221 gtttcgccag tccgtccagg gtgttgagcg ccgagcggat ttcgttcgcc gcctcaaggt
     2281 catagtccat tccggtggcc acgaggggat cgtcgccgcc gggggctttg cccgatgtcg
     2341 gaccgccgcc cagggggccc tctccggcct ctttccgcgc ggccgcctct tcctgggctt
     2401 cttcttcctc ttgagcggct tcctcggcga ctttttcgtc gaattcgaag aagcgttgtc
     2461 cgcccggata aggcatcggt tcgtccagcg tttcgatctc gcccaggacg tatccgaagc
     2521 ggctgccgaa ttcaaagtag gggacgcggt gcatggactg aagggccgcg aagacgtccg
     2581 gattgctgag gtccaccacg tcgatcacgc ggaacttccc gatgatccgg ccgtgccggt
     2641 ctccgcgctg cttgggcacg tggaggtaga cggtttttcc gacgatcgtg gtgtctttcc
     2701 actcccgggt ctcgatggtc ttgagtcctc cggcaatctg gtcggcccag cgttccggtc
     2761 cctgcttcac gctgatgact ttggtgggat gttcttccgc cgggaaggaa cgtttgcggg
     2821 cccgttcgat ccgttccttc cataagttcg cggcttctct ccccgcggcc tgggcctccc
     2881 gcatggcggt ttggaacttt tcctgccatt gggccaaatc ttgttcggag gttccggtcg
     2941 tgtcttcgcc gcgcgggatg accgccgaca tgagcacttt ttcgcccgcc ggcacgtcca
     3001 cgaacgggga ggcggcatcg aaaggagaag tcatcaccga caggtccgcc acgttcttgc
     3061 gcaccaggcg gccctgcgtc acggacagga tctggtcgct gtcgtccacc aggtagactc
     3121 cggccacccg gctgcgggag atgatgcggt cccccaccat ctcctgccat ttgcgcggcg
     3181 tgcggtagac gatctggttc cggtcgaaga acgggaagcc atccaccttc actcgtttgg
     3241 ccgtgccgtc ctccagaagc accagggccg tctggttggg agaggcggtg gccgtcatgg
     3301 agaccagcgc gtcgccgtcc ggcagttgga tgccgcgaac gccttgggtg gcctcggtga
     3361 cgtgttccgg aacatccttt tcatggaagc gcagggcctg tccgtttctg gaaatcagca
     3421 gcagttcctg gtctccgtcg gtggaggtga gagagaggat ttcgtcccct tcttccttaa
     3481 aggaaaagat gcgctgcttg cttttggcgt aagggatgat cttgtcgatg gccatgcgac
     3541 gaacgcggcc gttcttggtc gccagtacca cgtatttctt gccaacgccc atcatgttca
     3601 agctggtaac gaaagtgacc ttctccccct tctccagcgg gagggtgtcg ccggagacga
     3661 cgtagacccg ttcctggtct gtgaacgcca gcaccatttc gccgccggaa accaccgcca
     3721 gccggttggc cgtcttcttt tccgtcagcg gagaggccgc ggaatccagg gaggccttca
     3781 cggggaaact ttggactttc ccctcggggg tgatggagat ggcgattcgg tcctgcgctc
     3841 ccgcgccttg gcgcccgccc atttcgcgat agagctgggt gagacgttcc tgcacgccgg
     3901 actgggcggt ctgcagcagg gacaacagat cgttctctat tttccgcgcc tgggcccctt
     3961 ccaggccgcg ttggtgggcg aggtaacgga ccatcttcag cgaaccgcgc acggtggatt
     4021 tcagtttgcg gtcggcttcg ttcaggcggg tggtccagtc ggccaggaac gtctcccatt
     4081 cgtcgggggt gcccgcttcg gcgcccgctt gggcggtttc cccctcggcc gcctgttgga
     4141 ccgggttcca atcgaagagc cccagcgcgc ctttggcgtc tttgggttcg gcgagcggtt
     4201 gggggttcgc gaagacaaga ccgtaacgcg ggccccaggc cggcacccac gggaagaaga
     4261 cctggtgggc ggcgcgttcg tcctccgccg cctcggcgtc ctccttgaaa gccacccatt
     4321 tggcgaaccc ctggatgtcc ggggtgaatt cgagcacgtg gaagagttcc accgtgccga
     4381 cttccttccc ggccagtttt ttcatctctt cggcgtcgat gccgaaatgc tccgcggctt
     4441 ccttggcgtc ggcgttggac tgcggcgttt gttgggaggt gacgatgtgc accacgccgc
     4501 gccagccgct ggtgaggtcg cgtccgcgga cttcgatatt tttccgttga gcgccgccga
     4561 gacggtcggc gaaccggtcg tggaagaaca tcagccccca cgggttgatg acgctgagcc
     4621 ccttttcggg aagagtctgt ccggctttct gggcgtcggc tttctgtttt tcgtacgcct
     4681 cccgggccgc cgtcagttcc gctcggacct ggtcccgcgc ggcttgaagg atcttttccc
     4741 attccggatg ccggccgaaa tagtccaaat gttttttgta gatggcgtcc tgcagctgcc
     4801 tctccttctt gtcgatttca gccaactgcg cttttttcgc cttcttgtgt tcgctggtga
     4861 gggagatgtg gaacaacaga cgcttggtct ctgagatggc ttcggcggcg gcctccccga
     4921 catcggcgag aggtgtttct aaaacgttgg gagtggacgc cttggcgatg gcgacggccg
     4981 tatcgccggc tttttccgcc atttgttgca ccggggtttg gggctggtcc
//
LOCUS       DAOTFD010000193         5015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12093_length_5015_cov_4.496976, whole genome shotgun sequence.
ACCESSION   DAOTFD010000193 DAOTFD010000000
VERSION     DAOTFD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12093_length_5015_cov_4.496976"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(385..515)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            769..1797
                     /locus_tag="P5079_09500"
     CDS             769..1797
                     /locus_tag="P5079_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY30254.1"
                     /translation="MKAIFFKEHGGADKLIYGDRPTPRPAPHEVLLRVKACALNHLDI
                     WVRAGIPGLPIPLPHIPGSDVAGVVEKTGALVTTVSEGDKVMVAPGLSCGECDDCLLG
                     QDHLCSTFDILGQTHDGGCAEYIAVPAANLIPIPDPLGFEEAASFPLVFLTAWHMLVT
                     LAKIKPGETVLVHAAGSGVGIAAIQVAKLWGARVLTTVGTDAKAAKAKALGADEVINY
                     AKKDFAKETRALTDKRGVDIVVEHIGQDTFEKSVLSLARNGRLVTCGSTSGRQIQFDL
                     RTFFSRNLTIHGSRMGTRRGLDEAVRFLAAKRLRPVVDKVFPLKKAAEAHVYMEERKN
                     FGKIVLSV"
     gene            1843..2253
                     /locus_tag="P5079_09505"
     CDS             1843..2253
                     /locus_tag="P5079_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30255.1"
                     /translation="MNVVIAVTVASIIWFVLAGALFFNPLVEPIYNSEENSPAVKKLP
                     KTPKTMGLIMAAILAQTILWALVFKWLSPHLYWPPLSKGLFFGLMLTCMKMVPRDVDR
                     LLLTTYPLKRMAVEFIIGTACSFVIGLTFAFIIR"
     gene            2384..2617
                     /locus_tag="P5079_09510"
     CDS             2384..2617
                     /locus_tag="P5079_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY30256.1"
                     /translation="MPLFEYYCRKCKKDFEVLHLAGRETKAACPSCSSTDIIQKVSVF
                     SAHSAAPSGSSVGPANPGCRGCPNAGGACGLDG"
     gene            2738..3775
                     /gene="amrS"
                     /locus_tag="P5079_09515"
     CDS             2738..3775
                     /gene="amrS"
                     /locus_tag="P5079_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRY30257.1"
                     /translation="MSLDLTRLTRPGALFEKLDGGNVRCFACGHRCLIADGREGVCRV
                     RFNRGGTLFVPHGYVSALHTDPVEKKPFFHARPGAGAFSFGMVGCDFHCDYCQNWEIS
                     QIFRDPAAVGTVRPMPAAELLRAALRSGAQILASTYNEPLITSEWAAELFREAKKRGL
                     LTAYVSNGNATPEVLEYLKPCLDLFKVDLKSFSEDHYRRLGGDLSKVLDSLRRIHEMG
                     FWLEVVTLVVPGFNDADDELKKIAGHLAALSPDIPWHITAFHQDYKRLSSDNTPVETL
                     VRAAKIGRREGLRYVYAGNLPRMVGDYENTYCPDCRELLVERRGFEVRKNRLAADGKC
                     PQCATAIPGRW"
     gene            complement(3780..4664)
                     /locus_tag="P5079_09520"
     CDS             complement(3780..4664)
                     /locus_tag="P5079_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY30258.1"
                     /translation="MEWLKREQHFYTRRARTVGLLVFFLAGRPLAAVSGRSQADIEYG
                     KAGEDRLLLDVSGPAGPGPFPVALLVHGGGWTTGDKEGEIARLAEFLGAEFTCFSVNY
                     RLAPKYRWPACFDDVQTAVRWVKAHAAEYGGDPRRVALIGYSAGGHLVSCAAVRSTEE
                     TRVGAVVGLAPPTDLEADSERRGKVSRALQALLGLSSDGLDEKARAGLREISPSRRVR
                     PDLPPFLLVHGTGDRSVAFEQSLRFQEKLRAAGVSCDLLPLPGAPHPMGDWEKSDPAY
                     KEKIARWLKETLGPAPRP"
     gene            4782..>5015
                     /locus_tag="P5079_09525"
     CDS             4782..>5015
                     /locus_tag="P5079_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRY30259.1"
                     /translation="MTPPRPPKILAVVGSPRKNGNTHILVSKLLESASTAGSQAETVF
                     LGDLTLKECDGCHACWKGKPCAKKDDMLGLYPKI"
BASE COUNT         1027 a         1646 c         1356 g          986 t
ORIGIN      
        1 cttttaactt taaaccgctc tttagggtcc gtggctttgc gtccccgcct ttcgacgtcc
       61 ctttccactc acttcatgca agggacgtgc cggcctctcc gatgatgaaa gagaaacggc
      121 acgggtttgc cccctggagc gaagcaggag tgagttcggg acctgcgggc cgaagcgatt
      181 tgcctcagcc cgtcctccgg actccgtcct gatttttcca gggccccccg gttaaagggg
      241 atttatcgag cggatgaaaa gatcagcaca ccgtttccca caacctgtct gaaaaacaaa
      301 gaaaccaccg gcacgcaaaa gtacgggtgg tttccgataa ccgctgaatc aaacgcctat
      361 gcgccgaagc gcacatgctc ttgagtcggc agctcggccg gcctccgatt cgcatcggag
      421 cgcccccggc tttcgccgga gacgtagaga ttctcttcct tagccccgtt aactttgcgc
      481 cccacaactt tcgtcgtggg ttgcctttgt cgcaccctaa gtttaagctc tgatcccgtt
      541 ttgtcaaggg ttttttttga actatttttt ttcaggggat ttaaggcaaa aaacgcattt
      601 ttaggggtta tttcaaataa atgcgttata ttacttattt tctagtgaaa tctgtcacaa
      661 acttccctca aaaattgatt tcccccccga aattttgtac attgagcccc tcaaaactca
      721 acaccggcaa acgttgtgga agcaaatact cctttcggag ccagcggcat gaaagcgatt
      781 tttttcaaag aacacggcgg cgcggataaa ctcatctacg gcgaccgacc gaccccccgc
      841 cccgctcccc acgaagtcct cctgcgcgtc aaagcctgcg ccctcaacca cctggacatc
      901 tgggtgcggg ccggaatccc gggcctcccc atccccctcc cccacatccc cggcagcgac
      961 gtggcgggcg tggtggaaaa aacaggggct ctggtcacaa cggtctccga aggagacaaa
     1021 gtcatggtgg ctcccggcct ttcgtgcggg gagtgcgacg actgtctcct gggacaggac
     1081 catctctgct ccacgttcga catcctcggc caaacgcacg acggtggctg cgcggaatat
     1141 atcgcggtgc ccgccgccaa cctcatcccg atcccggacc ctctcggttt cgaagaagcc
     1201 gcctcgttcc ccctggtgtt tttgacggct tggcatatgt tggtgacgct ggcgaaaatc
     1261 aagcccgggg agaccgtgtt ggtgcacgcc gccggaagcg gggtgggcat cgcggccatt
     1321 caggtcgcca aactctgggg agcgcgggtg ctcaccaccg tcgggacgga cgccaaagcg
     1381 gccaaggcca aggcgctcgg ggccgacgag gtgatcaact acgccaaaaa ggatttcgcc
     1441 aaagaaacgc gcgccctcac cgacaaacgc ggcgtggaca tcgtggtgga gcacatcggg
     1501 caggacacgt tcgaaaaaag cgtcctctcc ctcgcccgga atggccgcct ggtcacctgc
     1561 ggctccactt ccggccggca gatccagttc gacctgcgca cttttttctc ccggaacctc
     1621 accatccacg gctcccgcat gggcacgcgg cggggattgg acgaagccgt ccgcttcctg
     1681 gccgccaagc ggctgagacc cgtggtggac aaggtgttcc ccctcaaaaa agccgccgaa
     1741 gcccacgtct acatggaaga acggaaaaac ttcggcaaaa tcgtcctgtc cgtctaagcc
     1801 gtccccaact gaaatctttt tatcccacaa ggaggcccat tcatgaatgt tgtcatcgcc
     1861 gtgacggtgg cgtcaattat ctggttcgtg ctggccggag ccctgttttt caatccgctc
     1921 gtcgaaccga tctacaacag cgaagagaac tcccccgccg ttaaaaaatt gccgaaaaca
     1981 ccgaaaacca tgggtctcat tatggcggcc atcctggccc agaccatcct ttgggccctg
     2041 gtctttaagt ggctgtcccc gcatttgtac tggccgcccc tctcaaaagg cctctttttc
     2101 ggcctcatgc tgacctgcat gaaaatggtg ccgcgtgacg tggaccgcct cctgctgacg
     2161 acgtatcccc tgaaacgcat ggctgtggaa ttcatcatcg gcaccgcgtg tagttttgtc
     2221 atcgggctca ctttcgcttt tatcatccgt tagacaaaaa cgctttcctt tccgtaaaaa
     2281 atcccggcgc ccgcgcggat gagcgcgtgc cttcaaaact tataatcgcc ctctccttct
     2341 cgcgttttgg taaaattaac gaagaaagga ggcgaccgcc atcatgccgt tgttcgaata
     2401 ttactgccga aaatgcaaaa aggatttcga ggttcttcac ctggccggac gggaaacgaa
     2461 ggccgcctgc ccctcctgtt cctccacgga catcatccag aaggtcagcg tgttcagtgc
     2521 acactccgcc gccccatcgg gaagctccgt tggccccgct aaccccggct gccggggctg
     2581 ccccaacgcc ggcggcgcct gcggactcga cggctgaccg gaatccctcc ggtttggccc
     2641 atagctccgt ctctcggcct cgcattcttt ttcgccgaat ccgaccgacg ggccttcgga
     2701 gccgcccgat tttttcggat tccggagttt ctctcccatg tctcttgacc tgacgcgcct
     2761 cacgcggccg ggggcgctct tcgagaaact cgacggcggc aacgtccgct gttttgcctg
     2821 cggccaccgc tgcctcatcg ccgacggacg ggaaggcgtc tgccgcgtcc gtttcaaccg
     2881 cggcggaacc ctttttgtcc cgcacggcta cgtctccgcc ctccacacgg atcccgtcga
     2941 aaaaaaacct ttttttcacg cccggcccgg cgccggagcg ttcagtttcg gcatggtggg
     3001 gtgtgatttc cactgcgact attgtcagaa ttgggaaata tcccagatct tccgcgaccc
     3061 cgcggccgtc ggaacggtcc gccccatgcc ggcggcggag ctgctccggg cggcgctgcg
     3121 gagcggggcg cagatcctgg ccagcaccta caacgaaccg ctgatcacct ccgaatgggc
     3181 ggcggagctg ttccgggaag ccaaaaaacg cggcctcttg accgcctatg tttccaacgg
     3241 caacgccacc ccggaagtgt tggagtatct caagccctgc ctggaccttt tcaaggtgga
     3301 cctgaagagt ttttccgagg accattaccg acgcctgggc ggcgacctgt cgaaagtgct
     3361 ggacagcctc cggcgcatcc acgagatggg cttctggctg gaggtcgtca ccttggtggt
     3421 gccgggcttc aacgatgccg acgacgaact gaaaaagatc gccgggcacc tcgccgccct
     3481 ctcgccggac atcccctggc acatcaccgc cttccatcag gactacaagc ggctctcctc
     3541 ggacaacacg cccgtggaga ccctggtgcg cgcggccaaa atcggccggc gcgaagggct
     3601 gcgttatgtg tatgcgggga acctgccgcg gatggtggga gactacgaga acacctactg
     3661 tccggactgc cgggaactgc tggtggaacg gcgggggttc gaggtgcgga aaaaccggct
     3721 ggccgccgac ggaaaatgcc cccaatgcgc cacggccatt ccgggccgct ggtaaaaaat
     3781 cagggacgcg gggccgggcc caacgtctct ttcaaccaac gggctatttt ttccttatac
     3841 gccgggtcgg atttctccca gtcccccatg ggatgcgggg cgccgggaag cggcagaaga
     3901 tcgcaagaaa ctccggccgc ccgaagtttc tcttgaaaac gaagcgattg ttcgaaagcc
     3961 acgctgcggt cccccgtccc atgcaccagg aggaacggtg gcagatcggg ccgcacgcgg
     4021 cggctcggcg aaatttcccg aagccccgca cgggcttttt catccaaccc gtcggaagac
     4081 agccccaaca aggcctggag cgcccgggaa actttccctc gacgctcgct gtccgcctcc
     4141 aagtccgtcg gcggcgccag ccccaccacc gcccccacgc gcgtctcctc cgtggagcga
     4201 accgccgcac agctcaccaa atgccccccg gccgagtagc cgatgagggc cacgcggcgc
     4261 gggtccccgc catattcggc ggcgtgcgcc tttacccacc gaacagcggt ctgcacatcg
     4321 tcgaaacagg cgggccagcg gtacttgggc gccaaccgat aattgacgga aaaacaggtg
     4381 aattccgccc ccaaaaactc cgccagcctc gcgatctcgc cctccttgtc ccccgtcgtc
     4441 cagccgcccc catggacaag cagcgccacc ggaaacgggc cggggccggc cggcccagag
     4501 acatccaaca agaggcggtc ttctccggcc tttccgtatt caatgtccgc ctgagagcga
     4561 cccgaaacag cggccagcgg acgcccggcc aaaaaaaata cgaggaggcc gacggttcgg
     4621 gcccttctcg tataaaaatg ttgttccctc ttcaaccatt ccattttcat agagtttaac
     4681 aaaattaatc cgtcgattcc ctgttcgagc gcccggggaa tcgtgctaaa attcatttca
     4741 ggatcgatac cccttcgtcc ggaaaggcct ggtaaaaaac gttgacccct ccccgccccc
     4801 ccaaaatact cgccgtggtc ggcagcccgc ggaaaaacgg caacacccat atcctcgttt
     4861 ctaaattatt ggaaagcgct tcaacggcgg gctcacaagc tgaaacggtt ttccttggcg
     4921 acctcaccct caaagaatgc gacggctgcc acgcctgctg gaaagggaaa ccctgcgcca
     4981 aaaaggacga catgctcggg ctctatccga agatc
//
LOCUS       DAOTFD010000194         5011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12107_length_5011_cov_4.312147, whole genome shotgun sequence.
ACCESSION   DAOTFD010000194 DAOTFD010000000
VERSION     DAOTFD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12107_length_5011_cov_4.312147"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4603)
                     /locus_tag="P5079_09530"
     CDS             complement(<1..4603)
                     /locus_tag="P5079_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY30260.1"
                     /translation="MPTLSKNDHRARLSRRPFWVRAVATATAGMFFCTQVVGATQVEK
                     SFWEQRRDAAQKKFTSDDTAGQDLPLELHRRRQGLSSEERQLLAQLPAAAAPLQFLPP
                     SSVSLPTRVTQGLPSPAPETPRDVPLSSLDLPDWITQVVLPYGHIRQIHFSPAPGAPL
                     VVHIQDAHEYADAQKNISSLIESLLSERGISLVGLEGAAGGFNLKPYRAFPDRKILRD
                     TADFLLRENLMGGAEHAGWVSEAEPQLWGVETAEPYFANIEAFRQALPSQPVLNDLLK
                     TWKNRIDGLKADVYSVQLRDFDQKLLDQREGSLSLGKYVRYLTLAGTPATGRHPQLDS
                     FVKALELEETLDFDQVEKERVQLIELLSQRLPEDSLDRLIEKSLLYRLGRLSYGDYHR
                     YLKTLCSSNGIDLSQFVFMGRYIEYVLTAEGISRDKLFAEVSDVENLILARLLVTPEQ
                     KELRAISDHYLLIKKLAEFVVAPEDWLYFSKNRAEVLALPRRLNALLKKTGTDTADDD
                     PDLTTSLRPFEDFCSLAVQRNDALLDNLLGKMNSQKQRAAILVAGGFHTQGLTDLMRR
                     KNVSYVVVTPKFQAVDKNAHYLDFLARDKRPLEKLLSGEKITLKSPIVGTGALWNLAE
                     RGFDGRWKVRLTAATAAMLWMAAHPDGRLPPEIRETLLALEGVDGLDVSTPETQAFIS
                     SIEVRLNKTIGLRFTATQKANEAAFKKSAEYSKVKSRRVVAEGVIGDYYVVLTSQRPG
                     LWTRARGWVSQRAAALGAWVRRLQKKIAELKAAERKSALRVVPDLTVAALGLPNKVCQ
                     DAHFSGRVDLKVPNGRGTLLCVFDGQGANGHHVAQTARDNVDKIFKRFLLDSKGDVAA
                     ALQRTAKGLVDWIGNRADGAATLSIVYVPDGAATGYAVCLGDSPVLWSPSGDGRPKGI
                     THPGVQSAGVHFGNSYRFSGKLPHQHLLGSESTAGPLELDSFTFDLPPDGVVMVASDG
                     FADLPELTEKGLVDYARRVEPWAKAGVPADEIVRRARKTKILESNDDVTLLVLRPPQE
                     PSLIGALWSRTTAGRWTTPRLARFSLIVRSTLKYVFGYDLTAPRDPSERRFGSVWPVR
                     FALVTTTDPQGITRHALSLGAFGFRKDIALTPAGKGVLHTVLVELVSFLFLWEGLRGS
                     FWMVLENPPLILSMATGILLSAAATAYFVLWIFQGFRELLRPAQDHSKTWERAVRRTS
                     RVLSFVLPPLLLLSGSILFPNRLGLAVAAEGLGLAGLLQGWPLTRLGARLTAALRPQA
                     LFSRLANHLPGRAARVAAQRGLLEKSIEGRLQTIYGDKAVSLRPKLTALLNDFRRQHA
                     ALAGDIPYGERLSERDAVLVSYGDSIQGARAGAHLAALRRFLRKHLRGAVNAVHFLPL
                     NRSTSDRGFAPVKHAEVDPRYGTQEDIADIQSDGFDAMHDFVVNHVSASNERFQAFLN
                     GDAGARRYFHVYDRESDLPAEARALVFRPRTTPLLTPFTVRDPATGKTRTVFVWTTFS
                     ADQIDLNLENEEAFLETVRALLDYAAQGARYIRLDAVGYL"
     gene            4809..>5011
                     /locus_tag="P5079_09535"
     CDS             4809..>5011
                     /locus_tag="P5079_09535"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRY30261.1"
                     /translation="MVTFSYMDSLTIHGLTLRVRVGCTARERARPQRLSADIRLETSL
                     RRAGERDDLRFTVDYAALAGRLRT"
BASE COUNT          887 a         1446 c         1779 g          899 t
ORIGIN      
        1 agagatagcc cacggcgtcc agtcggatgt agcgcgctcc ctgggcggcg tagtccagga
       61 gggcccgaac ggtttccagg aacgcctcct cgttttccag attgaggtcg atctggtcgg
      121 cgctgaaggt ggtccagacg aagacggtgc gggttttccc cgtggccggg tcgcgcacgg
      181 tgaacggcgt caggaggggg gtggtcctgg gacggaacac gagggcacgg gcctcggccg
      241 gcaggtcgga ctcccgatcg taaacgtgaa aataacgccg cgcaccggcg tcgccgttca
      301 ggaacgcctg gaaacgctcg ttggaggcgg aaacgtggtt gaccacgaaa tcgtgcatgg
      361 cgtcaaaacc gtcactttgg atgtccgcga tgtcttcctg ggtgccgtag cgcggatcca
      421 cctcggcgtg cttgaccggc gcgaacccgc ggtccgaggt ggatcggttc agaggaagga
      481 agtggacggc gttcaccgcg ccccgcagat gtttccggag gaaccggcgc agcgccgcca
      541 aatgcgcgcc ggcccgcgcc ccctgaatgg aatcgccgta ggaaacgagg acggcgtccc
      601 gttcgctgag gcgttcgccg taggggatgt ctccggccag cgcggcgtgc tgtcggcgga
      661 aatcattcag caacgccgtc agtttcggcc gcagagaaac ggctttgtcc ccatagatcg
      721 tttgaagccg cccctcgatg cttttttcga gaaggccccg ttgtgcggcg acccgtgccg
      781 cccggcccgg aagatggttg gccaggcggg agaagagggc ttggggtcgg agcgcggcgg
      841 tgaggcgagc tcccagacgc gtcagcggcc acccttgcag gaggcccgcc aaccccagtc
      901 cttcggccgc cacggccagt cccagccgat tgggaaacag gatggagccc gacaacagga
      961 gaaggggcgg gagaacgaaa gagaggacac ggctggtccg gcgaacggcc cgctcccagg
     1021 ttttggaatg gtcctgggcc ggccggagga gttcgcggaa cccttgaaaa atccaaagga
     1081 cgaaataggc ggtcgccgcc gccgacagca atatccccgt cgccatcgag aggatcaggg
     1141 ggggattttc caagaccatc cagaagcttc cccgcagtcc ttcccacagg aagaggaaag
     1201 aaacgagttc cactaaaacg gtgtggagaa cgcctttccc ggccggggtc agggcgatgt
     1261 ctttgcggaa tccgaacgcg ccgagggaga gcgcgtggcg cgtgatcccc tggggatcgg
     1321 tggttgtgac caatgcgaat cgcacgggcc agaccgaacc gaaccggcgc tccgaaggat
     1381 cgcgcggcgc ggtgaggtcg tagccgaaca cgtatttcag agtgctccgg acgatgaggg
     1441 agaatcgggc gagtcggggc gtggtccacc ggccggccgt cgtccgcgac cacaaggccc
     1501 cgatcaggga cggctcttgg ggcgggcgaa ggaccagcag ggtgacgtcg tcgttggatt
     1561 cgaggatttt tgttttgcgg gcgcggcgca cgatctcgtc ggccggcaca ccggctttgg
     1621 cccacggttc cacgcgccgg gcgtaatcga ccagaccttt ttcggtgagt tcggggaggt
     1681 cggcgaaacc gtcggaagcg accatgacga cgccgtccgg cggcaggtca aacgtgaaac
     1741 tgtcgagttc gagcggcccc gcggtgctct ccgagccgag cagatgttgg tgcgggagtt
     1801 tccccgagaa acggtaggaa ttgccgaaat ggacgccggc gctctgcacg ccgggatgcg
     1861 tgattccttt cggtcgcccg tcgccgctgg gggaccagag gacgggggaa tcccccaggc
     1921 agacggcgta tcccgtggcg gcgccgtcgg gcacatagac gatggagagg gtggcggccc
     1981 cgtcggcgcg attgccgatc cagtccacca gtcccttggc cgtccgttgc agcgccgcgg
     2041 cgacgtcgcc ttttgaatcc agcaggaacc gcttgaaaat cttgtcgacg ttgtcccgcg
     2101 cggtttgggc gacgtgatgg ccgttggcgc cctgcccgtc gaacacgcag agcagggtgc
     2161 cccggccgtt gggaaccttc aggtccaccc ggccggagaa atgggcgtcc tggcagacct
     2221 tgttcggaag gcccagggcg gcgacggtga ggtcggggac gacgcggagg gcggatttcc
     2281 gttcggcggc tttgagttcg gcgatctttt tttgaagccg acggacccac gccccaaggg
     2341 ccgcggcgcg ttgggagacc caacctcgcg cacgcgtcca caggccaggc cgctggctcg
     2401 tcagcaccac gtagtagtcg ccgatgacgc cttcggcgac cacgcggcgc gacttgacct
     2461 tggaatattc cgcgctcttc ttgaacgccg cctcgttggc tttctgggtg gcggtgaaac
     2521 ggaggccgat ggttttgttg aggcgcacct cgatggacga gatgaacgcc tgggtctccg
     2581 gcgtggagac gtcgagcccg tccaccccct ccagggccag gagcgtttcg cggatctccg
     2641 gcggcaaacg gccgtcgggg tgggcggcca tccagagcat ggcggcggtg gcggcggtga
     2701 ggcgcacctt ccagcggccg tcgaagccgc gttccgccag gttccacagg gcgccggtgc
     2761 ccacgatggg ggattttagc gtgatctttt cgccggaaag gagtttctcc aggggccgtt
     2821 tgtcgcgggc gagaaaatcg aggtaatgcg cgttcttgtc gacggcctgg aacttgggcg
     2881 tcaccaccac gtaggaaacg ttcttgcggc gcatcaggtc ggtgagcccc tgcgtatgga
     2941 acccgcccgc caccaggatg gccgcccgct gtttttggga attcatcttt cccaacaggt
     3001 tgtccaacag ggcgtcgttc cgttggaccg ccagggaaca aaaatcttcg aaaggacgga
     3061 gggaggtcgt caggtcgggg tcgtcgtccg ctgtgtcggt gccggttttt ttcaacaggg
     3121 cgttgagtcg ccggggcagg gcgaggacct cggcccggtt cttcgagaag tagagccagt
     3181 cctccggcgc cacgacgaat tcggcgagtt tcttgatcag gaggtaatgg tccgagatcg
     3241 cccggagttc cttttgttcg ggggtgacga gcagccgcgc caggatgagg ttctccacgt
     3301 ccgacacctc ggcgaaaagt ttgtcgcgcg agatgccttc ggcggtcagc acatactcga
     3361 tgtagcggcc catgaagacg aactgagaga gatcgatgcc gttggaggag cagagggtct
     3421 tcaggtagcg gtggtaatcg ccatagctga gccggccgag acggtagagg agacttttct
     3481 caatgagacg gtcgagcgag tcttccggca ggcgttggga gaggagttcg atcagctgga
     3541 cccgttcttt ttccacctgg tcgaaatcca gggtctcttc caattcaagc gctttaacga
     3601 aggagtccag ttgcgggtgt cggcccgtcg ccggggtccc ggccagggta agatagcgga
     3661 catattttcc gagggagagg gagccttccc gctggtcgag gagcttctgg tcgaaatccc
     3721 ggagctggac ggaatagacg tccgccttca gcccgtcgat ccggttcttc caggttttca
     3781 ggaggtcgtt gaggacgggt tgggagggaa gggcctgccg gaaggcctcg atgttggcga
     3841 aatacggttc ggcggtctcc accccccaga gctggggttc cgcttccgag acccatccgg
     3901 cgtgctcggc gccgcccatg aggttctccc gcaggaggaa gtcggcggta tcccgcagga
     3961 tcttgcggtc cgggaaagcg cggtagggtt tgaggttgaa cccgccggcg gccccttcca
     4021 gtcccaccag cgagatgccg cgttccgaca gcagggactc gatgagcgag gagatgttct
     4081 tctgggcgtc ggcgtactca tgggcgtcct ggatgtggac cacgaggggc gcgcccggcg
     4141 ccggggagaa gtggatctgg cggatgtggc cgtacgggag gaccacctgg gtgatccagt
     4201 ccggtagatc gagggaggag agagggacgt cccgcggggt ctccggagcg ggggacggga
     4261 gaccttgcgt gacgcgggtg gggagggaga cggaggaggg aggcaggaac tggagcgggg
     4321 cggcggcggc cggcagctgg gccaggagtt gacgttcctc ggaggagagt ccctggcggc
     4381 ggcggtggag ttcgaggggg agatcctgtc cggcggtgtc gtcggaggtg aacttcttct
     4441 gggcggcgtc gcggcgctgt tcccagaaac tcttctccac ctgggtggcg cccaccacct
     4501 gggtgcaaaa gaacatcccc gccgtcgcgg tggcgacggc gcggacccaa aacggtcggc
     4561 ggctcaggcg agcgcggtga tcgtttttgc tgagcgtcgg caaggatctc tccctcggca
     4621 aaatgccgaa caactccttc gtgccttcat cggcccgaag tttgcgtttt taatgtctat
     4681 gtgatgcggg tgctgcgaac tagagacgaa gtctccctca cccccttttt acccgaaaaa
     4741 aagccctatt tcaaggggtt ctataaaata gtttctacta gacacaaatt gacacctctg
     4801 gacacaaatt ggtaactttt tcttatatgg acagcctgac cattcatgga ttgacgttgc
     4861 gggtgcgggt gggttgcacc gcccgggagc gagcccggcc gcagaggctc tccgcggaca
     4921 tccggctgga gacctctctt cgccgggccg gcgagaggga cgacctgcgg ttcacggtgg
     4981 attatgccgc gctggccggc cggctccgga c
//
LOCUS       DAOTFD010000195         4949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12312_length_4949_cov_4.490805, whole genome shotgun sequence.
ACCESSION   DAOTFD010000195 DAOTFD010000000
VERSION     DAOTFD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12312_length_4949_cov_4.490805"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4949)
                     /locus_tag="P5079_09540"
     CDS             complement(<1..>4949)
                     /locus_tag="P5079_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30262.1"
                     /translation="VSLNIPFDVIGKAARGEMRTDDRYRQKGDDLLRAGRVRNLLMAG
                     GSASRWKCSLETLIKAGLVDEAEVPESLPRALAAYWLGSYTDPDDSYTGVGLAIDNAV
                     SQEQNGYLAPFGYVLSPYTWVDFMKETWKRIDRADEPIEVAAISQNSGEALVVQDGKM
                     TGERLPGKVLGHGNVRGWILGVIEGLKKGVVFDIAHSADNPFSQPPMTQFREIVGRMS
                     EDVLVHVALGNWRNIGQFGGGFVNVNGTPNVIDTAIAKEENKDGFSANRDINIFSLFI
                     NVVNNAALLYAVSDGMVVLDEAYREQPLISWEEVQALTRGDDAAVWQRVSSRLERLTE
                     AAQAQLMDNFISLLPSEYVTKSEGGKDFVQWEQISGMLHGAMVRAIQARHAKEGVEWR
                     RPLNMAYVDISLNDMGILYGRTPDQAAIGEYTPTFAEVKSASDLYTVLDRVRFLLGVM
                     KSRKMFSDAVKLGSTAADLAAQTAAEAGKLVKTVAADAAPQPLVDILSLLAPREVEDS
                     ARWFERQEQTAGNIVMEQIMADSEAVIKEFWRMVQPGKMISAEDLDAFFVFLAKRLRE
                     NAPALQKAMGDVRIKGLSGAPQFDFANIRIERSPADRFSYKLILPIIKTPDITDLPDA
                     EFLEINVVDVLEIGQEEATRKEASLEIIHRFQSPLSKNSFVAITISLVTRTDLTFRGV
                     AIRGWPLRVINWLTSTRLYIWLSYRLMARASRKTQATSPTEGAKPPAGAGPIGNLHVG
                     TVRELVQFFASRVPFAKVAKTVAAELEISQKTAKELLQALAGGVSDGIVREFRGAMPR
                     LLSIYLDAAWKKAAAKGDQKLGAEISGLVKKGVATPGFEGLGLKGKPVQVVVMDGEFG
                     TGVDLYALARFQIQEGKPAQFQIHRKLVDYIQKMDGKKRAEFLQTLVVRELTLQLLLS
                     QGSTYDKASQEILQSQEQFDLLKEVNLNVVFPAQGLTLELMRRYAGQRVSVAYDALPE
                     RGGRGTVVFRMPSSPEEAVGYSKDSARPFVKVFVEEHDWDKEKGGPAEPSQDLTLRLD
                     RFDMQTVKGLDQEVAAKVEAPAEAPTAKVEKPASPVAPAEIVFASNGDDWEGLYVDGQ
                     LVSEGHSLRPGEVLEALKLDFAELDDDGIMAYLEDAGNFPENLGELTKKKITNKIVLV
                     HGDDWNGFYIDGKLAMEGHSYDSRHVLDALKIKSTRLPEKVVNKGLDRFGGRLPEKLS
                     DMVKKEEKKPAKVASAPKTKTSESVELSPEQKDFENYLNFLRFVVSELSAMPLDEMKV
                     GVLYDHPVNGPELKKALGKIVTALGKFEKAGRISEEDDEKLRQAVRSSFKLPRSMEYY
                     DVDGDIMLEEGDSRAAILVKLLTEYALVRNHPSEIAEIYKVDISKARPVPSVAKTATV
                     PTIKSAEGGVVVEVHTDGHVYVVGDASGKRVQFPRDLRIPGARFRVAGVVDAGSFYRL
                     AKGGKPQQISGLTAGAWHEGDMAELLTEIASANAPQLIAALQSAAEMTLTLTSAGLNQ
                     WASRLMDLAEELQFGISREEFLADVADLPDGVRMAVLHALAVDQTVRQDWTDDQRKNL
                     ETVLRAAGAEVSLVEATPQVLLYDVTREGRTQRVRVYLSKTPSLLQREGLHALATIRG
                     PTAADVVAEISFQAALFEMEAARPLEIVARHELDELFDI"
BASE COUNT          927 a         1539 c         1402 g         1081 t
ORIGIN      
        1 caatatcaaa gagttcgtcc agttcgtgcc gagcgacgat ctccagcgga cgggcggcct
       61 ccatctcgaa caaggcggcc tggaaggaaa tctcggcgac cacgtccgcc gccgtggggc
      121 cgcgaatggt ggccagggcg tgcagccctt cccgttgaag gagggacggc gtcttcgaga
      181 gatagacgcg gacccgctgg gttcggccct cgcgggtgac atcgtagaga aggacttgcg
      241 gagtggcctc caccaggctc acctcggccc cggcggcgcg taaaacggtt tccagattct
      301 tgcgctggtc gtccgtccag tcttggcgga ccgtctggtc cacggccagg gcgtggagga
      361 ccgccatgcg cacgccgtcc ggcagatcgg ccacgtcggc gaggaactcc tcgcgggaaa
      421 tgccgaactg cagttcctcg gccaggtcca tcaggcggga ggcccattgg ttcaacccgg
      481 cgctggtgag ggtgagggtc atttccgccg ccgactggag ggcggcgatc agttgcggcg
      541 cgttggcgga ggcgatttcg gtcagcagtt cggccatgtc gccctcatgc caggccccgg
      601 cggtgaggcc tgaaatctgt tggggtttgc cgcccttggc aagccgatag aaacttccgg
      661 cgtcgaccac tccggccact ctaaatcggg cgccgggaat gcggaggtct ctggggaact
      721 gaacgcgttt gccgctggca tcgcccacga catacacatg gccgtcggta tggacttcca
      781 cgacaacccc gccttcggcc gatttaatcg tcggaaccgt cgcagttttt gcgacggatg
      841 gaacgggccg ggctttgctg atatccacct tatagatctc ggcgatttcg ctgggatgat
      901 tccggacaag ggcatactcc gtcaaaagct tcaccaggat ggcggcgcgg gaatcacctt
      961 cctccagcat gatatcgccg tccacatcgt aatactccat cgagcgggga agcttgaacg
     1021 aagaccgaac cgcctgtctt aacttctcat catcttcttc cgatatacgt cccgcctttt
     1081 caaattttcc caacgcagtc acaattttac cgagcgcctt tttaagttca gggccgttga
     1141 ccgggtggtc atacaacacg cccaccttca tttcatcgag cggcatcgcc gaaagctcgc
     1201 tcacgacaaa acgaaggaaa ttgaggtagt tctcaaaatc tttctgttcg ggcgacaact
     1261 cgacggactc agacgttttt gtttttggcg ccgaagcgac cttggcgggc ttcttttctt
     1321 cttttttaac catgtccgac aacttttcgg gcaaccgtcc gccgaaccga tccaacccct
     1381 tgttcacgac tttttccggc agccttgtgg attttatttt cagcgcatcc aagacatgcc
     1441 tcgagtcgta ggagtgcccc tccatggcca atttaccgtc gatatagaac ccgttccaat
     1501 cgtccccgtg gacgaggacg atcttgttgg ttattttttt cttcgtcaac tcaccgaggt
     1561 tttccgggaa attgccggcg tcttccagat aggccataat gccgtcgtca tcgagttccg
     1621 cgaaatccaa tttcaacgct tccagaactt cccccggccg caaggaatgt ccttccgaga
     1681 ccagttgtcc gtcgacgtag agcccttccc agtcgtcgcc gttcgaggcg aacacaatct
     1741 cggccggcgc gacaggtgac gccggttttt caaccttcgc cgtcggcgct tccgcggggg
     1801 cctcgacttt cgccgcgact tcctgatcca accccttcac ggtttgcatg tcgaaccggt
     1861 cgagccggag cgtcaggtct tgggagggtt ccgccgggcc gcccttctcc ttgtcccaat
     1921 cgtgctcttc gacgaagacc ttcacgaacg gcctcgccga gtctttggaa taccccacgg
     1981 cttcttccgg ggaagacggc atgcggaaca ccaccgtgcc ccgtccgccc cgttccggca
     2041 gtgcgtcgta ggccaccgac acccgctggc cggcgtatcg acgcatcaat tccagcgtga
     2101 ggccctgggc cgggaacacc acgttgaggt tcacttcttt caaaaggtcg aattgttctt
     2161 gggattgcag gatttcctga gaggccttgt cgtaagtgga gccttgggag agcaaaagct
     2221 ggagggtcaa ttcccgcaca acgagcgtct ggaggaattc cgcccgtttt ttgccgtcca
     2281 tcttctggat ataatccacc agtttccgat ggatctggaa ctgcgccggt ttcccctcct
     2341 ggatttggaa ccgcgccaga gcgtacaggt cgacgcctgt gccgaactcg ccgtccatca
     2401 cgaccacctg gaccggcttg cctttcagtc cgagcccctc aaaccccggc gtggccacgc
     2461 ctttcttgac gaggccggag atttcagcgc cgagtttttg atcgcctttg gccgcggcct
     2521 ttttccatgc cgcgtccaga tagattgaca aaaggcgggg catggcgccg cggaactccc
     2581 ggacgatccc gtcggaaact cccccggcca aagcctggag taattctttg gcggttttct
     2641 gggatatttc cagttccgcc gccacggttt tggcgacctt cgcaaacggc acgcgacttg
     2701 cgaagaattg caccagctcc cgcaccgtgc ccacgtgaag attcccgatt ggcccggctc
     2761 cggcgggcgg cttggccccc tctgtcggag atgtcgcttg agttttacgc gatgcccggg
     2821 ccatgagtcg ataagagagc caaatataga ggcgtgtgct cgtcagccaa ttgatcactc
     2881 tcaacggcca ccctcttatg gccacccctc taaaggtcaa gtccgtccga gtaaccaagg
     2941 aaatggtgat ggccacaaat gaattcttgc tgagaggcga ctggaaacgg tgaatgatct
     3001 ccaaagaggc ctctttgcga gtcgcctcct cctgtccaat ttcaagcaca tccacgacgt
     3061 tgatttccag aaactccgcg tccggcagat cggtgatatc gggtgttttg attataggga
     3121 ggatgagttt gtaactgaaa cgatcggcgg gggaccgttc tatgcggata ttggcaaaat
     3181 cgaactgggg cgcgcccgac aatcctttta tgcgcacgtc tcccatcgcc ttctgaagcg
     3241 ccggggcgtt ctcccgcagt ctcttcgcca ggaaaacgaa gaacgcgtcc aagtcttcgg
     3301 cggaaatcat tttccccggt tgcaccatgc gccaaaattc ttttatgacg gcctctgaat
     3361 ccgccatgat ctgttccatg acgatgttcc ccgcggtttg ttcttggcgt tcaaaccatc
     3421 tcgccgaatc ttcgacctcc cggggggcca ggagcgagag aatatcaacg agaggctgtg
     3481 gggccgcatc ggcggcgacc gtcttgacca atttcccggc ttcggccgct gtctgagctg
     3541 ccaaatccgc agcggtcgaa cccaatttga cggcatcgct gaacattttg cggcttttca
     3601 tgacgcccaa gaggaagcgg acccggtcca gaaccgtgta gaggtccgag gcggatttga
     3661 cttcggcgaa ggtcggcgtg tattccccga tggcggcctg gtcgggcgtg cggccgtaga
     3721 ggatccccat atcgttcaag ctgatgtcca cataggccat gttcaacggc cggcgccatt
     3781 caacgccctc tttggcgtgc cgcgcctgga tggcccgcac catggccccg tgcagcatgc
     3841 cgctgatctg ctcccactgg acgaaatcct tgccgccttc gctcttggtc acgtattcgg
     3901 acgggaggag gctgatgaag ttgtccatca attgcgcctg ggccgcttcg gtcagacgtt
     3961 ccaaccggga actcacccgt tgccacaccg ccgcgtcgtc tccccgagta agcgcctgca
     4021 cctcttccca ggaaatcaga ggttgttcgc gatacgcctc gtccaggacg accatgccgt
     4081 cactcacggc gtaaaggagc gcggcattgt tgacgacgtt gatgaagagc gagaaaatgt
     4141 tgatgtcgcg attggcggag aatccgtctt tgttctcctc cttggcgatg gcggtgtcga
     4201 tcacgttggg agtgccgttg acgttcacga acccgccgcc gaattggccg atgttgcgcc
     4261 agttgccgag ggccacgtga acaagcacat cctcggacat acggcccacg atttcacgga
     4321 actgcgtcat gggcggctgg gagaaggggt tgtccgccga atgggcgatg tcgaacacca
     4381 cgcctttctt caggccttcg atgaccccca aaatccagcc gcggacgttg ccgtgcccca
     4441 aaacttttcc gggaagacgt tcccccgtca tcttgccgtc ctgaacgacc agcgcttcgc
     4501 cgctgttctg ggagatggcg gcgacctcga tgggttcgtc ggcgcgatcg atgcgtttcc
     4561 aggtttcctt catgaagtcg acccaggtgt acgggctcaa aacgtatccg aacggcgcca
     4621 agtatccgtt ctgttcctgg ctcaccgcgt tgtcgatcgc cagtcccacg ccggtataac
     4681 tgtcgtcggg gtcggtgtaa ctgcccagcc aataggcggc cagggcccgc ggcaggcttt
     4741 ccggcacctc ggcctcatcc accaaacccg ccttaatgag ggtctccagg gaacacttcc
     4801 agcgggacgc cgacccgccg gccatcagaa ggttccgcac ccgtcccgcc ctcaaaagat
     4861 cgtcgccttt ttgccgatag cggtcgtccg tgcgcatctc gccgcgcgcg gctttcccga
     4921 tgacgtcgaa cgggatgttc agcgacacc
//
LOCUS       DAOTFD010000196         4932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12372_length_4932_cov_3.277425, whole genome shotgun sequence.
ACCESSION   DAOTFD010000196 DAOTFD010000000
VERSION     DAOTFD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_4932_cov_3.277425"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4932
                     /locus_tag="P5079_09545"
     CDS             <1..>4932
                     /locus_tag="P5079_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30263.1"
                     /translation="KAIKAGDFTAIEKFFARATGLRVESVKSFTDHGQSGIRVEFAQG
                     GRLSIVQTVNGATVKAEKVGKAGDGRSRAMSKKISQAGARWVRAQRPPLGWKTVFIVF
                     TAFTLILGVSLAFWLTPLYYPNIAWTILDAVSMIARYARIFFPIPMIYFEFMMILLVS
                     SGIGFTTTTLLRRALVRRMDRAEIPKHRYGLAPIISRNRQVNMKDMLPKPMNSWAFFL
                     KRPNGTLEVYRGVELLPPVVQVFFYARDMIVASYNIKVKFMPRFVSGILIGLVRGALG
                     PILNTPRYVWILLRYLVESLLPYRAPPAVAGTAISMDQLRGVYDVSAGGSFHIVTGII
                     TVVPDGDGIKVRLPDGQIITIRFMRYNSFGNPDPHAGPLSSAEVQKDIARYAGKLTPQ
                     QVIDAGRKGKEEAVRLLLPGTPVILLLHYRNGKPVLAVGGRHVADGYIIDPDRPDRRV
                     LITDKLKERMGGPDAENVTWDNPYAHMSADAQTLPSYIQGDALEGPAGQRLITVSNRI
                     TPWLWRFWFVANVAALIYLLPLLPAGYPILKILLFSAAWFNFGIGHYYGGAVLKLVSA
                     IWNALKAIGRRFSTSARPVVQAGLPAAAVSAATPNAAMSPTDIEDLYNRTIEVLKTGR
                     GWDQNDLAGTVRTLVGWDQLLPLYRRLGVNPSEILEAWMERHQAGVNVFGISKPNTQI
                     NSPVRLPENVEGTLLRQGSYNSLDDKIKRLLERRVAAMLGLKKKPGRGETLEIGTLSG
                     AQIKAYLDGGETGDPAFKLLMQDKTREQWLWRMAIEEGWADDMIKAMTLLAGFVSQMR
                     KQFHDTQTLVISPLSLFEVIDWAKATHMGEKPENWKGPLNLRLMLIDEATQQGFQKTN
                     VPAARRAPQQVDPRTIPDAVGFFGTRVNVSATRVGGEWLRNTTNSDEVNPLRYEFLHG
                     ITAFGGLMKQLRHHGDWHVRDDLHLMAASFFRSLGTSRGVDLGTRLVRFVLRHTGSSS
                     VATDVGTEWGRQISQEDAGLARDLAARIQIRNLFARAALTHTPSRGAPPVGGILWGKT
                     VGADEALAAVERTSFLSEDRGRTQLLGVLRQALSAEQTSHDDVFILPWGETHNRIEVR
                     VRLAQRAPPLPGDSSDLFRWGSILRAGPMSQAAHVDRYDLILNTELLKLTDQQLAAVV
                     QTYFARIQQLSQGSSFETVRKKPIVDSEGRILPSLRELSQGLELRRFQGYLPQWVEMD
                     ADGWLNVASQEDVLRAAQGMLTYWKMETPDESRPILIGYSTEAHRDLAAQVARLAAAN
                     GRRVLLASQPGSVNSLQQSPQDAAGRFYIGNGAVRFLGAEGSVFDKTYLVPDKLNLAV
                     SLTREYLTTDAQGHLIDSVDLPEAAADAPRVEKPKGKEARAYPAQDNTRLPGLFTWTN
                     LIYATVFLGLLILAATIFIWNPAVHLQITGLPWLSRITRYGTFFMIRGITAVITFIVN
                     YFFMAFVGILQYVFFYLLTQRKLIRQMDEKQDIAPIDLPPEIARFPEFNITEDPSTLK
                     EKPLVKLGENGRVYVNHRALGIAYAMFQQSRWVHLIHTATFGLVKKETLGRMRLLLFF
                     ESALAQAKQDSFLAPIQQIPLLGMFTRRGGRYSWLIQGILWQPFLIVRGFFRSIKMWF
                     VPEAAALGESLAYTSLPTDLAQKGFTLHYGCVR"
BASE COUNT          900 a         1759 c         1438 g          835 t
ORIGIN      
        1 tcaaggccat caaggccggc gatttcacgg ccatcgagaa attctttgcg cgcgccacgg
       61 gcctgcgggt ggaaagcgtc aaatccttca ccgaccacgg tcagtccggc atccgcgtgg
      121 agttcgcgca aggcggacgt ctttccatcg tgcaaaccgt caacggcgcc accgtcaagg
      181 cggaaaaggt cggaaaagcg ggcgacggcc gttcgcgggc gatgtccaaa aagatatccc
      241 aggcgggcgc ccggtgggtc cgggcccagc ggccgccgtt gggatggaaa accgtcttca
      301 tcgtcttcac ggcgttcacc ctcatcctgg gcgtgtcgct ggcgttctgg ctgaccccgc
      361 tttactatcc caatatcgcc tggaccatcc tggatgcggt ttccatgatc gcccgttacg
      421 cccgcatctt cttccccatc cccatgattt acttcgaatt catgatgatc ctcctggtgt
      481 cgtcgggcat cggcttcacg accaccaccc tcctgcggcg ggcgctggtc cgccgcatgg
      541 accgcgcgga gatccccaaa caccgttacg gcctggcccc catcatctcc cgcaaccgcc
      601 aggtgaacat gaaggacatg cttcccaagc cgatgaacag ctgggccttc ttcctgaaac
      661 ggcccaacgg cactctggaa gtctaccggg gcgtggaact tctgccgccg gtggtgcagg
      721 tgttcttcta cgcccgggac atgatcgtgg ccagctacaa catcaaggtt aaattcatgc
      781 cccgcttcgt cagcgggata ctgatcggcc tggtgcgagg cgccctcggc cccatcctca
      841 acaccccgcg ttacgtctgg atcctcctcc gctacctggt ggagtcgctc ctgccgtacc
      901 gcgcgccgcc cgcggtggcc ggcaccgcca tctccatgga ccaactgcgc ggcgtctacg
      961 atgtttccgc cggaggcagt ttccacatcg taacggggat catcactgtt gttcccgatg
     1021 gcgacggcat caaggtccgc ctgcccgacg gccagatcat caccatccgc ttcatgcggt
     1081 acaactcttt cggcaacccc gacccccacg ccggcccgct ctccagcgcc gaagtccaga
     1141 aagacatcgc tcgttacgcc ggcaaactca cgccgcagca ggtcatcgac gccggccgga
     1201 aagggaaaga ggaggccgtc cggcttctgc tccccggcac ccccgtcatc ctcctcctgc
     1261 attaccgtaa cggcaaaccg gtcctggcgg tgggcggacg ccacgtggcc gacgggtaca
     1321 tcatcgatcc cgaccggccc gaccggcgcg tgctcatcac ggacaaattg aaagagcgca
     1381 tgggcggccc cgacgccgag aacgtcacct gggacaaccc ctacgcccac atgagcgcgg
     1441 acgcccagac gctcccatcc tacatccagg gggatgccct cgaaggaccc gcgggccaac
     1501 gcctgatcac cgtctcgaac cggatcactc cctggttgtg gcgtttctgg ttcgtggcca
     1561 acgtggcggc gctcatctat ctgttgcccc tgctcccagc gggctatccg atcctcaaaa
     1621 ttctcctctt ctccgccgcc tggttcaact tcggcatcgg ccattattac ggcggagcgg
     1681 tcctgaaact cgtttcagcg atatggaacg cgctgaaggc catcggccgg aggttctcca
     1741 catcggcccg cccggtggtc caggccggtc ttcccgccgc cgccgtctcc gccgccaccc
     1801 ccaacgcggc gatgtccccg acggacatcg aagacctcta caaccgcacc atcgaggtgc
     1861 tcaaaacggg acgcggctgg gaccagaacg atctggcggg cacggtccgg accctggtcg
     1921 gttgggacca gttgcttccc ctctaccggc gtctgggcgt gaatccgtcg gaaatcctcg
     1981 aagcgtggat ggaacggcac caggccggcg tcaacgtctt cggcatctcc aaacccaaca
     2041 cgcagatcaa ctccccggtg cgtctgccgg agaacgtgga aggcacgttg ctgcgacagg
     2101 gttcctacaa ctcgctcgac gacaagatca agcggctcct ggaacggcgc gtggcggcga
     2161 tgctcgggct gaagaaaaaa cccggccgcg gcgagaccct cgagatcggc accctgagcg
     2221 gcgcgcagat caaggcctat ctggacggcg gcgagaccgg cgacccggcc ttcaaactat
     2281 tgatgcagga caagacccgc gaacagtggc tgtggcgcat ggccatcgaa gaggggtggg
     2341 ccgacgacat gatcaaggcc atgaccctgc tcgccgggtt cgtgtcgcag atgcggaaac
     2401 aattccacga cacgcagacc ctggtcatca gcccgctctc ccttttcgag gtcatcgact
     2461 gggccaaggc cacccacatg ggcgagaagc ccgagaactg gaagggaccg ctcaacctgc
     2521 ggctgatgct catcgatgaa gcgacccagc agggtttcca gaaaaccaac gtgccggcgg
     2581 cccggcgggc cccgcagcag gtcgacccgc ggaccatccc cgacgcggtc ggtttcttcg
     2641 gaacccgggt gaacgtctcc gccacccgcg tgggcggcga atggctgcgc aacacgacga
     2701 attccgacga ggtgaacccg ctccgctacg aattcctgca cggcatcacc gccttcggcg
     2761 ggctgatgaa acaactccgg catcacggcg actggcacgt gcgcgacgac ctgcatctga
     2821 tggccgcctc gttcttccgg tccctcggga cgtcccgggg cgtcgatctg ggcacgcgcc
     2881 tggtccgctt cgtcctgcgg cacaccggca gcagcagcgt ggccaccgac gtggggaccg
     2941 agtggggccg gcaaatctcc caggaagacg ccgggctggc ccgcgacctg gcggcccgca
     3001 tccagatacg caacctcttc gcgcgggcgg cgctgaccca caccccgtcg cggggcgccc
     3061 cgccggtcgg cggcatcctc tggggcaaaa cggtgggcgc cgacgaggcc ctggcggccg
     3121 ttgagcgaac gagtttcctc agcgaggacc gcggccggac acagctcctc ggcgtgttgc
     3181 ggcaggccct gtcggcggaa cagacctcgc acgacgacgt cttcatcctg ccgtgggggg
     3241 agacccacaa ccgcatcgag gtgcgggtcc ggctggccca gcgcgcgccg ccgctgccgg
     3301 gcgactcctc cgaccttttc cggtggggct ccatcctccg ggccggaccc atgtcgcagg
     3361 ccgcccacgt ggaccgttac gatctgatcc ttaacaccga actcctcaaa ctcaccgacc
     3421 agcagttggc cgccgtggtc cagacttact tcgcccgcat acaacagctc tcccaaggct
     3481 cctcttttga aaccgtccgg aagaaaccga tcgtggacag cgagggccgg atccttcctt
     3541 ccctgcggga actttcccaa ggcctcgagc tgcgccgctt ccagggatac ctgccgcaat
     3601 gggtggagat ggacgccgac ggctggctga acgtggcctc ccaggaagac gtgctccgcg
     3661 ccgcgcaggg catgctcacc tattggaaga tggaaacgcc cgacgaatcc cggcccatcc
     3721 tcatcgggta cagcacggaa gcgcaccggg acctggccgc ccaggtggcc cgtctcgcgg
     3781 cggccaacgg acgccgcgtg ctgttggcct cccaacccgg cagcgtgaac agtctccaac
     3841 agtccccgca ggacgcggcg gggcggttct acatcggcaa cggcgccgtc cggttcctgg
     3901 gcgcggaagg ctccgtgttc gacaagacct atctcgtccc cgacaaactc aatctggccg
     3961 tctccctcac gcgggaatat ctcaccaccg acgcccaggg acacctgatc gactccgtgg
     4021 accttcccga agccgcggcg gacgccccgc gcgtggaaaa accgaaaggc aaggaagccc
     4081 gcgcctatcc cgcgcaggac aacacccgtc tgccggggct gttcacctgg accaacctga
     4141 tctacgccac cgtgttcctg ggtctgctga tcctggcggc caccatcttc atctggaacc
     4201 cggcggtcca tctccagatc acggggctcc cctggctgag ccgcatcacg cgctacggca
     4261 ccttcttcat gatccgcggc atcaccgccg tgatcacctt catcgtgaac tatttcttca
     4321 tggcgttcgt cggcatactc cagtacgtgt tcttctacct cctcactcaa cggaaactga
     4381 tccgccagat ggacgaaaaa caggacatcg cgcccatcga cctgcctccc gagatcgccc
     4441 gcttccccga attcaacatc acggaggacc cctccacgct gaaggaaaaa ccgctcgtca
     4501 agctggggga aaacggccgg gtctacgtga accaccgggc gctcggcatc gcctacgcca
     4561 tgttccaaca gagccgctgg gtccacctca tccacaccgc caccttcggc ctggtgaaga
     4621 aggagaccct cggccgcatg cgtctgctcc tctttttcga atcggccctg gcccaggcca
     4681 agcaggacag tttcctggca ccgatccagc agatcccgct cctgggaatg ttcacccggc
     4741 gcggcgggcg atattcctgg ctgatccaag gcatcctgtg gcaaccgttc ctcatcgtgc
     4801 gcgggttctt ccgctccatc aagatgtggt tcgtccccga ggccgccgcg ctgggagaat
     4861 cgctggcgta cacgtccctg ccgacggacc tggcgcaaaa agggttcacc ctccattacg
     4921 ggtgcgtccg ca
//
LOCUS       DAOTFD010000197         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12541_length_4881_cov_3.805222, whole genome shotgun sequence.
ACCESSION   DAOTFD010000197 DAOTFD010000000
VERSION     DAOTFD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12541_length_4881_cov_3.805222"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="P5079_09550"
     CDS             complement(<1..1610)
                     /locus_tag="P5079_09550"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRY30264.1"
                     /translation="MFPFKDLPEFVRFLESKGELRRVRAPVDPELEITEIACRVVKEK
                     GPALLFENVAGSGFPLLINMFGTERRIEWTLGRPPSEIGVELKGFADDMMPPRLAAAW
                     KHRSLFRRALKMKPRVVARGPVGETDETPDLSRFPILKCWPKDGGRFLTYPLVFTQSP
                     VNGRRNIGIYRLHVFGPRRTGMHWQIERGGGFHYVEAESRKEPLPTLVVLGADPVLML
                     AGVLPLPEDMDELAFAGFLRGEPLRLTRLPGTDMDVPADAEIVLAGRIPPFQREMEGP
                     FGDHLGHYSHPAPFPVFEIERVYRRSRPIYPAAVVGLPPQEDKYMGNAAQEMLLPLIR
                     VLHPELTDFWAYYEAGFHNLAVAAVRQRYQREGLKTAFALLGEGQVSLTKCLVLVDPD
                     VDVRRWPDVLAALRRNFDPREDLLVLPGTSQDTLDFTGPKMNLGSKMILDATRDGDAA
                     RDGPRAPAFSGLPFPHRNWHDTFLVIQPGAPARLHLEKMVRDPAFRSFKFIAAVGGDV
                     PLEDDELLTWGIFSRFDCARDLVPAKAE"
     gene            complement(1734..2288)
                     /locus_tag="P5079_09555"
     CDS             complement(1734..2288)
                     /locus_tag="P5079_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013679561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRY30265.1"
                     /translation="MKIVLGVTGASGSIYAVEFLRRCANEELYLVVSRWGRSVLHQET
                     GLAVESLSTFAKKVFSNDDMNAPLASGSNAFDALLILPCSVTTMAKIAHGIGDSLLTR
                     CAEVALKERRRLILCVRETPWSAVDFENAHKLTLAGAVVMPVSPAFYQKPTSLPDLVG
                     SFVDKIRSVLGLPVESGWRSSELE"
     gene            complement(2290..3177)
                     /locus_tag="P5079_09560"
     CDS             complement(2290..3177)
                     /locus_tag="P5079_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRY30266.1"
                     /translation="MDTTASGAIMRVNGTERLQAYARFIKIEHSLFSLPVLFAGTVLA
                     AEAWPGWRVSGLVLLAGLGARTLALAVNRLLDRDLDRKNPRTAGRELATGVLSVGDAV
                     LVSTAGLAVYVWAAFRLSDFCLMWCWVPVLFFVIYPTLKRFTALSHFGLGLTWGLAAL
                     GGWFAVRPGFDGMWPAVILALFSFFWLSGFDIIYATLDEEFDRREGIYSLPAKLGRYR
                     ALQLSALLHWAAFFCLVALYASALRGVLAAFLMITAGLLLFLEHLLVDNVDLAFFKIN
                     VLAGFIVLAMVISGVRSEF"
     gene            3259..3912
                     /locus_tag="P5079_09565"
     CDS             3259..3912
                     /locus_tag="P5079_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRY30267.1"
                     /translation="MKKRRPTPKTGRSGLLVVFDIGNVLLRFDMERAVRNFEKLSPGL
                     GTRIAPFLRRPALSRRFERGKISGPALYRYLKKETGFRLSYPAFCLAFSDIFTPVREN
                     LRLMKKISAARPVAVLSNTNSIHWRHIHAHYPAFRHVRWPYSSHLLGHMKPDPRIFYA
                     LSRRTGFPLGRMILIDDRPENVRGARRIGMAAVHYRGQPLHRHAALKTFQLRKDARR"
     gene            3909..4577
                     /locus_tag="P5079_09570"
     CDS             3909..4577
                     /locus_tag="P5079_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY30268.1"
                     /translation="MSLYFRQLPVGPMQNFAYLVGDEKTRKCWVVDPAWEGKKIRKTA
                     EKDGYAVAGLLLTHAHYDHCNAVEELLSPKDLPLYVHKDEVAALQKYADTGIFGPLPV
                     PRLAPVSSGDKISLGDVALSFLHTPGHSPGSQCLLLDDRVLSGDTLFIGACGRSDLPG
                     GDPRRLYDSLYRTLAALPGHLMLFPGHDYSPSDTASTLEKERRTNRALQAKSTADFLA
                     WLGR"
     gene            4581..>4881
                     /locus_tag="P5079_09575"
     CDS             4581..>4881
                     /locus_tag="P5079_09575"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011935877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY30269.1"
                     /translation="MTGLVFRKIRRAPSYARFKDLLTRSRCTRCGLSRGRTNIVVDRG
                     NPRAKILVVGEAPGRHEDEQGKAFVGQAGKLFDKILGAAGLNSDRDLLITNIVKCR"
BASE COUNT          947 a         1687 c         1488 g          759 t
ORIGIN      
        1 aactcggcct tggccggcac caagtcgcgc gcgcagtcga accgcgagaa aatgccccac
       61 gtcagaagtt cgtcgtcctc caacggcacg tcgccgccga ccgccgcgat gaacttgaac
      121 gagcggaagg cgggatcgcg gaccattttt tccaagtgga gacgggccgg ggcgccgggc
      181 tgaatcacca ggaaagtgtc gtgccagttg cggtgcggaa acggaagtcc cgaaaacgcc
      241 ggcgcccgag ggccgtcgcg cgcggcgtcg ccgtcccgcg tcgcgtccag aatcatcttg
      301 ctccccaaat tcatcttcgg gccggtgaag tccagcgtgt cctgggaggt gccgggcagg
      361 acgagcaggt cttcccgcgg gtcgaagttc cgccgcaacg cggccaggac gtccggccaa
      421 cggcgcacgt ccacgtccgg atccaccaac accaggcatt tcgtcaacga cacctggccc
      481 tcccccaaca gggcgaaggc ggttttcagc ccttcccgct ggtaacgctg gcgcaccgcc
      541 gccaccgcca ggttgtggaa ccccgcctca tagtaggccc agaaatccgt cagttcggga
      601 tggagcacgc ggatgagcgg aaggagcatt tcctgggcgg cgttgcccat gtatttgtcc
      661 tcttgcggag gcaggccgac cacggcggcg ggatagatcg gccgggaccg gcgataaacg
      721 cgctcgatct cgaaaacggg gaacggggcc gggtgggaat agtggcccag atggtccccg
      781 aacggtccct ccatttccct ctggaacggc gggatgcggc ccgccaggac gatttcggcg
      841 tcggccggca catccatgtc ggtgccgggc agccgggtga gcctcaaagg ctcgccccgc
      901 aaaaaaccgg cgaacgccag ctcgtccatg tcttccggca aaggcaacac cccggccaac
      961 atcaacaccg gatcggcccc cagcaccacc agggtcggca aaggctcttt ccggctctcg
     1021 gcctcgacgt agtggaaacc gccgccgcgc tcgatctgcc aatgcatgcc ggtgcgccgc
     1081 gggccgaaga cgtgcagacg gtagatgccg atgttgcgcc ggccgttgac gggactctgc
     1141 gtgaacacca gggggtaggt gaggaaccgg ccgccgtcct tcggccaaca tttgaggatg
     1201 gggaaccgcg acaggtccgg ggtttcgtcc gtctcgccca cggggccgcg ggccacgacg
     1261 cgcggtttca tcttcagcgc ccgccggaaa agggaccgat gcttccaggc cgcggccagg
     1321 cgcggcggca tcatgtcgtc ggcaaaccct ttgagttcca cgccgatctc cgacggcggc
     1381 cggccgagcg tccattcgat ccttcgttcg gtgccgaaca tgttgatcag gagcgggaat
     1441 ccggaacccg ccacgttctc aaagaggagg gcggggcctt tttccttcac cacgcggcag
     1501 gcgatctcgg tgatctccag ttccgggtcc accggcgccc ggacgcgccg cagttcgccc
     1561 ttcgattcca ggaaccggac gaattccggc aggtccttaa aagggaacat ggcgcatgtc
     1621 gcgtgaggaa aacacaggtg ggtgggaaat gggaagcctc gcttttcgcc ggcgggaagg
     1681 gaaccccgga ttcaaccccg cgtcgttaag ccgggtctcc ggagaacggc cttttactcg
     1741 agctcgctgg aacgccagcc gctttcgacg ggcagtccca ggacgctgcg gattttatcg
     1801 acgaaggaac cgaccaggtc gggaagcgac gtgggcttct ggtaaaacgc gggggacacc
     1861 ggcatgacca cggcgccggc cagggtcagt ttatgggcgt tctcgaaatc caccgccgac
     1921 cacggggtct cccgcacgca caggatgagc cggcgcctct ccttgagggc cacctcggcg
     1981 cagcgcgtca acaggctgtc gccgatgccg tgcgcgatct tggccatggt cgtcacggaa
     2041 cagggcaaga tcaacagcgc gtcgaacgcg ttggagcccg acgccagcgg ggcgttcatg
     2101 tcgtcgttgg aaaaaacctt tttcgcgaag gtggacaggc tctccaccgc gagcccggtc
     2161 tcctgatgga gcaccgaacg gccccaacgc gacacgacga ggtaaagctc ctcgttggcg
     2221 cagcgccgca ggaattcgac ggcgtagatg gaaccggagg ccccggtgac gccgagaacg
     2281 attttcatat cagaactcgc tccgcacgcc ggagatgacc atggccagca cgatgaatcc
     2341 ggcgagcacg tttatcttga aaaaagccag atcgacgttg tccaccagca gatgttccag
     2401 gaagagcagg agccccgccg tgatcatgag gaacgccgcc aagacgccgc gcaacgcgct
     2461 ggcatagagc gccacgaggc agaagaacgc cgcccaatgg agcagcgccg agagttgcag
     2521 ggcccggtac cggccgagct tggccggcag agaatatatc ccttcccggc ggtcgaactc
     2581 ctcatccagc gtggcgtaga tgatgtcgaa cccggagagc cagaagaaac tgaagagcgc
     2641 caggatgacc gccggccaca tgccgtcgaa ccccggccga accgcaaacc aaccgcccag
     2701 ggccgccaac ccccaggtga gccccagccc gaaatgggac agcgcggtga accttttcaa
     2761 cgtcgggtag atgacgaaaa agagaaccgg gacccagcac cacatcaggc agaaatcgga
     2821 gagacggaaa gcggcccaga cgtagacggc cagtccggcc gtcgacacga ggaccgcgtc
     2881 cccgacgctg agcacaccgg tggccaattc ccggcccgcg gtgcgcggat ttttccggtc
     2941 gaggtcccgg tccaggaggc ggttcaccgc caaggccagc gtccgggcgc cgagtcccgc
     3001 cagaaggacg agcccgctca cccgccagcc cggccaggcc tcggccgcca acaccgttcc
     3061 cgcaaacaaa accgggaggg agaaaagcga atgctcgatc tttatgaatc gggcgtaggc
     3121 ctgcagccgt tccgttccgt tgaccctcat gatcgctccg gatgccgtgg tgtccattat
     3181 ttcgttacta tagcaaattg ccccgcccga cgacagttct ttggacaccc gtcccccaac
     3241 ttgctaaaat cttccctcat gaaaaaacgc cgccccaccc caaaaacggg ccgatccggc
     3301 ctgctcgtcg tgttcgacat cggcaacgtg ctccttcggt tcgacatgga acgggccgtc
     3361 cgcaatttcg aaaaactctc gccgggactg ggcacccgga tcgcgccgtt cctccggcgg
     3421 ccggcgctca gccgacggtt cgagcgggga aagatatccg gccccgccct gtatcgttac
     3481 ctgaaaaaag agaccgggtt ccggctctcc taccccgcct tttgcctggc cttcagcgac
     3541 atcttcaccc ccgtccggga gaacctccga ttgatgaaaa aaatttccgc cgcccggccg
     3601 gtcgccgtcc tctccaacac caacagcatc cattggcggc acatccacgc ccattacccc
     3661 gccttccgcc acgtccgctg gccgtacagt tcgcacctcc tcggtcacat gaaaccggac
     3721 ccgcgcatct tctacgccct ttcccggcgg accggttttc cgctgggccg gatgatcctc
     3781 atcgacgacc gccccgagaa cgtgcgcggc gcgaggagga tcggcatggc cgccgtccat
     3841 taccggggac aaccgctcca ccgccacgcc gccctgaaaa ccttccagct ccggaaggac
     3901 gcccgccgat gagtctctac ttccgccaac tcccggtcgg ccccatgcag aacttcgcct
     3961 atctcgtcgg cgacgaaaaa acccggaaat gttgggtggt ggaccccgcc tgggagggga
     4021 agaagatccg caagacggcg gaaaaggacg gctacgccgt cgccggtctc ctcctgaccc
     4081 acgctcacta cgaccattgc aacgcggtcg aggaactcct ctccccgaag gacctcccgc
     4141 tctacgtcca caaagacgag gtcgccgctc tccagaaata cgccgacacc ggcatcttcg
     4201 gccccctgcc cgtcccgcgg ctcgcgccgg tgagttccgg cgataaaatt tcgctgggcg
     4261 acgtcgccct ctcctttctg cacacgcccg gccactcgcc cggctcgcag tgcctcctcc
     4321 tcgacgaccg cgtcctgtcc ggcgatacgc tgttcatcgg cgcctgcggc cgctcggacc
     4381 tgccgggggg ggatccgcgc cgcctctacg acagcctcta ccgaaccctc gccgccctgc
     4441 ccggccactt gatgcttttc ccaggccacg actactcgcc ctcggacacg gcgtccaccc
     4501 tggagaaaga gaggcgcacc aaccgcgcgc ttcaggccaa atccacggcg gatttcctgg
     4561 cctggctggg gcgctagcgc atgaccggcc tcgtcttccg taaaatccgg cgggccccct
     4621 cctacgcccg tttcaaagat ctcctgaccc gttcccgctg cacgcggtgc ggtctgtcgc
     4681 gggggaggac gaacatcgtg gtggaccgcg ggaacccgcg ggcgaaaatc ctcgtcgtcg
     4741 gcgaagcgcc cggccgccac gaggacgagc agggaaaagc cttcgtgggc caggcgggga
     4801 aactcttcga caagatcctg ggggcggccg ggctcaactc cgaccgggac ctgttgatca
     4861 ccaacatcgt caaatgccgg c
//
LOCUS       DAOTFD010000198         4877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12563_length_4877_cov_5.110328, whole genome shotgun sequence.
ACCESSION   DAOTFD010000198 DAOTFD010000000
VERSION     DAOTFD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12563_length_4877_cov_5.110328"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="P5079_09580"
     CDS             <1..879
                     /locus_tag="P5079_09580"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRY30270.1"
                     /translation="DSDIVALNGVRGDLSRLGVPLAWIARMDADTSPDDRLWAIADAV
                     SCRVPPETDAAAFLELPSIKRTRAEEKALFLDAEDVGTLLAVGPELVRAMPGRLIFGL
                     RHDDTSRLLHQIRYLAAQAWLKDAGCPIHLYVPHTSDNDHGVIDSSILAGSLLCDGIG
                     DSVQFQSGDAGPDSLETAYAVLQCAGARRTRTEYISCPSCGRTLFDLRATAERIIQQT
                     RHLKNVKIAIMGCIVNGPGEMADADFGYVGAGPGKINLYIGKECVEKGIPTEDAVEHL
                     LSLIKKSGKWAEPTPS"
     gene            855..2000
                     /locus_tag="P5079_09585"
     CDS             855..2000
                     /locus_tag="P5079_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY30271.1"
                     /translation="MGRAHTLMNPSSVTDRSLLFRVFFFGVFLFLIYQLIRVLSPFFT
                     AIAGAGTLCLIFYPLHVRLTRKLQNRPSLAAGLSTALLLLIVVLPVVLFAWILIREAA
                     AVYPTARAWAQNLKNLSDPAWSAALPRPVHLLWLKANQVFSVYAIDVQDILLNNLDQL
                     GGTLSTFGTKILKNLAVFVFDMFVLVFSLFFFFRDGHAFVRWGIDHVPMETGHKDMIL
                     ERLDRTLSAVVRGVFVTAAVQGLLAGIGFLLAGVRFPVLLGFTTAFLAPIPFVGAAAV
                     WLPLGLYTLSMVSLGKGLFLLLWGFFVVSLVDNFLRPFLIGEKANLPVLLLFFGILGG
                     IQVYGFMGILIGPLMIAAVISFTKIYREQMQLSAAEKKVPPAPPNVP"
     gene            1990..2826
                     /locus_tag="P5079_09590"
     CDS             1990..2826
                     /locus_tag="P5079_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY30272.1"
                     /translation="MSPDIPPAGTITSRKHPLVQRLREIRDSKDPSRLFVEGVSLAKE
                     LLSSSLTTTHFFYTKDFADGPPPPVFQKMIAEAKTPGALLSDDVMSYVSDLKTPPGLI
                     LLAERPSFRLEDLAKKNSPLLVLLDALQLPQNVGAVVRAAEAAGADGLVQTGEGTDPY
                     GPKSLRGSAGSAFRLPIVHDIALAEATDALTRAGIRCVVAAAKGKTAYTDWDWKKPTA
                     VLFGTESAANPKKQISEISETLPSLHIPMHSGVESLNVSVAAGIILFEARRQRRDADS
                     HG"
     gene            2819..3985
                     /locus_tag="P5079_09595"
     CDS             2819..3985
                     /locus_tag="P5079_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRY30273.1"
                     /translation="MVETGLDVFLRHKAGPWLGKRAGLIAHQASVDQNLQPAAEALDK
                     LRGFRLATLFAPEHGLDGALQDQIPVERKTDPARRRPVYSLYGKNRLSPPRQALARLD
                     CLLFDLQDIGVRYYTFIWTMVLAMKAAAAAGIPFVVLDRPNPLGGEIVEGNIPDPSFA
                     SFVGLLPIPVRHGMTCGELARYFNDKYRLGCDLTVVAMRGWKRRDWFDETGLPWVMPS
                     PNMPTLDTATVYAGMCLLEATNLSEGRGTTRPFEVAGAPFIEAEKLRKALQKLALPGV
                     LFRPCAFSPTFNKWAGRTCRGVQLHVTDRKKFLPYRTGLCFLQTVRRLWPGRFRWKKP
                     PYEYETRKRPMDILCGTEGLRKAVDAGKDIRELSKAWEGPLRGFLSERKKALLY"
     gene            complement(3979..>4877)
                     /locus_tag="P5079_09600"
     CDS             complement(3979..>4877)
                     /locus_tag="P5079_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY30274.1"
                     /translation="DDTSWPVDEIRGAMVTEQALNPDRVFAVHPPALLDGYGPLAVWK
                     MNRPEDIAAAVARFTDYPFDAAALSLSTETAAYQVNGLVKYRLFDAYRRDESLARIAE
                     HLLREKDFDLFMITFRGVDFTSHGFWKFREPDKYPVPPEQVAYFRDVVDRYYRYADEL
                     LGRMLARTDERTAVLVVSDHGFQGRETDIINYRDVSSSGRHNLEGLFLLSGAPFRRTE
                     GFQEASLYDVAPTVLYLMGLPLTDEMIGKPLVQWMDEDFVKARRIKKIGSYGPYRAMR
                     RKAGSDMTREEVELMKQLGYLQ"
BASE COUNT          887 a         1691 c         1349 g          950 t
ORIGIN      
        1 gattcggaca tcgtcgcgct taacggggtc cgcggcgatc tttcgcgtct cggggtcccc
       61 ctggcctgga tcgcccgcat ggatgccgac acctccccgg acgaccgtct ctgggccatc
      121 gccgacgccg tttcctgccg cgtccctccc gaaacggacg ccgccgcttt tctggaactt
      181 ccgtcgatca aaagaacacg ggcggaggaa aaagctttgt tcttggacgc ggaggatgtc
      241 ggaaccctcc tcgccgtcgg tccggaattg gtccgggcca tgcccggtcg tctgattttc
      301 ggcctgagac acgacgacac ctcgcggctg ctccatcaga tccgctacct tgccgcccaa
      361 gcctggctga aggacgccgg ctgtcccatc catctctacg tgccgcacac gtccgacaac
      421 gaccacgggg tcattgattc ctccatcctc gccggttccc tcctctgcga cggcatcgga
      481 gacagcgtgc agttccaatc cggcgacgcc gggccggatt ctttggaaac ggcctacgcg
      541 gtgctgcaat gcgccggggc gcgacgcacg cgcacggaat acatctcctg cccctcctgc
      601 ggccgaaccc tgttcgacct gcgcgccacg gcggaaagga tcattcaaca gacccggcac
      661 cttaaaaatg tcaaaatagc gatcatggga tgcatcgtca acggacccgg ggagatggcc
      721 gacgccgatt tcggctacgt cggcgccggc cccgggaaaa tcaatctcta catcggcaaa
      781 gaatgcgtcg aaaaaggcat ccccacggag gacgcggtgg aacaccttct ctcgctcatc
      841 aaaaaatccg gcaaatgggc cgagcccaca ccctcatgaa tccttccagc gtaacggacc
      901 ggtctctcct cttccgcgtt ttcttcttcg gcgtcttcct ttttttgatc taccagctta
      961 tccgcgtcct atccccgttc ttcacggcca tcgccggagc aggaacgctg tgcctcattt
     1021 tttatccgct tcacgtccgg ctgacacgga aactccagaa tcgccccagc ctcgccgccg
     1081 gcctcagcac cgcgctgctg ctcctcatcg tggtcctccc cgtcgtcctt ttcgcctgga
     1141 tactgatccg ggaagccgcc gcggtctatc ccaccgcccg ggcctgggcc caaaacctca
     1201 agaacctctc cgatcccgcc tggtccgccg ccctgccgcg gccggtccat ctgttgtggt
     1261 tgaaagcgaa ccaggttttt tccgtctatg ccatcgacgt acaggacatc ctcctgaaca
     1321 acctcgacca gctgggcgga accctctcca ccttcggcac aaagatcctc aagaacctgg
     1381 ccgtcttcgt gttcgacatg ttcgttctcg tcttctccct cttcttcttt ttccgggacg
     1441 gccatgcctt cgtgcgctgg gggatcgatc acgtccccat ggagaccggc cacaaggaca
     1501 tgatcctcga acggctcgac cgcacgctct cggcggtggt gcgcggcgtc ttcgtaacgg
     1561 cggcggttca ggggctgctg gcggggatcg gcttcctctt ggcgggcgtc cgattcccgg
     1621 tgctcctggg cttcacaacc gctttcctcg cccccatccc gttcgtcggc gcggcggccg
     1681 tctggctgcc cctggggctg tacacgcttt cgatggtctc cctcgggaaa ggactgttcc
     1741 tcctcctctg ggggttcttc gtcgtcagcc tcgtggacaa ttttctgcgt cctttcttga
     1801 tcggcgaaaa agcgaacctg ccggtgctcc tgctcttctt cggcatcctg ggcggaatcc
     1861 aggtctacgg tttcatgggc atcctgatcg gccctctgat gatcgccgcg gtgatctctt
     1921 tcaccaaaat ataccgggaa cagatgcagc tctccgccgc cgaaaaaaag gttccccccg
     1981 ccccaccgaa tgtcccctga catccctccc gcgggaacca tcacctcgcg caaacatccc
     2041 ctggttcaac gcctgcgcga gatccgcgac agcaaggatc cctcccggct cttcgtcgaa
     2101 ggcgtctccc tcgcgaaaga gctcctctcc tcctctctga cgacgaccca tttcttctat
     2161 acgaaggatt tcgccgacgg gcccccgccc ccggtttttc aaaagatgat cgccgaggcg
     2221 aaaacgccgg gcgcgcttct gtcggacgac gtgatgtcct acgtttccga cctcaaaacg
     2281 ccgcccggcc tgatccttct ggccgaacgc ccctcgttcc gcctcgagga tcttgccaag
     2341 aaaaattccc ccctgcttgt cctcctggac gccctgcaac tgccccaaaa cgtgggggcg
     2401 gtcgtccgcg ccgccgaggc ggccggggcc gacggactcg tccagaccgg ggaagggacc
     2461 gacccctacg gccccaaatc cctgcgcggc tccgccggga gcgccttccg attgcccatc
     2521 gtccacgaca tcgccctggc ggaggcgacc gacgccctga cgcgggccgg catccgctgt
     2581 gtggtcgccg cggcgaaagg gaaaaccgcc tacaccgatt gggactggaa aaaaccgacc
     2641 gccgtccttt tcggcacgga gtcggcggca aaccccaaaa aacaaatatc ggaaatatcg
     2701 gaaaccctcc ccagcctcca catccccatg cattccggcg tggagtccct caacgtgtcg
     2761 gtcgcggccg ggatcatcct ctttgaggca cgccgccaac gccgggacgc cgattcccat
     2821 ggttgaaacg ggactcgacg tctttctccg gcacaaagcc ggaccgtggc tgggaaaaag
     2881 ggccgggctc atcgcccatc aggcctccgt cgatcagaac ctccaacccg ccgcggaggc
     2941 gctggacaaa ttgcgcgggt ttcgtctggc cacgctgttc gcccccgaac acgggctcga
     3001 cggcgctctt caggaccaga tcccggtgga acgaaaaacc gaccccgctc gtcgacggcc
     3061 ggtctacagt ctttacggaa agaaccgtct ctcccctccc cgccaggcgc tcgcccgcct
     3121 cgactgcctc cttttcgatc tccaggacat cggcgtgcgt tactacacgt tcatctggac
     3181 catggtgttg gcgatgaaag ccgccgcggc cgcggggatc cccttcgtcg tcttggaccg
     3241 accgaacccg ctcggcggcg aaatcgtgga aggaaacatc cccgacccct ctttcgcttc
     3301 tttcgtcggc ctcctgccga tccccgtgcg gcacgggatg acctgcgggg agttggcccg
     3361 ctacttcaac gacaaatatc gtctggggtg cgatctgacg gtggtggcga tgcgtggatg
     3421 gaaacgacgg gactggttcg atgagacggg cctgccgtgg gtcatgccgt ctcccaacat
     3481 gcccacgctg gacaccgcca cggtctacgc ggggatgtgc ctcctggaag cgacgaacct
     3541 ttcggaagga cgcggcacga cgcggccgtt cgaggtcgcg ggggccccgt tcatcgaggc
     3601 ggaaaaactg cggaaggcgc ttcagaaact ggcgctgccg ggcgttcttt tccggccctg
     3661 cgctttcagc cccactttca acaaatgggc cgggcggacg tgccgggggg tgcagctgca
     3721 cgtcacggac cgaaaaaaat ttcttcccta ccggaccggc ctctgttttc tgcagacggt
     3781 gcggcgcctc tggccgggac gtttccgctg gaagaaacca ccctacgagt atgagacccg
     3841 caaaagaccg atggacatcc tctgcggcac ggagggcctc cgaaaagcgg tggacgccgg
     3901 gaaggatatc cgtgaattga gcaaggcgtg ggaggggccg ctccgcggct tcctctcgga
     3961 acggaaaaaa gcgctccttt actgaaggta tccgagttgt ttcatcaact cgacttcctc
     4021 cctcgtcata tccgaccccg ccttccttct catcgcgcga tacggcccat aggacccgat
     4081 cttcttgatt ctccgcgcct tcacaaaatc ctcgtccatc cattgaacca gcggctttcc
     4141 gatcatttcg tccgtcaacg gaagccccat cagataaagc acggtcgggg cgacatcgta
     4201 aagggaggct tcctgaaaac cctccgtcct ccggaaaggg gcgcccgaca gcaagaaaag
     4261 accttccaaa ttgtgccgac cggacgatga gacatccctg tagttgatga tgtccgtctc
     4321 cctcccttga aaaccgtggt cggaaaccac aaggaccgcg gtgcgctcgt cggtccgggc
     4381 caacatcctc cccaacagtt catccgcata gcggtaatac cgatcgacga cgtctcggaa
     4441 gtacgccacc tgttccggag ggacgggata tttgtccggt tcccggaact tccaaaaacc
     4501 atggctcgta aaatccaccc cgcggaacgt gatcatgaaa agatcgaaat ccttttcccg
     4561 gagaaggtgt tcggcgatgc gcgccaagct ctcgtccctc cgataggcgt cgaagagccg
     4621 gtatttcacg agaccgttca cctgatacgc cgccgtctcc gtgctcaggc tcagggccgc
     4681 ggcgtcgaac ggatagtccg tgaaccgggc gacggccgcg gcgatgtctt ccggccggtt
     4741 cattttccag accgccagcg gcccgtaccc gtccagaagg gccggcggat gaacggcgaa
     4801 aacacggtcc gggttgagcg cctgctccgt caccatggcc ccccggattt cgtccaccgg
     4861 ccaggaagtg tcgtccc
//
LOCUS       DAOTFD010000199         4846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12690_length_4846_cov_4.132123, whole genome shotgun sequence.
ACCESSION   DAOTFD010000199 DAOTFD010000000
VERSION     DAOTFD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12690_length_4846_cov_4.132123"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="P5079_09605"
     CDS             <1..181
                     /locus_tag="P5079_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRY30275.1"
                     /translation="AGRLRTAVEKNSYVLVEAVAEELARAALAAAGARTVSVRVRKKV
                     LAGIDDVEVEINRSA"
     gene            complement(173..574)
                     /locus_tag="P5079_09610"
     CDS             complement(173..574)
                     /locus_tag="P5079_09610"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRY30276.1"
                     /translation="MFRVTREIHFCYGHRLMRYAGKCRHPHGHNGRAQVELSSPRLDR
                     RGMVMDFDEIKKKIQTWIDDHLDHRMILCQADPLAKLLQKAGEPVYLLKENPTAENIA
                     REIFRVARGQGLPVRRVTLWETEKSSASYSR"
     gene            714..935
                     /locus_tag="P5079_09615"
     CDS             714..935
                     /locus_tag="P5079_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30277.1"
                     /translation="MNRGEKMAGLVLGIALFAGLFYLIYDSFITRPVEYEEPVEGLEQ
                     TMEAGEEEAPPEEELTDEEEAAAPAASPQ"
     gene            969..1655
                     /locus_tag="P5079_09620"
     CDS             969..1655
                     /locus_tag="P5079_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30278.1"
                     /translation="MAGRDLFKEIIEQTQVVKFPRHRLSTFGLSEIHYNLVSAVSQVP
                     PRSHLRMGLVVAERPRILTPDALANQFEGFGEHSESFERWLKETFHEEFRGLEYRFRN
                     QLSETAPHSMDARELVEKIQKDIEGRDTPQAALIFGPEKGWQFALMKFLLEETSQSFP
                     MNARELEERGLFNPLEAAMNRRRYEIENLFLQARNNPARVGLLGQKLREYKLFSEYQD
                     RFFSLVKGTH"
     gene            1727..2386
                     /locus_tag="P5079_09625"
     CDS             1727..2386
                     /locus_tag="P5079_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRY30279.1"
                     /translation="MASSVKPLWSKDKAFEWIKLHKMVAIVRTRHAMQARGTVEAILK
                     GGFKLVEVAMTVLGATDVLREFSSRPGVLVGAGSVLSEKMAKTALASGAQFLMTPHTD
                     PAILKLAQRAKVLCIVGALTPTEVVRAWSLGADVVRVFPVRAVGGAEYVRALKELFTD
                     VPLMPSGGVTLENVRDYFMAGSLAVGAAGALMNDDAVEASRWADITTQARKFQEMLQR
                     I"
     gene            2522..3445
                     /locus_tag="P5079_09630"
     CDS             2522..3445
                     /locus_tag="P5079_09630"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRY30280.1"
                     /translation="MKVLVADPRGFCAGVVRAVDVVEIALKRFSAPVYVRKEIVHNRS
                     VVEAFRARGVVFIDNLDEVPSGSLVVFSAHGVAPEVREQARRRGLRVIDATCPLVTKV
                     HLEAHRFLRDGYSLVLIGHKEHDEVVGTMGEAPGRIQLVESVDDVATLSVPDPARVVI
                     LTQTTLSLDESREIVAAIRRRFPQARTPPKDDICYATQNRQDAVKELLRRGVELLLVV
                     GSKNSSNSQRLVEVARQAGCRAHLLDNAAEIDPSWFSETSVVGVTAGASAPEYLVRDV
                     LDLLREKFGAVVEEVRVREENVHFSLPKELM"
     gene            3462..4184
                     /locus_tag="P5079_09635"
     CDS             3462..4184
                     /locus_tag="P5079_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY30281.1"
                     /translation="MIDRRLNYGRHQIRKFLAAAPFHTVLDLGAGAGEDLLMAREVDP
                     SAELHAVEIEPSFVRQLRGCGIRTHEVDIETGRCPFPDGSLDVVIANQVLEHAKEIFW
                     IAHEVSRVLAVGGRFIVGVPNLASLHNRFLLALGRQPTPIQNNSAHIRGYTRNDLCRF
                     LESAFPGGYALRAWGGSNFYPFPPFLAKPLAALFPNMAWGVFLHFEKKKPYRRQFLEF
                     PITQNLQTKFFLGDGPAEGGKG"
     gene            4181..4738
                     /locus_tag="P5079_09640"
     CDS             4181..4738
                     /locus_tag="P5079_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30282.1"
                     /translation="MNERFLELARRNEIFPQSADLDLADRLIRRSMKFFAAASRPRSA
                     SSEETTLFCYLALTLLLRSWLAAKGYRPRGGDSGGVVGEAVGAMPGSPAEEIAKVYGD
                     LRSYWVAGFLEPDMPLPVLSKEASLRGSRRLAAALRRRMVKEFPGLKKSFGAGKKEAP
                     FASPERTFRGGGAPARSAGSAKKRG"
BASE COUNT          848 a         1426 c         1633 g          939 t
ORIGIN      
        1 ggccggccgg ctccggacgg ccgtcgaaaa aaacagctat gtgttggtgg aggcggtggc
       61 ggaggagctg gcgcgcgccg cgttggcggc cgcgggggcg cggacggtgt cggtgcgggt
      121 gcgcaaaaaa gttttggcgg ggatcgacga cgtcgaggtg gagatcaacc gttcagcgtg
      181 agtagctggc ggaggatttt tccgtctccc agagggtgac ccggcggacg gggagcccct
      241 ggccgcgggc cacgcggaat atttcgcggg cgatgttctc ggcggtgggg ttttccttta
      301 aaaggtagac cggctccccg gctttttgaa gaagtttggc gaggggatcg gcctggcaga
      361 ggatcatccg gtggtctagg tggtcgtcga tccaggtttg gatcttcttt tttatttcgt
      421 cgaagtccat caccatgccg cgccggtcca ggcgggggga ggaaagttcc acctgcgccc
      481 ggccgttgtg cccgtgcgga tgccggcact tgccggcgta gcgcatcagc cggtgcccgt
      541 aacagaaatg gatttcccgc gtcactcgaa acaaggcccc tccgccgttc ccgatttgcc
      601 ccatgaatta tgctaatata agaattgaga gagggtcaag aagaatacgt tcctccgtcg
      661 ggaaaaagcg gggcagggcg aagggcgcgt aaatatcggg tggaggtgtt cgcatgaacc
      721 gcggtgaaaa aatggccggg ctggtcctgg ggatcgcctt gttcgccggt cttttctatc
      781 tcatttacga ttctttcatc acccggccgg tggagtatga ggagccggtg gaggggctgg
      841 aacagacgat ggaggcggga gaagaagagg ccccgcccga agaggaattg acggacgagg
      901 aggaggcggc ggcgccggcc gcttcgcccc aataaccgcc cgaccggggc tttttccgga
      961 ggcttttcat ggccggccgt gatttgttca aggagatcat cgaacagacc caggtggtga
     1021 agtttccccg ccaccgcctc tctacgttcg gtctcagtga aatccattac aacctggtga
     1081 gcgccgtttc ccaagtcccg ccgaggtccc atctgcgcat ggggctggtg gtggcggaac
     1141 ggccgcgcat cctcaccccc gacgccctgg ccaaccagtt cgaggggttc ggcgagcact
     1201 cggaatcctt tgagcgctgg ctgaaggaaa ccttccatga agagttccgc ggcttggaat
     1261 accggttccg caaccaattg tcggagaccg ccccccattc gatggacgcc cgcgaactcg
     1321 tcgaaaagat ccagaaggac atcgaggggc gcgacacgcc ccaggccgcc ctgatcttcg
     1381 gccccgaaaa gggctggcag ttcgccctga tgaaattcct tctggaggaa acctcccagt
     1441 ccttccccat gaacgcccgc gaactggagg agcggggtct tttcaacccg ctggaggccg
     1501 ccatgaaccg tcggcgatat gaaatcgaga atctcttcct ccaggcgcgc aacaaccccg
     1561 cgcgggtggg ccttttggga cagaaactgc gggaatacaa acttttttcc gaataccagg
     1621 accgcttttt ttcgttggtg aaagggaccc actaaaagcc gtttcgcccg tatccccgct
     1681 ttcggaattt tttgcggtct caagacaacc gaggagggaa ttgctcatgg cttcttccgt
     1741 caaaccgctc tggtcgaaag acaaggcttt cgaatggatc aagcttcata agatggtggc
     1801 catcgtgcgc acccggcacg cgatgcaggc ccggggcacc gtggaggcca tcctgaaggg
     1861 cggcttcaag ttggtggagg tggccatgac ggtcctcggc gcgacggatg tcctccggga
     1921 attttcttct cggccgggcg tgctggtggg cgcggggtcg gtgctctcgg aaaagatggc
     1981 gaagacggcc ctggcttccg gcgcgcagtt cctgatgacc ccccacaccg atccggccat
     2041 cctgaagttg gcccagcgcg ccaaagttct gtgcatcgtc ggcgccctca ccccgacgga
     2101 agtcgtccgg gcctggtccc tgggggccga cgtggtgcgg gttttccccg tccgcgccgt
     2161 cggcggggcg gagtatgtcc gggccttgaa ggaactcttc accgatgtgc ctctcatgcc
     2221 cagcggcggg gtcactttgg aaaacgtgcg ggactatttc atggccgggt ccttggcggt
     2281 gggcgccgcg ggggccttga tgaacgacga cgccgtggag gcttcccgct gggcggacat
     2341 caccacccag gcccggaaat ttcaggagat gctccagcga atttaaacac cccgggttct
     2401 tcccttgggc cgcttccgcg ttttttcgaa cggctcccgt ccgcccgtcc gatttttgaa
     2461 cctgaccccg tcctgccggt cgaagacaaa atgctaaaat gccgatgaga gagacatgtc
     2521 gatgaaagtc ttggtcgccg atcccagggg gttctgcgcc ggcgtggtcc gggccgtgga
     2581 tgtggtggag atcgccctga aacggttttc cgctcccgtc tacgtgcgca aggagatcgt
     2641 ccacaaccgg tcggtggtgg aggctttccg cgcccgcggc gtggtcttca tcgacaacct
     2701 cgacgaggtc ccgtcgggga gcctggtggt tttcagcgcc cacggggtcg ctccggaagt
     2761 gcgggaacag gcgcgccggc ggggattgcg cgtcatcgac gccacctgcc cgctggtgac
     2821 gaaagtccat ctggaggccc atcgcttcct gcgggacggg tattcgctgg tcttgatcgg
     2881 ccataaggaa cacgacgagg tcgtcggcac catgggcgag gcccccggcc ggatacagct
     2941 cgtggagtcg gtggacgacg tggcgaccct ctccgtgccg gaccccgcgc gcgtggtgat
     3001 cctcacgcag acgacgttga gtctggatga atcgcgggag attgtcgcgg cgatccgccg
     3061 gcggtttccc caggcgcgca ccccgcccaa agacgacatt tgttacgcca cgcagaaccg
     3121 tcaggacgcg gtgaaggaac tgcttcgccg gggggtggaa ctccttttgg tggtgggctc
     3181 caagaacagt tccaattcgc agagattggt ggaggtggcg cgtcaggcgg ggtgccgggc
     3241 ccacctgctc gacaacgccg cggaaatcga cccgtcctgg ttttccgaaa cgagcgtggt
     3301 gggggtcacc gcgggcgcgt cggcgccgga atatctggtg agggacgttc tggatttgct
     3361 tcgtgagaag ttcggagccg tcgtcgaaga ggtccgggtg cgggaggaga acgtccattt
     3421 ctccctgccg aaggaattga tgtgatcgcc gggggtgcgg catgatcgat cgtcgtctga
     3481 actacggccg tcatcagata cggaaattcc tggcggcggc cccgttccat accgtgctcg
     3541 acctcggcgc cggcgccggg gaggaccttc tcatggcccg ggaagtcgat ccgtccgcgg
     3601 aactccacgc cgtcgagatc gagccgtcct tcgtccggca attgagggga tgcggcatcc
     3661 ggacgcacga ggtcgacatc gaaaccgggc gctgtccgtt tcccgacggg tcgctggacg
     3721 tggtgatcgc caaccaggtg ctggaacacg ccaaggagat cttctggatc gcccacgaag
     3781 tgagccgcgt cctggcggtg gggggccgct tcatcgtcgg cgtgccgaac ctggcctcgc
     3841 tgcataaccg ctttctgctg gcgttgggcc gccagccgac acccatccag aacaattcgg
     3901 cgcacatccg cggctacaca cgaaacgatc tttgccggtt cctcgagagc gcgtttccgg
     3961 gagggtacgc gctccgggct tggggaggca gcaattttta tccgttcccg ccgttcctgg
     4021 ccaaacccct ggccgcgctt tttccaaaca tggcgtgggg ggtgttcctg cattttgaaa
     4081 aaaagaaacc gtatcggcgc cagttcctgg aatttcccat aacccagaac ctgcagacga
     4141 agtttttcct gggagacggg ccggcggagg gagggaaggg atgaacgaac gttttttgga
     4201 actggcccgc cggaacgaga tcttccctca gtccgccgat ctggacctgg ccgaccggct
     4261 catccgccgg agcatgaaat ttttcgccgc ggcgtcgcgg ccgcggtccg cgtcgtcgga
     4321 ggagacgacg ctgttctgtt atctggccct gaccctgctc ctgcgttctt ggctggccgc
     4381 caaagggtat cgtccgcgcg gcggggattc cggcggcgtg gtgggggagg ccgtcggggc
     4441 gatgccgggt tcgccggcgg aagagatcgc caaggtctac ggggatttgc ggagctattg
     4501 ggtcgccggc tttttggaac cggacatgcc cctgcccgtt ttgtccaagg aggcgtctct
     4561 tcgggggagc cgccgtttgg ccgccgcctt gcggcgacgg atggtgaaag aattccctgg
     4621 cctgaaaaaa tccttcgggg cggggaaaaa ggaagccccc ttcgccagtc cggaaaggac
     4681 tttccggggc ggcggcgcgc cggcccgttc ggccgggtcg gcgaagaaga ggggatgaaa
     4741 aaaaacgcga tggcccggcg gtccccgcgg cggcccgtgt tggtgttccg gcatgtgccg
     4801 cacgaacatc tgggggagat cgccgtcgct ttccgtcggg cgaaac
//
LOCUS       DAOTFD010000200         4845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12692_length_4845_cov_5.153862, whole genome shotgun sequence.
ACCESSION   DAOTFD010000200 DAOTFD010000000
VERSION     DAOTFD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12692_length_4845_cov_5.153862"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5079_09645"
     CDS             complement(<1..319)
                     /locus_tag="P5079_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRY30283.1"
                     /translation="MDDAELFKSLHEAIRAGQDVALATVISADGSTPRGSGSKMLVYP
                     DGRIEGTVGGGKLEAACLQEARAALAAGRNKKVAYSLTPDGLGMECSGKNEIFIEVFV
                     GRIR"
     gene            complement(312..1370)
                     /locus_tag="P5079_09650"
     CDS             complement(312..1370)
                     /locus_tag="P5079_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HRY30284.1"
                     /translation="MPNLKIFFALALLGTAFSKSWAWLIPAALLATAGNWGRAKELRR
                     LKFWIFFFLASFLPFFLIRRQEMLLVSGRMFLRAVLLYNAVLLYVENVDAERMKPVLR
                     RAFGERLSLTLSVAVNILPVMRRIFLQTHGVFLLRAPSSRARLFGYVRFLRAVLSQSL
                     LAAERLAENMIVAEGARQNEVHIISGPHGSGKTTLAAALAEKFQERGWPVAGVLSPGA
                     FEAGRRVRIEARDAASGETRLLASRNGEVSDPIHEHGGFRFSKSGFEFAKESLLRRAA
                     GSIVFVDEIGPLELAGEGYAETLENLLHADAAALYLVVREGLVQDVTKKFKISQYTIL
                     RPPAWEDRAAKGTDHANG"
     gene            complement(1357..1953)
                     /locus_tag="P5079_09655"
     CDS             complement(1357..1953)
                     /locus_tag="P5079_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30285.1"
                     /translation="MNRDDYYTVAYVAVFGVLWGAVEATLGTILHAVGLPLKGTLLTA
                     VGTVIVLAGALRMPARRGLPLLGMGVVAVAVKTAAMGVLKINILLSILAEAALLQAGV
                     LILGYTLPGCLLGGIWACLWPMGQRIFIYGALFGEGLAVIFDEMTRTGSAWLGVPVKS
                     VTVLLLGLAAAHAFLGAVAGTTGWLLGRSLRKNRHAEP"
     gene            complement(1950..3290)
                     /locus_tag="P5079_09660"
     CDS             complement(1950..3290)
                     /locus_tag="P5079_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY30286.1"
                     /translation="MTAPFQEKTAGAAAVSEKFRWIVSLDSFSVQAPDGHHILNSVSL
                     QIVPGRFTALLGSSGAGKTTLLRAILHLLPTGAGWKTDGFLDRFPGLRVGWVAQNPGL
                     QLFRNFVHEELGFSRVENAAAFLEPLELASLAGRRCAELSQGEKTMVVLARSLHQNPH
                     LLVLDEVMVNLSAARRDWFRRLLADFVGCGGAVLAVEHSPELLDSADAVVRLADGKLS
                     AVSKSAAEQFLRPDMAAGTDRAPRVPADPALELRGLADPGIASTKDRPLNLQIHAGEI
                     LGISGPNGCGKTTLLNFLAGFQPPRAGNILWRGRIWKTFDARRGIVSFVTQEPLNQFF
                     TDTPEKELGFSIHPENADAAAELLDEFHLTARRNRPIHSLSYGEQQRLALAAALTGRP
                     QLLLLDEPTYGMDRNNLRLFARVLRKERERGCAVVLASHEQDLLKHWADGVVSL"
     gene            complement(3287..3715)
                     /gene="tsaA"
                     /locus_tag="P5079_09665"
     CDS             complement(3287..3715)
                     /gene="tsaA"
                     /locus_tag="P5079_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRY30287.1"
                     /translation="MRVILEPIGSVRSAVKTRRHMPNQGTTAEIVLRRKFRKALYRIT
                     ASPSLWVLCYFHKADRDVLRARRRRFPKDKKLRGVFSTHSPDRPNPLAMTRVKLLSCR
                     GGVLRVQGLDAIDGTPVLDIKSAKDDILVFGEKRPFSRNP"
     gene            complement(3716..>4845)
                     /locus_tag="P5079_09670"
     CDS             complement(3716..>4845)
                     /locus_tag="P5079_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY30288.1"
                     /translation="NFNTKRNEAVFSTNAGAFTGIDKDIEEEITNSYVTWQRTFGRSR
                     FYVRTFLTTDRDRSASSPLSRASTQGGSLEWQGELPLRAELFFGTDYEDSHIRSGSPS
                     SRATQQYVAPFIQLSLPVWHERFIFLAGVRYDNNSKFTDQWTPKYGVVWKILPDLRAR
                     ANLNYGFRAPTVTELYSTNTRAFGNAHLRPDKNRQHEIGLSYSKKAGGIDLSAYKNVE
                     TDMIVSQVPVAPLTYQGAAVQKQYRNVDGKSAYRGVEVRARYAFLSHFEAGAGYSYLD
                     PGTLTFHTDKNTAQSSLTYRDGRFSTTVDMTHAGGRYTLDNFTGKRAAYTVYHWSGGW
                     TFARHFTYYLRFNNITDQEYALNFYDPMPGRTYLTGLEMTF"
BASE COUNT          902 a         1494 c         1564 g          885 t
ORIGIN      
        1 gtcggatccg cccgacgaag acctcgataa atatctcgtt ttttccggaa cattccatgc
       61 cgaggccgtc gggggtcagc gaatacgcca cttttttgtt tcggccggcg gccagggccg
      121 cgcgggcctc ttggaggcac gccgcctcga gtttgccgcc gcctaccgtg ccttcgatcc
      181 ggccgtcggg gtagaccagc atcttcgagc ccgatccccg cggcgtggat ccgtcggccg
      241 aaatgacggt ggcgagggcc acgtcctgcc cggcccggat ggcctcgtga agacttttga
      301 acagctcggc gtcatccatt tgcatggtcg gttcctttgg cggcgcggtc ttcccacgcg
      361 ggaggccgca gaatggtgta ttgggagatt ttaaattttt tcgtcacgtc ttgcaccagc
      421 ccttcccgaa cgaccagata cagggccgcg gcgtcggcgt ggaggaggtt ttccagggtc
      481 tccgcgtaac cctccccggc gagttccagc gggccgattt cgtcgacgaa gacgatggat
      541 ccggccgccc ggcggaggag cgactctttg gcgaattcga atccggattt ggaaaaacgg
      601 aacccgccgt gttcgtggat cgggtccgag acctcgccgt tgcgggacgc caacaggcgt
      661 gtttcgcccg aagccgcgtc gcgcgcctca atgcgaaccc ggcggccggc ttcgaaggcg
      721 ccgggagaca ggacgcccgc caccggccag ccccgttcct ggaatttctc cgccaaagcg
      781 gcggccaacg tggtttttcc gctgccgtgc ggcccggaaa tgatatggac ttcgttttgc
      841 cgcgcgcctt cggccacgat catgttttcc gccaggcgtt cggcggccag cagggactgc
      901 gacaacaccg cccgcagaaa tcggacatag ccgaacagcc gtgcccggga cgacggggcc
      961 cgcaacagaa acacgccgtg ggtctgcaga aagatgcgcc gcatcaccgg caggatgttc
     1021 accgccacgc tcagcgtgag ggacaaccgt tcgccgaacg cccgccgcag cacgggtttc
     1081 atccgttcgg cgtcgacatt ttcaacgtac agcagcacgg cgttgtagag gaggacggcg
     1141 cgcaggaaca tccgtccgga gacgaggagc atctcttgtc gccggatgag gaaaaacggg
     1201 aggaaggagg cgaggaagaa gaaaatccag aacttgagcc gtctcagttc cttcgcccgg
     1261 ccccagttgc cggccgtggc gagcagcgcc gccgggataa gccaggccca gcttttggag
     1321 aaagccgttc cgagcagggc caatgcgaaa aatattttaa ggttcggcat gtcggttttt
     1381 tcgcagggat cggccgagga gccaaccggt cgtccccgcc accgctccca aaaaggcgtg
     1441 ggcggccgcc aatccgagga gcaggacggt gacacttttc accggcacgc ccagccaggc
     1501 gctcccggtg cgcgtcattt catcaaagat taccgccagg ccctccccga acaaggcccc
     1561 gtagatgaaa atcctctgcc ccatcggcca aagacacgcc cagatgccgc cgagcaggca
     1621 gcccgggagc gtgtacccca aaatcagcac gcccgcctgc aggagggccg cctccgcgag
     1681 gatggagagc agaatgttta tctttagaac ccccatggcc gcggttttaa ccgccaccgc
     1741 cacgaccccc atccccagga gcggcaatcc ccggcgggcc ggcattcgga gcgcgccggc
     1801 aagaacgatc accgttccga cggccgtgag cagggtgcct ttcaaaggca ggccgacggc
     1861 gtgaagtatc gtgcccagtg tggcttccac ggcgccccaa aggacgccga agaccgccac
     1921 ataggcgacc gtgtagtaat cgtcgcggtt catagggaaa caaccccgtc cgcccaatgt
     1981 ttcaaaaggt cctgttcgtg cgacgccagg acgaccgcgc atccgcgttc tctttctttc
     2041 cgcaacaccc gcgcaaagag ccgaagattg ttgcggtcca tcccgtaggt gggctcgtcc
     2101 aacaaaagaa gttgtggtcg gcccgtcagg gccgcggcca gggccaggcg ttgctgttcc
     2161 ccataactga gggaatggat cggccggttt cgccgggcgg tcagatgaaa ttcgtccagg
     2221 agttccgccg ccgcgtccgc gttttcgggg tgaattgaaa atcccagctc cttctccggc
     2281 gtgtccgtga agaactggtt cagcggttcc tgggtcacga aggagacaat tcctcgccgg
     2341 gcgtcgaagg ttttccagat tcgtccccgc caaaggatat ttccggcgcg cgggggctga
     2401 aacccggcca ggaagttgag gagggttgtt ttgccgcacc cgttcggccc cgagatcccc
     2461 agaatctccc cggcatggat ctggaggttc agcggccggt ctttagtcga ggcgatgccg
     2521 ggatccgcga ggccgcgcag ttccagggcg gggtctgccg ggacgcgcgg ggcacggtcg
     2581 gtcccggcgg ccatgtccgg ccgcaggaac tgttcggcgg cgctcttgga caccgcggag
     2641 agttttccgt ccgccaaacg gacgacggcg tcggccgagt ccaggagttc cggcgagtgt
     2701 tccaccgcga gcaccgcgcc gccgcatccc acaaagtcgg ccaaaaggcg cctgaaccag
     2761 tcccgccgcg ccgccgacaa attcaccatc acctcgtcca ggacaagaag gtgtgggttc
     2821 tgatgcagcg accgcgccag gacgaccatg gttttttcgc cctgcgacag ttccgcgcac
     2881 cgccggccgg ccagggaggc cagttccagc ggttccagaa aagccgccgc gttttccacg
     2941 cgggagaacc ccagttcttc gtggacgaaa ttgcggaaaa gttggaggcc gggattctgc
     3001 gccacccagc cgacgcgcaa gccgggaaaa cggtccagaa atccgtccgt tttccagccg
     3061 gcgcccgtcg gcaggaggtg gaggatggcc cgcaggagcg tggtttttcc ggccccggac
     3121 gatcccagga gcgccgtgaa gcggccgggg acgatctgca aagaaacgga gttcaaaatg
     3181 tgatgtccgt ccggcgcctg gacgctgaag gagtccaggg agacgatcca gcggaatttt
     3241 tctgaaacgg cggcggcgcc ggcggtcttt tcctgaaacg gcgccgtcat gggttcctcg
     3301 aaaacggacg tttttcccca aagaccagga tgtcgtcctt ggcgcttttg atgtcgagca
     3361 cgggagtgcc gtcgatggcg tcgagccctt gcacgcgcag gacgccgccg cggcaactca
     3421 gcagtttcac gcgcgtcatg gccaggggat tgggccggtc cggggaatgc gtggaaaaga
     3481 ccccgcgcaa ctttttgtct ttcggaaatc gccgtcgcct tgcccgcagg acgtcccggt
     3541 ccgctttgtg gaaataacac aggacccaaa gggaaggaga cgccgtaatg cgataaaggg
     3601 cttttcggaa ctttcggcga aggacgattt ccgccgtcgt tccctggttc ggcatgtgcc
     3661 ggcgtgtttt gacggcggag cggacgctcc cgatgggctc cagaatgacc cgcacttaaa
     3721 aagtcatttc cagtcccgtc agataggtcc ttccgggcat gggatcgtag aagttcagcg
     3781 catattcctg gtcggtgatg ttgttgaagc gaagataata ggtgaagtgg cgggcaaagg
     3841 tccatccgcc gctccaatga tacaccgtgt aggcggcccg ttttccggta aagttgtcca
     3901 gggtgtaccg cccgccggcg tgcgtcatgt cgaccgtggt cgagaagcgg ccgtcccggt
     3961 aggtcagact ggactgtgcg gtgttcttgt ccgtgtggaa ggtgagcgtg cccgggtcca
     4021 ggtaggagta ccccgcgccc gcctcgaaat gcgacaggaa ggcgtagcgc gcccggacct
     4081 ccacgccgcg ataggccgat ttgccgtcca cgttgcggta ctgtttttgc actgcggcgc
     4141 cctgataggt cagcggcgcg acggggacct gggagacgat catgtcggtc tccacgttct
     4201 tgtacgccga gaggtcgatc ccgcccgctt ttttagaata gcttaatccg atctcgtgct
     4261 ggcggttctt atccgggcgg aggtgggcgt tgccgaacgc ccgggtgttg gtggaataca
     4321 gctcggtcac cgtcggcgcg cggaagccgt agttgaggtt ggcacgggcg cgcaggtccg
     4381 gcaggatctt ccacacgacg ccgtatttcg gggtccactg gtccgtgaac ttggagttgt
     4441 tgtcgtagcg gacaccggcc aggaatatga aacgctcatg ccagacgggg agggacagtt
     4501 gaatgaacgg cgccacgtac tgctgcgttg cgcgcgaact cggcgaaccg gatcggatgt
     4561 gggaatcctc gtagtccgtg ccgaaaaaga gttccgcgcg cagcggcaac tccccctgcc
     4621 actcgaggct gccgccctgc gtgctggccc gggacaacgg cgagctggcg gaccggtcac
     4681 ggtcggtcgt caggaaagtt cggacataaa agcgcgaccg gccgaaagtc ctctgccagg
     4741 tcacatagga gttcgtgatc tcctcctcga tgtccttgtc gatgcccgtg aacgcccccg
     4801 cgttggttga gaagacggct tcattccgtt tcgtgttgaa gttgc
//
LOCUS       DAOTFD010000201         4842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12704_length_4842_cov_3.548987, whole genome shotgun sequence.
ACCESSION   DAOTFD010000201 DAOTFD010000000
VERSION     DAOTFD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12704_length_4842_cov_3.548987"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4842
                     /locus_tag="P5079_09675"
     CDS             <1..>4842
                     /locus_tag="P5079_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30289.1"
                     /translation="VRAGTGGWVTPPPSYKGGYGYGYGYGDSDYYEPDPEDLEPYFEG
                     ATVTTLYAIDGSGHLVQLPGGSWGMVNGTTVTQADADRINELVDVAGVPLQGLTLVAV
                     PKGEGEDAEMTMQVMGILPVTRVVLERILNAYRGDLEAALGLPDGVHIDEVELTNDGE
                     TIVIHLTNGETISVPYSWLNGGYQEPDYGYNDDYGYGYGYGYGGPPVDLGPNYQQIFG
                     SLSWRAQIALSVFMMLGSADRPGASGDLEENTFLDLSGTLLIGASGVSGTFDIKYRDK
                     ATGRITKVGTMQIGILGKGLTYAATWYMGVDVVRRAIAKGDLKLPNVDPSVDPKDIPG
                     YKPATTTGTTHRAPKYDREGNLIDPGEEGEDEEPVTTPEVIPPQPPLPTDNPPVNEGD
                     EGWPAGGTHVQGTYTVGIMNGAKMQMAQWTIYDAQGRKLQEYTRGYQEGKGYSATDRI
                     YTGWDPNTGRATSYNELGWSQGNGFYRETVTNITYDSQGRIFSYHSEKINNFDDPIIT
                     DRLETYYDQAGRVTRTEEVKSWGNNGDVPGMGSDYINGRNVHVTTTYRYNAAGQMVGM
                     YAHASGGSVTVKTTDKNGKPNGQRYFYNPNVSYSQTNIRYDRYGRIVSYDESSSSNCQ
                     WDAQNQRFKSGGNSGHTVNYSFDQFGRVLSSRSDYRNSDGSRGYNISTNRVYNSLGQL
                     LSEHSTYRSETRRTVTTGSKDTRYYYVNGRFDRTQDFNKHEKTKTKGFWGSIFGKIIN
                     MVINIVGSILMKTGVFSWLGAIILFVWNFFYTYMTTGSFKLALIAGVMAVISGLIGAS
                     GGMNLLQKVALNFVVGFLGTYLATGDWRAALGAGVGAAVGTALAGIMPAGTTTQVVMA
                     AIVGGLVGFAVGYATARLFGADSRMALLAGIYAGVTAAGSVSKSSGVAGGAGDAAKDV
                     AKDVAKEVAKEATKEVAKMTAKQFMQEFVKQFVSQLIGLVVSEVLPDDLKFMAGAITA
                     AISAVVGKIIVANTSAGSSANNLGLALGGGQTWQQVLGGVAGQAARALAGYFIGGALG
                     RDNEDSSKDYSYLTAALISLVDGLDILTKGGPNAFGNYVKFVMGGIIRSVKDYYMKLA
                     KDVFPEIGLNAAVYDLLGTSLSSFDVLADKFQSSAVSKGLGNFIANTFGKLIVGLAMT
                     IKAVFVEIRKAVPDGKGGFVKGDRIFWFVGQNVRIWNDAGEGRGVVTDMMGTLVADKV
                     FAKDGSIATDASGKALKPFFNETTGNWEAMTLAQRNEKFHSIVPTSAESGSEEAAQAD
                     AAEEAGASGAIAAAQAEATEPTASGDAATETADIPDAEPTLMEMNVSENQTVIEAMTA
                     FLDSMRGNISDADFEYLSEVLSSGVELAKACTFNVTGFAVSIDANGNVVSASVKWDKT
                     LSDGTKVTGAFTTDFKTHQRTTVINGNLTFNQRKEMLGYLVAGKVITAAQAKIISKLW
                     ESNAQKANNCGFNITGFSFTQDAGGKLLYVSTLFAANVTVNNKAYVVEGAFKTDMKGN
                     TITEFKLDTPELKGLTSELRSQLRGALLDGLVRGKVISADQKIKIEQQWAAKDAMLQK
                     AGCRVVALNLKQDSSGGLLSTGIEFRKTVTTGSGGKLTISGSVTTEMSGRTTWSFGTE
                     MSQL"
BASE COUNT         1020 a         1421 c         1523 g          878 t
ORIGIN      
        1 gtccgggccg gcaccggcgg ctgggtgacg cccccgccgt cctataaggg cggatacggg
       61 tatggttacg gttacgggga ctccgattat tatgagccgg atccggagga tctggagccc
      121 tatttcgaag gcgccacggt taccaccctt tacgccatcg acgggtccgg ccatctcgtc
      181 caactgcccg gcggcagctg gggcatggtc aacgggacga ccgtgaccca ggcggacgct
      241 gaccgcatca acgagttggt ggacgtggcc ggcgtgccgc tgcagggcct caccctcgtg
      301 gccgttccga aaggcgaagg ggaggacgcc gagatgacca tgcaggtgat gggcatcctg
      361 ccggtcaccc gcgtcgttct ggagcggata ctcaacgcct accgaggcga cctggaggcc
      421 gccctcggcc tgcccgacgg cgtccatatc gacgaggtgg aactgaccaa tgacggggaa
      481 accatcgtca tccatctcac caacggcgag accatctccg tcccctactc ttggctgaac
      541 ggcggctatc aggaacccga ctatggatac aacgacgatt atgggtacgg ctatggttac
      601 gggtatggcg gtccgccggt cgacctcggt cccaactatc agcagatttt cgggagtctg
      661 tcctggcggg cccagatcgc cctgtccgtc ttcatgatgc tcggttccgc cgaccggccc
      721 ggggcgtccg gcgacttgga agagaacacc ttcctcgacc tcagcggcac tctcctcatc
      781 ggcgccagcg gcgtttccgg gactttcgat atcaagtatc gggacaaggc caccggcagg
      841 ataaccaaag tcggcaccat gcagatcggc atcctcggca aaggattgac ctatgccgcc
      901 acgtggtata tgggcgtgga cgtcgtccgc cgggccatcg ccaagggcga tctgaaactg
      961 ccgaatgtgg atccctccgt ggatccgaaa gacattccgg ggtacaaacc cgccaccacc
     1021 accggcacca cccatcgcgc gccgaaatac gaccgggaag gcaacctgat cgatccgggc
     1081 gaggaaggag aggacgagga acccgtcacg acccctgagg tcatcccgcc ccagccgccg
     1141 cttcccaccg acaatccgcc cgtgaacgaa ggcgacgagg gttggccggc gggcggcacg
     1201 cacgtgcagg gcacctacac cgtggggatc atgaacggtg ccaagatgca gatggcgcag
     1261 tggaccattt acgacgccca ggggcggaaa ctgcaggaat acacccgcgg ttaccaggag
     1321 ggcaagggct actccgccac ggaccgcatt tacaccggct gggatcccaa caccggccgc
     1381 gccacttcct ataacgagct cggctggagc caaggcaacg ggttctatcg cgagaccgtc
     1441 accaacatca cctacgacag ccaaggccgc atcttctcct atcattccga aaagatcaac
     1501 aacttcgacg accccatcat caccgaccgc ctcgagacgt actacgatca ggccggccgg
     1561 gtcacccgca cggaggaggt caagagctgg gggaacaacg gcgacgtgcc gggcatgggc
     1621 agcgattaca tcaacggtcg caacgtccac gtcaccacca cctatcggta caacgccgcc
     1681 ggccagatgg tgggcatgta tgctcacgcc agcggcggtt cggtgacggt caagaccacg
     1741 gacaagaacg gaaagccgaa cgggcagcgt tacttctata atccgaacgt ttcctattct
     1801 cagaccaaca tccgttacga ccgttacggc cggatcgtct cttacgacga gtcctcctcc
     1861 agcaactgcc agtgggacgc gcagaaccag cggttcaaga gcggtggcaa ctcgggccat
     1921 accgtcaact actctttcga ccaattcggc cgcgtcctgt cgagccggtc cgactaccgt
     1981 aactccgacg gcagccgggg ctacaacatc tccaccaacc gggtctacaa cagtctcggc
     2041 cagttgctct ccgaacattc cacctatcgc agtgaaaccc gccggacggt gaccacgggc
     2101 tccaaggaca cccggtatta ttacgtcaac ggccgttttg accgcaccca ggactttaac
     2161 aagcacgaaa agacgaagac caagggtttc tggggttcca tcttcggcaa gatcatcaac
     2221 atggtcatca acatcgtggg cagcatcctg atgaaaaccg gggtgttctc gtggctcggg
     2281 gccatcatcc tctttgtgtg gaacttcttc tatacctaca tgacgacggg gagtttcaag
     2341 ctggcgctga tcgccggtgt gatggccgtt atctcgggcc tcatcggggc gagcggtggg
     2401 atgaacctcc tccaaaaggt cgcgctcaat ttcgttgtgg gattcctcgg cacctatctt
     2461 gccaccggcg attggcgggc cgccttaggg gccggggtgg gggctgccgt cggcacggcg
     2521 ctcgccggca tcatgccggc cggcaccacg actcaggtgg tgatggccgc catcgtgggg
     2581 ggattggtgg gttttgccgt tggttacgcc accgcccgct tgtttggcgc ggacagtcgt
     2641 atggctctcc tcgccggaat ctatgcgggg gtgacggcgg cgggtagtgt ttccaagtcc
     2701 agcggcgttg cgggcggggc cggagacgcg gctaaagatg tggccaagga cgtggctaaa
     2761 gaggtggcca aggaggcgac aaaagaggtc gccaagatga ccgccaagca gttcatgcag
     2821 gaattcgtga aacagttcgt ttcgcagctc atcggcctgg tcgtttccga ggtgttgcct
     2881 gacgatctga aattcatggc gggcgcgatc accgcggcga tcagcgccgt ggtgggcaag
     2941 atcatcgtgg ccaatacttc cgccggcagc tccgccaata acctggggtt ggccctcggc
     3001 ggcggacaga catggcaaca ggtccttggc ggcgtggccg gccaggcggc gcgcgccttg
     3061 gcgggttatt tcatcggcgg cgcgttgggt cgggacaatg aggactccag caaagattat
     3121 tcctatctga cggccgcgtt gataagcctg gtggatggtt tggatattct gaccaagggc
     3181 gggccgaacg ctttcgggaa ttacgtcaag ttcgtgatgg gcgggatcat acgttccgtc
     3241 aaggactact acatgaagct cgccaaggac gtctttccgg agatcggttt gaacgcggcg
     3301 gtttacgacc tcctgggcac ttcgctgagt tcgtttgatg tcctcgccga taaattccaa
     3361 tcgtccgccg tctccaaggg gctggggaac ttcatcgcga acacgttcgg taaactcatc
     3421 gtgggcctgg cgatgacgat caaggctgtt ttcgtcgaaa tacggaaagc cgtgccggat
     3481 gggaaaggcg gattcgtgaa aggcgatcgg attttctggt tcgtcggcca gaacgttcgg
     3541 atttggaacg acgccggcga agggcgtggc gtggtaacgg acatgatggg gacgctcgtc
     3601 gcggacaagg tttttgcgaa agacggttcg atcgccacgg atgcctcggg taaggcatta
     3661 aagccgttct ttaatgagac cacggggaac tgggaggcca tgacgttggc gcaaaggaac
     3721 gagaagttcc attccatcgt tcctacgtcg gcagagtcgg gatcggaaga ggccgcccag
     3781 gcggatgccg cggaagaagc cggcgcctcc ggggccatcg cggcggccca ggccgaggcc
     3841 accgagccga cggcctccgg ggatgcggcg actgagacgg cggacattcc ggatgcggaa
     3901 ccgacgttga tggaaatgaa tgtttccgaa aaccagacgg tgatcgaggc gatgacggcg
     3961 ttcctggaca gtatgcgggg gaacatctcg gatgcggatt ttgagtatct gtcggaggtt
     4021 ttgagctccg gcgtcgagct tgcgaaggcg tgcacattta acgtgaccgg gttcgccgtc
     4081 tccatcgatg ccaacgggaa tgtggtgtcg gcctcggtca aatgggacaa gacgttgagt
     4141 gacgggacca aggtgacggg cgcgttcacc acggatttca agacccatca gaggaccacg
     4201 gtcatcaacg gcaacctgac cttcaatcag cgcaaggaga tgctgggcta cctcgtcgcc
     4261 gggaaagtga tcacggcggc ccaggccaaa atcatcagca agttgtggga gagcaacgcc
     4321 cagaaagcca acaactgcgg attcaacatc acgggctttt cctttacgca ggacgccggc
     4381 ggcaagttgc tctatgtgtc cacgttgttc gcggccaacg ttaccgtgaa caacaaggcc
     4441 tatgtggtgg agggggcctt caagacggac atgaaaggca ataccatcac ggaattcaag
     4501 ctggacactc cggaactgaa aggactcacc tcggaattgc ggtcgcagct ccgaggggcc
     4561 ctcctggacg gtctggtcag aggcaaggtc atcagcgcgg accagaaaat aaaaatcgaa
     4621 cagcagtggg ccgccaagga tgcgatgctg cagaaggcgg ggtgtcgggt ggtggccctc
     4681 aaccttaaac aggattcttc gggcgggctg ctcagcaccg gcatcgagtt ccggaaaacg
     4741 gttaccacgg gcagcggcgg caaactgacc atttccggtt ccgtgactac cgaaatgagc
     4801 ggccgcacca cctggagttt cggcaccgag atgagccagc tc
//
LOCUS       DAOTFD010000202         4831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12741_length_4831_cov_4.867044, whole genome shotgun sequence.
ACCESSION   DAOTFD010000202 DAOTFD010000000
VERSION     DAOTFD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_4831_cov_4.867044"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="P5079_09680"
     CDS             <1..961
                     /locus_tag="P5079_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30290.1"
                     /translation="SGLSPMVNPMPDRRTVPPYKCLYTWDVHYACNYRCSYCFFNSSW
                     EEEKKKNRYPGIARWKNIWDAVYERYGGGLIHVSGGEPFTYPDFIDLLVHLRKKFDTE
                     HDTNLSFDLKEFMEKVAPERVKLNATFHPQFADIETFFDKALTLKKAGFALGVNYVAF
                     PEQLGRMAEYKKRFNDAKISFDIMPFRGTYQGREYPQGYDESEKKILCEAAPKTAPPM
                     FAAYDQKKSPAPSNPHAGKLCRMGQMYTKIQPDGTAYRCCKAEPAGKLGNLIDGTFSF
                     YEHPQPCGYTGCSCWRAMIVDQEQNWLRCWLSPKEIERGKPGQ"
     gene            1016..1975
                     /locus_tag="P5079_09685"
     CDS             1016..1975
                     /locus_tag="P5079_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRY30291.1"
                     /translation="MFNKLCDRFSGLMMVWVVLACGAGYVWPPVLVWMKPHLDWLFML
                     TMLGIGIVTPPHDFNEIVKRPRLVALGMAAQFGIMPVCAFLVAKGLSLPKDLAVGLIL
                     AGAAPDAMAAGVVSYAAHADVAYSVALTGTSTLLAPVVTPALTYFFGKTYIEIAFTPM
                     MISILKMVIAPLAVGMAIKRATGKRLDGMVALFPALSTVFIAGICGLVVALNKDTLAE
                     VTGLVFVAVFLLNLLGLALGYGAGMLFGFDVKRRRTLAIGVGMQNAGLGAVLAIKHIS
                     ARAAVPNALFATWCIVSASLLAWYWSRRDRRTNAEDVPRSTVV"
     gene            2032..2382
                     /locus_tag="P5079_09690"
     CDS             2032..2382
                     /locus_tag="P5079_09690"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRY30292.1"
                     /translation="MNPLAEIKCVPCREGAEPVTLAEIDEFLPFIPDWQIIERAGIPR
                     LERAFEFKDFAEAMAFTDRIAALAEQEGHHPVLITEWGRVTVAWWTHKIKGLHRNDFI
                     MAAKTDERLKTPAA"
     gene            2474..2662
                     /locus_tag="P5079_09695"
     CDS             2474..2662
                     /locus_tag="P5079_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30293.1"
                     /translation="MKTLKLSLAALVAFMLSGCVLTKVVTVPMRVTGAAISIIPVAGN
                     AVDGAIDKAADGVDKLPF"
     gene            2784..3128
                     /locus_tag="P5079_09700"
     CDS             2784..3128
                     /locus_tag="P5079_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRY30294.1"
                     /translation="MEIAADANRVAFCGLYCGACGRYLKGACPGCQQNAKAAWCAVRT
                     CCLESHFSTCVDCKTYTEPNDCKKFNNFVSKIVGFVLRSDRRACLQQIREKGLAAHAA
                     DRTAAKQPSIRR"
     gene            3133..3408
                     /locus_tag="P5079_09705"
     CDS             3133..3408
                     /locus_tag="P5079_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30295.1"
                     /translation="MPRPTAKSRHPADGGDGPEDIYASQREELLSRLTACVRQNQPLT
                     AYESPVLSSDEAAVVLQKIKNSHPFSRKERRGLLEAGFAVDKLYPRL"
     gene            3425..4603
                     /locus_tag="P5079_09710"
     CDS             3425..4603
                     /locus_tag="P5079_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY30296.1"
                     /translation="MSLLSKQQIESRLADYFGRKFCCLTNRGTTALTAALHALGKPAG
                     ATTLFPAVMCSIPVFSAKFAGGRPEFADVDLTDGNFSLPDVDRVLAERAGEVGAVVPV
                     HMFGQPEKMEELQTLCDKHGAALIEDGALSMGARHKGRPVGRFGAMSCLSFVRKMLPL
                     EMGGAVLTDDTLLNKRVRAFVEDLPPPPPNQRERTEAAMKEFHTLTGRVAANGWKDTA
                     LFRPYEKTFAELFLSRTVEEDWKDSVVLDELAALEDVVKARRARAEVYETVLTHPRLP
                     SLEHGESCFFAYPVRLTDVGVESFLEFADAEGFSYRRIAYPDVHKVFGSSRPDGAFPN
                     ARILEKELMGLAVDDDQPVSSFWEYAQDFLKIFERYLEEGTWRAPDSWEGKLERRMGT
                     "
     gene            4633..>4831
                     /locus_tag="P5079_09715"
     CDS             4633..>4831
                     /locus_tag="P5079_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30297.1"
                     /translation="MKIPYRTLIVLAVVGGGVGFLYYRSTVEEKPAAKQSFGEGGGRG
                     SGGAGGQQGAAGDGQQAGGQDG"
BASE COUNT          938 a         1524 c         1490 g          879 t
ORIGIN      
        1 gagcggctta tctccgatgg tgaacccgat gccggaccga cggaccgtcc caccgtataa
       61 atgcctgtac acctgggacg tccactatgc ctgcaactac cgctgttcct actgtttttt
      121 caattcctcc tgggaagaag agaaaaaaaa gaaccgctac cccggcatcg cccgttggaa
      181 aaacatctgg gacgccgttt acgagcgcta cgggggagga ctcatccacg tttcgggcgg
      241 agaaccgttc acctacccgg acttcatcga cctgctcgtc cacctcagga aaaaattcga
      301 caccgaacac gacaccaacc tgtccttcga cctgaaggaa ttcatggaga aagtcgcccc
      361 ggaacgggtg aaactgaacg cgaccttcca tccccagttc gcggacatcg aaacgttctt
      421 cgacaaggcc ctgacgctga aaaaagcggg ctttgccctg ggggtgaact acgtggcttt
      481 tccggaacag ctcgggcgga tggcggaata caaaaaaagg ttcaacgacg cgaaaatctc
      541 gttcgacatc atgccgttcc gggggacgta tcagggcagg gaatatcccc aggggtacga
      601 cgaatcggag aaaaaaatcc tgtgcgaagc cgcccccaaa accgccccgc ccatgttcgc
      661 cgcctacgat caaaagaaat ccccggcgcc gtccaatccg cacgcgggga aactgtgccg
      721 catggggcag atgtacacca agatccagcc ggacggcacg gcctaccggt gctgcaaagc
      781 ggaacccgcc gggaaactgg gcaacctcat cgacggcacg ttttccttct atgaacaccc
      841 ccaaccctgc ggctacacgg gctgttcctg ctggcgggcg atgatcgtcg accaggaaca
      901 gaactggctg cgctgctggc tctctcccaa agagatcgaa cgggggaaac caggacaatg
      961 accggcggac ttcgggattg tccgcggcga aaatttttga aaagaggaat gttccatgtt
     1021 caataagctg tgcgaccgtt tttccgggct gatgatggtg tgggtcgtcc tggcgtgcgg
     1081 agccggctac gtctggccgc ccgtgctcgt ttggatgaaa ccccatctgg attggctgtt
     1141 catgctgacc atgctgggga tcggcatcgt gacgccgccg cacgatttca acgagatcgt
     1201 caaacgccct cgcctcgtgg cgctggggat ggcggcccag ttcggcatca tgccggtgtg
     1261 cgcgttcctg gtggccaaag gactgtcgct gccgaaagac ctggccgtcg gcctcatcct
     1321 ggcgggggcc gcgccggacg ccatggccgc cggcgtggtg agttacgccg cccacgcgga
     1381 cgtggcctat tccgtggcgc tcaccggcac ctccaccctg ttggcgcccg tcgtgacgcc
     1441 cgccctcacg tattttttcg gaaaaacata catcgagatc gcgttcaccc ccatgatgat
     1501 cagcatcctg aaaatggtca tcgcgcccct ggccgtcggc atggccatca agcgcgccac
     1561 cgggaaacgc ttggacggca tggtcgcgct cttcccggcc ttgtcgacgg tgttcatcgc
     1621 ggggatatgc ggcctggtgg tggcgttgaa caaagacact ctggcggagg tcaccgggct
     1681 cgttttcgtc gcggtgttcc tgctgaacct gctgggactg gcgctgggtt acggggcggg
     1741 gatgttgttc ggctttgacg tgaagcgccg ccgcaccctg gccatcgggg tggggatgca
     1801 gaacgcggga ctgggcgcgg tgctggccat caaacacatc agcgcgcggg cggccgtgcc
     1861 caacgccctg ttcgccacct ggtgcatcgt atcggcctcg ttgttggcct ggtattggtc
     1921 ccgccgcgat cgccggacga acgccgagga cgtcccacgt tccaccgttg tttgagccgt
     1981 ccgggaaccc gaaaggggaa aaaatcgtta tagaccgtga ggaggcggaa catgaacccc
     2041 cttgcggaaa tcaaatgcgt gccctgccgg gaaggcgccg aacccgtgac cttggcggaa
     2101 atcgacgagt tccttccgtt catccccgac tggcagatca tcgaacgggc cggcatcccc
     2161 cgcttggaac gcgcttttga gttcaaggat tttgccgaag cgatggcttt caccgaccgg
     2221 atcgccgccc tggccgagca ggagggccat cacccggtcc tgatcacgga gtggggacgg
     2281 gtgaccgtcg cgtggtggac gcataagatc aagggattgc accgcaacga tttcatcatg
     2341 gccgccaaaa ccgacgagcg gctgaagacg cccgccgcct gagatcgtcg ttccgttttg
     2401 cctcttcccc ggcgaaccaa accccatcca agttggtata atagccgcaa taaaatcggc
     2461 aaaggagttt ttcatgaaaa cgctcaaact gtcgttggcg gcgctggtcg ccttcatgct
     2521 gtccggttgc gtcctcacca aagtggtgac ggtccccatg cgcgtgacgg gcgcggccat
     2581 ctccatcatt cccgtggcgg gcaacgccgt ggatggggcg atcgacaaag ccgccgacgg
     2641 cgtggacaag cttcctttct aaacgccttt cgaccgggtc cgcggcgccg tcgagggacg
     2701 gacccgcggc ggccccgtgc ggcatactcc cccggcccat ggccggagga gtatgatttt
     2761 tgcctgaaag tggggaaaac tctatggaaa tcgcggcgga cgcgaaccgg gtggccttct
     2821 gcggcctcta ctgcggggct tgtggacgtt atttaaaagg cgcgtgcccc ggatgccaac
     2881 agaacgccaa agccgcctgg tgcgccgtgc ggacatgctg tctggaatcc catttctcca
     2941 cctgcgtgga ctgcaagacc tacaccgaac cgaacgactg taaaaagttc aacaacttcg
     3001 tctcgaaaat cgtcggtttc gtcttgcgct ccgaccgccg cgcctgcctc cagcagatcc
     3061 gcgaaaaagg cctggccgcc cacgccgccg atagaacggc cgccaaacag ccgtcgatcc
     3121 ggcgctgacc ccgtgccgcg tcccacggcc aagtcgaggc accccgccga cggcggcgac
     3181 ggcccggaag acatctacgc ctcccaacgg gaggaattgc tctcccgcct caccgcctgc
     3241 gtgcgccaga accagccgct gacggcctac gagtcgccgg tgctctcctc cgatgaagcc
     3301 gcggtggtcc tgcaaaaaat aaagaacagc cacccctttt cccgcaagga acgccgcggt
     3361 ctcctggagg ccgggttcgc cgtggacaag ctttaccccc gtctctgaat ccgttcgctc
     3421 ccgaatgtct ctcctttcaa aacaacagat cgagtcccgc ctggccgatt atttcggccg
     3481 gaaattctgc tgcctcacca accggggcac cacggcgctg accgcggccc tgcacgccct
     3541 ggggaaaccg gccggcgcca cgacgctgtt ccccgcggtg atgtgttcca tcccggtgtt
     3601 ttcggccaaa ttcgccggtg gtcggccgga gttcgccgac gtggacctga ccgacggaaa
     3661 tttttccctg ccggacgtgg accgcgttct ggcggaacgc gccggagagg tcggggcggt
     3721 ggtccccgtc cacatgttcg gccagccgga gaagatggaa gagctgcaga cgctctgcga
     3781 caaacacgga gcggccttga tcgaggacgg agcgctgtcc atgggcgcgc gccacaaggg
     3841 ccggccggtg ggacggttcg gcgcgatgtc gtgcctgagt ttcgtccgga aaatgctgcc
     3901 gctggaaatg ggcggcgcgg tgctgaccga cgacaccctc ttgaacaaac gggtccgggc
     3961 cttcgtcgag gacctgcccc cgccgccgcc gaaccagcgg gaacggacgg aggcggccat
     4021 gaaagaattc cataccctca ccggccgcgt ggcggccaac ggctggaagg acaccgccct
     4081 ttttcggccc tatgagaaga ccttcgccga actgttcctt tcccgcaccg tcgaggaaga
     4141 ctggaaagac tcggtggtgt tggacgaact ggcggcgctg gaggatgtgg tcaaggcccg
     4201 gcgggcgcgg gcggaggtct acgagaccgt gctgacgcat ccccgcctgc cgtcgctgga
     4261 acacggggaa agctgctttt tcgcctaccc ggtgcggctg acggacgtcg gcgtggaaag
     4321 ttttctggag ttcgccgacg ccgaagggtt ctcctatcgc cgcatcgcct atccggacgt
     4381 gcacaaggtg ttcggttcgt cccggccgga cggcgccttc cccaacgcgc gcatcctgga
     4441 aaaagaactc atgggcctgg ccgtggacga cgaccagccg gtctcgtctt tttgggaata
     4501 cgcccaggat ttcctgaaaa tcttcgaacg ctacctcgaa gaggggacgt ggcgcgcgcc
     4561 ggattcctgg gaaggcaaac tcgaacgccg catggggaca tgattccttt tttttgatac
     4621 acttctaaag ttatgaagat cccttatcgc actctcatcg tcctggctgt cgtcggtggc
     4681 ggcgtgggtt ttctctatta ccgcagcacg gtggaggaaa aacccgcggc caaacagtcg
     4741 ttcggcgaag gcggcggacg gggctccggc ggcgcgggag gccaacaggg cgccgcgggc
     4801 gacgggcaac aggccggcgg acaggacgga c
//
LOCUS       DAOTFD010000203         4811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_12825_length_4811_cov_3.891085, whole genome shotgun sequence.
ACCESSION   DAOTFD010000203 DAOTFD010000000
VERSION     DAOTFD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_4811_cov_3.891085"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="P5079_09720"
     CDS             complement(<1..954)
                     /locus_tag="P5079_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30298.1"
                     /translation="MSELFDLRARRPSPQRLSDMLVSAGTAGVPIAVHIALCLHHLGQ
                     RDYFSFEGANGITIFNWLWGWAKTPHLVLEGCGFPLLPLITYLTSLVLSHRNAVPAFA
                     VTEIAVSAVGILLFFLICRSLGFSRIISMLITLAAIPHVENIYNGCVTNYNDNFFCTS
                     TLMAVLGWLSYRQSNRSRYLYIAATGALFASMSRWEGFIIPATVTLLLFFSDPGARRR
                     TRAASFTFFSAVSLSWLYVGLMAVYVLFSNRWGDPLGGQKDGNAYIFQSGVLSPISIF
                     LSELFHAPKNIVLLGAAGAMAFWHAVRSPKSSLSPLWNQYLL"
     gene            992..>2231
                     /locus_tag="P5079_09725"
     CDS             992..>2231
                     /locus_tag="P5079_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HRY30299.1"
                     /translation="MNILGISAYAHESSACLLKEGRPVALVEEERFNRERHTAKFPHE
                     AIRAVLEMGEIEAHAIDGITFFWKPWKEIVENVGHLLRFFPASLHLLAVPSGSEEATA
                     AGRLIRMMGLRRPLSEHGALAEKPVYYVEHHLCHAASAFFVSPFEEAAILTLDGRGER
                     ATTVLSVGRGARLDKVWQQNVPHSLGHLYAAVTSHLGFRPFCDEWKVMALAAHATPAG
                     RDSFVDLLRLNEDGGFELDLRYFSFHVHGRGRWVSSAFIAKYGPPRLPEGKIEQRHCD
                     LAGAVQDAVEKAGLHLARRLRQVSKSPRLCLAGGVALNCLMNKALVERGPFREVFIQP
                     LANDAGTSLGSALYHHHQILGRPRTFVWEDVYLGPAYSEGEMEQALQAAGLGYRKSSS
                     VAAEAARRVAEGKIVGWFQGR"
     assembly_gap    2232..2241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2242..2732
                     /locus_tag="P5079_09730"
     CDS             <2242..2732
                     /locus_tag="P5079_09730"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRY30300.1"
                     /translation="GRMEAGPRALGNRSILADARRSRSRDILNKVIKKREWFRPFAPA
                     VLAEKADMFFNLKGPSPHMMLAADMKEEKKGVIPAVVHADGTARVQTVTKSQNPLFWE
                     LLVELDKLTGVPVVLNTSFNENEPIVCSPEQAIDCFRRSGMDLLVLGNCIVERTPALP
                     PA"
     gene            2759..>4811
                     /gene="carB"
                     /locus_tag="P5079_09735"
     CDS             2759..>4811
                     /gene="carB"
                     /locus_tag="P5079_09735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRY30301.1"
                     /translation="MPKRTDIKTILIIGSGPIVIGQACEFDYSGAQAVKVLKSEGYRV
                     VLINSNPATIMTDPEFAHATYVEPLVPSMIERIIEKEKPQVLLPTLGGQTALNLAVEL
                     AERGSLKKYNVELIGANLEAIRRAEDRELFKKTMLAIGLDVPRSGVARSLDEAGKVAG
                     EIGFPLIIRPSFTLGGVGASIVFRREDFSAAVSRGLDASPIHEVLLEESVIGWKEFEL
                     EVMRDRADQCVVICSIENLDPMGVHTGDSITVAPAQTLSDPDFQRMRDQSFAVLRAIG
                     VETGGSNVQFALHPKTGRLVVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     IPNDITKKTPACFEPVMDYVVTKVPRFAFEKFTEGDQTLGPSMKSVGEVMAIGRTFRE
                     SLQKAVRGLEIGRAGLGADGKGQVRRVEEIRDERRRGRSSSDTEAYLEEVRNKLRLPN
                     CDRIFNIKYALQLGLSVEEIHRLSQIDPWFIEQIRGLVVLEDEIRRAGKRISSELLRR
                     AKRNGFSDDQIAYLTGWSSDQLRRRREKLKIAPTYKKVDTCAAEFPADTPYFYSTYEE
                     EDESLPPSKKKRVMVLGGGPNRIGQGIEFDYCCVHAAWALGEAGYETVMVNCNPETVS
                     TDYDTSDQLFFEPLTGEDVLNIVRRVKPAGVIVQFGGQTPLNLAKPLLKAGVKILGTS
                     AD"
BASE COUNT          984 a         1280 c         1624 g          913 t
ORIGIN      
        1 aaggagatat tgattccata atggagagag ggaggatttc ggagaacgca ccgcgtgcca
       61 gaacgccatc gccccggcgg cgccgagcag gacgatattt tttggggcgt gaaaaagttc
      121 ggagaggaag atggagatgg ggctgaggac gccggattgg aaaatgtagg cgtttccgtc
      181 tttttggcca ccgagagggt ctccccagcg gtttgaaaaa aggacataga cggccatgag
      241 gcccacatag agccaggaaa gggagacggc ggagaaaaat gtgaaagaag cggcccgggt
      301 tctccgccgg gcgccgggat cggaaaaaaa caggaggagg gtgacggtgg cggggatgat
      361 gaaaccttcc catcgcgaca tggaggcgaa cagggctccc gtggctgcga tataaagata
      421 gcgggaccgg ttgctctggc ggtaactcag ccagcccagc accgccatca gcgtcgaggt
      481 gcagaagaag ttgtcgttgt aattcgtgac gcaaccgtta tagatgtttt ccacgtgagg
      541 gatggcggcc agtgtaatga gcattgagat aatgcgtgaa aatccgagag aacggcagat
      601 aaggaaaaac aaaaggatgc cgacggcgct caccgcgatt tccgtaacag caaaggcagg
      661 gacggcgttt ctgtgtgaaa ggaccaaaga agtgagatag gtgatcaagg gcaggagtgg
      721 aaatccgcag ccttcgagaa cgagatgggg ggtctttgcc cagccccaca gccaattgaa
      781 gatggtgatg ccgttggccc cttcaaacga aaaatagtct cgttgaccca ggtgatgcaa
      841 gcagagggcg atgtggacgg cgatgggaac gcccgcggtc ccggctgata cgagcatgtc
      901 ggagaggcgc tgaggagagg gccggcgcgc gcggaggtcg aaaagttcag acatggaggg
      961 ggcggtcggt ggatcggggt cttggggtga gatgaatatt ctgggaatca gcgcctacgc
     1021 gcatgaatct tccgcttgcc tgctgaagga ggggcggccg gtcgcgctcg tggaggagga
     1081 acggttcaat cgggaacggc atacggccaa atttccgcac gaagcgatcc gagcggtcct
     1141 ggaaatgggg gaaatcgaag cccatgcgat agacggtata acattttttt ggaaaccgtg
     1201 gaaagaaatc gtcgaaaacg tcgggcactt gctgcggttc tttccggcga gcctgcatct
     1261 gttggcggtg ccttccgggt cggaagaggc gacggcggcc ggccggttga ttcggatgat
     1321 ggggttgcgc cggcccttgt cggaacatgg ggcgttggcg gagaagccgg tctattacgt
     1381 cgaacatcat ctttgtcatg cggccagcgc gtttttcgtt tcgccgttcg aagaggcggc
     1441 gatccttacc ttggacggcc gtggagagcg ggcgaccact gttttatccg tcggacgtgg
     1501 cgcgcgtctg gataaggtgt ggcagcaaaa cgtgccgcat tcgttggggc atctgtacgc
     1561 cgccgtcacc tcccatctgg ggttccggcc tttttgtgac gagtggaagg tgatggcgtt
     1621 ggccgcccac gccactccgg ccggccggga ctctttcgtc gaccttctcc ggttgaacga
     1681 agacggtgga tttgagttgg acctgcgata tttttctttt catgtccatg gccgtggccg
     1741 gtgggtctct tcggccttca tcgcgaaata cggccccccg cgtcttccgg agggaaagat
     1801 cgagcagcga cactgcgatt tggccggcgc cgttcaagac gccgtcgaaa aggcggggtt
     1861 gcatctggcg cgccgcctcc gccaagtgtc aaagagcccg cgtctctgtc tggccggcgg
     1921 cgtggcgttg aattgtctga tgaacaaggc gctcgtggag cgggggccgt tcagggaagt
     1981 tttcatccag ccgttggcca acgatgccgg gacatcgttg gggagcgctt tataccatca
     2041 tcatcagatc ctgggccggc caaggacgtt cgtgtgggag gatgtctatc tcggcccggc
     2101 ctacagcgaa ggggaaatgg agcaggcgct tcaggccgcc gggctcggtt atcgaaaatc
     2161 atcgtcggtc gcggcggagg cggcgcggcg ggtggcggaa gggaagatcg tgggatggtt
     2221 ccagggacgc annnnnnnnn nagggacgca tggaggccgg ccctcgggct ttggggaacc
     2281 gcagcatcct ggccgacgcc cgtcggtcgc ggagccggga tatcctcaat aaggtgatca
     2341 agaaacggga atggttccgg cctttcgccc cggcggtgtt ggcggagaag gcggacatgt
     2401 tctttaatct gaagggcccg tcgcctcata tgatgctggc ggcggacatg aaggaagaga
     2461 aaaaaggggt gatcccggcg gtggtgcatg ccgacgggac ggcccgggtt cagacggtga
     2521 cgaaatccca gaacccgctc ttttgggaac tccttgtcga attggacaag ttgaccggcg
     2581 tgcccgtggt gttgaacacg tcgttcaatg agaacgagcc catcgtctgt tcgccggaac
     2641 aggccatcga ttgtttccgg cgttcgggga tggacctgct tgtgctgggc aactgcatcg
     2701 tcgaaagaac gccggcgctc ccgccggcgt aattgaattc caaaggggac gaagtcatat
     2761 gcctaaacgg acagacataa agaccatcct catcatcggc tccggcccca tcgtcatcgg
     2821 acaggcctgc gagttcgact attccggcgc ccaggccgtc aaggtgctga agtccgaagg
     2881 ataccgcgtc gttttgatca actccaaccc ggccaccatc atgacggatc ccgagttcgc
     2941 ccacgccacc tacgtggagc cgctcgtccc gtccatgatc gagcgcatca ttgagaagga
     3001 aaagccgcag gtgctcctgc ccacgctggg tggccagacc gcgcttaacc tggctgtgga
     3061 gctggcggag cgcggatctc tgaagaaata caacgtggag ttgatcgggg ccaatttgga
     3121 ggccatccgt cgggccgagg accgggaact cttcaagaag accatgctcg ccatcgggct
     3181 ggacgtgccg cggagcggcg tggcgcgttc cttggacgag gcgggaaaag tggccggcga
     3241 gatcggtttt ccgctcatca ttcggccgtc cttcactctg ggcggcgtgg gggcgtccat
     3301 cgtctttcgg cgagaggatt tttccgccgc ggtgtcgcgc ggcctggacg ccagccccat
     3361 ccatgaggtc ctcctggagg aatcggtcat cgggtggaag gagttcgagt tggaggtgat
     3421 gcgcgaccgg gcggatcaat gcgtggtgat ctgttccatc gagaacctgg atcccatggg
     3481 cgtgcatacc ggcgattcca tcacggtggc tccggcgcag accctgtccg acccggattt
     3541 tcagcggatg cgcgaccaat ctttcgccgt cctgcgcgcc atcggggtgg agacgggcgg
     3601 gtccaacgtt cagttcgccc tccacccgaa gaccggtcgg ctggtggtga tcgagatgaa
     3661 cccgcgcgtt tcgcgttcct cggcgctggc ctccaaggcc acgggatttc ccatcgcgaa
     3721 gatcgcggcg ttgttggcgg tggggtaccg gctggacgag atccccaacg acatcaccaa
     3781 gaaaacgccg gcctgtttcg aaccggtgat ggattacgtg gtgacgaagg tgccgcgttt
     3841 cgctttcgag aaatttaccg agggggacca gaccctcggt cccagcatga agtcggtggg
     3901 cgaggtgatg gccatcggcc gcacgttccg cgagtccctc caaaaggcgg tgcgggggct
     3961 ggaaatcggg cgggccgggc tgggagccga cggaaagggc caagtgcggc gcgtggaaga
     4021 aatacgcgac gaacggcgtc gcggtcgatc gtcctccgat accgaggcct acctggaaga
     4081 ggtgcgcaac aaactgcgcc tgcccaactg cgaccggatt ttcaacatca aatacgccct
     4141 gcaactcggt ttgtccgttg aggagatcca ccggctttcc cagatcgatc catggttcat
     4201 cgaacaaatc cgcggattgg tggttctgga ggacgaaatc cgccgggccg ggaaaaggat
     4261 atcgtccgaa ctccttcggc gggccaagcg gaacggcttt tccgacgatc agatcgccta
     4321 tctgaccggc tggtcttcgg atcagctccg ccgccgtcga gaaaaactta aaatcgcgcc
     4381 gacgtacaag aaggtggata cctgcgccgc ggagttcccg gcggacaccc cctattttta
     4441 ttccacctat gaggaggagg acgagtccct gccgccgtcg aagaaaaaac gggtgatggt
     4501 tctgggcggc gggccgaacc gtatcggaca ggggattgaa ttcgattatt gctgcgtcca
     4561 cgcggcttgg gcgctcgggg aggcggggta cgaaacggtg atggtgaact gcaaccccga
     4621 aacggtttcc accgattacg acacctccga ccagctcttt tttgagccgc tcacggggga
     4681 agacgtgctg aacatcgtgc gccgcgtgaa gccggcgggc gtcatcgttc aattcggcgg
     4741 acagacgccg ttgaacctgg cgaaaccgtt gttgaaggcg ggcgtgaaga tcctcggcac
     4801 atcggcggac t
//
LOCUS       DAOTFD010000204         4750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_13059_length_4750_cov_4.068584, whole genome shotgun sequence.
ACCESSION   DAOTFD010000204 DAOTFD010000000
VERSION     DAOTFD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13059_length_4750_cov_4.068584"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5079_09740"
     CDS             complement(<1..572)
                     /locus_tag="P5079_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30302.1"
                     /translation="MTNPTPLPESPLAALFSRLVAYFLPVTYFLITVSFYLKTYDSAQ
                     IKITLTQIGCTAVILFWFCQLVLERRWPFEKKDLPLIAPFLAFFTSGVVSFLQSSFQQ
                     GSVEEFIRRVLYCFMGVIVISDFRGWDRHRRLLRWLLAAFGVVVFYGFVQYFDTRLFP
                     AGQGVGIDPFVWRYAFGLRVFSSFGNPNFYG"
     gene            complement(598..1944)
                     /locus_tag="P5079_09745"
     CDS             complement(598..1944)
                     /locus_tag="P5079_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRY30303.1"
                     /translation="MIDVSAGVAGKSGFDLLKISPFPRSAAMAEATTAVVGDPSALYS
                     NPAGLAHTSMSQISAQHAAHLEDVSIDNLAMIFPYARGGWGLDLGFSGVKDIAKTKYD
                     PSSVDRFVETGEMKAGDQFVTVSWGRRQTSNLALGVSVKAVQEKLDTETAMSVMGDVG
                     GIYRFDRQWRGAVVVQNMGSSGKFISEAVPAPLRGRAAVHWTPEEWMFWEVDGVMNGD
                     GSSEALLGGEFHWEQMAFFRLGYRYPFQNNDLGIATGFSVGVGFQAGPFGADYAFLPY
                     GDLGDSHRFAVSWRFGNAVSVSDEPPPDRPFSAGGAASTVKKIVAVADFEPKNVSSMD
                     ASITADFLRSELAREGAFAVVSRPDMDRLLAGENVQMSGCTTKECAVRMAKILNADKV
                     VMGSVALIKKKYSITANIVDVEAGFTEYSTTVGELALDQVPPAARDIAQKLAAAYQ"
     gene            complement(2161..2232)
                     /locus_tag="P5079_09750"
     tRNA            complement(2161..2232)
                     /locus_tag="P5079_09750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Thr,seq:cgt)
     gene            complement(2443..4188)
                     /locus_tag="P5079_09755"
     CDS             complement(2443..4188)
                     /locus_tag="P5079_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY30304.1"
                     /translation="MVKAVINSIPVEVPENTSILDAAKTVGVNIPILCKHPDLHASAA
                     CGICVVKMKGSQKMPRACCTPIEEGMDIVTHDPEIVSVRKTVLNLILSNHPNSCLTCG
                     RNTQCELQTALADFGIREEPYDKLLCSAPLDSTTGTIVLDPPKCIKCGRCVTVCQEMQ
                     NVWALSFLERGIETRISPAGDISLGDSPCVRCGQCSAHCPTGAIVEKDDTEKVWAALQ
                     NPAKHCVVQIAPAVRVTLGEHFGFEPGVNLSKKIYTLLRRMGFERVFDTNFGADLTIM
                     EEAAEFVQRFVHGKSVLPLITTCCPSWVDFMEKMHPDMIEHFSSSKSPHEMLGVLAKT
                     YYAEKQGLSPADIEVVSVMPCTAKKFEIHRSREMSASGFQDVDTSVTTRELSRMIRQA
                     GIDFANLPDGECDPPLGDYSGAGTLFGTTGGVMEAALRTAAFMITKEKLPRVEFENVR
                     GLKGVRETSVTVAGKEVRIAVAHGLANVEYVIEKIRKAKKDGAPLPYHFVEVMACPGG
                     CIGGGGQPYGVTDELRAKRARGLYSDDKNLPRRCSYENPYIQQLYEDYLGEPLSKKAH
                     ELLHTTYKPRPMYQR"
     gene            complement(4198..>4750)
                     /locus_tag="P5079_09760"
     CDS             complement(4198..>4750)
                     /locus_tag="P5079_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRY30305.1"
                     /translation="KVFAVTGKVKNSGLVEVPMGTTLRDIVYAICGGAAGGKKVKAVQ
                     TGGPSGGVIPEQYFDTPVGYENLQSLGSIMGSGGMIVMDEDDSMVDITKFYLGFCVDE
                     SCGKCAPCRVGGTQMLRILNRISAGDGEPADLEKLRDISLAMQKASLCGLGQTAPNPV
                     LSTLRYFEPEYRALIKTPASSGK"
BASE COUNT          972 a         1534 c         1366 g          878 t
ORIGIN      
        1 ccgtaaaagt tcgggttgcc gaaagaagag aacacgcgca acccgaaagc ataccgccag
       61 acgaacggat cgatccccac gccctgcccc gccgggaaga gccgggtgtc gaaatactgc
      121 acaaacccgt agaacaccac cacgccgaac gccgccaaca gccaccgcaa caggcggcgg
      181 tgccggtccc agccgcggaa atccgaaatg acgatcacgc ccatgaaaca gtacagcacc
      241 cggcggatga actcctccac gctgccctgc tggaaggagg actgcaggaa ggagacgacc
      301 ccggaagtga agaacgccag gaacggagcg atcagcggca ggtccttctt ctcgaaaggc
      361 cagcgccgct ccagcacgag ctggcagaac cagaagagga tcaccgccgt gcagccgatc
      421 tgcgtgagag tgatcttgat ctgggcagag tcgtaagtct tcaggtaaaa ggagaccgtg
      481 atgaggaaat acgtcacggg caggaagtaa gccaccaggc gggaaaaaag cgcggccagc
      541 ggcgattcgg gaaggggcgt cgggttcgtc atcgggactt tccggtcggc ggctgaatta
      601 ttgataggcc gccgccagct tttgggcgat gtcccgcgcg gccgggggga cctggtcgag
      661 agccaactct ccgaccgtgg tcgagtattc cgtgaaaccg gcctccacgt ccacgatgtt
      721 cgccgtgatg gaatatttct tttttatcag ggccaccgac cccatgacca ccttgtccgc
      781 gttcaaaatc tttgccatcc gcaccgcgca ttctttcgtg gtgcatcccg acatctggac
      841 gttctctccg gcgaggagcc ggtccatgtc cggacggctc accaccgcga aagcaccctc
      901 ccgcgccaac tccgaacgca ggaaatccgc cgtgatggag gcgtccatcg acgaaacgtt
      961 tttcggttcg aaatcggcca ccgccacgat cttctttacc gtcgacgccg cgcccccggc
     1021 cgaaaacgga cgatcgggcg gcggctcatc cgacaccgac accgcgttcc cgaacctcca
     1081 cgacaccgca aaccggtgag aatcccccaa atcgccgtag ggcaaaaaag catagtccgc
     1141 gccgaacggc ccggcctgga accccacgcc gaccgaaaaa ccggtggcga tgcccaaatc
     1201 gttgttctgg aacggataac ggtatcccag acggaagaac gccatctgtt cccagtggaa
     1261 ctccccgccc aacagcgcct cggaactgcc gtccccgttc atcacgccgt ccacttccca
     1321 aaacatccat tcctccggcg tccagtggac cgcggcccgc ccccgaagcg gcgccggcac
     1381 cgcttcggaa atgaacttcc ccgaagaacc catgttctga accaccaccg cccctcgcca
     1441 ttgacggtcg aaacggtaaa tcccccccac gtcgcccatc acggacatcg ccgtctccgt
     1501 atcgagcttt tcctgcaccg ccttcacgga cacgcccagc gccaggttgg acgtttgccg
     1561 ccgcccccaa gaaaccgtca cgaactggtc cccggccttc atttccccgg tttcgacgaa
     1621 acgatccacc gacgacgggt cgtatttcgt tttggcgatg tccttcacgc cggaaaaacc
     1681 cagatccaat ccccaaccgc cgcgcgcata gggaaaaatc atcgccaaat tgtcgatgga
     1741 aacgtcttcc aagtgcgccg cgtgctgggc cgagatctgg gacatcgagg tatgcgccag
     1801 accggcgggg ttggaataga gggccgacgg gtcccccacc acggccgtgg tggcctcggc
     1861 catggccgcc gaacgcggga aaggcgatat cttcaacaga tcaaatccgc ttttccccgc
     1921 cacccccgcg gagacatcga tcacaccgaa aaaaaacaca gcccacgcca tagttactag
     1981 gcggggcggt cgatcggatc gggcgtgtgt ggaaaagttc gtcatttctt tctgttccgg
     2041 gaaatcgttg cgccagaacc ggtggcgcgc ggatcggctt caatccccag tggagttttc
     2101 atccctccgg gatgccgttg gtcccaaagc catgcgcggg acaatcatca aaaaaatcta
     2161 agccgaggat ggggattgaa cccacaacct atcgcttacg aagcgattgc tctaccgttg
     2221 agctacctcg gcgcaaaact tcgttttgtg gcaaacgtcg atccttgcgc atggtggctg
     2281 ttttcgttac aaacaaatgg gactgggcgg tcaatggaac gccgctgctg aaacgcgccc
     2341 cattgtatca gttttttgaa attgcccgcc aattagttcg ccgaacgaag atcagaccgc
     2401 cggccggaat ccggcggagc cgaaaacccg gccttccagg ccctaccgct gatacatggg
     2461 gcggggctta taggtcgtgt gcaacaactc gtgcgccttt ttgctcaacg gttcgcccag
     2521 atagtcctcg tagagttgtt ggatgtacgg attttcatag gaacagcgtc tcggcaaatt
     2581 tttatcgtcc gaataaagcc cacgggcccg cttggcgcgc agttcatcgg tgacgccgta
     2641 cggctgcccg cccccgccga tgcatccgcc cggacacgcc atcacctcca cgaaatggta
     2701 ggggagcggg gcgccgtcct ttttggcctt gcgtatcttt tcaataacgt attccacatt
     2761 ggcgaggccg tgggcgacgg cgatgcgcac ctccttgccc gccaccgtca ccgaggtttc
     2821 cctcacgccc ttgagccccc gcacgttttc gaactccacc cggggcagct tctctttcgt
     2881 gatcatgaaa gcggcggtgc gcaacgccgc ctccatcacg ccgccggtcg ttccgaagag
     2941 cgtcccggcg ccgctgtagt cgcccaaagg cgggtcgcat tcgccgtcgg gcaggttggc
     3001 gaagtcgatc cccgcctggc ggatcatgcg ggacaattcc cgggtggtga cggaggtgtc
     3061 cacgtcctgg aaccccgaag cgctcatttc ccggctccga tggatctcga acttcttcgc
     3121 cgtgcacggc atgacggaca ccacttcgat gtcggcggga gagagtccct gtttctcggc
     3181 gtagtaggtc ttggccaaaa cccccagcat ctcgtgcggc gacttgctcg acgaaaaatg
     3241 ttcgatcatg tccgggtgca tcttttccat gaagtccacc caggaagggc aacaggtcgt
     3301 gatgagcggc aggacggact tcccatggac gaacctctgc acgaactccg cggcctcctc
     3361 catgatggtc aggtcggcgc cgaagttggt gtcgaacacc cgctcgaacc ccatgcgccg
     3421 gagcaaggta tagattttct tcgacaggtt gacgccgggt tcgaacccga agtgttcccc
     3481 cagcgtcacc cgcaccgccg gagcgatctg cacgacgcag tgcttggcgg ggttctgcag
     3541 ggcggcccac accttctccg tgtcgtcctt ttcgacgatg gcgccggtcg ggcaatgggc
     3601 agaacactgg ccgcagcgca cgcagggaga atcccccaga gaaatgtcgc cggccggcga
     3661 aatgcgggtc tcgatccccc gctccaggaa cgaaagagcc cagacgttct gcatctcctg
     3721 gcacaccgtg acgcaacggc cgcacttgat gcatttcggt ggatccagga cgatggtgcc
     3781 ggtggtggaa tccagggggg ccgagcagag gagcttgtcg taaggctcct cgcgtatgcc
     3841 gaaatccgcc agggccgttt gaagttcgca ctgggtattg cggccgcagg tgaggcagga
     3901 gttgggatgg ttcgacagga tcaggttcag caccgttttt ctgacggaga cgatctcggg
     3961 atcgtgcgtg acgatgtcca tcccctcttc gatcggcgtg cagcaggcgc gcggcatttt
     4021 ctgagaaccc ttcatcttga ccacacagat accgcacgcg gccgaggcgt gaaggtcggg
     4081 gtgcttgcag aggatcggga tattgacccc cacggtcttc gcggcgtcga ggatggaggt
     4141 gttctccggg acttccaccg ggatcgaatt gatgacggct ttgaccataa aattccctta
     4201 tttcccggac gaagcgggcg tcttgatcaa cgcacggtat tccggctcga aataccgcag
     4261 ggtcgacaga acggggttcg gggccgtttg ccccaatccg cacagggacg ccttctgcat
     4321 ggcgagggag atgtcccgga gtttctccag atcggccggt tccccgtcgc cggcggagat
     4381 ccggttcaat atccgcaaca tctgcgtgcc gccgacgcgg cagggcgcgc acttgccgca
     4441 ggactcgtcc acacagaacc ccaggtagaa cttggtgatg tccaccatgg agtcgtcctc
     4501 gtccatcacg atcatcccgc cggaccccat gatggaaccc agggattgca ggttctcgta
     4561 gccgacgggg gtgtcgaaat attgttccgg gatgaccccg ccggacggcc cccccgtttg
     4621 gaccgctttc acttttttac cgccggccgc tccaccgcaa atcgcgtaca cgatgtcccg
     4681 caaggtcgtc cccatcggga cctccaccag ccccgagttc ttgactttgc ccgtgacggc
     4741 gaacaccttg
//
LOCUS       DAOTFD010000205         4736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_13113_length_4736_cov_3.461440, whole genome shotgun sequence.
ACCESSION   DAOTFD010000205 DAOTFD010000000
VERSION     DAOTFD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13113_length_4736_cov_3.461440"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="holA"
                     /locus_tag="P5079_09765"
     CDS             <1..1032
                     /gene="holA"
                     /locus_tag="P5079_09765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRY30306.1"
                     /translation="GDSSGLECALAHWARGAFKPVYLFAGEDTLRKEETLKKFQGHFF
                     GKSSPEMNTDIFEGGTAAAGHILTAVRTFSLLGDRRLVLVKRAQDMPAAEINPLADGI
                     PSLSGPNCLVLLLDEKAKETSVLVQAVKSAGSVMTFWMPFDNQLPAWVQERARSLGKS
                     ISTETAKTLVENVGPSLPDLLQELEKLSLQLKGKSTIDMADVESIQSDTRVLQYMELD
                     RALWRRDRAKVLSLLETLRHQGQKSEGFPPRLAGIYRKLFLAKALISEKNSPDDVCNQ
                     FRIKSWQLKNEFLEALRSYTWDELLGCMEAVLEAERDFKTGRMEGDSGMTLLICRLTK
                     TEEEAGLLR"
     gene            complement(1021..1305)
                     /gene="rpsT"
                     /locus_tag="P5079_09770"
     CDS             complement(1021..1305)
                     /gene="rpsT"
                     /locus_tag="P5079_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY30307.1"
                     /translation="MAKLKTGRHTSALKVARQAVKHRWRNATAKDGAKELTKELLAAV
                     SKKDAAKAKELLPQVMSVWSRIGRRNVVHHAMASRKIARLSKAVLRISAK"
     gene            1364..2923
                     /gene="murJ"
                     /locus_tag="P5079_09775"
     CDS             1364..2923
                     /gene="murJ"
                     /locus_tag="P5079_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRY30308.1"
                     /translation="MNSSHTLIRHVGAVSFATLISRILGYVRDALVAYAFGGGRFTDA
                     FYAAFRLPNLFRRLLGEGSLTAAFVPIFSEHISNDRQDAARECFQALFTLLLTVLFSI
                     VVLGMFFAPQVTHLVAWGFKSDPDLFDLTVRLTRQIFPFLLFVSLAALASGSLNSLGH
                     FFVPSVAPAMLSVAEITFVLLIAKSCSDPLQGLAVSAVAGGLLHFGLLVPLLYKEKMP
                     LVWRWNPLHPDIRRVGKALLPAVWGMSLDQVNAFIDTICASFLITGSVTALYNSNRLM
                     QFPLALFGIAMSTAALPNLSASAAKQDWPAMKNTLNLALRLILFAVLPAMAGLILLGR
                     PAIELLFEHGKFTHRETLLTNAALAMYCLGLPAFAGVKVLASFFYALKDTKTPVKVAN
                     ACLLINITGNLTLMWKFGVAGLAIATSLASTANAFILFHLLRRRLGLMGGRRIFKTFL
                     QCAAALFPMMLAAGGIAYGLTGSLIWRVPAAVLVGTAAYLGTAKLLRMEEFQHLRNAL
                     TGSPDPRETPQ"
     gene            2937..4295
                     /locus_tag="P5079_09780"
     CDS             2937..4295
                     /locus_tag="P5079_09780"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRY30309.1"
                     /translation="MKSHPSLLQQLPHSPGVYLMRNSAGAILYIGKAVDLRKRVSNYF
                     RPTGLDVKIRALMAEVKHIDYIAAGNEREALVIEQRLINRHQPAYNTMWRDDKSYPYV
                     RISLNEDFPRIDLTRRKKNDGARYFGPFPDVRPIRRLLQWIWNRHIFPLRPCRLAFSE
                     NQWPPRSKVQSCIYLHTGKCPAPCVGRISRKDYRKIAERVVLFFEGGHARLEKTWRRE
                     MKAAAKARNFELAAHLRDNIRAVEQIRQRVTVRALRPEEVQSRIEATQALSELKNALA
                     LPRPPLRIEAFDISHSQGTATVASLVTFDRGKPAKAGYRKYIIKTVRGVDDFASMEEV
                     VGRRYRRLMEEEAAMPDLILVDGGPGQLSAALKALEKAGVKKQPIAALAKQEEEIYRP
                     GAPEPVRLPKDSPALQLLQHVRDESHRFAVGFHRQRRGKGMWGDGNRLPGRQSAGPAG
                     NV"
     gene            4327..>4736
                     /locus_tag="P5079_09785"
     CDS             4327..>4736
                     /locus_tag="P5079_09785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRY30310.1"
                     /translation="MKIPKLLQKRMASYPRFYQAVWRACAQIPRGETRTYGWIAKKIG
                     IPRAARAVGMALAANPFAPVIPCHRVIRADGSLGGYSGAGGLKKKRRMLAKESSGRLR
                     FNRTGKILTALLLASLAASTAVGAAKPPQPRPNV"
BASE COUNT          899 a         1583 c         1368 g          886 t
ORIGIN      
        1 ggcgattcct ccggccttga atgcgccctc gcgcactggg cccgaggggc cttcaagccc
       61 gtctacctct tcgccggcga ggacaccctc cggaaagaag aaaccctcaa gaaattccag
      121 ggacattttt tcggcaagag ttcgccggag atgaacaccg acatttttga aggcgggacc
      181 gccgcggccg gacacatcct caccgcggtc agaaccttct ccctgctggg ggaccggcgg
      241 ctggtgctgg tgaaacgggc ccaggacatg cccgccgccg agatcaaccc gctggcggac
      301 ggcatcccct ccctttccgg tcccaactgt ctcgtgctcc tcctggatga gaaggcgaaa
      361 gagaccagcg tgctggtgca ggccgtgaag tcggcgggga gcgtcatgac cttctggatg
      421 ccgttcgaca accagctgcc cgcctgggtg caggaacgcg cgcgatcgct ggggaaatcc
      481 atctccacgg aaacggccaa gaccttggtg gaaaacgtcg ggcccagcct tccggatctg
      541 ctccaggaac tggaaaagct ctctctccag ctgaaaggga aatccacgat tgacatggcc
      601 gatgtggaat ccatccaatc ggacacgcgg gtcttgcaat acatggaact cgaccgggcc
      661 ctctggcggc gggaccgggc caaagtgctc tcccttttgg aaaccttgcg acaccagggg
      721 cagaaatccg aaggttttcc gccgcggctg gccggcatct accggaaact tttcctcgcc
      781 aaagccctga tctccgagaa aaactcaccg gacgacgtct gcaaccagtt ccgtatcaaa
      841 tcctggcagt tgaaaaatga attcctggag gccctgcgga gttacacgtg ggacgaactg
      901 ctgggctgca tggaggcggt gctggaagcg gaaagggatt tcaaaacagg ccggatggaa
      961 ggggactccg ggatgacgct gctgatctgt cggttgacga aaacggaaga agaggcgggg
     1021 ttacttcgct gaaatgcgga ggacggcctt ggagagccgg gcgatcttgc gcgacgccat
     1081 ggcgtgatgg acgacgttgc ggcggccgat gcgggaccac acggacatga cctggggcag
     1141 aagttccttc gctttcgccg cgtccttttt ggacacggcc gcgagcaact ccttggtcag
     1201 ttccttggcg ccgtctttgg cggtcgcgtt ccgccagcgg tgtttcaccg cctgacgcgc
     1261 cactttgagc gccgatgtat gacgaccggt tttcagttta gccataagag agcataataa
     1321 tacttgtgcg cctgttaaaa gtcaaggaaa ttcaaatttc gacatgaatt cttcccatac
     1381 gctcatccgg cacgtcgggg ccgtctcgtt cgccacgctg atatcccgca tcctcgggta
     1441 cgtccgcgat gcgctggtgg cctatgcgtt cggcggggga cggttcacgg acgccttcta
     1501 cgccgccttc cggctcccga acctgttccg ccgtttgctg ggggaagggt cgctcacggc
     1561 ggccttcgtg cccatcttct ccgaacacat ttccaacgac cgccaggacg ccgcgcggga
     1621 gtgtttccag gccctgttca ccctgcttct gaccgtcctc ttctccatcg tcgtcctggg
     1681 aatgttcttc gcgccccagg tgacccacct cgtggcctgg gggttcaagt ccgaccccga
     1741 cctgttcgat ctgaccgtcc gcctcacccg ccagatattt cctttccttc tgttcgtgtc
     1801 cctcgccgcg ctggcctccg gctccctcaa ttccctgggg catttcttcg tcccttccgt
     1861 ggctcccgcc atgctgtcgg tggcggagat cacctttgtt ctcctcatcg cgaaatcctg
     1921 ttcggacccg ctccaaggtc ttgccgtcag cgccgtggcg gggggactcc tccacttcgg
     1981 actcctcgtg ccgctcctct acaaggaaaa gatgcccctg gtgtggcgtt ggaacccgct
     2041 gcatccggac atccgccgcg tggggaaagc cctcctgccc gccgtctggg gaatgtcgct
     2101 cgaccaggtg aacgcgttca tcgacaccat ctgcgcctcg ttcctcatca ccggttccgt
     2161 caccgcgctt tacaactcca accggctgat gcagttccct ctggcgctgt tcggcatcgc
     2221 catgtccacc gcggccctgc ccaacctttc cgccagcgcg gcgaaacagg actggccggc
     2281 catgaagaac accctcaacc tcgccctgcg gctgatcctt ttcgccgtcc ttcccgccat
     2341 ggccggactg attctcctcg gccgacccgc catcgaactc cttttcgagc acggaaaatt
     2401 cacccaccgg gaaaccctcc tcaccaacgc ggcgttggcc atgtattgtc tgggccttcc
     2461 cgcctttgcc ggcgtgaaag tgctggccag ctttttctac gctctcaagg acaccaagac
     2521 gccggtgaaa gtggccaacg cctgtctttt gatcaacatc accgggaacc tcaccctcat
     2581 gtggaaattc ggcgtggcgg gactggcaat cgccaccagc ctggcctcca ccgccaacgc
     2641 cttcatcctc tttcacctgc tgcggcgacg gctgggattg atgggcggcc ggcggatctt
     2701 caaaacattc ctgcaatgcg cggcggccct gttcccgatg atgctggcgg cgggagggat
     2761 cgcctacgga ttgaccggca gtctgatttg gcgcgtgccc gcggccgtgc tcgtggggac
     2821 ggccgcctat ctgggaacgg caaaactcct ccgtatggaa gaattccagc acctgcgaaa
     2881 cgccctcacg gggtcccccg accctcggga gacgccgcaa taacggtcga tcccccttga
     2941 aatcccaccc ctccctgctt caacaactgc cgcactcgcc gggtgtgtat ctcatgcgga
     3001 attccgccgg agccatcctt tacatcggca aggccgtcga cctgcgcaaa cgagtatcaa
     3061 actatttccg tcccaccggc ctggacgtga agatacgggc cctgatggcc gaagtcaaac
     3121 acatcgacta catcgccgcg gggaacgaac gcgaggccct ggtgatcgaa cagcgcctca
     3181 tcaaccgcca ccaacccgcc tacaacacca tgtggcgcga cgacaaaagc tatccctacg
     3241 tccgaatatc tttgaacgag gattttccgc gcatcgacct tacgcgccgc aaaaaaaacg
     3301 acggcgcccg gtatttcggc cccttccccg acgtgcgtcc gatccgccgc cttctgcagt
     3361 ggatatggaa ccggcacatc ttcccgttgc ggccgtgtcg gctggcgttt tcggaaaacc
     3421 aatggccgcc gcgttcaaaa gtccaatcct gcatctatct ccacaccggc aaatgtccgg
     3481 ccccctgcgt gggacggatt tcccggaaag actaccgaaa aatcgccgag cgcgtggtgc
     3541 tcttcttcga gggggggcac gcccgcctgg aaaagacctg gcggcgggag atgaaggccg
     3601 cggccaaggc gcggaatttc gaattggcgg cgcacctgcg cgacaacatt cgcgccgtcg
     3661 aacagatccg ccagcgcgtc accgtccgcg ccctgcgtcc ggaagaggtt cagagccgca
     3721 tcgaggccac ccaggccctc tcggaattga aaaacgccct ggccctcccc cggccgcccc
     3781 tgcggatcga agctttcgac atctcccatt cgcagggcac ggcgaccgtg gcttccctgg
     3841 tgacgttcga ccgggggaaa ccggcgaagg ccggataccg gaaatacatc atcaagaccg
     3901 tgcgcggcgt ggacgatttc gcgtccatgg aggaagtggt gggccggcga tatcgtcgcc
     3961 tgatggagga agaagcggcg atgcccgatc tgatcctggt cgacggcggg ccgggccaac
     4021 tgtcggcggc gttgaaagcc ctggaaaagg cgggggtgaa aaaacagccg atcgcggcgc
     4081 tcgccaaaca ggaagaagag atctaccgac cgggcgcgcc ggaaccggtc cggctcccca
     4141 aggattcacc cgccctgcag cttctccagc acgtgcgcga cgagtcccat cgcttcgccg
     4201 tgggcttcca tcgccaacgg cgcggaaaag gaatgtgggg agatggaaac agactccccg
     4261 gccggcaaag cgcgggaccg gcaggcaacg tttgaacggc gccggcgacg gcgcccaaga
     4321 gggctcatga aaattccgaa acttctccaa aagcgaatgg cttcctatcc tcggttttac
     4381 caagcggtgt ggcgggcctg cgcccaaatc ccgcgcgggg aaacgcggac ctacggatgg
     4441 atcgccaaaa aaataggcat cccgcgggcc gcccgcgccg tggggatggc cttggccgcc
     4501 aacccgttcg ctcctgtcat cccctgccac cgcgtcatac gcgccgacgg ctccctcggc
     4561 ggctattccg gggcgggggg attaaaaaag aaacgccgga tgctcgccaa agaatcctcc
     4621 gggcgtcttc gtttcaaccg gacaggcaaa attttaacgg cgctcctcct cgcctccctc
     4681 gccgcctcga ctgccgtcgg cgccgccaaa ccgccgcaac ctcgccccaa cgtcat
//
LOCUS       DAOTFD010000206         4684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_13316_length_4684_cov_3.536401, whole genome shotgun sequence.
ACCESSION   DAOTFD010000206 DAOTFD010000000
VERSION     DAOTFD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13316_length_4684_cov_3.536401"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="P5079_09790"
     CDS             complement(<1..1290)
                     /locus_tag="P5079_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY30311.1"
                     /translation="MKVKVSDVIADFLVKKGMKHTFGIIGSGNAHIFDSIYRTTAIQM
                     VCVHHEQAACMALQTYYRTSGTVTAAVLTTGAGSTNGITGVVSAWADSIPGMIIVSNE
                     NSKFTRPESPLRMWGVQGYDSVDMVKKVTKYAVRVADPLKILYELEKTYALAVSGRPG
                     PCWIEIPMNVQSAVVEDSELTRFDPREIEIRRDMVQLQKLADRVRDEISKSSRPLVWL
                     GYGIRIAGALDLLKEMLEKVRLPYLISWAGVDMVDSDHPLLYGRAGVYGQRAANFVLQ
                     NCDYLLTIGTRLAIPQVGYDISELARDAKIAVVDVDQSELDKYKERFDLPICADAGDF
                     LRCFMKSVSAKPVPSPTEWLSTCTGYRQKYPWVGPEHADKDGYINSYRFMEKLDRHFK
                     PDQVVTTDMGTALLCAFQVLRIHDGQRLMTSTGLGEMG"
     gene            complement(1304..2368)
                     /locus_tag="P5079_09795"
     CDS             complement(1304..2368)
                     /locus_tag="P5079_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012264577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY30312.1"
                     /translation="MSPKTSNTLLASDLNQVLSEIGDDWRDLKNGRLFLTGGTGFFGC
                     WLLESFLWANEHLGLKAKVVVLTRAPRAFRNRWPHLANNPSLSFLRGDVRKFKFPKGA
                     FSHVIHAATDASAQLNDENPLQMLDVIVEGTRRVLEFSHKTGVKKFLYVSSGAVYGDQ
                     PESVPHIAETYRGAPDAASPRSAYGEGKRLGETLCSIFSKDTSLQTKVARCFAFVGPY
                     QPLDRHFAIGNFIGNVLRGEPIRLKGDGSPVRSYLYGADLATWLWKILFRGEKCRPYN
                     VGSKNRVTIKELAHKIADVLGPGIPVEFAKQSSPGIAPDQYVPSTKRAQSELGLRERV
                     SLDEAIRRTAKWHRDLYQTK"
     gene            complement(2379..3344)
                     /locus_tag="P5079_09800"
     CDS             complement(2379..3344)
                     /locus_tag="P5079_09800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30313.1"
                     /translation="MSEPATKKLISVVIPAYNEEGNIEELGRRLQGVFQKNPKYDFEA
                     IIVENGSHDSTFEKLKELHEKDKRFKIVLLSRNFKCDGGITAGLQHASGDAAVVMTAD
                     LQDPPELITTFIQKWEEGYENVYGVITKRHGSSLLRRFNSQLFYILVNKLTGGLFPRN
                     ASDFRLVDKRVYRAVNSMWERNRFLRGMFIWTGFKSIGVEHERAPRFAGEAKSHTLVV
                     LRLAVMGILSYSYVPLKAITAVGVFVSTLSFLILVFIVIRVFAWGVPFPGFGTIMAVM
                     LLMFGFLFTMLGIMSEYIGLIYEEVKQRPNFIVKEAVGFDNLKKS"
     gene            complement(3369..>4684)
                     /gene="rfbH"
                     /locus_tag="P5079_09805"
     CDS             complement(3369..>4684)
                     /gene="rfbH"
                     /locus_tag="P5079_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HRY30314.1"
                     /translation="AASAENREAVYREVREFFKRSHGPKKFVPGESPVPVSGKVYDAD
                     DMLHLIDASLDFWLTTGRFGDEFEKKLAKRLGLSYSVLVNSGSSANLLAVSALTSPTL
                     KDRQLKPGDEVVTLAASFPTTVNPLIQNRLVPVFIDVELGTYGPRPERLAEAVSPKTR
                     AIMIAHTLGNPFELDAVVHLAQKHNLWLIEDSCDALGSLYKGKPVGTFGQLATFSFYP
                     AHHITMGEGGAVATANNRLHSILESVRDWGRDCWCAPGKDNTCGKRFDWQLGDLPDGY
                     DHKYVYSHVGYNLKATDMQAAVGVSQLNKLDDFIRRRRHNFDLLHKGLSKYSEFLILP
                     EPTPGSEPAWFGFPITVKSTAPFRREDLTRHLENNKIGTRLLFAGNIVRQPYFKGIEY
                     RTVGDLKNTDQVMNGTFWIGVYPGITDGMITYMLEQFESFFKKSA"
BASE COUNT         1014 a         1212 c         1418 g         1040 t
ORIGIN      
        1 gcccatttcc cccagccccg tggacgtcat gagccgttgt ccgtcatgta ttcgcagaac
       61 ctgaaacgcg cataaaagcg ccgttcccat gtccgtcgtg acaacctggt ccggcttaaa
      121 atgccggtcg agcttttcca tgaatctata ggagttgata tagccgtcct tgtcggcatg
      181 ttccggcccg acccaggggt atttttggcg ataaccggtg cacgtggaca gccattccgt
      241 cggagacgga acgggtttgg cggacaccga tttcatgaag caccgcaaaa agtcgccggc
      301 gtccgcgcag atcggcagat caaatcgttc cttatacttg tccaattccg actggtccac
      361 gtccacgact gcgatcttcg cgtcccgcgc caattcggag atatcgtagc cgacctgcgg
      421 gatggccagg cgtgtgccta tggtgagaag ataatcgcaa ttctgcaaca cgaaattcgc
      481 agcacgctgg ccatagaccc ccgcccggcc gtacaggagg gggtggtccg aatccaccat
      541 atcaacgcct gcccatgaga tgagataagg aaggcgaact ttctccagca tttccttcaa
      601 caggtccagg gcccctgcga tgcgaattcc gtatcccagc caaacgagtg gccgggacga
      661 ctttgagatt tcatccctga cgcgatccgc cagtttttgc aattggacca tgtcccgacg
      721 gatctctatt tcgcggggat cgaaccgagt caattcggaa tcttccacca cggcggattg
      781 cacgttcatg ggaatttcga tccagcatgg accgggccgg ccggaaacgg ccagagcgta
      841 ggttttctcc agctcgtaga gaattttgag aggatcggca acacgaaccg cgtatttggt
      901 gacctttttg accatgtcca cgctgtcgta gccctgtacg ccccacattc gcaacgggct
      961 ttccggccga gtgaatttgg agttttcgtt tgagacgata atcatcccgg ggatggaatc
     1021 agcccaggcg ctgacgacgc cggtgatgcc gttggtggaa cccgcgcccg tggtgaggac
     1081 cgcggcggtg acggtcccgg aggtgcggta ataggtctga agggccatac aagcggcctg
     1141 ttcgtggtgg acgcagacca tctggatggc ggtggtgcga tagatagaat cgaagatgtg
     1201 tgcgttcccg gaaccaatga tgccgaaggt gtgtttcatt ccttttttga ccagaaaatc
     1261 agcgatgacg tcggaaactt tcactttcat gggaattcgt cctctatttg gtttgatata
     1321 gatctcgatg ccactttgcc gtacggcgga tggcttcgtc cagactgacg cgctcgcgca
     1381 ggcctagttc ggactgcgcc ctctttgtgg acggcacata ttgatcggga gcgatgccgg
     1441 gggacgattg tttggcgaac tccaccggga tgcctgggcc gagcacgtcc gcgattttat
     1501 gggccagttc cttgatggtg acccggttct tggaaccgac attataggga cggcatttct
     1561 cgccgcgaaa gaggattttc cataaccagg ttgcaaggtc cgcgccatag agataagaac
     1621 gcaccggcga gccgtccccc ttgagcctga tcggttcccc cctcagcaca ttgccgatga
     1681 aattgccgat agcgaagtgg cggtcaagcg gttgatatgg gcccacaaag gcaaaacaac
     1741 gggccacctt cgtttgcaga gaagtatctt ttgagaaaat cgagcaaagc gtttctccca
     1801 gccgtttccc ttcgccatag gccgagcggg gagaggccgc atcgggggcg ccccgataag
     1861 tctcggcgat atgcgggacg gattccggtt gatcgccgta cacggcacct gagctgacat
     1921 aaagaaattt ctttacgccg gttttatggg aaaactcaag cactcgtcga gtcccttcga
     1981 cgatgacatc cagcatctgg agcgggtttt cgtcgttgag ttgggcactg gcgtccgtgg
     2041 cggcgtgaat aacgtgagaa aacgcgcctt tcgggaattt gaattttcgc acatcgccac
     2101 gcaaaaagga gaggctcgga ttattggcca ggtgcggcca gcggttccga aaagcgcgag
     2161 gggcccgggt caaaacaacg actttcgctt tcaagcccaa gtgttcattc gcccaaagga
     2221 aactttccag cagccagcag ccaaagaaac ccgttccgcc cgtcaagaaa agccggccgt
     2281 tcttaagatc cctccaatcg tccccgattt cggacaaaac ctgattgagg tcggaagcga
     2341 gcagggtgtt tgaagtcttc ggtgacaagg cgtgagactc aggatttttt aagattgtcg
     2401 aagccgacgg cttccttgac gatgaagttg ggacgttgtt tcacttcttc gtaaatgagg
     2461 ccgatgtatt cactcatgat gccgagcatg gtgaatagaa acccgaacat gaggagcatg
     2521 accgccatga tggtgccgaa gcccgggaaa gggacgcccc aagcgaagac ccggatgacg
     2581 atgaaaacca aaatcaggaa tgagagcgtc gagacgaaaa caccgacggc ggtgatggct
     2641 ttcaggggga cgtaggaata ggacaaaatt cccatgacgg ccagccgcag gacgacgagg
     2701 gtgtgcgatt tggcttcccc ggcgaaacgt ggagcccttt cgtgttccac cccgatggac
     2761 ttgaaaccgg tccagatgaa catgcctcgg aggaaccggt tgcgttccca catgctgttc
     2821 accgcccgat aaactctctt gtccaccagg cgaaaatcgc tggcattgcg ggggaagagg
     2881 ccaccggtca atttgttgac gagaatgtag aaaagctggg aattgaaccg gcgaaggagg
     2941 ctggaaccgt ggcgtttggt gatgacgccg tagacgttct cgtagccttc ttcccatttt
     3001 tggatgaacg tggtgatgag ttccggcggg tcctgaagat cggcggtcat gaccacggcg
     3061 gcgtcgccgg aggcgtgttg aagaccggct gtgattcccc cgtcgcattt gaagttgcgg
     3121 gaaagaagga cgattttaaa gcgtttgtcc ttttcgtgca gttccttcaa tttttcgaaa
     3181 gtgctgtcgt gggagccgtt ttcaacgatg atcgcctcga aatcgtattt ggggttcttc
     3241 tggaaaacgc cttggagccg ccggccgagc tcttcgatat tcccctcctc gttgtaggcg
     3301 gggatgacga cagagatcag ttttttcgtg gccggctcag acattcgatt acctcggagt
     3361 ctttggggtt aagcggattt tttgaaaaaa gattcgaact gttcaagcat gtaggtgatc
     3421 atgccgtcgg tgatgcccgg ataaacgccg atccagaacg tgccgttcat cacctgatcg
     3481 gtgtttttaa ggtcgcccac ggtgcgatat tcgatccctt tgaagtaggg ctgacggaca
     3541 atgttaccgg caaagaggag gcgagtgccg atcttgttgt tttcgaggtg acgggtaagg
     3601 tcttcgcggc gaaagggggc ggtgctcttc accgtgatag gaaatccgaa ccaggcgggt
     3661 tccgagccgg gggtcggttc ggggaggatt aggaactcgg aatatttcga cagccctttg
     3721 tgaaggaggt cgaaattgtg tcgccgccgc cggatgaagt cgtcaagttt gttcagctgg
     3781 gacacgccca ccgcggcctg catatccgtg gctttgaggt tgtagccgac gtgggaatac
     3841 acatacttgt ggtcgtagcc gtcgggcaga tcgcccagtt gccaatcgaa ccgcttgccg
     3901 caggtgttgt cttttccggg ggcgcaccag caatcacggc cccagtcacg gacggattcc
     3961 aagatggaat ggaggcggtt gttggcggtg gccaccgcgc cgccctctcc catggtgatg
     4021 tgatgtgcgg gataaaacga gaacgtggcg agttggccga aagtccccac gggttttcct
     4081 ttgtacaggg agccgagtgc gtcgcaggaa tcctcgatga gccaaaggtt atgcttttgc
     4141 gccagatgga cgacggcgtc gagctcgaag ggattgccga gtgtgtgggc gatcatgatg
     4201 gcgcgcgttt tgggggatac ggcttcggcc aaacgttccg ggcgcgggcc gtaggtgccc
     4261 aactccacat caatgaaaac ggggacaagg cggttttgga tgagggggtt caccgttgta
     4321 gggaaagaag ccgccagggt gacgacttcg tcaccgggct tcaactgtct gtctttgagc
     4381 gtcggggagg tcaaggcaga aaccgccaga agattggcgg aggagccgga attcaccagt
     4441 acggagtaac tcagtcccag tcgcttggcc agttttttct cgaactcgtc cccgaaccgg
     4501 ccggtggtga gccagaaatc cagcgaagcg tcaatcagat ggagcatgtc gtcggcatcg
     4561 tagactttgc cggaaactgg gacggggctt tccccgggaa cgaatttttt ggggccgtgg
     4621 gagcgtttga agaattcccg tacctccctg taaacggcct cccgattttc ggcggaagct
     4681 gcgg
//
LOCUS       DAOTFD010000207         4635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_13493_length_4635_cov_3.846070, whole genome shotgun sequence.
ACCESSION   DAOTFD010000207 DAOTFD010000000
VERSION     DAOTFD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13493_length_4635_cov_3.846070"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="P5079_09810"
     CDS             complement(<1..547)
                     /locus_tag="P5079_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30315.1"
                     /translation="MTRARRALFALVLLSSTALPAHAGLEEARARLLQGDFSGAVLSY
                     SGIRTTHDPERAAEYAYALALAGFDDLALSRLDQAGILLNKNRPEKNGTVNFFAAQVF
                     ALLGDDDIAGELALQSAPRPAWMAGAVPPPRSSPAPRRPSAFSADMAVANNLLVQKRY
                     YTAARRFQRMGGSYPREPEVHA"
     gene            complement(544..2409)
                     /locus_tag="P5079_09815"
     CDS             complement(544..2409)
                     /locus_tag="P5079_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30316.1"
                     /translation="MKRSVCVVFLTLGCLPASLLAGWTFSYQLTVVGVCGGVSAPPLP
                     PIPAAIPTQQMCETLRATVDAISETGCTDEGCCTAGYTVGPCVYVADSGGDLGGTGTG
                     GGTQLTVTLPNAALFPDGSLLPQNQFTQIAGGFYNLGNVSLLGTDYGDASFSSHYLYS
                     TEEWFRQFQERFRLFYEQNESLLRQAGLSIYGAAGEAGSPEQSIFENWFARMTFQPHG
                     LPEDARITALLERMRELEELYDRSYDPVLADQLEDLARRVEDIRRDLFRKQLEKGGLS
                     PQQIDATLKNLENFSNNFGRNPVIAEISQADIADAKHRPGAARSWDDYLNRYYKDNVG
                     DDPYVFPREGMSREDQQKFVQDINRKAREIYESHGLEKSFDALSEKEKRDWAIVARSR
                     QLFDGCPADKQRDMEKVWTLQERYNATLKRPGAEQDALAAAAKDGKGSDIAYSSAKGG
                     VQSHYDCVLYAMANAADEPYSVTSIVALEKIRNDPWRDQKERDNPALALEDPKKGGGG
                     GMTDREILQVGQALGPTEIVRREDIARALEETGKPVFAAIDIGGGSHAIAITKVFEKD
                     GQTYYEVMDSNSRTKDSKTYWKKDDLEKSIVTGGLIVLPEKGTVPQLLREPEKKK"
     gene            complement(2531..2602)
                     /locus_tag="P5079_09820"
     tRNA            complement(2531..2602)
                     /locus_tag="P5079_09820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2568..2570),aa:Gln,seq:ttg)
     gene            complement(3011..3325)
                     /locus_tag="P5079_09825"
     CDS             complement(3011..3325)
                     /locus_tag="P5079_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HRY30317.1"
                     /translation="MEITEIRVTLRNEEKLKAFVTVTFDKCFAVRNMKIVEGEKGPML
                     CMPSRKLPDGTFKDVAHPINAEFRSYLEGKIFAAYQEECRKGGATPPTAASPASHPPA
                     SV"
     gene            complement(3355..4278)
                     /gene="ispE"
                     /locus_tag="P5079_09830"
     CDS             complement(3355..4278)
                     /gene="ispE"
                     /locus_tag="P5079_09830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRY30318.1"
                     /translation="MTKGSVRRVRLEACAKVNLFLEIVRRRPDGYHELKTLFQEISLG
                     DTLEVAASPSATPVDPEKGIETFRCVGAALPTDNGNLVSRAIRAFRGDGPWDRRYRLV
                     LRKKIPVGAGLGGGSSDAAAALRACWHLDGNGRRAFPWERYVPLAARLGADVPFFLRG
                     GLAEASGIGDRLTLLRPPSRRLWFVLIFPRVFVSTKWVYQRLRFPLTKHRSWRRLKTH
                     ILRGRPAREWAPLLFNRLEEVVVPRVPAVRKAKEALRQAGCLTSLMSGSGSAVFGPVE
                     SEKHGKDVLGRVRSGAWDAWLVSSRCRQESR"
     gene            complement(4287..>4635)
                     /locus_tag="P5079_09835"
     CDS             complement(4287..>4635)
                     /locus_tag="P5079_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30319.1"
                     /translation="VLGGFLSGDLIARFDDPAVEVEQKGNELVYTASFGELRIDGKTK
                     TISRLLWRDASVSAIPVEFRLSGYQDVEGLLLPFRMDCEISRGTNFSLDFKRLKINFP
                     VDAAQFQLPPAAR"
BASE COUNT          871 a         1498 c         1413 g          853 t
ORIGIN      
        1 ccgcgtggac ttccggttcc cggggatacg aaccccccat ccgttgaaac cggcgggcgg
       61 cggtgtaata gcgcttttga acgagaaggt tgttggccac cgccatgtcc gccgaaaaag
      121 ccgacggccg ccggggagcc ggcgaagagc ggggaggcgg aacggcgccg gccatccagg
      181 ccggccgggg cgcggattga agggccagtt ccccggcgat gtcgtcgtcc cccagaagcg
      241 caaacacctg cgccgcgaaa aaattcacgg tgccgttctt ctccggcctg tttttgttca
      301 aaaggatgcc ggcctgatcc agccggctga gcgccaaatc atcgaagccc gccagagcca
      361 gcgcgtaggc gtattcggcc gcccgttcgg gatcgtgggt ggtgcgaatg ccggaatagg
      421 acagcaccgc gccggagaag tccccctgga ggagccgtgc ccgggcttct tccagccccg
      481 cgtgcgccgg caaggccgtg gacgagagaa ggacgagcgc aaaaagagcg cggcgggcgc
      541 gggtcatttc tttttctccg gctcgcgcag caactgaggc accgtgccct tttccggcag
      601 gacaatgagc ccgccggtga cgatcgattt ctccagatcg tcttttttcc agtacgtctt
      661 ggaatccttg gtgcggctgt tggagtccat gacctcgtaa taggtctgcc cgtccttctc
      721 aaaaaccttg gtaatcgcga tggcgtggct cccgccgccg atgtcgatgg cggcgaacac
      781 cggtttgccg gtctcctcca gagcccgggc gatgtcttcc cggcgaacga tctccgtcgg
      841 ccccagggcc tgtcccacct gcaggatctc gcggtcggtc atccccccgc cgccgccttt
      901 cttgggatcc tccagggcca gggcggggtt gtcgcgttcc ttctggtcgc gccagggatc
      961 gttccgtatc ttttccaggg cgacgatcga ggtgaccgaa tacggctcgt ccgcggcgtt
     1021 ggccatggcg tagaggacgc agtcgtagtg cgattggacc cctcccttgg cggaggagta
     1081 ggcgatgtcc gagccttttc cgtccttggc ggcggccgcc agggcgtcct gttcggcgcc
     1141 gggccgtttc aacgtggcgt tgtatctttc ctgaagcgtc caaaccttct ccatgtcccg
     1201 ctgtttgtcg gcgggacagc cgtcgaagag ttgccgggac cgggcgacga tggcccaatc
     1261 gcgcttctct ttctccgaga gggcgtcgaa cgatttttcg agcccgtggg actcgtagat
     1321 ctcccgggct ttcctgttta tgtcctggac gaatttttgc tggtcctccc gggacatccc
     1381 ttcccgcgga aaaacgtagg ggtcgtctcc cacgttgtcc ttgtagtatc tgttcaaata
     1441 atcgtcccag gagcgggcgg cgccgggccg atgtttggcg tcggcgatgt ccgcctggga
     1501 gatctccgcg atcacggggt tccggccgaa attgttggaa aagttttcca gattcttcag
     1561 ggtggcgtcg atctgctggg gagacagccc ccctttctcc agctgtttcc ggaaaaggtc
     1621 ccgccggata tcctcgacgc gccgggccaa gtcctcgagc tgatcggcca gaaccggatc
     1681 gtaggaccgg tcgtacagct cctccaattc ccgcatcctc tccaggaggg ccgtgatgcg
     1741 ggcgtcctcg gggaggccgt gcggctggaa cgtcatgcgg gcgaaccagt tttcgaagat
     1801 ggattgttcg ggagaaccgg cctccccggc ggcgccgtag atggagagac cggcttggcg
     1861 gaggagggat tcgttttgtt cgtagaaaag acggaaacgt tcctggaact gtcgaaacca
     1921 ttcttccgtg gaatagaggt aatgggagga gaaagaggcg tcgccgtaat cggtgcccag
     1981 cagggaaacg ttccccaggt tgtaaaaccc gccggcgatc tgcgtgaact ggttttgagg
     2041 caggaggctt ccgtccggga acaacgccgc gttgggcagg gtcacggtca gttgcgttcc
     2101 gccgcccgtc cccgtcccgc cgagatcccc gccggaatcc gccacgtaca cgcagggccc
     2161 cacggtgtat cccgcggtgc agcatccttc gtcggtgcat cccgtttcgc tgatggcgtc
     2221 gacggtggcg cgaagcgtct cacacatttg ctgggtggga atcgccgccg gaatgggcgg
     2281 caacggcggg gcggaaacac cgccacagac gcccacgacg gtgagttggt aggagaacgt
     2341 ccagccggcc agaagggacg cggggagaca tcccagtgtc aaaaaaacga cacacacgct
     2401 tcgcttcacg acacggctca tacgaaaaga attataacct cgatttttaa aaattaccaa
     2461 cgaaaccgtg cctcggttca acaccccgat cttcggattt cagggacccc gaaacggggt
     2521 ccctgaaatc ctggggcgct aggatttgaa cctaggaatg acggctccaa aggccgttgc
     2581 cttaccactt ggctacgccc caaaaatcat cgcttcaatt ccgaaatcgc caaagcaccg
     2641 cccgacgttt cggcaactcc gccctccggc gcacatcggc ctcgtcggaa gcgccgccgg
     2701 gccggggcgc tcacgaactc gcgcccgcgc tcaacggccc acgttcccaa aggtccaccg
     2761 gacctttgac ctgggccagc gcccctacca tttggctacg ccccaaaaaa tcagtgtaac
     2821 aagggctgtc ctcgcgcaaa agacagacgc tttccggccg tcgacgtgtt gcgcaagggc
     2881 gcccccaagg gacttcgacc cttttcgacg aaccgccgcc cggccttgaa gaatcccgcc
     2941 catggccggg caataaaaaa tcacttgccg gggttttccc ggacgagtga gtgtttttca
     3001 gccccaacgc tcagacggag gccggggggt ggctcgcggg agacgccgcg gtcggcggcg
     3061 tcgcaccgcc cttgcggcat tcctcttgat acgccgcgaa aatcttccct tccagatacg
     3121 accggaactc ggcgttgatg gggtgcgcca cgtccttgaa ggtgccgtca gggagtttgc
     3181 gcgagggcat gcagagcatg ggccccttct ccccttcgac gatcttcata ttacggacgg
     3241 caaagcattt gtcgaacgtg accgtgacaa acgccttcag tttctcttcg tttcggagag
     3301 tgacgcgaat ctctgtgatt tccacgaggc acctctctgc gtttcacgct gtcgttaacg
     3361 actctcttgt ctgcaacgag aggataccaa ccacgcatcc cacgccccgg accgcacccg
     3421 gcccagaacg tctttgccgt gtttctccga ttcgaccggt ccaaaaaccg ccgagccgga
     3481 acccgacatc agcgatgtca aacagccggc ctgccgcaac gcctccttgg ctttgcgaac
     3541 cgcaggcacc cgcgggacca ccacttcttc gagccggttg aacagcaacg gcgcccattc
     3601 tcgcgccggc cggccccgta aaatgtgtgt cttaagtcta cgccatgaac ggtgttttgt
     3661 caacggaaag cgcagacgct gatagaccca tttcgtggac acgaaaacac gcggaaaaat
     3721 caacacgaac caaagacgcc gggacggggg acgaaggagg gtcagacggt cgccgatccc
     3781 cgaggcctcg gccagtccgc cccgcagaaa gaacgggacg tccgccccca gccgggccgc
     3841 cagcggaacg taacgttccc agggaaaagc acgccggccg tttccgtcca aatgccagca
     3901 cgccctcagg gcggcggcgg cgtcgctgga accgccgccc agacccgccc ccaccggtat
     3961 tttttttcgc agaacgagcc ggtatcgccg gtcccacggc ccgtcccccc gaaacgcgcg
     4021 gatggcccgc gacaccaggt tgccgttgtc cgtgggcagg gccgccccga cacaacggaa
     4081 ggtttcgatc cccttttccg gatccaccgg cgtcgccgac ggggacgcgg ccacctccag
     4141 cgtgtccccc agcgatatct cctggaaaag cgtcttcagc tcatgatagc cgtccggccg
     4201 gcggcggacg atctccagga acaaattgac cttggcgcag gcctcaagac ggacgcggcg
     4261 aacggaccct ttcgtcatgg cgaaattcag cgggcggcgg gagggagttg gaactgggcg
     4321 gcgtccaccg gaaaattgat cttcagccgc ttgaagtcca gcgagaagtt cgttccccgg
     4381 gagatctcgc agtccatgcg gaacggaagg agaagcccct ccacgtcctg atagccggac
     4441 aggcggaact ccaccgggat cgccgacacg gaggcgtccc tccaaagcag ccgggagatc
     4501 gtctttgtct ttccgtcgat ccgcagctcg ccgaaggacg cggtgtaaac gagctcgttc
     4561 cccttctgct ccacctccac cgccggatcg tcgaaccggg cgatgaggtc ccccgagagg
     4621 aacccgccca ggacc
//
LOCUS       DAOTFD010000208         4496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_14078_length_4496_cov_2.690160, whole genome shotgun sequence.
ACCESSION   DAOTFD010000208 DAOTFD010000000
VERSION     DAOTFD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14078_length_4496_cov_2.690160"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4496
                     /locus_tag="P5079_09840"
     CDS             <1..>4496
                     /locus_tag="P5079_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30320.1"
                     /translation="ITTRSGMNAKGDYTEVRRTLGRWGNAELDSTDETVRFNSVFDGF
                     GRMMSYSEATTQDGLAGTLTTRELSGLLYDVDGNMVGSHEVSVNAQGAVSTMDRAMKY
                     DPQGRLSEYTADSKNTGDGISLGSHVTRTGIAYNGVGDVVSYKEKTTKDENFIEENVD
                     WSGQYNVLGQLANSHQVTHKTGVDTDGSVMDITDVTDTYAMTYNRQGNMAGYTEELTS
                     SPTPALVTITVMGGMTYDDAGRTSGFTQTVHQVSRGAVSHDPTGYFRINDNAALHSQS
                     TTVRAQAQYNSLGMLVSYADTVTENGLQTQSTVKNAYDSSGRLWSSTTESQKTGEEKR
                     LYYYADGQEMNEWQLSALLALHPGKTVEDLVADGALQAVRRTEKVDEKWTTVRSGLSF
                     DGLNRLRGSTDTTTNSDGTVSVNTSSDMVYDREGRLIGSRSDSTQTGTVGQLVYRLNG
                     VEVTAEDINSVLAATGLTLWDLFNGTDPQNKLEAVEVNRSLDKSSYNVRAGITYNDLG
                     QMIRYQDSTSDPAGEIDSQVTTVQMSYTRHGQVQTQTSLSDTRYVNGGKNESTTLLTF
                     NYDPATGQMLGAGSVGTFTSTDAPVWSDTNQDGQVDSLVTLSPTYGNTQQSYGVFNGQ
                     MRLLNQTSSSSTVSPDGTTSTSHSTTIYIYNALGRLVGATSEGVSTTTDVWETATTST
                     SSQLMTAIQGEMKAILSVSDQFTENLDTSSNRTLTATTYTYDDTGKGLYGIGVGKSFA
                     DDGFGTVTVSTIGQLFAYVGGQFRLVKTDTISDTYDDLQTGTGEPGQEPEGSSAEPPP
                     ELGVFGNNWMQALAGLPFIITWDLHEQIYNALSAVLNFTFDTFSVQQTNVNVSDVIGD
                     AVREALAQLGQSGSVLALKDVKLVRGTPTDPNIMDTDDMVTWTSDGVMIIHEDVVEQW
                     LVAGRDIVQDLMLTILHENHEYVRYAEAKQSGLEVNWELMRTFHEESVALGYQPQGIE
                     ENWLPTGGVASGSAGGTFDMGGALNALNALDPTQTANIAQQIAGQTGLPLNNLAVDYG
                     NGSGVTEAALVGLDSYYNSVDKTFNPAAHGYTVRTPPGDVGDFTIYEKNGGKDVFIVY
                     REDLNAARDVNNDGTIGEHEGQTDINGDGTIDGNDVQWSATTFIHMVKGADDKMIYRT
                     TYNLGSRENPDTSKTVEWRNPANPSEFATLTDSIHYDDHDDDPDTIPNPSDPGVHVNK
                     SRVLMAKTGDGPNRIVGKQSLYDGMRLQSAVDGQPNVYAHIKTASDMYRYGDTKVTKN
                     SYTVNLSNNNIATFATLVKNLPDGPLFGVVYGEVNQSNDPDAPNYVANAMRIINGVGD
                     SGRFDFTKNASGVWVPSPSNPQNPSFTINAANIPNVGYQVTVTWGGFSFSVTDNNGSI
                     YVGGNVPPGALNSSSQGLEGLLVGEGNAAQPLGGGMPEQFTVPVGGDGATGGGAASSS
                     LFGTVDWTKVLNAVGALSNKGRDLLRTVVAAVNPAQLRLPRFSLPFGKMGGEGALADI
                     SALV"
BASE COUNT          960 a         1428 c         1367 g          741 t
ORIGIN      
        1 atcaccaccc gttccggcat gaacgccaaa ggcgattaca ccgaggtccg gcgcaccctc
       61 ggccggtggg gcaacgcgga gctggattcc acggacgaaa cggtccggtt taacagcgtt
      121 ttcgacggtt tcggccgcat gatgagctac agcgaggcca ccacccagga cgggttggcg
      181 ggcacgctca ccacccggga gctttccggc ctgctgtacg acgtggacgg caacatggtg
      241 ggcagccatg aggtgagcgt caacgcccag ggcgcggtct ccactatgga ccgggcgatg
      301 aaatacgacc cgcagggacg gttgtcggaa tacaccgcgg attccaaaaa caccggcgac
      361 ggcatctcct tgggcagcca tgtgacgcgt accggcatcg cgtacaacgg cgtcggcgac
      421 gtggtttcct ataaagagaa gaccaccaag gatgagaatt tcattgagga gaacgtcgac
      481 tggtccggcc aatacaacgt gctggggcag ctggccaatt cacatcaagt gacgcataag
      541 acgggggtgg acacggacgg cagcgtcatg gacatcaccg acgtgaccga cacctacgcc
      601 atgacgtaca accggcaggg gaacatggcg ggttacaccg aggaactgac gagttcgccc
      661 accccggccc tggtcaccat cacggtgatg ggcggcatga cctacgacga cgccggccgg
      721 acctccggtt tcacccagac ggtccaccag gtctctcgcg gagcggtttc tcacgatccc
      781 accgggtatt tccggatcaa tgacaacgcc gccctccatt cccagagcac cacggtccgc
      841 gcccaggccc aatacaattc cttggggatg ctggtgagct acgcggacac cgtgacggag
      901 aacggtctgc agacgcagag cacggtgaaa aacgcctacg attcctccgg ccggctgtgg
      961 agttccacca cggaaagcca gaagaccggc gaggaaaaac ggctttacta ttatgccgac
     1021 ggccaggaaa tgaacgaatg gcagctctcc gcccttttgg ccctccaccc cggcaagacg
     1081 gtggaagatt tggtcgccga cggggccctc caggcggtcc gccggaccga aaaagtggat
     1141 gagaaatgga ccacggtgag gagcgggctg tctttcgacg gtttaaaccg ccttcgcggt
     1201 tccacggaca ccaccaccaa ttccgacggc accgtgtcgg tgaacacctc gtccgacatg
     1261 gtctacgacc gggaaggccg cctcatcggc tcccgcagcg attcgaccca gaccggcacg
     1321 gtggggcagc tggtgtatcg gttgaacggc gtggaggtca cggccgaaga catcaattcg
     1381 gtgttggccg ccaccgggct gaccctgtgg gaccttttca acgggaccga cccgcagaat
     1441 aagctggaag cggtggaggt gaaccgttcg ctggacaagt cgagttacaa cgttcgcgcc
     1501 ggcatcacct acaacgattt gggacagatg atccgttacc aggactccac cagcgatccg
     1561 gcgggggaga tcgacagcca ggtgaccact gtgcagatga gctacacccg gcacgggcag
     1621 gtgcagacgc agacctccct cagcgacacc cgttatgtca acggcggcaa gaacgaatcc
     1681 accactctcc ttacctttaa ttacgatccc gccaccggcc agatgctggg cgcgggttcc
     1741 gtggggacgt tcacctccac cgacgccccg gtgtggagcg acaccaatca ggacggtcag
     1801 gtggattcgc tggtgacctt gagtccgacc tatgggaaca cccagcagag ctacggggtg
     1861 ttcaacgggc agatgcggct tctgaatcag acctcttcca gttccaccgt ttctccggac
     1921 ggcaccacca gcacctccca ctcgacgacg atctatatct acaacgccct cggccggttg
     1981 gtgggcgcca cctccgaagg ggtgagcacc accaccgacg tgtgggagac ggccaccacc
     2041 tccacatcgt cccagctgat gaccgccatc caaggggaga tgaaggcgat tctgagcgtg
     2101 tccgaccagt tcaccgaaaa cctggacacc tcctccaacc gcactctcac cgccaccacg
     2161 tatacctacg acgacaccgg caaggggctt tacggaatcg gcgtgggcaa gtcctttgcg
     2221 gacgacgggt tcggcaccgt gacggtgtcc accatcggcc agctgttcgc ctatgtgggg
     2281 gggcagttcc ggctcgtcaa gaccgacacc atttccgaca cctacgacga cttgcagacc
     2341 ggcaccgggg agccgggcca ggaaccggag gggtcctccg ccgaaccgcc tcccgaactc
     2401 ggtgttttcg gcaacaactg gatgcaggcc ttggcggggc ttcccttcat catcacctgg
     2461 gacctgcacg agcagatcta caacgccctg agcgccgtcc tgaatttcac gttcgatacg
     2521 ttctccgtcc aacagacgaa cgtgaacgtc tcggacgtca tcggggacgc cgtccgggaa
     2581 gccctggcgc aactcggcca gtcgggatcg gttttggcgt tgaaggacgt gaagctggtg
     2641 cgcggcaccc ccaccgatcc caacatcatg gacaccgacg acatggtcac ctggacctcc
     2701 gacggcgtga tgatcatcca cgaggacgtg gtggaacagt ggctggtggc cggacgggac
     2761 atcgtccagg acttgatgct caccattttg cacgagaacc atgagtacgt gcgctatgcg
     2821 gaggccaaac agtcggggtt ggaagtcaac tgggagctga tgcggacgtt ccacgaagag
     2881 tcggtggcgc tcggatatca gccgcagggc atcgaggaaa actggctgcc caccggcggg
     2941 gtggcgtccg gttcggcggg gggaaccttc gacatgggag gcgccctgaa cgccttgaac
     3001 gcgctggatc ccacgcagac cgccaacatc gcccagcaga tcgcgggcca gacggggctc
     3061 ccgctcaaca acctcgccgt cgactacggc aacgggtccg gcgtgacgga ggcggcgttg
     3121 gtcgggctgg actcctacta caacagcgtg gataagactt tcaatcccgc cgctcacggg
     3181 tacacggtcc ggacgccgcc cggcgacgtg ggcgatttca ccatctatga gaagaacggc
     3241 ggcaaggatg ttttcatcgt ctatcgggag gacctgaacg cggcccggga cgtgaacaac
     3301 gacggcacca tcggcgagca cgaaggacag acggacatta acggggacgg caccatcgac
     3361 ggcaacgacg tccagtggtc ggcgacgacc ttcatccata tggtgaaggg ggcggacgac
     3421 aagatgatct accgcaccac ctacaacctg ggaagccgcg agaaccccga cacctccaag
     3481 accgtggagt ggcgcaaccc ggccaacccc agcgagttcg ccacgctgac ggattcgatc
     3541 cattacgacg atcacgacga cgatccggac accatcccca atcccagcga tcccggcgtg
     3601 cacgtgaata aatcgcgggt gttgatggcc aagacgggcg acggccccaa ccgtattgtg
     3661 ggcaagcaaa gtctttacga cggcatgcgg ctgcaatcgg ccgtcgacgg gcagcccaac
     3721 gtctacgccc acatcaagac cgccagcgac atgtatcgtt acggcgacac caaagtcacg
     3781 aagaactcct acaccgtgaa cctctcgaac aacaacatcg ccaccttcgc caccctggtc
     3841 aagaatctcc cggacggtcc tctcttcggc gtggtctacg gcgaggtcaa tcaatccaac
     3901 gatcccgacg cccccaacta cgtggccaac gccatgcgga tcatcaatgg agtgggggat
     3961 tccggccggt tcgacttcac caaaaacgcc tcaggggttt gggtcccttc gcccagcaat
     4021 ccccaaaatc ccagcttcac catcaacgcc gccaatatcc cgaacgtggg gtatcaggtc
     4081 acggtcacgt gggggggatt ctctttttca gtgacggaca acaacggttc catctatgtg
     4141 ggaggcaacg ttccgcccgg ggcgttgaac tccagttcgc aggggttgga gggtcttttg
     4201 gtcggcgaag gcaacgcggc acagcctctc ggcggcggaa tgcccgaaca attcaccgtg
     4261 ccggtcggcg gggatggcgc aaccggcggc ggggcggcct cttcttcgct cttcggcacc
     4321 gtggattgga ccaaggtgtt gaacgcggtg ggggctcttt ccaacaaggg acgcgacctg
     4381 ctccgcaccg tggtcgccgc ggtcaatccg gcccagctcc gattgccgcg gttcagtctc
     4441 cccttcggca agatgggggg cgagggggcg ttggccgaca tctccgcgtt ggtgaa
//
LOCUS       DAOTFD010000209         4424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_14424_length_4424_cov_4.349508, whole genome shotgun sequence.
ACCESSION   DAOTFD010000209 DAOTFD010000000
VERSION     DAOTFD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14424_length_4424_cov_4.349508"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="P5079_09845"
     CDS             <1..1334
                     /locus_tag="P5079_09845"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reversible formation of D-erythrose
                     4-phosphate and D-fructose 6-phosphate from sedoheptulose
                     7-phosphate and D-glyceraldehyde 3-phosphate; catalyzes
                     isomerization of glucose 6-phosphate and fructose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRY30321.1"
                     /translation="LFVVSSKSGGTIEMQSLFKYFHARLETLKGENAGENFIAITDPE
                     TSLEKLAHQKHFRTLFITPPDVGGRYSALTYFGLVPAALMGVSAPELLERASQMARAC
                     AATLPAAENTGLKLGAALGVLAKAGRDKVTFVMSPKFASFGTWVEQLIAESTGKEGKG
                     LIPVDGEELASPAAYGDDRVFVSLSMERSRDAGLEKKLAALEKAGHPVVRIPLTDAYD
                     LGREFLRWEIASAVAGILLGINPFDEPNVTESKQNTGRLLEAFKSAGKLAEDPPLWER
                     DGIRLWADPSSAAAVGRSGPAAAGTVGELLAAHVRQAVAGDYVSVMAYLTPDGAHHAL
                     LQRLRHRLFALTRAATTLGYGPRFLHSTGQLHKGGPNKGVFVQIVARDAENPAVPGES
                     YGFSVLKNAQASGDFEALKNHACRVLRLQLSSSTVKDLQGLVRSLEKVKAG"
     gene            1367..2263
                     /gene="gnd"
                     /locus_tag="P5079_09850"
     CDS             1367..2263
                     /gene="gnd"
                     /locus_tag="P5079_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRY30322.1"
                     /translation="MELGFIGLGRMGANMVERLLRGKHRVVAYNRTEQKTRDIMKKGA
                     AGAFSLEELVAQLKPPRAVWVMLPAGDALQKMLDALTPLLAPGDLIVDGGNSRYSDSV
                     RRADDLRAKKIHFMDAGTSGGIWGLEIGYCLMVGGEKEDFRRLEPALATLAPPDGYLH
                     CGPAGSGHFVKMVHNGIEYGMMQAYAEGFEIMKSAPFKLEMRDVARLWNRGSVVRSWL
                     LELLDRALSKDPELASLEGYVEDSGEGRWTLQQAVDAAVPAPVLAASLFARFASRRPE
                     SFAAKVLAALRNEFGGHAVKKK"
     gene            2394..3923
                     /gene="zwf"
                     /locus_tag="P5079_09855"
     CDS             2394..3923
                     /gene="zwf"
                     /locus_tag="P5079_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRY30323.1"
                     /translation="MGPLRLSAVQSSVQQQFCIETRPDPCGLVIFGASGDLAERKLFP
                     ALYRLRRNGFLPEKFFAVGMGRTPMTDDAFRDRVRAALGPASPNDAPLKTFLSSFYYQ
                     TGDYADPAAYKKLSDRLDELARSHHTRGNVLFYFSVPPTVYLPIVRCLGGVGLNRPKD
                     AGSWRRIVVEKPFGRDLKSAEDLSEEIEKVFSDDQIYRIDHYLGKETVQNILVLRFAN
                     LLFEPLWNRRYIDHVQITVAETLGVGHRAGYFEQAGLLRDMFQNHLLQLLCVTAMEAP
                     ASFHACRVREEKVKVLRSLRPFRPDTLDRSAVHGQYGPGAIDGQPVKGYRQEPGVAPD
                     SRRETFAAMKVYVDNWRWQGVPFYLRSGKRLPRRVSEIAIQFKHVPHLLFEPLRPQDL
                     SPNVLLLRIQPEEGISVSFETKHPGPKLCMSLVTMDFNYESAFGEPPEAYERLFLDCM
                     AGDQTLFTSKDWVRLSWKFLTPLLESWAETPPLPYEAGSWGPKAANALPAAEGYFWRN
                     L"
     gene            3920..>4424
                     /gene="pgl"
                     /locus_tag="P5079_09860"
     CDS             3920..>4424
                     /gene="pgl"
                     /locus_tag="P5079_09860"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRY30324.1"
                     /translation="MSASRAPVVFRDGEELADALARELLFIARRAVSARGRFVLALAG
                     GTTPAVLYRRLARPPYKKSFPWRFTHFFWGDERFVPPTHPESNYRRVREALLDRVRVP
                     PENRHPVPTGLGTPAAAAKAYERELRRFFSSRPFPRFDVVLLGVGEDGHVASLFPRSA
                     ALNERKRW"
BASE COUNT          732 a         1412 c         1437 g          843 t
ORIGIN      
        1 ccctgttcgt cgtctccagc aagtcgggcg gaaccatcga gatgcagtcc ctgttcaagt
       61 atttccatgc ccggctggaa accctgaaag gcgaaaatgc cggggagaat ttcatcgcca
      121 tcacggaccc cgaaacctcc ctggaaaaac tggcgcacca gaaacatttc cgcaccctct
      181 tcatcacccc gccggacgtg gggggccgct attccgcgct cacctatttc gggctggtgc
      241 cggcggcgct gatgggcgtg tccgctccgg aactcctgga gcgggcctct cagatggccc
      301 gggcctgcgc cgccaccctg ccggcggcgg agaacaccgg gttgaaactc ggcgcggcgc
      361 tgggcgtcct ggccaaggcc ggccgcgaca aggtcacctt cgtgatgtcg ccgaagttcg
      421 cttctttcgg cacgtgggtg gaacagctga tcgccgaaag caccggcaag gaagggaagg
      481 gcctcatccc cgtcgacggc gaagaattgg cttccccggc cgcctacggc gacgaccgtg
      541 tgttcgtctc cctgtcgatg gaacgttccc gggatgcggg cctggagaaa aagctggcgg
      601 cgctggagaa ggccggccat ccggtggtgc gcatcccgtt gacggacgcc tacgacctcg
      661 gccgggaatt cctgcggtgg gagatcgcct cggccgtggc gggcatcctg ctcggcatca
      721 atccgttcga tgagcccaac gtgacggaga gcaagcagaa caccggccgc ctgttggaag
      781 ctttcaagtc cgccggaaaa ttggccgagg atccgcctct gtgggaacgg gacgggatcc
      841 gcctgtgggc cgacccctcg tcggccgcgg ccgtgggccg gtccggtccg gccgccgcgg
      901 ggaccgtcgg cgagctgctc gccgcgcacg tccgccaggc ggtggccggc gattacgtat
      961 cggtgatggc ctatctcacg cccgacggcg cgcatcacgc gttgctgcag aggttgcggc
     1021 accgcctctt cgcgctgacc cgcgccgcca ccacgctggg atacggcccc cggttcctcc
     1081 actccaccgg ccagctccac aagggcggcc ccaacaaggg cgttttcgtc cagatcgtgg
     1141 cgcgggacgc ggagaacccg gccgtgcccg gcgagtccta cggtttctcc gttctgaaaa
     1201 acgcccaggc ctcgggagat ttcgaggcgt tgaagaacca tgcctgccgg gtgctgcggc
     1261 ttcagctgtc ttcatcgacg gtgaaagacc tgcaggggct ggtccgttct ttggagaagg
     1321 tgaaggccgg ctgagctgag gtcacggccg gcggaaagga gtctttatgg aactcgggtt
     1381 catcggattg gggcgcatgg gcgccaatat ggtggaacgc ctcctgcgcg gcaaacaccg
     1441 cgtggtggcc tacaaccgca ccgaacagaa aacgcgggac atcatgaaga agggcgcggc
     1501 gggcgcgttc tctctggaag aactggtggc gcaattgaag ccgcctcgcg cggtgtgggt
     1561 gatgctgccg gcgggcgatg cgctgcagaa gatgttggac gcgctgaccc cgctgttggc
     1621 gccgggcgat ctgatcgtcg acggcgggaa ctcccgttat tccgactccg tccgccgggc
     1681 cgacgacctt cgcgcaaaga aaatacattt catggacgcc gggacctcgg gcggaatctg
     1741 ggggttggag atcggctatt gcctcatggt gggcggggaa aaggaagatt ttcgccgcct
     1801 ggaaccggcg ttggccacgc tggccccgcc cgacggctat ctccattgcg ggccggcggg
     1861 atccggccat ttcgtcaaaa tggtccataa cgggatcgaa tacggcatga tgcaggcgta
     1921 cgccgagggg ttcgagatca tgaaatcggc gccgttcaaa ctggagatga gggacgtcgc
     1981 ccgcctgtgg aaccggggca gcgtggtgcg ttcctggctg ctggaactgc tggatcgggc
     2041 gctgtccaaa gatccggagt tggcttcgct ggagggatac gtggaggatt ccggcgaagg
     2101 ccgttggacg ctgcagcagg ccgtcgacgc cgccgtcccc gccccggtgc tggcggcctc
     2161 cctgttcgcg cgcttcgctt ctcgtcggcc ggaatcgttc gccgccaaag tgctggccgc
     2221 tctccggaac gagttcggcg gccacgcggt caagaagaaa tagcggggaa ggaacttttg
     2281 ttcggaggcc gcgttttatt catccaaagg gagaggggct tttccggtcc ctttcccgcg
     2341 tccacggcct ccgcgttgac tcggggccgg gttatcaacg aagggagggt ctgatggggc
     2401 ctctccggct ttcggccgtc caatcctcgg tgcagcagca attctgcatt gagacccggc
     2461 ccgacccctg cgggttggtg atcttcggcg cctccggcga cctggccgag aggaaacttt
     2521 tcccggctct ctaccgtctt cgccggaacg gtttcctgcc ggaaaaattc tttgcggtgg
     2581 gcatgggacg cacccccatg accgacgacg cgttccgcga ccgcgtgcgc gccgccctcg
     2641 ggccggcctc ccccaacgac gcgcccctga aaaccttcct ttcctcgttc tattaccaga
     2701 ccggcgacta cgccgacccg gcggcctaca aaaaactctc cgaccgtctg gacgagttgg
     2761 cgcggtccca tcacacccgc gggaatgtcc tcttttattt ctccgtcccc ccgaccgttt
     2821 acctccccat cgtgcgctgc ctcggcgggg tcggcttgaa ccggccgaaa gacgccggtt
     2881 cctggcgccg gatcgtcgtt gaaaaaccgt tcgggaggga tttgaaatcg gcggaggacc
     2941 tttcggagga gatcgagaag gttttttccg acgatcagat ctaccgcatc gaccactacc
     3001 tcggcaagga gacggtgcag aacatcctgg tgttgcgctt tgccaacctt ctctttgagc
     3061 cgctttggaa caggcgctat atcgaccatg tgcagatcac cgtggccgag accttggggg
     3121 tggggcaccg cgccggctat ttcgaacagg cgggcctcct gcgggatatg tttcagaacc
     3181 acctccttca gctcctctgc gtcaccgcca tggaggcccc ggccagtttc catgcctgcc
     3241 gcgtgcggga ggaaaaggtg aaggtgctcc gaagccttcg gcctttccgt ccggacacgc
     3301 tggatcgctc cgctgtgcac ggccagtacg gacccggcgc gatcgacggc cagcccgtga
     3361 agggataccg ccaagaaccc ggcgtggctc cggattcccg ccgggagacg ttcgccgcca
     3421 tgaaggtcta cgtggacaat tggcgctggc agggcgttcc cttttatctc cgttccggaa
     3481 aacggctgcc gcgccgggtt tcggagatcg ccattcagtt caagcatgtg ccgcatctgc
     3541 ttttcgagcc cctgcgcccg caggaccttt cgcccaacgt gctcctcctc cgcatccagc
     3601 cggaagaggg gatctccgtt tcttttgaga cgaaacatcc gggtcccaaa ctctgcatga
     3661 gcctggtgac catggatttc aactacgagt ccgctttcgg ggaacccccg gaggcgtacg
     3721 agcggctttt tctcgattgc atggcgggcg accagacgct gttcaccagc aaggactggg
     3781 tgcggctttc ctggaaattc ctgacccccc tgctggaaag ctgggcggaa accccgccgt
     3841 tgccctacga ggcggggtcg tggggaccga aggcggcgaa cgcccttccc gcggccgagg
     3901 ggtatttctg gcgaaatctt tgagcgcttc gcgcgccccc gttgtttttc gagacgggga
     3961 agaactggcc gacgcgctgg cgcgggaact tctcttcatc gcccgtcgcg ccgtgtcggc
     4021 ccgggggcgg ttcgtgctcg cccttgccgg cgggacgaca ccggcggtcc tctatcggcg
     4081 tttggcccgg cctccctata aaaaatcctt cccttggcgg ttcacgcatt tcttctgggg
     4141 cgatgagcgg ttcgtccctc cgacccaccc cgaaagcaac taccgccggg tccgggaggc
     4201 gctcctggac cgggttcgcg ttccgcctga aaacagacat cccgttccga ccggtttggg
     4261 aacgccggcc gcggcggcga aggcgtatga aagggaactt cggcgttttt tttcgtcccg
     4321 gccttttccc cgattcgatg tggttttgtt gggcgtgggg gaggacggcc atgtggcctc
     4381 tcttttccct cgatcggcgg cgctgaacga aagaaaacgg tggg
//
LOCUS       DAOTFD010000210         4384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_14619_length_4384_cov_3.757450, whole genome shotgun sequence.
ACCESSION   DAOTFD010000210 DAOTFD010000000
VERSION     DAOTFD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14619_length_4384_cov_3.757450"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="P5079_09865"
     CDS             complement(<1..1223)
                     /locus_tag="P5079_09865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30325.1"
                     /translation="MKKSVLYLSAAWILLVSGFYLFHQFPPARQFFLMLSLLRPPTLP
                     AAAEAARETFLTTLTAGLFFLSGRSLLRRIGLSTGTPLENALHSIAMGAALFILLFFV
                     LGMLGLWYKVILLGIPMAVFVLAGIDNQELFSPERFARPAAFTSSFRLLDKILVVSTL
                     FVLLFYLIQTLAPETFYDSLVYHLALPQLWLLEHRIVPVPHNLFSGSPMNTELLYALA
                     LALSGDALAKLFHYALGIGCLALLLAVGRRVSSTRAGLAAALLFATPPMMARLFSNSA
                     VEYGTAFFSLAALLALLTGLEKENVLQRRNWLLVCGFFCGVAFGTKYTAWALLPALSL
                     GAFVHWRADPARRPADFGKTLIYLCGPAFLFVLPWLLKNTLFFGNPLYPFLHGLFPSA
                     QGPINWDALQNHAESR"
     gene            complement(1378..2025)
                     /locus_tag="P5079_09870"
     CDS             complement(1378..2025)
                     /locus_tag="P5079_09870"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HRY30326.1"
                     /translation="MNKRLMIAGIILTSLLPACLPPRLVIKEHVEANEVLELAIFPFQ
                     DAGFPGIGDRVADLFSTELLKLPQYNIIEKGLVENRIKNRGLTSQRVDTEQALEIAGE
                     LGADAVLIGEVTSYSPRKYWLFPPAEAAVNARLIDVKTGHIVWSAYHRRNYGLAHWIT
                     TLLPFPPTMALSLLYSPAVENRTEQTVQNIVSSLNREFMRYERRQGVGRKKTGEK"
     gene            complement(2046..3200)
                     /locus_tag="P5079_09875"
     CDS             complement(2046..3200)
                     /locus_tag="P5079_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY30327.1"
                     /translation="MQKRERKLAAIMLTDIVGFSRKMGANEEAALRMLERHNTLLSAI
                     IKDYQGQILKFMGDAILAEFNSVVEAVRCALAIQSELKRVNQTLPEEEKIVIRIGLHV
                     GDVVVAGGDIFGDGVNIAARIQPLAEPGGINITSTVYNQIKNIPGINAVSLGERQLKN
                     IKDPVEILKVPVDNDGALAGEDRRGFSRKTAALLAVAGLLAALGYSGWRHSRISSLPQ
                     NLPPVARNTSTGMETPRPAQELPPAAPLPPTAPPTKTSAAGPSKTIAPAKQPDAAEYA
                     RQAQTFLRDKNFADAVRTLDKAAALDPGNADIFLKRGEALLESGKLDEALKDMSHAIA
                     LRPENPLPYRHRANIQRTKGHTSKALADYEKACGMGDRYSCRMLSKLKGR"
     gene            complement(3273..4034)
                     /locus_tag="P5079_09880"
     CDS             complement(3273..4034)
                     /locus_tag="P5079_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY30328.1"
                     /translation="MTRYLAYSSGLHALLIAFLFLSAAVTKPPRAYYGFQFVGGASGY
                     GPGRLEPAPAPAAPKIASKGTASAKKTPKETARRNDKNKIAVKKEEKTKPSLTQGKPT
                     GKDTAPATAAVPQRGGMPGGRGVSADGRGDRMGSKFGPVGGVGSSLEIGGFGPGGGGE
                     FSARFPYAWYVNILYQKLWKAWNVEDPGTKECTVLFTIARDGSLADAKLQEPSGDSVF
                     DFMALRAVKVSAPFPPLPEGYSDPSLDVVVRFRFQ"
     gene            complement(4031..>4384)
                     /locus_tag="P5079_09885"
     CDS             complement(4031..>4384)
                     /locus_tag="P5079_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRY30329.1"
                     /translation="IDVVLVLLIIFMILSPFLAQSQIPVNLPKAVSSRSVDQDDPIRV
                     QINRQGDFFVGGKRILRNQLEQKLREPLARQPGRAVLIEADRDVSFKNVVFALDTAEQ
                     LEAAKVGVAVLTSNP"
BASE COUNT          875 a         1166 c         1488 g          855 t
ORIGIN      
        1 cggctttccg catggttttg aagagcgtcc cagttgatcg gtccttgggc tgagggaaaa
       61 agaccgtgca gaaacgggta gagggggttg ccgaagaaga gggtgttttt caggagccag
      121 gggagaacga aaagaaaggc cggcccgcag agataaatga gtgtttttcc gaaatcggcg
      181 ggccgccggg ccggatcggc gcgccagtgg acaaaagcgc cgagcgaaag ggcgggcaaa
      241 agagcccatg ccgtgtattt tgtcccgaag gccaccccgc aaaaaaatcc gcacaccaac
      301 agccaattcc tccgctgaag gacgttctcc ttttcgagac ccgtgaggag ggcgaggaga
      361 gccgccaggg agaagaaggc ggtcccgtat tccacggcgg agttggaaaa gaggcgggcc
      421 atcatcggcg gggttgcgaa caaaagcgcg gcggccaggc cggcccgggt ggaggagacg
      481 cggcgcccga cggccagaag gagggccagg cagccgatcc ccagggcata gtggaaaagc
      541 tttgccaggg cgtcgcccga aagggccagc gccagagcgt aaaggagttc cgtgttcatg
      601 ggcgatccgg aaaacaggtt gtgcggcacc gggacgatcc ggtgttccaa aagccaaagt
      661 tgggggagcg ccaggtgata caccaacgaa tcgtagaaag tctcgggcgc cagggtctgt
      721 atcagataga aaagaaggac gaaaagcgtt gagacgacga ggattttgtc caagaggcgg
      781 aaggaagaag tgaacgcggc cgggcgggcg aatcgttccg gggaaaatag ttcctgatta
      841 tcgatgccgg cgagaacgaa gaccgccatg gggatgccca agaggatgac tttataccac
      901 aaccccagca tccccaagac aaagaagagg aggatgaaga gggccgctcc catggcgatg
      961 gaatgaaggg cgttctccag gggagttccc gtggagaggc cgattcggcg caggagggaa
     1021 cgaccggaga gaaaaaaaag gccggcggtc agcgtcgtca ggaaggtttc ccgggccgct
     1081 tccgccgccg cggggagggt cggagggcgc aacagggaga gcatcaggaa gaattgccgc
     1141 gccggcggga attgatggaa gagatagaag ccggagacca ggaggatcca agccgcggac
     1201 agatataaaa cggatttctt cacgggcctt tcaccgtggg agagggcgga tatcagggaa
     1261 gagaggggaa acacgggaga tcgaatcgga cgaggagaat ctgggtccga gggggacatt
     1321 caacgaggac gtgggcgatc catgaaagaa gagcgggggg ggtgtcaggg gcggcggtca
     1381 cttttccccc gttttttttc ggcccacccc ctgccttcgt tcgtaacgca tgaattctct
     1441 gttcagggag cttacgatgt tttggactgt ttgttccgtc cggttttcaa cggcagggga
     1501 atagaggagg ctgagggcca tggtgggcgg aaacggcagg agcgtggtga tccagtgggc
     1561 cagtccgtaa ttgcgtcgat gataggcaga ccaaacgatg tggcccgttt ttacgtcgat
     1621 cagccgggcg ttcacggcgg cctccgccgg cgggaacagc cagtatttcc tcggggagta
     1681 ggaggtcact tctccgatca atacggcgtc cgcgcccaat tcgccggcga tttccaaggc
     1741 ctgttctgtg tcaacccgct gcgaggtcag tcccctgttc ttgatgcggt tttccaccag
     1801 tcctttttca atgatgttgt attgcgggag tttcaggagt tccgtggaaa aaaggtccgc
     1861 cacccggtcg ccaatgccgg ggaatccggc gtcttgaaat gggaagatgg ccaactccaa
     1921 cacctcgttc gcctcaacat gctccttgat gaccagacgc ggcggaagac aggcgggtaa
     1981 aagcgaggtc aaaattatcc ctgcgatcat cagtcgtttg ttcatggcgt tctcccgaag
     2041 tttagttaac gtcctttgag cttgctgagc atccgacaac tgtagcggtc gcccatcccg
     2101 caagcttttt catagtccgc aagcgctttg gacgtatggc ccttcgtccg ttggatgttg
     2161 gcccggtgcc ggtagggcag aggattctcg gggcggagcg cgatggcatg gctcatgtcc
     2221 ttgagcgctt catccagttt ccccgattcc aacagggctt ccccccgttt tagaaaaatg
     2281 tcggcattgc cgggatccag ggcggcggct ttgtccagag tgcgaacggc gtccgcgaag
     2341 ttcttgtcgc gtaagaacgt ttgggcttgc cgggcgtatt ccgctgcgtc gggttgtttc
     2401 gccggggcga tggttttcga ggggcccgcc gccgaggtct tcgttggagg cgccgtcggc
     2461 gggagggggg cggcgggggg aagttcctga gcgggtcggg gtgtttccat gccggtggag
     2521 gtgtttcggg ccactggggg aaggttttgt ggaagagagg atatccggga atgacgccac
     2581 cccgaatatc ccagggccgc caggaggccc gcgacggcga ggagggccgc cgtcttccgc
     2641 gaaaagccgc gacgatcctc gcccgccagg gcgccgtcgt tatccaccgg gactttcaat
     2701 atctcaacgg ggtctttgat gtttttgagc tgccgttctc ccagactcac ggcgttgatg
     2761 ccgggtatgt tcttgatctg gttatagacc gtcgaggtga tgttgatccc cccgggctcc
     2821 gccagaggct ggatacgcgc cgcaatgttg accccgtcgc cgaagatgtc tccgccggcc
     2881 accaccacgt cgcccacgtg aagtccgatg cggatcacga ttttttcttc ttccggaagc
     2941 gtctggttga ctcgtttgag ttcggactgg atggcgaggg cgcaacggac ggcttccacc
     3001 acgctgttga actccgccaa gatggcgtcc cccatgaact tgaggatttg gccctggtaa
     3061 tctttgatga tggccgagag gagggtgttg tgccgctcca acatgcgcaa ggcggcttct
     3121 tcgttggccc ccattttcct gctgaaaccg acgatgtcgg tcagcatgat ggcggccagt
     3181 ttgcgttcgc gtttctgcat ggtcaaaatc ttatcacatt agctgagttt tcgtcgcccc
     3241 gtcggccgac ggggagggcg gcgtggaatg gcttattgga atcggaagcg gacgacgaca
     3301 tccaaagaag ggtcggaata gccttcgggg aggggcggga acggggcgga gactttcacc
     3361 gcgcggaggg ccatgaaatc gaacacggaa tccccggagg gttcctgcag tttggcgtcg
     3421 gccagggagc cgtcccgggc gatggtgaaa aggacggtgc attccttggt gcccggatcc
     3481 tcgacgttcc aggccttcca aagtttctga tagaggatgt tcacatacca ggcgtagggg
     3541 aaccgcgcgc tgaactcccc gccccctccc gggccgaacc cgccgatctc caaggaagac
     3601 cccacgccgc cgacgggccc gaacttcgag cccatccggt ccccgcgacc gtcggcggag
     3661 acgcctcgcc cgccgggcat gccgccccgt tgggggacgg ccgccgtggc cggcgcggtg
     3721 tctttcccgg tcggtttccc ctgggtcaaa gagggtttcg ttttttcttc ttttttgacg
     3781 gcgattttgt ttttgtcgtt tcggcgggcc gtttctttgg gagttttttt cgccgacgcc
     3841 gttcctttgg aggcgatctt gggcgcggcc ggggccgggg cgggctccaa ccgcccggga
     3901 ccgtaaccgg aggcccctcc gacgaactgg aaaccgtaat aggcgcgtgg aggtttcgtg
     3961 acggcggcgc tcaggaataa aaaagcgatg agaagggcgt gcaggccgga ggaataggcg
     4021 agatagcgcg tcatgggttc gacgtcagga ccgccacgcc gactttggcc gcttccagct
     4081 gctcggcggt gtccagggcg aacaccacgt tcttgaaaga gacgtccctg tcggcctcga
     4141 tcagcacggc gcgaccgggt tgcctggcca gaggttctct cagtttttgt tcaagctggt
     4201 tccgtaatat ccgttttcca ccgacgaaaa agtcgccttg ccggttgatc tggacgcgga
     4261 tggggtcgtc ctggtccacg gagcggctgg agacggcctt gggcaggttg accgggatct
     4321 gcgattgggc gaggaagggg gacaggatca tgaagatgat gaggagcacc aggaccacat
     4381 cgat
//
LOCUS       DAOTFD010000211         4359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_14743_length_4359_cov_4.071329, whole genome shotgun sequence.
ACCESSION   DAOTFD010000211 DAOTFD010000000
VERSION     DAOTFD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14743_length_4359_cov_4.071329"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="P5079_09890"
     CDS             complement(<1..178)
                     /locus_tag="P5079_09890"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HRY30330.1"
                     /translation="MNGPVISEARTALEKGDVTPVLKWVKKDDEEEIRRAFKEAWAVR
                     KKGPEAKQMAERYFF"
     gene            complement(327..1058)
                     /locus_tag="P5079_09895"
     CDS             complement(327..1058)
                     /locus_tag="P5079_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRY30331.1"
                     /translation="MKNKLRFITAAVFLSNLFSAAYGEDAPAKKWSEKAQLTYLSANG
                     NTKSSTLGASNEFQYNWTRAALDLTAGALGSQSKEEVTAEQYFAAEKVSWKLTARNYA
                     FERTGWDKNRFAGIANRIDASVGLGRELIKTKRNDLILELGGGYVNEQRVKAPREDFS
                     SGRAYSKYVFAISPQSAFSQDAEYLHNFEHPDGYRVNAETALVSAVSTHMSLKASYLV
                     KYVNQPAPTFGKTDTLTSMALIFNY"
     gene            complement(1083..1508)
                     /gene="mscL"
                     /locus_tag="P5079_09900"
     CDS             complement(1083..1508)
                     /gene="mscL"
                     /locus_tag="P5079_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRY30332.1"
                     /translation="MKIMNEFKEFAMRGNVVDMAVGIIIGGAFGKIVSSFINDVIMPP
                     LGFLIGNADFSDLEITLKAGVDGAKPVVLAYGKFVTVVVDFLIVAFAVFLMVRLMNKM
                     KRAEPAPVAVPTEKKCPECQMLVPIEAKRCGHCTATIAS"
     gene            complement(1578..2009)
                     /locus_tag="P5079_09905"
     CDS             complement(1578..2009)
                     /locus_tag="P5079_09905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY30333.1"
                     /translation="MKRSRAPAPGGKVLIRRARRADVGRIHKLIEEATRSGKILKRSR
                     GEISRLINRFRVAEERGVVVACCALEVYGRKLAEVRSLAVHPERRGRGIASALVAGCL
                     KEARRRRLYEVLAITDRENIFKRLGFAEQLHGQKALFVRPS"
     gene            complement(2006..3466)
                     /locus_tag="P5079_09910"
     CDS             complement(2006..3466)
                     /locus_tag="P5079_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY30334.1"
                     /translation="MPKMKINEKVLQKTIKLCKEKNIVIPTFEQLAQPAKTPESVQKK
                     MKSVGLWDVNPVNLFRITWKNNVKTGLFGDVNAIEIPPALSGVDARIVGLVGKWFPTG
                     AHKVGAAFGCLAPRLVSGEFDPTTQKAVWPSTGNYCRGGAFDCALLSCTAVAILPQEM
                     SKERFTWLKSIGAEVIATPGCESNVKEIYDKCWEIRKTRKDCVIFNQFDEFGNPIWHY
                     YCTGNALEEAFNKIKKKGDRFSGYVSATGSAGTIGAGEYLKKIHPHVRLGASEALQCP
                     TLLRNGFGGHRIEGIGDKHVPWIHNVRNTDMIIDINDENCMRTLRLFNEPAGKKVLRQ
                     NGVSAELIEQLPLLGISSISNLLSAVKMAKYYEFNKNDVVFTIFTDSAEMYGSRLEEL
                     RKDRGAYTDLQAAIDHERYVKGTATDDLKELSYYDRKALHNLKYFTWVEQQGKTVEEL
                     NRLWEPDFWDETFEQVHEWDKLIVEFNKATGLAKTA"
     gene            complement(3802..4299)
                     /locus_tag="P5079_09915"
     CDS             complement(3802..4299)
                     /locus_tag="P5079_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRY30335.1"
                     /translation="MLNRKIQEEFNKQINEELASAYIYLSMAAYFESQNLKGMAGWMR
                     VQAKEEQEHAMKFYGFINERGGRVQLESIAGPKTDWKSPLSAFEDAHKHEIHITGRIN
                     GLLELARKERDSAAEIFLHWFVSEQVEEESNVTEIVAKLKMIGESSSGLLYLDKELGK
                     RGSKD"
BASE COUNT          830 a         1354 c         1129 g         1046 t
ORIGIN      
        1 cgaaaaaata ccgttccgcc atctgtttcg cttccggccc cttcttccgg acagcccagg
       61 cctccttgaa agcccgtcga atctcctcct catcgtcttt cttgacccac ttgaggaccg
      121 gagtgacgtc gcccttttcc aacgccgtcc gggcttccga aatcacaggc ccattcatgg
      181 tatcacaatg cgcccgaagc aatggacctg tcagcatcac ccccaaaccg acggcgctga
      241 ttcggacgca cgttttgaac actctcattt tcatcccccc gtcggcggtc ttccgcccat
      301 catttttctc cggccccagc gacagctcaa tagttgaaaa taagggccat cgaggtcagc
      361 gtatcggtct tgccgaacgt cggcgccggt tggttcacgt atttgaccag gtaggacgcc
      421 ttcaaagaca tgtgggtcga cacggcggac accagcgccg tctcggcgtt gacgcggtag
      481 ccgtcgggat gttcgaagtt gtgcagatat tcggcgtctt gcgaaaacgc ggactgcggg
      541 ctgatggcga acacgtattt cgaataggcg cgtcccgaag aaaagtcttc cctcggcgct
      601 ttcacgcgct gctcgttcac atagccgccg ccgagttcca agatgaggtc gtttctcttc
      661 gtcttgatga gctcccgccc aagaccgacg gaagcgtcaa tgcggttggc gatgcccgca
      721 aagcggttct tgtcccaacc ggtccgctcg aaagcgtaat tccgcgccgt cagcttccag
      781 gacacttttt ctgcggcaaa atactgttcc gccgttacct cctccttgct ctgggaaccc
      841 aaggcgccgg cggtcaaatc gagcgccgca cgggtccagt tgtattggaa ctcgttggag
      901 gcccccaacg tggaggattt tgtgttcccg ttggcgctca aataggtcaa ctgcgccttt
      961 tccgaccatt tcttggccgg cgcgtcctcg ccgtaagcgg cagaaaacag attcgacagg
     1021 aaaacggcgg cggtgatgaa acggagcttg ttcttcacgg tctctccttt tgtctctatt
     1081 tttcaggaag cgatcgtcgc ggtgcaatgt ccgcaacgtt tcgcttcgat ggggacgagc
     1141 atctgacatt ccgggcattt tttctcggtg ggaaccgcga ccggtgcggg ttcggcgcgc
     1201 ttcatcttgt tcatgaggcg caccatgagg aaaacggcaa aagcgacgat gaggaaatcc
     1261 accacgacgg ttacaaactt cccgtacgcc aggaccaccg gcttggcccc gtcgaccccg
     1321 gcctttaagg ttatctccag atcggaaaaa tccgcattcc cgatcaggaa gccgagcggc
     1381 ggcatgatca cgtcgttgat gaaggaggac acgatcttgc cgaaagcgcc gccgatgatg
     1441 atccccaccg ccatgtccac cacgttcccg cgcatggcga actctttgaa ttcattcatt
     1501 atcttcatgt tcccctcctt ccaaattttt tgacatggcc gcccggctca aaactccgcg
     1561 ccgacagagg aaacccctta cgacggtctc acaaaaagcg ccttctggcc gtggagttgc
     1621 tctgcaaatc ccaaccgttt gaaaatattc tcccggtcgg tgatggcaag cacctcatag
     1681 agcctccggc gacgcgcctc tttgaggcaa ccggccacca gagcgctggc gatgccccga
     1741 ccgcgacgtt ccgggtggac cgccaaagaa cggacctccg ccagtttgcg gccgtagacc
     1801 tccagagcgc aacacgccac aacgacgccc cgctcctccg cgacccggaa acggtttatc
     1861 agtctcgaga tttcgccgcg ggatcgcttg agtatctttc cgcttcgggt ggcttcttcg
     1921 atgagtttat ggattcggcc gacgtccgcc cggcgcgccc ggcggatgag gaccttcccc
     1981 cccggcgcgg gggcccggct tcgcttcacg ccgttttcgc caatccggtg gccttgttga
     2041 attccacgat cagcttgtcc cattcatgga cctgttcgaa cgtttcgtcc caaaaatcgg
     2101 gttcccagag acggttgagt tcctcgacgg tcttcccctg ctgttccacc caagtgaaat
     2161 atttcaggtt gtgcagggcc ttccggtcgt aatacgaaag ttccttcagg tcgtccgtcg
     2221 ccgtgccctt cacgtaacgt tcatggtcga tggccgcctg gaggtccgtg taggcgccgc
     2281 ggtctttcct cagctcttcc agccgcgacc cgtacatttc cgccgaatcc gtgaagatgg
     2341 tgaacacgac gtcgttcttg ttgaactcgt aatatttggc catcttgacg gccgacagca
     2401 ggttgctgat gctggagatg ccgagcagcg gcagctgttc gatgagttcc gccgaaacgc
     2461 cgttctgcct cagcaccttc ttgccggccg gctcgttgaa aaggcgcaag gtcctcatgc
     2521 agttttcatc gttgatgtcg atgatcatgt ccgtgttgcg cacgttgtgt atccagggga
     2581 catgtttgtc gccgatccct tcgatccgat gtccgccgaa cccgttgcgc agaagggtcg
     2641 ggcattgcag cgcttccgac gctccgagcc ggacatgggg atggattttc ttgaggtatt
     2701 ctcccgcccc gatggtgccc gcggacccgg tggccgaaac atagccggag aaccggtcgc
     2761 cctttttctt gatcttattg aaggcctctt ccagcgcgtt gccggtgcag taataatgcc
     2821 agatgggatt cccgaactcg tcgaactggt tgaagatgac gcagtccttc cgcgtcttcc
     2881 ggatctccca acacttgtcg tagatctcct tgacgttgct ttcgcaaccc ggggtggcga
     2941 tcacttccgc gccgatggac ttcagccagg tgaaccgttc cttgctcatc tcctggggca
     3001 ggatggcgac ggcggtgcag gacaagaggg cgcaatcgaa ggcgccgccg cggcaatagt
     3061 tgccggtgga cggccagacg gccttttgcg tcgtcggatc gaattccccg gaaaccagcc
     3121 ggggggcgag gcaaccgaaa gcggccccca ccttgtgcgc cccggtgggg aaccatttgc
     3181 cgacgagccc gacgatccgc gcgtcgacgc cggagagggc cggggggatc tcgatcgcgt
     3241 tgacgtcccc gaacagtccc gtcttcacgt tgtttttcca cgtgatcctg aaaaggttca
     3301 ccgggttgac gtcccacaac ccgacgctct tcattttttt ctggacggat tcgggcgttt
     3361 tcgccggctg agccagttgc tcaaaagttg ggatgacgat gttcttctct ttgcacagtt
     3421 taatggtttt ctgaaggacc ttttcgttga ttttcatctt tggcattttc cgatgtctcc
     3481 ccatgcttta tttttatgcg ctccccgcaa attcaaaaac gtccgacctt gatcttcctt
     3541 tcacgccgcc ccgcccgtcg gcaccacagg caaggcctca cattatactc acctgggaga
     3601 aactatcaaa tgcagaaaac cttcccggtc aaagccccgc ccttccaaaa ttcaaggcat
     3661 ccggcccaca aaacgaaagc ctcccgaaat ccagccggag ccggactctt gggaggcttt
     3721 gcgtgcctct aattcaaccg atggccgcgc ctatttctcg cttacaacca accgcttaga
     3781 tgaacatccc cttcgggttc ttcaatcctt cgatccccgt ttccccaatt ctttatccag
     3841 atacagaagt ccgctggagc tctccccgat catcttcaac tttgcgacga tctcggtcac
     3901 gttggattcc tcctccacct gttcgctcac aaaccaatgg agaaaaatct ccgcggccga
     3961 atccctttcc ttccttgcca attccaacag gccgttgatg cgccccgtga tgtggatctc
     4021 gtgtttatgg gcgtcctcaa aagcggaaag gggcgatttc cagtccgttt ttggaccggc
     4081 aatcgattcc agctgcaccc ggccgccgcg ttcgttgatg aatccataaa acttcatggc
     4141 gtgttcctgt tcctctttgg cctgaaccct catccagccg gccatccctt tgagattttg
     4201 agactcaaag taggccgcca tggagagata gatgtaggcg gaggccagct cctcattaat
     4261 ctgtttatta aactcttcct ggattttcct gttcaacatg ggtttgtccc ctcacttttt
     4321 atttttcatt tatctcgaca tttttccggc cgtttcccg
//
LOCUS       DAOTFD010000212         4339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_14848_length_4339_cov_5.070495, whole genome shotgun sequence.
ACCESSION   DAOTFD010000212 DAOTFD010000000
VERSION     DAOTFD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14848_length_4339_cov_5.070495"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5079_09920"
     CDS             <1..220
                     /locus_tag="P5079_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRY30336.1"
                     /translation="AGRAKPGDPVDHSVGIEFHKKTGDIVRLGESLATVHAGSREKLE
                     EAQRLYQEAVVLGPRRPPKQPLVYGLIR"
     gene            249..1130
                     /gene="mtnP"
                     /locus_tag="P5079_09925"
     CDS             249..1130
                     /gene="mtnP"
                     /locus_tag="P5079_09925"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRY30337.1"
                     /translation="MSKKVLKRESVKVAVIGGSGLYDIEGIEDVKEIRLKTPFGEPSD
                     TIVVGRLRGVPCAFLPRHARGHRLLPTELPARANIYALKSLGVEQIVGVGACGSLKEE
                     IPPRDFVVPDQIYDRTKGRHSTFFGDGVVAHVTFDQPYCPELSSLLADGVEKIGVRVH
                     RGGTYVCMEGPAFSTKAESEVNRRLGFSVVGMTALPEAKLAREAEICYSTVALVTDYD
                     VWKEGEEVSVGKLVENLRANVENVKKTLASALPLLARRTRRCRCASALQYAIFTDPKA
                     MNPGTKKKLNLLIGKYV"
     gene            1214..1831
                     /locus_tag="P5079_09930"
     CDS             1214..1831
                     /locus_tag="P5079_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30338.1"
                     /translation="MIGRPGSGKSEFIRRAARRLTEEGTVRRTCRVDDCSKLWDIFRL
                     EESSGEWKRCRKMPGGGYRVTDPSVWDELLRAVAREVGELETSSAATFVEFSRASYAA
                     AFKNFSPDLLRRSVVAYVDCSFDTCWRRNLQRSKSSEGQDRHFVSREEMESTYRRDDR
                     EEFLRQSPAPVFVVDNESDGTEHLQAAAEVFAGKLRDVGKAGGTS"
     gene            1828..2895
                     /gene="add"
                     /locus_tag="P5079_09935"
     CDS             1828..2895
                     /gene="add"
                     /locus_tag="P5079_09935"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRY30339.1"
                     /translation="MTDIVQNAENEVLKKLPKVDLHLHLDGAARPETLMDLMRASGAS
                     LPTEDPREFQKYVQVPRSCRSLADFLKAFEFFYPFLKSPEAVERLAYELCEDCSRLNV
                     HCFEARFAPALQAKARLSQEDILRAALRGVERGARDFSVKAGLILCCYRSIPQTENDE
                     TVELAEKYLGRGVVGIDLAGDESRYPVSLYAACFKRAVERHIPITCHAGEADGPKSID
                     NALHLGARRIGHGVRLQEDSGVFERVKRERVPLEMCITSNVQTQAVKGFAVHPAKRYL
                     EAGLCVTLNTDDPGVSGIDLVHEYAVAMGKLNFTERDLVRVILNGVDSLFLPEPEKKE
                     LRRRYEKELASLLKLPTGERL"
     gene            2892..3317
                     /locus_tag="P5079_09940"
     CDS             2892..3317
                     /locus_tag="P5079_09940"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRY30340.1"
                     /translation="MKNKNKKPLTAVYRRLLAAAKAARQKAYAPYSRYRVGAAVLTFG
                     GKIHAGCNVENASYGLTCCAERVAIFKAVSVGERRLRAVAIVLDAPSYGAPCGACRQV
                     IYEFGPAADVLMGTVRGGVRVQRIGELLPLAFGPAHLKD"
     gene            3324..>4339
                     /locus_tag="P5079_09945"
     CDS             3324..>4339
                     /locus_tag="P5079_09945"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HRY30341.1"
                     /translation="MTSSPAEQESTGTRRGKTVALKDGSVHLLVGSASFRVEKGVLSS
                     AGARLPAQTSVRVPFGRALPLEAEEPSEIVLFDGSETRLRDVGRRTVPEAWTAAADAL
                     VKDAPRLLLCLGFSYTGKNFFCTHLANRFRAAGKRVCVIDADPDQTEIGPPGCVGLLD
                     MNDPVLFFSQALPTALQYVGSHSVIARPAVVLAAAQRLVNKALHERRAEVVLVNVPGW
                     TAEDSGRCFHALLSTLLSPDRTIVLQRKDESEHLVRGVPVSRTVRLRASEFAARSSAD
                     ERKRYREERTTAYYQNRRLLVLPFHHVSSAGASFLSGYPQKIVNEKILYVERMPSELE
                     GVLVA"
BASE COUNT          768 a         1341 c         1408 g          822 t
ORIGIN      
        1 cgccggccgc gccaaacccg gcgacccggt ggaccattcc gtggggatcg agttccataa
       61 gaagaccggc gacatcgtcc ggttggggga gtccctggcg acggtccacg ccggttcccg
      121 ggagaaactg gaagaggcgc aacggctcta tcaagaagcg gtggtcctcg gcccgcgccg
      181 tccccccaaa caaccgcttg tctacggcct tatccgttga gatatccgcg cttccgggag
      241 gagtgaaaat gtccaaaaaa gttttgaaac gggagtccgt gaaggtcgcc gtcatcggcg
      301 gcagcgggtt gtacgacatc gaagggatcg aggacgtcaa ggaaatccgt ctgaaaacac
      361 ccttcgggga accgtccgac accatcgtgg tgggccggct gcgaggcgtc ccctgcgctt
      421 tcctgccgcg gcatgcgcgg ggacaccgcc tcctgcccac cgaactcccc gcccgcgcca
      481 acatctatgc gctcaagtcc ctcggcgtgg agcagatcgt cggggtcggc gcctgcggca
      541 gtttgaagga ggagattcct cctcgggatt tcgtggtccc ggatcagatc tacgaccgca
      601 ccaagggccg gcactcgact tttttcgggg acggcgtggt ggctcacgtg acgttcgatc
      661 agccctattg cccggaactt tcttccctct tggccgacgg cgtggaaaag atcggggtgc
      721 gggtgcaccg gggcggaacc tacgtgtgca tggagggacc ggctttttcc accaaagccg
      781 aatccgaggt caaccgccgg ctgggttttt ccgtcgtcgg catgaccgcc ctgccggaag
      841 ccaaactggc ccgcgaggcg gagatctgtt attccacggt ggccctcgtc accgattacg
      901 acgtttggaa ggagggggag gaggtcagcg tgggaaagct ggtcgagaac ctccgcgcca
      961 acgtggagaa cgttaaaaag acgctggcgt cggctctgcc gctcctggct cgtcggacgc
     1021 ggcgctgccg gtgtgcttcg gcgctgcaat acgcgatttt tacggacccc aaggccatga
     1081 accccggcac caaaaagaag ttgaacctgc ttattggaaa atacgtctag tgcccggcgc
     1141 ccggccggat attgaaacaa gccgtccctt gaacgaaccg gcccgccgga gccgggaagg
     1201 cctttttata tttgtgatcg gccgccccgg cagcgggaaa tcggagttca tccgccgggc
     1261 ggcccggcgt ctcacggagg aggggacggt ccgccggacc tgtcgcgtgg acgattgctc
     1321 gaagttgtgg gatattttcc gcctggaaga atcgtccggg gaatggaaac gctgtcgaaa
     1381 gatgcccggc ggcggatatc gtgtgaccga cccttcggtg tgggacgagc tcctccgcgc
     1441 cgtggcgcgg gaggttgggg aattggaaac ctcttccgcc gcgaccttcg tggagttctc
     1501 gcgggcctcc tacgcggccg cttttaagaa tttttcgccg gacctgttgc ggcggtcggt
     1561 ggtggcgtat gtggactgtt cctttgatac ttgctggcgc cggaacctgc agaggtcgaa
     1621 gtcttccgaa ggacaagacc ggcatttcgt cagccgggag gagatggagt ccacctatcg
     1681 ccgggacgat cgggaggaat tcctccgtca atcgccggcg ccggtgttcg tggtcgataa
     1741 cgaatctgac ggcacggaac atttacaggc cgcggccgag gtttttgcgg ggaaactgag
     1801 ggacgtaggg aaggcggggg gaacgtcatg acggacatcg tgcaaaacgc cgagaacgaa
     1861 gttttgaaaa aactgcccaa ggtggacctg catctccatt tggacggcgc ggcccgcccg
     1921 gagacgctca tggacctgat gcgcgcctcc ggcgcgtctc tgcccaccga ggatccgcgg
     1981 gaattccaga aatacgtgca ggtcccgcgc tcctgccgca gcctggccga tttcctgaag
     2041 gctttcgagt ttttttatcc gttccttaaa tcgcccgaag cggtggagcg cctcgcctac
     2101 gagctctgtg aggattgttc ccgtttgaac gtccactgtt tcgaggcgcg gttcgccccg
     2161 gcgctgcagg ccaaggcgcg actctcgcag gaggatatcc tccgcgccgc ccttcgcggc
     2221 gtcgaacggg gcgcccggga tttctccgtc aaggccggat tgatcctctg ttgctatcgc
     2281 tccatcccgc agacggaaaa cgatgagacg gtggagctgg ctgaaaaata tctcggccgc
     2341 ggcgtggtgg ggatcgacct ggcgggagac gagtcccgct accccgtctc gctgtatgcc
     2401 gcctgtttta agcgtgccgt ggaacggcat attcccatca cctgccatgc cggcgaggcg
     2461 gacggcccga aaagcatcga caacgccctt catttggggg cgcggcggat cggccacggg
     2521 gttcgcctgc aggaggattc cggcgtcttc gagcgcgtga aacgggaacg cgtccccctg
     2581 gagatgtgca tcacctccaa cgtgcagacc caggcggtga aagggttcgc cgtccatccc
     2641 gccaagcgtt atctggaggc cggcctgtgc gtgaccctca acaccgacga tcccggggtc
     2701 tccggcatcg atttggtgca cgaatacgct gtggccatgg gaaaattgaa tttcactgaa
     2761 agagatttgg ttcgggtcat cctgaacggg gtggacagct tgttcctgcc ggaaccggag
     2821 aagaaggaac tgcgccggcg ctacgagaag gaattggctt ccctcttaaa attaccgacg
     2881 ggagaacgcc tttgaagaat aaaaacaaga aaccgctgac cgccgtttac cgccgtcttt
     2941 tggcggcggc aaaagcggcg cgacaaaaag cctacgcgcc ctattcccgc taccgcgtgg
     3001 gggccgcggt cctgaccttc ggcgggaaga ttcacgccgg gtgcaacgtg gaaaacgcct
     3061 cctacggcct gacctgctgc gcggaacgcg tcgccatctt caaggcggtg tccgtcggcg
     3121 aacgccgcct gcgggccgtg gccatcgtcc tggacgcgcc ctcctacggc gccccctgcg
     3181 gcgcctgccg ccaggtgatt tatgagttcg gccccgccgc cgacgtcctg atgggcaccg
     3241 tgcggggggg agtgagagtc cagcgcatcg gcgaactcct gccgctcgcc ttcggccccg
     3301 cccacctcaa agactgatcc cgcatgacgt cttctcccgc cgaacaagaa agcacgggga
     3361 cccgtcgagg gaagaccgtc gcccttaaag acggttccgt gcatcttctg gtcggctccg
     3421 cgtcgttccg cgtggagaaa ggcgttctct cttccgcggg ggcgcgcctt cccgcacaaa
     3481 cctccgtccg cgtcccgttc ggccgggcgc tgccgctgga ggcggaggaa ccgtccgaga
     3541 tcgttctgtt cgacggcagc gagacccgcc tgcgggacgt gggccgccgc acggttccgg
     3601 aagcgtggac cgccgccgcg gacgctttgg tgaaggacgc cccccggctt ctcctgtgcc
     3661 tcggtttttc ctacacgggc aagaattttt tctgcaccca tctggccaac cgttttcggg
     3721 cggccggaaa gcgggtctgc gtgatcgacg cggacccgga tcagacggag atcggtccgc
     3781 cgggctgcgt gggccttctg gacatgaacg accccgtgtt gttcttttcg caggcgcttc
     3841 ccacggccct ccaatacgtg gggtcccatt cggttatcgc gcggccggcc gtggtcctgg
     3901 cggcggcgca gcgtttggtg aacaaggcgc ttcacgagcg tcgggcggag gtcgtgcttg
     3961 tcaacgttcc cggctggacg gccgaagaca gcggccgctg tttccatgcc ctcctttcca
     4021 ccctcctttc gccggatcgg acgatcgtcc tccaacgcaa ggacgagtcc gagcatttgg
     4081 tccggggcgt ccccgtgtcc cggacggtgc ggttgcgggc gtccgaattc gcggcgcgaa
     4141 gttcggcgga cgaacggaaa cgatatcgcg aggaacgaac gacggcctat tatcagaacc
     4201 gccggctgct cgttttgccg ttccatcacg tatcgtcggc gggcgcgtcc ttcctttccg
     4261 gatatccgca aaagatcgtc aacgagaaaa tcctttacgt cgagcggatg ccgtcggaac
     4321 tggagggggt cctggtggc
//
LOCUS       DAOTFD010000213         4267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15176_length_4267_cov_4.486942, whole genome shotgun sequence.
ACCESSION   DAOTFD010000213 DAOTFD010000000
VERSION     DAOTFD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15176_length_4267_cov_4.486942"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="P5079_09950"
     CDS             complement(<1..1003)
                     /locus_tag="P5079_09950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30342.1"
                     /translation="MMKESPRSLKQLWLLLFVVALATRLFIFAAFHYKWNVLGSFAKG
                     AVATDFEGDFWEPAQHIAHGDGCIVLYNFRLQPTAYYPPGQVYLYAGLYRLFPGRHLL
                     AAKILQTLFVSLAFVLFGVYIEALFGRRVAWAAFALMNLSPFLGIAHLAYTVNSSALL
                     LSAVFLVRLESFLKRPTYGGSSELGVWFGLMLLFNSSYIALGLAGVPWLLWKAKVSWK
                     HAVLVSVVAATLLLPWAVRNYRVFGKFIPLRSGFKETFYYGNYPRATGGTREIDLKTN
                     TEPMLELREKIVGKNEVEVGDILFVEALKFIREQPLRFVRVRLNAWLYFWTTEAYWIF
                     "
     gene            1396..2328
                     /locus_tag="P5079_09955"
     CDS             1396..2328
                     /locus_tag="P5079_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY30343.1"
                     /translation="MIEIQNLVKHYGDTKAVDDVSFEVRPGEVVGLLGPNGAGKSTTL
                     RVLTGYLPPTHGTVRLGGMDIQSDPLAARKQVGYLSESNPLYESLGVWECLDIVARLR
                     GLPADSIARRVTQVVAECSLQDVVSKDIGALSKGYRQRLGLALAILHDPDILVFDEPS
                     SALDPNQQKEVRDLILKLKEKKTVLLSTHILPEAQVLCDRLLIIHRGKIVAQGTVAEL
                     QGQAAGEKTFYVRLAGPAREIEPSLRSLPGLTRVEFADEQEAGCPAYRVSGAGDPRGA
                     LFQLASERRWPLMELRREAASLDEIFRMLTGNPS"
     gene            2325..3041
                     /locus_tag="P5079_09960"
     CDS             2325..3041
                     /locus_tag="P5079_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY30344.1"
                     /translation="MNLTAIRVLIRKELKESFNSPMPYIFLTVFFLVAGWFYVSSLFL
                     MGQATLEDFFSPMPLLLVFFMPAFTMRLFSEEYKSGTVELLSTLPLRDTEIVLGKYLA
                     ALAVWALMLGMSLFYQGLLVAVGRPDIGQSLTGYFGAFLLGAFYLAAGLFASSLTRSQ
                     VVAFLLGFFFAIFFFLCGKAAHMVPGIWGTGLTFLGVDAHYESFLRGVIDTRDVVYYL
                     SGLVLFLGATLAGFNSRRWR"
     gene            3050..>4267
                     /locus_tag="P5079_09965"
     CDS             3050..>4267
                     /locus_tag="P5079_09965"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRY30345.1"
                     /translation="MPDLRAFFRERKLRLGVLSGSGVLVLAGILAAANLLSQTLFTRW
                     DWTADHRYSLSPASKKLVRSLPDPLLLRFYLSPGLPQPYETQGRYLRDLLGEYRAAAK
                     GKIRLEILNPEESEKTKAEATRAGVAPVRFTQVASDQFQIREAFMGLVLFYQDKQEVV
                     PFIKDTQTMEYDISSRIKKMSAAKKRALGLVTGHGEVSLYELQPEGSTLFEGFDAQPV
                     QLSTESPVNADVLLILGPRQPFSPTDLKILDGYLARGAPLIITLSRQMLNLRSFTSLV
                     QKTELEPFLQHYGVRLETDVVLDAQCQRITLQSQQGQFSVANILNYPAIPLSNRLDKN
                     NPVLRTVDALGFPFAHPLLPVDGQSTVRFTPLAESSPNSWISPGLSRFDPYNIPGPGE
                     NSRKGPFVLAALVE"
BASE COUNT          897 a         1399 c         1168 g          803 t
ORIGIN      
        1 ggaaaatcca gtaggcctcg gtggtccaaa aatacaacca ggcgttcaac cggacccgca
       61 caaaccgcag aggctgttca cggatgaact tcagcgcctc cacgaacagg atgtcgccca
      121 cctccacctc attttttccg acgatctttt cccgcagctc aagcatcggc tcggtgttgg
      181 ttttcaggtc gatctcccgg gtccccccgg tggcgcgggg ataattcccg taataaaaag
      241 tctctttaaa gcccgagcgg agcgggataa acttcccgaa cacccgatag ttcctcacgg
      301 cccacggcag caacaacgtc gccgccacca cgctcaccaa caccgcgtgt ttccaggaga
      361 ctttcgcctt ccacaggagc cacgggacgc ccgccaaccc gagcgcgatg taggacgaat
      421 tgaagagcag catcaggccg aaccaaacgc ccagttcgga ggaaccgccg taggtgggcc
      481 ttttgagaaa cgattccaag cgcaccagaa aaaccgccga caacaggagg gcgctggagt
      541 tgacggtata ggccaaatgc gcgatgccca aaaaggggct caggttcatc agcgcaaacg
      601 ccgcccaggc cacgcggcgg ccgaacagcg cttcgatata aacaccgaaa agaacgaagg
      661 cgagactcac gaacaaggtc tgcaggatct tcgccgccag aagatgccgt ccgggaaaca
      721 accggtaaag cccggcgtag agatagacct gccccggcgg gtaataggcg gtcggctgaa
      781 gacgaaaatt gtagagaacg atgcagccgt ccccgtgggc gatgtgttgg gcgggctccc
      841 aaaaatcccc ttcaaaatcc gtggccaccg cgcccttggc gaaagatccc aaaacgttcc
      901 atttatagtg gaaggccgca aaaataaaca gccgggtggc caacgccacg acgaaaagaa
      961 ggagccacag ctgttttaaa gaacggggcg attctttcat cattagacga aaaagtatac
     1021 aaaaatcagc ccccgcctcc tagcgagctc ctcggaaagt cccccaaaaa aacgacggga
     1081 acatgccgca ggggggcgga caatgttccc gtctaaatcc ttcccgaatc cgtgggcccc
     1141 acaacaaaac gggtcccgtt cctttcgaaa cgggaccgcg tcgtcccggg gactccccgg
     1201 aaaacaaaaa tcctccccaa gggttcaggg aggacctcag tgtccggaaa aaatgctcgt
     1261 ccggcacgtc cttgtgccgt ttttaaatgc taccacgccg ggccgcccct gtcaagttcc
     1321 gcaaaagacg ccggggaccg gatttgaaat gccggtggcc agtattatag aatctccccc
     1381 taagaaattt catccatgat cgaaatccaa aacctcgtca aacattacgg cgacaccaaa
     1441 gcggtggacg acgtctcctt tgaggtccgg cccggcgaag tggtgggcct tctgggtccc
     1501 aacggcgccg gcaagagcac caccctgcgc gttctgaccg gctatctccc ccccacccac
     1561 gggacggtcc gcctgggagg gatggacatc cagtcggacc ccctcgccgc gcgaaaacag
     1621 gtgggctacc tctccgaatc caatcccctg tatgaatcgc tcggggtatg ggaatgcctg
     1681 gacatcgtgg cccgcctgcg ggggcttccg gcggactcca tcgcccgtcg ggtgacgcag
     1741 gtggtggcgg aatgttcgct ccaggacgtc gtctcgaaag acatcggcgc gctttccaaa
     1801 ggctaccgcc aacgcctcgg cctggcgctg gccatcctac acgacccgga catcctcgtc
     1861 ttcgacgaac ccagctcggc gctggacccc aaccagcaaa aagaggtgcg ggacctgatc
     1921 ttgaagctca aggagaaaaa aacggtcctt ctttccacgc acatccttcc ggaggcccag
     1981 gtcctctgcg accggttact catcattcat cgcgggaaaa tcgtggccca gggaaccgtg
     2041 gccgaactgc agggccaagc cgccggcgaa aaaacctttt atgtccggct cgccggccca
     2101 gcccgggaaa tcgagccgtc gctccgttcc ctgccgggtt taacccgggt ggagttcgcc
     2161 gacgaacagg aggcgggatg tccggcctac cgggtgtccg gagccggaga ccctcgaggg
     2221 gcgctcttcc aactggcctc ggagcggcgc tggcccctga tggaacttcg ccgcgaggcc
     2281 gcctccctgg acgagatatt ccgcatgctg accggaaacc cctcatgaac ctcaccgcca
     2341 tccgcgtgct catccgaaaa gagttgaaag aatccttcaa ctcccccatg ccgtacattt
     2401 tcctgacggt ctttttcctt gtcgccggtt ggttctatgt gtcgtccctg ttcttgatgg
     2461 gtcaggccac cctggaagac ttcttctcgc cgatgccgct tctcctggtg ttcttcatgc
     2521 cggcgttcac catgcggctg ttttccgagg agtacaagtc cggcacggtg gaacttcttt
     2581 ccaccctgcc cctgcgcgat acggagatcg tcctcggtaa atacctggcc gccctggcgg
     2641 tgtgggcgct gatgctgggg atgtcgttgt tctaccaggg ccttctggtg gccgtgggcc
     2701 ggccggacat cggccaatcg ctcaccggtt acttcggggc gttcctcctg ggcgccttct
     2761 acctggcggc cgggcttttc gcatcctccc tgacgcgcag ccaggtggtg gccttccttc
     2821 tggggttctt tttcgccatc ttctttttcc tgtgcggcaa ggccgcgcac atggtgccgg
     2881 gaatatgggg gacggggctg accttcctcg gcgtggacgc ccattacgaa tccttcctgc
     2941 gcggcgtcat cgatacccgc gacgtggtct attacctttc cggactggtc ctgttcctgg
     3001 gggcgacact ggccgggttc aacagcaggc gctggcgatg aaatcgagaa tgccggacct
     3061 tcgcgcattt ttccgagaaa gaaaactccg actaggggtc ctgtccggca gcggcgttct
     3121 cgtcctcgcc ggcatcctcg cggcggccaa tctcctttcc caaacgcttt tcacccgctg
     3181 ggactggacc gccgatcacc ggtattcctt atcgccggcg agcaaaaaac tcgtccgctc
     3241 tctgccggat ccactcctcc ttcgtttcta tctctcgccg ggactgcctc agccttacga
     3301 aacccagggc cgctacctcc gcgacctttt aggcgaatac cgcgccgccg ccaaagggaa
     3361 aatccgtctg gaaattttga atccggagga atcggagaaa acaaaagccg aggccacccg
     3421 cgccggagtg gcccccgtcc gcttcaccca ggtggccagc gaccagttcc agatccggga
     3481 ggcgttcatg gggctggtcc tcttctatca ggacaaacag gaggtcgtcc cttttataaa
     3541 agacacccaa acgatggaat acgacatctc cagccgcatc aagaaaatgt cggccgcaaa
     3601 gaaacgcgcg ctggggctgg tcaccgggca cggggaggtg agcctctacg aacttcaacc
     3661 cgagggctcg acgctcttcg aagggttcga cgcgcaaccc gtccaactga gcaccgaatc
     3721 cccggtcaac gccgacgttc tcctgatcct cgggccgagg cagccctttt ctccgacgga
     3781 cttaaaaatc ctcgacggct atctggcccg cggcgcgccg ctcatcatca ccctcagccg
     3841 ccagatgctg aacctccgga gtttcacctc tctcgttcag aaaacggaac tggaaccgtt
     3901 cctccaacat tacggcgtcc ggcttgaaac cgatgtggtg ctggacgccc aatgtcagcg
     3961 catcacgctc caatcgcaac agggccaatt ctccgtcgcc aacatcctga actatccggc
     4021 gatcccgttg agcaaccggc ttgacaagaa caacccggtg cttcggacgg tggacgccct
     4081 cggattccca ttcgcccatc ccctcctccc cgtcgacgga caaagcacgg tccggttcac
     4141 tccgctggcc gaatcgtcgc cgaacagctg gatctctccg ggcctttccc gcttcgaccc
     4201 ctacaacatc cccggcccag gggaaaattc acgcaaaggt ccgttcgtct tggcggcctt
     4261 ggtggag
//
LOCUS       DAOTFD010000214         4239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15333_length_4239_cov_4.666826, whole genome shotgun sequence.
ACCESSION   DAOTFD010000214 DAOTFD010000000
VERSION     DAOTFD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15333_length_4239_cov_4.666826"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="P5079_09970"
     CDS             <1..463
                     /locus_tag="P5079_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRY30346.1"
                     /translation="LLKGPTFAETMKVFRDPRLRVSVQGDRPEDHDTPTPLCEAGRSY
                     VALGPNGDVYPCLQWRAVLGNVRRNALKDIWNSEEARRRRAMTRADYAVCNACDLLAY
                     CPRCTGVAEREDGDFLGPSAAACRLARLNRDVLVGRSRSGAERPAAEAHPL"
     gene            483..626
                     /locus_tag="P5079_09975"
     CDS             483..626
                     /locus_tag="P5079_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30347.1"
                     /translation="MSKKKKPYKKPTLLSEKIIEQAALGCKKCTSGNPIVQARCSAQL
                     SSS"
     gene            677..994
                     /locus_tag="P5079_09980"
     CDS             677..994
                     /locus_tag="P5079_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30348.1"
                     /translation="MRPFLSSRSRLVPLLKTAVCRAGDVALCGEPDRAVLHRLYRRQG
                     NGWWAADDAARTPFQWVPDGALRGRLLGHTPQGTPGLLWGYAARILFSAARRFKRLFN
                     VSS"
     gene            994..1755
                     /locus_tag="P5079_09985"
     CDS             994..1755
                     /locus_tag="P5079_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY30349.1"
                     /translation="MDLVVSHAFKQFPSSAGPKKILKDISFRVTAGEVVCLLGPNGSG
                     KTTLLKTIATLLSPDGGEVLLDGKSVHSDTLRTRRLLGFASSEEHSFYGRLTVWGNLR
                     FYAQLHALSPARWKARYDELKNVLDLPAPETPFRQLSAGQKQKLLLARAVLHDPPVLI
                     LDEPSQHLDPAFLARFRTLATEEWGERRKKIVLISTHHLEDALKISDRWLVISEGRLR
                     FDGSVSAVKRADPAFAVDSFFQKLTAPEEGGPRAD"
     gene            1745..2554
                     /locus_tag="P5079_09990"
     CDS             1745..2554
                     /locus_tag="P5079_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY30350.1"
                     /translation="MPTRILWAFFRRDFTIHTSYRLGFAIDLLGVFFSAATFYFVAKM
                     VGPSAMPLLKNYGGDYFAFVLIGIAFSTYQNVGLNSFAQSLRQEQFINTLEPLLLTPV
                     TLPQFLLGSSLWDFLYATAQVGFYLALGMVVFGLRLAEANVGPAGAVLVLTLIAFMGL
                     GVLAAAFIMRFKRGNPVTWLVVTASELLGGVYFPVEILPGWLKSLSRFVPMTHALTGL
                     RKCLLMGAGWGEVLPQLTALALFALAAWPLGLWGFSAALKRARADGSLGHY"
     gene            complement(2548..2949)
                     /locus_tag="P5079_09995"
     CDS             complement(2548..2949)
                     /locus_tag="P5079_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30351.1"
                     /translation="MEKKIHLLIVDDDAALTHFCRKVVEKYACRATVASGVEEAVRTL
                     SADTSIDFVLSDVQMPGVDGWTLLSWMKQNRPGCPVGLMTGIPYVSVRETAVKAGAVG
                     VLMKPFSPDDLCRFVRSHLGVSAGFERKSVQ"
     gene            3050..3412
                     /locus_tag="P5079_10000"
     CDS             3050..3412
                     /locus_tag="P5079_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY30352.1"
                     /translation="MDSIHIRLGRKLRAERERQGLTQEIVAERCNLSTAFIGQIERGQ
                     NAASLTSVERIGRALNVSLSELFSFPETRPSKPSLSSYEKICSLLRGRPKREQEYILA
                     TVKTLVRRLRQAGKGKRR"
     gene            3654..4061
                     /locus_tag="P5079_10005"
     CDS             3654..4061
                     /locus_tag="P5079_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY30353.1"
                     /translation="MKFNRTLAYGLSCLQHLSEHADQGWIETHHIASAQGLPESYCNK
                     VLQSLVHARLVLSSRGRGFKLAKPLNDISAWEVMEAFTFNGAPPPDRKNASDKLYESL
                     RERVNRMLAGLTLDEVVASVQSQEERLEPKSDG"
BASE COUNT          791 a         1326 c         1269 g          853 t
ORIGIN      
        1 cctcctgaag ggaccgacct tcgccgagac gatgaaggtc ttccgcgacc cccgtctgcg
       61 ggtgagcgtg cagggagacc ggccggagga ccacgacacc cccacgcctc tctgcgaagc
      121 cggccgttcc tacgtggccc tggggccgaa cggcgacgtg tatccctgcc tccaatggcg
      181 ggcggtgttg ggcaacgtgc ggcggaacgc gctgaaagac atctggaact cggaggaagc
      241 ccgccgacga cgggcgatga cccgggcgga ttacgccgtc tgcaacgcct gcgatcttct
      301 ggcgtactgt ccccgatgca ccggcgtggc ggaacgggaa gacggggatt tcctcggccc
      361 ctcggccgcc gcctgccggc tggcccgcct gaaccgggac gtgctcgtcg gccgttcccg
      421 aagcggggcg gaaagaccgg cggcggaagc ccacccttta taaatccctt caggagaacc
      481 tcatgtcaaa aaagaaaaaa ccgtataaaa aaccgacgct gttatcggaa aagatcatcg
      541 agcaggccgc tctcggctgc aaaaaatgca ccagcggcaa ccccatcgta caggcccgct
      601 gcagcgccca gctctcctcg tcttgacccg cgggtcccgt tccgacatct tctgggtcga
      661 aacgcgcggc ggctgcatgc ggccgttcct ctcctcgcgc agccgcctcg tgcccctgct
      721 caaaaccgcc gtctgccgtg cgggagacgt ggcgctctgc ggggagccgg accgcgccgt
      781 cctgcaccgc ctttaccgcc ggcaaggaaa cggatggtgg gcggccgacg acgccgcccg
      841 gacgccgttc caatgggtgc ccgacggcgc gttgcggggg cggctgctcg gccacacgcc
      901 gcaggggacg cccggcctgc tctggggata cgcggcgcgg atcctgttct ccgccgcccg
      961 gagattcaaa cgacttttca acgtttcctc ctgatggacc tcgtcgtctc gcacgccttt
     1021 aaacagttcc cttcctccgc cggcccgaaa aaaatattga aggacatctc ctttcgtgtc
     1081 acggccgggg aagtggtctg tctgctgggt ccgaacgggt cgggaaaaac aacccttctg
     1141 aagaccatcg ccacccttct gtcgccggac ggcggggagg tccttctcga cgggaagagc
     1201 gtgcactcgg acaccctccg gacccgacgg ctcctcgggt tcgcctcgtc cgaagaacat
     1261 tccttttacg gccggctgac ggtgtggggg aacctgcggt tttatgcgca actccacgcg
     1321 ctttctccgg cgcgctggaa ggcgcgctac gacgaactaa agaacgtcct ggacctgccg
     1381 gccccggaaa cgccgttccg ccagctttcc gccggacaaa aacaaaaact cctcctggcg
     1441 cgggcggtgc tgcacgaccc gccggtgctg atcctggacg agccgtcgca gcatctcgat
     1501 cccgcttttc tggctcggtt ccggaccctg gcgaccgagg agtgggggga acggcgaaag
     1561 aagatcgtgt tgatctccac gcaccacctg gaggacgcgt tgaagatctc cgaccggtgg
     1621 ctggtgatct cggaaggccg gctccgtttc gacggttcgg tgtcggcggt gaagcgcgcc
     1681 gacccggcgt tcgccgttga ctccttcttc cagaagctga cggccccgga ggagggaggc
     1741 ccgcgtgccg actaggatcc tctgggcgtt cttccgccgg gacttcacca tccacacctc
     1801 ctaccggctg ggcttcgcca tcgatctgct cggcgttttc ttctccgccg ccacgttcta
     1861 tttcgtggcg aagatggtgg gtccgtcggc gatgccgctg ctgaagaatt acggggggga
     1921 ttatttcgcc ttcgtgctga tcggcatcgc gttctccacc taccagaacg tgggcctgaa
     1981 ttccttcgcc caatcgctcc gccaagaaca gttcatcaac acgctggaac cgttgctgct
     2041 gacgccggtg accctgccgc agttccttct gggctcctcg ttgtgggact tcctgtacgc
     2101 cacggcgcag gtggggttct acctggccct ggggatggtc gtcttcggtc tgcggctggc
     2161 ggaggcgaac gtggggcccg cgggggcggt gctggttttg acgctgatcg ctttcatggg
     2221 gctgggggtt ctggcggcgg cgttcatcat gcggttcaag cgggggaacc ccgtgacgtg
     2281 gctggtggtg acggccagtg aacttctggg cggggtgtat tttccggtgg agatcctgcc
     2341 gggatggttg aaatcgctct cccgttttgt ccccatgacg cacgcgctga cgggactgag
     2401 gaaatgtctg ttgatgggtg ccggatgggg ggaggtgctg ccgcagttga cggccctggc
     2461 gctgttcgcg ttggcggcgt ggccgttggg cctatgggga ttttccgcgg cgttgaagcg
     2521 ggctcgtgcg gacgggagcc tgggccacta ctgaaccgat ttccgttcga aaccggcgga
     2581 gacgccgaga tggctccgga cgaagcggca gagatcgtcg ggcgaaaaag gtttcatgag
     2641 gacgccgacg gcgccggctt tgacggcggt ctccctcacc gaaacgtagg ggatgccggt
     2701 catcaggccg accggacatc ccggccggtt ctgcttcatc caactcaaga gggtccagcc
     2761 gtcgaccccg ggcatctgca cgtcgctcag gacgaaatcg atgctcgtat ccgccgacaa
     2821 cgtccgcacc gcctcttcca ccccggacgc cacggtggcg cggcaggcgt atttttccac
     2881 caccttccgg caaaaatggg tcaacgccgc gtcgtcgtcc acgatgagga gatggatctt
     2941 tttttccatg agaggagtat atgtcatttg atacttatag gcaacgttcc caaaaatatc
     3001 tacaatcgct tacattttcc ctttctaacg tttatactct atttatctta tggattccat
     3061 tcacatccgt ctgggaagga aactccgggc ggagcgggaa cgtcaagggc tcactcaaga
     3121 aatcgtggcg gaacggtgca acctctccac cgccttcatt gggcagattg aacgtgggca
     3181 aaacgccgcc agtctcacca gcgtggaacg gatcgggcgg gcgctgaacg tctccctctc
     3241 cgaacttttt tcttttccgg aaacccgtcc ctcaaaacca tccctctcct cctatgaaaa
     3301 aatctgttcg ctgctgcgtg ggcgtccgaa acgcgagcag gaatacattc tcgccacggt
     3361 gaaaacgctc gtccgtcggt tgcgccaggc gggcaaaggc aaacgccgct agttccgacc
     3421 ctgtttcccg ccgcacagat tcagtcaatc tcgtcaaaag aatcttccat cggatacttt
     3481 tactccccag ccatcggata aaaaatatct catatgtgac aaaagtcact attgaatttg
     3541 tttcaaaggc catatacact ttaggtatac aacttataca gcttttgcct ttttcggaac
     3601 attttcatga ctgcttcgtt tcaatagtaa caatctgaaa ccagagaggc cctatgaaat
     3661 tcaaccgcac gctcgcctac ggattgtcgt gtctgcagca tttgtcggag catgccgacc
     3721 aggggtggat cgaaacccac cacatcgcca gcgcccaagg gttgccggaa tcctactgca
     3781 acaaagttct gcagagtctg gtgcacgcgc ggctggtgct ttcttcccgc ggccggggat
     3841 tcaaactggc caaacccctg aacgacatca gcgcctggga agtgatggag gcgttcacct
     3901 tcaacggcgc gccgccgccg gaccgaaaaa acgcctccga caaactttat gaaagccttc
     3961 gggagcgcgt gaaccgcatg ttggcgggac ttactttgga tgaagtggtg gcttcggtgc
     4021 aatcgcagga ggaacgccta gaacctaagt cagacggctg aaccgcccga ggaaaaatgc
     4081 ccctacgccg agaccgacga aagccgatcc cctcttcccg ccgagccccc cgaagggccg
     4141 gcggtctcgg tcttttattg gcgctccttt ttctgtccgc cggtcaaaaa ctttcggcgg
     4201 ccacgcggat caagaccgtg gagtacaatt tcgggtccc
//
LOCUS       DAOTFD010000215         4235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15353_length_4235_cov_4.246890, whole genome shotgun sequence.
ACCESSION   DAOTFD010000215 DAOTFD010000000
VERSION     DAOTFD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15353_length_4235_cov_4.246890"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4235)
                     /locus_tag="P5079_10010"
     CDS             complement(<1..>4235)
                     /locus_tag="P5079_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY30354.1"
                     /translation="QSFEEAHQHALDAQGKDATAEVVSALKDYLNAPTKSWVDLNAPL
                     LKDYTQVSVQFEQGPDRGLLDEVNRRYGEPTHPDKRPWITRYIAQMFMTGGLGALMHD
                     LVVAWKKNGVDSISVNPLYNNNIKGVVDTSKLRLKPGQVLGDVFRDVLGKPIMSFSFP
                     LGDDDAFRQGAQASKSRAIIGKEIHVNVHRRFTKVGGTPLYYLDAYYLDDGGNQVRVF
                     DELYPDNDYRGVQMAIYNKASQRMLLELQKKGAVKKKIIFTENEVFVSLPKNLFPDAI
                     RHHINHTVWKPGMYRPPTYFYGLMDFPEELRDLITRDGKIAIEEFAAITVDVLSGVGL
                     YEHTPVLRKEVFPLYTHKIVGYNQGGLRSTNGALLDQWQGPEIRGLIDAYKKLLGMDP
                     LVDDIQFYAALARNSVLSEEFSARFEALHALYALDLLIWLHDYQDQPLGGSTWLTDLL
                     KGTSLDPAALRALRDEYRTGIEQALKDVGRWNAVLSELNAARPELSPLLEKVLRHPMA
                     ANVRRQVAYKGPDKYEELFQCFILADILRTNSRAIEQFEKLLAEDTEWLRADWNAIDP
                     RLVALWKDLLNIQEGDSDEVIKDKVQRHNWDDTKAKLYVRLATVKENPNMLNEFRESG
                     TRLILGGRTFGSDARHLFDLLKGMAKTLNLDNQVAFIENYNIEDAPTIFRGVSASVMM
                     SDEFLEASATSMMKAVTNGAALIGVWGGAEPELFTIIETATGKEVDVFSPGVTHDLIA
                     AGLKDGTYKIVNGFLVEYMRPEKLTGKAKELAMSREEMGGRRPDAQSLLQSFVTLGRM
                     AANPVERRNLAYQTLASSPKVDIERSQARAHIKMWEAAIKARKAYDAFVQAMPVKVEE
                     VSGALNLRGGGFGWKYKENGVTEVFLPIACEPGLVGFLKSFRRLRALGDRAVWSLMHH
                     ASPAYPQKVSGGDVFAYLDSLLEQTPTLKPLRDKLQEFGKAVAAADSPQAKVQITLAA
                     LDFVEQVVRRLQSPRAGLTRAPPAEAPWYRKNLLQIRVRSYGARKEGDTVVPGHLKNI
                     RADLKKFKKDGFSTLYLLGLIAPSELSRKINRGEHDDPAQGLYISRDPATGQKVTVCR
                     NMWGGDKTREGSLFSISDPKALNPQLGTLDDLKQLIDEAQPLGLKVIVDFVPNHMSVD
                     SPLIREHLDWFIHRRLTPAEEKLPDEELLSRVPNYFIHYAKNGRRVLVAHGKDPNFDG
                     WTDTAQLDYSNPELRAYMISVIKHWASLGVNGIRADMAMMILSNQIRGQWNRSMPEGY
                     EFWREAVQSVKADYPDFFFLAETYWAKEGELLGLGIDATYDKYFLDILRRGDVEEIRN
                     YLSRVPLDYLQRLAHFLENHDEQRAASTLGLPDGSLGKLRAALLLLVTAPGMPFIHDG
                     QMDARQIFSPAQQVVIPDEKPNEKFRRYLSNML"
BASE COUNT          776 a         1195 c         1321 g          943 t
ORIGIN      
        1 ttcaacatgt tggataaata ccgtcggaat ttttcgtttg gtttttcgtc agggatcacc
       61 acttgctggg cgggactgaa aatctgtcga gcgtccatct ggccgtcatg gatgaacggc
      121 attccggggg cggtgaccaa caggaggaga gcggctctca gtttgccgag ggatccgtcc
      181 ggtaaaccca gcgttgaggc cgcccgttgc tcatcgtggt tttccaggaa atgggccagt
      241 cgctgcaggt agtcgagcgg gacgcggctc agatagttac ggatttcctc cacgtcgccc
      301 cggcgcagga tgtcgaggaa atatttgtcg taggtggcgt cgatgcccag ccccagaagt
      361 tccccctcct tggcccagta ggtttctgca aggaaaaaga aatccggata atcggctttg
      421 accgattgga cggcttcccg ccagaactca tacccttccg gcatcgaccg gttccactgg
      481 ccgcgaatct ggttgctcag gatcatcatg gccatatcgg cgcggatgcc gtttactccc
      541 aaggacgccc aatgtttgat gacggaaatc atgtaggcgc gcaattcggg gttggaatag
      601 tcaagctggg cggtgtctgt ccagccatcg aagttggggt ctttcccgtg cgccaccagc
      661 acgcgccggc cgttcttcgc gtaatggatg aaataattcg gaacacgcga aaggagttct
      721 tcgtcgggaa gtttttcctc ggccggtgtc aaacgccggt ggatgaacca gtcgaggtgt
      781 tcccggatga gcggagaatc cacggacata tggttcggca caaagtccac gatgaccttg
      841 agccccagcg gctgggcttc gtcgatcaac tgtttcaggt catccagagt gccgagctgg
      901 gggttgaggg ctttcggatc cgagatggag aagagcgatc cttcccgggt tttgtcgccg
      961 ccccacatgt tacggcagac ggtcactttt tggccggtgg ccggatcccg ggagatgtaa
     1021 agcccctggg ccgggtcatc atgctcgccg cgattgattt ttcgggaaag ctcgctgggc
     1081 gctatgaggc cgaggagata aagcgttgaa aaaccgtctt ttttgaattt cttgaggtcg
     1141 gcgcggatat ttttcaaatg accgggaacg acggtgtccc cctccttccg cgccccatag
     1201 gaacgtacac ggatttgcag gaggtttttc cggtaccacg gagcctcggc tggcggcgcc
     1261 cgggtgagtc ccgcccgcgg cgactgaaga cggcgcacca cctgttcgac gaaatccaaa
     1321 gcggccagcg tgatttgcac cttggcttgc gggctgtccg ccgcggccac cgctttgcca
     1381 aattcctgca gtttgtcgcg caacggtttt aatgtgggcg tctgttcgag cagggaatcc
     1441 aggtaggcga acacatcgcc gcctgacact ttctggggat aggcggggga ggcatggtgc
     1501 atcaaactcc agacggcccg atcccccaag gcacgcaaac gacggaaact tttgaggaaa
     1561 cccaccagac cgggttcaca cgcgatcggc aggaaaacct cagtgacgcc gttctcttta
     1621 tatttccagc cgaaccctcc cccccggagg ttcagcgcac ccgagacttc ttctactttg
     1681 acgggcatcg cctgaacaaa agcgtcgtag gcttttcggg ctttgatggc ggcttcccac
     1741 atcttgatgt gcgcgcgggc ctggctgcgc tcgatgtcga ccttggggga actggccagc
     1801 gtctgatagg cgaggttccg ccgctccacg ggattggcgg ccatgcggcc gagcgtaacg
     1861 aacgattgga gcaggctttg ggcgtccggc cggcggccgc ccatctcttc ccggctcatg
     1921 gccagttctt tggccttgcc ggtcagtttt tccggtcgca tgtattcgac gaggaatccg
     1981 ttgacgatct tgtaggtgcc gtccttcagc cccgccgcga tcaggtcgtg ggtgacgccg
     2041 ggagagaaca cgtccacctc tttaccggtg gcggtttcga tgatggtgaa gagttccggt
     2101 tccgccccgc cccacacgcc gatgagggcg gcgccgttgg tgacggcttt catcatgctg
     2161 gtggcggaag cttccaggaa ttcgtccgac atcatgaccg aggcggaaac gccgcggaaa
     2221 atggtggggg cgtcctcgat gttgtagttc tcgatgaaag ccacctggtt gtccaggttc
     2281 aaggttttgg ccatcccctt gagcaggtcg aaaagatggc gcgcgtccga gccgaaggtc
     2341 cgcccgccga ggatcagccg ggtgccggac tcgcggaact cgttcagcat gttcggattt
     2401 tcttttaccg tggccagccg gacgtaaagc ttggctttgg tgtcgtccca gttgtgtctc
     2461 tggaccttgt ccttaatgac ctcgtcgctg tccccctcct ggatgttcag aaggtctttc
     2521 cacaacgcca ccaggcgcgg atcgatggcg ttccagtccg cccggagcca ttcggtgtct
     2581 tccgccagca atttttcgaa ttgttcgatc gcccgcgagt tcgtccggag gatgtccgcc
     2641 aggatgaaac attggaaaag ttcctcgtac ttgtccggcc ccttgtacgc cacctgccga
     2701 cgcacgttgg ccgccatggg gtgccgtagc actttttcca ggaggggcga aagttcgggc
     2761 cgcgccgcgt tcagttccga cagaacggcg ttccaccggc cgacgtcttt cagcgcctgc
     2821 tcgatccccg tccgatattc atcccgcaac gcccggaggg cggcggggtc cagagatgtc
     2881 cctttcagaa ggtcggtcag ccaggtggat ccgcccaagg gctggtcctg ataatcgtgc
     2941 agccagatga gaaggtccag ggcgtagagg gcatggagag cttcaaagcg ggcgctgaat
     3001 tcttcggaaa ggacggagtt tcgggccaaa gcggcataaa actggatgtc gtccaccagc
     3061 ggatccatgc ccaacagttt cttgtaggca tcgatcaacc ctcgtatctc cggcccttgc
     3121 cactggtcga gcagagcgcc gttggtgctg cgcagtccgc cttgattgta accgacgatc
     3181 ttgtgggtgt agagcgggaa gacctctttc cgcaacaccg gggtgtgctc gtacaggcct
     3241 acgccggaaa gcacgtccac ggtgatggcg gcgaattcct cgatggcgat cttgccgtct
     3301 cgcgtgatga ggtcgcgcaa ctcctccggg aaatccatca ggccgtagaa atacgtcggc
     3361 ggccggtaca tgcccggctt ccagacggtg tggttgatgt gatggcggat ggcgtccggg
     3421 aacaggttct tcggcaggct tacgaagact tcattctccg tgaagatgat cttctttttg
     3481 acggcgcctt tcttctggag ttccaaaagc atccgctgcg aggccttgtt gtaaatggcc
     3541 atctgcacgc cgcggtagtc gttgtccggg taaagctcgt cgaagacgcg aacctggttg
     3601 ccgccgtcgt cgaggtagta ggcgtccaga taatacaacg gcgtgccgcc taccttggtg
     3661 aaccggcggt gcacgtttac gtggatttct ttcccgatga tggcgcggga cttggacgcc
     3721 tgggcccctt gacggaaagc gtcgtcgtcg cccagcggga aggagaacga catgatgggt
     3781 ttgccgagga cgtcgcggaa cacgtcgccg agcacctggc cgggcttcag gcggagtttg
     3841 ctggtgtcca ccacgccctt gatattgttg ttatacaacg ggttgacgga gatgctgtcc
     3901 acgccgttct ttttccaggc gacgacgaga tcgtgcatca gggcgccgag cccgccggtc
     3961 atgaacattt gggcgatgta gcgggtgatc cagggccgtt tgtcgggatg ggtgggttcg
     4021 ccgtaccggc ggttgacctc atccaagaga ccgcgatccg gcccctgttc aaattggacg
     4081 ctgacttggg tgtaatcctt taagagcggg gcgttcaggt ccacccagct cttcgtgggg
     4141 gcattcagat aatctttgag ggcggaaacg acttcggcgg tggcgtcctt cccctgggcg
     4201 tcaagggcat gctggtgggc ttcttcaaag gattg
//
LOCUS       DAOTFD010000216         4193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15558_length_4193_cov_3.839053, whole genome shotgun sequence.
ACCESSION   DAOTFD010000216 DAOTFD010000000
VERSION     DAOTFD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15558_length_4193_cov_3.839053"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="P5079_10015"
     CDS             <1..548
                     /locus_tag="P5079_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30355.1"
                     /translation="KRSLAIDPDDIVAITGLADIYSQHLDDLPAARKLLKRAKLPPDA
                     DTGTIYAELMSPRWLKQKEAAAFLKKNLLAIHDACRRKNIRLVLQTYPYPRGIEQTLA
                     SVAELCRIPLIDHARRFREIGAYGTPLDYFIEDNGDWRISHLNDAGYRQMALAVADDL
                     EKHELRPSPLLALPSHNDHSP"
     gene            765..1790
                     /locus_tag="P5079_10020"
     CDS             765..1790
                     /locus_tag="P5079_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRY30356.1"
                     /translation="MASARSSGYAAPKTSASQEIEADISGVSGSVGKTSERGFRLNST
                     AGSPFVGTKLLSSSGFEIFSGLWGGGAAFRSAGETTLVTLGDPDHPATLTIPPGALSS
                     DFVVFAATSPLTRPLYAAPATILEANENLARSFTAYTQPLDGCLWEFLVLDADGNRHT
                     APLQAPATLSLPYRDANNDGLVDDTHPPLRTQNLSIWWLDETHRLWVKLSPATLDTAH
                     RRVSAPIRHFSVFTVMGTPSFHVGDAFAFPVPWRPEGPQAGAGANQTGTLSDGITFSN
                     LPSEAKIRIHTLSGEIVREIDHNDGLPQKKWDAKNSDGEDAVSGTYIYVIDAGGQKKF
                     GKIVIIR"
     gene            1787..2965
                     /locus_tag="P5079_10025"
     CDS             1787..2965
                     /locus_tag="P5079_10025"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30357.1"
                     /translation="MSPSPLLSRPLAVISLLSFALLFSPPTGLAENYGTTSGEILKLP
                     VGARAVGMGEAQTALPHDLSASHWNPAGLGTLSSPEISLMHHRLYEDVQQYYALVACP
                     LGALGTPAVGFNHLTVESFQGFDAQGTPTRPLESRADAASLSWGKNLFSGASDPSGLF
                     GGLTFKFLQEEHADVSASGLATDVGFLYRPPLPAAPAWGWVRRTSLGLAVKNLGQGPK
                     FDTQTPPLPTEITAGVAYSHFLEGDILHLALDVRKNAEAGNGGAFGVEYGTRDLLALR
                     AGFVTTRDRSAGLRLGAGFRLRRVRLDYAWSANGDDLDASHRLSLTFQWGTPSSRQDP
                     VLLRDVFNDHLAQGKKWMELGAYDRAVLQFQQALEARPGDPEAHKLLLDCGKRMETLP
                     "
     gene            3313..>4193
                     /locus_tag="P5079_10030"
     CDS             3313..>4193
                     /locus_tag="P5079_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30358.1"
                     /translation="MDEARRHLERTKTREALLTARCREARTRLIEGEILQASDIYNQV
                     LETAPGYPPAVKGITHVQKHLRAALRRGEFASPQHQAAAEGTLEYNSARWDNAAPLLE
                     SALGSAILAEDLTEAHVGEYAAKAKDRQKKELWRVRRAALLDDALREYRAGRLKTAQI
                     GLKTLIEIDPSDAEAKSHWALVEKMLEEARAVAQEETRRAEIEKILAEADLWAQQSLF
                     DDAVEALSRALELDPSHPEIRRKLDSLRETMKNEGRPLPPIAPEDDAEELYRKGLQSF
                     GNGNREEALKTFQEVLRR"
BASE COUNT          858 a         1472 c         1139 g          724 t
ORIGIN      
        1 tcaaacgaag ccttgcgatc gaccccgacg acatcgtcgc catcaccggg ctggccgaca
       61 tctattccca acacttggac gacctccccg ccgcccggaa attattgaaa cgcgcgaagc
      121 tgcctccgga cgccgacacg gggaccattt acgccgagtt gatgtcgccc cgctggctca
      181 aacagaaaga agccgccgcc ttcctgaaga aaaacctttt ggccatccac gacgcctgcc
      241 gccgaaaaaa tattcgactc gtccttcaga cctaccctta ccctcgcggc atcgaacaaa
      301 ccctggcctc cgtcgccgaa ctctgccgaa tccccctcat cgaccacgcc cgccgcttcc
      361 gggaaatcgg tgcctacggg acaccgctcg actatttcat cgaggacaac ggcgattggc
      421 ggatcagcca cctgaacgat gccggttacc gccaaatggc cctggccgtg gccgacgacc
      481 tggaaaaaca tgaattacgg ccttctcccc tcctcgcatt gccttcccac aacgaccatt
      541 cgccgtaacc cctcgaccgt tccacacctt ccaagctccc gacaaaatcc agtcaatccg
      601 gtcatctcgg gataagactt cccatccttc cgcggatttg gtattaactt tccttgtatt
      661 cgcgggcgag ggaacggcag ggaagtccat ggggacggac cataccactc atcccggagc
      721 ccccgacgaa acgaacacct ctcctgtttg cggccatcgt tctcttggcg tccgcccgct
      781 cctccggtta tgccgccccc aaaacttccg cctcccagga aatcgaggcg gacatctccg
      841 gcgtctccgg ttccgtcggg aagacgtccg aacgagggtt ccgtctcaac agcaccgccg
      901 ggtccccctt tgtcgggacg aaactcctct cctcctccgg tttcgaaatc ttctccggtc
      961 tgtggggcgg aggcgcggcg ttccgctccg ccggagaaac cacgctcgtc acgctcggcg
     1021 atccggacca ccccgccaca ctgaccatcc ctccgggcgc cctctcctcc gatttcgttg
     1081 tctttgccgc cacgtctccc ctcacccgac cgctttacgc cgcgcccgcc accatcctgg
     1141 aagccaatga aaacctcgcg agaagtttca ccgcctacac gcaaccgctc gacggctgcc
     1201 tttgggaatt cctggtcctg gatgccgacg gaaaccgcca caccgccccg cttcaggccc
     1261 ccgccaccct cagcctgccc taccgggacg cgaacaacga cgggctcgtg gacgacacgc
     1321 atccgccgtt gcggacgcaa aacctgtcga tctggtggct ggacgaaacc catcgtctct
     1381 gggtgaaact ttcccccgcc accctggaca cggcccaccg gagggtgagc gcacccatcc
     1441 ggcatttttc cgtcttcacc gtgatgggga ccccttcctt ccacgtcggc gacgcctttg
     1501 cttttcctgt cccctggcgg ccggagggtc cccaggccgg cgcgggggcg aaccaaaccg
     1561 ggacactgtc cgacggcatc acgttctcga acctccccag cgaagccaag atccgcatcc
     1621 acaccttatc cggagaaatc gtccgggaga tcgaccacaa cgacggcctg ccccagaaaa
     1681 aatgggacgc gaaaaacagc gacggagaag acgccgtcag cggcacgtac atctatgtga
     1741 tcgacgccgg cggccaaaag aagttcggaa aaatcgtgat catccgatga gcccctcccc
     1801 tcttctctcc cggccgctcg cggtcatttc gctactttcc ttcgccctcc ttttttcccc
     1861 gccgaccggc ctggccgaaa attacgggac cacgagcggc gaaatactga aactcccggt
     1921 cggggcgcgg gccgtgggga tgggagaggc ccaaaccgcc ctgccccacg acttgtccgc
     1981 ttcccactgg aaccccgccg ggctggggac cctctcctcc ccggaaattt cactcatgca
     2041 tcaccgtctt tacgaggatg tccagcagta ttatgcgctc gtggcctgtc ccctgggcgc
     2101 gctcggcacc ccggccgtcg gcttcaacca tctgacggtg gaatccttcc aggggtttga
     2161 cgcccaaggc acgcccacgc ggccgctgga gagccgcgcc gacgccgcct ctttgagttg
     2221 gggcaaaaat cttttttccg gggcctccga ccccagcggt ctcttcggcg ggctgacgtt
     2281 caaatttctg caggaagaac acgccgacgt ctccgcttcg gggttggcga cggacgtggg
     2341 gtttctttac cgtcccccgc tgcccgccgc cccggcctgg ggatgggttc gacggaccag
     2401 cctgggtttg gccgtcaaaa atctcggaca agggccgaaa ttcgacacgc aaacgcctcc
     2461 ccttccaacg gaaataaccg cgggggtcgc ctattctcat ttcctcgaag gcgacatcct
     2521 ccatctggcc ctggacgtcc ggaaaaacgc ggaggcggga aacggcggag ccttcggggt
     2581 tgaatatggg acccgggacc tcctcgctct ccgcgccgga ttcgtgacga cacgggaccg
     2641 ctccgcggga ctgcgcctgg gcgccgggtt ccgactgcga cgtgtccgac tcgactacgc
     2701 ctggtccgcc aacggtgacg atttggacgc ctctcatcgg ctcagtctca ctttccaatg
     2761 gggaacaccc tcttcgcgcc aggaccccgt cctcctccgg gacgttttca acgaccacct
     2821 ggcgcaaggg aaaaaatgga tggaactcgg cgcttacgac cgggcggtcc ttcagttcca
     2881 acaggccctg gaagcacgcc ccggcgatcc ggaagcccat aaactcctcc tcgactgcgg
     2941 caagcggatg gaaactcttc catgacggcc ctttcaaaac gctctcccct gtccctcggt
     3001 gtcgccctcc tcttcgcgtt tgtgccgccg ggagtgaaaa gcggccctct tccatcgcca
     3061 gcgccgctcc ccacggcatc gacggacgca tcgcccgccg ggcccgaaca aactcccgga
     3121 gacgtccgcg ctcttttgaa ttccgcccga aatgcggaga aagccggaag gcccctggaa
     3181 tcgctcgaac agtatctgac agcctttgcc ctcgacccga gcaacgccca agcgaacgcc
     3241 ggcctccgcc ggattgctaa acggatccga aaaagttgga gtcaaaaaat aaaaaagatt
     3301 gaacacgtct acgtggacga agcccggcgg catctggaaa ggacaaaaac gcgggaagct
     3361 ctcctgaccg cgcggtgtcg tgaggcgagg acccgcctga tcgaaggaga aattttacaa
     3421 gcgtccgaca tatacaatca ggtcctcgaa acggcgccag gatatccgcc ggccgttaag
     3481 ggcatcaccc acgtccaaaa acaccttcgc gccgcgctcc gaagaggcga gttcgcttcc
     3541 ccccaacatc aagccgcggc ggaaggaacc ctcgaataca attcagcccg atgggacaac
     3601 gccgcccccc tgctggagag cgcgctgggc tcggcgattc tggccgaaga tttgacggag
     3661 gcccatgtgg gagagtatgc cgccaaagcg aaagaccggc aaaagaagga actctggcgg
     3721 gtacgccgcg ccgcgctgct cgacgacgcc cttcgggaat accgcgcggg ccggctcaaa
     3781 acagcccaaa tcggactcaa gacgttgatt gagatagacc cgagcgacgc ggaggccaaa
     3841 tcccactggg cgctggtcga gaaaatgctg gaggaggcga gagcggtcgc ccaagaagag
     3901 acccggcgcg ccgagatcga gaaaatactc gccgaggcgg atctctgggc ccagcagagc
     3961 ctttttgacg acgccgtcga agcgctgtcg cgcgctctgg aattggaccc ctcccatccg
     4021 gaaatccggc ggaaactgga ttctctccgg gaaacgatga aaaacgaagg tcggccttta
     4081 ccgcccatcg ccccggaaga cgacgccgaa gaactctacc gcaaaggttt gcaatcgttc
     4141 gggaacggga accgggagga agcgctcaaa acgtttcaag aggtccttcg ccg
//
LOCUS       DAOTFD010000217         4181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15625_length_4181_cov_3.839069, whole genome shotgun sequence.
ACCESSION   DAOTFD010000217 DAOTFD010000000
VERSION     DAOTFD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15625_length_4181_cov_3.839069"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="P5079_10035"
     CDS             <1..647
                     /locus_tag="P5079_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30359.1"
                     /translation="RLGQGKYQEALTHYAEALKIFPFFPEIHNNIGITFLSLDRPAAA
                     AKHFRLALLRKPRHAVIRTNLGYALSREGKIAEALHEYRTALQSDPENVQAHYNLSCA
                     LMDLGRLEEALGEAQETVRLSPSFAKAHYTLANLYVKLSRLPEAVERYRETLRHDPRH
                     TDARFNLAGLLYLSGRPLEAAAEYQTLLKINPNDRDARTKLRLILDQNHGNPAR"
     gene            787..2427
                     /locus_tag="P5079_10040"
     CDS             787..2427
                     /locus_tag="P5079_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30360.1"
                     /translation="MNNPHYRGLGWIHLRWMWTSFHMGHYMPVTWMSLGLDHVLWGMN
                     PAGYHLTSLLLHAVNAGLFYFLALRLLGPADGGEPPGAAPLRFFAAGFSALFFSLHPL
                     RVESVAWVTERRDVVSGLFCLVSLLFYLRYVASPSGGRARFVNYIASFVSYAVCLLSR
                     ANLFVLPLLLLVLDVYPLRRFSKGVAGRDRLFWEKVPFALIAAAAVVWGLEGLLQVNS
                     SVSFARHGIFQRLALASFSAVFYVGKTVFPLRLSPLYEIPLRFSPWEARFVVSGAVVV
                     LLSWQLWRRRRRWPAGVAAWISYLLLIAPTSGLFQRGIQIAADRYSYASCGGWALLAG
                     AALRWAMQKFPSVAWRRGVLAGAAAVSAVLGASTWAQTHVWRDSETLWRHVLRVQPHS
                     AVAHYNLGVILSEQHREDEALRHWEETLRYDPGDADAYVQVGILYEERGRLSEAAELY
                     KTALRWKPESVEAHFNLGILLGKEGNLKGAEMHLHRALLLAPRTAVVWHSLGRVLERQ
                     NKFDFAAECFRRALSLEPARADSRDALNDLLEKHPAKK"
     gene            2580..3587
                     /gene="galT"
                     /locus_tag="P5079_10045"
     CDS             2580..3587
                     /gene="galT"
                     /locus_tag="P5079_10045"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRY30361.1"
                     /translation="MPELRRDPLIGRWVIISTERKRRPSDFMHARESQGSVPCPFCPG
                     NEEKTPPEISAYSSPGRLPNRPGWWVRVVPNKYPALRVEGQIHRTGDHLYDKMDGLGA
                     HEVIIETEDHGKQLEDLSDERLQDVFWTYRDRINDLKKDPRLEYILIFKNRGVAAGAS
                     LAHAHSQLIATPMVPIRVKQEMRGADDYYERRGRCIFCDMVQQELAQKVRVVDENPGF
                     VAITPFASRFPFEISVLPKKHRSDFSEIPKSEVIELGRMIKSVFQRLNRVLDNPSYNF
                     VLHDSPLKDSHLEYYHWHIEIMPKLSQTAGFEWGTGFYINPTPPEQAAEYLKEIETIA
                     G"
     gene            3613..>4181
                     /locus_tag="P5079_10050"
     CDS             3613..>4181
                     /locus_tag="P5079_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRY30362.1"
                     /translation="MKILLAASEAVPFCKTGGLGDVVGSLLSALRGLRHDARLVLPKH
                     RAINEKRFGLRRLPYRLTLPLGDGDETAALWEARLHRTAPVYFVDAPKYFDRAGLYVD
                     ETGRDFADNDDRFILFSRAVLETAKAVDFRPDVLHAHDWQTGLAPAYLSTVYRHDAFF
                     IPTAACFTVHNMAYQGLFPKDALFRAGFS"
BASE COUNT          721 a         1306 c         1230 g          924 t
ORIGIN      
        1 tccggctggg acagggaaaa tatcaagagg cgctcaccca ctacgcggaa gcgttgaaga
       61 ttttcccgtt cttcccggaa atacataaca acatcggcat tacgttcctg tcgttggacc
      121 gccccgcggc ggcggcgaaa cattttcgct tggcgcttct acgtaaaccc cgtcatgccg
      181 tcatccggac aaatttaggg tatgcccttt cgcgggaggg gaagatcgcg gaggccctcc
      241 atgaatatcg gaccgccctc cagtccgatc cggaaaatgt ccaggcccat tacaacctga
      301 gttgcgcact gatggatctg ggtcggctcg aggaggcgct cggtgaggct caggagaccg
      361 ttcggttgag cccgtctttt gcgaaggccc attacacgtt ggcgaacctc tacgtcaaac
      421 tttcccgcct gccggaggcc gtcgagaggt atcgggagac cctgcggcat gacccgcgcc
      481 atacggacgc ccgtttcaat ctggcgggac ttctttatct gtccggccgg ccgctggaag
      541 ccgccgcgga gtatcagacg ttgctgaaaa taaatcccaa cgaccgcgac gcgcgaacga
      601 aattgaggct tatccttgac caaaaccatg ggaaccccgc ccgctgaatc aaggggctcc
      661 ccctccccaa aaacggacgc tgtccttgtc cgccttgcgc ccctggccgt cgtttttgtc
      721 acgatccttt tcttttggcc cgtttttcag aacggttttg tcaattggga cgacgacgtc
      781 aacttcttga acaacccgca ttatcggggg ctggggtgga tacatcttcg atggatgtgg
      841 acgtcgttcc atatgggtca ttacatgccg gtgacctgga tgagtttggg gctggaccat
      901 gtcctctggg gaatgaatcc ggccggatat catctcacca gtcttctctt gcacgccgtc
      961 aatgccggtt tgttttattt tctcgccctt cggcttttgg ggccagcgga cggcggggaa
     1021 cctcccggcg cggcgccgtt gcgcttcttt gcggccggtt tttccgccct cttcttctcc
     1081 cttcacccgt tgcgcgtgga atccgtggct tgggtgacgg aacggcggga tgtggtatcc
     1141 gggttgttct gcctggtgag cctcctcttc tatcttcgct acgtcgcttc gccgtcgggc
     1201 ggccgcgccc gcttcgtgaa ttacatcgcc agtttcgttt cctacgccgt gtgtttgttg
     1261 tcaagggcca atcttttcgt cctgccgctc ctccttcttg tgctcgacgt ttatccgttg
     1321 cgccgttttt ccaagggggt ggccggaagg gatcggctct tttgggaaaa ggtcccgttc
     1381 gcgttgatcg cggcggcggc tgtcgtttgg gggctggaag gattgctgca ggttaattcc
     1441 tccgtgtcgt tcgcccggca tgggattttc cagcgcctgg cgctggcgtc tttcagtgcg
     1501 gtcttttatg tggggaagac ggtttttccg ttgcgtctgt cgcccctgta tgaaatcccg
     1561 ttgaggttct ccccgtggga ggcgcgtttc gtcgtgagcg gggccgtcgt cgtccttctc
     1621 tcctggcagt tgtggcggcg gcgtcgccgt tggcccgcgg gggtggccgc ctggatttcc
     1681 tatctgcttc tgatcgcccc cacctcgggt cttttccagc gggggatcca gatcgcggcc
     1741 gatcggtaca gttacgcctc ctgcgggggg tgggcgcttc tggccggggc ggccctgcga
     1801 tgggccatgc aaaaattccc ttcggttgcc tggcgccggg gtgtgcttgc cggcgccgcg
     1861 gcggtgtcgg cggtgctggg ggcgtcgacg tgggcccaaa cgcatgtctg gcgggattcg
     1921 gaaacgctct ggcggcatgt gctgcgcgtt caaccccatt ccgccgtggc acactacaat
     1981 ctgggtgtca ttctctcgga acaacatcga gaggacgaag cgcttcgaca ctgggaggag
     2041 accctgagat atgacccggg ggatgccgat gcctatgtcc aggtggggat actttatgag
     2101 gagcgtggtc ggctctcgga ggcggcagaa ctttataaaa ccgcgctccg ctggaaaccg
     2161 gaatccgtgg aagcccactt caatttgggc atccttctag gcaaagaggg gaatttgaaa
     2221 ggagcggaga tgcacctcca tcgggcgttg ctgctggccc ctcgaacggc cgtcgtctgg
     2281 cacagtttgg gccgggttct tgaacggcag aataaatttg atttcgcggc ggaatgtttt
     2341 cgccgggcgc tctctttgga gcccgcccga gccgacagcc gggacgctct taacgacctt
     2401 cttgaaaagc atccggcaaa gaagtagact tttccctcat tcggcaaatg catcggcggg
     2461 ctgcggatgt gacaccatga gttcatcggg cggcggttct tgtgcgccgc tttctccttc
     2521 cgacagagag caacatcgga ttttcccgcg aacatccata cgtaggagga ggaataacct
     2581 tgccagagct tcgtcgtgat cctctcatcg gtcgttgggt catcatctcg acggaaagaa
     2641 aacgccgtcc gagcgatttc atgcatgccc gggaaagcca ggggagcgtc ccctgtcctt
     2701 tttgtcccgg caacgaggaa aagacgccgc cggagatcag cgcctacagc tcccccgggc
     2761 gccttcccaa ccggccgggc tggtgggtgc gcgtggttcc caacaagtac cccgccctgc
     2821 gggtggaggg acagatccat cgcaccggcg accatcttta cgacaagatg gacggtttgg
     2881 gcgcgcacga agtcatcatc gagaccgagg atcacggcaa acagctcgag gacctcagcg
     2941 acgagcgcct ccaggacgtt ttctggacct accgggaccg catcaacgac ctgaaaaaag
     3001 acccgcggct ggagtacatc ctcatcttca aaaatcgcgg cgtcgccgcc ggcgccagcc
     3061 tggcgcatgc ccattcgcag ctcatcgcca cgcccatggt ccccatccgg gtcaagcagg
     3121 agatgcgcgg cgcggatgat tactacgaac gccgcggtcg ctgcattttt tgcgacatgg
     3181 tccagcagga gttggcccaa aaagtgcgcg tggtggatga gaacccgggg ttcgtcgcca
     3241 ttacgccgtt cgccagccgg ttccccttcg agatcagcgt gctgccgaag aaacaccggt
     3301 ccgatttctc ggagatcccg aaatcggagg tcatcgaatt gggccgcatg atcaagagcg
     3361 tgttccagcg cctcaaccgc gttctcgaca acccgtccta taatttcgtc ctgcatgatt
     3421 cgccgctgaa agactctcac ctcgagtact atcactggca tatcgagatc atgcccaaac
     3481 tctcgcagac cgccggtttc gagtggggca ccgggttcta catcaatccc accccgcccg
     3541 aacaggccgc cgaatacttg aaggaaatcg aaaccatcgc cgggtagttc tccgtccgct
     3601 gatccgttcc gcgtgaaaat cctcctcgcc gccagtgaag ccgtcccgtt ctgcaagacg
     3661 ggcggcttgg gggacgtggt cggctccctc ctttccgcct tgcggggcct ccggcacgac
     3721 gcccgcctcg tcttgcccaa acaccgcgcc atcaacgaaa aacggttcgg cctccgccgc
     3781 ctcccgtatc gactcaccct tccgttgggc gacggcgacg aaacggccgc tctgtgggaa
     3841 gcgcgccttc accgcaccgc gccggtctat ttcgtggacg cgccgaaata tttcgaccgg
     3901 gccgggctct atgtcgacga gaccggccgg gacttcgccg acaacgacga ccggttcatc
     3961 ctcttctccc gcgccgtcct cgaaaccgcc aaggccgtgg atttccgtcc cgacgtcctc
     4021 cacgcgcacg actggcagac cggcctggcg ccggcctatc tgtccaccgt ctaccgccac
     4081 gacgcttttt tcatccccac cgccgcctgc ttcaccgtcc acaacatggc ctatcagggc
     4141 ctttttccga aagacgccct tttccgggcg ggcttctcct g
//
LOCUS       DAOTFD010000218         4142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_15847_length_4142_cov_3.757034, whole genome shotgun sequence.
ACCESSION   DAOTFD010000218 DAOTFD010000000
VERSION     DAOTFD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15847_length_4142_cov_3.757034"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4142
                     /locus_tag="P5079_10055"
     CDS             <1..>4142
                     /locus_tag="P5079_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30363.1"
                     /translation="LLDQMLGVIQHLEDIEQALIAKGLDGLRATLAEAGLSADQIEPL
                     LGFLKAGSIDWRNGSFSVGRAADGSTLINMSLEFRDASGKNLGNFVIGFDADKGGFGY
                     YWADLPMDDARVQALLSGMLGFDIAGLSPEELAGLIQENDEFAAFLVQVLGLKDVDAL
                     RALDPTTLQNKLLNAVDTLAQAAQGGDTAAAAQLAQLANYVGVNIVDFERLRSALSAA
                     LGDLSTVLDSLDMNQLKAIAGLLSIGDYDKLTPDALKEAILGAVTADGFSLDALADGS
                     IQRLAALLLPGYNPGDYLIGIAAIKTRLTSAILQNDLTLLAQRDDAFAGFLAKLLNTD
                     VASVRAMSGPALKDAVLQALDGMTAEQAAVLLGDEAVSSFVTAMVGVVPANATDQDKL
                     KYIMGSFAVYLGVTVTDIQQLRSDLSNFISGRVDAYISDLVTKAKFNDPSAIQKLQDL
                     ASVLGVDDIETLIKDKKFDQLGSAVKAALHDSNRIAGLDDDELQTLAGLLEMPLPLQG
                     LSEVRTALRGRIAALTSKLAGEVMDDMDFGGLLGDFLKDVLSNGGRLDFSEGKLTLGT
                     GADGKMIFSALTPIYDASGRKIGDFVIGWDSKNVGFSYAVKFELDHPAVRAFLFDLFD
                     VNSFEELEQVLKDALAEQTFTYRDATGQHTINFAQLLTLLGGDIDFSEGELVIGVDGD
                     GEQVFSVSFEMVRDGMRIGTYNVQYDGDEGGLSYTAVFEGTVPVVRNFLNKLVPLGTS
                     SPIPFGRIDRNNAQFMIGKSAGGDAFWRIVYTLVDDQGHKVGRYVVGMSGNKVDSYAS
                     YDLSNPYVQEAIAAGRLNPPSPTQPEVGELDMTSGTYIVGVSGGETVDRVTYNIIDPS
                     TGEVIGARTDGRTLTETQGWQPDHYSSYDLSKSLVQQAISSGILKPPASNIGFAYGNI
                     PPPPPDRPRPDDFLNRQARWGEGEGDYFDQEAYAAAMESYQEAVAAWEAKYHMSASAW
                     EARYGDAWRRQQGSGGEPPVDMDAGIGVEKEDEGTYTVGVMAGKAVNFVVRNIYDFRG
                     NKIGTHTFGILKGKLNNFRTYNLKSAFVQRAILSGQLRMPTSGPAQQAGPGGVVPPKP
                     PAKPEFRLPKSFFSEDAWEAAYTAYEKKLEDWRASIQEWERLYGMRASDWEGLYGASV
                     QGDGKIPAGHINTSAAPIGDNIEGTYTVGIVDGVSTAIMEWDVIDDRGFTVEHHERGW
                     KDGSGAFALDRYGYSHDEFGRVTGYTEHGWNEGNGYYEDIRSNIGYDAYGRVYSYDQI
                     YSRNGGEPARAHVSAILYDSKGREISKTISVTWTNSHYGLIPGLSDGKHTSTDKDGKS
                     NQAGGWINGSATVTISYKYNESNQVVDVRVAGTVNASNPALAGKKGYSTHFNFSYHQY
                     NM"
BASE COUNT          866 a         1163 c         1297 g          816 t
ORIGIN      
        1 ctattggatc aaatgctggg cgtcattcag catttggaag atatcgagca ggcgctcatt
       61 gcgaaaggat tggacggttt gagggccacc ttggccgagg cgggactctc ggccgatcaa
      121 atagagccgc tgctgggctt cttgaaagcg gggagcattg actggaggaa cggctcattt
      181 tccgtcggcc gtgcggcgga cggcagcacg ctcatcaaca tgtccctcga attcagggac
      241 gcctccggga agaacctggg gaatttcgtg atcggttttg acgcggacaa ggggggattc
      301 ggttattatt gggcggacct tcccatggac gatgcccgcg tgcaggcctt gctcagcggc
      361 atgttgggtt ttgacattgc cggcttatcg cccgaagagc tggccggact gatccaggaa
      421 aacgacgagt ttgcggcgtt cctcgtgcag gtcctgggcc tgaaagacgt ggacgctttg
      481 cgggcgctgg atccgacgac cctgcagaat aaactcttaa atgcggtgga tacattggct
      541 caggccgccc agggcgggga cacggcggcc gccgcgcaac tggctcaatt ggcgaattat
      601 gtcggcgtca acatcgtgga tttcgaacgt ctgcgttcgg ccttgagtgc cgctctcgga
      661 gacttgtcga ccgtcctgga ttccctggac atgaatcagc tcaaggcgat cgccggcctc
      721 ctgagcatcg gggattatga caaactgacg ccggacgcat tgaaagaggc catcctcggg
      781 gccgtgacgg ccgacggttt cagtctggac gctctggcgg acggtagtat ccagcgcttg
      841 gcggcccttc tccttccggg atacaatccc ggcgattatc tcatcggcat cgccgccata
      901 aaaacccggt tgacctccgc catcctccaa aacgatttga ccctcctggc tcaaagggac
      961 gatgctttcg ccgggttcct ggccaaactc ctcaacacgg atgtggcgtc ggtgcgtgcc
     1021 atgtccggtc cggccctcaa agacgccgtg ttgcaggcgt tggacgggat gacggccgag
     1081 caggcggccg tacttttggg ggacgaggcg gtgtcttcct tcgtgacagc catggtgggg
     1141 gtggttcccg cgaacgcgac cgaccaggat aaactgaagt acatcatggg atcgttcgcc
     1201 gtctatttgg gcgtcacggt gacggacatc cagcaactcc ggagcgattt gtccaatttt
     1261 atatcggggc gggtggatgc ctatatcagc gatctggtga ccaaagccaa attcaacgac
     1321 ccgtccgcca tccagaaact gcaggatctg gcgtccgtgc tgggcgttga cgacatcgag
     1381 acgctgatca aggataaaaa gttcgaccag ctgggcagcg ccgtgaaggc ggccttgcat
     1441 gattccaacc gcatcgccgg tttggatgac gacgaattac agacactggc aggccttttg
     1501 gaaatgccgc ttccgttgca gggtctctcc gaagtccgga cggccctgcg cggccggatc
     1561 gccgctttga cttcgaaact ggcgggcgaa gtgatggatg acatggactt cggcgggctg
     1621 ttgggggatt tcttgaaaga cgtcctctcc aacggtggtc ggctggattt ttccgaaggg
     1681 aaactgacgt tggggaccgg cgccgacgga aaaatgatct tcagcgcctt gacgcccatt
     1741 tacgacgcct ccggccggaa gatcggggac ttcgtcatcg ggtgggattc caagaacgtc
     1801 ggtttctctt acgccgtcaa gttcgaactg gaccatccgg cggtccgggc gttcttgttc
     1861 gacctgttcg acgtcaacag cttcgaggaa ttggagcagg tgttgaaaga tgcgttggcc
     1921 gagcagacgt ttacctaccg cgatgccacc ggacagcaca ccatcaattt cgctcagctg
     1981 ttgaccttgt tggggggtga catcgatttc agcgaaggcg agctggtcat cggcgtcgac
     2041 ggggatggag aacaggtttt cagcgtgagc tttgagatgg tccgggacgg gatgcggatc
     2101 gggacgtaca acgtgcaata cgatggagac gagggcgggt tgtcctatac ggccgttttt
     2161 gaaggaaccg ttccggtggt tcgaaatttc ctgaacaaac ttgtcccgct ggggacatct
     2221 tcgccgatcc ctttcgggcg gatcgatcgc aacaacgccc agttcatgat cgggaaatcc
     2281 gccggcgggg acgccttttg gcgcatcgtc tacaccctcg tggacgatca gggacataag
     2341 gtggggcgtt atgtggtggg catgtcgggg aacaaagtgg attcctacgc gagctacgat
     2401 ctttccaatc cctatgtgca ggaggccatc gcggcgggcc gcctgaatcc gccttcgccc
     2461 actcagccgg aagtcggcga gctggacatg acctccggca cctacatcgt gggcgtgtcg
     2521 ggcggggaaa ccgtcgacag ggtcacctac aacatcatcg atccgtcgac aggagaagtc
     2581 atcggggccc gaaccgacgg tcggaccctg accgagaccc aggggtggca gccggatcat
     2641 tattcttcct acgacctttc caaatcgctc gttcaacagg cgatttccag cgggatcctg
     2701 aagccgcccg cctccaatat cggtttcgct tatggaaaca tcccgccgcc tccgccggac
     2761 cgtcctcgcc cggacgattt tctcaaccgg caggctcgtt ggggagaggg cgagggtgat
     2821 tatttcgatc aggaggccta cgccgccgcc atggagtcat accaggaggc cgtggccgcg
     2881 tgggaggcga aatatcacat gtccgcctcg gcttgggagg cgcgttacgg cgacgcctgg
     2941 cgccggcagc aggggagcgg cggcgaaccg ccggtggaca tggacgccgg catcggcgtt
     3001 gaaaaagagg acgagggaac ttataccgtc ggtgtgatgg ccggcaaggc cgtgaatttc
     3061 gtcgtaagga acatctacga tttccgtgga aacaagatcg gcacccatac cttcggcatc
     3121 ctcaaaggca agctgaataa tttcaggacc tataacctga agagcgcctt cgtgcaaaga
     3181 gccattttgt cgggacagct gcggatgccc acgtcggggc cggcgcagca ggcgggcccc
     3241 gggggcgtgg tgccgcccaa gcccccggcg aaacctgagt ttcgtctgcc caagtcgttt
     3301 ttctccgaag acgcctggga agccgcttat accgcctatg agaaaaagct ggaggactgg
     3361 agggcgtcta tccaggaatg ggaacgtctt tacggaatga gggcgtcgga ttgggagggg
     3421 ctctatgggg cttccgtcca gggcgacggg aaaatccccg ccggacacat caacacctcg
     3481 gcggcgccga tcggcgacaa tatcgaggga acctacaccg tcgggatcgt ggacggcgtc
     3541 tctaccgcca tcatggaatg ggacgtaatc gacgaccgtg gtttcaccgt cgaacatcat
     3601 gagcgcggct ggaaagacgg cagcggggct ttcgccctcg atcgatacgg ctacagccat
     3661 gacgaattcg gccgggtcac gggatacacc gaacacggtt ggaacgaggg caacgggtac
     3721 tatgaggata tccggagcaa catcggatac gacgcctacg gtcgtgtgta ttcctacgac
     3781 cagatctatt cccgcaacgg cggcgaaccc gcccgcgccc acgtttcggc gatcctttat
     3841 gacagcaagg gacgggaaat atcgaagacg ataagcgtca cctggaccaa ttcacattac
     3901 gggctcatcc cgggcctttc cgacggaaaa cacaccagca cggacaagga cgggaaaagc
     3961 aaccaggccg gggggtggat caacggcagc gccaccgtca ccatctcgta caaatacaac
     4021 gagagcaacc aggtcgtgga cgtccgcgtc gccggcaccg tcaacgcctc caacccggcg
     4081 ttggccggca aaaaggggta ttcgacacac tttaatttca gttaccacca atacaatatg
     4141 ca
//
LOCUS       DAOTFD010000219         4100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16078_length_4100_cov_3.832633, whole genome shotgun sequence.
ACCESSION   DAOTFD010000219 DAOTFD010000000
VERSION     DAOTFD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16078_length_4100_cov_3.832633"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..162
                     /locus_tag="P5079_10060"
                     /pseudo
     CDS             1..162
                     /locus_tag="P5079_10060"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
     gene            172..>405
                     /locus_tag="P5079_10065"
     CDS             172..>405
                     /locus_tag="P5079_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30364.1"
                     /translation="MSYGSTGTGSVYTYLLPIVLTSSGAGGAFFTSELTLTNRSGRTL
                     NLTFRAKGSFEASSTYSLPPGQQIHPDVFAFLQN"
     assembly_gap    406..415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <416..937
                     /locus_tag="P5079_10070"
     CDS             <416..937
                     /locus_tag="P5079_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30365.1"
                     /translation="RTTSLRIEVAGADNLVQFGAQVRVTTPATPDLRTKGVIGRFGLA
                     FPATPLGRGGLPLLPAREQVPNTELMTYKVVALARLVAPESNIPATTALATLNRTHGR
                     ELGLSRGANVVMPNLTPPRYRVLYEIYPGKACVQETATQCGFCLKERIHRLGRHVGAG
                     PGSRRRSASALPA"
     gene            1047..1131
                     /locus_tag="P5079_10075"
     tRNA            1047..1131
                     /locus_tag="P5079_10075"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1081..1083,aa:Tyr,seq:gta)
     gene            1390..3069
                     /locus_tag="P5079_10080"
     CDS             1390..3069
                     /locus_tag="P5079_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY30366.1"
                     /translation="MSDTPVKEEMDMEQLLKESLPPNKAGEIVTARVVEVTPEGLMVD
                     VGLKVEGFVPKSEFSSHAPMPAKGETFPALIKRMGGAEGHPLVSWREAKDRLHRDHVL
                     KAKNEQTPLEGVVLRQVKGGLIVDVGMEGFLPASQVDRRPVKDLKPFIGQKVNVFVME
                     MDSKKGGAVLSRRQFLDKEAAARQAETLKTLTEGMVVTGTVTSLTAFGAFVDIGGIEG
                     LLRVSDVAWGRMGKLSSVLKVGQKIEVKVLKYDTATRKISLGRKQLLPHPWEGVEGRF
                     TVGKTVKGKVTSLTPFGAFVELEPGVEGLIHQSEFSWKERWVKPDQFVKVGQEVEVQV
                     VSCARGEEKIGLSLKRASENPWEEAAKLYRPGARVKGTVTHIVPFGAFVRLSIGVEGL
                     LRVSDISWTKTVTHPKEWVTEGQEIEAVVLEVSPAEEKIALGLKQLVEDPFSKYKPGR
                     VVEGKVARFIEHGAIVTLEPDVEAFVHVSEIPSTHRLTHPSEALALEQVVTGQVLKTQ
                     KKSRRIEISIRKYEHDQERKLIKKYAGGKDRITLGEMTAWEGNKEEDVPSA"
     gene            3122..4066
                     /locus_tag="P5079_10085"
     CDS             3122..4066
                     /locus_tag="P5079_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30367.1"
                     /translation="MTVRSEPPAVPPPPKNIPLKNFSGTPWLVFLLVVAVAILWTLGL
                     YRPRSGHFPRAEAEFRPAVERERARAAAQASLEKDPENIAAWIETAVARFEEGPAQYL
                     PALEALERARDLGAVDPRLFYYAAVMYEAVGLPDYAQPDYARYLRHRPDDAEVRLRLA
                     NLQYRLGDYDKAIEEYSRLAAARPRDAVLACNLAAAYREKQRWPEGIELLERVSRDGA
                     PLPDGGYKIWGDLHRGAGDPRRALENYHTELTRQKDSIELAGAMAGAYEQLQDWPQAM
                     DQWKRVAELDPKHRLAREKIRSLQRKIRAAERAGRKGK"
BASE COUNT          754 a         1344 c         1354 g          638 t
ORIGIN      
        1 ctctccgacc ggacgcaggg aggagccgcc agctggagct ggtccttcgg ggacggctcg
       61 acctccacga cgcagaaccc gacgaaggtc tgggggaacc ccggcaccta ctccgtcagc
      121 ctgactgcgg ccaacgccgc cggcagccgc tcggtcacga agggcgtcac cgtgtcgtac
      181 ggctcgaccg gcaccgggag cgtctacacg tacctccttc cgatcgtcct gacgtcgagc
      241 ggcgcaggag gcgccttctt cacctccgag ctgacgctga cgaaccgctc cggccggacg
      301 ctgaacctca cgttccgggc gaagggctcc ttcgaggcct cctcgaccta ctccctcccg
      361 ccgggacagc agatccaccc ggacgtcttc gcgttcctcc agaacnnnnn nnnnncggac
      421 gacgtcgctc cggatcgaag tggccggcgc cgacaacctc gtccagttcg gtgcgcaggt
      481 ccgcgtgacg acgccggcga cacccgacct tcggacgaag ggggtcatcg gccgcttcgg
      541 gctcgcgttc ccggccacgc cgctcggccg cggcggattg ccgctcctcc ccgcgcggga
      601 acaggtcccc aacacggaac tcatgaccta taaagtggtg gccctggctc ggctggtcgc
      661 tcccgaatcc aacatcccgg ccaccacggc gctggccacc ctcaaccgga cgcacggccg
      721 ggaactgggc ctgtcgcgcg gcgccaacgt ggtcatgccc aacctcaccc cgccccggta
      781 ccgcgtcctc tacgagattt atccgggcaa ggcctgcgtt caggagaccg ccacccagtg
      841 cggcttctgc ctcaaggaac gcatccaccg tctcggccgc cacgtgggcg ccggccccgg
      901 ctcccgccgc cgctccgcct ccgccctccc cgcctgacgt cgacacggca tgccgcttgc
      961 ccctaccttt ccggccggct ttcatggtaa aataacccct tcacgttggg tcaccccgcg
     1021 cggtcatccc cgtttcctgg tttttcgcac aggtggcaga gcggtcaaat gcaccagact
     1081 gtaaatctgg cgggcttacg ccctacgaag gttcaaatcc ttccctgtgc atttcttttt
     1141 accgcggcgg gcgatcgacg gccccgcaat acgttcgctg cgacggcgag acctgtaaca
     1201 cgggccccgt tgtgttcggt ggtgggggcc gcccaccatc cgtgctcatg gcggatgggg
     1261 agaagcggcc gtggccccgc cgtcgaaaga tcggcaaaac ggctgtttgt atagtaaaat
     1321 gaaccccttt gggaccgggc cagaggcctt tcgggccgtc tcaagccgga atcctccaca
     1381 ggaatgatta tgagcgatac acccgtcaaa gaagagatgg acatggagca actcctcaag
     1441 gagtcccttc cgcccaacaa ggcgggggag atcgtcaccg cccgcgtggt ggaggtgacc
     1501 cccgagggac tgatggtgga cgtgggcctg aaagtggagg ggttcgtgcc caagtcggaa
     1561 ttttcgtcgc acgccccgat gccggccaaa ggggagacgt tccccgccct catcaaacgc
     1621 atgggcggag cggagggaca tccgctggtg tcttggcggg aagccaagga ccggctgcac
     1681 cgcgaccacg ttttgaaagc caagaacgag cagacgccgc tcgagggggt ggtgctgcgc
     1741 caggtgaagg gcgggctcat cgtggacgtg ggcatggaag ggttcctgcc ggcgtcccag
     1801 gtggaccgcc gtcccgtgaa ggatttgaag ccgttcatcg ggcagaaggt caacgttttc
     1861 gtgatggaga tggacagcaa aaaaggcggc gcggtgctgt cccgccggca gtttttggac
     1921 aaggaggccg ccgcccgcca ggccgagacg ctcaagacct tgacggaagg gatggtcgtc
     1981 accggcaccg tgaccagcct gacggcgttc ggcgcgttcg tggacatcgg cggcatcgag
     2041 gggctcctgc gcgtttccga tgtggcctgg ggccggatgg ggaaactttc ctccgttttg
     2101 aaggtgggac agaagatcga ggtgaaggtc ctcaaatacg acaccgccac ccgcaagatt
     2161 tccctcggcc gaaaacagct tttgcctcac ccctgggaag gcgtggaggg gcggttcacc
     2221 gtcgggaaga ccgtgaaggg gaaggtgacc agcctcactc ctttcggggc gttcgtggaa
     2281 ctggagcccg gcgtggaagg gctcatccac caatcggaat tttcctggaa ggaacgctgg
     2341 gtgaaaccgg accagttcgt gaaggtgggc caggaggtgg aggtgcaggt ggtgtcgtgc
     2401 gcgcggggcg aggagaagat cgggctgagc ctgaaacgcg cctcggagaa cccctgggaa
     2461 gaggccgcca aactctaccg gcccggcgcg cgcgtgaaag gcacggtgac gcacatcgtc
     2521 cccttcggcg ctttcgtgcg gttgtccatc ggcgtggagg ggctcctgcg ggtgagcgac
     2581 atctcctgga ccaagaccgt gactcatccc aaagagtggg tgaccgaagg ccaggagatc
     2641 gaggccgtgg tgttggaggt gagccccgcc gaagagaaga tcgcgctggg gctgaaacag
     2701 ctggtggaag accctttttc caaatacaag cccggccgcg tggtggaggg gaaagtggcg
     2761 cgcttcatcg agcacggcgc catcgtgacc ttggaacccg acgtggaggc gttcgtccac
     2821 gtgagcgaga tcccgtccac gcaccggttg acccatccgt cggaggcgct ggccctggaa
     2881 caggtcgtca ccggacaggt cctcaagacg cagaagaaat cgaggcgcat cgagatttcc
     2941 atccgaaaat acgagcacga ccaggaacgc aagctcatca agaaatacgc gggcggcaag
     3001 gacaggatca ccctcggcga aatgaccgcc tgggagggaa acaaggaaga ggacgtcccc
     3061 tccgcgtgac gccccggccc gcccgaccgg cgggagagac cgcgtttttt ccaaaacgac
     3121 catgaccgtc cgttctgagc cgcccgccgt cccgcctccc ccaaaaaaca tccccctgaa
     3181 aaatttttcc ggcacgccct ggctggtgtt tttgctggtg gtggcggtgg ccatcctgtg
     3241 gaccctgggg ttgtatcgcc cgcgttccgg ccacttcccg cgcgccgagg cggagttccg
     3301 gccggcggtg gaacgggaac gggcccgggc ggcggcgcag gcctcgctgg agaaagaccc
     3361 ggagaacatc gccgcctgga tcgagaccgc ggtggcgcgg ttcgaggagg ggccggccca
     3421 gtatttgccg gcgttggaag cgctcgaacg ggcgcgcgac ctgggcgcgg tggacccgcg
     3481 cctcttctac tacgccgccg tgatgtacga ggcggtgggc ctgcccgatt acgcccagcc
     3541 ggattacgcg cggtacctgc gccaccggcc cgacgacgcc gaggtgcgcc tgcgcctggc
     3601 caacttgcag taccgcctcg gcgattacga caaggccatc gaggaataca gccgcctggc
     3661 cgccgcccga ccccgcgacg ccgtgttggc gtgcaacctc gccgccgcct atcgcgaaaa
     3721 acagcgctgg ccggagggga tcgaactgct ggagcgcgtc tcccgcgacg gcgcgccgct
     3781 gcccgacggc ggatataaga tttggggcga cctccaccgc ggcgcggggg accctcgtcg
     3841 cgcgctggag aactaccaca ccgaactgac gcggcagaaa gacagcatcg aactggccgg
     3901 cgccatggcc ggcgcctacg aacaactgca ggattggccc caggcgatgg accagtggaa
     3961 acgcgtggcc gaactcgatc cgaaacaccg cctcgcccgc gagaagatac gctccctgca
     4021 gagaaaaata cgcgccgccg agcgcgccgg ccgaaaaggg aagtgacgtg aggcgcggtc
     4081 cgcgggtttc tttccgggcc
//
LOCUS       DAOTFD010000220         4092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16123_length_4092_cov_4.499628, whole genome shotgun sequence.
ACCESSION   DAOTFD010000220 DAOTFD010000000
VERSION     DAOTFD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16123_length_4092_cov_4.499628"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="P5079_10090"
     CDS             <1..283
                     /locus_tag="P5079_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY30368.1"
                     /translation="PGPHKIQGIGAGFVPKVLNRSTVDEVVSVKHTDAGEMARRLARE
                     EGILVGISSGAALAAGLEVARRKESEGKTIVVLLPDTGERYLSTWLFTE"
     gene            313..1449
                     /locus_tag="P5079_10095"
     CDS             313..1449
                     /locus_tag="P5079_10095"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRY30369.1"
                     /translation="MEERGIGVVETRHVTFAEPPNELTLESGQKLGPVTVAYETYGRL
                     DEKKSNAVLVTHALSGDAHAAGVHFGEKKPGWWEPLIGPGKAFDTDKYFVICSNVIGG
                     CRGSTGPSSINPRTGAPYALSFPFITIGDMVAAQKKLIDHLGIEKLLCVAGGSMGGMQ
                     ALQWAAAYPDRVRSVIPIATTLKHSPQQIALSEVGRQAILADPAWRGGNYYAHGQPER
                     GLALARMIGHITYMSDKSMEEKFSRKLKSENYRFSFDPEFEVEGYLRHRGDSFVRRFD
                     ANSYLYVTKALDYFDLSGGDLIPEGRPCDIRFLVISFKSDWLYPSYQSRDIVAFLRTR
                     LVDVTYCEINSTYGHDAFLLEFDEETRLIAHFLDKTYDSTKGKP"
     gene            1446..2060
                     /gene="metW"
                     /locus_tag="P5079_10100"
     CDS             1446..2060
                     /gene="metW"
                     /locus_tag="P5079_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRY30370.1"
                     /translation="MTDKQPRLDDRIIAQIVEPGAKVLDLGCGTGDLLALLVREKNVK
                     AQGIELREEAIYACVEKGLSVFHGDIESGLGEYPDKTFDYVILNQSMQEVKDVEFVID
                     EALRVGKKVVVGFPNFAFIKSRIQLFFSGHAPVFVSLPYHWYDTPNVHFLSIDDFKRF
                     CAEKGLVILSAHYLGGDRPVAFWPNLFARDAVFVITKPAPASSR"
     gene            2197..2928
                     /locus_tag="P5079_10105"
     CDS             2197..2928
                     /locus_tag="P5079_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30371.1"
                     /translation="MKLPSVTKTTQRLAPKFRAAAVFVSGLAARGKGAVPRMGKSARW
                     RSCLRAVRSRLRPSAGTVDGLAAALAEGMAEFRRGLEGEAARMSPIFEACAREAAAGC
                     PAVAENAGRMGFALAQSVRESFLIMETLMREMALFGLSPDRSMREMASALAASAGRLP
                     RLLKGTSPSAARRRDLETFRREMAAARTVYEKNLGRLFRRWDAVEVLKEREMHRHLWR
                     MAESLRRLLRPLEEEITSERKGAGA"
     gene            2928..>4092
                     /locus_tag="P5079_10110"
     CDS             2928..>4092
                     /locus_tag="P5079_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY30372.1"
                     /translation="MGKVLNGEVEAKFSEKKVRRVAFLVYASAVIALYFWNPAVSAPR
                     RLIVFHTNDIHGRVSSAPSSGGGEWVGGFPVLAAFLAEQSRPYLLLDAGDFYQGTPEG
                     NLTRGDAAVDGMNAVGYDAAAVGNHEFDDGVENLARLAGAAEFPLLCANVTDRRAPSR
                     LAALRPWVLKEVDGVKVGVFGLISRRTSEMVFAKNVADLTFADEAAAARRAVEELRRQ
                     GADIVIALSHLGYEQADKPPAAEESAADDQTLALSVDGIDLIVGGHSHTPLFAGRRVG
                     KTWVVQAGTGLRWIGRVILSWDGRTKKIVGVRSRLIPLDRDRWGEEPNVAALMRYYRE
                     AASRTLGRDLEEVVGTLQEPLKRNYRGESSSGDWHADVLRAFAGADVAFHNSGG"
BASE COUNT          721 a         1226 c         1399 g          746 t
ORIGIN      
        1 gcccggcccg cacaagatcc agggcatcgg cgcggggttt gtgccgaaag tcttgaaccg
       61 gtccaccgtg gacgaggtcg tctcggtgaa acataccgac gcgggcgaga tggcccgccg
      121 gctggcgcgc gaggaaggga tcctcgtcgg tatctccagc ggggccgcct tggcggcggg
      181 actggaggtg gcccgacgga aggaatcgga agggaagacc atcgtggtgc ttttgccgga
      241 taccggcgaa agatatcttt ccacgtggct ttttacggaa taaaatagac atccccggga
      301 ggggaagaga agatggaaga gagaggcatc ggagtcgtcg agacgcgcca cgtcacgttc
      361 gctgagccgc cgaacgagct gacgttggag tcgggtcaga agctggggcc cgtcaccgtg
      421 gcgtatgaga cctacggccg cttggacgaa aaaaaatcca acgcggtgct ggtgacgcac
      481 gccctttcgg gggacgctca cgccgccggg gtgcatttcg gcgagaagaa gcccggctgg
      541 tgggaacccc tcatcgggcc cggcaaggcg ttcgataccg acaagtattt cgtcatctgt
      601 tccaacgtca tcggcggctg tcggggcagc acgggcccct cttccatcaa cccgcgcacc
      661 ggcgcgccgt acgcgctctc cttccccttc atcaccatcg gcgacatggt ggcggcgcag
      721 aagaaattga tcgatcacct gggcatcgag aaactcctgt gcgtggcggg cgggtccatg
      781 ggggggatgc aggcgctcca atgggccgcc gcgtatccgg accgggtgcg gtccgtcatc
      841 cccatcgcca ccacgctcaa gcattcgccc cagcagatcg ccctcagcga ggtgggtcgt
      901 caggccattc tggccgaccc cgcctggcgc ggcggcaact actacgcgca cggtcagccg
      961 gaacgcggcc tggccctggc gcggatgatc gggcacatca cctacatgag cgataagtcc
     1021 atggaggaaa aattttcacg caaactcaag agcgagaact accgtttctc gttcgacccg
     1081 gaattcgagg tggagggata cctccgccat cggggcgata gttttgtccg gcgtttcgac
     1141 gccaactcct atctctacgt gaccaaggcc ttggactatt tcgacctttc cggcggggat
     1201 ctgatccccg aaggccggcc gtgcgacatc cggttcctgg tcatctcgtt caaatcggac
     1261 tggctctacc cgtcgtatca gtcgagggac atcgtcgcgt tcctgcggac gcgcctggtg
     1321 gacgtgacct actgcgagat caactccacc tacgggcacg acgctttctt gttggagttc
     1381 gacgaggaaa cccggctgat cgcgcatttt ctggataaga cctacgattc cacgaagggc
     1441 aagccatgac cgacaaacaa cccagactcg acgaccggat catcgcccag atcgttgaac
     1501 ccggggccaa ggtgctggac ctcggttgcg gcaccggcga cctgttggcg ctgttggtgc
     1561 gggagaagaa cgtcaaggcg cagggcatcg aactccgcga agaggccatc tacgcctgcg
     1621 tggagaaggg gctcagcgtt ttccacggcg acatcgagag cggcttgggg gaataccccg
     1681 acaagacctt cgactacgtc atcctgaacc agagcatgca ggaggtgaag gacgtggagt
     1741 tcgtgatcga cgaagccctg cgcgtgggca agaaggtcgt ggtgggattc cccaacttcg
     1801 ctttcatcaa gtcgcgcatc caactttttt tctccggcca cgcgccggtg ttcgtgtcgc
     1861 tcccctacca ttggtacgac acccccaacg tgcatttctt gagcatcgac gacttcaagc
     1921 ggttttgcgc ggaaaagggg ctggtgatcc tgagcgccca ttatttggga ggcgaccgac
     1981 cggtcgcctt ctggcccaac cttttcgccc gcgacgctgt cttcgtcatc acgaaaccgg
     2041 cgcccgcctc ctcccgataa aatccgtccg tcccctcccg gcgaatgcct tcgatttttc
     2101 gggccgggcg gctaattcaa ttgaagacgg acctctgaat atccgggcga tggtaaattg
     2161 acgggccgtg gcccgcgtgt tagaatggcg gtagcgatga aacttccgtc cgttacgaaa
     2221 acgactcagc ggctggcgcc gaaattccgg gcggccgctg tttttgtttc cggcctggcc
     2281 gcgcgcggga aaggggctgt ccctcggatg gggaaatcgg cgcgttggcg gagctgcctg
     2341 cgcgccgtgc gaagccgcct gcgtccgtcg gccgggacgg tcgacggctt ggccgcggcc
     2401 ctggccgagg ggatggctga atttcgtcgg ggtttggagg gcgaggcggc gaggatgtcg
     2461 ccgatattcg aggcctgcgc ccgcgaggcg gcggcgggat gtccggccgt cgccgagaac
     2521 gccggccgga tggggttcgc cctcgcccag agcgtgcggg aatcgttttt gatcatggag
     2581 accctgatgc gggagatggc gctcttcggt ctttctccgg atcggtcaat gcgggaaatg
     2641 gcctcggcgc tggccgcgtc ggcgggacgc cttccgcgac tcttgaaggg aacgtcgccg
     2701 tccgccgcgc ggcgccggga tttggaaacg tttcgacggg agatggcggc cgcgaggacc
     2761 gtctatgaga aaaacctcgg gcgcctcttc cgtcgttggg acgccgtcga ggtgttgaag
     2821 gaacgcgaga tgcaccgcca cctctggcgg atggcggaaa gccttcggcg gctccttcgt
     2881 ccgctggaag aggaaataac gtcggaacgc aaaggggccg gcgcgtgatg ggaaaggtct
     2941 taaacgggga agtggaggcg aagttctctg aaaaaaaggt ccgccgggtc gcgtttttgg
     3001 tgtacgcctc ggcggtcatc gcgctttatt tctggaaccc cgcggtttcc gcgccccgcc
     3061 gcctcatcgt tttccatacc aacgacatcc acggccgggt atcttccgcc ccgtcgtccg
     3121 gcggaggaga atgggtcggc gggttcccgg tgttggccgc tttcctggcg gaacaatccc
     3181 ggccgtattt gttgctggac gcgggggatt tctatcaggg cacgccggaa gggaacctca
     3241 cccgcggcga cgccgcggtg gacggcatga acgccgtggg atacgacgcg gccgcggtgg
     3301 gaaaccatga attcgacgat ggggtggaga acctggcccg cctggccggg gcggcggaat
     3361 tcccgttgct ctgcgccaac gtgacggacc ggcgcgcgcc gtcgcgcttg gccgctctcc
     3421 ggccgtgggt gttgaaggag gtcgacggtg tgaaggtggg ggtgttcggg ctcatctccc
     3481 gccgcacgtc ggagatggtt ttcgccaaga atgtggccga cctgacattt gccgacgagg
     3541 cggccgcggc ccggcgggcg gtggaggaac tgcgccgcca aggggccgac atcgtcatcg
     3601 cgctcagtca tctgggatac gagcaggcgg acaagccgcc ggcggccgag gaatccgccg
     3661 ccgatgacca aacgttggcg ctgtccgtgg acggcatcga cctgatcgtg ggcggccaca
     3721 gccacacgcc gttgttcgcc ggtcgacggg tcgggaagac gtgggtcgtt caggcgggca
     3781 ccggcctgcg ctggatcggc cgggtgatcc tgtcgtggga cggccggacg aaaaagatcg
     3841 tgggcgtccg cagccggttg attccgctcg atcgggaccg ctggggagaa gagcccaacg
     3901 tcgccgcgtt gatgcgttat taccgcgagg cggcgtctcg gacactcggg cgcgacttgg
     3961 aggaggtggt gggcacgctc caggagccgc tgaaacggaa ctatcggggg gaatcctctt
     4021 ccggggactg gcacgcggac gtcctgcggg ccttcgccgg cgccgatgtg gcgtttcata
     4081 attccggcgg ca
//
LOCUS       DAOTFD010000221         4073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16221_length_4073_cov_3.569686, whole genome shotgun sequence.
ACCESSION   DAOTFD010000221 DAOTFD010000000
VERSION     DAOTFD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16221_length_4073_cov_3.569686"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..3938
                     /locus_tag="P5079_10115"
     CDS             24..3938
                     /locus_tag="P5079_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRY30373.1"
                     /translation="MLKLAGRAVFRSGDFKVVPLPGTNVLAFTRTAGNQRALVATNFS
                     NDWQRVVIPLPQEWRNRIQRGRTYTLDDLYFDSKPAVEQDRPGVSPRYTFTGDELLDN
                     GIYVDLAPWDSHVFSNLKAENKFEKLGKIIFGTDIKTPSPAGAWHVGDAVRLLGWTGR
                     FTFYWTTFGTVLLPRVLNWFQDKLMDSWLPFIFIKIPRSLGNLVPTDLLKMAYGLIFY
                     RYAIIVGYYVMPVIYSAVFFGHRRPFNVSAPEEVKMSLLHKRAIKHLVAFQSNDQRRA
                     VFDMVKRVEPTAEWVESSEDYLVVQLTRGVKLRVVIALSHVPSHIKRPDLHALATTAE
                     MPIPFNVATGRTYIVTLQTALLRYPENALYTVLEHEIEEALIHTQEGIDFGGAHARVA
                     EMQGPEAVERVAEAIRQDNEKTAETDAKKPALITLEGASGTGKSTTAKALSQQMGAAY
                     LSMGQIYRTLTWLARRENIDLAAPEAQEKLAALGQTLHYETVDGELHLFAGDVDTVTI
                     DSEIRAREVESTVPRVAELVRETVVAPMVSRLLSTLLVRGMPVVLEGREEIRAIQPPE
                     GRVFNLRLVAPPEVRAERRLGDWLKKASLGELFMKFFPGVTLPSEPEMRARLLNLVQE
                     DLTTRDEADLRQVSDTLKKMGVTMIDTARLDISQAVRAITDLVSGKLPLAGTWSVRSA
                     ELLAKEVLPALRDGTLAVTSAGLSMLPQIWDRLEGALVDRETAWKAYADKVSGLPYEV
                     QAQILHEVASAQEHRQSFSQDQQTRVVELLEAVTGVAAVTIDSFDPTTQTLALTIAPQ
                     TGQPFALRVFLSDVPSFRKYPEMHVMGTIRGPTATDNAYEISLQQLITKMPAESAVQE
                     TIVFHEIREVVLIQSGMNPEAAHQKTLSEQGESAVERVTLALRKEAVKLDPAVQSLRV
                     RANELRTEQPAPVKIDIPELALPLPVSQERYLEDMPVRMRLGQPAGLIDLVNLSEFFA
                     GPETARLIDARGIAAQLDLRGLPFADAEERRSLVEKLIALQESAESSVLEGVTRTAES
                     LPEGRPKAREAVSDLIVEAVIAAGREEGQLAAQKNANVVSAYRASLQTRFEKLLNDLL
                     AIGATTGLRAEDEVVAALAAVYEKAFLQGAQRFEYLADEQVLAPKEGETASKLDVGMF
                     DVVGSLNDPQNADTVAKIVTKAGLYRSDLGKFAAAIMTDGLTATADEVRRDVLARLAR
                     SGFSKEECDKISANLLIVPTLNAVVGGKIDLDIMFNTVQEHFKGRIGQISLFTSNPAR
                     FREPWRAQIAWVLYLVMPHGVLQASKSIEEGLRAVRVLNTQA"
BASE COUNT          804 a         1313 c         1229 g          727 t
ORIGIN      
        1 aattccgacg gtatttctcc aacatgttga aattggcggg gcgggccgtg ttccgtagcg
       61 gtgattttaa ggtggtcccc ctgccgggaa caaacgtgct ggcttttaca cggaccgccg
      121 gaaaccaacg cgctcttgtg gcgacaaatt tttccaacga ctggcagaga gtggtcatcc
      181 cgctgcctca ggaatggcgc aaccgcatcc aacgcggaag gacctatacg ttggatgacc
      241 tctattttga ttccaagccg gccgttgaac aggatcggcc gggagtgtcg ccgcgctaca
      301 cattcaccgg cgatgaacta ttggacaacg ggatatacgt tgacctggcc ccctgggact
      361 cgcatgtgtt ttccaacctc aaggccgaga ataaatttga aaaactcggg aagataattt
      421 tcggcacgga tataaaaacg ccgtctccgg ccggcgcgtg gcatgtggga gacgcggtcc
      481 gattgttggg ctggacgggg cgtttcactt tctactggac cacctttggc acggtccttc
      541 ttccgcgcgt cctcaactgg ttccaggaca aattgatgga ctcgtggctg ccgttcatct
      601 tcataaagat cccccggagt cttggcaatc tggtgcccac cgacctgttg aaaatggcct
      661 atgggctcat cttctaccga tacgcgatca tcgtcggcta ttacgtcatg ccggtcatct
      721 actccgccgt tttcttcggc caccggcgtc cgttcaacgt ctcggcgccg gaagaagtca
      781 aaatgtccct gctccacaaa cgggccatca aacacttggt ggcgttccag tccaacgacc
      841 aacggcgggc ggtgttcgac atggtgaaac gcgtggagcc gacggccgag tgggttgagt
      901 cttcggagga ttatctggtc gttcaattga cgcgcggggt caagctccgc gtcgtcatcg
      961 cgttgtcgca cgtgccgtcg cacatcaaaa ggccggacct ccacgcgctg gccaccacgg
     1021 cggaaatgcc gatcccattt aacgtcgcca ccggccgcac ctatatcgtt acccttcaaa
     1081 ccgccctcct ccgttatccg gaaaacgccc tgtacacggt cctcgaacac gagatcgagg
     1141 aggcgctgat ccacacccaa gagggcattg atttcggcgg agcccatgcc cgcgtcgccg
     1201 agatgcaagg gcccgaagcg gtggagcggg tggcggaagc catccggcag gacaacgaaa
     1261 aaacggctga gacggacgcc aaaaaaccgg cgttgatcac cctggaaggc gcgagcggga
     1321 ccgggaaatc caccaccgcc aaggcgctca gccagcagat gggcgccgcc tacctctcca
     1381 tgggacagat ctaccgcacg ctcacctggc tcgcccgtcg ggaaaacatc gatctcgcgg
     1441 cgccggaggc ccaggaaaaa ctggcggcgt tgggccagac cctgcactat gagacggtgg
     1501 atggggaact ccatcttttc gccggcgacg tggacaccgt caccatcgat tccgagatcc
     1561 gcgcccggga agtggaatcc acggtcccgc gggtggcgga actcgttcgg gaaacggtgg
     1621 tcgctcccat ggtttcccgg ttgttgagca cgttgctggt gcggggcatg ccggtggttc
     1681 tggaaggccg cgaggagatc cgggccattc agccgcccga aggccgcgtt ttcaacctcc
     1741 gtctcgtggc tccgccggaa gtccgcgccg aacggcgtct cggagactgg ctgaagaagg
     1801 catccctcgg ggagttgttc atgaaattct tccccggcgt cacgctcccc tcggaacccg
     1861 agatgcgggc ccgactgctg aatttggtcc aggaagacct caccacgcgg gacgaggcgg
     1921 acctgcggca ggtctccgac acgttgaaga agatgggcgt cacgatgatc gatacggccc
     1981 ggctcgacat ctcgcaggcg gtccgcgcca tcaccgatct tgtttccgga aaactcccct
     2041 tggccggcac atggagcgta cggtcggcgg aactgttagc caaggaagtg ctcccggccc
     2101 tccgggacgg cacgttggcc gtcacctcgg cggggttgtc catgctccct cagatatggg
     2161 accggctgga aggcgccctc gtcgaccggg aaaccgcctg gaaagcgtat gcggacaagg
     2221 tctccggcct gccgtacgaa gtccaggcgc agatcctgca cgaagtggcg tcggcgcaag
     2281 aacaccggca atccttctcc caggaccagc agacgcgcgt cgtcgagctt ttggaggccg
     2341 tcaccggcgt cgccgccgtg accatcgatt cgttcgaccc gacaacccag acgctggcgc
     2401 tcaccatcgc cccgcaaacg ggccaaccgt tcgccttgcg ggtgttcctg tccgacgtgc
     2461 cctctttccg aaaatacccg gagatgcacg tcatgggcac catccgcggc cccaccgcga
     2521 cggacaacgc gtacgagatc agtcttcagc aactcatcac caaaatgccc gccgaaagcg
     2581 ccgtccaaga aaccatcgtt ttccatgaaa tccgcgaagt cgtcctcatc cagtccggga
     2641 tgaaccccga agcagcccat caaaagaccc tgtccgaaca gggcgaatcc gccgtggagc
     2701 gcgtcacgct ggccctgcgc aaagaggcgg tgaaactcga cccggccgtc caatcgctgc
     2761 gggtccgggc gaacgaactg cgcaccgaac agccggcgcc cgtgaagatc gatatccccg
     2821 aactggcgct gcctctgccg gtgtcgcagg aacgttatct ggaggacatg ccggtgcgga
     2881 tgcgcctcgg tcagccggcc ggcctcatcg acctggtgaa cctctctgaa tttttcgccg
     2941 gcccggagac ggcccggctc atcgacgccc gaggcatcgc cgcccagttg gatctgcgcg
     3001 gcctgccctt cgccgacgcg gaggagcggc ggtctctcgt cgaaaaactc atcgccctcc
     3061 aggaatcggc ggagagttcg gtgctggaag gcgtgacgcg gacggcggag tccctgccgg
     3121 aagggcggcc gaaagcccgc gaagcggttt ccgacctcat cgtggaggcg gtgatcgccg
     3181 ccggccggga agaaggccag ctggccgccc agaagaacgc caacgtggtg tccgcgtatc
     3241 gggccagcct gcaaacccgt tttgaaaaac tcctcaacga cctgttggcc atcggggcca
     3301 ccacgggact gcgcgccgag gacgaggtcg tcgcggctct ggcggcggtc tacgaaaagg
     3361 cgttcctgca gggagcccag cgctttgagt atcttgccga cgaacaggtc ctggccccca
     3421 aagaagggga aaccgcctcc aagttggacg tggggatgtt cgacgtggtg ggctccttga
     3481 acgatcccca gaacgccgac accgtggcga agatcgtgac gaaggcgggg ctctaccggt
     3541 ccgatctcgg caagttcgcc gccgccatca tgaccgacgg cctgacggcg accgccgacg
     3601 aggtgcgccg ggacgtcctg gcgcggctcg cccggagcgg gttcagcaag gaggagtgcg
     3661 acaagatcag cgccaacctg ctcatcgtgc ccacgttgaa cgcggtggtc ggcgggaaga
     3721 tcgatttgga catcatgttc aacaccgtac aggaacactt caagggccgc atcggccaga
     3781 tcagcctctt tacctccaac ccggcgcggt tccgtgagcc ctggcgggcc cagatcgcct
     3841 gggtgctcta cctcgtcatg ccgcacggcg tcctgcaggc ctccaagagc atcgaagagg
     3901 ggctccgggc cgtccgcgtc ctcaacaccc aggcctaaaa cccgaccgcc gttccgcctc
     3961 atttttgcgc ctttcccggg cgttggcgtt gtttgacaag cggccgcgtt aaacataaaa
     4021 tcaaaccctc tttttacatc gactatgccc tcacacaacg tcactctcat ccc
//
LOCUS       DAOTFD010000222         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16222_length_4072_cov_4.213841, whole genome shotgun sequence.
ACCESSION   DAOTFD010000222 DAOTFD010000000
VERSION     DAOTFD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16222_length_4072_cov_4.213841"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="P5079_10120"
     CDS             complement(<1..1386)
                     /locus_tag="P5079_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY30374.1"
                     /translation="MKLRTRLSLFTILIMTLVVAGTSTATVFFLRKLLYAEARNNQKI
                     ALNNFRKVCEESLITHDDVLLFNYINSAIKTVPGLVYATFVDTDRQLIIGKTEAFSQI
                     FPDIPQASVLEGASTVGDVNIFHLSSNESVEDIASEVTLEGVKYGVVRVGVLQSVSEA
                     NVSAQTQRIQKIVFMVSVVAFFFGLLAAVWIAAQITKPIHLLAEGAKSLGDGNLDTQI
                     DIKRADEIGLLADEFNIMAVKLKELDQLKDDFVSSVSHELRSPLSAISGYVELLTSKP
                     LEQIVPEKRTKAFNIIQESTTRLTQFINDILDLAKLKSGRVEIRRGPMNLKQAAEEIF
                     GLFAPLFEKKKIQSYVYVDSNVSIVQADEEKLKQVITNLASNAIKFTPEGGAISISVD
                     EDEQNLRVSVSDTGVGIPKEYLGMIFERFKQVPGTREKVGGPKGTGLGLAIAKGIVEA
                     HGGKIWAESEPG"
     gene            complement(1478..2767)
                     /locus_tag="P5079_10125"
     CDS             complement(1478..2767)
                     /locus_tag="P5079_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY30375.1"
                     /translation="MPPIPAPPSTPAGAPAKQEPPKSDAPKPAAAPGTTPPAGTPPKP
                     GTGASPAPTAQKTGASGTAPRPRPAGLRSGAKPGASPFKKKFVVMAAAFIGIAILMWG
                     GVKVYRMISGKISSGKKDKGKEGSQEALAVNVLEVKQDRFQDTLVAVGTIAGGSEIPL
                     RFEVDGMIDYFDFREGDKVRKGDVIARLNQRDAYLKMKRAELELDQYEKLYAIGGVAR
                     GRLEESRLAADLARSDLDKTVLRAARDGIVGDKDAEVGEFVTPNKKIATLVNIETVVV
                     RVGIIEKEIDKIFPGQKVMLTVDTYPGVEFTGRVENISPLVQGTSKTLTVEARLDNEG
                     GLLLPGMFARTRIVIFEEDNTIVVPNDSLEKTQAGFRLFVVTKDNKAEARDVEVGYVS
                     SQFSQITKGLAPGELVVTQKPQDLKAGAPVKVIEVQK"
     gene            complement(3361..>4072)
                     /locus_tag="P5079_10130"
     CDS             complement(3361..>4072)
                     /locus_tag="P5079_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRY30376.1"
                     /translation="TSIINAADGVMVARGDLGVEADYADIPILQHRIIGEAHRQGKPV
                     IVATQVLESMLHSPRPTRAEATDVSHAVREGADAVMLSGETSVGKYPVDAVTVLRRLM
                     ESSEEAFASPIPPHLSETPEEKTEDPRAGALGEAAVRLALLLKAQAIVVLSRSGRTAI
                     TTAKNHPPLPVLALSNNPRVRRRLSLFHGIAPIAAPHSFGPEEAAAAGRFLRSQNWLK
                     PGSPFVWVTANSLRLEHP"
BASE COUNT          670 a         1167 c         1346 g          889 t
ORIGIN      
        1 tcccggctcg ctttccgccc agatcttgcc tccgtgcgcc tcgacgattc ctttggcgat
       61 ggcgaggccc agtcccgtcc ccttgggccc gccgaccttt tcgcgcgttc cggggacctg
      121 tttgaatcgt tcgaagatca tgcccagata ttccttcggg atgccgacgc ccgtgtcgct
      181 cacgctgacg cggaggttct gttcgtcctc gtccaccgag atgctgatgg cgccgccttc
      241 cggggtgaac ttgatggcgt tggacgccag gttggtgatc acctgtttga gtttctcctc
      301 gtcggcttga acgatggaca cgttggagtc cacatacacg taagactgga tcttcttctt
      361 ctcgaagagc ggagcgaaga gtccgaatat ctcctcggca gcctgtttca ggttcatggg
      421 gccgcgccgg atctccaccc ggcccgactt gagtttggcc aaatcgagaa tgtcgttgat
      481 gaactgggtg aggcgggtgg tgctctcctg gatgatgttg aacgccttgg tccgtttctc
      541 ggggacgatc tgttccagcg gcttgctggt gagcagttcc acgtagccgg agatggcgga
      601 gagcggcgag cgcagctcgt gcgaaacgct ggaaacgaag tcgtccttca gctggtccag
      661 ttccttcagt ttcaccgcca tgatgttgaa ctcgtcggcc aggaggccga tctcgtcggc
      721 gcgtttgatg tcgatctggg tgtcgaggtt gccgtcgccc agcgacttgg ctccttcggc
      781 gaggaggtgg atgggtttgg tgatctgggc cgcgatccag acggccgcca acaggccgaa
      841 gaagaacgcc acgacggaga ccatgaacac gatcttttgg atgcgttggg tctgggcgct
      901 gacgttggcc tcgctcacgc tctggagaac gcccacccgc accacgccgt atttgacccc
      961 ctccagcgtc acctcgctgg cgatgtcctc gacgctttcg ttggaggaca gatggaagat
     1021 gttgacgtcg ccgaccgtcg aggcgccttc gaggacggag gcctgcggga tgtccgggaa
     1081 aatttgggag aaagcctcgg tttttccgat gatgagctgg cggtcggtgt ccacgaaggt
     1141 cgcgtagacc aggccgggca ccgtttttat cgccgaattg atgtagttga acaagagaac
     1201 gtcgtcgtgc gtgatgaggg actcctcgca cactttgcgg aagttgttga gggcgatctt
     1261 ctggttgttc cgcgcttcgg cgtagaggag tttgcgaagg aagaagacgg tggcggtgct
     1321 ggtcccggcg accaccagcg tcatgatcag aatggtgaag aggctcaggc gggtgcggag
     1381 tttcatcgcg cggacctttc cggggggatg gggcggacaa gccgctgttc tctggaaatc
     1441 attctcgcca ttatataaca atttcggctt cgtcggatca tttttggacc tcgatgacct
     1501 tcaccggggc gccggctttc aggtcctggg gtttctgcgt caccaccagt tcgccggggg
     1561 ccagcccttt ggtgatctgg gagaactggc tggagacgta acccacctcc acgtcgcgcg
     1621 cctcggcttt gttgtccttg gtgaccacga aaaggcggaa ccccgcctgg gttttttcca
     1681 gggaatcgtt ggggacgacg atggtgttgt cctcttcgaa gatcacgatg cgggtgcggg
     1741 cgaacatgcc gggcaggagg agtccgcctt cgttgtccag ccgcgcctcc acggtgagcg
     1801 ttttgctggt gccttgcacc aggggcgaga tgttttccac gcggccggtg aattccacgc
     1861 cgggataggt gtccaccgtg agcatcacct tctgcccggg gaatatcttg tcgatctctt
     1921 tttcgatgat gccgacccgc accaccacgg tttcgatgtt caccagggtg gcgatctttt
     1981 tgttgggcgt gacgaactcg ccgacctcgg cgtctttgtc tccgacgatg ccgtcccgcg
     2041 cggcgcgcag gacggtcttg tccaggtcgg accgcgccaa gtccgccgcc aggcgcgact
     2101 cttccaggcg gccgcgcgcc accccgccga tggcgtaaag tttttcgtac tgatccaact
     2161 ccagttcggc ccgtttcatc ttgaggtagg cgtcgcgctg attgagacgc gcgatgacgt
     2221 cccccttgcg gactttgtcg ccttcccgga aatcaaaata atcgatcatg ccgtccactt
     2281 cgaaacgcag agggatctcc gatccgccgg cgatggtccc gaccgccacg agcgtgtcct
     2341 ggaaacggtc ctgtttcacc tccagaacgt tcaccgcgag ggcttcctgg ctgccttcct
     2401 tgcccttgtc ttttttgccg gaggagattt ttcccgagat catccggtag actttcaccc
     2461 ctccccacat gaggatggcg atgccgatga acgccgccgc catgacgacg aattttttct
     2521 tgaagggcga ggctcccggt ttggcgccgg aacggagtcc ggccggtctc ggccgggggg
     2581 cggttccgga ggccccggtt ttttgggccg tgggggcggg gctcgcgccg gtccccggtt
     2641 tcgggggagt gccggcgggc ggcgtggtcc cgggggcggc cgccggtttt ggggcgtcgg
     2701 atttgggagg ttcctgtttg gcgggcgccc cggccggcgt ggagggcggt gccgggatcg
     2761 gcggcaatcc cgccggggtg ccgccgggcg ccggagaaac aggttttcct tccgccggga
     2821 gagtgtttcc tggggccgaa ggggcgggcg aagagccggg attgtttgga gaaacgggtt
     2881 tatcttccac gaagttgtcg gatcacgctt gtttccgaag attttgcatc tggaatatat
     2941 agcaaaaatc gccggaagcg taaataacgg atattgtcac aaaggtgact attttttgaa
     3001 aacaagtaag ttgatctcag ttccccactg ttttcgactt cgccgcgctc cgctcgaaca
     3061 atccaatctt cgagcgaccg tcccaacctc aaacggcaga ggggcggccc cgcaacacgt
     3121 ccgctgcgac ggcgggatct gtaacacagg cccctcgttg ctgctgggca acgtcccggt
     3181 ttgtcggacg gggcacgtgt tcgggggttg gggccgaagg ggtcgagaag ttacttgtct
     3241 tcaagggacg ctcaccgtca caagaagacg gatttgtgtt gtggaacggc cggcgacggg
     3301 gtgaattagc gcccggcctc tccgtgcccg aggaggagaa aacgtccttc ctgaaaattt
     3361 ttagggatgt tccaggcgaa gggaattggc cgttacccag acaaacgggg agccgggttt
     3421 cagccaattt tgggatcgca ggaaccgtcc ggcggccgcg gcctcttccg gtccgaacga
     3481 gtgcggcgcg gcgatggggg cgatgccgtg aaacaaagag agtcggcgcc gcacccgggg
     3541 attgttggac aacgccaaaa cggggagggg cggatggttt ttggccgtgg tgatggccgt
     3601 tcgacccgac cggctgagca ccacgatggc ctgtgctttg agcagaaggg ccaaccggac
     3661 ggccgcttcg cccagagccc cggcccgcgg gtcctcggtt ttttcttccg gtgtttccga
     3721 gaggtgggga gggatcgggg aggcgaacgc ttcttcggat gattccatga ggcggcgcag
     3781 gaccgtcacg gcgtcgacgg gatattttcc gacggacgtt tctccggaga gcatgacggc
     3841 gtccgccccc tcccggacgg cgtgggacac gtccgtggct tccgcccgtg tggggcgagg
     3901 ggagtggagc atggattcca aaacctgggt ggccacgatg acgggtttgc cctgccggtg
     3961 cgcctctccg atgatgcggt gttgcaggat cgggatgtcg gcgtagtccg cctccacccc
     4021 caaatcccct ctcgccacca tcaccccgtc cgcggcgttg atgatggagg tc
//
LOCUS       DAOTFD010000223         4070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16234_length_4070_cov_3.993524, whole genome shotgun sequence.
ACCESSION   DAOTFD010000223 DAOTFD010000000
VERSION     DAOTFD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16234_length_4070_cov_3.993524"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1673)
                     /gene="lysS"
                     /locus_tag="P5079_10135"
     CDS             complement(69..1673)
                     /gene="lysS"
                     /locus_tag="P5079_10135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY30377.1"
                     /translation="MEPTQPVSSLDELIKQRREKLKKARENGVNPFPTTFAVTHTANA
                     VRLLCEPLSAEGQVSEEVAVAGRLMTRRDMGKAAFAHLQDRSGKIQLYLRKDELGDAA
                     YDLFKNQVDLGDILGVTGMPFKTKTGEPSIRVKTCHLLSKALRPPPEKFHGMTDVEVR
                     YRRREVDLFSNEEARTRFWLRRKIVTSLREVLDKDGFIEVETPMMQPVPGGAAAKPFI
                     THHETLDMDLYMRVAPELYLKRLLVGGLERVYEIGRAFRNEGIDTRHNPEFTILEAYQ
                     AYTDFQGMRFLTEKIIDAAAKTVIEKTWPTPEKGSLRVDVGPMSLGPELSEGNKILFP
                     YRGRTFSLYASSYPGGHFPWLSMGELFKEHLGLDYRALCRDNNWKEAAKKAGVSIEGN
                     PPDHKFFDALFDQKILPHLPAVAFVHGYPAVFSPLAKCAPESPDVAERFELFICGDEV
                     ANAFSEQNDPDVQRKHFIEQARQRQAGDEEAMPADEEFVIALEHGMPPAGGLGIGVDR
                     LTMILTGTESIREVILFPLLRPEKNA"
     gene            complement(1694..2065)
                     /locus_tag="P5079_10140"
     CDS             complement(1694..2065)
                     /locus_tag="P5079_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30378.1"
                     /translation="MAKILVVDDDKDIVDLICFLVQKDGHTTLKAGDGQACLDQVARE
                     KPDMIVLDVMMPVMDGYTVVTQLASNDETRAIPILILTAKGQMREMFQMASNVADYLE
                     KPFDPDKLRAQVTKILAGRPR"
     gene            complement(2136..3305)
                     /locus_tag="P5079_10145"
     CDS             complement(2136..3305)
                     /locus_tag="P5079_10145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30379.1"
                     /translation="MPENSAPAPTSSFDPTLDLGEPDAPTAGKAPETATPVSITLGTH
                     NACNAKCIFCMEGRFGGFGLDFYKEFFEKKLGPYLAEAEQITFTGFGETLLLPDIAPF
                     LDHLNRTLPNTKKIFTTNGTALTPGVVEKLRTGKYLIQISLHASRADIHEQLTLLPGE
                     FSVILENIRALRKAAGENLEITLVNVVNTLNVDDLPEFVKLGWDLGVHRVRAYYLTAF
                     KPEHLRLSCFFDQARANRALQAARELHQSLASRDSSRRFQLFLPASFGSSQDASTSPV
                     QCRLPWQHIFVDIYGSVLPCCQWGEVVDNLNKTSAEAIWNGPFYKTLRASMSSGDLLP
                     ACQKCPRFKPANVNDLLAHITSRPDQKKAILAALSPKDTGDVGADVSGKSLSLPC"
     gene            complement(3311..>4070)
                     /gene="prfB"
                     /locus_tag="P5079_10150"
     CDS             complement(3311..>4070)
                     /gene="prfB"
                     /locus_tag="P5079_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRY30380.1"
                     /translation="AIVSLHAGAGGTAACDWVEMLLRMYSRWSEAKGFPTEIVDILPG
                     EEAGIKRVTFLVKGAFAYGLLQSEIGVHRLVRISPFDSNARRHTSFAACDILPEVDDT
                     IEITVNESDLRIDTYRASGHGGQHVNRTDSAVRITHLPTNIVVQCQNERSQFKNKAMC
                     MKMLKAKMYDLELEKQRAVVEKHYDAKGDIAWGNQIRSYVFMPYQMVKDLRSGQETSQ
                     ISAVMDGALDEFMHAYLEWKLSGEKPRRSSADAG"
BASE COUNT          736 a         1121 c         1322 g          891 t
ORIGIN      
        1 ttggcgagga agagttcaaa agacatggag gatggggatg atttcttggg ccggccgaca
       61 aaaacgcgtt acgcgttctt ttccggacgc aggagaggga agaggatcac ttcccggatg
      121 gattccgtgc cggtgaggat catggtgaga cggtccaccc cgatgcccag cccgccggcc
      181 ggcggcatgc cgtgttccag cgcgatgacg aactcctcgt cggccggcat ggcctcctcg
      241 tcgcccgcct gacgctggcg tgcctgttcg atgaaatgtt ttctctgcac gtcggggtcg
      301 ttttgttccg agaaggcgtt ggctacttcg tctccgcaga taaacagttc gaaccgttcg
      361 gcgacgtccg gggattcggg agcacacttc gccagtggcg agaaaaccgc cggatagccg
      421 tgcacaaacg ctaccgccgg caggtgcggc aggattttct ggtcgaacag ggcgtcgaaa
      481 aatttatggt cgggcgggtt gccttcgatg gacaccccgg ctttttttgc agcctccttc
      541 cagttattgt cccggcagag agcccggtaa tctagtccga gatgttcctt gaacaattct
      601 cccatggaga gccaaggaaa atgcccgccc ggataagaac ttgcatataa cgaaaaagtg
      661 cgtccgcggt agggaaagag gattttattc ccttcgctta attcgggccc aagagacatc
      721 ggtcccacat cgacgcgtag acttcctttt tcgggtgtcg gccaagtctt ttcgataaca
      781 gttttcgccg ccgcgtcgat gattttttcc gtcaaaaaac gcatcccctg gaaatcggtg
      841 taggcctggt aggcttccag aatggtgaat tccgggttgt ggcgcgtatc aataccttca
      901 ttacggaaag cccgaccaat ttcgtagacg cgttccaacc cacccaccaa aagacgtttt
      961 agatagagtt caggagccac gcgcatgtag aggtccatgt ccagcgtttc atggtgtgtg
     1021 atgaacggtt tggcggccgc tccaccgggc accggttgca tcatgggcgt ctccacttcg
     1081 atgaagccgt ccttgtccaa aacttcgcga agagaggtga cgatttttcg cctcagccaa
     1141 aaacgcgtgc gtgcctcctc gttcgaaaat aggtccactt cccgtcggcg gtagcggact
     1201 tccacgtcgg tcatgccgtg gaacttctcc ggcggcgggc gcagcgcctt gctcagaaga
     1261 tgacacgtct tgacgcggat ggagggttcc cccgtcttcg tcttgaaggg catgcccgtc
     1321 acgcccagga tgtcgccgag gtccacctgg tttttgaaca ggtcgtaggc ggcgtcgccc
     1381 agttcgtctt tccgcaggta gagttggatt tttccggacc ggtcctgcag gtgggcgaag
     1441 gcggctttgc ccatgtcgcg gcgggtcatc agccgcccgg ccacggcgac ctcttccgaa
     1501 acctgtccct ccgcgctcag cggctcgcac agcaggcgga ccgcgttcgc cgtgtgcgtg
     1561 acggcaaacg tcgtggggaa cgggttgacg ccgttctccc gcgccttttt cagtttctcg
     1621 cggcgctgtt tgatcagttc atccaatgag gagacgggtt gcgtcggttc catggcgggt
     1681 cggccgggcc ggttcagcgg ggccggccgg cgagtatttt ggtcacttgc gcccggagtt
     1741 tgtccgggtc aaaaggtttt tcaaggtagt ccgccacgtt ggaggccatc tggaacatct
     1801 cccgcatctg gcctttggcg gtgaggatga ggatggggat ggcgcgcgtc tcgtcgttgg
     1861 aggcgagctg ggtcaccacc gtgtagccgt ccatcacggg catcatcacg tcgagaacga
     1921 tcatgtccgg tttttcccgg gccacctggt cgagacacgc ctggccgtcc ccggccttca
     1981 gggtcgtgtg gccgtctttt tggacgagga aacagatcag gtccacgatg tctttgtcgt
     2041 catccaccac gaggattttc gccacggtgc ctcccgagtg taaaaaagga ttctataaag
     2101 ctttgcgatt cattgtcaaa aaacgtttga acgattcagc agggcaaaga gagcgacttt
     2161 cccgacacgt ctgcgcccac gtctccggtg tcttttgggg agagagcggc caaaatggct
     2221 tttttctggt ccggccggct ggtaatgtga gcgagaaggt cgttgacgtt ggccggctta
     2281 aagcgcggac atttttggca ggccggaagc aggtccccgg acgacatgga ggcccgcagc
     2341 gtcttataga acgggccgtt ccagatcgcc tcggccgacg ttttattcaa attgtcgacg
     2401 acttcccccc attggcagca gggcagaacg gagccgtaga tgtccacgaa gatgtgttgc
     2461 caggggaggc ggcattggac gggagaggtc gacgcgtcct gggacgatcc aaaggaggcc
     2521 ggcaggaaaa gctggaaccg tcgggacgag tcccgggaag cgagagattg gtggagttcc
     2581 cgggcggcct gaagggcgcg gttggcccgg gcttggtcga aaaaacagga aaggcggagg
     2641 tgctcgggct tgaaggccgt caggtaatag gcccggacgc ggtgcacccc gaggtcccat
     2701 cccagcttga cgaactcggg caggtcgtcc acgttgagcg tgttgaccac gttgaccagg
     2761 gtgatttcca ggttctcccc ggcggctttc cgaagggcgc ggatgttctc caggatgacg
     2821 ctgaattctc ccgggagaag ggtcagctgt tcgtggatgt ccgcccggga ggcgtggagg
     2881 gagatttgga tgaggtattt cccggttctt agtttttcaa cgacgccggg cgtgagggcg
     2941 gtgccgttgg tggtgaagat tttttttgtg ttcggcagag tccggttgag atggtcgagg
     3001 aacggcgcaa tgtccggcag gaggagcgtt tcgccgaagc ccgtgaaggt gatttgttcc
     3061 gcctcggcaa ggtaaggccc cagttttttt tcgaagaatt ccttgtagaa atcgaggcca
     3121 aacccgccga atcggccctc catgcagaag atgcatttgg cgttgcaggc gttgtgcgtg
     3181 ccgagggtga tggagacggg agtcgccgtc tccggggcct tgccggccgt cggggcgtcg
     3241 ggttctccca ggtcgagggt ggggtcgaaa gaggaggtgg gcgcgggggc ggaattttcc
     3301 ggcacggcgg tcacccggcg tcggcggagg aacgccgcgg tttttcgccg ctgagtttcc
     3361 attccaggta ggcgtgcatg aactcgtcca gcgcgccgtc catcaccgcg gaaatctggg
     3421 aggtttcctg gccgctgcgg aggtccttca ccatctggta gggcatgaag acgtaggagc
     3481 ggatctggtt cccccaggcg atgtcgcctt tggcgtcgta gtgtttttcg acgacagcac
     3541 gctgtttttc cagttccagg tcatacatct tcgccttgag catcttcatg cacatggcct
     3601 tgttcttgaa ctgggaccgc tcgttctggc attgcacgac gatgttggtg gggagatgcg
     3661 tgatgcgcac cgccgagtcc gtccggttga cgtgctgccc gccgtggccg ctggcgcggt
     3721 aggtgtcgat gcggaggtcc gactcgttga cggtgatctc gatggtatcg tccacctccg
     3781 gcaggatgtc gcaagccgcg aaggaggtgt gccgccgcgc gttgctgtcg aacggcgata
     3841 tccgcaccag gcggtgcacg ccgatctccg actgcagcag gccgtaggcg aaagcgcctt
     3901 tgacaaggaa ggtgacccgt ttgatgccgg cctcttcccc cggaaggatg tccacgattt
     3961 ccgtcggaaa acctttggct tccgaccagc ggctgtacat ccgcaaaagc atctccaccc
     4021 agtcgcaggc tgccgtgccg ccggcgccgg cgtggaggga cacgatggcg
//
LOCUS       DAOTFD010000224         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16243_length_4068_cov_4.215549, whole genome shotgun sequence.
ACCESSION   DAOTFD010000224 DAOTFD010000000
VERSION     DAOTFD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16243_length_4068_cov_4.215549"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1661)
                     /locus_tag="P5079_10155"
     CDS             complement(36..1661)
                     /locus_tag="P5079_10155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30381.1"
                     /translation="MPSPPISSRTLNLRTPLLLSALIAFYAIQHIVWLRHFGARAPGE
                     DLGQIELMIPHYSALLDGRWGDFMALLRENYSYSPLYYLGSIVSVFLFGKSIVSLLLP
                     NLLYFSLAVVFIQFLGERFQNKTLGLWSAALFSLTPAVYLYSRSYCLEFGTMGLLPLT
                     LWLLLKSEGFSRPLPCIGFGMAAAAASLMKDTHIALAFIAGPLLYVLYLAWKKIFVGN
                     KRVLFNIFLAAAVFVGLLAPRYWNLDALRWRDPMEEIAGWVDVSVMTWGFIHRQLSWP
                     LFLLFIVGGLALLKNRNREPLFVILPWILVPWTILTFMPHWKLTRHAIPYLPAVALAM
                     GYGLLSIGNRMVRDILGTAILLVAAVQFYQFSSPIPPKNRLVSKNFVIDNKVVSMSEH
                     RWKRNPIAVLEMVDRLKPAGGKLLIASRSHALWGLSTWLYAPRHWTISFIKTGKPPWD
                     MRPNERWLSNFKTCDFLVTSESWDLAHHDLVAKWVQDLQSTNYPWETSLQREETEFWR
                     QYRDVRKTLRFQEIAVFLDEELPDFSVRLYARQ"
     gene            1979..3196
                     /locus_tag="P5079_10160"
     CDS             1979..3196
                     /locus_tag="P5079_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30382.1"
                     /translation="MFEDKIRGEPIRIINLAEQGACLEDQYWKLFRELFLRPPKGRGV
                     LLIYAGINEPAGPDESRVFFAWRLWQRSIVLSRLEYLMRRDSEFLGAGFTDGKYETRL
                     QKIIGLGKKHGLTVIVSTLVGNLRYEPTQDNAGVLEYFGEAYEAERREEFRRALGLYE
                     EARSATKGGVLAFKYFHTARCHERLGEYETAKEFYWKAVDCGNGRRPTRRQNRIIVGL
                     AKKNGVLLVDAARRFEERAPHGIPGYDLFMDAHHPAILGYLLLATGFSEEIGRAFQTA
                     RVWRTPTPADIASFFEFGPEDFFDTYISRVLWFCGEATEQEGRSERMEIARRYLAAAE
                     RIHGENINVYLGHLMICTLEKDSLGIRHWLEKGKFLTENKDFFETNTSAEFRGWFAKR
                     LAEAVVSPAVGRE"
     gene            complement(3267..>4068)
                     /locus_tag="P5079_10165"
     CDS             complement(3267..>4068)
                     /locus_tag="P5079_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY30383.1"
                     /translation="GWVRPSTRDVGGNSGEGRPLPELVESELQQRGFHNVHVINGGVP
                     SHVSGQELATIVYRVVDLKPDLIVMYDGGNDILSPYDDDPRPGYPFNFMVSERNPLLQ
                     NGARYPLFSLMAFKSELLRTLLHPYFVEKFTRRSDFRKQSGYETESWREEIAKNYVRN
                     LVKAQTISRAFGAEFIAFFQPLAFFARPDCETPLGRHALKVREHISREMRRAREIHSL
                     NVVDLSELFKNETDEIFGDPIHIKDASNKAVARTIAAHLSPLIKKIRR"
BASE COUNT          917 a          963 c         1276 g          912 t
ORIGIN      
        1 tatgggtaaa aaggaggggg acgggaaggg ggtgtttatt gccttgcgta aagacgaaca
       61 ctgaaatcgg gcagttcctc gtcgaggaac acggcgattt cttgaaaacg gagggttttc
      121 cgaacgtccc tgtattggcg ccagaattcc gtttcttcgc gttggaggga ggtctcccag
      181 gggtaattgg tggactggag atcttgcacc catttcgcca caagatcgtg atgggctaaa
      241 tcccacgact ccgaggtgac gagaaaatcg caggttttaa aatttgagag ccacctttca
      301 ttcgggcgca tgtcccaggg aggtttgccc gtttttatga aagaaatcgt ccagtggcgc
      361 ggggcataaa gccaggtcga cagtccccat aaagcgtgac ttcgggaggc gatgagcagt
      421 tttccaccag ccggtttcag gcggtcgacc atttccagaa cggcgatcgg atttctcttc
      481 cagcggtgtt cgctcatcga aacgactttg ttgtcgatca caaaattctt ggaaacaagg
      541 cggtttttcg gagggatggg ggaggagaat tgatagaact gcacggccgc cacaaggaga
      601 atggctgttc cgaggatatc tcggaccatt cggttcccta tcgacaggag gccatatccc
      661 atggccaggg cgaccgcggg cagatagggg atcgcgtgcc gggtcagttt ccagtggggc
      721 atgaatgtca gtatcgtcca gggaaccagt atccacggga ggatgacgaa gagcggctcc
      781 ctgtttcgat tttttagcag ggcgagccca cccactataa agaggagaaa aagcggccag
      841 gacagctgcc gatggatgaa tccccacgtc atgacggaga cgtcgaccca tccggcgatt
      901 tcttccattg ggtctctcca gcggagtgcg tctaggttcc agtagcgcgg tgcaagtagg
      961 ccgacaaaaa cggcggcggc cagaaatata ttgaagagaa cgcgtttatt gccgacgaag
     1021 atttttttcc acgccagata aaggacgtag agcagcggtc ctgcgatgaa ggcaagggct
     1081 atgtgggtgt ctttcatcag gctggccgct gctgcggcca ttccgaaacc gatacatggc
     1141 aggggccggg aaaatccttc gcttttcagg agaagccaga gggttagggg gagcagtccc
     1201 atcgttccga attccaggca atacgatcgg gaataaagat agacagcggg ggtcaatgaa
     1261 aaaagagccg ccgaccaaag gcccagagtc ttgttttgaa aacgttcgcc gagaaattgg
     1321 atgaaaacaa cagccagtga aaaatacaat aggttgggca agaggaggct gacgatgctt
     1381 ttcccaaaga gaaaaacgct gacgatggaa ccgaggtaat acagcggcga atagctgtaa
     1441 ttttcccgca ggagcgccat aaagtccccc cagcgaccat ccaaaagcgc cgaataatgg
     1501 ggaatcatca actcgatttg tcccagatct tctccgggcg cgcgcgctcc gaaatggcgc
     1561 aaccacacga tgtgctgtat cgcgtaaaac gcgatgaggg cactcaggag gagaggggtt
     1621 cgtagattca acgtccggga cgagatcggg ggtgatggca tgtgaaattc tccctcagcc
     1681 aaatgcattt tgataccatg tgaaaaatta caccattacg gataatatga ttttccgaca
     1741 gagaaaacga agatttttaa tgaatttcgc tgcgagactc ctgctcttgg ggggcggcgc
     1801 ggtggtggcg ctcttggtgg cggaagcggg agtgcctctg gcgggttctg tttggggggg
     1861 cattttttcg cgtcttgcaa agggggatgg gggatttctc ttgtacgctg tgggtgagtc
     1921 aacggtggtc ggagaacctt tcgcgccgaa aatatccttt ccgaagataa ccgcgcgcat
     1981 gttcgaagac aaaataaggg gggagcccat ccggataatc aatctcgccg aacagggggc
     2041 gtgtctggag gaccaatact ggaaactttt tcgggaattg ttccttcgcc ctcccaaggg
     2101 gcgaggggtg ttgttgattt atgcgggcat taacgagccg gcgggccccg acgagtcccg
     2161 cgtttttttc gcgtggcgat tatggcaaag atccatcgtg ttgtcgcgtt tggaatactt
     2221 gatgaggcgg gacagcgaat ttctcggggc cggcttcacg gacgggaaat acgagacccg
     2281 tttacagaaa attattgggc tgggaaaaaa gcatggctta accgtgatcg tgtcgaccct
     2341 cgtcggcaat ctgcgctatg aaccaacgca ggacaacgcc ggggttttgg agtattttgg
     2401 agaggcgtat gaggccgaga ggcgggagga attcagaagg gcgttggggc tttatgagga
     2461 ggcgcgctct gcgacaaagg gcggcgtctt ggcgtttaaa tattttcata cggcgcgctg
     2521 ccatgagcga ttgggcgagt atgagacggc aaaggaattc tattggaaag cggtcgattg
     2581 cggcaacggt cgtcggccga cgcgccggca aaaccggatc atcgtcggat tggccaagaa
     2641 gaacggagtt ctccttgtgg acgccgctcg tcgtttcgag gagcgtgctc cccatggcat
     2701 tccaggatac gatcttttca tggacgcgca tcaccccgcg atcctcggct atcttttgct
     2761 ggccaccggt ttttcggaag agatagggcg ggcctttcaa acggcgcggg tttggaggac
     2821 cccgactcca gcggatatcg cgtctttttt cgagttcggc ccggaggatt ttttcgacac
     2881 atatatctcg agggtgttgt ggttctgcgg ggaagcgact gagcaggagg ggcggtctga
     2941 acggatggaa atcgccaggc gatatctggc ggcggcggag cggattcacg gggaaaatat
     3001 caacgtttat ctgggacacc tgatgatctg cactcttgaa aaagacagtc tcggtatccg
     3061 tcattggctt gaaaagggga aattcctgac ggaaaacaaa gatttctttg agacgaacac
     3121 ttcggctgag ttccgtggct ggttcgcgaa gcgcttggcg gaggcggtgg tttctcccgc
     3181 cgttggccgg gaatagccgc cgctttcgac ggctattggg gcgacgactg cgaggtggcg
     3241 ctgggggcgg tttgtccgct gcagggttaa cggcggattt tttttatcag cggggaaagg
     3301 tgggctgcga ttgttcgggc cacggcttta ttcgatgcgt ccttgatgtg gatgggatct
     3361 ccgaaaatct cgtcggtctc atttttgaac agttcactca agtcgacgac gttcagggaa
     3421 tggatttcgc gcgctcttcg catctcgcgg gagatatgct cacgcacctt gagcgcatgg
     3481 cggccaaggg gagtttcgca gtctggtcgg gcgaagaaag ccagcggctg gaaaaaggcg
     3541 atgaattcgg cgccgaacgc cctggaaatg gtctgggctt ttaccagatt gcgtacatag
     3601 ttcttcgcga tttcttcccg ccacgattcg gtctcgtaac cgctttgttt cctgaaatcg
     3661 gaccgccgcg tgaatttttc gacaaaatag gggtgcagga gcgtccgtaa tagctcgctt
     3721 ttgaacgcca tgagggagaa cagggggtat cgggcgccgt tctggaggag cggatttctt
     3781 tcggaaacca tgaagttgaa gggatatccg ggtcgcggat cgtcgtcata cggcgacagg
     3841 atgtcgtttc ccccgtcgta cataacgatc aggtcaggtt ttaagtccac gacccggtag
     3901 acgatggttg ccagttcttg accgctcacg tgggagggca cgccgccgtt aatcacgtgc
     3961 acgttatgga atccgcgttg ttgcagttcc gattcgacca gttccgggag cggccgcccc
     4021 tcgcccgagt tcccgcccac gtcgcgcgtg gaagggcgaa cccatccc
//
LOCUS       DAOTFD010000225         4046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16357_length_4046_cov_4.099975, whole genome shotgun sequence.
ACCESSION   DAOTFD010000225 DAOTFD010000000
VERSION     DAOTFD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16357_length_4046_cov_4.099975"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="P5079_10170"
     CDS             <1..534
                     /locus_tag="P5079_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY30384.1"
                     /translation="IDLPFAVPTLVTGVMLVALFGPQAALGAWLNDAWGWQLMFAPPG
                     IILALMFVSLPMVVRSVQGLLLEMDRDQEEAAVSLGATGPTVFWRVLFPTILPGMAAG
                     ALLSFSRALGEFGAVAVVAGNIPFYTQTAAVYVLGEVEGGRRASAGAMSVVLVTVSFV
                     LVLAVEAWQNRRMKNAV"
     gene            524..1399
                     /locus_tag="P5079_10175"
     CDS             524..1399
                     /locus_tag="P5079_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HRY30385.1"
                     /translation="MPFERGAAPLFRGERWLNRTLIGVVLAYVGILLAAPCAALARGA
                     FAGGGAVLWRELARPDVLSAFRLTLALALGAVALNTVFGVLTAWVLVRHSFPGRRMLN
                     ALVDMPFAVSPVVAGYMLILLFGRGGWLAGWVEWSGFRVVFSWPGMLLATVFVTMPFV
                     VREVMPVLREFGAEPEEAAATLGAGPWRIFWRVTLPSVRWGLLYGVALTLARALGEFG
                     AVYVVSGAVQGRTETAPLFVFRALDERLTVAAYGVSLVLALLSFSLITALGAYQKREE
                     EKISPRGPGAEREAA"
     gene            1396..2436
                     /gene="cysA"
                     /locus_tag="P5079_10180"
     CDS             1396..2436
                     /gene="cysA"
                     /locus_tag="P5079_10180"
                     /EC_number="7.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HRY30386.1"
                     /translation="MSIEARNLSKSFGRFKAVDDVSFRLDEGVLAALLGPSGSGKSTL
                     LRMISGLEAPDRGEIFIMGQDATRRAARERNVGFVFQHYALFKHMTVWQNIAFGLEAR
                     RRPKEEIRARVTDLLRLVQMEGMEKRYPSQLSGGQRQRVALARALAPQPRVLLLDEPF
                     GALDAKVREELRGWLRQLHEHSRVTTLFVTHDQREALEIADKILVLDKGRLEQQGTPR
                     EIFDKPATPFVAQFVGENNFIDAEVTEPELVCWGPFRFEVGESLAVGRKVRLFFRPYD
                     VYVSNKLVTLQVRANIVTSRFRGPVMELVLDVGGDKRVTAQVPKGIVEARGFSAGRDV
                     FVGITAYHVFPQ"
     gene            2458..3081
                     /gene="cysC"
                     /locus_tag="P5079_10185"
     CDS             2458..3081
                     /gene="cysC"
                     /locus_tag="P5079_10185"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRY30387.1"
                     /translation="MEDKEKRDIVWHHGKLTPAAREKSLGGRGAVVWFTGLPCSGKSS
                     LAREVEAALVEGGVNAAVLDGDNIRHGLNRDLGFSPADRAENIRRIGEVAKLFVQNNV
                     VALTAFISPYRSDRAAARALVAPGEFFEVFCDAPLAVCEQRDTKGQYAKARAGRIPEF
                     TGVSAPYEPPDKPELTLKTGEETLEQSARRVLDLLKKAGLGRRLRRD"
     gene            3098..3910
                     /locus_tag="P5079_10190"
     CDS             3098..3910
                     /locus_tag="P5079_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY30388.1"
                     /translation="MIEIDGFLPEAKELAVRAGARLRELFNTPLVRERKADRSLVTNA
                     DRESDELLRSGLRKAFPSHAVLSEEAVLEGPVSDYVWLVDPLDGTKAFAEGRPGFCVM
                     LGLLWKEMPVVGVVYEPLEDHLTWAAKGGGCFHSQGGEARRVRVSDRSDLSRMPVVTS
                     TGLTPAMEEHLRKNFSGPLLEPINSVGVKIGYLVRQAADFYVNHHLVHMWDTCAPGVI
                     LEEAGGVMTLWDGTPLTYTVSGPSRHPGPTAASNGRRHPEFLKILSTFPSRP"
BASE COUNT          583 a         1210 c         1549 g          704 t
ORIGIN      
        1 atcgacctgc ctttcgcggt gcccaccctg gtgacgggcg tgatgctggt ggcgctcttc
       61 ggcccgcagg cggcgctggg cgcctggctg aacgacgcgt ggggctggca gctgatgttc
      121 gctccgccgg gcatcatcct ggcgctgatg ttcgtgagcc tgcccatggt ggtgcggtcg
      181 gtgcagggcc ttctgctgga gatggaccgc gaccaggagg aggccgcggt gagcctgggg
      241 gcgaccggcc cgacggtttt ttggagggtg ctgttcccga cgatcctgcc gggcatggcg
      301 gcgggcgcgc tgttgagttt ttcgcgggcg ctgggggagt tcggcgcggt ggcggtggtg
      361 gcgggcaaca tcccttttta cacgcagacc gcggcggtgt acgtgctggg cgaggtggag
      421 ggcggccgcc gggcctccgc cggggcgatg tcggtggtgt tggtgacggt gtcgttcgtg
      481 ttggtgctgg ccgtggaggc ctggcagaac cggaggatga aaaatgccgt ttgaacgcgg
      541 cgccgcgccg ctgttccggg gagaacggtg gctgaaccgg acgctgatcg gcgtggtgct
      601 ggcctacgtg gggattttgc tggcggcgcc ctgcgcggcg ctggcgcgcg gcgcgttcgc
      661 cggcggcggg gccgttctgt ggcgcgaact ggcccggccg gacgtgctgt ccgcgttccg
      721 cttgacgctg gcgctggccc tgggcgcggt ggcgctcaac accgttttcg gcgtgctcac
      781 cgcgtgggtg ctggtgaggc attcgttccc cggcaggaga atgttgaacg ccctggtgga
      841 catgccgttt gcggtgtcac cggtggtggc ggggtacatg ctcatcctgc tgttcggccg
      901 cggcggctgg ctggcgggct gggtggagtg gagcgggttt cgggtcgttt tttcctggcc
      961 gggcatgttg ctggccacgg tgttcgtgac gatgccgttc gtggtgcggg aagtgatgcc
     1021 ggtgctgcgg gagttcggcg cggaaccgga agaggcggcg gccacgctgg gcgccggccc
     1081 gtggcggatc ttttggcggg tgacgctgcc gagcgtgcgg tggggcctgc tctacggcgt
     1141 ggcgctgacg ctggcgcggg cgctgggcga gttcggtgcg gtgtacgtgg tgagcggcgc
     1201 ggtgcagggc cgcacggaaa cggcgccgct gttcgtgttc cgcgcgctgg acgaacggct
     1261 gaccgtggcc gcctacgggg tctccctggt gttggcgctg ctgtcgtttt cgctgatcac
     1321 cgcgttgggg gcgtatcaga aacgtgagga agaaaagatc tctccccggg gccccggggc
     1381 ggaacgggag gcggcgtgag catcgaggcg cggaacctgt cgaagagttt cggccgtttc
     1441 aaggccgtgg acgacgtgag tttccgcctg gacgaaggcg tgctggcggc gctgttggga
     1501 cccagcggga gcggcaagag cacgctgttg cgcatgatct ccgggcttga ggcgccggac
     1561 cgcggggaga tcttcatcat ggggcaggac gccacccgcc gcgccgcccg ggaacgcaac
     1621 gtggggtttg tgttccagca ttacgcgctc ttcaaacata tgacggtgtg gcagaacatc
     1681 gcgttcggat tggaggcccg ccgccggccg aaagaggaga tccgcgcccg cgtgacggac
     1741 ctgctgcgcc tggtgcagat ggaggggatg gagaaacggt atccgtcgca gttgtccggc
     1801 ggccagcgcc agcgggtggc gctggcgcgc gccctggcgc cgcagccgag ggtgctcctg
     1861 ctcgacgagc cgttcggcgc gttggacgcc aaggtgcgcg aggaactgcg cggctggctg
     1921 cggcagctgc acgaacacag ccgcgtgacc acgctgttcg tcacccacga ccagcgcgag
     1981 gcgctggaga tcgccgacaa gatcctcgtg ctcgacaagg gccgcctgga acagcagggc
     2041 accccccgcg agatcttcga caagcccgcc acccctttcg tggcccagtt cgtgggagag
     2101 aacaatttca tcgacgccga ggtgaccgaa ccggaactgg tctgctgggg cccgttccgt
     2161 ttcgaggtgg gcgagtcgct ggccgtgggc cggaaggtgc gcctcttctt ccggccgtac
     2221 gacgtctacg tgagcaacaa gttggtgacc ctgcaggtgc gggcgaacat cgtgacgtcg
     2281 cgcttccgcg ggcccgtgat ggaactggtt ttggacgtgg gcggggacaa acgcgtgacc
     2341 gcgcaggtgc ccaaaggcat cgtggaggcg cgggggtttt ccgcggggcg ggacgtcttt
     2401 gtgggtatca ccgcctatca cgtttttccg cagtgacctt gggacgggga ggacggcgtg
     2461 gaggataaag agaagcggga catcgtatgg caccacggca aactgacgcc ggcggcgcgt
     2521 gagaaaagcc tggggggccg cggcgcggtg gtgtggttca ccggcctgcc gtgttccggg
     2581 aaatcctccc tggcgcggga ggtggaggcg gccctggtgg agggcggcgt gaacgccgcc
     2641 gtgctggacg gcgacaacat ccgccacggg ttgaaccgcg acctggggtt ctctccggcc
     2701 gaccgggccg agaacatccg gcgcatcggc gaggtggcga aacttttcgt gcagaacaac
     2761 gtggtggcgc tgaccgcgtt catctcgccc taccggtcgg accgcgccgc ggcgcgggcg
     2821 ctcgtggcac ccggcgaatt tttcgaggtc ttctgcgacg cccccctggc ggtgtgcgaa
     2881 caacgcgaca ccaaaggcca atacgccaag gcccgcgccg gccggatccc cgagttcacc
     2941 ggcgtgtccg cgccctacga gccgccggac aagccggaac tgacgctgaa gaccggcgag
     3001 gagacgctgg aacagagcgc ccgccgcgtg ctcgaccttc tgaaaaaggc cggcctgggc
     3061 cgccgcttgc ggcgggacta ggcgttcgcg cggcgcgatg atcgagatcg acgggttcct
     3121 gccggaggcg aaagaactgg cggtgcgcgc cggcgcccgc ctgcgggaac tgttcaacac
     3181 gccgctggtg cgcgaacgca aggcggaccg gtccctcgtc accaacgccg accgcgaatc
     3241 ggacgagctt ctacgcagcg ggttgcgcaa ggcgttccct tcgcacgccg tgttgagcga
     3301 ggaggcggtg ttggaagggc cggtgtcgga ttacgtgtgg ctggtggatc ccctggacgg
     3361 caccaaggcg ttcgcggaag ggcgtcccgg gttctgcgtg atgctggggc tgctgtggaa
     3421 ggaaatgccg gtggtggggg tggtgtacga gccgctggag gaccatctca cctgggccgc
     3481 caaaggaggc ggctgtttcc attcgcaggg cggcgaggcc cggcgcgtgc gcgtctccga
     3541 ccggtcggac ctttcgcgga tgccggtggt gacctccacc gggctgacgc cggcgatgga
     3601 ggaacatctc cggaaaaatt tttcaggacc gctgctcgaa ccgatcaaca gcgtgggcgt
     3661 gaagatcggc tatctggtgc ggcaggcggc ggatttttac gtgaaccacc acctggtcca
     3721 catgtgggac acctgcgcgc cgggggtgat cctcgaagag gccggcggcg tgatgacgtt
     3781 gtgggacggc acgccgctca cctacacggt gagcggccct tcccggcatc cgggccccac
     3841 cgcggccagc aacggccgcc ggcatccgga attcctcaag atcctctcca ccttcccctc
     3901 ccgtccgtaa cccctcgaca cgtccggaaa taaaaaaagc ggcggttccg accgccgcgg
     3961 agatatccgt tttcttcggg gaaagatcag cggacgatct ggaattccac ccgccggttg
     4021 cgggaacggc catcgtcggt gttgtt
//
LOCUS       DAOTFD010000226         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16406_length_4038_cov_3.258097, whole genome shotgun sequence.
ACCESSION   DAOTFD010000226 DAOTFD010000000
VERSION     DAOTFD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16406_length_4038_cov_3.258097"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5079_10195"
     CDS             <1..615
                     /locus_tag="P5079_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRY30389.1"
                     /translation="SCQSMRGFGARVMITEIDPICALQAAMEGYEVTTIDEALKEADI
                     FVTATGNNGVITLEHMKKMKDQAIVCNIGHFDSEIEVDKLKSLPGIKHLNIKPQVDKY
                     TFPDGHSIFLLAEGRLVNLGCAMGHPSFVMSCSFTNQTLAQMDLWKNDYKVGVYRLSK
                     KLDEEVARLHLDKLGAKLTRLSKEQADYIGVPVDGPYKPEHYRY"
     gene            794..1753
                     /locus_tag="P5079_10200"
     CDS             794..1753
                     /locus_tag="P5079_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30390.1"
                     /translation="MKKYTAVAAVAALLASLALWGCKNGNMFGGFHKKGSGDAASLMS
                     DARSALANREFNNAKAYYEAILAQQPTNSVALYGAAVATMGTAGLDLGTLLSNVVASK
                     GAPASGSLAQLIDSAAIGAPGAATVDPRSVLNNLNLTALRLHINTIICYLSRIRAGRG
                     DGVIPRDDVSTCLSLTISRTLRAVLRTTDPTVGVADIRKSANGKDLEVVITASGATIN
                     DYCGGTSGKGKTMEATLDDLAGALEALGAAFNKVNPGVDATLTEIKADINKAFTDFKT
                     DVKAQGVNAACAGLLDSAKYDVLNLPVPTTDDCAQLGQACCSQ"
     gene            1784..3130
                     /locus_tag="P5079_10205"
     CDS             1784..3130
                     /locus_tag="P5079_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30391.1"
                     /translation="MKRTLIALLAILMPYAAWSAERPTKIRGVRALGMGDAFTAVADD
                     QNVFFYNPAGITQRTGGMVTLLEIPLTVGTDLLDAYEFIDDKGDKLKDFDQLTPTEQA
                     DLINEIDRTITKLNPHVGVGAPNINYLSGPIADRYYWGFGGFGQVEGSFRVNTGIVPS
                     LDYDINADVVIPVTFAAKWTEVKRIPGKIGAGVNLKYIQRNQIKDERVSVLQLEDFDE
                     PQMQKGTGLGADIGLLYQPNNRWNFALASLDFLGTSLSFDEATAEKGFTAKPSRVSGI
                     KSRWNLGTSWTPTKIVYWPGKSLSTKNRLLLAADVKDIFNAESKVLFGDSFLPDTAWT
                     HVHLGAEYRWWFLRFRGGTNQGYPTYGLGLDLPLLKIDYAYYSDELGLYAGSLEQANH
                     MITVALRFGGSKTEARERVRGEKSGATAAPAAAPVAVPAPTTPTQTPVPVEAAPKQ"
     gene            3443..>4038
                     /locus_tag="P5079_10210"
     CDS             3443..>4038
                     /locus_tag="P5079_10210"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRY30392.1"
                     /translation="MAAPPTNATPKIYVVRVEFQDKLMGKTLIQSQPFFSNMRSFFIE
                     NSYGAFQPTFTLSPTLYKLPKTLNSYGSDTLGNAAYQLLFTDTKALVEGAVDFTQYDE
                     VMIYHAGNGQETSGLSQDIWSVYFPTAQTLDGKSFNGFTVVPETEAGGFDPLGVITHE
                     YGHQLGLPDLYDTAASGGRSTCGAWSVMDYPYTGITTA"
BASE COUNT          897 a         1292 c         1133 g          716 t
ORIGIN      
        1 agctgccaga gcatgcgggg attcggcgcc cgggtgatga tcaccgagat cgatccgatc
       61 tgcgccctcc aggccgccat ggagggctac gaggtcacca ccatcgacga agccctcaaa
      121 gaagcggaca tcttcgtcac cgccacgggc aacaacgggg tgatcaccct ggaacacatg
      181 aagaagatga aagaccaggc catcgtctgc aacatcgggc atttcgattc ggagatcgag
      241 gtggacaaat tgaagagcct gccggggatc aaacatttga acatcaaacc ccaggtggac
      301 aaatacacct ttcccgacgg tcattccatc ttcttgttgg ccgaaggacg tttggtgaac
      361 ctgggctgcg ccatggggca tccctccttc gtgatgtcct gttccttcac caaccagacg
      421 ctggcccaga tggacctttg gaagaacgac tataaggtgg gcgtttatcg gctgtcaaaa
      481 aagctggacg aggaagtggc gcggctgcac ctggacaaac tgggcgcaaa actcacccgg
      541 ctctccaagg aacaggccga ttatatcggc gtaccggtgg acggtccgta caagccggag
      601 cattaccggt attaaaacgc atcggcgcgg aatcgcccgg cgtttccgcc gttctaaagt
      661 cacattgaca cggccccaag aaccctgtta gaatctcacc ggagcgagaa gcttcgcccg
      721 tcatcatcca acgaaatcag cgattctccc gaaatccatt ccggagtcaa cgtctgagga
      781 ggtcattgtt cccatgaaaa aatacaccgc agtcgcggcc gtcgcggccc tcttggcgag
      841 tctggccttg tggggatgca aaaacggcaa catgttcggc ggatttcaca agaaaggcag
      901 cggtgatgcc gcgtcgttga tgtccgacgc gcgttccgcg ctggccaacc gcgaattcaa
      961 caacgccaag gcgtattacg aagcgatctt ggcgcagcaa ccgaccaact ccgtggccct
     1021 ctacggcgcc gccgtggcca ccatggggac ggccggactc gacctgggaa ccctcctctc
     1081 caacgtggtc gcttccaaag gggcccccgc ctccggttcg ctggcgcagc tcattgactc
     1141 agccgccatc ggagcgcccg gagccgcgac ggtggatccc cgcagcgtct tgaacaatct
     1201 gaacctgacc gctctccgtc tccatatcaa caccatcatc tgttatcttt cccggatccg
     1261 ggcggggcgg ggcgacggag tgattccgcg ggacgacgtg agcacctgct tgtccctcac
     1321 catctcgcgc acgttgcggg ccgtcctccg gaccaccgac cccacggtcg gcgtggcgga
     1381 catccgcaag agcgccaacg gcaaagacct ggaagtcgtc atcaccgcct ccggcgccac
     1441 catcaacgat tactgcggcg gcaccagcgg caagggcaag accatggaag ccaccctcga
     1501 cgacctcgcc ggcgcgctcg aagcgttggg cgcggccttc aacaaggtca accccggggt
     1561 ggacgccacc ttgacggaaa tcaaggccga catcaacaaa gccttcaccg acttcaaaac
     1621 cgacgtgaaa gcgcagggcg tcaacgcggc ctgtgccggc ctgctggaca gcgccaaata
     1681 cgacgttctg aatctgccgg ttccgaccac ggacgattgc gcgcaactcg gccaggcttg
     1741 ctgctctcaa taacgactta atcccacaat gaggacacta accatgaaac gcacgttaat
     1801 agcgcttctc gcaatcctta tgccgtacgc ggcctggtcc gcggaacggc cgacaaaaat
     1861 tcggggcgtc cgcgcgctcg gcatgggcga cgcctttacc gccgtggcgg atgaccagaa
     1921 cgtcttcttc tacaacccgg cgggcattac ccaacggacc ggtgggatgg tcacccttct
     1981 ggaaatccct ctcacggtcg ggacggacct cctcgacgct tacgagttca tcgacgacaa
     2041 aggggacaaa ctgaaggact tcgaccagtt gacgccgacg gaacaggccg acctcatcaa
     2101 cgagatcgac cggaccatca cgaaactcaa tccccacgtg ggcgtcggag cgccgaacat
     2161 caactacctg tccggcccca tcgcggaccg ctactactgg gggttcggcg ggttcggtca
     2221 ggtggagggg agtttccgtg tcaacacggg catcgtcccc agcctcgact acgacatcaa
     2281 cgcggacgtc gtcatcccgg tgacctttgc cgccaaatgg acggaggtga agcgcatccc
     2341 cggcaagatc ggcgcggggg tcaacctcaa atacatccaa cggaaccaga tcaaggacga
     2401 gcgcgtctcg gtgcttcaat tggaagactt cgacgagccg cagatgcaaa aagggaccgg
     2461 cctgggcgcc gatatcggcc tcctctacca gccgaacaac cggtggaact tcgccctggc
     2521 gtcgctggac ttcctcggca ccagcctttc ctttgacgag gcgacggccg aaaaggggtt
     2581 caccgccaaa ccgtcccgcg tcagcggcat caaatcccgc tggaacctcg gcacctcctg
     2641 gaccccgacg aaaatcgtgt attggccagg gaaatccctc tccaccaaaa atcggctcct
     2701 tctggcggcc gacgtcaagg acatcttcaa cgcggagagc aaggtgcttt tcggcgacag
     2761 tttcctgccg gacaccgcct ggacgcacgt ccacctggga gcggaatatc gctggtggtt
     2821 cctgcggttc cgcggaggca ccaaccaagg gtatcccacg tatggcctcg gactggatct
     2881 gcccctgttg aagatcgact acgcctatta cagcgacgaa ctcggcttgt atgcgggctc
     2941 gttggaacag gccaaccaca tgatcaccgt ggccctgcgt ttcggtggaa gcaagacaga
     3001 agcccgggaa cgtgtccgcg gcgaaaaatc gggcgcgacg gccgcgccgg cggcggcgcc
     3061 ggtggctgtt ccggccccga cgacacccac gcagacgcct gttcccgtgg aagcggctcc
     3121 gaaacaataa gcggtttata agaaaaacgg caagtttaga aaaaacatcc ccgggaaacc
     3181 ggggatgttt ttttgggtgg gtttctgtca caaaaaggta aaataacccc gttgcatttt
     3241 ctgtaggaag gacacggtgg gaaaaactca aaagcttcat attctctgtg cgtttctttt
     3301 gacggcgcaa ctcccgctcg cggcggcgcc ggcggccccg ggtgtaaaat ctccggaggg
     3361 ccgccccacc tgttatatca acgcggaagc cgcccgcgcg cgcgtccgcg cgctcaaaac
     3421 tgtccgtacc cacggagccc cgatggcggc ccctcccacc aacgcgaccc ccaagatcta
     3481 cgtcgtccgt gttgagttcc aagacaaact catgggaaaa accctcatcc agtcgcagcc
     3541 ctttttttct aacatgcggt cgttctttat cgaaaactcc tacggcgcgt ttcagcccac
     3601 tttcaccctc agccccacgc tgtataaact tcccaaaacc ctgaactcct acgggtcaga
     3661 caccctcggg aacgccgcct accaattgtt attcacggac acaaaggcgc tggtggaggg
     3721 ggcggtcgat ttcactcaat acgatgaagt gatgatctat cacgcgggca acgggcagga
     3781 aacgtccgga ttgagccaag acatctggtc ggtgtatttc ccgacggccc aaaccctgga
     3841 cggaaaaagt ttcaacgggt tcaccgtggt gccggaaacg gaggcggggg ggttcgatcc
     3901 tctcggtgtc atcacccacg aatatggcca tcaactgggc ctgcccgacc tctacgacac
     3961 cgccgccagc ggcggccggt ccacctgcgg cgcctggtcg gtgatggatt atccctacac
     4021 cggcataacc acggcgga
//
LOCUS       DAOTFD010000227         4018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16524_length_4018_cov_4.180924, whole genome shotgun sequence.
ACCESSION   DAOTFD010000227 DAOTFD010000000
VERSION     DAOTFD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16524_length_4018_cov_4.180924"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5079_10215"
     CDS             <1..810
                     /locus_tag="P5079_10215"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRY30393.1"
                     /translation="FEPSTRTRASFELAAKRLSADTLNIAVSASSVQKGESLLDTAKT
                     LEAMKVDFLVLRHASSGAAELVAKGVKARVINAGDGTHEHPTQGLLDMYTILEKKGRL
                     AGLRVAIVGDILHSRVARSNLWGLLKMGAQVTVCGPSTLIPPGVDKAFGKTVRVTHRL
                     DEALADADVVNVLRLQRERQEGNFFPSLREYTELYGLTPDRLKKAKPGCLVLHPGPMN
                     RGIEIASEVADGPQSVIWEQVTNGIAVRMAVLHLFSGPEGFSMEKAIETAR"
     gene            818..2134
                     /locus_tag="P5079_10220"
     CDS             818..2134
                     /locus_tag="P5079_10220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRY30394.1"
                     /translation="MKEKSSDNKVPRADVLLIRGGRVVDPAHKRDGVMDVLMENGKIA
                     RVGKNLAVSPAVETVDARGLVVIPGLVDMHVHLREPGNEDAETIESGTRSAAAGGITG
                     VAAMPNTNPPPDNVSGIRFLLRKAAEEGAVRVWPVGTITHNRAGKALSEIGAMSQAGC
                     VALSDDGSAVADSQVLRRAMEYARLFHLTVIEHAEDKALFSDGVMNDGPLATRLGYRG
                     IPRQAEYVVVARDIALAELTGARLHLAHLSTRESVDLVRQAKRRGLAVTAEVTPHHFT
                     LTEEAVVRYGTNAKINPPLRTKDDREALWEGLADGTIDAIASDHAPHIRSAKEQEFAA
                     APFGIVGLETLLPLTVTQLIDKKILSWPEAVRKMCWNPARILNVPAGHLGAGAPADVT
                     LVDPRKSRKVMSFSSRSDNSPFLGWALRGFARMVFVGGRRVFGTVA"
     gene            2135..3271
                     /locus_tag="P5079_10225"
     CDS             2135..3271
                     /locus_tag="P5079_10225"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRY30395.1"
                     /translation="MKREESTGSFGTEVYKKIFRPVLFRFPPEMVHGLVLEGLNVSSA
                     VGLGGLFRKYLSVRDEALQVSLATDSGRLDFPNPVGLAAGFDKDASAAGALEGLGFGF
                     LEVGTLTPRPQKGNPRPRLRRFPADSALWNRMGFNNIGAERVASRFPRRRRLGIPVGF
                     NIGKAKNTPLEAAAGDYLKSVELLFPWADFFVLNVSSPNTAGLRELQAAAVLERLLKA
                     VRRRMEELALKSNRAGRPALFVKVSPDESSPDTLAETVLRGGAAGIVATNTTVRRDGL
                     SGPVPPDGGVSGRPLRERSTDMLRRLYRASRGRLFLIGVGGVFTAEDAYEKVLAGASL
                     VEVYTGLVYEGPALARDINRGLLTLLKRDGFSSVREAVGKGVKI"
     gene            3268..3948
                     /gene="pyrF"
                     /locus_tag="P5079_10230"
     CDS             3268..3948
                     /gene="pyrF"
                     /locus_tag="P5079_10230"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRY30396.1"
                     /translation="MKRKGKGLIVALDVDSPASARALVRRLGGSVDFYKIPPALSLRD
                     PTLVPWLRRAGKKMFLDHKWYDIPSQMRRSVQAAGRAGVTSCTVHVSAGLAALRAAAS
                     LRPRPLLWGVSVLTSFTNRDLRDIGVGSSVPAQVCRLARLAEKAGLEGLICSPQELPL
                     LKKTSLTLVTPGIRLGRASARDDQARSMTPREAWSNGARWIVVGRPILESGDPLAAVR
                     EILRERPC"
BASE COUNT          669 a         1180 c         1405 g          764 t
ORIGIN      
        1 ttcgagccgt ccacccgtac gcgggcgtct ttcgagttgg ccgccaaacg gctctccgcc
       61 gatacgctca acatcgccgt gtccgccagc tccgtgcaga aaggggaatc cctcctcgat
      121 accgccaaga cgttggaggc gatgaaggtg gattttctgg tgttgcgcca tgcctcctcg
      181 ggggcggcgg agctggtggc caagggcgtg aaggcccgcg tgataaacgc cggggacggc
      241 acccacgaac accccacgca gggacttctc gacatgtaca ccatcttgga aaaaaaaggc
      301 cggctggccg gcctccgcgt ggccatcgtg ggagacatcc tccattcccg ggtggcccgc
      361 tccaatctgt gggggctttt gaaaatgggg gcgcaggtca ccgtttgcgg tccttccacg
      421 ctcatcccgc cgggggtgga taaggctttc gggaaaacgg tgcgggtcac gcatcgtttg
      481 gacgaggcgc tggcggacgc ggacgtggtc aacgtgctcc gactgcagcg cgaacggcag
      541 gaagggaatt tttttccgag cctgcgcgaa tacaccgaac tttacggtct cacgccggac
      601 cgtctgaaga aggccaagcc gggatgtctg gtgttgcatc cggggcccat gaaccggggg
      661 atcgagatcg cctccgaggt ggcggacggc ccgcagtccg ttatctggga acaggtgacc
      721 aacggcatcg cggtgcggat ggcggtgttg catctcttct ccgggcctga gggattttcc
      781 atggagaaag ccattgagac ggcgaggtga ggggacgatg aaagagaaaa gctcggataa
      841 caaagtgccg agagcggacg ttttgttgat ccgcggcggt cgcgtcgtcg atcccgccca
      901 taaaagggac ggcgtcatgg acgtgctgat ggaaaacggg aagattgccc gggtggggaa
      961 gaacctggcg gtttcgccgg ccgttgagac ggtggacgcg cgggggctgg tcgtcatccc
     1021 cggtctcgtg gatatgcacg tccatctccg agagcccggc aacgaggacg ccgaaacgat
     1081 tgaatccggc acgcgttcgg ccgccgcggg cggcatcacc ggcgtggccg ccatgcccaa
     1141 caccaacccg ccgccggaca atgtgtccgg cattcggttc cttttgagga aggcggccga
     1201 ggagggcgcg gtgcgcgtgt ggcccgtcgg gaccatcacc cataaccgcg cgggcaaggc
     1261 gttgtccgag atcggcgcca tgtcccaggc cggctgcgtg gccctttcgg acgacggctc
     1321 cgccgtcgcc gacagccagg tgttgcgtcg ggccatggag tatgcccggc tgttccatct
     1381 gacggtcatc gagcatgccg aagacaaggc cctgttctcg gacggcgtga tgaatgacgg
     1441 cccgctggcc acccgcctgg gataccgcgg catcccccgt caggcggaat acgtcgttgt
     1501 ggcgcgggac atcgccttgg ccgagttgac cggcgcccgg ctgcacctgg cccacctctc
     1561 cacgcgggag tcggtggatc tggtgcgtca ggcgaaacgg cgagggcttg ccgtgacggc
     1621 ggaggtgacg ccgcaccatt tcaccctcac cgaagaggcg gtggtccgtt acggcaccaa
     1681 cgccaagatc aatccgcccc ttcggacgaa agacgaccgg gaagcgctgt gggaaggtct
     1741 ggccgacggc accatcgacg cgattgccag cgaccatgcc ccgcacattc gctcggccaa
     1801 ggaacaggag ttcgcggcgg cgccgttcgg catcgtcggg ctcgagaccc ttctgccgtt
     1861 gacggtgacg cagttgatcg ataagaagat cctttcctgg ccggaggcgg tgaggaagat
     1921 gtgctggaac ccggcgcgga tattgaacgt gcccgccgga catctggggg ccggcgctcc
     1981 cgccgatgtg accttggtgg acccgcgaaa gagccggaag gtgatgtcct tctcgtcccg
     2041 cagcgacaat tcgccgtttc tgggatgggc cctgcgtggt tttgcccgca tggtttttgt
     2101 gggggggcgg cgggtgttcg ggaccgtcgc gtagatgaaa cgggaggaat ccaccggatc
     2161 tttcggaacg gaagtctata aaaagatttt tcgcccggta ttgttccggt tcccgccgga
     2221 gatggttcac gggctggtcc tcgaggggct caacgtttct tccgccgtcg ggctgggcgg
     2281 gctctttcga aaatatttgt cggtgaggga tgaggcgttg caagtttccc tggcgacgga
     2341 ttccggccgg ttggattttc cgaaccccgt cggtctggcc gccgggttcg acaaggacgc
     2401 gtcggcggcc ggcgcgctgg aagggttggg attcggattt ttggaagtcg gaacgctcac
     2461 gccgcgtccg cagaagggga acccgcgtcc gaggctccgg cggttcccgg ccgattcggc
     2521 cctctggaac aggatggggt tcaataacat cggcgcggaa cgggtcgctt cgcgttttcc
     2581 acggcgccgt cgactcggga ttcccgtggg tttcaatatc gggaaggcga agaacacgcc
     2641 gttggaggcc gccgccggcg attacctgaa gtccgtggaa cttcttttcc catgggcgga
     2701 ttttttcgtt ctcaacgtca gttcgccgaa caccgcgggg ttgcgcgaac ttcaggccgc
     2761 ggcggttttg gagcgtttac tgaaggccgt tcggcggcgg atggaagagc tcgctcttaa
     2821 atcgaatcgc gccggccggc cggcgctctt tgtcaaagtc tccccggatg agtcgtcacc
     2881 ggacaccttg gcggaaacgg ttcttcgcgg cggggcggcg gggatcgtcg cgaccaacac
     2941 caccgtgcgg cgcgacggcc tctccggtcc ggtccctccg gacggcggcg tcagcggacg
     3001 tccgttgcgc gagcggtcga cggatatgct ccgccggctc tatcgcgcct cgagaggaag
     3061 gttgtttttg atcggcgtgg gcggcgtttt caccgcggaa gacgcgtacg aaaaggtcct
     3121 cgccggcgcc tccctggtgg aggtctacac gggattggtc tatgaggggc cggccctggc
     3181 ccgggacatc aatcggggtc tgttgacgtt gttgaaacgc gacggttttt cctctgtccg
     3241 ggaagccgtg ggaaagggcg tgaagatatg aaacggaagg gaaaagggtt gatcgtggcg
     3301 ttggacgtgg attcgccggc gtcggcgcgg gcgctggtcc gccggctggg cggctcggtg
     3361 gatttctaca agatcccgcc cgcgctgtcc cttcgcgatc ccacgctggt tccctggctc
     3421 cggcgggccg gcaaaaaaat gttcctggac cacaaatggt atgatattcc gtctcagatg
     3481 cgccgttccg tgcaggccgc cggccgcgcc ggggttactt cctgcacggt ccacgtgagc
     3541 gccggtctcg cggccttgag ggcggccgcg tctctccggc cccggcctct cctgtggggg
     3601 gtcagcgttt tgacgagttt tacgaacagg gatttgcggg acatcggggt gggttcttct
     3661 gtgcccgcgc aggtgtgccg cttggcccga ctggccgaga aggccggttt ggaggggttg
     3721 atctgttctc cgcaagaact accgttgctg aaaaaaacgt cgttgacgct ggtaacgccg
     3781 gggatccgtc tcggccgggc gtccgcgcgc gacgaccagg cccggagcat gacgccgcgg
     3841 gaagcatggt cgaacggcgc gcggtggatt gtcgtggggc ggcccatcct ggaatccggg
     3901 gatcctctcg ccgccgttcg tgaaattttg agggagaggc cgtgttgacg gaaaaagagg
     3961 tccggcagat attggaggcc gcgcgcgccc tcatgcaggg gcatttccaa ctttccag
//
LOCUS       DAOTFD010000228         3995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16663_length_3995_cov_3.435787, whole genome shotgun sequence.
ACCESSION   DAOTFD010000228 DAOTFD010000000
VERSION     DAOTFD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16663_length_3995_cov_3.435787"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5079_10235"
     CDS             complement(<1..231)
                     /locus_tag="P5079_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRY30397.1"
                     /translation="MKTNKPTLDRIVACKKAELAATKAHCPFPELEKRLNQSPPLRDF
                     AESIRSADGVALVAELKKASPSACRSACLSNSE"
     gene            complement(228..1259)
                     /gene="trpD"
                     /locus_tag="P5079_10240"
     CDS             complement(228..1259)
                     /gene="trpD"
                     /locus_tag="P5079_10240"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRY30398.1"
                     /translation="MIQETVVRLVEKNSLSSPEAREAMEEILSGTATPSQIAGFLTAL
                     RMKGETPEEIAGCAEAMRGAATRIPLETPVIVDTCGTGGDKKGAFNVSTAAAFVVAGA
                     GFTVAKHGNRSISSQCGSADVLEELGVKIDPPVAVVERCLREIGIGFMFAPAFHPAMR
                     HAMPTRRELGVRTVFNILGPLCNPAGANVQVLGVYSPDLLDVLAQVLVRLGMKHSMVV
                     HGGGYDEITLSGETLAAEIVDGRVQRIKLSAKDFGVPAHPDTVLRGGDKKSNAEILRK
                     ILKGIRGPQRDVVVANAGTAILLASRAAGKKTPRNRDEACREAERVLDSGAALKKLDR
                     LAELSHAAS"
     gene            complement(1264..1842)
                     /locus_tag="P5079_10245"
     CDS             complement(1264..1842)
                     /locus_tag="P5079_10245"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRY30399.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELRQRVKVVRNDAISADDVGRLKP
                     RRILISPGPCTPKEAGISVDVIRKYAGKIPILGVCLGHQSLGYAFGGKVVRAQRLMHG
                     KTSKIKHDGKGVFRNVPRPFTATRYHSLVVERKSLPRDLEISATAGDGEIMGLRHKHL
                     PCVEGVQFHPESILTEHGKTILANFVKMRIRK"
     gene            complement(1839..3320)
                     /gene="trpE"
                     /locus_tag="P5079_10250"
     CDS             complement(1839..3320)
                     /gene="trpE"
                     /locus_tag="P5079_10250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRY30400.1"
                     /translation="MIHPSFDEFKRLAKKHDGVPVFLECLADELTPMALFHSLYEKSP
                     RCFLLESVEGGEHLGRYSFAAFEPFQVFKSRARECELETGGRSRKWESENPFNELRGL
                     LRSRRAAEVKGLPRFFGGAVGYASYDVVRHFERLPDNHPDDLRTPDMFFFFTRDMFVF
                     DHLAHSVKIVRCLFPKKGDDLRALYRRAERALAKTARDIRLAPFRAKPVSPPPPAAAK
                     EDPRERAAFLAAVRKAKDYIAAGDIIQTVLSRRVSFPTKAHPVEIYRALRCVNPSPYM
                     YFLRDKDTHLIGTSPEMLVRLEDRTAETRPIAGTRPRGKNAPDDDRLAAELVRDEKER
                     AEHLMLVDLGRNDLGRVCRPGTVRVPDFMTVERYSHVMHLVSSVVGQLDPRRDAFDLF
                     RACFPAGTLSGAPKIRAMEIIDELESARRGPYGGAVGYFSYSGNMDVAITIRTILLAR
                     KAAHVQAGAGIVADSVPEKEYRETQNKAAAMLAAIRLAETRRT"
     gene            complement(3317..3823)
                     /gene="lepB"
                     /locus_tag="P5079_10255"
     CDS             complement(3317..3823)
                     /gene="lepB"
                     /locus_tag="P5079_10255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY30401.1"
                     /translation="MKRWLFLFALGALGAWVVRGGMVEGIVVATASMEPTLPVGTHLF
                     VNKAAYRFRAPRRGEIVVFPSPVGQKDMVKRIIAVEGDTVELRKKKVYLNDKPLDEPY
                     VRHTRPDEILQGDNVAPRVVPRKRVFVLGDNRDQSGDSRDWVDPKTGEHVYFIHVNKL
                     KGKLMGVQ"
     gene            complement(3823..>3995)
                     /locus_tag="P5079_10260"
     CDS             complement(3823..>3995)
                     /locus_tag="P5079_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRY30402.1"
                     /translation="LDCDKDCLLVKVNQIGGAACHTGRRSCFFHHVEQDGKLEIRGEL
                     LFDPAKVYKNKK"
BASE COUNT          677 a         1221 c         1290 g          807 t
ORIGIN      
        1 ttcagaattg gacaggcagg cggacctgca cgcggacggg ctggcctttt tcagttccgc
       61 gacgagagcc accccgtcgg cggagcggat cgattcggcg aaatcccgca ggggcggcga
      121 ctggttcagc cgtttttcaa gttccgggaa cggacaatgg gccttggtcg ccgccaactc
      181 cgcttttttg caggcgacga tgcggtccag cgtcggcttg ttcgtcttca agaggcggcg
      241 tggctcagtt cggcgagccg gtccagtttt ttcaatgccg ctcccgaatc caggactcgt
      301 tccgcttccc ggcaggcctc gtcccggttc cgcggggtct ttttgcccgc ggcgcgcgag
      361 gccaaaagga tggcggtccc ggcgttggcg accacgacat cgcgttgggg gccccggatc
      421 cctttcagta ttttccgaag gatctccgcg ttcgattttt tgtcgccgcc gcgcaagacc
      481 gtgtccggat gcgccgggac gccgaaatcc ttggccgaca gttttatccg ttggacccgg
      541 ccgtccacga tctccgccgc cagggtttcg ccggaaaggg tgatctcgtc gtagccgccg
      601 ccgtggacca ccatggaatg tttcatgccc agccggacca aaacctgggc caaaacgtcg
      661 aggaggtctg gggaatagac cccgagcacc tggacgttgg cccccgccgg attgcagagc
      721 ggccccaaga tgttgaaaac ggtgcgcact cccaattccc ggcgggtggg catggcgtgg
      781 cgcatggccg gatggaacgc cggggcgaac atgaagccga tgccgatctc gcgcaggcag
      841 cgttccacca cggccacggg cgggtctatt ttcacgccca gctcttccag gacgtcggcg
      901 ctgccgcatt gagacgagat ggaccggttc ccgtgcttgg ccacggtgaa cccggcgccc
      961 gccaccacga acgccgccgc ggtcgacacg ttgaacgcgc ccttcttgtc tcctccggtc
     1021 ccgcaggtgt cgacgattac gggcgtttcc agggggatgc gcgtggccgc gccgcgcatg
     1081 gcttcggcgc agccggcgat ttcctcgggg gtctcccctt tcatccgcag agccgtcaag
     1141 aaaccggcga tctgcgaggg ggtggcggtt ccggacaaaa tctcttccat cgcttcccgg
     1201 gcttccgggg aggagaggga gtttttttcg acgagccgga cgacggtttc ctggatcatg
     1261 ggattatttc cggatcctca ttttaacgaa attcgcgagg attgtctttc cgtgttcggt
     1321 gaggatggat tccgggtgga actgaacgcc ttcgacgcag ggcagatgtt tatggcgcag
     1381 ccccatgatc tcgccgtctc ccgccgtggc cgatatctcc aggtcccgcg gaagggactt
     1441 cctctccacc accagggaat ggtagcgggt ggccgtgaac ggacggggga cgttccgaaa
     1501 aacccctttt ccatcgtgtt tgatcttcga ggtcttcccg tgcatcaggc gttgggcgcg
     1561 aacaaccttt ccgccgaagg catagccgag ggactgatgt ccgaggcaga cgccgaggat
     1621 cggtatttta ccggcgtatt tccggatgac gtccaccgag atcccggctt ccttgggggt
     1681 gcaggggccg ggcgaaatga ggatccggcg cggtttcaac cggccgacgt cgtccgcgga
     1741 gatggcgtcg ttccgaacga ctttcacccg ttggcgcagt tcgccgaggt attgcaccag
     1801 gttgtaggtg aaggaatcgt agttgtcgat gaccaggatc atgttcgtct cgtttccgcc
     1861 aaacggatgg ccgcgagcat ggcggcggct ttgttttgcg tctcccgata ttccttttcc
     1921 gggacggagt cggccacgat gcccgccccc gcctgaacgt gggcggcctt ccgtgcgagg
     1981 aggatggttc ggatggtgat ggccacgtcc atgttgccgg aataggaaaa gtaaccgacg
     2041 gcgccgccgt acgggccgcg ccgggcgctc tcgagttcgt cgatgatctc catggcgcgg
     2101 atcttcggcg ctcccgacag cgttccggcc gggaagcagg cccggaacag gtcgaaggcg
     2161 tcgcggcgag ggtccagttg tcccaccacg ctggagacca aatgcatcac gtggctgtag
     2221 cgttcgacgg tcatgaaatc ggggacgcgg acggtgccgg gccggcagac ccgtcccagg
     2281 tcgtttcggc cgaggtccac cagcatcaaa tgttcggccc gttctttctc atcgcggacc
     2341 aattccgcgg cgagccggtc gtcgtccggc gcgtttttcc ctcgggggcg ggtgccggcg
     2401 atgggccggg tctcggcggt gcggtcctcc aggcgcacca acatttcggg actggtgccg
     2461 atgaggtggg tatccttgtc ccgaaggaaa tacatgtagg gcgacgggtt tacgcaacgc
     2521 aacgcccggt agatctcgac ggggtgggct ttcgtgggga aggagacccg gcgcgacagg
     2581 accgtctgga tgatgtcccc ggcggcgata tagtctttcg cttttcggac ggcggccagg
     2641 aaggcggccc gttcccgggg gtcctctttc gccgcggcgg gcggcggggg cgagaccggt
     2701 tttgcccgga acggcgccag acggatgtcc cgggccgttt tggccagggc tcgctcggcc
     2761 cggcggtaaa gcgcgcgcag gtcgtcccct tttttgggga agagacaccg cacaatcttc
     2821 acggaatgcg ccagatggtc gaagacgaac atgtcccggg tgaagaaaaa aaacatatcc
     2881 ggcgtgcgga ggtcgtcggg gtgattgtcc gggaggcgtt cgaagtggcg caccacgtcg
     2941 taactggcat agccgacggc gcctccaaag aagcgcggca ggcctttcac ctccgccgcc
     3001 cgtcggctcc ggaggagtcc gcgcaattcg ttgaaagggt tctccgattc ccacttccgg
     3061 gaccgaccgc cggtttccag ctcacattcg cgcgcccggc tcttaaaaac ttgaaacggt
     3121 tcgaaggcgg cgaaagaata ccgtccgaga tgctcccctc cctccacgct ctccaggagg
     3181 aaacagcgcg ggctcttctc atagagggag tggaacagcg ccatgggggt gagctcgtcc
     3241 gccaggcatt ccaggaacac cggaacgccg tcgtgttttt tcgccaaacg tttgaattcg
     3301 tcgaaactcg gatgaatcat tggactccca tcaatttccc tttcagtttg ttcacgtgga
     3361 tgaaatagac atgttccccg gtcttcgggt ccacccagtc gcggctgtcg ccggattggt
     3421 cgcggttgtc gcccaggacg aacacgcgtt ttcgggggac gacgcgcggc gcgacattgt
     3481 cgccctggag tatttcgtcc gggcgggtgt gtcggacgta cggttcatcc agcggcttgt
     3541 cgttcaaata gacctttttt ttccgcaact ccaccgtgtc gccttccacg gcgatgatcc
     3601 gtttgaccat gtctttctgt cccacggggg acggaaagac cacgatctcc ccccgtctcg
     3661 gcgcacgaaa gcgataggcc gccttgttga cgaaaaggtg cgtgcccacc ggcaacgtgg
     3721 gctccatgga ggcggtggcc accacgatgc cttccaccat gcctccgcgc acgacccatg
     3781 cgccgagcgc ccccagggcg aacaggaaga gccaacgctt cactatttct tgttcttata
     3841 gactttcgcc ggatcgaaca gaagttctcc ccgtatctcg agttttccgt cctgttcgac
     3901 gtggtgaaaa aagcaggacc ggcggccggt gtggcaggcg gctccaccga tctggttcac
     3961 tttcaccagg aggcagtcct tgtcgcagtc caggg
//
LOCUS       DAOTFD010000229         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16887_length_3961_cov_4.632104, whole genome shotgun sequence.
ACCESSION   DAOTFD010000229 DAOTFD010000000
VERSION     DAOTFD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16887_length_3961_cov_4.632104"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="P5079_10265"
     CDS             <1..584
                     /locus_tag="P5079_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRY30403.1"
                     /translation="GVEWRCLKEALLYDLKRGRLARGGSTITQQLAKNLYLSPSKNPL
                     RKVRELVLTYRLEKVLSKRRIFEVYLNVVEWGSGIFGAEAAARAYFGKPASELTLDES
                     ASLAAILPSPRRHDPRSGSRWVELRKQWVYRRMAESQRLPDLSAPSAEPEPPPPPPEE
                     DEPETEEGEVAVESSTGPETAPASRNPAPPSPL"
     gene            647..1225
                     /locus_tag="P5079_10270"
     CDS             647..1225
                     /locus_tag="P5079_10270"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRY30404.1"
                     /translation="MAYAAKDYSKLIGMEGFSETLLKNHFTLYQGYVTNTNKLIETLD
                     RYLKEDKAGTPEFAELKRRFGWEFNGMRLHEYYFDNLGGKGGPDKNGKLAKKLAESFG
                     SYENWEKDFRATGAMRGIGWVVLYQDPVGGQLFNVWINEHDVAHPAGCAPLLVMDVFE
                     HAFMIDYGLKRADYIAAFFKNVNWSAVQDRVK"
     gene            1289..1768
                     /locus_tag="P5079_10275"
     CDS             1289..1768
                     /locus_tag="P5079_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRY30405.1"
                     /translation="MQNGTIARLLFVCTGNACRSVMAEQLCRRHLAKEGVEGVTVSSA
                     GVAARPEMSVPAEVPRALAPHGVDELRHRPRLLSREMVEEADLILVMDAQQQTTIALR
                     FPAAVGKTRVFKSYVGLTGPSGIADPIGRPQKDYDACARDILAAVVKLVDQLKKGPA"
     gene            1787..3034
                     /gene="glyA"
                     /locus_tag="P5079_10280"
     CDS             1787..3034
                     /gene="glyA"
                     /locus_tag="P5079_10280"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY30406.1"
                     /translation="MTVLQKTDAKVYRAILQETRRQQEGLELIASENFVSEAVLEAQG
                     SVLTNKYAEGYPGKRYYGGCEYVDVAETLAIERAKALFGAEHVNVQPHSGTQANVAVY
                     LSVLNIGDTLMGLELAHGGHLTHGHPMNFSGKHYKVVSFGVHPETEQIDYDRAAQLAL
                     ENKPKLICAGASNYSRVFDWPRLRQIADSVGAVLMTDIAHYAGLVAAGLYPSPVPHAD
                     FVTTTTHKTLRGPRGGMILCREKYAKAIDSAVFPGTQGGPLMHIIAAKAVAFGEALKP
                     EFKRYQKQVLANARALAAALQKRGFRIVSGGTDCHLLCADLRSKSITGKDAQEVLGKA
                     GLTVNKNTIPFDPEKPFIASGIRLGTPAVTTRGMKEPEMEKIAGWIMEAVEHRADEKR
                     LRHVRKQVEDLCRRFPLYPKRRK"
     gene            3081..3716
                     /gene="upp"
                     /locus_tag="P5079_10285"
     CDS             3081..3716
                     /gene="upp"
                     /locus_tag="P5079_10285"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRY30407.1"
                     /translation="MPKKRLIVLDHPLIQHKLTMLRDRTTNSRDFRDLLDEISTLMAY
                     EVTRHLPTQARTVTTPLMRARGKMIPGKMLGVVVILRAGLGMVDGILKLVPGAKVGHI
                     GLYRDPQTMRPIEYFCKFPPDVPKRFMILVDPMLATGYSASAALDILKRAGARKVSLM
                     CLLSCPEGLRVVQRRHPDVPIFTCAVDKKLNSHGYILPGLGDAGDRLFGTR"
     gene            3764..>3961
                     /locus_tag="P5079_10290"
     CDS             3764..>3961
                     /locus_tag="P5079_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30408.1"
                     /translation="MLQIYLIALFCALAGAAFFTARIAGGLIASPTLRTPSTNWIRPL
                     RPRRNTPRQCGVSRIAWAGQTK"
BASE COUNT          764 a         1284 c         1266 g          647 t
ORIGIN      
        1 gcggcgtgga gtggcggtgt ctcaaggagg cgctgctgta cgatctcaaa agagggcgcc
       61 tggcccgcgg cggaagcacc atcacgcagc aactggccaa gaacctttat ctgtctccgt
      121 cgaagaaccc cctccggaaa gtgcgcgaac tggtcctcac ctatcgtttg gaaaaggtcc
      181 tctccaaacg gcggatcttc gaggtctacc tgaacgtggt ggagtggggg agcggtattt
      241 tcggggcgga ggcggccgcg cgcgcctatt tcggcaagcc ggcgtcggag ctgacgctgg
      301 acgagtccgc gtcgctggcg gcgattttgc ccagcccccg ccgccacgac ccccggagcg
      361 gctcccgctg ggtggaactg agaaaacaat gggtctaccg gcggatggcg gaaagccagc
      421 gtctgccgga cctgtccgcg ccgtcggcgg agccggagcc gccgccccct ccgccggagg
      481 aggacgagcc ggagacggag gagggggagg tggcggtgga gagctcgacc ggacccgaaa
      541 ccgccccggc gtctcggaac ccggcgccgc cgtcgccgtt ataagcattg attggatgtt
      601 gtaaaaaaaa atcctaaaat ccacggcaca caaacggagg tgccccatgg catacgcggc
      661 gaaggactat tcgaaattga tcgggatgga gggtttcagc gagaccctgc tgaagaacca
      721 tttcaccctc tatcagggat acgtgaccaa caccaacaag ctcatcgaga cgttggaccg
      781 gtatctgaag gaggacaagg ccggcacgcc ggagttcgct gaattgaaac ggcggttcgg
      841 atgggagttc aacggcatgc gcctgcacga atattatttc gacaacctgg gcggcaaggg
      901 cggacccgac aagaacggca agttggccaa gaaactggcg gagtcgttcg gctcctacga
      961 gaactgggag aaggatttcc gcgccaccgg cgccatgcgg ggcatcgggt gggtggtcct
     1021 ctatcaggat cccgtgggcg gccagctgtt caacgtgtgg atcaacgagc acgacgtcgc
     1081 ccatccggcg gggtgcgcgc ctcttctggt gatggacgtg ttcgaacacg ctttcatgat
     1141 cgattacggc ctgaagaggg cggactacat cgcggcgttt ttcaagaacg tcaattggtc
     1201 cgcggttcag gatagggtaa aatagttttt cgataccgag accgtttcgg ccccgcgcgg
     1261 ggccgttaga aggaggaggg ccctctcctt gcagaacggc acgatcgcgc gcctgttgtt
     1321 cgtgtgcacg ggcaacgcct gccggagcgt gatggcggaa cagctgtgcc gccgtcatct
     1381 ggcgaaggaa ggcgtggaag gcgtcacggt gtcgtcggcc ggcgtggcgg cccggccgga
     1441 gatgtcggtc ccggcggaag ttcctcgcgc cctggcgccg cacggcgtcg acgagcttcg
     1501 tcaccgaccc cgcctccttt cccgggaaat ggtggaggag gccgacctga tcctcgtcat
     1561 ggacgcccag caacagacga cgatcgcttt gcggttcccg gccgcggtgg ggaagacccg
     1621 ggtgttcaaa agctacgtgg ggttgaccgg accctccggc atcgccgacc ccatcggccg
     1681 gccccagaaa gactacgacg cctgcgcccg ggacatcctg gccgcggtcg tgaaactcgt
     1741 cgaccaattg aaaaaaggac ccgcgtaaaa caagagagga gaacgcatga cggttttgca
     1801 gaagacggac gccaaggtgt accgcgccat ccttcaggag acgcgccggc agcaggaggg
     1861 gctggaactc atcgcctccg agaacttcgt ttccgaggcg gtgctggaag cgcagggttc
     1921 cgtgctcacc aacaagtacg ccgagggata tcccgggaaa cgctactacg gcgggtgtga
     1981 atacgtggac gtggccgaga ccctggccat cgagcgggcg aaggccctct tcggcgccga
     2041 acacgtgaac gtgcagcccc attccggcac ccaggccaac gtcgccgtgt acctctccgt
     2101 gctgaacatc ggcgacaccc tcatggggct ggaactggcc cacgggggcc acctcaccca
     2161 cgggcatccc atgaattttt cagggaaaca ttacaaggtg gtctcgttcg gcgtgcaccc
     2221 ggagacggag cagatcgatt acgaccgcgc cgcccagttg gcgctggaga acaagcccaa
     2281 gctgatctgt gcgggcgcct ccaactattc gcgcgtgttc gactggccgc gcctgcggca
     2341 gatcgcggat tccgtcggcg ccgtgctgat gaccgacatc gcccactacg ccggcctggt
     2401 ggcggcgggg ctctacccct cgccggtgcc gcacgcggat ttcgtcacca ccaccacgca
     2461 caagaccctc cgcggccccc gcggcggcat gatcctgtgc cgggagaaat acgccaaggc
     2521 catcgacagc gccgtcttcc ccggcacgca gggcggtccg ctcatgcaca tcatcgccgc
     2581 caaggccgtc gcgttcggcg aggccctgaa accggagttc aaacgctacc agaaacaggt
     2641 gctggccaac gcccgcgccc tcgcggccgc cctgcagaaa cggggcttcc gcatcgtttc
     2701 gggcggcacc gactgtcacc tcctctgcgc cgacctgcgt tccaagtcca tcaccgggaa
     2761 agacgcccag gaggtcctcg gcaaggccgg cctcaccgtc aacaagaaca ccatcccgtt
     2821 cgacccggag aagccgttca tcgcctccgg catccgcctg ggtacgcccg cggtcaccac
     2881 gcgcgggatg aaggagccgg agatggaaaa gatcgccggg tggatcatgg aggcggtcga
     2941 acatcgcgcg gacgaaaaac gcctccggca tgttcgaaag caagtcgaag acctgtgccg
     3001 ccggttcccc ctctatccca agcggcgcaa atgacgtttt agacggtaaa tcaccgaaag
     3061 gaacgcgccc ggaaaaaccc atgcccaaaa agagactgat cgtcctcgac cacccgctca
     3121 ttcagcacaa gctcaccatg ctgcgcgacc gcaccaccaa ttcccgcgat ttccgcgacc
     3181 tgttggacga gatctccacg ctcatggcct acgaggtcac gcgccatctc cccacccagg
     3241 cccggacggt gaccaccccg ctcatgcggg cgcgggggaa gatgatcccc ggcaagatgc
     3301 tgggcgtggt ggtgatcctg cgggcggggc tcggcatggt ggacggcatc ctcaagctgg
     3361 tgccgggcgc caaggtcgga cacatcgggc tctatcgcga tccgcagacc atgcggccca
     3421 tcgaatattt ctgcaaattc ccgccggacg tgcccaagag gttcatgatt ttggtggacc
     3481 ccatgctggc cacgggttat tccgcctcgg ccgcgctgga catcctcaaa cgcgccggcg
     3541 cgcggaaggt ctccctcatg tgcctgctgt cctgcccgga agggctgcgc gtggtgcagc
     3601 gccgccatcc ggacgtgccc atctttacct gcgcggtgga caagaaactc aattcccacg
     3661 gctacatcct ccccggtctc ggggacgccg gcgaccgcct cttcggtacc cgctgacggg
     3721 catgtctcgg accggcgccg gtcttccctc ctaggaatcc gggatgctcc agatctacct
     3781 catcgccctg ttctgcgcgt tggccggcgc ggcgttcttc acggcgcgaa tcgccggcgg
     3841 gctcatcgcc tcgccgacgt tgaggacgcc ttccacgaat tggattcggc cgctgcggcc
     3901 acggcgcaac acgccgaggc aatgcggcgt ttcgcgaatc gcgtgggcgg gacagaccaa
     3961 g
//
LOCUS       DAOTFD010000230         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_16928_length_3953_cov_4.448692, whole genome shotgun sequence.
ACCESSION   DAOTFD010000230 DAOTFD010000000
VERSION     DAOTFD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16928_length_3953_cov_4.448692"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /gene="uvrB"
                     /locus_tag="P5079_10295"
     CDS             <1..1894
                     /gene="uvrB"
                     /locus_tag="P5079_10295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRY30409.1"
                     /translation="AVAQVNLPTLVISPNKILAAQLYAELKSFLPKNAVEYFISYYDY
                     YQPEAYVPQSDTYIEKDSSVNDHIDRLRLKATSTLLERRDVVVVASVSCIYGLGSPKE
                     YKEMCVSVEVGRAKSREELLRELVSIHYERNEVAFARGMFRVRGDTVEIFPAYLETGL
                     RVELDGDTVARLREINVLTGEVLREKERVFIYPARHFVTTGPTIERALGDIRRELEER
                     LAVLRQGGKLLEAQRLEQRTRFDMEMMKELGFCHGIENYSRHLAGRKPGERPACLLDY
                     FPKDFLVVLDESHVTVPQIGGMYEGDRSRKQTLVDYGFRLPSALDNRPLKFPEFEALV
                     PQTIYVSATPGPYELKKTAGVVAEQIIRPTGLVDPEVLLRPVQGQVEDLIDLVKERVN
                     RKERTLITTLTKRMAEDLSEYLSGKGLKVRYLHSEIEALRRVEILKDLRKGNFDVLVG
                     INLLREGLDLPEVSLVVVLDADKEGFLRSETTLIQVCGRAARNVNGQVVLYADTMTGS
                     MKRALDEMTRRRKKQVAYNEEHHITPKTVVKAIHELEEFQYAAKNAELPGLFRETGAR
                     YLTKEKLPGLIKDMETQMKEAADALDYELAAVIRDKIGELREMMVRPGRRGRVGRKPS
                     KGRRGN"
     gene            complement(1891..3159)
                     /locus_tag="P5079_10300"
     CDS             complement(1891..3159)
                     /locus_tag="P5079_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY30410.1"
                     /translation="MKKSRAAAPSSRAASLIERLRRENRELREWRRRLSLLLEYGKKI
                     SVKKELDSLLHLLVEESKRVLRADRSTVFLLDKQANELWARVASGDALIRVPANKGIV
                     GEAVSTGNVIIIPDAYADRRFNPEVDRSTGYRTRNLLTAPMRNNRGEIIGAFQVLNKT
                     RGNFDAEDAEILLIFAEQAATAVENAQLYDEVRAAARDSIFRLAAAAEYKDKDTRNHL
                     ERMSHYSAILAEEMGMPHNWVQNIQLASPMHDIGKLGVPDAILKKPGKLEPPERQEIM
                     KHPEYGAEILKNSDNELIQMSERVARSHHEKWDGTGYPLGLKGEQIPIEGRIVALADV
                     FDALTSRRCYKPAFSMQETLHIIREGSGKHFDPKCVDTFFRALPRLVKVMEDFMDEPT
                     AENAETPKEAPLNGEPPLPSETPLPKIRLV"
     gene            3247..>3953
                     /gene="nadC"
                     /locus_tag="P5079_10305"
     CDS             3247..>3953
                     /gene="nadC"
                     /locus_tag="P5079_10305"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRY30411.1"
                     /translation="MNNFRREIKAALREDRVWHDRTTRTFVPARLRASAVIVSKQAGV
                     LSGVRAAAETFRLRDPRCRTSILVHDGKTIRRGRAVLRVEGPLSSLLSAERTALNFVT
                     HLSGVATLTREFVRRLGRAGPQLLDTRKTLPGLRRLQRWAVVCGGGTNHRLHLAEAIL
                     IKENHIAAVRGKKALRAFFRNVKALRRRGMKVEMECHTQKDIVWALLAGADILLLDNF
                     PPRLLPKVVRWIRQFCV"
BASE COUNT          724 a         1084 c         1347 g          798 t
ORIGIN      
        1 cgccgtcgcg caggtgaacc tccccacgct ggttatctca cccaataaga tcctcgccgc
       61 ccagctttac gccgagctga aatccttcct gccaaaaaac gccgtggaat acttcatctc
      121 ctattacgac tattatcagc ccgaggctta cgtgccccag agcgacacct acatcgaaaa
      181 agattcgtcg gtcaacgacc acatcgaccg gttgcgcctg aaggccacct ccactctttt
      241 ggagcggcgc gacgtggtgg tggtggcgtc ggtgtcctgc atttacggat tggggagccc
      301 gaaagaatac aaggagatgt gcgtgagcgt cgaggtgggg cgcgccaaga gtcgcgagga
      361 gttgctccgg gaactggtga gcatccatta cgaacgcaac gaggtcgctt ttgcccgcgg
      421 gatgttccgt gtgagggggg acacggtgga gatattcccg gcctatctgg agacggggct
      481 gcgcgtggaa ctggacggcg atacggtggc tcggctgcgc gagatcaatg ttttgacggg
      541 cgaggtcctt cgagaaaaag agcgggtctt catttatccg gcgaggcatt tcgtgaccac
      601 gggtcccacc atcgagcgcg ctttggggga catccggcgc gaattggagg agcgactggc
      661 ggttcttcga caggggggga aactgttgga ggcccagcgg ctcgaacagc ggacccggtt
      721 cgatatggag atgatgaagg aactgggttt ttgccacggg attgagaatt attcacggca
      781 tctcgcggga cggaagcccg gcgaacgccc cgcctgtctc ctggattatt tcccgaagga
      841 tttcctggtc gttctcgacg agtcccatgt gacggtgcct cagatcggag ggatgtacga
      901 gggggaccgc tcccggaaac agaccctggt ggattatgga ttccgcctgc cgtcggcgct
      961 ggacaatcgg ccgctgaagt tccccgagtt cgaggcgttg gtcccgcaga ccatttatgt
     1021 ttcggccacg ccggggccgt atgagctgaa aaaaaccgcc ggcgtggtgg ccgaacagat
     1081 catccgcccg acggggctgg tggatccgga ggtgttgctg cggcccgtgc aggggcaggt
     1141 ggaggatttg atcgatttgg tgaaggaacg ggtgaaccgg aaggaacgca ccctcatcac
     1201 cacgctcacc aagcgcatgg cggaggacct gtcggaatat ttgtcgggca aggggctgaa
     1261 ggttcggtat ctccattcgg agatcgaggc gttgcggcgg gtcgagatat tgaaggacct
     1321 tcgcaagggg aacttcgacg tcctggtggg gatcaacttg ttgcgggaag gactggacct
     1381 gcccgaggtg tccctggtgg tcgttttgga cgccgacaag gagggttttc tgcggtccga
     1441 aaccacgttg atccaggtgt gcggccgcgc ggcgcgcaac gtgaacgggc aggtggtcct
     1501 ttacgccgac accatgaccg gttccatgaa acgcgcgctc gacgagatga cccgccggcg
     1561 gaagaaacag gtggcgtaca acgaggaaca tcacatcacg cccaaaacgg tggtcaaggc
     1621 catccacgag ctggaagaat ttcaatacgc cgccaaaaac gcggagttgc ccgggttgtt
     1681 ccgcgagacg ggggcgcgct atttgacgaa ggagaaactg cccggactca tcaaggacat
     1741 ggaaacgcag atgaaagagg cggccgacgc gctcgattac gaattggcgg cggtcattcg
     1801 ggacaagatc ggggaattgc gggaaatgat ggtccggccg gggcggcggg gcagggtggg
     1861 gcggaaaccc tcaaagggtc gtcgcgggaa ttagacgaga cgtattttcg ggagaggcgt
     1921 ttccgacggt aaggggggct ccccgttgag gggggcctct ttcggcgtct cggcgttttc
     1981 ggcggtgggc tcatccatga agtcctccat gactttgacg agccggggca gggcgcgaaa
     2041 gaacgtgtcc acgcatttcg ggtcgaaatg ttttcccgac ccttcgcgga tgatgtgcag
     2101 ggtttcctgc atggagaagg ccggtttgta acagcgccgt gaggtgagcg cgtcgaagac
     2161 gtcggccagg gccacgatcc gtccttcgat ggggatctgt tcccctttga gaccgagcgg
     2221 atagccggtg ccgtcccact tttcgtgatg ggaacgggcg acgcgttcgg acatctgaat
     2281 gagttcgttg tcggaattct tgaggatttc ggcgccgtat tccgggtgct tcatgatctc
     2341 ctggcgttcc gggggttcga gcttgccggg tttcttgagg atggcgtcgg gcacgcccag
     2401 cttgccgatg tcgtgcatgg ggctggccag ctggatgttc tgcacccagt tgtggggcat
     2461 gcccatttcc tcggcgagga tggcgctgta gtggctcatg cgttcgagat ggttgcgggt
     2521 gtccttgtcc ttgtattcgg cggcggccgc cagccggaag atgctgtccc tcgcggcggc
     2581 tcggacctcg tcgtagagtt gggcgttctc caccgcggtg gcggcctgtt cggcgaagat
     2641 gaggaggatc tccgcgtctt ccgcgtcgaa attcccccga gtcttgttca atacttgaaa
     2701 cgcgccgatg atctcgcccc ggttgttccg catgggcgcg gtgaggaggt tgcgggtgcg
     2761 gtagccggtg gacctgtcca cttccgggtt gaaccgtcgg tcggcgtagg cgtccgggat
     2821 gatgatgacg ttccccgtgc tgacggcttc gccgacgatc cctttgttgg ccggcacgcg
     2881 gatgagcgcg tcgccggagg ccacgcgggc ccagagttcg ttggcctgtt tgtccagaag
     2941 aaaaaccgtg gagcggtccg cccggagaac gcgtttgctt tcttcgacga ggagatggag
     3001 gagggagtcg agctctttct tgacggaaat ctttttcccg tattccagca ggagggagag
     3061 ccgccgccgc cattcgcgga gttcccggtt ctcgcgccgg agcctttcga tcaaagaggc
     3121 ggcgcgggag gagggcgcgg cggcgcggga ttttttcata acgcgcgtat cgtagcaatt
     3181 tttcggccga cgggcaaagc agtttccgat tgacagaggc gaaggggaaa gataaacttt
     3241 ccgccgatga ataatttccg acgagaaata aaagccgcct tgcgggagga ccgggtgtgg
     3301 cacgaccgca ccacccgcac cttcgtcccg gcgcggctga gggcttccgc cgtcatcgtt
     3361 tccaagcagg cgggggtgct gtccggcgtg cgcgcggcgg ccgaaacctt ccgcctccgc
     3421 gatcctcgct gccgcacgag catcctcgtt cacgacggaa aaacgatccg tcggggtcgg
     3481 gccgtcctgc gcgtggaggg gccgctctct tcgctccttt cggcggaacg caccgcgttg
     3541 aatttcgtca cgcatctctc cggcgtggcc accctgacgc gggaattcgt gcggcgcctc
     3601 ggccgggccg gtccgcagct tctcgatacg cgcaaaacgc ttcccggact gcggcgcctt
     3661 cagcggtggg ccgtggtctg cggcggagga acgaaccatc gcctgcattt ggcggaggcc
     3721 attctcatca aggaaaatca catcgccgcc gtccggggaa agaaagcctt gcgcgcgttt
     3781 tttcggaatg tgaaagcgct gaggcgtcga ggcatgaagg tggagatgga gtgtcacacg
     3841 caaaaggaca tcgtctgggc gctcctcgcc ggggccgata tccttttgct ggacaatttc
     3901 ccgccgcgcc tcttgccgaa agtggtgcgc tggatacggc aattctgcgt caa
//
LOCUS       DAOTFD010000231         3937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17037_length_3937_cov_4.830500, whole genome shotgun sequence.
ACCESSION   DAOTFD010000231 DAOTFD010000000
VERSION     DAOTFD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17037_length_3937_cov_4.830500"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="P5079_10310"
     CDS             complement(<1..1029)
                     /locus_tag="P5079_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY30412.1"
                     /translation="MEARPLPYFFRRPLLMVVAAAVFGAVVFRAWVPPRLPPDDAAHY
                     ADGEDAILRGTLASDPDFDGQKHRFVLRVRSVQKEGMRDPLPASGLVRVGVADAWFPA
                     GWGDSVALKGPFFRPSAASAPGGFDYRAFLQEKNIHSQMSVFPGGWRRVRRASAASPR
                     RWVGALRDRFFSAYGRRLSPHGAALMSGLVLGKKPASYPAVEDDFRRSGTYHLLVASG
                     SNVGFVIGLWFFVARWLLFLPRRAIWGSAVGWAFLYAGLAGMDPPVVRAALMVSLGIA
                     GYLLAREDRVEQAVGFSMGVFLLLRPRALFEAGFQMSYAATLGVVLAVPALEERLSGA
                     FRESGPRFW"
     gene            complement(1049..3250)
                     /locus_tag="P5079_10315"
     CDS             complement(1049..3250)
                     /locus_tag="P5079_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY30413.1"
                     /translation="MKIALVGIEARPFSPYLSLGYLAAFLMNDPALDGLIRLRLRQFC
                     LSAESPEDIAEAVLAEDPDVAAFTCYVWNVNVIGRVAHLVKRKKKSCRVVVGGPEAFP
                     RGPGYLLENPDVDAVIRDEGESTFAQYVRALLGSGDLKDVAGLYFRRDGRVEETAPRP
                     LLPDLSVVPSPILQGLFLYSQPTPQIVVEGARGCLYDCKFCSWQTQRIIRYFPEERLV
                     AELRHIVKNRLSPWIYLADANIFSDLPRGKRLIKEFIKVAEEEHYGTFSVEANPEDVD
                     AEVMEILGSSTAVEFQWGVQSVDPAVQRRNTRSFHLSKTAEILGRFRKNVPFASDKIQ
                     LMIGQAGDTLGTIRRSIDWCFSQNPRQIELHRLVYISGSGYARDPKAFPMEVDPQTRE
                     VVATDTMTREDFETARDWAIHLPTLQRLFIVRQALYVWGARQGQGAEEPYLRAYENFQ
                     KVLAEGGLDKETLWTFYRGLDYGHLLPWKIKESRPLLRKINAALRRFTGALSGDDELA
                     AYLKEMADSFDRESAWGAALADKFWEAVWTHAGRTMSPERVLLVGPLSTADAIIWAGW
                     AREVTAVDLSRDRYGQFHKLERTTRRNIRQRFVDSILGEDALRGLEGTYSTVVFVNAW
                     QPADADIFARWIKEMRGRGMKSDILLAAWASPGDPPRSPREWNETRIGLEKKYDLLSG
                     MPPPIDFPAVSSRALERSFVRAGLTMSPLADDMKTFFSAEGSRPVFFSGRL"
     gene            complement(3306..3881)
                     /locus_tag="P5079_10320"
     CDS             complement(3306..3881)
                     /locus_tag="P5079_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY30414.1"
                     /translation="METKTSGSPRIKRHTLIIKKALQAKFILLVMASVFFAVCLVGWE
                     IYYTFGRDIVRDLMDPGLYELFQQLSRVLVAKIAIYMAVVGIVAVFLSHKLAGPIYRF
                     EKSAHVLGGGDLTHRVHLRKGDELMDLQDEFNSMAEALHVRVAKDRQLAQRIAGQMQD
                     LTKGKDISPDLARRLKELETEVNHLTADFKV"
BASE COUNT          790 a         1338 c         1177 g          632 t
ORIGIN      
        1 ccaaaaacgc gggccgctct cccggaaagc cccggacaat ctttcttcca acgccggcac
       61 cgccagcacc acccccaagg tggcggcgta ggacatctga aaacccgcct cgaaaagcgc
      121 cctcggcctc aacaacaaaa aaacccccat ggaaaaaccc accgcctgtt ccacgcggtc
      181 ttcccgcgcc aacagatacc ccgcgatgcc gagggacacc atcagcgccg cgcgcaccac
      241 gggggggtcc atccccgcca gccccgcgta gaggaacgcc cagccgacag cgctgcccca
      301 gatcgcccgg cggggcagga acaacagcca acgcgccacg aagaaccaga gcccgatgac
      361 gaacccgacg ttggaccccg acgccaccag gaggtggtag gtgccgctcc gtcggaaatc
      421 gtcttccacg gccgggtagg aggccggttt tttccccaac accaatcccg acatcagcgc
      481 cgccccgtgc gggctcaagc gccggccgta ggccgagaag aaccggtccc gcaacgcccc
      541 cacccagcgc cgcggcgaag cggcggacgc ccgccgcacg cgccgccagc cgccggggaa
      601 aacgctcatt tgggaatgga tgttcttttc ctgaaggaag gcgcggtaat cgaacccgcc
      661 gggcgccgaa gccgccgacg gccggaagaa cggccccttg agggccaccg agtcccccca
      721 gcccgcgggg aaccacgcgt ccgccacgcc gacgcggacg agccccgacg cgggcaacgg
      781 gtcccgcatc ccctcctttt gcacggagcg aacccggagg acgaaccgat gtttttggcc
      841 gtcgaagtcg gggtcggacg ccagggtgcc gcgaaggatg gcgtcttcgc cgtcggcgta
      901 atgggcggca tcgtcgggag gaagccgggg cgggacccag gcgcgaaaaa ccaccgcgcc
      961 gaacacggcg gcggccacga ccatgaggag cggtcgacgg aaaaaatagg ggaggggccg
     1021 ggcttccacc gacgccgtgg cggcggacct agagccggcc ggaaaaaaac acggggcgtg
     1081 atccctcggc actgaaaaac gttttcatgt cgtccgccag cggagacatc gtcaaacccg
     1141 cccggacgaa cgacctctcc aacgcccgcg aggaaaccgc ggggaaatcg atgggcggcg
     1201 gcatgccgct caacaaatcg tattttttct ccagcccgat gcgggtttcg ttccattccc
     1261 gcggagaacg cggcgggtcg ccgggcgacg cccaggccgc caggaggatg tccgatttca
     1321 tcccccgtcc ccgcatctcc ttgatccagc gcgcaaagat atcggcgtcg gccggctgcc
     1381 aggcgttgac gaacacgacc gtcgaatagg tcccctccaa gccccggagg gcgtcctccc
     1441 ccaggatgga atcgacgaaa cgctgccgga tgttgcggcg agtcgtcctt tccaatttat
     1501 ggaattggcc gtagcggtcg cggctcaagt ccaccgccgt cacctcacgc gcccagcccg
     1561 cccagataat cgcatcggcc gtgctcagag gccccaccag caggacccgt tcgggggaca
     1621 tcgtccggcc ggcgtgcgtc cagaccgcct cccagaattt atcggccaag gccgcccccc
     1681 acgccgactc ccggtcgaac gaatccgcca tctccttcaa atacgcggcc agctcatcgt
     1741 ctccggagag ggcgccggtg aaccggcgca gcgccgcgtt gattttccga aggagcggac
     1801 ggctctcctt tatcttccat gggaggagat gcccgtaatc gagcccccgg taaaaagtcc
     1861 aaagggtctc cttgtccaac ccgccctcgg ccagaacttt ttgaaaattt tcgtacgcgc
     1921 ggagatacgg ctcttcggcc ccttggccct gacgggcccc ccacacgtac aacgcctgcc
     1981 gcacgatgaa aagcctctgg agggtgggca gatggatggc ccagtcccgc gcggtttcaa
     2041 agtcctcccg cgtcatggtg tccgtggcca cgacctcgcg ggtctgcggg tccacctcca
     2101 tggggaacgc tttcggatcc cgcgcgtagc ccgaaccgga gatatagacg agccggtgca
     2161 attcgatctg tcgcgggttc tgggaaaaac accagtctat ggagcgccgg atcgtcccga
     2221 gggtatctcc cgcctggccg atcatcaatt ggatcttatc gctggcgaac ggcacgtttt
     2281 tccggaacct cccgaggatt tccgccgtct tggacaggtg aaaactccgc gtgttcctcc
     2341 gctgcacggc cggatcgacg ctctgcaccc cccactgaaa ctccacggcc gtcgaagagc
     2401 ccaggatctc catcacttcc gcgtccacgt cttccgggtt cgcctcgacg gaaaaagtcc
     2461 cgtaatgttc ctcttccgcc acttttatga attcttttat cagccgcttg ccccgcggaa
     2521 ggtccgaaaa gatgttcgcg tccgccagat agatccacgg gctcaacctg tttttgacga
     2581 tgtgccgcaa ctccgccacg agccgctctt cggggaaata ccggatgatc cgctgcgtct
     2641 gccaactgca gaacttgcag tcataaaggc acccccgggc cccctccacg acgatctgcg
     2701 gcgtcggctg agaatacaaa aaaagaccct gcaagatggg ggacggcacg accgacagat
     2761 ccggcaggag cggccggggc gccgtctcct ccacgcgtcc gtcgcggcgg aaataaagcc
     2821 ccgcgacgtc cttcaagtct ccggaaccca aaagggccct cacgtactgc gcgaacgtcg
     2881 actccccctc gtcccggatc acggcgtcca cgtcggggtt ctccaacaga tagccgggcc
     2941 cccgaggaaa cgcctccggt ccgccgacca ccacccggca ggactttttc ttcctcttga
     3001 ccagatgggc cacccggccg atgacattga cgttccagac ataacacgtg aaagcggcca
     3061 cgtcgggatc ttccgcgagg acggcctccg cgatgtcttc cggactttcc gcgctgaggc
     3121 agaactgtcg gaggcggagc cggatcaaac cgtccagggc ggggtcgttc atcaggaaag
     3181 cggcgagata ccccaacgag aggtagggcg agaaaggcct ggcttcaatt ccgaccagcg
     3241 ctattttcat gggagttccc cgagggggcc gccgtcgagg acggcgggga ctgcggagtg
     3301 cgtggttata ccttgaaatc ggccgtgagg tggtttactt ccgtctcgag ctccttgaga
     3361 cggcgggcca ggtccgggga gatgtccttg cccttggtca ggtcctgcat ctggccggcg
     3421 atgcgctggg ccagttggcg gtccttggcg acgcgcacat ggagggcctc cgccatggag
     3481 ttgaattcgt cctgaagatc catgagttca tcgcccttgc gcaggtgcac ccgatgggtg
     3541 aggtcgccgc cgcccagcac gtgagccgat ttctcgaaac ggtagatcgg gccggccagc
     3601 ttgtgcgaca ggaacaccgc cacgatcccc accaccgcca tatagatggc gatcttggcg
     3661 acgaggaccc ggctgagctg ctgaaaaagc tcgtagaggc cgggatccat caggtcccgg
     3721 acgatgtcgc gcccgaacgt gtaataaatc tcccagccga ccaggcacac cgcaaagaaa
     3781 acggacgcca tcaccaggag gatgaacttg gcctggagcg cttttttgat gatgagcgta
     3841 tgccgcttga tgcggggaga gccggacgtt ttcgtttcca taggtccgtt agaaattaac
     3901 agaaaaaacg gcctttttaa actccccgcc cggcaaa
//
LOCUS       DAOTFD010000232         3930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17088_length_3930_cov_3.921548, whole genome shotgun sequence.
ACCESSION   DAOTFD010000232 DAOTFD010000000
VERSION     DAOTFD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17088_length_3930_cov_3.921548"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3930
                     /locus_tag="P5079_10325"
     CDS             <1..>3930
                     /locus_tag="P5079_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30415.1"
                     /translation="REMKAAIETEAKLAAHEAMLGEFSGVFGDRLMEARVELRQETLP
                     GGEVATVVEARVGDLAGIARERAGKWGLTEKDTVRLTKRGEDITMTFERTPAQFETSE
                     MGKKVMGEAVFGSVIGDAKMVLKYTVTVDVASGRSTVTADVRYEGLKGEAKDLAKAAG
                     YEGKTVRVEMKVDENGTVESASLKFDRIEGQHVDGFLKYLEENGKADEAKALSAKLKQ
                     MGLEGKIEALENVTIEISRRTASGEAVTGVFGGRVDVVVDAKALRAEVQQGLGSPIAL
                     VEGDKVVLTMDVATGAELRTEIRFSESASGVLNRVRNALKNREPKGPELRLLVLLEEI
                     AKNPAFADAKVSLVIDAKTRSASLFVKNVEGRYVNAERLLTKAGADRYAECVDIPKQI
                     EALTEQIKTEGEKAKTADAATAEMIGLENAARQAKVEYLKERFGELLQGRGEPSARQR
                     GLAQAAAKSELATSIKKIEQMRAAEVARGADARKDVLKSFDALLKQAKDMLRDGKMSF
                     ERAQEVMRLPLAEQAKKMLDLRDEISMAREQMKEGTPAEREAAEAMDAFIQVLTQEGY
                     ADGRARGRRESGALESAERLFDALDRAYGVRGENKKAEVKELLEKLRNAPDEAAAFDI
                     LDRLAFAMFGKKAVTELNVVERYALVSHSAFEYQRGNWERGGRAITFESFAAKFREMG
                     VKQMEMVFDGLLGRISALSTGAGKTMTYIMTMGMLNLREGRQGIKAELFTAREGETAQ
                     YFRDYTGLARTFGLKLENGGELYRTHDMEGLANAYEGSGGRLVIYDLQARGFVELMAK
                     TESKRLNAALEKVDVRIVDEADVAALSRVAFLLGSGDMVASKGLRAEVARLLGEIEKS
                     GLRAVETEIFEIIETAVAKEKGFDAAELGIYGKEKIELAKKIYEETGGKTYTVRQGGE
                     ILVSTKLMEDGKTTYRDQFLADMKGKKFEEGQVANALRAKFVVELKKGEWGVFDKGQM
                     TSIQDGVLQVGTSDTSQTYNVATVLMEIAAYKKAGLSERQIKEQHGLDAEHIKLSQGV
                     GESTLSQIFSRGGGKVLTMGGSATLDVAKQIAESIYGVKAVDIEGSSLKQYFDAQVKK
                     GQMDLRSRNFEAIMEETVKLMLEGEAGISRDRGFLVGAMEGRYLKEALVRTLERMFTA
                     EHGAELGKQIRDAAKKYGNLEAGGMFDAIVRELKLDQYKLKSDVGKQKLGGIEARLST
                     MSAGDLIKTLSEVFDGAYARDGRSMTEHIGDIARQNEGKSARELYEALTPLLSGEMFR
                     TVTDLVKVIDATLSAGVKEGEDASTYTKLIQGRFIFTNESGLRGMDY"
BASE COUNT          881 a          829 c         1595 g          625 t
ORIGIN      
        1 cgcgggagat gaaggcggcg atcgagacgg aggcgaagct ggcggcgcac gaggcgatgc
       61 tgggcgaatt ctccggagtg ttcggagacc ggctgatgga ggcgcgcgtg gagctgaggc
      121 aggagactct gccgggcggc gaagtggcga cggtggtgga ggcgcgggtg ggggatctgg
      181 cagggatcgc ccgggagcgc gcaggcaagt gggggctgac cgagaaggac acggtgcgtc
      241 tgacgaagcg cggggaagac atcacaatga cttttgagcg caccccggcg cagttcgaga
      301 catcggagat gggcaagaag gtgatgggcg aggcggtgtt cgggtcggtg atcggggacg
      361 cgaagatggt gctgaaatac acggtgacgg tggacgtggc gtccggccgg tcgacggtga
      421 cggcggatgt gagatacgaa gggttgaagg gggaagcgaa agatctggcc aaagcggcgg
      481 ggtatgaagg caagacggtg cgggtggaga tgaaggtgga cgaaaacggg acggtggaga
      541 gcgcctcgtt gaaattcgac cgtatcgaag gccaacacgt ggacgggttc ctgaaatatt
      601 tggaagagaa cgggaaagcc gacgaggcga aggcgctgtc ggccaagttg aagcagatgg
      661 ggctggaagg aaagatcgag gcgctggaga acgtgaccat cgagatatcg cgacgtacgg
      721 cgagtgggga agcggtgaca ggggtgttcg gcgggcgtgt ggatgtggtg gtggacgcca
      781 aggcgttgag ggcggaagtg cagcaggggc tgggatcgcc gatcgctttg gtggaaggcg
      841 acaaagtggt gttgacgatg gatgtggcga cgggggcgga gttgaggacg gaaatccggt
      901 tctccgagtc ggcttccggg gtattgaatc gggtgaggaa tgcgctgaag aatcgggagc
      961 cgaaaggtcc ggaactgcgg ctcctcgtgc tcttggaaga gatcgccaag aatcccgcct
     1021 ttgccgatgc caaggtgagc ctggtgatag atgccaagac ccgttcggcc tccctgttcg
     1081 tcaagaatgt ggaagggcgt tatgtgaacg cggagcgtct gttgaccaag gccggggcgg
     1141 accgatacgc cgaatgcgtg gacattccga aacagataga ggctctgacg gaacagatca
     1201 agacggaggg ggagaaggcg aagacggcgg atgcggccac ggcggagatg atcggattgg
     1261 agaacgcggc gcggcaggcg aaggtggaat acctgaaaga gcggttcggc gagttgctgc
     1321 aggggcgggg cgaaccttcg gcgaggcagc gtgggttggc gcaggcggcg gccaagtctg
     1381 agttggcgac gtccatcaag aaaatcgaac agatgcgtgc ggcggaggtg gcgcgggggg
     1441 cggatgcccg caaagacgtt ctgaaatcgt tcgacgcgct gctgaagcag gccaaggaca
     1501 tgttgagaga cgggaagatg agttttgaac gggcgcagga agtgatgcgg ttgccgttgg
     1561 cggaacaggc gaagaagatg ctggatttga gggatgagat atccatggct cgcgagcaga
     1621 tgaaggaggg gaccccggcg gagcgggaag cggctgaggc gatggatgcg ttcattcaag
     1681 tgctgacgca agagggatat gcggacggaa gggcgagagg gcggagagag agcggggcgc
     1741 tggagtcggc ggagcggctt ttcgatgcgt tggacagggc ctacggcgtg cgtggagaga
     1801 acaaaaaggc ggaagtgaag gagctgcttg aaaaattgcg gaacgccccg gatgaggcgg
     1861 cggcttttga catattggac cggttggcgt ttgcgatgtt cgggaagaaa gcggtgacgg
     1921 agctgaacgt ggtggaacgg tatgcgttgg tgtcgcacag cgcgttcgaa tatcagcgtg
     1981 ggaactggga gcgcggtggg cgggcgatca cgttcgagag ttttgccgcg aagttccgcg
     2041 agatgggcgt gaagcagatg gagatggtgt tcgacggcct gctggggcgg atcagcgcgt
     2101 tgtcgacggg cgcgggcaag accatgacct acatcatgac catgggtatg ctgaacctga
     2161 gggaagggcg tcaggggatc aaggcggaac tgttcacggc gcgggaaggg gagacggcgc
     2221 agtatttccg ggactacacg gggttggcga ggacgttcgg attgaaactg gagaacggtg
     2281 gagagcttta ccggacgcac gacatggagg ggctggccaa cgcctacgaa ggatcgggcg
     2341 ggcgcctggt gatctatgac ctgcaggcgc gcgggtttgt ggagctgatg gcgaagacgg
     2401 agtcgaagag gctgaacgcg gcgctggaga aggtggacgt gcggatcgtg gacgaggcgg
     2461 acgtggcggc gttgagccgg gtggcgttcc tgctgggcag cggggacatg gtggcgtcga
     2521 aggggttgag ggcggaggtg gcgcggctgc tgggcgagat agagaagtcg gggctgcggg
     2581 cggtggagac ggagatattc gagatcatcg agacggcggt tgcgaaagaa aaaggattcg
     2641 atgccgcgga gctggggatt tacgggaaag aaaagatcga actggccaag aagatttacg
     2701 aggagacggg cggcaagaca tacacggtcc ggcagggcgg ggagatcctg gtcagcacca
     2761 agctgatgga ggacggaaag accacctatc gcgaccagtt cctggcggac atgaagggga
     2821 agaaattcga ggaagggcag gtcgccaacg cgttgcgtgc gaaattcgtg gtggagttga
     2881 agaaaggcga atggggcgtt ttcgacaagg ggcagatgac ctccatccag gacggcgtgt
     2941 tgcaggtggg gacctccgac accagtcaga cctacaacgt ggcgacggtg ctgatggaga
     3001 tcgccgccta caagaaggcc gggttgtccg agaggcaaat caaggaacag cacgggctgg
     3061 acgcggaaca catcaagctg tcgcagggcg tgggcgaatc gacgctgtct cagatcttca
     3121 gtcgcggtgg cgggaaggtc ttaaccatgg gcggctcggc gacgttggac gtggccaaac
     3181 agatcgcgga atccatctac ggcgtgaagg cggtggacat cgaggggtcg tcgttgaaac
     3241 agtatttcga tgcccaggtg aagaaagggc agatggacct gcggagccgg aacttcgaag
     3301 cgattatgga agagacggtg aagttgatgt tggaggggga ggcggggata tcgagggacc
     3361 ggggtttctt ggtcggcgcc atggaagggc gttacctgaa agaggcgctg gtgcggacgc
     3421 tggagcggat gttcacggcg gagcatggcg cggagctggg caagcagatc cgtgacgcgg
     3481 caaagaaata cgggaacctg gaagcgggcg ggatgttcga cgccatcgtg cgcgagctga
     3541 aactggatca gtataaattg aaatcggacg tgggcaagca gaaactggga gggatcgaag
     3601 cccggttgtc cacgatgagc gcgggggatc tcatcaagac gctttccgag gtgttcgacg
     3661 gggcgtatgc ccgggacggc cgttcgatga cggaacacat cggcgacatc gcgcgccaga
     3721 acgaggggaa gagcgcccgg gaattgtatg aggcgttgac gccgctgctg tcgggcgaga
     3781 tgttccggac ggtgacggac ctggtgaagg tcatcgacgc gacgttgtcg gcgggcgtga
     3841 aagaaggtga ggacgcctcg acctacacga agctgatcca ggggcggttc atcttcacca
     3901 acgagagcgg tttgcgcggc atggactacc
//
LOCUS       DAOTFD010000233         3902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17239_length_3902_cov_4.541721, whole genome shotgun sequence.
ACCESSION   DAOTFD010000233 DAOTFD010000000
VERSION     DAOTFD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17239_length_3902_cov_4.541721"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="P5079_10330"
     CDS             <1..905
                     /locus_tag="P5079_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY30416.1"
                     /translation="SPSCPRCPFNRLKKTNCLYPLRGTRTGVLPKPGDLRRETGCRWE
                     CLASAEKIFRARRDRLAAAVVEPAVQAAAGMLVMPPGYLRGLERLCRRYGVLLIADEV
                     ATGFGRTGTMFAVQHEGVRPDLMCVSKSITGGTLPLAATLAREEIYRAFLGRYEEFKT
                     FFHGHTYTANPLACAAALANLRLYRTRRLLRRLTPRIRLLRSWLEGVASLPAVGSVRQ
                     IGLMAGVELVRKKNGMRPFPPELKMGQRVCRAARAGGLWIRPLSDIIVILPPLALPAP
                     MFRRMLGVIRESIRQTVPPAERPL"
     gene            902..2230
                     /locus_tag="P5079_10335"
     CDS             902..2230
                     /locus_tag="P5079_10335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY30417.1"
                     /translation="MKKKREAWLFFLLFSAYLVLCFALLAAPEQGTFLFPLLVGLILL
                     VAWLMGPGVSGLLILVASFVCLGLWNQWPEQQKIFLVLNVLQLWLIWYFLIRDDRQTI
                     QSRYEYETTVLAMEKGASELRKETEFYQRRGEELRGKAQQRQDLAACAKDLGAILDTT
                     EIQARLLRWTRKCFPGAQVFLSGLVEQDPLDAWILQRRQAVLCEDVSTHPVFSRARVE
                     PGTKALMVAPLWVENNIFGGLRVESSSPGRFGRDELRTLDALGTLGSLTLDNAVLYHR
                     IEQSAVRDGLTQLLTHRAFEERLGEEILRAGRYRYPLSLLMLDVDHFKRVNDTYGHPA
                     GDEVLRRVSARLAASGRPVDVVARYGGEEFAVLLVDLGKDQAVEFAENLRRGIESERF
                     ESGGYRYNVTISIGVAVYPDEAPTPPQLVRAADQRLYQAKTQGRNRVVWG"
     gene            2257..3504
                     /locus_tag="P5079_10340"
     CDS             2257..3504
                     /locus_tag="P5079_10340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY30418.1"
                     /translation="MIELAVWPLIPFLAALFGRSAMKPAVLWSVAVGGGCGWLLDGLL
                     RRPSPGVWISSGIAFAVGAAYVIVRGRQTYQLDAKTRTVIEKLKAEQAALEKTHAALR
                     QEYERYEREDDTAFQVYNLAKALTESFSWNTLVNRFSQALQKLAGSTDFLLYLSSDAG
                     ELEPKAQNGVWIRDSLPSPPMTQHPQWVPFGAETLLRVPLWQGNELLGLLWVRWAEPS
                     QPPIAEIETIFEYLLIGFKKAKLFARIESLSRLDGLTGVLRRQTFLDHLQVEWNRAKQ
                     FKTTFSLMLVDVDHFKRINDTYGHPAGDAVLARMGELLRMGVYETDYVGRYGGEEFGV
                     LLSRTDLAGVRRKAEMLRSRVAAELFPAGWEKISVTISVGLAHFPRDGQTVDGLFAAV
                     DRALYAAKNGGRNRVVDFSEVKG"
     gene            3501..>3902
                     /locus_tag="P5079_10345"
     CDS             3501..>3902
                     /locus_tag="P5079_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRY30419.1"
                     /translation="MIRRFGCFLGMVLWGVGVAHARPRIAFEKTDYDFGVLVEGSTAA
                     FSFPFKNTGGDPLEILAVAASCGCTAAAPADRVVGPGKKSEIRVVYDSHNRLGVTQKT
                     VRVQTNDPRDPVAQLFVRGMVVPVQHPTVRMG"
BASE COUNT          618 a         1124 c         1401 g          759 t
ORIGIN      
        1 tgagcccctc ttgtccgcgc tgcccattca accggctcaa gaagacgaac tgcttgtatc
       61 ctttgcgggg gacgagaacg ggggtccttc ccaaaccggg ggatcttcgc cgggagacgg
      121 gatgccggtg ggagtgtctg gcgagcgcgg aaaagatttt tcgcgctcgg cgcgaccggc
      181 tggcggccgc ggtggtggag ccggcggttc aggccgcggc cgggatgttg gtgatgccgc
      241 cgggttattt gcggggactg gaacgcctct gccgtcgata cggcgtgctg ttgatcgccg
      301 atgaggtggc gacggggttc ggccggacgg ggacgatgtt cgcggtgcag cacgaagggg
      361 ttcggcccga cctgatgtgc gtgtccaagt ccatcaccgg aggcacgctg ccgttggcgg
      421 ccacgctggc ccgggaggag atctaccggg cgttcctggg acggtatgag gagttcaaga
      481 ctttttttca cgggcacacc tacacggcca acccgttggc ctgtgccgcg gcgctggcca
      541 atctccgcct gtacaggacg cggcgcttgt tgaggagact gacgccgagg atccgcctgt
      601 tacgttcctg gctcgaaggg gtcgcgtctc tgccggcggt ggggagcgtt cggcagatcg
      661 gcttgatggc gggggtggaa ctggtgcgaa agaagaacgg gatgcggccg tttccgccgg
      721 aacttaaaat gggacagcgc gtctgccggg cggcgcgcgc cgggggctta tggatacgcc
      781 ccttgagtga tatcattgtc attctaccgc ccctggccct gccggcgcct atgttccggc
      841 ggatgctggg ggtcatccgg gaatccatcc ggcaaacggt gccgccggcg gaaaggccgt
      901 tgtgaagaaa aaacgcgaag cctggctgtt cttccttttg ttttcggcgt accttgtgct
      961 gtgtttcgcc ctcctggcgg cgccggaaca ggggacgttc ctgtttcccc tgctggtggg
     1021 attgatcctg ctggtggcgt ggctcatggg gcccggcgtt tcggggcttc tcatcctggt
     1081 ggcgtcgttc gtctgtctcg gcctttggaa ccaatggccg gaacagcaga agatattcct
     1141 cgttctgaac gtgctgcagt tgtggctcat ctggtatttc ctgatccggg acgaccgcca
     1201 gaccatccag tcgcgctacg aatacgagac caccgtcctc gccatggaga aaggggcctc
     1261 cgagttgcgg aaggagacgg aattctatca gcgtcgcggg gaggagttgc gcgggaaggc
     1321 gcaacagcgg caggatttgg cggcctgcgc caaggacctc ggcgccattt tggacaccac
     1381 cgagatccag gcgcggctgt tgcgttggac gcggaaatgt tttcccggcg cgcaggtttt
     1441 tctctcgggg ctggtggaac aggacccgtt ggacgcgtgg atactgcagc gccggcaggc
     1501 cgttttgtgc gaggatgtct cgacccaccc cgttttttcc cgcgcgcgcg tggagcccgg
     1561 aaccaaggcg ctgatggtgg cgccgttgtg ggtggagaat aatattttcg gggggctgcg
     1621 ggtggaaagt tcctcgcccg gccggttcgg ccgggacgaa ctgaggacgc tcgacgcgtt
     1681 gggaacgctg ggttccctga cgctcgacaa cgcggtgctt taccaccgca tcgaacaatc
     1741 ggccgtgcgg gacgggctga cgcagcttct gacccaccgg gccttcgagg aacggctggg
     1801 ggaggaaatc ctccgcgccg gccggtaccg gtacccgttg agtctgttga tgctggacgt
     1861 ggaccacttc aagcgggtca acgacaccta cggtcatccg gcgggcgatg aggtgttgcg
     1921 gcgggtgtcg gcgcgtttgg cggcgtccgg ccggccggtg gacgtggtgg cgcggtacgg
     1981 cggcgaggag ttcgccgtgt tgttggtgga cctcggcaag gaccaggccg tggagttcgc
     2041 ggaaaacctc cggcgcggca tcgagtcgga acgtttcgag tccgggggat accggtacaa
     2101 cgtcacgatc agcattgggg tggcggtcta tccggacgag gctcccacgc cgccgcagtt
     2161 ggtgcgcgcc gccgaccagc ggctgtatca ggcgaagacg caaggccgga accgcgtggt
     2221 ctgggggtaa ccggcgatgg attttcggag gggcccgtga tcgagttggc ggtttggccg
     2281 ttgattccgt tcctggcggc gctgttcggc cggtcggcga tgaaaccggc ggtgttgtgg
     2341 tcggtggcgg tggggggcgg ctgcggctgg ctcctggacg gccttttgcg ccgtccctcc
     2401 ccgggggtct ggatttcgag cggcatcgct ttcgcggtgg gggccgccta tgtgatcgtt
     2461 cggggacggc agacctacca gctcgacgcc aagacccgga ccgtcatcga aaaactgaag
     2521 gccgaacaag ccgccctcga gaagacccac gccgcgctgc ggcaggaata cgaacgatac
     2581 gagagggagg acgacaccgc ttttcaggtc tacaatctgg ccaaggcgct gacggaatcg
     2641 ttttcctgga acaccctggt gaaccgtttc tcgcaggctc tccagaaatt ggccggttcc
     2701 acggacttct tgttgtattt gtcctcggac gcgggggaat tggaacccaa agcgcagaac
     2761 ggggtatgga tccgcgacag cctgccctcc ccgcccatga cgcagcatcc gcagtgggtg
     2821 ccgttcggcg cggagaccct gttgcgggtg ccgctgtggc agggcaacga actcctcggc
     2881 ctgttgtggg tccgttgggc cgaaccgtcc cagccgccca tcgccgagat cgagacgatc
     2941 ttcgaatacc tgttgatcgg cttcaaaaaa gccaaactct tcgcgcggat cgaatcgctg
     3001 tcgcgtcttg acgggctcac cggcgttctt cggcggcaga cgttcctcga ccacctgcag
     3061 gtggaatgga accgcgccaa acagttcaag acgacgttca gcctgatgct ggtggacgtg
     3121 gaccatttca agaggatcaa cgacacctac ggacatcccg ccggggacgc ggtgttggcg
     3181 cggatggggg aactgctgcg gatgggcgtg tatgagacgg actacgtggg ccggtacggc
     3241 ggcgaggagt tcggggtatt gctttcgcgg acggacctgg ccggggttcg gcgcaaggcg
     3301 gagatgttgc ggtcgcgcgt ggccgccgag ctgttcccgg ccggctggga gaagatctcc
     3361 gtgacgatca gcgtggggtt ggcgcatttc ccgcgcgacg ggcagaccgt ggacggcctt
     3421 tttgccgccg tggaccgggc gctctatgcc gccaagaacg gcggccggaa ccgcgtggtg
     3481 gatttttcgg aggtcaaagg atgatccggc gtttcgggtg cttcttgggg atggtcctgt
     3541 ggggggtcgg ggtggcccac gcccgcccgc gcatcgcttt tgagaagacg gattacgatt
     3601 tcggcgtgct ggtcgaagga tccacggccg cgttttcatt cccgtttaaa aacaccggcg
     3661 gcgatccgct ggagatcctc gccgtggccg cttcctgcgg ctgcaccgcg gcggcgccgg
     3721 cggaccgcgt ggtggggccc ggcaagaaga gcgagatccg cgtggtttac gattcccata
     3781 accggctcgg cgtgacccaa aagaccgtgc gcgtccagac caacgatccc cgagacccgg
     3841 tcgcccaatt gttcgtccgc gggatggtcg tccccgttca gcatccgacg gtgcgcatgg
     3901 gg
//
LOCUS       DAOTFD010000234         3899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17263_length_3899_cov_3.406087, whole genome shotgun sequence.
ACCESSION   DAOTFD010000234 DAOTFD010000000
VERSION     DAOTFD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17263_length_3899_cov_3.406087"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1328
                     /locus_tag="P5079_10350"
     CDS             84..1328
                     /locus_tag="P5079_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HRY30420.1"
                     /translation="MKKILSLSIAGLLSLVLAVDPVRAVPNRITYQGRLSKSGVAVSG
                     PHIFVVRLLSSSGGPLWSSGNIPATLPATGDFSFTLEPIGVDWPNDDPRMEITVDGEK
                     MSPTEGFSATPYAFVAQQVVDGSVSAQKLTAGAVTNEKVAANAAIDPSKIALSGAVTL
                     ADWRSGANPAKIDANVIQGTVPVSSNQIDGTALVAHSTFTQVVEPTYPVKPLIIKPQS
                     STNVDILELQKENGGTAMLVKNTGDVEIAGRVSDATGFLMPVGSIVAYAGNAAPAGWR
                     LCDGAAVSRTTYATLFTAIGTSYGGGDGSTTFNLPDLRGRVAVGRDNMGGTTANRVTA
                     ASGMAGTTLGAAGGTETTTLSLAQIPPHDHGSGYLGGQNLGAGAINYVCYSNGGISIS
                     QGSGQAHQNMPPSLIVNHIIKY"
     gene            1436..3043
                     /locus_tag="P5079_10355"
     CDS             1436..3043
                     /locus_tag="P5079_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRY30421.1"
                     /translation="MHALPPQIPASVIALLLLFAPVAGLHADDAPPAGGARIRKIHIW
                     HKDIFDTAKPTENHFPYRLVNALHISTKNTAIKRELLLREGDVYNPQLKKESEIALRK
                     ILRLRKAKITTIPIDKNQVDLWVEVQETWSTEPILSFSGVGDRLSGKAGIKERNFLGW
                     GKDANFLYKKSMGRITRSYSYTDPNLWGSRLRLEVDYDERDEGLGKHFLVERPYYASI
                     TPWAGSANYRSNEEENRVFDNGNEVDRIFHENHDMSVSHSHSFGSTTRRIRRVGVGYQ
                     RIEDRQSRTSPIRTVLKDKNYDIANLKIHLERIKYLTADHINLYDRDEDYELGPSLSL
                     TAGRSDKWGKTSEPATFLKIDTFAGQSHGVSRFTLATGKLDGRYEDGDWRDTHARLDV
                     SHYNHFEPRQTIVAHFRAEHLTNPDPSSQILLGGDRGLRGYQINQFAGNKLLLGNLEN
                     RFFLVDDLWAVVGLGSVIFIDAGYVWDMNQVINFRDLKTDVGAGLRFHISRSSFGNVL
                     RLDVAYAVNRVPGESRVVITFGSSQAF"
     gene            3080..3640
                     /locus_tag="P5079_10360"
     CDS             3080..3640
                     /locus_tag="P5079_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30422.1"
                     /translation="MLRPNYTVIDTPDALSLLWAPPALPRTYFALGIAVLLCGLAVIF
                     FRKRIASELNEQLALEWLKERLFLWTGTAISAAGLFISAISLPQDPFIRWDITVTELR
                     VESLNGKIAIPWDAVKSVRLDEREQDLDKASLIVTDKGGKEGWLVLKWTLTPHRAKII
                     NALAARIPDRMKPVLENKDYMAKISG"
     gene            complement(3630..>3899)
                     /locus_tag="P5079_10365"
     CDS             complement(3630..>3899)
                     /locus_tag="P5079_10365"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HRY30423.1"
                     /translation="GHLLLLWATVAVFVSGYYCPLTVMETSLRVHYDPSAAYAEGFVA
                     GWLSRFLSWDLSRVQVGGATTLWAFFWTGIYVAMWRFEAKRKISR"
BASE COUNT          857 a         1310 c         1059 g          673 t
ORIGIN      
        1 aagcgcgcca agcggtcgaa cgcctgactc gccccgacgc ggccgaataa attttcaagt
       61 tttcccgaaa aggagatccc ttcatgaaga aaatattgtc cctgtcgatc gccggcctcc
      121 tctcccttgt cttggccgtc gatcccgtcc gtgcggtccc gaaccgcatc acctatcaag
      181 gccgcctcag caagtccggc gtggccgtga gcggacccca cattttcgtc gtccgtcttc
      241 tctcctcctc cggcgggccc ctttggagca gcgggaacat ccccgccacg ctgccggcca
      301 ccggtgattt cagtttcact ctcgaaccca tcggcgtgga ttggccgaac gacgatcccc
      361 ggatggaaat caccgtggac ggcgaaaaaa tgagcccgac ggaaggtttt tcagccaccc
      421 cgtatgcctt cgtggcccag caagtggtgg acggttcggt ttccgcacag aaactcacgg
      481 cgggggccgt caccaacgaa aaagtggcgg cgaacgcggc catcgatcct tccaagatcg
      541 cgctttccgg tgcggtcacc ttggccgact ggcggagcgg cgccaatccc gccaagatcg
      601 acgcgaacgt catccagggc acggtccccg tttccagcaa tcaaatcgac ggaacagcgc
      661 tggtcgccca ttccactttt acgcaagtcg tcgaacccac gtaccccgtc aaaccgctca
      721 tcatcaaacc gcaaagctcc accaacgtgg acatcctcga actgcaaaag gaaaacggcg
      781 gcacggcgat gttggtgaaa aacacggggg acgtggagat cgccggacga gtctcggacg
      841 ccaccgggtt cctcatgccc gtcggcagca tcgtggccta cgcggggaac gcggcgccgg
      901 cgggctggcg gctgtgcgac ggcgcggccg tgtcccgcac cacgtacgcc acgcttttca
      961 ccgccatcgg cacctcctac gggggcggag acggcagtac gacgttcaat ctgcccgacc
     1021 tgcgcggacg cgtggccgtc ggtagggaca acatgggcgg gacgacggcg aaccgggtca
     1081 ccgccgcctc cggcatggcc gggaccaccc tgggggccgc cggcggaacc gaaacgacga
     1141 cactctccct cgcccaaata ccgccccacg atcacggcag cggctattta gggggtcaaa
     1201 atctgggagc gggagcgatc aattacgtct gttacagcaa cggaggaata agcatttccc
     1261 aaggcagcgg gcaggcccat cagaacatgc cgccttctct catcgtcaac cacatcatca
     1321 aatactgacc gaaagaaaac tctcctcttt ccgatcctct ttcgtcccga acggagggcc
     1381 tccgctcctt ctccccctcg aatccgatat aatagtgccc cccatgaaaa cgtccgtgca
     1441 cgcacttccc ccgcagatcc cagcctccgt catcgccctc cttcttttat tcgcccccgt
     1501 tgccggattg cacgccgacg acgccccccc cgcgggcggc gcgcggatac gaaaaatcca
     1561 catttggcac aaggacatct tcgacacggc caaacccacg gagaatcact tcccctaccg
     1621 gctggtcaac gccctccaca tatcgaccaa gaacaccgcc atcaaacgcg aactcctcct
     1681 gcgggagggc gacgtctaca atccccagct caaaaaagag tccgaaatcg ccctccggaa
     1741 aatcctccgt ttgcgcaaag ccaaaatcac gaccatcccc atcgacaaaa accaggtgga
     1801 cctttgggtg gaggtgcagg agacttggag cacggaaccg atcctgagtt tttccggcgt
     1861 gggagaccgg ctttccggca aggcgggaat aaaggaacga aatttcctcg gctgggggaa
     1921 agacgccaac ttcctctaca aaaaaagcat ggggcgcatc acccgctcct actcctacac
     1981 cgatccgaac ctctggggaa gccgcctgcg cctggaggtg gattacgacg aaagggacga
     2041 ggggctgggc aaacacttcc tggtggaaag gccctactat gcctccatca ccccgtgggc
     2101 gggctccgcc aactaccgct ccaacgagga ggaaaatcgg gtcttcgaca acgggaacga
     2161 ggtggaccgg attttccatg aaaatcacga catgtccgtc agccattccc attccttcgg
     2221 ttccaccacg cgccgcatcc gccgggtcgg cgtgggctat caacggatcg aggaccggca
     2281 atcccgcacg tcccccatac gcacggtcct caaagacaag aactacgaca tcgccaatct
     2341 gaaaatccat ctggaacgga tcaaatatct caccgccgac cacatcaacc tctatgaccg
     2401 ggatgaagac tacgagctgg gcccctccct ctccctgacc gcggggcggt cggacaaatg
     2461 gggcaaaacg tcggagccgg cgaccttcct gaagatcgat acgttcgccg gacaatccca
     2521 cggggtctcc cgtttcaccc tggccacagg aaaactggac ggccgctacg aagacgggga
     2581 ctggagagac acccacgcgc gactggatgt ctcccactac aatcacttcg aaccccgcca
     2641 aaccatcgtc gcccatttcc gcgccgaaca tctgaccaac cccgacccct cctcccaaat
     2701 cctgctgggc ggcgaccggg gcctccgcgg ctatcagatc aaccagttcg ccgggaacaa
     2761 actcctcctg gggaacctgg aaaaccgctt cttcctggtg gacgacctct gggcggtggt
     2821 ggggctggga agtgtgattt ttatcgacgc aggatacgtc tgggacatga accaggtcat
     2881 caacttccgg gacctcaaaa cggacgtcgg cgcggggctc cgcttccaca tctcacgttc
     2941 gagtttcggc aacgtcctcc ggctggatgt ggcctacgcg gtgaaccgcg tgcccgggga
     3001 aagccgggtt gtgatcactt tcggttccag ccaggccttt tgaaatgcga atacgctata
     3061 atgaccttca aggattccca tgctgagacc gaattacacc gtcatcgata cgccggacgc
     3121 gctgtccctc ctctgggcgc cgccggccct gccgcggacc tattttgccc tggggatcgc
     3181 cgtcctcctt tgcggactgg ccgtcatttt cttccgcaag cgcatcgcct cggaactcaa
     3241 cgaacagctg gccctggaat ggctgaaaga acggctcttc ctctggacag gcacagccat
     3301 cagcgcggcc ggcctgttca tctccgccat ttcccttccg caggacccgt tcatccgctg
     3361 ggacatcacc gtcacggaac tccgggtgga gtccctcaac ggaaagatcg ccatcccctg
     3421 ggatgcggtc aagtcggtcc ggctggacga acgggagcag gatttagaca aggcgtcgtt
     3481 gatcgtgacg gacaagggag ggaaggaagg gtggctggtg ttgaaatgga cgttgacgcc
     3541 gcaccgcgcc aaaatcatca acgctttggc cgcgcggatc ccggaccgga tgaagcccgt
     3601 tttggagaac aaagactaca tggcgaaaat cagcggctga tctttcgttt cgcctcgaac
     3661 cgccacatcg cgacgtaaat tccggtccag aaaaaagccc acagcgttgt cgccccgccc
     3721 acctggacgc gggacaaatc ccacgacagg aatcgactca accacccggc gacgaaacct
     3781 tccgcatagg ccgcggaggg atcgtagtgc acccgcaggc tggtctccat caccgtcagc
     3841 gggcaataat accccgaaac gaacaccgcc accgtggccc acagcaacag gagatgtcc
//
LOCUS       DAOTFD010000235         3885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17340_length_3885_cov_3.453003, whole genome shotgun sequence.
ACCESSION   DAOTFD010000235 DAOTFD010000000
VERSION     DAOTFD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17340_length_3885_cov_3.453003"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3885
                     /locus_tag="P5079_10370"
     CDS             <1..>3885
                     /locus_tag="P5079_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30424.1"
                     /translation="TGLLKSGEAAAAAGRGESIAIDSFGNVTFTETKQTYTAVRNQAK
                     LDTATTSSFTESVDGSTTTTDPYNTKYEYDEAGRLVRAYLTGEIHRGESGRPYFIHSV
                     TLDAYGNVTHSYSNQEFIILAGQAKLDTVTAESVSSSVDGSVTVTDPYVTSYSYYEDS
                     PENRALRRVGNLSAAALVGLVHSTTTDAYGNISQSWTKQVFEVIAGQAKLVETETSSA
                     ADSVDGAHSETKPYVTKYEYDARGLLARVYLAGQTYAAPSTETDGQGYFIYSRTEDAF
                     GNVSESWSNQTFEVVAGQAKMSSVTSEALGTTVDGSRTVTKPYTIEYRYVAWQDGLAS
                     RTVGHLAWVTPEVNVESVATDSFSNVTNSTAIQRFEVLASIGQAKMMSVTSSSVAESV
                     DGTTTTTDPYLMEYHYDPATGLLERVNLAGPTQEVAGRTYFIHSRSEDSFGNVTDSWS
                     NQIYVVIAGQAKMVSVTTTSHAVSVDGTLTDTDPYTIAYAYDGEGVSPAEMRERVRQD
                     REAGRPLTIGHLTDVGDPRVVSISTDFFGNVTMTQSEQDFEVHAPTGQAKLMFVATAS
                     VSMGVDGATTITDRYVTQYHYENTGLLVSANLTGPVSGARSGNAYYIHATTEDAFGNV
                     THSWSNQVFDVVIGQAKLTAVTSESLSFGLDGTTTVTSPYTIAYRYKSLEEGRASRTA
                     GHMKWVTPEVNVHSQTTDPFGNITSSLAIQHFEVMASIGQAKMTSVTSSSIMESVDGS
                     VSVTQPYTTYYVYDAVGRLVSATVGATAVSSAPSAFMGRLLSLGVSRATLPGLLGLDG
                     TTTLFEQLGDGIIRFGVSTEEGDLAGLIRPGQAAQLANGVLSETYYIAVEGKSLKKQI
                     LLTTEYDQWGKVAISGSANSTYDAGDGNVLMSRTLQTYRFEDGRLYLDREKIFAGSLS
                     ETGEMTVTDPFDLLYLYTETGDLFSVKVKGDYVAPNGESYAIHTVTVDAFGNVTESWS
                     NQIYAVIGGQAKLTSVTSYSVSTGIDGAVTVTEPYTLRYQYNAQGLDPRGLEYKKFAQ
                     ARTVGHLNGVSDPTVRSTTQDIFGNVTLTVTTQDFEVHPATNQAKLMSVTTTVSTTQS
                     VDGSITTLQSPYAVHYTYDGRTGLLAHAEMDGRIHTQTIDAFGNVAQTYTQQTFIVIA
                     GQAKVKSATTSSNSYSVDGSYTETEPYTITYSYAGEELGKNPSRADIQDWVVKHGPTF
                     GHLRGVSQPNISSQTTDAFGNYTRSETLQVYVVVAGQAKLKSTVTKSETWGVDGTYSV
                     AEPYATTYHYMGEEEPAEFIGLLEGVDPVTIVS"
BASE COUNT          816 a         1113 c         1151 g          805 t
ORIGIN      
        1 accggcctgc ttaagtccgg agaggcggcg gccgccgcgg ggcgtgggga atccatcgcc
       61 atcgacagtt ttgggaacgt gaccttcacg gaaacgaagc agacgtatac ggcggtgcgc
      121 aatcaggcca aattggatac cgccacgacg tcctcgttta cggaatccgt cgacggttcg
      181 accaccacca ccgatcctta taacaccaaa tatgagtacg acgaggcggg gcggctggtg
      241 agggcgtatt tgacggggga aatccatcgc ggggagtcgg gtcggccgta tttcatccat
      301 tccgtcacgc tggatgccta cggcaacgtg acccattcct attccaacca ggagttcatc
      361 atcctggccg gccaggcgaa actcgacacc gtgaccgcgg aatcggtgag ttcttccgtg
      421 gatggttccg tcaccgtgac ggacccgtac gttacgtcgt attcctacta tgaagattcg
      481 cccgaaaacc gcgcattgcg gcgggtgggg aacctttccg cggcggccct cgtgggattg
      541 gtccattcca ccaccaccga tgcctacggc aacatctccc agtcttggac caagcaggtg
      601 ttcgaagtca tcgccggtca ggccaagctg gtggagacgg aaacatcttc cgcggcggat
      661 tccgtggacg gggcccacag cgagacaaaa ccttacgtca ctaaatacga atacgacgcc
      721 cgtgggttgt tggcgcgggt ctatctggcc ggtcaaacat atgcggctcc atcgaccgaa
      781 accgacggtc agggatattt catctattcc agaacggaag acgccttcgg caatgtgtcg
      841 gagtcatggt ccaatcagac tttcgaggtc gttgcaggcc aggccaaaat gtcctccgtc
      901 acttcggagg cgttggggac gaccgttgac ggttcccgca cggtcacaaa accctacacc
      961 atcgagtatc gctacgttgc ctggcaggac ggtctggctt cgcgcacggt ggggcatttg
     1021 gcgtgggtga ccccggaggt caatgtcgaa tccgtggcca ccgattcgtt ttccaatgtg
     1081 acgaattcca ccgcgattca gcgtttcgaa gtgctggcgt ccatcgggca ggcgaagatg
     1141 atgtccgtga cctcgtcttc cgtcgctgag agtgtggacg gaaccacgac caccaccgac
     1201 ccttacctta tggaatacca ttacgatccg gccacgggac tcctggaacg cgtgaatttg
     1261 gcggggccga cccaggaggt ggcgggccgg acgtatttca tccattcccg atcggaagat
     1321 tccttcggca acgtcacgga ttcgtggtcg aatcagattt acgtcgtcat cgccggccag
     1381 gccaagatgg tttccgtcac gacgacgtca cacgccgttt ccgtggacgg aacgctcacc
     1441 gacaccgacc cctacaccat cgcctatgcg tatgatgggg agggggtttc gccggcggag
     1501 atgcgggaac gggtgcgtca ggaccgtgag gccgggaggc cgctcaccat cggccatctg
     1561 acggacgtcg gcgatccgcg ggtcgtttcc atcagcacgg atttcttcgg caacgtgacg
     1621 atgactcagt cggagcagga ttttgaagtc catgccccca ccggccaggc caaactgatg
     1681 tttgtcgcca ccgcctccgt ttcgatggga gtggatggcg ccaccaccat cacggatcga
     1741 tatgtcactc agtatcatta cgaaaacacg gggctcctgg tttccgccaa tttgacgggg
     1801 ccggtctcgg gggcgcgttc cggcaacgcc tattacatcc acgcgacgac ggaagatgct
     1861 ttcggcaatg tgacgcacag ttggtcgaat caggttttcg acgttgtcat cgggcaggcc
     1921 aagttgacgg cggtcaccag cgaatccctt tcgttcggcc tggatgggac cacgacggtg
     1981 acgtccccct acaccatcgc ttatcgatac aagagccttg aagagggccg tgccagccgc
     2041 accgcggggc atatgaaatg ggttaccccg gaagtcaacg tccactccca aaccaccgat
     2101 cctttcggca atatcacctc ctctctggcc attcaacatt tcgaggtcat ggcatccatc
     2161 gggcaggcga agatgacgtc cgtgacctcc tcgtccatca tggagtccgt ggatggctcc
     2221 gtttctgtca cgcagccgta taccacttat tatgtttacg atgccgtggg gcgtcttgtt
     2281 tcggcgacgg tgggggcgac ggcggtttct tccgctcctt ccgctttcat ggggcgactg
     2341 ctgagtctgg gtgtttcgcg ggcgaccttg cccggacttc tgggattgga tgggacgacc
     2401 acccttttcg aacagttggg ggatgggatc atccggttcg gtgtcagcac ggaagagggg
     2461 gacttggccg gccttattcg tcccggccag gcggctcagt tggccaacgg ggtgttgtcg
     2521 gaaacctatt atatcgccgt tgaagggaaa tccctgaaaa aacagatatt gctgacaacg
     2581 gaatacgacc agtggggaaa ggtggcgatc agcggttcgg caaacagcac ctacgacgcc
     2641 ggcgacggga atgttctcat gtcccgtacc ctgcagacct atcggttcga ggacggcagg
     2701 ctttatctcg accgggaaaa aatattcgcg ggaagcttgt ccgaaaccgg ggagatgacg
     2761 gtgacggacc cctttgacct gctgtatttg tacacggaga cgggggatct gttcagcgtc
     2821 aaagtaaagg gggattacgt cgctccgaac ggcgagtcct acgccattca tacggtgacg
     2881 gtcgatgctt tcggcaatgt gacggaatcg tggtccaatc agatatacgc ggtcatcggc
     2941 gggcaggcca agttgaccag tgtgacgtcg tattctgttt ccaccgggat tgacggcgca
     3001 gtcaccgtca cggaaccgta tactctccgg tatcaataca acgcccaggg gcttgatccg
     3061 cgcggtcttg agtataaaaa attcgcccag gccaggacgg tcgggcatct gaacggggtc
     3121 agcgatccga cggtgagatc cacaacccaa gacatcttcg gcaacgtcac cctgacggtg
     3181 accacgcagg atttcgaagt gcatccggcc accaatcagg cgaaattgat gagcgtgacc
     3241 accaccgttt cgaccaccca gtccgtggac ggttccatca ccaccttgca gtctccctat
     3301 gccgtccatt acacctacga cggtcggacg ggcttgttgg cgcatgccga gatggacggt
     3361 cggatccata cgcagaccat cgatgccttc ggcaacgtgg cccaaaccta cacgcaacag
     3421 acctttatcg tcatcgccgg ccaggcgaaa gtgaaaagcg cgacgacgtc ctcgaactcc
     3481 tattccgtcg acggttctta tacggagacc gagccctaca cgatcaccta ttcctatgcg
     3541 ggtgaagagt tggggaaaaa tccaagtcgg gcggatattc aagattgggt tgtgaagcac
     3601 ggcccaacgt tcggtcattt gaggggggtt tcgcagccga atatctcctc gcagaccacc
     3661 gatgcgttcg gtaattacac ccgcagcgaa acgttgcagg tctatgttgt tgtcgccggc
     3721 caggcgaaat tgaaatccac cgtgacgaaa tcggaaacat ggggagtgga tgggacttat
     3781 tcggtggccg agccttatgc caccacgtac cattacatgg gggaggagga accggcggaa
     3841 ttcatcggtc tccttgaggg ggtggacccg gtgaccatcg tctcg
//
LOCUS       DAOTFD010000236         3825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17720_length_3825_cov_4.505570, whole genome shotgun sequence.
ACCESSION   DAOTFD010000236 DAOTFD010000000
VERSION     DAOTFD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17720_length_3825_cov_4.505570"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /gene="hisD"
                     /locus_tag="P5079_10375"
     CDS             <1..673
                     /gene="hisD"
                     /locus_tag="P5079_10375"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRY30425.1"
                     /translation="AYGTRTIPKVDLIVGPGGAWVTEAKRQVFGEVGIDLIAGPSEVA
                     VLADRTTSPRFAAADLMAQTEHDPLARAVLLSPDARTIAAVKGLVEKRFRPQCSFVKT
                     KNWADAVAKANALAPEHLSLLVKKPERLLNGVRNAGAIFLGPWSPVAAGDYWAGPSHV
                     LPTGRAARFMSGLSVQTFLKRSSIIRLSEPLMRKKARTIARLAEAEGLVYHAASLKAR
                     LNRSR"
     gene            690..1319
                     /gene="hisB"
                     /locus_tag="P5079_10380"
     CDS             690..1319
                     /gene="hisB"
                     /locus_tag="P5079_10380"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRY30426.1"
                     /translation="MKKRIAEKIRKTSETAVRLKLNLDGKGRMDVDTSIPFLDHMLEL
                     FAKHGNFDLFVKAKGDTLIDDHHLVEDIGIVLGETIADALGQKRGITRYGQVLRPEKS
                     GGAGRALTPMDETLSYVALDLSGRPFLSFRAKFPPQSKAPFSFELLEDFFQALAFNAR
                     MNLHIRLLQGRNNHHIAESLFKGFGRALAQAVSLDPRRWRRIPSTKGSL"
     gene            1389..2033
                     /gene="hisH"
                     /locus_tag="P5079_10385"
     CDS             1389..2033
                     /gene="hisH"
                     /locus_tag="P5079_10385"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRY30427.1"
                     /translation="MIRKRKEPIVGVLDYGMGNLRSVAKSFEAVGAKVRVSSSRRVLA
                     GSDLLVVPGVGAFDAAMSVLRRKKLDGFIRRWVAGGKPYFGICLGLQLLFEKSDESPR
                     VRGLGILPGRVRRFSVDRALKVPHMGWNRVAWSGARPGPARPDCFYFVHSFFPQPKDR
                     SIVWGKTSYGRPFCAAVAAGNIRATQFHPEKSGSNGLKFLKRLMAGIERGRWGS"
     gene            2035..2760
                     /gene="hisA"
                     /locus_tag="P5079_10390"
     CDS             2035..2760
                     /gene="hisA"
                     /locus_tag="P5079_10390"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRY30428.1"
                     /translation="MLVVPAVDIKAGRCVRLVHGDPRRETVYSADPVETARRWASQGA
                     RRVHVVDLDAALGTGTNLDLIFRIRQAVDCEIQVGGGLRDIETVRRVLSVGIDRVVLG
                     TALLKSPDWVKKAVDEFKGSIVAGVDASPSGEVKSEGWTENLGFPIIGTILHVEKLGI
                     REVIYTDISKDGTLSGPNIETTRLILKKTKMGVYASGGVSGLDDIKALKLLEPEGLRG
                     CIVGKALYDGRLAFADALAVAGQ"
     gene            2843..3598
                     /gene="hisF"
                     /locus_tag="P5079_10395"
     CDS             2843..3598
                     /gene="hisF"
                     /locus_tag="P5079_10395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRY30429.1"
                     /translation="MLMRRIIPCLDVDAGRVVKGTRFVNLRDAGDPVEVAKRYNREGA
                     DEVVFLDITASSGKRPILLDVVRRTAEQVFIPLTVGGGLRTLEDIRTLLSAGADKVSL
                     NTAAVRNPDFVRAAARRFGAQCTVVAIDAKQTGPTRWEVYTHGGRTATGLDAVAWAKK
                     VVRLGAGEILLTSMDADGTKAGYDLQLLRRICAAVPVPVIASGGAGEKRHFLEAFQAG
                     ADAALAASLFHFKELSIAGLKRFLARRRVPVRM"
     gene            3630..>3825
                     /locus_tag="P5079_10400"
     CDS             3630..>3825
                     /locus_tag="P5079_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRY30430.1"
                     /translation="MQKPDWIKDVKYDDKGLIPAVVQDHRDGTILMVAYMNEESLLYT
                     LETRRATFFSRSRNKLWKKGE"
BASE COUNT          706 a         1200 c         1223 g          696 t
ORIGIN      
        1 ggcctacggc acccggacca tcccgaaagt ggacctgatc gtcgggcccg gcggggcctg
       61 ggtcaccgag gccaagcgcc aggtcttcgg cgaggtgggg atcgacctca tcgccggccc
      121 cagcgaggtg gcggtgttgg cggatcggac gacgtccccg cggttcgccg cggccgacct
      181 gatggcccag accgagcacg acccgctggc ccgggcggtc cttctgtccc ccgacgcccg
      241 gacgatcgcg gcggtcaaag gtctggtgga gaaacgattt cggccccagt gttcttttgt
      301 gaagacgaaa aactgggccg acgccgtggc gaaagccaac gcgttggcgc cggaacacct
      361 ctcccttctg gtgaaaaaac cggagcgcct tctgaacggc gtccgcaacg ccggggcgat
      421 tttcctcggc ccctggtcgc cggtggcggc gggggattac tgggccggac cgtcgcacgt
      481 gttacccacc ggccgcgcgg cgcggttcat gtccggcctg tccgtgcaga cgtttttgaa
      541 acgttccagc atcatccgcc tgtcggaacc gctgatgcgg aaaaaagcgc ggaccatcgc
      601 ccggctggcg gaggcggagg gactcgttta tcacgccgcc tccctgaagg cgcgcctgaa
      661 ccgctctcgc tgaaggaaga ggacctttta tgaaaaaacg catcgccgaa aaaatccgca
      721 agacctcgga aaccgccgtc cgtctgaaac tgaacctgga cggaaagggc cggatggacg
      781 tggacacctc catcccgttc ctcgaccaca tgctggagct cttcgccaaa cacggcaact
      841 tcgacctctt cgtgaaggcg aagggagaca ccctcatcga cgaccaccac ctggtggagg
      901 acatcggcat tgtgttgggc gagaccatcg ccgacgcctt gggccagaaa cggggcatca
      961 cgcgatacgg gcaagtcctg cgtcccgaaa aatccggcgg cgccggccgc gccctgacgc
     1021 ccatggacga gaccctctcc tacgtggcgc tggacctgtc cggccggccg ttcctctcgt
     1081 tccgggccaa gttcccgccc cagagcaagg cgccgttctc cttcgaactc ctggaggatt
     1141 tttttcaggc gctggctttc aacgcccgca tgaacctgca catccggctg ctgcagggcc
     1201 ggaacaacca ccacatcgcc gaatccctct tcaaggggtt cggccgcgcc ctggcccagg
     1261 cggtgtccct cgacccgcgg cgctggcgac gtatcccctc caccaagggg tccctgtaat
     1321 atccccggcc cttcggattt tcgttctccc ggccgaaggt ccgggaggga aaaaccaacg
     1381 agactcgaat gattcgaaaa agaaaagaac caatcgtggg cgtgctggac tatgggatgg
     1441 ggaatctccg ttccgtggcc aaatcgttcg aagccgtcgg ggcgaaggtg cgcgtgtctt
     1501 cgtcgcgccg cgtcctggcg ggatccgacc ttttggtggt gcccggcgtg ggcgccttcg
     1561 acgccgccat gtcggtcctg cgccggaaga agctggacgg tttcatccgc cgctgggtgg
     1621 ccggcggaaa accgtacttc ggaatctgtc tgggcctgca gctgctcttt gagaaaagcg
     1681 acgagtcgcc ccgcgtgagg ggcctgggca tcctgccggg gagagtgcgc cgtttttccg
     1741 tcgatcgcgc cctcaaggtc ccgcacatgg gctggaaccg ggtggcctgg tccggggccc
     1801 ggcccggccc ggcccggccg gattgttttt atttcgtaca ctcgtttttc ccgcagccga
     1861 aggaccgctc catcgtgtgg ggaaaaacgt cctacggccg gccgttctgc gcggcggtgg
     1921 cggccggcaa catccgcgcc acgcagtttc acccggaaaa aagcggatcg aacggattga
     1981 agttcttgaa acggctgatg gccggcatcg aacgcggccg gtgggggagc tgatatgctc
     2041 gtcgtcccgg cggtggacat caaggcgggg cgctgcgtgc gcctggtcca cggcgatccc
     2101 cgccgggaaa cggtgtattc cgccgacccg gtcgaaacgg cccgtcggtg ggcctctcaa
     2161 ggcgcccgcc gggtccacgt ggtggatctg gacgccgcgc tcgggacggg gaccaacctc
     2221 gatttgatct ttcgcatccg ccaggcggtg gattgcgaga tccaggtggg cggcggcctg
     2281 cgcgatatcg aaacggtacg acgggtgctt tcggtcggca tcgaccgggt cgtcctcggc
     2341 accgcgctcc tgaaatcgcc cgactgggta aagaaagcgg tggacgagtt caagggctcc
     2401 atcgtggcgg gcgtggacgc ttctcctagc ggagaagtaa aatcagaagg ttggacggaa
     2461 aaccttggtt ttccgataat cgggacaatt ttacatgtcg aaaaacttgg catccgtgaa
     2521 gttatctata ccgacatttc gaaggatggt actctttcgg ggccaaatat agaaaccacg
     2581 cgattgattt taaaaaaaac caaaatgggc gtttatgcct ccggaggggt ctccgggttg
     2641 gacgatatca aggcgctcaa gttactggaa cccgaggggc tccgtggctg catcgtcggc
     2701 aaggccttgt atgacggccg gttggctttt gcggacgccc tcgccgtcgc gggccaatga
     2761 atacggtcat gtttcaggcc cattgtctgc cttttttgtt gcgtcatagc accacttcgc
     2821 cattgcacta tttcaccatc ttatgctgat gcggcgcatc atcccctgtt tggacgtgga
     2881 cgccgggcgc gtggtgaaag gcacccggtt cgtcaacctg cgcgacgcgg gagacccggt
     2941 ggaggtggcc aaacgctaca accgggaagg cgcggacgag gtcgtgttcc tcgacatcac
     3001 cgcgtcctcc ggcaaaaggc ccatcctctt ggacgtggtg cggcgcaccg cggaacaggt
     3061 cttcatcccg ctgacggtgg gcggcggtct tcgcacgttg gaggacatcc gcaccctgtt
     3121 gagcgccggt gcggacaaag tctctttaaa caccgccgcg gtgcggaatc ccgactttgt
     3181 gcgggccgcc gcccgacggt tcggcgccca gtgcaccgtg gtggccatcg acgccaaaca
     3241 gaccggtccg acccgatggg aagtttacac ccacggcggc cggaccgcca ccgggctcga
     3301 cgccgtggct tgggcgaaaa aagtcgtccg tctcggcgcc ggcgagatat tgctcaccag
     3361 catggacgcc gacgggacca aggccgggta cgacctgcaa ctcctccggc gcatctgcgc
     3421 cgcggtcccc gtacccgtca tcgcctccgg cggggcgggc gagaaacgac attttttgga
     3481 agcgtttcag gcgggcgcgg acgccgccct ggccgcctcc ctgttccatt tcaaggaact
     3541 ctccatcgcc ggattgaagc gtttcctggc ccggcggaga gtccccgtcc gcatgtagcc
     3601 gttgaattaa tttcaaggag atcgaaacca tgcaaaaacc ggactggatc aaagatgtaa
     3661 aatacgacga taaagggctc atcccggcgg tggtccaaga ccaccgggac gggaccatcc
     3721 tgatggtggc ctacatgaac gaggagtcgc tgctctacac gctggagacg cggcgggcga
     3781 cctttttcag ccggtcacgc aacaaactct ggaagaaggg cgagg
//
LOCUS       DAOTFD010000237         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17773_length_3818_cov_4.156790, whole genome shotgun sequence.
ACCESSION   DAOTFD010000237 DAOTFD010000000
VERSION     DAOTFD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17773_length_3818_cov_4.156790"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5079_10405"
     CDS             complement(<1..603)
                     /locus_tag="P5079_10405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30431.1"
                     /translation="MNGILRKIAVGGRREDTLLVGALLTFFVLAALYRFHSPGTIYEN
                     LWDEWESVRLLENFRSTRAIPIPSYPIFHGQIMKYFLAPAFLVGGANFTTLRLAPVLF
                     GVAVLLMFWYSLRRFGGRGVALLALFLTVIHPSFVLGMKIGSYQTSSMLIFSMTSVAC
                     LQFFWDAKKARYLFAAALAAGLGLGARLWFVWFLFALAAAT"
     gene            838..2025
                     /locus_tag="P5079_10410"
     CDS             838..2025
                     /locus_tag="P5079_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRY30432.1"
                     /translation="MKFSDILKKKDAGTPSALDVPPPAPTPPPKSPAPAAPAAPSAPP
                     TPAPSIYKSTPPPAARPPEESDEVIEQRVTAHALKAMEIYSEAVSVARTVYTAAAKNE
                     PIPSLTPVKEALFKIVAVLESGNQELLAMADRATPDNYLFAHVVNVAIFAIRLGQSRG
                     LSHPDLQALSLSAFLHDLGMLPYLSLAQKPALLNQEELAQIQQHPLETRKLLSLFSDL
                     SGPLKDAVHDSAGQVHERIQGNGYPERRAGENIHPFARIIGVCDVYEAVTHPRPQRPR
                     ALPHQALRVMIEEHERSFEPGIVKTLIESLSLYPTGSFVRLSSGEIGRVIFTDPGLPT
                     RPKVKMLLDARGNRLPGPKIVNLALQPILYVADAVDETQIQTSDKRLLLELRAQRWWV
                     KGL"
     gene            2184..2897
                     /locus_tag="P5079_10415"
     CDS             2184..2897
                     /locus_tag="P5079_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30433.1"
                     /translation="MTKAHPDFWQIALEPAALEQPREVPVVEEKTGRFSVSSGRFHGE
                     FSRKERRGRLRVRNTAAVTTFLRAFLSHVLTKERGFLLHGAGVRVNDGACLLAGVSGS
                     GKSTLSRMAAPGDVLSDELVLAHRGDARWQAYGTPFYGDVRRNQPAGYPLRAVFLLEK
                     AGEWSAERLSQSDAARGLLKCVLNFSAGREGYSSLLDNTADLTAAVPCLRLRFAKKRP
                     HGKSLTATLDGLLRSEGLS"
     gene            2894..3172
                     /locus_tag="P5079_10420"
     CDS             2894..3172
                     /locus_tag="P5079_10420"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRY30434.1"
                     /translation="MSPRSVKYRQASHLAQRRIAGELFVVDPRHRCLHQPQGAGDPLW
                     EWLKKGATVEELVQNLLRDYDVPRDTAEKDVRRFLRELKRKKIVEEVS"
     gene            3169..>3818
                     /locus_tag="P5079_10425"
     CDS             3169..>3818
                     /locus_tag="P5079_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30435.1"
                     /translation="MRVRGRESLSWRMMEAAEKAGAPHSAMVELTWRCPLRCRHCYLP
                     AAERARREEPAELGTGELRRVFDEMAELGVLYLTLSGGEICRRPDLPELVAHARRNSF
                     DVRLFTSGALVTPPLAAELARRGVARAEISLYGREETHDRITGVPGSYGRSLAGARLL
                     KANGIAVTIKAPLMNLNIPDYPFLVRLAEEEGFLYGFDPTVVPRDDGDKTPTAFRCS"
BASE COUNT          780 a         1266 c         1145 g          627 t
ORIGIN      
        1 agtggcggcc gccaaagcga aaaggaacca cacgaaccaa agccgcgcgc ccaaccccag
       61 gccggcggca agggcggccg caaaaagata ccgcgctttt ttcgcgtccc aaaaaaattg
      121 cagacacgcc acagaagtca tcgagaaaat caacatggag gacgtttgat aggaaccgat
      181 cttcataccc agcacaaaag aggggtggat caccgtcaaa aaaagcgcca gcaacgccac
      241 gccgcggccc ccgaaacgtc tcagggaata ccagaacatc agaagcacgg ccactccgaa
      301 caaaacgggg gccaagcgca acgtggtgaa atttgccccg cccaccaaaa acgccggggc
      361 caggaaatac ttcattatct ggccgtggaa aatcggatac gagggaatgg ggatggcgcg
      421 ggtggagcgg aaattctcca acaaccggac cgactcccat tcatcccaaa ggttctcgta
      481 gatggtgccg ggcgaatgga aacgatacag cgccgcgagg acgaaaaaag tcaaaagagc
      541 ccccaccagc aacgtgtcct cccgccggcc gccgaccgcg attttcctca atatcccatt
      601 catggggaga aacgtatcaa atacgggccg cggccacaaa ttccgcgccg ccgggaggct
      661 ccttccgtcg gcaaagagcc cgccggccca agagttcgtc cttgaaacgt ccggcgggac
      721 tttgtagact aaaacgatta taggttcctt tgttgagcgg gacccggccg gcggcgcgcc
      781 acgaccgttt tgggccgttc gtccgaaaca atgccatccc cggaggggat cagcgaaatg
      841 aaattttccg acatcctgaa gaaaaaagac gccggcacgc cgtcggccct cgacgttccg
      901 ccgccggcgc ccacccctcc gccgaagtcc ccggcccccg cggcgccggc cgcgccgtcg
      961 gcaccgccaa cccccgcgcc cagcatctac aaatccacgc cgccgccggc cgcccggccg
     1021 ccggaagaat cggacgaggt catcgaacaa cgcgtcacgg cccacgcctt aaaagcgatg
     1081 gagatctatt ccgaagcggt ctccgtcgca cggaccgtct acaccgccgc ggcgaaaaac
     1141 gagccgatcc cctcgctgac ccccgtgaaa gaggccctct tcaaaatcgt ggcggtgttg
     1201 gaatcgggca accaggaact cctggccatg gccgaccgcg ccacgcccga caactatctc
     1261 ttcgcccacg tggtgaacgt ggccattttc gccatccggc tggggcaatc gaggggtctg
     1321 tcccacccgg accttcaagc gctgtccctg agcgcgttcc tgcacgacct cggcatgttg
     1381 ccttacctgt ctctggccca aaaaccggct ctcttgaacc aggaagaact ggcgcagatc
     1441 caacagcacc ctctggaaac gcggaaactc ctgtcgcttt tctccgacct ttccggccct
     1501 ctcaaagacg ccgtgcacga ttccgccgga caagtgcacg aacgtatcca ggggaacggc
     1561 tatccggaac ggcgggcggg ggaaaacatc catcctttcg cccggatcat cggggtgtgc
     1621 gacgtgtatg aggcggtgac gcacccgcgt ccccagcggc cgcgcgccct tccgcaccag
     1681 gccttgcgcg tgatgatcga ggaacacgaa cggtcgttcg aaccggggat cgtcaaaacg
     1741 ctgatcgaat ccctctccct ttatccgacc ggctcctttg tgcgtctgag ttcgggggaa
     1801 atcggccgcg tcattttcac cgacccgggc ctgccgacac ggcccaaagt caagatgctg
     1861 ctggacgccc gcggaaaccg gctgcccggc cccaagatcg tcaacctggc cctgcagccc
     1921 atcctctacg tggcggatgc cgtggacgag acccagatcc aaaccagcga taaacgcctg
     1981 ttgctggaac tgcgggccca acgatggtgg gtcaaagggc tttagcgcct ccccaccctc
     2041 cgacattcat cccccctggt ttcatgtccg gaataccctt tcctgaaaac aatccggcct
     2101 ttcgcatcgg cccggtgggg tttcatttcc atccgagttc gatcctccat aaaaaacaat
     2161 tattctcgga atatcgccgg tttttgacga aagcgcaccc ggatttttgg cagatcgccc
     2221 tggaaccggc ggcgctggaa cagccgaggg aagtgccggt ggtggaggaa aaaacagggc
     2281 gcttctccgt ctcctcggga cgattccacg gagaattctc ccgaaaagag cgccgaggcc
     2341 gattacgggt ccgcaacacc gccgcggtga cgaccttcct ccgcgccttc ttgagccacg
     2401 tcttgacgaa ggagcgggga ttccttctcc acggcgcggg cgtgcgggtg aacgacgggg
     2461 cgtgccttct ggccggcgtc tccggcagcg ggaaatccac cctgtcgcgg atggccgcgc
     2521 cgggagatgt gctctccgac gaactggtgc tggctcaccg aggcgacgcg cggtggcaag
     2581 cctacggcac gccgttctac ggagacgtac gccgcaacca accggccggt tatccgttgc
     2641 gcgccgtttt ccttctcgaa aaagccgggg aatggagcgc ggaacgcctc tcccaaagcg
     2701 acgccgcgcg agggctgttg aagtgcgttt tgaacttcag cgccgggcgc gaagggtatt
     2761 cctccctttt ggacaacacc gccgacctga cggccgccgt gccctgtttg cggctccggt
     2821 tcgctaaaaa acggccgcac ggaaagtccc tcacggcgac gctcgacggt cttcttcgtt
     2881 cggagggcct ctcgtgagcc cccgatcggt caaataccgg caggcctcgc acctggccca
     2941 acggcgcatc gcgggggaac ttttcgtggt ggacccgcgg caccgctgcc tgcatcagcc
     3001 gcagggggcg ggggacccgc tctgggaatg gctcaaaaaa ggcgccaccg tcgaggaact
     3061 ggtgcagaac ctcctgcggg actacgacgt cccccgcgac acggcggaaa aagacgtgcg
     3121 ccgctttctg cgcgagttga aacgaaagaa gatcgtcgaa gaggtttcat gagggtccgc
     3181 gggcgggaaa gtctttcgtg gcggatgatg gaggcggcgg aaaaggccgg agcgccgcac
     3241 tccgccatgg tggaactcac ctggcgctgt cctctccgtt gccggcactg ctaccttccg
     3301 gcggccgagc gggcgcggcg cgaagaaccc gccgaattgg gcaccgggga actccgccgc
     3361 gtttttgacg agatggcgga gttgggcgtg ctgtatctga cgctttccgg cggagaaatc
     3421 tgccggcggc cggaccttcc cgaactggtg gcgcacgccc gccgaaattc tttcgacgtc
     3481 cgtctcttca cctcgggcgc gctcgtcacc ccgcccctgg cggcggaact ggcgcggcgg
     3541 ggcgtggccc gggcggaaat cagcctgtac ggccgggaag aaacccatga ccgcatcacc
     3601 ggcgttccgg gttcctacgg ccgttcattg gcgggggcgc ggctcctcaa ggccaacggc
     3661 atcgccgtga ccatcaaagc gccgctgatg aacctgaaca tccccgacta cccgttcttg
     3721 gtgcggctgg cggaagagga ggggttcctc tacgggttcg acccgacggt ggtcccgcgg
     3781 gacgacggcg acaaaacgcc gaccgcgttc cgttgttc
//
LOCUS       DAOTFD010000238         3813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17803_length_3813_cov_4.200373, whole genome shotgun sequence.
ACCESSION   DAOTFD010000238 DAOTFD010000000
VERSION     DAOTFD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17803_length_3813_cov_4.200373"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..>1261)
                     /locus_tag="P5079_10430"
     CDS             complement(233..>1261)
                     /locus_tag="P5079_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY30436.1"
                     /translation="KASDLHLTVGSPPVLRIDGLVHPTNLEKLTPEMCQRLVYSLLND
                     VQKQRFEKENELDLSFGIKGLGRIRMNVFRQRGSVGAALRSIPSRFMSFDELGLPPII
                     NDIIKSPRGLVLVTGPTGMGKSTTLASMIDFINESRASHIITIEDPIEYLHFHKRSVV
                     NQREVGQDTNSFHGALKYILRQDPDVILVGEMRDHETIAAALTIAETGHLVFSTLHTN
                     DAPSTVNRILDSFPPHQQEQARSQLSMTLQAIISQNLLMHASGAGRVLASEVLVVTPA
                     VRNLIREQKVEQIALAMQTGGKLGMQTMNQSLYDLYVRRQITYQTAVEHSLDQEDLKR
                     MLPKATGL"
     assembly_gap    1262..1271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1351..3171)
                     /locus_tag="P5079_10435"
     CDS             complement(1351..3171)
                     /locus_tag="P5079_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY30437.1"
                     /translation="MKKKLGDILVDVGIITREQLDQAIEIQRAKGGKLGDILKEMNFC
                     SEDVLLAFLGRQCGVSYISLAEYGDIPQDVINMVPESVARHQTLIPIARDGNVLTIAM
                     SDPLNVFATDDLKLMTGCDIKVVIASEKEIKQSIDRHYLQTSSVGVATSNVEEGSVQN
                     YKPGEMPASMKDVVQKIEKDTPVEIETVTPVAGADVFTVNVEADEAPVIKMVNVLLAN
                     AAKAGASDIHIEPYENVFRIRYRIDGVLHNQPAPPRKYQNAIISRVKVMAQMDVAERR
                     LPQDGRIKIKIEGKEIDLRVSTAPTAHGEKVVMRLLDSSGIKVDLTDLGFDPEPLILF
                     KKIISSPHGMILVTGPTGSGKSTTLYATLSALNRSDINILTIEDPIEYKIEGINQVQA
                     RADIGLTFAVGLRNFLRQDPDIILVGEIRDKETAEIAINAALTGHLVLSTLHTNDAPG
                     AITRLDNMGVESFLTVSTLLAVVGQRLIRVICDHCKETSSQSVEHLSSVGINLPHTKI
                     SSRGKSVTLSRGKGCDHCAGTGYHGRMAIYELMPVTDPIRELILARASGHQIKQVARR
                     QGMLTLREAAVQKMMTGITTLEEVLRVTQSDADAETESKR"
     gene            complement(3230..>3813)
                     /locus_tag="P5079_10440"
     CDS             complement(3230..>3813)
                     /locus_tag="P5079_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRY30438.1"
                     /translation="GAWGEAAGDAAATAVNGAAPEAGAPPAAEAAPATDAAVSPDPSA
                     RALAAIGIESARLYERFAGETPRVAFLSFSTKGSAEDKSVLKMREAAALVRKKAPALA
                     ADGELQADAALSPDIAAQKGAGDSPAAGMANVLVFPNLDAGNIAYKLVQHLGGARAVG
                     PYLAGLSKPLSDLSRGCTDEDIVDAAALLVLAA"
BASE COUNT          576 a         1068 c         1372 g          787 t
ORIGIN      
        1 catttcgctt tttggagggc ggcccggccg ttgaaggcga tgagcacctc gtaaaaatcg
       61 ccgagccagt cctcgatgag ggaggcgagg ttctcctcgt cctcggccac gaggatcttc
      121 tttcgttgga cgacgccgat gtcgatggtg tggtcgggag gggcgttcat gggtggggcc
      181 gcgggcgagg ggtcatggga tgtcggcggg cctcagacga acgagccggc cttcacaggc
      241 cggtggcctt ggggagcatc cgtttcaggt cttcctggtc gagcgaatgc tcgacggcgg
      301 tctgataggt gatctgccgg cgcacgtaga ggtcgtagag ggactggttc atggtctgca
      361 tgccgagttt cccgccggtt tgcatggcca gggcgatctg ctcgaccttc tgttcccgaa
      421 tgaggttgcg caccgccggg gtgaccacga ggacctcgct ggccaacacg cgccccgcgc
      481 ccgaggcgtg catgaggagg ttctggctga tgatggcctg cagggtcatg gagagctggg
      541 aacgggcctg ttcctgctgg tggggcggaa aggagtcgag gatgcggttc accgtcgagg
      601 gggcgtcgtt ggtgtgcagc gtggagaaca ccaagtggcc ggtctcggcg atggtgaggg
      661 cggcggcgat ggtttcgtgg tcgcgcattt ccccgaccag gatgacgtcg gggtcctggc
      721 gcaggatata tttgagggcg ccgtggaaac tgttggtgtc ttggcccacc tcgcgctggt
      781 tgacgacgga acgtttgtgg aaatgcaggt attcgatggg gtcttcgatg gtgatgatgt
      841 gggaggcgcg gctctcgttg atgaaatcga tcatgctggc cagggtggtg gatttgccca
      901 tgccggtggg gccggtgacg agcaccagtc cgcgcgggga cttgatgatg tcgttgatga
      961 tggggggcag ccccagttca tcgaaggaca tgaagcggga cgggatggac cgcagcgccg
     1021 ccccgacgct gccgcgctgc cggaacacgt tcatgcggat gcggcccagc cctttgatgc
     1081 cgaacgagag gtcgagttcg ttctctttct cgaaacgctg tttttggacg tcgttcagca
     1141 gggaatagac gaggcgctgg cacatctccg gcgtgagttt ttccaggttg gtggggtgca
     1201 cgaggccgtc gatgcggagc acgggagggg agccgacggt gaggtggagg tcggaggcct
     1261 tnnnnnnnnn ncaggatgag caggtcgttc atgtttacca tgattttttc ccctctctga
     1321 ggaaaacggg tggatttttt tttcggagcg tcatcgtttg gattccgttt cggcgtcggc
     1381 gtcggactgg gtgacgcgca ggacttcctc cagcgtggtg atgcccgtca tcattttttg
     1441 gacggcggcc tcgcgcaggg tcagcatgcc ctgccggcgg gccacctgct tgatctgatg
     1501 gcccgaggcc cgcgccagga tcagttcccg gatggggtcg gtcacgggca tgagttcgta
     1561 gatggccatc cgcccgtggt aaccggtgcc ggcgcagtgg tcgcatccct tgccccggga
     1621 gagcgtcacc gatttgccgc gcgaggagat cttcgtgtga ggcaggttga tgccgaccga
     1681 ggacaggtgc tcgacggact gggaagaggt ctctttgcag tggtcgcaga tgacgcggat
     1741 cagtcgctgg cccaccacgg cgaggagggt ggagacggtc agaaaggatt cgacccccat
     1801 gttgtccagg cgggtgatgg cgccgggggc gtcgttggtg tgcagcgtgg agaggaccag
     1861 gtgtcccgtg agggcggcgt tgatggcgat ctcggcggtc tccttgtccc ggatttcgcc
     1921 gacgaggatg atgtccgggt cctgccgaag gaagttcctc aagccgacgg cgaaggtcaa
     1981 tccgatgtcc gcccgggcct gcacctggtt gatgccctcg atcttgtatt cgatggggtc
     2041 ttcgatggtg aggatgttga tatcggaacg gttcagcgcc gagagggtgg cgtagagggt
     2101 ggtggatttg cccgaccccg tcggcccggt gacgaggatc atgccgtgcg gcgaggagat
     2161 gatcttcttg aagaggatga gcggttccgg gtcgaagccc aggtccgtca ggtccacctt
     2221 gatgccggag gaatcgagga gccgcatgac cactttttcg ccgtgggcgg tgggggcggt
     2281 ggagacgcgc aggtcgatct ccttgccctc gattttgatc ttgatgcgtc cgtcctgggg
     2341 caggcgcctc tcggccacgt ccatctgggc catgaccttg acgcgggaga tgatggcgtt
     2401 ctgatatttg cgcggcggcg cgggctggtt gtgcagcacg ccgtcgatgc ggtagcggat
     2461 gcggaaaacg ttttcgtagg gttcgatgtg gatgtcggag gcgccggctt tggcggcgtt
     2521 ggccaggagc acgttgacca tcttgatgac gggcgcctcg tcggcctcga cgttgacggt
     2581 gaagacgtcc gccccggcga ccggcgtgac ggtctcgatc tccaccgggg tgtccttctc
     2641 gattttttgg accacgtcct tcatggaggc cggcatctcg ccgggcttgt aattctgtac
     2701 ggagccctcc tcgacgttgc tggtggccac gcccaccgag gaggtctgca ggtaatggcg
     2761 gtcgatggac tgcttgatct ctttttccga ggcgatgacg accttgatgt cgcagccggt
     2821 catcagtttc aggtcgtcgg tggcgaagac gttcagcggg tccgacatgg cgatggtcag
     2881 cacgttcccg tcgcgggcga tggggatgag ggtctggtgg cgcgccaccg attccgggac
     2941 catgttgatg acgtcctgcg ggatgtcgcc gtattcggcg agcgagatgt aggagacgcc
     3001 gcactggcgg cccaggaagg cgaggagcac gtcctcgctg cagaaattca tctccttgag
     3061 gatgtccccc agtttgccgc ccttggcgcg ctggatctcg atggcctggt ccaattgttc
     3121 ccgggtgatg atgccgacat ccacgaggat gtccccgagt ttctttttca tggagccggt
     3181 gttgcgaacg accatgcgcg ccctttctcc ttcggatttt aaaaaagttt caggccgcca
     3241 ggaccaacaa agcggcggcg tccacgatgt cctcgtcggt acagccccgc gaaaggtccg
     3301 acagcggctt ggagagtccg gccagatacg gtcccaccgc ccgggcgccc cccaggtgct
     3361 ggacgagttt ataggcgatg ttgccggcgt ccaggttcgg gaagaccagc acgttcgcca
     3421 ttcccgccgc cggggaatcc ccggcgcctt tctgggcggc gatgtcgggg gagagcgcgg
     3481 cgtccgcctg aagttccccg tcggcggcca gcgccggcgc tttcttgcgc accagcgccg
     3541 cggcctcccg catcttcaaa acgcttttgt cctcggcgct gcccttcgtc gaaaaggaca
     3601 ggaacgccac ccggggcgtt tcgccggcga accgttcgta gagccgcgcc gattcgatcc
     3661 cgatggccgc cagggcccgc gctgacgggt cgggcgagac cgcggcgtcg gtcgcgggcg
     3721 ccgcctcggc cgcgggtggc gcccctgcct ccggagcggc cccgttcacc gcggtcgccg
     3781 cagcgtcccc ggcggcctcg ccccaggcac cgt
//
LOCUS       DAOTFD010000239         3808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17834_length_3808_cov_3.604317, whole genome shotgun sequence.
ACCESSION   DAOTFD010000239 DAOTFD010000000
VERSION     DAOTFD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17834_length_3808_cov_3.604317"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="P5079_10445"
     CDS             <1..1414
                     /locus_tag="P5079_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30439.1"
                     /translation="LRAAPPVLTLALALVVLAVFSSITVERNGVWRDDATFWRTASLQ
                     SPQAARTWYNLGVLAQKRNDLEHAERYYRKALALRPDYVEPRTNLLAIVDIEKQGRPD
                     GYESILAKQPRTYVPYLNAANALMKEKKYAEAAALYKKIQHGFPDTSYPWVDVFLNLA
                     KAYALMGEPALSEATFDRCVGDRGKSARARRDLGKFYDDAGHYAEAEKHYQKALVFRP
                     DWAAAEVDLGVVYHHQGRFPEALRMIQAGLRHAPDRAVAHYNLGIVYSDMSESALAEK
                     SFREAIRLSKDYTDAHYNLAVVHQRQGKMTEAIAGYEKVTTLDPRRVEALSNLGGCYE
                     QLGDLQKAEKYCRRAVQVDPTRSCPLVTLGNIYQKRGLFAEATGFYQNAIRIDPIDVM
                     AYNNLGACYLRLGEAKKAEQEFASAIRLNPAFADAYYNLGLALERQGQRDRAVAAWKS
                     ALAADPTHTGARQALERIAG"
     gene            1411..2520
                     /locus_tag="P5079_10450"
     CDS             1411..2520
                     /locus_tag="P5079_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY30440.1"
                     /translation="MRFFKMEGVTMKENENSKTTLKNRAGGLAAGFGLLIAGLMVFTG
                     PEASAGKASSNNYRLTRTSINQGGQSKASATTYKMKGSVGETSYGVMSGTTRNVADGL
                     MKIMFYPRTISDIRPSQGADEGSIKLEWTAPGADGGVVTASKYIVKYSTYPDAITDQS
                     YFETTATLFKVAVPNAPNTQETITLTGLTPGTSYYVAIEARDADENQGDLSKSTYTWA
                     QVTVLSVDISSDTYGFGTFTASTSTVSTSTIRVVNNGTVLSTWNLKASTITTGSPWQI
                     TDGSLSPGDLFRLSAAFHLVQPSTTAFGTEDRMTDTDAASDASSKFTINGSSTGYSVP
                     AGEYRNFWLMLETPLSSSTSDEQQIRVTVTATPTP"
     gene            2534..3433
                     /locus_tag="P5079_10455"
     CDS             2534..3433
                     /locus_tag="P5079_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30441.1"
                     /translation="MARIRQFVSFFLSFGAAFFAVAVGQAGLGLSTTFVDVVMDELKT
                     GQGYVSGQRDFRPFTVTNHSSKPVKVVIEPEISPQGVLEGYEPIPSIAWLKVTPQRLS
                     LKPGETKTAHLRLDIPKGEQWKQRHFQVNLRTRTDEGTVAVGLESRLRFSVEAVRPES
                     ATPRTAALSKLEQVRFEPQELYFNKVPAGKEMRLTDDRRSILKIANKGNTPVRLSLRP
                     GYFQSAGLPEGYVSTPNPDFLEVAPRSVEVPADSAVTVNLQVRIPPGTVHYGKKYAFL
                     LRASVDDGITEPVLARVYVRVRG"
     gene            3435..>3808
                     /locus_tag="P5079_10460"
     CDS             3435..>3808
                     /locus_tag="P5079_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30442.1"
                     /translation="MNTNTSVSIVQPGLAETLLRGAGEAALLTLSAAVPLAVGFSLND
                     SFDLPKMTIVAFGAIFVAAVAAVRALSAGKLIWRSTPLDVPLVGLLAVSLVAALLSIH
                     RPISFFGQYREYAFGWLPLLAV"
BASE COUNT          742 a         1193 c         1157 g          716 t
ORIGIN      
        1 ccttcgagcg gcccctcccg tcctgaccct tgccctcgcc ctcgtcgtcc tggcggtgtt
       61 ttcctcgata acggtcgagc ggaacggcgt ttggcgcgac gatgccacgt tctggcggac
      121 ggccagcctt caatcgcccc aggcggcccg gacgtggtac aacctggggg ttctggctca
      181 aaagcggaac gacctcgaac acgccgaacg gtattaccgc aaggccctgg cgctgcggcc
      241 ggactacgtg gagccgcgca ccaacctcct ggccatcgtc gacatcgaaa aacaaggccg
      301 gcccgacggt tacgaatcca ttctggcgaa acagccgcgc acctacgtgc cctacctcaa
      361 cgccgccaac gctctcatga aggagaaaaa atacgccgag gcggcggcct tgtacaaaaa
      421 gatacagcac gggtttccgg atacgtcgta tccctgggtg gacgttttcc tcaacctggc
      481 caaggcctat gccctgatgg gcgagccggc gttgtccgag gccacgttcg accgctgcgt
      541 gggggacagg ggaaaatcgg cgcgggcgcg gcgggacctg ggaaaattct acgacgacgc
      601 cggacactac gctgaggcgg agaaacatta tcaaaaggcg cttgttttcc ggccggattg
      661 ggccgctgcc gaggtggacc tgggtgtggt ctatcaccac cagggccggt tccccgaagc
      721 ccttcggatg attcaggccg gtctgcgcca cgcgcccgac cgcgccgtcg cccattacaa
      781 cctcggcatc gtttacagcg acatgagcga gtcggccttg gccgagaaat cgttccggga
      841 agccatccgg ctcagcaagg attacaccga cgcccattac aacctggcgg tggtccatca
      901 gcggcagggg aaaatgacgg aggccatcgc ggggtatgaa aaagtgacga cgctggatcc
      961 gcgccgcgtc gaagcgttga gcaatctggg ggggtgttac gaacaactcg gagaccttca
     1021 gaaagccgaa aaatattgcc gccgggccgt gcaggtagac ccgacccgct cctgtccgct
     1081 ggtgactttg gggaacattt atcagaaacg cggacttttc gccgaggcga ccggttttta
     1141 ccagaacgcc atccgcatcg atcccatcga cgtgatggcc tacaacaatt tgggcgcgtg
     1201 ttacctgcgt ttgggggagg cgaaaaaggc cgaacaggag ttcgcgtccg ccatccgcct
     1261 caacccggcg ttcgcggacg cgtattacaa cctcgggctc gctttggagc ggcagggaca
     1321 gcgggatcgg gcggtggcgg cttggaagtc ggcgttggcg gcggacccga cccacacggg
     1381 cgcccggcaa gcgctcgagc ggattgcggg atgaggttct tcaaaatgga aggcgtgacc
     1441 atgaaggaaa atgagaactc caaaacgacg ttgaaaaatc gtgcgggcgg tctggccgcc
     1501 gggttcggcc tgctcatcgc cggcctcatg gtcttcaccg gccccgaggc gtcggcgggc
     1561 aaggcctctt cgaacaatta ccggctcacc cgcacctcca tcaaccaggg cggtcagtcg
     1621 aaggcctcgg ccaccaccta taagatgaaa gggagcgtcg gcgaaacctc ttacggtgtg
     1681 atgagcggca ccacccggaa cgtggccgac ggtctgatga agatcatgtt ctacccgcgc
     1741 accatcagtg acatccggcc ttcccaggga gccgacgagg gctccatcaa gctcgaatgg
     1801 accgcgccgg gcgccgacgg gggagtggtc accgcctcga aatacatcgt gaaatattcc
     1861 acctatccgg acgccatcac cgaccaatcc tattttgaga ccaccgccac cctgttcaag
     1921 gttgccgtgc ccaacgcgcc aaacacgcag gagaccatca ccctcaccgg cctgacgccc
     1981 ggcaccagct attacgtggc catcgaggcc cgggacgcgg acgaaaacca gggcgacctt
     2041 tccaagtcca cctacacctg ggcacaggtc accgtcttgt cggtggacat cagttcggac
     2101 acctacgggt tcggcacctt caccgcctcc acctccacgg tttcgacgtc gaccattcga
     2161 gtggtcaaca acggaactgt tctttccacc tggaacctga aggcctccac gatcacgacg
     2221 ggaagcccct ggcagatcac cgacggcagt ttgtcgccgg gcgacctgtt ccggctcagt
     2281 gcggctttcc atctggtgca accgagcacc accgctttcg gcacggaaga ccgcatgacc
     2341 gacaccgacg ccgcttcgga tgcgtcctcc aaattcacca tcaacggatc ttccaccggc
     2401 tattcggtgc cggcggggga ataccgcaat ttctggttga tgctggaaac ccccttgtct
     2461 tcaagtacct cggatgagca gcaaattcgc gtgacggtga cggcgacccc gactccgtaa
     2521 aaagaggatg gccatggccc ggatacggca gttcgtttcc ttcttccttt ccttcggcgc
     2581 ggcgttcttt gccgtggccg tgggacaggc cggtctcggc ctcagcacca cctttgtgga
     2641 cgtggtcatg gatgagctga agacgggcca gggttatgtt tccggtcaac gggatttccg
     2701 accctttacc gtcaccaacc acagttccaa accggtgaaa gtcgtgattg agccggaaat
     2761 ctcgccgcag ggggtgctgg agggctatga gccgattccg tccattgctt ggctgaaggt
     2821 gacgcctcaa cgcctgtcct taaagcccgg agagacaaag accgctcacc ttcggctgga
     2881 cattccgaaa ggcgaacaat ggaaacagcg gcatttccag gtgaacctgc gcacgcggac
     2941 cgacgagggg acggtggccg tcggcttgga gagccgcctg cgtttttcag tggaagccgt
     3001 ccggccggaa tccgcgacgc ctcgaacagc cgccctctcg aaactggaac aggtgaggtt
     3061 cgagccccaa gaactctatt tcaacaaggt gccggcgggt aaagagatgc gcttgacgga
     3121 cgatcgccgc tctattttga aaatcgcgaa caaggggaac acgcccgtcc ggctcagcct
     3181 gcggccgggg tattttcagt ccgccggttt gccggaggga tatgtgtcca cccccaaccc
     3241 ggatttcctg gaggtggccc cccgttcggt ggaggttccg gcggattccg cggtgacggt
     3301 gaacctgcag gtgcgcattc cgccgggaac ggtgcactac ggcaagaaat acgctttttt
     3361 gttgcgcgct tccgtggacg acggcatcac ggaacccgtt ctggcacgag tctatgttcg
     3421 ggtgagaggt tgacatgaac acgaacacgt cggtgtccat cgtgcaaccg ggccttgccg
     3481 agaccctcct gcggggggcg ggggaggcgg cgttgctgac cctttcggcg gcggtgccgc
     3541 tcgccgtggg gttctccctc aacgattctt tcgatctgcc gaagatgacg atcgtcgcct
     3601 tcggcgcgat ttttgtcgcc gccgtggcgg ccgtgagagc tctgagcgcc gggaaactca
     3661 tctggcgttc cacccctttg gatgttccac tcgtcggcct tttggcggtt tccctcgtcg
     3721 cggcgttgct ctccatccac aggccgatct cttttttcgg ccaataccgc gagtacgcgt
     3781 tcggatggct tcccctgctg gcggtttg
//
LOCUS       DAOTFD010000240         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17841_length_3807_cov_3.248134, whole genome shotgun sequence.
ACCESSION   DAOTFD010000240 DAOTFD010000000
VERSION     DAOTFD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17841_length_3807_cov_3.248134"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="P5079_10465"
     CDS             <1..528
                     /locus_tag="P5079_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRY30443.1"
                     /translation="GKFVFVQLGAPSRTHIPRYQNFLTDVEAMAERINEKFKGRDYKP
                     IVFIEKHHSHREIAPFFKGADVCLVTSLHDGMNLVAKEFVSARDDNGGVLILSQFTGA
                     ARELRDALVVNPYDIEKTADALREALEMPAEEKTNRMKKMRETVKEHNIYRWAGTLIE
                     ELTRVRIGPAGGAGT"
     gene            532..1323
                     /gene="otsB"
                     /locus_tag="P5079_10470"
     CDS             532..1323
                     /gene="otsB"
                     /locus_tag="P5079_10470"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRY30444.1"
                     /translation="MKNLWKHWPEVRERFIHRKKILLLLDFDGTLSPLVKNPNKAKLP
                     RRLRTSLRELRDMPRVRVAIMSGRPLSYLKSEIGLRRVHYGSNHGLEITGPALSFRHS
                     AAAAARPLLQRLARRVKRALRSVPGSHLEDKGLTLSLHYRAVPAPHRKAFFGILRRAK
                     SRTAGLPLLWTSGRKVWELRPKLLWDKGKAGLHLVRRLGNPFPIVIGDDRTDEDMFAS
                     LRERGLAIRVGRGKGSAAEFYLRRQTDVLRFLNAVRRDLSARREP"
     gene            1320..>1565
                     /locus_tag="P5079_10475"
     CDS             1320..>1565
                     /locus_tag="P5079_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HRY30445.1"
                     /translation="MIPRAEKPFHFYTRLSLPLLTGIKVRNLRELAEHIKTAPESVIY
                     QHTHRYLQQHQYLVPEPSNDFAYWVSSVLQDDELGEKL"
     assembly_gap    1566..1575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1576..1969
                     /locus_tag="P5079_10480"
     CDS             <1576..1969
                     /locus_tag="P5079_10480"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HRY30446.1"
                     /translation="DELRRAVVETIEAHLSRKGDLRQLPEGKEFHFMGTVRFSVPTRY
                     QAYDLAEFADGLRRVSVSSLYLHIFEARLRPPLGLNDFSLWFDSELGEAALAKKVSSL
                     DPYTQTLEGLRARIIGLVEKRLEVLRHA"
     gene            1962..3185
                     /locus_tag="P5079_10485"
     CDS             1962..3185
                     /locus_tag="P5079_10485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30447.1"
                     /translation="MHNIDAYRPIVGSDVIDEMFLLAKHLEGRSILNVNSTAVGGGVA
                     EILNRMIPLCRDMGLNAQWEVIQGGEDFYAVTKKFHNALHGDPQTVTARDYEVYEETT
                     EENLSRLNLDADVVFIHDPQPAALVRKRKMLRNSWLWRCHIDLSRRDPQVWDFLKPYV
                     EMYDACVFSAPAFAQTLSIPQVLIAPSIDPLSDKNRELPPEEIETILQRLQIPTDKPL
                     VTQVSRFDRLKDPLGVIQAFQKVKPYVEARLILVGGSADDDPEGARVLEEVREKAHAD
                     PDILVLCLPPTSHREINAIQRASTVIFQKSLREGFGLTVSEALWKGKPVIAGAVGGIP
                     LQIAHKYSGILVHSVEGAAYWLKQLLHEPAYAKKLGENGRETVRQNFLITRHLRDYML
                     LFLSAGRLDETMIRL"
     gene            3338..>3807
                     /locus_tag="P5079_10490"
     CDS             3338..>3807
                     /locus_tag="P5079_10490"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRY30448.1"
                     /translation="MAALLKRWDGVKLKPSQWRIPLSQGREALRRLPRERQTSLRDAA
                     RQVERFHRWERRRLGTTWAQTADGLTVGQRIVPVDSVGVYVPGGRFAYPSSVLMAALP
                     AKVAGVRRIVMVTPPRHVTDEVLAAAALAGVTEIYRIGGIGAVGALAYGTRTIP"
BASE COUNT          754 a         1232 c         1130 g          681 t
ORIGIN      
        1 gggaagttcg tcttcgtgca actcggggcg cccagccgca cgcacatccc gcgctaccag
       61 aacttcttaa cggacgtgga ggcgatggcg gaacgcatca acgagaaatt caagggccgg
      121 gattataaac ccatcgtgtt cattgaaaag caccacagcc atcgggagat cgcgcctttc
      181 ttcaaggggg cggacgtctg cctcgtcact tccctccatg atgggatgaa cctggtggcc
      241 aaggaattcg tgtcggcgcg ggacgacaac ggcggcgtct tgatcttgag ccagttcacg
      301 ggcgccgccc gggaactgag ggacgccctc gtcgtgaacc cctacgacat cgaaaaaacg
      361 gcggacgccc tccgggaagc ccttgaaatg ccggcggagg aaaagaccaa tcgcatgaaa
      421 aagatgcggg aaaccgtcaa ggaacacaac atctatcgct gggccgggac actcatcgag
      481 gagttgaccc gggtccggat cggcccggcg ggcggggcgg ggacgtgagg gatgaagaac
      541 ctctggaaac actggccgga agtccgggaa cgtttcattc accggaaaaa aatcctcctc
      601 ctgttggatt ttgacggaac cctttcgccg ctggtcaaga acccgaacaa ggcaaagctt
      661 ccccggcggc tcaggacctc gcttcgcgaa ctccgggaca tgccccgcgt ccgcgtggcc
      721 atcatgagcg gccgtcccct ctcttatctg aaatccgaaa tcggactccg gcgggtgcat
      781 tacgggagca atcacggact cgaaataacc ggcccggcgc tttcgttccg gcattccgcc
      841 gccgccgcgg cccggcccct ccttcagcgg ctggcccggc gagtgaaacg ggccctacgc
      901 tcggttcccg gatcccatct ggaggacaaa gggctgaccc tgagcctcca ctaccgggcc
      961 gtccccgcgc cgcaccggaa ggccttcttc ggaatcctcc gacgggcgaa atcacgcacg
     1021 gccggtctcc cgttgctctg gacctccggc cgtaaagtct gggaactccg ccccaaactc
     1081 ctctgggaca aagggaaagc cggcctccat ctggtccggc gtttgggaaa ccccttcccc
     1141 atcgtcatcg gcgacgaccg cacggacgag gacatgttcg cttccctccg ggaacgcggc
     1201 ctcgccatcc gcgtggggcg cggcaaaggt tcggccgccg aattctatct gcgccgccag
     1261 acggacgtcc tgcgttttct gaacgccgtc cgccgggacc tgtccgcgag gagggaaccg
     1321 tgatcccgcg ggcggaaaaa cctttccatt tctacacccg gctgagcctg cctctcctga
     1381 ccggcatcaa ggtgcgcaac ctgcgggaac tggcggaaca catcaagacc gccccggaat
     1441 cggtgatcta ccagcacacg caccgctatc tgcagcagca tcaatacctc gtgccggaac
     1501 cctccaacga tttcgcctac tgggtctcgt cggtgctcca ggacgacgag ttgggggaaa
     1561 aactcnnnnn nnnnncgacg aactgaggcg ggccgtcgtc gagaccatcg aggcgcatct
     1621 ctcccgcaaa ggcgatctcc gtcagctccc ggagggcaag gagttccact tcatggggac
     1681 ggtgaggttc tccgtcccca cccgttatca agcgtatgat ctggccgaat tcgccgacgg
     1741 gctccgccgg gtcagcgtct ccagcctgta tctgcacatc ttcgaggccc ggctccggcc
     1801 cccgttgggc ctcaatgatt tctccctgtg gttcgactcc gaactcgggg aagccgccct
     1861 cgccaaaaaa gtgtcgtctc tggatcccta cacccaaacg ctcgaaggac tccgcgcccg
     1921 gatcatcggc ctggtggaga aacgcctgga ggttctgcgc catgcataac atcgacgcct
     1981 accggccgat cgtcggctcg gacgtgatcg acgaaatgtt cctcctggcg aaacatctgg
     2041 aagggcggag cattctgaac gtcaactcca cggcggtcgg cggcggggtg gccgaaatcc
     2101 tgaaccggat gatcccgctg tgccgtgaca tgggactgaa cgcccaatgg gaggtcatcc
     2161 agggcggcga ggatttctat gcggtgacga agaaattcca caacgccctg cacggcgacc
     2221 cgcagaccgt caccgcgcgg gattacgagg tctacgagga aacgacagag gagaaccttt
     2281 cgcggctcaa cctggacgcc gacgtcgtct tcatccacga ccctcaaccc gccgctctgg
     2341 tccgaaaacg gaagatgctg aggaattcgt ggttgtggcg gtgccacatc gatttgtccc
     2401 gccgggatcc gcaggtgtgg gacttcctga aaccctacgt cgaaatgtac gacgcctgcg
     2461 tcttctccgc gcccgctttc gcgcagacct tgtccatccc ccaagtcttg atcgccccgt
     2521 ccatcgaccc cctcagcgac aagaaccggg aactcccgcc ggaagagatc gaaaccatcc
     2581 tacagcgcct ccaaattccg accgacaagc ctttggtgac ccaggtttcc cgtttcgacc
     2641 gtctcaaaga tcctctcggc gtcatccagg ccttccagaa ggtcaaaccc tacgtggagg
     2701 cccggttgat cctggtgggc ggctcggccg acgatgatcc ggagggcgcc cgcgtgctgg
     2761 aggaggtgcg ggaaaaagcc cacgccgacc cggatatcct cgtgctttgt ctgccgccga
     2821 cgagccatcg cgaaatcaac gcgatccagc gagcctccac cgtcattttt cagaaatctc
     2881 tccgggaggg gttcgggctg acggtgtcgg aggcgctctg gaaaggcaaa cccgtcatcg
     2941 cgggagcggt gggcggcatt ccgctccaga tcgcccacaa atattccggg atcctggtgc
     3001 attccgtgga aggggcggcc tactggctca aacagctctt gcatgaaccg gcctatgcca
     3061 aaaaactggg ggaaaacggc cgtgaaaccg tccggcagaa tttcctgatc acccgccatc
     3121 ttcgggacta tatgcttttg tttttgtccg ccggacgcct ggatgagacc atgatccgtt
     3181 tgtagctttt ggggcccgaa acaccccctc tggccggcga acgacttccc accgttatca
     3241 tcgaaaaatc aacctaattt tgatacaatt cacacttacc ttggaacaaa aagtccgcac
     3301 tattttaggg gatatacggc gaaaggggga cctcgcggtg gccgcccttt tgaaacgttg
     3361 ggacggggtg aaattgaaac cgtcccagtg gaggattcct ttgtcgcagg ggcgagaggc
     3421 cctgcgccgc ctgccccgcg aaagacagac ctccctgcgg gacgccgccc gccaggtgga
     3481 gcggtttcat cgttgggaac gacgccgcct gggaacgacg tgggcgcaaa cggccgatgg
     3541 actcaccgtg ggccaacgga tcgttccggt ggattccgtc ggggtctacg tgcccggggg
     3601 acggttcgcg tatccttcca gcgtcttgat ggcggccctc cccgccaagg tggcgggcgt
     3661 ccggcgcatc gtgatggtga ccccgccacg gcacgtcacg gacgaggtct tggcggcggc
     3721 cgcgctggcc ggggtcaccg aaatttatcg tatcggcggc atcggcgcgg tcggcgccct
     3781 ggcctacggc acccggacca tcccgaa
//
LOCUS       DAOTFD010000241         3800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_17891_length_3800_cov_4.812283, whole genome shotgun sequence.
ACCESSION   DAOTFD010000241 DAOTFD010000000
VERSION     DAOTFD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17891_length_3800_cov_4.812283"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="P5079_10495"
     CDS             <1..319
                     /locus_tag="P5079_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30449.1"
                     /translation="KGFAVQKLATEAEAEEHLKNNRPDIVVMGLMLEYPDSGFILSHR
                     IKQKDPALPIIMVTSVARKMGFSFEAATEEERSWVKADVVMDEPVRFEQLWNEAEKLL
                     RRN"
     gene            375..1805
                     /gene="hydG"
                     /locus_tag="P5079_10500"
     CDS             375..1805
                     /gene="hydG"
                     /locus_tag="P5079_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRY30450.1"
                     /translation="MSAAPHGRSSKTAPRKEFAVRADLIRSVLRDRRAPDPARVRDIL
                     AKAGELHGLGWDDVAALSAVTDPALLQSLFDAARRVKETIYGRRLVLFAPLYISNLCH
                     NECLYCAFRSRNRSLRRRSLTLDEVERETRVLIDQGHKRLLVVAGETASDAYLDGVLA
                     AIERIYSVRHGQGEIRRVNVNIAPLTVDQFRRLLAAHIGTYQLFQETYDREVYERVHL
                     RGQKADYEGRLGAMDRAMEAGFGDVGIGVLFGLADWRFELLAVLEHIAHLEKKFGVGP
                     HTISVPRLEPAVGSAMSANPPTPVSDADFLKIVAILRLAVPYTGLIMSTRESPEVRRQ
                     TFALGVSQISAGSRTNPGGYAENADGADAEQFSLGDHRPLDEVIRDIAGLGYVPSFCT
                     ACYRLGRTGRDFMDLAKPGDIKRHCDPNALSTFLEYLLDYASPDTLARGEDAIRRQLD
                     DMDPAARRRAETMISKVRSGRRDVFC"
     gene            1805..3052
                     /gene="hydF"
                     /locus_tag="P5079_10505"
     CDS             1805..3052
                     /gene="hydF"
                     /locus_tag="P5079_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRY30451.1"
                     /translation="MMNTPKGFRLHIGLFGRRNVGKSSLLNAVTRQEVSIVSETAGTT
                     TDPVEKPMEFLPLGPVLFIDTAGVDDEGALGELRIAKTRTVFDRTDLAVVVSDGAWGD
                     YEQKLLDEFKSRAVPVLVVFNKFDLRPPDPGLLDRLEESKIPFVSTSAPRGEGLLEFR
                     QALLDNAPADFINSPRIIGDLIEPGDLVILVIPIDKEAPKGRVILPQVQTLRDVLDSH
                     ASALVLQETELKEAFKNLPRRPRLVVTDSQAFEKVAADTPPDVWMTGFSVLFSRFKGD
                     LAVQTEGALAIDRLRPGDRVLVAESCSHHPIADDIGREKIPRWLQKKAGGPLRFHTVQ
                     GHDFPPDLADYKLVVQCGACMGNRREVLSRIMRCRAAGVPFTNYGLAIAYCLGLFQRA
                     LEPFPQALEIYRRAVASGQKVSS"
     gene            3049..>3800
                     /locus_tag="P5079_10510"
     CDS             3049..>3800
                     /locus_tag="P5079_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30452.1"
                     /translation="MNRPEILRWLREGRPSDLKTLWSRADAARRKNVGDAVHLRGLVE
                     ISNHCVRSCAYCGINRAHRGITRYRMTAHEVLACARQAVGLGYGTVVLQAGEDPGLRR
                     AAVADLVRRIKKDTGLAVTLSLGERDDGDLVAWKQAGADRYLLRFETSNPDLYARIHP
                     SLPGRPSDRFALLRRLRALGYEVGSGVMIGIPGQTYESLADDIETFARLDLDMIGMGP
                     YIAHPSTPLGVDAAGGMAASSAAVDAGDEEAAA"
BASE COUNT          614 a         1478 c         1116 g          592 t
ORIGIN      
        1 aaaggggttc gccgtccaga agctggccac cgaggccgag gcggaggaac acctcaagaa
       61 caaccgcccg gacatcgtgg tcatggggct catgctggaa tatcccgaca gcggcttcat
      121 cctcagccat cgcatcaaac agaaggaccc cgccctgccc atcatcatgg tcacctccgt
      181 cgcccgcaag atgggctttt ccttcgaggc ggccaccgag gaggaacgct cctgggtcaa
      241 ggccgacgtg gtcatggacg aaccggtgcg gttcgaacag ctttggaacg aggctgaaaa
      301 acttctcagg aggaactgac gccgggcccc ggccggaacc tctccggttt cgcccgcccg
      361 gccgtcgtca tgccatgagc gccgccccgc acggtcgttc gtccaagacc gccccccgaa
      421 aggaattcgc cgtccgcgcg gacctcatcc gttccgtgct gagggaccgg cgcgccccgg
      481 acccggcccg cgtccgcgac atcctcgcca aggcgggcga actccacggc ctcggctggg
      541 acgacgtggc cgccctctcc gccgtcacgg accccgccct ccttcagtcc ctcttcgacg
      601 ccgcccggcg ggtgaaggag accatctacg gccgccggct cgtcctcttc gcgcccctct
      661 acatctccaa cctctgccac aacgagtgcc tctactgcgc cttccgctct cggaaccgct
      721 ccctgcgccg ccggtccctc acgctcgacg aggtggaacg cgagacccgg gtgctcatcg
      781 accagggcca caagcggctc ctcgtcgtgg ccggcgaaac cgcctccgac gcgtatctgg
      841 acggcgtcct cgccgccatc gagcgtatct attccgtgcg ccacggccag ggcgagatcc
      901 gccgcgtcaa cgtcaacatc gccccgctca cggtggacca gttccggcgc ctcctcgccg
      961 cccacatcgg cacctaccag ctttttcagg agacctacga ccgcgaggtg tatgagcgcg
     1021 tgcacctccg cggccagaag gccgattacg aaggacgcct cggcgccatg gaccgcgcca
     1081 tggaggccgg cttcggcgac gtgggcatcg gcgtcctctt cggcctggcc gactggcgct
     1141 tcgagctgtt ggccgttctg gaacacatcg cccatctgga aaagaagttc ggcgtggggc
     1201 cgcacaccat cagcgtgccc cgcctggaac ccgccgtcgg ctccgcgatg tcggcgaacc
     1261 cgcccacgcc cgtctccgac gcggatttcc tcaagatcgt cgccatcctc cgcctggccg
     1321 tgccgtacac cgggctcatc atgtccaccc gcgaatcgcc cgaggtccgc cgccagacct
     1381 tcgccctggg cgtctcccag atctccgccg gcagccgcac caaccccggc ggctacgccg
     1441 agaacgccga cggcgccgac gccgaacagt tctccctggg cgaccatcgc ccgctcgacg
     1501 aggtcatccg cgacatcgcc ggactcggct acgtcccgtc cttttgcacc gcctgctacc
     1561 ggctgggccg caccggccgc gatttcatgg acctcgccaa acccggcgac atcaaacgcc
     1621 actgcgaccc caacgccctc tccaccttcc tggaatatct gctggactac gcctctcccg
     1681 acaccctcgc ccggggggaa gacgccatcc gccgccagct cgacgacatg gaccccgccg
     1741 cccgccgccg cgcggagacc atgatctcca aagtgcgctc cggccggcgc gacgtcttct
     1801 gctgatcatg aacacgccca agggcttccg cctccacatc ggcctcttcg gccgccgcaa
     1861 cgtgggcaag tccagcctcc tcaacgccgt cacccgccag gaggtctcca tcgtctccga
     1921 gaccgccggc accaccaccg accccgtgga aaaacccatg gagttcctgc ccctgggccc
     1981 cgtgctcttc atcgacaccg ccggcgtcga cgacgagggc gccctgggcg aactccgcat
     2041 cgccaagacc cgcaccgtct tcgaccgcac cgacctggct gtggtcgtca gcgacggcgc
     2101 gtggggggac tacgagcaaa aactattgga cgagttcaag tcccgcgccg tgcccgtcct
     2161 cgtcgtcttc aacaaattcg acctgcgccc gccggacccc ggcctcttgg accggctgga
     2221 ggagtcgaag atcccctttg tttccacctc ggccccgcgc ggcgaagggc tcctcgaatt
     2281 ccgacaggcc ctcctcgaca acgcccccgc cgatttcatc aacagccccc gcatcatcgg
     2341 cgacctgatc gagcccgggg accttgtgat cctggtcatc cccatcgaca aggaggcccc
     2401 caaaggccgc gtcatcctgc cccaggtgca gacgctccgc gacgtgctgg actcccacgc
     2461 ctcggcgctg gtgctgcagg agacggaatt gaaggaggcg ttcaaaaacc tgccgcgccg
     2521 tccgcgcctg gtggtcaccg attcccaggc cttcgaaaag gtcgcggccg acaccccgcc
     2581 ggacgtgtgg atgacgggtt tttccgtcct gttctcccgc ttcaagggcg acctggccgt
     2641 ccagaccgag ggcgccctgg ccatcgaccg cctgcgtccc ggcgaccgcg tgctggtggc
     2701 ggaatcctgc agccaccacc ccatcgccga cgacatcggc cgcgagaaga tcccgcgctg
     2761 gctccagaaa aaggccggcg gaccgctccg cttccatacc gtgcagggcc acgatttccc
     2821 gcccgacctc gccgactaca agttggtggt gcagtgcggc gcctgcatgg ggaaccgccg
     2881 cgaagtgctc tcgcgcatca tgcgctgccg cgccgcgggc gtgcccttca ccaactacgg
     2941 cctggccatc gcctactgcc tcgggctgtt tcaacgcgcc ttggaaccgt tcccccaggc
     3001 cctcgagatt taccgtcggg ccgtggcgtc cggccaaaag gtgtcgtcat gaaccggccg
     3061 gagatcctcc gctggctccg cgagggacgg ccgtccgacc tgaaaacgct ttggtcccgg
     3121 gccgacgccg cccgccgaaa aaacgtgggc gacgccgtcc atctgcgcgg cctggtggag
     3181 atctcaaacc attgcgtgcg ctcctgcgcc tattgcggca tcaatcgcgc tcatcgcggc
     3241 atcacccgct accgcatgac cgcccacgag gtcctggcct gcgcccgcca agccgttggg
     3301 ttgggctacg gcaccgtggt cctgcaggcc ggcgaggacc ccggcctccg ccgcgccgcc
     3361 gtcgccgacc tcgtgcggcg cattaaaaaa gacaccggcc tggccgtcac gctcagcttg
     3421 ggcgaacggg acgacggcga cctcgtcgcc tggaaacagg ccggcgccga ccgatacctt
     3481 cttcgcttcg agacctcgaa tccggacctc tacgcccgca tccatccgtc cctgcccggc
     3541 cggccgtcgg accggttcgc cctcctgcgc cgcctccgcg ccttgggcta cgaggtcggc
     3601 agcggcgtca tgatcggcat ccccggccag acctacgaga gtctggccga cgacatcgag
     3661 accttcgccc ggctggacct ggacatgatc ggcatgggcc cttacatcgc gcatccatct
     3721 acgccgctgg gcgtagacgc ggcgggtggc atggccgcct cgtcagcggc cgtggacgcc
     3781 ggagatgaag aagccgccgc
//
LOCUS       DAOTFD010000242         3744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_18334_length_3744_cov_3.481431, whole genome shotgun sequence.
ACCESSION   DAOTFD010000242 DAOTFD010000000
VERSION     DAOTFD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18334_length_3744_cov_3.481431"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="murD"
                     /locus_tag="P5079_10515"
     CDS             complement(<1..1115)
                     /gene="murD"
                     /locus_tag="P5079_10515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRY30453.1"
                     /translation="MDLFAHPVLSAWFRRKEVCVVGLGKSGLAAAAFLKRLGARVSVT
                     EKQPRQKAAKWVRELPPGVFCETGSHRLLRRSWDLLVISPGVPAEVWKPHAAAGRMVW
                     GEMELGYRALSLAGRWPRRAAAVTGTNGKTTTTALLGEICRAAGFHTVVAGNIGTPLC
                     AVAHRLDAGAALVLEVSSYQLETACAFHPAAGVVMNVTPDHLKRHRTMKNYAEAKFRL
                     FQNFLPEETAVLNAADPWCRRLARTVPGKIFWFGRAAERRGRPGVFFDGKRLRAAVPG
                     GRAVWDPPRYLRGYHNVENAMAAAACGLALGAGGAAVRKSFERFRGVEHRLEEVRCRN
                     GVHFVNDSKATNVDSTAVALRSFVGGLHLILGGQDKGS"
     gene            complement(1117..2205)
                     /gene="mraY"
                     /locus_tag="P5079_10520"
     CDS             complement(1117..2205)
                     /gene="mraY"
                     /locus_tag="P5079_10520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRY30454.1"
                     /translation="MTYYLHYLREFFSPLNVFQYITFRAGGAFLTSLLIWLFAGKWFI
                     NFLKNKKIQQSIREYGPSTHLAKAGTPTMGGLLILVAMVFSTLLWARVDNRFIVLTLL
                     SAVYLGAVGFMDDYRKWLTKHPAGGLSDSVKMGAQVALALGVAVYLYFCPPNASYGTW
                     VDVPYLKHVYLTLGNVYILFALFVIVGTSNAVNIADGLDGLAGGTLLVSAITFGIFSY
                     LAGHVKLSEYLRIVTVPGAGELTVYLAAMAGACLGFLWYNAYPAEIFMGDTGSLFMGG
                     TIGIAALCIKQELILVLVGGIFVAEAVSVILQVGSVRYRGGKRVFRMAPLHHHFEVGG
                     MPETKVTMRFWIVAVVLALLALTSLKIR"
     gene            complement(2219..3601)
                     /gene="murF"
                     /locus_tag="P5079_10525"
     CDS             complement(2219..3601)
                     /gene="murF"
                     /locus_tag="P5079_10525"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRY30455.1"
                     /translation="MKPAWRMDDVARLCGGQVMSGDPKTELGPIVFDSRAVEPGVTFL
                     ALKGHRQDGHDFVSEAVARGASAVIVSRECPLPSWPGVCVRVSDSLSALQALGAAHRD
                     RLGLRVIGVTGSNGKTTTKEMVSHVLTRAGNSVFSSKGNLNSQLGLPLMLLEMDDSHE
                     YGVLEMGASQRGEIARLASLAKPQVGVITNAGLAHLEFFGSAEGVAEAKWELAAALPA
                     TGAAVINADDPALAARARGLKCSVIRYGTAAGADVRAENIRPGPETVFDLTVGFFRQE
                     VRLSTPGRFNVYNALAAVAVGMWEKVDMKTLVRALETFSPPPSRMEVRTRRDGAVFVL
                     DAYNANPSSMKASLESFVSAYPSRRKIAVLGGMKELGAFMEKEHEALGALLAALSLDE
                     VFFLGEEADWVRRGFERGKGAAAFRGFGNKEALHAGLRGRLSGGTAVLFKASRSVALE
                     NIYGPLLTEK"
BASE COUNT          748 a         1346 c         1056 g          594 t
ORIGIN      
        1 gaccccttgt cctggccgcc caggatcaga tgcagccctc cgacgaaaga acgcaaagcc
       61 accgcggtgg aatccacgtt cgtcgccttg gaatcgttca cgaaatggac cccgttccgg
      121 cagcgcacct cttcgaggcg gtgttccacg ccgcgaaacc gttcgaaact tttccggacc
      181 gccgcccctc cggcgccgag ggccagcccg caggccgccg cggccatggc gttctccacg
      241 ttatgataac cgcgcagata acgcggcgga tcccagaccg ccctccctcc gggaacggcg
      301 gcccgcagcc gttttccgtc gaagaaaacg cccggccggc cgcggcgctc cgccgcgcgg
      361 ccgaaccaaa atatcttccc cggcaccgtg cgcgccagac ggcgacacca cggatcggca
      421 gcgttcagca ccgcggtctc ctccggcaga aagttttgga acagccggaa cttggcctcg
      481 gcgtagtttt tcatggtgcg gtgccgtttc aaatggtcgg gcgtcacgtt catcaccacg
      541 ccggcggcgg gatggaaagc gcaggccgtc tccagttgat agctggacac ctccaacacc
      601 agcgcggcgc ccgcatccaa ccgatgcgcc acggcgcaga gaggcgttcc gatgttcccc
      661 gccaccaccg tgtggaaccc cgcggcgcgg cagatctccc cgagcagagc cgtggtggtg
      721 gtttttccgt tggtgccggt caccgcggcg gcccggcgcg gccaacggcc cgccagggaa
      781 agcgcgcggt atcccaactc catctctccc cataccatcc ggccggccgc ggcgtggggt
      841 ttccacacct ccgccggcac gcccggactg attaccaaga ggtcccacga ccgacgaaga
      901 agacgatgcg atccggtctc gcaaaaaacg ccgggcggca actcccggac ccatttcgcc
      961 gctttctgac gcggctgttt ttccgtgacg ctcacccggg cgccgagccg cttcaaaaac
     1021 gcggccgccg ccagccccga cttgcccagc cccaccacac acacctcctt gcggcgaaac
     1081 caggcggaaa ggacggggtg agcgaacaga tccatgttac cggatcttca gggaggtgag
     1141 ggccaggagg gccaacacca ccgccacgat ccaaaaccgc atggtgacct tggtctccgg
     1201 catccctccc acttcaaagt ggtgatgcaa cggcgccatg cggaagaccc gtttccctcc
     1261 ccggtaacgc accgacccca cctgcaggat gacggacacc gcctccgcca cgaagatccc
     1321 tcccaccagg accaggatca actcctgttt tatgcagagc gcggcgatgc cgatggtgcc
     1381 gcccatgaag agggagcccg tgtctcccat gaaaatctcc gcgggatagg cgttgtacca
     1441 aagaaaaccc aggcacgcgc ccgccatggc cgccagatac accgtcaatt ccccggcgcc
     1501 gggcacggtg acgatgcgca gatattcgga cagtttgacg tgtccggcca gataggaaaa
     1561 aatcccgaaa gtgatggcgg agaccagaag cgtccctccg gccaacccgt ccagcccgtc
     1621 ggcgatgttc accgcgttgg aggtgccgac gatgacgaac agggcgaaaa ggatatagac
     1681 gttccccagc gtcagataca cgtgcttcag atacggcaca tccacccagg tgccataact
     1741 cgcgttgggc ggacaaaaat acagatagac cgccaccccc aacgccaacg ccacctgcgc
     1801 gcccatcttc acggagtcgg acagcccccc ggcgggatgc ttcgtcagcc atttccgata
     1861 atcgtccatg aaacccaccg cgccgagata gacggcggag aggagcgtca acacaataaa
     1921 ccggttgtcc acccgcgccc acagcagcgt ggagaaaacc atcgcgacga ggatcaagag
     1981 gccccccatg gtcggcgtgc cggccttggc cagatgcgtg gaggggccgt attcccgtat
     2041 ggactgctgt atctttttgt tcttcaagaa attgatgaac catttcccgg cgaaaagcca
     2101 gatcaacagc gaggtcagga acgcgccgcc ggcgcggaag gtgatgtatt ggaacacgtt
     2161 caacggcgag aaaaattcgc gcagatagtg caggtagtag gtcatgtgtc tcccctcttc
     2221 atttctccgt caacagcgga ccgtagatgt tctccaacgc cacgctccgc gacgccttga
     2281 acagaaccgc cgtcccgccg gacaacctcc cgcgcaaacc ggcatgcaat gcttccttgt
     2341 tcccgaatcc ccggaacgcg gcggctccct tcccgcgttc gaaaccccgc ctcacccagt
     2401 ccgcctcttc gcccaagaaa aagacctcgt ccaacgacag ggccgccagc agcgcgccca
     2461 gcgcttcgtg ttccttctcc atgaacgcgc ccagttcctt catgccgccc aacaccgcga
     2521 ttttccgccg ggaaggatag gccgacacga aactttccag cgacgccttc atggagctgg
     2581 ggttggcgtt gtaggcgtcg aggacgaaca ccgcgccgtc tcgtcgcgtg cgcacctcca
     2641 tgcggctcgg cggcggcgaa aaagtttcca gggctcgcac cagtgttttc atgtccactt
     2701 tttcccacat gcccaccgcc accgcggcca aggcgttgta gacgttgaac cgtcccgggg
     2761 tcgagagcct cacttcctga cggaaaaatc ccaccgtcag gtcaaaaacg gtttccggcc
     2821 ccggacggat gttctccgcc cgcacgtcgg ccccggcggc ggtgccgtag cggatgacgg
     2881 aacacttcaa gccgcgcgcc cgggccgcca gggccgggtc atcggcgttg atcacggccg
     2941 cgccggtggc cggcagggcg gcggccaatt cccatttcgc ctcggccacc ccttccgcac
     3001 tcccgaaaaa ttccagatgc gccaaccccg cgttggtgat cacgcccacc tgcggcttgg
     3061 ccagggacgc caaccgcgcg atctcccccc tctgcgaggc gcccatctcc agcaccccgt
     3121 attcgtgaga gtcgtccatc tccagcaaca tcaacggcag gccgagttgg ctgttgaggt
     3181 tccccttcga cgaaaaaacc gagttccccg cccgcgtcag gacatgcgac accatctctt
     3241 tggtggtggt cttcccgttg ctgccggtca ccccgatcac ccggagaccc agccggtcgc
     3301 ggtgggcggc cccaagcgcc tgcagggcgg aaagggaatc ggacacacgc acgcacaccc
     3361 ccggccatga cggcaacgga cattcccggg aaacgatcac cgcggacgcc ccgcgcgcca
     3421 ccgcctccga cacgaaatcg tgcccgtcct gccggtggcc tttcaatgcc agaaaagtga
     3481 cgccgggctc cacggcgcgc gaatcgaaaa cgatcggccc cagctcggtc ttcgggtccc
     3541 ccgacatcac ctgtccgccg cacagacgag ccacgtcgtc catgcgccat gccggtttca
     3601 tgaggagaat cctcccagac ccgaacccct acgcgccgac ccggccggaa agcgcctgct
     3661 gaaccgccac gcggtcgtcg aaatcgacgg tcctgtcttt gaaaatctga taggtctcat
     3721 gtccctttcc cgccaccagg acga
//
LOCUS       DAOTFD010000243         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_18427_length_3730_cov_3.981769, whole genome shotgun sequence.
ACCESSION   DAOTFD010000243 DAOTFD010000000
VERSION     DAOTFD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18427_length_3730_cov_3.981769"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="P5079_10530"
     CDS             complement(<1..720)
                     /locus_tag="P5079_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRY30456.1"
                     /translation="MSNRFTERAQRVILIAQEEAKRLNHDYVGTEHLLLGLIALGEGV
                     AAQVLANLGVDLRRVRSEIEKIVGTGDNVMLLGEIPFTPRAKKVLELAVEEAQNMGHN
                     YVGTEHLLLGLIREEEGVAARVLENIGVRLDVVREEVISLLGEGHVAQPQQQHAPASK
                     SKSKTPTLDEFGRDLTQLAREAKLDPVIGRENEIERVIQILLRRTKNNPVLIGDPGVG
                     KTAIVEGLAQKLASDEAPELLT"
     gene            complement(960..1874)
                     /gene="ilvE"
                     /locus_tag="P5079_10535"
     CDS             complement(960..1874)
                     /gene="ilvE"
                     /locus_tag="P5079_10535"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRY30457.1"
                     /translation="MKTKASSSKTAPQKLIYLNGQLVPEKEAKISVFDHGLLYGDGVF
                     EGIRAYKGRVFRLREHMQRLYHSARAITLDVKMPMEELEQKVLDTLRANKLRDAYVRL
                     VVTRGTGDLGLDPRKCRKPTVFIIANEITLYPESCYKNGLEVITASTRRNSPQALSPN
                     VKSLNYLNNILAKIEANLGGVPEAILLNLEGYIAECTGDNIFYIRGRNLYTPPIAAGA
                     LPGITRECVLKLGADLGLSPEEKLFTSYDLYVADEVFLTGTAAEVIPVVKLDSRVIGS
                     GKPGPLTQKLIRAFRDLTSREGVPITPA"
     gene            complement(1890..2561)
                     /locus_tag="P5079_10540"
     CDS             complement(1890..2561)
                     /locus_tag="P5079_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY30458.1"
                     /translation="MSAQPVVEVQDLHKRFDTLEVLKGLNFRVAPGEIICVWGPSGAG
                     KSTLLHVLGLMTPPSEGKLNLFGQDMARPADSALSKIRNERIGFLFQFHYLLPDLSLL
                     ENVMIPLLIRGEPRESAEGKAKKILERLGLGPRLHHRPAQSSGGEQQRTALARALVGE
                     PALLLADEPTGNLDRGIGREVEKLLKSEVKSRGTTLVLVTHDETLAAEADRRLVLIDG
                     VLNAG"
     gene            complement(2566..>3730)
                     /locus_tag="P5079_10545"
     CDS             complement(2566..>3730)
                     /locus_tag="P5079_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY30459.1"
                     /translation="GNLTSVIAISCVAVGVAALVVTLAVMTGFREDIRGKILGAQPHL
                     VVMGSQGSLAAGDWSDVFLDVPDVQAWAPFVLGQALIRKGSTTLGVVVKGVEPEKESQ
                     VTQLDKKLVDGRWDELAAPTREGVPVFLGKELARSLNVSIGDEVLIAVPTPQTAVMSG
                     LPSLFPFKVAGILETGLYDYDSSLAVTTLPAAQTMFQMPKDYSGLGVRLKDPDDSLGA
                     AMRVQKNIGSAAWVRSWLALNRNLFAALKLEKIVMFIVLTLITLVAAFTIVSNLLLVT
                     AQRVHEIGILRAMGATRGAIQRIFLLKGLLMGTLGTAVGVGLGLALSLVLKRYQFIKL
                     PADVYYVETLPVKIMPSDVGLVAGAALLIVLIATLYPSRLAAKLDALSAIRQL"
BASE COUNT          659 a         1138 c         1193 g          740 t
ORIGIN      
        1 cgtcagcagt tcgggcgctt cgtcggaggc gagcttttgc gccaggcctt ccacgatggc
       61 ggtttttccc acgcccgggt cgccgatcag caccgggttg ttcttggtgc gccgcaggag
      121 gatctggatg acgcgttcga tctcgttctc ccggccgatg acgggatcga gtttcgcctc
      181 ccgggccaat tgggtcaggt cccggccgaa ctcgtccagg gtgggtgttt tggatttgct
      241 tttggaggcc ggggcgtgct gttgctgggg ttgcgccacg tggccttctc ccaagaggga
      301 gatgacctct tcgcgcacca cgtcgaggcg tacgccgatg ttctccagca cccgggcggc
      361 cacgccctct tcttcgcgga tgaggccgag caggaggtgt tcggtcccca cgtagttgtg
      421 acccatgttc tgggcctcct ccaccgccag ctccaaaact tttttcgccc gcggcgtgaa
      481 agggatctcc cccagcaaca tgacgttgtc gccggtgccc acgatctttt caatctcgct
      541 ccgcacgcgg cgcagatcca ccccgaggtt cgccaacacc tgggcggcca cgccctctcc
      601 caacgcgatg aggcccagaa gcagatgttc cgtcccaacg tagtcgtggt tcaaccgttt
      661 ggcctcttct tgggcgatga gaatcacccg ttgagcgcgt tctgtaaagc gatttgacat
      721 aactccctct ccggttcgtt ttgcctgcaa aaactatttt aaaccgtttt ccatgcggtg
      781 tcaatcaaaa atcaacgatt ttcagcggga cattgaagaa aacgctctct cgggacaaaa
      841 gttcccttgt tactattttg aagcaatttt ccttaaggaa gaattcttaa gggcggatta
      901 agacaaaaaa acactctccg gctttcgccg gccgaaggac gcagggcggg gacggaggat
      961 caggccggcg tgatgggcac gccttcgcgc gaagtgaggt cgcggaaagc gcggatgagt
     1021 ttctgggtga gcggccccgg ttttcccgac ccgatgacgc gggaatcgag tttgaccacg
     1081 gggatgacct cggccgcggt gccggtgagg aacacctcgt cggccacgta gaggtcgtag
     1141 gaagtgaaga gtttctcctc cggcgacaaa ccgaggtcgg cgccgagttt cagaacgcac
     1201 tcgcgggtga tgccgggcag ggcgccggcg gcgatcggcg gggtatagag gttccgtcca
     1261 cggatgtaga agatgttgtc cccggtgcat tcggcgatat agccttccag gttcagcagg
     1321 atggcttccg gcacgccgcc gagattggcc tcgatcttcg ccaggatgtt gttgaggtag
     1381 ttcagcgatt tcacgttggg gctgagggcc tgcgggctgt tgcggcgcgt ggaggcggtg
     1441 atgacctcca gcccgttctt gtagcaggat tccggataga gggtgatctc gttggcgatg
     1501 atgaacaccg tcggtttccg gcatttccgg ggatccagcc ccaggtcgcc ggtgccgcgc
     1561 gtcaccacga gccgcacgta cgcgtcgcgc agtttgttgg cgcgcagggt gtccagcacc
     1621 ttctgttcga gttcctccat cggcatcttc acgtccagcg tgatggcccg ggcggagtgg
     1681 tagaggcgct gcatgtgctc gcgcagacgg aacacgcgcc ccttgtaggc gcggatgcct
     1741 tcaaacacgc cgtcgccgta gaggaggccg tggtcgaaca ccgaaatctt cgcctctttt
     1801 tcagggacca attggccgtt gagatagatg agtttttgcg gagccgtttt ggacgaggat
     1861 gccttcgttt tcactgcgcc tccttgtgtt taaccggcgt tcaaaacgcc gtcgatcaga
     1921 accagccgcc ggtcggcttc cgccgccagc gtttcgtcgt gggtcaccag gaccagcgtg
     1981 gtgccgcgcg atttgacctc gctcttcaac aatttttcga cttcccggcc gatgccgcgg
     2041 tccagattcc ccgtgggctc atcggccaag agcagcgcgg gttcgcccac cagggcccgc
     2101 gccagagccg tccgctgttg ttccccgccg gaagactgcg ccggccggtg atgcagccgc
     2161 gggcccaggc ccagccgctc gagaattttt ttcgcttttc cttcggcgga ttcgcggggt
     2221 tcaccgcgga tcaacagcgg tatcataaca ttttctaaca gggaaaggtc gggcaggagg
     2281 tagtggaact ggaagagaaa tccgatgcgt tcgttgcgga ttttggagag ggcggagtcc
     2341 gcgggacggg ccatgtcctg gccgaaaagg ttcagtttcc cctcgctcgg cggcgtcatc
     2401 aggcccaaca cgtgcagcag cgtggatttt cccgcgcccg acggacccca gacgcagatg
     2461 atttcgccgg gggccacccg gaaattcaat cccttcaaca cctccagggt gtcgaaacgt
     2521 ttgtgaaggt cttgcacctc gacgacgggt tgggcggaca tggcgttaaa gttgccggat
     2581 ggcgctcagg gcgtccagtt tggccgccag ccgggaagga tagagcgtgg cgatgagcac
     2641 gatcagcagg gccgctccgg ccaccagccc cacgtccgac ggcatgatct tcaccggcag
     2701 cgtctccacg tagtacacgt ccgccggcag cttgatgaac tggtagcgtt ttaacaccag
     2761 ggagagggcg agtcccaacc ccacgccgac cgcggtaccg agggttccca tcagaaggcc
     2821 tttgaggagg aaaatccgct ggatggcgcc gcgcgtggcg cccatggcgc gcaggatgcc
     2881 gatctcgtgc acgcgttgcg cggtcaccag caacaggttc gacacgatgg tgaacgccgc
     2941 caccagcgtg atcagcgtca gcacgatgaa catcacgatc ttctccagtt tcagggcggc
     3001 gaagaggttc cggttcaacg ccagccagga ccgcacccag gcggcggacc cgatgttctt
     3061 ctgcacccgc atggccgcgc cgagcgagtc gtccgggtct ttgaggcgca cgcccagccc
     3121 gctgtagtct ttgggcattt ggaacatcgt ctgggcggcg gggagcgtgg tcacggcgag
     3181 ggaggagtcg tagtcataca ggccggtctc caggataccg gcgactttga acgggaagag
     3241 cgagggcaag ccggacatca cggccgtctg cggcgtgggg accgcgatca gcacctcgtc
     3301 gccgatggaa acgttcaggg agcgcgccag ttctttcccc aaaaagaccg ggacgccttc
     3361 ccgcgtgggg gccgcgagtt cgtcccagcg gccgtcgacc agttttttgt ccagttgggt
     3421 gacctgcgat tctttttccg gctccacgcc cttgaccacc acgcccaggg tggtggagcc
     3481 tttgcggatg agcgcctgcc cgaggacgaa cggcgcccag gcctgcacgt cgggcacgtc
     3541 cagaaacacg tccgaccagt ctccggcggc cagagaaccc tgcgagccca tcaccaccag
     3601 gtgcggctgc gcgccgagga tcttgccgcg gatgtcctcc cggaagccgg tcatcaccgc
     3661 caacgtcacg acgagcgccg ccacgccgac ggccacgcag ctgatcgcga tgacggaggt
     3721 gaggttgccg
//
LOCUS       DAOTFD010000244         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_18573_length_3710_cov_3.494938, whole genome shotgun sequence.
ACCESSION   DAOTFD010000244 DAOTFD010000000
VERSION     DAOTFD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18573_length_3710_cov_3.494938"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5079_10550"
     CDS             <1..321
                     /locus_tag="P5079_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30460.1"
                     /translation="ATFSTTEDMVDKSELTNDSYSVSVNVEIVRNILYSQIWGSMIST
                     EDDDALNLADRQDTSANIEFTYQVKSNTALTLGAYRNVTKDKIAPVNDTTANGGNARV
                     SYSF"
     gene            437..667
                     /locus_tag="P5079_10555"
     CDS             437..667
                     /locus_tag="P5079_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019553809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY30461.1"
                     /translation="MAKTPAHLAIKNRLNVLRAERRITQEQLAKEVGVTRATIIAIEG
                     EDYNPSLELAFRLARYFDTDVQSLFFIKEGTK"
     gene            688..1260
                     /locus_tag="P5079_10560"
     CDS             688..1260
                     /locus_tag="P5079_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30462.1"
                     /translation="MKTYLYGFFFVIGLGIVFNYCGLDDMRNAVSPLRQVNNVGGFVF
                     ASWMLMTMYLSLKLIFSGSFRDAVLSRITFLKERDEREKLFSGQAAKTTFMLTLAVLF
                     FLLSLSCFEVSVYRVPPEKAINGKTGVLTLGVRLELLKDRSAPAPETFRRDIFSHTDL
                     PISSTALILLLIGWQIASYNLCMRRFGIRS"
     gene            complement(1545..2411)
                     /locus_tag="P5079_10565"
     CDS             complement(1545..2411)
                     /locus_tag="P5079_10565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30463.1"
                     /translation="MKKQIALAVFLAILAAPLTAASNYASLTDEERQKLLEEHYARAL
                     QSFNDADYSRAISYWQKILEIDPEQAAPPGLIKQAREKLSAQLGPNEKELRLVLEAGQ
                     YDAALKKTENLSGLDPSNQRYKLLKTKLENIVEVKPAETGTGKAARLIRKGLSAYLAD
                     TEDPRLALNALRYARDINPQDPSTTRLVGYMETQYPDVAKKETVAPGMSVTENKLFIA
                     LNNIYEGRYDLAVLACNEVLELEPDNVLALKRKGSAYFALNQKDKAREIWALALKITP
                     NDAEIKKFLASK"
     gene            complement(2408..3166)
                     /locus_tag="P5079_10570"
     CDS             complement(2408..3166)
                     /locus_tag="P5079_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY30464.1"
                     /translation="MSGLDFESEEELTDPLAGEFAEGSENQELSQLITLEEKTEESSE
                     KLWMIPFADLMSTLVILFLALFGYAYLGASSTYERALNALQKEIAGKDQKAHLLKQEK
                     ETEVAQALEKYFNEDKLKGLAQVELSAQRIRVSLSNPVLFDSGQATLKSEAQKALREI
                     AGLLKGLPNAVVVEGHTDNVPIRTGPYRDNFELSAARAFSVIAFFIGDGLPPDRFSAY
                     GYGEHRPVTDNATLEGRGKNRRIEINIVRTMEKT"
     gene            complement(3169..>3710)
                     /locus_tag="P5079_10575"
     CDS             complement(3169..>3710)
                     /locus_tag="P5079_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY30465.1"
                     /translation="QPEETRRQMMNDMANQFREKPQSAVESKAEQIIQKVQEEDVTQV
                     LQKKKFAQVETSEERIRITLTNPVLFLSGEAVLSDGARREIRDLAPLLASLPNKILVE
                     GHTDNQPVRQKQFASNWELSVARASSVVEFFTRECGIPSERFILAGYGEYRPVSDNDT
                     PDGRRHNRRIEINILRKAA"
BASE COUNT          694 a          949 c         1164 g          903 t
ORIGIN      
        1 gccaccttct ccaccaccga ggacatggtg gacaagtccg aattgacgaa cgattcctat
       61 tccgtgtccg tcaacgtgga gatcgtccgg aacatcctgt attcccagat ctggggcagt
      121 atgatatcga cggaagacga cgatgcgctg aacctggccg accggcagga taccagcgcc
      181 aacatcgagt tcacgtatca ggtgaagtcc aacaccgccc tgacgttggg cgcctaccgc
      241 aacgtgacca aggacaagat cgcgcccgtc aacgacacca ccgccaacgg cggcaacgcc
      301 cgcgtttctt acagtttctg atgggatagt tcacccccgt tgttcccaag gggggatttt
      361 tttgcgatta aaagtaaaat aaacttgaca aattgtataa taagatatac tatgcggaaa
      421 aaggaaagga ggtgttatgg ccaaaacacc agcacatttg gctatcaaaa accggctaaa
      481 cgttttgaga gccgaacgcc gaataacgca ggaacagcta gccaaagaag tcggcgtgac
      541 acgagcgacg atcattgcga ttgaagggga agattataat ccgtcattgg aactggcgtt
      601 tcggctggcc cggtatttcg atacggatgt tcagagttta tttttcatta aggaggggac
      661 aaaatgaaca aattcgacag gttttttttg aaaacttatt tgtacgggtt tttcttcgtg
      721 atcgggctgg ggattgtttt taattattgt ggtctggatg atatgcggaa cgccgtttca
      781 cctttacgcc aggtgaacaa cgtcggtggg tttgtgttcg cgtcctggat gcttatgacg
      841 atgtatctga gcctcaaatt gatcttttcc gggtcctttc gcgacgcggt gctgtccagg
      901 ataacgtttt tgaaagaacg cgacgagcgg gaaaaactgt tttccggaca ggcggcaaaa
      961 acgacattca tgctgacgct ggcggtcttg tttttcctgc tgagcctttc gtgttttgaa
     1021 gtttcggtct atcgggttcc gccggaaaaa gcgattaacg gaaaaacggg ggtgttgacg
     1081 ttgggcgtcc gtttggaatt attgaaggac aggtccgcac cggcgccgga gacattccgg
     1141 cgggacatat tttcacatac ggatttgccg atttccagca cggctctgat cttgttgctg
     1201 atcgggtggc aaattgcttc ttacaatttg tgcatgcggc ggtttggaat ccgatcgtag
     1261 gcataaaagg acccggcgcc ccgcaatgcg tctttcgaga cggcggcacc tgtgacaccg
     1321 gaacgtctta taaggaggcc gacgtggcgg tctattgact gtcaagggat tgcggcgctc
     1381 gccaaatccg tccgcgtttg atgtttgcgg gggcgcttcg cgacccgccc cgcgagcgac
     1441 gttgtcgacg acggtgattt gagaagaaaa aataaaaacc ccgctttgcg gcggggttac
     1501 ttttttcagc gaataggccg tgagtcaaaa cagcacgaac gtgcttattt ggaggcgagg
     1561 aatttcttga tctcggcgtc gttgggggtg atcttgaggg ccagggccca gatttcccgc
     1621 gccttgtcct tctggttgag ggcgaagtag gcgctgcctt tgcgtttcag ggccaagacg
     1681 ttgtccggtt ccagttccaa aacctcgttg caggccaaga ccgccaggtc gtagcggccc
     1741 tcgtagatgt tgttgagggc gatgaaaagc ttgttttcgg tgacggacat ccccggggcc
     1801 accgtttctt ttttggccac gtccgggtat tgggtctcca tgtaaccgac cagcctggtg
     1861 gtggaggggt cctgcggatt gatgtctcgc gcgtaacgca aggcgttcag ggccagtcga
     1921 gggtcctccg tgtcggccag gtaggcggac agtcctttcc ggatgaggcg ggcggctttg
     1981 cccgtgccgg tctcggccgg cttgacctcg acgatgttct ccagtttggt cttaaggagc
     2041 ttgtagcgtt ggttggaagg gtccagcccc gacaggttct cggtcttttt gagggccgcg
     2101 tcgtattggc cggcctccag gaccaggcgc agttcttttt cgttggggcc gagttgggcg
     2161 gagagctttt cgcgggcctg tttgatgagg cccggcgggg cggcctgttc cgggtcgatc
     2221 tccaggatct tttgccaata ggagatggcg cgggaatagt cggcgtcgtt gaagctttgc
     2281 agggcccgcg cgtagtgttc ctccagaagt ttctgtcttt cctcgtcggt gagcgaggcg
     2341 tagttcgagg cggccgtcag gggcgccgcg agaatcgcaa ggaatacggc gagagcgatc
     2401 tgttttttca tgttttttcc atggtcctca caatgttgat ttcaatgcgg cggttctttc
     2461 cccggccttc cagcgtggcg ttgtccgtca cggggcggtg ttccccgtag ccatacgccg
     2521 aaaagcggtc gggggggaga ccgtcgccga tgaagaacgc gatgaccgaa aaggcccggg
     2581 ccgccgacag ttcgaaattg tcccgatagg gccccgtgcg gatgggcacg ttgtcggtat
     2641 ggccttccac caccacggcg ttggggaggc ctttcaagag tccggcgatt tctcgcaggg
     2701 ctttttgggc ctcggatttc aacgtcgcct gccccgagtc gaacagcacc gggttggaca
     2761 gcgaaacccg gatgcgctgg gccgagagtt ccacctgcgc cagccccttc aacttgtcct
     2821 cattaaagta tttttccagc gcctgggcca cctccgtctc cttttcctgt ttgagcaggt
     2881 gggctttctg gtcttttccg gcgatctctt tctggagggc gttcagggcg cgttcgtagg
     2941 tggaggaggc ccccaggtag gcgtagccga agagcgccag gaacaggatg accagggtgg
     3001 acatcaggtc ggcgaagggg atcatccaga gtttttccga agactcctcc gttttttcct
     3061 cgagcgtgat gagctgggaa agttcctggt tctcggaccc ttccgcgaac tcgcccgcca
     3121 gggggtcggt gagttcctct tcgctttcga aatccagtcc gctcacggtc aggcggcctt
     3181 tcgaaggatg ttgatctcga tgcggcggtt gtggcgtcgg ccgtcggggg tgtcgttgtc
     3241 ggacacgggg cggtattcgc cgtagccggc cagaatgaaa cgttccgagg ggatgccgca
     3301 ttcgcgcgtg aagaattcca ccacggagga ggcccgggcc accgagagct cccagttgga
     3361 ggcgaactgt ttctgccgca cgggctggtt gtcggtgtgg ccctccacca ggatcttgtt
     3421 gggcagggag gccaacaggg gggccaggtc gcggatttcc cgtcgggcgc cgtcgctcaa
     3481 gacggcttct ccggagagga acaggacggg gttggtgagg gtgatgcgga tgcgttcctc
     3541 ggaggtctcc acctgggcga attttttctt ctgcaatacc tgggtgacgt cctcctcctg
     3601 gactttctgg atgatctgtt ccgccttgga ttcgacggcg ctctggggtt tttcgcggaa
     3661 ttggttggcc atgtcgttca tcatttgccg gcgggtttcc tcgggctggc
//
LOCUS       DAOTFD010000245         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_18733_length_3689_cov_3.963126, whole genome shotgun sequence.
ACCESSION   DAOTFD010000245 DAOTFD010000000
VERSION     DAOTFD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18733_length_3689_cov_3.963126"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="P5079_10580"
     CDS             complement(<1..759)
                     /locus_tag="P5079_10580"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY30466.1"
                     /translation="MNKRPTSPSDVTLTVSQINQKIGETISAGFPDPFWVTGEIQGYD
                     RDAAKAPTRRWGQVYFELVEKEKGSDSAKASLKAILWGDAQATIVQRLRSTSDKLTLR
                     DGLQVKFLCAVDFYWPRASLQMKILDVDPEFTLGDLERARQELLKNLKEKGLFDRNRS
                     VLFPPLPGNIGLITSEGSAAYHDFTDELKRSGYAFRVRLWDARMQGEETEASVCRGIA
                     LLEGDPEVDVIVLVRGGGSRSDLIWFDKEKIAYAV"
     gene            complement(791..3235)
                     /locus_tag="P5079_10585"
     CDS             complement(791..3235)
                     /locus_tag="P5079_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRY30467.1"
                     /translation="MMTMEERHACSGMMDSRFALAGSEPQYAPDRTFDTEHIRLEVEL
                     DFKQKALRGRCVTTLRAIVAGASTMVFDAVGFKLDFVRSAGRALRHEYDGRRLTAFLP
                     APVRAGTTIQIEIGYRLRRPPLGLYFVGPDKKYPHKPVQAWTQGEDEYARYWFPCHDA
                     PHERTTTEVVARVPRGFTAVSNGRLVRRTHGSRTSVFHFRQDIPHATYLVSLAVGRFT
                     ELRDSWRGRPVLYYCPPGREEDAKRAFGKTPKMMEFFSKKIGVPYPYPKYSQVAAVDF
                     IYGGMENTSATTQTALTLHDDRAHLDFSSDSLVAHELAHQWFGDLLTCKSWPHAWLNE
                     SFATYFEALFQEYDKGKDEFCNELHQNAEAYFDEDKDHYRRPIVTKVYKRPSDLFDRH
                     LYEKGSLVLHMLRHLLGEDGFWKSIHAYVVEHRGKAVETRDFIDAIEKATGRNVEKFF
                     DQWIYKAGHPEYKVRFWWVQRTKQVHLRVVQMQRAGEETGLFSLPVTFSFWTSSGERR
                     FTEVVERKSHLFKFKLPSAPQLVLFDPDHVILKRVDFPKPEAMWITQLRRDPHPLGRL
                     AAAKVLGRLATPESVKALAEALLKETFWLVQGEIAWSLGSIRTPSAVAALWRGFEAVR
                     HPKARRVLIAALGEVRTGEVLERLRAAYKKEKSYFAEAQALKSLARCGHPSVKEIIRE
                     QMKEESWNEVLRCGALEAMTQLSAPEVEAFLRKYTVYGHPNVLRQTAVRCLAQLPAPG
                     AGLQKALLALTRDPYLLVRLAAVRALRVVGDERAVPALKKLTRGDLDGRLMRAAEEAV
                     QQLQKGIEPSEKKADH"
     gene            complement(3232..3492)
                     /locus_tag="P5079_10590"
     CDS             complement(3232..3492)
                     /locus_tag="P5079_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30468.1"
                     /translation="MTVTVLVASLAAFFCLAVIFRRTIGGSEPPRPSRGEPALHSDLA
                     EAIVRFDKWRSEGRISREDYERFMKLCQEDAAVKEKSEGFRS"
     gene            complement(3509..>3689)
                     /locus_tag="P5079_10595"
     CDS             complement(3509..>3689)
                     /locus_tag="P5079_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30469.1"
                     /translation="ALRPTVDEFFKGVMVMDPNEEVRLNRLNLLIRLTALFRRLADLS
                     RLQDVPSAQAVSQKS"
BASE COUNT          733 a         1171 c         1064 g          721 t
ORIGIN      
        1 cacggcgtac gcgatctttt ctttgtcgaa ccagatcagg tcggaacgcg aaccgccgcc
       61 gcgcaccaac acgatgacat ccacctccgg atcgccttcc agcaacgcga tcccccggca
      121 gacgctcgcc tcggtctcct cgccctgcat gcgggcatcc cacaggcgga cacgaaacgc
      181 atagcccgat ctcttcagtt cgtccgtaaa atcgtgatag gccgcgctgc cttccgaagt
      241 gatcaatccg atgttgccgg gaagcggcgg gaaaaggacg gaacggttcc tgtcgaaaag
      301 tcccttctcc ttcagatttt tcaggagttc ttgacgggcg cgttccaaat cccccagcgt
      361 gaactccgga tccacatcca aaatcttcat ctgcagactg gcgcgcggcc aataaaaatc
      421 caccgcacag agaaatttca cctgaagacc gtcccgcagc gtcaatttat ccgacgtgct
      481 ccgcaggcgc tggacgatgg tggcctgcgc gtctccccag aggatggctt ttaggctcgc
      541 cttggcggag tcggacccct tttctttttc caccagttca aaatagacct gtccccagcg
      601 ccgggtcggg gcctttgcgg catcgcggtc gtatccctgg atctcaccgg tcacccagaa
      661 cgggtccgga aatccggcag agatcgtttc tcctattttt tgattgatct gactgacggt
      721 gagcgtcaca tcggacgggg atgtcgggcg tttgttcatc gtctctctcg aacgtccggg
      781 agaccgtttt tcaatggtcg gcttttttct ccgatggttc gatccccttt tggagctgct
      841 gcacggcctc ctcggcggcg cgcatcaatc gcccgtcgag gtcgccgcgc gtcaacttct
      901 tcagggccgg cacggcccgc tcatctccca cgacacggag cgcgcgaacc gccgccaacc
      961 ggaccagcag ataggggtcc cgcgtcaacg caagcagggc cttttgcaac ccagcccccg
     1021 gcgccggaag ctgggcgagg caacgcacgg cggtctgccg caaaacattc ggatgcccgt
     1081 agacggtgta tttccgaagg aacgcctcca cctccggcgc agacagctgg gtcatcgcct
     1141 ccaaggcgcc gcaccgcagg acttcgttcc acgactcctc cttcatctgt tcccggataa
     1201 tctccttgac cgaaggatga ccgcaacgcg ccaagctttt cagcgcctgc gcctcggcaa
     1261 agtagctctt ctctttttta taggccgcac ggagccgctc cagcacctcc ccggtgcgga
     1321 cttccccgag ggcggcgatg aggacgcgcc gcgccttcgg atgccgcacg gcctcgaaac
     1381 cccgccacag ggcggcgaca gcggacgggg tgcggatgct ccccaaagac caggcgattt
     1441 cgccctgcac aagccaaaac gtttctttca acaaggcctc cgccagcgct ttgacggatt
     1501 ccggcgtcgc cagacgaccc aacacctttg ccgcggccaa acggccgagg ggatgtgggt
     1561 cccgccgcaa ttgcgtgatc cacatcgcct ccggtttcgg aaaatccacc ctcttcagga
     1621 tgacgtggtc cgggtcgaac aggacgagtt gaggcgccga gggcagttta aacttgaaca
     1681 ggtgcgattt cctctcgacg acctcggtaa aacgcctctc cccggaggag gtccagaacg
     1741 aaaacgtcac gggcagggaa aacagccccg tttcttcccc cgccctctgc atctgaacga
     1801 cgcgcagatg gacctgcttc gtcctctgaa cccaccagaa acgcaccttg tattccggat
     1861 gaccggcttt gtatatccac tggtcgaaga atttttccac gtttcgtccg gtagccttct
     1921 cgatggcgtc aatgaaatcc cgcgtctcca cggctttgcc ccgatgttcg accacgtagg
     1981 catggatgga cttccaaaac ccgtcctccc ccaggagatg gcggagcatg tgaagcacga
     2041 gggagccctt ttcgtagaga tgccgatcga acagatccga cggtcgcttg tagaccttgg
     2101 tcacaatggg acgacggtag tggtccttgt cctcgtcgaa ataggcctca gcgttctgat
     2161 ggagttcgtt gcagaactcg tctttcccct tgtcgtattc ctgaaagagc gcctcaaagt
     2221 aggtggcgaa actctcgttg agccaggcgt ggggccagct tttgcacgtc aacagatcgc
     2281 cgaaccactg atgggccagt tcatgcgcca ccagggaatc gctggaaaaa tccagatggg
     2341 cccggtcgtc gtgcagggtc agggcggtct gggtggtggc gctggtgttc tccatgcccc
     2401 cgtagataaa atccaccgcc gccacttggg aatatttggg gtagggatac ggaacgccta
     2461 tttttttgga aaagaattcc atcatcttcg gcgttttccc gaaagcccgt ttcgcgtcct
     2521 cttcccggcc gggggggcag taataaagca cgggccgacc gcgccagctg tcgcgcagtt
     2581 ccgtaaaacg accgaccgcc aaggaaacca gataggtggc gtgcgggata tcttgtcgaa
     2641 aatggaacac cgacgtccgg ctcccgtgcg tccggcggac cagccggccg ttggacaccg
     2701 ccgtgaaccc ccggggaacg cgggccacga cctccgtggt ggtgcgttca tgcggggcgt
     2761 cgtggcaggg gaaccagtag cgggcgtatt catcctctcc ctgggtccag gcctgtaccg
     2821 gcttgtgggg atatttcttg tccggcccga cgaaatagag ccccaggggc ggccggcgga
     2881 ggcggtatcc gatctcgatc tggatcgtcg tccccgcccg gacgggggca ggaaggaacg
     2941 cggtcaaacg ccggccgtcg tattcatggc gaagggcgcg acccgccgaa cggacaaaat
     3001 ccagtttaaa accgacggcg tcaaaaacca tggtcgaggc cccggcgacg atggcgcgca
     3061 acgtcgtgac gcagcggccc cgcaaagctt tctgtttgaa atcgagttcc acctcaaggc
     3121 ggatgtgttc cgtgtcgaac gtgcggtccg gggcgtactg cggttcgctg cccgccaggg
     3181 caaaacggga atccatcatc cccgaacagg cgtgccgttc ttccatcgtc atcatgagcg
     3241 aaaaccctcc gatttctctt ttaccgccgc atcttcctga cagagtttca tgaaccgttc
     3301 gtaatcttcc cgactgatac gcccctcgga acgccactta tcgaaacgga caatggcctc
     3361 cgccagatcg ctgtggaggg cgggttcccc tctggacggt cgggggggtt ccgaaccgcc
     3421 gatggtcctt cggaaaatga cggcgaggca gaaaaaagcg gccagcgaag cgacgaggac
     3481 ggtgacggtc atcggaataa cgccgtctct aacttttctg cgaaaccgcc tgggcggacg
     3541 gcacatcctg caagcgggac aaatcggcga ggcggcggaa aagggcggtc aggcggatca
     3601 ggagattcag ccggttgagc cgcacctcct cgttcggatc catcaccatg acccctttaa
     3661 agaactcatc caccgtcggc cgaagggcc
//
LOCUS       DAOTFD010000246         3668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_18875_length_3668_cov_3.939662, whole genome shotgun sequence.
ACCESSION   DAOTFD010000246 DAOTFD010000000
VERSION     DAOTFD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18875_length_3668_cov_3.939662"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2870
                     /locus_tag="P5079_10600"
     CDS             <1..2870
                     /locus_tag="P5079_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30470.1"
                     /translation="GKETFNAVGDLTLARPAEESPRPDQSPEQPQLEPAQPKPAKDVM
                     DAREKEMRANLAAAKTDADRTVILEQLRTLFQRRLDRNVKMLSAAQVTQDDKMRQQVQ
                     ARIDRYQAKLRALEAEMPSVSRPAEERVDKPAERPRRLQPDIAQEDLAKPEKKKRVRT
                     VARSSEKGFVSTGLLTAMGGVALLVLALPALKAAGVALSAVLLAKVGLVVVGAAGGAY
                     LLWKAVPPLADWAKRVAAGVRIRVSDDKMRQAVDAELIRPNAATQRSLAARGTESALF
                     ASISWQGRVSVDVLPGLRLTRDELMSRMKDAVTGADKSVIYSEDAALLPKKLLQPHKV
                     VGHTHLGNTTDLPVSRLAEFLRPTVDDLLSAWKHGGAHFVVNPADTRVRVYYKVSDNG
                     AVKIWEYTGDTPETVSVRVTETSVSAILLEDMTNPAKFVTVTDAAGVPATVPVWFFAL
                     AAGLQVRGLSDIYPDLPTEEKARRLMQIHDQIWKSMDRSRVQGPDVKPVVVNAMVAFF
                     LTNLSKDAEALRPLLEKSAERMLDPFLNELYQEAYKGREMPPFDMDSNLSWRTAQLNA
                     RRAELADAALKVSVRELPAAANDLFGRYLAGARSAGMEPMAEPWSIEVVMESEPAAEI
                     DNLTGNSWMDPPETAEGKRVYRIHIIPTADPVQNLARLAHEAAHAALSHTRARVEPAA
                     HDEFTEILVNKLMERSLEGTEWEAVSVEVMRLTVDHIRRNNLTSFWRGQQLLDALTAH
                     VERSGAYTEAQRLALLQRIFGIRDMTVDPLAFIKEMIVQLLEPVTLPDTTRINRPSAI
                     ALDEDTLFGSDSPAAILALRKMLENNPHAYLVVYPSSGAKKNAQSTLLPRVLAAMQNV
                     SSDDISAIQGRIIAVDWFHVQDMNKAGKVSLSRLMGLLPQLIMGLEGVRVVTSNEDLY
                     TGRELLGKLLISLAKGETLLQGLGTAISDMVAEKELLDKQA"
     gene            complement(2969..3469)
                     /locus_tag="P5079_10605"
     CDS             complement(2969..3469)
                     /locus_tag="P5079_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRY30471.1"
                     /translation="MSTTDNLKAAFAGESQANRKYLAFAKKAESDGFPQIAKLFRAVA
                     EAETVHAHAHLRVLGGVKKTAENIQSAMDGEAYEFKTMYPDFLAESEKEGHRAASLTF
                     KYALAVEKIHHDLYSKALAASQTGKDLETAPIYVCEVCGHTHIGLPEDKCPICGAGKD
                     RFKEIK"
BASE COUNT          696 a         1068 c         1206 g          698 t
ORIGIN      
        1 aagggaaaga aactttcaat gcggtgggtg atttgacttt ggcgcgtccg gcggaggagt
       61 cgcctcggcc ggatcagtcg ccggaacagc cccaactcga gccggcgcaa ccgaaacccg
      121 cgaaagacgt gatggacgcg agggaaaaag aaatgcgggc gaatctggcg gcggcaaaga
      181 ccgacgcgga ccggaccgtg atcctggaac agttgcggac cctcttccag cgtcggttgg
      241 accggaacgt gaagatgctg tccgccgccc aagtgacgca agacgacaag atgcggcagc
      301 aggttcaggc gcggatcgac cggtatcagg cgaaactgcg ggctttggag gcggagatgc
      361 cgtcggtgag ccggccggcg gaagagaggg tggataagcc ggcggaacgc ccgagaagac
      421 tgcaacccga tatcgcccag gaagacctgg ccaaaccgga gaagaagaaa cgggtgcgga
      481 cggtcgcgcg ctcttccgaa aaaggttttg tctccaccgg gctgctgacg gcgatgggcg
      541 gcgtggccct gctggttctg gctttgccgg ccctgaaagc ggcgggtgtg gcgttgagcg
      601 ccgtgcttct ggcgaaagtc gggctggtgg tcgtgggcgc ggccggcggg gcgtatctgc
      661 tttggaaggc cgtgccgccc ttggcggact gggcgaaacg ggttgccgcc ggtgtccgga
      721 tccgcgtttc cgacgataaa atgcgtcagg cggtggatgc ggaactcatc cggcccaacg
      781 cggcgaccca gcgctctctg gccgcccgtg gcacggaatc ggccctcttt gcctccattt
      841 cctggcaggg acgagtgagc gtggacgtgc tgcccggcct gcggctgacc cgggacgaac
      901 tgatgtcccg catgaaagac gccgtcaccg gcgccgacaa gagcgtgatc tattccgagg
      961 acgcggccct gctgccgaag aagcttctcc agccgcacaa ggtggtgggc cacacccatc
     1021 tgggcaacac gaccgacctg ccggtgagca gactggccga gttcctgcgg cccacggtgg
     1081 acgacctgct ctccgcctgg aaacacggcg gcgcgcattt cgtggtgaac cccgccgata
     1141 cccgcgtgcg cgtgtattac aaggtgtcgg acaacggggc cgtgaagatc tgggaataca
     1201 ccggcgatac gccggaaacc gtttccgtcc gtgtgacgga aacctccgtt tcggccatcc
     1261 tgctggagga catgaccaac ccggccaagt tcgttaccgt gaccgacgcc gccggggtcc
     1321 ccgccacggt gccggtgtgg ttcttcgccc tggccgccgg cctgcaggtg cgcggcctca
     1381 gcgatatcta tcccgatctt ccgacggaag agaaggcccg gcggctgatg cagatccacg
     1441 accaaatatg gaagagcatg gaccgctccc gggtccaggg gccggacgtt aagcccgtgg
     1501 tggtgaacgc catggtggcc ttcttcctca ccaacctttc caaggacgcc gaggccctgc
     1561 ggcctctgtt ggagaagagc gcggagagga tgctcgatcc tttcctgaac gaactctatc
     1621 aggaggccta caaaggccgt gagatgccgc cgttcgacat ggattccaac ctttcctggc
     1681 ggacggcgca gttgaacgcc cggcgcgcgg aactggccga cgcggctctc aaggtctccg
     1741 tgcgggaact gccggccgcg gccaacgacc tcttcggccg ttatctggcc ggcgcccggt
     1801 cggcggggat ggagccgatg gccgaaccgt ggtccatcga ggtggtgatg gagagcgagc
     1861 cggccgccga gatcgacaac ctcacgggga attcgtggat ggatcccccg gagacggcgg
     1921 aggggaaacg ggtctaccgg atccacatca tcccgacggc agaccccgtc cagaacctgg
     1981 cgcggttggc gcacgaggcg gcgcacgccg ccttgtctca cacccgcgcc cgcgtggaac
     2041 cggccgcgca cgacgagttc accgaaattt tggtcaacaa gctgatggaa cgttctctgg
     2101 aggggacgga gtgggaggcg gtttccgtgg aggtgatgcg gctcacggtg gaccatatcc
     2161 gccggaacaa cctgacctct ttctggcgcg gccagcagtt gctggatgcc ttgacggcgc
     2221 atgtggaacg ctccggcgcc tacacggagg cgcagcgtct cgccctcttg cagaggatct
     2281 tcggcatccg cgacatgacc gtggatcccc tggcgttcat caaggagatg atcgtccagc
     2341 ttctggaacc cgtcaccctg ccggacacga cccgcatcaa ccggccgtcg gccatcgcgt
     2401 tggatgagga cacccttttc ggcagcgatt ccccggcggc catcctggcc ttgcggaaga
     2461 tgttggaaaa caacccgcac gcctacctgg tggtctatcc cagcagcggc gccaaaaaga
     2521 acgctcaatc gaccctgctg ccgcgggttc tggcggccat gcagaacgtt tcctccgacg
     2581 atatttcggc catccagggg cgcatcatcg ccgtggactg gttccatgtt caggacatga
     2641 acaaggccgg aaaagtgagc ctttcccggc tgatgggcct gctgcctcaa ctgatcatgg
     2701 ggttggaagg cgtgcgcgtc gtcacgtcga acgaagattt gtatacgggt cgggaactcc
     2761 tcggaaaact gctgatctct ctggccaaag gggaaacgtt gctgcagggg ctggggaccg
     2821 ccatcagcga catggtggcg gagaaagaac tgctcgacaa acaggcctga cttttggcgc
     2881 ggcgcaagga caaggaattg aaataaaaaa tcctccggtc gacggaggat tttttattgt
     2941 cagtggaatg tagggtggga aaagccgctt attttatttc cttgaatctg tccttgccgg
     3001 cgccgcagat gggacatttg tcttcgggaa ggccgatgtg ggtgtgcccg cagacctcgc
     3061 agacatagat gggggctgtt tccaggtcct tcccggtctg cgaagccgcc agcgccttcg
     3121 aatagaggtc gtggtggatt ttttcaacgg ccaaggcgta tttgaaggtg aggctcgccg
     3181 cccgatgacc ctctttttcc gattccgcca gaaaatccgg atacatggtt ttgaactcgt
     3241 aggcttcgcc gtccatggcg gactggatat tctccgcggt ttttttgacg ccgccgagaa
     3301 cgcgcaggtg ggcgtgggcg tgcacggtct ccgcctcggc cactgcgcgg aacagcttgg
     3361 cgatctgggg gaaaccgtcg ctttcggctt ttttggcgaa cgccaggtat tttcggttgg
     3421 cctggctttc gccggcgaag gcggctttta aattatccgt ggttgacatg gggcctcctg
     3481 agtcggtgca agataagaat tcgtttcaaa gcgacgttca agcggctgtt tttatatcct
     3541 ttttgttccg aaaataccag tgaaaatttt cttacggaaa agtcctgcgg gcttgaataa
     3601 tttttttccg gcggggctgt tttttcaact cccacgggtc cttggggtgg ttagaccggc
     3661 gggcgaga
//
LOCUS       DAOTFD010000247         3642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_19090_length_3642_cov_3.251742, whole genome shotgun sequence.
ACCESSION   DAOTFD010000247 DAOTFD010000000
VERSION     DAOTFD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_3642_cov_3.251742"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="P5079_10610"
     CDS             complement(<1..339)
                     /locus_tag="P5079_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30472.1"
                     /translation="MGDASKNVLVVDDEEMIRAVWGRVLSKCGFNVFSAASGREALRK
                     LQEHPVQIVFTDLKMPEMSGLDLLKHIKDTQYPSCVNIITGVATVEGAVECLHLGACD
                     YVCKPCNLEEI"
     gene            complement(373..1947)
                     /locus_tag="P5079_10615"
     CDS             complement(373..1947)
                     /locus_tag="P5079_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30473.1"
                     /translation="MTDPVKILVVDDEEGMRDMLSYELASKGYRITTAADGEEAIGKA
                     RNEKFHLVLSDLKMPKVDGIQTLRAVKALDPDVEVIISTGYGTIETAVEAMKYGAYDF
                     VQKPFNMDEVFLLIEKALEKKELRALAAIYKTSRSVFNTIRADVLFPRAVEAAREIFS
                     ADGVCLMLMDEAGALTTAAALGCDEAVLGLVRRTVQEKLSGGKPVPPDPLAVALSPDP
                     ASAAGPGIFCPLVVDGNLLGVLYAGQRKGEKPFLSDELQRCGIFCSQLAHAVYNVRLY
                     RALEQNIGRLEEANLRLKETQAKLFQTEKLAAVGQLAAGVAHELNNPLTGIMGFAQLL
                     LDDKTLTPSQREDLECIHKQSQRCRQIIQNLLQFSRREEPRKEKLAVVALLEATLDLV
                     KYDFSSSGIRIVKEFPEESPVLLGDKSQLQQVFLNLLTNARQAVGDRKDGRIEIVVGR
                     SPSGVSVVFRDNGVGISEENMSRMFDPFFTTKPVGQGTGLGLSISYGIVQQHGGALSV
                     RSELGVGSEFLVEFPV"
     gene            complement(1988..2461)
                     /locus_tag="P5079_10620"
     CDS             complement(1988..2461)
                     /locus_tag="P5079_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30474.1"
                     /translation="MMENDGQELSKAGRVVGRVADPLGRPPRFAGDSSPKGRVLVVDD
                     EPVVRKVLERFLRSKGLSVSVAEGGVEALSLVKKERPHIMLLDLWMPKMNGIEVLKAV
                     REIDREIAVIISTANADVDIARKTMEMGACDYIIKPFDLPYLETALSARLLMLTA"
     gene            complement(2490..2900)
                     /locus_tag="P5079_10625"
     CDS             complement(2490..2900)
                     /locus_tag="P5079_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30475.1"
                     /translation="MSAKGSHSEKKQDGPGRRILVVDDEPGFREILRACVEGRRCEVE
                     TARDAAEAMALLEQRHYDLVVTDIVMPGLSGLNLLEKVRTMCPGTAVIITTGLGTVET
                     AVYAMKRGAADFILKPFRLDVIESRIREVLSLAV"
     gene            complement(2910..>3642)
                     /locus_tag="P5079_10630"
     CDS             complement(2910..>3642)
                     /locus_tag="P5079_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY30476.1"
                     /translation="AAVGQLAAGVAHEINNPLGVILGFAQGVVRRLAAGDKLELPLRS
                     IEREALRCKNLVQDLLTFSRVHEPDRGPIELNQAVEGALHLIEARARISRVEVEKQLT
                     EKIPAILGNKVQIQQIIINLANNAIDAMPDGGKLTIATELAEEGIHSWVCLKVGDTGN
                     GVPSELRQHIFEPFFTTKPVGQGTGLGLSLVHEIVRRHSGSIDLQSRPGETTFTIKFP
                     VRTGREPERVFAATLEKSKDGSVRG"
BASE COUNT          701 a         1263 c          939 g          739 t
ORIGIN      
        1 gatctcctcc aggttgcacg gcttgcacac ataatcgcag gcccccaaat gcaggcactc
       61 caccgcccct tcgaccgtcg ccacgcccgt gatgatgttg acgcaggagg ggtattgggt
      121 gtctttgatg tgcttcaaaa gatccagccc gctcatttca ggcatcttta aatcggtgaa
      181 gacgatttga accgggtgtt cctgaagttt ccgcaaagcc tcccggccgc tggcggccga
      241 aaaaacgttg aaaccgcatt tgctcaggac ccggccccaa acggcgcgga tcatctcctc
      301 gtcgtccacc accaagacat ttttagaagc atctcccatg ttcgcgcccc ccccggtttt
      361 ttcagaccgg cctcatacag gaaactccac caggaactcg ctccccaccc ccagctcact
      421 ccggacgctc aacgccccgc cgtgctgctg cacgatgccg taggagatgg agagacccaa
      481 cccggtcccc tgtccaaccg gcttggtcgt aaaaaacgga tcgaacatac gggacatgtt
      541 ttcctcgctg attcccacgc cgttgtcccg aaagacgacg gaaacgccgg agggcgagcg
      601 ccccacaacg atctcgatac gaccgtcttt ccggtccccc acggcctggc gggcgttggt
      661 cagcaggttc aaaaaaacct gctgcagctg gctcttgtct cccagaagca cgggagactc
      721 ctccggaaat tctttgacga tccggatccc cgacgacgag aagtcgtatt tcaccaggtc
      781 caacgtggcc tccaggagcg cgacgacggc gagtttttcc ttccggggct cctcgcggcg
      841 gctgaattga aggaggttct gaatgatctg gcggcagcgc tgactctgtt tgtgaatgca
      901 ttccagatcc tcgcgttgag agggcgtcag ggtcttgtca tccagaagca actgagcgaa
      961 acccatgatg cccgtcaacg gattgttcaa ctcatgggcg accccggcgg ccagctggcc
     1021 gacggccgcc aacttctcgg tctgaaacaa cttcgcctgc gtttctttca accgcagatt
     1081 cgcctcttcc aggcggccga tgttttgctc cagcgcccgg tagagccgga cgttgtatac
     1141 ggcatgggcc aactgggaac aaaagatccc gcatcgctga agttcatccg acaggaacgg
     1201 tttttctccc ttccgctgcc ccgcgtagag gacccccaga aggttcccgt ccaccaccag
     1261 gggacaaaag atgccgggcc ccgccgccga ggcggggtcc ggtgaaagag cgaccgccag
     1321 ggggtccggc gggacgggtt ttccgccgga cagtttctcc tgaaccgtcc ggcggacgag
     1381 ccctaagacc gcctcgtcgc aacccagggc ggcggcggtc gtcagggctc ccgcctcgtc
     1441 catgagcatc agacagacgc cgtcggcgga aaagatctcc cgcgccgcct ccaccgcgcg
     1501 aggaaaaaga acgtccgccc ggatggtatt gaacaccgaa cggctcgtct tatagatcgc
     1561 cgccagcgcc cgcaactctt ttttctccag ggccttctcg ataagcaaaa aaacctcgtc
     1621 catgttgaac ggtttctgca caaaatcata ggcgccgtat ttcatggcct ccacggcggt
     1681 ctcgatggtg ccgtaccccg tgctgatgat cacctccacg tccgggtcca acgccttgac
     1741 ggcccggagc gtctggatcc cgtcgacttt cggcatcttc aggtcgctca agaccagatg
     1801 gaacttttcg ttccgcgctt ttccgatggc ctcttcccca tccgcggcgg tggttatccg
     1861 atagcctttg gaagccagct catacgaaag catgtcgcgc atcccctcct cgtcatccac
     1921 caccagtatc ttcaccggat ccgtcatgac cgccgcctcc ttgattttca gatcgtccca
     1981 gcccgtctca ggccgtcaac atgagcaacc gggcgctgag ggccgtttcc aggtagggca
     2041 ggtcgaacgg tttgatgatg tagtcgcagg cccccatctc catggttttc cgcgcgatgt
     2101 ccacatccgc gttggccgtg ctgatgatga cggcgatctc ccggtcgatc tcgcgcaccg
     2161 ctttcaggac ctcgatgccg ttcatcttcg gcatccacag atcgaggagc atgatgtgcg
     2221 gccgttcctt cttcaccagc gacagcgcct ccaccccccc ctccgccacc gacaccgaca
     2281 accccttgct tctcaaaaac cgctccaata ccttccgaac caccggttcg tcatccacca
     2341 ccaacacccg ccctttcggc gacgaatctc cggcgaaacg aggcggcctt cccagggggt
     2401 ccgccacccg ccccaccacg cggcccgctt tcgacagttc ctgtccgtcg ttctccatca
     2461 tgttttcctc ccgtctcctg cgatttcggt caaacggcca atgacaagac ttcccgaatc
     2521 cgcgactcga tgacatccag gcggaacggc ttcaggataa aatccgcggc gccccgtttc
     2581 atggcataca cggcggtctc aacggtgcct aatcccgtgg tgatgatcac cgccgtgccc
     2641 ggacacatcg tccggacctt ctccagcaaa ttcagcccgc tcaatccggg catcacgatg
     2701 tccgtgacga ccaggtcata atgcctttgc tccagaagag ccatcgcctc cgctgcgtcc
     2761 cgggcggtct ccacctcaca tctcctcccc tcgacgcaag cgcggagaat ctcacggaac
     2821 cccggctcgt cgtccaccac caagatccgc cgccccggac cgtcctgctt tttttccgaa
     2881 tgagagcctt tcgcggacat atttttctcc tatccccgga ccgacccgtc cttcgacttt
     2941 tccagcgtcg ccgcaaaaac gcgctccggc tcccgccccg tgcgcacggg gaattttatc
     3001 gtgaaggtgg tttcaccggg acggctttga agatcgatgc tcccggaatg ccgccgaacg
     3061 atctcatgca ccaggctcaa ccccaaaccg gtcccctgtc ccaccggctt ggtggtgaaa
     3121 aacggctcaa aaatatgctg acgaagctcc gaagggacgc cgttccccgt atcgcccact
     3181 ttcaggcaga cccaggaatg gatcccctct tccgcaagct ccgtggcgat ggtcaatttt
     3241 ccaccgtccg gcatggcatc gatggcgttg ttggccaagt tgatgatgat ctgctgtatc
     3301 tgtactttat tccccaaaat agccggaatt ttctccgtca attgtttctc cacctccacc
     3361 cgactgatcc gggcgcgagc ctctatcaaa tgcagagcgc cctccacggc ctggttcagc
     3421 tcgatcggtc ccctgtccgg ctcgtggacc cgggaaaatg tcagcagatc ctgcaccagg
     3481 tttttgcagc gaagggcctc cctctcgatg gaccgcaggg gcagctccaa tttatcgccg
     3541 gccgcgaggc gccgcacaac cccctgggcg aacccgagga tgacacccag cggattgttg
     3601 atttcgtgcg ccacgccggc ggccagctgg cccaccgccg cc
//
LOCUS       DAOTFD010000248         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_19214_length_3627_cov_5.386058, whole genome shotgun sequence.
ACCESSION   DAOTFD010000248 DAOTFD010000000
VERSION     DAOTFD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19214_length_3627_cov_5.386058"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="P5079_10635"
     CDS             <1..240
                     /locus_tag="P5079_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30477.1"
                     /translation="PLFAAGPELMCQSAFDYFQVDDRPKEEVYRAIVKRILASKAVND
                     AVKSELSIKTLPRLLKQGLKAGLEFRKIRKEALKS"
     gene            254..556
                     /locus_tag="P5079_10640"
     CDS             254..556
                     /locus_tag="P5079_10640"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY30478.1"
                     /translation="MANELPKVNVDEKLGYLTYKADHQSHITIPDGKICVEQCHDRPC
                     LTGCPAQVYVWEETQKKVLVSFENCIECGACRMLCPFDNIKCHWPRGGFGVQYRYG"
     gene            775..2643
                     /locus_tag="P5079_10645"
     CDS             775..2643
                     /locus_tag="P5079_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRY30479.1"
                     /translation="MKRRFSSSYKTFLKALSALLLGMFLLPSAASALDLGIDGLRVDW
                     SPSHTGGTTLTWDSVDPSTTDAIVTYYVLRLAGYHPSTTTVVLNGTTVAVTNVTTYKF
                     GDGGVYTYIVRAFDSLATPSFVDDSPVQYSHLIHYDLSPVSQNITRYDAGNGQDGNGQ
                     QWNFEYKLQDDAYVTIAVYPETCTFGKDAAGFYTTPSSGPIKVILDNTPRSNQMVDDS
                     FVNKDFWDLRNSSGAVVGKGLYQVLFTVKSKYDATTVIDTALKTLPVDTIRIADISAS
                     ALTDASGYSAISFNVNADCEVKVIVCKPGTKFAVATASGTLGYLTASNYAYVVGDNLP
                     LNPTTLVVDGTRLLKVLRYYVDSGNQTFSWNGVDEEGNTLASSIYPYTLSAMDAFNNH
                     AVLVAGDNYSQFGTITINRVATQPRAISLAAVYPAPNETVTSRINSVYAVLDPKRTDV
                     TVDGSISTLYLTGPNNTTVIGQKRWDDAQKRMVYYLTQPLTQDGQYTMNVYARGKTIW
                     GDDVREDYTSSFVLQIPHALQDSYVSPNPLKGASSANFGITLNSAATVRLRIFNLLGE
                     EVYDRTESFAAGPQIIAWNLRNDSGQRVASGVYIYRLEVDGPGGSEEVTKKLVVIQ"
     gene            2655..3626
                     /locus_tag="P5079_10650"
     CDS             2655..3626
                     /locus_tag="P5079_10650"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30480.1"
                     /translation="MKRAAVLCLSLGLTGAAFGGSKDAGTASAVFLRDGQGARALGMG
                     GAYTAVGRGVSAMFWNPGGLSRTSQMGVMTSYNTLIEDVKTQSVSFLCPLSKGEKGAI
                     GVSAVMMNVGDIEKRDGTGTLLGEGKVKGGAYGLGYGFHLGRWGFGAQVKSVNEDLIA
                     EKGKATAVDAGVLGQWGSVSVGLSAQNFGGKYKVGGLENDLPGVLRGGVAYHPVETEN
                     QWLERMTVSADVEKPVDQDWKVRGGGEYVLSRMLSVRAGYEQMRDLDSSGISFGLGLW
                     TDFKKMEKEEGDYRRNTESRVVMEVDYAYLMRDVFDDLHRVTLSLHF"
BASE COUNT          736 a         1036 c         1180 g          675 t
ORIGIN      
        1 ccgcttttcg ccgccggacc cgagttgatg tgtcagtcgg ccttcgatta tttccaggtg
       61 gacgaccggc ccaaggagga ggtctaccgg gccatcgtga agcggatttt ggccagcaag
      121 gccgtgaacg acgccgtcaa gtcggagctg agcatcaaaa ccctgccgcg cctcctcaag
      181 caggggctca aggccggact ggagttccgg aaaatacgca aagaagcgct gaagtcttaa
      241 cgaggacgaa aatatggcca acgaacttcc gaaagtcaac gtcgacgaga aactgggata
      301 cctcacctac aaggcggacc accagtccca catcaccatc cccgacggca agatctgcgt
      361 cgagcaatgc cacgaccggc cctgcctcac cggctgcccc gcccaggtgt acgtgtggga
      421 ggagacccag aagaaggtgc tcgtttcgtt cgaaaactgc atcgagtgcg gtgcctgccg
      481 catgctctgt ccgttcgaca acatcaaatg ccactggccg cgcggcgggt tcggggtgca
      541 gtaccgttac ggctgaggag tcggtcgttc cccgcttcga taggtaaaca gcccggaaaa
      601 ggcaggttcc gggctgttta agtttgtccc cgggtcggcc gccggttggc tccggtcggg
      661 cggaattttg gggcagaaac tatttttttc gccaaattat gctatcatca tccgcgggaa
      721 atttttaacg ggcgaaaggg acgtttcgcc ccattctcgg agcgtgacga gctgttgaaa
      781 agacgttttt cctcttccta taagacattc ctgaaggcgc tctccgcgct ccttttgggg
      841 atgtttctcc ttccttccgc tgcttccgcg ctggatctcg gcatcgacgg gctcagggtg
      901 gactggtctc cctcccacac cggcggaacc acgctgacct gggacagcgt cgatcccagc
      961 accaccgacg ccatcgtcac ctactacgtg ttgcgcctgg cgggatatca cccgtccacc
     1021 accaccgtgg tgctgaacgg gacgaccgtg gcggttacca acgtcacgac ctataagttc
     1081 ggagacgggg gggtctacac gtacatcgtg cgcgcgttcg attccctggc gacgccgtcg
     1141 ttcgtggacg actctccggt gcagtacagc cacctcatcc attacgacct cagcccggtt
     1201 tcccagaaca tcacgcgtta cgacgccggc aacggccagg atgggaacgg gcagcagtgg
     1261 aatttcgagt ataaactgca ggacgacgcg tatgtgacca tcgccgttta tccggaaacc
     1321 tgcaccttcg gcaaagatgc cgccggtttt tataccacgc cgtccagcgg ccccatcaag
     1381 gttatcctgg acaacacgcc gcgttccaac cagatggtgg acgactcctt cgtcaataag
     1441 gatttctggg acctgaggaa cagttccggg gcggtggtgg ggaaagggct ttatcaggtg
     1501 ctgttcacgg tcaaatcgaa gtatgacgcc acgaccgtca tcgataccgc cctgaagacg
     1561 ttgcccgtgg ataccatccg catcgcggac atctccgcct cggcgctgac ggacgcctcc
     1621 ggctattccg ccatcagttt caacgtcaac gccgattgtg aagtgaaggt gatcgtctgc
     1681 aagccgggca ccaaattcgc ggtggccacc gcgtccggca cgctcggcta tctcaccgcc
     1741 tccaactacg cctacgtggt cggcgacaac ctcccgctca accccaccac tctggtggtg
     1801 gacggtaccc gtctcctgaa ggtcttgaga tattatgtgg acagcggcaa tcagacgttc
     1861 agttggaatg gggtggacga agaggggaac acccttgcca gcagtattta tccctacacg
     1921 ctgtccgcca tggatgcctt taacaaccat gccgtgctcg tggccgggga caactactcg
     1981 cagttcggca ccatcaccat caaccgcgtg gccacccagc cgcgcgccat ctccctggcg
     2041 gcggtctacc cggcgccgaa cgagaccgtc acctcgcgca tcaacagcgt ctacgccgtg
     2101 ctcgacccca aacgcaccga tgtgaccgtc gacggttcca tctccaccct ctatctcacc
     2161 ggccccaaca acaccaccgt catcggccag aaacgctggg acgatgcgca gaaacgcatg
     2221 gtgtattacc tcacccagcc cctcacgcag gacggccaat acaccatgaa cgtgtacgcg
     2281 cggggcaaga ccatctgggg ggacgacgtc cgggaagatt acacctcttc gttcgtcctg
     2341 cagatccccc acgccctgca ggattcctat gtcagcccca acccgctcaa gggcgcttcc
     2401 agcgccaatt tcggcatcac cctgaacagc gccgccaccg tgcggctgag gatttttaac
     2461 ctgttgggcg aggaagtcta cgaccgcacc gaatcgtttg cggccggtcc gcagatcatc
     2521 gcttggaacc tccgcaacga cagcggccag cgcgtggcca gcggggtcta catctatcgc
     2581 cttgaggtgg acggccccgg cggcagtgaa gaggtcacca agaagctggt ggtgatccaa
     2641 tgaagggcgg gaggatgaag agagcggcgg tgctgtgcct gagcctggga ctgacgggcg
     2701 cggcgttcgg gggttcgaag gacgcgggga cggcgtcggc ggtgttcctg cgggacggac
     2761 agggcgcgag ggctttgggg atgggtgggg cgtatacggc ggtgggacgc ggcgtgagcg
     2821 cgatgttctg gaaccccggg ggcctgtcgc ggacgagcca gatgggcgtg atgacgagct
     2881 acaacacgct gatcgaggac gtgaagacgc agagcgtgtc gttcttgtgc ccgctgtcga
     2941 agggggagaa gggcgcgatc ggggtgagcg cggtgatgat gaacgtgggg gacatcgaga
     3001 agcgggacgg gacggggacg cttttgggag aagggaaagt gaagggcggg gcgtatggat
     3061 tggggtatgg gttccacctg gggcgttggg gtttcggggc gcaggtgaag agcgtgaacg
     3121 aggatttgat cgcggagaag gggaaggcga cggcggtgga cgcgggggtg ttgggacagt
     3181 ggggaagcgt gtcggtgggg ctgagcgcgc agaatttcgg tgggaaatac aaggtgggag
     3241 gactcgagaa cgatttgccc ggggtcctga ggggcggggt ggcgtatcat ccggtggaga
     3301 cggagaacca atggctggag cggatgacgg tgtcggcgga tgtggagaag ccggtggacc
     3361 aggactggaa ggtgcgcggg ggcggggaat acgtgttgag ccggatgctg agcgtgcgcg
     3421 cgggatatga gcagatgagg gacctggact cgtcgggcat cagttttgga ttgggtttat
     3481 ggacggattt caagaagatg gagaaagagg agggggacta ccggaggaac acggagagcc
     3541 gggtggtgat ggaagtggac tacgcgtatc tgatgcgtga cgtcttcgac gacctccacc
     3601 gcgtcaccct ctccctgcac ttttaaa
//
LOCUS       DAOTFD010000249         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_19444_length_3598_cov_3.467965, whole genome shotgun sequence.
ACCESSION   DAOTFD010000249 DAOTFD010000000
VERSION     DAOTFD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19444_length_3598_cov_3.467965"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5079_10655"
     CDS             complement(<1..228)
                     /locus_tag="P5079_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30481.1"
                     /translation="MFGWGKKDKPATAEKPKAQKAAAPAALKPASTATKDNKSDLKVD
                     IKERKGCSAVLAIQVSAKKVEEATEDSFRRIQ"
     gene            complement(305..385)
                     /locus_tag="P5079_10660"
     tRNA            complement(305..385)
                     /locus_tag="P5079_10660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Leu,seq:tag)
     gene            complement(452..524)
                     /locus_tag="P5079_10665"
     tRNA            complement(452..524)
                     /locus_tag="P5079_10665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Lys,seq:ctt)
     gene            complement(617..690)
                     /locus_tag="P5079_10670"
     tRNA            complement(617..690)
                     /locus_tag="P5079_10670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Arg,seq:tcg)
     gene            complement(907..3363)
                     /locus_tag="P5079_10675"
     CDS             complement(907..3363)
                     /locus_tag="P5079_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRY30482.1"
                     /translation="MTNQNKFVCLHGHFYQPPRENPWLEAIEVQDSAYPHHDWNERIT
                     LECYAQNTASRILGADQNIAEIVNNYLDLSFDFGPTLLVWMEKHAPDAYRAIVESDRE
                     SVKRHGGHGNALAQVYNHMIMPLANHTDKVTQVVWGIRDFQRRFGRAPEGMWLAETAV
                     DTETLEIMAGFGIRFTVLSPGQARRVRPTGDGKVSWHDASGGRIDPTRAYRWVSPRGL
                     GMNLFFYDGPISRAIAFEGLLGNGEYLTGRLMGAFSDSAHRPQLVSIATDGESYGHHH
                     RFGDMALAYGLKKIRREQLAVITNYAEYLALHPPEWEVDIFENSSWSCAHGVERWRAD
                     CGCRIGSGPHWTQQWRGPLRESLNDLRDALDLLFEAQAGVLLKDPWQARNDYIDVLGD
                     RSPETVNRFIETHQSRPLSPSERLDVLKLMEMQRQRMFMFTSCGWFFDEISGLESVTI
                     LYSAARAIQLAQGFPNGASLEDDFLRGLARARSNIPEFMDGANVYRRLVKPLVTDLSR
                     VANHFAMGSLFQERPDQGKIYCYPYTVVDRKAGSAKGAELSVVHLRVSSEITREEADL
                     VCAVLHRTGHDFHGVVKNFTDEASYQGLKNALFEKLESGSFEDVQGVLRQQFPGAPFT
                     LQDVFLEDRRRILQSIVKDILGRFDGMYRQMISENMKLVNYLVDVSYPIPEGFQLVLQ
                     YVLDQDMEKVLENLEGNAQEIAQLTRIRREAEKFRLTLNFVPLEEKIRRRVEELMESL
                     SKNPDPEIARRVLHLLDLADRADVRPFLWRAENLFYEVWKNRLKGRLDVSRPDDPAVR
                     PYLELAGRLRFRLRPDAELL"
BASE COUNT          650 a         1121 c         1132 g          695 t
ORIGIN      
        1 ctggatccgg cggaacgagt cctcggtggc ttcctccact tttttcgcgg acacctgaat
       61 ggccaaaacc gcgctgcagc ctttgcgttc cttgatatcg accttcaggt cgcttttatt
      121 gtctttcgtc gcggtcgagg ccggcttaag ggcggccggc gccgccgctt tttgcgcttt
      181 cggtttctcg gccgtggccg gtttgtcttt tttgccccat ccaaacatgg tcgtccccca
      241 tcaaagttaa aagtattgtc gtcgcccgcc cggcacgtct tcgcatgtcc cttttcagaa
      301 agagtgcgag gggtgggact tgaacccaca agccttccgg catacggtcc taagccgtac
      361 gcgtctgccg ttccgccacc ctcgctcaaa agcaagtgct gacggggttc aactcagtcc
      421 ttcgtccttg ttgagccgtc ctgaaatatt atgggccatg cgagaatcga actcgcaacc
      481 tcgcgcttaa gaggcgcttg ctctaccagt tgagctaatg gccctgaaac cgacggatcg
      541 agagcggagt cctgcgaaaa actccgaaaa cgaagtttcg ctcggtcctc aacacccgga
      601 acccggcacc aatcatcgcg cccggaggga cttgaacccc cagcctactg gtccgaagcc
      661 agtcgctcta tccaattgag ctacgggcgc aaaacgaccg ctggtaacaa gccctgtcgc
      721 tctcccctct tggggcgaca ggtgaaaaaa tccccttccc cgccttgcat gaaaaagggg
      781 ggtcctctcc gaggccaaaa tgttctcgaa cggaccggga gggcgcgttt cccctctcgg
      841 cgggacagta taccatatcg ccccgggagg ccggaagcct ccgtccgacg aaaaaacggc
      901 gcccccttag aggagttccg cgtcgggccg gagacggaac ctcagccgtc cggccaattc
      961 caaatagggc cgcaccgccg ggtcgtccgg acgggagacg tccaaccgac ctttgaggcg
     1021 gttcttccac acctcataga aaaggttctc cgcccgccag aggaacggcc gaacatcggc
     1081 ccggtcggcc aggtccagaa gatggagcac ccgccgggcg atttccggat cggggttttt
     1141 tgaaagggac tccatcagtt cctccacacg gcggcggatc ttttcctcca acggcacgaa
     1201 attgagcgtg agacggaact tctccgcctc gcgcctgatc cgcgtgagtt gggcgatctc
     1261 ctgggcgttg ccctccaggt tctccagaac cttctccatg tcctggtcga gcacgtactg
     1321 caggaccagc tgaaaacctt ccgggatggg atagctgacg tccaccagat agttgaccag
     1381 tttcatgttt tcgctgatca tctggcggta catgccgtcg aaccggccga ggatgtcctt
     1441 gacgatggac tgcaggatgc ggcggcggtc ctccaggaac acgtcctgga gcgtgaaggg
     1501 agccccgggg aactgctggc gcaacacccc ctgaacatcc tcaaacgacc ccgactccag
     1561 tttttcgaac agggcgttct tgagcccctg ataggacgcc tcgtcggtaa aattcttcac
     1621 caccccgtgg aaatcgtgcc cggtccgatg cagcacggcg cacaccaggt cggcctcctc
     1681 ccgcgtgatt tccgaggaga cgcgcagatg gaccacggac agctcggccc ccttggcgga
     1741 acccgccttg cggtcgacca ccgtgtaggg ataacaatag atcttcccct gatcgggccg
     1801 ttcctgaaac agggagccca tggcgaaatg gttcgccacg cgggacaggt cggtgaccag
     1861 gggcttcacc aggcgacggt agacgttggc gccgtccatg aattccggga tgttgctgcg
     1921 ggcccgggcg aggccgcgga ggaaatcgtc ctccagggaa gcgccgttgg ggaacccctg
     1981 ggcgagctgg atggcccggg cggcggaata gaggatggtg acgctctcca ggccggagat
     2041 ctcgtcgaaa aaccagccgc agctggtgaa catgaacatg cgctggcgtt gcatctccat
     2101 aagtttcagc acatccagcc gttccgacgg actgagcgga cgggactggt gcgtctcgat
     2161 gaagcggttc accgtctccg gcgaccggtc gccgaggacg tcgatgtaat cgttccgcgc
     2221 ctgccagggg tccttgagca ggacgcccgc ctgggcctcg aaaagaaggt cgagggcgtc
     2281 gcgcaggtcg ttcagggatt cccgcagggg accgcgccac tgctgggtcc aatgagggcc
     2341 cgaaccgatg cggcagccgc agtcggcccg ccagcgttcc accccgtggg cgcagctcca
     2401 ggaggaattc tcgaagatgt ccacctccca ctcgggcgga tggagggcga ggtattcggc
     2461 gtagttggtg atgacggcca gctgttcgcg gcggattttt ttgaggccgt aggcgagcgc
     2521 catgtccccg aaacggtggt ggtgcccgta ggattctccg tcggtggcga tgctcaccag
     2581 ctgcggccgg tgggccgagt cggagaaggc gcccatgagc cggccggtga ggtattcgcc
     2641 gttgcccagg agcccttcga aggcgatggc ccgcgagatg gggccgtcgt aaaaaaagag
     2701 gttcatcccc aaaccgcgcg gcgacaccca acggtaggcg cgcgtcgggt cgatgcgtcc
     2761 gccggaggcg tcgtgccagg acaccttgcc gtcgcccgtc ggccggacgc ggcgggcctg
     2821 tcccggggaa agaacggtga acctgatgcc gaacccggcc atgatctcca aagtctcggt
     2881 gtccacggcg gtctcggcca gccacatccc ttccggcgcg cggccgaaac gccgctggaa
     2941 atcgcggatg ccccacacca cctgcgtcac cttgtcggta tggttggcca ggggcatgat
     3001 catgtggttg tagacctggg ccagggcgtt gccgtgcccg ccgtgccgct tgacgctctc
     3061 gcggtcgctc tccacgatgg cgcggtaggc gtccggggcg tgcttctcca tccacaccag
     3121 gagggtgggc ccgaaatcga aactgaggtc cagataattg ttcacgatct cggcgatgtt
     3181 ctggtccgcc cccaggatgc gggaggcggt gttctgcgcg taacattcga gggtgatgcg
     3241 ctcgttccag tcgtggtgcg ggtaggcgga gtcctgcacc tcgatggcct ccagccaggg
     3301 gttctccctc ggcggttgat agaagtgtcc gtgcaggcaa acaaatttat tctggttcgt
     3361 catgtcctcg tgtcctcgtt ggtggaagcg gtgtcggtgg ttgggaaacg ccggaatgcg
     3421 ctcagcgctt caggtgggcc catttgctgc ggccgcgcgg gacggccacg atgccggtct
     3481 tgtccacggt gtatttggcg gcgatgtcct tttccttgtc gaaaccgatc tccgattttt
     3541 ccgggaagga gttgaagcgg tcggcgacca cgcgcttgaa gcggcagccc ggccccac
//
LOCUS       DAOTFD010000250         3585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_19579_length_3585_cov_2.210765, whole genome shotgun sequence.
ACCESSION   DAOTFD010000250 DAOTFD010000000
VERSION     DAOTFD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19579_length_3585_cov_2.210765"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="hemA"
                     /locus_tag="P5079_10680"
     CDS             complement(<1..896)
                     /gene="hemA"
                     /locus_tag="P5079_10680"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRY30483.1"
                     /translation="MKQLVLVGLSHKTASVALRERASFAPDRTRRFLKEGILSHCFDE
                     AVVLSTCNRTELYAVGDDPGIVAADLRRTLLSPREGEEDSCYECRGMEAVSHLFAVAA
                     GLDSMVPCESEVLGQVKRAYELSRDAGATGKLTNVVFQRALFVGKLVRTRLGTRPIPS
                     SIAQVSVALAGRLFKSLAECKVLVVGAGVIAESVAGQFSSCPVSRLIVINRSPDKARR
                     LAGSVKGTDRPFDDLAKELESCDVAVFSTSSTTHLLSAEDVLSVMERRPRRPLFLIDL
                     SVPRNIDPAAHRVWDGDEEIRLT"
     gene            complement(906..1601)
                     /locus_tag="P5079_10685"
     CDS             complement(906..1601)
                     /locus_tag="P5079_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30484.1"
                     /translation="MKKMIESILISLVVVVSFVWSAQAEVKASTPSVNVSPIAPTRPV
                     RSSDLSVQNVVRVTTSVIVVEQTQTFKGEFLNVLWTVLDVDKNGKLSAEEKKNADKQL
                     KGFYLDYSGIRLNWYVMAPNKLDVDVKGMPNKKPKEAAKGAPVTVSFRVSYDYIPNFS
                     DVVELLSPNLNAHEVKTKFVLAPSLMLFRINFGKIVSPQETDGMVQVDGYPDIFTAII
                     SNQYPPHTHGDEK"
     gene            complement(1626..2507)
                     /gene="ccsA"
                     /locus_tag="P5079_10690"
     CDS             complement(1626..2507)
                     /gene="ccsA"
                     /locus_tag="P5079_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY30485.1"
                     /translation="MMFFESALFYMAFGGYLAGMALYWIPVFSRRDSSTVPAGRVVLA
                     ALLCHLVSLVLRFAVGRQMPDHQWYMPWCNWYEAFSFFAFLIAAVFAFLQIRVRLPVL
                     GLFVMTLAVAFLAASVGYPALSGDGVTMIRRIPSLPAALNSAWMLFHGPVIFLSYALF
                     ANAFGVAVAYIVQERSMKDKSFDDFSFHLPSLEELDGLVFRLISLAFPLLTLGMILGA
                     QWAFQAWGRYWGWDAKETWSLITWAVYLVYLHMRLVNGWTGGRSIYLSVAGFGAVMVT
                     SIGVNSFSAVHGFLFGG"
     gene            complement(2664..>3585)
                     /locus_tag="P5079_10695"
     CDS             complement(2664..>3585)
                     /locus_tag="P5079_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30486.1"
                     /translation="NRQRKDFPPYKRFFTWDIHHACNYRCSYCFFHETWGDEEKKNVY
                     PGLDRLMRIWDDIYDKYGESHVHVSGGEPFFYPGFIDLVAHLIQKHTFEVDTNLSFDV
                     AEWIRRIPPERTKLDVTFHPQFADLDEFYSKAKALKDAHYDIGVNYVAFPEQLSQMKQ
                     FRQVFVENGLSFDIMPFRGKYQGRDYPQGYTDSEKEMIRQCDPRTASRMLSAFDQKKT
                     PESLNPHAGKLCRMGQMYAKIMPDGTVSRCCHHRHSPLIGNLLDGTFVFYEEAKTCPE
                     TQCSCWIPMVVEQEKNWNSHWISPNERKKR"
BASE COUNT          884 a         1070 c          955 g          676 t
ORIGIN      
        1 gtaaggcgga tctcctcgtc gccgtcccac acgcgatggg cggccggatc gatgttccgg
       61 ggaacggaga ggtcgatcaa gaacaaggga cggcgaggac ggcgctccat cacggagagg
      121 acatcctcgg cactcaggag atgagtcgtg gaactcgtcg aaaaaacagc cacatcgcac
      181 gattccaact ccttcgccaa atcatcgaag ggacggtcgg tccctttcac gctcccggcc
      241 aaacgccggg ccttgtccgg cgaacgattg atcacgatca accgcgacac cgggcacgag
      301 gagaattgtc cggccacgga ctcggcgatc accccggctc cgacgacgag gaccttgcat
      361 tccgcgaggg acttgaagag acggccggcc aaagcgaccg aaacctgggc gatggaagag
      421 gggatcggcc gcgttcccag acgggtgcgc accaatttcc cgacgaaaag agcccgctgg
      481 aaaaccacgt tcgtcagttt tccggtcgcc cccgcgtccc gcgacaactc ataggcccgc
      541 ttcacctgcc ccaacacctc ggactcgcag ggcaccatgg aatccaaacc cgccgccacg
      601 gcaaaaagat gggacacggc ttccatcccc cgacattcgt aacaggaatc ctcttccccc
      661 tcgcgcggcg agagcagcgt ccgccgaaga tcggcggcca cgatcccggg atcatctccc
      721 acggcgtaaa gctccgtccg attgcaggtg gacagaacga ccgcttcgtc gaaacagtga
      781 ctgaggattc cttccttcaa gaaacggcgg gttcggtcgg gcgcgaatga cgcccgttcg
      841 cgcaaggcga cggacgccgt cttatgggaa agcccgacca aaaccagctg tttcatgaaa
      901 cccctttatt tctcgtcgcc gtgcgtatgg ggcggatatt ggttcgagat gatggccgtg
      961 aagatgtcgg gatatccatc cacctggacc atcccgtcgg tttcctgggg agacacgatc
     1021 ttcccgaaat tgattcggaa cagcatcaga gacggggcga ggacgaattt cgtcttgacc
     1081 tcgtgggcgt tgaggttcgg gctcaacaat tccaccacgt ctgaaaagtt cgggatataa
     1141 tcatagctca ccctgaacga gaccgtgacg ggggccccct tggcggcctc cttgggtttc
     1201 ttgttgggca ttcccttcac gtccacatcc agtttattcg gagccatcac gtaccagttc
     1261 aaacggatgc ccgaataatc cagatagaac cctttgagct gtttatccgc gttcttcttc
     1321 tcctcggcgg ataatttgcc gttcttatcc acatcgagga cggtccagag cacattcaaa
     1381 aattcgccct tgaacgtctg agtctgttcc acgacgatca cgctggtggt cacgcggacc
     1441 acgttctgca cgctcaggtc ggaactgcgg acaggccgcg tgggagcgat cggcgacacg
     1501 ttcacggacg gcgtggatgc cttgacttcc gcctgggccg accagacgaa ggacaccacc
     1561 accaccaaag aaataaggat tgattcgatc atctttttca tgactacctc cagtttgatt
     1621 taaaatcaac caccgaacaa gaatccatgc acggcggaaa atgagttcac tccgatggag
     1681 gtgaccatca cggcgccaaa acccgccacc gacaggtaaa tggaacgccc cccggtccac
     1741 ccgttcacca accgcatgtg aaggtagacc agatagaccg cccatgtgat cagactccaa
     1801 gtctccttgg cgtcccatcc ccaataacgg ccccacgcct gaaacgccca ttgggctcca
     1861 aggatcatcc ccaacgtcaa caaggggaac gccaaggaga tcagccggaa caccagtccg
     1921 tccaactcct ccaaagacgg gagatggaac gagaaatcgt cgaaggactt gtccttcatg
     1981 ctccgctcct gcacgatata ggccacggcc acaccgaagg cgttggcgaa cagagcgtag
     2041 gacaggaaga tgaccggccc atggaacaac atccaggcgg aattcaatgc cgccgggaga
     2101 gaagggattc gacggatcat cgtcaccccg tccccggaca gcgcgggata acccaccgac
     2161 gccgccaaaa acgccacagc caacgtcatc acgaagaggc cgaggaccgg caaccgcacc
     2221 cgaatctgga ggaacgcaaa aacagccgcg atcaagaatg cgaagaaaga gaatgcctca
     2281 taccaattac accacggcat ataccattga tggtccggca tctgtcgccc cacggcgaac
     2341 cggaggacaa gagagacgag atggcacaag agggcggcca aaaccacgcg tccggccggg
     2401 accgtcgaag aatccctccg ggaaaaaacg gggatccaat agagcgccat ccccgcgaga
     2461 tatccgccga acgccatata aaatagagcc gattcgaaaa acatcatgaa aactctcaat
     2521 acccccactc cggagcgcga cggccccgct tccaagggct attattttac ataatttaac
     2581 ggagacagac tggcgaaaaa acccctctgc gaaagccggg ggaagggcca ctttgggctg
     2641 gattaccgag aagcggcgga ctgttacctt ttcttcctct cgttcggaga aatccaatgg
     2701 gaattccaat ttttttcctg ttccacaacc atcgggatcc agcaagaaca ctgcgtctcc
     2761 gggcacgtct ttgcctcctc ataaaacacg aacgtcccat cgagaaggtt cccgatgagc
     2821 ggcgaatggc ggtgatgaca acagcgggag accgtcccat cgggcatgat cttggcgtac
     2881 atctgcccca tacggcacag cttccccgca tgggggttca aggattccgg ggttttcttt
     2941 tgatcgaatg cggacagcat gcgcgacgcc gtccgaggat cgcattgacg gatcatctct
     3001 ttttccgagt cggtatatcc ctgcggatag tcccttccct gatattttcc gcggaacggc
     3061 atgatgtcga aggagaggcc gttctcgacg aaaacctgcc tgaattgttt catctgagac
     3121 aactgctcgg ggaaagccac ataattcacc ccgatgtcat agtgggcgtc cttcagcgct
     3181 ttcgccttcg aataaaactc atcgaggtcg gcgaattggg gatggaaagt cacatcgagt
     3241 ttcgttcttt cgggagggat ccgcctgatc cattcggcaa cgtcaaatga aagattcgtg
     3301 tcgacttcga aggtatgttt ctggatgaga tgggcgacca ggtcgatgaa accgggataa
     3361 aagaacggct cgcctcccga cacatgcaca tgagattccc cgtatttatc atagatgtca
     3421 tcccagatgc gcatgagccg gtccaatccg ggataaacgt tcttcttctc ctcgtcaccc
     3481 catgtctcat ggaaaaaaca gtatgaacac cggtaattgc aggcgtggtg gatgtcccag
     3541 gtaaagaaac gcttgtatgg cggaaaatct ttccgctgtc ggttc
//
LOCUS       DAOTFD010000251         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20023_length_3532_cov_4.746333, whole genome shotgun sequence.
ACCESSION   DAOTFD010000251 DAOTFD010000000
VERSION     DAOTFD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20023_length_3532_cov_4.746333"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="P5079_10700"
     CDS             complement(<1..1711)
                     /locus_tag="P5079_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30487.1"
                     /translation="MTPLAKRHWIFLKKVTAGAVTATFLYSTLAASYAEANFWEQRRT
                     AARQQQNLTEEETAPAAKQAAPTSSDWFSRVEPSLQIPNDSGAVVNVFRGNPDQPFII
                     HLQDAHGHYSAQKQAIEILRHLQTHRKVRTVALEGAWDRLPIEWLAAFPDQNIKTRVA
                     DKYFDRGDLNGEEYLALVQPAGTWELQGIENATLYKQNVATKEETVTARAEALQHFQL
                     VQERLRKITSRLYPPALHDVERFSDAYHAQQLSFVPFTQRLIALSGRLKNPALRRADY
                     PTLTRALDLLAAESVLSLETVSREREDLIREAGDRMPEKELSALLAATVEFRTGVLSP
                     RQYHDILLQVADRLGQPVPQLREYARYLAAYETVDQDVLEQEIRRMERAVWNELATPP
                     ARRLGPVSRWVRMQLKLWSLELTPDDTAAYPQEKITWQEARDIIHDYEKELRLSASPL
                     PEPGLWLHMAEKVRKYYQLAEARNDFIVDNVLRAMKDSGQRVAVLIAGGYHTPGITKR
                     LKDRGVSYEVVRPRFTGDATPPHQKPLLGQNIEWRENLRRVMGTLRPFPNTGKVDFGV
                     NYGL"
     gene            complement(1708..2478)
                     /locus_tag="P5079_10705"
     CDS             complement(1708..2478)
                     /locus_tag="P5079_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30488.1"
                     /translation="MRVTLSGVKKGGQQFVTRLLMVTIFSQFSCLGNGFAFVQLPSSS
                     DHQCVPFFTVLLAEFLPHYKNFLPSTLPTATPFLSDVRDALFSSFGKQTAGPAEDSSL
                     VLCLSSAHAAFDNLCKRLTEEEPPAAPQGDASPEMTSSPSPAHSPSRTQFLPTKRDFF
                     LRHNGTPLASFKVNPQVSFSSDPLGLHSISPLTFHSPIWQNGRSALLQKMLVGCGRGS
                     REGDAPLALSPVFHVMPGLNQKTGHFYFRPTAWAGGRP"
     gene            complement(2654..>3532)
                     /locus_tag="P5079_10710"
     CDS             complement(2654..>3532)
                     /locus_tag="P5079_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30489.1"
                     /translation="RWSTRFHILGLRDPAAWNALRARTASSETVAEPALPPLSAPAVE
                     PLRLLSGKMPTDSLEPVAELAPRLSYFAEDGTDVVTFDWRSLKKVWAQGLFQLMEAHL
                     AQAEQSGKPNKITFAFVSDVPGLSSEQMLDALQLHGLPERVKKSLLLVDQATLKDRGV
                     LRKGAIQVQKLSLVMGEMLLHRWNTGLLRLKVVVDSENAHRWQGDVLKVLLTPAGTGE
                     IVSTSLGLMVAIEGEMSDRIVTFIRQYYPAEVAERLLSRIQKSAGRILLPATTTTPQQ
                     DLPGKLKNQQILDTQA"
BASE COUNT          660 a          951 c         1218 g          703 t
ORIGIN      
        1 ccagcccgta attgacgccg aagtccactt tgccggtatt ggggaaagga cgcagggtgc
       61 ccatgacccg gcggaggttt tccctccatt cgatgttctg tcccagaagc ggtttctggt
      121 ggggcggggt ggcgtcgccg gtgaaacggg gccggacgac ttcataggag acgccgcggt
      181 ccttcaggcg tttggtgatg ccgggggtgt gataaccgcc ggcgatgagc accgccacgc
      241 gctggccgga gtctttcatg gcgcgcagga cgttgtctac gatgaaatcg ttgcgggctt
      301 cggcgagttg gtaatatttc cgcaccttct ccgccatgtg gagccacaga ccgggttccg
      361 gcagcgggga ggcggaaaga cggagttcct tttcgtagtc gtggatgatg tcccgcgcct
      421 cctgccaggt tattttttcc tgaggatagg cggcggtatc gtccggggtg agttccaggc
      481 tccagagttt caactgcatg cgcacccagc gggaaacggg acccagccgt cgggccggcg
      541 gggtggccag ttcgttccag acggcccgtt ccatccggcg gatctcctgc tccaatacgt
      601 cctggtcgac ggtctcgtag gccgccaaat aacgggcgta ttcgcgcagt tgagggacgg
      661 gctgcccgag tcggtcggcc acctgcagga ggatatcgtg gtattgccgc gggctgagca
      721 cgccggtgcg gaactccacg gtggcggcga ggagggcgga aagctctttt tccggcatgc
      781 ggtccccggc ctctcggatg aggtcttcgc gttcgcggga aaccgtttca agggatagga
      841 cgctttccgc ggccagaagg tcgagcgccc gggtgagcgt gggataatcc gcccggcgaa
      901 gggccgggtt cttcagccgg ccggagaggg cgatgagacg ttgggtgaac gggacgaagg
      961 aaagctgttg cgcgtgatag gcgtcggaga agcgttccac gtcgtgcagc gccggcgggt
     1021 agaggcgcga ggtgatcttt cgcaggcgtt cctgcacgag ctgaaagtgc tgaagggcct
     1081 cggcccgcgc ggtgacggtt tcctccttgg tcgccacgtt ctgtttgtag agggtcgcgt
     1141 tttcaatgcc ttgcagttcc cacgtccccg ccggttggac gagggccaga tattcttcgc
     1201 cgttgaggtc gccccggtcg aagtatttgt cggccacgcg ggtcttgatg ttctggtccg
     1261 gaaacgccgc cagccattcg atggggagcc ggtcccacgc gccttccagg gccacggtgc
     1321 ggactttgcg gtgggtctgg aggtggcgga ggatctcgat cgcctgtttc tgggcgctgt
     1381 agtggccgtg ggcgtcctgc agatggatga tgaagggctg gtcggggttc ccacggaaca
     1441 cgttgacgac ggcgccggag tcgttgggga tctgaaggct cggttccacg cgcgagaacc
     1501 agtcgctcga ggtcggagcg gcctgtttcg ccgcgggggc ggtctcttct tcggtgaggt
     1561 tctgttgctg acgcgccgcg gtgcggcgtt gttcccagaa attggcttcc gcgtaagagg
     1621 cggccagagt gctgtaaaga aacgtggcgg tcaccgcgcc ggcggtgact tttttcagga
     1681 aaatccagtg tctcttcgcc agcggcgtca cggccgccct cccgcccacg ccgtcggacg
     1741 gaaataaaaa tgcccggttt tttggttcag accgggcatg acatgaaaaa cgggggacaa
     1801 cgccaacggg gcgtctcctt ctcggcttcc ccgtccgcat cccaccagca tcttctgaag
     1861 cagcgccgac cggccgtttt gccaaatcgg cgaatggaag gtcaacggag aaatcgagtg
     1921 aagtccgagg gggtcggagg agaacgaaac ctgcgggttg accttaaaag aagccagcgg
     1981 cgttccgttg tggcggagaa agaaatcccg tttggtcggc aggaactgcg tccgcgacgg
     2041 cgagtgggcc ggcgaggggg aagacgtcat ttccggggac gcatcgccct ggggggcggc
     2101 cggaggttcc tcttccgtca gacgtttgca gaggttgtcg aacgcggcat gggccgatga
     2161 aaggcacagc acgagggagg agtcctcggc cgggcccgcg gtttgtttcc cgaaggacga
     2221 aaaaagggcg tcccgcacgt ccgagaggaa cggggtcgcc gtcggcaacg tcgacggcag
     2281 gaaatttttg tagtgcggca ggaattcggc caggagaacg gtgaagaacg gaacgcattg
     2341 atggtcggat gaggacggga gttggacaaa agcaaaacca tttcccaaac aggagaattg
     2401 ggaaaagatt gttaccatga ggagtctggt aacgaactgc tgcccgcctt ttttgacgcc
     2461 gctcaacgtc accctcattt gcccaccttt gttatagagg acagaaggcc ttttgtccag
     2521 gcttttctaa aaaaagtatg acaaagaggg ataatccgag agttaaggat ttgtaaacgg
     2581 aatgtaaatt attcaaccgc ttgaacagag ggagaaaaaa actttttgaa taaagggaat
     2641 gaagcggaaa cccttaggcc tgggtatcga gaatctgctg gtttttcagc ttgccgggga
     2701 ggtcttgttg cggtgtcgtt gtggtggccg gcagcaggat ccggcccgcg cttttttgaa
     2761 tgcggctgag aagtcgttcg gcgacctcgg ccgggtaata ttgacggata aaggtcacga
     2821 tccggtccga catttccccc tcgatggcca ccatcagccc cagagaagtg gaaacgatct
     2881 ccccggtgcc ggcgggggtg agcagcactt tcagcacatc gccctgccaa cggtgggcgt
     2941 tttcgctgtc caccaccacc ttcaagcgca acagcccggt gttccagcga tggaggagca
     3001 tttcccccat caccagggac agtttttgca cctggatggc gcctttgcgc aacaccccgc
     3061 gatcttttaa ggtggcctgg tccacaagga gaagggactt cttgacgcgc tccggcaggc
     3121 cgtggagctg gagggcgtcg agcatctgtt cggacgacag accgggcacg tccgacacaa
     3181 aggcgaaggt gatcttgttg ggtttgccgg actgttcggc ctgggccaga tgcgcttcca
     3241 tgagttggaa gaggccttgc gcccagactt ttttgaggct gcgccagtcg aaggtcacca
     3301 catcggtgcc gtcctcggcg aaataggaaa gccgcggggc cagttccgcg acgggttcca
     3361 ggctgtcggt cggcattttc ccggaaagga gccggagcgg ctcgacggcc ggtgcggaga
     3421 ggggcggcaa ggccggttcg gcgacggttt ccgaggaggc cgtgcgggcg cgcagcgcgt
     3481 tccacgcggc gggatcgcga agacccagga tatggaaacg ggtgctccat cg
//
LOCUS       DAOTFD010000252         3531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20030_length_3531_cov_4.324799, whole genome shotgun sequence.
ACCESSION   DAOTFD010000252 DAOTFD010000000
VERSION     DAOTFD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20030_length_3531_cov_4.324799"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5079_10715"
     CDS             complement(<1..553)
                     /locus_tag="P5079_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY30490.1"
                     /translation="MENIGSIETFLKTLYEKGASDLHLKAGRPAMMRLRGDLLPMENV
                     PALQSEDVQKLIYSVISAEQQKRLEEDKELDFSFNVRGMARFRGNAFFQKGALGAVFR
                     LIPAKIPTLDELGMPPVLKELVNRKQGLFLVTGPTGSGKSTTLAALIDQINNTMPVHV
                     VTLEDPIEFSYQDKMAVINQREVG"
     gene            complement(567..1667)
                     /locus_tag="P5079_10720"
     CDS             complement(567..1667)
                     /locus_tag="P5079_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY30491.1"
                     /translation="MEFIDVLRLGVKQGASDIHLVIGKPPMARVNGEILELPGFEPVS
                     AEESKRLIYSILYDEQKQRFEEQLELDCSFAIAGLARFRVNVLMQRNGVEAVLRIISS
                     KIPDPETLRLSPAILEFAKLPRGLVLVTGPTGSGKSTTLACLVNIINSVRREHILTIE
                     DPIEFVYEHNKCIVRQREVGQSTRNFNEALRHALRQDPDVILVGEMRDLETISLAITA
                     AETGHLCFGTLHTTDAPQTVDRIIDVFPPHQQQQVRVQLSTVLKGVVCQTLIPRLEGE
                     GRVAAREIMIVTPAIANLIREGKTHMIYNAIETGAKFGMVPLDRCLADLVGEGVISFD
                     DALTKSSDPEKLRNLTGRADAGSPVSSGSGTY"
     gene            complement(1704..2912)
                     /locus_tag="P5079_10725"
     CDS             complement(1704..2912)
                     /locus_tag="P5079_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRY30492.1"
                     /translation="MPEKDNSRRPMAPLRLVALVGLFLVVASCFLAVRSPVAAALRSG
                     EPLSGILLGTDFVETAQHSDTLMLWYYSPRRAHLDVLSLPRDTRINLPGFRFHRINEV
                     FAYHFALKRDPRFASGEVLKAVAYLLNFKPIYYLHVNFDGFRRFIDLIGGLNVHIDEP
                     LHYDDNAGDYHFHREPGDYHLSGDEALAYVRFRGRSGDRGRILRQMEFLKGVAEKISF
                     PLLAFRWPKMLATVYGSVHTNLGLWDVPFLVLEAKHLRPDRLNPWLLPGQNRGVYWEM
                     DVERTGLVLRQITGQENGSAEAVAEEEPVSPAATHAKVKVWNGSGRSGLALKVTRRLR
                     AGGFDVLEWGNYNLRQTKTRVLDRCGDIDKARGVARLLGVESVFSDINPKLQTDVEVI
                     LGEDYTGETR"
     gene            complement(2916..>3531)
                     /gene="yqeK"
                     /locus_tag="P5079_10730"
     CDS             complement(2916..>3531)
                     /gene="yqeK"
                     /locus_tag="P5079_10730"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRY30493.1"
                     /translation="PPRRVISAMKKESTEDFAALDRRLRADLSPARYRHSVSVARLAE
                     RLARRHGGDLRRARLAGLLHDCARDFSAVRLAGEARRKDWLGGFVPGPGRFVLLHAPV
                     SALRARKIYGVRDRAVLAAIERHTLGHRRMGRLDKILYVADYASPDRGFPASAKMRRL
                     ALKNLEQAFRETMRQKLLYVLRSGRALHLETVKMYNASAGRKGR"
BASE COUNT          631 a         1169 c         1083 g          648 t
ORIGIN      
        1 accccacctc ccgctgattg atcaccgcca tcttgtcctg ataggagaac tcgatggggt
       61 cctccagcgt gacgacgtgc acgggcatgg tgttgttgat ctggtcgatg agcgccgcca
      121 acgtggtgga tttgcccgac cccgtggggc cggtcaccag gaacagcccc tgtttcctat
      181 tcaccaactc tttcaacacc ggcggcattc cgagttcgtc cagcgtgggg atctttgccg
      241 gaatcaggcg gaacaccgcg cccagcgcgc ccttctgaaa aaaggcgttg ccgcggaacc
      301 gcgccatgcc gcgcacgttg aaggagaagt ccagttcctt gtcctcctcc agccgcttct
      361 gctgttccgc gctgatgacc gaatagatga gtttctgcac gtcttcggac tgaagcgcgg
      421 gaacgttctc catgggcaag agatccccgc gcagccgcat catcgccggc cggccggcct
      481 tgaggtgcag gtcgctggcg cctttttcgt aaagggtctt taaaaacgtc tcgatgcttc
      541 cgatattttc catgaggcct cccggctcaa taggtgccgc tgcccgacga tacgggcgaa
      601 ccggcgtcgg cgcggcccgt caggttccgc agtttctccg gatcggacga cttcgtcaaa
      661 gcgtcgtcga aactgatgac cccttcgccg accaggtcgg ccaggcagcg gtccaggggg
      721 accatgccga acttggcgcc ggtttcgatg gcgttgtaga tcatgtgggt cttcccctca
      781 cggatgaggt tggcgatggc cggcgtcacg atcatgatct cccgcgccgc cacacggccc
      841 tccccctcca ggcgggggat gagcgtctgg cacaccaccc ccttgagcac cgtggagagc
      901 tgcacccgca cctgttgctg ctggtgcggc gggaaaacgt cgatgatgcg gtccaccgtc
      961 tgtggggcgt cggtggtgtg cagggtgccg aaacagaggt gcccggtttc ggcggcggta
     1021 atggccagag aaatggtctc caggtcccgc atttcgccca cgaggatgac gtcggggtcc
     1081 tgccgcaggg cgtgccgcag cgcctcgttg aagttgcggg tggactgccc cacctcgcgc
     1141 tggcgcacga tgcacttgtt gtgctcgtag acgaactcga tggggtcctc gatggtgagg
     1201 atgtgttccc gtcggacgga gttgatgatg ttgacgaggc aggcgagggt ggtggacttc
     1261 ccggaccccg tggggcccgt caccaacacc agcccgcgcg gcagtttggc gaattccaag
     1321 atggcgggcg aaagacggag ggtttccggg tcggggatct tggacgagat gatccgcagc
     1381 accgcctcca cgccgttcct ctgcatcagc acgttcacgc ggaaccgcgc caggccggcg
     1441 atggcgaagg aacagtccag ttccagttgt tcctcaaaac gctgtttttg ttcgtcgtag
     1501 aggatggagt agatgaggcg tttcgattcc tcggccgaaa ccggctcgaa ccccggcagt
     1561 tccaatattt cgccgttcac ccgggccatc ggcggcttgc cgatgacgag atggatgtcc
     1621 gatgccccct gcttgacgcc cagccttaaa acatcgatga actccatggt tcctccttga
     1681 acggactctc tttcgccggc cctctaccgc gtttcgccgg tgtaatcctc gcccaaaatc
     1741 acttccacgt cggtctgcag tttcggattg atgtcgctga aaacggactc cacgcccagc
     1801 agccgcgcca cgccccgggc cttgtcgatg tcgccgcagc ggtccagcac gcgcgtcttg
     1861 gtttgccgta gattataatt cccccattcc aagacgtcaa acccgccggc gcgcaggcgc
     1921 cgcgtcacct tcaacgccag tcccgaccgg ccgctgccgt tccacacctt caccttggcg
     1981 tgggtcgccg ccggagacac cggctcctct tccgccacgg cctccgccga tccgttctcc
     2041 tgtccggtga tctgccggag gaccaacccc gtccgttcga cgtccatctc ccaatagacc
     2101 cctcgattct gaccgggaag gagccagggg ttgagccggt ccggacggag gtgtttggcc
     2161 tccaacacca ggaagggcac atcccacaga cccaggttgg tgtgcacgct gccgtagacc
     2221 gtggcgagca tcttcggcca gcggaatgcc aggagcggga aagagatctt ctccgccaca
     2281 cctttcagaa actccatctg ccgcagtatc cggccgcggt cgccgctgcg gccgcggaac
     2341 cgcacgtagg ccagcgcctc gtcgccgctc aagtgatagt cgccgggctc gcggtggaaa
     2401 tgatagtccc cggcgttgtc gtcgtagtgg agcggctcgt cgatgtgcac gttgaggccg
     2461 ccgatgaggt cgatgaatcg ccggaaaccg tcgaaattca cgtgcaggta gtagatcggc
     2521 ttgaagttca gaagataggc caccgccttg agcacctcgc cggaggcgaa ccgggggtcc
     2581 cgcttcaatg cgaaatgata ggcgaacacc tcgttgatgc ggtggaaccg gaaccccggc
     2641 aggttgatgc gcgtgtcgcg gggcaaagag agcacgtcga ggtgcgcccg ccgggggctg
     2701 taataccaaa gcatgagcgt gtcggaatgt tgggcggtct cgacgaaatc cgtgcccagc
     2761 aatatccccg aaaggggctc gccggaccgc aacgccgcgg ccaccggcga ccgcaccgcc
     2821 aaaaagcagc tcgccaccac caagaacaac cccaccaagg ccaccaggcg caggggggcc
     2881 atcggacggc gcgaattgtc cttctcaggc acggctcatc ttccttttct tcccgccgac
     2941 gcgttataca tcttcaccgt ttccagatga agggcgcggc cggaccgcag aacgtacaac
     3001 aatttctgcc tcatggtttc acggaacgcc tgctccaaat ttttcaacgc caaccggcgc
     3061 atcttcgcgg aggccgggaa cccgcggtcg ggcgaggcgt aatccgccac gtagaggatc
     3121 ttgtccagcc ggcccattcg acgatgcccc agcgtatgac gctcgatggc ggccagcacc
     3181 gcccggtcgc gcacgccgta tatcttcctc gccctcagcg ccgagaccgg cgcgtgcaag
     3241 agaacgaacc gccccggccc gggaacgaaa ccgccaagcc aatctttccg gcgcgcctcc
     3301 ccggccagcc gcacggcgga gaagtcgcgg gcacaatcgt gcagcaggcc ggccagccgg
     3361 gcccgccgga ggtccccgcc gtgccttcgc gcgagccgtt ccgccagccg cgccacgctc
     3421 acgctgtgcc ggtaacgcgc cggagaaagg tcggcccgaa gccggcggtc cagcgcggcg
     3481 aaatcctctg tcgattcttt tttcatcgcc gatataaccc ggcgcggcgg a
//
LOCUS       DAOTFD010000253         3521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20125_length_3521_cov_4.480092, whole genome shotgun sequence.
ACCESSION   DAOTFD010000253 DAOTFD010000000
VERSION     DAOTFD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20125_length_3521_cov_4.480092"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="P5079_10735"
     CDS             complement(<1..598)
                     /locus_tag="P5079_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRY30494.1"
                     /translation="MAYTPDKRNQDEAERRFYAGAAVDPSPRIRLEDGSSELTMRVMD
                     LVSPIGMGQRGYIVAPPGSGKTTFLKHICQAVAKSRPEMKLYCLLINERPEEVTDFKR
                     SVPAEVWASSSDESYDHHVQMADTLVRQAVREAGGGQNVMIVIDSLTRLARVHNAQRS
                     ASGRTLSGGLDAGALEIPRRIFGAARKIEAGGSLTILAT"
     gene            732..1214
                     /locus_tag="P5079_10740"
     CDS             732..1214
                     /locus_tag="P5079_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30495.1"
                     /translation="MKMTHVFSAAFLCAALVLPGRCAEEGNEALKRWRQAERQKSDLC
                     VDKLVRTLKLTDEQSTALQNAYDERLNKLAELREKFMKDTQAVEAEADRKAVASLPPE
                     QRDKYAAAKEKIEREEREKEMQLRRPPNGEDGPFGRGGPGGGRRGGPGGFGTPPGSGY
                     "
     gene            1443..1808
                     /locus_tag="P5079_10745"
     CDS             1443..1808
                     /locus_tag="P5079_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30496.1"
                     /translation="MDRFINYLRYAARTCLVLVSSFWFVVALLSGAERYGGGLYGIFR
                     NSPNALPWLVLFVLVYVSWRWERVGGILVAALGVVTVFFFNAADSPPVLWAVSVPLFV
                     CGVFLGLGGHLKKRRKSGA"
     gene            complement(1827..2495)
                     /locus_tag="P5079_10750"
     CDS             complement(1827..2495)
                     /locus_tag="P5079_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HRY30497.1"
                     /translation="MKKIAKIVGGGAVAGFVLAAAVFLTFVLSGVPTRGPRIGHYAHP
                     RKALLVVDVQEDFIGTTCPPTRKYKNSDALIATINKTIEVSRARNYPVVYIRQEYEGL
                     WARTIAKTLFGGRGLPGRPGTQIDKRVAVVSDAIFPKRIGDAFSNPSLSDFLVKNQVD
                     ELILTGLDAQHCVHATARGALNRGYKVTAVTDALALMAENKWDALMKDYREEGILLTS
                     SGAL"
     gene            complement(2593..>3521)
                     /locus_tag="P5079_10755"
     CDS             complement(2593..>3521)
                     /locus_tag="P5079_10755"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRY30498.1"
                     /translation="LFFQYREKRGEWTPPPALARLWAYNVEILHFVYGGLLSVYVVLY
                     FQSGAGRRTAVFLFLVGALFVMNEFPLLRRNSHRLRLALYAFCVCSYLIYLVPILVGR
                     LGGWVFGASVAISVGLLWSMAGLLTRREPARRKAQRRLFAPAAGVFGLIVLLYFLHLI
                     PPVPLSVQWAGIFHDLEKTSEGYLLKTPRPPWHAFWKRESRPFRARPGDKIFYFVRIF
                     APARFEHAVHLRWYVYDAAAKRYVSSDRMLVTVIGGRRKGFRGYAYKTNYHPGRWRVV
                     TETSDGRAIGITDFEVLADKGTGPREWKAVRS"
BASE COUNT          650 a         1087 c         1138 g          646 t
ORIGIN      
        1 ccgtcgccag gatcgtcagc gagccgccgg cctctatctt gcgcgccgcg ccgaagatcc
       61 gccgggggat ttccagcgcc cccgcgtcca gcccgccgga gagcgtccgg ccgctggccg
      121 aacgttgggc gttgtgcacc cgcgccagcc gcgtcaacga atcgatcacg atcatcacgt
      181 tctggccgcc gccggcctcc cggacggcct gtcgcaccag cgtgtccgcc atctgcacgt
      241 ggtggtcgta gctttcgtcc gaggaggagg cccacacctc cgccggcacg ctccgtttga
      301 agtccgtcac ttcttccggc cgttcgttga tgagcaggca atagagcttc atctccggcc
      361 ggctcttcgc caccgcctgg caaatgtgtt tcaggaaagt cgtcttgccg gaaccgggcg
      421 gcgccacgat gtatccgcgt tgtcccatgc cgatggggct caccaggtcc atcacccgca
      481 tggtcagctc cgaactgccg tcctccagcc ggatgcgggg cgacgggtcc accgccgcgc
      541 cggcatagaa ccggcgttcg gcctcgtcct ggttccgttt atcgggagtg tacgccatat
      601 cggtatgata ttacacgtcc cgtcgggtct ccgacaactg ttttttcgat tttgcgccac
      661 cgcgttgcga cactttttca ctccccccgg cgtctgatag atgaggcaca atccaaaatg
      721 aggagaacag aatgaaaatg acccatgttt tttcggcggc tttcctatgc gcggccttgg
      781 tcctgcccgg tcgttgcgcg gaggagggga acgaggcgct caaacgttgg cgccaggcgg
      841 aacggcagaa atccgatctg tgtgtggaca agcttgtccg caccctcaaa ttgacggacg
      901 aacaaagtac cgcccttcag aacgcgtatg atgaacgcct caacaaactg gccgaactgc
      961 gcgagaagtt catgaaggac acgcaggccg tcgaagcgga ggccgaccgg aaggccgtgg
     1021 cctccctccc cccggagcag agggataaat atgccgccgc caaggaaaaa atagagaggg
     1081 aagagcgcga gaaggaaatg cagctccgcc gtccgccgaa cggcgaagac ggcccgttcg
     1141 ggcgtggcgg ccccgggggc gggagacgcg gggggcccgg cgggttcggc acgccgcccg
     1201 gaagcggtta ctgacgccgc ttcattcggc ggaaaaagaa ccgctcccat cgtcacactt
     1261 gcagacgccg cgggggggtc tccccgccgg ttttgtcccg gtcgacttct ttgccgttag
     1321 gaaaacctgg ccggcctgtc gcgtcggggg tataatgggg gcatgcggaa agaccgggtg
     1381 atccatgatt cgtccggcgt ccggccaaaa accgcgccgg cgcggccgtg cccttctgag
     1441 atatggatag gttcataaat taccttcgat acgccgcccg gacctgcctg gtgctggtgt
     1501 cgtcgttctg gttcgtcgtc gccctgttgt cgggcgcgga acggtacggc ggcgggctct
     1561 atgggatctt ccggaacagt cccaacgcgt tgccgtggct ggttttgttc gtgttggtgt
     1621 atgtgtcctg gcgatgggaa cgcgtggggg ggatcctcgt ggcggctctg ggggtcgtga
     1681 cggtgttttt tttcaacgcg gcggattcgc cgcccgttct gtgggcggtg tcggtgccgc
     1741 tgttcgtttg cggggtgttt ttgggcctcg gcgggcactt gaaaaaaaga agaaagagcg
     1801 gcgcgtaatc cgttcccggt ccggcgttac agcgccccgc tggacgtcaa aagaatccct
     1861 tcctcccgat agtctttcat caaagcgtcc cacttgttct ccgccatcaa cgccagggcg
     1921 tccgtcaccg cggtgacctt gtagccgcgg ttcaacgcgc cccgggcggt ggcgtggacg
     1981 cagtgttggg cgtccagccc ggtcaagatc aattcatcca cctggttctt caccaggaaa
     2041 tcgctcagcg aggggttcga aaaggcgtcg ccgatgcgtt tgggaaagat cgcatcggac
     2101 accacggcca cccgcttgtc gatctgcgtg cccggccgac cgggcaatcc gcgtcctccg
     2161 aaaagggttt tggcgatggt tcgggcccaa aggccttcat actcctgccg gatgtagacg
     2221 acgggataat tccgcgcgcg ggagacctcg atggttttgt tgatggtcgc gatgagcgca
     2281 tcggaatttt tgtatttccg ggtcggcggg caggtcgttc cgatgaaatc ctcctgtacg
     2341 tcgacgacca acagcgcctt ccgggggtgc gcataatgtc cgatgcgggg gccccgggtg
     2401 gggacgccgg aaaggacgaa ggttaagaac accgcggccg ccaggacgaa cccggcgacg
     2461 gctcccccgc cgacgatctt tgcgattttt ttcatggacg actcctgcgc gaattggaag
     2521 gctgatacag gattttaaga taatccgagg tccttcccag gcggcgggcg gggctgaaaa
     2581 ccgcggagtt ttttacgacc ggacggcttt ccattcccgc ggaccggtcc ccttgtccgc
     2641 aagcacctcg aaatccgtga tcccgatggc ccggccgtcg gacgtctccg tcacgacgcg
     2701 ccatcggccc gggtgatagt tcgttttgta ggcgtatccc cggaagccct tccgccggcc
     2761 gccgatcacc gtcaccaaca tgcggtcgct ggagacgtaa cgtttcgccg cggcgtcgta
     2821 gacgtaccag cggaggtgca ccgcatgttc gaaccgggcc ggcgcgaaga tgcggacgaa
     2881 atagaagatc ttgtccccgg gccgggcgcg gaaggggcgg gattcccttt tccagaacgc
     2941 atgccagggc ggccggggcg ttttgaggag atagccctcg gaggtcttct ccaggtcgtg
     3001 gaatatcccg gcccattgaa cggaaagggg caccggcggg atgaggtgca ggaaatacaa
     3061 aagaacgatg agcccgaaca cgccggccgc gggcgcgaac agccgccgct gtgcctttcg
     3121 ccgggccggt tcccgccggg tcaaaagacc cgccatggac cacaggaggc cgaccgaaat
     3181 cgccacggac gcaccgaaca cccatccgcc cagacgcccg accaggatcg gcaccaaata
     3241 gatcaggtag gagcacacgc agaaggcgta gagggccagc cgcagccgat ggctgttgcg
     3301 acggaggagg gggaattcgt tcatcacgaa gagagcgccc accaggaaca agaacacggc
     3361 tgtgcggcgg ccggcgccgc tctggaaata caggacgaca tagacgctca gcaaccctcc
     3421 atacacgaaa tggaggatct ccacgttgta cgcccacagg cgggcgagcg cgggcggcgg
     3481 ggtccactcc ccgcgcttct cccggtattg gaagaagaga a
//
LOCUS       DAOTFD010000254         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20176_length_3516_cov_3.268420, whole genome shotgun sequence.
ACCESSION   DAOTFD010000254 DAOTFD010000000
VERSION     DAOTFD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20176_length_3516_cov_3.268420"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5079_10760"
     CDS             <1..270
                     /locus_tag="P5079_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRY30499.1"
                     /translation="SPYAPLRTLLKRRVKQILLIGEAAPKIRRELDGVVPLVSCGTLE
                     KAVRTAVGAAAPGDTVLLSPACASFDQFENFEHRGRRFREIVESL"
     gene            286..1413
                     /gene="ftsW"
                     /locus_tag="P5079_10765"
     CDS             286..1413
                     /gene="ftsW"
                     /locus_tag="P5079_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRY30500.1"
                     /translation="MARKSTQPPDWVIGALTLSLLFFGWVILYSASALVAESRYGDQY
                     FFLKKQMLWSCLGMAALLAALRVKMSFVQRLARPLFVVELVFLVLVLVVGHEVAGGRR
                     WLRLAGFGFQPSEFAKLALVLVLADFLDRKKSRLGEFKSGFLPPMVLVGLVLGLIALE
                     RDLGTPMLIAMVSLGLIYLAGARYRHLLALVLAGLPVVYYAIFSVAYRRERFFTFLDP
                     WKNPQGTSYQLVQSLLAIGSGGFWGRGSGESTIKMYYLPEAQTDFVFSILGEELGFVG
                     ASLLVLLFLLLVLRCYQVAIKASNWFETLVAAGISLMLGGQVLINLGVVTGLLPTKGI
                     PLPFVSFGGSSLLVTMAAIGLVLNISRRAGEGVALQRRQLL"
     gene            1439..2521
                     /gene="murG"
                     /locus_tag="P5079_10770"
     CDS             1439..2521
                     /gene="murG"
                     /locus_tag="P5079_10770"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRY30501.1"
                     /translation="MRVCIAAGGTGGHLLPGVAVGKELRARGHEVHFVVRKDRSSQER
                     LAREGFPSSAFAYAGFPRALSLRALIYPGLSLAAYLNARRILRREDPDVVLGMGGYIS
                     VPVGLAARRADIPLVLHEQNSYAGLANRWLARWASAVATTFPETAGLPPQARRRWTGL
                     PLRPEIMPRDRRACRAGLGLSPDLFTVLIFGGSQGARALNRLAASSLAPLSEFRDRCQ
                     FIHVAGEADADVMEQVYADAGWNHFVRPYWEDIATLYAAADFVVSRSGANTVMELAYL
                     GKKALLVPFPFATNDHQRLNAAFLAKSGRAEVVLEKDLSPGRFREILKQAFVSPLEAE
                     AAPPKDFPRATASLADLLEDVRFGKT"
     gene            2550..3326
                     /gene="surE"
                     /locus_tag="P5079_10775"
     CDS             2550..3326
                     /gene="surE"
                     /locus_tag="P5079_10775"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRY30502.1"
                     /translation="MKRQPKRGSKPSIFISNDDGIYGPGLKPLVRALKSLGRVTVVVP
                     ELERSAGSHAITLHKPIRVHRLDRSTLIMNGTPADCVRFGVLSLLKEDVDVVVSGINR
                     GPNLGADTIYSGTVGAAMEGCMMGIPSLAVSLADVGRGNYETAARFTRALVKVLLERP
                     VPKDICYNVNVPDRPFGRMRGVSVTHLGKRIYGKDLESGIDPRGQAYYWLAGETPTGI
                     AVPGSDIAAIQQGKISVTPLRLDMTATEMIPVLRQWSWRY"
     gene            3395..>3516
                     /locus_tag="P5079_10780"
     CDS             3395..>3516
                     /locus_tag="P5079_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30503.1"
                     /translation="MDKFKVVSKFKPAGDQPEAIRQLVEGLRQKQCHQVLLGVTG"
BASE COUNT          530 a          984 c         1234 g          768 t
ORIGIN      
        1 tctccttacg ctcctttgcg gaccctgttg aaaaggaggg tcaagcagat cctcctgatc
       61 ggcgaggcgg cgccgaagat acggcgcgag ctcgacggtg tcgtcccgct cgtgtcctgc
      121 ggcacgctgg aaaaagccgt gcggacggcg gtcggggcgg cggcgccggg ggacaccgtc
      181 ctgttgtcgc ccgcctgcgc ctcgttcgac caattcgaga atttcgagca ccggggccgg
      241 cgcttccggg agatcgtcga gtctctctga tcgtcgggag ggtcgttggc tagaaagtcc
      301 actcagccgc ccgattgggt catcggcgcc ctcacgctga gcctgttgtt tttcggctgg
      361 gtgatcctgt attcggccag cgcgctggtg gcggaaagcc gttacggcga ccagtatttt
      421 ttcctcaaaa aacagatgtt gtggtcctgt ttggggatgg cggccctgtt ggcggcgttg
      481 cgggtgaaga tgagtttcgt gcagcgtctg gcgcgcccgc tgttcgtcgt ggagcttgtt
      541 ttcctggtgt tggttctggt cgtcggtcac gaggtggcgg gcggtcggcg ttggttgcgg
      601 ctggcgggtt tcgggtttca gccgtccgag ttcgccaagc tggcgctggt gctggtgctg
      661 gccgatttcc ttgacaggaa aaagagccgg ctgggggaat tcaaaagcgg ttttctgccg
      721 cccatggtgc tggtcggcct tgtgttgggg ttgatcgcgt tggaacggga cctcggcacg
      781 ccgatgttga tcgccatggt ttccctcggg ctgatctatc tggcgggcgc gcggtaccgg
      841 catctgttgg ctttggtgtt ggcgggcctc ccggtggtct actacgcgat cttttcggtc
      901 gcctatcggc gggagcgttt ttttactttt ctggatccct ggaagaaccc gcaggggacg
      961 agctatcaac tggtgcagtc gttgttggcc atcggctcgg gcggtttttg ggggcgcggt
     1021 tcgggcgaat ccaccatcaa gatgtactat ttgcccgagg cccaaacgga tttcgtcttc
     1081 tccatcttgg gggaggaact gggatttgtc ggcgcgtccc tgttggttct cctttttttg
     1141 ttgttggtgc tccgctgcta tcaggtcgcc atcaaggcgt cgaattggtt tgagacattg
     1201 gtggcggccg ggatcagcct tatgctcggc ggccaggtgc tgatcaacct gggcgtggtg
     1261 acggggcttt tgccgacgaa gggcatcccc ctgccgttcg tgtcgttcgg cgggtcctcg
     1321 cttttggtga cgatggcggc catcgggctg gttttgaaca tctcccgccg ggcgggcgag
     1381 ggcgtggcgc tccaacggcg gcaattgctc tagggagaga ggtcgaaggg tggcgaagat
     1441 gcgcgtgtgt atcgcggccg gcgggaccgg cggtcatctc ctgcccggcg tggcggtggg
     1501 aaaagagctg cgcgcccgcg ggcatgaggt gcattttgtg gtccgcaagg accggtcttc
     1561 ccaggaacgg ctggcccgcg aagggtttcc gtcgtcggcg ttcgcctatg ccgggttccc
     1621 gagggctctt tctcttcggg ccttgatcta cccggggttg tccttggccg cctatctgaa
     1681 cgcgcggcgg atcctccggc gggaggatcc ggacgttgtg ctggggatgg ggggatacat
     1741 ttccgtgccg gtcggcctgg cggcccgtcg cgccgatatc cccttggtgt tgcacgaaca
     1801 gaacagttac gcggggctcg ccaaccgatg gttggcgcgg tgggcgtcgg cggtggccac
     1861 cacttttccg gagacggcgg gactgccgcc ccaagcgcgc cggcgttgga cgggtctgcc
     1921 tctccggccg gagattatgc caagggatcg ccgggcctgc cgcgcgggtc tggggttatc
     1981 gccggatctt ttcacggtgt tgattttcgg gggcagtcag ggggcgcgcg ccctcaaccg
     2041 gctggcggcg tcgagcctgg cgccgttgtc ggagttccgg gaccggtgcc aattcatcca
     2101 tgttgcggga gaggccgacg ccgatgtgat ggaacaggtc tacgccgacg ccggatggaa
     2161 ccatttcgtg cggccctact gggaggacat cgccacgctt tacgccgccg cggattttgt
     2221 ggtatcacgt tccggcgcca acacggtgat ggaactggcg tatcttggaa aaaaggccct
     2281 gttggtgccc ttcccgttcg cgacgaacga ccaccagcgt ctgaacgccg cgtttttggc
     2341 caagtctggg cgggcggagg tggttttgga gaaggatttg tctcccggcc ggttccggga
     2401 gattttaaaa caggcttttg tctcaccgct ggaagcggag gccgctcccc ccaaggattt
     2461 ccctcgagcg acggcgtctc tggcggattt gttggaggat gttcgtttcg gtaaaactta
     2521 aacgtgggcc ggcaggagga cactcagcga tgaaaagaca acctaagcgt ggttccaaac
     2581 ccagcatatt catcagcaac gacgacggga tttatgggcc ggggctgaag cccttggtgc
     2641 gggccttgaa gtccctgggc cgggtgaccg tggtggtccc ggaactggaa cgttccgccg
     2701 gcagccacgc catcacgctg cacaaaccca tccgggtgca ccgcctggac cggtcgacgc
     2761 tcatcatgaa cggcaccccg gccgattgtg tgcggttcgg cgttctgtcc ctgctcaaag
     2821 aggacgtgga tgtggtggtt tccggcatca atcgtggtcc caacctcggg gcggacacca
     2881 tctattcggg caccgtcggg gcggccatgg aaggctgcat gatggggatc ccgtccctcg
     2941 ccgtttccct cgccgatgtg gggaggggga actatgaaac ggcggcgcgt ttcacccggg
     3001 ccctggtgaa ggttcttctc gagaggcccg tgccgaagga tatctgctac aacgtgaacg
     3061 tgcccgaccg tcctttcggc cgcatgcgcg gcgtgtcggt gacgcacttg gggaaacgga
     3121 tctacgggaa agacctggaa tcggggatcg atccgcgggg acaggcgtac tactggctgg
     3181 ccggagaaac accgacgggc atcgcggtgc cggggtcgga catcgccgcc attcagcagg
     3241 gaaaaatatc ggtgacgccg ctgcggctgg acatgacggc gacggaaatg attcccgtcc
     3301 tgcgccagtg gtcgtggcgt tactagggag ccgggcggga cggtaggccg ttcgaaacgt
     3361 ctcatgcccg ccgatgaccg gcgggccgtt ttttatggat aaattcaagg tcgtttccaa
     3421 attcaagccc gcgggggacc agcccgaggc catccgtcaa ctcgtcgagg gccttcgtca
     3481 aaagcagtgc catcaggtcc tgctgggggt gacggg
//
LOCUS       DAOTFD010000255         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20194_length_3514_cov_4.216537, whole genome shotgun sequence.
ACCESSION   DAOTFD010000255 DAOTFD010000000
VERSION     DAOTFD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20194_length_3514_cov_4.216537"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..398)
                     /locus_tag="P5079_10785"
     CDS             complement(36..398)
                     /locus_tag="P5079_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRY30504.1"
                     /translation="MRYVDGYVLPVPRKMVKAYLRMARMGERVWRKNGALDYMECVGD
                     DLHPKMGLSFPRLMRLRPGETAVFAFVVFKSRAHRDRVNARVMKDMMKMGGPKEMPFD
                     VKRMAYGGFKAAVGGRAA"
     gene            complement(449..880)
                     /locus_tag="P5079_10790"
     CDS             complement(449..880)
                     /locus_tag="P5079_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRY30505.1"
                     /translation="MNHERARTIALFLLRGVSGLLFLQAGGMKLFDWFGGIPPSFGGH
                     PALFSQVWIGGVLEFYGGAAILLGLFTRPVAFLLSGEMAVAYFQFHQPSGFWPIQNHG
                     EPAVLLCFIFLFFAAHGAGNWSLDALRRRWKENRSHGEPAS"
     gene            complement(906..1388)
                     /locus_tag="P5079_10795"
     CDS             complement(906..1388)
                     /locus_tag="P5079_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY30506.1"
                     /translation="MSNIKPVPEGYHTLTPYLTIKDAAQAIEFYKKAFGAKELYRLSD
                     PGGRIGHAELQIGDSRLMLSDEFPEMEGRGPRTLGGTPVMLHLYVEDSDELVRRAASA
                     GARVLSPVEDQFYGDRGGKVQDPYGHIWYISTHKEDLSAEEIDRRAREKFGFKHVQAA
                     "
     gene            complement(1559..2035)
                     /locus_tag="P5079_10800"
     CDS             complement(1559..2035)
                     /locus_tag="P5079_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY30507.1"
                     /translation="MSRRDEAPVISRSVPAEAGEILALQKRAFRTEADRYRCCDIQPL
                     KQTLEELRVEYKTKVVLKAVVGQTIVGSVRGSQTGDLCWVEKLMVHPDFRKRGLGTAL
                     MGELERCFPTAARFGLSTAEKSEDNVRLYEKRGYVVVDRVVGDKDIAMVTMEKRIS"
     gene            complement(2032..2370)
                     /locus_tag="P5079_10805"
     CDS             complement(2032..2370)
                     /locus_tag="P5079_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRY30508.1"
                     /translation="MASDEDFVKFIVDQIGRAGAVTYRKMFGEYALYCDGKVVALVCD
                     NRLFVKPTEGGRSFIGDVVEAPAYPGAKKSFLIEDKFEDRDWITRLIEITAREVPSLP
                     PKKKKRGTPR"
     gene            complement(2404..3108)
                     /locus_tag="P5079_10810"
     CDS             complement(2404..3108)
                     /locus_tag="P5079_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRY30509.1"
                     /translation="MNTVIESVRKELRRNAVDAVRDGVRRFFKERVKLHGVKTPVVRA
                     LAARTARQLRGVEKERLFALCEKLLRSNYMEEALIAFEWADGRRKEFVRGDFKRFERW
                     VKTYVDNWAKCDTFCNHTVGSFVERYPEFIGKLREWTRSPNRWVRRAAAVTLILPARH
                     GKFLSESLEIAGRLLPDEDDLVRKGTGWLLKAAAEKHAREVFNYVLRNKKKMPRTVLR
                     YAIEKMPAALRRRAMA"
BASE COUNT          602 a         1165 c         1019 g          728 t
ORIGIN      
        1 ttttcccgcc gccggagaac gccgagtccc cggttttagg cggcgcggcc gccgaccgcg
       61 gccttgaaac ccccgtaggc catgcgcttg acgtcgaagg gcatctcctt cggtcccccc
      121 attttcatca tgtccttcat cacgcgggcg ttgacgcggt ctcggtgcgc tcgtgatttg
      181 aaaacgacga acgcgaacac cgcggtctcg ccgggccgaa gcctcatcag gcgggggaac
      241 gaaagcccca ttttcggatg aaggtcgtcc ccgacgcatt ccatgtagtc gagggcgccg
      301 tttttgcgcc acaccctctc tcccatccgg gccatgcgga gataggcttt gaccatcttc
      361 ctgggcaccg gcaggacata gccatccacg tatctcatat gttgcctcct cggaccgatc
      421 cggggggggc cgccgcggga cggactgtct acgaggcggg ttccccgtgg gaccggtttt
      481 ccttccatcg ccgccggaga gcgtcgagac tccaattccc ggcgccgtgg gcggcgaaaa
      541 acaggaaaat gaaacaaaga aggacggcgg gctcgccgtg gttctggatg ggccagaatc
      601 cgctcggctg gtggaactgg aagtaggcga ccgccatctc ccccgagaga aggaaggcca
      661 cgggtcgcgt gaagagcccc aacaggatgg ccgccccgcc gtaaaactca agcacgccgc
      721 cgatccagac ctgcgagaaa agcgccgggt ggccgccgaa cgagggcggg atccctccga
      781 accagtcgaa gagtttcatc ccgcccgcct ggaggaacaa cagccccgaa acacccctca
      841 acaggaacag ggcgatggtc cgagcgcgtt cgtgattcat gcgtccctcc tcgcaccccg
      901 cggcttcagg ccgcctgaac gtgcttgaag ccgaatttct cacgggcgcg ccggtcgatc
      961 tcctccgccg agagatcctc cttgtgcgtg gagatgtacc agatgtgtcc gtacgggtcc
     1021 tgcactttgc cgccgcggtc gccgtagaac tggtcctcca cgggggaaag gacccgggcg
     1081 ccggcggacg ccgcccgacg gaccagctcg tccgaatcct ccacgtagag gtgcagcatc
     1141 acgggcgtgc cgcccagcgt gcgcggacct cggccttcca tttccgggaa ctcgtcgctc
     1201 agcatgagcc gcgaatcgcc gatttgaagt tccgcgtgcc cgatccgccc cccggggtca
     1261 ctgagccggt agagctcctt tgcgccgaac gccttcttgt agaactcgat cgcctgggcg
     1321 gcgtctttga tggtcaaata aggcgtgagc gtatgatagc cttcgggaac aggtttaatg
     1381 ttggacatac gtacctcctt cttgtgtaga tcacgtcatt aacacgcggg ggtcccgaaa
     1441 ggttccgaac tccgttccct tgggggggcg gatttttcag gaccgtttgg cgaaaaacgt
     1501 gtgtcttttt gagtgtggga gggggacgac ggcgaaaagg cggcggggcg atggggctct
     1561 aggagatccg tttctccatc gtgaccatgg cgatgtcctt gtcgccgacg acgcggtcga
     1621 cgacgacata gccccgtttt tcgtaaaggc ggacgttgtc ttcgcttttt tccgcggtgc
     1681 tcagaccgaa ccgcgcggcc gtgggaaaac accgctccaa ttcccccatc agcgccgttc
     1741 cgaggcccct cttgcggaaa tcggggtgca ccatgagttt ttccacccaa cagagatccc
     1801 cggtttggga cccccgcacg gaaccgacga tcgtttgtcc gacgaccgct tttaaaacca
     1861 ccttggtctt atattcgacc cgcagttcct ccagggtctg tttcagcggc tggatgtcgc
     1921 aacaacggta ccggtcggcc tccgttcgga acgcccgttt ttggagtgcg aggatctccc
     1981 cggcctcggc gggcacggaa cgtgaaatga cgggggcctc gtcccggcgg ctcatcgggg
     2041 cgttcctctc ttctttttct tcggggggag cgaggggacc tcccgcgccg tgatctcgat
     2101 caatcgggtg atccaatccc ggtcttcgaa cttatcctcg atgagaaagc tctttttggc
     2161 gccggggtag gccggggcct ccaccacgtc gccgatgaac gatcttcccc cctcggtcgg
     2221 tttcacgaac agccggttgt cgcaaacgag ggcgaccacc ttgccatcgc aatagagcgc
     2281 gtattcgccg aacatcttcc gataggtgac ggcgcccgcc cggccgattt gatcgacgat
     2341 gaatttgacg aaatcctcat ccgaagccat ttttctcccc tccccgccgt ccttaaaaaa
     2401 tccctaggcc atggcccggc gccggagagc ggcgggcatc ttttcgatgg cgtagcgcag
     2461 gaccgtccgc ggcatctttt tcttgttccg cagaacgtag ttgaaaacct cccgcgcgtg
     2521 tttctccgcg gcggctttca gcagccagcc ggtgcccttc cgcaccaggt cgtcctcgtc
     2581 cggcagaagg cggccggcga tttccaacga ctcgctcagg aatttcccat gccgggccgg
     2641 caggatgagc gtcaccgcgg ccgcgcgccg cacccagcgg ttgggggagc gggtccattc
     2701 ccgaagtttc ccaatgaact ccggatacct ctccacgaaa gaccccacgg tgtggttgca
     2761 gaaagtgtcg catttagccc agttgtccac gtaggtcttg acccagcgct cgaaccgttt
     2821 gaaatcgccg cggacgaatt ctttccgccg gccgtcggcc cattcgaaag cgatgagcgc
     2881 ctcctccatg taattcgacc ggaggagctt ttcgcagagc gcgaagagcc gctctttttc
     2941 cacgccgcgc agttgccggg cggtccgggc ggccagggcg cgcaccacag gggtcttgac
     3001 cccgtggagt ttcacgcgtt ccttgaaaaa acgccggacg ccgtcccgga ccgcgtccac
     3061 ggcgttcctc ctcagttctt ttctgacgga ctcgatgacg gtgttcatgg gaacgggaac
     3121 ccgaccgtcg cttcgcgcgg attccgacgc ccctcgttgc ccatccattt cctgtccccg
     3181 cctctcggca tttccaggcc cccgtctttc ctgtcgtttt tcccgccggg ccgtccgtcc
     3241 cattttattc cttcccgcat ccgcccgtcc ataaatttct tatcccccca gaaaactccg
     3301 gcaaggaatc ttgtgcatcg ccgtggcatt gtatacactc cccccgtgct gttccttcgc
     3361 ggcctgtttc cgttcctccg gcgccacaaa aacgccgccg tcttcgtggc cggctttttg
     3421 ttcgacgcct tcaccacgcg gatcgactcg tggatcgatc tgtccctcca attcctctac
     3481 ctggcggtgc tgacggcgct tctcttcttc caat
//
LOCUS       DAOTFD010000256         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20317_length_3500_cov_3.826415, whole genome shotgun sequence.
ACCESSION   DAOTFD010000256 DAOTFD010000000
VERSION     DAOTFD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20317_length_3500_cov_3.826415"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="P5079_10815"
     CDS             complement(<1..847)
                     /locus_tag="P5079_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30510.1"
                     /translation="MKTRSASSFRRLRFFLTAFFALGLLPLTSFGFEQLKLAMERALE
                     ERIQSALSKLIDPDQFLVIVKLEPFTADELQSKSKGGTAANLPQYVLPGIPERQSIDN
                     LNSGKSEKQDILWQSRPFIKRLHITLFLDKYIRDDLAQQIEILVKQLVDFNPSRQDEL
                     KLSRIVVRRASQLREAAGDAKTPMQQVKFTIEQIKERKDFYWILLGVAITGSIIVFLF
                     GPMLYFLRRFPKILQQAFLPEGGGYSGAGSSGGGGAGSGGGGGTTKLEVDNALPLALN
                     LDAKAA"
     gene            complement(884..1822)
                     /locus_tag="P5079_10820"
     CDS             complement(884..1822)
                     /locus_tag="P5079_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30511.1"
                     /translation="MTCPVARRLLSVSMFFCLALALPGPLRAWQWSDGQNTVLPSLQE
                     EFDQEHNAYRSQVEKGALVGQERLIALDRLISHYKPLGINTVVLESERDRLTVQLEAD
                     RQKSQSAQKISSELYQLALQKTGEGRFRDALSAIQKAERLVPEDAAFAEMRRRLDSVT
                     AIVSQAVQNTKVDGLVRKGVVRYIENDGTRALNAVRFAQQLSPSEPSLDRLGKLIEKE
                     YPGLEPPSIPKGKTLVDYKLQLALEYVYDQQFLKAINECNQVLDLEPDNVLALTRMGS
                     AYYAMGQKDEAKTLWVRALKLEPNNQVLKQFLREKF"
     gene            complement(1852..2229)
                     /locus_tag="P5079_10825"
     CDS             complement(1852..2229)
                     /locus_tag="P5079_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30512.1"
                     /translation="MAPERILVIEDDPDVRDTVCGALESVFPDASVTALADGREFLDT
                     FSAAAVWDLIILDLMLPGISGVEICRRVHADAAGRHIPILAMTGYDTPEMEKRVKEAG
                     AAAYLAKPFEMKDFVRIVKRLVG"
     gene            complement(2201..>3500)
                     /locus_tag="P5079_10830"
     CDS             complement(2201..>3500)
                     /locus_tag="P5079_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY30513.1"
                     /translation="NQIQAASPADEDIQRLMPRYDDVLKSAQNVIGLYRVSKDRAFNK
                     ATDDLLPKVRRFFDDVNQTEARIAAEVKNAQTRVKEMVFLGKRLVLGGGLLGACVGFL
                     FALFTFRSLTKSLRALQQGTAAFGSGQWDYRIDLPVQNELGELARSFNEMASNIKHLE
                     TQTVHMHRMSAVGQLAGGVAHEINNPLTGVLGQAQLLLEKLPSDDPRRAHLEKIERAA
                     VRCKAIVRSLLDFSRQKEAQFTAMDINEVLEATLEFCEADLQSNHITVLKNFSATVPK
                     ITGSSSQLQQVFLNIITNAIQAMPKGGTLSVAAKPTRFTVGPSPDAPAVEGIEISFTD
                     TGVGVETDHLRHIFEPFFTTKEIGKGTGLGLSVSLGIVKNHGGDIRAESRGKGAGSVF
                     SVLLPLVSMAPSSARSDSSPLPVPVGGERARFGPGADTRY"
BASE COUNT          633 a         1026 c         1140 g          701 t
ORIGIN      
        1 ccgcggcctt ggcgtccagg ttgagagcca acggaagggc gttgtccacc tccagcttgg
       61 tggtcccacc gccgcccccg cttccggccc cccccccgcc ggaacttccg gcgcccgaat
      121 acccgccgcc ctctggtaga aaagcctgct ggaggatctt cggaaaacga cgcaggaagt
      181 aaagcatggg gccgaaaagg aagacgatga tggagccggt aatggccacg ccgagaagta
      241 tccaataaaa atcttttcgt tccttgatct gttcgatggt gaacttcacc tgttgcatgg
      301 gggttttggc gtcgcccgcc gcctcgcgca gctgcgacgc ccggcggacg acgatgcggg
      361 acagtttgag ctcgtcctgg cgggaaggat tgaagtccac cagttgtttg acgaggatct
      421 cgatctgctg ggcgaggtcg tcccggatgt atttatcgag gaagagtgtg atgtgaagcc
      481 gtttgatgaa cggccggctc tgccagagga tgtcctgttt ttccgatttg ccggaattca
      541 ggttgtcgat ggactgcctt tccggaatgc ccggaagcac atactgaggg aggttggcgg
      601 cggtcccccc cttggatttg gactgaagct cgtcggcggt gaagggctcg agcttgacga
      661 tgacgaggaa ctggtccggg tcgatcaact tcgaaagggc ggactggatc cgttcttcca
      721 aggcccgttc catggccaat ttcagttgtt cgaaaccgaa ggaggtcagc ggcaggaggc
      781 cgagggcgaa gaacgccgtc aggaaaaacc ggaggcggcg aaaagaagag gcggaacgcg
      841 ttttcatgac cggaaggatt gtattaattt ttacaaggcc gtatcaaaat ttttcgcgaa
      901 ggaactgttt caagacctga ttgttgggtt ccagtttgag cgcccgcacc cagagcgtct
      961 tggcttcgtc tttctggccc atggcgtaat aggcggaccc catgcgcgtg agggcgagga
     1021 cgttgtccgg ttcgaggtcc aagacctggt tgcattcgtt gatggctttc aggaactgtt
     1081 ggtcgtagac gtattccagg gcgagttgaa gcttatagtc caccagggtc ttgcccttgg
     1141 ggatggacgg aggctccagg ccggggtatt ccttttcgat gagtttgccc aaacggtcca
     1201 gcgagggctc ggacggagag agctgctggg cgaaacgcac ggcgttgagc gcgcgggtcc
     1261 cgtcgttctc gatgtaccgg accacgcctt tgcgcaccaa cccgtccacc ttggtgttct
     1321 gaaccgcctg ggacacgatg gcggtgacgc tgtcgagccg ccggcgcatc tccgcgaaag
     1381 cggcgtcctc ggggacgagg cgctcggctt tttggatggc ggagagggcg tcacggaaac
     1441 gcccttcccc cgttttttga agcgccagct gatacagctc ggaggaaatt ttctgggcgc
     1501 tctgggattt ctgccggtcg gcctccaact ggacggtcag ccggtcgcgc tccgactcca
     1561 ggaccacggt attgatgccg agcggtttat agtgggagat cagccggtcc agcgcgatca
     1621 gccgttcctg gccgaccaag gcgccttttt caacctggct tcggtaggcg ttgtgctcct
     1681 ggtcgaattc ctcctgcagg ctgggcaaca cggtgttttg cccgtcggac cactgccatg
     1741 cccgaagcgg gccggggagc gccaacgcga ggcagaaaaa catcgacacc gagagcaacc
     1801 gacgggcgac gggacaggtc atggggttcc cttcctcttt tttttggaaa ctcaaccgac
     1861 gagccgcttc acgatccgga cgaaatcctt catctcgaac ggcttggcca gataggcggc
     1921 cgcgccggcc tctttgaccc gcttttccat ctccggcgtg tcgtaccccg tcatggccag
     1981 gatggggata tgccgtccgg cggcgtcggc gtggacgcgc cggcagatct cgacgccgga
     2041 aatccccggc agcatcaggt ccaagatgat caaatcccag acggcggccg cgctgaacgt
     2101 gtccaaaaac tcccgtccgt cggccagcgc ggtgacggac gcgtcgggga agaccgattc
     2161 cagggcgccg cagaccgtgt cgcggacgtc cggatcgtcc tcaataacga gtatccgctc
     2221 cggggccaaa acgagctctt tccccgccga ccgggaccgg caacggcgat gaatcggatc
     2281 gtgcggaaga cggcgccatc gacaccagcg gcaaaagcac ggagaacacg gaccccgcgc
     2341 ccttcccgcg gctctccgcg cgaatgtccc ccccgtggtt cttcacgatc cccaaggaga
     2401 ccgaaagccc caacccggtg cctttgccga tctccttggt ggtgaagaac ggttcgaaga
     2461 tatgccgcag atggtccgtc tcgacgccga cgccggtgtc ggtgaaggag atctcgatgc
     2521 cctccaccgc cggggcgtcc ggggacgggc cgacggtgaa acgcgtcggc ttggcggcca
     2581 ccgacagggt gccgcccttg ggcatggcct ggatggcgtt ggtgatgatg ttgagaaaga
     2641 cctgttggag ctgggaactg ctgccggtga tcttgggcac ggtggcgctg aagtttttca
     2701 gaacggtgat gtggttgctt tgcaagtccg cctcgcagaa ctccagggtg gcttccagca
     2761 cctcgttgat gtccatggcg gtgaactgcg cctctttctg cctggaaaaa tccaacaggg
     2821 agcggacgat ggccttgcag cggacggcgg cgcgttcgat cttttccaag tgggcgcggc
     2881 gcgggtcatc cgacggcagt ttctccagga gcagctgcgc ctgtcccagc acgccggtga
     2941 gcgggttgtt gatctcgtgg gccaccccgc cggccagctg ccccacggcg ctcatgcggt
     3001 gcatgtggac ggtctgggtc tccaggtgtt tgatgttgct ggccatttcg ttgaacgagc
     3061 gggccagctc ccccagttcg ttctgcaccg gcaggtcgat gcggtaatcc cactgaccgc
     3121 tgccgaaagc cgccgtgccc tgctgcaagg cgcgcaggga ttttgtcaaa gagcggaagg
     3181 tgaacagggc gaaaaggaaa ccgacgcacg cgccgaggag cccgcctccc aggaccagcc
     3241 gtttccccag gaacaccatt tccttcacgc gagtctgggc gttcttcacc tccgcggcga
     3301 tgcgggcctc cgtttggttc acgtcgtcga agaaccggcg caccttgggg aggaggtcgt
     3361 cggtggcctt gttgaaggcc ctgtctttgg agacgcgata gaggccgatg acgttctggg
     3421 ccgacttcag cacgtcgtcg tagcgcggca tcagtctttg gatgtcttcg tcggcgggtg
     3481 aggccgcctg gatctggttc
//
LOCUS       DAOTFD010000257         3486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20436_length_3486_cov_3.695133, whole genome shotgun sequence.
ACCESSION   DAOTFD010000257 DAOTFD010000000
VERSION     DAOTFD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20436_length_3486_cov_3.695133"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3486
                     /locus_tag="P5079_10835"
     CDS             <1..>3486
                     /locus_tag="P5079_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HRY30514.1"
                     /translation="LKDTKVTMPDGSHKSLYDLLGGDSGGLMKLMRDGAIYWESGVLV
                     LGTDADGTSIVQVNISFRDQAGRVLGSFGVSYDNEKHGFQRAFSFNFSNVAVRAAVQQ
                     FLMQLFGVSTLAALEQKLKQEGIQSLSEIFVGGGGQRQGGGNGNRQEGEGVGSRPTVA
                     RANRSLLDRIRERVAARREMMSSRQFMRMRDLDGPMDEWQDEPMDEEAAVSPADEPEG
                     APEEQEEEEDRGGGRRSLADIFGPQFMSMLRGGHINWSQGSLTLGVDAEGNSFLGVSV
                     SFVTANNRPLGQINVAWNNKQHGFDVVMNLEFRNPEVRRAVQNLLILLLNVTDQNHDG
                     SRVDDLERAIRGVGSDALANIQISIEKPDGSIEKVSLADMFGDEFVSMLSGARVNWNL
                     GSVSFGRSANGDSIFQVSLPLVDASGRKLGTFSIAWDATARGFSRVFTLNLSHPAVRA
                     AVIQFLARLLNVTDADNDGSLLDELETKLKELGPGALDKIKIRVPDPDHPGQTKEVSL
                     GSFFGEEFLQMLSAGHIDWSSGTLSFGRSAKNELIMGISAAFRDADNKIIGHFNIAYD
                     SSKQGIGYFLQLNFDNPAVRAAVQNFLINLLGVQDADNDGSRLDDLEAKLKDLGYGGL
                     QSMLVDMPGGGKKSLGELLGADFFNFLNNEGRIDWNSGSVTIGTDASGKKVFQATFSF
                     RDSLGREIGTYAVTYDDAEHGLSYTLALNMKHDKVWAAVNRMKGMINASAADALARIP
                     VQLQAPGEQPGTTTPPTTPHPPQGYCFAAGTPVLTRSGLKPIETIQVGEEVLSYNESK
                     TVAEWQKVVATPRSERDGLVELRLNGEILRCSNNHRFYTVNRGWVQADRLVPGDNLMD
                     KEGRAVVVTEAAVPLDGKAPVFNLTVANNRNYFAGRGSLLVHNRKMMDPDAGGDGGYP
                     GEDGGEGEEPVSAPASTEPPAMNLGELLGEDFLNAIAGASISREDSLLTLGTGADGKR
                     IISAQFALFSGGRKLGTYTIGYDPDKKGLSVGYSLEMSHPLVQAAVQRFLMVLLGVDP
                     AASNALQLLEAKLKEVTADTLTETVVTIGGQRVTLGDLLGDDFVDALENGFIDWNDGT
                     MSFGFGADGKAYFQVSIAYRDQNGNVLGHMNIVYDSAKQGFSTTLSFNMENLFVRNAV
                     QNLLRGLLQLPPTAS"
BASE COUNT          700 a          905 c         1158 g          723 t
ORIGIN      
        1 gctgaaagac accaaggtga ccatgccgga cggttcgcac aagagcctct acgatcttct
       61 cgggggcgat tccggcggtc tgatgaaatt gatgagggac ggggccatct attgggagag
      121 cggggtgctg gttcttggga ccgatgcgga cggcacatcc atcgttcagg tgaatatttc
      181 gttccgggat caagccgggc gggtactggg ttctttcggt gtttcttacg acaatgaaaa
      241 acatgggttc caaagagcct tcagcttcaa tttctccaat gtcgctgttc gggcggccgt
      301 ccagcaattt ctgatgcagc ttttcggggt ttcgaccctg gcggcgcttg agcagaaatt
      361 gaaacaagag ggcatccaaa gcctctctga aatctttgtg ggcggcggcg ggcagcgcca
      421 aggcggtggg aacgggaacc ggcaggaagg ggagggcgtt gggtcccggc cgacggttgc
      481 ccgcgcaaat cggtccctgc tcgatcggat tcgtgagcga gtggccgccc ggcgggaaat
      541 gatgtcgtcc cgccaattca tgcggatgcg ggacttggat gggccgatgg acgaatggca
      601 ggacgaaccg atggacgagg aggctgcggt ttcccccgcc gatgagccgg agggggctcc
      661 cgaagaacag gaggaagagg aggatcgggg aggtggtcgc cggtctttgg cggatatctt
      721 tgggcctcag tttatgtcca tgctccgggg aggacatatt aactggagcc agggaagttt
      781 gacgttgggg gtggatgccg aagggaacag cttcctgggt gtttccgtca gtttcgtgac
      841 ggccaataac cgtcccttgg gacagatcaa cgtggcgtgg aacaacaagc aacacgggtt
      901 tgacgtggtt atgaatttgg aatttcgcaa ccccgaggtt cggagggcgg tgcagaacct
      961 gttgatcctc ctcttgaacg tcaccgatca aaaccatgac ggcagtcggg tggacgactt
     1021 ggagagggcc atccgcggcg tgggaagcga cgcgctggcg aacatccaga tctcgattga
     1081 aaaaccggac ggttcgatcg aaaaggtgtc gctggcggac atgttcgggg acgaattcgt
     1141 ctcgatgctg tccggggcgc gcgtcaattg gaacctcggt tccgtttcct tcggccgctc
     1201 ggccaacggg gacagcatct tccaggtctc gttgcctctg gtggacgcct ccggccggaa
     1261 actggggacg ttttccattg cttgggacgc gacggcgcgc ggtttttcca gggtgttcac
     1321 gctgaacctc tctcatccag cggttcgcgc cgcggtcatc cagttcctcg cgcggcttct
     1381 gaacgtgacc gatgcggaca acgacggttc gcttctggat gagcttgaga ccaaactcaa
     1441 ggaattgggt cccggcgccc tggataagat caagattcgc gttccggatc cggaccatcc
     1501 gggtcagacg aaagaggtca gtctggggtc gttcttcgga gaggaattcc tgcagatgtt
     1561 gtcggcggga catatcgact ggagcagcgg aaccttgagt ttcggccgca gcgccaagaa
     1621 cgagttgatc atggggattt ccgccgcgtt ccgggatgcg gacaacaaga tcatcggcca
     1681 cttcaacatc gcctacgatt cctccaagca agggatcggt tatttccttc aattgaattt
     1741 cgacaacccg gcggtccggg cggcggttca gaatttcctc atcaatcttt tgggggttca
     1801 ggacgccgac aacgacggtt cgcggctgga tgatttggag gccaagctga aggatctggg
     1861 ctacggcggc ttgcaatcca tgttggtgga tatgccgggg ggcggcaaga aaagtttggg
     1921 agaattgctg ggggcggatt tcttcaattt tctcaacaac gaggggcgta tcgattggaa
     1981 ttccgggtcc gtcaccatcg gaacggacgc cagcgggaaa aaggtttttc aggccacgtt
     2041 ctccttccgg gactctttgg gacgggaaat cggcacatac gccgtcacct acgacgacgc
     2101 cgagcatggc ctaagctata ccttggcgtt gaatatgaag cacgacaagg tctgggcggc
     2161 tgttaaccgc atgaagggga tgatcaacgc gtcggccgcc gacgccttgg cccgtatccc
     2221 cgtccagctc caggctccgg gggaacagcc ggggaccacc acgccgccga caacgcctca
     2281 tccgccgcag gggtattgtt tcgccgccgg gacccccgtc ctgacgcgtt ccggattgaa
     2341 acccatcgaa acgatccagg tgggggaaga ggtcctttcc tataacgaat cgaaaacggt
     2401 ggcggaatgg cagaaggttg tggccacgcc acgaagtgag cgggatggct tggtcgagtt
     2461 gcgcttgaac ggggagatcc tgcgctgctc caataaccac cgtttttaca cggtcaaccg
     2521 cggttgggtt caggcggatc ggcttgtgcc cggggacaat ctgatggaca aagaaggccg
     2581 ggccgtcgtg gtgaccgagg ccgccgtccc gttggacggg aaagcgccgg tattcaacct
     2641 gaccgtcgcc aacaaccgga actattttgc gggacggggt tcgcttttgg ttcacaacag
     2701 gaagatgatg gatccggacg ccggagggga cggagggtat ccgggagaag acggagggga
     2761 gggggaggag ccggtttccg cgcccgcctc cacggaaccg cccgcgatga atttgggcga
     2821 gttgctgggg gaggatttcc tcaacgccat tgccggagcc agcatcagtc gggaagattc
     2881 cctgctcacc ctggggaccg gggccgatgg gaagaggatt atcagcgccc agttcgcgct
     2941 tttctccggg ggacgaaagc tgggcaccta caccatcggt tatgatcccg acaagaaggg
     3001 gctttcggtg ggctattcgc tggaaatgtc ccacccgctc gtgcaggccg cggtgcagcg
     3061 gttcctgatg gtgttgttgg gggtggaccc ggcggcctcg aacgcgcttc agctcttgga
     3121 ggccaaattg aaggaggtga cggcggacac cctgacggag accgtcgtga ccatcggggg
     3181 gcagagggtg accttgggcg acctgttggg ggatgatttc gtcgacgcgt tggaaaatgg
     3241 ttttatcgat tggaacgacg gcacgatgag tttcggtttt ggcgccgatg gaaaagcgta
     3301 tttccaggtg tccatcgcct atcgcgatca gaacggcaat gtgctgggcc atatgaacat
     3361 cgtctatgat tcggcgaaac aagggttcag caccacgttg agtttcaaca tggagaattt
     3421 gttcgtccgt aacgcggtcc agaacctcct ccggggactc ttgcagcttc ctccgacggc
     3481 gtcttt
//
LOCUS       DAOTFD010000258         3472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20561_length_3472_cov_4.505999, whole genome shotgun sequence.
ACCESSION   DAOTFD010000258 DAOTFD010000000
VERSION     DAOTFD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20561_length_3472_cov_4.505999"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="P5079_10840"
     CDS             complement(<1..364)
                     /locus_tag="P5079_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30515.1"
                     /translation="MPHRFSRCFAAVCSCLLLSLFINAPSGLRAANPLYLELSAHGDR
                     IDLGLAAFSTSRTLVEEAAIAREMQEVTRQDLIFPRIFNLIEGGVPPVRKKVLLDSWS
                     RLGADVVVTGYVDTGWFGR"
     gene            complement(382..>641)
                     /locus_tag="P5079_10845"
     CDS             complement(382..>641)
                     /locus_tag="P5079_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30516.1"
                     /translation="LFAREKWDDAVAAYDKVLVKYSTSSVVPSAYLQKGLTLEKAGKP
                     AEAVPVYEAVARKYPHRQEASSAQLRLEALKNPPVPAEPPR"
     assembly_gap    642..651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<652..1157)
                     /locus_tag="P5079_10850"
     CDS             complement(<652..1157)
                     /locus_tag="P5079_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30517.1"
                     /translation="MGHRRRAPLLMLLAGALLCLPGCLATRQEIEDLRADIIRLQNTL
                     SKAQTEAQSSLQGNQADLMSQMDTLGRKLETLSAHLDESESRMALMSSRMDDLDMNLS
                     SRLDLLSEMLSGSKLGVSPAPSTLFKLAYTDFSRRRYEQALKGFQTYLEKYGETEKAA
                     EAQYYTGE"
     gene            complement(1188..1748)
                     /locus_tag="P5079_10855"
     CDS             complement(1188..1748)
                     /locus_tag="P5079_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY30518.1"
                     /translation="MQTNAIFSKIGLFLLASAVLTGCPPKKKPIVPVEPPVAEAPVEE
                     PGIDVGEEWVSAPNLPLIYFETNKSDLTADARAVLKKNAPVLKAILAEASGVQVRVEG
                     HCDERNTLEYNLALGERRAAAVKDYYVSLGVAKTALSTVSFGEERPVCSLSEEGCWSK
                     NRRAATVLKSSSGPIRIPFSKLPAAE"
     gene            1945..2673
                     /locus_tag="P5079_10860"
     CDS             1945..2673
                     /locus_tag="P5079_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRY30519.1"
                     /translation="MLVDPEKISQNCAAILERLTQAAKRSGRSLADITLVAVTKNVPV
                     ESLGKAWAFGLRHFGENRVQEAERKKSDFAARFGDTPGLTWHLLGHLQSNKAKKAAAL
                     FDRFQSLDSFPLAERLNKEGLSLGRPLRCLVEVKISDEPTKQGVAPQDVDDFLARLAP
                     LEFIRVDGLMTIAPYLDDPRAGRPYFQRMRSLFDRNRPRFKTEAPVLSMGMSHDFETA
                     VEEGATMVRIGTAIFGERPRPEKA"
     gene            2670..>3472
                     /gene="proC"
                     /locus_tag="P5079_10865"
     CDS             2670..>3472
                     /gene="proC"
                     /locus_tag="P5079_10865"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRY30520.1"
                     /translation="MNLKKIRLALIGGGNMGAALAAGLVTSKKMRPAQIRVVDVRPEI
                     LRSLKKRYGVSVGSDNAAAVRGSDVVLLCVKPQQMASVLAGLLGAVTPKQLVISIAAG
                     IKTDFIESELGQHVPVIRVMPNTPALLRAGALVYCLGQHATKTHEALAKQILSSVGSV
                     WKTGELSMDAVTALSGSGPAYVFYLAECLAKAGAELGLEPGFAEALARQTVFGAGRML
                     HEDKETPARLRERVTSPGGTTEAALRVMAESGFEGILKRALAQARRRPGG"
BASE COUNT          638 a         1099 c         1096 g          629 t
ORIGIN      
        1 accgaccgaa ccagccggtg tccacgtatc cggtgaccac cacgtcggcc ccgagacgcg
       61 accaggaatc cagaagcacc tttttcctca ccggcggtac gccgccctcg atgaggttga
      121 agatgcgcgg gaagatcagg tcctgccgcg tcacctcctg catctcgcgg gcgatggccg
      181 cctcctccac cagcgtccgg ctggtgctga aagcggccag gcccaggtcg atgcggtcgc
      241 cgtgagcgga cagttcgaga taaagcgggt tggcggcgcg gaggcccgag ggggcgttga
      301 taaaaagact gaggaggagg caggaacaaa cggcggcgaa acatcttgaa aaacgatgcg
      361 gcatggtcga agaatcgtcg ttcagcgggg cggctccgcg gggaccggcg gattcttcaa
      421 agcctccaaa cgcaactggg ccgaggaggc ctcctggcgg tgcgggtatt tgcgcgccac
      481 cgcctcatac accggcacgg cctcggccgg tttccccgcc ttctccaggg tcagcccttt
      541 ctggagatac gccgaaggca ccacggaact cgtcgaatat ttcaccagca ccttgtcgta
      601 ggcggcgacg gcgtcgtccc acttctcacg ggcgaacagg cnnnnnnnnn ncattccccc
      661 gtataatact gcgcctcggc ggctttttcc gtctccccgt atttctccag ataggtctga
      721 aaccccttca gcgcctgctc gtaccgccga cgggaaaaat ccgtgtacgc caacttgaac
      781 aacgtgctgg gcgcgggaga aacgccgagc ttggatccgg aaagcatttc ggagaggagg
      841 tcgagccggc tggataaatt catgtccagg tcgtccatgc gggaggacat cagcgccatg
      901 cggctttcgc tttcgtccag gtgggcggag agggtctcca gtttccggcc cagggtgtcc
      961 atctgcgaca tcaggtcggc ttggttcccc tgaagggaag actgcgcctc ggtctgggct
     1021 ttggagaggg tgttctgaag gcggatgatg tcggcccgga ggtcttctat ctcctgccgg
     1081 gtggccaggc agccgggcag acacaacaac gcgccggcca acaacatcag caacggcgcg
     1141 cggcgtcggt ggcccattcg ttccgttccc tttcccgccg acgcgcctta ttccgcggcg
     1201 gggagcttgc tgaaggggat gcggatggga ccggaagaag atttcaggac ggtggcggcg
     1261 cggcgatttt tcgaccagca gccctcctcg ctgagggagc aaaccggccg ttcttcgcca
     1321 aaagaaaccg tggagagcgc cgtcttggcc acgcccaggg agacgtaata gtccttcacc
     1381 gcggcggcgc ggcgctcgcc cagcgccaga ttgtattcca gcgtgttgcg ctcgtcgcag
     1441 tggccttcca cccggacctg aacgccggag gcctcggcca aaatggcctt caaaacgggg
     1501 gcgttcttct tgagcacggc gcgggcgtcg gccgtcaggt ccgatttgtt cgtttcaaag
     1561 taaatgagcg gcaggttggg cgccgacacc cactcctcgc cgacgtcgat ccccggttcc
     1621 tccaccggcg cctcggcgac gggcggttcc accggcacga tgggtttttt cttgggcggg
     1681 caacccgtca aaacggccga cgccaggagg aacaggccta ttttcgagaa aatcgcgttt
     1741 gtctgcatgg caaaactcct tttttatgat cgttcagccc ttaattttag tatatgcctc
     1801 cgccgcccgc aagccttgcg acgagaggag gattttcgta taatacctat aaagaagtgg
     1861 gcggcgtttt accccggatt cccatcccgc tccgcgctcc atgggccgtt ggccggcagt
     1921 ttccacgcat taaaaacccg ttttatgctg gtggatcccg aaaagatttc tcaaaactgt
     1981 gcggctattc tggaaaggct cacccaggcc gcgaaacgat ccggccggtc tttggcagac
     2041 atcaccctcg tggccgtgac caaaaacgtg cccgtcgagt ctctcggcaa ggcctgggcg
     2101 ttcggcctcc gccatttcgg cgaaaaccgg gtgcaggaag cggaacggaa aaaatcggat
     2161 ttcgcggcgc gttttggcga cacccccggc ctcacctggc acctgctcgg ccacctgcaa
     2221 agcaacaagg ccaaaaaagc ggcggcgctg ttcgaccggt tccagtccct ggacagcttt
     2281 ccgctggcgg aacggctgaa caaggaaggc ctctccctgg gacggcccct ccggtgcttg
     2341 gtggaggtga agatctccga cgaacccacc aaacaggggg tggcgccgca agacgtggac
     2401 gattttttgg cgcggttggc ccctctcgaa ttcatccggg tggacggcct catgaccatc
     2461 gcgccgtatc tggacgatcc ccgggccggg cggccgtatt ttcaacggat gcggtccctt
     2521 ttcgaccgga accgcccacg gttcaagacc gaggcccccg tgttgtccat gggcatgtcc
     2581 cacgattttg agacggcggt ggaggaaggc gccaccatgg tgcgcatcgg aacggcgatt
     2641 ttcggcgaac gtccccgccc ggagaaggca tgaacctaaa aaagatccgc ctcgccctca
     2701 tcggcggcgg caacatgggc gccgcgctgg cggcggggct ggtgacctcg aaaaaaatga
     2761 ggcccgccca gatccgcgtc gtcgacgtgc ggcccgaaat tttgcgttct ttgaaaaaga
     2821 ggtatggcgt gtcggtcggc tcggacaacg cggccgcggt ccgcggaagc gacgtcgtcc
     2881 tcctctgcgt gaaaccgcag cagatggcgt ccgtcctggc cggccttctc ggcgccgtca
     2941 cgccgaaaca gctcgtcatc tccatcgccg cggggataaa gacggatttc atcgagtccg
     3001 agctgggcca acacgtgccc gtcatccgcg tgatgcccaa caccccggcg ctcttgcgcg
     3061 ccggcgccct ggtctattgc ctcggccagc acgccacgaa aacgcacgag gccctggcca
     3121 aacagatttt atcgtcggtg ggttccgtgt ggaaaaccgg cgagctctcg atggacgcgg
     3181 tcacggcgtt gtccggctcc ggcccggcct acgtgttcta cctggccgaa tgtctggcca
     3241 aagccggggc cgaactgggc ctcgaacccg gattcgccga ggcgctggcc cggcagacgg
     3301 ttttcggcgc cggccggatg ctccacgagg acaaggaaac cccggcgcgg ctgcgcgaac
     3361 gggtcacctc gcccggcggc accaccgagg cggccctgcg cgtgatggcc gaatccggct
     3421 tcgaaggtat tttgaaacgg gctctggcgc aggcccggcg gcggcccggt gg
//
LOCUS       DAOTFD010000259         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20683_length_3458_cov_4.039083, whole genome shotgun sequence.
ACCESSION   DAOTFD010000259 DAOTFD010000000
VERSION     DAOTFD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20683_length_3458_cov_4.039083"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="P5079_10870"
     CDS             <1..767
                     /locus_tag="P5079_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30521.1"
                     /translation="AYNNMGLIFWKEGRPDLAAKYYSQAIQRKPRNADAWNNLGVVFD
                     EQGKLDMAKDCYVRAVRIAPRFAEAWSNLGIVFGQKREYEQAVRCFRKSLQYRPDRAE
                     FFGNLGMAQAALRRYPEAVRSYESALKRNPGLSGVRTRLGLLLEKQERWEEAETHYRA
                     LLRGGDDPYVSLRLGVVLMRRGEFEESLRHFDRAFLLNPALADARAHLNVGTVLMELA
                     RDEEAEEHLREALRMRPRLKEARDRLRAIRSQRKKE"
     gene            764..3193
                     /locus_tag="P5079_10875"
     CDS             764..3193
                     /locus_tag="P5079_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30522.1"
                     /translation="MSFLEKHFFLTEKGRDRVASAAVALLTFFVFSPALMNGFVNWDD
                     DINFLNNPHYRGLGLSQLKWMWTTYTIGHYSPLTWMSRGLDYLVWGMRPVGYHLTNLL
                     LHAANAVLFYRLARRLFRVAGSAFSERSVRLTAAVSALFFAVHPLRVETVVWATERGG
                     LLSGTFYLACLVFYLRWIESGGGNLRNRNAVWAEVFFALALLSKTSVMALPAVLLLMD
                     VYPLRRLGGERGWFFHPSARRVWMEKIPFLLLALLSLPIAFLGPLRAGAMKGLGQYGL
                     GSRLALVCDGLIFYLQKTFWPVGLSPLYELPSTVRLWSAPHLTHVFLVVLITALVLAI
                     YKRWPFYAVAWGAYGVVLLPVSRVFLAGPQWAADRYTYVSCLPWALLAGAGLVLFLPK
                     KISPALGRGLIVCVFGVLSFLTWRQTRVWRDSETLWRHALSINPRTSLAHINLGIYLH
                     NRGRAEEAAVHYQAALDIDPHSELARANIGNIRMAEGKYSEAMEHYRAALALSPANPG
                     LLNNVGVLLFKAGRSEEAMVWYQKALQIAPNVASTYVNLGNWHFQKRDFPKAIENYET
                     ALRLETRLRAGTPEPPLDPSAREMNPLGAIRGPLSLAQIHMRLAEALYQGERYAEAAE
                     NYRRALVYFPNDSPAMTGLADSCLALGRWEEAASLYEALLRNSDSAEFRYNYGLVLLN
                     MGKPEEALRNWQQALVLEPADADAHVQIGLVLAARNDLSGAERHYEKALEIKPDSPEA
                     HYNLALILLRQGRETVAQKHLQRVVGLRPRFGPVWKLLGELYLKQGDASRAQVCFRKA
                     KSLGVADEESR"
     gene            3196..>3458
                     /locus_tag="P5079_10880"
     CDS             3196..>3458
                     /locus_tag="P5079_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30523.1"
                     /translation="MKPPFPFRTTWVWPVGVAFLTLLVFLPALRNGFVNWDDAANFLN
                     NPHYRGFGWEQLKWMWSTLHMGHYIPLTWMTLGLDYRLWGMNPA"
BASE COUNT          601 a          976 c         1103 g          778 t
ORIGIN      
        1 ccgcctacaa caacatgggg cttatttttt ggaaagaagg ccggcccgac ctggccgcga
       61 aatactattc gcaggccatt cagaggaaac cgaggaacgc cgacgcgtgg aacaatttgg
      121 gcgtggtctt cgacgagcag gggaaactcg acatggcgaa ggattgctac gtccgtgccg
      181 tgcggatagc cccccgtttc gccgaggcgt ggagcaatct cggaatcgtc ttcggacaaa
      241 aacgggaata cgaacaggcc gtccggtgct ttcgaaaaag tctccaatat cgtccggacc
      301 gggcggagtt tttcggcaat ttggggatgg cccaggcggc gttgcgccgg tatcccgaag
      361 ccgtccggag ctacgaatcg gctctgaagc ggaatcccgg gttgtccggc gtgcggaccc
      421 ggctgggctt gctcctggaa aaacaggaac gctgggaaga ggccgagacg cactaccggg
      481 ccctgttgcg cggcggcgat gacccctacg tttccctgcg gttgggagtc gtcctgatgc
      541 ggcgggggga attcgaagag tcgttgcgtc atttcgaccg ggccttcctc ctgaatcccg
      601 ctctggcgga tgcccgcgcc cacctgaacg tgggaacggt cttgatggag ctggcgcggg
      661 acgaggaggc cgaggagcat cttcgggaag cgttgcggat gcgtccccgt ctgaaagagg
      721 cgcgcgatcg gctccgggcg atccggagtc aaaggaaaaa ggaatgagct tcctggaaaa
      781 gcatttcttc ctgacggaga aggggcgcga tcgggtggcc tcggctgcgg tcgccctttt
      841 gacctttttt gtattttcgc cggccctgat gaacggtttc gtgaactggg acgacgacat
      901 caatttcctc aacaaccccc attaccgcgg gctcggcctg agccagttga aatggatgtg
      961 gaccacttac accatcggcc attacagccc gctgacttgg atgtcgcggg ggttggatta
     1021 tttggtgtgg ggaatgcgcc ccgtgggata ccatctcacc aatctgttgt tgcacgcggc
     1081 gaacgcggtg cttttttatc ggctggcccg ccgcttgttt cgggtcgccg ggtccgcttt
     1141 ttcagagcgg tcggtgcgtc tcacggccgc ggtttccgca cttttttttg ccgttcatcc
     1201 gttgcgggtg gaaacggtgg tgtgggcgac ggaacgcggc ggcctccttt ccggaacgtt
     1261 ctatttggcc tgtctcgttt tttatctgcg ctggatcgag agcggcggag gaaatcttcg
     1321 gaacagaaat gccgtgtggg ccgaggtctt cttcgccctg gccttgctgt cgaagacgtc
     1381 cgtcatggcc ttgcccgcgg ttctgttgct gatggatgtt tatcccttgc ggcggctggg
     1441 gggggaacgg ggatggtttt tccacccttc cgcccgccgg gtctggatgg aaaaaatccc
     1501 tttcctcctc ttggccttgt tgagcctccc catcgctttc ctgggtccgc tccgggccgg
     1561 cgccatgaaa ggcttggggc aatatgggct gggatcgcgc ctcgcgttgg tctgtgacgg
     1621 attgattttc tatctccaaa agactttctg gccggtcgga ttgtccccgc tgtacgagct
     1681 gccgtcgacg gttcggcttt ggtccgcgcc gcacctgacg catgtgttcc tcgtcgttct
     1741 gatcacggcg ctggtcctcg cgatctacaa acgttggcct ttttatgcgg tggcctgggg
     1801 ggcctatggg gtcgtgctcc tgccggtctc gagggtcttc ttggcgggtc cccagtgggc
     1861 cgccgaccgc tatacttatg tctcctgctt gccttgggcg ttgctggcgg gagcgggctt
     1921 ggtgcttttc ctcccgaaaa aaatctctcc cgcgttgggc cgcggattaa ttgtgtgcgt
     1981 cttcggcgtt ctctcgtttt tgacgtggcg acagacgcgc gtctggagag actcggagac
     2041 gttgtggcgg cacgccctct ccatcaaccc ccggacatcg ctggcgcaca tcaatctggg
     2101 catctatctg cacaaccggg ggcgggccga ggaggccgcg gttcattatc aggccgccct
     2161 cgacatcgat cctcattccg aactggcccg tgctaacatc ggcaacattc gcatggcgga
     2221 aggaaaatat tcggaggcca tggaacatta tcgggccgcc ctggcccttt ctcccgccaa
     2281 ccccggtctt ctgaacaacg tgggggttct gctgttcaag gcggggcggt cggaggaggc
     2341 gatggtttgg tatcaaaagg ccctccaaat cgctccgaac gtcgcatcga cgtacgtgaa
     2401 tctgggaaat tggcatttcc aaaaacggga tttcccgaaa gccattgaga attatgagac
     2461 ggcgctgcgg ctggagaccc ggttgcgcgc gggaacgccg gagcctccgc tcgacccatc
     2521 ggcgcgagag atgaatccgc tgggcgccat tcggggcccg ctttccctgg cacagattca
     2581 catgcgcttg gcggaagccc tttaccaggg agagagatat gcggaggcgg cggagaatta
     2641 tcgacgtgcc ttggtctatt tcccgaacga ttctcccgcc atgaccggac tcgccgatag
     2701 ctgtctcgcg ttggggcgat gggaagaggc ggcatccctg tacgaggccc ttttgaggaa
     2761 ttcggactcc gccgagttcc gttacaacta cgggctggtt cttttgaaca tgggaaagcc
     2821 ggaggaggcg ttgcggaatt ggcaacaggc tttggtgttg gagcccgctg acgccgacgc
     2881 ccacgtgcag atcgggttgg tgttggcggc gcggaacgac cttagcggtg ccgagcgaca
     2941 ttacgaaaaa gcgttggaaa ttaaaccgga ctctccggag gcacactaca acctggcgct
     3001 gatcctcctg agacagggcc gcgagacggt cgctcaaaaa cacctgcaac gggtcgtcgg
     3061 gttgcggccc cggttcggcc cggtgtggaa acttctcggg gaactgtatc taaaacaggg
     3121 cgatgcgtcc cgagcacagg tctgtttccg taaggcaaaa agcctcggcg tcgccgacga
     3181 ggagtcccgt tgaatttgaa gcctccattt cccttccgga cgacgtgggt ctggccggtg
     3241 ggggtcgctt ttctcacgct tctcgtcttc ctgcccgctc ttcgcaacgg gttcgtcaac
     3301 tgggacgatg ccgcgaattt cctgaacaac ccccattatc gcggcttcgg atgggaacaa
     3361 ttgaagtgga tgtggtccac cctgcatatg ggccactata ttccgctgac ctggatgacc
     3421 ttgggtttgg attatcggct gtggggaatg aacccggc
//
LOCUS       DAOTFD010000260         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_20937_length_3431_cov_3.584123, whole genome shotgun sequence.
ACCESSION   DAOTFD010000260 DAOTFD010000000
VERSION     DAOTFD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20937_length_3431_cov_3.584123"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="thrB"
                     /locus_tag="P5079_10885"
     CDS             complement(<1..945)
                     /gene="thrB"
                     /locus_tag="P5079_10885"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRY30524.1"
                     /translation="MKIFPPPSPVFTVKVRVPATSANMGAGFDAVGIALKHYNEVELS
                     AWNSDLMPPVTVQIDGEGAAVLPLDGTNLVCRMVRQVMDRCGRKFKAIRVKLTNRIPL
                     ARGLGSSSAAIVGGLVAANVGCGTPFSESEILEMASAQEGHSDNVAPVLLGGVCVCAG
                     VEGKTRYASWKSARLFTGLRAVLCVPYFQLPTQEARGVLPDRVSREDAVFNSSRVGLF
                     FSAFHLRRFELLGDAMDDRIHQPYRKKLVPGLGNVLKAARFAGAWGVALSGSGPSVVA
                     LSPVKSARSVAAAMIEAFREKGVAAIPMDLEIDVRGAQV"
     gene            complement(942..2477)
                     /gene="thiC"
                     /locus_tag="P5079_10890"
     CDS             complement(942..2477)
                     /gene="thiC"
                     /locus_tag="P5079_10890"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRY30525.1"
                     /translation="MWNCRRVSSRTRSSNADGNGTGSSSANEREEDEMSRSPSKRGGN
                     VTQRYRARRGEITPEMRFVAKREKADPEFVRSEVARGRAIIPANVNHLRHRLEPIIIG
                     INFNCKINANIGNSATTSCLKDELRKVDMAVKYHADTVMDLSTGANLDDTRAAIVKHS
                     RVPVGTVPIYGALSRVGKAEDLSPSLVLEEIEKQARQGVDYMTIHAGVLREHVSLTKK
                     RLTGIVSRGGALLAQWMSAHGRQNFLYTHFEDICAIFKKYDVAFSLGDGLRPGSLHDA
                     NDEAQFAELKVLGDLTLKAWEHDVQVMIEGPGHVPLHLVQMNADLEQKWCHEAPFYTL
                     GPIVTDVAPGYDHITSAIGGALIGWAGASMLCYVTPAEHLSLPDAEDVRQGCIAHKIA
                     AHAADLARGRRGARDWDDAISKARYEFRWKDQFALAMDPSTAKKKHDATLPAESYKDA
                     RYCSMCGPEFCSMRLSRDAHKILKERETPLSLGTGKVSKAPRCKAAKNAVSPRKREPV
                     SAR"
     gene            complement(2395..3075)
                     /gene="thiE"
                     /locus_tag="P5079_10895"
     CDS             complement(2395..3075)
                     /gene="thiE"
                     /locus_tag="P5079_10895"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRY30526.1"
                     /translation="MVHPPKRLSTFTDVSLYCITTAPDKGQSYAAMVEAACAGGADVI
                     QLREKSMGSRELFNLCRELKVLCDKAGSLFILNDRVDVAVAADLDGVHVGQDDLPVEQ
                     VRRILGYRKLIGCSTHSIEQAFQAQTEGADYVSCGPVFATPTKPDYAPVGLGLVKQYR
                     TMLRIPFVAIGGIDTTNLDTVVRAGADRVAVVRAVCGAKDVELSARQFKDKIVQCRRE
                     RNREFVRK"
     gene            complement(3085..>3431)
                     /locus_tag="P5079_10900"
     CDS             complement(3085..>3431)
                     /locus_tag="P5079_10900"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRY30527.1"
                     /translation="GLRVLEDTARFVWETSSLFRKLREIRHALDRTTRSAYPTLLAAR
                     ASVRDMGRTMKETPARSWRGLVVSNFRRTQEALRVLEEYGKTISPAAAPRFKAMRFRL
                     YSAEKQTIKKFF"
BASE COUNT          608 a         1181 c         1018 g          624 t
ORIGIN      
        1 cacctgcgcc ccgcgaacgt ctatctccaa atccatggga atggccgcca cccctttttc
       61 ccggaaagcc tcgatcatgg cggcggccac cgatcgggcg gatttcaccg gagacaacgc
      121 gaccacgctc ggtccggagc ccgacaacgc cacgccccag gcgcccgcaa aacgggcggc
      181 cttcaaaacg tttcccagcc ccggcaccag ctttttccgg tagggctgat ggatccggtc
      241 gtccatggcg tcgccgagaa gctcgaaccg ccgaagatga aaggcgctga aaaaaaggcc
      301 cacccgggaa ctgttgaaca ccgcgtcctc ccgcgagacc cggtccggca ggaccccgcg
      361 cgcctcctgc gtggggagct ggaaataggg gacgcacagc accgcccgca gcccggtgaa
      421 gagacgggcc gatttccagg aggcgtatct cgtcttcccc tccacgccgg cacagacaca
      481 gacgccgccc aagagcaccg gcgccacgtt gtcggaatgg ccctcctgcg ccgaggccat
      541 ctccaaaatc tcactctcgg aaaaaggagt cccgcagccc acgttggccg cgaccaatcc
      601 gccgacgatg gccgcggagg aggaacccag tccccgcgcc aacgggattc gattggtcag
      661 cttgacccga atcgccttga atttccgacc gcatcggtcc atgacctgcc gcaccatgcg
      721 gcacaccagg ttcgtgccgt ccaacggcaa caccgcggcg ccttcaccgt cgatctggac
      781 cgtcacgggc ggcatcagat cgctgttcca agcgctcagt tccacctcgt tgtaatgctt
      841 gagcgcgatt cccaccgcat cgaacccggc gcccatgttg gcggaggtgg ccgggacccg
      901 taccttcacg gtgaacaccg gagacggggg cgggaaaatc ttcatcgggc cgacaccggt
      961 tcccgtttcc gcggcgacac ggcgttcttc gccgccttgc agcgcggcgc cttcgaaacc
     1021 ttccctgttc caagcgacag cggcgtttcc cgttccttca aaatcttatg ggcgtcccgg
     1081 ctgagccgca tggaacagaa ctccggcccg cacatggagc agtatcgcgc gtccttataa
     1141 gactcggcgg gcagggtggc gtcgtgtttc ttcttcgccg tcgacggatc catggccaac
     1201 gcgaactgat ccttccagcg gaactcgtag cgcgccttgg aaatggcgtc gtcccagtcc
     1261 ctggcgccgc ggcggccgcg cgccaggtcg gcggcatggg cggcgatctt atgggcgatg
     1321 catccctgac gcacgtcctc ggcgtcgggc aacgaaaggt gctccgccgg ggtcacgtag
     1381 cacagcatgg aggcccccgc ccagccgatc aacgcgccgc cgatggccga ggtgatgtgg
     1441 tcgtagccgg gggccacgtc ggtgacgatc ggccccaggg tgtagaacgg cgcctcatgg
     1501 caccatttct gttccaaatc cgcgttcatc tgcaccaggt gcagcggcac gtggccggga
     1561 ccttcgatca tcacctgcac gtcgtgttcc caggccttca gggtcaggtc gcccagcacc
     1621 ttcagttcgg cgaactgggc ctcgtcgttg gcgtcgtgca gggagccggg ccgcaggccg
     1681 tcgccgaggg aaaacgcgac gtcgtatttt ttgaagatcg cgcagatatc ctcgaaatgc
     1741 gtgtataaga agttctgccg gccgtgggcc gacatccact gagccaacag cgccccgccg
     1801 cgcgacacaa tgccggtgag ccgttttttc gtcagcgaaa cgtgctcgcg cagaacgccg
     1861 gcgtggatgg tcatgtaatc caccccctgc cgcgcctgtt tctcgatctc ttccaacacc
     1921 aaggagggcg aaaggtcctc cgccttgccc acgcgggaca gcgcgccgta aatcggcacc
     1981 gtgcccacgg gcacccgact gtgcttgacg atggccgcgc gcgtgtcgtc caggtttgcg
     2041 ccggtggaga ggtccatcac cgtgtcggcg tgatatttga cggccatgtc caccttgcgc
     2101 agttcgtcct tcaggcacga ggtggtggcg gagttgccga tgttggcgtt gatcttgcag
     2161 ttgaaattga tgccgatgat gatcggctcc agccggtgcc gtaaatggtt gacgttggcc
     2221 ggaatgatgg cgcggccgcg cgccacttcc gaacggacga attccggatc ggctttctcc
     2281 cgtttggcca cgaagcgcat ctccggggta atctcccccc gacgcgcccg gtacctctgc
     2341 gtcacatttc ccccccgttt ggagggtgaa cggctcattt catcttcctc ccgctcattt
     2401 gcggacgaac tcccggttcc gttcccgtcg gcattggacg atcttgtcct tgaactgacg
     2461 cgccgacagt tccacatcct tcgcgccgca caccgcccgg accacggcca cgcggtcggc
     2521 cccggcccgg accacggtgt ccaggttggt cgtgtcgatc ccgccgatgg ccacgaacgg
     2581 gatgcgcagc atggtacgat actgtttcac cagtcccagc cccaccggcg cgtaatccgg
     2641 cttggtgggc gtggcgaaca ccggcccgca actcacgtaa tccgcccctt cggtctgggc
     2701 ctggaacgcc tgctcgatcg agtgggtgga acagccgatc aatttgcggt atcccaatat
     2761 ccggcgcacc tgttccaccg gcaaatcgtc ctggcccaca tggacgccgt ccaggtccgc
     2821 ggcgacggcc acgtccacgc ggtcgttcag gatgaaaagc gaccccgcct tatcgcagag
     2881 gaccttgagt tcccggcaga gattgaaaag ttcccgggac cccatgctct tttcgcgcag
     2941 ctgaatcacg tccgcgccgc ccgcgcacgc cgcctccacc atcgccgcgt aggattgccc
     3001 cttgtcgggc gcggtggtga tgcagtagag cgaaacgtcc gtgaacgtag acagccgttt
     3061 gggtggatga accatccctc tccctcaaaa aaactttttt atcgtctgtt tctccgccga
     3121 ataaaggcga aagcgcatcg ctttgaaccg cggcgccgcc gccggcgaaa tcgtttttcc
     3181 gtattcttcc agcacccgca gggcctcctg cgtccgccga aaattggaga ccaccaaccc
     3241 ccgccaggaa cgcgccgggg tttccttcat cgtccgcccc atgtcccgga cgctcgcgcg
     3301 cgccgcgagc agcgtcggat aggctgaacg cgtggttctg tccagcgcgt gtcgaatctc
     3361 ccgcagtttt cggaaaagag acgacgtctc ccagacgaaa cgggcggtgt cttccagcac
     3421 ccgcaggccc t
//
LOCUS       DAOTFD010000261         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21011_length_3423_cov_4.715261, whole genome shotgun sequence.
ACCESSION   DAOTFD010000261 DAOTFD010000000
VERSION     DAOTFD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21011_length_3423_cov_4.715261"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..797)
                     /locus_tag="P5079_10905"
                     /pseudo
     CDS             complement(162..797)
                     /locus_tag="P5079_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415680.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(821..1369)
                     /gene="atpH"
                     /locus_tag="P5079_10910"
     CDS             complement(821..1369)
                     /gene="atpH"
                     /locus_tag="P5079_10910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRY30528.1"
                     /translation="MAKDLTAARCYARAYFQAASAEKNEEKACRDMGQFVRLVDKDEK
                     LARTLDHPFLPAAEKKKAVLSKMKSASPVFGRFLELLIVKKRVSFAARVAELLEEMLD
                     EARGVQRMRVKSAAPLAAEQAAALEKEFGKLWNKQAKAELFVDESLVAGLLVQAGDRV
                     WDASLKGRLERLRERMLQSARN"
     gene            complement(1386..1895)
                     /gene="atpF"
                     /locus_tag="P5079_10915"
     CDS             complement(1386..1895)
                     /gene="atpF"
                     /locus_tag="P5079_10915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRY30529.1"
                     /translation="MEKLLNPDTGLMIWTVLTFAVLVFVLGRFAWKPLLESLAAREDR
                     IRADVKAAEEARLAAEKMRNDYQDQLAAVEGKTRELIARAQAEADRLREDMVKTAQGE
                     SVKLMEKTRRQMEEEQRRLVRELRTEVADLSVAAAEKLLRQAVDKSVKEKFVRQALSD
                     FDKMSKDIH"
     gene            complement(1937..2224)
                     /gene="atpE"
                     /locus_tag="P5079_10920"
     CDS             complement(1937..2224)
                     /gene="atpE"
                     /locus_tag="P5079_10920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRY30530.1"
                     /translation="MLYLGLGYLGAGLGAGLCLLAAAFGIARLASAALEGTARQPEAA
                     GVLRTSMIIPAALIEGLGFFALVICLLVALNLGLPKGEAASGAAAATETAH"
     gene            complement(2286..3038)
                     /gene="atpB"
                     /locus_tag="P5079_10925"
     CDS             complement(2286..3038)
                     /gene="atpB"
                     /locus_tag="P5079_10925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRY30531.1"
                     /translation="MNFTEILEHHLLDHAYFRLPAVAGVSLPVSKHVVMLWIAGALAV
                     LFFVPVGLAARAGRRNTVVGMVEAVAVFVRDEIVLPNLGEDGRRYLPYFLSLFFFILF
                     CNLLGMVPFGATATGNIAVTAALALTTFAMINLAGMRAQGVGPYVKNLVPHGLPVWLL
                     PLMFPIEILGLVTKSFALCIRLFANMIAGHIVILAFMALIFLFGSVLVAPVSVAAAVG
                     MDLLEIFVAFLQAYIFTLLSAIFIGAAVHPQH"
     gene            complement(3035..>3423)
                     /locus_tag="P5079_10930"
     CDS             complement(3035..>3423)
                     /locus_tag="P5079_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30532.1"
                     /translation="IFPTEMWKNRALGGLALTVGACLGLGAAAIFFQGFRTSLVLGGS
                     VALVLVLPSYFSLAWALHKTDKMFYSVFAAGILYRLTGLAFTAWMVWRLARPRTAAVL
                     LSAVGLVILLTPLELYFFQKEGRKSP"
BASE COUNT          657 a         1235 c          927 g          604 t
ORIGIN      
        1 taataattat ttggtatatt taataattac tatgcctgat cctccacttc ctccaggtct
       61 agatgtacca tgtccaccac cacctcctgc tccagaattt gcagctgcag aacttccatt
      121 accattatta cccccatttc caccattgcc accaaaccca cctgcaccac ctgcgccacc
      181 gtggactgtt tttgtccgac ggccacgtag atgcagaggg ggcggttcgg ttcgtccttc
      241 tgcttgatga tggcgtccac ggcgatggcg gtcttccccg tctgccggtc gccgatgatc
      301 agttcccgct gtccgcgtcc gatggggatc atcccgtcga tggccttgag gccggtctgc
      361 agaggctcct tcaccggctg gcgttccagc acgcccgggg cgatgacctc gatgggtcgc
      421 gtcccggacg atttcacggg cccgcgaccg tccagcggct gcccgagcgg gttgaccacc
      481 cggccgatga gcgcctcccc cacggggact tccatcacgc ggccggtgcg ctgcaccggg
      541 tcgccctcct tgatcagcgt gtcctcgccg aagagcacgc accccacgct ctcccgttcc
      601 aggttcagca ccatgccgaa caccccgtgc gggaactgca gcatctcgcc ggccacggcg
      661 ttctgcaggc cgtagaggcg ggcgatgccg tctcccacct gaagcacgta gccgacctcc
      721 cggagttccg tctccggctc aaatccttcg atgcgttcct tcagaacgcg tgtgatctct
      781 tccggtttta tcgccatggt cctcgagtcc cctccccgcg tcaattcctt gcggactgaa
      841 gcatccgttc ccgcaaacgt tccagccgtc ccttcagact ggcgtcccac acccggtcgc
      901 cggcctgcac cagaagaccc gccaccaggc tctcatccac gaaaagttcc gccttcgcct
      961 gcttgttcca aagttttccg aactcttttt ccaacgccgc cgcctgttcc gccgcaaggg
     1021 gcgcggcgct tttgacccgc atccgctgca ccccgcgcgc ctcgtcgagc atctcttcca
     1081 aaagttccgc cacccgggcc gcgaaagaaa ccctcttctt gacgatgagg agttcgagga
     1141 accgcccgaa aacgggggag gcggatttca tcttcgacaa aacggctttt ttcttctcgg
     1201 cggccggcag gaacgggtgg tccagcgtcc gcgccaattt ttcatccttg tcgacgaggc
     1261 ggacgaactg ccccatgtcg cggcaggcct tctcctcgtt cttttccgcg gaggccgcct
     1321 ggaaatacgc ccgcgcgtaa catcgggccg cggtcaggtc cttcgccatc cgtcttcccc
     1381 gcccttcagt ggatgtcttt cgacatcttg tcgaaatccg acagcgcctg gcgcacgaac
     1441 ttctctttga cggatttgtc caccgcctga cgcaggagtt tttccgcggc cgccacggag
     1501 aggtccgcca cctccgtccg caattcccgg acgagccgcc gctgttcctc ctccatctgc
     1561 cgccgggtct tctccatcaa cttcaccgat tccccctgcg ccgtcttcac catgtcctcc
     1621 cgaagccggt ccgcctccgc ctgggcgcgg gcgatcagct cgcgggtctt gccttccacg
     1681 gcggcgagct gatcctgata atcgttcctc atcttctcgg ccgccaggcg cgcctcctcc
     1741 gccgccttca cgtcggcgcg gatacggtcc tcccgggccg ccaacgattc caggagcggc
     1801 ttccaggcga accggccgag gacgaacacc agcaccgcga acgtcagcac ggtccatatc
     1861 atcagcccgg tgtcggggtt caggagtttt tccaaaagac gttccttcct ttaaggatgc
     1921 cggacggtcc cgcggctcag tgcgccgtct ccgtcgcggc cgccgcgccg gaggccgcct
     1981 cgcctttggg aagaccgagg ttcagcgcca ccagcaggca gatcaccagg gcgaaaaaac
     2041 ccagaccttc gatcagggcg gcggggatga tcatggacgt ccgcaaaacg cccgccgctt
     2101 ccggctgccg ggcggtccct tccaacgccg cgctcgccag ccgggcgatg ccgaacgccg
     2161 ccgccaaaag gcacagtccc gcgccgagcc cggcgcccaa atatcccaat cccagataca
     2221 acatttccat gagttcctcc ttctcgtatt cgacccgaag attcaaattc ggggatgctt
     2281 caaaatcaat gctgcggatg gaccgccgcg ccgatgaaga tggccgagag cagggtgaag
     2341 atgtaggcct gcagaaaagc cacgaagatc tccagcaggt ccatgcccac cgccgccgcc
     2401 accgacaccg gcgccaccag cacgctgccg aacaggaaaa tcagcgccat gaaggccagg
     2461 atcacgatgt ggccggcgat catgttggca aacaaccgga tgcacaacgc gaaactcttc
     2521 gtcaccagcc ccaatatctc gatggggaac atgagcggca ggagccatac cggcaagccg
     2581 tgcggcacca agtttttcac gtaggggccg accccctgcg cccgcatccc ggccaggttg
     2641 atcatggcga aggtggtcag ggccagcgcg gccgtcaccg cgatgttccc cgtggcggtc
     2701 gcgccgaacg gcaccatccc cagcaggttg cagaacagga tgaaaaagaa cagggacaga
     2761 aaatagggga gataccgccg gccgtcctcc cccaggttgg ggaggacgat ctcgtcccgc
     2821 acgaacaccg ccaccgcctc caccatcccc acgacggtgt tgcgccgccc cgcccgggcg
     2881 gccaggccca ccggcacgaa aaacaggacg gccaacgccc cggcgatcca cagcatgacc
     2941 acatgtttgg aaacgggcag ggacaccccc gccaccgcgg gaagccggaa atacgcgtgg
     3001 tccaacaggt ggtgttcgag gatttccgtg aagttcacgg cgacttccgc ccctcctttt
     3061 gaaaaaagta gagctccagc ggcgtcaaca ggatcaccag ccccaccgcc gacaacagaa
     3121 ccgcggccgt ccgcggacgg gccaggcgcc agaccatcca cgccgtgaac gccaacccgg
     3181 tcaaacggta caatatcccc gcggcgaaca cggaatagaa cattttgtcc gttttatgca
     3241 gggcccacgc caaactgaaa tagctgggca acaccaggac cagcgccacc gaaccgccca
     3301 ggaccaacga ggtccggaac ccttgaaaaa aaatcgccgc cgccccgagg cccaggcacg
     3361 cgccgaccgt cagcgccaga ccgcccagcg ccctattttt ccacatctcg gtcggaaaaa
     3421 tac
//
LOCUS       DAOTFD010000262         3403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21203_length_3403_cov_3.627240, whole genome shotgun sequence.
ACCESSION   DAOTFD010000262 DAOTFD010000000
VERSION     DAOTFD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21203_length_3403_cov_3.627240"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /gene="leuS"
                     /locus_tag="P5079_10935"
     CDS             complement(<1..1859)
                     /gene="leuS"
                     /locus_tag="P5079_10935"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRY30533.1"
                     /translation="MADTTYDHSQIEPKWQKRWAEAGLFRAQDGESGKPKIYILDMFP
                     YPSAAGLHVGHPEGYTATDIIARMKRMQGHNVLHPMGWDAFGLPAENYAIQNRVHPRD
                     VTKNNIDNFRRQIQALGFSYDWAREVDTTDPAYYKWTQWIFLQLFNSYYDPEKQKLFP
                     IALLQQQGMPPEEIGKRRLAYEGVSPINFCPKCKTGLANEEVHQGGCERCGSPVERRD
                     MRQWILKITAYADRLLEDLNGLDWPESTLAMQRNWIGRSEGAQAAFAAVSPKSVEGET
                     VTVFTTRPDTLFGATYMVLAPEHPLVDKFTAPEQKTAVDEYRRKARAKSDLERTDLAK
                     EKTGVFTGGYVKNPVNAERIPVWVADYVLISYGTGAIMAVPAHDDRDYEFAKKFNLPI
                     RQVVSAPGADISTQAFIGDGAAINSGEFTGLPTAEFKKKIIDWLEKRGLGRKAVHYRL
                     RDWVFSRQRYWGEPIPVVHCRGKCNGVVPVPEDQLPVLLPDVRNFEPTGTGDSPLAAM
                     DRWVETACPACGGPARRETNTMPQWAGSCWYYLRFLDPRNAAAPWDKSKEKYWMSDKG
                     VDLYVGGSEHAVLHLLYARFWHKVLHDLGWVTTKEPFQKLRLQGMVLAYSYRD"
     gene            complement(1868..2626)
                     /locus_tag="P5079_10940"
     CDS             complement(1868..2626)
                     /locus_tag="P5079_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRY30534.1"
                     /translation="MTTPRAKRAEWPRLDHLLVERGLAESGAKAQALILAGRVRAPGL
                     KTVKPGTRVPWDQPVELTETLRYVSRGGEKLEGALRDLKIDVHGRACLDVGASTGGFT
                     DCLLQHGAQKVLAVDVGHGQLHWKLRNDPRVTGREGTHALRLTPEDLTDFSPDLVTVD
                     VSFISLEKILPHLSVLLREGTKCLALVKPQFEAGPKCAPKGVVRDEKVRTEIIERLKG
                     LLPGWGFSLKSETPAALKGPKGNQEHFLYFIKDS"
     gene            complement(2640..>3403)
                     /locus_tag="P5079_10945"
     CDS             complement(2640..>3403)
                     /locus_tag="P5079_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY30535.1"
                     /translation="FAAGLACGGLKPVVAIYSTFLQRSFDQMMHDVALQKLPVVFCLD
                     RAGIVGDDGPTHHGTFDLSFTRVLPHFIVMSPKDENELQHMLKTALNQPGPVALRYPR
                     GNGVGVPLDTEFRLLPLAKGELLREGKDVTLVAIGEPVQSCLETAKILERQGISAAVV
                     NARFAKPLDKAFYLGLARRAPLLVTVEENTVVGGFGGALRELLEGERVEIRTIALPDG
                     FVEHGSQPKLREKYGLTADKIAERVTELLKQRTAP"
BASE COUNT          548 a         1029 c         1130 g          696 t
ORIGIN      
        1 ttgtcccggt agctgtaggc cagaaccatg ccctggagcc ggagtttttg gaacggttcc
       61 ttcgtcgtca cccagccgag gtcgtggagg accttgtgcc agaatcgcgc gtagagcagg
      121 tgcaggacgg cgtgttcgga accgcccacg tagaggtcca cccccttgtc ggacatccaa
      181 tatttttctt tggacttgtc ccacggcgcg gcggcgttcc ggggatcgag gaagcgcaga
      241 taataccagc aggaaccggc ccattggggc atggtgttgg tctctcgccg ggccggcccg
      301 ccgcacgccg ggcaggcggt ctcgacccac cggtccatgg cggccagcgg ggagtcgccc
      361 gtgccggtgg gctcgaagtt ccggacgtcg ggcaggagga cggggagctg gtcttccggg
      421 accggcacca cgccgttgca tttgccccgg caatgcacca cggggatggg ttcgccccag
      481 taacgttgtc gagagaacac ccagtcgcgc agccggtaat ggacggcttt ccggccgagc
      541 ccgcgttttt cgagccagtc gatgatcttt ttcttgaact ccgccgtcgg cagaccggtg
      601 aactcgccgc tgttgatggc ggcgccgtca ccgatgaagg cttgtgtcga gatgtccgcc
      661 cccggggcgg agacgacctg gcggatgggc aggttgaact tttttgcaaa ttcatagtcg
      721 cggtcgtcgt gggccggcac ggccatgatg gcgccggtgc cgtagctgat gagcacgtaa
      781 tcggccaccc agacggggat gcgctcggcg ttgacgggat ttttgacgta cccgcccgtg
      841 aaaacgcccg ttttctcctt ggccaggtcg gtgcgttcca ggtcggattt ggcgcgggct
      901 ttccggcgat attcgtccac cgcggttttt tgttccgggg cggtgaactt gtccaccagc
      961 gggtgttcgg gcgcgagcac catgtaggtg gcgccgaaga gggtgtccgg gcgcgtggtg
     1021 aaaaccgtga cggtttcccc ctccacgctc ttgggcgaaa cggcggcgaa cgccgcttgg
     1081 gcgccttcgg aacggccgat ccagttgcgt tgcatggcca gggtggattc cggccagtcc
     1141 agtccgttca ggtcttccag gaggcggtcg gcgtacgcgg tgatcttcaa tatccattgg
     1201 cgcatgtcgc ggcgttccac ggggctgccg caccgttcgc atccgccctg gtgcacctcc
     1261 tcgttcgcca ggccggtctt gcatttgggg cagaagttga tggggctgac gccctcatag
     1321 gcaagccttc gcttgcctat ctcttccgga ggcatcccct gttgctgaag gagcgcaatg
     1381 gggaagagtt tttgtttttc gggatcgtag tatgaattga aaagttgcaa gaaaatccac
     1441 tgggtccatt tgtaataggc cgggtcggtg gtgtcgacct cgcgggccca gtcgtaggag
     1501 aacccgagcg cctggatctg ccgacggaaa ttgtcgatgt tgtttttggt gacgtcgcgc
     1561 ggatggacgc ggttctgaat ggcgtagttc tcggcgggca gtccgaaggc gtcccagccc
     1621 atgggatgga gcacgttgtg gccctgcatg cgtttcatgc gggcgatgat gtcggtggcg
     1681 gtgtatcctt ccggatgccc cacgtgcagc cccgccgccg acgggtacgg gaacatgtcg
     1741 aggatgtaga tcttgggttt ccccgactcg ccgtcctggg ctcggaacag ccccgcctcg
     1801 gcccagcgtt tctgccattt gggttcgatt tgactgtgat cgtaggtcgt gtctgccatc
     1861 ggagtcctta agagtcttta atgaaatata aaaaatgttc ctggttgcct ttgggccctt
     1921 tcagggccgc cggcgtttcg cttttgaggg agaatcccca tcccggcaga agccccttga
     1981 ggcgttcgat gatttcggtg cggaccttct cgtcccgcac cacgcctttg ggcgcgcatt
     2041 tcggcccggc ctcgaactgc ggtttcacca gcgcgaggca cttcgtgccc tcccgcaaaa
     2101 ggaccgacag gtgcggcaaa attttttcca gagagatgaa cgacacgtcc accgtcacga
     2161 ggtccggcga aaaatcggtc aggtcttccg gcgtcaggcg cagcgcgtgc gtgccttcgc
     2221 ggccggtcac gcgggggtcg ttccgcagtt tccagtggag ctggccgtgt cccacgtcca
     2281 cggccaggac tttttgcgcg ccgtgctgca acaggcaatc cgtgaaaccg cccgtggagg
     2341 cgcccacgtc caaacaggcg cggccatgaa cgtcgatctt caaatcgcgt aaagcgccct
     2401 cgagcttctc cccgccgcgg gaaacgtaac gcagggtttc ggtcaattcc accggctggt
     2461 cccaggggac ccgggttccc ggtttgacgg ttttcaatcc gggcgcccgg acgcggccgg
     2521 ccaggatgag cgcctgcgct ttggcgccgc tctcggccag cccccgctcc accaacagat
     2581 ggtccagccg gggccattcg gcccgtttcg cacggggcgt ggtcacgcgt ttccggcgct
     2641 cagggggccg tgcgttgctt cagaagctcc gtcacccgct cggcgatttt gtcggcggtg
     2701 aggccgtact tttcgcggag tttcggctga gagccgtgtt cgacgaaccc gtccggcagg
     2761 gcgatggtgc gaatctccac acgttcgcct tccagaagtt cccggagggc gccgccgaat
     2821 ccgccgacaa cggtgttttc ctccaccgtg acgaggagcg gagcccgccg ggccagtccg
     2881 agataaaagg ccttgtccaa aggtttggcg aaacgggcgt tgaccacggc ggccgaaatc
     2941 ccctgtcgtt ccaggatctt cgcggtttca agacaggact gcaccggttc gccgatggcc
     3001 acgagggtga cgtctttccc ctcccgcagg agttccccct tggccagggg gagcaggcgg
     3061 aattcggtgt cgagcggcac gcccaccccg ttgccgcgcg gataacgcaa cgccacgggg
     3121 cccggctgat tgagggcggt tttgagcatg tgctggagtt cgttctcgtc cttgggagac
     3181 atcacgatga aatgaggcag gacgcgggtg aagctgaggt caaaggtgcc gtgatgggtg
     3241 gggccgtcgt cgccgacgat gccggcgcgg tccaggcaga acaccaccgg cagtttctgc
     3301 agcgccacgt cgtgcatcat ctggtcgaaa ctgcgctgga ggaaggtgga atagatcgcc
     3361 accaccggtt tcagcccgcc gcaggccagc ccggccgcga acg
//
LOCUS       DAOTFD010000263         3392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21290_length_3392_cov_4.528319, whole genome shotgun sequence.
ACCESSION   DAOTFD010000263 DAOTFD010000000
VERSION     DAOTFD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21290_length_3392_cov_4.528319"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2662)
                     /gene="nifJ"
                     /locus_tag="P5079_10950"
     CDS             complement(<1..2662)
                     /gene="nifJ"
                     /locus_tag="P5079_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRY30536.1"
                     /translation="MSKNYAMMDGNEAAASVAYRASEVIAIYPITPSSNMGEWADAWA
                     AAGAKNIWGTVPLVQEMQSEAGAAGAIHGALQAGALATTFTASQGLLLMIPNMYKIAG
                     ELTPTVFHVAARSLAAQALSIFGDHSDVMATRATGYALLCSSSVQEAMDFALLAHAAS
                     LETRVPFLHFFDGFRTSHEVSKISPVSDEVIRAMIDEKLVREHRARCLNPNDPVLRGT
                     AQNPDVYFQARESVTPYYQKCPALLQKAMDKFASLTGRQYRLFEYHGSPDAERVIVLM
                     GSGAEAVHETVDALAAHGEKVGVLKVRLYRPFDNNLFLAGLPQSAKVVAVLDRTKEPG
                     SAGEPLYLDAVNAMVEGRRAGLSVLGGRYGLSSKEFTSSMVKGVFDEMKKDAPKKHFT
                     VGIKDDVSGSSVDPDDSFSTEADDVVRAVFFGLGADGTVSANKNSIKIIGEVTDKYTQ
                     GYFVYDSKKAGTITVSHLRFGPRPIRSTYLVSRANFVGCHQFGFLYKYDVVEKLVPGG
                     VFLLNSPYGAEEVWDRLPKHVQKLLIDKKIKFYVIDAYRVARETGMGVRINTIMQTCF
                     FAISNILPKDQAISYIKTYIKKEYGKKGDEIVNKNYNAVDQTLAHLKEVTIPSKVTAT
                     TEAPPVVAEEAPEFVKNVLAPIIAGHGDDLPVSAFPVDGTYPLGTSKWEKRNIAQEIP
                     VWDESVCIQCNKCVIVCPHAVIRAKVYDTKLLAGAPETFKSTPSKNREWQGMVYTLQV
                     APEYCTGCAMCVDICPAKNKSETRLKAINMKPQPPLREPESKNWDFFLKLPEFDRRQV
                     KRSEVRGSQLFEPLFEFSGACAGCGETPYIKLMSQLFGDRAVVANATGCSSIYGGNLP
                     TTPWTKNAAGRGPAWSNSLFEDNAEFGLGFR"
     gene            complement(2744..3331)
                     /locus_tag="P5079_10955"
     CDS             complement(2744..3331)
                     /locus_tag="P5079_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY30537.1"
                     /translation="MQKKISVIVAHPNPKSFNHAIAGQAAATLRQLGHAVFYHDLYRE
                     KFDPLFGVGELQSSGPIPRAIGLYCREALESDGFVVVHPNWWAQPPAVLKGWVDRVLR
                     QGLAYRFTDEGKVVGLLKAPTAVVFNTANTPEEAEREMFGDPLDGLWKKCIFEFCGVK
                     NVHREVYRPVIVSSAIQRAQWLASAREVVAACFPA"
BASE COUNT          622 a         1034 c         1043 g          693 t
ORIGIN      
        1 gccggaaacc gagtccgaac tcggcgttgt cctcgaacag ggagttggac caggccgggc
       61 cgcggcccgc cgcgttcttg gtccacggcg tggtgggcag gttgccgccg tagatggagg
      121 aacacccggt ggcgttggcc accacggcgc ggtcgccgaa aagctgggac atcagcttga
      181 tgtagggcgt ttcgccgcaa ccggcgcagg cgccggagaa ctcgaacaac ggctcgaaca
      241 gttgcgatcc gcgcacctcg gaacgtttga cctggcgccg gtcgaattcc ggcaatttca
      301 ggaaaaaatc ccagttcttg ctctccggct cgcgcagcgg cggctgaggt ttcatgttga
      361 tggccttcaa gcgcgtctcc gacttgtttt tcgcgggaca gatgtccacg cacatcgcgc
      421 agccggtgca atattccggc gccacttgca aggtatagac catcccctgc cactcccggt
      481 tcttggacgg tgtggacttg aaggtctccg gcgcgcccgc cagaagtttc gtgtcgtaca
      541 ccttggcccg gatgacggcg tgcggacaga cgatgacgca cttgttgcac tggatgcaaa
      601 cgctctcgtc ccacacgggg atttcctggg cgatattgcg tttctcccat ttcgacgtcc
      661 ccagggggta ggtgccgtcc accgggaacg cgctcacggg aagatcatcg ccgtgtcccg
      721 cgatgatggg ggccaggacg ttcttcacga attccggggc ctcctcggcc acgaccggcg
      781 gggcctccgt cgtcgcggtc accttcgacg ggatcgtcac ctccttgagg tgcgcgagcg
      841 tctggtccac ggcgttgtag ttcttgttca cgatctcgtc gccttttttc ccgtattcct
      901 tcttgatgta ggtttttatg taggagatgg cctggtcttt ggggaggatg ttggagatgg
      961 cgaaaaaaca ggtctgcatg atggtgttga tgcgcacgcc catcccggtc tcacgcgcca
     1021 cccggtaggc gtcgatgacg taaaacttga tttttttatc gatgagaagt ttctgcacgt
     1081 gtttcggcag gcggtcccat acctcctcgg ccccgtaggg actgttcaac aggaacacgc
     1141 cgcccggcac caacttctcc acgacgtcgt acttgtataa aaacccgaac tggtgacatc
     1201 ccacgaagtt ggcccgactg acgagatagg tggaccggat gggccgcgga ccgaaccgca
     1261 ggtgcgacac ggtgatggtg ccggcctttt tggagtcgta gacgaaatat ccctgggtgt
     1321 atttatcggt gacctcgccg atgatcttga tggagttctt gttggcgctg acggtgccgt
     1381 cggccccgag cccgaagaag accgccctca ccacgtcgtc ggcctcggtg gaaaaagagt
     1441 cgtccgggtc cacgctgctg ccggaaacgt cgtccttgat tcccacggtg aaatgttttt
     1501 tgggggcgtc ctttttcatt tcgtcgaaga cgcccttcac catggaagac gtgaactcct
     1561 tggaggagag gccgtaacga ccgccgagga ccgacaagcc ggcccgacgg ccttccacca
     1621 tggcgttgac cgcgtcgaga tagagcggtt cgccggcgct gcccggctcc ttggtgcggt
     1681 cgagcacggc aacgaccttg gcgctttggg gcaaccccgc cagaaagagg ttgttgtcga
     1741 aaggccggta gaggcgcacc ttgagcacgc ccactttttc gccgtgggcg gcgagagcgt
     1801 ccacggtctc atgcacggcc tcggcgccgg agcccatcaa cacgatgacc ctctcggcgt
     1861 cgggagaccc gtgatattcg aacaaccgat attgccggcc ggtgagggag gcgaacttgt
     1921 ccatggcctt ctgcaacagg gctggacatt tctgataata gggcgtcacg gattcccgcg
     1981 cctgaaaata aacgtccggg ttctgcgccg ttccgcgcag gaccggatcg ttcgggttga
     2041 gacagcgggc gcgatgttcc cgcaccagct tttcgtcgat catggcgcgg atcacctcgt
     2101 cggagacggg ggatattttg gaaacctcgt gcgaggtgcg gaaaccgtcg aagaaatgca
     2161 aaaacgggac gcgggtctcc agggaggcgg cgtgcgccaa cagcgcgaaa tccatggcct
     2221 cctggaccga actggaacag aggagcgcat agcccgtggc gcgggtggcc atcacgtcgg
     2281 aatggtctcc gaagatggac agggcctgcg ccgccaggga ccgggccgcc acatggaaca
     2341 ccgtcggggt aagttcgccg gcgatcttgt acatgttggg gatcatcaaa aggagcccct
     2401 gggaagcggt gaaggtggtg gccaacgcgc ccgcctggag cgcgccgtga atggcgccgg
     2461 ccgcgccggc ttcgctctgc atctcctgca ccaacggcac ggtgccccag atattcttcg
     2521 ccccggccgc ggcccaggca tcggcccatt cccccatgtt ggaggacggc gtaatcgggt
     2581 aaatcgcaat cacctcgctg gcgcggtacg ccacggaagc ggcggcctcg ttcccatcca
     2641 tcatcgcgta attcttgctc attatttttc ctctccgggc tgtgcccttt ccaaatggtt
     2701 ttctatctta cagctctttc tgttttcatt ttctttcgga ccgtcatgcc gggaaacagg
     2761 cggcgacgac ctcccgtgcc gaagccagcc attgggcgcg ctgaatcgcc gaactgacga
     2821 tgaccggtcg atacacctcg cggtgcacgt ttttgacgcc gcaaaattcg aagatgcatt
     2881 ttttccaaag gccgtcgagc gggtcgccga acatctcgcg ttccgcctcc tcgggcgtgt
     2941 tggccgtgtt gaagacgacg gccgtcggcg ccttcaacag accgaccact ttcccctcgt
     3001 cggtgaaccg gtaggccaat ccttgacgca ggacccggtc cacccagccc ttcaacaccg
     3061 cgggcggctg cgcccaccag ttcggatgga cgacgacgaa cccgtcgctc tccagggcct
     3121 cccggcaata aagccctatc gcccggggaa tgggccccga actctgtaat tcgccgacgc
     3181 cgaagagggg atcgaacttt tcccgataaa gatcgtggta aaaaaccgcg tggccgagtt
     3241 gacgaagcgt cgccgcggcc tgaccggcga tggcgtggtt gaaacttttc ggatttggat
     3301 gcgcgacgat gaccgagatt ttcttttgca tgacctcgcc ggaaaaacgt cttccggtcc
     3361 ctcaaccccg tttcagtccc atggagcgcg ag
//
LOCUS       DAOTFD010000264         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21313_length_3389_cov_4.462807, whole genome shotgun sequence.
ACCESSION   DAOTFD010000264 DAOTFD010000000
VERSION     DAOTFD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21313_length_3389_cov_4.462807"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="P5079_10960"
     CDS             complement(<1..759)
                     /locus_tag="P5079_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30538.1"
                     /translation="MSRLLVVEDEQDLRRAIVKELTALGHEVEEAPDGEKAKVMLRTS
                     GYDLVISDLMLKEVNGLDVLREAKVCDPHTETILMTAFGSMESNVEAMKLGASAYLLK
                     PLDMDELGRKVGACLERRNFYLKNQSLETEINVLRASRETATKLMLGSMMRLLLKSAR
                     DILKARGGLLLRTRQGELCVEAATNDFKDDLMGSSLPQLNDLFDMVRANRRPYALEGR
                     HAGDRLAGAHRTPEEISAVILSPILEGDLSFGVFL"
     gene            complement(756..2192)
                     /locus_tag="P5079_10965"
     CDS             complement(756..2192)
                     /locus_tag="P5079_10965"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY30539.1"
                     /translation="MKLWTKFSLFSSGLVALVIVGTTFSMLYLEKRHLLSEMTKGMHE
                     TAKSLQKVSAESFLTNNRVSLISYMKEVRNARGVAFAQVVEKSGKIHAHTDSDRIGRT
                     VDDPFVRQALDAGTPLSREDSAAPGGTVIELAYPVNTGGPEQAAAVIGFSRDALETSV
                     RRTVQETKKRLLHVALIALVFGVLGAVLLAAVMTGPIRRMVAAAEKIGQGKLDTRVEV
                     SSTDELGHLAQSLNAMAVKLHELDQLKQDFVSSVTHEFRSPLSAMKAHFDLFFRGRLG
                     TLTEEQKESLTVLSNNAARLGRFIDDLLDIAKIERGKMDIKPEVFDPGPVLKDIHRLF
                     QPRAEQKGIDLRLELPEKMPPLFADPGRTGQIVTNLVSNALKFTEKGNIALKVEADEK
                     CLHILVSDTGMGIPEDQLDRIFNKFEQVKDARNRTTEQKGTGLGLAIARALAERQGGA
                     LRAQSVLGQGSTFILTVPVHAAHPGAPA"
     gene            complement(2189..>3389)
                     /locus_tag="P5079_10970"
     CDS             complement(2189..>3389)
                     /locus_tag="P5079_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30540.1"
                     /translation="DEDRYARLGLEWVVQNLIALRVGYRERDDLDSGLRYGLGVQTSG
                     LSFDYAFVPRGVFQDGHCFTLSLRYGRHRGYTQAENDIDRQFGRANNLYHRGEYLRAY
                     RILNELLSARPGHGPARDLLAQITLTLEQAETAKVVEGQLKKGRRYYRRQMFIDAATA
                     FKAVLLLDPANAEATRALAEIERRYDEVAESIYKSGVALYAEGRYDEARQHMERVLAF
                     RPGRADAQEQLRLIDEKLKRLEDIRRQQAVELLLRNGQAQFDKGHWEKALDACRQALA
                     LDPSSETARTRAAEIGAKLADKHFAEGVTCYESGDAPAALALFIKALSFDERHDGALT
                     YRRRLEQEKKAADRKKALELNERALVEYGNGQLKKAVGLWENALALDPDLTEARTNLD
                     RARKEMK"
BASE COUNT          525 a         1076 c         1087 g          701 t
ORIGIN      
        1 aaggaacacc ccgaaggaaa gatcgccctc cagaatgggc gagaggatca cggccgagat
       61 ctcttccggc gtccggtggg cgccggcgag ccggtcgccg gcgtgccggc cctccagcgc
      121 ataggggcgc ctgttcgcgc gcaccatgtc gaagaggtcg ttcaactggg ggagggaact
      181 gcccatgagg tcgtccttaa aatcgttggt ggcggcttcc acgcacagtt cgccctgccg
      241 cgtgcggagc aacaatcctc cgcgggcctt caggatgtca cgggccgatt tgagaagcag
      301 gcgcatcatg gagcccagca tgagtttcgt ggccgtttcg cgggaggcgc ggagaacgtt
      361 gatctccgtt tccaggctct ggtttttcag ataaaaattc cgtcgttcca gacaggcccc
      421 gactttcctc cccagctcgt ccatgtccag cggtttcaac agataggcgg aggcccccag
      481 tttcatggcc tccacgttgg attccatgct cccgaacgcc gtcatcagga tggtttcggt
      541 gtggggatcg cagaccttgg cttcccgcag gacgtccagg ccgttcacct ctttcagcat
      601 gaggtcggag atgaccaggt cgtatccgct ggtgcggagc atgaccttgg ctttttcgcc
      661 gtcgggcgcc tcctccacct cgtggcccag cgcggtcagt tccttgacga tggcgcggcg
      721 caggtcctgt tcgtcttcca ccaccagcag gcggctcatg cgggggctcc gggatgtgcg
      781 gcgtgtaccg gcaccgtgag gatgaacgtg ctcccctgtc ccagaacgct ttgcgcccgg
      841 agggccccgc cttgccgttc cgccagcgcc cgggcgatgg ccagtcccag gcccgtcccc
      901 ttctgttccg tggtccggtt ccgggcgtcc ttgacctgct cgaacttgtt gaagatgcgg
      961 tccagctggt cttcggggat ccccatgccg gtgtcgctca cgaggatatg caggcatttc
     1021 tcatcggcct cgactttcag ggcgatgttc cccttctccg tgaacttgag cgcgttggac
     1081 accaggttgg tgacgatctg gcctgtgcgg cccgggtcgg cgaagagggg gggcattttt
     1141 tcgggcagtt ccaagcgcag gtcgatgcct ttctgttcgg cccgcggctg gaagagccgg
     1201 tggatgtcct tgagcaccgg gcccggatcg aagacctccg gcttgatgtc catctttccg
     1261 cgttcgatct tggcgatgtc cagaagatcg tcgatgaacc ggcccaggcg cgcggcgttg
     1321 ttggacagca ccgtgaggga ttccttctgt tcctcggtga gcgttcccag ccgcccgcgg
     1381 aagaaaaggt cgaaatgcgc cttcatggcg ctcagggggg agcggaactc gtgcgtcacg
     1441 ctggacacga aatcttgttt cagctggtcc agttcgtgga gtttcacggc catggcgttg
     1501 agcgactgcg ccaggtgccc cagttcgtcg gtcgaggaga cttccacgcg ggtgtccagt
     1561 ttgccctggc cgatcttttc ggcggccgcc accatgcggc gaatgggacc ggtcatgacg
     1621 gcggccagga gcaccgcccc cagcacgccg aagaccagcg cgatcaaggc cacgtgcagg
     1681 aggcgtttct tcgtctcctg cacggtgcgg cgcaccgagg tttccagggc gtctcgggaa
     1741 aagccgatga cggcggcggc ctgttccggc ccgccggtgt tcaccggata ggccagctcg
     1801 atcacggtac cgcccggcgc cgcggaatct tcccgggaaa ggggcgttcc cgcgtccagc
     1861 gcctgccgga cgaacgggtc gtcgaccgtc cggccgatcc ggtccgagtc ggtgtgcgcg
     1921 tggatcttgc cgcttttctc caccacctgc gcgaacgcca ccccgcgcgc gttccggact
     1981 tctttcatat agctgatgag ggaaacacgg ttgttcgtca gaaaggattc ggcgctgact
     2041 ttctgaaggc tcttggccgt ttcatgcatg cctttcgtca tctcggacag gagatggcgt
     2101 ttttccagat acagcatgga gaacgtggtc cccacgatca ccagggccac caggccgctg
     2161 gaaaaaaggc tgaacttggt ccacaatttc atttcatttc cttccgggcg cggtccagat
     2221 tggtccgcgc ttccgtcaga tccgggtcca gggccagggc gttttcccaa aggcccacgg
     2281 ctttttttag ctggccgttg ccatactcta ccaaggcccg ctcgttgagt tccagggctt
     2341 ttttacggtc ggcggccttc ttttcctgtt cgagccgccg gcggtaggtg agggcgccgt
     2401 cgtggcgttc gtcgaaggag agggccttga tgaagagggc cagagcggcc ggcgcatcgc
     2461 ccgactcgta acacgtcacg ccttcggcaa aatgtttgtc cgctagtttc gcgccgatct
     2521 ccgccgcgcg cgtccgggcc gtctcgctgg acgggtccag cgccaaggcc tgccggcagg
     2581 cgtccagcgc tttctcccaa tggcccttgt cgaactgggc ctgcccgttg cgcaggagaa
     2641 gttccaccgc ctgttgccgg cggatgtctt ccagccgttt cagtttttcg tcgataagac
     2701 ggagttgttc ctgggcatcc gcgcggccgg gccggaaggc cagcacccgt tccatgtgtt
     2761 gccgggcctc gtcgtaccgc ccctcggcat agagagcgac gccgctcttg tagatggatt
     2821 ccgccacctc gtcgtaccgc cgttcgatct ccgcaagcgc gcgggtcgcc tccgcgttgg
     2881 cgggatcgag caagagaacc gccttgaacg cggtggcggc gtcgatgaac atctgccggc
     2941 gatagtagcg ccggcccttt ttcagttgcc cctccacgac tttggctgtt tccgcttgtt
     3001 ccagggtgag ggtgatctgg gccaggaggt cccgcgccgg accgtggccc ggccgcgcgc
     3061 tcagaagctc gttcaggatg cggtaggccc ggagatattc tccccgatgg tagagattgt
     3121 tcgcccggcc gaattgacgg tcgatgtcgt tttcggcctg ggtgtagccg cggtggcggc
     3181 cgtagcggag actgagcgtg aaacaatgcc cgtcctgaaa aacaccccgc ggaacaaagg
     3241 cgtaatcgaa cgagagcccg ctcgtttgca cgccgagccc gtagcggagc ccggagtcca
     3301 ggtcgtccct ctcgcggtag cccacccgca gcgcgatgag gttttgcacc acccactcca
     3361 gccccaggcg ggcgtaacgg tcctcgtcg
//
LOCUS       DAOTFD010000265         3371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21481_length_3371_cov_4.643546, whole genome shotgun sequence.
ACCESSION   DAOTFD010000265 DAOTFD010000000
VERSION     DAOTFD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21481_length_3371_cov_4.643546"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="P5079_10975"
     CDS             <1..327
                     /locus_tag="P5079_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRY30541.1"
                     /translation="IAKCRPPRNRPPKPEESLACRPFLEKQIALLRPTRLVLLGATAL
                     KNLVPTRTKIPMKKAAGRLFPVPALGNAEAVVFYHPAAALYNRKLERVMRRHARQTLK
                     GARGTA"
     gene            348..647
                     /locus_tag="P5079_10980"
     CDS             348..647
                     /locus_tag="P5079_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30542.1"
                     /translation="MLLVLGPTALSLAAPSKKKPAARTTAFEESWDFYKWRIEQGDRL
                     EELENILLRIQRKYAKSSVNMTAVERELAAIRSLLDKKRGGPAGKKGMNHESHRP"
     gene            628..1827
                     /locus_tag="P5079_10985"
     CDS             628..1827
                     /locus_tag="P5079_10985"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRY30543.1"
                     /translation="MKVIVLNCGSSSLKYQLFDMPAEQVLAKGLVERIGVTRAGDAPC
                     FHHQRGDAKWEWNWPMTDHRTAVKKMLEVLTGPETGSLKSIAEIQAVGHRVVHGGPRY
                     TRPVLVDSNVVEDIEVYSMYAPLHNPANALGIRVMREVLPSVPNIAVFDTSFHHTLPP
                     QAFLYGLNGEIAGKYGIRRYGFHGLSHQYVSERAAMFLGKPLSSLRLVTCHIGNGTSI
                     CAVDGGKSVDISMGMTPLEGVIMGTRSGTIDPSIVELLMEKEKLNFQEIIDLLNRKSG
                     LLGLSGVSSDIRDLEKAAREGHRRAQGALDVHTYQIKKYIGSYIFAMNGVDAIIFTAG
                     IGENSSRCRAEICRHLDFLDTWLDEDKNRNCTGEALINTPDSKIKIAVIPTNEEIMIA
                     RATMAWK"
     gene            1947..2429
                     /locus_tag="P5079_10990"
     CDS             1947..2429
                     /locus_tag="P5079_10990"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRY30544.1"
                     /translation="MRKLIACALAVALFPTALMAAGNHPMAGCGLAYILFSKDNNSKA
                     IQILASTTNNLYGTQTFGITSGTSGCTENGTVKFGKEAELYAEINLKELTRDMARGEG
                     EYLGTFAGLLGVQDARRAEFFQLTQQKYGSLVPSAETSSTEMLNALSKEMASHPELLG
                     "
     gene            2511..>3371
                     /locus_tag="P5079_10995"
     CDS             2511..>3371
                     /locus_tag="P5079_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRY30545.1"
                     /translation="MPLISKKTVLAALLLGAAAAVSAADVPALEPAAADYLNELVDQA
                     RQRNLARDISWIRLLHYRRSLLGGWKSQVDGDSFFLSPKGKTQPAGELEATLRAFFQP
                     APADPQTLHPQCQFPARYAWLKSQLRFDPARLPEIRNARFEEWHARIDPGSVTAVFAS
                     SYLNNPASMYGHTFLRVNQAGRKTDEPLTDYCINFAADTPERSGVIFAAKGLLGAYPG
                     RFTTTPYYMKVQQYNNIESRDLWEYTLSLTPEQTDRLARHLWEFGSTYFDYYFLTENC
                     SYQLLPLLEVA"
BASE COUNT          683 a         1221 c          894 g          573 t
ORIGIN      
        1 atcgccaaat gccgcccgcc gcgcaaccgg ccgccgaaac cggaagagtc cctggcctgc
       61 cggccgtttc tggaaaaaca gatcgccctc cttcgaccga cccgcctggt gctcctgggc
      121 gccacggccc tgaaaaacct ggtcccgacg cggacgaaga tccccatgaa aaaagcggcg
      181 ggccgccttt ttccggtccc ggccctgggg aacgccgagg ccgtggtctt ctaccatccg
      241 gccgccgcgc tctacaaccg gaaactggaa cgcgtcatgc gccgacacgc ccggcaaacg
      301 ttaaagggcg cccgaggaac cgcctgaacc gcctcgtccc gctctgtttg ctcctcgtcc
      361 tcggcccaac ggccctctcc ctggccgccc cgtcgaagaa aaaacccgcc gcccgaacaa
      421 cggcgttcga ggagtcctgg gatttctaca aatggcggat cgagcaaggg gaccggctcg
      481 aggaactgga aaacatcctc ctgcggattc agcgcaaata tgctaaaagt tccgtcaaca
      541 tgaccgcggt ggaacgggaa ctggccgcga tacggtccct cctcgacaag aaacgcggag
      601 gcccggcggg taagaaagga atgaaccatg aaagtcatcg tccttaattg cggcagctct
      661 tccctgaaat accagctttt cgacatgccg gccgaacagg tgctggccaa gggactggtg
      721 gaacggatcg gcgtcacccg cgccggcgac gccccctgct tccaccatca gcgcggcgac
      781 gcgaaatggg aatggaactg gcccatgacc gaccatcgca ccgccgtcaa aaaaatgctc
      841 gaggtgctca ccggcccgga aacgggctcc ctgaaatcca tcgccgaaat acaggccgtc
      901 ggccatcgcg tggtccacgg cggcccgcgc tacacccggc ccgtcctggt ggactccaac
      961 gtggtggagg acatcgaggt ctattccatg tacgccccgc tgcacaaccc ggccaacgcg
     1021 ctgggcatcc gcgtcatgcg cgaagtcctg ccgtcggtcc cgaacatcgc ggtgttcgac
     1081 acctcgttcc accacaccct tccgccgcag gccttcctct acggcctcaa cggcgagatc
     1141 gccggaaaat acggcatccg ccgctacggg ttccacggcc tctcccacca gtacgtcagc
     1201 gaacgcgcgg ccatgttcct gggcaaaccg ctctcctccc tccgcctggt cacctgccac
     1261 atcggcaacg gcaccagcat ctgcgccgtc gacgggggga aatccgtcga catctccatg
     1321 ggcatgaccc cgctggaagg cgtcatcatg ggaacccgca gcggcaccat cgacccgtcc
     1381 atcgtggaac tcctgatgga gaaggaaaag ctcaacttcc aggagatcat cgacctcctt
     1441 aataggaaga gcgggctgct gggcctttcc ggggtgagtt ccgacatccg cgacctggaa
     1501 aaggccgcgc gggaaggaca ccggcgggcg cagggcgcgc tggacgtcca tacgtatcag
     1561 atcaaaaaat acatcggctc ctacatcttc gccatgaacg gcgtggacgc catcatcttc
     1621 accgcgggca tcggcgagaa ctcctcccgc tgccgggcgg agatttgccg gcacctggat
     1681 ttcctggaca cctggctcga tgaggacaaa aaccgcaact gcaccggcga ggccctcatc
     1741 aacacgccgg actccaagat caagattgcc gtcatcccca ccaacgagga gatcatgatc
     1801 gcccgggcca ccatggcctg gaaatagggc gcgccgtgaa ccgccgggac ctctcgcccg
     1861 tgctgttttt tatgctttga ccaatctcgc ttatcttgtt atactctccc cagttttcca
     1921 acctaactct tcacacagga ggcattatga ggaagcttat tgcatgcgcg ttggccgttg
     1981 cgctttttcc cacggcgctg atggcggcgg gcaatcatcc gatggcgggc tgcggactgg
     2041 cgtacattct cttctccaag gacaacaact ccaaagccat ccaaatcctg gcatccacca
     2101 cgaacaacct ctacgggaca caaaccttcg gcatcacctc cggcacctcc ggctgcactg
     2161 agaacggcac ggtgaaattc ggcaaggaag cggaactcta cgccgaaatc aacctgaagg
     2221 aactcacccg ggacatggct cgcggcgagg gggaatacct cggcacgttc gccggacttc
     2281 tcggcgtgca ggacgcgcgg cgcgcggaat tcttccagtt gacccaacag aaatacggct
     2341 cgctggttcc gtccgctgaa acctcctcca cggagatgct caacgcgctc tccaaggaga
     2401 tggcgtccca tccggaactg ctcggctaat tcgacctgtt cttcgtacgg gctggcggcc
     2461 ggaaccggtc gccagcccgt attcttttcc cgtccctcca cctcctcccg atgccgttga
     2521 tctctaagaa aaccgtcctc gccgcccttc tcctcggcgc cgccgcggcc gtttcggcgg
     2581 cggacgtccc cgcgttggag ccggccgccg cggactatct caacgaactc gtcgaccagg
     2641 cccgccaacg gaacctcgcc cgcgacatct cctggatccg cctgctccac taccgccggt
     2701 ccctcctcgg cggatggaag agccaggtcg acggcgactc tttcttcctg tcgccgaaag
     2761 gcaagaccca gccggcaggc gaactggagg ccaccctccg cgccttcttc cagccggcgc
     2821 cggccgatcc ccaaaccctg cacccccaat gccagttccc ggcccgctac gcctggctca
     2881 agtcccaact gcggttcgat ccggcccgcc tgccggagat acggaacgcc cgtttcgagg
     2941 aatggcacgc ccgcatcgat cccggctcgg tcaccgccgt cttcgcctcc tcctatctca
     3001 acaaccccgc ctccatgtac ggccacacct tcctccgcgt caaccaagcc ggacggaaaa
     3061 cggacgaacc cctcaccgat tactgcatca acttcgccgc cgacaccccc gaacgcagcg
     3121 gcgtcatctt cgccgccaaa gggcttctcg gcgcctatcc cggccggttc accaccaccc
     3181 cctattacat gaaggtccag caatacaaca acatcgagag ccgcgacctt tgggaataca
     3241 ccctctccct cacgccggaa caaaccgacc gcctcgcgcg gcatctctgg gaattcggaa
     3301 gcacctattt cgactattac ttcctcaccg agaactgctc ctatcagctc ctgcccctcc
     3361 tggaagtcgc c
//
LOCUS       DAOTFD010000266         3370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21488_length_3370_cov_4.571644, whole genome shotgun sequence.
ACCESSION   DAOTFD010000266 DAOTFD010000000
VERSION     DAOTFD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21488_length_3370_cov_4.571644"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..295
                     /locus_tag="P5079_11000"
     CDS             71..295
                     /locus_tag="P5079_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRY30546.1"
                     /translation="MIKVHRLNGTEITVNAELIETVEATPDTGICLVTGNRFVVRESV
                     DEVTEKIVDYRKKISAERKVVNPIEGFRRE"
     gene            354..1130
                     /locus_tag="P5079_11005"
     CDS             354..1130
                     /locus_tag="P5079_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY30547.1"
                     /translation="MDIATILGIILFIGMALRAITWSEGVAGLKPFINVEAFLVVMGG
                     TFCALLVNYDLKQVLGLAKVLKKVLSTTGEDTSQLVTTFVNLSQKAKKEGFLALESDI
                     KNLNDDFLKRALQLVIDGSDQEFIRNMLETEIGFIRERHKVGREIFNALGTYAPAFGI
                     IGTVMGMILMLNSISDVEEVPRRMALALAAAFFGLGAGYLLFLPMAGKLHRRSEEELF
                     VKEIIVRGVLLLQSGATPSLVEANLKAYLEPSKRQLIKET"
     gene            1197..1805
                     /locus_tag="P5079_11010"
     CDS             1197..1805
                     /locus_tag="P5079_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRY30548.1"
                     /translation="MSQIGHPAPPWLVNYADLMTELVCFFVILYAVSAALNKQVVSAK
                     KEIEKMIQEGKVQGEVKVTKEGMYITLQEQGKKVFFESGSAELSPDIKEIVAKIAPTL
                     QNLSQKHDILVEGHTDNVPTTKQYASNWELSTARATNVVRELVQNHGVNPRHISAVGY
                     GEYRPVGTNDSPENRSKNRRVVFFVKNTPPPDVAKQRPPPEE"
     gene            2041..>3370
                     /locus_tag="P5079_11015"
     CDS             2041..>3370
                     /locus_tag="P5079_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRY30549.1"
                     /translation="MGKKYVYFFGNGKAEGKGDQKDLLGGKGAGLAGMSSAGVPVPPG
                     CTITTEVCRHYYANNRKLPKELAPQFKEAMEKLQKSLNKRFGDAKDPLLVSVRSGAKF
                     SMPGMMDTILNLGLNDIAVEGLAAKTANPRFAWDAYRRFIQMFGNVVMEIEKGDFEKV
                     LESVKHEKGVKLDTDLKTDDLKEVVRRYKALVKEKTGQDFPQDPWKQLEQARDAVFKS
                     WNNTRAISYRRLNKIPDDLGTAVNVQAMVFGNMGNDSGTGVGFTRNPSSGEHQFFGEY
                     LTNAQGEDVVAGIRTPKPISELGKDMPEVYKQLRDITTKLEKHYRDMQDFEFTIEKGR
                     LFMLQTRTGKRTGIAAVRLAVEMVTEKLITKEEALLRVEPDQLSQLLAPVFDGKEKAV
                     AVKGGRFLAKGIDAGPGAACGRMAFTAEKAVEMASHGPVVLVRPETNPDDI"
BASE COUNT          783 a         1005 c          987 g          595 t
ORIGIN      
        1 tggtcactta ttgttacttt aatgaaccgg cgcgacaggg tttgagggga aatccccggt
       61 cggttttaaa atgatcaaag tgcaccgtct caacggcacg gaaataaccg tcaacgcgga
      121 attgattgaa accgtggagg ccacgccgga caccggcatc tgcctcgtca cggggaaccg
      181 ttttgtggtg cgggaaagcg tcgacgaggt gacggaaaag atcgtcgatt accgcaagaa
      241 aatcagcgcg gaacgcaaag tcgtcaaccc catcgaagga ttccgacggg aataggccgt
      301 ccgcgccggc cgttcccgaa aacgcgagaa ccgtgcccag gagggcataa cctatggaca
      361 tagcgacgat actcggcatc atccttttca tcggcatggc gcttcgagcc atcacctgga
      421 gcgagggggt ggccgggctg aaaccgttca tcaacgtgga ggcgttcctg gtggtcatgg
      481 ggggcacttt ctgcgctctg ctggtcaatt acgatttgaa acaggtgctg ggtttggcga
      541 aggttctcaa aaaagtcctc tccaccacgg gcgaggacac ctcgcagttg gtcaccacgt
      601 tcgtcaacct cagccagaag gcgaagaagg aggggttcct cgcgctcgag agcgacatca
      661 aaaacctcaa cgacgatttc ttgaaacgtg cgttgcaact cgtcatcgac ggttccgacc
      721 aggagttcat ccgcaacatg ttggagaccg agatcgggtt catccgcgag cggcacaagg
      781 tgggccggga gatcttcaac gcgttgggca cctacgcccc ggcgttcggc atcatcggca
      841 cggtcatggg gatgattctg atgctcaact ccatttccga cgtggaagag gtgccgcgcc
      901 ggatggcctt ggccctggcg gcggccttct tcggcctggg cgcgggctac ctgctctttt
      961 tgcccatggc cggcaagctc catcgtcgat ccgaggaaga actcttcgtg aaggagatca
     1021 tcgtccgcgg tgttctgctc ctgcagagcg gcgccacgcc cagcctggtg gaggccaacc
     1081 tcaaggccta cctcgagccc tccaaacgcc aattgatcaa ggaaacgtga tccggccggg
     1141 cactgtttcc catgccgctt aagccccgaa acctcataga cgaagacgac ccgcgggtgt
     1201 cccagatcgg gcaccccgct ccgccctggc tggtcaatta cgccgacctg atgacggaat
     1261 tggtgtgttt tttcgtcatc ctctacgccg tttccgccgc cctcaacaag caggttgtca
     1321 gcgccaaaaa agagatcgag aaaatgatcc aggaggggaa ggtccagggc gaggtgaagg
     1381 tcaccaagga ggggatgtat atcaccctcc aggagcaggg gaaaaaagtt ttttttgaga
     1441 gcggcagcgc cgaactttcc ccggacatca aggagatcgt cgccaagatc gcccccacgt
     1501 tgcagaacct gagccagaaa cacgacattc ttgtcgaagg acacaccgac aacgtcccca
     1561 ccaccaaaca gtacgcctcc aactgggaac tttccacggc gcgagccacc aatgtggtga
     1621 gggaactggt ccagaaccac ggcgtgaacc cgcgtcacat ctccgccgtg ggatacggcg
     1681 aataccggcc ggtggggacc aacgactcgc cggaaaatag gtccaaaaac cggcgcgtgg
     1741 tcttcttcgt caagaatacc ccgccgccgg acgtcgccaa gcagcggcct cctcccgagg
     1801 agtagtttcg gtttcattcg gttgacgggc ttctggcggt gtgctagact tctaccccaa
     1861 gaggcgcccc catgctgtcg ccgcggggcg ccttccttaa aaagtcacct catcacatag
     1921 tcgggaattt ccaatacccg cagggtcgtg caatagtcag cgatcacggt ttcaaagaac
     1981 acggggacgg tgaagtccgc cccggcgaac tttctaccta ataaggagaa cgaaggacaa
     2041 atgggcaaaa aatacgtgta tttctttggc aacggcaagg cggaaggcaa aggcgatcaa
     2101 aaggatcttt tgggcggcaa aggggccggc ttggcgggca tgtccagcgc cggggtcccc
     2161 gtgccgccgg gctgcaccat caccaccgag gtctgccgcc actattacgc caacaaccga
     2221 aaactcccca aggagctggc cccgcagttc aaggaggcca tggagaaact gcagaaatcc
     2281 ctcaacaagc gcttcggcga cgccaaggac cccttgctgg tctccgtgcg ttccggcgcc
     2341 aaattctcca tgcccggcat gatggacacc atcctgaacc tcggactcaa cgacatcgcc
     2401 gtcgaagggt tggccgcgaa gacggccaat ccccgtttcg cctgggacgc ctaccgccgg
     2461 ttcattcaga tgttcgggaa cgtcgtgatg gagatcgaaa aaggagattt tgaaaaggtc
     2521 ctcgaatcgg tcaagcacga aaaaggcgtg aagttggaca ccgacctgaa gaccgacgac
     2581 ttgaaggaag tggtgaggcg gtacaaagcc ctcgtgaaag agaaaaccgg gcaggacttc
     2641 ccgcaggatc cgtggaaaca gctggaacag gcccgtgacg ccgtgttcaa aagctggaac
     2701 aacacccgcg ccatttccta ccgccggctg aacaagatcc ccgacgatct cggcaccgcc
     2761 gtgaacgttc aggccatggt gttcggcaac atgggcaacg attccggcac cggcgtgggg
     2821 ttcacccgca acccttcctc gggcgaacat cagttcttcg gtgaatacct gaccaacgcc
     2881 caaggcgaag acgtggtggc cggcattcgt acgcccaagc ccatctcgga gttggggaag
     2941 gacatgccgg aggtctacaa acaactccgc gacatcacca ccaaactgga aaaacattac
     3001 cgggacatgc aggatttcga gttcaccatc gaaaaaggcc ggctgttcat gctccagacc
     3061 cgcaccggca aacgcaccgg catcgcggcg gttcgcttgg cggtggagat ggtgacggaa
     3121 aaattgatca cgaaggagga agcccttctg cgcgtggagc cggatcagct ttcccagctc
     3181 ctggcccccg tgttcgacgg aaaagaaaag gccgtcgccg tcaaaggcgg ccgtttcctg
     3241 gccaaaggca tcgacgccgg tcccggcgcc gcctgcggcc gcatggcgtt caccgcggag
     3301 aaggccgtgg agatggcctc ccacggaccc gtggtcctgg tgcgcccgga aaccaacccg
     3361 gacgacatcg
//
LOCUS       DAOTFD010000267         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_21878_length_3329_cov_4.152108, whole genome shotgun sequence.
ACCESSION   DAOTFD010000267 DAOTFD010000000
VERSION     DAOTFD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21878_length_3329_cov_4.152108"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="P5079_11020"
     CDS             <1..1179
                     /locus_tag="P5079_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRY30550.1"
                     /translation="NAYSVSNWTLPAHAALFWGLHPAVLDYWDDEKPTLPPEGVLSIV
                     ELFQKEGYRTGAFVSGDMLGKDFGFERGFDTFVVMVPSSPSATPDMSPALAWIDSSNK
                     PLFLFIHLVINHRPHTPPPPYNLFLDPDQKNLYEGILREIETAANGERSKLYRLSLDR
                     FDFSRNAFSPAFAAALYDGDVRFSDDVAGGLLERIDAMGVGKNSIVVLLSDHGEELNE
                     HGKYFHGRTLYDETIRIPLFIRAPGNDLADKIENRLVSLVDVPPTLLHLAKLPLPKYF
                     QGRPVPPFAPPWDRPIYAQHELEDLSRQAIRTDRWKLILSRGQKPELYDIVEDPAEKN
                     NLAEQPNHRKTLKRLSNDIAAYRQKNLSLRKTLRAPSAPSRPPSDELLKSLRDLGYIR
                     "
     gene            1278..3137
                     /locus_tag="P5079_11025"
     CDS             1278..3137
                     /locus_tag="P5079_11025"
                     /inference="COORDINATES: protein motif:HMM:NF039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HRY30551.1"
                     /translation="MSSPSAVPRTPSIPDAGNDLPLSLGTVKAAFLVVSTLLACLTIL
                     TNVAQTLHFVFKDFLWLAFVACLGSILFLFVHLRSEFRRVTLRDNATLLIVLVTGLMS
                     AALTLVLHRWSPDDYTYLPNVIYHLQNLPSRMGFEIHYVHSGGPPFTSLFYGTSQPYE
                     YAQAVLAYHTRLEPLTVYYLLACPLAGFLMPLALFYAASHFLSSPRLSALGTFIAFLI
                     LPFLIDAPKSFAIQSFARAFQGKTFFLAVGIPLFAAASFDFYRKATRASWTFLAVISI
                     ALVGATASAAVLLPALALILALAHLRFTEKRRFLKSTALYGLSLSYVWGYLWTIHSFA
                     RSDFGPGTWTRSPGFPQTFMEQLVLFFNHAFPLSPLIALASVALSALLLPEGRRSRFL
                     AWVLLPVGLFLNPLTAPFLMDNVYPPSVYYRMFFLFPFPLSIALAAGAVLDRLPTRKN
                     MATVLMAGALLLAHLIFPEHSLLSSTSRWDVGWPKYKVDRPHFTLAKKIAAAAPPGVM
                     LASPAIAGPVTILSARHPQMLHRFEQLKMWLEAREQFQDIQERIDCSWFLDGERGSLE
                     AFKKIVSRYPVLRSIVMRRHPWTLPETQDFCREQGFSDRIDIDDSFVLIWKPS"
     gene            3134..>3329
                     /locus_tag="P5079_11030"
     CDS             3134..>3329
                     /locus_tag="P5079_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30552.1"
                     /translation="MTPSLRDNASCDLLRFHRTVLELLAALLLSFFSAPPARGAAPPA
                     PRRPNVILISVDSLRPDHLGS"
BASE COUNT          581 a         1197 c          794 g          757 t
ORIGIN      
        1 aacgcctatt ccgtctccaa ttggactttg cccgcccatg ccgccctctt ctggggactt
       61 catccggccg tgctggacta ttgggacgat gaaaaaccga ccctgccgcc ggaaggggtc
      121 ctctccatcg tcgaactctt ccaaaaagaa ggctatcgca ccggcgcctt tgtgagcgga
      181 gatatgctcg ggaaagactt cggtttcgaa cgcggcttcg acacgttcgt cgtcatggtc
      241 ccctcgtccc ccagcgccac gcccgacatg tcgccggctc ttgcctggat cgactcctca
      301 aataaacctc tgttcctttt catccacttg gtcatcaatc accggccgca cacaccgcct
      361 cccccataca atttgtttct cgacccggac caaaaaaacc tttacgaagg gatcttgcgg
      421 gagatcgaga cggcggcgaa cggagagcgc tccaaactct accgcctctc gctggaccgt
      481 ttcgacttct cccggaacgc cttctctccg gcttttgccg ccgccctcta tgacggcgac
      541 gtgcgcttct cggacgacgt ggcgggcggc cttctggaac ggatcgacgc catgggcgtc
      601 ggaaagaatt ccatcgtcgt tctattgtcg gaccacgggg aagagctcaa cgagcacgga
      661 aaatacttcc acggaaggac gctctacgac gaaacgattc ggatccctct cttcatccgc
      721 gcccccggga acgacctcgc cgataaaatc gaaaaccgcc tcgtcagcct ggtggatgtc
      781 cctcccaccc tcctccattt ggcgaaactg cctctcccga aatatttcca gggccgcccc
      841 gtgccgccgt ttgccccccc atgggaccgt cctatttacg cgcagcacga actcgaagat
      901 ttgtcccgcc aagcgatccg caccgaccgc tggaaactga tcctgtcccg cgggcagaaa
      961 ccggaactct acgacatcgt cgaagacccg gctgaaaaaa acaacctcgc cgagcaaccg
     1021 aaccatcgga aaacacttaa acgtctctcc aacgacatcg ccgcctaccg gcaaaaaaac
     1081 ctttccttaa gaaaaacgct ccgggcgccc tccgccccaa gccggccccc ctcggacgaa
     1141 ctgctgaaat ccctgcggga cctgggctac atccggtgac cgggcccgag gcgcgttttt
     1201 tcgaccgaag agacggcctg tccgcctcaa cgccggcctc gccgacgcgc agctcatcgg
     1261 ttgggctctg aaccggaatg tcctccccat ccgctgtccc caggacgccg tccatcccag
     1321 acgcggggaa tgacctccct ctcagcctag gcaccgtcaa ggcggcgttc ctcgtcgtct
     1381 ccacgctgtt ggcttgcctg acgatcctca ccaacgttgc gcagacgctg catttcgtct
     1441 tcaaggattt cctctggctg gcctttgtgg cgtgcctggg ctcgatcctg ttcctgttcg
     1501 ttcatcttcg ttcggaattc cgccgcgtca cgctccgaga taacgccacg cttctcatcg
     1561 tgcttgtgac cggactgatg agcgccgcat tgaccctcgt attgcaccga tggagtccgg
     1621 acgactacac ctatttgccg aacgtgattt accatcttca gaacctccct tcacgcatgg
     1681 gcttcgagat ccattacgtc cacagcggag gaccgccatt cacatctctc ttttatggca
     1741 cttcccagcc gtatgaatac gcgcaggcgg tcctcgccta ccacacacgc ctggaaccgt
     1801 tgaccgtcta ttatcttctc gcctgtcccc tggcgggttt tttgatgccc ttggccctgt
     1861 tttatgccgc ttctcatttc ctctcctctc ccaggttgag cgctctggga acatttatcg
     1921 ctttcctcat cctcccgttc ctcatcgacg cgccgaaatc cttcgccatc caatcctttg
     1981 cacgcgcgtt ccaggggaaa acctttttcc tggccgtcgg cattcccctg tttgcggcgg
     2041 ccagcttcga tttttacaga aaggccaccc gggcctcctg gacgtttctt gccgtcatct
     2101 ccatcgccct ggtaggagcc accgcctcag cggccgtcct ccttcccgcg ttggcgctga
     2161 tcctggccct ggcccatctg agattcaccg aaaaacgccg attcctcaaa tccacagcgc
     2221 tctacggtct ctccctttcc tatgtctggg gatatctgtg gaccatccac tcttttgcac
     2281 ggtcggattt cggtccgggc acctggacgc gctcgccggg attccctcaa acgttcatgg
     2341 aacaattggt cctgttcttc aaccacgctt ttccgctctc tcctctcatc gccctcgcct
     2401 ccgtcgccct gagcgccctc ctcctgccgg aaggccgacg gagccgattc ctggcctggg
     2461 ttctcctgcc cgtcggcctt ttcttgaacc ccctgaccgc gccgttcctc atggacaacg
     2521 tttatccgcc gtccgtttac tacaggatgt ttttcctctt tccttttcct ctctccatcg
     2581 ccctggccgc cggggcggtc cttgatcgcc tgccgacgcg caaaaacatg gccaccgtcc
     2641 tcatggccgg cgccctgctt cttgcgcatc tcattttccc ggagcactcc ctattatcgt
     2701 ccacgtcccg ctgggacgtc ggctggccga aatacaaggt cgaccgcccc catttcacgc
     2761 ttgccaaaaa gatcgcggcg gccgccccgc cgggcgtcat gctggcctcc ccggccatcg
     2821 cgggccccgt cacgatcctc agcgcccgcc atccgcagat gcttcaccgg ttcgaacagc
     2881 tcaaaatgtg gctggaggcg cgcgaacaat ttcaggacat ccaagaacga atcgactgtt
     2941 cctggttcct cgacggcgaa cgcgggagcc tggaagcgtt caaaaaaatc gtttcccgct
     3001 atcccgtcct acgctccatt gtgatgcggc gtcacccctg gactcttccg gagacgcagg
     3061 atttttgccg ggaacaaggt ttttccgacc gtatcgacat cgacgactct tttgtattga
     3121 tatggaaacc ctcatgaccc cttcactccg ggacaacgcc tcctgcgacc ttctccgttt
     3181 tcatcgaacg gttctcgagc tgctggcggc gctcctgctt tccttctttt ccgcgcctcc
     3241 ggcccgcgga gccgccccgc ctgccccacg ccgtcccaac gtcatcctca tctccgtcga
     3301 ttcgttgcgc ccggaccatc tcgggagtc
//
LOCUS       DAOTFD010000268         3306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_22112_length_3306_cov_3.646570, whole genome shotgun sequence.
ACCESSION   DAOTFD010000268 DAOTFD010000000
VERSION     DAOTFD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22112_length_3306_cov_3.646570"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>3306)
                     /locus_tag="P5079_11035"
     CDS             complement(51..>3306)
                     /locus_tag="P5079_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRY30553.1"
                     /translation="PGDDGGLRDIVNGRVRLDYSTDGGYAFSTSTSKIELSTSVSPGQ
                     AVALTVTGLTGGSTYYFRVWTLDEALNSAGLSNGVSTYAQVDVTPPAAITDVSAAASW
                     RRVALSWTAPGDDGGAGVLAGNVRIRYSVTAPITNESEFDSAPFEVLLATTVSPGQSV
                     LHVVTDLADGTPYYFALRAEDNLGNPGTIVARSTASAVPVNAVPGAFALASPPDGAIS
                     ASASVTLDWNNAVDADTVLGDSVTYTLEYSTDASFTLAVTTFSGLAVSSQAVTLSALL
                     ENLTTYWRVRAKDGDGAETSATPASFKLYLNQDNTAPTAFNLVSPADAGNVPTKQPLL
                     DWEDAVDIDPGDVVTYRVDYTTVPGFGTYASSASLPASQLVVPAPLLEDSTYYWRAYA
                     TDGVTPVLCNQGSYSFQVDATSSPPASFGLISPAQGVRSTTTTVTFSWQPTTDPDPKD
                     SVSYNLVYSILQNFGSSTTVSGLALTSHTVTFSDNSPVYWFVEAQDSGGNKRTSSESL
                     RYLTIDTAKEIPLPFELLFPTGSVIVDTLKPFLQWASAVDPDPLEQVRYSLDLSDRAD
                     FVGAQGIPLTDNFYQVTNNLLDDTTYYWRVRAGGYRGVPPVLQDAEERQSDVGVFVVK
                     VVNSNPQNFALLSPANGSVVSTTRPVFRWQAAMDVDFNDIVRYTLVISSVADFSTSSA
                     YVDISTPVFEMSAPLLEKRNYYWKVVAKDKEGAETVCGETFRFFLPDLTRPRPPLGLK
                     GTWSAPKTAYIVQWNAVTANTDGSAVSDLKGYRVYRSLFAETLGDSTFVAELPVTTLS
                     YEDAAVNGGDFFYAVRAVDDSGIESANSLLVESRSTPMFVALSDDREVQVHIESLRYQ
                     GGASGTTVSEDAVVVQLNRRSAEEVGSVLRSYELEIKEATSDQKIDRFRFQKPVEVAF
                     SVNPPAAAAPSPSISGRPVIATAPSLPAEQMSIFWYNGVEYVKLGGYYDSDKKTISVM
                     SQWPGVYRLQQAFQATSFEVVQLWPRKVFTPNGDGINDEMNFVYSNPKLVPVSGKIFD
                     LYGAHVADLQAGRDNSSLIWDGRDSDGHFVAKGVYCYQITSDGKTLNGTIVVAR"
BASE COUNT          638 a         1031 c         1075 g          562 t
ORIGIN      
        1 gtcccgccag aaggaggcat cggattgtcc gcgtccgtcg tgtcttcata tcatctcgcc
       61 accacgatcg tgccgttcag cgtttttccg tcgctggtga tctgatagca gtagacccct
      121 ttggcgacaa aatggccgtc ggagtcgcga ccgtcccata tcagagagct gttgtcgcgg
      181 ccggcctgga ggtcggccac gtgggcgccg tagagatcga aaatctttcc gctgaccggg
      241 acgagcttcg ggttgctgta gacgaagttc atctcgtcat tgatcccgtc gccgttcgga
      301 gtgaacactt tgcgcggcca gagctggacc acctcgaacg aagtggcctg aaaagcctgt
      361 tggagccggt agacgccggg ccattgcgac atgacggaga tggtcttctt gtccgaatcg
      421 taatacccgc ccaacttgac gtattcgacc ccgttgtacc aaaagatgct catctgttcc
      481 gccggcagcg acggggccgt ggcgatcacg ggccggccgg aaatcgacgg cgatggggcg
      541 gccgccgcgg gcggattgac ggagaacgcc acctccaccg gtttctggaa ccggaaccgg
      601 tctatcttct ggtccgacgt cgcctccttg atttccaact cgtaggaccg caacaccgag
      661 ccgacctcct cagccgaacg acggttgagt tgaaccacga cggcatcttc cgagacggtc
      721 gtcccggaag cccctccctg gtaacggaga gactcgatgt ggacctggac ctcccggtcg
      781 tcggacaggg ccacgaacat cggggtggac ctcgattcga ccaacaacga attggcgctt
      841 tcgatgccag aatcgtccac cgcccgcacc gcgtagaaaa agtccccgcc gttcacggcg
      901 gcgtcctcgt aggagagcgt ggtgacgggc aactccgcca cgaacgtcga atcccccagg
      961 gtctcggcga aaagagaccg gtagacccga taccccttca gatccgagac ggcgctcccg
     1021 tccgtattgg cggtgaccgc gttccactgg acgatgtagg ccgtcttggg cgcggaccaa
     1081 gtccccttga gaccgagcgg gggcctgggc cgggtgagat cgggaaggaa gaaccggaac
     1141 gtctccccgc acacggtttc ggcgccctcc ttgtccttgg cgacgacttt ccagtaataa
     1201 ttgcgcttct cgagcagcgg cgcggacatt tcaaaaaccg gcgtgctgat gtccacgtag
     1261 gcggaactcg tcgagaaatc cgcgacgctg gagatgacca gcgtatagcg gacgatgtcg
     1321 ttgaaatcca catccatggc cgcctgccag cggaagacgg ggcgggtggt gctcaccacg
     1381 gaaccgttgg cggggctcag cagggcgaaa ttctgcgggt tgctgttgac gaccttcacg
     1441 acgaacaccc ccacgtccga ctgccgttcc tccgcgtcct gcaggacggg aggaaccccg
     1501 cgatagcccc ccgcccggac gcgccaataa tacgtggtgt cgtccaagag gttgttggtg
     1561 acctgataaa aattgtccgt caacgggatc ccctgcgccc cgacgaaatc cgcccgatcg
     1621 gagaggtcca gcgaataacg gacctgttcc aacgggtccg gatcgaccgc cgaggcccac
     1681 tgcaggaacg gcttgagcgt atcgacgatg acgctcccgg tcggaaacaa cagctcgaac
     1741 ggcaacggga tctccttggc ggtgtcgatg gtgaggtatc ggagcgattc ggaggaggtc
     1801 ctcttgttgc cgccggagtc ctgggcttcc acgaaccaat acaccgggga attgtcgctg
     1861 aaggtcaccg tgtggctggt gagggccagg ccggagaccg tcgtgctcga accgaaattc
     1921 tgcaggatgg aatagaccag gttgtaggac accgagtcct tggggtccgg gtcggtggtg
     1981 ggctgccagg aaaaggtgac cgtcgtggtc gttgaacgga cgccctgggc gggcgaaatc
     2041 agcccgaaac tggcgggagg ggacgacgtg gcgtccacct ggaacgagta actcccctgg
     2101 ttgcagagga cgggcgtgac gccgtccgtg gcgtaagcgc gccaatagta ggtgctgtct
     2161 tccaacaacg gcgccggcac aacgagctgg gacgccggca aagacgcgct ggaagcgtac
     2221 gtgccgaacc cggggacggt cgtgtaatcg acccggtagg tgacgacgtc gccgggatcg
     2281 atatcgacgg cgtcctccca gtccaacaag ggctgtttcg tcggcacatt gcccgcatcc
     2341 gccggcgaca ccaggttgaa cgccgtgggg gccgtgttgt cctggttcaa atacaattta
     2401 aaagacgccg gggtggccga cgtctcggcg ccgtccccat ctttcgcccg cacccgccaa
     2461 tacgtcgtca ggttctccag aagcgcggaa agggtgacgg cctgggaaga gacggccagg
     2521 ccggagaacg tcgtcacggc gagcgtaaac gaggcgtccg tcgaatattc cagcgtgtag
     2581 gtgacgctgt cccccagcac ggtgtccgca tcgacggcgt tgttccaatc cagcgtgacg
     2641 gaggccgacg ccgaaatcgc gccgtcggga ggcgaggcca gggcgaacgc gccgggcacg
     2701 gcgttgacgg gaacggccga cgcggtcgag cgcgccacga tcgttcccgg gttccccaaa
     2761 ttatcctcgg cgcggagggc gaaataatag ggggtgccgt cggccaaatc ggtcacgaca
     2821 tgcaaaacgg actggccggg agaaaccgtg gtggcgagga ggacttcgaa cggggcggag
     2881 tcgaattcgc tctcgttggt gatgggagcg gtcacgctgt agcggatccg cacgttcccc
     2941 gccaggacgc ccgccccgcc gtcgtcgccg ggcgcggtcc aggagagggc cacccgccgc
     3001 caggacgccg cggccgacac atccgtgatg gccgccggcg gcgtcacgtc cacctgcgcg
     3061 tacgtcgaaa ccccgttcga cagccccgcc gaattcaacg cctcgtccag cgtccaaacg
     3121 cggaaatagt aggtgctccc gccggtgagg cccgtcaccg tcaaagcgac cgcctggccg
     3181 ggcgacaccg acgtgctcaa ctcgatcttc gaggtgctcg tggagaaggc gtatcctccg
     3241 tcggtgctgt aatccaggcg cacccgcccg ttcacaatat cccggaggcc tccatcgtcg
     3301 ccgggg
//
LOCUS       DAOTFD010000269         3293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_22259_length_3293_cov_3.616430, whole genome shotgun sequence.
ACCESSION   DAOTFD010000269 DAOTFD010000000
VERSION     DAOTFD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22259_length_3293_cov_3.616430"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="P5079_11040"
     CDS             complement(<1..342)
                     /locus_tag="P5079_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY30554.1"
                     /translation="MPLSPMLFEPLKRSADSLGLPAFLKSLEKELSAFSRAHEGFLSR
                     LTDYVLTGEGKRLRPALVFLSAQFGKPSSKDVLRISLAVEMIHIATLIHDDIVDQAAV
                     RRHRSTVAAKFG"
     gene            complement(342..1772)
                     /locus_tag="P5079_11045"
     CDS             complement(342..1772)
                     /locus_tag="P5079_11045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRY30555.1"
                     /translation="MGHLSALSPELFLTIAALGLLLADAVWPGRRKTSVAVAIGAVLA
                     AAVLLFKCGGDGVVLGMMAADGMGRFFKLILLGGMALVLWMSLDYRAFRSSSADREET
                     VSETAWGTYAALLLLATVGLLLLVSAVDFLLIVVALELIGVTSFILTGFLRHDRRSNE
                     AAIKYFLVGAFSSALMIYGVSLLYGITGSTAIRAVLEADLGVASRFPLIAAVLFILVG
                     FGFKLAMVPFHMWVPDAYEGAPTPITAFLSVAPKAAAAGILVRTFAAHGELGLSSLLA
                     VLAAVTMTVGNLAALRQSNVKRLLAYSSIAQMGYVLVGFVAAGKLGILSVLVYSAAYL
                     VMNLGAFACVIAVSNDAGMDDLMGFSGLSRRSLPLALATTVFMLSLTGIPPMVGFVGK
                     FSVFAAAVAEGWVWLAVVGVINSVISLYYYFGIVHRMFFVDSERPLRISVAASLVGCI
                     SVTLLGTLAAGLFPGPLLEWVRRVVL"
     gene            complement(1775..3268)
                     /locus_tag="P5079_11050"
     CDS             complement(1775..3268)
                     /locus_tag="P5079_11050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRY30556.1"
                     /translation="MGLLSLIVFVPLIGALSMLFLKDDARGRDSARWLATAFSAVPLV
                     LSGWLYFDFDTASPAMQYLEKLRWIPAFNIHYSVGVDGLSLPLVILTALLSFVAVVAS
                     FGIEHRVREYFFWFLALECGMLGVFVAQDFFLFYVFWEVTLVPMYFLIGIWGGPKKEY
                     AAIKFFLYTLFGSVFLLLGMLALYFAGEIRTFDFVELAQQSRGMAMGMQILIFLALYV
                     GFAVKIPAFPFHTWLPLAHVEAPTAVSVILAGVLLKMGVYGLLRVSFPILPQATQWFI
                     YPMTVIAFINIVYGAFCAMAQKDMKRMVAYSSINHMGYCLLGMAALTGVNAAAGLSGA
                     VLQMVTHGIITGSLFLLVGVLYDRAHTRDIDQFGGLGAKLPVYTGIMTVQVMASLGLP
                     GLAGFVSEFLCFLGAFEQASLRLWVALSVIGILITAAFFLKLIKDVFLGEFNAKWEGV
                     LSDMTPREIFATAPLLLLTFLIGVYPRAALRLMDVTMNDLIRRVLGG"
BASE COUNT          836 a         1073 c          964 g          420 t
ORIGIN      
        1 cccgaatttc gccgccaccg tggaacggtg ccggcgcacg gccgcctggt ccacgatgtc
       61 gtcgtggatc aaagtggcga tgtggatcat ctccaccgcc agagaaatcc tcaagacgtc
      121 cttcgacgag ggcttgccga attgagccga caggaagacc aacgccggac gaagccgttt
      181 cccctcgccg gtcagcacat aatccgtcaa gcgggataaa aaaccctcat gcgcccggga
      241 aaaagccgac agctccttct ccaatgattt caggaacgcc ggcaaaccga gcgaatccgc
      301 ggaacgtttc agcggctcga acagcatcgg ggagagcggc atcaaagcac cacccgccgg
      361 acccattcca ggagggggcc cgggaacaga ccggccgcaa gggtccccag gagcgtcacg
      421 gagatgcagc cgaccaacga cgccgccacg gagatccgca acggacgctc ggaatccacg
      481 aaaaacatcc ggtggacgat gccgaaatag taataaagcg agatcacgct gttgatgacc
      541 cccaccacgg ccagccagac ccagccctcg gccacggcgg cggcaaagac ggagaatttc
      601 ccgacgaacc cgaccatcgg cgggatgccc gtaagagaga gcatgaaaac cgtggtggcc
      661 aacgccagcg gcagggaccg ccgggagagg ccggaaaatc ccatcaggtc gtccatgccg
      721 gcatcgtttg aaacggcgat cacgcaggca aaagcgccca ggttcatgac cagataggcc
      781 gccgaataga ccaacacgga caaaatcccc agtttccccg ccgcgacgaa tccgaccagc
      841 acatacccca tctgcgcgat ggaggaatac gccagcagcc gtttgacgtt gctctgccgc
      901 aacgccgcca ggttccccac cgtcatggtc acggcggcca ggacggccag cagggaggag
      961 aggccgagtt ccccgtgggc ggcgaacgtc cgaaccagga tccccgcggc ggccgccttc
     1021 ggcgccaccg aaaggaacgc ggtgatgggc gtgggcgcgc cctcgtacgc gtcgggcacc
     1081 cacatatgga acggaaccat ggcgagcttg aatccgaaac cgacgaggat gaacaggacc
     1141 gccgcgatga gcgggaaacg cgaggcgacg ccgagatcgg cctccagcac cgcccgaatg
     1201 gcggtactgc ccgtgatgcc gtagagcagg gacacgccgt agatcatcag ggccgacgaa
     1261 aaagccccca ccaggaaata tttgatggcg gcctcattgg aacgccgatc gtgccgcaga
     1321 aaaccggtca ggatgaagga ggtgaccccg atcaactcca acgccaccac gatgagcaga
     1381 aaatccacgg cggacaccaa cagcaacagc ccgaccgtcg ccaggaggag caacgccgcg
     1441 taggtccccc aggccgtctc ggaaacggtt tcctcccggt cggccgagga gctccggaag
     1501 gcccggtaat ccagggacat ccacaacacc aaggccatgc cccccaagag gatcaatttg
     1561 aagaagcggc ccatcccatc ggcggccatc atgcccaaca ccaccccgtc gccgccgcat
     1621 ttgaacaaga gaacggccgc cgccagaacc gcgccgatcg ccacggccac ggaggtcttc
     1681 cgccggccgg gccagacggc gtcggcgaga aggagtccca acgccgcaat ggtcaaaaag
     1741 agttcgggcg acaacgccga cagatggccc aaggtcatcc ccccaacacg cgccggatga
     1801 ggtcgttcat ggtgacgtcc atcaagcgca gcgccgcgcg gggataaacg ccgatgagga
     1861 acgtcagcag caggagcggc gcggtggcga agatctcacg cggcgtcatg tcgctgagga
     1921 ctccctccca cttggcgttg aattccccca gaaaaacgtc cttgatgagc ttcagaaaga
     1981 acgccgcggt gatgaggata ccgatcaccg aaagcgccac ccacagccgc agagacgcct
     2041 gttcgaaggc gccgaggaaa cagaggaact ccgacacaaa ccccgccaac cccggcaggc
     2101 cgagcgaagc cattacctgc acggtcatga tgcccgtgta gaccggcagt ttcgcgccga
     2161 gcccgccgaa ctggtcgatg tcgcgcgtgt gggcgcggtc gtagagaacc cccaccaaaa
     2221 ggaacaggga gcccgtgatg atgccgtgcg tcaccatttg gagaaccgcc cccgaaagac
     2281 ccgccgccgc gttgacgccc gtcagggccg ccattccgag caggcaataa cccatgtggt
     2341 tgatggacga ataggccacc atgcgtttca tgtccttctg ggccatggcg cagaacgcgc
     2401 cgtacacgat gttaatgaag gcgatgaccg tcatcggata gatgaaccat tgcgtggcct
     2461 gcggcaggat ggggaacgac acccgcaaga gaccgtacac ccccattttc aacaacaccc
     2521 cggccaggat caccgaaacc gccgtggggg cttccacgtg cgccaacgga agccaggtat
     2581 gaaacgggaa cgcggggatc ttgacggcaa aaccgacgta gagcgccagg aagatgagga
     2641 tctgcatccc catcgccatg ccgcgggact gctgggccag ttcgacgaaa tcaaacgtcc
     2701 ttatctcccc agcgaaatag agcgccagca tccccaacaa caggaacaca ctgccgaaca
     2761 acgtgtagag gaagaacttg atggcggcgt attccttttt cgggccgccc cagatgccga
     2821 tgaggaaata catgggcacc agcgtcactt cccagaacac ataaaaaagg aagaagtcct
     2881 gcgccacgaa cacgcccagc atgccgcact cgagcgccag gaaccagaaa aaatattccc
     2941 tgacccggtg ctcgatgccg aaggacgcga ccaccgcgac gaaagacagc aacgcggtca
     3001 gtatgaccaa cggcaacgaa aggccgtcca cccccaccga atagtgaatg ttgaacgccg
     3061 ggatccagcg caatttctca aggtattgca tggccggcga ggcggtgtcg aaatcgaaat
     3121 aaagccaccc ggacaacacc agcggcaccg ccgaaaaagc ggtggccagc cagcgcgccg
     3181 aatccctccc ccgggcgtcg tccttcagga aaagcatcga gagcgcgccg atgagcggaa
     3241 cgaaaacgat cagactcaga agacccataa tcaaagctcc ttacacgtta tcg
//
LOCUS       DAOTFD010000270         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_22324_length_3287_cov_3.492884, whole genome shotgun sequence.
ACCESSION   DAOTFD010000270 DAOTFD010000000
VERSION     DAOTFD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22324_length_3287_cov_3.492884"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..>3287
                     /locus_tag="P5079_11055"
     CDS             168..>3287
                     /locus_tag="P5079_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30557.1"
                     /translation="MNAETPKHIQLLGRKLFLRPLPYTPHLRRYLPSGRFLKRTASLT
                     MSVVVFYSQVLCAGTSFLPTVSMGLPFGRTTETLMKRLEKWTDQGRDVLGYVFHGSVY
                     EDRNKLLEPGSTPETLRRQEDRQKANMDMQQMNMQNRGLQLPSLLKQPGQETSQDTLK
                     KIKLLDEGPLPGEEVSDAIQKEIEMLDQKMKKLASEGADFKYQVKLNDTGTLQKMYFK
                     DGQLHRIVNYEYYDTHGHIIRENHETTYDSSDLLKSRNIERFDAHGERSVTYQEYKYQ
                     RGARFYDSKTQLISEIDEKITTPTGSVSRRVQKDMVYDGKRNLVSFNEKKTDEKGVES
                     ERWFWDAKYDDDKNILSYKQLSRENGADTLVNWSEGTYVKNPQWKERKPGEGDKYGHQ
                     QFLLAGYKQEIRSGDGSFQTQVWSNMAYNQYDNMVSYDRRTTDFQKLEIVTQFRDGVY
                     DEFDRMTSYNQDTWDSFRHHQRLEFTGATYNNFDDVLSYVERVTEEGKTRERAFSGGI
                     YDAAHNLMASYEKITDSAGQALVKDWSASGYEKGQLTGYHEVLTYDDMRADTTVTEIG
                     YDSLGRPSERYEVKHVWGTGSDGRAVDVKILTDIQRDSEELQEEEVVLDAGLGIVMKM
                     RNVYGYGETKELQAWDAAGRLVRDLTEFTTRSNMTAKGDYYEKKRTVGQDALGGTVLD
                     STAETDRTAGLYDGFGRLMDYSEVTIQNGLAGTRSKRTVDGMIYNVDGNLVVSREVSQ
                     NSDGAVTVSNRSEVEYDSLGRMTRYEVDTFSEAEGIRQVTHVERENIAYNGLDDISGY
                     NERILEEGSDVLREVSWQGEYNRFAQLDHSRQTTHRYGTNLDGETLDVTDVTSTYGMK
                     YSLNGLLAGYTEEMVSDATPALMTINVLDGISYDDAGRMIGFTQTTHQVSRSAVTPGG
                     SSRNFFIDESAAALHSVAVTRRLGATYNPLGLVTGYVDVVGENGLTTTNLVSDIEYNS
                     RGQMTGSTTISHKTGQEPQTFYYRADDPDQAPLSAWELTALLNQYPGKSMEELLSLRD
                     ENGNPSVLVA"
BASE COUNT          805 a          893 c          974 g          615 t
ORIGIN      
        1 cgccctggct tctcgaatag cctgaaaatc cttgtttcct tccggcggcc tgttttttgt
       61 tactaaaagt ttaacgcttg tttaccacca ttttaccgat attttccacg ccttttacaa
      121 ttccttaagg caaaagctga cctttttgct tatagttgtc tatagtcatg aacgccgaga
      181 cccccaaaca cattcagctt ttgggcagaa agctttttct gcgtcccctg ccgtataccc
      241 cccacctccg tcgatatttg ccctccgggc ggttcctgaa acgaacggcc agcttgacga
      301 tgtccgtggt ggtgttttat tcgcaggtcc tctgcgcggg caccagtttc ttgccgacgg
      361 tgtcgatggg acttcccttc ggccggacca ccgaaacgct catgaagcgc ctcgaaaaat
      421 ggacggatca gggacgggac gtcctcgggt atgtttttca cggcagcgtc tacgaagatc
      481 gaaacaaact tcttgagccg gggagcacgc cggagacgct gcggagacag gaagaccgcc
      541 agaaggcgaa catggatatg cagcagatga acatgcagaa ccgcgggctc caacttccct
      601 cacttctgaa acagcccggc caggaaacct cgcaggatac cttgaagaag atcaaactgt
      661 tggacgaagg acccctcccg ggggaggaag tgagcgatgc catccagaaa gaaatcgaaa
      721 tgctggacca gaaaatgaaa aagttggcca gcgaaggggc ggattttaaa taccaggtca
      781 aattgaacga caccggcacc ctgcagaaga tgtattttaa ggacggacag ctccatcgca
      841 tcgtcaatta tgagtattac gacacgcacg ggcatatcat tcgggaaaac cacgaaacca
      901 cctacgactc ctccgacctt ctgaaatccc gcaacatcga gcggttcgac gcccacgggg
      961 aacgttcggt cacctatcag gaatataaat accaacgcgg ggcccgtttc tacgattcca
     1021 agacacaatt gatatcggag atcgatgaaa aaatcaccac ccccaccggg tcggtgagcc
     1081 gccgcgtcca gaaggacatg gtgtacgacg gcaaacggaa cctggtgagc ttcaacgaga
     1141 aaaagaccga cgaaaaggga gtggaatcgg agcgctggtt ctgggacgcc aaatatgacg
     1201 acgataagaa cattctttcc tataagcaac tgtcgagaga gaacggcgcg gacacgttgg
     1261 tgaattggtc ggaggggacc tacgtgaaaa atccacagtg gaaggagagg aagcccgggg
     1321 agggggacaa atacgggcat cagcagtttt tgctggccgg ctataagcag gaaatccgtt
     1381 ccggcgacgg ctccttccag acgcaggtgt ggagcaacat ggcttataac cagtacgaca
     1441 atatggtgtc ctatgaccgc cgcaccacgg attttcaaaa actggagatc gtcacgcagt
     1501 tccgcgacgg ggtctacgat gaattcgacc ggatgacgtc ctacaaccag gacacctggg
     1561 attctttccg gcatcatcag cgtttggagt tcacgggcgc tacctataat aattttgacg
     1621 acgtcctgtc ctacgtggaa cgcgtcacgg aagaagggaa aacccgggaa cgggcgttca
     1681 gcggcgggat ctatgacgcg gcgcataacc tgatggcgtc ctatgaaaaa ataacggaca
     1741 gcgcggggca ggccctcgtg aaggattgga gcgcctccgg ttacgaaaag ggccaattga
     1801 cgggctatca cgaagtgctc acctacgacg atatgcgtgc ggacaccacc gtcacggaga
     1861 tcgggtatga ctctttgggc cggcccagcg aacggtatga ggtgaagcac gtttggggga
     1921 ccggaagcga cggccgggcg gtggacgtca aaatattgac ggacatccag cgtgactcgg
     1981 aggaacttca ggaggaggag gtcgtcttgg acgcggggct ggggatcgtc atgaaaatgc
     2041 ggaacgtcta cggctatggg gaaacaaagg agcttcaggc gtgggacgcc gcggggaggc
     2101 tcgtgcggga ccttaccgaa tttacgaccc gctccaacat gaccgccaag ggggactatt
     2161 acgagaaaaa acgaaccgtc ggacaggacg ccctcggcgg caccgtgttg gattcgaccg
     2221 ctgaaaccga tcggaccgcc ggtttgtatg acgggttcgg acggctcatg gattacagcg
     2281 aggtgaccat ccagaacggc ctcgcgggca cgcgcagcaa aagaaccgtg gacggaatga
     2341 tctacaacgt ggacggcaac ctggtcgtca gccgcgaggt cagtcagaac agcgacggcg
     2401 cggtgacggt ttccaaccgt tcggaggtgg aatacgattc gttgggccgg atgacccgct
     2461 atgaggtgga cacgttctcc gaagcggagg ggatccggca ggtcacccac gtggagaggg
     2521 aaaatatcgc ctataacggg ctggacgaca tttccggtta taacgagcgg atactcgagg
     2581 aggggagcga cgtgctgcgg gaggtgagct ggcagggcga atacaaccgc ttcgcgcaac
     2641 tcgaccattc ccggcagacc acccaccgct atgggaccaa cctcgacggc gaaaccctgg
     2701 acgtgacgga cgtcacctcc acgtatggga tgaagtattc gttgaatggc cttttggccg
     2761 gatacaccga agagatggtg agcgacgcca cccccgccct gatgaccatc aatgtgttgg
     2821 acggcatctc ctacgatgac gccgggcgga tgatcggttt cacgcagacc acgcatcagg
     2881 tctctcggtc ggcggtgacc ccgggcggtt cctcccggaa ttttttcatc gatgaatcgg
     2941 ccgcggcgct gcattcggtg gcggtgaccc ggcggctggg cgccacctac aaccctctcg
     3001 gtcttgtgac gggctacgtg gacgtcgtgg gcgagaacgg tttgaccacc accaacctcg
     3061 tttcggacat cgaatataac tcccgcgggc agatgaccgg ctccaccacc atcagccaca
     3121 aaaccgggca ggaaccccag acgttctatt accgggcgga cgatccggat caggcgcccc
     3181 tcagcgcctg ggaattgacc gccctgctga accagtatcc cggcaaaagc atggaggaat
     3241 tgctgagctt gagggatgaa aacgggaacc cctcggtgct ggtggcc
//
LOCUS       DAOTFD010000271         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_23429_length_3184_cov_4.502717, whole genome shotgun sequence.
ACCESSION   DAOTFD010000271 DAOTFD010000000
VERSION     DAOTFD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23429_length_3184_cov_4.502717"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3184
                     /locus_tag="P5079_11060"
     CDS             <1..>3184
                     /locus_tag="P5079_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30558.1"
                     /translation="KADFTVNYGLDLLAQKLFAVANREQVPEVYAEFRSAWEKTPGVR
                     KSLGALDCLPVLLTGTQGDRYLVALVAGPEGRVPVVYREGQDGVYKQGNDAVRELLFS
                     TRWTNKAWEMQLAHRQFAEWGLTDEKPFFHPRRLIEGVQGTLAAQQFVEKVLEEKPFV
                     RNPAAAEQEALTSSLAALENADAATRARGVAELTRFLTGPNPDLSTLKSLVAVLENDS
                     PAVAEVRQILQTAGEPAIPLLTALMGNAQKSAAARARAAETITPILRTLRGRQSYARF
                     QKTLSTNKVLEALSVSESEKRSEMAAALAELRTVLPGKAERARLVAAKTLWVAGGAIT
                     LVAVIAGKASPLSLAALGIAFAAQRLFPKNTLLQRVLPLAFVLVSAAALGGLLHVLPF
                     LALSLITYQAMRWMRRAPRWARLAFLVPALFLVSSGSAAAAELVKDGQAWLLGGQKFD
                     WISFRAQADLKQAGLINTDTSAADAQRLIAERVDEYTELMRAKVDPNWNPNNVHEGDI
                     VLRPPLGETVPEAEVTVTPVSETPAVVRESSPQTESTAVREEASLAPQADSALSAVSD
                     VLLPALLVLAAVSVLVFFLYRAYRRRLNQLADPISFSRYLFRDLPGRLWAKVRPAEQA
                     AETVEEAKTAETASSLSLQPTPPAPTDVEAPVEKEAPAPGTQMELFRPEELPPATPVA
                     APKPADVKTAAADKSKKGAEAVQLLLDFEQQQQQQTAAGPKAEEKTLTAVNINELSIE
                     QLAALLKEAGVRLPYSRVATNIFNHVRSKGPLLSAEALIDAHLGIGQKSWERLAKYFI
                     LTAPEKPAEPAADQAAPAKSIGPDTTVPSQDEPTPASLVDNLAETAQDVARDVLPDVL
                     ERPLRTVTDAVLPSADETSAAEEPAAEEPTPPQKMDQAAEPLDINKASVEQLAALLKK
                     TGAVIAYNKVAERIAARIKEKGPLGSAQELLDAHVGVGQKAWEKLAQSLTFGKEGTGG
                     EAADQEPADTDRAEEPSPIEGMARAAKETVDGFMPTLPQTAPAEATAEEKPSSEQSAT
                     KTTKTRKPKTTKKDAPAAVSESSPIAAEMPV"
BASE COUNT          642 a         1123 c          951 g          468 t
ORIGIN      
        1 aaagcggact tcaccgtcaa ttacggcctg gacctcctgg cccaaaaact tttcgccgtg
       61 gccaaccgag aacaggtgcc ggaagtgtat gcggaattcc gctcggcctg ggagaagacc
      121 ccgggcgtcc gaaaatccct gggcgcgctc gattgcctgc ccgtgctcct caccgggacg
      181 cagggggacc gttacctggt ggccctggtg gcgggcccgg aaggccgcgt gccggtggtc
      241 taccgcgaag ggcaggacgg ggtctacaaa caaggcaacg acgcggtacg ggaactcctt
      301 ttctccacac gctggacgaa caaggcgtgg gagatgcagc tggctcaccg ccaattcgcc
      361 gaatggggcc tgaccgacga aaaacctttc ttccatcccc gccggctgat cgaaggcgtg
      421 caaggcaccc tcgccgccca acagttcgtt gaaaaggtcc tcgaggaaaa accgttcgtc
      481 cgcaacccgg ccgccgcgga gcaggaggcg ctcacctcct ccctcgccgc actggagaac
      541 gccgatgccg ccacgcgggc gcggggcgtc gcggaactga cccggttcct cacgggaccg
      601 aatcccgacc tctccacgct gaaaagcctg gtggcggtcc tcgaaaacga ttccccggcc
      661 gtggccgagg tgcgccaaat actgcagacc gcgggagagc ccgccatccc gcttctgacg
      721 gcgctgatgg gcaacgccca aaaatcggcc gccgcccggg cgcgggccgc tgaaacgatc
      781 acccccatcc tccgcacgct tcgcggccga cagtcttacg cgcggtttca aaaaactctc
      841 tcgaccaaca aagtgctcga agctctgtcc gtctcggaat cggaaaaacg atcggagatg
      901 gcggccgcgc tggcggagtt gcgcaccgtg cttcctggaa aggccgaacg cgcccggctg
      961 gtcgccgcca aaaccctttg ggtcgccggc ggggccatca ccctcgtcgc cgtcatcgcc
     1021 ggcaaggcct cgcctctctc tctggcggcc ctggggatcg ctttcgccgc gcaacgtctc
     1081 ttcccgaaga acacgctgtt gcagcgggtt ctcccgctcg ccttcgtgct cgtcagcgcg
     1141 gcggccctgg gcggcctgtt gcatgtcctg ccgttcctgg ccctctctct catcacctat
     1201 caggccatgc gctggatgcg ccgggcgccg cgatgggccc gcctcgcgtt ccttgtgccg
     1261 gccctgttcc tcgtctcctc cggcagcgcg gcggccgcgg aactggtcaa agacggacag
     1321 gcgtggctcc tgggcggcca gaaattcgac tggatttcct tccgggccca agccgacctg
     1381 aaacaggccg gtctcatcaa caccgacacc tccgccgccg acgcccaacg attgatcgcg
     1441 gaacgcgtgg acgaatacac cgaattgatg cgggccaaag tcgatccgaa ttggaacccc
     1501 aacaacgtcc acgaaggaga catcgtcctt cggccgccgc tgggcgaaac cgtccccgaa
     1561 gccgaagtga ccgtcacccc tgtgtcggaa acgcccgccg tcgttcggga atcgtcccct
     1621 caaaccgaat caaccgccgt ccgggaggaa gcctccctgg cccctcaagc cgacagcgct
     1681 ctctccgccg tttcggacgt cctgctcccc gcccttctcg ttctggcggc cgtgtccgtc
     1741 ctggtctttt tcctttatcg ggcctaccgc cgccggctca accaattggc ggatcccatt
     1801 tcgttcagcc gttacctttt ccgcgacctg ccgggccgtc tgtgggccaa ggtccgtccc
     1861 gcggaacaag cggcggaaac cgtcgaggag gcgaaaacag ccgagacggc atcctctctc
     1921 tctctccaac cgacgccgcc ggcccccacc gacgtcgagg ccccggtgga aaaagaagcg
     1981 cccgccccgg gcacacagat ggaactcttc cgtccggagg aattgcctcc ggcgactccc
     2041 gtcgcggcgc cgaaacccgc cgacgtcaaa accgcggcgg ccgacaagag caaaaaaggc
     2101 gcggaggcgg ttcaactttt gttggatttc gaacaacaac agcaacaaca aacggcggcg
     2161 ggacccaagg ccgaagaaaa aaccctcacc gccgtcaaca tcaacgagct ctccatcgaa
     2221 caactggcgg cgctcctcaa agaagccggc gtccggctgc cttacagccg cgtcgcaacc
     2281 aacatcttca atcatgtccg ctccaaagga ccccttctct ccgcggaagc gctgatcgac
     2341 gcgcatctgg ggatcggcca gaaatcctgg gaacgcctgg cgaaatattt cattttgacg
     2401 gcgccggaaa agccggcgga gcccgccgcc gatcaggccg caccggcaaa atcgatcggg
     2461 ccggacacga ccgtcccgtc ccaggacgaa ccgacgccgg cctccctggt ggacaatctg
     2521 gcggaaacgg cccaggacgt ggcgcgcgac gtcctgccgg acgttttgga gcgccccttg
     2581 cggacggtga ccgacgccgt ccttccgtcg gcggacgaga catcggccgc ggaggagccc
     2641 gcggcggagg aaccgacccc gccacaaaaa atggaccaag ccgctgagcc tctcgacatc
     2701 aacaaggcga gcgtggaaca gctggccgcc ctcctgaaga aaacgggcgc ggtgatcgcc
     2761 tacaataaag tggccgaacg gattgccgcc cgcatcaagg aaaaaggccc gctcggctcc
     2821 gctcaggaat tgctcgacgc ccacgtgggg gtcggccaaa aagcctggga aaaactggcg
     2881 cagtccctca cctttggcaa agaaggaacc ggcggagaag ccgccgacca ggagccggcc
     2941 gacacggatc gggcggaaga accgtctccc atcgagggga tggcccgggc ggcgaaagaa
     3001 accgtggacg gcttcatgcc gaccctgccg caaacagcgc ccgccgaggc gacggccgag
     3061 gaaaaaccgt cgtcggaaca gtccgccacg aaaacgacaa aaacgcgcaa accaaaaacg
     3121 accaagaaag acgccccggc ggcggtctcg gaatcctccc cgatcgcggc ggaaatgccg
     3181 gtgg
//
LOCUS       DAOTFD010000272         3151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_23831_length_3151_cov_3.994186, whole genome shotgun sequence.
ACCESSION   DAOTFD010000272 DAOTFD010000000
VERSION     DAOTFD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23831_length_3151_cov_3.994186"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3151)
                     /locus_tag="P5079_11065"
     CDS             complement(<1..>3151)
                     /locus_tag="P5079_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30559.1"
                     /translation="VLFLLLPAKKRRVVAEFMSNFYLFATYGWIAVLGGSTAASGPLF
                     LLGFYVVFKIFFLLARRYQEGVFLFVSNRVSSASTKVLSTKLGGKFLQPWKTRMDTAR
                     ALIALEESEEGTPRPSLWKLAFPTMVAGFATFFLRIGYAFGKAHWEKHTGTSDLEKDV
                     FLGLHESNTFPKLSAAAEAGLRRGFVTYNVWLNAAGQPTLTDEEIREALAPLAGRPDN
                     FDDISKAIMEVERRMDVITARVFGRLNLSETDPDALQAPFTPKMFQGLTAEFLKPMPN
                     NNTDFIVQARSAVEMLLSRQSQLIKDMSKNVLDNIGREAQTLPRFNDAAMHRYLSLAV
                     LSYQHNLRPQLQFVSQGDPELQRLAGAVDQAQNGLLTAKTPPDILLRLKDLSSRLDDF
                     FKTLERKQLLDPRGAFRAPSIRFNAGNIRSLIETNLPSDTADPENLRPDLLFRTYQTI
                     ESLSEKSLAEYVKHGIAGGFEPETMFVDYKGALLRETPAQISTAFEQRILPVLHLLSQ
                     DNTFQARMKAIETDLAALKQLSARGGRGELTPAESKLMADRVQHLATSTEQLLADAFE
                     KREKLIMGDKMKGLVPGHPYEELLKSSTFFRQGHLSARVRELLTPEHGSREGIRSGYL
                     LNAYRELEVVTQQAMNSYIRVQITDVGELTSKILEYKIRERNWTDYITTLGFTTGNYF
                     LGKLGLTVDQGWVESLFQEKGDVGPFVLRMRVIHQHLLTPNIYLLSGGDQALTQEFAK
                     MFDDIFRGKDVVNKPGELQKALEEYFTRMDRINAALPGVQRHGEAIQEITLMNKRLAM
                     DRNSQLVGLSRDYANAGAQYYFNQRQDGLRQQMIDQRAERASSANDYARQLHDMNEQI
                     NSQKRENRIMDLYHRGGTASEGTALYEQYQLLKNLQNQGLSSFEIYRRIWPDDSLSQP
                     TAFTPTAYSDSTTLPNRTPLGVRQEFVTPLWRSFLYGVSFFKDPKRVGTMQSAYLSAI
                     QHMQSEFSQTIENRSIRDPLGLGLSEPQLMAAAVSSPKDVLPVINGLRTQHANILEEA
                     RRVTADPAGFKKYEDAVARL"
BASE COUNT          527 a          861 c         1095 g          668 t
ORIGIN      
        1 aaggcgggcg acggcgtcct cgtatttctt gaaacccgcc gggtcggcgg tgacccggcg
       61 cgcttcttcg aggatgttgg cgtgctgcgt gcggagaccg ttgatgaccg ggaggacgtc
      121 cttggggctg gaaacggcgg cggccatcag ttgcggctcg ctgaggccca gccccagagg
      181 gtcacggatg ctgcggttct cgatggtctg cgagaactcg gactgcatgt gctggatggc
      241 ggacaaatag gccgactgca tggtgccgac gcgtttggga tccttgaaga agctgacgcc
      301 gtaaaggaag ctccgccaga ggggcgtgac gaactcctgg cgcacaccca ggggggtgcg
      361 gttaggcagg gtcgtgctgt ccgaatacgc ggtcggcgtg aacgccgtcg gctggctcaa
      421 agagtcgtcg ggccagatcc gccggtaaat ttcgaacgag gaaagcccct gattctggag
      481 gtttttaagg agttggtatt gttcgtacag cgcggtcccc tcggacgcgg tccccccgcg
      541 atggtacagg tccatgatgc ggttttcccg tttctgggag ttgatttgct cgttcatgtc
      601 gtggagttgg cgggcgtagt cgttggcgga cgacgctctc tccgcgcgtt ggtcgatcat
      661 ttgttggcgc agaccgtcct ggcgttggtt gaaatagtat tgcgcgcccg cgttggcgta
      721 gtcccggctg agccccacca gttggctgtt ccggtccatg gccagccgct tgttcatcag
      781 cgtgatctcc tggatcgctt ctccgtgccg ttgcacgccc ggcagggcgg cgttgatgcg
      841 gtccatgcgg gtgaaatatt cttccaaggc cttctgcagt tcgcccggct tgttgaccac
      901 gtctttcccc cggaaaatgt cgtcgaacat cttggcgaat tcctgggtga gcgcctggtc
      961 gccgccggag agcagataga tgttcggcgt gagcaggtgc tgatggatga cccgcatgcg
     1021 gaggacgaac ggccccacgt cgcctttctc ttggaacagc gattccaccc agccctggtc
     1081 cacggtcaag ccgagtttgc cgaggaaata gttgcccgtg gtgaagccca gggtggtgat
     1141 gtaatccgtc cagttccgtt cccggatctt gtattccaat atcttcgagg tgagttcgcc
     1201 gacgtccgtg atctgcacgc ggatgtagga gttcatggcc tgctgggtca cgacctccaa
     1261 ctcgcggtag gcgttcaaga gatatccgga acggattccc tcgcggctgc cgtgttccgg
     1321 cgtcagcaac tccctcaccc gggccgacag gtgaccctga cggaagaagg tggaggactt
     1381 gaggagttcc tcataggggt ggccggggac aaggcccttc atcttgtccc ccatgatgag
     1441 cttttcgcgt ttttcgaaag cgtccgcgag gagttgttcc gtggaggtgg ccaggtgttg
     1501 gacgcggtcg gccatgagct tgctctccgc cggggtcagt tccccgcggc ctccccgagc
     1561 ggaaagctgc ttgagggcgg ccaggtcggt ttcgatggcc ttcattcggg cctggaaggt
     1621 gttgtcctgg gacagcaggt gcagcaccgg caggatgcgt tgttcgaagg cggtgctgat
     1681 ctgggccggg gtttcccgca ggagggcccc cttgtaatcg acgaacatcg tttccggttc
     1741 aaagccgccg gcgattccgt gtttcacgta ttcggccagg gatttttccg agagggactc
     1801 gatggtctga taggtccgga agagaaggtc cggccggagg ttctcggggt cggcggtgtc
     1861 ggacgggaga ttggtttcga tgagcgatcg gatgttcccg gcgttgaacc ggatggacgg
     1921 cgcgcggaag gccccgcgcg ggtccaacaa ctgtttgcgt tccagcgtct tgaagaaatc
     1981 gtccaggcgg gaggagaggt ccttgagccg caggaggatg tcgggcggcg tcttcgccgt
     2041 gaggagaccg ttttgggcct gatccaccgc tccggccagt ctctgcagtt cggggtcgcc
     2101 ctggctgacg aactgcagct gcggccgcag gttgtgctga taggagagga cggccaggga
     2161 gaggtaacgg tgcatggccg cgtcgttgaa acgcggaagg gtttgggctt cccgtccgat
     2221 gttgtccagc acatttttgg acatgtcttt gatgagctgg ctttggcgcg aaagcagcat
     2281 ttccacggcg ctgcgcgcct gcacgatgaa atccgtgttg ttgttgggca tgggcttgag
     2341 gaattcggcg gtgaggccct ggaacatttt gggcgtgaag ggggcctgca gggcgtcggg
     2401 gtccgtttcc gaaagattga gacgtccgaa gacccgcgcc gtaatgacgt ccattcgccg
     2461 ttccacttcc atgatggctt tggagatgtc gtcgaaatta tccggccggc cggcgagagg
     2521 cgccagcgct tcgcggatct cctcgtcggt gagggtcggc tgtccggcgg cgttgagcca
     2581 gacgttgtag gtgacgaacc cgcgtcgcag cccggcctcg gccgcggcgg agagtttcgg
     2641 gaaggtgttg gattcgtgca ggcccaggaa gacgtctttt tcaaggtccg aggtgccggt
     2701 gtgcttttcc cagtgggctt tgccgaacgc gtagccgatg cgcaggaaga acgtggcgaa
     2761 cccggccacc atggttggga aggccagttt ccacaggctc ggccgcggcg ttccctcctc
     2821 cgactcctcc agcgcgatca gggcccgcgc cgtgtccatg cgggttttcc agggctggag
     2881 gaatttccct ccgagcttgg tggagagcac tttggtgctg gcggaggaga cccggttgct
     2941 gacaaaaagg aagacccctt cctgatagcg ccgcgccagg aggaagaaga ttttgaagac
     3001 gacgtagaat cccagcagga acagcggacc gctggcggcg gtggagccgc ccagcacggc
     3061 gatccagccg taggtggcga agaggtagaa attcgacatg aactccgcca cgacgcggcg
     3121 tttcttggcg ggcaacagga ggaacaacac c
//
LOCUS       DAOTFD010000273         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_23971_length_3139_cov_4.177691, whole genome shotgun sequence.
ACCESSION   DAOTFD010000273 DAOTFD010000000
VERSION     DAOTFD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23971_length_3139_cov_4.177691"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="P5079_11070"
     CDS             <1..453
                     /locus_tag="P5079_11070"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRY30560.1"
                     /translation="LPAGPVRVRDVYYVAPFANTVVTMRLRGEQIWRLLEHGVSGRQG
                     FLQVSGVRLKYDPAQTVGQRVRAVWVGESPLDRGRLYRVATNSFLAQGGDGYSAFAEG
                     IDRQDTGMKLLDLLVREFENKKQVSAPRGGRIIVMRGSDVPVPEKTAY"
     gene            509..2404
                     /locus_tag="P5079_11075"
     CDS             509..2404
                     /locus_tag="P5079_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY30561.1"
                     /translation="MIASFEKGTAFLRRLRLPAFLARGIPPKGLRYRLLGFFVLSVLV
                     PTLLTGTVVMVTIRRSLLEIPLREQREIALRVSDLVAGHVGNVKKVLLTVASRGGFAG
                     ASAAEQRAALRDLMTQYPHLTECAVLNPQGRETVKMVRQDRRLLAADALLNRAGRDEF
                     RRAWTGPGYVSPVFFTERDRTPQMFIAVPIGRRQGVLLSRLSLDSVWELVSEVSVGKG
                     GYAYLVDTKGNLLAHPQRDRVLSHENMAERSVVKELLEGESEPLGGGTGLFHLHEGDE
                     GEKVLAVHHRVTGLGWGVVVQRPMSDALAPMRSLWRRVAAVVLVLGGVLLAAGLSLVR
                     KILEPLAVLQEGVQKIAQGDLKQRLEIHTDDEIQKLAEEFNKMAESLESAEELKRDLT
                     HMIVHDLKSPLSGVLGSIDYVASGAIGQVTEEQKKILSLGVKSGKDLLRLIQNLLDMA
                     KMEEGRLELRREEFSLLELAAECVDDLEAHIHRENKQVSVEVPKSLPKIWADRDLVYR
                     VLTNLLTNALKHTSQGAEIAIGAGLSDDQSAFVLSVKDSGEGIPPEFIAKIFEKFGQA
                     EAKRKKFRIGSGLGLTFCKLAVEAHGGRIWVESELGKGSAFFVRIPRVEKPSSAPGQS
                     RETPLAV"
     gene            2442..2822
                     /locus_tag="P5079_11080"
     CDS             2442..2822
                     /locus_tag="P5079_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30562.1"
                     /translation="MDKKKILVTDDEQNIVELLSVVLRAEGYEVATASNGLEALKKIQ
                     SVKPDLVILDVNMPQLDGWNVLSTIRATESTRHLPVLMCTNKDLVSDVERAEALGATG
                     YIPKPFEIERVLMKLRNVFGQKPA"
     gene            2847..>3139
                     /locus_tag="P5079_11085"
     CDS             2847..>3139
                     /locus_tag="P5079_11085"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRY30563.1"
                     /translation="MMKDIRRIAFLVCVFAVGAAPAAWARSRNVIVMIGDGMGFSLIG
                     LSRNYSEFVLRIPPPLALQEALRLGRMGLVSNRSSSHLVTDSAAAATAMACGVR"
BASE COUNT          565 a          916 c         1088 g          570 t
ORIGIN      
        1 ctgcccgcgg gaccggtgcg ggtgcgcgac gtgtattatg tggcgccgtt cgccaacacc
       61 gtggtgacca tgcggttgcg gggagagcag atttggcggc tgttggaaca cggcgtttcg
      121 gggaggcagg gctttcttca ggtgtcgggg gtgcgtctga aatacgatcc cgcccagacc
      181 gtcggccagc gggtgcgcgc ggtgtgggtg ggggaatcgc cgctggaccg cggccggctc
      241 tatcgcgtgg ccacgaattc ttttctggcg cagggaggcg acggttattc ggcgttcgcc
      301 gaggggatcg accggcagga cacgggcatg aaactgttgg atctgctcgt ccgggagttc
      361 gagaacaaaa agcaggtctc cgcgccgcgc ggcgggcgga tcatcgtcat gcgcggttcg
      421 gatgtccccg tccccgaaaa gacggcctac tagccccgcg atttttataa gatgagaccg
      481 gtatgaagga agggaaacag ggagagcgat gatcgcgtcg tttgaaaaag ggacagcgtt
      541 ccttcggcgc ctgcgcttgc ccgcgttcct tgcgcgcggg atcccgccca aagggttgcg
      601 ttaccgcctt ttgggatttt ttgttttgag cgtcctggtc ccgaccctct tgacggggac
      661 ggtggtgatg gtgacgatcc gccggtccct gctggagatc ccgttgaggg aacagaggga
      721 gattgcgttg cgcgtgagcg acctcgtggc gggacacgtc ggcaacgtga agaaggtgct
      781 tctcaccgtg gcttcacgcg gcgggttcgc cggggcgtcg gcggccgaac agcgcgccgc
      841 gctgcgcgac ttgatgacgc aataccccca cctcaccgaa tgcgccgtgc ttaatccgca
      901 gggccgcgag accgtgaaga tggtgcggca ggaccgccgg ctcctggcgg ccgacgccct
      961 tctcaaccgc gccgggcggg acgagttccg ccgcgcctgg accggccccg gatacgtcag
     1021 ccccgtgttt ttcaccgaac gggaccggac gccgcagatg ttcatcgcgg tgcccatcgg
     1081 ccgccggcag ggcgtcctcc tctcccggct ttccctcgac agcgtgtggg aacttgtctc
     1141 cgaggtctcc gtcggtaaag gcggctacgc ctatctggtg gacaccaagg ggaatctcct
     1201 ggcccatccc cagagggacc gggtgttgtc gcacgagaac atggcggaac ggtccgtggt
     1261 gaaggaactg ctggaggggg agtccgaacc cctgggcggc gggacggggc tgttccatct
     1321 tcatgagggt gacgaggggg aaaaagtcct agccgtgcat caccgggtga ccgggttggg
     1381 gtggggcgtg gtggtgcagc ggccgatgag cgacgcgttg gcgccgatgc ggtccctgtg
     1441 gcgtcgtgtg gcggccgtcg tgctggtgtt gggcggggtg ctcctggccg cgggattgtc
     1501 tttggtgagg aagatcctcg aaccgttggc ggtccttcag gaaggcgtgc agaagatcgc
     1561 ccagggggac ctgaaacagc gtctggagat ccacaccgac gacgagatcc aaaaactggc
     1621 ggaggaattt aataaaatgg ccgagtccct ggagtcggcg gaggagctca agcgggacct
     1681 gacgcacatg atcgtgcacg atctcaagag ccccctgtcg ggcgttttgg ggagcatcga
     1741 ctacgtcgcc tccggcgcca tcggccaggt gaccgaggaa cagaaaaaga tactgtctct
     1801 gggcgtcaag tccggcaaag acctcctgcg gctcatccag aacctgttgg acatggccaa
     1861 gatggaggag ggccggttgg aactgcggcg ggaggagttt tccctgttgg agttggccgc
     1921 cgagtgcgtg gacgacctgg aggcgcacat ccaccgcgag aacaaacagg tcagcgtgga
     1981 ggtgccgaag agccttccga agatctgggc cgaccgggac ttggtctacc gcgtgttgac
     2041 gaacctgctg accaacgcgc tcaaacacac ctcgcagggg gcggagatcg ccatcggcgc
     2101 cgggttgagc gacgaccaat ccgctttcgt gctcagcgtc aaggacagcg gcgagggcat
     2161 cccgccggag ttcatcgcga agattttcga gaagttcggc caggccgagg ccaaacgaaa
     2221 aaaattcagg atcggttccg gtttgggcct tacgttttgt aaactagcgg tggaggccca
     2281 cggcggccgg atttgggtgg agtccgaact gggcaaaggc agcgcgtttt tcgtgcggat
     2341 cccgcgggtg gagaaaccgt cctccgcgcc ggggcagagc agggagacgc ccctcgcggt
     2401 gtgaacgggg cgtatcgtca accaaggagg cgagaaggcc gatggacaaa aaaaagattt
     2461 tggtgaccga cgacgaacag aacatcgtcg aacttttgag cgtcgttctg cgcgccgagg
     2521 gatatgaggt cgccacggcc tccaacggcc tggaggccct caagaagata cagagcgtga
     2581 aacccgacct cgtcattttg gacgtcaaca tgccccagtt ggacggttgg aacgtccttt
     2641 ccaccatccg ggccacggaa tccacccggc atctgccggt gttgatgtgc accaacaagg
     2701 acctggtcag cgatgtggag cgcgccgagg cgctcggcgc caccgggtac atccccaaac
     2761 cgttcgagat cgaacgggtc ctgatgaagc tccgcaacgt cttcgggcaa aagcccgctt
     2821 aagtctcctc aacgccgagg cgtcccatga tgaaagatat ccgtcgcatc gcgttcctcg
     2881 tctgcgtttt tgccgtcgga gcggctccgg cggcctgggc ccgttcccgc aacgtcatcg
     2941 tgatgatcgg cgacgggatg gggttctccc tcatcgggct gtcccgcaac tacagcgaat
     3001 tcgtccttcg aatccctccg ccgctggccc tgcaggaggc gctgcgcctc ggccggatgg
     3061 ggctggtctc caaccgctcc tcctcccatc tggtgaccga ctcggccgcg gcggccacgg
     3121 ccatggcgtg cggggtgcg
//
LOCUS       DAOTFD010000274         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_24371_length_3108_cov_2.770062, whole genome shotgun sequence.
ACCESSION   DAOTFD010000274 DAOTFD010000000
VERSION     DAOTFD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24371_length_3108_cov_2.770062"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5079_11090"
     CDS             <1..363
                     /locus_tag="P5079_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30564.1"
                     /translation="RYGGFWGVFNALFWRAYFRAAARHHFQGPRRWTCTAGRGSLYID
                     GLANVYACEMREPMGNLKQDGLGDILSSSGARSQRDAIARKECSCDHACFLQHSFLTE
                     PRNLPHLLAGMRRAFLPS"
     gene            360..1334
                     /locus_tag="P5079_11095"
     CDS             360..1334
                     /locus_tag="P5079_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30565.1"
                     /translation="MTPAAIVVVFKKWTGYVAECVRAIENLRGGPYPLVLVPDGPVPE
                     YVGSARVVVHAGSIAQKRNAGVGACPPEVPWVAFVDSDAWPRTDWMLGFQTRLDKLSS
                     SLPVVCVTGPDLTPPLDPWTRRVTGYALASPLIMGRGSLRFRVSEKAVFVRNASTCNM
                     FVRRDALSSVGGFDERLLTSEDVQLCQDLLSRGGAILFDPEIAVFHHRRDIPRFFLQW
                     FNEGLALRHYRRFPAAGNFLQHLPSVLMLSEAALLFLGWTGLGLVMCLELGQYVVFYF
                     DSLRRGTGAFLSHGVAFSMWLFVKAYGAGGMISYLFRPRKYVHSRCDH"
     gene            1475..>2306
                     /locus_tag="P5079_11100"
     CDS             1475..>2306
                     /locus_tag="P5079_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY30566.1"
                     /translation="MKEPPLLSRLKRFSKTPLLVVGDLMVDHYIRGRVDRLSPEAPVP
                     VVEITTEEFMPGGAGNVAANIAALGGVPLLVSVVGEDNPGEMLLSTLRGQGVDVDGVG
                     KDGSRPTIIKTRVIAGHQQVVRFDRENRAPLGREVIDLLVAQVERHIRNVQGIVLSDY
                     GKGVINGRLLARVLSLARRYQKSVTVDPKVEHFLRYRGVTCITPNLKEAAEGVRALPP
                     KTDREVDALGKRILKRLRCQSVLITRGERGMSLYQSSGNIVHIPSQAHEVYDVTGAGD
                     T"
     assembly_gap    2307..2316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2474..>3108
                     /gene="kdsB"
                     /locus_tag="P5079_11105"
     CDS             2474..>3108
                     /gene="kdsB"
                     /locus_tag="P5079_11105"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRY30567.1"
                     /translation="MRVLGVIPARFASTRFPGKPLFPLAGRPMIQWVYEAARRALPRV
                     VVATDDRRILSFIQGLGGEAMMTSSRCRSGTDRVAEVSRRRKADIYLNIQGDEPLMSS
                     RTVRAVLALHSRRGVCLGTAATALPDRAAWTNPDAVKVLVDNSGNALYFSRAPIPYYR
                     DGVGAASGGPFRRRDFVLKHLGVYSYAAATLARFVRWPAGFFEKAEKLEQLR"
BASE COUNT          467 a          966 c         1011 g          654 t
ORIGIN      
        1 cgctatgggg gattttgggg cgttttcaat gcgttgtttt ggagggcata ttttcgggcg
       61 gcggcacgtc accattttca aggcccacgt cgctggacgt gcaccgccgg gcggggcagc
      121 ctctatatcg atgggttggc caacgtttac gcctgtgaga tgcgcgagcc gatggggaat
      181 ttgaagcagg acgggctcgg cgacatttta tcgtccagcg gagcgcgttc tcagcgcgac
      241 gcgatcgcgc ggaaagagtg ttcctgcgat catgcctgtt ttttgcagca ctcatttttg
      301 accgagccgc gcaatctgcc gcatttgctg gcggggatgc ggcgtgcctt tttgccgtca
      361 tgacgccggc cgccatcgtc gtcgttttta agaaatggac gggctacgtg gcggagtgcg
      421 tgcgggccat cgagaatttg cgaggggggc cctatcccct ggtcctggtc cccgacggac
      481 ccgtgcccga gtatgttggc tcggcccgcg tggtggtgca tgccgggagc atcgcgcaga
      541 aacgcaacgc cggggtgggc gcctgcccgc cggaggttcc ctgggtggcg tttgtggact
      601 ccgacgcctg gccccgcacc gactggatgt tgggttttca gacccgcctt gacaaactgt
      661 cgtcgtcttt gccggtggtg tgcgtgacgg ggcccgattt gaccccgccc ctggatcctt
      721 ggacccggcg cgtgaccggc tacgccctcg ccagcccgtt gattatggga cggggaagcc
      781 tgcgtttccg tgtcagtgaa aaggcggtgt tcgtccgcaa cgcttccacg tgcaacatgt
      841 ttgtccggag ggatgcgctt tcgtcggtcg gcggttttga tgagcgcctg ttgaccagcg
      901 aagacgtcca gttgtgccag gatctccttt cccggggggg cgccatcctc ttcgaccctg
      961 aaatcgccgt ttttcatcat cggcgcgaca tcccccggtt tttcctgcaa tggttcaacg
     1021 aggggttggc cctgcggcat tatcgtcgtt tccccgcggc gggtaatttt ttgcaacacc
     1081 tgccgagcgt gttgatgctg tcggaggccg ctcttctctt tttgggctgg acgggactcg
     1141 gcctggtgat gtgtctcgag ttgggacaat acgtcgtttt ttatttcgat tccctccggc
     1201 gcgggacggg ggccttcttg tcgcacggcg tcgccttttc catgtggctg ttcgtcaagg
     1261 cgtacggggc gggggggatg atcagttatc tgttccggcc gcggaaatac gtccacagcc
     1321 gatgcgacca ctaggcgtcg cccacggttg atgttttgtc ggcaatttgc taaaaaattg
     1381 cgcatgaaat cgcccaatcc gagggatatc ccgggtttta ttcgggatcc gcaatggact
     1441 ttgtcctaag tccatcctgc cgcgaatgat tcctatgaaa gaaccgcccc tcctttcccg
     1501 cttgaaacgt ttttccaaga cgccgctcct cgtggtcggc gacttgatgg tggaccacta
     1561 tatccgcgga cgcgtggacc gtctttcccc ggaggccccc gtcccggtgg tggagatcac
     1621 caccgaggaa ttcatgcccg gcggcgccgg caacgtggcc gccaacatcg ccgccttggg
     1681 cggcgttccg ttgctggttt ccgtggtggg ggaagacaac ccgggcgaga tgctcctgtc
     1741 caccctgcgg ggccaggggg tcgatgtgga cggcgtgggg aaggatggat cccgtcccac
     1801 catcatcaaa acgcgggtca tcgccggtca tcagcaggtg gtgcggttcg accgtgaaaa
     1861 ccgcgccccc ctcggccggg aagtgatcga tctcttggtg gcgcaggtgg agcgccacat
     1921 ccggaacgtc caggggatcg tgctgtcgga ttacggcaaa ggggtcatca acggccggct
     1981 cttggcgcgc gtcctctcgc tggctcgccg ctaccaaaaa tcggtgacgg tggatcccaa
     2041 ggtggaacat tttctccgtt accgcggcgt gacctgcatc acgcccaacc tgaaggaggc
     2101 cgcggagggc gtccgggcgc tgcctcccaa gaccgaccgg gaagtggatg ccctcggcaa
     2161 aaggattttg aaacgtctcc gttgtcagtc ggtgttgatc acccgcggtg aacgggggat
     2221 gagcctgtac cagtcgtcgg gaaacatcgt gcacatcccg tcccaggccc acgaggtgta
     2281 tgacgtcacc ggcgccggcg acacggnnnn nnnnnnccac gttcagcctg gcgttggcgg
     2341 cggggagccc gctcgacgag gccgcgcgcc tgtccaacct ggcggccggc gtcgtggtgg
     2401 ccaagctcgg caccgccacg gtgcgcccga gagagatacg agccgccctg tcgcgttgga
     2461 acggccgagg gtgatgagag tcctcggagt catccccgcc cgattcgctt ccactcgttt
     2521 tcccggtaaa cccctctttc ccctggccgg ccggccgatg atccagtggg tatacgaagc
     2581 cgcccgccgc gcccttccgc gcgtcgtggt ggccacggac gaccgccgca tcctttcgtt
     2641 catccagggg ctgggcggag aggcgatgat gacctcctcc cgctgccgga gcggcaccga
     2701 ccgcgtggcc gaggtgtcgc gccgccggaa ggcggacatc tatctcaata tccaggggga
     2761 cgagccgctc atgtcttccc gcacggtccg cgccgtcctg gcgctgcatt cccgccgggg
     2821 cgtgtgcctg ggcacggcgg ccacggccct gccggaccgc gcggcctgga ccaaccccga
     2881 cgcggtcaag gtgctcgtgg ataattccgg caacgcgctc tatttttccc gcgcccccat
     2941 cccttattac cgggacggcg tgggggccgc ctccggcggc ccgttccgcc gccgcgattt
     3001 cgttttgaaa catctgggcg tctactccta cgccgcggcg accctggccc gtttcgtccg
     3061 gtggccggcc ggttttttcg agaaggcgga gaaactcgaa caacttcg
//
LOCUS       DAOTFD010000275         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_24634_length_3086_cov_4.967008, whole genome shotgun sequence.
ACCESSION   DAOTFD010000275 DAOTFD010000000
VERSION     DAOTFD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24634_length_3086_cov_4.967008"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="P5079_11110"
     CDS             complement(<1..1541)
                     /locus_tag="P5079_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY30568.1"
                     /translation="MARKFEQLHPGVAVETQAIPWEAAHAKLLTSVVGGIPPDVSQLG
                     TTWMAEFAAMRALEPLDAHAQRSSVAKPAAFFDGSLRTCRVDGILYGLPWYVDTRVLF
                     YRKDLLAEVGFKEPPKTWEELKVAGRRLAERKNPDGKKSFGVALGTRSWTELLLFVWQ
                     NGGDPLRPSAMEFFDAMRFYISLFREGITPTKEAADLNKLHAFKTGFFPMFVSGPWEV
                     ELLRKELPELEGRWGIGLLPGKKSRTSFVGGCNLVVFRDSRKKELAWEFLEFMSEPQN
                     QIEWLQLTNDLPSVKAAWEHAYFADKPLLKVFGRQMYDTASPPTIPEWEQIASLTENA
                     MEKIILDEHITDNGVMEQLLTLEASILKLRSENSARPGTWRAFLLFIAALVALGGGFW
                     MLRSFPRLKITGDKTKAPALSWQEFRRKDLFGLLFILPALLLLVVFLFLPIAVSFVMS
                     LTDLNIFSINQWSRIQFVGWQNYLRILQDPLFWKSLWNTLYFAGVGVPLTIAVSLLAA
                     VALNQ"
     gene            complement(1787..>3086)
                     /locus_tag="P5079_11115"
     CDS             complement(1787..>3086)
                     /locus_tag="P5079_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30569.1"
                     /translation="ITNAKLGDYSARAEIGSASQEFGQASAYVGSSTTLNTNRFANVE
                     MPSFQVMANNVSPYLTNMTVGNLFVDYSPYVFSPVFGYKGLSVEGDYDRFNYHAFALK
                     HVLNSFTAGTRARGIWPNFKLTGYGVYWEQNARLANPAAVSGNTLTTAPSSVIELRRV
                     AQDLVYNLDGEYRLLADRVTLQGIYGINRYSQSASVDYTDPFNPTFGLPLDKTLSPTG
                     EMARGKIESNGLLWKGLNTAYSYRDMGTEYKPHYRQNPMSFDDTESDQWGHNVRLTQF
                     HKGWVFSNEYDTLRRHSMRRYFRHRYTWGVGHYGYKGMDIAFSQEIRREIYAFASDRS
                     NFTTDKNEQVVGNELYVRTQLSPRMAFWVKPRQEHVRHPATNRRFVTDSLQTKLEFYI
                     TTNARLFAEHKVTRFGDKTFEPQGYPFDDNFTRVSFEVTF"
BASE COUNT          565 a          828 c         1080 g          613 t
ORIGIN      
        1 ttctggttga gcgccaccgc cgccagcaac gacaccgcga tggtgagcgg cacccccacc
       61 cccgcaaaat aaagggtgtt ccagagcgat ttccagaaga gcgggtcctg caggatgcgg
      121 aggtagtttt gccagcccac gaactgaatg cggctccatt ggttgatgct gaagatgttg
      181 aggtccgtga ggctcatcac gaaggagacg gcgatgggaa ggaaaaggaa gacgaccagg
      241 aggagcagcg ccgggaggat gaacaggagg ccgaagaggt ccttccgacg gaactcctgc
      301 caggaaaggg ccggggcttt ggttttgtcg cccgtgattt tcagccgggg gaacgacctc
      361 agcatccaga acccgccgcc caacgcgacg agcgccgcga tgaacaggag gaaggccctc
      421 caggtgcccg gccgggccga attttcggaa cgcagtttga ggatggacgc ctccagggtg
      481 aggagctgtt ccatgacgcc gttgtcggtg atgtgctcgt cgaggataat tttttccatg
      541 gcgttttcgg tgagcgaggc gatctgttcc cattccggga tggtgggcgg gctggcggtg
      601 tcgtacatct gccggccgaa gaccttcagc aggggtttgt cggcgaaata ggcgtgctcc
      661 caggccgctt tcaccgacgg caaatcgttg gtgagctgga gccattcgat ctggttctgc
      721 ggttcgctca tgaactccag gaattcccac gccagttctt ttttgcgcga atcgcggaag
      781 acgacgaggt tgcacccgcc gacgaacgag gtgcgggatt ttttgccggg gagaaggccg
      841 atgccccatc ggccttccag ttccgggagt tccttgcgca gcagttcgac ctcccacggt
      901 ccggagacga acatggggaa gaagccggtc ttgaaggcgt ggagtttgtt gaggtcggcg
      961 gcctccttgg tgggggtgat gccttcgcgg aagaggctga tgtaaaaccg catggcgtcg
     1021 aagaattcca tggccgacgg gcggagcggg tcgccgccgt tctgccacac gaagaggagg
     1081 agctccgtcc agctgcgcgt gccgagggcc acgccgaagg attttttccc gtccggattc
     1141 ttgcgttcgg ccagccgccg gccggcgacc ttgagttctt cccaggtttt gggaggttcc
     1201 ttgaacccga cctcggccag gaggtccttc cggtaaaaaa ggacgcgggt gtccacatac
     1261 cagggcaggc cgtagaggat cccgtctacc cggcaggtgc gcagggaccc gtcgaagaac
     1321 gccgccggct ttgcgacgga cgaccgctgc gcgtgtgcgt cgaggggttc cagcgcccgc
     1381 atggccgcga actcggccat ccaggtggtg cccagctggc tgacgtccgg cgggatgccc
     1441 cccaccacgg aggtgaggag tttggcgtgg gcggcttccc acgggatggc ctgcgtttcc
     1501 accgcgacgc cggggtggag ctgttcgaat ttgcgcgcca tgagggcgat cttcttgccc
     1561 tcctccccca tggcccagac gacgagtcgc cggtcggagg ccgctaaaag ttgaaagttg
     1621 aaagcgaaaa gggcgaaaga gaggagaaga ggtttaaagt taaaagttga aagttgaaag
     1681 ggggatgcga aagccaaaaa ccgtccgatc ggtgcgataa cgatgtgagg gtgtttctgt
     1741 cccccccttg aacttttaac gtttaacctt gaactgtcca cttggcctag aacgtcactt
     1801 cgaaggagac gcgggtgaaa ttgtcgtcga agggataccc ctgcggctcg aaggtcttgt
     1861 cgccgaaacg cgtgaccttg tgttcggcga acagccgggc gttggtggtg atgtagaact
     1921 ccagcttggt ctgcaggctg tccgtcacga agcgccggtt ggtggcggga tgccggacgt
     1981 gttcctggcg gggtttcacc cagaaggcca tgcggggcga gagctgggtg cgcacataga
     2041 gttcgttgcc gacgacctgc tcgttcttgt cggtggtgaa attcgaacgg tcgctggcga
     2101 aggcgtaaat ttcgcgccgg atctcctggg agaaggcgat gtccatgcct ttgtacccgt
     2161 aatgccccac gccccaggtg tagcggtggc ggaaatagcg ccgcatcgag tgccgccgca
     2221 acgtgtcgta ttcgttggag aagacccagc ccttgtggaa ctgggtgagg cggacgttgt
     2281 gtccccactg gtcgctttcg gtgtcgtcga acgacatggg gttctgtcgg taatggggtt
     2341 tgtattcggt gcccatgtcg cggtagctgt aggcggtgtt gagccctttc cagaggagcc
     2401 cgttggattc gatcttgccg cgcgccatct cgcccgtcgg agaaagcgtc ttgtcgagcg
     2461 gtaggccgaa ggtcgggttg aacgggtcgg tgtagtccac cgaggcggac tggctgtagc
     2521 ggttgatgcc gtagattccc tggagggtga cgcggtcggc caggaggcgg tattctccgt
     2581 cgaggttgta gacgagatcc tgggccaccc ggcggagttc gatgaccgac gacggcgccg
     2641 tggtgagggt gttccccgag acggcggcgg ggttggcgag gcgggcgttc tgttcccaat
     2701 acacgccgta tccggtgagt ttgaagttgg gccagatgcc gcgcgcgcgc gtgccggcgg
     2761 tgaacgagtt gagcacgtgt ttcagcgcga aagcgtggta gttgaaccgg tcgtagtccc
     2821 cctccaccga caggcccttg tagccgaaca ccggggagaa cacgtacggc gaataatcca
     2881 cgaagaggtt tcccacggtc atgttggtga ggtagggcga gacgttgttg gccatcacct
     2941 ggaagctggg catctccacg ttggcgaagc ggttggtgtt gagcgtggtg gaggagccga
     3001 cgtaggcgga ggcctggccg aactcctgcg aggcggaccc gatctccgcc cgggcgctgt
     3061 aatcgccgag cttggcgttg gtgatg
//
LOCUS       DAOTFD010000276         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_24861_length_3067_cov_3.970120, whole genome shotgun sequence.
ACCESSION   DAOTFD010000276 DAOTFD010000000
VERSION     DAOTFD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_3067_cov_3.970120"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="P5079_11120"
     CDS             <1..2053
                     /locus_tag="P5079_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30570.1"
                     /translation="PETDALHVTEGVAYLYASRGLFVRNVLRPFVMADLTHERVERVM
                     ARRQASARKGEALALFLPPFMSLDPRRFLAALASWPNLLALAVGRIVSKTAPRAPSPG
                     IVRRLASFSGRARAAALEELYQQGESLGHQALEQLSFLDPDFSSLWVSRAAPFTAVGI
                     VAGEGDFSAIQRRWGASRSASPMPAFDIASSGRRVTVVSPSVADGPVADFLRSVGAGV
                     GYAELSVGDVAEATQRLQKRSHANIRLLSAAPLTDGEDSRANFVVVKTVSGKEVLLKL
                     MEDRVSPTPARAAAALARVCERIGDRMAAGSAVSAASINEAASLLGLLHGGEGTLSEE
                     LGRHPEWVSPQALRSLRDGLRARFQDRKELARALVSVLPKIQYQLKGPKFQAQVLTVE
                     DLFGDERTDRDESRWGVVSLLSGAGAGGTVSESDQESSAGRARELEALLQTVLLLETM
                     AEKPGTSVDEAAAVYNFAHGVKRYLDEQRARPAELFVDLNRPLTQDADKKTVDGVLSL
                     DLFGADLETTDTDAAAVLLAKQIRSLRSGAEVRVPLTTLTVDVPGVSAEAVLSRFESA
                     LSRLVAEGEITPAEFNAARFSWLAVDRDLLQSAGLLKRTGQIDSDAFHRWLYRTQSAA
                     WKLSKNFVLSIVTQDQQRWTPSRKDYIRLLQIVLPEVAVRVTHDLNEYLKSQKVLEIQ
                     A"
     gene            2330..>3067
                     /locus_tag="P5079_11125"
     CDS             2330..>3067
                     /locus_tag="P5079_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30571.1"
                     /translation="MIAESREAENSVFRLLERVFLRPLPHTPSFTKNLPSWIFLRRVT
                     ALTTAVIVFYSQVVCAGTSFFPTVNLPFLKLGPRVESLMDRLNQWASDGREVFSYVMH
                     ASVYEDRQKLLNPGAAPDTLLRQEERQKANMDLQQINQQNRGMQLPSILKQPGDQRPE
                     GTLKDIKLLDEGVLPGDQVSDAIQREIEEIRSKNNKLLGEGAEFKYQPSLNFDGTLRV
                     TYFKNGNVSSIENARYRNPHGQTIIQNV"
BASE COUNT          545 a          932 c          974 g          616 t
ORIGIN      
        1 gccggaaaca gacgctctcc atgtgacgga gggcgtggcg tatctgtatg cgtcgcgcgg
       61 gctctttgtc cggaacgttc tgcggccgtt cgtgatggcc gatttgaccc acgaacgcgt
      121 cgaacgggtc atggcccgcc gtcaggcctc cgctcggaaa ggagaggctc tcgcgctgtt
      181 tctgccgccg ttcatgagcc tcgacccgcg ccggttcctc gccgcgctgg cgtcctggcc
      241 gaaccttctg gcgttggcgg tcggccggat cgtttccaaa acggcgcccc gggccccctc
      301 ccctgggatc gtccggcgac tggcctcctt ctcgggccgg gcccgcgcgg ccgcccttga
      361 ggaactttat caacagggag agtccctcgg ccaccaggcg ttggaacagt tgtccttcct
      421 tgatccggat ttttcgtctt tgtgggtgtc gcgtgcggcg cctttcacgg cggtggggat
      481 tgtggccggc gagggggatt tctccgccat acaacgccgg tggggcgcct cccggtcggc
      541 gtctccgatg ccggcgttcg acatcgcgtc ttccggacgc cgggtgacgg tcgtttcccc
      601 gtcggtggcg gacggaccgg tggcggattt cctgaggagc gtcggtgccg gggtcggtta
      661 tgcggagctt tccgtcgggg acgtcgccga ggcgacgcaa cgcttgcaga agaggtccca
      721 cgcgaacatt cgtctcctgt ccgccgcgcc gttgaccgac ggggaggatt cccgtgcgaa
      781 tttcgtggtc gtcaagaccg tttccgggaa agaggttttg ttgaaattga tggaggaccg
      841 ggtttctccg actcccgcgc gggccgcggc cgcgttggcg cgcgtctgcg agcggatcgg
      901 cgaccgaatg gccgcgggga gcgccgtttc ggcggcttcc atcaatgaag cggcgtccct
      961 gctggggttg ctgcacggcg gcgaagggac cttgtccgag gagttgggcc gtcatccgga
     1021 atgggtcagc ccacaagcgc tgcggtccct tcgggacggc ctgcgcgcga ggtttcagga
     1081 ccggaaagaa ttggcccggg cgttggtgag cgtgttgccg aagattcagt atcaattgaa
     1141 ggggccgaaa ttccaggccc aggtcctcac cgtcgaggac ctcttcggag acgaacgcac
     1201 ggaccgcgac gagtcccggt ggggggtggt ctccttgctc tccggcgccg gggcgggggg
     1261 aacggtttcc gagtcggacc aggaatcctc ggcggggcgg gcgcgcgaac tggaggcgct
     1321 cttgcagacc gttctcctgc tggaaacgat ggccgaaaag ccggggacat cggtggacga
     1381 ggcggccgcc gtctataatt tcgcgcacgg ggtgaaacgc tatctcgatg aacaacgcgc
     1441 ccggcccgcc gagcttttcg tggatttgaa tcggcccctc acgcaggacg ccgacaagaa
     1501 gacggtcgat ggcgtgttgt ctttggacct cttcggtgcc gacctggaaa cgaccgacac
     1561 tgacgccgcg gccgttctcc tggccaaaca gatccgttcg ttgcggtccg gggcggaggt
     1621 ccgtgtccct ctgacgaccc tcaccgtgga cgtccccggc gtttccgccg aggcggtgtt
     1681 gtcccgcttc gaaagcgccc tgtcccgctt ggtggccgag ggggaaatca ccccggcgga
     1741 attcaatgcg gcgcgcttct cctggctggc ggtggatcgg gacctgcttc aatccgccgg
     1801 tcttctgaaa cggacgggac agatcgattc ggacgctttc catcgctggc tctatcggac
     1861 gcagtcggcc gcgtggaaac tgagcaagaa cttcgtcctg tccattgtga cgcaggacca
     1921 acagcgatgg acgccgtcga gaaaggacta tatccggctg ttacagatcg tcctgccgga
     1981 ggtggcggtc cgcgtcaccc acgatttgaa cgagtatctg aaatcccaga aagtcctgga
     2041 aatacaggcc taatcgtttt ttcaatcgtt cagttattga acgatcgggg tttacaagcg
     2101 atatacacgt ccttcctctt cccggggagg gtttttttat gtccaggaac gttctgtcga
     2161 cggtaaaatg gggggaataa aacgcgcggg aggggtcgct gaaacaaggt tgaaaacggt
     2221 cgtttacagg ttgtttaaga gctttttacc actgctttac cgattttttc ctgcttcttt
     2281 acaagaactt aaggcgaatt ctgggtaagt cgtatatatt tataacgtca tgatcgccga
     2341 gagccgagaa gccgagaact ccgtattccg tctgctggag cgggttttcc tgcgccccct
     2401 gccccacacc ccctcgttta ccaaaaatct gccctcctgg attttcctcc gccgcgtcac
     2461 ggccctgacc acggccgtga ttgtttttta ctcccaagtg gtctgcgccg gaacctcgtt
     2521 cttcccgacg gtaaacctgc cgttcctgaa gctggggcct cgggtggaat ccctcatgga
     2581 ccgtctcaat cagtgggcca gcgacgggcg ggaggtgttc tcctacgtga tgcacgccag
     2641 cgtctacgag gaccgtcaga aactgctcaa tcccggcgcc gccccggaca ccctcctgcg
     2701 ccaggaagaa cgccaaaagg ccaacatgga ccttcagcag atcaaccagc agaaccgcgg
     2761 catgcagctc ccctccatcc tgaaacagcc gggggatcag cggccggaag ggacgctcaa
     2821 ggacatcaag ctgttggacg aaggggtgct gcccggggac caggtcagcg acgccatcca
     2881 gagggaaatc gaggagatcc gctccaaaaa caacaaattg ctcggcgagg gggcggaatt
     2941 caaataccag ccctccctga acttcgacgg gaccctgcgc gtgacctatt tcaagaacgg
     3001 gaacgtgagc tccatcgaga acgcccgcta ccgcaacccc cacggccaga ccatcatcca
     3061 gaacgtc
//
LOCUS       DAOTFD010000277         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25095_length_3047_cov_4.878008, whole genome shotgun sequence.
ACCESSION   DAOTFD010000277 DAOTFD010000000
VERSION     DAOTFD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25095_length_3047_cov_4.878008"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="P5079_11130"
     CDS             complement(<1..431)
                     /locus_tag="P5079_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30572.1"
                     /translation="MSDIESVKPQEMKWPSRRGAYYNIADSMTALPPALGAEEVPFFE
                     RLDLAYRTLCGVLYNFVPTSGHPGGSISSGRFVEGLLYRALSYDFSDPLRNDADIISY
                     AAGHKAMGLYAMWALRNELVRIAQPGLLPSEKMQLRLEDLLG"
     gene            858..2471
                     /locus_tag="P5079_11135"
     CDS             858..2471
                     /locus_tag="P5079_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30573.1"
                     /translation="MPHALKQKKRHLLRIIIPAYPAFNIYSFLAHKTTALGPVCVATA
                     AKEVPGWDVEVIDENNLRLFGPRDNAGGTDHALLERLRPADVVGFYGGLTSTIPRLYQ
                     LARFYKEAGAITLAGGQHFVDETLDEALSSGLDYVAIGEGEETIKEFLGALGNHGGFN
                     RVRGLAWRDDKKIVRTPPREPLTDFDGLPLPDFSLLRYAKVKIFPVGRIRGCAMHCEF
                     CTVKSHLRFASPERLLENYRLLVETKNARHFFIVDDLFGQQRNETIRFCRLLQDYQAT
                     INRKLNTTVQIRLDKAKNPELLTAMRHAGIHCVAIGFESPVEEELLAMNKHLRPQDMI
                     SLARTYHDFGFLVHGMFIFGYPLQEGRTLRLSVGERIRQTKDFIRKAKIDTIQVLLPV
                     PLPGTELRARLQDRVFLRKDMGWEYYDGNFPLFEPTPPLTPEDMQLAVRKLMGKFYEF
                     KYMFYIAMKIFLFPGLVLPWFNIKSGWARWYRNWRNYLIRFGGWITIRGWAEKIHKDN
                     FLQKLDTARKHLGARTSQAAGPHKGPPPA"
     gene            2766..>3047
                     /locus_tag="P5079_11140"
     CDS             2766..>3047
                     /locus_tag="P5079_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30574.1"
                     /translation="MADNRNTGTAPFPFHKVWTILLLAGLLSVSPGPLRGAPTDSTSL
                     PADPRKQRHQVGIDLGSDADPDLLAPTAPVGLDRLLEWDYRFVVRDDRIG"
BASE COUNT          665 a          963 c          837 g          582 t
ORIGIN      
        1 ccgagaaggt cctccagccg gagttgcatc ttttccgacg gcaggaggcc gggctgggcg
       61 atgcgcacca gttcgttgcg gagcgcccac atcgcgtaga gccccatcgc cttgtgaccg
      121 gcggcgtagg agatgatgtc ggcgtcgttg cgcagcgggt cggaaaaatc ataactgagc
      181 gcacgataga ggagcccctc cacgaagcgg ccggacgaga tggacccgcc gggatgcccc
      241 gaggtcggca cgaaattgta gagaacgccg cacagcgtgc ggtaggccag atcgagcctt
      301 tcgaaaaacg ggacttcttc ggcgccgagg gccggcggga gggccgtcat ggaatcggcg
      361 atgttgtagt aagcgccgcg gcgcgacggc catttcattt cctgcggttt cacagactcg
      421 atgtcggaca ttgtccctcc tggaagtatc ggctagaaaa aattgggggt tgcttgcggg
      481 gccgggacag aacccgtccc gccggacgtc gttcgctcat tatatcaaaa gaacatttgt
      541 tcggcgggaa agcggatggc ttatccggaa cccgcaaagc gacggatccc ctttcacgga
      601 aaagcggccc cttccggaaa aacctcccga cccatctgcc gtttccggct caactttaaa
      661 aaataacggc cgatgtgagt cgacgcgtat ttgatacaat gaacataagc cattcgaggg
      721 ggcctgtcga tatcgcgccc gccacaattc cttcttgctg ccactcgacc tcctcggcaa
      781 aacaccgcgt tctgaattcg ccgccgcatt gtccgttacc ccgccgactt cgggataaat
      841 tttcacaagg atttcatatg ccacacgcct taaaacaaaa aaaacggcac ctattgcgga
      901 tcatcatccc cgcctacccc gctttcaaca tctattcctt cctggcccat aagacaacgg
      961 ccctggggcc ggtgtgcgtg gccacggccg ccaaagaagt gccgggctgg gacgtcgagg
     1021 tcatcgatga aaacaacctg cggctcttcg gcccgaggga caacgccggc gggacggacc
     1081 atgccctcct ggagcgcctg cggcccgcgg atgtcgtcgg tttttacggg gggctcacga
     1141 gcaccatccc ccgattgtat cagctggccc gtttttataa ggaggcgggc gcgatcacgc
     1201 tggccggggg gcagcatttc gtcgatgaga cattggacga agccctttcc tccggcctcg
     1261 attatgtggc catcggcgaa ggagaagaaa ccataaagga atttttaggg gccctgggaa
     1321 accacggggg attcaaccgc gtgagagggc tggcctggcg ggacgataaa aaaatagtcc
     1381 gcaccccgcc ccgggaaccc ctgacggatt tcgacggcct gcccctgccg gatttttccc
     1441 ttctgcgcta cgccaaggtg aagatattcc ccgtcggtcg catacgcggg tgcgccatgc
     1501 actgcgaatt ttgcaccgtc aagagccatc tccgcttcgc ctcaccggaa cgccttctcg
     1561 aaaattatcg gctcctcgtc gaaacgaaaa atgcccggca ctttttcatc gtcgacgatc
     1621 tcttcgggca acagcggaac gagacgatcc ggttttgtcg cctgctccag gactaccagg
     1681 cgacgatcaa ccggaaactg aacaccaccg tccaaatccg cctggacaaa gcgaaaaacc
     1741 ccgaactcct cacggccatg cggcacgccg ggattcactg cgtggccatc gggttcgaat
     1801 cgcccgtgga agaagagttg ctcgccatga acaaacatct ccgcccccag gacatgatct
     1861 cgctcgcgcg gacgtatcac gacttcggct ttttggtgca cggcatgttc atattcggct
     1921 atccgctcca agaagggcga acgctccggt tgtccgtcgg agaacgcatc cggcaaacaa
     1981 aggatttcat ccgaaaagcc aagatcgaca ccatccaggt ccttttgccg gtccccctgc
     2041 ccgggacgga actccgggcc cggctccaag accgcgtttt cttacgaaaa gatatgggct
     2101 gggaatatta cgacggcaat ttccctcttt tcgaacccac gccgcccctg acccccgaag
     2161 acatgcaatt ggccgtccga aaactgatgg gcaagtttta cgaattcaaa tatatgttct
     2221 acatcgcgat gaagatcttt ctgttccccg gactggtcct cccctggttc aacatcaaat
     2281 ccggctgggc gcgctggtat cggaactggc gcaattatct cattcggttc ggcggctgga
     2341 tcaccatccg cgggtgggcc gaaaaaatcc ataaagacaa tttcctccaa aaactggaca
     2401 cggcgagaaa acatctcggc gcaagaactt cacaggcggc gggaccccac aagggaccgc
     2461 cccctgcctg aaaagccccc gttgccctgg gaaccgttgc cggcggaaac ccaatgggcc
     2521 cgctggaaat ggaactcggc cggagtgcct ggaaaattcg gattgcctca tttcatcgga
     2581 ccgtccccgc tggaatgcgc gacgaccggc cgggatttca atccacgcaa aaaaaagggg
     2641 gctttcgttt caatgaaatc gccccggccg ccccgcggcg gcccagggaa gcggattttc
     2701 cacccatccc tccgaaattc cgcacgctct cctgacaaac ccatcgacta attctaaaat
     2761 acggcatggc cgataaccga aacacaggga ccgcaccgtt cccgtttcat aaggtctgga
     2821 cgatcctcct cctggccgga ctcctttccg tgtcccccgg cccgctccgc ggcgcaccga
     2881 cggactcgac gtcccttccg gccgacccgc ggaaacaacg ccatcaagtg ggtattgatc
     2941 tcggatcgga tgccgacccc gacctcttgg cgccgacggc gcccgtcggg ctcgaccgcc
     3001 ttctcgaatg ggactaccgc tttgtcgttc gagacgaccg gatcggc
//
LOCUS       DAOTFD010000278         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25215_length_3038_cov_2.862554, whole genome shotgun sequence.
ACCESSION   DAOTFD010000278 DAOTFD010000000
VERSION     DAOTFD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25215_length_3038_cov_2.862554"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1871
                     /locus_tag="P5079_11145"
     CDS             78..1871
                     /locus_tag="P5079_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY30575.1"
                     /translation="MKIRYLLLAGLGLLLLPGRLRADFEDLGAGARVFGMGGAFVGVA
                     DDVWAVYYNPAGLGFLRQKQLGADYSRFYVGMSDDSGIGLGFLSYAHPLRKSERRRIP
                     ESVLPSTATAPTVVGSSTTVGVSPSTGPAAGGKAVFPAYGTLAVGLRTFSLSGVYREE
                     TGYLSYGKAVTSHLALGLGLKYMQEIYDQDPYTRIDPVFDYGRKDGIKAFGADAGVLW
                     NPHARFFVGASILDLNRPDVGLKDKEPLPSVYKSGLGYRDKRTILAFDVTSRAKQLRL
                     GVGGERWFQRNAWCLRTGMEYGKEETFSMTLGFALNMAVFQVNYGFRFPLSGVDETAG
                     SHRVSLQMRFGALPKDELDPSSLEYSYLELKQEKNVLESKLQKTEAEKQRLEEVLLEE
                     ATQRIRERIMSAKKSAGPVSGASAGPAGESSLEFVTHYAQEGDTLQALAERYYGDSRK
                     WKEIYEANKDKLGRGGRIKPKTVLLIPHASPAAAPRAVAANHPAPAVKDETPRPPSPV
                     AVLKPTVPEKPAVPAPRPADPVPAAAPPPKASTAGTPKTRVHVVADGDTLKNIAERYY
                     GDSKRWVDIYNANKDKVVRGTAAPGETLTIP"
     gene            1896..>3038
                     /locus_tag="P5079_11150"
     CDS             1896..>3038
                     /locus_tag="P5079_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30576.1"
                     /translation="MSTWEEIQNKIKNAERFGMDETLPRSLDTLSVADYWQRRFEEEH
                     RLLEKSIESKEKEMDFLKERVERQRTELQLTRAELESFRLQLFDKQRYWEERHRSLEL
                     ENQGLKEKISWEVQVRVLEEQNKFLTEHGARKEKASEEDVVARNKWRETETARVQLAS
                     ELQSANEKLNALLSEKKKWEEERTQKGEAMEALNKEKTALEEKLQGLSGASAPLQAEN
                     ERLAKSLADATRTLARLDKHWSTREQDYLLNMEDLARGFAHRVRNYLGIMSGTLQLAL
                     SGEKMEESLKEQILLVDQNAQEMLKTIEEFLSLSRIPEMSLETLDLNQYLRQTVSALE
                     EKCSAAKIKIEWEMAENLPSVRVDTRLMGEAVSAVVLNAVEAMPDGG"
BASE COUNT          646 a          843 c         1039 g          510 t
ORIGIN      
        1 ctatcagatc accagcgacg gaaagaccct gaacggcacc atcgttttgg cgagatgata
       61 tgaagacacg gcgaacgatg aaaatccgat acctcctcct ggcggggctg gggcttctct
      121 tgctccccgg ccggctccgg gcggattttg aggacctggg cgcgggggcc cgggtgttcg
      181 gcatgggggg cgcgttcgtc ggtgtcgccg acgacgtgtg ggccgtctat tacaacccgg
      241 ccgggctggg gttcctccgg cagaaacagt tgggcgcgga ttattcgcgc ttttacgtcg
      301 gcatgtcgga cgacagcggg atcgggctcg gctttttgtc ctacgcccac cctctccgga
      361 aatccgaacg gaggcggatt ccggaatccg tgcttccctc caccgcgacg gcgccgacgg
      421 tggtcggttc gagcacgacg gtcggcgttt cgccgtccac cggcccggcg gcgggaggga
      481 aggccgtttt cccggcgtac ggcaccctcg ccgtggggtt gcgcaccttc agcttgtcgg
      541 gcgtttatcg ggaagaaacg ggctatctgt cttatggaaa agccgtcacc tcccacctgg
      601 ctttggggtt ggggcttaag tatatgcagg aaatctacga tcaggatccc tacacccgca
      661 tcgaccccgt ctttgattac ggccggaaag acgggatcaa ggccttcggc gcggacgcgg
      721 gcgtcctctg gaacccgcac gcccggtttt tcgtgggggc ctccatcctg gacctcaacc
      781 ggcccgacgt gggactgaaa gacaaggagc cgctgccctc cgtgtataaa agcgggttgg
      841 gctaccggga caagcggacg attttggctt tcgacgtcac ctcccgggcg aaacagcttc
      901 gattgggcgt cggcggagaa aggtggttcc agcggaacgc gtggtgtctc cggaccggca
      961 tggaatacgg caaggaagag actttttcca tgacgttggg tttcgccttg aacatggccg
     1021 tttttcaggt caactacggt ttccgctttc ccctgtccgg cgtggacgaa accgccggga
     1081 gccatcgcgt ttccctgcag atgcgtttcg gggcgctccc caaagatgag ttggatccca
     1141 gcagcctgga atattcctat ctggaactga aacaggaaaa gaacgtgttg gaaagcaaat
     1201 tgcagaagac cgaggcggaa aaacaaaggt tggaggaggt cctgctggaa gaggccaccc
     1261 agcgtatccg ggagaggatc atgagcgcta aaaaatcggc cggcccagtc tccggcgcct
     1321 ccgccggtcc ggcgggggag tcctcgctgg agttcgtcac ccactacgcg caggaggggg
     1381 acacgctgca ggcgttggcg gaacgatatt acggggattc gaggaagtgg aaggaaatct
     1441 acgaggcgaa caaggataaa ttgggccggg gcggacggat aaaacccaaa accgtcctgt
     1501 tgattccgca cgcgtctccg gcggcggcgc cgcgtgcggt tgcggcgaac cacccggcgc
     1561 cggccgtgaa agacgagacg ccgcgcccgc cgtccccggt ggcggtgttg aaaccgaccg
     1621 ttcctgaaaa accggcggtc ccggcgccgc ggcccgccga ccccgttccg gccgccgccc
     1681 cgccgccgaa ggcgtcgacg gccgggacgc cgaaaacgcg tgtccatgtg gtcgccgacg
     1741 gggatacgct gaaaaacatc gccgaacgtt attacggcga ttcgaagaga tgggtggata
     1801 tctacaacgc gaataaggac aaggtggttc gaggcacggc tgctccaggg gaaacgttga
     1861 ccatcccctg agaatgttcg acgaggagga caccaatgag cacatgggaa gagattcaga
     1921 ataagatcaa gaacgccgag cggttcggga tggacgagac gctcccgcgt tcgctggaca
     1981 cgctgtcggt ggccgactat tggcagaggc ggttcgagga ggaacaccgg cttttggaga
     2041 aaagcatcga gagcaaggaa aaggagatgg acttcctgaa agagcgcgtc gagcgccagc
     2101 gcacggagtt gcagctgacc cgggcggagc tggagtcctt ccggctgcag ttgttcgaca
     2161 agcagcgata ttgggaagaa cggcaccgct ctctggagct cgagaaccag ggcctcaagg
     2221 agaagatcag ctgggaggtg caggtccgcg tgctcgagga acagaacaag ttcctgacgg
     2281 aacacggcgc gaggaaggaa aaggcctcgg aggaggacgt cgtcgcccgg aataaatggc
     2341 gggagacgga aacggcccgc gtccagctgg catcggaatt gcagagcgcc aatgagaaac
     2401 tgaacgccct gttgtcggaa aagaagaagt gggaggagga gaggacccaa aagggcgagg
     2461 ccatggaggc gttgaacaag gaaaaaacgg cgctggagga gaagcttcag ggcctgtccg
     2521 gcgcgagcgc tcccctgcag gcggaaaacg agcgtttggc gaaatccttg gccgacgcga
     2581 cccggaccct ggcccggctg gacaaacatt ggtccacccg ggagcaggat tatctgctca
     2641 acatggagga cctggcgcgc ggtttcgccc accgggtgcg gaattatctc gggatcatga
     2701 gcggcacgct tcagctcgcc ctgtccggag agaagatgga ggagtcgctg aaggaacaga
     2761 tcctcctggt cgaccagaac gcgcaggaga tgttgaagac gatcgaggag ttcctgtcgt
     2821 tgtcccgcat cccggagatg agcctggaga cgctcgactt gaaccagtat ctgcggcaga
     2881 cggtctccgc cctggaggag aaatgcagcg ccgccaagat caagatcgaa tgggagatgg
     2941 cggagaatct gccgtccgtg cgggtggata cccggctgat gggggaggcg gtgtccgccg
     3001 tcgtgctgaa cgcggtggag gccatgcccg acggcggg
//
LOCUS       DAOTFD010000279         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25445_length_3019_cov_3.735155, whole genome shotgun sequence.
ACCESSION   DAOTFD010000279 DAOTFD010000000
VERSION     DAOTFD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25445_length_3019_cov_3.735155"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="P5079_11155"
     CDS             complement(<1..1245)
                     /locus_tag="P5079_11155"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRY30577.1"
                     /translation="MPARDWPDRLRKLKAAGFNAVSTYIPWIWHEPEEGRTDFSGDTA
                     PERNVLRFIDLAGELGLLCVARVGPVSNAELKNEGIPVWLRETYPDVFVTGHRDVGTL
                     PHVTMLSYLHPVFQEKVSRWYGSLLPHIAPRQVTRGGNVVGVQLCNEVAMVHWLQKGA
                     DRKEHVQRMYRAFLREKYGDVASLNRAHRARHSSFDAVVQPSGEGVELKNLAPHLDWA
                     LFYRRYYAAYFLSLSARAKKFGVDVPLYCNIPQFYDYDIRGRGNYAPMTTSMFRDFPL
                     LTPQLVFGGAYQMRHLDFENFHDVALTTEITRLLSTVTAKDGPPAPAEGETLPALPEF
                     KVRTDNTVPIVCAELQTGIMRDRPRLYPTQVSLNVKTSVGEGLAGINAYMFAGGENPP
                     GLGAFGTYHDWQAPVGPKGEIKP"
     gene            complement(1375..2433)
                     /locus_tag="P5079_11160"
     CDS             complement(1375..2433)
                     /locus_tag="P5079_11160"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRY30578.1"
                     /translation="MNSKVSAALIAACLMFGNSFGWAAKKPAAKAAPKAADAGLTNAV
                     LVDDFEAGWNSASHETTTVALEAAPGRTGNGQKITYDLKDTKQWVAVSKPLTLGNFSG
                     KALEFWVKGKGKNNVLEIKVMDGDGSNFGVKKEGLTATPGWTRVVVTNTDLAYWWGGD
                     KVLGPVTELHFAISAADGGAGEVLLDDLRVGAAPNAASLKDGVIDDGESLAGWATSQA
                     EGAKIALASEMDQDEKALAITYSIPENLWVNARKTVNTSVPSNAAITFFMKGTGDPNN
                     VEIKLLDRDESVFGKVLEQAAGNGAWQEIRIPVSELKHLWGGDDQLDTSLIRYVDIAI
                     SGPGGKGKVLVDNLKIAQ"
     gene            complement(2475..>3019)
                     /locus_tag="P5079_11165"
     CDS             complement(2475..>3019)
                     /locus_tag="P5079_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY30579.1"
                     /translation="FAKHRFRGRDKIFSLLLATMMIPGQVTMIPVFLLLKKLGFLNSF
                     LGLIVPAGVNVFGIFLIRQFMMSIPNDLIESARIDGCSEFRIYWSVILPLCKPVLATL
                     GIFTFMGSWNDFLWPLIVMVREDGYTLPVALANLSGQHPTAFGLLMAAAVVVVLPVIV
                     VFLLAQKYVIQGIATSGLKE"
BASE COUNT          504 a          885 c         1038 g          592 t
ORIGIN      
        1 gggtttgatt tcccccttcg ggccgacggg cgcctgccag tcgtggtagg tgccgaaggc
       61 gccgagaccg ggagggttct ctccgccggc gaacatgtag gcgttgatgc ccgcgagccc
      121 ctctccgacg gaggttttca cgttgagcga cacctgggtg ggatagagcc ggggccggtc
      181 gcgcatgatg ccggtctgca gttcggcgca gacgatgggg accgtgttgt cggtgcgcac
      241 cttgaattcc ggcagggcgg gcagcgtctc gccctccgcc ggcgcgggcg gaccgtcctt
      301 cgcggtcacg gtgctgagga gccgggtgat ctcggtggtg agcgccacgt cgtggaaatt
      361 ttcgaaatcc aggtggcgca tctggtaggc gccgccgaag acgagttgcg gggtgagcag
      421 agggaagtcg cggaacatgg aggtggtcat gggcgcgtag ttgccccgtc cgcggatatc
      481 gtagtcgtag aattgcggga tgttgcagta gagcggcacg tccaccccga attttttggc
      541 ccgggcggag agcgagagga aatacgcggc gtaatagcgg cggtagaaga gggcccagtc
      601 caggtgcggc gcgaggttct tcagctccac gccttccccg gagggctgga ccaccgcgtc
      661 gaaggaagag tggcgggcgc ggtgggcccg gttcagagag gcgacgtcgc cgtatttttc
      721 ccggaggaac gcgcggtaca tccgttggac gtgttccttg cggtcggcgc ccttctggag
      781 ccaatggacc atcgccacct cgttgcagag ctggacgccc accacgttgc ctccgcgcgt
      841 cacctgccgc ggcgcgatgt gaggcaggag cgaaccatac catctcgaga ctttttcctg
      901 gaacacggga tggaggtagg agagcatggt cacgtgcggc agggtcccca cgtcgcgatg
      961 gccggtgacg aagacgtcgg gataggtctc cctcagccag acggggatgc cttcgttctt
     1021 cagctcggcg ttggagacgg gacccacccg cgcgacgcag aggagcccca gttcgccggc
     1081 gaggtcgatg aacctcagca cgttccgttc cggcgcggtg tcgccggaga agtcggtgcg
     1141 gccctcctcc ggctcgtgcc agatccaggg gatgtaggtg ctgaccgcgt tgaacccggc
     1201 cgccttcagt ttcctgaggc ggtcgggcca gtctctggcg gggatgcgga aatagtgcgt
     1261 ctcccccgaa tagaggaaga acggctttcc gtccagccaa aacgcgccat ccttcagttc
     1321 aatggatatt cccgccagag acctgccctc ccgttccgtt cgagcgtgcg gagcttattg
     1381 cgcgatcttc aggttgtcca ccagcacctt gcctttgccg ccgggaccgc tgatggcgat
     1441 gtccacgtag cggatgaggg aggtgtccag ctggtcgtct ccgccccaga gatgtttcag
     1501 ttccgagacg gggatgcgga tttcctgcca ggcgccgttg cccgcggcct gttccagcac
     1561 cttgccgaac accgattcgt cgcggtcgag gagtttgatc tcgacgttgt tcgggtcgcc
     1621 ggtgcctttc atgaagaagg tgatggccgc gttcgacggc acgctggtgt tgaccgtttt
     1681 acgggcgttg acccacaggt tctccgggat ggagtaggtg atggcgaggg ctttttcgtc
     1741 ctggtccatt tccgaggcga gggcgatctt ggcgccttcc gcctgggagg tggcccatcc
     1801 ggccagggat tcgccgtcat cgatgacgcc gtccttcagg gaggcggcgt tgggggcggc
     1861 gcccacgcgg aggtcgtcca acaaaacctc gccggctccg ccgtcggcgg cgctgatggc
     1921 gaagtggagt tccgtcaccg gtccgaggac cttgtcgccg ccccaccaat aggcgaggtc
     1981 cgtgttggtc acgacgacgc gcgtccagcc gggggtggcg gtgagcccct ctttcttcac
     2041 gccgaaattg ctgccgtcgc cgtccatgac cttgatttca aggacgttgt tcttgccctt
     2101 gcctttgacc cagaactcca aggctttgcc gctgaaattg ccgagggtca gcggtttgct
     2161 gacggccacc cactgtttgg tgtccttgag gtcgtaggtg attttctggc cgttgccggt
     2221 gcggccgggg gccgcttcga gggcgacggt cgtggtttca tggctggcgc tgttccagcc
     2281 ggcctcgaag tcgtccacga ggacggcgtt cgtcagtccc gcgtccgccg ctttcggggc
     2341 ggccttggcc gccggttttt tggccgccca gccgaagctg ttgccgaaca tcagacaagc
     2401 ggcgatcaat gctgccgata ctttgctgtt catacaagtc tctccttttt tatgaggtga
     2461 aatgatgacg aaaactattc tttgagtccc gaggtggcga tgccctggat gacgtatttc
     2521 tgcgccagga ggaagacgac gatgacgggc agcaccacca ccaccgccgc cgccatcaga
     2581 aggccgaagg ccgtggggtg ctggccgctg aggttcgcca gggccaccgg cagggtgtag
     2641 ccgtcctccc gcaccatcac gatcaacggc cagaggaagt cgttccagga acccatgaag
     2701 gtgaaaatgc cgagggtggc cagcaccggc ttgcacagcg gcaggatgac gctccagtag
     2761 atgcggaact cggagcaacc gtcgatgcgc gccgattcga tgaggtcgtt ggggatggac
     2821 atcatgaact gccggatgag gaagatgccg aacacgttca cccccgccgg cacgatgagg
     2881 ccgaggaaac tgttgaggaa cccgagtttt ttcagcagca ggaacaccgg gatcatggtc
     2941 acctggccgg ggatcatcat ggtggccaaa aggaggctga aaatcttgtc gcggccgcgg
     3001 aagcggtgct tggcgaagg
//
LOCUS       DAOTFD010000280         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25495_length_3016_cov_4.263762, whole genome shotgun sequence.
ACCESSION   DAOTFD010000280 DAOTFD010000000
VERSION     DAOTFD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25495_length_3016_cov_4.263762"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="trpA"
                     /locus_tag="P5079_11170"
     CDS             complement(<1..539)
                     /gene="trpA"
                     /locus_tag="P5079_11170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRY30580.1"
                     /translation="MNRLDRTFSRLRRQKRKALIAYLTAGYPSPKEMDRTARLLEHAG
                     VDVLEVGIPFSDPVADGPTIQYSSQKSLEKGTTLPRVLRWTRRFRKTSSLPVVFMSYL
                     NPIHHYGYARFAKDAAKAGGDGLIVPDLVPEESRALRKLLAVHGLHLIHLVAPTSPPA
                     RQQRIARLTRGFLYAVSLTG"
     gene            complement(536..1732)
                     /gene="trpB"
                     /locus_tag="P5079_11175"
     CDS             complement(536..1732)
                     /gene="trpB"
                     /locus_tag="P5079_11175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRY30581.1"
                     /translation="MLPDRRGYFGPYGGRFVPETLLAPLVEVEEAFRRAQKDPSFRRE
                     LNDLLHRYAGRPTPLFFAKNLSRRLGGAKIFLKREDLCHTGSHKLNNTLGQCLLARRL
                     GKKRVIAETGAGQHGVATATAGALMGLQCAVYMGSEDMARQALNVFRMRSLGTRVVPV
                     NSGSRTLKDAINEAFRDWVATVNTTFYAMGSVVGPHPYPWMVREFQSVIGRETARQIR
                     EYQSGDPDALVACVGGGSNSMGLFAPFYEKRRVKFFGVEAGGKGLMTGRHAATLCAGT
                     PGILHGAYTYVLQDKHGQIRPTHSISAGLDYPAVGPEHSFYRDSGRAEYVAATDSEAV
                     EGFRLLNETEGITAALESAHAIGYLPRLLRGHRSLKSVVVCLSGRGDKDMAQLQDLLP
                     SEGASR"
     gene            complement(1770..2411)
                     /locus_tag="P5079_11180"
     CDS             complement(1770..2411)
                     /locus_tag="P5079_11180"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRY30582.1"
                     /translation="MAVKIKICGITNEEDATWAANLGAHYLGFNFWSQSPRKVSVEMA
                     LRVVEKTPPFVLPVGVFVDHPAADIVKIVKKTRLRGVQLHGEQTPEECGELRNSLDPS
                     VFLCKVFRVAQAADMENAGLYTDSCQYFLLDARVEGEPGGTGQTFPWDLAKQAKSHGV
                     PIFLAGGLTPENVREAIKQAEPFGVDVASGVEKSPKRKDFDKLKSFIEQAKRA"
     gene            complement(2428..>3016)
                     /locus_tag="P5079_11185"
     CDS             complement(2428..>3016)
                     /locus_tag="P5079_11185"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRY30583.1"
                     /translation="RAPYNVAEGAKAYARAGAAALSVLTESVFFQGNISHIGLAKDAA
                     GLPVLRKDFLFDPYQIIEARAHGADAVLLIAAILKDRELLSLIRLAKSLGLAPLVEVH
                     DGNELERALSAEAPIVGVNSRNLKDLTVDLNTFSKLLPKIPDTVLTVAESGIRGPKDV
                     QMLKSLGADAMLVGESLLRRKNMGTAARVLINAGK"
BASE COUNT          524 a          904 c         1010 g          578 t
ORIGIN      
        1 cccgtgaggg aaacggcgta aaggaatccc cgtgtgaggc gggcgatgcg ctgttggcgc
       61 gccggcggcg aggtcggcgc cacgagatgg atcagatgga gcccgtggac cgccaacagt
      121 tttcggaggg cccgcgattc ctccgggaca aggtcgggca cgatcagtcc atcccccccc
      181 gctttcgccg cgtctttcgc gaagcgggca taaccgtaat ggtggatggg gttcagatag
      241 ctcatgaaga cgaccggcaa cgaagaggtt ttccggaacc ggcgtgtcca gcgaagaacg
      301 cggggcaggg tggtcccttt ttccagggat ttctgggaag aatattggat ggtcggtccg
      361 tcggcgacgg gatcggagaa agggatgccc acttcgagga cgtccacgcc ggcgtgttcg
      421 aggagccggg cggtgcggtc catctccttc ggggacgggt agccggcggt gagataggcg
      481 atgagcgcct tgcgtttttg ccgacgcagc cgggaaaagg tccggtcgag gcggttcatc
      541 gggacgcccc ttccgaagga agaagatctt gcagctgcgc catgtccttg tcgcctcggc
      601 ccgagaggca caccacgacg cttttcaggg agcggtgtcc cctcaaaagg cgcggcaggt
      661 acccgatggc gtgggccgat tccagggcgg cggtgatgcc ttcggtctcg ttcagcaggc
      721 gaaacccttc caccgcttcg ctgtcggtgg ccgcgacgta ctcggcccgg ccggaatccc
      781 gatagaacga atgttccggc ccgacggcgg gataatccag gccggcggag atcgaatggg
      841 tcgggcgtat ctggccgtgt ttgtcctgga ggacgtaggt ataggcccca tggaggattc
      901 cgggagttcc ggcgcagagg gtggcggcgt gtcggccggt catgaggcct ttcccccccg
      961 cctccacgcc gaaaaatttc acgcggcgtt tttcgtagaa cggagcgaaa aggcccatgg
     1021 agttggaacc gcctcccacg caggcgacga gggcgtccgg gtcgccggac tgatattccc
     1081 ggatctggcg cgcggtttcc cggccgatga cgctctggaa ttcgcgcacc atccagggat
     1141 agggatgcgg ccccaccacc gaacccatgg cgtagaacgt ggtgttcacg gtggccaccc
     1201 agtcgcggaa ggcctcattg atggcgtcct tcagcgtacg cgaaccggaa ttgaccggca
     1261 cgacgcgggt cccgaggctc cgcatgcgga agacgttcaa cgcctggcgc gccatgtcct
     1321 cggatcccat gtacaccgcg cattgaagtc ccatgagggc gcccgcggtg gcggtggcca
     1381 cgccgtgttg gccggcgccg gtttcggcga tgacccgttt tttccccaaa cggcgggcca
     1441 acaggcattg gcccaaggtg ttgttgagtt tatgagaacc ggtgtggcaa aggtcttcgc
     1501 gtttcaggaa gatcttcgcg ccgccgaggc ggcggctgag attcttggcg aaaaagaggg
     1561 gcgtgggacg gccggcgtaa cggtggagaa gatcgttcaa ttcgcggcgg aacgacgggt
     1621 ctttttgggc ccggcggaac gcctcctcca cttccacgag gggagcgagg agcgtttcgg
     1681 ggacgaaccg gccgccgtag gggccgaaat atcctcgacg gtccggcagc atcagagaga
     1741 ctccttccgg cgaaggccgc gatgagtctt catgcccgtt tcgcctgttc gatgaaggat
     1801 ttcagtttgt cgaaatcttt tcgtttgggg gatttctcca cgccggaggc cacgtccacc
     1861 ccgaacggtt cggcctgttt gatggcctcg cgcacgtttt ccggcgtcag gccgccggcc
     1921 agaaaaatgg gaacgccgtg ggatttggcc tgtttggcga ggtcccaggg gaacgtttga
     1981 ccggtgccgc cgggttcgcc ctccacgcgc gcgtccagca ggaaatactg gcagctgtcc
     2041 gtgtagagac cggcgttctc catatccgcc gcctgcgcca ctcggaagac cttgcagagg
     2101 aagacggacg gatccaatga attgcggagt tcgccgcatt cttccggcgt ctgttcgccg
     2161 tggagctgta cgccgcggag gcgcgttttt ttgacgatct tcacgatgtc cgccgcggga
     2221 tggtcgacga acacccccac cgggagcacg aaaggcggcg tcttctcgac gacgcggagc
     2281 gccatctcga cggagacttt gcgggggctc tgtgaccaga aattgaatcc caggtaatgg
     2341 gcgcccaggt tcgcggccca cgtggcgtct tcttcgttgg tgatcccgca gatcttgatt
     2401 ttgacggcca tagcgactcc ttttctttta ctttccggcg ttgatgagga cgcgggcggc
     2461 ggtccccatg tttttccgcc gcaaaaggga ttcccccacc aacatcgcgt ctgcccccaa
     2521 actcttcagc atctgcacgt ctttggggcc ccggatgccg ctttcggcca cggtcaggac
     2581 ggtgtccggt attttcggaa gcaacttcga aaacgtgttc aaatccaccg tgaggtcctt
     2641 cagattccgg ctgttgacgc cgacgatcgg cgcctcggcg gacaaggccc gttccagttc
     2701 gttgccgtcg tgcacttcca ccaggggggc cagtcccagg ctcttcgcca gccggatgag
     2761 ggacagcagt tcgcggtctt tcaagatggc ggcgatcaac aagaccgcgt cggcgccgtg
     2821 ggcccgggcc tcgatgatct ggtacgggtc gaacaaaaaa tcttttcgga gcaccggcag
     2881 acccgccgcg tccttggcca ggccgatgtg ggagatgttc ccctggaaaa agaccgattc
     2941 cgtgaggacc gacagggccg ccgcgcccgc ccgggcgtag gccttggccc cttcggcaac
     3001 gttgtacggc gcccgg
//
LOCUS       DAOTFD010000281         2994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25758_length_2994_cov_3.444369, whole genome shotgun sequence.
ACCESSION   DAOTFD010000281 DAOTFD010000000
VERSION     DAOTFD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25758_length_2994_cov_3.444369"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2333)
                     /locus_tag="P5079_11190"
     CDS             complement(<1..2333)
                     /locus_tag="P5079_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30584.1"
                     /translation="MPMAQRGVRFFSLVASILLVLPAALSASDQSLGLMSARDRGTFN
                     IGPAQGKADRSADPTLKKDIVKFEFSAPAGSVAGVWTKNYPSTLTAATVSDVKIAVNV
                     PAAGLERQIAVSLELKGTKAAQVVPLSLQAGWNIVQKAVEWGKIGDFKEAVFVVSPAA
                     GTSASGTVYFDLDFIRPVSAPRRGSSTGFGLLDARERGAFNMGAAKGNVALFADDIAK
                     KDVLKLDYSAPQGSAVGLWTKAYPSGLGAASVNAVNVLVNVPSAAQVRQVSLTLELKG
                     TRVTQSIPLPLKTGRNSFQERVEWDKIGDLKEVVFVVSPVGGGRSAAGSLFFDLEFVR
                     LKSAPDRHVSAGTPLAFSVFDARERGVFNMGASKGTLAAAVDPVEKKDVLKFGYTVPQ
                     GTAAGVWIKGYPAELGPGSVNAVKVGVNVPAPDQPRQVSVTLEFKGTRVTQSRPLSLQ
                     PGWNTLQEIINWDMIGDLKEVVFVVTPVGGEKPVSGVFFFTLDFLRVTELPKPAAPAE
                     ATAVAKSCGCALKSLGGIFLIGLLLALGAALVSRLSGRRSSPGRGAESVGLPALDASL
                     LFRLKRDILYGAAATMLAGTALGIYVLGTQPDVFYGVLAVGVAGGLIAEALKFAVAGK
                     HLTPGELFQNVFLTGLLAATSSGQVLWQAPATWTEVLMKSNLTVALACLAYHVANACS
                     LFNSGKHLRPTIGALIVGTPYLFGWLLMLENVGLLRALGFGVAVGTFAHGPAVSEALG
                     RLLVVFAFNEAVTALISLATKGRLLKTPKAHLYALLVS"
     gene            complement(2520..>2994)
                     /locus_tag="P5079_11195"
     CDS             complement(2520..>2994)
                     /locus_tag="P5079_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30585.1"
                     /translation="LPFSRITVLAAVGLKIDTSHKVKVQQGPSPAEKAVNMGVMMMTG
                     LPLGIGKGKKEAVKNEVSSELFFYLDLLVRGPEAHYRVDAQHLDYAFLRDEKTFNVAG
                     NFRVLSGKIAERSPAALKNKGLRAILGGGNLTTLGYDSMEDFERELHWLAQLGDS"
BASE COUNT          641 a          988 c          866 g          499 t
ORIGIN      
        1 gaaaccagga gcgcgtagag gtgggccttg ggtgttttca ggagccggcc tttggtggcc
       61 agactgatga gcgcggtgac cgcctcgttg aaagcgaaca ccaccaacaa ccgccccaaa
      121 gcctccgaga cggccggacc gtgggcgaac gtcccgacgg caaccccgaa gcccagcgcc
      181 cgcaacaggc ccacgttctc cagcatcaac agccagccga acagataggg cgtcccgacg
      241 atcaacgccc cgatggtcgg ccgcaaatgt tttccggaat tgaacaggga gcaggcgttg
      301 gcgacgtgat aggccagaca cgccagcgcc acggtgaggt tgcttttcat cagcacttcc
      361 gtccaggtgg ccggcgcctg ccacaacacc tggccgctgg aggtggccgc caacaggccc
      421 gtcagaaaga cattttgaaa aagttctccg ggagtcagat gtttccccgc aacggcgaat
      481 ttcagcgcct ccgcaatgag gcccccggcc acgccgacgg ccaacacacc gtaaaaaacg
      541 tccggctgcg ttcccaaaac ataaataccc agcgccgtcc ccgccaacat cgtcgccgcc
      601 gcgccgtaga gaatatcccg tttcaaacgg aaaaggagag aggcgtccaa agcgggaagt
      661 ccgacgctct ccgccccccg gccgggggag gaccgtcgac cggacaaacg actcacgagg
      721 gccgccccca acgccaacag gaggccgatc aaaaagatcc cgccgagact cttcaacgca
      781 cagccacaac tcttggccac ggccgtggcc tcggccgggg ccgccggctt cggcagttcc
      841 gtcaccctga gaaaatcgag ggtgaaaaag aagacgccgg aaaccggctt ctccccgccg
      901 acgggcgtca ccacgaagac gacctccttc aggtcaccga tcatgtccca gttgatgatt
      961 tcctgaaggg tgttccagcc gggctgaagg gagagcggcc ggctctgcgt cacccgcgtg
     1021 cccttgaact ccagagtgac gctgacctgc ctgggttggt ccggggcggg cacgttgacg
     1081 ccgaccttca ccgcgttgac ggaccccggg cccagttcgg cgggataccc tttgatccaa
     1141 acgccggcgg cggtcccctg gggcaccgta taaccgaact tcaaaacgtc ttttttctcg
     1201 accgggtcga ccgcggccgc gagggtcccc ttggaggcgc ccatgttgaa caccccgcgt
     1261 tcgcgggcat cgaacacgct gaacgcaaga ggcgtcccgg cggaaacatg gcggtccggc
     1321 gcggatttca accggacgaa ctccaggtcg aagaacaagg aaccggcggc ggaacggccc
     1381 ccccccaccg ggctgacgac gaacaccacc tccttcaggt ccccgatctt gtcccattcc
     1441 acgcgttcct gaaaggaatt gcgtccggtc ttgagcggca aggggatgct ctgcgtgacc
     1501 cgcgtgccct tgagttccaa cgtcagagaa acctgccgca cctgggcggc gctgggcacg
     1561 ttgaccagaa cgttgaccgc gttgacggag gccgccccca acccggaggg ataggctttc
     1621 gtccagaggc cgaccgccga cccctgcggc gccgaatagt cgagtttcag aacatctttc
     1681 ttggcgatat cgtcggcaaa aagagcgacg tttcccttgg ccgcgcccat gttgaaagcc
     1741 ccccgctcgc gggcatccaa caggccgaag cccgtcgagg aaccccggcg gggcgcggac
     1801 accggcctga tgaaatccag atcgaaatac accgtgccgg aggccgacgt ccccgccgcc
     1861 gggctcacga cgaaaacagc ctctttgaaa tccccgattt tcccccactc gaccgctttc
     1921 tgaacgatat tccagccggc ctgaagcgac aggggaacga cttgggcggc tttcgtcccc
     1981 ttgagctcca gcgacacagc gatttggcgt tccagcccgg cggccggcac gttcacggct
     2041 atcttgacgt cggacaccgt ggcggcggtc aaggtcgaag gataattttt cgtccacacg
     2101 cccgcgaccg aacccgcggg cgccgaaaac tcgaacttca ctatgtcttt cttgagggtc
     2161 ggatcggccg aacggtcggc cttcccctgc gccggaccga tattgaacgt cccccggtca
     2221 cgggcgctca tgagaccaag cgattgatcc gaggcggaaa gggcggcggg aaggaccaac
     2281 aggatggaag cgacaagaga aaaaaagcgg acacctcgct gcgccatggg catgctcatc
     2341 ctttttaaaa ccggccggcc gcaagacgga ttcttcgtcc gcggcaagga cggcgatatt
     2401 tttggaaaag atttaacctg aaatttcaaa ttgcggaaag gaaacgactc cgccaataat
     2461 agaaaatttt gcggacgctt atgcttcttt tttaccgagg aaaccggtcc gggatcggct
     2521 caggaatccc caagctgggc cagccaatga agttcccgct caaaatcctc catcgaatca
     2581 taccccagcg tcgtaaggtt cccgcctccc aggatggcgc ggagcccctt attcttcagg
     2641 gccgcgggtg accgctcggc gatttttccc gacaaaaccc ggaaattgcc ggccacattg
     2701 aaggtttttt cgtcccgcag gaaggcgtag tccagatgtt gcgcatccac gcggtaatgg
     2761 gcctccggcc cgcggaccag caggtccagg taaaaaaaga gctccgagga gacctcgttc
     2821 ttcaccgcct cctttttccc cttcccgatg cccagaggga gccccgtcat catcatgacc
     2881 cccatgttca cggccttctc ggcgggcgac ggtccctgct gaaccttcac cttgtgggag
     2941 gtgtcgattt tcagtccgac cgcggccaac accgtgatgc gggaaaaagg caac
//
LOCUS       DAOTFD010000282         2986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_25843_length_2986_cov_4.078472, whole genome shotgun sequence.
ACCESSION   DAOTFD010000282 DAOTFD010000000
VERSION     DAOTFD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25843_length_2986_cov_4.078472"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="P5079_11200"
     CDS             complement(<1..317)
                     /locus_tag="P5079_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30586.1"
                     /translation="MIRINLLPPEIHVAEVRKQAAIAGGILGSLLVMSMLSYWGTRVM
                     KVRRMEKDLALAQEELRKYQAIVDKVTQLEQTRNQLQARRDVIKQLVKGGLLYPKFFE
                     DFMA"
     gene            complement(314..1447)
                     /gene="pilM"
                     /locus_tag="P5079_11205"
     CDS             complement(314..1447)
                     /gene="pilM"
                     /locus_tag="P5079_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRY30587.1"
                     /translation="MGLADKLKNVRLEKFRFFKAKETIGVDLGSTSIKLIQLRGSPGR
                     WSLARWAYLPLPNASPDVPGPERKTQATNLLRDYVFKQKKTAVKSAAFAVSGNSVIVR
                     YVKFPKMSRDDLSKTIQFEAEPYIPFAIPEVNIDFHILGDVVEDGQKKMETILVAAKK
                     EIIGSRLDVIRQAGLNPIVIDVDAFSLQNAYDVNRNPGSKETVLIVHIGASVTTMAIV
                     ETGVPKVVRDVFIAGNTLTKALQRNFQCDAKAAEEMKSRAVLLATPEEREKAVAEENK
                     ELLQMSTVMLPVVKDLLAEVQRSIDFYLSQGSDRQVARVLLSGGGCRLGNLVAYFNHE
                     LRLPVELFDPLARIEGAKAISPEMRPLFAVAVGLALRRDGDNL"
     gene            complement(1609..2388)
                     /locus_tag="P5079_11210"
     CDS             complement(1609..2388)
                     /locus_tag="P5079_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRY30588.1"
                     /translation="MLLFLWILLALILGSFANVCIHRLPRGESIFWPPSRCPSCGKNI
                     RWWQNVPLLSFLLLRGRCAACQKPIAWRYPLVEFLTALLFLALFLRFPERAVPLWIGA
                     TLSLLLLILTFIDLEHQIIPDALSLAFLVGGLAAFPVNPLLGSTVLLRGLTALTGAAT
                     GLALLWGIALVGKMVWHKEAMGGGDIKFMAALGTLLGWKGILFSLFTGSLLGSVWIGL
                     TFWKRRPGEYLPFGPFLAMGAWLCWYLYAGNGIPWPLLKTF"
     gene            complement(2443..>2986)
                     /locus_tag="P5079_11215"
     CDS             complement(2443..>2986)
                     /locus_tag="P5079_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY30589.1"
                     /translation="RESNYKDGRGGNTSSPVFTFLPMDISRRSSAAPAGGFTLIELMI
                     VVAVVAVIAAIAIPKFGELISRSQESSAKGALGTFRSALDIYYVANQGMFPSDINALT
                     QGKLIDFLPKIQLPAVALQGNPGHGASNSVENYTAENLFGTVNEGDNMWAYVNDGLDQ
                     GHVGFNCIHKDTKGTVWSDQ"
BASE COUNT          602 a          809 c          986 g          589 t
ORIGIN      
        1 gccatgaagt cctcgaaaaa tttcggatag aggagcccgc ccttgacgag ctgcttgatg
       61 acgtcccgcc gggcctggag ctggttgcgc gtctgctcca gctgggtcac cttgtccacg
      121 atggcctgat atttgcgcag ttcctcctgc gccagggcca gatctttttc catccggcgg
      181 accttcatga cgcgcgtgcc ccaatacgac aacatggaca tcaccagcag gctccccagg
      241 atgcccccgg cgatcgccgc ctgtttccgc acctcggcga cgtggatttc gggaggcagc
      301 aggttgatgc gtatcatagg ttgtccccgt cccgccggag ggccaggccg acggccaccg
      361 cgaagagggg ccgcatttcc ggcgagatgg ctttggcgcc ttcgatgcgg gccagcgggt
      421 cgaagagctc gacgggcagg cgcagttcat ggttgaaata ggcgacgagg ttccccagcc
      481 ggcagccgcc gccggagagc aggacgcgcg ccacctgccg gtcgctgcct tgggagagat
      541 aaaagtcgat ggagcgctgc acctccgcca ggaggtcttt gaccacgggc agcatgaccg
      601 tggacatctg caggagttcc ttgttctctt cggccacggc cttttcgcgc tcttcgggcg
      661 tggccagcaa gacggcgcgt gacttcatct cctcggccgc cttggcgtcg cactggaaat
      721 tccgttggag ggccttggtg agggtgttcc cggcgatgaa cacgtcgcgg accactttcg
      781 ggacgcccgt ctccacgatg gccatggtgg tgacggaggc gccgatgtga acgatgagga
      841 cggtttcttt ggagccggga ttgcggttga cgtcgtaggc gttctgcagg gagaaggcgt
      901 cgacgtcgat tacgatggga ttgagccccg cctggcggat gacgtcgagc cggctgccga
      961 tgatctcctt cttggcggcg acgaggatgg tctccatctt tttctggccg tcttccacca
     1021 cgtctccgag gatgtggaag tcgatgttga cttccgggat ggcgaagggg atgtagggtt
     1081 ccgcctcgaa ctggatggtt ttggaaaggt cgtcgcggga catcttgggg aacttcacat
     1141 aacgcacaat gaccgagttc cccgagacgg cgaaggcggc ggatttgacg gcggtctttt
     1201 tttgcttgaa gacgtaatcc cgcaggaggt tggtggcctg ggttttgcgt tccggcccgg
     1261 gcacgtccgg gctggcgttg gggagaggca ggtaggccca tcgggccaac gaccagcggc
     1321 ccggggatcc ccggagttgg atgagcttga tggaggtgga tcccagatcc accccgatgg
     1381 tttccttggc cttgaaaaac cggaatttct caagacggac gtttttaagt ttgtcggcca
     1441 accccatagt cgctcttatt tacaatatat tttcttactc cgtcaagcaa aaacgccgag
     1501 ctttcccctt aacatggggg gtttgctcgg cgttttcagc atgaattcga cgttcctatt
     1561 ataagccaag gaaattgttt gtcaagggtt tttaaataaa aaataaaatt aaaacgtttt
     1621 taataggggc caaggaatgc cgttccccgc gtaaaggtac caacacagcc aggcgcccat
     1681 cgccaaaaaa ggcccgaaag gaagatattc tcccggccgg cgtttccaga aggtcagtcc
     1741 gatccagacg gaccccagga gggaccccgt aaaaagagag aacagaatcc ctttccagcc
     1801 cagaagggtt ccgagggcgg ccatgaattt gatgtccccc ccgcccatcg cctccttgtg
     1861 ccagaccatc tttccgacca acgcgatgcc ccacaggagg gccagccccg tggcggctcc
     1921 cgtcaaggcc gtcaggccgc gcaggaggac ggtggaaccc agaagagggt tcaccgggaa
     1981 ggcggcgagg ccgccgacga ggaacgccag ggagagggcg tcggggatga tctgatgttc
     2041 cagatcgatg aacgtcagga tgaggagcag gagagaaagc gtcgcgccga tccacagggg
     2101 gacggctctc tcgggaaagc ggagaaaaag ggccaggaag aggagggcgg tcaggaactc
     2161 caccagaggg tatcgccacg cgatgggttt ttggcaggcg gcacatcggc cgcgcaacaa
     2221 gagaaagctc aacagcggga cattctgcca ccagcggatg tttttcccgc aggaagggca
     2281 tcgggacgga ggccagaaaa tggattctcc tcgcggcagg cggtggatgc agacgttggc
     2341 gaaactgccc agaatcaggg cgagaagtat ccagagaaac aaaagcatgg aggtccggtc
     2401 agaggggccg ggagaacgat tccgaagggc gggggacgga ggctactggt cgctccaaac
     2461 ggtgcctttc gtgtctttgt ggatgcagtt gaagccgacg tgcccctggt cgagtccgtc
     2521 gttcacatag gcccacatgt tgtcgccttc gttgacggtg ccgaagaggt tttcggccgt
     2581 gtagttctcg acggaattgc tggcgccgtg tcccggattg ccctggagcg ccaccgccgg
     2641 aagctggatt ttgggaagga agtcgataag ttttccttgg gtgagggcgt tgatgtccga
     2701 ggggaacatg ccttggttgg ccacgtagta aatgtcgagg gcgctgcgga acgtgccgag
     2761 ggcccctttg gcgctggatt cctgagagcg ggagatgagc tcgccgaatt tggggatggc
     2821 gatggcggcg atgaccgcca cgacggcgac gacgatcatc agttcgatca aggtgaagcc
     2881 gccggcgggg gcggcggaac ttcggcggga gatatccatt gggagaaaag taaagacggg
     2941 agaggaggtg ttcccccctc tcccgtcttt gtaattcgat tccctc
//
LOCUS       DAOTFD010000283         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_26412_length_2942_cov_3.932456, whole genome shotgun sequence.
ACCESSION   DAOTFD010000283 DAOTFD010000000
VERSION     DAOTFD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26412_length_2942_cov_3.932456"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="P5079_11220"
     CDS             complement(<1..308)
                     /locus_tag="P5079_11220"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HRY30590.1"
                     /translation="MKPLGIIAGNSQFPLLVAEEAKRQGRAVVAAAIREETDPALEKR
                     VDRLEWFSLGQIKKTLAFFRDAGVEEAVMAGQVKHTQLFRRLKLDLTAVKLLATVKDK
                     "
     gene            complement(349..1185)
                     /gene="lpxA"
                     /locus_tag="P5079_11225"
     CDS             complement(349..1185)
                     /gene="lpxA"
                     /locus_tag="P5079_11225"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRY30591.1"
                     /translation="MTETHSPHPAHIHPTAIVDPSAKIGEGVVIGPYAVIGPDVQIAP
                     HVSIGAHAIVEHARLGTHCKIHPHAFVGTPPQDLKYKGEKTHVRIGAHTVIRECVTVN
                     RGTTASGETVVGENCLLMAYSHVAHDCVVGNNVIMANVATLAGHVHVEDSAVLGGLVA
                     IHQYVRIGQGAMLGGGSMVAQDVAPFCMTQGDRAVIVGLNVVGLRRRGLSPESLSALK
                     NAYKIAFLSGLNLPEAVAQIEKHHMPKEVRLFADFLKKPSHGLCRPPVKIIEKDLEAE
                     GA"
     gene            complement(1198..1638)
                     /gene="fabZ"
                     /locus_tag="P5079_11230"
     CDS             complement(1198..1638)
                     /gene="fabZ"
                     /locus_tag="P5079_11230"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRY30592.1"
                     /translation="MLGTTDIMKIIPHRYPILMVDKAVIVEPDKKITGYKSVSGNEPF
                     FQGHFPGQPIMPGVLIVEAMAQAACVLMLSKPELKNKLAFFMGIDKVKFRKPVVPGDQ
                     LELRVEILRGGGRVGKARGEAFVKDEMVTEAEFMFAIIEPQSNA"
     gene            complement(1710..2594)
                     /gene="lpxC"
                     /locus_tag="P5079_11235"
     CDS             complement(1710..2594)
                     /gene="lpxC"
                     /locus_tag="P5079_11235"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRY30593.1"
                     /translation="MPLVPSQEPQRTIGKEAVYKGVGLHTGNRCHLVFKPAPEDSGIV
                     FVRSDLPGKPRLPASYRIVSSVIRGTTLSLGGEKDHEARVHTVEHVLAALSGLGIDNL
                     AIEVNANEPPVADGSARPFIETLRAAGVRDQNKHRTYLRPDPFVYKGGETEYRVEPAD
                     ELVLETTIEFKHPLIQRQNYVFHFSTDAFVREISPARTFCFDYEVEALKRQGLARGGS
                     LDNAVVVGMDKIHNKEKALRFPDEFVRHKTLDLLGDLSLLGYPLLAKITTVRPGHGYN
                     VNLVKQLAAQLETAAINR"
     gene            complement(2599..>2942)
                     /locus_tag="P5079_11240"
     CDS             complement(2599..>2942)
                     /locus_tag="P5079_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRY30594.1"
                     /translation="GRGTKIDNLVQIAHNVEIGEHCILVALTGVAGSVKMGNYVTLAA
                     QVGVAGHLSIGDGVVCGAKGGISHDIKPREVVWGVPAQPLKDELKTLAAVRKLPELLE
                     ELKKLKKKLSL"
BASE COUNT          456 a          851 c         1013 g          622 t
ORIGIN      
        1 tttttgtcct tcacggtggc gagcagtttc acggcggtga ggtcgagttt cagcctccgg
       61 aaaagctggg tgtgtttcac ctgacccgcc atgacggctt cctcgacgcc ggcgtcccgg
      121 aagaacgcga gggtcttctt gatctgtccc agggagaacc attcgagccg gtcgacccgt
      181 ttttccaagg ccgggtcggt ctcctcccgg atggcggccg ccacgacggc gcggccctgc
      241 cgtttggcct cttcggccac caacaagggg aactggctgt tgccagcaat gatgcccagg
      301 ggtttcatgg gaaagggggt ccggggggcc gatggggagg tggacgtctc aggcgccctc
      361 ggcttccagg tctttttcaa tgattttgac gggcggccgg cagaggccgt gggacggctt
      421 tttgaggaaa tccgcgaaga ggcggacctc cttgggcatg tggtgctttt caatctgggc
      481 caccgcctcc ggcaggttga gtcccgagag gaaggcgatc ttgtaggcgt tcttgagggc
      541 gctgagggat tccggagaga ggccccgtcg gcgcaggccg acgacgttaa ggccgacgat
      601 gacggcgcgg tccccctgcg tcatgcagaa aggggccacg tcctgcgcca ccatggagcc
      661 gccgcccagc atggcgccct gcccgatgcg gacgtattga tggatcgcca ccagccctcc
      721 cagcaccgcg ctgtcttcca cgtgcacgtg tcccgccagg gtggcgacgt tggccatgat
      781 gacgttgttc cccaccacgc agtcgtgcgc cacgtggctg taggccatca aaaggcagtt
      841 ttctcccacc acggtctcgc cgctggccgt ggtgccgcgg ttgacggtga cgcattcgcg
      901 gatgacggtg tgcgcgccga tgcgcacatg ggtcttctcc cccttgtatt tcaggtcctg
      961 cggcggcgtg ccgacgaagg cgtgggggtg gatcttgcag tgcgtcccta ggcgggcgtg
     1021 ttccacgatg gcgtgggccc cgatcgagac gtgcggcgcg atctggacgt ccgggccgat
     1081 gacggcgtaa ggaccgatga cgaccccctc cccgattttg gcggaggggt ccacgatcgc
     1141 ggtggggtgt atgtgggccg ggtgcgggga atgggtttcg gtcatgaaag ggggcctcta
     1201 ggcgttgctt tgaggttcga tgatggcgaa catgaattcc gcttccgtga ccatctcgtc
     1261 cttgacgaac gcctccccgc gggctttgcc cacgcggccg ccgccgcgaa ggatctccac
     1321 ccgcagttcc agctggtccc ccgggacgac gggttttcgg aatttgacct tgtcgatgcc
     1381 cataaagaag gccagtttgt ttttcagttc cggtttgctg agcatgagca cacaggccgc
     1441 ctgggccatg gcttccacga tgaggacgcc cggcatgatg ggctggccgg ggaagtgccc
     1501 ctggaaaaac ggttcgttcc ccgagacgga tttatacccc gtgatctttt tgtccggttc
     1561 gacgatgacc gccttgtcca ccatcaggat cggatagcgg tgagggatga tcttcatgat
     1621 gtccgtggtc cccaacattt tcggggccgg ggtttgcggg gcggctgtgt cttgcattga
     1681 ggaagtcctc cgtgagttga ctgaaatctt taacggttga tggcggcggt ttcgagctgg
     1741 gcggcgagct gtttcaccag gttcacgttg tacccgtgcc ccggccggac ggtggttatt
     1801 ttggccagga gcgggtatcc cagcagggaa aggtcgccga ggaggtcgag cgtcttgtgc
     1861 cgcacgaact cgtccggaaa gcgcagcgct ttttccttgt tgtggatctt gtccatgccg
     1921 accacgacgg cgttgtcgag ggagccgccc cgcgccagcc cctggcgttt gagcgcctcc
     1981 acctcgtagt cgaaacagaa ggtgcgtgcc ggggagatct cccggacgaa ggcgtcggtg
     2041 gaaaaatgaa aaacgtagtt ctggcgctgg atgagagggt gtttgaattc gatggtggtc
     2101 tccaggacca attcgtcggc cggctccacg cggtattcgg tctccccccc tttgtagacg
     2161 aacgggtcgg gacggagata ggtccggtgt ttgttctggt cgcgcacacc ggccgcccgg
     2221 agggtttcga tgaacggacg tgcgctgccg tcggccaccg gaggttcgtt ggcgttcacc
     2281 tcgatggcga ggttgtcgat ccccagcccg gagagggccg ccagcacgtg ttccacggta
     2341 tgcacgcggg cttcgtggtc cttttccccg cccaggctca gggtggtgcc gcggatcacg
     2401 ctggagacga tgcggtagga agcgggcagt cgcggtttgc cggggaggtc ggagcggacg
     2461 aacacgatgc cggaatcttc cggcgcgggc ttgaacacca ggtggcagcg gttgcccgtg
     2521 tgcaggccga cgcctttgta cacggcttct tttccgatgg tgcgttgcgg ttcttgggag
     2581 gggacgagcg gcatgaggtt aaagggacag tttcttcttc aactttttga gttcttcgag
     2641 gagctcggga agttttcgga cggcggccag ggttttcagt tcgtccttca gcggctgggc
     2701 gggcacgccc cagacgacct cgcggggttt gatgtcgtgg gagatgccgc ctttggcccc
     2761 gcagacgacc ccgtcgccga tggagagatg tccggccacg cccacctggg cggcgagggt
     2821 cacgtaattg cccattttca ccgagccggc cacccccgtc aaggccacga ggatgcagtg
     2881 ttcgccgatc tcgacgttgt gggcgatctg gaccaggttg tcgatcttcg tgccgcgtcc
     2941 ga
//
LOCUS       DAOTFD010000284         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_26996_length_2900_cov_4.236907, whole genome shotgun sequence.
ACCESSION   DAOTFD010000284 DAOTFD010000000
VERSION     DAOTFD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26996_length_2900_cov_4.236907"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="P5079_11245"
     CDS             <1..1403
                     /locus_tag="P5079_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30595.1"
                     /translation="TLQGAVTGNSQNITGLGTLGAANINAHTLQGAITGNNQNITGLG
                     TLGATNITAFTLQGDVTGNTYDITGLGTLGATNINAHTLQGAITGNSQNIVGVGNVSL
                     VKMISSNSVAITSATPVAANNVVLVASGTAAAGQQLFQVKGNGDVAVGTATVSDSTLP
                     DLTVTGSSFFGAGGSYKVTNTGQTTLNVINTPKIVGTQPASTGTELDFQVTNNANSSF
                     LWWNGTTNLMRLDNGGQLTTDAGMVPNGSPDLAEFIQADKDVEAYDLVSLAENNGVPM
                     GGQWEDKARVEKTKTPYDARILGVISDGSSALLVNSHMKGLDRKHPGKPLVLAGRVPI
                     KVCLENGPIKKGDYLTSSSKPGYAMKATEPGATVGIALEDFDGASGKTGKVLCFVDIG
                     ERNLTQVVRELKSRSEALERENQALRTRLDGLSDLDALKSQLINLQMRLEHMEQRNRK
                     GGPRLTNMNESSGGKH"
     gene            1525..>2900
                     /locus_tag="P5079_11250"
     CDS             1525..>2900
                     /locus_tag="P5079_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30596.1"
                     /translation="MTDSEKRHPLNKLLLSYKGRLFPTPTFLWFLAGLLVVGVAFGVY
                     ANTLKNGFVWDDHILIADNPFVQDWNNVGEVLSWDYFFRTSPIKTENASRPVWILSAL
                     LDCKLWGLTPAGHHLTNVLLHALNSLLVFVLAAMFLMGKTDPESGGGRTRAPGFGPRP
                     LAFSILAALLFAVHPIHTEPVAAVGFRADLLATLFFLAAFIFYKAFQSALEKTFGNGG
                     THKSPLIAPPALLKAWPWLALSVVAYGLGLLSKEMAVTLPFVLILHDFLLKTGAQWRP
                     LLKKAPAYAGYFLLWPLYAVFHFRRFDYYFGDMAVKWVERLTSLLTFALPVRAESVVS
                     PTPSMSALWAGDPVTNLFTMSTVFVSYLKLLVWPAGLRAEHMVTPLTQPTAAVVLSLA
                     LLGGLAAFAVAVHKKSPAVSFLILAFFVALLPVANLRPLYNVMAERYLYLPSVFFCLL
                     AVYSVSMA"
BASE COUNT          572 a          849 c          879 g          600 t
ORIGIN      
        1 acacgttgca gggggcggtg acgggcaaca gccagaacat cacgggattg gggacattgg
       61 gagcggcgaa catcaacgcc cataccttac agggcgccat caccggcaac aaccagaaca
      121 tcacggggtt ggggacgttg ggagccacca acatcacggc gttcactctg caaggggacg
      181 tgacgggcaa cacctacgat atcacggggc tgggaacgtt gggagccacc aacatcaacg
      241 cccatacgtt gcagggcgcc atcaccggga acagtcagaa cattgttggc gtgggcaatg
      301 tctctctcgt gaaaatgatt agttccaatt ccgttgcaat caccagtgct acgcctgtgg
      361 cggccaacaa cgtggttctt gtggcatctg ggaccgcggc ggcagggcag caactgttcc
      421 aggtcaaagg gaacggggac gtggcggtcg gaacggcgac ggtttccgat tccactctcc
      481 cggacctgac cgtgaccggt tccagcttct tcggcgccgg cgggagttat aaggtgacga
      541 atacgggaca gaccacattg aacgtcatca atactccgaa gatcgtgggg actcagccag
      601 ccagcacggg aacggaattg gatttccaag tgacgaataa cgccaattcc agtttcctgt
      661 ggtggaacgg gacgacaaac ctcatgcggc tggataacgg cggtcagttg accacggatg
      721 ccggcatggt tcccaacggg agccctgact tggccgagtt catccaggcg gataaggacg
      781 tcgaagccta tgatctcgtc tcgttggcgg aaaacaacgg agttcccatg ggcgggcaat
      841 gggaagataa ggcccgggtg gaaaaaacga agacgcccta tgacgcgcga atcctcggag
      901 ttatctcgga cggatcgagc gctctcctgg tcaactctca catgaagggg ttggaccgga
      961 agcatcccgg caagccgctt gtcttggcgg gtcgtgttcc catcaaggtc tgcttggaaa
     1021 atggaccaat aaagaagggg gactacctga cctcctcctc caagcccggg tatgccatga
     1081 aggccacgga acccggcgcc acggtgggca tcgccttgga ggacttcgac ggcgcctccg
     1141 ggaaaacagg caaggtcctt tgtttcgtgg acatcggcga aaggaacctc acccaggtcg
     1201 tgcgggaact gaagtcgcgc agcgaggcgc tggagcggga aaatcaggcg cttcgcacgc
     1261 gtctggacgg cctcagcgac ttggacgccc tcaagagcca gttgatcaac ctgcagatgc
     1321 ggctggaaca catggaacaa cgcaaccgca aaggcgggcc gcgtctcacc aacatgaacg
     1381 aatcgtcggg agggaaacac tagaagataa ggatcggctg ggaggaacct atggcgaccc
     1441 cacgtcagcg cctttgggat ccgcggcggc ccatcgggcc gacaactccg ctggcaagga
     1501 gaccatggaa aggagggatg gaaagtgacc gacagtgaga agcggcaccc actgaataag
     1561 ttactacttt cctataaagg gcggctgttc ccgacgccga ccttcttgtg gttccttgcc
     1621 ggcctgttgg tggtgggagt ggccttcggc gtgtacgcca ataccctgaa gaacggtttc
     1681 gtgtgggacg accatatcct catcgccgac aaccccttcg ttcaggattg gaacaacgtg
     1741 ggggaagtcc tttcctggga ctattttttc cggacctcgc cgataaagac ggaaaacgct
     1801 tcgcgccctg tctggatcct ctcggccctg ctcgattgta aattgtgggg gctcaccccg
     1861 gccggccatc acctgaccaa cgtccttctc catgcgctga acagcctgct ggtctttgtc
     1921 ctggcggcga tgtttctgat gggaaaaacg gatccggagt ccggcggagg caggactcgg
     1981 gctcccggat tcggtccccg gcccctggcg ttttccattc tggccgcgct cttgttcgcc
     2041 gtgcatccca tccacaccga acccgtggcg gccgtggggt ttcgcgccga cctcctggcc
     2101 acgctgtttt tcctggccgc gtttattttc tataaggcgt ttcaatccgc tctcgaaaaa
     2161 acatttggga atggcggcac ccataaatcc ccgcttatcg cgccgccggc gctgttgaaa
     2221 gcgtggccgt ggctggcgct ttccgtcgtc gcctacggct tgggcctgct gtccaaggag
     2281 atggctgtca cgttgccgtt cgttttgatc ctgcatgact ttttgttgaa aaccggcgcc
     2341 cagtggcggc cgctgttgaa gaaagctccg gcgtatgccg ggtacttcct tttgtggccg
     2401 ctctatgcgg tgttccattt ccgccggttc gactattact tcggggacat ggccgtgaaa
     2461 tgggtcgaac ggctcaccag cctcctcacg ttcgccctgc cggtccgcgc ggaaagcgtg
     2521 gtgagcccca ccccgtcgat gtcggccctg tgggccgggg acccggtgac gaacctgttc
     2581 accatgtcga cggtgttcgt ctcctacttg aaactcctcg tttggccggc gggactgagg
     2641 gcggaacaca tggtcacacc gcttacgcaa ccgacggcgg cggtcgtcct ctcgttggct
     2701 ctcctgggcg gcctggcggc ttttgccgtt gccgtgcata agaaatcgcc ggccgtcagt
     2761 ttcctgatac ttgctttctt cgtcgcgctt ctgcccgtcg ccaaccttcg gccgctctac
     2821 aacgtgatgg cggaacggta tctctatctg ccttctgttt tcttctgctt gctggcggtc
     2881 tacagcgtct cgatggcgtt
//
LOCUS       DAOTFD010000285         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_27113_length_2892_cov_3.556574, whole genome shotgun sequence.
ACCESSION   DAOTFD010000285 DAOTFD010000000
VERSION     DAOTFD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27113_length_2892_cov_3.556574"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="P5079_11255"
     CDS             <1..2111
                     /locus_tag="P5079_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY30597.1"
                     /translation="RASLEIMSQKKPFRDLLNKAETKDGRVLWLSTNGIPLLDADGNL
                     RGYRGSDSDVTERKKAEEALRASEAQLNNALMMATLGPWEYDVPTDKFTFTDAFYAIF
                     HTTAEQVGGYVVSSADYAKRFVHPEDMSIVSREVRGAIETNDPNYSRRFEHRIVYANG
                     GVGYISIRFFIVKDAQGRTVKTYGVNQDITERKKAEETLRHEKERLAVTLRSIGDAVV
                     TTDVQGRVTLMNKVAEGLTGWKEADAAGRALEEVFRMADEEPGQRREGLVSEMLRAGD
                     GKEPVVRATLVSRDGTRRVIAETVAPIRDKDGQAVGLVLVFRDMTERQKMEDELLKVS
                     KLDALGMLAGGLAHDFNNILTGILGNINLAKLVAPPMSEMVEMLSEAENASLEAKNLT
                     HQLLTFARGGRPVKKIIDPGKIVRETAFFVARGSKVKCEFRIAADLHPVEADEGQITQ
                     VVSNIVINAVQAMPQGGTLEVSAENLAVSGGTLPLPAGPYVKLSLKDFGVGISAEHLP
                     KIFDPYFSTKQFGSGLGLATSFSIVKKHGGHLTADSRLGEGTTFHIYLPASPGKTVAR
                     PPSERKVIRGSGRILVMDDDRFVRSLAMRLFPDLGYEADFAEDGGQAVEMFRKAREGG
                     KPYAAVVLDLTVPGGMGGKEALSALREIDPAVKAVASSGYSNDPVMANHKEYGFAEIL
                     MKPYRMVDVAEVLHRLLTGA"
     gene            2246..2563
                     /locus_tag="P5079_11260"
     CDS             2246..2563
                     /locus_tag="P5079_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020199041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRY30598.1"
                     /translation="MSVIDQDFQTNRKKVGDHKGHAVWGPEEKPAKLGIHGTWVAVDF
                     DLCTGDGACLDACPESVFEWVESPGHASSAKKADPAKEEACIFCMACESVCPATAIKI
                     TPK"
     gene            2589..>2892
                     /locus_tag="P5079_11265"
     CDS             2589..>2892
                     /locus_tag="P5079_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30599.1"
                     /translation="MDAPPLSKKMRMKDILAAFPSAQRTLYQKFHVGGCMSCCYQETD
                     TLEELCHRRGIDVEEALRQMNTRADERVIETFSRQPIYYKCNRFAVVGPDHDIVWPS"
BASE COUNT          609 a          828 c          956 g          499 t
ORIGIN      
        1 cgcgcgcgtc tctcgagatc atgtcccaga aaaagccttt ccgggacctc ctcaataagg
       61 cggagaccaa agacggccgg gtcctctggc tgtccaccaa cggcatcccc ctgttggacg
      121 cggacgggaa tctccgggga taccgcggct cggattcgga cgtgaccgag cggaaaaaag
      181 cggaggaggc gctgcgggcg agtgaggcgc agttgaacaa cgccctgatg atggcgaccc
      241 tcggtccatg ggaatatgac gtgccgacgg ataagttcac gttcaccgac gcgttttacg
      301 ccattttcca tacgaccgcc gagcaggtgg ggggatatgt cgtctcttcc gccgattacg
      361 cgaaacgttt tgtccacccc gaggacatgt cgatcgtcag ccgggaggtc cggggagcga
      421 tcgaaacgaa tgatccgaat tacagccgcc ggttcgaaca ccgcatcgtc tatgcgaacg
      481 gcggggtcgg atacatcagc atccggtttt ttatcgtgaa ggacgcccag ggaaggaccg
      541 tcaagacata cggcgtgaac caggacatca ccgagcggaa aaaagccgag gagacgctgc
      601 ggcacgagaa ggaacggctg gcggtcacct tgaggagcat cggcgatgcg gtcgtcacga
      661 cggacgtgca aggacgggtg accctgatga acaaggtggc cgaggggttg acgggctgga
      721 aagaagccga cgccgccggc cgcgcgttgg aagaggtgtt tcgcatggcc gacgaggagc
      781 ccggtcaacg ccgcgagggg ctcgtcagcg agatgctccg cgcgggagac ggcaaggagc
      841 cggtcgtccg cgcgacgctg gtttcccggg atgggacccg gcgggtgatc gcagagaccg
      901 tcgcgcccat ccgcgacaaa gacggtcagg ccgtcgggct cgtcctggtt ttccgcgaca
      961 tgacggaacg gcagaaaatg gaggacgaac tgctcaaggt cagcaaactg gacgcgctgg
     1021 ggatgttggc gggcggcctt gcgcacgatt tcaacaacat cctcacgggg atcttgggca
     1081 acatcaacct ggcgaaactg gtcgcgccgc cgatgagcga gatggtcgaa atgctgagcg
     1141 aggcggaaaa cgcttcgctg gaagcgaaga atttgacgca tcagctcctc acgttcgccc
     1201 ggggcggaag gcccgtgaaa aagatcatcg acccgggaaa gatcgtgagg gagacggcgt
     1261 tcttcgtggc ccggggctcc aaggtgaaat gcgagtttcg tatcgccgcc gacctccatc
     1321 cggtggaggc cgacgaagga cagatcacgc aggtggtcag caatatcgtg atcaatgccg
     1381 tgcaggccat gccccagggg gggactttgg aggtgtcggc ggagaacctg gctgtttcgg
     1441 gcggaaccct gccgcttccc gccggcccgt acgtcaaatt gtccctcaag gacttcggcg
     1501 tcggcatctc cgccgaacat ctgcccaaga ttttcgaccc ctatttttcc acaaaacagt
     1561 tcgggagcgg attgggtttg gccacaagtt tttccatcgt caagaaacac ggcggtcatc
     1621 tgacggcgga ctcccggttg ggggagggga cgacgttcca catctatctc cccgcctctc
     1681 cggggaaaac cgtggcccgc cccccgtcgg aacggaaggt cattcgggga agcgggcgga
     1741 ttctggtgat ggacgacgac cggttcgtcc ggtcgctggc gatgcggctg ttcccggatt
     1801 tggggtatga ggcggatttc gccgaagacg gcggacaggc cgtggagatg ttccggaaag
     1861 cccgggaggg ggggaaaccc tacgccgcgg tggtgttgga cctcaccgtg cccggcggca
     1921 tgggaggcaa ggaggcgctg tcggctctgc gggagatcga cccggccgtg aaggccgtgg
     1981 cctccagcgg ctactcgaac gatcccgtca tggcgaacca caaggaatac gggtttgccg
     2041 agatcctcat gaagccctac cggatggtcg atgtggcgga ggtgttgcac cggctgttga
     2101 cgggggcttg aggttccgtt gcccccgaac ggcgcgtggt tcgtccgggc cggcggcttt
     2161 actttaattt ccccgaatgg taaaattcat atgttgggtg ccgcagaaaa attccacttc
     2221 acgacatcgc acaaaggaga attttatgtc cgtcattgac caggattttc agaccaatcg
     2281 aaagaaagta ggcgaccaca agggccacgc cgtgtggggg ccggaagaaa aaccggccaa
     2341 gctcggcatc cacggcacgt gggtggcggt ggacttcgat ctgtgcaccg gcgacggcgc
     2401 ctgcctcgat gcctgtccgg agagcgtgtt cgagtgggtg gaatcccccg gccacgcctc
     2461 ctccgccaaa aaggcggacc cggccaaaga ggaagcctgt atcttctgca tggcctgcga
     2521 aagcgtctgc cccgccaccg ccataaaaat cacaccgaag taagccgcgc gagaaaagga
     2581 acccgccgat ggacgccccg cctctttcca agaagatgcg gatgaaggac attttggcgg
     2641 cgttccccag cgcccagcgg accctctatc agaaattcca cgtcggcggc tgcatgagct
     2701 gctgttatca ggagacggac acgctggaag aattgtgcca ccgccggggg atcgacgtgg
     2761 aagaggccct gcggcagatg aacacgcgcg ctgacgagcg tgtcatcgag accttcagcc
     2821 gccagcccat ctattacaaa tgcaaccgct tcgcggtagt tggtcccgat cacgacattg
     2881 tgtggccaag ct
//
LOCUS       DAOTFD010000286         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_27211_length_2885_cov_4.091873, whole genome shotgun sequence.
ACCESSION   DAOTFD010000286 DAOTFD010000000
VERSION     DAOTFD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27211_length_2885_cov_4.091873"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /gene="rpsM"
                     /locus_tag="P5079_11270"
     CDS             complement(<1..313)
                     /gene="rpsM"
                     /locus_tag="P5079_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY30600.1"
                     /translation="MARIAGVDLPKHKKIEIALGYIYGIGRFTAGNICREVGVNPTTR
                     VKDLTESEASKLNMEISKNYKVEGDLRREVQQNIRHLIDIGTYRGLRQRRNLPVRGQR
                     TK"
     gene            complement(402..515)
                     /gene="rpmJ"
                     /locus_tag="P5079_11275"
     CDS             complement(402..515)
                     /gene="rpmJ"
                     /locus_tag="P5079_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRY30601.1"
                     /translation="MRVRASVKPICKKCRVVRRRRKIYIVCDNPRHKQRQG"
     gene            complement(512..733)
                     /gene="infA"
                     /locus_tag="P5079_11280"
     CDS             complement(512..733)
                     /gene="infA"
                     /locus_tag="P5079_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRY30602.1"
                     /translation="MVKEEKIEVEGTVLEALPNAMFRLEIEGGHRVLAHISGKMRMHY
                     IKILPGDKVRVELSPYDLTRGRIIYREKV"
     gene            complement(759..1529)
                     /gene="map"
                     /locus_tag="P5079_11285"
     CDS             complement(759..1529)
                     /gene="map"
                     /locus_tag="P5079_11285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY30603.1"
                     /translation="MSSEARRIELKSEKDLQHMRHAGRLAGQVLEKVGEAVRPGIKTK
                     ELDRLAEKFIRAAGGIPTFLGYRGYPACLCVSINEEVVHGIPDASRMLREGDIVSVDV
                     GVTLEGFVGDTAATFAVGKVPAESQKLLDVTRECLRKGIEQARVGGRLGDVSHAIQEH
                     AESRGYGVVRDYVGHGIGRQMHEEPVVPNYGSPRTGVRLEAGMTLALEPMITLGHWKV
                     RVLDNGWTVVTQDGKRAGHFEHTVAITSAGPEILTLPH"
     gene            complement(1526..2179)
                     /locus_tag="P5079_11290"
     CDS             complement(1526..2179)
                     /locus_tag="P5079_11290"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRY30604.1"
                     /translation="MNVVLLGAPGSGKGTQAERLQKRYGIPHVSTGAIFRDEISKKSD
                     VGLRVQEYVKSGGLVPDELTVEIISRRLAQSDCAAGFLLDGFPRTVAQAEALDGFLSK
                     GKKEMDAVVSLELSEAAAVKRLSSRRQCPKCGKIYNLAFQPPKQEGLCDADGGALGQR
                     EDDKPETIRKRLMVFNDLTQPLIAYYRGEGILSTVNGDEGVDAVSRAIYEVLDRFKK"
     gene            complement(2199..>2885)
                     /locus_tag="P5079_11295"
     CDS             complement(2199..>2885)
                     /locus_tag="P5079_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HRY30605.1"
                     /translation="RLRRGVATALVVVTGLVVWVETGQRKIPVQYAKRVVGRKMYGGA
                     STFLPLKVDQSGVIAVIFAVSLLSVPVTIASFAPNAGWASKISQWWNGGVWWYEFFYA
                     GLIIFFCYFYNSVQFNPTDLAENLKKWGGFIPGIRPGENTANYIQKVLERITLGGALF
                     VAALAVLPDYLRRSLNAPFFFGGTSILIVVGVALDTMGQLESHLIMRHYEGFMKHGRI
                     KGRWFNVGAS"
BASE COUNT          566 a          978 c          759 g          582 t
ORIGIN      
        1 acttggtgcg ctgtccgcgc accggcaggt tgcgccgctg acgcaggccc cgataggtgc
       61 cgatatcgat caggtggcgg atattctgct gcacttcgcg gcgcaaatcg ccctccacct
      121 tgtaattctt tgaaatctcc atgttgagct tggacgcctc cgactccgtc aggtctttca
      181 cccgggtcgt cggattgacg cccacctccc ggcaaatgtt gccggcggtg aagcgtccga
      241 tcccatagat atatcccagc gcaatctcga tcttcttatg tttcggcaaa tccacgccag
      301 cgattcgtgc catgatttat cgcacctcca aagttataaa aatgccggga gccggggcgg
      361 agaaccggag acttcccggt cgttccgtcg acccgcccga cctatccctg ccgctgtttg
      421 tggcgggggt tgtcacacac gatataaatc ttcctccgcc gacggacgac gcggcatttt
      481 ttacagatcg gcttcaccga tgcccgtacc ctcatacctt ttccctgtag atgatccggc
      541 cgcgcgtcag gtcgtacggc gacaactcca cccgcacctt atcgcccggc agtatcttga
      601 tgtagtgcat gcgcatcttc ccggaaatgt gcgccaacac ccgatgcccg ccctcgattt
      661 ccaggcggaa catggcgttg ggcaacgctt ccaaaaccgt cccttcaacc tctatctttt
      721 cttctttcac cataaatttt cccgttatcg aaatatcgtc aatggggcag ggtcaatatc
      781 tccggcccgg ccgaggtgat ggccaccgta tgctcgaaat gccccgccct cttcccgtcc
      841 tgcgtcacca cggtccatcc gttatccaga acccgaacct tccaatgtcc gagggtgatc
      901 atgggttcca gcgccagggt catccccgct tccaaccgaa cgccggtgcg gggcgaaccg
      961 taattgggga ccacgggctc ttcgtgcatc tggcggccga tgccgtggcc gacgtaatcc
     1021 cgcaccacgc cataaccgcg ggactcggcg tgctcctgga tggcatggga gacatctccc
     1081 aaccggcccc ccacgcgcgc ctgctcgatc cctttccgca aacactcgcg cgtcacgtcc
     1141 aaaagtttct ggctttccgc cgggaccttc ccgacggcaa acgtggcggc ggtgtcgccc
     1201 acgaaccctt ccagggtcac gcccacatcg acgctgacga tgtccccttc gcgcaacatc
     1261 cgcgaggcgt ccgggatgcc gtgcaccacc tcctcgttga tggacacaca caggcacgcc
     1321 gggtatcccc gatagccaag gaacgtcggg atgccgccgg ccgcacggat gaatttttcg
     1381 gccaaccggt cgagctcctt ggtcttgatc ccgggacgaa cagcttctcc cactttttca
     1441 aggacctgtc cggccaaacg gccggcatga cgcatgtgct gcaaatcttt ttccgacttt
     1501 aactcgatcc gccgggcttc cgaactcatt ttttgaaacg atccagcacc tcgtagatcg
     1561 ccctggaaac ggcgtccacc ccttcatcgc cgttaacggt ggataaaatc ccctcgccgc
     1621 gataatacgc gatcaggggc tgcgtcaaat cgttaaagac catcaggcgt ttccgaatgg
     1681 tctccggctt gtcgtcctcc cgctggccca gggccccgcc gtcggcgtcg cacagcccct
     1741 cctgcttcgg aggctggaac gccaaattgt atatcttgcc gcacttcggg cattgccgcc
     1801 ggctggaaag acgcttcacc gccgccgcct cggataactc caaactcacc acggcgtcca
     1861 tttctttctt gcccttcgac aaaaaaccgt cgagcgcctc ggcctgcgcc acggtgcgcg
     1921 ggaaaccgtc caaaaggaac cccgccgcgc aatcggattg cgccagacgg cgggaaatga
     1981 tctccaccgt cagctcgtcc ggcaccaggc cgccggattt cacgtattcc tggacgcgca
     2041 gcccgacgtc ggattttttg gaaatctcat ccctgaagat ggcgccggta gacacatggg
     2101 ggatgccgta ccttttctga agacgttcgg cctgtgttcc cttgccggac ccgggcgctc
     2161 ccaataaaac gacgttcatg acggccttct ttccgagatc aggatgcgcc gacgttgaac
     2221 cagcgtcctt tgatgcgtcc gtgcttcata aagccttcgt agtgccgcat gatgagatgg
     2281 gactccaact ggcccatcgt atcgagcgcc acgccgacga cgatcaaaat cgaagtgccc
     2341 ccgaaaaaga agggagcgtt caaactgcgg cggaggtagt ccggcaacac cgccagggcc
     2401 gccacgaaga gcgcgccgcc cagcgtgatg cgttccagga ctttttgaat gtaattggcg
     2461 gtgttctccc ccggccggat gcccgggatg aacccgcccc acttcttgag attttcagcc
     2521 aagtccgtcg gattgaactg caccgagtta taaaaatagc agaaaaagat gatcagcccc
     2581 gcgtagaaaa attcatacca ccataccccg ccgttccacc actgggatat cttcgaggcc
     2641 caccccgcgt tgggggcgaa cgaggcgatg gtcaccggaa cggacagaag ggagaccgcg
     2701 aaaatgacgg cgatcacgcc ggactggtcc accttcaacg gcaggaacgt gctcgcgccg
     2761 ccgtacatct tccggccgac gacccgcttg gcgtattgca ccgggatctt cctttggccc
     2821 gtctccaccc acaccaccag gccggtcacc accaccagcg cggtggccac cccgcggcgc
     2881 aggcg
//
LOCUS       DAOTFD010000287         2867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_27490_length_2867_cov_3.528450, whole genome shotgun sequence.
ACCESSION   DAOTFD010000287 DAOTFD010000000
VERSION     DAOTFD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27490_length_2867_cov_3.528450"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2660)
                     /locus_tag="P5079_11300"
     CDS             complement(<1..2660)
                     /locus_tag="P5079_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY30606.1"
                     /translation="MCLACWSGLASAVKLTWGNVTQLAGKPGWFITARNGNTSSIRPG
                     LDVRGADTLSFMVKRDALSGAADFLVGVKDWGPNGSLNDGDEIEKKVSIGPYISGGVV
                     DTEYRQVQIPLSVLTNPQVHTEKLETINIEPLTAAATIYVDNFVFLDSVSPSAPTNMQ
                     DDGGVVANDHVFNATNTLTVQAAAGSTDKTLESVRVEYSTDSGTTWSTIGRDYDVTDD
                     TYTFTWYTTALSTSATYYVRAVSEDASGNMGLMAAFTDCDVDWKTAAPGSFAAAAQSS
                     TSVRFDWLLALGATAYQVQTATAGLIAELPDPTSYYIQTDLTANTSSQITRVQSFNAG
                     GGGGFTVLADSPVFTLANVPLGVEVAGVYGSSVALNWTANDNRVGTRFEVSQSTDEFA
                     LDFSTPVPLASNYAVYSATVTGLLPGTTYWFRLRAFNDDDIASVFSSTVSTSTLGGPA
                     APGNFRATASSLTVRWDWDLSTGATAYFLLSSTNGILAALNDPTSYYVQTKDILGNDL
                     QPNTSSYITNIQASNGYGVSDYSYVTSSPVYTLANAPNNLTVMGVHYTTVTLQWTPSG
                     NQSGTPYEISMSTVDSGFTSGVSTPVPFAQNLTGMTTTLASLSPGATYYFRVRAKNGD
                     DVVSEGFSPVASTTTLSFGVVINEIAWGGTAAGSSDEWVELYNSGSGSVDLAGCSLVI
                     QATTIPLSGVLGAGQYYLLERTDDSTVSDIAADWFGSFGGSGIPNTGGALTLYISSTN
                     GIVADSVSFAGSWPAGSATPNYYTMERVSATVSGSQSSNWASNNGVTRNGLDAGSNPL
                     NGTPKALNSTTPDSWPPSAISDLTALSGANDGEISLSWTSPGDNGVTGDISGGQFVIT
                     YTTVGIINDFNAPPTPVTMRAITPLSGSRA"
BASE COUNT          526 a          889 c          935 g          517 t
ORIGIN      
        1 ttcgccctcg atccgctgag cggcgtgatg gccctcatcg tcaccggcgt gggcggggcg
       61 ttgaaatcgt tgatgatgcc cacggtggtg tatgtgatga cgaactgacc gccggaaatg
      121 tcgccggtca cgccgttgtc gcccggcgac gtccacgaca gggagatttc cccgtcgttc
      181 gcgccggaaa gggcggtgag gtccgaaatg gccgacggcg gccaggagtc gggggtggtg
      241 ctgttcagcg ctttgggcgt gccgttgagc gggttgctgc cggcgtcgag tccgttgcgg
      301 gtcacgccgt tgttgctggc ccagttggag gactgagacc cggagaccgt ggcgctgacc
      361 cgctccatgg tgtagtaatt cggggtggcg ctgccggccg gccaactgcc ggcgaacgaa
      421 acggaatcgg cgacgatccc gttggtggag gagatataca gggtcaaagc gccgcccgtg
      481 ttggggattc cggagccgcc gaaactcccg aaccaatccg ccgcgatgtc gctgaccgtg
      541 ctgtcgtcgg tcctctccaa aagataatac tgtccggcgc caaggacgcc cgacaatgga
      601 atggtcgttg cctggatgac cagcgaacaa cccgccaaat cgacgctgcc gcttccggaa
      661 ttgtacagtt cgacccattc gtcggacgaa ccggcggcgg tgccgcccca ggcgatttcg
      721 ttgatgacca cgccgaagga cagcgtggtt gtgctggcca cgggagagaa cccttccgaa
      781 acgacgtcgt ccccgttctt cgcccgcacg cggaaatagt aggtggcgcc gggcgacagg
      841 gaagcgagcg tggtggtcat ccccgtcagg ttctgagcga acggcaccgg ggtggacacg
      901 ccgctggtga aaccggaatc caccgtgctc atcgaaatct catacggcgt ccccgactgg
      961 ttcccgctcg gggtccactg gagggtcacg gtggtgtaat ggacgcccat gaccgtcagg
     1021 ttattgggcg cgttggccaa cgtatagacc ggagaagagg tcacgtaaga ataatcgctg
     1081 acgccgtaac cgttggacgc ctgaatgttg gtgatatagg aagaggtgtt gggctgcagg
     1141 tcgttgccca aaatgtcctt ggtttggacg taatagctgg tggggtcgtt caaggccgcc
     1201 agaatgccgt tggtggaact caggaggaaa tacgccgtgg cgccggtcga caggtcccag
     1261 tcccagcgga cggtcagaga ggatgcggtg gcccggaaat tcccgggggc cgctgggccg
     1321 ccgagggtgc tggtgctgac ggtggaggag aacacggagg cgatgtcgtc gtcgttgaag
     1381 gcgcgcaggc ggaaccagta ggtggtgccc ggcaaaagac cggtgacggt ggcggaataa
     1441 accgcgtagt tgctcgccag cgggaccggc gtggagaaat ccagggcgaa ctcgtcggtg
     1501 gactgcgaca cctcgaaccg cgtgccgacc cggttgtcgt tggccgtcca gttcaaagcc
     1561 acgctggaac cgtagacccc ggcgacctca acgcccagcg gcacgttggc cagggtgaac
     1621 accggcgaat cggccagcac ggtgaatccc cctccgccgc ccgcgttgaa cgactgcacc
     1681 cgcgtgatct gcgacgaggt gttggccgtc agatccgtct gaatgtaata gctggtggga
     1741 tccgggagtt ccgcgatgag tcccgccgtc gccgtctgca cctgataggc cgtggcgccg
     1801 agcgccagga gccagtcgaa gcggacggac gtgctggact gcgccgccgc ggcaaaagaa
     1861 cccggggccg ccgtcttcca gtccacgtcg caatccgtga acgccgccat taaacccatg
     1921 ttgccgctgg cgtcctcgga cacggcgcgc acgtagtagg tggccgaggt gctcagcgcg
     1981 gtggtatacc acgtgaaggt gtaggtatcg tccgtcacgt cgtagtcccg gccgatggtc
     2041 gaccacgtgg tgccgctgtc cgtggaatac tccacccgca cgctctccag cgtcttgtcg
     2101 gtcgacccgg cggcggcctg cacggtgagg gtgttggtgg cgttaaaaac gtggtcgttg
     2161 gccaccacgc cgccgtcgtc ctgcatgttc gtcggtgcgg acgggctcac gctgtccaga
     2221 aacacaaaat tgtccacgta gatcgtcgcc gccgcggtca acggctcgat gttgatggtt
     2281 tccaatttct ccgtatgcac ctgcggattc gtcaaaaccg acaacgggat ctggacctgc
     2341 ctatactccg tatccaccac gccgcccgaa atatacggcc cgatggacac cttcttttca
     2401 atctcgtccc cgtcgttcag ggacccattc gggccccagt cttttactcc caccaaaaag
     2461 tccgccgcgc cggaaagagc gtcccgtttc accatgaaac tcagcgtgtc ggccccgcgc
     2521 acgtccaacc cgggccgaat gctcgacgtg ttcccgttcc gcgccgttat gaaccacccc
     2581 ggtttccccg ccagttgcgt cacatttccc caggtcaact tcaccgccga cgccaagccc
     2641 gaccagcagg ccaagcacat agcggtgcca tgtcttcatg atccgtcccc tctccccgtc
     2701 atttcggtga agctaacgca ctcaccgcga cgggcaagag gaaatccggc gcctacttcg
     2761 cccgcagcgc cgcctgagcc gccgccagcc gggcaatcgg cacgcggtag ggcgaggccg
     2821 agacgtaatc gaggccggtc ttttcacaga aggcgatcga gcgcgga
//
LOCUS       DAOTFD010000288         2855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_27686_length_2855_cov_5.372500, whole genome shotgun sequence.
ACCESSION   DAOTFD010000288 DAOTFD010000000
VERSION     DAOTFD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27686_length_2855_cov_5.372500"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="P5079_11305"
     CDS             <1..676
                     /locus_tag="P5079_11305"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY30607.1"
                     /translation="IAHARSETLLLNILPASIAKRLKEHPDATIADDYPSATVLFADL
                     VGFTEMCTLLSPNETVDLLNRIYSHFDTLAEKYGLEKIKTIGDAYMVVGGVPVETDRH
                     ARSVGLFALRLQEIAKSFSFEQGAISLRIGIHTGRLVAGVIGKKKFSYDVWGDTVNIA
                     NRMESHGVPGKIHCSEAFHAQARADFDFTERGEVDIKGKGKMRTYFLEKQKPSAPPPG
                     SSAKAG"
     gene            684..1331
                     /locus_tag="P5079_11310"
     CDS             684..1331
                     /locus_tag="P5079_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HRY30608.1"
                     /translation="MGRYGRYVGKVYAKFLPNGRDMQLLKPFKYIDPHKKVWPAPARS
                     IVDGASIPQFAWSIIGGPFEGKYREASVIHDVACDKKNRPWAAVHEMFYYAMRASGVG
                     SVKAKIMYAAVHYFGPRWYTKAELNAALRRMLVGPVGRRPLLETGARMRSPKYKLAKY
                     PLAGAPLAPPPPPPAPPGLTPANFKELQQFITDREKATKNQLSLAEIRDFRPAEQ"
     gene            1616..2785
                     /locus_tag="P5079_11315"
     CDS             1616..2785
                     /locus_tag="P5079_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30609.1"
                     /translation="MKISRFFTCLLLIIFCTVPNIYPADDIQSQFENSWNFYSNRVKQ
                     GASEAERLSILDRIEKKYEGQGIDLAFVKTEKGKLLGAKEKEAAEIAQRKDEFYYEWG
                     KWKKETAPHWSAEVQYGQLNYFDRKYGDIPEVKEKIQLEKERLQGPTNTPMQAPAPPI
                     QSMETSAPQDNRLQLSPKVFGQIDYWLKDSAFEEEEKALKDVTGELIVLGYDASSANY
                     SISNGLGIRGGLLFPVGDGTFHIGGSVGYIWGPTWESETNGYSWSYGNGRSEREDTVN
                     YLRFLLEAKKAIPLNNDVDFVFRGGLGLASGRMETTITNSGTFVTALGASTSSSQSET
                     WSGFTWEFLPSLLIKGNGVNIEFGVGFAQFPTLKETEDFYEFEWAPIGFRAAVEF"
BASE COUNT          698 a          864 c          726 g          567 t
ORIGIN      
        1 catcgcccat gcccggtcgg aaacgctcct gctcaacatc ctccccgcgt cgatcgccaa
       61 acgcctcaag gaacacccgg acgccaccat cgccgacgac tacccctccg ccacggtcct
      121 gttcgcggac ctcgtggggt tcacggagat gtgcacgctg ctctcgccga acgaaaccgt
      181 ggacctcctc aaccgcatct actcccactt cgacaccctg gcggaaaaat acgggctgga
      241 aaagataaaa accatcggcg acgcctacat ggtggtgggc ggcgtcccgg tggaaaccga
      301 ccggcacgcc cggtccgtgg ggcttttcgc cctgcgtctg caggagatcg ccaaatcctt
      361 ctccttcgaa cagggcgcca tctccctgcg catcggcatc cacacgggac gcctcgtcgc
      421 cggggtcatc ggcaagaaaa agttctccta cgacgtctgg ggggacaccg tcaacatcgc
      481 caaccgcatg gaatcgcacg gggtgcccgg caaaatccac tgttcggaag cgttccacgc
      541 ccaggcccgg gcggatttcg atttcaccga acggggcgag gtcgacatca aggggaaagg
      601 caaaatgcgg acctacttcc tcgaaaaaca aaaaccctcg gcgccgccgc ccggctcttc
      661 cgcgaaagcc ggataaaccg cccatgggcc gctacggccg atacgtcggc aaagtctatg
      721 cgaaattcct tcccaacggc cgggacatgc agctgctcaa gcccttcaaa tacatcgatc
      781 cacacaaaaa agtctggccg gccccggcgc ggtccatcgt ggacggcgcc tccatccccc
      841 aattcgcctg gtccatcatc ggcggcccct tcgaaggcaa ataccgggag gcctcggtca
      901 tccacgacgt ggcctgtgac aaaaagaacc gcccgtgggc cgcggtccac gaaatgtttt
      961 actacgccat gcgggcgtcc ggagtcggtt ccgtcaaagc caagatcatg tatgccgccg
     1021 tccattattt cggcccgcgc tggtacacca aggccgaact caacgccgcc ctgcggcgga
     1081 tgctcgtcgg cccggtgggc cgtcgccctc tcctggaaac aggcgcccga atgcgctccc
     1141 caaaatacaa actagccaaa taccccctgg ccggcgcgcc cctggcgccg ccccctcctc
     1201 caccggcccc tcccgggctc acgccggcga actttaaaga acttcagcag ttcatcacgg
     1261 accgagaaaa agccacaaaa aaccagttgt ccctcgccga aatccgcgat ttccgccccg
     1321 ccgagcaata gccccttccc ccgtctcctt cgagcaagtc gctttttgac gcgtcgagaa
     1381 gccactcgac cgtcgccgcc ccatttcccc ttgccgccag gcatgacact tgcttgcgga
     1441 aaagtgtagc tgcccaattc attgggcgcc tcaaaaaccg cccctctcct gaactttttt
     1501 caatcctgtt attcctatgt tgactgattt gctaaaattt ccaactgagg gctgccagtc
     1561 cgcgtggatg gatttatctc tttaaccatc tcatcacccg aaaagagacg actgcatgaa
     1621 aatatcgcga ttcttcacat gtcttttgtt aattattttc tgcacagtcc ccaatatata
     1681 tcctgccgat gacatccagt cgcaattcga gaattcttgg aatttttaca gcaaccgggt
     1741 caaacaaggc gccagcgagg cggaaagact atcaatcctc gatcgcattg agaaaaaata
     1801 cgaaggccag ggaattgatt tagcttttgt aaagacggaa aaaggtaagt tactcggggc
     1861 aaaagaaaaa gaggccgctg aaattgcgca acgaaaagat gaattttatt acgaatgggg
     1921 caaatggaaa aaggaaacag ccccccactg gagcgccgag gttcaatatg gccagcttaa
     1981 ctatttcgac aggaaatacg gcgacatccc cgaagtgaaa gaaaaaattc agttggaaaa
     2041 agaacggctt caggggccga caaacacgcc catgcaggcg ccggctcccc ccatccagtc
     2101 aatggaaaca tccgccccgc aagacaatcg ccttcaacta tccccaaaag tgttcggcca
     2161 aatcgactac tggctaaaag attccgcgtt tgaggaagaa gaaaaagccc tgaaagatgt
     2221 caccggcgaa ttaattgttc tcggctacga cgcttcttcc gcaaactaca gcatttccaa
     2281 tggactcgga atccgagggg gcctgttatt ccccgtcgga gacggcacgt tccatattgg
     2341 cggcagtgtt ggatacatct ggggccccac ctgggaatct gaaacgaatg gttacagttg
     2401 gagttatggg aatggccggt cggaacgcga ggacaccgtg aattatttac gttttttgct
     2461 tgaggcaaaa aaagcaatcc ccctgaacaa tgatgtggat tttgtttttc gtggaggact
     2521 ggggctcgcc agcggccgaa tggaaacgac gataaccaat tcaggaacgt ttgtaacggc
     2581 gttgggggca tcgacctcgt cctcgcaaag cgaaacgtgg tccggtttca catgggaatt
     2641 tttgccgtcc cttctaatta aggggaacgg tgtaaatatc gaatttggcg ttggcttcgc
     2701 ccaattcccg acactgaagg aaaccgaaga cttttatgaa tttgaatggg ccccgattgg
     2761 attccgcgcg gcggtcgaat tctagagtga gctggtgcct gtcttgacag ttgacagatt
     2821 cagcaacgag tcccggggca atgcggcaag tgcca
//
LOCUS       DAOTFD010000289         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_28338_length_2813_cov_3.768673, whole genome shotgun sequence.
ACCESSION   DAOTFD010000289 DAOTFD010000000
VERSION     DAOTFD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28338_length_2813_cov_3.768673"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5079_11320"
     CDS             complement(<1..214)
                     /locus_tag="P5079_11320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRY30610.1"
                     /translation="MSPRFSIIIPVKGINDYIRENVSYILKLNSPDWELIILPNDASP
                     SEWSDGRISVVPTGRVGPADKRDVGAR"
     gene            complement(211..1707)
                     /locus_tag="P5079_11325"
     CDS             complement(211..1707)
                     /locus_tag="P5079_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY30611.1"
                     /translation="MKISISYPPLASSKGVPLLSQNRQFQWFKSPTYIYPVIPAYAAT
                     LLKSRGFDVVWDDGIAEELTGEAWFQRLQRTDPNVIVLESKTPVIKLHWRLIEKIKGW
                     KPAVKVVLVGDHVTALPQESMEKSAADYVITGGDHDFVLADLCAHLAGKTPALPGGVW
                     YRGNGAVLNTGLADLNHDLNALPFIDRRLTRWDLYAHKNGNFKYTPGTYTMAGRDCWW
                     GRCTFCSWTTLYPGKTFRTVSPERHVEEIGRLIADLNVREIFDDSGCFPKGEWLEKFC
                     HGILQKGYNKRVTLGCNMRVGALRQEHWQLMKKAGFRFILIGLESVVQSTLDRLDKGI
                     RVAQIEETVRMAKEAGLEPHITTMVGYPWETKEQSEQTIDFAKRLFRKGYLDTLQATI
                     VVPYPGTPLFEESRKNGWLLTEDWDHYDMKQSVWKSPISNEDVLKLTQGLYKAALNPA
                     FILRKILSIRNADDLKYLAHAGKKLIAHLMDFHGRSPKADDCSSPDDK"
     gene            complement(1709..>2813)
                     /locus_tag="P5079_11330"
     CDS             complement(1709..>2813)
                     /locus_tag="P5079_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30612.1"
                     /translation="LSVPVSTLLMVVTRKIAEFKAQNDLAGIGGLYRRINNRVLVAGF
                     FALVVFVLCASPIQSYLHVPAVMPVILLGVLIFTTLPYPVNMAVLQGVQDYTWININQ
                     GLAGPAKFIFAVGFVLVGFRVNGVMAGLILTSLVLWLSSFYPIRGFALNRDRAAASPD
                     RIAFAEVLPIFFAVFAFAVLTQADMVLVRRYFLPHDTGLYASAAILGKAVMYVPGAVV
                     LAMFPMVSEQRALNQSSRHVLAKSIAITLALSGAGALLFFLCPGWIMRTVYGVRYSEA
                     QSVLKYYGVAMLPMALLMVLMQYLIARKKSLFSYIMIVGGGLEIAAISLMHRSLLQVV
                     FTMLVVGAALFGVGVAIQYVPSLQSESPATREA"
BASE COUNT          543 a          833 c          918 g          519 t
ORIGIN      
        1 accgcgctcc cacgtcgcgc ttgtccgccg ggccgactcg cccggtcggg accacgctga
       61 tgcgcccgtc gctccattcc gacggcgacg cgtcgttcgg caggatgatc agttcccaat
      121 cgggggagtt cagtttgagg atataggaga cgttctcccg gatgtaatcg ttgatgccct
      181 tgaccgggat gatgatcgaa aaacgcgggc tcatttatcg tcgggagagg agcagtcgtc
      241 tgcttttggc gaacggccgt ggaaatccat gaggtgggcg atcagcttct tgccggcatg
      301 ggccaggtat ttcagatcgt ccgcgttccg gatcgacagg atcttccgga ggatgaacgc
      361 cgggttcaac gcggccttgt agaggccttg cgtgagtttc aggacgtctt cattgctgat
      421 cgggcttttc cacacggact gtttcatgtc gtagtggtcc caatcctccg tgagcagcca
      481 gccgttcttc ctggattcct cgaacagcgg cgtgcccgga tacggaacga cgatggtggc
      541 ctgcagcgtg tcgagatagc ctttgcggaa aagacgcttg gcgaaatcga tggtctgctc
      601 cgattgttcc ttggtctccc agggatagcc gaccatcgtg gtgatgtggg gctcgaggcc
      661 ggcttccttc gccatccgca cggtctcttc gatctgtgcc acccggatgc ccttgtcgag
      721 gcggtccagc gtggactgga cgacggattc cagtccgatg aggataaacc ggaacccggc
      781 ttttttcatg agctgccagt gttcttggcg cagggcgccg acgcgcatgt tgcagcccag
      841 ggtcacccgt ttgttgtagc ccttctggag gatgccgtgg cagaattttt caagccattc
      901 ccctttgggg aaacagccgc tgtcgtcgaa gatctcccgc acgttcaggt cggcgatcaa
      961 acggccgatc tcctccacgt gccgctccgg cgacaccgtc cggaaagtct ttccgggata
     1021 gagggtcgtc caactgcaga aagtgcaccg cccccaccag caatcccgcc ccgccatggt
     1081 gtaggtgccc ggcgtgtatt tgaagttccc gttcttgtgg gcatacaggt cccaacgggt
     1141 caggcgcctg tcgatgaacg gcagggcgtt gaggtcgtgg ttcaggtcgg ccagccccgt
     1201 gttcaggacg gcgccgttcc cgcgatacca gacgccgccc ggcagcgcgg gggtcttgcc
     1261 ggccaggtgc gcgcacaggt ccgccaggac gaaatcgtgg tccccgccgg tgatgacata
     1321 atcggcggcg gacttctcca tggactcctg cggcagggct gtgacgtggt ccccgacgag
     1381 cacgaccttg acggcgggct tccagccctt gatcttctcg atcaaccgcc agtggagttt
     1441 tatcacgggc gtcttgctct ccaggacgat gacgttcggg tcggtccgct gcagtcgttg
     1501 gaaccaggcc tcgccggtca gctcctcggc gatgccatcg tcccagacga cgtcgaagcc
     1561 gcggcttttc aggagcgtgg cggcgtaggc tgggatcacg ggatagatgt acgtcgggct
     1621 tttgaaccac tggaactgcc ggttctggga aagcagcggg acgcctttgg acgaggcgag
     1681 cggcggatag gatatggaga tcttcatgtc aggcctccct cgtggcgggc gattccgact
     1741 gcaggctcgg gacatattgg atggccacgc cgacgccgaa cagcgccgcg cccacgacga
     1801 gcatcgtgaa gaccacctgc aaaagggacc ggtgcatcag gctgatggcc gcgatctcga
     1861 gcccgccgcc gacgatcatg atgtaggaga acagcgattt tttccgggcg atcaaatact
     1921 gcatcagcac catgaggagc gccatcggca acatggcgac gccgtaatat ttcaggaccg
     1981 actgcgcctc gctgtagcgg accccataga cggtgcgcat gatccagccc gggcacaaga
     2041 aaaaaagaag ggctccggcg ccggacaggg ccagcgtgat ggcgatcgat ttggccagca
     2101 cgtggcggct gctctggttg agcgcccgct gttcgctcac catggggaac atggccagga
     2161 cgacggcgcc ggggacatac atgacggcct tgccgaggat cgccgcggag gcgtacagcc
     2221 cggtgtcatg cgggaggaaa taccgccgga cgagcaccat gtccgcctgg gtcaggacgg
     2281 cgaacgcgaa gacggcgaag aagatcggca ggacctccgc gaaggcgata cggtccggcg
     2341 acgcggccgc ccggtcccgg ttaagcgcaa aaccgcggat cgggtaaaag ctcgacaacc
     2401 acaacaccag actcgtcaag ataagaccgg ccatgacgcc gttcacgcgg aagcccacga
     2461 gcacgaaccc caccgcgaaa atgaatttcg ccggaccggc cagcccctgg ttgatgttga
     2521 tccacgtata gtcctggacc ccctgcagga cggccatgtt gaccggatag gggagtgtgg
     2581 tgaagatcaa gacgccgagg aggatgaccg gcatgaccgc cgggacgtgc agatacgact
     2641 ggatgggcga cgcgcaaagg acgaagacga cgagggcgaa aaaaccggcg acaaggacgc
     2701 ggttgttgat ccgacggtaa aggccgccga tgccggccag gtcgttttgg gccttgaatt
     2761 ccgcgatctt ccgcgtgacg accatcagca gggtggagac cgggacggag agg
//
LOCUS       DAOTFD010000290         2796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_28589_length_2796_cov_4.958409, whole genome shotgun sequence.
ACCESSION   DAOTFD010000290 DAOTFD010000000
VERSION     DAOTFD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28589_length_2796_cov_4.958409"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1148
                     /gene="mltG"
                     /locus_tag="P5079_11335"
     CDS             63..1148
                     /gene="mltG"
                     /locus_tag="P5079_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY30613.1"
                     /translation="MVEENGSSSAPRPAAPHRRWGRKVLLAGLVVAVGAVLWINSAPA
                     TSHDEFFSVPSGASASRVTALLAESGQIRSRRWFLWLVGFRKAAGRLRAGVYEIERGQ
                     SAWGILENFLRGNTRRIRVTVPEGFASWQIAERLEANDICGAEDFKAMASSAAAEGTL
                     FPETYHLEQGMPPAEVLRLMREQFQKNWDEALSAAVSSGSVRQVVKSTSSSEERIQLA
                     DGRWWTRRQAVTMASLIEREARLPAERPLVSAVYHNRLKKRIPLEADPTVQFALGYWK
                     ERVLFRDLEVDSPYNTYRHYGLPPGPICSPGRAALAAALSPASVDYLYFVADQQGGHR
                     FSVKFDEHKKEVRNRNRERRLKKTGKR"
     gene            1158..2111
                     /locus_tag="P5079_11340"
     CDS             1158..2111
                     /locus_tag="P5079_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30614.1"
                     /translation="MNSKRYFFGCALLLAASAVESWAGVNLNGYALRGTKGYQERVLE
                     AILRLEPKVDWDDPDTPWPFSAYWLRGTRIGYDRDGASGPSYNTEGEAGIQLFGRSAV
                     AFRYGVTPALSEDVGNYRSRHWAMDVTAGWEGWYPQLAIDRPRWGTGVESRLTMTIGR
                     KTHEERLQVSSRTVRWMELREDVLGAVFSQTYGGATTVHVGGMAHNYDEDIHRLALRG
                     DYLALQNTVTAAPIGLLQGFVKNEWGAGVEQYLGKYVKLSLDWQRASYEAGLIERSDT
                     LRAGVTVFLGLYFAVNGVYETFNPQKQADSSYKGVGLGFRF"
     gene            complement(2122..>2796)
                     /locus_tag="P5079_11345"
     CDS             complement(2122..>2796)
                     /locus_tag="P5079_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY30615.1"
                     /translation="IVLLLTQSRGPWLGALLGLFLIAVFHPKRLWLTPPMLLLAAMMF
                     LSPALSSRAATLTEKHTDQSTVTRVVLWQDGLYVAKRHWLSGAGPDQLRNATERYRTE
                     KKFPPNPYGFESDMHNQYLQHLAERGVIGLAALLWLMGVPLWKSWAVYRRERKDLPGN
                     TEGAAVSLALLACFSSFFVVNLTERAFDDAEVALVFWLMAALTASKTEEKIPAAQHFP
                     VEPQNS"
BASE COUNT          522 a          853 c          889 g          532 t
ORIGIN      
        1 aaaacgcgac caggccgcgg cgtgtctcat cctgcaaaac tacctcgatg ccaaacgaaa
       61 aaatggttga agaaaacgga tcttcgtcgg cccctcgccc cgccgcgccg catcggcgtt
      121 gggggcggaa ggttctcctg gccgggctcg tcgtcgccgt cggtgccgtg ctctggatca
      181 attccgcgcc cgccacatcc catgatgagt ttttttccgt gccgtccggc gcttccgctt
      241 cccgggtgac ggccctgctg gcggaatcgg gtcagatacg ttcccgtcga tggttcttgt
      301 ggcttgtcgg tttccggaag gccgccggcc ggctccgggc cggcgtttat gagatcgaac
      361 gcggccagtc cgcctgggga atattggaga atttcctcag aggaaacacc cgccgtattc
      421 gcgtcaccgt tccggaaggt ttcgcttcct ggcagatcgc cgaacggctg gaggcgaacg
      481 acatttgcgg cgcggaggat ttcaaggcga tggcgtcgtc ggccgcggcc gaagggaccc
      541 tcttcccgga gacgtatcac ttggaacagg ggatgcctcc cgccgaggtg cttcgtctca
      601 tgcgggagca gttccagaaa aattgggacg aagcgctgtc ggcggccgtt tcttccggca
      661 gcgtccggca ggtcgtgaag agcacgtctt cttcggaaga gaggatacaa ctggccgacg
      721 gccgctggtg gacccggcgc caggccgtga ccatggcctc gttgatcgaa cgcgaagccc
      781 gcctccccgc cgaacgtccc ctcgtctccg ccgtgtacca taaccgcctg aaaaagcgta
      841 ttcccctgga ggccgatccc acggtgcagt tcgctctcgg ctattggaaa gagcgggtcc
      901 ttttccggga tttggaggtg gacagcccct acaataccta ccgccattac ggtctgccgc
      961 ccggccccat ttgcagcccc ggccgggccg ccctggccgc ggccttgtcg cccgcctctg
     1021 tggactattt gtatttcgtg gccgatcagc agggagggca ccggttttcc gtgaagttcg
     1081 acgaacataa aaaagaagtg aggaacagaa atcgtgagag acgcctcaaa aagacgggaa
     1141 agcgctagaa taggaccatg aattcaaaaa gatatttctt cggatgcgcc ctcctcctgg
     1201 cggcttccgc cgtcgagagc tgggcgggag tgaacttgaa cgggtatgcc ttgcggggaa
     1261 ccaaaggata ccaggagcgg gttttggaag cgatcctgcg gttggagccg aaagtggatt
     1321 gggacgatcc cgacaccccc tggccgttca gcgcgtactg gctccggggg acccgcatcg
     1381 gatacgaccg cgacggcgcc tccggacctt cctacaacac cgagggggag gcgggaatcc
     1441 agttgttcgg ccgctcggcg gtggcgttcc gttatggcgt caccccggct ctgtcggaag
     1501 atgtcggcaa ctaccgttcc cgccattggg ccatggacgt gaccgccggc tgggaaggat
     1561 ggtatcccca gttggcgatc gaccggcctc ggtgggggac gggcgtggaa agccgtctca
     1621 ccatgacgat cggccggaaa acccatgagg aacggctcca ggtcagctcc cgcaccgtac
     1681 ggtggatgga gctgcgggaa gatgttttgg gcgccgtttt ttcacagacc tacggcgggg
     1741 ccaccaccgt tcacgtgggc ggcatggcgc ataactacga cgaggacatt caccggctcg
     1801 cgttgcgtgg ggactatctc gcgctccaga acaccgtcac cgccgcgccg atcgggctgc
     1861 tccaggggtt cgtcaaaaat gaatgggggg ccggcgtgga gcaatacctc gggaaatacg
     1921 tcaagctctc gctggattgg cagcgggcct cctacgaggc ggggctcatc gaacggagcg
     1981 acacgcttcg cgcgggcgtg accgtgttcc tcggccttta tttcgcggtg aacggcgtgt
     2041 acgagacctt taatccgcag aaacaagcgg actcctccta taaaggggtc ggcctggggt
     2101 tccgttttta gcgagctgtt ttcaggagtt ttggggctcg acggggaaat gttgagcggc
     2161 gggaattttt tcttcggttt ttgacgccgt cagcgccgcc atcagccaga acaccagcgc
     2221 cacctccgcg tcgtcgaacg cgcgctcggt gaggttcacc acgaaaaacg aggagaaaca
     2281 cgccaggagg gccagcgaca ccgcggcccc ctcggtgttt cccggcaaat ccttccgttc
     2341 ccgacgataa accgcccaac ttttccacag cggaaccccc atcagccata acaacgccgc
     2401 cagaccgatg acaccgcgtt ccgccagatg ctggaggtat tggttgtgca tgtccgactc
     2461 gaacccgtaa gggttcggag gaaacttttt ttctgtccgg tatcgctccg tcgcgttccg
     2521 cagctggtcc ggcccggccc cgctcaacca gtgccgtttc gccacgtaga ggccgtcctg
     2581 ccagagaacc acccgcgtca ccgtgctctg gtccgtgtgt ttttcggtca gcgtggcggc
     2641 ccgggaactc agggcgggac ttaagaacat catggctgcc aagagcagca tggggggcgt
     2701 tagccacagg cgtttcgggt ggaacaccgc aatgaggaac aggccgagga gggcgcccag
     2761 ccaggggccc cggctttgcg tcaacagcag gacgat
//
LOCUS       DAOTFD010000291         2710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_29981_length_2710_cov_4.257250, whole genome shotgun sequence.
ACCESSION   DAOTFD010000291 DAOTFD010000000
VERSION     DAOTFD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29981_length_2710_cov_4.257250"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2311)
                     /locus_tag="P5079_11350"
     CDS             complement(<1..2311)
                     /locus_tag="P5079_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRY30616.1"
                     /translation="MKNNLSALFSEKILVLDGAMGTEIQKFRLTAKDFGGAPFEGCNE
                     HLLLTRPDVISSVHEGYLKAGADVIETNTFGTTAIVLAEYGLQDKAYELSKAGARLAK
                     EVAGKYSTSAKPRFVAGSMGPTTKSLSVTGGVTFDSLAEAFAEQARGLLDGGVDMLLI
                     ETAQDTLNIKAALVGVDAAFASTGHKVPVSVSATIELMGSMLAGQDVEALYYSLQQRD
                     LFTIGINCATGPDFMTDHVRALAEISRFGVSCVPNAGLPDEEGRYNETPEMIAKKLER
                     FAENGWVNILGGCCGTGPEHIRLIAQMADGKKPRRVRPAPIFSLSGIEPFVPDEDQRP
                     VLVGERTNVIGSRKFREMIVAGQFEEGTEIARRQVKSGAQIIDVCLANPDRDEKSDMG
                     HFAALLAKKIKVPLMIDSTDPAVMETALKLCMGRSVINSINLEDGESRFEKVVPLLKK
                     YGAAVVVGCIDEDKQQGMAVKRERKLAVAERSHRLLTEKYGLVPEDLVFDPLVFPAAT
                     GDKNYIGSARETMEGVRLIKQRFGGAKTILGISNVSFGLPEAGREVLNSVFLHHCVQA
                     GLDMAIVNAEKLARYPSIPENEKQLAEKLLFASVADYDAVVAEFTAHFRAKVAVKKEV
                     ILGGTPDERLVRNVLEGSKEGLVEDLEALLQGGRNPLEIVNGPLMKGMGEVGKLFAKN
                     EMIVAEVLQSAEVMKAAVTYLEPKMEPGAVSKRGKIVLATVKGDVHDIGKNLVHIIFK
                     NNGFAVVDLGIKVPPEEIIAAAFGGDEAEA"
BASE COUNT          516 a          850 c          763 g          581 t
ORIGIN      
        1 gggcttcggc ttcatcaccc ccgaaggcgg cggcgatgat ctcttccggc ggcaccttga
       61 tgccgaggtc cactacggca aaaccgttgt tcttgaaaat gatgtgcacc aagtttttcc
      121 cgatgtcgtg cacgtcgccc ttcaccgtgg ccaaaacgat cttcccccgt ttggaaacgg
      181 cgccgggctc cattttcggc tccaaatacg tcacggcggc tttcatcacc tccgcgcttt
      241 gcaggacctc ggccacgatc atctcgttct tggcgaaaag ttttccaacc tctcccatcc
      301 ccttcatgag ggggccgttc acgatctcca ggggattccg tcccccctgg agcagggcct
      361 ccaggtcctc gacgagcccc tccttggaac cttccagcac gttgcggacc aaccgctcgt
      421 cgggcgtgcc gccgaggatt acctcttttt tgacggcgac cttggcgcga aaatgcgccg
      481 tgaactccgc caccaccgcg tcgtagtcgg cgacggacgc gaacagcaat ttctccgcca
      541 actgtttttc gttttcgggg atggacggat accgagccag cttctccgcg ttcacaatcg
      601 ccatgtccag ccccgcctga acgcaatgat gcagaaaaac ggagttcaag acctcgcggc
      661 cggcttccgg caggccgaac gacacgttgc tgatgcccag gatggtcttt gccccgccga
      721 accgttgctt gatgaggcgc accccctcca tggtctcccg ggcggagccg atgtaattct
      781 tgtctccggt ggcggcggga aacacgaggg ggtcaaagac cagatcttcc ggaaccaacc
      841 cgtatttctc cgtcaagagg cggtggcttc tctcggcgac ggccagcttg cgctcccgtt
      901 tcacggccat gccctgctgc ttatcctcgt cgatacagcc caccaccacg gccgcgccgt
      961 attttttcag aagcgggacg actttttcaa aacgggactc gccgtcttcc aggttgatgg
     1021 agttgatgac ggaccggccc atgcacagct tcagcgcggt ctccatcacg gcggggtcgg
     1081 tggaatcgat catcagcgga acttttattt ttttggccaa aagggccgcg aaatggccca
     1141 tgtcggattt ttcgtcgcgg tcggggttcg ccaggcacac gtcgatgatc tgcgcgcccg
     1201 acttcacctg ccgacgggcg atctccgtcc cctcctcgaa ctgtcccgcg acgatcatct
     1261 ccctgaactt gcggctgccg atgacgttgg tgcgctcgcc gacgagaacc ggtctctggt
     1321 cctcatccgg aacgaacggc tcaatgccgg aaagcgagaa aatcggcgcg gggcggaccc
     1381 ggcgcggctt ctttccatcc gccatctgcg cgatgagccg gatatgttcg gggccggtcc
     1441 cgcaacagcc gcccaaaata ttcacccaac cgttttccgc gaaacgttcc agtttttttg
     1501 cgatcatttc cggtgtttcg ttgtagcggc cctcctcgtc cggcaggccg gcgttgggca
     1561 cgcaggacac gccgaaccgc gagatttccg ccaaggcgcg gacatggtcc gtcatgaaat
     1621 ccggcccggt ggcgcagttg atgccgatag tgaacagatc tctctgctga agggaataat
     1681 agagggcctc cacgtcttgg cccgccagca tggatcccat gagctcgatc gtcgccgaca
     1741 ccgaaacggg gactttgtgg ccggtcgagg cgaacgccgc gtccacgccg acgagcgccg
     1801 ccttgatgtt gagcgtgtct tgggccgttt caatgaggag catgtcgacc ccgccgtcca
     1861 acaagccgcg ggcctgctcg gcgaacgcct ccgccagcga gtcgaacgtg acccctcccg
     1921 tcaccgagag acttttggtg gtgggcccca tggaaccggc cacgaaacgc ggcttggcgg
     1981 atgtggaata tttaccggca acctctttgg ccagccgggc gccggccttg ctcagttcgt
     2041 aggctttgtc ctgcaggccg tattcggcaa ggacaatggc ggtggtgccg aaggtattgg
     2101 tttcaatgac gtcggcgccg gccttcaggt atccttcgtg aacagacgaa atcacgtccg
     2161 gccgggtcag caggaggtgt tcgttgcacc cctcgaacgg cgccccgccg aaatctttgg
     2221 ccgtgaggcg gaatttctgt atctccgtcc ccatggcccc gtccaagaca aggattttct
     2281 ccgaaaaaag cgcgcttaaa ttatttttca tacccgttaa gattacataa ttctaacgac
     2341 ctttttaaga aaaggtcgcc gtgcgcatta aaacctctcc acaagccgaa tacgcgatgt
     2401 gataagattg ttgagaacaa aacaccttac gaggtgccac gaaattggaa atattccgac
     2461 tctttccgtt cacatggatc cgctcaaaac accgctggct tctggcggga cttgcgctgg
     2521 ccgtctcatt ggctgtcgcg gaatgggcct ttcgaaggac ccagtcaaaa gaactcatgc
     2581 cgtggcggca tccggaaccc tacgtgatgt tctccaagga gctcaaatcc ctggccggcg
     2641 acacgtatcc tcacgaattc aaggtattca tgctgggggg ctccacggtg gcgatggggg
     2701 gatgggttcg
//
LOCUS       DAOTFD010000292         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_30478_length_2683_cov_4.818493, whole genome shotgun sequence.
ACCESSION   DAOTFD010000292 DAOTFD010000000
VERSION     DAOTFD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30478_length_2683_cov_4.818493"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5079_11355"
     CDS             complement(<1..605)
                     /locus_tag="P5079_11355"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30617.1"
                     /translation="MTEPSKEKNITRISGLPGGSEAWWLLQNRRAHPRWLVLAATDEA
                     AQNLFDDFQALERWQTPAAPSEAAYFSEEEEDVRTAALSAWSRSGPEAARFLFSSWES
                     AQRGLPVPDDFRTGHVALRIGDKKDRSAFLLHLSQQSYSRVDSVEQVGEMAVRGEVVD
                     LWSPGWDGPMRFLWPFDQVESIRKIDLATQRSTDAVETVLVR"
     gene            complement(1413..>2683)
                     /gene="rlmD"
                     /locus_tag="P5079_11360"
     CDS             complement(1413..>2683)
                     /gene="rlmD"
                     /locus_tag="P5079_11360"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRY30618.1"
                     /translation="SRYWIESLQEQSPHRAAPPCPYFFRPGAAPAAVCGGCDWQHLSY
                     DYQLLTKRKLVEEALQRIGRIGKPPVSDTVPVNSPAVAPADAKRGDGSAWRYRNKVQI
                     PFTERDGRLTAGFYAPGSHHIVPFEDCLVQTEESVRVFRAVREWFLKNPVQAYDQDAR
                     RGWLRHLLVRTNAKGEALAALVTNGPSFPHAVEFSRHLTAACPFVVSLFQNVNVHPGN
                     VVLGPEWRHLHGKRYLAEEMLRLKFRLSPGSFFQVHHAMAEKLYRMVESFAAPNPQES
                     VLELYAGVGAIAQMLAKNSRFVWAVEENPQAVEDAIASAGWNNLRNVRFLLGRCETSL
                     ARGRFRKGMSDRLGTVVLDPPRAGCEQHVLRALMRLEPKKIVYVSCDPATLARDARYL
                     VTGGYHLRRCTPVDLFPQTSHIESVSEFTK"
BASE COUNT          496 a          803 c          861 g          523 t
ORIGIN      
        1 cggaccagga ccgtttccac cgcgtcggtg gaccgctggg tggccaggtc gatcttccgg
       61 atggattcca cctggtcgaa cggccacagg aaccgcatcg ggccgtccca gccgggggac
      121 cagaggtcca ccacctcgcc gcgcaccgcc atctcgccga cctgttccac ggagtccacg
      181 cggctgtagg attgttggga gaggtggagc aggaaggcgg agcggtcctt tttgtcgccg
      241 atccgcaggg cgacgtggcc cgtgcggaaa tcgtccggga ccggaaggcc ccgttgggcc
      301 gattcccagg aggagaagag gaaccgggcc gcctccgggc cggaccgcga ccaggcggag
      361 agcgcggcgg tgcgcacgtc ctcctcttct tcggagaaat aggcggcttc ggagggggcg
      421 gcgggggtct gccagcgttc cagcgcctgg aaatcgtcga agaggttctg cgccgcctcg
      481 tcggtggcgg ccagcaccag ccagcgcggg tgggcccggc ggttttgcag cagccaccac
      541 gcctcgctgc cgccggggag gccggagatg cgcgttatgt ttttttcttt tgacggttca
      601 gtcaaccggt ttctccctta tttgcccatc gtttcgatcc ctcaaacagc aaaggccttc
      661 tcccttttaa tggggggaag gtgcggatga cagggatttt atcttatcgc agggcttacc
      721 gccctgcacc tgtgtcccct ttctttgctc gtacaaagaa agggaaccga aagaaatacg
      781 cccctgcggg caatccgtcg gcgaatttcc ggcggccgtg aactcatccc tttcaaacgc
      841 cgtgaaaggg attcaaacat cacggcctgt cagctccgaa aattcgcctc cttcaaaacg
      901 cccttaaggg gggtattatc aaaagatttc agaaccccct gagccggtta tgagccgagg
      961 cgattttcgg acagtagaca ccggaggatg tttgagcagg ccgtcggaca ttaaggcctg
     1021 tgagttccgc cggtgccgaa aatcgcggag cgaatggcga taaggggtgt cgttcttttg
     1081 ctgacttttc ttggacaagc aagaaaagtc agttgccgcc gggcaatccc ggcgccgttt
     1141 atttaaaagc gggaataaga gtggctatga caagtccgac ggttcccccg acaaggcagc
     1201 aagttgccca aacaagctgt tttggggatt tttccattcg atcggccagc ctatgttgaa
     1261 aaatcaatgt tgacgcaaga gaccggatat cgggattttt tgcccaatcg ccggtttttt
     1321 cgaaagattt tttttgcttc gctgaattct tgccatttct catcagtatg gatatcgctc
     1381 attattcttc ctcaatacaa atcaaaatca atttattttg tgaactcgga cacgctctcg
     1441 atgtgggacg tctgcgggaa gaggtccacc ggggtgcagc ggcggaggtg gtagccgccg
     1501 gtgacgaggt agcgggcgtc ccgcgccagg gtggccggat cgcaggacac gtacacgatc
     1561 tttttcggct ccaaccgcat cagcgcccgc agcacgtgct gttcgcatcc ggcccgcggc
     1621 gggtccagca ccaccgtgcc cagccggtcg ctcatgcctt tgcggaaccg gccccgcgcc
     1681 agcgaggttt cgcatcggcc gaggaggaac cgcacgttgc gcaaattgtt ccacccggcg
     1741 ctggcgatgg cgtcttccac ggcctgcggg ttttcctcca cggcccacac gaaacgggag
     1801 ttcttcgcca gcatctgcgc gatggcgccc acgcccgcgt acagttccaa aacgctctcc
     1861 tgggggttcg gcgccgcgaa actttccacc atccggtaaa gtttctccgc catggcgtgg
     1921 tgcacctgga agaacgagcc cggcgaaagc cggaacttca ggcgcagcat ctcctccgcg
     1981 agataccgtt ttccgtgcag gtggcgccat tccggcccga ggacgacgtt gccgggatgg
     2041 acgttcacgt tctgaaagag gctgaccacg aaagggcagg cggcggtgag gtggcgggag
     2101 aactccaccg cgtgggggaa agaggggccg ttggtgacca gggcggccaa cgcctcgccc
     2161 ttggcgttgg tgcggaccag cagatgccgc agccagccgc gccgcgcgtc ctggtcgtag
     2221 gcctgcaccg ggtttttcag gaaccattcc cgcaccgccc ggaagacccg cacggattcc
     2281 tccgtctgca ccagacaatc ttcaaaaggc acgatgtggt gcgagccggg ggcgtaaaaa
     2341 ccggcggtga gtcggccgtc ccgttccgtg aacgggatct gcaccttgtt gcggtaccgc
     2401 caggccgacc cgtctccgcg tttggcgtcc gcgggcgcga cggcggggga gttgaccgga
     2461 acggtgtccg acaccggcgg ttttccgatg cggccgatgc gctggagcgc ctcctccacc
     2521 agtttgcgtt tggtcaggag ctggtagtcg taggagaggt gctgccagtc gcagccgccg
     2581 cagacggcgg cgggcgccgc tcccggcctg aagaaatacg ggcagggcgg ggccgcgcgg
     2641 tggggggatt gttcctgcag ggactcgatc caataccggg aaa
//
LOCUS       DAOTFD010000293         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_30640_length_2674_cov_3.704086, whole genome shotgun sequence.
ACCESSION   DAOTFD010000293 DAOTFD010000000
VERSION     DAOTFD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30640_length_2674_cov_3.704086"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="P5079_11365"
     CDS             complement(<1..744)
                     /locus_tag="P5079_11365"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRY30619.1"
                     /translation="MNWKNRSVLVTGAGGFIGSHLTEQLLRRGASVRALVRYNSRNDW
                     GFLEEIGPELRKRLDVWSGDVADPHFMLNAVEGRDVVFHLAALIAIPYSYIAPSIYAA
                     TNVTGTLNVLEACRRQRVKRVVHTSTSEVYGTACYTPIDEKHPLQGQSPYSASKIGAD
                     KLAESYFRSFGLPVVTVRPFNTFGPRQSARAVIPTIITQLLSGAKKLSLGSLNPVRDF
                     NFVLDTVEGFIAAGGAPRVEGEVINVGSGR"
     gene            complement(894..1901)
                     /locus_tag="P5079_11370"
     CDS             complement(894..1901)
                     /locus_tag="P5079_11370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30620.1"
                     /translation="MTSNQLISIIIVNYNGKRWLKTCLDSVLAQTYKNIEIILVDNAS
                     SDDSVAFVSGAYPQVQIVRNPENHGFGKANNIGAARARGDVLFLLNNDTKSELDLLER
                     LLQFKVERRLSIVGPKILDYEGKDIYEGKKVTIDVTGYLGWGGHTFYIEGSAFMISKA
                     DFNKLRGFDEKYFMYSEDIDLCWRALISGMTLGVCDTAMVSHYCGGTSAKSVVRSEEA
                     SDARHVIPVFRRYEAEKNNLRNLLKNYSWMNLLWAVPLALFFVFGESVFYLFTRNFRM
                     CGMIWKSVLWNVLNIGDTWRERRLVQGGRTVGDGLILKMMNFKPNKVRALLHVGIPEF
                     K"
     gene            complement(1928..>2674)
                     /locus_tag="P5079_11375"
     CDS             complement(1928..>2674)
                     /locus_tag="P5079_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30621.1"
                     /translation="LTARDSTDLTLGDFIVKEALQQMDTVKPPYVVLNMHTAHGMVTM
                     GAKREDVGACLSLGTPDSPCFAGTGTEMEFYILRNYELAERFDYAAILKRREHPVDFS
                     RKFKTNFSYDGNQTERPAMEITGLAETERPGVFKITQRTATIVFPFTDPRDALYINTD
                     FIISAGPLLKLLSKARVSVKAEFEPAGENASGVYFDLLKLGTVQSVGVGFQNQVVRVK
                     KLTILIETPAPYLLPDRFELKGVQFTELIP"
BASE COUNT          582 a          774 c          726 g          592 t
ORIGIN      
        1 ccggcccgaa ccgacgttga tgacttcccc ctcgacgcgc ggcgcgccgc cggcggcgat
       61 gaagccttcc accgtgtcga ggacgaaatt aaaatccctc acggggttca gcgatcccaa
      121 cgatagcttc ttagcccccg acaacagctg cgtgatgatg gtggggatca ccgcccgggc
      181 ggattggcgc ggaccgaacg tattgaaggg acggacggtg acgacgggta acccgaacga
      241 ccggaaatag ctctccgcca gcttgtcggc cccgatcttg gaagccgagt agggagactg
      301 gccctggagc gggtgttttt catcgatggg cgtataacag gccgtcccgt agacttcgga
      361 ggtggacgtg tggaccaccc gcttgacgcg ctgccgacgg cacgcctcca ggacgttgag
      421 ggtccccgtg acgttcgttg cggcgtagat ggacggagcg atgtacgagt aagggatggc
      481 gatcaacgcc gccaggtgaa agaccacgtc gcggccttcc acggcgttga gcatgaaatg
      541 cgggtcggcg acatcgccgg accagacatc gagccgcttc cggagttcag ggccgatctc
      601 ttccaaaaat ccccagtcgt tgcgtgagtt ataccgcaca agagcgcgca cgctggcgcc
      661 ccgccgaagg agttgttcgg tcaaatgact gccgatgaac ccaccggcgc cggtgacgag
      721 gacagacctg tttttccagt tcattttcat gttcgtttag ggacgaaaac cctttctttg
      781 acgctgtttg acacgactaa gaataccata ttcccgtgtg cagtaaaatt gcatggccgg
      841 gtgtttttct gcccgggttc caagttcccc ggctggccca aaacacccta ggactatttg
      901 aattcaggga tgccgacgtg caacaaggca cgcactttat tgggcttgaa attcatcatt
      961 ttcaggatca aaccgtcccc cacggtccgt ccaccctgca cgaggcggcg ttctctccac
     1021 gtatcgccga tgttcaaaac gttccataga accgatttcc atatcattcc gcacatcctg
     1081 aagttcctcg tgaacagata gaacacggat tccccgaaga cgaagaacag agccaagggg
     1141 accgcccaca acaggttcat ccaggaatag ttcttcaaaa gattccttaa attgtttttt
     1201 tcagcctcgt accgccggaa gacggggatc acgtgacggg catccgacgc ctcctccgat
     1261 ctgacgaccg attttgcgga tgtccctccg caataatgcg agaccatcgc cgtgtcacag
     1321 acccccagag tcatcccgga gatcagagcc ctccagcaca agtcgatgtc ttcactgtac
     1381 atgaaatact tctcgtcgaa cccgcgcagc ttgttgaaat cggccttcga tatcatgaag
     1441 gcggacccct cgatgtagaa ggtgtggccg ccccagccca ggtaaccggt cacatcgatc
     1501 gtcacttttt tcccctcata gatgtctttc ccttcgtaat ccaatatctt gggaccgacg
     1561 atgctgagac gcctctcgac tttaaattgc aggagccttt ccaacaggtc gagctcggat
     1621 ttggtgtcgt tgttcaatag gaacagcacg tcgcctctgg ccctggccgc gccgatgtta
     1681 ttggctttcc caaaaccatg attttctgga ttccggacga tctggacttg cgggtaggcg
     1741 ccggacacga aagcgacgct gtcgtcggac gaagcgttgt cgacgagtat gatctcgatg
     1801 tttttatagg tctgagccaa taccgagtcc aagcaggttt taagccacct tttcccgttg
     1861 tagttgacga tgatgatcga gatcagttga ttagaggtca tctcttaaaa cgcaaatgac
     1921 ggatcactca tggtatcagc tcggtgaact gcacgccctt cagttcaaaa cgatcgggca
     1981 ataggtaagg cgccggcgtt tcgatgagga tggtcagctt tttgacccgg acgacctggt
     2041 tttgaaatcc cacccccacg ctctggaccg ttcccagctt cagcagatcg aaatacacgc
     2101 ccgaggcatt ctcgccggcg ggctcgaact ccgccttcac ggagacacgg gccttcgaaa
     2161 gcaacttcaa gagcggcccg gcgctgatga tgaaatccgt gttgatgtat agggcatctc
     2221 tggggtcggt gaacgggaag acgatggtcg cggtccgttg cgtaattttg aacacgccgg
     2281 gccgctccgt ctccgcgagt cccgtgatct ccatggcggg ccgctccgtt tggttcccgt
     2341 cataagaaaa attcgttttg aacttccggc tgaaatccac aggatgctcg cgacgcttca
     2401 agatggccgc gtagtcgaat ctctcggcca attcatagtt ccttaaaata tagaattcca
     2461 tttccgtgcc tgtgccggcg aaacagggac tatccggtgt ccccaaactc aggcaggcgc
     2521 ccacatcctc ccgcttcgca cccatcgtga ccatgccatg ggcggtgtgc atattcaaga
     2581 cgacgtaggg gggcttcacg gtgtccatct gctggagagc ttccttgacg atgaagtctc
     2641 cgagcgtcaa atcggtcgaa tcccgggccg tcag
//
LOCUS       DAOTFD010000294         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_31114_length_2650_cov_4.347977, whole genome shotgun sequence.
ACCESSION   DAOTFD010000294 DAOTFD010000000
VERSION     DAOTFD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31114_length_2650_cov_4.347977"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="P5079_11380"
     CDS             <1..665
                     /locus_tag="P5079_11380"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRY30622.1"
                     /translation="LYRFCEIARALLTGEHAVGRVIARPFKGKPGAFQRTESRRDFSL
                     KPFGPTVMDILKSQGRMVYAVGKIEDIFAAQGVTEAVHSHGNQEGMDDTLKAVKSRRQ
                     KGLIFTNLVDFDMLYGHRNDPRGYAQALKTFDDWLPKLMAEMTSSDLLIITADHGNET
                     TDVSTDHTREHVPLLVYGPAVRPGRDLGTRKTFADLGATVADLLDVPAPPVGSSFAPE
                     IF"
     gene            726..1535
                     /locus_tag="P5079_11385"
     CDS             726..1535
                     /locus_tag="P5079_11385"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRY30623.1"
                     /translation="MLPSAVEFLRSKTQGASAPGIAIVLGSGLGPVADTFTDPVVIPY
                     TDIPGFVGSTVAGHAGKLIFGRLSGKRIVAMKGRLHFYEGHSMEQLTFPYRVFKRLGI
                     ETLVLTSAVGGISPRLKAGDLMAVRDHINFMGENPLRGPHYDDYGARFPDMSEAYPKS
                     LRDIAKKAAKSLKFELKEGIYCAVRGPSYETPMEIQLFKKWGADVVGMSVIPETLISH
                     QEGVKVLAITCVSNKAAGLSGRPLSHEEVMETGRRVEKRFSGLIAKIVEML"
     gene            1561..>2650
                     /locus_tag="P5079_11390"
     CDS             1561..>2650
                     /locus_tag="P5079_11390"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRY30624.1"
                     /translation="MNAYDIIFKKRNGGELSREEIEFFVKGVTAGDLPPEQAAAWLMA
                     AFLKGLTKKEMVSLTTAMTHSGEILDLASVPGDKVDKHSTGGVGDGTSLVLAPLLSAS
                     GVVVPMMSGRALGHTGGTLDKLEAIPGFRVDLTADEFRRQLSSVGCAMIGQTAQVAPA
                     DKKLYALRDVTATVDSVTLIAASIMSKKLAEGTNALVLDVKTGSGAFMQKMKDARALA
                     AAMTAIGNGAGVKTVALITNMDQPLGGVAGNALEIRQAVEVLKGGGPADFCELILTLG
                     AWMLVLVKKARQPGEARKSLEPLLRDGRALEKFRDMVRAQGGDPAVADKPEALLPRAR
                     FQGAVPARRAGYVVKMDTRAIGMLVSGLG"
BASE COUNT          481 a          883 c          824 g          462 t
ORIGIN      
        1 aactttaccg tttctgcgag atcgcccggg ccttgctcac gggcgaacac gccgtgggcc
       61 gcgtcatcgc ccgtccgttc aaggggaaac cgggggcttt tcagcgcacc gagagccgcc
      121 gcgatttttc ccttaaacct ttcggtccca ccgtgatgga cattctcaaa tcccaggggc
      181 gcatggtcta cgccgtcggc aagatcgagg acatcttcgc cgcccagggc gtcaccgagg
      241 cggtccattc ccacggaaac caggagggga tggacgacac cctcaaggcg gtcaagagcc
      301 gtcgccaaaa aggcctgatc ttcacgaacc tcgtggattt tgacatgctc tacggccatc
      361 gcaacgaccc gcgcggatac gcccaggccc tgaagacctt cgacgactgg ctgccgaaac
      421 tgatggccga gatgacctcc tcggacctct tgatcatcac cgccgaccac gggaacgaaa
      481 ccaccgacgt ctccaccgac catacccgcg aacacgtgcc gctcctcgtc tacggccccg
      541 ccgtccgtcc gggacgggac ctgggcacgc gcaagacgtt cgccgacctg ggcgccaccg
      601 tggccgacct gttggatgtt cccgcgcccc ccgtcggctc cagtttcgcg ccggaaattt
      661 tctgatcacg ccgccggtgc cggcggcgca gcctatcgag ggagaacacc atgacattgc
      721 acgacatgct gccgtccgct gttgagtttt tgagatccaa gacccagggc gcttccgccc
      781 ccggtatcgc gatcgtcctc ggttccgggc tcggccccgt ggccgatacg ttcaccgatc
      841 ccgtggtcat cccctacacc gacatccccg ggttcgtcgg ttccaccgtg gccggccacg
      901 ccggcaaact gatcttcggc cgcctttccg ggaagaggat cgtcgccatg aagggccggc
      961 tccattttta tgaggggcac tccatggaac agctcacgtt cccttaccgc gtttttaaaa
     1021 ggttggggat cgaaacgttg gtcctcacct cggcggtggg aggcatctcc ccgcggttga
     1081 aggcgggcga cctgatggcc gtgcgggacc acatcaattt catgggagag aacccgctgc
     1141 ggggccctca ctacgacgat tacggcgcgc ggttcccgga catgtcggag gcctatccga
     1201 aatccctgcg ggacatcgcc aagaaagccg ccaaatcgct caagttcgaa ttgaaggaag
     1261 gcatctactg cgcggtgcgc gggccgtcct atgagacccc catggagata caactcttca
     1321 aaaagtgggg cgccgacgtg gtgggcatgt ccgtcatccc ggagacgctc atctcccatc
     1381 aggagggggt gaaagtcctg gccatcacct gcgtctccaa caaggccgcc gggttgtcgg
     1441 gccggccgct cagccacgag gaggtcatgg agaccggccg ccgggtggag aaacgttttt
     1501 ccggcctcat cgccaagatc gtcgaaatgc tttgatcgtt ttcggttgca tccctcccgc
     1561 atgaacgcct acgacatcat cttcaaaaaa cgcaacggcg gcgaactgag ccgcgaagag
     1621 atcgagttct tcgtcaaagg ggtcaccgcc ggcgatttgc cgccggaaca ggcggccgcc
     1681 tggctcatgg cggctttttt gaagggcctc accaagaagg aaatggtgtc gctcaccacc
     1741 gccatgaccc attcggggga gatcctcgac ctggcctccg ttcccggcga caaggtggac
     1801 aagcattcca ccggcggcgt gggcgacggc acgtctctgg tgttggcgcc cctgttgtcc
     1861 gcgtccggcg tggtggttcc catgatgtcg ggccgcgccc tgggccacac cggcggcacg
     1921 ttggacaagc tggaggccat ccccgggttc cgcgtggacc tcaccgccga cgagttccgc
     1981 cgccagctct cctccgtcgg ctgcgccatg atcggccaga ccgcccaggt ggccccggct
     2041 gacaaaaaac tctacgccct tcgggacgtc accgccacgg tggattccgt cacgctcatc
     2101 gccgccagca tcatgtcgaa gaaactggcc gagggaacca acgccctggt gctggacgtg
     2161 aagaccgggt cgggcgcgtt catgcagaaa atgaaggacg cccgcgccct ggccgcggcc
     2221 atgaccgcca tcggcaacgg cgccggcgtg aaaaccgtcg cgctcatcac caacatggac
     2281 cagccgctgg gcggggtggc cgggaacgcc ctggagattc ggcaggccgt cgaggtcctc
     2341 aagggcggcg gcccggcgga tttttgtgaa ctgattttga cgctgggcgc ctggatgctg
     2401 gtgctggtga agaaggcccg ccaacccggc gaggcccgca agagcctgga accgttgttg
     2461 agggacggcc gcgccttgga aaaattccgc gacatggttc gcgcccaggg cggcgatccg
     2521 gccgtggcgg acaaaccgga ggcgctgctg cctcgggccc gctttcaagg ggcggtgccc
     2581 gcccgccggg cgggatacgt cgtcaaaatg gatacccgcg ccatcggcat gctcgtgtcc
     2641 ggattgggcg
//
LOCUS       DAOTFD010000295         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_31520_length_2629_cov_4.608780, whole genome shotgun sequence.
ACCESSION   DAOTFD010000295 DAOTFD010000000
VERSION     DAOTFD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31520_length_2629_cov_4.608780"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5079_11395"
     CDS             <1..250
                     /locus_tag="P5079_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30625.1"
                     /translation="HMGRLDFDPLVKDPTLMGLVPRGRWGELLQRDDDVREENLLRYI
                     RSGRPAGDVGFVKKMEHLTGRKLACGRAGRPVKKKSNK"
     gene            complement(279..926)
                     /locus_tag="P5079_11400"
     CDS             complement(279..926)
                     /locus_tag="P5079_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY30626.1"
                     /translation="MKIGILGSGIVGVTLANGFIKHGYAVLLGTNDKTKYADLKTKTA
                     GKAGIGSFEEAAQFGEILVLAVRGRAAETVIKKVGLEKFKDKLVLDTTNPIADDEPVN
                     GVLKFFTSLDGSLMERLQRAAPSARFVKAFSCIGNAFFVNPDFGGVKPTMFICGNDAK
                     AKEQAVQILTQFGFEPADMGSVEAARAIEPLCILWCIPGFLQNDWNHAFKLLKKG"
     gene            1172..2284
                     /locus_tag="P5079_11405"
     CDS             1172..2284
                     /locus_tag="P5079_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY30627.1"
                     /translation="MFGSVMTGVVSLMHVYVFWRACTVPFVTARVPRKGVAALGLLMW
                     SVFFLGRVLGHGGTGPAAAALEFLGMNWMGALFLMTVTLLAADVLTLFGLVFKKYAPG
                     LRGAALLTGLALAAFALVQGLRPPVVRSDDVPLAGLPSALDGKVLVVISDLHLGSQTG
                     AGRLDAVVARAAAQKPDMVLLLGDITEGHDAPPETFLFSLRRLSAPWGVWGVLGNHDP
                     PRRAAPADGSLMEQAGIPVLRNAWVEVQPGLVLAGRDGRSVRRGGLPAEEALEKTLAG
                     RPAGATIFLSHYPDKAEAAARAGVGLMLSGHTHGGQVWPFGYLVRQRYPRLGGRYRIG
                     DMTLLVCRGTGTWGPRLRLWHPNEILRITLRRKNAE"
     gene            2437..>2629
                     /locus_tag="P5079_11410"
     CDS             2437..>2629
                     /locus_tag="P5079_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRY30628.1"
                     /translation="MELLAPVGSKESFITAIRGGAHAVYVGVPDFNARISASPLNLYD
                     LEVLIAHAHEKNVKVYLALN"
BASE COUNT          453 a          793 c          839 g          544 t
ORIGIN      
        1 tcatatgggc cggctcgatt tcgacccgct ggtgaaggat ccgacactga tggggctggt
       61 tccccgtggc cgatggggag aattgttgca gcgagacgac gatgtgcggg aggagaatct
      121 gcttcgttat attcgatcgg gaagaccggc gggggatgtg ggtttcgtca aaaaaatgga
      181 gcatctcacc ggccgcaaac tggcgtgtgg ccgcgcggga cgaccggtaa aaaagaagag
      241 caataaataa tctgtatact gtccccgaat tacccggatt accccttttt gagaagcttg
      301 aaggcgtggt tccaatcgtt ttgcagaaag cccgggatgc accacaaaat gcagaggggc
      361 tcgatggccc gggccgcctc caccgagccc atatccgcgg gttcgaaacc gaactgcgtg
      421 aggatttgaa cggcctgttc tttggctttg gcgtcgttgc cgcagatgaa catggtgggc
      481 ttaacgccgc cgaagtccgg attgacgaag aaggcgttgc cgatgcagct gaaagccttg
      541 acgaaacggg ccgacggggc cgcccgctgg aggcgctcca tcagggagcc gtccaacgag
      601 gtgaagaatt tcagaacgcc gttgaccggt tcgtcgtccg cgatggggtt ggtggtgtcc
      661 agtaccagtt tgtccttgaa cttttccaaa ccgacctttt ttatgacggt ttccgcggcg
      721 cggccccgga ccgccagaac caaaatctct ccgaactggg ccgcctcctc aaaggaaccg
      781 atgccggctt ttccggcggt cttggtcttg aggtcggcat acttggtttt gtcgttggtg
      841 ccgagcagga cggcgtagcc gtgcttgatg aatccgttgg ccaaggtcac ccccacgatg
      901 cccgaaccga gaatcccgat cttcatggaa acacccccct taattgatgc ctgcctgtgt
      961 tacgtctata acgttttttt cttcccccgg ttccgaaggt ttttcccgcc gaaaggggcc
     1021 cgccccgtta aatcaaaccg tctccgatcg gccgaaacgc cgacggcgaa accgatgggc
     1081 cttttccgga cggcgcccgc cgcctttcca acgtccgtcc gaattagtta aatctctttc
     1141 ccctaaaaaa cgtttcctcg ggaggagttc catgttcggg tcggtgatga cgggcgttgt
     1201 ctcgttgatg cacgtctatg ttttttggcg ggcgtgcacg gtgccgtttg tgacggcgcg
     1261 cgtgccgagg aaaggcgtgg cggcgctggg cctgctgatg tggtcggtct tttttctggg
     1321 ccgcgttctg gggcacggcg gcacgggacc ggcggccgcc gcgctggaat tcctgggcat
     1381 gaactggatg ggcgcgcttt tcctgatgac ggtgacgctg ctggcggcgg acgttctgac
     1441 cctgttcggc ctcgttttta aaaaatacgc ccccggactt cgaggcgcgg cgctgctgac
     1501 cggcctggcc ctggccgcct tcgcgctcgt ccagggactc cgccctcccg tggtgcgctc
     1561 cgacgatgtc cccctcgccg gcctcccgtc ggcgttggac ggcaaggtgc tggtggtcat
     1621 ctccgacctt cacctcggtt cacagaccgg cgcgggccgg ttggacgccg tggtggcccg
     1681 ggcggcggcg caaaaaccgg acatggttct cctgttgggc gacatcacgg agggacacga
     1741 cgcgccgcct gaaacgtttc ttttctcgtt gcggcgtctc tcggccccgt ggggcgtttg
     1801 gggcgtgctg ggcaaccacg atcctcctcg ccgcgccgcc ccggcggacg gttccctcat
     1861 ggaacaggcc ggcatccccg tgttgcggaa cgcctgggtg gaggttcaac cgggattggt
     1921 tctggcgggg cgggacggcc gctccgtgcg gcggggagga cttccggcgg aagaggccct
     1981 ggaaaaaacc ctggcgggcc ggccggccgg cgcgacgatc tttctttccc attatccgga
     2041 caaagccgag gcggcggccc gcgccggcgt tgggttgatg ctgagcggac acacccacgg
     2101 cggccaggtg tggcctttcg gttatttggt gcggcaacgt tatccccggc tgggcgggcg
     2161 ttaccggatc ggcgacatga cgctcctggt gtgccggggg acggggacct ggggtccgcg
     2221 cctgcggttg tggcatccca acgaaatact tcgtatcacc ttacgtcgaa aaaatgcaga
     2281 ataaacggga gcctcctcaa aagcgccgag cggcggggcg gcacgcgccc tcagtttcgc
     2341 cgagcgtgcc ggacgggaag ttcccgtccc aacggccgtg aaccaccgcc cctcatactg
     2401 aaagaggtcc ctttcggaaa tttcgcggaa cacgccatgg aattgcttgc ccccgtcggt
     2461 tcaaaagaat cctttatcac cgccattcgc ggcggcgccc acgccgtgta tgtgggcgtt
     2521 ccggatttca acgcccggat atcggcctcc cccctcaacc tctatgactt ggaggtgctc
     2581 atcgcgcacg cccacgagaa gaacgtgaaa gtctacctgg ccctgaaca
//
LOCUS       DAOTFD010000296         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_31708_length_2620_cov_3.692788, whole genome shotgun sequence.
ACCESSION   DAOTFD010000296 DAOTFD010000000
VERSION     DAOTFD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31708_length_2620_cov_3.692788"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="P5079_11415"
     CDS             <1..835
                     /locus_tag="P5079_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRY30629.1"
                     /translation="ACGVRTSNGSIGMDEDGRPLTDIVELAQGQGLAVGVVTTDALSG
                     ATPGAFTAHAASRRSSGIIASQQISKRITVLLGGGGEDYDLDAAKKAGYFVMTRKKDL
                     LSPAAQKKNLWLGIFSSAEIPYVDERTPDTPSLKDMTRAALGKLARNDRGFFLMVEGG
                     RIDHAAHANKTPRVFDEVLDFDAAVGAVLEFVKAHPDTTLFLTADHETGGVGLTKKEA
                     GEYYPTAEDFEKGRGLHWLSKDHTAAPVLLVGVGADAASVNGWHDNTDLFQLVKDAFG
                     L"
     gene            873..1931
                     /locus_tag="P5079_11420"
     CDS             873..1931
                     /locus_tag="P5079_11420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY30630.1"
                     /translation="MSRHPVSDASTEREWFERIARLSVDLVTIFDAERLLQKIMEIFA
                     EVGKVSKGSLMVPTSSGDRLEIRASVGLSERAREIVRPRLGEGVAGRVAAGGKPILLE
                     DTSVDDGRYLDFLDTDRRPRPKETLLSLPLVYRDQVLGVVNLDHKLTGEPFRREDMDR
                     LTVLCNFAAVAIANQRFYQESLTDGLTGLVAQKTFKIRLQEEVSRARRYSAFLSVLFL
                     DLDHFKRFNDTHGHPLGDQALRHLARLLRESTRSIDVVGRCGGEEFGILLLETDLPSA
                     RVVGERIRSRVESTPLSVEGSRYPITVSIGAAALSPSERVLTPEALLGRADDALYSAK
                     GQGRNRLVLWQENIPKKK"
     gene            2004..>2620
                     /locus_tag="P5079_11425"
     CDS             2004..>2620
                     /locus_tag="P5079_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY30631.1"
                     /translation="MGPDKTTTNIDTLCVNTIRFLAAEAVEKANSGHPGAPMEAATLG
                     YVLWTRFLKHNPRHPHWPNRDRFVLSAGHASMLLYGLLHLTGYDLTLDDLKNFRQWGS
                     RTPGHPENFLTPGVETTTGPLGQGFGNAVGMALAARFLAAKFNRPGYPVVDHFVHVFA
                     GDGDLMEGVTSEAASLAGHLRLGNLKAVYLDNRITIEGSTALTFS"
BASE COUNT          462 a          879 c          821 g          458 t
ORIGIN      
        1 ggcgtgcggg gtgcggacct ccaacgggtc catcggcatg gacgaggacg gccgcccgct
       61 gaccgacatc gtggagctgg cccagggcca gggattggcc gtcggcgtcg tcaccacgga
      121 tgccctctcc ggagccaccc cgggcgcctt cacggcgcac gccgcctccc gccgctccag
      181 cggcatcatc gcttcccagc agatttccaa acggatcacg gtgcttttgg gcggcggcgg
      241 cgaggattac gatttggacg cggcgaagaa ggccgggtat ttcgtcatga cccgcaaaaa
      301 agacctcctc tccccggcgg cccagaagaa gaacctgtgg ctgggcattt tttcgtcggc
      361 ggagatcccc tacgtggacg agcgcacgcc ggacacccct tccctgaagg acatgacccg
      421 ggccgccctg gggaaactgg cgcggaacga ccggggattt ttcctgatgg tggagggggg
      481 acggatcgac catgcggcgc acgccaacaa gacgccgcgc gtgttcgacg aggtcctcga
      541 tttcgacgcc gcggtgggcg cggttctgga attcgtcaag gcccatccgg acaccacgct
      601 gttcctcacc gccgaccacg agaccggcgg cgtcggactg accaagaaag aggccggcga
      661 atattacccc acggcggagg atttcgaaaa gggtcgcgga ctccattggt tgagcaagga
      721 ccacaccgcc gcccccgtgt tgctggtggg cgtgggggcc gacgccgctt ccgtcaacgg
      781 ctggcacgac aacaccgatt tgttccaact cgtcaaggac gccttcggtc tctgacgggt
      841 gtttttttga acaaaaggag ccgcttcccc gcatgagccg gcatcccgtc tccgacgcct
      901 ccaccgagcg cgagtggttc gaacgcatcg cccgcctttc cgttgacctc gtcaccatct
      961 tcgacgccga acggctcctc cagaagatca tggagatttt cgcggaggtg gggaaagtct
     1021 ccaaggggtc cctgatggtg ccgacctcct ccggcgaccg gctcgagatc cgggcttccg
     1081 tcggcctttc cgaacgggcg cgggagatcg tccggccgcg cctcggcgaa ggggtggccg
     1141 gccgggtggc ggccggcggc aaaccgatcc ttctggagga cacctccgtc gacgacggac
     1201 gatacctgga ttttttggac accgaccgcc gcccgcgccc gaaagaaacg ctgttgtccc
     1261 tgccgctcgt ctatcgtgac caggtcctcg gggtggtcaa cctggaccac aagctcaccg
     1321 gggaaccgtt ccgccgggag gacatggacc ggctcaccgt cctctgtaac ttcgcggcgg
     1381 tggccatcgc caaccagcgg ttctaccagg aatccctcac cgacgggctc accgggctcg
     1441 tcgcccagaa aacgttcaag atccgcctgc aggaagaggt ctcccgcgcc cgccgctact
     1501 cggcgttcct ttccgtcctt tttctggatc tggaccactt caaacggttc aacgacaccc
     1561 acggccaccc gctgggcgac caggccctga gacacctggc ccggctgttg cgggaaagca
     1621 cgcgatccat cgacgtcgtc ggccgctgcg ggggggagga gttcggcatc ctgctgttgg
     1681 agaccgacct gccttcggcg cgcgtggtgg gcgaacggat ccggtcccgg gtggagtcca
     1741 cgccgttgtc ggtggagggg agccgctatc cgatcaccgt ttccatcggc gccgcggcgt
     1801 tgtctccgtc ggaacgcgtc ctcacgcccg aggccctcct gggccgggcc gacgacgccc
     1861 tctacagcgc caagggccag ggccgtaacc ggctcgtcct ttggcaggaa aacatcccga
     1921 agaaaaaata attctcccaa cagcgggccg ttggaaaacg gcggatggga aaagggttct
     1981 ttagtttgta aaatagaacg atcatgggcc cggataaaac aacgacgaac atcgacaccc
     2041 tttgcgtcaa caccatccgc tttctggccg cggaggccgt tgaaaaggcc aactccggcc
     2101 atccgggcgc ccccatggaa gccgccacgc tcggctatgt gctgtggacc cgcttcctca
     2161 aacacaatcc ccgccacccg cactggccca accgcgaccg ctttgtcctc tccgccggcc
     2221 atgcgtccat gctgctctac ggtctcctcc atctcacggg ttacgacctg accctggacg
     2281 atctgaaaaa tttccgccag tggggcagcc gaactcccgg ccatccggag aacttcctga
     2341 cgccgggggt ggagaccacc acggggccgc tggggcaggg gttcggcaac gccgtgggca
     2401 tggccctggc cgcccggttt ttggcggcca aattcaatcg gcccggttat ccggtcgtgg
     2461 accacttcgt gcacgttttt gccggcgacg gggacctcat ggagggcgtc acctcggagg
     2521 ccgcgtcgtt ggccggtcat ctgaggttgg ggaacctcaa ggcggtgtat ctggacaacc
     2581 gcatcaccat cgaaggttcc accgcgttga cgttttcgga
//
LOCUS       DAOTFD010000297         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_31792_length_2616_cov_4.292073, whole genome shotgun sequence.
ACCESSION   DAOTFD010000297 DAOTFD010000000
VERSION     DAOTFD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31792_length_2616_cov_4.292073"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1600)
                     /locus_tag="P5079_11430"
     CDS             complement(191..1600)
                     /locus_tag="P5079_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY30632.1"
                     /translation="MKKSGEVVVIVGAGPAGLGAAWRLQQGGHDNFVVLEANSHPGGL
                     SASFVDEKGFTWDLGGHVQFSHYPEYDSVLRKALGNDWLYHQRESWIWIHRRFVPYPF
                     QYNFHLLGHRIADAVLGDLRHLAASSPRRPASNFQEWIRRTFGRALGALFMVPYNQKV
                     WGVPLHRMSARWVGERVAVPDVERVARNVESGRTDITWGPNNLFQFPRQGGTGAIWSA
                     VSRFIRSGRIQYGESVRRVFLKEHAVETSSGRRWRYDHLFNTMPLDIFCDISPDLAPR
                     PRRAARRLQHGAVHVVGVGLRGGRPDSLRTKNWMYFPEPNSPYYRVTVFSNYSPRNVP
                     PGPGHWSLMAEVTETRFKKVDSKTLVRDVLRALRQDRLIPHSRHIVDIWRRRVEHAYP
                     TPTLERDELLGAILPSLEARSVFSRGRFGGWKYEAGNQDHCFMQGVEWAERFLYGKKE
                     TTYDVPERINARPPRKHLK"
     gene            complement(1612..2325)
                     /locus_tag="P5079_11435"
     CDS             complement(1612..2325)
                     /locus_tag="P5079_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30633.1"
                     /translation="MAGAADRPVLSVVIPFFNEERAIERVVEQWTATLNGLGVAYEFR
                     AYDDCSRDGSWAVLSGLTGRFPRLVLSRNAANLGHGPTIHRGYRESRGDWVFQTDGDN
                     EIAPDHFRALWDGREGKDIVIGRRRFEKRPWPRRLISWVSRSLIKIWTGRTLDVNVPY
                     RLVKKQKLDEALACIPERTFAPNVAMSALACKRGWAISEIDVVSRERSTGQVSIRKWG
                     LWKAALKSFLQTAVILVRS"
     gene            complement(2358..>2616)
                     /locus_tag="P5079_11440"
     CDS             complement(2358..>2616)
                     /locus_tag="P5079_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRY30634.1"
                     /translation="EKLGRSIAWLDTGTHEALLQAANFIQVIEDRQGLKISCVEEIAY
                     KLGYITADELRRLAEPMKKNEYGLYLLRMLNELGEGDVGTR"
BASE COUNT          491 a          873 c          780 g          472 t
ORIGIN      
        1 cagacgcggg cggaatgctg ccagccctca tcccccgcca ccgatcccac gcgctgaagg
       61 gcgtcgtatg gcgcgtcgga ggtcaaatga accagtcgga gcccggcgga cagggccaac
      121 agcgtccccg ccaacaatcc gaagagcgcc agccgctttt ttccgcccat cgtttccgcc
      181 gaagaggcgt tcatttcaaa tgtttccgcg gcggccgcgc gttgatccgc tcggggacgt
      241 cgtaggtcgt ttcctttttc ccatacagaa acctctccgc ccattcgacg ccctgcatga
      301 aacaatggtc ctgattgccc gcctcatatt tccaaccgcc gaaacgtcca cgggaaaaga
      361 ccgatcgagc ctccagcgaa gggaggatcg cccccaacag ctcatcgcgt tccaacgtgg
      421 gcgtggggta ggcatgttcg acccggcggc gccagatgtc cacgatgtgc cgggaatgcg
      481 gaatgagccg gtcctgccgc aacgcccgca gcacgtcgcg gacgagcgtc ttcgagtcca
      541 cttttttgaa ccgagtctcc gtcacctccg ccatgaggga ccaatgcccc ggccccggcg
      601 gcacattccg cggcgaataa ttggaaaaaa cggtcacgcg atagtaggga ctgttcggtt
      661 ccgggaaata catccagttt ttcgtccgaa gcgaatcggg acgacctccc ctcagaccca
      721 cgcccaccac atggaccgcg ccgtgctgca accggcgggc cgcccgccgc ggccgaggag
      781 ccaggtccgg ggagatgtcg caaaagatat ccaacggcat ggtgttgaag aggtggtcgt
      841 accgccaacg ccggccggac gaggtctcca ccgcgtgttc tttgagaaaa acccgccgca
      901 ccgactcccc atactggatg cggccggacc ggatgaagcg actgacagcg ctccaaatgg
      961 cgcccgtccc tccctgccgg gggaattgaa agaggttgtt gggcccccag gtgatgtccg
     1021 tccggcccga ctcgacgttc cgcgccaccc gctccacatc cggcaccgcc acccgctccc
     1081 ccacccaacg ggcgctcatc cgatgaagcg gcacgcccca aaccttttgg ttgtagggca
     1141 ccatgaagag cgcgccgagg gctcttccga aggtccttcg tatccattcc tgaaaattcg
     1201 aggcgggacg gcgcggggag gaggcggcaa ggtgtcggag gtcgcccaga acggcgtctg
     1261 cgatccgatg acccaacaaa tggaaattat attggaaagg gtaggggaca aaccggcggt
     1321 ggatccatat ccaggattcc cgctgatggt aaagccagtc gttccccaag gccttgcgga
     1381 ggacgctgtc gtattccggg taatggctga actggacgtg gccgcccagg tcccacgtaa
     1441 aacctttttc gtcgacgaaa ctggcggaaa ggccgccggg atgggagttc gcctccaaca
     1501 ccacgaaatt gtcgtgtccg ccttgctgaa gccgccaggc ggcccccagc cccgccggcc
     1561 cggcgccgac gatgacgacg acctccccgc tctttttcat ccgtcgccct ttcacgaccg
     1621 gacgaggatg acggccgttt gcaggaaaga tttcagggcc gccttccaaa gcccccattt
     1681 ccgaatggag acctgtcccg tgctccgttc ccggctgaca acgtcgatct cggaaatggc
     1741 ccagcccctt ttacaggcca gggcgctcat ggccacgttg ggagcgaacg tccgttccgg
     1801 tatgcaggcc agggcctcgt cgagtttttg ttttttcacc agccggtagg gcacgttcac
     1861 gtccagggtg cggccggtcc aaatttttat gagcgaccgc gagacccagc tgatcaaccg
     1921 gcggggccaa ggcctttttt cgaaacgccg ccggccgatc acgatgtcct tcccctcacg
     1981 gccgtcccag agggcccgaa aatgatccgg ggcgatctcg ttgtcgccgt cggtctgaaa
     2041 gacccagtcg ccccgggatt cccggtatcc ccggtggatc gtcgggccgt gccccaggtt
     2101 cgccgcattg cgtgagagga cgaggcgggg gaaacgcccc gtcaggccgg agaggacggc
     2161 ccaactgccg tcgcgggaac aatcatcgta agcgcggaac tcataggcga cgccgagccc
     2221 gttcagcgtg gccgtccact gttcgacgac ccgctcgatg gcgcgttctt cgttgaagaa
     2281 cgggatgacg acgctcaaca ccggccggtc ggccgcgccg gccatttatt tcagcccctt
     2341 cgccgcccta tcgtccatca gcgcgtcccg acgtcccctt ctcccagttc gttcagcatc
     2401 ctcaacaaat agaggccgta ctcgttcttc ttcatgggtt ccgccaggcg gcgaagttca
     2461 tcggcggtga tatacccgag cttgtaggcg atctcctcca cgcaggagat cttgagcccc
     2521 tgccggtcct cgatgacctg gatgaagttg gcggcctgga ggagcgcctc gtgcgtgccg
     2581 gtgtcgagcc aggcgatgct gcggccgagt ttctcc
//
LOCUS       DAOTFD010000298         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_32173_length_2597_cov_3.806452, whole genome shotgun sequence.
ACCESSION   DAOTFD010000298 DAOTFD010000000
VERSION     DAOTFD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32173_length_2597_cov_3.806452"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="P5079_11445"
     CDS             <1..945
                     /locus_tag="P5079_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY30635.1"
                     /translation="ILVTGAAGFIGSWVCKSLRDRGDNVIGLDDFNDYYDVSLKRARV
                     RRFIPGVKVYKADVADRRALERIFHRHRFRQVCHLAAQAGVRYSLQNPFAYESANVLG
                     SLNLLEMCRRFGVRSFIYASSSSVYGGNKKVPFSVRDRVDRPLSLYAATKRAGELMAH
                     VYHRLYGISCTGLRFFTVYGPWGRPDMALFKFTRAILRGRPIDVYNYGRMERDFTYVT
                     DIAAGVLAALDKNYPFEIFNLGNSRPVRLRRFIGCLEKELGRRARMRLLPLQPGDVPR
                     TCADLRETRRKLGYRPRVPVEKGIREFVRWYKDYYRPA"
     gene            1317..>2597
                     /locus_tag="P5079_11450"
     CDS             1317..>2597
                     /locus_tag="P5079_11450"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRY30636.1"
                     /translation="MKPEEILKTVRDKNIKFIRLWFTDILGQLKSFAITPHELEGALK
                     DGMGFDGSSIEGFARIYESDLMARPIPETFQLLPWRPKEDGVARMFCDVITPEGKPFP
                     GDSRYVLKRALSEAEKLGLKLYVGPELEFFYFKSDQAAECIDQGGYFDAVPLDEAHDL
                     RRQTIIYLEQMGVQVEYSHHEVAPSQHEIDLRYNDALTMADRVITYKVAVKQVAHEQN
                     VYASFMPKPIAGINGSGMHVHQSLFKKGQNAFFSAKDPYYLSDIAKHYIAGILRHARE
                     ICIITNQWVNSYKRLVPGYEAPVYICWGRRNRSALVRVPEYKPGRSEATRIEVRFPDP
                     SCNPYLAFAVMLMAGLAGIKGKYKLPESVEADVYEMNELERRAQRIETLPGSLIEALE
                     CAEQSALMKHALGEHVFDKLLLNKRLEWDAYRTHV"
BASE COUNT          518 a          759 c          802 g          518 t
ORIGIN      
        1 attctcgtca ccggcgccgc cggattcatc ggttcctggg tctgtaaatc gctccgggac
       61 cgcggcgaca acgtcatcgg tctcgacgat ttcaacgatt attacgacgt ttccctcaag
      121 cgggcgcgcg tgcgccggtt catccccggc gtcaaggtct ataaggccga cgtcgccgac
      181 cgccgcgcgc tggaacgcat tttccatcgg catcggttcc gtcaggtgtg tcacttggcg
      241 gcccaggcgg gggtgcgcta ttccctgcag aacccgttcg cctacgaatc ggccaacgtg
      301 ctcggctcgt tgaacctgtt ggagatgtgc cggcgtttcg gcgttcggtc gttcatctac
      361 gcctcctcct cctcggtcta cggcggcaat aaaaaggtgc ccttttccgt gcgcgatcgg
      421 gtggaccggc cgctctccct ctacgccgcc accaagaggg ccggcgaatt gatggcccat
      481 gtctatcatc ggctttacgg catttcctgc acgggccttc ggtttttcac ggtctacggc
      541 ccgtgggggc ggccggacat ggctctcttt aaattcaccc gcgccatcct ccggggccgc
      601 cccatcgatg tctataacta cggccggatg gagcgggatt tcacctacgt cacggacatc
      661 gcggccggcg tgctggccgc actcgacaag aattacccct ttgaaatatt caacctgggg
      721 aattcccgcc ccgtgcggct gcgccgtttc atcggctgtc tcgaaaagga actcggccgc
      781 cgggcccgga tgagactcct gccgttgcag cccggcgacg tgccccggac gtgcgcggac
      841 ctccgcgaga cgaggcggaa attggggtat cggccgcggg tcccggtgga gaaggggatt
      901 cgggagttcg tccggtggta taaggactac tatcggccgg cgtgaccgcc ggacattgtc
      961 cgccgacggg atgacaaatt tcgaaaaaag gggtagcatt taagagttcc cccgggtgcg
     1021 ttcgggggac gtcacgattt tcgggccggc tatcgctcga aaaccactcg gaacaggggg
     1081 ccgggttaat gcattcacaa ggaggctgcc atgggtggag gagaagcagg agcgggtcgt
     1141 tgtgaacgtt gcggtcagga agggcctgtt cgtaaggtgg gggggatgat gtatttgtgt
     1201 gcccgctgcc ggcatgtcta tgaaatcgag gatgcccgcc gccgcgcggc gctgaacaag
     1261 gacaatcctc agggcaaaac ggaaaagaag taagacatcg ggaaagggga ttttcgatga
     1321 aaccggaaga gatactgaaa accgtccgag acaaaaacat caagttcatc cgtctgtggt
     1381 tcacggatat cttggggcaa ttgaaatcgt tcgccatcac gccgcatgaa ctggaggggg
     1441 cgctcaaaga cggcatgggc ttcgacggtt cgtccatcga ggggttcgcc cgcatctatg
     1501 aatccgacct gatggcccgg cccatcccgg agacatttca actccttcct tggcgtccca
     1561 aggaggacgg cgtggcgcgg atgttttgcg acgtcatcac gccggaaggg aaacccttcc
     1621 cgggagattc ccgctatgtc ctcaagcggg ccctgtcgga ggcggagaag ctggggttga
     1681 aactctacgt cggaccggaa ctggagtttt tctacttcaa gtccgaccaa gcggcggaat
     1741 gcatcgacca ggggggctat ttcgacgccg tcccgctcga cgaggcgcac gacctgcgcc
     1801 gtcagacgat catctatctg gaacagatgg gcgtgcaggt cgaatattcc caccacgaag
     1861 tggccccgag ccagcacgag atcgacctgc gctacaacga cgccctcacc atggccgacc
     1921 gggtcattac ctacaaggtg gcggtgaaac aggtcgctca cgaacagaac gtctacgcct
     1981 cgtttatgcc caaacccatc gccggcatca acggcagcgg catgcacgtg catcaatccc
     2041 tgttcaagaa gggccagaac gcttttttct cggccaaaga cccttactat ctctccgaca
     2101 tcgccaaaca ctacatcgcc ggcatcctcc ggcatgcacg ggagatatgc atcatcacca
     2161 atcagtgggt caactcctac aaacgcttgg tgccgggata cgaagcgccc gtgtatatct
     2221 gctggggccg ccggaaccgg tccgcgctgg tgcgggtgcc ggaatacaag ccgggccggt
     2281 cggaagccac gcgcatcgag gtgcggtttc cggatccgtc ctgtaacccg tatctggcct
     2341 ttgccgtgat gttgatggcg gggctggcgg ggatcaaggg caaatacaaa ctgccggaat
     2401 ccgttgaggc ggatgtgtat gagatgaacg aattggagcg tcgcgcccaa cgcatcgaga
     2461 cgctgcccgg ttcgttgatc gaggcgttgg aatgcgccga gcagtcggcg ttgatgaaac
     2521 acgctctcgg agagcatgtc ttcgataagc ttcttctgaa taagaggttg gagtgggatg
     2581 cctaccggac ccacgtt
//
LOCUS       DAOTFD010000299         2566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_32837_length_2566_cov_3.917164, whole genome shotgun sequence.
ACCESSION   DAOTFD010000299 DAOTFD010000000
VERSION     DAOTFD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32837_length_2566_cov_3.917164"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5079_11455"
     CDS             <1..292
                     /locus_tag="P5079_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30637.1"
                     /translation="PRIGRSKIRPVRDSLRTFQFLVEIIVYFNPLKAVMPLIVALFLG
                     ALLCGTAYVYLKNDSLLISSVLFFSASCVVFAVGMLMNQLRLTRNSQNKNPC"
     gene            286..1851
                     /locus_tag="P5079_11460"
     CDS             286..1851
                     /locus_tag="P5079_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY30638.1"
                     /translation="MLTALGLIFLAAANLWACGPVFSWAYFSQESMNLAALMDPLFKF
                     SLNAMNEFESYGVWRPVGGLIWWLKYLVAGDTFWGQHLTEYLGYSLAAFGVYLLLRQT
                     TKSSFLAAVIAVTCWFLPARISCIAYFITGTTWMLLLGFFALYWGVKGVEERSWKTIT
                     LSCVSFASALLTREYSVIFWAVLVGYYVIVEPSSFRHRRKSVYALLLSWSVLLTWFLV
                     MRLAVFGDRIPYPLVNSTLSPHRFLLCFSHIPPLAVKSFAGGDSLRLILSSAKTALPF
                     GFYSLLAAIGAFLFFRRQTDAPPTEKRSFWFRLALWGGVCCLFAGLPFAYLRDIVETP
                     SYFFITPKDGTEHHRIILVFFFSLWTLFFIARAISARVTLILGVLVTTLLFQLDASYF
                     RFPEGSTYASWGEEGTAVPTFEKNIKDLNLPPGAKIVYWDSTVSSVPLLTHMLSLRLL
                     RLSMPHRWRLESWGTLFQRDSAPLPCNRPVDPGVALYVWSHVSPDEPNLIRVMGYLPA
                     DDRTMTLMKFRLP"
     gene            1848..>2566
                     /locus_tag="P5079_11465"
     CDS             1848..>2566
                     /locus_tag="P5079_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRY30639.1"
                     /translation="MKKSLSRAGLVAFGLFLFPVGPELLLRTMAHLYRKTHIAIPATE
                     HPDHKILCLGDSFTFGVGARLGQDYPHQLQDILSSRGHRVNVINQGIPNQRSGNVART
                     APRLLDRYEPDIVLLLIGFNDPGYLGPKDYEPGRYKRYDMNATVGLVDRLSVAAGRSR
                     LFRAAQHLQRRWRDSHPRTSAGPVPVARKLPAPVHTLDKITQAYVHLYDRQEYADALR
                     LFTEAWAVEKTEEALYGALETT"
BASE COUNT          425 a          881 c          621 g          639 t
ORIGIN      
        1 tccccgcatc ggccgctcca aaatacggcc ggtccgggat tccctgagga cgtttcaatt
       61 cctcgtcgaa atcatcgtct atttcaaccc cctgaaagcc gtcatgcctt tgatcgtcgc
      121 ccttttcctc ggggcccttc tctgtggaac cgcctatgtt tatctgaaga acgactccct
      181 tttgatttcc tccgtcctct ttttttccgc ctcctgcgtg gttttcgccg tcggcatgct
      241 tatgaaccaa ctccgcctca cccgcaactc ccagaacaaa aacccttgct gacggcgctg
      301 ggactcatct tcctggctgc cgccaatctg tgggcctgcg gaccggtgtt ttcatgggcc
      361 tatttttccc aggaatccat gaacttagcc gccctgatgg accccctttt caaattctcc
      421 ctcaacgcca tgaacgagtt tgagtcttac ggcgtctggc ggcccgtcgg cggattgatt
      481 tggtggctga aatacctggt ggccggagat acgttctggg ggcaacacct gacagaatac
      541 ctcggctatt ccctggccgc cttcggggtc tatcttcttc tccgccagac cacaaaatcc
      601 tcttttctgg cggccgtcat cgccgtcacg tgctggttcc tccccgcccg catctcctgc
      661 atcgcctatt tcatcacggg aacgacctgg atgctcctgt tgggtttctt cgcgctgtat
      721 tggggagtca aaggggtgga ggaacgctcc tggaaaacaa tcaccctgtc gtgcgtttcc
      781 ttcgcctcgg ccctcctcac acgggaatat tcggtcatct tctgggccgt attggtcggc
      841 tactacgtca tcgtcgagcc ttcttcattc cgacaccggc gaaaatccgt ttatgccctg
      901 cttctctcct ggtcggtcct tctcacctgg tttctcgtca tgcgtttggc cgtgttcggc
      961 gaccggatcc cgtatcccct ggtgaactcg acgctctctc cgcatcgttt cctcctctgt
     1021 ttttcccaca tccccccgct ggccgtcaaa tccttcgccg ggggggacag cctccgcctc
     1081 atcctgtcgt cggccaagac cgccttgcct ttcggttttt attcgctcct ggccgcgatc
     1141 ggcgctttcc tttttttccg acggcaaacg gacgcccctc ccactgaaaa aagatctttc
     1201 tggttccggt tggccctctg gggcggggtc tgttgccttt ttgccggcct ccccttcgcc
     1261 taccttcggg acatcgttga gacgccgtct tactttttca tcacccccaa agacggcacc
     1321 gaacatcacc gcatcattct ggtgttcttt ttctcgcttt ggaccctttt cttcatcgcg
     1381 cgcgccatct ccgcccgcgt cacgctgatt ctcggcgtcc ttgtcacaac gcttcttttt
     1441 caattggatg cctcctactt ccgtttcccg gaaggctcca cgtacgcgtc ctggggagaa
     1501 gaaggcaccg ccgtccccac ttttgagaaa aatataaagg acctgaacct gccccccggc
     1561 gcgaaaatcg tctactggga ctccaccgtc tcctccgtgc ccctcctgac ccacatgctt
     1621 tccctgcggc tcctccgtct ctccatgccc caccgctggc gactggaaag ctggggaacc
     1681 ctctttcagc gcgattccgc cccgcttccc tgcaaccggc cggtggatcc cggcgttgcc
     1741 ctctatgttt ggtcccacgt ctccccggac gaaccgaatc tcatccgcgt gatggggtat
     1801 ctgccggccg atgaccgaac aatgaccctg atgaaatttc gtcttccatg aaaaaatccc
     1861 taagccgggc gggactcgtc gccttcgggc tgttcctctt tcccgtcggt ccggaacttc
     1921 tccttcggac gatggcccat ctctatcgga aaacgcatat cgcaatcccg gccaccgaac
     1981 atcccgacca taaaattctc tgtctggggg attcctttac cttcggggtc ggcgcccgat
     2041 tggggcagga ctacccccat caattacagg atattctttc ctcccgcggc catcgggtga
     2101 atgtcatcaa ccagggaatc cccaaccaac gttccggcaa cgtggcccgg accgcgccac
     2161 gactgcttga ccgatacgag ccggacatcg tcctcctcct catcgggttc aacgaccccg
     2221 gttatttggg accgaaagac tatgagccgg ggagatacaa gcgttacgac atgaacgcga
     2281 ccgtcgggct cgtcgaccgt ctttctgtcg ccgcgggacg ttcacggctt ttccgagcgg
     2341 ctcaacatct tcaacggcgc tggcgtgatt cccatccccg gacttcggcc ggtcctgtcc
     2401 cggtggcgag gaaattgccc gcgcccgtcc acacgctgga caaaatcacg caggcctacg
     2461 tccaccttta cgaccggcag gagtacgccg acgctctccg gcttttcacc gaggcctggg
     2521 ccgtcgaaaa aacggaggag gcgctttacg gcgcgctgga aacgac
//
LOCUS       DAOTFD010000300         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_32862_length_2565_cov_3.154183, whole genome shotgun sequence.
ACCESSION   DAOTFD010000300 DAOTFD010000000
VERSION     DAOTFD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32862_length_2565_cov_3.154183"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2565
                     /locus_tag="P5079_11470"
     CDS             <1..>2565
                     /locus_tag="P5079_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30640.1"
                     /translation="EAVTDLAAGVGMEDGGIVLDWTSTGNDGASGALLAGSSFYIQYS
                     TFDLTWSTMDAQVALATSNVNAGELQRCVLPALNPGSTYYLRLWTSDESGNCSALSNG
                     TTNWAQDLALSSPTAVSVGTVEGGQVGLSWTVPPAPAHVDDRALYRVYRATQDFSGVA
                     DAGVLLSSEASHPAAAATVTGLWGETTYYFGVTAVDQGDQENGLMSLALEGALSTVVS
                     TRTADVAPGAVSDLTALPGSADGEIRLSWTSPGDDGEAGDLSAGIFLIKFSSVQIINA
                     ADFDAPPFAVTSLAVSTNTAALSPATLLVTGLTAGCSYWFAVKTRDEAAGNYSLWNSS
                     GDQAGVNPLAYSAAMDAPPAAPSGLTASSSPAQVDLSWTAGAEPDLSAYEIYQSSWSE
                     AEGFSLLDTVLSSQTDYQKTGLVNGNTYYYKVRAQDQTGHFSDYSSTVSAVPGVALGA
                     APVFSTSTVVVSSSVLHFVWSLTANADGYRVIQSSSGFSLSGDLGPSASAYSLTGLPV
                     NSSHTVYVRAFSGLQTLDSGTTTHYTFAAVPAGLASPAQTVSSLDLDWVDGGNPPGTR
                     YDVERSSTGVDGWTLRAEKITATTHYDSGLAEYATYYYRVYAVNGAEVRTAAFVGLTT
                     MTAGISPAAVSNLAANPGAADGAVELVWMAPGDDAMSGLASSYVVKYATFDFAAAEFD
                     AGYIHTFTQSWVPKTPGLTEGLSPLREVTGLYPGTTYYFAIKAVDEAGLLGTWSTSGV
                     NLERFGYAQDNVPDSPDGLSASAGNAQITLSWTALSETDLDHYRIYMDSMAPLGDAYT
                     LLGTTPAAFAAYVANGLSNETTYSFKVSGVDQGSSGDFFSHALEGPLSLESSTATW"
BASE COUNT          388 a          807 c          886 g          484 t
ORIGIN      
        1 ccgaggcggt gacggacttg gcggccggtg tcgggatgga ggacggcggg atcgtcctgg
       61 actggacgtc gaccgggaac gacggtgcct ccggcgctct gttggcgggg agttccttct
      121 acatccaata ctcgacgttc gacctgacct ggagcacgat ggatgcgcag gtggccttgg
      181 cgacgtccaa cgtgaacgcg ggggagctgc agagatgcgt cctgccggcg ctgaaccccg
      241 gttccacgta ttaccttcgt ttgtggacgt cggacgaatc cggaaactgt tccgccctct
      301 ccaacggcac cacgaactgg gcgcaggatc tggcgctttc gtcgccgacg gcggtgtcgg
      361 tggggacggt ggaggggggg caggtcgggt tgtcgtggac cgttcccccg gcgccggccc
      421 atgtggacga ccgggccctt taccgggtct atcgcgccac ccaggacttc tccggcgtgg
      481 cggacgccgg ggtgctcctg tcgtccgagg cgtcccatcc cgccgcggcg gccacggtga
      541 cgggcttgtg gggcgagacg acgtattatt tcggcgtgac ggcggtggac caaggggatc
      601 aagaaaacgg gttgatgagc ctcgccctgg agggcgcgct ctcgacggtg gtgagcaccc
      661 ggacggcgga tgtggctccc ggcgccgtgt cggacctgac ggctctgccg ggttcggcgg
      721 atggcgagat ccggttgagc tggacctcgc ccggcgatga cggcgaggcc ggcgatctct
      781 cggcgggaat tttcctgatc aagttcagca gcgtccagat catcaatgcg gcggatttcg
      841 atgctccgcc gtttgccgtc acctccctcg ccgtgtcgac gaacaccgcc gccctttcgc
      901 ccgccacgtt gttggtgacc gggttgaccg cggggtgcag ttactggttc gccgtcaaaa
      961 cgcgggatga ggcggcgggc aattattcgt tgtggaattc gagcggcgat caggcgggcg
     1021 tcaatccctt ggcctattcc gcggccatgg acgcgccccc cgccgccccc tcgggactga
     1081 cggcctcctc gtcccccgcc caggtcgatt tgtcgtggac ggccggcgcg gaaccggacc
     1141 tgtcggcgta tgagatctac cagtcctcgt ggtccgaggc ggagggtttt tctctgttgg
     1201 acacggtcct ttcctcccag acggattacc agaagaccgg tctcgtcaac ggcaacacgt
     1261 attactacaa ggtccgcgcc caggaccaaa cgggtcattt cagcgattat tcctccacgg
     1321 tcagcgccgt gccgggagtg gcgctggggg cggccccggt cttcagcacc tcgacggtgg
     1381 tggtgagctc cagcgtcctc catttcgtct ggtcgttgac cgccaacgcg gacggctacc
     1441 gggtgatcca gagcagttct ggtttttcgc tgtccgggga cttggggccg tcggcgtcgg
     1501 cctactccct caccggcctg ccggtcaaca gttctcacac ggtgtacgtg cgggcgttca
     1561 gcggcttgca gacgctcgat tccggcacga ccacccacta caccttcgcc gccgtgccgg
     1621 ccggcctggc cagtcccgcg cagaccgtgt cgtcgttgga cctggattgg gtcgacgggg
     1681 ggaatccccc ggggacgcgc tacgacgtgg aacggtcctc cacgggcgtc gacggctgga
     1741 ccctgcgcgc ggagaagatc accgcgacga cgcattacga ttccgggttg gccgaatacg
     1801 cgacctacta ctatcgcgtc tacgccgtca acggtgccga ggtgcggacc gcggcgttcg
     1861 tcggtttgac gaccatgacg gccgggatct cgccggcggc ggtgtcgaac ctggccgcca
     1921 atccgggggc ggccgacggc gcggtggagc tcgtctggat ggctcccggc gacgatgcga
     1981 tgtccggcct ggcctcgtcc tatgtggtga aatacgcgac cttcgatttc gccgcggcgg
     2041 agttcgacgc gggctatatc cacacgttca cgcagagctg ggtccccaag acgccgggtt
     2101 tgaccgaagg gctgagccct ctccgtgagg tgacgggcct ctatcccgga acgacctatt
     2161 acttcgcgat caaagccgtg gacgaagccg gcctcctggg gacgtggtcc accagcgggg
     2221 tgaatttgga aaggttcgga tacgcgcagg acaacgtgcc ggattctccc gacgggttga
     2281 gcgcgtcggc cgggaacgcg cagatcacgc tctcgtggac ggcgctttcc gagacggatc
     2341 tggaccatta ccggatttac atggattcca tggcgccttt gggcgacgcc tacacgctgc
     2401 tggggaccac cccggcggcg ttcgccgcgt acgtggcgaa cggtttgagc aacgagacga
     2461 cctattcgtt caaggtcagc ggcgtcgacc aggggagttc gggcgatttc ttcagccacg
     2521 cgctggaggg cccgttgtct ctggaaagtt cgacggccac gtggg
//
LOCUS       DAOTFD010000301         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_33289_length_2544_cov_3.327039, whole genome shotgun sequence.
ACCESSION   DAOTFD010000301 DAOTFD010000000
VERSION     DAOTFD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33289_length_2544_cov_3.327039"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2544)
                     /locus_tag="P5079_11475"
     CDS             complement(<1..>2544)
                     /locus_tag="P5079_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30641.1"
                     /translation="NNGIFEPAIDITSVSRLSQGTEKFTSNAATITLTTPEVIYSSAA
                     GQYFFVTYDISNLAQVGRTLGLSIGTSHYVTVGVPDRVNYVLTSDSDFGTITEAADNV
                     VLGVRDLAGDLVTGNGVKQGSVNIPFLQFSLQTDVSQAEFQAIRVERIGSSNVNPLLP
                     EGSNSDVAAIRVWDDANFNLKFDAGDTLISVGTNTFNQAVEADKQKEIAITTSPAVVI
                     GKGAPRVFFLTYDISNSAAATNTVGVKVVDQNAVTMSAPNGMVGYYQDLVTKTTYLYS
                     FEGSRVTINPILIQISAESMVPAAVSQLTQNVPVLKMKMHTDVNYAILNAINLQQIGT
                     VESPLTIGQGQGDFSRAAIWLDNGDDIFVAAQDTFLGAAVHGTADFTGGVARVPLGTG
                     FTLPITTTTFFVTADIGETDVTGGQTVNHTAGFSLTGLSQITRVPSTSQDYPSNEYPL
                     RSGVLSILKFGLPRVPFITLLPKIWADYDRDYYPDVDTNGNGRLDESEKTSNPSYPRD
                     AEGRPIVTVPGIGVTASDLDGDGRTNDLDIDGDGMPDLDIDGDGLIEFDINSDGKADN
                     ILQDLNGDGVPEVDLSRDGTVNWGILPEKWTNETTKLFCRWPSISATVTEYQVGVGER
                     PDSKNAAVFNSETFNLSPNDGWISAGARNYFTVTNIGMSAARVTRTVTASVGRLLEPP
                     FTLFVQNADGFDGEGEIYIGSEIMRYSTRDQGSFVIVARGLYSTEEQDHPIGQRVTNN
                     GFFYNVRAMGETNEGPGAPVAVYRVDSTAPSAPTAPISEPEQTKKPAELGKYEIKWDP
                     SSDAESGVREYEIQERQDNDPVWRTVDYVPANRVSYIIGEGKVAGDPARP"
BASE COUNT          378 a          761 c          915 g          490 t
ORIGIN      
        1 gggccgcgcc gggtccccgg ccaccttgcc ctcgccgatg atataggaca cccggttggc
       61 cggcacgtag tccacggtgc gccagacggg gtcgttgtcc tggcgttcct ggatctcgta
      121 ttcccgcacg ccggactccg cgtcggagga cgggtcccac ttgatctcgt atttccccag
      181 ctcggcgggc ttcttggtct gttccggctc ggagatgggg gcggtcggcg cgctgggggc
      241 ggtggagtcc acgcgataga cggccaccgg cgcgccgggg ccctcgttgg tctcgcccat
      301 ggcgcggacg ttgtagaaga aaccgttgtt ggtgacgcgc tggccgatgg gatggtcctg
      361 ctcctccgtg ctgtagagcc cgcgggccac gatgacgaag gacccctggt cgcgcgtgct
      421 gtagcgcatg atctcggagc cgatgtagat ctccccttcg ccgtcgaagc cgtcggcgtt
      481 ctgcacgaag agagtgaacg gcggttccag gagccgaccg acggaggcgg tgacggtgcg
      541 ggtcacccgc gccgcggaca tgccgatgtt ggtgacggtg aaatagttgc gcgcgccggc
      601 cgagatccag ccgtcgttgg gcgagaggtt gaaggtctcg ctgttgaaca ccgcggcgtt
      661 tttgctgtcc ggacgctcgc ccacgcccac ctggtattcg gtgaccgtgg cggagatgga
      721 cggccagcgg cagaagagct tggtggtctc gttggtccac ttctccggca aaatgcccca
      781 gttcacggtg ccgtcacggg acaggtccac ctccggcacg ccgtcgccgt tgaggtcctg
      841 caggatgttg tccgccttgc cgtcggagtt gatgtcgaac tcaatgagcc cgtcgccgtc
      901 gatgtccagg tcgggcatgc cgtcgccgtc gatgtccagg tcgttggtgc ggccgtcgcc
      961 gtccaggtcc gaggcggtca cgccgatgcc gggcacggtg acgatgggcc gcccttcggc
     1021 gtcccgcggg taagacgggt tgctggtctt ttccgattcg tccagccggc cgttgccgtt
     1081 ggtgtccacg tccggatagt aatcccggtc gtagtcggcc caaatcttcg gcagcagcgt
     1141 gatgaagggc acgcggggca ggccgaattt caggatgctc agcacgcccg accgcagggg
     1201 atattcgttg ctcggatagt cctgggaagt ggacggcacg cgggtgatct gggaaagccc
     1261 cgtcaaggag aacccggcgg tgtggttcac cgtctggccg cccgtgacgt cggtttcgcc
     1321 gatgtcggcg gtgacgaaga aggtggtggt ggtgatgggc agggtgaacc cggtccccag
     1381 cgggacgcgc gccacgccgc cggtgaagtc cgccgtgccg tgcaccgcgg cgccgaggaa
     1441 ggtgtcctgg gcggccacga agatgtcgtc gccgttgtcc agccagatgg cggcgcgcga
     1501 gaagtcgccc tgcccctggc cgatcgtcag cggcgactcc accgtgccga tctgctggag
     1561 gttgatggcg ttgaggatgg cgtagttgac gtcggtgtgc atcttcatct tcagcaccgg
     1621 cacgttctgg gtcagctggc tcaccgccgc cggcaccatg ctctccgccg agatctggat
     1681 gaggatgggg ttgatggtga cgcggctccc ctcgaaggaa tagagatagg tggtcttggt
     1741 gaccaggtcc tggtaatagc ccaccatgcc gttgggggcg gacatggtga cggcgttctg
     1801 gtccaccacc ttcacgccca cggtgttggt ggcggcggcg gagttggaga tgtcgtaggt
     1861 gaggaagaac acgcgcggcg cacctttgcc gatgaccacc gccgggctgg tggtgatggc
     1921 gatctccttc tgcttgtcgg cctccaccgc ctggttgaag gtgttggtgc ccacggagat
     1981 gagggtgtcg ccggcatcga acttcaggtt gaagttggcg tcgtcccaca cgcggatggc
     2041 ggccacgtcg ctgttggacc cttcggggag cagcgggttc acgttggaac tcccgatgcg
     2101 ctccacccgg atggcctgga actccgcctg ggacacgtcg gtctgcaggc tgaactgcag
     2161 gaacgggatg ttcaccgaac cctgtttcac gccgttcccc gtgacgaggt cccccgccag
     2221 gtcccgcacg cccagcacca cgttgtcggc ggcctcggtg atggtgccga aatcgctgtc
     2281 gctggtcagc acgtagttga cgcggtccgg cacgcccacg gtcacataat gcgacgtgcc
     2341 gatggacagg ccgagggtcc ggcccacctg ggccaggttg gagatgtcgt aggtgacgaa
     2401 gaaatactgg ccggcggcgg aggaatagat gacctccggc gtggtcaggg tgatggtggc
     2461 ggcgttggag gtgaacttct ccgtgccctg ggagagccgc gagacgctgg tgatgtcgat
     2521 ggccggttcg aagatgccgt tgtt
//
LOCUS       DAOTFD010000302         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_33530_length_2531_cov_3.632876, whole genome shotgun sequence.
ACCESSION   DAOTFD010000302 DAOTFD010000000
VERSION     DAOTFD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33530_length_2531_cov_3.632876"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5079_11480"
     CDS             complement(<1..499)
                     /locus_tag="P5079_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY30642.1"
                     /translation="MKRAKVLGLVMAGGKGERLQPLTNERGKPAVPFGGKYRIVDFVL
                     SNFVNSGIFSVYVLVQYLSQSLIDYLRVSWSNRGITQEHFITVVPPQMRLGEMWYRGT
                     ADAVSQNLNLVRDFDPDLVAVFGSDHIYRMDISQMLVYHTEKNAQVTVAARPVPFSSG
                     LGGLRR"
     gene            complement(545..1810)
                     /locus_tag="P5079_11485"
     CDS             complement(545..1810)
                     /locus_tag="P5079_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRY30643.1"
                     /translation="MPKTVKMPQPSSATEAVRLIQDQAQKDRAKIIKFFREIVAIPSM
                     DSKIGPVGRRIAKEMKALGFQNVGFDKMGNIYGRIGKGSKVLLYDSHIDTVGLGDPKA
                     WKWDPFKGKVENDILYARGACDEKCSTPGMIYGLAYAQRMGLLDGWTAYYFGNMEEWC
                     DGIAPNSLHKTEKIAPDFVVIGEPTRMQIYRGHRGRLELEVVAKGKACHASMPHLGDN
                     AIYKMSRFIQDVEALGPRIHEDPFLGRGTAVVSIIESKSPSINAVPDECTVYIDRRTT
                     FGETKESVLEQFRGLPTAKDVEIKEMFYDTPSYTGFVFKVDKYFPAWCLPEEHPLVQG
                     GLETRRLLGLDTKYPFPFKDRTPGESYRWDFSTNATYWAGKAGIPSIGFGPSNEVYAH
                     TVLDQCSLEELVDSVGFYALFPSLLKKKI"
     gene            complement(1906..>2531)
                     /locus_tag="P5079_11490"
     CDS             complement(1906..>2531)
                     /locus_tag="P5079_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30644.1"
                     /translation="FSNPCASHVSFSPTNSSHEDRSSAREEFVGEKETWLAHGFEKIR
                     FDYESRMEQLLGNVWSAAESLVGRFPAPPPVPAVSAACRVPSVPSNPLGLTDKSVRAW
                     KASPRRGEAFLRRRLAAVLSSAAGEWEGFLRSREAQLRALADEFGRQSRRAHELADRA
                     VKAALRRQADTEAAAVRRENDLRSALHAAVALREEIASFRRSLEAAG"
BASE COUNT          451 a          795 c          814 g          471 t
ORIGIN      
        1 tcctccgcaa gccgccaagg cctgaggaga aaggcaccgg ccgggccgcc acggtcacct
       61 gggcgttctt ctccgtgtga tagacgagca tctggctgat gtccatgcgg tagatgtggt
      121 cggaaccgaa caccgccacc aggtccggat cgaagtcgcg caccaggttg aggttctggg
      181 acaccgcgtc ggcggtgccg cggtaccaca tctcgcccag gcgcatctgc ggcggcacca
      241 cggtgatgaa gtgttcctgg gtgatgccgc ggttgctcca gctcacgcgc aggtagtcga
      301 tgagcgactg ggagaggtac tgcaccagca cgtagaccga gaaaatgccg gagttgacga
      361 agttgctgag gacgaagtcc acgatgcgat acttgccgcc gaacgggacg gccggtttgc
      421 cgcgttcgtt ggtgaggggc tgaagccgtt cccccttccc ccccgccatg accaacccca
      481 ggactttggc tcttttcact cgattctcgc tttcaatgga atgtcgttgc cgcgtcccaa
      541 cggctcagat cttctttttc aacagggacg gaaagagcgc gtagaacccc acggagtcca
      601 ccagttcctc cagggagcac tggtccagca cggtgtgggc gtagacctcg ttgctcggcc
      661 cgaacccgat gctcgggatc cccgccttgc ccgcccagta ggtggcgttg gtggagaaat
      721 cccaacggta ggattcgccg ggcgtgcggt ccttgaacgg gaacggatat ttggtgtcga
      781 ggcccagcag gcgccgcgtc tccagcccgc cctgcaccag cgggtgctcc tccggcaggc
      841 accacgccgg aaaatattta tccaccttga acacgaaccc cgtgtaggag ggcgtgtcgt
      901 agaacatctc cttgatttcc acgtccttgg cggtcggcag gccgcggaac tgttcgagca
      961 cggactcctt ggtctccccg aaggtggtgc ggcggtcgat gtagacggtg cattcgtccg
     1021 gcaccgcgtt gatggacggg cttttgctct cgatgatgga caccaccgcc gtgccccggc
     1081 cgaggaacgg gtcctcgtgg atgcggggcc cgagagcctc cacgtcctga atgaagcggg
     1141 acatcttgta gatggcgttg tcgccgagat gcggcatgga ggcgtggcag gccttgccct
     1201 tggccacgac ctcgagttcc agccggccgc ggtggccgcg gtagatctgc atgcgggtgg
     1261 gctcgccgat gaccacgaag tccggggcga tcttctccgt cttgtgcagg gagttgggcg
     1321 cgatgccgtc gcaccattct tccatgttgc cgaaataata cgcggtccac ccgtccaaca
     1381 gacccatccg ctgggcgtag gcgaggccgt agatcatgcc gggcgtggaa catttttcgt
     1441 cgcaggcgcc gcgggcgtag aggatgtcgt tctcgacctt ccctttgaag gggtcccatt
     1501 tccaggcctt ggggtcgccc agcccgacgg tgtcgatgtg gctgtcgtac aacaacactt
     1561 tcgatccctt gccgatgcgg ccgtagatgt tccccatctt gtcgaaaccg acgttctgaa
     1621 agcccagcgc cttcatctct ttggcgatgc ggcggcccac cggcccgatc ttggaatcca
     1681 tggacgggat ggcgacgatc tcgcggaaaa atttgatgat cttggcgcgg tctttttggg
     1741 cctggtcctg aataaggcgc accgcctcgg tggcgctcga cggttggggc atcttcacgg
     1801 ttttcggcat ggtcgggaac tcctttcaat gtagaagtga aggggcctat tgtaccgaaa
     1861 tgttacaagt taatcaatca aaagagggac gggggagcgg aaaggtcaac cggcggcctc
     1921 cagcgaacgg cgaaaggacg cgatctcctc gcggagagct accgcggcgt ggagagcgga
     1981 acgcagatcg ttctcccgcc gcaccgccgc cgcctccgta tcggcctgcc ggcgcagcgc
     2041 cgccttcacc gcccggtccg ccagttcgtg ggcccggcgc gactggcggc cgaactcgtc
     2101 ggcgagagcg cgcagttgcg cttcccggga acgcaaaaac ccttcccatt cgccggcggc
     2161 cgacgataaa accgcggcca ggcgccggcg caaaaacgcc tccccccggc gcggggaggc
     2221 cttccaggcg cgcacggatt tatccgtcaa tcccaacggg ttcgacggca ccgacgggac
     2281 ccggcaggcc gcggaaacgg ccgggaccgg cggcggcgcg gggaaccggc cgaccaacga
     2341 ctcggcggcc gaccacacgt ttccgagcag ctgttccatg cgcgattcgt aatcgaaccg
     2401 gatcttttcg aacccgtgcg ccagccatgt ctctttttcg ccgacgaatt cctcccgcgc
     2461 ggagctccgg tcctcgtggg aggaattcgt cggcgaaaaa gagacatggc tggcgcacgg
     2521 gttcgaaaag a
//
LOCUS       DAOTFD010000303         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_33579_length_2528_cov_4.150425, whole genome shotgun sequence.
ACCESSION   DAOTFD010000303 DAOTFD010000000
VERSION     DAOTFD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33579_length_2528_cov_4.150425"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="P5079_11495"
     CDS             complement(<1..532)
                     /locus_tag="P5079_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRY30645.1"
                     /translation="MSEPLFTSEDIDLLRASGIPDRDLVRQMEFFRQPPQPVKLERPA
                     TLGEGIQSLSDREVERYGQVYDAARRAGRGLKFVPASGAGTRVFKSFHVLPPEELNRE
                     SLRRSSGGGNAAAGEVLECMENLPRFPFFDDLKAGLKKRKLDVAGLLKRGQYADIVHF
                     LLDKDGLVGDLPVPTDR"
     gene            complement(529..1596)
                     /locus_tag="P5079_11500"
     CDS             complement(529..1596)
                     /locus_tag="P5079_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY30646.1"
                     /translation="MLSSGFLKYEIFHNTVLSYATGLLLFLAGVALVRVVRAIVSNRF
                     KAWAEKTATTLDDFLLQAFEKSAVPLFYYGAFYFALSGLVLPPSLRKGLALLTTVVLT
                     VCAVQFVVELVASLLNEYWLKKERGSSREQVVKGLLPFLKTLIWGLGAVFLLDNLGYK
                     VSTVLAGLGIGGVAVALAAQAILGDLFSYFAILFDRPFVIGDFIVVDDAMGTIEHIGI
                     KTTRVRSLSGEQLVFSNSSLTASCIRNYKRMEQRRVLQKISVAYATPTPQLKEIPEML
                     KAIVSPRKDVRFDRAHLCAFSDFSIDFELVYYVLSGDYNQHMDIQQEILLAVKEYFDR
                     RGIEIPFPTQTLFVKKTAGGA"
     gene            complement(1707..>2528)
                     /locus_tag="P5079_11505"
     CDS             complement(1707..>2528)
                     /locus_tag="P5079_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY30647.1"
                     /translation="EVATLLARSVLPQRINDPMCGFFVLRRGVYEKIRDRLRPRGYKI
                     LLEIVHRASPLRVREFPYVFRDRKSGYSKLSAFVAVSYLESLFELRFGFSPLDRLREK
                     YHRGRYKIVEGMLGGGTLLDVGCGRPCDSMPDGALLEHLGRGVGLDIKACVGRSEFVR
                     GGLLQMPFRSGAFDRVSALEVLEHMDDLPAALAEVDRVLKPGGVFVVSVPDDNLAWKC
                     IWWVWVKLIGRMWRHTHHTHFQAAQWRRVLSERFDVVSFRRHWYFDLVFQLRRRG"
BASE COUNT          580 a          787 c          733 g          428 t
ORIGIN      
        1 cccggtcggt cgggaccggc aagtctccca ccaggccgtc cttgtccagc aggaaatgga
       61 cgatgtccgc gtattggccg cgtttgagaa gaccggcgac gtccagcttc cttttcttga
      121 ggccggcctt caggtcgtcg aaaaacggga accttggcag gttctccatg cattcaagca
      181 cttccccggc cgccgcgttc cccccgccgg acgagcgccg gagactctcc cggttcagct
      241 cttcgggcgg caagacgtga aacgatttga acacgcgcgt gccggcgccg gaggcgggaa
      301 cgaacttaag gcctcgtccc gctcgtcggg cggcgtcgta cacctgcccg taacgctcca
      361 cttcccgatc ggaaagggac tggatcccct cccccaacgt ggccggccgt tccaatttga
      421 cgggttgggg cggctgacgg aagaattcca tctggcggac gaggtcccgg tccgggatgc
      481 cgctggcgcg gagaaggtcg atgtcttccg acgtgaagag gggctcgctc atgcgccccc
      541 cgctgttttt ttgacgaaaa gggtctgcgt gggaaacggg atttctatcc cccgacgatc
      601 gaaatactct ttaacagcca aaagaatctc ttgctgaatg tccatgtgct gattatagtc
      661 gccggacaga acataataga ccagctcgaa atcaatactg aaatcgctga aagcacacag
      721 gtgcgcccga tcaaatcgca cgtcctttcg gggggaaacg atcgccttga gcatttcggg
      781 tatctctttc aactgcggcg tgggcgtagc ataggcaaca ctgattttct gcaacacccg
      841 cctctgttcc atgcgcttgt agttgcggat gcaggaagcc gtaaggctgg aattggaaaa
      901 aaccaattgc tcgccgctga gactgcggac gcgggtggtc ttgatgccga tgtgctcgat
      961 ggtgcccatg gcgtcgtcaa caacgatgaa gtcgccgatg acgaatggcc ggtcgaacag
     1021 gatggcgaag tagctgaaaa ggtcgcccag gatggcctgg gcagccagcg ccaccgcgac
     1081 gccgccgatc cccaaccccg ccagcaccgt ggagacctta tagccaaggt tgtccagaag
     1141 aaaaacggca cccagccccc agatcaatgt tttcaggaag ggcaggagcc ccttgacgac
     1201 ctgttcacgc gaggaccccc gttctttctt cagccagtat tcgttgagga gggaggcgac
     1261 gagttcgacg acgaactgga cggcgcagac cgtcaacacc accgtcgtca acagcgccag
     1321 ccctttgcgc agagacgggg gcagaaccag cccggagagt gcaaaataga acgcgccgta
     1381 gtagaagagc gggacggcgg atttttcgaa cgcctgaagg agaaaatcat ccagggtggt
     1441 ggcggtcttt tccgcccacg ccttgaaccg gttactgacg atggcgcgaa cgacgcgcac
     1501 caacgccacc ccggcaagaa agaggaggag ccccgtcgca taggagagga cggtgttgtg
     1561 gaatatttcg tatttaagga aaccgcttga aagcatcaga ccctcctcca ccgccggcaa
     1621 aaggccggca accgttgaaa tcgcaagaaa ttatagcaaa ggcccccggc ggccgacggg
     1681 aaagcgccgg cccgcccgaa cgggactcag cctctccggc gcagctgaaa aacgaggtca
     1741 aaataccagt gacgccggaa agaaaccacg tcgaacctct ccgacaacac ccggcgccac
     1801 tgggcggcct gaaaatgcgt gtgatgggta tggcgccaca tccggccgat cagtttcacc
     1861 cacacccacc agatgcattt ccaggccaga ttatcgtcgg gcacggagac cacaaaaaca
     1921 ccgccgggct tcaaaacgcg gtccacctcg gccaaggccg ccggcaagtc gtccatgtgc
     1981 tccaaaacct ccagcgccga cacccgatcg aacgccccgg accggaacgg catctggaga
     2041 agcccgcccc ggacaaactc ggaccgcccc acgcacgcct tgatgtcgag ccccacgccg
     2101 cggccgaggt gttccaacag cgcgccgtcg ggcatggaat cgcagggacg gccgcacccc
     2161 acgtcaagca aagttccccc gcccaacatc ccctccacga tcttatagcg tccgcggtgg
     2221 tatttctccc tcagacgatc gagaggactg aatccgaaac ggagttcgaa cagggattcc
     2281 aggtaactga cggccacgaa cgccgaaagt ttggaataac cgcttttacg gtcgcggaac
     2341 acatagggga attccctgac gcggagcggg ctcgcccgat ggacgatctc cagcaatatc
     2401 ttgtaaccgc gtgggcggag ccgatcccgt attttttcat agacccccct ccgcaaaacg
     2461 aagaaaccgc acatgggatc gttgatcctc tggggcagga cggaccgggc cagcaaagtg
     2521 gccacttc
//
LOCUS       DAOTFD010000304         2516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_33848_length_2516_cov_2.980496, whole genome shotgun sequence.
ACCESSION   DAOTFD010000304 DAOTFD010000000
VERSION     DAOTFD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33848_length_2516_cov_2.980496"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1131
                     /locus_tag="P5079_11510"
     CDS             <1..>1131
                     /locus_tag="P5079_11510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRY30648.1"
                     /translation="GPPAAALPPTRTLLTPFKTALGFEFTAAQKRVINEIFADMSRTK
                     PMNRLLQGDVGSGKTVVALSAMLLAVENGRQAALMVPTEILAEQHFLTLKKFFNNLPV
                     SWALFSRGVPAAQRRRQEEQLRTGEIQIAIGTHALIQETVAFKDLGLVVVDEQHRFGV
                     LQRAELQRKSQSPHVLIMTATPIPRTLALTLYGDLSTSVIDELPPGRPAITTHWTLED
                     NAFAAIRQTVKEGKQAYIVVPLVEESEKLDLRAAVKEWERLKTRVFPEFTVGLLHGRM
                     KPQEKEAAMAQFMAGQTHIMVATPVVEVGIDVANATVMMVMDAERFGLAQLHQLRGRV
                     GRGKESSACYLVSDPKSADAAQRMRLICMTQDGFKLAEEDLK"
     assembly_gap    1132..1141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1142..1397
                     /locus_tag="P5079_11515"
     CDS             <1142..1397
                     /locus_tag="P5079_11515"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HRY30649.1"
                     /translation="DGFEIAENDLKLRGPGEFLGESQHGLPPLKVGNIVRDGALIEEA
                     REAAFQLITTDSRLKKEENRPFLQELRARFGTKLAFGQVA"
     gene            1472..1978
                     /gene="coaD"
                     /locus_tag="P5079_11520"
     CDS             1472..1978
                     /gene="coaD"
                     /locus_tag="P5079_11520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRY30650.1"
                     /translation="MANKKRIVVYPGSFDPVTNGHLDIVHRACRLFDHVVVAVSQNPL
                     KTCLFSLPERKRFLTLALAPLIRQGKVSVELFDGLLVHFAQKKGAEAVVRGLRAISDF
                     EYEFQMALMNRHQDSSVETVFLMPDEKYTYLSSTVIKEIVRLGGKIERFLPAAIVPEI
                     RKKLRPAR"
     gene            2137..>2516
                     /locus_tag="P5079_11525"
     CDS             2137..>2516
                     /locus_tag="P5079_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRY30651.1"
                     /translation="MLSAASFLAERKIAVPVLLGDRRAVENAATEGGFPLPGVEILDM
                     AGLPDKNGLAQKLFERRKTKGLSLEAAHGLLTDPLYAAAMLLAVGSADGLLAGAVRST
                     ADTVRAAFACLGLARGAETIFGAFL"
BASE COUNT          484 a          854 c          753 g          415 t
ORIGIN      
        1 aagggccgcc ggccgccgcc cttccgccca cccggacgct cctgacgccc ttcaagacgg
       61 ccctcgggtt cgaattcacc gccgcccaga aacgggtcat caacgaaatc ttcgcggaca
      121 tgtcccgcac caaacccatg aaccgcctcc tccagggcga cgtgggctcc ggcaaaaccg
      181 tggtggcgct gtccgccatg ctcctggccg tggagaacgg ccgccaggcc gcgctgatgg
      241 tgccgacgga gatcctggcg gaacaacatt tcctcaccct gaaaaaattc ttcaacaacc
      301 tgccggtgtc ctgggcgctc ttcagccgcg gggtgccggc cgctcaacgc cgtcggcagg
      361 aggagcagct gcggacgggg gagatccaga tcgccatcgg cacgcacgcc ctcatccagg
      421 agaccgtggc cttcaaggac ctcgggctcg tcgtggtgga cgaacaacac cgtttcggcg
      481 tgctgcaacg cgcggagctc cagcgcaaat ctcagtcgcc ccacgtgctc atcatgaccg
      541 ccacgcccat cccccgcacg ctggcgctca cgctctacgg cgacctctcc acctccgtca
      601 tcgacgagct cccgcccggc cggcccgcca tcaccaccca ctggacgctg gaggacaacg
      661 ccttcgccgc catccggcag accgtgaaag aaggaaaaca ggcctacatc gtggttcctt
      721 tggtggagga gtcggaaaag ctcgacctcc gcgccgccgt caaggagtgg gagcggctga
      781 agacccgcgt cttcccggaa ttcaccgtcg gacttttgca cggccgcatg aaaccccagg
      841 aaaaggaggc cgccatggcg cagttcatgg cgggccagac gcacatcatg gtggccaccc
      901 cggtggtgga ggtgggcatc gacgtggcca acgccacggt gatgatggtg atggacgcgg
      961 aacggttcgg cctggcccag ctgcaccagc tgcgcggacg cgtggggcgg gggaaagaat
     1021 cgtcggcctg ttatctggtg tccgacccga aatccgccga cgccgcccag cgcatgcggc
     1081 tcatctgcat gacccaggac gggttcaaac tggccgagga ggacctgaaa cnnnnnnnnn
     1141 ntgatggctt tgagattgcc gaaaatgact taaaactccg cggccccggc gaattcctcg
     1201 gcgaatcgca gcacggcctg ccgccgctga aagtgggcaa catcgtgcgc gacggggccc
     1261 tcatcgagga ggctcgggag gcggcgttcc aactcatcac cacggacagc cgcctgaaaa
     1321 aagaggagaa ccggccgttc ctgcaggaac tccgcgcgcg tttcggcacc aaactcgcct
     1381 tcggccaggt ggcgtgagcc ctcgcaaatt caccgcgtct tcgccgacaa atccattgac
     1441 atttccggcg acgaaagttt agaatctctc cgtggcgaac aaaaaacgca tcgtggtcta
     1501 tccgggcagt ttcgatcccg tcaccaacgg gcacctggac atcgtccacc gagcctgccg
     1561 tctcttcgac cacgtggtgg tggcggtgag ccaaaacccg ctgaaaacct gcctcttctc
     1621 ccttccggaa cgcaaacggt tcctcaccct ggcgctggcc cccctgatcc ggcaggggaa
     1681 agtgtccgtg gaactgttcg acggactgct cgtgcatttc gcgcagaaaa agggcgcgga
     1741 ggcggtggtc cgcggattgc gcgccatctc ggatttcgag tatgaattcc agatggccct
     1801 gatgaaccgg caccaggact cctccgtgga aacggtcttc ctcatgcccg acgaaaaata
     1861 cacctacctt tcttccaccg tcatcaagga gatcgtccgg ctcgggggga aaatcgagcg
     1921 gttcctgccg gccgccatcg tcccggaaat ccgaaaaaaa ctgcgccccg cccgctgaac
     1981 ctctcccccg acgcgctctt gacaacaccc catccccgtg ttattcttaa aaaagtccgc
     2041 gaaaactcgc gcggttctgg attttgggac gcctggagac gccgggcctc ggaaaaaagc
     2101 cgtcgcgtta ttttccccga atccacggac ccgcgcgtgt tgtcggcggc gtcctttctg
     2161 gcggaacgga aaatcgccgt ccccgtcctg ctgggcgacc ggcgggcggt ggaaaacgcc
     2221 gcaaccgagg gcggattccc cttgcccggc gtggaaatcc tggacatggc gggcctgccc
     2281 gacaaaaacg gtctggccca aaaacttttt gaacgccgaa aaaccaaagg gctgtccctg
     2341 gaagccgccc acgggctgtt gacggacccg ctgtacgccg cggccatgct tctggccgtc
     2401 ggctcggccg acggcctcct ggcgggcgcg gtgcggtcca ccgccgacac cgtgagggcg
     2461 gccttcgcgt gcctcgggct ggctcgcggg gcggaaacga tcttcggcgc gttcct
//
LOCUS       DAOTFD010000305         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_33920_length_2512_cov_4.065527, whole genome shotgun sequence.
ACCESSION   DAOTFD010000305 DAOTFD010000000
VERSION     DAOTFD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33920_length_2512_cov_4.065527"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2512
                     /locus_tag="P5079_11530"
     CDS             <1..>2512
                     /locus_tag="P5079_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30652.1"
                     /translation="IAISELKGTMDFLNGREVVFRDREGKEYTRVDLVELAGTPGGAE
                     AAAILGWYAGLALNAKVNSDGTVTLNIGGEIPVKVNLDTFLQTQVAISELAGAKAYLD
                     DKTIVFRDRNGREFSRVDLLTAAGELTPEGVKAAAILGWYGGLALHASVNPDGTVTLK
                     ITDEKGRSLPTPKSVDLDTFLKTQVAISQLGGAMKFLDDGTVVYYDRTGKAYTRVDLL
                     AEAGQGTPAAAQAAAILGWYAGLALSSTVTPEGKVMMTIGGKTPMEVDLDTFLRTQAA
                     ISELAGAQAFLDGNTVVFRDRNGREYSRVDLLTEAGQTTPEGIKAAAILGWYGGLALQ
                     SEIRDGKVFLHITDAEGNKLSVPESVDLDTFLKVQAAISELPGAQKFLDDGVVVFKDK
                     TGREFTRVDLLELAGTKEGATAAAILGWYAGLALSSRVNADGTVSLTIGGEVPVEVDL
                     DTFLATQAAISELAGAKAYLDANTIVFVDAQGKEYSRVDLLGDAGNATPAGARAAAIL
                     GWYAGLALHSEIKDGKVILNITDADGNSLETPAEVDLDTFLKIQAAISELGGAKEFLD
                     DGTVVFKDRNGKEFTRVDLVTLAGTPEGAKAAAILGWYAGLALNSKVNEDGTVTLTIG
                     GTVPVSVDLDTFLRTQVAISELAGAKAYLDDKTIVFVDREGKEYSRVDLLTASGQLGP
                     EGVKAAAILGWYAGMALHSSVTADGRVILQLTDENGNQLPTPATVDLDTFLKVQIAIS
                     ELSWAKKYLDDGTVVFYSKDGKGYSRVDLVALAGSGTPEGTLAAAVLGWYAGLALRVE
                     ATATGLVIHFYKDKDGDGQADEGEEIATPQSIDLNQFLR"
BASE COUNT          481 a          768 c          872 g          391 t
ORIGIN      
        1 atcgccatca gcgagttgaa ggggaccatg gatttcctga acggccggga ggtggtgttc
       61 cgcgaccggg aggggaagga atacacccgc gtggatctgg tcgagttggc ggggacgccc
      121 ggcggcgccg aggcggcggc catcctgggc tggtacgccg ggctggcgtt gaacgccaag
      181 gtcaacagcg acggcaccgt caccctgaac atcggcggcg aaatccccgt caaagtgaat
      241 ctggacacgt tcctgcagac gcaggtggcc atcagcgaac tggccggcgc caaggcctat
      301 ctggacgata agaccatcgt ttttcgggac cggaacggcc gtgagttttc ccgggtggat
      361 ctgctgacgg cggcgggcga gctcacgccg gagggcgtga aggcggccgc catcctcggc
      421 tggtacggcg ggctggcgct ccacgcttcc gtcaaccccg acggcaccgt gacgctgaag
      481 atcaccgacg agaagggccg cagcctgccc acgcccaaga gcgtggacct ggacacgttc
      541 ttgaaaaccc aggtggccat cagccagctc ggcggagcca tgaaattcct ggacgacggc
      601 accgtcgtct attacgaccg caccggcaag gcctataccc gggtggacct gctcgcggag
      661 gcgggccagg ggaccccggc ggcggcgcag gcggcggcca tcctgggctg gtacgccggg
      721 ctggcgttga gttccacggt gacgccggag gggaaagtga tgatgaccat cggcggaaaa
      781 acgccgatgg aggtggacct ggacaccttc ctgcgcaccc aggcggccat cagcgaactg
      841 gccggcgccc aggctttcct ggacggcaac accgttgttt tccgcgaccg caacggccgc
      901 gaatattccc gcgtggactt gctcaccgag gcgggacaga ccacgccgga aggcatcaaa
      961 gcggcggcca tcctgggctg gtacggcggg ctggcgctcc aatccgagat acgggacggg
     1021 aaagttttcc ttcatatcac cgacgcggag gggaataaat tgtcggtgcc ggagagcgtg
     1081 gacttggaca cgttcctgaa ggtgcaggcg gccatcagcg aattgcccgg cgcgcagaaa
     1141 tttctcgatg acggcgtcgt cgtcttcaag gacaagacgg gccgggagtt cacccgggtg
     1201 gaccttctgg aattggcggg caccaaggag ggcgccaccg ccgcggccat cttgggctgg
     1261 tacgccgggc tggccctgag ttccagggtg aacgccgacg ggaccgtgtc tctgaccatc
     1321 ggcggcgagg tcccggtgga ggtggacctg gacaccttcc tggcgaccca ggcggccatc
     1381 agcgaactgg ccggcgccaa ggcctatctc gacgccaaca ccatcgtttt tgtggacgcg
     1441 caagggaaag aatattcccg cgtcgacttg ttgggagatg ccggcaacgc cacgccggcc
     1501 ggggcccgcg ccgccgccat cctcggctgg tacgccgggc tggccctcca ttcggaaatc
     1561 aaggatggaa aagtgatttt gaacatcacc gacgccgacg ggaacagttt ggaaacgccg
     1621 gcggaggtgg acctggacac gttcctgaag attcaggcgg ccatcagcga actgggcggc
     1681 gccaaggagt tcctcgacga cggaaccgtg gtcttcaaag accggaacgg caaggagttc
     1741 acgcgcgtcg acttggtgac gctggccgga acacccgaag gggccaaggc cgcggcgatc
     1801 ctgggctggt acgcggggct ggcgctcaat tccaaggtga acgaagacgg gaccgtgacc
     1861 ttgaccatcg gcggcacggt gccggtgtcg gtggacctgg acacgttcct gaggacccag
     1921 gtggccatca gcgaactggc cggcgccaag gcctacctgg atgacaaaac catcgttttc
     1981 gtggatcggg aagggaaaga gtattcccgc gtggatctgc tcacggcctc cggccaactc
     2041 ggtccggaag gcgtcaaggc tgcggcgatc ctgggctggt acgccgggat ggccctccat
     2101 tcctccgtca ccgccgacgg ccgggtgatt ctccagctga cggacgagaa cgggaaccag
     2161 ttgccgaccc ccgccaccgt ggacctggac acgttcctga aagtgcagat cgccatcagc
     2221 gagctgtcgt gggccaaaaa atatctggac gacggcacgg tcgtcttcta cagcaaggac
     2281 ggaaagggct actcccgcgt ggatctggtg gctctggccg gcagcggcac gccggaaggg
     2341 accttggccg ccgcggtgct cggttggtat gcggggctgg ctctgcgcgt ggaagccacc
     2401 gccaccgggt tggtcatcca tttttataag gacaaggacg gcgacggcca ggcggacgag
     2461 ggggaggaaa tcgccacccc gcagagcatc gacctgaacc agttcctgcg cc
//
LOCUS       DAOTFD010000306         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_34469_length_2487_cov_3.790707, whole genome shotgun sequence.
ACCESSION   DAOTFD010000306 DAOTFD010000000
VERSION     DAOTFD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34469_length_2487_cov_3.790707"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2487
                     /locus_tag="P5079_11535"
     CDS             <1..>2487
                     /locus_tag="P5079_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30653.1"
                     /translation="QLRNETGQYWNGGWSGSESWPAATLAGGAWSYTHGASFTDGARY
                     TLASRAVDAASGVESSFTVGVNSMTFTYDTTAPSSPGGLAVTNPGLDATLALGWTANA
                     ETDLAGYAIYRATYSFSSHVDPYVVFVASVSSNTLTYQDSSGLTNGTTYHYALWAYDQ
                     SLPTPNLGPLCATDSDYPTKGAHHLEISGLPGSVTAGTPFNATLTVHDPEHNIVGSYR
                     GTVTWSNTEGVGFAPPQTTVVPGNYNFTAGDLGVHAFTGGFVLKAAGWRYLQAVDITS
                     APISTERSGFSSRPYVRVDPDVPHHFDSLSPSSDQYVSAGYSTFIGSIPVSARLVDVY
                     DNPVATGTPVNVAVTGVSGTAGTLKDIYGSVVASTATDTLGYVGLAPALYYEVSTTFG
                     DYAQVQFSTSSISSATARVMTLPGPTTKFVLTGRPVSQLAGNDSGLFTVERRDYFENA
                     TTDTVITADLVTDSTSPAPSKFKNAGGTPITAVTFGLGAASAQFRYYDTLASTHTISV
                     STAGLTAGTTSYGVLPGAATQLLTVLPGQSYTPGADPGRSGTALPQTAGVPFGGVAVR
                     AVDAYWNTDPSVGSSVWLNSTDAYAALPAAQPLIEGVTSFLLTPIVAGTQTVTASDGG
                     TLAGVASSTYTVYASTATKLQVLVPGEARLPGSARGKTGTAAEQNRNIPFTVTVNLTD
                     DYYNLQSTDTAVPLLVTSSDTADANHSLTLAAYSVSTETSHVFLTETLAGWDILVATT
                     NPMQAFSGDASTGIVVRSTISAPTIQPAVALSSDAIKWLWLDNSDNELGFSLRTGGDV
                     WIASATIPGTTTYEETGLTPNTLYTRLVRA"
BASE COUNT          417 a          838 c          789 g          443 t
ORIGIN      
        1 ttcagttgag gaacgagacg gggcaatatt ggaacggcgg ctggtcgggg agcgaaagct
       61 ggcccgcggc cactctggcg ggcggcgcgt ggagctatac gcacggcgcg agcttcacgg
      121 acggggcgcg gtacactctg gcgagccgcg cggtggacgc ggcctccggc gtggagtcgt
      181 cttttacggt gggggtgaat tccatgacgt tcacctacga caccacggcg ccctcctccc
      241 ccggcgggtt ggctgtgacg aaccccggcc tggacgccac cttggcgttg ggatggaccg
      301 ccaacgccga gaccgacctc gccggctatg cgatctaccg ggccacgtac tcgttctcgt
      361 cccacgtcga cccgtacgtc gtgtttgtgg cgtcggtgtc cagcaacacg ctgacgtatc
      421 aggactcgtc cggattgacc aacggcacga cctatcatta cgccctgtgg gcctacgacc
      481 aatcgttgcc cacgcccaac ctcgggccgc tgtgcgccac ggattccgat tatcccacca
      541 agggggcgca tcacttggag atcagcggtt tgcccgggtc ggtgacggcc ggaacgccgt
      601 tcaacgcgac tctgacggtc catgaccccg agcacaacat cgtcgggagt tatcgaggaa
      661 cggtgacctg gtccaacacg gaaggggtgg ggttcgcgcc cccacagacg accgtcgtgc
      721 ccggcaacta caacttcacg gcgggggacc tgggggtgca tgcctttacc ggtggtttcg
      781 tgctcaaggc cgccggttgg cgttatctgc aggcggtgga tatcaccagc gctcccatct
      841 ccaccgagcg ttccggattc tcttcccgcc cctacgtgcg ggtcgatccc gatgtcccgc
      901 accacttcga cagtctttct ccgtcgtccg accaatacgt gtcggccgga tattcgactt
      961 tcatcgggag tattcccgtt tcggcccgcc tcgtggacgt ctacgacaac ccggtggcca
     1021 cgggcacgcc ggtgaacgtg gcggtgacgg gggtgagcgg aacggccggt acgttgaagg
     1081 acatttacgg gagcgtggtc gccagcaccg ccacggacac gctgggttat gtgggcctgg
     1141 ccccggcgct ttattatgaa gtgtccacga ccttcggcga ttatgcccag gttcaattct
     1201 ccacgagttc catttcctcc gccacggcgc gggtgatgac gctgcccggt cccacgacca
     1261 agttcgttct gaccggtcgt ccggtgtctc aactggccgg gaacgacagc ggacttttca
     1321 ccgtggagcg gcgcgactat tttgaaaacg ccaccaccga taccgtgatc acggccgatc
     1381 tggtgaccga ctccaccagc ccggcgccca gcaaattcaa gaacgccggc gggacgccca
     1441 tcaccgcggt gacgttcggc ttgggggccg ccagcgccca gttccgctac tacgatacgc
     1501 tggccagcac gcacacgatt tccgtgagca ccgcgggttt gacggccggg acgaccagct
     1561 atggggtcct ccccggcgcg gccacccagt tgttgacggt gttgccggga cagtcctaca
     1621 cgcccggggc cgatccgggg cggagcggca cggcgttgcc ccagacggcc ggcgtgccct
     1681 tcggcggcgt tgcggtgcgg gccgtggacg cctactggaa caccgacccc tcggtgggca
     1741 gcagcgtttg gttgaactcc accgatgcgt atgcggcgct ccccgccgcc cagccgttga
     1801 tcgagggcgt caccagcttc ctgttgacgc ccatcgtggc gggaacacag accgtcaccg
     1861 ccagcgacgg cgggacgctg gcgggcgtgg cgagttccac ctacaccgtc tacgcctcca
     1921 ccgccacgaa actgcaggtt ttggtcccgg gggaggcccg gctgcccggt tcggcgcgcg
     1981 gtaaaacggg gaccgccgcc gaacagaacc ggaacatccc gttcacggtc acggtgaacc
     2041 tcaccgacga ttattacaat ttgcaaagca ccgacaccgc ggtgcctctc ctcgtgacgt
     2101 cttccgacac cgcggacgcc aaccattcgt tgaccctggc ggcttacagc gtgtcgacgg
     2161 agacttccca tgtctttttg accgagacgc tggcggggtg ggacatcctg gtggccacca
     2221 ccaacccgat gcaggcgttc tccggcgacg cctccaccgg catcgtggtg cgcagcacca
     2281 tttcggcgcc caccatccag ccggcggtgg cgctgtcgtc ggacgccatc aagtggctct
     2341 ggctggacaa cagcgacaac gaactggggt tcagtctccg caccgggggc gatgtgtgga
     2401 tcgcctcggc gaccatcccg gggaccacaa cctatgagga aaccgggttg acgcccaaca
     2461 ctctctacac gcgcttggtc cgagcgt
//
LOCUS       DAOTFD010000307         2479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_34639_length_2479_cov_5.355611, whole genome shotgun sequence.
ACCESSION   DAOTFD010000307 DAOTFD010000000
VERSION     DAOTFD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34639_length_2479_cov_5.355611"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="P5079_11540"
     CDS             <1..253
                     /locus_tag="P5079_11540"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRY30654.1"
                     /translation="VERAPAPPSRRVTLTLPLINRARRIFFMVTGKKKAGILADIFGG
                     TRPRLPAQRVHPRSGGSAYWLDAAAARSFPSLPHGKMNR"
     gene            250..1716
                     /locus_tag="P5079_11545"
     CDS             250..1716
                     /locus_tag="P5079_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30655.1"
                     /translation="MKRGLQRGVLVAAGVLLSLALAEIGLRFAGSLSLRRQERRNMRA
                     LSAKGTYRILCLGESTTAYGGENSYPSQLEKILNARAGGVRFAVVNRGVPGTDSARIA
                     DGIDDNLDRYAPAMVVTMMGANEETVAAQKRAAGKQNGRPWWDRFGMGRLVESVVGGF
                     SARAFPGEGMEQVRNERGERLVYEGRYDEAEEFYRKAIARDPQGVKARLELAALYLRQ
                     DRFQEAESLLKKVSEIEPKNVEAVRLSAMGLRFQGRPAEAEKRLRAAMDNGIDDVILY
                     VELSLSCAQQSRCAEAERVLVEKIAAGASPAWPAIHRLANFLLGREEYEETEKLLREL
                     MVRGYEDHRAWGLLATLMQEQGKYGLAEEFYAKSTALRKAHANQELRRNYRVLRDKVR
                     GRGIRLVCVQYPVRDVGPLVEMLAPDEGVFVVDNEKLFKEALKKYKYAELFKDRFGGD
                     FGHGTPLGNRLLAENVAETILAARSPEEGPVLQQEGGR"
     gene            1990..2421
                     /locus_tag="P5079_11550"
     CDS             1990..2421
                     /locus_tag="P5079_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004974501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY30656.1"
                     /translation="MTKKLAKSVKGFTLIELMIVVAIIGILAAIAIPKFADLVTKSKE
                     SAVKGSLGSVRSAMSIYYSDTEGVFPALGNLVTALCTGSKYLKELPYIQVPKPGNHGS
                     VNSETNTLGDGGNWYYPSVTEGHVAVNCTHTDSKNSVWSSW"
BASE COUNT          518 a          653 c          832 g          476 t
ORIGIN      
        1 ggtggagagg gcccccgccc cgccgtcgcg ccgcgtcacc ctcacgctgc ccctcatcaa
       61 ccgggcgcgt cgcatttttt ttatggtgac gggtaaaaaa aaggccggga ttctggcgga
      121 catctttggg ggaactcgcc cgcggcttcc cgcccagcgg gtgcatccgc gctccggggg
      181 ttcggcctac tggctcgacg ccgccgccgc gcggtcgttc ccctctcttc cgcacgggaa
      241 aatgaaccga tgaaaagagg gttgcaaaga ggggtcctcg tcgccgcggg cgttctcctg
      301 tcgctggccc tggcggagat cggcctgcgt ttcgccggtt cgttgtcgct gcggcggcag
      361 gaacgacgga acatgcgggc gttgtcggca aagggaacct atcgcatttt gtgcctcggc
      421 gagtccacga cggcgtacgg gggagagaac tcctatccca gccaactgga aaaaatattg
      481 aacgcccgtg cgggaggagt caggttcgcc gtagtgaacc ggggtgttcc cggcacggac
      541 tccgcacgga ttgcggacgg gatcgacgat aacctggaca gatacgcccc ggccatggtt
      601 gtgaccatga tgggggccaa tgaggagacg gtggccgctc aaaagcgcgc cgccggcaag
      661 cagaacggaa ggccgtggtg ggacagattc gggatgggga ggttggtgga atcagtggtc
      721 ggcggatttt cggcgagggc gtttccgggg gaggggatgg aacaggttcg gaacgagagg
      781 ggggagcggc tcgtttatga gggaagatac gacgaggcgg aggaattcta ccggaaggcg
      841 atagcgcgcg atccccaagg agttaaggcc cggcttgaac tggccgccct ttatctgcgg
      901 caagaccgat ttcaggaagc ggagtcgttg ctcaagaaag tttcggagat tgaaccgaag
      961 aatgtcgagg cggtccggct gtcggccatg ggcctgcgtt tccagggccg gccggccgag
     1021 gcggaaaaaa gattgcgcgc ggccatggat aacgggattg acgatgtgat tttatacgtc
     1081 gaactttcgt tgagttgtgc ccaacaaagc cggtgcgccg aggcggaacg cgttcttgtt
     1141 gaaaaaatcg ccgccggggc ttctcccgcc tggcccgcca tccatcggct ggccaatttc
     1201 ttgttgggtc gtgaggaata cgaggaaacg gagaaacttc tgagggagtt gatggtccgg
     1261 ggctacgagg accatcgggc ctgggggctg ctggccaccc tcatgcagga gcaggggaaa
     1321 tacgggttgg cggaagaatt ttatgcaaaa tcgacggccc tgcggaaggc ccatgccaac
     1381 caggaacttc gccggaatta tcgggtcttg agagacaagg ttcgggggcg ggggatacgg
     1441 ctggtttgcg tgcaataccc ggttcgggac gtcggaccgt tggtggagat gttggcgccg
     1501 gacgaggggg ttttcgtcgt cgataacgag aagctcttca aggaggcgct caaaaaatat
     1561 aaatacgcgg agctgtttaa ggaccggttc ggcggggatt ttggacatgg cacgcccttg
     1621 gggaaccggc tcttggccga aaacgtcgcc gagacgatcc tcgccgcccg gtctccggaa
     1681 gaaggaccgg tcctgcaaca ggagggcggg cggtagcgta aagtcacact tgaaaacgac
     1741 ctgttcgggg gttatactta aaacgaaggg cgcctccggg acggggtatt ttagccatcc
     1801 ttcatcctca cgttcttccc gcccggtgtt ggaatgcctt tccgtgttat tcgcaggacc
     1861 gtttcgtcga aaacatcctt tgttttcggc ggggattttt gtgaacaact tccgagccga
     1921 ctcctccccg agacccgcgt tttgctccga acggatttcc atgcctcaat ggaaaggggg
     1981 tgaacgacaa tgacaaaaaa actcgcaaag tcggtgaaag ggttcacgct catcgaactg
     2041 atgatcgtgg tagccatcat aggtatcctg gcggccatcg ccatccccaa attcgccgac
     2101 ctggtgacga agtcgaagga aagcgcggtg aagggaagcc tcggttccgt ccgcagcgcc
     2161 atgagcatct attactcgga cacggaaggt gtgtttccgg ccctgggtaa cctggtgacg
     2221 gcgttgtgca cgggctcgaa gtacctgaag gaattgcctt acattcaggt cccgaaaccg
     2281 ggcaaccacg gcagcgtcaa ctccgagacc aacaccttgg gtgacggcgg caactggtat
     2341 tacccgagcg tgacggaagg gcacgtggcg gtcaactgca cgcacacgga ctccaagaac
     2401 tcggtgtgga gcagctggta agctgaggga atcgaattac aaagacggga gaggggggaa
     2461 cacctcctct cccgtcttt
//
LOCUS       DAOTFD010000308         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_35686_length_2433_cov_3.530698, whole genome shotgun sequence.
ACCESSION   DAOTFD010000308 DAOTFD010000000
VERSION     DAOTFD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35686_length_2433_cov_3.530698"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="P5079_11555"
     CDS             complement(<1..1160)
                     /locus_tag="P5079_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRY30657.1"
                     /translation="MSTKVGALRVFIIDGDHAFVKAFAERFKQLSVYTVMVDICPTGA
                     EMLDRIYRQSYDLIFLDMNLADMDSLEVLSRVGQTTPPLPLVMMGGTNNARMAVEAMK
                     RGILDFLMKDDLLTLDLAGLIQRLMDSFRLRRENAELVQINQMKDDFLATISHELRTP
                     LTSILGLSEVLLTGRMGTLEEKQAQSLKKILEQSHLLVKLINQLLDIRALSQGGAMLA
                     MKPISLRELAIKQATTMQTLFDKKGVQLIFENTEEPLFVQADPDSLSKVFEHLVSNAL
                     KFTPREGKVFVETKSIENGQAQFRVRDTGRGIPSEALPYVFQKFFHVDKTLTRPYGGM
                     GLGLAFCREVVEAHSGRVWVESKGLGQGTSVTVLLPVLRDHVAAVDKKAAPAA"
     gene            complement(1302..2246)
                     /locus_tag="P5079_11560"
     CDS             complement(1302..2246)
                     /locus_tag="P5079_11560"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRY30658.1"
                     /translation="MTVLRNARGRYVSGSRLAAGLCLTRAAIHKQVVRLREWGYDITG
                     TNRKGYRLENSPQALDTDRFRAASLGRPICHFPSLGSTQDELKERALAGAPQGTLVLA
                     DEQTSGRGRMGRRWLSPRGGLWFSLLLRPAIPPAQVPALALVAALDWAMVLRRATGLP
                     ACVKWPNDVWIDNRKVAGILTEMSSEMDRVHWVVLGVGVNINNPSPRGMTPPAASLRE
                     MTGKPFYRQALLEDWLKRFAGSLRRYQRDGFSAFRRDCEECLSMKGRTVVCETAQGTV
                     AGRVEGIDGEGRLLLRTAAGLRRFGEGAIRLKKGDERA"
     gene            complement(2306..>2433)
                     /locus_tag="P5079_11565"
     CDS             complement(2306..>2433)
                     /locus_tag="P5079_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRY30659.1"
                     /translation="GGVTLKTVRQIARSGVDRISVGRLTHSAPALDMSLEVTRIR"
BASE COUNT          492 a          813 c          686 g          442 t
ORIGIN      
        1 gccgccggag ccgccttttt gtcgacggcc gccacgtggt cccgcagcac gggcagcaag
       61 accgtcacgc tggtgccctg tccgaggcct ttggattcga cccacacacg tccgctgtgg
      121 gcctccacca cctcgcggca gaacgccagg cccaatccca tcccgccgta aggccgcgtg
      181 agggtcttgt ccacatggaa gaacttctgg aacacgtaag gcaacgcctc cgacggaatt
      241 ccacgtcccg tgtccctcac ccggaactgc gcctggccgt tttcgatgct cttcgtttcg
      301 acgaacacct tgccctcccg cggcgtaaac ttcagcgcat tggaaacgag gtgttcgaag
      361 accttggaga ggctgtccgg gtcggcctga acgaagagcg gctcctcagt attctcaaaa
      421 atcagctgca cccccttctt gtcgaaaagg gtctgcatgg tggtggcctg tttgatggcc
      481 aactcccgca gggagatcgg cttcatggcc aacatcgccc ccccctgcga gagggcacgg
      541 atatccaaca actgattgat gagcttgacc agcaggtggg attgctccaa gatcttcttg
      601 agactctgcg cctgcttctc ttccagcgtc cccatgcggc cggtgaggag cacctcgctc
      661 aatcccagga tggacgtcaa cggcgtgcgg agctcgtggg agatggtcgc caggaaatcg
      721 tccttcatct gattgatctg caccagttcc gcattttccc ggcggaggcg gaaggaatcc
      781 atcagccgct gaatcaaccc cgccaggtcc agggtgagca gatcgtcctt catgaggaag
      841 tcgagaatcc cccgcttcat cgcctccacg gccatgcgcg cgttgttggt tccccccatc
      901 atcaccagcg ggagaggcgg cgtggtctgt cccacgcggg aaaggacttc gagggaatcc
      961 atatccgcca ggttcatgtc caggaaaatg aggtcgtagg actgacggta gatgcggtcc
     1021 agcatctccg cgccggtcgg acagatgtcc accatgaccg tatacaccga cagttgtttg
     1081 aaccgctcgg cgaaggcctt gacaaaggcg tggtcgccgt cgatgatgaa gactctcagt
     1141 gccccgactt tagtcgacat tcctgttttc ccctcaaaaa acgccgtgtc cgaaaaaatc
     1201 cgacttccca aaaacctttg gaatggaaac gtcctcccca aaaacacttc cgaccggctc
     1261 caagcacgga cgttttagta tatagtccgt cgtaacccaa gtcaagcccg ttcgtctcct
     1321 tttttcagcc gtatcgcgcc ttcgccgaaa cggcgcaaac cggccgctgt ccgcagaagg
     1381 agccgcccct cgccgtcgat gccttccact cgccccgcca cagtcccttg ggccgtttca
     1441 caaacgaccg tccggccttt catcgaaagg cactcctcac agtcccggcg aaacgccgaa
     1501 aaaccatccc gttgatagcg tcgaagactc ccggcgaagc gtttcagcca atcctccaac
     1561 agcgcctgcc gatagaacgg tttcccggtc atttcacgaa gggaggcggc gggcggcgtc
     1621 atcccccggg gagagggatt attgatgttc acccccacgc cgagaacgac ccagtgcacg
     1681 cggtccatct ccgacgacat ctccgtcaaa atgccggcca ccttgcggtt gtcgatccaa
     1741 acatcgttcg gccatttcac gcaagccgga agccccgtcg cgcgccgcaa aaccatcgcc
     1801 caatccaacg cggccaccag cgccagcgcc ggcacctgcg ccggagggat cgccgggcgc
     1861 aaaagaagag agaaccacaa cccccccctg ggcgaaagcc accggcgccc cattcgcccc
     1921 cgaccggagg tctgctcgtc cgccaagaca agcgtgcctt ggggcgcgcc ggccagggcg
     1981 cgctccttca attcgtcctg cgtcgaaccg agggatggga aatggcagat cggtcgcccc
     2041 aacgaggcgg cgcgaaaacg gtccgtgtcc agcgcctgcg gggaattttc caaccggtat
     2101 cctttccggt tcgtccccgt gatgtcgtaa ccccattccc tcaagcggac cacctgttta
     2161 tggatggcgg cgcgcgtcaa gcacaacccg gcggccagac ggctgccgga gacatagcgg
     2221 ccccgggcgt tccgcagcac tgtcaaaact tctttttgtg ggaggaggcg tcgtcggaaa
     2281 ggcgaggatc cgcgacgaga cggcgtcatc ggatgcgggt cacttccaaa ctcatatcga
     2341 gggcgggggc ggaatgagtc aaccggccga cggagatgcg gtccacgccc gagcgggcga
     2401 tctggcgcac cgtcttcaag gtaacgccgc cgg
//
LOCUS       DAOTFD010000309         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_36210_length_2413_cov_2.651399, whole genome shotgun sequence.
ACCESSION   DAOTFD010000309 DAOTFD010000000
VERSION     DAOTFD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36210_length_2413_cov_2.651399"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="P5079_11570"
     CDS             complement(<1..307)
                     /locus_tag="P5079_11570"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY30660.1"
                     /translation="MSAPDLNATSTEEERNARHKARMERKKAVVDAAIAAATDTRGLL
                     LVNTGNGKGKSSAAFGVLARAIGHGMKVGVVQFVKGRSDTGEEAFFRRQPEVSWHVMG
                     "
     gene            complement(337..>2413)
                     /locus_tag="P5079_11575"
     CDS             complement(337..>2413)
                     /locus_tag="P5079_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY30661.1"
                     /translation="TVIMVEKPPEPEFGYREDRDATRSTKPKEQRFVHLATVAEIKDT
                     FKEKSSYSRYNGRENVSISVQKQADANTIVVAKRVREAMEELSGSLPKTLKVELVYDE
                     SIFITTAVNGVFTDGITGGVLAFLVLYFFLKSFGDALIVSIAIPISTMIIFISMYFKG
                     ISINMLSLAGLGLAIGNLVDNSIVVVENTSRLRKEGGDVVRGAIEGAEEVGNSMVTSA
                     LTNVAVILPLLFAKGVAQQLFMDLFFASVGASIVSMIVSLTLIPRLAAHPLALPPFLL
                     FWKKKKKEEPSFVEPPTPSEHLPLGGEEAPPPGRAVLGPLGAAYRFFKRGLTEEAFAG
                     IIERYQTLLNWILNNPKNTSLIIAGLLLFSMGVLAIHEKVFMPKFDQGQFMLRIDMPV
                     GTRLEVTNRVSERIEKIIQEIPGVKDQTTNVGSSSTESIEALGSHQAQCIINIDREKQ
                     KRSTEDVLAHLKRALEKTNLEGAEVQYILQDSILSTAFEPSAPIVVEIKGPDLVTLKK
                     ISTDITAELEKVRGIYGIKSSFALPSSETRISIDKDRASGYQLSVSEIARTALAAMGL
                     AGMETREVQTLSGGERQRAAIATLLAQQPRLYLLDEPLAHLDLHHQVAVLDHFRSLAA
                     SGAGVAMVLHDINLTARYASHVLLLDGEGGHVIGPVAEVLTTARLSAAFGHPLRAIAD
                     GEHTLFVPV"
BASE COUNT          394 a          663 c          851 g          505 t
ORIGIN      
        1 cgcccatgac gtgccagctc acctcgggct ggcggcggaa gaaggcttcc tcgccggtgt
       61 cggagcggcc tttgacgaat tgcaccacgc ccaccttcat gccgtggccg atggcccggg
      121 cgagcacacc aaaggcggcg ctggacttgc ccttgccgtt gccggtgttg accagcagca
      181 ggccgcgggt gtcggtggcg gcggcgatgg cggcgtcaac gacggccttc ttgcgttcca
      241 tgcgggcctt gtggcgggcg ttgcgttctt cctcggtgga cgttgcgttc aggtcgggtg
      301 cgctcatggc ttctctcggg ggtggttgtg gccggttcag accggcacga acagggtgtg
      361 ttcgccatcg gcgatggccc gcagggggtg gccgaaggcg gcagacaggc gcgcggtggt
      421 gagcacctcc gcaaccgggc cgatcacgtg gccgccttca ccgtcgagca gcagcacatg
      481 gctggcgtag cgggcggtga ggttgatgtc atggaggacc atggccacgc cggcgccgct
      541 ggcggccagg ctgcggaagt gatcgagcac ggcgacctgg tggtgcagat ccaggtgggc
      601 caggggttcg tcgagcaggt agaggcgcgg ctgctgggcg agcagcgtgg cgatggcggc
      661 gcgctggcgt tcgccgccgg agagcgtctg cacctcgcgc gtctccatgc cggccagtcc
      721 catcgccgcg agggcggtgc gggcgatttc ggagacggaa agctgatatc cggaagcccg
      781 gtccttgtcg atggaaatgc gtgtttcgga ggacggcaag gcgaagctgc ttttgatgcc
      841 gtaaatgccg cggacttttt ccagttcggc ggtgatgtcc gtggagatct ttttcagggt
      901 gacgaggtcg ggtcccttga tctccaccac aatgggcgcg gagggctcga acgcggtgga
      961 gaggatgctg tcctggagga tgtactgcac ctcggcgcct tccaggttgg ttttttccag
     1021 ggctcgtttt aggtgggcga gcacgtcttc cgtggaacgt ttttgttttt cccggtcgat
     1081 gttgatgatg cattgggcct gatgcgaacc gagggcctcg atggattccg tgctgctgga
     1141 accgacgttg gtcgtctggt ccttgacccc cgggatctcc tggatgattt tttctatccg
     1201 ttccgacacg cggttggtca cttcgagcct tgtccccacg ggcatgtcga tgcggagcat
     1261 gaactgtccc tgatcgaact tgggcataaa gaccttttcg tgaatggcga gaacccccat
     1321 gctgaacagc aggagccccg cgatgatgag agaggtgttc ttggggttgt tcaggatcca
     1381 gttcaacagg gtttgatagc gttcgatgat tcccgcaaag gcctcttccg ttaggcccct
     1441 cttgaaaaaa cggtaggcgg ctcccagggg tccgaggacg gctcttcccg gaggcggcgc
     1501 ttcttctccc cccaggggga gatgttccga gggcgtcggc ggctcgacga aagagggttc
     1561 ttcctttttt ttctttttcc agaagagcaa gaacggcgga agagcgaggg ggtgggcggc
     1621 caggcgcggg atgagggtca gggagacgat catggacacg atggaggcgc cgacggaggc
     1681 gaagaagagg tccatgaaga gctgttgggc cacgcccttg gcgaagagga gcggcaggat
     1741 gacggccacg ttggtgaggg cggaggtgac catggaattg ccgacctctt cggccccctc
     1801 gatggcgccg cgtaccacgt cccccccctc cttgcgcagg cgggaggtgt tttcgaccac
     1861 cacgatggag ttgtccacca ggttgccgat ggccagtccc aggcccgcca gcgagagcat
     1921 gttgatgctg atgcccttga aatacatgga gatgaagatg atcatggtgg aaatggggat
     1981 ggcgatgctg acgatgaggg cgtccccgaa acttttgagg aagaagtaga gcaccaagaa
     2041 ggccagcact cccccggtga tgccgtcggt gaagacgccg ttcaccgccg tcgtgatgaa
     2101 gatggattcg tcgtagacca gctccacctt gagcgtcttg gggaggctcc cggaaagttc
     2161 ttccatggcc tcccgcacgc gcttggccac cacgatggtg ttggcgtccg cctgtttctg
     2221 cacggagatg gagacgtttt cccggccgtt gtagcgcgag tagctgcttt tctccttgaa
     2281 agtgtccttg atctcggcga cggtggccag gtggacgaag cgctgttcct tgggtttggt
     2341 ggagcgtgtg gcgtcgcggt cttcgcggta gccgaactcg ggctccggcg gtttttccac
     2401 catgatcacc gtc
//
LOCUS       DAOTFD010000310         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_36573_length_2398_cov_4.597951, whole genome shotgun sequence.
ACCESSION   DAOTFD010000310 DAOTFD010000000
VERSION     DAOTFD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36573_length_2398_cov_4.597951"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /locus_tag="P5079_11580"
     CDS             complement(<1..2341)
                     /locus_tag="P5079_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30662.1"
                     /translation="MQGLADEWTALKETFKKALEEKEKALLNLRLSQQETRLSMELRL
                     QAAEEEKKKITVLMKERLEVLDKRHAYERVHFEELLKAQQSAHQKVAAEWEMIQAQVK
                     GERERRRKELDAAVHKAQQELQGMKARQEQEREAWEQALREKNAEIQAVHDAIAAAEK
                     DGAAEWERMQVSFQETKKNWEKEVLEKEEELRLFQTEQAMVLQGKEKELAELKASWDE
                     RIRRHQSQWEADIAEMRDQKAEIEKNIAEVERAYADKKAAFQAEVQQKDQRRGDEQTQ
                     LTFKQAEWKRTLQKLESDHQALLKPLNDKLAALEWRRQAAESLFKKNVEEKERQIQSL
                     QAKMARQEATSLARWEEQATLLSTDIQRLREQAAEKTQKAKQEIDRLSQLVAQRDAEL
                     ARLQAEGDEQLKRWTAERRELERAQEKELAEARAQIEAATQEVALRKQADEKKLQETE
                     EILAQWKARAEEQVLRLEEEGRKRSENWEADRKALADRFEAEEKALAEARAEMERRAK
                     EAEDARAAAAEGLEEGRKRISDEKSALEARTRQERVPLETRRTELVDSLAKAQQELEA
                     HLAALSAEKQRLEDAFEQKQKELQRSTKQQESEYSTTQQARRSALGKFLADARQVRAQ
                     YDEKMRVLSETIENIKREEAHLAASWAEEKKSLTAAAAQKKSVLESEISVLAGKELSL
                     WTALTETKKTSAAELEDLKQRLRGLDTGLAEQQRAWKEKLARLEQRFAQRKSRIGRVL
                     QEKKTEQVSTRHVKEEELNRLSEEVRKKKVDWEKAYKTLA"
BASE COUNT          304 a          884 c          576 g          634 t
ORIGIN      
        1 ccgccagcgt tttatacgcc ttttcccagt cgaccttttt cttccggact tcctcgctca
       61 gccggttcaa ctcctcttct ttgacatgac gggtggaaac ctgttccgtt ttcttttcct
      121 gcaacacccg gccgatccgg ctcttccgct gcgcaaatct ctgctccagc cgggccaact
      181 tctctttcca ggcacgttgc tgctcggcga ggccggtgtc cagtccccgc agacgctgtt
      241 tcagatcctc caattcggcg gcggaggtct tcttcgtttc cgtcaacgcc gtccacaacg
      301 aaagttcctt ccccgccaac acgctgattt cgctttccag aaccgacttc ttctgcgccg
      361 cggcggcggt caaactcttc ttttcctccg cccacgacgc ggcgagatgc gcttcctccc
      421 gtttgatatt ctcaatcgtc tccgacaaca cccgcatctt ttcatcgtac tgcgcccgga
      481 cctggcgggc atccgccaag aattttccca gggcggaccg gcgcgcttgc tgcgtcgtcg
      541 aatattcgga ttcctgttgt ttggtggacc gctgaagttc cttctgtttc tgctcgaacg
      601 cgtcctccag gcgctgtttt tccgccgaca gcgccgccaa atgcgcctcc aattcctgtt
      661 gcgccttcgc caaggaatcc accaactccg ttcgacgcgt ttccaatgga acgcgttcct
      721 ggcgggtccg cgcctccaac gcgctttttt catccgaaat cctcttccgg ccctcctcca
      781 gcccttcggc ggcggcggcg cgggcgtctt ccgcctcttt cgccctcctc tccatctcgg
      841 cccgcgcctc ggccagcgcc ttttcttccg cctcaaaacg gtccgccaac gccttccgat
      901 cggcctccca gttctccgac cgtttccgcc cctcctcctc cagacggagc acctgttcct
      961 cggcccgcgc cttccactga gccaggatct cttcggtttc ctgaagtttt ttctcgtcgg
     1021 cctgtttccg cagcgccact tcctgtgtgg ccgcctcgat ctgagcgcgc gcctccgcca
     1081 gttccttttc ctgcgccctt tccaattcgc ggcgctccgc cgtccatcgc ttcagctgct
     1141 catcgccctc ggcctgcaga cgggccagct ccgcgtcccg ctgggcaacc aactgggaaa
     1201 ggcggtcgat ctcctgcttc gccttctgcg tcttctccgc ggcctgctcc cgcaggcgct
     1261 ggatgtccgt cgacaacaac gtcgcctgct cctcccagcg ggcgagggag gtcgcctcct
     1321 ggcgggccat cttggcctgc aaactctgga tctgccgctc cttctcctcc acgttctttt
     1381 tgaacagcga ctccgcggcc tggcgccgcc actccagggc ggccagtttg tcgttcagcg
     1441 gtttcaggag cgcttggtgg tcggactcca gtttttggag agtcctcttc cattccgcct
     1501 gtttaaacgt gagctgggtc tgctcgtcac cccgtcgctg gtctttctgc tggacttccg
     1561 cctgaaaagc ggctttttta tcggcgtagg cgcgttccac ctccgcgatg ttcttctcga
     1621 tctccgcctt ctggtcgcgc atctccgcga tgtccgcttc ccactgagac tgatgccggc
     1681 ggatccgctc atcccacgag gccttcaact ccgccagttc cttctccttg ccttgcagaa
     1741 ccatcgcttg ttccgtttga aacaggcgca gctcctcctc cttttcgagg acttcttttt
     1801 cccagttttt cttggtctcc tggaaagaga cctgcatccg ttcccattcc gcggcgccgt
     1861 ctttctccgc ggcggcgatg gcgtcatgga ccgcctggat ctcggcgttc ttttcccgca
     1921 gggcctgctc ccacgcctcc cgttcctgtt cctggcgggc cttcatcccc tgcaattcct
     1981 gctgggcctt gtgcacggcc gcgtccagtt ccttgcggcg acgttcccgt tcccccttga
     2041 cctgggcctg gatcatctcc cattcggccg cgaccttttg atgggcggac tgctgagcct
     2101 tcaacagttc ctcgaaatga acccgttcat acgcatgccg cttgtcgagc acctccagcc
     2161 gctccttcat caacaccgtg atcttctttt tctcctcttc cgcggcctgc aggcgcagtt
     2221 ccatggacaa ccgggtctcc tgctgtgaaa gccggagatt aagcagcgcc ttttcctttt
     2281 cctccaaggc ctttttaaag gtctccttca gggccgtcca ttcatcggca aggccttgca
     2341 tctcccgttc gaattcctcc tgttgcacgg agcgcagtcg acggtttttc tcttcctc
//
LOCUS       DAOTFD010000311         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_37596_length_2358_cov_3.041251, whole genome shotgun sequence.
ACCESSION   DAOTFD010000311 DAOTFD010000000
VERSION     DAOTFD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37596_length_2358_cov_3.041251"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1130
                     /locus_tag="P5079_11585"
     CDS             207..1130
                     /locus_tag="P5079_11585"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30663.1"
                     /translation="MAHAAGTTSAAFLRLGTGARAAAMGDTFSSVSDDASATYWNPAG
                     MASLSKRELLAEHAVHVQDIAIERLAYVQPLERGGGFGPALGVSAAYLSLDGIEARTG
                     NTAQADRVFGASDFSAGFSYAHPLSRRPGTVSSLGVTAKFIQQKIDDRQANAYAADVG
                     FFQRFRRFQAGMVVTNMGSPVQFLEESYPLPRTFRLSLSYLPRTIPLLGSAGVESVRG
                     DSALAYRFGTEYRFGEMLAFRLGYAARSGSTQRALRGAGLGTVSDSEFARFTGLVGGM
                     GFRFFGYGLDYAFVPYGELGNSHKVTLSARF"
     gene            1144..>2358
                     /locus_tag="P5079_11590"
     CDS             1144..>2358
                     /locus_tag="P5079_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30664.1"
                     /translation="MPRIKSLFLTLGTGLFLSVGFSGAAFAVPQAVTDLVAYESGQSN
                     KIRLVWTTPQADVLQANSTYYIQYTTMTPGATTWAYTNAQVLISTSGVAVGTTVSFLI
                     TPPMNAQYSFHIWAANSNDPGNVSPMDTTASTATAFNSPFNLSVVGSGTEDWGMYGSL
                     AIDKFNYLHIAHLRNSYYDFYYSKWMGSWSSMQAEPTYNTGYYTSIAIDPLGSPCVVH
                     QDGNNYDLFFSSYNGSVWSNTNIESSGNTGYYASLAWMAVGGPLISYVDYSNSHLKVA
                     YQGGSGIWETEVVSTGSFAGTSVGLDGAQVPHIAYVDSANADLKYATWAGSWSTTTVD
                     ADRVQGGAGYNDVSLVFDGNGNAHMAYRANNESGFSMLKYARWTGTGWSTAAVDGGAL
                     NCQAGYYPSITLD"
BASE COUNT          441 a          676 c          723 g          518 t
ORIGIN      
        1 gattattttg aaaatgtgac aaaaaacaca ttttaaagcg attgacaatg taacttggtc
       61 cttctatact tagaatactt agtaaactaa tatagccccc agacatgccg agaccgagcc
      121 gagcaggtat caatacccta cggtggcgga cagggacgat gagaacgtcc tgtctgtcta
      181 ttttttttat attttcaata ggaatcatgg cgcacgccgc gggaaccact tccgcggctt
      241 ttttgcgttt gggaaccggc gcccgcgccg ccgccatggg agacaccttc agttccgttt
      301 cggacgacgc ctccgccact tattggaacc ccgcgggcat ggcctctttg tcgaaacggg
      361 aactcctcgc cgagcatgcc gtgcatgtgc aggacatcgc cattgaacgg ctggcctatg
      421 tgcagccgtt ggagagagga gggggattcg gtcccgccct gggtgtttca gcggcgtatc
      481 tgagtctgga cggtatcgag gcgcgcaccg gcaataccgc ccaggcggac cgcgttttcg
      541 gcgccagtga tttttccgcc ggtttttcct atgctcaccc cctctcccgc cgccctggaa
      601 ccgtttccag tctgggcgtc accgccaaat tcatccagca gaagatagac gaccgccaag
      661 ccaacgccta cgccgccgac gtgggctttt tccagcgatt ccgtcggttt caggccggga
      721 tggtcgtgac caacatgggc tccccggtcc aatttttgga ggagtcctac cccttgccga
      781 ggactttccg gctgagtctg tcctatctgc cgcggacgat tcccctgttg gggtcggccg
      841 gagtggagag cgtccggggg gattcggcgt tggcgtatcg gttcggcacg gagtatcggt
      901 tcggcgagat gttggcgttc cggttggggt atgcggcgcg atccggatcc actcagcgcg
      961 ccctgcgcgg ggcgggcctc ggcacggtgt ccgacagcga attcgcccga ttcacgggcc
     1021 tggtgggcgg gatgggtttc cgtttcttcg ggtacggttt ggactatgct tttgtgccct
     1081 acggcgagct gggcaattcg cacaaagtca cgttgagcgc gcggttttga ttccatgaac
     1141 aggatgccga gaatcaaatc gttgtttctg acgctgggga cggggctctt tttgtcggtg
     1201 ggcttttcgg gggccgcgtt cgccgtgccg caggcggtga cggatttggt ggcgtacgag
     1261 tccggccaat ccaacaagat ccggctggtt tggaccacgc cccaggcgga cgtccttcag
     1321 gccaactcga cgtattacat ccagtacacc accatgacgc ccggcgccac cacctgggcc
     1381 tacaccaacg cgcaggtgct gatctccacc agcggcgtgg cggtgggcac gacggtgtct
     1441 ttcctcataa cgccgcccat gaacgcgcaa tacagtttcc atatttgggc ggcgaactcg
     1501 aacgatcccg gcaacgtttc tcccatggac accacggcct ccacggcgac ggcgttcaac
     1561 tcacccttta acctgtcggt cgtcggctcc gggacggagg attgggggat gtacggctcg
     1621 ctggccattg acaagttcaa ttatctgcac atcgcccatc tgcgcaacag ttattacgac
     1681 ttctactatt cgaagtggat ggggagttgg agttcgatgc aggccgaacc cacgtataac
     1741 acggggtatt atacgtccat cgccatcgat ccgctgggga gcccgtgcgt ggtccatcag
     1801 gacggcaaca attacgacct gtttttctcc tcctataacg gttccgtgtg gagcaacacc
     1861 aacatcgagt cctccgggaa cacgggatac tacgcgtccc tggcctggat ggccgtcggc
     1921 ggaccgctta tttcctatgt ggactactcg aacagccatt taaaagtggc gtatcagggg
     1981 ggcagcggga tttgggagac ggaagtggtg tccaccggtt ctttcgccgg cacgagcgtg
     2041 gggttggacg gggcgcaggt cccgcacatc gcctatgtgg acagcgccaa cgcggatttg
     2101 aaatacgcca cgtgggcggg ctcatggagc acgacgacgg tggatgcgga cagggtgcag
     2161 ggcggggcgg gatacaatga cgtttcgctg gtttttgacg gcaacgggaa tgcccacatg
     2221 gcttatcgtg ccaacaatga aagcggtttt tccatgctga aatacgcccg gtggaccgga
     2281 acgggctggt ccacggccgc cgtggacggc ggcgccctga actgtcaggc ggggtattat
     2341 ccgtccatca cgctggac
//
LOCUS       DAOTFD010000312         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_37649_length_2356_cov_4.510213, whole genome shotgun sequence.
ACCESSION   DAOTFD010000312 DAOTFD010000000
VERSION     DAOTFD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37649_length_2356_cov_4.510213"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="P5079_11595"
     CDS             complement(<1..727)
                     /locus_tag="P5079_11595"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30665.1"
                     /translation="MLNDPVQFLKGVGPKRALYLGELGIKTVWDLLTHFPREHEDRSE
                     KIPIAHVQPGTFKTLRGRVEGFEVIPVGRSLGLGKALLFDGTGYIWAIWFRRTSPQYD
                     AFETLTKKLVKGTQLLVHGHIERNHTDLQVRVDEYEVLSQEHPAPTIHVDRLVPIYPL
                     TEGLDARWLRELIWTALNEHLAEAKDYLPGPFLEKYRLAPLAWAVQKLHFPADWAERD
                     RARRRLAFEDFFFLELALALSRER"
     gene            complement(770..1591)
                     /locus_tag="P5079_11600"
     CDS             complement(770..1591)
                     /locus_tag="P5079_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30666.1"
                     /translation="MKKSLVRELLLITLLLAGFPLPAEELDLAKWEKGVFSQNGEDGV
                     IEKIFELIPPSTRYAVEFGAGDGVNLSNTRNLVLHGWSALMIEGVPRKAEKCKRNYRD
                     QPRVAVVQAWVYPGNVELLFEENNVPVDLDLLSIDIDSNDYYVWRVINKYRPKVVLIE
                     YNPYFAPPKKAVIKFHPMNYYDDNSCYWGASIQSLYELGKAKGYELVHCTSRGSNLFF
                     VDKKYFERFKLADNSPAKLYRPQKDFTPETAPTPTGEDLVVPEFRIKKEWIFDRY"
     gene            1674..1865
                     /gene="rpmB"
                     /locus_tag="P5079_11605"
     CDS             1674..1865
                     /gene="rpmB"
                     /locus_tag="P5079_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019127135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRY30667.1"
                     /translation="MAYRCVVCGKGPAAGKTVSHSHRGTNRRFLPNLQRIKVQLPGGP
                     QRQYVCTKCLKSDRVRKAS"
     gene            complement(1866..>2356)
                     /locus_tag="P5079_11610"
     CDS             complement(1866..>2356)
                     /locus_tag="P5079_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30668.1"
                     /translation="DYMAYGVAFFNIPGIMYWDRYKPDQLPVRMRGGLSFTPVPIFSF
                     LTEYDKRFYRNGLSQPGQIHLGMELTLATWLQLRGGTYGEDLSDSEKTAYSAGFSVAS
                     SQKHQLDFALRTYRLAGERVYNYFVSIIFPLPRSSTEGRYVTSDSNRRASLRPEPLEE
                     RF"
BASE COUNT          444 a          640 c          764 g          508 t
ORIGIN      
        1 ggcgttcccg cgagagcgcc agcgccagtt ccaggaagaa aaaatcctcg aaggcgagcc
       61 gtcggcgggc ccggtcgcgt tcggcccagt cggcgggaaa atggagtttt tgcacggccc
      121 aggccagcgg cgcgagccgg tacttttcca ggaacgggcc gggcaggtaa tcctttgctt
      181 cggcgaggtg ctcgttcagc gccgtccaga tcagttccct caaccatcgc gcgtcgagcc
      241 cttcggtgag cggatagatg gggacgagac ggtccacatg gatggtgggg gcggggtgct
      301 cctgggagag gacttcgtat tcgtccaccc gcacctgcag gtccgtgtgg ttccgttcga
      361 tgtggccgtg caccaggagt tgggtcccct tgaccagttt tttcgtgagg gtctcgaagg
      421 cgtcgtactg cggcgaggtg cggcggaacc agatggccca gatgtatccg gtgccgtcga
      481 agaggagggc cttgcccagg ccgaggctgc ggcccaccgg gatgacctcg aagccctcca
      541 cgcgtccgcg cagggtcttg aaggtgccgg gctggacgtg ggcgatgggg atcttttccg
      601 agcggtcctc atgctcgcgc gggaaatggg tgaggaggtc ccagacggtc ttgatgccga
      661 gttcgccgag gtagagggcg cgtttcgggc cgaccccttt caggaattgg acggggtcat
      721 tcagcatggg gagcggccgt cgcgggcgcg cccgtcggcg ccggtcggtt taatagcggt
      781 cgaatatcca ttctttcttg atgcggaact ccggaacgac gaggtcctcg cccgtgggtg
      841 tcggcgccgt ctccggcgtg aagtccttct gcggccgata gagtttggcc ggggagttgt
      901 ccgccagttt gaacctttcg aaatatttct tatcgacgaa aaaaaggttg gatccccggc
      961 ttgtgcagtg gacaagctca tagcctttgg ccttcccgag ttcgtaaagg gattggatgc
     1021 tcgcccccca gtagcaggag ttgtcgtcgt aataattcat gggatggaat ttgatcaccg
     1081 cttttttggg aggggcaaaa taggggttgt attcgatgag gacgaccttc ggccggtatt
     1141 tgttgatgac cctccagacg taatagtcgt tggaatcgat gtcgatggaa agcaggtcga
     1201 gatcgacggg gacgttgttc tcttcgaaga gcagttcgac gttcccggga tagacccagg
     1261 cctgcaccac cgcgacccgc ggctgatcgc ggtagttgcg tttgcatttc tccgcttttc
     1321 tgggaacccc ttcgatcatg agggcgctcc agccgtgcag cacgaggttc cgggtgttgc
     1381 tcaggttgac gccgtctccc gcgccgaact ccaccgcgta gcgtgtcgac ggcgggatga
     1441 gctcgaagat cttttcgatg acgccgtcct ccccgttttg agaaaacacg cccttttccc
     1501 atttcgccaa atcgagctct tcggcgggga gcgggaagcc ggcgaggagg agcgtgatca
     1561 gcaggagttc acggacaagg gattttttca tggaaagtct ttcggtttgc attccggagt
     1621 ccaatatgat atttcttgta gctcagtttt ggaaaagaca aaggagagat tttatggcgt
     1681 accgttgtgt cgtttgtgga aaagggcccg cggccgggaa gaccgtcagc cattcgcatc
     1741 gggggaccaa ccgtcgtttt ctgcccaacc ttcagcgcat aaaagtgcag cttccgggcg
     1801 gcccgcagcg acagtatgtc tgcaccaagt gcctgaagtc ggatcgggtt cgtaaggcca
     1861 gctgatcaaa accgttcttc gaggggttcg ggccggaggg acgctctccg gttcgagtcg
     1921 gaggtgacgt agcgtccttc cgtggacgat ctcggcagcg gaaagatgat ggaaacgaaa
     1981 tagttgtaga cccgttctcc cgccaatcga tacgttcgca gcgcaaaatc caactgatgt
     2041 ttctgtgaag aagcgacgga aaagccggcg ctgtaggcgg ttttttcgga atcgctcagg
     2101 tcctcgccgt aggtcccccc ccgcaactgc agccacgtcg ccagcgtcaa ctccatcccc
     2161 aggtggatct ggcccggctg cgacaggccg ttccggtaaa aacgtttatc gtattcggtg
     2221 agaaaagaga agatgggcac gggcgtgaag ctcagcccgc cgcgcatccg caccgggagc
     2281 tggtccggtt tgtaacggtc ccagtacatg attcccggaa tgttgaagaa ggccacccca
     2341 tacgccatgt agtccg
//
LOCUS       DAOTFD010000313         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_37698_length_2354_cov_2.829491, whole genome shotgun sequence.
ACCESSION   DAOTFD010000313 DAOTFD010000000
VERSION     DAOTFD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37698_length_2354_cov_2.829491"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2354
                     /locus_tag="P5079_11615"
     CDS             <1..>2354
                     /locus_tag="P5079_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30669.1"
                     /translation="GGAGDIVGWTDISPQPLNVTQYTADWAVNFSALQTGGTNYVSVR
                     TWKATGATATVTDAFFIRKDILVPGITDPAPGDDTVWRNANTGVYDVDFNDTPALNGS
                     NLSKFQVRASTSSGGTGPFSPDWTDVLTLNATYYTTNWSLPTAVFTGLKEGQNYVSLR
                     TYDGAGNTYSLTDAFFVRKDTTPPSITNNMGAGDTAWRRQNNGAYNVDFADTGGSQLA
                     RFEVKASTLPGGAGPFLFDFTTVVANINAASYTTDWALPGTLFNLLQGGTNYVSVRVF
                     DAAGSTTAAVDAFKILKDTAAPVVSDSQSGDDAWRNAAGAAYNVSFADALSRLDTLQY
                     AVWTGAGQTGTPVLSYTTIAGPNLGQPSYSTPWSVDFANLAAGTNYVSVRAWDTAGST
                     QTTVDVFYVKKDTSPPVVTDNQPGDELWRNADPGAAYNVDFQDPLSLLATVQYTVNSA
                     AGLPSGTGDVIAWTNIVAPPLNQASHTADWALNFSLLHEGTNYVSVRAHDTLSQGTTV
                     QDVFVVRKDTTPPTAQDNNSPNDTLTLSAPGTLYDVDFLDLGGAALDHAEYTVWTDPN
                     FAGGQILPYTDIILSINATYYIEDWPVNFNALPNHATSYVSVRIYDNAGNAAVSTDVF
                     KVIKEAQVPVISDNQSGDAVWRDQNDGTYDVDFQALSGQNLDKFQVRSSTKPGNAGPF
                     TSDWTDVATAIGDTLYTTNWSLPSTVFEAMLNDATNYVSVRIYDLVPSSNTQADVFYV
                     RKSTSGPTITDLQDGDTTYRSAAGTAYNVDFHDPGAGLQRADYT"
BASE COUNT          467 a          869 c          632 g          386 t
ORIGIN      
        1 cgggcggcgc gggggacatc gtcggatgga cggacatttc accccaaccc ctcaacgtga
       61 cccaatacac cgcggactgg gccgtcaatt tctccgcgtt gcagacgggc gggaccaact
      121 atgtttccgt gcggacctgg aaagccaccg gagccaccgc caccgtgacc gacgcctttt
      181 tcatccggaa agacatcctc gtgccgggca tcaccgatcc cgcgcccggg gacgacaccg
      241 tttggcgcaa cgccaacacg ggcgtttatg acgtggattt caacgacacc cccgccttga
      301 acggttcgaa tctttccaag ttccaggtcc gggccagcac ctcctccggc ggcacggggc
      361 ctttttcgcc cgattggacg gacgtgctca ctctgaacgc cacctactac acgacgaact
      421 ggtctctccc gaccgcggtg ttcaccggcc tgaaagaggg gcagaattac gtcagcctgc
      481 ggacgtacga cggggccggc aatacctact cattgaccga cgcctttttt gtccgcaagg
      541 acaccacgcc gccttccatc accaacaaca tgggtgccgg cgacacggcg tggcggcggc
      601 agaacaacgg cgcctacaac gtggatttcg ccgacaccgg cgggagccag ttggctcgtt
      661 tcgaagtcaa agcatccact cttcccggcg gcgccgggcc tttcctcttt gatttcacaa
      721 cggtcgttgc caacatcaac gccgcctcct atacgacgga ttgggctctc cccggaaccc
      781 tgttcaacct cctccagggc ggcaccaact acgtctcggt gcgcgtcttt gacgccgccg
      841 gatccaccac cgccgccgtc gacgccttta aaatcctcaa ggacaccgcg gcgcccgtgg
      901 tttcggacag ccagtcgggg gacgacgcct ggcggaacgc cgccggcgcc gcctacaacg
      961 tctccttcgc cgacgcgctg tcgcgcctgg acacgctgca atacgccgtc tggaccggcg
     1021 ccggtcaaac cggaacgccg gttttgagtt atacaaccat cgccggaccc aacctcggcc
     1081 aaccgtcgta ttcgacgccc tggtccgtgg atttcgccaa cctggcggcc ggaaccaatt
     1141 acgtctccgt gcgcgcctgg gacacggccg ggtccaccca aacgactgtc gacgtcttct
     1201 atgtgaaaaa agacacctct ccgcccgtgg tgacggacaa tcaaccgggc gacgaactct
     1261 ggcgcaacgc cgatcccggc gcggcgtata acgtggactt ccaggatccc ctctccctcc
     1321 tggccaccgt tcaatacacc gtgaacagcg cggccgggct gccgtccgga accggcgacg
     1381 tcatcgcctg gacgaacatc gtcgcgccgc cgctgaacca agcctcccac accgccgact
     1441 gggctctgaa tttctcgctg ttgcacgaag gcaccaacta cgtctccgta cgcgcccacg
     1501 ataccctctc ccaggggacg acggtgcagg acgtcttcgt ggtgcgcaaa gacaccaccc
     1561 cgcccacggc gcaggacaac aattcgccca acgacacgct gaccctctcc gccccgggaa
     1621 cgctgtatga cgtggacttc ctcgacctcg gcggcgccgc gctggaccac gccgagtaca
     1681 cggtctggac cgaccccaat ttcgccggag ggcagatcct gccctacacg gacatcatac
     1741 tctccatcaa cgccacctat tacatcgaag actggccggt gaacttcaac gccctgccca
     1801 accacgccac ctcctacgtg agcgtccgga tttatgacaa cgcggggaac gccgccgtgt
     1861 ccacagacgt cttcaaagtc atcaaagagg cccaggtccc cgtcatcagc gacaaccaat
     1921 ccggcgatgc cgtctggcgc gaccaaaacg acggcaccta cgacgtggat ttccaggcgc
     1981 tgtccggcca gaacctggac aagttccagg tccgttccag caccaaaccc ggcaacgccg
     2041 gaccgttcac ctcggactgg acggacgtgg ccaccgccat cggcgacacc ctctacacga
     2101 cgaactggtc cctgccgtcg acggtgttcg aggccatgct caacgacgcc accaactacg
     2161 tgtccgtgcg gatctacgac ctcgtgccgt ccagcaacac ccaggcggac gtgttctacg
     2221 tgcgcaaatc cacctccggc ccgaccatca cggacctcca ggacggcgac accacttacc
     2281 ggtccgccgc cggaacggcc tacaacgtgg acttccacga tcccggcgcc ggactccaac
     2341 gggcggacta cacg
//
LOCUS       DAOTFD010000314         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_38661_length_2317_cov_2.997347, whole genome shotgun sequence.
ACCESSION   DAOTFD010000314 DAOTFD010000000
VERSION     DAOTFD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38661_length_2317_cov_2.997347"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2317
                     /locus_tag="P5079_11620"
     CDS             <1..>2317
                     /locus_tag="P5079_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRY30670.1"
                     /translation="QVRKIMSHPVVDSGYDNVNHNYRIGSLYLQKRRYRSLLFTLAHE
                     MGHNVGMELDSPRRLFRSPGVNEWSADVFAAAFFERIGQETDWIFDEEYETICDTCKK
                     GPARSGFLRFFRQHNAAREVNKFLIRQFREYRSRIPWTWSHLAQAILAEAARSAEKPP
                     LADGVVPDFDETVAALLIDSLEIALGRSGQLEKEALKAEIAKKFPRKIVPRSIDANDH
                     DGIDYVIPPYELLDRIFKNFIFKAGATVRWLMPLGLWLARKAPLKKFLGLETAPFGRT
                     RTLRIAVLQNHPDSGVYLLNYLENYRRANKVDMEIVVFQASRGELPDLSQFDGVMGSG
                     SPYNVDELDAEGAQWHKQEQALLRRAVEANKPVLGICFAHQNLAATFGGQVRRADHGL
                     YVGRMKVRLTDPDDPLFAGVPSEIWASEAHGREVPASGVPLGFKKIAESELTAVEGIR
                     HAQPDKKVYGLQFHPEVDLDVVDKTLSKGEMSNEHGRKILDNFLGIVRQEKGARLARL
                     EKEYGAKVAWWLEEAFISIATVVAMNVPALAVYFVGLQGLVRLAFFLSHFPGMRGPPT
                     LRNLYDQLGSALLVSLLSFLPYAFVPLGLDVSLATALYSLLGFVVHGLVNRGVFRGWR
                     FLPSRPASVGSSVPPPDAVEDRRTKLGVSGLFEGYFGEKPSDPIAGVFAEAILAGKYG
                     FENLSRYFQGSRQEKPASEEAARRISRKIEYIKLVRDAHRELLRTRQARLRSGGNIQF
                     HLPTALWPELTHELAEFFMEEDAGERGVSETL"
BASE COUNT          422 a          650 c          777 g          468 t
ORIGIN      
        1 caggtgagga agatcatgtc ccatccggtg gtggactcgg gctatgacaa cgtgaaccac
       61 aattaccgga tagggtctct ctatctgcag aagcgccgtt atcggagcct tctgttcacc
      121 ctggcgcacg aaatggggca caacgtcggc atggagctgg attctccccg gcggctcttc
      181 cgaagtcccg gggtgaacga atggtcggcg gacgttttcg cggcggcgtt tttcgagcga
      241 atcggccagg agacggactg gatattcgac gaggagtatg agaccatctg cgacacctgc
      301 aagaaaggtc cggcgcggtc gggatttttg aggttcttcc gccaacacaa cgcggcgcgc
      361 gaagtgaaca agttcctgat tcggcagttc cgggaatacc gttcgcgtat cccgtggact
      421 tggagccatt tggcccaggc cattctggcg gaggccgccc ggtccgccga gaagccgccg
      481 ttggcggacg gggtggtgcc ggatttcgac gagacggtgg cggcgcttct catcgacagc
      541 ctggagatcg ccctgggccg ctccggccag ctggagaagg aggccctgaa agcggagatc
      601 gctaagaaat tcccccggaa gatcgtgccg cgcagcatcg acgccaatga ccatgacggc
      661 attgattatg taattccacc ctacgagttg ctggacagga tcttcaagaa ttttattttc
      721 aaggcggggg ccaccgtgcg ttggctgatg ccgttgggat tgtggctggc ccggaaggcc
      781 ccgttgaaaa agttcctggg gctggagacc gctcctttcg gtcggacaag gacattgcgc
      841 atcgctgtgt tgcaaaacca cccggacagc ggggtgtatc tcctcaacta tttggagaat
      901 tatcgacggg cgaacaaggt cgatatggaa atcgtcgttt ttcaggcgtc ccgcggcgaa
      961 cttcccgacc tgtcgcagtt cgacggggtg atgggttccg gttctcccta caacgtggac
     1021 gagttggacg cggagggggc tcaatggcac aaacaggaac aggcgctcct gcgccgggcg
     1081 gtggaggcga ataagcccgt tctgggaatt tgtttcgcgc atcaaaatct ggcggcgacg
     1141 ttcggcgggc aggtgcgccg ggccgaccac ggtctctacg tcggccgcat gaaagtccgc
     1201 ctgaccgatc cggacgaccc gcttttcgcc ggagtcccgt cggaaatctg ggcttccgaa
     1261 gcgcatgggc gggaggtccc ggcgtccggc gtgccgctgg gattcaagaa gatcgccgag
     1321 tccgaactga cggctgtgga gggcatccgc cacgcccagc cggacaagaa agtgtatggc
     1381 ctgcagttcc atcccgaggt ggacctggac gtggtggaca agaccttgtc gaaaggcgag
     1441 atgtccaacg agcacgggcg aaaaatcctg gacaatttct tgggcatcgt tcggcaggag
     1501 aagggcgcgc gtcttgcgcg tttggaaaag gagtatgggg cgaaagtggc ctggtggttg
     1561 gaggaggcgt tcatctccat cgccacggtc gtggccatga acgttcccgc gctggccgtg
     1621 tatttcgtgg ggctgcaggg gctggtccgc ctggcgttct tcctgtcgca ttttcccgga
     1681 atgcgcggcc cgccgacgct gaggaacctc tacgaccaac tcgggtccgc cctcctcgtt
     1741 tccctgcttt cctttttgcc gtacgctttc gtcccgctgg ggctggacgt gtccctggcg
     1801 actgcgctgt attcccttct cggattcgtg gtgcacgggc tggtcaaccg gggcgttttc
     1861 cggggatggc ggttcctgcc gtcgcggccg gccagcgtgg gttcctcggt tccgccgccg
     1921 gacgccgtcg aggatcggcg gacgaagctc ggggtgagcg gtctttttga ggggtacttc
     1981 ggggagaagc cttccgaccc gatcgcgggc gtttttgcgg aggccatttt ggcggggaaa
     2041 tacgggttcg aaaacctgtc ccggtatttc cagggttcga ggcaggagaa gccagcctcc
     2101 gaggaagcgg cccggcggat atcccggaaa atcgaatata tcaaactcgt tcgcgacgcc
     2161 cacagggaac ttctccggac ccgtcaagcg cgcctgaggt cgggaggaaa catccagttt
     2221 catctgccca ccgccctgtg gccggagctg acccacgaac tggccgaatt tttcatggag
     2281 gaggacgccg gggagagagg ggtctcggag acgcttg
//
LOCUS       DAOTFD010000315         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_38930_length_2306_cov_4.316304, whole genome shotgun sequence.
ACCESSION   DAOTFD010000315 DAOTFD010000000
VERSION     DAOTFD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38930_length_2306_cov_4.316304"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="P5079_11625"
     CDS             complement(<1..634)
                     /locus_tag="P5079_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30671.1"
                     /translation="MPDKFGFFSKKNPDPSSGGVPVEPPTGASVLPADRSVLPGLQEL
                     EAALLQEETRSQEAFQKLLEEQKTLLEKIQESAAGSGTDRETFSQEIKTLQEETAAAL
                     HAWETRIAADREQWAKIVEEKSRELGEGAAKALWSQGESDLQTQQIQRLKEVHEKAER
                     ERREILARRLEQQRQAWRERLEKQQKESAALQARLEEIFATEAAYPLEIDR"
     gene            complement(677..>2306)
                     /locus_tag="P5079_11630"
     CDS             complement(677..>2306)
                     /locus_tag="P5079_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRY30672.1"
                     /translation="KVDDRKGLGDQDYILPWVPAPKTVNKENEVPKDAKMEAASGEIA
                     TASVRQTVRRFDVTVIHDDSVSKAQLSLVEEAIKSAYVRFEKVLRIILKPTKFAKYEM
                     STKIKEGFWDFLKPQYLLPGIVALLLFFFLFGPLLTFLRSFLKAMTARRSAEYNTETN
                     MRMEGKNEDQNKDELEGETENEEGEEDLENQEEEMKKFIPFSYVDETNLQRLTYLMSR
                     EPPEVIAIVVSYLKPEFVKEVLTPLSPELQAKVAMHMAAVKQTSEGEIRALDKEIKEK
                     IDYVVGGLQSLIQVLEQVDYRIRDNILEYLKNERPHLFEKVRKRILTFEDIANFPDAA
                     LQIVVRELRAENLARALKDMPPQMTEKFFNNMSKGAAALLKEEIEYSRPLTQDQMQEE
                     RQKILEVVKKLETDGKIAVREKSQEDFLEWEDMDLLSRFTATGQLGSAPAGAAAASAV
                     PQDPAKAQEYSAAGAQFYAEGKYAEALSYFEYAAQLGPALPEIQQYLGNTYYALGRVT
                     EALAAFERAVALNPQDQELKRWVDQFRAGVSTTA"
BASE COUNT          366 a          754 c          653 g          533 t
ORIGIN      
        1 gccggtctat ctccagggga taagcggctt cagtggcgaa gatctcctcc agccgggcct
       61 gcagggcggc gctctccttc tgctgcttct ccaatcgctc gcgccaggcc tgccgctgct
      121 gttccagccg acgggccagg atctcccgcc gctcccgctc cgctttttcg tggacctcct
      181 tgagccgctg gatctgttgg gtctgtaggt ccgactcgcc ctgagaccag agggccttgg
      241 ccgccccttc tcccaattcg cggcttttct cctcgacgat tttggcccac tgctcccggt
      301 cggcggcgat gcgggtctcc cacgcgtgca acgccgccgc ggtctcctcc tgaagggtct
      361 ttatctcttg ggaaaaagtc tcccggtccg tgccggaacc ggcggcggat tcctgtatct
      421 tctccaaaag ggttttctgt tcctccagga gcttttggaa cgcctcctgg gaacgggtct
      481 cctcctgaag cagggccgcc tccaactcct gcaagccggg caggacggat ctgtccgccg
      541 gaaggacaga cgcccccgtc ggcggctcga ccggtacgcc gcccgaggag gggtcgggat
      601 tctttttgga gaagaaaccg aatttatccg gcatgattcg tcgtcgcgtc ctctctcatg
      661 gtgtcgaaaa atcgcctcac gccgtcgtgg aaaccccggc gcggaactgg tccacccacc
      721 gtttcaattc ctggtcctgc ggattgagcg ccaccgcccg ttcaaaggcc gccaacgctt
      781 ccgtcacgcg ccccagcgcg tagtaagtgt tgcccagata ctgctggatc tccggcaggg
      841 ccggtccgag ctgagcggcg tattcgaaat aggacagggc ctcggcgtat ttgccctccg
      901 cgtagaactg ggccccggcg gccgaatatt cctgcgcctt ggcgggatcc tgcggaacgg
      961 ccgacgccgc ggccgcgccg gcgggggcgg aacccagctg ccccgtggcg gtgaaccggg
     1021 agagcaggtc catgtcctcc cattcgagga aatcctcctg ggacttctcc cggaccgcga
     1081 tcttgccgtc cgtctccagt tttttcacca cttccaggat tttctgccgt tcctcctgca
     1141 tctggtcctg cgtcagaggc cggctgtatt cgatctcttc cttcaggagg gcggccgcgc
     1201 ccttggacat gttgttgaag aacttctccg tcatctgggg cggcatgtcc ttcaaggcgc
     1261 gcgccaggtt ttccgcgcgc agttcccgca ccacgatctg cagggcggcg tcggggaaat
     1321 tggcgatgtc ctcgaacgtg aggattcgtt tccgcacctt ctcgaaaaga tgcggccgct
     1381 cgttcttcag atattcaagg atgttgtcgc gtatgcggta gtccacctgt tccagcacct
     1441 ggatgaggga ctgcaggccg cccaccacgt aatcgatctt ctccttgatc tccttgtcca
     1501 gcgcccggat ttccccctcg gaggtttgct tcaccgcggc catgtgcatg gctaccttcg
     1561 cctggagttc cggcgacagc ggcgtcaaca cctccttgac gaattcgggc ttcagatacg
     1621 acaccacgat ggcgatcacc tcgggcggtt cccggctcat cagataggtc aaccgctgaa
     1681 ggttggtctc gtccacgtag gaaaagggga tgaacttttt catttcttcc tcctggttct
     1741 ccaaatcttc ttcgccttct tcattttccg tctccccttc caattcgtct ttgttctggt
     1801 cctcgttttt cccttccatg cgcatgttgg tctcggtgtt gtattcggcg ctgcggcggg
     1861 ccgtcatggc cttcaggaac gaccgcagga acgtcaggag cggaccgaac aggaaaaaga
     1921 acaacaacag ggcgacgatc cccggcagga ggtattgggg cttgaggaaa tcccagaaac
     1981 cttccttgat cttcgtcgac atctcgtatt tggcgaattt cgtcggtttc agaatgatgc
     2041 gcaaaacctt ttcgaaccgg acgtaggcgg atttgatcgc ctcctccacc aacgaaagct
     2101 gggccttaga aacgctgtcg tcgtggatca ccgtcacgtc gaaccggcgc accgtctggc
     2161 gcaccgacgc ggtggcgatc tcccccgagg ccgcctccat cttggcgtcc ttcgggacct
     2221 cgttctcctt gttgaccgtc ttgggcgccg gcacccacgg caggatgtag tcctgatcgc
     2281 ccaatccttt gcggtcgtcc actttc
//
LOCUS       DAOTFD010000316         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_39274_length_2293_cov_4.923592, whole genome shotgun sequence.
ACCESSION   DAOTFD010000316 DAOTFD010000000
VERSION     DAOTFD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39274_length_2293_cov_4.923592"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="P5079_11635"
     CDS             complement(<1..592)
                     /locus_tag="P5079_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY30673.1"
                     /translation="MTPPPQTISVIVPAYNEREGLAHVLPRLTETLNSSGWTYEIILV
                     DDGSTDGGTDQPLPSGIKLLRSPIRRGYGNSLMWGIRSAQYPWIATLDADGTYPVEEL
                     PNLLPPTSAFDMVIGARTGAHYWKKQLHFPARLLFLWMAEFVCGEKIPDVNSGLRVFK
                     KTLLDKLPIVCPGFSFSTTLTISALSLHRPVHFVPIR"
     gene            797..>2293
                     /locus_tag="P5079_11640"
     CDS             797..>2293
                     /locus_tag="P5079_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30674.1"
                     /translation="MAKIEGLSPYSLKATRRGRCCLRAAATAVASVFVWVNVVWPAIP
                     PSEKGDKDFWSARRQKVVSSKKPSASPRLADGRPGWGAAELATPLSAVHPDPLINTGT
                     NLISSDPARWPAWVGQLPLAFIRLGELHSPAGRPDVVIVNDLHGQRQAQENIAAFLTE
                     LASKQPRGLTVAVEGAAGDIDFSPYRELGSDVANRRVAEAFLNRGWIQGVEMAGLTGP
                     SAVRYVGVEDPALYLANVEAYRRAVPLKGKMRRLLAEQKKTLDALKENLYTAAQRKAD
                     VLSKDYAEKRIDTGAYVDGLLAIDGQRGAARSPEIQKFVSARLLERRIDLSRAEADRA
                     ALVQRLSEVLPARSLEDWIQATLRYRLGQMEHGDYYRFVLELAARAGVEAKGFPHFYG
                     YARYCMLVGDVNRERFFDELVALEERVWRNLGATPEQAELRRLTRQADLVGKMADQGL
                     TDQEWRDYQVQREDIEKFDARLARLGAAVVEPFALDDLRTLEEFYVLAL"
BASE COUNT          439 a          593 c          786 g          475 t
ORIGIN      
        1 ggcggatggg cacaaaatgc accggtcggt gaaggctcag cgcggagatg gtgagcgtgg
       61 tcgagaagga gaatccgggg caaacgatgg gcagcttgtc caaaagcgtt tttttgaaga
      121 cgcgcaggcc ggagttgacg tcggggattt tctcgccgca gacgaactcc gccatccaga
      181 ggaacaagag ccgcgcgggg aagtggagct gtttcttcca gtaatgggcc cccgtccggg
      241 cgccgatcac catatcgaac gcggaagtcg gagggagcag gttcgggagc tcttcgacgg
      301 gataggtgcc gtccgcatcc agggtggcga tccagggata ttgggcggag cggatccccc
      361 acatcaggga gttgccgtac ccgcggcgga tgggggaacg gaggagtttg atcccggacg
      421 gcagaggttg gtcggtcccg ccgtcggtgg agccgtcgtc gacgagaatg atttcgtacg
      481 tccatccgga ggagttcagg gtttccgtca gtcgggggag gacgtgtgcc aaaccttccc
      541 gttcgttgta cgccggaacg atgacgctga tggtttgagg ggggggagtc atattttccg
      601 gccccatcat acattatttg agttgagcat ttcaaaaaag agccggcgga gcttttgttt
      661 gacaagggga cggaagaaag cgtaaattta caaatcgttt acaggctgtt aacggctttt
      721 gtggatgaaa aagtcactat tttagagtcg ttataactga aaatagttat aaatactctt
      781 tcttaaaata atgggaatgg ctaaaatcga aggattgtcc ccctattctc tcaaggcgac
      841 gcgtcgaggc cgctgttgtt tgcgtgcggc ggccacggcg gtcgcctctg tttttgtctg
      901 ggtcaacgtg gtctggccgg cgatccctcc gagcgagaag ggggataagg acttttggtc
      961 cgcccgccgc cagaaggtcg tttcctcaaa aaaaccatcc gcctccccgc gtcttgcgga
     1021 tggtcgtccc ggatggggcg ccgcggaact tgccacaccg ctgtcggcgg tgcatcccga
     1081 cccgctcatc aacaccggaa ccaatctcat ctcctcggat cccgcccgtt ggcccgcctg
     1141 ggtggggcag ttgcctctgg cgttcattcg tttgggcgag ctgcattctc ccgccggccg
     1201 gccggatgtc gtcatcgtta acgatctgca cggccagagg caggcccagg aaaacatcgc
     1261 cgcgttcctg accgagttgg ccagcaagca accgcgtggt ttaacggtgg ccgtggaggg
     1321 cgcggccgga gacatcgatt tcagccccta tcgggaactc gggtcggacg tcgcgaatcg
     1381 aagagtggcg gaggctttct tgaaccgcgg ctggatccaa ggcgtggaga tggcgggttt
     1441 gacgggccct tccgcggtgc gctatgtggg ggtggaggat cccgcgcttt atttggccaa
     1501 tgtggaggcg tatcgccggg cggttccgct gaaggggaaa atgcggaggc ttttggccga
     1561 acaaaaaaag acgctggacg ctctgaagga aaatctctac accgccgccc agcggaaggc
     1621 ggacgtcctc tccaaggatt acgcggaaaa acgtattgat acgggcgcct atgtggacgg
     1681 cctgttggcc atcgatggac aacggggggc ggcgcggtct ccggagatcc agaagtttgt
     1741 gtcggcccgg ctcctggaaa ggcgtatcga tctgtcccgc gcggaggccg atcgggcggc
     1801 gctggtgcag cgcctgtccg aggtcctgcc cgcccgctcg ctggaagatt ggatacaggc
     1861 gactttgcgc tatcgtctgg gacagatgga acacggcgat tattaccggt tcgttctgga
     1921 gttggcggcg cgcgccggtg ttgaggcgaa aggttttccg catttctacg ggtacgcccg
     1981 ctattgcatg ttggtggggg acgtgaaccg cgaacgcttt tttgacgaac tggtggcgtt
     2041 ggaggagcgg gtctggcgta acctgggggc cacgcccgag caggcggaac tccgccgctt
     2101 gacccgtcag gcggacctgg tgggaaaaat ggcggatcag gggttgaccg accaggagtg
     2161 gcgggactac caggttcagc gggaggatat tgaaaagttt gatgcgcgct tggcccgatt
     2221 gggggcggct gttgttgagc cgttcgccct cgacgatctg cggaccctgg aggaattcta
     2281 cgttctggcg ctg
//
LOCUS       DAOTFD010000317         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_39686_length_2279_cov_3.291367, whole genome shotgun sequence.
ACCESSION   DAOTFD010000317 DAOTFD010000000
VERSION     DAOTFD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39686_length_2279_cov_3.291367"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="P5079_11645"
     CDS             <1..1072
                     /locus_tag="P5079_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30675.1"
                     /translation="PAILLFTVVGIVYISLGGIKAVVWTNLVQALVILGGGIATLVFL
                     GSRITGGGATLWRTAAAGGRLHVLDYGVPFSAPDFWTSFFSNPNILWLAALNGLFGSM
                     AAFGTDHDLMQRLLTVETRRESQRTMALTIPVSLAVLLIYLSIGTGLYVFYAQNPALP
                     LPDNGDKIFPHFIAQVMPPLLRGLLLSAIVLASIDSPLASLSASFVTDLYRPFRRWLG
                     ERAGPGGRLGRLHRRLPPEDEAHFLRMSRWGVAGFGAVLAGLAFFFSHFENILWLAFK
                     IGGVTFGSLLGVFLLGLLTDRRGDRANVLAMTLSAGAMFVLLVLSEKHILPLGWSWLV
                     ILGTLVTFSAGWLLGPAWEKKR"
     gene            1069..1701
                     /locus_tag="P5079_11650"
     CDS             1069..1701
                     /locus_tag="P5079_11650"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRY30676.1"
                     /translation="MMSAFIDMVSVLQGHPWAPPAFVAVSALACLCLPITPFYVAGGF
                     LFGFWGGLLCNVAGTLLGTGLAFLLARRLAYGRFKAWILRKYPAVAALSESMDFRVVL
                     VARLTGFPPFWLMNYLPGLSGIRWRSYAAGTFAGTLPWIVIVSFFSHTLWNVLVASGL
                     KGLGHALAVHSRPLFYAGLILAGGMILTSFLKKRWAGKTPAGKHVSGKTV"
     gene            complement(1682..>2279)
                     /locus_tag="P5079_11655"
     CDS             complement(1682..>2279)
                     /locus_tag="P5079_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30677.1"
                     /translation="FWSRVHPRIYLGRGMALAALIFSAVVAYIAFSELAYAYMWRQTF
                     QLTETMSTYWDPVENWSVLYPKPWSHEAARLGETILHTFKPSKPTPTMYFSVTTRNGV
                     ETKEIAQVVDGFLLNLPKDRGIHLLESEKLVLPGGQPAYRIVYSEQTRRIPVKNETLF
                     VMDGTRLFYITVGAAPRWFDRQRVYLERLLYSLTLSSR"
BASE COUNT          340 a          719 c          731 g          489 t
ORIGIN      
        1 gccggccatc ctgctcttca ccgtggtggg gatcgtctac atctcgctgg gaggcatcaa
       61 ggccgtggtc tggacgaacc tcgtccaggc gctggtcatt ctgggcggcg gcatcgccac
      121 gctggtcttc ctcggatccc gcatcaccgg cgggggggcg accctctggc ggacggccgc
      181 ggccggcggc cggctgcacg tgctggatta cggcgttccc ttctcggctc ccgatttttg
      241 gacgtcgttc ttttccaacc cgaacatcct ctggctggcg gctttgaacg gcctgttcgg
      301 gtccatggcg gccttcggaa cggatcacga cctgatgcag cgtctgctga ccgtcgagac
      361 gcggcgggaa agtcagcgca ccatggcgtt gaccatcccc gtgagtctgg cggtgctgct
      421 catttatctt tccatcggca ccgggctcta tgtcttttac gcgcaaaacc cggccctgcc
      481 gttgccggac aacggggaca agatcttccc gcatttcatc gcccaggtga tgccgccgct
      541 tctccgtggg ctgctgctgt cggccatcgt gctggcgagc atcgactcgc cgctggcctc
      601 cctttcggct tctttcgtca cggatctgta ccggccgttc cgccgatggc tcggggaacg
      661 ggccggcccc gggggccggc tcggccggct ccaccggaga ctcccgcccg aagacgaagc
      721 ccacttttta cggatgtccc gctggggcgt ggcgggtttc ggcgcggtgt tggcggggct
      781 ggctttcttt ttcagtcact tcgaaaacat cctctggctg gcgttcaaga tcggcggggt
      841 gaccttcggg tcgctgctcg gcgtcttcct cctcggcctc ctcacggacc ggcgcggcga
      901 ccgggccaac gttctggcca tgacgttgag cgcgggagcc atgttcgtcc tgttggtcct
      961 ttccgaaaag cacatcctcc ctctcggctg gagctggctg gtgatcctcg gaacgctcgt
     1021 cactttttcg gcggggtggc tcctcggccc ggcatgggag aaaaaaagat gatgagcgcc
     1081 ttcatcgaca tggtctccgt tttgcaggga cacccctggg cgccgccggc gttcgtggcg
     1141 gtgagcgccc tggcctgtct ctgcctgccg atcacccctt tctacgtggc ggggggtttt
     1201 ctcttcggtt tttggggcgg cctcctctgc aacgtggccg ggaccctgct cggcaccggg
     1261 ctggcgttcc ttctggcccg ccgcctcgcc tacggacgat tcaaggcctg gatccttcga
     1321 aaatatccgg cggtggccgc tttgtccgag agcatggact tccgcgtggt cctcgtggcg
     1381 cggctgaccg gttttccgcc gttctggttg atgaactacc ttcccggact ctccggcatc
     1441 cggtggcggt cctacgcggc cggcacgttc gccgggacgt tgccctggat cgtcatcgtc
     1501 agtttttttt cgcacacgct ctggaacgtg ctcgtggcgt cgggcctgaa agggctgggc
     1561 cacgcgctgg cggtgcacag ccggccgctg ttctatgcgg gattgatcct ggcggggggg
     1621 atgattctga cgtcgttcct aaaaaagcgg tgggcgggaa aaacgccggc ggggaaacac
     1681 gtcagcggga agacagtgtg agggagtaca ggagcctttc cagataaacg cgttggcggt
     1741 cgaaccagcg gggggcggcc ccgacggtga tgtagaagag ccgcgtgccg tccatcacga
     1801 acagcgtttc gttcttgacg gggatgcggc gggtctgttc actgtaaacg atgcgatagg
     1861 ccggctgacc gcccggcaaa accagctttt cgctctccaa cagatggatc cccctgtcct
     1921 tcggcaggtt cagaaggaac ccatccacca cctgggcgat ctccttcgtc tccacgccgt
     1981 tccgcgtggt gacggaaaaa tacatcgtcg gcgtgggttt ggaaggcttg aacgtgtgca
     2041 ggatcgtttc acccagccgc gccgcctcat gggaccacgg tttcggataa aggacgctcc
     2101 aattctccac ggggtcccag taggtgctca tcgtctcggt gagctgaaag gtttgccgcc
     2161 acatataggc gtaggccagt tcgctgaaag cgatgtaggc gacgaccgcg ctgaagatca
     2221 acgccgccaa ggccatgccc ctccccaaat aaatgcgggg atgcacgcgg ctccaaaaa
//
LOCUS       DAOTFD010000318         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_42128_length_2197_cov_4.540616, whole genome shotgun sequence.
ACCESSION   DAOTFD010000318 DAOTFD010000000
VERSION     DAOTFD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42128_length_2197_cov_4.540616"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="P5079_11660"
     CDS             complement(<1..748)
                     /locus_tag="P5079_11660"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRY30678.1"
                     /translation="MTTSNDRFVPSPRPQVEHFEPYLPGRSMGQIKKEYGLKRVIKLA
                     SNENPLGPSPRAIAALRKHAPAMHLYPDGASTDLRRAVSEHLGVKFGQVILGAGSDEI
                     IELLGKTYLAPEDDIVVSAHAFIRYRMAGELMGARVRTVPMKNLTHDLDALAAAVSDK
                     TKFVFVANPNNPTGTYNTRADMERFLSSLPAHAVPVIDEAYFEYARMKKDYPDSLDFF
                     KAGRNVVVLRTFSKIHGLAGLRVGYGVAPEL"
     gene            complement(791..1876)
                     /gene="pheA"
                     /locus_tag="P5079_11665"
     CDS             complement(791..1876)
                     /gene="pheA"
                     /locus_tag="P5079_11665"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRY30679.1"
                     /translation="MKEKESDKELDALRKKIDGLDDQLLRLLNERARHAQKVGEIKIQ
                     RKEEIYASNRERQILDRLVAESQGPLPEESVEEIFRAIINNCRLLQKRLRIAYFGPEA
                     TFTHLAATKHFGQAAEYVPFKSIADVFDEVERDRADYGVVPIENSTEGVVNHTHDMFM
                     ESNLVICAEREDKISQCLLGVQDSLKKVKLVYSHTQPLAQCRHWLDAHLPGVALHEAA
                     STADAAAHAALDPAAAAVASPLAAEIYSLNILASNIQDSPHNATRFLIIGKKMAGPSG
                     RDKTSVLLSIKDRVGALHDLLMVFKEEKLNLTKIESRPTKKRAWEYVFFIDVEGHVAD
                     ANMKRVLDKLQPHCLFVKILGSYPRGD"
BASE COUNT          336 a          645 c          763 g          453 t
ORIGIN      
        1 ggagttccgg ggccacgccg tatcccacgc gcagcccggc caggccgtgg atcttcgaaa
       61 aggtgcgcaa caccacgacg ttcctccccg ctttgaagaa atccagggag tccgggtagt
      121 cctttttcat gcgggcgtat tcgaaatacg cctcgtcgat gacgggcacg gcgtgcgccg
      181 gcagggagga gaggaaccgt tccatgtccg cccgggtgtt gtaggtgccg gtggggttgt
      241 tggggttggc gacgaagacg aacttggtct tgtctgaaac cgcggcggcg agggcgtcca
      301 ggtcgtgcgt caggtttttc atcggcaccg tgcgcacgcg ggcgcccatc agctcccccg
      361 ccatccggta gcggatgaag gcgtgggcgg agacgacgat gtcgtcttcc ggggcgaggt
      421 aggtcttgcc caggagttcg atgatctcgt cggagccggc gcccaggatc acctggccga
      481 acttcacgcc aaggtgttcc gacaccgcgc ggcgcaggtc ggtgctggcc ccgtcgggat
      541 agaggtgcat ggcgggcgcg tgtttgcgca gcgcggcgat ggcgcggggc gacggcccga
      601 gagggttctc gttggaggcg agcttgatga cccgcttgag gccgtactcc tttttgatct
      661 gtcccatgga gcgtccgggc aggtagggct cgaaatgttc cacctgcggc cggggactcg
      721 gaacgaatcg gtcgttggat gtcgtcatgg ggtcgtgtct ccctctgaaa ggcggtggcg
      781 cggcggggtt tcagtcgccg cggggatagc tgcccagtat cttcacaaaa aggcagtgcg
      841 gctggagctt gtccaggacg cgtttcatgt tggcgtccgc cacatggcct tccacgtcga
      901 tgaagaaaac gtattcccag gcccgtttct tcgtcgggcg ggactcgatc ttcgtcaggt
      961 tcagtttctc ctccttgaac accatcagca ggtcgtgcag ggcccccacg cggtccttga
     1021 tggagagcag gacggaggtt ttgtcgcgtc ccgacggccc cgccatcttc ttgccgatga
     1081 tgaggaagcg cgtggcgttg tgcggggagt cctggatgtt gctggcgagg atgttcagcg
     1141 aatagatctc ggccgccagg gggctggcga cggccgccgc cgccgggtcg agcgcggcgt
     1201 gcgccgcggc gtccgccgtg gaggccgcct cgtgcagggc cacgccgggc aggtgggcgt
     1261 cgagccagtg ccggcactgg gccagcggtt gcgtgtgcga atagaccagc ttgacctttt
     1321 tcaacgagtc ctgaacgccc aggaggcact gcgatatctt gtcctcccgc tcggcgcaga
     1381 tgaccaggtt ggattccatg aacatgtcgt gggtgtggtt caccacgccc tcggtggagt
     1441 tctcgatggg caccacgccg tagtcggcgc ggtcccgttc cacctcgtcg aacacgtcgg
     1501 cgatggactt gaaggggacg tactcggcgg cctgcccgaa gtgttttgtg gccgccaggt
     1561 gcgtgaaggt ggcctccggc ccgaaatagg cgatgcggag ccgtttctgc aggagacggc
     1621 agttgttgat gatggcgcgg aagatctcct cgacggattc ctccgggagg gggccctggc
     1681 tctcggccac gagccggtcg aggatctgcc gttcccggtt ggaggcgtag atctcctcct
     1741 tgcgttggat tttgatctcc cccacttttt gggcgtgccg ggcgcgctcg ttcagcaggc
     1801 gcaagagctg gtcgtccagc ccgtcgattt ttttccggag ggcgtcgagt tccttgtccg
     1861 attctttttc tttcatggtt cggtttttat ggccagtacg gcggccatcc ttttctcggt
     1921 tttattccgt cggtacgaaa aaaatcggtc atcgtgccgc gaacagtgcg gggcggcgga
     1981 aaaacggttt gccgggaccc ccgcggcggc cgcctcggcg cggaggcacg cggtcaaatc
     2041 aaggaataaa cgcgtcccgc ggcggatcac cccgcgggga cggtctttga atttccgtgc
     2101 caccgcgggc cccacctcgc agcagcacgg ctggatgtgc gggccgacca gcaccgtcat
     2161 cttcgccggt tttgcgccga aacgtttccg taaaaga
//
LOCUS       DAOTFD010000319         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_43259_length_2162_cov_4.513052, whole genome shotgun sequence.
ACCESSION   DAOTFD010000319 DAOTFD010000000
VERSION     DAOTFD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43259_length_2162_cov_4.513052"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="P5079_11670"
     CDS             <1..369
                     /locus_tag="P5079_11670"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRY30680.1"
                     /translation="LANEPRRPGRTPLPPNIRKDKIAVTAMVRISCSARHGGKKEPCA
                     SCRELLEYALQRLDLCPFGENKTACSQCPIHCYKPDMRSRIQDVMRFAGPRMVFYHPW
                     MALRHWARRGAPPPPRPGRV"
     gene            567..1535
                     /locus_tag="P5079_11675"
     CDS             567..1535
                     /locus_tag="P5079_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HRY30681.1"
                     /translation="MYRKEIKVLDCTIRDGGLINDYQFSEKFVKAVYRAICDSGVDYL
                     ELGKKLVESEDYPRSKYGPWNFSDDDVVQRIVDSYQGKHRPQLSVMFDVGRVDIATLR
                     DKKDSPIDMVRTACYVHQVDKGLDLVKRCKEKGYATTLNIMAVSAAIEAELMEALEDV
                     AKTPELDFLYLVDSYGAYYSEQVTHMLNLYGKYVPREKLAFHGHNNIQLAYSNTQQAI
                     IDGVNMLDATINGIGRGAGNCCLELLLGFLKNPKFDVRPIYRVIQEEFVPLRQEIEWG
                     YNDIYGISGLMNQHPREAMKFRANREKRDNCYDFHELVTRQDVGPV"
     gene            1537..>2162
                     /locus_tag="P5079_11680"
     CDS             1537..>2162
                     /locus_tag="P5079_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY30682.1"
                     /translation="MPPFKLARPLVFFDLETTGLMQEIDRVIELGLLKLFPDGRRQKL
                     LQRFNPGIKIPAESSAVHGITNKDLEGQPKFFEKAKELFDLFADSDLGGYNIKRFDIP
                     MLTKEFEIAGLNFSMEGRRIVDPSVIFRVREPRSLSAAYKFYVGKELTDAHSAEADND
                     ATFEVFLAQMERYPDLPRDVEGLHALGNAPDPGWVDADGKLVWRDGEA"
BASE COUNT          446 a          677 c          666 g          373 t
ORIGIN      
        1 ctggccaacg agccgcgccg gccggggcgg acgcccctcc cgccgaacat ccggaaagac
       61 aaaatcgccg tgacggcgat ggtgcggata tcttgttccg cccgtcacgg cgggaagaag
      121 gaaccgtgcg cttcctgccg tgaactgctg gagtacgccc ttcaacggct cgatctctgc
      181 cctttcgggg agaacaagac cgcctgctcc caatgtccca tccattgtta taagcccgac
      241 atgcggtcgc ggatacagga cgtgatgcgt tttgccgggc cgcgcatggt gttttatcac
      301 ccctggatgg ccctgcgcca ttgggcccgg agaggcgcgc cgccgccccc aagacccggc
      361 cgcgtttaag gggcgccgtt ccccatcccc gtcccgcgtc aaaaattggg cttggatggg
      421 cccgccaaag tttgtaaatt aatgacacag cccccgcgca cgatgcgaaa acgcctccct
      481 cttcggggga ggggaacccc ggggctggga taagcaggga atcccgccgt acgtgtcctg
      541 cccaacaatc atcaatcgga ggagcgatgt acaggaaaga gatcaaggta ttggattgca
      601 ccatacggga cggggggttg atcaacgact accagttttc cgagaagttc gtgaaggccg
      661 tctaccgcgc catctgcgat tccggcgtgg attacctgga gttgggcaag aaactggtgg
      721 agagcgagga ttacccgcgc tccaaatacg gcccctggaa tttttccgac gacgacgtgg
      781 tgcagcgcat cgtggacagc tatcagggaa agcaccggcc gcagttgtcg gtgatgttcg
      841 acgtgggacg ggtggacatc gccaccctgc gcgacaagaa ggacagcccc atcgacatgg
      901 tgcgcacggc ctgctacgtg caccaggtgg acaaggggct cgacctggtg aaacgctgca
      961 aggagaaggg ctacgccacc acgctcaaca tcatggcggt gtcggcggcc atcgaggcgg
     1021 aactgatgga ggccttggaa gacgtggcca agacgccgga gttggatttc ctctatctgg
     1081 tggacagcta cggcgcctac tattccgaac aggtgaccca catgctgaac ctgtacggca
     1141 agtacgtgcc ccgcgaaaag ctggcttttc acggacacaa caacatccag ctggcctact
     1201 ccaacacgca gcaggcgatc atcgacgggg tcaacatgct ggacgccacc atcaacggca
     1261 tcggccgcgg cgccggcaac tgctgtctgg aactcctgct gggtttcctg aaaaacccca
     1321 agttcgacgt gcggcccatc taccgggtga tccaggagga gttcgtcccg ctgcgccagg
     1381 agatcgagtg gggctacaac gacatctacg gcatctccgg cctgatgaac cagcacccgc
     1441 gcgaggccat gaagttccgc gccaaccggg agaagcggga caactgctac gacttccacg
     1501 aactggtgac ccgccaggac gtggggccgg tctgagatgc cgccgttcaa gctcgcccgg
     1561 ccgctcgtct tcttcgacct ggagaccacc ggtctcatgc aggagatcga ccgcgtcatc
     1621 gagctcggcc ttctcaagct ctttcccgac ggccgccggc agaagctgct ccagcgtttc
     1681 aatcccggga taaagatccc ggcggagtcc tccgcggtgc acggtatcac caacaaggac
     1741 ctggaggggc agccgaagtt tttcgagaag gcgaaggaac tcttcgacct cttcgccgat
     1801 tcggatctgg gcggatacaa catcaagcgg ttcgacatcc ccatgctgac caaggagttc
     1861 gagatcgccg gcctcaactt ctccatggaa gggcggcgga tcgtggatcc cagcgtcatc
     1921 ttccgcgtgc gggaaccgcg gtccctttcg gccgcctata aattttatgt ggggaaggaa
     1981 ttgaccgacg cccacagcgc cgaggcggac aacgacgcca ccttcgaggt gttcctggcc
     2041 caaatggaac gctaccccga ccttccccgg gacgtggaag ggctgcacgc cctggggaac
     2101 gcccccgacc ccggctgggt ggacgccgac ggcaagctgg tctggcgcga cggggaggcc
     2161 tt
//
LOCUS       DAOTFD010000320         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_43481_length_2156_cov_4.279391, whole genome shotgun sequence.
ACCESSION   DAOTFD010000320 DAOTFD010000000
VERSION     DAOTFD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43481_length_2156_cov_4.279391"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /gene="tal"
                     /locus_tag="P5079_11685"
     CDS             complement(<1..1425)
                     /gene="tal"
                     /locus_tag="P5079_11685"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRY30683.1"
                     /translation="MNPLVELGKFQQSVWYDNIRRKLLLSGELKKLIVEDGVRGVTSN
                     PTIFEKALSAGDDYDADIRALAARGLPVKDIFDALSVKDIQLAADLFAPLYKSSRGTD
                     GFVSMELAPSLAHDVKGSVREARRLWSLVRRPNLMIKIPATKEGVKATEQCLLEGINV
                     NVTLIFSVERYGQVIDAYRAALERRARAKKDLSRVASVASFFVSRIDTAVDKLLQERL
                     SVVQDAKQRETIQGLAGKSAVANAKVAYQLYKERFGGEWFAALSKKGARVQRPLWAST
                     GTKNPAYSDVLYVEELIGENTVNTMPPATIDAFREHGKPRATLEENVASAQETLRRLG
                     DVGIDFGRVTDQLEAEGEKLFSDSYDKLIRGLEEKRGRILLSLRTGASFSLGHYEADV
                     NAALQNMQKGKVADRLWARDASLWKSEPALQSAIRNRLGWLLLSSSMREKAARLGEFV
                     EKIRKAGFKRVVLLTVDQRTRCLVP"
     gene            complement(1528..>2156)
                     /locus_tag="P5079_11690"
     CDS             complement(1528..>2156)
                     /locus_tag="P5079_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY30684.1"
                     /translation="IFTHDSAGVGEDGPTHQPVEHLAALRALPKMRVFRPADANETVV
                     AWRLALERRDGPTALVLTRQKLPVLDRTKFASAENVSKGGYVLSPSSDGKPRVVLIAT
                     GSEVHVALRAQEELARRNISAQVVSLPSWELFDAQPPDYRDSVLPPGVAARVAVEAGA
                     SLGWHKYVGPRGALVTLDRFGASAPGEIALEKLGFNAGNVVDKALALL"
BASE COUNT          383 a          741 c          631 g          401 t
ORIGIN      
        1 cggcaccagg cagcgcgtcc gctggtcgac cgtcaacagg accacccgct tgaaaccggc
       61 tttccgtatt ttttcgacga actcgccgag acgggccgcc ttctcccgca tgctcgaaga
      121 caacaacagc cagcccaagc ggttgcgaat ggccgactgc aaggccggtt cggacttcca
      181 cagggaggcg tcgcgggccc agagacggtc cgccaccttc cccttctgca tgttctgcag
      241 agcggcgttc acgtcggcct cgtaatgacc gagcgaaaaa gaagcccccg tgcgcagaga
      301 aaggaggatg cgccccctct tctcctccaa gccccggatc aacttatcgt aggaatccga
      361 gaagagtttc tccccttccg cctccagttg gtccgtcacc cggccgaagt cgatgcccac
      421 gtcgcccagt cggcgcagcg tctcctgcgc cgacgccacg ttttcctcca gagtggcgcg
      481 cggttttccg tgctcccgga aggcgtcgat ggtggcgggc ggcatggtgt tgaccgtgtt
      541 ctcgccgatc agttcctcca cgtacaggac gtcggaatac gcggggtttt tcgtcccggt
      601 gctggcccag aggggccgct gaacgcgcgc gcctttcttc gacagcgccg caaaccattc
      661 gccgccgaac cgctccttgt agagctgata cgccaccttg gcgttggcca cggcgctctt
      721 gccggccaga ccctggatcg tctcccgttg tttcgcgtcc tgcaccaccg agagccgctc
      781 ctgcaaaagt ttgtccacgg cggtatcgat gcggctcacg aagaaactcg ccaccgaagc
      841 cacccgcgac aggtctttct tggcccgggc gcgccgttcc agggccgccc ggtaggcgtc
      901 gatgacctgc ccgtagcgct ccacggaaaa aatcaaggtc acgttgacgt tgatgccttc
      961 aaggagacat tgttccgtcg ccttcacccc ctccttcgtg gcggggatct tgatcatcag
     1021 attgggccga cgcaccaagc tccacagccg gcgggcctcc cggacgctgc ccttcacgtc
     1081 gtgcgccagc gagggggcca gctccatgga cacgaacccg tccgtccccc gggaactttt
     1141 gtagagcggg gcgaagaggt ccgcggccag ctggatgtcc ttgacggaca acgcgtcgaa
     1201 gatgtccttg accggcagtc ccctggcggc cagggcgcgg atatcggcgt cgtaatcgtc
     1261 gccggcggaa agggccttct cgaagatggt gggattcgag gtgacgccgc gcaccccgtc
     1321 ctcgacgatc agcttcttca actcgccgga caaaaggagt tttcgccgga tattgtcgta
     1381 ccacacgctc tgttggaatt ttccaagttc caccaaggga ttcatgttct ggttcctccg
     1441 ttgttattca aaaaattagt ccccccttat ggggagaggt ccgcaactct tccgccgcat
     1501 caaaaaatat tcgtctatcc aaaccattca cagcagcgcc agggccttgt ccaccacgtt
     1561 cccggcgttg aaccccagtt tttccagggc gatctctccc ggggcggagg cgccgaaacg
     1621 atcaagggtc accaacgcgc cgcggggacc cacgtatttg tgccaaccca gcgaagcgcc
     1681 cgcctccacg gccacgcgtg ccgcgacccc ggggggcaac accgagtccc gataatccgg
     1741 cggctgcgcg tcaaaaagtt cccagctcgg caggctcacc acctgcgccg agatgttccg
     1801 gcgggcgagt tcttcctggg ccctcagcgc cacatgaact tccgaaccgg tggcgatcag
     1861 cacgacccgc ggtttgccgt ccgacgaggg agacaggacg tagccgcctt tcgatacgtt
     1921 ctccgccgaa gcgaatttcg tccggtccag caccgggagc ttctgccggg tcagcaccag
     1981 agccgtcggg ccgtcgcggc gttccaacgc cagccgccac gcgacgacgg tctcgttggc
     2041 atcggcgggg cggaacaccc tcatcttcgg cagggcgcgc aacgcggcca aatgttccac
     2101 cggttgatgg gtgggcccgt cctcccccac gcccgcgctg tcgtgcgtga aaatat
//
LOCUS       DAOTFD010000321         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_43864_length_2144_cov_4.129727, whole genome shotgun sequence.
ACCESSION   DAOTFD010000321 DAOTFD010000000
VERSION     DAOTFD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43864_length_2144_cov_4.129727"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="P5079_11695"
     CDS             complement(<1..747)
                     /locus_tag="P5079_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30685.1"
                     /translation="MTPGMRGMKRYLGLGLAALIALGSGGPAWSGNVELCRHHQFDSG
                     ARLKNAIAVAVDETDRRLYVSGYNSHNILLYDLDARGVVHQTTGIHYPHIVRFHPVSQ
                     KVVALGFDENWNGLLVVYDKHLRELHRVVLGYYPYSALLSEDGRTAYVGVYNQIVAVN
                     LEDGVSSTFVSLGSVPYSYPFAMAMDPSRGRLLAVGTGWVNNGQEWVLRSAVIPIGMA
                     DRQLGPELVLGDRLYSYDAALDRDELFIANI"
     gene            complement(744..1160)
                     /locus_tag="P5079_11700"
     CDS             complement(744..1160)
                     /locus_tag="P5079_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30686.1"
                     /translation="MLKKNVGMMLAVILAAGVLAADETIVRPTREEVRSQLRRAPRFA
                     GPSAAAPDAFQVKYSLAEAAPLSVTLLDLSRIPLRTFHVAAGEKGARAGENVLTVWDG
                     RDSAGRDAPSGEYWVCLSFRHADGRVENKRFRINKP"
     regulatory      complement(1244..1356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1491..>2144)
                     /locus_tag="P5079_11705"
     CDS             complement(1491..>2144)
                     /locus_tag="P5079_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRY30687.1"
                     /translation="LFDADIYGPNIPLMLGASGPPAARDNKLIPLEAHGVKLMSMGFL
                     ADENTPMIWRGPMVHGAIRQLLRETDWGELDYLVVDLPPGTGDAPLTLIQSVPLSGVV
                     LVTTPQDVALLDGVKGIRMFRKLNVPILGLVENMSGFVCPNCHHETAIFTKGGGEKEA
                     ARQKLTFLGAIPLDPAIAQGGDKGVPLVVGQPNSPQTKALRWLAQIVAGQISIANMK"
BASE COUNT          399 a          630 c          713 g          402 t
ORIGIN      
        1 gatgttggcg atgaacagtt cgtcgcggtc cagcgcggcg tcgtaggaat agagacggtc
       61 tcccagaacc agttccggcc ccaactgccg gtcggccatc ccgatgggga tcaccgcgct
      121 gcgcagcacc cattcctggc cgttgttcac ccagcccgtg cccaccgcca ggagacggcc
      181 gcgcgaaggg tccatcgcca tggcgaaggg ataggagtac ggcacgctgc cgagggagac
      241 gaaggtcgag gagacgccgt cctccaggtt gacggccacg atctggttat agacgccgac
      301 gtaggcggtg cggccgtctt ccgacaagag ggcgctgtac gggtaatagc ccagcaccac
      361 gcggtgcagt tcccggagat gtttgtcgta gaccaccagg aggccgttcc agttttcgtc
      421 gaacccgagc gcgacgacct tttggctgac ggggtggaac cggacgatgt gcgggtagtg
      481 gatgccggtg gtttgatgga cgacgccgcg ggcgtcgagg tcgtaaagga ggatgttgtg
      541 gctgttgtaa ccgctgacgt agagccggcg gtcggtctcg tcgacggcca cggcgatggc
      601 gttcttgaga cgggcgccgg aatcgaactg atgatgacgg caaagttcca cgttgcccga
      661 ccacgccgga ccgccggaac ccagggcgat cagcgccgcc aggccgaggc cgagataccg
      721 tttcatgccg cgcattcccg gggtcatggt ttgttgatcc tgaaccgttt gttttccacc
      781 cgcccgtcgg cgtggcggaa agagaggcag acccaatatt ctcccgaagg cgcgtcgcgg
      841 ccggcggagt cacggccgtc ccacaccgtc aacacgttct ccccggcccg cgcccctttt
      901 tcgccggcgg ccacatggaa cgtgcggaga gggatccggc tcaaatccaa cagggtgacg
      961 gagagcgggg cggcttcggc gagggagtat ttgacctgaa aagcgtccgg cgcggcggcg
     1021 gaggggccgg cgaaacgagg ggcccggcgc aattgcgaac ggacctcttc ccgcgtgggg
     1081 cggacgatcg tttcatcggc ggccaacacc cccgccgcca agatcaccgc cagcatcatc
     1141 ccgacgttct ttttcaacat ccgtccgtcc tttcaataaa taaggctacc caccgtttgg
     1201 tagggtagcc tcgggaagac gtcttaaaac gtctttcttt ttaaacggcg gcccggccga
     1261 catggataga gggaacctca cggattcccc ctcggcgcta tctttagtcc cgaggctttg
     1321 cggccccgct tttcagcggg tgtgccttta tcgtcacttt gtatttatcc ccggaacgtg
     1381 ccgttgtcaa gggttgcgta aataaaattg acacggactt ttatccgatc cggggagagg
     1441 gtttgtgacg atgggaacgg ttgggaggaa accggtctcg aaaaccggcg ttatttcatg
     1501 ttggcgatgc tgatctgccc ggccacgatc tgggccagcc agcgcaacgc cttggtctgc
     1561 ggactgttcg gttgaccgac gacgaggggg acgcccttgt cgccgccttg ggcgatggcg
     1621 gggtcgagcg ggatggctcc caaaaaagtg agtttctgcc gggccgcctc cttttcgccg
     1681 ccgcccttgg tgaaaatggc cgtttcgtga tgacagttcg gacagacgaa cccggacatg
     1741 ttctccacca gtcccaagat gggcacgttc agtttacgga acatccggat gcccttcacg
     1801 ccgtcgagca gggccacgtc ctggggggtg gtgaccagaa ccacgccgga cagcggcacg
     1861 gattggatga gagtcaaggg ggcgtcgccg gtgccgggcg gcagatccac caccaagtaa
     1921 tcgagttccc cccaatcggt ttcgcgaagg agttgtcgga tggcgccgtg caccatgggg
     1981 ccgcgccaga tcataggggt attctcgtcg gccaaaaaac ccatggacat aagtttcacg
     2041 ccgtgggctt cgagcgggat gagcttgttg tcccgggccg cgggagggcc ggaggcgccc
     2101 agcatgagcg ggatgttggg accgtagatg tcggcgtcga aaag
//
LOCUS       DAOTFD010000322         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_44567_length_2123_cov_3.333656, whole genome shotgun sequence.
ACCESSION   DAOTFD010000322 DAOTFD010000000
VERSION     DAOTFD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44567_length_2123_cov_3.333656"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="P5079_11710"
     CDS             <1..1613
                     /locus_tag="P5079_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRY30688.1"
                     /translation="WGRLYDIGVVTGCLLYFSYWILDGLWTQGVYCDIGFLGRYALGV
                     THGLPVDSGDAFLRFFGRSFSLTLRPQHGPNEIYLTLPWIYLLGNTAAALHLSPFFWS
                     ALSLPLFYGIARRLYQSRVSATAGLILFAASTSHIAAVRLGLYSGSILLFWALAALGA
                     FLKWRTTRRTPWRMAGFFFLGIGSGCRCFFWWYVAAIAVVFGTVERKSWRARVLATGA
                     FWGSLGVFLVGVLPIVVANLTGKFYMFRFFLQNMLYTSETRPVMNLAYATNLGKRLIH
                     LKSLLNGTFFSRPDAPDLALPILFGTALIGVGLYAALSKDAHKKRFVLPAALIVLVFL
                     QTPFSPTSLDPHHLLILFPFICLTAASVFAFSLPKTLKFPLWLVGGFLVAWSGYNGYV
                     RVHHREAHRQLHGGSDVKWNTLGRVVAWCRDKDLRTLGLADTGLMDSFIYLSGGDIRI
                     KEVFAAAYRSPDLDGQREEFRNYLRRDASGYYLFRGKGQNPWVPFFNDFLRIVREEGR
                     DARLAMTFVDPDGIEAYAVYHVASKKTP"
     gene            1744..>2123
                     /locus_tag="P5079_11715"
     CDS             1744..>2123
                     /locus_tag="P5079_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30689.1"
                     /translation="MEFGIHLIKDPIFVVALLVFLTGAGVLVWAIGQLRMAGVRPRQS
                     GGGRDAANPAMVVSGMGGPLPEPSLVSSASKPAAGTHPMENDAAVMYSVLEERFAELS
                     KRIAQVETTPAASRATDKSATALPA"
BASE COUNT          338 a          713 c          607 g          465 t
ORIGIN      
        1 cctggggccg tctgtacgac atcggggtcg tgacgggatg tcttctgtat ttttcctact
       61 ggatcctcga cggtctctgg acgcagggcg tctattgcga catcggcttc ctcgggcgtt
      121 acgccctggg ggtcacccac ggactgccgg tggacagcgg ggatgcgttc ctccgcttct
      181 tcggccgctc gttctccctg acgttgcgtc ctcaacacgg cccgaacgaa atctatctca
      241 ccctcccgtg gatctacctg ctggggaaca ccgccgccgc gctgcatctc tctccctttt
      301 tctggtccgc cctgagcctc cctttgtttt acggcatcgc ccgccgactt taccaaagcc
      361 gggtgagcgc caccgccggt ctgatcctgt tcgcggcctc caccagccac atcgcggccg
      421 tccggctggg cctgtattcc ggatccattc tccttttctg ggccctggcc gcgctgggcg
      481 ccttcctgaa atggcggacc acccgtcgca cgccctggcg gatggccgga tttttctttt
      541 tgggtatcgg gtcgggatgc cgctgcttct tctggtggta tgtggcggcg atcgccgtcg
      601 tcttcggaac ggtggaaagg aaatcctggc gggcgcgcgt gttggcgacc ggcgccttct
      661 ggggttcgct gggcgtcttc ctcgtcggcg tcctgcccat cgtcgtcgcc aacctgaccg
      721 ggaaattcta catgttccgg tttttccttc agaacatgct ttacacctcc gagacgcggc
      781 ccgtgatgaa cctcgcctac gccacgaacc tgggcaaacg cctgatccat ctgaaatccc
      841 tgctcaacgg cacctttttt tcccggccgg acgccccgga ccttgccctc cccatcctgt
      901 tcgggaccgc gctgatcggc gtcggcctct acgcggccct ttccaaagac gcgcacaaaa
      961 aacgtttcgt cctccccgcg gccctcatcg tcctcgtctt cctccagacg cccttttcgc
     1021 cgacgagcct ggacccgcat cacctgctga tcctgttccc gttcatctgc ctcacggcgg
     1081 cctcggtgtt cgccttttcg cttccgaaaa cgctgaaatt cccgctgtgg ctggtgggag
     1141 gattcctcgt cgcgtggtcc ggatacaacg gttatgtccg cgtccaccac cgggaagccc
     1201 atcggcagct gcacggcggc agcgacgtca aatggaacac cctgggacgg gtggtggcgt
     1261 ggtgccggga caaggacctc cggaccctgg ggctggccga cacagggctg atggattcgt
     1321 tcatctatct cagcgggggg gacatccgca taaaagaagt cttcgcggcg gcctatcgct
     1381 ctccggacct cgacggccaa cgggaagaat tccggaatta cctccgccgg gacgcgagcg
     1441 gctattatct tttccgtggg aaaggccaaa acccgtgggt cccgttcttc aacgattttc
     1501 ttcgcatcgt tcgggaagaa gggcgggacg cccggttggc catgaccttt gtggatccgg
     1561 acgggatcga ggcgtacgcc gtttatcacg tcgcttcaaa gaaaacgccg tgaccccctc
     1621 cctcctcata cagattgtta caatagcccg ctcccccgtt catcgaaaaa aggcttccct
     1681 ctctcgtccc gcctccactc aaatcccatt gacaattcaa cctatttccg ttaaagtttt
     1741 agcgtggaat tcggcataca tttgatcaaa gacccgatat ttgtggtggc gctccttgtt
     1801 ttcctgaccg gagcgggtgt cttggtctgg gccatcggcc aactgcgcat ggcgggcgtg
     1861 cggccacgcc aaagcggcgg aggccgcgac gcggccaacc cggcgatggt ggtttccggg
     1921 atgggggggc cgctgcccga accgagcctt gtgtcgtcgg cctccaaacc ggcggccggg
     1981 acccatccga tggaaaacga cgccgcggtg atgtacagcg tcctggaaga acggttcgcg
     2041 gaactctcca aacgcatcgc ccaggtcgaa acgacgccgg ccgcctctcg ggccacggat
     2101 aaatccgcaa ccgccctgcc ggc
//
LOCUS       DAOTFD010000323         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_44832_length_2116_cov_3.494420, whole genome shotgun sequence.
ACCESSION   DAOTFD010000323 DAOTFD010000000
VERSION     DAOTFD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44832_length_2116_cov_3.494420"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..338
                     /locus_tag="P5079_11720"
     CDS             129..338
                     /locus_tag="P5079_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30690.1"
                     /translation="MAAFFAAFWTEWRSRAWAYGAVVLVSILLSFVACLLLDQRDALR
                     RHVASETSDIAWIVFLANHADRPSI"
     gene            555..884
                     /locus_tag="P5079_11725"
     CDS             555..884
                     /locus_tag="P5079_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30691.1"
                     /translation="MEEVAYDTARVRRLDALQRLLYYLDISLLSLLWGGAVLLVLLTG
                     RLLFFPKMTFQGKVLLFCALAGFAGSLLGLWAAGTFVAHIFWRVLWAGTAAGYLVALV
                     QNLFQES"
     gene            887..2089
                     /locus_tag="P5079_11730"
     CDS             887..2089
                     /locus_tag="P5079_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY30692.1"
                     /translation="MLVGRPRRAAPPRRVFSGRALVWIVFLLCMFPAVTFAVKIDGQI
                     RSKQKDLRKIQGELEKKADERSVAARKEKELAAEVEKISRELKASRAALKESQSQVRQ
                     TENKRKSVEERLWASRLEIGQWADILSRELRTFYMRRLALGAAAQVETAYRRAALKEK
                     TQSLSFAQQHHAQVEGLREELVGLEIELQKLRLNREREEKRAETAGKQMQSLYKTVAG
                     RRAILEQEIRELQSSAEEMEKLLQTLLRRQQEELAKAAAQKPPAKKGAFAAVARKPGK
                     LPWPVDGTVIERYGRSKHPELDTYVFSNGIKLRPTASAAIRTVEKGEVLYAGEFMSYG
                     LMALVQHPDRLFSIYGHLGQLNVARGQKVSVGESIGVPGQDDQGRPVVYFELRVGGVA
                     VDPLLWLK"
BASE COUNT          408 a          644 c          656 g          408 t
ORIGIN      
        1 ccaccggctt attttgctaa aataaaaaag ttgtgccgcc ccgccgcagg ggaaaacctc
       61 aaggaggaga caacggagag cccggccttc catcggcccg gggacggatt tttctctctc
      121 aaaaaaccgt ggccgcattt ttcgcagctt tctggacgga atggagatcc cgggcgtggg
      181 cctacggcgc cgttgtcttg gtcagcatcc tcctttcctt cgtcgcctgc cttctgttgg
      241 accagcggga cgcccttcgg cggcatgtcg cctcggagac ttccgacatc gcatggatcg
      301 tcttcctcgc caaccatgcc gaccggccct ccatctaaga gaccatccag tctttcccgg
      361 gcatcaaggc catccggttc atctccaaag aagaagccct cgacaacgta aaaaaggaac
      421 cgaccttctc ccaaaccctg acgctggccg ggcgcaaccc gttcccggat tcgttcgatg
      481 tccgttgggc cccgtttttc ctccggagcg attatctcga acattccgcc cataagatca
      541 aagagctgga cggggtggaa gaagtggcct acgacaccgc ccgggtgcgg cggctggacg
      601 cgcttcagcg cctcctttat tatctggaca tttccctgct gagcctgttg tggggcgggg
      661 cggtgcttct ggtgcttttg acgggccggt tgttgttctt tccgaagatg acgtttcagg
      721 ggaaagtcct tctgttctgc gctctcgcgg ggttcgccgg ttcgctcctg ggtttatggg
      781 cggccggcac cttcgtggcc catatattct ggcgggtgct ctgggcggga acggcggcgg
      841 ggtatctcgt ggcgctggtc cagaacctct ttcaagaatc ctgatcatgc tcgtgggacg
      901 tccccggaga gcggccccgc ctcggcgcgt tttttccggc cgcgcccttg tctggatcgt
      961 tttccttctt tgcatgtttc cggcggtgac cttcgccgtg aagatcgacg gccagatccg
     1021 ctcgaaacag aaggacctgc ggaagataca gggcgaactg gagaaaaagg ccgacgaacg
     1081 gtcggtcgcg gcgcgcaagg agaaggaact ggccgcggaa gtggaaaaga tttcgcggga
     1141 actgaaggcc tcccgcgccg cgctcaagga gtcccagtcc caggtccgcc aaaccgaaaa
     1201 caagcggaaa tccgtggagg agcgcctgtg ggcgtcccgg ttggagatcg gccagtgggc
     1261 cgacatcctc tcgcgggaac tgcggacatt ttacatgcgc cggttggcgc tgggcgccgc
     1321 ggcccaggtg gagaccgcct accgccgggc cgccctgaag gagaagacgc agagtctctc
     1381 cttcgcgcaa cagcaccacg cccaggtgga ggggctgcgc gaggaactcg tcggcctgga
     1441 gatagaactc cagaaactcc gtttgaaccg agaacgggag gaaaaacggg ctgaaactgc
     1501 cggcaaacag atgcagtcgc tttacaagac cgtcgccggc cggcgggcca ttctggaaca
     1561 ggagatccgc gaacttcagt cttcggcgga agaaatggaa aaactcctgc agacgctcct
     1621 ccgccgccaa caggaagagc tggccaaggc ggcggcccag aaacctccgg ccaaaaaggg
     1681 cgctttcgcc gccgtggccc ggaaacccgg caagcttccc tggcccgtgg acggcaccgt
     1741 tatcgaacga tacggccggt cgaaacatcc ggagttggac acgtacgttt tttccaacgg
     1801 catcaagctc cggcccaccg cctcggcggc catccgcacc gtggaaaaag gcgaggtgct
     1861 ctacgccggc gaattcatga gttacgggct gatggccctg gttcagcatc cggaccggct
     1921 tttcagcatc tacggtcacc tggggcaact caacgtggcc cgcggacaga aggtgtccgt
     1981 cggggaatcc atcggcgtcc ccggacagga cgaccaaggg cgcccggtgg tctatttcga
     2041 gttgcgcgtg ggcggcgtgg cggtggatcc gctgttgtgg ctcaaatgac gattttttca
     2101 acgccaggaa gtttca
//
LOCUS       DAOTFD010000324         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_45193_length_2106_cov_3.896636, whole genome shotgun sequence.
ACCESSION   DAOTFD010000324 DAOTFD010000000
VERSION     DAOTFD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45193_length_2106_cov_3.896636"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="P5079_11735"
     CDS             <1..585
                     /locus_tag="P5079_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY30693.1"
                     /translation="LLKPIPRWSGVSLPITSFGQEVSVTALQLACAYSAIANGGVLLE
                     PRLFVEAVDQIGGRKTWETPTAIRRVISEKTAARLRDILQGVVLRGTGKDALLDGWTV
                     AGKTGTAQKIDPRTRKYSADKFVASFCGFVPAVKPRLTIVVVYDEPQGVSWGGYNAGP
                     VFRNVAWHAMTYLGVSSDKETRLARRKLREVSPL"
     gene            582..>2106
                     /locus_tag="P5079_11740"
     CDS             582..>2106
                     /locus_tag="P5079_11740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRY30694.1"
                     /translation="MTTRARSMRFDQLLKGMACRRIGPAGNPLIRGVCYDSRRVRPGD
                     LFVALTGRKADGHDFIPQVAAAGAVAVLAERPVESPAPAALVPSTLEALSPVSLRFYG
                     HPSAGLCVVGVTGTNGKTTITYMLESIWKAAGRKSGVIGTVEYRWGKKTERATNTTPF
                     SADIQRLLARMKTDGVTHVAMEVSSHALELHRVDGMEFRAGIFTNLTRDHLDFHQTME
                     NYFEAKAKLFDRLLPTGKKRKFAIINADDAWAEKMLARVRCDRLTYGVRSPADLSAEN
                     VSLSAEGTSFDLAVAGRRVPVRLGSVGMHNVSNALAAAGAALSLGIPMSSVQKGLRSF
                     RGAPGRLERVVLPSKKRGVSSALSFSVFVDYAHTDDALKNVLEALRPLASGRLITVFG
                     CGGDRDRTKRPLMGDVAVRMSDYVIVTSDNPRSEEPQRIALDIEVGIRRTGLSHYEVV
                     LDREQAIRKAVGLAKPGDVVLTHCNGEPDAYGYPLRIWAYDQPESIGWYAEEAVVGDW
                     "
BASE COUNT          363 a          611 c          748 g          384 t
ORIGIN      
        1 cttttgaagc ccatcccgcg atggagcggg gtgagcctgc ccatcacctc cttcgggcag
       61 gaggtcagcg tcaccgccct gcaactggcc tgcgcctaca gcgccatcgc caacggcggg
      121 gtgctgctgg aaccgcggct gttcgtggag gcggtggacc agatcggtgg ccgaaagacg
      181 tgggaaacgc cgacggccat tcggcgggtg atatccgaaa agacggcggc caggctgagg
      241 gacatcttgc agggcgtggt gttgcggggc accgggaaag acgctttgct cgacgggtgg
      301 acggtggccg ggaaaaccgg cacggcccag aagatcgacc cgcgcacgcg gaagtattcg
      361 gcggacaaat tcgtggcttc cttctgcggt tttgtgccgg ctgtgaagcc ccggttgacc
      421 atcgtggtgg tgtatgacga acctcagggg gtctcctggg gcggctacaa cgccggtccc
      481 gttttccgca acgtggcgtg gcatgccatg acatatctgg gggtgtcgtc ggacaaggag
      541 acccgtctgg cgcgccggaa actgcgggag gtgtcgcctt tatgaccacg cgcgcgcgtt
      601 cgatgagatt cgaccagttg ctcaagggga tggcctgtcg acggatcggg ccggcgggga
      661 accccctgat ccgcggggtg tgttacgatt cccgccgtgt gcggccggga gatctctttg
      721 tggcgttgac ggggagaaaa gccgacgggc atgatttcat tccgcaggta gcggcggccg
      781 gggcggtggc ggtgttggcg gagaggccgg tggaatcgcc ggcgccggcg gcgctggtgc
      841 cgtccacgtt ggaggccctt tcgccggtct ccctccgttt ctacggccat ccgtccgccg
      901 ggttgtgcgt cgtcggcgtg acggggacca acggcaagac caccatcacc tatatgttgg
      961 aaagcatctg gaaggcggcg ggccgcaagt ccggcgttat cggcacggtg gaataccgct
     1021 ggggaaagaa gacggaacgg gccaccaaca ccacgccgtt ttcggccgac atccagcggc
     1081 ttctggcgcg catgaagacc gacggcgtga cacacgtggc catggaggtg tcgagccatg
     1141 cgctggaact gcatcgcgtg gacggcatgg agttccgggc ggggatattc accaacctga
     1201 cgcgagacca tctggatttc catcagacga tggagaatta tttcgaggcg aaggccaagc
     1261 tcttcgaccg gctgcttccg acgggaaaaa aaaggaaatt tgctataatc aacgccgacg
     1321 acgcgtgggc ggaaaagatg ctggcgcggg tccgttgcga ccgtttgaca tacggggtga
     1381 ggtcccccgc cgacctttcc gcggagaacg tctccctgag cgcggagggg acatcgttcg
     1441 atttggcggt ggcgggccgg cgcgtgccgg tccggttggg gagcgtgggg atgcacaacg
     1501 tttccaacgc gctggccgcc gccggcgcgg cgctgtctct gggcatcccc atgtcgtccg
     1561 ttcagaaagg tctgcgttcg ttccgaggcg ctccgggccg tctcgaacgc gttgtcctgc
     1621 cgtcgaaaaa acgcggcgtc tcctcggcgc tctccttcag cgttttcgtg gattatgccc
     1681 ataccgacga cgctttgaag aacgttctgg aggccctgcg gcctctcgcc tccggacggc
     1741 tgatcaccgt tttcggctgc ggcggcgatc gggacaggac gaaacgtcct ctcatgggcg
     1801 atgtggcggt tcggatgtcc gattatgtca tcgtcacatc ggacaacccc cgcagcgagg
     1861 agccgcagcg gatcgcgttg gacatcgaag tggggatccg gcggacggga ctctcccatt
     1921 acgaagtggt tttggacagg gaacaggcga tacggaaagc ggtggggctg gccaagccgg
     1981 gcgacgtcgt cctgacgcac tgcaacggcg agcccgatgc ctacggctat ccgctgcgga
     2041 tctgggccta cgaccagccg gaatcgatcg gctggtatgc ggaggaggcc gtcgtcggcg
     2101 actggc
//
LOCUS       DAOTFD010000325         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_45196_length_2106_cov_3.404681, whole genome shotgun sequence.
ACCESSION   DAOTFD010000325 DAOTFD010000000
VERSION     DAOTFD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45196_length_2106_cov_3.404681"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="P5079_11745"
     CDS             complement(<1..782)
                     /locus_tag="P5079_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30695.1"
                     /translation="MQKKHLPLLLAVLAFAVTFFVYWPAVHNGFVSWDDEANFLNNPA
                     YRGLGGTQLRWMWTTLHMGHYIPVTWMTLGFDYVLWGMDPRGYHLTNLLLHSLNAAFF
                     YLLVSWLFRKYASSSESAVMEPAALAAALFFSLHPLRVESVAWVTERRDLLAGVFFFG
                     ALAAYFKYVSLRRDGESAVFGEAGRSRRWYGVSVALFALALLSKATVLMLPVVLLLFD
                     LYPLRRLRREGDSPPKLTGGRLLIEKVPFLVLSLAAGVVLLAA"
     gene            complement(902..1489)
                     /gene="scpB"
                     /locus_tag="P5079_11750"
     CDS             complement(902..1489)
                     /gene="scpB"
                     /locus_tag="P5079_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRY30696.1"
                     /translation="MEVLLWMTDRPLKFSDFKDIVGSDCPPETELREELLAIGRELDA
                     RESPVQLMEIDGGYQLSSRPAFSPWIRRLYKDKTTLRLSTSALETLAIAAYKQPITRG
                     EIEEIRGVEVTGVLETLMERKLLKIVGRKETIGRPLLYGTTVDFMRQFGLKSLEDLPK
                     LEELLPAEEAAAAPAAPEPDAAPAAEPPPEEPSAR"
     gene            complement(1503..>2106)
                     /locus_tag="P5079_11755"
     CDS             complement(1503..>2106)
                     /locus_tag="P5079_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRY30697.1"
                     /translation="ATLMQIKARTLLPAPTTETGEEGPGPRAELINRLLEYQRYKEAA
                     KELEKRLQSHKDLHYRGSPIFAEEDYALDVSLFDLLDAFRDVLKELKPEIREVLYEEI
                     PLEVKIREILTYLEGRPFVTFREILKKETTRHALIVTFLAILELIRLKQISARQAEIF
                     GEIRIYRSEALAQISLIPETPAEPNPAPISPSEENNLGNQ"
BASE COUNT          421 a          627 c          673 g          385 t
ORIGIN      
        1 gccgccagca agacgacgcc cgccgccaac gagaggacga ggaacgggac tttttcaatc
       61 aacaatcgtc cgccggtcaa cttggggggc gaatcccctt cccgccggag ccggcgcaag
      121 gggtaaagat cgaaaagcaa caggacgacg ggcaacatca ggacggtcgc cttggacaac
      181 agcgccaaag cgaacaacgc cacggaaacg ccgtaccatc ggcgcgaacg gccggcctcc
      241 ccgaagacgg cggattcgcc gtccctccgc agggaaacat atttgaaata agccgccagc
      301 gcgccgaaaa agaaaacgcc ggccaaaaga tcgcggcgtt ccgtgaccca ggccaccgat
      361 tccacccgca gggggtgaag cgaaaagaac agcgccgccg ccagggcggc cggttccata
      421 acggcactct ccgacgacga tgcgtatttc cgaaagagcc acgacacgag cagatagaaa
      481 aaggcggcgt tcagggaatg caggaggagg ttggtgagat gatagcccct ggggtccatg
      541 ccccacagca cataatcgaa ccccaaggtc atccaggtga cggggatata gtgtcccatg
      601 tggagggttg tccacatcca tcggagctgt gttcccccca atccccggta cgcggggtta
      661 ttgaggaaat tggcctcgtc gtcccaactc acgaaaccgt tgtgcaccgc gggccaatag
      721 acgaaaaagg tgacggcgaa ggcgaggaca gcgaggagca gaggcaggtg ttttttttgc
      781 ataaggcggt ccggaagaga cggcggctca acggcccgtt ccggctctcc cgttggattg
      841 cggccgatcg gcatccgatg agaaatccgt caaccccggc ggcgttcgtc ttcgaaaaag
      901 ttcagcgggc ggacggttct tccggcgggg gttcggccgc gggagcggcg tcgggttcgg
      961 gagcggccgg cgcggcggcg gcttcttccg ccgggaggag ttcctccagt ttcggcagat
     1021 cctccaggga tttcagcccg aactgccgca tgaagtccac cgtggtgccg tagaggaggg
     1081 gccgtccgat ggtttccttg cggcccacga tcttcagcag ttttcgctcc atcagggtct
     1141 ccagaacgcc ggtgacctcc acgccgcgaa tctcctcgat ctcgccgcgg gtgatgggct
     1201 gcttgtaggc cgcgatggcc agggtctcca gggcggaggt ggagagccgc agggtggttt
     1261 tgtccttata caggcgtcgt atccaagggc tgaacgccgg ccgggaagag agctgatagc
     1321 ccccgtcgat ctccatcagc tggaccggcg attcccgggc gtccagttcc ctcccgatgg
     1381 cgagcagttc ctcccgcagt tccgtttccg gcgggcagtc gctgcccacg atgtccttga
     1441 aatcgctgaa tttcagaggg cggtccgtca tccacaagag gacctccaag attttttttc
     1501 ggctattggt ttccaagatt gttctcctcc gaaggggaaa tgggggccgg gttcggctcc
     1561 gccggcgttt ccgggatcaa cgaaatctgc gccaacgcct ccgaccggta gatgcggatc
     1621 tcgccgaaga tctccgcctg acgggcggaa atctgtttca gccggatcaa ttccaaaatc
     1681 gccaggaacg tgacgatgag ggcatgccgg gtggtctcct tcttcaggat ttcccggaac
     1741 gtgacgaacg gccgcccttc caagtacgtc aagatctccc ggatcttcac ctcgaggggg
     1801 atctcctcgt agaggacctc ccggatctcg ggtttcaatt ccttgaggac gtcccggaag
     1861 gcgtcgagga ggtcgaagag ggacacgtcc aacgcgtaat cttcctcggc aaagatcggg
     1921 gagccgcgat agtgcaggtc cttgtgcgac tgcagccgtt tttcgagttc cttggcggcc
     1981 tctttgtaac gctgatattc cagtaggcgg ttgatgagtt cggcgcgcgg gcccgggccc
     2041 tcttcgccgg tctcggtggt gggcgccggc aaaagggtgc gggccttgat ctgcatcagc
     2101 gtggcc
//
LOCUS       DAOTFD010000326         2082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_46029_length_2082_cov_3.738037, whole genome shotgun sequence.
ACCESSION   DAOTFD010000326 DAOTFD010000000
VERSION     DAOTFD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46029_length_2082_cov_3.738037"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5079_11760"
     CDS             complement(<1..591)
                     /locus_tag="P5079_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRY30698.1"
                     /translation="MKIKFGTSGWRAIIADDFTFHNVRIVTQSIVDYLRREGLHNRSV
                     VVGYDTRFLSEQFAQTAANMLAANGMHTLLCNRDTPTPVVAFEILRQKASAGINITAS
                     HNPYDYSGLKLNSAWGGPALPEITQRIEAGCEPYITGESHLKIPRDLTEVKKRLIVLS
                     DPQPHYFRHLERLVDTRALKRGKIRVAVDVLWGTGRG"
     gene            complement(666..>2082)
                     /locus_tag="P5079_11765"
     CDS             complement(666..>2082)
                     /locus_tag="P5079_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30699.1"
                     /translation="VGGWFLCLGVSVVLARFSVRFGGGRAGSERLIAPLRDVSEDGTS
                     SDILYADGSGKLHGRRAVLSALLPFRGGFWDRLVQRMQWSLFFHEDYHQRLRRAGRTP
                     WFEEIRAYLAQASATVDVFTGRSEQLWSDLNKAPFVRPLGLKRSLSRGWEFMLSVPAY
                     FAGRVVAVTNVGAFMAESAGRFFLWPGYSMAGLFKTASALPSELTPSRAAIGAALPAV
                     SALQETIRGRVWEAIAAQSLLVNPGMPGRGHAILVDGAGATVDAAGDLKLDRAVALTL
                     FQRLRALNDFYDGLSGTDRETLPLHVDLLLPQNLGLDQVQLRAAFKAILQTAGEVYPI
                     SEALLDRVVFNVRQVGEADHDAVVSLVREYAHSSLNTQVVTGRQDFWARVQSRVPEAS
                     LMIVLAGEIAVELARLEVEVVFTGAAAQALRRWIGEDDYRSLPAEVKDGENFRFKAMQ
                     LDPVKDLEDSDKKIRLFNIQA"
BASE COUNT          412 a          661 c          677 g          332 t
ORIGIN      
        1 cccccggccc gtaccccaca acacgtccac cgccacgcgg atctttcccc tcttcagagc
       61 ccgggtgtcc accaggcgtt ccagatggcg gaaatagtgc ggctgcggat cggagaggac
      121 gatgagccgt ttcttgacct ccgtcaggtc gcgcgggatc ttcaaatggg attcgccggt
      181 gatgtagggt tcacaaccgg cctcgatgcg ttgggtgatc tccggcaggg ccgggccgcc
      241 ccaggcggaa ttgagcttga gaccggaata gtcgtagggg ttgtggctgg cggtgatgtt
      301 gatcccggcg gaggcttttt gccggaggat ttcgaaggcc accaccggcg tgggggtgtc
      361 ccggttgcat agaagggtgt gcatgccgtt ggcggcgagc atattggccg ccgtctgagc
      421 gaactgttcc gaaagaaaac gggtgtcgta tcccacgacg acggagcggt tgtggagccc
      481 ttcgcggcgc agataatcca cgatggactg cgtgacgatg cggacgttat ggaacgtgaa
      541 atcgtcggcg atgatggcgc gccaaccgga agtgccgaat ttaattttca tggtagggga
      601 tggtagcaaa gcctcccggt cctgtcaacg gccaaaagcc gacggaaaat gcggaggcaa
      661 cgggatcagg cctggatgtt gaaaaggcgg attttcttgt cggaatcttc caagtctttg
      721 accgggtcga gctgcatggc cttgaagcgg aaattttcac cgtctttgac ctcggccggc
      781 aggctcctgt agtcgtcctc cccgatccag cggcgcagcg cctgggccgc cgccccggtg
      841 aacaccacct ccacctccaa ccgcgccagt tccacggcga tttcgccggc cagaacgatc
      901 atcagggacg cctcgggcac gcgggactgc acccgcgccc agaaatcctg gcggcccgtc
      961 accacctgcg tgttgaggga ggagtgagcg tattccctga ccaaggacac caccgcgtcg
     1021 tggtcggcct ctcccacttg gcgcacgttg aacacgaccc ggtccaacag ggcttccgaa
     1081 atcgggtaga cctcgccggc cgtttgcaaa atggccttga aagcggcgcg gagctggacc
     1141 tgatcgaggc cgagattctg cggcaacagg agatccacgt gcagcggcag cgtctccctg
     1201 tcggttcccg aaagaccgtc gtagaaatcg ttgagggccc gcagacgctg gaagagggtg
     1261 agcgccacgg cccggtcgag tttcaggtcg ccggcggcat ccacggtggc cccggcgccg
     1321 tccaccagga tggcatgacc tcggccgggc atgccgggat tgacgagcag ggactgggcg
     1381 gcgatggcct cccagacgcg gccacggatc gtttcctgca acgccgacac ggccggcagc
     1441 gcggcgccga tggcggcgcg ggatggcgtc agctccgacg gcagagcgct ggcggtcttg
     1501 aacagtcccg ccatgctgta gcccggccag aggaaaaacc gccccgcgct ctcggccatg
     1561 aacgccccca cgttggtcac ggccaccacg cgtcccgcga aataggccgg aacggaaagc
     1621 ataaattccc atccccgaga aagggaccgt ttcagaccga ggggccgcac gaaaggcgcc
     1681 ttgttcaggt cggaccagag ctgttccgaa cggccggtaa aaacatccac ggtggccgac
     1741 gcctgggcca gataggcccg gatttcctcg aaccacgggg ttcggcccgc ccggcggagc
     1801 cgttgatggt aatcctcatg gaagaacaac gaccactgca tccgctggac gagcctgtcc
     1861 cagaacccgc cgcggaaagg caggagcgcc gaaaggaccg cgcgtcggcc gtggagcttt
     1921 ccggaaccgt ctgcatacaa aatatccgag gacgtcccgt cttccgagac atcccgcaac
     1981 ggcgcgatga ggcgctcgga acccgcgcgg ccgccgccga accgcaccga gaaacgagcc
     2041 aagacgacgg aaacgcccag gcacaggaac cagcccccca cc
//
LOCUS       DAOTFD010000327         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_46297_length_2075_cov_4.085149, whole genome shotgun sequence.
ACCESSION   DAOTFD010000327 DAOTFD010000000
VERSION     DAOTFD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46297_length_2075_cov_4.085149"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5079_11770"
     CDS             <1..303
                     /locus_tag="P5079_11770"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30700.1"
                     /translation="GRKDPVYSAIHTMLSQYFLKRETVEPPKLVDGRWLMKQLHLPEG
                     PVIGRLLAAIREAQATGRVKTTAQALALAETVLKDRNIATLLERNEKNKTGGIPHD"
     gene            296..1063
                     /locus_tag="P5079_11775"
     CDS             296..1063
                     /locus_tag="P5079_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY30701.1"
                     /translation="MTETAPFSNMISALLLFTFSAALPFLLAVALSYGARRVALPWTV
                     FVTLVLFVSVALTTAFVLLTRGNFPPTYVGGLFLGNGLLIFLYYYETRGVNAVSQTEW
                     LVGGQNRGRIHLRHVLQGAIRLEEHGKSFYEDLALRLSDPDLRQLCLLLAREEEEHRL
                     HFQKYLTRWLAIPADEQTLAVLMDDLRQAGLFAKIDLPKSSEKDLLDYAVLQEEKTAE
                     FYQSLENLFPEAWKKLHIRQLVATERAHAERLRNFRR"
     gene            1419..1492
                     /locus_tag="P5079_11780"
     tRNA            1419..1492
                     /locus_tag="P5079_11780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1451..1453,aa:Gly,seq:ccc)
     gene            1883..>2075
                     /locus_tag="P5079_11785"
     CDS             1883..>2075
                     /locus_tag="P5079_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30702.1"
                     /translation="MKIVFTIAPYAWSELYPGAQSKQCGFRMSKKFGLIPGSTAPLGI
                     LYLSRMLKNAGHDVEFIDGV"
BASE COUNT          450 a          637 c          542 g          446 t
ORIGIN      
        1 ggccgcaaag accccgtgta ttcggccatc cacacaatgt tgtcgcagta ttttctcaaa
       61 cgcgagaccg tggaaccgcc caaactggtc gacggacgct ggctcatgaa acaactccac
      121 ctgccggagg gtcccgtcat cggccgcctg ctcgccgcca tccgggaggc ccaggccacc
      181 ggccgggtga aaaccaccgc ccaggccctc gcgctggccg agaccgtcct gaaagacagg
      241 aacatcgcca cgctcctgga gagaaacgaa aaaaacaaga ccggaggcat cccccatgac
      301 tgaaaccgca cccttttcca atatgatctc cgcgcttctc ctcttcacct tctccgccgc
      361 cctgccgttc ctcttggcgg tggcgctgtc ctacggggcc cggcgcgtgg ccttgccgtg
      421 gaccgtcttt gtcacgctcg ttctgttcgt ctccgtcgcc ctgaccaccg ccttcgtcct
      481 tcttacccgg ggaaacttcc cgcccaccta cgtcggtgga ttgttcctgg ggaacggcct
      541 ccttatcttc ctctactatt acgagacgcg gggagtgaac gccgtctctc aaacggagtg
      601 gcttgtcggc gggcaaaaca ggggacgcat ccatctccgc catgtgttgc agggagcgat
      661 ccgtctggaa gaacatggga aatccttcta cgaggacctc gcgcttcggc tctccgaccc
      721 cgatctccgg caactttgcc tgctcctggc gcgggaagaa gaggaacacc ggctgcactt
      781 tcaaaaatat ctcacgcgct ggctcgccat ccccgccgac gaacaaaccc tcgccgtctt
      841 gatggacgac ctccgacagg ccggcctgtt cgccaagatc gacctcccca aatcgtcgga
      901 aaaggatctt cttgactacg ccgtcctcca ggaagagaaa acggcggagt tctatcagtc
      961 gctcgaaaat ttattcccgg aagcatggaa gaaactgcac atccggcaac tggtcgccac
     1021 ggagcgcgcc cacgcggaac gcctgcggaa tttccggcgc taaccgcctc gaaggtattt
     1081 tcagggcctg tttgtcggaa aaagaaaagt ctgctaaaat gccatcgggg ttttcaagcc
     1141 tccggcttgc cgatggtccc aaagtgatcc gtgggacaat aaaaccggtt tggaatgcgg
     1201 gtgccccctg aggagttgtc gtcgctctgc gacgcctcag gtcccaaagt gccacgtggg
     1261 acaacaggtt gcgatgcaaa tttcaatttg acaattgcgg gtgccccgac tcacatactc
     1321 ggctgaaatt gggactgaaa tttcagcctg ttcctcgggg ccaccaagta acaacataat
     1381 ttcaggcggc ctaaaggccg cccagtgtca acatagttgc gggtgtagtt caatggtaga
     1441 atggaagctt cccaagcttc agacgtgggt tcgattccca tcacccgctc cactttccgt
     1501 ccgccttgtg ttgggccact aatgcaaatg gtggttcgac gataaggcgg atttttttgc
     1561 ccaaaatctg acggttcgag cttggttttc aatgccgcta ggcatagtcc cggtttgtcg
     1621 gatggaatga agcgacgcgg ccagcgaccg cgctagtgga agtgccgtgt ggccggccga
     1681 ggcgaaaatc gccgccgaaa acctgtttat taaggtcttt cgacccattg acgtcccttt
     1741 ctgcaattta cttttcatat tgtcgggact gtcccaaaca attttgtaaa aaggagaaag
     1801 ggacgaaatt taccaaaggt tcgagccgct catttccggg cctgtttgaa gccccttcag
     1861 aaaactggca ttattctttc ctatgaaaat cgtgttcacc atcgcgccct acgcctggag
     1921 cgaactctat ccgggcgccc aatccaaaca gtgcggtttc cgaatgagca aaaaattcgg
     1981 gctcatcccc ggatccaccg cgccgctggg catcctgtat ctctcccgca tgctgaaaaa
     2041 cgcgggacac gacgtcgaat tcatcgacgg ggtca
//
LOCUS       DAOTFD010000328         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_47321_length_2049_cov_3.620863, whole genome shotgun sequence.
ACCESSION   DAOTFD010000328 DAOTFD010000000
VERSION     DAOTFD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47321_length_2049_cov_3.620863"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5079_11790"
     CDS             <1..441
                     /locus_tag="P5079_11790"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HRY30703.1"
                     /translation="LSSIAYLEHLLVTEGPLTRRPPDGDQKKDIAFGFQTAKAVAGLD
                     LGQTVCVKNQAVLAVEAMEGTDACIRRAGTLCDGSFTVVKVAKPRQDLRFDVPVIGAG
                     TLQALQEANAAVLAVEAGKTLFFDKAHFLTEADKTGLVLVGTKC"
     gene            435..1439
                     /locus_tag="P5079_11795"
     CDS             435..1439
                     /locus_tag="P5079_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY30704.1"
                     /translation="MLRRLFSRKKTPVPPRPVLPDAERLAVGIVGVGHLGQHHVRLLA
                     GIPEAKLVGITDLNMDRARSLAVQHGTRAFERAEDFLPQVKAVVIATPTPTHHALAKK
                     FLQAGHHCFVEKPLTERVAEAEEVIALARANNLVLQVGHVERFNPAVEEMIRRAQNPL
                     FIEANRLGPYDPRVAHVSVVLDLMIHDIDIVLAMVKDKVVKFDAVGAKVFSDQEDIVK
                     VTLHFSRGCRADLSASRVSLQRFRKIRVFQKDAYMSLDYSERSLKVYRKKSAVIRNLL
                     DVAVEHPKLEKKDPLESELKHFVQCVKDGKPPLVGGEHGRDALELALEIRRAAQVHSL
                     "
     assembly_gap    1623..1632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1633..>2049
                     /locus_tag="P5079_11800"
     CDS             <1633..>2049
                     /locus_tag="P5079_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRY30705.1"
                     /translation="DTEILALGGPALKAVADHFLADLVSQSVMGFWEPLKKIPWFWRV
                     HKEILLPALRDFQPGVVVPVDSFGFNRFTAQAAHRAGRKVYYFISPQVWASRPWRINI
                     LRKNVDKMLTIFPFEEKLYKKHDIHTAFVGHPLLDVL"
BASE COUNT          400 a          653 c          628 g          358 t
ORIGIN      
        1 ctgtcctcca tcgcctatct ggaacatctg ctcgtcacgg aaggccctct gacgcgacgg
       61 ccccccgacg gcgaccagaa aaaggacatc gcctttggat tccaaaccgc caaagccgtg
      121 gccggactcg acttgggcca gacggtctgc gtgaagaacc aagcggtgct ggcggtggaa
      181 gccatggagg ggaccgacgc ctgcatccgc cgcgccggca ccctttgcga cgggtctttc
      241 accgtggtga aggtggccaa acccagacag gatctccgtt tcgacgtgcc cgtcatcggg
      301 gcgggaacgc tccaggccct ccaagaggcc aacgccgccg tgctggcggt ggaggcgggg
      361 aaaaccctct ttttcgacaa ggcccatttt ctgacggagg ccgacaaaac gggactcgtc
      421 ctggtcggca cgaaatgctg agacgcctct tctcccgaaa gaaaacgccg gtcccgcccc
      481 gtcccgtcct gccggacgcg gagcggctgg ccgtcgggat cgtcggcgtc gggcacctgg
      541 gccagcacca cgtgcggctg ctggccggca ttccggaagc caagctcgtg ggcatcaccg
      601 acctgaacat ggaccgagcc cggtcgttgg cggtccagca cgggacccgc gccttcgagc
      661 gcgccgagga tttcctgccg caggtcaagg ccgtggtcat cgccaccccc acgccgacgc
      721 accacgcgct ggcaaaaaaa ttcctccagg cggggcacca ttgtttcgtg gaaaaacccc
      781 tcaccgaacg ggtggcggag gcggaggagg tcatcgccct cgcccgggcg aacaatctcg
      841 tgctccaggt gggccacgtg gaacggttca acccggcggt ggaggagatg atccgccggg
      901 cccaaaaccc gctcttcatc gaggccaacc ggctgggtcc ctacgacccg cgcgtggccc
      961 acgtcagcgt ggtgctggac ctgatgatcc acgacatcga catcgtcctg gccatggtga
     1021 aggacaaggt ggtcaagttc gacgccgtgg gcgccaaggt tttctccgac caggaagaca
     1081 tcgtcaaggt caccctgcac ttttcgcgcg gctgccgcgc cgacctctcc gccagccggg
     1141 tgagcctgca gcgcttccgc aagatccgcg tgttccagaa agacgcctac atgtcgctcg
     1201 actattccga gcgcagcctg aaggtctacc gaaaaaaaag cgccgtcatc cggaacctgc
     1261 tggatgtggc cgtggaacat cccaaactgg aaaaaaagga ccccctggaa tcggaactga
     1321 aacatttcgt ccagtgcgtg aaggacggga agccgccgct ggtcggcgga gaacacggcc
     1381 gagacgccct ggagttggcg ctggagatcc gccgggccgc ccaagtccac tccctatgac
     1441 cccacgttcg tggggctttt cgccaaagcg gtgggttcca tcggaagcgc ggccgaccgc
     1501 ccaggggacg atgaatttac tcccgcccct tttggaaaaa tgggcatgaa catgcccccc
     1561 cgcgttctgt tcgtggccgg ggacctctcc ggggaccaaa acgccgccca ggtggcccgg
     1621 cgnnnnnnnn nncgacaccg aaatcctggc gctgggggga ccggccctca aagccgtggc
     1681 ggaccatttt ttggcggact tggtgtccca aagcgtcatg gggttctggg aacccctgaa
     1741 aaagatcccc tggttttggc gcgttcataa agagatttta ctccccgcgt tgcgggattt
     1801 tcagccgggg gtggtggttc cggtggactc gttcggcttc aaccggttca cggcgcaggc
     1861 ggcccatcgg gccggtcgaa aggtctacta tttcatcagc ccgcaggtct gggccagccg
     1921 gccgtggcgg atcaatattc tcaggaaaaa tgttgacaaa atgttgacta tattcccttt
     1981 tgaagaaaaa ctttataaaa aacatgatat ccatacggcc ttcgtggggc accctctgtt
     2041 ggacgtcct
//
LOCUS       DAOTFD010000329         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_48882_length_2009_cov_4.062436, whole genome shotgun sequence.
ACCESSION   DAOTFD010000329 DAOTFD010000000
VERSION     DAOTFD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48882_length_2009_cov_4.062436"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1351)
                     /locus_tag="P5079_11805"
     CDS             complement(53..1351)
                     /locus_tag="P5079_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRY30706.1"
                     /translation="MKKLQKLIETRRAGIGIIGVGYVGLPLAVAFAREGFHVVGFDVD
                     ASRVRALNSGRSYVPDVPSSAVRELRRTGGFAASGDFRELRKMDVIVICVPTPLRKTK
                     DPDISYIVAALEKIARVLRRGQLVVLESTTYPGTTREVALPLLRKSGLTVGRDFWLAF
                     SPERVDPGNKRYGIRNTPKIVGGITPDGAALAESLYSTVVEKIVRVSSTECAEMVKLL
                     ENTFRAVNIGLVNEVALMCDRLKLDTWEVIAAAATKPFGFMPFYPGPGLGGHCIPLDP
                     HYLAWKLKTLNFYARFIELAGEINSQMPEYLVRKIGDALNERGKSVKGAKVLVLGVAY
                     KRDIGDVRESPALDVIELLKKKQAVVHYHDPYVPRLTAGSLSLKSLPLTDALLRRQDC
                     VVVTTSHSCFDMRRVVARSRLVVDSRNATAGLPARHVVKL"
     gene            1560..>2009
                     /locus_tag="P5079_11810"
     CDS             1560..>2009
                     /locus_tag="P5079_11810"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRY30707.1"
                     /translation="MVKITHIEATPNPHALKFIVDGRLLETGAKSYASPAAVGDDALA
                     RALFSVGKEVRSVFYMDRFVTVTKSPEADWKDLQSRVVEAVETAAQPLAPGDPSEKTE
                     FPADGGTALSTINQVLDAHIRPYLANDGGGLEVIDYRDYVLTVRYQGA"
BASE COUNT          368 a          675 c          605 g          361 t
ORIGIN      
        1 tccggcggcg ccggtgacga gaatcgtttt tttcatacgg tcggacgggc ccctacagtt
       61 tgacgacgtg ccgggcgggc aagcccgcgg tggcgttgcg cgaatccacc accagccgcg
      121 aacgggccac cacccggcgc atgtcgaaac aactgtggct ggtggtgacg acgacgcagt
      181 cctgccggcg caggagggcg tcggtcaaag gaagggattt cagggagagg ctccccgccg
      241 tcaaacgcgg cacatagggg tcgtggtaat gcaccacggc ctgtttcttt ttcagcaatt
      301 cgatgacgtc cagcgcgggg gattcgcgca cgtccccgat gtcgcgcttg taggcgaccc
      361 ccaacaccag gaccttcgcc cccttgaccg actttcccct ctcgttcagc gcgtcgccga
      421 tcttgcggac gaggtattcc ggcatctgcg aattgatctc gccggccaat tcgatgaagc
      481 gggcgtagaa gttcaacgtc ttcaatttcc atgccaggta atgcgggtcc aacgggatgc
      541 aatgcccgcc caggccgggg ccggggtaga acggcatgaa cccgaacggt ttggtggcgg
      601 ccgcggcgat gacctcccag gtgtcgagtt tcagccggtc gcacatcagg gccacctcgt
      661 tgaccaatcc gatgttgacg gcccggaacg tgttctccag gagtttcacc atctccgcgc
      721 attcggtgga cgacacgcgc acgatcttct ccaccaccgt ggaatagagg gattccgcca
      781 gggccgcgcc gtcgggggtg atcccgccca cgatcttggg ggtattgcgg atgccgtacc
      841 gtttgttgcc ggggtccacc cgttccggag aaaaagccag ccaaaaatcc cgtccgacgg
      901 tcagtccgga ttttctgagc agcgggaggg cgacctcgcg ggtggtcccc ggataggtgg
      961 tgctctccag caccacgagc tgtcccctcc ggagcacccg ggcgatcttt tccagcgccg
     1021 cgacgatgta actgatgtcc gggtccttgg ttttgcgcag cggcgtgggg acgcagatga
     1081 cgatcacgtc catcttcctc agttccctga aatcgccgga ggcggcgaaa ccccccgtcc
     1141 gccgaagctc ccgcaccgcc gaagaaggca cgtccggaac gtaggagcgg ccggaattca
     1201 gggcgcgcac gcgagaggcg tccacgtcga agccgacgac gtggaaccct tcccgggcga
     1261 acgccaccgc cagcggcagg ccgacatagc cgacgccgat gatgccgatc ccggcgcggc
     1321 gcgtctcaat gagtttctgc aattttttca taccgcctcc cgttttccgg cctttcccgt
     1381 tgaaccacga gcccgtcggt ccaccaccgc ccctccgcct ccgaggatcc cgcggggggc
     1441 gccgaacagc cgacagacgc cccggcggga agagtcattc tatttcactt tcgtgaggat
     1501 gcgcaaccgc ccgcccgatt atacttgact gttttagtca gatatattaa attcattcta
     1561 tggtcaaaat cacccacatc gaagccacgc ccaacccgca cgccctgaaa ttcatcgtgg
     1621 acggccgcct cctggaaacc ggcgccaaat cctacgcgtc cccggcggcc gtcggggacg
     1681 acgccctggc ccgcgccctc ttttccgtcg gcaaagaggt gcggtccgtt ttctacatgg
     1741 accggttcgt caccgtcacc aagtcgcccg aagccgactg gaaagacctt cagtcccgcg
     1801 tggtggaggc cgtcgaaacc gccgcccagc ccctcgcccc cggagacccc tccgaaaaaa
     1861 cggaattccc ggcggacggc gggacggccc tttcgaccat caaccaggtc ctggacgccc
     1921 acatccggcc gtacttggcc aacgacggcg gcgggttgga ggtcatcgac tatcgcgatt
     1981 acgtcctgac ggtgcgttat cagggagcc
//
LOCUS       DAOTFD010000330         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_49861_length_1986_cov_3.696530, whole genome shotgun sequence.
ACCESSION   DAOTFD010000330 DAOTFD010000000
VERSION     DAOTFD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49861_length_1986_cov_3.696530"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1986)
                     /locus_tag="P5079_11815"
     CDS             complement(<1..>1986)
                     /locus_tag="P5079_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30708.1"
                     /translation="RRNDAITGVVTGETGKGRLIVLVTGGFHTPGLTQKLREQGVSYA
                     VVCPRVQKIEELNALEVFTRGHTPLDRVLLGEKIFLADPLTVGSRPMNAEQAETQTVQ
                     REMWGALEAVFGEPLDAINARLSAGRLSPLRKITETGERRMLGNLEIVSLLLHVDGSP
                     EPLALKLAVRKGELEPGAAKMLDGLADDHVRLTSREGEESFSMGETTVEIWASRSGWA
                     TRFASEAFYRQVLDALLDILKKRLPVVASLDAGEPVELVQMLAGKTAEGLVAGTLPFA
                     KVSGVLKGRFDEENIPEFLTDFASRAAKGLEDDLRKFGLGLPELFVGLLEIFLEPRKV
                     QRLEPELEALRQELLAAFNADRSGTESERLGILLPALGIKSIDVQVYEGTELVEDGQR
                     YLLSPLIVRSDGVAVFYINRALLETIQKQDGKKRAELVSALVEQKLVLQKYVRQNRPF
                     EVARADARAISDAQKELLTVADQAVAALLQAKRGPEQALKDVLQSIELELNSDQEEGS
                     PTREVLVLLKKHILAKGQLPTEAQMWKMMKGVAVRKNTPYDPFDLFVVYKAVLHGLVS
                     PPKGLWDDPELRVAVLAQLVQTMGIPSAQLLKEEHLKLMEEKFYLQDMFEGHSYKELI
                     QELLTRENLEKGVLPLRARFLDEQGNVRSEYAALNEEL"
BASE COUNT          396 a          646 c          530 g          414 t
ORIGIN      
        1 agctcctcgt tcagggcggc gtattcgctc cggacattgc cctgttcgtc caaaaagcgg
       61 gcccgcagcg gcaacacgcc cttctccagg ttctccctcg tcaacagttc ctgtatcaac
      121 tctttatacg agtgcccttc aaacatatcc tgcaagtaaa atttttcttc catgagcttc
      181 aggtgttctt ccttcaacaa ctgcgcggaa ggaatcccca tcgtctgaac aagctgggcg
      241 agcacggcca cacgcaactc cggatcatcc cagagccctt tcggtgggga gaccagcccg
      301 tgcaggaccg ccttatacac cacaaaaaga tcgaacgggt catagggggt atttttccgg
      361 accgcaacgc ccttcatcat cttccacatc tgggcttcgg tgggcaattg tcccttggcc
      421 aaaatatgtt tcttcaagag gacgagcact tcgcgtgtgg gactgccctc ttcctgatcg
      481 gagttcaatt ccagttcgat ggactggagg acgtctttca gcgcctgttc cgggccgcgt
      541 ttggcctgaa ggagggcggc gacggcctgg tcggcgacgg tcagcagttc tttttgggcg
      601 tcgctgatgg cccgggcatc ggcacgggca acctcgaaag gacggttttg gcggacgtat
      661 ttctgcaaca ccaatttctg ttccacgagg gccgaaacca gttccgccct cttttttccg
      721 tcctgtttct gaatggtttc cagcaacgcc ctgttgatat agaacaccgc gacaccgtcg
      781 gaacgaacga tcaagggaga caacagatat cgttggccgt cctccaccag ttccgtcccc
      841 tcatagacct ggacgtcgat ggatttgatg cccaaggcgg gaagaaggat ccccaacctt
      901 tccgactccg ttccggagcg atccgcgtta aacgccgcca ggagttcctg ccgaagggcc
      961 tccagttccg gttccaaacg ttggaccttc ctcggttcca aaaatatctc caggagaccg
     1021 acgaacagtt ccggcagccc gagaccgaat ttccgcaaat cgtcttccag tcctttcgcc
     1081 gctctggagg cgaagtccgt cagaaattca gggatattct cttcgtcaaa ccggcccttc
     1141 aagacgccgg agacttttgc aaagggaagc gttccggcca ccagcccctc cgcggttttt
     1201 ccggcgagca tctggaccaa ctccaccggt tcgccggcgt ccagggaggc gacgaccggc
     1261 agacgtttct tcagaatatc gaggagggcg tcgaggacct gccgatagaa cgcctcggaa
     1321 gcaaaacgcg tcgcccagcc ggaacgggac gcccatatct ccacggtcgt ctcgcccatc
     1381 gagaaggact cctctccctc gcgcgacgtc aaccgcacat gatcgtcggc caggccgtcc
     1441 aacattttag cggcccccgg ttccagttct cctttgcgca ccgccagttt cagcgccaga
     1501 ggctccggcg aaccgtcgac gtggaggagg agggagacaa tttccaaatt acccaacatc
     1561 cggcgctctc ccgtctccgt gattttcctc aacggcgaca gacggcccgc gctcagccgc
     1621 gcgttgatcg cgtccaaggg ctcgccaaaa accgcctcca acgcgcccca catctctctc
     1681 tggaccgtct gcgtctccgc ctgctcggcg ttcatcgggc gggagccgac ggtgagcgga
     1741 tcggccaaaa atattttttc gcccaaaagg acgcgatcca ggggcgtgtg gccccgggtg
     1801 aacacctcca acgcattcag ttcttcaatc ttctgcacgc ggggacaaac caccgcgtaa
     1861 gacacgccct gttcccggag cttttgggtg aggccgggcg tatggaatcc ccccgtgacc
     1921 aacacgatca gccggccctt gcccgtttcg ccggtcacga cgccggtgat ggcatcgttc
     1981 cggcgc
//
LOCUS       DAOTFD010000331         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_51119_length_1957_cov_4.457939, whole genome shotgun sequence.
ACCESSION   DAOTFD010000331 DAOTFD010000000
VERSION     DAOTFD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51119_length_1957_cov_4.457939"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="P5079_11820"
     CDS             complement(<1..1188)
                     /locus_tag="P5079_11820"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRY30709.1"
                     /translation="MIRTRLSFPRLASEARCRSNRRRTTPRIRLGLERLRPVLTRLSH
                     PEDDFASLLIAGTNGKGSVAALLENVLRTAGYRTGLYTSPHLVSPAERVRVNGTNIAG
                     AEWKRLLRQVADAAGRSRQELTEFESQTLAAYLHFQNEKVDIAVVEVGLGGRLDATNT
                     LPAPEATVITSIGHDHLSWLGPTLRDVLHEKMGILRHGVPHFQSLPPSLDPRSSVGPA
                     LSSTLNRDILVKGKHVDWVNRMQVFSVSLPHGTYKNLKIHLLGRHQLNNAALAVAVLD
                     SLKRRGWNISDSHLRRGLLRASWPGRFQVFNARPGGRPRTLLDGAHNPEAMDSLAAAY
                     RASPWGKESATLIFGCLKDKDANGLIERLRPLVRRVFTVPLPTTRSRSARTLARMWSR
                     FRPA"
     gene            complement(1185..>1957)
                     /gene="accD"
                     /locus_tag="P5079_11825"
     CDS             complement(1185..>1957)
                     /gene="accD"
                     /locus_tag="P5079_11825"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRY30710.1"
                     /translation="KELGDNLKVCPKCGHGMRMSARERIPLFFEPDSFKEFAADLQPV
                     DILGFVDTAPYKKRLKDNLKKTKLKEAAVAGEGLMGPYRVACALLDFEFMGGSMGSVV
                     GEKVTLAIEHALKENIPLVVVSSSGGARMQEGIVSLFQMAKTSAALARLRKARLPFIS
                     VLADPTTGGVTASFATLGDVIIAEPKALIAFAGPRVIEQTIRQTLPQGFQHSEFLLKH
                     GMVDMVVERKDLKKTIVSLLKVFMSSRSKNKSSTAKAE"
BASE COUNT          356 a          545 c          681 g          375 t
ORIGIN      
        1 ggccggccgg aaccgagacc acatccgggc gagagtgcgg gccgaacggc tgcgcgtggt
       61 cggcagaggg accgtaaaga cccgccggac caggggccgg aggcgttcaa tgagtccgtt
      121 ggcatctttg tctttgagac aaccgaaaat gagcgtggcg ctttctttgc cccagggaga
      181 cgcccggtag gcggccgcga gggagtccat cgcttccgga ttgtgggcgc catccaaaag
      241 agtgcgagga cgaccccccg gccgggcgtt gaagacttga aaccggcctg gccaggaggc
      301 ccggagcagc ccccgccgga gatgtgaatc ggagatattc cagccgcgtc gcttcaggga
      361 atccaggacc gccaccgcca aggcggcgtt gttcagctga tgccgtccca ggagatgtat
      421 tttcaagttt ttatacgtcc cgtgcgggag agagacgctg aaaacctgca tccggttcac
      481 ccagtccacg tgttttcctt tgacgaggat atcccggttg agcgttgagg agagggccgg
      541 gccgacggag gaacgagggt cgagcgacgg cggcagggat tggaaatgcg ggacaccgtg
      601 ccggaggatg cccattttct catggaggac gtcccgcagg gtggggccca gccaggagag
      661 atggtcgtgg ccgatggagg taatgacggt ggcttccggg gcggggagcg tgttggtggc
      721 gtccaggcgt ccgccgagcc cgacttcgac gaccgcgatg tccacttttt cgttttgaaa
      781 atggagataa gccgccaacg tctgggattc gaattcggtg agttcttgtc gggatcggcc
      841 ggcggcgtcg gccacttgcc gcaaaagacg tttccattcg gcgccggcga tgttggtccc
      901 attcacgcgc acgcgttcgg cggggctcac cagatgcgga gacgtataaa gtcccgtgcg
      961 gtaaccggcg gtgcggagga cgttttccag aagggcggcg acggagcctt tcccgtttgt
     1021 gccggcgatg aggagggagg caaaatcgtc ctcggggtgg gagaggcgcg tcaggaccgg
     1081 ccggaggcgt tccaagccca ggcggattct cggcgtggtc ctgcggcggt tgcttcggca
     1141 gcgtgcctcc gaagcgaggc gcgggaaaga gaggcgggtg cggatcattc cgctttggcc
     1201 gtcgacgatt tgttttttga acgggaggac atgaacactt tcagcaagga gacgatcgtt
     1261 tttttcaagt cctttcgttc caccaccatg tccaccatgc cgtgtttgag gagaaactcc
     1321 gagtgttgga agccctgggg cagggtttgg cggatggtct gttcgatgac gcgcgggccg
     1381 gcgaaggcga tgagggcctt gggctcggcg atgatgacgt ctcccaaggt ggcgaaggag
     1441 gcggtgacgc cgccggtggt ggggtcggcc aacacggaga tgaacggcag ccgcgcttta
     1501 cggaggcggg ccagggcggc gctcgttttc gccatttgaa agagcgagac gatcccttcc
     1561 tgcatccgcg ctccgccgga ggaggagacc accaccagag ggatgttctc cttcagggcg
     1621 tgttcgatgg ccagcgtgac tttctcgccg accacgctcc ccatgctgcc gcccatgaac
     1681 tcgaaatcca aaagggcgca ggccacgcgg tagggaccca tcaacccttc ccccgccacg
     1741 gccgcctctt tcagtttggt tttcttgaga ttgtccttga ggcgtttttt gtacggggcg
     1801 gtatcgacga aaccgaggat gtccaccggc tgaagatccg ccgcgaactc cttgaatgaa
     1861 tccggctcga agaagagcgg gatgcgttcc ctggcggaca tccgcatgcc gtgtccgcat
     1921 ttggggcaga ccttcagatt gtcgcccagc tccttgt
//
LOCUS       DAOTFD010000332         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_53086_length_1913_cov_3.572121, whole genome shotgun sequence.
ACCESSION   DAOTFD010000332 DAOTFD010000000
VERSION     DAOTFD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53086_length_1913_cov_3.572121"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1913)
                     /locus_tag="P5079_11830"
     CDS             complement(<1..>1913)
                     /locus_tag="P5079_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30711.1"
                     /translation="IYVEDTTYFPPSGTVDVESELNITYTGKTATSLIGVTRSAPVSH
                     PSNVLAKGPRRNKTYYVVYDIDELATVGALVGKQTELGMTLVSGSYMTVGAPDIVSNT
                     NLPFTASILDINEYADTVTVTPFNPDNTSPTHGDETLQQRATAQVVLGFTLETNQSEA
                     TWQGVRIRSTGTMVSELDISTVTIWVDGNRNSFFDPAAGYDVPIGTGSFGNIGETGVA
                     QVLFGASAPTLVTAVRAEAEGLPSRYFVTYDISPTAVPDRMAGARLAGAADFIVTAPN
                     TVSPANVTPFNGKLRQIIPAPQNVKCVFTPLFSSLQGTTTMQSLVSPTLDGNISSTVD
                     IIPVLNVTDVPSHGYAVIDSEIIYYSTVGAASLQGVLRGQLNTTPAPHTDDTLVGIQY
                     TQSDKNVAFLKMGVYVDDPLGYNVRWYNFKINRYQPVGLNGSDADVGMVRIYRDTGQP
                     GLDRNPATGLVNDALIAQKKFGDGESSAQATLTLDGHLFGGGTDYILITPTTQYYIIT
                     MDIDPTATFDHVVGARVPSSTNLTIGAQTPNDGIHTVLLDNFPGLSPTAVIRATEDTL
                     QVRAESLMPSTVVQNQKNVPVLRLNLRSDVNTVVWQKIKLDLMTTNGAVDGDVQLINL
                     YRVRADCRGQKEE"
BASE COUNT          288 a          551 c          695 g          379 t
ORIGIN      
        1 tttcttcctt ttgtccccgg caatcagcac gaacgcggta gaggttgatc agctgcacgt
       61 cgccgtccac cgcgccgttg gtggtcatga ggtccagttt tatcttctgc cacacgaccg
      121 tgttcacgtc ggagcgcagg ttcagccgca ggacgggcac gttcttctgg ttctgcacca
      181 cggtgctggg catgaggctc tcggcgcgca cctgcagggt atcctccgtg gcgcggatga
      241 ccgcggtcgg ggaaagtccg gggaaattgt ccagcagcac ggtgtggatg ccgtcgttgg
      301 gcgtctgcgc gccgatggtg aggttggtgg agctgggcac gcgggcgccc accacgtggt
      361 cgaaagtggc ggtggggtcg atgtccatgg tgatgatgta atactgggtg gtgggggtga
      421 tgaggatgta gtccgttccg ccgccgaaaa gatggccgtc cagcgtgagg gtggcctggg
      481 cggaggattc gccgtcgccg aacttcttct gcgcgatgag ggcgtcgttc accaggcccg
      541 tggcggggtt gcggtccaag ccgggttggc cggtgtcgcg gtagatgcgc accatgccca
      601 cgtccgcgtc gctgccgttg agccccaccg gctggtagcg gttgatcttg aagttatacc
      661 accgcacgtt gtagcccagg gggtcgtcca cgtacacccc cattttcagg aaggcgacgt
      721 tcttgtcgct ctgggtgtac tgaatgccca ccaacgtgtc gtcggtgtgg ggggccgggg
      781 tggtgttcaa ctggccgcgc agcacgccct gcagggacgc cgccccgacg gtggagtaat
      841 agatgatctc gctgtcgatc acggcgtagc cgtgggacgg cacgtcggtc acgttgagca
      901 cggggatgat gtcgaccgtg gacgagatgt tgccgtccag cgtgggcgac accaggctct
      961 gcatggtggt ggtgccttgc aggctggaga acagcggggt gaagacgcac ttcacgttct
     1021 gcggcgccgg gatgatctgc cgcagtttcc cgttgaacgg cgtgacgttc gccggggaca
     1081 ccgtgttggg cgcggtgacg atgaaatccg ccgccccggc caggcgcgcc ccggccatac
     1141 ggtccggcac ggccgtggga gagatgtcgt aggtgacgaa gtagcggctg gggaggcctt
     1201 ccgcctcggc ccgcaccgcc gtcaccagcg tgggagcgct ggcgccgaag agcacctgcg
     1261 ccacccccgt ttcgccgatg ttgccgaagg acccggtgcc gatgggaacg tcgtatcccg
     1321 ccgccggatc gaagaagctg ttccggttgc cgtccaccca gatggtcacg gtgctgatgt
     1381 ccagttccga caccatggtg ccggtgctgc ggatccgcac gccctgccag gtggcctcgg
     1441 actggttggt ctccagcgtg aaacccagca ccacctgggc ggtggcccgc tgttgaagcg
     1501 tctcgtcgcc gtgggtgggc gaggtgttgt ccgggttgaa cggcgtcacg gtgacggtgt
     1561 cggcgtattc gttgatgtcc aggatgctgg cggtgaacgg caggttggtg ttggagacga
     1621 tgtcgggcgc gcccacggtc atataagagc cggacacgag cgtcatcccc aattcggtct
     1681 gtttgcccac cagcgcgccg acggtggcga gctcgtcgat gtcgtagacc acgtaatagg
     1741 tcttgttgcg ccgcgggccc ttggccagca cgttggacgg atgggacacg ggcgcggagc
     1801 gggtcacgcc gatgaggctg gtggcggtct tgccggtgta ggtgatgttc aattcgctct
     1861 ccacgtccac ggtgccggaa ggcgggaaat acgtggtgtc ctccacgtag atg
//
LOCUS       DAOTFD010000333         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_54118_length_1892_cov_4.164943, whole genome shotgun sequence.
ACCESSION   DAOTFD010000333 DAOTFD010000000
VERSION     DAOTFD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54118_length_1892_cov_4.164943"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="P5079_11835"
     CDS             <1..871
                     /locus_tag="P5079_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30712.1"
                     /translation="RISVRGVTDADSIGPAFLLFLPWLFGIRRRLPKVLLAVGLTLWF
                     VPAVFSGIARFRLPALAVFALLIGSALEEEFSWSPRKKTAYALLFLAALANFIWGSLT
                     LFSFGGWEVAFGLKSKSDYLNHPRKGYPTPYYSAAEFVNKAAPPTGRVLLIGEARGYY
                     LKKPFIAASVFNTQPMVSIANASPTPDDLYERLQAMGITHILVNAAETLYLHKNWPEK
                     FSAAGRETFDAFWQKHALPVFIDAGPGPSDFRRVAVFELVPDREALQPLRPRIPNPLL
                     EILDGGNPGIELP"
     gene            1202..>1892
                     /locus_tag="P5079_11840"
     CDS             1202..>1892
                     /locus_tag="P5079_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRY30713.1"
                     /translation="MSLENTTPPITANLPALDTGDTAWVLVSAALVLLMTPGLAFFYG
                     GLVRKKNVLSVLMQCFMLMCLISIQWVMLGYSLSFGPDIKGLIGNLDWFGLKNVGLAP
                     FPDYSATVPHQAFMIYQAMFAIITPGLILGAFAERMKFSAFCLFSLLWTTLVYDPVAH
                     WVWGIGGFLRTGGALDFAGGTVVHVNAGVAALAAALVLGKRQGYPDTMSPPHNLPFAV
                     LGAGLLWFGWFG"
BASE COUNT          331 a          633 c          509 g          419 t
ORIGIN      
        1 gcggatatcc gtacggggcg tgacggacgc cgattccatc gggccggcgt tccttctttt
       61 cctgccctgg ttgttcggaa tacgccgccg gctccccaaa gtcctcctcg ccgtgggcct
      121 gacactgtgg ttcgttcccg cggtgttctc cggaatcgcc cgtttccgcc tccccgccct
      181 ggcggtcttc gccttgctga tcggctcggc gttggaagag gaattctcct ggtccccgag
      241 gaaaaaaacc gcctatgcgc tcctcttcct ggccgccctc gccaatttca tctgggggag
      301 tctcactctt ttttctttcg ggggatggga ggtcgccttc ggattgaaat cgaaatccga
      361 ttacctgaac catccccgca aaggctatcc aacaccgtat tacagcgccg ccgaattcgt
      421 caacaaggcg gcgccgccga ccggccgggt cctcctcatc ggcgaagccc gcgggtacta
      481 cctgaaaaaa ccctttatcg ccgcctcggt tttcaacacc caaccgatgg tttccatcgc
      541 caacgcaagc ccaacccccg acgaccttta cgagcgcctg caggccatgg ggatcacgca
      601 tatcctcgtg aacgccgcag aaacccttta tctccacaaa aactggcctg agaaattctc
      661 cgccgccggc cgggagacgt tcgacgcctt ctggcaaaaa cacgcgctcc ccgttttcat
      721 cgatgcgggg cccggaccgt cggatttccg ccgcgtcgcc gtcttcgaac tcgtgcccga
      781 cagagaggcc ctccagcccc tccggccgcg cattcccaac cccctcctgg aaatcctgga
      841 cggcggaaat ccgggcatag aactcccttg acatcgcccg aatttgtggt aaactattct
      901 tgagcacggc gttgtgctcg tttcatcggc gcggtcgtac cgcgggacaa tgaagtcctc
      961 ctcgaaaacg gggaggattt tttatttccc atgcagccgt ctcggagacg atggggtctc
     1021 gtttcgaaag aaacgaggcc ttttttattc atcctatagg aggcgtacca tgttttccaa
     1081 aaaaaacaaa atcggacgct gggctttgcc ggtttatatc gggacagccc ttctgttcag
     1141 ccttaccttg acgccggaac agcttggggc cgaatcggcc gcgacgccgg ccgtggcctc
     1201 gatgtcgctt gaaaacacaa ccccacccat cacggcaaac ctccccgccc tcgataccgg
     1261 cgacaccgcc tgggtcctcg tgagcgcggc cctcgtgctc ctcatgacgc cgggcctcgc
     1321 cttcttctac ggcggacttg tccgcaagaa aaacgtcctc tcggtgttga tgcaatgttt
     1381 catgttgatg tgcctgatca gcatccagtg ggtcatgctc ggatactccc tctccttcgg
     1441 accggacatc aaaggactca tcggaaacct ggattggttc ggcctaaaaa acgtcgggct
     1501 ggcgcctttt ccggattact ccgccaccgt cccccatcaa gcgttcatga tctaccaggc
     1561 gatgttcgcc atcatcacgc ccgggctcat cttgggggcc ttcgccgaac gcatgaaatt
     1621 ctccgcgttc tgtctcttct ccctgctttg gaccacactg gtctacgatc ccgtggccca
     1681 ctgggtgtgg ggcatcggcg gtttcctgcg aaccggcggc gcgctggatt tcgccggagg
     1741 caccgtggtc cacgtcaacg ccggggtggc ggccctggcg gcggcgctgg tgctggggaa
     1801 acgccaggga tatcccgaca ccatgtcgcc gccccacaac ctgcctttcg ccgtcttggg
     1861 ggcggggctt ctttggttcg gttggttcgg ct
//
LOCUS       DAOTFD010000334         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_54548_length_1883_cov_4.351204, whole genome shotgun sequence.
ACCESSION   DAOTFD010000334 DAOTFD010000000
VERSION     DAOTFD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54548_length_1883_cov_4.351204"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="P5079_11845"
     CDS             <1..617
                     /locus_tag="P5079_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY30714.1"
                     /translation="EKVEADAVVCAVPLPDFLQILPPGWKKDYQGLVGAAHSAIVSVN
                     LWFRRPVMDELFVGLLDTPTQWLFNRYRLWQGRGVSPGYVSAVISAADGLSGKTGEEL
                     VSLVLDDLERCAPSLRREDLVHGSAVWEKHATLSPSPSNWGHRPGMRTPWRNFFLAGD
                     WVDCGLPPTMEAAARSGHQAADAVKEFLGQRHARPPAGSVPVSA"
     gene            634..1314
                     /locus_tag="P5079_11850"
     CDS             634..1314
                     /locus_tag="P5079_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRY30715.1"
                     /translation="MKAILFDCDGVIADSEGLHLSLFQQLLEELGVRLTKEQYYASYL
                     AMDDKACFRTALKDNGKPVSEEQIQELVGRKTALYKQAASRNLVIFPGVVEFVMAAAQ
                     KYPLAVASGALKEEVLLMVDTAGLRQYFDVFVAAEDVTRGKPAPDAYLKAMELLNRAC
                     PGKDIRPADCLVVEDSKHGIASAHAAGMKCLAVCTSYPVEALSAADKIVPYLTAAKLS
                     DLEALFRA"
     gene            1318..>1883
                     /locus_tag="P5079_11855"
     CDS             1318..>1883
                     /locus_tag="P5079_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRY30716.1"
                     /translation="MDAASPRQRFADIVKRGERRLDLAEAALVIAQEEYPLLEMEPYL
                     SRLDEMGKRVRGQLSHRGDPLAGLAALNKVLFKEEKFRGNEEEYYDPRNSFLNEVLDR
                     KTGIPITLSLLAMEVGRRAGLPLAGVGLPGHFIVTYLSPSRSFWIDPFDQGRLLSERD
                     CEQMVKRIYDGRVPFRRELMRPVSVFAIL"
BASE COUNT          311 a          576 c          636 g          360 t
ORIGIN      
        1 gggaaaaggt ggaggccgac gccgtcgttt gcgcggtgcc gttgccggat tttttgcaga
       61 tacttccgcc ggggtggaag aaggattatc aggggttggt cggcgcggcc cattcggcca
      121 tcgtgtccgt gaatctctgg ttccgccgac cggtcatgga cgaactcttc gtcggccttc
      181 tggacacgcc cacgcagtgg ctgttcaacc gataccgttt gtggcagggg cgtggcgttt
      241 cgccgggcta tgtctccgcg gtcatcagcg ccgccgacgg gttgtccggg aagacggggg
      301 aggagctggt ctctttggtg ttggacgatc tggaacgctg cgccccgtcg ctgaggcggg
      361 aggacctggt gcacggctcg gcggtgtggg aaaaacacgc cacgctgtcc ccgtcgccct
      421 ccaactgggg gcatcggccg gggatgcgca cgccctggcg aaacttcttt ttggccggcg
      481 actgggtgga ttgcggcctt ccgcccacca tggaggccgc cgcgcgcagc gggcaccaag
      541 cggccgacgc cgtgaaggaa tttttgggcc agcggcacgc ccggccgccg gccggatccg
      601 tgcccgtttc ggcgtgaggg gaggagacgt ttgttgaagg cgatactttt cgattgcgac
      661 ggcgtgatcg ccgattccga aggactgcac ctgtccctgt tccagcagtt gctggaggaa
      721 ctcggcgtcc gtttgaccaa agagcagtat tacgcctcct atttggccat ggacgacaag
      781 gcctgttttc ggacggcgtt gaaggacaac ggcaagccgg tgtccgaaga gcaaattcag
      841 gaactggtgg gacgcaagac cgccctctac aaacaggcgg cctcgcggaa cctggtgatt
      901 tttcccggcg tggtggagtt cgtgatggcg gcggcgcaaa aatatccgtt ggcggtcgcc
      961 tcgggcgcgt tgaaagagga ggtcctgctc atggtggaca ccgccggcct gcggcagtac
     1021 ttcgacgtct tcgtggccgc ggaggacgtg acccgcggca aaccggcgcc cgacgcctac
     1081 ctgaaggcga tggaactgtt gaacagggcc tgtcccggga aggatattcg tccggcggac
     1141 tgtctggtcg tcgaggattc caaacacggc atcgcctccg cgcacgccgc ggggatgaaa
     1201 tgcctggcgg tctgcacctc ctaccccgtc gaggcgttgt ccgccgccga caagatcgtg
     1261 ccgtacctca ccgcggccaa actttccgac ctggaagccc tttttcgggc ctagcggatg
     1321 gacgccgctt cgccgcgtca gcggttcgcc gacatcgtca aacgggggga acggcgtctg
     1381 gacctcgccg aggccgccct ggtcatcgcc caggaggagt atccgctgtt ggagatggag
     1441 ccctacctct cccgtctgga cgagatgggc aaacgcgtgc gcgggcagct gtcgcatcgc
     1501 ggagacccgt tggccggcct ggccgccctc aacaaggtcc ttttcaagga ggagaagttc
     1561 cgcggcaacg aggaggaata ctacgacccg cgcaacagtt tcctcaacga ggtgctcgac
     1621 cggaaaaccg ggatccccat cacgctgtct ctcctcgcca tggaagtggg ccgccgcgcc
     1681 ggtctgccgc tggcgggcgt gggactgccc ggccatttca tcgtgaccta tctttctccc
     1741 agccggtctt tttggatcga tcccttcgac cagggccgcc tgttgagcga acgggactgc
     1801 gaacagatgg tgaaacggat ctacgacggt cgggtgccgt tccgccggga actgatgcgg
     1861 cccgtctcgg tgttcgccat cct
//
LOCUS       DAOTFD010000335         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_55013_length_1874_cov_3.291369, whole genome shotgun sequence.
ACCESSION   DAOTFD010000335 DAOTFD010000000
VERSION     DAOTFD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55013_length_1874_cov_3.291369"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="P5079_11860"
     CDS             <1..350
                     /locus_tag="P5079_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY30717.1"
                     /translation="IVFGTPVYWYGPTGLMKLFIDRLVYFNCPPNRKKIKGKPAVLVM
                     PLEETNPETAAGTVDFFEKCLSYLEMEFFGKVVAGGVGRKGDALTKIGPLDAARKLGG
                     RLAERLAHGRRPK"
     gene            complement(416..1411)
                     /locus_tag="P5079_11865"
     CDS             complement(416..1411)
                     /locus_tag="P5079_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRY30718.1"
                     /translation="MELTTTYLGLPLKNPVVGSASSLWDKLDNIRSAEEAGLSAVVLH
                     SLFEEQISHERYELQHRLTEHTESHAESLTYFPEAGDFVLGPEEYLEHIRKAKQAVKI
                     PIIASLNGHSLGGWTEYAKLMQQAGADALELNIYFLATDTALTGQEVEKTYLDILKAV
                     KSCVSIPVAVKLGPWFSAMGNMAKSLADAGADGLVLFNRFYQPDIDLENLEVVPNVVL
                     STPLSMRLPLRWIAILRGNVKAYLAATGGIHQAGDALKVLLAGADVTMLCSTLFRNGI
                     PHAKTILSDMKMWMEMHEYDSVRQLCGSMSHKAVDNPAAFERANYMKALQGFGLK"
     gene            complement(1411..>1874)
                     /locus_tag="P5079_11870"
     CDS             complement(1411..>1874)
                     /locus_tag="P5079_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRY30719.1"
                     /translation="TYRNVYVAQVSMGARDEHTIRAFLEAEAYRGPSLIVAYSHCIAH
                     GINMVTAMRDQKEAVQSGHWPLFRYNPDLAAQGKNPLQLDSPAPTIKLEEFAYKQNRF
                     KMLTKSQPDEAKRLIQVAQQDADIRRRFYEYLASQPGASAPPAGAPAKEGV"
BASE COUNT          356 a          550 c          590 g          378 t
ORIGIN      
        1 ccatcgtttt cggcacgccg gtctactggt acggtcccac cggattgatg aaactcttta
       61 tcgaccggct ggtgtacttt aattgccccc caaaccggaa gaaaataaaa gggaagccgg
      121 cggtgctggt gatgccgctg gaagagacaa accccgagac cgccgccggg acggtcgatt
      181 tctttgagaa atgtctctct tatcttgaaa tggaattctt cgggaaggtg gttgccggcg
      241 gggtcggccg aaaaggcgac gcgctgacga aaatcggccc gctggacgcg gcgcggaagc
      301 tgggcggccg gctcgctgaa cggctcgcgc acggccgccg tccaaaatga ctttttaacg
      361 gccccggcgc cgcgatcgac gaaaaagtcg tcgagaaaaa ggcgggccga cgcccttatt
      421 taagaccgaa cccctgcaag gccttcatgt aattggccct ctcaaaagcc gccgggttgt
      481 cgacggcttt atggctcata ctcccgcaaa gttgtcgcac cgaatcgtat tcgtgcatct
      541 ccatccacat cttcatgtcg ctgaggatgg ttttggcgtg ggggatgccg ttccggaaaa
      601 gcgtggaaca caacatggtc acgtcggccc ccgccagaag gaccttgagg gcgtcgccgg
      661 cctggtggat cccgccggtg gcggccagat aggccttcac gttcccgcgc aggatggcga
      721 tccaacgcaa cggcaggcgc atggagagcg gcgtgctcaa aaccacgttc gggacgacct
      781 ccaggttctc caggtcgatg tccggctgat agaaccgatt gaacaacacc aacccatcgg
      841 cgccggcgtc ggcgagcgat ttggccatgt tccccatggc gctgaaccac ggccccaatt
      901 tcaccgccac cggaatggaa acgcaggact tcaccgcttt caggatgtcc agataggttt
      961 tttccacctc ctggccggtg agggcggtat cggtggccag gaaatagatg ttcagctcca
     1021 acgcgtcggc gccggcctgc tgcatgagct tggcgtattc cgtccagccg ccgagcgagt
     1081 ggccgttcaa actggcgata atggggatct tcaccgcctg cttggccttg cggatgtgtt
     1141 ccaggtattc ctccgggccc aagacgaaat cgccggcctc ggggaaatat gtgagcgatt
     1201 cggcgtgact ctcggtgtgt tcggtcagac gatgttgaag ttcgtagcgt tcatgcgata
     1261 tctgctcttc gaagagcgaa tgaagcacca cggccgagag gccggcctcc tcggccgacc
     1321 ggatgttgtc caacttgtcc cagagcgaag aagccgagcc gacgacggga tttttcagcg
     1381 gaagtccgag atacgtggtc gtgagttcca tcagaccccc tcttttgccg gcgcgccggc
     1441 gggcggagcg gaggcccccg gctgcgaagc gaggtattca tagaaccgtc gccggatgtc
     1501 cgcgtcctgt tgggcgacct gtatgagtcg tttcgcctcg tcgggctggc tcttggtgag
     1561 catcttgaaa cggttctgtt tgtaggcgaa ctcctccagt tttatcgtcg gcgcggggga
     1621 gtccaactgc aacgggttct tcccctgcgc ggccaggtcc gggttgtaac ggaacagcgg
     1681 ccaatgcccc gattggacgg cctctttctg gtcccgcatg gcggtgacca tgttgatgcc
     1741 gtgcgcgatg cagtgagagt aggccacgat gagggacggc ccccgatagg cctcggcctc
     1801 gaggaacgcc cggatggtgt gttcgtcccg ggcgcccatg gacacctggg ccacgtacac
     1861 gttgcggtag gtca
//
LOCUS       DAOTFD010000336         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_55641_length_1862_cov_4.175982, whole genome shotgun sequence.
ACCESSION   DAOTFD010000336 DAOTFD010000000
VERSION     DAOTFD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55641_length_1862_cov_4.175982"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="P5079_11875"
     CDS             <1..457
                     /locus_tag="P5079_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30720.1"
                     /translation="YGSPDSDGIGAEGGAGAEAPNSTSGGSLARVSDGRDTQNNFADF
                     QSDPSPTPREPNDPSGLVRTDYSGTIRYPNPFFPRVHGTVTLSTPERIMGPLRDIRIY
                     DETGELVRLLQAPQWDGRNDDGEFVASGLYFYVVQTELGKSRGKVTLIR"
     gene            496..1521
                     /locus_tag="P5079_11880"
     CDS             496..1521
                     /locus_tag="P5079_11880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30721.1"
                     /translation="MKRKPKRIGRRSFFYFSPSHRFFIFLFTISLFHCSAAPLWAGKR
                     ILKQGQTAAAFLKVPVSARAVGVGEAFVGFGDDVSVLQYNPAGIAALPSFHMTMSHAL
                     YFQETALSSLEAAVPWGRSALGVTYRQFVADDDARDVTGRRDGDIEHKDMAVGLAYSR
                     RFGRRGAVGVAVKNAARKIDRSGSATVRTDFSVEQSVWMGDAGLFLALPSRGTLGFAV
                     QNIGSGAAFEYSGDVAGSPFGPKTTGAREALPLVVRLGGLHPVGRFGFAWEADLPRDN
                     RVIAAVGAEYPVTKFFSLRGGFRYQRYIDFSLGLGVSKSFFSVDYAFSPRVDLGTLHR
                     ATVGIRF"
     gene            1527..1727
                     /locus_tag="P5079_11885"
     CDS             1527..1727
                     /locus_tag="P5079_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30722.1"
                     /translation="MDQLTKIFLLMLFGSILAGAIIVAVKPDYRVDVILRSNSQYFAQ
                     VEIAGNTVEDPAKPAEENVPED"
BASE COUNT          318 a          540 c          613 g          391 t
ORIGIN      
        1 atacggttcc cccgattcgg acggcatcgg cgccgagggg ggggcgggcg cggaggcgcc
       61 caacagcacc tccggaggct cgttggcccg cgtgtccgac ggaagagaca cgcagaacaa
      121 tttcgcggat ttccagtcgg atcccagccc gaccccgcgc gaaccgaacg acccgtccgg
      181 gctggtgaga acggattaca gcggcaccat ccgctacccc aacccgtttt tcccgcgggt
      241 ccacggcacc gtcacgctga gcacgccgga acggatcatg ggcccgttgc gggacatccg
      301 catctacgac gagaccggcg agctggtgcg cctccttcag gcgccccaat gggacggacg
      361 gaacgacgac ggcgaattcg tggcttccgg gttgtatttc tacgtggtgc agacggagct
      421 cgggaaatcg cgcgggaagg taactttaat acggtgaatg gtgaagtggt gaatagcgaa
      481 atggaaaaga cggaaatgaa gagaaagccg aagaggatcg gccgaaggtc gtttttttat
      541 ttttcaccat cccaccgttt ttttattttt ctgttcacca tttcactgtt ccactgttcc
      601 gccgccccgc tctgggccgg caagcggatc ctcaaacagg ggcagacggc ggccgcgttc
      661 ttgaaggtgc cggtgtcggc ccgggcggtg ggggtgggag aggccttcgt gggtttcggc
      721 gacgacgtgt cggtgctgca atacaacccg gcggggatcg ccgccctccc gtctttccat
      781 atgacgatgt ctcatgcgct gtattttcaa gagaccgctc tgtcgtctct ggaggccgcg
      841 gtgccgtggg gccgttcggc gttgggggtg acctaccgcc agttcgtggc cgacgacgac
      901 gcgcgggacg tcacgggccg aagggacgga gacatcgagc ataaggacat ggcggtgggg
      961 ctcgcgtatt cccggcggtt cggccggcgc ggggcggtcg gggtcgccgt gaagaacgcg
     1021 gcgcggaaga tcgaccgctc cggcagcgcg acggtccgaa ccgatttttc cgtggaacag
     1081 agcgtgtgga tgggggacgc gggattgttt ttggctctgc cttcccgggg gaccctcggg
     1141 tttgccgtcc agaacatcgg atcgggggcg gctttcgaat acagcggcga cgttgcgggg
     1201 agccctttcg gcccgaagac gacgggggcg cgggaggcgc ttccgttggt ggtgcgtttg
     1261 ggcggacttc atcccgtggg gcgcttcggt ttcgcctggg aggccgatct gccgcgcgat
     1321 aatcgcgtca ttgccgccgt gggggcggaa tatccggtga cgaaattctt ttcgttgcga
     1381 ggcggcttcc gctatcagcg gtacatcgat ttttcgctcg gcctcggcgt ttcaaaaagt
     1441 tttttcagcg tggattacgc tttttctccg cgggtggatc tgggcactct ccaccgcgcc
     1501 accgtcggga tccgcttcta ggcaaaatgg accaactgac caaaatcttc ctcctgatgc
     1561 ttttcggctc catcctggcc ggcgccatca tcgtggccgt caaaccggat taccgcgtcg
     1621 acgtcatcct gcgttccaat tcccagtatt ttgcccaggt cgagatcgcc ggaaatacgg
     1681 tcgaagatcc tgcgaaaccg gcggaagaga acgttccgga agactaacat ggataaagcg
     1741 gcccgaacga gacttatcat ggccttttgc tatgcgctga tcgtgggtgt ggcgttttcc
     1801 atgggttatt tctccgaggt tcccgcgtac cggcctttgg cctccctgtt gcccttcctg
     1861 cc
//
LOCUS       DAOTFD010000337         1831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_57223_length_1831_cov_4.057995, whole genome shotgun sequence.
ACCESSION   DAOTFD010000337 DAOTFD010000000
VERSION     DAOTFD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57223_length_1831_cov_4.057995"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="rfbA"
                     /locus_tag="P5079_11890"
     CDS             complement(<1..677)
                     /gene="rfbA"
                     /locus_tag="P5079_11890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRY30723.1"
                     /translation="MKGIILAGGSGTRLYPLTRGISKQLVPIYNKPMIYYPLSVLMLA
                     GIRDVLIITTPHEQDGFKRLLGDGRWLGLNFQYAAQPSPGGLAQAFLIGRTFVGSDRV
                     CLILGDNIFYGHGLPTYLKKAVDREKGATVFAYQVRDPQQYGVVEFDGSGKAVSLEEK
                     PAKPRSSHAVTGLYFYDNRVLDIAAELKPSPRGELEITDVNKAYLRQGELHVEKLGRG
                     IAWLDTGT"
     gene            complement(709..>1831)
                     /locus_tag="P5079_11895"
     CDS             complement(709..>1831)
                     /locus_tag="P5079_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRY30724.1"
                     /translation="RGQYADIVHFLLDKDGLDLASRPKGLLPLHQYKKEVRTPFEEHL
                     LESLELTRTEGDVCRAHFTVADDYLEEFQWFARKCVPTAEKKHKVKFDLSFSVQKRST
                     HTLAVDMENRPFRRADGRLLLRPGGHGALIENLNDLNGDIVFIKNIDNAAPARLQGEV
                     VRWKKALGGLLIELQEEIFRLMTWLESRPEDDGVIDQAFRLLLGRLSITVPPSVRQGS
                     RMEHRRFLMSKLNRPLRVCGAVENTGEPGGAPFWVQAKSGEQSIQIVEQHQVDPNSSD
                     QQAIFAEATHFNPVDIVCALRDRTGRPFDLRRHVDPSAVFIAKKSEAGRELKALERPG
                     LWNGGMADWNTVFVEVPAGTFNPFKTLRDLLRDGHRAEI"
BASE COUNT          318 a          558 c          598 g          357 t
ORIGIN      
        1 gtgccggtgt cgagccaggc gatgccgcgg cccagtttct ccacgtgcag ttccccctgc
       61 cgcaggtagg ccttgttgac gtcggtgatc tccagttcgc ctcgaggcga aggtttcagt
      121 tcggcggcga tgtccaacac gcggttgtcg taaaagtaaa gtccggtcac ggcgtgggat
      181 gaacgcggtt tggccggttt ctcctccagg ctgacggcct tgccggaacc gtcgaactcc
      241 accacgccgt attgctgcgg atcgcgcacc tggtaggcga acaccgtggc ccctttttcc
      301 cggtccaccg cttttttcaa ataggtgggc aggccgtgtc cgtaaaagat attgtcgccc
      361 agaatgaggc agacgcggtc cgagccgacg aaggtccgac cgatgaggaa cgcctgcgcc
      421 aacccgccgg gcgacggctg ggcggcgtat tgaaaattga ggccgagcca gcggccgtcc
      481 cccaaaaggc gtttgaatcc gtcttgttcg tgcggcgtgg tgatgatgag cacgtcgcgg
      541 atgcccgcca acatcagcac cgacagcgga taatagatca tgggcttgtt gtagatgggg
      601 accagctgct tgctgatgcc gcgcgtgagg ggatagagcc gcgtgccgga accgccggcc
      661 aagatgattc ctttcatgtg aggattctcc tttttatcgt ctacgaaatc aaatctccgc
      721 ccggtgtccg tcccgcagaa ggtcgcggag cgtcttgaac gggttgaacg tccccgcggg
      781 gacctccacg aaaacggtgt tccaatcggc catgccgccg ttccagagcc cgggtcgttc
      841 caacgccttc agttcccgtc cggcctcgga ttttttggcg atgaacaccg cggaggggtc
      901 cacgtggcgg cgcaaatcga acggccgccc ggtgcggtcg cgaagggcgc agacgatgtc
      961 caccgggttg aaatgggtgg cctcggcgaa gatggcctgc tggtcggaag agttcgggtc
     1021 cacctggtgc tgttcgacga tctggatgga ctgctccccg cttttggcct gcacccagaa
     1081 cggcgcgccg ccgggctcgc cggtgttctc caccgcgccg cagacgcgca acggccggtt
     1141 cagcttggac atcaggaacc gccggtgttc catacgggac ccctgccgca cgctgggcgg
     1201 cacggtgatg gaaaggcgcc ccaacagcag acggaacgcc tggtcgatga cgccgtcgtc
     1261 ctcgggccga gactccagcc aggtcatgag ccggaatatc tcctcctgca gctcgatgag
     1321 aaggccgccg agggccttct tccagcggac cacttccccc tgaagccgcg ccggcgccgc
     1381 gttgtcgatg ttcttgatga acacgatatc gccgttgagg tcgttcaggt tctcgatgag
     1441 cgcgccgtgg ccgccgggcc ggagcagcag ccggccgtcg gcgcggcgga acggccggtt
     1501 ctccatgtcc accgccaacg tatgcgtgga gcgtttttgg acggaaaagg acaggtcgaa
     1561 cttgaccttg tgtttctttt cagcggtcgg aacgcacttg cgggcgaacc actggaactc
     1621 ctccaggtaa tcatcggcca cggtgaagtg cgcccggcag acgtcgccct ccgtgcgggt
     1681 gagttccagg gattccagga gatgctcttc gaacggggtg cggacctctt ttttgtattg
     1741 atggaggggc aacaaaccct tggggcggga ggccaggtcc aggccgtcct tgtccagcag
     1801 gaaatggacg atgtccgcgt attggccgcg t
//
LOCUS       DAOTFD010000338         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_57925_length_1818_cov_2.988088, whole genome shotgun sequence.
ACCESSION   DAOTFD010000338 DAOTFD010000000
VERSION     DAOTFD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57925_length_1818_cov_2.988088"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="P5079_11900"
     CDS             <1..1565
                     /locus_tag="P5079_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30725.1"
                     /translation="PFYTRSLRDKRWTLRVEPGQNSREGLFAKSIFGYKLTDNTYTRL
                     YWDHFEKTGNGVGAEYNYYLPDVKGSVYSYRIEDRVAQTERWNFRAGHWQQLKPRWAL
                     QANAVFQSDQDFNNLYFRDDYERVRQRADSDAALTYQNPLYSARVSFEQDMTFNTTHQ
                     AFVRDHTVLPQLNLQSSPLNLGKREVYLTLTGNFINTYQRPEVTDLLTQKPTFYHERD
                     LFKQSADGTLDLSKKIQLGKNTTLKPAVGVIEEWQAWRESGVSEIDRKDLYQGRVYTK
                     TNLRRRLTRDLDGDLTHTYRVRWSPNQMKRDHSSSLLDSGTEANSLDLFLSYRMGRLL
                     WVRASSGYDLRVLQGETIETVRQKISPPNVEVNITPTQTLSFFYRQSVLLYPARKTQT
                     SQFSVQVGEIEKPHLESGLSYNVGTPHQLQVRHGAAFPLTKGWWVNGGVQYNAIGTYR
                     MDYDSVKFIEKYLILKRDLHCWKAKAEIRQRPGVEEVFFTIDLKTNFAARQDMASPEE
                     EQFYPAREHESP"
     gene            1750..>1818
                     /locus_tag="P5079_11905"
     CDS             1750..>1818
                     /locus_tag="P5079_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY30726.1"
                     /translation="MKLLVTGGAGFIGSHFVHYWLDK"
BASE COUNT          419 a          597 c          454 g          348 t
ORIGIN      
        1 cgcccttcta cacccgctct ctccgagaca aacgctggac cttgcgggtg gaacccggac
       61 agaactcccg cgaaggcctt tttgcgaaat ccatcttcgg ctataaactg acggacaaca
      121 cctacacgcg tctctactgg gaccatttcg aaaaaacagg caacggggtc ggcgctgaat
      181 acaactatta cctgcccgac gtcaaaggtt ccgtctattc ctatcgcatc gaagaccgcg
      241 tggcccaaac ggagcgctgg aacttccgcg ccggccattg gcaacagctg aaaccccggt
      301 gggcccttca agccaacgcc gtcttccaaa gcgaccagga cttcaataat ttgtatttcc
      361 gcgacgatta cgagcgtgtc cgtcagcgag ccgactcaga cgccgccctc acctaccaga
      421 acccgctcta ctcggcacgg gtctccttcg agcaggatat gacctttaat accacccacc
      481 aggcgttcgt ccgcgaccac acggtgctgc cgcaattgaa tcttcagtcc tctccgttga
      541 acctggggaa acgggaggtg tacctcacgc tgaccggaaa tttcatcaat acgtatcaac
      601 gcccggaggt caccgaccta ctgacccaaa aaccgacctt ctatcacgag cgcgacctct
      661 tcaagcaaag cgccgacggc accctggacc tgtcgaaaaa aatacaactg ggaaaaaaca
      721 ccaccctcaa gcccgccgtc ggcgtgatcg aagaatggca ggcgtggcgg gaatccggcg
      781 tcagcgaaat cgaccggaaa gatctttatc agggccgcgt ctataccaag accaaccttc
      841 gccgccgcct cacccgcgac ctggacggcg atttgaccca tacctaccgc gtccgttggt
      901 cgccgaacca gatgaaacgc gaccattctt cctccctcct cgacagcggc acggaagcga
      961 actccctgga cctgttcctt tcttatcgca tgggccgcct cctgtgggtg cgcgccagca
     1021 gcggctacga cctgcgcgtc ctccaagggg aaaccatcga aacggtgcgc cagaagattt
     1081 ctcccccgaa cgtggaagta aacatcacgc ccacccaaac cctttcgttc ttctatcgcc
     1141 aaagcgttct tctttatccc gcacggaaaa cccagacaag ccagttctcc gtccaggtgg
     1201 gggaaatcga aaagccgcac ctcgagtccg gcctcagcta caacgtcggc accccgcacc
     1261 aactgcaggt ccggcacggc gccgccttcc ccctgaccaa aggctggtgg gtgaacggcg
     1321 gcgtgcagta caacgccatc gggacctatc ggatggatta tgattccgtg aaattcatcg
     1381 agaaatacct catcctcaag cgcgacctgc actgctggaa agccaaggcc gagatacggc
     1441 aacggcccgg cgtcgaagag gtatttttca ccattgacct gaagaccaac ttcgccgccc
     1501 gtcaagacat ggcctccccg gaagaggaac agttttatcc ggcccgggag catgaatcgc
     1561 cctgacggag gacaaagcca ctccggccgc tggctttgcc ggtccgttca tttgggcgaa
     1621 cgttttgagc atctggttcg agcttgacga tgccggtcat tttgcataca atcctttgcc
     1681 ccgacggcaa tcgttaccgg atggccgcgg ggttattgaa tcgttccatc ggaggacgga
     1741 cttaacatca tgaaactcct tgtgaccggc ggcgccgggt tcatcggctc ccatttcgtc
     1801 cattattggc tcgacaaa
//
LOCUS       DAOTFD010000339         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_58065_length_1815_cov_4.131818, whole genome shotgun sequence.
ACCESSION   DAOTFD010000339 DAOTFD010000000
VERSION     DAOTFD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58065_length_1815_cov_4.131818"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="P5079_11910"
     CDS             <1..355
                     /locus_tag="P5079_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30727.1"
                     /translation="VSRLAELYRSQAARREKVFSGPLSENSPDVMVMVKPGYRVSWQT
                     ALGGAPAALMENHMSKWSGDHSCNDPADIPGILIANRRINESHPRLSQVAPTILKTLG
                     ADRAPHMDKELTLSP"
     gene            complement(352..732)
                     /locus_tag="P5079_11915"
     CDS             complement(352..732)
                     /locus_tag="P5079_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY30728.1"
                     /translation="MGDGKKRILVVDDEPFLLDLMLERCDEIGVTAIPAENGAEGLQK
                     AASEKPDAIIVDNRMPGMSGMEVIAKLKSDPATQDIPVMLLSADSKQLHEAEAKKLGA
                     YGVLMKPVKMADLKAILEGCLGGF"
     gene            complement(855..>1815)
                     /locus_tag="P5079_11920"
     CDS             complement(855..>1815)
                     /locus_tag="P5079_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30729.1"
                     /translation="FDIPKFVDLFGLLCGTPVLVYQGLFLLLFCSGIFYLLRDDFLIG
                     VLVVAIGGAFFFCEMISKEENAVLVSARFGIALIPIAFLLVATGMNGLFEMFRIYSGG
                     RTGPTLAVAVFMGGLFFMSPLWRIYRSPNNFTNHSAYEESYAPTDWSVGGGRNIVSLP
                     PLKKSDVPPFYFRSDELVAADGIIEYPMFIGDYFNPYYYYQVFHKKRVAVGYVPELRF
                     SPLSSRNESVFGDTPIDYVLSRLPAGRRRRRFENMVPLTDAGYLKRKYTRWLLIVHKD
                     PQKEFGAAAFPLFRPLPVVGEILLRAYGPPSYQDEHIVVWRIG"
BASE COUNT          426 a          590 c          474 g          325 t
ORIGIN      
        1 ggtgagccgc ctggcggaac tctaccgcag ccaggcggcc cggcgggaga aggttttttc
       61 aggcccgctg tccgaaaaca gcccggacgt catggtgatg gtcaaaccgg ggtaccgggt
      121 ctcctggcaa acggccctgg gcggcgcgcc ggcggcgctg atggaaaacc atatgagcaa
      181 atggagcgga gaccactcgt gcaacgaccc ggcggacatc cccggcatcc tcatcgccaa
      241 ccgccggata aacgaaagcc acccgcgtct gtctcaggtg gctcccacga tcttgaaaac
      301 cctcggcgcc gaccgcgcgc cgcacatgga caaggaactg acgctctccc cctaaaatcc
      361 gccgaggcat ccctccagga tcgccttcag gtccgccatt ttcaccggtt tcatcagcac
      421 gccgtacgcc cccaactttt tggcctccgc ctcgtgcagt tgcttggagt ccgcggaaag
      481 aagcatcacc gggatgtcct gggtggcggg gtcggacttc agcttggcga tgacctccat
      541 gcccgacatc cccggcatgc ggttatccac gatgatggcg tcgggttttt ccgacgccgc
      601 cttctgcaac ccctcggcgc cgttttccgc cgggatggcg gtcacgccga tctcgtcgca
      661 ccgttccagc atcagatcca acaggaacgg ctcatcgtcc accaccaaaa tcctcttttt
      721 cccatcaccc ataaaccccc tccttcttct tttttccacc cgaccggaaa gattttagca
      781 cttttggccg gtcgaaagcg gggcccgaaa ccggcgccgc acggtcccga tgagaatccc
      841 ccgtccccga gggctcatcc gattcgccac accacgatgt gttcgtcctg ataggagggg
      901 gggccgtacg cccgcaacag gatttccccc accaccggca ggggacggaa caaagggaac
      961 gccgcggccc cgaactcctt ttgggggtct ttatggacga tcaacaacca gcgcgtgtat
     1021 ttccttttaa ggtatcccgc atcggtcaag gggaccatgt tttcaaaacg gcgccgccgg
     1081 cgcccggcgg gcaatcgact caaaacgtaa tcgatggggg tatctccgaa aacactttca
     1141 ttccgggacg agagcgggga aaaacgcaat tccgggacat agccgaccgc cacccgtttt
     1201 ttgtgaaata cctggtaata ataatacgga ttaaaataat ccccgataaa catcggatat
     1261 tcgatgatgc cgtcggcggc gacgagttca tctgacctga aataaaatgg cgggacgtcg
     1321 gattttttca gcggcggcaa cgaaacgatg ttccgtccgc ctcccaccga ccaatcggtc
     1381 ggcgcatagg actcctcgta ggcggagtga ttggtgaaat tgttggggga ccgataaatc
     1441 cgccacagcg ggctcataaa aaacaatcct cccataaaaa ccgccacggc gagggtcggc
     1501 ccggtcctcc cgcccgaata aatccggaac atttcgaaca agccgttcat ccccgtcgcc
     1561 acgaggagga aagcgatcgg gatcagcgca atcccgaacc gcgcgctcac caacaccgcg
     1621 ttttcctcct tcgagatcat ttcacagaaa aaaaacgccc ccccgatggc cacgaccagc
     1681 acgcctatca aaaaatcgtc cctcagcagg taaaaaatgc ccgaacaaaa caacaacaga
     1741 aaaagcccct gatacacaag caccggcgtg ccgcacagca aaccgaacag gtcgacaaac
     1801 ttggggatat cgaac
//
LOCUS       DAOTFD010000340         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_59093_length_1798_cov_3.377510, whole genome shotgun sequence.
ACCESSION   DAOTFD010000340 DAOTFD010000000
VERSION     DAOTFD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59093_length_1798_cov_3.377510"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..>1798
                     /locus_tag="P5079_11925"
     CDS             157..>1798
                     /locus_tag="P5079_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30730.1"
                     /translation="MPTKPKPVVLPPVWLAGVMLSVTTDAFGNETKSYSQQTFIIMAN
                     QAKLKTVTSWSVSTGVDGAVTTTDAYTMVYKYWGEELYAGGRLPSLKELKDYAQSLWG
                     KYQVRLGEMMSADQVGVIHSRTEDAFGNVTDAWSTQTFKIINNQAKLETVTSWSTALS
                     IDGTVTKTDAYVMKYTYREDTAENRQLGRVGHLVSAELLGTDMDGDGRFDGRIYSHTK
                     DAFGNVTESWSTQTFVIIANQAKLKEVTSASETKGVDGVVSVTDPYTIRYTYYGEELY
                     ANGRVPSRNEIKAWIARNDHRSIFGQLQSAVLLNTIHSRTVDLFGTISNSWSTQTFVI
                     VAGVAKLKEVTTWSKSESFDGSTTTTDPYTIRYNYFGEGLYAGGRMPYREEILAWMKV
                     NGRKFGQLQSAAMLLRYQPPYEGHTVVKNGKVTIVQQPGQKYGMHSVSVDAFGNVSET
                     WTTQNFIIVAGVAKVGTEYSYTLSKSLDMTTTYSYSLVTYKYHEDTWLNRQLHRVGQY
                     KSASGTVWSFSNSLNFDNTTTVTVSVTNNVYKIVAGKPV"
BASE COUNT          388 a          559 c          516 g          335 t
ORIGIN      
        1 ccgccgcccg aggtggtgcc ggttcccgtt ccggcgccga tcccggtccc gcggccggaa
       61 ccccgtccga gggggaaggt ggttcctcgc ccgacgccgg ggcccatcgt tgattttcct
      121 cgtcggccgc ctgtgtggcg gaagccgccc atccccgtgc cgacaaaacc caaaccggtg
      181 gtgctgccgc ccgtttggct ggcgggggtc atgctttccg tcaccacgga cgctttcggc
      241 aacgagacca agtcgtattc ccaacagacc ttcatcatca tggccaacca ggccaaattg
      301 aagaccgtca cttcctggtc ggtgtccacc ggtgtggacg gcgccgtgac caccaccgat
      361 gcctacacca tggtctacaa gtattggggc gaagagctct atgcgggagg gcggctgccc
      421 agcctgaaag aattgaagga ctacgcgcag tcgctctggg gcaagtatca ggtccgtctc
      481 ggggaaatga tgagcgccga tcaggtcggt gtgatccatt cgcgcaccga ggatgccttc
      541 ggcaacgtga cggatgcctg gtccacgcag acgttcaaga tcatcaataa ccaggccaag
      601 ctggagaccg tcacctcctg gtccacggcc ttgtccatag acggcacggt gacgaagacg
      661 gacgcctatg tgatgaaata cacctaccgc gaggacaccg cggagaaccg gcagctcggt
      721 cgcgtcggtc atctggtttc ggctgaactg ctggggacgg atatggacgg ggacggaaga
      781 ttcgacggcc ggatttactc ccacaccaag gatgccttcg gcaacgtgac agaaagctgg
      841 tccactcaaa ctttcgtcat catcgccaac caggccaaac tgaaggaggt gaccagcgcc
      901 tcggagacca agggggtgga cggggtcgtc tccgtcacgg atccctacac catccgctac
      961 acctactacg gcgaagaact ttatgccaac gggcgggtcc ccagccgtaa cgagataaag
     1021 gcctggattg ctcggaacga ccaccggtcg attttcggcc aactgcagtc ggcggttctt
     1081 ctgaacacca tccattcgcg gacggtggac ctgttcggca ccatttccaa cagctggtcc
     1141 actcagacgt tcgtcatcgt cgccggggtg gccaaactga aagaagtcac cacgtggtcc
     1201 aagtccgagt ctttcgacgg gtccaccacc accaccgacc cctacaccat tcgttacaac
     1261 tatttcggag agggcttgta cgccggcggg cgcatgccct accgcgagga gatactcgcc
     1321 tggatgaagg tgaacggccg caagttcgga caactccaga gcgccgccat gctgcttcgg
     1381 tatcagcccc cctatgaggg ccataccgtg gtcaaaaatg ggaaggtcac cattgttcag
     1441 cagcccggtc aaaaatacgg catgcacagc gtcagcgtcg atgcgttcgg caacgtgagc
     1501 gaaacctgga cgacgcagaa cttcatcatc gtggccggcg tggcgaaggt gggaacggaa
     1561 tactcctaca ccctttcgaa gtcgctggac atgaccacca cctattccta ttcgctggtc
     1621 acctataaat atcacgaaga cacctggttg aaccggcagt tgcaccgggt cggtcaatat
     1681 aagtccgcgt ccggcacggt gtggtctttc tccaattccc tcaatttcga caacaccacc
     1741 acggtgacgg tgtcggtcac caacaacgtc tataagatcg tcgcgggaaa gcccgtgg
//
LOCUS       DAOTFD010000341         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_59799_length_1786_cov_2.524552, whole genome shotgun sequence.
ACCESSION   DAOTFD010000341 DAOTFD010000000
VERSION     DAOTFD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59799_length_1786_cov_2.524552"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5079_11930"
     CDS             complement(<1..225)
                     /locus_tag="P5079_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRY30731.1"
                     /translation="MKKNVRPKSPFHRKTILITSGPTREYLDPVRFISNDSSGRMGRA
                     LALVPGHDLPLALHGRHQAINTSLALAALEI"
     gene            complement(233..778)
                     /locus_tag="P5079_11935"
     CDS             complement(233..778)
                     /locus_tag="P5079_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRY30732.1"
                     /translation="MEIVLCISGSIAAYKSAEIVRCFRRKKADVTCVLTPAARHFVTP
                     LTLSTLTGRPVPQDMFAEPLWNMAHLSLTEKADAILMAPASADLLAKLAHGLADDLPS
                     TLALAASQPVFIAPAMHEGMWLHPATQANVGLCKGYGYRFIGPARGEMASGVVGMGRL
                     EEPALIVDAVLSALKTSRKKR"
     gene            complement(810..1217)
                     /locus_tag="P5079_11940"
     CDS             complement(810..1217)
                     /locus_tag="P5079_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30733.1"
                     /translation="MSEQKKKEEKVSKENWSLTQLMLDVGQDKYRLIKTALRWAQEIK
                     KRDQSTEPVPVLINMALKEILAGGVSLEEIEKLPPVPKSDGKPLELNLKALEVEDEKE
                     KEKGKEKAKAKDDDEEDEDTEKKKKSKKKDKED"
     gene            complement(1229..>1786)
                     /gene="gmk"
                     /locus_tag="P5079_11945"
     CDS             complement(1229..>1786)
                     /gene="gmk"
                     /locus_tag="P5079_11945"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRY30734.1"
                     /translation="GKSTLCQALLKERADLVYSVSATTRPARVSEVNGKDYFFVTVDQ
                     FKKKIDEGEFLEWALVHDHYYGTPRSFIDDQLARGADILLDVDPQGALAIQDKYPQCV
                     SIFVYPPNWESLERRLRTRGQDDEETIHKRLSNARHELTYLQHYNYLVVNNDIREAMN
                     DFLAIIRAEHHRVCRYSEKFLCLTK"
BASE COUNT          304 a          511 c          558 g          413 t
ORIGIN      
        1 gatttcgaga gctgccaggg ccagactggt attgattgcc tgatggcgac cgtggagcgc
       61 caggggcagg tcgtggccgg gaacgagggc cagggcgcgg cccatgcggc cggaggagtc
      121 gttggagatg aaacggaccg ggtcgagata ttcgcgcgtg ggacccgacg tgatgaggat
      181 tgtctttcgg tggaacgggg acttcggacg gacgtttttc ttcacgccgg actcagcgtt
      241 ttttgcggga tgttttcaaa gccgacagga cagcgtccac gatgagggcc ggttcttcca
      301 gtcgtcccat ccccacgaca ccggaagcca tctcgccccg ggcggggccg atgaaacggt
      361 atccataccc cttgcagagg ccgacgttgg cctgggtcgc cgggtggagc cacatgcctt
      421 cgtgcatggc cggcgcgatg aagaccggct gggaagccgc caacgccagg gtgctcggca
      481 gatcgtccgc caggccgtgg gccaatttgg ccaggaggtc cgccgacgcc ggcgccatca
      541 ggatggcgtc cgccttctcg gtcagagaga gatgggccat gttccaaaga ggttcggcga
      601 acatgtcctg cgggacgggg cggccggtga gcgtcgagag ggtcaagggc gtgacgaaat
      661 gacgggcggc cggcgtgagg acgcaggtga cgtccgcctt tttccggcgg aaacaccgga
      721 cgatctccgc ggacttgtag gccgcgatgc tgccggagat gcagaggacg atttccatct
      781 tgttcaggga acgaccccgg agggcgggct cagtcttcct tatccttctt cttgctcttc
      841 ttctttttct cggtgtcttc gtcttcctcg tcgtcgtcct tcgctttcgc cttttctttc
      901 cctttttctt tttccttctc gtcttcgact tccagggcct tcaggttcaa ttcgagcggt
      961 tttccgtcgg atttcgggac cggcggcagt ttttcgatct cctccaggga gacgcctccc
     1021 gccaagatct ccttgagagc catattgatc agcaccggga ccggttccgt gctctgatcg
     1081 cgtttcttga tctcctgagc ccaacgcagc gccgtcttga tcaaccgata tttatcctgt
     1141 ccgacatcca gcatcaattg cgtcaagctc cagttctcct tcgacacctt ttcttccttc
     1201 tttttctgtt cgctcacgag gaatcctcct attttgtcag acaaagaaat ttttcggagt
     1261 agcggcacac ccggtgatgt tcggcccgga tgatggcgag gaaatcgttc atggcctcgc
     1321 ggatgtcgtt gttgaccacc aggtaattgt aatgctgaag gtaggtcagt tcgtgccgcg
     1381 cgttggaaag acgtttatgg atggtctcct cgtcgtcctg gccgcgcgtg cgcaatcggc
     1441 gttcgagcga ctcccagttg ggcggataga cgaagatgct gacgcactgg ggatatttat
     1501 cctggatggc cagggcgccc tgcgggtcca cgtcgaggag gatgtcggcc ccccgggcca
     1561 gctgatcgtc gatgaacgaa cggggcgtgc cgtaatagtg gtcgtgcacc agggcccatt
     1621 ccaggaattc gccttcgtcg atcttcttct tgaactggtc caccgtgacg aagaaatagt
     1681 ccttgccgtt cacttcggag acgcgcgccg ggcgggtggt ggccgagacg gaatagacca
     1741 aatcggcccg ttccttcagg agggcctggc acaaagtcga tttccc
//
LOCUS       DAOTFD010000342         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_60604_length_1772_cov_4.278393, whole genome shotgun sequence.
ACCESSION   DAOTFD010000342 DAOTFD010000000
VERSION     DAOTFD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60604_length_1772_cov_4.278393"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1212
                     /locus_tag="P5079_11950"
     CDS             82..1212
                     /locus_tag="P5079_11950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY30735.1"
                     /translation="MRVALVHDWLTGMRGGEKVFEVLCGIFPEADVFTLVHIPGKVSP
                     AIERHPIKTSFIQKLPLAFSRYRHYLPLMPLAAERFDLSGYDMVVSSSHCVAKGVRVP
                     EGVPHVCYCHTPMRYLWDQYEEYFGAGRASAPVRAAMRLLRPRLQRWDVDTARGVSRF
                     IANSRNVAGRIRRHYGREAEVIHPPVDTQRFRTSDRDDGYYLVVSALVPYKRVDVAVE
                     AFTLSGKPLKIVGSGPEERSLRAAAGRNIEFLGWADDEKLADAYAGCRALIFPGEEDF
                     GIVPLEAMASGKPVLALGKGGALETVRPLTEGELSPTGLFFDELSAATLNNTVDFFES
                     ARRRFHADAIRRHAETFDVELFRQKIQNYLMTAANEAGGPRR"
     gene            1209..>1772
                     /locus_tag="P5079_11955"
     CDS             1209..>1772
                     /locus_tag="P5079_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30736.1"
                     /translation="MTPSLLSVLAFLMGFTPPLSIAAASAAWLLFALVWLVRLFSGSE
                     TLTFKTTPLDKPILAFLAAWLISALFGLAPLDSLRELPSQLRFVIFYLLLWVSGGRYV
                     SAAVKGYFWGVGVAVGYGFLQFGLSRLVLPETQAAWLSTLGEKTVRYLFPAVGRVHGS
                     VHPLTFAEMLLPGLFFAVVVYLNSARWR"
BASE COUNT          274 a          559 c          565 g          374 t
ORIGIN      
        1 caagggcctg gagcgggcgg cgatgtattc ctggcggatt accgccgacg agacgttcca
       61 ggtctaccgc gaggtgttgt catgagagtc gctctggtgc acgactggct caccgggatg
      121 cgcggcggtg aaaaagtgtt cgaagttctc tgcggcattt ttcccgaagc ggacgtgttc
      181 accctggtgc acatccccgg caaagtttcc ccggccatcg aacggcatcc catcaaaacc
      241 tctttcattc aaaaacttcc cctggccttt tcccggtacc gtcactatct tccgctcatg
      301 ccgctggcgg cggaacggtt cgacctgtcg ggctacgaca tggtggtctc ttccagccat
      361 tgcgtggcca agggggttcg ggtcccggaa ggggtcccgc acgtctgcta ctgccacacg
      421 ccgatgcgtt acctctggga ccaatatgag gaatatttcg gggcgggccg cgcctcggcc
      481 ccggtgcgcg ccgcgatgcg gctgctgcgg cctcgcctgc agaggtggga cgtggacacg
      541 gcgcgggggg tgagccggtt catcgccaat tcccgcaacg tggccggccg catccgacgg
      601 cattacggcc gggaagcgga ggtgatccat ccgccggtag acacccagcg attccgaacg
      661 agcgaccggg acgacggcta ctatctggtg gtttcggctc tggtgcccta caaacgagtg
      721 gacgtggccg tggaggcgtt cactctttcg gggaaaccgc tcaaaatcgt gggaagcgga
      781 ccggaagagc ggagtctgcg cgccgccgcc gggcgcaaca tcgaattttt aggatgggcg
      841 gacgacgaga agctggcaga cgcctacgcc ggctgccggg cgctgatctt cccgggggag
      901 gaagatttcg gcatcgtgcc gctggaggcg atggcctccg gaaaacccgt cctcgcgctc
      961 ggcaaaggcg gcgcgctgga gacggtgcgt cccctgacgg aaggagaact gagtcccacc
     1021 ggtcttttct tcgatgagct ttcggccgcc acattgaaca acacggtgga ttttttcgaa
     1081 tccgcccgcc gccggttcca cgcggacgcc atccgtcgcc acgcagaaac cttcgacgtt
     1141 gagttgttcc gccaaaagat tcagaattac ctcatgaccg ccgccaacga ggccgggggc
     1201 ccccgacgat gacgccgtcc ctgctgtccg tcctggcctt tctcatgggt ttcacgccgc
     1261 ctctctccat cgccgccgcc agcgcggcct ggttgctctt cgccctcgtt tggctggtgc
     1321 ggttgttctc cggctcggag accctcacct tcaaaacgac tcccctggac aaacccatcc
     1381 tggcctttct ggcagcctgg ttgatctccg ctctgttcgg cctggcaccg ctcgacagcc
     1441 tgcgggagct tccctcgcaa ctgcggttcg tgatttttta tctcttgttg tgggtgtcgg
     1501 gcgggcgtta cgtgtcggcg gccgtgaaag ggtacttctg gggcgtgggg gtggcggtcg
     1561 gatacggttt tcttcagttc ggtctttccc gcctcgtcct gccggaaacc caggccgcct
     1621 ggttgagcac cctgggagag aagacggtcc gctatctgtt cccggcggtg ggacgggtgc
     1681 acggttcggt gcatccgctg acgtttgccg agatgctgtt gcccggactt tttttcgccg
     1741 tggtcgtcta cctgaattcc gcccgctggc gc
//
LOCUS       DAOTFD010000343         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_61735_length_1754_cov_2.965862, whole genome shotgun sequence.
ACCESSION   DAOTFD010000343 DAOTFD010000000
VERSION     DAOTFD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61735_length_1754_cov_2.965862"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="P5079_11960"
     CDS             <1..885
                     /locus_tag="P5079_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRY30737.1"
                     /translation="SGLHDVVMVIGAERMREVNNLEATDFVATLTHPLAEYIYGVTLP
                     SLAGMFTRLYMEKYGVTSRHLAMVAVKNHDNALLNPFAHIHQKITLEGILDSPEAMTN
                     NPYVAEPLRFFDCCPVSDGGACLILVSEEEAKKLKKPLVRLAGVGQATDTHTVQERRD
                     PTDLLAVRAAAAQAFAMAKVKPSDVGVAELHDAFTILEIVESEEVGFFKKGEGHRALE
                     RGETRLDGRIPINTSGGLKGKGHPVGATGVGQAHEIVTQLRGEAKKRQVKNARVGFTC
                     NFGGFGNNVVCLVFVREG"
     gene            888..1295
                     /locus_tag="P5079_11965"
     CDS             888..1295
                     /locus_tag="P5079_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRY30738.1"
                     /translation="MDIYAYKCRKCGHVNYPYRLVCKKCRGNEHEEFDPVPLPKKGRL
                     LTFTRLHTLPADFLVATLSLGIVELENGVRITGQLNIPEPAIGMKVKGEVEPVRTDEF
                     RKHYGMVFSAEEGPAGSLRRSRPVGKRSRRPPH"
     gene            1454..1754
                     /locus_tag="P5079_11970"
                     /pseudo
     CDS             1454..1754
                     /locus_tag="P5079_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362279.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
BASE COUNT          357 a          516 c          574 g          307 t
ORIGIN      
        1 tccggtctcc acgacgtggt gatggtcatc ggcgcggaac gcatgcgcga ggtgaacaac
       61 ctggaagcca cggactttgt ggcgaccttg acccatcccc tggcggaata tatttacggg
      121 gtgacgctcc cttccctggc cggaatgttt acccggctct acatggagaa atacggtgtg
      181 acttcccggc acctggcgat ggtggcggtg aagaaccacg acaacgccct gttgaacccg
      241 ttcgcccaca tccaccagaa gatcacgctg gaagggatcc tggattcccc cgaggcgatg
      301 accaacaacc cgtacgtggc cgaacccctt cggttcttcg actgctgtcc cgtttccgac
      361 ggaggggcgt gcctcatcct ggtgagcgag gaggaggcca aaaaactcaa gaagccgttg
      421 gttcggctgg cgggcgtggg gcaggccacg gacacgcaca ccgtgcagga acggcgggac
      481 ccgaccgatc tcctggccgt gcgggccgcg gcggcgcagg cgttcgccat ggccaaagtg
      541 aaaccgtcag acgtcggcgt ggccgaactc cacgacgcct tcaccatcct ggagatcgtg
      601 gagagcgagg aggtcgggtt cttcaaaaaa ggggagggac accgggcgct ggaacggggc
      661 gagacccgcc tcgacggacg gatccccatc aacacctcgg gggggctcaa aggcaaaggc
      721 catcccgtcg gcgccaccgg ggtggggcag gcgcacgaga tcgtcacgca actgcgcggt
      781 gaggcgaaga aacgccaggt gaagaacgcc cgcgtcgggt tcacctgcaa tttcggcggg
      841 ttcggcaaca acgtggtttg tctggtcttc gtccgggagg gctgatcatg gacatttacg
      901 cctacaagtg ccggaagtgc gggcacgtca attatcccta ccgcctggtc tgcaagaaat
      961 gccggggcaa cgagcacgag gagttcgatc ccgttcccct gccgaagaag gggaggctcc
     1021 tgaccttcac ccggctgcac acgttgccgg cggatttcct ggtggccacg ctctccctcg
     1081 gcatcgtgga actggagaac ggcgtgcgca tcaccgggca gctgaacata ccggaaccgg
     1141 cgatcgggat gaaggtcaag ggcgaggtgg agccggtccg cacggacgag ttccggaagc
     1201 attacgggat ggtgttttcg gcggaggagg gcccggcggg gagccttcgg cgctcccggc
     1261 ccgtcggcaa acgatcccgg cggccgccgc attgacggcc gaaaagaaaa gtcctataat
     1321 gaaaaagtaa ctttcctctc gtattttcaa cccatagaca cgatctgaaa aaacaagagg
     1381 gcgataaagt cggtccgcac ctgacggcgt gggcttttat ataaatcata ttccttacgg
     1441 aggaggcttc cttatggcag tgcgcgttgc gatcaatggt ttcggccgga tcggccggtt
     1501 ggttttccgg gctcttgtgg accaaggcct gttgggcggc aagttccagg tggtggcggt
     1561 gggggacatc gtgcccgccg acaacctcgc ctacctttta cagtacgact ccacccaggg
     1621 ccggttcaag ggacaggcgt cgtccaagaa atcctccgcg gataagacgg acgacgacat
     1681 cctggtcgta acggcaacga catccgcgtg gtgagcgcga acgtcacctg gtcggcgtcg
     1741 ctctggcgat cggc
//
LOCUS       DAOTFD010000344         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_61838_length_1752_cov_3.650560, whole genome shotgun sequence.
ACCESSION   DAOTFD010000344 DAOTFD010000000
VERSION     DAOTFD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61838_length_1752_cov_3.650560"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="P5079_11975"
     CDS             <1..983
                     /locus_tag="P5079_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRY30739.1"
                     /translation="QLDQVKKHGVREVVLALGYQASHFQRHLGDGRRWGMRFIYSLEK
                     EPLGTGGAIRKALPFLKGTTFILNGDVLSDVDLTALLSFHRRNKADGTLALMAVKDPS
                     SYGLIETEESGKIKKFVEKPSAEEMNVNTINAGFYVFEENVVRQIPANQPVSIERDVF
                     PKLVKNGFSLYGFPHRGYWSDIGTLKSYWDTHMDLMQDFSGSPDKSIKKLQKGIWGGT
                     KCRLDTPLKLEGTLLMGHRCRLGPNVTVRGCVSLGDGCTIAENVILTDCVVLEKTHVG
                     VNATLENCIVGENCHIGSFCHVGPGTVLANRSTLEPYTKQISGLSREPHA"
     gene            976..1344
                     /locus_tag="P5079_11980"
     CDS             976..1344
                     /locus_tag="P5079_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY30740.1"
                     /translation="MLKLVKKPWGNEIWFACAPKYVGKLISVRKGHRLSLQYHRRKDE
                     TMFLEKGRCLIELNGRKKLFKSGDTIRIKPGTVHRLDARYGNIRIIEVSTPQVRDVVR
                     LEDDYHRAKHIISRKRGARK"
     gene            1341..>1752
                     /locus_tag="P5079_11985"
     CDS             1341..>1752
                     /locus_tag="P5079_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRY30741.1"
                     /translation="MIHIRFGTDGWRAIIGEEFTFANVRRVADALGQVLPQRSSVVIG
                     YDYRFLSDSFALEAGAVLASQKHRVTLLSHATTTPALSFAVRALRAKAGVMISASHNP
                     ALFNGVKIKLYPGHSADPEFTRQVEDAVRPEVPHA"
BASE COUNT          422 a          472 c          487 g          371 t
ORIGIN      
        1 atcagctgga ccaggtcaaa aaacacggcg tccgagaagt tgtcttggcc ttgggttatc
       61 aggcgagcca tttccagcga catttgggcg atgggaggcg atggggaatg cgattcatct
      121 attctctgga aaaagaaccg ctgggaaccg gtggcgccat ccgaaaagca ttgcctttcc
      181 tcaagggaac gaccttcatc ctgaacggcg acgtattgag cgatgtggac ctcacggctc
      241 ttttgagttt ccatcgccga aacaaagccg acggaacgtt ggccctcatg gcggtgaaag
      301 acccgagcag ctacggcttg atcgaaaccg aagaaagcgg aaagatcaaa aagttcgttg
      361 aaaaaccttc cgccgaggaa atgaacgtaa ataccatcaa cgcgggattt tatgtttttg
      421 aagagaacgt ggtgcgacag attccggcaa accagccggt ctccattgaa cgcgatgtct
      481 ttccaaaact ggtaaagaat ggtttttctc tgtacggttt tccgcatcgg ggctattggt
      541 cggacatcgg cacgctgaaa tcctattggg acacccacat ggatttgatg caagatttct
      601 ccggctcccc ggacaaatcc ataaaaaaac tccaaaaagg catctggggg ggcacaaaat
      661 gccggttgga tacgcccctc aaactcgaag gcacattgct catgggccac cgttgccgcc
      721 tcggcccgaa cgtcacggtg cgcggatgtg tcagtttggg ggacggatgc acaatcgccg
      781 aaaatgttat cctgacggac tgcgtggtgc ttgagaagac acacgtcggc gtcaacgcca
      841 ccctcgagaa ttgcattgtc ggtgaaaact gtcatattgg ctccttctgt catgtcggtc
      901 ccggcacggt attggcaaac cgctcgacgc tggagcccta caccaaacag atcagcggtc
      961 tttcccggga gccccatgct taaactcgtc aagaagcctt gggggaacga gatctggttc
     1021 gcctgtgctc cgaaatatgt gggcaagctg atttctgtcc ggaagggaca ccggctttct
     1081 ctgcaatatc atcgacggaa agacgagacc atgtttctgg aaaaaggacg ttgccttatc
     1141 gaattgaacg gccgaaaaaa actttttaaa agcggcgaca ctatccgcat taagcccggc
     1201 acggtgcacc gattggacgc gcgttatggg aatatacgca tcattgaagt ctccacgccg
     1261 caggtgcggg acgttgtgcg gctggaggac gattaccatc gcgcgaaaca cattatttca
     1321 cggaaaagag gagccagaaa atgatccaca tacgattcgg aacggatggc tggcgagcca
     1381 tcatcgggga agaattcact tttgccaacg tccgccgtgt cgcggacgcc ttgggacagg
     1441 tgttgccgca gaggtcctct gtcgtcatcg gttacgacta ccggtttctt tcggacagtt
     1501 tcgccttgga ggccggggcg gttctggctt cacaaaaaca ccgcgtcact ctgctctccc
     1561 atgccacgac cacgccggcg ctttctttcg ccgtccgcgc cttgcgggcg aaagcggggg
     1621 tgatgatttc cgcaagccac aacccggcgc tgttcaacgg ggtgaaaata aaactctacc
     1681 ccggccattc ggcggacccg gagttcaccc gacaggtcga ggacgccgtc cggccggaag
     1741 tgccccatgc ct
//
LOCUS       DAOTFD010000345         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_65436_length_1697_cov_4.227162, whole genome shotgun sequence.
ACCESSION   DAOTFD010000345 DAOTFD010000000
VERSION     DAOTFD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65436_length_1697_cov_4.227162"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1697)
                     /locus_tag="P5079_11990"
     CDS             complement(<1..>1697)
                     /locus_tag="P5079_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30742.1"
                     /translation="AQQAVTILPAPARKIVVTSLPQTLVAGTTSQAFTFQLQDSFGNP
                     SPVSSLLGNVEDSVGQGVSFELTSNSTGTVMFASPNNDPLAFTVTTGSAKMVVGQHTT
                     TFYMIDTWVGQHEVTVDQVKVHGWTVAVQTYTVTPAPPTVLAFINPPRKMIASSTATV
                     TYAVGVTSPVFTVQLRDSFGNISFSTSEIQAHVQSNSPAGRGSWDNVGFSPINAPSNS
                     LILTYSPGQTESSFYYRDTVAGISTVTVSVPFSVFTSTYQPVVVTPNQAARFVINHPY
                     GLSSPLSVRIFGTISVTAKDAYNNVANGGQGIFFDGAYQNNGLSYAGTAAFGHNGSTA
                     TVTIQPSSKTVTEADKGTFTFQVLDTQQSSLLLSSSDTVRTSIVGTTADSGSSGDVVT
                     SGLVLLADTANSDLSPEPEPRSFLKNLVGVASTLFQGDGESTASPNPIAMLRLRVGVR
                     PLTVATTAQWEALRVEKLGTVNPDQITEVGLWQDLNGDGTFDALFDGRVVSGLNEPGV
                     PVSTSVFITTVVPVAATLDFTSNPQIVSTTEKIFFLTIRVSTVATPGTTIGYRVLGA"
BASE COUNT          278 a          494 c          632 g          293 t
ORIGIN      
        1 cgcgcccagg acccggtagc cgatggtggt gccgggcgtg gccacggtgg agacgcggat
       61 ggtgaggaag aaaatctttt ccgtggtgct gacgatctgc gggttggacg tgaaatcgag
      121 cgtggccgcc accggcacca ccgtggtgat gaacaccgag gtgctcaccg gcacccccgg
      181 ctcgttcagg ccgctgacca cgcgcccgtc gaagagggcg tcgaaggtgc cgtcgccgtt
      241 caagtcctgc cagagaccga cctccgtgat ctggtcgggg ttcaccgtgc ccagtttctc
      301 cacccgcagg gcctcccact gggcggtggt ggccacggtg aggggccgca cgcccacgcg
      361 cagacgcagc atggcgatgg ggttgggcga ggccgtggat tcgccgtcgc cctggaacag
      421 ggtgctcgcc acgcccacca ggttcttcaa gaaactgcgc ggttcgggtt cgggcgacag
      481 gtcggagttg gcggtgtcgg ccagcagcac caggcccgag gtcaccacgt ccccgctgga
      541 gccgctgtcg gcggtggtgc cgacgatgga ggtcctcacc gtgtcgctgg aggagagcag
      601 gagggaggac tgttgggtgt ccagcacctg gaaggtgaag gtccccttat ccgcctcggt
      661 cacggtcttg ctggagggct ggatggtgac ggtggcggtg gagccgttgt gtccgaacgc
      721 cgccgtgccc gcgtaggaga gcccgttgtt ctgataggcc ccgtcgaaga agataccctg
      781 gccgccgttc gccacgttgt tgtaggcgtc cttggcggtc acgctgatgg tgccgaagat
      841 gcgcacgctc agaggcgacg aaagtccgta cggatggttg atgacgaacc tcgccgcctg
      901 gttcggcgtc accaccacgg gctggtaggt ggaggtgaac acgctgaacg gcacggagac
      961 ggtgacggtg gagatgcccg ccacggtgtc gcggtaatag aaactgcttt ccgtctggcc
     1021 tggggaatag gtcagtatca gcgagttgct gggcgcgttg atgggcgaga aacccacgtt
     1081 gtcccaggac ccgcggcccg cgggggagtt cgactgcacg tgcgcctgga tctccgaagt
     1141 ggagaaggaa atgttgccga aggagtcgcg caactgcacg gtgaacaccg gggaggtgac
     1201 gcccaccgcg taggtcacgg tggcggtgga ggaggcgatc atcttccgcg gcgggttgat
     1261 gaaggccagc accgtgggcg gcgccggcgt gaccgtgtag gtctgcaccg ccacggtcca
     1321 gccgtggact ttcacctggt ccacggtgac ctcatgctgg cccacccagg tgtcgatcat
     1381 gtagaaggtg gtggtgtgct ggcccaccac catcttggcc gagccggtgg tgaccgtgaa
     1441 cgccagcggg tcgttgttgg ggctggcgaa catcacggtg ccggtggagt tggaggtcag
     1501 ctcgaaactc acgccctgcc ccacgctgtc ctccacgttg cccaggaggg agctgacggg
     1561 gctggggttg ccgaaggagt cctgcaattg gaacgtgaag gcctgcgagg tggtcccggc
     1621 caccaaggtc tgcggcaggg aggtgaccac gattttacgc gccggcgccg gcaggatggt
     1681 gacggcctgc tgggcgg
//
LOCUS       DAOTFD010000346         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_66480_length_1681_cov_4.600861, whole genome shotgun sequence.
ACCESSION   DAOTFD010000346 DAOTFD010000000
VERSION     DAOTFD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66480_length_1681_cov_4.600861"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="P5079_11995"
     CDS             complement(<1..990)
                     /locus_tag="P5079_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30743.1"
                     /translation="MSRRLGLLVAGAALLLFALWTFWLRPPLARGIHRLCESRLPPLI
                     GRPVTLEEVRIHPLFLSVTLQGLRIGEAEHPVFVCRRWVLRPRPFHLERDFPWLKPAI
                     GNSFVDGPVLRPETAAGPSGKAPALSQMLTTLGGRVAWLRHPLRWNHGTVHLPAAADR
                     EGLSVSDLSGHFDWNRRGVVLHARGDVPFGDARFSLHVAPEGTWKLSIVSEKIDLGKL
                     PLRGGQGRLAGVLSGKGEAQGTVAQWDAKNWKSIRWRIAGNVSNGSWSPPNSTLRIPF
                     EGTFGLDRDKLALSHFKLLKKISVEGHCSNPFGPDARMDFSLRGQKMDTSGLLS"
     gene            complement(1133..>1681)
                     /locus_tag="P5079_12000"
     CDS             complement(1133..>1681)
                     /locus_tag="P5079_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY30744.1"
                     /translation="DGVLTDNLGHKVNFKNTVIIMTSNVGARLISKGKSLGFLVQEDA
                     QKDYAAMKDTVMEEVKKAFNPEFLNRLDEIIVFHSLTREDSRKILDLMLKKVEKKLGS
                     QGFSLILSEEAKNFLVETGFDPNYGARPLQRCIQRQVEDPMAEEILAKRIPSGGKVYV
                     DLDADAKKLTFSTAPAEKPVKK"
BASE COUNT          304 a          473 c          581 g          323 t
ORIGIN      
        1 ggacaataac ccagaggtat ccattttttg tccacgcagg gaaaagtcca tgcgggcgtc
       61 cgggccgaag gggttggagc aatggccttc caccgatatt tttttcagga gtttgaaatg
      121 ggacaaggcg agcttatcgc ggtcgagtcc aaaagtcccc tcgaagggga tgcggagggt
      181 ggagttcggc ggggaccagg agccgttcga cacattcccg gcaatccgcc agcggatgga
      241 tttccagttt ttcgcgtccc actgggccac cgtcccttgc gcctccccct tcccgctcag
      301 aacgccggcc agcctcccct gtccccctcg aagagggagt tttccgaggt cgatcttttc
      361 ggagacgatc gacagtttcc aggttccttc cggagccacg tgcagagaga aacgcgcgtc
      421 gccgaacggg acgtctcctc gggcgtggag caccaccccc cggcggttcc agtcgaaatg
      481 gccggagaga tccgaaacgg acaggccttc gcggtcagcg gccgcgggca ggtgcaccgt
      541 gccgtggttc catcggaggg gatgtcggag ccaggcgacc cggccgccga gagtcgtcag
      601 catttgggag agggccggtg cttttccgga ggggccggcg gccgtttctg ggcgcaggac
      661 gggaccgtcg acgaaagagt tcccgatggc cggtttcagc cagggaaaat cacgttccag
      721 gtgaaagggc ctgggacgca ggacccatcg tcggcagaca aagaccggat gttcggcctc
      781 gccgatacgt agtccctgga gcgtgacgga aaggaacaga gggtggatgc gcacctcttc
      841 caaggtcacc ggccggccga tgagtggagg aaggcgggat tcgcacagcc ggtggatccc
      901 acgcgccagc ggcggccgca accagaaggt ccagagtgcg aagaggagga gggcggctcc
      961 ggcgacgagg aggccgagtc ggcgggacat cagaaacggg ctcctttcgg acccggggcg
     1021 gccggggagg acagcgcggc cgttacggga cgatcatggc gttgcgggag tggtccgtca
     1081 accatccgga ggggcggaag ccaaaggcgg aagcggtcgg tctccggtga aattattttt
     1141 tcaccggttt ttcggccggg gctgtcgaga aggtcagttt tttcgcgtcg gcgtccaggt
     1201 cgacgtagac tttgccgccg gagggaatgc gtttggcgag gatctcctcg gccatcgggt
     1261 cctccacctg gcgttggatg caccgttgaa gcggccgggc gccgtagttc gggtcgaaac
     1321 cggtctccac caggaagttc ttcgcctcct ccgagaggat gagcgagaac ccctggctcc
     1381 cgagtttttt ctccactttt ttgagcatca ggtccaggat cttgcggctg tcctctcgcg
     1441 tgagcgaatg gaagacgatg atctcgtcga gccggttgag gaactcgggg ttgaaggcct
     1501 ttttgacctc ctccatcacc gtgtctttca tggcggcgta atccttctgg gcgtcctcct
     1561 gcacgaggaa gccgagggat tttcccttgg agatgaggcg ggcgccgacg ttggaggtca
     1621 tgatgatgac ggtgttcttg aagttcacct tgtggccgag gttgtcggtg aggacgccgt
     1681 c
//
LOCUS       DAOTFD010000347         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_68946_length_1646_cov_3.203017, whole genome shotgun sequence.
ACCESSION   DAOTFD010000347 DAOTFD010000000
VERSION     DAOTFD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68946_length_1646_cov_3.203017"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="P5079_12005"
     CDS             complement(<1..1042)
                     /locus_tag="P5079_12005"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30745.1"
                     /translation="MKNRETQMKEAFFSARKAILASLLFAFTTPLFAKGPGTSTANFL
                     KMGVGGRGVAMGEAQTAAVNDVMALYWNPAALGILRQNEVGFMHNSYLQGIGQDVLYY
                     AHPSQNAGTFGAGVSLLRVGEITGYDTSGAKTQELTASDMLVSLGWGKTWENMVLLPG
                     LNTGLNVKFLQKKLGDDSASCVMGDVGFLYEVQEENFLSGLRTGIAMQNLGGGLNFLE
                     EKSNLPVLTKVGFAYPFFGETFTLAADLVSPSDNDLYGNFGLDYRLWDILAFRVGYKG
                     QHDTDNGLTYGVSFGNERFHLDYAFVPFGDLGDSHRVSVGLRFGNTYRKTQVQAQLRQ
                     AYEKAEARYAQGY"
     gene            complement(1236..>1646)
                     /locus_tag="P5079_12010"
     CDS             complement(1236..>1646)
                     /locus_tag="P5079_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRY30746.1"
                     /translation="RIYWLDEENGVWVRTPGSTVDKVNNQVSVNVRHFSVYAIIGAPS
                     LDLSGAYAYPVPFKPSKGHKTITFTNLSSIADIKIYTVNGELVRELKETDGDGQLKWD
                     PVANEKGEPLGSDVYLYVIENNEQKKIGKLMIVR"
BASE COUNT          317 a          442 c          501 g          386 t
ORIGIN      
        1 gatatccctg ggcgtaacgc gcctcggctt tttcataggc ttgccggagc tgggcctgca
       61 cctgggtctt gcggtaggtg ttgccgaaac ggagtccgac gctcacccgg tggctgtcgc
      121 ccaggtcccc gaacggaacg aaggcgtaat cgaggtgaaa acgttcgttg ccgaagctca
      181 cgccgtaggt gaggccgttg tccgtgtcgt gctggccctt gtatcccacg cggaacgcca
      241 ggatgtccca gaggcggtaa tccagcccga agttgccgta aaggtcgttg tcgctggggg
      301 agaccaggtc ggcggcgagg gtgaaggttt cgccgaagaa agggtaggcg aacccgacct
      361 tcgtgagaac cgggaggttc gatttttctt ccaggaaatt gaggccgccg ccaaggttct
      421 gcatcgcgat gccggtgcgg agcccgctca ggaaattctc ttcttggact tcatacaaaa
      481 agcccacatc ccccatcacg caggaggcgg aatcatcgcc cagctttttt tgcaggaatt
      541 tcacgttgag gccggtgttc aggccgggaa gaaggaccat gttttcccag gttttccccc
      601 agcccagact caccagcatg tcggaggcgg tcagttcctg ggttttggcg ccggaggtgt
      661 cgtagccggt gatctctccc acccgcaaaa gggagacgcc ggcgccgaaa gtgcccgcgt
      721 tttgggaagg gtgggcgtag taaaggacat cttggccgat gccctgcaga tagctgttgt
      781 gcatgaaccc cacttcgttt tgccggagga tgcccaacgc ggcggggttc caatagaggg
      841 ccatcacatc gttcaccgcg gcggtctggg cttctcccat ggccacgccc cgtcccccca
      901 cccccatttt gaggaagttg gcggtgctgg tgccgggtcc cttggcgaag aggggggtcg
      961 tgaaagcaaa aaggaggctg gccagaattg cttttctggc gctgaaaaaa gcctctttca
     1021 tttgcgtttc acggtttttc atggccgttt cctcattatt tcctttcttt ttagcccaca
     1081 aatgcctttc cacacttcac tttcatgcaa catttcggcc actggtggag ggtttaaaga
     1141 gaatataatg aggtttttta agaataaacg ggaacagctg gaaggagaat cgcggttcaa
     1201 aacagcagga aatatcttta gataaaaagc ctcggttatc ggacgatcat cagttttccg
     1261 attttctttt gttcgttgtt ctcgatgacg tagagataca cgtcgctgcc gagaggttcc
     1321 cccttctcgt tggccaccgg gtcccatttc aactggccgt cgccgtcggt ttccttcagt
     1381 tcgcgcacca gttcgccgtt cacggtgtag atcttgatgt cggcgatgct ggaaaggttg
     1441 gtgaaggtga tggtcttgtg gcccttggag ggcttgaagg gcaccggata ggcgtaggcg
     1501 ccggaaaggt ccagcgacgg ggccccgatg atggcgtaga cgctgaagtg gcgcacgttg
     1561 acgctgacct ggttgttgac cttgtccacc gtgctgccgg gggtgcggac ccacacgccg
     1621 ttttcctcgt ccagccaata gatgcg
//
LOCUS       DAOTFD010000348         1624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_70548_length_1624_cov_4.242192, whole genome shotgun sequence.
ACCESSION   DAOTFD010000348 DAOTFD010000000
VERSION     DAOTFD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70548_length_1624_cov_4.242192"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="P5079_12015"
     CDS             complement(<1..792)
                     /locus_tag="P5079_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRY30747.1"
                     /translation="MSPRPTLYLIDANAYVHRAYHALPPLTNSHGEPVQALYGFARML
                     QKLLRQENPDHIVVCFDTPAPTFRHKAFAAYKANRKETDDALKFQLPLARQMAEAWGF
                     PVVAVEGFESDDLIATLADKAAKKGMNAVVVTGDKDALQLVNEHISVLNEFQSVTYHP
                     ADVEKKYGLRPDQLVDYFALTGDASDNVPGVPGIGPKTATQILHQFGTLDDALARTRE
                     MKDTLRNKLEEHREDALLSRDLVVLNRKVPVPLEPSECLRKTPDRP"
     gene            complement(836..1354)
                     /locus_tag="P5079_12020"
     CDS             complement(836..1354)
                     /locus_tag="P5079_12020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY30748.1"
                     /translation="MRRLRRLFLAVTLAALGGCGAGPKSASPSADPSATGAPAFTLKD
                     IHGKKVSLSDFRGKAVFLDFWATWCPPCRFSIPAIEDLHREFAPRGLNIVGISLDEDP
                     SVVPEFVKENGMNYTVLLGAGSDVADRYQVRGIPAMYLIDKKGNIVRRWIGFDPRLRE
                     EWRAAVDALLKS"
     gene            complement(1351..1611)
                     /gene="trxA"
                     /locus_tag="P5079_12025"
     CDS             complement(1351..1611)
                     /gene="trxA"
                     /locus_tag="P5079_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY30749.1"
                     /translation="MLVDFWAPWCGPCRMLAPIVAELAKEYEGRILVAKLNTDDNPAT
                     ASRFNISAIPTMLFFKGGKVVDQVVGVHNKAEIQGKLDNLLK"
BASE COUNT          265 a          476 c          577 g          306 t
ORIGIN      
        1 ggggcgatcc ggggtttttc ggaggcactc cgacggctcc agggggaccg gaaccttgcg
       61 gttgagcacg accaggtcgc ggctgaggag ggcgtcttcg cggtgctcct ccagcttgtt
      121 ccgaagggta tccttcattt cccgggtgcg ggccagggcg tcgtcgaggg tgccgaactg
      181 atgcagaatc tgcgtggcgg ttttcgggcc gatgcccgga accccgggca cgttgtcgct
      241 ggcgtctccg gtgagggcga aatagtccac cagttggtcg gggcggaggc cgtatttctt
      301 ctccacgtcg gccgggtggt aggtcacgct ctggaattcg ttgaggacgg agatgtgttc
      361 gttgaccagt tggagggcgt ctttgtcgcc cgtgaccacc acggcgttca tccccttttt
      421 cgcggccttg tccgcgaggg tagcgatgag gtcgtcggat tcgaacccct ccaccgctac
      481 cacggggaac ccccacgcct cggccatctg ccgggccagg ggcagttgaa acttgagggc
      541 gtcgtccgtc tccttgcggt tggccttgta cgcggcaaaa gccttgtggc ggaacgtggg
      601 ggcgggcgta tcgaaacaga cgacgatatg gtcggggttc tcctggcgca ggagtttctg
      661 cagcatgcgg gcgaagccgt agagggcctg cacgggctct ccgtgggagt tcgtgagggg
      721 cggcaaggcg tggtacgccc ggtggacgta ggcgttggcg tcgatgagat agagggtcgg
      781 gcgggggctc acggtcggcg gggaaagacg ggggcggcgg gaggcgcggc cggggtcagg
      841 atttcagaag ggcgtcgaca gcggcgcgcc attcctcccg caggcgcgga tcaaagccga
      901 tccagcgacg gacgatgttg cctttcttgt cgatgagata catggccggg atcccccgca
      961 cctggtaacg gtcggccacg tcgctgccgg cccccaaaag cacggtgtag ttcatgccgt
     1021 tttcctttac gaactccggc acgacggacg ggtcctcgtc gagactgatg ccgacgatgt
     1081 tcagtccgcg cggagcgaat tcccgatgca gatcctcgat ggccgggatg gagaaacggc
     1141 acggagggca ccacgtggcc caaaaatcca gaaaaaccgc ctttccccgg aaatccgaca
     1201 gggagacctt cttcccgtgg atgtctttca gcgtgaacgc cggcgccccg gtggcggacg
     1261 ggtccgccga cggcgaggcg gacttcggtc cggcgccgca gcctcccagc gccgccaagg
     1321 tcaccgcgag aaaaagccgg cggagtcgtc tcatttgagg aggttgtcca atttcccctg
     1381 gatctcggcc ttgttgtgca cgcccacgac ctggtccacg accttccccc ccttgaagaa
     1441 cagcatggtg gggatggccg atatgttgaa acgggaggcg gtggcggggt tatcgtcggt
     1501 gttgagtttg gcgacgagga ttctgccctc gtattccttc gccagttccg ccacgatggg
     1561 ggccagcatg cggcaggggc cgcaccacgg ggcccagaaa tccaccagca cgggcgtttt
     1621 ggcc
//
LOCUS       DAOTFD010000349         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_71657_length_1610_cov_3.833441, whole genome shotgun sequence.
ACCESSION   DAOTFD010000349 DAOTFD010000000
VERSION     DAOTFD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71657_length_1610_cov_3.833441"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1610
                     /locus_tag="P5079_12030"
     CDS             <1..>1610
                     /locus_tag="P5079_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY30750.1"
                     /translation="FTNILLHAANASLFYLLLVCLLKRLFPGGTEEEFVFPAVLGALV
                     FSIHPLRVESVAWVTERRDVLSGFFSLGTLLAYANYVRLGALGMGRGSQRGWYAGAVV
                     LFGGALLSKSTVAPLAGVLLLLDVYPWRRFQEPGTRWARLLAEKVPFFVLGAAASYGM
                     IQATLAIDSLRSLEHFSVASRGSCVAWSLAYYVWKTAFPVHLSPIYASTGIVHFSEWA
                     FVFWVLIALVTLSAVFLRRRWPGVLAAWGYSLLMLSPSLGIADSVSLMQDRYSYLANL
                     GWSVILASGLLSWRRKTAVGGGRRFFWGGVILSVALLATYATLTIRQLRIWKNSESLW
                     RRALDCNPDSDIANENYGLTLAQSGRHEEAIGYYQKSYAIYPRCPVVVNNMGDALLRL
                     KKPLEALPWFQAALRLRPDSHQTVFNLGVVYFVLNRWTEAVPHFQRALEINSRYAPAH
                     NKLGIAMARLGRWDRAAEHLRDAFLLETRFADDYLKLGDFASRQGLRSEARRIYAEIL
                     EKKPDARDVRARLNALAPDDPRRLRQVF"
BASE COUNT          242 a          432 c          506 g          430 t
ORIGIN      
        1 acttcaccaa tatccttctt catgccgcca acgcgtctct tttctacctc cttctggtgt
       61 gcttgctgaa gcgtttattt cccgggggaa ccgaggaaga gttcgttttc cccgcggtgc
      121 tgggcgccct ggttttttcg atccatccct tgcgggtgga atcggtggcc tgggtgacgg
      181 agcggcggga cgttttgtcc ggattcttct ccctgggcac gttgttggcc tatgcgaact
      241 atgtccgttt gggggccttg gggatgggga ggggttccca aagagggtgg tacgcgggcg
      301 cggtggtctt gttcgggggt gcgctgttgt cgaagtcgac ggtggcgccc ctggcggggg
      361 ttctgctcct cttggacgtc tacccgtggc gtcgttttca agagccggga acgcggtggg
      421 ctcggttgtt ggcggaaaag gtgccgttct ttgtgttggg cgcggcggcc tcgtatggaa
      481 tgatccaggc cacgctggcc atcgacagcc tccgttccct ggaacatttc agtgtcgcca
      541 gccgtggcag ttgtgtggcc tggtccttgg cctattatgt gtggaaaacg gcgttccccg
      601 tgcatctgtc gcccatctat gcctccacgg gcatcgtcca tttttccgaa tgggcttttg
      661 ttttctgggt tttgatcgct ttggtgacgc tttcggccgt tttcttgcga cgacgttggc
      721 cgggggtgtt ggccgcctgg ggctattccc tcctaatgct ctctccgagc cttggcatcg
      781 cggattccgt ttcgttgatg caggaccgtt acagttacct tgctaatttg gggtggtcgg
      841 tgatattggc ttctggtttg ttgtcatggc ggcgaaaaac ggccgtcggc gggggccgcc
      901 ggtttttttg gggaggagta atcttgtccg tcgccctttt ggcgacgtac gccacgctca
      961 ccatccggca gttgcggatt tggaaaaaca gcgagtcgtt gtggcggcga gcgctcgact
     1021 gtaaccccga ttcggacatc gccaatgaaa attatggact cacgctggcg cagtcgggac
     1081 ggcacgagga ggccattggg tattatcaaa aatcctacgc gatttatccg cgttgtccgg
     1141 tagttgtcaa caatatgggg gatgccctcc tgcggctcaa aaaacctctg gaggcgctgc
     1201 cctggtttca agccgccttg cggttgaggc cggattccca ccagactgtt ttcaatttgg
     1261 gtgttgttta tttcgttctc aaccgctgga ccgaagccgt cccccatttc cagcgggcgc
     1321 tggaaatcaa ttctcggtat gctcccgctc ataacaaatt ggggatcgcc atggcccggt
     1381 tgggtcgctg ggatcgggcc gccgaacacc ttcgcgacgc ttttctgttg gagactcgat
     1441 ttgccgacga ttacttgaaa ctgggggatt tcgcttctcg gcagggtttg cgttctgagg
     1501 cacggcggat ctacgctgaa atacttgaaa aaaaacccga cgcccgggat gtgcgggccc
     1561 ggttgaacgc ccttgccccg gatgatccgc gacgcctccg ccaggtgttc
//
LOCUS       DAOTFD010000350         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_75679_length_1559_cov_3.616356, whole genome shotgun sequence.
ACCESSION   DAOTFD010000350 DAOTFD010000000
VERSION     DAOTFD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75679_length_1559_cov_3.616356"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="metK"
                     /locus_tag="P5079_12035"
     CDS             complement(<1..713)
                     /gene="metK"
                     /locus_tag="P5079_12035"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRY30751.1"
                     /translation="MRLDRYVFTSESVTEGHPDKVCDQISDAVLDAVLKDDPTGRVAC
                     ETFVSVGLLIVGGEITTKTYIEVPDLVRGVIRDIGYTDIKYGFNGDTCGVLNAIGRQS
                     PDISQGVDVGGAGDQGLMVGFACDETEELMPSPIMYAHKLTKRLADVRKKNILKYLGP
                     DGKSQVTVEYEGGQPKRIDTVVVSSQHTPDILDKTGKKITNKAREEIIEKVALAVLPK
                     QLLDKDTRFFVNPTGKFVVG"
     gene            complement(775..>1559)
                     /locus_tag="P5079_12040"
     CDS             complement(775..>1559)
                     /locus_tag="P5079_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRY30752.1"
                     /translation="PLFGGLSPEPIEKNLKALVQAVQENKAAIGLAVDGDGDRLGVVD
                     DKGRYLPPHHVFPLILQHLIENRKMKGKVVQTVSLGYLSERIAKKHGVELIEVPVGFK
                     YVVDKMRQTKVFWGGEESGGYGCGLWAWERDGLLSGLLLLEAVMAKHQPLSVLREKLE
                     AEYGKSFFLRNDYAMKNPIADKTAWAETLSHKLPAKLADTKIRELKRLDGLKIVLEDD
                     SWLLLRPSGTEPLLRTYAESPSPEKTKQLLTKAQEIATIKTS"
BASE COUNT          292 a          441 c          438 g          388 t
ORIGIN      
        1 cccaccacga acttgccggt ggggttgaca aaaaaccgcg tgtccttgtc gaggagctgt
       61 ttcgggagga ccgccagcgc cactttttcg atgatctctt cgcgggcttt gttggtgatc
      121 ttcttgccgg ttttgtccag gatgtcgggc gtatgctgag agctgaccac cacggtgtcg
      181 atgcgtttcg gttggccccc ctcgtattcc acggtcactt gggattttcc gtcagggccg
      241 aggtatttca aaatattttt cttgcgcacg tccgccagcc gtttggtgag cttgtgggcg
      301 tacatgatgg ggctgggcat gagttcctca gtctcgtcgc aggcaaaacc gaccatcagc
      361 ccctgatcgc ccgcccctcc cacgtccacg ccttgtgaga tgtccgggga ttgccggccg
      421 atggcgttca atacgccgca ggtgtccccg ttgaacccgt actttatgtc cgtatagccg
      481 atgtctcgga tcacgcctcg gacgaggtcc gggacctcaa tgtaagtttt ggtcgtgatc
      541 tcaccgccga cgatcagaag accgacgctg acgaaggtct cgcaggccac ccggcccgtc
      601 gggtcgtctt tgagaacggc gtccaatacc gcgtccgata tctgatcgca gactttgtcc
      661 gggtgacctt cggtcaccga ttcggatgtg aagacataac ggtcaagtcg catgaaaagt
      721 ccctcctaaa gttgatgcaa gatgagaatt gcccgacagg cggggtaaaa acgtttacga
      781 agttttaatt gtagcaattt cctgggcttt ggtgagcagt tgttttgttt tttccggaga
      841 cggcgattcc gcgtaggttc gcaacagtgg ttctgtgccg ctcggccgaa gaagaagcca
      901 cgaatcatct tccaggacga ttttcaagcc gtcgagccgt ttcagttcgc ggattttggt
      961 gtccgccagt ttggcgggca gtttgtggga aagggtttcc gcccacgccg tcttgtcggc
     1021 gatgggattc ttcatggcat agtcgttgcg gaggaaaaag gattttccgt attcggcttc
     1081 caatttttcc cgaagcacgg agaggggctg gtgtttggcc atcaccgctt ccagcaacag
     1141 gaggcccgac aacaggccat cccgttccca agcccataga ccgcagccgt atccgcccga
     1201 ttcttccccc ccccagaaca cctttgtctg gcgcattttg tccacaacat atttgaagcc
     1261 cacgggaacc tcgatgagtt ccacaccgtg cttcttggcg atgcgttcgg agaggtagcc
     1321 cagagacacc gtttggacca ccttcccctt catctttcgg ttttcgatca ggtgctggag
     1381 tatcaaagga aatacatggt gcggcggaag ataacggcct ttgtcgtcca ccacgccgag
     1441 ccggtcgccg tctccgtcca cggccaaccc gatggccgct ttgttttctt gcacggcctg
     1501 cacgagcgct ttcagatttt tttcaatggg ttccggcgat agcccgccga agaggggat
//
LOCUS       DAOTFD010000351         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_78475_length_1527_cov_5.493886, whole genome shotgun sequence.
ACCESSION   DAOTFD010000351 DAOTFD010000000
VERSION     DAOTFD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78475_length_1527_cov_5.493886"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="P5079_12045"
     CDS             complement(<1..315)
                     /locus_tag="P5079_12045"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRY30753.1"
                     /translation="MSPSPSPSQTKVFKGISASPGIAIGKAFVLEDTDLAVGRWQVPP
                     EAVKAEVARFRVAVADTKKEMLGTQQQILKLLGKSHARLIEAYLLILEDPLINKDVVK
                     NVT"
     gene            complement(324..590)
                     /locus_tag="P5079_12050"
     CDS             complement(324..590)
                     /locus_tag="P5079_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRY30754.1"
                     /translation="MKEAVFIVENKLGLHARPAALFVQTTHKFKCNVSVVRDGQEVDG
                     KSIMGILTLAAEKGAKLTIAADGPDEDALLEQLTQLFKNKFDEE"
     gene            complement(648..1439)
                     /locus_tag="P5079_12055"
     CDS             complement(648..1439)
                     /locus_tag="P5079_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRY30755.1"
                     /translation="MGNGQTLEPVTAAVPAPARLTTGDLWKMFLRSFCIQTLWNFQRM
                     QNLGWLFSLWPALRRFYRPPQRRGVALEHLDYFNTHPYLANLVLGVVAGMEEGAAAGS
                     VRREQIMATKKTMSGPLAALGDTVFWATLRPLAGVLAVALGLTYARLSWWLVPVFFLA
                     AYNSFHLAARVGGLFLGYRHKSEIIHLLRDMRPQKWVRGATLLGAGVSLGALLMLWLA
                     FGRHLGAMAGFISVTLVLLRLRVTSTRVVYAMLAACFCFAYFFGI"
BASE COUNT          326 a          490 c          453 g          258 t
ORIGIN      
        1 cgtgacgttc ttgaccacgt ccttgttgat gagcgggtct tccaggatca acaggtaggc
       61 ctcgatgagg cgggcatggc tcttgccgag cagtttgagg atctgctgtt gcgtgcccaa
      121 catctccttc ttggtgtcgg ccacggccac gcggaaacgg gccacctccg ccttgacggc
      181 ctcgggaggg acttgccagc ggccgacggc gaggtcggtg tcctccagga cgaacgcctt
      241 gccgatggcg atgcccggcg aagcggagat ccccttgaaa actttcgtct gagacggcga
      301 cggtgacggg ctcatggtct gtcttattcc tcgtcgaact tatttttgaa aagctgtgtc
      361 aactgctcca acagggcgtc ctcatcggga ccgtccgccg caatggtgag cttggcgccc
      421 ttttcggccg ccagcgtgag gatgcccatg atgctcttcc cgtccacttc ctggccgtct
      481 cggacgacgc tcacgttgca cttgaatttg tgcgtggttt ggacgaaaag cgccgccggc
      541 ctggcgtgga ggccgagttt attttcaacg atgaagacgg cttctttcat gacgactcat
      601 tcacggcgcg gcatccgaaa ccgcgcgccg gacctttccc tctcgtgcta gatgccgaaa
      661 aaataggcga aacagaaaca ggcggccaac atcgcataca ccacccgggt ggaagtgacg
      721 cgcagccgca acagaaccag cgtgaccgag ataaaaccgg ccatggcccc cagatggcgg
      781 ccgaaggcca gccacaacat cagcagcgcc cccagcgaaa caccggcccc gaggagcgtg
      841 gccccccgca cccacttctg cggccgcatg tccctcaaca gatggatgat ctccgacttg
      901 tgccggtacc cgaggaaaag gccgcccacg cgggccgcca aatgaaaact gttatacgcc
      961 gccaggaaga acaccggaac cagccaccag ctgaggcgcg cgtacgtcaa tcccagggcc
     1021 acggccagaa cgccggccaa aggccggagc gtggcccaaa aaacggtgtc acccagcgcg
     1081 gccagcggac cggacatggt cttcttggtg gccatgatct gttcccggcg gacggaaccg
     1141 gcggccgccc cctcctccat ccccgcgacc acgcccaaaa ccaggttcgc caaataggga
     1201 tgggtgttga aataatccag atgttccagc gccacgcccc tccgttgagg cggtcgatag
     1261 aaccgccgca acgccggcca aaggctgaac aaccacccca ggttctgcat cctttgaaaa
     1321 ttccagaggg tctggatgca aaaagaacgg aggaacattt tccacaggtc gcccgtggtc
     1381 agacgggccg gcgcgggaac ggcggcggtc acgggttcga gggtttgtcc gttgcccacg
     1441 ggaactccac ccggctgcgc tcgaaaaaat agttcacgac gacacaaaaa ccgatcagcg
     1501 gcagggagcg gccggtgaaa tccagcg
//
LOCUS       DAOTFD010000352         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_80723_length_1502_cov_4.636489, whole genome shotgun sequence.
ACCESSION   DAOTFD010000352 DAOTFD010000000
VERSION     DAOTFD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80723_length_1502_cov_4.636489"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="P5079_12060"
     CDS             <1..1377
                     /locus_tag="P5079_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HRY30756.1"
                     /translation="MVKAEGILTAIGGRTSHAAVVARGMGKPCVVGCGELKINEHAGT
                     LSVNGHSLKEGDYISIDGFTGEVIVGQVPTLPSEVIRVMRGEMDAKDAPLYDVFQTIM
                     KWADGVRKLGVRSNADVPRDAKMARALGAEGIGLCRTEHMFFAPERLPFMQEMILADT
                     EQDRRKALDKLLPFQKDDFKGLLKEMKGYGVTIRTLDPPLHEFLPKTREDAEELSRKI
                     GIPAEKIMAKTQELHEFNPMLGHRGCRLGITYPEITEMQARAIIMAACELKKEGITVV
                     PEIMIPLVGNIKELQDQTALVRRVADEEMKSSGVKVKYLLGTMIEVPRAALTAGEIAG
                     DAEFFSFGTNDLTQMTLGFSRDDAGKFTRVYLDKKIFKDDPFQTLDQTGVGRLMDLAV
                     KEGRKVRKDLKCGICGEHGGDPASVKFCHRVGLNYVSASPYRVPVARLAAAQAVVEEK
                     TGKSYTSK"
BASE COUNT          328 a          472 c          461 g          241 t
ORIGIN      
        1 atggtgaagg ccgagggaat ccttaccgcc atcggcggcc gcaccagtca tgccgcggtc
       61 gtcgcccgcg gcatgggcaa accgtgcgtg gtgggttgcg gcgaactgaa aatcaacgaa
      121 cacgccggca ccctctccgt caacggccat tcgttaaaag agggggacta catctccatc
      181 gacggtttca ccggcgaggt gatcgtcggc caggtgccca cgctcccgtc ggaagtgatc
      241 cgtgtgatgc gcggcgagat ggacgccaaa gacgcccctc tctacgacgt gtttcagacc
      301 atcatgaagt gggcggacgg cgtgcgtaag ctgggcgtgc gctccaacgc cgacgtgccg
      361 cgcgacgcca agatggcccg cgccctcggc gccgaaggca tcggcctctg ccgcaccgag
      421 cacatgttct tcgcgccgga acgcctgccg ttcatgcagg agatgatcct ggccgacacc
      481 gaacaggacc gtcgcaaggc cctcgacaag ctcctgccgt tccaaaagga cgacttcaag
      541 gggctgctca aggagatgaa agggtacggc gtcaccatcc gcacgttgga cccgcccttg
      601 cacgaattcc tgcccaagac ccgcgaagac gccgaggaac tttcccgcaa gatcggtatt
      661 ccggcggaaa agatcatggc caaaacccag gaactgcatg agttcaaccc catgctcggt
      721 catcgcggct gccggttggg gatcacctat ccggagatca ccgagatgca ggcccgcgcc
      781 atcatcatgg cggcctgcga gctgaaaaaa gaagggatca ccgtcgtgcc ggagatcatg
      841 atcccgctcg tcggaaacat caaggaactg caggatcaaa cagccctggt gcgccgcgtg
      901 gcggacgaag agatgaaatc ttcaggggtg aaggtgaaat acctgctcgg caccatgatc
      961 gaggtgccgc gcgccgccct caccgccgga gagatcgccg gcgatgcgga gttcttctcc
     1021 ttcggcacca acgacctgac ccagatgacc ctcggctttt cgcgagatga tgccggcaag
     1081 ttcacccggg tttatctgga caagaaaata ttcaaggacg acccgttcca gaccctcgac
     1141 cagaccggcg tgggccgcct catggacctg gccgtgaaag aaggccgcaa agtccgcaag
     1201 gacctgaaat gcggtatctg cggcgagcac ggcggtgacc cggccagcgt aaagttctgt
     1261 caccgcgtgg gtctgaacta cgtttccgcc agcccctacc gcgtgccggt ggcgcgtctg
     1321 gcggccgccc aagccgtcgt cgaggaaaaa accgggaaat cctacacctc gaagtgaccg
     1381 ggcccgacgg gctcggtcat ccggaggggc gctagccccg aaggagctcc gagggaaaca
     1441 gcttaaaaga ggcggggctt gaacgatatc gttcaagccc cgcctctttt atgggtaaaa
     1501 ag
//
LOCUS       DAOTFD010000353         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_83164_length_1477_cov_3.239100, whole genome shotgun sequence.
ACCESSION   DAOTFD010000353 DAOTFD010000000
VERSION     DAOTFD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83164_length_1477_cov_3.239100"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..712
                     /gene="pilO"
                     /locus_tag="P5079_12065"
     CDS             164..712
                     /gene="pilO"
                     /locus_tag="P5079_12065"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRY30757.1"
                     /translation="MALSKQQQQQIALIVMLGGGFLFVYWNYLLKPTNAKIVALETQV
                     TDILGQVETMKRTANRLPALQREYDALVAEVGETEKRLPKEKNLQEVLRIVTEQSMKN
                     KISVLSFAPGGDVQQNYCVEIPISLNITGQFHTLGKFLAILGQQERILTARDVRLSYT
                     PNEKKGHTISGSFILLAYTFKG"
     gene            718..1206
                     /locus_tag="P5079_12070"
     CDS             718..1206
                     /locus_tag="P5079_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30758.1"
                     /translation="MDRTSRGMFDSKAFTALLAGFLFAAVGAGTARTEEPATFSEKRA
                     IADSYVYKGGRDPFLPLAGQGTAWTAPTALSQEAEEFNVSSLELKGILTTRTGRWAVL
                     KTTGGGSYLVKDGKIQDSKRKPLKGYVGIVKEKSLVVIGPNNQVTELKLKKDEGGEET
                     PQ"
     gene            1223..1468
                     /locus_tag="P5079_12075"
     CDS             1223..1468
                     /locus_tag="P5079_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30759.1"
                     /translation="MNHLNDRAWPMLRRYANKSFSVSLVGGRLLLASLLLMDPVSLSR
                     LAAETASSPVAHIQQVTYENNTVRVSLDKEAPFHVFS"
BASE COUNT          340 a          477 c          396 g          264 t
ORIGIN      
        1 tctccaactt ttccatcgcc gactggctga cgaacctgaa cggttccccg ctgtgcggcc
       61 gggtgaccct ggggaccatc agcacccagg aaaccgccga ggggttgccg ggcatcttgt
      121 ccttcaccat gaccttcaac tatctgcgga aggaagcctg atcatggcgc tctccaaaca
      181 acagcaacaa caaatcgccc tcatcgtcat gttgggaggc gggttccttt ttgtctattg
      241 gaattatctc ctcaaaccga ccaacgccaa aatcgtcgcc ctcgaaacgc aggtgacgga
      301 catcctcggc caggtggaga ccatgaaacg caccgccaat cggctccctg ccctccagcg
      361 ggaatacgac gcgctggtgg cggaggtggg cgaaaccgag aaacgcctgc ccaaggagaa
      421 aaacctccag gaggtcctgc ggatcgtcac cgaacagtcc atgaagaaca agatttccgt
      481 cctaagcttc gcgcccggcg gcgacgtcca acagaactat tgcgtggaaa tccccatctc
      541 cctgaacatc accggccagt ttcatacgct tgggaaattc ctggccatcc tcggtcagca
      601 ggaacggata ttgaccgccc gcgacgtgcg gctcagctat acccccaacg aaaagaaggg
      661 ccacaccatc agcggctctt tcatcttgct ggcctacacc ttcaagggat gacgcccatg
      721 gaccggacct cccgcggaat gttcgactcc aaggccttca cggcgcttct ggccggattc
      781 ctgttcgccg ccgtcggcgc cggaacggcc cggacggaag aacccgcgac tttttcggaa
      841 aaacgcgcga tcgccgacag ctacgtctac aaaggggggc gcgacccctt cctcccgctc
      901 gccgggcagg gaaccgcctg gaccgcgccc accgcccttt cacaggaagc cgaggagttc
      961 aacgtctcat ccctggaact gaagggcatc ctgacgacgc ggacgggccg atgggcggtc
     1021 ctgaaaacga ccggcggcgg cagctatctg gtcaaagacg gcaagatcca ggattccaaa
     1081 cgaaaaccgc tcaagggcta tgtcggcatc gtcaaagaga agagtttggt cgtcatcggt
     1141 cccaacaatc aggtcacgga attaaaattg aaaaaagacg aaggtggaga ggaaactccg
     1201 caatgaggag gggtaagaaa aaatgaacca tctcaacgac cgcgcgtggc cgatgctgcg
     1261 ccgctacgcc aacaaatctt tttccgtttc cctggtggga ggacgcctcc tcttggcctc
     1321 tctccttttg atggaccccg tgtccctgag ccggctggcc gccgagaccg cctcctcgcc
     1381 ggtcgcgcac atccaacagg tgacttacga gaacaacacc gtgcgcgtct ccctcgacaa
     1441 agaagccccg ttccacgttt tctcgtaagt cacctgt
//
LOCUS       DAOTFD010000354         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_83834_length_1470_cov_4.359011, whole genome shotgun sequence.
ACCESSION   DAOTFD010000354 DAOTFD010000000
VERSION     DAOTFD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83834_length_1470_cov_4.359011"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..976
                     /gene="tpiA"
                     /locus_tag="P5079_12080"
     CDS             197..976
                     /gene="tpiA"
                     /locus_tag="P5079_12080"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY30760.1"
                     /translation="MARRPLIAGNWKMHKTVAESVELVQKLRESLSSVADRDILVAPP
                     FTVLAVVAEVLKGSQITVGAQNMNDNVQGAFTGEVSGALLKDVGCSHVILGHSERRQY
                     YGETDALVNKKLKLALDVGLTPIVCVGELLREREEGKTFTVIEKQVKESLGGLAPIQA
                     NKVVVAYEPVWAIGTGKTATPAQAQEVHSFIRKWIERLYGEPVASAVRILYGGSVKPD
                     NMDSLMAEADIDGGLVGGASLKAEDFTRIVRYQAVLSARAR"
     gene            991..>1470
                     /locus_tag="P5079_12085"
     CDS             991..>1470
                     /locus_tag="P5079_12085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HRY30761.1"
                     /translation="MAEDLSRDITQKIIQRLDRSQKPIVTNVSNRHFHCTQPTFEKLF
                     GAGKKPAKLRDLVQPGQFACQETVTVRGPKGEIKNVRMIGPIRNYDQVEVSRTDCFGL
                     GINPPVRDSGNLKGAAPITLVGPAGSVELKEGAIIALRHVHMHPKDAVDYGVKNMDYV
                     "
BASE COUNT          316 a          443 c          445 g          266 t
ORIGIN      
        1 gagggaatcg aattacaaag acgggagagg ggggaacacc tcctctcccg tctttcttat
       61 tttgcccgcc acttgccctt ccggaacgga tttcttaaaa tctcacggca ttgagagcga
      121 atctgtggtc attatgacca cggaggcgta aagtctctta tatagttact aaaactattt
      181 aaaggagtct cccctcatgg cgcgtcgtcc gctcatcgcc ggcaattgga aaatgcacaa
      241 gaccgtggcc gaatccgtgg aactcgttca gaagcttcgg gaatccctct cctcggtcgc
      301 cgaccgggac atcttggtgg ccccgccgtt caccgtgctc gcggtggtgg cggaggtgtt
      361 gaaggggtcc cagatcaccg tcggcgccca gaacatgaac gacaacgttc agggggcttt
      421 caccggcgag gtgtccggcg ccctgttgaa ggacgtgggc tgttcgcacg tgatcctcgg
      481 ccattccgaa cgacgccagt attacggcga gacggacgct ttggtgaaca aaaaattgaa
      541 attggcgctg gacgtcggcc tgacgcccat cgtgtgcgtg ggggaactcc tgcgcgaacg
      601 ggaggaaggc aaaacgttta cggtgatcga aaaacaggtg aaggaaagcc tcggcggcct
      661 ggcgccgatc caggccaaca aggtcgtcgt ggcctacgag ccggtgtggg ccatcggaac
      721 cgggaaaacc gccacgcccg cccaggccca ggaggtccac tccttcatcc gcaaatggat
      781 cgaacggctt tacggcgaac ccgtggcgtc ggccgtgcgg atcctttacg gcgggtccgt
      841 caagccggac aacatggact cgctcatggc cgaggccgac atcgacggcg ggctggtggg
      901 cggggcgagc ctgaaggcgg aagattttac gcgtatcgtc agatatcagg ccgttttgtc
      961 ggcccgcgca aggtagagga ggcaaccccc atggccgaag acttgagccg cgacatcacc
     1021 cagaaaatca tccagcgcct ggaccgctcc cagaagccca tcgtcaccaa cgtctccaac
     1081 cggcatttcc attgcactca gccgacgttc gaaaaacttt tcggcgccgg caaaaagccc
     1141 gccaagctgc gcgacctcgt ccagccgggc cagttcgcct gccaggagac cgtcaccgtc
     1201 cgcggcccca aaggcgagat caagaacgtg cgcatgatcg ggccgatacg caattacgac
     1261 caggtggaag tttcccgcac ggactgtttc ggtctgggga tcaatccgcc ggtgcgggat
     1321 tccggcaacc tcaagggcgc cgcccccatc accctggtcg gtccggccgg gtccgtcgaa
     1381 ctgaaagagg gggccatcat cgccttgcgg cacgtgcaca tgcaccccaa agacgcggtg
     1441 gattatggtg tcaagaacat ggattacgtc
//
LOCUS       DAOTFD010000355         1429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_87982_length_1429_cov_4.663755, whole genome shotgun sequence.
ACCESSION   DAOTFD010000355 DAOTFD010000000
VERSION     DAOTFD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87982_length_1429_cov_4.663755"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="P5079_12090"
     CDS             <1..1147
                     /locus_tag="P5079_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30762.1"
                     /translation="LYFEDLPSPDLRLRYLVGEGARARVVESGRDVNLLRAALLFAGR
                     DLPTGTDVLDDRALAALLEGFAFSDRQELAELHARLAAAVRSRLGAESDHLAQGRSTD
                     GEGPAELNFVRALGFLHGLGHDLRGRAPADLDVLVDTFNAARRALGRRALSEGDYQGL
                     ENGYRRGWLSGRWAWEQTAVHPFSAWGEAVLPVTAETPGSPTVDLYDITRVLQTPADQ
                     PLPPSAESLLARLADSLASVDALPTEQEKRQALGRMHVRWLSNALSGSPAEGLAFLEK
                     RLAAQGYKKGLGHVARLAARSRGVAGTTIDTLQVYRDIASQTPRALLQILTDDVQRWT
                     ALSEEDQRRHQLRVAFLEILKDGFRIVRPLAELENAFREIQLLKIQA"
     gene            1235..>1429
                     /locus_tag="P5079_12095"
     CDS             1235..>1429
                     /locus_tag="P5079_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY30763.1"
                     /translation="MTFSESDTQRYARQLILPQVGEAGQNRLRAAKVLCVGAGGLGSA
                     VAYYLTAAGVGTLGLLDNDAV"
BASE COUNT          226 a          435 c          496 g          272 t
ORIGIN      
        1 tttgtatttt gaggatctcc cttccccgga cctgagattg cgctatctcg tgggggaagg
       61 ggcccgggcc cgggtggtgg aatcgggccg ggacgtgaac ctcctgcgcg ccgcgctgct
      121 ctttgccggc cgggatttgc cgacgggcac cgatgttttg gatgaccggg cgttggccgc
      181 tttgctggag ggctttgctt tctccgaccg gcaggagctt gcggaactcc acgcccgcct
      241 cgcggcggcg gtccggtccc gtttgggcgc cgagagcgat catttggccc aaggccgttc
      301 gacggacggc gaaggcccgg cggaactgaa ttttgttcgg gctctcgggt tcctgcacgg
      361 cctcggccat gatctgcgcg ggagggcgcc ggcggatctg gatgttttgg tggacacgtt
      421 caacgcggcc cggcgcgctc tgggccgccg cgcgttgagc gaaggagatt atcaaggctt
      481 ggaaaatggc tatcggcggg gatggctctc cggtcgatgg gcttgggaac agaccgcggt
      541 tcatccgttc tcggcgtggg gggaggcggt cctgccggtg acggccgaga cgccaggctc
      601 tccgacggtg gacctctacg atatcacccg ggtcctgcag accccggcgg accagccgtt
      661 gccgccgtcg gcggagagcc tgctggcccg gttggcggat tccctcgcct cggtggacgc
      721 tctgccgacg gaacaggaga agcggcaggc gttgggccgc atgcacgtcc gctggttgtc
      781 gaacgccctg tccggttcgc ccgccgaggg gttggccttt ctggaaaaac gcctggccgc
      841 ccaaggatat aaaaaagggt tgggacacgt ggcccgattg gcggcccgaa gccgcggtgt
      901 cgccgggacg acgatagaca cccttcaggt ttaccgggac atcgcgtcgc agactccccg
      961 ggctctcctt caaatcctca ccgacgacgt ccaacgatgg acggccctct ccgaggaaga
     1021 ccagcggcgg catcaactgc gcgtggcttt cctggagatc ctcaaagacg ggttccgtat
     1081 cgtgcggcct ctggcggaac tcgaaaacgc tttccgcgag atccagctcc tcaagataca
     1141 ggcgtaaacc ggcgcttcgc cgccggatga aaatcgggcg gcaaaagtgc tatccttgac
     1201 ctcgtaagat tctccggtcc gacatccgaa aacaatgact ttttccgaat ccgatacgca
     1261 acgttacgcg cgccagctca tcctgccgca ggtgggagag gccggacaga accgtctccg
     1321 cgcggcgaag gtcctctgtg tgggcgcggg ggggctgggt tcggcggtgg cctattatct
     1381 gacggccgcg ggggtcggga cgctcggcct tttggataac gatgcggtg
//
LOCUS       DAOTFD010000356         1406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_90624_length_1406_cov_4.237602, whole genome shotgun sequence.
ACCESSION   DAOTFD010000356 DAOTFD010000000
VERSION     DAOTFD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90624_length_1406_cov_4.237602"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="P5079_12100"
     CDS             complement(<1..1022)
                     /locus_tag="P5079_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30764.1"
                     /translation="MEPPRGDAKSISGLRHLFLSERTKETAPYLLLFALNLLLFLLFY
                     GKFGDILIDSSREATVPWRILHGQVPYRDFQYEYGPFSAYLFALLYKLFGVHLAVLQG
                     TGLAVSVLVSGLIHRLCRIFIERTPSLLASVFFVFVFAFQHYFPNNNFNFIFPYSYSA
                     TFGIFFLLTLALTAHRMTIEKTARGPILMGFLVSLTFLTKIEIAAAATVFVTLFFSLP
                     PAASSPRRKDRILFLSALFFPLVPVALYFSAKTDLAAYLRDEIFHLALRGVQTPLARR
                     FLGTDRPVAHVFEILLVCLQYALFVLFCSLLDRLLERWARHPSRGTGLFLRGTCFLLA
                     AGAAYGT"
     gene            1217..>1406
                     /locus_tag="P5079_12105"
     CDS             1217..>1406
                     /locus_tag="P5079_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRY30765.1"
                     /translation="MSKEDKMKALQLALSQIEKQYGKDSIMKLGEKFAKVQVEVIPTG
                     ALPLDVALGVGGLPRGRVV"
BASE COUNT          353 a          323 c          501 g          229 t
ORIGIN      
        1 gtcccgtagg ccgcccccgc cgccaaaaga aaacaagttc cccgcaaaaa caggccggtc
       61 ccccgggagg gatgccgggc ccaacgttcg aggagtcgat ccaataggga acagaacaaa
      121 acgaaaagcg catactggag gcataccaga agaatctcga aaacatgggc gacgggccgg
      181 tcggtgccga ggaagcgccg ggccaggggg gtctgaacgc cgcggagggc caggtgaaag
      241 atctcgtctc ttaggtaggc ggcaaggtcg gttttcgcgc tgaaataaag agccaccggg
      301 accagcggga agaacagcgc cgacaggaag aggatccggt ctttgcgccg gggggaggac
      361 gccgccgggg ggagggaaaa gaacagcgtc acaaagaccg tggcggcggc ggcgatctcg
      421 attttcgtca ggaaggtgag ggacaccagg aagcccataa ggatcgggcc cctcgcggtt
      481 ttttcaatcg tcatccgatg cgcggtcagc gccagggtca gaagaaaaaa gatcccgaag
      541 gtggcggaat aggaataggg gaatatgaaa ttgaagttgt tgttcgggaa gtagtgttgg
      601 aaggcgaaaa cgaagacgaa gaagacggag gcgaggagcg acggggtgcg ctcgatgaat
      661 atccggcaga ggcggtggat caggccgctc accagcaccg acacggcgag ccccgttccc
      721 tgaaggacgg ccaggtgcac gccgaagagt ttgtaaagaa gggcgaagag gtaggccgag
      781 aaaggcccgt attcgtactg gaaatcgcgg taggggacct gaccgtggag gatgcgccac
      841 gggacggtgg cttcgcggga cgagtcgatg aggatatcgc cgaactttcc gtaaaagagg
      901 aggaagagga gaaggttgag cgcaaaaagg aggagatagg gggcggtctc tttcgtccgt
      961 tccgacagga agagatgccg gaggccggaa atggatttcg catcgccgcg agggggttcc
     1021 atgacgggct caagatacca taatttttag ttcgaaacgt aggggtcttc cgcggcctag
     1081 ccaagtcggg cttctttcga tttcgaatcg tcgagcgttt tttttgtgcg aatgacaaag
     1141 ccgctgcaat ttggtacgat gcgaccataa caacccaccg gttgttccta gctaaaatcc
     1201 ccatcggagg tgtgcggtga gcaaagaaga caagatgaaa gcgcttcagt tggctctttc
     1261 ccaaatcgag aaacaatacg ggaaggactc gatcatgaaa ctgggggaga agttcgccaa
     1321 ggtgcaggtg gaggtgattc cgacgggggc gttgccgctg gacgtggcgt tgggggtggg
     1381 ggggctgccg cgggggcggg tggttg
//
LOCUS       DAOTFD010000357         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_92402_length_1390_cov_3.490637, whole genome shotgun sequence.
ACCESSION   DAOTFD010000357 DAOTFD010000000
VERSION     DAOTFD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92402_length_1390_cov_3.490637"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="P5079_12110"
     CDS             complement(<1..759)
                     /locus_tag="P5079_12110"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRY30766.1"
                     /translation="MRSFTEGAMNSERRPIAEIVQGGLKTVLAAAFLAAVAAGPRVWA
                     AGGQGGAPGQFLSWGAGARSMGMGKAFTAVADDASATYWNPAAMVQMERKELQAMHVS
                     LFEDTSFDFVSYVHPTARAGTLGLNFTRLVSGGFEKIAITLDPTSQEIVKLQKLGDFS
                     DINQAYGISYGRQFVENMSIGVTGKVINHTIDDFRQNFFSLDAAFFAKELIPHYRLAL
                     MAQNVVTQVSGETDDKLPLTLRVGHGLGLRRVHQQ"
     gene            complement(743..1138)
                     /locus_tag="P5079_12115"
     CDS             complement(743..1138)
                     /locus_tag="P5079_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRY30767.1"
                     /translation="MNRKSVQMIVGAAMLLGWSVWGVYGIPTPRLESLANYPNPFDSR
                     LTATSIVYSLPTDAAVTVRLYDLLGRPVREWNFHAGENGARNGENRLSWDGTDESGAK
                     VSAGGYVCQVLVEQESGLIQGIRKIAVIH"
BASE COUNT          266 a          451 c          422 g          251 t
ORIGIN      
        1 ctgctggtgg acccggcgca ggccgaggcc atggcccacg cgcagcgtca ggggcagttt
       61 atcgtcggtc tcgccggaca cctgcgtcac cacgttctgc gccatcaagg ccagccggta
      121 atgggggatg agctccttgg cgaagaacgc ggcgtccaac gagaaaaaat tctgccggaa
      181 atcgtcgatg gtgtggttga tcaccttgcc cgtcacgccg atggacatgt tctccacgaa
      241 ctgccggccg taggagatgc cgtaggcctg gttgatatcc gagaaatccc ccagtttctg
      301 aagtttcacg atctcctgag aggtggggtc gagggtgatg gcgatctttt cgaatccgcc
      361 ggaaaccaaa cgggtgaagt tcaggcccag ggtgcccgca cgggcggtgg ggtgcacgta
      421 agaaacgaaa tcaaagctgg tgtcctcgaa caacgacacg tgcatggcct gcagttcctt
      481 gcgttccatc tggaccatgg ccgccgggtt ccagtaggtg gcggaggcgt cgtcggccac
      541 ggcggtgaag gccttcccca tgcccatgct gcgcgccccg gcgccccagg agaggaactg
      601 tcccggcgcg ccgccctgcc cgcccgccgc ccagacccgc gggcccgccg ccaccgccgc
      661 caaaaacgcc gccgcaagga ccgttttcag gcccccttgg acgatttcgg cgatcggacg
      721 gcgttcgctg ttcatggcgc cctcagtgaa tgaccgcaat cttccggatc ccctggatca
      781 atccggattc ttgttccacc agcacctggc agacataccc gccggcggac accttcgcgc
      841 cggactcgtc ggttccgtcc cagctcagcc ggttttcgcc gttccgggcg ccgttctctc
      901 cggcgtggaa attccactcg cgcaccggcc ggccgagaag gtcgtacagg cggaccgtca
      961 ccgcggcgtc ggtcggcagg gaatacacga tggaggtggc ggtgagacgg gaatcaaacg
     1021 ggttgggata gttggccagg gactccagcc ggggggtggg aatgccgtaa acgccccaga
     1081 cgctccatcc caggagcatc gcggcgccga cgatcatttg cacacttttt cgattcatcc
     1141 tcgtcccccg taaaaaaacg gccaaccgat tgacaagctc tccgctcgca accacggttg
     1201 accgtcgtct acgggaaaag aggccatgaa tccctcaacg ttgttattat gaagaactga
     1261 tacgttctcc ccacaacggt gaaagattca ttcctgtttc cacgcgcggg catccgacac
     1321 ccgccatgcc ggacacgact gacgtgtccg gttccttcgg caacccggcc acccggaaga
     1381 agtgctgagg
//
LOCUS       DAOTFD010000358         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_93775_length_1378_cov_3.883598, whole genome shotgun sequence.
ACCESSION   DAOTFD010000358 DAOTFD010000000
VERSION     DAOTFD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93775_length_1378_cov_3.883598"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="P5079_12120"
     CDS             complement(<1..1346)
                     /locus_tag="P5079_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY30768.1"
                     /translation="MLITGQTSKILSFKISQFIKHFMRTYEEALKDWKGNALNGTWTP
                     STLATAPNTTNSNWSNQIDWLHMVTDPNTNNVMLSAVSSSRDLRSIRWQPATNDWTTG
                     TFDHTTNNASDYNRQSFAFEYDRHDTYPPSLVSNLPAGTDAAWRSSNTATYNVDVYDT
                     GGSHLDKFQARASTSTGGAGPFHPDWTDVLASLSPADAHTTDWSFPTSFFDDLPEGVN
                     YVSLRAQDVLGNYSTALTDAFFVRKDTTPPAAPSLLTPADNAYSSTNSVTFDWSDVSD
                     TVSGIKDYSMDVSTDPAFGSFAFSHTGALSNAATTLASGKYYWRVTARDNAMNSSVAA
                     STFAVTVDTAAPAVAVGGGLIGGDTAWRRENPGALYDVNFNDSGHSLLKEARYSVWTS
                     TGRSGTQLISFANGLIADNIAADAYSTDWGLDFSFLENGMNYVSVQVKDFAGNATT"
BASE COUNT          242 a          356 c          509 g          271 t
ORIGIN      
        1 taggtcgtgg cgttgcccgc gaaatctttc acctgaacgg agacgtagtt catcccgttt
       61 tccaggaaac tgaaatcaag tccccaatcc gtcgaatagg cgtcagcggc gatgttgtcc
      121 gcgatgaggc cgttggcgaa ggagatgagc tgtgtgccgg agcggccggt ggaggtccat
      181 accgagtagc gcgcctcctt cagcagagaa tgtcccgaat cgttgaagtt gacgtcgtag
      241 agggcgccgg gattctcccg ccgccaggcg gtatcgccgc cgatgagtcc gcctccgacc
      301 gcgaccgcgg gggcggcggt gtccacggtg acggcgaagg tcgacgcggc cacggacgag
      361 ttcatggcgt tgtcccgcgc cgttacccgc caataatatt ttccggaggc gagtgtcgtc
      421 gcggcgttgg aaagggcgcc ggtatggctg aacgcaaagg agccgaaggc ggggtcggtg
      481 gagacatcca tcgaatagtc cttgatgccg gacaccgtgt cgctcacgtc cgaccagtca
      541 aacgtcacgg agttggtgga gctgtaggcg ttgtccgccg gcgtcagaag ggagggggcg
      601 gccggtgggg tggtgtcctt tcggacgaag aacgcgtccg tcagggccgt ggaatagttc
      661 cccagcacgt cctgcgcccg gaggctgacg taattcacac cctccggcag gtcgtcgaaa
      721 aaggaggttg ggaacgacca atccgtggtg tgggcgtcgg cggggctgag ggaggcgagc
      781 acatccgtcc agtcggggtg gaacgggccg gcgccgccgg tggaggtgct ggcccgcgct
      841 tggaatttgt cgaggtgaga accgccggtg tcatacacgt ccacgttgta ggtggcggtg
      901 ttggaggaac gccatgcggc gtccgtcccg gccggcaggt tgctgaccag cgacggcggg
      961 taggtgtcgt gacggtcgta ttcgaaagcg aaggattgac ggttgtaatc cgaggcgttg
     1021 ttggtggtat ggtcgaacgt gccggtggtc cagtcgttgg tggcgggttg ccagcggatg
     1081 gaacgcaggt cgcgcgacga cgaaacggcc gagagcatga cgttgttggt gttgggatcg
     1141 gtcaccatgt ggagccaatc gatctggttg ctccagttgg agttggtggt gttgggggcg
     1201 gtggccagcg tggagggcgt ccaggtgccg ttcagggcat tgcccttcca gtccttcaag
     1261 gcctcctcgt aggtgcgcat gaagtgcttg atgaactggg agatcttgaa cgacaagatc
     1321 ttcgaggtct ggccggtgat aagcacggcg aacttgcatg gcccgctggt ttccatca
//
LOCUS       DAOTFD010000359         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_3112
            NODE_95958_length_1360_cov_4.616858, whole genome shotgun sequence.
ACCESSION   DAOTFD010000359 DAOTFD010000000
VERSION     DAOTFD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868136
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:02:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,426
            CDSs (total)                      :: 2,384
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95958_length_1360_cov_4.616858"
                     /isolate="AMR_MDS_3112"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="P5079_12125"
     CDS             <1..772
                     /locus_tag="P5079_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY30769.1"
                     /translation="AYHSYDEIDFSASPAKLKASGEPLREEVEKMSKSKKNVVSPDDI
                     IARYGADAMRLYEMFMGDFEQPKPWDMRAVEGITRFLQKIWRLAEGVRAGKTVADGVH
                     LRARHKTIQAVTERVENFKFNTAIAALMEFANLLSGGASRSDVETLALLLSPFAPHLA
                     EEIWEAAGKKGYISAAAWPAADPAHLKDDSVEYPVQINGKLRDKLRAATTATEEEVLA
                     AARALPKVQEFLKNQEVLKEIFIAGRMVSFVSRPQNGK"
     gene            800..>1360
                     /locus_tag="P5079_12130"
     CDS             800..>1360
                     /locus_tag="P5079_12130"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRY30770.1"
                     /translation="MKRMERAGLLLAFAGLSLLIGCGEAGMYTPAPMLLPPHIRKIAV
                     RPVANKTQFFGLEEKLRLRLEEEFIRDGRLPYVNNETEADGVVVGEIVRYIKEPISYD
                     ANHVVEEYKLWVIMNLQFIDRVNNVILWQEPRMDQEYRYFVETKPGGVTEDEARELLW
                     ELFSRDIVKRTIEGFGSVSGASQRKVS"
BASE COUNT          291 a          423 c          419 g          227 t
ORIGIN      
        1 cgcctaccac tcctacgacg agatcgattt cagcgcctcg ccggccaagt tgaaggcctc
       61 cggcgaaccg ctccgcgagg aagtggagaa gatgtccaaa tcgaagaaga acgtcgtcag
      121 tcccgacgac atcatcgccc ggtacggcgc cgacgccatg cggctctacg agatgttcat
      181 gggggatttc gaacagccca aaccctggga catgcgggcc gtggagggca tcacgcgttt
      241 cctgcagaaa atctggcggc tggccgaagg cgtccgcgcc ggcaagaccg tggccgacgg
      301 cgtccacctc cgcgcccggc acaaaaccat ccaggccgtc acggaacgcg tcgagaactt
      361 caaattcaac accgccatcg ccgccctgat ggagttcgcc aacctccttt ccggcggggc
      421 ctcccggtcg gacgtcgaaa cgctggccct cttgctctcg cctttcgccc cgcacctggc
      481 ggaggaaatc tgggaagccg ccgggaagaa gggatacatc tcggcggcgg cctggcccgc
      541 ggcggacccg gcccatttga aagacgattc cgtcgaatat cccgtgcaga tcaatgggaa
      601 actgcgcgac aagctgcgcg cggcgacgac cgccacggag gaggaagtgc tggccgcggc
      661 gcgcgccctg cccaaggtgc aggaatttct gaaaaaccag gaagtgctga aagagatttt
      721 tatcgccgga cggatggtca gtttcgtctc ccgcccgcaa aacggtaaat agcgccagtc
      781 gaccgtaagg agaattgcca tgaaacgaat ggaacgtgcc gggttgctct tggccttcgc
      841 ggggttgtct ctgctgatcg gatgcggcga ggcggggatg tatacccctg cgccgatgct
      901 cctgccgccc cacatccgca aaatcgcggt gcggcccgtg gccaacaaga cacagttttt
      961 cggtctggaa gaaaaactcc gcctgcggct cgaagaggag ttcatccgcg acggccgcct
     1021 tccctacgtg aacaacgaaa ccgaggcgga cggcgtggtg gtgggggaga tcgtccgtta
     1081 catcaaggaa cccatctcct acgacgccaa ccacgtggtg gaggaataca agctctgggt
     1141 catcatgaac ctgcagttca tcgaccgcgt caacaacgtc atcctctggc aggaaccgcg
     1201 catggaccag gaataccggt atttcgtgga gacgaagccg ggcggcgtga ccgaggacga
     1261 agcccgggaa cttctctggg aactcttctc ccgtgatatc gtgaagcgga ccatcgaggg
     1321 gttcggttcc gtctccgggg ccagccagcg aaaagtgtcc
//
